LOCUS       DBNGWZ010000001         1950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1889330, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000001 DBNGWZ010000000
VERSION     DBNGWZ010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1889330"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC16CX_00005"
     CDS             complement(<1..368)
                     /locus_tag="AC16CX_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3010928.1"
                     /translation="MYYSHFGLNQAPFKITPNTEFFFSGGNRGPILEALVYAITHGEG
                     IIKVTGEVGSGKTMLCHMLPTRLPAHIETVYIANPSFSPEEILHAIAFDLQLAVDRGA
                     LRLEVMQALHDFLLGHYAEG"
     gene            complement(488..>1950)
                     /locus_tag="AC16CX_00010"
     CDS             complement(488..>1950)
                     /locus_tag="AC16CX_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3010929.1"
                     /translation="EQEKPDLLIVDDDPLITDTLNFVLSRDFTVYVADSRARVKSLLT
                     QLDNPPQLALIDLGLPPTPHRPDEGFRLISELIAHSPGIKIVVLSGQSDETNARHART
                     LGAIDFVAKPADPETLRNLLNRALEVREAELGAAAEGGLMGKSLLVEKLRQQISQFAN
                     APFPVLIEGESGSGKELVATALHRMSQRAARPFLALNCAAISPTLVEPTLFGYAKGSF
                     TGATTSRSGYFEDAKDSTLFLDEIGELPLELQAKLLRVLENGEYQRVGETQSRVSNAR
                     VVAATNRDMRQEIRAGRFRADLYHRLSVFTISVPPLRDLGEDKCVLLDHYREFYARQA
                     RVEPFELDADAMGGWLDYSFPGNVRELRNIVIRLTTKCAGQKVGSPQLQAELDMESLD
                     IELPGLAAGQDFKAVLEAAKRHLQLHKNFSLDNTLSQWERGYVEAALMITQGNLTQAA
                     KLLGIHRTTLYSRMQGYGNPGETAKAPRPAATKETR"
BASE COUNT          316 a          608 c          655 g          371 t
ORIGIN      
        1 ttgccctcgg cgtagtgccc gagcaggaaa tcgtgcagcg cctgcatgac ttcgaggcgc
       61 agcgcgccgc ggtccaccgc cagctgcaga tcgaaggcga tcgcgtgcag tatttcttcg
      121 ggagaaaagc tgggattggc gatgtaaacg gtctcgatgt gggcgggcag ccgtgtcggc
      181 agcatgtggc acagcatggt cttgccgctg ccgacctcgc cggtcacctt gatgatgccc
      241 tcgccgtggg tgatggcgta gaccagcgcc tccaggatcg ggccgcggtt gccgccgctg
      301 aagaaaaact cggtattcgg cgtaatcttg aacggcgcct ggttcagtcc gaaatggctg
      361 taatacatcg ccggtcccct aggaattcgc cggacctgat tcgggcgaat tccttaatga
      421 aaaccgcctc cctccaacgg aagcgggcag catctcaaca agttccatgc tgttcatttg
      481 cggatggcta ccgggtctct tttgttgcag ccggccgcgg cgctttcgcc gtctcgcccg
      541 ggttgccgta gccctgcatc ctgctgtaga gggtggtgcg gtggatgccg agcagcttcg
      601 cggcctgggt caggttgccc tgcgtgatca tgagcgccgc ctcgacgtaa ccgcgctccc
      661 actgcgaaag cgtgttgtcg agactgaagt tcttgtgcag ttgcaagtgg cgcttggccg
      721 cttccagcac cgccttgaaa tcctggcccg cagcgagccc cggcagctcg atgtcgaggc
      781 tttccatgtc gagctccgcc tgcagctggg gcgagccgac cttctgcccg gcgcatttcg
      841 tggtcagccg gatcacgatg ttgcgtagct cccggacgtt gccggggaag ctgtagtcga
      901 gccagccgcc catcgcgtcc gcatcgagct cgaacggttc cactcgcgcc tggcgcgcgt
      961 agaactcgcg ataatggtcg agcaggacgc acttgtcctc gccgagatcg cgcagcggcg
     1021 gcacgctgat cgtgaacacc gacagccggt ggtagaggtc ggcgcggaag cgcccggcgc
     1081 ggatctcctg ccgcatgtcg cggttggtag cggccaccac gcgcgcattg gacacgcggc
     1141 tctgggtttc gcccactcgc tggtattcgc cgttttccag gacgcgcagg agcttcgcct
     1201 gcagctcgag ggggagttcc ccgatctcgt cgaggaacag cgtggaatcc ttggcgtcct
     1261 cgaaatagcc cgagcgcgag gtcgtggcgc cggtgaacga gcccttggcg tagccgaaca
     1321 gcgtcggctc caccagcgtc ggcgaaatcg ccgcgcagtt gagcgcaagg aacggccgcg
     1381 cggcgcgctg gctcatgcgg tgcagcgcgg tggcgaccag ctccttgccg ctgccggact
     1441 cgccctcgat cagcaccggg aagggggcat tggcgaactg ggagatctgc tgccgcagct
     1501 tctcgaccag caggctcttg cccatcaatc cgccttccgc ggccgcgccg agctcggctt
     1561 cgcgcacctc cagcgcgcgg ttgagcaagt tcctcagcgt ttcgggatct gctggtttag
     1621 caacgaaatc aatggcgcca agggtgcggg cgtggcgcgc gttggtttca tcgctctggc
     1681 ccgacagcac cacgatcttg atgccgggcg aatgcgcgat cagctcgctg atcaggcgga
     1741 acccctcgtc aggacggtgc ggggtgggcg gaagcccgag gtcgatcagg gcgagctgcg
     1801 gcgggttgtc gagctgggtg agaaggctct tcacgcgagc gcgggaatcc gcgacgtaca
     1861 cggtgaaatc gcggctcagc acgaagttga gcgtgtccgt gatcagcgga tcgtcgtcga
     1921 cgatcagcag atcgggcttc tcttgttcag
//
LOCUS       DBNGWZ010000002         3796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2519152, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000002 DBNGWZ010000000
VERSION     DBNGWZ010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2519152"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC16CX_00015"
     CDS             <1..530
                     /locus_tag="AC16CX_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3010930.1"
                     /translation="RPGQFNYSSSGTGGGAHLAVELFRTMAGFDMVHIPYKGSAQATS
                     EVVAGHVQMSFSQPVVMLPHARSGRIKVLGVTSLKPLASWPEAPPVAEAGLPGFEASS
                     WQGVLVPARTPRTIIERLHREIVKALHSPEVERRLIAEGSEIGGVSPEAFAAHIRREI
                     AKWTRVVKEAGIRVE"
     gene            complement(604..1359)
                     /locus_tag="AC16CX_00020"
     CDS             complement(604..1359)
                     /locus_tag="AC16CX_00020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3010931.1"
                     /translation="MDLQLTGKTALVTGASRGIGRAIATGLAREGVKVAIAARRVNLL
                     EELAREIVSARGAEPLIIETDLYQEDSAETLARSALAGLGRVDILVNAAGGSRPFPFE
                     ATKEQWTEGILVNFIRLRELSHAVIPGMIAQRWGRIINITGTSEPRGLNAANSAKAAV
                     HAWSKGLSREMAKHGITINSIQPGRIMSEQIVRFHPTEEDRRRFAEENIPMGRFGEPE
                     ELANLAVFLASPRAGYITGTVTPVDGGMSRFAF"
     gene            1493..>3796
                     /locus_tag="AC16CX_00025"
     CDS             1493..>3796
                     /locus_tag="AC16CX_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3010932.1"
                     /translation="MGVPYGYCYTKRRQVDSFSGEDDFEMNAEHTLITSIPTIAARLR
                     AADDIDRTLAEISQDICVLFEADRLTIYRVSEDRTLIVAKVKTGLDSFEDLKLPISAE
                     YSIAGYVALNRRRVNIRDVYNVDELNSFDPPVYFFRAVDRSTGYKTKQVLAAPIFDGA
                     DSNEVIGVVQLINTLSGNPFQKSAEERVVELCKALAVGFRQLEKPRQVGTKTKYDHLV
                     IDGVVTAAELELAIRLARGKGINIEQVLKDEFKVTDSALGLALSKFFGLPYEPFDPDR
                     AKPTALLRKLKRTFVNSQAWVPITEDDQGMVIVTTDPERVISSRIVSQVFPRKKVTYR
                     VCSNSEFAATVEHFYRLEGAPASVDTIIKRMDQDTSEARGVVVEEKTEPDSATVQLVN
                     KIIVEAHQMRASDIHVEPRQGGVKSKIRFRIDGSLVDYTDIPASHHAQVVARLKIMSD
                     LDIANKREPQDGKIAFKQFVPSLDIELRVATIPTVGGKEDVVMRILTAGRPVPLEQLG
                     LSQHNLRILKSLISKPYGLFLVCGPTGSGKTTTLHSVLGHINTPETKIWTAEDPVEIT
                     QESLRQVQINPRADLTFARAMRAFLRADPDVIMVGEMRDRETAAIGIEASLTGHRVLA
                     TIHTNSAPESIIRLLDMGMDPFNFADALMGIMAQRLAKRLCAKCKKPHVATLEEIGLM
                     LTEYNQDLKDTDRFKSDAKAAYEAVHAEWTKNYAYDKGQFILYEPVGCEACRGTGYSG
                     RVALHELLAVTDAIKKNIQEHARVAEVL"
BASE COUNT          787 a         1124 c         1171 g          714 t
ORIGIN      
        1 cgcggccggg acagttcaat tattcttctt caggaacggg aggcggggcg cacttggcgg
       61 tggagctgtt ccggaccatg gccggattcg acatggtgca cattccatac aagggctctg
      121 ctcaggccac cagtgaagtt gtggccggtc acgtgcagat gagtttcagc cagcccgtcg
      181 tcatgctgcc ccacgcaagg tccggacgga tcaaggtgct gggtgtaacg agcctgaagc
      241 cgctggcgtc atggccggag gcaccaccgg tggcggaagc ggggctgccg ggtttcgagg
      301 catcgtcctg gcagggtgtg ctcgtgccgg cgaggacgcc gagaacgatt atcgaacgac
      361 tgcaccggga aatcgtcaag gcactgcact cgccggaggt ggagcgccgg ctgatcgccg
      421 agggctcgga aatcggcggg gtctctcccg aggcgttcgc cgcccatatc cggagggaaa
      481 tcgcgaaatg gacccgcgtc gtgaaggagg cgggcatcag ggtcgagtag ccgtcagccg
      541 tcagctctca gcttgaaccg aatttggctg atcgctgaaa gctgatagct gaacgccagt
      601 tccctagaac gcgaacctgc tcattccacc atcgactgga gtgaccgttc ccgtgatata
      661 gcccgcccgc ggcgaggcaa ggaacacggc gaggttggca agttcctccg gctctccgaa
      721 gcggcccatc gggatgttct cctcggcgaa ccgcctgcgg tcttcctcag tgggatggaa
      781 gcgcacgatc tgctcgctca tgatgcggcc cggctggatc gagttgatgg tgatgccgtg
      841 ttttgccatc tcgcgcgaca gtcccttgga ccacgcgtgc accgccgctt tggccgagtt
      901 cgccgcgttg aggccgcggg gttccgacgt tcccgtgatg ttgatgatgc gtccccagcg
      961 ctgcgcgatc atcccgggta tcaccgcatg ggacagctcg cgcagccgga taaagttcac
     1021 cagtatgccc tcggtccact gctccttggt cgcctcgaac ggaaaggggc ggctgccgcc
     1081 ggcggcattg accaggatgt cgacgcgtcc cagccccgcc agcgccgatc gcgccagcgt
     1141 ctcagccgag tcttcctggt aaaggtcggt ttcgatgatc agcggttcgg cgccgcgcgc
     1201 ggatacgatt tcccgggcga gttcttccag gaggtttacc cgccgcgcgg cgatcgcgac
     1261 tttcacgccc tcacgcgcca ggcctgtggc tatggcgcgt ccaatgccgc gcgaagcgcc
     1321 ggtgacgagt gcggtcttgc cggtgagctg gaggtccatt gccatctcct ctaagcggaa
     1381 gatgcattca cggcggggca cggccccgcg ccacaggtaa aaagctctaa tggagccgaa
     1441 tcggcgcgga gtagcacccg ctcccgcctc ttcatgagcg aatcgagccc ggatgggcgt
     1501 gccatacggc tattgctata ccaaacgtcg gcaggtagat tccttctctg gcgaggacga
     1561 ttttgaaatg aacgctgaac acactcttat cacgagcatc ccgaccatcg ccgccaggct
     1621 tcgtgcggcc gatgatatag accggacatt agctgaaatc agccaggata tctgcgtcct
     1681 cttcgaggcg gatcgcctga cgatttaccg ggtcagcgaa gaccggacct tgattgtcgc
     1741 gaaggtcaag accggtctcg attcgtttga ggacttgaag ctcccgattt ccgcggaata
     1801 cagcatagcc gggtatgtag cgcttaatcg gcggcgggtc aacatccggg atgtttacaa
     1861 cgttgacgag ctcaactcct tcgatccgcc ggtctatttt ttcagggctg tggacaggtc
     1921 cacggggtac aagaccaagc aagtactggc agcccctatt ttcgacggcg ctgattccaa
     1981 cgaggtgata ggtgtagtgc agcttatcaa caccctgtcc ggaaatccgt tccaaaagag
     2041 cgccgaggag agagtcgtgg aactctgcaa ggccttggcc gtcggcttcc gccagctgga
     2101 aaagcccagg caggttggca ccaagaccaa atacgaccat ctggtcatag atggcgtggt
     2161 cactgcggcc gagctggaac tcgcaatccg tctcgcgcgc gggaagggca tcaatatcga
     2221 gcaggtactg aaggacgagt tcaaagttac cgattcggcg ctcggcctcg ccctgtcgaa
     2281 gtttttcggg ttgccatatg agccatttga tcccgatcgg gccaagccga ccgctctgct
     2341 gagaaaactc aagcgcacgt tcgtgaacag ccaggcgtgg gtgccgatca cggaagacga
     2401 ccagggcatg gtcattgtta cgaccgaccc tgagcgcgtc atcagctcgc gcattgtcag
     2461 ccaggtgttc ccccggaaaa aggttactta ccgcgtctgc agcaatagcg agtttgccgc
     2521 gaccgtagag catttctaca ggctggaggg cgctcctgcc tcagttgaca cgatcatcaa
     2581 aagaatggac caggatacct cggaagcacg cggcgtcgtt gtcgaggaaa aaaccgagcc
     2641 ggatagcgcg accgtgcagt tggtcaacaa gattatcgtg gaagcccacc agatgcgcgc
     2701 ttcggacatc catgtcgagc cgcgtcaggg cggcgtgaaa agcaagattc gattccgtat
     2761 cgacggttcg ctggtcgact acaccgatat tcccgcgagc caccacgccc aggtcgtcgc
     2821 gcggctgaaa atcatgtccg atctggacat tgcaaacaag agagaaccgc aagacggcaa
     2881 gatcgcattc aagcaatttg ttccgtcgct ggacatcgaa ttgcgcgttg caaccatccc
     2941 gacagtcggc gggaaggaag atgtggtcat gcgcatcctg acggccggcc ggccggtgcc
     3001 gctcgaacaa ctgggtctca gccagcacaa tttgcggatc ttgaaatcac tgatatccaa
     3061 gccttacggc ttgttcctgg tatgcggtcc gaccggctcg ggcaagacga ccactttgca
     3121 ttcggttctc ggccacatca acaccccgga aaccaagatc tggaccgccg aagatcccgt
     3181 tgaaatcacc caggagtctc tgcggcaggt gcagatcaac ccgagggccg acctgacatt
     3241 tgcccgcgcg atgcgcgctt tcctgcgcgc cgatccggac gtgatcatgg tgggcgagat
     3301 gcgcgacagg gaaaccgccg ccatcggcat cgaggcctcg ctgaccgggc accgggttct
     3361 ggcgaccatt cacaccaaca gcgcgccgga gagcatcatc cgcctgctcg acatgggcat
     3421 ggacccgttc aacttcgctg acgcgttgat gggcatcatg gcgcagcgtc tggcaaagcg
     3481 cctgtgcgcc aagtgcaaga aaccgcacgt ggccacactg gaggagatcg gactgatgct
     3541 cacggaatac aaccaggacc tgaaagacac cgacaggttc aagtccgacg ccaaagcggc
     3601 gtacgaggcg gttcacgccg agtggacgaa gaactatgcc tatgacaaag ggcagttcat
     3661 tctctatgaa ccggtagggt gtgaagcgtg ccggggcacc ggctatagcg gacgggtcgc
     3721 cctgcacgag ttgctggcgg tcaccgatgc gatcaagaaa aatatccagg aacacgcgcg
     3781 agtggcggag gttctt
//
LOCUS       DBNGWZ010000003         2215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2015382, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000003 DBNGWZ010000000
VERSION     DBNGWZ010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2015382"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16CX_00030"
     CDS             complement(<1..815)
                     /locus_tag="AC16CX_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3010933.1"
                     /translation="MNLPGTSYRLRIETRSDNPGWMGLVSSVLGVVLALIVGGFVLRA
                     AGANDPIATYREIFKEGFGTPADWQAGITALLASGDCPKGQLCFGPLSDTLVKASPIL
                     LTGLACIVAFRMKLWNIGADGQMFLGAWAATGVAVFLLPKTTDRAVMLGAMALAGSLA
                     GLAYGAIPGFLKARLNINEIITTLMLNYVAYKWVEFFVVVGPWSIGDFESTGRFPTAA
                     TWPRLTEYARQIPIAAGLTAHPGILLGIGAAVLLAWILYRSRWGYEIRVIGNNP"
     gene            complement(812..>2215)
                     /locus_tag="AC16CX_00035"
     CDS             complement(812..>2215)
                     /locus_tag="AC16CX_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3010934.1"
                     /translation="ENGAGKSTLMNVLAGLYRPEAGEIFVGDQRVSFRSPQDAIAHGI
                     GMIHQHFMLVTSHSVAENMVLGLDRPRFFLDLPRVEEQVTALGAQYGLSVNPRARIWE
                     LSVGEQQRVEVLKMLFRGAKVLIMDEPTAVLTPQEVEALFHTLRDMTKRGHTIVFISH
                     KLDEVLAIADRVTVLRRGTVTAAGVPAKGATKAQLARLMVGREVLFLLEKKEAQPGEA
                     MLTVERLCADNDRGLPALREVSLQVRAGEILGIAGVAGNGQRELAEVLTGLHRATSGS
                     MMFGNVALDSHANWLEIGQQLAHIPEDRAAVGSVPGLSVAENLILKRFNRPPFAVGWS
                     INAAAVRQNAVRLVEKYKIATPTVEMPVRMLSGGNLQKLILARETSEHPKLMIAVHPT
                     RGLDVGAIEAIHRLLIEHRDRGAAILLISEELDELVSLADRIAVMYEGRIVGEMPAEG
                     ADIHAIGLMMAGAKVEA"
BASE COUNT          401 a          742 c          691 g          381 t
ORIGIN      
        1 ggattgttgc caatgacgcg gatctcataa ccccagcggc tgcgatacag aatccaggcc
       61 agcagaaccg ccgcgccgat gcccagcagg atgcccggat gcgcggtgag gcccgccgcg
      121 atggggattt gccgggcata ttcggtcagg cgcggccagg tcgccgccgt agggaagcgg
      181 ccggtggact cgaagtcgcc gatgctccag ggcccgacga cgacgaagaa ttccacccac
      241 ttgtaggcga cgtaattcag catcaaggtc gtaatgattt cgttgatatt gagccgcgct
      301 ttgagaaaac cggggattgc cccgtacgcg aggcccgcca agctccccgc cagggccatc
      361 gcccccaaca tgaccgcgcg gtcggtcgtc ttgggcaaga ggaacacggc gacgcccgtc
      421 gccgcccacg caccgaggaa catctggccg tccgcgccga tgttccacag cttcatccga
      481 aacgccacga tgcaggcgag gcctgtgagc aaaatcggcg aggctttgac cagcgtgtct
      541 gagagcgggc caaaacacag ctgccctttc gggcagtcac cgctcgcgag caaggcggtg
      601 atcccggcct gccagtcggc cggcgtgccg aagccttcct tgaagatttc tcgataggtc
      661 gcgatcggat cgttcgcacc cgccgctcgc agcacaaagc cgccgacgat cagcgccagc
      721 accacgccga ggacggacga aaccaggccc atccagcccg ggttgtcgga ccgggtttcg
      781 attctcaggc gataggaggt cccgggcaga ttcatgcttc caccttcgcg ccggccatca
      841 tcaggccgat ggcgtggatg tccgcgccct cagccggcat ttcgccgacg atccgcccct
      901 cgtacatcac ggcaatgcga tcggcgagcg agacgagctc gtccagctct tcggaaatca
      961 gcaggatggc cgcgccgcga tcgcgatgct cgatcaggag gcggtggatc gcctcgatag
     1021 cccccacatc cagtccgcgc gtggggtgaa cggcaatcat cagtttggga tgctcggagg
     1081 tctcgcgcgc gaggatcagc ttttgcaaat tgccgccgga cagcatgcgc acgggcatct
     1141 cgaccgtcgg cgtcgcaatc ttgtattttt ctaccagtcg cacggcgttc tggcgcaccg
     1201 ccgccgcgtt gatggaccag cccaccgcaa agggcgggcg gttaaagcgt ttgaggatca
     1261 ggttctcggc cacgctgagg cccggcacgc tgcccaccgc ggcgcggtct tcggggatgt
     1321 gcgccaactg ttggccgatc tccagccaat tggcgtgact gtcgagcgcc acatttccaa
     1381 acatcataga accgctggtg gcacggtgca gtccggtgag cacctccgcc agttcccgtt
     1441 ggccgttgcc cgccacgccg gcgatgccca gaatctcgcc ggcccggacc tgcagggaga
     1501 cctcgcgcaa cgcgggcagg ccgcgatcgt tgtcggcgca caggcgctcg accgtcagca
     1561 tcgcctcgcc tggctgcgct tccttctttt ccaacaagaa caagacttcc cggccgacca
     1621 tcagcctggc gagctgcgct ttggtcgccc ccttggccgg cacgccggcc gccgtcaccg
     1681 tgccgcgccg cagcacggtg acgcggtcgg cgatggcgag gacttcgtcg agtttgtgcg
     1741 agatgaagac aatagtgtgc ccgcgtttcg tcatatcgcg cagcgtgtgg aagagcgctt
     1801 cgacctcctg cggcgtcaag accgcggtcg gctcgtccat gatcaggact ttggcgccgc
     1861 gaaacagcat cttaagcact tcgacccgct gctgttcgcc caccgaaagc tcccagattc
     1921 tggcgcgggg gttcacgctc aggccgtatt gcgccccgag cgccgtgacc tgttcctcga
     1981 cgcgcggcag gtcaagaaaa aaacgcgggc ggtcgagtcc caacaccatg ttctccgcca
     2041 ccgagtgcga ggtcacgagc ataaaatgct ggtggatcat gccgatgccg tgcgcgatcg
     2101 catcttgcgg cgagcggaag gaaacccgct ggtcgccgac gaagatttca ccggcttctg
     2161 ggcggtacag gcccgccagc acgttcatca acgtgctctt gcccgcgccg ttctc
//
LOCUS       DBNGWZ010000004         2873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2015389, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000004 DBNGWZ010000000
VERSION     DBNGWZ010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2015389"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16CX_00040"
     CDS             complement(<1..203)
                     /locus_tag="AC16CX_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HIR3010935.1"
                     /translation="MPPVEPTLAALPNPVLRYFLATRPAFLSVTLFACLVGTGTAWYD
                     GVVLSVAKAVTTLVFALLAHAGI"
     gene            complement(339..908)
                     /locus_tag="AC16CX_00045"
     CDS             complement(339..908)
                     /locus_tag="AC16CX_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR3010936.1"
                     /translation="MALSEERHAMHDSYIFLDEKCLLLAEAMMRELRRYDELVASLRA
                     MLKEAAAVLHAALARHGLQGLQCYPPADLRDAAPRVTRQRLMGVVLQTAELEIADRPA
                     LEPVNPSPEAEACREQYAQAAALAARIAAVSGNLERLYHEYRRSVRRVRALQDVLLPE
                     IDRTVYEIETRLEELEQDEALWLRHHRGT"
     gene            complement(883..975)
                     /locus_tag="AC16CX_00050"
                     /pseudo
     CDS             complement(883..975)
                     /locus_tag="AC16CX_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(938..2320)
                     /locus_tag="AC16CX_00055"
     CDS             complement(938..2320)
                     /locus_tag="AC16CX_00055"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR3010937.1"
                     /translation="MYARVMVQGAAARIEGPLLFLRRTLEVGLNEAVEVRGADGRPRL
                     GRIAALDEQLVTVEVLESTSGLALPDVTARFLGEPLHFTLGPGLLGRVFNGIGQVIDG
                     GPPLAAHRRMRIDGLPFNPVARETPRDFIETGITAIDLMNSLVRGQKLPIFSGGGLPH
                     DRMAAEIASYARLRGARPEDFAIVFAGIGLPYERAEYFRRSLEAGGGLERAALFLNLA
                     SDSSSQRLLTPRYALTAAEYLAFVEGKHVLVILTDMTNYCEALREVAGAKGEIPGRKG
                     YPGYLYSDLAAIYERAGCLRGARGTLTQLPILSMPSDDIGHPIPDLSGYITEGQIVLS
                     RELERGGVHPPVNVLASLSRLMKNGTGKGYTHPDHPALASQLFAAYARAVQTRLLASV
                     VGEEGLSEADRAYLAFGTAFERELVSQDGPRLLEESMEAGWAVLRRLPRAELTRLTDA
                     QITEHIGRAP"
     gene            complement(2310..>2873)
                     /locus_tag="AC16CX_00060"
     CDS             complement(2310..>2873)
                     /locus_tag="AC16CX_00060"
                     /inference="COORDINATES: protein motif:HMM:NF047300.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase beta subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR3010938.1"
                     /translation="LKRAQARFYPAIHPLQSYSEDAGELAEWWERIFGAVTAGAVAGH
                     PASEWLSLRRRFLTLLDEQVRLERMARIVGKDALPPRQQLALFCAEIVNEAFLRQSAF
                     SEVDWYASPQRQAAMMRLIGRFIALAEQALAQGVLLERLAALGFLRQLQRMGEEIGED
                     RLGDFDTLAQCIEETFRALPGESGHVR"
BASE COUNT          458 a         1014 c          954 g          447 t
ORIGIN      
        1 ttgatgccgg cgtgcgcgag gagcgcaaac accagggtcg tgaccgcttt cgccactgac
       61 aggacgactc cgtcgtacca tgcggttcct gtcccaacca ggcaggcgaa cagcgtcacg
      121 ctcaggaatg ccggccgcgt cgcgagaaag tagcgcagta ccggattggg aagcgcggcc
      181 agcgtcggtt ctacaggcgg cattaggcca tctcctcgtg ccgttccaac ttgtctcgcc
      241 aaatactggt cgagcgcccg ctcaatacag ccggccattg gagggtcagt tggcttggca
      301 taaatagttg gaacggcact cgtcgggggc gcagtctctc aagtgccgcg atgatgcctg
      361 agccagagcg cctcgtcctg ctccagctcc tcgagccggg tctcgatctc gtagaccgtg
      421 cggtcaatct ccggcaacag cacgtcctgc agcgcgcgca cccgccggac cgagcgccgg
      481 tactcgtgat agaggcgctc gaggttgccc gaaacggcgg cgatccgggc ggccagcgcg
      541 gccgcctgcg cgtactgctc ccggcacgcc tcggcctccg gcgaggggtt gaccggctcg
      601 agcgccggac gatctgcgat ctcgagttcc gccgtctgca gtacgacgcc catcaagcgc
      661 tgccgcgtga ccctgggagc cgcgtcgcgg agatccgcgg gcggatagca ctgcaagccc
      721 tggagcccgt gccgtgcaag cgcagcgtgg agcacggcgg cggcttcttt gagcatcgcg
      781 cgcagggacg ccaccagctc atcatagcgt ctcagctccc tcatcatcgc ctcggcgagc
      841 agcaggcatt tctcgtcgag gaagatgtag ctgtcgtgca ttgcgtgccg ctcttcgctg
      901 agcgcgatga cggcgctgcg ggtcggcgtg atctcgctca cggcgcgcgc ccgatgtgtt
      961 cggtgatctg ggcatccgtg agccgcgtga gctcggcccg tggcagccgg cgcagcacgg
     1021 cccatccggc ttccatgctc tcctcgagca agcgcggccc gtcctggctc accagctcgc
     1081 gctcgaatgc ggtaccgaat gcgagatagg cgcgatccgc ttcggacagc ccctcctccc
     1141 ccaccacgct cgccagcagc cgggtctgca ccgcgcgcgc gtaggctgcg aagagctggc
     1201 tcgccagcgc cgggtggtcc ggatgcgtgt agcccttgcc ggtgccgttc ttcatcagcc
     1261 gcgacaggct ggcaagcacg ttgaccggcg gatggacgcc tccacgctcg agctcgcgtg
     1321 acagcacgat ctggccctcg gtgatgtagc cggacaggtc gggaatggga tgcccgatgt
     1381 cgtccgaggg catgctgagt atcggcagct gagtgagcgt gccgcgcgcg ccgcgcaggc
     1441 agcccgcgcg ctcgtagatc gccgcgaggt ccgagtagag gtagccggga tagcccttgc
     1501 gcccggggat ctcgcccttc gcccccgcca cctcgcgcag cgcctcgcag tagttggtca
     1561 tgtcggtgag gatgaccagc acgtgcttgc cctcgacgaa agccagatac tcggccgcgg
     1621 tcagcgcgta gcgcggcgtg agcaagcgct ggctgctgga atcgctcgcc aggttgagga
     1681 acaacgccgc gcgctcgagc cctccgcccg cctcgaggct gcgccggaaa tattccgcgc
     1741 gctcgtacgg caggccgatg ccggcgaaca cgatggcaaa gtcctcgggc cgcgcgccgc
     1801 gcaggcgggc gtaggaagcg atctctgcgg ccatgcggtc gtgcggcagg ccgcctccgg
     1861 aaaaaatcgg cagcttctgc ccccgcacca ggctgttcat caggtcgatc gcggtgatgc
     1921 cggtctcgat gaaatcgcgc ggcgtctccc gcgccaccgg attgaacggc agcccgtcga
     1981 tccgcatgcg gcgatgcgcg gcaagtggcg gcccgccatc gatcacctgg ccgatcccgt
     2041 tgaacacgcg gccgagcagg cccgggccca gcgtgaaatg cagcggctcg ccgaggaagc
     2101 gcgcggtcac gtccggcagc gcaaggcctg acgtcgactc cagcacttcc accgtcacca
     2161 gttgctcgtc cagggccgcg atccgcccga gccgcggacg gccgtccgcg ccgcgcactt
     2221 ccaccgcctc gttcaatccc acctcgagcg tgcgccgcag gaacagcaac ggaccttcga
     2281 tgcgcgcggc cgcgccctgc accatgaccc tagcgtacat gcccgctctc ccccggcagc
     2341 gcgcggaacg tctcctcgat gcactgcgca agcgtgtcga aatccccgag ccggtcctcg
     2401 ccgatttcct cgcccatgcg ctgcaactgc cgcaaaaatc cgagcgcggc gaggcgttcg
     2461 agcagcacgc cttgcgcgag cgcctgctcc gcgagggcga tgaagcgccc gatcaggcgc
     2521 atcatcgcgg cctggcgctg cgggcttgca taccagtcga cctccgaaaa agccgattgc
     2581 cgcaggaacg cctcgttcac gatttccgcg cagaacagcg cgagctgctg gcgcggcggc
     2641 agcgcgtcct tgccgacgat gcgcgccatg cgttcgagcc ggacctgctc gtcgagcagg
     2701 gtcagaaacc ggcggcggag actcagccac tcgctggcgg gatggccggc gaccgctccg
     2761 gcggtgaccg cgccgaagat cctttcccac cattccgcaa gctccccggc gtcctcggag
     2821 tacgactgca gcgggtggat cgcgggatag aagcgggcct gggcgcgctt cag
//
LOCUS       DBNGWZ010000005         2511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3023035, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000005 DBNGWZ010000000
VERSION     DBNGWZ010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3023035"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="AC16CX_00065"
     CDS             <1..1322
                     /locus_tag="AC16CX_00065"
                     /inference="COORDINATES: protein motif:HMM:NF051647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SelB domain-containing protein"
                     /protein_id="HIR3010939.1"
                     /translation="PAQRAQAGQRCALNLTGADLETVARGDWLLHEAIHAPTQRIDAR
                     ITVLASETRPLEHWTPLHVHLATADVTGRVSIPGAAAVPPGGSAIVQLALNKAVGALN
                     GDRFILRDQSARRTLGGGVVLDPFARPARRASGARLAELAALEQGSPEAALAEMLKNP
                     GRGVDLARFERTFNLKPERAAALYAAAGAAVLGKEVRTAVTRTFRDSLREQLLATVRD
                     FHRNQPQAVGMEPGALRKQLAPQLPEAAFQSLVRELADERKLEIASNLVRQPGHDATS
                     NPEDQRMWQLVLPALLQGGFSPPPVAELAAALKLKEATLKDFLHRKSRGGEVMRIPPD
                     RFYPRATLATLAATANAVAQSSPNGAFTAAQYRDATGIGRSLAIEILEFLDTLGITQR
                     IGDARKMRKDFSAILGPAEPARAPAKAAVPKPAAPAKPRPSPSPNRR"
     gene            1485..2390
                     /locus_tag="AC16CX_00070"
     CDS             1485..2390
                     /locus_tag="AC16CX_00070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3010940.1"
                     /translation="MTQNYDLIVIGEGISGLTCAGHARRAGLSVATFESNLFGGLVLN
                     VNELEGYPGAESGTNLASELMQANSEAGVASIQEEVTAIRAGGGMFEVNTADSGYGTR
                     QVVIASGARLKKLGVPGEDEFEGRGVSHCADCDAPMFQNEEVVVVGGGDSALQEALVL
                     AKFCHKVHVVHRGDRFRARRHFVDRIGANPRISTIWNTTVEAILGGKMVEKVRVKREG
                     KTEEITAAGVFAYVGLAPNSEIAPPGAARDEGDHLVTNDEFETAVAGLWAIGAVRAGY
                     SGLLRDAAAEAQNAAEGIVKRLRFD"
BASE COUNT          459 a          838 c          826 g          388 t
ORIGIN      
        1 agccggcgca gcgggcgcag gccgggcagc gctgtgcatt gaatctcacc ggcgccgatc
       61 tggaaaccgt cgcgcgcggc gactggctgc ttcacgaggc gatccacgcg ccgacgcagc
      121 ggatcgatgc gcgcatcacc gtgctggctt ccgaaacgcg gccgctcgag cactggacgc
      181 cgttgcacgt gcacctcgcc accgcggacg tcaccggccg cgtctcgatt cccggggcgg
      241 ccgcggttcc tcccggcggc tccgccatcg tgcagctcgc gctgaacaag gcggtgggag
      301 cgctcaacgg cgaccgcttc atcctgcgcg atcaatccgc gcgccgcacg ctcggcggcg
      361 gcgtggtgct cgacccgttc gcccgcccgg cgcggcgcgc ttcgggcgcg cggctggcgg
      421 agcttgcagc gctcgagcag ggctcgcccg aggcggcgct cgccgaaatg ctgaaaaacc
      481 cgggccgggg cgtggatctc gcccgcttcg agcgaacgtt caacctcaag ccggagcgcg
      541 ccgccgccct ctacgctgcc gcaggcgccg ccgtcctcgg caaggaggtc cgcaccgccg
      601 tcacgcgcac ttttcgcgac agcctgcgtg aacagttgct cgcaaccgtg cgggacttcc
      661 atcgcaacca gccccaggcg gtagggatgg aacccggcgc gctgcgcaaa cagctcgcgc
      721 cgcagctgcc cgaagcggca tttcagtcgc tcgtgcggga gctcgccgac gagcgtaagc
      781 tggaaatcgc gagcaacctc gtgcgccagc cgggtcacga tgccacttcc aaccccgaag
      841 accagagaat gtggcagctc gtcctgcccg cgttgctgca gggcggtttc tcgccgcccc
      901 cggtggcgga gctcgccgcc gcgctcaagc tcaaggaagc gacgctcaag gacttcctgc
      961 accgcaagag ccggggcggc gaagtgatga ggatccctcc ggaccgtttc tacccgcggg
     1021 cgacgctcgc cacgctcgcc gcgaccgcga atgcagtcgc acaatcgagc ccgaacggcg
     1081 cgttcaccgc ggcgcagtac cgcgacgcca ccggcatcgg caggagcctc gcgatcgaga
     1141 tcctggagtt cctcgacacc ctcggcatca cgcagcgcat cggcgacgcg cgcaagatgc
     1201 gaaaagactt cagtgcgatc ctgggaccgg ctgaaccggc gcgcgcgccc gcgaaagccg
     1261 cagtgccgaa gcctgcggct cccgcgaaac cgcgaccgtc accttctcct aatagacgat
     1321 gattgaccaa atactgccaa gatcaacacc agaatggaca ggattaacag gattcacaag
     1381 attaaacccc aattcaaaat ccaagccggg ttttgccttt tcttgttttt cgatctaatc
     1441 ctgtaaatcc tgttaatcct gtctatttca tccggtattt gaccatgaca cagaactacg
     1501 acctaatcgt gatcggcgag ggcatcagcg gcctcacctg cgccgggcac gcgcgccgcg
     1561 cggggctcag cgtggcgaca ttcgaatcca acctgttcgg cgggctggtg ctgaacgtga
     1621 acgagctcga gggttatccc ggggcggaat ccggtaccaa cctcgcttcc gaactgatgc
     1681 aggccaattc ggaagcgggc gtggcgagca tccaggagga agtcaccgcc atccgcgccg
     1741 gcggcgggat gttcgaagta aacaccgcgg acagcggcta tgggacgcgc caggtcgtga
     1801 tcgcgtcagg cgcgcggctg aagaagctgg gtgtgcccgg cgaggacgaa ttcgaggggc
     1861 gcggcgtctc gcactgcgcg gactgcgacg cacccatgtt ccagaatgaa gaggtggtgg
     1921 tggtcggcgg gggggactcc gcgctgcaag aggcgctggt gctggcgaag ttctgccaca
     1981 aggtccatgt cgtccaccgc ggcgatcgct tccgcgcgcg acggcacttc gtggatcgga
     2041 tcggcgccaa tcctcgtata tccaccatct ggaacaccac ggtcgaggcg atcctcggcg
     2101 gtaaaatggt cgagaaggtg cgcgtgaagc gcgaagggaa aacggaggaa atcacggccg
     2161 ccggggtatt cgcctacgtc gggctcgcgc cgaactccga gatcgcgccg cccggggccg
     2221 cgcgcgacga gggcgatcat ctcgtcacca acgacgaatt cgagaccgcg gtggcgggcc
     2281 tgtgggcgat cggggcggta cgcgccggct acagcgggct gctgcgcgat gccgctgcag
     2341 aggcacagaa cgccgccgaa ggcatcgtca aacgcctgcg gtttgattga tgcctcggag
     2401 cagcggcata atattgccga cctaaagtat tttttattaa gacgcgctcg cctggagcgc
     2461 gtcttcacgg aagggaatcg tccccggtgg ggcggccgga cttcaaaccc g
//
LOCUS       DBNGWZ010000006         4966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_432, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000006 DBNGWZ010000000
VERSION     DBNGWZ010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_432"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC16CX_00075"
     CDS             complement(<1..522)
                     /locus_tag="AC16CX_00075"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3010941.1"
                     /translation="MDRSFTVAQKIRFAHCDPEGILYFPHVFDFVNTAVEEWFESGLG
                     MPFDAFHMEHGLGNPVVTTQCEFLRPCRFGEVLKLELAPTRIGRSSTEFHVAVKVGED
                     ERMRLHHRTAMISMDSFRAIAIPDALRARMETFLVPSMGRSHRSALPARTDATPANAF
                     RSRQLVRYAHCDPG"
     gene            606..1064
                     /locus_tag="AC16CX_00080"
     CDS             606..1064
                     /locus_tag="AC16CX_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3010942.1"
                     /translation="MSAGLRSRIEAFLQAHHVMSLATSGPEGSWSAAVFYAADGLTLY
                     FISAPGSRHSRDIEASGRAAATIHRDVGDWREVRGIQLEGEAGRVGGTERAAALACYG
                     RKFPLIADLASAPPEIAAAMARVEWYRLSPRRVRLVDNTLGFGHREELDL"
     gene            complement(1061..1303)
                     /locus_tag="AC16CX_00085"
     CDS             complement(1061..1303)
                     /locus_tag="AC16CX_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR3010943.1"
                     /translation="MALTINEDCTLCDACVPVCPNEAITKGEPYIIDPLKCTECVGAE
                     DEPQCKLVCPSNCILEHPDFRESRDELLAKYHSLHG"
     gene            complement(1318..2157)
                     /gene="bcrD"
                     /locus_tag="AC16CX_00090"
     CDS             complement(1318..2157)
                     /gene="bcrD"
                     /locus_tag="AC16CX_00090"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HIR3010944.1"
                     /translation="MTFTVGIDVGSGVVKTVLFRCDGGSVEWLARRAERIRRRDPLQL
                     ARAGRDEVLEEAKLKAEDVDYVATTGEGENVKFATGHFYSMTTHARGGIFLNPQARAV
                     VDVGALNGRAIRIDERGKVISYKMTSQCASGSGQFLENIARYLGVAVEEIGPLSQSSK
                     NPEKVSSICAVLAETDVINMVSRGIATPDILKGIHLSMASRIVKLLKVTGIREGTALV
                     TGGLALDSGLIAAMGEEMVNEKLNLAAVSHPDSIYAGAIGAAIWGAFRHDKLARLQER
                     AAA"
     gene            complement(2154..3497)
                     /gene="bcrA"
                     /locus_tag="AC16CX_00095"
     CDS             complement(2154..3497)
                     /gene="bcrA"
                     /locus_tag="AC16CX_00095"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit A"
                     /protein_id="HIR3010945.1"
                     /translation="MRCFIGIDLGSTTTKAVVIDERREVLGRGITNSRSNYDTAAAVA
                     KQEALVSARFELFRQALAASGALNGKLDEFIGQLERDFRLEQFLEQLADLEATCQRNA
                     AGTRFGDSAGGVAQALAELFRRLRDDSPALFIPNAKRKSDFFRDIAGAQYLALGEAVA
                     KDAGVRYDMLLNVFDRSIIEVENREYGDAISRNLLAAMDRTLKAMPEAAARSERIRAP
                     IQGVLDTALEETYVVGTGYGRARLPFSKEHIRSEILCHGLGAHVMYPGTATVLDIGGQ
                     DTKAIQVDPHGIVESFQMNDRCAAGCGRYLGYIADEMNMGLHELGPLAMKSTRTIKIN
                     STCTVFAGAELRDRLALGDKREDIMAGLHRAIVLRAMSILARSGGIRNEFTFTGGVAK
                     NEAAVKALKALVSENYGALTLNINPDSIYTGALGGATFAWRAVVEEGRTAEARAK"
     gene            complement(3511..4833)
                     /gene="bcrB"
                     /locus_tag="AC16CX_00100"
     CDS             complement(3511..4833)
                     /gene="bcrB"
                     /locus_tag="AC16CX_00100"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HIR3010946.1"
                     /translation="MSTPIASPASKKPAAPVQKEDAMLLQKEMINRNYHDLAVAHDCG
                     RKISATFVPGNLNELLMCFDFARSLPETNALQNALRKRTGKMIMDAERNGHSEDVCTY
                     VKADLGMMLAGDTGPTGEPLPKPDVLLLSYTGCFTFMKWFELIRQKYGGEMIMLHVPY
                     QGDGRIAPNMRDYVVKQIKDSVIPTLERVSGVKFDIDRLREYMRESQKAEENLVRVLQ
                     AGKHRPSPIDGYFGGVYYIGPMFTAFRGSPEATRYYDVLWSEVENRMREGRGPITPEG
                     EITEEKFRLVVEGPPNWTNFRDFWKMFYDEGAVVVASTYSKVGGVYDFGFRHDPERPL
                     ESLAEYCLGCYTNLNFPQRIDMICRYLEDYQADGLLINSIKSCNSFSAGQLLILREVE
                     KRTGKPAAFIESDLVDPRYFSAANIRNRLESYFQMIKQKRAAQAATVH"
BASE COUNT          801 a         1613 c         1644 g          908 t
ORIGIN      
        1 cccgggatca cagtgcgcgt atcgcaccag ctgccgggag cggaaggcgt tcgccggcgt
       61 cgcgtccgtc cgggccggca gcgcggaacg gtgagacctg cccatcgaag gaacgagaaa
      121 agtctccatt cgcgcgcgca gcgcatcggg aatcgcgatc gcgcggaagc tgtccatcga
      181 gatcatcgca gtgcggtggt ggagccgcat gcgctcatcc tcccccacct ttacggcgac
      241 gtgaaactcg gtggaactgc gcccgattcg cgtgggcgcc agttccagct tcagcacctc
      301 gccgaaccgg cacggacgca ggaactcgca ctgggtggtg acgacgggat tgccgaggcc
      361 gtgctccatg tgaaacgcgt cgaacggcat gccgaggccg gattcgaacc attcctcgac
      421 cgccgtgttc acgaaatcga aaacatgcgg aaagtagaga atgccctccg gatcgcaatg
      481 cgcgaaacgg atcttctgcg caacggtaaa actcctgtcc atcccgtgta tgttacccaa
      541 agtgcgtttc cgataaagta aatgcctcac cctgaggtcc gaggcaaggg ccaggaggaa
      601 atgaaatgag tgccgggctg cgctcgcgca tcgaggcatt cctgcaggcg caccacgtga
      661 tgagcctcgc gacctcgggg ccggagggct cctggtcggc ggcggtgttc tacgcagccg
      721 atggcctgac gctctacttc atctcggcgc cgggaagccg gcacagccgc gacatcgagg
      781 cgagcggacg cgccgcggcg acgattcaca gggacgtcgg cgactggcgc gaagtccgcg
      841 ggatccaact ggagggcgag gcggggcgcg tcggcgggac cgagcgcgcc gcggcgctcg
      901 cgtgctacgg gcggaaattt ccgctgatcg cggacctcgc ctcggcgccc ccggaaattg
      961 cggccgccat ggcgcgcgtg gagtggtatc gcctgtcgcc gcggcgcgtg cggctggtgg
     1021 acaacacgct cggcttcggc caccgcgagg agctggatct ctagccgtgc aggctgtgat
     1081 acttcgccag caactcgtcg cggctctcgc ggaagtcggg atgctcgagg atgcagttcg
     1141 acggacagac cagcttgcac tgcggctcgt cctcggcgcc gacgcactcg gtgcacttca
     1201 gcggatcgat gatgtagggc tcgcccttgg tgatcgcctc gttcgggcac accggcacgc
     1261 aggcatcgca cagcgtgcag tcttcgttga tcgtcagagc catgtgattc tcccggttca
     1321 ggccgcggcg cgctcttgca gccgcgcgag cttgtcgtgg cggaaagcgc cccagatcgc
     1381 cgcgccgatc gcgccggcgt agatcgagtc cgggtggctg accgccgcca gattgagctt
     1441 ctcgttcacc atctcctcgc ccatcgcggc gatcaagccc gaatcgagcg cgagcccacc
     1501 ggtcaccagc gccgtgccct ccctgatgcc ggtcaccttg agcagcttca cgatgcgcga
     1561 tgccatcgag agatggatgc ccttgagaat gtccggcgtg gcgatcccgc gtgagaccat
     1621 attgatgacg tcggtttcgg ccagcaccgc gcagatcgag ctgactttct ccgggttctt
     1681 cgagctttgc gacagggggc cgatctcctc gaccgccacg ccgagatagc gcgcgatgtt
     1741 ttcgaggaac tgtcccgagc ctgaagcgca ctggctggtc atcttgtacg aaatcacctt
     1801 gccgcgctcg tcgatgcgga tcgcgcggcc gttcaacgcc ccgacgtcga cgacggcgcg
     1861 cgcctgcgga ttgaggaaaa tcccgccgcg cgcatgcgtg gtcatcgagt agaagtggcc
     1921 ggtggcgaat ttgacgtttt cgccctcgcc ggtggtcgcg acgtaatcga catcctccgc
     1981 cttgagcttc gcctcctcca gcacttcgtc tcggccggcg cgggcaagct gcaaggggtc
     2041 gcgccggcgg atgcgctcgg cgcgccgcgc gagccactcc acgctcccgc cgtcgcagcg
     2101 gaacagcaca gttttcacga cgcccgagcc gacgtcgatg ccgacggtga acgtcatttc
     2161 gccctcgcct ccgcggtcct gccctcctcg accaccgcgc gccatgcgaa ggtggcgccg
     2221 ccgagcgcgc cggtgtaaat cgagtccgga ttgatgttga gcgtcaatgc gccgtagttt
     2281 tcactgacga gcgccttcag cgccttcacc gccgcctcgt tcttcgccac gccgccggtg
     2341 aaggtgaact cgttgcggat gccgccggaa cgcgccagta tcgacatcgc gcgcaggacg
     2401 atcgcgcggt gaagtccggc catgatgtcc tcgcgcttgt cgccgagcgc cagccggtcg
     2461 cgcaattcgg cgccggcgaa caccgtgcat gtcgaattga tcttgatggt ccgggtcgat
     2521 ttcatcgcca ggggcccgag ctcgtgcagg cccatgttca tctcgtcggc gatataaccg
     2581 aggtaacggc cgcagcccgc ggcgcagcgg tcgttcatct ggaacgattc gacgatgccg
     2641 tgcgggtcga cctggatcgc cttggtgtcc tggccgccga tgtcgagcac ggtggcggtg
     2701 cccggataca tcacgtgcgc gccgaggccg tggcacagga tctcggagcg gatgtgctcc
     2761 ttggagaacg gcagccgcgc gcggccgtag ccggtgccga ccacgtaggt ctcctccagc
     2821 gccgtgtcga gcacgccctg gatcggcgcg cggatcctct cgctgcgcgc ggcggcttcg
     2881 ggcatcgcct tgagggtgcg gtccatcgcg gcgagcaggt tgcggctgat cgcatcgccg
     2941 tactcgcggt tttccacctc gatgatcgag cggtcgaaca cgttgagcag catgtcgtag
     3001 cgcacgcccg cgtctttcgc gaccgcctcg cccagcgcga ggtactgggc gcccgcgatg
     3061 tcgcggaaga aatcgctctt gcgcttggcg ttgggaatga acagcgccgg cgagtcgtcg
     3121 cgcaggcgcc tgaacagctc ggcgagggcc tgcgccaccc cgccggcgga atcgccgaag
     3181 cgggtgcctg ccgcattccg ctggcaggtc gcttcgaggt cggcaagctg ctcgaggaac
     3241 tgctcgagac ggaaatcgcg ttcgagctgg ccgatgaatt cgtcgagctt gccgttcagc
     3301 gcgccgctcg ccgcgagggc ctggcggaac agctcgaagc gcgccgaaac cagcgcctcc
     3361 tgcttggcca ccgcggccgc ggtgtcgtaa ttggaccgcg agttggtgat gccgcggccc
     3421 agcacctcgc ggcgctcgtc gatgaccacc gccttggtgg tggtcgatcc caggtcaatg
     3481 ccgatgaagc agcgcatgtg cggccgccgt tcagtgcacg gtcgcggcct gggccgcgcg
     3541 cttctgcttg atcatctgga agtagctctc caggcggttg cggatattcg cggccgagaa
     3601 gtagcgcgga tcgaccaggt cggactcgat gaacgcggcc ggcttgccgg tgcgcttttc
     3661 cacttcgcgc aggatcagga gctgcccggc cgagaagctg ttgcagctct tgatcgagtt
     3721 gatcagcagg ccgtcggcct ggtagtcctc gaggtagcgg cagatcatgt cgatgcgctg
     3781 cgggaagttg agattggtat agcagccgag acagtactcg gcgagcgatt ccagcggccg
     3841 ttcggggtcg tggcggaagc cgaagtcgta gacgccgccg accttggagt aggtcgaggc
     3901 caccaccacc gcgccctcgt cgtagaacat tttccagaag tcgcggaagt tggtccagtt
     3961 cggcggcccc tccaccacca ggcggaattt ttcctcggtg atctcgccct cgggcgtgat
     4021 cgggcccctg ccttcgcgca tgcgattttc gacttcgctc cacaacacgt cgtagtagcg
     4081 ggtcgcctcc ggactgccgc ggaatgccgt gaacatcggc ccgatgtagt agacgccgcc
     4141 gaaataaccg tcgatcggcg acggccggtg cttgcccgct tgcagcaccc tgaccaggtt
     4201 ctcctcggcc ttctgcgatt cgcgcatgta ctcccgcagg cggtcgatgt cgaacttgac
     4261 gccggagacg cgctccagcg tcggaatgac actgtccttg atctgcttta ccacgtagtc
     4321 gcgcatgttc ggcgcgatcc tgccgtcgcc ctggtagggc acgtgcagca tgatcatctc
     4381 gccgccgtac ttctgccgga tcagctcgaa ccacttcatg aacgtgaagc agccggtgta
     4441 ggaaagcagc agcacgtcgg gtttgggcaa cggttccccg gtggggcccg tgtcgccggc
     4501 gagcatcatg ccgagatccg ccttgacgta ggtgcagacg tcctccgagt ggccgttgcg
     4561 ctcggcgtcc atgatcatct ttccggtgcg cttgcgcagc gcgttctgca gcgcgttggt
     4621 ctcgggcagg ctgcgggcaa aatcgaagca catcagcagc tcgttcaggt tgcccggcac
     4681 gaaagtggcg gagatttttc gcccgcaatc atgcgcgacc gccaggtcgt ggtagttgcg
     4741 gttgatcatc tccttctgca gcagcatcgc gtcctctttc tggaccggcg ccgccggctt
     4801 tttggatgcg ggcgatgcga tcggggtact catgcggcac tccagagttt gatggaatcg
     4861 gcgaacgtgc ccgcctgctc gcgaatcggc gccatctggc cggtgttctc cgcgtacttg
     4921 aacgcggtgc aggggatgcc gtgctgcgcg agaacgtcga gcagca
//
LOCUS       DBNGWZ010000007         2689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3023088, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000007 DBNGWZ010000000
VERSION     DBNGWZ010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3023088"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          469 a          907 c          897 g          416 t
ORIGIN      
        1 cccagctcgc tgaaagacat cgagcgcagc atcctcgcct ccgacctggg gttgacgcca
       61 aacaacgacg gcaagctcat ccggctgaac atcccgccgc tcaccgagga gcgccggaaa
      121 gagctgatca aagtcgtcca tcaccggctg gaagaagcgc gcgtggcggt gcgcaacatc
      181 cgccgcgcgg cgcacgacga cctgcgtgaa ttcgagaaag agaaggtgat ctcggaagac
      241 gacctcacgc gcggcgagac cgacctgcag aagctcaccg accggtacat cgagaagatt
      301 gacgaacacg gcaagcgcaa ggaagcagaa atcaaagaaa tctgaatggc cgacactccc
      361 ctgtccccgc ccgaaaaact ccccacccac gtcgccatca ttatggacgg caacggccgc
      421 tgggccttgt cgcgcgggct gccgcggctg gccgggcacc gcgcgggcgt cgagaatctg
      481 cgccggatca tcactgcctg cgtcgagttc ggcatcccct acctgaccat ctacgccttc
      541 tcgaccgaga actggggccg cccgccggag gaagtgaagg ggctgctgtt catcctggac
      601 gaggtgctgg accgcgagtt ggctgagctg cacaagcagg gcgtgcgcct ctggcacatc
      661 gggcggctgg agggcatcgc gccctccacg cagaagaagg tgcgcgacgc catcgagatg
      721 accaagaaca acacgcgcct cacgctcaac gtcgccttca attatggcgg gcgcgccgag
      781 atcgtccacg ccatccagca gatgctcgcc gacggcgtga ccaccggaca gatcaccgag
      841 gagctggtgg gccgctatct gtacacggcg ggccagcccg accccgatct catcatccgc
      901 acggccggcg agctacgcgt ctctaacttc ctcctctggc agggcgcgta tgccgagtat
      961 tacgccgccg acgtgtactg gcccgatttc aacaaagaag aattccggcg cgcgctggag
     1021 cacttcgcct cgcgcgagcg ccggttcgga ttggtgtctg tgaacccggc gggatagggg
     1081 acaacgcccc gctatgagac ggaaccgacg ctccgccgat gttcagcgac cccgctgatg
     1141 tttagcgacc gcatcgccgt ctcgatcttc ctgatcccgg ccgcgctgtg cgtggtcgcg
     1201 agcggcgggc cgatctatct gttggggatc ctgctcattt ttacgctggc cgcgctggaa
     1261 tacgcgcagt tgttccgcac ggggggacag cgccccgccc tcccgctcgt cgtcggcggc
     1321 gtgctggctc tcatcgccgc tcagcaattc cccgcgttga acccggacgg cctgttgttc
     1381 ggcctgatca tcctggctgc gctcacctgg caccagattg actacgagcg cggggcggcg
     1441 gcctcgggca ccgatctcgc catcaccgtc ggcggcatct tctacctcgg ctggctgggc
     1501 acgtacttca tcgccctgcg cgcgctgcgg ccggacgggc tgtggtggct gatcaccacc
     1561 gtgaccgccg tgtggctggc cgacacaggc gcatacacga ttggccgcgc tttcggcaag
     1621 cacccgatgg cgccgcgcct cagcccgaag aagacgtggg agggcttcgc gggcggcgtg
     1681 ctgggcggcg cgctcggcgc gggcgtgctg tcggtgttct ggcacctcgg cgcggcctac
     1741 gacgcgccgc aaacggcgct tacctggcag accggcgtgc tgctcggcgc gctcatcggc
     1801 gcactcagcc cgctcggcga cctgggcatc tcgatgctca agcggcagat cggggtgaag
     1861 gactccggcc atctcatcgc cgggcacggc ggcgcgctcg accgcatgga ctcgtggctg
     1921 gtggccgtgc cggtggggta ttattttgtg ctgattgtgc aaatgatgag gagataaacc
     1981 atgagcgaac gacccgaccg caacctggcc ctcgaactcg tgcgcgtgac ggaggccgcg
     2041 gcgctggccg ccgggcgttt catgggccgc ggcgataaag agggcggcga caaggccgcc
     2101 gtggacgcga tgcggctggt gctcaacacc gtcgagatgg acggcatcat cgtcatcggc
     2161 gagggcgtca aggacgaagc ccccatgctc tacaacggcg aggtgctcgg gacggggcac
     2221 gcccccagag tggacatcgc cgtggacccg attgacggga cgcgcctgct tgcgctcggg
     2281 cgcgccaact cgctctccac cgtcgccatc gcagaccgca gcacgatgtt cgaccccggg
     2341 ccgatcgtct acatggacaa ggtcgccgtc gggccgggcg ggaaggggac gattgacctc
     2401 gaagcctcac caaccgtgaa cctgcgcagc gtggcgcgcg cgatgggcaa ggacgtccgc
     2461 gacctggccg tgaccgtgct cgaccgcccg cggcacgaga agctcatcgc cgaagtccgc
     2521 aaggccgggg cgcgcatccg gctcatcacc gacggcgacg tagccggcgc gctcatgacg
     2581 gcctggccgg agtccggcat tgatttgctg atgggcatcg gcgggacgcc cgagggcgtg
     2641 atcgccgcgt gcgcgctcaa gtgcatgggc ggcgaaatcc agggcaagc
//
LOCUS       DBNGWZ010000008         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_378109, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000008 DBNGWZ010000000
VERSION     DBNGWZ010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_378109"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="AC16CX_00125"
     CDS             <1..524
                     /locus_tag="AC16CX_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta propeller repeat protein"
                     /protein_id="HIR3010947.1"
                     /translation="VEGFDPANLAVYDPHCVRLCPSNPDRLYQQNHCGIYRIDRPSNQ
                     WTRIGRSMPNRVGDIGFPMVVHPRDADTVWVFPMDGQTVWPRTSPEGMPGAYVTRNGG
                     KSWQRLDSGLPKSQAWWTVKRQAMTADAQDPVGLYLGTTSGELWMSRDEGNRWACIAR
                     NLPEIYAVEAAEA"
     gene            573..830
                     /locus_tag="AC16CX_00130"
     CDS             573..830
                     /locus_tag="AC16CX_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3010948.1"
                     /translation="MKVLIPSPLLSYTNEREVEATGATLAELLADLDRQYPGLRFRVI
                     DEQDKMRAHMRFFVNGEQVFDMTRSLRPTDAVQIVQALSGG"
     gene            934..1611
                     /locus_tag="AC16CX_00135"
     CDS             934..1611
                     /locus_tag="AC16CX_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3010949.1"
                     /translation="MVLPDWPHHVIQRGHNKQVVFRSDEDYAYYLQTLGEWKATLGCR
                     VYAYCLMTNHVHLLINPGPKAANLALLMKRVAGRQTRYRNARDGRTGTLWEGRYKSSP
                     IETEAYLLACGRYVELNPVRARMVKRPERYRWSSYRARAGLTEWGWLDRDPCYKGLGR
                     TAHERAARYRQWVKEAIPEGEWERIRHAIQRGQLTGGERFIHTVAKRVGRRVEFRGRG
                     RPRREQK"
     gene            1641..>1995
                     /locus_tag="AC16CX_00140"
     CDS             1641..>1995
                     /locus_tag="AC16CX_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3010950.1"
                     /translation="MVAGVMLVGAVSTVAAQPRAVEGPRVSLVDAVRLALETFPSVGA
                     SRAAGDEARAASGEAKASRLPTLTLDASLTQNQEPAPVTPIHGFAPGLTPQFDETLIQ
                     GGVSLQYRLFDGGVRS"
BASE COUNT          354 a          586 c          709 g          346 t
ORIGIN      
        1 tggtcgaggg cttcgacccg gccaatcttg ccgtctatga cccccattgc gtccggctct
       61 gtcccagcaa tccggaccgg ttgtatcaac agaatcactg cggcatctac cgaatcgatc
      121 ggccgtcgaa ccagtggacg cgtatcggca ggagcatgcc gaatcgggtc ggcgacatcg
      181 gctttccgat ggtggtgcac ccgcgcgatg cggacaccgt gtgggtattc ccgatggacg
      241 gtcagaccgt gtggccgcgc accagcccgg aaggcatgcc cggcgcctat gtcacgcgca
      301 atggcggaaa gagctggcag cgtctcgatt ccggcctgcc caagagtcag gcatggtgga
      361 cggtgaagcg ccaggccatg accgcggacg cgcaggatcc cgtgggattg tacttgggga
      421 cgacgagcgg agagctgtgg atgagccgcg acgaaggcaa tcggtgggcc tgcatcgcgc
      481 ggaatcttcc cgaaatctac gcggtggagg ctgccgaggc gtgatggcgc tgccgtgtct
      541 gcgtgggcga aggtcgcatc gagattgaga ccatgaaggt gctgattccc agcccgctgc
      601 tctcctacac caacgagcgg gaggtcgaag ccaccggcgc gacactcgcc gaactgctcg
      661 ccgacctcga ccgtcaatat cccggcctgc gattccgcgt gatcgacgag caggacaaga
      721 tgcgggcgca catgcggttc ttcgtcaacg gcgagcaggt gttcgatatg acgcgctcgc
      781 ttcgcccgac cgatgcggtg cagatcgtgc aggcgttgag cggcgggtga ggttcggtca
      841 gttcagcagc cgtggaaaaa ggggacagat ctattttctt gacttgctga gacgacgggg
      901 ggcagactgg cgggcatgcc gagacaaggg cgcgtggtgc tgccggactg gccgcatcac
      961 gtcatccagc gaggccacaa caaacaggtc gtttttcgat ccgacgagga ctacgcctac
     1021 tatttacaga ccctcgggga atggaaagcg accttgggtt gccgggtgta tgcctattgc
     1081 cttatgacca atcacgtcca tctgctcatc aacccggggc cgaaggcggc taacctggcc
     1141 ttgctgatga agcgggtggc ggggcggcag acgcgctacc gcaatgcccg ggatggacga
     1201 accggaacgc tgtgggaagg gcggtataag tcgagtccga tcgagacaga ggcgtatctg
     1261 ctggcctgtg ggcggtacgt ggaactcaat ccggtccggg cacggatggt gaaacggccg
     1321 gaacggtatc gctggtcgag ctatcgggca cgtgcggggc tgaccgagtg gggttggctg
     1381 gatcgcgatc cctgttacaa ggggttaggg cggacggcgc acgagcgggc ggcgcgctat
     1441 cggcagtggg tgaaggaggc gatcccggag ggcgaatggg agcggattcg gcacgccatc
     1501 cagcgcggcc agcttacagg cggcgagcgc tttattcaca cggtcgcgaa gcgagtcggg
     1561 cggcgggtcg agtttcgggg ccgagggcgg cccagaaggg agcaaaaata aatctgtccc
     1621 ctttttcccc ttttttcggg atggtggccg gcgtgatgct ggtcggagcg gtctcgacgg
     1681 tcgccgcgca gccgcgcgcc gtcgaaggtc cgcgcgtgtc gctcgtcgac gccgtccggc
     1741 tggcgctgga gacgtttccg tcggtcggcg cctcgcgggc cgctggggat gaggcccggg
     1801 cggcgagcgg ggaggccaag gcgtcgcggc tgccgacgtt gaccctcgac gcgtcgctca
     1861 ctcaaaatca ggagccggcc cccgtcaccc ccattcacgg cttcgctccg ggcctgaccc
     1921 cgcaattcga tgagacgctg atccagggcg gcgtcagcct gcaatatcga ctcttcgatg
     1981 gcggcgtccg ctcgg
//
LOCUS       DBNGWZ010000009         8593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3149065, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000009 DBNGWZ010000000
VERSION     DBNGWZ010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3149065"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC16CX_00145"
     CDS             <1..701
                     /locus_tag="AC16CX_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR3010951.1"
                     /translation="KGVHIVSADYSQIELRIMAHISQDASLLKAFASGEDIHRATAAE
                     IFGMAPEEVGAEQRRYAKVINFGLIYGMSAFGLARQLGLERSAAQQYMDRYFARYPGV
                     AEYMKRTRETARETGYVETVFGRRLWLPEIRGGNGARRQGAERAAINAPMQGTAADLI
                     KMAMIAVDRWLAEKRLSSSLIMQVHDELVLEVPDKELDRVRRELPKLMSGVTKLSVPL
                     VVDIGAGPNWDQAH"
     gene            complement(772..1452)
                     /locus_tag="AC16CX_00150"
     CDS             complement(772..1452)
                     /locus_tag="AC16CX_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR3010952.1"
                     /translation="MRHILGFFALLVISGHAPAAGLDRISNADAVSGLKQALNDGSLA
                     AVAKLGVENGYFGNPQVKIPLPPSLKRVEGAMRALGMRKQADELVLAMNRAAESAAPE
                     AKQLLVDAVKKMSVQDAKKILTGGDTAGTEYFKRTTRPQLTQRFLPIVKKATERVGLA
                     QKYNAIAGQGSSLGLVKEDQATIEQHVTQKALDGLYFMIGEQEKAFRKNPVGASSDIV
                     RKVFGALK"
     gene            1544..1999
                     /locus_tag="AC16CX_00155"
     CDS             1544..1999
                     /locus_tag="AC16CX_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3010953.1"
                     /translation="MLLLIAIFAGTTPAPQVTFVSLKGEKVTTADLKGRVVLVNFWAT
                     DCPICIKEMPDMVRTYERYRDSGFEFIAVAMKHDPPNYVIHYTENNRLPFKVALDPMG
                     DLARAFGDVKLTPTSFVIDKRGNIVLRVTGEPDFARLHELLEKKLGEAA"
     gene            complement(2010..2471)
                     /locus_tag="AC16CX_00160"
     CDS             complement(2010..2471)
                     /locus_tag="AC16CX_00160"
                     /inference="COORDINATES: protein motif:HMM:NF021425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HIR3010954.1"
                     /translation="MTRYTRGTRTVALLEGAKGVLVLAAGLGALSLIHRDVQAYAEEL
                     VRTFHLNPASHYPSIFLDAAGRLNDAEITLLASAAAGYAAARFVEAYGLWKERRWAEW
                     FAALAGAVYLPIEIVEIAHGLSWAKVTIFAINIAIVAYMSWVLWQSRQRTA"
     gene            complement(2517..3455)
                     /gene="lipA"
                     /locus_tag="AC16CX_00165"
     CDS             complement(2517..3455)
                     /gene="lipA"
                     /locus_tag="AC16CX_00165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3010955.1"
                     /translation="MSALGEKQKGRLKTARIPVKVVPAAPLAKPEWIRVRLGNGERFQ
                     EIKRILREHRLHTVCEEATCPNIGECFGKGTATFMILGDLCTRRCPFCDVAHGRPLPP
                     DPEEPFNLARTIAALKLGYVVVTSVDRDDLGDGGAKHFADCIRAVRELSPRTRIEVLV
                     PDFRGRLEIALEVLSAAPPDVMNHNLETVPRLYRQARPGADYWHSLKLLKDFKSRFPR
                     IPTKSGLMVGLGETDEEILSVMRDLRAHDVDMLTIGQYLRPSAHHLPVARYAEPAVFD
                     EFARQAHALGFMHAACGPLVRSSYHADRQAHGAGIV"
     gene            complement(3452..4108)
                     /gene="lipB"
                     /locus_tag="AC16CX_00170"
     CDS             complement(3452..4108)
                     /gene="lipB"
                     /locus_tag="AC16CX_00170"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3010956.1"
                     /translation="MTVVATDASVDGSRQASEVRVHRLGRVDYEPTWRAMQAFTSTRS
                     TNSADELWLLEHPPVYTLGVAGRAEHLPRAANGIPVIKTDRGGQITYHGPGQIVIYLL
                     LDLRRRGLAVRSLVRLMELAVIDLLAEYGIAAAGRTEAPGVYVDGAKIAALGLRVRNG
                     CCYHGLAFNVDMDLSPFHVIDPCGYSGLAVTQARDLGIRDDREILGGKLVRHLLDRLP
                     "
     gene            4185..4667
                     /locus_tag="AC16CX_00175"
     CDS             4185..4667
                     /locus_tag="AC16CX_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3010957.1"
                     /translation="MKKSFLILAVATVALAYGAALAAEASGDYATDLATVYGGYQRML
                     AMKEACDTAMPAARAANEKAFVAWRTQHRALIQDLQRRVTAMIRLASQDEKEYARNLG
                     KYEGAILQEREEYKEILLGLGQDELREQCQRMPEMLKGPGADLGKVYAAELETIRKRK
                     "
     gene            4840..4950
                     /locus_tag="AC16CX_00180"
     CDS             4840..4950
                     /locus_tag="AC16CX_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HIR3010958.1"
                     /translation="MGKGDKRTRRGKLFRGSYGKTRPGRKKKKKKPMAAR"
     gene            4972..5121
                     /locus_tag="AC16CX_00185"
     CDS             4972..5121
                     /locus_tag="AC16CX_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3010959.1"
                     /translation="MAQPQPNKLDSGDVFPKLDLMHTLAHGALALPGALAGKWGVILF
                     YRGHW"
     gene            5134..5466
                     /locus_tag="AC16CX_00190"
                     /pseudo
     CDS             5134..5466
                     /locus_tag="AC16CX_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(5549..5815)
                     /locus_tag="AC16CX_00195"
     CDS             complement(5549..5815)
                     /locus_tag="AC16CX_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP0495 family protein"
                     /protein_id="HIR3010960.1"
                     /translation="MIADTPLLEYPCDFPIKILGHTQAGFAQAVLEVVKRHAPDFDGA
                     TMELRASKRRKYLSVTCVIRATSREQLDGLYQELCDHPMVVMVL"
     gene            complement(5919..6779)
                     /locus_tag="AC16CX_00200"
     CDS             complement(5919..6779)
                     /locus_tag="AC16CX_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HIR3010961.1"
                     /translation="MSDIAYLNGEFLPLDQARVSVLDRGFIFGDGIYEVIPVYSRRPF
                     RLPEHLKRLQRSLDAIWLANPMTDAEWTQLVHDLVARHAGEDQSIYLQVTRGVAKRDH
                     AFPKDAAPTVFMMSNPLVTPSREQVENGATAVTATDFRWLRCDVKVTSLLANCLLRQR
                     AADAGADEVVMFRDGYLTEGSSSNVFAVKNGTLLAPPKNHLVLPGITYDVVLELAAMK
                     KVPVEVREIAEDEVRGADELWLTSSSKEVLAITRLDGRPVGAGRAGAVFKTVYQAFQE
                     FKRKVMRQAA"
     gene            complement(6872..8008)
                     /locus_tag="AC16CX_00205"
     CDS             complement(6872..8008)
                     /locus_tag="AC16CX_00205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR3010962.1"
                     /translation="MRPILLLLLSVFSSVLPAQQAAFPVPPALPVAASSYLLLDFNSG
                     QLLVAHNAQARVEPASLTKLMTAYLTFTALRQKRIRPEQVVPVSEFAWRVEGSRMFIE
                     PRKPVTIDELIRGMIVHSGNDACIALAEAIAGSEEAFAEMMNREARRLGLRNTHFVNA
                     TGLPHPQHYSTAQDLAELAAAVMRDFPEYYPLYSQKEYRYNNITQSNRNRLLWTDPTV
                     DGMKTGYTENAGYCLITSARRGERRLVSVVLGAASEAARAAESQKLLNYGFQAYDGLR
                     LYPGNQPVATLQVYKGGANSVKAGFLADLHLALPKGSGDKLKATVVSMQPLLAPISVG
                     QKIGTMKLTLDGTPFREIPVVALENVPVAGILGRGWDTLRLLFR"
     gene            8107..8589
                     /locus_tag="AC16CX_00210"
     CDS             8107..8589
                     /locus_tag="AC16CX_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HIR3010963.1"
                     /translation="MQDTRLAAAWFALAALVAVAGALPAGAAQGQPATVRGAWIRAPA
                     PGQKVAGAYMELVGRSPSALVSVTSPVAGRVELHSTAMKDGVMKMRPVGRIELAAGQT
                     VRLEPGGLHMMLIDLQRPLKPGEKVPLTLTIQRADFSRAVFTVQAEVREAAVAKAHQH
                     "
BASE COUNT         1528 a         2725 c         2870 g         1470 t
ORIGIN      
        1 cgaagggcgt gcacatcgtc tcggccgatt actcccagat cgagctgcgg atcatggcgc
       61 acatctcgca ggacgcaagc ctgctgaaag cgtttgcctc gggcgaggac atccaccgcg
      121 ccaccgccgc cgagatcttc ggcatggccc cggaggaagt gggcgcggag cagcgccgct
      181 acgccaaggt gatcaacttc gggctgatct acggcatgtc ggcgttcggg ctcgcccgcc
      241 agttggggct cgagcgctcc gccgcgcagc agtacatgga ccgctatttc gcgcgctacc
      301 ccggcgtcgc ggagtacatg aagcgcacgc gtgagacggc gcgtgagacg ggttacgtcg
      361 agacggtgtt cgggcggaga ctctggctgc ccgagatccg gggcggcaac ggcgcgcggc
      421 gccagggcgc ggagcgcgca gcgatcaacg cgccgatgca gggcacggcg gccgatctca
      481 tcaagatggc gatgatcgcg gtggaccgct ggcttgcgga gaaacgcctg agctcgagtc
      541 tcatcatgca ggtgcacgac gagctggtgc tggaagtgcc ggacaaggag ctcgatcggg
      601 tcaggcgcga attgccgaag ctcatgagcg gcgtgacgaa actctcagtg ccgctggtgg
      661 tggacatcgg cgccggcccc aattgggacc aggcgcatta gagcagtggg tcgtgattcg
      721 tgattggtga ttcgttcaag gagtcatcaa aggtgcggcc acccggctgc tctatttgag
      781 ggcgccgaag acttttctga cgatatcgct cgacgcgccg accgggttct tgcggaaggc
      841 cttttcctgc tcgccgatca tgaagtaaag gccgtcgagc gccttctgcg tgacgtgctg
      901 ctcgatggtc gcctggtcct ccttgacgag ccccagcgac gagccctggc cggcgatcgc
      961 gttgtacttc tgcgcgagcc cgacccgctc ggtcgccttc ttgacgatcg gcaggaagcg
     1021 ctgggtgagc tggggccggg tcgtgcgctt gaagtactcg gtgccggcgg tgtcgccgcc
     1081 ggtcaatatc ttcttggcgt cctgcacgga cattttcttg acggcgtcca ccagcagctg
     1141 ctttgcttcc ggcgccgccg attccgcggc gcggttcatg gcgagcacca gctcgtcggc
     1201 ctgctttctc atgccgagcg cgcgcatcgc gccttcgact cgcttcagcg acggcggaag
     1261 gggtattttc acctgtggat tgccgaaata gccgttctcg acgccgagct tggccaccgc
     1321 ggcaagcgag ccgtcgttga gcgcctgctt gaggccgctc accgcatcgg cgttgctgat
     1381 gcggtcaaga cccgccgccg gggcgtgccc gctgatgaca agtaacgcga agaaacccag
     1441 aatatggcgc atgatccact ccccgatgtc cgttctcttc ccgcggtccg agatgagcaa
     1501 gccccgcggc cctattctag ccgcggtcct ggcggcgggg gtaatgctgt tgctgatcgc
     1561 gattttcgcg gggacgactc ccgcgccgca ggtgaccttc gtctcgctca agggcgagaa
     1621 agtcacgacc gcggacctga aaggcagagt ggtgctggtc aacttctggg cgaccgactg
     1681 cccgatctgc atcaaggaga tgccggacat ggtgcggacc tacgagcgct accgcgatag
     1741 cggcttcgag ttcatcgcgg tagcgatgaa gcacgacccg cccaactacg tcattcatta
     1801 caccgagaac aaccggctgc cgttcaaggt cgcccttgat ccgatgggcg atctcgcccg
     1861 ggccttcggc gacgtgaagc tcacgccgac ctcgtttgtc atagacaagc gcggaaacat
     1921 cgtgctgcgc gtgaccggcg agccggattt cgccaggttg cacgagctgc ttgagaagaa
     1981 actgggtgaa gcggcttagc gttcccggtt taggcggtcc tctgccgcga ctgccacagc
     2041 acccagctca tgtaggcgac gatcgcgatg ttgatggcga agatcgtcac ctttgcccag
     2101 gagaggccgt gggcgatctc aacgatctcg atcggcagat agaccgcacc cgcgagcgcc
     2161 gcgaaccatt ctgcccaacg gcgttccttc cagagcccgt aagcctcgac gaaacgtgct
     2221 gcggcgtacc cggcggcagc ggaggcaagc agcgtgatct ccgcatcgtt gagcctgccc
     2281 gcggcgtcga gaaagatgct ggggtaatga ctggcaggat tcaggtgaaa ggtgcggacc
     2341 agttcctcgg cgtaggcttg gacgtcgcgg tggatcagcg acagcgcgcc gagacctgcc
     2401 gcgagcacca gcacgccttt tgcgccctcc aacagggcga ctgtcctggt gccacgcgta
     2461 tagcgcgtca tgcgcgatca cgcccgccag gggctacagc tcgggtggag aaaggctcag
     2521 acgatgcctg ccccgtgggc ctgccgatcg gcgtggtagc tggagcggac cagcggtccg
     2581 cacgcggcgt gcatgaagcc gagcgcatgt gcctgccgcg cgaattcatc gaacaccgcc
     2641 ggctcggcat agcgcgcgac cggcagatgg tgggcggatg gccggaggta ctggccgatg
     2701 gtgagcatat ccacgtcgtg ggcgcgcagg tcgcgcatca ccgaaaggat ttcctcatcg
     2761 gtttcaccca gacccaccat cagtcccgac ttggtgggta tccgtggaaa ccgcgatttg
     2821 aaatccttca atagcttcag cgagtgccaa tagtcggcgc cgggccgcgc ctgtcggtag
     2881 aggcgcggaa ccgtctcgag attgtggttc atgacgtcgg gaggcgccgc cgacagcacc
     2941 tcgagcgcga tctccagccg gccgcggaaa tccggcacca gcacttctat cctggtccgg
     3001 ggcgagagtt cgcgcacggc gcgtatgcag tcggcgaaat gcttcgcgcc gccatccccg
     3061 agatcatcgc ggtccacgct ggtgaccacc acgtagccga gcttgagtgc cgcgatggtg
     3121 cgcgcgaggt tgaacggctc ctcggggtcg ggcggcaatg gtcttccatg cgcgacatcg
     3181 cagaacgggc agcggcgggt gcacaggtcg cccaggatca tgaaggtcgc cgtacccttg
     3241 ccgaagcatt caccgatgtt cgggcaggtc gcctcctcgc acacggtgtg aaggcggtgt
     3301 tcacgcagga tgcgcttgat ttcctggaag cgctcgccgt tgccgagacg cacgcgaatc
     3361 cactccggct tggcgagcgg cgccgccggc accaccttga ccgggatgcg cgccgttttc
     3421 agccgtccct tctgcttttc accgagcgcg ctcatggcag cctatccagc aaatgccgga
     3481 cgagcttccc gcccaggatt tccctgtcgt cgcggatacc gagatcgcgc gcctgcgtca
     3541 cggccagtcc ggaatagccg cagggatcga tcacgtggaa gggggagaga tccatgtcga
     3601 cgttgaaagc gagcccgtgg tagcagcaac cgtttctcac gcgcaggccg agcgccgcga
     3661 tcttggcgcc gtcgacgtaa acgcccggcg cttcggtgcg tcctgcggcg gcaatgccgt
     3721 attcggcaag caagtctatc acggcgagct ccatcaaccg caccagagac cgcaccgcga
     3781 gcccccggcg ccgcaggtcg aggaggaggt agatgacgat ctggccgggg ccatggtagg
     3841 tgatctggcc gccgcgatcg gtcttgatca cggggatgcc gttcgccgcg cgcggcaggt
     3901 gctccgcgcg cccggcgact cccagcgtat acaccggcgg atgctcgagc agccacaact
     3961 cgtccgcgga attcgtcgaa cgcgttgagg tgaacgcctg catcgcgcgc caggtcggct
     4021 cgtaatcgac gcgcccgagc ctgtgcacgc gcacttcgga ggcttgccgg ctcccgtcga
     4081 cggaggcgtc agttgcaaca acagtcatgg tcatagatga taagaacggc ggcaaaatgc
     4141 cgcaaccctt gcgcggacac actcctgacc ggtcaagagc ggcgatgaaa aaatcattcc
     4201 tgattctggc tgttgcgacg gtggcgctcg catacggcgc cgcgctcgcc gccgaagcct
     4261 cgggcgacta cgccaccgat ctcgccaccg tgtacggcgg ctatcaacgg atgcttgcga
     4321 tgaaggaggc gtgcgacacg gcgatgcccg ccgcgcgtgc agccaacgag aaagcgttcg
     4381 tggcgtggcg cacgcagcat cgcgcgctga tccaggactt gcagcgccgc gtcacggcga
     4441 tgatccgcct tgcctcgcag gacgagaaag aatatgcgcg caacctcggg aagtacgaag
     4501 gggcgatcct gcaggagcgc gaggagtaca aggaaatcct gctcggcctg gggcaggacg
     4561 agttgcgcga gcagtgccag cgcatgccgg aaatgctcaa ggggccgggg gcggacctcg
     4621 gtaaagtcta cgcggccgag ctggagacca tccgcaaacg caaataagcg cgcatcggcg
     4681 cgggcgcggc ctcggaacgg aactcgcgga atcaagatgg tttgcatcgg ccgcctgcgc
     4741 gcctccgggg tcgcacccgc aagcgggcac ctgctccgcc ctgcggcgcg ctttgcgtat
     4801 acaatttctc cactggctga ttgatacggg agcgaaacca tgggaaaagg cgacaagcgc
     4861 acgcgccgcg gcaagctgtt ccgcggcagc tacggcaaga cgcggccggg aagaaagaag
     4921 aagaaaaaga agccgatggc ggcgagatag gcgtgcgacg gaaggacggt catggcgcag
     4981 ccgcaaccga acaagctcga ttcaggcgat gtgtttccga agctcgacct aatgcacacg
     5041 ctcgcgcacg gtgcactcgc actgcccggc gcgctggcgg gcaagtgggg ggtcatcctc
     5101 ttttatcgcg gccactggtg accctactgc cgtcagcagt tggctgactt ccagtctcgt
     5161 ctcgccgagc ttgagaagct cggtgcagca gttatcgcgg cctcggttga caacgaggcc
     5221 gacacccggc agttgatcga gaagctccag gtcacgttcc cggtggcgta cggcatcgac
     5281 ggcaggcgcg tgtgcgaggc aaccggggcg tactgggaag agaggcgcca gatcgtgcag
     5341 gcgaccggct tcctgctgcg cccggacggg cgcatagtca ctgcggtcta cagcagcgcg
     5401 caaatcggcc gtttcgtcgc ctccgatgtg ctccgcgtaa tcgacttcca gcagaaaaac
     5461 cagtgagcgg tgaacggtga gccgtaagcg gaaacgccca agaacctgct tgaacccgct
     5521 taccgcttac agcttactgc tcacctgtct ataagaccat caccaccatc gggtgatcgc
     5581 acaattcctg atagaggccg tcgagctgct cgcgcgaggt cgcgcggatc acgcaggtga
     5641 cgctcaagta cttgcggcgc ttgctcgccc tcaattccat ggtcgcgccg tcgaagtccg
     5701 gggcgtggcg cttgaccacc tcaagcacgg cctgcgcgaa gcccgcctgc gtgtgtccga
     5761 ggatcttgat cgggaagtcg caggggtatt cgagcagtgg ggtgtcggcg atcatgcggc
     5821 cacctcagac acggcatttc aattgcggag acagcgtgtc ggtggcccag cgtccttcgc
     5881 gggtcgcatc cccgacagcg gggcccctgc tccgccgctc atgccgcttg cctcatcacc
     5941 ttgcgtttga attcctggaa agcttggtag accgtcttga acaccgcgcc ggcccggccg
     6001 gcgccgaccg gcctgccgtc gagccgggtg atcgcgagga cttccttcga ggaagaggtc
     6061 agccacagct cgtcggcgcc gcgcacctcg tcctcggcga tctcgcgcac ttccaccggg
     6121 accttcttca tcgcggcgag ctccagcacc acgtcgtaag tgatgcccgg cagcacgaga
     6181 tggttcttcg gtggcgcgag gagcgtgccg ttcttcaccg cgaacacgtt gctcgacgag
     6241 ccctcggtga ggtagccgtc gcggaacatc accacttcat cggcgcccgc gtccgccgcg
     6301 cgctggcgca ggaggcagtt cgcgaggagc gaggtgacct tgacgtcgca gcgcagccag
     6361 cggaaatcgg tggcggtgac ggcggtggcc ccgttctcca cctgctcgcg ggacggcgtc
     6421 accagcggat tggacatcat gaacaccgtc ggcgccgcat ccttgggaaa ggcgtgatcg
     6481 cgcttcgcca cgccacgcgt cacctgcagg tagatcgatt ggtcctcgcc ggcgtggcgc
     6541 gcgacgaggt catgaaccag ctgcgtccat tcggcgtcgg tcatgggatt cgcgagccag
     6601 atcgcgtcga ggctgcgctg cagccgcttg aggtgctccg gcaggcggaa cgggcggcgc
     6661 gaatagaccg ggatgacctc gtagataccg tcgccgaaga tgaagccgcg atcaagcact
     6721 gaaacgcgtg cttgatcaag cggcaggaat tctccattga gataggcgat atcgctcatg
     6781 tggggctcac aggtgatgaa atggcgctgg cccgcgtcat ttcgattgcg gtcagtctac
     6841 acctcataaa atgccgcttg cgagcggcat tttatcggaa cagcagccgc agcgtatccc
     6901 agccgcgccc gaggataccc gccaccggca cgttttccag cgccaccacc gggatctccc
     6961 ggaacggcgt cccgtcgagc gtgagcttca tcgtgccgat tttctggccg acgctgatcg
     7021 gcgcgagcag cggctgcatg ctcacgacgg tcgccttcaa cttgtcgccg ctgcctttgg
     7081 ggagggcgag gtgaaggtcg gcgaggaatc cggctttcac gctgttagcg ccgcccttgt
     7141 aaacctggag cgtcgcaacc ggctggttgc cggggtacag gcgcaatcca tcgtaggcct
     7201 ggaagccgta attgagcagt ttctggcttt ccgccgcgcg cgccgcttcg ctcgccgcgc
     7261 cgagcaccac cgagacgagc cgccgctcgc cgcgccgcgc ggaggtgatc aggcagtagc
     7321 cggcattctc ggtgtagccg gttttcatgc cgtccacggt cggatcggtc cacagcaggc
     7381 ggttgcggtt ggattgcgtg atgttgttgt aacggtattc cttctgcgag tagaggggat
     7441 agtactccgg gaaatcgcgc atcacggccg ccgcgagctc ggcgaggtcc tgcgcggtgg
     7501 aataatgctg cgggtgcggg agccccgttg cattgacgaa atgggtgttc ctgagcccga
     7561 gccgccgcgc ttcgcggttc atcatctcgg cgaacgcctc ctcgctgccg gcgatcgcct
     7621 cggcgagcgc gatgcatgca tcgttgccgg agtgcacgat catgccgcgg atcagctcgt
     7681 cgatggtcac cggcttgcgc ggctcgatga acatgcgcga gccctcgacg cgccacgcga
     7741 actcggaaac gggaaccacc tgctcggggc ggatccgctt ctgcctgagg gcggtgaacg
     7801 tgaggtaggc cgtcatcaac ttggtgagcg aggccggctc cacccgtgct tgcgcgttgt
     7861 gggcgaccaa taactggccg ctgttgaagt ccagcagcag gtaggagctt gccgcgacgg
     7921 gaagcgcggg cgggaccgga aacgcggctt gctgggcggg aagaacggag gaaaagaccg
     7981 acaggaggag cagcaggatc ggacgcatgg cgagcgccga ttatagcaaa gcacccgcgc
     8041 gtccttgcgc gcttgcggga tcgcggcacg ggtgaaacaa tccgcccttt tcgggaggcg
     8101 aggggaatgc aagacacacg actcgccgcg gcgtggtttg ccctcgccgc cctcgtcgcg
     8161 gtggccggcg ccctgccggc cggagccgct caggggcagc ccgcgaccgt gaggggtgcc
     8221 tggatacgcg cccccgcgcc gggacagaag gtcgccggcg cctacatgga gcttgtcggc
     8281 cgctccccat ccgcgctggt ttccgtcacg agcccggtcg ccgggcgcgt cgagctgcac
     8341 agcacggcga tgaaagacgg cgtgatgaag atgcgccccg tcgggagaat cgagctcgcc
     8401 gcgggccaga cggtcaggct cgaacccggc ggcttgcaca tgatgctgat cgacctccag
     8461 cgccccctca agccgggcga gaaagtgccg ctcacgctca ccatccagcg cgccgatttc
     8521 agccgtgccg tgttcacggt gcaggccgaa gtgcgcgagg ccgccgttgc gaaggcgcat
     8581 caacactgat cac
//
LOCUS       DBNGWZ010000010         2402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3401244, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000010 DBNGWZ010000000
VERSION     DBNGWZ010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3401244"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="AC16CX_00215"
     CDS             <1..575
                     /locus_tag="AC16CX_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3010964.1"
                     /translation="LPLGAALAVWRFHGRQPVIILLNALMGLPPVVVGLLVYLLLSRA
                     GPLGSFGILFTPAAMVVAQTILILPIVAALSRQVIEDAWQEYREQLRSLGAGPLASAL
                     TLLWDTRFSLITVVLAGFGRAAAEVGAVIIVGGNIDGVTRVMTTAIALETSKGDLPLA
                     LGLGIILLALVVAVNAAAYWVKEAAQRRYG"
     gene            584..1282
                     /locus_tag="AC16CX_00220"
     CDS             584..1282
                     /locus_tag="AC16CX_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3010965.1"
                     /translation="MLPLVLDDVSFTVNGRTLIDRVSVEIGAGPRTIILGPNGAGKSV
                     LMRLCHGLLRPTSGSILWKGPNAGRQRQAMVFQRPVVLRRSALANVEYGLKLAGTAAR
                     ERGLRARDVLEAVGLADVAERPARVLSGGEQQRLALARAWALGPEVLFLDEPTANLDP
                     GAAREVENIVGQIRASGTKIIMTTHNLGQARRLGDEILFLHQGRLVERAPVEHFFSRP
                     GSPEAASFIRGEVP"
     gene            1285..2115
                     /locus_tag="AC16CX_00225"
     CDS             1285..2115
                     /locus_tag="AC16CX_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3010966.1"
                     /translation="MAEKLLSAATFVLMVFWAVTVSADGCYITIASTTSTEQSGLFRH
                     ILPIFEKKTGIQARVVALGTGQALDMARRGDADVVFVHARSAEERFVAEGYGVKRLPV
                     MYNDFVLIGPKSDPAKIVGSKDVLDALRKIRAATAPFVSRGDRSGTHIAELALWKQAG
                     IDIATGKGPWYRDTGQGMGPALNTAASMNGYILADRGTWISFKNRGDLAILVEGDKRL
                     FNQYGVILVNPEKHKHVKKDMGQAFIDWIVSPEGQKAIAGYNIGGEQLFFPNAGQEGA
                     "
     gene            2156..>2402
                     /locus_tag="AC16CX_00230"
     CDS             2156..>2402
                     /locus_tag="AC16CX_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HIR3010967.1"
                     /translation="MNPSVPPGVPGAAAQPVGRAALRFDRNEWSGAFGDLGTLVPFIL
                     AYITIVGVEPAGMLLAFGIAYIAAGAVFRTPFPVQPMK"
BASE COUNT          393 a          740 c          838 g          431 t
ORIGIN      
        1 ggctgcccct gggggcggcg cttgccgtct ggcgcttcca cggccgccag ccggtcatca
       61 ttctcctcaa cgccctgatg ggactgccgc cggtcgtggt cggcctgctg gtctatctcc
      121 tgctgtcgcg ggcgggaccc ctgggctcgt tcggcatcct gttcacgccc gccgccatgg
      181 tagtggcgca gacgatcctg atcctgccca tcgtggcggc gctgtcgcgg caggtgatcg
      241 aggatgcgtg gcaggaatat cgggagcaac tgcgttccct gggggccggt ccgctggcct
      301 ccgcgctgac cttactttgg gacacgcgct tctcattgat caccgtggtt ctggcgggat
      361 ttggacgtgc tgcggcggag gttggggcgg tgatcatcgt gggcggcaac attgacggcg
      421 tcacgcgcgt catgaccacg gcgattgcgc tggaaaccag caagggcgac ctcccgctcg
      481 cgctgggact gggaatcatc ctgctggcgc tggtggtcgc cgtgaacgcc gcggcgtact
      541 gggtcaagga agccgcgcag cgacgctacg gctgagtctc gccatgttac cgctggtact
      601 cgatgacgtt tccttcacgg tcaatggccg gactctcatc gaccgcgttt cggtcgagat
      661 cggcgccggg ccgcgcacga tcatcctcgg gccgaacggg gccgggaaaa gcgtgctgat
      721 gcggctttgc cacggtctgc tgcgcccgac ctcaggcagc atcctctgga agggcccgaa
      781 cgccggaagg cagcgccagg ccatggtgtt ccagcgccca gtggtactgc gccgttcagc
      841 gctggccaac gtggagtacg gtctgaaact ggcggggacc gcagcgcgcg agagaggact
      901 gcgggctcgc gacgtcctcg aagcggtggg attggcggat gtcgccgagc gtccggcgcg
      961 ggtgctctcg ggcggcgagc aacaacggct cgcgctcgcc cgggcgtggg cccttggccc
     1021 cgaagtgttg ttcctcgacg agccgacggc caatctcgat cccggcgccg cgcgcgaggt
     1081 ggagaacatc gtcggccaga tccgggcgag cggcaccaag atcatcatga ccacgcacaa
     1141 cctggggcag gcccggcggc tgggggacga gatcctgttc ctgcaccagg gccggctggt
     1201 cgagcgcgcg ccggtggagc atttcttctc ccggccgggc tcgcccgagg ccgcttcgtt
     1261 tatcagaggg gaggtcccgt agccatggcg gagaaactgc tgtctgcggc cacattcgtg
     1321 ctgatggttt tttgggcggt cactgtatca gcagacggtt gttacatcac gatagcttcc
     1381 acgacttcga ccgagcagtc ggggctgttc aggcacatcc tgccgatttt cgagaagaag
     1441 accggcatcc aggcgcgggt ggtcgcgctt ggaaccgggc aggcactgga catggcgcgg
     1501 cggggggatg ccgacgtggt gttcgtgcac gcaagatccg cagaggagcg gttcgtcgcc
     1561 gagggctacg gtgtcaagcg cctgccggtg atgtacaacg atttcgtgct gatcggaccg
     1621 aagtcggatc ccgcgaagat cgtgggtagc aaggacgtcc tcgacgcgct gcgcaagatc
     1681 agggccgcga ccgcaccgtt cgtgtcgcgg ggagaccgga gcggcacgca catcgcggaa
     1741 ttggcattgt ggaaacaagc gggcatagat atcgcgaccg gtaaaggtcc gtggtatcgc
     1801 gatacgggac agggcatggg cccggcgctc aacacggccg cttcgatgaa cggctacatc
     1861 ctcgccgatc gcggcacctg gatctcgttc aagaaccgcg gcgatctcgc gatcctggtc
     1921 gaaggcgaca agcggctgtt caaccagtac ggcgtgatac tggtgaaccc ggagaagcac
     1981 aagcacgtga agaaggacat gggtcaggca ttcatcgact ggatcgtctc ccccgaagga
     2041 cagaaagcca tcgccggtta caacatcggc ggcgagcagc tgttcttccc caacgccggc
     2101 caggagggcg cgtgatggtt gcgcgcgact gaggggcgat atacgctgct tccgcatgaa
     2161 tcccagcgtg ccgcccggcg tcccgggagc cgcagcgcaa cctgtcggtc gagcagcgct
     2221 tcggttcgac cgcaacgaat ggtcgggagc gttcggcgac cttggcacgc tggtgccgtt
     2281 catactcgcc tatatcacca tcgttggcgt cgagcccgcc ggcatgctgc tggcgtttgg
     2341 aatcgcgtat attgccgccg gtgcggtttt ccgcacgccg ttcccggtgc agccgatgaa
     2401 gg
//
LOCUS       DBNGWZ010000011         4432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_504574, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000011 DBNGWZ010000000
VERSION     DBNGWZ010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_504574"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="AC16CX_00235"
     CDS             <1..890
                     /locus_tag="AC16CX_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR3010968.1"
                     /translation="LGRGVDIDSFGKGIFFLMKSNHTDLFEEAAKFRAMRRIWATRLR
                     DRYGAKDAETMKCRILAHSGGSLLTRERPELNIARTTLACVAGVLGGIQLIGLRTMDE
                     VFGIPTEKSELIAIGTQHVVAYETGLPDVVDPLGGSYYVEALTSEFERRVLDELERIN
                     RMGGMVKAIESGYVRKAIAQDSYRTQVAWETGRKVRVGVNKFRIEENEPPRTPYAYKP
                     EEEERQVEAVKRLRRERDNAAVGKALERVIELARRPAASENNLMPALIEAVRAYATIG
                     EICGALKQVFGEYVEPTRF"
     gene            900..1316
                     /locus_tag="AC16CX_00240"
     CDS             900..1316
                     /locus_tag="AC16CX_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR3010969.1"
                     /translation="MGGQQRRIRVLMGKPGLCGHDRGIVLVVMALREAGMEVIYSGRH
                     NSPESLVEAAIQEDVDVLGVSILAGGHLGLCERIAALLRERKAEDIVLLAGGFIPPED
                     IPKLKAFGVREVFRDGAKLEEIVQYIEKVGRERATA"
     gene            1353..2693
                     /locus_tag="AC16CX_00245"
     CDS             1353..2693
                     /locus_tag="AC16CX_00245"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3010970.1"
                     /translation="MGVTETLTRFLLQTTYEDFPRETVDRAKHLAIDFVGGALAGCQT
                     HVSNIFTDYVKQMAAKPEAVIIGKNFRTTAQYASYLNGIFNHSTELEAVSQRTSPNPL
                     AVIATSLSLGDKMKLPGKKVLEGLILGFEIQGRVGGPSLKGVSKKGRISIFNHLGTAS
                     AASKLMGLNLEQSRMAIGTAAFQAGGLITTVGTMAHVTELAFGSRDGIEAAELARKGF
                     TSHPDIIETPQGFCDALIEEGAYDLNEMTRDLGKTFQIVNPGISVKKYPCCYRSHRAL
                     DAFYDMVDEHHISHGDIDKVIVEMNLYDSYLMKFPEPKTGEEAKFSFRHIFGTALLKG
                     KVWIDSFSDEAASDRRYVEARRRVEEVVHRDWPPGRVDARIPVTIKLKDGRTFSKENA
                     SPREPTSNQLLDRYREGAEGVLAPEQAKKSMELLLDMENSRDFSEIMGLLSAKS"
     gene            2791..4185
                     /locus_tag="AC16CX_00250"
     CDS             2791..4185
                     /locus_tag="AC16CX_00250"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3010971.1"
                     /translation="MPQTIAERLAQFSYDLSYDDIPPEVVDKAKRLILDTLGVSLGST
                     RIDFGKSALDLVAGWGGAPEATLIGRRIRVPAHNAALCNGILGHGQDYDDTHTESVVH
                     PSAALVPVALAVAERTGRSGREMLAALVGGMEATIRIAIPALNRFHLRGFHTTSVAAT
                     FGAALIAARLEKLGLRRTVDAVGIGGSFTSGLLECIPAAAGAKRLHAGWGALCGIIAA
                     QLARGDYTGPNTVFEGKLGVYNSFLRGESLDLSVIFNGLGRDWETLDVRPKLYPCCHY
                     LQSFLDCAAALRREHEMRAEDIAGVSCRIAQGAVNIVCEPWEKKLAPVTGYDARFSLP
                     FAVALMLARGRAGVAEFSAQSAADPQIRQLMAKVSYAVEPKYEVKDMPGWIEVTLKDG
                     TRRRWEIPEVRGNARNPVALEELLDKFNANTSFLGAAASSRIADDVLALDRTADLRGF
                     MARLCEREAAGSVA"
     gene            4200..>4432
                     /locus_tag="AC16CX_00255"
     CDS             4200..>4432
                     /locus_tag="AC16CX_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3010972.1"
                     /translation="MIGDRPRPQPEESVAGILRGDRGAIARAISAVENETEAGRAIMR
                     AIHPHLGRAQVLGVTGAPGAGKSTLVSELIGEFT"
BASE COUNT          862 a         1295 c         1509 g          766 t
ORIGIN      
        1 cgctcggccg cggcgtcgac atcgacagct tcggcaaggg catcttcttc ctcatgaagt
       61 ccaaccacac cgacctgttc gaggaggccg ccaagttccg cgccatgcgc aggatctggg
      121 cgacgcgctt gcgcgaccgc tacggcgcga aggacgcgga aacgatgaaa tgccgcattc
      181 tggcgcacag cgggggctcc ctgctcacgc gtgaacgtcc cgagctcaac atcgcccgca
      241 ccacgctcgc gtgcgtggcc ggcgtactgg gcggaatcca gttgatcggg ttgcgcacca
      301 tggacgaggt gttcggcatt ccgaccgaga aatccgagct gatcgccatc ggcacgcaac
      361 acgtcgtcgc ctacgagacc ggcctgccgg acgtggtgga tccgctcggg ggatcgtatt
      421 acgtagaagc gctgacctcc gaattcgagc gccgggtcct tgacgagctg gagcgcatca
      481 atcgtatggg cgggatggtg aaggcgatcg agtcgggtta cgtccgcaag gccatcgccc
      541 aggattcgta tcgcacgcag gtcgcgtggg agacgggccg gaaggtgagg gtcggcgtca
      601 acaagttccg catcgaggag aacgagccgc cgcgcacgcc ctatgcatac aagccggaag
      661 aggaggagcg ccaggtcgag gcggtcaagc ggttgcgccg cgagcgggac aacgccgcgg
      721 tcggcaaggc gctcgagcgc gtgatcgagc tggcgcggcg gccggcggcg agcgagaaca
      781 acctgatgcc ggcgctgatc gaggcggtgc gcgcatatgc gacgatcggg gagatctgcg
      841 gcgcgctcaa gcaggtgttc ggcgagtacg tcgagccgac ccgcttctga gggggcacga
      901 tgggcggcca gcagcgaaga atccgcgtcc tgatgggcaa gccgggcctg tgcggccacg
      961 accgcggcat cgtgctggtc gtgatggcgc tgcgggaggc cggcatggag gtgatctact
     1021 cgggccggca caactcgccg gagagcctgg tcgaggccgc catccaggag gacgtcgacg
     1081 tgctcggggt gagcatactc gccggcgggc atctcggcct gtgcgagcgc atcgccgcgc
     1141 tgctgcgcga gcgcaaggcg gaggacatcg tcctgctggc gggggggttc attccgcccg
     1201 aggacattcc gaagctcaag gcgttcggcg tgcgcgaagt cttccgggac ggcgcgaagc
     1261 tggaagagat cgtccagtac atcgaaaagg tcggcaggga acgcgccacg gcataggccc
     1321 acgatgcgca gtcggatgaa aggagtgtaa ggatgggggt aaccgagaca ctaacgaggt
     1381 tccttttaca aacgacatac gaagatttcc cgcgggagac cgttgatagg gccaagcatc
     1441 tggccatcga ttttgtagga ggagctttgg caggttgtca gacccacgtg agcaacatct
     1501 tcaccgatta cgtgaagcag atggctgcca aacccgaagc agtgatcatc ggaaagaatt
     1561 tcagaacgac cgcccagtat gcctcttatc tgaacgggat ctttaaccat tccaccgaac
     1621 tcgaggccgt gagccagcgc acctctccga acccgctggc cgtgattgcc acgtctcttt
     1681 ccctcggaga caagatgaag cttccgggga aaaaggtgct ggaggggttg atcctcgggt
     1741 ttgagatcca gggcagagtg ggtggtccat ccttgaaggg ggtcagcaag aaaggaagga
     1801 tcagtatttt caatcacctt gggacggctt ccgccgccag taaattgatg ggattgaatc
     1861 tggaacaatc gagaatggcg attggcaccg ctgcttttca agcggggggc ttgattacca
     1921 ccgtgggaac gatggcccat gtcacggaac tggcctttgg gtcgcgcgat ggcatagagg
     1981 cggcggaact ggcaaggaag ggattcacct cccatcccga catcatcgag actccccagg
     2041 ggttttgcga tgcgttgatt gaggaggggg catacgacct gaatgagatg acccgggacc
     2101 tggggaaaac ctttcagatc gtgaatccgg gcatttccgt caagaagtat ccgtgctgtt
     2161 atcgttccca tcgcgccctg gatgcgtttt acgatatggt ggacgagcac catatttctc
     2221 atggtgacat cgacaaagtc atcgtggaga tgaacctgta cgattcctat ctgatgaaat
     2281 tcccggagcc aaaaaccgga gaggaggcga aattcagctt tcgccatatt ttcgggacgg
     2341 ccctcttgaa agggaaagta tggatcgatt ctttttccga tgaggccgct tcggatcgca
     2401 gatacgtgga agcccgcaga cgggtggaag aggtcgtcca tcgcgattgg cccccgggcc
     2461 gggtggacgc ccgcattccc gtcacgatca agctgaaaga tggaaggacc ttctcaaaag
     2521 agaatgcgtc tccccgcgaa cccacgtcga atcaattgtt ggataggtat cgtgagggtg
     2581 ctgaaggggt gctggcccct gagcaagcga aaaaatcgat ggagctgttg ctggatatgg
     2641 aaaactcgcg ggatttctcg gagatcatgg gtcttctgag tgccaaatcc tgatgccttc
     2701 gccttcttct gcaaaacgcg ctacacctgc ctgagctgtc accggaagcg cacgccgatc
     2761 gcgcgcgccc tggccgaata aggagcggat atgccacaga ccatcgcgga gcgcctcgcg
     2821 caattcagct acgacctgtc ctacgacgac attccgcccg aggtcgttga caaggcgaag
     2881 cggctgattc tcgacacgct cggcgtcagc ctgggttcga cccgcatcga tttcgggaaa
     2941 tccgcgctcg acctcgtggc cggatggggc ggcgcgccgg aagcgacgct gatcggacgc
     3001 cgcatccggg tgcccgccca caacgccgcg ctgtgcaacg ggatactcgg tcacgggcag
     3061 gactacgacg atacgcacac cgagtcggtg gtccacccca gcgccgccct ggtgccggtt
     3121 gcgctcgcgg tggccgagcg cacgggccgc tccggccgcg agatgctggc ggcgctggtc
     3181 ggcggcatgg aggcgacgat ccggatcgcg atcccggcct tgaacaggtt ccatttgcgg
     3241 ggatttcaca cgacctcggt cgccgcgaca ttcggggccg cgctgatcgc cgccaggctc
     3301 gaaaagctgg ggctgcggcg cacggtcgac gctgtcggca tcggcgggag cttcacgtcg
     3361 ggactgctcg aatgcatccc ggcggcggcg ggcgcgaagc gtctgcacgc cggctggggc
     3421 gcgctgtgcg gaatcatcgc ggcgcagctc gcgcgcggtg actacaccgg cccgaacacg
     3481 gtcttcgagg gcaagctcgg ggtctacaac tcgtttctgc gcggcgagtc gctcgatttg
     3541 agcgtgatct tcaacgggct cggccgcgac tgggaaacgc tcgacgtccg tcccaagctc
     3601 tacccctgct gccattatct gcagtctttt ctcgactgcg cggcggccct gcggcgggag
     3661 cacgagatgc gcgccgagga catagccggc gtttcctgcc ggatcgcgca gggcgcggtc
     3721 aacatcgtgt gcgagccgtg ggagaagaaa ctcgcccccg tgaccggata cgacgcgcgc
     3781 ttcagcctcc ccttcgcggt ggcgctgatg ctcgccaggg gcagagcggg cgtggcggaa
     3841 ttttcggcgc aaagcgccgc ggacccgcag atccggcagc tgatggcaaa agtgagttac
     3901 gcggtggaac cgaaatacga ggtcaaggac atgccgggct ggatcgaggt gacgctcaag
     3961 gacggcaccc ggcgcaggtg ggagatcccg gaggtgcgcg gcaatgcgag aaaccccgtg
     4021 gcgctggagg aattgctgga caagttcaat gccaacacct cctttctcgg ggcggctgca
     4081 agctcgcgca tagcggacga tgttctcgcc ctggacagga cggccgacct gcgcgggttc
     4141 atggctcgcc tctgtgagcg ggaagctgcc gggagcgtgg cgtgagtcac gctggaccag
     4201 tgatcggtga tcgacccagg ccgcagccgg aggagtcggt agcgggcatc ttgcgcggcg
     4261 accgcggggc gattgcgcgc gcgatttcgg cggtcgagaa cgagaccgag gcgggtcgcg
     4321 cgatcatgcg ggcaatccac cctcatctcg gccgcgcaca ggttctgggt gtcaccggcg
     4381 ctcccggcgc cggcaaatcg acgctggtga gcgaactgat cggggagttc ac
//
LOCUS       DBNGWZ010000012         4218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_882511, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000012 DBNGWZ010000000
VERSION     DBNGWZ010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_882511"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16CX_00260"
     CDS             complement(<1..413)
                     /locus_tag="AC16CX_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3010973.1"
                     /translation="MGTILTLHDPQTARAYYEAGYWRGETMYALASRHAAARPGAYAL
                     RDSTKRLTWSELVHWTDALAADLNRAGLRRGSRVSVWLPNRAEAVVALLACSRMGYVC
                     NPSLHQNYTVAEVVQLLERIQSAALFVHPGYGADA"
     gene            complement(538..1317)
                     /locus_tag="AC16CX_00265"
     CDS             complement(538..1317)
                     /locus_tag="AC16CX_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3010974.1"
                     /translation="MTYETILYEEDGAIGWLTLNRPDDGNMFTPVMCHEIRDCINGIR
                     RETRTRVLVITGAGDKFFCIGGRKEGMEDSTLYTGVLPTLEMYESIDRLQKPVIASVN
                     GYAVGGGQVLQVMCDLTIAKESAVFRQVGPMMGSFDAGYGTWYLEDLVGKKKAKELWY
                     LNPRLSAREALEIGLINKVVPDDKLREETRKMAQEVAERGAFALAAIKAAFTARHGGV
                     GGLGRLSHDLLLRLYLDTEESKELGVAFGEKRKPDPKKFGH"
     gene            1518..2282
                     /locus_tag="AC16CX_00270"
     CDS             1518..2282
                     /locus_tag="AC16CX_00270"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR3010975.1"
                     /translation="MAVNQGVVQKTSHRIGLLVPSSNTTVEPEFYRALPADVTLHTAR
                     LFLTEITPEAILRLVEELETQSRLLASADVDVIMLGATAPSFLKGAGYDREVTARIEK
                     ATGRPATTTSTALLRALRFLDAKRIVLGSAYDDKVNAIARGFLEANGFKVVAARGLGM
                     VDNLAVGRLGPESAYELARGADRADADAMVLACTNWRTMDTIERLERELGKPVVSTTQ
                     VSVWDALRMIGYRREVPGYGQLLRSLAAEGRLAAVA"
     gene            2302..3069
                     /locus_tag="AC16CX_00275"
     CDS             2302..3069
                     /locus_tag="AC16CX_00275"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR3010976.1"
                     /translation="MYGWRGRIGLLVPSINTTMETEFWRIAPAGVSVHSARIAGGRHG
                     TPEELRGMENASKQAAQEVAMVEPDVVVYGCTSGSFFEGPEWNRRIAEQLTEIAGAPT
                     VTTAGAMAACLLAGGHKRVDVVTPYVELTNERLRQFLKAHDIDVVTLGTFDMLDMFDH
                     AKIEPSEIYRKVREIATPRSEAVFIACTQLRALEVLDSLERDLGKPVYSAVQASAWEA
                     YQAMGVDPSITDCGSLLRKLSEPGAVKQPPRRLRKSA"
     gene            3136..>4218
                     /locus_tag="AC16CX_00280"
     CDS             3136..>4218
                     /locus_tag="AC16CX_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3010977.1"
                     /translation="MNARVETFDVVVVGGGGAGLAAAAEAARLGRRVAVLEKNPRLGG
                     STAWSVGSISITNSPHQIERGIRDTPQEHFEDLGLLAGAYAPRDNLPLRRILADNTND
                     VFEWLRGLGLEFAGPMIEPPHRYPRMHLVLPNSRAFPHVLGRHCAKLGVDIRLDVRAE
                     RLIVENGRTAGVQARGAGGSSAEFRARGGVVLAAGDYSAGRELKARFAGEDVAALEAV
                     NVTATGDGHRMALEIGAVVVNGDIVRGPIMRFVPPPHDRWILKLPPWRWLARSMRLAM
                     DRLPDAWLRPFAMSFLTTALGPSPELFKSGAILVNRSGERFADELAAPARAVPRQPGE
                     LAYIVFDRTLAQRFTAWPHFVSTAPGI"
BASE COUNT          686 a         1394 c         1433 g          705 t
ORIGIN      
        1 tcggcgtccg cgccgtatcc ggggtgcacg aacagcgcgg cgctctggat gcgctcgagc
       61 agctgcacca cctcggccac cgtgtagttc tggtgaagcg aggggttgca cacatagccc
      121 atgcgcgagc aggcaagcag cgccaccacc gcctcagccc ggttcggcag ccacactgag
      181 actcggctcc cgcgccgcag ccccgcccgg ttgaggtccg cggcaagcgc atccgtccag
      241 tgcacgagct cgctccaggt cagccgcttc gtgctgtcac gcagggcgta agcgccgggg
      301 cgcgccgccg catgccggct ggcgagcgca tacatcgtct cgccgcgcca gtatcccgct
      361 tcgtagtaag cgcgcgcggt ctgcggatcg tgcagggtca gtatcgtgcc cacaattttg
      421 aatgcttagt ttggattccc gattttgaat atcagagtgg gtctcaattt ctcgcgaaac
      481 tgccagagcc gcattcaaaa ttaatgaatt tcaaatcaag aaattcggac atggtcacta
      541 atgtccgaat ttcttagggt cgggcttgcg cttttcgccg aacgcaacgc ccagctcttt
      601 cgattcctcg gtgtcgaggt agagtctcag caacaggtcg tgcgacaggc gcccgagtcc
      661 gccgacgccg ccatggcgcg cggtgaaagc ggccttgatc gcggcgagcg cgaaagcgcc
      721 acgctcggcg acctcctgcg ccatcttgcg cgtctcctcg cgcaacttgt catccggcac
      781 gaccttgttg atgagtccga tctcgagcgc ctcgcgcgcg ctcaacctgg gattgaggta
      841 ccagagttcc ttcgccttct tcttgccgac gaggtcctcg aggtaccacg tgccgtagcc
      901 ggcgtcgaag ctgcccatca tcgggccgac ctgacggaac accgcgcttt ccttggcgat
      961 ggtgaggtcg cacatgacct gcagcacctg cccgccgccg accgcgtagc cgttgaccga
     1021 ggcgatgacc ggcttttgca gccggtcgat gctttcatac atctccaggg tgggcagcac
     1081 gccggtgtag agcgtactgt cctccatgcc ttccttacgc ccgccgatgc agaagaattt
     1141 gtcgcccgcg ccggtgatca ccagcacgcg agtacgcgtc tcgcgccgga tgccgttgat
     1201 gcagtcacgg atctcgtggc acatgaccgg cgtgaacata ttgccgtcgt ccggacggtt
     1261 caacgtcagc cagccaatcg cgccgtcttc ctcgtagagt atggtttcgt aggtcatcgg
     1321 aggggcggtg ccggccgtgc gtgggtcatc gcactccctg cgtcggccgg tatgtccaga
     1381 tagtgggctg gttgcgcgag cgcggcctcg gttgcgatgc cgttcgagtc cgtattctct
     1441 ttgacgggtg aaacgacgga ctcttatact accgcactca tcaggcgttg gggcggcggc
     1501 ccgatccagg aggacaggtg gcggtcaatc agggcgtggt gcagaaaacc tcgcatcgca
     1561 tcggactgct ggttccatcc tcgaacacca cggtcgagcc ggagttctat cgcgcgctgc
     1621 cggcggacgt cacgctgcat actgcgcgac tctttctcac cgagatcacg ccggaggcga
     1681 tcctcaggct ggtggaggag ctggagaccc agagccggct gctcgcgagc gcggacgtcg
     1741 atgtcatcat gctcggcgcc accgccccga gcttcctcaa gggggccggc tacgaccgcg
     1801 aagtcaccgc ccggatcgaa aaagcgaccg ggaggccggc gaccaccacg tccaccgcgc
     1861 tgctgcgcgc gctgcggttc ctcgatgcga agcgcatcgt gcttggctct gcgtacgacg
     1921 acaaggtcaa cgcgatcgcg cggggctttc tcgaggcgaa cgggttcaag gtggtcgccg
     1981 cccgcgggct cggcatggtg gacaacctcg cggtcggccg gctcggtccc gagagcgcgt
     2041 acgagctggc gcgcggcgcc gaccgggcgg atgccgacgc catggtgctc gcgtgcacca
     2101 actggcggac gatggacacg atcgaacggc tcgagcgcga gctcggcaag ccggtcgtct
     2161 ccaccaccca ggtcagcgtg tgggacgcgc tgcggatgat cggctatcgc agggaagtgc
     2221 cgggttacgg acagctgctg cgctcgctgg cggcggaagg ccgtctcgcg gcggtggcat
     2281 aggcagaaac aggaggcggt gatgtacgga tggcgaggca ggatcggttt gctggttccc
     2341 tcgatcaata ccacgatgga gacggagttc tggcgcatcg caccggcggg cgtctcggtg
     2401 cacagcgcgc gcatcgcggg cgggcgccac ggcacgcccg aagagctgcg cggcatggaa
     2461 aacgcgagca agcaggccgc gcaggaagtg gcgatggtcg agccggacgt ggtggtctac
     2521 ggctgcacca gcggcagctt cttcgaagga ccggagtgga accggcggat cgccgagcaa
     2581 ctgaccgaaa ttgcgggcgc gccgacggtc accaccgcag gggcgatggc ggcgtgtctg
     2641 ctcgccggcg gccacaagcg ggtggacgtg gtcacgccgt acgtggagct taccaacgaa
     2701 cggctgaggc agttcctgaa ggcgcacgac atcgacgtgg tgacgctcgg gaccttcgac
     2761 atgctcgaca tgttcgacca cgcgaagatc gagccctccg agatctaccg caaagtgcgg
     2821 gagatcgcga cgccgcgctc cgaggcggtg ttcatcgcgt gcacgcagct gcgtgcgctg
     2881 gaggtgctgg acagtctcga gcgcgacctc ggcaagcccg tctattcggc ggtccaggcg
     2941 agcgcgtggg aagcatacca ggcgatgggc gtcgacccta gcatcacgga ctgcggcagc
     3001 ctgttgcgca agctctccga accgggcgcc gtgaagcaac caccgcggcg gctgcgcaaa
     3061 tcggcctgag acgcagcgcc cgataagtcg aaggcaggcc tgcggcctgc cttcgttgtt
     3121 tctgaaaatg gaatcgtgaa cgctcgcgtt gaaacgttcg atgtcgtggt ggtcggcggc
     3181 ggcggtgccg gactcgccgc ggccgcggag gccgcgcggc tcggccgccg cgtcgccgtg
     3241 ctcgagaaaa accccagact cggcggcagc acggcgtggt cggtaggctc gatctcgatc
     3301 accaactcgc cgcaccagat cgagcgcggc atccgcgaca cgccgcagga gcatttcgaa
     3361 gacctggggc tgctcgccgg agcctacgcg ccgcgcgaca acctgccatt gcgccgcatc
     3421 ctcgccgaca acaccaacga tgtcttcgag tggctgcgcg gcctcggcct ggaattcgcc
     3481 gggccgatga tcgagccgcc gcaccgctat ccgcggatgc atctcgtcct gcccaactcg
     3541 cgcgcctttc cgcacgtcct cggccgccat tgcgcgaagc tcggcgtcga catccgcctc
     3601 gacgtgcgcg ccgagcggct gatcgtcgag aatggccgga cggccggcgt gcaggcgcgc
     3661 ggcgccggcg ggtcgagcgc cgaattccga gcgcgcggcg gcgtggtgct cgcggcgggc
     3721 gactacagcg ccggacgcga actcaaggcg cgctttgcgg gcgaggacgt cgccgcgctc
     3781 gaggccgtga acgtgaccgc gaccggcgat gggcaccgta tggcgctcga gatcggcgcg
     3841 gtcgtggtca acggcgacat cgtgcgcggg cccatcatgc gcttcgtgcc gccgccgcac
     3901 gaccgctgga tcctgaagct cccgccgtgg cgctggctgg cgcgctcgat gcggctcgcc
     3961 atggaccgtc tgccggacgc ctggctccgg ccgttcgcga tgagctttct caccaccgcg
     4021 ctggggccct ccccggagct gttcaagagc ggcgcgatcc tggtgaaccg cagcggcgag
     4081 cgcttcgccg acgagctggc ggcgccggcg cgcgcggtgc cgcgccagcc gggggagctc
     4141 gcctatatcg tttttgaccg gacgctcgcc caacgcttca ccgcgtggcc gcatttcgtg
     4201 tcgacggcgc cgggcatc
//
LOCUS       DBNGWZ010000013        11619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1638266, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000013 DBNGWZ010000000
VERSION     DBNGWZ010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1638266"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="AC16CX_00285"
     CDS             complement(<1..977)
                     /locus_tag="AC16CX_00285"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3010978.1"
                     /translation="MAVAPHALAAQSALDVLRESGNALEAMLAAAATITVAYPHMNSV
                     GGDSFWLVQVPRRPPRGIDACGAAARAASIEWYAARGITRAIPYRGGAAANTVAGAVS
                     GWELAFRLSRKWGGKLPLKRLLADAIHYAEHGIAVTRSQSSTTAAKRGELEPQPGFRE
                     TFLAGGGSPEGSLFKQPRLAATLARLARAGLEDFYRGKLARAIARDLAAAGSPLALDD
                     LGRHRAKFVAPLALEHSLGTVYNLPPPTQGLVSLLILGMLDALEVGSTGPASADYVHL
                     AVEATKRAFGVRDHCITDPAYMTVDPRALLAPGKIRELAADVDRGRAAR"
     gene            complement(1117..2403)
                     /locus_tag="AC16CX_00290"
     CDS             complement(1117..2403)
                     /locus_tag="AC16CX_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3010979.1"
                     /translation="MLELLALPFFACLVLTAIHVYLGFHVLARGVIFVDLTLAQIAAL
                     GLTVAILAGHPVHSEAAYWYALAFAIGGGILFALTRAQEEKVPQEAIIGIIYAVSAAL
                     AVLVVDRAPQGAEHIKQLLVGSILSVSANDVLELAVLYSAIGALHWAVRRPMLQLSLD
                     PAAAAGRHVLLWDMLFYATFALVVTSSVRVAGVLLVFSYLVVPAAIGALLARTVAARL
                     WIGWTVGGAVSAAGLAASWAWDLPTGAAVVSAFGAAIALVALGLGLRRASRAVRARGA
                     AALAPALVVLCGAVALGGALLVAFPRMDHLWLDALEHAAPRLQEWFLTPAEREARRDA
                     AESVIRAEAELARLRAVAQEVRWGTRTMDADQEERLRQYIAGRSEISAGDQLALRKLR
                     AKARERQRYWLGVPLLLVSLGLLLIMLRRAAPLRKK"
     gene            complement(2410..3336)
                     /locus_tag="AC16CX_00295"
     CDS             complement(2410..3336)
                     /locus_tag="AC16CX_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR3010980.1"
                     /translation="MLQRRALALALGLAAAPAAHGAPADAPLRVVTTSTDLKSLVEAV
                     GGARVEAESLAPALQDPHAVEVKPGQIARLKRATLLVRVGLDHEPWLPKALAAAGEPR
                     FARGGPAEVDASRGVRLIQTETPRLRADARPHVHGLGNTHYWLDPENARPITASILDA
                     LGRASPSERAQFEANRRRFLERLDAGLERWQRALGPWRGTQVVVVHETWPYFAARFGL
                     KIVAAAEPTPGVPPTPAELAALIAHMRASQVRIVVADPYSSPFLTRQLAERAGARVVT
                     LVPSVGGDPAASDYIALFDVNVKRMREALAAP"
     gene            complement(3343..4425)
                     /locus_tag="AC16CX_00300"
     CDS             complement(3343..4425)
                     /locus_tag="AC16CX_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3010981.1"
                     /translation="MCRSHVKNKLVLAVAFAVFAAGVCAPVAAQKGRHHSLTATLETT
                     QWGWLDPKESPKLTVNSGDTVSIETMMHAHNKVQQGITMDEIVALRKANPGGGPHSVT
                     GPIYVNGAEPGDVLEIRILKIVPKSFGINFNLPGKDFPTIGALASEMPDGFVKYFSLE
                     PGKNQAEFKPGVMIDLQPFPGTLAVGIDPDDPSPRKGGSKDPLAPVSTLRPWKNGSNM
                     DLNELRAGSTLYVPVFLKGGLIWAGDSHCRQGNGEVNLTGLECSFREIQIQPIVRKDM
                     KLSWPRAENKTHWITMGFDEDLNKAMVSATREAVNLLTEQKRVPLNRYEAYSIVSMVG
                     DCRVTQVVDIRKGVHCMIPKSIFTKK"
     gene            complement(4680..5123)
                     /gene="cynS"
                     /locus_tag="AC16CX_00305"
     CDS             complement(4680..5123)
                     /gene="cynS"
                     /locus_tag="AC16CX_00305"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HIR3010982.1"
                     /translation="MTRADVTELVVMARIKKGLTWAKIAKAVGQSKEWTTAALLGQMQ
                     MTKEQAEVAGKLLGLPEDAVLLLQQVPYKGSLPTAVPTDPLIYRFYELVNVYGTTLKA
                     LIHEEFGDGIMSAIDFKMDLTREPDPKGDRVKIVMSGKYLPYKTY"
     gene            complement(5290..6213)
                     /locus_tag="AC16CX_00310"
     CDS             complement(5290..6213)
                     /locus_tag="AC16CX_00310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3010983.1"
                     /translation="MSDPLIRVEGITKCFAARDGAVSTVFENVHFSVEQGEFVCLIGH
                     SGCGKTTILNILAGLEPASDGYVFVGGREIRGPSLDRAVIFQSHALMPWLTALGNIAF
                     AVTSRWPEWDRARVRSHCQKYIDLVNLTGAERKRPAELSGGMKQRVGIARALAIQPKM
                     LLMDEPFSALDALTRGMLQEEALRICAETRQTVFMITHDVDESILLADKIVLMTNGPD
                     ARIAEIVVNTLPRNRSHHTLHKEPHYYPIRNHLVDFLVSRSRQFNAEQPADYDPKHPP
                     LVTPGLSEAIPSIAHAAPRPAEKAVVVNLRH"
     gene            complement(6271..7113)
                     /gene="ntrB"
                     /locus_tag="AC16CX_00315"
     CDS             complement(6271..7113)
                     /gene="ntrB"
                     /locus_tag="AC16CX_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HIR3010984.1"
                     /translation="MKGIQYWGAPLLSVVLLLVFLAVWHAGTRQEAGAQVVDAEYAKL
                     VGAEAATGKKSAFPTPAEVGRKLWGHLTDPFYDKGPNDKGIGIQLAYSIARVLAGYLL
                     AALVAIPIGFVIGMSPLLYRAFDPFIQVLKPISPLAWMPLALFTLQDSGVSAVFVIFI
                     CSMWPMLINTAFGVASVRREWLNVAKTLEVGPVRKAFRVILPAAAPTIMTGMRISIGI
                     AWLVIVAAEMLVGGTGIGYFVWNEWNNLSIANILNGILAIGLVGMILDQVLARLTRLV
                     TFPE"
     gene            complement(7263..8636)
                     /locus_tag="AC16CX_00320"
     CDS             complement(7263..8636)
                     /locus_tag="AC16CX_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3010985.1"
                     /translation="MSAFKDPFNPEVKLGHGCSCGHHKDQAEHDADSARAAGNTETLA
                     ARVVDQAVMRALFPSDSERRRFLKAVGANTALAAVAQLFPLALAREVFAQGKGQIEKP
                     SLKVGFIPITCATPIIMSQPMGFFGRAGLNVDVVKTAGWAVIRDKTLNKEYDAAHMLS
                     PMPLAITLGVGSKAIPYTMPAVENINGQAITLSVKHKDKRDPKQWKGFKFAVPFDYSM
                     HNYLLRYYVAEHGLDPDKDIQIRAVPPPEMVANLRADNIDGFLGPDPFNQRAVYDGVG
                     FIHILTKELWEGHPCCAFAASKEFVTQYPNTYRTLLKAIMEATAFAAKPENRKQIAEA
                     IAPANYLNQPVTVIEQVLTGTFADGLGNVRKEPNRIDFDPFPWHSFAIWILTQMKRWG
                     QLKGDVDYAKVAREVYLATDAARLMKEVGMKPPASTLKKLTVMGKEFDPAKPDAYLAS
                     FAIKRAA"
     gene            8874..10325
                     /locus_tag="AC16CX_00325"
     CDS             8874..10325
                     /locus_tag="AC16CX_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3010986.1"
                     /translation="MTPTAPTRIAALLVAAALANCAQNPVTGNPNLVLVSESQEIAIG
                     RREDTNVRKQYGAYDNGPLQQYVNEVGQRLAKASHRPAIEYRFLVVDSPEVNAFALPG
                     GYIYITRGILAYLNSEAELAAVLGHELGHVTARHSVQQLTAATAANVGASILQIFVPQ
                     TRGVVGDNVINLLGGALLSGYGREHELEADRLGAEYLARTGYDPQAMVKVVGVLKNQE
                     SFDAEVAKAEGRQPRAYHGLFATHPDNDTRLQQVVGEAARFKQASTRTAQEEFLRRAD
                     RMTFGDSARQGIVRDSHFYHAELGLALRFPGGWRIQNRPQNVIATSPGGETYIDLRAG
                     GPAQGSPEEVLRKRVSAGFGTQVTQTTIGGLPAAIMNTSIGGRPTRVACVFLGKTAYL
                     VGSQARTSAAFSQHEGVIQSSLTSFHAITEQERALARPLRLRVITAQAGLTFSELARR
                     SQLGRFAEGHLRVINGLYPKGEPVAGQPLKIIE"
     gene            10487..>11619
                     /locus_tag="AC16CX_00330"
     CDS             10487..>11619
                     /locus_tag="AC16CX_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR3010987.1"
                     /translation="MISQRSLLVALLCAAWILPGLIGHDPWKPDEAHTFGVVYEMLRG
                     GSWVVPMLAGEPFLDKPPLFYLTAAASARLFSFVLPLHDAARLATGLWMAATFAFIAL
                     AGRELYGMRYGAVSALLLLGCFGFVVRAHMLITDVALLAGFAMVYYGLAAALKRPLLG
                     GFWTGTGIGVGFLANGLLAPIVLGATALLLPALGRDWRSRGYAVALAVAAAAAAPWLA
                     IWPLLLYQHAPAFFQDWLWGENLRYYFGAGAGTFYYLKILPWYAFPVWLLALWTLWRA
                     RAAGLARPAIVLPITGFVLTLVVLSASSEARELYALPLLLPLSLLAVAAPETLRRGAT
                     NAWYWFSAMTFTFFIAVFWFYWSGLELGVPARLHEHLHRIRPG"
BASE COUNT         1905 a         3857 c         3863 g         1994 t
ORIGIN      
        1 cagcgcgcgg ccctgccgcg atcgacgtct gccgccagct cgcggatctt tcccggggcg
       61 agcagcgccc gcgggtcgac ggtcatgtag gcgggatcgg tgatgcaatg gtcgcgcacg
      121 ccgaaagcgc gcttggtcgc ctcgaccgcg agatgcacgt agtccgcgct cgccggcccc
      181 gtggagccga cctccagcgc gtcgagcatg cccaggatca gcagcgatac caggccctgg
      241 gtcggcggcg gcaggttgta gaccgtgccg agcgagtgtt cgagcgcgag cggggcgacg
      301 aatttcgcac gatggcggcc gaggtcatcg agcgcgagcg ggcttcccgc ggcggcgaga
      361 tcccgcgcga tcgcccgggc gagcttgccg cggtagaaat cctcgagccc ggcgcgggcg
      421 aggcgggcga gcgtcgccgc gagccgcggc tgcttgaaca ggctgccctc gggcgaccct
      481 cctccggcga gaaacgtttc cctgaagccg ggctgcggct cgagctcgcc gcgcttggcg
      541 gcggtcgtgg acgactggct cctcgtgacc gcgatgccgt gctccgcgta atgaatcgcg
      601 tccgcgagca ggcgcttgag cggcagcttg ccgccccact tgcgcgagag cctgaacgca
      661 agctcccagc ctgaaacggc gcccgcgacc gtgttcgcgg cggcgccgcc gcgatagggg
      721 atcgcccgcg tgatgccgcg cgccgcgtac cactcgatgg aagcggcgcg ggcggccgcc
      781 ccgcaggcgt cgatgccgcg cggcggccgg cgcggaacct ggacgagcca gaacgagtcg
      841 ccgccgaccg aattcatgtg cggataggcg acggtgatcg tcgcggcggc ggcgagcatc
      901 gcctcgagcg cgttgccgct ctcgcgcagt acgtcgagcg ctgactgcgc ggcgagggcg
      961 tgcggcgcga ctgccatgcc gcgcgtggcg cgtatcgaat tgatcatgaa ataggattaa
     1021 cgaaaaaagt tgtcatttta tggcagcgcg ggttgaactg cggcttgccg tccgttcaaa
     1081 cccgcgcaga taaaacggct gctgcggctg cctttattat ttttttcgca gtggcgcggc
     1141 ccgtctcagc ataatcagca gcaatccgag cgacaccagc agcagcggca cgccgagcca
     1201 gtaacgctgg cgctcgcggg ctttcgcgcg cagcttgcgc agcgcgagct gatcgcccgc
     1261 cgagatctca ctgcgcccag caatgtactg gcgcagcctt tcctcctggt cggcgtccat
     1321 cgtgcgggtg ccccagcgca cctcctgtgc tacggcacgc aagcgcgcca gctcggcttc
     1381 ggcccggatc accgattcgg ccgcgtcgcg tcgcgcttcg cgttcggccg gcgtaaggaa
     1441 ccattcttgg agccgtggtg ccgcatgttc cagggcgtcg agccagaggt gatccatgcg
     1501 cggaaatgcg acaagcagtg cgccacccaa tgccaccgct ccgcacagca ccacgagcgc
     1561 cggggcgagt gctgcggcac cgcgagcgcg gaccgcgcgc gacgcccggc gcagccccag
     1621 gccgagggcg acgagcgcga tcgccgcccc gaacgcactg acgaccgccg cgcccgtagg
     1681 caggtcccac gcccacgagg cggcgagccc tgcggcgctc acggcgccgc cgaccgtcca
     1741 gccgatccaa aggcgcgccg ccaccgtgcg cgcgagcagg gcgccgatcg cagcggggac
     1801 cacgaggtac gagaaaacca gcagcacgcc cgccacgcgc acggacgaag tgacgaccag
     1861 ggcgaaggtg gcgtaaaaaa gcatgtccca cagaagcacg tggcgtccgg ccgcggcggc
     1921 tggatcgagc gagagctgca gcatcgggcg gcgcacggcc cagtgcaggg caccgattgc
     1981 cgagtacagc acggcaagct ccagaacgtc attcgccgac accgacagta tcgaaccgac
     2041 caggagctgc ttgatgtgct cggcgccctg cggcgcgcgg tccacgacca gcactgcgag
     2101 cgccgcggac accgcgtata tgatgccgat gatcgcctcc tggggaacct tctcctcttg
     2161 cgcccgggtc aacgcaaata ggatgccgcc tccgatcgca aacgcgagcg cgtaccagta
     2221 cgcggcctcg ctgtgcacgg ggtgcccggc gaggatcgcc acggtaagac ctagcgctgc
     2281 gatctgagcc agagtcaggt caacgaagat cacaccccgg gcgaggacgt gaaagccgag
     2341 gtacacatgg atcgccgtga gcaccagaca cgcgaaaaag ggcagggcaa gcagctcgag
     2401 catgcggttt cagggcgcgg caagcgcctc cctcatgcgc ttcacgttga catcgaaaag
     2461 cgcaatgtaa tcgctcgccg caggatcgcc gccaacggaa ggcacgagcg ttaccacacg
     2521 ggcgccagca cgctccgcga gctggcgcgt cagaaagggg ctggagtacg gatcggcgac
     2581 gacgatccgc acttggctcg cccgcatgtg cgcgatgagg gcagcgagtt ccgccggcgt
     2641 gggcggcacg ccgggcgtag gctccgcagc ggcgacgatc ttcagcccga agcgtgccgc
     2701 gaagtacggc cacgtttcgt ggactacgac cacctgagtg ccgcgccagg gccctagcgc
     2761 gcgctgccag cgttccaagc cggcgtccag ccgctcaagg aagcgtcggc gattggcctc
     2821 gaactgcgcc cgttcggacg gacttgcacg tccgagtgca tcgagaatcg aggcggtaat
     2881 cgggcgcgcg ttttccgggt cgagccagta gtgggtgttg ccgaggccgt gtacgtgcgg
     2941 ccgcgcgtcc gcgcgcagcc ggggcgtctc ggtctggatc agccgcacgc cgcgtgaggc
     3001 gtccacttcc gccgggccgc cacgcgcgaa acgcggctcg ccggccgcgg cgagtgcctt
     3061 gggcagccac ggctcgtgat ccagccctac gcgcaccagc agcgtggcac gcttgagccg
     3121 cgcgatctgc ccaggtttta cctctaccgc gtgcggatcc tgcagcgcgg gcgcaaggct
     3181 ttcggcctcg acgcgggcgc cgcccaccgc ctcgacgagc gacttgaggt cggtggatgt
     3241 cgtgacgacc cgcagcggcg catcggccgg tgcgccgtgc gccgcaggag ccgcagcgag
     3301 gccgagggcg agcgcgagcg cgcgccgttg cagcaccgcc cgctacttct tggtgaagat
     3361 cgacttcgga atcatgcagt gaaccccctt gcggatgtcc accacctgcg ttacccggca
     3421 gtcccccacc attgagacga tcgagtacgc ctcgtagcga ttgagcggca ctctcttctg
     3481 ttcggtgagg aggttcaccg cttcgcgcgt tgcgctgacc atcgccttgt tcaggtcttc
     3541 gtcaaagccc atcgtaatcc agtgcgtctt attctcggcg cgcggccacg agagcttcat
     3601 gtccttgcgc acgatcggct ggatctggat ctcgcgaaac gagcactcga gtccagtcag
     3661 attcacctcg ccgttgccct ggcggcagtg cgagtcgccc gcccagatga ggccgccttt
     3721 caggaatacc ggcacgtaca gggtcgagcc tgcccgcagc tcgttcaggt ccatgttcga
     3781 gccgttcttc caggggcgca gcgtcgaaac gggcgccagc gggtctttcg agccgccttt
     3841 gcgcggcgag ggatcgtccg gatcgatgcc gaccgcgagc gtaccaggga aaggctgcag
     3901 atcaatcatg acgccgggct tgaactcggc ctgatttttc ccgggctcga gggagaagta
     3961 cttcacgaaa ccgtccggca tctcagatgc cagcgcgccg atggtgggga aatccttgcc
     4021 cggcaggttg aagttgatgc cgaagctctt gggcacgatc ttgaggatgc ggatctccag
     4081 cacgtcgccc ggctcggcgc cgttgacgta gatcggcccg gtcaccgaat gcgggccgcc
     4141 gccgggattc gccttgcgca gggcgacgat ctcgtccatg gtgatgccct gctgcacctt
     4201 gttgtgcgcg tgcatcatcg tctcgatcga gacggtgtcg cccgaattga cggtgagctt
     4261 gggcgactct ttggggtcga gccagcccca ttgggttgtc tccagcgtcg ccgtgagcga
     4321 gtggtggcgg cccttctggg cggcgacggg cgcgcagacg cctgcggcaa aaactgcgaa
     4381 tgcgacggcc aaaacgagct tgttcttgac atgggatctg cacatggctg tctcctcctt
     4441 tgggttagac gcgcctaatc gtcagccgcg cctgcgacgg tgtcaagggg ccatacaaga
     4501 aacacgcgcc cggcatgact tggcgcaaga accttctccc ggaaccggcc ggggatgcac
     4561 tgcaacatgc aacactcgtc gcataaaata aatcacgcat taagcggcag cacgctgtcc
     4621 gcttgaaagc gcgaaattaa agcggcgccg ggcgcatctt ggcgcgcccg cgtccgcctt
     4681 caataggtct tgtacggcag atacttcccg ctcatcacga tcttcacgcg gtcgcccttg
     4741 gggtcgggct cgcgcgtgag gtccatcttg aagtcgatcg cgctcatgat gccgtcgccg
     4801 aactcctcgt ggatcagggc cttcaaggtt gttccgtaca cgttcacgag ctcgtagaag
     4861 cggtagatca gcggatcggt cggcaccgcg gtgggcagcg agcccttgta cggaacctgc
     4921 tgcagcagca gcaccgcgtc ttcgggaagc ccgaggagct tgccggcgac ttccgcctgc
     4981 tccttcgtca tctgcatctg gccgagcagc gcggcggttg tccactcctt gctttggccc
     5041 accgccttcg cgatcttcgc ccaggtcagg cctttcttga tcctcgccat caccaccagc
     5101 tcggtgacgt cggcgcgggt catgggtttg ctcatgctcg cagcggcgag cagggcttcg
     5161 ggtgcgtcgg atttcttcat gctcactctc ctttgtaatg aacacagctg tctgcgctta
     5221 gcttgcagct atcagcttga accggggtga ctggctggaa gctgatggct gaccgccgat
     5281 tgctcaacat cagtgcctca agttgacgac gacggccttc tcggcgggtc gcggcgcggc
     5341 gtgtgcgatg ctcgggatcg cttcgctcaa acccggcgtg acgagcggcg ggtgcttcgg
     5401 gtcgtagtcc gccggctgct cagcgttgaa ctgcctcgat cggctcacca ggaaatccac
     5461 caggtggttg cgaatcgggt agtaatgcgg ctccttgtgc agggtgtggt gcgagcggtt
     5521 ccgcggcagc gtgttgacga cgatctcggc gatcctcgcg tcggggccgt tggtcatgag
     5581 aacgatcttg tcggcgagga ggatcgactc gtccacgtcg tgcgtgatca tgaacacggt
     5641 ctggcgcgtc tcggcgcaga ttctcagcgc ttcttcctgc agcatgccgc gcgtcagcgc
     5701 gtcgagcgcc gagaacggct cgtccatgag cagcatcttg ggctggatcg cgagcgcgcg
     5761 cgcgatcccc acgcgctgct tcatgccgcc ggagagctcg gccgggcgct tgcgctccgc
     5821 cccggtgagg ttcaccaggt cgatgtactt ctggcagtgg ctgcgcaccc gcgcccggtc
     5881 ccactcgggc cagcgcgagg tgacggcgaa cgcgatgttg ccgagcgcgg tcaaccaggg
     5941 catcagtgcg tggctctgga agatcaccgc gcgatcgagg ctcgggcccc ggatctcgcg
     6001 gccgccgacg aagacataac cgtcggaagc aggctcgagc ccggcgagga tgttgaggat
     6061 ggtggtcttg ccgcagccgg agtggccgat caggcagacg aactcgccct gctcgaccga
     6121 gaagtgcacg ttctcgaaca ccgtgctgac ggcgccgtcg cgcgcggcga agcacttggt
     6181 gatgccctcg actcggatca gggggtcgct cattgcgcga tcccggtgat gggtgatgag
     6241 tgatgggtga tgagcaactt caagctttcc tcactccggg aaggtcacca ggcgcgtcag
     6301 gcgcgcgagg acctggtcga ggatcatgcc gaccaggccg atcgcgagaa tgccgttcag
     6361 gatgttggcg atcgagagat tgttccactc gttccacacg aagtagccga tgccggtgcc
     6421 gccgaccagc atctcggccg cgacgatcac cagccacgcg atgccgatcg agatgcgcat
     6481 gcccgtcatg atggtgggcg cggccgccgg cagaatcacg cggaacgcct tgcgcacggg
     6541 ccccacttcc agcgttttcg cgacgttgag ccactctcgc cggacggacg cgacgccgaa
     6601 ggcggtgttg atcagcatcg gccacatcga gcagatgaag atcacgaaca ccgcggagac
     6661 gccgctgtcc tggagcgtga acagcgcgag cggcatccac gcgagcggcg agatcggttt
     6721 cagcacctgg ataaaggggt cgaacgcgcg gtagagaagc ggcgacatgc cgatcacgaa
     6781 cccgatcggg atcgccacca gcgccgccag caggtacccc gccagcacgc gcgcgatcga
     6841 gtaggcgagc tggatgccga tgcccttgtc gttggggccc ttgtcataga acgggtcggt
     6901 gaggtggccc cagagcttgc gtcccacctc ggcgggcgtg ggaaacgccg acttcttccc
     6961 ggtggcggct tccgcgccga cgagcttcgc gtactcggcg tccaccacct gcgcacccgc
     7021 ctcctgccgg gtacccgcgt gccagacggc gaggaaaacg agcagcagga cgaccgagag
     7081 cagcggcgcg ccccagtatt ggatgccctt cacgtggcgc tccgtgacga gtgacgagtg
     7141 acgggtgaca tagccttcat gctgccacct ctgcctggat tgtcgtcata taaaggacat
     7201 cgcgtcggtg gcccggcggc attcacggtc gcatccccgt aaacggggcc cctgctccac
     7261 gctcatgccg cccgcttgat ggcaaagctc gcgaggtagg cgtcgggctt ggccgggtcg
     7321 aactccttgc ccatgacggt cagcttcttg agcgtggaag ccggcggctt catgcccact
     7381 tccttcataa gccgcgcggc gtcggtcgcg agatagacct cgcgcgcgac cttcgcgtag
     7441 tccacgtcgc ccttcaactg gccccagcgc ttcatctggg tgaggatcca gatcgcgaac
     7501 gagtgccacg ggaagggatc gaaatcgatg cggttcggct ccttgcgcac gttgccgaga
     7561 ccgtcggcga aagtgccggt cagcacctgc tcgatcaccg tcaccggctg gttcaaataa
     7621 ttcgccgggg cgatggcttc cgcgatctgc ttgcggttct cgggcttggc cgcgaacgcg
     7681 gtcgcctcca tgatggcctt caacagcgtg cgataggtat tcggatactg ggtcacgaac
     7741 tccttgctcg ccgcgaacgc gcagcaggga tgcccttccc acagctcctt ggtgaggatg
     7801 tggataaagc cgacgccgtc gtacaccgcc cgctggttga agggatcggg cccgaggaag
     7861 ccgtcgatgt tgtcggcgcg caggttggcc accatctcgg gtggcggcac cgcgcggatt
     7921 tggatgtcct tgtccgggtc gaggccgtgc tcggcgacgt agtagcgcag caggtagttg
     7981 tgcatcgagt agtcgaacgg cacggcgaac ttgaaaccct tccattgctt ggggtcgcgc
     8041 ttgtccttgt gcttgacgga gagcgtgatc gcctggccgt tgatgttctc caccgcgggc
     8101 atggtgtaag gaatcgcctt cgagccgacg ccgagcgtga tggcgagcgg catcggcgag
     8161 agcatgtgcg cggcgtcgta ctccttgttg agcgttttgt cgcggatcac cgcccagccc
     8221 gcggtcttca ccacgtccac gttcagcccc gcgcgcccga agaagcccat cggctgcgac
     8281 atgatgatgg gggtggcgca ggtgatcggg atgaagccca ccttgagcga gggtttctcg
     8341 atctggcctt tgccctgcgc gaacacctcg cgcgccagag caagcgggaa gagctgagcg
     8401 accgccgcca gtgcagtgtt ggcgccgacc gctttcagga agcggcggcg ctccgagtcg
     8461 ctgggaaaca gcgcgcgcat tactgcctgg tccacgaccc gggcggcgag cgtttcggta
     8521 tttccggccg cgcgcgccga atcggcgtcg tgttcggcct gatccttgtg gtggccgcaa
     8581 ctgcagccgt gcccgagctt cacctcggga ttgaacggat ccttgaatgc ggacatgcgc
     8641 gtctcctgca gtggttgacg gttctcctac tgcacgatgc ataccagatc ggttcgcggc
     8701 gccgcgcgaa ccgctaaatc aaaccatcaa caacctgatt tgcctgaatt atcgataacc
     8761 gccgagatac acccattatt ctttccgcca aggcatcggc ggaaagaatg cacgttatta
     8821 gtgcggagcg caccacattc gtgcttcttt ttcttcgcgg gtaaaattcg ctcatgacac
     8881 cgactgcgcc cacgcgcatc gcggcgctgc tcgttgcggc tgcgctcgcc aactgcgcgc
     8941 agaaccccgt caccggcaac ccgaacctcg ttctggtctc cgagtcgcag gagatcgcca
     9001 tcggccggcg tgaagacacc aacgtcagga agcagtacgg ggcctacgac aacgggccgc
     9061 tgcagcagta cgtgaacgag gtcggccagc ggctcgccaa ggcgagccat cgccccgcca
     9121 tcgagtaccg cttcctggtc gtggactcgc ccgaggtgaa tgcgttcgcg ctgcccggcg
     9181 gctatatcta catcacccgc gggatcctcg cctatctcaa ctccgaggcc gagctcgcgg
     9241 cggtgctggg ccacgaactc gggcacgtca ccgcgcgcca ttcggtgcag cagctgaccg
     9301 cggccaccgc ggccaacgtc ggcgcctcga tcctgcagat tttcgtgccg cagacgcgcg
     9361 gcgtggtagg cgacaacgtg atcaatctgc tcggcggcgc gctgctctcg ggctacgggc
     9421 gcgagcacga gctcgaggcc gaccgcctcg gcgccgagta cctcgcgcgc accggttacg
     9481 atccccaggc gatggtgaaa gtggtcggcg tgctcaagaa ccaggagtcg ttcgacgcgg
     9541 aggtggcgaa ggccgaaggg cgccagccgc gcgcctacca cgggctgttc gccacgcatc
     9601 cggacaacga tacccggctg cagcaggtgg tgggcgaagc cgcgcgcttc aagcaggcct
     9661 cgacccgcac cgcgcaggag gaattcctgc gccgcgccga ccggatgact ttcggcgaca
     9721 gcgcgcgaca gggcatcgtg cgcgacagtc atttctatca cgcggagctc gggctcgccc
     9781 tgcgctttcc cgggggttgg cgcatccaga accgcccgca gaacgtgatc gccaccagcc
     9841 caggaggcga gacgtatata gacctgcgcg cgggtgggcc ggcccaaggc agtcccgagg
     9901 aggtattgcg caaacgcgtc agcgcggggt tcggcacaca agtgacgcag accacgatag
     9961 gcgggctccc cgccgcgatc atgaatacca gcatcggcgg ccgcccgacc cgcgtggcgt
    10021 gcgtgtttct gggcaagacc gcctatctcg tcggctcgca agcgcggacg agcgcggcat
    10081 tcagccagca cgaaggcgtg atccagtcgt cgctcacgag ctttcacgcc atcaccgagc
    10141 aggaacgggc gcttgcgcgc ccgctcaggc tgcgggtgat tacggcgcag gcgggactca
    10201 cattctcgga gctcgcgcgc cgctcgcagc tcggccgctt cgccgaaggg cacctgcgcg
    10261 tgatcaacgg cctgtatccg aagggcgagc cggtggcggg ccagccactc aagatcatcg
    10321 agtgacaatt gaaatacgtg ggtcgtggat tgtgggtcgt gggtcgagcc aggaccgcag
    10381 tagccgatcc acgaatcacg acccacgatc cacgcttttc acggcgttga tgcgataatc
    10441 gcacgcaaac gcgaatgcgc atcctccacc acaccgcagt tctgcaatga tctcgcagcg
    10501 cagcctgctc gtcgccctgc tctgcgcagc ctggatcctg cccggtctca ttggacacga
    10561 cccgtggaag ccggacgagg cccacacctt cggcgtggtg tacgagatgc tgcgcggcgg
    10621 ctcgtgggtg gtgccgatgc tcgcgggcga gccgttcctg gacaagccgc cgctcttcta
    10681 ccttaccgcc gccgcgtccg ccaggctgtt ctcgttcgtg ctgccgctgc acgacgccgc
    10741 ccggctcgcg accgggctct ggatggcggc cacgttcgcc ttcatcgctc tcgccgggcg
    10801 cgagctctac ggcatgcgct acggcgcggt ctccgcgctg ctgctgctcg gctgcttcgg
    10861 attcgtggtg cgggcccaca tgctcatcac cgacgtggcg ctgctcgccg gcttcgcgat
    10921 ggtctactac gggcttgcgg ccgcgctcaa acgcccgctt ctcggcgggt tctggaccgg
    10981 caccggcatc ggcgtcggct tcctcgccaa cggcctgctc gcgccgatcg tgctcggcgc
    11041 aaccgccctg ctgctcccgg cgctcgggcg cgactggcgc agccgtggtt atgccgtcgc
    11101 cctcgcagtc gccgcggccg cagccgcgcc gtggctcgcg atctggccgc tcctgctcta
    11161 ccagcacgcg cccgctttct tccaggactg gctatggggc gagaacctcc gctattactt
    11221 cggagcgggg gcgggcactt tctactatct gaagatcctg ccatggtacg cgttcccggt
    11281 atggctgctc gccctctgga cgctctggcg agcgcgagcg gccggtcttg cgcggccggc
    11341 gatcgtgctt ccgattaccg ggttcgtgct cacgctggtc gtgttgtcgg cctcgtcgga
    11401 agcgcgcgag ctctacgcgc tgccgctgct gctgccgctc tcgctgctgg cggtggcggc
    11461 gcctgagacg ctgcgccgcg gcgcaaccaa cgcctggtat tggttcagcg ccatgacgtt
    11521 caccttcttc atagcggtgt tctggttcta ctggagcggg ctcgagctcg gtgtgccggc
    11581 gcgcctgcac gagcacctgc accgcatccg gcccggtta
//
LOCUS       DBNGWZ010000014         3959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_127259, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000014 DBNGWZ010000000
VERSION     DBNGWZ010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_127259"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..588
                     /locus_tag="AC16CX_00335"
     CDS             217..588
                     /locus_tag="AC16CX_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3010988.1"
                     /translation="MAPTQANERALLVTLVLFGFSAFIAALALIIFLLYLLGEPSVRA
                     TFPSPDMISGLATAESIDQLRKVCVSIARSLEIANATSEEQSRFINKAGGGLLAFLFL
                     WGSLSAAGMFYVYAKLRRVAK"
     gene            complement(810..1307)
                     /locus_tag="AC16CX_00340"
     CDS             complement(810..1307)
                     /locus_tag="AC16CX_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR3010989.1"
                     /translation="MTTTKSTPAFEAKICAPHELAARLKSLKRPLVFTNGVFDLLHRG
                     HVAYLAQARALGASLLVAVNSDVSARKLGKGGDRPVNALEDRLALVAALEAVTLVTWF
                     EEATPLELILAAKPDVLVKGGDWKPEAIVGAAEMKSWGGAVRSIPLLAGRSTTALIER
                     IRGKT"
     gene            complement(1340..1906)
                     /locus_tag="AC16CX_00345"
     CDS             complement(1340..1906)
                     /locus_tag="AC16CX_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3010990.1"
                     /translation="MRHAIYLLLAILPLVQACAPLVVGGATAGGVMVAQDRRTVGTMT
                     EDEGIELKAAGRIGERFKDGVHVNVTSYNRTVLITGEVPDATAMAEVERIVRSVQNVK
                     GVHNELAVAGVSSFTARSNDAIITSKVKARFLDAGKFNALHVKVVTENGVVYLLGLVN
                     KQEAADATEIARTTSGVRKVVRMFEPID"
     gene            complement(1906..2514)
                     /locus_tag="AC16CX_00350"
     CDS             complement(1906..2514)
                     /locus_tag="AC16CX_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HIR3010991.1"
                     /translation="MQPARIVDLITRISENFSESAHLKLQAMDALAAPIAAAAEHMVQ
                     CLKNDGKIMSCGNGGSAADSQHFAAELVNRFEMERPALAAIALTTDTSTLTSIANDYD
                     YDQVFSKQVRALGRAGDLLLAISTSGNSRNVIAAMHAAHECQMDVIALTGRNGGKMAE
                     ALRPSDIHICVPAQSTARIQEVHILTLHCLCDAIDCLLLGVE"
     gene            complement(2656..2997)
                     /locus_tag="AC16CX_00355"
     CDS             complement(2656..2997)
                     /locus_tag="AC16CX_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR3010992.1"
                     /translation="MNRRGDPAESLAAEFLERRGLKILARNYRCRFGEIDLVAASGAA
                     LVFVEVRARESEAFGGAAASITAAKRRRLVAAARHYLAKHRVNRACRFDVVLIRGRER
                     EIEWVANAFGE"
     gene            complement(2994..>3959)
                     /locus_tag="AC16CX_00360"
     CDS             complement(2994..>3959)
                     /locus_tag="AC16CX_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HIR3010993.1"
                     /translation="PHIALLLPLNSKSFGKHAEAVRDGFLAAAKVQRGAALPVRTYAL
                     GDDPEQAVDIYVRALNAGARIVVGPLTRSGVTAVAASAAVLVPTLALNVPEGHFDLPP
                     EIYMLSLQVEAEARQVAQLAWQEGVRKVITINGDTPLLKRIHQAFVEEFTRLGGAHVA
                     AYAFTSDPEGLTRIKKAADTGTVDMGFLALDLARARLARPYLGALPVYATSQVHPGNA
                     GPLAGFDLAGVRFLDMPWLLQPDHPAVMVYPRPAFRDGIEFDRFYALGIDAFRIALAL
                     LAGKADAALDGVTGRLTLGRDRQFARGLTAAQFTDGKLTVVRERP"
BASE COUNT          641 a         1276 c         1280 g          762 t
ORIGIN      
        1 gttctctggt ccataggtga cgtttggctc cacggcatgg gcacctcctt cggtgcccga
       61 taatgtgtgc aaactgttac ctatgtcccc cgactatact gttacctatg tgtccagacc
      121 gtaccaaatc acatgcctaa ccacccgatc cacaagccga cgcccgcagg cgggcgcctg
      181 ctcgcgcggg tgatcgggac acgttagaac gcataaatgg ctcccaccca ggcaaacgag
      241 cgggcacttc tggtaacgct ggtgttattc gggttttctg cgttcatcgc ggccctggca
      301 ttaataattt tcctgctgta tctcctcggc gagccttctg tgcgagcgac ctttcccagt
      361 ccggatatga tttcgggtct cgcgacggct gagagtatcg atcaactgcg taaggtgtgc
      421 gtttcaatag cccgcagcct cgaaatagcc aatgccacca gcgaggaaca gtcccgcttc
      481 attaacaaag ccggcggtgg gctccttgca ttcctctttc tttggggcag tctttccgcg
      541 gcaggcatgt tctacgtata cgccaagttg cggcgtgtgg cgaaatgacg ttgcgttcta
      601 actatgcgat cgaccgcgac gccggaaggc actacgcgcg tccgggcgcg cgtcatcgcg
      661 gaagttaagc cgtccttttt gaatttcctg acgtgcttgg cggccttggc ggtttattct
      721 agttcttgat tttggtgttg cttctctctg cgttctcggc ggttcgatta ttgttcttgt
      781 atttccttgg cgttctccgc ggtgaggttt taagtcttcc cgcggatgcg ctcgatcaat
      841 gccgtcgtcg agcgcccggc cagaagcgga atcgagcgca cggcgccgcc ccagctcttc
      901 atttcggccg cgccgacgat cgcttccggc ttccagtcgc cgcctttcac cagcacgtcg
      961 ggtttcgcgg cgaggatcaa ctcgagcggc gtcgcctcct cgaaccaggt gacgagcgtc
     1021 accgcctcga gcgccgccac cagagcgagc cggtcttcca gcgcgttgac cggccggtcc
     1081 ccgcccttgc cgagctttct tgccgaaacg tcggaattga ccgcgaccag caggctcgcg
     1141 cccagcgcgc gcgcctgcgc gaggtaggcg acgtgcccgc ggtgcaggag atcgaacacg
     1201 ccgttggtga acaccagcgg ccgcttgagc gatttcagcc gcgccgccag ttcatgcggt
     1261 gcgcagattt tggcctcaaa cgcgggagtg gacttggtgg tagtcaccag ccaccactca
     1321 cgggtcagta gtcacgattc taatcgatgg gctcgaacat ccgtactacc ttccgcacgc
     1381 cgctcgtggt acgcgcaatc tcagtggcat cggcggcctc ctgcttgttg accagcccga
     1441 ggaggtaaac gacgccgttt tcggtcacca ccttcacgtg cagcgcgttg aacttcccgg
     1501 cgtcgaggaa gcgcgctttc accttggagg tgatgatggc gtcgttgctg cgcgcggtga
     1561 acgagctcac gccggcgacg gcgagctcat tgtgtacgcc cttcacgttt tgcacactgc
     1621 gcacgatgcg ttctacttcc gccatggcgg tcgcatcggg cacttccccg gtaatcagca
     1681 ccgtgcggtt gtagctcgtc acgttgacgt gcacgccgtc cttgaagcgc tcgccgatgc
     1741 gaccggcggc tttcagctcg atgccctcat cctcggtcat ggttcccacc gtgcggcggt
     1801 cctgggcgac catgacccca ccggcagtcg caccgccgac cacgagcggc gcgcacgctt
     1861 gaacgagcgg caggatggca agcagcaggt aaatcgcgtg acgcatcact caactcccag
     1921 taacagacaa tcgattgcgt cgcacaggca gtgcaacgtc aggatgtgaa cttcctggat
     1981 gcgcgcggtg ctttgcgcgg gaacgcagat gtggatgtcg gagggccgca gcgcctcggc
     2041 catcttgccg ccgttgcgcc ccgttagcgc gatcacgtcc atctggcatt cgtgggcggc
     2101 gtgcatcgcc gcgatcacgt tgcgcgaatt tccgctggtc gagatggcga gcaggagatc
     2161 cccggcgcgc cccagcgcgc gcacctgctt ggagaacacc tggtcgtagt cgtagtcgtt
     2221 ggcgatggag gtgagggtgg atgtatccgt ggtgagcgcg atcgccgcga gcgccggccg
     2281 ctccatctcg aagcggttga cgagctcggc agcgaaatgc tgcgagtcgg cggcggagcc
     2341 gccgttgccg cagctcatga ttttcccgtc gttcttcagg cattgcacca tgtgctcggc
     2401 cgccgccgcg atcggcgcgg cgagcgcgtc catcgcctgc agtttgaggt gtgccgactc
     2461 cgaaaagttc tcgctgatgc gcgtgatgag gtccacgatg cgtgccggtt gcaagactcc
     2521 cgctattcta ccgacctatc gggcgctgcg taactgcgtt acgctgctcg accctcaccc
     2581 ccgcccctct cccgagaaga gaggggagag tcgagcgaac ccggattgtc atccggtttc
     2641 gtggatatta atgggtcatt caccaaacgc gttggccacc cattcgattt cccgttcgcg
     2701 cccccggatg agcacgacgt cgaaacggca ggcgcggttc acccggtgct tcgcgagata
     2761 atgtcgggcc gcggcgacca gccgccggcg cttggcggcg gtgatgctcg cggcggcgcc
     2821 gccgaacgcc tccgactcgc gggcgcgcac ctcgacgaac accagggccg cgccgctcgc
     2881 cgcgacgagg tcgatctcgc cgaaacggca gcggtagttg cgcgcgagga tcttcaatcc
     2941 gcggcgctcg aggaactcgg cggcgaggct ctcggcggga tcgccgcggc ggttcatggg
     3001 cgctcgcgca ccaccgtgag cttgccgtcg gtgaactgcg cggcggtgag gccccgggcg
     3061 aactggcgat cgcggcccag cgtgagccgc ccggtcacgc cgtcaagagc ggcgtcggcc
     3121 ttgcccgcga gcagcgcgag cgcgatccgg aaagcgtcaa tgccgagcgc gtagaagcgg
     3181 tcgaactcga tgccgtcgcg gaacgccggc cgcggataga ccatcaccgc aggatggtcg
     3241 ggctggagca gccacggcat gtcgagaaag cgcacgccgg cgagatcgaa tccggcgagc
     3301 gggccggcgt tgccgggatg cacttgcgac gtggcgtaga ccgggagtgc gccgaggtag
     3361 gggcgggcga gtcgcgcgcg cgccagatcg agggcgagaa atcccatgtc gaccgtgccg
     3421 gtgtccgcgg cctttttgat gcgggtgagg ccttcggggt cgctggtaaa cgcgtaagcg
     3481 gccacgtgcg cgccgccgag gcgcgtgaat tcctcgacga acgcctggtg gatgcgcttg
     3541 agcagagggg tgtcgccgtt gatggtgatc accttgcgga caccctcctg ccaggcgagt
     3601 tgcgccacct gccgcgcttc ggcttcgacc tgcaggctca gcatgtagat ctcgggcggc
     3661 aggtcgaaat gtccctccgg cacgttgagc gcgagcgtgg gcaccagcac cgccgcgctc
     3721 gcggcgaccg cggtcacgcc gctgcgcgtg agcggtccga caacgatccg cgcgccggca
     3781 ttgagcgctc tgacgtagat atccacggcc tgctccggat cgtcgccgag cgcgtacgtg
     3841 cgcaccggca gcgccgcgcc gcgctgtacc ttggcggccg cgagaaagcc atccctgacg
     3901 gcttcggcgt gcttcccgaa tgacttcgag ttgagcggca gcagcagcgc aatgtgcgg
//
LOCUS       DBNGWZ010000015         7992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1134669, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000015 DBNGWZ010000000
VERSION     DBNGWZ010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1134669"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC16CX_00365"
     CDS             <1..392
                     /locus_tag="AC16CX_00365"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR3010994.1"
                     /translation="FEMARGGRVDTVILGAYEVDQTANVANWSTADAKRGGIGGAMDL
                     LSGKGDLVIVMEHTDSKGRPKLRKQCTYPLTGKGCVTHVVTDLALLRWDGKRFVLDEV
                     APGFTPQEVIALTEMEIVAAPDVRAMA"
     gene            complement(401..718)
                     /locus_tag="AC16CX_00370"
     CDS             complement(401..718)
                     /locus_tag="AC16CX_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3010995.1"
                     /translation="MKQIHIAKHAPEAHLIKGFLESNGIVAVVRGEFLTSGWGELPVD
                     VCSVWIDDDRQYERAQALLVAFLRGDFAREFRDQNWRCPKCGEQLEGQFTACWKCGTR
                     RPA"
     gene            917..1624
                     /locus_tag="AC16CX_00375"
     CDS             917..1624
                     /locus_tag="AC16CX_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3010996.1"
                     /translation="MAVIRILSAGAAQAVVEKIAAAYTRDTGHKVEADFSAVGAMKAR
                     VVAGEPVDIIVLTGALIDELAASGHVAPGSRADLGKVGTGVAARAGTPTPDISSAPAL
                     RGSLLAATKIAFPDPETATAGKVVMQVFERLGIAEQVRPKMQFFRNGYAAMQWLAASR
                     GQLEIGITQITEILANPGVTYAGPLPAGLQTKTIYAAGLAVRAGEPGAAREFIGRLTS
                     PAARGVLAKAGYEFDSD"
     gene            1735..2127
                     /locus_tag="AC16CX_00380"
     CDS             1735..2127
                     /locus_tag="AC16CX_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3010997.1"
                     /translation="MNRIVHLALKVENLEKATEFYEKVFGFREVETRKTRDHISRHMT
                     DGKLDFTLMKYDEGATSRESLAAGEKPCIHHFAIEVDDIGARAADLQKYGCEIISDPG
                     VIPMKFRAPDGIVAEIVPARRYKKLKKK"
     gene            2215..2670
                     /locus_tag="AC16CX_00385"
     CDS             2215..2670
                     /locus_tag="AC16CX_00385"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3010998.1"
                     /translation="MAKTGDIISGAVLAGLGAFIISEARGWEYTGPDGPGPGFFPLWY
                     GVCMIALSALLVATSLAQRAREPGAAVNWREIGRALVAWFGLALCVGLLKVLGFLLAF
                     GLFTFFLVVVMYRRPLGTALAVSVGMSVGFYLLFPLALNVALPVGKLGF"
     gene            2794..4296
                     /locus_tag="AC16CX_00390"
     CDS             2794..4296
                     /locus_tag="AC16CX_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3010999.1"
                     /translation="MEGIGYLLHGFAVALSPINLAWCFVGVFLGTVIGVLPGLGPAAT
                     IAMLLPLTFKMDPTGAIIMLAGIYYGSKYGGSTTSILLNVPGESASVVTCFDGYAMAK
                     RGRAGAALGIAAIASFIAGTMGVVGLMLIAPPLAKFALSFSSPEYFALMCLGLAMVVL
                     LAGQSMVKALLAMLVGLWLTGIGTDLFTATSRFTFGNAELLDGVDFVVVAIGVFALGE
                     VLGNMESRESGQILPVPKGLRNLLPNAEELKACRFAFVNGSVIGFLIGVLPGAGSTIA
                     SFISYGVEKAFSKHPEKFGTGVPEGVAAPEGANNSETGGAMVPLLTLGIPGSGTTAIL
                     LAAFVLWGLRPGPLMIQDNPTLFWGLVASMYIGNVMLLVLNLPLVPLFAQILRLPAYV
                     LYPVIFGISIVGVYSVSSSLFDAWMLTAFGLLGYAMRKLDYPAAPLILGFVLGDSMER
                     ALRQSLMMSQGDLSILVSRPISATMLFLAVLVLVAPLFKKANAWRVKAIS"
     gene            4412..5383
                     /locus_tag="AC16CX_00395"
     CDS             4412..5383
                     /locus_tag="AC16CX_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011000.1"
                     /translation="MKSTSFVVALVLAAIASPIAAQGFKPGKPIEFVTHTGPGGGGDV
                     LARAISTAMEKEKLMPVRMQVVNKTGGGGATAMAYLAEKKGETHTIAVYTGLWFTNPI
                     MREEARIALKDLTPLVRLVLEPAMLVVKNDAPYKTLKDFIDAAKQNPGQLKQSAGSIG
                     ARDWLVRQLLMKHTGANWAYISFPGGGERIAALLGGHVNIMLIEPQEAGEHIRAGNLR
                     VLAQVSEKRLAAFPNVPTIKESGFDIPNVPQVRGIVAPPGIPAEAVAYWDDLFAKFVK
                     TASWRKILEDSLFEDGFMKSAELSKFIDQYSEMTRGILREGGVKVVR"
     gene            5637..6113
                     /locus_tag="AC16CX_00400"
     CDS             5637..6113
                     /locus_tag="AC16CX_00400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3011001.1"
                     /translation="MWLFLKNLVFILLVPGTVAGYVPWLLVRDKAFVPSAWTLLAAAT
                     FLVGVVMYLWCVWDFAVLGRGTPAPIDAPKKLVIRGLYQYVRNPMYLGVLTIILGWAI
                     HFHAVEVVVYGVGIGAAFHLFVVFYEEPHLEKTFGPAYEEYRSRVNRWIPKIKRDQ"
     gene            6129..7793
                     /locus_tag="AC16CX_00405"
     CDS             6129..7793
                     /locus_tag="AC16CX_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011002.1"
                     /translation="MPFVQPPPELGNQYSADRALRSYLARALPADVRTEIEPGLAAMG
                     ELAGGELYRFQLADRENEPVLTQWDAWGNRIDRVEVTPLWREAERIAAEHGLVATAYE
                     RKHGMYSRVHQFALVYLFTPSNDLYSCPLAMTDGAARALIAAGNQALIECAIPHLTSR
                     DPRQFWTSGQWMTEAAGGSDVGLTETVARREGDRWRLYGRKWFTSASTSQMALTLARP
                     EGNPPGGKGLALFYLETRDAQGRPNGILVNRLKDKLGTRKVPTAELTLDGALARLVAG
                     TGDGVKNIAPMLNVTRTWNSVSACAFARRGLALARDYAYRRIAFGAPLAEKPLHLDTL
                     AGLEAEFLGALHLTFFVAELMGREESEKASEGQGKLLRVLTPIAKLLTGKQGVQILSE
                     VIESFGGAGYVEDTGLPMLLRDVQVLPIWEGTTNVLSLETLRALEAVGGLAVLKREIG
                     ILLREVREPDLVKISARVEAAVEKAEAWLAKAKDAELEAGARRFAMTLGRATELALLA
                     RHAQWSLEHEKDQRTRAAARRFSAHGVSLLTDFDAADSRRLALDEQ"
BASE COUNT         1447 a         2403 c         2614 g         1528 t
ORIGIN      
        1 cgttcgagat ggcgcgcggc ggcagggtcg acacggtgat tctcggagcg tacgaggtgg
       61 accagaccgc caatgtcgcc aactggagca cggcggacgc gaagcgcggc ggcatcggag
      121 gggcgatgga cctcctctcg ggcaagggcg atctcgtgat cgttatggag cacaccgaca
      181 gcaagggccg gccgaaactg cggaagcaat gtacctatcc cctgacgggg aaaggctgcg
      241 tcacccacgt cgtcaccgac ctggcgctgc tgcgctggga cggcaagcgg ttcgtcctcg
      301 atgaagtggc tccggggttc actccgcagg aagtaatcgc actcacggaa atggagatcg
      361 ttgccgcgcc ggacgttcgt gctatggcgt gatcccggca tcacgccgga cggcgagtgc
      421 cgcacttcca gcacgcggtg aactgacctt ccaactgctc tccgcacttc gggcagcgcc
      481 agttctggtc gcggaactcg cgcgcaaagt cgcctctcag gaatgcgact agcagcgcct
      541 gcgcgcgctc gtactgccgg tcgtcgtcaa tccataccga gcacacatcc accggcagct
      601 ccccccagcc gctcgtcagg aactcgccgc gtaccacggc cacgatcccg tttgactcga
      661 ggaagccctt gatgagatgc gcttcaggcg cgtgcttggc gatgtggatc tgcttcatga
      721 tgggttactt tacgcctccg cgtaaggctg cgcacgtgtg ctagcatccc gctccatgcc
      781 gagtgctgag ttacaagttg caagtttcaa gctccaagct taacgtcctt tcccttgtaa
      841 cttgcagctt gtagcttgct actgtccgga ccgatccacg acccacgatc cacgaaattt
      901 ggggccgaac ggaaagatgg ccgttatccg aatattgagc gcgggagcgg cgcaggcggt
      961 ggtggagaag atcgccgcgg cctacacgcg cgacaccggc cacaaagtcg aggccgattt
     1021 cagcgcggtg ggcgcgatga aagcgcgggt cgtcgcgggc gagccggtgg acatcatcgt
     1081 tctcacaggc gcgctgatcg acgagcttgc agcctccggc cacgtcgcgc cgggctcgcg
     1141 cgccgacctg ggcaaggtcg gcacgggcgt agcggcgcgg gcgggcacgc cgacgccgga
     1201 tatttcaagc gcgccggcgc tgcgcggcag cctgcttgcc gcgacgaaga tcgcctttcc
     1261 cgacccggaa acggcgaccg cgggcaaagt tgtgatgcag gtgtttgagc ggctcggcat
     1321 tgcggagcag gtgaggccaa agatgcagtt tttccgtaat ggttatgcgg cgatgcaatg
     1381 gctggcggcg agccggggcc agctggaaat cggcatcacc cagattaccg aaatcctggc
     1441 aaaccccggt gtaacatatg cgggtccgct gcccgcgggt ctgcagacga aaacgattta
     1501 tgcggcgggg ctggcggtgc gggccgggga accgggtgcg gcgcgggaat ttatcgggcg
     1561 gctgacctcc ccggcggcgc gcggcgtgtt ggcgaaagcg ggctacgaat ttgacagtga
     1621 ttaagtaact aagtgattga gtgattaagt gggcgagaca gcgcgagggc agtgtgcacg
     1681 gttgctcaat cacttagtca cccagttact taatcacttt ccgaaggaaa ttcgatgaat
     1741 cgcatcgtgc atttggcgtt gaaggtcgaa aacctcgaga aggccaccga attctacgag
     1801 aaggtgttcg gtttccggga agtggagacg cgcaagacac gcgatcacat ctcgcgccat
     1861 atgaccgacg ggaagctcga cttcacgctc atgaagtacg acgaaggcgc gacgtccagg
     1921 gaatcgctgg cggcgggcga gaagccgtgc atccatcatt tcgcgatcga ggtggatgac
     1981 atcggcgcgc gcgccgccga tctgcagaag tacggctgcg agatcatctc ggacccgggc
     2041 gtcataccca tgaagttccg cgcgcccgac ggcatcgtcg ccgagatcgt tccggcccgc
     2101 cgctacaaga agctcaaaaa gaaatagctg tcagcagtca gctatcagcc aagccgctga
     2161 ggacgttcgt tggtagctga ccgctgacgg ctgaaggctg agagctaatg gctgatggct
     2221 aaaacgggcg acatcatatc cggcgccgtg ctggcgggac tcggcgcatt catcatttcc
     2281 gaggcgcggg gctgggaata caccggtccg gacgggccgg gaccggggtt cttccccctt
     2341 tggtacggcg tgtgcatgat cgcgctctcg gcgctgctgg tggcgacgag ccttgcgcag
     2401 cgcgcccgcg agcccggcgc cgccgtcaac tggcgtgaga tcggcagggc tctcgtggcg
     2461 tggtttggtc tcgccttgtg cgtgggcctg ctcaaagtcc tgggcttcct gctggcattc
     2521 ggcttgttca cctttttcct ggtcgtggtg atgtatcgcc gtccgctcgg caccgccctt
     2581 gccgtttcgg tgggcatgtc ggtgggtttt tatctcctgt tcccgttagc tttaaatgtg
     2641 gcattgcccg tcggcaagct cggcttctga aaagccgtga atagtgaatg gtgaatagtg
     2701 attggtgatc gcgctcatgc agaccgggtt taaccactca ccactcacca ctcaccaatc
     2761 acgcctttca aacgaacgaa gtgagtcggc ctgatggaag gcatcggcta cctgctgcat
     2821 ggcttcgcgg tcgcgctttc gccgatcaat ctggcgtggt gcttcgtcgg cgtctttctc
     2881 ggcaccgtca tcggcgtgct gccggggctg ggtccggcag cgacgatcgc gatgctgctg
     2941 ccgctcacct tcaagatgga cccgaccggc gcaatcatca tgctcgccgg catctactac
     3001 ggctccaagt acggcggatc gaccacctcg atcctgctca acgtgccggg tgagtctgca
     3061 tccgtcgtga cctgtttcga cggctatgcg atggcgaagc gcgggcgtgc cggtgccgca
     3121 ctcggaatcg ccgcgattgc ctctttcatc gccggcacga tgggtgtggt gggcctgatg
     3181 ctgatcgccc cgccgctcgc gaaattcgcg ctttccttca gctcgcccga gtatttcgcg
     3241 ctgatgtgcc tggggcttgc gatggtggtg ctgcttgccg gccagtcgat ggtcaaggcg
     3301 ctgctcgcga tgctggtcgg cctgtggctc acgggaatcg gcaccgattt gttcactgcc
     3361 acatcgcgat ttacgttcgg caacgccgag ttgctcgacg gtgtcgattt cgtggtggtg
     3421 gcgatcgggg ttttcgctct gggcgaggtg ctaggcaaca tggaaagccg cgaaagcggg
     3481 cagatcctgc cagtgccgaa gggtttgcgc aatctgctgc ccaatgccga agagctcaag
     3541 gcctgccgct tcgcgttcgt gaacggatcc gtgatcggtt tcctgatcgg cgtattaccc
     3601 ggcgcgggct cgacgatcgc ctccttcatc tcctacggcg tcgagaaggc gttctcgaag
     3661 caccccgaga aattcggcac cggcgtgccg gagggcgttg cagcgcccga aggcgccaac
     3721 aattcggaga cgggaggcgc catggtgccg ctgttgacgc tgggaatccc ggggtccggc
     3781 acgacggcga tcctgctcgc agcgttcgtc ctttgggggc tcaggcccgg tccgctgatg
     3841 atccaggaca acccgacatt gttctggggg ctggtcgcca gcatgtatat cggcaacgtc
     3901 atgctgctcg tattgaacct gccgctggtt ccgctgttcg cacagatcct gcgcctgccg
     3961 gcctacgttc tctaccccgt gatcttcggc atctccatcg tcggcgtcta cagcgtgtcg
     4021 agcagcctgt tcgatgcctg gatgctcaca gcattcggcc tgctcggcta cgcgatgcga
     4081 aaactcgatt accccgcggc gccgctgatc ctggggttcg tgctcggcga cagcatggaa
     4141 cgcgcgctgc gccagtcgct gatgatgtcg cagggcgacc tctcgatact ggtgtctaga
     4201 ccgatttcag ctacgatgct atttttggca gtgctggtac tggttgcgcc gctgttcaag
     4261 aaggccaatg cctggcgcgt caaggcgatc agttagagct agtgattaag tgactaggtg
     4321 attaggtgat taagttggac ctgataattg tcgttactca gtcacttaat tactcaatca
     4381 cttagtcacc cataattgcg gaggaggaag catgaaatca acatcattcg tggttgctct
     4441 tgtgttggcg gcaattgcat cgccaatcgc cgctcagggc ttcaagccgg gcaaaccgat
     4501 cgagttcgtc acccacaccg gtcccggcgg cggcggggac gtgctggcgc gcgccatttc
     4561 caccgcgatg gagaaggaga agttgatgcc ggtgcgcatg caggtcgtca acaagaccgg
     4621 cggtggaggg gcgaccgcga tggcctatct cgcggaaaag aagggcgaaa cccacaccat
     4681 agcggtgtac accggcctgt ggttcaccaa cccgatcatg agggaggaag ccaggatcgc
     4741 gctgaaggac ctcacgccgc tggtgcggtt ggtgctggag ccggcgatgc tggtcgtgaa
     4801 aaacgatgct ccgtacaaga cgctcaagga cttcatcgac gcggccaagc agaaccccgg
     4861 gcagctcaag cagtcggccg gctcgattgg cgcccgtgac tggctcgtgc gccagttgct
     4921 gatgaagcat accggcgcga actgggccta tatctcgttc ccgggtggtg gcgagcgcat
     4981 cgcggcgctg ctcggcggtc acgtcaatat catgctgatc gagccgcagg aagcgggcga
     5041 gcacatccgc gcgggcaatt tgcgcgtgct tgcgcaggtt tcggaaaaac gtctggctgc
     5101 tttccccaac gtgccaacca tcaaggagtc gggtttcgat attccgaacg tgccgcaggt
     5161 gcgcggcatt gtcgcgccgc cgggaattcc cgccgaagcc gtcgcctact gggatgacct
     5221 gttcgcgaag ttcgtaaaga cggcgagctg gaggaaaatt ctcgaggata gcctcttcga
     5281 agacggcttc atgaagagcg ccgagctgag caagttcata gaccagtact ccgagatgac
     5341 gcgcggcatc ctcagggaag gcggcgtcaa ggtcgtccga taaaagccgc gcatggccgc
     5401 gcggctttta tcaaaaggga tgagggcgga gggatgaggt acaagttaca agctccaaga
     5461 tttgcttccc ttcccttgaa ccggtctcaa aaatggagat agcacgccta accttccagt
     5521 tcttggtacg tgaatacgag ccatacgctc ccatttcgcg attattgaga ccggctcttg
     5581 taacttgcag cttgtaactt gtactgtctg agtcgaccca cgacccacgt cgtaacgtgt
     5641 ggctgtttct aaagaacctc gttttcatcc tgttggtgcc cgggacggtg gccggctacg
     5701 ttccatggct cctggtccgc gataaagcat tcgttccgtc ggcctggacc ctgctcgcag
     5761 cggcgacctt cctggtcggc gtagtgatgt acctgtggtg cgtatgggac tttgcggtgc
     5821 ttggccgcgg cacgccggcg ccgatcgacg ctccgaagaa gctggttatc cggggacttt
     5881 atcaatacgt tcgaaatccc atgtacctgg gagtcctgac catcatcctc ggctgggcga
     5941 tccacttcca cgcagtcgag gtcgtcgtat atggagtggg cattggagcg gcttttcatt
     6001 tgttcgtcgt gttctacgaa gagccccacc tcgagaaaac gttcgggccg gcctacgagg
     6061 agtaccggtc cagggtcaat cgctggattc cgaagatcaa gcgtgaccaa tagacgctaa
     6121 gatagcgcat gcccttcgtc cagccgccgc ccgagctcgg caaccagtac agcgctgacc
     6181 gcgcgctgcg ctcgtacctc gcgcgggcgc ttcccgccga cgtgcggacg gagatcgagc
     6241 ccggtctcgc tgcgatgggc gagcttgccg gaggcgagct ttatcgcttc cagctcgccg
     6301 atcgcgagaa cgagccggtg ctcacgcaat gggacgcgtg gggcaaccgg atcgaccgcg
     6361 tcgaagtgac gccgctgtgg cgcgaagcgg agcgcatcgc cgccgagcat gggctggtcg
     6421 ccaccgccta cgaacgcaag cacggcatgt actcccgcgt tcaccaattc gcgctggtct
     6481 atctcttcac gccttcgaac gacctctaca gttgcccgct cgcgatgacc gacggggcgg
     6541 cgcgggcact gatcgcagcc ggtaaccagg cgctgatcga gtgcgccatt ccgcatctca
     6601 cgagccgaga cccgcgccag ttctggacca gcggccagtg gatgaccgag gcggccggcg
     6661 gctcggatgt ggggctgacc gagaccgtcg cgcgccggga aggcgaccgc tggcggctct
     6721 acggccgcaa gtggttcacc tccgcgagca cctcccagat ggcgctgacg ctcgcccgtc
     6781 cggagggcaa cccgccggga ggcaaggggc tggcgctgtt ctatctcgag acccgtgacg
     6841 cgcagggccg gcccaacggc atcctcgtca atcgcctgaa ggacaagctg ggcacgcgca
     6901 aggtgccgac cgcggagctg acgctcgacg gcgcgctggc acggctcgtc gcgggcacgg
     6961 gggatggagt caagaacatc gcgccgatgc tcaacgtcac gcgcacctgg aactcggtga
     7021 gcgcttgcgc attcgcacgg cgcggcctcg cgctagcgcg cgattacgca tacaggcgca
     7081 tcgcgttcgg cgcgccgctt gcggagaagc cgctgcacct cgacacgctg gccggtctcg
     7141 aagcggaatt tctcggcgcg ctgcacctca cgttcttcgt cgccgagctg atggggcgcg
     7201 aggaatcgga gaaggcgagc gaagggcagg gaaagttgct gcgggtgctg acccccatcg
     7261 cgaagctcct caccggcaag cagggggtgc agatcctgtc ggaggtgatc gagtccttcg
     7321 gcggagccgg atacgtggag gatacggggc tgcccatgct gctgcgcgat gtgcaggtgc
     7381 tgccgatctg ggaaggcacg accaacgtgc tttcgctgga gacgctgcgc gcgctggaag
     7441 ccgttggcgg ccttgctgtg ctcaagcgcg agatcggcat cctgctgcgc gaagtgcgcg
     7501 agccggacct cgtgaagatc tcggcgcggg tcgaggcggc ggtggaaaaa gcggaggcgt
     7561 ggcttgcgaa ggcgaaggac gccgagctgg aagccggggc gcggcgcttc gccatgacgc
     7621 tggggcgcgc gacggagctg gcgctactcg cgcgccacgc gcagtggtcg ctggagcacg
     7681 aaaaggacca gcgcacgcgc gcggcggcgc ggcgattttc ggcgcatggc gtgagcctgc
     7741 taactgattt tgacgccgcc gactcgcggc ggctggcact cgacgagcag tgatcaaggg
     7801 aggaattgcg ggcatgaacg tagacgctaa ccgcattcga tgcgtcgatc ccctcgtcac
     7861 accgcaatac cccttcgacg acccgccagg gacacccgag cgatccgagc cccgctacgc
     7921 gacatttgcg gaccatgagg cccgcattcc gatgcgggac gggatcaggc tggccgccga
     7981 cgtgcttcgg cc
//
LOCUS       DBNGWZ010000016         3134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3149783, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000016 DBNGWZ010000000
VERSION     DBNGWZ010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3149783"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16CX_00410"
     CDS             <1..384
                     /locus_tag="AC16CX_00410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3011003.1"
                     /translation="TGFCWGGRLAWTYCVHNPKVKAGVAWYGRLVAPARAALQPAYPV
                     ELAPHLKVPVLGLYGGADASIPVEHVEQMRAALKNAGNTVNEIVVYDGAPHAFYADYR
                     PNYRKEAAEDGWKRMQAWFKKYGVA"
     gene            complement(423..1700)
                     /locus_tag="AC16CX_00415"
     CDS             complement(423..1700)
                     /locus_tag="AC16CX_00415"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3011004.1"
                     /translation="MYAPAAAQSRESPPGEPLQSAPVHREPHVAAIPRQSIMHYVDSI
                     TGARYSLGEPRWRGDSGGHVNLAEAPGLARQEIDTSMNSLWRYWRALLVDAADAVSMG
                     EGWTPLVDGDWDGVPVRFKLEFMMPTGSFKDRGMTVMVSYLKSRGVSEVLEDSSGNAG
                     ASLSAYCARAGMRCRILVPETASYPKIAQIAACGADVVTIRGSRQEVADAALAMSREI
                     FYASHNWQPFFAEGTKTLAYELWEQLGFHPPDNVVVPVGYGANVLGCDRGYSELLRNG
                     EIERMPRLYGVQAANCAPYYAAFCAGVEHFVPAPASPTVAEGIASSKPTRMREVLAPV
                     RTSGGEIVAVTEDEIVQALGALAKRGFYVEPTSAAAAAGLSQLIARGVIKKDETTVLV
                     LTGSGLKASDTIGKLLNLKARQGQPAMNRVRRS"
     gene            complement(1774..2100)
                     /locus_tag="AC16CX_00420"
     CDS             complement(1774..2100)
                     /locus_tag="AC16CX_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011005.1"
                     /translation="MSKLLTTLLAAAFAAATFGAAAQTKPAEPAKKAEPAKATPATPA
                     TPAKGDATKATPATPATPPAKADKPKVEKKAKKAKGEGKKKEAKPTKKRGEGKKKAPA
                     KEEMKK"
     gene            2295..3068
                     /locus_tag="AC16CX_00425"
     CDS             2295..3068
                     /locus_tag="AC16CX_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HIR3011006.1"
                     /translation="MDLELSGRTALITGGSRGIGFGVAQSLAAEGCHLHLSSRNAADL
                     EAARNKIVSAHKVNVTCHALDLAVSGNARKLGQACRDVDILVNNAGAIPQGTLTGLDE
                     KTWRESWDLKVFGYINLAREIYAAMCERRRGVIVNIIGGAGERPTAGYIAGSMANAGL
                     MAFSRALGAESPNHGVRVIGLNPSATATDRGVMRWRNQAQKDLGDPERWRELTKGFPF
                     GRPATVEEIADVVAFLCSDRASYVSGTVISVDGGAAARK"
BASE COUNT          507 a         1078 c         1003 g          546 t
ORIGIN      
        1 accggtttct gctggggcgg gcgcctcgcc tggacttact gcgtgcacaa cccgaaggtg
       61 aaagcgggcg tcgcctggta cgggcgactc gtcgcgcccg ccagggcggc gctgcagccc
      121 gcctaccccg tggagctcgc gccgcatctc aaggtgccgg tgctgggatt atacggcggg
      181 gcggacgcga gcatcccggt cgagcacgtc gagcagatgc gcgccgcgct gaagaacgcg
      241 ggcaacaccg tgaacgagat cgtcgtctac gacggcgcgc cgcacgcttt ctacgccgat
      301 taccggccga actaccgcaa ggaggcggcg gaagacggct ggaaacggat gcaggcgtgg
      361 ttcaagaaat acggcgtcgc ctgactaccg tgactcgtga ctggtgactc gtgacttggg
      421 ttttaacttc gtctgacgcg attcatcgct ggctgaccct gacgcgcctt taggttcaga
      481 agcttgccga tcgtatcgga cgccttcagg ccggagccag tcagcaccag cacggtggtt
      541 tcgtccttct tgatgacacc gcgggcgatg agctgagaca agccggcggc ggcagcagct
      601 gaagtcggct cgacgtagaa acctctcttt gcgagcgcgc cgagcgcttg cacgatctcg
      661 tcctcggtca ccgcgacgat ctcgccgccg gaggtgcgta ccggcgcgag cacttcgcgc
      721 atccgcgtcg gttttgacga cgcgatcccc tccgcgaccg tcgggctggc tggcgccggc
      781 acgaaatgct ccacacccgc gcaaaaggcg gcgtagtacg gcgcgcagtt tgcggcctgc
      841 accccgtaga ggcgcggcat ccgctcgatc tcgccgttgc gcagcagctc ggagtagccg
      901 cggtcgcagc caaggacgtt ggcgccgtag ccgaccggca ccaccacgtt atccggcggg
      961 tggaagccca gctgctccca cagctcatag gcgagcgtct tggtcccttc cgcgaagaat
     1021 ggctgccaat tatggctggc gtagaaaatc tcccggctca tggcgagcgc ggcatcggcc
     1081 acttcctggc gcgagccccg gatcgtcacc acgtccgcac cgcacgcggc gatctgcgcg
     1141 atcttcggat acgacgccgt ttccggtacg aggatacggc accgcattcc cgccctcgcg
     1201 caataggccg aaagggaggc accggcgttg cccgacgaat cctccagcac ttcactgacg
     1261 ccgcggctct tgagataact caccatcacc gtcatgccgc ggtccttgaa ggaaccggtc
     1321 ggcatcatga attcgagctt gaaccggacc gggacgccgt cccagtcgcc atcgaccagc
     1381 ggcgtccacc cttcccccat cgacaccgcg tccgcggcat ccaccagcag cgcccgccag
     1441 tagcgccaca gcgagttcat cgaggtgtcg atctcttgac gggcgaggcc tggcgcctcc
     1501 gccaagttga cgtggccgcc agaatcgccg cgccagcgcg gctcgccgag tgagtagcgc
     1561 gcgccggtta tcgaatcgac atagtgcatg atggattgcc tggggatcgc cgcgacatgg
     1621 ggctcgcgat ggacaggagc agattgtagc ggctcgccgg gcgggctctc ccgcgattgt
     1681 gccgcggcgg gcgcgtacaa agaaaaaggc ggcttgcgcc gcctttttcg atccgtccgg
     1741 cgagcctacg ccgtcggaac caaaggccca ccgttacttc ttcatctctt ccttcgccgg
     1801 ggcctttttc ttgccttcgc cgcgcttctt ggtcggcttg gcttccttct tcttgccttc
     1861 gcccttggcc ttcttggcct tcttttcaac cttgggcttg tcggccttgg ccggcggcgt
     1921 tgccggggtg gccggcgtcg ccttggttgc atcgcccttg gccggggtcg ctggcgtagc
     1981 cggggtcgcc ttcgcgggtt ccgccttctt cgcaggttcc gccggcttgg tctgcgccgc
     2041 agcgccgaag gtggccgccg caaacgccgc ggcaagcagg gtggtcaaca gtttgctcat
     2101 cttttctcct ttcctcgagt acgggtatgc cggaaatagc tccggtcagg ggctataacg
     2161 cacgaaccgg ccggcggttg acccgtccct gacgcccctc ccggcaggtg tttcggcagg
     2221 gatacagtag aatatcgcgc ccgccgaccc ggcggccaaa taccccctat caacccgcac
     2281 gacaatccaa cgtcatggac ctcgaacttt ccggtcgtac cgccctgatc accggcggct
     2341 cgcgcggcat cggcttcggc gtggctcaat cgctcgccgc cgaaggctgc catcttcatc
     2401 tctcctcgcg caacgccgcc gacctggaag cggcgcgcaa caagatcgtg tcggcgcaca
     2461 aggtcaacgt cacctgccac gcgctcgacc tcgccgtgag cgggaacgcg aggaagctcg
     2521 ggcaggcctg ccgcgacgtc gacatcctgg tgaacaacgc cggcgcgatt ccgcagggca
     2581 cgctcaccgg tctcgacgaa aaaacgtggc gcgaatcgtg ggacctcaag gtgttcggct
     2641 atatcaacct cgcgcgcgag atctatgcgg cgatgtgcga gcggcgccgc ggcgttatcg
     2701 tcaacatcat cggcggcgcc ggcgagcggc ccaccgccgg ctacatcgcc ggcagcatgg
     2761 cgaacgcggg gctgatggcg ttctcgcgcg cgctcggggc ggaaagcccc aatcacggcg
     2821 tgcgggtgat cggcttgaat cccagcgcca ccgcgaccga ccgcggggtc atgcgctggc
     2881 gcaaccaggc gcagaaggac ctgggcgacc cggagcgctg gcgcgagctg accaagggct
     2941 tcccgttcgg cagaccggcc accgtggagg aaatcgcaga cgtcgtcgcc ttcctctgct
     3001 ccgaccgcgc ttcctacgtt tcaggcacgg tcatcagcgt cgacggcggc gccgccgccc
     3061 gcaagtgatc ttgatcgggg cgatccgggg acgccgcggc cggcgctaat cctctccctc
     3121 caccgtcagc gaag
//
LOCUS       DBNGWZ010000017        10439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2898184, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000017 DBNGWZ010000000
VERSION     DBNGWZ010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2898184"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="AC16CX_00430"
     CDS             complement(<1..1745)
                     /locus_tag="AC16CX_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3011007.1"
                     /translation="MQSPLQSVLILLATAVLVVVVFRTLKLPPMLGYLIVGVAIGPHA
                     LAWIPDTQDARGLAEIGVVFLMFSIGLEFSLPRLVTMRRIVFGLGAAQVLATLLIVWG
                     ATAALGVEWRAGLMLGGVLAMSSTAILAKMLTERLELNSPHGRQIIGILLFQDLAVVP
                     LLILAPALAAGTDDLAAKLGLALAKAAVVLALLLFLGQRLMRGWFHLVAKQKSAELFV
                     LNVLLITLGLAYITDVAGLSLALGAFVAGALISETEYRYQVEVDIKPFRDVLLGLFFV
                     TIGMLLDLRVVFEQAPWVLLLLAALLAVKALLIVGLCLAFGSEIAVALRTGLALAVCG
                     EFGFVLLARADSLALVESQVMQPVLAAMLLSMLAAPFIVERSGHIARSWDRSDWMHRA
                     MALHNIAVQSMAAEQHVVICGYGRSGQNLARLLEKESIPFIALDVDPQRIREAAAAGE
                     SVVFGDAARREVLVAAGLLRAKALAITYSDTASALRILGHVQELRPGLPVVVRTVDDA
                     DIDRLKRAGAAEVVAEIMEGSLMLASTALMLLGVPLNRVLRRIRETREQRYSLFRGFF
                     RGVTDETGGEDEAAQP"
     gene            1937..2935
                     /locus_tag="AC16CX_00435"
     CDS             1937..2935
                     /locus_tag="AC16CX_00435"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR3011008.1"
                     /translation="MAPGSASRASQESAALTMAREVLEIEAKAITDLIGRLDQHFAQA
                     VEMILNCRGRVVVGGIGKSGHIARKIASTLASTGTPAFFVHPAEASHGDLGMVTRDDV
                     FIALSNSGESDELLAIIPLLKRQGAKLVAMTGNSQSALAREADVHLYAGAEKEACPLN
                     LAPTASTTAALALGDALALALMQAKGFTRDEFAASHPGGMLGRKLLTHVRDVMRTGDN
                     APRIAHTATAIEGMLEMSRGRMGMTAVLDEGGRVIGIFTDGDLRRNLEKGVNLHSARI
                     TEVMTRGPRTIGPDKLAVEAVEIMERFKVNQLLVVDPEQKLLGALNMHDLFRAKVI"
     gene            3022..3549
                     /locus_tag="AC16CX_00440"
     CDS             3022..3549
                     /locus_tag="AC16CX_00440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR3011009.1"
                     /translation="MQDIYTRARNIRVAAFDVDGVLTDGSLYYTDSGEELKRFNVRDG
                     HGMKMLKDSGVTLAIITSRRSRCVEERSRNLGIDLLFQGAADKLAAFEELLGRCGVGA
                     AACAYVGDDVLDLPVMRRCGLAATVPDAPASVRRRAHFITHARGGEGAAREVCDLILH
                     AQGSAAARLAEYAEK"
     gene            3644..4240
                     /gene="lptC"
                     /locus_tag="AC16CX_00445"
     CDS             3644..4240
                     /gene="lptC"
                     /locus_tag="AC16CX_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR3011010.1"
                     /translation="MILDRLTTAAPLLLVGMLTALTFWLERMAQPPAPGLRASRHDPD
                     YFVDNLTAVSLNQAGTARYTLAAAKMVHYPDDDTTILTTPKFVSYDSAKAPVTITASE
                     AVVSANGQHVYFQEDVRVTRAAYQESSELVMRTSFLHLIPDGKIAKTDRAVTITDAAM
                     TVTAVGLEINNETRVIKLLSKVQGTYDPSKAQRRESKR"
     gene            4200..4862
                     /gene="lptA"
                     /locus_tag="AC16CX_00450"
     CDS             4200..4862
                     /gene="lptA"
                     /locus_tag="AC16CX_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR3011011.1"
                     /translation="MIRARLSGANPSVNCGCSRAATLGRTWVFCALTALAAPAFAEKA
                     DRDKPVNLEADRVTIDDAKQTALFEGNVVLTQGTLQIRGDRMEVRQDKDGFRHGVTWG
                     NLAYFRQKREGYDEYIEGWAERIEYDGHAETMQMFNRAQLRRGQDEVRGNYISYDSKT
                     EFFQVVGGGAKAAAPGNPEGRVRAVIQPKSKEKPPAAPPVSLKPAEAIAAPREEPGTR
                     LK"
     gene            4897..5658
                     /gene="lptB"
                     /locus_tag="AC16CX_00455"
     CDS             4897..5658
                     /gene="lptB"
                     /locus_tag="AC16CX_00455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR3011012.1"
                     /translation="MPAGSATATPATGNNELRAERLKKRYRARVVVKDVSLEVRSGEV
                     IGLLGPNGAGKTTCFYMMVGLVPMDGGELHLDGTRLTHMPMHRRARLGLSYLPQEASI
                     FRRLTVAENVQAVLELYNQDPDAIRDKLDSLLHDLGIGHLHASPAISLSGGERRRVEI
                     ARALATDPRFILLDEPFAGVDPIAVLDIQKIIGFLKARGIGVLITDHNVRETLGICDR
                     AYIINDGSVLAYGKPEEIVYNESVRKVYLGEHFRL"
     gene            5893..7326
                     /locus_tag="AC16CX_00460"
     CDS             5893..7326
                     /locus_tag="AC16CX_00460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR3011013.1"
                     /translation="MKHSLQLRLSQHLTLTPQLQQSIRLLQLSTLELNQELERILQEN
                     PLLEREDDIRERPVAAVETAPRVTDPAPAAEAPSPDSAPTDGESAEFSALDNAPAPAY
                     RDEAEDGEYQQVAADAPTLRAHLLAQLSLTNLSDRDCKLVTLLIDSLDEDGYLTQSLE
                     ELAAILPPELEVDLDELQIALKHLQNCEPTGVGARSLSECLTLQLSALPKDTPHLAEA
                     LETTRNHLEALAARDFTRLRKLLRCEDAALRSVQKLIIALNPKPGRNFSAEETRYVVS
                     DVIVKKVKGVWIASLNPDAMPRLRINRLYAEILQRNRNACSQQLTSQLQEAKWLIKNV
                     QQRFDTILRVSQAIVDRQRHFFEHGEVAMRPLVLREIAEALDLHESTVSRVTTQKFMH
                     TARGIFELKYFFGSHVTTEAGGAASSTAIRALIKQLVSAENARKPLSDGQISEILAQQ
                     GIMVARRTVAKYRESLQILPINLRKSL"
     gene            7350..7676
                     /gene="hpf"
                     /locus_tag="AC16CX_00465"
     CDS             7350..7676
                     /gene="hpf"
                     /locus_tag="AC16CX_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR3011014.1"
                     /translation="MNLNLTGHHLEITPAIRDYISSKLQRITHHFDHVIDVNVILSVE
                     KLRRKIEASVHVRGKDIFCENADADMYAAIDGLVDKLDRTIIKHKEKSLEHRHNGAAL
                     KRLARK"
     gene            7673..8227
                     /gene="ptsN"
                     /locus_tag="AC16CX_00470"
     CDS             7673..8227
                     /gene="ptsN"
                     /locus_tag="AC16CX_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR3011015.1"
                     /translation="MTPGAAAREAKSGAVPTTPLHPPMNLISKLLPLPNVMIDLDVSS
                     KKRVFEQVGLLFENNHHIARSQVFDSLFAREKLGSTGLGQGVAIPHGRIKGLKEALGA
                     LVRMKSPIPFDAPDGQAVALIFVLLVPDRATDVHLQILSELAQMFSDKAFRERLLAAP
                     GAPELHQLITQWQPHAAGQHSPVV"
     gene            8196..9128
                     /gene="hprK"
                     /locus_tag="AC16CX_00475"
     CDS             8196..9128
                     /gene="hprK"
                     /locus_tag="AC16CX_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR3011016.1"
                     /translation="MPQVSIARLFKDNREKLKLEWIAGRSGGAKELNSELTKDSSQGL
                     IGHLNFIHPNLIQVLGTSEIAYLDGLDAANCRSMLAKVATRELACFIVAGVERVPQPL
                     VEVAESAVTPLLRSPMASVELMWMLRPYLARELAESTTAHGVFLDVLGMGVLITGDSG
                     VGKSELALELISRGSGLIADDVVELYRIGPETVEGRCPPLLKDFLEVRGLGVLDIRTI
                     FGEAALRLRKNLKLIVNLGKPAARLERLPLNAGSERIMGVDIRKVTIPVAAGRNLAVL
                     TEAAVRNYVLQLRGFDSTQEFVARQSQQMKNGKQ"
     gene            9201..10037
                     /gene="rapZ"
                     /locus_tag="AC16CX_00480"
     CDS             9201..10037
                     /gene="rapZ"
                     /locus_tag="AC16CX_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR3011017.1"
                     /translation="MQMILITGLSGSGKSVALNVLEDSGYYYVDNLPAKLLPQTVGFL
                     AAAGHPRVAVSVELRDPQALAELPGQLADFRNRGHDVRTLFLEAKTDTLIKRFSETRR
                     RHPLSDGKLTLTECIERERELLADIADLAHRVDTSDLGPNILRNWVKDFVGLPHAGLT
                     LLFESFGFKHGVPLNADLVFDARCLPNPNYDPRLRPLTGRDRAVVEFLGSDPATRKMI
                     EDIRRFVADWLPAFDRDNRSYLTVAIGCTGGQHRSVYLIEALAGHFRDQAQVLVRHRE
                     LS"
     gene            10127..>10439
                     /locus_tag="AC16CX_00485"
     CDS             10127..>10439
                     /locus_tag="AC16CX_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR3011018.1"
                     /translation="MKGETFQEVHIFPLNTVLFPDGVLPLKVFEQRYLEMTKACLRDN
                     RPFGVCLIREGREVGAAALPEPVGCFAVIEHWDMPQLGVFHLVARGGGRFRIRDMRVA
                     PN"
BASE COUNT         1983 a         3493 c         3323 g         1640 t
ORIGIN      
        1 ggctgcgccg cctcgtcttc gccgcccgtc tcgtccgtga cgccgcggaa gaaacctcgg
       61 aacaagctgt agcgctgctc gcgggtctca cggatgcgcc gcagcacgcg attgagcggc
      121 acccccagca gcatgagcgc ggtcgaagcg agcataaggc ttccctccat gatctcggcg
      181 acgacttccg cggcgccggc gcgtttcagc cggtcgatgt cggcgtcgtc caccgtgcgc
      241 accaccaccg gcaatcccgg ccgcagctcc tgcacgtggc cgagtattct cagcgccgaa
      301 gcggtgtccg aataggtgat ggcgagcgct ttcgcgcgca gcagccccgc cgccaccagc
      361 acttcgcgcc gcgcggcgtc cccgaacacc acgctctcgc cggccgccgc cgcctcccgg
      421 atgcgctgcg gatccacgtc gagcgcgatg aacgggatgg actccttctc cagcaggcgc
      481 gccaggttct gcccgctgcg cccgtaaccg cagatcacca cgtgctgttc ggcagccatg
      541 gattgcacgg cgatgttgtg cagcgccatc gcgcgatgca tccagtcaga gcggtcccag
      601 ctgcgcgcga tgtgtccgct gcgctcgacg atgaagggcg ccgccagcat cgagagcagc
      661 attgcggcga gcaccggctg catgacctgg ctttcgacca gcgcgaggct gtcggcgcgc
      721 gcgagcagca cgaatccgaa ttcgccgcac accgcgagcg caagcccagt gcgcagggcg
      781 accgcaattt ccgatccgaa tgcgaggcac agtccgacga tgagcaacgc cttcaccgcc
      841 agcagcgcgg cgagcaagag cagcacccag ggtgcctgct cgaacaccac gcgcaggtcg
      901 agcagcatgc cgatggtgac gaagaacaat cccagcagca cgtcgcggaa cggcttaatg
      961 tccacctcca cctgatagcg gtactcggtc tccgagatca gcgcgccggc aacgaaagcg
     1021 cccagcgcga gcgagagccc cgcgacgtcg gtgatgtagg cgagaccgag cgtgatcagc
     1081 aacacgttca gcacgaacag ctccgccgac ttctgcttcg ccactagatg gaaccagccg
     1141 cgcatgagac gctggccaag aaagagcagc agcgcaagca ccacggccgc cttggcaagc
     1201 gccaatccca gcttggccgc gagatcatcg gtgccggccg cgagcgccgg cgcgagaatc
     1261 agcaacggca ccaccgccaa gtcctggaat agcaggatgc cgatgatctg ccgtccatgg
     1321 ggcgagttga gctccaatcg ctccgtgagc atcttggcga gaatcgccgt ggaagacatc
     1381 gccagcacgc cgcccagcat cagtcccgcg cgccattcga ctccgagcgc ggcggtggcc
     1441 ccccacacga tcagcagggt ggcgagcacc tgggccgcgc ccaggccgaa cacgatgcgg
     1501 cgcatggtga cgagcctggg caggctgaac tccagcccga tgctgaacat caggaacacc
     1561 acgccgattt cggccaaccc gcgcgcgtcc tgcgtgtcag gaatccaggc cagggcatgc
     1621 ggcccgatcg caacgccgac gatgaggtag ccgagcatgg gcgggagctt gagcgtgcgg
     1681 aacaccacca cgaccagcac cgcggtggcg agcaggatca ggaccgattg cagcggcgat
     1741 tgcacgggaa gctgtgaacg gtgaacagtg agcggtgagc ggtgagcggt aaagtcaaaa
     1801 ctgtggttgc gatctatccg cttaccgctt acggctgacc gcttaccgca gtgaatgatg
     1861 cctcatcgcg cgtgaattgc aaggcgcgcg gccgaggaaa agtgcgtgat tctgctatac
     1921 tttccacgca atgaagatgg cacccggcag cgccagccgc gcatcccagg agagcgctgc
     1981 actcacgatg gcacgcgaag tgctggagat cgaagccaag gccataaccg atctcatcgg
     2041 caggctcgat cagcacttcg cgcaggcggt ggaaatgatc ctcaactgcc gcgggcgcgt
     2101 ggtggtgggc ggcatcggca agtcggggca catcgcgcgc aagatcgctt ccaccctggc
     2161 gagcaccggc acccccgcgt ttttcgtcca tccggcggaa gcgagccacg gcgatctcgg
     2221 catggtgacg cgcgacgacg tgttcatcgc gctctccaat tccggcgaga gcgacgagct
     2281 gctggcgatc atcccgttgc tgaaacgcca gggcgcaaag ctcgtggcga tgaccggcaa
     2341 ttcccaatcc gcgcttgccc gcgaagccga cgtccacctc tatgccggcg cggaaaagga
     2401 agcctgcccg ctcaacctcg cgccgacggc gagcaccacg gcggcgctcg cgctgggcga
     2461 cgcgctggcg ctggcgctca tgcaggcaaa gggattcacg cgcgacgaat tcgcggcctc
     2521 gcaccccggg ggaatgctgg gacggaaact cctgacccat gtgcgcgatg tgatgcgcac
     2581 cggcgacaac gctccgcgca tcgctcacac cgccacggcc atcgagggca tgctcgaaat
     2641 gtcgcgcggc aggatgggca tgaccgccgt cctcgacgag ggcgggcgcg tcatcggcat
     2701 cttcaccgac ggcgacctgc gccgcaacct ggagaagggc gtgaatctgc actcggcccg
     2761 gatcacggag gtcatgacgc gcggaccgcg caccatcggg ccggacaagc tcgcggtcga
     2821 ggcggtcgaa atcatggagc gtttcaaagt caaccagctg ctggtggtgg atccggaaca
     2881 gaagctgctc ggcgcgctga acatgcacga cctgttccgc gcaaaagtga tttgatagca
     2941 atcagctgtc agcgctcagc cagaatacct tgcgctgctg taagccgatg gctgaatgct
     3001 gatagctgaa agctgtgacc catgcaagac atctataccc gcgctcggaa catccgcgtc
     3061 gccgcgttcg atgtggatgg cgtgctgacc gacgggtcgc tttactacac cgactcggga
     3121 gaggagttga agcggttcaa cgttcgcgac ggccatggca tgaaaatgct caaggacagc
     3181 ggcgtgacgc tcgccatcat caccagccgc cgttcgcgct gcgtcgagga gcggtcgcgc
     3241 aatctgggca tcgatctgct gttccagggc gccgcggaca agctcgcggc gttcgaggag
     3301 ctgctcggcc gctgcggcgt cggggcggcg gcgtgtgcct acgtcggtga tgacgtcttg
     3361 gacctgccgg tgatgaggcg ctgcgggctg gcggcgacgg tccctgatgc accggcgtcg
     3421 gtacgccgcc gggcgcattt catcacccac gcccgcgggg gtgagggcgc cgcgcgcgag
     3481 gtttgcgatc tcatcctgca cgcccaggga tcggctgcgg cgcggcttgc tgaatacgcg
     3541 gagaaatgac aggcttctcg tcatttgcgg gcggcattgc ctcgggagct tgtccgaccc
     3601 aggtccggca aactcctgga accatgcccc caggcggttc ttcatgatat tggatcggct
     3661 gaccacggcg gcgccattgc tgctcgtggg gatgctgacg gcgctgacct tctggctgga
     3721 gcgcatggcg cagccccctg cgcccggcct gagggccagc cgccacgacc ccgactactt
     3781 cgtggacaat ctgactgcgg tcagcctgaa tcaggcggga acggcacgct atacactggc
     3841 ggcggcaaaa atggtgcatt atcccgacga cgacaccacg attttgacca cgccgaagtt
     3901 cgtcagctac gactcggcga aagcgccggt gacgatcacg gcgagcgagg cggtcgtttc
     3961 ggccaatggc cagcacgtgt acttccagga agacgtgcgc gtgacgcgcg ccgcctacca
     4021 ggagagcagc gagctcgtga tgcggacatc ctttttgcac ctgatacccg acggcaagat
     4081 cgcgaagacc gaccgggcgg tcaccattac cgacgcggcg atgacggtca cggcggtcgg
     4141 cctcgagatt aacaacgaaa cccgcgtcat caagctgctc tccaaggtac agggaaccta
     4201 tgatccgagc aaggctcagc ggcgcgaatc caagcgttaa ctgcggctgc tcccgcgccg
     4261 ccactctcgg ccggacgtgg gtcttctgcg cgctgacggc gcttgccgcg cctgcgttcg
     4321 cggagaaagc cgaccgcgac aagccggtca atctggaagc cgaccgcgtc accatcgacg
     4381 atgccaagca gaccgcgctc ttcgagggaa acgtcgtgct cacccagggc acgctgcaga
     4441 tccgcggcga ccgcatggag gtgcgccagg acaaggacgg cttcaggcac ggtgtgacgt
     4501 gggggaacct ggcctatttc cggcagaaac gcgaaggcta cgacgagtac atcgagggct
     4561 gggccgagcg catcgagtac gacggccacg ccgagaccat gcagatgttc aaccgcgccc
     4621 agctgcggcg cggccaggac gaggtgcggg gaaattatat ctcctacgac tccaagaccg
     4681 agttcttcca ggtggtcggc ggcggcgcca aggccgccgc gcccggcaat cccgagggcc
     4741 gggtgcgggc ggtgatccag cccaagtcga aggaaaagcc gccggccgct ccgcccgtga
     4801 gcctcaaacc cgccgaagcc atcgccgcgc cgcgtgaaga acccgggacc cgcctcaaat
     4861 aaggtcgttg acgacgccgg ccaggccgcg tccgcgctgc cagccgggtc cgcgaccgcc
     4921 actcccgcaa ccgggaacaa cgagttgcgc gcggagcgat tgaagaagcg ctaccgcgcg
     4981 cgcgtcgtgg tgaaggacgt ttcgctcgaa gtgcgcagcg gcgaggtgat cgggctgctg
     5041 gggccgaacg gggccggcaa gaccacctgc ttctacatga tggtggggct ggtgccgatg
     5101 gacggaggcg agctccacct cgacgggacg cggctcaccc acatgccgat gcaccgccgc
     5161 gcgcggctcg gcctgagtta cctgccgcag gaggcctcca tcttccggcg cctcacggtc
     5221 gccgagaacg ttcaggcggt gctggagctg tacaaccagg atcccgatgc gatccgcgac
     5281 aagctcgaca gcctgctgca cgacctcggc atcggccacc tgcatgcgag cccggcgatc
     5341 agcctctcgg ggggcgagcg gcgccgcgtg gaaatcgcgc gcgcgctggc gaccgatcca
     5401 cgcttcatcc tgctcgacga gccgtttgcc ggggtggacc cgattgccgt gctggacata
     5461 cagaaaatca tcgggtttct caaggcgcgc ggtatcggcg tgctgatcac cgaccacaat
     5521 gtgagagaga ctctcggcat ctgcgatcgc gcctatatca ttaacgacgg cagcgtgctc
     5581 gcctacggca agcccgagga aatcgtttat aatgaaagcg taagaaaggt ctatctggga
     5641 gagcatttcc gtctctagca gcctgtcgga ctggttccca ttgactgagc atgcggtgtt
     5701 aacaatcgct gctgatcgac gcttcggcga caggctgttt taggaaggaa aattcgagtg
     5761 tcgaaatgct ttgccggcca gaatcgactt cagtcgtctt gcctggttgc caataggcgg
     5821 tttacgttag gagtttgtcg tgacagagtc cgacaaactc ctgggtgaac acagcgacac
     5881 ccaccatcac gcatgaagca ttcccttcag ctccggctgt cccagcacct cacgctcacc
     5941 ccccaattgc agcagtccat ccggctgctg cagctctcca cgctggaact gaaccaggag
     6001 ctcgagcgca tactccagga aaacccgctg ctcgagcgcg aagacgatat ccgggagcgg
     6061 cccgtcgccg ccgtggagac ggcaccgcgc gtcacggatc ccgcacccgc cgcggaggcg
     6121 ccgtcaccgg attccgctcc gaccgatggc gagagcgccg aatttagcgc gttggacaac
     6181 gcgcccgcgc ccgcttaccg cgatgaggcc gaggacggcg aatatcagca ggtcgccgcg
     6241 gacgccccca cgctgcgggc gcatctgctg gcgcaactgt cgcttaccaa cctgtccgac
     6301 cgcgactgca agctggtcac cctgctgatc gattcgctcg atgaagacgg ctatctcacg
     6361 cagagcctgg aagagctcgc ggcgatcctg ccgccggaac tcgaggtcga tctcgacgag
     6421 ctgcagatcg cgctgaagca cctgcagaat tgcgagccta ccggcgtggg cgcgaggagc
     6481 ctcagtgagt gcctcacgct gcaactttcc gcgctgccca aggacacgcc gcatctagcc
     6541 gaagcgctgg agacgacgag aaaccacctc gaagcgctgg cggcgcgcga tttcacgcgg
     6601 ctgaggaagc tgctgcgctg cgaagatgcc gcgctgcgct cggtgcagaa gctgatcatc
     6661 gccctcaatc ccaagccggg gcgcaacttc tccgccgaag agacgcgcta cgtcgtctcg
     6721 gacgtcatcg tgaaaaaggt caaaggcgtg tggatcgcct cgctcaaccc ggatgcgatg
     6781 ccccggctgc ggatcaaccg gctctacgcg gaaatcctgc agcgcaaccg caatgcctgc
     6841 tcgcagcagc tcaccagcca gctgcaggag gcaaagtggc tgatcaagaa cgtgcagcag
     6901 cgcttcgaca cgatcctgcg ggtgtcgcag gcgatcgtgg accgccagcg gcacttcttc
     6961 gagcacggcg aggtggcgat gcgccccctg gtgctgaggg aaattgccga ggcgctcgat
     7021 ctccacgagt ccaccgtgtc gcgcgttacc acgcagaaat tcatgcacac cgcgcgcggt
     7081 attttcgagc tcaagtactt cttcggcagc cacgtcacca ccgaggccgg cggggcggca
     7141 tccagcaccg cgatccgggc gctgatcaag caactggttt cggcggagaa cgcgcgcaag
     7201 ccgctttccg acggccagat ttccgagatc ctggcccagc agggcatcat ggtcgcgcgc
     7261 cgcaccgtgg ccaagtaccg cgaatccctg cagatattgc ccatcaacct gagaaagagc
     7321 ctctagccta tagcctctag gagccccgca tgaacctgaa cctgaccggt catcatctcg
     7381 agatcacgcc ggccatccgc gactacatct cgagcaagct ccagcgcatc acccaccatt
     7441 tcgaccacgt catcgacgtc aacgtcattc tttccgtgga gaagctcagg cgcaagatcg
     7501 aggcgagcgt acacgtgcgc ggcaaggaca tcttctgcga aaacgcggat gccgacatgt
     7561 acgccgcgat cgacggcctg gtggacaagc tcgaccgcac catcatcaag cacaaggaaa
     7621 aatccctcga gcaccgccac aacggcgctg cgctcaagcg cctggccagg aaatgacgcc
     7681 gggcgccgcg gcgcgtgaag ccaagtcggg agccgtccca acaaccccat tgcatccgcc
     7741 catgaacctg atctccaagc tgctgccgct gccgaacgtc atgatcgacc tcgacgtgag
     7801 cagcaagaaa cgcgtgttcg agcaggtcgg gctgctgttc gaaaacaacc accacatcgc
     7861 gcgcagccag gtgttcgaca gcctgttcgc gcgcgagaag ctgggctcca ccgggctcgg
     7921 ccagggcgtc gcgatccccc acggccggat caagggcctg aaggaggcgc tcggcgcgct
     7981 agtgcgcatg aagagcccga tcccgtttga cgcgccggac ggccaggcgg tcgcgctgat
     8041 cttcgtgctg ctggtgcccg accgcgccac cgacgtgcat cttcagatcc tttccgagct
     8101 cgcgcagatg ttcagcgaca aggcattccg agagcggctg ctggcggccc ctggcgcgcc
     8161 ggagctgcac cagttgataa cccaatggca gccccatgcc gcaggtcagc atagcccggt
     8221 tgtttaagga caaccgtgag aagctcaagc tcgaatggat cgccgggcgc agcggcgggg
     8281 ccaaggaact caacagcgag ctcaccaagg attcctccca ggggctgatc ggtcacctca
     8341 acttcattca tcccaacctg atccaggtgt tggggacttc ggaaatcgcc tacctcgacg
     8401 ggctggacgc cgcgaactgc cgctctatgc tcgcgaaggt cgccacgcgg gagctcgcct
     8461 gtttcatcgt tgcgggcgtc gagcgagtgc cgcagccgct cgtcgaggtc gccgaatccg
     8521 cggtcacgcc ccttctccgc tcgccgatgg cgagcgtcga gctgatgtgg atgctgcgcc
     8581 cctatctcgc gcgggagctt gccgagagca ccaccgcgca cggggtcttc ctcgacgtgc
     8641 tgggcatggg tgtcctgatc accggggact cgggagtcgg caagagcgag ctcgcgctgg
     8701 agctgatcag ccgcggcagc ggcctgatcg ccgacgacgt ggtcgagctc taccgcatcg
     8761 gccccgaaac ggtggagggg cgctgcccgc ccctgctcaa ggatttcctc gaggtcaggg
     8821 gcctcggcgt gctcgacatc cgcaccatct tcggcgaggc ggcgctgcgc ctccgcaaga
     8881 acctgaagct gatcgtgaac ctgggcaagc cggcggcgcg gctggagcgg cttccgctca
     8941 acgccggttc cgagcgcatc atgggcgtgg acatccgcaa ggtcacgata cccgtggccg
     9001 ccggccgcaa tctcgcggtg ctcaccgagg cggcggtgcg caactacgtg ctccagttgc
     9061 gcggcttcga cagcacccag gaattcgtcg cccgccagtc ccagcagatg aagaacggta
     9121 agcagtaagc cgtgagcagt aagcggtaaa ctgccaatgc ttgcttctgt ccgctcactg
     9181 ctcaccgctc accgctcacg atgcaaatga tcctcatcac cggcctctcg ggctcgggca
     9241 agagtgtggc gctcaacgtg ctcgaggaca gcggctatta ctacgtggac aacctgcccg
     9301 cgaagctgct gccgcagacg gtgggtttcc ttgccgccgc cggtcatccg cgtgtggcgg
     9361 tgagcgtgga gctgcgcgat ccgcaagcgc tcgccgagct gcccgggcag ctcgcggact
     9421 tccgcaaccg cggacacgac gtgcggacgc tgttcctgga ggcgaaaacc gacacgctga
     9481 tcaagcgctt ctcggaaacg aggcggcgcc atcccctctc ggacggcaag ctcacgctca
     9541 ccgagtgcat cgagcgcgag cgcgaactgc tggccgacat cgcggacctc gcgcaccgcg
     9601 tggacaccag cgatctcggc cccaacatcc tgcgcaactg ggtcaaggat ttcgtggggc
     9661 tgccgcacgc gggcctcacc ctgctgttcg agtccttcgg cttcaagcac ggcgtgccgc
     9721 tcaacgccga tctcgtgttc gacgcgcgct gcctgcccaa tcccaattac gatccgcgcc
     9781 tgcgcccgct gaccggccgc gaccgggcgg tggtcgaatt cctggggtcc gacccggcga
     9841 cgcgaaaaat gatcgaggac atccggcgct tcgtcgccga ctggctgccc gccttcgacc
     9901 gcgacaaccg cagctatctc acggtcgcga tcggctgtac cggcggccag caccgctcgg
     9961 tctatctgat cgaggcgctc gccggccact tccgcgacca ggcgcaggtg ctggtgcgcc
    10021 accgagagct ttcgtgaggg tgtgagagcg ggagcgcgtg aggacgaagt agaatccatc
    10081 tcacgatctc ccgacctccc gatctcacga tccaaagcga attgacgtga agggcgagac
    10141 tttccaggaa gtacacatat tcccgctgaa caccgtcctg ttcccggacg gcgtgctgcc
    10201 gctcaaggtg ttcgagcagc gctacctcga gatgaccaag gcctgcctgc gcgacaaccg
    10261 cccgttcggc gtgtgcctga tccgggaggg acgcgaggtc ggcgccgccg cgctgccgga
    10321 accggtcggg tgcttcgccg tcatcgagca ctgggacatg ccccagctcg gggtattcca
    10381 cctggtcgcg cgcggcggcg ggcgattccg catccgcgac atgcgggtcg cccccaatc
//
LOCUS       DBNGWZ010000018         9252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3402011, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000018 DBNGWZ010000000
VERSION     DBNGWZ010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3402011"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /locus_tag="AC16CX_00490"
     CDS             <1..1551
                     /locus_tag="AC16CX_00490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3011019.1"
                     /translation="ERKRTEEALRESEERFRATFEQAAVGMALRGIDPRNPRWLRVNQ
                     KLADILGYTREELLRLTSMDITPLEDRDETISYNERLLRGKLTSYSREKRYVRKDGSI
                     IWANISLTAVRGADGRPAYVISVIEDITERKRVEQALRHSEQQYRYLFDANPMPVWVR
                     DVETNGFFAVNDAAVELYGYSREEFSRMTGYDLRSLEDSAAYSEFLRQRDPHENTVRR
                     WRHRKKDGSFIEVEVFSHGFTYEGRPARLALINDVTERVRVEEEVRRLNAELEHRVVE
                     RTRQLEAVNRELESFAYSVSHDLRAPLRSMDGFSQALEEDYGGRLDDTGRDYLRRARG
                     AAQRMTELVDDLLALSRVSIVGMRSDRVDLSSLAQEILEEMRQHEPQRKVDVKVEGGL
                     LVRGDPSLLRVAFHNLLDNAWKFTARKSDARIEVGVGKHDGKRAIFVRDNGAGFDMAY
                     AGKLFGAFQRLHGDADFPGTGIGLATVQRIIHRHGGEVWAEAAPDRGATFYLTLGEAP
                     AGAGTEGQ"
     gene            1613..2752
                     /locus_tag="AC16CX_00495"
     CDS             1613..2752
                     /locus_tag="AC16CX_00495"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR3011020.1"
                     /translation="MQAQSVMPSPLRVLIVEDSEDDALLIVRELRRGDYEPDFLRVDT
                     GEALERALRERNWDVIVSDFALPGFSGAAALELVKWRGLDVPFIIVSGAIGEETAVAA
                     MKAGAHDYVMKSALGRLVPAVRRGLAEAETRRSKRQSETALRQALHRLQALSSRLMRI
                     QETERRAVARELHDEIGQALTAVKINLQALLMHPGAPAQAGQVEESIRIVDRALQQVR
                     DLSLKLRPSQLDDLGLLAALRWHLDRQSQAGILDIQFSADPLPARLHPDVETACFRIV
                     QEALTNVMRHAGAKRVRIELRHRGDEIELAVEDDGHGFDVAAAQQRASAGESLGLASM
                     HERAALAGGRIELSSEPGGGARLRACFPLALAADEPVVQHERTGS"
     gene            2749..3402
                     /locus_tag="AC16CX_00500"
     CDS             2749..3402
                     /locus_tag="AC16CX_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3011021.1"
                     /translation="MKSLRLILADDHQLVRAGLRSLIGQMKNVEVVAEASSGHEVLSL
                     ASELHPDIVLMDITMPGMNGFDAARHLRKDFPGIRIIFLSMHASEEYVLQALRAGASG
                     YLLKDSAPLELELALQAVRWGETYLSPPISRQVVDSYMQRIGQSEQPLSMLTGRQREI
                     LQLIAEGNSTKDIATKLGLSVKTVESHRGQLMERLGIHDIAGLVRFAIRHGLVSPER"
     gene            3546..>9252
                     /locus_tag="AC16CX_00505"
     CDS             3546..>9252
                     /locus_tag="AC16CX_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3011022.1"
                     /translation="MGNITPHLDAVIVLNILMLEDDDEDAILIQRALRGTGYAHSLRR
                     ARNRAEFTAQLDAIPDLILSDFHLPDFDALGALDLLKKRGLDVPLILVSGLARDEEVL
                     AAMKRGAADFLSKDRLVRLGQAITATLEYRALRRQANEIQGALERSREELDFAFNHAA
                     TGMALTSLDARWLKVNPALCGIIGYVESELLATDCHSVTHPDDVAGDVELTRRLVAGE
                     VNSTQREKRYLHKLGHVVWVQVTASLVRGRDGTPRYRMLQVLDISNRKEAEERFRATF
                     NQAAVGIAHTDIEGRILEVNHKLCEIFGHPEAELLGRTTRELVHPDDRDRQDGLRLEL
                     LSGARRSFSGEKRCVRGDGTTIWVNRTVTLARQVISNVPYLIQVFEDITERKQTEKRL
                     ERASRARRVMVECNHILVHATDETQMLESMCRIVVESGGYKMAWVGLATGDPVRPVYP
                     AAHAGFENDAPMTGSAAWGADGRYRGFMSEVLASGEPHIARNILKDPTHAGRLARALQ
                     RGFQSSIALPLKGDGRILGAIAMYAAEPDAFDDEEIALLKELADDIAFGIVNLRTRIA
                     RRNAETALQESELRFRETFEQAAVGITRVDLNGVLVDVNQKFCDMLGYDKDELLGKSI
                     RDITHPADYGRGAQYRAQLAHGAMKSAAGEKRFIRKDGTALWARRTMSTACDDTGKPL
                     YVISVVEDISERKELERRFELTFNHAAVGMTQASLDGYLLQVNQKYADMLGYTREELR
                     GMSSEAITHPEDVSETLQTRKDLIEGRLESATSEKRLVRKDGRVIWARRSVSVARGTA
                     GEPLYFIAVVEEITDRKQIEIERARLAAIVDNSNDAIITRDLGRTVLTWNLAAEKLFG
                     WTAQEAIGQSIIMMVPPERRGENAQRRELVHRGLPVPAYDTVRLAKDGRRIDVSLSQS
                     AIKNEHGEVIAVSLVLHDITERKQAEAERARLAAIVESSADAIISRNLDGTILTWNAA
                     AEKMFGYAAAEVIGRDISFLIPPDRREEASQKRRILNEGRAVPTFDTVRVTEDGRGVD
                     VSLTLSPIKDPAGVMIGVSLVFRDISERRALERRYRETFDQAAVGLTRVDLNGVLVEC
                     NQKFCDMLGYSRGELLGKTIRDITHPDDYGQGAQYRADVVSGVAKSRAGEKRFLRKDG
                     TMMWARRTMSTACDETGKPQYVISVVEDITERKQAEERYRATFDNAPVGIMHTSTDDD
                     KILHANSKLCEMLGYTHDELVSLTTDQIIDPDHVGVDRTKYREKMLNGELDTFSSERL
                     YRRKNGSGFWVNRTVSLARDWASEPLYFIRIIEDISERKLVASRQAMEHAVTRVLAQS
                     ASIAEAMPAVLKTICEGLEWTCGIYWKWDEKAELLRCAEAWHREGEEVANFVTTSRQS
                     VNEAPAWHGKPLKIEAGGIVRRVWFGGVPVWYPDVSRHPDFRRGPLAARAGLHCAFAF
                     PILAGEQPLGVVEFYSRDIRQPDDVLLQIVRAIGSQIGQFIQRKEAEQALRQSEERYR
                     DLFEASPLPMWVWEDETLGFLAVNQAAIDHYGYSRDEFRRMNVRGIWASGEWERYEDS
                     LRDRDRKQNLSLQRRHRTKDGHIIDVEVTARRFVHGGRPVWLTLLNDVTERKRAEAAL
                     RESEEHLRAMFDQATVGITVSGLDLQYLQVNDKYCDIVGYSRDELLRMSVRDVNFSEY
                     MEDATKYREKLFRGEIASTVREKPLRRKDGTAVWVSMVTSIVRGNEGKPQQFISVIQD
                     ISERKRAEVALRQSEEQFQQLANNIPQVFWIADAGQKQTLYVSPAAETLIGRPLGEIQ
                     ASPRSLVRALHKEDRPRVRAARKDAAAGGYDQTYRIVRPDGSIRWVRDRAFPVHDDSG
                     AVHRIAGIAEDITDRKMAEERLLHLAHYDVLTSLPNRVLFYDRLRQALAQAKRNQWTV
                     GV"
BASE COUNT         1752 a         2901 c         3125 g         1474 t
ORIGIN      
        1 gagcgcaagc gcacggagga agcgctgcgc gaaagcgaag agcgcttccg cgccactttc
       61 gagcaggcgg cggtgggcat ggcgctgcgc ggcatcgatc cgcgcaaccc acgctggctg
      121 cgcgtgaacc agaagctcgc cgacatcctc ggctacacgc gcgaggagct gctgcgtctg
      181 acctccatgg acatcactcc tctagaggac cgcgacgaaa cgatcagtta caacgagcgg
      241 ctgttgcgcg gaaagctgac gagctactcg cgcgagaagc gttacgttcg caaggacggg
      301 agcatcatct gggccaacat ctcgctcacc gcggtgcgcg gtgcggacgg caggccggcc
      361 tacgtcattt ccgtgatcga ggacatcacc gagcgcaagc gggtggaaca ggccttgcgg
      421 catagcgaac aacaataccg ttatttgttc gacgccaatc cgatgccggt gtgggtgcgg
      481 gacgtcgaga ccaatggctt tttcgccgtc aatgacgcgg cggtggaatt gtacggttac
      541 tcgcgtgaag aattttccag gatgaccggc tatgatctca ggtcgctgga ggacagcgcg
      601 gcctattccg agttcctccg ccagcgtgac ccgcacgaaa ataccgtcag gcgctggcgc
      661 caccggaaaa aagacggcag cttcatcgaa gtcgaggttt tctcgcacgg cttcacctat
      721 gaagggcggc cggcgcggct ggcgctgatc aacgacgtca cggagcgggt gcgcgtcgag
      781 gaggaggtcc gcaggctcaa cgcggaactg gagcaccggg tagtcgaaag gacgcgtcag
      841 ctcgaagcgg tgaacagaga gctcgaatct ttcgcgtatt ccgtatcgca cgacttgcgc
      901 gcgccgctgc gcagcatgga cggcttcagc caggcgctgg aagaggacta tggcgggcgt
      961 ctggacgata ccggcaggga ctacctgcgg cgggcgcgcg gcgccgccca gcgcatgacc
     1021 gagctggtgg acgacctgct tgcgctgtcg cgcgtgtcga tagtcgggat gcgcagcgat
     1081 cgggtggatc tcagtagcct ggcacaggag atcctggaag agatgcgaca acatgaaccc
     1141 cagcgcaaag tcgatgtgaa ggtggagggt gggctgctcg tacgcggcga cccgagcctg
     1201 ctgcgcgtcg cgttccacaa cctgctggac aacgcgtgga aattcaccgc caggaagagc
     1261 gacgcccgaa tcgaggtcgg cgtcgggaaa cacgacggca agcgcgcgat tttcgtgcgc
     1321 gacaatggcg cgggattcga catggcgtac gccggcaagc tgtttggcgc attccagcgt
     1381 ctgcacggcg atgccgattt ccccggcacc ggcatcgggc tcgccacggt ccagcgcatc
     1441 atccatcgcc atggaggcga ggtgtgggcg gaagcggcgc cggatcgcgg ggcgaccttt
     1501 tacctcacgc tgggcgaggc cccggctgga gcggggacgg aaggacaatg agtgccggaa
     1561 ctggtgccat cctcccggcc cgcggccgtc ccggcttcga ggcgcgagtt tcatgcaggc
     1621 gcaaagcgtg atgccgtcac ccctgcgcgt tctgatagtg gaggacagcg aggacgacgc
     1681 gctgctcatc gtgcgcgagc tgaggcgggg cgactatgag ccggacttcc tgcgggtgga
     1741 taccggggag gccctggaac gggcgctgcg cgaacgcaat tgggacgtga tcgtttcgga
     1801 tttcgcgctg cccggcttca gcggcgcggc ggcgctcgag ctcgtcaagt ggcgtggcct
     1861 tgacgttccc ttcatcatcg tttcaggggc gatcggcgaa gagaccgcgg ttgccgcgat
     1921 gaaagccggc gcgcacgatt acgtgatgaa gtccgcactg ggacggctgg tgcccgcggt
     1981 gcgccggggg cttgccgaag cggaaacccg ccgcagcaag cgtcagtcgg aaaccgcgtt
     2041 gcggcaggcg ctccatcgcc tgcaggcgct gtcgagccgg ttgatgcgca tccaggaaac
     2101 cgaacggcgc gcggtcgcgc gcgagctgca cgacgagatc ggccaggccc tgaccgccgt
     2161 caagatcaac ctgcaggcgc tcctgatgca ccccggcgcg cctgcgcagg cggggcaggt
     2221 cgaggaaagc atccgcatcg tcgaccgggc gctgcagcag gtgcgggatc tttcgctcaa
     2281 gctgcggccg tcgcagctcg acgatctcgg gctgctcgcg gcgctgcgct ggcatctcga
     2341 ccgccaatcg caggcgggta tcctggacat ccaattcagc gccgatccgc tgcccgcgcg
     2401 cctgcatccg gacgtggaaa ccgcctgttt caggatcgtg caggaagcgc tgaccaacgt
     2461 catgcgtcac gccggcgcga agcgcgtgcg catcgagctt cggcaccggg gcgatgaaat
     2521 cgagctggcg gtggaggacg acggccacgg gttcgatgtc gccgcggccc agcagcgggc
     2581 ttccgcagga gaaagcctcg gcctggcgag tatgcacgag cgcgccgcgc ttgccggcgg
     2641 gcgcatcgag ttgtcctcgg aaccgggcgg gggtgcgcgg ctgcgggcct gtttcccgct
     2701 ggcgcttgcc gcggatgagc ccgtggtgca gcacgaacgg acggggtcgt gaagtccctg
     2761 cggctgattc ttgccgacga tcatcagctg gtgagggcgg gcctgcgctc gctcatcggg
     2821 cagatgaaga acgtcgaggt cgtggccgag gcgagcagcg ggcacgaagt gctctccctg
     2881 gcatccgagc tccacccgga catcgtgctg atggacatca cgatgccggg gatgaacggg
     2941 ttcgacgccg cccggcacct caggaaggac ttccccggga tcaggatcat tttcctgtcc
     3001 atgcacgcga gcgaggagta cgttctgcag gcgctgcggg caggggcctc gggctatctt
     3061 ctgaaggact cggcgccgct tgaactcgag ctcgccctgc aggctgtgag gtggggcgaa
     3121 acctatctca gcccgccgat atcgcgccag gtggtcgaca gctatatgca gcgcatcggg
     3181 cagagcgagc agcccctctc catgctgacc ggccgccagc gcgagatcct gcagctcatc
     3241 gcggagggca acagcaccaa ggacatcgcg acgaagctcg gtctcagcgt caagaccgtg
     3301 gagtcccatc gcggccagct catggagcgg ctcggcatcc acgatatcgc gggactggtg
     3361 cgtttcgcca tccggcacgg cctggtcagt ccggaacgat agtccgcgcc cttcggggtt
     3421 tcgtgtctga cgcttgaccc ggcccctgcc ggccgcaccc tctcggggat ttcccctgta
     3481 aaactcaggg tattcccgat tggctctgtc aatcaatggg ataagatgcg atagggagga
     3541 gagacatggg aaacattacc ccgcatctcg acgccgtcat cgtcctcaat atcctgatgc
     3601 tcgaggacga cgatgaggac gccatcctga tccagcgcgc ccttcgcggc accggctacg
     3661 cccattccct gaggcgcgcg cgcaaccgcg ccgagttcac ggctcagctc gatgcgattc
     3721 ccgacctgat cctttcggat ttccatctgc cggatttcga cgcgctcggc gcgctcgacc
     3781 tgctcaagaa acgcgggctc gacgtgccgc tcatactcgt gtcggggctg gcgcgggacg
     3841 aggaggtgct tgcagccatg aagcgcggcg ccgctgactt cctctcgaag gaccggctgg
     3901 tcaggctggg acaggcgatc accgccaccc tcgaataccg ggccctgcgg cgacaggcga
     3961 acgagatcca gggcgcgctg gagcgcagcc gcgaggagct cgactttgct ttcaatcacg
     4021 ccgccaccgg catggcgctc acctccctcg atgcccgctg gctcaaggtc aacccggcgt
     4081 tgtgcgggat catcggctac gttgaatccg agctgctcgc gaccgattgt cacagcgtca
     4141 cgcatccgga tgacgtcgcc ggcgatgtcg aactcacccg gcggctggtt gccggcgaag
     4201 tcaacagcac gcaacgcgag aagcgctacc tgcacaagct tggacacgtc gtctgggtgc
     4261 aggtcaccgc ctcgctcgtg cgcggtcgcg atgggacgcc ccgctaccgc atgctccagg
     4321 tgctcgacat ttccaaccgc aaagaggccg aggagcgctt ccgcgccacc ttcaaccagg
     4381 ccgcggtcgg catcgcgcat accgatattg aaggcaggat cctggaagtc aatcacaagc
     4441 tgtgcgaaat attcggtcac ccggaagcag agctgctggg ccgcaccacg cgcgagcttg
     4501 tccatcccga cgatcgcgac cgccaggacg gcttgaggct cgaattgctg tcgggggccc
     4561 gccgttcgtt cagcggcgaa aaacgctgcg ttcgggggga cgggacgacg atctgggtca
     4621 accggaccgt gaccctcgcc cgccaagtaa taagcaacgt gccgtatctc atccaggttt
     4681 tcgaagacat caccgagcgc aagcaaaccg agaaacgtct tgagcgcgcc agccgggcgc
     4741 ggcgggtcat ggtggaatgc aatcacatcc tggtgcacgc caccgacgag acgcagatgc
     4801 tggagagcat gtgccgcatc gtcgtcgaat cgggaggcta caagatggcg tgggtgggac
     4861 ttgcaaccgg tgaccccgtg cgcccggtct acccggcagc ccacgccggc ttcgagaacg
     4921 acgcgccgat gaccggctcc gccgcctggg gcgcggacgg acgctaccgc gggttcatga
     4981 gcgaggtgct ggcgagcggc gagcctcaca tcgcgcgcaa tatcctgaag gatccgacgc
     5041 acgcgggacg ccttgcccgt gccttgcagc gcggcttcca gtcgtcgatc gcgctgccgc
     5101 tgaagggcga cggccgcatc ctcggcgcga tcgccatgta cgcggccgaa cccgacgctt
     5161 ttgacgacga ggaaatagcg ctgctcaagg aactcgccga tgacatcgct ttcggcattg
     5221 tgaacttgcg cacgcgcatc gcccgcagga acgccgaaac agcgctgcag gagagcgaac
     5281 tgcggttccg cgagactttc gagcaggcgg cggtgggcat cacgcgcgtg gacctgaacg
     5341 gggtgctggt ggacgtcaac cagaaattct gcgacatgct cggctacgac aaggacgaac
     5401 tgctcggcaa gtccatcagg gacatcacgc atccggccga ctacggccgg ggcgcgcagt
     5461 accgggcgca gctcgcgcac ggcgccatga agtcggcggc cggcgaaaag cgcttcatcc
     5521 gcaaggacgg caccgccctg tgggcgcggc ggaccatgtc caccgcgtgt gacgataccg
     5581 gcaagccgct ctacgtgatc agcgtggtcg aggacatcag cgagcgcaag gagctggaac
     5641 gccggttcga gctgacgttc aaccatgcgg cggtgggcat gacccaggcc agcctcgatg
     5701 gttacctgct gcaggtcaac cagaaatacg ccgacatgct gggctacacg cgggaggagc
     5761 tgcgcggcat gtcatccgag gcgatcacgc atcccgagga cgtgtctgaa acgctccaga
     5821 cgagaaagga tctgatcgaa ggccggctcg agagcgcgac cagtgaaaag cgcctggtcc
     5881 gcaaggacgg gcgcgtgatc tgggcccgtc gcagcgtatc ggtggctcgc ggcacagccg
     5941 gcgagccgct ctatttcatc gcggtcgtgg aagagatcac cgaccgcaag cagatcgaga
     6001 tcgaacgcgc ccggctcgcg gcaatcgtgg ataactcgaa cgatgccatt atcacccgcg
     6061 atctgggccg cacggtcctc acctggaacc tggccgccga aaaactcttc ggctggactg
     6121 cgcaagaggc gatcggccaa agcattatca tgatggtccc ccccgagcgc cggggcgaga
     6181 acgctcagcg acgggagctg gttcatcgag ggctgcctgt ccccgcgtat gacacagtgc
     6241 ggctcgccaa ggacgggcgg cgcatcgacg tgtcactgtc ccaatccgcc atcaagaacg
     6301 agcacggtga ggtgattgcc gtctcgcttg tcctgcatga catcacggag cgcaagcagg
     6361 ctgaggccga gcgcgcgcga ctcgcagcca tagtggaaag ctcggccgat gcgatcatca
     6421 gccgcaacct cgatggcacg atactcacct ggaacgccgc cgcggaaaaa atgttcgggt
     6481 atgctgcagc agaggtcatt ggccgcgaca tttccttcct tatccccccc gatcggcgcg
     6541 aggaggcgtc ccagaagcgc cgtatcctca atgagggccg cgcggtcccc acgttcgata
     6601 cggtgcgcgt gaccgaggac ggccggggcg ttgacgtgtc tctcacgcta tcgccgatca
     6661 aggacccggc cggtgtgatg atcggcgtct cgctcgtatt ccgcgacatc tccgaacgca
     6721 gggcactgga acggcgctac cgggaaacct tcgatcaggc cgcagtcggc ctgacccggg
     6781 ttgacttgaa cggcgtgctc gtggagtgca atcagaagtt ctgcgacatg ctcggctatt
     6841 cgaggggcga gctgctggga aaaaccatca gggacatcac ccatccggac gactacgggc
     6901 agggcgcgca gtatcgcgcg gacgtggtga gcggcgtcgc caagtcgcgt gcgggcgaga
     6961 agcgtttcct gcgcaaggac ggcaccatga tgtgggcgcg gcggaccatg tccacggcct
     7021 gcgacgagac cggcaagccg cagtacgtga tcagcgtcgt cgaagacatc accgagcgca
     7081 agcaggccga agaacgctac cgcgccactt tcgacaacgc gccggtcggc atcatgcaca
     7141 ccagcaccga tgacgacaaa atcctgcacg cgaactccaa gctgtgcgag atgctcggct
     7201 atacgcatga cgagctggtc tccttgacga ccgatcagat catcgatccg gaccacgtgg
     7261 gcgtggaccg gacgaagtat cgggaaaaga tgctgaacgg cgagctcgat accttctcgt
     7321 cggaacgcct gtaccggcgc aagaacggtt ccggcttctg ggtgaacagg acggtatcgc
     7381 tggcgaggga ctgggcgagc gagccgcttt acttcatccg catcatcgag gacatcagcg
     7441 agcgcaagct cgtggcgagc cgccaggcga tggagcacgc cgtcacccgc gtgctggcgc
     7501 aatccgccag catcgccgaa gcgatgcccg ccgtcctcaa gaccatctgt gagggcttgg
     7561 agtggacttg cggcatctac tggaagtggg atgaaaaggc cgagttgttg cggtgcgcgg
     7621 aagcatggca tcgcgagggc gaggaagtcg cgaatttcgt cacgaccagc aggcaaagcg
     7681 tgaacgaggc gccggcgtgg cacggtaagc ccctcaagat cgaggctggc ggcatcgtgc
     7741 ggcgggtgtg gttcggcggc gtcccggtgt ggtaccccga tgtctcgcgc catccggatt
     7801 tccggcgcgg gccactcgcc gccagggcgg gcctgcattg tgcttttgcg ttccccatcc
     7861 tggcagggga acagcccctg ggcgtcgtcg agttttacag ccgcgatatc cggcagcccg
     7921 atgacgtgct gctgcagatc gtgcgggcca tcggcagcca gattgggcaa ttcatccagc
     7981 gcaaggaagc ggagcaggcg ctgcgccaga gcgaggaacg ctatcgcgac ctgttcgaag
     8041 cgagcccgct gccgatgtgg gtatgggaag acgagacgct tggcttcctg gcggtgaacc
     8101 aagccgccat cgatcattac ggctacagcc gggacgaatt ccggcggatg aacgtgcgcg
     8161 gcatctgggc gtccggagag tgggagcgct acgaggacag tctccgcgat cgcgaccgca
     8221 agcagaatct gagcttgcag cgcaggcacc ggaccaagga cgggcacatc atcgacgtag
     8281 aagtcacggc gcggcggttc gtgcacggcg ggcgtcccgt ctggttgaca ttgctcaacg
     8341 acgtcaccga gcgcaagcgg gcggaagcgg ccctgcgcga gagcgaggag cacctccgcg
     8401 ccatgttcga ccaggcgacg gtcggcatca cggtctccgg tctcgacctg cagtacctgc
     8461 aggtcaacga caagtactgc gatatcgtcg gctattcccg cgacgaactg ctgcggatgt
     8521 cggtcaggga cgtgaatttc tccgagtaca tggaagacgc gacaaagtac cgggagaagc
     8581 tgtttcgcgg ggagatcgcg agcacggtcc gcgagaaacc gctccggcgc aaggacggca
     8641 cggcggtatg ggtttcgatg gtgacttcga tcgtccgcgg caacgaaggc aagccccaac
     8701 agttcatctc ggtgatccag gacatctccg agcgtaagcg ggcggaggtc gccctgcgcc
     8761 agagcgagga gcagtttcag cagctcgcca acaatatccc ccaggtgttc tggatcgcgg
     8821 acgccggaca gaagcagacg ctgtacgtca gtccggccgc cgagaccctg atcgggcgcc
     8881 cgctcggtga aatccaggcc tcgccgcgct cgctggtgcg cgcgttgcac aaggaagatc
     8941 gtccgcgcgt ccgtgccgcg cggaaggacg ccgcggcggg cggttatgac cagacctatc
     9001 gcatcgtcag gccggacggt tccatccgct gggtgcgcga ccgggcgttc ccggtgcacg
     9061 acgattccgg ggcggtgcac cgcatcgccg gcatcgcgga ggacatcacg gaccgcaaga
     9121 tggcagaaga gcgcctcctg cacctggcgc actacgacgt gttgacgagc ctgccgaacc
     9181 gcgtgctgtt ctacgaccgg ctgcggcaag cgctcgctca ggccaagcgc aaccagtgga
     9241 cggtgggcgt ca
//
LOCUS       DBNGWZ010000019         1574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2268443, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000019 DBNGWZ010000000
VERSION     DBNGWZ010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2268443"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="AC16CX_00510"
     CDS             <1..1254
                     /locus_tag="AC16CX_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3011023.1"
                     /translation="PPDLPISRMVSSAHHQRLTPVQLTLLMCGAEIFCMTGFAAYTTL
                     LPALQREWGLSNSEAGLISGIYYAGYVAATPVLTSLTDRVDARRVYMLACALSLAGAA
                     GFALLAGGLWGALIFQFLIGAGLGGSYMPGLKMLADQLDGPVQSRAVAFYTASFGVGS
                     TLSIFVCGLIGGAWGWQWAFAYGALGPMLAASLVSLVMPRGRTRAPGKPAPALLDFRP
                     VLRNRKTLPYIAGYSAHCYELFGQRSWMVAFLVFTASLQPANAPMLVSAATLAATINL
                     MGPVMSVSGNELAIRFGRTRVIFTFMTASGLVACVLGYTAALPWMLVFLIMCVHYGLM
                     LGDSAALTSGAVASAHPDERGATMAIYSFAGFSSAFLAPLVFGVVLDLAGGNRSLLAW
                     GLAFASIGIFGVLSPLARWLYLRRA"
     gene            1378..>1574
                     /locus_tag="AC16CX_00515"
     CDS             1378..>1574
                     /locus_tag="AC16CX_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011024.1"
                     /translation="MYAGIFTVTEASAVGINVFVVRGVAEDVPMGKIFAGIMPFWAAM
                     AVCVALLIAFPQIALVLPDSM"
BASE COUNT          183 a          534 c          524 g          333 t
ORIGIN      
        1 cctcccgacc tcccgatctc ccggatggtg agttccgctc accatcagcg gctgacgcca
       61 gtccagctca cgctgctcat gtgcggcgcg gaaattttct gcatgaccgg gttcgcggcg
      121 tacaccacgc tgctgccggc gctgcagagg gagtgggggc tgtccaattc cgaggcgggg
      181 ctgatcagcg ggatctatta cgccggctac gtcgccgcga cccccgtcct caccagcctg
      241 accgaccggg tggacgcgcg cagggtctat atgcttgcct gcgccttgtc gctcgccggc
      301 gccgcggggt tcgcgctgtt ggctggcggg ctctggggcg cgctcatctt tcagttcctg
      361 atcggggcgg ggctcggcgg cagctacatg ccggggctga aaatgctcgc cgaccagctc
      421 gatgggccgg tgcagtcacg cgcggtcgcc ttctatacgg cgagcttcgg cgtgggctcc
      481 acattgtcca tcttcgtctg cggtttgatc ggcggcgcct ggggctggca atgggcgttc
      541 gcttacggcg cgctgggacc gatgctcgct gcgtcgttgg tcagcctcgt gatgccccgc
      601 gggcgcacgc gcgcgccggg caagccggcg ccggcgttgc tcgatttccg tccggtgttg
      661 cggaaccgga aaacgctgcc ctacatcgcc ggttattccg cgcactgcta cgagctgttc
      721 ggccagcgct cgtggatggt ggcgttcctg gtcttcaccg cgtcgctgca accggcgaat
      781 gcgccgatgc tggtgagcgc ggcgacgctc gccgcgacga tcaatctcat ggggccggtg
      841 atgagcgtct cgggaaacga gcttgcgata cgcttcggcc gcacgcgcgt gatcttcacc
      901 ttcatgaccg cctcgggcct cgtcgcctgc gtgctgggct acaccgccgc gctgccgtgg
      961 atgctggtgt tcctcatcat gtgcgtgcac tacggcctga tgctgggcga ttccgccgcg
     1021 ctcacctccg gcgcggtcgc ttccgcccat cccgacgagc gcggcgcgac gatggcgatc
     1081 tattccttcg cgggattcag ctcggcgttc ctcgccccgc tcgtgttcgg tgtcgtgctc
     1141 gatctcgcgg gcggcaaccg cagcctgctc gcatgggggc tcgccttcgc gagcatcggc
     1201 atcttcggcg tgctgtcgcc gctcgcgcgc tggctctacc ttcgccgcgc ctgaacgttc
     1261 gtctcccggc tggttgcgct gcagcaacgg gctgtttccc attgcgtaaa acggccgcgg
     1321 cgtcggcacg tccctctttg ctttccccgt gccaggggtt acattttccc cggcggcatg
     1381 tatgccggga tcttcacggt taccgaggcg tccgcggtcg gcatcaacgt cttcgtggtg
     1441 aggggcgtgg ccgaggacgt gccgatgggt aaaatcttcg ccggcatcat gccgttctgg
     1501 gcggcgatgg cggtctgcgt cgcgttgctc atcgccttcc cgcagatcgc actcgttctg
     1561 cccgactcca tgat
//
LOCUS       DBNGWZ010000020         4070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_631333, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000020 DBNGWZ010000000
VERSION     DBNGWZ010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_631333"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1645)
                     /gene="istA"
                     /locus_tag="AC16CX_00520"
     CDS             complement(149..1645)
                     /gene="istA"
                     /locus_tag="AC16CX_00520"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR3011025.1"
                     /translation="MEAETGVRRETVSRYDEARRTNAAKTFPGSDGSPAVDCGDLAAG
                     GGSNAATTFAGSEPNPAKTFLGPAARPRFAAAIYRTAITEKLDAGLSVQRIWQDLVEE
                     YGYGASYESVKRFVRTHAPTRRAVGVFHCAPGAEAQVDFFRGAPTLDSATGEWRRPWV
                     FRMTLGHSRHGYEEGVWDQKLETFVRLHERAFRDLGGVPRVIRHDNLKAAVVRACFFD
                     PDSHEVYLAFAAHWGFTPLPTQPRRPQENGKQERSGGYVKDNALKGRRFDSLEAHNAF
                     LRQWNRTIARLRIHGTTRRQVWTHFAEVEQRALQPLAGEAFALFTTGERSVHTDGHVE
                     VAGAFYPVPLALLGQRVRVRWDAHLVRVFQGDALVMVHARVAPGVFAPRAGEAQASTR
                     QQAYVERLVGQCERLGPALKQWAEAALAVRGVRAIRLIQGVLGLTRRHPRERVLAAVT
                     EAHAHQHFRYQTIRQLVERTPAGPAPTLLSEDPAIRPMTQYTLDDFLR"
     gene            2062..3231
                     /locus_tag="AC16CX_00525"
     CDS             2062..3231
                     /locus_tag="AC16CX_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR3011026.1"
                     /translation="MYLGKTLFAQVMDFLPWKTFHRIVDRHGGNHRIRTLSCAEQFRV
                     MAFAQLTYRESLRDIEACLSAQAGKLYHMGVREPVRRSTLADANESRDWRIYYEFAQR
                     LIVKARTLYANEDFGVELANTVYALDATTIDLCLSMFPWAPFRSTKAAVKLHTLLDLR
                     GSIPTFVHISDGKLHDVNVLDLLIIEAGAFYIMDRGYLDFERLYALDQAGGFFVTRAK
                     RNLDARRLYSALVDRSTGLISDQTIALNGFYAAKHYPGHLRRIRYRDPETGKTLVFLT
                     NQFVVPALTICALYKSRWSVELFFKWIKQNLRIKRFYGTSENAVRTQIWIAISVYVLV
                     AIVKKQLNLEVSLHTLLQILSVTLFEKLPLQQAVADIAPVTNDEVMHNQLKLFEL"
BASE COUNT          767 a         1266 c         1255 g          782 t
ORIGIN      
        1 gcgcgccgcg cgagctcgtc ctcgacgagc agttcgagaa actccagatg cggcagcggt
       61 gcagcttcgg cttgcgcgag gcgcccgggt agcgcctcga ccatgccaga gaggcgcaga
      121 tgacgcagtc gggtcgtgag ctgtggattc atcgcaggaa gtcgtccaag gtgtattgag
      181 tcatcgggcg aatcgcagga tcctcgctga ggagcgtggg cgccggcccc gccggcgtgc
      241 gctcgacgag ttggcgaatg gtctgatacc gaaagtgctg atgggcgtgg gcctcggtga
      301 cggcggccag cacgcgctct cgcggatgcc gccgggtcag cccaaggacg ccttgaatga
      361 gccgaatggc gcgcacgcca cgcaccgcga gggcggcctc ggcccattgc ttgagcgccg
      421 ggccgagccg ctcgcattgg ccgacgagtc gctccacgta ggcctgctga cgcgtcgagg
      481 cttgcgcctc gccggcacgc ggggcaaaca cgccgggggc cacgcgcgcg tgcaccatga
      541 caagcgcatc gccctggaag acgcgcacca ggtgtgcatc ccatcgcacg cgcacgcgct
      601 ggccgagaag cgccagcggc accggataga aggccccggc cacttcgacg tggccatcgg
      661 tgtggacgct ccgctccccg gtggtaaaca acgcgaaggc ctcgccggcc agtggttgga
      721 gcgcccgttg ctcgacctcc gcgaagtgcg tccagacctg gcgccgcgtg gtgccgtgga
      781 tgcgtagccg cgcgatcgtg cgattccact gccgcagaaa cgcattgtgc gcctcgaggc
      841 tgtcgaagcg ccggcctttg agcgcgttgt ctttgacgta gcccccgctg cgctcctgct
      901 taccgttttc ctgcgggcgg cgcggctgcg tgggcagggg cgtgaacccc cagtgcgccg
      961 cgaacgcgag atagacctcg tggctgtcgg gatcgaagaa gcacgcgcgc accaccgccg
     1021 ccttgagatt gtcgtggcga atgaccctgg gcacgccccc gaggtcgcgg aacgcgcgtt
     1081 cgtgcaggcg cacaaacgtc tcgagtttct gatcccacac cccttcctcg tagccatgcc
     1141 gcgagtggcc cagcgtcatg cgaaacaccc acggacgtcg ccactcgccc gttgcgctgt
     1201 ccagcgtggg cgcgccgcgg aagaaatcta cctgcgcttc ggcaccaggg gcgcagtgaa
     1261 acacgcccac ggcccggcgt gtgggtgcat gcgtgcgcac gaaccgtttc accgattcgt
     1321 agctcgcccc gtacccgtac tcctcgacca ggtcctgcca gatgcgctgc acactcagac
     1381 ccgcgtcgag tttctccgtg atggccgtgc gatagatcgc ggcggcgaac cgcgggcgcg
     1441 ccgcggggcc gagaaacgtt ttggccgggt ttggctccga gccggcgaac gttgtggccg
     1501 catttgaacc tccacccgcc gcaagatcgc cgcaatccac cgctggcgac ccatcagagc
     1561 cggggaacgt tttggccgca tttgtccggc gcgcctcgtc atagcggctc accgtttcgc
     1621 ggcgcacccc ggtctcggcc tcgatgcggc gctagctcca gccgagctcg agtagggcta
     1681 gaacttgcga tttcttcggc atcttcaggt agttgggcat cgacatcatc aagggggtga
     1741 aaccctcgag gatgcctgaa taccctacct gcagtggccg gttttcagac gttcggtcgt
     1801 ggccgcattt cagcgttcgc taacagcata gaacacgcca tagatgatga acaggacgat
     1861 gacctgccat tgcgtggtcg caaatgcgaa gccggggctc atcaatagat agatcaggtc
     1921 agaagtgtcc ggttaacttt gggggtatcg gcttgaaagc caatactgac ttgggtttcc
     1981 aggcgaaggg gggtgtctcc gacttgaatc ggaaatcgcc acttgcgcta gcgttcgggt
     2041 ttttatcaac ccggagctgg catgtatctc ggcaaaacgc tgttcgcaca ggttatggac
     2101 ttcctgccgt ggaagacctt ccatcgcatc gtggatcgcc acggcggaaa tcaccggata
     2161 cggaccctca gttgtgccga gcagtttcgc gtcatggcct tcgcgcagct cacttaccgg
     2221 gagagcctgc gcgatatcga ggcgtgtctt tcggcgcaag cggggaagct ctaccacatg
     2281 ggtgtgcgcg aaccggttcg acgctcgacg ttggcggatg ccaacgaatc gcgcgattgg
     2341 cgcatctact acgagtttgc ccaacgactg atcgtgaaag cacgaacgct ctacgccaac
     2401 gaggacttcg gcgtggaact ggccaatacg gtttatgcgc tcgacgccac gaccatagat
     2461 ctgtgtctgt cgatgttccc ctgggcgccg tttcgctcca ccaaagcggc agtgaagctg
     2521 cacacgctgc tcgatttgcg cggctcgatt cccaccttcg ttcacatctc cgatggcaag
     2581 ctgcacgatg tcaacgtcct ggatttgctc atcatcgagg cgggcgcctt ctacatcatg
     2641 gatcggggct acctggactt cgagcggctc tacgctctgg accaggcggg cggcttcttc
     2701 gtcacacgcg ccaagcgaaa cctcgatgca cgacgcctgt attccgcgct ggtggatcgc
     2761 agcaccggac tgatcagcga ccagaccatc gcgctcaacg gcttctacgc cgccaagcac
     2821 taccccggcc atctgcgccg catccgctat cgcgaccccg aaacgggcaa gaccctggtg
     2881 ttcctgacca atcagttcgt tgtgccggca ctgacgatct gcgcgctgta caagtcccgc
     2941 tggtctgtgg agctgttttt caagtggatc aagcagaatc tgagaatcaa gcgcttctac
     3001 ggcacctcgg aaaacgccgt cagaacgcaa atctggatcg ccatcagcgt ctatgtgctg
     3061 gtggcgatcg tcaagaagca actcaatctg gaggtatcgt tgcatacgtt attacagatc
     3121 ttgtcagtga cgctcttcga gaaactgccg ctgcaacagg cagttgctga tattgccccg
     3181 gtcactaatg acgaagtcat gcacaatcaa ctgaaactat tcgagcttta accggacact
     3241 tctggttgtc aatatatatc aagccgtagg cgtattgccg acgtgtctcc cggccagcgt
     3301 gacaacagcg tcttgggcgt tctatccgca tgattagcgg gatagtgaag tgcatgttga
     3361 tgtccgcccg gcccgatgcc aaggccgatc tttgagcgtg acgaaaacgt gacgtagccc
     3421 gcgggtattg gcgtgtgttg gcgggtgagg gcgggccaaa tcgctagtac acaagttgag
     3481 aataacgaca cataatcctt tggtcctggg ttcgagtccc agcgggccca tcattgaaaa
     3541 caagtcctta cgaacctgcc tcagtggcca agcccctagc tacgcgccaa agcgtcgaag
     3601 aggattaaag ggcgagtgca gccgttttgc gcgctcagga cgagcagtcg ttagcgcgag
     3661 atgtcgaacg ctacatccgc cagatgtctc cggtggccac ggacaaagag ttgcttgcca
     3721 caggcctgat ggagcaattg gcgacgcagc gatcacgcat cgttgttgcc caaccgcagc
     3781 attcggtcgg cgggcgcgct cggtaagggc ttcgcgtgaa gctgcctcgc caggctgaac
     3841 cacgatcgag cctgccgttg cggcgaaccc cttatcccgt ccttctacgc ggcacgggtc
     3901 ttctcatcac catcgtcagc cggaggatcg tttcgcgctg ctcggggtcc agggcgcggt
     3961 aagtatccag ccagacgcgc tagtcctcgg tgaaccgctc cacctcaaag cgcaacaacg
     4021 cacgatggcg aaggcgttat agccggtggt ccttcagcga tgcgttgagt
//
LOCUS       DBNGWZ010000021         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1261422, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000021 DBNGWZ010000000
VERSION     DBNGWZ010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1261422"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16CX_00530"
     CDS             <1..353
                     /locus_tag="AC16CX_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR3011027.1"
                     /translation="GVPLNRVLRRIRETREQRYSLFRGFFRGVTDETGGEDEAAQPRL
                     RSVIIAPGASAIGRTLGETELSRLSVEVTAVRRHNIRGVAPEPTTRVEEGDVLVLLGA
                     EEDLAAAEIKLLQG"
     gene            complement(492..1187)
                     /locus_tag="AC16CX_00535"
     CDS             complement(492..1187)
                     /locus_tag="AC16CX_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3011028.1"
                     /translation="MRKQQAGFTLIEIAIVLVIIGLLLGGILKGQELITSARVRNLIS
                     QQDGVKAAYFGFLDRFRALPGDYNQAVANIAGVGAGSDGNNNGQITANGVSGATTDEH
                     IAAWDHLSKAGFINGSYTYALAPETTATAPTNPYARYLQLIYDNNYDPAASATRHNLK
                     TGNQIPSDILAEVDRKIDDGVATSGTFRFSAYGGNTGAAAIAGSGTCYAAVAPNNWLS
                     ATPATNCGGTSLF"
     gene            complement(1422..2195)
                     /locus_tag="AC16CX_00540"
     CDS             complement(1422..2195)
                     /locus_tag="AC16CX_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011029.1"
                     /translation="MRGSGFGARDSTSAAERAIGTVPRWVLVLLAVAFCLQVGWQALQ
                     PKPSARAEALGAPAPPAALRALALGEPIALAQLLTLYLQAFDNQPGVSIPFLDLDYSR
                     VTAWLQTVLALDPIGQYPLLMASQIYGQVPDKAKQRRMCEFVRQQFDFDPNRRWRWLA
                     HCAIMAKHRLGDPRLALSYAEAITRDARLASNWARQMRIFILEDLGEQEAAKVLLGGL
                     LASGEVTDPKEIHFLTERLEKLKAAEKSSSLSGNRRPEP"
     gene            complement(2195..>2877)
                     /locus_tag="AC16CX_00545"
     CDS             complement(2195..>2877)
                     /locus_tag="AC16CX_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011030.1"
                     /translation="TLIAASFFIETISVTEGARFQAGFYAAGMRLACVFIAGLYVLVS
                     VTREFNDKGLDVLLALDLPRSHYVLGKLAGFLAIAALIAVTASLPLMWLAAPQAALQW
                     AVSLAVELAVIVALALFCISAFGQLTPAASFVLAFYLLARSLTAMRLMGAYPLTGADT
                     LSHQVIHFLVETLALVLPSLDAWTQTAWLVNQQASWFSILELTGQSALYVTLLAAATM
                     FDFYRKNF"
BASE COUNT          607 a          851 c          896 g          523 t
ORIGIN      
        1 tgggggtgcc gctcaatcgc gtgctgcggc gcatccgtga gacccgcgag cagcgctaca
       61 gcttgttccg aggtttcttc cgcggcgtca cggacgagac gggcggcgaa gacgaggcgg
      121 cgcagccgcg gctgcgctcc gtgatcatcg cgcccggagc gtcggcgatc ggccggacgc
      181 tcggtgaaac cgagctttcg cgcctgagcg tggaagtgac ggcggtgcgg cggcacaaca
      241 tccgcggcgt cgcgcccgag cccaccacgc gagttgagga aggcgacgta ctggtgctcc
      301 tcggcgccga agaggatctg gcggcggcgg agatcaaatt gctacagggc tgactccggt
      361 taccagttcc aagttacaag ttccaaggtc caagttcgag gttgcaacga tgcggtaaca
      421 ctggaaataa aacgcccgcc gaagcgggcg ttaatccttg aaccttgata cttggagctt
      481 ggaaccgggg cttagaacag gctggtgccg ccgcagttgg tagcgggggt ggctgacagc
      541 caattgttag gcgccaccgc agcatagcag gtgccggaac cggcgatcgc cgcagcgcca
      601 gtgttgccgc catatgccga aaaccggaaa gtaccactgg ttgctacacc atcgtcaatc
      661 ttccggtcga cctcagccag aatatcggaa ggtatctggt tgcccgtttt cagattgtgc
      721 cgagtcgcgg aagccgcagg atcgtagtta ttgtcgtaaa tcaattgcag gtaacgcgcg
      781 tatggattgg tcggtgctgt tgccgtcgtt tccggagcga gggcataggt atagctgcca
      841 ttgataaacc cagctttcga caaatggtcc cacgccgcga tgtgctcatc ggtcgtcgcg
      901 cccgatacgc cgttggctgt aatctgaccg ttattattac cgtcagaacc tgccccaacg
      961 cctgcaatat tggctacggc ctggttgtaa tcgccgggga gcgcgcggaa gcggtcgagg
     1021 aaaccgaagt aggcggcctt gacgccatct tgctgcgaga tcaggttgcg cacgcgcgcg
     1081 ctggtgatca gttcctggcc cttgagtatg ccgcccagca gcaggccgat gatcacgagc
     1141 acgatcgcga tctcgatcag ggtgaagccg gcttgttgtt ttcgcatgat ctatctcctc
     1201 caattgcccc ggtggattca gtttccgcaa gatctatgcc aataaaaaag ggttgcgcat
     1261 gaaccaagcg caacccttct ttatctgtga aatttttcac accgacccga tttctgatct
     1321 tatgctattg taatacaaca tattatcaaa aatcgtgcac cgatggatag agctgcgcga
     1381 tttttgatta gtggttgaaa aataaggaaa tgttgtaata gtcacggttc tggccgtcgg
     1441 tttcccgaca gcgacgacga tttttcagca gccttcaatt tttcaagtct ctcggtcaga
     1501 aaatgaatct ccttagggtc ggttacttcc ccgctcgcga gcaggccacc gagcagcact
     1561 ttggctgctt cctgttcacc cagatcctcg agtatgaaga tgcgcatctg gcgcgcccag
     1621 ttggaggcaa gccgggcgtc gcgcgtgatg gcctcggcgt aggaaagcgc gagacgcggg
     1681 tcgccgagac gatgcttggc catgatcgcg cagtgggcga gccaccgcca gcgccggttg
     1741 ggatcaaaat cgaactgctg gcgcacgaat tcgcacatcc ggcgttgttt ggccttgtcc
     1801 ggcacctggc cgtagatctg tgacgccatc agcagggggt actgaccgat cggatcgagc
     1861 gcgaggaccg tctggagcca cgccgtgacc cgcgaataat ccagatcgag aaacgggatg
     1921 ctcacgcccg gctggttgtc gaacgcctgc aggtaaagcg tgagcagctg ggcaagggca
     1981 atcggctctc ccagcgccag cgcgcgcaaa gcggccggcg gcgccggcgc ccccagcgcc
     2041 tccgcccggg cgctcggctt cggctgcagc gcctgccatc ccacttgcag acagaacgcg
     2101 acagcgagca ggacgagcac ccagcgcggc accgtaccga tcgcgcgctc tgccgcgctc
     2161 gtcgaatccc gagccccgaa tcctgagccc cgcatcagaa gttcttccgg taaaagtcga
     2221 acattgtcgc cgcggcgagc agcgtcacat acaacgcgct ttgccccgtc agctcgagaa
     2281 tggaaaacca cgaagcctgc tggttcacga gccaggcggt ctgggtccag gcatcaagtg
     2341 aaggcaggac cagcgcaagc gtctcgacca gaaaatggat cacctgatgt gagagcgtat
     2401 cggcgccggt caggggataa gcgcccatca gtcgcatcgc cgtcagcgag cgggcgagca
     2461 ggtaaaacgc cagaacgaag ctcgcagccg gggtcagctg gccgaacgcg ctgatgcaga
     2521 acagggcgag cgcgacgatg acggcaagct cgacggcgag ggaaaccgcc cattgcagcg
     2581 ccgcctgcgg cgcggccagc cacatgagcg gcagggaagc agtgaccgcg atcagcgccg
     2641 cgatggccag aaagcctgcg agtttgccca gaacataatg cgaccgcggc aggtcgagag
     2701 cgagcagcac atcgaggccc ttgtcgttga actcgcgcgt cacgctcacc agcacataga
     2761 gccccgcgat gaaaacgcag gcgagccgca tgccggcggc gtaaaaaccc gcctgaaacc
     2821 gcgcgccctc ggtgaccgag atggtttcga tgaaaaagct tgccgcgatc agcgtga
//
LOCUS       DBNGWZ010000022        11397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2395186, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000022 DBNGWZ010000000
VERSION     DBNGWZ010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2395186"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="AC16CX_00550"
     CDS             <1..696
                     /locus_tag="AC16CX_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3011031.1"
                     /translation="VLFREAVGQRLWLATLLMLAGGFVLAYDPGAPLAFSLQSLAIVS
                     ACFGWAVDNNLTRKVAGADPVAIAMTKGIAAGSFNLALGLALGGKLPAFGPAAGALAL
                     GFIGYGVSLVLFIYALRHLGAARTAAHFSTAPFIGAALAVPLLGEPVTTGLLAAAALM
                     AIAIWLVITESHLHEHEHGHQVHTHRHVHDEHHQHAHHGDEGPEPHVHEHTHEPLRHA
                     HPHVPDLHHRHEH"
     gene            complement(719..1693)
                     /gene="senB"
                     /locus_tag="AC16CX_00555"
     CDS             complement(719..1693)
                     /gene="senB"
                     /locus_tag="AC16CX_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HIR3011032.1"
                     /translation="MKIAITVPPSAVPRSGNRHTAARWARFLRELGHRVRIVTEWRSG
                     NDDLLLALHARKSFASIRRFSDLRPDAPLVVALTGTDLYRDIGKYPEARQSLGLAARL
                     VVLQEEGLRELSPFLRRKTSVVYQSSDVTLRHAPELSRFRIAVIGHLRDEKDPFRAVM
                     TLRRIDRSAPVEVLQIGAALDPEMRARCKAWMRCESRYRWLGSLPHRRAMRWLASSHV
                     LVVSSVMEGGANVICEAARIGVPVLASRVPGNIGMLGRDYPGYFPLFDDKALARLIER
                     SRTDAGFYTRLRRTLSRRRALFAPAAERAALKRLLQALPGNTRRAERG"
     gene            1760..3034
                     /gene="senA"
                     /locus_tag="AC16CX_00560"
     CDS             1760..3034
                     /gene="senA"
                     /locus_tag="AC16CX_00560"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HIR3011033.1"
                     /translation="MEPGSLAGELRASRAHLTRITADLDGDQLLGPRLAIVNPPLWEL
                     GHVAWFQEYWCLRRSTLGKTVDSVVPGADALYDSAKVAHDTRWDLPLPGLKSTLSYQA
                     EILERVIRRLGKEPENRELQYFVQLAIFHEDMHAEAFHYTRQTLGYEDPFHNNEIAPS
                     AHERVHGDVSLPGGTFLLGASPGRDFVFDNEKWAHEVRLRPFRISRTPVTNAEYVKFV
                     ESGGYSRREWWSDDGWAWKSREGLHAPKYWAKRDGAWAQRRFDRVIALRPDEPVIHVS
                     WHEAQAYCRFARRRLPTEAEWEYAAAWDPHAGGKKHYSWGNQLPDAARANLESAGIAG
                     VDAYPTGDTASGCRQLIGNVWEWTESTFNPYPGFIIDPYQEYSQPWFGTHKVLRGGSF
                     ATTRRLIRNTWRNFYTPDRYDVFAGFRTCAAE"
     gene            3031..4644
                     /locus_tag="AC16CX_00565"
     CDS             3031..4644
                     /locus_tag="AC16CX_00565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR3011034.1"
                     /translation="MSPRGRRRFILGAAGIAGAAALAAGFLRSHRPRLDELTARFEPP
                     LPPDPAFPNPLRLPGAEGMFGVAEAAGALTIIAKGIEYPILPGKPARMLAYEMEHHGR
                     TLLNPVLRARSGAQLRIKFWNGLEETSIIHWHGLKVDSNNDGHPHYAVPGGATYDYQF
                     TVANRAATYWYHPHPHHLTGKQVYHGLAGFFIVEDDEELALQKSLDLRFGETDIPLVI
                     QDRRLDENGRLIFTPTPDERFHGQYGDQVLVNWTPRPHFHAATRFHRFRILNGSNARL
                     YRLAFRHGERLLDYAVIGTDGGLLDRPRSVREAFLAPGERIDVLLDLRAAARGDAVTL
                     VNLPFDAMHTETKRAQGSHEEHAAHAATPDGAEAELLRIRVQSKTRYDRTLPQTLSRI
                     EPVAESPYSPRLIVLDQAKMAWRINAATYDVAATPITVRRDSVEAWDIRNVPPTMPHP
                     MHIHGFQFRVVSREGSPEQQRRLAVQKSGLAASDLGWKDTVLVWPGETVRIVTDFSHP
                     FLGDQVYMVHCHNLEHEDSGMMLNLKVTA"
     gene            5009..5299
                     /locus_tag="AC16CX_00570"
     CDS             5009..5299
                     /locus_tag="AC16CX_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR3011035.1"
                     /translation="MKPQNVINPEAYRARFGIPNAFGSCHTAVIEGYAIEGHVPAREI
                     QRLLAERPKARGLAVPGMAAGSPGMEGSRSVPYDVLLVLPDGSYRVYARYGR"
     gene            5376..6038
                     /locus_tag="AC16CX_00575"
     CDS             5376..6038
                     /locus_tag="AC16CX_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3011036.1"
                     /translation="MKPPRLAVLVLALLFAAADAPSAEPVAPDALTTTMRPVSPGRDH
                     VFGNPRAEISLIEYSDFECPFCKRFHETAKQVVQAYGGRVNWVYRHFPLPFHNPAAQR
                     EAEAAECAAELGGNNAFWRYADLIFGRTPLNGQGILEKELHEMAASIGLDRARFAACL
                     RSGRKALRVDDDFIEGQAIGIRGTPGNVLRNNRTGAIMLRQGAVPLEQIKGVIDQLSK
                     QK"
     gene            6123..6605
                     /locus_tag="AC16CX_00580"
     CDS             6123..6605
                     /locus_tag="AC16CX_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011037.1"
                     /translation="MSSLRNRVGVGLLTASFAFLVGAIVYALWSGEKPLADPDNPRQV
                     ARGKPVYDRHCVSCHGVRLEGQHNWQERLPSGRYPAPPHDFSGHTWHHPDSMLFGMTK
                     YGLVPGKYAPPKYESDMPAFGGALSDEEIWAVLAYIKSSWPSDILKAQHEMTRERDRR
                     "
     gene            7333..7836
                     /locus_tag="AC16CX_00585"
     CDS             7333..7836
                     /locus_tag="AC16CX_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011038.1"
                     /translation="MKRSILCAAVAAIALSAPIALAQDTKPAAGKPAAMEMDKQMAQM
                     QENMKKMQQQMDKIRQAKDPKERQKLLQEHMQSMQEGMNMMRGMGGPMMMGGQGGMGP
                     GMMGGGQMGGGQKGGMGPGMMGGGPMTGMDPKQQQDFMQRRMDMMQMMMEHMMQHQQM
                     MQPAPAK"
     gene            7852..8319
                     /locus_tag="AC16CX_00590"
     CDS             7852..8319
                     /locus_tag="AC16CX_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011039.1"
                     /translation="MNTYAIPAARPTGFGVLAFGLLLAAYFAVLTLVSDWKFTVDQFS
                     EFWFYVLPLAAGFGLQVALYVRLRQLLHRSKDAGTVMAASGTTSTAAMVSCCAHYLVN
                     VAPVLGATGLVTFATQYQVEFFWVGLAFNVAGIAYIGNKLWNATKEHAQCAHA"
     gene            8304..8813
                     /locus_tag="AC16CX_00595"
     CDS             8304..8813
                     /locus_tag="AC16CX_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011040.1"
                     /translation="MRTCLTVFFSGILMTLLVPFQLISPGLGGLPANNDTAEQNQFLF
                     PQIVGTALAAGAFAAELGIQRSSANGVTVAATPQNLAASAKSWDFKVALDTHSGELKD
                     DLVKSAVLVVDGGASYTPTAWQGDPPGGHHREGTLSFNAIAPQPKAIELQIQRPGEPK
                     PRSFRWQLK"
     gene            8827..9216
                     /locus_tag="AC16CX_00600"
     CDS             8827..9216
                     /locus_tag="AC16CX_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3011041.1"
                     /translation="MYGFSTRLKVPFDSAVEKVTAALKTEGFGVLTDIDVKATMKTKL
                     NIERRPYRILGACNPPLAHRAIEADPDIGLLLPCNVVVREEDDGRIWVGFMDPEAVLK
                     LVDKPEVHTLGKEVRARLQRVCNSLGS"
     gene            9233..9562
                     /locus_tag="AC16CX_00605"
     CDS             9233..9562
                     /locus_tag="AC16CX_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3011042.1"
                     /translation="MDTETLKSLGSLLLWGLLFFLMMRFGCGAHTMGGHGHGGHGSHG
                     GSGGEKDPVCGMAVDPEKAAGASVHAGKTYYFCSTSCRDEFEKEPAKYAAAPARDEHQ
                     HGGHCHG"
     gene            9555..9803
                     /locus_tag="AC16CX_00610"
     CDS             9555..9803
                     /locus_tag="AC16CX_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR3011043.1"
                     /translation="MGRLYWRDVALAMGSFLAITYVFCVAYDLAFGQRMYEVWLKLLP
                     GFTWISWPSFFLGLVESFLYGIYFGLVFVPLYNFFHGK"
     gene            9869..10150
                     /locus_tag="AC16CX_00615"
     CDS             9869..10150
                     /locus_tag="AC16CX_00615"
                     /inference="COORDINATES: protein motif:HMM:NF039498.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR3011044.1"
                     /translation="MDTTKPWVTGAALAVTAGVVYLVCAVAVALFPDGTLAFFSTWAH
                     GVDLTLVRRPAAKPLTAEEWIYGFVSIVVASYLAGALFGWARNLFSAWK"
     gene            10294..11067
                     /locus_tag="AC16CX_00620"
     CDS             10294..11067
                     /locus_tag="AC16CX_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3011045.1"
                     /translation="MVELPHTSSAEHVCRHCGAPISGVPYVREHDEYGCESCFLASRK
                     RRTLPGGVDETQFALSEALVCALDAREHETGQHSKRVACHTLVLARRFTADSQQLRQT
                     YWGALLHDIGKIGIPDSILLKQDGLTATEWKIMRTHPEIGHRILSGAPFMAEAAEIVL
                     AHEERFDGTGYPRGLAGAAIPLWARLFAVIDTLDAMTSDRPYRRGLPFEAARAEIARQ
                     SGTQFDPQAVEVFMAEDAALREMVALKCGATAIPDAVPV"
     gene            11099..>11397
                     /locus_tag="AC16CX_00625"
     CDS             11099..>11397
                     /locus_tag="AC16CX_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3011046.1"
                     /translation="MSYVETPADFPYPWYLRLMFRRQRRRYGRELEPVRLWARMPAVF
                     LAMSAMYRALDRKSSRIEPGLRSLIQVRVSQINGCEFCVDLNSFLGLGRGIAEDKL"
BASE COUNT         2142 a         3664 c         3559 g         2032 t
ORIGIN      
        1 gttctctttc gtgaagccgt cggccagcgc ctgtggctcg cgacactcct catgctggcg
       61 ggcggtttcg tgctcgccta tgaccctggc gcgccgctcg cgttctcgct gcagtcgctt
      121 gcaatcgtca gcgcctgctt cgggtgggcg gtcgacaaca acctgacccg caaagtcgca
      181 ggcgccgatc ccgtcgccat cgccatgacc aaaggaatcg ccgcggggag cttcaacctc
      241 gcgcttgggc tcgcgctggg cgggaaactc cccgctttcg gaccggctgc aggtgcgctc
      301 gcgttgggtt ttatcggtta cggcgtgagt ctggttcttt tcatctacgc gttgcggcat
      361 ctcggagccg cgcggaccgc tgcgcatttc agcaccgccc ctttcatcgg ggcggcgctc
      421 gccgtgccgc tgctcggcga gccggtcacg accggactcc tcgccgcggc tgcgctgatg
      481 gcgatcgcaa tctggctagt aattacggaa tcgcatctac acgaacacga gcacgggcat
      541 caggtgcaca ctcaccggca cgttcacgac gagcaccatc agcacgcgca ccatggcgac
      601 gaggggcccg agcctcacgt ccacgagcat acgcacgagc cgctgcggca cgcgcatccc
      661 cacgtgcccg acctgcatca ccgccacgaa cattaattga acggctcgtc gcgggtatct
      721 atcctcgctc agctcgccgc gtattacccg gcaacgcctg caacaggcgc ttcagtgccg
      781 cccgttctgc ggctggggca aatagcgcgc gcctgcgcga gagcgtgcgc cggagcctgg
      841 tgtagaaacc ggcgtccgta cgactgcgct cgatcagccg cgccagcgcc ttgtcgtcga
      901 acaacggaaa gtagccgggg tagtcacggc ccagcatgcc gatgtttccc ggaacgcgcg
      961 aggcgagcac cgggaccccg atgcgggccg cttcacagat tacgtttgct ccgccctcca
     1021 tcaccgagct gaccaccagc acgtggctgg aggcaagcca gcgcatcgct ctccggtgag
     1081 gaaggcttcc cagccaccgg tagcgggact cgcacctcat ccatgccttg caccgcgccc
     1141 gcatttccgg atccaacgcg gcgccgatct gcaatacctc gaccggggcg gaccggtcga
     1201 ttcggcgaag ggtcatgacc gcgcgaaagg gatctttctc gtcccgcaag tggccaatga
     1261 ccgcaatgcg gaaccgcgac agctccggtg cgtgtcgcag cgtcacgtcg gaggattgat
     1321 agacgacgct ggtcttgcgc cgtaggaacg ggcttagctc gcgcaggccc tcctcctgca
     1381 gcacgacgag ccgtgcggcc agtcccagcg actgcctggc ttccgggtat tttccgatgt
     1441 cgcggtagag gtcggtcccg gtcagcgcga ccacgagggg cgcatccggc cgcaggtccg
     1501 agaaccgccg gatggaggca aagctcttgc gagcatgcag cgcgagcagc aggtcatcat
     1561 tgccgctccg ccattccgtc acgatacgca cacgatgccc cagctcgcgc aggaagcgcg
     1621 cccagcgtgc ggcggtgtgc cggttgccgc tgcgcggcac cgccgagggc ggaacggtga
     1681 tggcgatttt catatgccgg ctattccctt agacttgtaa cggcagcttt acccgcttgt
     1741 tccaaccgac gaacgcataa tggaacccgg ttctctcgcc ggcgagctgc gcgccagccg
     1801 cgcccatctt actcgaatca ccgccgatct cgacggtgac cagttgctgg gacccaggct
     1861 cgcgatcgtc aatcctcccc tgtgggaact cgggcacgtg gcctggtttc aggagtactg
     1921 gtgcctgcgt cgcagcacgc tgggaaaaac cgtcgactcg gtcgtgccgg gcgcggacgc
     1981 tctctacgat tccgccaagg ttgcgcacga cacgcgctgg gatctgcccc tgcccggcct
     2041 gaaatcgacc ctgtcctatc aggccgaaat tctggagcgc gtgatccggc gtctggggaa
     2101 ggaaccggaa aatcgcgagt tgcagtactt tgtacagctc gcgattttcc acgaagacat
     2161 gcacgcggaa gcctttcact acacccgcca gacattgggt tacgaagatc cgttccacaa
     2221 taacgaaatc gcgcccagcg ctcacgaacg cgtgcatggc gacgtgtccc ttcccggcgg
     2281 cacctttctc ctgggggcat cgccagggcg cgatttcgtt ttcgacaacg agaaatgggc
     2341 gcatgaggtc cggctccgtc cgtttcgcat atcgcgcacg ccggtcacca acgcggagta
     2401 cgtcaaattc gtggaatcgg gcggctactc ccggcgggag tggtggagcg atgacggctg
     2461 ggcgtggaaa tcccgcgagg gcttgcacgc gcccaagtac tgggccaagc gcgacggcgc
     2521 atgggcgcaa cgaagatttg accgcgtcat cgcgctgcgc ccggacgaac ccgtcatcca
     2581 cgtaagctgg cacgaggcgc aggcttattg ccgcttcgcc aggcggcggc tgccgacgga
     2641 agcggaatgg gagtacgccg ccgcgtggga tccccacgcc ggtggaaaaa aacactattc
     2701 ctggggtaac cagttacccg acgcagcccg tgcaaatctg gaaagcgcgg gcatcgccgg
     2761 cgtcgacgcg tatccgacgg gtgatactgc gtcgggttgc aggcaactga taggaaacgt
     2821 ctgggaatgg accgagagca cgttcaaccc ttaccccgga ttcatcattg atccgtacca
     2881 ggagtactcg caaccgtggt tcgggacgca caaggttctg cgcggcggca gcttcgcgac
     2941 cacccgccgc ctcatccgca atacctggcg aaacttctat acgcccgatc gctatgacgt
     3001 tttcgccggg ttccgcacgt gcgcagccga atgagcccgc gcggccgcag aagattcatc
     3061 ctcggcgcgg cgggaatagc gggcgcagcg gctctcgccg ccgggttcct gcgatcgcac
     3121 cgcccgcggc tggatgaact taccgcgcgc ttcgagccgc cgctgcctcc cgatccggcg
     3181 ttccccaacc ctctgaggtt gccgggcgcc gaaggcatgt tcggcgtggc ggaggcggcg
     3241 ggtgcgctca cgatcatcgc caaaggcatc gaatatccga ttctgccggg caagcctgcg
     3301 cgcatgcttg cctacgaaat ggaacaccac ggcaggacgc tgctcaatcc ggtgttgcgg
     3361 gcgcgcagcg gcgcgcagct caggatcaaa ttctggaacg ggctggagga gaccagcatc
     3421 attcactggc acggcctcaa agtggacagc aacaacgacg gccatccgca ttacgccgtg
     3481 cccgggggcg ccacctacga ctaccagttc accgtcgcca atcgcgcggc gacctattgg
     3541 taccatcccc acccgcacca tctgaccggc aagcaggtct atcacggcct ggcgggtttc
     3601 ttcatcgtcg aggatgatga agaacttgcg ctgcagaaat cgctcgacct caggttcggt
     3661 gaaaccgaca ttccgctggt cattcaggac cggcggctgg atgaaaacgg ccgattgatc
     3721 ttcacgccca cccccgatga gcgattccac ggccaatacg gcgaccaggt gctcgtcaac
     3781 tggacgccgc gcccccattt ccacgcggcg acccgcttcc atcgcttccg catcctcaat
     3841 ggctccaacg cccgcctcta tcgcctcgcg tttcgtcacg gcgagcggct gctcgattac
     3901 gcggtaattg gcacggatgg cggcctgctc gaccggccgc gcagcgtccg cgaggcgttc
     3961 ctcgcgccgg gcgagcgcat cgacgtgctg ctcgacttgc gcgccgccgc ccgcggcgat
     4021 gcggtgacgc tagtcaacct gcccttcgac gcgatgcaca cggagacgaa gcgcgcgcag
     4081 ggctcacatg aagagcacgc ggcgcatgcc gcaacgccgg acggcgcaga ggccgagctg
     4141 ctgcggatcc gggtccagag caagacccgt tacgacagga cccttccgca gacgctttca
     4201 cgcatcgagc cggtcgcgga gagtccctac agcccgcgtc tcatcgtgct cgaccaggcg
     4261 aagatggcgt ggcgcatcaa cgcggcgacg tacgacgtgg ccgcgacgcc gatcaccgtg
     4321 cggcgcgaca gcgtcgaagc ctgggacata cgcaacgttc cgccgacgat gccgcacccg
     4381 atgcacatcc acggcttcca gtttcgcgtc gtctcgcgcg aaggcagccc ggaacagcag
     4441 cggcgcttgg ccgtccaaaa gagcggcctt gccgcatccg acctcggctg gaaggacacc
     4501 gtgctggtgt ggccgggcga gaccgtgcgc atcgtgaccg acttctcgca tcctttcctc
     4561 ggcgaccagg tctacatggt ccactgccac aacctcgagc acgaggacag cggcatgatg
     4621 ctcaacctca aagtgacggc ataactggct cgtggaccca aaacccgtga aatcgcgaga
     4681 tcggaaggac gtgagatcga gcttatttcc ctctcccact ctcgcgccct gacgccctca
     4741 cggctttcag gacgccccgt cttcggaagg tttgacgcag atcaaacctc gcgcgtcggc
     4801 tgtggcgcct aattgccctg agtgtttggt tgaaacacgg caaaggagac aaatgatgag
     4861 aacaatgttg cgctcgattc tgctggctgc ggcgttgacg atcccggctt tcccgggcca
     4921 tgccgcggaa gacatcaccg tctacaagga cgccacctga gggtgctgcg gctcgtggat
     4981 agatcatctg cgagcgaacg gcttccgtgt gaagccccag aatgtcatta atcccgaagc
     5041 ctaccgtgcg cgcttcggca taccgaacgc gttcggctcc tgccataccg ccgtgatcga
     5101 aggttacgcg atcgagggcc acgtgccggc gcgggaaatc caacggctcc tcgccgagcg
     5161 gccgaaggcg cgcgggctcg cggtgccggg catggcggcc ggctctccgg gaatggaagg
     5221 cagccgcagc gtgccgtacg acgtgctact cgttcttccg gacgggagct acagggtgta
     5281 cgcgcgctac ggacgatagt cagtcttgaa ctttcgcgct cgtccccggg caagctgtga
     5341 tggcggggcc ggtcgtgccg cgatacatgc gagccatgaa acctccgcgg cttgccgttc
     5401 tggtcctggc cttgctgttc gctgccgcgg acgccccgtc cgcggaaccg gtcgcgccgg
     5461 atgcgctcac tacaacgatg cggcccgtct caccgggccg ggaccacgtt ttcggaaacc
     5521 ccagggccga gatttccctg atcgagtact cggatttcga gtgcccgttc tgcaaacgct
     5581 tccacgaaac ggcgaagcag gtcgtgcagg cgtacggcgg cagggtgaac tgggtgtatc
     5641 gccatttccc gctccccttc cataatccgg cagcgcaacg cgaggccgag gccgcggagt
     5701 gcgccgccga gctcggcggc aacaacgcat tctggcgcta cgctgatctg atcttcgggc
     5761 gcaccccgtt gaatggacag ggcatcctgg aaaaagagct gcacgagatg gcggcgtcca
     5821 tcggactcga tcgcgcgaga ttcgcggcgt gcctgcgtag cggccgcaag gcgctgcgcg
     5881 tcgacgacga tttcatcgag ggccaggcga tcggcatcag gggaacgccg ggcaacgttc
     5941 tgcgcaacaa ccgcaccgga gcaatcatgc tgcgccaggg tgccgtgccg ctcgagcaga
     6001 tcaaaggcgt gatcgatcag ttatcgaagc aaaaatagcg acccaacacg cacaagcggt
     6061 gccgaatggg ttcagccatg agcgaggaac ccaatatcag gccggcgacg aagagcgata
     6121 ctatgtccag tctgcgaaac agggttggcg tgggcctcct gacggcttcc tttgcgtttc
     6181 tcgtcggcgc gattgtctac gcgctctgga gcggggagaa accgcttgct gatccagaca
     6241 acccgcggca ggttgcgcgc ggcaagccgg tgtacgaccg gcactgcgtg tcctgccatg
     6301 gcgtgcgcct cgagggccag cacaactggc aggaacgctt gccgagcggg cgctatccgg
     6361 caccgccaca cgacttctcg gggcatactt ggcaccaccc ggacagtatg ttgttcggga
     6421 tgaccaagta tgggctcgtc cccggcaagt acgcaccacc gaaatacgag agcgacatgc
     6481 ccgcgttcgg cggcgcacta tccgacgagg aaatctgggc agtgctggcc tacatcaaat
     6541 cgtcctggcc atcggacatc ctcaaggcgc agcacgagat gacacgcgag cgggatcggc
     6601 gctgagccag ccgcgcttga tctagatcaa cctccactca atgcctagat atatgattgt
     6661 ccagactatt cacgattgtc cgcaccccct gcagaacgtt gtggggtgcg gctgatcata
     6721 gtcaagtaca tgaacatgtt ttcgtcaaca atgaactcgc catccgccgc gggccggacc
     6781 gccagtgaga ggaaacggtt tgtagtcccg ttgctggtcg ggtggtctgc attctggctc
     6841 gtcagcatca tcgcgccttg ttgtgaaagt ctcataacca aggcgcaagc cggacaggaa
     6901 ctggccgcgc ttcaatacgt tattcaatgc ccggccggca gtgaccagcg cgaaatgccg
     6961 tgcccgggtt acatcgatgt gcaggcggcg ccatccgcgt tgacagtcac gtcattcgat
     7021 tcgacgcctc gcattccggg gagccccctg cacgcaacgg ttccgttcgt tctgagcccc
     7081 gctgaatctg cgaagcactt cctcggcgcc cggccaccgg caccgattcc gttcctcctt
     7141 cgcacctcgc gtctgctgat ttaacccccc gtttccggag gcagtgcttc cggttgcgca
     7201 agcacgtacg ctccattccg aggctgcgac ttgcatagca caccgtaatc ctgccatcgg
     7261 gaaccggcag tcgtttctga ttccagaatt ggcaatagca acttggttca accagttaaa
     7321 ggagaaaccg aaatgaaacg ctcgattctt tgtgccgcag tcgctgctat cgccctctct
     7381 gcgccaatcg ctttggccca ggacaccaag cccgccgccg gcaaaccggc ggcaatggaa
     7441 atggacaagc agatggcgca gatgcaagag aacatgaaaa agatgcagca gcagatggac
     7501 aagattcgcc aggccaagga cccgaaagag cgccagaagc tcttgcagga gcacatgcag
     7561 agcatgcagg aaggcatgaa catgatgcgc ggcatgggcg gcccgatgat gatgggcgga
     7621 cagggcggta tgggccccgg catgatgggc gggggtcaga tgggcggcgg ccagaagggc
     7681 ggcatgggcc ccggcatgat gggcggcggg ccgatgacgg gcatggaccc gaaacagcag
     7741 caggacttca tgcagcggcg catggacatg atgcagatga tgatggagca catgatgcag
     7801 catcagcaga tgatgcaacc ggcgccggcg aagtagcgga caaataccac catgaataca
     7861 tacgcaatcc ccgccgcgcg gccgaccggt ttcggagtgc tcgccttcgg cctgctgctc
     7921 gcggcgtatt tcgccgtgct cacgctggtc tccgactgga agttcaccgt ggaccagttt
     7981 tccgagttct ggttttacgt cctgccgctg gccgccggat tcgggctgca ggttgcgctc
     8041 tacgttcgcc tgcggcaact actgcaccgg tcaaaggatg cgggcacggt aatggcggcc
     8101 tccggcacta cgtcgaccgc ggcgatggtc tcctgctgcg cgcactacct ggtcaacgtc
     8161 gcgccggtgc tcggcgcgac cggcctcgtg accttcgcca cccagtatca ggtcgagttc
     8221 ttctgggttg gcctcgcgtt caacgtcgcc ggcatcgcct acatcgggaa caaactctgg
     8281 aacgcaacca aggagcacgc ccaatgcgca catgcctgac cgtcttcttt tcaggaattc
     8341 tcatgaccct actcgtgccg ttccaactta tttctccagg tttgggcggc ttgcctgcta
     8401 acaacgatac agccgagcag aaccagtttc ttttcccgca aatagttgga acggcactcg
     8461 cagccggcgc cttcgccgcc gagctgggca ttcaacgcag cagcgccaac ggggtgaccg
     8521 tggcggccac cccgcaaaac ctcgccgcga gcgccaagag ctgggacttc aaggtggcgc
     8581 tcgacactca ttcgggtgaa ttgaaggacg acttggtgaa aagcgccgtg ctcgtagtcg
     8641 acggcggcgc ctcctacacg ccgacagcgt ggcaaggcga tccacccggc ggtcaccatc
     8701 gcgaaggcac gctcagtttc aacgcgattg caccgcaacc caaagcgatc gagctgcaaa
     8761 ttcagcgccc gggcgaaccc aagccgcgct cgttccgctg gcaacttaaa tgaaaagaga
     8821 gcattgatgt acggattcag cacccgcctc aaagtgccgt tcgactccgc tgtcgagaaa
     8881 gtgaccgcgg ccctgaagac ggaaggcttc ggcgtgctca ccgacatcga cgtcaaggcc
     8941 actatgaaaa ccaagctcaa tatcgagcgg cgcccatacc gcatcctggg cgcgtgcaat
     9001 ccgccgctcg cgcaccgcgc gatcgaggcg gatcccgaca tcggcttgct cctgccgtgc
     9061 aatgttgtcg tgcgcgagga agacgacggg cgcatctggg tcggattcat ggacccggaa
     9121 gccgtgctca agctggtgga caagccggaa gtgcatacgc tcggcaagga agtacgggcg
     9181 cgtctgcaac gggtttgcaa cagcctcggg tcatgaccag aaggaggcac tcatggacac
     9241 ggaaactctg aaaagcctgg gcagcttgct cctctggggt ttgctgtttt tcctgatgat
     9301 gcgcttcggg tgcggagccc acacgatggg tgggcatggg catggcggtc atggcagcca
     9361 tggcggaagc ggcggggaaa aggacccggt atgcggcatg gccgtcgatc cggaaaaggc
     9421 agcgggcgcg agcgtgcacg cgggcaagac gtattacttc tgctccactt cttgccgcga
     9481 cgagttcgag aaagagccgg cgaaatacgc cgccgctcct gcgcgcgacg aacatcagca
     9541 tggaggacac tgccatgggt aggctctact ggcgcgatgt cgcgctcgcg atgggctcgt
     9601 tcctggccat tacctacgta ttctgtgtcg cctacgacct ggcgttcggt cagcggatgt
     9661 acgaggtctg gctcaagctg ctgccggggt tcacttggat cagctggccg agtttcttcc
     9721 tcgggctcgt cgagagcttc ctgtacggca tttacttcgg cctggtattc gtgccgcttt
     9781 acaacttttt ccatggcaag tgacggctgc ggcgcgacgc gaacaggctg cgaccctcac
     9841 atttcgcaat tgggcaaagg aggctcgtat ggatacaacc aaaccctggg ttacaggcgc
     9901 ggcgcttgcc gtcaccgcgg gcgtcgtcta cctcgtgtgc gcggtggcgg tggcactgtt
     9961 tccggatggc acgctggcgt ttttcagcac ttgggcgcac ggcgtggatc tgacgctcgt
    10021 caggcgtccc gccgcaaagc ccctcacggc agaagagtgg atttacggct ttgtgtctat
    10081 cgtggtggca agctatctcg ccggtgcgct cttcggttgg gcacgcaatc tgttcagcgc
    10141 ctggaaatag aagctcacag atgaaaccgt cgcgcgcaac agcttttctt gcggcgtccg
    10201 ccgcggtgtc ggtcggtgat cgcggtgtcg tgtacgccac tgacgagcgc acgagcgcgt
    10261 catcgaatga acccggccag cggattgcac gccatggttg agcttccgca cacaagcagc
    10321 gccgaacacg tgtgccgtca ctgtggcgcg cccatttcgg gcgtgccgta cgtgcgcgag
    10381 cacgacgaat acggttgcga aagctgtttt ctcgcctcac gcaagcggcg gacgctcccg
    10441 ggcggagttg atgaaacgca gttcgcactc tcggaagcgc tggtgtgcgc gctcgacgcg
    10501 cgcgaacacg aaaccggcca gcactcgaaa cgggtcgctt gccacacgct ggtgctggcg
    10561 cgtcgcttca cggctgactc gcaacagctg cggcagacat actggggcgc cctgctgcac
    10621 gacatcggca agatcggcat cccggactcg atcctgctga agcaggacgg gttgaccgcc
    10681 accgaatgga agatcatgcg cacccacccc gaaatcggcc accggattct ctccggcgcg
    10741 ccgttcatgg ccgaggcggc cgagatcgtg cttgcgcatg aagagcgctt cgacggaacc
    10801 gggtatccgc gcggactggc gggagcggcg atacccttgt gggcgcgcct gttcgcggtg
    10861 atcgacacgc tggacgccat gacctcggac cggccgtacc gcagaggtct tccattcgag
    10921 gccgcccggg cagaaatcgc gcgccaatcc ggcacacagt tcgatccgca ggccgtggag
    10981 gtcttcatgg cggaggacgc ggcgttgcgc gagatggtgg cgctcaaatg cggcgcaaca
    11041 gccatcccgg atgcggtgcc ggtctaaacg gtcaagccgg attacctgga gcaaagtcat
    11101 gagttacgtc gagacaccag cggactttcc ttacccgtgg tacctccggc tgatgttccg
    11161 gcggcagcgc cggcgctacg ggcgggagct cgagccggtc cgcctgtggg cgcggatgcc
    11221 ggcggtgttc ctcgccatga gcgcgatgta ccgggcgctc gatcgcaagt catcgcgcat
    11281 cgagcctggg ctgagatcgc tcatccaggt gcgggtctct cagatcaacg gctgtgaatt
    11341 ctgcgtggat ctgaactcgt tcctcggtct cggtcgcggt atcgcggagg acaagct
//
LOCUS       DBNGWZ010000023         1871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2017340, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000023 DBNGWZ010000000
VERSION     DBNGWZ010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2017340"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="AC16CX_00630"
     CDS             complement(<1..1025)
                     /locus_tag="AC16CX_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011047.1"
                     /translation="MRCRELPVQPVTLGLVLAVAAGVLLGEWFFAQAQDELLAERQER
                     EVVMQTMALADLVAAVEKTAQAGASTLAALASEWAARHGEIAAIRVVRSGDARLLAST
                     DPKDARPQPPPRRLERAEKRLYDLGHELRANVETNAAEGVFRKKQVDVARLDRAALRV
                     SVPYAVGGRVVGFVQVEQRVGAPPAPPVAGRTAVAIAAPLALFLLVARLLQGRAPAAG
                     RPWVPYGIAVLFLGAALYGAARDGLADLDTRQELFAAELARHYVAIKGEVQSAAATHA
                     LAVEAVTDNRWDVDDYQRPRAVISGDGTIARAALAAELDARDTRVARSLWGNAGLSLL
                     LLSFVGLG"
     gene            complement(1022..>1871)
                     /locus_tag="AC16CX_00635"
     CDS             complement(1022..>1871)
                     /locus_tag="AC16CX_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3011048.1"
                     /translation="MTYRDTVYGLPLNYKSITLIYNKKLVETPPKTSGELVKLAKRLT
                     DAAAGRFGLAYAYNDFYYHAALQNAFGGRAFDPGPKPVLNAPQNVAAMKLLLKWMRQD
                     QILPAEPSTALVTSLFNEGKAAMVFSGPWFLGEIAPGIDYGLALLPTIDEAGGKPMRP
                     WLTIEGVYVSASSRQKEAAYELAKYLVSEEAGLVLAVEGRQLHTNKAVYTHKAVADDP
                     VLGAFRRQLDTATPMPNFAEMTVMWSPATTAMNTIVRGSATPEVALEKAQRTVEKDVA
                     ALRKGR"
BASE COUNT          269 a          696 c          617 g          289 t
ORIGIN      
        1 ccgaggccga cgaaactgag cagcagcagg ctgagtcccg cgttgcccca caggctgcgc
       61 gccacccgcg tgtcgcgcgc gtcgagctcg gccgcgagcg cggcgcgggc gatggtgccg
      121 tcaccgctga tgactgcgcg cggccgctgg tagtcgtcca cgtcccagcg gttgtccgtt
      181 accgcctcga cggcgagcgc gtgcgtggcc gccgcgctct ggacctcgcc cttgattgcc
      241 acatagtgcc gcgccaactc cgccgcgaac agctcttgcc gcgtgtcgag atcggcgagc
      301 ccgtcgcgcg ccgcgccgta gagcgccgcg ccgaggaaca gcaccgcgat gccgtacggc
      361 acccacggcc tgcccgctgc cggggcacga ccttgaagca gccgggcaac cagcaggaac
      421 agtgcaagcg gcgcggcgat cgcgaccgcc gtccggccgg cgaccggcgg cgccggcggc
      481 gcgccgacac gctgctccac ctgtacgaag ccgaccaccc tgccgcccac ggcgtacggg
      541 accgacaccc gcagcgcggc gcggtcgagg cgcgcgacgt caacctgttt cttgcggaag
      601 acgccctccg cggcgttcgt ctcgacgttg gcgcgcagct cgtgcccgag gtcgtagagc
      661 cgcttctccg cgcgctccag ccgccgcggc ggcggctgcg gccgcgcgtc cttcggatcg
      721 gtcgaggcca gcaaccgcgc gtcgccggag cgcaccacgc ggatcgcagc aatctcgccg
      781 tgtcgcgccg cccattcgct cgcgagcgcg gcgagcgtcg acgcgcccgc ctgcgccgtc
      841 ttctccacgg ccgcgacgag atcggcgagc gccatcgtct gcatcaccac ctcgcgctcc
      901 tggcgctccg cgagcagctc gtcctgcgcc tgggcgaaga accactcccc cagcagcacg
      961 cccgcggcca ccgccagcac gaggccgagc gtgacgggct gcacggggag ctcacgacat
     1021 ctcatcggcc ctttctcagc gcggcaacgt ccttctcgac cgtgcgctgc gccttctcga
     1081 gcgccacctc cggcgtcgcg ctgccgcgca cgatcgtgtt catggcggtg gtggccggcg
     1141 accacatcac cgtcatctcg gcaaagttcg gcatcggggt ggcggtgtca agctgcctgc
     1201 ggaaggcgcc gagcaccggg tcatcggcca cggccttgtg cgtgtacacc gccttgttgg
     1261 tgtgcagttg ccgcccttcc actgccagca ctaagcccgc ctcttccgac accaggtact
     1321 tggcgagttc ataggccgcc tccttctgtc tggaactcgc cgacacgtac acgccttcaa
     1381 tggtgagcca gggccgcatc ggcttgcctc cggcctcgtc aatggtcggc agcagcgcga
     1441 gcccgtagtc aatgcctggc gcgatctcgc ccaggaacca cggtcccgag aacaccatcg
     1501 ccgccttgcc ctcgttaaac agcgacgtaa ccagcgcggt ggacggctcg gccggcagga
     1561 tctggtcctg gcgcatccac ttcagcagca gcttcatggc cgcgacgttc tgcggcgcgt
     1621 tcagcaccgg cttcggaccc ggatcgaacg cgcgcccacc gaaggcgttc tggagcgcgg
     1681 cgtggtagta gaagtcgttg tacgcgtacg ccaggccgaa gcggccggcc gccgcgtcgg
     1741 tgagacgttt ggcgagcttg accagctcgc ccgaagtctt cggcggcgtc tcgacgagct
     1801 tcttgttgta gatgagcgtg atgctcttgt aattgagcgg cagaccgtac acggtgtcgc
     1861 ggtaggtcat c
//
LOCUS       DBNGWZ010000024         1709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3024946, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000024 DBNGWZ010000000
VERSION     DBNGWZ010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3024946"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..814)
                     /gene="fnr"
                     /locus_tag="AC16CX_00640"
     CDS             complement(26..814)
                     /gene="fnr"
                     /locus_tag="AC16CX_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006767385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR3011049.1"
                     /translation="MLQSIALSQPIPVMPAKARSTTVVRLAPQVRSAAACPSCCLKGV
                     CLPCDLGRAELDRFDEITAGKRRVARGAALYHNGDHFESLYAVRSGSFKTVGVSRQGV
                     EKITGFHLPGELLGLEAINGGRHGYSAVALEDSEACIIPFAQLERMAMSVPALQHQLL
                     RLLSSDISRDHGLMLLLGSMTAEQRLAAFLLSLSRRHQRLGFSANRFVLRMTREEAGN
                     YLGLTLETVSRLLSRFQREGLVSVQQRDIEIKNADRLMEMVGHW"
     gene            complement(871..1284)
                     /locus_tag="AC16CX_00645"
     CDS             complement(871..1284)
                     /locus_tag="AC16CX_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3011050.1"
                     /translation="MGYYNYMAGRDMKVFVVEDSAPVRERLIDMVREIEDVDVVGEAG
                     TYGAAVAGIMNTRPDVAILDIRLADDAGSGIDVLNEVRKGLPTIKAIILSNYATPQHL
                     KASADAGAEYFLDKSRDFERIPGILKEMRGEAGAR"
BASE COUNT          327 a          533 c          539 g          310 t
ORIGIN      
        1 gttcaccgtt cacggatcac ggttgttacc agtggccaac catctccatc aggcggtccg
       61 cgttcttgat ctcgatgtcg cgctgttgca cagagacaag cccttcacgc tggaaccttg
      121 acaggaggcg gctcaccgtc tccagggtca gcccgaggta gttgccggcc tcctcgcgcg
      181 tcatgcgcag cacgaagcgg ttggccgaga agcccagccg ctggtgccgc cgcgagagtg
      241 acaggagaaa cgccgcgagc cgctgctcgg cggtcatgct gcccagcaac agcatcaatc
      301 catggtcgcg cgagatatcg ctggaaagca ggcgcaacag ctggtgctgc aacgcgggca
      361 cggacatcgc cattctctcc agctgcgcga agggaatgat gcacgcttcg ctgtcctcga
      421 gcgcgaccgc gctgtagccg tggcggccgc cattgatcgc ctcgagaccg aggagttcgc
      481 ccggcaggtg gaagccggtg atcttctcga cgccctggcg cgagacgccc accgtcttga
      541 acgagccgct gcgcaccgcg tagagcgact cgaagtggtc gccgttgtgg tagagcgccg
      601 cgccgcgcgc cacgcgccgc ttgccggcgg tgatctcgtc gaaacgatcg agctcggccc
      661 gcccgagatc gcacggcagg cagacgccct tcaggcagca ggacgggcac gccgccgcgg
      721 acctaacctg cggcgcaagc cgcacgacag tggtgctccg ggccttcgcg ggcatgaccg
      781 ggatcggctg ggataacgca atggattgca gcatggtgat ctcctgtact aactaaggtt
      841 tgcccacacg agaagcgcgc ggtgcccgca tcagcgggcg cccgcctcgc ccctcatctc
      901 cttcaggatt ccaggaatgc gctcgaaatc ccgcgatttg tcgagaaagt actcggcgcc
      961 ggcgtcggcg ctcgccttca ggtgctgcgg cgtggcgtag ttgctcaaga tgatcgcctt
     1021 gatcgtgggc aatcccttcc tgacctcatt gagcacgtcg atgccgctgc cggcgtcatc
     1081 cgcaagcctg atgtcgagga tcgctacgtc cggccgggtg ttcatgatgc cggcgacggc
     1141 cgcaccgtaa gtgccggcct cacccaccac gtccacgtct tcgatctccc tgaccatgtc
     1201 gatcagccgc tcccgcacgg gcgcggaatc ctccaccacg aagactttca tatcgcggcc
     1261 cgccatgtag ttgtagtagc ccatggccgg cagtatctgc gggcggccgc cagcgcacta
     1321 tcggcgcgag tctgaaaacg actgtaggaa aagaggaggc ggtgtaggaa acgtccgaca
     1381 gtatgtactt acgtaaaggg ttgtcgggac taggcgggat gaggcgggac taagcgaaac
     1441 ccattgaaat aagctagaaa acgctatgtg ttaccgtacc ccaagagggg gtatgggtca
     1501 tgcactactg gcgatctgtt attagggaag ctaataaggc cagataggtg ataagagcca
     1561 gctaccgcgt gagcgcaggt aacctgtcac ttatcactta tcactcgtca ccttgacgag
     1621 ccgggcggtt cgtcaaggag ctggtgctcg agggcgtagc ggatcagctc ggcctggttg
     1681 gcgagaccca ttttctccag gatgcgcgt
//
LOCUS       DBNGWZ010000025         5829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3025024, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000025 DBNGWZ010000000
VERSION     DBNGWZ010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3025024"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..321
                     /locus_tag="AC16CX_00650"
     CDS             148..321
                     /locus_tag="AC16CX_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011051.1"
                     /translation="MRSLIAGLLAVAMLGGCVAVPYYPEPAPAGYYYYGPVYPPATVY
                     FRYDYYRGSRHRW"
     gene            379..729
                     /locus_tag="AC16CX_00655"
     CDS             379..729
                     /locus_tag="AC16CX_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HIR3011052.1"
                     /translation="MRARNLLSCLLIAATTSLASAASLAESRVSIGISIGPPPPPVVV
                     APAPVPGYVWAPGYWAWNGYQHVWIEGYWIAARPGYYWVPDRWERRGPYWHHVHGHWG
                     PRFHDRGHRRGHRH"
     gene            complement(1448..1876)
                     /locus_tag="AC16CX_00660"
     CDS             complement(1448..1876)
                     /locus_tag="AC16CX_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3011053.1"
                     /translation="MVNITRFNPYEDAIENLFRGVPVWVPNLETRAPAPTQFRMDVTE
                     NDKEYQVLAEMPGVKKEEISVTINGSEVAVSAEVRHEKDIKNGGTVLRAERYYGKIQR
                     EFTFSQEIDEAKAQAKYNDGVLELTLPKKSGAAAKRLAVH"
     gene            complement(2619..2981)
                     /locus_tag="AC16CX_00665"
     CDS             complement(2619..2981)
                     /locus_tag="AC16CX_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011054.1"
                     /translation="MTITPETDAKVKFGIWGFVLGAGVAMTIGFVWGGWTTSSTTQKM
                     SEEAVLTSKVAICVAQYMNSPNHKINIKEFQGTESYQRSELIEKGGWDKMPGEEKAAW
                     GVSSACVAGLEARIKTGA"
     gene            complement(3636..4079)
                     /locus_tag="AC16CX_00670"
     CDS             complement(3636..4079)
                     /locus_tag="AC16CX_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HIR3011055.1"
                     /translation="MGEKAEVARPRRVVLDTNVAVSALVFREGRLAWLREAWEAGRIV
                     PLVSAETLAELVRVLAYPKLKLSEEDTKNVLAHYMEHAEAVREGGASVRVPECRDPDD
                     RKFLQLAYAAKADALVTGDEDLLALAGRSRVPILTPDALKAELRT"
     gene            complement(4058..4282)
                     /locus_tag="AC16CX_00675"
     CDS             complement(4058..4282)
                     /locus_tag="AC16CX_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3011056.1"
                     /translation="MLAKLTSKNQLTLPKAVVGQFPGARYFEVRAEKGRIVLVPAKVT
                     SLEGVWRKIESLGITEKDVEGAVRWARKRK"
     gene            complement(4501..5754)
                     /locus_tag="AC16CX_00680"
     CDS             complement(4501..5754)
                     /locus_tag="AC16CX_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HIR3011057.1"
                     /translation="MREAHQDAVNRVDQQRLWRRLMEMAQIGAIPGGGVNRAAFSKED
                     IAARKLLISWARARNFAVAMDAIGNLFIRRAGTDPRAAPVMTGSHMDSQPRGGRFDGI
                     YGVLAGLEALEAIEQAGVKTRHPVELVAWSNEEGGRFQPCTMGSAVYTGAKPLGDFLE
                     VKDNEGVALEDALAQTLAATPAAEQRELNAPAAAYIEAHIEQGPLLEQEGRTIGVVTG
                     IQGLRWFNVEVFGETAHAGTTPLAGRKDALREAIAAINALHELTRDASDTLRFTIGRV
                     LVTPNSPNSVPSHVMFSVDVRHPDAATIARLGEAVEPAVRKAVTRCSVKVTPTLHDDP
                     CAFDPAVVDCVERAAQALKLAHQRMPSGASHDAMYMAQVCPTGMIFVPCERGISHNEA
                     ENARPEDLAAGARALTAALLELADQ"
BASE COUNT         1005 a         1869 c         1759 g         1196 t
ORIGIN      
        1 cccggcgccg ctgtcgtcgt cgggcgacgc tgacgcgctc tcccggtaac aaacgcttac
       61 atcttcttac gcactgtagg catttgcgcg ggtctccgct tgtcgtaccg atcaggacga
      121 tcctcgaggt tcgtgaagga ggtgcctatg agaagcctga tagctggctt gctggcggtc
      181 gcgatgctgg gcggctgcgt ggccgtgccg tactacccgg agcccgcccc cgccggttac
      241 tactattacg ggccggtcta cccgccggct accgtctact tccgctacga ctactaccgg
      301 ggctcgcgcc atcgctggta ggcgccgcgc cggcggaacg cggatgaagt ctgtcgcgct
      361 gaaaaaagga ggtacatcat gcgtgcgaga aatctgctgt cgtgcctgtt gatcgccgca
      421 acaacgtcgc tggcatcggc cgcaagcctg gccgagtccc gcgtcagtat cggcatctcg
      481 atcggtccgc cgccgcctcc tgtcgtcgtg gccccggcac cggtgccggg ctacgtgtgg
      541 gcgccgggat actgggcctg gaacggttac cagcacgtct ggatcgaggg ttactggatc
      601 gcggcgcgcc cgggctatta ctgggtgccc gaccgctggg agcggagagg tccgtactgg
      661 catcacgtgc acggtcactg gggcccccgg ttccatgacc gcgggcaccg ccggggccat
      721 cgtcactgag gcttgcgcgc gagaaaaagg gtcaggaccc ttttttctgt ttcaagccag
      781 accagcggct cttcgccctc gtaacttgga acttgaaacc ggcggccaac tcttagagga
      841 cagaccacta acgaccgcgc acccactcac ggcgcattgc gcttgggtgg tctctggtgg
      901 aatttcctca ccaacctctt atcgcgatgt ggcccgccgc cgggtctgag atggccgcca
      961 ggcgcaggag tgttcccccg agcgccctgc tgcggagctc cctcccgatt tccggtggcc
     1021 agcaagatct cgagctcatt gagctcatcc cactgggcat cggtccgctg cctgtccgga
     1081 atcgccagaa gcgcttgcag gcgggcacgg ggggaagccg gttgcggatc attcatggcg
     1141 gcattatctc atcgttcgat aaagggccca aatgagaaat gggtgaagag gcgcctgagg
     1201 gatgagggcg ttttgattag tgaaatgcgg attgtgaaaa tcgctccgac tccattttca
     1261 cccatccttc gggtagcccg tcacccgtat ctctattgct ctggccgcag cacccactac
     1321 caacaaaaac cccgccgaag cggggtctgt gtatctacga ctcaattgca tgtggcggaa
     1381 tattcaacat ctcagacggc cacattgatt ccgcggtcga acctattcgt gctattcgag
     1441 gcgcggctca atgcaccgcg agccttttcg ctgccgctcc acttttcttc ggcagcgtca
     1501 gttccagcac gccgtcgttg tacttggctt gggccttcgc ttcgtcgatc tcctggctga
     1561 aagtgaactc gcgctggatc ttgccgtagt agcgctcggc gcgcagcacc gtcccgccgt
     1621 tcttgatgtc tttttcgtgc ctcacttccg cggataccgc gacttcgctg ccgttgatcg
     1681 tgacgctgat ttcttccttc ttgacgccgg gcatttcggc gagcacctgg tactccttgt
     1741 cgttctcggt tacgtccatg cggaactgcg tcggcgcggg tgcacgcgtc tcaaggttgg
     1801 gcacccacac ggggacgcca cggaacaagt tctcgattgc atcctcgtac ggattaaagc
     1861 gtgtgatatt taccattttg gtctccaata aaaattcaag ccaatcggcg atgaatgact
     1921 gtgcgtcgca tccgttgccc atttagttgt acatgggggc gccgccgcga ctatcaagct
     1981 cgcgagcctg aatatttcac gtagtaaatg agtaaatggg gtcacccagt gcagctttac
     2041 cgtcatccgg gacgcccgcc ctccccaacc gctagctcct ggattactga tcggacgagt
     2101 cgccctcgtt tcgtgcaaaa aactaaaggg tcagactcga tcttgtggcc ctttggcggc
     2161 gggatgcgtt aaggagaggg aagcagaatc ggtgggcaag aacgcaaata cccgtcaaac
     2221 taacgtgcca tgccaacgtt cgacaaccgg aggttccatt gagtggtcgc agccaaacgg
     2281 gtcacaatca gatggtgcag agaaggcgcg tgaagcaatt gggcaagaag cgcctgatac
     2341 aggcagccaa gcaggccaag aagctgagaa aacaaaacgc caggatgaaa tctcaggctg
     2401 cgtgatagag agtttcctgg gcgccgcccg tcagggaacg acgggtagcg cggcgccggt
     2461 cttccccttg agatactccg ctttcagctt ctgaatggcg gccaggtact cttccatgct
     2521 ctaacgaccg cggaagacag ctccaagaaa aaagccccgc gcaggcgggg cctttttgtt
     2581 tcttgggaaa ccgggggcag accgtggttt ccggtcaatc aagccccggt cttgatacgg
     2641 gcttcaagcc ccgcgacaca ggcactggat acgccccaag cagccttttc ttccccgggc
     2701 atcttgtccc agccgccttt ttcaatgagc tcggatctct ggtagctttc cgttccctga
     2761 aattccttga tattgatctt gtggttcggg ctgttcatgt attgggcgac gcagatcgct
     2821 accttgctcg tcagcaccgc ctcttcgctc atcttctggg tggtgctgga ggtcgtccag
     2881 ccgccccaca cgaagccgat cgtcattgca acgccggccc cgagaacaaa cccccaaatt
     2941 ccaaacttta ccttcgcatc cgtttctggc gtgatcgtca ttggtcatac ctctcaaagt
     3001 gtggcggtct cgctaagccc cctgcatttc ccgctgtttc gcctatccag gatttgcgtg
     3061 ccatggctcc cgcgctatcc ggtgcgggaa ctgggacttg ggctaccgta ccgtaattgt
     3121 ctgcgctgcc cgagacgcgg tctgtgcgct accgaacata ggcttaccag atgggtgatg
     3181 agtctcccgc gcagcggggg agattcgtca ctgcgtcact tcgtcactgc atcttagccc
     3241 gccgcggcat gaccagatta ccgacgtaga cctgcaccgg ctcgccactt gaaggatgaa
     3301 ggcagaagga tgaaggagga agttccccgc gtgttgaacg gtggcgcctc gtcctttcga
     3361 acacgcggag ttaaaccggc gcaaagcgtc tggttttaac aatacttggc gttgtcttgg
     3421 cgtcctttcg catgccgtga ccagtgacgg gtcttaagcc cgtgagatcg ggagattgaa
     3481 aaaccgggag gtcgtgaaat cgggagatcg tgagatcaaa accgagcttg tcgcactccc
     3541 gtcctgacga tagttgtcgc tctcccgctc tcccgccctt acgccctccc gtaccctgtc
     3601 ttctgccgct ggcctgtccc ggagggcaga ttgatttacg tccggagttc cgctttcagc
     3661 gcatccggcg taaggatcgg tacgcgcgac ctgccggcga gcgcgagcaa atcctcgtcg
     3721 cccgtcacca gagcgtccgc tttggccgca taggccaatt gcaggaactt ccggtcatcc
     3781 ggatcgcggc attcggggac ccgaacggag gcgccgccct cgcgcacggc ctcggcgtgc
     3841 tccatgtagt gcgcgagcac gttcttcgta tcctcctcgg aaagcttgag cttcgggtag
     3901 gcaagcacgc gtaccagttc ggcgagcgtt tccgcggaaa cgagggggac gattcggccc
     3961 gcctcccacg cctcccgcag ccaggcaagc cggccctcgc ggaacaccag ggccgagact
     4021 gcgacgttgg tgtcgagcac gacgcgccgc gggcgggcta cttccgcttt ctcgcccacc
     4081 gcaccgcgcc ttcgacgtcc ttctcggtga tgccgagcga ctcgatcttg cgccagacgc
     4141 cctcgaggct cgtcaccttc gccggcacca gcacgatgcg gcccttttcg gcgcgcacct
     4201 cgaagtagcg cgcgcccggg aattggccga ccaccgcctt gggcagggtg agctggttct
     4261 tggaggtcaa cttggccagc atgactttat tccttttagt aaggaatcct tattatacgc
     4321 ttttttgttc cccgctggtg cgtcaggact ccttgttctg gatgctgaac cgcaaatttc
     4381 aagctgcaag tttcaagctg gaccagcggt acttcgccct cgtaacccgg aacttgaaac
     4441 tggtagttga cggctgacaa gtgacgagta acgagcgacg agtgacggcc tcgatccgcg
     4501 ctactgatcg gcgagctcca gcagcgcggc ggtgagcgcg cgcgcgccgg cggcgaggtc
     4561 ctcgggcctg gcgttctcgg cctcgttgtg gctgattccc cgctcgcacg gcacgaagat
     4621 catgccggtc gggcacacct gcgccatgta catcgcgtcg tgactcgcgc cggagggcat
     4681 gcgctggtgc gcgagcttca gcgcctgcgc cgcgcgctcc acgcagtcca ccaccgcggg
     4741 atcgaacgcg cacggatcgt cgtggagcgt gggcgtgacc ttgaccgagc agcgcgtcac
     4801 ggccttccgc accgccggct cgaccgcttc gcccaggcgc gcgatcgtcg ccgcgtcggg
     4861 atggcgcacg tccaccgaga acatgacgtg gctcggcacc gaattgggcg agttcggcgt
     4921 gaccagcacg cgcccgatcg tgaagcgcaa cgtgtcgctc gcatcgcgcg tgagctcgtg
     4981 cagcgcgttg atcgcggcga tcgcctcgcg cagcgcgtcc ttgcggccgg cgagcggtgt
     5041 cgtgccggcg tgcgcggtct cgccgaagac ctcgacgttg aaccagcgca agccctggat
     5101 gccggtcacc acgccgatgg tcctgccttc ctgctcgagc agcggcccct gctcgatgtg
     5161 cgcctcgatg taagcggcgg cgggcgcgtt cagctcgcgc tgttcggccg cgggcgtggc
     5221 ggcgagcgtc tgcgccagcg catcctcgag cgcgacgccc tcgttgtcct tcacctcgag
     5281 aaaatcgccg agcggcttcg ccccggtgta caccgctgaa cccatggtgc agggctgaaa
     5341 gcgcccgcct tcctcattgc tccacgcgac gagttcgacc gggtggcggg tcttaacccc
     5401 agcctgttct atcgcttcga gcgcttcgag gccagcaagc accccgtaga tcccgtcgaa
     5461 acgcccgccg cgcggctggc tgtccatgtg gctgccggtc atcaccggcg ctgcgcgcgg
     5521 atcggtcccg gcgcggcgga tgaaaaggtt gccgatcgcg tccatcgcca cggcgaagtt
     5581 gcgcgcgcgc gcccaggaga tcagcagctt ccgggctgcg atatcctcct tcgaaaacgc
     5641 cgcgcggttg acgccgccgc cgggaatggc gccgatctgc gccatctcca tgaggcgccg
     5701 ccacagccgc tgttgatcga cgcggttgac ggcgtcctga tgcgcttctc tcatgcgact
     5761 ggcggcgatg agccgtgccg ggctactcga tgcgtgcgcc ggatcgcctc actacccggc
     5821 cccattttt
//
LOCUS       DBNGWZ010000026        16577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2502, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000026 DBNGWZ010000000
VERSION     DBNGWZ010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2502"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16CX_00685"
     CDS             <1..311
                     /locus_tag="AC16CX_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3011058.1"
                     /translation="LEGMLKAKWLVPEGRDFRVTRLGEKELAAFGVDLDTARRSRRVL
                     ARACVDLTQRRPHLSGALAAALLDLYTKRGWILRARRSRVVTITPKGQEAFRRMFGAT
                     "
     gene            complement(308..1483)
                     /gene="argE"
                     /locus_tag="AC16CX_00690"
     CDS             complement(308..1483)
                     /gene="argE"
                     /locus_tag="AC16CX_00690"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HIR3011059.1"
                     /translation="MVSGSASLFPSVEMLRKLVAFPTVSRDSNLDLIHFIRDTLAPYD
                     AAPRLTYDDGKRKANLFATLGPRGEPGIALSGHTDVVPTEGQAWDTDPFEMVEKDGRL
                     YGRGTADMKGFIACVLALAPEFVERGLKTPLHLAFSYDEEVGCLGVGRLIADLMRAGL
                     RPSACIVGEPTEMRPVVAHKGKKSYRCTVRGLAGHSAYAPHGVNAVEYAAEAIAFLKN
                     LARRHRDQGPYDRGFDVAHTTVHTGVIRGGTALNVIPHECTFDFEFRHLPGDDPERLL
                     HEFRDYVKSRLEPEMRAVHAAAGFVIEPLSVIPMLDTGTESEVVALAHKLSGSGEIGK
                     VSFATEASQFHRAGIPTVVCGPGSIAQAHKPNEYVTLEQIARCGEFLHRLMERMCKA"
     gene            1813..3366
                     /locus_tag="AC16CX_00695"
     CDS             1813..3366
                     /locus_tag="AC16CX_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011060.1"
                     /translation="MKALFALLLLLFPLAAPAADLSIALGGDVTSIDPHFHNLTPNNN
                     IGAHVFDTLVAKNAAGKIRPALAESWRAVDDLTWEFKLRRGVKFHDGSEFTAADVVFS
                     LDRIPNVPNSPSPFTTYTKQVTEKIVVDPWTIRLKSTAPYPQMPNDMSTVLVVSSRAA
                     ANATTEDFNSGKAAIGTGPFKFVRWQKGDRIELARNDNYWGPKAPWDKVTFRIITSDP
                     TRVASLLAGEVRAIENVPTNDLAKLAANRDLNLYRTVSVRLMYLHLDTAREKTPFAAD
                     KTGKLLEKNPLRDLRVRRAMSKAINRQALVERIMEGAAVATGQLMPEGFFGYAPALKP
                     EAYDPEGAKKLLAEAGYPDGFGLTLHAPNNRYVNDEQIAQAVAQMLAKVGIQTRVDAM
                     PSSVYFSRGSRLEFSFLLVGWGAETMEASSPLKALLATFNKDKGMGAANRGRYSNPRL
                     DAVLEQALATVDDAKREKLLQQATEIGVGDLGIIPLYHQVNLWAARRGIVYEARADER
                     TFAHEFRPQ"
     gene            3426..3791
                     /locus_tag="AC16CX_00700"
     CDS             3426..3791
                     /locus_tag="AC16CX_00700"
                     /inference="COORDINATES: protein motif:HMM:NF016370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HIR3011061.1"
                     /translation="MPRLSTKTVIISRRLRGNLTDAEKLLWRHLRLRQMGGFRFRRQH
                     PVANYIVDFACIEARVVVEVDGGQHTTQPVQDSRRTMILERRGYRVLRFWDNEVLGDV
                     EAVKAVIWSVLREKKPPPP"
     gene            3752..5362
                     /locus_tag="AC16CX_00705"
     CDS             3752..5362
                     /locus_tag="AC16CX_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011062.1"
                     /translation="MVSIERKETTPTLTLPLRGRGLKWVLPLLLSLATATVVGAAELK
                     IGLSADVTTFDPHFVAAQPNLTAQHHVFEPLAHVDERGRPIPVLATWRTPDSLTWEFT
                     LRKGVKFHDGSELTTEDIVFSLERPYTIIGSPGGFTVYVRPIVAKEIVDRYTIRLKTA
                     APYGGVVQDLAEVMIVSKKVAQGAGSADFDGGKAAIGTGPYKLVRFARGNRIELVRNE
                     TYWGGKLPWDKVTLIIVPSDPVRTASLLSGELDAIEHVPTADLARLKKNPAFRLEQAV
                     SWRTIFLHVDQSRDRPPGVLSKTGKPLERNPLKDLRVRRALSKAINRQAIAERVMEGL
                     ALPAANVVSPSVFGHNPAVKPEPYDPEGAKKLLAEAGYPDGFAVTVATPNNRYINDEQ
                     VAQTVAQMFARIGIAAKVEAMPLSVYFGKARNREFGIALLGWGSLAADLALRSLAATA
                     NPDKGYGAWNWGGYSNPKLDQLIERSLGTVDPVKREAAAREAAALAAAEVTFIPLHYQ
                     VVTWAMKNNLSYTARTDEFTFAHHFQQR"
     gene            5474..6451
                     /locus_tag="AC16CX_00710"
     CDS             5474..6451
                     /locus_tag="AC16CX_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011063.1"
                     /translation="MISFALRRLLQSVLVLFVMSALVFAGVYAIGNPVDILINPQADQ
                     AEIARATAALGLDQPLWRQYWNFIVGAFAGDLGKSFAANIPAVHLIFERMPATLELAL
                     AATVIAVTLGIPLGLWAGLRPDSAAGRLIMAGSILGFSLPTFWVGLMLIMVFSVSLGW
                     LPPTGRGATVMVLGVPVSFLTWDGLLHLFMPALNLALFKLSLLIRLTRAGTREALAQD
                     YVRFARAKGLSMERVVRVHVLKNIMIPIVTVTGLEFGSVIAFAIVTETIFAWPGMGKL
                     LIDSINVLDRPVIVAYLLIIVLLFVLINLVVDLAYTALDPRIRLAGRTA"
     gene            6657..7565
                     /locus_tag="AC16CX_00715"
     CDS             6657..7565
                     /locus_tag="AC16CX_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011064.1"
                     /translation="MNQQVIRHPSALIPQVSRDFAQDRIAVAALAVFSILLLAAVGAP
                     LVSPQDPYNLAQLDILDSKLAPGSKAGAGFTYWLGSDEQGRDMFSAILYGLRISLGVG
                     ATSTLVALAIGMAVGLAAAYAGGWLDNLIMRLVDLQLSFPAILIALVLLAVTGQGVDK
                     IVIALILVQWAYYARTVRGAALAEKKREYVDAARCMAFSAPRIVFRHILPNVLPPLIV
                     VATVQIAHAIALEATLSFLGLGMPITSPSLGLLISNGFAHLLSGRYWISFFPGVALLI
                     TIVAINLVGDRLRDVLNPRLGSHYGV"
     gene            7729..10881
                     /gene="putA"
                     /locus_tag="AC16CX_00720"
     CDS             7729..10881
                     /gene="putA"
                     /locus_tag="AC16CX_00720"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006562 - L-proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HIR3011065.1"
                     /translation="MIITAHPTSEAASLGPVAEAFLKDEPAAVKELLATAAMDAGMKR
                     GAQRRATRIVSAIRSRGGPEGLLAPLLREFDLASEEGLVLMCLAEALLRIPDPETVDD
                     LIREKLTGPEWEKHLGASSSLQINASVLGLMLADWVVAGGVDQARDFTDIFLRLLARS
                     GEPLVRRAVQHGMQLIGRQFILGETIGEALERAAEMESRGVRFSYDMLGEAAVTAGDA
                     ERYFARYLDAIRDVGAAAARGAFTDLHSRPGVSVKLSALHPRYAAPQRRRVLDELVPR
                     LRNLAREAADRGISLCVDAEEADRLELSLEVIEAVATDPGIRDWQGLGLAVQAYQKRA
                     PHVIDWLAEAALRLQRRWMVRLVKGAYWDTEIKRAQERGLSEFPVFTRKAHTDVSYLA
                     CARRLLSAPECFYAQFATHNALTAASIAEMLPPGSAYEFQRLHGMGEHLYETLHGEFP
                     ATRYRVYAPVGSHRELLPYLVRRLLENGANNSFVNGLADPAVPVEMLVADPLEKAAAS
                     GGARSARIALPRDLFLPERVNSQGFDLSDRLALGELARAMSDLDGRHWDAAPLVPGRA
                     PGAPPEPVYCPARRSRVIGEVRPATAGDVEAAMKTAMGAFSSWTSRRVDERARLLEQA
                     AGALERGRAEFIHLLIHEGGKTIPDALAEVREAADCCRYYAAQARRDFGAPVALPGPT
                     GEQNQLSLHGRGVFACISPWNFPLAIFLGQITAALAAGNTVIAKPAEQTPLIAAAAVR
                     LLHEIGIPAEVLCLLPGPGAEIGARLVADQRLAGVAFTGSTDTAWRINAALAARRAPI
                     APLIAETGGQNAMIVDSSALLEQAAMDIVTSAFDSAGQRCSALRVLLVQDEVAPRLIE
                     MLRGAMAELAVGDPARLDADVGPVIDEEALGALENHVAAMARAGRNVFRLPLPADCAG
                     GTFCAPTLIEITGTALPDHEVFGPILHVARYQRDRLEQVLETVRDTGYGLTLGIHTRL
                     AGRAAEIARSLPVGNVYVNRNMIGAVVGAQPFGGQGLSGTGPKAGGPHYLQRFAVEKT
                     TTINTMAAGGNAALLAGEDC"
     gene            11145..11825
                     /locus_tag="AC16CX_00725"
     CDS             11145..11825
                     /locus_tag="AC16CX_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011066.1"
                     /translation="MQIYKTPSKPAFMNSFSCSLIFLLMLVTHATSNAEERTTQSRTD
                     SDSKLQPYIRCDGFAGGVRAVILDRRPQTAEPWRAADLGGSSQRVSVVDGYRVIYSYV
                     RTYAFANLKAERSDPSRYPEDKRIVISELVEMAKTDGAVDLVDFSHGGFSGQMLTKRA
                     LGGRALAKSQIFSDEDSIIVSIFFLNHAPENRKFRSYDEFISLRDAFIRGYIDCVAKK
                     KSGAIRSQ"
     gene            12038..12757
                     /locus_tag="AC16CX_00730"
     CDS             12038..12757
                     /locus_tag="AC16CX_00730"
                     /inference="COORDINATES: protein motif:HMM:NF037889.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR3011067.1"
                     /translation="MPTARPRGRPPKAKHLDTLLANLEEVTRLMEIHTKVAGRTVGAK
                     HKVEVLNKSGIVLLVACWEAFVEDLAGVSFRLLLRRAKRPDVFPSKVLALASKNLRED
                     RDERRVWDLAGDGWKTVLRSHQENVVKRYIGNLNTPKPNQIDELFASVIALGKVSKKW
                     SWHRCSPAKARARLIKLVTLRGAIAHRVSTPRSVLKRDVERAAKFVGRLAVATHNAVN
                     TSLRVRLGHSPWQDYVYRDAG"
     gene            12857..14386
                     /locus_tag="AC16CX_00735"
     CDS             12857..14386
                     /locus_tag="AC16CX_00735"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3011068.1"
                     /translation="MSKPTAGHLLWTALILAFFFIIAALVEYKFVGWQVRNAALSDLR
                     DEAQQLNNAIAFDKGINLEQFNRAFVDAGDYFVVLSDGAIIDIASGSKGMLRGVLPNV
                     QPAFSVEDALRAPLTIDHSVGGKAESWTVLAKKLEGGVAILGVSAFDAMPAAADILQR
                     NIQRIGRRLSDVVHLNPGVLDNSLHWAVISDAGELITAFGRLPLKTDPMAIGKQSTSN
                     PEVMIEGKPYLVLYAPLLDKSGQPAGTVILPQSLEREKEVLWKELLFDASVSATSFAV
                     FLILAAWYFSKHEREKRAIREQFQKFLPPKIQDEVLNDPSQAALGGLRREITILFSDI
                     RSFTTLCERLPPQQLTRLLQEYFEAMTEEVMKEEGYVDKYIGDGIMAFWGAPIDQKDQ
                     ADRAVRTAQAMIRRLRELQDQWRAAGYPIFDIGIGINLGVATVGNMGSSKHFDYTAVG
                     DTVNAASRLEQLTKTYANHIIISESTKNQLEIRPPLKDLGEVKVIGKENPVHIYEVLP
                     D"
     gene            14660..14833
                     /locus_tag="AC16CX_00740"
     CDS             14660..14833
                     /locus_tag="AC16CX_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011069.1"
                     /translation="MVVAAQVFIPIWLAAAGVNMWVGVAKAGYSVAEELPIFLLIFAL
                     PAAAAAFVWWKFS"
     gene            14918..15271
                     /locus_tag="AC16CX_00745"
     CDS             14918..15271
                     /locus_tag="AC16CX_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011070.1"
                     /translation="MKRSAWIALILLAGMQQAVFAAEARLNEQQKLGRRLFEQSCGVC
                     HTRPTLVSGMYGPELSRESAGGREDLMREIIRSGTARMPGFKYTYNPAQIAAITAYLK
                     TVPPGTQDLPTVRKH"
     gene            15331..>16577
                     /locus_tag="AC16CX_00750"
     CDS             15331..>16577
                     /locus_tag="AC16CX_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3011071.1"
                     /translation="MKSPRSFTGASLIALAAALGSATLVPGAAHADALLTGAVTSAAG
                     EKMGGVTVSAKAEGSPITTSVFTDETGSFYFPPLPDGKYRVWAQALTYRTAKGAVELR
                     KTTRRDFVLRPMKNREDWIRQLPGDEFLAALPGDTPEDYRMKTQVRKNCTGCHSASYP
                     LQHRFDEEGWRKVLDLMKHVNVLGVYFGPDHKATPNIEFHQKELTAYLARARGPGASS
                     MKFKLRPRPSGEAARAVIREYDFPMEGGHARSMDGSDWSLGTPSNMNHVAGVHDAQMD
                     FDGNIWITFSHTSTETTIARIDGKTGAVKHFRLDDQRGIAIGSHGITRDENGILWFNT
                     RSHVERIHGSLGRIDPRTEKITVYSPPKPMSGTSGTLDADMNGNVWVTSPDGALRFNI
                     KEEKFTEFKSVTYKNQHGTATVYG"
BASE COUNT         3186 a         5220 c         5178 g         2993 t
ORIGIN      
        1 tcctcgaggg gatgctgaaa gcgaaatggc tggtccccga ggggcgggat ttccgtgtca
       61 cccggctcgg cgagaaggag ctggcggcgt tcggcgtgga cctcgacacc gcgcgccggt
      121 cgcggcgtgt gctcgcgcgc gcctgcgtgg acttgaccca gcggcgtcct catctcagcg
      181 gcgcgctggc ggccgcgctg cttgatcttt ataccaagcg gggctggatc ctgcgcgcgc
      241 gccgctcgcg cgtcgtcacg atcacgccca aaggacagga ggccttccgg cggatgttcg
      301 gagcgactta agccttacac atccgctcca tcagccggtg taggaactct ccgcaacgcg
      361 cgatctgctc cagcgtgacg tactcgttgg gcttgtgcgc ctgcgcgatc gagccggggc
      421 cgcataccac cgtcgggatg ccggcgcggt gaaactgcga ggcctcggtg gcgaacgaga
      481 ccttgccgat ctcgccgctg ccggacagct tgtgggcgag cgccaccacc tcgctctccg
      541 tcccggtatc gagcatcggt atcacggaaa ggggctcgat gacgaagccg gcggcggcgt
      601 ggaccgcccg catctccggc tccagccggc ttttcacgta atccctgaac tcgtgcagca
      661 gccgctcggg atcgtcgccc ggcaggtggc ggaactcgaa atcgaacgtg cactcgtgcg
      721 gaatgacgtt caacgccgtg ccgccgcgga tcacgccggt gtgcacggtg gtgtgcgcca
      781 cgtcgaagcc gcggtcgtag ggtccctggt cgcggtgccg gcgcgcgagg ttcttgagga
      841 acgcgatcgc ctcggctgcg tattcgacgg cgttgacgcc gtgcggcgcg tacgccgagt
      901 gcccggcgag gccgcgcacc gtgcagcggt agctcttctt gcccttgtgg gcgaccaccg
      961 ggcgcatctc ggtcggctcg ccgacgatgc atgcgctcgg gcgcaagccc gcgcgcataa
     1021 gatcggcaat caatcgtccg acgccaagac agccgacttc ttcgtcatag gaaaatgcga
     1081 ggtggagcgg cgtcttcagg ccccgctcca cgaattccgg cgccagcgcg agcacgcagg
     1141 cgatgaagcc tttcatgtcg gcggtgccgc ggccgtagag cctgccgtcc ttctcgacca
     1201 tttcgaaggg atcggtgtcc cacgcctgac cctcggtcgg cacaacgtcg gtatggccgg
     1261 acagcgcgat gcccggctcg ccgcgcgggc cgagcgtcgc gaacaggttg gccttgcgtt
     1321 tcccgtcgtc gtaggtgagc cgcggcgccg cgtcgtacgg cgccagggtg tcgcggatga
     1381 aatgaatcag gtcgaggttc gaatcgcggc tcaccgtggg aaacgcgacg agcttgcgca
     1441 gcatttccac gctcggaaac aggctcgcgg aaccggaaac catgatcgct cccgcgctcg
     1501 ggaaaggatc gagcataggt agcgcggtga gtcgtgtcaa acccggtttc gccagcagcc
     1561 tgtcggactt aatagtcagt acaggctgct aacgacaaga ccggcctgac aattcagaaa
     1621 aatcccagcc gaggcggtcg aatgtgaaga atagcgagag tgaattcgca tacctgtcat
     1681 cgccttttgc ttttttcctg gcaccggtct tgcattagga gtttgtcggg gctaagtccg
     1741 gcaaactcct gggcgggcgg cgcgcgaagt acgctatgct tcaccgcgtc ccgcgcgacg
     1801 aaggaagata ccatgaaagc gctgttcgcc ctcctgctgc tcctcttccc gctcgccgcc
     1861 cccgccgccg atttgagcat cgccctcggc ggggacgtga cgtccatcga tccgcacttc
     1921 cacaacctca cgccgaacaa caacatcggc gcgcacgtgt tcgacacgct cgtcgccaag
     1981 aacgctgccg ggaagatcag gcccgctctc gccgagtcct ggcgcgcggt ggacgatctc
     2041 acctgggagt tcaagctgcg caggggcgtg aagttccacg acggctcgga gttcaccgcg
     2101 gccgacgtgg tgttctcgct cgaccggatc ccgaacgtgc cgaacagccc gtcgccgttc
     2161 accacctaca ccaagcaggt gaccgagaaa atcgtggtgg atccgtggac gatccggctg
     2221 aagagcaccg cgccgtaccc gcagatgccc aatgacatga gcacggtgct cgtcgtgtcc
     2281 tcccgggctg ccgccaacgc gacgaccgag gacttcaact ccggaaaggc ggcgatcggc
     2341 accggtccct tcaagttcgt gcgctggcag aaaggcgatc gcatcgagct cgcgcgcaac
     2401 gacaactact ggggcccgaa ggcgccgtgg gacaaggtga cgttccgcat catcacctcc
     2461 gacccgacgc gggtggcgtc gctgctcgcg ggggaggtgc gcgccatcga gaacgttccc
     2521 accaacgatc tggccaagct cgccgccaac agggacctca atctctaccg cacggtctcg
     2581 gtgcggctca tgtacctcca cctcgacacc gcgcgcgaga aaacgccgtt cgcggccgac
     2641 aagacgggca agctgctcga gaagaacccc ctcagggacc tgcgcgtgcg gcgcgcgatg
     2701 tccaaggcga tcaaccgcca ggcgctggtc gagcgcatca tggaaggcgc ggcggtcgcg
     2761 accggccagc tcatgccgga aggctttttc ggctacgcgc cggcgctgaa acccgaggcg
     2821 tacgatccgg agggcgcgaa gaagcttctc gccgaagccg gttaccccga cggcttcggg
     2881 ctgacgctgc acgccccgaa caaccgctac gtcaacgacg agcagatcgc gcaggcggtc
     2941 gcgcagatgc tggccaaggt cggcatccag acgcgcgtgg acgcgatgcc gtcctcggtg
     3001 tacttctcgc gcggctccag gctcgagttc agcttcctgc tggtcggctg gggcgcggaa
     3061 accatggaag cgtcctcgcc gctgaaggcg ctgctcgcga ccttcaacaa ggacaagggc
     3121 atgggtgcgg cgaaccgcgg ccgttattcg aatcccaggc tcgacgcggt gctggagcag
     3181 gcgctcgcca cggtggacga cgcgaagcgc gagaagctgc tgcaacaggc caccgagatc
     3241 ggcgtcggcg accttggcat catcccgctc taccaccagg tcaacctctg ggcggcgcgc
     3301 cgcggcatcg tctatgaggc gcgcgcggac gagcgcacct tcgcgcacga attccggcct
     3361 cagtgacccc ttatttctcc ttccccctca gggggaaggt tgggatgggg gtggttcaca
     3421 gcagtatgcc tagactcagc accaagactg taatcatttc gcgtcgccta cgtggcaacc
     3481 ttacggacgc tgaaaagcta ctctggcggc acctacgttt gcggcagatg ggaggtttcc
     3541 gtttccgtag gcagcatcct gttgctaact acattgttga cttcgcttgc atcgaagcac
     3601 gtgtcgtagt cgaggttgac gggggtcagc atacaaccca acctgtgcaa gattcacgaa
     3661 ggaccatgat tttggagcgc aggggctatc gagttctacg attttgggac aacgaagtgc
     3721 ttggagatgt tgaagcagtt aaagcggtga tatggtcagt attgagagaa aagaaaccac
     3781 ccccacccta accctccccc tgagagggag gggattaaag tgggtactgc ctctgctgtt
     3841 gagcctcgcc acagcaacgg ttgttggtgc cgctgagttg aagatcggct tgtccgccga
     3901 cgtcacgacg ttcgatcccc acttcgtcgc cgcgcagccc aacttgaccg cccagcacca
     3961 tgtgttcgag ccgctggcgc acgtggacga gcgtggccgg ccgatcccgg tgctcgcgac
     4021 ctggcgcacg cccgattccc tgacctggga gttcacgctg cgcaagggcg tgaagttcca
     4081 cgacggctcc gagctgacga ccgaggacat cgtgttctcg ctcgagcggc cgtacaccat
     4141 catcggcagc ccgggcggct tcaccgtcta cgtgcgtccg atcgtcgcca aggaaatcgt
     4201 ggaccgctac accatccgcc tcaagaccgc ggcgccgtac ggcggcgtgg tgcaggacct
     4261 cgccgaagtg atgatcgtgt cgaagaaggt ggcgcagggc gcaggcagcg ccgacttcga
     4321 cggcgggaaa gccgcgatcg gcacgggacc gtacaagttg gtgcgcttcg cccgcggcaa
     4381 ccggatcgag ctcgtgcgga acgaaacgta ctggggcggc aagctgccgt gggacaaggt
     4441 gacgctcatc atcgtgccga gcgatccggt gcgcaccgct tccctgcttt ccggcgaact
     4501 cgacgcgatc gagcacgtgc cgaccgctga cctcgcgcgg ctgaagaaga accccgcgtt
     4561 ccggctcgag caggcggtct cgtggcggac catcttcctg cacgtggacc agtcccgcga
     4621 ccggccgccc ggcgtcctgt ccaagaccgg caagccgctc gagcgcaatc ccctgaagga
     4681 tctgcgcgtc cgacgcgcgc tgtcgaaggc gatcaaccgg caggcgatcg ccgagcgcgt
     4741 catggagggt ctcgcgctgc ccgccgcgaa cgtcgtctcg ccgagcgtgt tcggccacaa
     4801 ccccgccgtg aagcccgagc cgtacgatcc cgagggcgcg aagaagctgc tcgcggaagc
     4861 cggctatccc gatggtttcg cggtgacggt cgccacgccc aacaaccgct acatcaacga
     4921 cgagcaggtc gcgcagacgg tggcgcagat gttcgcccgc atcgggatcg cggcgaaagt
     4981 ggaagccatg ccgttgagcg tctatttcgg caaggcgcgc aaccgggagt tcggcatcgc
     5041 gctgctcggc tggggctcgc tcgccgccga tctcgcgttg cgctcgctcg ccgccaccgc
     5101 caatccggac aagggctacg gcgcgtggaa ctggggcggc tacagcaacc cgaagctcga
     5161 ccagctgatc gagcgatccc tcggtaccgt agacccggtg aagcgcgaag ccgccgcgcg
     5221 cgaggccgcc gcactggcgg cggccgaggt cactttcatc cccctgcact accaggtcgt
     5281 gacctgggcc atgaaaaaca atctttctta cacggcccgg acggatgaat tcaccttcgc
     5341 gcatcatttc cagcagcggt aacggaaagg cggggctagg gactggggac taggggctag
     5401 taagatcaaa tacgtcaaaa ccgagccccg agtctcgagc ctcgagtccc gtattcctcc
     5461 acgatcattc cgcatgatct cgtttgccct ccgccgcctc ctgcaaagcg tgctggtgct
     5521 gttcgtgatg tcggcgctgg tgttcgcggg cgtgtacgcg atcggcaacc ccgttgacat
     5581 cctgatcaac cctcaagccg accaggccga gatcgcgcgc gcgaccgccg cgctcgggct
     5641 cgaccagccg ctctggcgcc agtactggaa cttcatcgtc ggcgcgttcg cgggcgatct
     5701 cggcaagtcg ttcgccgcca atatcccggc ggtgcatctc atcttcgagc gcatgccggc
     5761 gacgctcgag ctggcgctcg cggccacggt catcgcggtc acgctcggca tcccgctcgg
     5821 gttgtgggcc gggctcaggc ccgattcggc cgcggggcgg ctcatcatgg cgggctccat
     5881 cctcggcttc tcgctcccca ccttctgggt cgggctgatg ttgatcatgg tgttctcggt
     5941 gtccctcgga tggctgccgc ccaccgggcg cggtgcaacc gtgatggtgc ttggcgtgcc
     6001 ggtgagcttc ctgacctggg acgggttgct gcacctcttc atgccggcgc tcaatctcgc
     6061 gctgttcaag ctctcgctgc tgatccgtct cacgcgcgcg ggcacgcgcg aggcgctggc
     6121 gcaggactac gtgcgcttcg cccgcgccaa gggcctctcg atggagcgcg tggtgcgggt
     6181 gcacgtgctg aagaacatca tgatcccgat cgtgacggtt acggggctgg agttcggctc
     6241 ggtgatcgcg ttcgccatcg tcaccgaaac catcttcgcg tggccgggca tgggcaagct
     6301 cctcattgat tcgatcaacg tgctcgatcg gccggtgatc gtggcctatt tgctgatcat
     6361 tgtgctgctg ttcgtgctga tcaacctcgt cgtggatctt gcgtacacgg cactggatcc
     6421 gcgcatccgc ctcgcggggc gaacggcgtg aacaaaacga accgccgaga gaagcaaaac
     6481 aatattgaac cgccaagacg ccatgagaac caaaatagaa atcgaaccac agagattggg
     6541 agagaaacaa aaataaaatc gaaccgccaa gcgcgccgag agaatcgaaa acaaattcga
     6601 accgcagaga tggagagaga agcaaagata atcatgaaca accaagacga aaagacatga
     6661 accagcaggt catccgtcat ccctccgccc tcatccctca ggtctcgcga gatttcgcgc
     6721 aggaccggat cgcggtggcg gcacttgcag tgttttccat tttgctgctg gccgcggtcg
     6781 gtgccccgct cgtctcgcca caagacccat ataacctggc ccagctcgat attctcgact
     6841 cgaagctcgc accgggatca aaagcaggcg cgggattcac ctattggctc ggcagcgacg
     6901 agcagggccg cgatatgttc tccgccatcc tctatgggct gcggatcagc ctcggggtcg
     6961 gcgccacctc cacgctcgtc gcgctcgcga tcggcatggc ggtcgggctc gccgccgcct
     7021 acgccggcgg ctggctcgac aatctcatca tgcggctggt ggacctccag ctctcgttcc
     7081 ccgccatcct catcgccctg gtgctgctcg cggtgacggg acagggcgtg gacaagatcg
     7141 tgatcgcgct gatcctcgtg caatgggctt actatgcgcg cacggtgcgc ggcgcggcgc
     7201 tggcggagaa gaaaagggag tacgtggacg ccgcgcgctg catggcgttc agcgccccgc
     7261 gcatcgtttt ccggcacatc ctgccgaacg tgctgccgcc gttgatcgtg gtggcgacgg
     7321 tgcagatcgc gcacgcgatc gcgctcgagg ccaccctctc cttcctcggg ctcggcatgc
     7381 cgatcacctc gccctcgctc ggcctgctga tctccaacgg gttcgcccac ctgctgtcgg
     7441 ggcgctactg gatcagcttc ttccccggag tggctctgct cattaccatc gttgcaatca
     7501 atttggtagg ggatcggcta cgcgacgtgc tcaatccccg gctcggcagc cactacggag
     7561 tctgaaccgc caagagaacc ttccaataat tggaccgcag agatggagag agaaacaata
     7621 acaaaattga accgccaagg cgccaagaga aacaagattt gaataaaata gtgcgcgtcc
     7681 cctcttcatc catttggcgc ttccagaggg gtatccgcga gggcgcgcat gatcatcaca
     7741 gcccacccta ccagcgaggc cgcaagtctc gggccggtcg cggaagcgtt cctgaaggac
     7801 gagccggccg cggtgaagga gctgcttgcg acggccgcga tggatgccgg catgaagcgg
     7861 ggcgcccaac gccgggccac tcgcatcgtc tcggcgatac gctcgcgcgg cggcccggag
     7921 ggtctgctcg cgccgctgct gcgcgaattc gacctcgcca gcgaggaggg gctcgtcctg
     7981 atgtgcctcg ccgaggcgct gctgcgcatc cccgacccgg agaccgtgga cgacctcatc
     8041 cgcgagaagc tcaccggccc ggagtgggaa aaacatctcg gcgccagctc ctcgctccag
     8101 atcaacgcct cggtgctcgg cctgatgctc gccgattggg tggtggcggg cggcgtggat
     8161 caggctcgcg acttcacgga tatcttcctc cggttgctcg cgcgcagcgg cgagcccttg
     8221 gtgcgccgag ccgtccagca cggcatgcag ctgatcggtc gccagttcat cctgggcgag
     8281 accatcgggg aggcgctcga gcgcgcggcg gagatggaaa gccggggcgt ccgcttctcc
     8341 tacgacatgc tgggagaggc cgcggtcacc gctggcgatg ccgaacgcta ctttgcgcgc
     8401 tatctcgacg cgatccgcga cgtcggcgct gccgccgcgc gcggggcgtt caccgatctg
     8461 cattcgcgtc ctggcgtgtc cgtcaagctc tcggcgctgc acccgcgcta cgcggccccg
     8521 cagcgcaggc gcgtgctcga cgagctcgtt ccgcggctgc gcaacctcgc gcgcgaggcc
     8581 gcggaccgcg gcatctcgct ctgcgtggac gcggaggaag cggaccggct cgagctctcg
     8641 ctcgaagtga tcgaggcggt cgcgaccgat cccggcatcc gcgactggca gggcctggga
     8701 ctggccgtgc aggcatacca gaagcgcgcc ccgcacgtga tcgactggct tgccgaggcg
     8761 gcgctacggc ttcagcgcag gtggatggta aggctcgtca agggcgccta ctgggacacc
     8821 gagatcaagc gcgcgcagga gcgcgggctg agcgagttcc cggtcttcac gcgcaaggcc
     8881 cacaccgatg tctcctacct ggcctgcgcg cggcggctgc tttccgctcc cgaatgcttc
     8941 tatgcgcagt tcgcgacgca caatgcgctc accgcagcgt cgatcgccga gatgctgccg
     9001 ccgggaagcg cctacgagtt ccagcgcctg cacggcatgg gcgagcacct gtacgagacg
     9061 ctacatggag aatttccggc gacgcgctac cgcgtctacg cgcccgtcgg cagccaccgc
     9121 gagctgctgc cttacctcgt gcgccggctg ctcgagaacg gcgccaacaa ttccttcgta
     9181 aacggtcttg ccgacccggc ggtgccggtc gaaatgctcg tcgccgatcc cctcgagaag
     9241 gcggccgcga gcggcggggc gcgcagcgcg cgcatcgcgc tgccccgcga cctgtttctg
     9301 cccgagcgtg tgaattcgca gggattcgat ctgagcgacc gcctggcgct cggcgagctg
     9361 gcgagggcga tgtcggacct ggatggacgc cattgggacg ccgcgcccct cgtgccgggc
     9421 cgcgcaccgg gcgcgccgcc ggagcccgtt tactgccccg cgcggcgcag ccgcgtgatc
     9481 ggtgaagtgc gtccggcgac cgccggcgac gtggaagccg cgatgaagac ggcgatgggc
     9541 gctttctcat cctggacttc ccggcgcgtg gacgagcgcg cccggctgct cgagcaggcg
     9601 gccggcgcgc tcgagcgcgg gcgcgcggag ttcatccatc tgctcatcca cgaaggcggc
     9661 aagacgatac cggacgcgct cgccgaagtc cgcgaagccg cggattgctg ccgctattac
     9721 gcggcgcagg cgcgccgcga tttcggcgcg cccgtggcgc tgccgggccc gaccggcgag
     9781 cagaaccagc tctcgctgca cggccgcggc gtattcgcgt gcatcagccc ctggaatttc
     9841 ccgctcgcga tttttctcgg gcagatcacc gccgcgctgg ccgctggcaa taccgtcatc
     9901 gcgaagcccg ccgagcagac gccgctcatc gccgctgccg ccgtccgcct cctgcacgag
     9961 atcggaatac cggcagaggt cctgtgcctg ctgccgggcc cgggggcgga gatcggcgca
    10021 agattggtgg ccgaccagcg tctcgccggg gtcgcgttca cgggctccac ggacaccgcc
    10081 tggcgcatca acgcggcgct cgctgcgcgg cgggcgccga tcgcccccct gatcgcggag
    10141 accggcggcc agaacgcgat gatcgtggat tcttccgcgc tgctcgaaca ggcggccatg
    10201 gacatcgtga cctcggcctt cgacagcgct ggccagcgtt gctcggcgct gcgcgtgctg
    10261 ctggtacagg acgaggtggc gccgcggctg atcgagatgc tgcgcggcgc gatggcggag
    10321 cttgcggtcg gcgatcccgc gcggctggac gccgacgtcg gtccggtgat agacgaggag
    10381 gcgcttggcg cgctggagaa ccacgtggcg gcaatggcgc gcgccgggcg caatgtgttc
    10441 cggctgcccc tgccggcaga ttgtgccggc ggcacgttct gcgccccgac cctgatcgag
    10501 atcaccggga ccgccctgcc cgaccacgaa gtcttcggtc ccatcctcca cgtcgcccgg
    10561 taccagcgcg accgcctcga gcaggtgctg gaaaccgtcc gcgacactgg ttacggcctg
    10621 acgctgggca tccacacgcg tcttgccggg cgcgcagccg aaatcgcacg gtcgctaccc
    10681 gtgggcaacg tctacgttaa ccgcaacatg atcggcgcgg tcgtcggcgc gcaaccgttc
    10741 ggcggccagg gattgtcggg aaccggaccg aaagccggcg gtccccacta tctgcagcgg
    10801 ttcgcggtcg agaagacgac gaccatcaat acgatggcgg ccggcggcaa cgcggcactg
    10861 ctcgctggcg aggactgcta atcgtccgcg gctcgggagt tgcattggca tctagccgcg
    10921 gggaaaagac gggagatcgc cagatcggga ggtcgggaga tcgaaataac gggaagtcgt
    10981 gaaatcggga ggtcgggaga tcggaaaatc gtcagagagg gttttcccct tccgctctcc
    11041 cgctctctcg ccctcacgga actgccccct cccgcgctca cgctctcccg ccctcacgag
    11101 gggctatcgc ccttctgttg agtgatggca acgtcacgcg cacaatgcaa atttacaaaa
    11161 cgccttccaa gccggcattc atgaattcct tcagctgctc tttgattttc ctgttgatgc
    11221 tggttacaca cgccacatcc aacgccgagg aacgcacgac ccagagtcga acggacagcg
    11281 attcgaagct tcaaccgtat atccgctgtg atggattcgc cggaggagtg cgtgcagtta
    11341 ttctggaccg gcgcccgcag actgcggaac catggcgtgc ggcggatctc ggtggcagca
    11401 gccagcgagt ttcggtagta gatggctacc gcgtgattta ctcatatgtc aggacctatg
    11461 ccttcgcgaa cctgaaggca gaacggtccg acccttcaag atatcccgag gataagcgta
    11521 tcgtcatttc ggaactggtg gagatggcaa agacagatgg cgccgtggat ctggtcgatt
    11581 tttcccatgg aggtttttcg ggccagatgc tgacgaaaag agcgttgggc ggacgcgccc
    11641 tggcgaaatc ccagatattc tcggatgaag actccatcat cgtaagtatc tttttcttga
    11701 atcacgcgcc ggaaaatcgg aagttccggt cctacgatga attcatctct ctgcgggatg
    11761 ccttcattcg tggctatatc gattgcgtgg cgaagaagaa atccggcgcc atccgctccc
    11821 agtaatcgaa ccgccgagat agggagagaa acgaaaacaa ttttgaaccg ccaagacgcc
    11881 aagagaaaca ataaataagt gcaattcctc atcacccatc actcatcacc catcaccggg
    11941 tcttcttgag ggatgacaac ggcaagcgca gaatgccgcc ttataagacg gctttcggtc
    12001 gtttacttca acgtaagccc accaacaggg agcgcaagtg ccaactgcga ggccaagagg
    12061 tcgtccaccg aaggcaaaac acctcgatac acttctcgcc aaccttgaag aggttacccg
    12121 tctgatggag attcacacca aggttgcagg acgaaccgtg ggagctaaac acaaggtcga
    12181 agtcctaaac aagagtggaa ttgttctgct ggttgcctgc tgggaagcct tcgtcgagga
    12241 cttggctggc gtatcgtttc gactcctgct tcggcgggcc aagcgcccag atgtttttcc
    12301 atcgaaagtg cttgcactag cttccaagaa tttgcgggaa gatagggatg agagacgcgt
    12361 ctgggacctt gcaggagatg gctggaaaac tgtcctgcgc agccatcagg aaaacgtcgt
    12421 taagcggtac ataggcaacc tcaatacgcc caagccgaac cagatagacg agctgtttgc
    12481 ttctgtcatc gcattgggca aagtctcaaa aaagtggtct tggcacagat gtagccctgc
    12541 aaaggcacga gcccggctca ttaaactcgt aacgctgcgc ggggcgattg ctcaccgcgt
    12601 gtcgactcct cgctcggtgc tcaagcgtga cgtcgagcgt gctgcgaagt tcgtgggtag
    12661 attagcggta gcaacgcaca atgccgtcaa tacgagctta cgcgttaggc tagggcatag
    12721 tccgtggcaa gattacgtgt accgcgatgc aggataacca ctcgttctac gccgacgccc
    12781 aaaaaaatgg gatcggagtc atttttaatt agcgaaactc tcggattgtg aaaatcactc
    12841 cgaccccatt tctccgatga gtaaacctac tgccgggcat ttgctgtgga ccgctttgat
    12901 tctggcgttc ttcttcatta tcgcagcctt ggtggaatat aaatttgtgg ggtggcaagt
    12961 taggaacgcg gctctctctg atctgaggga tgaagcccag caacttaaca atgcgattgc
    13021 attcgataag ggcataaacc ttgagcagtt caatcgcgca ttcgttgacg ctggcgacta
    13081 ctttgtcgtc cttagcgacg gggcaatcat cgacattgca tcaggctcca agggaatgct
    13141 gcgcggggtt ctaccaaacg ttcagccagc atttagcgta gaggacgcgt tacgtgctcc
    13201 tctgacgatc gatcacagtg ttgggggaaa agcagaatcg tggacagttc tggccaagaa
    13261 gcttgagggc ggcgttgcga tactaggggt ctccgcgttc gacgcgatgc cggccgccgc
    13321 cgatattctg caaagaaaca tacaacgtat aggacgtaga ttatcggatg tggtgcacct
    13381 gaatcccggc gtcttggaca attcactgca ttgggcagtc attagcgatg ctggtgagct
    13441 tataactgcc tttgggcgat tgcctctcaa gaccgatcca atggcaatcg gtaaacaaag
    13501 cacgagcaac cccgaagtca tgattgaagg aaagccatac ctcgtgctat acgccccatt
    13561 gctagacaaa tctggacagc ccgcgggaac agtaattctt cctcagagct tggaaaggga
    13621 gaaggaggtt ctctggaaag agcttttatt cgatgcgtca gtttccgcga cctcgtttgc
    13681 cgtttttctg attctcgcgg cgtggtactt ctcaaagcat gaaagagaga aacgggctat
    13741 cagagaacaa tttcaaaagt tcctgccgcc gaagatccaa gatgaggtcc tgaacgaccc
    13801 tagtcaagcg gcccttggag gattgcggcg agagatcact attctgtttt ccgatattcg
    13861 atctttcacc acgctttgtg agcgtctgcc gcctcagcag ctaacgcgac tcctacagga
    13921 gtactttgag gcaatgacag aggaggtgat gaaggaagag ggatatgtgg ataaatacat
    13981 cggtgacggg atcatggcct tttggggcgc gccaatagac cagaaggacc aagctgaccg
    14041 ggccgtacgc actgctcaag cgatgattcg gcggttaaga gagctgcaag accaatggcg
    14101 cgcggcaggc tatccaattt ttgatattgg aattggaatc aatctcggtg tggcgaccgt
    14161 cggaaatatg ggatcgtcca aacatttcga ttacaccgcc gtcggcgata cggtaaatgc
    14221 ggcgtcaagg cttgaacaac tcacaaaaac atacgcgaat cacatcatca tttctgaatc
    14281 gacaaagaac cagttagaaa ttcgccctcc tctcaaagac ctcggcgaag tgaaggtcat
    14341 agggaaagag aaccccgtcc acatatacga agtactcccg gactagcggt aacatgcctc
    14401 acagtaatgg cgtctttggc gttatcgggt gcgttatggg gtcaggctta gctatttgca
    14461 ttaagctgaa agagatcaga gctgaaatat cttgctccgg cacttcgtca ccattatctc
    14521 atcaccttca cctcgtcacc cttatctcat cacctctttc tagacagtgc gcaccgcgat
    14581 aattatcctc ggtggcttcc tcctgctagg aatctgcgtc ctcgtcgggc gtttgataag
    14641 cggcgacaac acgaaggcga tggttgtcgc cgcccaggtt ttcatcccga tctggcttgc
    14701 tgccgccggg gtcaacatgt gggtgggcgt agccaaggcc ggttattcgg tcgccgagga
    14761 gctgccaatc ttcctgctga tcttcgccct gcccgccgcc gcggcggcgt tcgtctggtg
    14821 gaagttctcc tgaaaggagt cacaatgcct ccggaactgg taaaaggaat aagctactgt
    14881 ccggttcgtt gaagcttgtg catcgaggag cggcgagatg aagcgtagcg catggatcgc
    14941 cctgatcctg ctggccggaa tgcagcaagc cgtgtttgcg gccgaggctc gactcaatga
    15001 gcagcaaaaa ttgggccggc ggctgttcga gcaatcgtgc ggggtctgtc atacccggcc
    15061 aacgctggtc tccggcatgt atggtccgga actgtcccga gagagtgcag gcgggcggga
    15121 agaccttatg cgcgagatca tcagaagtgg gacggcgcgc atgccgggct tcaagtatac
    15181 ctacaacccg gcgcagatcg ccgccatcac cgcttacctc aagaccgtgc cgccaggcac
    15241 gcaggacctt ccgaccgtcc ggaaacattg aacgagcccc gggccgataa cgataccgtc
    15301 catgatccga tcttactttg agaggacgcg atgaaaagtc cacgtagctt taccggcgcc
    15361 agcctgatcg ccctcgccgc cgctctgggc tccgcgacac tcgttcccgg cgcggctcat
    15421 gccgacgcgc tgctgaccgg ggcggtcacc tcggccgcgg gcgagaagat gggcggcgtg
    15481 acggtgtcgg ccaaggcgga aggctcgccg atcaccacga gcgtcttcac ggacgagacc
    15541 ggcagcttct atttcccgcc gctgccggac ggcaaatacc gggtctgggc gcaggcgctg
    15601 acctaccgaa cggccaaagg cgccgtcgag ttgcgcaaga cgacgcgccg tgatttcgtc
    15661 ctgcggccga tgaagaaccg ggaggactgg atccggcaat tgcccggcga cgagttcctc
    15721 gccgcgctcc ccggcgacac gccggaagac taccggatga agacccaggt tcggaagaac
    15781 tgcacgggct gccacagcgc gagctatccg ctgcagcacc ggttcgacga agaaggctgg
    15841 cgcaaggtcc tcgacctgat gaagcacgtg aacgtgctcg gcgtctactt cggtccggac
    15901 cacaaggcga cgcccaacat cgaattccac cagaaggagc tgacggccta tctcgcccgc
    15961 gcgcgcggcc cgggcgcaag ctcgatgaaa ttcaagctgc gcccgcgccc ctccggcgag
    16021 gcggcgcgcg ccgtgatcag ggaatacgac ttcccgatgg aaggcgggca cgcgcgatcg
    16081 atggacgggt ccgactggtc gctcgggact ccgtccaaca tgaaccatgt cgccggcgtc
    16141 cacgacgcgc agatggactt cgacggaaac atctggatca cgttttccca taccagcacg
    16201 gaaaccacga tcgcccgtat cgacggcaag accggcgcgg tgaagcattt caggctcgac
    16261 gatcaaagag ggatcgcgat cggctcgcac ggcatcaccc gcgatgaaaa cggcatcctc
    16321 tggttcaaca cccggtcgca cgtggagcgc atccacggca gcctgggcag gatcgacccc
    16381 aggacggaga agatcaccgt ctattctccg cccaagccca tgtccggaac gtctggaacc
    16441 ctggatgccg acatgaacgg caacgtctgg gtcacgtccc ccgatggggc gctgcggttc
    16501 aatatcaagg aagagaaatt caccgagttc aaatcggtga cctacaagaa tcagcacggc
    16561 accgcgaccg tctacgg
//
LOCUS       DBNGWZ010000027         3300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2269310, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000027 DBNGWZ010000000
VERSION     DBNGWZ010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2269310"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /gene="glyA"
                     /locus_tag="AC16CX_00755"
     CDS             complement(<1..1226)
                     /gene="glyA"
                     /locus_tag="AC16CX_00755"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3011072.1"
                     /translation="MYSLNDTVEHFDPELWGAIEQERRRQEDHIELIASENYASPRVL
                     AAQGSVLTNKYAEGYPGKRYYGGCEHVDVAEQLAIDRAKKLFHAGYANVQPHSGAQAN
                     SAVYYAALHPGDTLLGMALDAGGHLTHGARVSLSGRYFDAVTYGLHDETEEIDYAEVE
                     ELAHVKKPKLIIAGASAYSLIIDWKRFRAIADKCGALLMADVAHYAGLIAAGLYPSPI
                     GIADFVTTTTHKTLRGPRGGLILANAEHEKVLNSAVFPGTQGGPLMHVIAAKAVALKE
                     AMSKEFKEYQQQVLENALVMAKTLHERGYRIVSGRTESHLFLVDLRGKKLTGRDAEFA
                     LGRANITVNKNAVPNDPQKPMVTSGIRIGSPAMTTRGFKEIEAELLANLIADVLDAHD
                     NDKAIRRVAGEVKKLC"
     gene            1353..1907
                     /locus_tag="AC16CX_00760"
     CDS             1353..1907
                     /locus_tag="AC16CX_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3011073.1"
                     /translation="MKHLLALSRLIDSLNERVGRFTTWLVLACVLISAANAVARYGFS
                     FSSNAFLEIQWYLYSIVYLCAAGYALKHNAHVRIDLVAGHLSRRAQLWIDVFGGLFML
                     LPVCTIVLWFGWNAFLESYRINEMSGDAGGLARWPIKFVIPLAFLLLTLQGVSETIKR
                     IAVLRGLIAEEPSGGEAGARLKGP"
     gene            1995..>3300
                     /locus_tag="AC16CX_00765"
     CDS             1995..>3300
                     /locus_tag="AC16CX_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3011074.1"
                     /translation="MAFELMAPLMFAGLAAFLLLGYPVAFALSANGLLFAFIGIELGM
                     LQPELLQALPQRLFGIMQNQLLLAIPFFTFMGLVLERSGMAEDLLDTIGQLFGPIRGG
                     IAYAVIFVGALLAATTGVIAASVIAMGLISLPVMLRYGYSRQLATGVICASGTLAQII
                     PPSIVLIVLADVLGASVGDIYLGAVLPGVMLVGLLMLYVMAASFAKPGVAPALPPEAR
                     TLRGAALYRHALFVMAPPLVLIFLVLGSIFLGIATPTEGGALGAVGSLALAWARGRLN
                     PKMLRGALDSTTRLASFVMFILIGSTVFALVFRAVDGDLWVERLFSLVPGGVWGFIIV
                     VNLLIFVLGFFIDFFEIAFILLPLIGPVAAKLGLDMVWFAVMIGMNMQTSFLTPPFGF
                     SLFYLRSVAPREVKTLDIYKGIVPFVCVQLVALAIVIAWPGLV"
BASE COUNT          452 a         1090 c         1062 g          696 t
ORIGIN      
        1 tcgcagagct ttttcacttc ccccgcgacg cgccggatcg ccttgtcgtt gtcatgggcg
       61 tcgagcacgt cggcgatgag gttcgcgagc agctcggcct cgatttcctt gaagccgcgc
      121 gtggtcatgg ccggggaccc gatgcgtatg ccgctggtga ccatcggctt ctgcggatcg
      181 ttgggcacgg cattcttgtt gaccgtgatg ttggctcttc cgagcgcgaa ctcggcatcg
      241 cgtccggtga gcttcttgcc gcgcaggtcc accaggaaca ggtgggactc ggtgcggccc
      301 gacacgatgc ggtagccgcg ttcgtgcagc gtcttggcca tgacgagcgc gttttcgagc
      361 acctgctgct ggtactcctt gaactccttc gacatcgcct ccttgagcgc gaccgccttg
      421 gcggcgatca catgcatcag cggacccccc tgtgtgccgg ggaaaaccgc cgaattcagc
      481 actttctcgt gctcggcgtt cgccaggatc aatccgccgc gtggcccccg cagcgtcttg
      541 tgcgtggtgg tggtcacgaa atcggcgatg ccgatcgggc tggggtacag ccccgccgcg
      601 atcaaacccg catagtgcgc gacgtccgcc atcagcagcg cgccgcactt gtccgcgatc
      661 gcgcggaaac gcttccagtc gatgatcagc gaataggccg aagcgccggc gatgatgagc
      721 ttcggctttt tgacgtgcgc gagctcctcg acctcggcgt agtcgatctc ctcggtctcg
      781 tcatgaagcc cgtacgtcac cgcatcgaag tagcgtccgg agaggctcac gcgcgcgccg
      841 tgcgtgagat ggccgccggc gtcgagcgcc atgccgagga gggtgtcgcc ggggtgcagc
      901 gccgcgtaat acaccgccga gttcgcctgc gcgcccgaat gcggctgcac gttggcgtag
      961 ccggcgtgga acagcttctt cgcgcggtct atcgcgagtt gctccgcgac gtccacgtgc
     1021 tcgcagccgc cgtagtagcg cttgccgggg tagccttcgg cgtacttgtt ggtgagcacc
     1081 gagccctggg ccgcgagcac gcgcgggctg gcgtagttct ccgaggcgat caactcgatg
     1141 tggtcttcct ggcgccggcg ttcctgctcg atcgcgcccc acagctcggg atcgaagtgt
     1201 tcgacggtgt cattgagcga gtacatggga aaaggcgttt cgtcttgtaa actgttgatt
     1261 ttagcatcga agcgtcggcg acgcgcgatc acggttcggc ttggcccgcc cgtcccgttg
     1321 cggcacaatc cggtgcgcct ccgatagaga ccatgaagca cctgctcgcg ctttcgcggc
     1381 tgatcgattc cctcaacgag cgcgtcggcc gcttcaccac ctggctcgtg ctcgcctgcg
     1441 tgctgatcag cgccgccaac gccgtcgcgc gctacggatt cagtttcagt tccaacgcat
     1501 ttctcgagat ccagtggtac ctctattcga tcgtctacct gtgcgcggcc ggctacgcgc
     1561 tgaagcataa cgcccacgtg cggatcgacc tcgtcgccgg ccatctctcg cgccgcgccc
     1621 agttatggat cgacgtcttc ggcgggttgt tcatgctgct gccggtgtgc acgatcgttc
     1681 tgtggttcgg ctggaacgcc ttcctcgagt cctaccgcat caatgagatg tcgggcgacg
     1741 cgggcgggct cgcgcgctgg ccgatcaagt tcgtcatccc gctcgccttc ctgctgctca
     1801 ccctgcaagg cgtctcggaa acgatcaaac gcatcgccgt cctgcgcggc ctcatcgccg
     1861 aggagccgtc tggcggcgag gcgggggcgc ggctcaaagg gccgtgagcg gtcagctgcg
     1921 agcggtgagc ggcagacgac gacttaaccg cttactgctc accgcttacc gcatacgagt
     1981 gaaagcgtag cgcaatggca ttcgagctca tggccccgct catgttcgcg gggctcgccg
     2041 cgtttctgct gctcggctac cccgtggcat ttgcgctgtc ggcaaacggg ctgctgttcg
     2101 cgttcatcgg catcgagctg gggatgctcc agcccgagct gctgcaggcg ctgccgcagc
     2161 ggttgttcgg catcatgcag aaccagctgc tgctcgccat cccgttcttc accttcatgg
     2221 ggctggtgct cgagcgcagc ggcatggccg aggacctgct cgacaccatc ggccagctgt
     2281 tcggcccgat ccgcggcggt atcgcctacg cggtgatctt cgtgggcgcg ctgctcgccg
     2341 ccaccaccgg cgtgatcgcc gcctcggtga tcgcgatggg cctgatctcg ctgccggtga
     2401 tgctgcgcta cggatactcg cgccagctcg ccaccggcgt catctgcgct tcgggcaccc
     2461 ttgcccagat cattccgcct tctatcgtgc tgatcgtgct cgccgacgtg ctgggggcat
     2521 ccgtgggcga tatctacctg ggcgccgtgc ttcccggcgt catgctggtc ggattgttga
     2581 tgctgtacgt gatggccgct tccttcgcga agcccggcgt cgcgccggcg ctgccgccgg
     2641 aagcgcgcac gctgcgcggg gcggcgctct accggcacgc cctgttcgtg atggccccgc
     2701 cgctcgtgct gatcttcctc gtgctgggct cgatcttcct gggcatcgcc actccaaccg
     2761 agggcggagc gctcggagcg gtgggatcgc tcgcgctggc gtgggccagg ggccggctca
     2821 atccgaagat gctgcgcgga gcgctcgatt ccaccacccg gctcgcaagc ttcgtaatgt
     2881 tcatcttgat cggctcgacc gtcttcgcgc tggtgttccg ggcggtggac ggcgacctgt
     2941 gggtcgagcg gctgttctcg ttggtgccgg gcggcgtgtg gggtttcatc atcgtcgtca
     3001 acctgctcat cttcgtgctc ggctttttca tcgatttctt cgagatcgcc ttcatcctgc
     3061 tgccgctgat cggaccggtc gccgccaagc tcgggctgga catggtgtgg ttcgcggtga
     3121 tgatcggcat gaacatgcag acctccttcc tcacgccgcc cttcggcttc tcgctgttct
     3181 acctgcgcag cgtggcgccg agggaagtaa aaaccctcga catctacaaa ggcatcgtgc
     3241 ccttcgtctg cgtgcagctc gtcgcgctgg caatcgtcat cgcctggccg gggctggtga
//
LOCUS       DBNGWZ010000028         3499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2143524, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000028 DBNGWZ010000000
VERSION     DBNGWZ010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2143524"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="AC16CX_00770"
     CDS             complement(<1..683)
                     /locus_tag="AC16CX_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3011075.1"
                     /translation="MRLILMVLMTFSFVAAAPALAQTYPARTVRLIAPFAPGGATDVL
                     ARLTAQKLGERWGQSVIVDNRVGAGGHIGAELASRAAPDGYTLLVAGTPHAIGMSLYK
                     KLAYDLGKDLIGANRIATYPSAIAVHPSLPVKNVKELIALAKARPGQLNFGSAGIGSP
                     NGLALELFKTMAKVSMVHIPYKGGSGQMVTELLSGQVQLASIGLPPAMPYVKSGRLRV
                     IAVTGMTRSA"
     gene            827..2155
                     /locus_tag="AC16CX_00775"
     CDS             827..2155
                     /locus_tag="AC16CX_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3011076.1"
                     /translation="MNAPQQVPSLMKNVSRISHLDLPGAGQVYVTGKYAYVGHITNKE
                     GLGTSILDVSDPKKPRLLSQLPVGDPNSHSHKARVIGDILIVNVEQNMTAIGRKADEL
                     PKLRTQLRAALGRDPTHAELAEKLGVKASDIPAVEAMEKAPYDQGGFKIYDVSDRTKP
                     RLITHRRTHGRGVHRFDIDANYAYISTEMPGYIGNILVIYDIRNPARPEEVSRWWLPG
                     QHNAGGEKPTWPGRQHRLHHALRIGDRLYAGCWHGGVRVIDASDIRSPKTVGAYNYHP
                     PFPEPSHTFMGLPKPLDGRQIAIAIDEEDHAQSAEEMAKRRGRPHGCLWVFDITDVKN
                     IKPLSIYEVSELDSPWSRAAPGRFGAHQFQEHMKGGDTLVYCAWFAGGLRIVDIADPL
                     APQEAGYYVPEPAPGKVAPQSNDVDVDERGLVYLVDRYNGFDILEFKKNA"
     gene            complement(2247..2942)
                     /locus_tag="AC16CX_00780"
     CDS             complement(2247..2942)
                     /locus_tag="AC16CX_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3011077.1"
                     /translation="MTTLKVLSAGAVKGGVAKIVAEFERVTSTRVTVDFAPAPDLRDR
                     FLAGEEADVIILAQSMMDQVAKRGKILPDSRALLGSSRMGVAVHAKAPSPDLSDTAAF
                     KKLIQGATQVIYNKASSGLYAAKLLEKLGLKKGLGSKIVVVDSGAAIMETVAAQPPGA
                     VGLAQMSEIMVLVEKGCAVKLAAPLPDEIQNVTSYDAAATVASKAPDVARKLAASLTS
                     EAAKKVFAATGIS"
     gene            complement(3023..>3499)
                     /locus_tag="AC16CX_00785"
     CDS             complement(3023..>3499)
                     /locus_tag="AC16CX_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3011078.1"
                     /translation="VEEGGVNVGRVGLGVVIRPGAPVPVISSAEAIRRTVLEAESVVF
                     NRASSGLYFESLLKKMGIYDQIEPKAARYATGAEVMEHVLKGKGKEVGFGPITEILQY
                     KKQGLRLVGPLPAEIQNYTAYVAVPMTASANKDVAAAFVRFLGGAGKPLFVAAGID"
BASE COUNT          644 a         1199 c         1051 g          605 t
ORIGIN      
        1 gctgaacgcg tcatgccggt cacggcaatg acgcggagcc tcccggactt gacgtacggc
       61 atcgccggcg gcagcccgat cgaggcgagc tgcacctgtc cggacagcag ctcggtgacc
      121 atctgcccgg agccgccctt gtagggaatg tgcaccatgc tgaccttcgc catggtcttg
      181 aacagttcca gcgcgagccc gttcggcgag ccgattcccg ccgacccgaa gttgagctgc
      241 ccggggcgcg ctttcgccag cgcgatcagc tccttcacgt ttttcacggg cagcgacgga
      301 tgcaccgcga tcgcgctggg ataggtggcg atgcggttcg ccccgatcag atccttaccc
      361 aggtcgtatg cgagcttttt gtacaggctc atgccgatcg cgtgcggcgt gcccgccacc
      421 agcaacgtgt agccgtccgg tgcggcgcgg ctcgccagtt ccgctccgat atgtcccccg
      481 gcgccgacgc ggttgtccac gatgaccgac tggccccagc gttcgccgag cttctgtgcc
      541 gtgagccgcg cgagcacgtc ggtcgcgccg ccgggtgcga acggggcgat cagccggacg
      601 gtcctggcgg gatacgtctg cgctaaggcg ggcgctgccg ccacgaatga gaacgtcatc
      661 agcaccatca aaattaaacg catggcattc acctctcttt cggcccgcgc ccgcctgaat
      721 tgaacaataa tgagtatcca catcatgaat cgcgcgggcc gctgggaccg cgcgattcat
      781 gattcattgt agaatcgtga cccgcaccgc acaacaggaa tccgtcatga acgcacctca
      841 gcaggtcccc tcgctcatga agaacgtcag ccgcatctcg cacctcgacc tgcccggcgc
      901 cggccaggtc tacgtgacgg ggaaatacgc ctatgtcggc cacatcacga acaaggaggg
      961 cctcggcacc agcatcctcg acgtgtccga cccgaagaaa ccacgcttgc tctcgcagct
     1021 gcccgtcggc gacccgaact cgcacagcca caaggcgcgc gtcatcggcg acatcttgat
     1081 cgtcaacgtc gagcagaaca tgaccgcgat cggccgcaag gccgacgagc tgcccaagct
     1141 gcgcacgcaa ttgcgcgccg cgctcgggcg cgatcccacc catgcggagc tcgcggagaa
     1201 actgggcgtg aaggcatcag acatcccggc ggtcgaggcg atggaaaagg cgccgtacga
     1261 tcagggcggg ttcaagatct acgatgtctc ggaccgcacc aagccccggc tgatcacgca
     1321 ccgcaggacc cacggccgcg gcgtgcaccg cttcgacata gatgcaaact acgcctacat
     1381 ttcgaccgag atgccgggtt acatcggcaa catcctcgtg atctacgaca tccgcaaccc
     1441 ggcgaggccc gaggaagtct cgcgctggtg gcttccgggg cagcacaacg cgggcggcga
     1501 gaagccgacc tggcccggcc ggcagcaccg cctgcaccat gcgctgcgga tcggcgaccg
     1561 gctgtacgcg ggctgctggc acggcggcgt gcgcgtcatc gacgcgagcg acatccgcag
     1621 tccgaagacg gtcggcgcat acaactatca cccgccgttc ccggagccct ctcatacttt
     1681 catgggcctg cccaagcccc tggacggcag gcagatcgcg attgcgatcg acgaggagga
     1741 ccacgcccaa agcgcggagg agatggcgaa gcgccgcggc cgcccccacg gctgcttgtg
     1801 ggtgttcgac atcaccgacg tcaagaacat caagccgctg tcgatctacg aagtaagcga
     1861 gctcgactcg ccgtggagcc gcgccgcgcc ggggcgcttc ggcgcgcacc agttccagga
     1921 acacatgaag ggcggcgaca cgctggtcta ttgcgcgtgg ttcgccggag ggctgcgcat
     1981 cgtggacatc gccgatccgc ttgccccgca ggaagccggc tactacgtcc ccgagcccgc
     2041 gccgggcaag gtcgcgccgc agtcgaacga cgtggacgtg gacgagcgcg gcctcgtgta
     2101 tctcgtcgac cgctacaacg ggttcgacat cttggaattc aagaaaaatg cgtgataagt
     2161 gacgggtgat aagtgataag tctcccgcgt agcgggccaa gcccctatca cctatcactt
     2221 atcacctatc acgctccctc cttcagctag ctgattcccg tcgcagcgaa gaccttcttc
     2281 gccgcctccg aagtgaggct cgccgccagc ttgcgcgcca catccggcgc cttgctcgcc
     2341 accgtggcgg ccgcgtcgta gctcgtcacg ttctggatct cgtcgggcag cggcgccgcg
     2401 agcttgactg cgcagccctt ctcgaccagc accatgatct ccgacatctg tgccagcccg
     2461 acagccccgg gaggctgtgc cgccaccgtt tccatgatgg cggcgccact gtcgacgacg
     2521 acgattttcg atcccaggcc ttttttcagc ccgagctttt ccaggagttt tgcggcatag
     2581 agcccgctgg acgccttgtt gtagatcacc tgcgtggcgc cctgaatcag cttcttgaaa
     2641 gccgccgtat ccgaaagatc gggggatggg gccttggcat gcactgcgac tcccatgcgg
     2701 gaactgccca gcagcgcgcg gctgtccgga aggatttttc cccgcttagc aacctggtcc
     2761 atcatcgact gcgccaggat gatgacgtcc gcctcctcac ccgcgaggaa gcggtcgcgc
     2821 aaatcgggtg ccggcgcgaa atcaaccgtg acgcgcgtgc tggtcacgcg ctcgaactcg
     2881 gccacgatct tcgctacgcc acccttgacc gcccccgcgg aaagcacctt cagtgtcgtc
     2941 attgtgactc ctgctatcag ccgttagcta tcagcaaaaa cgtcgggcta ttgctgaacg
     3001 ctgaaagctg actgctgaac gcttaatcaa ttcccgctgc gacgaataac ggcttgccgg
     3061 cgccgccgag gaagcgaacg aacgccgcag caacatcctt gttggcgctc gcggtcattg
     3121 gcaccgcgac gtaggccgtg tagttctgga tctcggccgg cagcggcccg accaggcgca
     3181 ggccttgttt cttgtactgc aggatttcgg tgatcgggcc gaagccgacc tctttccctt
     3241 tgcccttcag cacgtgctcc atgacctcgg cgccggtggc gtaacgagcg gccttgggct
     3301 cgatctggtc atagattccc atcttcttca gcagactctc gaaataaagg ccgctggagg
     3361 cgcggttgaa gacgacggat tccgcttcca gcacggtgcg cctgatcgct tccgcgctcg
     3421 agataaccgg caccggcgcg cccggacgga tcaccacgcc caaccccacg cgcccgacgt
     3481 tgacgccgcc ctcctcgac
//
LOCUS       DBNGWZ010000029         5650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_632441, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000029 DBNGWZ010000000
VERSION     DBNGWZ010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_632441"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /gene="pyrC"
                     /locus_tag="AC16CX_00790"
     CDS             complement(<1..971)
                     /gene="pyrC"
                     /locus_tag="AC16CX_00790"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3011079.1"
                     /translation="MAEGTSASRITIRKPDDWHLHVRDGSMLKAVLPFTARHFGRAII
                     MPNLLPPVCTVKEAEAYRARILQALPAGAKFEPLMTCYMTHDTNPDEVEAGFKAGVFT
                     GVKIYPANVTTHSAAGVSDYRKIRRVIERMEKIGMRLLIHGEVIDPEVDVFDREAVFI
                     ERELEPLTRDFPGLKVILEHVSSKVGVEFVRSKTGQVGGTITPYHLMLTRTDWLGWGN
                     RPYLYCAPVIKTAKDRAALRAAATSAGSNFFLGTDSAPHPVARKLDVVGAAGLFNSPV
                     AIESYAQVFAEENALDKLEAFASLNGPRHYGLPPNREAITLERAPWTA"
     gene            complement(1013..1960)
                     /locus_tag="AC16CX_00795"
     CDS             complement(1013..1960)
                     /locus_tag="AC16CX_00795"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR3011080.1"
                     /translation="MVDLSVKIGSVTLPNPILPASGTFSAEFSRVIDLNRLGALVTKT
                     VSRNYRPGNPPPRVSEVEAGMINSIGLPTKGLQYFLDVQLPEYKRFRPPLVASITSTD
                     IEDFAAMAREVSVPGVDAIELNISCPTRKPGGGNFALNEEHTYKVVRRCREATGKPLW
                     AKLSPNAGEISAVAEAAEKAGANAITVSNTILGLKINLETFRSCIGQGYGGISGPGVK
                     PIILRMVHQCSKCVKIPIIGCGGITKPEDVVEYLLAGATAVEVGYLNFRNPTGMIALI
                     DGLQKWCDGRGIKRVADLTGAMLDHPPRDTYEAGMMGIS"
     gene            complement(1954..2820)
                     /locus_tag="AC16CX_00800"
     CDS             complement(1954..2820)
                     /locus_tag="AC16CX_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR3011081.1"
                     /translation="MPEHVYATAAVATGKPGNGHSPANCPVEETLCPVVAHEWVNDEY
                     KHLVVKATPKALAAQPGQFFHLLCPSPDEGEVWFRRPQSVYRVARSSGRIEFLYKVVG
                     RGTRGLAMLEPGDTLNMLGPLGVGFRLEPAWKNVVVLGRGVGLATMAPISQLAGERGV
                     RVTAILSARREDLAMAADLFESVGTVIKVLDTDGTSAVENVEAILRRLIAEKKADAFF
                     TCGSNRLMQLMKRLGKEHGIPGQMAMEQIMACGLGPCYICVRTFEVNGKLEMRRVCIE
                     GPVFDLQECLGW"
     gene            complement(2918..3070)
                     /locus_tag="AC16CX_00805"
     CDS             complement(2918..3070)
                     /locus_tag="AC16CX_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011082.1"
                     /translation="MTGIIKRKLIFARMFSSAREFVEQRPASLSAACRSGKSGRLLIP
                     LHRNSV"
     gene            3069..4028
                     /locus_tag="AC16CX_00810"
     CDS             3069..4028
                     /locus_tag="AC16CX_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011083.1"
                     /translation="MLAPLFVIAASLSAAASAAAADYPSRPLRLLVPFPPGGGVDLLA
                     RMLGTRLSEALGQQVVVDNRAGGGGVIATDLAAKAPADGYTMLLGFIGPLAISPTISK
                     LPYDPVRDFAGLDLLASSYHIVVINPSLPVRSVKEFIALAKSQPGKLNYASSGSGTNL
                     HLAAELFKMVVGINLVHVPYKGAGPAASAVLSGESHLLFGSITSSMPFVRANRLVALA
                     VTSPQRSPLAPEIPTLIESGVSGVNVPSWYTLVVPARTPREVAGKLRLELKRIAANAE
                     FREQIARQAIEARILAPGEFPGFLKAELEKWGKVARAVGIKPE"
     gene            complement(4183..>5650)
                     /locus_tag="AC16CX_00815"
     CDS             complement(4183..>5650)
                     /locus_tag="AC16CX_00815"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3011084.1"
                     /translation="GDSGAPVSKTVGNYGDLRGWIEQVDKMGELLKVSGAHWDAEMGS
                     ITQMLTEKSRGDAPAILFDEIPGYPKGYRTLYGHFSSIRRVALTLGLPLEYERKVDIV
                     KQYHERLQSMKPLPPRWVKDGPMLENVIEGGKVDVLKFPVPRHHEQDKARYIGTANCV
                     ITQDPDAGWFNLGCYRNQVYDGKTIGCQITEGKHGRIHRDKYFERGQSMKVVVLVGQD
                     PLLFMLAASPLPEGISELDFAGGLRGEPVAVVRGPYTGFPIPAHAEIAIEAEAVPGRV
                     KEEGPFGEWMGYYSDDTVPRPYLDVKTIMHRNNPILTCAPQHKPVDETGLLKGIAGSA
                     QIWRALEASGLPDILGVWNHEGGPATRFTVIQIKQRYPGHARNVLHVASNCQGGAYAG
                     KWTVVVDEDVDAGDLDQVLWAMCTRFDPVTDIDLIQKAWASKRDPLYLPGNFNNRILI
                     DACIPYDMKLKGKFPPVVDVSPELRAKIRAKFSQIRFP"
BASE COUNT          911 a         1844 c         1797 g         1098 t
ORIGIN      
        1 gcggtccacg gcgcgcgctc gagcgtgatc gcctcccggt ttggcggcaa tccatagtgc
       61 cgcggtccat tgagcgaggc aaacgcttcg agcttgtcga gcgcgttctc ctccgcgaac
      121 acctgagcgt aggattcgat cgccaccggc gagttgaaca ggcccgcggc gccgaccacg
      181 tcgagctttc tcgccaccgg gtgcggcgcg gagtccgttc cgaggaagaa gttgctccca
      241 gcggaggtcg cggctgcgcg taacgccgct cggtccttcg cggtcttgat gaccggcgcg
      301 caatacaggt acgggcggtt gccccagccc agccagtcgg tgcgggtgag catgaggtgg
      361 tagggcgtga tggtaccgcc cacctgcccg gtttttgagc gcacgaattc gaccccgacc
      421 ttcgacgaaa cgtgctcgag tatcaccttg agccccggga aatcgcgcgt gagcggctcg
      481 agctcgcgct cgatgaacac cgcttcgcgg tcgaaaacgt cgacttccgg gtcgatcacc
      541 tcgccgtgaa tcagcaggcg catgccgatt ttctccatgc gctcgatcac ccggcggatc
      601 ttcctgtagt ccgagacgcc ggccgcggag tgggtcgtca cgttcgccgg gtaaatcttg
      661 acgccggtga aaacacccgc cttgaacccg gcttccactt cgtcgggatt cgtgtcgtgc
      721 gtcatgtagc aggtcatcag cggctcgaac ttcgcgcccg cgggaagcgc ttgcaggata
      781 cgagcgcgat acgcctccgc ctccttcacc gtgcacaccg gcggcagcag gttgggcatg
      841 atgattgccc gcccgaagtg ccgcgcggtg aacggcagca ccgccttcag catggagccg
      901 tcgcgcacgt gcaggtgcca gtcgtccggc ttgcggatcg tgatacgtga agccgaagtc
      961 ccttccgcca ttcgccttcc gcctttcgac tttcgacttt cgactttcga cttcagctta
     1021 ttcccatcat gccggcttcg taggtgtcgc gcggcgggtg gtcgagcatc gcgccggtga
     1081 ggtcggcgac gcgcttgatg ccgcggccat cgcaccattt ctgcagcccg tcgatcagcg
     1141 cgatcatgcc ggtgggattg cggaagttga ggtagccgac ctcgaccgcg gtcgcgccgg
     1201 cgaggagata ctcgaccacg tcctccggct ttgtgatgcc gccgcagccg atgatcggga
     1261 tcttcacgca cttcgagcac tggtggacca tgcgcaggat gatgggcttc acgcccgggc
     1321 cggaaatgcc cccgtaaccc tggccgatgc aggaacggaa tgtctcgagg ttgatcttga
     1381 gccccaggat cgtgtttgac acggtgatcg cgttcgcccc ggccttctcg gcggcttccg
     1441 cgaccgcgct gatctcgccg gcgttgggcg agagcttggc ccacagcggc ttcccggtcg
     1501 cttcccggca gcgccttacc accttgtagg tgtgctcctc gttgagcgcg aaattgcctc
     1561 cgcccggctt gcgcgtcggg cacgagatgt tcagctcgat cgcatccact ccgggaacac
     1621 tcacctcgcg cgccatggcg gcgaaatcct cgatgtcggt ggaggtgatg ctggcgacga
     1681 gcggcggccg gaagcgcttg tattccggga gctgtacgtc gagaaagtac tgcagcccct
     1741 tggtcggcag cccgatcgag ttgatcatcc ccgcctcgac ctccgagacg cgcggcggcg
     1801 ggttgcctgg gcggtaattg cgcgacacgg tcttagtgac gagcgcgccg aggcggttca
     1861 agtcgatgac ccgggagaac tccgccgaga acgtgccgga ggccggcagg atcgggttcg
     1921 ggagcgtcac gctcccgatc ttgactgaca ggtctaccat ccgaggcact cctgaaggtc
     1981 gaacacgggg ccttcgatgc acacgcggcg catttccagc ttgccgttga cttcgaacgt
     2041 gcgcacgcag atgtagcagg gccccagccc gcacgccatg atctgttcca tcgccatctg
     2101 gccggggata ccgtgctctt ttccgagccg cttcatgagc tgcatgaggc ggttcgagcc
     2161 gcaagtgaag aacgcgtccg ccttcttctc cgcgatcagc cgccgcagga tggcctcgac
     2221 gttctcgacc gcgctcgtac cgtcggtatc gagcaccttg atgacggtgc cgacgctctc
     2281 gaacagatcg gcggccatcg ccagatcctc gcggcgcgcg ctgaggatcg ccgtcacccg
     2341 cacaccgcgc tcgccggcga gctgggaaat cggcgccatc gtggcgagcc ccacgccgcg
     2401 cccgagcacc accacgttct tccacgccgg ctccagtctg aagccgacgc ccagcggccc
     2461 gagcatgttg agcgtgtcgc cgggctcgag cattgccagg cccctcgtac cgcggccgac
     2521 caccttgtac aggaactcga tgcgcccgct cgagcgcgca acgcggtaga cgctctgcgg
     2581 acggcggaac cagacctcgc cttcgtcggg cgacggacac agcaggtgaa aaaactggcc
     2641 gggttgcgcg gcgagcgcct tgggcgtcgc cttgacgacg aggtgcttgt actcgtcgtt
     2701 gacccactcg tgggcgacga ccgggcacaa ggtttcttcg accgggcagt tggcgggaga
     2761 atgcccgttg ccgggcttcc cggtcgcaac cgcggccgtg gcgtaaacat gctctggcat
     2821 ggtttcggtt attccagatg gattgggtga tccggctggg tgaataagcg caatccagcc
     2881 gtccactata agaaatcggg gtcttcttct tgatatttta gacagagttg cgatgtaatg
     2941 gaatcagcag cctgccggac ttaccgcttc ggcaggctgc tgataagctg gctggccgct
     3001 gttcgacaaa ctcccgggcg gaggaaaaca tgcgagcaaa aatcagtttg cgcttgatga
     3061 tgcccgtcat gctagcgccg ctctttgtga tcgcggcttc tttatcagcc gccgcgtctg
     3121 ccgcggcggc tgattatcca tcgcgaccgt tgcggctgct cgtgccgttc ccgcccgggg
     3181 gtggcgtgga tctcctcgcg cgcatgctcg ggacacggct ctcggaggcg ctgggccagc
     3241 aggtggtcgt ggataatcgc gcaggcggcg ggggcgtcat cgcgaccgac ctcgccgcca
     3301 aggcgccggc cgatggatac accatgttgc tcggcttcat cgggccgctc gccatctcgc
     3361 ccacgatctc gaagctgccg tacgacccgg tcagggattt cgctggcctc gatctcctcg
     3421 cctcctccta ccacatcgtg gtcatcaatc cttccctgcc ggtacgttcg gtaaaggaat
     3481 tcatcgcact tgcaaaaagc cagcccggca agctcaacta cgcgtcgagc ggcagcggca
     3541 ccaacctgca tctcgcggcg gaactgttca agatggtggt cggcatcaat ctcgtccacg
     3601 tgccgtacaa gggcgccggg cccgcggcga gcgcggtcct ctcgggtgaa agccacctgc
     3661 tattcggctc gatcacctcg agcatgccgt tcgtgcgcgc caaccgcctg gttgcgctgg
     3721 cggtcacaag cccgcagcgc tcgccgcttg ccccggagat cccgacgctg atcgagagcg
     3781 gcgtgagcgg cgtcaatgtc ccgtcctggt acacgctggt cgtgccggcc cgcaccccgc
     3841 gcgaagtggc gggcaagctg cgcctggaac tgaagcggat tgcggcgaac gcggaattcc
     3901 gcgagcagat cgcccgtcag gctatcgagg cgaggattct tgctccgggt gagtttcccg
     3961 gctttctcaa ggcggaactc gagaagtggg gcaaggtggc gcgggcggtg ggcatcaagc
     4021 ccgaataaat tctcgcgccc ctggtgcgcg agaatttatt aacgaaatac tcaatgtgcg
     4081 cttagacgcg ccatgagcgc atttttcagg tagccgggga atgaattatt ttattgtgga
     4141 gttttcgata aggaatttgt cgcactgctg tgcgacaaat tcctatggga agcggatctg
     4201 gctgaatttc gcccggatct tcgcccgcag ctcggggctc acgtccacta ccggcgggaa
     4261 tttgcccttc agcttcatgt cgtaggggat gcaggcgtcg atcaggatac ggttgttgaa
     4321 gttgcccggc aggtagagcg gatcgcgctt cgaggcccat gccttctgga tgaggtcgat
     4381 gtcggtcacc gggtcgaagc gcgtgcacat cgcccacagc acctggtcga gatcgccggc
     4441 atcgacgtcc tcgtccacca ccaccgtcca tttgccggcg taggcgccgc cctggcagtt
     4501 cgacgcgacg tgcagtacgt tgcgcgcgtg ccccggatag cgctgcttga tctggatcac
     4561 ggtgaagcgc gttgccggcc caccctcgtg gttccacacg cccaggatgt ccggcaaccc
     4621 cgaggcttcg agcgcgcgcc agatctgcgc ggagcccgcg atccctttca ggagtcccgt
     4681 ctcgtccacc ggcttgtgct gcggcgcgca cgtgaggatc ggattgttcc ggtgcatgat
     4741 cgtcttcacg tcgagatacg gccgcggcac cgtgtcgtcc gagtagtagc ccatccattc
     4801 gccgaacggt ccttcctcct tgacccgccc gggaaccgct tcggcctcga tcgcgatctc
     4861 ggcgtgcgcg ggaatcggga agcccgtata gggtccgcgc acgacagcaa ccggttcgcc
     4921 gcgcagcccg ccggcgaaat ccagctcgct gatgccttcc ggcagcgggc tcgccgccag
     4981 catgaacagc agcgggtcct ggcccaccag caccaccacc ttcatggatt gcccgcgctc
     5041 gaagtacttg tcacggtgga tgcggccgtg cttgccctcg gtgatctggc agccgatggt
     5101 cttgccgtcg tagacctggt tgcggtagca gccgaggttg aaccagccgg cgtccgggtc
     5161 ctgcgtgatc acgcagttcg ccgtgccgat gtagcgcgcc ttatcctgct cgtggtgtcg
     5221 cggcaccgga aacttgagca cgtccacctt gccgccctcg atcacgttct ccagcatcgg
     5281 gccgtccttc acccagcgtg gcggcagcgg cttcatgctc tgcaggcgtt cgtgatactg
     5341 ctttacgatg tccaccttgc gctcgtattc gagcggcaag ccgagtgtca gcgcgacgcg
     5401 gcggatcgag gagaaatgcc cgtagagcgt gcggtagcct ttcgggtagc cggggatttc
     5461 gtcgaacagg atggcgggcg cgtcgccgcg gcttttctcg gtgagcatct gggtgatgga
     5521 ccccatctcc gcgtcccaat gggcgccgct caccttgagc agctcgccca tcttgtccac
     5581 ctgctcgatc catccccgca ggtcgccgta gttgcccacc gttttggaga cgggcgctcc
     5641 gctgtcgccg
//
LOCUS       DBNGWZ010000030         4777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2017762, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000030 DBNGWZ010000000
VERSION     DBNGWZ010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2017762"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..369)
                     /locus_tag="AC16CX_00820"
     CDS             complement(97..369)
                     /locus_tag="AC16CX_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HIR3011085.1"
                     /translation="MQFEWDPAKAAENLAKHGVSFEEAATVFRDALSATGPDPDHSVG
                     EERFITFGFSTSGRLLVVAHTEHGDIIRIISARLATAGERTIYEEG"
     gene            complement(409..630)
                     /locus_tag="AC16CX_00825"
     CDS             complement(409..630)
                     /locus_tag="AC16CX_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011086.1"
                     /translation="MKIATGRVIGGKVVLEGEPLAEGSVVTVVAREEDETFEVSPEEE
                     CALLEAIAQADRGQVVSWHELREQLRRYG"
     gene            921..2096
                     /locus_tag="AC16CX_00830"
     CDS             921..2096
                     /locus_tag="AC16CX_00830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011087.1"
                     /translation="MFVLNADQKAILDTVRRYVDQEVRPRSAMLDARQNPEDCFSWEL
                     VKKAHELGIRTMTLAEKWGGLGLDSLTTAMVVEELAVGDMGVAVVMAQTFKIAQLMQQ
                     TLNEEQQKRVLPAFARDPRGVLAIGMSEPGNGSDKHTNQPVPFQTGAERTRGGWIVNG
                     MKHFISNGNRASHYLVMVQTDRQKTMANGSTCFLLERGHPGFMIGRIHDKMGERLANN
                     AELIFQDCTIPDGNVVGEVNKGSEIFARFSPQSNAYAGATVLGVAVALHRKAIEWAKL
                     RVQGGKRLVEHDSIRAQLAEMRMLIDASRSYIHRACWFADRPQHGWDKTLGALPKAMA
                     AQAAWKIATWTMEIHGGHGYMKEAGVEKLVRDAAAFLHSDGVNRTLFLKAANAMFRD"
     gene            2189..3136
                     /locus_tag="AC16CX_00835"
     CDS             2189..3136
                     /locus_tag="AC16CX_00835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011088.1"
                     /translation="MRSALWATAIATALLASAPAAFAQTFPGKLVRFVIPFPVGGSSD
                     ANARIIGPPLSERWKQQVIIDPRPGAATVVGTDHVAKSAPDGHTLLLTSTQYPQSPAL
                     FGKLPYDPYSDLVPVTLVTLSPYAIVGHPSLPAKNIKELIALARARPGELNMGNAGNM
                     LPTHLFNMLAKVKIENVPYKGAGPLMIDAMGGHVPLAIGAVSSVQAAVRSGRVRMLGV
                     TSPSAAFPDAPVIAKDVPGFDADTWFGLFAPRGTPRELVNRIRDDVAAVLQIPDVRQR
                     LLDIGGNPGGQTPEEFAARVRSEIEKWKKVAAAAGIKPQ"
     gene            complement(3195..>4777)
                     /locus_tag="AC16CX_00840"
     CDS             complement(3195..>4777)
                     /locus_tag="AC16CX_00840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3011089.1"
                     /translation="YPFDDPPGTPERSEPRYATFADHEARIPMRDGIRLAADVLRPSA
                     AGMKFPALVSTSVYTRQLQRGVVPLGQNEAGISEFWVPRGYAHVVVDVRGCNDSEGVY
                     DLFGPQEQQDLYDIIEWVSGQPWCDGNVGMSGISYFGRTQLFAAEQQPPHLKAIFPYD
                     ASCDFYRDACFHGGIATGFTTHWTAFVMNLNMTSGRNPNVDGIRQKLQIMYSQKYPFD
                     GPFYHERSAGLRLARVKIPAYFGSGWYMNELHLKGAFDGYLGTEGIPKRMMVGPRPWP
                     LRPWAGYHHEMLRWYDHWLKGMDTRVMEGPPIQLYIQGDNRWRSEHEWPLKRTQWREF
                     YLGGLPGGRQGTLMEAAGGEQVRRFEFDPASHDAFHGEPRLTYRTEPLARELELTGPL
                     ALYLQAASSAPDIDWLVFMADEAPDGQVRELCKGWLRASHRKIDPARSTPAKPFHPHL
                     AAEPLEPGAVYELPIEIWPICNVFKPGHRIRLEIANADSIIASQGRPHVTIRDKATNA
                     VYEGGRKPSRLVVPVIPR"
BASE COUNT          876 a         1549 c         1483 g          869 t
ORIGIN      
        1 gatgattcgg atacccgtgc cgcgtacttt ccccgcacaa gagtaccgaa gtcagagcgc
       61 ttatactccg gccggagttc gtccacaggt ttccgactag ccttcttcat aaatcgttct
      121 ttcacccgcc gtcgccaggc gtgcgctgat aattcggatt atatcgccgt gttcggtatg
      181 agcgacaacg aggagacgcc cgctggtgga gaacccgaaa gtaataaagc gctcttcgcc
      241 aactgaatga tcgggatccg ggcccgtcgc ggatagggca tcgcggaata ccgtggccgc
      301 ttcctcgaat gagaccccat gtttcgcgag attttccgcg gctttcgccg gatcccactc
      361 aaactgcaat tacagctccc tgattgcggt cgattcgcag gggggcgctc acccgtagcg
      421 gcgaagctgc tcccgtagct cgtgccagga gaccacctgg ccgcgatcgg cctgggcgat
      481 ggcctcaaga agcgcgcatt cctcctcggg ggatacctcg aaggtctcgt cttcctccct
      541 tgcgacaacc gtgacaacgg acccttcggc caaaggctcg ccttcaagga cgaccttgcc
      601 ccctatgact cttcccgttg cgattttcat acttgaattc tacacccccg gcgcgggccg
      661 tcgcttgtct tcccaggtcg ggagatcgaa aaaagctcgg tttcgccctc acgctcttac
      721 gctctcacgc cctcacggaa cttgttgttc tcccgctctc acgctctcac ggaatttgtc
      781 gctctcccgc tcttacgccc tcccgctctc acggatcctc tcgctctccc gctctcacgc
      841 cctcccgctc tcacgcggcg gcggttacta tagtctcctg cctgtcttaa tgcgctgagg
      901 ggagatgcat cgtggacgac atgttcgtat tgaatgcaga tcagaaggcg atcctggata
      961 cggtgcgccg ctacgttgac caggaggtga ggccgcgctc cgccatgctt gacgccaggc
     1021 aaaacccgga ggactgtttc tcgtgggaac tcgtaaagaa ggcgcacgag ctcggcatcc
     1081 gcaccatgac cctcgcggaa aagtggggcg ggctcgggct cgactcgctc accacggcaa
     1141 tggtggtcga ggagcttgcc gtcggcgaca tgggcgtcgc ggtcgtcatg gcccagacct
     1201 tcaagattgc ccagctcatg cagcagacgc tcaatgaaga acaacaaaag cgggtgctgc
     1261 ccgcctttgc gagggacccc cggggcgtgc tcgccatcgg catgtcggaa ccgggcaacg
     1321 gctcggacaa gcacacgaac cagccggtgc cgtttcaaac cggtgcggag cgaacgcgcg
     1381 ggggctggat cgtgaacggc atgaagcact tcatctccaa cggcaaccgg gcaagccatt
     1441 acctggtgat ggtgcagacc gaccggcaga agaccatggc gaacggctcg acgtgcttcc
     1501 tgctggagcg cgggcatccc ggattcatga tcggacgcat ccacgacaaa atgggcgagc
     1561 ggctcgcgaa caatgccgag ctcatctttc aggactgcac cattcccgac gggaacgtgg
     1621 tcggcgaggt caacaagggc tcggagatat tcgcgcgctt ctcgccgcag tccaacgcct
     1681 atgccggcgc cacggtgctc ggggtcgcgg tggcgctgca ccgcaaggcc atcgagtggg
     1741 cgaagctccg ggtgcagggc ggcaagcggc tcgtcgagca cgacagcatc cgcgcgcagc
     1801 ttgcggaaat gcgcatgctg atcgacgcct cgcgctcata catccatcgc gcctgctggt
     1861 tcgccgaccg cccgcagcac ggctgggaca agacactcgg tgcgctgccc aaggcgatgg
     1921 cggcgcaggc ggcgtggaaa atcgcgacct ggacgatgga gatccacggc ggccacggct
     1981 acatgaagga agcgggagtg gagaaactgg tgcgggatgc ggccgcgttc ctgcattcgg
     2041 atggcgtcaa ccggacgctc tttctgaagg cggcgaacgc aatgttccgc gactgacctg
     2101 cgcgttgacc tttcgcgcaa ggtggcctag cctatatttg atcctgccat cccccaatct
     2161 tcagattaga gcatcgtcga agcgagccat gcgttcggca ctttgggcaa cggccatcgc
     2221 aaccgcgctg ctcgcgtctg ctcccgccgc cttcgcgcag acgttccccg gcaagctggt
     2281 tcgcttcgtc atcccgttcc cggtgggcgg cagcagcgac gccaacgcgc gcatcatcgg
     2341 cccgccgctt tccgagcgct ggaaacagca ggtgataatt gacccgcgtc cgggcgcggc
     2401 cacggtcgtc ggcacggacc acgtggcgaa gagcgcacct gacggacata cgctgctgct
     2461 cacttccacg caatatccgc agagtcctgc cctgttcggg aagctgccgt atgatcctta
     2521 ctccgatctc gtgccggtca cgctcgtaac gctctcgccg tatgcgatcg tgggccaccc
     2581 gtcactgccc gcgaagaaca tcaaggagct gatcgcgctc gcccgggccc gtccgggcga
     2641 actcaacatg ggcaacgccg gaaatatgct tcccacgcat ctctttaaca tgctcgccaa
     2701 ggtgaagatc gaaaacgtgc cgtacaaggg cgcggggccg ctcatgatcg acgcgatggg
     2761 cgggcacgtg ccgctcgcga tcggcgcggt gagctcggtg caagcggcgg tgcgctctgg
     2821 ccgcgtgcgg atgcttggcg tcacttcacc atcagcagcg ttccccgacg cacccgtcat
     2881 cgccaaagac gtgcccggat tcgatgccga tacgtggttc ggcttgttcg cgccccgcgg
     2941 cacgccgagg gaactcgtca atcggatacg cgacgatgtc gccgcagtgc tgcagattcc
     3001 ggacgtgagg cagcggctgc ttgacatcgg cggcaatccg ggcggccaga ccccggaaga
     3061 attcgccgca cgcgttcgca gcgaaatcga gaagtggaaa aaagtcgccg ctgcggccgg
     3121 catcaagccg cagtaggcgc cgcgaatccg cggttacccg cggttggcgt gtcttgcacc
     3181 tgttactcgc gcggctaccg gggaatcacc ggcaccacga gccgcgacgg cttgcgtcca
     3241 ccctcataaa ctgcgttcgt cgctttgtcc cggatcgtca cgtgaggtct gccctggctg
     3301 gcgatgatcg aatcggcgtt ggcgatctcc aggcgaatgc gatgccccgg tttgaacacg
     3361 ttgcagatcg gccagatctc gatcgggagc tcgtacaccg cgcctggttc gagcggctcg
     3421 gcagcgagat ggggatggaa cggcttcgcc ggagtggagc gcgcgggatc gatttttcga
     3481 tgtgaagccc gcaaccagcc cttgcagagt tcgcgcacct ggccatccgg cgcttcatcc
     3541 gccatgaaga ccagccagtc gatgtcgggc gccgacgaag ccgcctggag gtaaagcgcg
     3601 agcgggccgg tcagctccag ctcccttgcc agcggttccg tccggtaggt aagccgcggc
     3661 tcgccatgga atgcgtcgtg actggccgga tcgaattcga aacgccggac ctgctcgccc
     3721 ccggccgcct ccatcagggt gccttgccgg ccacccggca ggccgcctag gtaaaactcg
     3781 cgccattgcg tccgcttgag cggccactcg tgctccgagc gccacctgtt gtcgccctgg
     3841 atataaagct ggatgggcgg cccttccatg acccgcgtgt ccatgccctt cagccaatgg
     3901 tcgtaccagc gcagcatctc gtggtgatag cccgcccagg gacgcagcgg ccagggtcgc
     3961 gggcccacca tcatgcgctt gggaatcccc tcggtcccga ggtagccatc gaacgcgccc
     4021 ttgaggtgca gctcattcat gtaccacccg gagccgaagt aagcgggaat cttcaccctg
     4081 gcgagccgca gcccggccga acgttcgtga tagaacggcc cgtcgaacgg gtacttctgg
     4141 ctgtacatga tttgaagctt ctggcggatg ccatcgacgt tcggattacg gccggaagtc
     4201 atgttgagat tcatgacaaa ggcggtccaa tgggtcgtga agccggtcgc gatcccgccg
     4261 tggaagcagg catcccgata gaagtcgcac gaggcatcgt acggaaagat cgccttcagg
     4321 tgcggcggtt gctgctcggc cgcgaaaagc tgggtgcggc cgaaatagga gatgccgctc
     4381 atgccgacgt tgccgtcgca ccacggctgc ccggagaccc attcgatgat gtcgtacagg
     4441 tcctgctgct cctgggggcc gaagaggtcg tacacgcctt ccgaatcgtt acaaccgcgg
     4501 acgtcgacga cgacatgggc gtagccccga ggcacccaga actccgagat gccagcctcg
     4561 ttctggccga gaggcaccac gcctctctgg agctggcgcg tgtaaacgga ggtcgaaacc
     4621 agggccggaa acttcattcc cgcggcagag ggccgaagca cgtcggcggc cagcctgatc
     4681 ccgtcccgca tcggaatgcg ggcctcatgg tccgcaaatg tcgcgtagcg gggctcggat
     4741 cgctcgggtg tccctggcgg gtcgtcgaag gggtatt
//
LOCUS       DBNGWZ010000031         2915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_254346, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000031 DBNGWZ010000000
VERSION     DBNGWZ010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_254346"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..340)
                     /gene="glnK"
                     /locus_tag="AC16CX_00845"
     CDS             complement(2..340)
                     /gene="glnK"
                     /locus_tag="AC16CX_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3011090.1"
                     /translation="MKLVTAIIKPFKLDEVREALSAVGVTGVTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAIKSEMLEQVIEAIEKAANTGKIGDGKVFVFDLEQAIRI
                     RTGETGADAL"
     gene            complement(360..1187)
                     /locus_tag="AC16CX_00850"
     CDS             complement(360..1187)
                     /locus_tag="AC16CX_00850"
                     /inference="COORDINATES: protein motif:HMM:NF021230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HIR3011091.1"
                     /translation="MFKKAILAGLTAAALGSGVAQAQQQPASPHTLTGNVGLYSQYIF
                     RGLTQTNREPALQGGFDYSHASGFYAGTWGSNISWLSDSGAYMAGGSLEWDFYGGYKW
                     SLPSDLTLDLGTLYYWYPGDANTVANPLNPKANTWEVYAGLSWKWLSGKLSYSIDNKT
                     FGVLDSSGTYYLDLTAAYPVPDTKLTLIAHYGVQKFKGQDPRLIGVASNDNACSYEDW
                     KLGLSYALPKDFTVGAFYTDTSGANSLCYGTPFETPAGAYPKNISKGTGTVFISKTF"
     gene            1488..1745
                     /locus_tag="AC16CX_00855"
     CDS             1488..1745
                     /locus_tag="AC16CX_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR3011092.1"
                     /translation="MANSKLFEEIDQKMREILARSPAADLEKNLRVLLQSAFSRLDLV
                     TREEFDVQREVLARTRAKLQELETKLAELEGKSEVESRKSK"
     gene            1875..>2915
                     /locus_tag="AC16CX_00860"
     CDS             1875..>2915
                     /locus_tag="AC16CX_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR3011093.1"
                     /translation="MSLAVVFSRALAGMEAPPVTVEAHLANGLPSFTIVGLPEAEVKE
                     AKDRVRAALQNARFEFPARRITVNLAPADLPKESGRFDLPIALGILAASEQLPAGRLA
                     GCEFAGELALTGELRPIRGALAMALSAVRAGRAFVLPEANAREAALVEEATIYPAYSL
                     LDVCAHLTGRIPLERCHLQPVLNGCRHPDMLEVKGQLHAKRALEIAAAGGHSLIMVGP
                     PGTGKSMLAERLPGILPPMTREEALAAAAMQSLGSSGFDLANWARRPFRAPHHTASAV
                     ALVGGGSHPRPGEISMALHGVLFLDELPEFDRKVLEVLREPLESGRISVSRAARQAEF
                     PAEFQLVAAMNPC"
BASE COUNT          501 a          934 c          935 g          545 t
ORIGIN      
        1 cctatagcgc atcggccccg gtctcgccgg tgcggatgcg gatggcctgc tctagatcga
       61 agacgaacac cttgccgtcg ccgatcttgc cggtattggc ggctttctcg atcgcctcga
      121 tcacctgttc gagcatttcg ctcttgatcg cggcttcgat cttgaccttg ggcaggaagt
      181 ccaccacgta ttcggcgccg cggtagagct cggtgtgccc cttctgccgg ccgaagccct
      241 tgacctcggt gacggtgacg ccggtcacgc cgacggcgga cagcgcttcc cgcacttcgt
      301 caagcttgaa cggcttgatg attgctgtga cgagtttcat tcggtctccc cgtgtgtcgt
      361 tagaaagtct tcgagatgaa aacggtgccg gtacccttgg agatgttctt cgggtaagcg
      421 cccgccggcg tctcgaacgg cgtgccgtag cacaggctgt tcgcgccgct cgtatcggtg
      481 tagaaggccc cgaccgtgaa atccttgggc agcgcgtagg agagcccgag cttccagtcc
      541 tcgtacgagc atgcgttgtc gttgctggct acgccgatga gccgaggatc ctggcccttg
      601 aacttctgga cgccatagtg cgcgatgagg gtgagcttcg tgtcgggaac cgggtacgcc
      661 gccgtcagat cgaggtagta cgtgccgctc gaatcgagca cgccgaaggt cttgttgtcg
      721 atgctgtagg agagcttgcc cgagagccac ttccagctca acccggcgta aacctcccat
      781 gtgttggcct tgggattgag cgggttcgcg acggtgttgg cgtcacccgg gtaccagtag
      841 tagagcgttc ccagatcgag cgtgagatca ctgggcaggc tccacttgta gccgccgtag
      901 aaatcccact ccaggctgcc gccggccatg taagcgccgc tgtcgctcaa ccagctgatg
      961 ttggagcccc aggtgccggc gtagaaaccg cttgcatggc tgtagtcgaa tccgccctgc
     1021 aatgccggct cgcgattggt ctgggtcaag ccgcggaaga tgtactggct gtacagcccg
     1081 acgttacccg taagcgtgtg tggagaagcg ggctgctgtt gtgcctgggc gactccggat
     1141 ccgagcgccg cggcggtgag cccagcgagg attgctttct tgaacatggt tacccctttc
     1201 gttattaacg aatacgcaat attgcaaagc tgtgctgagc acagatcgtg ccaaccaact
     1261 tgtgccttta aaacaactgg atatgaaaac ggcgcggggg acgcgaacac acgtcagcac
     1321 cgctttagtg cgtagaaaag cccggttgca ccaaatccga gcggggtaaa ttcagcccgg
     1381 gctcggaagg cagcccgccc cacccaattc atcctcgcaa cgctggacgc ctatcgtcgt
     1441 ctccgttgta ggaaaatcgg acagcgcctc actgaaccga gggccgtatg gccaacagca
     1501 aattgttcga ggaaatcgac cagaaaatga gggagatact cgcccgcagc ccggcggcgg
     1561 acctcgagaa gaacctgcgc gtcctgttgc agtccgcctt cagccgcctc gacctggtga
     1621 cgcgcgagga attcgacgtg cagcgcgagg tgctcgcgcg cacccgcgcc aagctccagg
     1681 aactcgagac gaagctcgcc gagcttgaag gcaagtcgga agtcgaaagt cgaaagtcga
     1741 aataaatcct gccgtaacaa cggtttttct tccgactttc gcctttcgac tttcaccttg
     1801 cttctccgcg tcgtccaccg cgcccactcg tccgttaatg gcggtatcaa ctgctgcctg
     1861 gagggggtga gtcgatgtcg ctcgccgtcg tattcagccg cgcgctggcg gggatggaag
     1921 cgccgccggt cacggtggag gcacacctcg ccaacggctt gccgagcttc actatcgtgg
     1981 ggctccccga agccgaggtg aaggaggcca aggaccgcgt gcgcgcggcg ctgcagaacg
     2041 cgcgctttga gtttcccgcg cggcgcatca ccgtcaacct cgcgcccgcc gacctgccca
     2101 aggagtcggg ccgcttcgac ctgccgatcg cgctcggcat cctcgccgcc tcggagcagt
     2161 tgcccgccgg gcggctcgcc ggctgcgagt tcgccggtga gctcgcgttg accggggagt
     2221 tgcggccgat ccgcggcgcg cttgcgatgg cgctttcggc ggtcagggca gggcgcgcct
     2281 ttgtgcttcc ggaagccaac gcgcgcgagg cggctttggt cgaggaggcg accatctacc
     2341 cggcgtattc cctgctcgac gtgtgcgccc atctcacggg gcgcattccg ctcgagcgct
     2401 gccacctaca gcccgtcctg aacggctgcc gccatcccga catgctggag gtcaaagggc
     2461 agctgcacgc caaacgggcg ctcgagatcg cggccgcggg cgggcacagc ctcatcatgg
     2521 tcggcccgcc gggcaccggc aagtccatgc tcgcggaacg gctgcccggg atcctgccgc
     2581 cgatgacgcg ggaggaggcg ctcgccgcgg cggccatgca atcgctcgga agcagcggct
     2641 tcgacctcgc gaactgggcg agacgcccgt ttcgcgcgcc gcaccacacc gcgtccgcgg
     2701 tggcgctggt gggcggcggc agccatccgc gacccggcga aatctcgatg gcgctgcacg
     2761 gggtgctgtt cctcgatgag ctgccggaat tcgaccgcaa ggtgctcgaa gtgctgcgcg
     2821 agccgctcga atcaggccgg atcagcgtct cccgcgccgc gcgccaggcc gaattccccg
     2881 ccgagtttca gctggtcgcg gcgatgaatc cctgc
//
LOCUS       DBNGWZ010000032         4013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1010390, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000032 DBNGWZ010000000
VERSION     DBNGWZ010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1010390"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="AC16CX_00865"
     CDS             complement(<1..947)
                     /locus_tag="AC16CX_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3011094.1"
                     /translation="MDSVDLQVLRSASDWVKAGRGVVMATVVHTWGSSPRPIGALTAI
                     RDDGMVVGSVSGGCIEDDMILQVREGKLVQDKPATTQYGVTAEEAKRFGLPCGGTLEL
                     VLEPLTKESGLDELLARVERHELVMRRLDMKSGRVTIGPAKSTDLLTFDGTALVTVHG
                     PRWRLLIIGAGQLSTFLAQMAQGLDYQVTVCDPREEYADTWEVPAVDLKRGMPDDVVT
                     GMNLDGHSAVVTLTHDPKLDDMALLEALKSPAFYVGAIGSRKNNDARRRRLAEFDLSQ
                     EEIARLHGPVGLKLGAKTPPEIAIAILAEMTAIRNGVKVP"
     gene            1202..1675
                     /locus_tag="AC16CX_00870"
     CDS             1202..1675
                     /locus_tag="AC16CX_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3011095.1"
                     /translation="MGISISMTVNGKPVSADLDPRTLLVQFLREQLRLTGTHVGCDTG
                     QCGACTVHVNGRSVKSCNVLAAQMQGAQVVTIEGISHNGELHPMQAAFRDHHGLQCGF
                     CTPGMVMSAIELVKIHPDPSEETVRAGLEGNYCRCTGYHNIVKAVLAGAAAMRQR"
     gene            1771..>4013
                     /locus_tag="AC16CX_00875"
     CDS             1771..>4013
                     /locus_tag="AC16CX_00875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3011096.1"
                     /translation="MSAPLIGTSVKRKEDYRFLTGGGQYTDDVALPGQAHAFFLRSPH
                     AHAKIRSMDLERARSAPGVIAVFTGDDLAKAKVGGLPCGWLITGVDGQPMKEPPHPPL
                     AQAKTRYVGDHVAVVIAETLNQAKDAAELIDVDYEVLPAVVKAADARKPGAPVLHDIA
                     PDNTCYVWALGDKSAVDKAFTSAHHVTTLEFVNNRLIPNAIEPRAAMASYSRAEDAYT
                     LYVTTQNPHVERLLMTAFVLGLPEHKVRVIAPDVGGGFGSKIYLYAEETVITWASKQV
                     NRPIKWTAERSESFLSDAHGRDHVTKAELALDKSGKFLAMRVTTTANLGAYLSTFASC
                     IPTILYATLLAGQYTTPAIHCEVTAVFTNTTPVDAYRGAGRPEATYVVERLVDTAARE
                     TGIDRAEIRRRNFIKEFPYQTPVALLYDTGNYDATLTEAMKLADVAGFEARRKDAEKR
                     GRLRGLGYATYIEACGIAPSNVAGSLGARAGLYEAGEIRVHPTGTVTVFTGSHAHGQG
                     HETTFAQVVADRLGLPIENVDIVHGDTGRVLFGMGTYGSRSIAVGGTAIVRALDKIVA
                     KGKKIAAHLLEASEADIEYESGTFKVAGTDRKKTFAEVAFAAYVPHNYPLDRLEPGLN
                     ESAFYDPTNFTFPAGSHICEVEIDPDTGQTKIVGFTAVDDFGKVINPMIVEGQVHGGL
                     AQGIGQALLEGCAYDPESGQLLTGTYMDYALPRADDLPSFRVGTKETPCTHNPLGVKG
                     CGEAGAIG"
BASE COUNT          719 a         1359 c         1279 g          656 t
ORIGIN      
        1 ggaaccttca cgccgttgcg gatcgcagtc atttccgcga ggatcgcgat cgcgatctcg
       61 ggcggcgtct tggcccccag cttcagccct accggcccgt gcagacgcgc gatctcctcc
      121 tgcgagaggt cgaattcggc gagtcgccgg cggcgtgcat cgttgttttt cctggacccg
      181 atcgcgccca catagaaagc cggcgacttg agcgcttcga gcagggccat gtcgtcaagc
      241 ttgggatcgt gggtcagcgt caccaccgcg ctgtggccgt cgaggttcat tccagtcacc
      301 acgtcatcgg gcatgccgcg cttcaaatcc acagccggca cttcccaggt atcggcgtat
      361 tcctcgcgcg ggtcgcacac cgtgacctgg tagtcgagcc cctgcgccat ctgcgcgagg
      421 aacgtcgaaa gctgtccggc gccgatgatc agcagccgcc agcgcggacc gtgcacggtg
      481 acgagcgccg tgccgtcgaa agtcagcagg tccgtggatt tcgccgggcc gatcgtcacg
      541 cgcccggatt tcatgtcgag ccggcgcatc acgagctcgt ggcgctcgac ccgcgcgagc
      601 agctcgtcga ggccgctttc cttcgtgagc ggctcgagca ccagctcgag cgtccccccg
      661 cagggcaggc cgaagcgttt cgcttcttcc gccgtgacgc cgtactgggt cgtggcgggc
      721 ttgtcctgga cgagcttgcc ctcgcgcacc tgcaggatca tgtcgtcctc gatgcaaccg
      781 ccggagaccg accccaccac catgccgtcg tcgcggatcg cggtaagcgc cccgatcggc
      841 cgcggggatg agccccacgt gtggacgacg gtcgccatca ccacgccccg gccggccttg
      901 acccagtcgc tcgcgcttct caaaacctgc agatctacgc tgtccatacc cggtttccgg
      961 tcagcttcag ggagggctat attctactaa atataacggt attgcgcacg cacgcatgga
     1021 aaccgggtta ccgcgtgaag cgacaatccg gatccgggct caacggttca catttgggcg
     1081 gttgcaaggg ccgtttacgt gagctagcat ccccggaaat tcttccccga ttctcttcga
     1141 cgatgcggtg gcagcgacga cccgtccggg caccgcggcg cgtcaccatc tggaggattt
     1201 catgggcatt tccatctcga tgaccgtcaa cggaaaaccc gtcagcgccg atctcgatcc
     1261 ccggacgttg ctggttcagt tcctgcgcga gcagcttcgg ctcaccggca cccacgtcgg
     1321 ctgcgacacc gggcagtgcg gcgcctgcac cgtgcacgtg aacggccgct ccgtcaagtc
     1381 gtgcaacgtg ctggccgcgc agatgcaggg cgcgcaggtg gtgacaatcg agggcatcag
     1441 ccacaacggc gagctgcacc cgatgcaggc cgctttccgt gaccatcacg gactgcagtg
     1501 cggcttttgc acgccgggca tggtcatgag cgcgatcgag ctcgtgaaaa tacaccccga
     1561 tccctcggaa gaaacggtcc gcgccgggct ggagggtaat tactgccgct gcaccggcta
     1621 tcacaacatc gtcaaggcgg tgctggccgg cgctgctgcg atgagacagc ggtaagcggt
     1681 aagcgggaaa ttgggctcgc gttctcggga tgttccccgc tcgtagctca ctactcatcg
     1741 ctcacggctc acggctttta ggaggacaca atgagcgcgc cattgatcgg cacttcggtg
     1801 aaacgcaagg aggactaccg cttcctgacc ggcggcggcc agtacaccga cgacgtcgcg
     1861 ctgcccggac aggcgcacgc ctttttcctg cgctcgccgc acgcgcacgc gaagatccgc
     1921 agcatggacc tcgagcgggc gcggtccgcg ccgggcgtga tcgccgtgtt caccggtgac
     1981 gatctcgcga aagccaaggt cggcgggctt ccctgcggct ggctcattac cggagttgac
     2041 ggccagccga tgaaggagcc accgcacccg ccgctcgcgc aggccaagac gcgttacgtg
     2101 ggcgatcacg ttgcggtcgt gatcgcggag acgctcaacc aggcaaagga cgcggccgag
     2161 ctgatcgacg tggactacga ggtattgccg gcagtcgtga aagcggccga cgcgcgcaag
     2221 cccggcgcgc cagtattgca cgatatcgcg cccgacaaca cttgttatgt atgggcgctt
     2281 ggcgacaagt cggctgtgga caaagcgttc acatccgcgc atcacgtcac cacgctcgag
     2341 ttcgtcaata accgcctgat cccaaacgcc atcgagcccc gcgcggcgat ggcgagctat
     2401 tcgcgcgccg aggacgccta cacgctctac gtgacgaccc agaacccgca cgtcgagcgg
     2461 ttgctgatga cggcgttcgt gctggggctg cccgagcaca aggtgcgcgt gatcgcgccc
     2521 gacgtcggtg gaggtttcgg ctccaagatc tatctctacg ccgaggagac ggtgatcact
     2581 tgggcctcga aacaggtgaa ccggccgatc aagtggaccg ccgagcgctc ggaatccttc
     2641 ctctcggacg cgcacgggcg cgaccacgtg accaaggccg agctggcgct ggacaagagc
     2701 ggcaagttcc tcgcgatgcg cgtgaccacc accgcgaacc tcggggccta tctttcgaca
     2761 ttcgcttcct gcataccaac catcctctac gcgacgctgc tcgccgggca gtacaccact
     2821 ccggcgatcc attgcgaggt gaccgcggtc ttcaccaaca ccacgccggt ggacgcttac
     2881 cgcggcgcgg gacggcccga ggccacttac gtcgtcgagc gcctggtgga taccgcggcg
     2941 cgcgagacgg ggatcgaccg cgccgagatc cggcggcgca atttcatcaa ggagttcccg
     3001 taccagacgc cggtcgcgct gctctacgac acgggcaact acgacgcgac gctcaccgag
     3061 gcgatgaagc tcgccgacgt ggccggcttc gaggcgcgcc gcaaggacgc ggagaagcgc
     3121 ggcaggctgc gcggcctcgg ctacgccacc tacatcgagg cctgcggcat cgccccatcc
     3181 aacgtcgcgg gatcgctcgg cgcgcgcgcg gggctgtacg aggcgggcga gatccgcgtg
     3241 catcccaccg gcaccgtgac ggtattcacc ggaagccacg cccacggcca gggacacgaa
     3301 accacgttcg cgcaggtggt cgccgaccgg ctcgggctcc cgatcgagaa cgtggacatc
     3361 gtgcacgggg acaccggccg ggtccttttc ggcatgggca cctacggctc gcgctcgatc
     3421 gcggtcggcg gcaccgcgat cgtgcgggcg ctcgacaaga tcgtggccaa aggcaagaaa
     3481 atcgccgcgc acctgctcga agcgtcggaa gccgacatcg agtacgagag cggcaccttc
     3541 aaggtcgccg gcaccgacag gaagaagact ttcgcggagg tcgccttcgc ggcgtacgtg
     3601 ccgcacaact acccgctgga ccgtctcgag cccgggctga acgagtccgc tttctacgat
     3661 ccgaccaatt tcacgttccc ggccggcagc cacatctgcg aagtcgagat cgatcccgac
     3721 accggccaga cgaagatcgt cggcttcacc gccgtggacg acttcggcaa ggtcatcaac
     3781 ccgatgatcg tcgaggggca ggtacacggc gggctcgcgc aaggcatcgg ccaggcgctg
     3841 ctcgaaggct gcgcctatga cccggagagc gggcagcttc tcaccggcac ctacatggat
     3901 tacgcgctgc cgcgggccga tgacctcccc tctttcaggg tcgggaccaa ggaaacgccg
     3961 tgcacgcaca atcctctggg cgtcaagggc tgcggcgaag cgggcgcgat cgg
//
LOCUS       DBNGWZ010000033        19349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_758796, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000033 DBNGWZ010000000
VERSION     DBNGWZ010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_758796"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16CX_00880"
     CDS             <1..212
                     /locus_tag="AC16CX_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011097.1"
                     /translation="VAPAGTPPAIIQRLSAEVIKAMKSPAIRERIEGQAFIPVFDTPE
                     AFAASLKKERQMWADVIRRNNITAE"
     gene            complement(564..1394)
                     /locus_tag="AC16CX_00885"
     CDS             complement(564..1394)
                     /locus_tag="AC16CX_00885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3011098.1"
                     /translation="MTQAGWLWPGDKRIAVVFNVCLEAWSDGKAPGISPMGNPLPPGV
                     LDNMAISWAAYGAKRGIYRLLDAFARHGARASVMVSAILAERTPEAVKAVGDAGHEIL
                     SHSYAMDVIPALMTEGEERKNIQRCTALLEKVSGKRVKGWLSPRGTPSANTARLLAEA
                     GYTWYGDVFDDDLPYVQTFGKRKIVAIPLSTDVNDMPSMKYGNPPRMMLETFEEQLKR
                     IANRREGPVIIDVTSHAHIFGRPHGAYYYEKIVQTAASKRDIWIATRLDIAEFILARE
                     "
     gene            complement(1427..2395)
                     /locus_tag="AC16CX_00890"
     CDS             complement(1427..2395)
                     /locus_tag="AC16CX_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011099.1"
                     /translation="MVKSAVRMIMFGACVLCVAPGWAQGYPAKPVRVIVGFSAGSTTD
                     LIGRVLASKMGDGLGQPVVVDNRPGAGANIAAELVSKAPPDGYTVLLANAGIATGATA
                     YVKLNFNALRDFAPVTQVSATPHILVTHPSLPPKTVKDLVAFTRARPGELNFASTGHG
                     NSDHLAGELFSYMTGLKMVHVSYKGGPLALNDVITGQVAMYFAGVPVALPLVKAGRVR
                     ALGVTGAKRAAVLPEVPTVAEAGVPGYEHILWGMLLVPAATPKDVVARLNREAVKVLE
                     AADVRERYAGMGVEPAPSTPERAAAYLKSETEKYAKVVKAIGLRIE"
     gene            2669..3655
                     /locus_tag="AC16CX_00895"
     CDS             2669..3655
                     /locus_tag="AC16CX_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011100.1"
                     /translation="MKQALLQSLLVLAAAAVSAGAGAQATSKEPGYPTKPIRVVVPFT
                     PGSATDIIARIIGPKLVERWGRPVVMDNRPSAGGVVAFSIVAEATPDGHTLLVTGSNF
                     AGSAALYSKLPYDPVGDFSGISQFASTPLVLVVAPALGAKSVRELVALAREKQGQLNY
                     GSTGLGSGPHYGAELFKLASGINAVHVPYRGSPESLTDLMAGRVHFVLSPVLAVSPLI
                     KSGRLLALGVTTTYRAQALPDVPTIAEAALPGFEYQGWYGMLAPSRTPRAIINRLAKE
                     VAHILELPEIKERIASQGAIARSSSPEAFDKLVHNEIATRRKVWKAAGVKVE"
     gene            3772..4950
                     /locus_tag="AC16CX_00900"
     CDS             3772..4950
                     /locus_tag="AC16CX_00900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011101.1"
                     /translation="MDFSLNEVQRGWQQKAREFAREEIRPLSLERDQVEGGFAPWDWD
                     IIRKGSQLGFRTLAVPEEWGGPGADFVTQGLVMAELASGDSAMSKAFSQNWKWSHQIA
                     EFCTREQKERFLKPFLADDTYLMGSASTEPNAGSDNRYPPDDDARAGYRLRAVRQGEV
                     WILNGEKTFIANGSVGKLFFVNTRTDPNAPIRDGTTTFLVPIDTPGVRIGKIFNKNGW
                     RFYQNAELVFEDARVPHANVVGEVNGGMKVRSGAAAEFGDLELAANALGVCDAACESA
                     MRRAKGERRGGKLLYDHQLVQLKINEMFALTESLRSFVLRTAWEMDHRVKSANPILAM
                     NHSTRVIQRVTRLNLELHASSEGAVDAMAHKLVRDAIIWSHLAGDNTQQLKAVRRLTK
                     "
     gene            complement(5191..6210)
                     /locus_tag="AC16CX_00905"
     CDS             complement(5191..6210)
                     /locus_tag="AC16CX_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011102.1"
                     /translation="MVAAGFRRFLTTGALRVALCASAVFLGAAPCIAKQSEVLAVYPV
                     KPIRIIVASSPGAADDFFARSLGDELEAFYRQKVVVDNRSGAGGLIGNTVVSKANADG
                     YTLAMIGVTRLITELLREQPPYRALADIVGVAHVASITNVLAVTPALPVRTAREFAAY
                     ARPRFGELNYASLGIGSASHLAGEVFTRALGIDAMHVPFRNVSDSFVEMLLGRVHYAV
                     LTLPTVLGPVREGRLRVLAVMTPQRSPALPAVPAIAELGLPEAQFDRWSGIVAPRGTP
                     RRIVEQLHGDIVRGLRKSALRELFVRQGAESTPESTPDGFMRLMQEEYLRYQALIREG
                     GIRPE"
     gene            complement(6237..7331)
                     /locus_tag="AC16CX_00910"
     CDS             complement(6237..7331)
                     /locus_tag="AC16CX_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011103.1"
                     /translation="MKAQNLARDPPARASTLLAWRRGMVLLAGLSVGPPALAHVKWFS
                     EYDLNQPPLPIGDVLTGQFVHFFLASVLLIYVFFWLDRYVLRKRILEDRLRRYTVTEP
                     VAFGIMRGAAFVFLAAVSAYGFVVQGFFLTPELTTGQRWVPWMQAGMALCALDRRTVP
                     LIGVGLAGLFIAAAGRYGIFHLLDYLILLGVSYYFLAARLPGPGWLMSRYIVLYAGTA
                     LTLLWAAIEKWGYPGWTYPLLAREPDLLMGLEPSTYMVLAGFVEFDLAFMVLSGVSLL
                     ARATALGFAFVFALAIYKFGLIDAIGHLLIMAILLVLVIHGPTRGRNFLVLGNKSLWT
                     EAYFMTGLYTLAFVLVFIAYYGLRALAYGN"
     gene            complement(7448..7597)
                     /locus_tag="AC16CX_00915"
     CDS             complement(7448..7597)
                     /locus_tag="AC16CX_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011104.1"
                     /translation="MCYDYERLEQLRKELEKAEELKKQQSKTQTPAKPAAPGKRVEQE
                     EPVPV"
     gene            8081..8662
                     /locus_tag="AC16CX_00920"
     CDS             8081..8662
                     /locus_tag="AC16CX_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0016192 - vesicle-mediated transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HIR3011105.1"
                     /translation="MKLVERVKNILIQPKSEWAVIEQEQTDTKTLYTSYIAILALIPA
                     AATFISLLGGRIGTGLVLGAIVTQYLLSLVMVYAVAVIAEVLAPNFDGNRDSSGALKL
                     TTYAMTASWVAGVFAIAPFLGWLLSFLGSLYSIYLFFLGAPVLMKVPETKAVAYTLLV
                     MVAAIVIGAVIGFIGTGIMGFGSTGSMMRPKDF"
     gene            8807..9769
                     /locus_tag="AC16CX_00925"
     CDS             8807..9769
                     /locus_tag="AC16CX_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011106.1"
                     /translation="MNPGARLLCFLLLASAWGGASAQSYPIKPVRLIVASSAGSNPDV
                     LARIVAVGLTQALGQQVIVENRAGAGGNIGAEVAARAPADGYTVFLVHTNHPINTALY
                     PKLAYDLLTDFASITQLAMSAFVLVVHPSLPAKSVRDLVTLAKARPGAITFSSAGTGS
                     GTHLSAEYFNGMVKVKMLHVPYNGGGPALTAIASGETSVYFAPVSTGLPHILSNKLRP
                     LGVTAARRLRELPDVPTIAATVPGYEFTSWAGLMVPVKTPKHVIDTMHKAAIAALKNP
                     ETSKRLDTAGFVALGNRPEDLSAHIKSEIEKLAKLIRQIGLRPE"
     gene            9813..10922
                     /locus_tag="AC16CX_00930"
     CDS             9813..10922
                     /locus_tag="AC16CX_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3011107.1"
                     /translation="MNHKFPSAAVVTAAILLAGCAGQRAADTAEGPGARPRGVPVFEV
                     DPSWPKVPEKYKLGDASSIGIDAQDNVWVLHRPRTLKPDQARNAAPAILVFDPAGNFI
                     KTWGGAGSGFEWPEREHGIHIDHKGYVWLGGNNCPARNLPGLKPAADDQLLKFTQDGK
                     FVMQIGRANRSKGNADTLNLSQPADAWVHRKTNELFVADGYGNHRVAVFDADTGRFKR
                     MWGAFGNKPVDDYRCPPPSLKAVPDGPGPDQFSIVHAIRVANDGMVYVADRENRRVQM
                     FTSDGKFIKQFVRHNAPFARNLALSPDPEQRFLYVGGGIDIVVLERKTLEFVDVIGGP
                     GFLGGGHQIATDSKGNIYVAATTRGLQKLAFKGVR"
     gene            10935..11855
                     /locus_tag="AC16CX_00935"
     CDS             10935..11855
                     /locus_tag="AC16CX_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3011108.1"
                     /translation="MPSDPKSVTPAESPRVTRLRTLLGDYPTTRALRRGELSSSRVAF
                     DFADVPQVANAFKRTVRNLEFDVSELAIVTFLMAKAHGKPYVLLPAVVLGRFQHPFLV
                     YNSARGELRPQDLNGRRVGIRSYSVTTSMWIRGILANDHGVNIDSIRWVTFEEPHVAE
                     FRDPPSVERAPAGRELLSMLLDGEVDAAVLRDSNLTDPRLKRIIPDPDQAARDWHVRN
                     RAIQINHMVVVKESLSKSDPDAVREVYRLLREAKRLGPAPSTPDMTPIGLEANRRNLE
                     VAIDYVHQQRLIPRRYAVDELFDDVTRKLA"
     gene            11969..12757
                     /locus_tag="AC16CX_00940"
     CDS             11969..12757
                     /locus_tag="AC16CX_00940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3011109.1"
                     /translation="MHIYDPRFPQLASRSPVANAAVPDYRLLQKRIGTTRVVVVQPRN
                     HATENQVTLDAVAQLGPGARGIAVLHPTVTDAELKKLDAGGIRGIRFSLGDPATAVVT
                     IDMLEPLSKRVAALGWHVQLHMPGDMIAENADLLRRLPSQIVIDHMGRFPPAVGLAHP
                     AYQVILGLIEKGRTWVKLAGAYLNTRIGPPTYADATKIARAFVKAAPERLVWGSDWPH
                     PTEKNKPDDAQLFDLLAEWAMDRKARNRILVTNPEVLYGFAKTG"
     gene            12982..13395
                     /locus_tag="AC16CX_00945"
     CDS             12982..13395
                     /locus_tag="AC16CX_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011110.1"
                     /translation="MRTTKRRAKPGSVGRGRFFHIVVRPNSQFVRFRVQDIGARGGVE
                     RVAGQRSNGSWDTVKWLVEKTHAHVRGGTLVADTAEARKLLRSLGSRPVHLGGDRFRA
                     APRANIPESEKPTAGQLRAWKRNIRKAQQARRGGR"
     gene            complement(13488..14141)
                     /locus_tag="AC16CX_00950"
     CDS             complement(13488..14141)
                     /locus_tag="AC16CX_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3011111.1"
                     /translation="MPRILGLLFAAFLALTAAAQPPFPELESRAAALKALEDADARFR
                     LAGVVYFGRNGLAADGPLLVKRLSDENPVVREVAGQAVWLAWSRSGDAETDRLLETGA
                     AEMNAGRYPRAIEIFSKVIVRKPDFAEGWNKRATAYYLAGEMRKSLADCDEVMKRNPQ
                     HFGALSGYGQIYVRLEEYEKAIEYFWRALKVNPNLTDLETVIRGLEELVRERRRRSI"
     gene            14292..14798
                     /locus_tag="AC16CX_00955"
     CDS             14292..14798
                     /locus_tag="AC16CX_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HIR3011112.1"
                     /translation="MAVSMHPISLPVFVRHLTGLAGCLKKAQALYAEKKYDEATLVSY
                     RFYPDMFHFARQVQAATDHAKGCAALLAGLEAPKYEDNERSLADLIARVEKTIAYLNT
                     IQPGQIEGAEEKAVTVKMRDRELTYKGLDLLLKRSLPNFFFHVTTAYDILRHNGVEIG
                     KRDFMGPN"
     gene            14902..15792
                     /locus_tag="AC16CX_00960"
     CDS             14902..15792
                     /locus_tag="AC16CX_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3011113.1"
                     /translation="MERKKASDFPQEVLKLFDGYVHGGLTRRQFLDRAAKYAVGGFTA
                     AAMLEALRPNFAWGQQVATDDKRIRTEYLTYSSPQGSGTMKGYLARPAKVEGKLPGVL
                     VIHENRGLNPHIEDVTRRLAVVGYVAFAPDSLTPVGGYPGDEDKARDLFAKQDPKKRI
                     EDLVAGAYFLKSHPECNGRIGAVGFCFGGGICNVLAVRIPDLAASVPFYGIQVSAEDA
                     AKIKAPLQIHYAENDERINKGWPAYEAALKANGVRYQMYMYPGTQHAFHNDTTPRYDE
                     GAAKLAWSRTLAFFKEHLKG"
     gene            complement(15799..16761)
                     /locus_tag="AC16CX_00965"
     CDS             complement(15799..16761)
                     /locus_tag="AC16CX_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011114.1"
                     /translation="MTTMTLRGLIVLTIALAGIAPVHAQYPTRTIRFIVPFAAGSGSD
                     TVARFVQPQFSAALGQQVVIDNRAGAAGNLGAEVAAKAPPDGYTLLMANISHSISMTL
                     YSKLGFDIVRDFAPVSSLASGSFCLAVHPSVPAKSVKELIVFAKKRPGEINVATAGAA
                     IRLAAKMLDGMAGIKTTEVNYKSTPQAVTAVISGEASIGFPATSATLPHARAGKLRVL
                     AVTTPKRSSMAPDIPTLAEAGVPGYDVTTWYGLVVPAGTSKEIIARLHAATVSALAHP
                     AVKERFAPTDLEPYASTPEQLGAYIRSEVAKWAKVIKESGVRAD"
     gene            17012..18001
                     /locus_tag="AC16CX_00970"
     CDS             17012..18001
                     /locus_tag="AC16CX_00970"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011115.1"
                     /translation="MSQLKLSIATTDYDHFRDFRLGVVTAEGIELNWLNLGHHECFAR
                     FTANREFDLSELSFAKFAAQVTREDSDIIGLPVVCSRLFRFSAFYVNKRSNIKTVKDL
                     AGKKVGSPEWAHSAAVYMRGWMHNDMGVKLTDVHWVQAGANAPGRKEKVELNLPKGVK
                     LTRVADKSLSELLASGEIDCAIIARPPACFLEGHPDVERLFPNYHEMEEEYYERTRVW
                     PIMHIIAMRKQILDENPWAARNLYNAFLESKRRSIERLLDPAVSRYPLAWLPTYARTM
                     RDMFDGDPFPYGIEENRPTWEQMALYTWQQGIAHRHFKPKEIFPRGMMTKVVI"
     gene            18114..18857
                     /locus_tag="AC16CX_00975"
     CDS             18114..18857
                     /locus_tag="AC16CX_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3011116.1"
                     /translation="MSAPASDGRVPEALIADLAAASRILAAQGVVDGFGHVSMRHPST
                     PDRYLMARSIAPALVTPDDLIEYDLDSNPCNANGRGSFLERFIHGEIYKVRPDIMSIV
                     HSHSPSVIPFGLVGVPMRAMFHNAAFIAAGAPVFDIREKFGATDMLVSNGAKGVALAE
                     VMGKRDVVLMRAHGSVACGPTLQAAVFRAVYTEVNARIQHWTNALGGGAPMAALDEEE
                     GRLADAPNQTAGKRAWDLWRTQVRSQAGW"
     gene            18979..>19349
                     /locus_tag="AC16CX_00980"
     CDS             18979..>19349
                     /locus_tag="AC16CX_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3011117.1"
                     /translation="MSDAALKDRVDAKSVTERKQRLVDCIRMLERADIIDYNGHCSIR
                     LDADRLLINIGSCQRSRLTVEDICTINFEGNVIEGKGKPPLEFHLHVGIYRARADVKA
                     VVHAHPKWSTFLTMVGESYKPV"
BASE COUNT         3664 a         6257 c         6110 g         3318 t
ORIGIN      
        1 tagtcgcgcc ggcgggcacg ccgcccgcga tcatccagcg cctgagcgcc gaggtcatca
       61 aggcgatgaa gtcccccgcg atccgggaga ggatcgaggg acaggccttc attccggtct
      121 tcgacacacc ggaagcattc gccgccagcc tgaaaaagga acggcagatg tgggcagatg
      181 tcatccgccg caacaacatc acggcggagt agctggcacg gctccgcctg aaggatgaag
      241 gcggaaggat gatggctgac ctacgtcctg ctcccgcact gcggggggtc atccttcatc
      301 cctcatccga gggtgtcttg cctcttgcgc gtcgggcctt gactgctgag tcaatgcaac
      361 cggaagcgcc tcagtgaacc aacctttgcg ttgtcttcgc gacctttcaa aggcggtgaa
      421 tggtgagtgt actgtggaga ttcgtagctt taatttgtaa ttctttgcgt ccttggcgtg
      481 ctttgcggtg aggctgttat tttcctttgc gctctccgcg cccttggcgg tgaagctttt
      541 ttcctgctcg atgcgcgaac gcgctattcc cgcgcgagaa tgaactccgc gatatccagc
      601 cgcgttgcga tccagatgtc acgcttgctg gccgcggttt ggacgatttt ctcgtaataa
      661 tacgcaccgt gcggacgccc gaagatatga gcgtgtgagg tcacgtcgat gatgaccggg
      721 ccttcgcgcc ggtttgcgat tcttttcagc tgctcttcga acgtctcgag catcatgcgc
      781 ggcggattgc cgtacttcat cgacggcata tcgttgacgt cggtcgagag cggaatggcc
      841 acgatcttgc gcttgccgaa cgtctgcacg tagggcaagt cgtcgtcgaa cacgtcgccg
      901 taccacgtgt agccggcctc cgcaagcagc cgcgcggtgt tggcgctcgg ggtgccgcgc
      961 gggctgagcc agcccttgac gcgcttgccc gaaacttttt cgagcagagc cgtgcagcgc
     1021 tgaatgttct tgcgctcttc gccctccgtc atcagcgccg ggatcacgtc catcgcatac
     1081 gagtgcgaca ggatctcgtg tcccgcatct ccaacggcct tgaccgcttc cggcgtgcgc
     1141 tcggcgagta tcgcgctcac catcacgctc gccctggcgc cgtggcgcgc gaacgcatcg
     1201 agcaggcggt aaatgccgcg cttcgcgccg taggccgccc acgatatcgc catgttgtcg
     1261 agcacaccgg gcggcagcgg gttgcccatc gggctgatgc ccggcgcctt cccgtccgac
     1321 cacgcttcca ggcaaacgtt gaaaacgacg gcgatgcgct tgtcgccggg ccacagccaa
     1381 cccgcttgcg tcatttattt cttccccgcg tttccgaatc agctctctac tcgatcctga
     1441 gcccgatcgc cttcaccacc ttggcgtact tctccgtctc gctcttgaga tacgccgccg
     1501 cccgctccgg cgtggacggg gccggctcga cgcccatacc ggcgtagcgc tcgcgcacgt
     1561 cggcggcttc gagcaccttc accgcttcgc ggttcagccg cgccacaaca tccttaggcg
     1621 tcgcggcggg aacgagcaac atgccccaca gtatgtgctc gtaaccgggg acgccggcct
     1681 ccgcgacggt gggcacctcg ggcagaaccg ccgcgcgctt cgcgcccgtc acgcccagcg
     1741 ccctgaccct cccggccttg acgagcggca atgccaccgg cacgcccgca aagtacatcg
     1801 cgacctgccc ggtaatcaca tcgttcaacg ccagcggccc gcccttgtac gacacgtgca
     1861 ccatcttgag ccccgtcatg tagctgaaca gctcccccgc gaggtggtcg gaattgccgt
     1921 ggccggtgga ggcaaaattg agctcgccgg gacgcgcccg agtgaatgcg acgagatcct
     1981 tcaccgtctt cggcggcagc gaggggtgcg tcaccaggat gtgcggggtc gcgctcacct
     2041 gcgtgaccgg cgcgaaatcg cgcagcgcgt tgaagttgag cttgacgtag gcggttgcgc
     2101 cggtcgcgat cccggcgttc gcgaggagca cggtatagcc gtccggcggc gccttcgaga
     2161 ccagctccgc cgcgatattg gcgcccgcgc cggggcggtt gtccaccacc accggctggc
     2221 cgagcccgtc gcccatcttc gaagccagca cgcgtccgat gagatcagta gtgctgcccg
     2281 cggagaatcc gactatcacg cgcaccggtt ttgccgggta gccctgtgcc cagcctggcg
     2341 caacgcacag aacgcaggcc ccaaacatga tcattcgtac ggcgctcttc accattgctc
     2401 accctccctg tccttggatc gcgtgaacaa cagcaagact agcacaccgg cgcggagccc
     2461 tgctccgccg tgactcgctg ctcgcggctc agaaaagccg tgaaatcggg aaatcatgag
     2521 atcgggaaat ggaggtacaa cggactcgct ctcacgccct gacgctctcc cgctctcacg
     2581 ccctcccgcc ctcacgaaaa tgggctcgcc catttgtcgt ggccggggtt tggcgcgata
     2641 cttcccgtac ccctgacggg aggagcgcat gaagcaagcc ctgttgcaat cgctgctcgt
     2701 gctcgccgcc gcggccgttt ccgccggagc cggcgcgcag gcaacttcca aagaacccgg
     2761 ctatccgacc aagccgattc gagtcgtcgt gccgttcacg ccgggcagcg cgaccgatat
     2821 catcgcgcgg atcatcgggc cgaagctggt ggaacgctgg ggccggccgg tggtgatgga
     2881 caaccgtccg agcgccggtg gcgtcgtcgc cttcagcatc gtggcggaag cgacgcccga
     2941 cggccacacg ctcctggtga ccgggtccaa cttcgcgggg agcgcggcgc tctattcgaa
     3001 gctcccctat gatcccgtcg gggacttttc cggcatcagc cagttcgcga gcaccccgct
     3061 cgtcctggtg gtcgcgcccg ctctcggggc gaagtcggtc agggagctgg tcgcgctcgc
     3121 gcgcgagaaa caggggcagc tcaactacgg ctcgaccggg ctcggcagcg gaccccatta
     3181 tggcgccgag ctgttcaagc tggcatccgg catcaacgcc gtgcatgtgc cgtaccgcgg
     3241 ctcacccgaa tcgctcaccg acctcatggc cggccgcgtg catttcgtcc tctcgcccgt
     3301 cctggcggtc tccccattga tcaagagcgg caggttgctg gcgctggggg tgacaaccac
     3361 gtaccgggcg caggcgctgc cggacgtgcc gacaatcgcg gaggcggcat tgcccggctt
     3421 cgagtatcag gggtggtacg ggatgctggc gccgtccagg acaccgcgcg cgatcatcaa
     3481 ccggctcgcg aaggaagtcg cgcacatcct cgagctcccc gagatcaagg agcgcatcgc
     3541 gagccagggc gccatagcca gatcgagcag ccccgaagcg ttcgacaagc tcgtccacaa
     3601 cgaaatcgcg acgcggcgca aggtgtggaa agccgccggg gtcaaggtgg aatgaaaaat
     3661 gggcctcgcc gcccattttt ccgtgagtcg cggttcgtga ctcgcgggta ggccgggttc
     3721 tcgtcacccg taatcgtcac cgtcattcgc accatcttca ggaagcaacg aatggatttt
     3781 tcactcaatg aagtgcagcg cggctggcag caaaaggcgc gcgagttcgc gcgggaagag
     3841 atacgcccgc tgtcgctcga gcgcgaccag gtcgaaggcg gcttcgcgcc atgggactgg
     3901 gacatcatcc gcaagggctc gcagctcggc ttccgcaccc tggccgttcc ggaggaatgg
     3961 ggcgggccag gggcggactt cgtcacgcag ggcctggtga tggcggagct ggcgagcggc
     4021 gacagcgcca tgtcgaaagc gttcagccag aactggaagt ggagccatca gatcgccgag
     4081 ttctgcacgc gcgagcagaa agagcgcttc ctcaagccct tcctcgccga cgacacgtac
     4141 ctgatgggat cggcgagcac ggagccgaac gccgggtccg acaaccgcta tccgccggac
     4201 gacgacgcgc gcgccggcta ccggctgcgg gcggtgcgcc agggcgaggt atggatactc
     4261 aacggggaga agacattcat cgccaacggc agcgtcggca aactcttctt cgtcaataca
     4321 cgcaccgatc cgaacgcgcc catacgcgac ggcacgacga cgttcctggt gccgatcgac
     4381 acgccgggcg tgcgcatcgg caagattttc aacaagaacg gctggcgctt ctatcagaat
     4441 gccgagctcg tgttcgagga cgcgcgcgtg ccgcacgcca acgtggtcgg cgaggtgaac
     4501 ggcggcatga aggtgcgcag cggcgccgcg gccgagttcg gcgacctgga gctggccgcg
     4561 aacgcgctcg gcgtgtgcga cgcggcgtgc gaatccgcaa tgcgccgtgc gaagggcgag
     4621 cggcgcggcg gcaagctcct ctacgaccac cagctcgtgc agctgaagat caacgagatg
     4681 tttgccctga ccgagtcgct gcgctcgttc gtgctgcgca ccgcctggga gatggaccac
     4741 cgcgtgaaat cagccaaccc catcctcgcg atgaaccact cgacgagagt cattcagcgc
     4801 gtgacgcgcc tcaacctgga gctccacgcc tcaagcgagg gcgcggtcga cgccatggcg
     4861 cacaagctcg tgcgcgacgc catcatctgg tcgcacctcg caggcgacaa cacgcagcaa
     4921 ctcaaggccg ttcgccgatt gaccaagtga ttggtgactt gtgactggtc actggatcct
     4981 tgtgccttgt gcttttcgtc cgagcttcac accctcctcc ctcatcccta acagctaacg
     5041 gcgtagatcg tgggtcaata gcgtaaagga tgaaggcaga aggatggagg aaaaccttta
     5101 accaccaggc tttgtttctg ccttctgcct taaagtttgc cacccgtcac tcatcacctc
     5161 gcgggcccaa cccgcgtgaa cccacggcgt ttattccggc ctgatgccac cttcccgaat
     5221 gagcgcctgg tagcgcagat actcttcctg catgagccgc atgaagccat ccggcgtgct
     5281 ctcgggcgtc gactccgccc cctgccgcac gaacagctcg cgcagcgcgc tcttccgcag
     5341 cccgcgcacg atgtcgccgt gcagctgctc gacgatgcgc cgcggcgtgc cccggggcgc
     5401 gacgatgccc gaccagcggt cgaactgcgc ctcgggcagc ccgagctcgg cgatcgccgg
     5461 cacggccggc agcgcggggc tgcgttgcgg cgtcatcacg gcgagcacgc gcaaccgtcc
     5521 ctcgcgcacc ggccccagca ccgtcggcag cgtgagaacc gcgtaatgca cccgtccgag
     5581 cagcatctcg acgaaagagt ccgacacatt cctgaacggc acgtgcatcg catcgatacc
     5641 gagcgcgcgc gtgaacactt cgccggcgag atgcgacgcc gacccgatgc cgagggaggc
     5701 gtagttgagt tcgccgaagc gcgggcgcgc gtacgcggcg aattcgcgcg ccgtgcgcac
     5761 cgggagcgcg ggcgttaccg ccagcacgtt ggtgatcgac gcgacgtgcg cgacgccgac
     5821 gatgtcggcc agggcccggt acggaggctg ctcacgcagg agctcggtga tgaggcgcgt
     5881 gacgccgatc atggcgagcg tgtatccgtc ggcattcgct ttcgacacca cggtgttgcc
     5941 gatcagcccg cccgcgcccg agcggttatc gacgacgact ttctggcggt agaaggcttc
     6001 cagctcgtcg ccgagcgagc gcgcgaagaa gtcgtctgcc gcgcccggag aggacgcgac
     6061 gatgatgcgg atgggcttga cgggatagac ggcgagcacc tccgattgct tcgcgatgca
     6121 cggagccgcg cccaggaaca cggccgacgc gcataatgcg acccgcagtg cgccggtcgt
     6181 caggaatcgc cggaaaccgg ctgcaaccat gaacgggtca gaccccatcg agacgctcag
     6241 tttccatacg cgagagcgcg caggccatag taggcgatga aaacgaggac aaaggcaagg
     6301 gtatagagac cggtcatgaa ataagcctcg gtccaaaggg acttattgcc cagcaccaag
     6361 aaattccggc cccgggtcgg gccgtgaatg accaacacca acaagatcgc catgatcagc
     6421 aaatgaccga tggcgtcgat caggccgaac ttgtagatgg ccagcgcgaa tacgaaggcg
     6481 aaacccagag ccgtcgcgcg cgccagcagg gagacgccgc tcaataccat gaacgccaga
     6541 tcgaattcga cgaaaccggc gagcaccata taagtgcttg gctcgaggcc catgagaaga
     6601 tccggctcgc gcgccagcag cggataggtc cagccgggat agccccattt ttcgatcgcg
     6661 gcccacagca aggtcagcgc cgttcccgcg tagaggacga tgtagcggga catcagccag
     6721 ccggggccgg gcaggcgcgc cgccaggaag tagtaggaca cgccgagcag gatgaggtaa
     6781 tcgagcaggt ggaaaatgcc gtagcggcct gcggcggcga tgaacagacc cgccagtccg
     6841 acgccgatca ggggcacggt gcggcggtcg agagcgcaca gggccatccc cgcctgcatc
     6901 cacggcaccc agcgttgtcc ggtcgtcagc tcgggggtga gaaagaatcc ctggacgacg
     6961 aacccgtagg cgctgaccgc cgcgaggaag acgaacgcgg caccgcgcat gatcccgaac
     7021 gcgacaggct cggtgacggt gtagcggcgc agccgatctt ccaggatgcg cttgcgcagg
     7081 acataccggt cgagccagaa gaagacgtag atcagaagca ccgaggccag gaagaagtgg
     7141 acaaactggc ccgtcaggac gtcgccgatg gggagcggcg gctggttcag gtcatattcg
     7201 gaaaaccatt tgacatgcgc aagggcggga gggccgacac tcaggccggc gagcagcacc
     7261 atgccgcgtc gccacgcaag cagggtggat gcccgcgcgg gcgggtcgcg ggcgagattt
     7321 tgcgctttca ctttgggaat cggcgtcgaa accggcgatc aattgccgga ccggaactgt
     7381 tctctcgccg ttccgattgc tgaaaaaaag ccccgcacag gtggggcttt ctgattcgcg
     7441 ccggcggtca caccggaacg ggttcttcct gctcgacgcg ttttccgggc gcggccggct
     7501 tggccggggt ctgcgtcttg ctctgctgct tcttcagctc ctccgctttc tccagctcct
     7561 tgcgcagctg ttcaagccgt tcatagtcat agcacatgac gagctccttt ccgtatcaag
     7621 cgacccgtgc atcttccttg gcacgtcttg gagttgtgga ttcatgctac gcccggagaa
     7681 caggaaaggc aaacgcgcga ccgccgggca aggcgggcct tcgaacccgt gaatccatgg
     7741 gtcgatgacc caagggatga gtgatgaagg atgagggatg aggggagaac ttcaggattg
     7801 aggattgagc ctcgaatcac aactcacgaa tcacgaccta tggtcttggg gtggaagaat
     7861 gaccaaaggc gcttcctgtg tgccaacctt ggcgcgctta ggtctccttt ctttacgcgg
     7921 gacgaccccg catggcgtga tacccgtatc gaaacctcta ccttagctag gggtgtgttt
     7981 ccgaagatta ttttcctttg cagattcgcg tccctagcgg taaagcttta acccggccgc
     8041 gctgcggtaa actcataatc cgtaagccag ggaggcgaac atgaagctcg tggagcgcgt
     8101 gaagaatatc ctgattcagc cgaagagcga atgggcggtg atcgagcagg agcagacgga
     8161 taccaagacg ctctacacca gctacattgc catcctggcg ctcattcccg cggcggcgac
     8221 ctttatcagc ctcctcggcg ggcgaatcgg gacgggcctc gtcctgggtg ccatcgtcac
     8281 gcaatatttg ctgagcctcg tcatggtcta cgctgtggcc gtcatcgccg aggtcctcgc
     8341 gcccaacttt gacggaaaca gggattcgag cggggcgctc aagctcacga cctacgcgat
     8401 gactgcctcc tgggtcgccg gcgtgtttgc gatcgcgcct ttcctcggct ggctgctttc
     8461 gtttctcggc agcctgtatt cgatctacct cttcttcctg ggcgcgccag tgctgatgaa
     8521 agtgcccgaa accaaggccg tggcctacac cctcctggta atggtcgccg ccattgtcat
     8581 tggcgccgta atcgggttca tcggcaccgg catcatggga ttcggctcaa ccggatcgat
     8641 gatgaggccc aaagacttct gattcgcgga cccaggaaca gcgtggatcg cgggtcgact
     8701 cagctgagga ctcgtgactc gcggctcgtg actgggtcga cgcaccacac ctcatcgata
     8761 cttgtcatca cgtgaccttg tcactgtgtc actccaggac acatcgatga atccaggggc
     8821 gcggctcttg tgttttctcc tgctggcgtc cgcgtggggt ggtgcgtcgg cgcagagcta
     8881 tccgatcaag ccggtccgcc tcatcgtggc cagctcggcg ggcagcaatc ccgatgtcct
     8941 ggcccgcatc gttgcggtgg gcctcacgca ggccctcggt cagcaggtca tcgtcgagaa
     9001 tcgcgccggg gccggcggta acattggcgc ggaagtggcg gcaagggcgc cggcggatgg
     9061 gtacaccgta ttcctcgtcc ataccaatca tcccatcaat accgcgttat accccaagct
     9121 cgcttatgac cttctcaccg actttgcctc gatcacgcaa ctggcaatgt ccgcgttcgt
     9181 gctggtcgtt catccctcct tgcccgccaa gtccgtgcgg gacctggtga cgctcgcgaa
     9241 ggcgcgtcct ggcgcaatca ccttttcttc ggctggcacc ggcagcggca cgcacctgtc
     9301 agccgaatat ttcaacggca tggtgaaggt gaagatgctg cacgttccct ataatggcgg
     9361 aggtcccgcc ttgaccgcga tcgcttccgg agagacgtcg gtgtatttcg cgcccgtctc
     9421 gaccgggctg ccgcacattc tcagcaacaa gcttcggccg ctcggcgtga cggcagccag
     9481 gcggctacgc gagctgcccg acgtgcccac gatcgccgcg accgtgcccg gatacgagtt
     9541 cacgagctgg gcgggcctga tggtgcccgt caagacgccg aagcatgtca tcgacaccat
     9601 gcacaaggct gcaatcgccg cgctgaagaa ccccgagacc agcaagcgcc tcgacaccgc
     9661 cggtttcgtc gcgctcggga accggcctga ggacctttcg gcccacatca agtccgagat
     9721 cgagaaactg gcaaagctga ttcgccaaat cgggctacgc ccggagtaag gagcagcgcg
     9781 atgggccgta aagtgaatac aggagatcca acatgaacca caaattcccg agcgccgccg
     9841 tcgtgaccgc agccattctc cttgccggct gcgcggggca gcgcgccgcc gacactgcgg
     9901 aaggccccgg cgcgcggccg cgtggcgtgc cggtgttcga ggtagacccc tcgtggccga
     9961 aggtgccgga aaaatacaag ctgggagatg catccagcat cggcatcgat gcgcaggaca
    10021 acgtatgggt cctgcaccga ccgcgcacac tgaagcccga ccaggcgcgg aatgccgcgc
    10081 cggcaatcct ggtattcgac ccggcgggca acttcatcaa gacctggggc ggggcgggaa
    10141 gcggcttcga gtggcccgaa cgggagcacg gcatccacat cgatcacaag ggctatgtct
    10201 ggctgggcgg aaacaattgt cccgcgagga acctgcccgg gctgaagccg gccgcggacg
    10261 accaactcct gaagttcacg caggacggca aattcgtgat gcagatcggg cgagccaacc
    10321 gcagcaaggg caacgccgac acgctgaacc tgagccagcc ggcggacgcg tgggtccacc
    10381 ggaagaccaa cgaattgttc gtcgccgatg gctacggcaa ccaccgcgtg gcggtgttcg
    10441 acgcggacac cggcaggttc aagcgcatgt ggggcgcgtt cggcaacaag ccggtggatg
    10501 actaccggtg cccgccgccg agtctcaagg cggttccgga cggtccgggc cccgaccaat
    10561 tcagcatcgt gcacgccatc cgcgtcgcga acgacggcat ggtgtacgtg gccgaccgcg
    10621 aaaaccggcg ggtccagatg ttcacgagcg acggcaagtt catcaagcag ttcgtcaggc
    10681 acaacgcgcc gttcgcgcgg aacctggcgc tctcccccga tccggagcag cggttcctgt
    10741 acgtcggcgg agggattgat atcgtcgtgc tggagcgcaa gacgctcgaa ttcgtggacg
    10801 tgatcggcgg gccgggtttc ctgggcggcg gccaccagat cgcgaccgac tcgaagggca
    10861 acatctacgt cgccgccacc actcgtggct tacagaagct cgccttcaaa ggcgtgaggt
    10921 gaggaaacca gcgaatgcca tccgacccga aatccgtcac tcccgccgag tcgccgcgcg
    10981 tgacgcggct gcgaacgctg ctgggggatt accccaccac ccgggccctg aggcggggcg
    11041 agttgagctc gtcccgtgtc gccttcgatt ttgccgacgt cccgcaggta gccaacgcgt
    11101 tcaagcgcac cgtgcggaat ctcgagttcg atgtctccga gctcgcgatc gtgaccttcc
    11161 tgatggcgaa ggcgcacggc aagccgtacg tgctgctgcc ggcggtggtc ctgggccgct
    11221 tccagcaccc gttcctcgtg tacaactcgg cgcgcggcga gctgcgcccc caagacctca
    11281 acgggcgccg cgtcggcatc cgctcctact cggtgacgac gagcatgtgg atccgtggaa
    11341 tactcgccaa cgaccatggc gtgaacatcg attccatccg ctgggtgacg ttcgaggaac
    11401 cgcacgtggc ggaattcagg gacccgcctt cggtcgagcg cgcccccgcg ggccgcgagc
    11461 tgctctcgat gctgctggat ggcgaggtgg acgccgcggt gctcagggac tcgaacctga
    11521 cggacccgcg cctgaagcga atcattccgg acccggacca ggccgcgcgc gactggcacg
    11581 tgcgcaaccg cgcgatccag atcaaccaca tggtggtggt caaggaatcg ctttcgaagt
    11641 ccgacccgga tgcggtgcgc gaagtgtacc ggctgctccg cgaagccaag cgcctcgggc
    11701 ccgctcctag cacgcccgac atgacgccga tcggcctgga ggcaaaccgc cgcaatctcg
    11761 aggtagcgat cgactacgtc caccagcagc ggctcattcc gcggcgctac gcggtggacg
    11821 agttgttcga cgacgtgacc agaaagctag cctgacggcg gtgatgggtg atgaagtggc
    11881 gaataacgac gcagaaaagg ttgtgccgaa ttcctcgggt accgggcgac ccaggatcaa
    11941 ggcgccagcg aacgcggccg attgccacat gcacatctac gatccgcgct tcccgcagct
    12001 cgcgtcgagg tcgcccgtag ccaacgccgc cgtgccggat taccggctgc tgcagaagcg
    12061 catcgggacg acgcgtgtgg tggtcgtgca gccgcgcaac cacgcgacgg agaatcaggt
    12121 cacgctcgac gcggtggccc agctcggccc gggtgcgcgc ggcatcgcgg ttctgcatcc
    12181 cacggtgacg gacgccgagc tgaaaaagct tgacgccggc ggcatccgcg gcatccgttt
    12241 ttcgctcggc gacccggcca ccgccgtcgt cacgatcgac atgctcgagc cgctttcgaa
    12301 gcgcgtcgcc gcactcggct ggcacgtgca gctgcacatg cctggcgaca tgatcgcgga
    12361 gaacgcggac ctgctgcggc gcctgccatc gcagatcgtg attgaccaca tgggacgctt
    12421 cccgccggcg gtcggtctcg ctcaccccgc gtaccaggtc atcctcgggc tgatcgaaaa
    12481 gggccggacc tgggtcaagc tggcgggcgc gtacctgaac acgcggattg gcccgccgac
    12541 ctacgcggat gcgacgaaaa tcgcgcgggc gttcgtgaag gcggcgccgg agcgcctggt
    12601 ttggggcagc gactggccgc acccgaccga gaagaacaaa ccagacgacg cccagctctt
    12661 cgacctgctg gccgaatggg cgatggacag gaaggcgcgc aaccgcatcc tcgtcacgaa
    12721 tccggaggtg ctttacggct tcgcgaaaac cgggtgacga gggaaaggca atgatgaagg
    12781 cggaaggagg aaagaaagca agaacaagct ttctttggcc ttccaccttc cgccttcctc
    12841 cttccgcctt cgtaagtcat cagccgtcag tcgtcactca atctgcgctt cgcttgatcc
    12901 tgatcaacag cggggaaccg gcgtctgaca ccattcggct catccagctg acccggctcg
    12961 tgaccgggaa ggagaaaagc catgcgcacc acgaagcgaa gggctaaacc cggttcggtg
    13021 gggagaggcc gcttttttca catcgtcgtg aggccaaatt ctcagttcgt ccgcttccgc
    13081 gtccaggata tcggagcccg gggcggcgtc gagcgcgtcg ccggccagcg cagcaacggc
    13141 tcctgggaca cggtgaaatg gctggtcgag aaaacccacg cgcacgtccg cggcgggacg
    13201 ctggtcgccg acaccgccga ggcgcgcaag ctgctgcgca gcctgggctc ccggccggtg
    13261 cacctcggcg gcgatcgttt ccgggcggcg ccgcgcgcga acatccccga gagcgaaaag
    13321 cccacggcag gacaactcag agcctggaag cgcaacatcc gcaaggcgca gcaggcgagg
    13381 cgcgggggac gctgacgcgt ctccctaagg atgagggatg aaggatgagg gatgatcccc
    13441 gcagtgcggg agacctatca cccatcactc atcacccatc accggttcta gatcgagcgc
    13501 cttcgcctct cgcgcaccag ttcctcgagg ccgcgaatga cggtctcaag atctgtcagg
    13561 ttcggattca ccttgagcgc ccgccagaaa tattcgatcg ctttttcgta ctcttccagg
    13621 cgaacgtaga tctgcccgta gcccgacagc gcgccgaagt gctgcggatt tctcttcatc
    13681 acctcgtcgc agtcggcaag ggatttgcgc atctcgccgg ccaggtaata tgcggtcgcg
    13741 cgcttgttcc agccttccgc gaagtccggc ttgcgcacta tcaccttgct gaaaatctcg
    13801 atcgcccgcg ggtagcggcc cgcgttcatc tccgcagcgc cggtttccag caaccggtcg
    13861 gtctccgcgt cgcccgagcg gctccacgcg agccagacgg cctgtccggc cacttcacgc
    13921 acgacgggat tttcgtcaga gaggcgtttc accagcagcg gcccgtcggc ggcgaggccg
    13981 ttgcgcccga aatagacgac gcccgcgagg cggaagcggg cgtcggcgtc ttcgagcgcc
    14041 ttgagcgccg ccgcccgtga ttcaagctcg gggaatggcg gctgggcggc ggcggtcagc
    14101 gccaggaagg cggcgaaaag cagcccaagg atccgcggca ttgtcgaatc aaacagaggg
    14161 ggcggagtca ctttcatgga atccggaatt ttacgccggc cctcaataaa cggggttggc
    14221 ccacattaag atacttatcg ccgattctgc gataatttgc gccccactcc gctaaccaag
    14281 gagagacaac aatggccgtc tcgatgcacc cgatttccct gcccgtgttc gttcgccacc
    14341 tgaccggcct cgccggctgc ctgaagaagg cgcaggcgct ctacgccgag aagaagtacg
    14401 acgaggccac cctggtgagc taccggttct atcccgacat gttccacttc gcccggcagg
    14461 tccaggccgc gaccgatcat gcaaagggct gcgccgcgct gctcgcgggg ctggaagcgc
    14521 cgaagtatga ggacaacgag aggagcctcg cggacctgat cgcgcgggtg gagaaaacga
    14581 tcgcctacct gaacacgatc cagccggggc agatcgaagg tgccgaagag aaggccgtca
    14641 cggtgaaaat gcgggaccgc gaactgacct acaaggggct ggacctgctg ttgaagcgct
    14701 ccctgcccaa cttcttcttc cacgtcacga ccgcctacga tatcctccgg cacaacggcg
    14761 tggagatcgg gaagcgggac ttcatgggcc ccaactaagg gattaagcaa gctcttcctg
    14821 gtgtttactc cgtcatcctt acatcttcat tcttcggccg actcatcatc catcacgcat
    14881 taaccgaaag gagcgaccca gatggaacga aagaaagcca gcgatttccc gcaggaagtc
    14941 ctcaaactat tcgacggata cgtgcatggc ggactgacgc gccgccagtt cctcgaccgc
    15001 gccgcgaagt acgcggtggg cggcttcacg gccgccgcga tgctggaggc cctccgcccc
    15061 aatttcgcgt gggggcagca ggtcgcgacg gacgacaagc ggatcaggac cgaatacctg
    15121 acctactcct cgccgcaggg ttcgggaacg atgaagggat atttagcgcg gccggcgaag
    15181 gtcgagggca aattgcccgg cgtgctggtc atacacgaga accgcggtct caacccgcat
    15241 atcgaggacg tcacgcggcg cctggcggtg gtgggttacg tggcctttgc gccggattct
    15301 ctcacgccgg tcggcggcta ccccggcgat gaagacaagg cccgtgatct ctttgccaag
    15361 caggatccca aaaaacggat cgaagacctg gtggcgggcg cgtactttct caagtcgcac
    15421 cccgaatgca acggcaggat cggcgccgtg ggcttctgct tcggcggcgg catctgcaac
    15481 gtgctggcgg tgcggatacc ggacctcgcg gcgtcggtgc cgttttacgg catccaggtc
    15541 agcgccgagg atgccgcgaa gatcaaggcg ccgctccaga tccactacgc cgagaacgac
    15601 gagcgcatca acaagggctg gcccgcctac gaggccgcgc tcaaggcgaa cggcgtgaga
    15661 taccagatgt acatgtaccc gggcacccag cacgccttcc acaacgatac gacgccccgc
    15721 tacgacgaag gcgcggccaa gctcgcctgg tcgcgaacgc tcgctttttt caaggagcac
    15781 cttaaagggt gaggtttttc agtccgcgcg cacgcccgac tccttgatca ccttagccca
    15841 tttcgcgact tcgctgcgga tgtatgcccc aagctgctct ggcgtcgaag catagggctc
    15901 gaggtcggtg ggggcgaacc gttcctttac cgcaggatgc gcgagcgcgc tcacggtcgc
    15961 cgcatgcagc cgcgcgatga tttctttcga cgtgcccgcc gggaccacga ggccgtacca
    16021 agtggtcaca tcgtagccgg gcacgccggc ctcggcgagc gtcgggatgt cgggcgccat
    16081 ggaggagcgc ttcggggtcg tgaccgcgag cacgcgcagc ttgccggccc tggcatgcgg
    16141 cagagtcgcg ctggtggcgg gaaaaccgat cgacgcctcg ccgctgatca ccgcggtgac
    16201 ggcctgcggc gtgctcttgt aattgacctc cgtcgtcttg atgcccgcca tgccgtcgag
    16261 catcttggct gccagccgta tcgccgcgcc cgccgtcgcg acattgattt ccccaggccg
    16321 cttcttcgca aacacgatca gctccttgac tgatttcgcc ggcaccgagg ggtgcaccgc
    16381 gaggcagaac gagccggagg caagcgagct gaccggcgca aagtcccgga cgatgtcgaa
    16441 tccgagtttc gagtaaagcg tcatgctgat ggagtgggaa atgttggcca tcagcagcgt
    16501 gtagccgtcg ggtggcgcct tggcggcgac ttccgccccg aggttgccgg ccgcgcccgc
    16561 gcggttgtcg atcaccacct gctggccgag cgccgcggag aattgtggct ggacgaagcg
    16621 cgcgaccgtg tcgctgccgc tgcccgccgc aaacggcacg atgaaccgta tggtgcgggt
    16681 cgggtattga gcatggaccg gcgcaattcc tgccagcgca atcgtcagaa caatcaaacc
    16741 tctcaacgtc attgttgtca tgtttcctcc tcgaaaagac cgccgtccac acccgtacca
    16801 cgcctgcctc aacttcggat tatcactctg aagtgcgact caatggcgac gccccggtca
    16861 aaacgtttac aaaatcaatt atttgttata gatttcctcc cccgccgctc ctgaagcagg
    16921 aaggcggaag gcagaaggat gaagggggaa cccgtcactt cgtcactgcg tcactcagcg
    16981 gagatgaagt cggagggata aaggggggaa catgtcgcaa ctgaaacttt ccatcgcgac
    17041 gaccgattac gaccatttcc gcgatttccg gctgggtgtc gtgacggccg aaggaatcga
    17101 gctcaactgg ctcaatctcg gccaccacga gtgcttcgcg cgcttcaccg ccaaccggga
    17161 gttcgacctg tcggagcttt ccttcgcgaa gttcgccgcc caggtgacgc gcgaggacag
    17221 cgacatcatc gggcttcccg tcgtctgctc acggctgttc cgcttcagcg ccttctatgt
    17281 gaacaagcgg agcaacatca agaccgtgaa ggacctggcc ggcaagaagg ttggctcgcc
    17341 ggaatgggcg cattcggcgg ccgtgtacat gcgcggctgg atgcacaacg acatgggcgt
    17401 gaaattgacg gacgtgcact gggtccaggc gggcgcgaat gcgcccggcc gcaaggaaaa
    17461 ggtcgagttg aatctcccca agggggtgaa actcacgcgc gtcgccgaca agtccctgag
    17521 cgagctgctg gcgagcggcg agatcgactg cgccatcatc gcgcgcccgc cggcctgttt
    17581 cctggaaggc catcccgacg tggagcgcct gtttccgaat taccacgaga tggaagagga
    17641 atattacgag cggacccggg tctggccgat catgcacatc atcgcgatga ggaagcagat
    17701 cctggacgag aacccgtggg ccgcgcgcaa tctctacaac gcgttcctgg aatcgaagcg
    17761 ccgcagcatc gagcgcctgc tcgatcccgc cgtgtcgcgc tatccgctgg cgtggctccc
    17821 cacttacgcg cgcacgatgc gcgacatgtt cgacggcgat ccgtttccct atgggatcga
    17881 ggaaaaccgc ccgacctggg aacagatggc gctctacacc tggcagcagg gcatcgcgca
    17941 ccgccacttc aagcccaagg agatcttccc ccggggcatg atgaccaagg tcgtcatcta
    18001 actgcaagga tgaaggcgga aggcggaagg atgaccggga tttcactccc gcgctgcggg
    18061 ggtcatccct catccttcat ccttcatcct tcgggtgata cgccacccgc tttatgagcg
    18121 cgcccgcatc ggatggccgg gttcccgagg ccctgatagc ggacctcgcg gccgccagcc
    18181 gcatcctcgc cgcccaaggc gtcgtggacg gcttcggcca cgtctcgatg cgccatccgt
    18241 cgacgcccga ccgctacctg atggcgcgct cgatcgcgcc ggcactggtg acgcccgatg
    18301 acctcatcga gtacgacctc gacagcaatc cctgcaacgc caacggccgc ggcagctttc
    18361 tcgagcgctt catccacggt gaaatctaca aggtccggcc ggatatcatg tccatcgtgc
    18421 acagccattc gccgtcggtg atcccgttcg gtctcgtcgg cgtgccgatg cgggcgatgt
    18481 tccacaatgc cgccttcatc gcggccggcg cgccggtgtt cgacatccgc gaaaagttcg
    18541 gcgccaccga catgctggtc agcaacggag caaagggcgt ggcgcttgcc gaggtcatgg
    18601 gaaaaaggga cgtcgtgctg atgcgcgctc acggcagcgt tgcatgcggg ccgacgctgc
    18661 aggctgccgt attccgggcg gtttacaccg aggtcaatgc gcgcatccag cactggacga
    18721 acgcgctcgg cggcggcgcg ccgatggccg cgctcgatga ggaggaaggc cggctcgccg
    18781 acgcgcccaa ccagaccgcc ggcaagcgtg cctgggatct ctggcgcacg caggtgcgaa
    18841 gccaggccgg ctggtaggaa gcctggcttc gcggtgatga gtgatgggtg ataggtgatg
    18901 ggtctcccgc aaagcagcgc ttttgaactc ctatcactta tcacttatta cttatcacgg
    18961 gtttgaaagg agcatgccat gtcagacgcc gcattgaagg accgggtgga cgccaagtca
    19021 gtgaccgagc ggaagcagcg gctcgtcgac tgcatccgca tgctcgagcg cgccgacatc
    19081 atcgactaca acggccattg cagcatccgc ctcgacgccg accgcctcct catcaatatc
    19141 gggtcgtgcc agcgcagccg gcttacggta gaggacatct gcacgatcaa tttcgaagga
    19201 aatgtgatcg agggcaaagg caagccgccg ttggagttcc acttgcacgt cggcatctac
    19261 cgggcgcgag cggacgtgaa ggcggtcgtg cacgcgcatc cgaagtggtc cacgttcctg
    19321 accatggtcg gcgagagcta caagccggt
//
LOCUS       DBNGWZ010000034         6599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_381001, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000034 DBNGWZ010000000
VERSION     DBNGWZ010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_381001"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1177
                     /locus_tag="AC16CX_00985"
     CDS             311..1177
                     /locus_tag="AC16CX_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011118.1"
                     /translation="MAWPPGGAADVIARVVAQKLGEQLGQQLVVDNRPGASGAIGTGL
                     VAKSAPDGYTLLVGGVSELVLNPQIVKVSYDTARDFIAVSSLAFGYYILVIHPSLPAT
                     SVKGLIALARARPGEIKYASGGTGSNLSLVAELFKTTARIDVSHIPYKGGGPAGVAVL
                     SGESQMMFSTIASTLAYVKSKRLVALALTGTKRSPLLPEVPTFAESGLRGMDVGLWFV
                     LLAPAATPREIVARLNAEAVKLGAAPDYRSQLEKVGFEPFTGSPEQCSEFLKTELDRW
                     GKVIKAANIKSD"
     gene            1269..1418
                     /locus_tag="AC16CX_00990"
     CDS             1269..1418
                     /locus_tag="AC16CX_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011119.1"
                     /translation="MKRSIGLLLLAVWLIFTGLIPLLNFSFTGMSTVMAVLAIAAGIL
                     IVVGR"
     gene            1509..1811
                     /locus_tag="AC16CX_00995"
     CDS             1509..1811
                     /locus_tag="AC16CX_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3011120.1"
                     /translation="MLWAISRVAAPNFAAMREKGLQAHLDYLRSQKDILVLSGATLTD
                     DGKDYVGSLLIVNVSSRAEAKAFVDGDPFQKAGMFTSVTITRMRKGQWNPEAAEGA"
     gene            1952..2764
                     /locus_tag="AC16CX_01000"
     CDS             1952..2764
                     /locus_tag="AC16CX_01000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3011121.1"
                     /translation="MADKPSVAFVGSMPENYDRYLGPAHFVPFADDLAGRVASARPRG
                     AVLEIACGTGIVTKRLRERLPSSVRLVATDLSQPMLDYARAKLAAAPVEWQQADAMAL
                     PFEDSSFGAVVCGFGVMFVPDKSVAFREARRVLTTGGSFVFNVWDGFEGNECSRIVFE
                     TIRTFFGGQEPEFLKIPFGFNDHNLIRRLLAAERFDGIEIDPVTIKASSPTARDFATG
                     VIRGTPAGHEINQRGIPFDDVVEALAGALARALGDKPFRADRRALVVTARAA"
     gene            2870..3019
                     /locus_tag="AC16CX_01005"
     CDS             2870..3019
                     /locus_tag="AC16CX_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011122.1"
                     /translation="MLHNPRVRARFLNAGVEVVGSSPEQMLDAVKSEMARMGPVIRAA
                     GIRSE"
     gene            3102..4112
                     /locus_tag="AC16CX_01010"
     CDS             3102..4112
                     /locus_tag="AC16CX_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011123.1"
                     /translation="MIGKTLRWMYLALLAGCATAPSADAPGRFDIGLIGDQHYDARSE
                     AQFPNIMAAMNRASLAFVVHVGDIGAPKYNSCRDETYYRRRDEFNQSRHPLVFTPGDN
                     EWTDCHETGVPDSLERLAKVREVFFPGERSLGQRTIPQTRQSSDPRYVQYRENARWSL
                     GGVLFVTLHVPGSNNNLGRTPEMDTEYAERNAANLEWLRQAFQVAKRDGSLAIMLLIQ
                     ANPRFEATFPARRRGALGVAPPPKRVSGYADFLDVLRREVLAYDRPVALVHGDTHYFR
                     VDKPMILDDKSGAGRGRIVEHFTRVELFGYPEAHWVRATVDPADPNVFNFRPQIVKEN
                     VP"
     gene            4229..4534
                     /locus_tag="AC16CX_01015"
     CDS             4229..4534
                     /locus_tag="AC16CX_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3011124.1"
                     /translation="MPIGEEVILKTENARVRIIELGPNEVASLHSHNEVTDNIFCLSG
                     RMSVRMKDPDEEAFLQPGQRCTVAQGRVHQVVNNEATHTTYLLVQGVGRYDFNIHKS"
     gene            4675..5004
                     /locus_tag="AC16CX_01020"
     CDS             4675..5004
                     /locus_tag="AC16CX_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR3011125.1"
                     /translation="MAFDEQLAAKVRKELTGQLGLVEKKMFGGLAFLVNGNMSVGVYR
                     DELIVRLAPEETEAALKEPGARVFDITGRPMKGWLMVGGAGLEQPSSLAKWVRRGVGY
                     ASSLPKK"
     gene            5141..5887
                     /locus_tag="AC16CX_01025"
     CDS             5141..5887
                     /locus_tag="AC16CX_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3011126.1"
                     /translation="MTTPSIEFRVMTSGAFTAAHLALMPELERLTKAKVVTASTSIGT
                     GETSIPNRLKRGEVVDIVIVAESVLRQFIEDGLVLAEGHAVIARSSIAVAVRAGAPKP
                     DISTVDALRRTLLQAKSIGYSASESGKYFSTELVQRLGIADQVLGKSRLVGGGERVGA
                     VVARGELEIGFQQLSELLPVKGIAHITLLPPELQKVTSVAAGVAASSPNKALARSAIA
                     FLASPEAAQAIANTGLEPTALSRGRTTAMH"
     gene            5958..6350
                     /locus_tag="AC16CX_01030"
     CDS             5958..6350
                     /locus_tag="AC16CX_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011127.1"
                     /translation="MQILQPPGWKKPRGYSNGIVAKGRTVFVAGQVGWNADEKFETSD
                     FTGQARQALKNIVAILAQAGAGPEHICRMTWYVADRHEYNASLKGLGAAYREVIGRNF
                     PVMTAIQVAGFVEEGAKLEIEVTAVIPE"
     gene            6469..6599
                     /locus_tag="AC16CX_01035"
                     /pseudo
     CDS             6469..6599
                     /locus_tag="AC16CX_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135251415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1343 a         1964 c         2093 g         1199 t
ORIGIN      
        1 ttatcctttg aggcaacggc gaaaatgatg caggagatgc tgctgccgac gatggcagtc
       61 tatgccgacc ttggacgcag tctgtatgag gggctgctca agcccaagcc gccacgcagg
      121 gcgcgaaagc acaagatggc tcggtcgagg aaaagggtct cttgatcccg gtcagaccac
      181 cgcaacgcat tgatcgagaa tgatgaccgc acgccgtttg atttgcctga tggcaagtct
      241 attgccgctg gcggttgcaa cgaccgcgat cgcacaacct ggatacccag cccggccagt
      301 ccgcgcagtc gtggcctggc cgcccggcgg ggctgccgat gtcatcgcgc gggtggtggc
      361 ccaaaagctc ggtgagcagc tcggccagca gctcgtcgtc gacaaccgtc ctggcgcgag
      421 tggggccatc ggcaccggcc tggtggcgaa atccgcgcct gacggataca ccctgctcgt
      481 tggcggggtg agcgagctcg tgctcaatcc ccagatcgtg aaggtgtcgt acgacacggc
      541 gcgcgatttc atcgcggtga gctcgctcgc gtttgggtac tacatactgg tcatccatcc
      601 ttcgctgccg gcaacttcgg tcaaagggct gatcgcgctg gccagggcgc gccccggcga
      661 gatcaagtac gcatccggcg gtaccggcag caacctgtct ctcgtcgccg agctgttcaa
      721 gaccacggcg cgcatcgatg tatcccacat tccgtacaaa ggcggcggcc cagcgggggt
      781 ggcggtgctg tccggcgaat cgcagatgat gttctccacc atcgcttcga cgctcgcata
      841 cgtaaaatcg aagcggctgg tggcgctggc cctgacgggc acgaagcgct caccgctgtt
      901 gccggaagtt ccgacgttcg cggaatccgg tttgcgcgga atggatgtgg gattgtggtt
      961 cgtgctgttg gcgcctgcgg ccacaccccg cgagatcgtt gcgcgtctca acgctgaagc
     1021 cgtcaagctt ggcgccgcgc ccgactatcg ctcgcagctc gagaaggtgg gattcgagcc
     1081 gttcacgggc agtcccgagc aatgttccga attcttgaaa accgagcttg accgatgggg
     1141 caaggtgatc aaggcagcta atatcaagtc cgattaaaag aagagtctta atccgggtca
     1201 ggtcggacca ccgcaagaaa ataaatgggg tctgagctgt cctcaatttc ccccaaagga
     1261 gtctgtcaat gaaaagaagt atcgggttgt tgttgttggc cgtatggctc atcttcaccg
     1321 gactcattcc gctgctcaat ttcagcttca cgggaatgag caccgtgatg gctgtcctcg
     1381 ccatcgcagc cggtattttg atcgtcgtcg gccgatagcc tgtgtagcgg ggtcaggtct
     1441 tgtcttttgc ctggaccggc tatcgatagc aaaaagcagg acttgacccg aaagcccgaa
     1501 aggtaagtat gctctgggcc atctcccgcg tagctgcgcc gaacttcgcc gcgatgcgcg
     1561 agaagggctt gcaggcccac ctcgactatc tacgtagtca gaaggacata ttggtactct
     1621 cgggcgcgac gttgaccgac gacggcaagg attacgttgg cagcttgttg atcgtcaacg
     1681 tgagcagccg cgctgaagca aaggccttcg tggacggcga cccgtttcag aaggcgggca
     1741 tgttcacgag cgtgacgatc acgcgcatgc gcaaaggaca gtggaatccc gaggcggcgg
     1801 aaggcgccta atctgaaggc ggaagtcgga agtcggaagt cggaagtcgg aaggcggaag
     1861 gcggaaggcg gatcgcgcgc cgagccgtca gctgtcaccc gtcagcaaca ataaatagcg
     1921 tcagacccca ttaaactctt accatacggc catggccgac aagcctagcg ttgctttcgt
     1981 agggtcgatg cccgagaact acgatcgtta tctcggcccg gcccacttcg tgccctttgc
     2041 ggatgatctg gctggccgcg tggcatcggc ccgcccgcgg ggcgcggtgc tggagattgc
     2101 gtgtggcacg ggcattgtca cgaaacggtt gcgcgagcgc ctgccgtcat cggtccgctt
     2161 ggtcgccacc gatctgagcc agccgatgct ggactacgct cgcgccaagc tggcagcggc
     2221 tcccgttgaa tggcagcaag ccgatgcgat ggcgttgccg ttcgaggatt cctcgttcgg
     2281 cgccgtcgta tgcgggttcg gcgtaatgtt tgtcccggat aagtccgtcg cattccgcga
     2341 agcgcggcgc gtgctcacca ctggcggatc gtttgttttc aatgtctggg acgggttcga
     2401 ggggaatgag tgctcgcgaa tcgtgttcga gaccatccgc acgtttttcg ggggtcagga
     2461 gccggaattc ttgaagatcc cgttcgggtt caacgatcac aatctcatcc ggcggctgct
     2521 cgcagccgag cgattcgatg gaatcgagat tgaccccgta acgatcaagg cgagcagccc
     2581 cacggcgcgg gactttgcca ctggcgtgat ccggggcacc ccagccggtc atgagataaa
     2641 tcaacgcgga attccattcg atgacgttgt cgaggcgctc gccggcgcgc tcgctcgtgc
     2701 gctgggcgac aagccattcc gcgcggaccg gcgggcgctg gtggtgacgg cacgcgcggc
     2761 ctagcgtaac tggcttgggg tcggaccacc gcaacatgga gcaaaggggt cagagccgaa
     2821 atatcttctt gccttggttc agaaattatt tcggctctga ccctttttca tgctgcacaa
     2881 tccgcgggtc cgggcgcgat ttttgaacgc cggcgtggaa gtcgtgggca gctcgccgga
     2941 gcagatgctc gacgcggtga aatcggagat ggccaggatg ggcccggtca tcagggcggc
     3001 cggcatccga agcgaatgag gggagccgcg cgaaaatcac ccctgcctca ttcatcacgg
     3061 atacgctatc gtaatccgtt ttggcctgga gaggaggacg tatgatcggc aagacattga
     3121 gatggatgta cctggcgcta ctggcgggct gcgccaccgc gccgtctgca gacgcgccgg
     3181 gccggttcga catcgggctg atcggcgacc agcactacga cgccaggtcg gaagcccagt
     3241 tcccgaacat catggccgcg atgaaccgcg cgagcctcgc cttcgtcgtc cacgttggcg
     3301 acatcggcgc gcccaagtac aactcgtgcc gggacgagac ctactaccgg cggcgtgacg
     3361 aattcaacca gtcgcgccac ccgctcgtgt tcacgcccgg cgacaacgaa tggaccgact
     3421 gccacgaaac cggcgtgccc gattcgctgg agcggctagc caaggtgcgc gaggtgttct
     3481 tcccgggcga gcgcagcctc gggcagcgca ccattccgca gacgcggcag agctcggacc
     3541 cgcgctacgt gcagtaccgc gagaacgccc gctggagcct cggcggcgtc ctgtttgtca
     3601 cgctgcacgt gccgggcagc aataacaacc tcggccgcac cccggagatg gacaccgagt
     3661 acgccgagcg caatgcggcc aacctagaat ggctgcggca ggcgttccag gtcgcgaagc
     3721 gggacggcag cctcgccatc atgctcctga tccaggccaa cccgcgcttc gaggcgacct
     3781 tccccgcgcg ccggcgcggc gctctcggcg tcgcgccgcc gccgaaaagg gtgagcggtt
     3841 acgccgattt cctcgatgtg ctgcggcgcg aggtgctcgc ctacgacagg ccggtcgcgc
     3901 tggtgcacgg cgacacccac tatttccgcg tggacaagcc gatgatcctc gacgacaaga
     3961 gcggcgccgg ccgcggccgc atcgtcgagc acttcacacg cgtcgagctg ttcggctatc
     4021 ccgaggcgca ctgggtgcgc gcgaccgtgg accccgcgga cccaaacgtc ttcaacttta
     4081 ggccgcagat cgtgaaggaa aacgtgccgt gattcgcgcg aaaggcggaa tccttagagg
     4141 accgaccacc aaggaaactg attaaaccat ctgtacaatc tggggcagct tggtctgacc
     4201 cttgaacttc catttgaaag gaagcgacat gcccatcggc gaagaggtca tactcaagac
     4261 ggaaaatgcc cgcgtcagaa ttattgaact ggggccgaat gaagtggctt cgctgcactc
     4321 tcacaacgaa gtcaccgaca acattttttg cctttccggc aggatgtccg ttcgcatgaa
     4381 ggacccggat gaggaagcct ttctgcagcc cggacaaaga tgcacggtgg cacagggcag
     4441 ggttcaccag gtcgtgaaca acgaggcgac gcacacgacc tatcttttag tccagggcgt
     4501 gggccgttat gacttcaata ttcataagtc ctgattccgg gtcggaccac cgcaatgctt
     4561 tgatcgtcaa gacaacgttg ctggaaaagt gtttttcgag ggcttagaag ccactttttg
     4621 gggccttagg ggacagaccg cagctaagat ggtcagtacc gaggagatcg gcgcatggca
     4681 ttcgatgagc agctggcagc caaagttcgc aaggagctga ccgggcagct cggccttgtc
     4741 gagaagaaaa tgttcggcgg gcttgcgttc ctggtgaacg gaaacatgag cgttggggtg
     4801 taccgcgacg agctcatcgt caggctggca ccggaggaaa cggaggcggc gctcaaggaa
     4861 ccgggagcgc gcgtattcga catcacgggc cgtcccatga aggggtggct gatggtcggg
     4921 ggcgccggcc tggagcagcc gtcctcgctc gcgaagtggg tccgccgcgg cgttggctat
     4981 gcgtcgtccc ttccgaagaa atagccccgg agagggtcgg accaacgcaa cgctctgacc
     5041 gagaagaaga acaccgccaa acaaacccga atcccaagct tagaagcagc actttcgggt
     5101 gaaaagtggc actctacacc gacgatctgg ctcgccgagc atgacaacac cttcgattga
     5161 attcagggta atgacctccg gcgccttcac tgcggcgcat ctggcgttga tgccggagct
     5221 cgagcgtctg acgaaggcaa aggtcgtaac cgcctcgacc tccatcggca cgggcgagac
     5281 gtccatcccg aaccggctca agcgcggcga agtcgtggac atcgtcatcg ttgcggagtc
     5341 cgtgctccgt cagttcatcg aggatggcct ggtattggca gaaggccatg cggttattgc
     5401 ccgatcgagc atcgccgtgg cggtgcgcgc aggcgcgccc aaacccgata tcagtacggt
     5461 cgatgcgttg agaaggacgc tgctgcaggc aaaatccatc ggatattccg cgagcgaaag
     5521 cggcaagtac ttcagcaccg aactggttca gcgcctcggg atcgccgatc aagtgctcgg
     5581 caagagccgc ctcgtcggcg gtggcgagcg cgtaggcgcc gtggtggcgc gtggcgaact
     5641 ggagattggc ttccagcagc tcagcgagct gctgcccgtg aagggaatcg cccacatcac
     5701 actcctgccg ccggagttgc agaaagtcac gtcggttgcc gcgggcgttg ccgcgagttc
     5761 tccgaacaag gccctcgctc gctcggctat cgcattcctg gcttcgccgg aagcggcaca
     5821 ggcgattgcg aacactggcc tggaacccac ggccctgagt cggggtcgga ccacggcaat
     5881 gcattgaccg gactaaacac tatccgcgac ccgcgatccg cgagtcacca gtaaggaacc
     5941 aagaacgagg cacgcccatg cagatcctgc agccgcccgg ctggaaaaag ccaagaggct
     6001 attcaaacgg aatcgtcgcg aagggacgca ccgtcttcgt agcgggtcag gtcggctgga
     6061 acgccgatga gaaattcgaa accagcgact tcaccggcca ggcaagacag gcgttgaaaa
     6121 acatcgtcgc catcctcgcc caggccggcg caggacccga gcatatctgc cggatgacct
     6181 ggtacgtcgc cgaccgccac gagtacaacg cgagcctcaa ggggctgggc gccgcctacc
     6241 gcgaagtcat aggcaggaat tttcccgtga tgacggcgat acaggttgcc ggcttcgtgg
     6301 aggagggagc caaactggag atcgaggtta ccgcggtgat cccggaatga gtgcgcaaat
     6361 cgtgaaaaaa gggacgcacc ccttttcttc tcctatttcc gcgggccgaa gtaaataggg
     6421 gacagaccac ctttttgaca tgcgggaaac gtggtctgtc ccttatttac tgtcttcatc
     6481 caggcccgtg gcgccttgaa gaaggagctg ttgggccact tgaggcgcac acgggggatg
     6541 cgtcgctcgc gccaccaccc gcagaagacc gaagatcacg gccagatcac cgacaccgt
//
LOCUS       DBNGWZ010000035         2075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3277700, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000035 DBNGWZ010000000
VERSION     DBNGWZ010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3277700"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..990)
                     /locus_tag="AC16CX_01040"
     CDS             complement(1..990)
                     /locus_tag="AC16CX_01040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3011128.1"
                     /translation="MLDEAGHVSNPSAEAGSSDDGPLLDAYSRVVTSVAETVGPAVVR
                     LDVRRRAQEREVGGSGSGFIFTPDGLIVTNSHVVSGATAIAATLNNGQNFQADLIGDD
                     PDTDLAVVRVAGHKLPSVLLGESRNLRVGQLVVAIGNPYGFQCTVTAGVVSALGRSLR
                     SQNGRLMDDVIQTDAALNPGNSGGPLVSSRGEVIGVNTAVIMPAQGICFATAIDTAKV
                     TIAQLLKHGRVRRARLGLAGQNIVLPRRTVRHYGLERDGAVQVASIERGGPASRAGFE
                     QGDIIVGFAGQPVSGFDDLHRLLTEEKLGERVPVTVLRRGRRLKFEIQPEEFR"
     gene            complement(1128..>2075)
                     /locus_tag="AC16CX_01045"
     CDS             complement(1128..>2075)
                     /locus_tag="AC16CX_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3011129.1"
                     /translation="IGRTLETLLGGRQVTRFKREGKQYDVIVRLEDKDRVQPTDLTSI
                     YVRAKDGQLVQLSNVVNLKETVAPKELNHFNRLRAAIISASLAPGYSLSEALDFLERA
                     AREEFGPAALTQLDGQSREYRESGAQLLLIFVLALAFIYLVLSAQFESFVSPFVIMLT
                     VPLAMTGALAGLLLLDAALGTGGTLNVYSKVGLIMLVGLITKHGILIVEFANQLMARG
                     RDKFEAVKEAAALRLRPILMTTGAMVLGAVPLAIATGPGAEARRPIGWVIVGGLLFGT
                     LLTLYVIPAAYTLLVGKRRVAPGEEAGGTVAAHTPADKA"
BASE COUNT          335 a          714 c          692 g          334 t
ORIGIN      
        1 ctatcggaat tcttcaggct ggatttcgaa cttcaaccgc cgcccgcgtc gcaacacggt
       61 gaccggcacg cgctcgccga gcttctcctc ggtcagcagg cgatgcaggt cgtcgaagcc
      121 gctcaccggt tgaccggcga agccgacgat gatgtcgccc tgctcgaacc cagcgcggct
      181 cgcgggccca ccgcgctcga tcgaggcgac ctgcacggcg ccgtcgcgct cgagtccgta
      241 gtgccgcacc gtccgccgcg gcagcacgat attctgtccc gccagcccca ggcgggcgcg
      301 ccgcacacgc ccgtgcttga gaagctgcgc aatggtcacc ttcgcggtat cgatcgccgt
      361 ggcgaagcag atgccctgcg ccggcatgat gaccgcggtg ttgacgccga tcacctctcc
      421 gcgcgaggaa accagcgggc cgcccgagtt gccgggattg agggcggcgt cggtctggat
      481 cacgtcatcc atcagccgcc cgttctgcga gcgcagcgag cggccgagcg cggatacgac
      541 accggcggtc acggtgcatt gaaagccgta aggattaccg atggccacca cgagttgtcc
      601 cactcgcagg ttgcgcgatt ctccgagcag taccgacggc agcttgtgac cggcgacgcg
      661 caccaccgcg aggtcggtgt cgggatcgtc gccgataagg tccgcctgga aattctgccc
      721 attgttgagc gtcgccgcga ttgccgtcgc gccggacacg acgtggctgt tggtgacgat
      781 caggccgtcc ggcgtgaaga tgaagccgga gccgctgccg ccaacctcgc gctcctgcgc
      841 gcggcgcctg acgtcgagcc gcaccacggc ggggccgact gtttcggcaa cgctcgtgac
      901 cacgcgcgag tacgcgtcga gcagcggccc gtcgtccgaa gaccctgctt ctgccgaggg
      961 attggaaacg tgcccggctt cgtccagcat cctgcgaaga tggggacggc cggggcgcgt
     1021 atcaagcgac gcgccccctg aagggtgagg gatgagggat gacccccgca gcgcgggagt
     1081 caaaaccagg tcatccttcc gccttcatcc ttcatccttc tgtctcatca ggccttgtcg
     1141 gcgggcgtat gcgccgctac cgtgccgccg gcttcctcgc ccggcgcgac acggcgcttc
     1201 ccgacgagca gcgtgtaagc ggcgggaatc acgtagagcg tgagcagcgt gccgaacagc
     1261 aggccgccga cgatgaccca gccgatcggg cggcgtgctt ccgcgccggg ccccgtcgcg
     1321 atcgcgagcg gcaccgcgcc gagtaccatc gcgccggtcg tcatcaggat cgggcgcaac
     1381 cgcagggcag ccgcctcctt caccgcttcg aacttgtccc gcccgcgcgc catgagctgg
     1441 ttggcgaact cgacgatcag gatgccgtgc ttggtgatga gccccaccag catgatgagc
     1501 ccgaccttgc tgtagacgtt gagcgtgccg ccggtgccca gtgccgcgtc gagcagcagc
     1561 agccccgcca gcgccccggt catggcgagc ggcacggtga gcatgatcac gaacggcgag
     1621 acgaagctct cgaactgcgc ggagagcacg aggtagatga aggcgagcgc cagcacgaag
     1681 atgagcagca gctgcgcgcc tgattcccgg tactcgcgtg actggccgtc gagttgcgtc
     1741 agcgccgccg ggccgaactc ctcgcgcgcc gccctctcca agaaatccag cgcttcgctg
     1801 aggctgtatc ccggcgcgag gctcgcgctg atgatggccg cgcgcaggcg gttgaaatgg
     1861 ttgagctcct tgggcgcgac cgtttccttg aggttcacta cgttggaaag ctgcaccagc
     1921 tgcccgtcct tggcgcggac gtagatcgag gtgagatcgg tcggctgcac gcggtccttg
     1981 tcctcgaggc gcacgatcac gtcgtactgc ttgccctcgc gcttgaagcg cgtgacctgg
     2041 cgcccgccga gcagcgtctc cagcgtgcgg ccgat
//
LOCUS       DBNGWZ010000036         3186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1766629, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000036 DBNGWZ010000000
VERSION     DBNGWZ010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1766629"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16CX_01050"
     CDS             <1..1010
                     /locus_tag="AC16CX_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3011130.1"
                     /translation="LDNVFASIASGVITTDVQDTIVLCNRAATGILGRSEEQLKGQPL
                     TEALPAMGDTFTLMLNKVKGEQRPVVGHEVRPVFSDRGALNWSLNVAPLVNAAPMKKD
                     GEQGSHGLTIVVDDLTEKKRLQGKFEVFKRMVPPAVVERLNPETLALGGIRRELSIVF
                     ADIQGFTSLSETLDPEIVVKVLNRYVGTAAEAMLLNEGTVDKYIGDAVMSFFNAPDDQ
                     PDHIMRAIRAALRMRDDIYALHEVMEPQFRLSFRIAVHVGEAVVGLVGTKDRLDYTII
                     GDTVNTAKRLQENGVPGKVILSEAVYARAKDRVVVNPMDALKVKGRVQPIPVYELVNL
                     K"
     gene            1093..2550
                     /locus_tag="AC16CX_01055"
     CDS             1093..2550
                     /locus_tag="AC16CX_01055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3011131.1"
                     /translation="MLLLSWLYALTLVSLALFGFNLLLLLTLAIRHRRPLGTAEVERI
                     GRAWPPVLVQLPIFNERYVVERLIDAAAALDYPLERLTLQVLDDSTDDTSAIAKTRVA
                     HHRACGRQITYHHRADRQGFKAGALAAGLQAVSAPVEFVAIFDADFTAPPDFLRRTLP
                     AFQADPRLGLVQARWDHLNAEESALTRAQALALDGYFTVDQVARSQAGLLMNFNGSAG
                     VWRRACIEEAGGWQGDTLAEDLDLSYRAQLSGWRLDYLPEVPAPAELPTSILALKRQQ
                     FRWAVGSYQVWRKLGGQLFAARMSLFRKAQGFLHLGAYMVQPLMLAALLLSLPVVLMH
                     GHRPLNLPWLGWAGLGPVLVGLWGQITLRRDWLKRMRYYPVLVMIAIGLSLSGTLALV
                     EAFAGRRHAFVRTPKFSSANHRGSAYALPLDWTTWGELVLALYALATGLLAVELAPGL
                     APFIFLYALGFSYTAAMGFWQSGGVRQAEQAHENL"
     gene            2550..2915
                     /locus_tag="AC16CX_01060"
     CDS             2550..2915
                     /locus_tag="AC16CX_01060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3011132.1"
                     /translation="MRVIEMSRQALEPFLEREWAAENADRFAGAVAPPDWEWREYCLA
                     AHDDEELIGAAIFRVRGGVAHLKNVISSRARRSQGIGGALVDEFVRRARELGCHKLTL
                     VTCHEDKSVRFYRRHGFEI"
     gene            complement(3017..3172)
                     /locus_tag="AC16CX_01065"
     CDS             complement(3017..3172)
                     /locus_tag="AC16CX_01065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3011133.1"
                     /translation="MGLGAQLIREAERRAREAGYKKLMVISAIGTREYYRKQGFEKGE
                     LYMGKEL"
BASE COUNT          523 a         1044 c         1033 g          586 t
ORIGIN      
        1 tgttggacaa cgtgttcgct tccattgcct cgggcgtcat caccaccgac gtgcaggaca
       61 ccatcgtcct gtgcaaccgg gccgccaccg gcatcctggg ccgctccgaa gagcagttga
      121 aaggccagcc gctcacggag gcgctgccag ccatgggcga taccttcact ttgatgctga
      181 acaaggtcaa gggcgaacag cggcctgtgg tggggcacga agtccggccg gtattctccg
      241 accgcggcgc actgaactgg agtctgaacg tcgccccgct cgtcaacgcc gccccgatga
      301 aaaaagacgg cgagcaggga tcgcacggtc tgaccatcgt ggtggacgac ctgaccgaga
      361 agaaacgcct gcaaggcaag ttcgaggtct tcaagcgcat ggtccccccg gcggtcgtcg
      421 agcggttgaa cccggagacg ctcgcgctgg gcggcatccg ccgcgagctc agcatcgtct
      481 tcgccgacat tcagggcttc acctccctga gcgagaccct cgacccggaa atcgtggtga
      541 aggtcctgaa ccgctacgtg ggaacggcgg cggaagcgat gctcctgaac gagggcaccg
      601 tggacaagta catcggcgac gcggtgatgt cgttcttcaa cgccccggac gatcagcccg
      661 accacatcat gcgggcgatc cgcgccgctc tccgcatgcg cgacgatatc tacgcgctgc
      721 acgaggtcat ggagccgcag ttccggttgt cattccgcat cgcggtgcat gtgggcgagg
      781 cggtggtggg gctggtgggc accaaagacc ggctggacta caccatcatc ggggacacgg
      841 tgaacaccgc caagcgcctc caagagaacg gcgtgcccgg caaggtgatc ttgagcgaag
      901 cggtgtacgc gcgggcgaag gaccgcgtcg tcgtcaatcc catggatgct ttgaaagtca
      961 aaggccgcgt gcagcccatc ccggtctacg agctcgtgaa tctcaaatag cctcaaaccc
     1021 gcttgtcttt caaagggccc gcgccggact cggtcgcggg ttttttcttg cccgaatgtg
     1081 gagaacgatt ggatgcttct gttgagttgg ctctatgcgc tgacgctcgt cagcctcgcc
     1141 ctttttgggt tcaacctgct gctcctgctc accctggcga tccgacatcg ccgcccgtta
     1201 gggaccgccg aagtggagcg gatcggacgc gcgtggccgc cggtcctcgt gcagctccct
     1261 atcttcaacg agcgctatgt ggtcgagcgg ctgattgacg ccgccgccgc gctcgactat
     1321 cccctggagc gactcaccct gcaagtgctc gatgactcga cggacgacac gtccgccatc
     1381 gcaaaaacgc gcgtggccca tcatcgcgcg tgcggccggc agatcaccta ccatcaccgc
     1441 gccgaccgcc agggcttcaa agccggggcg ctggccgcag ggttgcaggc cgtgtccgcg
     1501 ccagttgaat tcgtcgctat cttcgatgcg gatttcaccg cgcctccgga ttttctgcgc
     1561 cgcacccttc cggcgtttca ggccgacccg cgcctcggcc tggtacaggc ccgctgggat
     1621 catttgaatg cggaagagag cgcgctcacc cgcgcgcagg cgctggccct cgacggctac
     1681 ttcacggtgg atcaggtcgc gcgcagccag gcggggctgc tgatgaactt caacggcagc
     1741 gcgggggtgt ggcggcgggc ctgcatcgaa gaggccgggg gctggcaggg cgacacgctt
     1801 gccgaggacc tcgacctctc gtatcgcgcg caactgagcg gctggcggtt ggattatctg
     1861 ccggaggtgc ccgcacccgc cgaactgccg acttcgatcc tcgcgctcaa acgccagcag
     1921 tttcgctggg cggtcgggtc gtatcaggtc tggcgcaaac tcggagggca actgttcgcc
     1981 gcgcgcatgt cgctgttccg caaggcgcag gggtttctac atctcggcgc ctacatggtc
     2041 cagccgctca tgctggccgc gctgctgttg agcctgcccg tcgtgttgat gcacgggcac
     2101 cggccattga acttgccctg gctgggctgg gccggactcg gcccagtgct cgtgggcctg
     2161 tgggggcaaa tcacactgcg ccgggactgg ctgaagcgga tgaggtacta tccggtgctg
     2221 gtgatgattg cgatcgggct gtcgctctct ggcacgctcg cgctggtcga ggcgttcgcg
     2281 gggcggcgtc acgcgttcgt gcgcacgccc aagttctcgt ccgcgaatca ccgcgggtcc
     2341 gcctacgcgc tcccgctcga ctggacgacg tggggtgaac tcgtcctcgc cctctacgcg
     2401 ctggcgacgg gcctgctcgc cgtcgagctc gcccccggcc ttgcgccgtt catctttcta
     2461 tatgcgcttg gcttcagtta taccgcggcc atgggcttct ggcagagcgg aggggtcagg
     2521 caggcagaac aggcgcacga gaacctgtga tgcgcgtgat cgagatgagt cgccaggccc
     2581 tcgagccttt cctggagcgc gaatgggctg ccgaaaacgc cgaccgcttt gcgggtgcgg
     2641 tcgcgccgcc ggactgggaa tggcgcgaat actgcctggc cgcgcacgac gacgaggaac
     2701 tcatcggcgc cgccatcttt cgcgtacggg gtggtgtggc gcacttgaag aacgtcattt
     2761 ccagccgcgc gcggcgcagc cagggcatcg gcggcgcgct ggtggacgaa ttcgtgcggc
     2821 gtgcgcgcga actgggctgc cacaagttga cgctcgtcac gtgtcacgaa gacaaatcgg
     2881 tgcgcttcta ccggcggcac ggctttgaga tctaggccgt cctgcacaac gatgcctttc
     2941 acgttcaccg ctgccagatg gcgaggtttc tcgaccgggg ttagcgaaga gggccgatcc
     3001 ataggcccgc ccgttcctac agttccttcc ccatatacag ctcccccttc tcaaacccct
     3061 gcttccggta atactcgcgc gtcccgatgg ccgagatcac catcagtttt ttgtacccgg
     3121 cctcgcgcgc gcggcgttcg gcctcgcgga tcaactgggc gcccagcccc agatgctggg
     3181 cctcgc
//
LOCUS       DBNGWZ010000037         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_633348, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000037 DBNGWZ010000000
VERSION     DBNGWZ010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_633348"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16CX_01070"
     CDS             complement(<1..707)
                     /locus_tag="AC16CX_01070"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3011134.1"
                     /translation="MDTSYLSTTRAVYKEAALNPQQGLCCTSTPVWQLPELAIPQKML
                     AMNYGCGSTVHPRDLANAPTILYVGAGGGMELLQFAYFSRRKGGVIGLDVVDEMLVAC
                     RANLREAELQNPWFKSSFVDLRKGDALELPISDAAVDVAAQNCLFNIFHDRDLERALS
                     EMYRVLKPHGRLILSDPVCEAEIPAHLRNNEKLRALCLTGAISLKDYVKRLTDVGFGT
                     IEVRARRPYRVLSPRHYP"
     gene            complement(721..1863)
                     /locus_tag="AC16CX_01075"
     CDS             complement(721..1863)
                     /locus_tag="AC16CX_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010121989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3011135.1"
                     /translation="MLVTALLFLTALFVAYANGANDNFKGVATLYGSGAATYKSAIAL
                     ATVTTFAGSLCSVYFAAALVQAFSGNGLVPAAIAASPDFLLAVATGAGATVLLATVLG
                     LPVSTTHALTGALTGTGLLAVGTQFDFMHLGSGFFVPLLLGPVLAVLLTIPFYRALRA
                     LARRFDIKRESCICVGAREFIPVRQLQYNTVIQRFTLPAPATVGIAVGTRDDCVERYQ
                     GRLLGVSAHGMVNALHYLSAGIVSFARGLNDTPKIIGLLLLVQALNVEFGIFAIATAM
                     ALGGLLNARKVAITMSQRISRMNEGQALAANMVTGLLVILASRFGLPVSTTHVSVGAI
                     SGIGLMNGSADKGVMGGILASWLLTLPVAAIIGALVYAVLGFFPLN"
     gene            complement(1890..>2270)
                     /locus_tag="AC16CX_01080"
     CDS             complement(1890..>2270)
                     /locus_tag="AC16CX_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HIR3011136.1"
                     /translation="HLGERYGVVFNRLYVLTNMPIQRFGSMLVSKGRFEEYMALLKDA
                     YQPHNLESVMCRTLLSVDWRGYVYDCDFNQMLGLPLAWRGRPRAHLRDLIGADLKGNP
                     IVVKDHCYGCTAGQGSSCGGALVT"
BASE COUNT          444 a          720 c          740 g          366 t
ORIGIN      
        1 gggtagtggc gcggcgacag gacgcggtac ggccgcctgg cgcgcacctc gatggtgccg
       61 aaacccacgt ccgtcaggcg cttgacgtag tccttgaggg aaatcgcccc cgtcaggcac
      121 agcgcccgca gcttttcgtt gttcctcaag tgcgcgggta tctcggcttc gcacaccgga
      181 tcggagagga tcagcctgcc atgcggcttc agcacgcggt acatctccga cagcgcgcgc
      241 tccaggtcgc ggtcgtgaaa gatgttgaac aggcagttct gcgccgccac atccacggcg
      301 gcgtcgctga tgggaagctc gagggcgtcg cccttgcgca gatccacgaa ggaggatttg
      361 aaccacgggt tctgcagctc ggcctcccgc agattagcgc gacacgccac aagcatttca
      421 tcaacgacgt ccaggccgat cacgccgccc ttgcggcggg agaaataggc gaactgcagc
      481 agctccatcc cgccgccggc gccgacatag agtatggtcg gggcattggc gagatcgcgc
      541 gggtgcaccg tgctgccgca gccatagttc atggcgagca tcttctgggg gatcgccagc
      601 tcgggaagct gccagaccgg cgtggaggtg cagcacagtc cctgctgcgg attgagcgcg
      661 gcttccttgt agacagcgcg cgtcgtggac aggtacgaag tatccatgtt tcgctccgta
      721 tcaattcaaa gggaaaaatc cgagcacggc gtaaaccagc gcgccgatga ttgccgcgac
      781 cggcagcgtc agcaaccagg aggcgaggat gccgcccatt acgcccttgt cggcggatcc
      841 gttcatgagg ccgattccgc tgatcgcgcc cacggaaaca tgggtggtcg agaccggcag
      901 accgaagcgg ctcgcgagga tcaccagcaa tccggtcacc atattcgcgg ccagcgcctg
      961 gccctcgttc atgcgcgaga tccgctggct catggtgatg gcgaccttcc tggcgttgag
     1021 caggccacca agcgccatgg ccgtcgcgat cgcgaatatg ccgaactcga cgttcaacgc
     1081 ctggacgagc aggaggaggc cgatgatctt cggcgtgtcg ttgaggcctc tcgcgaagct
     1141 cacgatgccg gcactcagat aatgcaacgc atttaccatc ccatgcgcgg agacaccgag
     1201 aagcctgccc tggtacctct caacgcaatc atcgcgcgtg ccgacggcga tgccgacggt
     1261 tgccggcgcg ggcagggtga accgctggat gaccgtgttg tactgcagtt gccggaccgg
     1321 tatgaattcc cttgcgccga cgcaaatgca gctttcccgc ttgatatcga acctccgggc
     1381 aagcgcgcgc agcgcccggt agaacgggat ggtgagcagc acggcgagga cgggacccaa
     1441 cagaagcgga acgaaaaaac cggagcccag atgcatgaaa tcaaactgag tcccgacggc
     1501 cagcaaaccc gtgccggtca gggcaccggt cagagcatgg gtcgtggaga ccggcagccc
     1561 cagcaccgtc gccagaagaa ccgtcgcgcc ggcgcccgtg gccaccgcca ggagaaaatc
     1621 cggcgatgcc gctattgccg ccggcaccag cccgttgccc gaaaacgcct gcaccagcgc
     1681 cgctgcgaag tacaccgagc acagcgagcc ggcgaacgtc gtgacggtgg ccagcgcgat
     1741 ggcgctcttg taggtggctg cgccgctgcc gtagagggtc gcgacgcctt tgaagttgtc
     1801 gttcgcgccg ttggcatagg cgacgaacag ggcggtcagg aagagcaggg cggtaacaag
     1861 cacggtttta cccgtcggag gatcgcgtat caggtgacga gcgcgccgcc gcagctcgat
     1921 ccctgcccgg cagtgcagcc gtagcagtgg tctttcacca cgatcggatt gcccttgagg
     1981 tcggcgccga tcaggtcgcg caggtgcgcg cgcggccggc cgcgccacgc gagcggtagc
     2041 cccagcatct ggttgaagtc gcagtcgtag acgtaaccgc gccagtccac cgagagcagc
     2101 gtgcggcaca tcaccgattc gaggttatgc ggctggtaag cgtccttgag cagcgccatg
     2161 tactcctcga accggccttt cgagaccagc atgctgccga agcgctggat cggcatgttg
     2221 gtgagcacgt agagccggtt gaaaaccacc ccgtaccgtt ctccgaggtg
//
LOCUS       DBNGWZ010000038         8121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2018798, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000038 DBNGWZ010000000
VERSION     DBNGWZ010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2018798"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16CX_01085"
     CDS             <1..233
                     /locus_tag="AC16CX_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3011137.1"
                     /translation="AILRANGIRRLYLSGVSTSGAVLSGAKDGHDRDYDVYVIEDCCC
                     ALSEEQHQAVVEQMKRMTTILTAAEVTFSEGT"
     gene            310..1302
                     /locus_tag="AC16CX_01090"
     CDS             310..1302
                     /locus_tag="AC16CX_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011138.1"
                     /translation="MKFLRSSRMAARCAAAALAVLVTAPAVAQVTGYPNRAIRWIVPL
                     PPGGGADIVARTIAERLSKNLGQQVVVDNRPGGGTVIGAELAARSAPDGYTLLLGTAT
                     THAINASLVRKLPYDPIKDFSPISLVAILPQIIVAHPSLPAQTLKELIALARKRPGEI
                     NFASTGNGSANHLGGEMLKSVARVNLVHVPYKGASPAVTDLVAGNVQFMFSTIPPVLP
                     HVKSGRLKALAVAQARRSTLLPELPTTAENGAPGVEAASWNGVLVPAGTPNEIIAKLH
                     GDLAAVMNLADVRARLATSGVEPQINTPAEFAAYIRSETARYARVVKESGARLD"
     gene            1411..1484
                     /locus_tag="AC16CX_01095"
     tRNA            1411..1484
                     /locus_tag="AC16CX_01095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1445..1447,aa:Ile,seq:cat)
     gene            complement(1486..2505)
                     /locus_tag="AC16CX_01100"
     CDS             complement(1486..2505)
                     /locus_tag="AC16CX_01100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3011139.1"
                     /translation="MKRRGRGIACMWYPIGFTVSANPSAAVVKVNEDGTATLLTGTVE
                     TGQGSLTVLGQIAAEELGIATDDVHVVSADTDAMPMDTGAIASRTTYVTGNAVRLAAE
                     KAKAVLFEAAAPMLGVKPEQLEARDRKIQVKSFPQRNLPIGDVAHHAQVVKGQPPIGS
                     ASWNPPTVALDPETGQGKPFSTYVYATQIAEVDVDDETGEVEVLRVVAAHDCGTPINP
                     MLVEGQVEGGIAMGIGFALQEEMLFDDRGRHVNPSLTNYIVPTSLDLPEIEVDIVPSY
                     DPTGPFGAKGVGEPTSVPTAAAILNAIHNAVGVRITSLPATAEKVLAAIKARRAAGSA
                     RIESR"
     gene            complement(2502..3902)
                     /locus_tag="AC16CX_01105"
     CDS             complement(2502..3902)
                     /locus_tag="AC16CX_01105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3011140.1"
                     /translation="MADRRKTGQKLRRPPLETPRVANEAVRLPALLPQVPYPIETLVP
                     QARQQTPAVGQRVTRVDGRLHGLGQTRYIDDLAFPGMLHARIKRAGIASARIRRIDTS
                     AAEALPGVMAVLTGAEVPVNSFGPSIQDQPVLAHERVFHAGDGVAAVAALTEQVAADA
                     LEKIVVEYEPLPAVFDPLEALGEDAPRVHPPSSNIYATKVIRKGDVERGFAASDRIFE
                     GRFRTQMVEHVALEPHAAIAEWDASGRVTIWSTLGRITLARADVARTLKLPINRVRMV
                     GTIVGGNFGDKNEITQEPVLALLSKKTGRPVKGVYTREEEFTSTTTRHPLVMDYRTGV
                     TKQGRILARTVRLVLDGGAYCSWSETTLGKAAILAPGPYDIENLRVEAFVVYTNKTMT
                     GAMRGFGAPQVCFAYESHMDDIATALGIDALDIRLLNAFSEGSVSPTGQVLHSVVVKE
                     SLAKAAERYGWREGRP"
     gene            complement(3915..4787)
                     /locus_tag="AC16CX_01110"
     CDS             complement(3915..4787)
                     /locus_tag="AC16CX_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3011141.1"
                     /translation="MAEAQKKYRFLFIQPFELPRGSRFADRYHSDALSKEQRMRMDHL
                     NIRHLLEDVEWDFHGGPIAPYGDWAVETREEFCHVAAARLPIVREACASGKYNAIVLL
                     GGGEPGFLESREIARRYGIAVSSCAFSQMHIATMLGNKFSVIDFAESHNMYYHDLVVR
                     HRMAERCASIRSVNYYHARPGHADASTMADERDKARNNERSEAVERALTEAVAAIEED
                     GAEVITFGCSDAFWLQPFLQQRLGTMGWEVPVLEGYSCAIALAKLYVDLGVDASGLMF
                     PGDRPQRWRRKKVF"
     gene            complement(4801..5931)
                     /locus_tag="AC16CX_01115"
     CDS             complement(4801..5931)
                     /locus_tag="AC16CX_01115"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3011142.1"
                     /translation="MNAPARVGELRVPQRMKAWVLGGPGELHLTEKPVPQPGTAEVLV
                     RIDATAVCGTDLEVISHGAPALVQGGPPFNKNFTPGHEYMGTVAGLGPGVDEFEVGDR
                     VAVEVHAGCGRCERCREGMYTACINYGMNYGDRDKGHRANGFTTDGGFAQYAVNHVNT
                     LVPVPGDMSDEEATLIVTAGTAIYGLDVLGGLVAGQSVVVTGPGPIGLMGVGVAKALG
                     ADPVVLTGTRADRTRLEIGRKLGADEIVDVGSEDAVRAIMRITRGRGAHYVLECSGAP
                     NALNEAVHMVSRGGRICLVAFPHGSAPVDVGRLVSDNIYVFGIRGEGRSATRRAAALM
                     AQKRFPAKLLHTHTFPLEQVPTAIRYAAERIEDAIKVVVKIH"
     gene            complement(5928..6428)
                     /locus_tag="AC16CX_01120"
     CDS             complement(5928..6428)
                     /locus_tag="AC16CX_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3011143.1"
                     /translation="MKISLTVNGETRSASVPPETTLLALLRDVLHLTGAKLGCDVGDC
                     GACTVLVDGRPVNSCLMLAGQADGRDVLTIEGLATRDGLHPLQQAFEAHASLQCGFCG
                     PGVILAAKALLDMNPRPGVPEIRDALAGNLCRCTGYTKMINAVQEVARVVRGEGPDSP
                     AGTERI"
     gene            complement(6425..7318)
                     /locus_tag="AC16CX_01125"
     CDS             complement(6425..7318)
                     /locus_tag="AC16CX_01125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3011144.1"
                     /translation="MKPFDFVVAHDSTEALAALARAGPDARVVAGGTDLLVELKSAAR
                     VPALVVDISRARDLKRIAMTDEGIVIGAAVTHDEIMRSPLIQKWLPAVADAAHTIGAV
                     QTRSLGTLGGNLVTCVPSMDGGPVLVALDAQVTIAGPAARRRIPLVEFFVGPRKTALG
                     PGELLLEIVIPKDSLGKGADFQKFGLRKGQALALVNVAAAFGVERQRNAFVEPCIALG
                     AVAPTVIRATRAEAYLEGRAISNEAMREAGRIAATEARPITDFRASAGYRRDLIAVLT
                     RRALQNAEARAAARKPADAPS"
     gene            complement(7471..7836)
                     /locus_tag="AC16CX_01130"
     CDS             complement(7471..7836)
                     /locus_tag="AC16CX_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3011145.1"
                     /translation="MQRRASVENVEGLGINVSRLFTITFIIAGTLFGLAGGLHAPLIR
                     VDPQMSLEVLAFVFMVVIIGGLRSIKGTLVSALIVGQVVSLGSLIYAALVQMAPFAIM
                     PLILLIKPTGLYGGAMLKR"
     gene            7881..>8121
                     /locus_tag="AC16CX_01135"
     CDS             7881..>8121
                     /locus_tag="AC16CX_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011146.1"
                     /translation="MRKTGLMILPWLIAAAAFAQAPVEDLTSREQAIQRSQQRTGAAY
                     RELQQAQYEAKLAEQDFLNAQEAHGAAQKQADEMKR"
BASE COUNT         1276 a         2827 c         2690 g         1328 t
ORIGIN      
        1 aagcgatcct gcgcgcgaac ggcatacgcc gtctttatct ttccggggtt tcgaccagcg
       61 gagcggtgct gtccggcgcg aaggacgggc acgaccgcga ctacgacgtg tacgtgatcg
      121 aggattgctg ctgcgcgttg agcgaagaac agcaccaggc ggtcgtcgag cagatgaagc
      181 gcatgacgac gatcctgacc gcggccgagg tgacattttc cgagggcacg tagtccggtc
      241 gtccgggccg acccgaggcg ggcgggcacg aagaacaacg gcgccgccgg gctcaacgaa
      301 ggaggaacga tgaaattctt gcgctcatcc aggatggccg cccggtgtgc ggccgccgcg
      361 ctggctgtgc tcgtgaccgc gcctgccgtc gcccaagtca ccggttatcc caatcgcgcg
      421 attcgctgga tcgtgccgct gccgcccggg ggcggcgccg atatcgtcgc gcgcacgatt
      481 gccgaacggc tctcgaaaaa cctcggacag caggtggttg tcgataaccg gccggggggc
      541 ggcacggtga tcggcgccga gctcgccgcg cggtccgcgc cggacggtta cacgctgcta
      601 ctcggcaccg ccacgacgca cgccatcaac gcgagccttg tcaggaagct gccgtacgac
      661 ccgatcaagg atttctcccc gatatcgctg gtagcgatcc tgccccagat catcgtggcg
      721 cacccgtcgc tgccggccca gacgctcaag gagctcatcg cgctcgcgcg caagcggccc
      781 ggcgagatca atttcgcgtc gaccggcaac ggcagcgcca atcacctcgg cggcgagatg
      841 ctgaagagcg tcgccagggt gaacctcgtg cacgtgccgt acaagggcgc gtctcccgcg
      901 gtcaccgacc tggtagcggg caacgtccag ttcatgttct cgaccattcc gcccgtgctg
      961 ccgcacgtga aatcggggcg cctgaaagcg ctggcggtcg cccaggccag gcgctcgacg
     1021 ctgttgcccg aactgccgac gacggcggag aacggagcgc ccggcgtgga agcggcttcg
     1081 tggaacggcg tgctcgttcc cgccggcacg ccgaacgaga tcatcgcgaa gctgcacggc
     1141 gacctggcgg cggtgatgaa tctcgcggac gtgcgcgcgc ggctcgccac gagcggcgtg
     1201 gagccgcaaa tcaacacgcc ggctgaattc gccgcctaca tccggtcgga aaccgcgcgc
     1261 tacgccaggg tcgtcaagga atccggcgcg cgattggact gaagcgcatc cgtcgttgcg
     1321 cacggcctgt gccgtgccgt gaatgcccgg gcggccggct tgtggcgccg gatatcccgc
     1381 catcggcgaa aatcgtaaaa tcgcgcctca gggtctgtag ctcagccggt cagagcaggg
     1441 gactcataat cccttggtcg ttggttcgaa tccaaccaga cccactcaac gggactcgat
     1501 gcgtgccgag ccggccgcgc gccttgcctt gatggcggcg agaactttct ccgccgtggc
     1561 cggcagcgag gtgatgcgca cgccgaccgc gttgtggatc gcgttcagga tcgccgcggc
     1621 ggtgggcacc gacgtcggct cccccacgcc cttggcgccg aacggcccgg tcggatcgta
     1681 gctcggcacg atgtccacct cgatctcggg caggtcgaga ctggtgggca cgatgtagtt
     1741 ggtgaggctg gggttgacgt gccggccacg gtcgtcgaac agcatttcct cctgcagcgc
     1801 gaagccgatt cccatcgcaa tgccgccctc gacctggcct tccaccagca tcgggttgat
     1861 cggcgtgccg cagtcgtgcg cggcgacgac gcgcagcacc tcgacttcgc cagtctcgtc
     1921 gtccacgtcc acttcggcga tctgcgtggc gtaaacgtac gtgctgaagg gcttgccctg
     1981 cccggtctcg ggatcaagcg ccacggtcgg aggattccac gacgcgctcc caattggcgg
     2041 ctgtcccttg acgacttgcg cgtgatgcgc cacgtcaccg atgggcaggt tgcgctgcgg
     2101 gaagctcttg acctggatct tgcggtcgcg cgcttccagc tgctcgggct tcacgccgag
     2161 catcggcgcg gcggcttcga acaggaccgc cttcgctttt tcggccgcga gccggaccgc
     2221 gttgccggtc acgtacgtgg tgcggctcgc gatggcgcca gtatccatcg gcatcgcatc
     2281 cgtgtcggcc gaaacgacgt gcacgtcgtc ggtggcgatg cccagttcct ctgccgcgat
     2341 ctggcccagc acggtgagcg agccctgtcc ggtttccacc gtcccggtga gcagcgtcgc
     2401 ggtcccgtct tcgttgacct tcaccaccgc cgcgctcggg ttggcggaaa cggtgaagcc
     2461 gatcgggtac cacatgcagg cgatgccgcg tccgcgccgc ttcatggccg cccctcccgc
     2521 cagccgtagc gctccgccgc cttggcgagc gactccttca ccacgacgct gtgcagaacc
     2581 tggccggtgg ggctcaccga gccttcgctg aaggcgttga gcagccggat atcgagcgca
     2641 tcgattccga gcgcggtcgc gatgtcgtcc atgtgcgact cgtaggcgaa acacacctgc
     2701 ggcgcaccga aaccccgcat ggcgcccgtc atcgtcttgt tggtatagac gacgaacgcc
     2761 tcgacgcgca ggttctcgat gtcgtagggg ccgggcgcga ggatcgccgc cttgccgagg
     2821 gtcgtctcgc tccacgagca gtaggcgccg ccatccagca cgagccgcac cgtgcgcgcg
     2881 aggatcctgc cttgcttcgt caccccggtg cggtagtcca tcacgagcgg atggcgcgtt
     2941 gtcgtcgagg tgaattcctc ctcgcgcgtg tagacgccct tcaccggccg gccggtcttc
     3001 ttcgacagca gcgcgagcac cggttcctgg gtgatctcgt tcttgtcgcc gaagttgccg
     3061 ccgacgatgg tgccgaccat gcgcacgcgg ttgatcggga gcttcagcgt gcgcgcgacg
     3121 tccgcgcgcg cgagcgtgat ccgcccgagc gtggaccaga tggtgacgcg gccgctcgcg
     3181 tcccactcgg cgatcgccgc gtggggctcg agcgccacgt gctccaccat ctgggtgcgg
     3241 aagcggccct cgaaaatgcg gtccgaggcg gcgaatccgc gctcgacgtc gcccttccgg
     3301 atcaccttcg tcgcgtaaat gttgcttgag ggcgggtgca ccctgggcgc atcctccccg
     3361 agcgcctcaa gcggatcgaa caccgcgggc agcggctcgt attccaccac gattttctcg
     3421 agcgcgtcgg cggcgacttg ttcggtcagg gcggcgacgg cggcgacccc gtcgcccgcg
     3481 tgaaacacgc gctcgtgcgc gagcaccggc tggtcctgga tcgaggggcc gaacgagttg
     3541 accggcacct cggcccccgt cagcacggcc atcacgcccg gcagggcttc cgccgcgctc
     3601 gtatcgatgc gacggatgcg ggccgaggcg atgcccgcgc gcttgattct cgcgtgcagc
     3661 atgccgggga aagcgaggtc gtctatgtag cgggtctggc cgagcccgtg caggcgcccg
     3721 tccacccgcg tcacgcgctg gccgacggcg ggggtctgct ggcgcgcctg cggcaccagc
     3781 gtctcgatcg ggtacggcac ctgcggcagc agcgccggaa gccgcaccgc ttcattggcg
     3841 acgcgcggcg tctcgagcgg cggccgccgc agcttttgcc cggtttttct cctgtcagcc
     3901 acgaccaccg ccgctcagaa gaccttcttc cggcgccagc gttgcgggcg gtcgcccggg
     3961 aacatgagcc cgctcgcgtc cacgcccagg tccacataca gcttcgccag cgcgatcgcg
     4021 cagctgtagc cctccagcac cggcacttcc cagcccatcg tacccaggcg ctgctgcagg
     4081 aacggctgca gccagaacgc gtccgagcaa ccgaaggtga tcacctcggc gccgtcctcc
     4141 tcgatcgcgg cgaccgcttc cgtgagcgcg cgctccacgg cctcggagcg ctcgttgttg
     4201 cgggccttgt cgcgctcgtc agccatcgtg ctcgcgtccg cgtgccccgg ccgcgcgtgg
     4261 taatagttga cgctccggat cgacgcgcag cgctccgcca tccggtgccg cacgacgaga
     4321 tcgtggtaat acatgttgtg cgactcggca aaatcgatga cgctgaactt gttgccgagc
     4381 atcgtcgcga tgtgcatctg ggagaacgcg caggagctca ccgcgatccc gtaccggcgc
     4441 gcgatctccc tcgattccag gaagcccggt tccccgccgc cgagcagcac gatggcgttg
     4501 tacttgccgc tcgcgcaggc ctcgcgcacg atcggcaggc gcgccgccgc gacgtggcag
     4561 aattcctcgc gcgtctccac cgcccagtcg ccgtagggcg cgatcggccc gccgtggaaa
     4621 tcccattcga cgtcctcgag caggtgccgg atgttgagat ggtccatccg catgcgctgc
     4681 tccttcgaca gcgcgtcgga atggtagcgg tccgcgaacc ggctgccgcg gggcagctcg
     4741 aacggctgga tgaacaggaa ccggtatttt ttctgcgcct ccgccacggg tcctccgttc
     4801 ctagtggatt ttcaccacga ccttgatcgc gtcctcgatg cgctcggcgg cgtagcggat
     4861 cgccgtcggc acctgctcca ggggaaaggt atgcgtgtgg aggagcttcg ccggaaagcg
     4921 cttctgcgcc atcagcgccg ccgcccggcg cgtcgcgctc cggccctcgc cgcggatccc
     4981 gaacacgtag atgttgtcgc tgacgaggcg cccgacgtcc accggcgctg atccatgcgg
     5041 gaacgccacc aggcagatgc ggccgccgcg gctgaccatg tgcaccgcct cgttgagcgc
     5101 gttgggcgcg cccgagcact cgagcacgta gtgcgcgccc cggccgcggg tgatgcgcat
     5161 gatcgcccgg accgcgtcct cgctgcccac gtccacgatt tcgtcggcac cgagcttgcg
     5221 cccgatttcc agccgcgtgc ggtccgcgcg cgtgccggtc aggaccacgg ggtcggcccc
     5281 gagcgccttc gcgacaccca cgcccatcag cccgatcggc ccggggcccg tcaccaccac
     5341 cgactggccg gcgacgagcc ctccgagcac atcgagcccg tagatcgcgg tgccggcggt
     5401 cacgatcaac gtggcttcct cgtcgctcat gtcgccggga acgggcacga gcgtattcac
     5461 gtggttcacg gcatattgcg cgaaaccccc gtcggtggtg aaaccattgg cgcggtggcc
     5521 cttgtcacgg tcgccgtagt tcatgccgta gttgatgcag gcggtgtaca tcccctcgcg
     5581 gcaacgctcg cagcgtccgc aaccggcgtg aacctcgacc gcgacgcggt caccgacttc
     5641 gaactcgtca acacccgggc ccaaccccgc cacggttccc atgtattcat gcccgggcgt
     5701 gaagttcttg ttgaacggcg gcccgccctg caccagcgcg ggcgccccgt ggctgatgac
     5761 ctcgagatcg gtgccgcaga cagcggtcgc atcgatgcgc accagcactt cggctgtgcc
     5821 gggctgcggc acgggctttt cggtcaggtg cagctcaccc ggtccgccga ggacccaggc
     5881 cttcatccgc tgcggcaccc gcagttcgcc cactcgcgcc ggtgcgttca tatccgctcc
     5941 gttcccgccg gcgagtccgg cccttccccg cgcacgacgc gcgccacctc ctgcaccgcg
     6001 ttgatcatct tcgtgtaacc ggtgcagcgg cacaggtttc cggcgagcgc atcgcggatc
     6061 tccggcacgc ccggccgcgg gttcatgtcg agcagcgcct tggcggccag aatcacgccg
     6121 ggtccgcaga aaccgcactg cagcgaggcg tgcgcctcga atgcctgctg cagcgggtgc
     6181 aatccgtcgc gcgtcgcgag cccttcgatc gtcagcacgt cgcggccgtc ggcctggccc
     6241 gcgagcatca ggcacgaatt gaccgggcgg ccgtccacga ggacggtgca ggcgccgcaa
     6301 tcgcccacgt cgcaaccgag cttggcgccg gtgaggtgca acacatcgcg cagcagcgcg
     6361 agcagcgtcg tttcgggcgg aaccgacgcc gaccgcgtct cgccgtttac cgtcaaggag
     6421 attttcatga gggggcgtcg gcgggtttgc gtgcggccgc gcgcgcctcg gcgttttgca
     6481 gggcgcgccg ggtgagcacc gcgatcaggt cgcgccggta accggccgat gcccggaaat
     6541 cggtgatcgg ccgcgcttcg gtcgccgcga tccggcccgc ctcgcgcatc gcttcgttcg
     6601 atatcgcccg gccttcgaga taggcctccg cccgcgtcgc ccgaatgacc gtcggcgcga
     6661 cggcgccgag cgcgatgcac ggctcgacga aggcgttacg ctgccgctcc acgccgaagg
     6721 ccgcggcgac gttgaccagc gccagcgcct gtcccttgcg caatccgaat ttctggaaat
     6781 ccgcgccctt gccgagactg tctttcggga tcacgatctc cagcagcagc tcgccaggcc
     6841 cgagcgccgt cttgcgcggg ccgacaaaaa actccacgag cgggatacgg cgccgggcgg
     6901 cggggcccgc gatcgtgact tgcgcgtcga gtgcgaccag caccgggccg ccgtccatcg
     6961 acggcacgca ggtcaccaga ttgccgccga gcgtgccgag gctgcgggtc tgcaccgccc
     7021 cgatcgtgtg ggcggcgtcg gcgacggccg gcagccactt ctggatcagc ggcgaacgca
     7081 tgatttcgtc gtgggtgacg gcggcgccga tgacgatgcc ctcatcggtc atcgcgatgc
     7141 gcttgaggtc gcgcgcgcgg gagatatcga cgacgagcgc cggcacgcgc gccgccgact
     7201 tgagctcgac cagcaaatcc gtgccgccgg cgaccacgcg ggcatccggt ccggctcgag
     7261 cgagcgcggc aagcgcctcg gtggaatcgt gcgcgacgac gaaatcgaac ggcttcatcg
     7321 acctccgcga gggttcgagg cgcgccggac gtccccacgt agcttccggc gaattgaaaa
     7381 agtgtccggt atcagcccga tccgctgatt gacccagatc aaagaggatc attccccgcg
     7441 ggcttgaagc ttcgggaatt ctccctgccg ttaccgtttc agcatcgcgc cgccatacag
     7501 cccggtcggt ttaatcagga gaatcaacgg catgatggca aacggcgcca tctgcacgag
     7561 agccgcgtag atcagggacc ccagggagac gacctgtccg acgatcaggg ccgacaccag
     7621 ggttcccttg atgcttctca gaccgccgat gatgactacc atgaaaacga acgccagcac
     7681 ctccaggctc atctggggat ccaccctaat cagcggcgca tgcaggcctc cggccagtcc
     7741 aaacagggtg cccgcgatga tgaaggtgat ggtgaagagc cgggaaacgt tgatgccgag
     7801 gccttcgacg ttttcgacgc tcgcgcgccg ttgcatggat gcggtggtac cattactgcg
     7861 gaacaacgag catttcgcgt atgcgcaaga cgggcctgat gatcctgcca tggttgattg
     7921 cggcggcggc tttcgcgcag gcgccggtcg aggatctgac gagccgggag caggcgatcc
     7981 agcgcagtca gcaaaggacc ggcgccgcgt accgcgagct gcagcaggcg cagtacgagg
     8041 cgaaactcgc cgagcaggat tttctcaatg cgcaggaagc gcacggcgcc gcgcagaagc
     8101 aagccgacga gatgaaacgg c
//
LOCUS       DBNGWZ010000039         5878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3152168, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000039 DBNGWZ010000000
VERSION     DBNGWZ010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3152168"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="AC16CX_01140"
     CDS             complement(<1..806)
                     /locus_tag="AC16CX_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR3011147.1"
                     /translation="MADTTRIPGMGWYWGAAAIVLATLVAVGGIAVWLFLEYEAAVPF
                     LAPGRHTVELKKPGSYLVWNDFRTVFKGRSYDEPRHLPHGAQISVIDKSSGQALSVGS
                     YSGATSTTGSTESIAVVQFSITQPGLYEIVVAGDFPQRVFSVGRNLVFHIVGAVSGAI
                     ALVFVGFGAALAVAGWAFLRREEARAAAARAHPTPTSSPVASAAPGEQSLKRITAIVY
                     GLQIAAFLVGITFIAAVIVNYVKRRDVEGTWLESHFRWQIRTFWYGLLWS"
     gene            complement(832..2175)
                     /locus_tag="AC16CX_01145"
     CDS             complement(832..2175)
                     /locus_tag="AC16CX_01145"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3011148.1"
                     /translation="MFAKRNAVVETPADAAASAGGGVLIYAGFWARVAALLVDSAIVM
                     IFGIILFIVLGMAMGGTGLAIGYVIFFFVQLLYWPVLESSARQATFGKQLLGIQVTDA
                     DGNRTSFIRALLRNLAKIISSIPLGIGFLLAAFTSRKQALHDMITSCLVVRAGPSSFL
                     KAVVAAVGGLVIAAGASYYYVTEVYLPQLTKDMGREMEKAMKGAMKGQPGMKAVPARP
                     PVAKAPAAATKPPVARAAPAVSAPPAAGPATQIAAVTPSAPPATAPTPAPAPKEATPA
                     VKPAATAPAKPAAAPMAAQATPVKPAAAPVKKAEAAPKPAPEAAPSADEKPKAKRRVA
                     KPKPAAPAETAAAPPMAPARTPKYNDVMTAVMYRDRAGAAELLELGWWVDRPDSNGVT
                     PLMAAAMNGDASIVQLLLKHGADPNRSGPGGSVLAYAAQGRDAKVIELLKRAGTR"
     gene            2426..3154
                     /locus_tag="AC16CX_01150"
     CDS             2426..3154
                     /locus_tag="AC16CX_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3011149.1"
                     /translation="MPKTPLGRLPREAIRMLELPPLQKEILDGFKALVDLTGTVSDAM
                     DELGIVGVVPAYVLPPVTTGKSIVGPALTVRNIQRTAQIHKAALDKTNTQGESEAHNL
                     AESGDVLVIEGVTGCSNMGGQSATIARRQGFIGAVVDGTVRDPQQYREMGWPVWCRGF
                     TPVTGKWRMQTVEINGTVQIAGVQVKPGDLVCADEAGVAFVPREQAAAVLEAARKIDT
                     GDTRRKKDIDSGASVADLITRKYK"
     gene            3175..4641
                     /locus_tag="AC16CX_01155"
     CDS             3175..4641
                     /locus_tag="AC16CX_01155"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3011150.1"
                     /translation="MSDIKPSNLLFILSDEHNKRVLGCYGHPMIQTPNLDALAARGTR
                     FSNAYTNCPICVPARASFATGRYVHDVRCWDNAIAYTGQAPSWGHRLMEQSHRVVSIG
                     KLHYKESKPEVNGFDEEILPLHIVNGMGDLLGLIRDELPRRPGSLKLGPEAGPGESEY
                     TRYDRSIADETVKWLNSEAARRRDKPWALYVGFVSPHFPLIAPPRFYDLYPENKVPWP
                     DLYDPAERPRHPFLDAMRKCLCFDEPFDPPMVRRAIAAYFGLVSFLDDNIGKILRALE
                     ETGLAKSTRVVYSSDHGDNLGTRGLWGKSTMYEESAGIPLIMAGPDVPAGGTCDTPVT
                     LADCFPTFIHALGAKPDPRDRDLPGVSLLGIARGAVPQRTIFSEYHAAGAITASYMIR
                     HGRYKYIHYVGMPPMLFDLEADPHERRDLACESAYEKIIAECEGLLRKIVDPEAVDKL
                     ARADQKAHIEKNGGEEAILKKGTFRYSPPPGAEATYYY"
     gene            complement(4752..5726)
                     /locus_tag="AC16CX_01160"
                     /pseudo
     CDS             complement(4752..5726)
                     /locus_tag="AC16CX_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
BASE COUNT         1058 a         1920 c         1911 g          989 t
ORIGIN      
        1 aaggaccaaa gcagcccgta ccagaaggtg cggatctgcc agcggaagtg cgactcgagc
       61 caggtgcctt caacgtccct gcgtttcacg taattgacga tcaccgcggc gatgaaggtg
      121 atcccgacga ggaacgccgc gatctgcagg ccgtagacga tcgcggtgat ccgcttgagc
      181 gactgctcac cgggagcagc gctcgcgacg ggtgatgacg ttggggtcgg gtgcgcccgt
      241 gcagcggctg cccgggcttc ctcccgcctg agaaacgccc agccggcaac cgccagtgcc
      301 gcgccgaagc cgacaaacac gagcgcgatt gcgccggaca ccgccccgac gatgtggaac
      361 acgaggttcc gtcccaccga gaatacgcgc tgcgggaagt cccccgccac gacgatctca
      421 tagaggccgg gttgcgtgat gctgaattgc acgaccgcga tgctctcggt gctgccggtc
      481 gtggaagtcg cccccgaata cgagccgacc gagagcgcct gcccgctcga cttgtcgatc
      541 accgagatct gcgccccatg cggaaggtgc ctcggctcgt cgtagctgcg ccccttgaac
      601 accgtacgaa agtcgttcca caccagatag ctccccggct tcttgagctc cacggtgtgc
      661 cggccgggcg cgagaaacgg aacggctgct tcatattcca ggaacagcca cacggcaatg
      721 ccgccgaccg cgacgagggt ggcaaggacg atcgcagccg cgccccagta ccaccccatg
      781 ccggggatgc gtgtcgtatc agccatggcg ccaatccggc gcgtggcggg atcagcgcgt
      841 gccggcgcgc ttcagcagtt cgatgacctt ggcgtccctg ccctgtgccg cgtacgcgag
      901 caccgagccg cccggcccgc tccggttggg gtcagctccg tgcttgagca ggagttgcac
      961 tatcgaggca tcgccgttca tggctgccgc catgagcggg gtgacgccgt tcgaatccgg
     1021 gcggtccacc caccagccca gctcgagcag ctccgccgcg cccgcacggt cgcgatacat
     1081 caccgccgtc atgacgtcgt tgtacttcgg cgtcctcgcc ggcgccatgg gcggcgccgc
     1141 ggccgtttcc gcgggggccg ccggtttggg cttcgcgacg cggcgcttgg ccttgggttt
     1201 ctcgtcagcc gaaggcgcgg cctccggcgc cggcttgggt gccgcttccg ctttcttcac
     1261 cggtgcggcc gcgggtttaa caggcgtcgc ctgcgctgcc atcggcgcgg cggccggttt
     1321 cgcgggcgcg gttgcagccg gcttgacagc gggggttgct tccttcggag caggcgccgg
     1381 agtcggcgcg gtagccggcg gcgcgctcgg agtgacggcg gcgatctgcg tggcaggccc
     1441 agctgccggt ggcgccgata ctgcaggtgc agccctggcg accggcggct tggtcgcggc
     1501 cgccggcgct ttcgctaccg gcggtcttgc aggcacagcc ttcatgcccg gttgcccttt
     1561 catcgccccc ttcattgcct tttccatctc ccgccccatg tccttcgtca actgcggcag
     1621 atagacttcc gtgacgtagt aataggaggc gccggccgcg atcaccaacc cgccgaccgc
     1681 ggcgacgacc gccttgagga agctcgacgg cccggcgcgc acgacgaggc agctggtgat
     1741 catgtcgtgc agcgcctgct tgcgcgaagt gaaagcggcg agcaggaatc cgatacccaa
     1801 tggtatcgac gagatgatct tcgcgaggtt cctgagcagt gccctgatga atgaggtgcg
     1861 gtttccgtcg gcgtcggtga cctggatgcc gagcagctgt tttccgaacg tcgcctggcg
     1921 cgccgagctt tccagcaccg gccagtagag cagctggacg aagaagaaaa tgacgtagcc
     1981 gatcgccagg cccgtgccgc ccatggccat gcccagcacg atgaacagaa tgatcccgaa
     2041 gatcatcacg atcgccgagt ccaccagcag cgccgcgacc cgcgcccaga agccggcgta
     2101 aatcagcact ccaccacccg cggaagccgc cgcatcggcg ggcgtttcaa ccaccgcgtt
     2161 tcttttcgcg aacatggcta tcccccctcc agtggatgca cgacaacaat catactcctc
     2221 ctatgccggc agcattagcg tgcgcggggc gtcgcgggcc cggccccgca ttgacgccct
     2281 agcagcctgt cgaacttagc cgtctgacag gctgctaagg agtttggcgg accgaagttc
     2341 gacaaactcc tggggcatcc atagaatcca acatgccccg ttgcgcgttt gcgcgctggc
     2401 atcaccccgc taccaggagg accccatgcc caagacaccg ctcggcaggt tgccgcgcga
     2461 ggcgatccgg atgctggaat tgccgccgct gcagaaagaa atcctcgacg gcttcaaagc
     2521 gctcgttgat ctcaccggga cggtgtccga cgcgatggac gagctcggca tcgtcggcgt
     2581 cgtccccgct tacgtgctcc cgccggtcac cacgggaaaa agcatcgtgg ggccggcgct
     2641 caccgtgcgc aacatccagc gcaccgcgca gatccacaag gcagcgctgg acaagaccaa
     2701 cacgcagggc gagagcgagg cgcacaacct cgccgagtcg ggcgacgtgc tggtgatcga
     2761 gggcgtgacg ggttgctcca acatgggcgg gcagtcggcg accatcgccc ggcgccaagg
     2821 tttcatcggc gccgtggtgg acggcacggt gcgcgatccg cagcagtacc gcgagatggg
     2881 ctggcctgtg tggtgccgcg gtttcacgcc ggtcaccggc aagtggcgca tgcagactgt
     2941 ggagatcaac ggcacggtac aaattgccgg cgtgcaggtg aaaccgggcg atctcgtgtg
     3001 cgcggatgaa gcgggcgtcg ccttcgtgcc gcgcgagcag gccgcggcgg tgctggaagc
     3061 ggcgcgcaag atcgatacgg gcgacaccag gcgcaaaaag gacatcgaca gcggggcgag
     3121 cgtcgcggat ctgatcacca ggaaatacaa gtgacgcgtg atgagtgatg ggtgatgagt
     3181 gatataaagc cctcgaacct gttgttcatc ctgtccgacg agcacaacaa acgcgtgctc
     3241 gggtgttacg ggcatccgat gatccagact ccgaacctcg atgcgctcgc ggcgcgcggg
     3301 acaagattca gcaacgctta caccaactgc ccgatctgcg tgccggcgcg cgcgagcttc
     3361 gcgaccggtc gctacgtgca cgacgtgcgc tgctgggaca atgcgatcgc ctacaccggg
     3421 caggcgccat cctggggcca tcggttgatg gagcagagcc atcgcgtggt ctcgatcggc
     3481 aagttgcact acaaggaatc gaagccggaa gtgaacgggt tcgacgagga gatcctgccg
     3541 ctgcacatcg tcaacggcat gggcgacctg ctgggcctga tccgcgacga gctgccacgg
     3601 cgcccaggct cgctcaagct gggccctgaa gccggtcccg gcgagtcgga gtacacccgc
     3661 tacgaccgct cgatcgccga tgaaaccgtg aagtggctga acagcgaggc ggcgagacgc
     3721 cgcgacaagc cgtgggcact ttacgtcggc ttcgtctccc cgcatttccc gctgatcgcg
     3781 ccgccgcggt tctacgatct ttatccggaa aacaaagtgc cgtggcccga cctttacgat
     3841 ccagccgaac ggccgcgtca cccgtttctc gacgccatgc gcaagtgcct gtgcttcgac
     3901 gagcctttcg atccgccgat ggtgcggcgg gcgatcgcgg cctacttcgg cctcgtctcg
     3961 ttcctcgatg acaacatcgg aaaaatcctg cgcgcgctgg aggagaccgg gctcgcgaag
     4021 agcacccgcg tcgtctactc ctccgaccac ggcgacaatc tcggcacgcg cggcctgtgg
     4081 ggaaaatcca cgatgtacga ggagtcggcg ggcatccctt tgatcatggc gggcccggac
     4141 gtgccggccg gcggcacatg cgataccccg gtgacactgg cggactgttt cccgacgttc
     4201 atccacgcgc tcggcgcgaa gcccgatccg cgcgacaggg atctgccggg cgtctcgctg
     4261 ctcggcatcg cgcggggcgc ggtcccgcag cggaccattt tctccgaata ccacgccgcc
     4321 ggcgccatca ccgccagcta tatgatccgc cacggccggt acaagtacat ccactacgtg
     4381 gggatgccgc cgatgctgtt cgacctggaa gcggacccgc atgagcgcag agacctcgca
     4441 tgcgagtccg cttacgagaa aatcattgca gaatgcgaag ggctcctgag aaaaattgtg
     4501 gatcccgaag cagtggacaa gctcgcgcgg gccgaccaga aggcgcacat tgagaagaac
     4561 ggcggggagg aagcgatcct gaagaaaggc acgttccgct actcgccgcc gccgggggcg
     4621 gaagccacgt attactacta ggaaaagcgt gataagtgac gggtgataag tgataagaca
     4681 ccccgcgctc gcgcgggaga taattcctat cacctatcac ccatcaccta tcacctatca
     4741 cctatcacgc attattcagc tttgatacca gtagcccgaa tgaccttgcc ccagcgcgcg
     4801 tagtcctcgc ggatgagccg cgccagctct tgcggcgagc cggtctttac atcgatacct
     4861 tgcgaagcga gccgcgcctt gacgtcgggc gtgttgagca ccctggcaat gtccgcgttt
     4921 gccttggcga tgatcgcggg cggaactttc gccggcatca tcacgccgga ccagacgtcg
     4981 tggtgatagc cgggaacgcc cgactcggcg atcgtgggca cgtccggcag cagcggcagc
     5041 cgctgcgcgc cggcggaggc gagcagccgg agccgccctt ccttaatctg cgggagcagc
     5101 tggttggccg ccccgatgaa aacgtgtatg cgtccgggca gcaggtccac cagcgccggc
     5161 cctgctcctt tgtacggcac gtgggtcatc tcgatgccgg cgaggtattg cagcagcgcg
     5221 gtgccgaggt gctgcgcgct gccgttgccg gaggagccgt agttgagcga tcccggtttc
     5281 gatttggcga gcgccaccag ttcctgcacc gatcgcgccg gcaccgaggg gtgaaccacg
     5341 agcaggaact ggaagcgcgc ggtctacgtc acgggcgtga agtccttgag cgggtggtac
     5401 ggcgtcttgt cgagctgggg gatcacgacc atgatgttgt cggtcgtgag gaaccatgtg
     5461 ttgccatcag gcgcgctctt cgcgaccagg tccgcggcga ggatgcggcc tccggccgta
     5521 cggttttcca ccacgaccgg ctgcggccag ttgatctccg ttgcaatggt gcgcgctatg
     5581 gtatcgggcg agccgcccgg gggcagcggc acgatgaacc tcacgggacg cgatgggtag
     5641 ctctccactc ccttcgaagg accctgggcg aaggcgccgc acgttgctgc agcaagcacg
     5701 gcggtagcga gcagtcggaa cggcatgcgt cccctccttt gatcggtttt tacgatcaat
     5761 gcgtgtagtt taatttgatt gctctgttac tggccatccc ctacattgtc cctgccaacg
     5821 cgcatacagg agaacatcat ggccatcgac atcgggatca agccgcagga ccgcaagg
//
LOCUS       DBNGWZ010000040         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1263014, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000040 DBNGWZ010000000
VERSION     DBNGWZ010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1263014"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="AC16CX_01165"
     CDS             complement(<1..764)
                     /locus_tag="AC16CX_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3011151.1"
                     /translation="MYAALRNRFHNWLFQPRGRESGAIVLVQRRVFILPTRQGLMFAV
                     VLLVMLTGSINYSLGLGFVLTFMLGALGVNAMIYTWRNLANLRVTGGRAHPVFAGDAA
                     QFTVHLENTSDTGRHAIGLTYGGKQVTFVDVVARATTQATVGILAPRRGILRPGRMTL
                     FTRFPLGLYYAWAYLELDMHCLVYPRPAFPGLPLPPALASAGAGAERGRGQEDFSGLR
                     QYHVGDSPRHIAWKAAARDQVLLTKLFSGRAETELWL"
     gene            complement(838..1755)
                     /locus_tag="AC16CX_01170"
     CDS             complement(838..1755)
                     /locus_tag="AC16CX_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011152.1"
                     /translation="MNQRIEIVVAQVGGIILGKEHQIRLAITCLIARGHLLIEDIPGV
                     GKTMLAHALAASLGLGFQRIQFTSDLLPADIIGVSIYNRDTSGFRFHAGPIFTQVVLA
                     DEVNRATPKAQSALLEAMEEHQVTTEGETRHLPEPFFVIATQNPSYQVGTFPLPESQL
                     DRFLMRIHLGYPDSEAERALLQGEDRRDLLAALKPAMMTQELTELQHRARQIHTSSAL
                     LDYVQALADHTRRVPDYVNGLSPRAALALLHAARAWALMAGRGHVVPEDVQTVLPAVV
                     GHRLVPAGEVTRADGADVADHLIKQVAIP"
     gene            complement(1870..>2385)
                     /locus_tag="AC16CX_01175"
     CDS             complement(1870..>2385)
                     /locus_tag="AC16CX_01175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3011153.1"
                     /translation="PSHLVQRLTLKDGTAVTLRPIRPEDARIEQEFVRNLSDESRYYR
                     FMDSVRELSPRMLTHFTRLDYDRHMALIAVTERGGREAQVAVARYVADDDRRRCEFAI
                     VVADDWQHKGLGTRLMQALMGAARAAGIHTMFGEVLASNHKMLQFTAGLGFSAHFDER
                     DPRMMRVETVL"
BASE COUNT          421 a          754 c          805 g          405 t
ORIGIN      
        1 agccacagct cggtttcggc gcgcccgctg aacagcttgg tgagcaggac ctggtcgcgt
       61 gcggccgcct tccacgcgat gtgacggggg gaatccccca cgtggtactg gcgcaggccg
      121 gaaaaatcct cctgtccccg gccacgctcg gcgccggcgc cggcgctcgc gagcgccggc
      181 ggcagcggca ggccagggaa cgccggccgc gggtagacga gacagtgcat atcgagttcg
      241 agataagccc aggcgtaata aagaccgagc gggaaacggg tgaacaacgt catccggccc
      301 ggccggagaa tgccgcgtcg cggagccagg attccgaccg tcgcctgcgt tgtggcgcgc
      361 gccaccacgt ccacaaacgt cacttgcttt ccgccgtagg tcagaccgat ggcatggcgg
      421 ccggtgtcgc tggtgttctc cagatgcacc gtgaattgag cggcatcgcc ggcgaacacc
      481 ggatgcgcgc gccctcccgt cacccgcagg ttcgccagat tgcgccaggt gtagatcatg
      541 gcgttcacgc ccagcgcccc cagcatgaaa gtgagaacga agccgaggcc cagcgagtaa
      601 ttgatcgaac cggtgagcat caccagcagc accacggcga acatcaggcc ctggcgcgtg
      661 ggcaggatga acacccgccg ctgcaccagc acgatcgcac cgctttcccg gccgcgcggc
      721 tggaacagcc agttgtggaa acggttcctg agggcggcgt acatgcgagt gattgagtga
      781 ctaagtaact aagtgattga gaatccggcc gccggaggtt tctcagtcac ttaattacta
      841 gggtatggcc acctgcttga taaggtggtc cgcgacatcg gcgccgtcgg cccgggtgac
      901 ctcaccggcg gggaccagcc ggtggccgac gaccgccggc agcacggtct gcacgtcctc
      961 gggcacgacg tggccgcgcc ccgccatcaa ggcccaggcg cgggcggcgt gcagcagcgc
     1021 cagcgccgcc cgcggtgaca acccgttgac gtagtccggg acgcggcggg tgtggtccgc
     1081 cagcgcctgc acgtagtcga ggagggcgct ggaagtgtgg atctgccgcg cgcgatgctg
     1141 caattcggtc agttcctgcg tcatcatcgc gggtttcagc gccgcgagca ggtcgcgcct
     1201 gtcctcgccc tgcagcaacg cccgctccgc ctcgctgtcc ggataaccca gatggatgcg
     1261 catcagaaaa cggtcgagct gcgattccgg cagcgggaac gtgcccacct gatacgaggg
     1321 gttctgcgtg gcgatcacga agaatggctc gggcagatgc cgcgtctcgc cttcggtcgt
     1381 cacctgatgc tcctccatgg cttcgagcag cgcgctctgg gccttgggcg tcgcgcggtt
     1441 gacttcgtcg gccagcacca cctgggtaaa gatcgggccc gcgtggaacc ggaagccgct
     1501 ggtgtcccgg ttgtagatgg agacgccgat gatgtcggcg ggaagcagat cgctggtgaa
     1561 ctggatgcgc tggaatccca ggccgagcga ggccgccagc gcgtgcgcga gcatggtctt
     1621 gcccacgccc ggaatgtcct cgatcaacag gtggccgcgg gcgatcaggc aggtgatcgc
     1681 caggcggatc tggtgctcct tgccgaggat gatgccgccg acctgggcga cgactatctc
     1741 gattctctga ttcatgctgc gcggaacgtg accatgcgtt gagtttgtca cattataagg
     1801 tgccggtggc ccggaaaagc gtgatgggtg ataggtgata ggtggtaggt gaatcacgat
     1861 tcacaccttt caaagcactg tttccactcg catcatgcgc gggtcccgct cgtcgaaatg
     1921 cgcgctgaag ccaagcccgg ccgtgaattg gagcatcttg tggttgctgg ccagcacttc
     1981 gccgaacatg gtatggatgc cggcggcgcg agcagctccc atcaatgcct gcatcagccg
     2041 ggtcccgagg cccttgtgct gccagtcgtc ggcgaccacg atcgcgaatt cgcagcgcct
     2101 gcggtcatcg tccgcgacat agcgcgcgac ggcgacctgt gcctccctgc cgccccgttc
     2161 ggtcacggca atgagcgcca tgtggcggtc gtagtccaac ctcgtgaagt gcgtaagcat
     2221 ccgcggcgac agctcgcgca cggagtccat gaagcggtaa taacgcgatt catccgagag
     2281 gttgcgcacg aactcctgtt cgatccgggc gtcctccggg cggatcgggc gcagcgtgac
     2341 cgcggtgccg tccttcagcg tcagccgttg gacaaggtgg gaggg
//
LOCUS       DBNGWZ010000041         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3152425, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000041 DBNGWZ010000000
VERSION     DBNGWZ010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3152425"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="AC16CX_01180"
     CDS             complement(<1..841)
                     /locus_tag="AC16CX_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011154.1"
                     /translation="MILHAATIFLSAFLLFLVQPVIAKQILPWFGGAAAVWATCLVFF
                     QSVLLFGYAYSDWTTRLLAPRRQAWLHVALLAASLLMLPIIPDARWKPGGESGEPSLL
                     ILGLLGATIGLPYFLLSTTSPLVQAWFWQRFRHAVPYRLFALSNFASLLALLSYPVLV
                     EPFLPLAAQSWLWSVAYGSFVLLCGATAYISTRGPAPAAAPVEQTEIRSEQAPTIGDR
                     LLWIALSAMGSCLLLAVTNHLTQNIASIPFLWVVPLSLYLITFILCFDHPRWYHRGVF
                     LALA"
     gene            1098..>2488
                     /locus_tag="AC16CX_01185"
     CDS             1098..>2488
                     /locus_tag="AC16CX_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3011155.1"
                     /translation="MEEALATNERKAALGTTVLKSVCRSCHGGCGTLLHVSEGRLTKV
                     AGDPNSPLNRGRLCPIGAATLDLVYHPDRLHHPLRRAAGRGEGQWKRISWDEALGEIA
                     GRLLAIKRKFGAEAIALGTGTGRHHIRWVSRFGHALGTPNWCEPGFAQCFHPRVNTCL
                     LTFGDFPVCDFTGGTPPRCIVYWGHNPVNSGPDGETRFNAIDSLSSRPRIIVVDPRRT
                     RLAKKADLWLQLRPGTDDALALAMLNVLVSEKLYDAPFVTRWTYGFDALAERVRGCTP
                     EWAEPITWVPAEKIRAAARLYAQTRPSMMEWGCAIEHTPNCIQTVRAVSMLPALTGNV
                     DVPGGWVFGMHGIGRFPSLIENLSPEMNAKRLGADRFRMLCGEGADLPAAHIPSLLRA
                     MRDGVPYPVKAFLVFGNNTLTTYGNARQAYESLLKLDLLVVADLFMTPTAELADIVLP
                     AAAWPELNQIAGLP"
BASE COUNT          482 a          784 c          833 g          389 t
ORIGIN      
        1 ccgccagcgc gaggaacacc ccacggtgat accagcgcgg atggtcgaag cacaggatga
       61 aggtgatgag atagagcgaa agcggcacca cccacaggaa cgggatcgaa gcgatgtttt
      121 gcgtgaggtg gttggtgacc gcgagcagca ggcacgaacc cattgccgag agcgctatcc
      181 acagtagacg gtcgccgatc gtaggcgcct gctctgaacg gatttcggtt tgctcaaccg
      241 gcgcagcagc gggggccggc ccgcgggtgc tgatatacgc ggtcgccccg cacaggagca
      301 cgaagctgcc ataagcgacg gaccagagcc acgactgcgc ggcgagcggc aggaacggct
      361 cgaccagcac cgggtacgac agaagcgcca gcaacgacgc aaagttggac agcgcgaaca
      421 ggcggtacgg caccgcgtgc cgaaagcgct gccagaacca ggcctgcacc agcgggctcg
      481 tggtggacag caggaagtac ggcaggccga tagtcgctcc gaggagcccc agtatgagca
      541 ggctgggctc gccgctttcc ccgccgggct tccaccgcgc gtccggaatg atcggcagca
      601 tgagcaggct cgcggcgagc aacgcgacgt gcagccaggc ctgccggcgc ggggcgagca
      661 ggcgcgtcgt ccaatcggaa taggcgtagc cgaacagcag caccgactgg aagaacacca
      721 ggcaggtggc ccataccgcg gccgcgccgc cgaaccacgg caggatctgc ttggcgatga
      781 cgggctggac caggaacagc aggaaggcgc tgaggaagat cgtggcggcg tgcaggatca
      841 tttggccatt aacggataaa aagcgcgcta gtgtaccgca tcaggtaccc aaaaggatga
      901 aggatgaagg cagaaggatg acctggctct agtcccgcac tgcgggggtc tgccttctga
      961 cttctgcctt gttcaccggt ggcgccgccc aatccgctgt cagtttgatg ttgatcaaat
     1021 gggtggcgca ggtgccgtgg caaattgggg cgaatggggt cggtcaggtc ccatcaaaac
     1081 tcgaaggaag taacgttatg gaagaggcgc tggcaacgaa cgaacggaag gcggccttgg
     1141 gcacgaccgt gctgaagagc gtctgccgca gctgccacgg cggatgcggc acgttgctcc
     1201 acgtcagcga gggccggctg acgaaggtcg cgggcgatcc caattcgccc ctcaatcgcg
     1261 gcaggctgtg cccgatcggc gcggcgacac ttgaccttgt ctatcacccc gaccggctcc
     1321 accacccgtt gcgtcgggcg gccggacgcg gcgagggcca atggaaacgg atctcgtggg
     1381 acgaggctct gggcgagatc gccgggcgcc ttctggcgat caagcgcaag ttcggcgccg
     1441 aggcgatcgc gctgggcacc ggaaccggcc ggcaccatat acgctgggtg tcccgcttcg
     1501 ggcacgcgtt ggggacgccc aactggtgcg aacccggctt cgcgcagtgt ttccatcccc
     1561 gcgtcaatac ctgcctgctc acctttggcg attttcccgt gtgcgacttc accggcggca
     1621 cgccgccccg atgcatcgtg tactggggac acaatcccgt gaactcgggt cccgacgggg
     1681 agacgcgctt caacgccatt gactcgcttt cgagccggcc gagaatcatc gtggtcgatc
     1741 cgcgccgcac acggctggca aaaaaagcgg atctttggct gcaattgaga cccggcaccg
     1801 acgatgcgct tgcgctcgcg atgctgaacg tgctcgtttc ggagaagctg tacgacgcgc
     1861 cttttgtcac ccggtggacc tacggcttcg atgcgctcgc cgaacgcgtg cgaggctgca
     1921 ccccggaatg ggccgagccc attacctggg tgcctgcgga gaagatccgg gcggcggcgc
     1981 ggctatacgc gcaaaccagg ccgtcaatga tggaatgggg ctgtgcgatc gaacacacgc
     2041 ccaactgcat ccagaccgtg cgcgcggtat ccatgctgcc ggcgctcacc ggcaacgtcg
     2101 acgtgccggg aggctgggta ttcggcatgc acggcatcgg ccgattcccc agcctgatcg
     2161 agaatctgtc cccggagatg aacgcgaagc gcctgggcgc ggaccggttc aggatgctgt
     2221 gcggcgaagg ggcggatctt cccgctgcgc atattccgag cctgcttcgt gccatgcgtg
     2281 acggcgtgcc ctatccggtc aaggcctttc tcgtgttcgg caacaacacg ctcaccacct
     2341 acggcaacgc cagacaggcc tatgagtcgt tgctcaagct cgacctgctg gtcgtcgccg
     2401 atctgttcat gacgccgacc gcggagctcg ccgatatcgt cctgcccgcc gccgcgtggc
     2461 ccgagctcaa ccagatcgcc ggcctgcc
//
LOCUS       DBNGWZ010000042         2568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_129877, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000042 DBNGWZ010000000
VERSION     DBNGWZ010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_129877"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC16CX_01190"
     CDS             <1..572
                     /locus_tag="AC16CX_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3011156.1"
                     /translation="PPRNPLRWPIYASVAAAIVGVLAVYEFFWNRPETTIAQPGVTTA
                     PPAAPQPNTMPPHPMTALTSSSSSAESHEKAVQPAISAEAAATTTQDAPPPAAAEAQQ
                     DPVKPPKAGERQVRLVFSEESWVEIRDRNEKVIFSQLNRPGTRRLINGLPPFSIVVGN
                     AHGVRLTYEDKAVDLARHTKIDVARLILQ"
     gene            569..1852
                     /gene="ispG"
                     /locus_tag="AC16CX_01195"
                     /gene_synonym="gcpE"
     CDS             569..1852
                     /gene="ispG"
                     /locus_tag="AC16CX_01195"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3011157.1"
                     /translation="MKCYPEHGPAPRRGSRQVWVGHVAIGGDAPIVVQSMTNTDTADV
                     PATVQQVAELARAGSEIVRVTVNNAEAAAAVPVIRERLDAMGVNVPLVGDFHFNGHRL
                     LTQHPACAEALAKLRINPGNVGRGSKRDEQFATMIEFACRYGRPVRIGVNWGSLDPEL
                     LARMMDENARQPEPEDAMEVTRRALVASALESAQQAESLGLAHDRIVLSCKVSGVQDL
                     VSVYRDLAASCDYALHLGLTEAGMGSKGIVASTAALAMLLQEGIGDTIRISLTPEPGG
                     DRTREVVVAQEILQTMGLRSFAPMVIACPGCGRTTSTYFQELADRIQSWLRKQMPLWR
                     KRHPGVENMHVAVMGCVVNGPGESKHANIGISLPGSGENPVAPVYVDGVKTVTLKGGN
                     IAAEFQKIVEQYVVDTYSKSTAAGEKKPAKDPAHA"
     gene            1996..>2568
                     /locus_tag="AC16CX_01200"
     CDS             1996..>2568
                     /locus_tag="AC16CX_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3011158.1"
                     /translation="MNDVLPDEAQLWEFFEDTVRGWLRAYGYRNIRTPILEKTDLFVR
                     SIGAETDIVEKEMYTFTDGLSRENLALRPEGTASCVRAVIEHNLLYGGPQRLWYWGPM
                     FRHERPQKGRYRQFHQVGVEAFGFGGADIDAEHLVMCARLWRSLGLEGIALDLNTLGN
                     SETRSRYRKRLVKYLERHESRLDADSRRRLH"
BASE COUNT          494 a          835 c          848 g          391 t
ORIGIN      
        1 tcccgcccag gaacccgctg cgctggccga tctatgcgtc cgtcgccgcg gccatcgtgg
       61 gcgtcctcgc ggtttacgag tttttctgga acagacctga aaccacgatc gcgcagccgg
      121 gcgtaacgac cgcgccaccc gcggcgccgc agcccaacac catgccccct catcccatga
      181 cggcgctcac ctcgtcctct tcctcagcgg agagtcacga gaaagcggtc caaccggcca
      241 ttagtgcgga ggcggccgca acaaccacac aggatgcgcc acccccggcc gccgccgaag
      301 cgcagcagga cccggtcaag ccgcccaagg caggcgagcg gcaggtcagg cttgtcttca
      361 gcgaggaatc atgggtggaa atccgcgacc gcaacgagaa agtcatcttc tcgcaactga
      421 accgaccggg cacgcggcgg ctcatcaacg gcctgccgcc gttttcgatc gtggtcggca
      481 acgcgcacgg cgtgcggctc acctatgaag acaaagcggt cgacctggca cgccatacca
      541 agatcgatgt cgcgcgcctg atactgcaat gaagtgctat cccgaacacg gaccggcgcc
      601 gcgccgcggc agccgccagg tatgggtcgg ccatgtcgcg atcggcggcg atgccccgat
      661 cgtggtccag tccatgacca ataccgatac cgcggacgtc cccgcaaccg tgcagcaggt
      721 ggccgagctg gcgcgcgccg gctccgagat cgtgcgcgtg acggtcaaca atgccgaggc
      781 ggcggcggca gtacccgtga ttcgcgagcg gctcgacgcg atgggcgtca acgtgccgct
      841 ggtgggcgat tttcatttca acgggcaccg cctgctcacc caacacccgg cgtgcgccga
      901 ggcgctggcg aagctccgca tcaatccggg caacgtcggc cgcggctcga aacgcgacga
      961 gcagttcgcc acgatgatcg agttcgcctg ccgctacgga agaccggtgc gcatcggcgt
     1021 caactggggc agcctcgatc cggaactcct cgcgcgcatg atggatgaga acgcccggca
     1081 accggaaccc gaggatgcca tggaggtgac ccgcagggcg ctggtcgcct ccgccctgga
     1141 gagcgcgcag caggcggaaa gcctcggcct ggcgcacgat cgcatcgtgc tctcgtgcaa
     1201 ggtgagcggc gtccaggatc tggtctcggt ctaccgcgat ctcgcggcga gctgcgatta
     1261 cgcgctgcac ctgggcctga ccgaggccgg catgggctcg aagggcatcg tcgcttccac
     1321 ggcggccctg gcgatgctgc tgcaggaggg catcggcgac accatccgca tttcgctcac
     1381 gcccgagccg gggggagacc gcacccggga ggtggtggtg gcgcaggaga tcctgcagac
     1441 gatgggattg cgctcgtttg ccccgatggt gattgcctgc cccggatgcg ggcgcaccac
     1501 cagcacctat ttccaggaac tcgccgaccg catccagagc tggttgcgca agcagatgcc
     1561 gctctggcgc aagcgccatc ccggcgtcga gaacatgcac gtcgcagtga tgggctgcgt
     1621 ggtgaacggg ccaggggaga gcaagcacgc caacatcggg atcagcctgc ccgggtcggg
     1681 cgagaacccg gtcgcgccgg tttacgtgga cggcgtcaag accgtaacgc tcaagggcgg
     1741 gaacatcgcc gcggaattcc agaagatcgt cgagcaatac gtggtcgaca cctattcgaa
     1801 aagcaccgcg gcgggcgaaa agaagccggc gaaggatcct gcacacgcct gaagcaggtg
     1861 actgagtgac taagcgactg agtgattgag ctatcagtca ttcaggacgc ggatgaatgc
     1921 ggcttggctc agtcacccag tccctaatca cttaatcact cgatgaaatc agagacgata
     1981 caggccatcc gggggatgaa cgatgtcctg ccggacgagg cgcagctgtg ggaatttttc
     2041 gaggacacgg tgcggggctg gctgcgcgcc tacggttacc gcaacatccg cacccccatc
     2101 ctcgagaaga cggatttgtt cgtgcgctcg atcggcgcgg aaaccgacat cgtcgagaag
     2161 gagatgtata ccttcaccga cggactgagc cgcgagaacc tcgccctgcg gccggaaggc
     2221 accgcgtcct gcgtgcgggc ggtgatcgag cacaacctgc tttatggagg gccgcagcgg
     2281 ctgtggtact gggggccgat gttccgccac gagcggccac agaaaggccg ttatcgccag
     2341 ttccaccagg tcggcgtgga agcgttcggt tttggcgggg ccgacatcga cgcggagcac
     2401 ctcgtcatgt gcgcccggct gtggcgcagc ctcgggctcg aaggcatcgc gctcgacctc
     2461 aacacgctgg gcaacagcga gacgcgttca cgctaccgca aacgcctggt gaagtacctg
     2521 gaacggcatg agagccggct cgatgcggac tccaggcggc ggttgcat
//
LOCUS       DBNGWZ010000043        38300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2648557, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000043 DBNGWZ010000000
VERSION     DBNGWZ010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 38300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 38300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38300
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2648557"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="AC16CX_01205"
     CDS             complement(<1..789)
                     /locus_tag="AC16CX_01205"
                     /inference="COORDINATES: protein motif:HMM:NF045728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F510_1955 family glycosylhydrolase"
                     /protein_id="HIR3011159.1"
                     /translation="MKAVLERTRKLAAPLVTAAAFAFLTTAEGGAQPVVTLHHVHGLA
                     FSHDGKRLMVPSHHGLAVYSAGKWSKASGQAHDYMGFAATRTRLYSSGHPAQGSALID
                     PFGLIRSADGGKNWEKLSLEGESDFHLMAASWNTNAVYVWNSERNSRMQGPGLYYTVN
                     DGFMWQRAAAKGLAGKPRALAVHPSDAKTIAAATSSGIYVSRNSGDSFEPLARGAEGL
                     AVSFDFDGKHLWHSTYDSRPRLASVELQGGAAAAVALPSLPNDAV"
     gene            complement(825..1169)
                     /locus_tag="AC16CX_01210"
     CDS             complement(825..1169)
                     /locus_tag="AC16CX_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012753.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3011160.1"
                     /translation="MDIKLIVAMIRRDRLEQVEKTLQDIGVERINVSKVKGYGEYHDF
                     FERSWMVEEVRIEIYTRTDEVEAITAAIMNAAHTGLPGDGIVAVMPVEKLFLIRTRSE
                     ATPEEFWPQIES"
     gene            complement(1194..4346)
                     /locus_tag="AC16CX_01215"
     CDS             complement(1194..4346)
                     /locus_tag="AC16CX_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012211075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3011161.1"
                     /translation="MLNRIIEWSARNVFLVLLGTAFIVGAGIYAVAKTPLDAIPDLSD
                     VQVIIYTEYPGQAPQVVEDQVTYPLTTAMLSVPRSKVVRGFSFFGASFVYVIFEDGTD
                     IYWARSRVLEYLNFASGRLPRGITPSLGPDATGVGWVYQYAVIGAKRSLAELRTLQDW
                     FIRYQLTKAQGVAEVASIGGFVQQYQVIVDPRKLQAYGVPLSRIAQVIRMSNTDVGGR
                     VVEMAETEFMVRGRGYLRGVSDLEQLVVKSEGGIPVMLRDVARIELGPDERRGITELN
                     GEGEVVAGIAMARYGQNALEVISNLKSKIEEIASGLPPGVAIEPVYDRSDLIHRAIDN
                     LKRTLIEESLIVALVCIVFLLHVRSALVAIIMLPIGVLIAFIAMRFLGLNSNIMSLGG
                     IAIAIGAMVDAAIVMIENAHKHLERLEPGASRLEAIITACREVGPALFFSLLIITISF
                     LPVFTLEAQEGRLFAPLAYTKTFAMAGAALLSITLVPVLMLLFIRGRIRSESANPLSR
                     IMIAVYRPVLSTALRHRAWVLVVAGLVLSMTLYPATKLGTEFMPTLNEGTLMYMPTSL
                     PGMSVTKAAEVMQIQNRIIKSFPEVASVFGKAGRANSATDPAPIEMFETVINLKPQSE
                     WRSGMTVERLIAELDRAVQMPGVSNAWTMPIKARIDMLSTGIRTPIGIKVFGKDLGEM
                     ERLAKQIEAVVKTVPGTTSAYAERITGGYFLNVEPDRMALARYGLAVGDLQEVVAIAL
                     GGEMVTTTVEGRERFGVIVRYPRELRNDPRMIATQVLVPVMNGAMVPLGQVAQVTLTQ
                     GPPGIRTENALLSAYIFVDIRNRDIGGYVADAQKAVREQVKFPPGYYATWSGQFEYLE
                     RAKERMKVVVPVTLLLIFVLLYLNFNRLTETLIVMASVPFSLVGGIWLMYLLDYNMSV
                     AVAVGFIALAGVAAETGVVMLIYLDQAFSAASARRHAARELVTIADLDDAIMRGAVLR
                     VRPLMMTVFAIIAGLLPIMWGTGTGSEVMRRIAAPMVGGMISATILTLIVIPAIYALV
                     KGAMITRSGTVAAPKSRTAS"
     gene            complement(4348..5928)
                     /locus_tag="AC16CX_01220"
     CDS             complement(4348..5928)
                     /locus_tag="AC16CX_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3011162.1"
                     /translation="MNRIANSILVVVLIATALAAGYWWGSQKAEGGRWKVEGASVTPT
                     AEKAESKRKILYYRNPMGLPDTSPVPKKDQMGMDYIPVYEGEEPESTGPVVKVSIEKV
                     QKLGVKTEAAAARELVRTVRAVGTVQIDERQLYAVAPKFEGWIERLHVNTTGQPVARG
                     QALMEVYSPDLVTAQQEYLIAWKGVESVKDGTPEIQASMRQLVASALTRLRNWDISEE
                     ELKQLQKEGRARQTLMLRSPVAGVVLEKPALKGMRFMPGEVLYKISNLSSLWLLADIF
                     EQDLAHVQPGQTARITVNAYPGKVFTGKVAFVYPTVTPETRTAKVRIELSNPGGLLKP
                     AMYASVELAAGRMKTKALAVPDSAVLDSGTRQIVLVQRGEGQFEPRPVKLGAHADGYV
                     EVLEGVQAGEMVVVSANFLIDSESNLKAALATFGAAAGPGGAPEATPGTVQVHKGQGT
                     VESIDYTGAAVTVSHGEIPSLKWPAMTMDFRVEDKALLTTVRPGQSVQFDIVQRGPGE
                     FVITRIAPGAAAAEHKRH"
     gene            complement(5968..7269)
                     /locus_tag="AC16CX_01225"
     CDS             complement(5968..7269)
                     /locus_tag="AC16CX_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3011163.1"
                     /translation="MRFTPTRAVPMQLRALVPVLMLLAAPQTIMDAAAQGTTPIQAVV
                     REALQNNPEIRAAARERDAAQQRVAPAGTLDDPMLEAGVLNVPTNSFRFDREDMTMKM
                     IGLSQRFPYPGKRGLRQDVASRDADSAAHGYQETVNRVARDARASYLDLALVIETTRL
                     TEKNKIILEQFLKITEARYAVGQGNQADVLKAQTQLSKMVDELIKLARERPMIEAELN
                     RALGRNTGTAAPLPEAPQLRPVSLALDTLRETAFRQRPQLLALQSIVARNERTLELAH
                     KEYFPDFDVRFSYGQRENMPDGTRRSDMVNFTVAINLPVWRESKTAPRVAEARAMHEQ
                     ALGMHEAQRNELAAKLRQQVASAEQNLRSARLYQGNILPQARLTVEASLAAYRVNRVD
                     FMTLLDNQITVFSYEVAHVAAVVNYNKALAEIDLLTGKSPL"
     gene            complement(7338..7727)
                     /locus_tag="AC16CX_01230"
     CDS             complement(7338..7727)
                     /locus_tag="AC16CX_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011164.1"
                     /translation="MVLSRLTRRAVAGVAAVLVLLCQSAALARACAPSADQPAAAMAQ
                     TPCHSAVGDDVSGTNQDAYQPGRCLSQAVSPPSAVPDLPPAAALPVFAVRAISFSTDD
                     AALPAVAPGLARIESPPLAILHCCLRN"
     gene            complement(7862..8734)
                     /locus_tag="AC16CX_01235"
     CDS             complement(7862..8734)
                     /locus_tag="AC16CX_01235"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3011165.1"
                     /translation="MIVRGTDRVIVMGITGKQGTFWTERMQAYGTRVVGGTNPNKAGT
                     MRCNVPVFATARDAMNGAGFEAAVLFIPPLMVKEAAMDAIEAGAKGLVVLTEHIPVHD
                     VMYFVAAARERGAFIIGPNTAGAVTPGECFAGFMPAFNPHIFRKGSIGVVSRSGSLGT
                     LLCLNVVQAGYGESAFIGIGGDPIIGTTTREALVALDNFPGTEAVVMVGEIGGSMEEE
                     AADYARTMTKPVVAFIAGGAAPQGRKMGHAGAIVMGDKGSYASKRSALEAAGVTVLDT
                     PSDVGAALKARLGR"
     gene            complement(8835..9959)
                     /gene="sucC"
                     /locus_tag="AC16CX_01240"
     CDS             complement(8835..9959)
                     /gene="sucC"
                     /locus_tag="AC16CX_01240"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3011166.1"
                     /translation="MNFEEHAAKPLLARHGIAVPRGGLAKTPDDAAEVAAGIGAVVVK
                     AQVPAGKRGKAGGIRLAATPDEARTHAQAIIGMEIAGHKVEKVLVEEQLPIERELYAA
                     VLNDPSTKGPLVMFSTEGGMDIEEVAATAPGKLRRQPVDIRRGFSHADARTLVRGLGL
                     GAAEGKVADALARLYQAYAAFDAELLEINPLVVTRDGRVVALDCKFVMDDCAIVRHPD
                     IAPGGTPEKLTALEARAKATGLKYIELDGDVGVLANGAGLTMTTMDMIRHHGGRPANF
                     LEIGGEAYTQAKPALELLLSKPGIKSLVINFCGAFARTDVMTGGVIEAIEALKPRLPI
                     FFSVHGTGDEEAIRMLKDRLGVTPLTTMDEASKAAVEAAA"
     gene            complement(10209..11639)
                     /gene="sauS"
                     /locus_tag="AC16CX_01245"
     CDS             complement(10209..11639)
                     /gene="sauS"
                     /locus_tag="AC16CX_01245"
                     /EC_number="1.2.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylating sulfoacetaldehyde dehydrogenase"
                     /protein_id="HIR3011167.1"
                     /translation="MASRGAAPGDSTAARNAVANLIGKARAAQKVFERSSQEQLDEAV
                     TAVGWAIMNPEHNRVLAELAVKDTGLGVVSDKVAKNHRKTLGLLRDLKGAKSTGVIAE
                     YPEKGITEIARPVGVVAAVVPSTNPAATPANNIINALKCGNAVIVSPSPKGYSTCTLL
                     LKYVHAELKRISAPVDLVQHLPSPVTRELTTELMKQADLVVVTGSQNNVRAGYSSGTP
                     ALGVGTGNVAVIVDDSADCADAAKKIMASKIFDNATSCSSENSVIIQGRVYDKMIKAL
                     TKEGGALLTQDEKAALQKAMFPGGMLSAAVTAQPVKKICAVAGLKRPDLVNAKCLLVE
                     ETGTGRSHPFSGEKLSPVFAVYRARDFDHAFDIVRAIYDYQGNGHSCGIYTENEAHIR
                     KLAHELTVARVIVKQAHTFATGGSFDNGMPFSLSMGCGTWGGNSFSDNLHYKHFMNIT
                     RIVRTIPPREPILDDIFGHYCKKHGL"
     gene            11834..13267
                     /locus_tag="AC16CX_01250"
     CDS             11834..13267
                     /locus_tag="AC16CX_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HIR3011168.1"
                     /translation="MTAIGEMDEKKHAKFQGRLVFVGFGSIGQGTLPLILRHINMARE
                     RISIITADDRGRKEAEQYGIKFTVNPLKRDNYQKILEPMLGRGDFLLNLSVDVSSAVL
                     IEYCLQRDVFYLDTCIEPWHGMYTDGSLPPSKRSNYGLREEVLALKKKYPRSSTVVPT
                     HGANPGLISHWVKQGMVNIARDVWGDVAIPKTREEWGRLAMKLGVKVIHCAERDTQIA
                     EPRKQRNEFANTWSVDGFVSEGRQPSELGWGTHEKHFPPDGKRHDFGCDAAIYLTRPG
                     MSVKVRSWTPDEGPYHGFLITHGEAISIADYFTLRENGNAIYRPTCHYAYHPCDDAVL
                     SNHEMGGKNWIAQPKYRIFMDEIFEGVDELGALLMGHPKGAYWYGSQLSIQQARKLAP
                     FNNATSLQVVAGVLGAMVWILENPEAGLVDPDDLDYRRVLDIADPYLGDIIGEYTDWT
                     PLKDRGWLFKEDVDESDPWQFKNFRVV"
     gene            13547..14176
                     /locus_tag="AC16CX_01255"
     CDS             13547..14176
                     /locus_tag="AC16CX_01255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024852.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011169.1"
                     /translation="MKILIVGATRGIGRQVLEQALTEGHTVTALVRDARRLDVQHERL
                     KVVKGDILDANSVALAMAGQEAVCCSIGVKVPWIYVTVFSEGTRNLLEAMKKSGVRRL
                     ACVTGIGAGDSRGHGGFLYDALFYPLLLRTIYSDKDRQEALIRGSDTDWIIVRPGFLT
                     NGPLTGRYRVVTDLSGVAAGWISRADVAHFILQQLVAPSHLRQTPLLTY"
     gene            14302..15162
                     /locus_tag="AC16CX_01260"
     CDS             14302..15162
                     /locus_tag="AC16CX_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3011170.1"
                     /translation="MNEQNQQRPAPVEFGRLSVWRDSAGLDDKQAREHAARLELRARA
                     DSEIAVRDEYVKLLGVAPGERVLDVGCGSGAVTRTLAWRVAPGGHAVGVDASPALLEV
                     ARELAQEAGLGDAIEFKPADCRMLPFPDASFDVVVAATTLSHVPDPGRALAEMVRVTR
                     PGGRVGVFDVDGDLTLFAHPDRELTRKIVAAYSDLGWVNGLFVRGLPGLMAGLGIVNV
                     KTRGFMPLESGGYYANRAERSAEAAAQAGVITKEELARWLQVLRAELAAGRFLGGQLH
                     LFVWGTRPAA"
     gene            15298..15867
                     /locus_tag="AC16CX_01265"
     CDS             15298..15867
                     /locus_tag="AC16CX_01265"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR3011171.1"
                     /translation="MEMGRYTIPELVADLRRITAEAGNEHEILKRVRPLARRAALSRE
                     TWLEDRMYEADPEQGFGVYLLHEEPDHSLAVFAVSWLPNRGTPPHDHGTWAAVAGVEG
                     DEKNVFWERVDDRRRAGYAELRKIGEKLFKVGEVAAMPAGAIHTVWNETDKVTISLHI
                     YGKHINFTGRSQFDPEKKTETQFILKMAD"
     gene            15867..16226
                     /locus_tag="AC16CX_01270"
     CDS             15867..16226
                     /locus_tag="AC16CX_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3011172.1"
                     /translation="MIAVIFEVIPAPGRKQEYLDLAASLRPALERIDGFVSIERFASL
                     THEGKILSLSIWRDEEAVKRWRQFEGHRIAQAKGRGGIFADYRLRVASVIRDYGMFER
                     EQAPADSRAIHDSKLKL"
     gene            16248..16865
                     /locus_tag="AC16CX_01275"
     CDS             16248..16865
                     /locus_tag="AC16CX_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3011173.1"
                     /translation="MSSVVCPITLDRERLKSEVRAIYAKVAENPQGDFHFHRGPEYAA
                     RLLGYDAAELSELPTGATASFAGVGNPFLMDTVLAGATVVDIGSGAGMDCLLAARRTG
                     REGSVVGIDMTDAMLARARASAADAGFSHVRFEKADMTALPLPSNSVDVVISNGVINL
                     APDKEAVFDELYRVVRPGGRLQFADIIVGTELSEQARNNIDLWTG"
     gene            17131..17319
                     /locus_tag="AC16CX_01280"
     CDS             17131..17319
                     /locus_tag="AC16CX_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011174.1"
                     /translation="MKKPFTMLAVLVFAIVALVHLLRLVFGWEVTINGAVMPVWLSVL
                     GLVIAGGLAVMLWRESRK"
     gene            complement(17398..18357)
                     /locus_tag="AC16CX_01285"
     CDS             complement(17398..18357)
                     /locus_tag="AC16CX_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011175.1"
                     /translation="MKMIAVASLAIAAAGPAGAQQYPSKPIRMIIGFPPGGGTDIVGR
                     IVGQKLSEALGQQILPDNRGGASGQIAAELTAKSPPDGYTVMMVHIAAISILPSLFPK
                     LPYDAQKDFAPITLVAIGPNLLVVHPSIPARSVKELVALAKARPGQLHYASPGAGTVQ
                     HLAAELFKLQAKVDMLHVPYKGSGQSIVDLVAGHVHLDFDSVPPVLPHVRSGRLRALA
                     VTSEKRFSILPDIATVTEGGVPGFDMSTWWGLVAPAAVSKDVVARLQAETVKVLRQPD
                     VKEKIAFAGAETVGNTAEEFAAFIRAERAKYARIVRDANIKID"
     gene            complement(18417..19913)
                     /locus_tag="AC16CX_01290"
     CDS             complement(18417..19913)
                     /locus_tag="AC16CX_01290"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006538 - L-glutamate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019665 - amino acid fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HIR3011176.1"
                     /translation="MNVPAAPEAARPPLSEARIPDDVFAGMRRENLARWPTGAGVNFE
                     AAVERHKSLPRHKQLAWVMRQAEAERRCLTQPRGGFGTFELQKELMEVLDRDGHADIV
                     PTTTDSYTRNEQWDLAQNGIEETRKMGRSMLNGYPLVNYGVEYATRLIDAIEKPAIML
                     TGTAMPKLTGEIGYAAGYSGYLGSGIAYTVSYVKELAVEEGIRNYQYLDRLVAMYQDA
                     GVELHRRQPGFLTGTNIPPAIAIITCVLDCLLAAAQGVRNYGLEMGQTLHLVQDAAAT
                     VACREMCQEYLRKRGYDDVFTTVTLLHWMGAWPQDEAQSAALIGYGGTLAAIAGAVSV
                     TTKSTHEAFGIPTPQANAEGLRMTRTAIYLARNIRLDGLPEFEHEKELIRREVRPIVD
                     KVLEMGDGDVAVGAVRAFEAGVLDVPWSPNRGVKSRVLPARDADGYLRIVDPGLMPFP
                     RDVMAVHEERLRKRAERENIPYGPELAVSSVYEMSEPIVKLMSDRWAV"
     gene            complement(19891..21303)
                     /gene="glmL"
                     /locus_tag="AC16CX_01295"
     CDS             complement(19891..21303)
                     /gene="glmL"
                     /locus_tag="AC16CX_01295"
                     /inference="COORDINATES: protein motif:HMM:NF040745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HIR3011177.1"
                     /translation="MTAALLIDFGSTYTKLRAVDLERRSVIGSGQGPSTIQTDITTGL
                     KAALADLERRCGGLPRFKYRLASSSAAGGLRMVTVGLVRELTAEAARQAALGAGARLV
                     GTFAYRLTAADIEKIAALAPDIILLAGGTDGGNTEVVLHNAAALSRSAPPCPVVFAGN
                     RTAADGARRLLAERNAVYTENVMPEFNVLNIEPARAAIRQIFIERIVHAKGIHRAAAD
                     FDQVLMPTPAAVLEGARLVADGLPGTRGLGPLLVVDPGGATTDVHSVAGGEPAPGVIP
                     RGLPEPRVKRTVEGDLGMRHNAATIAEAAGLERLAAEAGVDADRAGALLAQVCADVEH
                     LPRTREEAAFDQALARAAVNIAVRRHCGAVETVYTAAGPVMVQHGKNLARVAAVIGTG
                     GPLAASPDPRAVLRMALADPAEPFALKPRAPKLMLDRDYLLYACGLLQSVEPQAALEL
                     ALNHLVPLDDETTDERARSA"
     gene            complement(21300..21746)
                     /gene="glmS"
                     /locus_tag="AC16CX_01300"
     CDS             complement(21300..21746)
                     /gene="glmS"
                     /locus_tag="AC16CX_01300"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF002612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HIR3011178.1"
                     /translation="MARAPRTIVTGVIGSDTHIVGNRILTMVLEKAGYKVVALGALTP
                     AADFIKAAVETNADAILVSSLYGQGELDCRGFRDLCLEAGLDPILLYVGGNLVVGKRS
                     WEETEKTYLGMGFDRAAPPGTRTEVVLEWLERDFAARERGLRKATE"
     gene            complement(21768..22754)
                     /locus_tag="AC16CX_01305"
     CDS             complement(21768..22754)
                     /locus_tag="AC16CX_01305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3011179.1"
                     /translation="MKAVAVAEFGGPEKLALIEVARPEPGLGEALVRLDYAGINFIDV
                     YMRSGHYARSHTYRTPLPMVIGMEGAGTIAGLGPGANGVAVGERVAYCLERGAYAEYA
                     VVPAWKLVKIPPDVPLPVATTFMLQGLTAHYLSHSAFALGKGHTCLVHAAAGGVGQLL
                     VQLAKLRGATVIATAGSPEKAAIAKARGADHVILYREQDFREETMRITSARGVDVVYD
                     AVGKETISRSIRSLRRRGLCVNYGGASGLVQSVEPLELAEAGSVFFTRPHLADYIATP
                     EELTDRAADLFTAWRAGQLVATVDREFPLAEAAAAHRYLEGRNTKGKLLLRV"
     gene            complement(22774..23874)
                     /locus_tag="AC16CX_01310"
     CDS             complement(22774..23874)
                     /locus_tag="AC16CX_01310"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3011180.1"
                     /translation="MQSLERITVYRLRVPLVAPYRLAFGPVEHFDTIVVECTAQDGRV
                     GLGEATVLTGYTDETIEDSWRATRELAAQLVGLDSAGAQRRVGQLVHERPFTATAFGS
                     ALEMLEGSPHLEIDREMPVPILGLLNATAEEAMHAEFERLLAEGYRTIKVKVGFDVAN
                     DLAHVKAVQRITRGKARIRIDANQGYTAEQGIAFVRALDPAGIELFEQPCAAGDWDAH
                     LAVTRVSSVPMMLDESIYGLADIERAAGLGAAAFIKVKLMKLGTLEALAQAIGRIRTL
                     GMRPVLGNGVACDLGCWMEACIAARHIDNAGEMNGFLKARAQLLSEPLELRAGAVLLK
                     PGFAPRLAIEQIKPFIVETQTASARATVGANG"
     gene            24128..24744
                     /locus_tag="AC16CX_01315"
                     /pseudo
     CDS             24128..24744
                     /locus_tag="AC16CX_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            25015..25209
                     /locus_tag="AC16CX_01320"
     CDS             25015..25209
                     /locus_tag="AC16CX_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011181.1"
                     /translation="MDPTLEEPAQNTRADYGLEVMCASWNPLVAALAEPMEAYRDLVA
                     ELKAVDVESFLASFYRSQQG"
     gene            25342..26256
                     /gene="dapA"
                     /locus_tag="AC16CX_01325"
     CDS             25342..26256
                     /gene="dapA"
                     /locus_tag="AC16CX_01325"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3011182.1"
                     /translation="MAALQGSLVAIVTPMQEDGALDLEAFRRLIDWHIKEGTDGIVVV
                     GTTGESPTVDFDEHRLLIKTAVDHAAKRVPVIAGTGANATSEAIELAAYAKEAGADYS
                     LSVVPYYNKPTQEGLYRHFKAIAEAVDLPMIVYNVPGRTVADLQNETVLRLAQVPNIV
                     GIKDATANLERGSDLLRRKPRDFRVYSGDDATGLALMLLGGHGVISVTANVAPRLMHE
                     MCAAALAGEVVKAREINHRLLPLHRNLFLEANPIPVKWAVQQMGLIKGGIRLPLTPLS
                     AACHEPVREAMRHAGIRADVKEGRYAAA"
     gene            26243..27397
                     /gene="bamC"
                     /locus_tag="AC16CX_01330"
     CDS             26243..27397
                     /gene="bamC"
                     /locus_tag="AC16CX_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HIR3011183.1"
                     /translation="MPPRKVVLCLLAVLLGAVLGACSYIGLESKKIDYKSAGKLPPLE
                     VPPDLTRPNTDDRYVVPDINPKSTATYSEYSRERSGQRTSTGGSSVLPQLDSARIERD
                     GSQRWLVVKGAPDQVWPVVKDFWQEAGFIVNLEVPESGVMETDWAENRAKLPDIGLIR
                     GFLSKMLDTIYSTGERDKFRTRLERGSQPGTTEIYISHRGMEEVYTSSTSSSDAQPDT
                     RWQPRPPDPNLEAEMLGRLMVRFGVEKSRVQAQLATGASAPQRATLSKGSDGAGTLAL
                     DDQFDRAWRRVGLALDRVGFTVEDRDRSKGLYFVRYIDPQIDNKTADNKGWLSRLKFW
                     GSDKPKQEQYRIEVKDVAPGSQVKVLSKDGAQENSDTAGKILTLLYEQLK"
     gene            27509..28276
                     /locus_tag="AC16CX_01335"
     CDS             27509..28276
                     /locus_tag="AC16CX_01335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3011184.1"
                     /translation="MRFASLGSGSQGNALLIESGRTRILLDCGFGLNEVTARLSRLGL
                     APGDLDAILVTHEHEDHGGGVARLSVRYDIPVYLTRGTLSAMGAEGRAIANRILIDAY
                     TPFAIDSIEVRPYTVPHDAREPVQFVLSDGAVRLGVLTDTGHPTPHIAQALSGADALV
                     LECNHDLDMLMNGPYPAQLKSRIAGRLGHLANKASAEIVRAMDCSRLQHVIAAHLSQT
                     NNTPELARAALAGALNCAPEWIGVATQDEGFGWREIR"
     gene            28755..29054
                     /locus_tag="AC16CX_01340"
     CDS             28755..29054
                     /locus_tag="AC16CX_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3011185.1"
                     /translation="MLPVSVVKALASERRLQILTWLKRPTVHFPPQVDGDLVRDGVCG
                     VFIAKKLRVSQPTASEHLRILTHAGLVKAKRIKQWTFYKRDERRIAEVKRAFRSA"
     gene            29082..29459
                     /locus_tag="AC16CX_01345"
     CDS             29082..29459
                     /locus_tag="AC16CX_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011186.1"
                     /translation="MPREAYTTDKIAAPVGPFSPGVRAGEFVYISGQVAQDPGTGRLV
                     AGDVGVQTEQIFRNLGPLLHSAGKTFSDVVKASVFLTDMRDFPAMNAVYGRHFEAPYP
                     ARTTVGVAALPLGAAVEIDLVLR"
     gene            29478..30383
                     /locus_tag="AC16CX_01350"
     CDS             29478..30383
                     /locus_tag="AC16CX_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3011187.1"
                     /translation="MSHRFVIADVFTEIAFGGNQLAVFPDGRGLSDRAMQALAREINF
                     AETTFVLPPKDPRHTRRVRIYTPKMELPFAGHPTVGTAAVLTRLGLVQISGGATTIVL
                     EEGVGPIAVEIQVRGEATFARLVTEKEVESPSTRPARNAVAAVLSLPEEAILETWFAS
                     VGVPFCFVHLAAKETVDRAVLDRVAWSANFANAWSPNLYFFAGGLVPGSRLYVRMFAP
                     ALGIEEDPATGSGAAALAGCLAARSPDQEGTFKWEIDQGVAMGRPSVIEASAEKVHGR
                     TVKVKVGGATVLMGEGMMTVPPGYA"
     gene            30477..31184
                     /locus_tag="AC16CX_01355"
     CDS             30477..31184
                     /locus_tag="AC16CX_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3011188.1"
                     /translation="MNNTALVRSFSRLSTPLICDACLRLGLGYRMPTHGIRPLRPVMR
                     IAGRVLPARHYGSVDVFLEALEGATAGDILVIDNGGRLDEACIGDLIVLEAKGASLGG
                     IAVWGVHRDSAELALIDFPVYSYGSCPSGPQRLDARPSDALVSASFGAFRVSSSDFVV
                     GDDDGLVFAPLSEAGRIFATAESIWATEHAQAEAIRSGTTLRAQLSFAEYLEARKRDP
                     SYTLRMHLKRIGGAIEE"
     gene            complement(31354..32040)
                     /locus_tag="AC16CX_01360"
     CDS             complement(31354..32040)
                     /locus_tag="AC16CX_01360"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR3011189.1"
                     /translation="MPPVTAVSHLGDYGPSLVAFGLFSFLHSIGAQEPFKNALARWTS
                     PFFVEHFWRIVYCALSFVALYYGVAAFHWARHPENDIWLIAYPEWLWQAVTVLHLGSI
                     ALLYVAFLQSDYLEFLGLKQAWRGLPALLGMPVRPVTVELFGTHRLVVKGVYGWVRHP
                     MMVGGLLFLLTSGPSVNNLIYTAMYAAYMLIGGYYEERRLVRIFGEDYLRYQARVGAF
                     FPRLWRQPAA"
     gene            complement(32059..32538)
                     /locus_tag="AC16CX_01365"
     CDS             complement(32059..32538)
                     /locus_tag="AC16CX_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3011190.1"
                     /translation="MISKLLLAIALTLALVSPAMAAGSSSTSRPSAPPRAQPSDYDLG
                     LKAVQAGDYQRALTLLQKVVQAEPRNADAWNYVGFSHRKLKQFDQSLAAYQKALAINP
                     DHRGANEYLGELYLMTGEPQKARERLAKLQSLCPRGCEEYEDLKKAVEAYQSVQKKG"
     gene            complement(33194..34285)
                     /locus_tag="AC16CX_01370"
     CDS             complement(33194..34285)
                     /locus_tag="AC16CX_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3011191.1"
                     /translation="MKITNIQTQIVRLPADEPLAGGPVTPGATRDMVTLRLGTDAGIE
                     GIGYTFFGAALTGALKHAVDALGNLAIGEDPFRTEAVAAKLRTTAAGSGPGGILTLAL
                     SAIDIALWDIRGKALNLPLWQLIGGSRESVPTYASGALMRTFSLEHLVKAAPRLAEKG
                     FRQMKTQLALPGDTSPEREVERVRRVRESIGPDIDLMCDINQRWSVHQAIEIGRRIED
                     VHLYWLEDVTAHDDYPGLARVADALSTPVCGGEYLYGPVPFRHMLEARSVDIVMIDLM
                     RAGGITQWLKIAGMAEAYNLPVVSHLVPEIHVHLIAAVPNGLTVEYMPWTFPLFEEVP
                     VPVKGELAVPKKPGLGLKFDEAALKRFAA"
     gene            complement(34420..34680)
                     /locus_tag="AC16CX_01375"
     CDS             complement(34420..34680)
                     /locus_tag="AC16CX_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011192.1"
                     /translation="MKRSSILIATLVAIGLGACGEPQKPAPKTTTSGPAKPTEAAPAP
                     PPPPSPTAEAPKPEATVAAGAPAPTAPAGDMKKEEMKKEEKK"
     gene            complement(34901..35428)
                     /locus_tag="AC16CX_01380"
     CDS             complement(34901..35428)
                     /locus_tag="AC16CX_01380"
                     /inference="COORDINATES: protein motif:HMM:NF051921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7931 domain-containing protein"
                     /protein_id="HIR3011193.1"
                     /translation="MTGDEKTPAVELAGNLPNAEYRRFEGMREYEAVIDELIPQTQRV
                     IRVFDMSLSRAWNTAQRYEVLRQFLLASRSNRLLIVVHDAETIERECPRLVDLVRQFG
                     AGVKIHETLSPAKQVYDPYVVFDANHYVHRFHYRYLRAAQGTNDLVGAQQLLDRFAEI
                     WEVSVLAVTASTSGL"
     gene            complement(35439..36563)
                     /locus_tag="AC16CX_01385"
     CDS             complement(35439..36563)
                     /locus_tag="AC16CX_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JmjC domain-containing protein"
                     /protein_id="HIR3011194.1"
                     /translation="MTKLGLAGLSAGEFLRRYWQKKPLLVRSALPQCADIVQPRALLA
                     LAERDDLESRLVQREGRRWKVRHGPFDQRMLGRLPKSGWTLLVQGVNHALPAARKLLD
                     EFAFIPYARLDDLMVSYAPPGGGVGPHFDNYDVFLLQGEGRRRWRVSRQCDLELVAGA
                     PLRILKRFRPAREWVLHTGDMLYLPPRWAHDGIAADPCITYSVGFRAPDAQELGARFL
                     DYLQDHLQLPGIYADQGLKPPRHPARLGDHMVRKVRGFLQSIRWSGGDVARFLGCYLT
                     EPKAHVVFARPLRPLPKRTFSASAARHGVRLSLPTRMLFRGDIVFINGEACPVGAPAA
                     RLLARLADHRMLPPPLRLVREAAQLLYQWYRAGYINFGDD"
     gene            36848..37354
                     /locus_tag="AC16CX_01390"
     CDS             36848..37354
                     /locus_tag="AC16CX_01390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005435492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3011195.1"
                     /translation="MLPETLHTATMLQPGQPAPGFDLPDADMNVVSLESFRNRRNVVL
                     YFYPKDDTPGCTLEAIEFSEKEDDFEALKTVVVGVSMDDCLTHGAFRDKHGLSVQLLA
                     DAEGEVCRRYGVLHDKEVDGRKKTCIVRSTFIIDRKGVLKHALYGVNPRGHAQEILGL
                     LKGIGRCK"
     gene            37345..37824
                     /locus_tag="AC16CX_01395"
     CDS             37345..37824
                     /locus_tag="AC16CX_01395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3011196.1"
                     /translation="MQIANNTVVSLTYELLDTDGKTIEKTDAPIEYLHGGHDGIFPLV
                     EKALAGKSTGDSCRVRLEPDDAFGEYDAELVRLEPRSKFPGNIAVGMQFEGRGAESGA
                     TLIYTVTDVAEDKVVVDGNHPLAGRTLEFACTVTAVRAATGEEITHGHVHGDGGHHH"
     gene            complement(37903..>38300)
                     /locus_tag="AC16CX_01400"
     CDS             complement(37903..>38300)
                     /locus_tag="AC16CX_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011197.1"
                     /translation="RLMPEEFRRRSTTGGKWLEQSYSLARANGLRAVVILMQGNPWAS
                     PTGRYFGYRELLAALASETREFHGEVLLVHGDTHRYRVDRPLRDSATGATLANFTRVE
                     VFGSPSMNWVRIRVREEDRRVRFEVTPGS"
BASE COUNT         6828 a        12053 c        12386 g         7033 t
ORIGIN      
        1 gactgcgtcg ttcggcagcg acggcagcgc tacagcggca gccgcgccgc cttgcagctc
       61 gacgctcgca aggcggggcc tgctgtcgta ggtcgagtgc cagagatgct tcccgtcaaa
      121 atcgaaggag accgccagcc cctccgcgcc gcgcgcgagc ggctcgaagc tgtcgcccga
      181 gtttctcgaa acgtagatgc cggatgaggt cgccgcagcg atggtctttg cgtcactggg
      241 atgcaccgcg agcgcccgtg gcttgcccgc gagtcctttt gccgccgcgc gctgccacat
      301 gaagccgtca ttgaccgtgt agtagagacc cggcccttgc atgcgcgagt ttctttctga
      361 gttccagacg tacaccgcat tcgtgttcca gctcgcggcc atcaggtgga aatcggactc
      421 gccctcgagc gagagcttct cccagttctt tccgccgtcc gcgctgcgga tcaggccgaa
      481 cggatcgatg agcgcactac cctgcgccgg atggccgctg ctgtagagac gggtgcgggt
      541 cgcagcgaag cccatgtaat cgtgcgcctg gccggaggct ttcgaccact taccggccga
      601 gtacaccgca agaccatggt ggctcggcac catgagccgc ttgccatcgt ggctgaaagc
      661 gagcccgtgt acgtggtgaa gcgtcaccac aggctgcgcg cctccctcgg ctgtggtcag
      721 aaacgcaaac gcggccgccg ttaccagcgg tgccgcgagc tttcgcgttc gttcaagtac
      781 cgctttcatc atttctctca tgtcaggttg gaacaatctg tctttcacga ttcgatttgc
      841 ggccagaatt cctcgggtgt cgcttccgac cgcgtgcgga tgagaaagag cttttcgacc
      901 ggcatcacgg cgacaatgcc gtcgccgggc agtccggtat gggcggcgtt catgatcgct
      961 gccgttatgg cttcgacctc gtcggtgcgg gtatagattt cgatcctgac ctcttcgacc
     1021 atccagctcc gctcgaagaa gtcgtgatat tcaccgtaac ctttgacctt tgaaacgttg
     1081 atgcgctcga cgcctatgtc ctggagcgtc ttttcgacct gctccagcct gtcgcggcgg
     1141 atcatcgcaa cgatcaattt gatgtccatg gtgatctcct ggggattcca cattcagctc
     1201 gcggtccgcg atttcggtgc cgccacggtg ccgctgcggg taatcatggc tcccttaact
     1261 agcgcataaa tcgctggtat cacgatcagc gtcagaatcg tggccgagat catgccaccc
     1321 accatcggtg cagcgatgcg gcgcatcacc tccgagccgg tgcccgtgcc ccacatgatc
     1381 ggcagcaggc ccgcgatgat cgcaaatacg gtcatcatca gcggtcgcac gcgtaacacc
     1441 gcgcctcgca tgattgcatc atcgagatcg gcgatcgtca cgagttctcg cgccgcatgc
     1501 cgtctggcgc ttgccgcgga gaaggcctgg tcgaggtaga tcaacatcac cacgccagtc
     1561 tcggctgcga cacccgccag cgcgatgaac cccaccgcga ccgcgacgct catgttgtag
     1621 tcgagcaggt acatcagcca gattccgccc acgagcgaga acggcaccga ggccatcacg
     1681 atgagcgtct cggtaaggcg gttgaaattg agatacaaga gcacgaagat cagcaggagg
     1741 gtcaccggca cgacgacttt catgcgctcc ttggcgcgct ccaggtattc gaactggccg
     1801 ctccacgtgg cgtaatagcc cggcgggaat ttcacctgct cgcgcacggc tttctgcgcg
     1861 tcggcgacgt aaccgccgat gtcacggttg cggatgtcca cgaagatgta cgccgagagc
     1921 agggcgttct cggtgcggat tcccggcggt ccctgcgtga gcgtcacctg cgcgacctgg
     1981 ccgagcggca ccatggcgcc gttcatgacc ggcaccagga cctgggtggc gatcatccgc
     2041 ggatcgttgc gcagctcgcg gggataacgc acgatgacgc cgaaacgctc gcgcccctcg
     2101 accgtggtcg tcaccatctc gccgccaagg gcgatggcta cgacttcctg aaggtcgccg
     2161 accgccagcc cgtagcgcgc gagtgccatg cggtcgggct cgacgttgag gaagtagccg
     2221 ccggtgatgc gctcggcata ggcgctggtc gtgccgggca cggtcttcac gaccgcttcg
     2281 atctgcttgg ccagccgttc catctcgccg aggtccttgc cgaacacttt gatgccgatc
     2341 ggcgtgcgga tgccggtgga gagcatgtcg atgcgggcct tgatcggcat ggtccaggcg
     2401 ttggagacgc ccggcatctg caccgcccgg tcgagctcgg cgatgagcct ctccaccgtc
     2461 atgccggacc gccactcgga ttgcggcttg aggttgatca cggtctcgaa catctcgatc
     2521 ggcgccggat cggtcgcgct gttcgcgcgg cccgccttgc cgaacacgga ggcgacttcg
     2581 ggaaagctct tgatgatgcg gttctggatc tgcatcacct ccgccgcctt ggtcaccgac
     2641 atgcccggca ggctggtcgg catgtacatc agcgtgccct cgttcagcgt cggcatgaac
     2701 tcggtgccca gttttgtcgc tgggtaaagc gtcatcgaca aaactaagcc tgcgaccacg
     2761 agcacccatg cccgatggcg caatgctgta gagaggaccg ggcgataaac tgcaatcatg
     2821 attctgctta aaggattcgc ggattcactg cgaatccttc cgcggatgaa cagcagcatc
     2881 agcaccggga ccagcgtgat cgagagcaag gccgcgcccg ccatggcgaa agtcttggta
     2941 tacgcgagcg gtgcgaacag gcgtccttcc tgcgcttcca gcgtgaacac cggcagaaac
     3001 gagatcgtga tgatgagcaa cgagaagaac agcgccgggc cgacctcgcg gcaggccgta
     3061 atgatcgctt ccaggcgtga tgctccgggc tcgaggcgtt ccaggtgttt gtgcgcattc
     3121 tcgatcatca cgatcgcggc atccaccatt gcgccgatgg cgatcgcgat tccgcccagg
     3181 ctcatgatgt tggagttgag cccgaggaag cgcatcgcga tgaacgcgat cagcacgccg
     3241 attggcagca tgatgatcgc gaccagcgcg ctccgcacgt gcaggagaaa gacgatgcac
     3301 acaagggcca cgatcaggct ttcctcgatc agcgtccgct tgaggttgtc gatggcgcga
     3361 tggatgagat cggagcggtc gtacaccggc tcgatggcga cgccgggcgg caggccgctc
     3421 gcgatctcct cgatcttgga cttcaggttg gagatcacct cgagggcgtt ctggccgtag
     3481 cgcgccatcg cgatgccggc gacaacttcg ccttcgccgt tcaactcggt gatgccgcgc
     3541 cgctcgtccg gtccgagctc gatccgcgct acgtccctga gcatcaccgg gatgccgccc
     3601 tcggatttca ccaccagctg ttccaggtcg gacacgccgc gcaggtagcc gcggccgcgg
     3661 accatgaact cggtttccgc catctccacc acgcggccgc cgacgtcggt gttgctcatg
     3721 cggatgacct gcgcgatcct cgacagcggt acgccgtagg cttgcagctt gcgcgggtcc
     3781 acgatcacct ggtactgctg gacgaaaccg ccgatgctcg ccacctccgc cacgccctgc
     3841 gccttggtga gctgatagcg gatgaaccag tcctgcaaag ttcgcagctc cgcgagcgag
     3901 cgcttggcgc cgatgacggc gtactgataa acccagccca cgccggtcgc atcgggcccg
     3961 agcgagggtg tgatgccgcg cggcagtcgg ccggaggcga agttgaggta ttccagcacc
     4021 cgggagcgcg cccagtagat gtcggtgccg tcctcgaaga tgacatagac gaaggaagcg
     4081 ccgaagaacg agaacccgcg cacgaccttg gagcgcggca cgctcaacat ggcggtggtc
     4141 agggggtagg taacctggtc ctccaccacc tgcggcgcct ggccggggta ctcggtatag
     4201 atgatcacct ggacgtcgga gagatccggg atcgcatcga gcggggtctt ggcgacagcg
     4261 tagatcccgg cgccgacgat gaacgcggtg ccgagcagca ccaggaacac gttgcgcgcg
     4321 gaccattcga tgatgcggtt cagcatgtca gtgcctcttg tgctcggcag cggccgcgcc
     4381 cggcgcgatt cgcgtgatca cgaattcgcc cggccctctc tgcacgatgt cgaattgcac
     4441 gctctggccg ggtctcacgg tcgtgagcag cgccttgtcc tcgacccgga aatccatcgt
     4501 catggccggc cacttgaggc tcgggatttc gccgtggctg acggtgaccg ccgcgcctgt
     4561 atagtcaatg ctctccacgg tgccctggcc tttatgcacc tgcactgtgc ccggcgtcgc
     4621 ctccggcgcc cctccagggc cggcagccgc gccgaacgtg gcgagcgcag ctttgagatt
     4681 gctctcggag tcgatcagga aattcgcgct taccaccacc atctcgccgg cctgcactcc
     4741 ctccagcact tcgacgtagc cgtcggcgtg cgcgccgagc ttgacggggc gcggctcgaa
     4801 ttgcccttct ccgcgctgca ccagcacgat ctggcgggtg ccgctatcga gcaccgccga
     4861 gtcgggcacg gccaacgcct ttgtcttcat tcggcctgcg gcgagctcga cgctggcgta
     4921 catcgccggc ttgagcaggc cgccggggtt ggacagctcg atgcgcacct tggcggtccg
     4981 cgtctcggga gtgaccgtgg gatacacgaa cgcgaccttg cccgtgaaca ccttgccggg
     5041 atacgcgttg accgtgatcc tcgcggtctg tcccggctgc acgtgcgcga gatcctgctc
     5101 gaagatgtcg gcgagcagcc acagcgagga caggttagag atcttgtaga gcacctcgcc
     5161 gggcatgaag cgcatgccct tcagcgccgg cttttccagc accacgccgg cgaccggcga
     5221 gcgtagcatg agcgtttgcc gggcgcgccc ctccttttgc agctgcttca gctcttcctc
     5281 ggagatgtcc cagttgcgca gccgcgtgag agcgctcgca acgagttggc gcatgctcgc
     5341 ctgtatttcc ggggtgccgt ccttcaccga ctccacgcct ttccaggcga tgaggtattc
     5401 ctgctgcgcg gtgacgagat cggggctgta gacttccatc agcgcctgtc cgcgcgcgac
     5461 cggctggccg gtcgtgttga cgtgcagccg ctcgatccag ccctcgaatt tcggggcgac
     5521 ggcatagagc tggcgctcgt cgatctggac ggtgcccacg gcacgcacgg tgcgaacgag
     5581 ctcgcgcgca gccgccgctt ccgtcttcac ccccagcttt tgcaccttct cgatgctcac
     5641 cttgaccacc ggtccggtgg attccggctc ctcgccctcg tagacgggga tgtagtccat
     5701 gcccatctgg tctttcttgg gcaccggtga ggtatccggc aggcccatgg gattgcggta
     5761 gtaaaggatc tttctcttgc tttctgcttt ttccgcggtt ggggtgactg acgctccttc
     5821 aactttccat ctcccgcctt ccgccttctg gctgccccac cagtagcccg cggcaagggc
     5881 cgtggcgatc aggacgacta cgaggattga attggctatt cgattcatga tgtatctcca
     5941 ggtgcgcgaa cgcgctccag ttggaactca aagcggcgat ttgccggtca gcagatcgat
     6001 ctcggcaagc gccttgttgt aattgacgac cgccgctaca tgagcgacct cgtagctgaa
     6061 caccgttatc tggttgtcca gcagcgtcat gaagtcgacg cggttgaccc ggtacgcggc
     6121 aagcgaggct tcgaccgtga gcctcgcctg cggcaggata ttgccctggt agagtcgggc
     6181 cgatcgcagg ttctgttcgg cgctcgccac ctgctggcgc agcttcgcgg cgagctcgtt
     6241 gcgctgcgcc tcgtgcatgc cgagcgcctg ctcgtgcatc gcgcgcgctt ccgcgacccg
     6301 cggcgcggtc ttgctctcgc gccacaccgg caagttgatc gccacggtga agttcaccat
     6361 atcggagcgc ctggtgccgt cgggcatgtt ttcgcgttgc ccgtaggaaa aacggacatc
     6421 gaaatcggga aagtattcct tgtgcgcaag ctccaaggtc cgttcgttgc gggcaacgat
     6481 gctctgcaac gcaagcagct gcgggcgttg cctgaacgct gtctcgcgca gcgtgtcgag
     6541 cgcgagcgaa accgggcgca gctgcggcgc ctcgggcaac ggggcggcgg tgccggtgtt
     6601 gcggccgagc gcccggttga gctcggcttc gatcatcggc cgctcgcgcg cgagcttgat
     6661 cagctcgtcg accatctttg agagctgggt ctgggccttg agcacgtcgg cctgattgcc
     6721 ttgcccgacg gcgtagcgcg cttcggtgat cttcaggaac tgctcgagga tgatcttgtt
     6781 cttttccgtg aggcgggtgg tttcgatgac cagggcgagg tcaagatagc ttgccctggc
     6841 gtcgcgggcc acccggttga ccgtctcctg gtaaccgtgc gccgcggagt ccgcgtcgcg
     6901 gctggccacg tcctggcgca gcccgcgctt gccggggtag gggaagcgct gcgacaggcc
     6961 gatcatcttc atcgtcatgt cttcacggtc gaatcggaac gagttggttg gcacgttgag
     7021 cacgccggct tccagcatcg gatcgtcgag cgtgcccgcc ggcgcgaccc tttgctgcgc
     7081 cgcgtcgcgt tcccgcgcgg ccgcccggat ctccggattg ttctgcagcg cctcgcgaac
     7141 gacagcctgg atcggcgtcg tgccttgcgc cgccgcatcc atgatggtct gaggtgcggc
     7201 caaaagcatc aggaccggaa ctaaggcgcg cagttgcatg ggcacggcgc gcgtcggggt
     7261 gaaacgcata aatcctccac agtcaaagat cgaaacgacg gcggccaaga ccgccgtggc
     7321 gaaacgacgc ggaggatcta gttacgcagg cagcagtgca ggatggctag aggaggggat
     7381 tcgatgcgtg cgagtcccgg cgcgacggcc ggcaacgcgg cgtcatcggt gctgaaggaa
     7441 atggcacgca ccgcgaacac cggcaaagcc gctgccggcg gaagatccgg cactgcggac
     7501 gggggcgaga cggcctgcga caggcatctg ccgggctggt aagcatcctg attcgtgcct
     7561 gaaacgtcat ccccgacggc gctgtggcat ggcgtctgcg ccatggcggc tgccggctgg
     7621 tcggcactcg gggcacaggc gcgcgcaagc gcggcgctct ggcacagaag caccagcacg
     7681 gcggcaacac ccgccaccgc gcgtcttgtt agtctggaaa gaaccatgcg cgggcatgct
     7741 agccgcgaaa agagggtaat gcaaacggcg caccccgccg ggtttgcgct agatcaagcc
     7801 cccgtgttgc ggtgagccaa caaatggcgg gtagattagc gtttcagtag cttgaatctg
     7861 atcagcgccc gagccgggcc ttcaacgccg cccccacatc ggacggcgta tccagcacgg
     7921 tgaccccggc ggcttcgagc gctgagcgct tggaggcata gcttcccttg tcgcccatga
     7981 cgatggcgcc ggcgtggccc atcttcctgc cttgcggcgc ggcgccgccg gcaatgaagg
     8041 cgaccacggg cttggtcatg gttctcgcgt aatcggcggc ttcctcttcc atcgagcctc
     8101 cgatctcgcc caccatgacg acggcctcgg tccccggaaa attatcgagg gcgacgagcg
     8161 cctcgcgcgt ggtggtgccg atgatcggat cgccgccgat tccgatgaag gctgattcac
     8221 cgtagccggc ctggacgacg ttgaggcata acagcgtgcc caggctgccg ctgcgggaaa
     8281 ccacgccgat cgagcctttc ctgaagatgt gtgggttgaa ggcgggcatg aagccggcga
     8341 agcactcgcc gggcgtcacg gcgccggccg tgttggggcc gatgatgaac gcgccgcgct
     8401 ccctcgccgc ggcgacaaag tacatcacgt catgcaccgg aatgtgctcg gtcagcacga
     8461 cgaggccctt tgcgccggct tcgatcgcgt ccatggcggc ttccttgacc atcaggggcg
     8521 ggatgaagag caccgcggct tcgaaaccgg ctccattcat cgcgtcccgg gcggtggcga
     8581 aaaccggcac gttgcagcgc atggtgcctg ccttgttcgg attggtgccg ccgacgaccc
     8641 gggtgccgta ggcctgcatg cgttcggtcc agaatgtgcc ctgcttgccg gtgatgccca
     8701 tcactatcac gcgatcagtg ccgcggacga tcatcgggcc gcctctgtca cgtgatcgat
     8761 atgccatggc tgccgcatcg gcagtcctgc gtcattcgac ggtcgcaccc gcaagcgggc
     8821 acctgctccg cgtctcatgc cgctgcctcc actgccgctt tgctcgcctc atccatggtc
     8881 gtaagcggcg tgacgcccaa acggtctttc aacatccgga tcgcttcctc gtcgccggtg
     8941 ccgtgcaccg aaaagaagat cggcagtctg ggtttcaagg cttcgatcgc ctcgatgacg
     9001 cctccagtca tcacatcggt gcgcgcaaac gcgccgcaga agttgatgac caggctcttg
     9061 ataccgggct tcgaaagcag cagctcgagc gcgggtttcg cctgggtgta ggcctcgcca
     9121 ccgatctcga gaaaattcgc ggggcgcccg ccgtggtggc ggatcatgtc catggtcgtc
     9181 atcgtcaggc cggccccgtt ggcgagcacg ccgacatcgc cgtcgagctc gatgtacttg
     9241 agcccggtgg ccttggctct ggcctcgagg gcggtcagct tctcgggcgt gcctccaggc
     9301 gcgatgtcgg gatggcggac gatggcgcaa tcgtccatca cgaacttgca gtccagcgcg
     9361 acgacgcggc cgtccctcgt gacgaccagt ggattgatct ccagaagctc ggcgtcgaag
     9421 gcggcataag cctggtagag cctggcgagc gcgtcggcga ccttgccttc ggccgcgccg
     9481 agccccaggc cccggaccag cgtcctggca tccgcatgag aaaagccgcg gcggatatcg
     9541 acgggctggc ggcgcagctt gccgggcgcc gtcgccgcca cttcctcgat gtccatgccg
     9601 ccctcggtcg aaaacatgac gagcgggccc ttggtcgagg gatcgttgag caccgcggca
     9661 tagagttcgc gttcgatcgg caattgctct tcgaccagca ccttctcgac cttgtggccg
     9721 gcgatctcca tgccgatgat ggcttgagca tgggtcctgg cttcgtccgg tgtcgcagcc
     9781 aaccggatgc cgcccgcctt gccgcgcttg ccggccggga cctgggcctt gacgacgacg
     9841 gcgccaattc ccgccgcgac ctcggcagcg tcgtcgggcg ttttcgcgag cccgcccctg
     9901 ggaacggcga tgccatgccg ggccaaaagc ggcttggccg cgtgttcctc gaaattcatg
     9961 agctgttctc ttctaccggc cgcgcgcgct ggtggggaaa gtgctggacc gatcgcatac
    10021 gggaaggatg aaggatgagg ggtgagccgg gaattcgatg aaagtgcctg cgcactttcg
    10081 agccggcgaa cgcccgccgc gatgcagcgc ggtgggccgg ggtcactgac caaggatgat
    10141 ccccgcagcg cgggagcaaa aaccaggtca tccttccgcc ttccgccttc atcctttggg
    10201 cggcgatgct atagaccatg cttcttgcag tagtgtccga atatatcgtc gagtatgggt
    10261 tccctgggcg ggatcgtacg gacgatccgc gtgatgttca tgaagtgctt gtagtggaga
    10321 ttgtcggaaa agctgttgcc gccccaggtg ccgcaaccca tggagagcga gaacggcatg
    10381 ccgttgtcga agctgccgcc ggtggcgaag gtgtgcgcct gtttcacgat cacgcgcgcc
    10441 acggtgagct cgtgggcaag cttgcggatg tgcgcctcgt tctcggtgta gatgccgcag
    10501 gagtggccgt tgccctggta gtcgtaaatg gcgcgcacga tgtcgaacgc atggtcgaag
    10561 tcgcgcgcgc ggtaaacggc gaacacgggc gaaagtttct cgccggagaa aggatggctt
    10621 ctgcccgttc cggtttcctc caccagcagg catttcgcgt tgacgagatc ggggcgcttg
    10681 aggccggcca cggcgcagat cttcttcact ggttgcgcgg tcaccgcggc cgagagcatg
    10741 ccgccgggga acatcgcttt ctgcagcgcg gctttttcat cctgggtcaa cagagcgccg
    10801 ccttccttcg tgagcgcctt gatcatcttg tcatagaccc gtccttggat gatcaccgag
    10861 ttttcggagg aacagctggt cgcgttgtcg aagatcttgg aagccatgat cttcttagcg
    10921 gcgtcggcgc agtcggcgct gtcgtccacg attaccgcca cgttgccggt gccgacgccc
    10981 agcgcgggcg taccgctgga gtagccggca cgcacgttgt tctgcgagcc cgtcaccacc
    11041 accaggtccg cctgcttcat gagctcggtg gtgagttcgc gggtgactgg cgagggcaga
    11101 tgctgaacca ggtcaaccgg cgcgctgatg cgcttcaact ccgcgtgcac gtacttgagc
    11161 agcagcgtgc atgtgctgta gcccttgggc gagggcgaaa cgatcacggc attgccgcat
    11221 ttcaacgcgt tgatgatgtt gttggccggg gtcgccgccg gattggtcga aggcaccacg
    11281 gcagccacca cgccgaccgg gcgcgcgatc tcggtgatgc ccttctcggg atattcagcg
    11341 atcacgccgg tggatttcgc ccctttcagg tcgcgcagca agccaagcgt cttgcggtgg
    11401 ttcttggcga ccttgtcgga gaccactccg agccccgtgt ccttgacggc gagctcggcg
    11461 agcacccggt tgtgctccgg attcatgatc gcccagccga ccgcggtcac ggcctcgtcc
    11521 aattgctcct gcgaggagcg ttcgaagact ttctgcgcgg cgcgcgcttt gccgatcagg
    11581 ttggcgaccg cgtttctcgc ggcggtggag tcacccggtg ctgcgccccg acttgccatt
    11641 cgactgtccc ctgaggatcc ggtaaccgaa gtccagatga taccgcttta tcgccatcat
    11701 cattccggtc gcggcgaggc gcacgcgtgt cgcggcgggc gcctctctcg gcgatgccgc
    11761 tggtgcgccg cgccgatcgg cgccggaaga ggcgacgcgc gcaaaataaa atgctagact
    11821 tcaccgaaga aaaatgacgg ccatcgggga aatggacgaa aagaagcacg cgaaattcca
    11881 aggaaggctg gttttcgtcg ggttcgggtc catcggccaa ggcacgctcc cgctcatcct
    11941 acggcacatc aacatggcgc gggagcgcat ttcgatcatc accgccgatg atcgcggccg
    12001 caaggaagcc gagcagtacg gcatcaagtt caccgtcaac cccctcaaac gcgacaacta
    12061 ccagaaaata ctcgagccga tgctgggcag gggcgatttc ctgctcaacc tgtcggtgga
    12121 cgtctcgagc gcggttttga tcgagtactg tctgcagcgc gacgtgttct acctggacac
    12181 ctgcatcgag ccgtggcacg gcatgtacac cgacggctcg ctgccgccct ccaagcgctc
    12241 caattacggc ctgcgcgagg aggtgctggc gctgaagaag aaatacccga gaagctccac
    12301 cgtcgttccc acccacgggg cgaaccccgg cctgatctcc cactgggtga agcagggcat
    12361 ggtcaacatt gcgcgcgacg tgtggggtga cgtggcgatc ccgaagacga gggaggaatg
    12421 gggcaggctt gcgatgaagc tcggcgtcaa ggtcatccat tgcgccgagc gggacaccca
    12481 gatcgcggag ccgcgcaagc agcgcaacga gttcgcaaac acctggtcgg tggacggctt
    12541 cgtgagcgag ggccggcaac cttccgaact cggctggggc acccacgaga agcacttccc
    12601 gcccgacggc aaacgccacg acttcggctg tgacgcggcg atctacctca cccggcccgg
    12661 gatgtcggtg aaggtgcgtt cctggacgcc cgacgagggc ccctaccacg gcttcctcat
    12721 cacccatggt gaggcgatct cgatcgcgga ttactttacc ctgcgagaga acggaaacgc
    12781 gatctaccgc ccgacgtgcc attacgccta ccacccgtgc gacgacgcgg tgctctcgaa
    12841 ccacgagatg ggcggcaaga actggatcgc gcagcccaag taccgcatct tcatggacga
    12901 gatcttcgag ggcgtggatg agctcggcgc gctgctcatg ggacacccga agggcgccta
    12961 ctggtacggc tcgcagctct cgatccagca ggcgcgcaag ctcgcaccat tcaataatgc
    13021 gaccagcctg caggtcgtcg ccggcgtgct gggggcgatg gtgtggatac tggaaaaccc
    13081 cgaagcgggc ctggtggatc cggacgatct cgactaccgg cgcgtgctcg acatcgccga
    13141 tccctatctc ggcgacatca tcggggaata caccgactgg acgccgctca aggaccgcgg
    13201 gtggctgttc aaggaggatg tggacgagtc ggatccgtgg cagtttaaaa atttccgtgt
    13261 cgtgtgacgc gaaaattcgg tgatgagtga tgggtgatga gccagcacct taataaaacc
    13321 gcagacgaac gcagataacc gacgataaaa taagggaaaa gattcatagg ggacgggcac
    13381 gcaagcgtgc ccgttttttt gtcgtgcgga cgattgtcat gctcccggct caacgggtca
    13441 tgcgaataat gccgctccgg cacgacaaga atgttgcgcg acacagcctg ctttcgggtg
    13501 tctaattggc tccggccgta cgccggcttt caacgaggag aaggccatga agatcctgat
    13561 cgtgggggcc actcgcggta tcgggcgaca agttcttgaa caggcgctga cggaggggca
    13621 tacggtaacc gccctggttc gcgatgcccg cagactcgat gtgcagcacg agcggctgaa
    13681 agtcgtgaaa ggcgacattc tcgacgcgaa ttcggtcgcg ctcgcgatgg ccggccagga
    13741 ggcggtgtgt tgcagtatcg gcgttaaagt gccctggata tacgtgacgg tcttctcgga
    13801 gggaacccgg aatctgctcg aagcgatgaa aaagagcggg gtcaggcgcc tggcatgcgt
    13861 gacgggcatc ggcgcgggag acagcagggg acacggaggc tttctctatg acgccctttt
    13921 ttatccgctt ctcctccgga cgatctactc cgacaaggac cggcaggaag cgttgatcag
    13981 ggggagcgac actgactgga tcatcgtgcg gccgggattc ctgacgaacg ggccgctgac
    14041 cggtcgctac cgcgtggtta cggatctgtc cggtgtggcc gccggctgga tttcccgcgc
    14101 tgacgtcgcc catttcatcc ttcagcagct cgtcgcgccg agccacttgc ggcagacgcc
    14161 gttgctgact tattgaaccg ccgaggcgcc aagaaattca aaatggaacc acagataaac
    14221 acagatgaac acggataaga attagaattc gaatcacgat tcacgaatca cgatccactg
    14281 cttcttcggg ggaggtgccg ggtgaacgag cagaatcaac aacggcctgc gccagtcgag
    14341 ttcggacggc tcagcgtttg gcgcgacagt gcagggcttg atgacaagca ggcgcgcgag
    14401 cacgctgcgc gacttgaact acgtgcccgg gccgacagtg agatcgcggt gcgcgacgag
    14461 tacgtgaaac tgctcggtgt ggcgccgggg gagcgcgtgc tcgacgtggg ctgtggaagc
    14521 ggcgcggtga cgcgcacgct cgcctggcgg gtcgcgccgg gaggccacgc ggtcggcgtt
    14581 gacgcgagtc ccgcgttact cgaggtggcc cgcgaacttg cgcaggaagc cggccttggt
    14641 gacgcgatcg agttcaagcc ggcggattgc cggatgcttc cgtttcctga cgcgtcgttc
    14701 gacgtcgtgg tagccgcgac gacactctcg cacgtcccgg atcccggacg cgcccttgcg
    14761 gagatggtcc gcgtcacgcg gccgggtggc cgcgtcgggg tgttcgacgt cgacggggac
    14821 ctgacccttt tcgcacatcc cgaccgcgaa ctcacgcgaa agattgtcgc cgcctactcg
    14881 gatctcgggt gggtcaatgg cctgttcgtg cgtggactac cgggccttat ggctggtctg
    14941 ggtatcgtca atgtcaaaac ccggggtttc atgccgctcg agtctggtgg ttactatgct
    15001 aaccgcgcgg agcgatccgc cgaagcagcg gcccaggctg gcgtgatcac taaggaggag
    15061 cttgcgcgct ggctccaggt actacgggcg gaacttgccg cgggccgctt cctcggcggc
    15121 cagcttcatc tcttcgtctg ggggacgcgc ccggctgcct gacatcaggg aagcactggc
    15181 tttgggtatc atggtttccg ccgggtgcgc cagaaactcc gttcccggct tcaaggatga
    15241 gctggggatc gagtaacaat attcgattcg attttcccgc atcctgggga gcgcgcgatg
    15301 gaaatggggc gttacacgat tccggagctg gtggccgacc tcaggcggat cacggccgaa
    15361 gccgggaatg agcatgaaat cctcaagcgc gtgcggccgc tggcgcgccg cgccgctctg
    15421 tccagggaaa cctggctgga ggaccgcatg tacgaagccg atccggagca gggcttcggc
    15481 gtttacttac tgcacgagga accggaccac tcgctcgcgg tctttgcggt aagctggctg
    15541 ccgaaccggg gcacaccgcc gcacgaccac ggtacctggg ccgcggtggc aggggtggag
    15601 ggagacgaga agaacgtgtt ctgggagcga gtggatgacc ggaggcgcgc ggggtacgcc
    15661 gagctcagga agattggcga aaagttgttc aaagtcggag aggtggcggc aatgccggcg
    15721 ggcgcaatcc ataccgtctg gaacgagacg gacaaggtca ccatttcgct gcacatctac
    15781 gggaagcaca tcaatttcac gggacggtcg cagttcgatc ccgagaaaaa aaccgagacg
    15841 cagttcatcc tgaagatggc cgactgatga ttgccgtgat ctttgaagtc attcccgccc
    15901 ctgggcgaaa gcaggagtat ctcgatcttg ccgcgagcct ccggcccgcg ctcgaaagga
    15961 tcgacgggtt cgtctcgatc gagcgcttcg cgagcctcac ccatgaaggc aagatcctgt
    16021 cgctttccat ctggcgcgac gaggaggcgg tgaagcgctg gcgccagttc gaggggcacc
    16081 gcattgccca ggcgaagggg aggggcggca tcttcgccga ctaccggctg cgcgttgcca
    16141 gcgttatccg cgattacggg atgttcgagc gggagcaggc gccggccgac agccgggcga
    16201 tacacgactc aaagctcaag ctgtaaacta ggcgggagga ggccactatg tcttctgtcg
    16261 tatgtcccat caccctggac cgggaaagac tcaagtccga ggtccgggcg atctatgcga
    16321 aagtcgcgga aaatccgcag ggcgattttc atttccaccg cggcccggag tacgccgcgc
    16381 gcctcctcgg atacgatgcg gcggagctca gtgagctgcc gaccggggcg accgcgtctt
    16441 tcgcgggcgt aggtaatcca ttcctgatgg acactgtgct cgcgggcgca accgtcgttg
    16501 acatcggctc gggggccggc atggactgtc ttctggcggc gcggcgcacc ggccgcgagg
    16561 gttcagtggt ggggattgat atgaccgacg ccatgctcgc gcgagcgcgc gccagcgcgg
    16621 ccgatgccgg tttcagccac gtgcgcttcg aaaaggcgga catgacggcg ttgccgctgc
    16681 catcgaacag cgttgacgtc gtgatttcca acggcgtgat caacctcgct cccgacaagg
    16741 aggcggtgtt cgacgaactg taccgggtgg tcagacccgg cgggcggttg cagttcgccg
    16801 acatcatcgt cggcaccgaa ctctcggagc aggcgcggaa caacatcgac ctctggactg
    16861 ggtgaattgc cggcgctctg caggttgcag agatgatcga agtcatccac cgggcgggat
    16921 tccggaacgc ccgcgcttgc cggttctttg acagtttctc cggaacgacc aaggaagcgg
    16981 tagcacgcaa gtatggcgtc cgcggcgcga acttcacggc atataaagct gcttgacggt
    17041 ggcgttttcg gcggctgttg aagccgcgga tcgtgatcag tgacggcgca tcggtggcgc
    17101 cttatcatcg ccatgttatc ggtgtgaatc atgaaaaagc cgttcaccat gctcgctgtt
    17161 ctggtctttg cgatcgtcgc gctcgtccat ctcttgcgcc tcgtcttcgg atgggaagtg
    17221 accatcaacg gagccgtcat gccggtgtgg ttgagcgtgc tgggcctggt gatcgccggc
    17281 gggctggcgg tcatgctgtg gcgcgaatcg cggaaataga gactgcgccc gcgcgttgaa
    17341 cggcgactcc tcgtctgttc gaacgcgcgg aattaaaccg gcgcggagcg tccggattta
    17401 atcgatcttg atgttcgcgt ccctcacgat ccgcgcgtat ttcgcgcgct cggcgcggat
    17461 gaaggccgcg aattcttccg ccgtgttgcc gacggtttcg gcccccgcga aggcgatctt
    17521 ctccttcacg tccggctgcc gcagcacctt gaccgtttcg gcctgcagcc gggcgacgac
    17581 gtccttgctc accgcggcgg gcgcgaccag cccccaccag gtgctcatgt cgaatccggg
    17641 cacgccgccc tcggtgacgg tcgcgatgtc gggcaggatc gagaagcgct tctcggaggt
    17701 caccgcgagc gcacgtagcc tcccgctccg gacgtgcggg agcaccggcg ggaccgagtc
    17761 gaaatcgaga tggacgtggc cggcgacgag gtcaacgatg gattgcccgc tgcccttgta
    17821 ggggacgtgc agcatgtcca ccttggcctg cagcttgaac agctccgccg cgagatgctg
    17881 tacggtgccc gcgcccggcg aggcgtaatg cagttgcccc ggcctcgctt tcgcgagcgc
    17941 gaccagttcc ttgacgctcc gcgccgggat cgatggatgc acgaccagca ggttggggcc
    18001 gatggcgacg agcgtgatcg gcgcgaaatc cttctgcgca tcgtatggga gcttgggaaa
    18061 gagcgagggc aggatcgaga tggccgcgat gtggaccatc atcacggtgt agccgtctgg
    18121 cggcgacttg gcggtgagct cggcggcgat ctggccgctc gcgcctccgc ggttgtcggg
    18181 caggatctgc tgaccgagcg cttcggacag tttctggccg acgatgcggc cgacgatgtc
    18241 ggtgcctccg cccgggggaa aaccgatgat catgcggatc ggcttggaag ggtactgctg
    18301 cgcccccgca gggcccgctg cggcaatggc tagggacgca acggcgatca ttttcatcag
    18361 gttccccatc ggcttcctcc tcgattatcc ggtgcgcgcc cggtcaagta tacgcgtcag
    18421 accgcccaac ggtccgacat gagcttcacg atcggttccg acatctcgta cacgctggac
    18481 accgcgagtt ccggtccgta cgggatgttc tcgcgctccg cgcgcttgcg caagcgttcc
    18541 tcgtggaccg ccatgacgtc ccggggaaac ggcatcaggc ccgggtccac gatgcgcaga
    18601 tacccgtcgg cgtcgcgcgc cggcagcacc cggctcttca caccgcggtt cggtgaccac
    18661 ggcacgtcga gcacgcccgc ttcgaacgcg cgcaccgctc cgaccgcgac gtcgccgtcg
    18721 cccatttcca gcaccttgtc cacgatcggc cgcacttcgc gccggatgag ctccttctcg
    18781 tgttcgaact cgggtaggcc gtcgagccga atgttgcgcg cgaggtagat cgcggtgcgc
    18841 gtcatgcgta acccttcggc gttcgcctgc ggagtaggga tgccgaatgc ctcgtgcgtg
    18901 ctcttggtcg tgacgctcac cgctccggcg atcgccgcga gcgtgccgcc gtaaccgatg
    18961 agcgcggccg actgcgcctc atcctgcggc caggcgccca tccagtggag cagcgtcacc
    19021 gtggtgaaca cgtcgtcgta gccgcgcttg cgcaggtact cctggcacat ctcgcggcac
    19081 gccaccgtgg ccgctgcatc ctgcacgaga tgcagtgtct gccccatctc gagcccataa
    19141 ttcctcacgc cttgcgccgc ggcgagcagg cagtcgagca cgcacgtgat gatggcgatc
    19201 gcgggcggga tgttggtgcc cgtcaggaaa ccgggttgcc gccggtgcag ctcgacgccc
    19261 gcgtcctggt acatcgcgac gagccggtcg aggtactggt agttgcgtat gccttcctcg
    19321 acggcaagct ccttgacgta ggacacggtg taggcgatgc cggagccgag atagccggag
    19381 tagcccgccg cgtagccgat ctcgcccgtg agcttcggca tcgccgtgcc ggtaagcatg
    19441 atggcgggct tttcgatcgc gtcgatcagg cgcgtcgcgt attccacgcc gtaattgacg
    19501 agcgggtagc cgttcagcat cgagcggccc atcttccggg tctcctcgat gccattctgc
    19561 gcgaggtccc actgctcgtt gcgggtatag ctgtcggtgg tggtgggcac gatgtcagcg
    19621 tgaccgtcgc ggtcgaggac ctccatcaat tccttctgga gctcgaaggt gccgaagccc
    19681 ccgcgcggct gggtgaggca gcggcgttcg gcctccgcct gccgcatcac ccacgcaagc
    19741 tgcttatggc gcggaagcga tttgtggcgc tcgaccgcgg cttcgaaatt cacgcccgcg
    19801 ccggtcggcc agcgcgcgag attttccctg cgcatcccgg cgaacacgtc gtccgggatg
    19861 cgcgcctccg ataggggtgg cctcgccgct tcaggcgctg cgggcacgtt catcggtggt
    19921 ctcatcgtcg agtggcacga ggtggttcag cgccagctca agggcggcct gcggctcgac
    19981 gctctgcaac aatccgcaag catacagcag gtaatcccgg tcgagcatga gcttcggcgc
    20041 gcgcggtttg agcgcaaacg gctcggcggg atcggcaagt gccatccgga gcacggcgcg
    20101 cggatcgggg cttgcggcga gcgggccgcc ggttccgatc acggcggcca ctcgggcgag
    20161 gttcttgccg tgctggacca tcaccggccc ggcggcggtg tagacggtct cgaccgctcc
    20221 gcagtgccgg cgcacggcga tgttcacggc cgcgcgcgcg agcgcctggt cgaatgcggc
    20281 ttcctcgcgc gtccgcggca gatgctcgac gtcggcacac acctgcgcga gcagggcgcc
    20341 cgcgcggtcg gcgtcgactc ctgcctcggc agcgaggcgc tcgagccccg cggcctcggc
    20401 gatcgtcgcg gcattgtggc gcatgccgag gtcgccttct acggtgcgct tgacgcgcgg
    20461 ctccggcaga ccccgcggaa tcacgcccgg tgcgggctcg ccacccgcca ccgaatggac
    20521 gtcggtcgtc gcgccgccag ggtcgacgac cagcagcggg cccaggccac gcgttcccgg
    20581 caggccgtcc gcgaccaatc gcgcgccttc gagcaccgcc gcgggcgtgg gcatcagcac
    20641 ctggtcgaaa tcagcggctg cgcggtggat gcccttggcg tgaacgatgc gctcgatgaa
    20701 gatctgccgg atcgcggcac gcgcgggctc gatgttgagc acattgaact ccggcatgac
    20761 gttctcggtg tagacggcgt tcctctccgc aaggagcctg cgcgcgccgt ccgccgcagt
    20821 gcggttgcct gcgaacacga cggggcaagg gggcgcgctg cgggagaggg cggccgcgtt
    20881 gtgcagcacg acttccgtat tgccaccgtc cgtgccgccc gcgagcagga tgatgtcggg
    20941 cgcgagcgcg gcgatctttt cgatgtcggc ggcggtgaga cggtaggcga aagtcccgac
    21001 gagcctggcg ccggcgccca gcgcggcctg gcgcgcggcc tcggcggtga gttcgcgcac
    21061 gagcccgacg gtcaccattc gcaggccccc ggccgcgctg ctcgaggcga ggcggtactt
    21121 gaaccggggc agtcctccgc agcgccgctc gaggtcggca agcgccgctt tcagcccggt
    21181 cgtgatgtcg gtctggatcg tggacgggcc ttgccccgag ccgatgacac tgcgccgctc
    21241 gaggtccacc gcgcgcaact tcgtgtaggt cgagccaaaa tcgatgagca gcgcggcggt
    21301 cattccgtcg ctttcctcag cccgcgctcg cgcgcggcga aatcgcgctc gagccattcg
    21361 agcaccacct cggtgcgcgt gccgggcggc gccgcgcgat cgaaccccat gccgaggtag
    21421 gtcttctcgg tctcttccca cgaacgcttg ccgaccacga ggttgccgcc gacgtagagc
    21481 aggatcgggt cgaggcccgc ctccaggcag agatcgcgaa agccgcggca gtctagctcg
    21541 ccctggccgt agagcgagga cacgaggatc gcgtccgcat tggtttcgac ggcggccttg
    21601 atgaaatccg cggcaggcgt cagcgcgccg agcgccacga ccttgtagcc cgccttctcg
    21661 agcaccatgg tgaggatgcg gttgccgacg atgtgcgtat cggagccgat gacgccggtg
    21721 acgatggtgc gcggtgctct ggccatggcg acaggtggtg gcgcggacta gacgcgcagc
    21781 agcagcttgc ccttggtgtt tctgccttcg aggtaacgat gcgcggcggc ggcctcggcg
    21841 agcgggaact cgcgatcgac cgtagccacg agctggcccg cgcgccaggc ggtgaaaaga
    21901 tcggccgcgc ggtcagtgag ctcttccggc gtcgcgatgt aatcagcaag gtgcggccgc
    21961 gtgaagaaaa ccgaacccgc ctccgcgagt tcgagcggct ccaccgactg gaccagaccc
    22021 gatgcgccgc cgtagttcac gcacaggccg cggcgcctga ggctgcggat actgcgcgag
    22081 atggtctcct tgccgaccgc gtcgtagacg acatctacgc cccgggcgct ggtaatccgc
    22141 atcgtctcct cgcggaagtc ctgctcgcgg tagaggatga cgtgatccgc cccgcgcgcc
    22201 ttcgcgatcg cggccttctc cgggctgccc gcggtcgcga tcaccgtcgc gccgcgcagc
    22261 ttcgcgagct gaacgagaag ctgcccgacg ccacccgcgg cggcatgcac gaggcaggtg
    22321 tggcccttgc cgagcgcgaa cgcggagtgg ctgaggtagt gggccgtgag accctgaagc
    22381 atgaacgtgg tcgcgaccgg tagcggcacg tcaggcggga tcttcacgag cttccacgcc
    22441 gggaccaccg cgtactccgc gtaggccccg cgctcgaggc agtaggccac gcgttcgccc
    22501 accgcgacgc cgttagcacc cggtccgagt cccgctatgg tgcccgcgcc ctccatgccg
    22561 atcaccatcg gcaggggcgt ccggtaagtg tgcgagcgcg cgtaatggcc gctgcgcatg
    22621 tagacgtcga tgaaattgat gcccgcataa tcgagcctga ccagtgcctc gcccagtccg
    22681 ggctcgggcc tggccacctc gatcagcgcg agcttctccg gcccgccgaa ttccgcgacc
    22741 gcgaccgcct tcatctccgc ttctttcgcc gttctaaccg ttagcgccta ccgttgcccg
    22801 cgccgatgcc gtctgcgttt cgacgatgaa gggcttgatc tgttcgattg cgagccgcgg
    22861 cgcgaagccg ggcttgagca gcaccgcgcc ggcgcgcagc tccagcggct cgctcagcaa
    22921 ctgtgcgcgc gccttgagga aaccgttcat ctcgcccgcg ttgtcgatgt gccgcgcagc
    22981 gatgcaagct tccatccagc agcccaggtc gcacgcgacg ccgttgccga gaaccggcct
    23041 catgccgagc gtcctgatgc gcccgatcgc ctgagcgagc gcttccaggg tgccgagctt
    23101 catgagcttg accttgatga aggccgccgc cccgaggcca gctgcgcgct cgatatccgc
    23161 gagcccgtaa atcgattcgt cgagcatcat cggcacgctc gacacccgtg tcacggcgag
    23221 atgggcatcc cagtcaccgg ccgcgcacgg ttgctcgaag agctcgatgc cggccgggtc
    23281 gagcgcgcgt acgaaggcta ttccctgttc ggcggtatag ccctggttcg cgtcgatgcg
    23341 gattctcgct ttgccgcgcg tgatcctctg taccgccttg acgtgcgcga ggtcgtttgc
    23401 cacgtcgaac ccgaccttta cctttatggt gcggtagccc tccgccagca ggcgctcgaa
    23461 ctcggcgtgc atcgcctctt cggcggtcgc gtttaggaga cccaggatcg gcaccggcat
    23521 ctcgcggtcg atttcgaggt gggggctccc ttcgagcatt tccagcgcgc tgccgaaggc
    23581 cgtggcggtg aacggccgtt cgtgcacgag ctggccgacc cggcgctgcg cgcccgcaga
    23641 gtcgagcccc accaactgcg cggcgagctc gcgcgtcgcg cgccagctgt cctctatcgt
    23701 ctcgtcggtg tatcccgtca gcacggttgc ttcgcccaac ccgacgcggc cgtcctgcgc
    23761 ggtgcattcg acgacgatgg tatcgaaatg ctcgaccggg ccgaacgcga gacggtaggg
    23821 cgccaccagc ggtacccgga ggcgataaac ggtgatccgt tctagcgatt gcatgcgcga
    23881 ttgctccggg gaatcgaggg gcggatggac aatccccgta gaccgcattt tacttcgtcg
    23941 cgcgcgcctc gcgcggcgcc cgtgctcgcg tgcacgacgc gccacgcgac ccggcagcgc
    24001 gtcctggtcc gcagccacga catataatcg gcagggatgc ggcgccgtgc ggcggaggct
    24061 ccgtgccgcg tcctagcttg tcttccgtgc tgcggtactg atgcgtttcg attgcgtcgc
    24121 gagagccatg gccgaagcta ccatccatca cgcaaacacg ccgtcaccgt gggtcacccg
    24181 ctgggcggac cgtgtacctg ccggggggcg ggtgctcgac atcgcctgcg gcaacgggcg
    24241 acatgcacgc ttcttcgtat cccgcggtca ccccgtcgag gcagtggacc gggatcctgc
    24301 catgctggcc agtctggtgg gtacccccgg aatcaatacc cggcacgctg acctcgaaaa
    24361 taggccttgg ccgtacgaag gggagcagtt tgccggtatc gtggtggcca attacctcca
    24421 ccgcccgctg tttccccacc tgctggctgc gcttgccccg gatggggtcc tgatctatga
    24481 aaccttcgcg gtcggcaacg agcgctatgg ccggccttcc aaccccgcct tcttgctgaa
    24541 gccgggagag ttgctggagg tagcccgcgg ccggctgctg gtaatcgcct acgaggatct
    24601 attcgtttcc gaccccaaac ccgccctggt ccagcgcatc tgcgcgataa attcgcgcta
    24661 actccccggt tcggaagggc tgccggagtc gcgcactatg actctctggg cggacgcgtc
    24721 acatctgatc gcgtgaattt ataatctcat agaatcaaat agataactgc acttcaagca
    24781 aagccaagcc tcccctctgc gccgaccgac ggaagccggg gaaaggagtc gccggcgcga
    24841 ctacattggt cgtaaacgag aggtccggtt agaatttccg ttaatttctg ggtttgttaa
    24901 ggaacgcgct cggacgcgtt ccttgtcgca atagtgcaac actgctcagg gataatgcgc
    24961 aggttccgga accattcaac catttttcag ttccatacgt tatagagtaa cgacatggac
    25021 ccgacgctcg aagaaccggc gcaaaacacc cgggccgatt acggcttgga ggtgatgtgc
    25081 gcgagctgga atccgctggt agcggcgctg gccgagccaa tggaggctta ccgcgacctc
    25141 gttgcggagc tcaaggcggt tgacgtcgag tcttttctcg ccagtttcta ccgttcccag
    25201 caaggctgag cggcttcttg ccgactgccg gcaaccatcc cgtcataaca cggtcacagg
    25261 ccttatccgc gcgcgccgga taaggtagaa ttgtggaatt tcaccggcac ccgcggctgt
    25321 tttataaggg aaattccgga aatggcggca ttgcagggca gcctggtcgc gatcgtcacc
    25381 ccgatgcagg aggacggcgc actcgacctt gaggcgttcc gccggctgat cgactggcat
    25441 atcaaggaag gcacggacgg gatcgtcgtg gtcggcacca cgggcgaatc gccgacggtg
    25501 gacttcgacg agcaccggct gctgatcaag accgcggtgg accacgccgc gaagcgcgtt
    25561 cccgtcatcg ccggcaccgg ggcgaacgcc accagcgaag cgatcgagct cgccgcctac
    25621 gccaaggaag ccggcgccga ctacagcctg tcggtcgttc cgtactacaa caagcccacc
    25681 caggaagggc tctaccgcca cttcaaggcg atcgccgagg cggtggacct gccgatgatc
    25741 gtctacaacg tgccgggccg gaccgtggcc gacctgcaga acgaaacggt gttgagactc
    25801 gcccaggtcc cgaacatcgt cggcatcaag gatgcgaccg ccaatctcga gcgcggctcg
    25861 gacctgctcc gccgcaagcc gcgcgacttc cgcgtctaca gcggcgacga cgccaccggg
    25921 ctcgccctga tgctgctcgg cggccacggc gtgatctcgg tcacggcgaa cgtcgcgccg
    25981 cggctcatgc acgagatgtg cgcggccgcg ctcgccggtg aggtcgtgaa agcgcgcgag
    26041 atcaaccacc ggctgctgcc gctgcatcgc aatcttttcc tcgaggcgaa tccgatcccg
    26101 gtcaagtggg cggtgcagca gatgggtctc atcaagggcg gcatccggct tccactgacg
    26161 ccgctgtccg ccgcctgcca tgagccggtg cgcgaggcga tgcgccacgc cggcatccgg
    26221 gccgacgtga aggaaggacg gtatgccgcc gcgtaaggtc gttctctgcc tgctggccgt
    26281 attgctgggc gccgtgttgg gtgcttgcag ctacatcggc ctcgaatcca agaagatcga
    26341 ttacaagtcg gcgggcaagc tgccgccgct cgaggtgccg ccggatctca cgcgcccgaa
    26401 cacggacgac cgctacgtcg tccccgacat caatcccaag agtactgcga cctattccga
    26461 gtacagccgc gagcgctcgg gccagcgtac cagcaccggc ggttcctcgg tgctgccgca
    26521 gttggacagc gcgcgcatcg agcgcgacgg cagtcagcgc tggctggtgg tgaagggggc
    26581 gcccgaccag gtttggccgg tggtcaagga tttctggcag gaggccggct tcatcgtcaa
    26641 tctcgaggtg cccgagtcgg gcgtgatgga gaccgactgg gcggagaacc gcgccaagct
    26701 tcccgacatc ggcctcatcc gcggattcct gagcaagatg ctcgacacga tctattcgac
    26761 cggtgagcgc gacaagttcc gcacccggct cgaacggggc agccagcccg gcaccactga
    26821 gatctacatc agccaccgcg gcatggagga agtctatacc tcctcgacct cttccagtga
    26881 tgcccagccg gatacccgct ggcagccgcg cccgcccgat cccaatctcg aggccgagat
    26941 gctgggccgg ttgatggtgc gcttcggcgt ggagaagtcc cgcgtccagg cgcagctcgc
    27001 cacgggcgcg agcgcgccgc agcgcgcgac gctgtccaaa ggcagcgacg gcgcgggcac
    27061 gctggcgctc gacgaccagt tcgaccgggc atggcggcgc gtcggcctcg cgctcgaccg
    27121 cgtcggattc acggtcgagg accgcgaccg ctccaagggg ctctatttcg tgcgctacat
    27181 tgacccgcag atcgacaaca agacggcgga caacaagggc tggttgtcgc gactgaagtt
    27241 ctggggcagc gacaagccca agcaggagca gtaccgcatc gaggtcaagg acgttgcccc
    27301 agggagccag gtcaaggtgc tcagcaagga cggcgcgcag gagaactccg ataccgccgg
    27361 caagattctt acgctgctct acgagcagct caaatgaatg aagcgtgatg ggtgacaggt
    27421 gataagtgat aagtgtgagt gaaaagcgcc tcacgcggtt cgtttttagc acctatcact
    27481 tatcacctat cacttatcac cgcccatgat gcgcttcgca tcactgggca gcggcagcca
    27541 gggcaacgcc ctcctcatcg aatccggccg tacccgcatc ctgctcgact gcggcttcgg
    27601 gctgaacgaa gtcacggcac ggctttcgcg gcttgggctc gctcccggcg acctcgacgc
    27661 gatcctcgtc acccacgagc acgaagatca tggcgggggt gtggcgaggc tctccgtccg
    27721 gtacgatatc ccggtttacc tcacgcgcgg cacgctctcg gccatgggcg ccgaaggccg
    27781 tgccattgcg aacaggatac tcatcgacgc ctatacgccc ttcgcaatcg acagtatcga
    27841 ggtaagaccc tacacggttc cgcatgacgc ccgcgagccg gtgcaattcg ttctctccga
    27901 cggggcagta aggctcggcg tattgacgga tacaggccat cccacgccgc acatcgcgca
    27961 ggccctgtcg ggagccgatg cactagtgct cgagtgcaac cacgacctcg acatgttgat
    28021 gaacggcccc tatccggcgc aactgaagag ccggatcgcg ggccggctgg ggcatctcgc
    28081 caacaaggca tcggcggaaa tcgtgcgagc catggattgc agccggctgc agcacgtgat
    28141 cgcggcgcat ctctcgcaga cgaacaacac gccggagctc gcgcgcgcag cgcttgccgg
    28201 cgcgctcaac tgtgcgccgg agtggatcgg cgtggcgacg caggacgaag gatttggctg
    28261 gcgggaaata cgttgatcag ccgtcgatga acgccgatac acaccgataa cttcaagaac
    28321 aaatagccac agagaacaca gagaaaaaca aaattgaacc gcagaggtac ggagagaaac
    28381 aagaacaata atggaaccac agatgaacac agataaacac ggataacgaa aagaacaagt
    28441 cgacccacga cccacgaatc acgatccacg gttcttatga accgccgagc acgccgagag
    28501 aagcaaaatc aaaatgaaag caaacagcgg ttcgctattt tccttcgtag taagtgcgat
    28561 ttaatctaac tgacgatttc cgattaggtc ggcttacggc ctaaagcggg cactgacgag
    28621 agatcgcttc gatggacgat cgatcccaag gagggggatc gatgctcggc cggcctattg
    28681 tggttgcccc ctgtggtctc cctgatcccg acgcgagctt gacatttaat tagccaattg
    28741 gcaaagtaac gaacatgctg cccgtttctg tcgtgaaggc cttggctagc gagcgtcgtc
    28801 tgcaaatcct cacatggctg aagcgaccga ccgtccattt cccgccgcag gttgatggcg
    28861 atctcgtccg tgacggagtc tgcggcgtgt tcatcgccaa gaagctgcgg gtgagccaac
    28921 cgacggccag cgaacacctc cggatcttga cacatgccgg gctcgtcaaa gccaagcgga
    28981 tcaagcaatg gacgttctac aagcgcgacg aaaggcggat cgcggaggtt aagcgggcat
    29041 ttcgctctgc ttaatggttg aacccacagg aggagtaagg cgtgcctaga gaagcctaca
    29101 cgaccgacaa gatcgcggca ccggtggggc cgttctcgcc cggggtgcgc gcaggagagt
    29161 tcgtgtacat cagcgggcag gtcgcgcagg atcccggcac cggacggctc gttgccggcg
    29221 atgtgggggt gcagaccgag cagatcttca ggaaccttgg accgcttctg cactcagcgg
    29281 gaaaaacgtt ttcagacgtc gtcaaggcca gcgtgttcct gaccgatatg cgcgatttcc
    29341 ctgcgatgaa cgcggtgtac gggcggcact tcgaggcacc ctacccggcg cgaacgaccg
    29401 tcggagttgc ggcccttcct ttgggtgccg cggtcgagat cgatctcgtg ctgcgatagg
    29461 gtctcatagg agccatcatg tcccatcgtt tcgtcatcgc tgacgtcttc acggaaattg
    29521 ccttcggcgg caaccagctc gccgtttttc cggacgggcg cggcctatcc gaccgcgcca
    29581 tgcaggcgct cgcgcgcgaa atcaattttg ccgagacaac tttcgttttg ccgccgaagg
    29641 atccccgaca cacgcgacgc gtgcgaattt acacacctaa gatggagctg ccgttcgccg
    29701 ggcatccgac agtcggcacg gctgcggtac tcacccgtct cggactggtc cagatttcgg
    29761 gcggcgcaac caccattgtt ctcgaggaag gagttgggcc tatcgcggtt gaaatccagg
    29821 ttcgtgggga agctaccttt gcccgtctgg tgacggaaaa agaagtcgag agtccatcca
    29881 cccgtcccgc gcgcaacgcg gttgcggcgg tactctcgct tcccgaggag gccatattgg
    29941 agacttggtt cgcaagcgtc ggcgtgccgt tctgcttcgt ccatttggcg gccaaggaga
    30001 ccgttgatcg cgctgtcctc gatcgcgtcg cgtggtctgc gaatttcgcg aacgcgtggt
    30061 caccgaattt gtatttcttc gccggcggac tcgttcccgg cagccgcctc tacgttcgca
    30121 tgttcgcccc tgccttgggg attgaagagg atccggccac cggctccggc gccgccgcgc
    30181 tggccggctg cttggccgcc cgatcgccag atcaggaggg gactttcaag tgggaaatcg
    30241 accagggagt cgcgatgggc cgtcccagcg tcatcgaggc ttcggccgag aaggtccacg
    30301 gccggacggt caaagtaaag gttggtgggg ccacggtgtt gatgggtgag ggaatgatga
    30361 ctgtcccgcc ggggtacgca tagcctaacc acccgctgca gtggtccgta gacggggcgg
    30421 ctgacgccga acgttgccac acttctgcag cgagcaatta tgcctgggag gacttgatga
    30481 acaacacggc tctcgtgcga tcgttctccc ggttgtccac tcccttgatc tgcgacgcgt
    30541 gtctgagact gggactaggt tacaggatgc caacccacgg gatccggccg cttcgtccgg
    30601 tgatgagaat cgcgggcaga gtgctcccgg cccgacacta tggcagcgtc gatgtgttcc
    30661 ttgaagccct cgagggtgca acagcgggag acattcttgt catcgacaac gggggccgtc
    30721 ttgacgaagc ctgcatagga gatctgatag ttcttgaggc caaaggtgcg agcctcggtg
    30781 gaatcgcggt gtggggtgta caccgcgact ccgccgaact cgcccttatc gacttccccg
    30841 tctacagcta tggcagctgt ccctcaggtc cacagcggct cgatgctcgg ccgtccgacg
    30901 ccctcgtttc agcctctttc ggagcctttc gggtgagtag ttccgatttc gtcgtcggtg
    30961 acgacgacgg gttagttttc gcgccgttgt cggaagctgg cagaatcttc gcgaccgccg
    31021 agagcatctg ggccactgag catgctcagg cggaggcgat tcgatccggc acaacgcttc
    31081 gagcacagct cagcttcgcc gagtaccttg aagcacggaa gcgagatcct tcgtatacgt
    31141 tgcgaatgca cctgaaacgt attggaggtg cgatagagga gtagaaggat cgggtggcta
    31201 accgaccttt taatccgttg gtgagcagtg gcggcgaagc caggcccgcg tatggtggat
    31261 gccggcccac acaagcgcca gcgcgagcgg gacaccccag aatattacgg ggtcgtaaaa
    31321 gaactcggaa aaccactcca gcattgggcg cagtcaagct gctggctggc gccagagccg
    31381 cgggaaaaac gccccgactc gcgcctggta gcgcaggtag tcctccccga agattctgac
    31441 caggcgccgc tcttcgtagt agcccccgat cagcatgtag gccgcgtaca tggcggtgta
    31501 gatcaggttg ttcacggagg gtccgctcgt gagcaggaaa aggagtcctc ccaccatcat
    31561 cgggtggcgc acccacccgt agactccctt caccactagc cgatgcgtcc cgaagagttc
    31621 cacggtcacg ggccggacgg gcattccaag gagtgcgggg agacctcgcc aagcctgctt
    31681 caagcccagg aactccaggt aatcgctctg taaaaacgcg acatagagga gggcaatgga
    31741 cccaagatgg agcacggtaa ccgcctgcca gagccactct ggataggcga tcagccagat
    31801 atcgttttcc ggatgccggg cccagtggaa cgccgccaca ccgtaataga gcgccacgaa
    31861 gctcaaggcg caatagacaa tgcgccaaaa gtgctcgacg aaaaacgggc tcgtccagcg
    31921 ggcaagcgca ttcttgaacg gctcctgcgc cccgatcgag tggaggaacg agaacaggcc
    31981 aaacgcgacg agcgagggtc cgtagtctcc caggtgcgag accgccgtta cgggaggcac
    32041 ggcggagcga gggtcgtctc agcctttctt ctgcaccgat tggtatgcct cgacggcctt
    32101 cttcaggtcc tcatactcct cgcacccccg tgggcaaagg ctttgaagct tcgcgagccg
    32161 ctcccgcgcc ttttgcggct ccccggtcat gagatacagc tcgcccagat actcgttggc
    32221 tccccgatgg tccgggttga tcgctagcgc tttctgatac gcagccagag attggtcgaa
    32281 ttgtttcagc ttccggtgac tgaagccaac gtagttccag gcgtccgcgt tgcgcggctc
    32341 ggcttggacc accttctgga gcagggtcaa ggcgcgttga tagtcacccg cctgcacggc
    32401 tttcaatccg agatcatagt cactgggttg ggcccttggc ggtgccgagg gtcgggatgt
    32461 gctgctcgag cccgccgcca tggcgggaga gacgagggcc agagtcaagg cgatagctaa
    32521 gagaagcttg cttatcatgg ggatccctcc tgtgattgcc atccacctgc tgtcaaggtg
    32581 aggtcggagt acggcaagtt aagaaaatct acggttggcc ttttcgttgt cagaaagagc
    32641 ctcggatgct aaaagcctca tctagtaaac agttactccc gccataacat tccctactgg
    32701 gcctgattaa tgaggacagg ccaatcatca taagccgcat aataaatcaa tctaagtccc
    32761 tacttcacga agacgtaacg taatgcccaa agctgataat gggctgagaa agcagttaac
    32821 cccactctca ctgtatggtt gtccatatag gacgtcagcc ggaatttaaa tacaactcgg
    32881 ccatctagtc tcatccccga aaggcaaaag agggttcttg gctcatccag tgcgctgaga
    32941 tgagctcgaa tcacgcagaa agctaccggc cgcgaatgtc cggaatgggt gattgcggaa
    33001 gctgctacaa atgaacacgg ccggctgtct ccagccggcc gctatgtttt cattcgtctt
    33061 gatttggttt ctctctgtgt gctctgcggt tcaattattg gttttgattt tcttggcgcg
    33121 cttggcggcc ttggcggttc attcttgatt ttattttttc ttgtttcgct tggcgccttg
    33181 gcggtgagag gttctacgcg gcgaagcgct tcagcgccgc ctcgtcgaac ttcagcccca
    33241 agccaggctt cttcggcacg gcgagctcgc ctttcaccgg caccggcact tcctcgaaaa
    33301 gcgggaacgt ccacggcatg tattccacgg tgaggccgtt cggcacggca gcgatgaggt
    33361 ggacgtggat ctcgggcaca aggtggctca ccaccggtag gttgtaggcc tccgccatgc
    33421 cggcgatctt gagccattgg gtgatgccgc ccgcgcgcat gaggtcgatc atgacgatgt
    33481 ccaccgagcg cgcttccagc atgtgacgga acggcaccgg cccgtacagg tattcgccgc
    33541 cgcagacggg tgttgagagc gcatccgcga cgcgggccag gcccggataa tcatcgtgcg
    33601 cggtcacgtc ttccagccag tagaggtgga cgtcttcgat gcgccggccg atttcgatcg
    33661 cctggtgcac gctccagcgc tggttgatgt cgcacatgag atcgatatcc gggccgatgc
    33721 tctcgcggac ccggcgcacg cgttccacct cgcgctcggg cgaggtatcg cccggcagcg
    33781 cgagttgtgt cttcatctgg cggaagccct tttcggcgag ccgtggcgcc gccttcacga
    33841 ggtgctccag ggaaaacgtg cgcatcagcg cgccgctcgc gtaggtcgga acgctctcgc
    33901 gcgatccgcc gatcagctgc cagagcggca ggttgagtgc cttgccccgg atgtcccaca
    33961 acgcgatgtc gatcgcggag agagcgagcg tgagaatgcc gccggggccg gagccggcgg
    34021 cggtggtgcg cagcttggcg gcgaccgcct cggtgcgaaa cgggtcttcg ccgatcgcga
    34081 ggttccccag cgcgtcaacc gcatgcttga gggcgccggt gagcgccgcg ccgaagaagg
    34141 tatagccgat gccttcgatc ccggcgtcgg tgccgaggcg caacgtgacc atatcgcgcg
    34201 tcgcaccggg cgttacagga ccgccagcga gcggctcgtc agcgggcagc cgcacgattt
    34261 gcgtctggat attcgtgatc ttcataaata ccgtggatcg tgagtcgtgg gtcgagtgtc
    34321 gagggacgag tgtcgaggga cgagtgtcga gcgtcgtgga tcgagagtcg aaaaagaaag
    34381 ccggcgcggg gccggctttc tttattgtgc cgccgagagt tacttcttct cttccttttt
    34441 catctcttct ttcttcatgt cgcccgcggg cgctgtcggg gctggcgcgc cggctgcaac
    34501 agtcgcctcc ggcttgggcg cctcagcggt cggcgaaggc ggcggaggcg gcgcgggagc
    34561 tgcttcggtc ggcttggcgg gcccggatgt tgtggttttg ggggcgggtt tttgcggttc
    34621 gccgcaggca cccagaccga ttgccacgag cgtggcgatg aggatcgagg aacgtttcat
    34681 gcttgttttc ccttggatgt atgaacagtc gataccaaaa acaacggccg ggttgcggcc
    34741 gatctgattt caacggcgga agaacttagc cgtcaggtgc gtattctagc tgaaaaaagc
    34801 gaatcgtgat tcgtgttcgg gggtcgtaag cttcatgtaa gctgagctca accagacggt
    34861 ttcctcgaat cacgattcac gatccacgac ccacggcttt tcagaggccg ctagtactgg
    34921 cggttaccgc gagcacggac acttcccaga tctcggcgaa gcggtcgagc agctgctgcg
    34981 cgccgaccag atcgttggtg ccctgggccg cgcgcaggta gcggtaatgg aagcggtgca
    35041 cgtaatggtt ggcgtcgaac accacgtacg gatcgtagac ttgcttcgcc gggctcagcg
    35101 tctcgtgaat cttgaccccc gcgccgaact gccgcacgag gtccaccagg cgcgggcact
    35161 cgcgctcgat cgtttcggcg tcgtgtacca cgatcagcag gcggttcgag cggctcgcga
    35221 gcaggaactg gcgcaacacc tcgtagcgct gcgccgtgtt ccaggcgcgt gacaggctca
    35281 tgtcgaaaac gcggatgacg cgctgtgtct gcggaatcag ctcgtcgatc acggcctcat
    35341 actcccgcat gccctcgaag cggcggtatt cggcgttcgg caggttgccc gccagctcca
    35401 cggctggagt tttttcgtca cccgtcactc gtcacccgtc agtcgtcacc gaaattaata
    35461 tacccggcgc gataccactg gtaaagcagt tgcgctgcct cgcgaacgag acgcaagggc
    35521 ggcggcagca tgcggtggtc ggcgagcctg gccaacagcc gggccgcggg tgcgccgacc
    35581 gggcaggcct cgccattgat gaaaacgata tcgccgcgaa acagcatccg tgtcggcagc
    35641 gagaggcgca cgccatggcg cgccgcgctt gcggagaacg tccgcttcgg cagggggcgc
    35701 agcgggcggg cgaagaccac gtgggccttg ggctcggtga gataacagcc cagaaaacgc
    35761 gccacgtcgc cgccgctcca gcgtatgctc tggaggaatc ctcgcacctt gcgcaccatg
    35821 tgatccccca gccgggccgg gtggcgcggt ggtttcaacc cctggtccgc gtagattccc
    35881 ggcagttgca gatgatcctg caggtaatcc aggaagcgtg cacccaattc ctgcgcgtcc
    35941 ggcgcgcgaa agccgaccga ataggtgatg cagggatctg ccgcaatccc gtcgtgcgcc
    36001 cagcgtggcg gcaaataaag catgtctccc gtgtgcaaca cccattcgcg cgccggccgg
    36061 aaacgcttca atatcctgag cggcgcgccg gcaaccagct cgagatcgca ttggcggctc
    36121 acgcgccagc gccgccggcc ttcgccctgc agcagaaaca cgtcgtagtt gtcgaagtga
    36181 ggaccgactc cgcccccggg aggcgcatag ctcaccatca ggtcgtcaag gcgggcgtac
    36241 gggataaacg cgaattcgtc gagtaacttc cgcgcggcgg gcagcgcgtg gttgacgccc
    36301 tgcaccagca gcgtccagcc tgatttcggc agtcgtccga gcatgcgctg gtcaaacggt
    36361 ccgtgccgca ccttccagcg ccggccttca cgctgcacca ggcgcgactc caggtcgtcg
    36421 cgctcggcga gcgcgagcag ggcgcggggc tgcacgatgt cagcgcactg tggcagggcg
    36481 ctccgcacca gcagcggctt cttctgccag taacggcgca ggaattcgcc cgccgaaagc
    36541 cccgcaaggc caagtttcgt catgcaaaac gatgataata agcagcctgc cggaccgcac
    36601 tgtccggcag tctgcttaat ttaacaccgc ctcaagaacg ggattggatc ggaaaaggcg
    36661 ccttgattca gaaggattat gccctgaccg ctgcgcaaat gagcgcgcaa cttcgtcttt
    36721 ggtgcgtaaa gcagtttttt taaagagtct gtcgagctga aggtcggcaa actcgttgcc
    36781 ggtagaattt ttttcaaaag cgcttgaccg cggccggtga tgttcacaaa atgaagcaag
    36841 agccaacatg ttgccggaaa ccctgcacac cgccaccatg ctgcaaccgg gacagccggc
    36901 gccgggcttc gacctgcccg acgccgacat gaacgtggtg agcctcgaga gcttcaggaa
    36961 ccgccgcaac gtcgtgctct atttctatcc caaggacgac acgccgggct gcaccctgga
    37021 ggcgatcgaa ttcagcgaga aggaggacga cttcgaggcg ctgaagacgg tggtggtggg
    37081 cgtgagcatg gacgactgcc tgacacacgg cgcgttccgg gacaagcacg gcctgtccgt
    37141 tcaactgctg gcggacgcgg aaggggaggt gtgccgccgc tatggggtgc tgcacgacaa
    37201 ggaagtggac ggcaggaaaa agacctgcat cgtgcgctcg accttcatca tcgaccgcaa
    37261 gggcgtgctc aagcacgcgc tttacggggt gaacccgcga ggtcacgcgc aggagatcct
    37321 cggcctgctc aaggggatcg gcaggtgcaa atagcgaaca acaccgtcgt ttcgctgacc
    37381 tacgagctac tggacaccga cggcaagacg atcgagaaga cggacgcgcc gatcgaatac
    37441 ctgcacggcg gccacgacgg catcttcccg ctggtggaga aggcgcttgc cgggaagagc
    37501 acgggcgaca gctgccgggt ccggctcgag cccgacgatg cattcggaga gtacgacgcg
    37561 gagctggtgc gtctcgagcc gcgcagcaag tttcccggca acatcgcggt cggcatgcag
    37621 ttcgagggac gcggcgcgga gtcgggcgcg acgctgatct acacggtgac cgacgtcgcc
    37681 gaggacaagg tggtggtgga cggcaatcat ccacttgcag gccggacgct ggaatttgcc
    37741 tgcaccgtga ccgccgtgcg cgcggcgacc ggggaggaga ttacccacgg ccacgtgcat
    37801 ggtgatggtg gccaccatca ctgatcgtaa gcggtaagcc gtaagcggta tgcgggaaaa
    37861 cgttcttacg gctcactgct cacggctccc cgcttacggg cgttagctcc ctggcgtcac
    37921 ctcgaaccta acgcgtctat cttcttcacg gacgcgtatc cgcacccagt tcatcgaggg
    37981 gctgccgaac acctcgacgc gggtgaagtt ggcgagtgtc gcgccggtcg cggaatcgcg
    38041 cagcggccgg tccacgcggt aacggtgtgt gtcgccgtgc acgagcaaca cctctccgtg
    38101 aaactcgcga gtttcgctcg cgagcgcagc cagcagctcg cgatagccga agtaacggcc
    38161 ggtcggtgag gcccaaggat tgccctgcat caggatcacc accgcgcgca gcccgttggc
    38221 acgcgcgaga ctgtacgact gctcaagcca cttgccgccg gtcgtgctgc ggcggcggaa
    38281 ttcctccggc atgaggcgcg
//
LOCUS       DBNGWZ010000044         4855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_255734, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000044 DBNGWZ010000000
VERSION     DBNGWZ010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_255734"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="mobB"
                     /locus_tag="AC16CX_01405"
     CDS             complement(<1..255)
                     /gene="mobB"
                     /locus_tag="AC16CX_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR3011198.1"
                     /translation="MKIFGFAGWSGSGKTTLIERLIPLFIARGLKVSLIKHAHHSFDV
                     DQPGKDSYRHRHAGCTEVLVTSSRRWALVHELRGAPEPGFA"
     gene            453..1175
                     /locus_tag="AC16CX_01410"
     CDS             453..1175
                     /locus_tag="AC16CX_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3011199.1"
                     /translation="MAFFAVATLYTTVGHAGASGYLATMALVGLSPEAMRPTALTLNI
                     LVAAFTVYRFRRARHFHWSGLWPFLLGSVPFAAAGGIQNLPRGAYYAAMGVVLLLAAL
                     YLVWRALGSRVVMEEGIVRVRKLPAVFIGCVIGFISGLIGVGGGIFLSPILLVLNWAG
                     PKTTAGISAPFIFVNSAVALVAGSLTVQTLPRELPLLAAAALGGAFVGTWLGLQRLKQ
                     KGLLMTLAIVMSLAGLKLLLTA"
     gene            complement(1291..1656)
                     /locus_tag="AC16CX_01415"
     CDS             complement(1291..1656)
                     /locus_tag="AC16CX_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3011200.1"
                     /translation="MARYPGLTLRVLAAKQPAMGPGRAALLSHIARTGSITSAAQAMG
                     MSYRRAWQLVEAMNESFNEPVVTTAVGGRRGGGAKVTALGKRLVRLYHEMETKASSAI
                     AADLKKFSRSLRKPRGTPR"
     gene            complement(1677..2486)
                     /locus_tag="AC16CX_01420"
     CDS             complement(1677..2486)
                     /locus_tag="AC16CX_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011201.1"
                     /translation="MKLEARHLDFGYRDKRVGGDVTLSVEAGEVLCLLGPNGSGKTTL
                     FKTLLGLLPTQGGQVLVDGTDLRQKRRDEVARLVSYVPQAHAAFFPFTVREVVLMGRT
                     AHLGVFSSPSRRDLDIAHAAIERMRLAHLAGSIYTRISGGERQLALIARALAQDARII
                     VMDEPTANLDFGNQVRVLRHIQSLAQSGIGVLLSTHDPDQVFLCADRVAMLHEGRLAH
                     FGAPVEVVTSDSLKQIYGVEVIVTRVKMNGEGERLVCIPTGSLAVERQASR"
     gene            complement(2689..3705)
                     /locus_tag="AC16CX_01425"
     CDS             complement(2689..3705)
                     /locus_tag="AC16CX_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR3011202.1"
                     /translation="MSFARVSLAVVAACAALAGLVVVAFSVGRFPVAAGDLASLLWAH
                     LTGSEHGLDPTIETVVMKIRGPRVMAAVMIGAALAAAGAAYQNMFRNPLVSPDILGVS
                     TGAAVGAVLGIFLSLNVLAIQSLAFAFGLGAVGLVYVTANAVRGHDPLLVLVLAGVVV
                     GSLLGACVALMKYLADPYNQLPAITFWLLGSLASATPGDVWSTLPLMLVGLAPMWLLR
                     WRINLLSLDDEEARAMGVETGRIRLAVIAAATLMTSASVAISGVIGWIGLVIPHFARM
                     LVGPDFSRLLPTSVLLGAAFLLGVDTLARTVARIEIPLGVLTAFVGTPLFLWQLAAAR
                     RGWQ"
     gene            complement(3788..>4855)
                     /locus_tag="AC16CX_01430"
     CDS             complement(3788..>4855)
                     /locus_tag="AC16CX_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HIR3011203.1"
                     /translation="PFILAYITIVGVEPAGMLLAFGIAYIAAGAVFRTPFPVQPMKAI
                     GAVAITGAAQTAVVTPGAVAVAALATGLIWLALGAAGAADRVARWVGRPVVIGIILGL
                     GLAFMLDGTRMMAKGIWLAAPLLIATILLLSNRALLAMILILAVGAAWAVLADSAVAA
                     ALGRVEAGFKLPSWPFHGLTWEAFFVGVVLLALPQVPLTLGNAIIAPVEFNNREFPGK
                     PATERQVAISTGVMNTLGSLIGAVPMCHGAGGMAAQVSFGARTGGAPIILGILLVTLA
                     LLFSESVATLLRLFPQPALGVMLFLAGVQLALGSCDFSKDKGERFVTLGTAALAVWNV
                     GVAFVFGITVLWIVRRGWLRL"
BASE COUNT          918 a         1647 c         1512 g          778 t
ORIGIN      
        1 cgcgaagccc ggttccggcg caccgcgcag ctcgtgcacc agcgcccagc gtcgcgacga
       61 ggtcaccagc acttccgtgc agcccgcgtg ccggtggcga taagaatcct tgcccggctg
      121 gtccacgtcg aagctgtggt gcgcgtgctt gatcagcgac actttgagcc cgcgcgcgat
      181 gaacaacggg atcagccgct cgatgagcgt agtcttcccg ctgccggacc agcctgcgaa
      241 tccaaatatc ttcatgctag gaatttgtcg gacatcgagc cgtcaaattc cttaggcgca
      301 atcatagcgg accccctcgc aaaaatcgaa ttcaatgtcc gtctgccggt caaggattgc
      361 ggccgaggca accgttattc ccattaagga acaacgatga tacctgtgct atggtagtgg
      421 acatggaatt atccgaggcc gcaattcttt gtgtggcgtt cttcgccgtg gcgacgctct
      481 atacgacggt cggccatgcc ggcgcatcgg gctacctggc tacgatggcg ctggtcgggc
      541 tgtcgccgga ggccatgcgg ccgacggcgc tgacactcaa catactggtg gccgcattca
      601 cggtgtatcg cttccgccgc gcgcgccatt tccactggtc gggattgtgg ccgtttttgc
      661 tcggctcggt gccgttcgcc gcggcgggag gcatccagaa ccttccgcgc ggcgcgtatt
      721 acgcagcgat gggcgtggtg ctgctgcttg cggcgctcta cctcgtctgg cgcgcgctcg
      781 gctcgcgtgt cgtcatggag gaaggcatcg tgcgggtgag gaagctcccg gctgttttca
      841 tcgggtgcgt gatcggcttc atttcgggcc tgatcggggt cggcggcggc atcttcctca
      901 gccccatcct gctggtgctc aactgggccg gcccgaagac gactgcgggg atttccgcgc
      961 cgttcatctt cgtgaactcg gccgtggcgc tcgtcgccgg gtcgttgacc gtgcagacgc
     1021 tgccgcgcga gctgccgctg cttgccgccg cggccctggg cggggctttc gtcggcacct
     1081 ggctgggcct gcagcgcctg aagcaaaagg ggttgctcat gaccctcgcc atcgtgatgt
     1141 cgctggcggg gctgaagctc ctcctgacgg cgtagccccg cgcgagcaga ccagagcgtt
     1201 tctcaaaaaa cgtctagaaa ccctggaacc gccgatcaac gccgatccac gtttaactgc
     1261 ggtttcaggc agcttttcga gacagcttca tcaccgcgga gtgccgcgcg gcttgcgcag
     1321 gctcctcgag aactttttca ggtcggccgc aatcgcgctg gaggccttgg tctccatctc
     1381 gtggtaaagc ctcaccaggc gtttgccaag ggccgtcact ttcgcccccc ctccccgcct
     1441 gcccccgacc gctgttgtga cgacgggctc gttgaagctt tcgttcatgg cctcgaccag
     1501 ttgccaggcg cggcgatacg acatgcccat tgcctgcgcg gcggacgtga tcgagcccgt
     1561 tctcgcgatg tgcgagagca gcgccgccct gcccgggccc atggccggct gtttggcggc
     1621 gagcacgcgg agcgtcaatc caggatagcg ggccatcgtc cgattctaac cgctgttcag
     1681 cgtgaagctt gcctttccac ggccagggag ccggtgggta tgcagaccag ccgttctccc
     1741 tccccattca tcttcacgcg cgtgacgatc acctccacgc catagatctg cttcaggctg
     1801 tccgatgtaa cgacctcgac cggcgcgccg aaatgcgcga ggcgcccctc gtgcagcatg
     1861 gctacgcggt cagcgcacag gaaaacctgg tcgggatcgt gcgtggaaag caatacgccg
     1921 atgccggact gcgccagcga ctggatatgc cgcaggacgc gcacctggtt gccgaaatcc
     1981 aggttggcgg tcggctcatc catgacgatg atgcgcgcgt cctgggcgag cgcccgcgcg
     2041 atcagcgcaa gctgacgctc tccgcccgag atgcgcgtgt aaatcgaacc agctaggtgc
     2101 gcgaggcgca tgcgctcaat ggcagcgtgc gcaatgtcga ggtcgcggcg cgacggcgac
     2161 gaaaagacgc caaggtgggc ggtgcgcccc atcagcacca cctcccgcac cgtgaacgga
     2221 aagaacgccg cgtgcgcctg cgggacgtag ctcaccagcc gcgcgacctc gtccctccgc
     2281 ttctgcctca ggtccgtgcc atcaaccagc acctgcccgc cctgcgtcgg cagcaggccg
     2341 agcagcgtct tgaacagcgt ggtcttaccg ctgccgttgg ggccgagcag gcacagcacc
     2401 tcccctgcct cgacgctgag cgtgacatcg ccgccgacac gcttgtcgcg gtagccgaaa
     2461 tccaggtgtc tcgcttccag tttcaaccgt tcaaccccaa tacattaacc gcagataaac
     2521 acagatcaaa aagcaacaaa tagacagtat tcaaaaaatg tacaggatta actcaaatca
     2581 agaagggccg tcacattctt gtttgaatct tgtcaatcct gttaatcccg tccattctgg
     2641 tgttgatctt atccgcgttc atctgcgtgt atctgcggtt gcattgcttt actgccagcc
     2701 gcgcctcgcg gcggcgagct gccacagaaa gagcggcgtt ccgacgaacg cggtcagcac
     2761 gccgagcggg atttcaatgc gggcaacggt gcgcgccaac gtatccacgc ccagcaggaa
     2821 cgcggcgccg agcagcaccg aagtcggcag caagcgcgag aaatccggcc cgaccagcat
     2881 ccgcgcgaag tgaggaatga ccagaccgat ccagccgatc acgcccgaga tcgccaccga
     2941 cgccgatgtc atcaaggtcg ccgccgcgat caccgccagg cggatgcggc cggtctcgac
     3001 tcccatcgcc cgcgcctcct cgtcgtccag cgagagcaga ttgatccgcc agcgcagcag
     3061 ccacatcgga gcgaggccga cgagcatcag cggcaacgtg gaccagacgt cgcccggcgt
     3121 cgccgaggcg aggctgccga gcagccagaa ggtgatcgcc ggcagctggt tgtagggatc
     3181 cgccaggtac ttcatgagtg cgacgcaggc gcccaacagc gagcccacca ccacgccagc
     3241 cagcaccagc accagcaggg gatcgtggcc ccgtaccgcg ttggccgtca cgtaaaccag
     3301 gccgaccgcg cccagcccga acgcaaaagc gaggctttgg atcgcgagca cgttgagcga
     3361 aagaaagatg cccagcaccg cgcccaccgc cgcgccggtg gacaccccca ggatatccgg
     3421 ggaaaccagc gggttgcgga acatgttctg ataggccgcc cccgccgcgg ccagcgccgc
     3481 gccgatcatg acggccgcca tgacgcgggg gccgcggatc ttcataacga cagtctcgat
     3541 ggtgggatcg agaccatgct cggagccggt caggtgcgcc cacaacaggc tcgcgagatc
     3601 acccgccgcc accggaaagc gcccgaccga aaaggcgacg accaccagcc ccgccagcgc
     3661 cgcgcaagcg gccactaccg ccaatgaaac gcgcgcgaag ctcatgccag cgcgccggct
     3721 tggcgcggca acggatcgag ctgcgaattc ggcgacggct tgcgtccggc agtgcgcgcg
     3781 atatcgctca tagacgcaac cagccccgcc gcacgatcca cagcaccgtg atcccgaaca
     3841 cgaacgcgac gccgacgttc caaactgcca gcgccgcagt gcccagcgtc acgaagcgct
     3901 cgcctttatc cttggagaaa tcgcaggaac ccagcgcaag ctggacaccg gccaggaaca
     3961 gcatcacgcc cagcgccggc tgcgggaaga gccgcagcag agtcgcgaca gattcgctga
     4021 atagcagcgc cagcgtgaca agcaatatgc ccaggatgat gggcgcgccc ccggtcctcg
     4081 cgccgaagct cacctgagct gccatgccgc ccgcgccgtg gcacatcggc actgccccga
     4141 tcaggctgcc cagcgtgttc atgacgccgg tcgagatggc gacctggcgt tcggtggccg
     4201 gcttgccggg aaattcccgg ttattgaatt ccaccggcgc gatgatcgcg ttgccgagcg
     4261 tgagcggaac ttgcggcagc gccagcagca ctacgccgac gaagaaggcc tcccaggtca
     4321 gcccgtgaaa cggccaggaa ggcagcttga accctgcctc cacccgcccc agcgccgcgg
     4381 caacggccga atcggccaga accgcccacg ccgcgcccac ggcgagaatc aggatcatgg
     4441 cgagtaatgc gcggttggac agcaggagga tcgtggcgat cagcagcggc gcagccagcc
     4501 agattccctt ggccatcatg cgcgttccat ccagcatgaa ggcgaggccc aggccgagga
     4561 tgatgccgat gacgaccggc cgcccgaccc agcgcgccac gcggtcggca gcgccggctg
     4621 ctccaagcgc aagccagatg agcccggtgg ccagcgcggc caccgccact gccccgggcg
     4681 tgacgacggc ggtctgggca gcgccggtaa tggcaaccgc accgatggcc ttcatcggct
     4741 gcaccgggaa cggcgtgcgg aaaaccgcac cggcggcaat atacgcgatt ccaaacgcca
     4801 gcagcatgcc ggcgggctcg acgccaacga tggtgatata ggcgagtatg aacgg
//
LOCUS       DBNGWZ010000045         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3152672, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000045 DBNGWZ010000000
VERSION     DBNGWZ010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3152672"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16CX_01435"
     CDS             complement(<1..583)
                     /locus_tag="AC16CX_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011204.1"
                     /translation="MSDDPAIAVRGLDVTYGAIRAVQGVDIDAPRGQITALIGSNGAG
                     KSSTLNAIAGLVPVAGGSVEIGGVDATRLPAHVRVVERGVVLIPEARAAFLTMTVREN
                     LGLGIRVGREREARGQKGAFTLEDAFRLFPVLEERADKRAQYLSGGELKMLAIARSLL
                     MHPRVLLIDEPSMGLAPLLVRQIFAVLRGILREH"
     gene            complement(576..1358)
                     /locus_tag="AC16CX_01440"
     CDS             complement(576..1358)
                     /locus_tag="AC16CX_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011205.1"
                     /translation="MSALPSGTPAPAPAGAGALLTIEGLTQRFGGVAALTDVSFSVDS
                     HTVQALIGPNGAGKTTLFNAITGFLRPDSGRVYFKGRRIDGMAPQRIARLGMVRSFQS
                     IRMFKGLTVYESLLVSRPRERRWSVRAAQAKVGRILERMRLAEVADRVCSDLPLLAQR
                     RVEVARALMTDPTMILLDEPSAGATVPERHALVELIAGLKAAGTTVMVIEHNVPFVMQ
                     VSDRLAVLDFGKKVAEGTPAEIAGNPIVREIYLGSNKEQGGV"
     gene            complement(1371..2483)
                     /locus_tag="AC16CX_01445"
     CDS             complement(1371..2483)
                     /locus_tag="AC16CX_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011206.1"
                     /translation="MTALILAVVGGSIGGLAATGTVKVGVIEMLTGGSAFYGNAVLEG
                     IKVAEAEINAAGGINGNNLELVILDNASDNAQAAALTAQLASDKSIPVGITPTYQPNF
                     NAACARANADGFPMVSAQSGPPDPKNNPKGWCWTMTTDPVGQATYTFQTLKAKKGWQR
                     FVMVYDQDNGYVAFQRPNIRESAKAAGVDLQEVGVAAGTSDFGPQITRARNANADAVF
                     PFFTIEDAARFMKQARERGLATPWFDPVSQLTSKRLPQLSGGAATGLLASTPQSADDI
                     PSFKAFLSSYAKLNGKELDDPTYTGFGYDALRLIAMAMKDAGTTTDRAAIKAQINKYK
                     SPCFSICFTGRQDIGSFLAGKFYLVTLSADKGFVRF"
BASE COUNT          443 a          902 c          848 g          462 t
ORIGIN      
        1 cgtgctcccg caggatcccg cgcagcacgg cgaagatctg ccgcacgagc agaggggcga
       61 ggcccatcga cggctcgtcg atcaagagca cccgcgggtg catgaggagt gaccgggcga
      121 tggcaagcat cttcagctcg ccgcccgaga ggtactgcgc ccgcttgtcg gcgcgttcct
      181 cgaggacggg gaaaaggcgg aacgcgtcct ccagcgtgaa ggcacccttc tgcccccgcg
      241 cctcgcgctc gcgcccgacc cggattccca gcccaaggtt ctcccgcaca gtcatcgtca
      301 ggaacgcggc ccgcgcctcc gggatcagca cgacgccgcg ctcaactacc cgcacgtgcg
      361 ccggcaggcg ggtcgcgtcc accccaccga tctcaacgct gcccccggcg acgggcacta
      421 ggcccgcgat cgcgttcagg gtggacgact ttccagctcc gttcgagccg atcagtgcgg
      481 tgatctggcc gcgcggagca tctatgtcga caccctgaac cgcccggatc gcgccatagg
      541 tcacgtcgag accgcggaca gcgatcgcgg ggtcgtcaga caccgccctg ctccttgttt
      601 gaaccgaggt agatctcccg cacgatcggg tttccggcga tctcggccgg cgtgccctca
      661 gcaaccttct tgccgaagtc gagcacggcc aggcgatcgc tgacctgcat cacgaacggg
      721 acgttatgct cgatcaccat cacggtcgtt ccggcggcct tcagcccggc aatcaactcc
      781 acgagcgcgt ggcgctccgg aacggtcgct ccggcgctcg gctcgtccag caggatcatc
      841 gtcgggtcgg tcatcagcgc gcgggccact tcgacgcgcc gctgcgcaag aagcggcagg
      901 tcgctgcaga cgcgatcagc gacttcggcc agccgcatgc gctcgagtat gcgcccgacc
      961 ttcgcctgcg cggcgcggac tgaccagcgg cgctcgcgcg gccgtgagac gaggagggac
     1021 tcgtacacag tgagcccctt gaacatgcgg atgctctgga agctccgcac catcccgagc
     1081 cgcgcgatgc gctgcggggc catgccgtcg atgcgccggc ccttgaagta cacgcggccg
     1141 ctgtcgggcc gcaggaagcc cgtgatcgcg ttgaacagcg ttgtcttgcc ggcgccgttg
     1201 ggcccgatca gcgcctgaac ggtgtgggag tcaaccgaaa acgagacatc ggtgagcgcc
     1261 gcgacaccgc cgaatcgctg ggtgagtcct tcaatggtaa gaagcgcgcc tgccccggcc
     1321 ggagccgggg caggagtgcc gctgggcaac gcgctcatca tgcgcctgct ttagaaacga
     1381 acgaaaccct tgtccgccga cagcgtgacg agatagaatt tcccggcgag gaacgaaccg
     1441 atgtcctgcc ggcccgtgaa gcagatcgag aagcaggggc tcttgtactt gttgatctgt
     1501 gccttgatgg ccgcgcggtc ggtcgttgtg ccggcgtcct tcatggccat ggcgatcagg
     1561 cgcagcgcgt cgtagccgaa ccccgtgtac gttggatcgt cgagctcctt gccgttcagc
     1621 ttcgcatagg agctcaggaa cgccttgaac gacgggatat cgtctgccga ctgcggcgtg
     1681 gatgcgagga ggccggtcgc cgcgccgccc gacagctgcg gaagccgctt ggacgtgagc
     1741 tgactgacgg gatcgaacca cggcgtcgcg aggcctcgct cccgcgcctg cttcatgaac
     1801 cgcgcggcgt cctcgatcgt gaagaacggg aagaccgcgt cggcgttggc attcctcgcc
     1861 cgggtgatct gcgggccgaa gtcactcgtc ccggcggcaa cgccgacctc ctgcaggtcc
     1921 actcccgcag ccttcgccga ttcgcggata ttcggacgct ggaaggccac gtagccgttg
     1981 tcctggtcgt acaccatcac gaacctctgc caacccttct tcgccttgag cgtctggaac
     2041 gtgtacgtcg cctggccgac cgggtcggtg gtcatcgtcc agcaccagcc cttaggattg
     2101 ttcttcgggt cgggcgggcc ggattgagcc gacaccatcg ggaagccgtc ggcattcgcg
     2161 cgagcgcacg cggcgttgaa gttgggctga tacgttggcg taattccgac cgggatgctc
     2221 ttgtcgcttg caagctgcgc ggtgagcgcg gccgcctgcg cgttgtcgga cgcattgtcc
     2281 aggatcacga gctcgaggtt atttccgttg atcccgccgg ccgcgttgat ctcagcctcg
     2341 gcgaccttga tcccctcgag cacggcgttg ccgtagaatg cgctgccgcc cgtcagcatc
     2401 tcgatgacac caaccttgac ggtgccagtt gccgcaaggc caccgatcga gccgccgacc
     2461 accgcgagaa tgagtgcggt cacgatgaca ccggcgatta cgcaggtcct cttcacaagt
     2521 ttcaaccccc tccacttcaa gataagtccg gctctactcg gatcgcagag tttccatcct
     2581 gttgggttgt gccagcgttc ggtgcgatat tcgggaatcc cttccccaac gcccgtacgc
     2641 ttgcgcgatc cagga
//
LOCUS       DBNGWZ010000046         3579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2900766, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000046 DBNGWZ010000000
VERSION     DBNGWZ010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2900766"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..356)
                     /locus_tag="AC16CX_01450"
     CDS             complement(204..356)
                     /locus_tag="AC16CX_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011207.1"
                     /translation="MIGNSIRPHNPPHRSRLHVASAGTLAAVVVIGGFPQTATWLPEV
                     GLAIGK"
     gene            385..2529
                     /locus_tag="AC16CX_01455"
     CDS             385..2529
                     /locus_tag="AC16CX_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011208.1"
                     /translation="MHTARIPSIGAAAIAAVLITTPIGAQQAPAVSIGKVDIGGVVTG
                     PKGPEAGVWVITETTELPTKYAKIVVTDDRGRYLIPDLPPNVNYQVWVRGYGLVDSPK
                     LRAKPGQILNHSAVPAPTEAAAAHYYPAVYWYSMLKMPPAKDFGGTTNIPKEVTQEIW
                     RRRMNNTDCIGCHTLGQESTRTIPAALGEFLSGTEAWARRVASGHTGSFMVERLAKQL
                     GGVPFQYYGDWTDRIAKGELPKQKPPRPQGVERNVVITSWEWGTEKTFLHDLISSDRR
                     DPTVNAYGPLYGSAEYSSDDIPILDPKTHTVTNFRMSVLNDSVPESFGPPFHATALDK
                     PFAPSPYWRMEKIWTQKANNHNGMFDRKGRVWFAANVQGIENPAFCKKGSDHPSAKAF
                     PMDRSQRQVTMLDPKTMKYTLIQTCFGTHHPQFGYDPDDTLWFSGSGPVAGWLNTKVF
                     DETGDAVKAQGWAPWVLDTNGNGKVDEYTEPGKPAESGKDMRIAGSGPYAVMPHPTDG
                     SVWYTFNVFVGTAGFLRFDPRTRLSEFYALPPGYVGVRGGDIDKNGVLWGSGSNGGFI
                     SFDRRKCKGPLNGPTATGNHCPEGFAYHRFPGPGHEGFENNSAEASYYTWVDHHNTLG
                     LGENIPIATGNLNGGFIALKDGKMIMLSIPYPMGFFAKGLDGRIDDPNAGWKGRGLWS
                     TSGDRTPWLMEGGKGSKPRAVHIQVRPDPLAR"
     gene            complement(2537..>3579)
                     /locus_tag="AC16CX_01460"
     CDS             complement(2537..>3579)
                     /locus_tag="AC16CX_01460"
                     /inference="COORDINATES: protein motif:HMM:NF005566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3011209.1"
                     /translation="MTVPVLLYRLLDGAGSLLWEEPVAYRFVSWGSLYRALLGEFGRE
                     RYLQGEALVAFGQDGQGVDLHFASGRQLRYDLLVLADGVLSTGRQRLFPGVEPVYAGY
                     VAWRGTVAERDIPADVLARIDDTITYALIPNSHILVYPIPGSGGRVDRGHRRWNFVWY
                     RNVEKGAALDELMTDRDGFPHTVSLRPGRVQSRYVRELKELAAAMLPHEIAFIVTATE
                     DPFIQSVTDVESPAMPVGRVCLLGDAAFAARPHAAAGTAKAAENAWTLAEAIEGSDGD
                     LPGALQAWSAHQTALGSSLIARTRDIGEGSQFRCDWRPGDPRLRFGLYEAGDSSIWTR
                     RPRPLFPHRKKV"
BASE COUNT          684 a         1190 c         1159 g          546 t
ORIGIN      
        1 actgctgagc ggtggcagtc gcgcccagaa gcaacgcagc aaggccggct gctatcagca
       61 ctcgcggcac atacataaaa actccttcgc ttcggagaaa aaggcagaca cgggacggct
      121 ggacccatgg ccccgctgtc cgcggcctgg gcctggaagt acagcatacc gccttcaggc
      181 cgtcgcgtcg cccgattcca cgatcacttg ccgatggcca gcccgacctc gggcagccag
      241 gtcgcggtct gcgggaaccc gccgatcacc acgaccgcgg cgagcgttcc agcgcttgcc
      301 acgtgcaggc gggatctgtg gggggggttg tgcggccgga tcgaatttcc tatcattttc
      361 cacttcgtcc aagaggagga gatcatgcat acggcacgca ttccgagcat tggcgcggcc
      421 gcgatcgcgg cggtcttgat tacaacgccc atcggcgcgc agcaggcgcc cgcggtttcg
      481 atcggcaaag ttgacatcgg cggagtcgtc accggcccga agggaccgga agccggcgtg
      541 tgggtgatca cggagacgac cgagctgccc acgaaatacg ccaagatcgt cgtgaccgac
      601 gatcgggggc gctatctcat ccccgatctg ccgccgaacg tgaactacca ggtctgggtg
      661 cgcggctacg ggctcgtcga ttcgcccaag ctgcgcgcca agcccggcca gatcctcaac
      721 cacagtgcgg tgcccgcgcc cactgaggct gcggcggcgc actactaccc ggcggtctac
      781 tggtattcga tgctgaagat gccgcccgcg aaggacttcg gcggcacgac caacattccc
      841 aaggaagtca cccaggagat ctggcgccgg cggatgaaca atactgactg catcggctgc
      901 cacacgctcg gccaggaatc gacgcgcacg attccggccg cgctcggcga attcttgtcg
      961 ggcacggaag cctgggcgcg ccgcgtcgcc tccggccaca ccggatcgtt catggtcgag
     1021 cggctcgcca agcagctcgg cggcgtgccg ttccagtact acggcgactg gactgaccgc
     1081 atcgcgaaag gcgagctgcc caagcagaag ccgccgcggc cgcagggcgt cgagcgcaat
     1141 gtcgtgatca cgtcatggga gtggggcacg gagaagacgt tcctccacga cctgatttcc
     1201 tcggatcggc gcgatcccac ggtcaacgcc tacgggccgc tctacggctc ggccgaatac
     1261 tcgagcgacg acatcccgat tctcgatccg aagacgcaca cggtgaccaa cttcaggatg
     1321 tctgtcctca atgacagcgt ccccgagtcg ttcgggccgc cgttccacgc cacggccctt
     1381 gacaagccgt tcgcgccgtc gccgtactgg agaatggaga agatctggac gcagaaggcg
     1441 aacaaccaca acggcatgtt cgaccgcaag ggccgggtgt ggtttgccgc taacgtccag
     1501 ggcattgaga accccgcgtt ctgcaagaag ggctcggatc acccgtcggc aaaggcgttt
     1561 ccgatggacc ggtcgcagcg ccaggtaacg atgctcgatc cgaagacgat gaagtacacc
     1621 ctcatccaga cctgcttcgg cacccaccac ccgcagttcg gctacgaccc ggacgatacg
     1681 ctgtggtttt ccggctccgg tcccgtggcg gggtggctga ataccaaggt gttcgacgag
     1741 accggcgacg cggtcaaggc gcagggctgg gcgccgtggg tgcttgacac caacggcaat
     1801 ggcaaagtgg acgagtacac cgagcccggc aagccggctg aaagcggcaa ggacatgcgc
     1861 atcgcgggct ccggtcccta cgccgtgatg ccgcacccga ccgacggctc ggtgtggtac
     1921 acgttcaacg tgttcgtcgg caccgccggc ttcctgcgct tcgatcccag gaccaggctc
     1981 tccgagttct acgcgcttcc gccgggctat gtcggcgtgc gcgggggcga catcgacaag
     2041 aacggcgtcc tctggggctc gggctcgaac ggcggcttca tcagcttcga ccgcaggaag
     2101 tgcaagggac cgctcaacgg gccgaccgcg accggcaacc attgcccgga aggctttgcc
     2161 taccaccgct tccccgggcc aggacacgag ggcttcgaga ataacagcgc cgaggcgagc
     2221 tactacacct gggtcgacca ccacaacacc ctcggccttg gcgagaacat tccgatcgcc
     2281 accggcaacc tgaacggcgg cttcatcgcg ctcaaggacg ggaagatgat catgctgagc
     2341 atcccgtacc cgatgggctt tttcgccaag gggctcgacg gacgcatcga cgatccgaat
     2401 gcgggctgga aaggccgcgg gctgtggagc acgagcggcg accgcacgcc gtggctgatg
     2461 gagggcggca agggctcgaa gccccgcgcc gtgcacatcc aggtaaggcc cgacccgctc
     2521 gcgcgctgaa cgagcgttac acctttttcc gatgcgggaa gagcgggcgg ggccggcgcg
     2581 tccagatgga ggaatcaccc gcctcgtaga ggccgaatcg caggcgggga tcacccgggc
     2641 gccaatcgca gcgaaactgc gagccttccc cgatatcccg cgtccgggca atgaggctcg
     2701 agcctagcgc cgtctgatgc gcggaccacg cctgcagcgc tcccggcaaa tcgccatcgg
     2761 atccctcgat cgcctcggcg agagtccatg cgttctcggc ggctttcgcc gtgccggccg
     2821 ccgcatgcgg gcgggcagcg aacgcagcgt ccccgaggag gcacacccgg ccgaccggca
     2881 tggcgggcga ttcgacatcc gtgaccgact ggatgaatgg atcctccgtg gccgtgacga
     2941 tgaaggcaat ctcgtgcggc agcattgctg cagcgagttc cttcagttcg cgcacgtatc
     3001 ggctttgcac gcggcccgga cgcagcgaaa cggtatgcgg gaatccgtcg cggtcggtca
     3061 tgagctcgtc gagagcggcg cccttctcga cgttccgata ccagacgaag ttccagcgtc
     3121 ggtggccccg gtcgacacgg ccaccggaac cggggatggg atagacgaga atgtggctgt
     3181 tcggaatgag cgcgtaagta atcgtatcgt cgattcgtgc aagaacgtca gcggggatgt
     3241 cccgctccgc gaccgtgccc cgccacgcga cgtagccggc gtacaccggt tcgacgccgg
     3301 gaaacagcct ttggcggccc gttgagagca ctccatcagc gagcacgagc aggtcgtacc
     3361 ggagctgccg cccgctggcg aagtgcagat ccacgccctg cccgtcctgg ccgaacgcga
     3421 ccagcgcctc cccctgcaaa tagcgttcgc ggccgaactc gccgagaagc gcccggtaca
     3481 agctgcccca ggaaacgaaa cgatacgcga cgggttcctc ccaaagcagg gacccggctc
     3541 cgtcgaggag ccggtagaga aggaccggaa cggtcatcc
//
LOCUS       DBNGWZ010000047         8384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_633962, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000047 DBNGWZ010000000
VERSION     DBNGWZ010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8384
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_633962"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16CX_01465"
     CDS             complement(<1..311)
                     /locus_tag="AC16CX_01465"
                     /inference="COORDINATES: protein motif:HMM:NF027503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3011210.1"
                     /translation="MDQPIWQPSPARIADANLTAFVRHVRERWGVTADNNYAELHRWS
                     VSRPEQFWRSVWSYCGVLADEYDGPAVVDGDRMSGTRWFPEARLNFAENLLRRRDRST
                     A"
     gene            complement(341..1873)
                     /gene="lysS"
                     /locus_tag="AC16CX_01470"
     CDS             complement(341..1873)
                     /gene="lysS"
                     /locus_tag="AC16CX_01470"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3011211.1"
                     /translation="MSKTPREPAGGGNPDSGPDREKLVAERRAKLKALRDMGAAYPND
                     FKREHFAADLAANHGAHDNQALEAAPVKVKIAGRIMLKRVMGRASFATIRDTSGAIQI
                     FISDSDSGHEMHAAFKHYDIGDIIGAEGELFRTRTGELTVRARRLRLLAKSLRPLPEK
                     WRGLTDHELKYRQRYVDLIVNEDTRCVFEIRSRIVNAIREFLLGERYLEVETPMMQPI
                     PGGAAARPFVTHHNALDLDLYLRIAPELYLKRLVVGGYERVFEINRNFRNEGISTKHN
                     PEFTMLEFYAAYTDYEYMMAVIPRMLLHAAQTALGGPRFSYQGHEIDLSRPYERLTIT
                     RAIKKYHPEVTDSLLNDRAALIARLNQHHIAYRPADGIGGLQLTLFENTVEDKLIQPT
                     FIVDYPTEVSPLARRSDRNPDIAERFELYIAGREIANGFSELNDPDDQRSRFEQQARQ
                     KEAGDAEAMHFDADYIRALEFGMPPASGAGIGIDRLVMLLSDSPSIRDVILFPLLRPE
                     SE"
     gene            complement(1879..2983)
                     /gene="prfB"
                     /locus_tag="AC16CX_01475"
     CDS             complement(join(1879..2901,2903..2983))
                     /gene="prfB"
                     /locus_tag="AC16CX_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293703.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR3011212.1"
                     /translation="MEAEQINAIERHLKQIGSRTEDLWRYLDIEGKKERLEIVKKQFE
                     DPNIWADKKRAGELGKEKKQLESVVDTLSTTLKAVNDSVALFELARGESDDATLAAVG
                     EDAAKLEKTVAELEFKRMFSNPLDPGNCFLDIQAGSGGTEAQDWAQMLLRMYTRYCER
                     KGFRVELLEESPGEVAGIKSAALKVSGDYAYGHLRTETGVHRLVRKSPFDANNRRHTS
                     FASVFVYPEVDETIEIEINPADLRVDTFRASGAGGQHVNKTDSAIRITHLPTNIVVQC
                     QNDRSQHRNRAEAMAMLKSRLYEMELRKRQEEKQALEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTNIEIGNTQAVLDGDLDDFIEASLKRGL"
     gene            complement(3025..4839)
                     /gene="recJ"
                     /locus_tag="AC16CX_01480"
     CDS             complement(3025..4839)
                     /gene="recJ"
                     /locus_tag="AC16CX_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3011213.1"
                     /translation="MPGTTSWNGTDAAPTITTRTSRPADVERLVREGVHPLLARVCAA
                     RGVTSSRHLEAGFEHLATPDTMLNLREMAKRLADAMASRKKLLIVADYDADGATACAV
                     GVRALRAFGAAVEFLVPNRFEYGYGLSPEIVRLAHARFHPDILITVDNGIASVEGVAE
                     ANRLGMATLITDHHLPGECLPDAACIVNPNQPGCAFPSKHLAGVGVMFYLMLALRAEL
                     RERGAFSQSRKAKVERRKENDLDSDFRLPTSDLKSDEPNLADLLPLVALGTVADVVAL
                     DHNNRILVHQGLARMRADRMQAGVAALFQIAQRDPRRASARDLGFVLGPRLNAAGRLT
                     DMALGIECLVTDDTGRALAIAGELDRLNRERREIESGMQEEALAAVEARDFGDSYTLS
                     LFDPAWHPGVVGILAGRMRERFHRPVIAFAPGNPGEIRGSGRSIPSFHLRDALDRVAV
                     LNPGLILKFGGHAAAAGLTLREADFDRFRAAFEETTRSLLSPADLERVIETDGALAPE
                     ELNLDTVALLSAPVWGHGFPAPRFCDEFEVAAQRVVADKHVRLTLARGAQRLEAMRFR
                     STEAAPRRIRAVYHPTLNDYNGLQSLQLNIEHWEPAGN"
     gene            complement(4913..5875)
                     /locus_tag="AC16CX_01485"
     CDS             complement(4913..5875)
                     /locus_tag="AC16CX_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011214.1"
                     /translation="MEPYRGLDLPALETLLARGRRARTAGASLERWLAAAYRLPADLP
                     LAPYALRGESGEPGDHCWMCADPVHLKVHGDRLLLADASRFTMTPAEAQDFIDTLNEH
                     FVAEGITFIAPNPRRWYSRIAPGQNLLAAPTSEVAGRSIQEFLPAGADGARWRRILNE
                     AQMALHDHPRNQEREARGELAVNSVWLWGAGQARELPATYDVVWGDHPLAAGLAAAGK
                     AVARQLPASGAALLKEPRGKTQLAIPATLPATAYGDVAAWRGALSALERDWFAPLLAG
                     LQDSGLESIALHGLGADFCHAAEFRRHDRFRFWRRKRPLLAYAA"
     gene            6161..7402
                     /locus_tag="AC16CX_01490"
     CDS             6161..7402
                     /locus_tag="AC16CX_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR3011215.1"
                     /translation="MSFEFFIGMRYTRAKRRNHFISFISLVSVVGIALGMTVLITVLS
                     VMNGFQREIRTRILGVASHVQLSGAGGMLDDWKRIATEAARHDQVLAAAPFVSAQGLL
                     TRGSAVRGAFVRGILPEDEERVAEIGRHMRSGRLENLKPGGYGVVLGVELARALGAMT
                     GDKVVLIAPQGQVTPAGVIPRLRQFTVVGIFGVDHYEYDSGLALVHLEDAQRLFRFGD
                     AVSGVRLKLKDLFQSGRVARDLARTLGPDVYASDWTLQHANLLRAVQIEKRMMFFVVF
                     LIIAVAAFNIVSSLVMVVTDKQADIAILRTLGAAPSSITRIFIIQGTVIGIFGTLLGL
                     AGGVALAHNVETVVPIIESLFGFKFFAKDVYLISEVPSELQLPDVVLTAVFSFVISLL
                     ATVYPSYRAAQVNPAEALRYE"
     gene            7533..8234
                     /gene="lolD"
                     /locus_tag="AC16CX_01495"
     CDS             7533..8234
                     /gene="lolD"
                     /locus_tag="AC16CX_01495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HIR3011216.1"
                     /translation="MRGEEKNHGPVVACRRLRKTFHQGKVEVPVLLGVDLEVGAGERV
                     AIVGASGSGKSTLLHLLGGLDEATAGEVQVLGEDLSRQDETARGLMRNRALGFVYQFH
                     HLLPEFSALENVAMPLLIRRAGKSAAYDEAGQMLEQVGLGHRSSHRPGELSGGERQRA
                     ALARALVTRPACVLADEPTGNLDRHTAEAVFELMLELNRRSGTSLVIVTHDPDIARRA
                     DRTLRLVDGVLQPES"
BASE COUNT         1368 a         2637 c         2831 g         1548 t
ORIGIN      
        1 atggcggtgg agcggtcgcg gcgccgcagc aggttttcgg cgaagttgag ccgtgcctcg
       61 ggaaaccagc gcgtcccgga catcctgtcc ccgtccacca ccgccggccc gtcgtactcg
      121 tcggcgagca ccccgcagta cgaccacacc gatcgccaga actgctccgg ccggctgacg
      181 gaccaacgat gcagctcggc gtaattattg tccgccgtca cgccccagcg ctcgcgcacg
      241 tggcgcacga aggcggtcag gttggcgtcc gcgatgcgtg cgggggaggg ctgccagatc
      301 ggctggtcca ttgttacgag tgacgagtga cgagtcccta ttactccgat tcgggacgga
      361 ggaggggaaa caagatcacg tcgcggatcg aggggctgtc gctgagcaac atcaccagcc
      421 ggtcgatgcc gatgccggcg ccggaggcgg gcggcatgcc gaattcgagc gcccggatgt
      481 agtcggcgtc gaagtgcatg gcctcggcgt cgcccgcctc cttttgccgc gcctgctgct
      541 cgaagcggct gcgctggtcg tcgggatcgt tcagctcgga gaagccgttg gcgatctcgc
      601 gcccggcgat gtagagctcg aagcgctcgg cgatgtcggg attcctgtcg ctgcggcgcg
      661 caagcggcga cacctccgtc ggatagtcca cgatgaaggt cggctggatc agcttatcct
      721 cgacggtgtt ctcgaacagc gtcagttgta ggccgccgat gccgtccgca gggcggtaag
      781 cgatgtggtg ctggttgagc cgcgcgatca gcgcggcgcg atcgttgagc agtgagtcgg
      841 tgacttccgg atgatatttc ttgattgccc gcgtgatcgt gagacgctcg tacgggcgcg
      901 agaggtcgat ctcgtgcccc tggtagctga agcgcggccc gccgagcgcc gtctgcgcgg
      961 catgaaggag catgcgcggg atcacggcca tcatgtactc atagtccgtg taggccgcgt
     1021 agaactcgag catggtgaat tcgggattgt gcttggtcga aatgccctcg ttgcggaaat
     1081 tacggttgat ctcgaacacc cgctcgtagc cgcccaccac caggcgcttc aggtagagct
     1141 cgggcgcgat gcggaggtag aggtcgagat cgagcgcgtt gtggtgcgtc acgaatgggc
     1201 gcgcggcggc cccgccgggt atcggctgca tcatcggcgt ttcgacttcc aggtaacgct
     1261 cgccgagcag gaactcgcgg atcgcgttga cgatccgcga gcgaatctcg aatacgcacc
     1321 gcgtgtcctc gttgacgatg agatccacgt agcgctggcg gtatttcagt tcgtgatcgg
     1381 tgaggccgcg ccacttttcc ggcagggggc gcagcgattt cgcgagcagc cggagccgcc
     1441 gtgcgcggac cgtaagctcg ccagtccggg tgcggaacaa ctccccttcg gcgccgatga
     1501 tgtcaccgat gtcgtagtgc ttgaacgccg cgtgcatttc atgcccgctg tcgctgtccg
     1561 agatgaagat ctggatcgcg ccgctcgtat cccggatcgt ggcgaagctt gctcttccca
     1621 tcacgcgttt cagcatgatg cggcccgcga tcttcacctt gaccggcgct gcctccagcg
     1681 cctggttgtc atgcgcgcca tggttcgcgg cgaggtcggc ggcgaaatgc tcgcgcttga
     1741 aatcattggg gtaggcagcg cccatgtcgc gcagcgcctt cagcttggcg cgtcgctcgg
     1801 cgaccagctt ctcgcgatcc gggccgctat cggggtttcc gccgccggcg ggttctctgg
     1861 gcgttttgga catgctgtct acaaaccccg cttgagactc gcttcgatga aatcgtccag
     1921 gtccccatcc agcaccgctt gcgtgttgcc gatctcgatg ttggtgcgca ggtccttgat
     1981 gcgcgactgg tcgagcacgt aggagcggat ctggtggccc cagccgatat cggtcttggc
     2041 gtcctccagc gcctgctttt cctcctggcg cttcctgagc tccatctcgt agaggcgcga
     2101 cttgagcatc gccatcgcct ccgcccggtt gcggtgctgc gagcggtcgt tctggcattg
     2161 caccacgatg ttggtcggca ggtgcgtgat gcggatcgcc gagtcggtct tgttcacgtg
     2221 ctggccgccg gcgcccgagg cgcggaaggt atccacgcgc agatccgccg ggttgatctc
     2281 gatctcgatg gtctcgtcca cctcggggta gacgaacacg ctggcgaacg aggtatgtcg
     2341 ccggttgttg gcgtcgaagg gtgacttccg caccagccgg tgcacgccgg tctccgtgcg
     2401 caggtggccg taggcataat cgccgcttac cttgagcgcc gcgctcttga tgccggcgac
     2461 ttcgccgggc gactcctcga gcagctccac gcggaagccc ttgcgctcgc agtagcgcgt
     2521 atacatgcgg agcagcattt gtgcccagtc ctgcgcttcg gtgccgccgg aacccgcctg
     2581 gatatccagg aaacaattgc ccggatcgag cggattggaa aacatccgct tgaattcaag
     2641 ctcggcgacg gttttttcca gtttggcggc atcctctcca accgccgcca acgtggcgtc
     2701 gtcggattca ccgcgagcga gctcgaacag cgcgactgag tcgtttacgg cttttaaggt
     2761 ggttgacagc gtgtcgacga ccgattctag ctgctttttt tccttgccca gctctccggc
     2821 gcgtttcttg tccgcccaga tattgggatc ttcgaattgc ttcttgacga tttcgaggcg
     2881 ctctttcttg ccctcgatgt caaagatacc tccaaagatc ctcggtgcgg gacccgattt
     2941 gcttcaggtg cctttcaatg gcgttgatct gttcggcttc catggcggcg ttactcgaaa
     3001 aatcaaatta tacaacgaga aacatcaatt ccccgcgggt tcccagtgct cgatgttgag
     3061 ctgcagcgat tgcaggccgt tgtagtcgtt gagcgtggga tggtagacag ctcggatccg
     3121 gcgcggcgcg gcttcggtac tacggaaccg catcgcctcg agtcgttggg ctccacgggc
     3181 gagcgtgagc ctcacgtgct tgtccgccac cacgcgctgc gccgcgacct cgaactcgtc
     3241 gcagaagcgc ggcgcgggaa agccgtggcc ccataccggc gctgacagga gggcgacggt
     3301 atcgaggttg agttcctcgg gcgcaagcgc gccgtcggtc tcgatcaccc gctcgagatc
     3361 ggccggtgaa agcagtgacc gcgtcgtttc ctcgaatgcc gcgcgaaacc ggtcgaagtc
     3421 ggcctcgcgc agcgtgagac cagcggcggc ggcgtggccg ccgaacttca gtatcaggcc
     3481 cgggttcagc actgccactc ggtcgagtgc gtcgcgcagg tggaaagacg ggatcgagcg
     3541 ccccgagccg cggatctcgc ccggattgcc gggcgcgaac gcaatgacgg gacggtgaaa
     3601 gcgctccctc atgcgcccgg cgaggatgcc gaccacaccg gggtgccagg cgggatcgaa
     3661 caggctcagc gtatagctgt ctccgaaatc ccgcgcttcc acggcggcga gcgcctcctc
     3721 ctgcatgccg gactcgatct cgcgccgctc gcggttcaga cgatcgagct cgccggcgat
     3781 cgcaagtgcg cgtcccgtat cgtcggtgac caggcattcg atgccgagcg ccatgtcggt
     3841 gagccggccg gccgcgttga gccgcggccc caacacgaag cccagatcgc gcgcgctggc
     3901 gcgccgcggg tcgcgctgcg cgatctggaa cagcgctgcg acgcccgcct gcatgcggtc
     3961 cgcgcgcatg cgcgcgaggc cttggtgcac cagaatgcgg ttgttgtgat cgagcgcgac
     4021 gacgtcggcc actgtcccca gagcgacgag cgggagaagg tcagcaaggt ttggctcgtc
     4081 gctctttaag tcggaagtcg ggagtcggaa gtcggaatca agatcgtttt cctttcgcct
     4141 ttcgactttc gcctttcgac tttggctgaa cgcgccgcgc tcgcggagct cggcgcgcag
     4201 tgccagcatc aggtagaaca tcacgccgac gccggcgagg tgcttgctgg gaaaggcgca
     4261 gccgggctgg ttgggattga cgatgcacgc ggcatccggc aggcactccc ccggcaggtg
     4321 atggtcggtg atcagcgtcg ccatgcccag gcggtttgct tccgcgacgc cctccacgct
     4381 cgcgatgccg ttgtccaccg tgatcaggat atcggggtgg aatcgcgcgt gggcgagccg
     4441 cacgatttcc ggggagaggc catagccgta ctcgaagcgg ttcggcacca gaaactcgac
     4501 ggccgcgccg aaggcgcgca gcgcgcgcac gccgaccgcg cacgcggtgg cgccgtcggc
     4561 gtcgtagtct gcgacgatga gcagcttctt gcgtgatgcc atggcgtcgg cgagccgctt
     4621 tgccatctcg cgcagattga gcatggtgtc gggcgtcgcg agatgctcga agccggcctc
     4681 gaggtggcgc gaggacgtga cgccgcgcgc agcgcagacg cgcgcgagca gcgggtgcac
     4741 gccctcccgc accaggcgct cgacgtcggc gggccgggat gtgcgtgtgg tgatggtagg
     4801 cgcggcgtca gtgccgttcc aacttgtcgt tcctggcatg gagggcctgt ctacccgagg
     4861 cgatcggtgt ctgtagagca gaatggacta tcaaagaata gttggaacgg catcaggcgg
     4921 catacgcaag cagcggccgc ttccggcgcc agaagcgaaa gcggtcatgc cgccgaaact
     4981 cggcggcatg acaaaaatca gcccccagtc catgcaaggc aatcgactcc agaccggaat
     5041 cctgcaggcc cgcaagcagc ggcgcaaacc agtcccgctc cagtgcggaa agggccccgc
     5101 gccacgccgc gacgtctcca tacgccgtcg cgggcaacgt tgccggaatg gcgagttgcg
     5161 tcttgccgcg cggctccttc agcagggccg cgccggaggc gggtagttgg cgggcaacgg
     5221 ccttgccggc cgccgcaagc cccgcggcga gcgggtgatc gccccacacc acgtcgtagg
     5281 ttgcgggaag ctcgcgggcc tgtcccgctc cccacaacca gacgctgttg accgcaagct
     5341 cgccacgcgc ttcgcgctcc tggttgcggg gatggtcgtg cagcgccatt tgcgcttcat
     5401 tgagtatcct gcgccagcgc gcgccgtccg cgcccgctgg gagaaattcc tggatgcttc
     5461 tgcctgcaac ctcggacgtt ggggcggcaa gcagattttg cccaggtgcg atgcgcgagt
     5521 accagcgccg cggattcggc gcgatgaaag tgatgccctc ggcgacaaag tgctcattga
     5581 gagtatcgat aaaatcctgt gcttccgcag gcgtcatcgt aaaccgcgag gcgtccgcca
     5641 gcaggaggcg atcgccgtgc accttgagat gaacgggatc ggcgcacatc cagcagtgat
     5701 cgcccggttc gccgctctcg ccgcgcaacg cgtacggcgc aagcggcaga tcggcgggca
     5761 gacggtaggc ggcggcaagc cagcgctcga gggaggcgcc cgcagttcgg gcgcgccgcc
     5821 cgcgcgcgag tagggtttcc agcgccggca gatcgagtcc gcgatacggt tccacgcccg
     5881 cggccgcagg ccagaatagg ttcgggacga gcaagtggca attcataagg gccgctgcgc
     5941 gttgcccgca gtctagcacg gcgttcccgc tgtcctcaca ccgcgaatga gcctcgggat
     6001 cggcttgacg acagtgactg caggttgatt gttactcccg atttccagtt gcaggccttc
     6061 gtcaggaatt tgtcggactg ggtccgacga atacctcttg tgtcagaata acggcctttg
     6121 acgcgccgcg gggacacccc gtgcgggaaa ggcggcccgg ttgtcattcg agttcttcat
     6181 cggcatgcgt tacacgcgcg ccaagcgccg taaccatttc atctccttca tctcgctcgt
     6241 ctccgtcgtc ggcatcgcgc tcggcatgac ggtgctgatc acggtgctgt ccgtcatgaa
     6301 cggattccag cgcgagatcc gcacgcgcat tctgggcgtg gcctcccacg tgcaattgag
     6361 cggcgcgggc gggatgctgg acgactggaa gcgcatcgcc actgaagccg cgcgccacga
     6421 ccaggtgctc gccgcggccc ccttcgtttc cgcgcagggg ctgcttaccc gcggctcggc
     6481 ggtccggggg gcgttcgtgc gcggaatcct gccggaggac gaggagcgcg tcgctgagat
     6541 cggcaggcac atgagaagcg gccgcctcga aaacctgaaa ccgggcgggt acggcgtcgt
     6601 gctgggcgtc gagctcgcgc gggcgctggg tgcaatgacg ggtgacaagg tggtactgat
     6661 cgcgccgcag ggacaggtga cgccggccgg cgtcattccg cggctgaggc agttcaccgt
     6721 ggtgggcatc ttcggggtgg accattatga atacgattcc gggctcgcgc tggttcacct
     6781 cgaggacgcc cagcggctgt tccgtttcgg cgacgcggtg tcgggggtgc ggctcaaatt
     6841 gaaggacttg ttccagtccg gacgggtggc gcgcgatctc gcgcgtacgt tggggccgga
     6901 cgtttatgcc agcgactgga ccctgcagca cgcaaacctg ctgcgcgcgg tgcagatcga
     6961 gaagcgcatg atgttcttcg tcgtgttcct gatcatcgcg gtcgcggcgt tcaacatcgt
     7021 ctcgagcctg gtgatggtcg tcaccgacaa gcaggcggac atcgcgatat tgaggacgct
     7081 gggcgccgcg ccctctagca tcacccgaat cttcatcatc cagggcacgg tgatcggcat
     7141 cttcggcacg ctcctcggcc tcgccggcgg cgtcgcgctg gcgcacaacg tcgaaaccgt
     7201 ggtgccgatc atcgagagcc tgttcggctt caagttcttc gccaaggacg tctatctcat
     7261 ctccgaagtg ccttcggagc tgcagctgcc cgacgtcgtg ctgacggcgg tgttttcttt
     7321 cgtgatcagc ctgctcgcga ccgtttaccc gagctaccgg gcggcccagg tcaatcctgc
     7381 ggaggcgctc cgctatgagt gaggggagcg cgtcgagggg cggaagaggg gccccaatgt
     7441 ttgtaaattg gggatctgac ccccggcggc gcgcagagcg gccgacgtgt ttagatcggg
     7501 atttacgatg acatatcaga ggacgctgtc gagtgagggg tgaggagaaa aaccatgggc
     7561 cggtggttgc gtgccgcagg ctgcgtaaga ccttccacca gggaaaagtg gaggtgccgg
     7621 tgctgctggg cgtggatctc gaggtcggcg cgggcgaacg cgtggcgatc gtcggcgcat
     7681 cgggctccgg caagagcacg ctgctccatc tgctcggtgg actggacgaa gcgactgcgg
     7741 gcgaggtaca ggtgctcggc gaggacctga gccggcagga tgaaacggct cgcggcctga
     7801 tgcgcaaccg cgctctcggc ttcgtctacc agttccatca cctgctgccg gaattttcgg
     7861 cgctggaaaa cgtggcgatg ccgctgctca tccggcgcgc gggaaaaagc gcggcttacg
     7921 acgaagccgg acagatgctg gagcaagtcg gcctcgggca ccgcagctcg cacaggccgg
     7981 gggagctttc cggcggcgag cgccagcgcg ccgcgctcgc gcgcgcactg gtcacgcggc
     8041 cggcctgcgt gctcgcggac gagcccaccg gcaacctcga ccggcacacg gcggaggcgg
     8101 tgttcgagct gatgctcgag ctcaatcgcc gcagcgggac gagcctcgtg atcgtgacgc
     8161 acgatcccga catcgcacgc cgcgccgacc gcacgctccg gctggtcgac ggagtcctcc
     8221 agccggagtc gtgaggcgtg ataagtgata aggctcccgc gctcgcgcgg agctaaactc
     8281 ctatcaccta tcacctatcc cttatcaccc attacggggg tgacgcgcaa caagccgtct
     8341 ttcgtgactt cctcgtagac ggcctcatcc tgcagcacct gctg
//
LOCUS       DBNGWZ010000048         2950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2145075, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000048 DBNGWZ010000000
VERSION     DBNGWZ010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2145075"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="AC16CX_01500"
     CDS             <1..712
                     /locus_tag="AC16CX_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011217.1"
                     /translation="YGALGPMLAASLVSLVMPRGRTRAPGKPAPALLDFRPVLRNRET
                     LPYIAGYSAHCYELFGQRSWMVAFLVFTASLQPADAPMLVSAATLAATINLMGPVMSV
                     SGNELAIRFGRTRVIFTFMTASGLVACVLGYTAALPWLLVFLIMCVHYGLMLGDSAAL
                     TSGAVASAHPDERGATMAIYSFAGFSSAFLAPLVFGVVLDLAGGNRSLLAWGLAFASV
                     GIFGVLAPVARLIHTRRA"
     gene            631..930
                     /locus_tag="AC16CX_01505"
     CDS             631..930
                     /locus_tag="AC16CX_01505"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR3011218.1"
                     /translation="MGARLCERRHLRRACAGGAADPYTARLNVRLPAGCAAATGCYQP
                     PVGINVFVVKGVAEDVPMGKIFAGIMPFWAAMVACIALLIAFPQIVLVLPDSMIR"
     gene            971..2086
                     /locus_tag="AC16CX_01510"
     CDS             971..2086
                     /locus_tag="AC16CX_01510"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR3011219.1"
                     /translation="MKGKPPFRADHVGSLLRPKPLLDARERRAREEITPAELRKAEDA
                     AIRDAVRLQEEAGLQGVTDGEYRRTYFHIDFLEQLEGVAVSGGLAVKFHTAKGEVDFA
                     PPRLEVTGRLRRTRPIQLDDFNYLKSVTKRTPKVTIPSPTMLHFRGGRGGISRSAYPD
                     LELFFEDLARCYREEIASLAAAGCRYLQLDDTNLAYLCDPRLREGAKERGDDPDELPH
                     RYARLINAAIRDRPADMTACVHLCRGNFKSAWVAEGGYDPVAEALFNELEVDGYFLEY
                     DDARSGDFTPLRFIPKGRKVVVLGLVSSKLGELEAKDALKRRIDEAARFVPLEQLALS
                     PQCGFSSTVHGNDISAAQQYAKLRLVVETAREVWGTM"
     gene            2196..>2950
                     /locus_tag="AC16CX_01515"
     CDS             2196..>2950
                     /locus_tag="AC16CX_01515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011220.1"
                     /translation="MSRLDGKVAIVTGAGSGIGRALATRLARDGAAIVVADIRNHDIA
                     AAAIADATGARTLGLPVDVSSEPDAARMAEETMKTFGRIDILVNNAALFSALEPRPFE
                     RIDAAEWRKVMEVNTLGVFLCCRACVPHMRAGGGGRIINLASGAPLKGVPLLLHYVSS
                     KGAVIAFTRALAREVGRDGITVNALAPGFTLSEGVLKSAAHVKHGEATRATRAIARDE
                     RPNDLVGAVSFLASEDAAFITGQTLVVDGGSAMV"
BASE COUNT          444 a         1004 c         1017 g          485 t
ORIGIN      
        1 ttacggcgcg ctgggaccga tgctcgctgc gtcgttggtc agcctcgtga tgccccgcgg
       61 gcgcacgcgc gcgccgggca agccggcgcc ggcgttgctc gatttccggc cggtgttgcg
      121 gaaccgggaa acgctgccct acatcgccgg gtattccgcg cactgctacg aactgttcgg
      181 tcagcgttcg tggatggtcg cattcctcgt cttcaccgcg tcgctgcagc cggccgatgc
      241 gccgatgctg gtgagcgcgg cgacactcgc cgcgacgatc aatctcatgg ggccggtgat
      301 gagcgtctcg ggcaacgagc ttgcgatacg cttcggccgc acgcgcgtga tcttcacctt
      361 catgaccgcc tcgggcctcg tcgcctgcgt gctgggctac accgccgcgc tgccgtggct
      421 gctggtgttc ctcatcatgt gcgtgcacta cggcctgatg ctgggcgatt ccgccgcgct
      481 cacctccggc gcggtcgctt ccgcccatcc cgacgagcgc ggcgcgacga tggcgattta
      541 ttccttcgcg ggattcagct cggcgttcct cgccccgctc gtgttcggtg tcgtgctcga
      601 tctcgcgggc ggcaaccgca gcctgctcgc atgggggctc gcctttgcga gcgtcggcat
      661 cttcggcgtg cttgcgccgg tggcgcggct gatccataca cggcgcgcct gaacgtccgt
      721 ctcccggctg gttgcgctgc agcaacgggc tgttatcagc ccccggtcgg gatcaacgtc
      781 ttcgtggtca aaggcgtggc ggaagacgtg ccgatgggta aaatcttcgc tggcatcatg
      841 ccgttctggg cggcgatggt cgcctgcatc gcgttgctca tcgccttccc gcagatcgta
      901 ctcgttctgc ccgactccat gatccgctga acgccccgtg cccggagcac cggtcaagaa
      961 aggaggcccc atgaaaggca agccgccatt ccgcgccgac cacgtcggca gcctgctgcg
     1021 cccgaagccg ctgctcgatg cgcgcgagcg ccgcgccagg gaggagatca cgcccgccga
     1081 gctgcgcaag gccgaagacg cggcgatccg cgatgccgtg cgcctgcagg aggaggccgg
     1141 cctgcagggc gtgaccgacg gcgagtaccg ccgcacctat ttccacattg attttctgga
     1201 gcagctcgaa ggcgtcgccg tgagcggggg gctcgcagtg aagttccaca ccgcgaaggg
     1261 cgaggtcgac tttgcacccc cgcggctgga agtgaccggc aggctgcggc gcacgcggcc
     1321 gatccagctc gatgacttca actacctcaa gtcggtcacc aagcgcacgc ccaaggtgac
     1381 gattccctcg ccgaccatgc tgcatttccg cggcggccgg ggcggcatca gccggtcggc
     1441 ctacccggac ctggagctgt tcttcgagga cctggcgcgc tgctaccgcg aggagattgc
     1501 gtcgctcgcc gcggccggtt gccggtatct ccagctcgac gacaccaatc tcgcctatct
     1561 gtgcgatccc aggttgcgcg agggcgcaaa ggagcgcggc gacgatccgg acgagctgcc
     1621 gcaccgctac gcgcgcctga tcaacgccgc catccgggac cggcccgcgg acatgacggc
     1681 ctgcgtgcac ctgtgccgcg gcaacttcaa gagtgcctgg gtggccgagg gcggctacga
     1741 cccggtggcg gaggcgttgt tcaacgagct cgaggtggat ggctacttcc tcgagtacga
     1801 cgacgcgcgc tcgggcgatt tcacgccgct gcgcttcatc ccgaaaggcc gcaaggtcgt
     1861 ggtgctgggt ctcgtcagca gcaagctcgg cgagctcgaa gcgaaggacg cgctcaagcg
     1921 ccgcatcgac gaggcggcga ggttcgtccc actggaacaa ctcgcgttga gcccgcagtg
     1981 cggcttttcc agcacggtgc acggcaacga catcagcgcc gcccagcagt acgccaagct
     2041 gcgcctcgtg gtcgagaccg cgcgcgaggt ctgggggacg atgtgaaggg gaggccggcg
     2101 atgagcccgc agctcgaccg cgcgcggcgc gtgcaccgcc atcactacgc gccgatgacg
     2161 accggcatga gcctgatcac cttcgagggc gcgcagtgag ccggctcgac ggcaaggttg
     2221 cgatcgtcac cggcgccggc agcgggatcg gaagagcgct cgcgacgcgc ctcgcgcgcg
     2281 acggggcggc catcgtcgtc gcggatatcc ggaatcacga cattgcggcg gcggcaatcg
     2341 cggacgcgac gggcgcgcgc acgctgggct tgccggtgga cgtttcctcc gagccggacg
     2401 ccgcgcggat ggcggaggaa acgatgaaga catttggccg tatcgacatc ttggtcaaca
     2461 acgccgcgct gttctccgcg ctcgagccac gcccgttcga gcgcatcgac gccgccgaat
     2521 ggcgcaaggt gatggaagtg aacacgctcg gcgtgttcct gtgctgccgc gcctgcgtgc
     2581 cgcacatgcg cgccgggggc ggcgggcgca ttatcaatct tgcttccggc gcgccgctga
     2641 agggcgtgcc gctgctgctg cactacgtgt cgagcaaagg cgcggtgatc gctttcacgc
     2701 gggcgctcgc gcgcgaggtc ggaagagacg gcatcacggt gaacgcgctc gcccccggct
     2761 tcacgctttc cgagggcgtc ctgaagagcg ccgcgcacgt gaagcacgga gaagccaccc
     2821 gcgccacccg tgccattgcg cgcgacgagc ggcccaacga cctcgtcggc gcggtgagct
     2881 tcctcgcgag cgaggacgcg gccttcatca ccggccagac gctggtggtg gacggcggct
     2941 cggcgatggt
//
LOCUS       DBNGWZ010000049         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1641585, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000049 DBNGWZ010000000
VERSION     DBNGWZ010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1641585"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="AC16CX_01520"
     CDS             <1..791
                     /locus_tag="AC16CX_01520"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR3011221.1"
                     /translation="LGLPPPEPARPPYAPDLEAQFAAAEEIRPPVLTFHLGVMQHERV
                     RRFQALGVKVGGSATCVAEAERLEALGVDFIVAQGAEAGGHRGTYLRDPCDAMTGTFA
                     LVRLIVRRVRTPVVAAGGIMDGAGVAAALALGAQAAQLGTAFIPCPESGAPRVHKERI
                     LAAREDETLITEKFSGKPARGLANRFMREMHQRAAPQLLFPAQNTLTGKLRAAAAKAG
                     NPDFVAMWAGQAVALSRALPAAELVAALEAEALECLDRLAALRR"
     gene            907..>2066
                     /locus_tag="AC16CX_01525"
     CDS             907..>2066
                     /locus_tag="AC16CX_01525"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3011222.1"
                     /translation="MKDDPIVIVGSARTPMGGFLGDLKSLSAPELGAAAIRAAVERAG
                     VEPGDIQEVIMGNVLPAGQGQAPARQAALGAGLPPAAGCVTINKVCGSGMKAAMLAHD
                     IIKVGAQSVMVAGGMESMSNAPYLLPKARAGLRLGHGDVKDHMFLDGLEDAYDRGRLM
                     GTFAEDCADKYRFSREAQDRYAITSLTRAQEAIRNGSFAREIVPVAIAGGKTAAKVER
                     DEQPPKANLDKIPQLKPAFRKDGTVTPANSSSISDGAASMVLMRLSEAERRGIAPLAR
                     VAGYATHSQEPQWFTTAPVGAIRKLFEKTGWDAKTTELFEINEAFAVVAMAAMHDLDL
                     PHDKVNVHGGACALGHPLGASGARIMVTLLAALEKYGLKRGIAALCIGGGEAT"
BASE COUNT          347 a          704 c          716 g          299 t
ORIGIN      
        1 agctgggtct gcccccgccc gagccggcgc gcccgcccta tgcgccggac ctcgaggcgc
       61 agtttgccgc ggcggaggaa atacgcccgc cggtgctcac cttccacctc ggcgtcatgc
      121 agcacgaacg ggttcgccgc ttccaggcgc tcggtgtgaa ggtcggcggc agcgcgacct
      181 gcgtcgccga agccgaacgg ctcgaagcgc tcggcgtgga cttcatagtc gctcaaggag
      241 cggaggcggg cggccatcgc ggcacctatt tgcgcgatcc ttgcgacgcg atgaccggca
      301 cctttgccct ggtgcggctg atcgtgcgca gggtgaggac gccggtcgtc gccgcgggcg
      361 gcatcatgga cggcgcaggc gttgccgccg cgctcgcgct cggcgcccag gcagcgcagc
      421 tcggcaccgc cttcatcccc tgtcccgaaa gcggcgcgcc gcgcgtgcac aaggagcgca
      481 ttctcgccgc gcgggaggac gaaaccctga tcacggaaaa gttctcgggc aagcccgcgc
      541 gcggcctcgc caaccgcttc atgcgcgaga tgcaccaacg cgcggccccg cagcttctct
      601 ttccggcgca aaatacgctc accggcaagc tgcgtgcggc ggcggcgaag gccggcaacc
      661 ccgatttcgt cgcgatgtgg gccggtcagg cggtcgcgct ctcccgcgcg ctgcccgcgg
      721 cggagctcgt ggcggcgctc gaggccgagg cgctcgaatg cctggaccgg ctcgccgcgc
      781 tgcgccgcta agaacacaag aatcttgaac cacaagggac acaaaaaata acgaaaacaa
      841 gagagtcatc ttgaataaaa ccttcgtgtc ctttgtggtt aagcttttgg tgttaaggag
      901 tcggacatga aagacgaccc catagtgatt gtcggttccg cccgcacgcc gatgggcgga
      961 tttttgggag atctcaaatc gctctccgcc cccgagctcg gagcggccgc catccgcgcc
     1021 gccgtcgagc gggcgggggt cgagcccggt gacatccagg aagtgatcat gggcaacgtg
     1081 ctgcccgcgg gccaggggca ggcgccggcg cgccaggccg cgctcggtgc ggggttgccg
     1141 cccgccgccg gctgcgtgac catcaacaag gtgtgcggct cgggcatgaa ggcggcgatg
     1201 ctggcgcacg acattatcaa ggtgggcgcc caatccgtga tggtcgcggg cggcatggag
     1261 agcatgtcga acgcccccta cctcctcccc aaggcgcgcg ccggcttgcg cctggggcac
     1321 ggtgacgtga aggaccacat gttcctcgac gggctggagg acgcctatga ccgcgggcgc
     1381 ctgatgggca cgttcgccga ggactgcgcc gacaagtatc gcttctcccg ggaagcgcag
     1441 gaccgctacg ccatcacttc cctcacccgc gcgcaggagg cgatcaggaa cggcagcttt
     1501 gccagggaga tcgtgccggt cgcgatcgcc ggcggcaaga ccgcggcgaa ggtcgagcgg
     1561 gacgagcagc cgcccaaggc caacctcgac aagatccccc agctcaagcc cgcgttcagg
     1621 aaggacggga ccgtcacgcc cgccaattcg agctccattt ccgacggcgc ggcgtcgatg
     1681 gtgctgatgc ggctctcgga ggcggagcgg cgcggcatcg cgccgctcgc ccgggtggcc
     1741 ggctacgcca cgcattcgca ggagccgcaa tggttcacca cggcgccggt gggcgcgatc
     1801 cgcaagctct tcgagaagac cggctgggac gcgaaaacga ccgagctttt cgagatcaac
     1861 gaagccttcg ccgtggtggc gatggcggcg atgcacgacc tcgacctgcc gcacgataag
     1921 gtcaacgtcc acggcggcgc atgcgcgctc ggacatccgc tcggcgcgtc cggcgcccgc
     1981 atcatggtga cgctgctcgc cgcgctcgag aagtacgggc tgaagcgcgg catcgcggcg
     2041 ctgtgcatcg gcggcggtga ggccac
//
LOCUS       DBNGWZ010000050         3489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3278665, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000050 DBNGWZ010000000
VERSION     DBNGWZ010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3278665"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /gene="gabD"
                     /locus_tag="AC16CX_01530"
     CDS             complement(<1..1466)
                     /gene="gabD"
                     /locus_tag="AC16CX_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - GABA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3011223.1"
                     /translation="MLQKVKPAVQLKDMSLFRQQCYINGGWVDADNKSTLAVYNPADS
                     QQIGTVPNMGAAETRRAIETAHAAWPAWRAKTAKERAAILRKWYELMMANQEDLAVLM
                     TVEQGKPLTESRGEIAYGASFIEWFAEEAKRVYGDTIPAQANDRRIVVLKQPIGVCAA
                     VTPWNFPNAMITRKAGPALAAGCTMVIKPASMTPYSALALCELAERAGVPRGVLSVVT
                     GASGPIGKELTTHPLVRKFTFTGSTEVGKKLMEQCAGTVKKVSLELGGNAPFIVFDDA
                     DIDAAIEGALASKFRNTGQTCVCANRILVQDGVYDRFAKKLADKVSQMKVGNGLESGV
                     VQGPLIEMKAVEKVEEHVADAVAKGAKVITGGKRHEKGGQFFQPTVLANVTTAMKVTR
                     EETFGPVAPLYRFKTEDELLKLANDTEYGLAAYFYSRDIGRVWRVAEGLESGIVGINI
                     GIISTEVAPFGGVKESGIGREGSKYGIEEFLEVKYLCMG"
     gene            1661..2587
                     /locus_tag="AC16CX_01535"
     CDS             1661..2587
                     /locus_tag="AC16CX_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR3011224.1"
                     /translation="MRALVACGTVSLLLLGGCASTGNPHDPLEPINRGIYKFNDGVDH
                     LIIRPAAEIYRGVLPDFARKGISNFFSNINDVIVALNNLLQGKFTNAISDVGRIVVNT
                     TVGLLGLIDVASELGLEKHDEDFGQTLGYWGIGTGPYLVLPILGPSSMRDTVGLVGDW
                     KTNPLTYVDPNRDRNALYGLNIISRRSELLEASDILATAALDPYEFVRDGYLQRRRNL
                     VYDGNPPDENGNEIKEKPKPGASGSSSFPAPADAGYGSLLVSGEALPTPAELEARERA
                     MHAAAPAPPPPTTYEAPKQTRVVRVWLPSGRN"
     gene            complement(2704..>3489)
                     /locus_tag="AC16CX_01540"
     CDS             complement(2704..>3489)
                     /locus_tag="AC16CX_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024762.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR3011225.1"
                     /translation="IDLAEAALVIAAHEYPGLDIAAYLARIDEMAATLKRRLRRDIGP
                     TETLIALNRYFFDELGFSGNAADYYNPRNSFLNDVLDRRLGIPITLSLLYTEIGRRVG
                     LALHGVSFPGHFLVKCTVRDGAVVLDPYARGASLSLDDLQQRLRALRGGALPAPEMVR
                     HLLAAAGKKGILARMLRNLKGIYLERRDFVRALAAADRIIGLEPGAAEEYRDRAGIYL
                     DLECFRAALSDFRNYLMLKPGAEDAAVVQRRVVELQQLAARLN"
BASE COUNT          586 a         1174 c         1093 g          636 t
ORIGIN      
        1 cccatgcaga ggtacttcac ctcgaggaac tcctcgatgc cgtacttcga gccctcgcgg
       61 ccgatgccgg actccttcac cccgccgaat ggcgccacct cggtcgagat gatgccaatg
      121 ttgatgccga cgatgccgga ttccagcccc tccgccacgc gccagacacg gccgatgtcg
      181 cggctgtaga agtaggcggc aagcccgtac tcggtgtcgt tggcgagctt cagcagctca
      241 tcctcggtct tgaaacggta gagcggcgcg accggaccga aggtctcctc gcgcgtgacc
      301 ttcatcgcgg tcgtgacgtt ggcgagcacg gtcggctgga agaactggcc gcccttctca
      361 tgacgcttgc cgccggtgat aaccttcgcg cctttcgcga ccgcgtccgc aacgtgctcc
      421 tcgaccttct ccaccgcctt catctcgatg agcggcccct gcacgacgcc ggactcgagg
      481 ccgttgccca ctttcatctg ggacaccttg tcggcgagtt tcttcgcgaa cctgtcgtag
      541 acgccgtcct gcaccaggat gcggttcgcg cacacgcagg tttgccccgt gttgcgaaac
      601 ttggaggcca gcgcgccttc gatcgcggcg tcgatgtcgg cgtcgtcgaa cacgatgaag
      661 ggcgcgttgc cgccgagctc gagcgacacc tttttcaccg tgccggcgca ttgctccatc
      721 agcttcttgc cgacctcggt cgagccggtg aaggtgaatt tccgcaccag cgggtgggtg
      781 gtgagttcct tgccgatggg gccggaggcg ccggtcacga ccgacagcac gcccctgggc
      841 acgccggcgc gctccgccag ctcgcacagc gcgagcgccg agtacggcgt catgctcgcg
      901 ggcttgatca ccatggtgca gccggccgcc agcgccgggc ccgccttgcg cgtgatcatc
      961 gcattgggga aattccatgg cgtcaccgcc gcgcacactc cgatcggctg cttcagcacc
     1021 acgatgcgcc ggtcgttcgc ctgcgccggt atggtgtcgc cgtacacgcg cttggcttcc
     1081 tcggcgaacc actcgatgaa ggaggcgccg taggcgatct cgccgcggga ttccgtgagc
     1141 ggcttgccct gctccaccgt catcagcacc gcgaggtcct cctggttcgc catcatgagc
     1201 tcgtaccatt tgcgcaggat cgcggcgcgc tccttcgcgg tcttcgcgcg ccacgccggc
     1261 cacgccgcgt gcgccgtttc gatcgcgcgg cgggtctccg ccgcgcccat gttgggcacg
     1321 gtgccgatct gctggctgtc ggccgggttg tagacggcaa gggtggattt gttgtcggcg
     1381 tccacccacc cgccgttgat atagcattgc tggcggaaca ggctcatgtc cttcaactgc
     1441 accgcgggtt tgactttctg cagcatggcg gaagtcctcg tgtcgaatcc tgggaaggtc
     1501 gtgacaatgc aggcggcaag ccggcccgct tcagagttgt tagtataaca gagcgtgcgc
     1561 gggcaggcgt cttacggcag cacaaaaaag cttgatataa taaatcgtta aacaacaaag
     1621 cccccgctca atgctcgctc tcggtggccc cttatcgcgc ttgcgcgcac tggtcgcgtg
     1681 cgggaccgtt tcccttcttc ttctcggcgg ctgcgcgagc accggcaatc cgcatgatcc
     1741 gctcgagccg atcaaccgcg gcatctacaa attcaacgac ggcgtcgatc acttgatcat
     1801 caggccggcc gccgaaatct accgcggcgt gctgccggat ttcgcgcgca aggggatcag
     1861 caatttcttc tcgaatatca acgacgtgat cgtcgcgctg aacaacctgc tgcagggcaa
     1921 gttcacgaat gcgatctccg acgtgggccg catcgtggtc aataccaccg tcggtctgct
     1981 ggggttgatc gacgttgcga gcgagctggg gctggaaaag cacgacgagg acttcggcca
     2041 gacgctgggc tattggggca tcggcaccgg cccttacctg gtcctcccga tcctcggccc
     2101 gagctcgatg cgcgacaccg tgggcctggt cggggactgg aagacaaatc cgctcactta
     2161 cgtggatccc aaccgcgacc gcaacgcgct ctacggattg aatataatca gccgccgctc
     2221 cgagctgctc gaagcgagcg acatactcgc aacggccgcg ctcgatcctt acgaattcgt
     2281 gcgcgatggc tacctgcagc gccgccgcaa cctggtctac gacggcaacc cacccgacga
     2341 gaatggcaac gagatcaaag agaagcccaa accgggtgcc tcgggttcgt catccttccc
     2401 ggctccggcg gacgccggtt acggctcgct actggtgagc ggcgaggcgt tgcccacgcc
     2461 ggccgaactg gaggcgcgcg agcgcgcaat gcacgcagcg gcccccgccc cgccgccacc
     2521 caccacctac gaagcaccga agcagacgcg tgtcgtgcgc gtctggctgc cctccggccg
     2581 caactaggcg gcgcgcggcg ccgctcgtta ctcgttactc gttgctcgtt gttggtcgat
     2641 gatcgactgg ctagtgcgcg gtattttctc gagtaacgaa taacgagcaa cgagtcacga
     2701 tttctagttg agccgggcgg cgagctgctg cagctccacc accctgcgct gcaccaccgc
     2761 ggcgtcttcc gctcccggct tcagcatgag gtagttgcgg aaatccgaca gcgctgcgcg
     2821 aaagcattcc aggtcgaggt atatgccggc gcgatcgcgg tactcctccg ccgctccggg
     2881 ctcgagcccg atgatgcggt cggccgccgc aagggcgcgg acgaaatccc gccgctccag
     2941 gtagatgccc ttcagattgc gcagcatgcg cgccaggatg cctttcttgc cggcggcggc
     3001 cagcagatgc cggaccatct ccggggcggg cagcgcgccg ccgcgcaatg ccctcaatcg
     3061 ttgctgcagg tcatccaggc tcagcgaggc gccgcgcgca tacgggtcca gcacgaccgc
     3121 cccgtcgcgc acggtgcatt tgaccaggaa gtgccccggg aacgaaacgc cgtgcaacgc
     3181 gagcccgacg cgccgcccga tctccgtgta gaggagggaa agcgtgatcg ggatgccaag
     3241 ccggcggtcg agcacgtcgt tgagaaagct gttgcgcgga ttgtaataat ccgcggcgtt
     3301 gccgctgaag ccgagctcgt cgaaaaaata gcggttgagc gcgatgagcg tctcggtggg
     3361 gccgatgtcc cggcgcagcc tgcgcttcag cgtcgccgcc atctcatcga tgcgcgccag
     3421 gtacgccgcg atatccagtc cggggtattc gtgcgcggcg atgaccagcg ccgcctcggc
     3481 gaggtcgat
//
LOCUS       DBNGWZ010000051         3404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2901056, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000051 DBNGWZ010000000
VERSION     DBNGWZ010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2901056"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /gene="hflC"
                     /locus_tag="AC16CX_01545"
     CDS             <1..527
                     /gene="hflC"
                     /locus_tag="AC16CX_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR3011226.1"
                     /translation="TINDGMRAEFGNRTVHEVVSGERDKIMDLMREKANEDAKTIGVE
                     VLDVRLKRVDLPQEVSVDVYRRMEAERKRVANELRSTGAAESEKIRADADRQREVIIA
                     QAYREAQRIKGEGDGKATATYARAYEQNPEFYAFYRSLEAYKASFRGRNDVLVLEPNS
                     DFFKYLRSSGRPAK"
     gene            599..787
                     /locus_tag="AC16CX_01550"
     CDS             599..787
                     /locus_tag="AC16CX_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR3011227.1"
                     /translation="MGNILLTAFALMLIIEGVLPFLLPGLWRDTFRRLTEMSDGQIRF
                     IGLTSMLAGLLLLYFVRQ"
     gene            891..2054
                     /locus_tag="AC16CX_01555"
     CDS             891..2054
                     /locus_tag="AC16CX_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR3011228.1"
                     /translation="MRTWVLPEYIEDILPLEAARIERLRRDILDLFRVHGYQLVMPPL
                     LEHLESLLTGTGHDLDLQTFKLVDRLSGRMLGVRADITPQVARIDAHLLNRKGVTRLC
                     YAGSVLHTLPSGMNRTREPLQIGAELYGHAGIESDVEIQRLMMKTLAVARVTDTYLDI
                     GHVGIFRSLVARARASGALESDLFHAMQAKDLPALRGLTRRLDRASREALIALPELYG
                     DPGALERARKILPDYPEIRAALAGLRALSEELRDVAHIRCFDLAELRGYRYHSGVVFA
                     AYAKGASNALALGGRYDEVGKAFGRARPATGFSMDLRELAAAGPNDAARSAILAPHAP
                     GDTALQRGINSLRARGEIVIVDLPGHAGSRRELGCDRKLVRRDGRWLVVKLKS"
     gene            2161..>3404
                     /locus_tag="AC16CX_01560"
     CDS             2161..>3404
                     /locus_tag="AC16CX_01560"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR3011229.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLTDHAQGVVRFQGGHNAGHTL
                     VIDGRKTVLHLIPSGILREKVACYIGNGVVLSPQALIEEIGTLEAAGVDVRPRLKISE
                     ACPLILPYHAALDQARETAKGAGKIGTTGRGIGPAYEDKVARRAIRLQDLFHRERFSK
                     KLGEVLDFHNFVLKNYFHAETVDFQKTFDETMRLSERIKPLVADVPRLLYEAQRAGHN
                     LLFEGAQGTLLDIDHGTYPFVTSSNCVAGAAAAGAGIGPQHLHYILGVTKAYTTRVGS
                     GPFPTELDDSVGRQLASRGNEFGATTGRPRRCGWFDAAALKRSIQINGVSGLGVTKLD
                     VLDGMETVQLATGYRLNGERSDILPVGAEELEGCEPLYEEMPGWKESTVGLTRMAQLP
                     KAARNYLERIEQVCGVPIDLIST"
BASE COUNT          615 a         1106 c         1147 g          536 t
ORIGIN      
        1 aaacgatcaa cgatggcatg cgcgcggagt tcggcaaccg caccgtgcac gaggtggtgt
       61 ctggcgagcg cgacaagatc atggacctga tgcgcgagaa ggcgaacgaa gacgcgaaga
      121 cgatcggcgt cgaggtgctc gacgtgcggc tgaagcgtgt cgacctgccg caggaagtga
      181 gcgtcgacgt ctatcgccgc atggaagccg agcgcaagcg ggtggctaac gagctgcggt
      241 caaccggcgc cgcggagtcc gagaaaatcc gcgccgacgc cgaccgccag cgcgaggtga
      301 tcatcgccca ggcctaccgc gaagcgcagc gcatcaaggg cgaaggcgac ggaaaggcga
      361 cggcgaccta cgcgcgcgcc tatgagcaga accccgagtt ctatgcgttc taccgcagcc
      421 tcgaggccta caaggcgagc ttccggggtc gcaacgacgt gctggtgctc gagcccaact
      481 cggacttctt caagtacctc aggtcgagcg gcaggccagc gaagtagggc ttggggcagc
      541 gtgaggcgcg aagccacctc ccgcgtgaca cgatgccggc tgccaacgtg ggacgatgat
      601 gggaaacatc ctgctcaccg cattcgcgct gatgctcatc atcgagggcg tgctgccatt
      661 cctgctcccg ggcctgtggc gcgacacctt ccggcgcctg accgagatga gcgacgggca
      721 gatccgcttc atcgggttga cctcgatgct ggcgggactg ctcctgctct acttcgtccg
      781 ccagtgagtt tcagtgagaa gtgaagagtg aggggtgagg agcaatccag gttcctcggc
      841 aatctcctct ataattcacc tcactcctca tcccttactc ctcaagcgtc atgagaacct
      901 gggtgctgcc cgaatatatc gaggacatcc tcccgctgga agccgcgcgc atcgagcggc
      961 tgcggcgcga catcctcgat ctcttccgcg tgcacggcta ccagctggtg atgccgccgc
     1021 tgctggagca cctggagtcg ctgctcacgg gcaccggcca cgatctcgac ctgcagacct
     1081 tcaagctggt ggaccggctc tcggggcgca tgctcggcgt gcgcgccgac atcacgcccc
     1141 aggtcgctcg catcgacgcg cacctcctga accgcaaggg cgtcacgcgg ttgtgctatg
     1201 caggaagcgt gttgcacacg ctgccctccg gcatgaaccg cacgcgcgag ccgttgcaga
     1261 tcggcgccga gctctacggc catgccggca tcgaaagcga cgttgaaatc cagcgcctca
     1321 tgatgaagac gctggcggtg gcgcgcgtga ccgatacgta tctcgatatc ggccacgtcg
     1381 gcatcttccg cagcctggtc gcgcgcgccc gcgcgtcggg cgcgctggaa tcggacctgt
     1441 ttcacgccat gcaggcgaaa gacctgccgg cactgcgcgg cctcacgcgc cggctcgacc
     1501 gcgcctcgcg cgaagcgttg atcgcccttc cggaactgta cggcgacccc ggagcgctcg
     1561 agcgcgcgcg caagatcctg ccggactacc ccgagatccg ggctgctctt gcgggtttac
     1621 gggcgttgtc ggaggaattg cgggacgttg cgcacatacg ctgcttcgat ctcgcggagc
     1681 tccgcggata ccgctaccac agtggcgtgg tgttcgccgc ttatgccaaa ggcgcgtcca
     1741 acgcgctggc attgggcgga cgctacgacg aggtgggcaa agcgttcgga cgcgcccgcc
     1801 cggcgaccgg cttcagcatg gacctgcgcg agctcgctgc ggcgggtccg aacgatgcgg
     1861 caaggtccgc catcctcgcc ccgcacgcgc cgggggacac cgcactgcaa cggggcatca
     1921 attctctccg cgcgcgtgga gagatcgtga tcgtggacct gccgggacac gccgggtcgc
     1981 gccgcgaact ggggtgcgac cgcaaactcg tgcgtcgcga cggcaggtgg ctcgtcgtaa
     2041 agctcaaatc ctgacacgaa gccgcgaagc cgcaaaggtg atacaaggtg atgattttcg
     2101 ttcgggtact ctttgcgcct tcgcgccttt gcgtcgaggg ttccgcgagt tgagcaagcc
     2161 atggcgaaga acgtagtcgt gatcggcgcc cagtggggcg acgagggcaa gggcaagatc
     2221 gtggactggt tgaccgatca cgcccagggc gtggtgcgct tccagggtgg acacaacgcc
     2281 ggccatacgc tggtgatcga tggccgcaag acggtgctcc atctgatccc gtccggcatc
     2341 ctgcgcgaga aggtggcttg ctacatcggc aacggggtcg tgctctcgcc gcaggcgctc
     2401 atcgaggaga tcgggacgct cgaagccgcc ggcgtcgacg tgcggccgcg gctcaagatc
     2461 agcgaagcgt gcccgctcat cctgccgtac cacgcggcgc tcgaccaggc gcgcgagacg
     2521 gcgaagggag caggcaagat cgggacgacc gggcgcggca tcggaccggc gtacgaggac
     2581 aaggtggcgc ggcgcgcgat ccgcctgcag gacctgttcc accgcgagcg tttctcgaag
     2641 aagctcggcg aggtgctcga tttccataat ttcgtgctga agaactattt tcacgcggag
     2701 accgtggact tccagaagac cttcgatgag acgatgcgac tctccgagcg catcaagccg
     2761 ctggttgcgg acgtgccgcg actgctctac gaagcgcaga gggcgggcca caacctgctg
     2821 ttcgaaggcg cacagggcac gctgctcgac atcgatcatg gcacctatcc gttcgtgacg
     2881 tcgagcaact gcgtggcggg cgcggcggct gcgggcgccg ggatcggccc gcagcacctg
     2941 cactacatac tgggcgtcac caaggcctac accacgcgcg tcggttcggg gccgttcccc
     3001 accgagctcg acgacagcgt ggggcgacag ctcgcgagcc gcggcaacga gttcggcgcc
     3061 accaccggcc ggccgcgccg ctgcggctgg ttcgacgcgg cggcgctcaa gcgctcgatc
     3121 cagatcaacg gcgtctccgg gctcggcgtc accaagctcg acgtgctcga cggcatggaa
     3181 acggtgcagc tcgccaccgg ctaccggctc aacggagagc gcagcgacat cctgccggtg
     3241 ggagcggagg agctcgaggg atgcgagccg ctctacgagg aaatgccggg ctggaaggag
     3301 agcaccgtgg ggctcacccg catggcgcag ctgcccaagg cggcgcgaaa ctatctcgag
     3361 cgcatcgagc aggtctgcgg cgtgccgatc gacctcatct ccac
//
LOCUS       DBNGWZ010000052         2657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1515889, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000052 DBNGWZ010000000
VERSION     DBNGWZ010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1515889"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC16CX_01565"
     CDS             complement(<1..682)
                     /locus_tag="AC16CX_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR3011230.1"
                     /translation="MSTLRVSVWRGTESGHFQEFEVPRHESQTVLDVVTYIQRALDPT
                     LAYRFACRVGMCGSCAMRVNGQARWTCRTHVDKVAAQSRLEVAPLANLPVVKDLVTDM
                     TQFFDKWARARGSFRGGKTRRDDFERVAPDSPARRLADAAIECIGCGVCYSACDVVAW
                     NPDYLGPAALNRAWTLLNDARDTDTRARLEAVAGDAGCHACHTHMSCTERCPKQLAPT
                     ASIAGLKRA"
     gene            complement(806..1975)
                     /locus_tag="AC16CX_01570"
     CDS             complement(806..1975)
                     /locus_tag="AC16CX_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3011231.1"
                     /translation="MISSKTTFQGYRRENGRVGIRNHVIILPVDDLSNAAAQAVENNI
                     KGTMALPHPYGRLQFGADLELHFRTLIGTGSNPNVAAVVVIGIEEQWTKRVVDGIART
                     GKPVTGFGIELHGDHDTIMRASRVAKEYLQWATELKKTECPAGDLWVSCKCGESDTTS
                     GCGSNPTVGNAFDKLEPLGVTLCFGETTEITGGEQIVADRCATPQVREKWMQVFNRYQ
                     EVIDRHKTSDLMDSQPTKGNIAGGLTTIEEKALGNIQKIGRKCKVIGVLDKAETPTGP
                     GLWFMDSSSAAAEMVTLVAAAGYVVHFFPTGQGNVIGNPILPVIKLTANPRTARTMSE
                     HIDLDVSGLLQRQKNMDQCGDELLEAMLRTCNGRLTCAEALGHREFVLTRLYESA"
     gene            complement(2031..2312)
                     /locus_tag="AC16CX_01575"
     CDS             complement(2031..2312)
                     /locus_tag="AC16CX_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR3011232.1"
                     /translation="MIQVLVHDKKDTVGVIVVEGVKAGQELTGWVMEDDSTIKINALN
                     DIPIGHKLALKDIGNGETVIKYGFDIGKAVAPIKKGEHAHVHNMKTKRW"
     gene            complement(2431..>2657)
                     /locus_tag="AC16CX_01580"
     CDS             complement(2431..>2657)
                     /locus_tag="AC16CX_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011233.1"
                     /translation="AAQFGRAADYFLRAALAGESQTADALALRARLAAGMNLARAGYR
                     EDARAQFQWLLKHSKDPSQLEAARRELSKL"
BASE COUNT          414 a          875 c          858 g          510 t
ORIGIN      
        1 cggcgcgttt gagccctgcg atggatgcgg tcggggcgag ctgcttggga cagcgctcgg
       61 tgcagctcat gtgggtatgg caggcgtgac agccggcatc gcccgccacc gcctcgagcc
      121 gcgcccgtgt gtcggtgtcg cgcgcgtcgt tcagcagcgt ccaggcgcgg ttgagggccg
      181 ccggcccgag ataatccggg ttccacgcca ccacgtcgca cgcgctatag cacacgccgc
      241 agccgatgca ctcgatcgcg gcatcggcca ggcgccgtgc gggagaatcc ggcgcgacgc
      301 gctcgaaatc gtcgcggcgc gtcttcccgc cccggaagct tccgcgcgcc cgtgcccact
      361 tgtcgaagaa ctgcgtcatg tcggtgacga ggtccttcac caccggcagg tttgcaagag
      421 gggcgacctc gaggcggctt tgcgccgcga ccttgtccac gtgggtgcgg caggtccagc
      481 gcgcctgccc gttcaccctc atcgcgcacg agccgcacat gccgacgcgg catgcgaagc
      541 ggtaggcgag cgtcggatcg agcgcgcgct ggatgtaggt gacgacatcg agcacggtct
      601 ggctttcgtg gcgcggcact tcaaactcct ggaagtgccc cgactctgtg ccgcgccaga
      661 cggatacacg cagtgtgctc atcgcgagca ccctgaaggc gaaagtcgaa aggcgaaagt
      721 cgaaaggagg cacggcaaga cgccttgcgc gctacgcgcg cccgcagcct tccgcctttc
      781 gccttccgcc ttcatccttc cgttcttacg cgctttcgta caagcgcgta agaacgaatt
      841 cgcggtggcc gagcgcttcc gcgcaggtga gccgcccgtt gcaggtgcgc agcatcgcct
      901 ccagcagctc gtcgccgcac tggtccatgt tcttctgccg ctgcagcagc cccgagacgt
      961 cgaggtcgat gtgctcgctc atggtgcgcg cggtgcgcgg gttggcggtc agcttgatca
     1021 ccggcaggat cggattgccg atcacgttgc cctggccggt ggggaagaaa tgcacgacat
     1081 atcccgccgc ggcaaccagc gtcaccattt ccgccgcggc cgaagacgag tccatgaacc
     1141 acaggcccgg gccggtcggg gtctcggcct tgtcgagcac gccgatcacc ttgcacttcc
     1201 tgccgatctt ctggatgttg ccgagcgcct tttcctcgat ggtggtgagg ccaccggcga
     1261 tgttgccctt ggtcggctgc gagtccatca ggtcgctggt cttgtgccgg tcgatcactt
     1321 cctggtagcg gttgaacacc tgcatccact tttcgcgcac ctgcggcgtg gcgcaacggt
     1381 cggcgacgat ctgctcgccg ccggtgatct cggtggtctc gccgaagcac agcgtcaccc
     1441 cgagcggctc gagcttgtcg aaggcgttgc cgacggtcgg gttggagccg cagccggagg
     1501 tcgtgtccga ctcgccgcac ttgcacgata cccagaggtc gcccgccggg cactccgtct
     1561 tcttgagctc ggtagcccac tgcaggtatt ccttggccac gcgcgaagcc cgcatgatcg
     1621 tgtcatggtc tccgtgcagc tcgataccga aaccggtcac cggtttcccg gtcctggcga
     1681 tgccgtccac gacgcgtttg gtccattgct cctcgatgcc gatcaccacc acggcggcga
     1741 cattggggtt cgagccggtg ccgatgagcg tgcggaaatg cagctccaag tcggcgccga
     1801 attgcagccg cccgtacggg tgcggcagcg ccatcgtgcc cttgatgttg ttttccaccg
     1861 cctgcgcggc ggcattcgac agatcatcca ccggcaggat gatgacgtgg ttgcggatgc
     1921 cgacgcggcc gttctcgcgg cggtagccct ggaaagtagt cttgctgctg atcatcgcga
     1981 agcccttctg aaaggtgaat catcaatggt aaaacgcgcg cgagcgcgtt ttaccagcgc
     2041 ttggtcttca tgttgtgcac gtgggcgtgc tcgcccttct tgatcggtgc caccgctttg
     2101 ccgatgtcga aaccgtactt gatcacggtc tcgccgttgc cgatgtcctt caacgcgagc
     2161 ttgtggccga tcgggatgtc gttcaatgca ttgatcttga tggtcgagtc gtcttccatc
     2221 acccagccgg tcagctcctg cccggccttg acgccctcga ccacgatgac gccgacggtg
     2281 tccttcttgt cgtgaaccag aacctgaatc atttcgactc ctgcgaacag ccggcacgag
     2341 gtggtgccgg caatgataag ggaaacagcg aatggctatc gttctagggg caatcgtagc
     2401 gcgccggctg acatcatttc aagatacttc tcacagtttg gacagctcgc ggcgcgcggc
     2461 ttcgagctgc gacgggtcct tcgagtgctt gagcagccac tgaaactgcg cacgcgcgtc
     2521 ttcccggtag ccggcgcggg cgaggttcat ccccgcggca agccgggccc gcagcgccag
     2581 cgcgtcggcc gtctgcgatt cgccggcgag cgcggcgcgc aggaagtagt ccgcggcgcg
     2641 cccgaactgg gccgcgg
//
LOCUS       DBNGWZ010000053         5802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1137981, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000053 DBNGWZ010000000
VERSION     DBNGWZ010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1137981"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="AC16CX_01585"
     CDS             complement(<1..208)
                     /locus_tag="AC16CX_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3011234.1"
                     /translation="MLKIWGRANSINVQKVLWCCAELKLEYERIDAGGKFGVTNTPEY
                     LAMNPNGLVPTIRDDGLTLWESHAI"
     gene            complement(391..1356)
                     /locus_tag="AC16CX_01590"
     CDS             complement(391..1356)
                     /locus_tag="AC16CX_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011235.1"
                     /translation="MQTRTIWYWAIAPALLLGAAAVAQNYPDRPIRTVVPASPGGVTD
                     IVARAVTPQLSEALGQSIVVDNRTGAGGIIGTDTVAKAAPDGHTLLAVFDSFISNPYV
                     FKNAPYDTIRDFAPISLLIRGPQLLVVHPALGARTFNEFLALAKSRRVPVNFATAGAA
                     TSSRLSVELFKVASGLEANLVHYRGGGPALNDLLGGHVEAMIASAGLVLPHVKAGRLN
                     VLAVSSKGRSALVPGAPSIGEFYPQFEAQSWVGLLAPAKTPRAIIRRLNVEVIKVLAA
                     PEQKERFAGMGYETVGSTPEQFGAWIRSETAKWSRIIRERNITAE"
     gene            1492..2466
                     /locus_tag="AC16CX_01595"
     CDS             1492..2466
                     /locus_tag="AC16CX_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3011236.1"
                     /translation="MAFTGIESVVFGAPDLKLARKLFSDWGLEKLSAGESGAVFETGI
                     GGRVVVRPENSRRLPPKLAGGSNFRELVWGVSSRRHFDAIAQELARDRKVSEEKDGSI
                     HATDDCGIHIGFRLGLRGKGSPRRATPFNSPAHRARVDRVAAVYERARPYRMGHIAFF
                     VPDVKTGEKFYMQRLGFWLSDRYAGGAAAFLRNAAASDHHNLFLLKSRTGATDIHHIA
                     FEVSDVHEVFGGGLHFSRCGWPTAVGPGRHPISSAYFWYFRNPLGGMIEYFCDPDFVT
                     PRWKPTNYRLNRFSEWHLVDGIKQADDGHLRPSLAMAKAIDKSVDRGS"
     gene            complement(2582..3028)
                     /locus_tag="AC16CX_01600"
     CDS             complement(2582..3028)
                     /locus_tag="AC16CX_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3011237.1"
                     /translation="MEMTGEQLIPLTQSATWDALNDPAILKDCIPGCETITRLADNEY
                     EVVMTAKVGPVSAKFKGKMTVADAEPPRAYRLSFEGQGGVAGFAKGEAAVALTPEGNG
                     TRLAYTAKAMVGGKLAQVGARLIDGVAKQIAGKFFEAFNKRVSAKS"
     gene            complement(3019..4242)
                     /locus_tag="AC16CX_01605"
     CDS             complement(3019..4242)
                     /locus_tag="AC16CX_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3011238.1"
                     /translation="MTLLHANTGKLAENVMHFARVLRGAGLPVGPDRMLDALNALQIT
                     GVQRRDDFYWTLAAVFLGRHDQFPLYDQAFRVFWRDPQLAARVRNLPAAHGRVPPRDR
                     DPEFPARLAQALVRQRGARSSAPAGERVEFDASCTVSARESLRAIDFESMTNEELEAA
                     RAAIAELRLRLPLLPTRRHVPHARGARVDPRASLRASMKDGGATIPLRRRALRRRTPP
                     LVVLADVSGSMSRYTRMFLQFLHAATNECGRATVFTFGTRLTNITRQLRHRDADAALA
                     RVSRAVKDWSGGTRIGACLREFNVQWSRRLHGQGAIVLLISDGLDCEGADDLARQMEW
                     LHKSCRELIWLNPLLRYEGFESKPAGIKAMLPHVDAFLPVHNLQSLAGLCQALERLAI
                     RPAPRPGKERKHAWK"
     gene            complement(4313..4444)
                     /locus_tag="AC16CX_01610"
     CDS             complement(4313..4444)
                     /locus_tag="AC16CX_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR3011239.1"
                     /translation="MDENEISHAVIGAAIEVHRVLGPGLLESVYEAALSHELRFHCG"
     gene            complement(4518..5417)
                     /locus_tag="AC16CX_01615"
     CDS             complement(4518..5417)
                     /locus_tag="AC16CX_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011240.1"
                     /translation="MTALPKSIDETIALLGAHDYIAERSLATALFLALKMERPLFLEG
                     EAGVGKTEVAKVVARALNRKLVRLQCYEGLDIAAAVYEWNYQRQMVEIRLAESSGQKA
                     RPELESSLFSEKFLIERPLLQALRGGEAPPVLLIDELDRTDEPFEAYLLEVLSDWQVS
                     IPEIGTIRANRAPVVVITSNRTREIHDAVKRRCLYHWVNYPDALRELEIVRCKAPEAP
                     ERLSREVVFFVQKLRGMELFKLPGVAETIDWCKALTALDAVALDPDTVDNTLGVVLKY
                     QDDVVKVRGPEAAAILRDVKAAA"
     gene            complement(5551..>5802)
                     /locus_tag="AC16CX_01620"
     CDS             complement(5551..>5802)
                     /locus_tag="AC16CX_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011241.1"
                     /translation="GAHHDAKRGKTEEELLFSKLDSMQQIRLLDKSAIYFELLSAGQA
                     LVRSEDLNKLIAKVRPDYKVSKAAPNNGLQGDAPQASRA"
BASE COUNT         1015 a         1864 c         1862 g         1061 t
ORIGIN      
        1 cgatggcgtg ggattcccac agggtgaggc cgtcgtcccg tatcgtcggc accagcccgt
       61 tgggattcat cgctagatac tccggcgtgt tggtgacgcc gaatttccct ccggcgtcga
      121 tccgctcgta ctcgagcttc agctcggcgc agcaccagag caccttctgc acgttgatcg
      181 agttggcgcg gccccagatt ttcaacatct tcgtctccct aactgcgtaa cttggtaaag
      241 cgcgacatga aaagtcgtga catgatgcgg cgtgacatga tgcggcgtga catgatacgg
      301 cgcggttgct ctacaagcgc tgatcacgtt ctaccacgtc acgtagtacc acgtcacgaa
      361 tcaccacgtc acgcaatacc atgtcacgat ttattcagcg gtgatgttcc gctcgcgtat
      421 gatccggctc cacttcgcgg tttcgctgcg gatccacgcg ccgaattgct ccggcgtgct
      481 cccgacggtc tcgtagccca tgcccgcaaa acgctccttc tgctccggcg ccgccaggac
      541 cttgatcacc tccacgttga gccgccggat gatcgcgcgc ggtgttttcg ccggcgcgag
      601 caggccgacc caggattggg cctcgaattg cgggtaaaac tcgccgatgg aaggcgcgcc
      661 cggaacgagc gcgctgcgcc ccttcgagct tacggcgagc acgttcagac gcccggcctt
      721 cacgtgcggc agcaccagtc ccgccgaggc gatcatcgcc tcgacatggc cgccgagcag
      781 gtcgttcaac gccgggccgc cccccctgta gtgcaccagg ttggcctcga gcccgctcgc
      841 gaccttgaac aactccacgg acagccggct cgaagtggcc gcgcccgccg tcgcgaagtt
      901 gaccggcacg cgccgggact tcgcgagcgc gaggaactcg ttgaacgtcc ttgcgccgag
      961 ggcgggatgg acgacgagca gctgcgggcc gcggatcagc agggagatcg gcgcaaaatc
     1021 ccggatcgtg tcgtagggcg cattcttgaa cacgtacgga ttggatatga agctgtcgaa
     1081 caccgcgagc agggtgtggc cgtccggcgc ggccttggcc acggtatcgg tgccgatgat
     1141 cccgccggcg cccgtccggt tgtccacgac gatggactgc ccgagtgctt cggagagctg
     1201 cggcgttacg gctctcgcca cgatgtcggt cacgccgcca ggcgaggccg ggacgaccgt
     1261 ccggatcggt ctgtcgggat agttctgcgc tacggccgca gcgccgagaa gcagggccgg
     1321 ggcgatggcc caataccata tcgttcttgt ttgcatggct cgggaacggc gaaagcctga
     1381 atgttaatgg gtaatcagcg aatagtgaat tgcctaacgc ccgctgccga attaggattc
     1441 ggttggacca gttactggtc accggtcacg agtcacgcag tcgggaaaac catggcattc
     1501 accggcatcg aatcggtcgt tttcggcgcg cccgacctga agctcgcgcg caagctgttc
     1561 tccgactggg gcctggaaaa actgagcgcc ggcgagtcag gcgcggtatt cgagaccggc
     1621 atcggcggcc gggtggtggt gcgcccggag aactcgcggc gattgccgcc gaaactggcc
     1681 ggcggctcca acttccgcga gctcgtatgg ggcgtttcga gccgccggca cttcgatgcg
     1741 atagcgcaag agctcgcgcg ggacaggaag gtgagcgagg agaaggacgg ctcgatccat
     1801 gcgacggacg actgcggcat ccacatcggc ttcaggctcg gccttcgcgg caagggcagt
     1861 ccgcgccggg ccacgccgtt caattcgccg gcgcaccgcg cccgtgtgga cagggttgcc
     1921 gccgtctacg agcgcgcgcg gccgtaccgg atgggccaca tcgcgttttt tgtgcccgat
     1981 gtgaagacgg gcgagaagtt ctacatgcag cgcctcggct tctggctgtc cgaccgctac
     2041 gcgggcgggg cggctgcgtt ccttcgcaac gccgcggcga gcgaccacca caacctgttc
     2101 ctcctcaaga gccgcaccgg cgcgaccgac atccaccaca tcgcgttcga agtgtcggac
     2161 gtgcacgaag tgttcggggg cggcctgcac ttctcgcgct gcggctggcc gaccgcggtc
     2221 gggcccggcc gtcaccccat ctcgtcggcc tatttctggt acttccggaa cccgctcggc
     2281 ggcatgatcg agtacttctg cgacccggat ttcgtcacgc cccgctggaa gcccaccaac
     2341 taccgcctca accgcttctc ggaatggcat ctcgtggacg gcatcaagca ggcggatgac
     2401 ggtcacctgc ggccctcgct cgcgatggcc aaggccatcg ataaaagcgt ggatcgtgga
     2461 tcgtgattcg tcccgttcca atgtccatag cagaggtgaa cgggtggatt cttgaccgat
     2521 taccgcatgg aaatcattgg aacgggattc gtgagtcgac ccacaaccca caacccacga
     2581 ttcacgattt tgcgctgacg cgcttgttga atgcctcgaa aaattttccc gcgatctgtt
     2641 tcgccacgcc gtcgatgagc cgcgcgccga cctgcgcgag cttgccgccg accatggcct
     2701 tggcggtata ggcgagccgg gtgccgttgc cttccggcgt aagcgccacg gctgcctcgc
     2761 ccttggcgaa gcccgcgacg ccgccctggc cctcgaacga gaggcggtag gcgcgcggcg
     2821 gctcggcgtc agccaccgtc atcttcccct tgaacttggc gctgaccggg ccgactttcg
     2881 cagtcatcac cacttcgtac tcgttgtcgg cgaggcgcgt gatcgtttcg cagccgggga
     2941 tacagtcctt gaggatggcc ggatcgttga gcgcgtccca ggtcgcggac tgcgtcagcg
     3001 gtatcagctg ttcgcccgtc atttccatgc gtgctttctc tccttcccgg ggcggggcgc
     3061 gggccggatg gccaggcgct cgagcgcctg gcacagcccc gccaggcttt gcagattatg
     3121 gaccggcagg aacgcgtcca cgtgcggcag catcgccttg atgccggcgg gtttggactc
     3181 gaagccctcg tagcgcagca ggggattgag ccagatcaat tcgcggcagg atttgtgcag
     3241 ccactccatc tggcgggcaa ggtcgtcggc gccttcgcag tcgagcccgt ccgagatcag
     3301 cagcacgatc gcgccctggc cgtgcagccg gcgcgaccac tgcacgttga attccctgag
     3361 gcaggcgccg atgcgcgttc cgccggacca gtccttgacc gcgcgcgaga cgcgcgcgag
     3421 ggcggcatca gcgtcgcggt gccgcagctg gcgggtgata ttggtgagcc gcgtgccgaa
     3481 ggtgaatacg gttgcgcgtc cgcactcgtt cgtcgcggcg tgcagaaatt gcaggaacat
     3541 gcgcgtgtac cggctcatcg agcccgacac gtcggcgagc acgacgagcg gcggggtgcg
     3601 ccggcgcaat gcgcggcgtc gcagcgggat cgtggcgccg ccgtccttca tgctggcgcg
     3661 caggctcgcg cgcgggtcca cgcgcgcgcc gcgcgcgtgc ggcacatggc ggcgcgtggg
     3721 cagcaacggc agccgcagac gcagctccgc aatggcggcg cgcgccgcct ccagttcctc
     3781 gttcgtcatg ctctcgaagt cgatggcgcg cagcgactcg cgcgcagaaa cagtgcagct
     3841 cgcgtcgaat tcgacgcgct cgcccgcggg cgcggatgag cgggcgccgc gctgccggac
     3901 gagcgcctgt gcgagccgcg cggggaactc cggatcgcgg tcccgcggcg ggacgcgccc
     3961 atgcgcggcc ggcaggttgc gcacgcgcgc ggcaagctgc gggtcgcgcc agaacacgcg
     4021 aaacgcctgg tcatagaggg ggaactggtc gtgacggcca aggaataccg cggccagcgt
     4081 ccagtagaag tcatcgcggc gctggactcc ggtgatctgc agggcgttca gcgcatcgag
     4141 catgcggtcg ggaccgaccg gcagccccgc tccgcgcagc acgcgcgcga aatgcatcac
     4201 gttttccgcc agcttgcctg tgttagcgtg cagtagagtc atgcaaatct tcgacgcaaa
     4261 gccgcaaagt cgcaaagatt acaaattatt cacaatgcgt cgaacggaag cgctaaccgc
     4321 agtggaatct cagctcatgc gaaagggctg cttcgtacac ggattcgagt aagccaggcc
     4381 caaggacgcg atgcacctct attgctgcgc cgatgaccgc atgagagatt tcattttcat
     4441 ccacggtgcg ccttgggttt gacttgagat gttcgatgtg atgtctttgc ggctttgcga
     4501 ctttgcgtct atgcctgtca cgctgccgcc ttcacgtccc tgaggatggc tgccgcctcc
     4561 ggcccgcgca ccttgacgac gtcgtcctgg tacttgagca ccacgcccag cgtgttgtcc
     4621 acggtgtcgg gatcgagcgc cacggcatcg agggcggtga gcgctttgca ccagtctatg
     4681 gtctcggcca cgcccgggag cttgaaaagc tccatgccgc gcagcttctg cacgaaaaaa
     4741 accacctcgc gcgacaggcg ttccggcgcc tcgggggcct tgcagcgcac gatctccagc
     4801 tcgcgcagcg cgtccgggta gttgacccaa tggtagaggc agcggcgttt cacggcgtcg
     4861 tggatctcgc gcgtgcggtt ggaggtgatg accacgaccg gcgcgcggtt tgcccggatc
     4921 gtgccgatct ccgggatgct gacctgccag tcggatagca cctcgaggag gtaagcctcg
     4981 aacggctcgt cggtgcggtc gagttcgtct atcagcaaca ccggaggcgc ttcgccgccg
     5041 cgaagcgcct gaagcagcgg ccgttcgatc aggaactttt cggaaaagag gctggattca
     5101 agctccggcc gtgctttctg gccagagctt tcagccaacc tgatttcgac catctgacgc
     5161 tggtagttcc actcgtaaac tgcggcggca atgtcgagac cctcgtagca ctgcaggcgc
     5221 accagtttgc ggttgagcgc gcgcgcgacg accttggcca cctcggtctt gcccacgccc
     5281 gcctcgccct cgaggaacag cgggcgctcc atcttcagcg cgaggaacag ggcggtggcg
     5341 agactgcgct cggcgatgta gtcgtgcgcg ccgagcaggg cgatcgtttc gtctatcgat
     5401 ttcggcaatg cggtcacgga cgaatattaa ccgccaagac gccaagatgc caacgaaata
     5461 agatcaaagt aacatcgaac cgccaaggcc gccacgagcg ccaagaaaat caagattaaa
     5521 taggaacaat aagaacttta acgtccggct tcaggcgcgc gacgcttgcg gcgcgtcgcc
     5581 ctgcaagcca ttgttaggcg ctgccttact taccttgtag tccgggcgaa cctttgcgat
     5641 aagcttgttt aagtcttccg aacgcacgag cgcctgaccg gcagatagga gttcgaagta
     5701 gatcgccgac ttatccaaga gccggatttg ctgcatgcta tccaacttcg agaacagtaa
     5761 ctcctcctct gtttttcctc tctttgcgtc atgatgcgcg cc
//
LOCUS       DBNGWZ010000054         1534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_4635, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000054 DBNGWZ010000000
VERSION     DBNGWZ010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_4635"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CX_01625"
     CDS             complement(<1..386)
                     /locus_tag="AC16CX_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011242.1"
                     /translation="MNLARQLAERVTATRYEDLTPEAVYWCRIAVLDTLGVMLAGSVE
                     EAPRIVEDVLGLQSGDGPCLLFGTRRRVRPLDAALVNGTAAHALDFDNTAKNLGGHAS
                     AVMVPALIAAGEAFGASGRDLILAHAA"
     gene            complement(394..1356)
                     /locus_tag="AC16CX_01630"
     CDS             complement(394..1356)
                     /locus_tag="AC16CX_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011243.1"
                     /translation="MNIMLPLVAAALTSVVAPGAWAQGYPSKPIRLISPFAPGGGTDI
                     LGRLIGPQVSETLGQPIVVENRPGGGGTVGAGITVRSEPDGYTLIVVSGSYGANAALY
                     NLPYDSVTDIQPIILIGTTGLLVSMHPSVPIKTIKELIAYARANPGKLNFGSAGTGGL
                     GHLAGELFKLDTKVVFTHVPYKGSGPVMTALLSGEVDSSFSSLVPSMPHVKAGRLRAI
                     ALTTPKRSRALPEVPTVGETVPGYDVTHWYGMWGPKGLPREIITRWNQEVARVLRTDA
                     MQKWLAQEGLEAAGGPPDEFRDRIRNDVEKWKKVVKEAKIVISN"
BASE COUNT          259 a          510 c          493 g          272 t
ORIGIN      
        1 gcggcgtgcg ccaggatcag gtcgcgcccg ctggcgccga atgcctcgcc cgcggcaatc
       61 agcgcgggga ccatcaccgc cgacgcgtgc ccgcccagat tcttggccgt attgtcgaag
      121 tccagcgcgt gcgccgccgt gccattgacg agcgcagcgt cgagcggacg cacgcgccgg
      181 cgggtgccga agaggaggca cggtccgtcg ccggactgaa gccccagcac gtcctcgacg
      241 atgcggggcg cctcctcgac ggaaccggcc agcatgacgc ccagcgtgtc gagcacggcg
      301 atcctgcacc agtagacggc ctccggcgtg aggtcttcgt aacgcgtagc ggtgacgcgc
      361 tccgccaatt ggcgtgccag gttcatgcga ggatcaattc gagatgacga tttttgcctc
      421 tttgacgacc ttcttccatt tttccacgtc gttcctgatc cggtcgcgaa attcatccgg
      481 cgggccgccg gcggcttcca gtccctcttg cgccagccac ttctgcatgg cgtcggtgcg
      541 cagcactctc gcgacttcct ggttccaccg cgtgatgatt tccctcggca agcccttggg
      601 tccccacatg ccgtaccagt gagtcacatc gtaaccaggc accgtttcgc cgacggtggg
      661 cacctccggc agcgcgcgcg agcgcttggg cgtggtcaag gcgatggcgc gcaaccgccc
      721 cgccttcacg tgcggcatgc tcgggaccag actgctgaag ctcgaatcca cttccccgct
      781 gaggagggcg gtcatgaccg gaccgctgcc tttgtacgga acgtgggtga acaccacctt
      841 ggtgtccagc ttgaaaagct cgcccgcaag gtggccgagt ccgccggtgc cggccgagcc
      901 gaagttgagc ttgccggggt tcgccctggc ataagcgatg agctccttga tggtcttgat
      961 cgggaccgac gggtgcatcg atacgagcag cccggttgtt ccgatcagga taatcggctg
     1021 aatgtcggtg actgaatcat acggcaggtt atagagggcg gcgttggcgc cgtagctgcc
     1081 cgagaccacg atcagcgtgt agccgtcggg ctcggagcgc accgtgatcc cggcgcccac
     1141 ggtaccgccg ccaccgggcc ggttctcgac cacgatcggc tgcccgagag tctcggacac
     1201 ctgcggcccg atgaggcgcc ccagaatgtc ggtgcctcca ccgggagcga aggggctgat
     1261 caaccggatc ggcttggatg gatacccctg cgcccatgcg cccggggcga cgacggacgt
     1321 gagtgccgcg gcaaccagcg gcagcatgat attcatcttc ccctcctcga aaaaagctcg
     1381 cggcctgccg cgcctggagc tttgatcaaa gttcacagcg gagccgttca tgattcgaca
     1441 agctcacaac gaacggcgct tacccggcct tgcccgtcga ggcgtttcca gggtttcgtc
     1501 ggacggggac gaccatacca tacggacgtt gaag
//
LOCUS       DBNGWZ010000055         5690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_4675, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000055 DBNGWZ010000000
VERSION     DBNGWZ010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_4675"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="AC16CX_01635"
     CDS             <1..876
                     /locus_tag="AC16CX_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011244.1"
                     /translation="KRIRWDIDRDVIRGRTFDFSRKFLPDGLSLVDKLDFLDANERRL
                     LSQVQGRTYANIFGLVERYIGAKILEISRDHWLGDQTALEALVRFTDEELKHQELFRK
                     IERMVAEGMPPGYRFLPQPNEVASVVLGKSTWAVLALTAHIELFTQVHYRQSIAPDEN
                     LSELYQDVFLFHWKEESQHAILDELEWQREDDKLTAEERDRAVGDLIGLVAAVDGILQ
                     VQAPADVEYFVAACGRRFTPEEMERLHAGVLKAYRWQYIVSGVKDPRFNELLGRMITA
                     EQSRRIGEALGPIMH"
     gene            complement(998..1975)
                     /locus_tag="AC16CX_01640"
     CDS             complement(998..1975)
                     /locus_tag="AC16CX_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011245.1"
                     /translation="MLDSARPSPRFIAAVAALAVACSVCAQGYPEKPVTLVVPFPAGG
                     ATDPVTRAIGGRMSEIWKQPVVVLNRAGAGGNIGAESVARAAADGYTLLIGTTAHAIG
                     PSLYAKLGYDVLKDLAPVSQLTISPNLLVVHPSLPVQSVKALVALAKARPGQLISASA
                     GTGTSNHLSLLLLTSLAGIDIVHVPYKGAAPAVADVVGGHAHMTFAPAPASLALVQAS
                     RLRLLAVTTARRWSVLPDVPTVAEGGVPGYELTSWVGLLAPSATPQPVIARIHAAVVE
                     SLRTPAVRDILVKSGTEPLGNTPQEFAQALRSEIAKYSRTIKAAGIKTD"
     gene            complement(1968..2462)
                     /locus_tag="AC16CX_01645"
     CDS             complement(1968..2462)
                     /locus_tag="AC16CX_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3011246.1"
                     /translation="MAKIPSKKRSTAGKPQSAQPQGVKAGGGRYIFDLATLDPIEAGP
                     GYSTAAGSLVEGERMMIGLMRMPRGTGALPHSHPNEQWVYVLEGTLKVDIDGNKRDAA
                     AGSLVFIPANAVHALVASADGDAVFLTAKDTSHGIAGTAVGDSSAGAHYEPGFGPAER
                     RKNA"
     gene            2628..3758
                     /locus_tag="AC16CX_01650"
     CDS             2628..3758
                     /locus_tag="AC16CX_01650"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011247.1"
                     /translation="MQLKAEGGNHQAEGLRVYFIPHPWSSTPACRAASRRAFAGSNVR
                     RHVLRNPGSPLIPRLSAFILGCALAAGAAIGQEFPSNPVRFVVPLPAGGTMDIIARTL
                     GAPVSRALGQNVIVDNRPGGGGGGIIGTEAVARAPADGHTVLFIGNGFTMNPALRSKL
                     PYDTVKDFTAVARVASNPLAISVHPSLPVRTLKELVAFARARPGELTFASGTATGGLR
                     LNAERFKAAAGIDIVNVPYQGGVPAVTAVIGGHTGILIVNVSETVPHVQSGKLRALAV
                     TSLARADVLKDVPTVAESGYPGFEALMWYGVVARSAVPKGAIDRLNGEIARALQLPEV
                     AETLGRLGLARASMGPAAFDAFIRSEIESNGKLIRKLNLRVE"
     gene            3948..>5690
                     /locus_tag="AC16CX_01655"
     CDS             3948..>5690
                     /locus_tag="AC16CX_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3011248.1"
                     /translation="MTSSKRASSAIVHDTPVPARESEEFWGSDPIAAMLRELGVPYIS
                     LVPGSSYRGLHDSIVNYLGNRMPQIVLCIHEENAIAVAHGYAKVTDRMMAAAVHANIG
                     LLRTPMAVYNAWCDRAPVLIMGATGPWDATKRRPWIDWIHTSADQGGLIRNFTKWDNQ
                     PGSPEAATEALLRAAQIAQTAPKGPVYLNLEVEMQEKKLDAMPVLPDPARYPVPKAAR
                     PDPGLVAAAAKLLSHARNPVILAGRCSRSLEGWNARVALAEKLNAPVLTSIKIAAAFP
                     TDHRLHPVPPFQQLLPEAKKLIAAADVILSLDWLDLAGTLKQTYGSSDAVRAKVIQVS
                     CDAYSHRGWSMDHQGLPPMDIYMMCETDAAVPLLLDAVKPRMAGAVLPERVPLPPIPG
                     EVSLRGVGAALETATRGIDVCYTRLPLGWSGAYTHFRHPLDYLGHDGGAGVGSGPAVT
                     IGAGLALKGSGRIPIALMGDGDFLMGNTAVWTAVHYQIPCLMIVCNNRSFYNDERHQG
                     RMARARGRPEENKWIGQHLTNPDINIAGMARMMGAEGIGPVDKVAEVLPALERGIAAV
                     REGKVCAIDARVLPG"
BASE COUNT          962 a         1866 c         1961 g          901 t
ORIGIN      
        1 aagcgcatcc gctgggacat agaccgcgac gtgatccgcg gccgcacgtt cgatttctcg
       61 aggaaattcc tgcctgacgg gctgtccctt gtggataagc tcgactttct cgacgcgaac
      121 gagcgccggc tgctgagcca ggtccagggg cgcacctacg ccaacatctt cggcctggtg
      181 gagcgctaca tcggcgccaa gatcctggag atcagccgcg atcactggct gggcgaccag
      241 accgcgctcg aggcgctggt gcgcttcacc gatgaggagc tcaagcacca ggagctgttc
      301 cggaagatcg agcggatggt cgccgaaggg atgccgccgg gataccgctt cctgccgcaa
      361 ccgaacgagg tcgcgtcggt ggtgctcggg aagtccacct gggcggtgct ggctttgact
      421 gcccatatcg agctgttcac ccaggtccac taccgccaga gcatcgcgcc ggacgagaac
      481 ctgtccgagc tctaccagga cgtgttcctg ttccattgga aggaggagtc ccagcacgcg
      541 atcctcgacg agctggaatg gcagcgcgag gacgacaagc tcaccgccga agagcgcgac
      601 cgcgcggtcg gcgacctgat cgggctggtc gcggcggtgg acggcatact ccaggtgcag
      661 gcgccggctg acgtcgagta tttcgtcgcg gcctgcggcc ggcggttcac gccggaagag
      721 atggagcgcc tgcacgccgg ggtgctgaag gcttaccgct ggcagtacat cgtttcgggc
      781 gtgaaggatc cgcgtttcaa cgaattactc ggcaggatga tcacggcgga gcaatcgcgg
      841 cgcatcggcg aggcgctcgg cccgatcatg cactgaacag cgctcgcggg tgacgagtgt
      901 cgggtgtcga gtgtcgggtg tcgagtggta gccgccctct tacggtctcg cccctcgaca
      961 ctcaatcctc gacactcttt tctcatccct cgcggactca gtccgttttg atgcccgccg
     1021 ccttgatcgt cctgctgtac ttggcgatct cgctcctcaa cgcctgcgcg aattcctgcg
     1081 gcgtgttgcc gagcggctcg gtgccggact tgactaggat gtccctgacg gccggcgtgc
     1141 gcaggctttc gacgacggcg gcgtggatgc gtgcgatgac cggctgcggt gtggcggacg
     1201 gcgcaagcag ccccacccag gaagtgagct cgtagccggg tacgccgccc tccgcgacgg
     1261 tgggcacgtc cgggagcacc gaccagcgcc gggccgtcgt gaccgcgagc aggcgcaagc
     1321 ggctcgcctg caccagcgcc agcgacgccg gcgcgggggc aaacgtcatg tgcgcgtggc
     1381 cgccgacgac gtcggcgacg gcgggcgcag cgcccttgta cggcacgtgg acgatgtcaa
     1441 tcccggcgag actggtaagc aacagcaacg aaaggtggtt cgaggtgccg gtccccgccg
     1501 aagccgaaat caattgcccc ggcctcgcct tggcgagcgc gaccagcgct ttcactgact
     1561 ggactggaag cgacggatgc acgaccagca gattcggcga gatcgtgagc tggcttaccg
     1621 gagcgaggtc cttcagcacg tcgtagccca gtttggcgta gagactcggc ccgatcgcgt
     1681 gcgccgtcgt gccgatgagc agcgtatagc cgtcggccgc ggcgcgggcg accgattccg
     1741 cgccgatgtt gccgccggcg cccgcccgat tgagcaccac gaccggctgc ttccagatct
     1801 cgctcatgcg gccaccgatc gcgcgcgtga cgggatcggt cgcacctccc gccgggaacg
     1861 gcacgaccag cgtgaccggc ttttcggggt agccctgcgc gcacacgctg caggcgacgg
     1921 ccagggcggc gacggcggca atgaatctcg gtgagggccg agcggagtca agcattttta
     1981 cgacgctcgg caggcccgaa gccgggctcg taatgcgcgc cggcggacga atcgccgacg
     2041 gcggttcccg cgatcccgtg cgaggtgtcc ttggcggtca ggaacacggc gtccccgtcg
     2101 gcggacgcca ccaacgcgtg cacggcgttg gcggggatga agacgagcga gcccgcggct
     2161 gcgtcccgct tgttgccatc gatgtcgacc ttcagcgtgc cttcgagcac gtagacccac
     2221 tgctcgttgg gatggctatg gggcagggcg ccggtgcccc gcggcatccg catcaggccg
     2281 atcatcatgc gctcgccctc gaccagcgat ccggcggcgg tcgaataacc cgggcctgct
     2341 tcgatcgggt ccagcgtggc gagatcgaag atataccgcc cgccgcccgc cttgacgccc
     2401 tgcggctgag cggattgcgg cttgccggcc gtggagcgct tctttgacgg tatcttcgcc
     2461 atgacccatg aatgaatcgg ttacagcata acggactata ttagccacaa atagttgaat
     2521 cggcacgagc agtacggaac gaaaacggcg gcggcacgat tgatttatca tccagccagg
     2581 agtttgtcgg agccgattcc ggcaagctcc ttgggagtgg aggagagatg cagctcaagg
     2641 cagagggcgg aaatcatcag gcagaaggat tacgggttta tttcatccct catccctggt
     2701 cttcgacccc ggcctgccgc gctgcatcgc gtcgggcgtt cgccggctcg aacgtgcgca
     2761 ggcacgttct tcgaaacccc ggctcacccc tcatccctcg gttatccgcc ttcatccttg
     2821 gctgcgcgct ggcagccggg gccgccattg ggcaggaatt cccgtcgaat ccggtgcggt
     2881 tcgtcgtccc gttgccggct ggcggcacca tggacatcat cgcgcgcacg ctgggagcgc
     2941 cggtcagccg ggcgctcggc cagaacgtga tcgtggacaa ccggcccgga ggcggcgggg
     3001 gcggcatcat cggtaccgag gcggtggcgc gcgcgccggc ggacgggcat acggtgctct
     3061 tcatcggcaa cggcttcacg atgaatccgg cattgcggtc gaagctgccg tacgacacgg
     3121 tgaaggactt caccgcggtc gcgcgggtcg cttcgaaccc gctggcgatc tccgtccacc
     3181 cctcgctgcc ggtgaggacg ctcaaggagc tggtggcgtt tgcgcgcgcc cgtccgggcg
     3241 agctcacgtt cgcgtcgggc accgcaacgg gcggactgcg gctcaacgcg gagcgcttca
     3301 aggcggcggc gggaatcgat atcgtcaacg tgccctacca aggaggtgtg ccggcggtga
     3361 ccgccgtgat cggcgggcac accggcatcc tgatcgtgaa tgtttccgag acggtgccgc
     3421 acgtccagtc gggcaagctg cgcgcgctcg ccgtgacttc gctcgcgcgc gccgacgtgc
     3481 tcaaggatgt cccgaccgtc gccgaatccg gctatccggg attcgaggcc ctcatgtggt
     3541 acggcgtggt ggcgcgctcg gcggtgccga aaggcgcgat cgaccgtctg aacggcgaga
     3601 tcgcgcgggc gctgcagctc cccgaagtgg ccgaaaccct cggcaggctg ggcctggcgc
     3661 gggcgtccat ggggcctgcg gcgttcgatg cgttcatacg ctccgaaatc gagagcaacg
     3721 gaaagctcat ccggaaactg aatctccggg tcgagtgacg agtgtcgagt gtcgagtgtc
     3781 gagtgacgag ggacgaggga cgagggacga gtgtcgagtg acgagatgcg ggatgcgaga
     3841 tgcgcgatgc gggtggggta atataacgac taacgcccaa tcgaatttcg gatcccggat
     3901 cacgcatcac ggatcgcgat tcacgcaacc cgacggaacc caaaccgatg acttcgagca
     3961 aacgtgcttc cagcgccatc gtccacgaca cgccggtgcc ggcgcgggaa agcgaggaat
     4021 tctggggcag cgatcccatc gccgcgatgt tgcgcgagct cggggttccc tatatctcgc
     4081 tcgtgccggg ctcgagctac cggggcctcc acgacagcat cgtcaactat ctcggcaacc
     4141 ggatgccgca gatcgtgctc tgcatccacg aggagaacgc gatcgcggtg gcgcacggct
     4201 acgcgaaggt caccgaccgc atgatggccg cggcggtgca cgccaacatc gggctgctgc
     4261 gcacgccgat ggcggtctat aacgcgtggt gcgaccgcgc gccggtgctg atcatgggcg
     4321 cgaccgggcc ttgggacgcc acgaaacgcc gcccctggat agattggatc cacaccagcg
     4381 ccgaccaggg cgggctgatc cgcaatttca caaaatggga caaccagccc ggttcgccgg
     4441 aagcggcgac ggaagcgctg ctgcgcgccg cgcagatcgc gcagaccgcg ccgaagggcc
     4501 cggtctacct gaacctggaa gtcgagatgc aggagaagaa gctcgacgcg atgccggtgc
     4561 tgcccgaccc ggcgcgctac ccggtgccga aggcggcgcg ccccgacccc gggctggtcg
     4621 ccgccgccgc gaagctgctg tcgcacgcca ggaacccggt catcctcgcc ggccgctgct
     4681 cgcgcagcct ggaaggctgg aatgcgcgcg tcgcgctggc cgagaagctg aacgcgccgg
     4741 tgttgaccag catcaaaatt gcggcggcgt ttccgaccga ccatcgcctg cacccggtgc
     4801 cgccgttcca gcagctcctg cccgaagcga agaagctcat cgccgcagcc gacgtgatcc
     4861 tgtcgcttga ctggctcgac ctcgccggca cgctcaagca gacctacggc agcagcgacg
     4921 ccgttcgcgc gaaagtgatc caggtctcgt gtgacgccta cagccaccgc ggctggagca
     4981 tggaccacca gggcctgccg ccgatggata tctacatgat gtgcgagacc gacgccgcgg
     5041 tgccgctgct gctcgacgcg gtcaagccgc gcatggcggg cgcggtgctg cccgagcgcg
     5101 tgccgctgcc gccgatcccc ggcgaagttt cgctgcgcgg cgtgggcgcg gcgctggaaa
     5161 ccgccacgcg cgggatcgac gtctgctaca cgcggctgcc gctcggctgg agcggcgcct
     5221 acacccactt ccgccacccg ctcgactacc tcgggcacga cggcggcgcc ggcgtgggct
     5281 cggggccggc cgtgacgatc ggcgcgggcc tcgcgttgaa ggggagcggc cgcattccga
     5341 tcgcgttgat gggcgacggc gatttcctga tggggaacac ggcggtctgg acggccgtgc
     5401 actaccagat cccgtgcctg atgatcgtgt gcaacaaccg ctccttctac aacgacgagc
     5461 gccaccaggg gcgcatggcc agggcgcgcg gccggccgga agaaaacaag tggatcggcc
     5521 agcacctcac caacccggat atcaacatcg cgggcatggc gcgcatgatg ggcgccgagg
     5581 gcattggccc ggtggacaag gtcgctgaag tgctgccggc gctcgagcgc ggcatcgcgg
     5641 cggtccgcga aggcaaggtc tgcgcgatcg acgcgcgcgt gctgcccggc
//
LOCUS       DBNGWZ010000056         2812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3027862, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000056 DBNGWZ010000000
VERSION     DBNGWZ010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3027862"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..553
                     /locus_tag="AC16CX_01660"
     CDS             83..553
                     /locus_tag="AC16CX_01660"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3011249.1"
                     /translation="MLTIAIPGFRTLQLEHLVLDYNGTLARDGVLIEGVADRLRRLAT
                     SLSLHVVTADTFGKARQALAGLPCELTILPVENQDAGKLACIKRLGAQHCVCIGNGRN
                     DRSMLKAAGLGIAVVQTEGAAAETVVVADVISPGILDALDLLAHPLRLVATLRS"
     gene            951..1955
                     /locus_tag="AC16CX_01665"
     CDS             951..1955
                     /locus_tag="AC16CX_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011250.1"
                     /translation="MEFQRTTPLHLGIAMVLSVVAGATTDAVRAQPSWRPEKTVEIVV
                     PTGAGGINDQIARLMQRVLQEGKLVPTPIIILNKAGGNQSLAVVYLTQKAPDPHSLLY
                     ATATVFTNQLAGLTPLGYSDLTPLACLLVDYTVITVAADSPVKTMRDLVERLKVDSGA
                     ISFGMVSRGGPNHLALSQAVRSAGIDPRKLKTVVFKTNAESHMAVVGGHIQAVVSSVS
                     AALPQVQAGHARMLAIAAPQRMTGPLAGLPTMREQGINATGISNWRAIFGAKGMTPAQ
                     AAFWDGALARMAASEEWDRELDGTNMARLFLRGRDFARYLDEEYAATRAVMTDLGLIK
                     "
     gene            1972..>2812
                     /locus_tag="AC16CX_01670"
     CDS             1972..>2812
                     /locus_tag="AC16CX_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3011251.1"
                     /translation="MAVTLGYQALQGALSMSDAIDLLERAFAHETAGRAYLSPKFVSN
                     FEGGSMRMLFAADYEAGYSATKIYQNVRGAGARFLVSLYRLKDGELLALLDGRHITDM
                     RTGAASGVIARKVDVPGPVTVGMIGSGHQARAQLESLASVYGINRAVVYSPTRANREA
                     FAREMSAKLGFAVETVESAQAAVRGHPVVAAASSARGAEPVLRGEWLEQCRLLCAVGN
                     TRAQFAEVDVRCFRDARLVVMDTPHALEEAGELRRAVEAGALPEAKRATLGQIVTGAV
                     TVPK"
BASE COUNT          500 a          859 c          965 g          488 t
ORIGIN      
        1 ctcggtgacg aggaacgtga ttcactatgc ccactgtgcg gttaccgtag tgcgctaacg
       61 caagccggta ccgtttccgg cgatgctcac gatcgcgatc cccggatttc gcactttgca
      121 gctcgagcac ctggtgctcg actataacgg cacgctggcc cgcgatggcg tgctgattga
      181 gggtgtcgca gatcgcctga ggcgccttgc aaccagcctc tcgctccatg tggtcaccgc
      241 cgatacgttc ggtaaagcgc gccaagcgct agccggcctg ccgtgcgagc ttacgatatt
      301 gccggtggaa aatcaggatg ccggaaagct cgcttgtatc aagaggcttg gcgcgcagca
      361 ctgtgtctgc atcggcaacg ggcgcaacga ccgctcgatg ctgaaggctg ctgggctcgg
      421 gatcgccgtg gtgcaaacgg agggcgccgc ggccgagacg gtggtggtgg cggacgtgat
      481 ctcgccgggc atactcgatg cgcttgatct gctcgctcat ccactgcggc tcgttgccac
      541 gttacgctcg tagactgatc cacttcatgg agctgttgtt tccaaaattc atcatctgtc
      601 actcgtgcgg cgggcggcgc cgcgtgacga ggaaagagat tttcgaaatg cccgagggcg
      661 ttgccggcat agcctgggcg aggtgccgga aatgccatca cacttttgtc cgtttcatcg
      721 gggataaaag gccggcgggc aaactgatgg aaagatggct cgatgttcac tgagaatgac
      781 cgcatgggcg tgagaagggc atcgggtctt gcaccaacgt gcgctaatgc aggacctgat
      841 tccttcatcc gaggctgatg gtcgcgcgcc acatcaacga cgcatcagac cttctgctat
      901 gcttcgtaac tttagacaag ccgtctgcga agagttaaag aggagatcgc ttggaatttc
      961 agcgcaccac gccgttacat ctgggcattg caatggtctt gtccgtcgtt gcaggcgcga
     1021 caacggacgc tgtgcgcgcg cagccgtcgt ggcgtcccga gaagacggtg gagatcgtcg
     1081 taccgaccgg ggcgggcggc atcaatgacc agatcgcgcg gctcatgcag cgcgtgctgc
     1141 aggaagggaa gctagtgccg acgccgatca ttatcctgaa caaggcgggc gggaaccaaa
     1201 gccttgcggt cgtgtatctc acccagaagg cacccgaccc gcattccctg ctgtacgcga
     1261 cggcgacggt gttcacgaac cagctcgcgg gcctgacgcc gctcggatat agcgacctga
     1321 cgccgctcgc gtgcctgctc gttgactaca ccgtcatcac cgtggcggcc gattcgccgg
     1381 tcaaaacgat gcgggacctt gtcgagcgcc tcaaggtgga ctccggcgcg atctccttcg
     1441 gcatggtgag ccgcggcggg cccaaccacc tcgcgctgtc ccaggccgtc cgctcggccg
     1501 ggatcgatcc gcgcaagctc aagacggtgg tgttcaagac caatgccgaa tcccacatgg
     1561 cggtggtcgg cgggcatatc caggcggtgg tttcgtcggt gagcgccgcg ctgccgcagg
     1621 tgcaggccgg gcacgcgcgc atgctcgcca tcgccgcgcc gcagcgcatg accggcccgc
     1681 tggcgggcct cccgacgatg cgcgagcaag ggatcaacgc caccggcata tcgaactggc
     1741 gcgcgatctt cggggcgaag ggcatgacgc ccgcgcaggc cgcgttctgg gacggcgcgc
     1801 tcgcgcggat ggcggcgagc gaggaatggg accgcgagct cgacggcacc aacatggcgc
     1861 gccttttcct gcgtgggcgg gacttcgcca gatacctgga tgaggaatat gccgccacgc
     1921 gcgcagtcat gaccgacctc ggactgatca agtagccaat ccggacattg catggccgtc
     1981 acgctggggt atcaagcgct gcagggcgcg cttagcatga gcgacgcgat cgatctcctt
     2041 gagcgcgctt tcgcgcacga gaccgcgggc cgggcgtacc tgtcgccgaa gttcgtcagc
     2101 aatttcgaag gtggctcgat gcgcatgctg ttcgccgccg attacgaagc gggctacagc
     2161 gcgaccaaga tttaccagaa cgtccggggc gccggcgcgc gcttcctcgt gtcgctctac
     2221 cgtctgaagg acggggagct gctcgcgctg ctcgacgggc gccacatcac cgacatgcgc
     2281 accggggccg cgagcggggt catcgcgcgc aaggtggatg tgccgggtcc ggtgacggtc
     2341 ggcatgatcg gctcaggcca ccaggcacga gcgcagctcg agagcctcgc ctcggtgtac
     2401 gggataaatc gcgccgtcgt ctacagcccg acgcgggcca accgcgaggc gtttgcgcgg
     2461 gaaatgtcgg cgaagctcgg gttcgcagtc gagacggtgg agtcggcgca agcggcggtg
     2521 cgcggccatc ccgtggtggc tgcggcgagc agcgcgcgcg gcgccgaacc ggtactgcgc
     2581 ggggagtggc tggaacaatg ccggctgctg tgcgcggtcg gcaacacccg ggcgcaattc
     2641 gccgaggtgg acgtgcgctg tttccgggat gcgcggctgg tggtgatgga tacgccgcat
     2701 gcgttggaag aagcggggga gctgcgacgg gcagtcgagg ccggtgccct cccggaagcg
     2761 aaacgggcaa cgctgggaca gattgtcacc ggcgccgtaa ccgtgccgaa gg
//
LOCUS       DBNGWZ010000057         2632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_256658, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000057 DBNGWZ010000000
VERSION     DBNGWZ010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_256658"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="sufD"
                     /locus_tag="AC16CX_01675"
     CDS             <1..1140
                     /gene="sufD"
                     /locus_tag="AC16CX_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3011252.1"
                     /translation="APRLAMADISDFIAPEATVRLVFVDGVFAPELSASTGLPYGVVA
                     THLMAALDTHAAVIEPHLARRAEFEREVFAALNTAYLRDGAFIWIARNQKCPTPVQLL
                     YVSTQKESAAYPRCLVVAEEAAECTLIEDYVGLGEAVYFNDAVTEIVVGEGARVRHVR
                     VQREASTAFHFATCAVAIAKDAHYASHAITLGARLSRFNLNVAQRGEGSEVLMDGLAL
                     ISGRQLADTHTLMDHASANGRCRQLHKCIVGGAAHAVFNGKIMVRPGAQLTDSAQQSR
                     NLLLSDKAHVDTKPQLEIFADDVKCAHGATVGQLDVEQLFYLRSRGLPAARARNLLTY
                     AFAAEVIDRIPVPSLVEQLEETVLAQTQTPTPSLPPSGGGQGEGG"
     gene            1143..2402
                     /locus_tag="AC16CX_01680"
     CDS             1143..2402
                     /locus_tag="AC16CX_01680"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIR3011253.1"
                     /translation="MNALHTPLRRSGADGLDVERIREDFPILRLKVNGRPLVFLDNAA
                     SSQMPRQVLDRWTRYQTTQHANIHRAVHHLSEVATAEYEGARKKLAAFINAREEREVI
                     FTTGTTDAINLVAHGYGRKFIGAGDEIVLTTLEHHSNIVPWQMVAEEKGARIRVVPVN
                     DRGELIFEEYLKLLGPRTKLVGVGHVSNALGTLNPVREMIRAAHEKGIPVLVDGAQAA
                     PHLRLDMQALDCDFYAFSGHKMFGPTGIGVLYGKAAWLEKMQPYKGGGDMILSVTFEK
                     TTYNVIPGKFEAGTPPIAAAITLGAAVDYLNGIGFERIHAHETDLLDYATDQVNRLPG
                     VRIIGTAEKKAAVLSFIVDGVHPHDVGTLLNEEGVAIRTGHHCAQPVMQRFGIPATSR
                     ASFAFYNTRAEVDALVAGIRKVQKVFG"
     gene            2452..>2632
                     /locus_tag="AC16CX_01685"
     CDS             2452..>2632
                     /locus_tag="AC16CX_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011254.1"
                     /translation="MIRVDGSAGLEYLSPITYHLSPITYHLSLITHLTAMADTKQLYQ
                     EVILDHNRKPRNWGVL"
BASE COUNT          503 a          848 c          851 g          430 t
ORIGIN      
        1 gcgccgcggc tggcgatggc ggatatttcg gacttcatcg cgccggaggc gacggtccgt
       61 ctcgtcttcg tggacggcgt gttcgcgccg gagctctcgg ccagcaccgg cctgccgtac
      121 ggagtcgtcg ccacgcatct catggcggcg ctcgacaccc atgcggcggt catcgagccc
      181 cacctcgcgc gccgcgcgga attcgagcgc gaggtattcg cggcactgaa taccgcttat
      241 ctccgcgacg gcgctttcat ctggatcgcg cgcaaccaga aatgcccgac gccggtgcag
      301 ttgctctacg tttcgaccca gaaggaatcg gcggcctacc cacgctgcct ggtcgtcgcg
      361 gaggaagccg ccgaatgcac gctgatcgag gattacgtcg gcctaggtga agcggtgtat
      421 ttcaacgacg cggtgaccga gatcgtggtg ggcgagggcg cccgcgtgcg gcacgtgaga
      481 gtgcagcgag aggcgagcac cgcatttcat ttcgctactt gtgcggtggc catcgccaag
      541 gacgcgcatt atgcatcgca tgcgatcacg ctcggtgccc gcctatcaag attcaatctc
      601 aacgtggcgc agcggggaga aggctcggaa gtcctgatgg acgggcttgc gctcatttcg
      661 ggccgccagc tcgccgacac ccacaccctg atggaccacg cgagcgcgaa cggccgttgc
      721 cggcagctgc acaagtgcat cgtcggcggc gcggcgcacg cggtgttcaa cggcaagatc
      781 atggtgcggc ctggcgccca gttgaccgac tcggcgcagc aaagccgcaa cctgttgctc
      841 tcggacaagg cgcacgtgga caccaagcca cagctcgaga tcttcgccga cgacgtgaag
      901 tgcgcgcacg gtgcgacggt gggccagctc gacgtcgagc agctgttcta cctgaggagc
      961 cgcggcctgc cggcggcgcg cgcccgcaat ctcctgacct atgcgttcgc cgccgaggtg
     1021 atagaccgca tcccggtgcc atcgctggtc gagcagctgg aggagacggt gctcgcccag
     1081 acccagaccc ccaccccatc cctccccccg tcagggggag ggcagggaga gggtggttaa
     1141 tcatgaacgc gctccacacc cctctcaggc gctccggtgc cgacggcctg gacgtcgagc
     1201 gcatccgcga ggactttccg atcctgcggc tcaaggtcaa cggaagaccg ctggtattcc
     1261 tcgacaacgc cgcctcgagc cagatgccgc ggcaggtgct ggaccgctgg acgcgttacc
     1321 agaccaccca gcacgccaat atccaccgcg cggtgcacca cctgtcggag gtcgccacgg
     1381 cggagtacga aggggcgcgg aagaagctcg ccgccttcat caacgcgcga gaggagcgcg
     1441 aggtgatctt caccaccggc acgaccgacg ccatcaatct ggtcgcgcac ggctacggga
     1501 ggaagttcat cggggcgggc gacgagatcg tgctgaccac cctcgagcac cattccaaca
     1561 tcgtgccgtg gcagatggtg gcggaggaga agggcgcgag gatccgcgtg gtgccggtca
     1621 acgaccgcgg cgagctgatt ttcgaggagt acctgaagct gctcggcccg cgcacgaagc
     1681 tcgtcggcgt gggccacgtc tccaacgcgc tcggcaccct caacccggtg cgcgagatga
     1741 tccgggccgc gcacgaaaaa ggcatcccgg tgctggtgga cggcgcgcag gccgcgccgc
     1801 acctgagact cgacatgcag gcgctcgatt gcgacttcta cgcgttctcg ggccacaaga
     1861 tgtttgggcc gacgggcatc ggcgtgctct acggaaaggc ggcgtggctc gagaagatgc
     1921 agccatacaa gggcggcgga gacatgattc tctcggtcac gttcgagaag acgacctaca
     1981 acgtcattcc gggcaagttc gaagccggca cgcccccgat cgcggccgcg attacattgg
     2041 gcgcggcggt ggactatctc aatggcattg gcttcgagcg cattcacgca cacgagaccg
     2101 atctgctcga ttacgcgacc gaccaggtga accggctgcc gggcgtgcgc atcatcggca
     2161 cggcggaaaa aaaggcggcg gtgctctcct tcatcgtgga cggcgtgcac ccgcacgatg
     2221 tcggcacgct cttgaacgag gagggcgtcg cgatccgcac cggccaccac tgtgcccagc
     2281 cggtgatgca gcgcttcggc attcccgcca cctcgcgcgc gtcattcgcc ttttacaaca
     2341 cgcgcgcgga agtggacgcc ctcgtcgccg gcatcaggaa ggttcagaag gtgtttggat
     2401 gaagcgtgat aggtgataag ggaaagatct gaaaggcgtg ataagtgaca ggtgataaga
     2461 gttgacggca gcgccggcct tgaataccta tcacctatca cctatcacct atcacctatc
     2521 acctatcacc tatcacttat cacgcatttg acggcaatgg cggatacgaa acagctttat
     2581 caggaagtga tcctcgacca caaccgcaag ccgcgcaact ggggcgtgct gg
//
LOCUS       DBNGWZ010000058         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_256729, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000058 DBNGWZ010000000
VERSION     DBNGWZ010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_256729"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          653 a         1027 c         1108 g          572 t
ORIGIN      
        1 cgggcgcggc ggggagcgtc gtcgcgagat tgacgatgcc gttcatcagc atcagcccga
       61 agaacccgcc cgtgtagccc gtgaaatcga aggcgtgcat cacgaaccag tatttgcccg
      121 tctccaggag ccagatgacg accgaggtcg cgaagaccat gaagatgctg cggggcgagc
      181 gcagagactc cagccccgtc caaaaactca ccaacatgcc ggtgatcctg tcgcggaagc
      241 gctccgggat cagcggcgcg gcatagcgat gatagaggcc gagcgattga tgcgggaaca
      301 tcgccgccag caggaagacg acgagggcgg cgaagaacgc tgccgtgccg acgagggcca
      361 cagactcgat gctccccgcg aagccggagt cgtgggtgag gctggccagg ccgcgcaggt
      421 tgacgaacac gaacatcagc atcaccacgc cgtcgaagat gcgctcgacg acgatcgtgg
      481 ccaggctcgc gctcacggcc accccctctt tgcggcgcag catgtaggcg cgcagcactt
      541 cgcccgcgcg ggcgggataa atgttgttgc ccatgtaacc gatggcgacg acggggaaca
      601 tggagggcgt ggagatggac ttgagaggcc gcaggaggta atgccagcgc cacgcgcgcg
      661 cccaaacggc cacaaagtag atggcgatgc ccggcaacag ccaccagtag tttgcggttt
      721 tcatcgccga ccagacgtcg gccagtttca aactcctcag cgcgagccac agaaaaaatg
      781 cgctgatgag aacccccacc cagaattgcc agcgtttcat ggtgtccgac gtgtctttcg
      841 atgacaagca aacggcgggg tctggccccg ccgcccgtta cgcacgcgat tataccatct
      901 tgcccacaac aaaaacgggc gaggcattgt gctgcgcctc gcccgctggc ctgctgtatt
      961 cggttgtcgg ttacgccggg gccgtctccg gcagcatctt ctcgagcacc atctcgagcg
     1021 cgcgggtgtg gcagcaccag cggtgcatca gaaagaactc acagtcgcaa tggaaaacgc
     1081 cgttgtcgaa gttgatcgag tggggattgt tatcgccact gaaggtgacg ctgaattgcg
     1141 tgaagcggaa ccgttcgcgt tcctcagcat atttcttggc tttcgcgatc ttcccggcat
     1201 agtccagcat gttttcctcc tatgtggaaa agatgtgaga aacaaaaagc acgcaagcgt
     1261 gaacacgctg cgtgctctct atgggatatg cggttctgcc agcagtaaca ctcggctcat
     1321 ttggccgttt atacctccac acccgggctg acaatctaca tgcagtatag gcggagtcgg
     1381 cgggagagtc aactgccgga ttccctacgt tccgggtgaa cctagtccta tgaagattaa
     1441 cgattggtga gaaattctgg ccctacgtct ggggttcgcg ccacagccgg aagttctcca
     1501 gaatggagcc gccgatgaat tcccgcagaa tcgaattgtc cggaatgtga tcagacggcg
     1561 cggggcgaaa ccactgcaag aacgaaagca gccggttggc ctccaaatat tccggcgtgt
     1621 ccgggcgcac ttgcaggagc agcggctcgg ccaacgggcg cagcaccgcc agctcgtgca
     1681 ccagcgccga gttgaaaacc gcgtcgctgt tttcctgaaa gggaaagatg tgcaccttct
     1741 cgccgtgctt cactgattcc cagcgccgca gcgtctcggc ggcgttgtag ccgcgcgtgg
     1801 ccgcgtcgcg cacgacgcgc cggatgagac gcgagtcggt ggtgctcacg cggttgtgcc
     1861 ggtcgaggtt caattgcgtg agcgcggaga cgtacacgcg atagacacgc tcgggcggca
     1921 ggcccggaac cagcgcgggg ttcaacccgt ggatgccctc gacgatcaca atgtgatccg
     1981 ggccgagcgt gaccatcggg ccgggttcgc tccggccggt gaggaagttg taatgcggca
     2041 gtcgcggtgc gcccccggcg atcaaatcgc gcagattttc gttgaataga gcgagatcga
     2101 gggcctggat ggtttcgtag tcaagctggc cctgctcgtc gcgcggcgtc tgctcgcgat
     2161 ccacaaagta gtcgtcgagc gcgagcggaa agggatggag accctgagcc agcaactgga
     2221 cggcgaggcg cttggagaaa gtcgtcttcc ccgaggccga cggcccggcg acgagcacga
     2281 tcttgatccg ctcgcgcccg gcggcgatgt cggcggcgat gcgggccagg cgggattcat
     2341 gaaaggcctc caccaccagc gagatttccg ggagacgccc ggcgacgatg gcgtcgttca
     2401 gcgcgcccac gctgcggatg cccagccggt cgagccagtg tcccgcttcc tcgaacacct
     2461 cgaacaattt gggatagggc gtgatgggcg aaagttcggt cgggttgctc tggtgcggga
     2521 actgcagcat gaagcccggc gggtaagcgt gcaacgcaaa ggtgcgcagg tagcccgccg
     2581 agggcaccat atacccctgg aagtaatcac ggtggccgcg cagcgagtac agcaccagcg
     2641 tgcccttctg gcgatgggcc agcaggcgga ctttgtcttc ctcgccccgc tggcggaaga
     2701 gttccatcgc ctgctgcacc gggacctgcg ttttgacgaa cggctcgtcc tgcgccacga
     2761 tcgcgcgcat gcgcgcctcg atcttctgca gctcggcctg ggagaacggt tcgcgcccgc
     2821 gcacctcgca gaagtaacct gccgccgtgg aggcggcgtg ctcgatgaac acttcggctt
     2881 ccggaaacac ttcggcagcc gccgtcacca gcaggaacgt cagcgaacgg cggtagatgc
     2941 gcgcgccgtc cgtgtcggag tgggagaccg ggatcacctc ggcgtcggcg gtcaggggcg
     3001 cagtcagctc gcgcagtttg cgattgatga ccgcggcgac cacggggggg tcggcgaggc
     3061 catcggctac ccttagaatg tcggccagcg gcgtgcccgg cggcgcttcg aagatgcggc
     3121 catcagggaa gcgtgcctgc acgcactcgc gcggcttgcc ggggaggatg ctcacgccaa
     3181 tctccaattc gggatcacgt ccatgtccag cgcgtcgcgc tggccggcca ggtagcggaa
     3241 gttcccggcg gcggcgatca tcgcggcgtt gtccgtgcac aggatcagcg gcgggacacg
     3301 caccgggacg ctcgcgcgcc gctcgaactc ggccttgagc gttttgttcg cgctcacgcc
//
LOCUS       DBNGWZ010000059         2170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2398014, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000059 DBNGWZ010000000
VERSION     DBNGWZ010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2398014"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="AC16CX_01710"
     CDS             complement(<1..874)
                     /locus_tag="AC16CX_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3011255.1"
                     /translation="MGGSSEPALMDDERRHRHLFEDNPLPMWIIDPESMRFLDVNQAA
                     IRTYGYSRDEFLSMTTFDLRLPEDIAAYRWYLDGRDASKTTVKRQTRHRRRDGGIIDV
                     EVTAHPLSFGGRLARLVLINDITERVRAERELHESEQRYRDLFDYNPSPMWVYDNDTL
                     AFVAVNEAATRQYGYTREEFSGMTLVDLFPPKDAAPFVEYIRRRDPRKVATGSYRHRR
                     KDGSLLDVEVLSSLLPHPQRVLRIVLATNVTERKRTEEALRESEERFRATFEQAAVGM
                     ALRGIDPRNPRWLRV"
     gene            complement(1045..1968)
                     /locus_tag="AC16CX_01715"
     CDS             complement(1045..1968)
                     /locus_tag="AC16CX_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3011256.1"
                     /translation="MTIAFITHGDCLLHDMGAHHPECPARLRAIEDQLTASGMINHLQ
                     HVEAPLAGRAQLERVHAPDYITALEREAPEHGLVHLDPDTAMNPHTLAAALRAAGAAV
                     LATDMVVRGEAAAAFCNVRPPGHHAERARAMGFCLFNNVATAAAHALHAHGLKRVAIV
                     DFDVHHGNGTEDIFRDDPRVMMVSTFQHPFYPYSGIDGRSERMVNIPLAAYSGGKEFR
                     AAVEQHWLPALEAFKPEMLFVSAGFDAHRDDDMAMLNLVEADYGWVTERIKAVAERHA
                     AGRIVSLLEGGYELHALGRSVAAHIKKLGDI"
BASE COUNT          358 a          733 c          680 g          399 t
ORIGIN      
        1 tcacgcgcag ccagcgtggg ttgcgcggat cgatgccgcg cagcgccatg cccaccgccg
       61 cctgctcgaa agtggcgcgg aagcgctctt cgctttcgcg cagcgcttcc tccgtgcgct
      121 tgcgctccgt gacgttcgtt gccagaacga tccgcagcac ccgttgcgga tggggcagca
      181 ggctcgataa aacctccacg tcgagcaggc ttccatcctt gcggcggtga cggtacgatc
      241 cggtcgccac cttcctcggg tcacgccgcc ggatgtattc cacgaaggga gcggcgtctt
      301 tcggcggaaa cagatccacg agggtcatcc ccgagaattc ctccctggtg tagccgtact
      361 gccgtgtcgc cgcctcgttg acggcgacga aggcgagcgt gtcattgtcg taaacccaca
      421 tcgggctggg gttgtagtcg aacaggtcgc gatagcgctg ctcgctctcg tgcagttcac
      481 gctcggcgcg cacccgttcc gtgatgtcgt tgatcagcac cagccgcgcg aggcggcccc
      541 cgaagctcag gggatgagcc gtcacctcca cgtcgatgat gccgccgtca cgccgccgat
      601 gccgcgtttg ccgcttgacc gtcgtcttcg acgcatcgcg gccgtccagg taccagcggt
      661 acgccgcgat gtcttccggc aggcgcagat cgaacgtcgt catcgaaagg aattcgtcgc
      721 gcgaatagcc gtaggtgcgg atcgccgcct ggttcacgtc caggaaccgc atgctctccg
      781 gatcgataat ccacatcggc agcgggttgt cctcgaacaa atggcggtgg cgccgctcat
      841 catccatgag cgccggctcg ctgcttccgc ccacgggcgc gggagtttga ccgccttcga
      901 gggcgccgcg gagcgctctc gcgggcgctt ttctgcgtcg tctaaccagg ttcactcctc
      961 ctgccttgcc atcctgatgg ccaatctacc ccaccgcggg acatcatgga caaaaacagc
     1021 gtggtggctg ctgccaaccg cggttcagat atcgcccagt tttttgatat gcgccgccac
     1081 actgcggccc agcgcatgca gctcatagcc gccttcgaga agcgacacga tgcgtcccgc
     1141 cgcatggcgc tccgcgaccg ccttgattct ctccgtcacc catccgtagt cggcttccac
     1201 caggttgagc atggccatgt cgtcgtcgcg gtgggcatcg aagccggccg agacgaacag
     1261 catctccggc ttgaacgcct cgagcgccgg caaccagtgc tgctcgaccg cggcgcggaa
     1321 ttccttccct ccgctgtaag cggcaagcgg gatgttgacc atccgctcgg agcggccgtc
     1381 gatgccgctg taggggtaga acggatgctg gaaggtggac accatcatca cgcgcggatc
     1441 gtcgcgaaat atgtcctcgg taccattgcc atggtggacg tcgaaatcca cgattgcaac
     1501 ccgtttcagc ccatgtgcgt gaagcgcatg ggccgcggcg gtggcaacgt tgttgaacag
     1561 gcagaagccc atggcgcggg cgcgctcggc gtggtgcccc ggtggacgca cgttgcagaa
     1621 cgcggcggcc gcttcgccac ggacgaccat gtcggtcgcg agcaccgcgg cgcctgcagc
     1681 acgcagcgcc gcggcaagcg tgtgcgggtt catcgcggtg tcgggatcga gatggaccag
     1741 accgtgctcc ggcgcctccc gctccagcgc ggtgatgtaa tccggcgcat gcacgcgctc
     1801 gagctgggcg cggccggcga gcggcgcctc gacgtgctgc agatgattga tcatcccgga
     1861 ggcggtcagc tgatcctcga tcgcgcgcag ccgcgccggg cactcgggat ggtgcgcgcc
     1921 catgtcgtgc aacaggcagt cgccatgggt gatgaaggcg atcgtcactc cctaattata
     1981 atattcgcct ccacatccgc tacgccgcgc cacacgtatc ttgcgctttc catgcagtcc
     2041 tatccctccc accttgtcca acggctgacg ctgaaggacg gcaccgcggt cacgctgcgc
     2101 ccgatccgcc cggaggacgc ccggatcgaa caggagttcg tgcgcaacct ctcggatgaa
     2161 tcgcgttatt
//
LOCUS       DBNGWZ010000060         8374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2523979, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000060 DBNGWZ010000000
VERSION     DBNGWZ010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2523979"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC16CX_01720"
     CDS             <1..951
                     /locus_tag="AC16CX_01720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3011257.1"
                     /translation="LGCMGMSISYGEPNDEESIATIHRAIELGVNLLVTSDAYGNGVN
                     EELIARAIKGRRNKVLLATKFGNLALASRSAEGGAPALTGGHPDYVPQACDRSLKRLG
                     VDVIDIYGLHRVDKTVPIEDTVGAMKRLVEQGKVRYLQLSEAGAQTIRRAHKVHPITA
                     VETEYSLWSRDVEKGILPTCRELGIGYMAYAPLGRGFLTATIKTLDALLPKDRRREHP
                     RFSADNIGKNAQLLAPLEDIAAAKKCTPSQVALAWLLAQGEDIVPIPGTKRRSYLEQN
                     CMAINVKLTKEDIATLSKAFPLNITAGTRYPEKQLAGLGI"
     gene            1015..1575
                     /locus_tag="AC16CX_01725"
     CDS             1015..1575
                     /locus_tag="AC16CX_01725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3011258.1"
                     /translation="MKLNLGCGDKILAGYVNVDVAESRAGMRPDVLCDLHALTPFEDN
                     SADEILSVHVVEHFWRWEVVGVLREWVRVLKPAGLMILECPNLRSACETFLANPEVHA
                     GPGPEGQRTMWVFYGDPSWRDPLMVHRWGYTPQSLASVMAEAGLVNIRQEPAQFKLRE
                     PRDMRLVGEKPSHEPRGYQSVWKISL"
     gene            1597..2652
                     /locus_tag="AC16CX_01730"
     CDS             1597..2652
                     /locus_tag="AC16CX_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3011259.1"
                     /translation="MAKALWLIPPFVMPYASGENPEQSLLACPRASARLRIAVAADEW
                     RRRGNENVFWDPGVTDAGERFDWKGAEICVVPKFYHDIPLQPWLDACSAVRSSGCRLV
                     IDVCDYPFEKQFPVPAFYSEVLKICDAVVVNSERMAEMIAPHARQRPVVIEDAILDAM
                     SSPDFAPGERLEVLWFGHPVNLHYLDARLEGLARFAQRKRCRLTIVTEYSREVGEWTE
                     SIPPRFAFAFETRFVPWSLEAMPTALRNCDLVLIPSDPADAFKAGASANRIAEALNAG
                     RFPVASPLRSYLQFADAAWLGQDLFAGVHWALANREDVLARIRRGQELVKEKFAADRI
                     GRQWHELFESLLSAPHL"
     gene            2703..3347
                     /locus_tag="AC16CX_01735"
     CDS             2703..3347
                     /locus_tag="AC16CX_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HIR3011260.1"
                     /translation="MKDAGLAYLKWYYETNVWKNLRYRGVRTLKLPLDMWNYQEIIFE
                     NSLHWVVETGTRHGGSAMYFADLLAAGVREGCVVSVDVTHEDVHPLARAHPKIRLLLG
                     DSAATETVGMIRRVIPDNRSHAMLLILDSDHAAKHVLRELNILVPLLRPGDYLIVEDT
                     IVNGHPVRPDFGPGPLEAVGAYLGQNPDKLVHDSERESKFGCTFAYRGHYRIRA"
     gene            3745..6063
                     /locus_tag="AC16CX_01740"
     CDS             3745..6063
                     /locus_tag="AC16CX_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3011261.1"
                     /translation="MPEPPIHEQQAPSIASAIQEAVCFHQQGRLKEAEQLYQAVLGAQ
                     PGHFEALHFLGILRAQQGDWEAAARLISLALERNPRSADAHSHLGNLFYHLGRHDEAL
                     ASYDKALAIGPDFPEAFYNRGNALQALHRYDEAIASYDRALALRPAYAEAHNNRANVL
                     QELRRYEEAVASYDRCLAINPYDVEALNNRGSALHNLKRHDEAIASCDRALAIKADHV
                     HALNNRGNILQDLGRYNEALACYDRALTLKPDYPEALSNRGNVLQDLGRYNEALACYD
                     RALALQPDYPEALNNHGDALLKLKRPDEAARSFHRLLEAVPDYSFAKGELLYAKMASC
                     DWEGLTELAQCIENDIQAGKKSAEPFGCQAILCSAQSLRRCAEIYAGEKFPSSPIKLW
                     NGEKYRNARIRIGYVSGEFCNQATSILMTGLFERHDRNRFELFAFDNGLDDASEIRGR
                     IRRAFDEMVDITRLGDREAAAAIKQRKIDILVNLNGYFGRARQGIFSLRPCPIQVNYL
                     GFPGTLGADCMDYILADRHVIPPEDQACYTEKVVYLPECYQPNDSKRAIAERTPTRAE
                     ARLPDSGFVFCCFNNNYKITPEVFDSWMRLLKEVPGSILWLLEDNAAASRNLRREAEA
                     RGVAPGRLVFASRMNLDEHLARHRLADLFLDTLPYNAHTTGSDALWAGLPLLTCQGTT
                     FSGRVAASLLHAVGLLELITRSLDEYEARALELAASPAMLADIRVKLARNRTTYPLFD
                     TDRFRHHIESAYITMWERTQRGEPPTSFAVQP"
     gene            complement(6157..6456)
                     /locus_tag="AC16CX_01745"
     CDS             complement(6157..6456)
                     /locus_tag="AC16CX_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HIR3011262.1"
                     /translation="MSKSKKKSRAAPAIAGEPFFADQAQEDLAWWRRANPRMLARINV
                     LIEDIRLHPFTGIGKTEPLKHDWQGYWSRRITDEHRIVYRVEAGRIYILQLRYHY"
     gene            complement(6407..6661)
                     /locus_tag="AC16CX_01750"
     CDS             complement(6407..6661)
                     /locus_tag="AC16CX_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3011263.1"
                     /translation="MLDTISYTQLRNRLAKTLDKVTQDHAPVLITRQKGKAAVLISVE
                     DYSALEETAFLLRSPKNAARLRVAREQVEKEIARRARHRR"
     gene            complement(6893..>8374)
                     /locus_tag="AC16CX_01755"
     CDS             complement(6893..>8374)
                     /locus_tag="AC16CX_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3011264.1"
                     /translation="LAWFNLGNVVQEQGRQQEAEEYYRRAIELDPAFADAHTNLGGVL
                     RALGRFDEAEQAYRRCLELQPDSATAYCNLAAVLIDRGRFAEAESLCRQALLRSPRSE
                     ELYLLLGSACTHQGRLTSALEAFRAARVAAPHNPRALWAYGYALLRTGEQTEGRQWLE
                     RAVALQPDLPEFQGALSGIYLSLGDLQAGWMEYQRRPAKRSFLAENPHLQLAVDLPRS
                     LSGQKVCLLREQGLGDELFFLRFAAAVKSRGADVTYCAHHKIASMLARVPVLDRVIGQ
                     TDPLPAADAVLLVGDLPHALGAWDFPPPLALAPLPERLESIKQRLAALGPPPYLGLTW
                     RAGTAPEQQRGMDWVLHKMIPLESLGASLRGLNGTLVALQRNPEASEIGKLARVACKP
                     IHDLTALNEDLEAMLAVLALLDDYIGVSNTNMHLRAGSGRTAKVLVPQPPEWRWMATG
                     DESPWFPGFRVYRQQPSGSWDDAFERLRANLFAQFGRSARTAP"
BASE COUNT         1640 a         2557 c         2617 g         1560 t
ORIGIN      
        1 ctgggctgca tgggcatgtc catcagctac ggcgagccca acgacgagga atccatcgcc
       61 accatccacc gggcgatcga gctcggcgtc aacctgctcg tcacgtccga cgcctatggc
      121 aacggcgtca acgaggagct gattgccagg gcgatcaagg gccggcgcaa caaggtcctc
      181 ctcgccacca aattcggcaa cctcgcgctg gcgagccgga gcgcggaggg tggcgcgccg
      241 gcgctcaccg gcgggcatcc cgattacgtg ccgcaggcgt gcgacaggag cctgaagcgc
      301 ctgggcgtgg acgtaatcga catctacggc ctgcaccgcg tggacaagac cgtgccgatc
      361 gaggacacgg tcggtgccat gaagcggctg gtcgagcagg gcaaggtgcg ctatctccag
      421 ctgtccgagg ccggcgcgca gaccatccgg cgcgcgcaca aggtccatcc gatcaccgcg
      481 gtcgagactg agtactcgct gtggagccgg gacgtggaaa aggggatcct gccgacctgc
      541 cgcgagctcg gcatcggcta catggcgtat gcgccgctcg ggcgcggctt tctcaccgcc
      601 accatcaaga cgctcgatgc cttgctgccc aaggaccggc ggcgggagca cccgcgcttc
      661 agcgcggaca acatcgggaa gaacgcccag ctgctcgcgc cgctcgagga catcgcggcg
      721 gcgaagaaat gtacaccgtc acaggtcgcg ctcgcctggc tgctcgcgca gggcgaggac
      781 atcgtgccga ttcccggcac caagcgccgc agctatctcg agcagaactg catggcgatc
      841 aatgtcaagc tgacaaagga agacatcgcg acgctgtcta aagcgtttcc gctcaacatc
      901 accgccggca cccgctatcc cgagaagcag ttggcggggc tcggaatatg atcgggcggc
      961 acgatgtacg gccaagtagc ttgcggataa ggccttcgcc agcagatggt ctccgtgaaa
     1021 ctgaatttgg ggtgtggcga caagatactt gccggctacg tcaacgtcga cgtggcggaa
     1081 agccgggcgg ggatgcggcc ggacgtgctg tgcgacctgc acgcccttac tccatttgaa
     1141 gacaactccg ccgacgaaat tctgtcggta catgtcgtgg agcacttctg gcgttgggaa
     1201 gtcgtgggcg tcctgcggga atgggtccgc gttctgaaac ccgccgggct gatgatcctg
     1261 gagtgcccga atctgagatc cgcgtgcgaa acgtttcttg ccaatccgga ggttcatgcc
     1321 gggccgggac cggaggggca gaggaccatg tgggtctttt atggcgatcc atcctggcgc
     1381 gatcccctca tggttcaccg ctggggatac actccgcaaa gtctcgcctc agtaatggcc
     1441 gaggccggcc tcgtaaatat ccggcaggaa ccggcacagt tcaagctgcg cgagcccagg
     1501 gacatgcggc ttgtcggcga gaaaccaagt catgaaccgc ggggctacca gagtgtctgg
     1561 aaaatatcgt tataagaaat tctggcttgg cccgacatgg cgaaagcgct ctggctcatt
     1621 cctcccttcg tgatgccgta tgcgtcgggt gaaaatcccg aacagtccct ccttgcgtgt
     1681 ccgcgggcga gcgcgaggtt gcgtatcgca gtcgccgcgg acgaatggag gcggcgcggt
     1741 aacgaaaacg tcttttggga ccccggcgta accgatgctg gggaacgctt cgattggaag
     1801 ggcgctgaaa tctgcgtcgt ccccaagttc taccacgata ttccgttaca gccctggctc
     1861 gatgcgtgtt cagcagtccg cagcagcggt tgccggctgg tgattgatgt ctgcgattac
     1921 cccttcgaga agcagtttcc cgtcccggcg ttttattccg aggtgctgaa aatctgcgat
     1981 gccgtggtcg tgaactcgga acgcatggcc gaaatgatcg cgccccacgc tcgccagcgg
     2041 ccggtggtta tcgaagacgc gatactggat gcgatgagca gccccgattt tgcacccggt
     2101 gagagactcg aggtgttatg gttcgggcat cccgtcaatc ttcactacct cgatgcgcgc
     2161 ctcgagggcc ttgcccgatt tgcgcaacgc aaacgttgcc gattgaccat cgtgactgag
     2221 tatagtcgtg aggtggggga gtggacggag agcattccgc cccgttttgc cttcgcgttc
     2281 gaaacgcggt ttgtgccatg gtcgctggag gccatgccga ctgctctgcg caactgcgac
     2341 ctcgtgctga ttccgagcga tcccgcggac gcattcaagg caggcgcttc ggccaaccgg
     2401 atcgccgagg cgttgaatgc cggccgtttt ccggtggctt ccccgctgcg gagctacctg
     2461 cagttcgcgg acgcggcgtg gctggggcag gacctcttcg ctggcgtgca ttgggcactc
     2521 gcgaatcggg aagatgtgct cgcgcgcatc cggcgcgggc aggaattggt caaggagaag
     2581 ttcgcggcag acaggatcgg gcggcaatgg cacgagttgt ttgaaagcct cttgagcgct
     2641 ccccacctct gatcgtctgc ctgttctcgt cgcatggctc ccgcagcaaa ggaggcaatc
     2701 gagtgaaaga tgccgggttg gcatacctga agtggtacta cgagaccaat gtgtggaaaa
     2761 accttcgtta tcgaggggta cgaaccctca agctgccgct tgacatgtgg aactaccagg
     2821 aaatcatttt cgagaacagt ctccactggg tggtcgaaac cggcacgcgg catggaggct
     2881 ccgcgatgta ctttgcggat ctgctcgccg ccggcgtgcg ggaaggttgc gtagtgagcg
     2941 tggacgtgac gcatgaggac gttcatccac ttgcgcgtgc gcacccgaaa atacgattgc
     3001 tcctgggcga cagtgccgcg acggaaacag tcggcatgat ccggcgggtg attcccgaca
     3061 atcgatccca cgcgatgctg ctgatcctgg attcagatca cgctgcgaag catgtgttgc
     3121 gcgaactcaa tatcctggtc ccgctgttgc ggccgggcga ttacctgatc gttgaagaca
     3181 cgatcgtcaa cgggcatccc gttcgtcccg attttggacc tggtcctctg gaagcggtcg
     3241 gcgcatacct gggtcagaac ccggacaagc tcgtccacga ctccgagcgc gaatcgaaat
     3301 ttgggtgcac ctttgcatat cgcggccact ataggattcg cgcctaactg acgaatgacc
     3361 agatcaggtc gtcatgcgat ccaggcatgc attgtaagac cggcacgatg aggcccggaa
     3421 atcttcctat gctgagcgaa agtcttgcgc gagatgctcc agcgcctgcg tccagtcgcc
     3481 gttcgcgctt tggcgataga tgcggaatcc cggtaaccag ggcgactcat cgcccgtggc
     3541 catccagcgc cactggcggg agcggcgcac gcttccctgc cgtgatattg acgcaatacg
     3601 cacgtggcca ccaatcccaa tccataccgc acggcccgcc acgcggaaaa aagtattacg
     3661 atataccgcg cgaataatcg aagccaaatt acctagtcag tcgggatgct tgttttcaca
     3721 cagacctgag ggaaaagcga cctgatgccg gagccgccga ttcacgagca gcaagccccc
     3781 agcatcgcaa gcgccattca ggaagctgtt tgctttcatc agcagggcag gctgaaagaa
     3841 gcggaacagc tctatcaggc ggttcttggt gcgcaaccgg gtcattttga agcgcttcat
     3901 ttcctgggaa ttctacgcgc tcagcaaggt gactgggagg cggcggccag actcatctct
     3961 ctcgcgctgg agcggaaccc gcgctccgca gacgcgcact cccatctcgg caacctgttc
     4021 taccacctgg gccgtcatga cgaggcgctt gcaagctatg acaaagcgct tgcgatcggg
     4081 cctgactttc ccgaggcgtt ctacaatcgg ggcaatgcgc tgcaagcgtt acatcgatac
     4141 gacgaggcga tcgcaagcta cgaccgcgcc cttgctctca ggcctgctta tgccgaggcg
     4201 cacaacaatc gtgccaatgt cctccaggag ttgcgacgct atgaggaagc cgtcgcgagc
     4261 tacgacaggt gtctggcgat caatccttac gatgtcgagg ccctcaacaa ccgcggcagc
     4321 gccctccaca acctgaagcg ccacgacgag gcgatcgcga gctgcgacag ggccctcgcg
     4381 atcaaagccg accacgtcca cgcgctcaac aaccggggca acatcctcca ggatctgggc
     4441 cgctataacg aggcgctcgc atgttacgat cgcgccctga cgctcaagcc tgattatccc
     4501 gaggccctca gcaaccgcgg caacgtcctg caggatctgg gccgctataa cgaggcgctc
     4561 gcatgttatg accgcgcctt ggcgttgcag cctgactatc ccgaggcgct caacaaccac
     4621 ggtgatgccc ttctgaagct gaagcggcca gatgaggccg cccgctcctt tcacaggttg
     4681 ctggaagcgg ttcccgacta cagtttcgcg aagggcgagc tgctgtacgc gaaaatggca
     4741 tcctgcgatt gggaggggtt gaccgagctc gcgcagtgca tcgagaacga catccaggcc
     4801 gggaaaaaat ccgcggagcc gttcgggtgc caggcgatcc tatgctcggc gcagtcgctg
     4861 aggcgttgcg ccgagatcta cgctggggaa aaatttccat cgtcgcccat caagctgtgg
     4921 aacggagaaa aataccggaa cgcgaggata cgtatcggct atgtgtcggg cgagttctgc
     4981 aatcaggcga cctctatcct gatgacgggg ctttttgagc ggcacgacag aaatcgcttc
     5041 gagctgtttg cttttgacaa cgggctggat gacgcgagtg agattcgggg aaggatcagg
     5101 cgtgcattcg atgagatggt ggacatcacg cgcctggggg atcgcgaagc ggcagcggcg
     5161 ataaaacaga gaaaaatcga catattggtc aatctgaacg gctatttcgg gcgcgcaaga
     5221 cagggaatct tcagcctcag accgtgcccg attcaggtca actatctggg gtttccgggc
     5281 accctcggcg cggactgcat ggactatatc ctcgcggacc gccatgtcat accaccggag
     5341 gatcaggcct gctacacgga aaaagtcgta tatctcccgg agtgctatca acccaatgac
     5401 tcgaagcggg caatcgcgga gcgcacgccc acccgtgccg aggccaggct gcccgatagc
     5461 ggtttcgtct tctgctgctt caacaacaac tacaagatca cgccggaggt gtttgacagt
     5521 tggatgcggc tgttgaaaga ggtgccaggc agcatcctgt ggctgctgga ggacaacgcg
     5581 gctgcatcgc gcaatctaag gcgcgaagcc gaggcgcgag gcgttgcgcc cgggcgattg
     5641 gtatttgcgt ctcgcatgaa cctggatgag cacctcgcgc gccacaggct ggcggacctg
     5701 ttcctggaca cgctgcccta caacgcccac acgaccggca gcgatgcgtt gtgggccggg
     5761 ttgcccctgc tgacgtgtca aggcaccacg ttctccggac gggtggcggc gagcctgctg
     5821 cacgcggtgg gcctgctcga gctgattacc cgcagcctgg acgaatatga agcgcgcgcg
     5881 ctcgaactgg ccgccagccc cgccatgttg gctgacatac gcgtgaagct ggcacgcaac
     5941 cgcacgacct atccgttgtt cgatactgac cgcttccgcc accacatcga gtcggcttac
     6001 atcacgatgt gggagcgcac tcagcgcggg gagcctccca caagcttcgc cgtgcagccc
     6061 tgaatggacc gaaccacgat tcacgaatca cgactcacgc cttttcaggg tgccgcggtg
     6121 aaacgctctt tccgtcacgg gtttgctctg ctggagctaa tagtggtaac ggagctgcag
     6181 aatgtagatg cgtcctgcct cgacacgata gacaatgcgg tgctcatcgg tgatgcggcg
     6241 ggaccagtag ccttgccagt cgtgcttgag cggttcagtc ttgccgatcc cggtaaaggg
     6301 atgcaggcgg atatcctcta tcaggacatt gatccgtgcc agcatccgcg ggttggccct
     6361 gcgccaccag gcgaggtctt cctgcgcctg gtcggcgaag aacggctcac cggcgatggc
     6421 gggcgcggcg cgcgatttct ttttcgactt gctcacgagc cacgcgcaac cgggcggcgt
     6481 tcttcgggct gcgcagcagg aaggcggtct cttcaagcgc cgagtaatcc tccaccgaaa
     6541 tcagcacggc ggctttgcct ttctggcggg taatgagaac gggggcgtgg tcctgcgtta
     6601 ccttgtcaag cgtcttcgcg agccggttgc gtaactgtgt gtaagagatg gtatcgagca
     6661 tgtccatcct ctccctgttc aatgttctgt acaggccaaa ttctaacacc aaaggaccac
     6721 gccatgatgt gatcgtgcgt caaacccggc aggcagcgaa ccgggggccc tgcagcttgt
     6781 gccgttccaa catgttcgga caagtgcagg gttgcggatg cactacacct acctgcattc
     6841 cgcggccatc gcgtattcgg gacaacgagt tggaacggca ctagtttcgt gctcaagggg
     6901 cagtgcgggc gctccggccg aactgggcga agaggttagc gcgcagcctc tcgaacgcat
     6961 cgtcccaact gccgctgggt tgttgccggt aaactctgaa cccgggaaac cacggcgatt
     7021 cgtcgccggt ggccatccag cgccactccg gcggctgggg caccagcacc ttcgcggtcc
     7081 ggcccgaccc ggcgcgaaga tgcatgttgg tgttgctcac gccgatgtag tcatcgagca
     7141 gcgccagaac agcaagcatg gcctcgagat cctcgttgag cgcggtgagg tcgtgtatcg
     7201 gcttgcaggc gactcttgcg agtttgccga tctcgctggc ttccggatta cgctgcagcg
     7261 cgacgagcgt gccgttcagg ccccgaagcg aggcgcccag actttcaagc gggatcattt
     7321 tgtgcagcac ccaatccatt ccgcgctgct gctcgggagc ggtcccggca cgccaggtca
     7381 agccgaggta cgggggcggc cccagcgccg ccagccgttg tttgatggat tcgagccgtt
     7441 ccgggagcgg tgcgagcgcg agcggcggcg ggaaatccca tgcgccgagc gcatgcggca
     7501 aatcacccac cagcaacaca gcatcggcgg ccggcagcgg atcagtctgg ccgatcaccc
     7561 gatcgagcac cggcacgcgc gcgagcattg atgcgatctt gtgatgggcg cagtacgtga
     7621 cgtccgcccc gcgcgacttc accgccgcgg cgaaccgcag gaagaacagc tcgtccccca
     7681 aaccctgttc gcgcagcagg cagactttct gtcctgacag gctacggggc aggtccacgg
     7741 ccagctgcag gtgcgggttt tccgcaagga agctccgttt tgcaggccgc cgctggtact
     7801 ccatccagcc ggcttgcaga tcacccagcg acaggtaaat cccggagagg gcgccttgaa
     7861 attccggtaa atccggctgc agcgccaccg ccctttccaa ccactggcgc ccttccgtct
     7921 gctcgccggt tcggaggagc gcgtagccgt atgcccatag cgcgcgcggg ttgtgtggtg
     7981 cggcgaccct cgcggcgcgg aacgcctcca gcgccgaagt aagccgtccc tggtgcgtgc
     8041 aagcgctgcc cagcaataaa tacaactcct cggatcgggg cgagcgaagg agcgcttgcc
     8101 gacagaggct ctcagcttcg gcgaaccggc cgcggtcgat caacaccgcg gcgaggttgc
     8161 agtaagccgt cgccgaatcc ggttgcaact cgagacatct ccggtacgcc tgttccgcct
     8221 cgtcgaagcg cccgagtgcg cgcaataccc cgccgagatt ggtgtgtgca tcggcaaacg
     8281 ccgggtcgag ctcgatggcg cggcggtagt actcctccgc ttcctgctgc cgcccctgtt
     8341 cctgcactac gttaccgagg ttgaaccagg cgag
//
LOCUS       DBNGWZ010000061         3682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2398131, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000061 DBNGWZ010000000
VERSION     DBNGWZ010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2398131"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16CX_01760"
     CDS             complement(<1..143)
                     /locus_tag="AC16CX_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3011265.1"
                     /translation="MATDFRQEFIRFTISQNVLRFGEFKTKAGRLSPYFFDAGLFYDG
                     AAL"
     gene            242..1024
                     /locus_tag="AC16CX_01765"
     CDS             242..1024
                     /locus_tag="AC16CX_01765"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR3011266.1"
                     /translation="MKIITLNLNGIRSAARKGFFPWMTRTRADVVCVQELKAQAPDLD
                     EDMRAPGSFRGYFHCAEKKGYSGVGVYSRREPDRVVEGIGRRDIDAEGRYLRADFGPL
                     SVISVYLPSGSAGEHRQTIKFKFMDHFLPHLKDLAACGREILLCGDWNIAHKEIDLKN
                     WRSNQKNSGFLPEERAWLTRVFDELGWVDVYRRLRPDTTGEAYTWWSNRGQAWAKNVG
                     WRIDYHIATPKLAAAAKRVAIYKKERFSDHAPLTIDYDYPLA"
     gene            1005..2324
                     /locus_tag="AC16CX_01770"
     CDS             1005..2324
                     /locus_tag="AC16CX_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR3011267.1"
                     /translation="MTTPWLSGLRSKLLEGVSPYFESAPLAALFLGISSGFTFAMIGA
                     TLTTRLAQHGVTKSAVTAFALTFLAYNIKFLWAPIVDNVRLPVIGRFGQRRSWLWLVG
                     VLVMAAVAFLGIADPGDSLYVVAIAAIVMGVAGATFDIIIDAYRIELLEPRQLGVGSG
                     MSQYGWRIGAAAAGALALVLAARVGWTAAYLACAVFALPAMLIGMLMGEPVRHREPAK
                     PRGVVEAVIAYFSPLTEFLERQGALVVLVFVLIHKIGDTLANLTLRLLFQDLGFTNDE
                     VAFYDVGVGFVALLIGIFLGGILYARMGMKRAVLISLILMAVSNLSFAALATAGHTNV
                     GMAAAVGFENFASGIGGVTVVAYLSALCNLRFTATQYALLSALASIAGRFLTGTTVGA
                     LIDAIGYVNFYLLTTLVALPGVILFWFMIRSGLADLSIGSAGKEGAG"
     gene            2371..3255
                     /locus_tag="AC16CX_01775"
     CDS             2371..3255
                     /locus_tag="AC16CX_01775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3011268.1"
                     /translation="MTIPLSVHTGGTEIPVPEDFRWPGGKRIAVIFRMAFEAWSDDHW
                     PGISPMGNPLKPGFPDLNARGWAEYGYRRGVFRVLDALARHRVRATVMVCGILAERHP
                     RIVRQIADAGHEVAAHSYTMDMMSIYMNEEEERGNIRRNVELIERATGKRPVGWINPR
                     GTPSPNTARLLAQEGFEWHGDTLNDDLPYLVRFGSKSIVALPSTMEVNDLPLTMKHGQ
                     PPSVYVENFEAWLEYARKREKGACKIDPTVHAHVFGRPAGTWAFERVMEIARKSKDIW
                     IGTRAEVAAHVRKVLKNP"
     gene            3338..>3682
                     /locus_tag="AC16CX_01780"
     CDS             3338..>3682
                     /locus_tag="AC16CX_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3011269.1"
                     /translation="MRLVTFVPRKGAATRVGVVRDGKQVIDLVEAGGKPPFDPADMLS
                     LIAAGAKALKWTDGVAARAKKTLPLDKVKLAAPIPRPRKNVFCVGWNYLDHFEEGAKS
                     RTQKVDLPEHPAF"
BASE COUNT          645 a         1141 c         1210 g          686 t
ORIGIN      
        1 ttcagcgccg cgccatcgta gaacagaccg gcatcgaaga agtacggcga gaggcgcccc
       61 gccttggtct tgaattcgcc gaagcgcagg acgttctggc tgatggtgaa gcggatgaac
      121 tcttgcctga aatcggtcgc catattggac tgaaatttac ccctgacacg ggcacgttga
      181 ccggcgcgcg ggtggcgcgt ccggtcgaac gggcatcgac ccagccgctc cggacttaac
      241 gatgaaaatt ataacgctga atctcaacgg catccgttcc gcggcgcgca agggcttctt
      301 cccgtggatg acgcgcaccc gggcggacgt ggtgtgcgtg caggagttga aggcacaggc
      361 gcccgacctg gacgaggaca tgcgcgcgcc gggaagtttc cgcggctatt tccactgcgc
      421 ggagaagaag ggctacagcg gggtcggtgt ctacagccgg cgcgagcccg accgcgtggt
      481 ggaaggcatc ggccgtcgcg acatcgatgc cgagggacgc tacctgcggg ccgatttcgg
      541 tccgctgtcc gtgatctcgg tctatctgcc ttcgggttcg gccggcgagc accggcagac
      601 gatcaaattc aagttcatgg atcatttcct tccccatctc aaggatctcg cggcgtgcgg
      661 ccgcgagatc cttttgtgcg gtgactggaa catcgcgcac aaggaaattg acctcaagaa
      721 ctggcggagc aaccagaaga actcgggttt cctgcccgag gagcgcgcgt ggctgaccag
      781 ggtgttcgac gaattgggat gggtggatgt ttaccgccgg ctgcgtccgg acacgaccgg
      841 cgaagcctat acgtggtgga gcaaccgcgg gcaggcgtgg gccaagaacg tgggatggcg
      901 catcgattac cacatcgcca cgccgaaact cgcggccgcc gcgaagcggg tcgcgatata
      961 caagaaagag cgattttccg atcacgcgcc gttgacgatc gactatgact accccttggc
     1021 ttagcggcct gcgcagcaag ctgctggagg gtgtcagccc gtatttcgag tccgcgccgc
     1081 tcgccgccct gttcctgggc atctcctccg gcttcacctt cgcgatgatc ggcgcgactc
     1141 tcaccacgcg tcttgcccag cacggagtca ccaagagcgc agtgaccgct tttgcgctca
     1201 ccttcctcgc ttacaacatc aagttcctct gggcgccgat cgtcgacaac gtccggctgc
     1261 cggtgatcgg caggttcggg cagcggcgca gctggctatg gctggtcggt gtgctggtca
     1321 tggctgccgt cgcttttctc ggtatcgccg acccgggcga ctccctctat gtcgtcgcga
     1381 tcgccgcgat cgtcatgggc gtggccggcg ccacgttcga catcatcatc gatgcgtatc
     1441 gtatcgagct gctcgagccg cgccagctcg gcgtaggctc tggcatgtcg caatatggct
     1501 ggcggatcgg cgcagcggca gccggcgccc tggctttggt cctcgccgcc cgggtgggct
     1561 ggaccgccgc ctatctggcc tgcgccgtct tcgcgcttcc ggcaatgctg atcggtatgc
     1621 tcatggggga gccggtccgg caccgcgagc ccgccaagcc gcgcggggtc gtcgaggccg
     1681 tcatcgcata tttcagcccg ctgaccgagt tcctcgagcg tcagggagcg ctcgtcgtgc
     1741 tggtgttcgt gctcattcac aagattggcg acaccctcgc caacctcacg ctgcgcctgc
     1801 tgttccagga tctcggcttc accaacgacg aggtggcctt ctacgacgtc ggggtcggct
     1861 tcgttgccct gctgatcggc atcttcctcg gcggaatcct ctacgcgcgg atgggaatga
     1921 agcgcgccgt cctgatcagc ctgatcctga tggcggtgtc gaatctgagc ttcgccgcgc
     1981 tggcgacggc cggccatacc aatgtcggca tggccgcggc ggtcggtttc gagaatttcg
     2041 ccagcggcat cggcggcgtg acggtggtcg cctacctatc cgcgctgtgc aacctgcgct
     2101 tcactgcgac ccagtatgcg cttctctccg ctttggcgag catcgctggc cggttcctca
     2161 ccggaacgac cgtaggagcg ctgatcgatg cgatcggtta tgtgaatttc tacctgctga
     2221 ccacgctggt cgcgctgccc ggcgtgatcc tcttctggtt catgatccgc tccggacttg
     2281 ccgacctgtc gatcggctcg gcaggcaagg agggcgctgg atgacgcgcc ttgcggacgg
     2341 cgcgccggcc aaactcgaca aggagaacgc atgactattc cactctctgt gcacaccgga
     2401 ggcaccgaga ttccggtgcc ggaggatttc cgctggccgg gcggcaagcg catcgcagtc
     2461 atcttccgga tggcgttcga agcgtggtcg gacgaccact ggccggggat cagcccgatg
     2521 ggcaatccgc tcaagccggg gtttcccgat ctgaatgcgc gcggctgggc ggaatacggc
     2581 taccggcgcg gagtgttccg cgtcctcgac gcgcttgccc gccaccgcgt cagggcgacg
     2641 gtgatggtct gcggcattct cgcggagcgc catccgcgga tcgtcaggca aatcgccgat
     2701 gcaggccatg aggtcgcagc tcattcttac accatggaca tgatgtccat ctacatgaat
     2761 gaagaggagg agcgcggtaa catccgccgc aacgttgagc tgatcgagcg cgcgaccggc
     2821 aagcggccgg tgggctggat caatccgcgc ggcacgccga gcccgaatac cgcgcgcctg
     2881 ctggcgcagg agggcttcga atggcacggc gacaccttga atgacgattt gccctacctc
     2941 gtcagattcg gctcgaagtc catcgtggcg ctgccgagca cgatggaggt caatgatttg
     3001 ccgctgacca tgaagcacgg ccagccgccg agcgtttatg tcgagaactt cgaggcatgg
     3061 ctcgagtacg cgcgcaagcg cgagaagggt gcctgcaaga tcgatcccac ggtgcatgcc
     3121 cacgtcttcg ggcgtcccgc cggcacttgg gcgttcgagc gcgtcatgga gatcgcgaga
     3181 aaatcgaagg acatatggat cggcacgcgc gccgaggtgg cggcgcacgt gcgcaaggtg
     3241 ctcaagaacc cgtgattcgt gagtggcgag tcgtgagtcg aagggaaacc gcagggtcga
     3301 ttcacgattc acgctttttc gagaaaggaa gatagaaatg aggcttgtga cttttgtgcc
     3361 caggaagggc gccgcaacgc gcgtgggagt cgtgcgagat ggcaagcagg tgatcgatct
     3421 cgtcgaggcc ggcggcaagc cgccgttcga ccccgcggac atgctgtcgc tgatcgctgc
     3481 cggcgccaag gcgctgaagt ggacagatgg cgtggcggcc agggcgaaga aaaccctgcc
     3541 gctcgacaag gtgaagctcg cggcgccgat cccgcgcccg cgcaagaacg tgttctgcgt
     3601 cggctggaac tacctcgatc acttcgagga gggggcgaaa agccgcaccc agaaagtgga
     3661 cctgcccgag caccccgctt tc
//
LOCUS       DBNGWZ010000062         2036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_5245, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000062 DBNGWZ010000000
VERSION     DBNGWZ010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_5245"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16CX_01785"
     CDS             complement(<1..458)
                     /locus_tag="AC16CX_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011270.1"
                     /translation="MKTLERLRDHTRATDLREKVSFLSRPESYPEKPAAVEVVQTHMS
                     CVFLTERHAYKLKKPARYDFLDFSTLEARRLDCEEELRLNRRLAGDVYLELVPLTVTA
                     GGGLALGGEGEVVEWLLKMRRLPRRLMLDHAIREGSVDRHDVHRFALALA"
     gene            complement(515..997)
                     /locus_tag="AC16CX_01790"
     CDS             complement(515..997)
                     /locus_tag="AC16CX_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3011271.1"
                     /translation="MKVGQVCVRGVISASADTTIAKAAALMRDHHVGDLVVTENVDGR
                     RLPVGIVTDRDIVVGVVAADLDPDMMTLGNVMSGRLVTAYESQSVEEVLAIMRAAGIR
                     RLPIVSRDDELVGIVSLDDLLAVLASEMGDVVELIGRGQSREQASATAPLPLFSRTPG
                     "
     gene            complement(1474..1755)
                     /locus_tag="AC16CX_01795"
     CDS             complement(1474..1755)
                     /locus_tag="AC16CX_01795"
                     /inference="COORDINATES: protein motif:HMM:NF022603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HIR3011272.1"
                     /translation="MKTRHTGNTPVTPERVRPDELRVMRQAPKMRSRARAPAGANSTT
                     GISPEEFQQMVASAAYFRAEKRGFTAGHELEDWLEAETEIRRQLGQAAS"
     gene            complement(1752..>2036)
                     /locus_tag="AC16CX_01800"
     CDS             complement(1752..>2036)
                     /locus_tag="AC16CX_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011273.1"
                     /translation="QRSYRTRDGTALVAGHYVVLWNEAVETPGFDENATYVGPYKTSL
                     LASLMLRDFLRSLPMEGAAPADQERRLADIQADSGSGWLVTSAVEKEARL"
BASE COUNT          337 a          694 c          662 g          343 t
ORIGIN      
        1 tcggccagcg ccagggcgaa gcggtgaacg tcatgacggt cgacgctgcc ttcgcgtatc
       61 gcgtgatcga gcatgagccg gcggggcagc cggcgcatct tgaggagcca ttccaccact
      121 tcgccctcgc cccccagagc caatccgcct cccgccgtca cggtcagcgg cacgagctcg
      181 aggtagacgt cgcccgccag ccggcgattg aggcgcaatt cctcctcgca gtcgaggcgg
      241 cgggcctcca gcgtgctgaa atcgagaaaa tcgtagcggg ccggcttctt gagcttgtag
      301 gcgtgccgct cggtgagaaa tacgcaggac atgtgggtct gcaccacctc caccgccgcc
      361 ggcttctcgg gatagctttc gggacggctg aggaaagaaa ccttctcgcg caggtccgtc
      421 gcgcgggtgt ggtcccgcag ccgctcaagc gttttcatgc cagagatccg ccccgtgaca
      481 ggcaggggga gggtagaggg gaaaacccgg ctggttaccc cggcgtgcgc gagaagagcg
      541 gcaggggagc ggtcgcggat gcctgttcac gcgactggcc gcgcccaata agctccacca
      601 cgtcgcccat ttcggatgca agcaccgcga gcaggtcatc gaggcttacg atgccgacca
      661 gctcgtcgtc cctgctcacg atcggcaggc gccggatacc cgctgcccgc atgatcgcga
      721 gcacctcttc gacgctctgc gactcgtaag cggtgaccag cctgccgctc atgacgttgc
      781 cgagcgtcat catgtccgga tcgaggtccg ccgcgacgac accgacaacg atgtcgcgat
      841 cggtcacgat gccgaccggc aggcgcctgc cgtccacgtt ctcggtcacc accagatcgc
      901 ccacgtgatg atcgcgcatc aatgccgccg ccttggcgat agtcgtgtcg gcggaggcgc
      961 tgatgacgcc ccggacgcac acttggccca ctttcatggt cgcacctcct ttctccccgg
     1021 cgagcataga ctctcgcggg cctcgggtat tgatcaggat caagtcagat tcccgggcaa
     1081 aaaaatgcgc agcaaagcgc tgcgcatcaa acccacccaa gggaggtgcg ccgaaacgtg
     1141 gagcaggggc ccctgcttgt ggggatgcga ccgcggaggc gcacataagc acgcgggcca
     1201 ttgcggcgct gcatcccgct gcggggggcc ggtggaacgt ggagcagggg ccccgcttgc
     1261 ggggatgcga ccgtggaacc ggccgtgagc gcgtcgcatc cggccctatg cgcgtctccg
     1321 gggtcagatc cccagagggg cccctcttcc gccctacgac gcgccatcca agcacaatac
     1381 gcttagaacc tgctccgccc tccgcgggcg cagggtggga aggaggagcc ttgaccgcca
     1441 gcctccggcg gcggttgacg agccgcgggt ttactacgac gcggcctgcc cgagctggcg
     1501 cctgatctcg gtctcggcct cgagccagtc ctcgagctcg tgaccggcgg taaaaccgcg
     1561 tttttccgcg cggaaatagg ctgcgcttgc gaccatctgc tggaactctt ccggggagat
     1621 ccccgtggtg gaattggctc ccgcaggggc ccgcgcccgg cttcgcatct tgggggcttg
     1681 ccgcatcacg cgtaattcgt cgggccgcac ccgctcgggc gtaaccggtg tgttgcccgt
     1741 atgacgtgtt ttcatagtcg tgcctccttc tccacagcgg acgtcaccag ccagcctgaa
     1801 ccgctgtcgg cctggatgtc cgccagccgc cgctcctggt cggccggcgc ggcgccctcc
     1861 atcggcagcg agcgcaggaa atcacgcagc atcaggcttg ccagcaagga cgtcttgtac
     1921 ggcccgacgt aggtcgcgtt ttcatcgaat ccgggcgtct cgaccgcttc gttccacagc
     1981 acgacgtagt gtcccgcgac gagcgcagtg ccgtcgcgcg tgcgatagct gcgctg
//
LOCUS       DBNGWZ010000063         2569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2649675, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000063 DBNGWZ010000000
VERSION     DBNGWZ010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2649675"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="AC16CX_01805"
     CDS             complement(<1..548)
                     /locus_tag="AC16CX_01805"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3011274.1"
                     /translation="MQVDIPDPEYTASERARANFRLAGKLALGFVAVIWVIQVLNSGL
                     DLGLARFGVRPRELAGLPGILFAPLLHGSFAHLVANSVPLVVLGTGMLYLYPNSALKV
                     LPAVYLGPGIAVWLFAKASSVHAGASGLIFGLFSYIFVAGVIRRDRRAIAASLLVFFL
                     YGAMVWEVFPISPGVSWETHLAA"
     gene            786..>2569
                     /locus_tag="AC16CX_01810"
     CDS             786..>2569
                     /locus_tag="AC16CX_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR3011275.1"
                     /translation="MREKFTLRVAVASAVAAIILVTGSAAHAAPKKTLTAFASEQEIS
                     DLFKRWAEEHRRKQQARSAQAPQEMAPSAGMGAMMPAAPAAKMADKAAAEDSITNVQH
                     AGVDEGGIVKRHGDHLVILRRGRLFTVRIGDNDLTPVSAADAFGNGIDPRGAWYDEML
                     ISGDTIIVIGYSYARGGTEVGLFEISRAGRLRYKATYHLRSNDYYSSRNYASRLIGDK
                     LVFYTPMYLNPFGGEPWASFPALRRWRPGVTAADFKPIAPATRIYRTDEPLDPWQGVA
                     LHTVTVCDLAKAEFDCESTAVFGAPGRVFYVSGGSVYVWTTQWRRNRSTSVPGSALFR
                     IPLDGAAPTALKTAGSPIDQFSFLEGDDGHLNVLVRANGRGEAMWAAEWNAGDFALLR
                     VPLDSFTDGKDSAPAEAYKPLPRPNGHAIQNRYVGSYLLYGAGAGWYRAQRTLYSQVH
                     AVRYAGDAKTHEIPLAHGVDRIEALGANAIVVGSDGRDLHFTSVRLAATPMAQDRYTR
                     LDAAQGETRSHGFYYRAENDNAGIVGLPIIGGNHAASRQLRHESAAVLFLRNQALRLS
                     ELGALDSRAEANANDGCRASCVDWYGNSR"
BASE COUNT          454 a          911 c          862 g          342 t
ORIGIN      
        1 gccgcgagat gggtttccca ggaaacgccg ggactgatgg ggaacacctc ccacaccatg
       61 gcgccgtaga gaaagaacac cagcagggac gcggcgatcg cgcgccggtc gcgccggatc
      121 acgccggcga cgaagatgta gctgaacagg ccaaagatga gcccgctcgc gccggcgtgg
      181 acggaagagg ccttggcgaa cagccatacc gcgatccccg gccctaggta gaccgccgga
      241 agcaccttga gcgcagagtt cggatagagg tacagcatcc ccgtccccag caccaccagc
      301 ggcacggagt tggcgaccaa gtgggcaaaa ctgccgtgca gcagcggcgc gaaaagaatg
      361 ccgggcagcc cggcgagctc gcgcggccgc acgccgaagc gcgccagccc caggtccagc
      421 cccgagttca acacctggat gacccagatc acggcgacga aacccagcgc gagcttgccc
      481 gccagccgga agttggcgcg ggcccgctcc gaggcggtgt actccgggtc gggaatgtct
      541 acctgcatcg ggaacaagat actccgtcgc ccggcattca tcacccatta gcgtgactgg
      601 tgaccgggct ccgcgcggcg cgcgggagac tcatcaccca tcgctcatca cccatcaccg
      661 gtatttaacc cctcctcccc atctcccccg ttaataggcc ccgagcccgg caagaccagg
      721 gagagcgcag gcgtaactcc ctgatccggc cggtagatga ccattaaccg acaaggagca
      781 gggccatgcg ggaaaaattc acattgcggg tggcggtcgc gtccgccgtc gccgcgatca
      841 ttctcgtgac ggggagcgcg gcgcacgccg cgccgaagaa aacgctgacg gcgttcgcca
      901 gcgagcagga aatctcggat ctcttcaagc gctgggcgga ggagcaccgg cgcaaacagc
      961 aggcccgctc ggcgcaggcg ccgcaggaga tggcgccatc ggcgggcatg ggcgcgatga
     1021 tgcccgccgc gccggcggcc aagatggccg acaaggccgc ggccgaggat tcgatcacca
     1081 acgtgcagca cgccggcgtg gacgagggcg gcatcgtcaa gcgccacggc gaccatctcg
     1141 tgatcctgcg gcgcgggcgg ctcttcaccg tccggatcgg cgacaacgat ctcacgccgg
     1201 tgtcggcggc cgacgcgttc ggcaacggca tcgatccgcg cggcgcctgg tacgacgaga
     1261 tgctgatctc gggcgacacc atcatcgtga ttggctacag ctacgcgcgc ggcggcaccg
     1321 aggtcggcct gttcgagatc tcgcgcgccg ggaggctgcg ctacaaggcc acctaccacc
     1381 tgcgctcgaa cgactattac tcctcgcgca attacgcgag ccggctgatc ggcgacaagc
     1441 tcgtgttcta cacgccgatg tacctcaatc ccttcggcgg cgagccgtgg gcgtcgtttc
     1501 cggcgctgcg ccgctggcgg cccggcgtca ccgccgcgga cttcaagccg atcgcgccgg
     1561 cgacgcgcat ctaccgcacc gacgagccgc tcgacccctg gcagggcgtg gcgctgcaca
     1621 cggtcacggt gtgcgacctc gcgaaggccg agttcgattg cgagagcacc gcggtgttcg
     1681 gcgcgccggg gcgcgtgttc tacgtctcgg gcggctccgt ctacgtgtgg acgacgcagt
     1741 ggcgccgcaa ccgctccact tccgtgccgg gctcggcgct attccgcatc ccgctcgacg
     1801 gcgccgcgcc caccgcgctc aagaccgccg gcagcccgat cgaccagttc tccttcctcg
     1861 agggcgacga cggccatctc aacgtgctgg tccgcgcgaa cggccgcggc gaggcgatgt
     1921 gggccgcgga atggaacgcc ggggatttcg cgctgctgcg ggtgccgctc gacagtttca
     1981 cagacggcaa ggacagcgcg ccggcggaag cctacaagcc cctgccgcgt cccaacggcc
     2041 acgcgatcca gaaccgttac gtcggctcgt atttgctcta cggcgcaggc gcgggctggt
     2101 accgcgcgca acgcacgctc tactcgcagg tgcacgcggt gcgctacgcg ggcgacgcga
     2161 agacgcacga gatcccgctc gcgcacggcg tggaccgcat cgaggcgctc ggcgcgaacg
     2221 cgatagtagt cggctccgac ggccgcgacc tgcacttcac cagcgtgcgc ctcgcggcga
     2281 cgccaatggc gcaggaccgc tacacgcgcc tcgacgcggc gcagggcgag acgcgcagcc
     2341 acggcttcta ttacagggcg gagaacgaca acgccggcat cgtcggcctg ccgatcatcg
     2401 gcggcaacca cgccgcgtcg cgccagctgc ggcacgaatc cgccgcggtg ctgttcctgc
     2461 gcaaccaggc gctgcggctg agcgagctgg gcgcgctcga ctcgcgcgcc gaggccaacg
     2521 cgaacgacgg ctgccgcgct tcgtgcgtgg actggtacgg caactcccg
//
LOCUS       DBNGWZ010000064         1849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1894384, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000064 DBNGWZ010000000
VERSION     DBNGWZ010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1894384"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1849
                     /locus_tag="AC16CX_01815"
     CDS             <1..>1849
                     /locus_tag="AC16CX_01815"
                     /inference="COORDINATES: protein motif:HMM:NF006196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIR3011276.1"
                     /translation="STLVLHGGGNTSVKIRERNLVGEQEDILYIKGSGCDLETIEEAG
                     FAPVRVAHLRKLARLEALTDPQMMNEFATQVTRAGAPTPSVEVLLHALLPHKFVDHTH
                     ADAVLSVANTADGEKHIREIYGDQAVVIPYVMPGFDLARACAVKFPTEAGANTIGMVL
                     MKHGVFSFGRSARESYERMIQLVQFAEDYLSRRRAWSLPKPLDDAAGKPPRHDLATLR
                     RDLSTAAGFPLVMRCHSDARCISFARRLGAREIAPGPITPDHVILTKRLPMIGRDVAA
                     YAQAYRDYFSEHAPRAKEPKTMLDPAPRIVLDPELGLCAAGRTAKDAAIASDLYRHTM
                     DVIERAQLLGGYRALPAGDIFDVEYWDLEQAKLKKAGTPPVFSGEVALVTGAASGIGK
                     ACVEALLKRGAAVIGLDINPGVAQLMGSRVDFLGLQCDVSSERDVTAALEAGVRVYGG
                     LDMLVLNAGVFPGGRRIEALASDEWRRVMRINLDANLALMRECHPLLKLAPRGGRIVV
                     IGSKNVPAPGPGAAAYSASKAALTQLARVAALEWGADSIRVNTLHPNAVFDTAAWTDE
                     VLAARAGHYGMTVEEYKMKNVLKVEVRSRDVAELAAEMCGPLFARTTGA"
BASE COUNT          321 a          605 c          632 g          291 t
ORIGIN      
        1 attcgacgct ggtcctgcac ggcggcggca acacatcggt caagatccgg gagcgcaatc
       61 tcgtcggcga gcaggaagac atcctctaca tcaagggcag cggctgtgac ctcgagacca
      121 tcgaggaggc cggcttcgcg ccggtgcgcg tggcgcacct gcgcaagctc gcgcggctcg
      181 aggcgctcac cgatccgcag atgatgaacg agttcgccac ccaggtgacg cgcgccggag
      241 caccgacgcc ttcagtcgag gtcctgttgc acgcgctatt gccgcacaaa ttcgtcgacc
      301 atacgcacgc ggatgcggtg ctgtcggtcg cgaacaccgc cgacggcgag aagcacatcc
      361 gggagatcta cggcgatcag gcggtcgtaa tcccctacgt catgccggga ttcgatcttg
      421 cgcgagcgtg cgcggtgaag tttcccacgg aagccggggc caacaccatc ggcatggtgc
      481 tgatgaagca cggtgtcttt tcgttcggta ggtccgccag ggaatcgtac gagcgcatga
      541 tccagctcgt gcagttcgcc gaagactacc tgtcccggcg gcgcgcttgg tcgttgccga
      601 aacctctgga cgatgcggcc gggaaaccgc cgcgccacga tctcgccacg ctgcgccgcg
      661 acctatcgac cgccgccggg tttccgctgg tgatgcggtg ccattccgac gcccgctgca
      721 tcagcttcgc gcgccgcttg ggcgcccggg agatcgcacc gggaccgatc accccggacc
      781 acgtcatcct caccaaacga ctgcccatga tcgggcgcga cgtcgcggcc tacgcgcagg
      841 cgtaccgcga ctatttcagc gaacacgcgc cgcgggcgaa ggaaccgaag accatgctcg
      901 atccggcgcc acgcatcgtg ctcgatcccg agctcggcct gtgcgccgcg ggacgaacgg
      961 cgaaggacgc ggcaatcgcg agcgatttat acaggcacac gatggacgtc atcgagcgcg
     1021 cgcagctgct tggcgggtac cgtgccctgc cggccggcga catcttcgac gtcgaatact
     1081 gggacctcga acaggccaag ttgaagaagg cgggaacgcc accggtgttt tcgggagagg
     1141 tcgcgctcgt aaccggcgcg gcttcgggaa tcggcaaggc ctgcgtggag gcgctgttga
     1201 agcgcggcgc ggcggtgatc gggctcgaca tcaatcccgg tgtcgcgcag ctgatgggct
     1261 cgcgcgtcga tttcctcggc ctgcaatgcg acgtgtcctc ggagcgggac gtgaccgccg
     1321 cgctggaagc cggcgtgcga gtctatggcg gcctcgacat gctggtcctc aacgcgggcg
     1381 tcttccccgg cggccggcga atcgaggcgc tcgcctcgga tgaatggcgc cgcgtgatga
     1441 ggatcaatct cgacgccaac ctcgcgctca tgcgcgagtg ccatcctctg ctcaagctcg
     1501 cgccgcgcgg ggggcgcatc gtggtgatcg gctccaagaa cgtgccggcg ccgggcccgg
     1561 gcgcggctgc gtattcggca tcgaaagctg cgcttaccca gctcgcgcgc gtggctgctc
     1621 tggagtgggg cgcggacagc atccgcgtga atacgctgca ccccaacgca gtgttcgaca
     1681 cggcggcctg gaccgacgag gtgctggccg cgcgtgccgg tcactacggc atgaccgtcg
     1741 aggaatacaa gatgaaaaac gtgctcaagg tggaagtgag aagccgggac gtggccgagc
     1801 tcgcggcgga gatgtgcgga ccgctgttcg ccaggaccac cggcgcgca
//
LOCUS       DBNGWZ010000065         3574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2776283, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000065 DBNGWZ010000000
VERSION     DBNGWZ010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2776283"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC16CX_01820"
     CDS             <1..270
                     /locus_tag="AC16CX_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011277.1"
                     /translation="ANPPPVTISINGREVGKIGSRWRGSNPPRTEVEIYSDIERIYGK
                     KGRQSAAAGEHFVHNFRHAAAFGRPDGTVVLRGRGGRRLWDYFQA"
     gene            509..1156
                     /locus_tag="AC16CX_01825"
     CDS             509..1156
                     /locus_tag="AC16CX_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011278.1"
                     /translation="MARRRYRNARRVVVVNAPRRRRRRTVYRRRRYRHNPHRYRRNDP
                     ARRRRYYRHNDPARRRYYRRNAPRTAVQLTRPMTWLPLLLTGGAAATVSSILPRLILG
                     AGVTTNQEYAVQAGVALGGAFVLPMLGLRGAHPIAWMVGAAAPIVAKFVTDRVAEWLG
                     LGWYPYQALQQSPFYPPRLYGADMYPYEGGQVMWDYQPQERVGAPEAPFEHMYNT"
     gene            1279..1989
                     /locus_tag="AC16CX_01830"
     CDS             1279..1989
                     /locus_tag="AC16CX_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011279.1"
                     /translation="MNGNMAVPVAPAVPADPGQFAKYRTQMRESPEDIWQPIYDRKNY
                     PAAGTFELIYFSIPVGGTDTLIRAGAAASVTKTRRDTNLEQQGVIPTKAFKIHGFSLC
                     FIPVQQAASATGVSGAASTPSILDDMMRLMYGGFMEFRIVDKPYLYLPLHKIPATGVL
                     RGTAFAAGIPAGGIASVSAGGPGTGAPRDIYWIGIPLVLDPYQNFSLRLQFDGSPPTN
                     MTWDIQIFLEGYLRRPGQ"
     gene            complement(2011..2559)
                     /locus_tag="AC16CX_01835"
     CDS             complement(2011..2559)
                     /locus_tag="AC16CX_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011280.1"
                     /translation="MSNRWKTEGTLVAHGLRGYLAGILDGEGSIVAHISRSGPKDARA
                     KGRRYWGITVQVKMTNKAVIELLQREYGGHIQIIVEKGARAHFRTLYGWRALNRHVVP
                     VLKDTMPWLLIKREQAQAAIRLAELSARVEKGRAGLNNVSAVREKQRLIQRIVELNGR
                     KAPPVFRGDTLYVVEPGHIPLS"
     gene            2632..>3574
                     /locus_tag="AC16CX_01840"
     CDS             2632..>3574
                     /locus_tag="AC16CX_01840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3011281.1"
                     /translation="MGKAILWISGRFDGCWWYRVNLPRRWLVARGYDVRCVEGDGGQD
                     VGHIGDFGAMVLQRVSYKAANEAVTMMALMRELRARGASVYYEIDDDLWNNSFLKTSG
                     PFFMPRPVRRAVMRDAERIIERCSGVIVTTPDLGDKLKHYNRRVIVIPNAVPTELCEV
                     ESRRPHAGVRIGWSGSRSHGLEGDFDRALPALRAVLAARPEVQLVFMGWHPPEVEAWP
                     RTEWSGWGPVQDYYARLAALDLDIFIAPLAESTFNRGKSPSKLLEAAALGLAIITEPH
                     GPYRNALQDDCTALYVQNVPEWQAALLRLVDEPETRRR"
BASE COUNT          587 a         1230 c         1153 g          604 t
ORIGIN      
        1 gccaacccgc cgcccgttac aatcagcatc aatggccggg aggtcggaaa gatcggctcg
       61 cgctggcgtg ggtcgaaccc tccacgcacc gaggtcgaaa tctactccga catcgagcgc
      121 atctacggca agaagggacg gcagagcgcc gccgcgggcg agcatttcgt ccacaacttc
      181 cgccatgccg cagccttcgg ccgaccggac ggcacggtcg tgctgcgtgg gcgcggcggg
      241 cgcagactgt gggactactt ccaagcgtaa ggaggacgac gccatgtggg agccctactt
      301 catcaacccg cgcggtcagc gcgtgagtac ccgccgtcgg cgtcgctctc gcaacccacg
      361 tgccgtgcgc gatccgcgca cgggccggtt ccgccgtcgc ggtcgcggct accggcgcaa
      421 cgatccactg accgtacttg gcctcaatcc acgccgccgc cggaggaacc cggcccggcg
      481 ccgtcgaggg ggcacgagga ggacacccat ggcacgtcgc agataccgca acgcgcgccg
      541 ggtggtggtg gtcaacgccc cccgtcgccg gcgtcggcgc accgtctacc gccgccgccg
      601 ctaccgccac aacccgcacc gctaccgccg gaacgatccg gcgcgccggc gtcggtacta
      661 tcgccacaac gacccggccc ggcggcgcta ctaccgccgg aacgcgccgc ggacggccgt
      721 gcagctcacg cggccgatga cgtggctgcc gctgctgctc accggcggcg cggcggcgac
      781 cgtctcgtcc atcttgccgc ggctcatcct cggcgctggc gtgacgacca atcaggaata
      841 tgctgtccag gccggcgtcg cgctcggcgg cgccttcgtg ctgccgatgc ttgggctgcg
      901 cggggcgcat cccatcgcgt ggatggtcgg cgccgcggcg ccgatcgtgg ccaagttcgt
      961 gaccgaccgg gtcgcggagt ggctcggcct cggctggtat ccgtaccagg cccttcagca
     1021 gtcgccgttc tatccgccgc ggctgtacgg cgccgacatg tacccctacg agggcgggca
     1081 ggtgatgtgg gactaccagc cccaggagcg cgtcggcgcg ccggaggcgc ccttcgagca
     1141 catgtacaac acctagcgcc gcatcggcgc tgggaccgcg cggcacgggc cgcgcttggc
     1201 atcaacctcg agccccgaaa cgcgagcctc tccgaaggcc agggatgagg gccgaagcac
     1261 cgagagagga tcgcgcgcat gaacggcaac atggcagtcc ccgtcgcgcc ggccgtcccg
     1321 gccgaccccg ggcagttcgc caagtaccgc acccagatgc gggagtcccc ggaggacatc
     1381 tggcagccga tctacgaccg gaagaactat ccggccgcgg gtaccttcga gctgatctac
     1441 ttctccatcc ccgtcggcgg cacggacacg ctgattcgcg ccggcgccgc agccagcgtc
     1501 accaagacgc ggcgggacac gaaccttgag cagcagggcg tgatcccgac caaggccttc
     1561 aagatccacg gtttctcgct ctgcttcatc cccgtgcagc aggcggcctc ggccaccggc
     1621 gtgagcggcg ccgcctcgac gccctcgatc ctggacgaca tgatgcggct gatgtacggc
     1681 ggcttcatgg agttccggat cgtcgacaag ccgtacctct acctgccgct ccacaagatc
     1741 cccgcgaccg gcgtgctgcg cggcacggcc ttcgcggcgg gcattcccgc cggcggcatc
     1801 gcctccgtct ccgctggcgg tcccggcacc ggggctccgc gcgacatcta ctggatcggc
     1861 atcccgctgg tcctcgaccc gtaccagaac ttctcgctgc gcctgcagtt cgacgggtcg
     1921 ccgccgacca acatgacctg ggacatccag atcttcctgg aagggtattt gcggcgaccg
     1981 ggtcagtgat tctgctgtat tttcactgac ttacgaaagg ggtatatggc ccggctcgac
     2041 aacgtagagc gtatcgccgc gaaagaccgg aggagccttg cgcccgttga gttccacgat
     2101 gcgctggatc agccgttgtt tttcacggac cgcgctcacg ttgttgagcc cggctctgcc
     2161 tttttcgacg cgagcagaaa gctctgccaa gcggattgcc gcttgagcct gctctctttt
     2221 gatgagcagc cagggcatcg tgtccttgag taccggaacg acatggcgat taagagcccg
     2281 ccagccgtaa agagtacgaa aatgggccct agcccctttc tcaacaatga tttggatgtg
     2341 tcccccgtac tcccgttgaa gcagttcgat aaccgccttg tttgtcattt tcacttgcac
     2401 agtaatgccc caatagcggc gcccctttgc gcgagcatcc ttagggccag atcggctgat
     2461 gtgcgcgaca atgctgccct ccccatcgag gatccccgcc aagtagccgc gcaggccatg
     2521 ggccaccagc gtgccctcgg ttttccagcg attgctcatt gtgggcctcc tgtacccatt
     2581 atagacggat atgcgaggcc ttgcattcat tctgttggag gcgtctaggc catgggcaag
     2641 gccattctct ggatctcggg ccgcttcgac ggctgctggt ggtatcgcgt caacctgccg
     2701 cgccgctggc tggtggcgcg tggttacgat gtgcgctgcg tggaaggtga tggggggcag
     2761 gatgtgggcc acatcggtga cttcggcgcg atggtgctcc agcgagtgag ctacaaagcg
     2821 gcgaacgaag cggtcacgat gatggccctc atgcgcgagc tgcgcgcccg tggggccagc
     2881 gtgtactacg agatcgacga tgacctctgg aataatagtt tcctcaagac tagcggcccc
     2941 ttcttcatgc cgcgtccagt gcgccgcgct gtcatgcgcg acgctgagcg catcatcgag
     3001 cgctgctctg gcgtgatcgt gacgacgccg gatctcggcg acaaactcaa acactacaac
     3061 cggcgcgtca tcgtcattcc caatgccgtg ccgacggaac tctgcgaggt tgagtcgcgg
     3121 cggccccacg cgggcgtgcg catcggctgg agcggcagca ggagtcacgg gcttgagggc
     3181 gatttcgacc gggccctccc cgcgctgcgg gcggtgctcg cagcgcggcc cgaggtccaa
     3241 ctcgtcttca tgggttggca cccgcccgag gtcgaggctt ggccgcgcac ggagtggagc
     3301 ggatggggac cggtccagga ctattacgcg cgcttggcgg cgctcgacct cgacatcttc
     3361 atcgcgccgc tggctgagtc caccttcaac cgtggcaaga gcccgagcaa gctgctcgaa
     3421 gccgccgcgc tcggcctagc gatcattacc gagccgcacg ggccgtaccg taacgccttg
     3481 caggacgact gcacggcgct ctatgtgcag aacgtgccgg agtggcaggc cgcgctgctg
     3541 cgcttggtcg acgagcccga aacgcgccgc cgct
//
LOCUS       DBNGWZ010000066         4214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1894525, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000066 DBNGWZ010000000
VERSION     DBNGWZ010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1894525"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1157
                     /locus_tag="AC16CX_01845"
     CDS             225..1157
                     /locus_tag="AC16CX_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011282.1"
                     /translation="MSDEYKYDVAFSFLQRDEPLAMQLNDRIQDRVNTFIYTEQQKVV
                     AGTDGEETLHRVFGKEARVVVVLYRMGWGESRWTRIERTAIRNRGHERGYTFAVFVPL
                     DEPPNVPDWLPAAQIWVDFNRFGIEGTAAVIEARIQDAGGQVHQETLEEHAQRTKRAI
                     EFAKRREKFQWDEGVRMAQQEVAKLKLALESRAEIVKNSSSIQLSVQHTRWGSVYLIG
                     LGCALGVLWKQQVINHLRDAFLDVALFTHRPAGPGEFQFETPQKIHSMRFVFDLLPSE
                     MGGWISDDAQRRSFSTDALAEYLTKFYLEHGRKA"
     gene            2099..2584
                     /locus_tag="AC16CX_01850"
     CDS             2099..2584
                     /locus_tag="AC16CX_01850"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR3011283.1"
                     /translation="MAQQIKILFVCMGNICRSPMAEGVFKHYVKEAGLAERIVSDSAG
                     THDYHIGEPPDPRAQRAAGRRGYDLGTLRGRQVSRNDFGGFDYVLAMDEMNLRLLKRL
                     CPPQHVHKLMLFMEFAADPDLREVPDPYYGGAEGFERVLDMAEQAAQGLLQHLRERLP
                     G"
     gene            complement(2748..>4214)
                     /locus_tag="AC16CX_01855"
     CDS             complement(2748..>4214)
                     /locus_tag="AC16CX_01855"
                     /inference="COORDINATES: protein motif:HMM:NF044325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011284.1"
                     /translation="RPSLGETAHSPCPRCHGTGHIRGTESTALHILRIIQEEAMKENT
                     GAINAQVPVDVATFLLNEKRVDLHLIEARHRVSVMLIPNMHLETPNYTVTRLRHDDLN
                     KTEPLPPSYNLVELPAEEQKPAAGAVETAAPRQEAAVKGITPQQPAPVPVAREPRKEP
                     PQPTTAPAGEGSIIGKIFGWFKRKPTETPRAAQTAQQVPERGPRRGRHERGEREGGRR
                     DREPERAMQDGQRQRPDATQPGRQRDSGRQRYEQERRGERRPEFRRQEQDQPQRQQQP
                     QRGPMPGDAGKPAQGQQQPREHGEQREGRGRRRRRGRDRHEQRQMEAGGGQPAHGQQA
                     QREPQRLPSEHPSPDTVSAVPLAPVTPADAMTGAPVSVPSYKAPPDAAFETEEVREFQ
                     PREPVPMAAPSGPGYAPAEPVRLEWPSDLQQVESDPDKVRAAQQEVPQEQPAPRPKRV
                     RQPLPPVSEEPLVQIETSQPEAASADAEQKEKETALPG"
BASE COUNT          794 a         1240 c         1346 g          834 t
ORIGIN      
        1 cttctgcatc atcgcccgca agatcgcacg cacggcatgg tcgatcttta agtaccaaac
       61 cacctttgat ccgcaacgcc tcacctgcca gccttgacca gaaccataga atctctcagg
      121 ggccccaaga tcaaaatcca tcatcgctca gtcgtgactg gtcatcagcg actcgtcacc
      181 gtatcattct tgggatttca ccatcaggct tgagggaggc ggcgatgagc gacgaatata
      241 agtatgacgt cgctttctca ttcctgcagc gcgatgagcc actcgccatg cagctcaatg
      301 accgaattca ggaccgggtc aatacgttca tctacaccga gcagcagaaa gtcgttgctg
      361 ggacggacgg ggaggaaacg cttcaccgcg tttttggcaa ggaagcgcga gtagtcgttg
      421 tcctatatcg catggggtgg ggcgagtcgc gctggacccg catcgagcgg acggcgatcc
      481 gaaatcgtgg tcatgagcgc gggtatacct tcgcggtttt tgtcccgctc gatgagcccc
      541 cgaacgtgcc tgattggctg cctgccgccc agatatgggt tgacttcaat cgattcggga
      601 tagaagggac agcggcagtc attgaggcgc gaattcaaga cgctggggga caggtccacc
      661 aggaaaccct tgaagaacac gctcaacgaa ccaagcgcgc aatagagttc gcgaagcgac
      721 gcgagaaatt tcagtgggac gaaggcgtac gaatggcgca gcaggaagtc gctaagctca
      781 agctggctct agaatcgcga gccgagattg taaaaaactc atcatcgatc cagttgtcgg
      841 tgcaacacac gcggtgggga tcggtctact taatcgggct cggatgcgca ttgggggtcc
      901 tgtggaagca gcaagtaatc aatcatctcc gagacgcctt tcttgacgtg gccttattca
      961 cccatcggcc agccgggcca ggtgaatttc aattcgagac tccacaaaag atacattcta
     1021 tgcgcttcgt gttcgatcta cttccttcag aaatgggggg atggatctcg gacgacgccc
     1081 agcggcgaag cttctcgacg gacgcactcg ccgaatacct cacgaagttc tatcttgaac
     1141 atgggaggaa agcatagcta ccagtgactc gcgaaggccc aaggctgacg agcttataaa
     1201 aatcagcctc gacgatcagg gccatgtctt cacaaccaca gatgagttgt aacaggtgcg
     1261 tgacgggtgg gcgcttgcga tcgggcgatt catagtggcc ttcgcaggct gtgaatactg
     1321 gacatatctc ttcgtcagga cctacggtac tgctgcggag cgcgaggcga taaataaaca
     1381 acttctcagg gagaggctta ctgttctcgg gaaagtcgta ttaaggatgc acctaacgcc
     1441 cgagatgcag cagcaagtta acgcagcgac aaaacgattt cgccgactcg tgccgacccg
     1501 taacattctg gcacacaatg cccctatggt tcgcgtttat gttcacgagg taaccggcaa
     1561 gttcgaagtg aagcacgaat tgcgatgcgc tcgcgactct tcgaaaggac ttaccgcaga
     1621 gcgaattgag caggaagctg ccgaagcgga aaagattgaa gaggaattcg cactccttta
     1681 tggccaagtc cgaaagccag aaagccgatc aagaacgcca agaggacaat gatttcttcc
     1741 ccatcaaggg ggaagggcca gggagggggt ggttcatggg gcccctgaga gccgcccgcg
     1801 cagcgcagac cggcccgggg tttcgggcga gcactgggcg agttgggaac gtgctggcag
     1861 gatcgcagga aattcgtgcc ttatgagtct gcaggagtca tggctctgcg cgagttgcgc
     1921 agaccgccgg gacggtcgag cagcacaggg gaaccgaaaa tccgggacag acccaaaccc
     1981 caggtctgtc ccggttttcg gcggcgaacc ggggaacccc tttctttcgt gactttcttt
     2041 gggggagcaa agaaagtcac tcgcccttca gggcgaaacc cgaattaaaa tcattttgat
     2101 ggcgcagcaa atcaaaatcc tgttcgtgtg tatgggcaac atctgccgtt cgccgatggc
     2161 ggaaggggtg ttcaagcact acgtgaagga ggccgggctg gcggagcgca tcgtgagcga
     2221 ctcggcgggc acgcacgact atcacatcgg cgagccgcct gatccgcgcg cacaacgtgc
     2281 tgcaggccga cgaggctatg atctagggac gctgcgcggg cgccaggtaa gccggaacga
     2341 cttcggcggg ttcgattacg tgctggcgat ggacgagatg aacctgcggc tgctgaagcg
     2401 cctgtgcccg ccgcagcacg tacacaaact gatgctcttc atggaattcg ccgccgatcc
     2461 tgacctgcgt gaagtccccg acccgtatta cggcggcgcg gaaggattcg agcgcgtgct
     2521 cgacatggcg gaacaggcgg cgcagggcct gctccagcac ttacgggaac ggctgcccgg
     2581 gtaagcgcgg atcatgtgtc gacgacccaa aggatgaagg atgaaggatg agggatgaag
     2641 aatgaccccc gcagtgcggg agcagtattt agctcagcct tcatccttcc gccttcatcc
     2701 ttcaggcggg cctatccgcg agccgctatc cgcgagtcac ggaagtacta gccgggcagg
     2761 gcggtctctt tctctttttg ttcagcgtcg gcggatgccg cttccggctg gctggtctcg
     2821 atctgaacca gcggctcctc gctcaccggc ggcaacggct ggcgcacgcg cttcgggcgc
     2881 ggcgctggct gctcctgcgg aacctcctgt tgcgcggccc tgaccttatc ggggtcggac
     2941 tcgacctgtt gcaggtccga tggccattcg agcctgaccg gttctgccgg ggcgtaaccg
     3001 gggccggaag gcgccgccat cggaactggt tcacgcggct ggaattcgcg tacttcctcg
     3061 gtctcgaatg cggcatcagg cggcgccttg taggaaggca cgctgacagg agcacccgtc
     3121 atggcgtccg ccggagtcac cggggcgagc gggacagcgg aaaccgtatc cggtgaggga
     3181 tgctcggatg gcagcctttg gggctcgcgt tgcgcctgct ggccgtgtgc gggttggccg
     3241 ccgccggctt ccatctgccg ttgctcgtgc cggtcacgtc cccggcgccg ccttctgccg
     3301 cggccttcgc gttgctcgcc atgctcgcgt ggctgctgct gaccttgggc gggcttgccc
     3361 gcgtcacccg gcatgggacc acgctgcggt tgctgctggc gctgcggctg gtcctgttcc
     3421 tgcctgcgga attccgggcg tcgttctccg cggcgctcct gctcgtagcg ctggcgcccg
     3481 ctatcacgct gcctgcccgg ttgcgtggca tccggccgtt ggcgctggcc atcttgcatc
     3541 gcgcgttccg gttcgcggtc gcggcgcccg ccctcgcgtt ccccgcgttc gtgccggccg
     3601 cggcgcggtc cgcgttccgg gacctgctgc gcagtctgcg cggcccgagg tgtttcagtg
     3661 ggcttgcgct tgaaccagcc gaaaatcttg ccgatgatcg atccttcccc ggcgggcgcc
     3721 gtcgtcggtt gcggcggctc ctttcggggt tctctcgcca ctggcaccgg cgccggttgc
     3781 tgcggcgtga tgcccttgac cgccgcttcc tggcgcgggg ccgccgtctc gaccgcgccc
     3841 gcagccggct tctgctcctc cgcgggcaat tccacgaggt tgtagctggg cggcagcggc
     3901 tcggtcttgt tgaggtcatc gtggcgcagc cgggtcacgg tgtaattggg ggtctcgaga
     3961 tgcatgttcg gaatcagcat gaccgaaacc cggtggcgcg cctcgatgag atgcaggtcc
     4021 acgcgcttct cgttgagcag gaaggtcgcg acgtccaccg gcacctgggc gttgatcgct
     4081 ccggtattct ccttcatcgc ctcttcctgg atgatgcgca ggatgtgcaa cgcggtggat
     4141 tccgtgccgc ggatatggcc ggtgccgtgg cagcgcgggc acgggctgtg cgcggtttcg
     4201 ccgagcgagg ggcg
//
LOCUS       DBNGWZ010000067         2303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2902333, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000067 DBNGWZ010000000
VERSION     DBNGWZ010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2902333"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1023
                     /locus_tag="AC16CX_01860"
     CDS             130..1023
                     /locus_tag="AC16CX_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3011285.1"
                     /translation="MVSKGRQFKELINAPEILVQPGIYDGYSARLVEKAGFRTASISG
                     AGVSESRLGWADRGIMTLEDNLANARRLADCTDLLLRADADTGYGNAINVYFTVRAFE
                     KAGMAALMFEDQVWPKRCGHMAGKSVIPAEEMVQKVKAAVDARRDPDFVIVSRTDAAG
                     PLGVDEAIRRLNLYAEAGADCLYADALLSKADIAKVAKNVPRPLIVNMGLGMRSHKTT
                     PLMTPKELQALGVAAVSYPRLLSTAALRGMMNAMAVFKEEVLEKNRVVDRPDLLVSFE
                     ELNDLMGMATLDELERRFIGG"
     gene            1084..>2303
                     /locus_tag="AC16CX_01865"
     CDS             1084..>2303
                     /locus_tag="AC16CX_01865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HIR3011286.1"
                     /translation="MGMTIAEKILARKSGQAKVGPGDLVTVEVDTVVLIDNSFTASRW
                     REVLKLRDPARIIVVFDHRVPASTTDSAAAHRTGRRFVEKFGIKRFHDVGYHQGISHQ
                     LVADHGYALPGTVLVCSDSHTCSAGVFNCIARGVGEPDVIYAAIKGETWFRVGETVRY
                     ELEGKLSRAVTAKDAFLQIAGQYGDHATQNVEFGGPGIADLSIAARKTLTTMGAELSA
                     EFATFEADDVMIEWLRARNPQPIEPVAPDQDAKYAAVRKVNLSALEPLVAFPDTVIEN
                     SRPVAEAAGTPIDQAFIGSCANGTLDDLELAAKVLDGRRVAPTTRLIVTPGSQTVYRE
                     AARLGILTTLVEAGAVVTPSTCGACGGGHLGILGPNETCITASTRNFKGRMGDPSAKI
                     FMGSPATVAASAIRG"
BASE COUNT          417 a          726 c          799 g          361 t
ORIGIN      
        1 cgcgacgacc tggcgaaatt caagcgcatc atcgacgagc ggcggctgac ctttccggag
       61 tagcgagagt gtcatgagcg aattcgccgt cgtatagaat ccatactcgc cacgcaacgg
      121 agcatcccca tggtcagcaa aggccgccag ttcaaggaac tcatcaacgc gcccgagatc
      181 ctggtgcagc cgggcatcta cgacggatac agcgcgcggc tggtggagaa agcgggcttc
      241 aggaccgcct cgatttcagg cgctggcgtg tcggagagcc ggctcggctg ggccgaccgc
      301 ggcatcatga ccctcgagga caacctcgcc aacgcgcggc gcctcgccga ctgcacggac
      361 ctgctgctgc gggcggacgc cgacaccggc tacggcaacg cgatcaacgt ctacttcacg
      421 gtgcgcgcgt tcgagaaggc gggcatggcg gcgttgatgt tcgaggacca ggtctggccc
      481 aagcgctgcg gccacatggc ggggaagagc gtgatccccg cggaggagat ggtgcagaag
      541 gtcaaggccg cggtggatgc gcggcgcgat cccgatttcg tgatcgtttc gcgcaccgac
      601 gcggcggggc cgctcggcgt ggacgaggcg atccggcggc ttaatctcta cgcggaggcg
      661 ggggcggatt gcctctacgc tgacgcgctg ctgtcgaaag cggacatcgc gaaagtggcg
      721 aagaacgtcc ccaggccgct gatcgtcaac atggggctcg gcatgcgctc gcacaagacg
      781 acgccgctga tgacgccgaa ggagctgcaa gcgctgggtg tggcggcggt aagctacccg
      841 cggctgctct cgacggcggc gctgaggggc atgatgaacg caatggcggt gttcaaggag
      901 gaggttctcg aaaagaaccg cgttgtggat cgtcccgacc tgctggtgtc cttcgaggag
      961 ctcaacgatc tcatgggcat ggcgacgctc gatgagcttg aacgtcgttt catcggggga
     1021 taagcattat gtgctgccga cacgacgagg tggaatcttg agatacgcta acggaggcgg
     1081 cacatgggca tgacgattgc cgaaaagatc ctggccagga agagcggcca ggcgaaggtg
     1141 gggccgggcg atctcgtgac cgtcgaggtg gacaccgtgg tcctgatcga caacagcttc
     1201 accgcgagcc ggtggcgcga agtcctcaag ctccgggatc cggcgcggat catcgtcgta
     1261 ttcgaccacc gtgtcccggc ctcgacgacg gacagcgcgg cggcgcaccg gacgggccgc
     1321 cgcttcgtcg agaagttcgg catcaagcgt tttcacgacg tcggctatca ccagggcatc
     1381 agccatcagc tcgtcgcgga ccacggttac gcgctgcccg gcaccgtgct cgtgtgcagc
     1441 gactcacaca cgtgcagcgc gggcgtgttc aactgcatcg cgcgcggcgt cggcgagccc
     1501 gacgtcatct acgcggcgat caagggcgag acgtggtttc gcgtcggaga gacggtgcgc
     1561 tacgagctgg aaggcaagct ctcccgcgcg gtcacggcga aggatgcgtt cctgcagatc
     1621 gcgggccagt acggcgatca cgcgacgcag aacgtcgagt tcggcggccc tggcatcgcc
     1681 gacctttcga tcgccgcgcg caagacgctt acgacgatgg gggcggagct ttccgcggag
     1741 ttcgcgacct tcgaggccga cgacgtgatg atcgaatggc tgcgcgcgcg caatccgcag
     1801 ccgatcgagc ccgtagcccc ggaccaggac gccaagtacg ccgccgtgcg caaagtgaac
     1861 ctgtccgcgc tcgagccgct ggtggcgttt cccgacaccg tcatcgagaa ctccaggccg
     1921 gtggccgagg cggcgggaac ccccattgac caggcgttca tcggctcctg cgccaacggt
     1981 accctggacg acctggagct ggccgccaag gtgctcgatg ggcggcgcgt ggcgcccacg
     2041 acgcgtctca tcgtgacgcc ggggtcacag accgtttacc gcgaggccgc tcggcttggc
     2101 atcctcacca cgctggtcga ggccggcgcg gtggtgacgc cttccacctg cggcgcgtgc
     2161 ggcggaggcc acctcggcat cctcgggccg aatgaaacct gcatcaccgc gagcacgcgc
     2221 aacttcaagg gccgcatggg cgatccgagc gcgaagatct tcatgggttc gccggccact
     2281 gttgccgctt cggcaatccg cgg
//
LOCUS       DBNGWZ010000068         7909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2776410, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000068 DBNGWZ010000000
VERSION     DBNGWZ010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2776410"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16CX_01870"
     CDS             complement(<1..403)
                     /locus_tag="AC16CX_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011287.1"
                     /translation="MKGRKKTEAEISPLMREVAEYIASALKRPLPQEVVEKTKHHLLD
                     TIAAMVSGSRLLPGKKAIAYVKTLGGAREACVIGSRLVTSAQHAALVNGMLGHADETD
                     DSHAPSLCHPGCAIVPAALAMAEREKRNGTAF"
     gene            complement(464..1798)
                     /locus_tag="AC16CX_01875"
     CDS             complement(464..1798)
                     /locus_tag="AC16CX_01875"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011288.1"
                     /translation="MDVTRILARYLVTSRWEDIPERVRHEAARALLNWVGCAVGGCRH
                     ETVERALAALQPFCGPPQAAVLGRGERLDILHAALINGISSHVLDFDDTHARAIHPSA
                     PVLPAVLALAEWRRINGAKLLHAFVLGVEVEERVGLSVFPEHYNAGWHITGTAGVFGA
                     AAAAGKLLALDERQMGWALGIAATQAAGLREMFGSMCKSLHPGRAAQNGLTAALLAAR
                     GFTSSERAIEAPRGFARVSSPKFDPSVITEGLGERYEILSNMYKPYACGLVVHAAIDG
                     CIEICREHRLAPEAIEAVELTVSPLVLELTGNTAPLTGLEGKFSVYHAVSAAIVHGAA
                     GEAQFSDACVRDPRVVDLRGRVNTRVDPAIGRTEARVTIRTRDGRTFDRHVEHALGTL
                     ARPMSDADLEAKFKGLAEKVLPPAQVEETIHLCRRITALEDAGALARVAVPQ"
     gene            complement(1798..2784)
                     /locus_tag="AC16CX_01880"
     CDS             complement(1798..2784)
                     /locus_tag="AC16CX_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011289.1"
                     /translation="MPPTPAATRKRSIALLAGCLALSCLHAAAQGYPSKPVRVIVPFP
                     PGAGADITTRLFAPRLSEALGQPVVVENRAGAAGNIGAEVVARAAPDGHTLLTAPASI
                     AIGQTLYKKLPFDMVRDFQPAAMLASVPFVLVVHPSLPVKNVKDLIALAKSRPGQINY
                     ASTGNGSSPHLTTEMLKMQARIDVVHVPYKGTPLAVTDLLTGQVSFMFANALSVLPHV
                     NAGRLRALGITSAKRGAATPRLPTLAEAGLPGFESGTWFAVLAPAGTPRDAVNRLSAE
                     VAKLTRHPDLREKLAAQGAEALTMTPEQTAAHIKSEIAKWGKVVLASGAKVE"
     gene            2983..3453
                     /gene="purE"
                     /locus_tag="AC16CX_01885"
     CDS             2983..3453
                     /gene="purE"
                     /locus_tag="AC16CX_01885"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3011290.1"
                     /translation="MGSQSDWEIMQHAARSLADFSVPYEARALSAHRTPDLLVEWVEG
                     LVKLGVKCFIAGAGGAAHLAGVVAARTTLPVLAVPMPSEHLQGLDSLLSMVQMPKGIP
                     VATFAIGDAGAANAGLFAVAMLALGDERLAKKLADFRKKQAEAGKAVKLPGLSG"
     gene            3524..4414
                     /locus_tag="AC16CX_01890"
     CDS             3524..4414
                     /locus_tag="AC16CX_01890"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR3011291.1"
                     /translation="MTTNHSTLFESNLKSLKFLHRGKVRDLYAVGNDRLLVIQTDRLS
                     AFDVIMPDPIPEKGRVLTGMSFFWFKKLGHVIANHLTGVAPESVVAPDERGQVAGRAM
                     VVRKLKPLMIEAVVRGYIIGSGWKDYQKTGMVCGIKLPAGLREAEQLPEVIFTPATKA
                     PAGSHDENISFAEAERIVGAGLAAKVRAVSIRLYSEAAAYAKNRGIIIADTKFEFGTD
                     DEGGLVLIDEILTPDSSRFWAAAEYRTGISPPSFDKQIVRNWLETQPWNKKAPAPKLP
                     PEVLRKTSEKYQEVLRLLTQ"
     gene            4417..5529
                     /locus_tag="AC16CX_01895"
     CDS             4417..5529
                     /locus_tag="AC16CX_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA];
                     GO_process: GO:0070588 - calcium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:cation antiporter"
                     /protein_id="HIR3011292.1"
                     /translation="MLSPSTVRREWQLLFSAATTALFLVFGEFWLADLSHPAWFTVVL
                     CWLFAAILLSAFAVVRHAELLAAKLGEPLGTLILTLAVTGIEVAMIAALMFTGKGNPA
                     LARDAMFAVVMIIMNGMVGLSLVLGGLRHHEQTYNLQGANAFLAVIVPLAVLCMVLPN
                     FTTAAPGPTLSPAQAVFFIVISVGLYGVFLAIQNLRHRAYFMAPESPDVADVQPAAGP
                     GFRSVPYHASLLVTYMLPLVILAKQLAVPIDLGITMLRAPVALGGFLVAVVVLSPESM
                     SAVRAALANELQRSVNILLGSVLATISLTIPAVLVIGFVADRTIVLGLDAANMVLLVL
                     TLGVSTLTFASPRTNVLLGAVHMLLFLAYLMLMFEQ"
     gene            5534..6514
                     /locus_tag="AC16CX_01900"
     CDS             5534..6514
                     /locus_tag="AC16CX_01900"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3011293.1"
                     /translation="MATERDIEQAVGMLRAGGLVAFPTETVYGLGADASNPAAVRKIF
                     EVKGRPATHPLIVHLADAVQLANWARQVPDGARRLARKFWPGPLTIILKRASNVSDVV
                     TGGQDTVALRVPAHPVAQQLLARFGGGIAAPSANRHGRVSATTADHVRREFGNAVACV
                     LDGGEAKVGIESTIIDLSGASAVLLRPGWITAGEIEEALGASLAAAAAGAPQAPGTLA
                     SHYAPQTPLMLMEGDLAIEFAASLTRQGKRVAVLALSTLQPLMPGVKWIAAPRDAAGY
                     AHALYASLRRLDEAGCDTIIVEKPPQAPEWAAINDRLARAAAGSSVPDDT"
     gene            complement(6565..>7909)
                     /locus_tag="AC16CX_01905"
     CDS             complement(6565..>7909)
                     /locus_tag="AC16CX_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3011294.1"
                     /translation="FNVPPVAALIMLAGIYYGAQYGGSTTAILVNLPGETSAVVTCID
                     GYQMARQGRAGPALAIAAIGSFFAGTVCTLIIALFGPPLAEMALKFGAPEYFSLMLMG
                     LVMAAVLAHGDMIKSLAMVAMGLLLGIVGTDVNSGMARYSFGVPELTDGIGFIVIAVG
                     VFAVGEIVSNLGETEERKVFTSNVTGLMPTKEDLKQSLGPIIRGTAIGAFFGVLPGTG
                     PAIASFSSYMVEKKMAKDPSRFGNGAIEGVAGPESANNADAQCKFIPTLTLGIPASAV
                     MALMLGALTIQGIAPGPQVMTQKPDLFWGLVASMWIGNLMLVVLNLPMVGLWVMLLKV
                     PYRLLFPAIMAFSCIGIYSVNNSAFELYLTALFGVIGFMWMRLECPPAPMLLGFVLGP
                     LMEENLRRALLISRGDPTVFVTRPISLGFMIATVLVIVIMVAPAVKARREQITGG"
BASE COUNT         1296 a         2625 c         2680 g         1308 t
ORIGIN      
        1 ggaacgccgt gccgttgcgc ttctcccgct ccgccatcgc cagcgccgcg ggcacgatgg
       61 cgcagccggg gtggcagagg gagggcgcgt gcgaatcgtc ggtctcgtcg gcgtgaccga
      121 gcatgccgtt gacgagcgcc gcatgctgcg cggaagtgac gaggcgcgag ccgatgacgc
      181 aggcttccct tgcgccgccg agcgtcttca cataggcgat cgccttcttc ccgggcagca
      241 gccgcgagcc cgagaccatt gcggcgatcg tgtcgagcag gtgatgcttg gtcttctcga
      301 cgacctcctg cggcagcggc cgcttcagtg cgctggcgat atactccgcg acctcccgca
      361 tcaacggcga gatttccgct tcggttttct tccttccttt cacgttttca tctccttgcc
      421 gttgatcttt actcgacact cgacactcga cactcgacac tcgtcactgc ggcaccgcga
      481 cgcgggcaag cgccccggcg tcctccagcg cggtgatccg cctgcataga tggatggtct
      541 cctcgacctg agcgggcgga agcactttct ccgcgagccc cttgaatttc gcctcgaggt
      601 cggcgtcgct catcgggcgc gcgagcgtcc cgagcgcgtg ctcgacgtga cggtcgaacg
      661 tgcgtccgtc ccgggtgcgg atggtaacac gcgcctccgt cctgccgatg gcggggtcca
      721 cccgagtgtt caccctgcct ctcaggtcga caacacgcgg gtcgcgcacg caggcgtcgc
      781 tgaactgcgc ctcgcccgcc gcgccgtgga cgatggccgc ggagacggca tggtagacgc
      841 tgaacttgcc ctcgagcccg gtcagcggcg cggtattgcc ggtgagctcg agcacgagcg
      901 ggctcactgt gagctcgacc gcctcgatgg cttccggcgc cagccggtgc tcgcggcaga
      961 tttcgatgca accgtcaatc gccgcgtgca ccaccagtcc gcaggcgtag ggtttgtaca
     1021 tgttggacag gatctcgtag cgctcgccca gcccctcggt gattacggag gggtcgaact
     1081 tgggggaact gacacgggca aaaccgcgcg gcgcctcgat cgcgcgctcc gagctggtga
     1141 atccccgggc ggccagcaat gcggcggtga ggccgttttg tgccgcgcgt ccggggtgaa
     1201 ggctcttgca catgctgccg aacatctcgc gcaggcccgc cgcctgcgtc gccgcgatgc
     1261 ccagcgccca gcccatctgc cgctcatcga gcgcaagcag cttgcccgcg gccgccgcgg
     1321 cgccgaacac gccggccgtg ccggtaatat gccagcccgc gttgtaatgc tcggggaata
     1381 ccgagagccc gacacgctct tcgacctcga cgcccagcac gaacgcgtgc aagagcttcg
     1441 cgccgttgat cctgcgccat tccgcgagcg ccagcacggc cggcagcacc ggcgcgctgg
     1501 ggtggatcgc acgcgcgtgg gtatcgtcga agtcgagcac gtgcgagctg atgccgttga
     1561 tcagggccgc gtgcaggatg tcgaggcgct cgccgcggcc gagcaccgcc gcctgcggcg
     1621 ggccgcagaa aggctgcagc gccgcgagcg cgcgctccac ggtctcgtga cggcaaccgc
     1681 cgaccgcgca gccgacccag ttgagcagcg cgcgcgcggc ctcgtgacgc acgcgttccg
     1741 ggatatcctc ccagcgcgaa gtcaccaggt agcgggcgag gatgcgggtg acatccatca
     1801 ttcgaccttg gcgccggagg ccagcaccac cttgccccac ttggcgatct cgctcttgat
     1861 gtgcgccgca gtctgctccg gcgtcatggt gagcgcttcc gcgccttgcg ccgcaagctt
     1921 ttcgcggagg tcgggatgcc gtgtgagctt cgcgacctcg gcgctgagcc ggttcaccgc
     1981 atcgcgcggc gtgcccgcgg gggcgagtac cgcgaaccag gtgccggact cgaagcccgg
     2041 caggccggct tcggccagcg tcggaagccg gggcgtggcg gcgccgcgct tcgcgctggt
     2101 gataccgagc gcgcgcagcc gcccggcatt cacgtgcggc agcaccgaca aggcgttggc
     2161 gaacatgaac gacacctgcc ccgtgagcag atccgtgaca gcgagcggcg tacccttgta
     2221 gggaacgtgc acgacgtcga tgcgcgcctg catcttgagc atctccgtgg tgaggtgggg
     2281 cgagctgccg ttgccggtgg aggcgtaatt gatctggccg ggccgcgatt ttgcgagcgc
     2341 gatgaggtcc ttgacgttct tcaccggcag cgaggggtgc accacgagga cgaacggcac
     2401 cgaggcgagc atggcagccg gctggaaatc gcgaaccatg tcgaatggaa gcttcttgta
     2461 gagcgtctgc ccgattgcga tcgatgccgg ggcggtgagc agcgtgtggc cgtccggggc
     2521 ggcgcgcgca acgacctccg cgccgatatt gccggctgcg ccggcgcgat tctcgaccac
     2581 gaccggctgg ccgagcgcct ccgacagcct gggcgcgaac agacgcgtcg tgatgtcggc
     2641 tcccgcgccg ggcggaaacg ggacgatcac ccgcaccggc ttcgatggat agccttgggc
     2701 cgcggcatgc aggcaggaaa gcgcgaggca gccggcgagc agtgcaatcg agcgctttct
     2761 cgtggcagcg ggcgtgggcg gcaccgggaa tcctttcggg gaggacgcga agcggctggt
     2821 tgcgacgaaa gcccgcgacg cgtcctgttt gaggcggttc ctccgcgaaa atcttatcag
     2881 ccgcgggcgg cgggctcaac cggggacggc gacggtgcga tagaataacg cgctcgcaca
     2941 aaggggagcg cagccgtgag caaaccgctg gtcggcatcg tgatggggag tcaaagcgac
     3001 tgggagatca tgcagcacgc cgcgcgcagc cttgccgatt tcagcgtgcc gtatgaagca
     3061 cgggcgctgt cggcgcaccg cacgcccgac ctcctcgtcg agtgggtcga aggactggtg
     3121 aagctgggcg taaagtgttt catcgccggt gcgggcggcg cggcgcacct tgccggcgtg
     3181 gtggcggcca ggacgacgct cccggtactg gccgtgccga tgccctccga gcacctgcaa
     3241 gggctcgatt cactgctttc gatggtgcag atgcccaagg ggatcccggt cgccactttt
     3301 gccattggcg acgcgggggc cgccaacgcc gggctgttcg ccgtggcgat gctcgcgctc
     3361 ggggacgagc ggctggcgaa gaagctcgcg gacttccgca agaagcaggc cgaagccgga
     3421 aaggccgtga aacttcccgg cctttccggc tgatggaggg ggtggccgca ggctcaactc
     3481 acgaatcacg attcacgaat cacgcctttt ccctccaagc aagatgacga cgaatcattc
     3541 cactcttttc gaatcgaatc tgaagagcct gaagttcctg caccgcggca aggtgcgcga
     3601 tctctacgcg gtggggaatg accggctgct cgtgatccag accgaccggc tgtccgcgtt
     3661 cgatgtgatc atgcccgacc cgatcccgga aaaagggcgc gtgctgaccg ggatgtcatt
     3721 cttctggttc aagaagctcg gccacgtcat agcgaatcac ctgaccggtg tcgcgccgga
     3781 atcggtggtg gcgccggatg agcgcgggca ggtggcggga cgcgcgatgg tggtgcgcaa
     3841 gctcaagccg ctcatgatcg aggcggtggt gcgcggctac atcatcggct cgggctggaa
     3901 ggattaccag aaaaccggga tggtgtgcgg catcaagctg cccgcggggc tcagggaggc
     3961 ggaacagctg ccggaggtga tcttcacccc ggccaccaag gcgccggccg ggagccacga
     4021 cgagaatatc tcgttcgcgg aagcggagag gatagtcggc gccgggcttg cggcgaaggt
     4081 gcgcgcggtg tcgatacggc tctacagcga agccgccgca tacgcgaaaa acaggggcat
     4141 catcatcgcc gacaccaagt tcgagttcgg taccgacgat gaaggcgggc tggtgctcat
     4201 cgatgaaata ttgacccctg attcctctcg attctgggca gccgcggagt accgtaccgg
     4261 aatcagcccg ccttcatttg acaagcagat cgtgcgcaac tggctcgaga cccagccgtg
     4321 gaacaagaag gcccccgcgc cgaagctgcc gcccgaggtg ctccggaaga cctccgagaa
     4381 ataccaggaa gtcctgcggc tgctcacgca gtaggaatgc tttccccttc gacggtgcgc
     4441 cgggaatggc agctactctt cagcgccgcg acgacggcac tgttcctggt tttcggcgag
     4501 ttttggctgg cggacctctc ccacccagcc tggttcaccg tcgtgctgtg ctggctgttc
     4561 gccgccatcc tgctgtcggc cttcgcggtg gtgcgccacg cggaactcct ggccgcgaag
     4621 ctgggcgagc cgctcggcac gctgatcctg acgctcgccg tcactggcat cgaagtcgcc
     4681 atgatcgccg cactcatgtt cacggggaag ggaaatcccg cgctcgcgcg cgacgcgatg
     4741 ttcgcggtgg tcatgatcat catgaatgga atggtgggcc tgtcgctggt gctgggcggc
     4801 ttgcgccatc acgagcagac ctacaacctc cagggcgcaa atgcgttcct ggccgtcatt
     4861 gtgccgctgg cggtgctgtg catggtgctg cccaacttca ccacggcggc gccgggtccg
     4921 acgctgtccc cggcgcaggc cgtcttcttc atcgtcatat cggtcgggct ctacggggta
     4981 ttcctggcga tacagaacct gcgccaccgg gcctacttca tggcgcctga gtccccggat
     5041 gtggcggatg tgcagccagc agccggccct ggcttccgct cggtcccgta ccacgcatcg
     5101 ctgctcgtca cgtacatgct gcccctcgtc atcctggcca agcagctcgc ggtgccgatc
     5161 gacctcggca tcaccatgtt gcgcgcgcct gttgctttgg gaggattcct tgtcgcggtc
     5221 gtggtgctgt ccccggagtc catgagcgcg gtgcgcgcgg cgcttgcgaa cgagctccag
     5281 cgctcggtga acatactgct cggctcggtg ctcgcgacca tcagcttgac catcccggcg
     5341 gtgctcgtca tcggtttcgt ggccgaccgg actatcgtcc tcgggctcga tgccgcgaac
     5401 atggtactgc tggtgcttac gctgggagtg agcacgctga ccttcgccag ccctcgcacc
     5461 aacgtcctgc tcggcgcagt gcacatgctg ctgttcctgg cgtacctgat gctcatgttc
     5521 gagcagtgac gagatggcca ccgaacgcga tatcgagcag gcagtgggca tgctgcgtgc
     5581 cggagggctc gtcgcctttc ccacggagac ggtgtatggc cttggcgccg acgcctccaa
     5641 tccggcagcg gtccggaaaa tcttcgaggt caagggccgc ccggcgacgc acccgctgat
     5701 cgtgcacctc gcggacgcgg tgcagctcgc caactgggcg cggcaggtcc cggatggcgc
     5761 gcgccggctc gccagaaaat tctggccggg cccgctcacc attattctca aacgcgcctc
     5821 gaacgtgagc gacgtcgtga ccggcggcca ggacacggtg gcgctgcgcg tgccggctca
     5881 ccccgtggcg cagcaactgc tcgcgcgctt cggcggcggc atcgccgccc cctccgccaa
     5941 ccgccacggc cgcgtctccg ccaccaccgc cgatcacgtg cggcgcgagt tcggaaatgc
     6001 ggtcgcgtgc gtgctcgacg gcggcgaggc gaaggtcggc atcgagtcca ccatcatcga
     6061 tttgtccggc gcgagcgccg tgctgttgcg ccccggctgg atcaccgcgg gtgagatcga
     6121 ggaggcgttg ggagcgtcgc ttgccgcagc ggcggcgggc gcgccgcagg cgccgggcac
     6181 gctcgcgtcc cactacgcgc cgcagacgcc attgatgctc atggaaggcg atctggcgat
     6241 cgagtttgcc gcgagcctga cccgccaggg caagcgggtc gcggtgctcg cgctctcgac
     6301 gctgcagccg ttgatgccag gagtgaaatg gatcgccgcg ccgcgggatg cggcgggcta
     6361 cgcgcacgcg ctgtacgcca gcctgcgccg tctcgacgag gcggggtgcg acacgatcat
     6421 cgtggagaag ccgccgcagg cgcccgaatg ggcggcgatc aacgaccggc tcgcccgcgc
     6481 cgccgccgga tcatccgtgc cggacgacac ctgaagaaaa cggcgcctct cgggcgccgt
     6541 tttcgctgag cgtgatccgc ttcctcagcc gccggtgatc tgctcgcgcc ttgccttgac
     6601 cgcgggcgcg accatgatga cgatgaccag caccgtcgcg atcatgaaac cgaggctgat
     6661 cggacgggtg acgaacaccg tgggatcgcc gcgcgagatc aggagcgccc ggcgcaggtt
     6721 ctcctccatc agcggcccca gcacgaagcc gagcagcatc ggcgccggcg ggcactccag
     6781 cctcatccac atgaaaccga tgacgccgaa gagcgcggtg aggtagagct cgaacgcgct
     6841 gttgttcacg ctgtagatgc ctatgcagga gaacgccatg atggcgggga acagcagccg
     6901 gtacggcacc ttcagcagca tgacccacag gccgaccatc ggcaggttga ggaccaccag
     6961 catgaggttc ccgatccaca tgctcgcaac gagaccccag aacaggtccg gcttctgggt
     7021 catcacctgc ggccccggcg cgatgccctg gatggtgagc gcgcccagca tgagcgccat
     7081 caccgcgctc gccgggatgc cgagcgtcaa ggtcggaatg aacttgcact gggcgtcggc
     7141 gttgttcgcg gactcggggc ccgccacgcc ctcgatcgcg ccattgccga agcgcgaggg
     7201 gtccttcgcc atcttcttct ccaccatgta ggaggagaac gaggcgatcg cgggcccggt
     7261 gccgggcagc acgccaaaga acgcgccgat ggcggtgccc cggatgatcg gccccaatga
     7321 ttgtttcagg tcctccttgg tgggcatgag gcccgtcacg ttggaggtga acaccttgcg
     7381 ctcctcggtc tctccgaggt tgctcacgat ttcccccacg gcgaagaccc ccacggcgat
     7441 gacgatgaag ccgatgccgt cagtcagctc gggaacaccg aagctgtagc gcgccatgcc
     7501 ggaattgaca tcggtgccca cgatgccgag cagcagtccc atggccacca tcgccagcga
     7561 cttgatcatg tcgccgtgcg ccagcaccgc cgccatgacg aggcccatca acatcagcga
     7621 gaagtactcg ggcgcgccga acttgagggc catctcggcg agcggcgggc cgaacagcgc
     7681 gatgatcagc gtgcaaaccg tgccggcgaa gaacgagccg atggccgcga ttgcgagcgc
     7741 cgggccggca cgcccctggc gcgccatttg gtagccgtct atgcaagtga ccaccgcaga
     7801 ggtctcgccc gggaggttca ccaggatggc ggtggtggag ccgccgtact gcgcaccgta
     7861 gtaaatcccc gccagcatga tgagcgccgc gaccggtggc acgttgaag
//
LOCUS       DBNGWZ010000069         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1643084, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000069 DBNGWZ010000000
VERSION     DBNGWZ010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1643084"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /gene="ispH"
                     /locus_tag="AC16CX_01910"
                     /gene_synonym="lytB"
     CDS             complement(<1..136)
                     /gene="ispH"
                     /locus_tag="AC16CX_01910"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811701.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3011295.1"
                     /translation="MKVLLANPRGFCAGVDRAIEIVERALALHGAPIYVRHEVVHNKF
                     V"
     gene            complement(209..706)
                     /gene="lspA"
                     /locus_tag="AC16CX_01915"
     CDS             complement(209..706)
                     /gene="lspA"
                     /locus_tag="AC16CX_01915"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3011296.1"
                     /translation="MAPMTKWLELAALIVAADQLAKYAAVQNLAVGQAVAVTSFFNLA
                     LVYNAGAAFSFLSDAAGWQRGLFIAIALAASAWIVWLLHRYPRQRLFAFALTLVLAGA
                     VGNVIDRITVGAVVDFLDFHAFGHHWPAFNVADSAITCGAVLLVWDALRPKKSENLDA
                     KSQRR"
     gene            complement(824..>2208)
                     /locus_tag="AC16CX_01920"
     CDS             complement(824..>2208)
                     /locus_tag="AC16CX_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3011297.1"
                     /translation="HPRALELLEQVAKRVEHGGIESWQSLEAEELLGDDAARYEKVKD
                     TLDVWFDSGSTHYTVMRGSHRAETGFPADLYLEGSDQHRGWFHSSLLVASMMDGVPPY
                     RGLLTHGFVVDGEGYKMSKSKGNVIAPQEVADELGADILRLWVAATDYSGELSISREI
                     LKRVVESYRRIRNTLRFLLANLADFDLARDVQPVDEWLEIDRYAWVMTHDLQAALTPS
                     DPGVRNPAAPGHYGNYEFHLVAQKLQTFCSEDLGGFYLDILKDRLYTAPANSRARRSA
                     QNALYHITHALTRLLAPILSFTAQEAWEVLVGGEASVFEQTWYEFRLPRDAGELRSRW
                     TKLRALRSDVLKQLEELRIEGKIGSSLAGEVDLYANGREREFLGSFADDLRFVFITSR
                     ADLAAEAPSGAKSTALSDVSIKVTPSPHKKCDRCWHYRADVGKDPAHPGLCGRCLANL
                     FGPGEPRSHA"
BASE COUNT          429 a          714 c          703 g          362 t
ORIGIN      
        1 ccacgaattt gttgtgcacc acctcgtggc gcacgtagat cggcgcaccg tgcaacgcaa
       61 gcgcgcgctc gacgatttcg atcgcgcggt cgacgccggc gcagaatccg cggggattgg
      121 cgagcagaac cttcatgacg gaaattatac aaccagacgc aaaggcgcga aggcgcaaag
      181 aaagacgacc atcaataaat ccataccctt agcgtctttg cgactttgcg tcgaggtttt
      241 cgcttttctt cgggcgcaga gcgtcccaca ccagtagcac cgcgccacat gtgatggcgg
      301 aatccgcgac gttgaacgcc ggccagtgat ggccgaacgc gtgaaagtcg agaaaatcga
      361 ccaccgcacc gaccgtgatg cggtcgatga cgttgccgac cgcgccggcg agcaccagcg
      421 taagcgcgaa cgcgaacagg cgctggcgcg ggtagcggtg cagcagccag acgatccacg
      481 ccgaagcggc gagcgcgatc gcgatgaaca gcccgcgctg ccagccggct gcatccgaca
      541 ggaagctgaa ggcggcgccg gcgttataga ccagcgccag gttgaagaac gaggtcaccg
      601 cgactgcctg ccccacggca aggttttgca cggccgcgta cttggcaagc tgatccgccg
      661 ccacgatcag cgccgcgagc tcgagccact tcgtcatggg agccatttca aaagtgcgga
      721 aatggaaaat gggaaatggg aaatgggaag atcccgggcg cgccggccag acccgctact
      781 cgctccacca tttcctattt cctattcccc attcccggaa acatcacgcg tgcgagcgcg
      841 gttccccggg accgaacaaa ttcgccaggc agcgtccgca tagcccgggg tgagccggat
      901 ccttgcccac atccgcacga tagtgccagc agcggtcgca cttcttatga gggctcggcg
      961 tcaccttgat actgacgtca gacaatgccg tggacttcgc gccagaaggt gcctctgccg
     1021 ccagatcggc ccgggaggtg atgaacacga acctcaagtc atcggcgaac gaccctagaa
     1081 actctctttc ccggccattg gcgtacaggt cgacctctcc ggcgagcgag gagccgatct
     1141 ttccctcgat gcgcagctcc tcgagctgct tgagcacgtc ggagcggagc gcccgcagct
     1201 tggtccaacg gctgcgcagc tccccggcgt cacgcggcaa ccggaattcg taccaggtct
     1261 gttcgaacac gctcgcttcg ccccccacca gcacctccca cgcttcctgc gcggtgaacg
     1321 agaggatggg cgcgagcagc cgggtgagcg catgcgtgat gtggtagagc gcgttctgcg
     1381 ccgagcgccg ggcgcgcgag ttggcgggcg cggtgtagag gcggtccttg aggatgtcga
     1441 gatagaaacc gccgaggtct tcggagcaga aggtctgcag cttctgcgcc accaggtgga
     1501 actcgtagtt gccgtaatgg cccggcgccg cgggatttct gacgcccggg tcggagggcg
     1561 tgagagccgc ctgcaggtca tgggtcatca cccacgcgta acggtcgatc tccagccact
     1621 cgtccaccgg ctggacgtcg cgcgccaggt cgaaatcggc gaggttcgcg agcaggaaac
     1681 gcagcgtgtt gcggatgcgc cggtacgatt cgaccacgcg cttgaggatt tcacgcgaga
     1741 tcgagagctc gccggaataa tcggtggcgg cgacccacag gcgcaggatg tcggcgccga
     1801 gctcgtccgc cacttcctgc ggcgcgatca cgttgccctt cgacttgctc atcttgtagc
     1861 cttccccgtc caccacgaag ccgtgggtga gcagtccccg gtacggcggc acgccgtcca
     1921 tcatggaggc gacaagcagc gaggaatgga accaaccgcg gtgctggtcg gagccctcaa
     1981 ggtagaggtc ggcggggaac ccggtttcgg cccggtgcga gccgcgcatc accgtgtaat
     2041 gcgtcgagcc ggaatcgaac cagacatcca acgtgtcctt caccttctcg tagcgcgccg
     2101 cgtcgtcgcc gagcagctcc tccgcctcga gcgattgcca gctttcgatg ccgccatgct
     2161 cgacgcgctt ggcgacctgc tcgagcagct cgagcgcgcg cgggtgca
//
LOCUS       DBNGWZ010000070         2987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2146639, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000070 DBNGWZ010000000
VERSION     DBNGWZ010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2146639"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..883)
                     /locus_tag="AC16CX_01925"
     CDS             complement(95..883)
                     /locus_tag="AC16CX_01925"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3011298.1"
                     /translation="MKFGRFEQNGRVFYGTVEGDQITELEGSIFDVYKTTSRKHALSS
                     LKTLVPRMPSNFYCAGLNYAAHIEWANKRTGGNRKVPEKADIGYRSANALIATGEAIV
                     IPADSPGPVQFEGELVAVIGRKARNLSEKDALSCVLGYTLGNDVSERTWQKQDRTLWR
                     AKNCDSFKPMGPFIVTGLDPMKLAISIKVNGSTASEYGTDKMLFSAQHYISQISKYMT
                     LWPGDVVWLGTDGATEPDLKDGDTVEITQKDIGTLRNLVVRAKA"
     gene            complement(1046..1432)
                     /locus_tag="AC16CX_01930"
     CDS             complement(1046..1432)
                     /locus_tag="AC16CX_01930"
                     /inference="COORDINATES: protein motif:HMM:NF020376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HIR3011299.1"
                     /translation="MSPERCFWTALAVAAVSTAAPAPAAEAVIVVQRSPLAGFRHYDG
                     REVWRDMKVGDRLELVRESDNSYDANAIRIEWRGLKLGYVPRRDNAAVARQLDRGAAL
                     EARVTTLRENRNRSVRIEFEVVAPLQ"
     gene            complement(1438..1851)
                     /gene="hemJ"
                     /locus_tag="AC16CX_01935"
     CDS             complement(1438..1851)
                     /gene="hemJ"
                     /locus_tag="AC16CX_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR3011300.1"
                     /translation="MLWIKAFHIIFLVAWFAGLFYLPRLFVYHAMTEDAAGRERFKVM
                     ERKLYYGIMAPGAVLAIVFGLWLWLGYGITGGWLHAKLALVALLIGYHLWCGKLMGNF
                     KHDRNTRSHVWYRWFNEAPTVILIVAVILTVVKPF"
     gene            complement(1988..2773)
                     /locus_tag="AC16CX_01940"
     CDS             complement(1988..2773)
                     /locus_tag="AC16CX_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011301.1"
                     /translation="MNAKTLAIPAFSLRHIHVWRRNSLVWRKLAIPSMLGNLADPMLY
                     MLGLGYGLGTMLADVGGMPYIAFLAAGTVCSSAMMSASFEAMYSGFSRMHVQKTWDAI
                     MNAPVTLDDVVLGEAVWAASKSFLSGMAVLLVAWVLGLVHSASALWAIPVVFLTGLTF
                     ASLGLIMTALSPSYDFFMYYFTLVITPMMLLCGVFFPVAQLPAPFQAVTQVLPLTHAV
                     DLVRPLMNGVMPAEIPLHLSVLAAYAVAGFYIALVLTRRRLLT"
BASE COUNT          587 a          924 c          942 g          534 t
ORIGIN      
        1 tcgggagatc gggagatcgg gagataggaa aaccgggggt cgtgtgtagg tttcgctctc
       61 acgccctcac gctctcccgc tctcacggat catgtcaagc tttggcgcgc acgaccagat
      121 ttctgagcgt gccaatatcc ttctgagtga tttcgaccgt gtcgccgtcc ttgagatccg
      181 gttcggttgc gccgtcggtg ccgagccaca ccacgtcgcc gggccagagc gtcatgtact
      241 tcgagatctg ggagatgtaa tgctgcgccg agaacagcat cttgtcggtg ccgtactcgg
      301 acgccgtgct cccattcacc ttgatcgaga tggcgagctt catcgggtcc agcccggtga
      361 cgatgaacgg ccccatcggc ttgaagctgt cgcagttttt cgcccgccac agagtgcggt
      421 cctgcttctg ccaggtgcgc tcgctgacgt cgttgccgag cgtgtagccg aggacgcacg
      481 acagcgcgtc cttctcggag aggttgcgcg ccttcctgcc gatcacggcg acgagctcgc
      541 cctcgaactg gaccggcccc ggcgagtcgg cgggaatcac gatcgcctcg ccggtggcaa
      601 tcagcgcatt ggccgagcgg tagccgatgt ccgccttctc cggaaccttg cggttgccgc
      661 cggtgcgctt gttggcccac tcgatgtgcg cggcgtaatt gaggcccgcg cagtagaagt
      721 tggacggcat gcgcggcacg agcgttttga gcgatgagag cgcgtgcttc ctggaggtgg
      781 tcttgtaaac gtcgaagatc gagccttcca gctcggttat ctggtcgccc tcgacggtgc
      841 cgtagaaaac acgcccattc tgctcgaaac gcccgaactt cattggaccc ccccgaatct
      901 ggtcagcccg tctgaataaa caagattgat cacaagaatg gcgccgagga tagcagagct
      961 ttcagcaatt agcattcagc tgtcagcttt aaacggatgt cgctgggggc ctaaagctga
     1021 tcgctgacgg ctgaccgccc cgcggtcact gcagcggggc tacgacctcg aactcgatgc
     1081 gcacgctgcg gttgcggttt tcccgcaacg tggtcacgcg ggcttcgagc gccgcgccgc
     1141 ggtccagctg gcgtgcgacc gcggcgttgt cgcggcgcgg cacatagccc agtttgaggc
     1201 cccgccactc gatccggatc gcgttcgcat catacgagtt atccgattca cgcaccagtt
     1261 cgaggcggtc gcccaccttc atgtcccgcc acacctccct gccgtcgtaa tgacggaacc
     1321 cggcgagcgg ggagcgctgc acgacgatca ccgcttccgc ggctggggcg ggagcagcgg
     1381 tcgataccgc ggcaacggcg agcgccgtcc agaagcatct ttcaggggac attcccctta
     1441 gaacggttta acgaccgtga ggatgacagc cacgatcaga atgacggtcg gcgcctcgtt
     1501 gaaccagcgg taccacacgt ggctgcgggt gttgcggtcg tgcttgaaat tgcccatcag
     1561 cttgccgcac cagaggtggt agccgatcag cagggcgacg agcgcgagct tcgcatgcag
     1621 ccaccccccg gtgatgccgt agcccagcca cagccacagg ccgaaaacga tggcgagcac
     1681 cgcccccggc gccatgatgc cgtagtagag cttgcgttcc atcaccttga agcgctcgcg
     1741 gccagccgcg tcctccgtca tcgcgtgata gacgaacagc cgcggcagat agaacagtcc
     1801 cgcgaaccat gccaccagga agatgatgtg aaaggccttg atccagagca ttgtgacgag
     1861 ctgtgatgag tgatgggtga tgagtgacgg gtgtcctgat aattgccgtg gtccgcgtga
     1921 tgaacggcag ccattctgtc cgttcgatca cgcgggcgtc aaccggcgct ccacgtccgg
     1981 gttgacgcta ggttaggagg cggcggcgcg tcagcaccag cgcgatatag aagcccgcca
     2041 cggcgtaggc ggcgaggacg gaaagatgca gggggatttc cgccggcatt acgccgttca
     2101 tcaacggccg caccagatcc acggcatggg tgaggggcag gacctgcgtc accgcttgga
     2161 acggcgcggg aagctgggcg accgggaaga acacgccgca cagcagcatc atgggcgtga
     2221 tcaccagcgt gaagtagtac atgaagaagt cgtagctggg ggagagcgcc gtcatgatga
     2281 gacccaggct ggcgaaggtc agccccgtca ggaacacgac gggaatcgcc cacaacgcgc
     2341 tagcagagtg cacgagcccc aacacccacg ccaccagcag caccgccatg cccgaaagaa
     2401 agctcttgct tgccgcccag actgcctcgc ccagcaccac gtcatcgagc gtcaccggcg
     2461 cgttcatgat tgcgtcccac gtcttctgca cgtgcatgcg cgagaagccc gagtacatcg
     2521 cctcgaacga ggcgctcatc atggcgctcg agcagaccgt gccggcggcc aggaacgcga
     2581 tatagggcat gccgcccaca tcggcgagca tcgtgccgag tccgtagccc aaccccagca
     2641 tgtagagcat gggatcggcg aggttgccga gcatggaggg aatcgcgagc ttgcgccaca
     2701 cgagggagtt gcggcgccac acgtggatgt ggcgcaggct gaatgcggga attgccaggg
     2761 tcttcgcgtt catgtcaggt cctcacttct gccattcaaa aagtgactcg tttctcgtca
     2821 ctggaagaag gctgcgaatc ggacacgacg agtgacgagc aacgaaaaac gaatcacctt
     2881 ttgaatgaca gaagtgagga cctgacatga acgcgaagac actggccatt ccttcgttca
     2941 gcctgcgcca cctgcacgtg tggcgccgca attcgctggt ctggcgc
//
LOCUS       DBNGWZ010000071         5796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1391203, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000071 DBNGWZ010000000
VERSION     DBNGWZ010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1391203"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1282)
                     /locus_tag="AC16CX_01945"
     CDS             complement(86..1282)
                     /locus_tag="AC16CX_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3011302.1"
                     /translation="MTSLHPAPAWFVVLVTTAAQMVVAMSNIVLSTIAPKVAESLGVD
                     PVLIGYQVGLTFAAAAVASVYGGVVAMRLGSAFTTQLSMVSSVIGLTLFALPHVGFIA
                     LGSLAVGIGSGLASPSASYLLANHTSPERRNLMFSIKQTGVPVGGVIVALTAPLLAVT
                     VGWRWALAMIAAMAVATLIVTERARAAWDGERRSAGGVPSAPFGGVPLVWREKALRWI
                     ALAAVLFSSVQRVLLSFTVVYLVAEGGYGLVEAGVMLSVAQVGGSVFRIPWGWLADRL
                     RSGLAVLTIICVIMIAASVLLVLLETSWPKPLVTLLFFVLGASCVGWNGIVHAECARL
                     SPPGMISLVAGGTSFFVFGGVMLGPTVFAAAYGVIGSYSATFALMVVLGVAALGVLRL
                     AHRQQR"
     gene            1536..2261
                     /locus_tag="AC16CX_01950"
     CDS             1536..2261
                     /locus_tag="AC16CX_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3011303.1"
                     /translation="MAHTLAGRASTDVRAMPLLSDRAYERIRHDIITCAIAPGTEISE
                     AQLGAHYKLGKAPVRMALNRLAHDGLVRAIPRRGYRVMEVTLKDIHDVFELRLMLEPA
                     AARMAAGRVDAQRLRALDEVCRAGYQRGDARSTSRFLEANKAFHVEIARAAGNVRLAG
                     AIEQLHDEMTRLLHLGLGLRNRSHEMQREHRSLVKALERGDGATAERICREQIEAARS
                     MVLSAILTSSSVMNLALGVEGPR"
     gene            2258..2761
                     /locus_tag="AC16CX_01955"
     CDS             2258..2761
                     /locus_tag="AC16CX_01955"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF017778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HIR3011304.1"
                     /translation="MSALPQLRHLADRIGAAVGLAPDAMASEIKAALRDATAGANWLP
                     SERRRASHENYARHLLYGDPDGRFSILAIVWDHGQMSPIHGHHCWCAVGVYQGVLTET
                     RYREGAGGPPVPVNSTRRAAGTLSFDPAASAIHRIANESGVLAVSLHVYGIAKDGIST
                     GVNRIYA"
     gene            2869..3876
                     /locus_tag="AC16CX_01960"
     CDS             2869..3876
                     /locus_tag="AC16CX_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3011305.1"
                     /translation="MKAVVLEKHGGLEELKFVKDFPDPKTADGHVVIRVRATSFNYHD
                     VFTVRGMPGIKVPLPMIIGLDMAGEILEVGPGVDPRWKKGDRVLVNPLNRQKGLMGEM
                     MHGGLAEKCLVAEHQLIRMPEGVTFEEAASLPVAYGTAHRMLITHKTVKSGDRVIVLG
                     ASGGVGTGCVLLAKMLGAEVIACASSEDKIKRLKELGADHVINYKETDFSKWAVEKYG
                     KPQRRTYEGGVDVVINFTGGDTWAPTLRCVKRGGKILVCGATAGYDPKEDLRYIWSFE
                     LQVIGSNSFYDDNLQALMGLIQEGRMKPVIDKVLPLERAAEGLRLIESREVFGKVVVT
                     P"
     gene            4000..5541
                     /locus_tag="AC16CX_01965"
     CDS             4000..5541
                     /locus_tag="AC16CX_01965"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3011306.1"
                     /translation="MPIPCPEPRGYPGNLGLLFAEHAHSERPAIIELREPKRPRPVSF
                     RELNAGCNAVARGLVKAGLKPGDRIGIVSLNRVEFVMTLLGAMRAGAVPVPVNIKLPA
                     DAVAYILNDAGARMTFAERDLKRLVPAALRVVEFGGAFDSFLDPGPFEAFEPAPDSIA
                     IQPYTSGSTGQPKGVLLTHYGQNWSRRILAYTRGTTEKDVILVAAPLYHKNALNAIKQ
                     GLTAGATLPLLPQFDVERYIEAIERHRCTVISGVPTMMAMVLSRKDLLAQIDPSSVRT
                     IMMGSAPSSPQLVKELRLHFLNAEPLVVYGVTEGGPVPLGPHPEGKPRPPGSIGVPYP
                     GTEAKLVGGSGPDEGELVLRNPGVLLGYHNLPEETAKRLREGWYYTGDICRRDADGFY
                     YFVGRTDDMFVSGGENIYPGEVEALLARHPAVHQALVMPFDHELKGQVPYAFIVRRAG
                     AAVTEDEIKRFALANGPAYQHPRRVFFLDQFPLAGTNKIDKQKLRQRVADGTLERPPA
                     AKGAL"
     gene            5541..>5796
                     /locus_tag="AC16CX_01970"
     CDS             5541..>5796
                     /locus_tag="AC16CX_01970"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3011307.1"
                     /translation="MTDQPLTAQQLREIMTSRPFHQWLGLDVLKSDETGIEIKAAWRE
                     EWVVNPERRYTHGGILAALVDVAADYALCAKIGRPVPTIDM"
BASE COUNT         1085 a         1922 c         1871 g          918 t
ORIGIN      
        1 gccgctctcc acatcggttg atcgtccctt cgcccgtcga gcggcgaccg ggcagtcgcg
       61 ccaacggttg cctgccgatc acgggtcacc gctgttgccg gtgggcgagc cgcaacaccc
      121 ccagcgccgc cacgcccagg acgaccatca gcgcgaacgt cgcgctgtag gacccgatca
      181 ctccgtacgc cgccgcgaac accgtcgggc ccagcatcac gccgccgaat acgaagaacg
      241 acgtgccgcc ggcgaccagg ctgatcatgc cgggcgggct caagcgggcg cattcggcgt
      301 gaacgatgcc gttccagccc acacaactcg cacccaacac gaaaaacagc agcgtcacca
      361 gcggtttcgg ccagctcgtc tcgagcaaga caagcaggac cgaggcagcg atcatgatca
      421 cgcagatgat ggtgagcacg gcgaggccgc tgcgcaggcg gtcggccagc catccccacg
      481 ggatccggaa aaccgagccg ccgacctggg ccacggacag catgaccccc gcctccacca
      541 gcccgtagcc gccctcagcg acgaggtaga cgacggtgaa gctcaacagg acgcgctgga
      601 cgctggagaa cagcaccgcg gcgagagcga tccagcgcag cgccttctcc cgccacacga
      661 gcggcacgcc gccaaacggc gcgctgggca ccccgcccgc ggacctgcgc tcgccgtccc
      721 aggcggcacg ggcgcgctct gtgacgatga gtgtcgcgac ggccatcgcg gcgatcatcg
      781 cgagcgccca gcgccagccg accgtcaccg cgagcaacgg ggccgtcaac gccacgatca
      841 cgccgcccac cggcaccccc gtctgcttga tcgagaacat caggttgcgg cgctccggcg
      901 acgtgtggtt ggccagcagg taggacgccg agggactggc gagccctgat ccgatgccga
      961 cagccagcga tcccaacgca atgaagccga cgtggggcaa cgcgaacaac gtcaagccga
     1021 tcacgctgga caccatgctc aactgcgtcg tgaacgcgga acccaggcgc atcgcgacga
     1081 cgccgccata gaccgaggcc accgcggcag cggcaaacgt caagcccacc tggtagccga
     1141 tcaacacggg atcgacgccg agcgattcgg ccaccttggg cgcgatggtc gagagcacga
     1201 tgttcgacat cgccaccacc atctgcgcgg cggtggtcac cagcacgacg aaccatgccg
     1261 gggcgggatg caaggatgtc atgggataag cgttcggcgg tcgataccgg ttccgcgcgt
     1321 tgagcggcat tcgtggaccg gtgttaccac cgtaccacgg ttgcccgctc gaacgcgcgg
     1381 agttaatccg gcgcccagcg tccgggatta acgaatggta gcaattaaga gggatgcttc
     1441 ccggtttcgt ccatactgtg gctgcccgcc gcgcttttga catgtcacga tcgcgttcat
     1501 agaatttcaa ctcttttccg ccgcccgacc tcgtcatggc tcatacactc gccggcaggg
     1561 catccaccga tgtgcgcgcc atgcctctgc tctcggaccg ggcttacgag cgcatccgcc
     1621 atgacatcat cacctgcgcc atcgcgccgg ggaccgagat ttccgaagca cagttaggcg
     1681 cgcactacaa gctcggcaag gcgccggtgc ggatggcgct gaaccgcctc gcgcacgacg
     1741 ggctggtgcg cgccatcccg cgccgcggct accgggtgat ggaagtgacc ttgaaggaca
     1801 tccacgacgt gttcgagttg cgcctcatgc tggagcccgc cgctgcgcgc atggctgccg
     1861 gaagagtgga tgcccagcga ttgcgcgccc tggatgaagt ctgccgcgcc ggctaccaac
     1921 ggggcgatgc gaggagcacc tcgcgcttcc tcgaggcgaa caaggcgttc cacgtggaga
     1981 tcgcgcgggc ggccggaaac gtccggcttg ccggcgccat cgagcagctg cacgacgaaa
     2041 tgacccgtct tcttcacctg ggcctgggcc tgcgcaaccg ttcccatgag atgcagcgcg
     2101 agcatcgctc gctggtgaag gcgctcgagc gcggcgacgg cgccaccgcg gagcgcatct
     2161 gccgcgagca gatcgaggcg gcgcgcagca tggtgctgtc ggcgatactg acctccagct
     2221 cggtgatgaa tctcgcgctc ggcgtcgagg gcccgcggtg agcgcgctcc cgcaattgcg
     2281 gcatctcgcc gaccgcatcg gcgcggcggt gggtcttgcg ccggacgcaa tggcgagcga
     2341 gatcaaggcg gcgctgcgcg atgcgaccgc cggggcgaac tggctgcctt cggagcggcg
     2401 gcgcgcgagc cacgagaact acgcgcggca cctgctttac ggcgatcccg acggccgctt
     2461 ttcgatactc gccatcgtct gggatcacgg ccagatgagc ccgatccacg gccatcattg
     2521 ctggtgcgcc gtgggcgtct atcagggcgt gctcaccgag acccgttacc gcgaaggtgc
     2581 gggcggcccg ccggtcccgg ttaacagcac gcgtcgcgct gctggcacgt tgagcttcga
     2641 tcctgccgcc tccgcgatcc accgcattgc caatgagagc ggcgtgctcg ccgtttccct
     2701 gcacgtctac ggaatcgcaa aggacggcat ttcaacggga gtcaaccgca tttatgcgta
     2761 agaccggtga gcagtaagca gtgagcggtg agccgtggta gaaatgcgaa cgcagttatc
     2821 ctatttccgc ttaccgctta ccgcttacgc cttaccaggg aataaacaat gaaggcagtc
     2881 gtactggaga aacacggcgg gctcgaggag ctcaaattcg taaaggactt tccggacccc
     2941 aagacggccg acgggcatgt ggtgatccgg gtcagggcga catccttcaa ctatcacgat
     3001 gtcttcaccg tccgcggcat gcccggcatc aaggtgccgc tgccgatgat catcggtctc
     3061 gacatggccg gtgaaatcct ggaagtcgga ccgggcgtgg acccgaggtg gaaaaagggc
     3121 gaccgcgtgc tggtgaaccc tcttaaccgg cagaagggcc tgatgggcga gatgatgcac
     3181 ggcggtctcg ccgagaaatg cctggtcgcg gagcaccagt tgatccgcat gcccgaaggc
     3241 gtcaccttcg aggaggccgc ctcgcttccg gtcgcctatg gcaccgcgca ccgcatgctg
     3301 atcacccaca agacggttaa aagcggcgac agggtgatcg tgctcggcgc gagcggaggc
     3361 gtcggcaccg gctgcgtgct gcttgccaag atgctgggcg ccgaggtgat cgcgtgcgcc
     3421 tccagcgagg acaagatcaa acggctgaag gagcttgggg cggatcacgt catcaactac
     3481 aaggagaccg atttttcgaa gtgggcggtc gagaaatacg gcaagccgca gcgccgaacc
     3541 tatgaggggg gcgtggacgt cgtcatcaat ttcaccggcg gcgatacctg ggcgccgacg
     3601 ctgcgctgcg tgaagcgcgg cggcaagatc ctggtctgcg gcgccaccgc cggctacgac
     3661 cccaaggagg acttgcgcta catctggagc ttcgagttgc aggtgatcgg ctcgaacagc
     3721 ttctacgacg acaatctcca ggcgctgatg ggtctgatcc aggaaggacg gatgaagccg
     3781 gtgatcgaca aggtgctgcc gctcgagcgc gctgccgagg ggctacgcct gatcgagagc
     3841 cgggaagtct tcggcaaggt cgtcgtcact ccctagaacc ggtgaacggt gaactgtgaa
     3901 cggtgaatgg aaaccggtga actgtaccgc aagtgggagg atgcccatgg tgcgggcttt
     3961 ttccgttcac tgttcgctgt tcactgttca cccataacca tgccgattcc ctgccctgag
     4021 ccgcgcggat atccgggcaa cctaggcctg ctgttcgccg agcacgcgca ctcggagcgc
     4081 ccggcgatca tcgagctgcg cgaaccgaag cggccgcgac cagtgagctt ccgcgagctc
     4141 aacgcgggct gcaacgcggt cgcgcgcggg ctggtcaagg cagggctcaa gccgggagat
     4201 cgtatcggga tcgtgtcgct taaccgcgtc gagttcgtca tgacattgct cggcgcgatg
     4261 cgcgccggcg ccgttcccgt gccggtcaac atcaagctac ccgccgacgc ggtcgcctac
     4321 atcctcaacg atgctggcgc ccggatgacg tttgccgagc gcgatctgaa acgcctcgtc
     4381 ccggccgcgc tgcgcgtggt ggaattcggt ggtgccttcg actcgtttct cgatccgggg
     4441 ccgttcgagg cgttcgagcc cgcgccagat tcgatcgcga tacaacccta cacctcgggc
     4501 tcgaccggcc agccgaaagg tgtgctgctc acccactacg gccagaactg gagccggcgc
     4561 atcctcgcct atacgcgcgg cacgaccgaa aaagacgtaa tcctggtcgc tgcgcccctc
     4621 tatcacaaaa acgcgttgaa cgcgatcaag caaggcctga ccgccggtgc aacgctcccg
     4681 ttgctgccgc aattcgacgt cgagcgctac atcgaggcga tcgagcgcca ccgctgcacc
     4741 gtcatctccg gcgtcccgac catgatggcg atggtgctct cgcgcaagga cctcctcgcg
     4801 cagattgatc catcctcggt gcgcaccatc atgatgggct cggcgccatc ctcgccccag
     4861 ctcgtgaagg agctgcggtt gcatttcttg aacgccgagc cgctggtggt ttacggcgta
     4921 accgaaggcg ggccggttcc gctcggtcct cacccggaag gcaagccgcg cccgccgggc
     4981 tcgatcggcg tgccctaccc cggcaccgag gcgaaactgg tgggcgggtc gggtccggat
     5041 gagggcgagc tcgtgctgag gaatcccggc gtgctgctcg ggtatcacaa tctgcccgaa
     5101 gagaccgcga aacggctgcg cgagggctgg tattacaccg gggacatctg ccgccgcgac
     5161 gccgacggtt tctattactt cgtgggccgt accgatgaca tgttcgtttc gggcggcgag
     5221 aacatctacc cgggagaagt cgaggccttg ctcgcgcgcc acccggccgt gcaccaggcc
     5281 ctcgtcatgc cgttcgacca cgagctgaaa ggccaggtgc cctatgcctt tatcgtgcga
     5341 cgggccggcg ctgcagtgac cgaagacgag atcaagcggt ttgcgctggc gaacgggccc
     5401 gcctaccagc accctagacg cgtattcttc ctcgaccagt ttccgctcgc cggcaccaac
     5461 aagatcgaca agcagaagct gcggcaacgg gtcgccgacg gcacgctgga acgaccacca
     5521 gccgccaagg gagcgctgtg atgaccgatc aaccccttac cgcgcaacag ctgcgcgaga
     5581 tcatgaccag ccggccgttt caccagtggc tcgggctcga tgtgttgaaa tccgacgaga
     5641 ccggcatcga aatcaaggct gcctggcgtg aggaatgggt ggtcaacccc gagcggcgct
     5701 acacgcatgg cggcatcctc gccgccctgg tggacgtggc agccgattac gcgctgtgcg
     5761 cgaagatcgg ccggccggtg ccgaccatcg acatgc
//
LOCUS       DBNGWZ010000072         5939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_6068, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000072 DBNGWZ010000000
VERSION     DBNGWZ010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_6068"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="AC16CX_01975"
     CDS             <1..238
                     /locus_tag="AC16CX_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3011308.1"
                     /translation="ADVICKYAKEKDCDQSFRGTRGLGSVSNLRLGAVATKVIHESHV
                     PVLLVKLSVFHRGAVLRCGMAGTWTRDWDKIAET"
     gene            362..1363
                     /locus_tag="AC16CX_01980"
     CDS             362..1363
                     /locus_tag="AC16CX_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011309.1"
                     /translation="MRTRRILSCSAAIVVAALTAAPAAAWEPSKPVEFVIPAGQGGGA
                     DQMARLTQGIVAKHNLMKQPLIPVNKSGGAGAEGFLDVKASKGDEHKIIITLSNLFTT
                     PLATGVPFNWKDLTPVAMLALDNFVLWVNTESPYKSPKEYLDAVKKAGPNKFKMAGTG
                     AKQEDQIITVAIEKAMGVKFTYVPFKGGGDVAVQLVGKHVDSTVNNPIEAVAHWRAGK
                     LRPLCVFENARMPYKDKVTETMSWNDIPTCRDAGIPMEYLMLRGIFMPAGVSKDVVDF
                     YVGFFRKVRETPEWKKFMQDGAFNPSFMTGKEYADWVAKAEDLHRDLMREAGFLAKK"
     gene            1468..1974
                     /locus_tag="AC16CX_01985"
     CDS             1468..1974
                     /locus_tag="AC16CX_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3011310.1"
                     /translation="MQNNNEPAQRAVRIITMDVIVAALIFALGALVIYDSNRLGSSWS
                     ADGPQAGYFPYYTGLLVCISGAVVFVQGLFKLKSDVKVFVRQDQFRQVLVILLPSAVY
                     VLGIQLIGIYVPSAVFIGLFMRIFGKYSWLRSVLVGLAVSVLAFVLFEVWFKIPLPKG
                     PLEQLIGY"
     gene            2002..3519
                     /locus_tag="AC16CX_01990"
     CDS             2002..3519
                     /locus_tag="AC16CX_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3011311.1"
                     /translation="MEELNALFHGFSIALTPFNLVLMFVGIVLGVIIGVLPGLGGANG
                     VAILLPLTFTMSPTSAIIMLSCIYWGALFGGAITSVLFNIPGEPWSVATTFDGYPMAQ
                     QGRAGEALTGAFTSSFVGALVAVLLFTFLAPLIASFALQFGPPEFFSVYLLTFCSFVG
                     TGKGNPFKIIASMMLGFAMAAVGIDIVTGQLRLTFGFTELLSGFHFLVAVIGLFGIGE
                     ILLSMEEGLSFSGHSAKINPKVVLQTWAKLPRYWATSIRSCIIGCWMGIIPGGATPAS
                     FMSYGVARKMSRDGDKFGAGQLEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLLIWGLQPGPLLFVEQKDFVWGLIASMYLGNIAGLIVVLTTVPLFAAILRVPFPII
                     APVIVVICAIGAFTVKNNMLDVWFMLMFGVVGYVFKKLDYPLAPMVLALVLGDAAESS
                     FRQSMLISQGDLTVFFSNWLVGGLTGLALFALFWPLIARGIAVLRTNRPRGAMTS"
     gene            3878..4183
                     /locus_tag="AC16CX_01995"
     CDS             3878..4183
                     /locus_tag="AC16CX_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3011312.1"
                     /translation="MTTVVTDNCQRCRFTDCVTVCPVACFHGDDEMLYIDPDVCIDCS
                     ACIPKCPVEAIYEDPIPEDKQHWIAINADRAPRLPVISKKQEPLPGAEERKKQLGFE"
     gene            4244..5626
                     /locus_tag="AC16CX_02000"
     CDS             4244..5626
                     /locus_tag="AC16CX_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3011313.1"
                     /translation="MSNLGTAQNPVRAAIVGSGPSGFYAAEALLASKQTVLVDVLERL
                     PSPFGLVRSGVAPDHPKLKQPIRVYQQIAESPRFRFLGNVTVGRDVSVEEMRGCYHVV
                     VFACGAETDRTLGISGEHLAGSHAATEFVGWYNGHPDYRDRVFDLSHETAVVIGQGNV
                     AVDVCRILAKTVDELRHTDIAKHALEVLAESRIRDIHMIGRRGPAQAKFTHHELRELG
                     DLVDCDPIVDPRDLELNPASREELAERRNRANIKSYEVLQGFASRPPPTRHRHCHIQF
                     FKSPKELKGTGRLERVVLARNRLEGGPSQQVARETGEVEELACGVLFRSIGYRGVPIA
                     GVPFDDGRGIFSNRDGRIVEGQNLVPGLYAAGWIKRGPTGIIGTNREDSVATVSSILA
                     DLPKINAGAKPGADGMKGLLESRGIRVVSYADWRKIDAAEVRRGAAAGKPREKFTRVE
                     EMLELLGHSD"
BASE COUNT         1121 a         1708 c         1903 g         1207 t
ORIGIN      
        1 ggcggacgtc atctgcaaat acgcaaagga aaaggactgc gaccagagct tcaggggcac
       61 ccgcgggctg gggtcggtgt cgaacctgcg gctgggagcg gttgcgacca aggtgatcca
      121 cgagtcccac gtgccggtgc tgctggtcaa gttgtctgtc tttcaccggg gcgccgtttt
      181 gcgctgcggc atggcgggga cctggacgcg ggattgggat aagattgcag aaacttgacg
      241 ggggtcaatt tttttcgcca gcggttggcg gatgatcgga tgtcagcaac cacaacaaaa
      301 cagggcggga gatgatgcac ggttgaagca gtgcagttca aaccacgagg aggagcaagt
      361 catgcgtacc cgacgtatcc tgtcatgcag tgcagcaata gttgtggccg cgctcacggc
      421 ggcgccggcg gcggcatggg agccgagcaa gccggttgaa ttcgtgatac cggccggcca
      481 aggcggcggc gccgaccaga tggcgcgcct gacgcagggc atcgtggcca agcacaacct
      541 gatgaagcag cccctcattc cggtcaacaa gtcgggcggg gcgggcgcgg agggcttcct
      601 cgacgtcaag gcctccaaag gcgacgagca caagatcatc attacgctgt cgaacctgtt
      661 cacgacaccg ctcgcgaccg gcgtgccgtt caactggaag gacctgaccc cggtcgcgat
      721 gctggccctg gacaacttcg tgctctgggt caacaccgaa tcgccctaca agtcgccgaa
      781 ggagtacctc gatgcggtga agaaagcggg gcccaacaag ttcaagatgg ccggcaccgg
      841 cgccaagcag gaggaccaga tcattacagt cgcgatcgag aaggccatgg gcgtgaaatt
      901 cacctacgtc ccgttcaagg gcggtggtga tgtggcggtg cagctcgttg gcaagcacgt
      961 ggactccacg gtcaacaacc cgatcgaggc ggtggcgcac tggcgggccg ggaagctgcg
     1021 ccccctgtgc gtatttgaaa acgcgcgcat gccgtacaag gacaaagtga ccgaaaccat
     1081 gtcgtggaac gatatcccga cctgcaggga tgccggcatc ccgatggagt acctcatgct
     1141 gcgcggcatt ttcatgcccg ccggcgtatc caaggacgtc gtcgatttct atgtcggttt
     1201 tttcaggaag gtgcgtgaaa cgccggaatg gaagaagttc atgcaggacg gtgccttcaa
     1261 tccgagcttc atgaccggca aagagtatgc ggactgggtc gcgaaggcgg aagacctgca
     1321 ccgcgacctg atgcgggaag cgggcttcct cgccaaaaaa tagcggcact tccggggacg
     1381 ggcgccatgc ccgtcccctt tttctctctg gggcggggcg tctgttcgcg cctcgtgttt
     1441 ctcgcgttga cctgagcagt ggctgatatg caaaacaaca acgaaccggc gcagcgcgcg
     1501 gtacgcatca tcaccatgga cgtcatcgtt gcggcgctga tcttcgcgct cggcgcgctg
     1561 gtgatctacg acagcaaccg gctcggctcg agctggagcg ccgacgggcc gcaggcgggg
     1621 tatttcccgt actacaccgg attgctggtg tgcatctcgg gcgcggtggt gttcgtgcaa
     1681 ggcctgttca agctcaagtc cgacgtgaag gttttcgtcc ggcaagatca gttcaggcag
     1741 gtgctggtga tcctgttgcc ctccgcggtc tatgtgctgg gaatccagtt gatcggcatc
     1801 tacgttccgt cagccgtgtt catcgggctg ttcatgagga ttttcggcaa atactcctgg
     1861 ctgcgcagcg tgctggtggg cctcgcggtc agcgtgctcg cattcgtgct gttcgaagtg
     1921 tggttcaaga tccccctgcc caaagggccg ctggagcagc tgatcggcta ctagcgcaag
     1981 cacggacgag gactatcgaa agtggaagaa ctcaacgcgc tgtttcacgg cttctcgatc
     2041 gcgctgacgc cgttcaacct ggtgctgatg ttcgtcggca tcgtgcttgg cgtcatcatc
     2101 ggcgtgctgc ccgggctggg cggggcgaac ggcgtggcga tcctgctgcc gctcacattc
     2161 accatgtcgc cgacctcggc gatcatcatg ctctcctgca tctactgggg agcgctgttc
     2221 ggcggcgcga tcactagcgt gctgttcaat attcccggcg agccgtggtc ggtggcgact
     2281 acgttcgacg gctatcccat ggcgcagcag gggcgggccg gggaggcgct gaccggcgcc
     2341 ttcacttcct ccttcgtggg cgccctggtc gcggtgctcc tgttcacttt cctcgccccg
     2401 ctgatcgcca gcttcgcgct gcagttcggc ccgccggaat tcttctcggt ctatctcctc
     2461 accttttgca gcttcgtcgg caccggcaag ggcaatccgt tcaagatcat cgcctcgatg
     2521 atgctgggct tcgcaatggc cgcggtcggc atcgacatcg ttaccggcca gttgcggctc
     2581 accttcggct tcaccgagct gctctccggc ttccatttcc tggtggcggt gatcggcctc
     2641 ttcggcatcg gcgagatact gttgtccatg gaagaagggc tatcgttctc cggtcacagc
     2701 gccaagatca accccaaggt cgtgctccag acctgggcca aactgccgcg ttactgggcg
     2761 acctcgatca ggagctgcat catcggctgc tggatgggca tcatcccggg cggggcgaca
     2821 ccggcctctt tcatgagcta tggcgtggcg cgcaagatgt cgcgcgatgg ggacaagttt
     2881 ggcgccggcc agctcgaggg cgtggtcgcg cccgaaaccg cggcgcacgc agccggcact
     2941 tccgccctgc tgccgatgat cacgctcggc atccccggct cgccgaccgc cgccgtgctg
     3001 ctcggggggc tgctgatctg gggcctgcag cccgggccgc tgttgttcgt ggaacagaaa
     3061 gactttgtat gggggctgat cgccagcatg tacctgggca atatcgccgg cctcatcgtc
     3121 gtgctgacca cggttccgct gttcgctgcc atcctgcgcg ttccgttccc gatcatcgca
     3181 ccggtgatcg tcgtgatctg cgccatcggt gccttcaccg tcaagaacaa catgctcgac
     3241 gtatggttta tgctgatgtt cggcgtcgtg ggctacgttt tcaagaagct tgactacccg
     3301 cttgcgccga tggtgctggc gctggtgctg ggggatgccg ccgagagctc gttccggcag
     3361 tccatgctga tctcgcaggg cgatctcacg gtctttttct cgaactggct ggtcggcggc
     3421 ctcacgggtc tcgcgttgtt tgcgctgttc tggccgctga tcgcgcgagg aatcgcggtg
     3481 ctgcgcacca accggccgcg cggtgcgatg acgagctagg tctccggcgc agccagatga
     3541 gcagagaagc ggctcgtcga ttcatgaccg catctcgaca ctggtgctgg cacgggcgcc
     3601 gccggtgcgc gccaagcgct gcagtagcgt caccgggctg caaatgcacc atttcgggat
     3661 ctcttccgga accgcagtcc tgaaacttgg ccgccccggt tcccttcgag gcgcgaatga
     3721 gacattacgt caagacggat ttcctgtccg taacaaccct ggttgtccat tgacgaaaac
     3781 cagttgaatg aacatcatgg atgcctaggt gccctccagt ggaccttgac agagcagcga
     3841 tctcgctctt cagttcttca cgaaaaaagg aggaaaaatg acgaccgtcg taaccgataa
     3901 ttgtcaacgt tgccgtttta ccgactgtgt cacggtatgt ccggtcgcct gttttcatgg
     3961 tgatgacgag atgctctaca tcgaccctga cgtgtgcatc gactgcagtg cttgtatacc
     4021 caaatgtccg gttgaagcga tttacgagga cccgatccca gaggacaagc agcactggat
     4081 tgcaatcaat gccgatcgcg caccccgact gccggtgata agcaagaaac aagaaccgct
     4141 tccaggcgcg gaagagagga aaaaacagtt agggtttgag taagccgaac ccctctctag
     4201 catcgcggtc ccgtcgcctt cctgctacgg gagacagcct ggcatgagta acctcggcac
     4261 agcacagaat cccgtgcgcg cggcgattgt tggcagtgga ccgagcggtt tctatgctgc
     4321 cgaggcgttg ctcgcatcga aacaaacggt tcttgttgac gtgcttgaac gccttccctc
     4381 gccctttggg ctggtgcgca gcggcgtagc cccggaccac ccgaagctca agcaacccat
     4441 acgcgtctat cagcagattg ccgaatcccc gcgatttcga ttcttgggca atgtcacggt
     4501 tgggcgtgac gtcagtgtgg aggagatgag gggttgctac cacgtcgtcg tgtttgcttg
     4561 cggcgcggaa accgaccgca ccctgggaat ttcgggggag cacctggcgg gcagccacgc
     4621 ggctacggag tttgttggat ggtataacgg ccaccctgac tatcgcgatc gggtgtttga
     4681 cctgtcgcat gagaccgccg tggtcatcgg acaggggaat gtcgctgtcg acgtctgccg
     4741 catactcgcc aagacagtgg acgaattgag acacacggat atcgccaagc acgccctcga
     4801 ggtattggcg gagagcagaa tccgtgacat tcatatgatt ggacgccggg gcccggcgca
     4861 agcgaagttc actcaccacg agctaagaga gcttggggac ctggtggact gtgacccaat
     4921 cgtagaccca cgtgatcttg agttgaatcc ggccagccgc gaggaactcg cggagagacg
     4981 taatagagcc aatatcaaga gctatgaagt gctgcagggg ttcgcttccc gaccaccgcc
     5041 cacacggcac cggcactgcc acatccagtt cttcaagagt ccaaaggaac tcaaaggcac
     5101 aggaaggctt gaacgggtgg tgctcgcgcg aaaccgtctg gagggagggc cttcgcagca
     5161 ggtcgcacgt gaaacgggtg aggtggagga gcttgcatgc ggcgtgctgt ttcgcagcat
     5221 cggctatcgg ggtgttccca tagcgggtgt tccttttgac gacggtcgag gcatcttttc
     5281 caaccgggat ggacgcatcg tcgaggggca gaacctcgtt ccgggcttat atgccgcggg
     5341 ctggatcaag cgtggtccga ccgggatcat tggcaccaat cgggaggata gtgtcgccac
     5401 ggtaagttcg attctggcag acttgccgaa gattaatgct ggcgcgaaac cgggcgcgga
     5461 tggaatgaag ggtctgttgg aaagccgcgg gattcgcgtg gtcagctacg ccgactggcg
     5521 gaagatcgat gcggcagagg tgcggcgcgg cgcagccgcc ggcaagccga gggagaagtt
     5581 cacccgggtg gaagagatgt tggagttact cggccactcc gattagacac accatgggga
     5641 tgcgtattgc accgtccccg gcggttacag atgccccgga tcgacttcgg ccctcggggg
     5701 cgtcccgtag ggtgggcgtt cggggccggt catttccacg aatctcgctg cggagcacag
     5761 cggacagttt ccggatttga tgtagatcaa acaatgggag cttcgagctg ttatggtgga
     5821 catctgtccg ttcaggccat gagggggaac gtgctatgag cactgggtcc gtggaggtcg
     5881 tgtatcgggg catttttcag aaaactttgg ccaagaacat ctgccgcggg attgttttt
//
LOCUS       DBNGWZ010000073         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_383996, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000073 DBNGWZ010000000
VERSION     DBNGWZ010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_383996"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="AC16CX_02005"
     CDS             complement(<1..953)
                     /locus_tag="AC16CX_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3011314.1"
                     /translation="MWRISYRLFHVVAALLHWGKRRFTAAGMLVLSGIVVTGAVGVDT
                     TQSVAYQAFGLLLALIAVSALCLPLLRVRVSAERELPRVATAGETFTYRLRVANLGGR
                     ALDGLSVFEDLADPRPSFEAFRAALRIPTYRAWKRMINAQRAAQVPEQPLPVLPPRGQ
                     AEIEVRAWALRRGTAHSDGLTLARADPLGLVKTLARVPRRSNFIVLPRRYSLPPLNLP
                     GSRRYQPGGVALATSVGDSEEFVGLRDYRPGDPLQRIHWKSFARAAKPIVKEYQDEYF
                     ERHALVLDTFMAARDEHSFEEAVSIAASFAYTIDTRECLLDL"
     gene            complement(1052..2011)
                     /locus_tag="AC16CX_02010"
     CDS             complement(1052..2011)
                     /locus_tag="AC16CX_02010"
                     /inference="COORDINATES: protein motif:HMM:NF040105.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011315.1"
                     /translation="MNDASSQNPAGTRALIAAIADNIGRVMSGQAGSIRKILAAFLAG
                     GHVLLEDNPGTGKTTMAKALARTTGADYRRVQFTPDLLPSDILGVSVFNPRDQSFHFH
                     AGPVFTQILLADEVNRASPRTQSALLEAMAERQVSIEGKTHALDDLFFVIATQNPVEF
                     RGTYPLPEAQMDRFAVRLALGYVSAEEEVDILNAQALEHPLERIGPRVNPDDVRRLKD
                     AVKAVRLSTELKRYAVDIVRASRDRPSVQLGASPRAALALARLAQALALIDGAEFVTP
                     DHIREMAVPVLAHRLMLDPQAKFSGMTARQVVEDMLGAVRAPA"
BASE COUNT          355 a          712 c          805 g          334 t
ORIGIN      
        1 agatcgagca ggcactcgcg ggtgtcgatc gtgtaggcga aggaggccgc gatggacacg
       61 gcttcctcga agctgtgctc gtcgcgcgcg gccatgaagg tgtcgagcac cagcgcgtgc
      121 cgctcgaagt actcgtcctg gtactccttg acgatgggct tcgcggcgcg cgcgaacgat
      181 ttccagtgga tgcgctgcag ggggtcgccc gggcggtaat cgcgcaggcc gacgaattcc
      241 tccgaatcgc cgacggaagt cgcgagcgcc acgccgccgg gctggtagcg ccgcgagccg
      301 gggaggttga gcgggggcag cgagtagcgc cggggcagca cgatgaagtt cgagcgacgc
      361 ggcacgcgcg cgagcgtctt caccagtccc agcgggtcgg cgcgcgccag ggtgagtccg
      421 tcgctatggg cggtgccccg gcgcaatgcc cacgcccgca cctcgatctc cgcctgcccg
      481 cgcggcggga gcaccggcag cggctgctct ggcacctgcg ccgcgcgctg cgcgttgatc
      541 atgcgcttcc acgcgcggta ggtgggaatc cggagcgcgg cgcggaacgc ttcgaagctt
      601 ggacgggggt cggcaaggtc ctcgaagacc gacagcccgt cgagcgcgcg gccgcccagg
      661 ttggccacgc gcaggcggta ggtgaaggtc tcgccggcgg tggcgacgcg cggcaactcg
      721 cgctccgccg acacgcgcac gcgcagcaac ggcaggcaca gcgcgctcac cgcgatcagc
      781 gcgagcagca ggccaaaggc ctgataggcg acgctctggg tggtgtccac gccgaccgcg
      841 ccggtgacca cgatgccgga gagcaccagc atgccggcgg cggtgaagcg gcgcttaccc
      901 cagtgaagga gcgccgcgac gacgtggaac aggcggtagg aaatgcgcca catcgaaaat
      961 caagaacagc tactagccgc agataaacgc cgattcttga tcctgtccgc tttcatctgc
     1021 gtgtacctgc ggtttcaagg gattgttggg gtcacgccgg cgcgcgcacg gcgccgagca
     1081 tgtcttccac cacctgccgt gccgtcatgc cggagaactt cgcctgcgga tcgagcatca
     1141 ggcggtgcgc cagcaccggc accgccatct cccgtatgtg atcgggggtg acgaactcgg
     1201 ctccgtcgat caatgcgagc gcctgggcga gccgcgccaa agcgagcgcg gcgcgcgggc
     1261 tcgcgccgag ctgcaccgag ggacggtcgc gcgaggcgcg cacgatgtcc accgcgtagc
     1321 gcttgagctc ggtggagagc cgcaccgcct tcaccgcatc cttcaaccgc cgcacgtcgt
     1381 cagggttgac gcggggcccg atccgctcga gcgggtgctc aagcgcctgc gcattcagga
     1441 tatcgacctc ctcctcggcg ctgacgtatc cgagcgcgag gcgcacggcg aagcggtcca
     1501 tctgcgcctc gggcagcgga taggtcccgc ggaactcgac ggggttctgg gtggcaatta
     1561 cgaagaagag gtcatcgagc gcgtgcgtct tgccctcgat gctcacctgg cgctcggcca
     1621 tcgcctcgag cagagcagac tgcgtgcggg ggctcgcgcg gttgacctcg tcggcgagca
     1681 agatctgggt gaagaccggg ccggcatgga agtggaacga ctggtcgcgc gggttgaaca
     1741 ccgacacccc gagtatgtcg gaaggcagca ggtcgggcgt gaactggacg cgccggtagt
     1801 cggcgccggt ggtgcgcgcg agcgccttgg ccatggtggt cttgccggtg ccggggttgt
     1861 cctccagcag cacgtgcccg cccgcgagga aggccgcgag aatcttgcgg atcgagccgg
     1921 cctggcccga catcacccgc ccgatgttgt cggcgatggc cgcgatgagc gcgcgcgtgc
     1981 cggcagggtt ttgactggaa gcgtcgttca ccgggaaacc ttcatggatg gtccattcag
     2041 gcaagcgccg tcaacgcttg cctgtgtaga actgcggcca gcgctcgagc acggcggggg
     2101 agtcgtcctt gtagacgtgg caggaattga gctggtaggg cttcttcgtc atcggcttgc
     2161 cggcgctgcg cgcaagccgc gcgcggttcg ttgcctggaa cgcggt
//
LOCUS       DBNGWZ010000074         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1769795, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000074 DBNGWZ010000000
VERSION     DBNGWZ010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1769795"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="AC16CX_02015"
     CDS             complement(<1..272)
                     /locus_tag="AC16CX_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3011316.1"
                     /translation="MAFLEIEDLQKRFAQTTAVEHVQLDVTRGEFVSLLGPSGCGKTT
                     TLRIIAGFESPSSGAIRLDGVDITHRPPNQRHVGMVFQSYALFPNMT"
     gene            complement(334..1125)
                     /locus_tag="AC16CX_02020"
     CDS             complement(334..1125)
                     /locus_tag="AC16CX_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011317.1"
                     /translation="MKRRRLGWWLLLLFGVLYFFVPLLGTFEFSLRLLRGVYSVAAYR
                     IVLADPRFGATVTYSTVLALATIVVGVVLVVPTAYWIQLRLPRLRPVVEFITLLPLVI
                     PAIVLVFGYLRIYNSASVLPLTGAERTTDLLLMFSYVTLALPYMYRAVDTGLRAIDVH
                     TLTEAAQSLGAGWSTILLRVILPNVRVAVLSGAFLTFAIVIGEFTMASLLDRPAFGPY
                     LQLIGANRAYEPSALAVISFAITWACMGLIQVFGRGAPGQVAGAH"
     gene            complement(1122..2024)
                     /locus_tag="AC16CX_02025"
     CDS             complement(1122..2024)
                     /locus_tag="AC16CX_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011318.1"
                     /translation="MAQGTRLAIGPSPTALTWVNQVSWAWVGVVPFFVFALMFLILPS
                     GFLVVGGFQDSQGHLTLRNILDLFQPTIVSSYLISIKVSAASAIGGALVGFALAYAAI
                     AGGLPTWVRPTLMTFSGVASNFAGVPLAFAFLATLGRTGLATALLIRIFGFNIYSAGF
                     NLLSFLGLTLTYMYFQIPLMVLIMAPAIDGLKREWREASANLGASVWEYWRYIALPIL
                     WPPLLGAALLLFANAFGAVATAYALTGSSLNIVTIVLYAQIRGDVLHNQNLGYALALG
                     MILITGLSNAAYIWLRGRSDRWLR"
     gene            complement(2142..>2380)
                     /locus_tag="AC16CX_02030"
     CDS             complement(2142..>2380)
                     /locus_tag="AC16CX_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011319.1"
                     /translation="WMEYLYSDEGQLGWLKGYCHPIRFQNLVSSRKVPADLLAKLPRA
                     DAYAKALFPTLEQQDRYKQVITKQWDTVVGANVK"
BASE COUNT          488 a          773 c          772 g          347 t
ORIGIN      
        1 gtcatgttcg ggaacagcgc gtaggactgg aacaccatcc cgacgtggcg ctggttcggc
       61 ggcctgtgcg tgatgtccac cccgtcgagg cggatcgcgc ccgacgaggg cgactcgaac
      121 ccggcaatga tgcgcagcgt cgtggtcttg ccgcacccgc tcgggcccag cagcgacacg
      181 aactccccgc gggtcacgtc cagctgaacg tgctcgaccg ccgtggtttg cgcgaatcgc
      241 ttctgaaggt cctcgatctc gagaaaggcc attccggctc caggccgccc cctctcgccc
      301 cgccctccgc tgaccgcccc gcgcggttct tcgctaatgc gcaccggcga cctgcccggg
      361 cgcaccccgc ccgaagacct gaatgagacc catgcacgcc catgtgatcg caaagctaat
      421 gaccgccagc gccgacggct cgtaggcgcg attcgcgccg atgagctgca ggtaggggcc
      481 gaacgctggt cgatccaaca gactcgccat cgtaaactcg ccgatgacga tggcgaaggt
      541 cagaaacgcg ccgctcaaca ccgcgacccg cacgttgggg agaatcacgc ggagcaggat
      601 cgtcgaccac ccggcgccca ggctctgcgc ggcctctgtg agcgtgtgga cgtcaatggc
      661 gcgcaacccg gtgtcgaccg cgcggtacat gtacggcagg gccagcgtca cgtagctgaa
      721 catcagcaac aggtccgtcg tccgctcggc gcccgtcaac ggcaagaccg aggcactatt
      781 gtagatccgc aggtatccga acacgagcac gatcgcggga atcaccaggg gcaggagcgt
      841 gatgaactcc acgaccggcc gcagccgggg caaccgcagc tggatccagt aggcggtcgg
      901 caccacgagg acgaccccca cgacgatggt ggcgagcgcc agcacggtcg aataggtcac
      961 cgtcgcgccg aaccgcggat ccgcgaggac gatccggtac gcggcgacgc tgtacacgcc
     1021 gcgcagcaag cggagggaga actcgaacgt ccccaggaga gggacgaaga aatacagcac
     1081 gccgaacagc aagaggagcc accatccgag gcgacggcgt ttcatcgcaa ccatcggtcg
     1141 ctgcgtccgc gcagccagat gtacgccgcg ttcgataggc ccgtgatgag aatcatcccc
     1201 aacgccagcg cgtagccgag gttctgattg tgcagcacgt ctccgcgaat ctgcgcgtac
     1261 aggacgatcg tgacgatgtt cagcgagctg ccggtcagcg catacgccgt ggcgacggcg
     1321 ccaaaggcgt tcgcgaagag gagcaaggcc gctccgagca gcggcggcca gaggatgggc
     1381 agcgcgatgt agcgccaata ctcccacacg ctcgcgccga gattggccga cgcttcgcgc
     1441 cattcccgtt tgagcccgtc gatcgccggc gccatgatca gcaccatgag cggaatctga
     1501 aaatacatgt acgtcagggt caagcccaag aaactcaaca gattgaatcc ggcgctgtag
     1561 atgttgaatc cgaagatcct gatgagcagc gcggtcgcga ggcccgttcg acccagcgtg
     1621 gcgaggaagg cgaaggcgag cggcactccc gcgaagttcg aggcgacgcc cgagaaggtc
     1681 atgagcgtcg gccgcaccca ggtgggcagg ccgcccgcga tcgccgcata ggcgagggcg
     1741 aagccgacaa gcgcgccgcc gatcgccgac gccgcactaa ccttgatgct gatcagatac
     1801 gagctcacga tggtgggttg aaacaggtcc agaatgttgc ggagggtgag atggccttgg
     1861 ctgtcttgaa agccgcccac cacgaggaaa ccgctgggca agatcaagaa catgagcgca
     1921 aagacgaaga aggggacaac gcccacccag gcccatgaca cctggttgac ccatgtcaga
     1981 gcggtgggcg aagggccgat ggcgaggcgt gttccctgtg ccatgaagat cttccgcgcc
     2041 ctagcccctc ccccggatca gcgggggagg ggtgagcggc aggatgtcac cttgaaaccc
     2101 gagctgtgcg gacgcagatg cgctggcgcg gccggtgaag gctatttcac attcgccccg
     2161 acgaccgtgt cccactgctt cgtgatgact tgcttatatc ggtcctgctg ctcgagcgtc
     2221 gggaagagcg ccttggcgta ggcatccgca cgcgggagct tggccagcag gtcggccggc
     2281 accttgcggc tgctcaccag gttctggaag cgaatcgggt ggcagtagcc cttgagccag
     2341 cccagctgcc cttcgtccga atacaggtac tccatccaca
//
LOCUS       DBNGWZ010000075         2796 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_258540, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000075 DBNGWZ010000000
VERSION     DBNGWZ010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_258540"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16CX_02035"
     CDS             complement(<1..529)
                     /locus_tag="AC16CX_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3011320.1"
                     /translation="MKARRLFAHLGSCAIAAAALALTAPARAAEPVEEPVILVAKPAL
                     RDRIYGATILIAKPVGNGQHIGFIVNRPTPVTLGRLFPDHGPSQKVTDPVYLGGPVSM
                     ETLFCLVQRQESPGGKAMQLTPDLYLVVERELVDRIIEAGAAHARFFAGLVLWAPGEL
                     RAEIERGFWYVLDADA"
     gene            complement(610..1620)
                     /locus_tag="AC16CX_02040"
     CDS             complement(610..1620)
                     /locus_tag="AC16CX_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011321.1"
                     /translation="MKTAAVNAIDCVGRATAFFGVFPALLMPATAATVHAQTYPTKPI
                     RIIVGSAPGGPIDLTARITAQKLAEALGQPVVADNRTGAGGTIGTEYVARAAPDGYTL
                     GMGSAATLCITPHLYSKIGYDTLRDFAPVSTVAAISFVLVVHPSLPVKSVREFITLAK
                     SKPGELHFGSAGSGSVTHLAPELFKSMAGIRAVHVPYKGAGPALIDLMAGQLQFMLNS
                     IPTSVPHIASGRLRALGVSSTKRSPLLPDLPTISEAALPGYEASTWFGLVAPAATPRD
                     IVAKLNSVVVKSLTDTATRQRFLAQGLEPISNSPEEFSSLLRSELPKWGKIVKISEAK
                     VD"
     gene            complement(1617..2480)
                     /locus_tag="AC16CX_02045"
     CDS             complement(1617..2480)
                     /locus_tag="AC16CX_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3011322.1"
                     /translation="MSTQYRFARVGDLDIHYDLADYTVPWSATPPETVLLYHGYARNM
                     LFWRPWVPLLAADYRVLWFDARGCGDTTKPPAGSRMSFSQLAGDAIGLMDQLGIERVH
                     WVGESSGGIVGMTAALDYPDRIATLTLCDTPFKRSASIATTYTLGEASRAAAFDKHGV
                     GGWCRKTLSYRLDTTKASPALCEWYIRQMDRTPKHVAVAMDGAVAEGDLWPRLPEIRT
                     PTLILAGEKSPISQEAEVKAMHQRMPAAGLVVFAGYGHGVNLLAPERCVAEVRKFLAA
                     RKKTAAPAAVA"
     gene            complement(2533..>2796)
                     /locus_tag="AC16CX_02050"
     CDS             complement(2533..>2796)
                     /locus_tag="AC16CX_02050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3011323.1"
                     /translation="GGFGTLDELFELLTLVQTRKMSKPMPIVLFGTEYWRQVVDFDAL
                     VRHGTINAQDVELVHRTDSVEDAYNWIVLQLAEHALGQPGPML"
BASE COUNT          465 a          907 c          929 g          495 t
ORIGIN      
        1 ttgcatcggc gtcgagcacg taccagaaac cccgctcgat ttcggcgcgc aactctccgg
       61 gcgcccacag caccagcccg gcgaagaacc tggcatgggc ggccccggcc tcgatgatgc
      121 gatccacgag ctcgcgttcg accacgagat agaggtcggg cgtgagttgc atcgccttgc
      181 cacccgggct ttcctggcgc tgaaccaggc agaacagggt ctccatgctg accggtccgc
      241 cgagatagac cgggtcggtc actttctgcg aaggcccgtg gtcgggaaag agcctgccga
      301 gcgtcaccgg cgtcggccgg ttgacgatga atccaatgtg ctggccgttg ccgaccggct
      361 tggcgatgag gatcgtcgcg ccgtagatcc ggtcgcgcag ggcgggcttc gcgacgagta
      421 tcaccggttc ttcgacgggc tccgccgcgc gcgccggggc ggtgagggcg agcgcggcag
      481 ctgcgatcgc gcaggatccc agatgcgcga agaggcggcg ggctttcatt tcagcaatct
      541 ccattgcaga gagtttaccc tcaggtttgc tgcgtcgcaa caatcatcac ccgtcacact
      601 ttgtccattt caatcaacct tcgcctcgga gatcttgacg atcttccccc actttggcag
      661 ctcggagcgc aacaggctgc tgaattcttc gggcgagttg ctgatcggct cgagtccctg
      721 agccagaaac cgctgcctgg tggcggtgtc cgtcaggctt ttcacgacca ctgaattcag
      781 cttcgcgacg atgtcgcggg gagtcgcggc gggcgcgacc agtccgaacc acgtgctcgc
      841 ctcgtaaccc ggcaaggcag cctcgctgat cgtgggcaaa tcgggcagca gcggcgagcg
      901 tttcgtgctg gagacgccca gcgcgcgaag cctgcccgac gcgatatgcg gcactgaagt
      961 gggaatgctg ttgagcatga actgaagttg tccggccatc aggtcgatca gcgcgggacc
     1021 ggcgcctttg tagggaacgt gcacggcacg gatcccagcc atgctcttga acagttccgg
     1081 cgcgaggtgc gtcaccgatc cgctcccggc cgagccgaag tgcagctcgc cgggttttga
     1141 tttcgcgagg gtgatgaact ccctgaccga tttcaccggc agcgagggat gcacgaccag
     1201 cacgaacgaa atggccgcca ccgtgctcac gggcgcgaaa tcccgcagcg tgtcgtagcc
     1261 gattttcgag tacaggtggg gcgtgatgca aagcgtcgcg gcgctgccca tcccgagcgt
     1321 gtagccgtcg ggtgcggccc gggcgacgta ttccgtgccg atcgtcccgc ccgccccggt
     1381 acgattgtcc gcgacgaccg gttgccccag cgcctcggcg agcttctggg ccgtgatgcg
     1441 ggcggtcagg tctatcggcc cgcccggggc ggagccgacg atgatgcgga tcggtttcgt
     1501 gggataggtt tgggcatgga cggtcgccgc agtggctggc atcagcagcg ccgggaacac
     1561 gccgaaaaag gcggtcgcgc ggccgacgca gtcaatggcg tttactgcgg cggttttcat
     1621 gccactgcgg cgggcgcggc cgtctttttt cgcgcggcaa ggaatttccg gacctcggcc
     1681 acgcagcgtt cgggtgccag caggttgacg ccgtgcccgt atccggcgaa aacgacgagc
     1741 ccggccgcag gcatgcgctg gtgcatggcc ttcacctccg cttcctgcga gatcgggctt
     1801 ttctctcccg ccaggatgag cgtcggcgtc cggatttccg gcaagcgcgg ccagaggtcc
     1861 ccttccgcga cggccccgtc catggccacc gcaacgtgct tcggggtgcg gtccatctgc
     1921 cggatgtacc actcgcacaa ggccggcgag gctttcgtcg tgtcgaggcg gtaggaaagc
     1981 gtcttccggc accagccgcc gacgccgtgc ttgtcgaagg cggccgcgcg gctggcttca
     2041 cccaaggtat aggtggtggc gatactggcg gaccgcttga acggcgtgtc gcacagggtg
     2101 agcgtcgcga tgcggtcggg gtaatcgagc gcggcggtca tcccgacgat cccgccggag
     2161 gactcgccca cccagtgcac gcgctcgatt cccagctggt ccatgagacc gatggcgtcg
     2221 ccggcgagct ggctgaagga catccggctt ccggcgggcg gcttggtggt gtcgccgcaa
     2281 ccgcgcgcgt cgaaccacag cacgcggtag tccgccgcga gcaacggcac ccacggccgc
     2341 cagaacagca tgttgcgcgc gtagccgtga tacagcagga cggtttccgg cggcgtcgcg
     2401 ctccacggca ccgtgtaatc ggcgagatcg tagtggatgt cgagatcacc gacgcgggca
     2461 aaccggtatt gcgtgctcat gttctccttc cctctgcttt tattacgacc cacgatccac
     2521 gacccacgaa attcaaagca tcgggccagg ctggccgagg gcgtgctcgg cgagctgcag
     2581 aacgatccag ttgtaggcgt cttccaccga atcggtgcga tgcaccagct ccacgtcctg
     2641 cgcattgatc gtgccgtggc gcacgagcgc gtcgaaatcc accacctgcc gccagtactc
     2701 ggtgccgaac aggacgatcg gcatcggctt gctcatcttg cgcgtctgca ccagcgtcag
     2761 cagctcgaac agctcgtcga gcgtgccgaa cccgcc
//
LOCUS       DBNGWZ010000076         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2021794, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000076 DBNGWZ010000000
VERSION     DBNGWZ010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2021794"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /gene="meaB"
                     /locus_tag="AC16CX_02055"
     CDS             <1..878
                     /gene="meaB"
                     /locus_tag="AC16CX_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3011324.1"
                     /translation="EAGRAIMRAIHPHLGRAQVLGVTGAPGAGKSTLVSELIGEFTRR
                     ARSVAVVAVDPSSPFSGGAVLGDRIRMERHALAGRVFIRSLASRGHLGGLSRAVFRVV
                     DVLDAAGFDVIIVETVGAGQSEVEITDVADIRVVVCPPGLGDDIQAMKAGILEIADIF
                     VVSKCDLPLADKTEQELRYMLGLRKARPREPQILRVTATSGEGVAELADAIESLARAV
                     GRRRHEGARGRTRMMIAQEAGNAIRERLRAAQSEALDRLCEAVDRGELGHEEALRAAI
                     RLAMDGGTETEGGNN"
     gene            complement(886..1803)
                     /locus_tag="AC16CX_02060"
     CDS             complement(886..1803)
                     /locus_tag="AC16CX_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3011325.1"
                     /translation="MTLKQLETFYWVCRLGGFAAAAKHLHSTQSAVSMRIQDLESSLG
                     VPLFDRSQRSARLTAKGQELVKYAEQLMTVATEIRHRIGDPRVLWGTVRLGVTEFVAV
                     TWLPKLVTAINENYPGIVLELDVDLTLNQLRKLRDGDIDVAVLPGPIAEPGLANVPLG
                     SVEFAWMASPKLGVPRKRLTPRDLHAWPLLTLTRESNLHKLLGTWFEQNDATARRVDV
                     CNSIGVLAALTMAGLGVSFLPRGHFAAEIESGRLQVLDLTPRLADLEYFAVFEKRQTQ
                     PLSQTIAQLAQQCSSFRKTGARPRARNRR"
     gene            1906..>2285
                     /locus_tag="AC16CX_02065"
     CDS             1906..>2285
                     /locus_tag="AC16CX_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011326.1"
                     /translation="MLVGEFGKFIAQTRYEGLPANVVEAVKLRVLDCLAAALAGCHMG
                     CHRQLLPLLGGAGPATGWGVGRKFSVRDATLLNSFLSHALYLDDGSRFTGGHPSSVVI
                     PSAVALAETERATGRRLIAAVAAG"
BASE COUNT          365 a          735 c          806 g          379 t
ORIGIN      
        1 ccgaggcggg tcgcgcgatc atgcgggcaa tccaccctca tctcggccgc gcacaggttc
       61 tgggtgtcac cggcgctccc ggcgccggca aatcgacgct ggtgagcgaa ctgatcgggg
      121 agttcacccg ccgcgccagg tcggtcgcgg tggtggccgt cgacccctcg agcccgttct
      181 cggggggggc cgttctgggg gaccggatcc gcatggagcg acacgcgctc gccgggcgcg
      241 tattcatccg ctcgctcgcc tcgcgcgggc atctgggcgg gctttcgcgc gccgtcttcc
      301 gcgtggtcga tgtgctggac gcggccggct tcgacgtgat catcgtcgaa accgtcggcg
      361 cgggccagtc cgaagtggag atcacggacg tcgccgacat acgggtcgtg gtgtgcccgc
      421 ccggtcttgg cgacgacatc caggcgatga aagccggcat cctggaaatc gccgatattt
      481 tcgtcgtcag caagtgcgac ttgccgctcg ccgacaagac cgagcaggag ctacgctaca
      541 tgttgggcct cagaaaggcc cgcccccgcg agccgcagat cctgcgcgtg accgcgacca
      601 gcggcgaggg cgtcgccgag ctcgccgacg cgatcgaatc gctcgcccgg gcggtcgggc
      661 gccggcgcca cgagggagca cgcggccgca cgcgcatgat gatcgcgcag gaggcgggaa
      721 atgccatccg cgagcgcttg cgcgcagcgc aaagcgaggc gctggaccgg ctttgtgaag
      781 cggtggatcg cggcgagctt gggcacgagg aagcgctccg ggcagcgatc cgtcttgcaa
      841 tggacggcgg cacggaaacc gaaggcggaa acaattgagg cgtgttcatc tgcggtttcg
      901 agccctcgga cgcgcgccgg ttttccgaaa gctgctgcac tgttgggcga gttgcgcgat
      961 ggtctgtgac aggggctggg tctggcgttt ctcgaagacc gcaaagtatt cgagatccgc
     1021 gagcctgggc gtgagatcca ggacttgcaa ccgcccgctt tcgatttcgg ccgcgaaatg
     1081 cccgcgcggc aggaaactca cgccgagtcc cgccatggtc agcgcggcga gcacgccgat
     1141 gctgttgcag acgtcgaccc ggcgggcggt ggcatcgttc tgctcgaacc aggtgcccag
     1201 cagcttgtgc aggttggact cacgggtgag cgtcaggagc ggccacgcat gaagatcgcg
     1261 gggggtgagc cgcttgcgcg gcacgccgag cttggggctc gccatccagg cgaactcgac
     1321 cgatcccaag ggcacgttgg cgagccccgg ttcggcaatc ggtcccggca gcacggcgac
     1381 gtcgatgtcg ccgtcccgca gcttccggag ctgattcagc gtcaggtcga cgtcgagttc
     1441 gagcacgatg ccgggataat tctcgttgat ggcggtaacc agcttcggaa gccaggtgac
     1501 ggcgacgaac tcggtcaccc ccagcctgac tgtgccccac agcacccgcg ggtcgccgat
     1561 gcgatgccgg atttccgtgg cgaccgtcat caactgctcg gcgtacttga cgagttcctg
     1621 ccccttggcc gtcagccgcg cggaacgctg gctgcgatcg aagagcggga cacccaggct
     1681 gctttcaagg tcctggatgc gcatggatac cgcggactgc gtcgaatgca ggtgcttggc
     1741 ggcggcggcg aacccgccga gccggcagac ccagtaaaac gtctccagtt gcttgagcgt
     1801 catggcatca gcagacttga ttgagcttat ggaaataata tcacctcggc ttatctgtca
     1861 tgcgcggcca gccccggcgg caagcgcggt catgagtccg tccccgtgct ggtaggcgaa
     1921 ttcggaaaat tcatcgcgca gacgcgctac gagggcctgc cggccaacgt ggtcgaggcg
     1981 gtcaagctgc gcgtgctcga ttgcctggcc gcggcgctcg cgggctgcca catgggctgc
     2041 cacaggcaac tgctgcccct gctcggcggc gccgggccgg cgaccggctg gggcgtgggg
     2101 cgaaagtttt cggtgcgcga cgcgaccctg ctcaacagtt tcctgtcgca tgctctgtat
     2161 ctcgacgacg gctcgcgctt caccggaggc catccgtcgt cggtagtcat cccgagcgcg
     2221 gtggcgctcg ccgagacgga acgggcgacg gggcggcgcc tgatcgcggc ggtcgccgcc
     2281 ggcta
//
LOCUS       DBNGWZ010000077         4995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2777646, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000077 DBNGWZ010000000
VERSION     DBNGWZ010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2777646"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16CX_02070"
     CDS             complement(<1..222)
                     /locus_tag="AC16CX_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011327.1"
                     /translation="MEERSKAWLSFLLSTFAFVLGCALAPATSAQQYPARTIRLIAHS
                     SPGGTSDILGRLVAQKLTEALGQQVVVENR"
     gene            complement(303..1040)
                     /locus_tag="AC16CX_02075"
     CDS             complement(303..1040)
                     /locus_tag="AC16CX_02075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011328.1"
                     /translation="MAFVTGASQGLGAEIAVALAREGCDVAVSSRRIERLSDTLGRIE
                     SAGARGVPLELDLRSQSSMERALEEAVGALGRIDVLVNNAAVTLRQRALDMTREEWDE
                     LMAVNLKGTFFMSQLAGRHLVASRRPGCIVSLASTHGLVGYAQRLAYGVSKAGIIQMT
                     RMLAIEWAEHGIRVNAVAPGTLDTPTRTEYFAAHPDAKQAMIDRIPFRRFGTPEEVAA
                     SVCYLASPRAAYITGQTLVLDGGLTSY"
     gene            complement(1092..1940)
                     /locus_tag="AC16CX_02080"
     CDS             complement(1092..1940)
                     /locus_tag="AC16CX_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3011329.1"
                     /translation="MKYRFLLIQAFHLPEDSPYLHRAVKGTKEARLMNYERVAPLLED
                     VEWELHPGPLAPYGDWPVESREEFALVAAGRLPIVREACESGKYDAIVLLGGGEPGFQ
                     ESREIARRYNIPVTACAWSQMHVASLLGNRFSIIDHAEIHNMYYRDLVVMHRFDGRCA
                     SIRNINFPLPRPAHPDVPNIQDEKARALRGERSAMLEAAIVEAIAAIEEDGAEVITFG
                     CSATFWMQPFLQQRLNDMGWEAPVLEGYCCAIALAKSMVDLGLSASGLTYPGDHPKKW
                     RRRKIF"
     gene            complement(2045..3022)
                     /locus_tag="AC16CX_02085"
     CDS             complement(2045..3022)
                     /locus_tag="AC16CX_02085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011330.1"
                     /translation="MRSLSQCILMVLAGWVVAAAQAQDYPSRPIRMIVPFGPGGSSDF
                     TARIIQPKMIEALGQQVVVDNRAGASGNVGVEIAARAPADGYTYFLGNIGTMAINPSV
                     FPKFPVRPLRDFISVTMVVDVPGALAVHPSIPARSLKEFIDYAKSRPGQLNYGSSGAG
                     SAQRLAYEVFMSKAGINLVHVPYKGGAGAATTAVLSGEVSSTMVTVASFIPHVKTGKL
                     RVLAVVAPTRVPQLPDVPTFAESGFPELTVGSWQGMYLPAGTPKPIVQKLFRAMSRVL
                     ADPWVVERFTVGGAIVLPSKSPEEFARFMKEQNALWAKTVKAIGAVGEQ"
     gene            complement(3074..3427)
                     /locus_tag="AC16CX_02090"
     CDS             complement(3074..3427)
                     /locus_tag="AC16CX_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011331.1"
                     /translation="MPITGKWIFTAAMDVDPDKEALFNEVYDQEHVPNLSKVPGVISM
                     TRVKLDTLRITMGGETNIVDPRGVPRYACIVELESPDVLVSPAWAKAVDAGRWPAQVR
                     PYTRNRHHTLHKVMK"
     gene            3714..4022
                     /locus_tag="AC16CX_02095"
     CDS             3714..4022
                     /locus_tag="AC16CX_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011332.1"
                     /translation="MTDFATAGWVVATAAWVAFALYAFWPAGWVWRERLMRCPETGAI
                     TLVGIAPASRRVENAPEVEVQRCGLWPEKMDCARGCLARYREISPDYRVNVGALRPYE
                     "
     gene            complement(4113..>4995)
                     /locus_tag="AC16CX_02100"
     CDS             complement(4113..>4995)
                     /locus_tag="AC16CX_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3011333.1"
                     /translation="HVANVWVWKHWEAKGVEIRFARHRGGRLPLEAFLEKMDGRTRIV
                     STAYVTYGNGYRVNLPELGRVCRERDVRLVVDGVQAAGILSTPLNELGADIIAIGGHK
                     GLLGLTGSGVVYCREELVNQLRTDFVKQPAAAEPAARAHVVSQFDYVRSAHRFEGGNP
                     NFLGIRVFRHGAEFIRSIGLKHIEQRVRALTTTCLKLLQAAGLKGLTPAAWEERAQIV
                     NVLVPNAAELMERLKEKHRVVANVKDDALRLSMSFFNNEEDLERAVHALKRELAGKPA
                     PARPLPAAASALDIGPD"
BASE COUNT          846 a         1672 c         1576 g          901 t
ORIGIN      
        1 gcggttctcg accaccacct gctggccgag cgcctcggtc agcttctgcg ccaccaggcg
       61 ccccaggatg tcgctggtgc cgccgggcga ggagtgggcg atcaacctga tcgtccgcgc
      121 cgggtactgt tgcgcgctcg tcgcaggcgc gagcgcgcag ccaaggacaa aggcgaaagt
      181 cgaaaggaga aatgaaagcc aagccttact acgttcttcc atcacttccg cctttcgcct
      241 ttcgccttga tcttcatccc tccgccctcg tccctcgaca ctcgtccctc gacactcgtc
      301 agtcagtagg acgtgagccc gccatccagc accagcgtct ggccggtaat gtacgccgcc
      361 cgcggactcg ccagatagca caccgacgcc gccacctcct ccggcgtgcc gaagcggcgg
      421 aacgggatgc ggtcgatcat cgcctgcttc gcatcgggat gcgccgcgaa gtactcggtg
      481 cgcgtcggcg tgtcgagggt gccgggcgcg acggcgttga cgcggatacc gtgttccgcc
      541 cactcgatcg ccagcatccg cgtcatctgg atgatcccgg ctttggacac gccataggcc
      601 aggcgctgcg cgtatccgac cagtccgtgg gtcgaggcga ggctcacgat gcagcccggc
      661 cgccggctcg cgacgaggtg ccgccctgcc agctggctca tgaaaaaagt gcccttcaga
      721 ttgacggcca tcaactcgtc ccattcttcg cgcgtcatgt cgagtgcccg ctggcgcagc
      781 gtgaccgcgg cgttgttgac caggacatcg atgcgcccga gggcgccgac ggcctcttcc
      841 agcgcgcgct ccatgctcga ttgcgagcgc agatcgagct ccagcggcac gccgcgcgcg
      901 cctgccgatt cgatgcgtcc gagcgtgtcc gacagtctct cgatgcgccg tgaggagact
      961 gcaacatcgc agccctcgcg cgcgagcgcg accgcgatct ccgcgccgag cccttgcgac
     1021 gcgccggtca cgaatgccac ccgcctttca tcagcacttc gagtcacaag gcaccagtca
     1081 cgagtcatgc atcaaaagat tttcctgcgc cgccactttt tcgggtgatc accaggataa
     1141 gtcaacccgc tcgcggagag acccaggtcc accatcgact tcgcgagcgc gatcgcgcag
     1201 cagtagccct cgagcaccgg cgcttcccac cccatgtcgt tcaagcgctg ctgcaggaac
     1261 ggctgcatcc agaacgtcgc ggaacagccg aatgtgatga cctcggcgcc gtcctcctcg
     1321 atcgccgcga tcgcctcgac gatggcggcc tccagcatcg ccgagcgctc gccgcgcagt
     1381 gccctcgcct tctcgtcctg gatgttgggg acgtcggggt gcgccggccg cggcaggggg
     1441 aaattgatgt tgcggatcga ggcgcagcgg ccgtcgaagc ggtgcatgac gacaagatca
     1501 cggtagtaca tgttgtggat ctcggcgtgg tcgatgatgc tgaagcggtt gccgagcagc
     1561 gatgccacgt gcatctgcga ccacgcgcac gccgtgacgg gaatgttgta gcgccgcgcg
     1621 atctcgcgcg attcctggaa tccgggctcg ccgccgccga gcagcacgat ggcgtcgtat
     1681 ttcccgctct cgcacgcctc gcgcacgatc ggcagccgcc cggccgccac cagcgcgaac
     1741 tcctcgcggc tctccaccgg ccagtcgccg tacggcgcga gcgggccggg gtgcagctcc
     1801 cactcaacgt cttcgagcag cggcgcgacg cgctcgtagt tcatgagtcg cgcttccttc
     1861 gttcccttca ccgcccggtg caggtacggc gagtcttccg ggaggtgaaa cgcttggatg
     1921 aggaggaaac ggtatttcat cgtctcaact gcgtgagtcg tgagtagtga gtggactgac
     1981 ccccttcacc atgtcacgcg tcaccatgcc acggttcatc acgtcacgat gtgttcgtcc
     2041 tcccttactg ctcccccacc gcgccgatcg ccttcacggt cttcgcccag agcgcgttct
     2101 gctccttcat gaatctcgcg aactcctcgg gcgacttgct gggcagcacg atcgcgccgc
     2161 ccacggtgaa gcgctcgacg acccacgggt ccgccagcac gcggctcatc gccctgaaca
     2221 gcttctgcac gatcggcttc ggcgtgcccg cgggaaggta catcccttgc catgagccga
     2281 cagtgagctc gggaaaaccc gattccgcga acgtcggcac gtcgggcagt tgcggaacgc
     2341 gggtgggcgc gaccacggcg agcaccctca gcttgcccgt tttcacgtgc gggataaacg
     2401 aggcgaccgt caccatcgtg gacgacacct cgcctgaaag caccgccgtg gtcgccgcgc
     2461 ccgcgccgcc cttgtagggg acgtgcacga ggttgatgcc ggccttgctc ataaacactt
     2521 cgtacgcgag gcgctgggcg ctgccggcgc cggaggagcc gtagttgagc tgaccagggc
     2581 ggctcttcgc gtagtcgata aattccttga gcgagcgcgc gggaatcgac ggatgcaccg
     2641 ccagcgcacc cggcacgtcc accaccatcg tcacgctgat gaagtcccgc agtggccgga
     2701 ccgggaactt cgggaacacg ctcgggttga tcgccatggt gccgatgttg ccgaggaaat
     2761 acgtgtagcc atcggccggg gcgcgcgccg cgatctcgac gccgacgttg ccggacgcgc
     2821 ccgcgcggtt gtccaccacg acctgttgtc ccagcgcttc gatcatcttc ggctggatga
     2881 tgcgcgcggt gaaatccgag gatccgccgg gtccgaacgg cacgatcatc cgtatcggcc
     2941 ggctcgggta gtcctgggcc tgggcggcgg cgaccaccca tccggccaga accatcagga
     3001 tgcattgcga cagggatctc atgcgctccc tcctttcatc ttctcagggg acctgggcag
     3061 ggtcgtcact gcgtcatttc attaccttat gcagggtatg gtgacgattt ctcgtgtagg
     3121 gccgtacctg cgccggccag cgtcccgcgt cgaccgcctt cgcccatgcg gggctcacca
     3181 gcacgtccgg gctctcgagc tcgacgatgc aggcatagcg gggaacgccg cgcggatcga
     3241 cgatgttggt ttcgccgccc atcgtgatgc gcagcgtgtc gagcttgacg cgcgtcatcg
     3301 agatcacgcc cggcaccttg ctgaggttcg gaacgtgctc ctggtcgtaa acctcgttga
     3361 aaagggcctc cttgtccgga tcgacgtcca tggcggccgt gaagatccat ttgccggtga
     3421 tgggcatgcg cttcctttcc ttcacgggat gattgcagga tgagcatcct agcaggaaag
     3481 gaagagtgac gaggtactgg tgacgattaa cggtgacgaa aaccaaaggc taacaaaggt
     3541 ccctccttgg aaaggaaaga agccaacctc gtctttccgt cacctgtatc cgtcaccctc
     3601 acctcgtcac ccgcatctcg tcacgcccca tgaaactttg atctagctca aatccgaagc
     3661 ccgtttccgg ccgcagacta gcgactcgaa gagagccttg gtaaggaggc cctatgacgg
     3721 actttgcaac agcgggatgg gtggtggcga ccgcagcatg ggtggcgttt gccttgtatg
     3781 cgttctggcc ggccgggtgg gtttggagag agcggctgat gaggtgcccc gaaacggggg
     3841 cgatcaccct ggtcggcatc gcgccggctt cgcggcgcgt cgagaacgcg cctgaggtcg
     3901 aggtccaacg gtgcggattg tggccggaaa aaatggactg cgcccgcgga tgcctcgcgc
     3961 gctacagaga gatctcgccc gattacaggg tcaacgtggg tgcgttgagg ccttacgaat
     4021 agctcttgtg ggacagcatt aagaggccgt ttcaaaatga ccgaaacggc cttgatggac
     4081 ggcggtatcc cctccgctac cgtctctaag gttcaatccg ggccgatgtc cagcgctgaa
     4141 gccgcggcgg gcaaaggccg cgcaggagcg ggcttaccgg cgagctcgcg cttgagcgcg
     4201 tgcaccgccc gctccagatc ctcttcgttg ttgaagaagc tcatcgacag ccggagcgcg
     4261 tcgtccttga cgttggcgac gacgcggtgt ttctccttca gccgctccat gagctccgcg
     4321 gcgttcggca ccagcacgtt gacgatctga gcgcgctcct cccatgccgc cggcgtaagg
     4381 cccttcagtc ccgccgcctg gagcagcttc aagcaggttg tcgtgagcgc gcgcacgcgt
     4441 tgctcgatgt gcttgagccc gatcgaccgg atgaattcag cgccgtgccg gaacacccgg
     4501 atccccagga agttgggatt gccgccctcg aaacggtgcg cgctccgcac gtagtcgaac
     4561 tggctcacga cgtgggcgcg cgccgccggt tccgccgccg ccggctgttt cacgaagtcc
     4621 gtccgaagct ggtttaccag ctcctcgcgg cagtagacca ccccggagcc ggtcaggccg
     4681 agcaggccct tgtggccgcc gatcgcgatg atgtcggcgc ccagctcgtt gagcggtgtc
     4741 gagaggattc cggcggcctg aacgccatcc accacgaggc ggacgtcgcg ctcgcggcag
     4801 acgcggccga gctccggcag gttcacgcgg tagccgttgc cgtaggtcac gtaagccgtg
     4861 gacacgatgc gggtgcgacc gtccattttc tcgagaaaag cctcgagcgg gagccgcccg
     4921 ccgcggtggc gggcgaaacg gatctcgacg cctttcgctt cccagtgctt ccacacccag
     4981 acgttcgcga cgtgc
//
LOCUS       DBNGWZ010000078         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_384858, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000078 DBNGWZ010000000
VERSION     DBNGWZ010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_384858"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16CX_02105"
     CDS             <1..425
                     /locus_tag="AC16CX_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024106.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HIR3011334.1"
                     /translation="AALDAGFPCGGWATEDRVAEDGTIPAHYPLAELPGGGYEERTLQ
                     NVLDSDGTVILHFGVLEGGTRQTQDCCKAHGKPHEIIDASLVAPEEAAPRLAAFIERH
                     RIAVLNVAGPRASKWPGAHDYAYATMKHLLQAIAMEHR"
     gene            551..1666
                     /locus_tag="AC16CX_02110"
     CDS             551..1666
                     /locus_tag="AC16CX_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3011335.1"
                     /translation="MFLIVLISIAIHSCYIGSKVVVSLLALELGASQFLIGNIAALYA
                     LLPLLLGVYSGRLVDTIGMRVPMLIGAVLTTFAMLCGFAWQQLAALFATATIMGAAFV
                     FYNVSIQNLAGAYGRPEQRAHNFSLLTIGYSISSFIGPMIAGFSIDYAGHARAFLIFA
                     LLTLFPIAVLTFYPRLTRVQTLKSEKQERRALDLLRDPHLRRVVIMSGLMVASYELYA
                     FYTPVFGHSIGLSASTIGVIMGTYATATFLSRFFLPLVLRRMRTDQVLFSFMLLAAGA
                     FLLLPFLRNVGLLMLVSFLIGIGTGVGQPLSMTLSYDRSPAGRTGEVTGLRLTFNNIA
                     RIVIPLAAGALGTAFGAAPVFWMNALNLTAVSWLARR"
     gene            complement(1947..2234)
                     /locus_tag="AC16CX_02115"
     CDS             complement(1947..2234)
                     /locus_tag="AC16CX_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011336.1"
                     /translation="MGWKAEAHKIYEERVVNDCLARLAERLDLKGLPFSDEELKTLAR
                     RSRESFRNPEKRRERLDRYKAHLAKNYGAELVANISSALEAINNAIGYEEK"
     gene            complement(2320..>2560)
                     /locus_tag="AC16CX_02120"
     CDS             complement(2320..>2560)
                     /locus_tag="AC16CX_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011337.1"
                     /translation="TLMYVVLVTANWKTTGEIKKILPPEILLKVFAVFMVASVIFVLA
                     LLKIIQESTVSALLGALAAGVFGFTSARRGGDGDA"
BASE COUNT          468 a          839 c          738 g          515 t
ORIGIN      
        1 acgccgcgct cgacgcgggg tttccctgcg gcggctgggc gaccgaagac cgcgtcgcgg
       61 aggacggcac gatacccgcg cactatccgt tggccgagct cccgggcggc ggctacgagg
      121 agcgcactct gcagaacgtg ctcgacagcg acggcacggt gatcctccac ttcggcgtgc
      181 tggaaggcgg cacgcgccag acgcaggact gctgcaaggc gcatggcaag ccccacgaga
      241 ttatcgacgc gtccctggtt gcacccgagg aagccgcacc caggctcgcc gccttcatcg
      301 agcgtcaccg cattgctgtg ctcaacgtcg ccgggccgcg cgcgagcaaa tggccaggcg
      361 cccatgatta cgcttacgcc accatgaagc acctgttgca agctatcgca atggaacatc
      421 gctgaaccgt cacttgtcgt tcagccccct ccctcaccct ccccctcccc cttggcaagg
      481 gggagggaca gggtgggggt gaccactcga cacgcgacac ccgacactcg acactcgaca
      541 ctcctctctg atgttcctga tcgtcctgat cagcatcgcg atccacagct gctatatcgg
      601 cagcaaggtg gtggtgtcgc tgctcgcgct cgagctcggc gccagccagt tcctgatcgg
      661 aaacatcgcc gcgctctacg ccctgctgcc gctgctgctg ggggtctact ccggcaggct
      721 tgtcgacacc atcggcatgc gcgttccgat gctgatcggc gccgtcctca ccaccttcgc
      781 catgctctgc ggattcgcgt ggcagcagct cgccgcgctg ttcgcgaccg ctacgatcat
      841 gggcgcggct ttcgtgttct acaacgtctc gatccagaac ctcgcgggcg catatggccg
      901 gcccgagcag cgcgcgcaca atttcagcct gctcacaatc ggctactcga tctcgtcctt
      961 catcgggccg atgatcgccg gtttttccat cgactatgcc ggccacgcgc gcgccttcct
     1021 gatattcgca ctgctgacgc tgttcccgat cgcagtgctg actttctacc cgcggctcac
     1081 gcgagtccag acgctgaaat cggaaaagca ggaacggcgc gcactcgacc tgctgcgcga
     1141 cccgcacttg cggcgcgtcg tcatcatgag cggcctgatg gtcgcgtcat acgagctcta
     1201 cgccttctat acgcccgtgt tcgggcattc catcgggctg tcggcatcca ccatcggcgt
     1261 aatcatggga acctacgcga ccgccacctt cctctcgcgc ttcttcctgc cgctggtgtt
     1321 gcgcaggatg cgcacggacc aggtgctgtt ttccttcatg ctgctcgccg cgggcgcctt
     1381 tctgctgctg cctttcctgc gcaacgtcgg tctcctcatg ctggtttcgt tcctcatcgg
     1441 gatcgggacc ggcgtcgggc agccgctttc gatgaccttg tcctacgacc gctcgccggc
     1501 cggacgcacc ggggaagtga cgggcttgcg cctcaccttc aacaacatcg cgcgcatcgt
     1561 gataccgctc gcggcgggag cgctcggcac ggcgttcggg gccgccccgg tgttctggat
     1621 gaatgcgctg aacctgacgg cggtcagctg gctggcgcgg cgttaaagac tgaaccacca
     1681 gggacacaag ggacgctaag gaagagaaga cgggagagcg tgagggcggg agagcgggag
     1741 agggaaaata cacttcgacc tcccgatctc acggtctctc catctcacca tttctcgatt
     1801 tcacgatctc acggcttttg ggaaccgccg agaacgccaa gagaaaacga aaaattgaac
     1861 cgcggagaaa gggggagaag tttaacgtcc cggttcaggc gcgcggggct tcatgcgcgt
     1921 cgcctgcaac cgggttaggc ggcgtgtcac ttttcttcat aaccgattgc attgttgatc
     1981 gcctctagcg cagaggaaat gtttgcaaca agctcggccc cataattctt cgcgagatga
     2041 gccttgtatc tgtccaggcg ctctctcctc ttttccggat ttctaaacga ttcgcgagat
     2101 cgtctagcta aggtcttcaa ctcttcgtcc gagaatggca ggcccttcaa gtcgagtcgc
     2161 tctgccagcc gggcgaggca gtcgttcaca acgcgctcct cgtagatctt gtgtgcttcg
     2221 gccttccagc ccatgtggaa tcacgccgtc tagcgtgtcc cgatcacccg cgcgaggaag
     2281 ccagcaccgc gcgcactcgc gtcggtgtgg atcgggtggt taggcatcgc catcgccgcc
     2341 acgcctcgcg gatgtaaacc caaacacacc agctgccagg gcgccaagta gcgcgctgac
     2401 ggttgactcc tgaatgatct taagaagcgc aagcacaaag atgacactcg caaccatgaa
     2461 aacagcaaac actttgagaa gaatttctgg cggtagaatc tttttgattt cgccagttgt
     2521 tttccaattc gctgtcacta agacgacgta catcagcgtg
//
LOCUS       DBNGWZ010000079         3756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3155675, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000079 DBNGWZ010000000
VERSION     DBNGWZ010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3155675"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="AC16CX_02125"
     CDS             complement(<1..133)
                     /locus_tag="AC16CX_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3011338.1"
                     /translation="MPRRREIPKREVLPDPKYTSQDIAKFINVLMTRGKKSVAEAIVY
                     "
     gene            complement(234..611)
                     /gene="rpsL"
                     /locus_tag="AC16CX_02130"
     CDS             complement(234..611)
                     /gene="rpsL"
                     /locus_tag="AC16CX_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3011339.1"
                     /translation="MPTINQLVRKGRAPARWKSKVPALAGSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVIGYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHVVRGSL
                     DTAGVKDRKQGRSKYGAKRSKAA"
     gene            complement(826..>3756)
                     /gene="rpoC"
                     /locus_tag="AC16CX_02135"
     CDS             complement(826..>3756)
                     /gene="rpoC"
                     /locus_tag="AC16CX_02135"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3011340.1"
                     /translation="FEPVLIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSLEAQMEA
                     RTLMLSSNNILSPANGEPIIVPSQDIVLGLYYITREKVGARGEGSVFINLAEVSRAYD
                     TGQAEVNARITVRIKEVEIDADGEKREKLTRYETTVGRALLSDILPAGLPFELINKPL
                     KKKEISRLVNASFRRCGLRETVIFADKLMYAGFAMATKAGLSIAVDDMLIPSQKNEII
                     ADAEKEVQEIEKQYTSGLVTQGERYNKVVDIWGRAGDLVAKAMMEQLGVEPVMDWDAK
                     KREWTPRRDKKGAPLMQESLNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIET
                     PITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQDLVVTEDDCG
                     TVQGVSMKALIEGGEVVESLRERILGRVCAADIVNPESSLVMYPANTLLDEDAVEAIE
                     ASGIDEVKVRTPLTCDTRYGLCAKCYGRDLGRGTPVNVGEAVGVIAAQSIGEPGTQLT
                     MRTFHIGGAASRTAVVSQIESKSNGVVRYSAGMRYVTHSRGELVVISRNGEIVIHDES
                     GRERERHKVPYGATLLARDGEAVKAGHVLATWDPHTRPIITEYAGRIKFENVEEGVTV
                     MRQIDEVTGLSTLVVVDPKRRGGIQAKGLRPQVKLTDESGNEIKMAGSDQPVNITFQI
                     GSIITVRDGQEVGVGEVLARIPQETSKTRDITGGLPRVAELFEARSPKDAGVLAEITG
                     TVSFGKDTKGKQRLVITDLEGVAHEYLIPKDKHVMAHDGQVVNKGEMIVDGPADPHDI
                     LRLLGVEALARYICNEVQDVYRLQGVKINDKHIEVIVRQMLRRVQIVEPGDTRFITGE
                     QLERAEVLEENEKVEAEGKKPATYEYLLLGITKASLSTDSFISAASFQETTRVLTEAA
                     IMGKRDELRGLKENVIVGRLIPAGTGLAYHRVRRRQGAAVEFGESPPAVEEAAGAESQ
                     EQVA"
BASE COUNT          561 a         1261 c         1176 g          758 t
ORIGIN      
        1 cgtagacgat cgcctccgcg accgacttct tgccgcgggt catgaggaca ttgatgaact
       61 tggcgatgtc ctggctcgta tacttcgggt cgggcaggac ctcgcgcttc ggaatctctc
      121 tgcgtcgtgg catgttctaa accagtgtta agtgttgagt gttgagttca aacctcaggt
      181 gcgcgggctt tggggtatca ctcaacactc agcactaaaa actcaaaact ccgttatgcg
      241 gctttggacc gcttggcgcc gtacttggag cggccctgtt tgcggtcctt gacgcccgcg
      301 gtatcgagcg agccgcgcac cacgtgatag cgcacacccg gcaggtcctt cacgcgcccc
      361 ccgcgaatca gcaccaccga gtgctcctgc aggttgtgcc cttcgccgcc gatgtagccg
      421 atcacttcga agccgttggt gagccgcacc ttggccactt tgcgcagcgc ggagttcggc
      481 ttcttgggcg tcgtcgtgta aacgcgcgtg catacgccgc ggcgctgcgg cgagccggcc
      541 agcgccggca ctttgctttt ccagcgcgcc ggggcgcgcc ccttgcgcac taactggttg
      601 atggttggca tgtcctcagt aacctgcctg ctcggccgtt atcccgatta acgcgttgaa
      661 ttcacggaac ttcgggcaaa accccgcaca ctttgccggt tccaaaaaag cggggacggc
      721 gtcgccaccg tccctgcctg gattgctgat cttgtcgccg gcgtccccgt gcgggcgacc
      781 ggcgggtaaa aagactgtga attttaaggg cttacgtcac ctgagtcaag caacttgctc
      841 ctgactttcg gctcccgccg cttcctccac cgccggcggg ctctccccga attcgaccgc
      901 cgcgccctgg cgccggcgta cgcggtgata agcgagcccg gtgccggccg ggatgagccg
      961 cccgacgatc acgttctcct tcaagccgcg caactcgtcg cgcttgccca tgatcgccgc
     1021 ctcggtgagc acgcgcgtgg tttcctggaa cgaggccgcc gagataaacg agtcggtgga
     1081 gagcgacgcc ttggtgatgc cgagcagcag gtactcgtag gtcgccggct tcttgccctc
     1141 ggcctccact ttctcgtttt cctccagcac ctcggcgcgc tcgagctgct cgccggtgat
     1201 gaagcgggtg tcgcccggct cgacgatctg cacgcggcgc agcatctggc gcacgatcac
     1261 ctcgatgtgc ttgtcgttga tcttcacgcc ctgcaggcgg tagacgtcct gcacctcgtt
     1321 gcagatgtag cgcgcgagcg cctccacgcc caggagccgc aggatgtcgt gcggatcggc
     1381 cgggccgtcc acgatcattt cgcctttgtt cacgacctgc ccgtcgtgtg ccatcacgtg
     1441 cttgtccttc gggatcaggt actcgtgggc caccccctcg aggtcggtga tcaccaggcg
     1501 ctgcttgccc ttggtgtcct tgccgaagga aaccgtgccg gtaatttccg ccagcacgcc
     1561 cgcgtccttg ggagagcgcg cctcgaagag ttccgccacg cgcggcagcc ccccggtgat
     1621 gtcgcgggtc ttggaggttt cctgcgggat gcgcgcgagc acctcgccca cgcccacttc
     1681 ctggccgtcg cgcacggtga tgatcgagcc gatctggaag gtgatgttga ccggctggtc
     1741 cgagcccgcc atcttgatct cgttgccgga ctcgtcggtg agcttcacct gcgggcgcag
     1801 ccccttcgcc tgaatgccgc cgcggcgctt cgggtcaacg acgacgaggg tcgaaagccc
     1861 agtgacctcg tcgatctgcc tcatgacggt cacgccttcc tcgacgttct cgaacttgat
     1921 gcgtccggca tactcggtga tgatcgggcg cgtgtgcgga tcccaggtcg cgagcacgtg
     1981 cccggccttg accgcctcgc cgtcgcgcgc aagcagcgtc gcgccgtacg gcaccttgtg
     2041 gcgctcgcgc tcacggccgc tttcgtcgtg gatcacgatc tcgccgttgc gcgagatcac
     2101 caccagctcg ccgcgcgagt gagtgacgta gcgcatgccg gcgctgtagc gcaccacgcc
     2161 gttcgacttg ctctcgatct ggctcaccac ggcggtccgt gaggcggcac caccgatgtg
     2221 gaaggtgcgc atggtgagct gggtgcccgg ctcgccgatc gactgcgcgg caatcacgcc
     2281 gactgcctcg ccgacgttga ccggcgtgcc gcggccgagg tcgcgcccgt agcacttggc
     2341 gcacaggccg tagcgggtgt cgcaggtgag cggcgtgcgc accttgactt catcgatgcc
     2401 ggaggcttcg atcgcctcca ccgcatcttc gtcgagcagc gtgttcgcag ggtacatcac
     2461 caggctgctc tcggggttga cgatatcggc cgcgcacacg cgcccgagga tgcgctcgcg
     2521 caggctttcg accacttcgc ccccctcgat cagcgccttc atcgagacgc cctgcacggt
     2581 gccgcagtca tcctcggtca cgaccaggtc ctgggtgacg tcgacgaggc gccgcgtgag
     2641 ataacccgag ttggcggttt tcagcgcggt gtcggcaagg cccttgcgcg cgccgtgcgt
     2701 ggagatgaag tattgcagca cgttcagccc ctcgcggaag ttcgcggtaa tcggcgtttc
     2761 gatgatcgag ccgtccggtt tcgccatgag cccccgcatg ccggcgagct gccggatctg
     2821 ggctgcggaa ccgcgcgcgc cggaatcggc catcatgtag atggagttga gcgattcctg
     2881 catcaacggt gcgcccttct tgtccctgcg cggcgtccat tcgcgcttct tggcgtccca
     2941 gtccatcacc ggctcgacgc cgagctgctc catcatggcc ttcgcgacca ggtcgccggc
     3001 acggccccag atgtccacca ccttgttgta gcgctcgccc tgggtcacca ggccggaggt
     3061 gtactgcttc tcgatttcct gcacttcttt ctcggcgtcc gcgatgatct cgttcttctg
     3121 cgacgggatc agcatgtcgt ccaccgcgat ggaaaggccc gctttggtgg ccatggcaaa
     3181 gccggcgtac atcagcttgt cggcgaagat caccgtctcg cgcaggccgc agcgacggaa
     3241 gcttgcattg acgagacggg agatctcctt cttcttgagt ggcttattga tgagctcgaa
     3301 gggcaatccc gccggcagga tgtcggagag cagcgcgcgc ccgacggtgg tttcgtagcg
     3361 cgtcagcttc tcgcgcttct cgccgtcggc gtcgatctct acttccttga tgcgcaccgt
     3421 aatgcgggcg ttcacttcgg cctggcccgt gtcgtacgca cgcgacacct cggcgagatt
     3481 gatgaagacc gagccttcgc cgcgcgcgcc caccttctcc cgggtgatgt agtagagccc
     3541 gagcacgatg tcctgcgaag gcacgatgat cggctcgccg ttggcgggcg acaggatgtt
     3601 gttggaggaa agcatgaggg tcctcgcctc catctgcgcc tccagcgaca gcggcacgtg
     3661 caccgccatc tggtcgccgt cgaagtcggc gttgaacgcg gcgcacacca gcgggtgcag
     3721 ctggatcgcc ttgccctcga tcagcaccgg ctcgaa
//
LOCUS       DBNGWZ010000080         7727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1896367, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000080 DBNGWZ010000000
VERSION     DBNGWZ010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7727
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1896367"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16CX_02140"
     CDS             <1..464
                     /locus_tag="AC16CX_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3011341.1"
                     /translation="MFIFYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQSWKYTMP
                     AFLVPFVFVLDPAGLGLLLMGSFKGLAGASVVDIAVVSVTAMMGIAALAGGMQGWVFK
                     KTNSLERWLLILAGLFLVYPRAAFDYIGLALLIAVVMMQKLRKGEVHAAAG"
     gene            448..927
                     /locus_tag="AC16CX_02145"
     CDS             448..927
                     /locus_tag="AC16CX_02145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3011342.1"
                     /translation="MLRLDSYQAKFPAPFAVLGIRTAGESLTDIEYLPRGVATLPPLN
                     RLAERVCNQLERYLDDPEFRFDLPFDYRGTEFQCRVWRAISGIPSGRTLSYVDIARRL
                     RTAPRPVGGACGANRIPIVIPCHRVVGVSGIGGFMNARSGAPLEIKRWLLTHEGAKH"
     gene            complement(1126..2109)
                     /locus_tag="AC16CX_02150"
     CDS             complement(1126..2109)
                     /locus_tag="AC16CX_02150"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3011343.1"
                     /translation="MKLHNLSWRVFSAGHMGTLAKAVTILLPCFFLLQAPMRVSAQTI
                     PIIDMHFHADQVWDVQALVKLFDELGVAAAGNGARGSDGLVLKFAQQYPGRLIPYGGN
                     EPIRGFISREGARAYSLESPAVVEYLGQLEAALRDKQFKGIGELVVNSTHSRADKASK
                     YPADSPLMRRLWALSAKYGVPMSAHMDATPESIQQMERLLASDRRGTWVWAHTGWLPV
                     ADPSMLRRLIQAHPNLFCELSGRESIRQKYRGDPIDEGGVLKPTWKALLEEFPDRFVI
                     GTDVDPATLKTYAEEIGWWRGILTQLSPTTAAKLAHENAERILKLPAVASR"
     gene            2356..4071
                     /locus_tag="AC16CX_02155"
     CDS             2356..4071
                     /locus_tag="AC16CX_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HIR3011344.1"
                     /translation="MPVGTESPAAPGKERLQVGDWAVEPALNQLSAAGKTVKLEPKAM
                     AVLVYLADRPGQVVSREALLSAVWSGVVVGDDSLTQVVIKLRKALGDAPEEPAYIQTI
                     SKGGYRLVAPVVRSAEISSAPVRPDSDPLYAGRKRRVPWIAGAGIVVLLFAAAGVWWI
                     KGERVTGDPLGQAPMASTEAARTAQPTVAIKPFEALGNDPQEVLLAQAITADLVTDLS
                     KVFGLWVIGVAPTGGHAGGEAPTDAPPIHYVVSGSVQRVDERLRLHVHLTDAETGKQL
                     WSERFDREMNGLFAIQEELGPKILRMLPAKVSEAEMRRVAQRHTRNLEAYEYFQRGQS
                     ALLVRQRAENETAREMFWRAIKLDAAFARAYAGLALTYAADYRNQWTGDGAAALDRAS
                     EMARTAHQINPDIPETYWVLAYVHEQRRQHERALQYLETAVRLYPSFADGHALMGSVN
                     THVGRPADTIPLLRTAMRLNPQAGYLYFLVLGRAYLFLGDLEQARVNLEHALSRNLVN
                     LEAHVYMAAVHVTAGDKAAAAWEAEEIRTLQPGFSSRRWLETYPMTDAAQKTKLIRTL
                     GELGF"
     gene            4315..5205
                     /gene="xerD"
                     /locus_tag="AC16CX_02160"
     CDS             4315..5205
                     /gene="xerD"
                     /locus_tag="AC16CX_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR3011345.1"
                     /translation="MGSSLIDEFCDALWLEDGLARNTLEAYRRDLAQLAQWLKQVHGK
                     PLAAADHADLLGYLAYRVRGRARATTTGRQLSSLRRFYRYCLRQGEIKADPTLNIDSP
                     RLPRSLPKSLTEEDVENLLAAPPVEEALGLRDKAMLETLYASGLRVSELVALKLGQVS
                     QDMGVVRVVGKGSKERLVPLGEEALAWIQRYLKEARPGLLGGRASDDLFVTARGAAMT
                     RQMFWQLLRRYARKAALSKPISPHTLRHAFATHLLNHGADLRVVQLLLGHSDISTTQI
                     YTHVARERLKQLHAKHHPRG"
     gene            complement(5278..5634)
                     /locus_tag="AC16CX_02165"
     CDS             complement(5278..5634)
                     /locus_tag="AC16CX_02165"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR3011346.1"
                     /translation="MDVIFITDLRLDILIGIYDWERKVPQTVQFDLEIGLPDTGRPRS
                     DKIGETIDYSKIVARICSSVKSERFILVERLAEHIAQLVMTEFRSPWVKVSVTKLAAL
                     KDVKRVGITIERGKKQ"
     gene            5787..6416
                     /gene="plsY"
                     /locus_tag="AC16CX_02170"
     CDS             5787..6416
                     /gene="plsY"
                     /locus_tag="AC16CX_02170"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3011347.1"
                     /translation="MTLVALTLLAYLLGSIPFAVIVSRAFALPDPRSYGSGNPGATNV
                     LRTGRKAAAALTLLGDAAKGWLAVLIGGRFADAGSVELTVAAAAVAVALGHMYPLFLR
                     FHGGKGVSTSLGVLIALDPWLAAGAAATWIIIAFFFRISSLAALVAAAFAPFFSFFLY
                     GMNRFALEPAVGVTVISLLLVWRHRSNIRNLIAGTEGRIGGGSTGSPQS"
     gene            complement(6413..7441)
                     /gene="tsaD"
                     /locus_tag="AC16CX_02175"
     CDS             complement(6413..7441)
                     /gene="tsaD"
                     /locus_tag="AC16CX_02175"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3011348.1"
                     /translation="MLVFGIETSCDETGVALYDGRRGLLGHALYSQARLHADYGGVVP
                     ELASRDHVRRLLPLTRGLLEQTGCRLDMLTAVAYTEGPGLAGALLVGAAIAHGLAHAL
                     GIPALGVHHLEAHLLSPLLSRPAPAFPFVALLVSGGHTQLMRVAGVGDYELLGETLDD
                     AAGEAFDKTAKLLGLGYPGGPALARLAGHGTPGRFSLPRPMIASGDLDFSFSGLKTAV
                     LTAVKARVLDESARADLAAEIEAAIVEVLAAKALAAVVYAGLKRLVVAGGVGANLSLR
                     ARLVAGARARGVEVFYPELEFCTDNGAMIAFAAALRMQRSAVSRQPPALSFTVRPRWD
                     LETVAAQG"
     gene            7515..7727
                     /gene="rpsU"
                     /locus_tag="AC16CX_02180"
     CDS             7515..7727
                     /gene="rpsU"
                     /locus_tag="AC16CX_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3011349.1"
                     /translation="MPTVRVKENEPFEAALRRFKRTVEKTGLLTELRAREYYEKPTTA
                     RKRKLAAAIKRHHKKLRAQLLPPRMY"
BASE COUNT         1403 a         2459 c         2487 g         1378 t
ORIGIN      
        1 acatgttcat cttctactac gcggtgctgt cggaggtctc gccgcccacc gcgctctcgc
       61 cgttcgcggc ggccgcgatc accggtggcg acccgtacaa gacgacgctg cagtcgtgga
      121 agtacacgat gccggcgttc ctggtgccgt tcgtgttcgt gctcgaccct gcgggcctgg
      181 ggctgctgct gatgggctcg ttcaagggcc tggcgggcgc ttcggtggtg gacatcgcgg
      241 tcgtgagcgt gacggcgatg atgggcatcg cggcgctcgc cggcggcatg cagggctggg
      301 tgttcaagaa gacgaacagc ctcgagcggt ggctgctcat cctcgcggga ttatttctgg
      361 tttacccgcg cgcggcattc gactatattg ggctcgcgct cctcatcgca gtggtcatga
      421 tgcagaagct gcgcaagggc gaggtccatg ctgcggctgg atagctacca ggcgaaattt
      481 cccgcgccgt ttgcggtgct cggcatccgc accgccggag aaagcctgac cgatatcgaa
      541 tatctgccgc gcggggtcgc gacgctcccg cccctcaacc ggctcgccga gcgggtgtgc
      601 aaccagctcg agcgctacct cgacgatccc gaattccgct tcgacctgcc gttcgactat
      661 cgcggcaccg agttccagtg ccgggtgtgg cgcgcgattt ccggcatacc cagcggcagg
      721 accctcagct acgtggatat cgcgcgccgg ctccgcacgg cgccgcgtcc cgtcgggggc
      781 gcgtgcggcg ccaaccgcat tccgatcgtg attccctgtc accgcgtggt gggcgtgagt
      841 ggtatcggcg gtttcatgaa cgcgcgaagc ggggcgccgc tcgaaatcaa gcggtggctt
      901 ctcacgcatg agggagcaaa acactagaag agaactgaat gggtcacgta gtcgcgtccc
      961 gtagtgagtg cggagttcgc gagcaggctg aacgcgtgca tgacagcacg ccccaacagc
     1021 atggtgcctt ttgagggctg gacggcgaaa ctaatcggcg agcctaactt ctcagcagcg
     1081 gcaagagagg ctacccagga gccgaaccag tatccaggcc gatagttagc gactcgctac
     1141 ggctggaagt ttcaagatgc gctcggcatt ctcatgggcc agcttcgctg ccgtcgtcgg
     1201 cgacagttgc gtaaggatgc cgcgccacca tccaatttcc tccgcgtacg tcttcagggt
     1261 ggccggatcg acgtccgtcc cgatgacaaa ccgatccggg aattcttcaa ggagcgcctt
     1321 ccaggttggc ttaagcacgc cgccctcgtc gatcggatcg ccccggtatt tttgacgtat
     1381 gctctcccgc cctgagagct cgcaaaacag gttcggatgt gcctgaatca gccgtcgcaa
     1441 catggaggga tccgcaacgg gaagccaccc cgtgtgagcc cagacccagg tgccccgacg
     1501 gtccgaggcc agtagccgct ccatctgctg aatcgactcg ggggtggcgt ccatatgagc
     1561 gctcatgggg acgccgtatt tcgcggaaag cgcccacagc cgccgcatca acggagaatc
     1621 ggctggatac ttcgacgctt tgtctgcgcg actgtgggtg ctgttcacca ccagctctcc
     1681 gatgcccttg aattgcttgt cgcgcaatgc agcttctaac tgcccgagat attcgacgac
     1741 tgccgggctc tccaggctgt aggcccgcgc cccttcgcgg cttatgaacc cccggatcgg
     1801 ttcgttcccg ccatagggaa ttaaccgacc gggatactgc tgcgcgaact tcaggacgag
     1861 gccatccgag cctctcgcac cgttgcctgc cgcggccaca cccaattcgt cgaacagttt
     1921 caccagggcc tgaacgtccc acacctgatc cgcgtggaag tgcatatcga taatcggaat
     1981 ggtctgggct gagaccctca ttggggcctg caagaggaaa aagcaaggaa gaagtatcgt
     2041 taccgctttc gcaagtgttc ccatgtgccc agcgctgaac acccgccaag acagattgtg
     2101 aagtttcatg ctcacctcca tagaaatcat attaaaacgg aaaaacagca gagcagttcg
     2161 gaatccttgt cgttcatcgg ggcataaagc atagcctccc gcaatccgct agccagcggc
     2221 ccaacgccgg aattgccggc agcattgaac gcgggcttgg gaagaaagtc ccgaagaaac
     2281 gactaagctt tggcgaagga tgcgaaacgt cgcataattc agcacgttgg actgaggtca
     2341 ccttgaggcc gaatcatgcc cgttggcact gaatccccag ccgcgccggg caaggagagg
     2401 ctgcaggtcg gcgactgggc ggtcgagccc gcgctgaatc aactctcggc cgcaggcaag
     2461 accgtgaagc tcgagcccaa ggccatggcg gttctcgtct accttgcgga ccggccgggg
     2521 caggtcgtga gtcgggaagc gctgctttca gccgtttggt ccggtgtggt tgtgggcgac
     2581 gactccctca cccaggtcgt catcaagttg cggaaagcac tgggggatgc gccggaggag
     2641 ccggcttata tccagacgat ctcgaaggga ggctaccggc tggtcgcccc agtggtccgg
     2701 tcggcggaga tctcctccgc accggttcgg ccggattcgg accccttgta cgccggacgc
     2761 aaacggcgcg tcccatggat agcgggagcg ggcatagtcg tactactgtt cgctgcagcc
     2821 ggagtctggt ggatcaaggg cgaacgcgta accggcgacc cgctgggtca ggcaccgatg
     2881 gcaagcaccg aagcggcccg cacagcgcaa ccgaccgttg ccattaagcc gttcgaggca
     2941 ctgggcaacg acccgcagga ggtgctgctg gctcaggcca tcacagcgga cctcgtgacc
     3001 gacctgtcga aggtgttcgg gctctgggtc atcggcgttg cgcctacggg tggccatgcg
     3061 ggcggcgagg cgccgaccga cgctccgccg atccactatg ttgtgtcggg gtccgtccag
     3121 cgcgtcgacg agcgcctgcg gttgcacgtc catctcacgg acgcggagac gggaaagcag
     3181 ttgtggtccg agcgtttcga ccgcgagatg aacggcctct tcgcgatcca ggaagagctt
     3241 gggccgaaga ttctccgtat gctgcctgcg aaggtaagcg aggccgaaat gcggcgtgtt
     3301 gcgcagcgcc acacccgcaa tctggaagcc tacgaatact ttcagcgcgg gcagtcggcg
     3361 ctgctcgtcc ggcaaagagc ggagaacgag accgcacgcg aaatgttctg gcgcgccatc
     3421 aagctggacg cagcctttgc tcgtgcgtat gcaggactcg cactgactta cgccgcggac
     3481 tatcgtaatc agtggaccgg agacggcgcc gccgccctcg accgcgcatc cgagatggcc
     3541 cggactgcac accagatcaa cccggacatt ccggagactt actgggtgct ggcctacgta
     3601 catgagcagc ggcgccagca cgagcgggcg ctgcagtact tggagaccgc cgtgcggctc
     3661 tatccatcgt tcgctgatgg ccatgcgctg atgggcagcg tcaacacgca cgttgggcgt
     3721 ccggcggata ccataccgtt gttgcgcacc gcaatgcgcc tcaaccccca ggcgggctat
     3781 ctctactttt tggtgctcgg acgggcctat ctgtttctcg gcgatctgga acaggcgcgg
     3841 gtcaatctgg agcacgcgct gtcacgtaac cttgtgaacc tcgaggcgca tgtctacatg
     3901 gcggcggtgc acgtaacggc tggcgacaaa gcggctgcgg cttgggaagc ggaggaaatc
     3961 cgcacgcttc aaccgggctt ttccagccgt cgttggctgg aaacctatcc catgaccgac
     4021 gccgcgcaga aaacaaagct catccggacg ctcggcgagc ttgggttctg agaccgcgac
     4081 catcgcacct cgccggcgaa ctccgtgcag tgggtgaaga tcgtcaagcg actcaagtaa
     4141 gcgcggaaaa gcggtaaacg gtgaacagtg aacgatgagc agtgaacgca gtcaatagat
     4201 tcgggttttc cactatgacc aatcgttttt ccttcgtgtc cttggcggtg aaagcttttg
     4261 gggttatcgg cgtttatcgg cgtgcatcgg cggctaatgt tttcgttttt tgagatgggt
     4321 tctagcctga tcgacgaatt ctgcgacgcg ctgtggctcg aggacgggct cgcgcgcaac
     4381 acgctcgaag cctaccgccg tgatctcgcg cagctcgcgc agtggctgaa gcaggtgcac
     4441 ggcaagccgc tcgccgccgc cgaccacgcc gacctgctcg gctacctcgc ctacagggtc
     4501 aggggccgcg ccagggcaac cactaccggc cgccagctct cgagcttgcg gcgcttctac
     4561 cgctactgcc tgcggcaggg ggagatcaag gccgacccga cgctgaacat cgattcgccc
     4621 aggctaccgc gttccctgcc caagagcctg accgaggaag acgtggagaa cctccttgcc
     4681 gcgccgccgg tggaggaggc gctcgggctg cgcgacaagg cgatgctcga gacgctctac
     4741 gcgagcgggc tgcgcgtctc ggaactggtg gcgctcaagc tcggccaggt gagccaggac
     4801 atgggcgtcg tgcgcgtcgt cggcaagggt tcgaaggagc gcctggttcc gctgggcgag
     4861 gaggcgctcg cctggatcca gcgctacctc aaggaagcgc ggcccggact gctcggcggg
     4921 cgcgcgagcg atgatttgtt cgtcaccgcg cgcggggcag cgatgacgcg gcagatgttc
     4981 tggcagttgc tgcgccgcta cgcgcggaag gcggcgttga gcaagcccat ttcaccgcac
     5041 acgctgcgcc acgccttcgc cacgcacctc ctgaaccacg gcgcggatct gcgcgtcgtg
     5101 cagctcctgc tcgggcattc cgacatttcg accacgcaaa tctacacgca cgtggcgcgc
     5161 gagcggctga agcagctgca cgccaagcat cacccgcgcg ggtgatgttt gagggatggg
     5221 ggatgaagga tgagggatga cccccgcatt gcggaaagac tcatcaccca tcacccatca
     5281 ctgcttcttg ccgcgctcga tggtgatgcc gacacgcttg acgtccttta gggccgcgag
     5341 tttggtgacg ctgactttca cccacggcga cctgaattcc gtcatcacga gctgcgcgat
     5401 gtgctcggcg agacgctcca ccaagataaa gcgctcgctt ttgaccgacg agcagatgcg
     5461 cgcgacgatc ttactgtaat cgatcgtctc gccgatcttg tcgctgcgcg gccgaccggt
     5521 atccggcagg ccgatctcga ggtcgaattg caccgtctgc ggcaccttgc gctcccagtc
     5581 gtagatgccg atcaggatgt cgaggcgcag gtccgtgatg aagataacgt ccattcggag
     5641 cgggtgacga gtgtcgggtg tcgggtgtcg agtgtcgagt gacaaagtag cggggacaca
     5701 tagcgcccgc aaatctctcg accctcgacc ctcattcctt gttgggcagg tcggatagaa
     5761 tgggcgctca ttgtaagtga attcgcatga ccctggttgc gctgacgctg cttgcctatt
     5821 tgctcggctc gatcccgttc gcggtgatcg tgagccgcgc gttcgcgctg cccgacccgc
     5881 gcagctacgg ctcgggcaat cccggcgcga ccaacgtgct gcgcaccgga cggaaggccg
     5941 ccgccgcgct cacgctgctc ggcgatgcgg caaaaggctg gctcgcggtt ttgatcggag
     6001 gccgcttcgc cgacgccggc tcggtcgagc tcaccgtcgc ggccgcggcg gtggcggtgg
     6061 cgctcgggca catgtacccg ttattcctgc gcttccacgg cggcaagggc gtctcgacct
     6121 ccctcggcgt gctcatcgcg ctcgatccgt ggcttgccgc cggggcggcg gcgacgtgga
     6181 tcatcattgc ttttttcttc cgcatctcgt cgctggcggc gctggtcgcg gcggcattcg
     6241 cgccgttctt ctcgttcttc ctttacggca tgaaccgctt tgcgctggag cccgccgtcg
     6301 gcgtgaccgt catatcgttg ctgctggtat ggcgccaccg cagcaacatc cgcaatctca
     6361 tcgccggcac cgagggcagg atcggcggcg gcagtaccgg ctcgccgcag agctagcctt
     6421 gggccgcgac ggtctcgagg tcccaccgcg gccgcaccgt gaagctcaag gctggcggct
     6481 gacggctgac ggctgaccgc tgcattcgca gcgccgctgc gaatgcaatc atggcgccgt
     6541 tatcggtaca gaactccagc tccgggtaga aaacctcgac accccgcgcc cgcgcgccgg
     6601 cgacgagccg ggcacgcagg ctcagattgg cgccgacacc gcccgcgaca acgagtcgct
     6661 tgaggccggc gtatacgacg gcggccagcg ccttggcggc gagcacttcc acgatcgccg
     6721 cctcgatctc cgcggcgaga tccgcgcgcg ctgactcgtc gagcacgcgc gccttcaccg
     6781 ccgtcagcac cgcggtcttc aatccgctga agctgaagtc gagatcgccg ctcgcgatca
     6841 tcggccgcgg caggctgaaa cggcccggcg tgccgtgccc ggcgagcctg gcaagcgcgg
     6901 gaccgcccgg atagcccagg cccagcagct tggccgtctt gtcgaaagcc tcgcccgccg
     6961 cgtcgtcgag cgtttctccc agaagctcgt aatcgcccac cccggcgacg cgcatcaact
     7021 gggtatggcc gcctgagacg agcagggcga cgaacggaaa ggccggcgcc ggcctggaaa
     7081 gcagcggcga gagcaggtgc gcttcgagat ggtgcacgcc cagcgctgga atgccgagcg
     7141 cgtgggcgag accatgcgcg atcgcggcgc cgaccagcag cgcgccggcc agccccgggc
     7201 cttccgtgta ggcaacggcg gtgagcatgt ccaggcggca tccggtttgt tcgagcaggc
     7261 cgcgggtcag aggcagcagg cgccgcacgt gatcgcgcga ggcgagctcc ggcaccacgc
     7321 ccccgtagtc ggcatgcagc cgcgcttgcg aataaagggc gtgtccaagc aggccgcggc
     7381 ggccgtcata gagcgcaacg ccggtctcgt cgcaggaagt ttcgataccg aacaccagca
     7441 tgggggatag cgcgccttca aaaaggcctt atagcttgat ataatacaag ttttccaatt
     7501 caagccagag atctatgccg actgtcagag tgaaagagaa tgagccgttc gaggcggcgc
     7561 tacgccgctt caagcgcacc gtggaaaaaa ccggcctgtt gaccgaattg cgcgcccgcg
     7621 agtactacga aaaacccacc accgcgcgca agcggaagct cgccgccgcg atcaagcgcc
     7681 atcacaagaa gctgcgcgcc cagctgctcc cgccgcggat gtattga
//
LOCUS       DBNGWZ010000081         9210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1015179, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000081 DBNGWZ010000000
VERSION     DBNGWZ010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1015179"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..3005
                     /locus_tag="AC16CX_02185"
     CDS             429..3005
                     /locus_tag="AC16CX_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HIR3011350.1"
                     /translation="MKKLDFWRSDWFLGVVVAVLLLVFSGGDLIQSLERKAYDLAVQA
                     TSRVPSDRIAVIAIDDTSIANIGRWPWSREVLAKMTDLLAGAKAKVIGSTILLDQPQI
                     DPGYQYITKLLEIAGKRTLAPQPGVDPAAPAPQPMGEMEQFVALLREAEQALNTDRKL
                     AESYAKAGNVLQPMLFALGEPRGKPDKPLPDYVQKNALPGIKVAGGDLPLPTAAVQVP
                     IDVIGTKAVALGHLNANPDVDGGVRTEPLVLQYFDQFYPSLSLMIAARSLNLGVNDIK
                     VKLGESVSLGKLNIGTDPALQMHTYFYKDRGDKPAFPIDSFYDVISGKIPATKYQDKI
                     VLIGATAAGLGATQVTPISPATPPVLTMAHSVSSILGEHFFVAPTWGLWVEKLVFLLV
                     ALYLILLLPRLKAGMAAMLTGAIFIVLVAAHFVLMVTQLMWIQLMAAVTLLLIGHLAL
                     TTKRYLVTERGKEKSDIESAQSNRMLGLAYQGQGQLDTAFDYFRKLPLDDGIMDPLYN
                     LALDFERKRQFNKAESVFRYMADYNLKFRDLESRLSRAKAMSETVILGAGGSHPGGTV
                     ILPGGGVEKPMLGRYQVEKELGKGAMGIVYQGKDPKIGRVVAIKTMALSQEFEADELK
                     DVKERFFREAETAGRLTHPNIVTIYDAGEEHDLAYIAMEFLKGKDLTPFVKPENLLPP
                     DKVLSIIARVAEALAYAHKNNVVHRDVKPANIMYEPESDSVKVTDFGIARITDSSKTK
                     TGMVLGTPSYMSPEQLAGKKIEGRSDLFSLGVTLYQLLCGHLPFQGDSMAQLMFKIAN
                     ETPTDILTYNPKLPPSVIEIIDKALAKQPEDRYQSGEEMAQAVRLCDAAPTKPGAMDV
                     SL"
     gene            3014..3829
                     /locus_tag="AC16CX_02190"
     CDS             3014..3829
                     /locus_tag="AC16CX_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR3011351.1"
                     /translation="MINLSSALEVATATHSGMVRSHNEDSIAADQGIGLAVLADGMGG
                     YNAGEVASGIAVELIKAEMAKALAGKKPEELNGQSAERLIAEHATRANAAIYQAAQSQ
                     PQYSGMGTTLVVALWHNNQVSVGHVGDSRLYRLRGGTLEQITRDHSLLQEQIDSGMIT
                     KEQARYSQNKNLVTRAVGIDPEVETEVHTYPAQPGDIYLLCSDGLSDMVTDEDIQLAL
                     ASLQSNLPLAAQQLVQQANDNGGRDNVSVILVRVVKSFQARTGWLEKLKAWFR"
     gene            3847..4692
                     /locus_tag="AC16CX_02195"
     CDS             3847..4692
                     /locus_tag="AC16CX_02195"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3011352.1"
                     /translation="MAKLILSLEGSVIREVPLNKERVTIGRKPQNDIQIENLAVSGEH
                     ACIVTILNDSFLEDLGSTNGTLVNGNPIKKHILQNNDVIEIGKYKMKYVVEAAASEHA
                     PPEDFEKTMVMRAPPKAATPAKADAAPAAAPKPAAAPAATPPLAPKAVTPAAAPAPAP
                     KPAAPAAAAAPAPRPAAPAAAPGAAPKPAAVESQPLAALQILTGAAAGREIELTKNLT
                     TIGKPGLQVAVITRRPQGYYITHVEGANLPVVNGKKLDAQAHQLNDHDVIELAGVKME
                     FFVKR"
     gene            4871..7132
                     /locus_tag="AC16CX_02200"
     CDS             4871..7132
                     /locus_tag="AC16CX_02200"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3011353.1"
                     /translation="MLATIKKHLARIIIGLVVVLVFLVHTVGLWGLRLPLLENLEAII
                     YDTRLRLTMPGGVDPRIVILDIDEKSLAEKEKGGEGRWPWPRDRLASLLDVLFDKYGI
                     AVAGFDVVFAERDESSGIRVLERLGARELHDVPQFQSVLKQIKPELEYDDIFARKMKG
                     RAVVLGHIFSSDEGGAAPKKGALPAPIFSAKSFAGRTIRTTSWQGYTANLEVLQKNAA
                     GAGHFNSNTDPDGIVRRVPMLAEYDGGYYEPLSLTIVRVLLGFPPVKALSPETTALAR
                     KGYPDLEWLDVGSLRIPVDDTASALIPYRGRKGSFKYFSLVDVLNERVDVAELKGKIV
                     IVGTTAPGLLDLRATPVDSVYPGVEVHANMIAGMLDGHIKQRPPYVLGAEFMLLLFTG
                     LMMALLLPLLNPLRSTLVTLLVLVAVLAINVMVFQYGHLVLPLASGITMVLLLFTLNM
                     AYGFFIEARGMRQITGLFGQYVPPELVERMADNPERFNMAPRAEELSVLFSDVRGFTT
                     ISESLTPEDLATYINEYLTTMSLVIRERHHGTLDKYIGDAIMAFWGAPVADPRHAMNA
                     VQAAIDMQSEAKILNEKFKAKGWPTFKIGIGVNSGVMRVGDMGSKIRKAYTVMGDAVN
                     LGSRLEGITKQYGADIIIGEGTKAAISGIVCREIDRVRVKGKDEPVAIFQPLGLEGQV
                     GRSKLDEIKLWNQALKYYRAQQWDQAELQLLNLKKSAPDGALYDEFVERIGFLRAHPP
                     GADWDGVWKFETK"
     gene            7132..7896
                     /locus_tag="AC16CX_02205"
     CDS             7132..7896
                     /locus_tag="AC16CX_02205"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HIR3011354.1"
                     /translation="MKLRILGCSGGIGGDLRTTSMLLDHDTLIDAGTGVGDLSINELA
                     QIDHVFVTHSHMDHVASIPFIVDTVGWMRDKPLTLHATRETVAILKDHLFNWKLWPDF
                     TQIPDAKNPVLRYETVTVGSGAALNGRRITPLPANHVVPAVGYHLDSGKASLVFTGDT
                     TSNDALWPIVNEISNLKYLIIETALSDREQELARMSKHLCPSLLAEELAKLKLAPEIY
                     ITHLKPRESEIIMQQIAVKVKERRPRMLQNNHVFEF"
     gene            7985..>9210
                     /locus_tag="AC16CX_02210"
     CDS             7985..>9210
                     /locus_tag="AC16CX_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3011355.1"
                     /translation="MSTVLQSKPVSPENLAERLQFQQKLQAVTNKVHATKNIDEIILE
                     LSQELCNLFNADRLTIYLTSEDKGSIISKVKTGLNSFKDIKLPINEQSIAGFVAGNKR
                     VVNIRDVYDDAELKSYSQQLNFLKAVDAKTGYRTKQMLVAPVLEAKTKDLIGVVQIIN
                     AKTGHPFPPIMEEGVVQLGETLAIAFRQRQGPMMGVRTKYDGLVTNAVISAEELELAQ
                     RSARRKNLDLETILIDEFKVNVPAMGSALSGYFGVPYEPYRQDRIKPPDLLKNMSREF
                     CQTNQWVPLEDAKEGLIVMAIDPEKTKASKQVNNVYPKGKIVFRVTTNREFASTLDQM
                     FGGGGVGALADSGDIGDILGTLDDESAVVEGAPEDVASAANDNELVKLVNKIIIDAYQ
                     QGASDIHIEPYPGKAKT"
BASE COUNT         1963 a         2762 c         2857 g         1628 t
ORIGIN      
        1 acggtccagt ccatgaccga tgtgctcatc gccgagttcg acaacgccgc gcttgggcgt
       61 atgagcgagg gttgtcagct ccaatttgcg cgtggactgt tgcgcacgct ggtcgagcgc
      121 ctcgctctgg cgaacgtccg gatagcccag tcgggataag atctcacaac cccggacacc
      181 tcccgaatcc ggacgcccaa cggcaccgct tcctttcctg cagtattcag atcgtccgcc
      241 gtcggttacg gcgcctcgtt atctggatat cagtcctgct gccgcggttt cgctgaccgt
      301 cgtaattccc tgtatttaca gaaagttttg gagtccgttt gccaaaaaaa catatacttt
      361 tccggcgaat atcgtagaat gaccagtcgg tagcgttgac ccgcaaaaaa acaacaggag
      421 gggcagggat gaaaaagctc gatttttggc ggtccgactg gttcctcggg gtcgtggtcg
      481 cagtgctatt gcttgttttt tcaggcggtg acctgatcca gagcttggag cgcaaggcct
      541 acgatctggc tgtgcaggcc acatcccgtg ttccctcgga taggatcgcg gtgatcgcga
      601 tcgacgacac ctcgatcgcc aacatcgggc gctggccgtg gtcgcgcgag gttctcgcca
      661 agatgaccga tctgctcgcc ggcgccaagg cgaaggtgat cggcagtacg attcttctcg
      721 accaacctca gatcgatcca ggctaccagt acattaccaa gctgctggaa atcgccggca
      781 agaggacgct tgcgccgcaa ccgggcgtgg atccggcggc gcccgcaccg cagccgatgg
      841 gggagatgga gcaattcgtc gcgctgctca gggaggcgga gcaggctctg aataccgacc
      901 gcaagctcgc cgaaagctat gcgaaagccg gtaatgttct gcaaccaatg ctgttcgcgc
      961 tgggcgagcc gcgcggcaag cccgacaagc cgctgcccga ttacgttcag aagaacgctc
     1021 ttccgggcat caaggtggcg ggaggcgacc tgccgctgcc gacagccgcc gtgcaggtgc
     1081 cgatcgacgt catagggacg aaggccgtgg cgctgggcca cctcaatgcc aaccccgacg
     1141 ttgacggtgg cgttcgtacc gaaccgctgg tgctgcagta cttcgaccag ttctatccgt
     1201 cgctgtcgct gatgatcgcg gcgagaagcc tcaacctcgg cgtgaatgac atcaaggtga
     1261 agcttggcga aagcgtgtcg ctaggcaagc tcaacatcgg caccgaccca gcgctgcaaa
     1321 tgcacaccta tttctacaag gaccggggcg acaagccggc gtttccgatc gactcgttct
     1381 acgacgtgat cagtggcaag atccccgcta ccaagtacca ggacaagatc gtcctgatag
     1441 gggctaccgc cgctggtctc ggcgccacgc aggtgacgcc gatctcgccg gcgacccccc
     1501 cggtgcttac catggctcat tcggtttcca gcattctcgg cgagcatttc ttcgtcgcgc
     1561 caacgtgggg cctgtgggtc gaaaagctgg tattcctgct ggttgcgctt tatctgatcc
     1621 tgctgctgcc gcggctcaag gccggtatgg cggcgatgct caccggagcc attttcatcg
     1681 tgctcgtcgc ggcgcacttt gtgctgatgg tgacacagct catgtggatc cagctgatgg
     1741 cagcagtgac gctgttgctg atcgggcacc tcgcgctcac caccaagcgt tacctggtca
     1801 ccgagcgcgg caaggagaag tccgatatcg agtcagcgca aagcaaccgc atgctggggc
     1861 tcgcctacca ggggcaggga cagcttgaca ccgcgttcga ctattttcga aagctgccgc
     1921 tcgacgacgg tatcatggac ccgctctaca acctcgcgct cgatttcgaa cgcaaacgcc
     1981 agttcaacaa agcggaatcg gtttttcgct atatggcgga ttacaacctg aaattccgtg
     2041 accttgagag ccgcttaagc cgcgccaagg cgatgtcgga aacggtcatt ctcggcgccg
     2101 gcggcagcca tccgggtggc accgtgatcc tgccgggcgg tggagtggaa aagcccatgc
     2161 tcggccgcta ccaagtggaa aaagaactcg gcaagggagc gatgggcatc gtctatcagg
     2221 gaaaggaccc gaagatcggg cgcgtggtgg cgatcaagac catggcgctg tcgcaggagt
     2281 tcgaggccga cgagctgaag gacgtcaagg agcgtttttt ccgcgaagcc gaaaccgcgg
     2341 gtcgcctcac ccatcccaac atcgtcacca tttacgatgc cggagaggag cacgatctcg
     2401 cctatatcgc gatggaattc ctcaagggca aggacctgac gccgttcgtc aagcccgaga
     2461 acctgctgcc gccagacaag gtgctttcca tcatcgcgcg cgttgcggaa gcgctcgcct
     2521 acgcacacaa gaacaacgtc gtgcaccgcg acgtcaagcc cgctaacatc atgtacgaac
     2581 cggaatccga cagcgtcaaa gtgaccgact tcggcatcgc gcgcatcacc gattcgtcca
     2641 agaccaagac cggcatggtg ctcggcaccc cttcctacat gtcgcccgaa caactcgccg
     2701 gcaagaagat cgaggggcgt tcggacctgt tttccctcgg cgtcacactg tatcagctgc
     2761 tgtgcggaca tttgccgttt caaggcgatt cgatggcgca gcttatgttc aagatcgcga
     2821 acgagacgcc taccgacatc ctcacttaca acccgaagct gccaccgtcc gtgatcgaga
     2881 tcatagacaa agcgctcgcc aagcagccgg aagaccgata tcagagcgga gaggagatgg
     2941 cacaggccgt ccgcttatgc gacgccgccc cgaccaagcc tggtgcgatg gacgtgagtc
     3001 tttaggcgga agcatgatta acctcagttc agcgctcgaa gttgcaactg ccacccattc
     3061 cggcatggtg cggtcgcaca acgaggacag cattgcggcc gaccagggaa tcgggctggc
     3121 ggtgcttgcc gacggcatgg gcggctacaa cgccggagag gttgcaagcg gcatcgcggt
     3181 tgagctgatc aaggccgaga tggcgaaggc gctcgcgggc aagaagcccg aggaactgaa
     3241 cggtcagagc gccgagcggc tgatcgcgga gcacgcgacg cgcgctaacg ccgcgatcta
     3301 tcaggcggcc caaagccagc cgcagtattc gggcatgggc acgaccctgg tggtcgcgtt
     3361 gtggcacaac aaccaggttt cggtcgggca cgttggcgac tcccgcctgt accggttgcg
     3421 cggcgggacg ctggagcaga ttacgcgcga ccactctttg ttgcaggaac agatcgatag
     3481 cggcatgatt accaaggagc aggccaggta ttcgcagaac aagaacctgg tcacgcgcgc
     3541 ggtcggcatc gatccggagg tggaaacaga ggttcacacc tatccggcgc agccgggcga
     3601 catctacctg ctgtgctccg acgggctctc cgacatggtg accgacgagg acatccagtt
     3661 ggcgcttgcc tcgctgcagt ccaacctgcc gctcgcggca cagcagctgg tgcagcaggc
     3721 gaatgacaac ggcgggcgcg acaacgtttc ggtgatcctg gtgcgggtcg ttaagagctt
     3781 ccaggcgcgc accggctggc ttgagaaatt gaaggcttgg tttagataga aggcggggat
     3841 accaccatgg cgaagttgat tcttagtctg gaaggatcgg tgatcaggga ggtcccactg
     3901 aacaaggaac gcgtgacaat cgggcgcaag ccgcaaaacg acatccagat cgaaaatctg
     3961 gcggtgagcg gggagcacgc ctgcatcgtc acgatcctca acgattcctt cctcgaggac
     4021 ctgggcagca ccaatggaac gctcgtcaac ggcaacccga tcaagaagca cattctgcag
     4081 aacaacgatg tgatcgaaat cggcaagtac aagatgaaat acgtggtcga agccgccgcc
     4141 tccgaacacg ccccgccgga ggacttcgag aagaccatgg tgatgcgcgc cccccccaag
     4201 gccgccactc ccgccaaggc agacgccgcc ccggcggccg cacccaagcc ggcagcagcg
     4261 cctgcggcaa ccccgccgct cgcgcccaag gcggtcacgc ctgccgcagc cccagcgccc
     4321 gcgcccaagc cagcggcgcc tgcggcagcc gcggcgcccg cacccaggcc agcggcgccc
     4381 gcggcggccc cgggggcagc ccccaagccg gcggcggtcg aatcgcaacc gcttgccgcg
     4441 ctccagatcc tcactggcgc agccgccggg agggagatcg aactcaccaa gaatctcacc
     4501 accatcggca agccgggttt gcaggtggcc gtcatcacgc gacggccgca gggctactac
     4561 atcacccacg tcgaaggcgc caacttaccg gtggtcaacg gcaagaagct cgacgcccag
     4621 gcccaccagc tcaacgatca cgacgtgatc gagctggccg gcgtgaaaat ggaatttttc
     4681 gtcaagagat aggggcgccg gcaaatcggg aactcgctcc cccgccggaa caactgggcg
     4741 cggcgatatt gggccggatg tcggtacgat cccgatatcc ggcctttgtt tctgatcgtg
     4801 gtcgagttcc ggaacggttg ttgcggacgg aaatcaatgg caacggaacg gtaatcaagg
     4861 ggaagagagg atgctcgcga cgataaaaaa gcatctggcg cggatcatca tcggcctagt
     4921 agtggtgctg gtgtttctcg ttcacaccgt cgggctgtgg ggactccgat tgccgttgct
     4981 cgaaaacctt gaagcgatca tttacgacac gcgattgcgg ctcaccatgc cgggcggcgt
     5041 ggacccacgc atcgtcatcc tcgacatcga cgagaagagc cttgctgaaa aggaaaaggg
     5101 cggagaaggc cgctggcctt ggccgcgcga ccgcctggcg tcgttgctcg acgtcctgtt
     5161 cgacaagtac ggcatcgcgg tcgcgggatt cgacgtggta ttcgccgaac gcgacgagtc
     5221 ttccggcatc cgcgtgctcg agcggctggg tgcccgggag ctgcacgatg tgccccaatt
     5281 ccagtcggtg ctcaagcaga tcaagcccga gctcgaatac gacgacatct tcgcgcgcaa
     5341 gatgaaggga cgtgccgtgg tgctgggtca catattttcc agcgacgagg gaggtgcggc
     5401 accgaagaag ggggcgctgc ccgcgccgat tttttcagcc aaatcattcg ccggcagaac
     5461 gatccggacc acctcgtggc agggttacac ggcaaacctc gaagtattgc agaaaaacgc
     5521 agcgggcgca ggtcatttca actcgaacac cgatcctgac ggcatcgttc ggcgcgtgcc
     5581 catgctcgcg gagtacgacg gcggctatta cgaaccgctg tcgttgacga tagtgcgcgt
     5641 gctgctcggc tttccgccgg tcaaggcgct ttccccggag acgacagcgc ttgcccgtaa
     5701 gggctatccc gacctcgagt ggttggacgt ggggtcgctc aggattccgg tggacgacac
     5761 cgcgagcgcg ctcatcccct accgcggccg caagggcagc ttcaagtatt tctcgctcgt
     5821 cgacgttctg aacgaacgcg tcgacgtggc cgagctgaaa ggcaagatcg tgatagtcgg
     5881 cacgaccgcc cccggcctgc tcgacttgcg ggcgaccccc gtggattcgg tctaccccgg
     5941 ggtcgaggtg cacgccaaca tgattgcggg catgctcgac ggccacatca agcagcggcc
     6001 gccctatgtg cttggcgccg agttcatgct gctgttgttt acgggcctga tgatggcgct
     6061 gttgctgccg ctgctgaatc cgctccgatc gacactggtg acgctgctgg tgctggtggc
     6121 ggtgctcgcc atcaacgtca tggtgttcca gtacggccat ctggtgctgc cgctcgcctc
     6181 gggaattacg atggttctgc tgctgtttac gctcaacatg gcctacggct tcttcatcga
     6241 agcgcgcggc atgcgccaga tcaccggtct cttcggccag tacgtgccac ccgagctggt
     6301 ggagaggatg gcggataacc ccgagcgatt caacatggcg ccacgggcgg aggagctctc
     6361 ggtattattc tcggacgtgc gcgggttcac caccatctcg gaaagcctca cgcccgagga
     6421 tctggccacc tatatcaacg agtatctcac cacgatgagc ctggtaatcc gcgagcggca
     6481 ccacggcacg ctcgacaagt acatcggcga cgcgatcatg gcgttctggg gcgcgccggt
     6541 agccgatccc cggcatgcga tgaatgcggt gcaagcggca atagacatgc agagcgaggc
     6601 gaagatcctc aacgagaaat tcaaagccaa gggctggccc accttcaaga tcggcatcgg
     6661 tgtcaactca ggggtgatgc gggtcggcga catgggctcg aaaatccgca aggcgtacac
     6721 ggtgatgggc gatgccgtga acctgggctc gcggctcgaa ggcatcacaa agcagtacgg
     6781 ggctgatatt attatcggcg aaggtactaa agcggcgatc tcaggcatcg tgtgccggga
     6841 gatcgaccgg gtgcgggtga agggcaagga cgagccggtc gcgattttcc agccgttggg
     6901 gctggaaggg caggttggtc ggtcaaagct tgacgaaata aagctatgga atcaagcgct
     6961 taagtactat agagcacagc aatgggatca agccgaatta caattgctca accttaagaa
     7021 atccgctccc gacggcgcgc tgtatgatga attcgtggag cggatcggat ttctgcgagc
     7081 gcacccgccg ggggccgatt gggatggcgt gtggaaattt gagaccaagt gatgaaactg
     7141 aggattctcg gttgcagcgg cggcatcggg ggcgatttgc gcaccacttc gatgctgctc
     7201 gatcacgata cgctgatcga cgctggcacc ggggtgggcg acctctcgat caacgagctg
     7261 gcacagatcg accacgtctt cgtcacgcat tcccacatgg atcacgtcgc ttcgatccct
     7321 ttcatcgtgg ataccgtggg ctggatgcgc gacaaaccgc tcacgctgca cgcgacccgg
     7381 gaaacggtcg ccattctgaa ggaccatctc ttcaactgga aactgtggcc ggatttcacc
     7441 cagatccccg acgcgaagaa ccccgtgctg cgctacgaga cggtcacggt gggaagcgga
     7501 gccgcgttga acggccgaag gatcacgccg cttccggcca accacgtggt gccggcggtg
     7561 ggttatcatc tcgattcggg caaggcgagc ctggttttca cgggcgacac gaccagcaac
     7621 gatgcattat ggccgatcgt gaacgagatt tcgaacctga agtacctgat catcgagacc
     7681 gcgctttcgg accgcgaaca ggaacttgcg cgcatgtcga aacatctttg cccgagcctg
     7741 ctggcggaag agcttgcgaa gctcaagctc gcgccggaga tttacatcac gcatctcaag
     7801 ccgcgcgaat ccgaaatcat catgcagcag atcgcggtca aggtgaagga gcgcaggccg
     7861 cggatgctgc agaacaacca cgtgtttgaa ttttgagcgg gggacgttta ctgcaagatc
     7921 ggacgaaacg aggattacgg gacgctgtta ttcgcaagac aggaacggac agggcgaggt
     7981 cactatgagc actgtactcc agagcaagcc ggtcagtccg gaaaatctgg ccgagagact
     8041 gcaattccag caaaagctgc aggcggtcac gaacaaggtt cacgcgacca agaacatcga
     8101 cgagatcatc ctggagctct cgcaggaact gtgcaatctg ttcaacgccg accggctgac
     8161 gatctacctc acgagcgagg acaagggctc gatcatctcc aaggtgaaga ccgggctcaa
     8221 ctcgttcaag gacatcaaac tgccgatcaa cgagcagtcg atcgccggct tcgtcgccgg
     8281 caacaagcgt gtggtcaaca tccgcgacgt ctatgatgac gcggagctga aatcctacag
     8341 ccagcagctc aacttcctga aggcggtcga cgccaagacc ggctaccgca ccaagcagat
     8401 gctggtcgcg ccggtgctgg aggccaaaac caaggacctg atcggcgtgg tgcagatcat
     8461 caacgccaag accgggcatc cgttcccgcc gatcatggag gagggcgtag tacagctggg
     8521 cgagacgctc gcgatcgctt tccgccagcg ccagggcccg atgatggggg tgcgcaccaa
     8581 gtatgacggg ctggtgacca acgccgtgat ttcggcggag gagctggagc tcgcgcagcg
     8641 ctcggcgcgg cgcaagaacc tcgatctcga gacgattctg atcgacgagt tcaaggtcaa
     8701 cgtgccggcg atgggcagcg ctctgtcggg gtattttggc gtgccctatg agccgtacag
     8761 gcaggaccgc atcaagccgc cggatctcct gaagaacatg agccgcgagt tctgccagac
     8821 caaccagtgg gtcccgctcg aggacgccaa ggaaggcctc atcgtgatgg ctatcgaccc
     8881 cgagaagacc aaggcgtcga agcaggtcaa caacgtctat cccaagggca agatcgtgtt
     8941 ccgggtcacg accaaccgcg aattcgcgag cacgctcgac cagatgttcg gcggcggcgg
     9001 cgtcggcgca ttggcggaca gtggggacat cggcgacatc ctcggcacgc tcgatgatga
     9061 aagcgcggtg gtcgaggggg cgccggaaga cgtcgcttcg gcggcgaacg acaatgagct
     9121 ggtgaagctc gtcaacaaga tcatcatcga tgcctatcag cagggggcct cggacatcca
     9181 catcgagccc taccccggca aggccaagac
//
LOCUS       DBNGWZ010000082         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2778194, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000082 DBNGWZ010000000
VERSION     DBNGWZ010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2778194"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16CX_02215"
     CDS             complement(<1..197)
                     /locus_tag="AC16CX_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HIR3011356.1"
                     /translation="MNAVTERIPEPLVFTDAAAAKVKSLIDEEGNPNLKLRVFVQGGG
                     CSGFQYGFTFDEEAGDEDTRM"
     gene            complement(237..644)
                     /locus_tag="AC16CX_02220"
     CDS             complement(237..644)
                     /locus_tag="AC16CX_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR3011357.1"
                     /translation="MFGSKSNKPHNNIECLIGAGTTIEGNITFTGGLRVDGRVRGDVI
                     AADGKPGTVVLSEQAQIEGEIRVSHVVINGTVVGPVHATEYVELQPKANVTGDVYYKT
                     LEIQLGAVVQGRLVYQNDGKSEKVVQLKPATAE"
     gene            complement(656..1405)
                     /locus_tag="AC16CX_02225"
     CDS             complement(656..1405)
                     /locus_tag="AC16CX_02225"
                     /inference="COORDINATES: protein motif:HMM:NF042896.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HIR3011358.1"
                     /translation="MAQLIRALQRKFGISAPHMAVRAHVPWYWRWLGYVVLGALVVGV
                     GWTTYDYGMEFAGFRQSEAARALARLNDEIRSRETQITELRSQVAAAERQMQIEHATY
                     GDLARQVKTLTEENAALREDLAFFQSLMAAGGKEGALSINRFRIQPEALPGEYRYRLL
                     LVQTGQRVKEFHGTLQFVLNLEQGDRKLVLTLPPEGEKNVKEYQISFKFFQRVEGTFR
                     VAPGAVVKSLQVRVFENGSVAPKLSHTVSVS"
     gene            complement(1406..2446)
                     /gene="argC"
                     /locus_tag="AC16CX_02230"
     CDS             complement(1406..2446)
                     /gene="argC"
                     /locus_tag="AC16CX_02230"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3011359.1"
                     /translation="MAKNGIRIGIVGGTGYTGVELLRLLAQHPHCELAAITSRKEAGI
                     AVADLFPNLRGRVSLKFSDPAAAALDRCDLVFFATPNGVAMEQARALVDGGVRIIDIA
                     ADFRIRDVALWEQWYGMKHACPELVAEAVYGLPEVNRERIREARLVANPGCYPTAVQL
                     GLLPLVEAGVVDLDHLIADAKSGVSGAGRKAEVHTLLAEASDNFKAYGVPGHRHHPEI
                     AQGLTRAAGRPVNVVFVPHLTPMIRGIHATLYARLTATADLQALYEKRYAKEAFVDVM
                     PAGSHPDTRSVRGANMCRIAVHQPRGGDIVVVLSVIDNLVKGASGQAVQNMNIMFGLP
                     ETAGLEQLALLP"
     gene            complement(2513..2905)
                     /gene="rpsI"
                     /locus_tag="AC16CX_02235"
     CDS             complement(2513..2905)
                     /gene="rpsI"
                     /locus_tag="AC16CX_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3011360.1"
                     /translation="MQRQHHYGTGRRKTAVARVYIKPGKGDITVNGRPVDEFFTRETG
                     RMVVRQPLVLTNTLSSFDILVNVDGGGESGQAGAVRHGITRALIQHDASLKPALKKAG
                     LVTRDAREVERKKVGLHKARRRGQYSKR"
     gene            complement(2919..>3149)
                     /locus_tag="AC16CX_02240"
     CDS             complement(2919..>3149)
                     /locus_tag="AC16CX_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL13 family ribosomal protein"
                     /protein_id="HIR3011361.1"
                     /translation="RKAQNKIYYRHSGYPGGVYATPFAKLHARHPDRVLKLAVKGMLP
                     KGPLGYAMLKKMKVYAGASHPHTAQQPKPLEV"
BASE COUNT          480 a         1066 c         1008 g          595 t
ORIGIN      
        1 tccatccgcg tgtcttcatc gccggcctcc tcgtcgaagg tgaaaccgta ctggaaaccg
       61 gagcagccgc cgccctgcac gaaaacgcgg agcttgaggt tgggattgcc ctcctcgtcg
      121 atcaggctct tcaccttcgc cgcggcggca tcggtgaata ccagtggttc ggggattctt
      181 tccgttactg cattcatatc aatgctcctt gaaccaataa accggctcgg cgcgcctcat
      241 tcggccgtag ccggtttcaa ctggactact ttctccgatt tgccgtcgtt ctggtaaacg
      301 agcctgccct gcaccaccgc gccgagctgg atctcgaggg tcttgtagta tacatcccct
      361 gtgacattgg ccttgggctg cagttccacg tactccgtgg cgtgcaccgg cccgaccacc
      421 gtgccgttga tcaccacgtg ggaaacccgg atctcaccct cgatctgcgc ctgctcggac
      481 agcaccacgg tgccgggctt gccgtcggcg gcgatcacgt cgccgcgcac gcgcccgtcc
      541 acgcgcaggc ccccggtgaa ggtgatgttg ccttcgatgg tggtgccggc gccgatcagg
      601 cactcgatat tgttatgggg cttgttgctc ttgctgccga acatgatcct gctcctcagg
      661 aaacgctcac ggtatgcgat agtttaggcg caaccgagcc attttcaaag acccgcactt
      721 gcaggctctt taccaccgcc ccgggggcca ccctgaaggt gccctcgacg cgctggaaaa
      781 acttgaagct gatctggtat tccttcacgt tcttttcgcc ttccggcggc agggtgagca
      841 cgagcttgcg gtcgccctgc tccaggttga gcacgaactg cagcgttcca tggaactcct
      901 tcacgcgctg gccggtctgc accagcagca gccggtaacg gtactccccc ggcagcgcct
      961 ccggctggat gcggaagcgg ttgatgctga gcgcgccctc cttgccgccg gccgccatca
     1021 acgactggaa gaacgcgagg tcctccctca gcgcggcgtt ctcctcggtc agggtcttga
     1081 cctgccgggc gaggtcgccg taggtcgcgt gttcgatctg catctgccgc tcggccgccg
     1141 ccacttgcga gcgcagctcg gtgatctggg tttcgcggct ccggatctcg tcgttgagcc
     1201 tcgccagcgc gcgcgccgcc tcgctctggc ggaagccggc gaactccatg ccgtagtcgt
     1261 aggtcgtcca gcccaccccg acgacgagcg cgccgaggac gacgtagccg agccagcgcc
     1321 agtaccacgg cacgtgcgcc cgcaccgcca tgtggggcgc ggaaatcccg aatttgcgct
     1381 gcagcgcgcg gatgagctgt gccattcagg gaagtaaggc gagttgctcc agcccggcgg
     1441 tttccggcag tccgaacatg atattcatgt tctgcaccgc ctggcccgac gcgcctttca
     1501 ccagattgtc tatcaccgaa agcacgacca cgatatcgcc gccgcgcggt tgatgcacgg
     1561 caatacggca catgttcgcg ccgcgcaccg agcgcgtgtc cgggtggctg cccgccggca
     1621 tcacgtccac gaacgcttcc ttcgcatagc gcttctcata gagcgcctgc aaatcggccg
     1681 tggccgtcaa gcgcgcgtaa agcgtggcgt ggatgccgcg gatcatcggc gtgagatgcg
     1741 gcacgaagac gacattgacg gggcggccgg cggcccgcgt caatccttgc gcgatctcgg
     1801 ggtggtggcg gtgtcccggc acgccgtagg ccttgaagtt gtcggaggcc tccgcgagca
     1861 gcgtgtgcac ttccgccttg cgcccggcgc cggagacgcc cgatttcgcg tcggcgatca
     1921 ggtgatcgag gtcgacgacc cctgcctcga ccagcggcag gagaccgagc tgcaccgcgg
     1981 tcggatagca gccggggttg gcgacgaggc gcgcttcacg gatgcgttcg cggttcacct
     2041 ccggcaagcc gtacaccgct tccgccacga gctccgggca ggcgtgcttc atgccgtacc
     2101 actgctccca caacgcgacg tcgcggatgc ggaagtccgc cgcgatgtcg atgatccgca
     2161 cgccgccgtc caccagcgcc cgcgcctgct ccatcgcgac gccgttgggc gtggcgaaga
     2221 acacgaggtc gcagcgatcc agcgccgcgg ccgccggatc gctgaacttc agcgagaccc
     2281 ggccgcgcag gttcggaaac aaatccgcga cggcgatgcc cgcttccttg cgcgaggtga
     2341 tcgcggcgag ctcgcagtgc ggatgctggg cgagcagccg cagcagctcg acgccggtat
     2401 agccggtgcc gccgacgatg ccgatccgga tgccgttctt ggccatggtc ctgtttgcgg
     2461 gtccacagac aacaaagccg cccgcaggcg gcctggttgc gggttgaata gtttaccgct
     2521 tcgagtactg accgcggcgg cgcgccttgt gcaggcccac cttcttgcgc tcgacctcgc
     2581 gcgcgtcgcg cgtcaccagc cccgccttct tgagcgcggg cttgagcgag gcgtcgtgct
     2641 ggatgagggc gcgcgtgata ccgtgccgga ccgcgccagc ctgccccgac tcgccgccgc
     2701 cgtccacgtt gaccaggatg tcgaagctcg agagggtgtt ggtcaatacc agcggctggc
     2761 gcacgaccat gcggccggtc tcgcgcgtga agaattcgtc gaccggcctg ccgttgacgg
     2821 tgatgtcgcc cttgccgggc ttgatgtaga cgcgcgccac cgccgtcttg cggcgaccgg
     2881 tgccgtagtg atgctgccgt tgcatggcca tggtgcctct agacctccag tggtttcggc
     2941 tgctgcgccg tgtgcgggtg gctcgccccc gcgtacactt tcatcttctt cagcatggcg
     3001 tagccgagcg ggcccttggg cagcatgccc ttgaccgcga gtttcagcac gcggtccgga
     3061 tggcgcgcgt gcagcttggc gaatggggtg gcgtagacgc cgcccggata gccgctgtga
     3121 cggtagtaga tcttgttctg cgcctttct
//
LOCUS       DBNGWZ010000083        19442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2148303, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000083 DBNGWZ010000000
VERSION     DBNGWZ010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2148303"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="AC16CX_02245"
     CDS             complement(<1..141)
                     /locus_tag="AC16CX_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIR3011362.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALKEEGYRV
                     ILV"
     gene            complement(192..1370)
                     /gene="carA"
                     /locus_tag="AC16CX_02250"
     CDS             complement(192..1370)
                     /gene="carA"
                     /locus_tag="AC16CX_02250"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3011363.1"
                     /translation="MSRVPAILVLKDGTVFRGTSIGAEGLAVGEVVFNTAITGYQEIL
                     TDPSYCRQIVTLTYPHIGNTGTNPVDVESDRIYAAGLVIRDLPLLSSSWRKQWTLSDY
                     LKQHGVPAIADIDTRRLTRLLRDKGAQDGCLMAGKVNEKEALRAAHGFPGLVGMDLAK
                     VVSCDKPYEWEEGVWALGQGYGRLAEPRFHVVAYDYGIKRNILRKLASRHCRVTVLPA
                     RTAAEEALAMKPDGIFLSNGPGDPEPCDYAIRAIRRFLDIGIPTFGICLGHQLMGLAS
                     GAKTLKMKFGHHGANHPVQDLDTGRVMITSQNHGFAVDPATLPPTARVTHVSLFDGSL
                     QGFARTDRPAFCFQGHPEASPGPHDVDYLFDKFVRLMEEHPKSEVESRKSEGVGHAAA
                     "
     gene            complement(1582..2394)
                     /gene="dapB"
                     /locus_tag="AC16CX_02255"
     CDS             complement(1582..2394)
                     /gene="dapB"
                     /locus_tag="AC16CX_02255"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3011364.1"
                     /translation="MAELRVAIAGAAGRMGRALVETVLRAPDLKLAAALEQKGNPVVG
                     KDAGELAGSPCGVRIGDDVANAISGCDVFIDFTRPEGALAHLAACCKQGVRMVIGTTG
                     FSEAQKKEIAGGARDIAIVMSPNFSVGVNVAFRLLDVAAKALGGGYDVEIVEAHHRHK
                     VDAPSGTALRMGEVVAQALGRDLKQHAVYGREGVTGERKDETIGFATVRGGDIVGDHS
                     VMFIGTGERLEISHHASSRANFANGALRAARFVVHKKNGLFDMADVLGFAKG"
     gene            complement(2412..2864)
                     /locus_tag="AC16CX_02260"
     CDS             complement(2412..2864)
                     /locus_tag="AC16CX_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR3011365.1"
                     /translation="MKLRNVMAVVLLLAGCRDMPMLPAITPYRMDVQQGNFVTQEMIA
                     KVQPGMTRSQVRFALGTPLVVDPFHGDRWDYIYLYQKAGVVTEQRRIVVIFKDDKLVR
                     IEGDVVPAAPGGAAPGSAKEDKPAAAAAGVSQAKEEKPVPASGDRGIQ"
     gene            3001..3435
                     /gene="fur"
                     /locus_tag="AC16CX_02265"
     CDS             3001..3435
                     /gene="fur"
                     /locus_tag="AC16CX_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HIR3011366.1"
                     /translation="MTSPNDLKTIGLKATLPRLRILELFEKSPVRHLSAEDVYKTLSK
                     EGTDTGLATVYRVLTQFEQAGLLIRHHFETGKAVFELNQGGHHDHLVCVQCGHVEEFF
                     DSEIEKRQEKIAKDRGFKIHDHSLHIYADCTRANCPYRNQEA"
     gene            3577..3861
                     /locus_tag="AC16CX_02270"
     CDS             3577..3861
                     /locus_tag="AC16CX_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011367.1"
                     /translation="MTVAAVAIALLILWKLLAWLIKPLLVWLEEMKLRARDRKVADTE
                     TMAQYKLDDSKLHSGTAPENVEQQIRDALAEKKKTDQQQRHHQLMGDKKR"
     gene            4205..5158
                     /locus_tag="AC16CX_02275"
     CDS             4205..5158
                     /locus_tag="AC16CX_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011368.1"
                     /translation="MIRIALIGLLALICSLPATAQYPDRPLTILAGYPAGGMVDIVAR
                     LLAEGMRSKFPKGVLVANRTGAGGAVAAAEVATGKPDGYTIVLTPLSTLVIQPQILDL
                     RFKTPDDYEPVINVVSYYPLLVVQTDARWKNAQEFVAEAKANPGKMRVGSPGEGTSSH
                     LNLEELMHVAGIKVTHVPYRGWGESSPALLGGHIEALVAQPGEAKPMVDGKRMRALVV
                     FQNARHPAFPDAPTAKELGWNVANGVWFLLTAPKGTSAPVIKHIHDAAKAAMEEPAFA
                     AAMDLRGVDVDYRPGDRLRADLWREYKLHTVILKRIGMIKQ"
     gene            5373..6056
                     /locus_tag="AC16CX_02280"
     CDS             5373..6056
                     /locus_tag="AC16CX_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011369.1"
                     /translation="MGDYLMPRRTEITRPGTALVRTVAGRELGDMVFNTAEVALDSIL
                     DEGVLRELPIVGTLAKIARAGQSIAEELFLRKLLRFLIELKSIPAEERVKLLERYPDG
                     SDEQQDLGENLLLALERLDNVQKPTLLARFFAAYVREQIDYATFSRLAEALERFNLDL
                     LPQLRWFYTREGPAIEIPEEIEHELSLAGLVTVGLAGSGTIGGGASYRKSSLGEQFLK
                     IGYGVQPTQ"
     gene            6367..6726
                     /locus_tag="AC16CX_02285"
     CDS             6367..6726
                     /locus_tag="AC16CX_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011370.1"
                     /translation="MGMILGTCARRFPIHADRSMDIARRFEKTYAKQVQANDRATIAV
                     FDRTDPGKGEAARDRNSIFALRQAQKEMESWSEDQCRKAIIGVEGAVIAKDWEVAVGI
                     PAAMVFKQERERVPACR"
     gene            complement(7434..7949)
                     /locus_tag="AC16CX_02290"
                     /pseudo
     CDS             complement(7434..7949)
                     /locus_tag="AC16CX_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
     gene            complement(8134..8724)
                     /locus_tag="AC16CX_02295"
     CDS             complement(8134..8724)
                     /locus_tag="AC16CX_02295"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3011371.1"
                     /translation="MPYEMKTLGCDPSRLKGLSEKLIVSHYENNYGGAVKRLNAICDQ
                     LAGLEPASAPGFVINGLKREQLIAMNSMILHELYFDGLGAGGDPGAALSDAIKRDFGS
                     FERWRGEFVAMGKALGGGSGWVLLTYSHRDGQLVNQWAADHTHAVAGATPILALDMYE
                     HSYHMDYGAKAAAYVDAFMENINWSNVAKLYAQCTG"
     gene            complement(9159..9932)
                     /locus_tag="AC16CX_02300"
     CDS             complement(9159..9932)
                     /locus_tag="AC16CX_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR3011372.1"
                     /translation="MDASITASRLKSEFASAQPPLLIDVRRPPTFRAATDMAAGALWR
                     DPAQVASWAKELPRAASVVVYCVHGHEVSQGAAQALRGLGIAARYLEGGLEEGWKSAG
                     GDVIGKPKEGATRWVTRERPKIDRIACPWLVARFIDPEAEFLYVPPKEVLRTAKEKEA
                     IPYDVPDVHFSHDGELCSFDAFLKTYRLTGDPALARLAVIVRGADTARLELAPQAAGL
                     LAVSLGLSRNFKNDHEMLKHGMVVYDALYAWCKEGAPKR"
     gene            complement(10122..10841)
                     /locus_tag="AC16CX_02305"
     CDS             complement(10122..10841)
                     /locus_tag="AC16CX_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR3011373.1"
                     /translation="MNAQASGVKALTFDVFGTVVDWHGSVAREVRGLARAKGLRVNAV
                     KFAKAWRAGYRPAMDRVLRGEAPFEKIDVIHRAILEEVLKQFKVSTLTEEEKAHLNLV
                     WHRLKPWPDTVRGLKRLKSRFIIATLSNGNTSLLVNMAKHGGLPWDCVFSSDTFKLFK
                     PDPGMYLGAAGMLDLKPEEVMMVAAHKADLRAAAQQGLKTAFVKRPHEFGRNNNPDLA
                     SEPEFTVNAESFLDLADQLGC"
     gene            complement(10973..12139)
                     /locus_tag="AC16CX_02310"
     CDS             complement(10973..12139)
                     /locus_tag="AC16CX_02310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011374.1"
                     /translation="MDFSFSEEQLAIRAAIEKICARFDAAYWLKKDTEGGFPDDFHQA
                     FARDGWLGIAMPEAYGGAGLGITEAAIMLQAISESGAGMSGASAVHMNIWGVNPVVVF
                     GTDEQKRRWLPPLIAGTDRACFCVTEPDTGLDTTKLKTRAVKEGERYRVSGRKIWIST
                     AQVTNKMLLLARTTPIEAVSKPTLGLSLFYTDMDRKFVEAREIPKMGRKAVDSNQVFI
                     DGLEVPVADRIGEEGKGFEYILHGLNPERILIAAEAVGLGRAALKRAADYAKERIVFN
                     RPIGKNQAIQHPLAQRWVELEAADLLVYKAAWLYDNGKPCGAEANAAKYLAGEAGFRA
                     CETAVMTHGGMGYAREYHVERYLRESLIPRIVPVSPQLILCYIAERVLGLPKSY"
     gene            complement(12144..13025)
                     /locus_tag="AC16CX_02315"
     CDS             complement(12144..13025)
                     /locus_tag="AC16CX_02315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3011375.1"
                     /translation="MRFPDMQSFTHAGVAVAIFALVAVFTGCSTTNGRSGAGQPDPGV
                     PELVTFRSGDFTLKALLWKPPGRGPFPAVIFNHGSERSHDTHFALIGPMFAREGFVFF
                     APYRRGQGLSADQGTWVLDTLSRVAREQGMIERGKKAVELLKTEQLDDLLAALARLQG
                     MPFVDRGRIAVVGNSFGGVLTMVMAERGGGIRAAVNMAGAALNWKRSPEFKQWMFDMA
                     RNAQVPVFFIQAENDQDTGPSRELSAEMTRLGKPNRMRIFPPFGKTAEDGHSFGYLGS
                     HLWGPDVIAFLRETMGR"
     gene            complement(13076..14275)
                     /locus_tag="AC16CX_02320"
     CDS             complement(13076..14275)
                     /locus_tag="AC16CX_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011376.1"
                     /translation="MSGPLSGVRIVDLTTVVMGPFATQILADLGADVVKVEPPEGDVL
                     RHIAPMKHPGMGHIFLHHNRNKRSIVLDLKQAAGREALLRLTRNADVLIYNVRPQAMQ
                     RLHLAYEDVRAVNPSIIYVGAYGYGEGGRYAGQPAYDDLIQGMAALPSIFAEAGADRP
                     RYVPTAISDRITGLAAVNAVAAALYFRERTGKGQAVEVPMFETLAHMILGDHMSGRTF
                     DPPVEPLRYERMLAPHRAPYATKDGYVCVLVYNDKHWRSFFRLIGREEMFEADPRFSS
                     QEARSRNIGEVYAFVAEEMAKRTSAEWLRLLKEADIPASPMNTVEDLIEDPHLAETGF
                     FVMTEHPTEGRLRAMTVPGSWSQSPPAGLRPAPRLGEHSVEILREAGYADAEIEEMIA
                     RRVTLTP"
     gene            complement(14394..15368)
                     /locus_tag="AC16CX_02325"
     CDS             complement(14394..15368)
                     /locus_tag="AC16CX_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011377.1"
                     /translation="MFDRRRFFVYLIPFITAAALAPAAMAQEYPAKPLTFFVPFAAGS
                     ATDFLARALGQGITAETKQNIVIDNRPGASGFIATQAVAKAAPDGHVVLIATNTTHAA
                     NEHLFKKIPYDPVRDFAPVTALARGGQVMVVHPRVPVKTVKEFIALAKRQPGKLTFGS
                     GSSSSRVASELFRQMAGLQLVHVPYKSNPLAVNDLVGGHIDMMITDVVTGLPQVQAGK
                     LRALGVSSPKRLPNAPDLPTIAEAGVKGYELTFWFAAYLPAKTPPAVVARLRELFINA
                     TKSAPAQSFFKTTGIEPWTTTSAELAKFQASESAKWAKVIKAAGIQPE"
     gene            complement(15408..17069)
                     /gene="recN"
                     /locus_tag="AC16CX_02330"
     CDS             complement(15408..17069)
                     /gene="recN"
                     /locus_tag="AC16CX_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3011378.1"
                     /translation="MLRSLSIRDFVIVDRLELAFSPGFTVLTGETGAGKSILIDALAM
                     VLGERADAIVVRGGAERAEVSAEFDANASGELGRWLKDNDLAGDGDALLMRRVIESSG
                     RSRGFINGRAATAAQLRETGEFLVDIYGQHQHQSLLRPAAQRELLDAYGDLSRTTEKV
                     AGLYRAWQQRRENRIAFETNAAAFAAEREQLEWQAREIEALKFSAQEWPELTAEHSRL
                     AHAASLIEAAHLGVEAVSEGDGSSLAQLNAVVARLGALVEHDPGLKEIVDLLESARIQ
                     LQEAAHALRRYGERLDLDPRRLREIEQRLDAIHTAARKYRVPPEELPARLEAARARLR
                     EMGEGGNVDALRELEEEAHAACVAEARKLSAGRKKSAKKLSDQVTDAMQDLAMGGGRF
                     EVALNALTEATAFGLENVEFLVSAHKGMAPQPLAKVASGGELSRLSLAVQAVTTRVAQ
                     VPTLIFDEVDAGIGGRVAEIVGRMLKELGARHQVMCITHLPQVAATADQQWQVTKTTA
                     NGKVLSRVTVLDRNQRVEEIARMLGGVRITETTRRHAAEMLGIKS"
     gene            complement(17158..18030)
                     /locus_tag="AC16CX_02335"
     CDS             complement(17158..18030)
                     /locus_tag="AC16CX_02335"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR3011379.1"
                     /translation="MKSSFKTVALVGKYKSPEVAGPLLELAGFLERRKVKVLIDRLTA
                     SQVKKEIKYPVLSLEELGREVDLAIVIGGDGTMLNIARTFAPHDVALVGVNQGRLGFL
                     TDISVETMIATIGAILDGRFVAEERMLLEGQVFRGANKVFEALAFNDAVVSKGPKGSM
                     LEMEVRIDGQFIYNQRGDGLIVATPTGSTAYALSSGGPIVHPSLSVMALVPVSPHTLS
                     NRPVVISSSCTVEIIVRDDPEPRAHFDSHTRCDLREGDRVVVRRYPRTISLLHPEGHS
                     YYAMLREKLNWNRE"
     gene            18193..19206
                     /gene="hrcA"
                     /locus_tag="AC16CX_02340"
     CDS             18193..19206
                     /gene="hrcA"
                     /locus_tag="AC16CX_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR3011380.1"
                     /translation="MSERAQILLKTLVERYIAEGQPVGSRALSKYSGLDLSPASIRNV
                     MADLEEMGFIASPHTSAGRVPTARGYRFFVDTLLTVKPLATIEINQLEGQLHAESTQK
                     LVASASQLLSDLTHFAGVVMTPRRSAGFRHIEFLRLSDTRVLLILVTPEGDVQNRILF
                     TVKAYTPSELTEAANILNQHYAGLTFEEVRRRIRAELRQLTEDMTRLMTAALEAGSQA
                     ASESAEEVVISGESNLLDSQDLSSNMANLRRLFELFDRKTGLLQLLDSSSRAQGVQIF
                     IGGESGVEPLDECSVITASYKVEGQVVGTVGVIGPTRMAYERVIPIVDITAKLLSSML
                     TQR"
     gene            19295..>19442
                     /locus_tag="AC16CX_02345"
     CDS             19295..>19442
                     /locus_tag="AC16CX_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3011381.1"
                     /translation="MSRYAPEPPYSHGTFPRIGVLLIQLGTPEAPTAAAVRAYLKDFL
                     SDPRV"
BASE COUNT         3470 a         6235 c         6069 g         3668 t
ORIGIN      
        1 caccaggatg acgcgatagc cctcctcctt gagcgccttg cacgcctgcg ccccggaata
       61 gtcgaactcg cacgcctggc cgatcacgat cggcccggcg ccgatgatca ggatgctctt
      121 gatgtcggtg cgcttgggca tttcagaacc cgtgactcgt tggtcgttgc tcgttgctcg
      181 acccaattcg gctaggccgc ggcatgaccg actccttccg actttcgact ttcgacttcc
      241 gacttcggat gctcctccat gagccgcacg aatttgtcga agagataatc gacgtcgtgc
      301 ggaccggggc tggcttccgg gtggccctgg aagcagaacg cgggacggtc ggtgcgcgcg
      361 aagccctgca ggctgccgtc gaacagggag acgtgtgtga cacgcgcggt cggcggcagg
      421 gtcgcggggt ccaccgcgaa gccgtggttc tggctcgtga tcatcacgcg cccggtgtcg
      481 aggtcctgga ccggatggtt tgcgccgtgg tggccgaact tcatcttcag ggtttttgcg
      541 ccgctcgcga gccccatcag ctggtgcccg aggcagatgc cgaacgtcgg gatgccgatg
      601 tcgaggaacc tgcggatcgc ccggatggcg taatcgcacg gctcgggatc gccgggcccg
      661 ttggaaagga aaatgccgtc cggtttcatc gcgagcgcct cttccgcggc agtccgcgcc
      721 gggagcacgg tcacgcggca atggcgggaa gcgagcttcc tcagaatgtt gcgcttgatg
      781 ccgtagtcgt aggcgacgac gtggaagcgc ggttcggcga gccggccgta gccttgcccc
      841 agcgcccaca cgccctcttc ccactcgtag ggtttgtcgc agctcaccac cttcgcgagg
      901 tccatgccga ccagccccgg gaagccgtgc gcggccctga gcgcctcctt ctcgttcacc
      961 ttgcccgcca tcaggcagcc gtcctgcgcc cccttgtcgc gcaggaggcg cgtgagccgg
     1021 cgcgtgtcga tatccgcgat cgcgggcacg ccgtgttgct tcaggtagtc ggagagcgtc
     1081 cattgcttgc gccagctcga agacagcagc ggcagatcgc ggatcacgag tccggcagcg
     1141 tagatccggt ccgattcgac atccaccgga ttcgtcccgg tgttgccgat gtgcggataa
     1201 gtgagggtga cgatctgccg gcagtaggac ggatcggtga ggatttcctg gtagccggtg
     1261 atggcggtgt tgaacaccac ctcccccacg gcgagaccct cggcgccgat cgaagtgccg
     1321 cggaaaacgg tgccatcttt gagcaccagg atggcaggaa cccgcgacac gctaacccct
     1381 tgatttcaag tgtagataca caaagcggga cgggcttttc agcccctccc gctagaccaa
     1441 gggaattata cgcgaagcgg catggcgcgg caatcgctac gcgatgccgc gagtgacgag
     1501 tgtcgggtgt cgaggggaag agcgccttag ttagcgttct ggtctttctt cactcgacac
     1561 tcagtcctcg acactcctac ctcatccttt ggcgaagccg agcacgtcgg ccatgtcgaa
     1621 caggccgttc tttttgtgga cgacaaagcg cgccgctcgc agcgcgccgt tggcgaaatt
     1681 ggcgcggctc gacgcgtggt gggaaatctc cagccgctcg ccggttccga tgaacatgac
     1741 gctgtgatcg cccacgatgt ccccgccgcg taccgtggca aagccgatgg tttcgtcctt
     1801 gcgctcgccc gtcacgccct cgcgcccata gacggcgtgc tgcttcagat cgcggccgag
     1861 cgcctgcgcc actacctccc ccattcgcaa cgcggtgccg gaaggcgcat ccaccttgtg
     1921 ccggtggtgc gcctcgacga tctccacgtc atagcccccg cccagcgcct tggccgccac
     1981 atcgagcagc cggaacgcga cgttgacgcc gacgctgaag ttgggcgaca tcacgatcgc
     2041 gatgtcgcgc gcgccgcccg cgatctcctt cttttgcgcc tcgctgaagc cggtcgtgcc
     2101 gatgaccatc ctcacccctt gcttgcagca ggcggcaagg tgtgcaagcg cgccttcggg
     2161 ccgcgtgaag tcgatgaaga catcgcaacc cgagatcgcg tttgcgacat cgtcgccgat
     2221 cctcacgccg cagggggaac cggccagctc gccggcgtcc ttgccgacga cagggttgcc
     2281 cttctgctcg agcgccgcgg cgagcttcag atcgggtgcg cgaagcacgg tctcgaccag
     2341 cgcgcggccc attcttccgg ccgcacctgc gatggcaact ctcaattcgg ccatgagctt
     2401 tagcccgtcc tctattgaat tccccggtcg ccgctcgccg gtacaggttt ctcttccttc
     2461 gcctgcgaaa ctccggccgc cgctgcggca ggcttgtctt ccttcgcgct gccgggagcc
     2521 gcgccgccgg gagctgccgg caccacgtcg ccttcgatcc gcaccagctt gtcgtccttg
     2581 aaaatcacga cgatcctgcg ctgttcggtc actactccgg ccttctgata gagatagatg
     2641 taatcccagc ggtcgccgtg gaacggatcc acgaccagcg gcgtaccgag cgcaaaccgc
     2701 acctggcttc gggtcatgcc gggctggacc ttggcgatca tctcctgcgt gacgaagttg
     2761 ccctgctgga cgtccatccg gtacggggtg atcgcgggca gcatcggcat gtcccggcaa
     2821 ccagcgagga gcaggacgac ggccatgaca ttgcgcagtt tcatctacgg gatacgcttc
     2881 gtgatcgtga aatccttgct tcgcggaacg gatggaataa ccccgaccgg cgcgcggttt
     2941 gcaaggtggt gaaatacgaa tatgataacc gaccccccat cattccgaca gggacgagtg
     3001 gtgacttccc ccaacgatct caagacgata ggtctgaagg cgacgctgcc gcgcctgcgc
     3061 atactggagc tgttcgagaa gagcccggtg cgccatctct cggccgagga cgtctacaag
     3121 acgctctcca aggaaggcac cgacaccggg ctcgcgacgg tgtaccgcgt gctcacccag
     3181 ttcgagcagg cgggactgct gatccggcac cacttcgaaa ccggcaaggc ggtgttcgaa
     3241 ctcaaccagg gcggtcatca cgaccatctg gtgtgcgtcc agtgcgggca cgtcgaggag
     3301 ttcttcgact ccgagatcga gaagcgccag gagaagatcg cgaaggaccg cggtttcaag
     3361 atccacgacc actcgctgca catctacgcc gattgcacgc gggcgaattg cccctacagg
     3421 aaccaagaag cgtaaccagt ggccagtgac cggtgaccgg cccacgtgga aacgctgagg
     3481 caaataacca gacagcttga ggcgtggtgg gcttacgcgt tgcggtgcct ggacgccccc
     3541 ttttccgcgc cggcgtgcaa gcccttctgg acgtgggtga cggtagccgc cgtcgcgatc
     3601 gccttgctca tcctctggaa gctcctcgcc tggctgatca agccgctcct ggtctggctc
     3661 gaggaaatga agttgcgggc gcgggaccgg aaagtcgctg acacagaaac catggcgcag
     3721 tacaagctgg acgacagcaa gctccattcg ggcacggccc ccgagaacgt cgaacaacag
     3781 atccgggatg cgctggccga gaagaagaaa accgaccaac agcagcgcca tcatcaattg
     3841 atgggcgaca agaagcgtta aatccggatg catggcgccg gtttgagcgc gctggaaggt
     3901 tgagcaggtg cccgcttgcg ggtgcgaccc aaggagggcg cgtcggagcg ttgagcaggg
     3961 gccccgctcg cggggatgcg accgcgtaga cgcgcaaagc agccgacacc gtagatcggg
     4021 attcttggct caagcggtgc ggaggctgca cacatgcggc cgatgcgatc tcagtagatg
     4081 cttgccaatg caccattcag aggccgaccg tgctcgaacg gacgatataa tcaccgttca
     4141 acacgcagac gaaatctgga atccgttcca gtaatcgcgc cgccaacctt ctaaaggagg
     4201 gcccatgatc cgcatcgcac tgatcggtct tcttgcgctg atttgctccc tgcccgcaac
     4261 agcgcaatat cctgaccggc ctctcacaat cctcgcgggc taccccgccg gcgggatggt
     4321 ggacatcgtc gcgcgcctgc tcgccgaggg catgaggtcg aagtttccga agggggtgct
     4381 ggttgccaac cgcacgggcg ccggcggcgc agtggctgcc gcggaggtcg ccacgggcaa
     4441 gccggacggc tacaccatcg tgctcacgcc cctatccacg ctcgtgatcc agcctcagat
     4501 cctcgatctg cgcttcaaga cgccggacga ctacgagccc gtcatcaacg tggtgtcgta
     4561 ctacccgctg ctggtggtgc aaaccgacgc gcgctggaag aacgcccagg aattcgtggc
     4621 ggaagcgaaa gcgaacccgg gcaagatgcg cgtcggctca ccgggcgagg gcacttcgag
     4681 ccacctgaat ctcgaggaat tgatgcacgt agccgggatc aaggtcacgc acgtcccgta
     4741 ccgcggctgg ggcgagtcgt ccccggcgct gctcggcggc cacatcgagg cgctcgtcgc
     4801 acagcccggc gaagcgaaac cgatggtgga cggcaagagg atgcgcgcgc tggtggtatt
     4861 ccagaacgcg cgccatcccg ctttcccgga tgcgccgacc gccaaggagc tggggtggaa
     4921 cgtcgcgaat ggcgtgtggt tcctgctgac ggcgccgaag ggcacgtccg caccggtgat
     4981 caagcacatc cacgatgcgg ccaaggcggc gatggaagag cccgcgttcg cagccgcaat
     5041 ggatttgcgc ggcgtggacg tggactaccg ccccggcgac aggctgcgcg cggacttgtg
     5101 gcgggaatac aagctccaca cggtgatcct gaagcggatt ggaatgatta agcagtgacg
     5161 cagtcgcaag tgtcgagtga cgagtgtcga gtgaggacta gaaccggaaa atagccgcca
     5221 agtgctgtta aagccggacg cggagcgcgg gtttaactcc gcgttttcga acggacgaag
     5281 tagcagccgc cgttcaacac gcggtgccgc ctcatcgctg catgtcaact aaattgtcaa
     5341 ctaataaatc aactaatttg tcaaccaatt cgttgggcga ctatctgatg ccgcgtcgca
     5401 cagaaattac tcggccggga accgcgctag tgagaaccgt ggcaggtcgg gagctgggcg
     5461 acatggtgtt taacaccgct gaagttgcat tggatagcat cttggacgaa ggcgtactta
     5521 gagagttgcc cattgtcgga acgctcgcga agattgcccg ggccgggcaa tcgatcgcag
     5581 aagaattgtt tctgcgtaag ttgttgcgct ttctcatcga gctaaaaagc attcccgcag
     5641 aagaacgagt gaagttacta gagcgttatc cagatggctc cgatgaacag caggatctag
     5701 gtgagaactt gctattggcc ctagagcgtc tggacaatgt acagaaacca acactgctcg
     5761 cgcgtttttt tgcagcgtac gttcgagagc agattgatta cgcaacgttc tcgcggctcg
     5821 cggaagcgtt agagcgcttt aatctcgacc tgctaccaca acttcgctgg ttttacactc
     5881 gtgaaggccc ggctattgaa attccagaag agattgagca cgaacttagt ttggccggtt
     5941 tggttacggt gggccttgcc ggctcaggta ccattggcgg aggtgcgtcc taccgaaagt
     6001 cttccctggg ggagcaattt ctcaaaatcg gctacggtgt gcagccaacg caatgactcg
     6061 cccagccatc cgttcaacac gaacgcgctg tgaccggcgc accggttaac ggcacattgg
     6121 acggcaatta agtgatcgcg gcgtgttgag atctgcactg ttaacgggcg acccattctt
     6181 ataaagcttg gaggcagcat agaggatgac cagcatatga gacgaaaggc accatctaga
     6241 ttcgtggcat tggcagtgct gggattggca gtatgcctct cgggtcttaa cgcatacgcg
     6301 tatcgcggct tttgcgtgtc ggacgcagag ctgagggaag ttcttattgg catatactca
     6361 cgcaacatgg gaatgattct tgggacctgc gcacgtcggt ttcccattca tgctgacaga
     6421 tccatggata ttgcaaggag gtttgagaaa acctacgcga agcaagttca agcaaatgat
     6481 cgtgcaacta tcgcggtatt tgatcggacg gatcctggga aaggggaagc cgcgagggac
     6541 cgaaatagca tcttcgcgct tcgtcaagcg caaaaggaga tggaatcttg gtcggaagat
     6601 caatgccgta aggccatcat tggtgttgaa ggagccgtca ttgcaaaaga ttgggaggtc
     6661 gctgtcggca ttccagcagc tatggtattt aagcaggaac gcgaacgcgt tcctgcctgt
     6721 cggtagcgga atcggccgtg atcgaggccc gcgtgtacca gctacgcgat tgtcttttga
     6781 gcgtacctct gcgtccagat gaatctgaga aaagtagtat ggcgaaccct taaggcctat
     6841 tggcgtgact ggccgcccaa gcttagctgg aacggacggc ccgcaagcgc ggtgccatca
     6901 ttggcgatcg taccggcccc cgttcaaaac cacgttagag ttcgcgaagg gagcgcgata
     6961 tgtcttggca tcgaattgtc ttaacgaacg atcaaatact cgcgggcgaa cacgtgaata
     7021 tacaagatgc gtttaccgct ctttttacgg cggcactggc acctaaagaa gcggcattgt
     7081 tcagcgctgg ccacccggcg gacaactacg cgctctattt ttctcctggc tcgtacccct
     7141 tcgccaagac cctgatttct tcaaaaggcg gcgtatcgtg cgacgccccg cccaaggaag
     7201 gtacgtcact tctcgtcggc cacgccggtg cccgagatgc tttactatgt cgcaggttcg
     7261 caggttcggg gtcaggtctt tactttatag tttttcgtag ctgtaagtta acgacatacc
     7321 ccgtaccctt caccggtcac cggtataagt cggaattcca ggtgtgcgtc tcgtcccgcg
     7381 cctccttgca ccaagcgcaa agcgcgtcgt atatcacgaa atgtgttccc gcgttacctt
     7441 tgggcgcact gatcgaagag cctgatgacg ctcgcccagt tgatattctc catgaaggcg
     7501 tccacgtagg cggcagcctt ggcgccgtag tccatgtggt atgaatgttc gtacatgtcg
     7561 agggcaagga tcagcgtcgc gccggccaca gcatgcgtat ggtcggccgc ccactgattg
     7621 atcagctgtc cgtcgcggtg ggagtaagcc agcaataccc accccgaccc gccaccgagc
     7681 gcctttccca tggccacaaa ctcgctgcgc cagcgctcga agcttccgaa atccctcttg
     7741 atcgcatccg acagggcggc gcccggatcg ccgccagcgc ccagcccatc gaagtaaagc
     7801 tcgtggagga tcatcgagtt catcgcgatc agctgctcgc gcttgaggcc attgatcaca
     7861 aacaccggcg cattcgccgg ctccaagccg gcaagctgat cgcagatcgt gttgagccgc
     7921 ttgaccgcgc cgccgtagtt gttttcaaaa cggggtcaag tcttgccttt tgcctaaact
     7981 ggaatggcca tcgttgccgt tatggcataa gtcaagacct gacgttggcg ctttacgatc
     8041 gttaatcggt tctcttggcg ggcttggcgg ttcaattatt gaatctctct cctcgtctct
     8101 gcggttcaat attgatttgc tcttctcggc ggtttagccc gtgcactgcg catagagttt
     8161 ggccacgttt gaccagttga tattctccat gaaggcgtcc acgtaggcgg cggccttggc
     8221 gccgtagtcc atgtggtacg agtgctcgta catgtcgagg gcgaggatcg gcgttgcgcc
     8281 cgccacggca tgcgtgtgat cggccgccca ctggttgacg agctgcccgt cgcggtggga
     8341 gtaggtgagc agcacccagc ctgagccgcc gccgagcgct tttcccatcg ccacaaattc
     8401 gccgcgccag cgctcgaagc tgccgaaatc ccgcttgatc gcatccgaca gggcggcacc
     8461 cggatcgccg ccggcgccca gcccatcgaa gtaaagctcg tggaggatca tcgagttcat
     8521 cgcgatcagc tgctcgcgct tgaggccatt gatcacgaac cccggcgcac tcgccggctc
     8581 caggccggca agctgatcgc agatcgcgtt cagccgcttg accgcgccgc cgtagttgtt
     8641 ttcgtaatgg ctgacgatca gcttctccga cagccccttc aggcgcgacg gatcacagcc
     8701 tagcgttttc atttcgtacg gcatttgcct ctccttagcc aaaatattgg ctcgatcttg
     8761 tttctcttcg cgggcttggc ggttcgttat tgttcttgtt tctctctgcg cctctgcggt
     8821 tcaattattg atcttgcttc tctcagcggt tagcggtaag ccgtgggatt ccaggtgtgc
     8881 gtttcctccg cgcccgcgga gggcggagca ggttccaagc gcatcatgct tggatgcgac
     8941 cccggagcgg gatgcagcgc cgcaatggcc cgcgtgctta cgtgcgcctt cacggtcgca
     9001 tccccataaa atggggcgcg tcgaggggcg gaagagggac cctctgggat ctgacccctg
     9061 agacgcgcat gggatcggca ttgaatgacg cgcccttgat cggcttcacg gtcgcatccc
     9121 gcaagcgggc ccctgctcca cgttgcgccg atccccgctc aacgtttcgg cgcaccctcc
     9181 ttacaccacg cgtagagcgc gtcgtacacg accatcccat gcttgagcat ctcgtggtcg
     9241 tttttgaagt tgcgcgacag gccgagtgag accgcgagca gtcctgccgc ctgcggcgca
     9301 agttccagcc gcgccgtgtc ggcgccgcgg acgatcacag cgagtcgggc cagcgcagga
     9361 tcgcccgtga gccggtaagt tttcaggaaa gcgtcaaagc tgcacagctc gccgtcgtgc
     9421 gagaagtgca cgtccgggac gtcgtaggga atcgcttcct tctcctttgc cgtgcgcaat
     9481 acttccttgg gcggcacgta gagaaactcc gcctccggat ctatgaagcg tgcgacaagc
     9541 cacgggcacg cgatgcggtc gattttcggt cgttcgcgcg tgacccagcg cgttgcgcct
     9601 tctttcggct tgccgatgac gtcaccgccg gcggacttcc agccttcctc gaggccaccc
     9661 tcaagatagc gggcagcgat cccgagcccg cgcagcgctt gcgccgcgcc ctgactcact
     9721 tcgtgcccgt gcacgcagta aacgacgacg cttgccgcgc gcggcaactc cttcgcccat
     9781 gaagcaacct gggccgggtc gcgccacagc gcaccggcgg ccatgtcggt cgccgcgcgg
     9841 aacgtgggag gccggcgaac gtctatgagc agtggcggtt gcgcgctcgc gaattcggac
     9901 ttcagtcggg atgcggtaat ggacgcgtcc atggcttctg actcctctca gaatcgttca
     9961 gaagcagagc ttggacgcgg gaagcgtctt gggtagcggg gagtctgcga aatccccgcg
    10021 taaaaacagt ctatccccgc ggcgtacgat ggtcaacgtc tatcggtttg cggtcaattg
    10081 ctgtccttga ttctctcggc gtgctcggcg gttcaaattg ttcagcagcc gagctgatcg
    10141 gcaagatcga ggaagctctc ggcgttgacg gtgaactccg gctcggaggc gaggtccggg
    10201 ttgttgttgc gcccgaattc gtgcgggcgc ttgacgaacg cggtcttgag cccctgctgc
    10261 gccgccgcgc gcaggtcggc tttgtgggcg gcgaccatca tcacctcttc cggcttcagg
    10321 tccagcatgc ccgccgcgcc gaggtacatc ccggggtcgg gcttgaagag cttgaaggtg
    10381 tcggaggaaa agacacagtc ccacggcagg ccgccgtgct tcgccatgtt cacgagcagg
    10441 ctggtgttgc cgttggacag agtagcgatg atgaatctcg acttgaggcg cttcagtccg
    10501 cgcaccgtgt cgggccacgg cttgagacga tgccacacca ggttgaggtg cgccttttct
    10561 tcctctgtca acgtcgagac cttgaactgc ttcagcacct cctcaaggat cgcgcggtgg
    10621 atgacgtcga tcttctcgaa cggcgcctcg ccgcgcagca cccggtccat cgccggccgg
    10681 tagcccgcgc gccaagcctt ggcgaatttc accgcgttga cccgcagtcc cttcgcgcgg
    10741 gccagcccgc gcacctcgcg cgcgaccgag ccgtgccagt ccacgaccgt gccaaagaca
    10801 tcgaaagtga gggccttgac gccagacgcc tgcgcattca tcggattgac tcccaaagtg
    10861 cgtgagtggt gagtgggcct aatcttagaa accggcgtac ttccgcgcgt tgaacggcga
    10921 gcggtgtcgt ccgttcgaac gcgtggaatt aaaccgaagt ggcgcttccg gtttaataag
    10981 acttcggcag ccccagcacc cgctccgcga tatagcacag gatgagctgc gggctgaccg
    11041 gcacgatgcg cgggatcaac gactcgcgca ggtagcgctc gacgtggtac tccctcgcgt
    11101 agcccatgcc gccgtgcgtc attaccgccg tctcgcacgc ccggaaaccc gcttcgcccg
    11161 cgagatactt cgccgcgttc gcttccgcgc cgcacggctt gccgttgtcg tacagccagg
    11221 ccgctttgta tacgagcaga tccgccgcct cgagctcgac ccagcgctgc gcgagcgggt
    11281 gctggatcgc ctggttcttg ccgatcggtc ggttgaacac gatgcgctcc ttcgcgtagt
    11341 cggccgcgcg cttcagcgcc gcccgcccga gcccgaccgc ttcggcggcg atcaggatgc
    11401 gctcgggatt caagccgtgc aggatgtact cgaagccctt gccctcctcc ccgatgcggt
    11461 cggcaaccgg cacctcgagc ccgtcgatga acacctggtt ggagtccacc gccttgcggc
    11521 ccatcttcgg gatctcgcgc gcctcgacga acttgcggtc catatcggta taaaagaggg
    11581 agagccccag agtcggcttg ctcactgcct cgatcggcgt cgtgcgcgca agcagcagca
    11641 tcttgttggt gacctgcgcg gtggagatcc agatcttgcg gcccgatacc cggtagcgtt
    11701 cgccctcctt caccgcccgg gtcttgagct tggtggtatc gaggccggtg tcgggctcgg
    11761 tgacgcaaaa acacgcgcgg tcggtgccgg cgatgagcgg cggcagccag cgccgcttct
    11821 gctcgtcggt gccgaaaacg accaccgggt tgacgcccca gatgttcatg tgcacggcgg
    11881 atgcgccgga cattccggcg ccggactcgg agatcgcctg caacatgatc gccgcctcgg
    11941 tgatgccgag acccgccccg ccgtacgcct ccggcatcgc gatgccgagc cagccgtcgc
    12001 gcgcgaaggc ctggtgaaaa tcgtccggga agccgccctc tgtgtccttc ttcaaccagt
    12061 aggcggcatc gaaccgcgcg cagatcttct cgatcgccgc gcggatcgcg agctgctcct
    12121 cggaaaacga gaagtccatg ctcctagcgc cccatcgtct cccgcaggaa cgcgatcacg
    12181 tccgggcccc agaggtgact gccgagatag ccgaagctgt ggccgtcctc cgcggtcttg
    12241 ccgaacggcg ggaagatccg catgcggttg ggcttcccga gccgggtcat ctccgccgac
    12301 agctcccggc tcgggccggt gtcctggtca ttctcggcct ggataaagaa caccggtacc
    12361 tgcgcgttgc gcgccatgtc gaacatccac tgcttgaatt ccggggagcg cttccagttg
    12421 agcgccgccc ccgccatgtt gaccgccgca cggatgccgc cgcctcgttc cgccatcacc
    12481 atggtgagga cgcctccgaa ggaattgccc acgacggcaa tgcggccccg gtcgacgaac
    12541 ggcatgccct ggagccgggc gagcgccgcg aggaggtcgt ccagttgctc ggtcttgagc
    12601 agctcgaccg ccttcttgcc gcgctcgatc ataccctgtt cccgcgcaac gcgggacagc
    12661 gtgtcgagca cccacgttcc ctggtccgcg gagagtccct gtccccggcg gtacggcgca
    12721 aagaatacga aaccctcgcg cgcgaacatc ggaccgatca acgcgaagtg agtatcgtgc
    12781 gagcgctcgc tgccatgatt gaatatcacc gcgggaaaag gtccgcgccc cggcggcttc
    12841 cacagcaggg ccttgagcgt gaagtccccg ctcctgaacg tgacgagctc gggcacacct
    12901 ggatcgggct gcccggcccc actgcgcccg ttggtcgtgg aacagcccgt aaacactgcg
    12961 acaagcgcaa atatcgcgac ggcgacgccc gcgtgcgtga atgactgcat atccggaaat
    13021 ctcatcgctt gtcctccttt acccatcacc catcactcat cacccatcac tgccgtcagg
    13081 gcgtcagcgt cacgcgcctc gcgatcatct cctcgatctc ggcatcggca tagcccgcct
    13141 cgcgcaggat ctcgacgctg tgttcgccca ggcgcggcgc cggccgcaat cccgccggcg
    13201 gcgattgcga ccagctgccc ggcaccgtca tcgcgcggag cctgccttcc gtcggatgct
    13261 cggtcatcac gaaaaacccg gtctccgcga ggtgcggatc ctctatcagg tcctccacag
    13321 tattcatggg cgacgccggg atgtcggctt ccttcagcaa gcgcagccac tccgcgctgg
    13381 tgcgtttggc catttcctcc gcgacgaacg cgtaaacctc gccgatgttg cgggagcgcg
    13441 cttcctggct ggagaagcgc gggtcggcct cgaacatctc ctcgcggccg atcagccgga
    13501 agaagctgcg ccagtgcttg tcgttgtaga ccagcacgca cacgtagccg tccttcgtcg
    13561 cgtacggcgc gcggtgcggc gccagcatgc gctcgtagcg caagggttcg acgggcggat
    13621 cgaaggtgcg cccggacatg tgatcgccaa gaatcatgtg cgcgagcgtt tcgaacatcg
    13681 gaacctcgac tgcctggccc ttgccggtgc gctcccggaa atacagcgcg gctgcgaccg
    13741 cgttgacggc ggcaaggccg gtgatgcggt ccgagatcgc ggtcggcacg taacgcggcc
    13801 ggtcggcgcc cgcttcggcg aagatcgagg gcagcgccgc catgccctgg atcaggtcgt
    13861 cgtaggccgg ctgcccggcg tagcggccgc cttcgccgta gccgtaggcg ccgacgtaga
    13921 tgatgctcgg gttgactgcg cgcacgtcct cataagcgag gtgcagccgc tgcatcgcct
    13981 gcgggcgtac gttgtagatg aggacgtccg cgttgcgggt aagccgcagc agcgcctcgc
    14041 gtcccgcagc ctgcttgagg tcgagcacga tggagcgctt gttgcggttg tgatggagga
    14101 agatgtgccc catgcccggg tgcttcatgg gcgcgatgtg gcgcagcacg tcgccttccg
    14161 gcggctcgac cttgacgacg tccgcgccga gatcggcgag gatctgggtc gcgaacggcc
    14221 ccatcaccac ggtggtgagg tcaacgatac ggacgccgga caggggtccg ctcattacca
    14281 atccagcgcg cgaagaacaa acgtgagggc gggagagcgt gagcgcggga gagggaaaga
    14341 cgttcgctgg cggtttttcg atctcacgat ctcccgctct cccgacttca cggctactcg
    14401 ggctggatgc ccgccgcctt gatgaccttc gcccatttcg ccgattccga cgcctggaac
    14461 ttggcgagct ccgcggaggt ggtcgtccag ggctcgatgc cggtggtctt gaagaaggac
    14521 tgcgccgggg cgctcttggt ggcgttgatg aacagctccc gcaggcgcgc gacgacggcg
    14581 ggtggcgttt tcgccggcag gtaggcggcg aaccagaagg tcagctcgta gcccttcacg
    14641 ccggcttcgg ctatcgtcgg cagatcaggc gcgttgggca ggcgcttcgg gctgctcacg
    14701 ccgagcgcgc gcagcttccc ggcctgcacc tgcggcagcc cggtgacgac atcggtgatc
    14761 atcatgtcga tgtggccgcc tacgagatca ttgacggcga ggggattgct tttgtaggga
    14821 acgtgcacga gctgcagccc cgccatctgc cggaacagct cgctcgccac ccggctcgag
    14881 gagctgccgc tgccgaacgt gagcttgccc ggctggcgct tggcgagcgc gatgaattcc
    14941 ttgacggtct tcaccggcac gcggggatga acgaccatga cctgcccgcc gcgcgcgagc
    15001 gccgtgacgg gtgcgaagtc ccggacgggg tcgtagggaa tcttcttgaa caggtgctcg
    15061 ttcgcggcat gtgtcgtgtt ggtcgcgatg agaacaacgt ggccgtcggg cgccgccttg
    15121 gcgaccgcct gcgtggcgat gaagccgctc gcgcccggcc ggttgtcgat gacgatgttc
    15181 tgcttggttt cggcagtgat gccctgtccc aatgcgcgcg cgaggaagtc ggtcgcgctg
    15241 ccggccgcga acggcacgaa gaaggtcagc ggctttgccg gatattcctg cgccatcgcg
    15301 gcgggcgcca gcgccgcagc cgtgatgaat gggatcagat aaacgaagaa tctgcgtcga
    15361 tcaaacatga tgtctccttc agaaaaccgg aaaagcgtga atcgcgctca gcttttgatc
    15421 cccagcatct ccgccgcgtg gcggcgggtc gtctcggtta ttctgacgcc gcccagcatg
    15481 cgcgcgattt cctccacgcg ctggttcctg tccaggaccg tcactctgct caggaccttg
    15541 ccgttcgcgg tggtcttggt cacctgccac tgctggtcgg cggtcgccgc gacctgcggc
    15601 aggtgggtga tgcacatcac ctggtgcctg gctccgagct ccttcaacat cctcccgacg
    15661 atctccgcga cgcgcccgcc gatgccggcg tccacttcgt cgaaaatcag cgtcggcacc
    15721 tgggccacgc gggtggtcac ggcctggacc gcgagggaga ggcgcgacag ctcgccgccg
    15781 gaagccacct tcgcgagcgg ctgcggcgcc atgcctttat gggcggagac gaggaattcg
    15841 acattttcca gcccaaacgc ggtggcctcc gtcaacgcgt ttaacgcaac ctcgaatcgg
    15901 ccgccaccca tggccagatc ctgcatcgcg tcagtgacct gatcggacag ttttttcgcc
    15961 gacttctttc tcccggcgga cagttttctc gcctcggcca cgcacgctgc gtgcgcctct
    16021 tcctccagct cgcgcagcgc atccacgttg ccgccctcgc ccatctcccg cagccgtgct
    16081 ctcgcggcct ccagccgagc cggcagctct tcgggcggga cccgatactt gcgcgcggca
    16141 gtgtgaatgg cgtccagccg ctgctcgatt tcgcgcagcc gccggggatc gagatcgaga
    16201 cgttcgccgt agcgacgcaa cgcgtgcgcc gcttcctgca gctggatcct cgccgactcg
    16261 agcagatcca cgatctcctt cagcccgggg tcgtgctcga ccagcgcgcc gagcctggcc
    16321 actaccgcgt tcaattgcgc gaggctggag ccgtcgccct cggaaacggc ctcgacgccg
    16381 aggtgggcgg cctcgatgag gctcgcggca tgggcgagcc gtgagtgctc ggccgtcagt
    16441 tccggccatt cctgcgcgct gaacttgagc gcctcgatct cgcgcgcctg ccactcgagc
    16501 tgctcgcgct cggcggcaaa cgcagccgcg ttggtctcga aggcgatgcg attctcgcgt
    16561 cgctgctgcc acgcgcgata aagccccgcg accttttctg tagtgcgtga gagatcgccg
    16621 taggcatcga gcaactcgcg ctgtgccgca ggccgtaaca gagactggtg ctggtgctgg
    16681 ccgtagatgt ccaccaggaa ctcccccgtc tcccgcagtt gggcagccgt ggcggcgcgg
    16741 ccgttgatga agccacgcga gcggcccgaa gactcgatca cgcgccgcat gagcagcgcg
    16801 tcaccgtcgc cggcgagatc attgtccttg agccagcggc cgagctcgcc gctcgcgttg
    16861 gcgtcgaact cggcgctcac ctcggcgcgc tcggcgccgc cgcgcaccac gatcgcgtcc
    16921 gcccgctcgc cgagcaccat ggcgagcgcg tcgatcagga tggacttgcc cgccccggtc
    16981 tcgcccgtca acaccgtgaa gccgggagaa aacgcgagct ccaggcggtc cacgatcacg
    17041 aagtcgcgga tggaaaggct gcgcaacatg cttgatcgta agcggtgagc ggtaagcagt
    17101 aaacggttaa gtggctctgc gccgcggctt ttccgctcat tgcttactgc ttacggttta
    17161 ctctctattc cagttcaatt tctcccgcag catggcgtag tagctgtgcc cctccgggtg
    17221 caggaggctg atcgtgcgcg gatagcgccg caccacgacc cggtcgccct cgcgcaggtc
    17281 gcaccgcgtg tggctgtcga agtgtgcccg cggctcgggg tcatcacgca cgatgatttc
    17341 caccgtgcag ctgctgctga tcacgaccgg gcggttggac agcgtatggg gggacaccgg
    17401 caccagcgcc atcaccgaga gcgagggatg cacgatcggg ccgcccgagg acagcgcgta
    17461 ggcggtggag ccggtcgggg tggcgacgat caagccgtcc ccgcgctggt tgtagatgaa
    17521 ctggccgtcg atgcgcacct ccatctcgag catgctgccc ttgggcccct tgctgaccac
    17581 cgcgtcgttg aaggcaagcg cttcgaacac cttgttcgcg cctcggaaaa cctgcccctc
    17641 gagcagcatg cgctcctcgg ccacgaacct gccgtccagg atcgcgccga tagtggcgat
    17701 catggtttcc accgagatat cggtcaggaa gcccaggcgg ccctggttga ccccgaccag
    17761 cgccacgtca tggggggcga acgtgcgcgc gatgttgagc atggtgccgt cgccgccgat
    17821 gacgatggca aggtcgacct cgcggccgag ctcctcgagc gacagcaccg ggtatttgat
    17881 ctctttcttc acctgggagg cggtcaggcg atcgatcagg accttcacct tgcgccgctc
    17941 caggaagccg gcgagctcga gcagcggccc ggcgacttcc gggctcttgt acttgccgac
    18001 cagcgcgacg gtcttgaacg atgatttcat ggccgcattt taaccagaaa cggcgtgatg
    18061 agtgatgggt gatgagtaat gacaaattcc taagacaccc gcgggttaaa cggttcaaac
    18121 ccatcattca ccactcatca ctcatcgccg ctttttctgt agaataagtt catgcctccc
    18181 gactccccgg tgctgagcga acgcgcgcag attctcctga agacgctggt ggaacgctat
    18241 atcgcggaag gccagcccgt cggctcgcgc gcgttgtcga agtattccgg gctcgatctc
    18301 tcccccgcca gcatccgcaa cgtgatggcc gatctggagg agatgggctt catcgcgagc
    18361 ccccacacgt cggccgggcg ggtgcccacc gcccggggct accgcttctt cgtggacacc
    18421 ctgctgaccg tcaagccgct cgccacgatc gagatcaacc agctcgaggg gcagctgcac
    18481 gcggaaagca cgcagaagct cgtcgcctcc gcttcgcagc tgctgtccga tctcacccat
    18541 ttcgcggggg tggtgatgac acctcgccgc agcgccggct tccggcacat cgagtttctc
    18601 aggctctcgg acacgcgcgt cctgctgatc ctcgtcacgc ccgagggcga tgtgcagaac
    18661 cgcatcctgt tcacggtgaa ggcgtacacg ccctcggaac tcaccgaggc ggcgaacatt
    18721 ctcaaccagc actacgcggg gctcaccttc gaggaagtcc ggcggcgcat ccgcgcggag
    18781 ttgaggcagc tcaccgagga catgacgcgg ctcatgaccg cggcgctgga ggcgggcagc
    18841 caggccgcga gcgagtccgc ggaggaagtg gtgatatcgg gcgaaagcaa cctgctcgac
    18901 tcgcaggacc tctcctccaa catggcgaac ctgcgcaggc tcttcgagct gttcgaccgc
    18961 aagaccggcc tgctgcaact cctcgacagc tcgagccgcg cgcagggcgt gcagattttc
    19021 atcggcggcg agtcgggggt ggagccgctc gacgaatgca gcgtgatcac ggcttcctac
    19081 aaggtggaag ggcaggtggt cggcaccgtc ggcgtaatcg gccccacccg catggcctac
    19141 gagcgcgtca ttccgatcgt ggacatcacg gcgaagctgc tctccagcat gctcacgcag
    19201 cgctgagcaa aaaaacggga ctggggtcta gggactaggg actcgtaaat tcaagatcga
    19261 gccgcgagcc ccgagccccg atacccgatt ctcaatgtcc cgctacgctc ccgagccgcc
    19321 ctactcgcac ggcacattcc cccgcatcgg ggtcctcctg atccagctcg gcacgccgga
    19381 agcgccgacg gcggcggcgg tgcgcgccta tctcaaggac ttcctgtccg acccgcgggt
    19441 ga
//
LOCUS       DBNGWZ010000084         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1770439, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000084 DBNGWZ010000000
VERSION     DBNGWZ010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1770439"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..820
                     /locus_tag="AC16CX_02350"
     CDS             44..820
                     /locus_tag="AC16CX_02350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3011382.1"
                     /translation="MSLSVIIISRNNEDTMRRCLESVTWADEVVVVDSGSTDRTLEIC
                     REFTAKVHQTPDFPGHGPQKNRALDLATCDWLFSLDADEWVTPALRAELEAAMASPGD
                     KAAFMMPRRSSFCGRFMRHSGWWPDYVTRLFRRGAARFSEDHTHDRLIVDGKRGRLKQ
                     PVMHEAVTDLEQMLVKINMYSSTSAAMFHRQGRRASLATALLHGGWAFVRTYFLRAGF
                     LDGREGFMLAVINAENSYYRYLKLMLLAEREKPVTKRLSD"
     gene            901..>1788
                     /locus_tag="AC16CX_02355"
     CDS             901..>1788
                     /locus_tag="AC16CX_02355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR3011383.1"
                     /translation="MRNPPRTVLIIVTRRIGDVLLATPLMRSVKRAWPDSALDVLLFE
                     GTQGVIAANPDVRRILTVPERPGWLAHLAFIIRLLRRYDVALSTQHGDRPTLYAFLAG
                     RWRAGLLNATRKERWKQPFLHRWVPFDDWNTHTVLMNLALAGTLGIEPRPEVAVTWSA
                     DDAARVEALLGRAPAAPIAVLHPYPKFNYKKWHGAGWLETASWLAARGHRIVLSGGAD
                     PAEIAYVADLARDMPPGTLNLAGRLSLTEAGCLVSRAAVFVGPDTALTHMAAALGVPT
                     VALFGPTDPVKWGPWPKGHA"
BASE COUNT          298 a          603 c          567 g          320 t
ORIGIN      
        1 gttactcaat cacttaatca cttagtcact taatcactgg accatgagct tgtcggtcat
       61 catcatcagc cggaacaatg aggacacgat gcggcgctgc ctcgagtctg tgacgtgggc
      121 cgacgaggtc gtggtggtgg attccggcag caccgaccgc acgctggaga tctgccgcga
      181 gttcaccgcc aaagtccatc agacgccgga tttccctggg cacggcccgc aaaaaaaccg
      241 ggcgctcgac ctcgcgacat gcgattggct gttttcgctg gacgccgatg aatgggtgac
      301 gccggcgttg cgcgccgagc tcgaggccgc gatggcttcg cccggcgaca aggcagcatt
      361 catgatgccg aggcgctcga gtttctgcgg gcgcttcatg cgccattcgg gatggtggcc
      421 ggattacgtg acgcggctgt tccgccgcgg cgcggcgcgc ttctccgagg accacaccca
      481 tgaccggctg atcgtcgacg gtaagcgcgg ccggctcaag caaccggtca tgcacgaggc
      541 cgtcaccgac ctcgagcaga tgctggtcaa aatcaacatg tactcctcga ccagcgccgc
      601 gatgttccac cggcagggac gccgcgcctc gctcgccacc gcgctgctgc acggcgggtg
      661 ggcgttcgtg cgcacttact tcctgcgcgc gggttttctc gacggtcgcg aaggtttcat
      721 gctggccgtg atcaacgccg agaattccta ctaccgctac ttgaagctca tgcttctcgc
      781 ggagcgcgag aagccagtga ctaagcgatt aagtgactga gtgattgagc aagagccaag
      841 accgaacgga tcctgccacc acttaatcac ctaatcactt aatcactcaa tcactcgtcc
      901 gtgaggaacc cgccacgaac cgttctcatc atcgtgaccc ggcgcatcgg cgacgtgctg
      961 ctcgcgacgc ccctgatgcg ctcggtcaag cgggcgtggc cggacagcgc actcgacgtg
     1021 ctgctgttcg aggggacgca gggcgtgatc gccgcaaatc cggacgtcag gcgcatcctc
     1081 accgtgcccg agcggccggg ctggctcgcg catctcgcgt tcattatccg attgctgcgg
     1141 cgttacgatg tcgcgctgtc cacgcaacat ggagaccggc ccacgctcta cgcgtttctc
     1201 gcaggccgct ggcgcgccgg gctgctcaat gcgacgcgca aggagcgctg gaagcagccg
     1261 tttctccacc gctgggtgcc gttcgacgac tggaacaccc ataccgtgct catgaacctt
     1321 gcgctcgccg gcacgctggg aatcgagccg cgcccggagg tcgccgtcac atggagcgcg
     1381 gacgacgcgg ctcgggtgga ggcgcttctc ggccgcgcgc ccgcggcgcc gatcgcggtg
     1441 ctgcacccct atcccaagtt caactacaag aagtggcacg gcgcgggttg gctcgaaacg
     1501 gcgagctggc tcgcggcgcg cggccatcgc atcgtgcttt ccggtggtgc cgatccggcc
     1561 gagatcgcct acgtcgccga tctcgcgcgc gacatgccgc ccggtaccct gaacctcgcc
     1621 ggtcggctgt cgctcaccga ggcgggatgc ctggtctccc gcgccgccgt ctttgtcggg
     1681 ccggacactg cgctcacgca catggcggcg gcgctcggcg tcccgaccgt tgcgctcttt
     1741 ggtccgaccg accccgtcaa gtgggggccg tggccgaagg gccacgct
//
LOCUS       DBNGWZ010000085        67912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_7635, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000085 DBNGWZ010000000
VERSION     DBNGWZ010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 67912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 67912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_7635"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1802
                     /gene="glmS"
                     /locus_tag="AC16CX_02360"
     CDS             9..1802
                     /gene="glmS"
                     /locus_tag="AC16CX_02360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3011384.1"
                     /translation="MIEGIKRLEYRGYDSTGIAYVKGSRLERLRSTGRVAHLDELARG
                     QGVLASTGLSHTRWATHGAPTEANAHPHFSARGGLEIGVVHNGIVENHEPIRARMKTL
                     GYGFSSETDTEVMAHHVHSLVAGGLSLFEAVKAASREWQGAYAVGFISSREPDVVVGA
                     RRGSPLLVGLGQGENFLASDASALVARTREVIYLEEGDVVEVRPQGVTICDGDGRPAQ
                     RAVTRSELSADAVEKGPYAHYMQKEIFEQPGAVANTLEMVANAQSLVPNLFGAEAREV
                     FSRTNQLLLLACGTSYHAGLVARYWLESVAGLPATVEIASEFRYRDPVAQPGTLVVTI
                     SQSGETADTIAALKHAQARGLADTLTVCNVPESALMRASRLRFLTRAGPEIGVASTKA
                     FTTQLAALFVLTLVLAKLKQRLAPEREQELIAQLRHLPRAMEAVLSVEPQVRDWARRF
                     QPKHHALFLGRGVHFPIAMEGALKLKEISYVHAEAYAAGELKHGPLALVDADMPVVTV
                     APNDQLLEKLKSNMQEVRARGGELYVFADRDARVAAAEGVHVIRLVDHAGALSPILHV
                     IPLQLLAYHVAVLRGTDVDKPRNLAKSVTVE"
     gene            complement(1976..2749)
                     /locus_tag="AC16CX_02365"
     CDS             complement(1976..2749)
                     /locus_tag="AC16CX_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011886170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3011385.1"
                     /translation="MRSAQRIIITGASSGIGAALARRYSASGATLGLIARRKPELERL
                     AAGFASRCEIYPLDVRDAAALAAAARHFIKRHGCPDVVIANAGVSVGTLTEFAEDARA
                     FEEVIDTNVIGMVNTFRPFVAGMRERDGGALAGIASVAGYRGLPGAAAYSASKAAAIA
                     YLESLRVELRGSGVKVITICPGYIATPMTEKNPYPMPFLMGADEAAAKIASLIARGKA
                     YSVIPWQMALIARILHVLPNWAYDRLVAGARRKPRRWAG"
     gene            complement(2881..3540)
                     /locus_tag="AC16CX_02370"
     CDS             complement(2881..3540)
                     /locus_tag="AC16CX_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR3011386.1"
                     /translation="MGGNLPFLLARGGLTLLALIGLAGTAPAQEIRARQNIEYRLIAS
                     QPVETGDRIEVIDFFWYGCPYCNELQPALEEWLKRKPADVALRRIPVILKDTWAPHAR
                     IYYTLELLGEVERLHQKVYYSYHVEELHMSKPDVIEQWAVKNGIDRRKWLDAYSSPEV
                     DAKVARAQQLTQRYDVQGTPSVVVDGRYLTSSSMTPAVRGVVPVLEDLIRLARQKRSE
                     K"
     gene            complement(3554..4234)
                     /locus_tag="AC16CX_02375"
     CDS             complement(3554..4234)
                     /locus_tag="AC16CX_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HIR3011387.1"
                     /translation="MSFIPSCLRLAVWLLALTVSANASAQLVAGKDYILINPPQPTNS
                     GKKVEVVEFFWYACPHCNSLQPSLHAWLKRKPADVEFRRVPAAFQESWLQLARTYYAL
                     EAMGLVERLHRDLFAAIHEQRALNPKALLSNPSPLFDWIASKGADRQKFIDTYNSFAV
                     NTRTQRTMQVTESYDIRFTPTLVVNGRFITAPSLGLRDGENVNYERYFQVLDQVIAMA
                     RKQRAGKK"
     gene            complement(4266..4841)
                     /locus_tag="AC16CX_02380"
     CDS             complement(4266..4841)
                     /locus_tag="AC16CX_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3011388.1"
                     /translation="MSKDYKRPEPKSRGGGSSLIIGILIGLVLGLGIALAVAWYINKM
                     PSPFLSRTPPAKGEPAKGAQAPARSEEKTARAHEAKPRFDFYKILPGTEEPATDQQLR
                     ESQKKSTAAAKETFFLQAGAFQSAPDADNLKARLALLGIEAKIQTTTLPDRGVWHRVR
                     VGPYTSVEELNRTRDTLKQNGVETALIKVRE"
     gene            complement(4851..6590)
                     /gene="argS"
                     /locus_tag="AC16CX_02385"
     CDS             complement(4851..6590)
                     /gene="argS"
                     /locus_tag="AC16CX_02385"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR3011389.1"
                     /translation="MAPIDDARALLAGLLAEALRKVAPDRTDAAIVLERPKRAGHGDY
                     ACNVALQLAGTLRRAPREIASQLVAALPASPHLEKAEVAGAGFINLFLTREFKQQAVN
                     RILQAGTAYGTIAPAQKKRVQVEFVSANPTGPLHVGHGRGAAYGASLANVLAAAGYDV
                     TREFYVNDAGRQMDILALSTWLRYLEACGVTVDFPPNAYQGEYVRRMALNIRAQHGDE
                     FRRGIAVDVFTTLTAEPDEETRLDALIGLAKEKLGADYAYIHDFALNEQLEDCRSDLA
                     EFGVTFDRWFSERSLYDSGLVERAISALEQRGHVYRREGASWFRSSAFGDEKDRVVRR
                     ENGQYTYFASDIAYHLNKFERGFETVIDVWGADHHGYMPRVKGALAALGLNPEQLTIA
                     LVQFAVLYRNGQRVSMSTRSGEFVTLRELRREVGNDAARFFYVLRKSDQHLDFDLDLA
                     KSQSNENPVYYVQYAHARVCSVLEQWGGGVRKLVAADVSALVTEHELALLARLMEYPE
                     AVEIAAHDLSPHLIAFYLRELAGEFHSYYNATRILVEEEDIRLARLVLAAAVRQVLRN
                     GLALLGVSAPDKM"
     gene            complement(6778..8967)
                     /locus_tag="AC16CX_02390"
     CDS             complement(6778..8967)
                     /locus_tag="AC16CX_02390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR3011390.1"
                     /translation="MGMPIIRVALDVPVDTLFDYRAPDATAADIGRRVVVPFGNRTAI
                     GVIVEVSSASGVPVERLKNALRVLRDLSPLAAEDLRLMRFAADYYHHPLGAVVMGTLP
                     TRLRRVAGRSQPRERGAYLLTAAGAALDASALPLRARAQRRVLERLHQGPLGHAEART
                     LVPNARSLLKQFLSRGWITLEEDRKTDVPPLPFTVASRELTAEQARAVNEICAGLGKY
                     RPFLLLGVTGSGKTEVYLHAIAAALKTGRQALLLVPEIALTPQLEALVGHRFPGTRTA
                     SLHSGLADSERLENWRAAREGRARVVLGTRLAVFAPLPELGLIVVDEEQDGSFKQAEG
                     FRYSARDLAVARARQHGVPVVLASATPALETYHNAVTGRYSLLTLSSRIGAPPPRITC
                     IDTRGEKLVDGLSRPLLAALRERLAGGEQSLVFINRRGYAPVLMCRACGWISGCGRCS
                     AQLVLHFTERRLRCHHCGHVSPAPSACPGCGNPDLAPIGQGTQRVEQALARHFPQART
                     LRIDRDTTSRRHAWQTMRQRIQDREVDILVGTQILAKGHDFPHLNLVGVLNADSRLYS
                     NDFRAAEQLYALLTQVAGRSGRGGARGEVLIQTEFPDHPLYAALRQQDYRAFADSALA
                     ERRQAGFPPFVYQAMLRAEAPRLATALEFLNEAARVGKGLDARVTAYDPVPAAMPRRA
                     GRERAQLLVQSASRERLRDFLGAWHGVLARVRVTRARWALDVDPIDF"
     gene            complement(9310..10371)
                     /gene="hemE"
                     /locus_tag="AC16CX_02395"
     CDS             complement(9310..10371)
                     /gene="hemE"
                     /locus_tag="AC16CX_02395"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3011391.1"
                     /translation="MTKLKNDTLIRALFRQPTPYTPIWLMRQAGRYLPEYNEARARAG
                     SFLALCKNPALATEVTLQPLARFRLDAAIVFSDILTIPDAMGLGLNFAEGEGPRFERP
                     LREEREIRRLVAPEPGEQLRYVLDTVRQVRRALAAEVPLIGFSGSPFTLACYMVEGGA
                     SADFRTVKTMLYTRPDLLRCILEVNRRAVTAYLNAQIESGAQAVMIFDTWGGVLAAAE
                     YREFSLAYMQRVLDGVKREHEGERIPTILFTKGGAAWLEALAASGCDAVGLDWTVDIG
                     AARDLVGQRVALQGNLDPAALLAGPEAARRGAAAILAAFGPGAGHVFNLGHGISQFTS
                     PDCVQALVEAVRELSPAYH"
     gene            complement(10454..11917)
                     /locus_tag="AC16CX_02400"
     CDS             complement(10454..11917)
                     /locus_tag="AC16CX_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR3011392.1"
                     /translation="MGKVTGFLEFERVEEAYEAKDRRKKHFREFIPPLTDEQAKIQGA
                     RCMDCGIPFCMSGCPINNIIPDWNDLVFRQDWKAAIDTLHSTNNFPEFTGRVCPAPCE
                     EACVLRINEDPVGIKSIEHAIIDKAWERGWVKPQPAAQRTGKKVVVVGSGPAGLACAQ
                     QLARAGHDVVVFEKNDRIGGLLRYGIPDFKLEKWQIDRRMEQMAAEGVSFRVGVYVGN
                     QRPGKGVPDWATEMIVPEKLLPEFDAVVLAGGAEQSRDLPIPGRDLDGVHFAMEFLPL
                     QNKRVAGDTGVPDLRATGKHVVIIGGGDTGSDCVGTSNRHEARSVTQFELLPMPPDVE
                     RNPVWPYWPTRLRTSSSHEEGVMRDWSVATKEFIGEGGKVKALRGVRLEWRDGRMSEI
                     AGSEFTLPADLVLLAMGFVSPVQSILDAFGVEKDARGNANATTDGAGCYATSMPKVFA
                     AGDMRRGQSLVVWAIREGRQCARAVDEFLMGSSELPR"
     gene            complement(11981..16654)
                     /locus_tag="AC16CX_02405"
     CDS             complement(11981..16654)
                     /locus_tag="AC16CX_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3011393.1"
                     /translation="MNELWNQGGLPSQRGLYHPAHEHDACGVGFVAHIKGKKSHAIIE
                     QGLTVLRNLTHRGAVGWDPKLSDGAGCLIQIPDRFFREEMAKRAVKLPPVGQYGVGMV
                     FLPRDPASRLACEYEIERAIRDEGQVLLGWRDVPVDNTDLAEPAQRIEPVIRQVFIGR
                     GRGITVTDALERKLYIIRKSAGHAIQALKLVHGKEFYVPSMSARTVCYKGMLLAYQVG
                     EYYLDLKDARVESALALVHQRFSTNTFPSWDLAHPFRMICHNGEINTLRGNINWIRAR
                     QGAISSPILGRDLDKVWPLIYDGQSDSASFDNALELLVMGGYSIAHAMMMMIPEAWEN
                     HTLMDPARRAFYEYHAAMMEPWDGPASIAFTDGRQIGATLDRNGLRPSRYIVTSDDLV
                     IMGSECGCLPVPEEKIVKKWRLQPGKMFLVDLEKGRIIDDKELKDTLASARPYAEWIE
                     RIRVKLDELPAPSEKREASKERLLDRQQAFAYTQEDVKFQLAPMATNGEEPVGSMGND
                     AALAVLSRKNKTLFHYFKQLFAQVTNPAIDPIREELVTSLVSFIGPKPNLLGIDEMNP
                     PLRLEVSQPVLDFEQMETIRYIEKYTDGKFRSFELDIAYPVAWGKQAVEARLASLAAQ
                     AEDAVRSGYSILIISDRKMDRERVAIPALLALSAIHQHLVNKGLRTSTGLVVETGSAR
                     EVHHFALLGGFGAEAVHPYLAFETLIELADKGLLGPGIDAGKACKNFIKAVGKGLKKV
                     MSKMGISTYMSYTGAQIFEAVGLARSLVDKYFSGTTSSVEGIGVFEVAEEAIRIHRAA
                     FDEDDPVLRDALDVGGEYAWRVRGEEHMWTPDAIAKLQHSTRNRSYSTYKEYAALIND
                     QSKRYLTFRGLFEFRFERCKPLPIEEVEPAAEIVKRFSTGAMSLGSISTEAHTVLAIA
                     MNRIGGKSNTGEGGEDRKRYAKVAAGETLLSRLGRERVEVDIPLEEGDSLKSKIKQVA
                     SGRFGVTAEYLASSEMMQIKIAQGAKPGEGGQLPGRKVSEYVAEIRYSTPGVELISPP
                     PHHDIYSIEDIAQLIHDLKNANPEAAVSVKLVSEVGVGTVAAGVSKAKSDHVTIAGHD
                     GGTGASPWSSIMHAGTPWELGLAETQQTLVLNRLRGRIAVQVDGQMKTGRDVVIGALL
                     GADEFGFSTAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRRKFEGKPEHVVNYFFFIA
                     EEARELMAKLGIRRFNDLIGRSDLLDVQKGIEHWKAKGLDFSKIFQVPQMPAEVARYH
                     REQQDHGLEKVLDRRLIELARPALEHGEKVTIETPIRNINRTVGTMLSWEIARRYGHE
                     GLPDDTIRIRLAGSAGQSFGAFLAKGVTLDLIGDTNDYCGKGLSGGRISVQPSPKFRG
                     EPTENIITGNVVLYGAICGEAYFRGVAGERFAVRNSGAQAVVEGVGDHGCEYMTGGSV
                     VVLGVSGRNFAAGMSGGIAYVLDVDGEFSRRCNTSMVALEPLLSEAEQDAKLVRDLWH
                     GGVADELIVKRLIENHARYTGSAQAKCIVERWSEFRPKFVKVFPHEYRRALGELAAKQ
                     RRLVA"
     gene            complement(16793..17920)
                     /locus_tag="AC16CX_02410"
     CDS             complement(16793..17920)
                     /locus_tag="AC16CX_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR3011394.1"
                     /translation="MPDRELAPYAARPEHSRGRRHREPAPRGRSEYQRDRDRIIHSTA
                     FRRLEYKTQVFINHEGDLFRTRLTHSLEVAQIARSVARSLRLDEDLTEAVALAHDLGH
                     TPFGHTGQDALNACMRPYGGFEHNLQSLRVVDILEQRYAAFDGLNLTFEAREGVLKHC
                     SLRNARTLGDVGERFVKKRCPSLEAQIANLADEIAYNNHDVDDGLRSGLLSLDQLAGV
                     RIVARHLKAVLREHPKVTGRRLVHETVRRMIDTLVTDLVRASAENIRRHAPGSIEEVR
                     LAPPLITFSPRIREESRELKHFLRENLYRHYRVVRMSAKARRIVTELFGVFLSDARLL
                     PPEFQARALNDHSRAIADYIAGMTDRYAILEHRRLFSIEET"
     gene            complement(17913..19004)
                     /gene="aroB"
                     /locus_tag="AC16CX_02415"
     CDS             complement(17913..19004)
                     /gene="aroB"
                     /locus_tag="AC16CX_02415"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR3011395.1"
                     /translation="MQTLNVELGERSYPIHIGAGLLTRAALIAGRLRHRKAAIVTNST
                     VAPLYLMPLTRALETERVEVVSITLPDGEAYKNWETLNRIFDALLEHRCERSTALIAL
                     GGGVIGDLAGFAAAIYQRGVPYLQVPTTLLAQVDSAVGGKTAINHPLGKNMIGAFYQP
                     LAVISDTDTLNTLPARELSAGLAEVIKYGLIRDREFFDWLEANMARLVRREPEALAYA
                     IERSCANKAEVVALDERESGPRALLNFGHTFGHAIEAGTGYGAWLHGEAVASGMTLAA
                     RLSHRLGLIGKEEAGRVASILAVAGLPVEAPDLGLERYLELMGHDKKIEGGRIRFVLL
                     KRIGEAIVSDQVPGAALAEVLRGATRAHA"
     gene            complement(19027..19626)
                     /locus_tag="AC16CX_02420"
     CDS             complement(19027..19626)
                     /locus_tag="AC16CX_02420"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3011396.1"
                     /translation="MGDDAQKTAAGQGRVPGNIFLVGLMGAGKTSVGKLLARRLGKTF
                     YDCDHEIERATGVKVAVIFEIEGEAGFRAREAKILAELVRRREIVLATGGGAVLSADN
                     RKLLAENGVVVYLRANSADLWSRTRHDKNRPLLRTADPLARLQQLFDERDALYRSVAD
                     IVVDTGSQSLGSLTHRLEHQLLRHHADASRQENAPLRTG"
     gene            complement(19737..21863)
                     /locus_tag="AC16CX_02425"
     CDS             complement(19737..21863)
                     /locus_tag="AC16CX_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR3011397.1"
                     /translation="MKLRAFSIFSTILPGMLGLLLAVASGAARAQANTIEAINVSPAT
                     GGKVVVRVTLKQAPASPPAGFTVNNPPRIAFDFPNTTSALGRSTQEVGEGDLRSINVV
                     QAGDRTRLVLNLTRATSYDTQIDGRALLITLQGAAAAEGPASVTTHFAEAQPGDRRHA
                     LRDIDFRRGSAGEGRVVIDLSDNAVGIDLKLQGKTIVVDFINTALPKNLQRRLDVTDF
                     GTAVQTIDAFTQGSNTRVTIEPQGAWEHTAYQSDTRFIVEVKQQTQDQDRIARGGGYT
                     GEKLSLNFQNVEVRAVLQVIADFTGLNIITSDTVGGNLTLRLKDVPWDQALDIILQSK
                     GLDMRKTGNVVWIAPRDELATREKLALEARQQITDLEPTRTESFQLNYQKADAFQKLL
                     SDPNQKILSKRGSAVVDARTNTIFVQDTPSRLEEVRKLIRKVDVPVRQVMIESRIVEA
                     TDNFSRNLGVRLGYREEADGSKITSSPSSKAPRVTVGGSATTLAPLAGTAAVAPFSSG
                     NLNVNLPVPGLGGASPGVFSVILFNQSLTKFLDLELTALQADGRGKIISSPRVITADQ
                     VEATIEQGTEIPYQQATSSGATSVSFKKATLSLKVKPQVTPDDNVIMNLNIHKDSVGQ
                     NTLSGPSIDTKQIVTEVLVENGGTVVIGGIYTQTETSTTNKIPVLGDLPYVGFLFKQN
                     LKSDNRNELLIFITPRIIKDRLTLRQ"
     gene            complement(21876..22397)
                     /locus_tag="AC16CX_02430"
     CDS             complement(21876..22397)
                     /locus_tag="AC16CX_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016254.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HIR3011398.1"
                     /translation="MTRYILAISLSCLALAACSGEQHSDLRQFVKDSDSMPRGRIPPL
                     PEVKPYEPFEYAAFDLVDPFVPRKIEAPKRPPSPTIDPKVFDPNRRKGPLEAFPLENL
                     KMVGTLQQKKEFFALIKTPDNNLYRVQTGDFMGQNFGRITDISESAVKLKELIQDSGN
                     EWKEEERTLLLQE"
     gene            complement(22394..23038)
                     /locus_tag="AC16CX_02435"
     CDS             complement(22394..23038)
                     /locus_tag="AC16CX_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR3011399.1"
                     /translation="MAMTLDDLKRLDPKKIGSWPILPKLVVLLLVLVVIVGVSYWFDW
                     DDQINRIKAEAQKEEGLRKTFLTKKSEAINLPAYQKQLEDIEKQFGALLKQLPGKSEM
                     DALLTDINQAGLGRGLQFELFKPAASETRRDFYAELPITIKITGSYHDIGAFASDISK
                     LSRIVTLNDVGLTPGGAKGGGLVLDATARTFRYLDEDEIAQQRKKAAPAAKGKK"
     gene            complement(23045..23638)
                     /locus_tag="AC16CX_02440"
     CDS             complement(23045..23638)
                     /locus_tag="AC16CX_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR3011400.1"
                     /translation="MIRINLLPHREIRRKQQQQQFFILLGSVVVIGAVIWFAVHTYLE
                     DQFNNQVSRNKYLQSEIEKLDKQIAEIQKLKDQTAALLARKRVVETLQATRSEVVHLL
                     DQLVRQLPDGVYLKSIKQVGTRITISGFTQSQARVSTLMRNLESSPRLESPGLIEIKA
                     AQDRGQRVNEFIMNVNITRAKVEDAAKKPAPKPAAKG"
     gene            complement(23635..24741)
                     /locus_tag="AC16CX_02445"
     CDS             complement(23635..24741)
                     /locus_tag="AC16CX_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HIR3011401.1"
                     /translation="MLKGKFSLNLDAIEGLFKAKMPPLIGADISSSAIKMVEIEGAGK
                     GMYRVERYAIEPLPKDSLVEGNINNLDAVSDALKRCHKRLGSNIKNLALALPNAAVIS
                     KKILVPGGQTEEDLELQVETEANQYIPFALEEVNLDFQVLGPAPNSAEDVEVLIAASR
                     KDKIIDRVAAAEAAGLKAVVMDVDVFAAQTAFELIETQFSEKGKDQNIAIVDVGATIM
                     AVNVLRNDQSIYMREQPFGGNQLTQEIQNRFGLSPEEAEAAKRAGGLPENYESDVLQP
                     FMDMLGLEVARALQFFFTSTQHNQIHHIVLSGGCAAIPGVDEAVSKRVQVNAMVANPF
                     VNMALSSKIRAKSLTQDAPSLMVACGLAMRRFDP"
     gene            25044..27302
                     /locus_tag="AC16CX_02450"
     CDS             25044..27302
                     /locus_tag="AC16CX_02450"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR3011402.1"
                     /translation="MAAFAAVIVYPTLPSLEMLTDYQPKIPLRVYSADGKLIGEFGEE
                     RRSVVKIQNVPEPMIRAILAAEDERFYQHHGVDYVGVARAALSNFMSGGVRQGASTIT
                     MQVARNFFLSKEKTLTRKFNEMLLAFKIEANLSKDQILELYINQIYLGQRAYGFAAAA
                     QIYFGKQLSELTLAESALLAGLPKAPSRYNPLANPKRAKLRQQYVLRRMRELQFISEE
                     ELASADRQPVLVKKEINDYPVRAEHFAEMVRQAIYERYQEDTYTKGIRVYTTLLTSHQ
                     EAAYAAMRRGVQEYDRRHGYRGAEGYVELPAKLTDETLEDALQETTDSDDVYAVVVIE
                     AGPKLVRAYRKGGEWVEIKEEGLKFAARMLGEKASPGQRIRRGAIIRVQKDEKGRWQI
                     TQLPAVEASVIAVDRRDGAIRALVGGFDFSHNQYNHVTQALRQPGSSFKPFIYSAALE
                     KGFTTATVINDAPLTFTAAQTGSEPWEPKNYDSKFEGPMRLRTALVKSKNLVSVRILQ
                     AISPQYTQDYIARFGFDPKLHPPYLTMALGSGNVTPMQMATGYAVFANGGYRVAPYFI
                     ERVEDARGNVLMSAKPLAAGAGAERVIDARNAFIMSSIMRDVVRMGTGARAMKLGRVD
                     LAGKTGTTNEFVDAWFCGYGGELVAVAWIGFDTPQTLGRNETGAQAALPIWMGFMGAA
                     LKGVPEQPLTPPAGIVAMRVNPDTGLRVADAQGGIVDFFYSEFLPPEHETLIGGVIGG
                     DRPREEVRNQLF"
     gene            27310..27912
                     /locus_tag="AC16CX_02455"
     CDS             27310..27912
                     /locus_tag="AC16CX_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011403.1"
                     /translation="MPRETGRNDRRSRIAHFAARLMAEDGIEDYALAKRKAARQAGTP
                     DTRELPTNEEIDAALRTYQQIYHKEEHRNRLRELRGRALRAMRELAQFNPYLTGSVLS
                     GNAGKYADINLQLFTDNAKAVELYLIDRRIPYRVAQSRLYCGAEPLTAPVYTVDDDGT
                     EIELTVLSTRELRGPLRTSPEGKNIEHAKLLVVEQLLAQT"
     gene            complement(27913..28920)
                     /gene="hemB"
                     /locus_tag="AC16CX_02460"
     CDS             complement(27913..28920)
                     /gene="hemB"
                     /locus_tag="AC16CX_02460"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3011404.1"
                     /translation="MRVYGGFPRRRMRRMRRDEFTRRLQREHRLTTDDLIYPVFVIEG
                     RNRTEAVPSLPGIRRYTVDRLLAHAEQCARLGIPALVVFPMIASELKTRDGREATNAK
                     GLVPRTVATLKKRFPELGVITDAALDPYTTHGQDGVIDRDGYVLNDETIAVLEKQALV
                     CAAAGVDVVAPSDMMDGRIGAIRAALDRQQFIDTRILAYSAKYASGFYGPFRDAVGSA
                     KNLGRGNKFTYQMDYANSNEALWEVGLDLEEGADMVMVKPGLPYLDIVRRVKEEFKAP
                     TFVYHVSGEYSMLKAAAARGWLDERPCVMEVLTAFKRAGADGILTYYAFDVAGWLKAG
                     G"
     gene            29273..29908
                     /locus_tag="AC16CX_02465"
     CDS             29273..29908
                     /locus_tag="AC16CX_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011405.1"
                     /translation="MSFRWPVMLLAATLISPAATAQGPAKSDPSKAQPIATQVCGACH
                     AVDGNSTQAANPVLAGQHADYTLKQLANFKPLDGKPAERPHAVMAGMVANLSADDMRN
                     LAAYFEIQKPKPRAARDPELVKLGRAIYRGGILSKNVAACTACHGPNGSGVPAQFPRL
                     AGQFAEYTAAQLKAFRSGERANDPNRAMRALAEKLSDKEIAAVAEYIAGLR"
     gene            complement(29918..31198)
                     /gene="hemL"
                     /locus_tag="AC16CX_02470"
     CDS             complement(29918..31198)
                     /gene="hemL"
                     /locus_tag="AC16CX_02470"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006410509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3011406.1"
                     /translation="MKRSQKLFQHAQLHIPAGVNSPVRAFHSVGGTPLFIQRGKGAQV
                     WDADGRAYIDYVGSWGPLILGHAHPDVVKAVQQAAERGLSFGAPTEQEIEMAELLCRL
                     VPSLEQVRLTSSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADALLVKAGSGALTF
                     GQPSSAGVPEPAAAHTLVLEYNDEIALERAFDREGAEIAAVIVEPVAGNMNLVAPHPG
                     FLELLRKLCTRHGAVLIFDEVMTGFRVALGGAQALYGVKPDLTTLGKVIGGGMPVGAF
                     GGRRDIMQKIAPLGPVYQAGTLSGNPVAVAAGLATLKLIQASGFFERLTAITRSLCEG
                     LTRCAQVHGMPFSAASVGGMFGVYFRETPPTSYAEMMQCDREAFGRFFHAMLAEGIYF
                     APSAYEAGFVSSAHGAAEIDKTIGAARKVFATIS"
     gene            complement(31195..31839)
                     /gene="thiE"
                     /locus_tag="AC16CX_02475"
     CDS             complement(31195..31839)
                     /gene="thiE"
                     /locus_tag="AC16CX_02475"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR3011407.1"
                     /translation="MKRGISGLYAVTPDTDDTSDLLARVEAALAGGARLLQYRNKTAS
                     AVLRLTQGRVLLALCRKYRVPLIINDHLDLTLALDADGLHLGSADGSLPAARARLGDR
                     KILGASCYNRMQSALDAEHAGVDYVAFGSFFPSAVKPGAVQAPLALLQESKQRLSVPV
                     VAIGGITLDNAPQLIAAGADGVAVISALFGAPDVRAAARGFSALYREIDTIRSK"
     gene            complement(31843..32682)
                     /gene="thiD"
                     /locus_tag="AC16CX_02480"
     CDS             complement(31843..32682)
                     /gene="thiD"
                     /locus_tag="AC16CX_02480"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR3011408.1"
                     /translation="MPDLPPIVLVFAATDPSGGAGLQADIMTLSSMGCHPLSVVTAVT
                     IQDTMGVDDVSPIDAEWVADQARCVLEDMPVAAFKIGLLGNIEQITAIAEVVSDYPEV
                     PLVLDPVLASGRGDELVTDEMVSALRELLIPQTTILTPNSLEARRLAAEEAEEGDGLD
                     LAECARRIIASGCEYVLVTGTHENTTQVVNTLYGQNGVVRSDTWQRLPGSYHGSGCTL
                     ASAIAATIANGLEVSDAVKDAQEYTWQTLKAAFRPGMGQHIPDRLFWAREEEQAGGGS
                     SEG"
     gene            32805..32957
                     /locus_tag="AC16CX_02485"
     CDS             32805..32957
                     /locus_tag="AC16CX_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0043448 - alkane catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HIR3011409.1"
                     /translation="MCLICGFIYDEAAGLPEEGIAPGTRWEDVPPNWTCPECGARKDD
                     FEMVEI"
     gene            33056..33445
                     /gene="gloA"
                     /locus_tag="AC16CX_02490"
     CDS             33056..33445
                     /gene="gloA"
                     /locus_tag="AC16CX_02490"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HIR3011410.1"
                     /translation="MRILHTMIRVGDLQRSIDFYTQVLGMRLLRKTDYPGGRFTNAFV
                     GYADESKTAVLELTHNWDTRSYDLGTGYGHVAIEVEDAHQACADVKQRGGKVTREAGP
                     MKHGTTVIAFVEDPDGYKIELIQRKTA"
     gene            complement(33442..34230)
                     /gene="rsmA"
                     /locus_tag="AC16CX_02495"
     CDS             complement(33442..34230)
                     /gene="rsmA"
                     /locus_tag="AC16CX_02495"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3011411.1"
                     /translation="MDGLMRHVPRRRFGQNFLTDESVIAAIVAAIAPQPRDLMVEIGP
                     GLGALTRPLASRLDHLHVIEIDRDITARLRAAFPVARVTVHEGDALEFDFSALGSLLR
                     VVGNLPYYISTPLLFHLARHAGSIRDIHVMLQKEVVERMVATPSSSDYGRLSVMLQYR
                     FGMEHVLNVPADAFRPAPKVESAVVRMTPFARPPHLACDEALFGKIVAAAFARRRKTL
                     RNALKGYFGSRDFERLGVDAGLRAQDLRVEDFVRLADAAAGTAA"
     gene            complement(34241..35260)
                     /gene="pdxA"
                     /locus_tag="AC16CX_02500"
     CDS             complement(34241..35260)
                     /gene="pdxA"
                     /locus_tag="AC16CX_02500"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3011412.1"
                     /translation="MQHSKFKIPLIAVTAGEPAGIGPDLCVQLARRRLPARIVVVADR
                     GLLMSRARRLGVALKLLDFPTAAAAKPGAGALRVRHVPLARPAVPGRLDPANSRYVLH
                     TLETAADGCREGIFDAMVTAPLQKSVILEAGIAFTGHTEYLAAHTGAEHVVMMLVGGG
                     MRVALATTHLALKDVARNITREGLERTLRVLRHDLIERFSVPRPRILVAGLNPHAGES
                     GFLGREETETIVPVLEKLRREGFDLVGPLPADTLFQPSRLKDCDCVLAMYHDQGLPVL
                     KYASFGAGVNVTLGLPIIRTSVDHGTALDLAGTGSAEIGSLVEAIRLAAALATGGRGR
                     KRRAA"
     gene            complement(35332..36648)
                     /locus_tag="AC16CX_02505"
     CDS             complement(35332..36648)
                     /locus_tag="AC16CX_02505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3011413.1"
                     /translation="MIGFGGSSSRYAALAFAIALAPAAWAQQPRTVIVLDRIVAVVNN
                     EVITRGDLDEHTRHAAAQLKQQGTPPPPREALERQTLERLIINRVQLQLAKETGLRVD
                     DTELDRAIQRIAQENRLTLEGLRAALEKDGVSFSKFREDIRNEIILARLREREVDNRI
                     VVTESEIDNFINTQQAQTERGEEFNLSHILVTVPENASPEQIQARRSRAEQALEQLRS
                     GADFRQVAASFSDAPDALQGGTMGWRDAARLPALFLDALKTMQPGAASPLLRSANGFH
                     ILRLNDRRGSTVPVIIRQTRARHILIKTNELVPESEARRRLVSLKERLDNKADFAELA
                     RQYSEDASNTKGGDLGWLSPGDTVPEFERAMNALNPGGISEPVQTPFGWHLIQVLERR
                     NEDMSRERLRLEARRALRARKSDESYQEWLRQQRDLAYIEYRLEER"
     gene            complement(36665..38950)
                     /locus_tag="AC16CX_02510"
     CDS             complement(36665..38950)
                     /locus_tag="AC16CX_02510"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR3011414.1"
                     /translation="MHKPEITRISFAVLCALPLAAGAQQGLKLRPQPTLILIPPSLKE
                     EVPLFLEADRLQGHNEKETEAEGNVRLRKRGEAVYADRLRYDKPLDEVTAEGNVRMER
                     GADVVEGARLKYNLGTERGFMESSRYTLHKSPSLGGERQLFRETDARGTAERILFEGS
                     NRYRAQRSEYTACAPGNDDWYLRAGDLAIDKDRDIGVAHDASIVFMGMPIFYSPYLSF
                     SLHQGRKSGFLAPHYGSTDKGGAELTVPYYWNIAPNRDATFSPRVMTKRGVLVNNEFR
                     YLEPAYFGEARADVLPDDRERSGERRWAYNIRHNQTMPGGWFGTLNLQGASDGNYFTD
                     LSTQIALTSQTALPREGSIGRGGTWGSAGTYGFNALFQRWQALQTDPLVPVIPPYARL
                     PQLTLNATREDIAYSDFDFYGHYVGFDHPTNVTGSRTLAYPSMSLPLQTAYAHVTPKL
                     GLNVTHYSIDPNAAAVTDRSRTLPIFTTDSGVVFERPTSFGGTAVLQTLEPRLYYVYI
                     PFRDQSLIPVFDSGQQDINFATIYSENQFSGWDRINDANQVTLGVTSRLIGAATGAER
                     LRVGVAQRYYFDTQRVFLPGVAVRTSSSSDLLAAASGTVVPGWTAEAGWQYSTNFSQT
                     QRFNVGTRYQPQPGKVLNLSYRETLNVLRQTDVSTQWPVAQGWTALARWNYSLRERRT
                     LEGLLGAEYNGDCWVLRVVAHRFATTTQQTSTTFFIQLELNGVSRIGSNPMETLKRNI
                     GGYVRLDPRAPRPDESRSPYY"
     gene            38997..40019
                     /locus_tag="AC16CX_02515"
     CDS             38997..40019
                     /locus_tag="AC16CX_02515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR3011415.1"
                     /translation="MKANELQPSSDRRLEQLRGWLAARISEGPFTVEPASADASFRRY
                     FRVVAERHSWIAMDAPPEREDCRPFVRVAGLLRAAGVNAPEVLAQDLERGFLLLTDFG
                     DTTYLAALDETNAERLFSDAIAALIKWQLASREGELPPYDKTVLERECRLFPEWYVAR
                     HLGMTLSTAQQRTLSEMLALVISRALAQPAVYVHRDYMPRNLMVADPNPGVIDFQDAV
                     YGPISYDLVSLTRDAFVSWEEERVIDWAARYWDKAKRAGLPVDPDFSGFYRDFEWMGL
                     QRHLKVLGIFARIHYRDGKPGYLEDTPRFLNYACAVAGRYRELAPLARLFDQLEKREP
                     AVGYTF"
     gene            40038..40733
                     /gene="murU"
                     /locus_tag="AC16CX_02520"
     CDS             40038..40733
                     /gene="murU"
                     /locus_tag="AC16CX_02520"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HIR3011416.1"
                     /translation="MKAMILAAGRGERMRPLTEGVPKALLEAGGRPLIAHLIGRLARA
                     GFTELVVNVSHLAGLIERTLGDGSRYGVRIAYSREAQALETGGGIAYALPLLGEAPFV
                     VVNGDIYCDFDFTRLAAAVAALDPGTACAHLVLVDNPMHHPTGDFCLNGGKVSADGPP
                     PRLTFSGIGVYTPMLFAGVARGTRCQLVALLRPAMAQERVSGEHHRGLWMDVGTPQRL
                     ADLNRLLASAQET"
     gene            40778..42097
                     /locus_tag="AC16CX_02525"
     CDS             40778..42097
                     /locus_tag="AC16CX_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR3011417.1"
                     /translation="MTASIDTGIYRRRRERLAAAMRAGVAIVPTAPERNRNRDAHYPY
                     RFDSYFYYLTGFREPDAALVIVAGAEPKNILFCRDKDPEREIWDGFRYGPEAARAAFG
                     FDEAHSIDQLDALMPELLADRDALYCHLGADAAWDARVIGWINRVRARARSGVTAPSE
                     VKDVHALLDEMRLVKGPEELAVMRRAAAIAAGAHRRAMRMARPGRAEYEIEAELLHEF
                     RRHGAQAPAYSPVVASGSRACVLHYVQNDGMLCEGELLLIDAGCELDGYASDLTRTFP
                     VNGRFSGPQREIYDLVLAAQAAAIAAVKPGNRWDAPHRAAVETLSQGLIDLKLLSGGL
                     AEAVETEAYKKFYMHRTGHWLGLDVHDAGDYKRGGEWRALVPGMALTVEPGCYIRGGE
                     GVPQRFAGIGVRIEDDVVVTEAGAEVLTQDAPKRPADIEAWMRQRDA"
     gene            42090..43238
                     /locus_tag="AC16CX_02530"
     CDS             42090..43238
                     /locus_tag="AC16CX_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3011418.1"
                     /translation="MPDYDVLIVGGGPVGAAAALALRGSGLGVALLEARAPQAARSDP
                     RPIALSHGSRLILERLGMWERLAPVTPISRIHVSQRGGFGRVELDAREAGVPALGYVL
                     DYGRLAARLAGALEAEGGCHTVRATVSMLEAGPEAALARFDAGHGPEQATARLLAVAD
                     GGALTVARMRTRDYRQAVVTARVACEIPHQNVAYERFTPEGPLALLPSGERLALVWTT
                     TPEHAQQLCELPEAGFLARLQSVFGGRLGAFSRPSAPSRFPLALKVSNRIAKPRMVAL
                     GNAAQTLHPVAGQGLNLGLRDAWELAATIKDRNADEIGGAAHLKAYGALRRLDRGGGI
                     LFTDALVRLFSNDIVPLRLARGLGLAALGAVPPAKDFLIRRMIFGARG"
     gene            43615..44622
                     /gene="dusB"
                     /locus_tag="AC16CX_02535"
     CDS             43615..44622
                     /gene="dusB"
                     /locus_tag="AC16CX_02535"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR3011419.1"
                     /translation="MHIGPYQLKNNLIVAPMAGVTDRPFRQLCKAMGAGMAVSEMVAS
                     NSLLWGSEKTRRRANHDGEVEPVSVQLAGADPGMLAEAARYNVDQGAQIIDINMGCPA
                     KKVCNVMAGSALLKDEPLVGRILDTVVAAVDVPVTLKIRTGWDRENRNALKVARTAEQ
                     AGVKALTIHGRTRACGFSGEAEYDTIAAVKAEIGIPVVANGDLSTPEKVRQVLDYTGA
                     DAVMIGRAAQGRPWMFREIQHFLQTGERLPPPEVEEIHRVLLAHLNELYEFYGEDTGV
                     RVARKHISWYTKGLAGSCAFRHAVNRVETRAEQVAAVNEFFAQLAERGRRLTYVEELA
                     A"
     gene            44622..44858
                     /locus_tag="AC16CX_02540"
     CDS             44622..44858
                     /locus_tag="AC16CX_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3011420.1"
                     /translation="MNENEMARMVRRAIEGYFRDLDGEKPCAVYDMVIHCVEKPLLES
                     VLHRVRGNQTHAAEMLGINRNTLRKKMRVHGIKF"
     gene            44955..46517
                     /gene="purH"
                     /locus_tag="AC16CX_02545"
     CDS             44955..46517
                     /gene="purH"
                     /locus_tag="AC16CX_02545"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR3011421.1"
                     /translation="MAVIKQALVSVSDKTGIVEFARGLSGFGVALISTGGTARLLQDA
                     GLKVTEVADYTGIPEMLDGRVKTLHPKVHAGILARRDAPGHMAALGKMAIPTIDLVVV
                     NLYPFSAAVARAGCTLAEAIENIDIGGPAMVRSAAKNHEHVAVVTDPADYEPVLKEMA
                     ATAGALGAETRFRLAQKAFSHTAQYDGAISNYLTSLDAAGGRARFPQRLNLQFERVQA
                     MRYGENPHQEAAFYRDLQPAPGSLGAYRQLQGKELSYNNVADADAAWECVKSFEEPAC
                     VIVKHANPCGVAVADALAGAYRKAFATDPTSAFGGIIAFNRELDGPTAQAVTQQFVEV
                     VIAPAVAPEAAQRLAGKANIRVLEVPLAPGANDYDFKRVGGGLLVQMPDSASVGAAGL
                     KVVTRVKPTESQIADLLFAWRVAKFVKSNAIVFCGGGQTLGVGAGQMSRVDSARIAAI
                     KAQSAGLTLTGSVVASDAFFPFRDGVDAVIGAGARAIIQPGGSLRDEEVIAAADEHGV
                     AMVFTGYRHFRH"
     gene            46624..47901
                     /gene="purD"
                     /locus_tag="AC16CX_02550"
     CDS             46624..47901
                     /gene="purD"
                     /locus_tag="AC16CX_02550"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3011422.1"
                     /translation="MKLLVIGSGAREHALAWKLACSPRISRVFVAPGNAGTAREDGLA
                     NVAAVSIPELIAFARKQLVAFTVVGPEAPLAEGIVDAFQAAGLRIFGPTRAAARLESS
                     KEFAKRFMLRHGIPTAQYAAFSEPASAHEHIERSGAPIVIKADGLAAGKGVVVAMSAA
                     EAHAAVDRMLTEKRLGEAGARLVIEHYLDGEEASFIVLADGKHALPLATSQDHKRLLD
                     GDRGPNTGGMGAYSPAPVVSPAVHARIMREVILPALAGMEKDGTPYSGFLYAGLMITR
                     DGRVRVLEFNCRMGDPETQPILLRLKNDLLPLIEHALAGTLDKVEAEWDTRVALAVVL
                     AAHGYPEAPRKGDAIHGLPASQEDYHVFHAGTALHGNEVVTNGGRVLSVTALGGNLRA
                     AQRRAYEITERIRFEGMQFRRDIGHRALAAGRK"
     gene            47921..48187
                     /locus_tag="AC16CX_02555"
     CDS             47921..48187
                     /locus_tag="AC16CX_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011423.1"
                     /translation="MAMHYDRAREDPGNIVLLEHVDLTIPDQRLATIFYISGLGLTRD
                     PCLTIEHEVRSLTHPLYARPLVNRSPEQTNRDYARGHDSLRGAY"
     gene            48315..49292
                     /locus_tag="AC16CX_02560"
     CDS             48315..49292
                     /locus_tag="AC16CX_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011424.1"
                     /translation="MPAPFGIRRRFLAVVALLAVIPAAPAQQAFPGKKPIEFIVLFPA
                     GSSADVTARVLAEGMARQLETQIVVVNRPGGGGAVGYKYAAAQAADGHHLVWNSNSIS
                     TTYHTGTLPFDYRAFAPVARVQIETPLLFVRSEAPWKSLGEVLRYTKANPGRLTVGNS
                     GSGSHTHFSSVWLFRGAGVQVVDVPFNAAQVVPSLLGGHVDAVVQLPGALAGHVKAGT
                     VRALVALSGARDPHFPEVPTAREQGVNAAAELWRGVAVPRGTPPRIVARIEDAIRKTV
                     SGPEFARATEKLLVAPAFLPASDFGKLIAREDTEIAQAVKALGLKPDSK"
     gene            49297..50196
                     /gene="hemF"
                     /locus_tag="AC16CX_02565"
     CDS             49297..50196
                     /gene="hemF"
                     /locus_tag="AC16CX_02565"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HIR3011425.1"
                     /translation="MDIAAVKQFLIGLQQAIVARLAEADGQGFRRDEWTRPEDGGGIT
                     QLIEEGALFERAGVNFSHVSGQGLPPSASASRPELAGRSFQAMGVSLVLHPRNPYVPT
                     VHMNVRFFVAQKNGAGPVWWFGGGMDLTPYYPFAEDARHFHRTCRDALAPFGPDRYPR
                     YKRWCDNYFYLKHRAEPRGLGGIFFDDLNEPDFATCFALVKSVGTHFLPAYVPILERR
                     RGIAYGSRERAFQAYRRGRYVEFNLVYDRGTLFGLQSGGRVESLLVSLPPRVEWRYDW
                     KPEPGSPEEKLYTEFLVPRDWAD"
     gene            complement(50308..52239)
                     /locus_tag="AC16CX_02570"
     CDS             complement(50308..52239)
                     /locus_tag="AC16CX_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3011426.1"
                     /translation="MNTSSTIGKYQIQDVLGQGGMGIVYRAFDPAIHRPVAVKTIIKS
                     ELDPADLQYALTRFRHEAQAVGRLTHPRIAAIYDYGEDTEMAYIVMELVNGHSLFHHI
                     QNQAKFGLKEIGELVRQLLDGLGYAHSLGVVHRDIKPSNILINDDGRLKLTDFGIARL
                     DSSTLTQVGEIMGSPGYMSPEQFLGTEIDARSDIYSVGVIAYELLAGRRPFTGNNAEV
                     MRQALNERPVNPSEYNPKISVQLDWAVQKALAKKREDRFQTAKEFADAFVKGIEASIR
                     QSQRETAAAAIADEPTQRIDPKLVQAARMLADLKQATVDMPTQPSMPTPAAAPAAVPA
                     AVPAGLATETSIKKARLLFVDDEERILTALRSVFRMNYHVFTATNGPEALEFVRKFQP
                     HVVVSDQRMPEMTGVELLRRVKEAAPATVRLLLTGYSDLAAIVGSVNEGEVFRFVSKP
                     WDNQDIQNTIGEAAAIALELAETAATPPIVPDKMEAGVLVIEAREATLRAVKRFVGGA
                     CPVRHAKDFDAALGQLEAHETALIIADIASRGEESLAAFKVLKHERPEILVIVLTEAS
                     DSELVIELINQAQIFRFINKPVNPELLKSHTQAALIRYQSFKQNPHLTHQHKVAAAGV
                     EGSVSRKILARVKSLKSWF"
     gene            complement(52265..54124)
                     /locus_tag="AC16CX_02575"
     CDS             complement(52265..54124)
                     /locus_tag="AC16CX_02575"
                     /inference="COORDINATES: protein motif:HMM:NF039312.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3011427.1"
                     /translation="MTEPTTLLAGLQRILAVKTARRVLIAAGAILLAAILGYLYVKTQ
                     GADIKRSNEVLAALRELKEIDARWDVDILRLRTEFAPPQTPSPDYGPALARLGKELGA
                     AAQALGSPVLARGLPDLASAFSQKAGMVDKFRKANAATKQMLARVLAAEAEIAGLVRG
                     SWQDFRERERLVAAESAAVQAVAETQRYYFTPSEAQRKLVETVISDLRQAAGHLPPAL
                     RDGIHRLDDHVQQLLGARPVEEALFVKLSFLTAGPRVDSLTNAFSRELEMVLADQEFY
                     RAYLVAYSGALLILLGYLGTRLIASYRLLNAANAELKSVNEGLELRVAERTQELTEAL
                     RQLKESEAQLIQTEKMSSLGQMVAGVAHEINTPLAYVKNSLGSVTGKLPELVRLAAES
                     EKLLELLRSGAADPHQLAQQFALTEQLIGQFREHRVLDELQTLVKDGLHGINQISELV
                     LNLKNFSRLDRSKVTSFNLNEGLESTLLLAKHELKHHIVKKNFGDIPAVTCSPSQINQ
                     VFLNLINNAAQATESGKGVIQLTTRREGAGHVAVEVQDNGKGIPPEVLPKIFDPFFTT
                     KDVGKGTGLGLSIAYKIVQQHGGSISVDSAVGVGTKFTVVLPLTPPEPAQQAA"
     gene            complement(54132..54836)
                     /locus_tag="AC16CX_02580"
     CDS             complement(54132..54836)
                     /locus_tag="AC16CX_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011428.1"
                     /translation="MLEVRGLKVSYGGINAVKGIDLEVKAGELVTLIGANGAGKTTTL
                     KALAGLLRPAAGRIHYNGDDITARPAFELVRRGLALVPEGRGVFGLLTVEENLAMGAY
                     SRRDSARITADFDRVYGLLPRLAERRRQLAGTLSGGEQQMLAIGRALMSRPRLLLLDE
                     PSMGLAPLMVQKIFETIRAIAAEGATLLLVEQNAKLALEVCHRGYVMESGLIAAADSA
                     RRLLVNPQVRQAYLGE"
     gene            complement(54829..55614)
                     /locus_tag="AC16CX_02585"
     CDS             complement(54829..55614)
                     /locus_tag="AC16CX_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011429.1"
                     /translation="MTRHAESGALLSASAISKRFGGLAALAEVSLTVERGEIFGLIGP
                     NGAGKTTLFNVLTGLYRPDSGEFAFDGVSLTGTKPHRVVECGIARTFQNIRLFANMSA
                     LENVMVGRHARTRAGVFGAVLRDAATRAEEAAVAGRAYALLDYVGIAGRAAEPAYSLS
                     YGDQRRLEIARALATEPKLLALDEPAAGMNATEREELKRLLARIRGDGMTLLLIEHDV
                     KLVMGLCDRVAVLDYGRKIAEGAPQEVRRDPAVIAAYLGGELA"
     gene            complement(55598..56728)
                     /locus_tag="AC16CX_02590"
     CDS             complement(55598..56728)
                     /locus_tag="AC16CX_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3011430.1"
                     /translation="MLELGQRWQRWRRDPRFVWVAYLLLAAALIALPFVTDWAVGRTW
                     VRIAAFALLYIMLALGLNIVVGYAGLLDLGYVAFFAVGAYLYALLASPHFNLHLPFWV
                     LLPGCAVFACVFGVLLGAPTLRLRGDYLAIVTLGFGEIIRIFLVNLNAPVNITNGPQG
                     VNLIDPIRLGEWSAAQTHSFFGLTFPTVYNYYYLFLALTLAVVFVSMRLEDSRIGRAW
                     VAIREDEVAATATGINTRNVKLLAFAMGASFGGLSGGLFAGFQGFVSPESFTLIESVM
                     ILCMVVLGGMGHIPGVILGVVLLVVLPEALRYLGPLQQALFGRVLADPADLRLLLFGL
                     ALILVMLLRPSGLWPSPVRRRELAAEGAIARQEQASVYDAAR"
     gene            complement(56824..57753)
                     /locus_tag="AC16CX_02595"
     CDS             complement(56824..57753)
                     /locus_tag="AC16CX_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3011431.1"
                     /translation="MDIFLQQLINGIVLGAIYALVALGYTMVYGILGLINFAHGEVVM
                     IGAMVALTVIQLLMASGVALPGPLIAALGLAAAIPACMALGYAIERVAYRPLRQAPRL
                     AALITAIGMSILLQNVAMIIWGRQYHSFPALLPSTPHALAGATVTDLQVFIVALAFAI
                     MAGLLWLVHRTRLGRAMRATSQNPEVAGLMGVNINTVISITFVIGSALAAVAGLMVGA
                     YYGIAHYSMGFMLGLKAFTAAVLGGIGNLAGAMLGGVALGVIESLGAGYIGALTGGFL
                     GSHYQDVFAFFVLILVLVFRPSGLLGERVAERS"
     gene            complement(58039..59253)
                     /locus_tag="AC16CX_02600"
     CDS             complement(58039..59253)
                     /locus_tag="AC16CX_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3011432.1"
                     /translation="MVRQSVFGIGWGLAFALILSSCGQEAPPPKAGAPKAPPEIVIKI
                     GQVSPLTGPQAHLGKDNDNGARLAIEELNAKGLMIGGVKVKFELVSEDDQADPKTGTI
                     VAQKLVDAKVAGVIGHLNSGTTIPASKIYHDAGIVQVSPSATNPKYTQQGYKNAFRVM
                     ANDIQQGKVLGEYAVAKLGAKRIAIIDDRTAYGQGLADEFEKAAKAAGATVVGREYTS
                     DKATEFRSILTNVKARQPDLVFYGGMDAQAGPMVAQMKALAVKARFLAGDGAQSPEFI
                     KLAGAAAEGVVASSPGLPLDKMPGGTGFTERFNAKYGQIQIYAPYAYDATMTLVEAMV
                     KANSTEPAKYLPELAKIQRQGVTGPIAFDEKGDLRNGPITLYVVKESKWDPLETVSGG
                     AAAAPAPAAEKK"
     gene            complement(59398..59724)
                     /locus_tag="AC16CX_02605"
     CDS             complement(59398..59724)
                     /locus_tag="AC16CX_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011433.1"
                     /translation="MKNILTLLAAAAAATIAAGAHAADPKTAEALAKTSGCLACHTVD
                     KKLIGPSYKDVAAKYRGNKGAEAELIKKVKAGGKGVWGDIPMSPNAHVKDEDIKTMVQ
                     WILSLK"
     gene            complement(59847..61889)
                     /locus_tag="AC16CX_02610"
     CDS             complement(59847..61889)
                     /locus_tag="AC16CX_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3011434.1"
                     /translation="MPNRLATQTSPYLRQHADNPVDWYPWGEEALAAAREAGKPILLS
                     IGYSACHWCHVMAHESFEDPEVAAEMNRHFVNIKVDREERPDLDQIYQTAHAMLTQRS
                     GGWPLTMFLMPDGTPFFGGTYFPKRARHGMAGFLDILPRIADAYRDKREDIERQNAAL
                     LEALSRTLPAPGSGRSLKRAPLDAVVRELAQVFDDLHGGIGQAPKFPHPFELAFCMRR
                     HALDGGELGLSIARLTLAKMAEGGIYDQLGGGFCRYSVDEYWSIPHFEKMLYDNAALV
                     ALYSDAWLVTRQPLFEKTVRGIAQWVMREMQSPEGGYYSSLDADSEHEEGKYYVWTPE
                     ELKSLLTAEEYAVIEPHYGLDGAPNFEGHWHLRMMKPLEFIAKRLSIPLAECEARLAR
                     ARMKLLAAREQRVRPGRDEKVLTSWNAMMVGGLARAARAFGEGAWLGSARRALEFIRA
                     TLWRDGRLTATYKDGHAHLNAYLDDHAFLLDALLELMQGEFREDDLRFARELAEILLE
                     RFEDRETGGFFFVSHDHEKLIHRAKPGHDNATPSGNGIAALALQRLGHLIGEPRYLGA
                     AERALKLFYPAMERQPSGFVSFATVLDEYLAPPQIAILRGEARALAEWQHALARTYRP
                     DTLVLALPAGLAGLPPVLDKPAPPAGVNAWVCRGVTCLPAVTDLAGLKRILSTGHG"
     gene            complement(62040..62942)
                     /locus_tag="AC16CX_02615"
     CDS             complement(62040..62942)
                     /locus_tag="AC16CX_02615"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HIR3011435.1"
                     /translation="MTAWLLAGCATPQVAALLARPPSDLPGRVELDAVPFYPQELYQC
                     GPAALATTLVHAGVATSPEALVPQVYLPGREGSLQAEMLAAARRHGLVAYRLAPRLEH
                     VLQEVAAGTPVVVFQNLSFAFAPLWHYAVVVGYDLPREEIMLRSGTTRRLVTTLSNFE
                     RTWARGEHWAMVTMPPDRLPVSAAEGSYVAASAALERVSPAAAGRAYASALERWPHNL
                     FARIGSGNTAYAMRDLPAAEAAYREATRRHPEAADAWNNLAQTLLELERRDEALAAAQ
                     RAVAIGGPRLAQYEATLKAITDAR"
     gene            complement(62993..63373)
                     /locus_tag="AC16CX_02620"
     CDS             complement(62993..63373)
                     /locus_tag="AC16CX_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR3011436.1"
                     /translation="MKILNRCVARVLIVCTLGMGMPLPASAGIVTTDQIVAGAERDQV
                     KNFLDRADVRAQMQALGVDAGATRARVDALTDEEARDLAARIDQLPAGGSDVLGVLLV
                     IFIILLVTDLLGLTKVFPFTRSSR"
     gene            complement(63490..63669)
                     /locus_tag="AC16CX_02625"
     CDS             complement(63490..63669)
                     /locus_tag="AC16CX_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011437.1"
                     /translation="MKAFAQRVVEARWFEPAMIGPFVLNGALIGLETSHDSLPGSRPS
                     RRKSLNLMNLRNNSR"
     gene            complement(63686..65359)
                     /gene="ilvD"
                     /locus_tag="AC16CX_02630"
     CDS             complement(63686..65359)
                     /gene="ilvD"
                     /locus_tag="AC16CX_02630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR3011438.1"
                     /translation="MPLNKRSQLITQGVQRSPNRAMLRAVGFKDADFRKPIIGIANGY
                     STITPCNLGLDDLTRRAEAAAKKAGAMPQTFGTITVSDGISMGTEGMKYSLVSREVIA
                     DSIETAVQAQRMDGVLAIGGCDKNMPGALIAIGRMNIPSIFVYGGTIKPGHYQGRDLT
                     VVSSFEAVGAYTAGKIGKEELTGVERRACPGAGSCGGMYTANTMSSAIEALGMSLPGS
                     STMAAEDKEKHDSAAESAEVLIRAIRKELLPRAIMTRKSFENAISVVMAVGGSTNAVL
                     HLLAMAHAAEVPLTIDDFETIRHRVPVLCELKPSGRYVITNLHEVGGIPQVMKMLLAH
                     GLLHDDCLTISGKTVGDLLRKVPSRPPLNQDVIRQWENPLYPHGHLAILKGNLAPEGA
                     VAKITGLKKKSITGPARIFESEEDCLTAILGGKVKAGDVLVIRNEGPKGGPGMREMLA
                     PTSAIIGAGLGESVGLITDGRFSGGTWGIVVGHVAPETAVGGPIALVHEGDSITIDAE
                     RLLLQINVSEEELARRRAAWRSPRPRYTRGVLAKYAAQVSSASRGAVTD"
     gene            65432..66217
                     /gene="lgt"
                     /locus_tag="AC16CX_02635"
     CDS             65432..66217
                     /gene="lgt"
                     /locus_tag="AC16CX_02635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3011439.1"
                     /translation="MLVHPQFDPVALHIGPLAVRWYGLMYLVGFALLWAAGRYRIARS
                     SGSVWTARDLDDALFYGILGTILGGRLGYVLFYKFSDYLAEPWRILYVWEGGMSFHGG
                     MLGVILAMAWFARSRRQDWLAVTDFLAPLVPLGLAAGRLGNFINAELWGRPASVPWAM
                     VFPNVDGVPRHPSQLYEFALEGVVLFAALWWFSARRRPRGAVSGAFLLGYGLLRFLVE
                     FTREPDRFLGYLALGFSMGQWLSAPMILAGIALIAWAYRPAAR"
     gene            complement(66282..67055)
                     /locus_tag="AC16CX_02640"
     CDS             complement(66282..67055)
                     /locus_tag="AC16CX_02640"
                     /inference="COORDINATES: protein motif:HMM:NF018928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HIR3011440.1"
                     /translation="MEIKPPYGYQEIVPLTRQHRVLLPEAGKLATVFHSLAALPISFT
                     EFAVACRDYPIAFIGGEGGHFVAMAVLGLENQQNLFVTPNGGWDPATYLPAYVRRYPF
                     CMTRVTVDGRERPERVACVEKRAINNKGEALHDAKGEPLPTWEERRKLLFEYEADLVR
                     CEEMSSELARLGLLETFIMQAIPNQGTPLAMTGMYRVAEGRLTELAPEKLKELTQRGI
                     LARVYAHLISLANFGRLLDRRAALAKPASATAGGLKKPN"
     gene            67172..67247
                     /locus_tag="AC16CX_02645"
     tRNA            67172..67247
                     /locus_tag="AC16CX_02645"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67205..67207,aa:Ala,seq:cgc)
     gene            67380..>67912
                     /locus_tag="AC16CX_02650"
     CDS             67380..>67912
                     /locus_tag="AC16CX_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR3011441.1"
                     /translation="MFGKLLPREGRFFEFFNEHAELIVQGSRELAALMEGGSDLALRA
                     HNIETIEKRADRITRSTIELLHKTFITPLDRDDIHKLITKMDDILDLIEDAAQLVVIY
                     DIRVVTAEEKRLADICVTCADGVKSAVGMLSNMENAAAMLAACEDIDRLESEADHVMR
                     AAMAKLFRDEPDVRELI"
BASE COUNT        11437 a        22037 c        22307 g        12131 t
ORIGIN      
        1 tcgatcttct gatcgagggc atcaagcggc tcgaataccg cggctacgac tcgaccggca
       61 tcgcctacgt caagggcagc cggcttgagc ggctgcgctc caccggacgc gttgctcatc
      121 tggacgagct tgcgcgcggt cagggcgtgc tcgccagcac cgggctgtcg cacacgcggt
      181 gggcgacgca cggcgcgccc acggaggcca acgcacaccc gcacttttcc gcccgcggcg
      241 gcctggaaat aggcgtcgtc cacaacggca tcgtcgagaa ccacgagccg atccgcgccc
      301 gcatgaagac gctgggctac ggcttttcct cggaaaccga caccgaggtg atggcgcacc
      361 acgtgcattc cctggtggcc ggagggctgt cgctgttcga ggcggtcaag gccgcgagcc
      421 gggagtggca gggtgcgtac gccgtcggct tcatatccag ccgggagccg gacgtcgtgg
      481 tgggcgcccg ccggggctcg ccgctgctcg tcggtcttgg gcagggtgaa aactttctcg
      541 cctccgacgc gagcgcgctc gtggcgcgga cgcgcgaggt gatctacctc gaggagggcg
      601 acgtcgtcga ggtgcgcccg caaggcgtca cgatctgcga cggtgacggg cgccccgcgc
      661 aacgcgcggt cacccgcagc gagctttccg cggacgcggt cgagaaaggc ccgtacgcgc
      721 attacatgca gaaggagatt ttcgagcaac ccggtgcggt cgccaatacc ctggaaatgg
      781 tggcgaacgc gcagtccctg gtacccaatc tgttcggcgc cgaagcgcgg gaggtcttca
      841 gccgcacgaa ccagctcctg ctgctcgcct gcggcaccag ctaccacgcg ggcttggtgg
      901 cgcgctactg gctcgagtcg gtcgcgggcc tgcccgcgac ggtcgagatc gcgagcgagt
      961 tccgctaccg cgatcccgtg gcgcaacccg ggacgctggt cgtgacgatc tcgcaatcgg
     1021 gcgagaccgc ggacacgatc gccgcgctca agcacgcgca ggcgcgcggg ctggccgata
     1081 cgctgacggt gtgcaacgtc cccgaatcag ccctgatgcg ggcttccagg ctgcgcttcc
     1141 tcacccgcgc cgggcccgag atcggggtgg cttcgaccaa ggcctttacg acacagctcg
     1201 ccgccctctt cgtcctcacg ctggtgctcg ccaagctcaa gcagcggctc gcgcccgagc
     1261 gcgaacagga gttgatcgcc cagctgcgcc acctcccgcg agccatggaa gcggtgctct
     1321 cggtggagcc gcaggtgcgc gactgggcgc ggcgcttcca gcccaagcac catgcgctgt
     1381 tcctggggcg tggcgtgcat ttcccgatcg cgatggaagg ggcgctcaag ctcaaggaga
     1441 tctcgtacgt ccatgccgag gcctacgccg ccggggagct caagcacggc ccgctcgcgc
     1501 tggtggacgc cgacatgccg gtcgtcacgg ttgcccccaa cgaccagctg ctggaaaagc
     1561 tcaagtcgaa catgcaggaa gtacgcgccc gcggcggcga actctatgtt ttcgccgatc
     1621 gcgatgcgcg cgtcgccgca gccgagggcg tgcacgtcat ccggctcgtg gatcatgcgg
     1681 gagcgctctc gcccatcctg cacgtgatcc cgttgcagct cctcgcctac cacgtcgcgg
     1741 tgctgcgcgg caccgatgtg gacaagccgc gtaacctcgc caagtccgtc actgtggagt
     1801 agtcagtgac agtccgaccg ttacccgacc agagtgcggc ttgcggcgca ggctaactgc
     1861 gcgtagcgca tgttaagcgc gcggcttcct tcgcttgctt cagcaagcac ctagccgcgc
     1921 gcggccggag ccacaagccg cggaatttgg cgaagtcagt cactgtcgag tagctctatc
     1981 ccgcccagcg ccgtggcttg cgccgcgcgc cggcgacgag ccggtcatat gcccagttcg
     2041 gcagcacgtg cagaatgcgt gcgatgagcg ccatctgcca cggaatcact gaatatgcct
     2101 tgccgcgcgc gatgagggac gcgatcttcg cggccgcctc gtctgcgccc atcaggaacg
     2161 gcatgggata cggattcttc tccgtcatcg gtgtcgcgat gtagccgggg cagatggtga
     2221 tgaccttgac gccgcttcca cgcaactcca cacgcaaact ttcgaggtag gcgatcgcag
     2281 ccgctttcga ggctgagtat gccgccgcgc cgggcagccc gcggtagccg gcgacgctgg
     2341 cgatgccggc cagcgcgcca ccgtcccgct cccgcatgcc ggccacgaac ggccggaagg
     2401 tgttgaccat gccgatcacg ttggtgtcga tgacctcctc gaacgcacgc gcgtcctcgg
     2461 caaattcggt gagcgtgcca acgctcacgc cggcgtttgc gatgacgaca tcagggcaac
     2521 cgtgccgctt gataaagtgg cgcgccgcgg cggcaagcgc cgcagcgtcg cgaacgtcga
     2581 gagggtaaat ctcgcagcgg ctcgcgaaac ctgcagcgag ccgctccagc tcgggcttgc
     2641 gccgcgcgat cagtcccaag gttgcgcctg aagctgagta gcggcgggca agggccgcgc
     2701 cgatcccgct ggaagcgcca gtgatgatga tgcgttgcgc tgatcgcatg gaagacgctt
     2761 atgccggagg acagcgagtt ctcgctttat cgccgcccat cggcggctca gcggcttccc
     2821 atgagccatg ggacggcgtt ctggcagccc gggtggcggc gagcgatatg gccgccgata
     2881 ctacttttcg ctgcgcttct gccgcgcgag gcggatgagg tcctcgagca ccggaaccac
     2941 gccgcgcacg gcgggcgtca tgctgctcga ggtgaggtag cgcccgtcaa ccaccacgct
     3001 gggcgtgccc tggacgtcgt agcgctgggt gagttgttgc gcgcgcgcga ccttcgcgtc
     3061 cacctcgggg gaggagtagg cgtcgagcca cttcctgcgg tcaatgccgt tcttgaccgc
     3121 ccattgctca atcacgtcgg gcttgctcat gtgcagctcc tcgacgtggt agctgtaata
     3181 gaccttctgg tgcaggcgct cgacttcgcc cagcagctcg agcgtgtaat agatgcgggc
     3241 gtgcggcgcc caggtgtcct tcagaatgac cgggatccgg cgcagcgcca cgtcggcggg
     3301 cttgcgcttg agccactcct ccagcgccgg ctgcaactcg ttgcagtacg ggcagccgta
     3361 ccagaagaag tcgatcacct caatccggtc gccggtttcg accggctgcg aggcgatcag
     3421 acggtattcg atgttctgcc gcgcgcgaat ttcctgcgcc ggcgccgtcc cggccagacc
     3481 gatgagggcg agcagtgtca gcccgccgcg cgcgagaagg aagggcaggt ttcctcccat
     3541 ggtgcggacg ctcctatttt ttcccggcgc gctgcttacg cgccatggcg atcacctgat
     3601 cgagcacctg gaagtagcgc tcgtaattca cgttttcgcc gtccctgaga cccagcgatg
     3661 gcgcggtgat aaaacggcca tttacgacca gtgtcggcgt gaatcggatg tcgtaactct
     3721 cggtaacctg catcgtgcgc tgcgtgcggg tgttgaccgc aaaggaattg taggtatcga
     3781 tgaatttctg ccggtccgcg cccttgctcg cgatccagtc gaataacggg ctgggattgc
     3841 tcaacaaggc cttcggattg agcgcccgtt gctcatggat cgcggcgaac agatcacggt
     3901 gcagccgctc gaccagcccc atcgcttcca gtgcgtagta agtgcgggcg agctgcagcc
     3961 acgactcctg gaatgccgcg gggacccgcc tgaactcgac gtctgcaggc ttgcgcttga
     4021 gccatgcgtg caaggacggc tgcaagctgt tgcagtgggg gcaagcgtac cagaaaaact
     4081 ccaccacttc gactttcttg ccgctgttgg tcggctgcgg cgggttaatg agaatgtagt
     4141 ccttgcctgc gacgagctgc gcggaagcgt tggcgctgac ggtgagcgcc agcagccata
     4201 ccgcaagccg taagcaggat gggataaaac tcattttgtc ttcgggaaaa agtaagggtc
     4261 cggcgctatt cgcgcacctt gatcagggcg gtctcgacgc cgttctgctt cagagtatcg
     4321 cgcgtgcggt tgagttcctc gacgctggta tacggcccga cgcgcacccg gtgccacacg
     4381 cccctgtccg gcagggtggt cgtctggatc ttcgcctcga tcccgagcag cgcgagccgc
     4441 gccttgaggt tgtccgcatc gggggcgctc tggaacgcgc ccgcctgcag gaaaaacgtc
     4501 tccttggccg ccgcagtcga cttcttctgc gattccctca attgctggtc ggtggccggc
     4561 tcttcggttc cgggaaggat cttgtagaag tcgaagcgcg gcttggcttc gtgcgccctc
     4621 gccgtctttt cctcgctcct tgccggcgcc tgcgcgccct tggcgggctc gcccttggcg
     4681 ggaggcgtgc ggctcaagaa cgggctcggc atcttattga tgtaccacgc caccgcgagc
     4741 gcgatgccga ggcccagcac gagaccgatc agaatgccga tgatgagcga gctgccgccg
     4801 ccgcgggatt tcggctccgg tcgcttgtaa tccttggaca tgcggatatc ttacatttta
     4861 tccggcgcgg agacacccag cagcgcgagc ccgttgcgca gcacctgccg cacggcggcg
     4921 gcgagcacga gccgggcgag cctgatgtcc tcctcttcca ccagtatccg ggtcgcgttg
     4981 tagtagctgt ggaattcccc cgcgagttcc cgcaggtaaa acgcgatcag gtgcggcgac
     5041 agatcgtgcg ccgcgatttc caccgcttcg gggtattcca tcaagcgcgc cagcagcgcg
     5101 agttcgtgct ccgtgacgag agcggacaca tcagcggcga caagcttccg cacgcccccg
     5161 ccccactgct cgagcacgct gcacacgcgg gcatgcgcgt actggacgta gtagacgggg
     5221 ttttcgttgc tctgcgactt ggcgagatcg aggtcgaaat cgagatgctg gtcgcttttg
     5281 cgcagcacgt agaaaaaacg cgctgcgtcg ttgccgacct cgcgcctcaa ctcgcgcagc
     5341 gtgacgaatt cgccggagcg cgtggacatg gagaccctct ggccgttgcg gtagagcacg
     5401 gcgaattgca ccagcgcgat ggtgagctgc tcgggattca ggccgagcgc cgccagcgcg
     5461 cccttgaccc gcggcatgta gccgtgatgg tcggcgcccc acacatcgat caccgtctcg
     5521 aagccgcgct cgaacttgtt caggtggtag gcgatatccg aggcgaaata ggtgtactgg
     5581 ccgttctcgc gccggaccac gcggtccttc tcgtcgccaa aggcgctgga acggaaccag
     5641 ctcgcgccct cccggcggta gacgtggccc cgctgctcca gcgcgctgat cgcgcgctcg
     5701 accaggccgg agtcgtagag cgagcgttcg gaaaaccagc gatcgaaagt aaccccgaat
     5761 tcggccagat cggaacggca gtcctcgagc tgctcgttga gcgcgaaatc gtggatgtag
     5821 gcgtaatccg cgcccagctt ttccttcgcc agcccgatca acgcatccag ccgggtttcc
     5881 tcgtcgggct cggccgtcaa cgtcgtgaag acatccactg cgatgccgcg cctgaactca
     5941 tcgccgtgct gggcgcggat gttgagcgcc atgcgccgca cgtattcccc ctgataggcg
     6001 ttggggggaa agtccactgt gacgccgcac gcctcgagat aacgcaacca ggtcgagagc
     6061 gccaggatgt ccatctgccg cccggcgtcg ttgacgtaga actcgcgggt gacgtcatat
     6121 cccgcagcgg cgagcacgtt ggccaggctg gctccataag ccgcgccgcg cccatggccg
     6181 acatgaagcg gcccggtggg attggccgac acgaactcga cctgcacccg cttcttctgg
     6241 gcgggcgcga tggtgccgta agccgtgccc gcttggagaa tgcggttgac cgcttgctgc
     6301 ttgaactccc gcgtcagaaa caggttgatg aaaccggcgc cggcgacttc ggctttttcg
     6361 agatggggcg aggccggcag cgcggccacc agctgcgagg cgatttcgcg cggcgcacgc
     6421 ctcagcgtcc cggccaattg cagcgcgacg ttgcaggcgt agtcgccgtg cccggcgcgc
     6481 ttggggcgtt cgagcacgat ggctgcgtcg gtgcggtccg gcgccacctt gcgcaaggcc
     6541 tccgcaagca ggccggcaag gagcgcacgc gcgtcgtcaa tgggcgccaa attcattccg
     6601 cgtacgtgca ggaaagtgga atcgactgcg ggcgcggcgg ccggctggcg tgcggacggc
     6661 cgtacttgcc tgggctcggg cgcgccctgt tcgccgcatt cgcgatctga tggagcacgt
     6721 gctcgctgac ggggggatgc atactaggcg atccctatgt tatgccattc tgaccgatca
     6781 gaaatcgatc ggatcgacat cgagcgccca acgtgcccgg gtgacgcgca cgcgggcgag
     6841 cacgccgtgc caggcgccaa gaaaatcccg caatcgctcg cgcgaggcgg actgcaccag
     6901 cagttgcgcg cgttcgcgtc cggcacgccg cggcatggca gccgggaccg ggtcataagc
     6961 agtcacgcgc gcatcgagcc ccttgcccac gcgcgcggct tcgttgagga actcgagcgc
     7021 ggtggcgagc cgtggcgctt cggcccgtaa catcgcctgg tagacgaaag ggggaaatcc
     7081 cgcctgtctg cgctcggcga gcgcgctgtc cgcgaatgca cgatagtcct gctggcgcag
     7141 tgccgcgtac agcgggtggt ctggaaactc ggtctggatg agcacctccc cccgcgcccc
     7201 gcctcggcca gagcggcctg caacctgggt gagcaacgcg tacagctgct cggcggcacg
     7261 gaaatcgttg ctatagagcc ggctatcggc attgagcacg cccacgaggt tcaaatgagg
     7321 gaagtcatgc cccttggcaa ggatctgggt gccgaccagg atatccactt ccctgtcctg
     7381 gatgcgctgg cgcatcgtct gccaggcatg acgccggctc gtggtgtcgc ggtcgatgcg
     7441 cagggtgcgc gcttggggaa aatgccgcgc cagcgcctgc tccacgcgtt gcgtaccctg
     7501 tccgatcggt gccagatccg gattgccgca gccggggcag gcggagggcg ccggcgagac
     7561 gtggccgcag tgatggcagc gcaggcggcg ctcggtgaag tgcagcacga gttgcgccga
     7621 gcaccgtccg cagcccgaga tccagccgca cgcgcggcac atcaacaccg gggcatagcc
     7681 ccgccggttg atgaatacca gtgactgctc gccgccggca agccgctcgc gcagcgccgc
     7741 cagcagcggt ctcgacagcc cgtcgacaag tttctcgccg cgggtgtcga tgcaggtgat
     7801 gcggggaggc ggggcgccga tgcggctcga cagcgtgagc agcgaataac ggccggtgac
     7861 cgcattgtgg taggtctcga gcgcgggagt cgcagaggcc agcacgacgg gaacgccatg
     7921 ctggcgtgcc cttgccacgg ccagatcgcg cgccgagtaa cggaagcctt cggcctgttt
     7981 gaaggaaccg tcctgttcct cgtcgaccac gatcaggccc agctccggca gcggcgcaaa
     8041 caccgcgagc cgggtgccga gcacgacgcg agcccgcccc tcgcgggccg cccgccagtt
     8101 ttccaggcgc tccgagtcgg caagcccgct gtgaaggctc gccgtgcgcg tgcccggaaa
     8161 gcggtgtccc accaaagctt ccagctgcgg cgtcaaggcg atctcgggca ccagcagcaa
     8221 tgcctgtctt ccggtcttga gcgccgcggc gatggcgtgc aaataaacct cggtctttcc
     8281 gctgccggtg acgccaagca ggaggaatgg ccggtacttg cccaggccgg cgcagatttc
     8341 atttaccgcc ctcgcctgtt cagcggtgag ctcacgggaa gccacggtga acggtaatgg
     8401 gggcacgtcc gttttcctgt cctcttcgag agttatccag ccgcgggaaa ggaactgttt
     8461 caggagggat cgcgcgttgg gaacgagggt tcttgcctcg gcatgcccca gcggcccttg
     8521 atgcaggcgc tccagcacac ggcgctgggc gcgagcgcgc agcggcagcg cggatgcgtc
     8581 gagcgcggcc ccggcggcag tgaggagata agcgccgcgc tccctcggct gtgaccgacc
     8641 ggcaacccgg cgcagccgcg tcggcagggt gcccatcacc accgcgccta agggatgatg
     8701 gtagtagtcg gcggcgaacc gcatcaggcg cagatcttcc gccgccaagg gcgatagatc
     8761 acgcaagacg cgcagtgcgt ttttcaggcg ctcgaccggt accccgctcg cgctcgaaac
     8821 ctcgacgatt acgccaatcg cggttctgtt gccgaacggc accacgacgc ggcggccgat
     8881 gtcggcagcg gttgcgtcgg gcgcccgata atcgaataac gtatcgaccg gcacatcgag
     8941 cgcaacgcgg ataattggca tgccgatgtt ttgtcgtgtc gtgaacggtg tgggaaattg
     9001 cgccagcgag cagaaaacgt cccggcgtgg cggcgccccg cccctactag atgcgcaacc
     9061 ccgaggttat caacagagtg ctgtggacaa ccttgttgat agtgcattcg atttggcgat
     9121 cgaccctaac cgttagcgga gaggcaacgc atccgtcatg caaggaagcc aataaaatcc
     9181 catataaaac aacaggataa gttaaaagtc gagatctgta acaatccaat gggaaagccc
     9241 ctctgaaata acagattact gtgcataagt cagagatcct gcccgcaaag cctggacagg
     9301 cgcggatctt cagtgatagg ccgggctcaa ctcgcgcacg gcctctacca gcgcctgtac
     9361 gcaatcgggg gaggtgaatt gagaaatgcc atggccgaga ttgaacacgt ggccggcgcc
     9421 aggaccaaat gccgcgagta tggccgccgc gccgcggcgc gccgcctcgg gaccggcgag
     9481 cagagccgcg gggtcgaggt ttccctgcag cgcaacgcgt tggccgacga ggtcacgcgc
     9541 cgcgccaatg tccaccgtcc agtccaggcc gaccgcgtcg cagccgctcg cggcgagcgc
     9601 ttcgagccag gctgcgccgc ccttggtgaa caggatggtc gggatgcgct cgccttcgtg
     9661 ctcgcgcttc accccatcga gcacgcgctg catataggcg agcgagaatt cacggtattc
     9721 ggcggcggcg agaacgccgc cccaggtgtc gaaaatcatc accgcttgcg cgcccgattc
     9781 gatctgtgca ttgaggtagg cagtgaccgc acggcggttg acctccagaa tgcagcgcaa
     9841 caagtcagga cgggtgtaga gcatggtctt cacggtgcgg aaatcggcgc tcgcgccccc
     9901 ttccaccatg taacaggcga gggtgaaggg actgccggag aagccgatca acgggacctc
     9961 ggcggcaagc gcccggcgca cctgacgcac cgtgtcgagc acgtagcgca gctgctcgcc
    10021 gggctcgggc gcgacgagac ggcgtatttc ccgctcctcg cgcagcggcc gctcgaagcg
    10081 cggaccctcg ccctcggcaa aattcagccc cagccccatg gcgtcgggta tcgtgaggat
    10141 atcggagaag acgatggcgg catcgagccg gaagcgggcg agcggctgca gcgtgacttc
    10201 ggtggcaagc gccggattct tgcacaacgc gagaaagctg ccggcgcgcg ctcgcgcctc
    10261 gttgtactcg ggaaggtagc gcccggcttg tcgcatcagc cagatcggcg tgtagggagt
    10321 gggctgcctg aacaacgccc ggatcagcgt gtcgttcttc agcttcgtca tggtgaggtg
    10381 tgaagtgtga ggtgtgagga gccgaagacg ccggccgggc gttgcttacg cctcgctctt
    10441 caaccctcac tgctcaccga ggcagctccg agctgcccat caagaattcg tccaccgcgc
    10501 gtgcgcactg tcgcccctcg cggatcgccc ataccacgag cgactgcccc cggcgcatgt
    10561 ccccagcggc gaacaccttg ggcatcgaag tcgcgtagca gccggctcca tcggtagtcg
    10621 cattggcgtt gccgcgggcg tccttctcca cgccgaaagc gtccaggatc gactgcaccg
    10681 gcgagacaaa acccatcgcg agcagcacga gatcggcggg cagcgtgaac tccgagcccg
    10741 ctatctcgct catcctgcca tccctccact cgagccgcac ccccctgagc gccttgacct
    10801 tgccaccctc gccgatgaac tccttggtgg cgaccgacca gtcgcgcatc acgccctcct
    10861 cgtgcgagga ggaggtgcgc agccgcgtcg gccagtaggg ccagacgggg ttcctctcta
    10921 cgtcgggggg catgggcagc agctcgaact gcgtgacgga ccgggcctcg tgacggtttg
    10981 aagtgccgac gcagtcggaa cccgtatcgc cgccgccgat gatcaccacg tgcttgccgg
    11041 tcgcccggag gtcgggcacg cctgtgtcgc ccgcaacgcg cttgttctgc agcgggagga
    11101 attccatggc gaagtgcacg ccatcgagat cgcgcccggg aatcggcaga tcgcgcgact
    11161 gctccgcgcc gccggcgagc accacggcgt cgaactcggg aagcagcttc tcgggcacga
    11221 tcatctcggt cgcccagtcg ggaacgccct ttccggggcg ctgatttccg acatagacgc
    11281 cgacgcggaa cgaaacgccc tcggcggcca tctgctccat gcgcctgtcg atctgccact
    11341 tctcaagctt gaaatcgggg ataccgtagc ggagcagccc gccgatgcgg tcgttcttct
    11401 cgaacaccac cacgtcgtgg ccggcgcgcg ccagctgctg cgcgcaggcg agcccggcgg
    11461 ggccggatcc gaccaccacc acctttttcc cggtccgctg cgccgccggc tgcggcttga
    11521 cccagccccg ctcccaggcc ttgtcgatga ttgcgtgctc gatcgacttg atgccgaccg
    11581 gatcctcatt gatccgcaac acgcatgcct cctcgcaagg cgcgggacac acgcggcccg
    11641 tgaactcggg gaaattgttg gtggaatgca gcgtgtcgat cgccgccttc cagtcctggc
    11701 gaaagacgag atcgttccag tccgggatga tgttgttgat cgggcagccg ctcatgcaga
    11761 acgggatgcc gcagtccatg cagcgcgcgc cctggatctt cgcctgctcg tcggtcagtg
    11821 gcgggatgaa ttcgcggaag tgcttcttgc gccggtcttt cgcctcgtag gcctcttcga
    11881 cccgctcgaa ttcaagaaat ccggtaactt ttcccatgca atctttcggc gtgccccgcc
    11941 gctcgggacg gggctggagc cgctgcgttg tcagcggggt ttatgcgacc agccggcgtt
    12001 gcttggcggc aagctcgccc agggcgcggc ggtactcgtg cgggaacacc ttgacgaact
    12061 tcggccggaa ctcgctccag cgctcgacta tgcacttcgc ctgcgcgctg ccggtataac
    12121 gggcgtggtt ctcgatcagc cgcttcacga tcagctcgtc ggcgacgccg ccgtgccaca
    12181 agtcgcgtac gagcttggca tcctgctcgg cttccgagag caacggctcc aacgcaacca
    12241 tcgaggtgtt gcagcgcctg gagaattcgc cgtccacgtc gagcacgtag gcgatgccgc
    12301 ccgacatgcc ggcggcgaaa ttgcggccgc tcacgccgag caccaccacg ctgccgcccg
    12361 tcatgtactc gcagccgtgg tcgcccacgc cctcgacgac cgcttgggcg cccgagttcc
    12421 tcaccgcgaa gcgttcgccg gcgacacccc ggaagtacgc ctcgccgcag atcgcgccgt
    12481 agagcacgac gttccccgtg atgatgtttt cggtcggctc gccgcggaac ttgggcgagg
    12541 gctgcaccga aatgcgcccg cccgagagac ctttgccgca gtagtcgttg gtgtcaccga
    12601 tcaagtcgag cgtcacgccc ttcgccagga acgcgccgaa gctctggccc gcggagcccg
    12661 cgaggcggat acgaatggtg tcatccggga gtccctcgtg accgtatcgt ctggcgatct
    12721 cccacgacag catcgttccg accgtgcggt tgatgttgcg gatcggcgtc tcgatcgtca
    12781 ccttctcgcc gtgctcgagc gccggccggg cgagttcgat caaccgcctg tcgagcacct
    12841 tttcgagccc gtgatcctgt tgctcgcggt ggtagcgtgc gacctcggct ggcatctgcg
    12901 gcacctggaa gatcttggag aaatcgagcc ctttggcttt ccagtgctcg atacccttct
    12961 gcacgtcgag caggtccgag cgcccgatga gatcgttgaa tcggcggatg ccgagcttcg
    13021 ccatgagttc acgcgcttcc tcggcgatga agaagaaata gttgacgacg tgctcgggct
    13081 tgccctcgaa tttccggcgc agcacgggat cctgggtggc cacccccacc gggcaggtgt
    13141 tgagatggca cttccgcatc atgatgcagc cctcgaccac gagcggcgcg gtggagaagc
    13201 cgaactcatc cgccccgagc aacgcgccaa tgaccacgtc gcgaccggtc ttcatctggc
    13261 cgtccacctg taccgcgatc cgcccgcgca ggcggttgag caccaacgtc tgctgggttt
    13321 cggcgagccc gagttcccag ggcgtgccgg cgtgcatgat ggatgaccag ggcgaggcgc
    13381 cggtgccgcc gtcatggccc gcaatcgtca cgtgatcgga cttggctttc gagacgcccg
    13441 cggcgaccgt tccgacgccg acttcggaga ccagcttgac gctcaccgcc gcctcgggat
    13501 tcgcgttctt caggtcgtgg atcaactgcg cgatgtcctc gatcgagtag atgtcgtggt
    13561 gcggcggcgg ggaaatcagc tcgacgccgg gggtcgaata gcggatctcc gcgacgtact
    13621 ccgagacctt gcgcccgggc agctgcccgc cctcgccggg cttggcgccc tgcgcgatct
    13681 tgatctgcat catctcggaa gaggccaggt actcggcggt gacgccgaag cggccggagg
    13741 cgacctgctt gatcttggac ttgagtgaat cgccctcctc gagcgggatg tcgacttcga
    13801 cgcgctcgcg gcccaggcgc gacaggagcg tttcgccggc tgcgactttc gcgtagcgct
    13861 tgcggtcctc gcccccttcg ccggtattcg acttgcctcc aatccggttc atcgcgatcg
    13921 cgagcacggt gtgcgcctcg gtcgagatcg agcccagcga catcgccccg gtggagaagc
    13981 gcttgacgat ctcggctgcg ggctcgactt cctcgatcgg gagcggcttg catcgttcga
    14041 acctgaactc gaacagcccg cggaacgtca agtagcgctt tgactggtcg ttgatgagcg
    14101 ccgcgtattc cttgtaggtt gagtaggagc ggttgcgcgt cgagtgctgc agtttcgcga
    14161 tcgcatcggg cgtccacatg tgctcttcgc cgcgcacgcg ccaggcgtat tcgccgccga
    14221 catcgagcgc gtctcggagc accgggtcat cctcgtcgaa cgccgcccgg tggatgcgga
    14281 tcgcctcctc ggcgacctcg aacacgccga tgccttcgac cgatgatgtc gtgccggaaa
    14341 aatatttgtc aacgagggag cgcgccagcc ccaccgcctc gaaaatctgc gcgccggtgt
    14401 aggacatgta ggtcgagatg cccatcttgg acatcacctt cttcaggccc ttgcccaccg
    14461 ccttgatgaa gttcttgcac gccttgccgg catcgatgcc gggcccgagt agccccttgt
    14521 ccgcgagctc gatcaacgtc tcgaaagcca gatagggatg caccgcctcg gccccgaagc
    14581 cgccgagcag cgcgaagtgg tggacctcgc gcgccgaacc ggtctcgacc acgaggccgg
    14641 tcgaagtgcg caggcccttg ttcaccaggt gctggtgaat cgcggagagc gcaagcagcg
    14701 cgggtatcgc gacccgctca cggtccatct tgcggtcgga gatgatcagg atggaatagc
    14761 ccgagcgcac cgcgtcctcg gcctgggcgg cgagggaagc caggcgcgcc tcgaccgcct
    14821 gcttgcccca ggcaaccggg taggcgatgt cgagctcgaa cgagcggaac ttgccatcgg
    14881 tgtatttctc gatgtaccgg atggtctcca tctgctcgaa atcgagcacg ggctggctca
    14941 cctccagccg caacggcggg ttcatctcgt cgatgccgag caggttcggc ttcgggccga
    15001 tgaaggagac gagcgacgtc accagctcct cgcggatcgg gtcgatggcg gggttcgtga
    15061 cctgcgcgaa gagctgcttg aaataatgga acagtgtctt gtttttccgc gacagcaccg
    15121 caagcgcggc atcattgccc atcgaaccga ccggttcctc gccgttggtt gccatcggcg
    15181 cgagttgaaa cttgacgtcc tcctgcgtgt aggcgaaagc ctgttgccgg tcgagcagcc
    15241 gctccttcga ggcttcgcgt ttttccgacg gcgcgggcag ctcgtcgagc ttcacccgga
    15301 tcctctcgat ccattcggca tagggcctgg cggaggcaag cgtgtccttc aactccttgt
    15361 cgtcgatgat gcggcctttc tcgaggtcca ccaggaacat cttgcccggc tgcaggcgcc
    15421 acttcttgac gattttttcc tctggcaccg gcagacagcc gcactccgag cccatgatga
    15481 cgagatcgtc gctggtgacg atataacgcg aaggccggag gccgttgcgg tcgagcgtcg
    15541 cgccgatctg gcgcccgtcg gtgaacgcga tcgaggccgg accgtcccac ggctccatca
    15601 tcgccgcgtg gtattcgtag aacgcgcgcc gcgcggggtc catcagcgta tggttttccc
    15661 acgcctcggg gatcatcatc atcatggcgt gggcgatcga atagccgccc atcaccagga
    15721 gttccagcgc attgtcgaac gaggcagaat ccgactgacc gtcatagatc agcggccaca
    15781 ccttgtcgag atcgcgcccg agtatcggcg aggagatcgc gccctggcgc gcgcggatcc
    15841 agttgatgtt gccgcgcagc gtgttgatct cgccgttgtg gcagatcatg cggaacggat
    15901 gcgcgaggtc ccagctcggg aaggtattgg tcgagaagcg ttgatgcact aacgcgagcg
    15961 ccgactcgac cctggcatct ttcaggtcga ggtagtactc gcccacctgg taggcgagca
    16021 gcatgccctt gtagcagacg gtgcgcgcgg acatggaggg cacgtagaac tctttgccgt
    16081 gcacgagctt gagcgcctgg atcgcgtggc cggcgctctt gcgaatgatg tagagcttgc
    16141 gctccagcgc gtcggtgacg gtgatgcctc ggccccgacc gatgaatacc tgccggatca
    16201 cgggctcgat acgctgcgcc ggctcggcga gatcggtatt gtcaaccggc acgtcgcgcc
    16261 agccgagcaa cacctggcct tcgtccctga tcgcgcgctc gatttcgtac tcgcacgcaa
    16321 gacgcgaggc gggatcgcgc ggcaggaaca ccatgccgac gccgtattgg cccaccggcg
    16381 gcagcttcac ggcgcgcttg gccatctctt cgcggaagaa ccggtcggga atctggatca
    16441 ggcagcccgc gccatcggag agcttagggt cccagccgac cgcgccgcgg tgcgtaaggt
    16501 tgcgcagcac cgtcaggcct tgctcgatga tcgcgtggct tttcttgccc ttgatatgcg
    16561 cgacaaagcc cacgccgcat gcatcgtgct cgtgcgctgg gtggtacagc ccccgctgtg
    16621 acggcaggcc accttggttc cagagctcgt tcatgaaaaa gtcgttacca caatgggttg
    16681 cgatcaaata tgctgcgctg ccgcaaacgc gccagaaagc acagcttaac tattaagttt
    16741 actggcttaa atagttggac tcaaccgaaa attgcattgt gcgcgcattc ggtcaggtct
    16801 cctcgatcga gaacagccgc cggtgctcga ggatcgcata gcggtcggtc atgccggcga
    16861 tgtaatcggc gatggcgcga gagtggtcgt tcagcgcgcg cgcctggaac tccgggggca
    16921 gcagacgggc gtctgacagg aacacgccga acagctcggt gacgatacgg cgcgccttgg
    16981 cgctcatgcg caccacgcga tagtggcggt agaggttctc gcgcagaaaa tgcttgagct
    17041 cccggctctc ctcgcggatg cgcgggctga aggtgatgag cggtggcgcg agacgcactt
    17101 cctcgatcga tcccggggca tgacggcgga tgttttcagc gctggcgcgg accagatcgg
    17161 tcaccagggt atcgatcatg cgccgcaccg tctcgtgcac caggcggcgg cctgtcacct
    17221 tggggtgttc acgcaacacc gccttcaggt ggcgcgcgac gattctcacg cccgcgagct
    17281 gatcgaggct caacaggccc gagcgcagtc cgtcgtccac gtcgtggttg ttgtaagcga
    17341 tctcgtcggc gaggttggcg atctgcgcct cgagcgaagg gcatcgcttc ttcacgaagc
    17401 gttcgccgac gtcgcccagc gtccgcgcat tcctcaggga gcagtgtttc agcacgcctt
    17461 cgcgcgcctc gaaggtcaga ttgaggccgt cgaaggcggc gtagcgctgc tcaagaatat
    17521 ccacgacacg caggctctgg agattgtgct cgaatccccc atagggtctc atgcaggcgt
    17581 tgagcgcatc ctgtccggtg tgaccgaagg gggtatggcc gaggtcatgc gcgagcgcga
    17641 ccgcttcggt gagatcctca tcgagccgca agctgcgcgc gaccgagcgg gcgatttgcg
    17701 ccacttcgag gctgtgggtg agacgggtcc ggaacaggtc gccttcgtgg ttgatgaaga
    17761 cctgggtctt gtattcgagg cgccggaacg cggtggagtg gatgatgcga tcgcggtcgc
    17821 gctggtattc gctgcgtccg cgcggcgcgg gctcgcggtg gcgccggccg cgggagtgct
    17881 caggccgcgc cgcgtagggg gcgagttcgc gatcaggcat gcgcccgcgt cgcgccgcgg
    17941 agcacctccg caagcgccgc gcccgggacc tgatcggaaa cgatggcctc gccgatgcgc
    18001 ttgagcagca cgaagcggat gcggccgcct tcgatcttct tgtcgtggcc catcaactcg
    18061 aggtagcgtt ccaggccaag atcgggggcc tcgacgggca ggccggcgac cgccaggatg
    18121 gatgcgaccc gccccgcctc ttccttccct atcaagccga gccgatgcga gagccgcgcc
    18181 gccagtgtca tgccgcttgc cacggcttcg ccgtgcagcc acgcgccgta acccgttccg
    18241 gcttcgattg cgtggccgaa agtatggccg aaattgagca acgcgcgcgg gccgctttcc
    18301 cgctcgtcga gggcgacgac ttcggcctta ttggcgcagg agcgctcgat ggcgtaagcg
    18361 agggcctcgg gctcgcgcct gacgagccgc gccatgttgg cttccagcca atcgaagaat
    18421 tcccggtcgc ggatcagccc gtacttgatc acctccgcca gcccggcgga gagttcgcgc
    18481 gcgggcaggg tattgagggt gtcggtatcg gaaatgaccg caagcggttg gtagaacgcc
    18541 ccgatcatgt tcttgcccag cgggtgattg atcgccgttt tgccgccgac tgccgaatcg
    18601 acctgggcga gcagcgtagt cggcacctgg agataaggca cgccgcgctg gtagatcgcc
    18661 gcggcaaagc cggcgaggtc tccgatcacc ccgcccccca gcgcgatcag cgcggtgctg
    18721 cgctcgcagc ggtgctcgag cagggcgtcg aatatgcggt tgagcgtctc ccagttcttg
    18781 tacgcttcgc cgtcaggaag cgtaatggac acgacctcca ctcgttccgt ctccagcgcg
    18841 cgcgtgagcg gcatgaggta gagcggggca acggtggaat tggtgacgat ggccgctttc
    18901 ctgtgccgca acctccccgc gatcaacgca gcgcgcgtga gcaggccggc accgatgtgt
    18961 atcgggtagc tgcgttcgcc cagttccacg ttcagggtct gcatggctct ttttgggcat
    19021 cgccgctcag ccggtgcgca gcggcgcgtt ctcctgccgg ctcgcatcgg cgtggtgccg
    19081 gagcaactgg tgttccagcc tgtgtgtaag gctgcccagg ctctggctgc cggtatcgac
    19141 gacgatgtcc gcgacgctgc gatagagcgc atcgcgctcg tcgaacagct gctgaagccg
    19201 ggccagggga tccgcggtcc tgagcagcgg gcgattctta tcgtggcgcg tgcggctcca
    19261 cagatccgcg gagttcgcgc gcaggtagac cacgaccccg ttttcggcga gcagcttgcg
    19321 attgtcggct gagagcacgg ccccgccgcc ggtggcgagc acgatctccc tgcgccggac
    19381 cagctccgcc aggattttcg cctcccgcgc gcggaacccg gcctcgcctt cgatctcgaa
    19441 gatcaccgcg accttgaccc ccgttgcgcg ctcgatctcg tggtcgcaat catagaaggt
    19501 cttgccgagg cgtcgggcga gcagcttccc gaccgaggtc ttgccggcgc ccatcaaccc
    19561 cacgagaaag atgttaccgg gtacgcgccc ttgcccggca gccgttttct gtgcgtcgtc
    19621 gcccatgggg cgattctagc ggaacggagg ggagccgcat aacctgcgcc cggcacagga
    19681 gcggcaataa aaaaggcggc cgtggcggcc gcctttttcc gggacgtgtc cctcgcctac
    19741 tggcgcagcg tcagccggtc cttgatgatc ctcggcgtga tgaagatcag cagctcgttg
    19801 cgattgtccg acttcaggtt ctgcttgaac aggaacccga cgtatggcag atcgcccagg
    19861 accggtatct tgttggtcgt ggaggtttcg gtctgggtat agattccgcc gatcaccacg
    19921 gtgccgccgt tttccaccag cacctcggtc acgatctgct tggtgtcaat cgacggcccc
    19981 gacagcgtat tttgccccac gctgtccttg tggatgttga ggttcatgat cacgttgtcg
    20041 tccggggtga cctggggctt gaccttgagc gagagcgtcg ccttcttgaa ggagacgctg
    20101 gtcgcgccgc tcgaggtggc ctgctggtac gggatttccg tgccctgctc gatggtggcc
    20161 tccacctggt cggcggtgat cacgcgcggg ctggagatga tcttgccgcg gccgtccgcc
    20221 tgcagcgccg tcagctcgag gtcgaggaac ttcgtgaggc tctggttgaa caggatcacc
    20281 gagaacacgc ccgggctcgc cccgccgaga cctggaaccg gaaggttcac gttgaggttg
    20341 ccggacgaga agggagctac cgccgctgtt cccgccagcg gcgcgagcgt cgtcgcgctg
    20401 ccgccgacgg ttacgcgcgg agctttggag gagggtgagg acgtgatttt gctgccatca
    20461 gcctcctccc ggtagccgag cctcacgccc aggttgcggc tgaagttatc ggttgcctcc
    20521 acgatgcgcg actcgatcat cacctgccgc accggcacgt ccaccttgcg gatgagcttg
    20581 cgcacctcct ccagccggct tggcgtgtcc tgcacgaaga tcgtattggt gcgcgcatcg
    20641 accacggcgc tgccgcgctt ggacaggatc ttctggttcg ggtcagagag cagtttctgg
    20701 aacgcatccg ccttctggta gttcaactgg aagctctcgg tgcgggtggg ctccaggtcc
    20761 gtgatctgct ggcgcgcctc gagtgccagc ttctcgcgcg tggcgagctc gtcccgcggc
    20821 gcgatccaca ccacgttgcc ggtcttgcgc atgtcgaggc ccttgctctg caggatgatg
    20881 tcgagcgcct ggtcccacgg cacgtccttc agtctcaacg tcaggttgcc gcccaccgtg
    20941 tcgctggtaa tgatgttgag accggtgaaa tccgcgatca cttgcagcac ggcccgcacc
    21001 tccacgttct ggaagttgag cgagagcttc tcgccggtat agccgccgcc gcgcgcgatg
    21061 cggtcctggt cctgggtttg ctgcttcact tccacgatga accgggtgtc ggattggtag
    21121 gcggtgtgct cccaggcgcc ttgcggctcg atcgtcaccc tcgtgttgct gccttgggtg
    21181 aaggcatcga tggtctgcac cgcggtgccg aagtcggtca cgtccagccg cctctgcagg
    21241 ttcttgggca gcgcggtgtt gatgaagtcg acgacgatgg tcttgccctg cagcttgaga
    21301 tcgatgccaa cggcgttgtc ggaaagatcg atcacgacgc gcccttcgcc ggcgctcccg
    21361 cgacggaaat cgatgtcacg cagcgcgtga cgccggtcgc ccggctgcgc ttcggcgaaa
    21421 tgggtggtga cgcttgcggg tccctcggca gcagcggcac cctgcagcgt gatgagcagc
    21481 gcgcgcccgt cgatttgggt gtcataactg gtagcgcggg tgaggttgag cacgagccgc
    21541 gtgcggtcgc ccgcctgcac cacgttgatg ctgcgcaggt cgccctcgcc cacttcttgc
    21601 gtgctgcggc cgagcgcgct ggtggtattg gggaagtcga aagcgatgcg cggcgggttg
    21661 ttgacggtga acccggcggg cgggctggct ggcgcttgct tcagcgtcac cctgacgact
    21721 accttgccgc cggtcgcggg cgatacgttg atcgcctcga tggtgttcgc ctgggcgcgg
    21781 gcggcaccgc tcgctaccgc gagcagcagc cccagcatcc cgggcagtat tgtcgaaaaa
    21841 atagaaaagg cgcgaagttt cattggtgcc tcctgtcatt cctgcagcag cagtgttcgt
    21901 tcttcttcct tccactcatt gccactgtcc tgaatcagct ccttcaattt caccgcggat
    21961 tcgctgatgt cggtaatgcg cccgaagttc tgtcccatga agtccccggt ctggacccgg
    22021 tacaagttgt tgtccggcgt cttgatcaac gcgaagaatt ccttcttctg ctgcagcgtg
    22081 cctaccatct tgaggttttc cagcgggaac gcttccagcg gccccttgcg ccggttgggg
    22141 tcgaacacct tggggtcgat cgtggggctc ggtggacgtt tcggcgcctc gatcttgcgc
    22201 ggcacgaagg gatcgacgag gtcgaacgcg gcgtattcga agggttcgta cggcttcact
    22261 tccggcagag gcggtatgcg gccgcgcggc atgctgtccg aatccttgac gaattggcgc
    22321 aagtccgaat gctgctcgcc gctgcacgcc gcgagcgcaa ggcaggaaag cgagatggcg
    22381 aggatgtaac gtgtcatttc ttgcccttcg cagcgggagc agccttcttc ctctgttgcg
    22441 cgatctcatc ctcatcgaga tagcggaagg tcctggccgt cgcatcgagc accagccccc
    22501 cgcccttggc gccgccgggg gtaagcccga catcgttcaa cgtgacgatc cgggagagct
    22561 tgctgatgtc gctggcgaag gcgccgatgt cgtggtaact gccggtgatt ttgatcgtga
    22621 tcggcagctc cgcgtaaaaa tcccgcctgg tttcgctggc ggcgggcttg aacagctcaa
    22681 actgcagtcc ccgtcccagt ccggcttggt tgatatcggt cagcagcgca tccatctcgg
    22741 acttgcccgg gagctgcttg agcaatgcac cgaattgttt ttcaatgtcc tcaagctgct
    22801 tctggtaagc cggcaggttg atcgcctcgc tcttcttggt gaggaatgtc ttgcgcaacc
    22861 cttcttcctt ctgggcctcg gccttgatcc ggttaatctg gtcgtcccag tcaaaccagt
    22921 agctcacgcc gacgatcacc acgagcacga gcagcagcac cacgagcttg ggcaggatcg
    22981 gccagctgcc gatcttcttc gggtcgagcc gtttcaaatc gtcgagtgtc atggccatgc
    23041 cgcatcaccc cttcgctgcc ggcttgggcg ccggtttctt ggcggcgtcc tcgaccttgg
    23101 cgcgcgtgat attgacgttc atgatgaact cgttgacgcg ctgccccctg tcctgcgccg
    23161 ccttgatctc gatcagcccg ggactttcca ggcggggcga ggattccagg ttgcgcatca
    23221 gcgtggaaac ccgcgcctgc gactgggtga aaccgctgat cgtgatccgc gtgccgacct
    23281 gcttgatgct cttcaggtag acgccatcgg gcagctgccg caccagctga tcgagcaggt
    23341 ggaccacctc ggagcgggtg gcctgcaggg tttccaccac ccgcttgcgt gccagcagcg
    23401 ccgcggtctg gtccttcagc ttctggatct cggcgatctg cttgtcgagt ttctcgatct
    23461 cgctctggag gtacttgttg cggctcacct gattgttgaa ctggtcctcg agataggtgt
    23521 gcacggcaaa ccagatgacg gcgccgatga cgaccacgct gcccagcagg atgaagaact
    23581 gctgctgctg ctgcttgcgc cggatttcgc gatgcggcag gaggttgata cggatcatgg
    23641 gtcaaacctc cgcatggcaa gcccgcacgc caccatgagc gaaggcgcgt cctgcgtcag
    23701 ggacttcgcg cggatcttcg aggacagcgc catgttgacg aacggattcg cgaccatcgc
    23761 gttgacctgc acgcgcttcg ataccgcctc gtctacgccc ggaatcgcgg cacacccccc
    23821 cgagagcacg atatggtgga tctggttgtg ctgggtcgag gtgaaaaaga attgcagcgc
    23881 ccgcgccacc tccaggccca gcatgtccat gaacggttgc agcacgtcgg actcgtaatt
    23941 ttcgggcaat cctccggcac gcttggcggc ctcggcctct tcggggctca acccgaatcg
    24001 gttctggatt tcctgggtga gctggttgcc gccgaacggc tgttcgcgca tgtagatcga
    24061 ttgatcgttg cgcagcacgt tgaccgccat gatggtggca cccacgtcca caatcgcgat
    24121 attctggtcc ttgcccttct cggaaaactg ggtttcgatc agctcgaagg cggtctgggc
    24181 ggcgaatacg tcgacgtcca tcaccaccgc cttgagtccc gccgcctcgg cagcggccac
    24241 ccggtcgatg atcttgtcct tgcgggaggc ggcgatcagc acctcgacgt cttccgcgct
    24301 gttgggcgcc gggccgagca cctggaaatc gagattgact tcttccaggg cgaaaggaat
    24361 gtactgattg gcctcggttt cgacctgcag ctcgaggtct tcctcggttt gtccgcccgg
    24421 caccaggatc ttcttgctga tgacggcggc attgggcagc gcgagtgcga ggttcttgat
    24481 gttgctgccc agacgcttgt ggcagcgctt gagggcgtcg ctcaccgcgt caaggttgtt
    24541 gatgttgccc tcgaccagcg agtccttggg caggggctcg atggcgtagc gctcgacgcg
    24601 atacatcccc ttgccggcgc cctcgatctc caccatcttg atcgccgagg aactgatatc
    24661 agccccgatc aacggcggca tcttggcctt gaacaggccc tcgatggcgt cgaggttcag
    24721 actaaatttt cctttgagca taggttaatg gggagacaat cccacagttt actttaaatg
    24781 ctagcaacaa ccattttgga tgtaaagcgt tttttcccgt ttttatattg gtgcggaacc
    24841 ccgcttataa tggaggggca ctaaatatag cttactctgc aacaagttgc tgcagtgtgg
    24901 caggatgcac aagctgatca ccaattcccc tttattgttg caatactgca acaataagct
    24961 attaaagtat tgatttaatg ccgattcgct ggttagcctt tcctctactc ctggtgcttt
    25021 ccagcattct ggcggcgttc atattggctg catttgctgc ggtcatcgtc tatcccacac
    25081 tgccatcgct ggagatgctc actgactatc agccaaaaat accgctgcga gtctacagcg
    25141 ccgacggcaa gctgatcggc gagttcggcg aggagcgccg atccgtagtg aaaatacaga
    25201 atgtccccga gcccatgatc cgtgccattc tcgcggcgga ggacgagcga ttctatcagc
    25261 accacggcgt ggactacgtc ggcgtggcgc gcgcggcgct gtccaatttc atgtccggcg
    25321 gtgtgcgcca aggggcctcc accatcacca tgcaggttgc ccgtaacttc ttcttgtcca
    25381 aggagaaaac gctgacccgc aagttcaacg agatgctgtt ggccttcaag atcgaagcca
    25441 atctcagcaa ggaccagatc ctcgagctct atatcaacca gatttacctc ggccagcgcg
    25501 cctatggctt tgccgccgcc gcgcaaatct atttcggcaa acagttgtcc gagttgacgc
    25561 ttgccgaatc ggcactgctc gcagggctac ccaaagcgcc ttcgcgctac aacccgctcg
    25621 ccaaccccaa gcgcgcgaag ttgcggcagc aatacgtgct gcgtcgcatg cgcgagctgc
    25681 aattcatttc ggaagaagag cttgcctccg ccgaccgcca gccggtactg gtaaagaagg
    25741 aaatcaacga ctacccggtt cgcgccgagc atttcgcgga aatggttcgc caggccatct
    25801 acgagcgtta tcaggaggac acctacacca agggcatccg cgtctacacc acccttctga
    25861 cctcgcacca ggaggcggcc tacgcggcga tgcgtcgggg cgtgcaggag tacgaccggc
    25921 gtcacggcta tcgtggagcc gaaggatatg tcgaattgcc cgccaaactg accgatgaaa
    25981 cactggaaga tgcgctgcag gagaccaccg acagcgacga cgtctacgcc gttgtggtca
    26041 ttgaggccgg ccccaaactg gtacgggcat atcgcaaggg cggagagtgg gtggagatca
    26101 aggaagaggg gctcaagttc gcagcccgca tgctgggcga gaaggcgagc cccggccagc
    26161 gcatccggcg cggcgccatc atccgcgtgc agaaggacga aaaagggcgt tggcagatca
    26221 cccagctgcc tgcggtcgaa gcctcggtca tcgcggtgga ccggcgcgac ggcgccattc
    26281 gggcgctggt cgggggcttc gatttcagcc acaaccagta caaccacgtt acccaggccc
    26341 tgagacagcc aggctccagc ttcaagcctt tcatctactc ggcggcgctc gaaaaaggct
    26401 tcaccacggc gaccgtcatc aacgacgcgc cgctcacctt cacggcggcg cagaccggct
    26461 cagaaccgtg ggagccgaag aattacgaca gcaagttcga agggccgatg cgtttgcgca
    26521 ccgcgctcgt caagtccaag aacttggtct ccgtgcgcat cctgcaggcg atctccccgc
    26581 agtacacgca ggattacatt gcacggtttg gttttgatcc caagctccac cccccgtatc
    26641 tcacgatggc gctcggctcg ggtaatgtga cgccaatgca gatggccacc ggctacgccg
    26701 tcttcgccaa cggcggttat cgcgtggcgc cgtacttcat tgagcgcgtg gaggatgcgc
    26761 gcggcaatgt gctgatgtca gcaaagccgc tggcggcggg cgcgggggcg gaacgcgtga
    26821 tcgacgcgcg caacgccttc atcatgtcaa gcatcatgcg cgacgtcgtg cgcatgggca
    26881 ccggcgcgcg cgcgatgaaa ctcgggcgcg tcgatcttgc cggcaagacc ggcaccacca
    26941 acgagttcgt ggacgcctgg ttctgcggct acggcggcga gctggtggcg gtggcgtgga
    27001 tcgggttcga tacgccgcag acgctcgggc gcaacgagac cggcgcgcag gcggcgctgc
    27061 cgatctggat gggtttcatg ggcgccgccc tgaaaggggt tcccgagcag ccgctcacgc
    27121 cgcctgccgg gatcgtcgca atgcgcgtca acccggatac ggggctgcgg gtggcggacg
    27181 cgcagggagg catcgtcgac tttttctata gcgagttcct gccgcccgag cacgagacgc
    27241 tgattggcgg cgtgatcggc ggcgaccgcc cgcgggaaga agtgcgcaat caactgttct
    27301 gagccgacca tgcccaggga aaccggccgc aacgaccggc gcagccgcat tgcccatttc
    27361 gcggcgcggc tgatggccga ggacggcatc gaggattatg cgctcgccaa acgcaaggcc
    27421 gcgcgccagg ccggcacgcc ggacacgcgc gagctgccga ccaacgagga aatcgatgcc
    27481 gcgctcagga cctaccagca gatctaccat aaagaggagc accgcaaccg gctgcgcgag
    27541 ctgcgcggaa gggcgctgcg ggcgatgcgg gagctcgcgc agttcaatcc ttatttgacc
    27601 ggctcggtgc tgagcggcaa cgccggcaag tatgccgaca tcaatctgca gcttttcacc
    27661 gacaacgcga aggcagtcga actctatctg atcgaccgga ggattcccta ccgggtggcg
    27721 cagagccggc tatattgcgg cgcggagccg ctcacggcgc ccgtctatac cgtcgatgac
    27781 gacggcaccg agatcgagct caccgtgctc tcgacgcgcg aattgcgcgg accgctgcgc
    27841 accagcccgg agggaaagaa catcgaacac gccaagctgc tggtggtcga gcaattactc
    27901 gcgcagacct gatcagccgc ctgccttcag ccagccggcg acatcgaacg catagtaggt
    27961 gaggatgcca tcggcaccgg cgcgtttgaa ggcggtgagg acctccatca cgcatgggcg
    28021 ttcatccagc cagccgcgcg cggcggccgc cttcagcatc gagtattctc cgctcacgtg
    28081 atagacgaac gtgggcgctt tgaactcctc cttgacgcgt cgcacgatgt ccagataagg
    28141 caggcccggc ttgaccatga ccatgtccgc gccttcctcc agatcgagcc cgacttccca
    28201 caacgcctca ttgctgttgg cgtagtccat ctggtaggtg aacttgttgc cgcgcccgag
    28261 gttctttgcc gagcccaccg cgtcgcggaa tggaccatag aatcctgagg catatttcgc
    28321 cgaataggcg aggatgcggg tatcgatgaa ttgttgccgg tcgagggcgg cgcggatcgc
    28381 cccaatgcgc ccgtccatca tgtccgaggg ggcgactacg tcaacccctg ccgcagcgca
    28441 cacgagcgcc tgtttttcca gcaccgcgat cgtttcatcg ttgagcacgt agccatcgcg
    28501 gtcgatcacg ccgtcctgcc cgtgcgtggt gtacggatcg agtgcggcat ccgtgatcac
    28561 tccgagttcg ggaaagcgct ttttcagtgt cgccaccgtg cgcggcacca gccccttcgc
    28621 attggttgcc tcacggccgt cgcgcgtctt gagctcgctc gcaatcatgg ggaacaccac
    28681 cagcgccggg atgccgagtc tagcgcactg ctcggcgtgc gcgagcagcc ggtccactgt
    28741 atagcgccga atcccgggga gggacggcac cgcctcggtc cggttcctgc cttcaattac
    28801 aaaaaccggg taaatgaggt catcggtggt aagccggtgc tcgcgctgca gacgccgcgt
    28861 gaattcgtcg cgccgcatcc gtcgcatgcg ccgccgcgga aagccgccgt acacccgcat
    28921 cgtattaacc ttttgaagag atttgttttt tttgttcccg gggcggggaa ctatcggcca
    28981 ttataatcgt cttaagttgc agacgatgtt gaatcgtctc ccgcttctcc tccctgagcg
    29041 ggcgccttaa tcctagttaa ggcttttgcc cccggtttta ctccccttta accgggggtt
    29101 ttttgtgtac accgtcaggc gttgcgccgc gcaacgccgc tccgaccgga gcggcagggt
    29161 acccagtgaa atggcctagg ttattgttat ggcgtctgaa actggcggct gcggaaaggc
    29221 atactataca atatgcgttc tgctcaataa cggcgtcaat caaggaacct gcatgagctt
    29281 ccgctggcct gtcatgctgc tcgctgccac gctgatttcg ccggccgcca cagcccaagg
    29341 ccccgccaag tcggatcctt cgaaagcgca gccgatcgcg acccaggttt gtggcgcctg
    29401 tcatgccgtt gacggcaaca gcacccaggc cgccaatccc gtgctcgccg gccagcacgc
    29461 cgattacacg ctcaagcagc tcgcgaattt caagccgctg gatggcaagc ccgccgagcg
    29521 gcctcatgcg gtgatggcgg gcatggtggc gaatctctcg gcggacgaca tgcgcaatct
    29581 tgccgcctac ttcgagatcc agaaacccaa gccgcgggcc gcgcgcgacc cggagctggt
    29641 gaagctcggg cgtgcgatct accgcggcgg cattctgtcc aaaaatgtcg cggcttgcac
    29701 cgcctgccat ggtccaaacg gcagcggcgt gccagcccag ttcccgcggc tcgcagggca
    29761 gttcgccgaa tacaccgcgg cccagctcaa ggcgttccgt tccggggaac gtgccaacga
    29821 ccccaatcgc gcgatgcggg cgctggccga gaaactctcc gacaaggaga tcgcggcggt
    29881 cgcggagtac atagccgggc tgcgctgacc cgctgcgcta gctgatcgtg gcaaaaacct
    29941 tccgcgcggc gcctatcgtt ttatcgattt ccgccgctcc gtgcgcgctg gatacaaacc
    30001 ccgcctcgta agccgaggga gcgaaataga tgccctcggc gagcatggcg tgaaagaaac
    30061 ggccaaacgc ctccctgtcg cattgcatca tctcggcgta gctcgtgggc ggtgtttcac
    30121 ggaaatagac gccgaacatg ccgccgacgg atgcggcgga aaagggcatg ccgtgtacct
    30181 gcgcgcaacg ggtgaggcct tcgcataagg agcgcgtgat tgcggtcagt cgttcgaaaa
    30241 acccggaggc ctgtatgagc ttgagcgttg ccaatcctgc ggcaactgcg accggattgc
    30301 cggagagcgt tcccgcctga tagacggggc ccaggggcgc gattttttgc atgatgtcac
    30361 ggcgcccgcc gaaagcgccg accggcatgc cgccgccaat gaccttgccg agcgtggtga
    30421 gatctggttt cacgccgtag agcgcctgcg cccctccgag cgcgacacgg aagccggtca
    30481 tgacctcgtc aaagatcagc acggctccat gccgggtgca aagcttgcgc agcaactcga
    30541 ggaagcccgg atgcggcgcc accaagttca tgttgccggc gaccggctcc acgatcaccg
    30601 ccgcgatttc cgctccttcg cggtcgaagg cgcgttccag cgcgatttca tcgttgtatt
    30661 cgagcaccag ggtatgggcg gctgctggct ccggtacgcc cgccgaggag ggctgtccga
    30721 aggtaagcgc gcccgatccc gccttgacga gcaacgcatc ggcgtgaccg tgataacagc
    30781 cttcgaactt gatgatcttg tcccggccgg tgaacccgcg cgcgaggcgg attgcgctca
    30841 tcgtcgcctc cgtgccggaa ctcgtgagcc gcacctgctc aagcgagggc acgagccggc
    30901 acaggagttc cgccatctcg atctcctgct cggtgggcgc gccgaagctc aagcccctct
    30961 cggcagcctg ttgcaccgcc ttcaccacat cgggatgggc gtggcccagg atgagtggtc
    31021 cccacgagcc gacgtagtcg atataggccc ggccgtcggc atcccacacc tgcgcgccct
    31081 tgccgcgctg gatgaaaagc ggcgtgccac ccaccgagtg gaacgcccgc accggcgagt
    31141 tgacgccggc gggaatatgg agttgcgcgt gctggaacag tttctggctg cgtttcattt
    31201 tgagcgaatg gtatcaattt cgcgatacag ggcgctgaag ccccgtgcgg ccgcccgtac
    31261 gtcgggagcg ccgaacaatg ctgaaatcac cgccacgccg tcggcgccgg cggcaatgag
    31321 ttgcggtgca ttgtcgagcg tgatgccacc gatcgccacc accggcaccg acaggcgttg
    31381 tttggactct tgcagcagtg cgagaggcgc ctgcaccgcg cccggtttga ctgcggacgg
    31441 gaaaaaactg ccgaaagcga cgtagtcaac acccgcgtgt tcagcatcca gcgcgctctg
    31501 catccggttg tagcaggagg ctccgagaat cttgcggtcg ccgagcctgg cgcgcgcagc
    31561 cggcagcgaa ccgtcggcgg agcccaggtg gaggccgtcg gcatcgaggg cgagcgtgag
    31621 atcaaggtgg tcgttgatga tgagcgggac ccggtatttc cggcacagcg cgaggagcac
    31681 gcgcccctgt gtcagccgca acaccgcgct cgcggtcttg ttgcggtatt gcagcaggcg
    31741 cgcgccgcct gcaagcgccg cctccaccct ggcgagcaga tcggacgtgt cgtcggtatc
    31801 gggcgtgacg gcgtaaaggc cggaaatacc gcgcttcaca ccttagccct cgctgcttcc
    31861 accgcccgcc tgctcctcct cgcgcgccca gaacaggcgg tccgggatgt gctggcccat
    31921 gccgggacgg aacgccgctt tcagggtctg ccaggtgtac tcctgggcgt ccttcaccgc
    31981 gtcgctcacc tcaagcccgt tggcgatggt ggcggcgatg gctgaggcca gcgtgcagcc
    32041 cgagccatgg tacgaaccgg gcaagcgctg ccaggtgtcg ctgcgaacca cgccgttctg
    32101 gccataaagg gtgttgacga cttgggtggt gttttcgtgg gtgccggtga ccaggacgta
    32161 ttcgcatccg ctcgcgatga tccgccgtgc gcactcggcc aggtcgaggc cgtcaccttc
    32221 ctcggcttcc tcggccgcga gccgccgcgc ttccaggctg ttgggcgtca ggatggtggt
    32281 ctgaggaatg agcaactcgc gcaacgcgct caccatttca tcggtgacca gctcgtcgcc
    32341 gcggcccgaa gcgagcaccg gatcgagtac cagcggcacc tcggggtaat cggacaccac
    32401 ctccgcgatc gccgtgatct gctcgatgtt gccgagcagg ccgatcttga atgcggccac
    32461 cggcatgtcc tccagcacgc agcgcgcctg gtcggcgacc cactcggcat cgatcggcga
    32521 gacgtcgtcc actcccatcg tgtcctggat ggtgaccgcg gttaccaccg aaagcgggtg
    32581 gcagcccatg ctcgagagcg tcatgatgtc cgcctggagc cccgctccgc cgctcggatc
    32641 ggtcgcggcg aatacgagca cgatgggagg aagatcgggc atgcgcgagg aattcgaaat
    32701 gcccgcacag ttggtggcgg gcggagtgat tcgctagaat ttgattttat cgtaaaacgg
    32761 atgcaggcgt gacgaccacc gaaacccggg tctacaagac ctacatgtgc ctcatctgcg
    32821 gattcattta cgatgaagct gcgggactgc cggaggaagg catcgcgccg ggtacgcgat
    32881 gggaggatgt tccgccgaac tggacttgcc cggaatgcgg cgcgcgcaag gacgatttcg
    32941 agatggtgga gatttagctt tttgagggtt aagtgtttag tgttgagttg tcttgaacgc
    33001 ctcggcgtca gactcgacac tgaacactca aaattcaaca ctcaacgctg aacccatgcg
    33061 tatcctgcac accatgatcc gcgtcggcga cctgcagcgc tcgatcgatt tctataccca
    33121 ggtgctgggg atgcggctgc tgcgcaagac cgactatccc ggcggccgct tcaccaacgc
    33181 gtttgtcggc tacgccgatg aatcgaagac agccgtgctc gagctcaccc acaactggga
    33241 cacccgctcc tacgatcttg gcaccggcta cggccacgta gcgatcgaag tcgaggatgc
    33301 ccatcaggcg tgcgcggacg tgaagcagcg cggcggcaag gtgacccgcg aagccggccc
    33361 catgaagcac ggcaccaccg tgatcgcctt cgtcgaggac cccgacggct acaagatcga
    33421 gttgatccag cgtaagaccg cttaggcggc ggtaccagcg gcggcgtccg caagccgcac
    33481 gaaatcctcg acgcgcaggt cctgcgcgcg cagcccggca tccactccga gccgttcgaa
    33541 gtcccggctt ccgaaatagc ctttcaaggc gttgcgcagg gtcttgcggc gccgggcgaa
    33601 cgccgcggcg acgattttcc cgaacagtgc ttcatcgcac gcaaggtgcg gcgggcgcgc
    33661 gaacggcgtc atgcgcacca ccgctgactc gaccttgggc gcgggacgga aggcgtcggc
    33721 cggcacgttg aggacgtgct ccataccgaa gcggtactgg agcatcaccg agagacgtcc
    33781 gtaatcagag gaagacggtg tggcaaccat gcgctccacc acctccttct gcagcatgac
    33841 gtggatatca cggatcgagc cggcgtgccg cgcaagatga aacagcagcg gcgtggaaat
    33901 gtaatacggt agatttccga ccacgcgcaa cagggatccc agcgcgctga aatcgaattc
    33961 gagggcgtcg ccttcgtgca ccgtgacgcg cgcaacgggg aacgctgccc gcaggcgggc
    34021 ggtgatgtcg cggtcgatct cgatgacgtg caggtgatcg agccgcgagg cgagcgggcg
    34081 ggtgagcgcc cccagacccg gcccgatttc caccatgagg tcgcggggct gcggcgcaat
    34141 ggctgcgacg atggcggcga tgaccgactc atcggtgagg aaattctgcc cgaatcgccg
    34201 gcgcgggacg tggcgcatga ggccgtccat cgcgcccgct tcacgcggcg cgccgcttcc
    34261 tgccgcggcc gccggtggcg agcgcggcgg cgagcctgat cgcttcgacg aggctgccga
    34321 tttcggcgct tccagtgccg gccaggtcga gcgccgtgcc gtggtccact gaggtgcgga
    34381 tgatgggaag gcccagcgtc acgttgacgc ctgcgccgaa gctcgcgtac ttgagcaccg
    34441 gcaaaccctg gtcgtggtac atcgcgagca cgcagtcaca gtccttgagc cggctcggct
    34501 ggaacagggt gtcggcgggc aagggcccca cgagatcgaa gccttcgcgg cgcagtttct
    34561 ccagaaccgg gacgatggtc tcggtctcct cgcgcccgag aaaccccgat tccccggcat
    34621 gggggttgag accggcaacg aggatgcgcg gcctggggac cgagaatcgc tcgatcagat
    34681 cgtgccgcag cacgcgcagc gtgcgttcca ggccctcgcg ggtgatgttg cgtgcgacat
    34741 ccttcagcgc gagatgggtg gtggcgagcg caacccgcat gcctccgccc accagcatca
    34801 tcaccacgtg ttccgcgccc gtatgggcgg cgagatactc ggtgtgaccg gtgaacgcga
    34861 taccggcttc gaggatgacg cttttttgca acggcgcggt gaccatcgcg tcgaagatgc
    34921 cttcgcggca gccgtcggcg gcggtttcca gggtgtgcaa cacgtagcgg ctgttggcgg
    34981 gatcgaggcg cccggggaca gccggtcgcg caagcggcac atggcgcacc ctgagtgcgc
    35041 cggcaccggg cttcgcggct gcagcggtag ggaaatccag cagcttcagc gctacgccga
    35101 ggcgacgcgc gcggctcatc agcagccccc ggtcggctac caccacgatg cgcgccggca
    35161 ggcgccggcg cgcgagttgc acgcagagat cgggcccgat gccggcgggc tcgccagcgg
    35221 tgaccgctat cagcggaatt ttgaattttg agtgttgaat gttgagttgc cgcgcgaaag
    35281 aatgcagtgc gtgaaccgta acttaacacc cgagtaactc aacactcaaa actatctttc
    35341 ttcgagccgg tactcgatgt aagcaaggtc gcgctgctgc cgcagccact cctgatagga
    35401 ttcgtcggac ttgcgcgcgc gcaacgctct cctcgcctcc agccgcaggc gctcgcgcga
    35461 catgtcctcg ttgcgccgct ccagcacctg gatcaagtgc catccgaacg gcgtctggac
    35521 cggctcgctg attccacccg ggttgagcgc attcatggca cgctcgaatt ccggtacggt
    35581 gtcgcccggg gacagccacc ccagatcgcc gcccttggtg ttgcttgcat cctcggaata
    35641 ctggcgcgcc agttccgcga aatcagcctt gttgtcaagg cgttccttga ggctcaccag
    35701 gcgccggcgc gcttcggatt ccgggaccag ctcattggtc ttgatcagga tgtgccgcgc
    35761 gcgtgtctgg cggatgatca ccggtacggt gctgccgcga cggtcgttca gcctcaggat
    35821 gtgaaaaccg ttggcgctgc gtaacagggg gctcgcggcg ccaggttgca tcgttttgag
    35881 cgcgtcaaga aacagcgctg gcaggcgtgc ggcgtcgcgc cagcccatgg tcccgccctg
    35941 cagcgcatcg ggtgcgtcgg aaaagctcgc agcgacctgg cggaagtcgg cgccagaccg
    36001 gagttgctcg agcgcctgct cggcgcggct gcgacgggcc tggatctgct cgggactggc
    36061 gttttccggg acggtcacca ggatgtgtga aaggttgaac tcttcgccgc gctcggtctg
    36121 cgcctgctgg gtgttgatga agttgtcgat ctcgctttcg gtcacgacga tccggttgtc
    36181 gacttcgcgc tcgcgcaacc gcgccaggat gatctcgttg cggatgtcct cgcggaactt
    36241 ggagaaggaa acgccgtcct tttccagcgc cgcgcgcagc ccctccagag tgagcctgtt
    36301 ttcctgcgcg atgcgctgga tcgcgcggtc gagctcggta tcgtcaaccc gcaggccggt
    36361 ttccttggcc agctgcaact gcacgcggtt gatgatcagg cgttcgaggg tctgtctctc
    36421 caaagcttcg cgcggcggcg gcggcgtgcc ctgttgcttg agttgagccg cggcgtggcg
    36481 ggtgtgctcg tccaggtcgc cgcgcgtgat cacctcgttg ttcaccaccg ccacaatgcg
    36541 gtcgagcacg atcaccgtgc gcggctgctg tgcccaggcc gccggcgcaa gcgcgatggc
    36601 gaaggcgagg gcggcgtagc gggatgaact ccctccgaat ccgatcatgg aaccgcggac
    36661 ggcatcagta atacggtgac cgtgattcat cgggtctggg ggcgcgggga tccagtctga
    36721 catagccgcc gatgttgcgc ttgagcgttt ccatcggatt ggacccgata cgcgacactc
    36781 cgtttagttc gagctggata aaaaacgtgg tggacgtctg ctgggtagtg gtggcgaagc
    36841 ggtgcgcgac tacgcgcagc acccagcagt cgccattata ttccgcgccg agcaatccct
    36901 cgagggtgcg tcgctcgcgc agcgagtaat tccagcgcgc gagcgccgtc cacccctgtg
    36961 cgaccggcca ttgcgtcgaa acgtcggtct gccgtagcac gttgagcgtc tcgcggtagc
    37021 tcaagttgag cactttgccg ggctgtggct ggtaacgggt gccgacattg aatctctggg
    37081 tctgggaaaa attggtgctg tactgccagc ccgcctcggc tgtccagccg ggcaccaccg
    37141 ttccggaggc cgcggcgagc aggtcggaac tggaactggt gcgcacggcc acgccgggca
    37201 ggaagacgcg ctgggtatcg aagtaatagc gctgcgcgac gccaacgcgc agccgctctg
    37261 cgccggtagc ggccccgatc aggcgcgagg tgacgccaag cgtcacctga ttggcgtcgt
    37321 tgatgcggtc ccatcccgaa aattggttct cggagtaaat ggtcgcgaaa ttgatgtcct
    37381 gctgtccgct gtcaaacacc ggtatcaggc tctggtcacg gaacgggatg taaacgtaat
    37441 agagccgggg ctccagggtt tgcaatacgg ccgttccgcc gaacgaagtc ggccgctcga
    37501 acacgacgcc gctgtcggtg gtgaagatcg gcagcgttcg ggaccggtcg gtaacggctg
    37561 cggcgttcgg gtcgatggaa taatgggtca cgttcaggcc cagtttgggc gtgacgtggg
    37621 cataggcggt ttgcagcggc aggctcatgc tcgggtaggc caacgtgcgg ctgccggtca
    37681 cattggtcgg gtgatcaaat ccgacatagt gtccgtagaa atcgaaatcc gagtatgcga
    37741 tgtcctcgcg tgtggcattg agcgtcaact gcggcaggcg cgcgtagggg gggatgaccg
    37801 gcaccagcgg gtcggtctgc agcgcctgcc agcgttggaa cagggcgttg aagccataag
    37861 taccggcgct gccccaggtt ccgcccctgc cgatcgagcc ctcgcgcggc agcgccgttt
    37921 gcgatgtcaa tgcgatctgc gtcgagaggt cggtgaaata gttgccatcc gaggcgccct
    37981 gaaggttaag cgtgccgaac cagccgccgg gcatggtctg gttatggcgg atattgtagg
    38041 cccagcgccg ctcgccgctg cgctcacggt cgtcaggcag gacatccgcg cgcgcctcgc
    38101 cgaaataagc cggctcgagg tagcggaatt cgttgttgac cagtaccccg cgtttcgtca
    38161 tcacgcgcgg cgagaaggtg gcatcgcggt tgggcgcgat gttccagtag tagggtacgg
    38221 tcaattccgc cccgcccttg tcggtgctgc cgtaatgcgg cgcgaggaaa ccggacttgc
    38281 gcccctggtg cagcgagaac gacaggtacg gcgaatagaa aatcggcatc cccatgaaca
    38341 cgatgctggc atcatgcgcg acaccgatgt cgcggtcctt gtcgatggcg aggtcgcccg
    38401 ccctcaggta ccagtcgtcg ttgcctggcg cgcaagcggt atactcggag cgttgcgcgc
    38461 ggtagcggtt agacccctcg aacaggatcc gctccgccgt gccgcgcgcg tccgtttcgc
    38521 ggaacagctg ccgctccccg cccaggctgg gactcttgtg cagcgtgtag cgcgagctct
    38581 ccatgaaacc gcgttcggtg cccaggttgt acttcaggcg cgcgccttcc accacgtccg
    38641 cgccccgttc catgcgcacg ttgccctccg ccgtgacctc atcgagcggc ttgtcgtagc
    38701 gcaggcggtc ggcgtacacc gcctcgccgc gcttcctcaa tcgcacattg ccttccgcct
    38761 cggtttcctt ctcgttgtgt ccctgcaacc gatccgcttc gaggaacagc ggcacttcct
    38821 ccttgagcga aggcgggatc aggatcaacg tgggctgcgg cctgagtttc agcccctgct
    38881 gcgcgccggc cgcaagcggc agcgcgcaca aaacggcgaa cgagatgcgg gttatttccg
    38941 gcttgtgcat cgggaaaggc tgcggaagcg aggtgttaaa atcgcacaat tcatcgctga
    39001 aggcaaacga gttgcagccg tcatcggaca gacgattgga gcagttgcgg gggtggctcg
    39061 ctgcgcgcat ttcggagggg ccattcacgg tggagcccgc ttctgcggat gcgagcttcc
    39121 gccgttattt tcgcgtggtc gcggagcgcc attcatggat cgcaatggac gcgccgccgg
    39181 agcgcgagga ctgccggccg ttcgtgcgcg tggcggggct gctgcgtgcg gcgggcgtca
    39241 atgcacccga ggtgttggcg caggacctgg agcgcggctt cctgctgctc accgatttcg
    39301 gcgatacgac ctatctcgcc gcgctcgacg aaaccaatgc ggaacggctc ttcagcgacg
    39361 cgatcgcggc gctcatcaag tggcagctcg caagccgcga gggggaattg ccgccgtatg
    39421 acaagacggt tctcgagcgc gagtgccgcc tgttcccgga gtggtatgtt gcgcggcatc
    39481 tcggtatgac gctctcgact gcacagcagc gcacgctctc cgaaatgctt gcgctcgtca
    39541 tctcgcgcgc tctcgcgcag cccgcggttt acgtgcaccg cgactacatg ccgcgcaatc
    39601 tcatggtggc cgatcccaac ccgggggtga tcgatttcca ggacgcggtg tacggaccga
    39661 tcagctacga cctcgtgtcg ctcacccgtg atgcgttcgt gagctgggag gaggaacgcg
    39721 tcatcgactg ggcggctcgt tactgggata aggcgaagcg cgcggggctc ccggtcgacc
    39781 cggatttcag cggcttctac cgcgatttcg agtggatggg gctgcaacgc cacttgaagg
    39841 tgctgggcat cttcgcgcgc atccattacc gcgacggaaa gcccggctat ctcgaggaca
    39901 cgccgcgctt cctcaactac gcgtgcgcgg tggcagggcg ttatcgagag ctcgcgccgc
    39961 tcgcgcgcct gttcgaccag ctcgagaagc gcgaaccggc ggtcggttac accttctgac
    40021 cggcgcaccc ccctaacgtg aaagcgatga tactggccgc cggtcgcgga gagcgcatgc
    40081 gcccgcttac cgagggcgtg cccaaggccc tgctcgaggc cggcggccgg ccgctgatcg
    40141 cgcacttgat cggccggctc gcgcgcgccg gcttcaccga actggtggtc aatgtctcac
    40201 acctcgccgg gctgatcgag cggactttgg gggacggctc gcgctatggc gttcgcatcg
    40261 cctattcacg cgaggcgcag gcgctcgaga ccggcggcgg cattgcttat gcgctgccgc
    40321 tgctcggaga ggctccgttc gtggtggtca acggcgacat ctactgcgac ttcgacttca
    40381 cgcggctggc tgccgccgtg gctgcgctcg atcccggcac ggcttgcgcg catctcgtgc
    40441 tggtggacaa cccgatgcac cacccgacgg gcgacttctg cctgaacggc gggaaggtgt
    40501 cggcggacgg gccgccgccc cggctcacct tcagcggcat cggcgtttac acgcccatgc
    40561 tattcgccgg cgtcgcgcgt gggacccggt gccagctcgt cgcgttgctc aggcccgcca
    40621 tggcgcaaga gcgcgtgagc ggcgagcatc atcgaggtct gtggatggac gtgggcacgc
    40681 cgcagcggct ggccgacctc aaccggctgc tcgcctccgc gcaggaaact tgacggcctc
    40741 gccgcgggtc gcccgcgggc ttgtttataa tttcaggatg acagccagca tcgacaccgg
    40801 catctaccgg cgacggcggg agcggctcgc ggccgcgatg cgtgccggcg ttgcgatcgt
    40861 gccgaccgcg cccgagcgca accgcaaccg tgacgcgcat tacccgtatc gtttcgacag
    40921 ctacttctac tacctcaccg gcttccgcga gcccgatgca gcgctggtga tcgtcgcggg
    40981 cgcggagccc aagaacatcc tgttctgccg cgacaaggat cccgagcgcg agatctggga
    41041 cggtttccgc tacggcccgg aggcggcgcg cgccgccttc ggcttcgacg aggcgcattc
    41101 gatcgatcag ctcgatgcgc tgatgccgga actcctcgcc gatcgcgatg cgctctattg
    41161 ccatctcggc gcggatgccg cctgggatgc gcgcgtgatc ggctggatca atcgcgtccg
    41221 cgcccgcgcg cgcagcggcg tcaccgcgcc cagcgaggtg aaggacgtgc acgccctgct
    41281 cgacgaaatg cggctcgtca agggcccgga ggagcttgcc gtgatgcggc gagcagcggc
    41341 gatcgctgcc ggcgcgcacc ggcgtgccat gcgcatggcg cggccgggtc gggccgagta
    41401 tgaaatcgag gcggagctgt tgcacgaatt ccggcgccat ggcgcgcagg cgccggccta
    41461 ttctccggtc gtcgcctcgg gcagccgcgc ctgcgtgctg cattacgtgc agaacgacgg
    41521 catgttgtgt gagggcgagc tgctgctgat cgacgccggc tgcgaactcg acggctacgc
    41581 ttccgatctg acgcgtacct ttcccgtcaa cggcagattc agcgggccgc agcgcgagat
    41641 ctacgatctg gtgctggcgg cgcaggcggc ggccattgcc gcggtgaagc cgggcaaccg
    41701 ctgggacgcg ccgcaccgcg cggcggtcga gacgctctct cagggactga tcgacttgaa
    41761 gctgctctcg ggcggccttg ccgaagccgt ggaaaccgag gcctacaaga agttctacat
    41821 gcaccggacc ggccactggc tggggctcga cgtgcatgat gcgggcgatt acaagcgcgg
    41881 gggcgaatgg cgggcgctcg tcccgggcat ggcgctgacg gtcgagccgg gctgctatat
    41941 ccgggggggc gagggcgtgc cgcagcgatt tgcaggtatc ggggtgcgca tcgaggacga
    42001 tgtggtggtg accgaagcag gcgccgaagt gctcacgcag gatgccccca agcgccccgc
    42061 cgacatcgaa gcctggatgc ggcagcgcga tgcctgatta cgacgttctg atcgtgggcg
    42121 gcggcccggt cggcgccgcg gcagccctcg ctctgcgcgg cagcggcctg ggggtggcgc
    42181 tgctcgaggc acgggcgccg caagccgcgc gctcggatcc gcgcccgatc gcgctgtcgc
    42241 acggcagccg gctgatactg gagcggctcg ggatgtggga acggcttgcg ccggtcaccc
    42301 cgatttcccg catccacgtc tcgcagcggg gaggtttcgg cagggtcgag ctcgatgccc
    42361 gcgaagccgg tgttccggcg ctcggctatg ttctggatta cggacggctt gccgcgaggc
    42421 ttgccggcgc gctcgaagcg gaaggcggct gccacacagt acgggccacg gtgagcatgc
    42481 tcgaagccgg gccggaggcc gccctggcgc gcttcgacgc cggtcatgga cccgagcagg
    42541 cgaccgccag gctgctcgcc gtggccgacg gcggcgcgct aacggtggca cggatgcgca
    42601 cccgggatta ccgccaggcc gtggtgaccg cgcgcgttgc gtgcgagatc ccgcatcaga
    42661 acgttgccta tgaacggttt acccctgagg gaccgctcgc gctgctgcct agcggcgagc
    42721 gactcgcgct ggtatggacc accacccccg agcatgcaca gcaattgtgc gaactgcccg
    42781 aggctggctt tctcgcacgg ctacaaagcg tattcggagg ccgacttggc gctttcagcc
    42841 gccccagcgc cccctcccgc ttcccgcttg ccctgaaagt ctcgaaccgc attgcgaaac
    42901 cgcgcatggt ggcgctcggc aacgccgcgc aaacgctgca tccggtcgcc ggccagggct
    42961 tgaacctcgg gttgcgggat gcctgggaac tggcggcgac gatcaaagac cgtaacgcag
    43021 acgaaatcgg cggtgccgcg cacctcaagg cctatggggc gctgcggcgc ctcgaccgcg
    43081 gcggaggcat actcttcacc gacgcgctgg tgcggttgtt ctccaacgat atcgtgccgc
    43141 tcaggctcgc ccggggcctg gggcttgcgg cgctcggcgc cgtgccgccg gccaaggact
    43201 tcctgatccg gcgcatgatc ttcggcgcgc gcggctagcc cgttccaact ggcttctcct
    43261 gactcgagcg agccgccagc gcgcgcccat ccaaacctct tgaaccgctc ggattgccac
    43321 cgatagttgg aacggggtga gctgcggcgc ttgcaaccga ttttcattcc tccgcgtcct
    43381 tgcgcatcgg gagttatgcg ccgtcgcgca agcactgctc gaaaattttt taataagtgc
    43441 ttacttcggc aagcggtttg gtgacgcagc gctgatgcgc gagcggaggt gtgggttcgt
    43501 tgcaacaccg atcaaaaaaa aagcaagaat tgcttttctt gcgcgcgcgc agcgattaca
    43561 atgacgccct tcccctgaaa caagacgact tcgatcgcgg acacggcacg cgcgatgcac
    43621 atcggcccgt atcaactcaa gaacaatctc atcgtcgcgc cgatggcggg tgtcaccgac
    43681 cggccgttcc ggcagctgtg caaggccatg ggcgcgggca tggcggtttc cgagatggtg
    43741 gcgagcaatt cgctcctgtg gggctcggag aaaacgcgcc gccgcgccaa tcacgacggc
    43801 gaggtcgagc cggtctcggt gcagctcgcg ggggcggacc ccgggatgct ggcggaggcg
    43861 gcgcgctata acgtcgacca gggcgcccag atcatcgaca tcaacatggg ctgcccggca
    43921 aaaaaggtct gtaacgtcat ggcgggctcc gcgctgctca aggacgagcc gctggtcggg
    43981 cgcattctcg acacggtggt ggcggcggtg gatgtgccgg tcacgctcaa gatccgcacc
    44041 ggctgggacc gcgagaaccg caacgcgctc aaggtcgccc ggactgcgga gcaggccggt
    44101 gtcaaggcgc tcaccatcca cggccgcacc cgcgcgtgcg ggttctcggg cgaggccgag
    44161 tacgacacca tcgcggcggt gaaggccgag atcgggatac cagtggtcgc caacggcgac
    44221 ctctccacgc ccgaaaaggt gaggcaagtg ctggactata ccggggccga cgctgtgatg
    44281 atcgggcgcg ccgcccaggg ccggccgtgg atgttccgcg agatccagca ctttttgcag
    44341 accggggaac ggctcccgcc ccccgaagtg gaagagatcc atcgcgtgct gctcgcccat
    44401 ctcaacgagc tctacgagtt ctacggtgag gacacgggag tgcgcgtcgc gcgcaagcat
    44461 atttcctggt acaccaaggg gttggcggga tcctgcgcgt tccgccacgc ggtgaaccgg
    44521 gtcgagaccc gcgccgagca agtagcggcg gtcaacgagt ttttcgcgca gctcgcggag
    44581 cgcgggcggc gcctgaccta tgtcgaggaa cttgcagcat gatcaacgaa aatgaaatgg
    44641 cgcgcatggt gcgcagggcg atcgagggct atttccgcga cctggacggg gagaagccct
    44701 gcgcggtcta tgacatggtg atccattgcg tggagaagcc gctgctggag agcgtgctgc
    44761 accgcgtgcg cggcaaccag acccacgccg ccgagatgct cggcatcaac cgcaacaccc
    44821 tgcgcaagaa gatgagggtg cacggcatca agttctagga ttgaggattg agggacgagg
    44881 attgagtgcc cggaacagtt gacggacagc cggttttact caatcctcaa tcctagcacc
    44941 tcaatcccgc cgtcatggcc gtcatcaagc aggcgctcgt cagcgtctcg gacaagaccg
    45001 gcatcgtcga attcgcgcgc ggactgtccg gcttcggcgt tgccttgatt tccaccggcg
    45061 gcaccgccag gctcctccag gacgccggcc tcaaggtgac cgaggtcgcc gactataccg
    45121 gcattcccga aatgctcgac gggcgggtga aaaccctgca ccccaaggtg cacgccggta
    45181 tcctcgcccg ccgcgacgct cccgggcaca tggcggcgct cggcaagatg gccattccga
    45241 ccatcgatct cgtggtcgtc aacctctacc ctttcagcgc ggcggtggcg cgtgccgggt
    45301 gcacgctcgc ggaagcgatc gaaaacatcg acatcggcgg cccggccatg gtgagaagcg
    45361 ccgccaagaa ccacgagcat gtggcggttg taaccgaccc agcggattac gagccggtgc
    45421 tgaaggagat ggcggccacc gccggtgcgc tcggtgccga gacgcggttt cgcttggcgc
    45481 agaaggcgtt ttcccacacc gcgcaatacg acggcgcgat cagcaattac ctgacctcgc
    45541 tcgatgccgc aggcgggcgc gcgcgcttcc cgcaacggct gaacctgcag ttcgagcggg
    45601 tccaggcgat gcggtacggg gaaaacccgc accaggaggc ggcgttctat cgcgatttgc
    45661 agccggcgcc gggcagcctc ggggcctatc gccagctgca gggcaaggag ctttcctaca
    45721 acaatgtcgc cgacgccgat gcggcgtggg aatgcgtcaa gagtttcgag gaacccgcct
    45781 gtgtcatcgt caagcacgcc aacccgtgcg gtgtggcggt cgccgacgcc ctggctggcg
    45841 cctaccgcaa ggcgttcgcg accgacccca cgtcggcctt cggcggcatc atcgccttca
    45901 accgcgagct cgacgggccc acggcccagg ccgtcacgca gcagttcgtc gaagtggtga
    45961 tcgcccccgc ggtcgcgccg gaagcggcgc agcggctcgc gggcaaggcc aacatccgcg
    46021 tgctcgaggt gccgcttgcg cccggtgcca acgactacga cttcaagcgc gtcggcggcg
    46081 ggctgctggt gcagatgccc gacagcgcca gcgtcggcgc ggccggtctc aaggttgtca
    46141 cccgggtgaa gccgaccgag agccagatcg cggacctcct gttcgcgtgg cgcgtggcca
    46201 agttcgtgaa gtccaacgcc atcgtgttct gcggcggggg gcaaacgctc ggcgtcggtg
    46261 ccgggcagat gagtcgcgtg gactcggcgc gcatcgcagc catcaaggcg cagagcgccg
    46321 ggctcacgct cacgggatcg gtggtcgcct ccgatgcctt ttttccgttc cgggatggcg
    46381 tggatgccgt gatcggcgca ggcgcccgcg ccatcatcca gccgggcggg agcctgcgcg
    46441 acgaggaagt gatcgccgcc gccgacgagc acggcgtggc gatggtcttc accggctatc
    46501 ggcactttcg gcactgagtt accgaaagtg cggtgaatag tgaatggtga acggtaagcc
    46561 cctgcgatcc ggcgcgttca cggttcgcga atcacgaatc acgaatcacg attcacgcat
    46621 ctcatgaagc tccttgtgat cgggtcaggg gcgcgcgaac atgcgctggc gtggaaactc
    46681 gcgtgctccc ctcgcatctc gcgcgtgttc gtcgcgcccg gcaacgcggg caccgcgcgc
    46741 gaggacgggc tcgccaacgt ggcggcggtc tcgataccgg aactcatcgc gttcgcgaga
    46801 aagcagctgg tggcgttcac cgtcgtcggc ccggaggcgc cgctcgccga gggcatcgtg
    46861 gatgcgttcc aggcggcggg attgaggatt ttcggaccga cccgggcggc cgcccggctc
    46921 gagagctcca aggagttcgc caaacgcttc atgctgcgcc acggcatacc caccgcgcaa
    46981 tatgcggcat tcagcgaacc cgcgtcggcg cacgagcaca tcgagcgcag cggcgcgccc
    47041 atcgtcatca aggccgacgg tctcgccgcc ggcaagggcg tggtggtggc gatgagcgcc
    47101 gccgaggcgc acgcggcggt ggaccggatg ctgaccgaga aacggctcgg cgaggccggt
    47161 gcccggttgg tgatcgagca ctacctcgac ggcgaggaag cgagcttcat cgtgctcgcc
    47221 gacggcaagc atgcgttgcc gctcgcgacc agccaggacc acaagcggct gctcgacggc
    47281 gatcgcggac ccaataccgg cggcatgggc gcgtattcgc cggcgccggt ggtcagcccc
    47341 gccgtccacg ccaggatcat gcgcgaggtg atcctgccgg cgctcgccgg catggaaaag
    47401 gacggcacac cctacagcgg ctttctctac gccggcttga tgatcacacg ggacggccgg
    47461 gtgcgggtgc tggaattcaa ctgccgcatg ggcgaccccg aaacgcagcc gatcctcctc
    47521 cggttgaaga acgatcttct ccccctgatc gagcacgcgc tggccggcac cctcgacaag
    47581 gtggaggccg agtgggacac gcgggtcgcg ctcgcggtgg tgctcgccgc gcacggctat
    47641 ccggaagcgc cgcgcaaggg cgatgcgatc cacggcctgc cggcgtccca ggaggactat
    47701 cacgtgttcc atgccggcac ggcgttgcac ggaaacgagg tggtcaccaa cggcgggcgt
    47761 gtgctcagcg tcacggcgct gggaggcaac ctgcgcgcgg cccagcgacg cgcctacgag
    47821 atcaccgaga gaatccgctt cgagggcatg caattccggc gcgacatcgg ccaccgcgcg
    47881 ctcgccgcag gccgcaagta ggccgggagg gagctcgatc atggcgatgc actacgaccg
    47941 cgcgcgcgag gacccaggca atatcgtcct gctcgagcac gtcgacctca ccatccccga
    48001 ccagcgcctc gccacgatct tctacatctc cggcctgggg ctgacgcgcg acccctgtct
    48061 caccatcgag cacgaggtga gaagccttac tcacccgctc tacgcgcgcc ctcttgtcaa
    48121 ccgcagtccg gagcagacca accgcgatta cgcgcgcggc cacgactcat tgcgcggcgc
    48181 gtattgatcc agcttggggg atgataaggg ggcgtcgctc ccttattcag caccctgcta
    48241 gaatcccccc ggacattccg cgaaacagcg ggtctcgtcg cggaggataa gatcagcgga
    48301 gaggaggcgt tcccatgcca gccccgtttg gtattcgtcg tcggttcctc gcggtcgtgg
    48361 cgctgctcgc ggtcatcccg gcggcgccgg cccagcaggc gttccccggc aagaagccaa
    48421 tcgagttcat cgtgctgttc cccgccggca gctccgccga cgtcaccgcg cgcgtgctgg
    48481 ccgagggcat ggcgaggcaa ctcgagaccc agatcgtggt ggtgaaccgg cccggcggcg
    48541 gcggggcggt gggctacaag tacgccgccg cgcaggcggc cgacggccac cacctggtct
    48601 ggaactcgaa ctcgatctcg accacctacc acaccggcac gctgccgttt gactaccgcg
    48661 cgttcgcgcc ggtggcgcgc gtgcagatcg aaacgccgtt gctgttcgtg cggagcgagg
    48721 cgccgtggaa gagcctgggc gaggttctgc gttacacgaa agccaatccc ggcaggctca
    48781 ccgtcggcaa ctccggctcg ggcagccata cccatttctc ctcggtgtgg ctgttccggg
    48841 gtgccggcgt gcaggtcgtg gacgtgccgt tcaacgcggc gcaggtggtg ccgagccttc
    48901 tcggcgggca cgtggacgct gtggtgcagc tgcccggcgc gctcgccggt cacgtcaagg
    48961 ccggcaccgt gcgcgcgctc gtcgcgctga gcggagcgcg cgatccgcac tttcccgagg
    49021 tgccgactgc ccgcgagcag ggagtgaatg ccgcggccga gctgtggcgc ggcgtcgcgg
    49081 tgcccagagg cacgccgccg cgcatcgtcg ccaggatcga ggatgcaatc cgcaagaccg
    49141 tgtcgggccc cgaattcgcg agagccaccg aaaaactgct ggtcgcgccc gccttcctgc
    49201 cggcgtccga cttcggtaag ctgatcgcca gggaggacac ggagatcgcg caagcggtca
    49261 aggcgctggg gctcaagccg gactcgaagt gacccgttgg acatcgccgc cgtcaagcag
    49321 tttctgatcg ggttgcagca ggcgatcgtc gcgaggcttg ccgaagccga cgggcagggc
    49381 ttccggcgag acgagtggac gcgccccgaa gacggaggcg gcatcacgca gctcatcgag
    49441 gagggcgcgc tattcgagcg cgccggggtg aatttttccc acgtctccgg ccagggcctg
    49501 ccaccctcgg ccagcgccag ccggccggag cttgccggcc gctcgttcca ggcgatgggc
    49561 gtctcgctgg tcctgcatcc acgcaacccg tacgtcccca ctgtccacat gaacgtgcgc
    49621 ttcttcgtgg cgcagaagaa cggcgcgggc ccggtctggt ggttcggcgg cggcatggat
    49681 ctcacccctt actacccctt cgccgaggac gcgcggcatt tccaccgcac ctgccgcgac
    49741 gcactcgcgc cctttgggcc cgaccgctat ccccgctaca agcggtggtg cgacaactat
    49801 ttctacctca agcaccgcgc ggagccgcgc ggcttgggcg gcattttctt cgacgacctg
    49861 aacgagcccg atttcgcgac ctgcttcgcg ctggtgaaga gcgtgggaac ccatttcctg
    49921 ccggcgtacg ttcccatcct cgagcgccgc agggggattg cctacggcag ccgcgagcgg
    49981 gcgtttcagg cctatcgccg cggccgctac gtcgagttca acctggtgta cgaccgcggc
    50041 acgctgttcg ggctgcaatc gggcgggcgc gtcgagtccc tcctcgtgtc gctgccgccg
    50101 cgggtcgaat ggcgctacga ctggaagccc gagccaggca gcccggagga gaagctctac
    50161 accgagtttc tggtgccgcg ggactgggcg gactaatagc agtgcggagt gctgagttct
    50221 gagtgctgag ttttcagtgt tgttcgagcc cgcttgactc gatactcagc actcagcact
    50281 tagcactagc caccgcgtcg cggcggccta gaaccagctc ttcagcgatt tcactcgcgc
    50341 caggatcttc cggctgaccg agccctcgac gcccgccgcg gcgaccttgt gctggtgcgt
    50401 gaggtggggg ttctgcttga acgactggta gcgaatgagc gcggcctggg tgtggctctt
    50461 cagcagctcg ggattcaccg gcttgttgat gaagcggaag atctgcgcct ggttgataag
    50521 ctcgatcacg agctccgagt cggacgcctc ggtcagcacg atgacgagga tctcggggcg
    50581 ctcgtgcttg aggaccttga aggcggcgag cgattcctcg ccccggctcg cgatgtcggc
    50641 gatgatgagc gccgtttcgt gcgcttccag ctggcccagc gccgcgtcga agtccttcgc
    50701 gtggcgcacg ggacaggctc cgcccacgaa gcgcttcacc gcgcggagcg tcgcttcacg
    50761 cgcctcgatc accagcacgc ccgcctccat cttgtcggga acgatgggcg gggtcgccgc
    50821 ggtttccgca agctcgagcg cgatcgccgc cgcttccccg atggtgttct ggatatcctg
    50881 gttgtcccag ggcttggaga cgaagcggaa cacctcgcct tcgttgaccg agccgacgat
    50941 cgccgcgaga tccgagtagc cggtcaggag caaccgcaca gttgccggcg ccgcttcctt
    51001 cacccgcctg agcagctcca cgcccgtcat ctccggcatg cgctggtcgc tcaccacgac
    51061 atggggctgg aatttcctga cgaactcgag cgcctcgggg ccgttggtcg ccgtgaacac
    51121 gtgatagttc atgcggaaga ccgagcgcag cgccgtgaga atgcgctcct cgtcgtccac
    51181 gaacagcagg cgcgccttct tgatcgaggt ctcggtcgcc aggccggcgg gcaccgcggc
    51241 gggaacggcc gccggcgcgg cggccggggt cggcatgctg ggctgggtcg gcatatccac
    51301 cgtcgcctgc ttcaggtcgg cgagcatgcg cgcggcctgg accagcttgg ggtcgatgcg
    51361 ctgggtgggc tcgtccgcga tggcggccgc agcggtctcg cgctggcttt gccggatgct
    51421 cgcctcgatg cccttgacga acgcgtcggc gaactccttc gcggtctgga agcggtcctc
    51481 gcgcttcttg gcgagcgcct tctgcaccgc ccagtcgagc tgcaccgaga tcttggggtt
    51541 gtattcggaa gggttgaccg ggcgttcgtt cagcgcctgg cgcatcacct cggcgttgtt
    51601 gccggtgaac gggcgccgtc cggcgaggag ctcgtaggcg atcacgccca ccgaatagat
    51661 gtcgctgcgc gcgtcgatct cggtgccgag gaactgctcg ggcgacatgt agcccggcga
    51721 gcccatgatc tcgcccacct gggtgagggt ggaggaatcg aggcgcgcga taccgaaatc
    51781 ggtgagcttc aggcgcccgt cgtcattgat caggatgttg gagggcttga tgtcgcggtg
    51841 gacgacgccg agcgagtgcg catagcccag cccgtcgagc agttgacgga cgagctcgcc
    51901 gatctccttc agcccgaact tcgcctggtt ctggatgtgg tggaacagcg aatggccgtt
    51961 caccagttcc atcacgatgt aggccatttc cgtgtcttcg ccgtagtcgt agatcgcggc
    52021 gatgcgcggg tgggtgagcc tccccaccgc ctgcgcctcg tgccggaagc gcgtgagcgc
    52081 gtactggagg tcggccgggt cgagctccga cttgatgatg gtcttgaccg ccaccggccg
    52141 gtggatcgcg ggatcgaacg cgcggtagac gatgcccatg ccgccctggc cgagcacgtc
    52201 ctgaatctgg tacttgccga tggtggacga ggtgttcatg gttcgtcggg gaggcggatc
    52261 gcgctcaagc cgcctgttgc gccggttccg ggggtgtcaa cggtagcacc accgtaaact
    52321 tggtgccaac cccgacggcg gagtccacgc tgatgctgcc gccgtgctgc tggacaatct
    52381 tgtaggcgat cgagagaccg agcccggtgc ccttgcccac gtccttggtg gtgaagaagg
    52441 ggtcgaagat cttgggtagg acctcgggcg ggatgccctt gccgttgtcc tgaacttcca
    52501 ccgccacgtg tccggcaccc tcgcggcgcg tggtgagctg aatcacgccc ttccccgact
    52561 cggtggcttg cgccgcattg ttgatgagat tgaggaacac ctggttgatc tgcgagggcg
    52621 agcaagtgac tgcaggaatg tcgccgaaat ttttcttcac gatatgatgc ttcaattcat
    52681 gcttggcgag cagcagcgtg ctctcgagcc cttcgttcag gttgaagctc gtcaccttgc
    52741 tgcggtcgag ccgcgagaaa ttcttgaggt tcagcaccag ctcggaaatc tggttgatgc
    52801 cgtggagccc gtccttcacc agcgtctgca gctcgtccag cacgcggtgc tccctgaact
    52861 ggccgatcag ctgctcggtc agcgcgaact gctgggcgag ctgatgcggg tcggccgcgc
    52921 cggagcgcag cagctcgagc aacttctcgg attccgcggc cagccggacg agctcaggca
    52981 gcttccccgt caccgagccg agactgttct tgacataggc aagcggcgtg ttgatctcgt
    53041 gcgccacgcc cgcgaccatc tgcccgaggg aggacatttt ctcggtctgg atgagctgcg
    53101 cctccgattc cttcaattgc cggagcgctt cggtgagttc ctgggtgcgc tcggcaacgc
    53161 gcagctccag cccctcgttc acgctcttca gctcggcgtt ggcggcgttg agcaagcggt
    53221 agctcgcgat caggcgcgtc cccagatagc ccaacaggat cagcagcgcg cccgagtaag
    53281 cgacgaggta ggcgcggtag aactcctggt cggcgagcac catctcgagc tcgcgcgaga
    53341 acgcgttggt caggctgtcc acgcgcggtc ccgcggtgag gaacgagagc ttcacgaaca
    53401 gcgcctcctc gaccggcctc gcgccgagca gctgctgcac gtggtcgtcg agacgatgga
    53461 tgccgtcgcg cagcgccggg ggcagatggc ccgccgcctg gcgcaggtcg gagatgaccg
    53521 tttcgacaag cttgcgctgc gcctcgcttg gggtgaagta atagcgctgc gtctcggcga
    53581 ccgcctgcac cgccgcgctt tccgccgcca ccaggcgctc ccgctcgcgg aagtcctgcc
    53641 acgaaccccg caccagcccc gcgatctcgg cctcggccgc cagcaccctc gcgagcatct
    53701 gcttggtggc tgcgttcgcc ttccggaact tgtccaccat cccggccttc tgcgagaaag
    53761 cgctggcgag atccgggagg ccgcgcgcca gtaccggact gccgagcgct tgcgccgctg
    53821 cgccgagctc tttgccgagg cgggcgagcg ccggtccgta gtcgggggac ggcgtctgcg
    53881 gcggggcaaa ctcggtgcgc aaacgcagta tgtccacgtc ccagcgcgcg tcgatttcct
    53941 tcagttcccg cagcgccgcc agcacctcgt tgctgcgctt gatgtcggcg ccctgggtct
    54001 tgacgtagag atagccgagg atcgcggcga gcagaatggc gccggcggcg atgagcaccc
    54061 gacgggcggt cttcaccgct aggatgcgct gcaatcccgc taagagggtc gtcggttccg
    54121 tcatcgcccg ctcattcgcc gagataggcc tgccgcacct ggggattcac gagcaggcgc
    54181 cgcgcgctgt cggcggccgc gatcagcccg ctctccatga cgtagccgcg atggcacacc
    54241 tcgagcgcga gctttgcgtt ctgctccacc agcagcagcg tcgcgccctc ggcggcgatt
    54301 gcgcgtatgg tttcgaagat cttttgcacc atcagcggcg cgagccccat actgggttcg
    54361 tcgagcagca gcagccgcgg ccggctcatg agcgcgcgcc cgatcgccag catctgctgc
    54421 tcgccccccg aaagcgttcc ggcgagctgc cggcgccgct cggcgagccg cggcaggagc
    54481 ccgtatacgc ggtcgaaatc ggcagtgatg cgcgccgaat cgcgtcggct gtaggcgccc
    54541 atcgcgaggt tttcctccac ggtgagcagc ccgaacacgc cgcgcccctc gggcaccagc
    54601 gccagtcctc tccggaccag ctcgaaagcc gggcgcgctg ttatgtcgtc gccattatag
    54661 tggatgcgac cggccgcggg ccggagcagc ccggcgagcg ccttcagcgt cgtcgtcttg
    54721 ccggcgccgt tggcgccgat cagcgtgacg agctcgcccg ctttcacctc gagatcgatg
    54781 cccttgacgg cgttgatgcc gccgtaggag accttgagcc ccctcacctc aagcaagctc
    54841 gcctcccagg taggccgcga tgaccgcggg atcgcgccgc acctcctgcg gcgcgccctc
    54901 ggcgatcttg cgtccgtagt cgagcaccgc cacgcggtcg cacagaccca tcacgagctt
    54961 cacgtcgtgc tcgatcagga gcaacgtcat gccgtcgccg cggattctcg cgagcagccg
    55021 cttgagctcc tcccgctcgg tcgcgttcat gcccgcagcg ggctcgtcga gcgcgagcag
    55081 cttcggttcg gtcgccagcg cccgcgcgat ctcgaggcgg cgctggtcgc cgtaggagag
    55141 gctgtaggcg ggctccgccg cgcggccggc gatgccgacg taatcgagca gggcgtaagc
    55201 ccggcccgcg accgccgctt cctcggcgcg ggtcgccgcg tcccgcaaca ccgcgccgaa
    55261 cacgccggca cgggtgcgtg cgtggcgccc gaccatcacg ttctcgagcg cgctcatgtt
    55321 cgcgaacagg cggatgttct ggaaggtgcg ggcgatgccg cactccacga cccggtgtgg
    55381 cttcgttccg gtcaagctca cgccgtcgaa ggcgaactcg ccgctgtccg gccggtagag
    55441 cccggtgagc acgttgaaaa gcgtggtctt gcccgccccg ttgggcccga tcagcccgaa
    55501 gatctcgccg cgctcgaccg tgagcgaaac ctccgcgagc gcggcaagtc ccccgaagcg
    55561 cttgctgatc gcgctcgcgg aaagcagcgc gccgctctca gcgtgccgcg tcataaacgc
    55621 ttgcctgttc ctggcgcgca atggcgccct cggcggcaag ctcgcggcgg cgcaccggcg
    55681 agggccacag cccggaaggt cgcagcagca tcaccaggat cagcgccagg ccgaacagga
    55741 gcagccgcag atcggcgggg tcggcgagca cgcgtccgaa caaggcctgc tgcagcggcc
    55801 ccagatagcg caacgcctcg ggcagcacca ccagcagcac gacgccgagt atcacgccgg
    55861 ggatgtggcc catgccgccc agcaccacca tgcacaggat catgacggat tcgatcaacg
    55921 tgaagctctc ggggctgacg aagccctgga agccggcgaa cagccccccg gataagccgc
    55981 cgaagcttgc gcccatggcg aacgcgagca gcttcacgtt gcgggtgttg atgccggtgg
    56041 ccgttgccgc cacctcgtcc tcacggatcg ccacccacgc gcgcccgatg cgcgaatcct
    56101 cgagccgcat cgagacgaag accacggcga gcgtcaacgc gaggaacaga tagtagtagt
    56161 tgtagacggt gggaaaagtg agcccgaaaa aggagtgcgt ctgggcagcc gaccactcgc
    56221 ccagccggat ggggtcgatc aggttcacgc cctgcgggcc gttggtgatg ttgaccggcg
    56281 cgttgagatt caccaggaag atgcggatga tctcgccgaa gccgagcgtc acgatcgcga
    56341 gataatcgcc gcgcagccgc agcgtaggcg cgccgagcag cacgccgaag acgcaggcga
    56401 acaccgcgca cccggggagc aggacccaga acggcaggtg gaggttgaaa tggggggatg
    56461 cgagcagagc gtagagataa gcgcccaccg cgaagaacgc cacgtaaccg agatcgagca
    56521 ggccggcgta tccgaccacg atgttcaagc ccagcgcaag catgatgtag agcagtgcga
    56581 aagcggcgat acgcacccag gtgcggccga ccgcccagtc ggtgacgaac ggcagcgcga
    56641 tgagagcggc cgcgagcagg agataggcga cccacacgaa cctggggtcg cgccgccacc
    56701 tttgccatct ttgccctagt tcaagcatcg cttctctttt gcgatgtaag catcaagcgg
    56761 taagcggtga gcagtaaagg gcttgcatac tgctttttcc gcatgccgct gacagctcac
    56821 tgcttacgat ctttcggcta cgcgctcgcc gagcaggccc gagggtcgga acaccagcac
    56881 caggatcagc acaaaaaaag cgaacacgtc ctggtaatgg ctgccgagga atccgccggt
    56941 gagcgctccg atgtaaccgg cgccgaggct ctcgatcacg cccagggcca cgccgcccag
    57001 catggcgccg gcgaggttgc cgatgccgcc cagcaccgcg gcggtgaacg ctttcaggcc
    57061 cagcatgaag cccatggagt agtgcgcaat gccgtaatag gcgccgacca tcagcccggc
    57121 caccgctgcg agcgcggagc cgatcacgaa ggtgatcgag atcacagtgt tgatgttgac
    57181 gcccatcagg ccggcgacct cgggattctg cgaggtggcg cgcatggcgc gtccgagccg
    57241 ggtgcgatgc acgagccaga gcaatcccgc catgatggcg aaggcgagcg cgacgatgaa
    57301 gacctggagg tcggtgacgg tcgcaccggc caacgcatgg ggggtggacg gcagcagcgc
    57361 ggggaacgag tggtactgac gtccccagat gatcatcgcc acgttctgca gcaggatgga
    57421 catgccgatc gcggtgatga gggcggcgag ccgcggcgcc tgccgcagcg ggcggtaggc
    57481 gacgcgctcg atggcgtaac ccagcgccat gcacgccgga atggcggcgg ccaatcccag
    57541 cgccgcgatg aggggccccg gcagcgcgac cccgcttgcc atcagcaact ggatcacggt
    57601 gagcgccacc atcgcgccga tcatcaccac ctcgccgtgc gcgaaattga tcagccccag
    57661 gatgccgtaa accatggtgt agccgagcgc gaccagcgcg tagatcgcgc ccagcacgat
    57721 gccgttgatg agttgctgca ggaagatgtc catgcgccgc ttaggcgttt gtcggactta
    57781 ttagacccga caaacgcctg atgcaaaacc acccgcaaga aaatggctgg aaagggctcg
    57841 agcgacgtga attcgacccc gctcccgtca ccaacggcgg gcgtgaggag ggtatttcat
    57901 tcaacttcat cctgaaccct cgggcggttt tgctgttagc agcctgtcgc accagcaagt
    57961 ccgacaggct gctaaatgca aaaacggcac ccgaaggtgc cgtctacaca ctgatgacgg
    58021 acgtccatca cgtcatcgtc attttttctc cgcggccggc gcgggtgccg cggccgcccc
    58081 cccgctcacc gtctcgagcg gatcccactt gctctccttc acgacgtaga gcgtgatcgg
    58141 accattcctg agatcgccct tttcatcgaa cgcgatcggt ccggtcacgc cctggcgctg
    58201 gatcttcgcg agctccggca ggtacttcgc cggctcggtg gagttggctt tcaccatcgc
    58261 ctcgaccagc gtcatcgtcg catcgtaggc gtacggcgcg taaatctgga tttgtccgta
    58321 cttggcgttg aatctctcgg tgaaacccgt gccgcccggc attttgtcga gcggcagccc
    58381 cggcgaggag gcgaccacgc cttccgccgc cgcacccgcg agcttgatga actcggggct
    58441 ttgcgcgccg tcaccggcga ggaacctggc cttgaccgcg agcgctttca tctgcgccac
    58501 catcggcccc gcctgcgcgt ccatgccgcc gtagaacacg agatccggct gcctggcctt
    58561 gacgttggtg aggatcgagc ggaattccgt cgccttgtcg ctggtgtatt cgcgcccgac
    58621 gacggtcgcg ccggccgcct tggccgcttt ctcgaactcg tccgcgagcc cttggccgta
    58681 ggcggtgcgg tcgtcgatga tggcgattct cttcgcgccg agcttcgcca ccgcgtactc
    58741 gcccagcacc ttgccctgct ggatgtcgtt tgccatcacc cgaaacgcgt tcttgtagcc
    58801 ctgctgggtg tacttcggat tggtcgcgga gggcgagacc tgcacgatgc cggcgtcgtg
    58861 gtagatcttc gaagccggga tcgtggtgcc ggagttgagg tgcccgatca cgccggcgac
    58921 cttggcgtcc accagcttct gcgcgacgat ggtgccggtc ttgggatcgg cctggtcgtc
    58981 ctcgctgacc agctcgaact tcaccttcac gccgccgatc atgagaccct tcgcattcaa
    59041 ttcctcgatg gcgagccgtg cgccgttgtc gttgtccttg ccgagatggg cctgcggacc
    59101 ggtgagcggc gaaacctggc cgatcttgat cacgatctcg ggtggcgcct tcggcgctcc
    59161 agctttgggc ggcggagctt cttggccgca actggaaagg atcaacgcga acgcgaggcc
    59221 ccagccgatg ccgaagacgg attggcgaac catgacggta tccttaagat aactcgttgt
    59281 tgctgctcgg gaaatcatta tcctaatcgg ccgcgtcctt gtcaatttcg agcagtcacc
    59341 ggccgtacaa aaaatgagcc ggcctggagg ccggctcatt tcacccgtcg cagccgatta
    59401 cttgagggac aggatccact gcaccatcgt cttgatgtcc tcgtccttca cgtgcgcgtt
    59461 gggggacatc ggtatgtcgc cccacacgcc cttgccgccg gccttcactt tcttgatcag
    59521 ctcggcctcg gcgcctttgt tgccgcggta cttcgcggcc acgtccttat aagacgggcc
    59581 gatcagcttc ttgtccacgg tgtggcaggc caggcagccg ctcgtcttgg cgagggcctc
    59641 ggcggtcttg gggtcggcgg cgtgcgcgcc ggcggcgatc gttgcagcgg ccgcagccgc
    59701 gagaagcgtc aggatgtttt tcatcggatg gctcccaggt cagtcaaaaa acccgcgcat
    59761 tgtatcagta gtttgccgga tgtttgtccg gccaggcccc ttatctggaa catggaacag
    59821 cctcaagttc acaggcgctc tcaggtctag ccatggccgg tggacaagat gcgcttaagc
    59881 ccggcgagat ccgtcacagc cggcagacaa gtaacgccgc ggcacaccca ggcgttgacg
    59941 ccggcgggcg gggcgggctt gtccaggacc ggcggcagtc ccgccaggcc ggcgggcagc
    60001 gcgagaacga gtgtgtcggg ccggtaagtc cgcgcgagcg cgtgctgcca ttccgccagc
    60061 gcgcgagcct cgccgcggag gatagcgatc tgcggcggtg cgagatattc gtcgagcacg
    60121 gtggcgaagc tcacgaaacc gctcggctgg cgttccatgg ccgggtagaa gagcttgagc
    60181 gcccgttccg ccgccccgag gtagcgcggc tcgccgatga gatgccccag ccgctgcagg
    60241 gcgagcgccg cgatgccgtt gccggagggg gtcgcgttgt cgtggccggg cttggcgcgg
    60301 tggatcagct tctcgtggtc gtgggagacg aagaaaaagc cgcccgtctc tcgatcctcg
    60361 aaccgttcga gcaatatctc ggccagctcc cgggcgaacc ggagatcgtc ctcgcggaat
    60421 tcgccttgca tgagctcgag cagcgcatcg agcaggaagg cgtggtcatc gaggtaagcg
    60481 ttcaagtgcg catgcccgtc cttgtaggtg gcggtgagcc tgccgtcgcg ccacagtgtc
    60541 gcccggatga actccagcgc ccgccgcgcg gagccgagcc aggccccttc gccgaacgcg
    60601 cgcgcggcgc gcgccagccc ccccaccatc atcgcattcc agctcgtgag caccttctcg
    60661 tcgcgcccgg gcctgacgcg ctgctcgcgt gccgccagca gcttcatgcg cgcgcgcgcg
    60721 agccgcgcct cgcattccgc gagcggtatg gaaagccgct tcgcgatgaa ttcgagcggc
    60781 ttcatcatgc gcagatgcca gtgcccctcg aaattcggcg cgccgtcgag cccgtaatgg
    60841 ggctcgatga ccgcgtactc ttcggccgtg agcaggcttt tcaactcctc cggagtccag
    60901 acgtagtatt tgccctcttc gtgctcggaa tcggcatcga gcgaggagta atagcccccc
    60961 tccggcgatt gcatctcgcg cattacccat tgcgcgatcc ccctcaccgt tttctcgaac
    61021 agcggctgtc gcgtgacgag ccacgcgtcg ctgtagagcg cgacgagcgc cgcgttgtcg
    61081 tagagcatct tctcgaaatg cgggatgctc cagtactcgt ccacgctgta gcggcagaac
    61141 ccgccgccga gctggtcgta gattccgccc tcggccatct tggcgagcgt gagccgcgcg
    61201 atcgaaaggc ccagctcgcc gccgtcgagg gcgtggcgcc gcatgcagaa cgcaagctcg
    61261 aaggggtgcg gaaacttggg cgcctgcccg atgccgccgt gcaggtcgtc gaacacctgc
    61321 gcgagctcgc gcaccacggc gtccagcggc gcgcgcttga ggctgcgccc gctacccggc
    61381 gccggcagcg tacgggaaag cgcttcgagc agcgcggcgt tctgtcgttc gatgtcctcg
    61441 cgcttgtcgc ggtaggcgtc ggcgatccgc ggcaggatgt cgagaaagcc cgccatgccg
    61501 tggcgcgcgc gcttgggaaa gtaggtgcca ccgaagaacg gcgtgccgtc cggcatgagg
    61561 aacatggtga gcggccagcc gccgcttctt tgcgtgagca tcgcgtgcgc cgtctggtag
    61621 atctggtcga ggtcagggcg ttcttcgcgg tccaccttga tgttgacgaa gtgccggttc
    61681 atctccgcgg ccacttccgg gtcctcgaac gattcgtgcg ccatcacgtg gcaccagtgg
    61741 caggcggagt agccgatcga gagcaggatc ggcttgccgg cctcgcgcgc agccgcgagc
    61801 gcctcctcgc cccacggata ccagtccacg ggattatccg cgtgctgccg gaggtagggg
    61861 gaggtctgcg ttgcgaggcg gttgggcatg ggagtgggaa cacgcaagac gggcaaagta
    61921 attttggcac aaagcgcgcc ctcgggttcc cgggtacaaa gtcccaagtt caaaataaaa
    61981 accctcggat cccaatccgc tccaaccttg aacctggaac gcgcgccggg agccgaggtt
    62041 caacgcgcgt cggtaatggc cttgagcgtg gcttcgtact gcgcaagtcg cgggccaccg
    62101 atggcgaccg cgcgctgcgc ggcggcaagc gcctcgtccc ggcgttcgag ctcgagcagc
    62161 gtctgtgcga ggttattcca tgcgtcggcc gcttccgggt ggcggcgggt cgcttcgcgg
    62221 taggccgctt ccgccgccgg gaggtcgcgc atcgcgtagg cggtgttgcc ggagccgatg
    62281 cgcgcgaaaa ggttgtgcgg ccagcgttcc agcgcgctcg catacgcgcg cccggcggcg
    62341 gccggagaaa cccgctccag ggcagcgctt gccgcgacgt agcttccctc ggcggcgctg
    62401 accggtagcc ggtccggcgg catcgtcacc atcgcccagt gctcgccgcg cgcccaggta
    62461 cgctcgaaat tgctgagcgt cgtgacgagc ctgcgggtcg tgccggagcg caacatgatt
    62521 tcctcgcgcg gcaggtcgta gccgaccacc accgcgtaat gccacagggg cgcaaaggcg
    62581 aaggacaggt tctggaacac gactacgggc gtgccggcgg ccacctcctg cagcacgtgc
    62641 tccagccgcg gtgccaggcg gtacgcgacc aggccgtggc ggcgcgccgc cgccagcatt
    62701 tccgcctgca acgatccttc gcgcccgggc aggtaaacct gcggcacgag cgcttcgggc
    62761 gaggtggcga cgccggcgtg caccagcgtc gtcgcaagcg cggccggccc gcactgatag
    62821 agttcctgcg gatagaacgg cacggcatcg agttcaacgc gccccggcag atctgaaggc
    62881 ggccgcgcca gcagcgccgc cacttgcggc gtcgcgcatc ccgcgaggag ccacgcggtc
    62941 aaaagcaaaa cccccgccag ggcgggggtt ttggagcaga acgacacgag gcctagcgcg
    63001 atgaccgcgt gaacggaaac accttggtga gcccgagcag gtcggtgacg agcaggatga
    63061 tgaagatcac cagcaggacg cccagaacgt cagagccgcc ggccggcagc tggtcaatgc
    63121 gggcggccag atcgcgcgct tcctcgtcgg tcagcgcgtc gacacgcgcg cgcgttgcac
    63181 cggcatcgac gccgagcgcc tgcatctggg cgcgcacgtc ggcgcggtca aggaaattct
    63241 tgacttgatc gcgctcggcg ccggccacga tctgatccgt ggtgacgatg cctgccgatg
    63301 ccgggagcgg cattcccatg ccgagggtac agacgatgag cacacgagcg acacagcggt
    63361 tcaagatttt catcgttatt tctcctttga gttggtcagg cctggccacg ggtaaacccc
    63421 ggagagcggg tggcaggcgc cgaccagcat agccaacgcg tgtgtttaac gtcaaacgat
    63481 tactgcgcct cagcgtgaat tattcctcag attcatcagg ttaaggcttt tccgccggga
    63541 tggcctcgag cctggaaggc tgtcgtgcga ggtttccagg ccgatcagcg cgccgttgag
    63601 gacaaagggc ccgatcatcg ccggctcgaa ccagcgcgct tcgacgacgc gctgcgcgaa
    63661 cgccttcatc ggagaccgcc ctgtgtcagt ccgtcactgc cccgcgggat gcgctggaga
    63721 cctgggcggc atacttggcc aggacgccgc gcgtgtagcg cggccgcggc gagcgccacg
    63781 cggcgcggcg gcgcgccagc tcctcctcgg aaacgttgat ttgcagcagc agccgctccg
    63841 cgtcgatcgt gatcgaatcg ccttcgtgca ccagcgcgat cgggccgccg actgccgtct
    63901 cgggcgcaac gtgtcctacg actattcccc aggttccgcc ggagaaacgg ccatcggtga
    63961 tcaatccgac cgactcaccc agcccggcgc cgatgatggc cgaggtaggc gccagcattt
    64021 cacgcatgcc gggaccgccc tttggacctt cgttgcggat cacgagcaca tcacccgcct
    64081 tgacctttcc gccgagtatc gccgtcaagc agtcctcttc cgattcgaaa atacgcgccg
    64141 gcccggtgat ggatttcttc ttgagcccgg taatcttggc gaccgcgcct tccggcgcga
    64201 ggttgccttt gaggatggcg agatggccgt gcgggtacag cgggttctcc cactgccgga
    64261 tcacgtcctg attcaggggc gggcgcgaag gcactttcct caagaggtcg ccgacggtct
    64321 tgccgctgat cgtgaggcag tcgtcgtgga gcagcccgtg agcgagcagc atcttcatca
    64381 cctgcggaat gccgccgacc tcgtgcagat tggtaatgac gtagcggcct gacggcttga
    64441 gttcgcataa caccggtaca cgatgccgga tcgtttcgaa gtcgtcgatc gtcagcggca
    64501 cctccgcggc atgcgccatc gcaagcaggt gcagcacggc gttggtcgaa ccgcccaccg
    64561 ccatcaccac cgagatcgcg ttctcgaagc tcttacgcgt catgatggcg cgcggcaaca
    64621 gctccttcct gatggcgcgg atcaatacct cggcagactc ggcggcgctg tcatgcttct
    64681 ccttgtcctc ggccgccatg gtcgaggaac ccggcaagct catgcccagc gcctcgatcg
    64741 ccgatgacat ggtgttggcg gtgtacattc cgccgcagga gccggcgccg ggacaggcgc
    64801 ggcgctccac cccggtgagt tcctccttgc cgatcttgcc ggcggtgtac gcgcccaccg
    64861 cctcgaacga gctcaccacg gtcagatcgc ggccctggta atggccgggc ttgatggtgc
    64921 cgccgtagac gaagatcgag gggatgttca tgcggccgat ggcgatcagc gcgcccggca
    64981 tgttcttgtc gcagccgccg atcgccagca cgccgtccat gcgctgcgcc tgcaccgcgg
    65041 tctcgatcga gtccgcgatc acttcgcgcg agaccagcga atacttcatg ccctcggtgc
    65101 ccatcgagat gccgtcggag accgtgatgg tgccgaaagt ctgcggcatc gcgccggcct
    65161 tcttcgcggc ggcctcggcg cgccgggtga ggtcgtccag ccccaggttg cagggcgtga
    65221 tcgtggaata gccgttggcg atgccgatga tcggcttcct gaagtcggcg tccttgaagc
    65281 cgaccgcgcg cagcatggcg cggttgggcg agcgctgcac gccttgcgtg atgagctggg
    65341 agcgtttgtt gagtggcatg gaaatctccg gggtggccgt tacgtacgta taatgaggcc
    65401 gccattttaa cgcatccctc acactttccc ggtgctggtt cacccgcaat tcgatcccgt
    65461 cgcgctgcac attggcccgc ttgcggtgcg gtggtacggc ttgatgtatc tggtcgggtt
    65521 cgcgctgctc tgggccgccg gccgctaccg catcgcgcgc tcgtccgggt ccgtgtggac
    65581 ggccagggat ctcgacgacg cgctgttcta cggaatactg ggcaccattc tcggcggacg
    65641 cctgggctac gtgctcttct acaagttctc cgactatctc gccgagccgt ggcgcatcct
    65701 ctacgtctgg gaaggcggca tgtcgtttca cggcggcatg ctcggcgtga tcctcgccat
    65761 ggcgtggttc gcgcggagcc ggcggcagga ctggctcgcg gtcaccgact tcctcgcgcc
    65821 actcgtgccg ctggggctcg ccgccgggcg cctcggcaat ttcatcaacg cggaattgtg
    65881 gggccgcccc gcctcggtgc cctgggcgat ggtgttcccc aacgtggacg gcgtgccgcg
    65941 ccacccctcg cagctctacg agttcgcgct cgaaggcgtt gtcttgttcg cggccctgtg
    66001 gtggttctcg gcgcggcgtc gcccgcgcgg cgcggtctcg ggggcgttcc tgctgggcta
    66061 cggcctgctg cgcttcctcg tcgagttcac gcgcgagccc gaccgcttcc tcggttatct
    66121 cgcgctcggt ttctcgatgg gacagtggct gtcggcaccg atgatcctcg ccggcatcgc
    66181 attgatcgcc tgggcctacc gtcccgccgc gcgttgagcg gcggttgatc cgtccgctcg
    66241 aacgcgcgga gttaaaccgg cgccagcgtc cggctttaac tctaattcgg tttcttgaga
    66301 ccccctgcgg tcgcgctggc cggtttcgcg agcgcggcgc ggcgatcgag cagccgcccg
    66361 aaattcgcga gcgagatcaa gtgcgcgtag acgcgtgcca gtatccccct ctgcgtgagt
    66421 tccttcagct tctcgggcgc gagttccgtg agcctcccct ctgccacgcg atacatgccg
    66481 gtcatggcga gcggcgtgcc ctgattgggg attgcctgca tgatgaaggt ttccaggagc
    66541 ccgagccggg cgagctcgct ggacatctcc tcgcagcgca ccaggtcggc ctcatactcg
    66601 aagagcagct tgcggcgctc ctcccatgtc ggcagtggct cgcctttggc gtcatggagc
    66661 gcctcaccct tgttgttgat cgcgcgcttc tccacgcacg cgacgcgttc cggtcgctcg
    66721 cgtccatcca ctgtcacccg cgtcatgcag aagggatagc gccgcacgta ggcgggaagg
    66781 taggtggcgg ggtcccagcc gccgttcggc gtcacgaaca ggttctgctg gttttccagc
    66841 cccagcaccg ccatcgcgac gaagtggccg ccttcgccgc caatgaaggc gatcggataa
    66901 tcgcggcaag cgacggcgaa ctcggtgaac gagatcggca gcgcggcgag gctatggaac
    66961 acggtcgcca gctttcccgc ttcaggcagc agcacgcggt gctgccgggt aaggggcacg
    67021 atttcctggt aaccataggg cggcttgatt tccatggcgg cttcacgcgt cgggctcggc
    67081 aaattatagc tggatgtccg ctgcgcggcc ccgccaagat tgtcgtttcc cgtcagaccg
    67141 ttgtagaatg ccgccctcgc aggcacggcg tggggacgta gctcagctgg gagagcgtcg
    67201 cgttcgcaat gcgaaggtcg ggagttcgat cctcctcgtc tccaccaaaa aaggccggga
    67261 atcgccctgg tcttttttgt gagcgcgtga gatttccagg aaccgggtcg ggcgttctgg
    67321 cacaataagg ccgcgcatat cctcctggca tcgcgtgacg atcggagcga cgcaccacca
    67381 tgttcggcaa actgctgccc cgcgagggca ggttcttcga gttcttcaac gagcacgctg
    67441 aactgatagt acagggcagc cgcgagctcg ccgcgctgat ggaaggcggc agcgatctcg
    67501 cgcttcgcgc ccacaacatc gaaaccatcg aaaagcgcgc cgacaggatc acccgcagca
    67561 ccatcgagct gctgcacaag accttcatca ccccgctcga ccgcgatgac atccacaagt
    67621 tgatcacgaa gatggacgac atcctcgacc tgatcgagga cgcggcgcag ctggtggtta
    67681 tttacgatat tcgggtcgtc accgccgagg aaaaaaggct cgccgatatc tgcgtgacct
    67741 gcgccgacgg agttaagtcc gcggtcggaa tgctgtcgaa catggagaat gccgccgcca
    67801 tgctggccgc gtgcgaggac atcgaccggc tcgagtcgga ggccgatcat gtcatgcgcg
    67861 cggcgatggc caagctcttt cgcgacgagc ctgacgtgcg cgaactgatc aa
//
LOCUS       DBNGWZ010000086         3803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_133574, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000086 DBNGWZ010000000
VERSION     DBNGWZ010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_133574"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16CX_02655"
     CDS             <1..336
                     /locus_tag="AC16CX_02655"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3011442.1"
                     /translation="ADANQHGDIFGGWIMAQVDIAGSIPAVRLAKGRVATVAVNSFLF
                     KQPVFVGDLVSLYAEVVRVGRTSITVNVEVYAQRKPEREEVVKVTEATLTYVAVDENR
                     RPRVVSAQQ"
     gene            448..1701
                     /locus_tag="AC16CX_02660"
     CDS             448..1701
                     /locus_tag="AC16CX_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR3011443.1"
                     /translation="MKTSRTLAAFAVAMALGSLASGTIAGGFAIGTQSGSGTGNAFAG
                     GAAAADDASVAWYNPAAMTLLPAGKQVAGALHALRPSFKYRDQGSTGAFLASTANGGD
                     GGDWAFVPNAFFTTDINPKWRFGLALNAPFGLKTEYDNGWRGALTALKTEVKTVNVNP
                     AVAFKASDNVSIGAGVSVQKLDATLTNCANVACTNIAELNADDTGYGFNLGLMVQASP
                     STRFGASYRSSIKYGLEGTITFSALPAGNGPIRADLKVPDSASISVFHALNPKWELMG
                     DITWTGWSSVQQLVVTRTTTGAVLTTLPFQWKDTWRFAVGANYKVNDQTKLRFGLALD
                     ETPTNDATRTPRLPDQERTWVAFGMQYRVSKAGVLDWGYAHEFIKDARVNVPVPGFTT
                     CAAGCLSGSFENKADIFSIQYSHSF"
     gene            1801..>3803
                     /locus_tag="AC16CX_02665"
     CDS             1801..>3803
                     /locus_tag="AC16CX_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR3011444.1"
                     /translation="MSSSSPFIVRKAAVLGAGVMGAQIAAHLVNANVSAVLYELPAKE
                     GDPNGNVLKAVENLRKLEPSPLSIASKAGLIQPANYNQHLEQLKECDIVIEAIAERMD
                     WKADLYRKVAPHIGDHAIFASNTSGLSINKLSESFPEHLRKRFCGVHFFNPPRYMHLV
                     ELIPAATTDAAILDDLERFLVTTLGKGVIRAKDTPNFIANRVGVFSMLATVHHAQQLG
                     IGFDVVDALTGTLIGRPRSATFRTADVVGLDTFAHVVNTMKETLPDDPWHRYYEVPAW
                     LKKLIDQGALGQKTRKGVYQKIGSDIHVLDLKTQNYRLSDGKADEGVVEILKNKDAPA
                     RFGELHASSHPQAQFLWSIYRDAFHYCAAHLAEIADSARDLDLAIRWGFGWNQGPFEI
                     WQAAGWHQLARWISEDIAAGKAMAKTPLPAWAMEPGRTGVHGAQGSFAPAASAYKPRS
                     SLPVYRRQPFPDRLLGEEARYGETIFETDGVRCWHTGDDIAIVSFKSRMHAIGDDVLD
                     GVGQALEQAERNYMGLVIWQTEPPFSVGANLKKTPAGGAKPSQPSALGRLFRQVRREA
                     ESLALKAARQIGVADQLMAGKLAEVENLVEQFQDMTQALKFSMVPTVAAVDGLALGGG
                     CEFIMHCDRAVATLESYIGLVEAGVGLLPAGGGCKEFALRAAA"
BASE COUNT          735 a         1177 c         1215 g          676 t
ORIGIN      
        1 gccgatgcca accagcacgg cgacatcttc ggcggctgga tcatggcgca ggtggacatc
       61 gcgggcagca ttccagcggt gcgcctcgcg aagggcaggg tcgccacggt ggcggtgaac
      121 tcgtttcttt tcaagcagcc ggtgttcgtc ggtgatctgg tcagcctgta tgccgaggtc
      181 gtgcgcgtgg gtcggacttc catcacggtg aacgtggaag tttatgcgca gcgcaaaccg
      241 gaacgcgaag aggtggtcaa ggtgacggag gcgacgctga cctatgtcgc ggttgacgag
      301 aaccggcgcc cgcgtgttgt atcggcgcaa caatagccgg ccgcctccgc tcgaatatgt
      361 tgtatcgtga tcggccagag gtaatatagc ctgcacattt cctgccgcag gaacgaagcg
      421 gccggctatt caaagagggg agacacgatg aaaacatcca gaacactggc cgcgtttgcg
      481 gttgccatgg cgttgggcag cttggcgtca ggcactattg caggcgggtt tgcgatcggc
      541 acgcaaagcg gcagcggcac gggcaatgca ttcgcaggtg gcgcggcggc ggccgacgat
      601 gcgagcgttg cctggtacaa ccctgcagcg atgaccttgc tgcccgccgg caagcaggtg
      661 gccggcgcgc ttcacgcatt gaggccatcg ttcaagtacc gggaccaggg ctccaccggt
      721 gctttcctgg cctcgaccgc caacggcggc gacggcggcg actgggcgtt cgttcccaat
      781 gcgttcttca cgacggacat caatccgaaa tggcgtttcg ggctcgccct caacgcccca
      841 ttcggcctga agaccgagta cgacaatggc tggcgcgggg cgcttacggc cctcaagacc
      901 gaggtcaaga ccgtcaacgt caatcctgcg gttgcattca aggcgagcga caacgtgtct
      961 attggcgcgg gagtcagcgt gcagaagctc gatgccacgc tgacaaattg cgcgaacgtc
     1021 gcctgtacta acatcgccga actgaacgcg gacgacacgg gctatggatt caacctcgga
     1081 ttgatggtcc aggccagccc gagcacgcgc ttcggggcgt cttatcgctc ctcgatcaag
     1141 tacggtctcg aaggcacgat aaccttcagc gccctgcctg caggcaacgg cccgatcagg
     1201 gccgatctga aagtaccgga cagcgcttcc atcagcgtct tccatgcgtt gaacccgaaa
     1261 tgggaactga tgggggatat cacgtggacc ggctggagct cggtgcagca actggttgtt
     1321 acccgtacta ccactggcgc tgtattaacc acgctcccgt tccagtggaa agatacctgg
     1381 cgtttcgcgg ttggcgcgaa ctacaaggta aacgaccaga cgaaactgcg ttttggcctc
     1441 gctctcgacg aaacgcccac gaacgacgcc acgcgtacgc cgcgattgcc cgaccaggaa
     1501 cgcacctggg tcgcgttcgg gatgcagtac cgggtgtcga aagccggtgt gctcgattgg
     1561 ggctacgccc atgagttcat caaggatgcg cgcgtcaacg tgcccgttcc cggcttcacg
     1621 acgtgcgccg cgggctgcct gtcgggaagc ttcgaaaaca aggcagatat cttctccatc
     1681 cagtactcgc actcattcta ggcggaaagg ctcggcgaaa aggccaggcg ggatccgcct
     1741 ggccttttta ttttctgctc acggccgcgt gctagaatct ctctccaatc gggagggttc
     1801 atgtcatcct cttctccctt catcgtgcgc aaggccgcgg tgctcggcgc cggcgtgatg
     1861 ggcgcgcaga tcgcagcaca cctcgtcaac gcaaacgtct cggccgtcct ctacgagcta
     1921 cccgccaagg aaggcgaccc caacggcaac gtgctcaagg cggtcgagaa cctcaggaag
     1981 ctcgagccaa gcccgctctc gattgcatcg aaagcgggct tgatacaacc cgcaaattac
     2041 aaccagcatc tcgagcaact gaaggagtgc gacatcgtca tcgaggccat cgcggaacgg
     2101 atggactgga aagccgacct gtaccggaag gtggcacccc atatcggcga tcacgctatt
     2161 tttgccagca atacgtccgg tctttcgatc aataaacttt cagaatcctt tccggagcat
     2221 ctccggaaaa gattctgtgg ggtgcatttc ttcaacccgc cgcgctacat gcacctcgtc
     2281 gagctgatcc ccgcggccac caccgatgcg gcgattctcg acgatctcga gcggtttctc
     2341 gtcaccaccc tgggcaaggg cgtgatccgc gccaaggaca cgcccaactt catcgccaac
     2401 cgcgtcggcg tgttctcgat gctggcgacc gttcatcacg cgcaacagct cggcatcgga
     2461 ttcgacgtgg tggacgccct caccgggacg ctgatcgggc gcccgcgcag cgcgaccttc
     2521 cgcaccgccg acgtggtggg gctcgacacg ttcgcgcatg tcgtcaacac catgaaggag
     2581 acgttgcctg acgatccctg gcaccgttat tacgaggtcc ccgcgtggtt gaagaaattg
     2641 atcgaccagg gcgcgctcgg ccagaagacc cggaaagggg tgtaccagaa gatcgggtca
     2701 gacatccacg tgctcgatct caagacgcag aactaccggt tgtccgacgg caaggccgat
     2761 gagggcgttg tcgaaattct caagaacaag gatgccccgg cgcgcttcgg cgagctgcac
     2821 gcttcgagcc atccgcaggc gcagttcctg tggtccatct accgcgatgc tttccattat
     2881 tgcgccgcgc acctcgccga gatcgccgac agcgcgcgcg acctcgatct cgcgatccgc
     2941 tggggtttcg gctggaacca gggaccgttc gagatctggc aggccgccgg ctggcaccag
     3001 cttgccaggt ggatatcgga ggacatcgcg gcagggaagg cgatggcgaa gacgccgctg
     3061 ccggcctggg cgatggaacc gggacgcacc ggcgtgcatg gcgcccaggg ctcgttcgcg
     3121 ccggcggcca gcgcctacaa gccgcgctca tcgctgccgg tctaccgccg ccagcctttc
     3181 cccgaccgcc tgctggggga ggaagcgaga tacggcgaga ccatcttcga aaccgacggc
     3241 gtgcgctgct ggcacaccgg cgacgatatc gcgatcgtca gcttcaagag ccgcatgcac
     3301 gctatcggcg acgacgtgct cgacggcgtg gggcaggcgc tggaacaggc cgagcgcaac
     3361 tacatgggcc tcgtcatctg gcagaccgag ccgccgttct cggtcggcgc caatctcaag
     3421 aagacgccgg ccggcggcgc gaagccctcg cagccgtcgg cactgggcag gcttttcagg
     3481 caagtcaggc gtgaagcgga gtcgctggcg ctcaaggcgg cgcggcagat cggcgttgct
     3541 gatcagttga tggccggcaa gctcgccgag gtggagaatc tggtcgagca gttccaggac
     3601 atgacgcaag ccttgaagtt ctcgatggtg ccgaccgtcg cggccgtgga tggccttgcg
     3661 ctgggcggag gatgcgaatt catcatgcat tgcgaccggg cggtggcgac gctcgaaagc
     3721 tatatcggcc tggtcgaggc cggcgtcggg ctgctgccgg cgggaggcgg ttgcaaggag
     3781 ttcgcgctgc gcgcggcggc gga
//
LOCUS       DBNGWZ010000087         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3030429, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000087 DBNGWZ010000000
VERSION     DBNGWZ010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3030429"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1599
                     /locus_tag="AC16CX_02670"
     CDS             <1..>1599
                     /locus_tag="AC16CX_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3011445.1"
                     /translation="AGFMADVYYRVSGKPTATFTSCGPGSANLPIALANSFFDSVPFL
                     AVTGNVPTSQFNCGAFQETYRHYQADYPSTVRAYCKRVFQPTTAAQLPVAVRQAWKTM
                     VTGRPGPVVLDVPFDMFNEPIPEETPRPEDWNANISCRCGADPEGVSRAVDMLMSAQR
                     PVILVGQGVRYGRATRELLALAEKLRIPVAMSASGIGAIDSTHPLALGLVARNGPYQA
                     NHAARQADVLLALGVRFDDRTSSSWIPGFSFTIPPTKLIHVDIDPDEIARNYPVALGL
                     MADVRTFLRQLLAEIDTRLGRGQVPAERGAWLDAIAGYRKEWEDFIAPGFKADTTPIA
                     PQRAAHEIDRVLPEDAILVSDIGVHHNWLIQFCKPKRPDSLIGSMGFGPMGFGVAGAL
                     GAKLAAPGRPCVAVVGDGAFLMHASVLGTAYEYDIPVVYLVWNNYAFASIRGLQRGYL
                     GGRELATDFKHPGTNKPYNPDFAAMARAAGITGVSVGRASDLADAVREGIASGKPCVI
                     DANIDGDLNPAGAGIWELPGMGRS"
BASE COUNT          255 a          538 c          534 g          272 t
ORIGIN      
        1 cgcgggtttc atggccgacg tgtattaccg cgtgtccggc aagccgaccg cgacgtttac
       61 ttcctgcgga cccggctcgg ctaacctgcc gatcgctctc gccaattcgt tcttcgattc
      121 cgtgccgttc ctcgcggtaa cgggcaacgt cccgaccagc cagttcaact gcggcgcgtt
      181 ccaggaaacc taccggcact atcaggcgga ctatccgtcg acggtgcgcg cgtactgcaa
      241 acgcgtgttc cagcctacaa ccgcggcgca actgccggtc gcggtgcgcc aggcgtggaa
      301 gaccatggtg accgggcgcc ccggtccggt cgtgctcgat gtgccgttcg acatgttcaa
      361 cgagccgatc ccggaagaaa cgccgcggcc cgaggattgg aacgccaaca ttagctgccg
      421 ctgcggcgcc gatccggaag gggtttccag ggcggtcgac atgctgatgt cggcgcagcg
      481 cccggtcatc ctcgtcgggc agggcgtgcg ctatggccgc gcgacccggg aactgcttgc
      541 gcttgcggag aaactgcgga tcccagtcgc tatgtccgcg agcggcatcg gcgccataga
      601 cagcacgcat ccgctggcgc tcggcctcgt ggcgcgcaac ggtccgtatc aggcgaacca
      661 tgccgcgcgg caggccgacg tgctgctggc gctcggcgtg cgcttcgacg accgcacgtc
      721 gagttcctgg ataccgggct tctcgttcac catcccgccg acgaagctga tccacgtcga
      781 catcgatccg gacgagatcg cgcgcaacta tcccgtcgcg ctgggcctga tggctgacgt
      841 gcgcaccttc ttgaggcagc tgctcgccga gatcgacacg cgcctgggtc gcgggcaggt
      901 gcccgcggag cgcggcgcct ggctcgacgc gatcgcgggc taccgcaagg agtgggagga
      961 tttcattgcg cccgggttca aggcggacac caccccgatc gcgccgcagc gcgcggcgca
     1021 tgagatcgac cgtgtcctgc cggaggacgc aatccttgtg agcgacatcg gcgtgcacca
     1081 caactggctc atccagttct gcaagccgaa gcgcccggat tcgctgatcg gctcgatggg
     1141 ctttggcccg atgggctttg gcgttgccgg cgcgctgggt gcgaagctcg ccgctcccgg
     1201 ccggccgtgc gttgccgtag tcggggacgg cgcgttcctg atgcacgcca gcgtgctggg
     1261 gaccgcttac gagtacgaca tacccgtcgt ctacctggta tggaacaact acgctttcgc
     1321 ttccatccga ggcctccagc gcggctatct cggcgggcgc gagcttgcca ccgatttcaa
     1381 gcaccccggc accaacaagc cttacaaccc ggatttcgcc gcgatggcgc gcgcggcagg
     1441 catcacgggg gtgagcgtgg gccgcgcgtc ggatctcgcc gatgcggtgc gtgaaggcat
     1501 cgcaagcggc aaaccatgcg tgatcgacgc caatatcgac ggcgacctga atcctgccgg
     1561 ggcggggatc tgggagctgc cgggcatggg ccgcagcaa
//
LOCUS       DBNGWZ010000088         4585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_133669, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000088 DBNGWZ010000000
VERSION     DBNGWZ010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_133669"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16CX_02675"
     CDS             <1..335
                     /locus_tag="AC16CX_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3011446.1"
                     /translation="FTRFQQAQGELSSALSRLMVIVENYPQLKSDANFRDLQAQLEGT
                     ENRITVARNRYIKAVQSYNTVVVQFPTNLTAMIFGFKEKPQFAVENEKAIAQPPKVDF
                     GALKPAPK"
     gene            591..1439
                     /locus_tag="AC16CX_02680"
     CDS             591..1439
                     /locus_tag="AC16CX_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011447.1"
                     /translation="MKTAVNRLRGVERDIVQAPQPGDIAPYYEPQGNEIAVFEAAYKK
                     RLPVMLKGPTGCGKTRFLEFMAYQLKRPLVTVSCHEDLTSSDLVGRFLLEGAATVWQD
                     GPLTRAVKAGAICYLDEIVEARTDSTVVIHSLTDHRRKLTIEKKGQEIEAHEDFMLVI
                     SYNPGDQTVLKDLKPSTKQRFIAIHFDFPPEEIERKIVMNEAPGIASEFVHQLVTAGR
                     KARLLKDHGLEEAASTRALVYAANMINAGLDPIAACQVAMVNPITDDEELAEALMEVV
                     QAHFGS"
     gene            1456..3966
                     /locus_tag="AC16CX_02685"
     CDS             1456..3966
                     /locus_tag="AC16CX_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HIR3011448.1"
                     /translation="MTRSTQKAIEEQLAQLRDSSFVAHREIEQALPAIRPHGEDVALA
                     WILACRKLYDYDREAGKAFIRGSLEAERISETVAPWTEQALQFTRWRGAWRALEGFMT
                     NLPRAYGSLGHAGQRRWAEIGFVWCARQIDSGCAYFATSVVDLSGRQGITGIEQLTQP
                     AEELFESRKLLLATYLAGAIRVRNLLGPQAILPWALRGADIMQSGRARGETYFRLESE
                     ESLALLLDQLPGFRLVERNRLLSMLLDVWYGGLFDLREGSWSPEKGRPFVETDGRGIY
                     FPAVMPNRDEAVLALLHTAGHMRFGTFDRLAMKELFGAAGVDFPESGPVSWAPLYARY
                     GDDALRFQLIFDLCEDLRVDCRIQRLVPNYLQRMLATARPGARHPETGIYYGLAVESV
                     EQALAASRNDGVRDERFAPLLHPSATVADAWRIATAIYTEDGLPPVTDLEAFHAAYLP
                     GRGPNTTRLAHPQQQDQQQQQTQSGAEGEQRPQESGEEQDQSPQNAESGDQRDGGRKQ
                     EITGFGDSSGASTRASGRSEDQRQNSASSDKGKPYPEWDYREARYKRNWSWVQEKKLA
                     ESNITETNRLMNQYAMALKRLKKAIQAQKPTRLAPRLRQLDGEDMDLNAVVGYVVEKK
                     AGRSPKPAIYRRREMRQREIAVTLLADMSTSIMQHLPEGGGRLVDRVRAGVLLFAESM
                     EEVGDPYSIAGFCSKYRDNVSYYAIKSFEQPLSQEIRSQIGGMSGRLATRMGAAIRHA
                     TACFERIESRRRLLLILSDGRPEDYDDGGDRRYLHEDTRMAVKEAVSKGVHPFCITVD
                     TMANQYLPQIFGRGHYLVLDHINSLPNKLPEIYFRLRR"
     gene            complement(4234..4398)
                     /locus_tag="AC16CX_02690"
     CDS             complement(4234..4398)
                     /locus_tag="AC16CX_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HIR3011449.1"
                     /translation="MERVRGAIRARHYSRRTEASYVHWIKRYIYFHDKRHPEEMGASE
                     VTAFLTAGAE"
BASE COUNT          870 a         1470 c         1487 g          758 t
ORIGIN      
        1 cattcacgag attccagcag gcgcaaggcg agctttcttc cgcgctgtcc cgcctgatgg
       61 tgatcgtgga gaactacccg cagctcaaat cggacgccaa tttccgggac ctccaggcgc
      121 agctcgaggg caccgagaac cgcatcacgg tggcgcgtaa ccgctacatc aaggcggtgc
      181 agtcgtacaa caccgtcgtc gtgcagtttc ccaccaacct cacggcgatg atcttcggct
      241 tcaaggaaaa gccgcagttc gcggtcgaga atgaaaaggc gatcgcgcaa cctcccaagg
      301 tggacttcgg cgcgctcaag cccgcgccaa aataggattg aggatccaga attgaggatt
      361 gagcagatag gattgaggat tgggtgagcc gccgggagtg taacggtttg ccttgggtgc
      421 tcaatccctg atcctcaatc ctcggtcctc gctgctcaat cctgaattcc ggggcctgtc
      481 tggttgtcca atagcgaggg caggtttccc tcccccgcgc ggtgtcctac aatagacata
      541 agccaccgat gttgaagggt tttcttggcg gaattgacct tgaggtttag atgaagacag
      601 cagtgaatcg cttgcgtgga gtggagcgcg acatcgtcca ggcgccgcag ccgggcgaca
      661 tcgcgccgta ctacgagccg cagggcaacg agatcgcggt gttcgaggcc gcctacaaga
      721 agcggcttcc ggtgatgctg aaggggccca ccggctgcgg caagacgcgc ttcctcgaat
      781 tcatggcgta tcagctgaag cgcccgctcg tcaccgtatc gtgtcacgag gacctcacca
      841 gctcggacct ggtcggccgc ttcctgctgg agggcgcggc gaccgtgtgg caggacggcc
      901 cgctcacgcg cgcggtgaag gccggcgcga tctgctacct cgacgaaatc gtggaggcgc
      961 gcaccgactc cacggtcgtc atccattcgc tcaccgatca ccgccgcaag ctcacgatcg
     1021 agaagaaggg ccaggaaatc gaggcgcacg aggatttcat gctggtgatc tcctataacc
     1081 cgggtgacca gacggtgctg aaggacctca agccctcgac caagcagcgc ttcatcgcca
     1141 ttcatttcga ttttcccccc gaggaaatcg aacgcaagat cgtcatgaac gaggcgccgg
     1201 gcatcgcttc ggaattcgtg caccagctcg tcaccgccgg ccgcaaggcg cggctgctga
     1261 aggaccacgg tctcgaggag gcggcctcca cgcgcgcgct ggtgtacgcc gccaacatga
     1321 ttaacgccgg gctcgacccc atcgccgcgt gccaggtggc gatggtcaat ccgatcaccg
     1381 acgacgagga gctcgccgag gcgctgatgg aggtcgtcca ggcgcacttc ggttcatgag
     1441 aacgcccgcc tcgggatgac ccgctccacg caaaaggcga tcgaggaaca gctcgcgcag
     1501 ctgcgcgact cgagcttcgt cgcgcaccgc gagatcgagc aggcgcttcc cgcgattcgc
     1561 ccgcacggcg aggacgtggc gctcgcatgg atcctcgcct gtcgcaagct ttacgactac
     1621 gaccgggaag cgggcaaggc cttcatccgc ggcagcctcg aggcggagcg gatttccgag
     1681 acggtcgcgc cgtggaccga gcaggcgctg cagttcacgc gctggcgcgg cgcgtggcgc
     1741 gcgctcgaag ggttcatgac gaacctgccg cgcgcctacg gctcgctcgg ccacgccggg
     1801 cagcggcgct gggccgagat cggcttcgtc tggtgcgccc gccagatcga cagcggctgc
     1861 gcctatttcg cgacttcggt ggtggacctt tccgggcggc agggcatcac cggcatcgag
     1921 cagctcaccc agccggcgga agagctgttc gaatcgagga aactcctcct cgcgacttat
     1981 ctcgccggtg cgatccgcgt gcgcaatctt ctcggccccc aggcgatcct gccgtgggcg
     2041 ctgcgcggcg ccgacatcat gcaatcgggg cgcgcgcgcg gcgagaccta tttccggctg
     2101 gagtccgagg agagcctcgc cctgctgctc gaccagctgc ccggcttccg gctggtggag
     2161 cgcaaccgcc tgctctcgat gctgctcgac gtctggtacg gcgggctgtt cgatttgagg
     2221 gaaggcagct ggtctcccga gaaagggcgc ccgttcgtcg aaacggacgg ccgcggcatc
     2281 tacttccccg cggtgatgcc gaaccgcgac gaggcggtgc tggcgcttct gcatacggcg
     2341 gggcacatgc gcttcggcac ctttgaccgc ctcgcgatga aggagctgtt cggagcggcg
     2401 ggcgtggatt ttcccgaatc aggcccggtg tcgtgggcgc cgctctacgc gcgctacggc
     2461 gacgatgcgc tgcgcttcca gctgatcttc gacctgtgcg aggacctgcg ggtggattgc
     2521 cgcatccagc ggctggtgcc gaattacctg cagcggatgc tcgccaccgc gcgcccgggc
     2581 gcgcgccatc cggagaccgg catctactat ggtctggcgg tggagagcgt cgagcaggcg
     2641 ctggcggcga gccgcaacga cggcgtgcgc gacgagcgct tcgcgccgct gctccatcct
     2701 tcggccacgg tggccgacgc ctggcgcatc gccaccgcga tctacaccga ggacggtctc
     2761 ccgcccgtga ccgacctgga ggcgttccac gccgcctacc tcccggggcg cggccccaac
     2821 acgacgcggc tcgcccaccc gcagcagcag gatcagcaac aacagcagac ccagtccggg
     2881 gccgagggcg agcaacggcc gcaggagagc ggcgaggaac aggatcaatc gccgcagaac
     2941 gcggagagcg gcgaccagcg cgacggcggc aggaagcagg aaatcaccgg ctttggcgat
     3001 tccagcgggg cctcgacccg cgcctccggc cgctcggagg accagcggca gaacagcgcg
     3061 tcttccgaca aggggaagcc gtacccggag tgggattacc gcgaggcgcg ctacaagcgc
     3121 aactggagct gggtacagga gaagaagctc gcggaatcga acatcaccga gaccaaccgc
     3181 ctgatgaacc agtacgccat ggcgctgaag cgcctgaaga aggcgatcca ggcgcagaag
     3241 ccgacgcggc tcgcgccgcg actgaggcag ctcgacggcg aggacatgga tctcaacgcg
     3301 gtggtgggct acgtcgtcga gaagaaggcc gggcgctcac ccaagcccgc gatctaccgc
     3361 cggcgcgaga tgcgccagcg cgagatcgcg gtgacgctgc tcgccgacat gtccacctcc
     3421 atcatgcagc acctgccgga gggcggcggg cgcctggtgg accgggtgcg cgcgggggtg
     3481 ctgctgttcg ccgagagcat ggaggaggtg ggcgatccat actcgattgc cggcttctgc
     3541 tccaagtacc gcgacaacgt gagctactac gccatcaaga gcttcgagca gccgctctcg
     3601 caggagatcc gcagccagat cggcggtatg tccggccgcc tcgccacgcg catgggcgcg
     3661 gcgatccgcc acgcgaccgc ctgcttcgag cgcatcgaga gccggcgccg gctgctgctg
     3721 atcctctccg acggccgtcc cgaggactat gacgacggcg gcgaccggcg ctacctgcac
     3781 gaggataccc gcatggcggt gaaggaagcg gtgtcaaaag gggtgcaccc gttctgcatc
     3841 accgtggaca ccatggcgaa ccagtacctg ccgcagatct tcgggcgcgg gcactatctc
     3901 gtgctcgacc acatcaactc gctgccgaac aagctgcctg aaatttactt tcgtctcagg
     3961 cggtgaatgg aaccgcagat aaacgcagat acacgcggat aaagccgaaa acaatcagcc
     4021 agagagaaca cagaggacac agagacataa gacgcaagaa cacgaatggg cacgcaggcg
     4081 tgcccgttta ttttttccgg gggtgttgaa gggagtctcc gccgttcgac gcgcgggctt
     4141 atgaggggag ggcgacggct actgggtatt ccgccgtgat gtcccgagcg atgggcggct
     4201 ggctagtccc gctacccgca tcgaacgggc tcttcactcc gcgcccgcgg tcagaaacgc
     4261 agtgacttcg cttgccccca tctcctcggg atgtcgcttg tcatggaagt agatgtaacg
     4321 cttgatccaa tgcacgtacg aggcttctgt gcgcctgctg taatggcggg cgcgtatggc
     4381 accgcgcacc ctctccatca atcgaggttt ggcggcattt tgacaaatcg cagatgaatc
     4441 aacgttgtcg cccatattac gccggataac aatatcccca cgtcctttca cctcagacaa
     4501 cgcgttgatt tggaatgcgg ttgacagcgt caatccgatg aagcgaccat gttatacggc
     4561 atctttgccg gatatttata gttag
//
LOCUS       DBNGWZ010000089         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1770923, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000089 DBNGWZ010000000
VERSION     DBNGWZ010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1770923"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1516)
                     /locus_tag="AC16CX_02695"
     CDS             complement(122..1516)
                     /locus_tag="AC16CX_02695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3011450.1"
                     /translation="MGFSQFKLHPQITAGVKAQGYDTPTPIQRQAIPPVLEGHDVMGL
                     AQTGTGKTAAFVLPILERLLQGPRGRVRALIIAPTRELAEQIHTEIGKLGLQTRLKSV
                     TIYGGVGLNPQIQKLRAGVEIVVACPGRLLDHINQRTIDLSHVEALVLDEADRMFDMG
                     FLPDIRRIIKHVPAKRQTLLFSATMPDDIRKLAHEVLRAPVTVQVGHTAPANTVSHAL
                     YPVDQHLKTALLLELLKHTDTGSVLIFTRTKHRAKTVGRKLEQAGYRAASLQGNLSQN
                     KRQAALDGFRSGSHQILVATDIAARGIDISSISHVINYDMPDTTDAYTHRIGRTGRAA
                     KTGDAFTFATGEDEAIVRSIERVLGEKIERRTVKGFDYKKPAPARDAEFARPPREPQR
                     RREQKRPEQASHRAASSPQKAHHTQPAHGGAHRTAAAQPSHGQGTGHRGQATQHPTGS
                     TAYKSRTPKPRTYR"
BASE COUNT          287 a          522 c          600 g          391 t
ORIGIN      
        1 agcgttcctt cacgtactcc tcgagcagtt tcagaaatgc cgcgtcatcg tcgatcagga
       61 gaattttttt catcggaaag ttgttttttc cgtccccgca taggtgtttt gccggatgct
      121 tttaacgata agtccggggc ttaggcgtcc ggcttttgta agccgttgat cctgtgggat
      181 gctgcgttgc ttggccgcga tggccggtcc cctgcccgtg cgaaggctgg gccgctgccg
      241 tacgatgggc gccgccgtgg gcgggctggg tgtggtgcgc cttctgcggc gaagacgccg
      301 cccgatgcga cgcctgttcc ggccttttct gctccctgcg gcgctgcggc tcgcgcggcg
      361 ggcgggcaaa ctccgcgtcg cgcgccggcg cgggcttttt ataatcaaag ccctttaccg
      421 tgcggcgttc gatcttttcg ccgagcacgc gctcgatgct cctgacgatg gcttcgtcct
      481 cgcccgtggc gaaggtgaag gcgtcgccgg tcttggcggc ccgtccggtg cggccgatcc
      541 ggtgcgtgta ggcgtcggtg gtgtcgggca tgtcgtagtt gatgacgtgg gaaatgctgg
      601 agatatcgat gccgcgggcc gctatatcag tcgccacgag gatctgatga gagccgctgc
      661 ggaatccgtc gagggcggcc tggcgcttgt tctgcgacag gtttccctgg agcgacgcgg
      721 cccggtaccc tgcctgttcc agcttccggc caacggtctt cgcgcggtgc ttcgtgcgcg
      781 tgaagatcag cacggagccc gtgtccgtat gtttcaacag ttccaagagg agcgcggtct
      841 tgaggtgctg gtccacgggg tagagagcgt gggacacggt gttcgcgggc gcggtgtggc
      901 cgacctgcac ggtgaccggg gcgcgcagga cttcatgggc cagtttccgg atgtcatcgg
      961 gcatggtggc cgagaaaagc agggtctggc gcttcgcggg cacgtgcttg atgatcctgc
     1021 ggatgtcggg caggaacccc atgtcgaaca tgcggtccgc ctcgtcgagg acgagggcct
     1081 ccacgtgcga caggtcgatg gtgcgctggt tgatatggtc gagcagacgg ccggggcagg
     1141 cgacgacgat ctcgaccccg gcgcgcagct tctggatctg cggattcaag cccacgccgc
     1201 catagatcgt gacgcttttg aggcgtgtct ggagtcccag cttgccgatc tcggtatgga
     1261 tctgctcggc gagctcgcgc gtcggcgcga tgatcagggc gcggacccga ccgcgcggcc
     1321 cctgcagcag gcgctccagg atcggcagca cgaaagccgc ggtcttgccg gtcccggtct
     1381 gagcaaggcc catcacgtca tggccttcaa ggaccggcgg aattgcctgg cgctggatcg
     1441 gggtcggagt gtcgtagcct tgcgccttga cgccggccgt tatctgggga tggagtttga
     1501 attgagaaaa gcccacgaat acctcgctaa gataaaatta taattttata ttgatcagcc
     1561 acggaaggac actgataaag cgggaacaaa cggtcgttaa tttccacgat tgcagccgat
     1621 aaacgccgta cttatcttcc tctgcgtcct ctgcggtgat aacttccggt cttcggctaa
     1681 ttggccgaag ccatcagccg ctgcctgtcc tcgaacagca catcgcactt gttctcatag
     1741 agcttctgca cgacgccctt gccgaaccgg tcgtggacga cgatatcgcc gatgcggtat
//
LOCUS       DBNGWZ010000090         1684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1141874, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000090 DBNGWZ010000000
VERSION     DBNGWZ010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1141874"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="AC16CX_02700"
     CDS             <1..1568
                     /locus_tag="AC16CX_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3011451.1"
                     /translation="LFSFANPANERAVAEALKPLRLPVSLSHLVLPEFREYERASTVV
                     VNAYLQPVMARYLEKLEERLRAIARRRAGVYVMQSSGGITSLASAAREPVRTVLSGPA
                     GGVVGAAAMARRSGFARIISFDMGGTSTDVALVDGSPRAGNEGELAGLPVRVPMLDIH
                     TVGAGGGSLARFDAAGALRVGPESAGADPGPICYGRGDQPTVTDANLLLGRLHPRRFL
                     GGEFALDLPRTRKLVRAWLRRRGSPLSPEQFAAGVVRVVNAAMEKAIRVVSIERGYDP
                     RDFTLVAFGGAGGMHSCELARALAIPRVIVAALPGALSAYGILVSDIVKDYSRTVLWR
                     ATRVEQDLARRMQDEFVQLRRAAEADVQRERWAGTIRHELALDVRYRGQGYELNVPFT
                     NDWLRRFHVQHARRYGYSHAEREVEIVTLRLRARLPSPQPRWRAPSRAATLTPPELTT
                     VIFDSRALRTPLYDRAALAPGESYRGPAVITEYSATTVIPPGMRFQADVAGNLLIDVR
                     SAHDSRLPRRSEN"
BASE COUNT          231 a          593 c          570 g          290 t
ORIGIN      
        1 tgctgttctc cttcgccaat cccgccaatg aacgcgccgt ggccgaggcc ctcaagcccc
       61 tgcgcctgcc ggtctcgctt tcgcacctgg tgctgccgga gttccgcgag tacgagcgcg
      121 ccagcaccgt cgtcgtgaac gcctacttgc agccggtgat ggcgcggtac ctggagaagc
      181 tggaggagcg gctgcgcgcc attgcccgtc gccgggcggg cgtgtacgtc atgcagtcga
      241 gcggcgggat tacctcgtta gcctcggccg cgcgcgagcc ggtgcgcacc gtgctttccg
      301 ggcctgcggg tggagtggtg ggcgcggccg ccatggcgcg ccgcagcggc ttcgcgcgca
      361 tcatctcctt cgacatgggt ggcacctcca ccgacgtcgc cctggtggac ggctcgccgc
      421 gcgccggcaa cgaaggcgaa ttggccggct tgccggtgcg cgtccccatg ctcgacatcc
      481 acaccgtggg cgcgggcgga ggttcgctcg cccgcttcga cgccgcgggc gcgctgcgcg
      541 tggggccgga atctgcgggc gccgatccgg gcccgatctg ctacggccgc ggcgaccagc
      601 ccaccgtcac ggacgccaac ctgctgctcg gccgtctgca tccgcggcgc ttcttgggcg
      661 gcgaattcgc cctcgatctg ccgcgcaccc ggaagttggt gagggcttgg ctgcgccggc
      721 gcggctcgcc gctttcgccg gaacagttcg ccgccggcgt cgtccgcgtg gtcaacgcgg
      781 ccatggagaa ggccatccgc gtggtctcca tcgagcgcgg ctacgatccc cgcgatttca
      841 ccctggtggc cttcggcggc gccggcggca tgcactcctg cgagctggcg cgggcgctgg
      901 ccatcccgcg cgtgatcgtg gctgcgctgc cgggcgcgct ctccgcctac ggcatcctgg
      961 tcagcgacat cgtcaaggac tattcgcgta ccgtcctctg gcgcgccacc cgcgtcgagc
     1021 aggaccttgc gcgccgcatg caggatgaat ttgtgcaact ccgccgcgcc gccgaggcgg
     1081 atgttcaacg cgagcgctgg gcaggaacga ttcgccatga gttggccttg gacgtgcgct
     1141 accgcgggca gggatacgaa ttgaacgtgc ccttcaccaa cgactggctg cgacggttcc
     1201 atgtgcaaca tgcacgccgc tacggttaca gccatgcgga gcgggaggtg gagatcgtga
     1261 ccctgcggct gcgcgcccgc ctgccttcgc cccagccccg ttggcgtgcg ccttcgcgtg
     1321 ccgcaactct aacgccgccg gagctgacca cggtgatatt cgacagcaga gcgctgcgca
     1381 ccccgctgta cgaccgcgcg gcgctggctc cgggcgagag ctaccgtgga cccgcggtga
     1441 tcaccgaata cagcgccacc accgtgattc caccgggcat gcgcttccag gcggacgtcg
     1501 ccggcaactt actgattgat gtcaggtctg cccatgactc aagattgcca aggcgttctg
     1561 agaactgaga acttgagagt agccatccaa ggcgaattgg gttcgttcag ccacgaagcg
     1621 gcacgcctgt tcctgcccgg ctgccgggtc gcgccttgtg tgacctcgtc cgaggtgttc
     1681 gacc
//
LOCUS       DBNGWZ010000091         5757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3156941, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000091 DBNGWZ010000000
VERSION     DBNGWZ010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3156941"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1901
                     /locus_tag="AC16CX_02705"
     CDS             318..1901
                     /locus_tag="AC16CX_02705"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3011452.1"
                     /translation="MVTSRQKIIGAAVLTAVLTAGAGAYAWWGARNAATDYRFSKVER
                     GNITSVVSATGTLNPVVSVQVGSQVSGQIKEILVDYNSPVKKDQVIARIDPDTFALRV
                     NQAMADLEATRASVLTQRANVAALQAEVSRAKVNVADAERDFQRNKMLFEKNFVSAAA
                     LDKAQFVYQASQEQLKTAQAQMAVGEAQVRNVEAVVKQRESQLAQAKVDLERTTIRAP
                     VDGIVVKKSVEPGQTVAASLQAPELFVIAQDLRQMQVETSIDEAEVGRIGVGQTAIFT
                     VDSFPGRTFRGTVSQVRKAALVVQNVVTYTAVIATSNPNLELFPGMTANVRIVVNTRE
                     NALKVPNAALRFRPSGVTEPREAGGARDATAAASAPEAAGKGRGGQQDQAVRERLTKE
                     LNLNAEQQAMLETIQNETRDRIGALNVEDPDERRRQAGRLRAESRARIAEMLNLEQRK
                     RYEETSPGRAGGSVTGGRVWILDDTGKPRAVSVRIGLSDGTYSEVIGGLQEGAQVIIG
                     TQADAKAARSQTKGGPRFF"
     gene            2004..2744
                     /locus_tag="AC16CX_02710"
     CDS             2004..2744
                     /locus_tag="AC16CX_02710"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011453.1"
                     /translation="MADPLIVTRGLVKEYRMESYTVAALRGVSLEIGPGEFMAVMGPS
                     GSGKSTFMNLLGCLDSPTSGEYLLAGERLSELSGDALAAIRNRRIGFVFQNFNLLPRT
                     SAVENVELPLLYSHCAAEERRRRAMTRLAEVGLAERAHHHPQQLSGGQQQRVAIARAL
                     VNNPVLILADEPTGALDTRTSFELMGLLQQLNRGGMTVVLVTHERDIASFASRIVSFR
                     DGHVVDDQRVARPADAQAMLREFEKAAA"
     gene            2741..3970
                     /locus_tag="AC16CX_02715"
     CDS             2741..3970
                     /locus_tag="AC16CX_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011454.1"
                     /translation="MNWLASIRIALRALRVNKLRSTLTMLGIIIGVAAVITMIAVGAG
                     AQARVEEQIKGLGTNLIMLLPGSVTSGGARMGSGTRVSLTDDDAYAIQREVAQVQVAA
                     PQLRGGGQVVAGGNNWSTIFYGVTPEYFEARNWVIASGKGFEPGDLAGSAKVALLGET
                     VVRNLFGDSDPIGQVIRIRKVPFTVIGTLERKGQSLMGYDQDDVILMPITTARNRVLG
                     GGGRQRAAGTIFVKVREDADMAETENQIRNLLRQRHRIQPGQDDDFTLRNLAEVLAAR
                     EESSRIMTLLLAAVASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARARDILTQF
                     LVEAVTLALIGGLLGIVLGVGGSFAIGHFADWHTELDADAIALAVGFAAAVGVFFGFY
                     PARKASRLLPIEALRYE"
     gene            4095..4916
                     /locus_tag="AC16CX_02720"
     CDS             4095..4916
                     /locus_tag="AC16CX_02720"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR3011455.1"
                     /translation="MDIALYLNAFVLGVVEGLTEFLPISSTGHLIIAGQLLGFGGDKA
                     KVFDIVIQSGAMMAVIWEYRTRFTRMLTGLSSDPAARRFAANLVIAFVPAALLGLAFG
                     SAIKQHLFHAVPVAAAFIAGGLIILWVERSPLRTMVEDVDAMTWKDALKVGLAQSFAL
                     IPGTSRSGATIIGGMLFGLSRRAATEFSFFLAVPTLIAAGAYDFYKHRALFEAADLGL
                     FAVGFVISFVSAFLCVRWLLRYIATHDFGAFAWYRIVFGIIVLATAYTGLVNWSA"
     gene            complement(5005..5454)
                     /locus_tag="AC16CX_02725"
     CDS             complement(5005..5454)
                     /locus_tag="AC16CX_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3011456.1"
                     /translation="MAVTDILQNAAERAQKMGLPYSGALTPAEAHEVWRNAPGAQLVD
                     VRTRAEWDYVGRIPGAVEIELLTYPGNRPNTAFLAELEDRVGKDVPVMFICRSGGRSH
                     NAALLATQAGYRDCYNVLEGFEGDKDAQSHRNSTGGWRVAGLPWTQG"
BASE COUNT         1020 a         1806 c         1883 g         1048 t
ORIGIN      
        1 tcaaccgtcg cgcggaggcg atcgccgccg agctgaagac acgcgggacg ctggctggac
       61 tgctgctcct caccgcgttc gaggcggcgg ctaaacaggc gttgcgcgaa gctctggccg
      121 agcccgctcc cgcggcacgc tgaaaccttt tgttacaccc gcaggcccgc gccatgagca
      181 ggcttgcggc acctccgcta caatccttca ctctaaagcg gtgagcgata agcggtaaac
      241 ggtcaacggc cggagatggt cgggggaccg ggttccgtgc agtcggctca ttggaattcc
      301 gctcaccgct aacgagcatg gtgacatcac ggcagaaaat catcggcgcg gcggtcctca
      361 ccgcggtatt gaccgcgggt gccggcgcct acgcgtggtg gggcgcgcgt aacgccgcga
      421 cggactaccg cttcagcaag gtcgagcgcg ggaatattac ctcggtggtc tcggcgaccg
      481 gtaccctcaa tccggtggtt tcggttcagg ttggctcgca ggtctcgggg cagatcaagg
      541 aaatcctcgt tgattacaac tcgcctgtga agaaggacca ggtcatcgcg cgcatcgatc
      601 cggatacctt cgcgctgcgt gtgaaccagg caatggcgga cctggaagcg acgcgcgcca
      661 gcgtgctgac gcaacgcgcg aacgtcgccg cgctgcaggc cgaagtgtcg cgcgccaagg
      721 tcaacgtggc cgacgccgag cgcgatttcc agcgcaacaa gatgctgttc gagaagaact
      781 tcgtatcggc ggcggcgctc gacaaggcgc agttcgtcta ccaggcgtcc caggagcagc
      841 tcaagaccgc gcaggcgcag atggccgtgg gcgaagccca ggtgcgcaac gtcgaagcgg
      901 tggtcaagca gcgcgagtcg cagctcgcgc aggcgaaagt ggatctcgag cgcaccacca
      961 tccgtgcgcc cgtggacggc atcgtggtga agaaatcggt cgagccgggg caaacggtgg
     1021 cggcgagcct gcaggcgccg gagctcttcg tgatcgccca ggacctgcgc cagatgcagg
     1081 tcgagacctc gatcgacgag gcggaagtgg gccgtatcgg tgtcggccag acggcaatct
     1141 tcaccgtgga ttcgttcccc ggcaggactt tccgcggcac cgtttcgcag gtgcgcaagg
     1201 ccgcgctggt ggtgcagaac gtcgtgacct ataccgcggt catcgccact tccaatccca
     1261 acctggaatt gttcccgggc atgactgcca acgtacgcat cgtcgtgaac acgcgcgaga
     1321 atgccctcaa agtgccgaac gcagcgctgc ggttcaggcc gtcgggcgtg accgagccgc
     1381 gcgaggcggg cggcgcgcgt gatgccacgg ccgcggcatc tgcgcccgaa gctgccggca
     1441 agggccgcgg tggccagcag gaccaggccg tgcgggagcg gcttaccaag gagctcaacc
     1501 tcaacgccga gcagcaggcg atgctcgaaa cgatccagaa cgaaacccgt gacaggatcg
     1561 gggcgctgaa cgttgaggac ccggatgaac ggcgacggca ggccgggcgg ctgcgcgcgg
     1621 agtcccgggc gcgcatcgcc gagatgctga atctggaaca gcgcaagcgc tacgaggaga
     1681 cgtcccccgg tcgggccggc ggttcggtga cgggcgggcg ggtatggata ctggacgata
     1741 ccggcaagcc gcgcgcggtc agcgtgcgca tcgggctctc cgacggcacc tacagcgagg
     1801 tgatcggcgg tctgcaggaa ggggcccagg tgatcatcgg cacacaggct gacgccaagg
     1861 cggcgcgttc ccagaccaag ggtggtccgc gcttctttta agagcggtga ttaggtgact
     1921 aggtgactgg gtgattaagc aaaatctcaa acgccgcgcc gttactcagt cacttggtca
     1981 ctcaatcact taatcactcg actatggctg accccctcat cgtcactcgc ggtctcgtca
     2041 aggagtaccg catggagagc tacaccgtcg cggcgctgcg cggggtgtcg ctcgagatcg
     2101 gcccgggcga gttcatggcg gtgatggggc cgtcgggctc cggcaagtct accttcatga
     2161 acctgctcgg atgcctcgat tccccgacca gcggcgaata tctcctcgcc ggcgagaggt
     2221 tgtcggagct ttccggcgac gcgctggccg cgatccgaaa ccgccgcatc ggcttcgtat
     2281 tccagaactt caacctgttg ccgcgaacat ccgccgtcga gaacgtggag ctaccgctgc
     2341 tctacagtca ctgcgcggcg gaggagcggc ggcggcgggc gatgacacga cttgccgagg
     2401 tggggctcgc tgagcgtgcg catcaccatc cccagcagct ctcgggcggc cagcagcagc
     2461 gggtcgcgat cgcgcgtgcg ctcgtcaata atccggtgct gatcctcgcc gacgagccga
     2521 ccggcgcgct cgatacccgc accagcttcg aattgatggg gctgctgcag cagttgaatc
     2581 gcggcggcat gacggtggtg ctggtgacgc acgagcgcga tatcgcgtcc tttgcctcgc
     2641 gcatcgtgag cttccgcgac ggccacgtcg tggacgatca gcgtgtcgcg cgaccggccg
     2701 acgcgcaggc gatgctgcgg gaatttgaaa aggcggcggc atgaactggc tcgcgagcat
     2761 ccgcatcgct ctgcgtgccc tgcgcgtgaa caagctgcgg agcacgctca ccatgctcgg
     2821 cattatcatc ggcgtggctg ccgtgatcac catgatcgcc gtcggcgccg gcgcgcaggc
     2881 gcgtgtggag gagcagatca agggcctcgg cacgaacctc atcatgttgt tgccgggctc
     2941 ggtaacgagc ggcggcgcgc gcatgggctc cggtacccgg gtctcgctga ccgacgacga
     3001 cgcctatgcc atccagcgcg aggtcgcgca agtgcaagtg gcggctccgc aactgcgcgg
     3061 cgggggccaa gtggtcgctg gcggcaacaa ctggtccacc atcttctacg gggtgacgcc
     3121 ggagtacttc gaggcgcgca actgggtgat tgcttccggt aagggcttcg aaccgggcga
     3181 cctcgccggc tcggccaagg tcgcgctgct tggcgagacg gttgtgcgca acctgttcgg
     3241 ggacagcgat ccgatcggcc aggtcatccg catccgcaaa gtgccgttca cggtgatcgg
     3301 cacgctcgag cgcaaaggcc agagcctgat ggggtacgac caggacgacg tgatcctgat
     3361 gccgatcacc accgcgcgca accgcgtgct tggcggcggc ggcaggcagc gggctgcggg
     3421 cacgatcttc gtcaaggtcc gcgaggacgc ggacatggcc gaaaccgaaa accagatcag
     3481 gaacctgctg cgccagcgcc accggatcca gccgggccag gacgacgact tcacgttgcg
     3541 caatctggcg gaagtactgg cggcgcgcga ggagtcgagc cgcatcatga cgcttctgct
     3601 cgccgccgtg gcttcggtct cgctgctcgt tggcggcatc ggcatcatga acatcatgct
     3661 ggtgtcggtg accgagcgca cgcgggaaat cgggctgcgg atggcggtcg gcgcgcgcgc
     3721 gcgcgacatc ctgacgcagt ttctggtgga ggcggtgacg cttgcgttga ttggcggcct
     3781 gctcggtatc gtgctcggag ttggcgggtc gtttgccatt ggacatttcg ccgactggca
     3841 tactgaactc gatgcggacg ccatcgcgct ggcggtaggc ttcgcagcgg cggtcggtgt
     3901 tttttttggc ttctatccag cgcgcaaggc gtcccgccta ctgccgatcg aagcgcttcg
     3961 ctatgagtga ttaagtgact aggtgattaa gtgattgagt ggacccctga accgccacta
     4021 cctaatcact aaatcactca atcactcaat cactgtcatt cagggcgcgc atcgcaggta
     4081 gaatggcgcc ctaaatggac atagcgctct acctcaatgc cttcgtgctc ggcgtcgtcg
     4141 aaggcctcac tgaattcctg ccgatctcca gcacgggaca tctcatcatc gcggggcaac
     4201 tcctgggctt cggcggcgac aaagccaagg tcttcgacat cgtgatccag agcggcgcga
     4261 tgatggcggt gatatgggag taccgcacgc gtttcacgcg catgctcacg gggctttcga
     4321 gcgatcccgc cgcccgccgc ttcgccgcga acctcgtgat cgcgttcgtg ccggcggccc
     4381 tgctcggact cgctttcgga agcgcaatca agcaacacct gttccatgcg gtcccggtcg
     4441 cggccgcatt catcgccggc ggcctgatca tcctctgggt ggagcgctcc ccgctgcgga
     4501 cgatggtcga ggatgtggac gccatgacgt ggaaggacgc gctcaaagtc gggctggcgc
     4561 agtcgtttgc cctgataccc ggcacgtcgc gctcgggcgc gacgatcatc ggcggcatgc
     4621 tgttcgggct ttcgcgccgc gccgccaccg agttctcgtt cttccttgcc gtgccgacct
     4681 tgatcgccgc gggggcgtac gacttctaca agcaccgcgc actgttcgag gcggccgacc
     4741 tcggcctgtt tgccgtcggc ttcgtgatct ctttcgtttc ggcctttctc tgcgtgcgct
     4801 ggctgctgcg ctacatcgcc acccacgact tcggcgcgtt cgcctggtac cgcatcgtgt
     4861 ttggcatcat cgtgctcgcc acggcgtaca ccggcctggt gaactggtcg gcgtaacgta
     4921 ttaaggcaga aggcagaagg cagaaggcag aaggcgacaa caacctactt ctgggttttt
     4981 tccttcatcc ttccgccttc ctccttagcc ttgcgtccat ggcagtcccg cgacccgcca
     5041 gccaccggtt gagttgcgat ggctctgtgc gtccttgtcg ccttcgaagc cctcgagcac
     5101 gttgtagcaa tcgcggtaac ccgcttgcgt cgcgagcaac gccgcgttgt gcgagcggcc
     5161 gccgctgcgg cagatgaaca tgaccgggac atcctttccc acccggtcct cgagttcagc
     5221 gagaaacgcg gtattgggcc ggttgccggg ataagtcagc agctcgatct ccaccgcgcc
     5281 ggggatgcga ccgacatagt cccattcggc gcgggtgcgc acgtccacaa gttgcgcgcc
     5341 cggcgcattc cgccatacct cgtgcgcctc ggccggcgtg agcgcaccac tgtatgggag
     5401 ccccattttt tgtgctcttt cggcggcatt ctggaggata tctgtgacgg ccatggaggt
     5461 tccttggggg tcacgccgac atcgctgtcg gcattcgcac ccgatagcgt atgcggctgg
     5521 aaagtaagca ttattttaca tcggtatcta ccaggcattc tatgcaccaa tacagtgcgc
     5581 gctggataat tgagcgcaat cttggtgcat cggagcttgt atgggggaaa caaacccctt
     5641 gtggcacaat gcgtttcgca caaaaaccct tggcacaatt cctgctcaac aggaagaccc
     5701 gattcaccgt attttcgttt cacaccaatc aacttcagga gagctagatg tcaaccg
//
LOCUS       DBNGWZ010000092        19414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_511956, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000092 DBNGWZ010000000
VERSION     DBNGWZ010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19414
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_511956"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16CX_02730"
     CDS             complement(<1..278)
                     /locus_tag="AC16CX_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011457.1"
                     /translation="MTHAEFTAAYARGEVKIEVDPQGAARFLSARLLLPFVVMPVLGV
                     GVALALIGWILTGLATLALGIVVPRLIKRSAPHFLFQQVLQDEAVYEEV"
     gene            complement(275..1309)
                     /locus_tag="AC16CX_02735"
     CDS             complement(275..1309)
                     /locus_tag="AC16CX_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3011458.1"
                     /translation="MPLQLPAETPAQIAWRFGRALALLALLHVVGTVGFRVLAGPQYS
                     WLDCLYMTFITIATIGYGEVVDLSSNPLGRVFAMAIGFAGIANTWYIMSLMVSYLVEG
                     KINEALWRRRMEKHIHELKDHYIMCGIGRVGRNVANELVATHRPYVVIDEAMQSIETH
                     IEHHPDTMYIHGDGCDDDVLIRAHIESAVGVFAVTGDDSKNLLITITAKQLNPEVRVV
                     ARCHEVRNIEKIRKAGADAIVSPDFTGGLRIASAMIRPHVVTFLDEMLKSEGNLRVEE
                     IIVPAGFPETTVGSLNLKSPDYILLAVRDHQSWNFNPSGDLRVRAGSTLVVMATPEGR
                     RSLEKLVGWV"
     gene            complement(1339..1959)
                     /locus_tag="AC16CX_02740"
     CDS             complement(1339..1959)
                     /locus_tag="AC16CX_02740"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR3011459.1"
                     /translation="MRTPDYWPQATRELAERDAVLRGLIERFQGLALGSRGDAFSTLA
                     RSIVGQQISVKAAQSVWDRLTARLGPMSPATVRSARKTTLRTCGLSGQKTAYLQDLAA
                     KFLDRTLDASRWHALDDEALIGELTLVKGIGRWTAEMFLIFHLTRPDVLPLADLGLQR
                     AMRLHYNRGRPLSVARMNKIGAAWAPWRSVATWYLWRSLDPLPVEY"
     gene            complement(1956..2519)
                     /locus_tag="AC16CX_02745"
     CDS             complement(1956..2519)
                     /locus_tag="AC16CX_02745"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR3011460.1"
                     /translation="MPRKFFRRFLPSHESVRSNRHIARLGPWLQHHNLWHLHRRSVAG
                     GVAVGLFAGLIPGSNPVQFTAAALLAIAGRVNLPIAVLVTLYSNPFTIVPLYFVAFEL
                     GRFALMEGGNGLPPLAFGLEDKGISEWIPAVLHWLASVGKPLVIGLPLLALLLAITGY
                     FVVDWAWRLYVRCAWESRRRRRAQRAS"
     gene            2744..5137
                     /locus_tag="AC16CX_02750"
     CDS             2744..5137
                     /locus_tag="AC16CX_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR3011461.1"
                     /translation="MRLNIVCFVAGAWWLQQQAALPDTGWTWVLAAAGLATTVVRFES
                     VTLRLACGLCVKAACFALGFSWAAWCAQQRLADALPAEWEGRDIAVIGVVAGLPQTYE
                     RGVRFEFDIERVLTPQARVPEHIILSWWGSPARNDRPATFPPLNAGERWQLMARLKRP
                     HGTANPHGFDYEAWLLELNLRATGYVRPKGGSHRLASMVHEPKYWIERARGMIRARIQ
                     AALPDAPYVGVIAALAIGDQRAIPPEQWQTFTRTGVNHLMSISGLHVTMVSGLVFALV
                     YGLWRRVPRLVLVLPALKAAALAGLGAALLYAALAGFAVPAQRTVYMLAVVAAALWLG
                     VIETASVVLCAALLVVVLLDPWAVLAPGFWLSFGAVAVIMYVSVGRIGRTRWLADWAR
                     VQIAVTLALIPPLLALFQLVSIVSPLANAVAIPVVSLVVAPLALIGAAMPFDFVLHIA
                     HLVMSGCMAFLEWLSALPDAVWQQHAPPSWSVIVAVAAVAWLLAPRGLPARWLGVVGL
                     LPLFATAPGALNAGDVEIIVLDVGQGVSALVRTANHALLYDAGPQFGPGADSGSRIIV
                     PYLRAAGVKRLDGFVVSHDHDDHWGGAASVLQAVPVAWVLTSLPDLDPLVVQAEPALR
                     CEAGQRWEWDNVRFEVLHPASASYDDPVVKDNDRSCVLRVEAPGGRALLPADIERRSE
                     EELLQRERDRLHVDVLLAPHQGSRTSSTPAFVQAVSPRIVVFPVGYRNRFGHPHREVV
                     ERYRNIGTRVYRTDRDGAVTISIRPEGTISVTSYRAFYRRYWQTVLEGDPAPDPEEF"
     gene            5320..7536
                     /locus_tag="AC16CX_02755"
     CDS             5320..7536
                     /locus_tag="AC16CX_02755"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3011462.1"
                     /translation="MAKIPQLKLVHPPTPDFSTGFSEHAARLAPADRDRVHAALAVAE
                     PLLGEARLPGGEVALNHALGTAAIVSELKLDGDAVAAALLTPLAVIAPERMRTLKERC
                     GTVVAELVEGVARMAQIHALSSRGAPAARPAGQEAQLEALRKMLLAMVQDIRVVLVKL
                     ADHTQELRFAAKSDDEEAKRGTAALTTDIFAPLANRLGVWQLKWEMEDLAFRILEPET
                     YKKIARMLDEKRVDRERYIENVTALLKGELARAGIAAEVTGRPKHIFSIYKKMKIKAV
                     DFESLYDVRAVRVLVQDVTDCYTALGLVHNLWAPIPGEFDDYIAKPKSNLYRSLHTAV
                     IGPEDKALEVQIRTHEMHQHSELGVAAHWRYKEDSRRDRDYDRKIAWLRQILEWKGEA
                     GDAGELAERFRTGLFDDTIYVLTPQGRVIALPKGATPVDFAYHVHTELGHRCRGAKVD
                     GMMVPLNTPLVNGQRVEILAAKQGGPSRDWLTPALGYVMSQTARVKIRQWYNRQNHEA
                     AAAQGRAVVEKELQRQGMTGLNLEHLAAKLGYGRLDDFLAVVGRGEIGPRQLEQAMHA
                     LNPHTGVLKTEARDEAPVAPARRESPAARKGGVLVVGVDRLLTVPAKCCKPAPPDVIV
                     GFITRGRGVTIHRAACASLNRLDPERRVAAEWGEAEDATFPVDIEVDAVKRARQQREI
                     SEMLAREKIRIVGSRSVEVDAAVRLRYTLEVANLAQLSRVLALIREVRGVMRAVRR"
     gene            complement(7601..8431)
                     /locus_tag="AC16CX_02760"
     CDS             complement(7601..8431)
                     /locus_tag="AC16CX_02760"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008684 - 2-oxopent-4-enoate hydratase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3011463.1"
                     /translation="MKAKIDARKIARELLDALDSGGTIPSIAERYPVFGWDEGYAVAA
                     EILKLRRARGERTVGRKIGFTNRNIWAEYGATAPIWAHVYDRTLLRASGNRATVSLKG
                     SVRPRLEPEIAFRLKAPLPAGVDDPAQILEAVEWLAPSFEIVDCHFADWKFKPADSAA
                     DFSFHWRLVVGAPRDIRSADPPALASQLRDCRITLSRSDRVMDQGVGANALGHPAAAL
                     AHLADVLSRQLRFEPLAAGEVITTGTLTAAMPIRPGEEWSSRYEGLPGVTGLTIQFTE
                     "
     gene            complement(8480..8770)
                     /locus_tag="AC16CX_02765"
     CDS             complement(8480..8770)
                     /locus_tag="AC16CX_02765"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3011464.1"
                     /translation="MAAYVIVEMTVTDAVWIEDYRKLAGASVVAYGGKFLVRGGKTEV
                     FDGDWNPQRIVVIEFPGMQQAKRWRSSPEYGKACEIRDRAAKTRMLAVEGVA"
     gene            8852..8928
                     /locus_tag="AC16CX_02770"
     tRNA            8852..8928
                     /locus_tag="AC16CX_02770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8886..8888,aa:Val,seq:gac)
     gene            9076..10998
                     /gene="thrS"
                     /locus_tag="AC16CX_02775"
     CDS             9076..10998
                     /gene="thrS"
                     /locus_tag="AC16CX_02775"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3011465.1"
                     /translation="MPEIRLPDGSVKKFDGAVTVAELAQAIGPGLARAALAGKVNGKL
                     VDTSFRIDSDAEVAIITDKDREGLEILRHSTAHLLAHAVKELFPDAQVTIGPVIEDGF
                     YYDFAYKRPFTPEDLAAIEKRMQEITKRDIKVVREVMPRDAAVKFFRDQGERYKAEII
                     AAIPDGEAISLYRQDNFVDLCRGPHVPSTGKLRVFKLMKLAGAYWRGDSKNEMLQRIY
                     GTAWAKKEDQDIYLHRLAEAEKRDHRRLGRSLELFHTQDEAPGMVFWHPKGTVIWQQI
                     EQYMRRLLSENGYQEVRTPQVLDVSLWKRSGHWDNFRENMFFTESESRDYAVKPMNCP
                     GHVQIFNQGLKSYRDLPLRLAEFGSCHRNEPSGALHGLMRARSFIQDDAHIFCTEAQI
                     EPEVIRFIELLQRVYADFGFDEILVKLSTRPAKRVGTDAQWDKAEAALKTALDHKGLG
                     YDLQPGEGAFYGPKIEFSLKDSLGRVWQCGTMQLDFSMPERLGAEYVAEDNARRVPVM
                     LHRAILGSFERFIGILIEHYAGAMPVWLAPVQVVVMNITEHQADYAERVVSELRRAGL
                     RVAADLRNEKISYKIREHSLQKLPYQVIVGDKEKAVQQVAVRTRRGEDLGQMSLADFV
                     SRLRAEEARLGRAAQD"
     gene            11019..11654
                     /gene="infC"
                     /locus_tag="AC16CX_02780"
     CDS             11019..11654
                     /gene="infC"
                     /locus_tag="AC16CX_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR3011466.1"
                     /translation="MAQEKEPRINGEITAPEIRVIGANGEQIGILSLAAANKLAEEAE
                     LDLVEIAPMATPPVCRIMDYGKYKYRESKKRHEAKLKQKQIVVKEIKFRPGTDEGDYR
                     IKLRNLVRFLEEGDKAKVTLRYRGREMAHQELGVRLIERVKTDLEPYSTIEQYPKMEG
                     RQLIMVLAPKKSGKPSQKTAAKAAVQKKTAASAKAVAPKVAAEAGTGITKK"
     gene            11727..11921
                     /gene="rpmI"
                     /locus_tag="AC16CX_02785"
     CDS             11727..11921
                     /gene="rpmI"
                     /locus_tag="AC16CX_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR3011467.1"
                     /translation="MPKLKTKRGAAKRFRKRAGGSIKRSQAFLRHILTKKNTKRKRHL
                     RGSTGVHGADMSAVRVMLPY"
     gene            11943..12302
                     /gene="rplT"
                     /locus_tag="AC16CX_02790"
     CDS             11943..12302
                     /gene="rplT"
                     /locus_tag="AC16CX_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3011468.1"
                     /translation="MPRVKRGVIARAAHKKVLQRAKGYRGRRKNVFRIANEAVMRAGQ
                     YAYRDRRDRKGEFRALWIARINAAAREHGMTYSRFMNGLKKAAIAVDRKVLADIAVLD
                     KPAFSKFVEKAKASLAS"
     gene            12410..13396
                     /gene="pheS"
                     /locus_tag="AC16CX_02795"
     CDS             12410..13396
                     /gene="pheS"
                     /locus_tag="AC16CX_02795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3011469.1"
                     /translation="MQDVDAIVKEALETFAGIEDAGRLEQAKARYLGKSGALTRLLKG
                     LSKLPAAERPAFGSRVNAAKEKLEAALEQQRDRIQARRLEARLTEESLDVTLPGRGPG
                     IGGIHPVMRTMERIEMLFRSMGFEVADGPEIETDYYNFTALNQPENHPARSMHDTFYL
                     ADGKHLLRTHTSPIQIRYLERHAPPVKIIAPGRVYRHDYDATHSPMFHQVEALWVDEH
                     IAFSDLKGVLIEFLRKFFERNDLRVRFRPSFFPFTEPSAEIDMSFGDDWLEIAGAGMV
                     HPNVLRNVGIDSEKFQGFAFGLGPDRLTMLRYGVNDLRLFFENDLRFLKQFN"
     gene            13491..15845
                     /gene="pheT"
                     /locus_tag="AC16CX_02800"
     CDS             13491..15845
                     /gene="pheT"
                     /locus_tag="AC16CX_02800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3011470.1"
                     /translation="MKFSENWLRTFVNPPLATRELAEAVTMSGVEVEAIEPVAPPFDG
                     VVVGEVLKVGRHPDADRLTVCQVNAGGAPVTIVCGAPDVAAGIKVPVALPGAALPGAS
                     IKVAMVRGVESHGMLCSAKELGLAESASGILILPPEAPIGSDVRKLLELDDRVLTLKP
                     TPNRGDCLSLLGIAREVSAVTGASLALVESRPARATITDRLNVTLEAPQACPRYCGRL
                     VKGVNAGAATPEWMTRRLARSGIRPVSALVDITNYVMLELGQPLHAFDAAKLEGGIRV
                     RYAKAGEKLTLLNGTAPPLTPAFLVIADEAKAVALAGIMGGLATAVSETTRDVFLESA
                     FFDPEAIAGKTRLLNFASDSSYRFERGVDFAGTLAALERATQLVLEVCGGSAGPVTEA
                     RAPLPARDPVRLRLARVERLLGIKLDGIQVGEILRRLRFEFSAAEGAFRVTPPSYRFD
                     LALEEDLVEELARIHGYDRIPATMPAAPATMLPAPESRRDLAAIRRLLAGRDYQEIVS
                     YSFVDAAWEGDFAGNTGPVALANPIASHLSVMRSTLAGGLIHCLAHNLGHKQSRVRLF
                     EVGRCFMTEASGAYRQPPRIGALAYGDARHEQWGLPARRVDFYDVKGDVEALLAPRAA
                     EFIPAQHPALHPGKSASIVIEGRTAGWIGELHPRWQQKYDLPLPPVLFELECEVIVER
                     GLPAYNETSKFPPVRRDIAAEVDEAISYQAILEGLRRERPAIVTEIGLFDIYRGTGVE
                     EGKKSLAFRVLLQDTRKTLTEAEVESAISQLRRTLQQQFNAKLR"
     gene            15857..16159
                     /locus_tag="AC16CX_02805"
     CDS             15857..16159
                     /locus_tag="AC16CX_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR3011471.1"
                     /translation="MTLTKAELADLLFEKVGFNKREAKDMVESFFEEVRTALENGQGV
                     KLSGFGNFQLRDKPQRPGRNPKTGEEIPITARRVVTFHASQKLKAMVEQNYHGSKS"
     gene            16143..16511
                     /locus_tag="AC16CX_02810"
     CDS             16143..16511
                     /locus_tag="AC16CX_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3011472.1"
                     /translation="MEASHNPRTQLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFT
                     QLKPVKRRGNRRYYQHHEVLLIRRIRELLYDQGFTISGARNRLDELVSEPSKAARPSP
                     RSHLAFLRKEIKSVLEVLRG"
     gene            complement(16718..19069)
                     /locus_tag="AC16CX_02815"
     CDS             complement(16718..19069)
                     /locus_tag="AC16CX_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3011473.1"
                     /translation="MASNTETSRLGPGTLPAERTPTKVVRLKPRGQERSRANQGRRLR
                     FTELLLSVSQKMAAIDSLDEVLTTLVELTTAELNAERGSLFLNDPATSELYSRVAQGN
                     IHREIRLLNNSGIAGHVYTTGEGMIIDDAYADPRFNRTIDEQTGFVTKSILCVPIRTI
                     KGEIIGVAQALNKNKGRFTRGDLKVLEAMTSQGTLALQSAQFIERMKAIRKQELEFLD
                     IVSEVTSDIKLGSLLQKVMGEATRMLNAERSTLFLNDEKTNELWSEVGQGLEAQQIRF
                     PNHVGIAGAVFTSSKTINIPYAYADLRFNPAFDKKTGFFTRSILCVPVVNQKGKIIGV
                     TQVLNKRGGPFTNEDESRLRAFTAQVSIALENAKLFSDVQTMKNYNESMLESMSSGVV
                     TLDDAGKIITCNAAGLRILRVTDAGVIDKPAAEFFTGPNAWVVEKLKRVEETQAAEIT
                     MDAEFEFGGENISVNVTLLPLLSIERKRLGSMLMFEDISSEKRMKSTMARYMDPSIAD
                     RLLSTGAEILGGKSVPATVLFSDIRGFTTLTEQLGPQATVSLLNEYFEVMVECIQREG
                     GMLDKFIGDAIMAAFGIPVAHDDDEDRAVRAAIAMIRNLNSWNQERTSEGKLPVNIGI
                     GLNTDVVVSGNIGSKKRMDFTIIGDGVNLASRLEGACKQYSTRILLSEFTFKKLRGTY
                     RAREVDAVVVKGKTQPVAIYEILDYHSEDTFPNLMDAVNHFRDGLAKYRQGKWDPAIG
                     AFRETLRLNPKDKLAEIYVQRCEHLKAHPPGNDWDGVWVMTEK"
     gene            complement(19083..>19414)
                     /locus_tag="AC16CX_02820"
     CDS             complement(19083..>19414)
                     /locus_tag="AC16CX_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011474.1"
                     /translation="RSVQSAVAEAHNEIAQLKATAAALRDSLEQARIDREKAVQAAVA
                     AGHDEINQLKAASSALREALEQARIDKESRIQAAAAAANDEIRQLKDTVAALRAAMER
                     GGPGPAH"
BASE COUNT         3425 a         6129 c         6426 g         3434 t
ORIGIN      
        1 acttcctcgt agacggcctc atcctgcagc acctgctgga aaagaaagtg cggcgccgag
       61 cgcttgatga ggcggggcac gacgatgccg agcgcgagag tggcaagacc ggtcaggatc
      121 cagccgatca aagccagggc gacgccgaca ccgagcaccg gcatcaccac gaatggcagc
      181 agcaggcgcg ccgagaggaa acgcgccgcg ccctgcgggt ccacctcgat cttgacttct
      241 ccgcgcgcat aagcggcggt gaattcggcg tgggtcatac ccaccctaca agtttttcca
      301 gggaccgtct gccttccggc gtcgccatga cgacgagcgt gctgccggcc cgcaccctca
      361 ggtcgccgga cggattgaaa ttccacgact ggtggtcgcg caccgcgagc aggatgtaat
      421 ccgggctctt gagattgagc gagccgaccg tggtttcggg gaacccggcg gggacgatga
      481 tctcctccac ccgcaggttc ccctcggatt tgagcatctc gtccagaaag gtcaccacgt
      541 gcgggcgtat catcgccgag gcgatgcgca agccgccggt aaagtcgggg gagacgatgg
      601 cgtcggcccc ggccttgcgg atcttctcga tgttgcgcac ttcgtggcag cgcgccacca
      661 cccgcacctc cgggttgagc tgcttggcgg tgatggtgat gagcaggttc ttgctgtcgt
      721 caccggtaac cgcgaacacg ccgaccgcgc tttcgatgtg cgctcggatc agcacgtcgt
      781 cgtcgcaccc gtcgccgtgg atgtacatcg tgtcggggtg gtgctcgatg tgcgtctcga
      841 tgctctgcat ggcctcgtcg atcacgacat aggggcggtg cgtggccacc agctcgttgg
      901 cgacgttgcg gccgacgcgc ccgatgccgc acatgatgta gtggtccttc agctcgtgga
      961 tatgcttttc catgcgtcgt ctccatagcg cttcgttgat cttgccctcc accaggtagg
     1021 aaaccatcaa tgacatgatg taccaggtgt tggcgatgcc ggcgaaaccg atcgccatcg
     1081 cgaaaacgcg gcccagcggg ttggatgaaa ggtcgacgac ctcgccgtag ccgatggtgg
     1141 cgatggtgat gaaggtcatg tagaggcagt cgagccagct gtactgcggg ccggcaagca
     1201 cgcgaaagcc cacggtgccg acgacgtgca ggagcgcaag cagtgcgagc gcccggccaa
     1261 agcgccacgc gatctgggca ggggtctcgg cgggaagctg caagggcatg gcgctccgtt
     1321 cgcgcgctat tcgtcgctct agtattcgac cggcagcgga tccaggctcc gccacaggta
     1381 ccacgtcgcc accgagcgcc acggcgccca cgccgcgccg attttgttca tccgcgcgac
     1441 ggacaggggg cggccccgat tgtaatgcag tctcatcgct ctctggagcc ccaggtccgc
     1501 aagcggcagc acgtcagggc gggtgagatg aaaaatgaga aacatttcgg ctgtccagcg
     1561 cccgatgccc ttgaccaggg tcagctcgcc gatcagggcc tcgtcgtcga gagcgtgcca
     1621 gcgtgaggcg tcgagcgttc tgtcgaggaa cttcgccgcg aggtcttgca ggtaggcggt
     1681 tttctgcccg gacagaccgc acgtccgcag cgtcgtcttc cgtgcgctac gaaccgttgc
     1741 gggcgacatc ggcccaagcc gcgcggtgag cctgtcccat acgctctgag cggccttgac
     1801 cgaaatctgc tggccgacga tggagcgcgc aagcgtggag aaggcgtcac cgcgactgcc
     1861 gagcgcgagt ccctggaagc gctcgatcag gccgcgcagc accgcgtcgc gctccgcgag
     1921 ctcgcgcgtc gcctgcggcc agtaatcggg cgttctcatg aggcgcgttg cgcgcggcgg
     1981 cgccggcggc tctcccaggc gcagcgcacg tagagccgcc acgcccagtc cacgacgaaa
     2041 tagccggtta tcgcgagcag cagcgcgagg agaggaagcc cgatcaccag cggcttgccg
     2101 acacttgcga gccaatgcag gaccgccggt atccattcgc tgatgccttt gtcttccagc
     2161 ccgaaagcga gcggcggcaa tccgttgccg ccctccatca gtgcgaaccg gccgagctcg
     2221 aatgcgacga agtagagcgg cacgatggta aaggggttgg agtagagcgt caccagcacc
     2281 gcgatcggca ggttgacgcg gccggcgatc gccagcaagg cggcggccgt gaactggacc
     2341 ggattgctgc cgggaataag gcccgcaaac aggccgaccg ccacgccgcc cgccaccgac
     2401 ctgcggtgca ggtgccacag gttgtggtgc tgcagccacg gtccgagcct ggcaatgtgg
     2461 cggttgctcc tgacggattc gtggctcggc aggaatctgc ggaagaactt gcgcggcatg
     2521 cgtgattggc cgcgcgcgcc gcgcggccgg tgtcattgta accccgtgct tcgcgatacg
     2581 agatacggga tacgaggcac gagatgcgcg cctctttatc aacctcgaat ctcgcatccc
     2641 gtatcccaca tctcgcatcc cgcaccccgt ttctcggatc accggtcacc gctcacgcct
     2701 cctgggccgt ccacccgctg tccgcctcgc gcgttacacg acaatgcggc tcaacatcgt
     2761 ctgtttcgtg gcgggcgcgt ggtggctgca gcagcaggca gcgctgcccg ataccggctg
     2821 gacgtgggtg ctggccgcgg ctggcctcgc cacaacggtc gtgcgcttcg aaagcgtgac
     2881 actgcgcctc gcgtgcggtc tatgtgtgaa ggcggcgtgt ttcgcgctgg gcttttcctg
     2941 ggcggcatgg tgcgcgcagc aaaggctggc cgatgccttg ccggcggaat gggaggggcg
     3001 tgatatcgcc gtcatcggcg tagtggccgg cctgccgcag acgtacgagc gcggggtgcg
     3061 cttcgagttc gacatcgagc gggtgctgac tccgcaggcg cgcgtgcccg agcacatcat
     3121 cctgtcgtgg tggggcagcc cggcgcgtaa tgacaggccc gccacttttc cgccgctcaa
     3181 cgcgggagag cgctggcagc tgatggcccg gctcaagcgg ccgcacggca ccgccaatcc
     3241 gcacggattc gactacgagg cgtggctgct cgagctcaac ctccgcgcaa cgggctacgt
     3301 gcggccgaag gggggcagcc accggctggc cagtatggtg catgaaccga aatactggat
     3361 cgaacgcgcg cggggcatga tccgcgcacg catccaggcc gcgctgccgg acgcgccgta
     3421 cgtcggcgtc atcgccgcgc tcgcgatcgg ggaccaacgc gcgattccgc cggaacagtg
     3481 gcagaccttc acccgcaccg gtgtcaacca tctgatgagc atctcgggcc tgcacgtcac
     3541 catggtttcg ggtctcgtgt ttgccctcgt ttatggcctg tggcggcgcg ttccacggct
     3601 cgtgctggtg ttgccggcgc tcaaggccgc cgcgctggca gggcttggcg cagcgctgct
     3661 ctacgcggct ctcgccggct tcgccgttcc ggcccagcgc acggtctaca tgctggccgt
     3721 ggtggcagcc gcgctgtggc tgggcgttat cgagacggcg tctgtcgtgc tgtgcgcggc
     3781 gctcctcgtc gtcgtgctgc tcgatccctg ggcggtgctt gcaccgggtt tctggctctc
     3841 gttcggggcc gtcgccgtga tcatgtacgt ctcggtcggt cgcatcggcc ggacgcgctg
     3901 gctcgccgac tgggcgaggg tgcagatcgc cgtgacgctg gcgctgattc cgccgctgct
     3961 ggcgctgttc cagctggttt cgatcgtctc gccgctcgcc aacgccgtcg cgatccccgt
     4021 cgtgagcctg gttgtcgcgc cgctggcgct gatcggggcc gcgatgccgt tcgatttcgt
     4081 cctgcatata gcgcatctcg tgatgagcgg atgcatggcg ttcctcgagt ggctgagcgc
     4141 gcttcccgac gcggtgtggc agcagcacgc acccccgtca tggtcggtga tcgtcgccgt
     4201 cgcggccgtg gcctggctgc tcgccccgcg cggcctgccg gcacgctggc tgggcgtggt
     4261 cgggctgctg ccgctcttcg cgaccgcgcc gggcgcactc aacgcgggag acgtcgagat
     4321 catcgtgctc gacgtggggc agggcgtatc cgcgctggtc cgcaccgcga atcacgcgct
     4381 gctctacgat gcggggccgc agtttggtcc cggcgccgac agcggcagcc gcatcatcgt
     4441 gccgtatctg cgcgcggccg gcgtgaagcg gctcgacggt tttgtggtga gccatgacca
     4501 cgatgaccac tggggaggcg ccgcctcggt gctgcaagcg gtgcccgtgg cgtgggtgct
     4561 gacctcgctg cccgacctcg atccgctggt ggtccaggcg gagccggctt tgcgctgcga
     4621 ggcgggccag cggtgggaat gggataacgt gcgtttcgag gtgctgcatc cggcaagcgc
     4681 cagctacgac gatcccgtgg tcaaggacaa cgaccgcagt tgcgtgctcc gagtggaggc
     4741 gccgggcggg cgggcgctgc ttcccgccga tatcgaacgc cgttccgagg aggagctgct
     4801 gcagcgcgaa cgcgaccgat tgcatgtcga tgtgctgctc gcgccgcacc agggcagccg
     4861 gacttcttcg accccggctt tcgtgcaggc ggtcagcccg cggatcgtgg tgttcccggt
     4921 gggctatcgc aatcgttttg gccatcccca ccgcgaggtc gtggagcgtt accggaatat
     4981 cgggacgcgc gtctaccgca cggatcgcga cggcgcggta accatctcga tccgcccgga
     5041 agggacgatc agcgtgacct cctatcgcgc gttttatcgg cggtattggc aaaccgtttt
     5101 ggagggagat ccggcgcccg atccggaaga gttctgatca acggcgcgcg tctacgtcgt
     5161 agggatgcgg atcatcggct ccaggcaacc gttgtgccaa actcctgtcg aatccctgcg
     5221 gaccgattga tcagaccgcc tgctgcgcct gcagacggtt gcccgggcgt gctcgttgca
     5281 atgctgatgg acgccataaa gtataatgaa tacaagtaga tggctaagat tcctcaacta
     5341 aagttagtgc acccgccgac gcccgatttt tcgacggggt tcagcgagca tgccgcacgg
     5401 cttgcgcccg ccgaccggga ccgtgttcac gccgccttag ccgtggcgga acccctgctt
     5461 ggggaggcgc gtttgccggg cggggaagtt gccctgaacc atgcgctggg aaccgcggcc
     5521 atcgtctccg agctgaagct cgacggcgac gcggtcgccg ccgcgctcct tacgcccctt
     5581 gccgtgatcg cccccgaacg catgcgcacg ctgaaggaac gttgcggcac cgtggttgcc
     5641 gagctcgtcg agggcgttgc acgcatggcg caaattcacg ccctctcgag ccgtggcgcg
     5701 cccgcggcga ggccggcagg ccaggaagcc cagctcgagg cgctgcgcaa gatgctgctc
     5761 gccatggtgc aggatatccg ggtggtgctg gtgaagctcg ccgaccacac ccaggaactg
     5821 cgctttgcgg ccaagtccga cgacgaggaa gcaaagcgcg ggaccgctgc tctcacgacc
     5881 gacatcttcg ccccgctcgc caaccggctg ggggtatggc agctcaagtg ggaaatggaa
     5941 gacctcgcat tccgcatcct cgagccggag acctacaaga agatcgcgcg gatgctcgat
     6001 gagaaacgcg tggaccggga gcgttatata gagaatgtca ccgccctgct gaaaggcgaa
     6061 ctcgcacgcg ccggtatcgc cgcggaggtt acagggcggc ccaagcacat cttcagtatt
     6121 tacaaaaaga tgaagataaa agcagttgat tttgaatcac tttatgacgt gagagcggtg
     6181 cgcgttctgg tccaggacgt cacggactgc tacaccgcgc tgggtctcgt tcacaatctg
     6241 tgggcgccga ttcccgggga gttcgacgac tacatcgcga agcccaagag caacctgtac
     6301 cgctcactcc acaccgccgt gatcgggccg gaggacaagg cactggaagt ccagatccgc
     6361 actcacgaaa tgcaccagca ctctgagctc ggagtcgccg cgcattggcg ctacaaggag
     6421 gactcgcgcc gcgaccggga ttacgacagg aagatcgcct ggctgcggca gattctggag
     6481 tggaagggcg aggctgggga cgccggcgag ctggcggaac gtttccgtac gggcctgttc
     6541 gacgacacga tctacgtgct gacgccgcag ggcagggtga tcgcgctccc gaaaggcgcc
     6601 acgccggtgg acttcgccta tcacgtgcac accgagctgg ggcaccgctg ccgcggcgcc
     6661 aaggtggacg ggatgatggt gccgctcaac acgccgctcg tgaacggcca gcgggtcgag
     6721 atccttgccg cgaaacaggg cgggccgagc cgggactggc tcactccggc gctgggttat
     6781 gtgatgagtc aaacagcccg cgtcaagatc cggcagtggt acaaccgcca gaaccacgaa
     6841 gcggcggcgg cccagggtcg cgcggtggtg gaaaaggagc tgcagagaca gggaatgacc
     6901 ggcctcaacc tcgaacatct cgcggcaaag ctcggctacg gccggctcga tgattttctg
     6961 gccgtggtcg gacgcggcga aatcggtccg cgccagctgg agcaggcgat gcacgcgctt
     7021 aatccgcaca cgggtgtcct gaagacagaa gcgcgggacg aagcgccggt ggcgccggca
     7081 cggcgggaat cgcccgctgc gcgcaaaggc ggcgtgctgg tcgtgggtgt ggatcgattg
     7141 ctgacggttc cggcgaaatg ctgcaagccg gccccgcccg acgtcatcgt cggcttcatt
     7201 acacgcggac gcggcgtcac catccaccgt gcggcctgcg ccagcctgaa tcggctcgat
     7261 cccgagcgga gggtggctgc cgagtggggc gaagccgaag acgcgacttt cccggtcgac
     7321 atcgaggtcg acgccgtgaa gcgcgcccgg cagcagcgcg aaatctcgga aatgctcgcg
     7381 cgcgaaaaaa tccgtatcgt cggttcgcgc tccgtggaag tcgatgccgc ggtgcgcctg
     7441 cgctacaccc tcgaggttgc caaccttgca cagctctcgc gcgtgctcgc gctgatccgg
     7501 gaagtgcgcg gcgtcatgcg cgccgtgcgg cgctgacgcg cggtgatgag tgatgggtga
     7561 taagtgatgt ggactgctcg gcttcgcctc gtcactccac ctattcggtg aactggatcg
     7621 ttaggcccgt tacgccgggc agaccctcgt agcgcgacga ccattcctcg ccgggccgaa
     7681 tcggcatcgc tgcggtgagc gtgccggtgg tgatcacttc tcccgccgcg agcggctcga
     7741 accgcagctg acgcgaaagc acgtccgcga ggtgcgcgag cgcggcggcg ggatgtccca
     7801 gcgcatttgc gccgacgccc tggtccatca ccctgtcgct cctgctcagc gtaatccggc
     7861 agtcgcgcaa ctgcgaggcg agtgcaggcg ggtcagcgct gcggatatca cgcggcgcgc
     7921 cgacgaccag ccgccagtga aacgaaaagt ccgcggccga gtcagcgggc ttgaatttcc
     7981 agtcggcgaa gtggcaatcc acgatctcga agctcggggc cagccattcg accgcttcca
     8041 ggatttgcgc gggatcatcc acgccggcag gcaggggggc cttgagccgg aaagcgatct
     8101 ccggttcgag gcgcggccgc acactgccct tgagcgaaac ggtggcgcgg ttgccggagg
     8161 cacgcaacag cgtccggtcg taaacgtgcg cccagatcgg ggcggtcgcg ccgtactcgg
     8221 cccagatgtt gcggttggtg aagccgatct tgcggccgac ggtgcgctcg ccgcgcgcgc
     8281 gccgcagctt caagatttca gccgctacgg catacccttc atcccagccg aataccggat
     8341 agcgttccgc gatggacgga atggttccgc cgctatcgag cgcgtcgagc agctcgcgcg
     8401 caatcttcct cgcgtcaatc ttggctttca tcggtgtagc tcggcgtttg cgttctactt
     8461 cgcgcagcgc ggcgccacgt catgcgacgc cttccaccgc caacatgcgc gtcttcgccg
     8521 cgcggtcgcg gatctcgcag gctttcccgt attcgggcga tgaccgccat cgcttcgcct
     8581 gctgcatgcc cggaaattcg atcacgacga tgcgctgcgg gttccagtcg ccgtcgaaga
     8641 cttctgtttt gccgccgcgc accaggaatt tcccaccata agcgacgacg gaggcacccg
     8701 ccagcttgcg gtagtcctcg atccagaccg cgtcggtcac ggtcatttcc acgatcacgt
     8761 atgcggccat cgtatccccc gggtttcgtc gcccaagccc atgattttat ttgaagctac
     8821 tacctgattc cgctacaatt cgcactcctt gaggcgcgta gctcagatgg ctagagcgca
     8881 tccttgacat ggatgaggtc gttggttcga ttccaatcgc gcctaccaga tgttaaaacg
     8941 gacgcgcagc accgttttaa ctctgcgtgt tggaacggac gccgtgcgcc gttcaacacg
     9001 cagggtacaa aatagacgag tcagaggcgg cggtcgcaac ggccgtttct ggctcttgtt
     9061 tttttggacg atagcatgcc ggaaatacgt ttgcccgacg gatcggtgaa gaaattcgac
     9121 ggggcagtga ccgtggccga gctcgctcag gcgatcgggc cgggcctcgc gcgcgcggcg
     9181 ttggcgggaa aggtcaacgg caagctggtt gatacgtctt tccgcatcga ctccgatgcg
     9241 gaagtcgcga tcatcacgga caaggaccgc gaagggctgg agatcctgcg gcattccacg
     9301 gcgcatcttc ttgcccatgc ggtcaaggag ctgttcccgg atgcccaggt caccatcggg
     9361 ccggtgatcg aggacggctt ctattacgat tttgcctata aacggccgtt tactccggag
     9421 gaccttgctg cgatcgagaa acgcatgcag gagatcacga agcgcgacat caaggtcgtg
     9481 cgcgaggtga tgccgcgtga tgcagcggtg aagttcttcc gcgaccaggg cgagcgctac
     9541 aaggccgaga tcatcgcggc gattcccgac ggcgaggcga tttccctcta ccgccaggac
     9601 aacttcgtgg atctctgccg cggcccgcac gtgccttcca ccgggaagct cagggtgttc
     9661 aagctgatga agctagcggg cgcctattgg cgcggcgatt cgaagaacga aatgctgcag
     9721 cggatctacg gcaccgcgtg ggcgaagaag gaggaccagg acatctacct ccaccggctg
     9781 gcggaggccg agaagcgcga ccaccgcagg ctgggacgga gcctcgagct gttccacacc
     9841 caggacgaag cgccgggcat ggtgttctgg catcccaagg gcacggtgat ctggcagcaa
     9901 atcgagcagt acatgcgacg gctgctttcc gaaaacggct atcaggaggt gaggacgccg
     9961 caggtgctcg acgtttcgct atggaagcgc tcggggcact gggacaactt ccgggagaac
    10021 atgttcttca ccgagtcgga gtcgcgcgac tacgcggtga agccgatgaa ctgcccggga
    10081 cacgtccaga tattcaacca ggggctgaag agctaccgcg acctgccgtt gcggctcgcc
    10141 gagttcgggt cctgccaccg caacgagcct tccggcgcgc tgcacgggct gatgcgggcg
    10201 cgcagcttca tccaggacga tgcgcacatc ttttgcaccg aagcgcagat cgaacccgaa
    10261 gtgatccgct tcatcgagct gctgcagcgg gtctatgccg acttcggatt cgacgagatc
    10321 ctggtcaagc tgtccacccg gccggcgaag cgcgtcggca ccgacgcgca atgggacaag
    10381 gccgaagcgg cgctcaagac cgcgctcgac cacaaggggc tgggttacga cctgcagccg
    10441 ggcgagggtg cgttttatgg ccccaagatc gagttctcat tgaaggacag cctgggccgc
    10501 gtgtggcagt gcggcaccat gcagctcgat ttctcgatgc cggagcggct cggcgcggag
    10561 tacgtggcgg aggacaacgc gcgccgcgtg ccggtgatgc tccatcgggc gatcctcggc
    10621 tccttcgaac gcttcatcgg catcctgatc gagcattacg cgggggcgat gccggtctgg
    10681 ctggcgccgg tgcaagtggt ggtgatgaac atcaccgagc accaggcgga ctatgccgaa
    10741 cgcgtcgtga gcgaattgag acgggccggg ttgcgcgtcg ccgccgactt gaggaacgag
    10801 aaaatttcct ataaaattcg ggaacatagt ttgcagaagc tgccctacca agtcatcgtc
    10861 ggtgacaagg aaaaggcggt gcaacaggtt gccgtgcgca cccgcagggg cgaggatctc
    10921 ggccagatga gtctggccga ttttgtctcc aggctccggg cggaagaggc ccggttggga
    10981 cgcgcggcgc aggattaacg ggacgcaggg ggttctacat cgcacaggaa aaggaaccgc
    11041 ggatcaacgg cgagattacc gcgccggaaa tccgggtgat aggcgccaac ggcgagcaga
    11101 tcggcatcct gagcctggcc gccgccaaca agctcgcgga ggaagccgag ctcgatctcg
    11161 tcgagatcgc tccgatggcg acaccgccgg tgtgtcgcat catggattac gggaagtaca
    11221 agtaccgtga gagcaagaag cggcacgagg ccaagctcaa gcagaagcag atcgtcgtca
    11281 aggaaatcaa gttccggccc ggcaccgacg agggcgacta tcggatcaag ctgcggaacc
    11341 tggtccggtt cctcgaggag ggcgataagg ccaaggtgac gctgcgctac cgcggccgcg
    11401 agatggccca ccaggagctc ggcgtgcgcc tgatcgagcg ggtaaagacc gacctcgagc
    11461 cgtacagcac gatcgagcag tacccgaaga tggaaggccg ccagctgatc atggtgctgg
    11521 cgccgaagaa gagcgggaag ccttcccaga agactgccgc gaaagcggcg gtacagaaga
    11581 agacggcggc ttccgcgaaa gcggtcgccc cgaaggtcgc ggcggaagcc gggaccggca
    11641 tcacgaagaa gtagttgtcc gggttcccga agccctgctt cctggcaggc acccggcgct
    11701 aggctaaaac agagatagga gttgtcatgc ccaagctgaa aaccaaacgc ggcgccgcca
    11761 agcgcttcag gaagcgcgcc ggtggcagca tcaagcgcag ccaggcgttc ctgcgccaca
    11821 tcctcaccaa gaagaacacc aagcgcaagc gtcacctgcg cggcagtacc ggggttcacg
    11881 gggccgacat gagcgcggtc cgcgtgatgc tgccgtactg agagcctcca agggagaccg
    11941 ccatgcctcg agtcaaacgc ggcgtcatcg cccgcgccgc ccacaagaag gttctccagc
    12001 gcgccaaggg ttaccgcggc cgccgcaaga acgtgttccg catcgccaac gaagcggtga
    12061 tgcgggccgg ccagtacgct tatcgcgacc gccgcgaccg caagggcgag tttcgcgcgc
    12121 tgtggatcgc gcgcatcaac gctgcggcga gggagcacgg gatgacctac agccggttca
    12181 tgaacggcct gaagaaggcg gcgatcgctg tggaccgcaa ggtgctggcc gacatcgcgg
    12241 tcctcgacaa gccggcgttc tccaagttcg tcgagaaggc caaggccagc ctcgccagct
    12301 gaagtgcaaa gcggggctcg gggcttgggg ctcgaggctc gctcaagcaa agctgacaag
    12361 cgggtttctc cagcccccag cccctagtcc cgagtcccga atcttgggca tgcaagacgt
    12421 tgatgccatc gtgaaggaag cgctcgagac gttcgcgggc atcgaggacg cgggccggct
    12481 cgagcaggcc aaggcgcgct accttggcaa atccggcgcg ctgacccggc ttctgaaagg
    12541 gctctccaag ctgcccgctg ccgagcgccc cgcgttcggc agccgcgtca acgccgccaa
    12601 ggagaagctc gaggcggcgc tcgagcagca gcgcgaccgc atccaggccc gccggctgga
    12661 ggcacgcctg acggaggaat cgctcgacgt gacgctgccc ggccgcgggc cgggaatcgg
    12721 gggcatccat cccgtgatgc gcaccatgga gcgcatcgag atgttgttcc gctcgatggg
    12781 gttcgaagtg gccgacggtc cggagatcga aaccgattac tacaatttca ccgcgctcaa
    12841 ccagcccgag aaccacccgg cgcgctcgat gcacgatacc ttctatctcg ccgacggcaa
    12901 gcacctgctg cgcacccaca cctcgccgat ccagatccgc tacctggagc gccacgcgcc
    12961 cccggtgaag atcatcgcgc cgggacgcgt gtaccggcac gactatgacg ccacgcactc
    13021 gccgatgttc catcaggtcg aagcgctgtg ggtggacgag cacatcgcct tttccgatct
    13081 caagggcgtg ctgatcgagt ttctacgcaa gttcttcgag cggaacgatc tcagggtccg
    13141 cttccggccc tcgttttttc ccttcaccga accctccgcc gagatcgaca tgagttttgg
    13201 cgacgactgg ctcgagatcg cgggcgccgg aatggtgcat cccaacgttc tcaggaacgt
    13261 cggcatcgac agcgagaaat ttcagggctt cgctttcggg ctgggccccg accgcctcac
    13321 gatgctgcgc tacggtgtga acgacttacg gctgttcttc gagaacgatt tgaggttttt
    13381 gaaacaattc aattagctac agagagcgca gagaacacag agaatggtga tcaacgggcc
    13441 tgttttccga ttctctctgt gatctctgtg ttctctgtgg ccaagatgtt atgaagttct
    13501 ccgaaaactg gctccgcacc ttcgtcaatc cgccgctcgc gacgcgcgag ctggcggagg
    13561 ccgtcaccat gagcggggtc gaggtggaag cgatcgagcc agtcgccccg ccgttcgatg
    13621 gagtcgtggt gggcgaggtg ctcaaggtcg gacgccaccc cgatgctgac cggctgacgg
    13681 tatgccaggt caatgccggc ggcgcgcccg tgaccatcgt gtgcggcgcc ccggatgtgg
    13741 ccgccggcat caaggttccc gttgcgctcc ccggagcggc cttgccgggc gcttcgatca
    13801 aggtggcgat ggtgcggggc gtggaatcgc acggcatgtt gtgctcggcg aaggaactcg
    13861 gcctagccga gagcgcatcg ggcatcctga tattgcctcc cgaagcgccg atcggatcgg
    13921 atgtgcgcaa gctcctcgag ctggacgacc gggtgctgac gctcaagccc acgcccaacc
    13981 gcggcgattg cctgagtctg ctcgggatcg ccagggaagt ttcggccgtg accggcgctt
    14041 cgcttgccct ggtcgaatcc aggccggcgc gcgcaaccat caccgatcgc ctgaacgtca
    14101 ccctggaggc gccgcaggcc tgcccccgct actgcgggcg gctggtgaaa ggcgtcaacg
    14161 ccggagcggc gacgccggaa tggatgacgc gccggctcgc gcgcagtggc atccgcccgg
    14221 tcagcgcact ggtggatatc accaattacg tcatgctcga gctgggccag ccattgcatg
    14281 cattcgacgc cgcgaagctc gaaggcggca tccgggtgcg ctacgcgaag gcgggggaga
    14341 agttgacgct tctcaatgga accgcgcccc cgctcacgcc cgcgttcctg gtcatcgccg
    14401 acgaggcaaa agctgtggcg ctggcgggca tcatgggcgg gcttgccacc gcggtgagcg
    14461 agacgacgcg tgacgtgttc ctcgagagcg cgttcttcga tccggaggcg atcgcgggga
    14521 aaacccgctt gctcaatttc gcctcggatt cttcctaccg cttcgagcgc ggcgtcgatt
    14581 tcgcaggaac gctggcggcg ctcgaacgcg ccacgcagct tgtcctcgag gtctgcgggg
    14641 gctcggcggg accggtcacg gaggcgcgcg cgccgctgcc cgcgcgcgat ccggtgcggc
    14701 tgaggctggc gcgcgtggag cggctgctcg gcatcaagct cgacggcatc caggtgggcg
    14761 aaatcctgcg ccggctgcgt ttcgagttct ccgcggccga gggcgcgttc cgggtgacgc
    14821 cgccgagcta ccgcttcgac ctcgcgctcg aggaggatct cgtcgaggag ctggcccgca
    14881 tccatggcta cgacaggata ccggctacca tgcctgcggc accggccacc atgctgccgg
    14941 cgcccgaatc gaggcgggac ctcgctgcaa ttcgcaggtt gctcgcaggg cgtgactacc
    15001 aggaaatcgt gagctacagc ttcgtggacg cggcctggga aggcgatttc gccggcaata
    15061 ccggaccggt cgctctcgcc aacccgatcg cgagccacct ctccgtgatg cgctcgacgc
    15121 tggccggcgg gctgatccac tgccttgctc acaacctggg ccacaagcag agccgggtac
    15181 ggctgttcga agtcggccgc tgcttcatga cggaggcatc gggggcgtac cgccagccgc
    15241 cacggatcgg ggcgctcgcc tacggggatg cgcgccacga gcagtggggt ctgccggcgc
    15301 ggcgcgtgga cttctacgat gtgaagggcg acgtcgaggc gctgctcgcg ccgcgcgccg
    15361 cggaattcat cccggcgcag catcctgcgc tgcatcccgg caaatcggcg agcatcgtga
    15421 tcgagggcag aacggccggc tggatcgggg aattgcaccc gcgctggcaa cagaagtatg
    15481 atctgccgct ccccccggtc ttgttcgagc tcgaatgcga ggtgatcgtc gagcgggggc
    15541 tgccggctta caacgagacg tcaaaattcc cgccggtgcg gcgcgacatt gccgccgaag
    15601 tggacgaagc gatcagctat caggcgatac tggagggctt gcgccgggaa cggcccgcga
    15661 tcgtgaccga aatcgggctg tttgacatct accgcggaac gggtgtagaa gaaggcaaaa
    15721 agagtcttgc attcagggtg ttattgcagg atactcgcaa gaccttgacc gaagcggaag
    15781 tcgaatccgc catctcgcag ctgcgccgga cactgcagca acaattcaac gcgaaactcc
    15841 gttgaaaggg gacaacatga cgttaaccaa ggccgaactc gcggaccttc tgttcgagaa
    15901 ggtcgggttc aacaagcgcg aagcgaagga catggtggag tcgttcttcg aggaggtgag
    15961 gaccgcgctc gagaacgggc agggcgtgaa gctctccggc ttcggaaact tccagctacg
    16021 cgacaagccg cagcggccgg gcaggaaccc caagacggga gaggagatcc cgatcacggc
    16081 gcgccgcgtc gtgacgttcc acgcgtcgca gaaactgaag gcgatggtgg agcagaacta
    16141 ccatggaagc aagtcataac cccaggacgc agctgccgcc gatccccgcg aagcgctact
    16201 tcaccatcgg cgaggtcagc gagctgtgcg gcgtcaagcc gcacgtgctg cgttactggg
    16261 agcaggaatt cacccagctg aagccggtga agcggcgcgg caaccggcgc tactaccagc
    16321 accacgaagt gctgttgatc cgcaggatca gggaattgct ctacgaccag ggctttacca
    16381 tcagcggcgc gcgcaaccgg ctcgacgaac tggtttccga gccgtccaag gcggcacggc
    16441 cgtcgccccg ctcgcatctc gctttcctgc gcaaggaaat caagagcgtc ctcgaagttt
    16501 tacgcggctg agcgccgcgt aaaacttacc gtactcatcg gggttgattc aggatctgct
    16561 gcggcgttgg cgcttgatcc tgaggcggat cgacccgttg tagtgcgagc ggttacgggc
    16621 aagctaatcg aaagagatcg agaatccggt ggaagccgtt atctaccatt tgttctgacc
    16681 cgctttccca atgaaaaata aacagccggc cggctgttta tttttcggtc atgacccaca
    16741 cgccatccca gtcattgccc ggcggatggg ccttgaggtg ctcgcagcgc tggacgtaga
    16801 tttcggccag cttgtccttc ggattgagcc gcagggtctc gcggaaagcc ccgatcgcag
    16861 ggtcccattt tccctggcgg tacttggcga gcccgtcccg aaagtgattc accgcgtcca
    16921 tcaggttcgg gaacgtgtct tcgctgtggt aatcgaggat ttcgtagatc gcgaccggct
    16981 gggtcttgcc tttgaccacc acggcgtcca cttcgcgcgc gcggtaggtg ccgcgcagct
    17041 tcttgaaggt gaattcgctc aacaggatcc tcgtgctgta ctgcttgcac gcgccctcga
    17101 ggcgcgaggc gaggttgacg ccgtcgccga tgatggtgaa gtccatcctc tttttcgagc
    17161 cgatgttccc ggataccacc acgtcggtgt tgagcccgat gccgatgttc accggcagtt
    17221 tgccttcgga ggtgcgctcc tggttccagc tgttgaggtt gcggatcatg gcgatcgccg
    17281 cgcgcaccgc gcggtcctcg tcgtcgtcgt gcgcgaccgg tatgccgaag gccgccatga
    17341 tggcgtcgcc gatgaacttg tcgagcatgc cgccttcgcg ctggatgcat tcgaccatca
    17401 cctcgaaata ctcgttgagc aggctcaccg tcgcctgcgg tcccagttgc tcggtcaggg
    17461 tcgtgaaacc gcgtatgtcc gagaacagca ccgtggcggg cacgcttttc ccgccgagga
    17521 tctcggcgcc cgtcgagagc aggcgatccg cgatggaggg gtccatgtag cgcgccatgg
    17581 tggacttcat gcgcttctcg ctcgagatgt cctcgaacat cagcatcgag cccaggcgct
    17641 tgcgctcgat gctgagcagc ggaagcaagg tcacgttgac cgaaatattc tcgcccccga
    17701 actcgaactc cgcgtccatc gtgatctccg ccgcttgcgt ttcctccacg cgcttcagct
    17761 tttccaccac ccaggcgttc ggccccgtga agaactcggc cgccggcttg tcgatgacgc
    17821 cggcgtccgt cacgcgcaga atgcgcaggc ccgcggcgtt gcaggtgatg atctttcccg
    17881 cgtcgtccag ggtgaccacg ccgctcgaca tgctctccag catgctctcg ttgtagttct
    17941 tcatggtctg cacgtcggag aacagcttcg cgttctccag cgcgatggac acttgggcgg
    18001 tgaacgccct caaccgcgat tcgtcctcgt tggtgaaggg gccgccgcgc ttgttgagca
    18061 cctgggtcac gccgatgatc ttgcccttct ggttgaccac cggcacgcac aggatcgacc
    18121 gcgtgaaaaa gccggttttc ttgtcgaagg cgggattgaa cctcaagtcg gcgtaggcgt
    18181 acggaatgtt gatggtcttg ctcgaagtaa acacggctcc cgcgatgccg acgtggttgg
    18241 ggaaacggat ctgctgcgcc tcgagcccct ggccgacttc ggaccacagc tcgttggtct
    18301 tttcgtcgtt gaggaagagg gtggagcgct cagcgttgag catccgggtc gcctccccca
    18361 tcactttctg aagcagcgat cccagcttga tgtcggaagt gacttccgag acgatgtcga
    18421 ggaactccag ttcctgcttg cggatcgcct tcatgcgctc gatgaactgg gcgctctgca
    18481 gggcgagcgt gccctggctg gtcatcgcct ccagcacctt gaggtcgccc ctcgtgaacc
    18541 tgcccttatt cttgttgagc gcctgcgcga cgccaatgat ctcgcccttg atggtccgta
    18601 tcggcacaca caggatgctc ttcgtgacga acccggtctg ttcgtcgatg gtgcggttga
    18661 aacgggggtc ggcgtaggcg tcgtcgatga tcatcccctc gccggtcgtg tagacgtggc
    18721 ccgcgatgcc cgagttattg agcaggcgga tctcgcggtg aatattgccc tgggcgacgc
    18781 gcgagtagag ctcactggta gcggggtcgt tgaggaacag cgatccgcgc tcggcgttga
    18841 gctccgcggt agtcaattcg acgagggtgg tgagcacctc gtccagcgaa tcaatcgccg
    18901 ccatcttctg cgatacggac agcagcagtt cggtgaagcg caatcgccgg ccctggttcg
    18961 cacgcgaacg ctcctgcccc ctaggcttga ggcgcaccac cttggtcggc gtcctttccg
    19021 cgggcagcgt tccaggaccc aggcgcgagg tttccgtatt ggaggccata ggggtattgt
    19081 gatcagtgcg cgggtccggg cccgccgcgt tccatggccg cgcgtagtgc agccaccgtg
    19141 tctttaagct gccggatctc gtcgtttgcg gccgcggccg cagcctgaat ccgggattcc
    19201 ttgtcgattc tcgcctgctc gagcgcctcg cgcaacgcgg aggaagccgc cttgagctgg
    19261 ttgatctcat cgtggccagc ggccactgcc gcctgcacgg ctttctcccg gtctatgcgg
    19321 gcctgctcca ggctgtcgcg cagcgccgcg gcggtcgcct tgagctgggc gatctcgttg
    19381 tgcgcctccg caaccgcgct ctggacgctg cgtt
//
LOCUS       DBNGWZ010000093         3897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_763796, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000093 DBNGWZ010000000
VERSION     DBNGWZ010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_763796"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="AC16CX_02825"
     CDS             complement(<1..2304)
                     /locus_tag="AC16CX_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3011475.1"
                     /translation="MDAGDRLVTFTGFGASVKRKEDPALLRGEGQFVDDIHLPGMLHA
                     AFVRSPYAHAKVGRIDKVAALAVPGVHAVLTYADLPESVRRQTLPLLVPHPAIKQPYM
                     PYALAKDEVCYAGEPVACVIAESRYIAEDAAQRVEVDYEPLPAVNDCRTALEPGTPLA
                     HAGAESNVAARFPVKAGDADRAFGAAAHVFRERIHQHRGGPFFMECRGAVARYDAPAQ
                     SYLLYVSSQGSHRLKRCMLDMFDLGDHQVRVVAPDVGGGFGPKGSYYPEYVCVAVAAR
                     LTDRPVKWIEDRRENFLATHQERDQYWDVEIAVDAGAKIIGVRGQLVHETGAYVPWGI
                     VLPWITATTVPGPYVIPNFKLDVVSVFTNKIQTTPVRGAGRPEAVVTMERLMDRVARE
                     LRLDPAEVRRRNFIRPEQMPYNVGIIFRDGRPVTYDSGDYPACQAAALEAADHGGFVA
                     RRDAARKAGRYLGIGISNAVEATGLGPYEGATVRVATSGKIVVYTGATPQGQSHKTTL
                     AQIAADQLGVDYNDVAVVTADTAAISLGIGTFAARTAVNAGSSVHVAAMEVAAKAKKI
                     AAGMMECVEDDLELADGYVRLKSAPDVRKSLREIAVRAIGTPGFSMAGGLPPGLEHTA
                     HFTPDQSVYSNGTHVAEVEVDIETGAVRILRYTVAHDCGRVINPMIVDGQIVGGVAHG
                     IGNALFERMVYDDNAQPVSTNFGEYLLPLATDVPRVDLVHLETPSPLNPLGIKGAGEG
                     GTIPAIAAIVAAVENALAPFGVTIAEAP"
     gene            complement(2280..2765)
                     /locus_tag="AC16CX_02830"
     CDS             complement(2280..2765)
                     /locus_tag="AC16CX_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3011476.1"
                     /translation="MPDTRTIAITVNGEEYTREIDTRLTLVDFLRHELNLTGTHVGCE
                     HGVCGACTVLVGGASVRACLMLAVQCDGEEVATVESLADGDKLNPLQQAFRDHHGLQC
                     GFCTPGILMTLTEFLRDHPEPGEAEVREALSGNICRCTGYQGIVDAALAAAKWMRETG
                     S"
     gene            complement(2758..3633)
                     /locus_tag="AC16CX_02835"
     CDS             complement(2758..3633)
                     /locus_tag="AC16CX_02835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3011477.1"
                     /translation="MKPAPFIYHDPRTAAEACDLLATHENARVLAGGQSLVPMMNFRY
                     VMPDHLIDLNKVGELAYLRCEGDALHVGAMTRQRELEFSEEVGKRCPVLREALDHVGH
                     RQTRNRGTFGGSLCHLDPSAELVNVAALLGGRLHARSKRGTRDIPFAEFAVGYMTTSL
                     RPDELLAGITLPLPARGRGHAFVEFARRHGDFAIVASSALIGLDRSGRIADAAIALSG
                     LSHAPVRPAAVEQALRGEKPGAATFKAAAAAAGKLDATADAYVTAAYRQHLARVLTYR
                     ALEQASARALEMANA"
BASE COUNT          594 a         1374 c         1292 g          637 t
ORIGIN      
        1 cggcgcttcg gcgatggtca cgccgaacgg cgcgagggcg ttctcgaccg cggccacgat
       61 cgcggcaatc gcgggtatgg tgccgccctc gcctgcgccc ttgattccca gcgggttcag
      121 cggcgacggc gtctccagat gcaccaggtc cacccgcggc acgtcggtcg cgagcggaag
      181 caggtactcg ccgaagttgg tcgacaccgg ctgcgcgttg tcgtcgtaca ccatgcgctc
      241 gaacaatgcg ttgccgatgc cgtgcgccac gccgccgacg atctggccgt cgacgatcat
      301 cggattgatc acgcgcccgc aatcgtgcgc gacggtgtag cgcagtatcc ggaccgcgcc
      361 ggtctcgatg tccacctcga cttcggcgac gtgcgtgccg ttcgaataga ccgattggtc
      421 cggcgtgaag tgcgcggtgt gctcgagccc cggcggcagt ccgcccgcca tcgagaagcc
      481 cggggtgccg atcgccctca ccgcgatctc gcgcaggctc ttcctcacgt ccggcgcgct
      541 tttcagccgc acgtagccgt cggcgagctc gagatcatcc tcgacgcact ccatcattcc
      601 cgccgcgatt tttttcgcct tggcggcgac ttccattgcg gcgacatgca ccgaggagcc
      661 ggcattgacc gcggtgcgcg ccgcgaaggt gccgatgccg agcgagatgg cggcggtgtc
      721 ggcggtcacc acggccacat cgttgtaatc aacccccaat tggtcggccg caatctgcgc
      781 cagcgtcgtc ttgtgcgact gtccctgcgg cgtggcgccg gtgtagacga cgatcttgcc
      841 ggaggtcgcg acacggaccg tcgcgccctc gtacggcccg aggccggtcg cctccaccgc
      901 attcgagatg ccgatgccga gataacggcc cgccttgcgc gccgcatccc gccgcgcaac
      961 gaatccacca tggtcggccg cctcgagcgc ggccgcctgg cacgccggat agtcgccgct
     1021 gtcgtaggtg accggccgcc cgtcgcggaa gatgatgccg acgttgtacg gcatctgctc
     1081 cggccggatg aagttgcgcc ggcgcacttc ggccgggtcc agccgcaact cgcgcgccac
     1141 gcggtccatc agccgctcca tggtcaccac cgcctcgggc cggcccgcgc cgcgcaccgg
     1201 tgtggtctgg atcttgttgg tgaacaccga gacgacgtcc agcttgaaat tcgggatcac
     1261 atacggcccc ggcaccgtgg tcgcggtgat ccacggcagc acgatgcccc acggcacgta
     1321 cgcgccggtc tcgtggacca gctggccgcg aacgccgatg attttcgcgc cggcatcgac
     1381 ggctatctcc acgtcccagt actggtcgcg ctcctggtgc gtggcaagga agttctcgcg
     1441 ccggtcctcg atccacttca cgggccggtc ggtgagccgc gccgccacgg cgacgcagac
     1501 gtattcagga tagtacgacc ccttgggccc gaagccgccg cccacgtcag gcgcgaccac
     1561 acgcacctgg tggtcgccga gatcgaacat gtcgagcatg cagcgcttga gccggtgcga
     1621 gccctgcgag gaaacataca gcaggtacga ttgcgccggc gcgtcatagc gcgcgaccgc
     1681 cccgcggcac tccatgaaaa acggcccgcc gcggtgctgg tggattctct cgcggaacac
     1741 gtgggcggcg gcgccgaacg cgcggtcggc atcgccggcc ttgacgggaa aacgcgccgc
     1801 gacattggac tcggcgcccg catgcgcgag cggtgtcccc ggctcgagcg cggtgcggca
     1861 gtcgttcacc gccggcaacg gctcgtagtc gacctcgacc cgttgcgcgg cgtcctcggc
     1921 gatgtagcgg ctctccgcga tcacgcacgc gaccggttcg ccggcatagc aaacctcgtc
     1981 cttcgcgagc gcgtacggca tgtacggttg cttgatcgcg ggatgcggca ccagcagcgg
     2041 cagcgtctgc cgacgcacgg actccggcag atcggcatat gtgaggaccg cgtgcacgcc
     2101 gggcacggcg agcgccgcga ccttgtcgat cctgccgact ttcgcgtgcg cgtaagggct
     2161 gcgcacgaac gcggcgtgca gcataccggg caggtggatg tcgtccacga actgtccttc
     2221 gccgcgcagc agggcggggt cctccttgcg cttgacggac gcgccgaagc cggtgaaggt
     2281 cacgagccgg tctcccgcat ccatttcgcc gccgcgagcg ccgcatcgac gatcccctgg
     2341 tagccggtgc agcggcaaat attgccggac agcgcctcgc gcacttcggc ttcacccggc
     2401 tcgggatggt cgcgcagaaa ctcagtcaat gtcatcaaaa tgcccggcgt gcagaaaccg
     2461 cactgcaggc cgtgatggtc ccggaatgcc tgctgaagcg gattgagttt gtcgccgtcg
     2521 gcgagcgact cgacggtcgc aacctcctcc ccgtcgcact gcaccgcgag catcaggcat
     2581 gcgcgcacgc tcgcgccgcc caccagcacc gtgcacgcgc cgcacacgcc gtgctcgcaa
     2641 cctacgtgcg tgccggtgag attcagctca tgacgcagaa aatcgaccag cgtcaggcgc
     2701 gtatcgattt cgcgcgtgta ctcctcaccg ttgaccgtga tggcgatggt tctggtatca
     2761 ggcattcgcc atctccagcg cgcgcgccga ggcctgctcc agcgcgcggt aggtgagcac
     2821 gcgcgcgagg tgctggcggt acgcggcggt gacgtacgcg tccgcagtgg catcgagctt
     2881 gcccgccgcc gccgccgcgg ccttgaacgt ggccgcgccc ggcttctctc cccgcagcgc
     2941 ctgctcgaca gccgcgggcc gcaccggcgc atgggacagg ccggacagcg cgatcgccgc
     3001 gtccgcgatc cggccgctgc ggtcgagccc gatcagcgcg gagctcgcga cgatggcgaa
     3061 gtcgccgtga cgccgcgcga actcgacgaa cgcgtggccg cggccgcgcg cgggcagcgg
     3121 cagcgtgatg ccggcgagca gctcgtccgg ccgcaggctc gtcgtcatgt agccgaccgc
     3181 gaattcggcg aacgggatgt cgcgcgtgcc gcgcttcgac cttgcatgca gtcggccgcc
     3241 gagcagcgcc gccacgttga cgagctccgc cgacggatcg aggtggcaca gcgagccgcc
     3301 gaaggtgccg cggttgcgcg tctggcggtg cccgacgtgg tcgagcgcct cgcgcaggac
     3361 cgggcagcgc ttgccgactt cttccgagaa ttcgagctcg cgctggcggg tcatcgcgcc
     3421 gacatgcagc gcgtcgccct cacagcgcag gtacgcgagc tcgccgacct tgttcaggtc
     3481 gatcaggtga tccggcatca cgtagcggaa attcatcatc ggcactagcg actggccgcc
     3541 cgcgagcacg cgcgcgttct cgtgcgtcgc gagcaaatcg caggcttccg ccgcggtgcg
     3601 cggatcgtgg tagataaatg gcgccggttt catgaagtga tttggaaccg cagatacacg
     3661 cagacaggtc aaatcaggac gacccctcga ttgaggatta aatccgcgtt cacctgtatt
     3721 gatccgcagt caatcgctct cttgttccgg gtcgaggtat ttgaccttgc gcactggcgt
     3781 ttccggcgtg acgtcgctgc gccgccgccc tatggtctcc ttgccgttct cctcccagtc
     3841 gccggcggtc gcgcgccgcg cgtagcgctc ccaggcatcg gtccagtcgc cgagcgt
//
LOCUS       DBNGWZ010000094         5230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2149268, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000094 DBNGWZ010000000
VERSION     DBNGWZ010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2149268"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16CX_02840"
     CDS             complement(<1..261)
                     /locus_tag="AC16CX_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR3011478.1"
                     /translation="MRNTELYGGLVRLHILYHASKEPVFGLGIMEELRRHGYKLSAGT
                     LYPMLHSMEDAGYLRSRPRIVDGKIRRNYLITARGRNALAAAK"
     gene            complement(287..1516)
                     /gene="chrA"
                     /locus_tag="AC16CX_02845"
     CDS             complement(287..1516)
                     /gene="chrA"
                     /locus_tag="AC16CX_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3011479.1"
                     /translation="MPHPGTLPQEATKNRGSAGAVFAAFLKLGFTSFGGPIAHLGYFR
                     EEFVVRRKWIDDRAYADLVALCQFTPGPASSKVGIGIGLAQAGLPGAFAAWLAFTMPS
                     ALALLLFAYGVESFAGDLGAGWLHGLKVVAVAVVAQAVWGMAKNLCPDAPRFTLAVVA
                     AAITMVWPTSVGQVTAIAAGAVAGCAFLRAKRDEAPAGMQISLGKGAGALSLALFFFL
                     LLGLPAAAAVYPSQPLALFDSFYRAGSLVFGGGHVVLPLLQSEVVPPGWVSNDTFLAG
                     YGAAQAVPGPLFTFAAYLGAVMGPEPNGWQGGLLCLVAIFLSSFLLVIGVLPFWDDLR
                     RYQAVRSALTGVNAAVVGLLLAALYSPVWTSAIKGTADFGLGLAAFALLVFWKLPPWM
                     VVVLSAVCGWGLEKFAL"
     gene            complement(1783..2148)
                     /locus_tag="AC16CX_02850"
     CDS             complement(1783..2148)
                     /locus_tag="AC16CX_02850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3011480.1"
                     /translation="MNAQELKAIDPALGLMGEFSKVTRNTAGHIQKMRQEAVFTDGVF
                     PAKVKALAAALWGISARCEPCLKFYVLKAKELGATEQEIGEFLAVASAMGGCVGEMWA
                     LKAYQAARGASEPQDACCV"
     gene            complement(2178..2354)
                     /locus_tag="AC16CX_02855"
     CDS             complement(2178..2354)
                     /locus_tag="AC16CX_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR3011481.1"
                     /translation="MTSVKRSLTKVNGVANADVSLEKAEAVVTFDDTKTSTDALLKAT
                     KDAGYPSTLKGGRK"
     gene            complement(2487..2891)
                     /locus_tag="AC16CX_02860"
     CDS             complement(2487..2891)
                     /locus_tag="AC16CX_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003116827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HIR3011482.1"
                     /translation="MLQSKVESAHLPERADTDMANGTKSGALAVAGTAGLLASACCIG
                     PLVLASIGLGAAAGGIVAVFEPLRPGFIVIALAALAFAGWGLYRRPVEACETDIACAQ
                     PRTDRIYKMVFWVVAAVVLALITFPYYTELFY"
     gene            complement(2925..>5230)
                     /locus_tag="AC16CX_02865"
     CDS             complement(2925..>5230)
                     /locus_tag="AC16CX_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3011483.1"
                     /translation="GVDRVAVSLTHEQALVEFDPSVVQAEELLRTLRDIGYTVHDPRK
                     LRPFEEEERELVREGRRFLAATAMSLVAIAFIVNPAVPWSVAVDVIATASLAAFAALV
                     LWSRGAVLALAAALALGALAVALLVLRTQGAIAPVAVPWIIGALAFGIVFGTARHIFM
                     MAFQSVLRGILNQHVLLEAAALAGLAGGVFGLAFGRDGYPIAPFFAVAVLVSTYHLFS
                     EWLSLIVKTRSSQAVKRLLDLRPDTARVVRGGREVEVPVEAVAVGDLVRVRPGERVPV
                     DGVVTEGRSAVDLALVTGEPIPVERGGGDAVVGGSINGTGTLLVRVTAVGEESFLQRI
                     AREVEDARALKPGILHLVDRVLRVYAPLVLSVALLAGLAWSLGAYLILGHADLERAIF
                     AGLSALVMGYPCAVGISAPLSIVRGAGEAAERGILMRTGEAFQGYRLVTKIVLDKTGT
                     LTEGRPSLRKTVTADADENELLAIAAAVESASEHPLARAIVQAAFERGVTPPAVDGFE
                     AVPGLGVVARIEGAETLVGSPRFLLERGVSLDALHARIVALEKDAHTVIAVARAGRCV
                     GLLALGDSLRPDARQAVAALRAAGLEPILVTGDNERTARAIAAQLGIETIHAGVLPGE
                     KAEIVRRLQAKGRVAMVGDGINDAPALMQADVGIAMGSGTDIAIDSADIIVLASRLTA
                     LAEAREISRASYRKMLQNVTLAFLFNGIGVPLAATGLVYPVWAMAAMAASVTLIFFNS
                     LWGRPAMFFDAIMSVGRPIAEARPATA"
BASE COUNT          999 a         1758 c         1632 g          841 t
ORIGIN      
        1 cttcgcagcg gccaacgcgt tacgccccct ggccgtgatc aggtagttgc ggcggatttt
       61 cccgtccacg attcgcggcc gggagcgcag gtatcctgca tcctccattg aatgcagcat
      121 cgggtaaagc gttcccgcgc tcaacttgta gccgtgacgg cgcagctctt ccatgatccc
      181 gagtccgaaa acaggctcct tgctcgcatg atagagaata tgcaagcgaa ccaggccccc
      241 gtacagctcg gtgtttctca tgactttccg gccacgcctg ctcttctcat agcgcgaact
      301 tttccagacc ccatccgcag acggcactca gcaccacgac catccagggc ggaagcttcc
      361 agaataccag caacgcaaag gccgccaggc ccaatccgaa atcggctgtt cccttgatcg
      421 cgctcgtcca caccgggctg taaagagcgg cgagcagcag gccgacgacg gccgcgttca
      481 cgccggtcag cgccgagcgc acggcctggt atcgtctgag atcatcccag aacggcagca
      541 cgccgatgac gagcaggaac gaggacagga agatcgccac gaggcaaagc aaacctcctt
      601 gccagccgtt cggctccggc cccatcactg cgcccagata ggcggcgaac gtgaacagcg
      661 gcccgggaac cgcctgggcg gcgccataac cggcgagaaa ggtgtcattg gaaacccagc
      721 cgggcggaac gacctcggac tggagcaggg gcagcaccac gtgtccgccc ccgaacacca
      781 gggagccggc ccggtagaaa ctgtcgaaca gcgcgagggg ctggctggga tagacggctg
      841 ccgccgccgg gaggccgagc aggagaaaga agaacagcgc aaggctcaac gcaccggccc
      901 cttttccgag agatatctgc atcccagccg gcgcctcgtc gcgctttgcc cggagaaacg
      961 cgcaacccgc cacagcgccc gcagcgatcg cggtcacttg tcccacggaa gtcggccaga
     1021 ccatggtaat ggccgcggcc acgaccgcaa gcgtgaagcg gggagcatcc gggcaaaggt
     1081 tcttcgccat gccccacacc gcctgggcga cgacggccac cgcgacgacc ttgaggccgt
     1141 gcagccagcc ggctccgaga tcacctgcga agctttcgac accatatgcg aacagcagga
     1201 gcgcgagggc ggagggcatg gtgaaggcca gccaggccgc aaatgctccg ggcagccccg
     1261 cttgcgcgag cccgatgccg atcccgacct tgctgcttgc ggggcctggc gtgaactggc
     1321 agagcgccac caggtcggcg taggcgcgat cgtcgatcca tttcctacgg acgacgaact
     1381 cttcgcggaa atatcccagg tgggcgatgg ggcccccgaa ggacgtgaat cccagcttta
     1441 ggaaggccgc gaagacggca ccagccgacc cgcgattctt tgttgcttct tgagggagcg
     1501 ttccgggatg cggcaccttg acctcctgcg gcgagttgca cggtttgata tctgtattcg
     1561 atatcgataa tcgatataat agcaccagag cgttaggggt gaagggtgga atcggccgct
     1621 ggccgggcct tgcagtggga ttagagccgg tgtgttaatt tctgcgctgt gcagtcggta
     1681 tgcgcgcaag tgtgctccgc gaactgcgcg gcagcggagt gaagaccacc tgagggggcc
     1741 aagaaacggg atcagagcgg cgtgctgatg cccgccacgc gttcagacgc agcacgcatc
     1801 ttgcggctca ctggcgcctc tggctgcctg ataagccttc agtgcccaca tctcgccaac
     1861 gcagccgccc atcgcggatg cgaccgcgag gaattccccg atttcctgtt cggttgctcc
     1921 cagttccttg gctttgagga catagaactt caggcaaggc tcgcatctag cgctgattcc
     1981 ccaaagcgcc gcggccagcg ctttcacctt tgcggggaac accccgtccg tgaacaccgc
     2041 ctcctggcgc atcttctgga tatgcccggc ggtgttcctg gtcacctttg agaactcgcc
     2101 cattaatcca agggcggggt cgatcgcctt gagttcttgc gcgttcatgg tttcactctc
     2161 ttgattgaaa tgagaattta tttccgtcca cccttaagag ttgacgggta gccggcatcc
     2221 ttggtcgcct tgagcagcgc gtcggtactg gttttcgtgt cgtcgaaagt gaccacggcc
     2281 tccgctttct caaggctcac gtccgcattg gccacgccgt tgactttggt taacgacctc
     2341 ttcacggagg tcacgcacgc gcttcacgtc atccctgaca cggaaagcgt gacgatcttg
     2401 atctccgcga aggcagcagt cgtgaacagg gcggccgccg tgaacgacaa ggcgaggcgt
     2461 ttggcgagtc gtttcatgta tggctcctaa tagaacagct ctgtgtagta gggaaatgta
     2521 atcaacgcga gcacgacggc cgcaacgacc cagaagacca tcttgtagat acggtctgtt
     2581 cgaggctggg cgcaggcgat gtcggtttcg caggcctcaa ccgggcggcg gtatagcccc
     2641 catccggcaa acgcaagcgc cgcgagcgcg atgacgataa aacctggacg cagcggctcg
     2701 aatacggcca cgattccgcc ggcggcggcg ccgaggccaa tcgaggccag cacgagtggc
     2761 ccgatacagc atgccgacgc gagcaatccg gcggtgccgg cgacggcgag cgcgcctgat
     2821 tttgttccat tcgccatgtc ggtatcagcc ctctcaggca aatgcgctga ttcgaccttc
     2881 gattgcagca tgtaatctct cctgtgaaaa aaccgcgcct ggcttcacgc cgtggccggc
     2941 cttgcctcgg cgatcggcct gccgacgctc atgatcgcgt cgaagaacat cgcgggccgc
     3001 ccccacagcg aattgaagaa gatcagcgtc acgctcgccg ccatcgctgc catcgcccat
     3061 acgggataga ccagtccagt ggccgcgagc ggcacgccga ttccgttgaa aaggaacgcg
     3121 agcgtcacgt tctgcagcat cttgcgatag ctcgcgcggc tgatttcgcg cgcttccgcg
     3181 agagcggtca ggcgagaggc gagcacgatg atgtcggccg aatcgatggc gatgtcggtg
     3241 ccgctgccca tcgcgatgcc gacgtcggcc tgcatcagcg ccggcgcatc gttgatgccg
     3301 tcgccgacca tcgcgacgcg ccctttggcc tgcaggcgcc gcacgatctc ggccttctcg
     3361 ccgggcagca caccggcgtg tatggtctcg ataccgagct gcgccgcaat cgcgcgcgca
     3421 gtccgctcgt tgtcgcccgt gacgagaatc ggctcgagtc ctgccgcgcg tagcgctgcc
     3481 actgcctgcc gagcgtccgg gcgcaggctg tcgccaagag caagcaggcc aacgcagcgg
     3541 ccggcacgtg cgaccgcgat gaccgtgtgt gcgtcctttt ccaatgcaac aatgcgtgca
     3601 tgaagtgcgt cgaggctgac gccgcgctca aggagaaatc gggggctgcc gaccagggtt
     3661 tcggctcctt cgatgcgggc gacgacaccc aggcccggaa ccgcctcgaa gccgtccact
     3721 gcgggaggcg tgacaccgcg ctcaaatgcg gcttgcacga tcgcgcgcgc gagcggatgc
     3781 tcggaagcgg actccaccgc agccgcgatc gcgagcagtt cgttctcgtc agcgtccgcc
     3841 gtcacggtct tccgcagcga tggacggcct tcggtgagcg ttcccgtctt gtcgagcacg
     3901 atcttggtta ccaggcgata gccctggaac gcttcgccgg tgcgcatcag gatcccgcgc
     3961 tcggcggctt cacccgcgcc gcgtacgatg gacagcggcg cggagattcc gaccgcgcac
     4021 gggtacccca tcacgagcgc gctgagcccc gcgaatatag cgcgctcaag gtcggcgtgg
     4081 ccaaggatga gatacgcacc gagcgaccat gccagccccg caagaagggc gaccgagagc
     4141 acgagtggcg catagacgcg cagcacccgg tccaccagat gcagtattcc gggcttcagt
     4201 gctcgcgcat cctcgacttc acgcgcgatg cgctgcagga acgactcctc gcccaccgcg
     4261 gtcacgcgga ccagcagtgt gcccgtgccg ttgatggagc ctccgaccac cgcgtcaccc
     4321 ccgccgcgct ctaccgggat cggctcgccg gtgacgagtg caaggtccac ggcggaacgc
     4381 ccttcagtga ccacgccatc caccgggacc cgctcgccgg gccgtacccg cactaggtca
     4441 cccaccgcca cggcctccac cggcacctcc acctcgcgcc cgccgcgcac cactcgcgcg
     4501 gtatccggcc gcaggtcgag caggcgcttc accgcctgcg agctgcgcgt cttaacgatg
     4561 agcgacagcc actcggagaa gaggtgatag gtggagacga gcaccgccac cgcgaaaaac
     4621 ggcgcgatcg ggtaaccatc ccgaccaaac gcgagcccga acacgccgcc ggcgagcccc
     4681 gcgagcgccg cagcctcgag cagcacgtgc tggttgagta tgccgcgcag caccgactgg
     4741 aacgccatca tgaagatatg ccgcgccgta ccgaacacga tcccgaacgc gagcgctcca
     4801 atgatccagg gaacggcgac cggcgcgatc gcgccctgcg tgcgcaggac gagcagagcg
     4861 acagcgagcg cgccgagagc gagtgcagcg gcgagcgcca gtaccgcgcc gcgcgaccag
     4921 agtacgagcg cggcgaatgc ggcaaggctc gccgtggcga tgacgtcaac tgccaccgac
     4981 caagggaccg ccgggttgac gatgaaggcg atcgccacga ggctcattgc cgtcgcggcg
     5041 agaaaacgcc ggccttcgcg caccagttcg cgctcctcct cctcgaacgg ccggagcttg
     5101 cgcgggtcgt gtaccgtgta accgatgtcg cgcagcgtcc gcagcagctc ctcggcctgc
     5161 accacgctcg gatcgaattc caccagcgcc tgctcatggg tcaggctcac cgcgaccctg
     5221 tccacgccgg
//
LOCUS       DBNGWZ010000095         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2401246, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000095 DBNGWZ010000000
VERSION     DBNGWZ010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2401246"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..>1567
                     /gene="purB"
                     /locus_tag="AC16CX_02870"
     CDS             210..>1567
                     /gene="purB"
                     /locus_tag="AC16CX_02870"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3011484.1"
                     /translation="MASGVFDDALIKHLWSTEELRAIFSDRNRVQKWYDFEAALALEQ
                     AELGIIPPAAAREIAAKARVENVDIEAIAAEIRRIKHPLVPALKAVEKLCQDGHGEFI
                     HFGPTTQDVLDTGTVLQIKDAHAVYLRDVKAIGRALYELAETHKTTPMAGRSHGVQAL
                     PITFGHKAAIWLSEMGRSYRRLRDLEPRVFVGGMVGAVGTQASYGPQAPELERRVMKR
                     LALGIADINWQPARDRFAEYVCVLAIIGQTLGKIANEIINLEHTEIGEVFEPFSEGKV
                     GSSTMPHKRNPSTCEAAVGVSRALRYNAAFMLECMVIEHERDGSAWRGEWKALPESCL
                     MAGGLLAAMKYVLAGLHVDAERMRKNLDLLGGFLLSERVMFALAEKVGKQTAHELVYE
                     ASMYGIERGISFEKALLQNRKVKEALSADALRAALDPTTYVGLAPQIVDRVLAETRAS
                     GWL"
BASE COUNT          298 a          487 c          545 g          237 t
ORIGIN      
        1 tcggcggcac gcccgagcag ttcgcggcct acatccgcga cgagatcgcc aaatgggcca
       61 aggtcatcaa gttctccggt gcacgggcgg actgagggga tgcggcagat gaacgtgaga
      121 gcgggagagc gggagagcgg gagagcgaaa accttcgggc attgatccca ctcacgatct
      181 caccacctca cgaactcacg gattcaagta tggcctccgg cgtattcgac gacgcgctga
      241 tcaagcacct gtggagcacg gaggagctgc gcgcgatctt cagcgaccgc aatcgcgtgc
      301 agaagtggta cgacttcgag gcggcgctcg cgctggagca ggctgagctc ggcattattc
      361 cgcccgccgc cgcgcgggaa atcgccgcca aggccagggt cgagaacgtg gatatcgaag
      421 cgatcgccgc cgagatccgg cgcatcaagc acccgctggt tccggcgttg aaggcggtcg
      481 aaaaactgtg ccaggacggg cacggcgagt tcatccattt cggcccgacc acgcaggacg
      541 tgctcgacac cggcaccgtg ttgcagatca aggacgcgca cgcggtctac ctgcgcgacg
      601 tgaaggcgat cggccgcgcg ctttatgaac tggccgagac gcacaaaacg acgccgatgg
      661 cgggccgctc ccacggcgtg caggccttgc ccatcacctt cggccacaag gcggcgatct
      721 ggctctccga gatgggccgc agctaccggc gcttgagaga cctcgaaccg cgcgtgttcg
      781 tgggcggcat ggtcggcgcg gtgggcacgc aggcctcgta cgggccgcag gcgccggagc
      841 tcgaacgccg ggtgatgaag cggctcgcgc tcggtatcgc cgacatcaac tggcagccgg
      901 cgcgcgaccg cttcgccgaa tacgtctgcg tgctggcgat catcggccag acgctcggaa
      961 agatcgcgaa cgagatcatc aacctcgagc acaccgagat cggcgaagtg ttcgagccgt
     1021 tcagcgaggg caaggtgggc tcctccacca tgccgcacaa gcgcaacccc tcgacttgcg
     1081 aggcagcggt gggcgtgagc cgggcgttgc gctacaacgc ggcgttcatg ctcgagtgca
     1141 tggtgatcga gcacgagcgc gacggctcgg cgtggcgcgg cgagtggaag gcgctgcccg
     1201 aatcctgcct gatggcgggc ggcctgctgg cggcgatgaa gtacgtgctc gccgggctgc
     1261 acgtcgatgc ggagcgcatg cgcaagaacc tcgatctcct cggcggtttc ctgctctccg
     1321 agcgcgtcat gttcgcgctc gcggagaagg tcggcaagca gaccgcccac gaactggtgt
     1381 acgaagcgtc catgtacggc atcgagcgcg gcatcagttt tgagaaggca ctgctgcaaa
     1441 acaggaaagt gaaggaggca ttgtccgctg atgcgctacg ggccgcgctc gatccgacga
     1501 cttacgtcgg cctcgcgccg cagatcgtgg acagggtgct ggccgagacg cgggcgagcg
     1561 gatggct
//
LOCUS       DBNGWZ010000096         3579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3282911, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000096 DBNGWZ010000000
VERSION     DBNGWZ010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3282911"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="AC16CX_02875"
     CDS             complement(<1..1070)
                     /locus_tag="AC16CX_02875"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3011485.1"
                     /translation="MRIFVLGAGATGSLLARLLVRQGHRVTCGDRDPDRARRFLGRKS
                     RIPIRQVNARNLWSIVKAARGSHLIVNASPAVLNKMVLRAALRLRAHYLDTASHLADS
                     PFRAEQLRFDARFRAKRRAAVINAGVAPGLTNLLVARSADLLNGVEAVHIRLYESTGS
                     DDPVSQWSPDAAFDEAVSRPGVYRHGRFRLGKRFGEREAFRFPPPVGRVGVVLAAQDE
                     VATLPRVLGMREMDVKIGGSDFERMRRWYRQGMLRRSHGPAANRFPQTPTPRQVARLV
                     SRGVLYHARFAAAVMVRGRKGERRAVIRWDAGFPTLHQIRRREVHTSPIAWATAHLAA
                     LFVKHFPRNSPGVHPPEALPART"
     gene            complement(1095..1415)
                     /locus_tag="AC16CX_02880"
     CDS             complement(1095..1415)
                     /locus_tag="AC16CX_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HIR3011486.1"
                     /translation="MICGSFRSERHSNMKRASTSRKSRSDWARVRSLKDKDIVVDRDA
                     PEWTPEMFARAVVKKGLKTPSSKSLLSLRIDSEVLSWFRSQGPGYQSRMNALLRAYME
                     AHKT"
     gene            complement(1622..3010)
                     /locus_tag="AC16CX_02885"
     CDS             complement(1622..3010)
                     /locus_tag="AC16CX_02885"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011487.1"
                     /translation="MTLALELAKRINAPTFDELPREAIHWAKVGILDTVGVTLAGSRE
                     DTTRIVENVTLYDMAGPSLIFGSGRRASPLDAALVNGTAAHALDFDDMNNTLGGHPSV
                     PILPALFALAEDIGANGRDFIAAYVAGFETECKIGLGVHFHHYTKGWHPTATLGVFGC
                     AAACARLMKLSDEKLATALAIAASLAAGVKANFGTMTKPLHVGHCTRSGLFAALLARD
                     GFTASDRAFEHKQGFFNVFNGEGNYDAGKILPDWAKPLDIVKPGIAIKQYPCCGSTHP
                     ALDATLTLAREHKPAADEIERVDVWIHERRLEHTNRPEPRSALDAKFSLQYCVARALI
                     DRRIAIEHFEGRAYDEPAVRKLLARVHAAPYTTAQFPAENHFGAEVKIALRSGKALEA
                     KVDQPFGRTAENALPQEQLKEKFENCAARVLPRERIAPLYAAIQDFENLKDVRELMAI
                     IAGEGRRARAAA"
     gene            complement(3088..>3579)
                     /locus_tag="AC16CX_02890"
     CDS             complement(3088..>3579)
                     /locus_tag="AC16CX_02890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3011488.1"
                     /translation="GGIAHLGGAWLGSIAGIEMVHIPYKGGAPATLDLIAGRTQMQLG
                     TFLLTGPHIRAGKLRVLAVTTPQRWPGTPDLPTMQEAGVPGFAITQWYGMLAPAKTPP
                     AIVSRLSKEISRLLHQPDVKEKLATDGADAVGSSPAEFGAHIRTEVVKYAKIVKAVGL
                     KAE"
BASE COUNT          561 a         1126 c         1214 g          678 t
ORIGIN      
        1 gtcctggctg gtaaagcttc cggtggatgg acgccgggcg agttgcgtgg aaaatgcttc
       61 acgaacagcg cggcgaggtg ggccgtggcc cacgcgatcg gtgaggtatg gacctcgcgg
      121 cggcggatct ggtgaagggt ggggaagccg gcgtcccaac ggataaccgc acgccgttcg
      181 cctttccggc ctcgcaccat cacggccgcg gcaaagcgcg cgtggtacag cacgccgcgg
      241 gagaccagcc gcgccacctg gcgcggtgtc ggggtctgcg gaaaacgatt tgccgccggg
      301 ccgtgcgacc tccggagcat gccctgccgg taccagcggc gcatgcgctc gaaatcactc
      361 cccccgatct tgacgtccat ctcgcgcatg ccgaggacgc gcggcagcgt ggcgacctcg
      421 tcctgcgcgg cgagcacgac gccgacccgg ccgacgggag gcggaaagcg gaacgcctcg
      481 cgctcgccaa agcgcttgcc taggcggaaa cgaccatggc ggtagacgcc gggccgcgac
      541 accgcctcgt cgaaggccgc gtcgggagac cactgcgaga cgggatcgtc gctgcccgtg
      601 ctttcgtaga gccgtatgtg gacggcctcg accccgttga gcaggtccgc gctgcgcgcg
      661 acgagcaggt tcgtgaggcc cggagccacg ccggcattga tcactgcggc gcggcgtttc
      721 gcccggaagc gtgcgtcgaa gcggagctgt tcggccctga agggggagtc ggccaggtgc
      781 gaggcggtat cgagatagtg ggcgcgcagg cgcaacgcgg cgcgcaggac catcttgttc
      841 aagacggcgg ggctcgcatt gacgatcagg tgactgcccc tcgcagcttt tacgatgctc
      901 cataggttgc gcgcgttcac ctgccggatc gggattcgcg acttcctgcc gaggaagcgg
      961 cgtgcgcgat ccgggtcccg gtcgccgcac gtgacccggt gaccctggcg gacgagcagc
     1021 cgcgcgagca gcgagccggt tgcgccggct ccgagaacga agatccgcat cggatttata
     1081 ggatcagcga gatctcacgt cttgtgtgct tccatgtacg cgcgaagaag cgcgttcata
     1141 cgtgattgat aacccggccc ctgcgatctg aaccaggaaa gaacctcgga atcgattcga
     1201 agcgacagaa gtgacttgga cgaaggtgtc ttgagtcctt tcttaacgac ggcccgcgcg
     1261 aacatttccg gagtccactc gggcgcgtcg cgatcgacga caatgtcctt gtccttcaga
     1321 gaccgcactc gggcccagtc acttcgagat ttgcgagagg tagaagcgcg tttcatgttt
     1381 gagtgccttt cggatcgaaa tgatccgcat atcatccttc cgttcagtgc gtattttcag
     1441 cggttcgcgc gttgagcgga gaggagaaag ttacaccgag gtccctctga agtccctgca
     1501 actggatcag agttaaagac tacgtttcaa tccaatcttg ttcttggttt tctcggcggt
     1561 tcaatgtttg tcgttgctat gtgcccttcg tgtccttggt agttcagctt tctcgatttg
     1621 gtcaagccgc cgcccgcgcg cgcctgcctt cacccgcgat gatcgccatc agctcgcgca
     1681 cgtccttcaa gttctcgaag tcctggatcg cggcgtagag cggcgcgatg cgctcgcgcg
     1741 gcagcacccg cgcggcgcag ttctcgaatt tctccttcag ctgctcttgc ggcagcgcgt
     1801 tctcggcggt gcggccgaac ggctggtcca cctttgcctc caacgccttg ccgcttcgca
     1861 gcgcgatctt cacttctgcg ccgaagtggt tttccgccgg gaactgcgcg gtggtatagg
     1921 gcgcggcgtg cacgcgcgcg agcagcttgc gcaccgccgg ctcgtcgtac gccctccctt
     1981 cgaaatgctc gatcgctatg cgccggtcga tcagcgcccg cgcgacgcag tactggaggc
     2041 tgaacttggc gtcgagggcg ctccgcggct cgggacgatt cgtgtgctcg agccgccgct
     2101 catggatcca cacgtccacc cgctcgatct cgtcggcggc aggcttgtgt tcgcgcgcga
     2161 gcgtaagtgt cgcatccagc gcggggtgcg tgctgccgca gcagggatat tgcttgatcg
     2221 cgatgccggg cttgacgatg tcgagcggtt tcgcccagtc ggggaggatt ttcccggcgt
     2281 cgtagttgcc ctcgccgttg aacacgttga aaaaaccctg cttgtgctcg aaggcgcggt
     2341 cgctggcggt gaagccgtcg cgcgcaagca gcgcggcgaa caacccgctc ctcgtgcaat
     2401 ggccgacgtg cagcggcttg gtcatggtgc cgaagttggc cttgacgccg gcggcaagcg
     2461 aggcggcgat ggcgagcgcg gtggcgagct tctcgtcact cagcttcatc agcctggcgc
     2521 aagccgcggc gcagccgaag acgccgagcg tggcggtcgg gtgccagccc ttggtgtagt
     2581 gatggaagtg gacgccgagc ccgatcttgc actcggtctc gaagccggcg acgtaggcgg
     2641 cgatgaaatc gcggccgttc gcgccgatgt cctcggcgag cgcaaacagc gccggcagga
     2701 tcggcaccga gggatggccg cccagcgtgt tgttcatgtc gtcgaagtcg agcgcatggg
     2761 cggcggtgcc gttcaccagc gccgcgtcga gcgggctcgc ccgccggccg ctgccgaaga
     2821 tcaggctcgg tcccgccatg tcgtacaacg tgacgttctc cacgatgcgc gtcgtgtcct
     2881 cacgcgatcc ggcgagcgtc acgcccaccg tgtcgaggat gccgactttt gcccagtgaa
     2941 tcgcctcgcg aggcaattca tcgaacgtgg gggcattgat ccgcttggcc agttccagtg
     3001 cgagtgtcat attgtctcca gactgcgtga ggcgtgagga gcgaggtgac agggtttccc
     3061 ggtcgtgacg gtcttctcct cacgatttta ttccgccttg aggccgactg ccttcacgat
     3121 ttttgcgtac ttgacgacct ccgtccggat atgcgccccg aactccgcgg gtgagcttcc
     3181 gacggcgtcg gcgccgtcgg tggccagctt ctccttgacg tcgggctggt ggagaaggcg
     3241 tgagatttcc ttgctgagcc tgctgacgat cgccggcggg gtcttcgccg gggcgagcat
     3301 gccgtaccac tgcgtgatgg cgaagccggg cacgccggct tcctgcatcg tgggcaagtc
     3361 cggcgtgccg ggccagcgct gcggggtggt caccgccagc acgcgcagct tgccggctct
     3421 gatgtgcggg cccgtcagca ggaacgtgcc cagctgcatc tgcgttcgtc cggcgatgag
     3481 atcgagtgtc gccggggcgc cgcctttgta cgggatgtgg accatctcga tgcccgcgat
     3541 ggacccgagc caggcgccgc cgagatgggc gatgccgcc
//
LOCUS       DBNGWZ010000097         1735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2779266, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000097 DBNGWZ010000000
VERSION     DBNGWZ010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2779266"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..>1735
                     /locus_tag="AC16CX_02895"
     CDS             56..>1735
                     /locus_tag="AC16CX_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR3011489.1"
                     /translation="MSFNWRTWLAFAHDVAACAAAWMIAFWLRFNLDIPPGYLELALK
                     TLPWVITAYAVLFWGFGLYRGIWRFASLPDLRRILVTVGAGAVAAPALLFMLQLQAPV
                     PRSVFIMSPILLMLMMGGSRIAYRAWKEGMTGGLMSANREPVVVIGAEEAAVSLIKDL
                     ARSPQWNIVGVFDDDHAKVGRQLHGVNVLGRLEEIPRWRGRLGFSQAIIAMPEATHVA
                     RRRAVEISNRAGIKVLTVPSFEDLVSGRVTVSQVRHVELDDLLGRDPVELDAEGLRSW
                     LSGRVVMVTGAGGSIGAELCRQVARFSPQQLVLFELSEFALYTIEQEFAERYPRVPVV
                     PAAGDVKDEARVEQVIRCYRPSVIFHAAAYKHVPLMEKENAWQAVLNNVVGTHIVARA
                     SLACGVEKFVLVSTDKAVNPTSVMGASKRLAEMVCQALQRPEGTRFVMVRFGNVLGST
                     GSVVPRFREQIARGGPVTVTHSEVTRYFMSIPEAVQLVLQAGLMGGGGEIFVLDMGEP
                     VRIADLARDLIKLSGFSEEEIKIVYTGLRPGEKLYEEPLAGNENLLPTPHPK"
BASE COUNT          285 a          552 c          584 g          314 t
ORIGIN      
        1 ctaatcccca ttccctattc cccgttcccc gtctccgatt cgctgccgac aggacatgag
       61 ctttaactgg cgcacatggc tcgccttcgc ccacgatgtg gccgcctgcg cggctgcatg
      121 gatgatcgcg ttctggttgc gattcaacct tgatatccct cctggatatc tcgagctcgc
      181 gctgaagact cttccatggg tgatcacggc atacgccgtc ttgttctggg gattcgggct
      241 ctaccgcggc atctggcgct tcgcgagcct gcccgatctc aggcgcatcc tggtcacagt
      301 aggcgcggga gcggttgccg cgccggcgct gctcttcatg ctgcagctgc aggcacccgt
      361 gccgcgttcg gtcttcatca tgagccccat cctgctcatg ctgatgatgg gcggcagccg
      421 gatcgcttac cgcgcgtgga aggaaggaat gacgggcggg ctcatgtccg cgaatcgcga
      481 gccggtcgtt gtcatcggcg ccgaggaggc cgcggtcagc ctgatcaagg atctcgcgcg
      541 cagcccgcag tggaacatcg tgggcgtgtt cgacgacgac catgccaagg tcggccggca
      601 actgcacggc gtcaacgtgt tgggccggct cgaggagata ccccgctggc gcggccggct
      661 tggattctcg caagcaatca tcgccatgcc ggaagcgacc cacgtcgcgc gccgccgcgc
      721 agtggaaatc agcaaccgcg ccgggatcaa agtgctgacg gtaccctcgt tcgaggacct
      781 ggtgagcggc cgcgtcacgg tctcgcaggt gcgccacgtg gagctcgacg acctgctggg
      841 acgcgatccg gtcgagctcg acgccgaggg cctgcgcagc tggctctcgg gacgggtggt
      901 catggtcacg ggcgccggcg gctcgatcgg cgcggagttg tgccggcagg tcgcgcgctt
      961 cagcccgcag caactggtcc tgttcgagct gagcgagttt gccctctaca ctatcgagca
     1021 ggagtttgcg gagcgttacc cccgcgtgcc ggtggtgccc gcggcaggcg acgtgaagga
     1081 cgaagcgcgg gttgagcaag tcatcaggtg ttaccgcccg tcggtcatct ttcatgctgc
     1141 ggcctacaag cacgtgccgc tgatggaaaa ggaaaacgcc tggcaggcgg tgctcaacaa
     1201 cgtggtgggg acgcacatcg tcgcccgcgc ctccttggcc tgcggcgtcg agaagttcgt
     1261 gctggtctcc accgacaagg cagtcaaccc cacgagcgtg atgggtgcga gcaagcggct
     1321 cgccgagatg gtgtgccagg cgctgcagcg gccggagggc acgcgcttcg tcatggtgcg
     1381 cttcggcaac gtgctgggct cgaccggcag cgtggtgccc aggttccgcg agcaaatcgc
     1441 ccgcggcggc ccggtcaccg tgacccactc cgaggtcacg cgctatttca tgtcgatacc
     1501 ggaggcggtg cagctcgtgt tgcaggcagg tctcatgggc gggggcgggg aaatcttcgt
     1561 gctcgacatg ggcgaaccgg tcagaatcgc cgatctcgcg cgcgatctca tcaagctctc
     1621 cggattcagc gaggaggaaa tcaagatcgt atataccggg ctgcgtccgg gcgagaagct
     1681 ctacgaggag ccgctcgcag gcaacgagaa cctgctgccc acgccgcatc cgaag
//
LOCUS       DBNGWZ010000098         5078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_134265, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000098 DBNGWZ010000000
VERSION     DBNGWZ010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_134265"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="AC16CX_02900"
     CDS             complement(<1..388)
                     /locus_tag="AC16CX_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3011490.1"
                     /translation="MNRTSKPTHDLAVEKDVEIPMRDGARLKPDIFRPESAGRFPVIM
                     NLGGYRKDKVWVPPSDLVEKANPYMNWETVNPLWWIPRGYSAIRVDTRGSGKSPGLFD
                     PWSRQEALDFNDCIEWAAKQKWCNGRV"
     gene            complement(423..1403)
                     /locus_tag="AC16CX_02905"
     CDS             complement(423..1403)
                     /locus_tag="AC16CX_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011491.1"
                     /translation="MSRTIGLTLILLCAAAVAPSAQAAEKFPQRPVRFIVPYAPGGAT
                     DITARLLQIGISELWGQPVIVDNRAGASGTIAVEMAAKAAPDGYTLQVGNVSTNAINE
                     TTFSSLKVKPSRDLTGVTNLIQLPHLWVVSTAVPASSLKEFFDYARKSGGKLNYGSAG
                     VGSYPHLDAVKLLKHAGVQMTHVPYKAGAAQMIPAIMGNEIQFMFINMASSLQNIRAG
                     RIKPLATTWPTRRPELPNVPTVAEAGYPDFGTNAWNGLFAPAGIPKPLLNRIHADVVK
                     VMESPATKDALAKVFMTGVVNRSPEDFQRFVLEEIRIWGKIVTDNNIKVE"
     gene            complement(1448..2563)
                     /locus_tag="AC16CX_02910"
     CDS             complement(1448..2563)
                     /locus_tag="AC16CX_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3011492.1"
                     /translation="MKKQPKRIGLAIVGAGRVGLIRGELAARHPEVDWIGLAEINPER
                     GEIVREKCGADFVTTDFRELLSRPEVNAVVVSTDEHLHVDPVVFAAERKLPMLIEKPL
                     ATDLGESAATLAAIEKSGADAVIGYTQRFRRRWLVAKEKVGSGALGEVTMVTSRAFMN
                     RLVAIDNYKRSDNRAEVTPMVISGTHALDIVMWLMEARKPVEVYARSVNKALGPTWQG
                     VDGTGYTITFDDGAIYHGVISWALPEIWPGAVYSLEIGAVGTEGVLTIDDTHRDIILA
                     TNLIQAEGYAPDKRRHVDFLTSYPVGDMALGELRGPMREETHSWLNRVSLGVTTQHAT
                     AREGHNRLMLTKAIDLSARLKRSVQLPIGPEDEKRVK"
     gene            complement(2610..3680)
                     /locus_tag="AC16CX_02915"
     CDS             complement(2610..3680)
                     /locus_tag="AC16CX_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3011493.1"
                     /translation="MKQINVGIIGTGWCGGIRAETCATHPVVGSLHLAEVRPERLAEV
                     AKATGAKTATADYRELLKIRDIDAYLISATPETHHYPMARECLLAGKHVFLEKPIAME
                     LHEADELIAIARKNKLKFTIGYSQRFNPKFAYVKKSIIDGTIGRPVSALVSRHITRGL
                     GKKITGRTKLSPAAMEATHDLDFVLWCLEPAKPVRVFSTVNYGVMRESTGADIPDTQL
                     ITVIMDNGISFVVNAGWSLPPGYPNFATTWIEMVGTEGALLVDDSHRDVVLNTMREGM
                     RLPMSSMPGEFVDHAYAGPMKAETVHFLEAVACDRPVLVTPEQARQVMEVYIAADLSA
                     ERGEPVNLPLPESRPTAATAAR"
     gene            3997..4842
                     /locus_tag="AC16CX_02920"
     CDS             3997..4842
                     /locus_tag="AC16CX_02920"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3011494.1"
                     /translation="MPFYRFENIESHYLTPHLSTGKAPVIEGQYMYFCLLHKNAGTGS
                     ELHYHPNELLIFPLRGKIKAIVGKDRRIVSPGTFVHAPAYARHSMKATEDGHCQYLYI
                     KDKTWTVVGLAENEAVPERAMTVEEVNRRYKVGERDKHKGAQGKSRAIVEGLDVCFYP
                     VIESLDAPQRSARHVNWIEGERLAFGFFEVPGGYDEPETTAQHEMFIYILSGRLRARS
                     GDQTQLVESGDIVHVSRDSACRLQVESPFARYVLVCSTPYLENRIDNMMPDEAEQARL
                     HIKPN"
     gene            4884..>5078
                     /locus_tag="AC16CX_02925"
     CDS             4884..>5078
                     /locus_tag="AC16CX_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011495.1"
                     /translation="MEAIRLSFRTRLFPVVLGLLMPVAALAQGWKPERNVELIVPASA
                     GGSLDLTGRTVHKIWDDLKLL"
BASE COUNT          861 a         1563 c         1689 g          965 t
ORIGIN      
        1 cgacgcggcc gttgcaccac ttctgtttcg ccgcccactc gatgcagtcg ttaaagtcga
       61 gcgcttcctg gcgcgaccac ggatcgaaca aaccgggaga cttgcctgag ccccgcgtgt
      121 ccacgcggat tgcgctgtag ccgcgcggga tccaccacag ggggttgacc gtctcccagt
      181 tcatgtaggg attggccttc tctaccaggt ccgacggcgg cacccacacc ttgtccttgc
      241 ggtagccgcc gaggttcatg atcaccggga agcggccggc ggactccggc cggaagatgt
      301 cgggcttgag ccgggcgccg tcgcgcatcg ggatctcgac atccttctcg acggcgagat
      361 cgtgcgtggg cttggaggtt ctgttcacgg ccttcggtca tgaaggatgc ggatcgcttc
      421 cttcattcca ccttgatgtt gttgtcggtg acgatcttgc cccatatcct gatttcctcc
      481 aggacgaagc gctggaagtc ctcaggcgag cggttgacca cccccgtcat gaacaccttg
      541 gcgagcgcgt ccttggtcgc cgggctctcc atgaccttca cgacgtcggc gtggatgcgg
      601 ttgagcagcg gcttgggaat gccggcgggc gcgaacaggc cgttccaggc attggtgccg
      661 aagtcgggat acccggcttc cgcgaccgtg ggaacgttcg gcagctcggg acgccgcgtg
      721 ggccacgtgg tggcgagcgg tttgatccgc ccggcgcgga tgttctgcag cgacgaggcc
      781 atgttgatga acatgaactg gatctcgttg cccatgatcg ccgggatcat ctgggccgcg
      841 ccggccttgt acgggacgtg cgtcatctgc acgccggcgt gcttgagcag cttgacggcg
      901 tcgaggtgcg ggtacgaccc gacccccgcc gagccgtagt tcagcttccc gcccgatttc
      961 cgggcgtagt cgaagaactc cttgaggctg gaggccggca cggcggtgct caccacccac
     1021 aggtgcggca gctggatcag attggtgacg ccggtgaggt cgcgcgaggg cttgaccttg
     1081 agcgaagaga acgtcgtttc gttgatggcg ttggtcgaga cgttgccgac ctgcagcgtg
     1141 tagccgtcgg gcgccgcctt cgccgccatt tcgaccgcga tagtgcccga ggcgccggcc
     1201 cggttgtcga cgatgaccgg ctgcccccac agctcgctga tgccgatctg gagcaggcgc
     1261 gcggtgatat cggtggcgcc gccgggggcg tagggaacga tgaagcgcac cggccgctgc
     1321 gggaacttct cggccgcctg cgcggacggc gcaaccgccg ccgcgcataa aagaatcaac
     1381 gtcaaaccga tcgttctgct catggtgtcc tccccggata tccaatggcg gcgtctaccg
     1441 ccatcaatta cttgacgcgc ttttcatcct ccggcccgat cggcagctgg accgaccgct
     1501 tgagccgcgc ggagaggtcg atcgccttgg tcagcatcag gcggttatga ccctcgcggg
     1561 cggtcgcgtg ttgcgtcgtc acgccgagcg agacgcggtt cagccaggaa tgggtctcct
     1621 cgcgcatggg gccgcgcagc tcgccgagcg ccatgtctcc gaccggatag ctggtcagga
     1681 aatcaacgtg gcggcgcttg tcaggcgcat agccttcggc ctggatcagg ttggtcgcca
     1741 gaatgatgtc gcgatgcgta tcgtcaatgg tgagcacgcc ttcggtgccg accgctccga
     1801 tttcgaggct gtagaccgcg ccgggccaga tttcgggcag cgcccaggag atcaccccgt
     1861 ggtagatcgc gccgtcgtcg aaggtgatgg tatagccggt gccatccacg ccctgccacg
     1921 tgggccccag cgccttgttc accgaacgtg catagacctc gaccggcttc ctggcttcca
     1981 tcagccacat cacgatgtcg agcgcatgcg tgccggagat caccatcggc gtcacttccg
     2041 cgcggttgtc gctgcgcttg taattgtcga tggcgaccag ccggttcatg aaggcgcgcg
     2101 aggtcaccat cgtgacctcg ccgagcgcgc cggacccgac tttctccttt gccacgagcc
     2161 agcggcgacg gaaacgctgg gtgtagccaa tcacggcgtc cgcgccggac ttctcgatgg
     2221 cggcgagcgt ggccgcggat tcgcccagat ccgtcgcgag cggcttctcg atcagcatcg
     2281 gcagcttgcg ctcggcggca aaaacaaccg gatcgacgtg caggtgctcg tcggtggaaa
     2341 cgacaaccgc gttgacctcg gggcgcgaga gcagctcgcg gaaatcggtg gtgacgaaat
     2401 ccgcgccgca tttctcgcgc acgatttcgc cgcgctcggg attgatctcg gcgaggccga
     2461 tccagtccac ttccgggtgg cgggcggcga gctcgccgcg gatcaggccg acgcggcccg
     2521 cgccgacgat ggcgaggccg atacgcttgg gttgtttttt catcgctcag gcgtgattcg
     2581 tgaatcgtga ttcgtgggtc gaccggcttt caccgcgcgg cggtcgcggc cgtggggcgt
     2641 gactcgggca ggggcaggtt gaccggctcg ccgcgctcgg cggagaggtc ggccgcgata
     2701 taaacctcca tcacctggcg cgcctgctcg ggtgtgacca gcaccggccg gtcgcaggcg
     2761 accgcctcga ggaagtgcac cgtctccgcc ttcatcggac cggcgtaggc atggtccacg
     2821 aactcgccgg gcatggacga catcggcagg cgcatgccct cccgcatggt attgaggacc
     2881 acgtcccggt ggctgtcgtc caccagcagc gcgccctcgg tgcccaccat ctcgatccag
     2941 gtcgtggcga aattcgggta gccggggggc aggctccagc ccgcgttcac cacgaaggag
     3001 atcccgttgt ccatgatgac ggtgatgagc tgtgtgtcgg ggatgtccgc tccggtggat
     3061 tcccgcatca cgccgtaatt gaccgtcgag aagacccgca ccggtttggc gggctccagg
     3121 caccacagca cgaagtcgag gtcgtgggtg gcctccatcg cggcggggga gagcttggtg
     3181 cgcccggtga tcttcttgcc caggccgcgg gtgatgtggc ggctgaccag cgcggagacc
     3241 ggcctgccga tcgtgccgtc gatgatggat ttcttgacgt aggcgaactt gggattgaag
     3301 cgctgggagt agccgatggt gaatttgagt ttgtttttcc gcgcgatcgc gatcagctcg
     3361 tcggcttcgt gcagttccat cgcgatcggc ttctccagga agacgtgctt gccggcgagc
     3421 aggcattcgc gcgccatcgg gtaatgatgg gtttccgggg tcgccgagat gaggtaggcg
     3481 tcgatatccc ggattttcag cagctcgcga taatccgcgg tagcggtctt tgcccccgtc
     3541 gccttggcaa cttctgccag gcgttcgggc ctgacctccg ccaggtggag gctgccaacg
     3601 acgggatggg tggcgcaggt ctcggcgcgg atgccgccgc accagccggt gccgatgatg
     3661 ccgacgttaa tttgcttcac gtattctccg gacggggttt actacggaca gaaatgcgga
     3721 ttatgtcgca taatgcgccg ctggtgaaag gggattgatg atcggcacgg atttgccgtt
     3781 cgacatgggt gagagcgccc ggtcgacagg ctcaacgccg tgccgcggct gaccgaccgg
     3841 gagcgcgagg acgtttcata ccgcaccgcg ctgcggctgc ttggcgaaat gtgaggcacg
     3901 atacgagtta cgagatgcga gatccgagac gggagatatc cgcttgatcg cgtatcgcgc
     3961 atcttgcatc acgattattt gaggtaagtt gcgaccatgc cgttctaccg ttttgaaaac
     4021 atcgagagcc attacctgac gccgcacctg tctaccggca aggcgccggt gatagagggg
     4081 cagtacatgt atttctgcct gctgcacaag aacgccggga ccggctccga gctgcactat
     4141 caccccaacg aactgctgat cttcccgttg cgcggcaaga tcaaagccat cgtcggcaag
     4201 gaccggcgta tcgtgtcgcc ggggacgttc gttcatgcgc ccgcctacgc gcgccactcc
     4261 atgaaggcga ccgaggacgg ccactgccaa tacctctaca tcaaggacaa gacctggacc
     4321 gtcgtggggc ttgccgagaa cgaggccgtg cccgagaggg cgatgacggt ggaggaggtg
     4381 aaccggcgct acaaagtggg cgagcgcgac aagcacaagg gcgcgcaggg caagtcccgg
     4441 gcgattgtgg aagggctgga tgtctgcttc tatccggtca tcgaatcgct cgacgcgccg
     4501 cagcgctcgg cccgccacgt caactggatc gagggcgagc ggctcgcctt cggcttcttc
     4561 gaggtcccgg gcggctacga cgagccggag acgaccgccc aacacgagat gttcatctac
     4621 attttaagcg gtaggctgcg agcgagatcg ggtgaccaga cccaattggt ggaaagcggc
     4681 gatatagtcc atgtttcccg ggactcggcc tgccgcttgc aggtagaatc gccattcgcg
     4741 cgttatgtgc tggtctgttc gacgccgtac ctggagaacc ggatcgacaa catgatgccg
     4801 gatgaagccg agcaagctcg gcttcatatc aaacccaatt aaagtcgcaa tcgacgggat
     4861 gcgaatcgga taaggcggcg accatggagg cgattcgttt gtcattccgc acgcgcctat
     4921 ttcccgttgt gctcggcctt ttgatgcctg tcgccgcctt ggcgcaaggc tggaagcccg
     4981 agcgcaacgt cgagctgatc gtgccggcga gcgccggcgg ctcgctcgac ctgaccggac
     5041 gcaccgttca caagatctgg gacgacctca aactgctg
//
LOCUS       DBNGWZ010000099         5772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2779330, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000099 DBNGWZ010000000
VERSION     DBNGWZ010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2779330"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..900
                     /locus_tag="AC16CX_02930"
     CDS             208..900
                     /locus_tag="AC16CX_02930"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3011496.1"
                     /translation="MLLPLHDDNPLKRIRFGYFTVAIIAACVAVFIYQLSLPPDAATR
                     FVFSFGAIPSVVFGLKALPAEIAQVPATVTLVTSMFLHGSLMHLVGNMLFLWVLGDNV
                     EDALGHLRFVVFYSLSGVAAALSQTAVEPASTVPMIGASGAISGVIGAYLILHPLAPI
                     KTLVFRFIVNLPAYVVLGLWIAFQFLNASIAEPGQAGIAWWAHIGGFFAGVILIVPMR
                     QRGVPLFGRPVR"
     gene            954..2078
                     /locus_tag="AC16CX_02935"
     CDS             954..2078
                     /locus_tag="AC16CX_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011497.1"
                     /translation="MGQAAAIQAPSPADAPQVPEGTDLIEWYYERGYSDGLPLVPPTP
                     EKIAATVATLGGEPGRLLCRVPPRWGSLTREVLAINMVMAGCRPEYAPVVEAAMLALC
                     DARFNLNGVQATTHMASPLLVVNGPVRKANGMNAGCNVFGSGNRANATIGRALRLILL
                     NVGGGWPGLLDKSTLGHPGKYTYCIAENEEASPFAPYHVEKGYKAEDSTVFVLAAEPP
                     HSVTNHFANDAEGILDSVCSAMSTICNNNAVSAGHCAVVLGPEHAVTIAKSGWKRHDI
                     RHYLWAHSGNRWSDLYGNGRYGKVYNRNLPVWYKREPDARTPIVPSPDNIHLFVAGGE
                     AGRFSAFIPGWGHMSSPVLRGVGSVQPTGGLECSDGTCAV"
     gene            2242..2808
                     /locus_tag="AC16CX_02940"
     CDS             2242..2808
                     /locus_tag="AC16CX_02940"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2527..2529,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3011498.1"
                     /translation="MSTIPVYDPRGVVEAAPLATAPRVKKLQGLRLGLLDNTKWNANK
                     LLRGVRDRLAQKHAFGAVNYYRKEGFAAAAAPELLGRIAAENDVVITAIGDUGSCTSC
                     CMHDTVMLESKGRPTAAIVTTEFVLEAETQRDALGMAGLEPVVITHPLSSLTDVEIGQ
                     RIEQALPQIERVWLTGKAGGARGVKDEG"
     gene            2805..3827
                     /locus_tag="AC16CX_02945"
     CDS             2805..3827
                     /locus_tag="AC16CX_02945"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011499.1"
                     /translation="MKDEGKDVRSSPRHSLHVTHWSLGLIAAAVLSVPMVLAEAAEKY
                     PARPIRMIVPSGAGGITDILGRVMASKLGESLGQQVVVDNRPGASGIVGSSIVAKAAP
                     DGHTLLMVFPSHPVNASLFSDIPYDTIRDFAPITMVSAVSPVLIVAGQFPAKSVAELI
                     AIAKDKPGQLNHGSVGPSSMGHLGAELFRSMAGIKITFVTYKGSPQALTALLSGEIHF
                     YLIGSAGTAATHVKAGRVRALGVGARQRIAALPDVPPIADTLPGYEARGWNGILAPAG
                     TPGPVIDRLHQEIIKIVRSPEFNQVLTNEGATPIGNTPTEFDAVIRADVKKWAKIIKE
                     AGIKPQ"
     gene            3873..4667
                     /locus_tag="AC16CX_02950"
     CDS             3873..4667
                     /locus_tag="AC16CX_02950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3011500.1"
                     /translation="MPYLDLPDRKLFYTVDDHTDPWTRADTVLFVHGFTECTEAWRAW
                     VPYFSRRYRVLRIDQRGFGQSGPVPKDFPLTTELFVDDLARVIRHVADGPVHVIGGKS
                     GGISVMMLAATRPELTKTITVACSPVTPPKAEGWIEEMERNGVRSWAKRTQRERMGSR
                     MPEAGIEWWSDMMGRTALSTVHAYLRWVGAIDIREDIKRIRCPTLVIGTDTARRGRSV
                     FESWQKTIPNSELVMLPIDGYHAAGTDPDGTARVTLDFIERHSSKG"
     gene            4724..>5772
                     /locus_tag="AC16CX_02955"
     CDS             4724..>5772
                     /locus_tag="AC16CX_02955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011501.1"
                     /translation="MPAPSGFRGILLLLLSIAVAPTVFAQSYPAKPVRMVVPFAQGGC
                     PGSRRWGWCWGGDGAMPPGWWHGALQRTAIVMLALTSLVAGAAFAQQYPVKPVRLVVP
                     FAPGGGVDVTARILAQQLTQRVGQSFLVENRTGASGIIGVEYVAKSAPDGYTLLVGSQ
                     TTQAVVPAMYKVSYDTVRDFAGVTEIARSPLLIVVHPSLPVKTVADLIKLAKARPGQL
                     TFGAASGGTPHLAGELFKLTAKVDMLFVPYKGEGPAVADAMGGQISLVFSNLPVALPQ
                     ARGGKLRGIAVTSAQRVPSAPDIPTVAESGLPGFEAFTWFGFFAPGATPREIVTKLNA
                     DSVAALNTPDVKEKMA"
BASE COUNT          987 a         1916 c         1889 g          980 t
ORIGIN      
        1 ggtaacagag tgccttcata cgaacctccg tggggtggtt ggcaaggcaa atgccggcgt
       61 cctatgtttg acttttcatt gtttcaatgg ttcacctccg ctgacgacga acgaagcgaa
      121 aatttgcgca ggcatcgggg ccgagtcccg gatcccggaa agcaaattca tcattttgcc
      181 accgtcatgc ttgattgagt ccgccccgtg ctgctgccgc tgcacgacga caatccgctg
      241 aaacgcatcc ggttcggata tttcacggtc gcgatcatcg cggcgtgcgt cgcggtgttc
      301 atctatcagc tctccctgcc gcccgacgca gcgacgcggt tcgtcttttc gttcggggcc
      361 attccctcgg tcgtgttcgg cctgaaggcg ctcccggccg agatcgcgca ggtgccggcg
      421 actgtcacgc tcgtcaccag catgttcctg cacggcagcc tgatgcacct cgtcggcaac
      481 atgctgttcc tgtgggtgct cggcgacaac gttgaggacg cgctcgggca tttgcggttc
      541 gtcgtgttct attctctgtc gggcgtcgcc gccgcgttga gccagaccgc cgtcgagccg
      601 gcttcgacgg tgccgatgat cggcgcgagc ggcgcgatct cgggcgtgat cggcgcctac
      661 ctgatactgc acccgctggc gccgatcaag acgctggtct tccgcttcat cgtgaacctg
      721 ccggcgtacg tggtgctcgg gttgtggatc gctttccagt tcctgaacgc ctccatcgcc
      781 gagccaggcc aggccggcat cgcctggtgg gcgcacatcg gcggcttttt cgccggcgtg
      841 atcctgatcg tgccgatgcg ccagcggggc gtgccgctgt tcggtcggcc ggtgagataa
      901 tggggtcagg acccttttca cgccctttct ggtctttcat cgggaaacag aacatgggac
      961 aagccgccgc catccaggcg ccgtcgccgg ccgacgcgcc gcaggtgccc gagggcaccg
     1021 atctcatcga gtggtattac gagcgcggct attccgacgg cttgccgctc gtgcccccca
     1081 cgccggagaa gatcgcggcg acagttgcca cgctcggcgg cgagcccggc cgcctgcttt
     1141 gccgcgtgcc gccgcgctgg ggcagcctca cgcgcgaagt tctggcaatc aacatggtga
     1201 tggcgggctg ccggcccgaa tacgcgccag tggtcgaggc ggcgatgctc gctctgtgcg
     1261 atgcgcggtt caacctgaac ggcgtgcaag ccacgaccca catggcaagc ccgctgctgg
     1321 tggtgaacgg gcctgtccgc aaggccaacg gcatgaacgc cggctgcaac gtattcggct
     1381 cgggcaaccg cgccaatgcc acgatcggcc gcgcgctgcg cctgatcctc ctcaacgtcg
     1441 gcggcggctg gccggggctg ctcgataaga gcacgctcgg ccatcccggg aagtacactt
     1501 actgcatcgc cgagaacgag gaggcgagcc cgttcgcgcc ctatcacgtg gaaaaaggct
     1561 ataaggccga ggactccacc gtcttcgtgc tcgccgccga gccgccgcac agcgtcacca
     1621 accacttcgc caacgacgcg gaagggatcc tcgacagcgt ctgctccgca atgagcacga
     1681 tctgcaacaa caacgccgtc tcggccggac attgcgcggt ggtgctcggc cccgagcacg
     1741 cggtgaccat cgccaaatcc ggctggaaac gccacgacat ccgccattac ctgtgggcgc
     1801 attccggcaa ccgctggagc gacctctacg gcaacggccg ctacggcaag gtctacaacc
     1861 gcaacctgcc ggtgtggtac aagcgcgagc ccgatgcgcg cacgccgatc gtgccgagcc
     1921 ccgacaacat ccacctgttc gtcgccggcg gcgaagccgg gcgcttctcc gccttcatcc
     1981 ccggctgggg gcacatgtcc tcgccggttc tgcgcggcgt gggcagcgtg cagccgacgg
     2041 gcgggctgga atgctcagac ggcacttgtg ccgtctagaa cccgggagat cgggaggtcg
     2101 ggagatcggg agatcgaaaa accgagagat cgtgaatttt gctgctctcc cgcgctcacg
     2161 ccctcccgcg ctcacgccct cccgccctca cgttcagcgt gctacgcccg gagacccaac
     2221 atcaccgtcc caggaggacc tatgagcacg atccctgtct atgacccgcg cggcgtggtg
     2281 gaagcggcac ccctggcgac ggctccccgc gtgaagaagc tgcaaggcct gcggttgggc
     2341 ctgctcgaca acaccaagtg gaacgcgaac aagctgctgc gcggcgtgcg cgaccggctc
     2401 gcgcagaagc acgccttcgg cgcggtgaat tattaccgca aggaaggctt cgcggctgcc
     2461 gccgcgcccg agctgctcgg ccggatcgcc gccgagaacg acgtcgtcat caccgccatc
     2521 ggcgactgag ggtcctgcac gtcgtgctgt atgcacgaca cggtgatgct ggagtcgaag
     2581 ggccgtccca cggccgcgat cgtcacgacc gagttcgtgc tcgaggccga gacgcagcgc
     2641 gacgcgctcg gcatggcggg gctcgagccg gtcgtgatca ctcatcccct gagctcgctc
     2701 accgacgtgg aaatcgggca gcggatcgag caggcgctgc cgcagatcga gcgcgtctgg
     2761 ctcaccggca aggcgggcgg cgcccgaggg gtgaaggatg agggatgaag gatgagggaa
     2821 aagatgtgag gtcgtcaccg cgtcactcgc ttcacgtcac tcactggtca ctcgggctga
     2881 ttgcggcggc ggtgctgtct gtgccgatgg ttctggccga agccgcagag aagtaccccg
     2941 cacggcctat ccgcatgatc gtccccagcg gggcaggcgg gattaccgat attctcggcc
     3001 gcgtgatggc gtcgaaactt ggcgagagcc tcggccagca ggtcgtcgtg gacaatcgcc
     3061 ccggcgcgag cggcatcgtc ggctccagca tcgtggccaa agccgcgccg gacggccaca
     3121 cgctgctgat ggtcttcccg tcccacccgg tgaacgcgag cctgttctct gacataccgt
     3181 acgacacgat ccgcgatttc gcgccaatca cgatggtgag cgcggtgtcg ccggtgctga
     3241 tcgtggcggg ccagtttccc gcgaaatccg tggctgagct gatcgccatc gcgaaggaca
     3301 agccgggaca gctcaaccac ggctccgtgg gcccaagcag catggggcac ctcggggcgg
     3361 agctgttccg ctcgatggcc ggcatcaaga tcacgttcgt gacctacaag ggctcgccgc
     3421 aggcgttgac ggcgctcctt tccggcgaga tccacttcta cctcatcggc tcggcaggca
     3481 ccgcggcgac gcacgtgaag gccgggcgcg tgagggcgct cggcgtcggc gcaagacagc
     3541 gcatcgcggc gctgcccgac gtgccgccga tcgcggacac gcttccggga tacgaggcgc
     3601 gcggctggaa cggcatcctc gcgccggccg gcacgcccgg gccggtgatc gaccgcctgc
     3661 atcaggaaat catcaagatc gtccgctcgc cggaattcaa ccaggtgctg acgaacgaag
     3721 gggcgacgcc catcggcaat acaccgacgg aattcgacgc cgtcatccgc gcggacgtga
     3781 agaagtgggc gaagatcatc aaggaagccg gcatcaaacc ccagtaaacg ggaacttgta
     3841 gttcgtcact cgacactcga cactgacgca tcatgcccta cctcgatctc cccgaccgca
     3901 agctgttcta taccgtggac gaccacaccg atccgtggac gcgcgcggac accgtgctgt
     3961 tcgtgcacgg cttcaccgag tgcaccgagg cgtggcgcgc ctgggtgccg tatttctcgc
     4021 gccgctaccg cgtgctgcgc atcgaccagc gcggcttcgg acaatcgggg ccggtgccga
     4081 aggattttcc gctcacgacc gagctgttcg tggacgatct cgcgcgcgtc atccggcacg
     4141 tcgcggatgg tccggtgcac gtgatcggcg gtaagagcgg cggcatcagc gtcatgatgc
     4201 tcgccgcgac gcgccccgag ctcacgaaga ccatcacggt cgcctgctct cccgtcacgc
     4261 cgcccaaggc cgagggctgg atcgaggaga tggagcgcaa tggcgtgcgc agctgggcga
     4321 agcgcacgca gcgcgaacgg atgggaagcc ggatgccgga agcgggcatc gagtggtggt
     4381 ccgacatgat gggtaggacg gcgctctcga ccgtgcacgc gtatctccgc tgggtcggcg
     4441 caatcgacat ccgggaagac atcaagcgca tccgatgccc gacactcgtc atcggcaccg
     4501 acaccgcgcg cagaggcagg tcggtgttcg agagctggca gaagacgatc ccgaattccg
     4561 agctcgtgat gctgccaatc gacggctacc acgcggcggg cacggatccc gacggcaccg
     4621 cccgcgtcac gctcgatttc atcgagcggc attcgtctaa aggatgaggg aaaagaggta
     4681 aactcattgc tctttcctgt catcgctcat caggagctta accatgccgg ccccttctgg
     4741 tttccgtgga attttgctgt tgctgctctc gattgcggtg gcgccaaccg tcttcgctca
     4801 atcctacccg gccaagccgg tgcggatggt ggtgccgttc gcgcaagggg gatgccccgg
     4861 ctctcggcga tggggttggt gttggggcgg agacggggca atgccgccgg gctggtggca
     4921 cggcgctctg cagaggacgg caatcgtgat gctcgcgctg acttccctcg tcgcgggagc
     4981 agcattcgct caacaatatc cggtgaaacc agtacggtta gttgtaccgt ttgcgccagg
     5041 cggcggcgtg gacgtgaccg cgcgcatcct cgcgcagcag ctgacgcagc gcgtggggca
     5101 atcgttcctg gtcgaaaacc gcaccggcgc gagcggcatc atcggcgtcg agtacgtggc
     5161 caagtccgcg cccgacggct acacgctgct ggtcggctcc cagaccaccc aagcggtggt
     5221 gccggcgatg tacaaagtga gctacgacac ggtgcgcgat ttcgccggcg tgaccgagat
     5281 tgcgcgatcg ccgctgctga tcgtcgtgca cccctcgctg ccggtaaaga cggtcgcgga
     5341 cctgatcaag ctcgccaagg cccggcccgg ccagctcacc ttcggcgcgg cctccggcgg
     5401 cacgccgcac ctggcgggcg agctgttcaa gctgacggcc aaggtggaca tgctgttcgt
     5461 gccgtacaag ggcgaaggcc cggcagtcgc cgacgcgatg ggtggccaga tctcgctggt
     5521 gttctcgaat ctcccggtcg cgttgccgca ggcgcgcggg ggcaagctgc gcgggatcgc
     5581 ggtcaccagc gcccagcgcg ttccttcagc acccgacatc cctaccgtgg cggaatccgg
     5641 cttgccggga ttcgaagcct tcacctggtt cggctttttc gcgcccggcg caaccccgcg
     5701 cgagatcgtc acgaagctca acgccgactc ggtcgccgcg ctcaacacgc cggacgtgaa
     5761 agaaaagatg gc
//
LOCUS       DBNGWZ010000100         3023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3031483, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000100 DBNGWZ010000000
VERSION     DBNGWZ010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3031483"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="AC16CX_02960"
     CDS             <1..303
                     /locus_tag="AC16CX_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011502.1"
                     /translation="FNDSEIIAHYLAHNIKREGRTFEQALRASLDDFDGVFSYLAATE
                     AQMGAVRDRLGIKPILFYQNDGLLLLGSEQVSFEAVDPDIYATEMAPGEVRTWSKN"
     gene            288..992
                     /locus_tag="AC16CX_02965"
     CDS             288..992
                     /locus_tag="AC16CX_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR3011503.1"
                     /translation="MVEELDLEQIGEKELNARLQALVNGGRKVVLRNTHARHNIAVNV
                     SGALEIEVQGSAGFYCCGFMNGPMVTVTGNVGWYAADNMLGGKLVVLNNAGSNLAPSM
                     IGGTVLVRGSAGSRVGYGLKGGTVVVCGDVGMLTGQQMLGGRIVLLGKVGENTGESMY
                     GGVIYYRRGQLAGTGSNIRVRALEAAEAEELAVPFAENDIQADPAEFECLVPKSGKQK
                     FKLFKPHLVEREKVRP"
     gene            989..2395
                     /locus_tag="AC16CX_02970"
     CDS             989..2395
                     /locus_tag="AC16CX_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3011504.1"
                     /translation="MIICDAALCTDCGKCINVCPPKALRFPSAAEGAVTTLASSNGGG
                     LTGARLTGGGWNSSTVREIQEKARTGKHLIRGCGTTRKLPTFDDLVLLSAGLSRMPVD
                     TYREDAVTRTVLGARFAKKPLVIETPIMVAAMSYGALSKEAKIALAMGSAKVGTAMNN
                     GEGGLLPEERAHSYRQIVQVCPSRFGFSLRNLEEADAIELVFGIGAKPGLSGHLMAEK
                     MTAEIAAFRGLPAGIDLASHPRHGDIFGADDLVVKLQELRDITHSEKPIFLKIGAGRV
                     YEDVKIAAKVGVDGVVIDGMQGGTGAGPKIAIDHLGMPTLAAIVQATRALEDMGVKDE
                     VSLIVAGGVRDGADLAKALALGADAVYVGTGAMVAMGCTVCLECHTGECEFGIGTQKP
                     ELRARLNVKLAAHRVANYFTALTNEMVILAKACGKSNVHNLEREDLRAVTLEACAMTG
                     IPFIGSDYTFTEPFGFFG"
     gene            2478..>3023
                     /locus_tag="AC16CX_02975"
     CDS             2478..>3023
                     /locus_tag="AC16CX_02975"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3011505.1"
                     /translation="MCGIGGVKLAKPGPIGEELVQMMTALRHRGTDSTGFALYGPWQE
                     DRLIARLRVAERGKLEAKLNEIEAELQKAGGKFVSSPTWDKADRARDAFVRLEVSPNV
                     DVERVTAAWITLGGIEVHSFGHSLEIIKDVGDARAVAGRHGIREFQGTHGLGHCRLAT
                     ETEVDVAHGHPFWARPFPDVAI"
BASE COUNT          567 a          924 c         1041 g          491 t
ORIGIN      
        1 ttcaacgatt ccgagatcat cgcccactat ctcgcccaca acatcaagcg cgagggccgc
       61 acgttcgaac aggccctgcg cgcctcgctc gacgatttcg acggcgtgtt ctcgtacctg
      121 gccgccaccg aggcgcagat gggggcggtg cgcgaccggc tgggcatcaa gcccatcctc
      181 ttctatcaaa acgacggcct gctgctgctc ggctcggagc aggtgtcgtt cgaggcggtt
      241 gatccggata tttacgccac cgagatggcc ccaggagaag tgcgaacatg gtcgaagaac
      301 tagacttgga gcagatcggc gagaaggaat tgaatgcgcg ccttcaggcg ctcgtgaacg
      361 gagggcgcaa ggtcgtcctg cgcaatacgc acgcccgcca caacatcgcc gtcaacgtca
      421 gcggcgcgct ggaaatcgaa gtgcagggca gcgccgggtt ttactgctgc ggcttcatga
      481 acggcccaat ggtcactgtg accggcaacg tgggctggta cgccgccgac aacatgctgg
      541 gcggaaaact ggtggtgctg aacaacgccg ggagcaacct cgccccctcg atgatcggcg
      601 ggacagtgct cgtgcgcggc tcggcgggca gccgcgtggg ctacgggttg aagggcggaa
      661 ccgtggtcgt ctgcggcgac gtggggatgc tcacgggcca gcagatgctc gggggccgca
      721 tcgtcctcct cggtaaggtc ggcgagaaca cgggcgaatc catgtacggc ggcgtcatct
      781 actaccggcg tgggcaactg gccgggacgg gaagcaacat ccgcgtgcgc gcgctggagg
      841 ctgctgaagc cgaagaactg gccgtgccct tcgccgagaa cgatattcag gccgacccgg
      901 cagaattcga gtgcctcgtc ccaaaatcgg gcaagcagaa gttcaagttg ttcaagcctc
      961 acctcgtcga gcgtgagaag gtgcggccat gattatctgt gacgcggctc tgtgcacgga
     1021 ttgtggcaag tgcatcaacg tctgtccgcc caaagcgctg aggtttccca gcgctgccga
     1081 gggcgccgtg acgacgttgg cgagcagcaa tggcggcggc ctcactggcg cgcggctgac
     1141 cggcggcggg tggaattcct ccaccgtgcg cgagattcag gagaaggcgc gcacgggcaa
     1201 gcacctcatc cggggctgcg gcaccacgcg caaactgccc accttcgacg accttgtgct
     1261 gctctcggcg ggactctcgc gcatgccggt ggacacgtac cgtgaggacg ccgtgacccg
     1321 caccgtgctc ggcgcgcgct ttgcgaaaaa gccgctcgtc atcgagacgc ccatcatggt
     1381 cgcggcgatg tcgtacgggg cgttgagcaa ggaagcgaag atcgcgctgg cgatggggag
     1441 cgcgaaagtc ggcacggcga tgaacaatgg cgaaggcggc ctgctgcccg aagagcgtgc
     1501 gcactcctac cgtcagatcg tgcaggtgtg ccccagccgg ttcggattct cgctccgcaa
     1561 cctcgaagag gcggatgcga ttgagctggt cttcggcatc ggcgcgaagc ccggcctctc
     1621 cggccacctc atggccgaaa agatgacggc ggagattgcg gcctttcgcg gcctgcctgc
     1681 cgggattgac ctcgcctcgc acccgcgcca cggcgacatc ttcggggccg atgacctggt
     1741 cgtgaagttg caggagctgc gcgacatcac ccattccgag aagcccatct tcctcaaaat
     1801 cggcgcgggc cgcgtgtacg aggacgtgaa gatcgcggcg aaggtcggcg tggacggggt
     1861 ggtgattgac gggatgcagg gcggcacagg ggccgggccg aagatcgcga ttgaccatct
     1921 cggcatgccc acgctcgccg ccatcgtgca ggcgacgcgc gcgctggaag acatgggcgt
     1981 gaaagacgag gtaagcctga tcgtcgccgg cggcgtccgc gacggcgcgg accttgcgaa
     2041 ggcgctggcg ttgggcgcgg atgccgtgta cgtgggcacg ggcgcgatgg tggcgatggg
     2101 ctgcacggtc tgcctcgaat gccacacggg cgagtgcgaa tttggcatcg gcacgcagaa
     2161 gccggagctg cgcgcccggc taaacgtcaa gctcgccgcg caccgcgtcg ccaactactt
     2221 caccgcgctg accaacgaaa tggtgattct ggcgaaagcc tgcggcaaga gcaacgtcca
     2281 caacctcgag cgtgaagacc tgcgcgcggt gacgctcgag gcgtgcgcga tgacgggcat
     2341 ccccttcatc ggctccgact atactttcac cgagccgttc gggttctttg gctgatcgca
     2401 gatcgcgact cgcggcaatg tacgataggc aataagcgat atgcgatacg ctataagcga
     2461 tatgcgagga ggcggctgtg tgcggcatcg gcggtgttaa actggcaaag cctggtccca
     2521 tcggcgagga gctggtgcag atgatgacgg ctctccgcca tcgcggcacg gattcgaccg
     2581 gtttcgcgct gtacggcccg tggcaggagg atcggctgat cgctcggttg cgggtcgcgg
     2641 aacgcggaaa actcgaggcg aaattgaacg agatcgaagc cgagttgcag aaggccggag
     2701 gcaagttcgt cagctcgccc acgtgggaca aagccgaccg ggcgagagat gcgttcgtcc
     2761 ggctcgaagt gagtccgaac gttgacgtcg agcgcgtgac cgccgcgtgg atcacgctcg
     2821 gcgggatcga agtccattcc ttcggccatt cgctcgaaat catcaaagac gtgggcgatg
     2881 cgcgagcggt cgccgggcgg cacggcatcc gcgagtttca aggcacgcac gggctgggcc
     2941 actgccgcct cgccaccgaa accgaagtgg acgtggcgca cggccacccc ttctgggcgc
     3001 ggcccttccc cgacgtggcc atc
//
LOCUS       DBNGWZ010000101         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_134603, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000101 DBNGWZ010000000
VERSION     DBNGWZ010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_134603"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..1660)
                     /locus_tag="AC16CX_02980"
     CDS             complement(287..1660)
                     /locus_tag="AC16CX_02980"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR3011506.1"
                     /translation="MSRSQEPRAPNIVLILADNLGWGELGCYGGGALRGAPTPRIDKL
                     AADGLRLLNFNVESDCVPTRSALMTGRHPIRTGAIQAVPAGLPQGIIPWEITLAQLLS
                     GRGYATGIFGKWHLGDREGRYPTDRGFDEWYGIPRTTNESMFTSSPGYDPQAVPLPYV
                     MEGRKREPARNVAVYDLEMRRRIDSDLVARTNDFMRRQVKAGKPFFAYVPLTQLHYPP
                     IPHHDFAGRTGAGDFADSMAEMDHRVGQILDEMDSLRITSDSLVIFASDNGPEFRRPW
                     RGTAGPWTGTYHTAMEGGLRAPFMLRWPGRVPPGRASNEIVHVTDLYTTLARVAGANG
                     PSDRPMDGVDQLDFFLGKREKSNREGFVYYIKQELRAVKWRDWKMHLVWEAEPNAGPN
                     RLESPLIFNLIQDPKEETDVHTASSWVRTPMRRLIHEFQESLKAHPPIPPGAPDDFIP
                     QGGRARR"
     gene            1845..>2532
                     /locus_tag="AC16CX_02985"
     CDS             1845..>2532
                     /locus_tag="AC16CX_02985"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3011507.1"
                     /translation="MRVGLFVTCLVDLMRPRIGFAALKLLAAAGCDVVVPRAQTCCGQ
                     PGYNAGDRHSAAALARKVLAEFGDCDYVVAPSGSCAGMIRTHYPELFHDDPGTLERVN
                     RLCARTYELTDFLVNVARLGAVPGNLKGTITYHDSCSGLRELNVKNQPRQLLAKMPGL
                     TLKEMEEAETCCGFGGAFAVKFGEISTHIAERKCANIEACGADAVVLGDLGCMLNIEG
                     RLRRRGDMKTK"
BASE COUNT          434 a          797 c          836 g          465 t
ORIGIN      
        1 cgcttttcca tcacgaaacc gctcttgtct ctcggcgtgt ggcagccgtc gcaggccatg
       61 acggcgttga cgagatagct gccacgctca agcggtgtct gggcgattgc accgccaacc
      121 gctgcgatga acgcagccac gcacgcgatg acaaacctga ccatagtccc ccctgttact
      181 ttgcgcggcc gttgcagccg cggatggtca cagggtaatg caagcgccaa atgcgatcaa
      241 tcgcccttga agtctggaga aagcgggagg aattccacct tgccgctcaa cgtcttgccc
      301 ggccaccctg cgggatgaaa tcgtccggcg cgccgggcgg aatcggcggg tgcgccttca
      361 ggctttcctg gaactcgtgg atcaggcggc gcatcggcgt gcgcacccag ctcgatgcgg
      421 tgtgaacgtc ggtctcctcc ttggggtcct gaatcaggtt gaagatcagc ggtgattcaa
      481 ggcggttcgg tcccgcgttc ggctccgctt cccacaccag gtgcatcttc cagtcgcgcc
      541 atttgacggc gcgcagctcc tgcttgatgt aatagacgaa tccctcgcgg ttcgacttct
      601 cccgcttacc gaggaagaag tcgagctggt ccacgccgtc catcggccgg tcggatgggc
      661 cgtttgctcc ggcgacacgc gcgagtgtcg tgtagaggtc ggtcacatgg acgatttcgt
      721 tactcgcgcg gcccggcggg acacggccgg gccagcgaag catgaacggc gcgcgcagcc
      781 ctccttccat cgcggtgtga taggtgccgg tccacgggcc cgccgtgccg cgccacgggc
      841 gccggaactc ggggccgttg tcgctcgcga agattaccag cgagtcgctg gtaatcctta
      901 atgaatccat ttcatccaga atctggccga cacggtgatc catttccgcc atggagtcgg
      961 caaaatcgcc ggcgccggtc ctgccggcaa aatcgtggtg cgggatcggg gggtagtgca
     1021 gctgagtcag aggcacgtag gcgaagaacg gtttcccggc ctttacctgc cgccgcatga
     1081 agtcgttcgt ccgcgcgacg agatcggagt cgatgcgccg gcgcatctcg aggtcataga
     1141 ccgcgacgtt gcgcgcgggc tcgcgcttgc gcccttccat cacgtagggc agcggcacgg
     1201 cctgcggatc gtagccgggc gagctcgtga acatggattc attggtggtg cgcgggatgc
     1261 cgtaccactc gtcgaagccg cggtccgtcg gatagcggcc ttcgcggtcg ccgaggtgcc
     1321 atttcccgaa gatgccggtc gcgtagcccc ggccggacag gagctgcgcg agcgtgattt
     1381 cccacgggat gatgccctgc ggcaggccgg ccggtaccgc ctgtatcgcc ccggtgcgga
     1441 tcggatgccg gcccgtcatc aaggcggagc gtgtcggcac gcaatcgctt tcgacgttga
     1501 agttgagcag gcgaagtccg tcggctgcga gcttgtcaat acgcggcgtc ggcgcgccgc
     1561 gcagcgcccc gccgccgtag cagccgagtt cgccccatcc gaggttgtcg gcgaggatca
     1621 ggacgatatt gggggcgcgc ggctcttgac ttcgcgacat gcgtgccatc cagcgagatc
     1681 agcggattgc atcctagcac agcgatgtcc tccgaccggc acgggggtcc gcagagggat
     1741 ttctgcgata atgacccgtt caataagagg tgattcgcgc gaagcgcggc ggaccagtca
     1801 ccagtcacca gtcaccagtc acgagtcacg cttctcggaa gaaaatgcgc gtcggccttt
     1861 tcgtcacctg cctcgtggat ctcatgcggc cgcgaatcgg gttcgcggcg ctgaagttgc
     1921 tcgcagccgc cggctgcgac gtggtggtgc cgcgcgcgca gacctgctgc ggccagccgg
     1981 ggtataacgc cggcgaccgg cactcggcgg ctgccctcgc gcgcaaggtg ctcgccgagt
     2041 tcggggattg cgactacgtg gtggcgccct ccggctcgtg cgccggcatg atccggacgc
     2101 attatccgga gctgtttcac gacgatcccg gcacgctgga gcgcgtgaat cgactgtgcg
     2161 cccgcacgta tgaattgaca gatttcctgg tgaacgtcgc caggctaggc gccgtcccgg
     2221 gaaatcttaa aggaaccatc acctatcatg actcctgctc cggcttgcga gaattgaatg
     2281 ttaagaacca gccgcgtcag ctactcgcca aaatgccggg attgacgctg aaggagatgg
     2341 aggaggccga aacctgctgc ggcttcggcg gcgcgttcgc ggtcaagttc ggtgagatct
     2401 ccacccacat cgccgagcgc aagtgcgcca acatcgaggc atgcggcgcg gacgcggtgg
     2461 ttctgggcga tctcggttgc atgctcaaca tcgaaggccg cctgcgccgc cgcggcgaca
     2521 tgaagacgaa gg
//
LOCUS       DBNGWZ010000102         1862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3409883, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000102 DBNGWZ010000000
VERSION     DBNGWZ010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3409883"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..858)
                     /locus_tag="AC16CX_02990"
     CDS             complement(25..858)
                     /locus_tag="AC16CX_02990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3011508.1"
                     /translation="MNAMNRGYSIMRAALLVVGAVALASCKTVQTTQPGTVGVERKQS
                     MLLSSAEVDKAAGQAYQQTLQQSSKKGALNRDAAQVQRVRAIANRLIPATGAFRPDAP
                     SWKWEVNVITSPEINAWCMPGGKIAVYTGLIEKMRVTDDELAAVMGHEIAHALREHGR
                     ERASQQMAQNAVIGIGAALLGIGDAGAQLVNVVADVTVSLPYSREFEREADRIGVELA
                     ARAGYDPHAAVTLWQKMAQSGGGAPPQFLSTHPSPESRIQDLQAYSARVMPLYESARK
                     R"
     gene            complement(1205..1525)
                     /locus_tag="AC16CX_02995"
     CDS             complement(1205..1525)
                     /locus_tag="AC16CX_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR3011509.1"
                     /translation="MSDWVTVAKVDELAPGQFRNVDVDGASVVVFNLGGEYYAIEDVC
                     THDGGQLTGGIVDGDQIECPRHGARFCIRTGEALTAPAYEPVPKFPVRVENGVIQVRD
                     DRWD"
     gene            1591..1713
                     /locus_tag="AC16CX_03000"
     CDS             1591..1713
                     /locus_tag="AC16CX_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011510.1"
                     /translation="MNKIPNTGKPKEKLHGAASLAVQLLFLFLLAALAVQFFFF"
BASE COUNT          326 a          592 c          564 g          380 t
ORIGIN      
        1 ggtttgatct agatcaatcc gggatcagcg cttgcgcgcg gattcgtaga ggggcatcac
       61 gcgcgccgag tacgcctgaa gatcctggat ccggctctcg ggtgaggggt gcgtggacaa
      121 gaattggggc ggggcgccgc cgccggactg cgccatcttc tgccacagcg tgaccgcggc
      181 gtgcgggtca tagccggcgc gcgcggcgag ctccacgccg atgcggtcgg cttcgcgctc
      241 gaattcgcgc gaataaggca ggctgaccgt gacgtccgcg acgacgttca cgagctgcgc
      301 gccggcgtct ccgatcccga gcagcgcggc gccgatgccg atcacggcgt tctgcgccat
      361 ctgctgcgag gcgcgctcgc ggccatgctc gcgcagcgca tgcgcgatct catggcccat
      421 caccgcggcg agctcgtcgt cggtcacgcg catcttctcg ataagcccgg tgtagaccgc
      481 gatcttgccg ccgggcatgc accacgcgtt gatctccggc gaggtgatca cgttgacttc
      541 ccatttccac gagggagcgt ctgggcggaa cgcgccggtc gcgggtatca gccggttggc
      601 gatggcgcgc acgcgctgca cctgcgccgc gtcgcggttg agggcgccct tcttggacga
      661 ctgctgcagc gtctgctggt aagcctgccc ggcggccttg tccacctcgg ccgaggacag
      721 cagcatcgac tgcttgcgct ccacgcccac cgtccccggc tgggtggtct gcaccgtctt
      781 gcacgaggcg agtgccactg ctccgacaac cagcagcgcc gctcgcatga tggaatatcc
      841 tctgttcatg gcgttcatct taacgaaaga tcaatgcccc tggttgaact gttactgtaa
      901 ccaattgtta atcagctttt gattctccct atgcctcggt ggttcaattc tgctttgttt
      961 tccttggcgg ttctggcgtc cgtggatcga gtccgggaca agcttggcgg ctatttttca
     1021 gcggaccggc accgtcgccc gcacgagtag cgcgccggac agcgcgtggg taaacgtgtc
     1081 catggttgga gtgactaagt gactaagtga ctaagtgatt aagtgactga gtaacgagtg
     1141 agcgaagcgt cgacgggttg ggcacgtaat cacttaatca cctaatcact taatcacccg
     1201 ctcctcagtc ccagcggtcg tcccgcacct ggattacgcc gttctcgacc cgcaccggga
     1261 acttcgggac cggctcgtag gccggcgcag tgagcgcttc cccggtcctg atgcagaagc
     1321 gtgccccatg gcgcgggcat tcgatttgat cgccatccac gatcccgccg gtcaactgcc
     1381 cgccgtcgtg ggtgcacacg tcctcgatag cgtaatactc gccgccgaga ttgaacacca
     1441 cgacgctcgc gccatccacg tcaacgttgc ggaactgccc gggcgcgagc tcgtccacct
     1501 tggctacagt gacccagtca gacacggcgc aaaccccgaa ccgcggagca agacaaattc
     1561 aaatcgaacc gcagagacgc agagaggagc atgaataaga tcccgaacac cggaaaaccc
     1621 aaggagaagc tacatggagc ggcatcgctg gcggttcaac tattgttctt gttcctctta
     1681 gcggccttgg cggttcaatt tttctttttt tgattctctc cgaatctctg cggttcaatt
     1741 attctggtct tcttggcggt tcgctgttac accgcgacga cttcgatatc cggctcgatc
     1801 ggccgcagca ggctctcgat gccggccagc gtccccgaga tcgagctcgg gcagctgccg
     1861 ca
//
LOCUS       DBNGWZ010000103        13172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1268845, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000103 DBNGWZ010000000
VERSION     DBNGWZ010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1268845"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="AC16CX_03005"
     CDS             <1..168
                     /locus_tag="AC16CX_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3011511.1"
                     /translation="ETPAGDAAAYAPLAAQAQKLLSPAEMGELFKVIALGRGVTQPLV
                     GFMKGDKRHTL"
     gene            311..1081
                     /locus_tag="AC16CX_03010"
     CDS             311..1081
                     /locus_tag="AC16CX_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3011512.1"
                     /translation="MNEIALTWWVWAVIYAAMLAAGLMQGMFGFGFPIIATPLTVLVT
                     DVKTAIVLNMLPTLTLNLVSIARGGNWGESLGVYWRVAVYVALGSFLGAQVVIFAPVE
                     PLRLLLAAMIFAYLCQERLARLDWSWLSRHRRTSEAVFGMTGGFFSGSVNNSLPVLLI
                     YFMLLGVSLTVMTQVMNFCFFFGRAVQAVTLGAAGEISVASALANIPLTLVAIGGYIV
                     GARIQRLFSAATFNRLLKRIVFVMGVMLTVQGLAWFVR"
     gene            1162..1869
                     /locus_tag="AC16CX_03015"
     CDS             1162..1869
                     /locus_tag="AC16CX_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011513.1"
                     /translation="MTVPRFVCLSIAVLACTVGLGPATAADSVPSDSPGVARAQYLWS
                     QSPHGKLLERILPPSIEPGELPEPRSEGARLTARYCVQCHYLPSPQMHTADKWTIIVV
                     RMVWRMQGKGNLGVVMKEMMDNVEAPTDPEIAVLTRYLQKHAQKEMDPRHPALKSETG
                     KIYDIACTQCHALPDPRRHTAREWPEVVERMKRHMAWANTVVGPDSLKTVPVLDTAEI
                     VRFLQRYARREPVSGKQ"
     gene            complement(2052..2702)
                     /locus_tag="AC16CX_03020"
     CDS             complement(2052..2702)
                     /locus_tag="AC16CX_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3011514.1"
                     /translation="MPDNLKTIPLFSGLQDNDLRALDNKAVVRTFRKHTIIVNEGDLT
                     DSLYLILSGRVKVYLSDKSGKELILDIKGPGEYFGEMVLDEGPRSASVMTLEPSQFAI
                     IARNDFRDFILKHPEIALHIIQNLIKLARGLNESVRSLAMLDTYGRVARMLLDLATEK
                     EGKLVIPARMTQKEMASRVGASREMINRILRDLTVGGYIKVEEGFITINKPPPSKW"
     gene            complement(2838..3302)
                     /locus_tag="AC16CX_03025"
     CDS             complement(2838..3302)
                     /locus_tag="AC16CX_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011515.1"
                     /translation="MEKMLSRRDYSLIVDAEARVGYCIATSLLSYEKYTLAQSIARPN
                     GSELYSIVLSYSLAGEVTAQKQGAIRALKERDELRRRATQIAIRIADDCGYDRSALPA
                     DVSPWKITERWLNHDDGFSFFLNRANQSVPDEAEQIYRLASRKAGNPAKRKS"
     gene            complement(3708..3836)
                     /locus_tag="AC16CX_03030"
     CDS             complement(3708..3836)
                     /locus_tag="AC16CX_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011516.1"
                     /translation="MAVFGYNLLMWLCNLFITHHSSPVIGHDIRTAAPVAPRSVWP"
     gene            3975..4958
                     /locus_tag="AC16CX_03035"
     CDS             3975..4958
                     /locus_tag="AC16CX_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3011517.1"
                     /translation="MRAMQLTAPRTALRLADLPVPRPGPGQILIKVRACGVCRTDLHV
                     VDGDLTQGKLPIVPGHEIVGTVAEKGAGVERFASGERVGVPWLGYTCGACRYCVSGRE
                     NLCDGARFTGYHLDGGYAEYTVADQRYCFAIPKEYDDTAAAPLLCAGLIGYRSLTMAG
                     DAERLGVYGFGAAAHIVAQVARHQGRRIFAFSRPGDAAAQRFALKLGAEWAGDSTAKP
                     PEELDAAIIFAPVGSLVTAALRATAKGGTVVCAGIHMSEIPAFPYDILWGERVVRSVA
                     NLTRRDGEEFLKIAPQVPVRTAIETFPLGQANDALARLREGRVTGAAVLVP"
     gene            complement(5076..5810)
                     /locus_tag="AC16CX_03040"
     CDS             complement(5076..5810)
                     /locus_tag="AC16CX_03040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3011518.1"
                     /translation="MAIEERKSPCYPIPLSSLDTQSSTLVSRPSSLDTQSSARTPPRR
                     GFCRRAIFGAIVAAGCVAGAGYAQQPIVAPPALRAPDVRYEPTAMDVVQVMLRLANVR
                     AGDVVFDLGCGDGRIVIAAVRRFGARGVCVDIDPRRIAESRENAGEAGVTDRIQFLNQ
                     DLLATDISNATVVMLFLSPELNLKVRPKLLRELKAGTRVVSHWHDMGDWKPQETVRVR
                     SGGYERPVYLWIVADADKTKAVNSER"
     gene            complement(5867..7993)
                     /locus_tag="AC16CX_03045"
     CDS             complement(5867..7993)
                     /locus_tag="AC16CX_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011519.1"
                     /translation="MKTKGSLYWSVAAVGIAALLAACAAQMGSESGVSVGANDLGGVV
                     ASANGPEAGVWVIAETTELPTKFAKIVVTDDRGRFLMPDLPKATYSVWVRGYGLVDSP
                     KVRTAPGATLNLKAVPAPSPAAAAQYYPAIYWYSMLKIPAKNEFPVGKVPHQGEWLHV
                     LKTGGCVGCHSMGSLGTRTMPKDFAHMKSSDAWARRIQSGQALPQMARDINRLDAPRA
                     LALFGDWTDRIAAGELPFAKPERPQGIERNLVLTLWEWSTPTGYMHDLIGTDRRNPKV
                     NANGKFYGSAEESTDFIPILDPNTHTASAVKHPVRDPKTPSSKTSPMQPSPYWGDKPI
                     WDSQTSNHNPIMDEKGRPWFAARVRHPDNPAFCKKGSNHPSAKAFPLEQSGRHLSMYD
                     PATGKFTLISTCFPTHHLIFAEDANHTVWTSSVFGTGVVGWLNRKMFEETGDEARSQG
                     WTPLILDTNGNGKRDAYADHNQPLDPTKDKRVLLNLYAVAVNPVDGTVWGTAWFSPFP
                     GYIVRLNPGPNPTETALAEVYEPPFPGYGPRGGDIDRNGVYWASLSSGHLGKFDRSKC
                     KGPLNGPKATGKHCPEGWTLYPLPGPQFRGIEDGGSAEASYYTWVDQYNTLGLGANVP
                     IVTGNLNESYLALVNGKFVNLRVPYPQGFYAKWGEGRIDDPNAGWKGRALWATESTRT
                     VFHNEGGTANRPRVVRFQLRPDPLAR"
     gene            complement(8132..9319)
                     /locus_tag="AC16CX_03050"
     CDS             complement(8132..9319)
                     /locus_tag="AC16CX_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011520.1"
                     /translation="MLPLSGVRVLAVEQYGAGPFGTMFLADQGAEVIKIENPNDGGDM
                     SRAVGPHFFAPGDSEFFHSFNRNKKSLTLDLGRPEGQAVLHDLVHTADALASNLRGDV
                     PAKLGLTYDRLKAHNPKIVCAHLSAYGRSGPRADWPGFDYLMQAEAGYFSLTGEPGAP
                     PARFGLSIVDLMTGLGLAYALLAALTSARTSGTGRDIDVSLFDMALHNTAYLATWYLN
                     EGVITGRLPRSAHPALTPCQLYRTRDGWIYIMCNKEKFWPALCEKLGRPEWSDDARFR
                     MFPDRLRHRDLLTELLDRELERHTTREWLERFAGAVPAAPINDLAQALENPFVTEHGR
                     LQELPHAVRGSYRIVAPPVRCAGEEAPARPAPALGEHTEALLRELGYDGARIRALRDT
                     KVI"
     gene            complement(9555..10526)
                     /locus_tag="AC16CX_03055"
     CDS             complement(9555..10526)
                     /locus_tag="AC16CX_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011521.1"
                     /translation="MRKQACALAAVAAICVVTPMQAAAQQWPDKPVRILVPFPPGGGT
                     DIQARLLSSAFQKSTGQNFIIDNRTGAGGLIAGQLAVDSPPDGYTILFTSASLSVAVT
                     LYAKRMKFNALTDLAPISWISSTPLVLSVHPSVPVKSVKELVALAKRTSGNMNAAGNT
                     AGSTSHLTGEMLNQVAGIKSTVLTYRGGGPAVLALISGEVDYLFATAPSIMPHLRTGK
                     AKALAVTTPKRSSALPDLPTMNSVYPGFESDNWYAMYFPKGTPRPIVDRMNSEIRKAL
                     DSPEIREFMPREALDPVASSPEELSALLKREIEKYAKVIRQADIRIE"
     gene            complement(10819..11988)
                     /locus_tag="AC16CX_03060"
     CDS             complement(10819..11988)
                     /locus_tag="AC16CX_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011522.1"
                     /translation="MVAAPENLANEDQERLILDAVDRFLERDVRPVVHALEAADTYPA
                     AIVEKLKTLGLFGATIAPEHGGLGLTAVTYARIVERMAAVWMSVPGFFNSHLIMAAAV
                     ERYGTAEQKRRFLPRFSSGELRGGLALTEPDCGTDLQAIRTHARREGDHYVVNGAKTW
                     ITNSAEGQILAVLVKTDPWASPPHKGMSLLLIEKGPGFKVVRKLEKLGYRGIDTAELL
                     FEDCRVPAANRVGTEEGRGLQQVLNGLELGRINVAARGAGLARACLEESLRYAQQRKT
                     FGKPIAEHQAIQIKLADMATRVEAARLLTEAAARAYDAGGRCDLEAGMAKLYATEAAL
                     ENSLEAMRIHGAYGYSKEFNIERYYRDAPLLAIGEGTNELQRIIIARELVKRNPA"
     gene            complement(12195..12713)
                     /locus_tag="AC16CX_03065"
     CDS             complement(12195..12713)
                     /locus_tag="AC16CX_03065"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009915588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3011523.1"
                     /translation="MTVERKKVGPNRYRESFGRYLEDFKVGDVYEHRPGRTISEADNT
                     WFTLLTMNTNPLHFDAAYSAKTEFGRPLVDSCLTLAMVTGMSVSDVSQKAVANLGWDK
                     VRLTAPVFAGDTIYAESEVLEVRESKSRPTQGIVTVRTTGKKADGTAFMSFERTILVP
                     KRGHAVDDDVGY"
     gene            complement(12797..>13172)
                     /locus_tag="AC16CX_03070"
     CDS             complement(12797..>13172)
                     /locus_tag="AC16CX_03070"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011524.1"
                     /translation="WDDYARHLGDPATLALCKRVHTRVDPKAQADFPAEMSGSVRIKT
                     ARGAFETYVRVPKGEPANFLSAAELRAKFDGLAGPYLSGRRRDELADALAALEQAKDV
                     GAILRLTRPEQASGRRAAAGRS"
BASE COUNT         2297 a         4196 c         4078 g         2601 t
ORIGIN      
        1 gaaaccccgg caggcgacgc cgcggcctat gcgccgctcg cggcgcaggc gcagaaactt
       61 ctctctcccg ccgagatggg cgagctgttc aaggtgatcg cgctgggccg cggcgtgacc
      121 cagccgctgg tcggcttcat gaagggcgac aaacggcaca cgctgtaacg acgagtaact
      181 aagtgactgg gtgactaggt gattaagtgc atggcaacca aagtccggct ttacccagtc
      241 acttagtcac tcagtcactc aatcacgtag tcaccatcca tcactcggct tccgaggcaa
      301 gctccaggtc atgaatgaga tcgccctgac ttggtgggtg tgggccgtga tctacgcggc
      361 gatgctcgcc gccggcctga tgcaaggcat gttcggcttc ggctttccca tcatcgcgac
      421 gccgctcacg gtgctcgtga ccgacgtcaa gacagcgatc gtgctcaaca tgctgccgac
      481 gcttacgctc aacctcgtca gcatcgcccg cggcggcaat tggggagaaa gcctgggcgt
      541 gtactggcgc gtcgcggtct acgtggccct cggctccttt ctcggcgcgc aggtggtgat
      601 cttcgcgccg gtggagccgt tgaggctgct gctcgccgcg atgattttcg cctatctctg
      661 ccaggagcgg ctcgcgcggc tcgactggag ctggctttcc cgccatcggc ggacctccga
      721 ggcagtcttc ggcatgaccg gcgggttttt ctcgggcagc gtcaacaatt cgctgccggt
      781 gctgctcatc tatttcatgc tgctcggcgt ctcgctcacc gtcatgacgc aggtcatgaa
      841 cttctgtttc ttcttcggcc gcgccgtgca ggcagtcaca ttgggcgctg ccggcgagat
      901 cagcgtcgcc agcgcgctcg ccaacatccc gctcacgctg gtggcgatcg gcgggtacat
      961 cgtgggcgcg cgcatccagc ggcttttttc cgcggcgacc ttcaaccgcc tgctcaagcg
     1021 catcgtcttc gtcatgggcg tgatgctgac cgtgcagggg ctggcgtggt tcgtgcgctg
     1081 atccgaattt ctgggctaag atgaatccat atctcaaaga ccgggaatgg gcacgatcct
     1141 gatctcgaag cagaaattcg gatgactgtc ccgcgatttg tctgtttgtc cattgccgtt
     1201 ctggcctgca ctgtgggtct cggtccagcg actgccgccg atagcgttcc atccgactcg
     1261 ccgggggtgg cgcgcgccca gtacctgtgg tcgcagagcc cgcacggcaa gctgttggag
     1321 cgcatcctgc cgccctcgat cgagcccggc gagctgcccg agccgcgctc ggagggcgcg
     1381 cggctcaccg cccgttattg cgtgcaatgc cactacctgc cgagcccgca gatgcacacc
     1441 gccgacaagt ggaccatcat cgtcgtgcgc atggtctggc gcatgcaggg caagggcaat
     1501 ctgggcgtgg tgatgaagga aatgatggat aacgtggagg cgccgaccga tcctgaaatc
     1561 gcggtgctca cccgctatct gcagaaacac gcgcagaagg agatggatcc ccgccaccct
     1621 gctctcaagt ccgagacggg caagatctac gacatcgcct gcacgcagtg ccacgcgctg
     1681 ccggacccgc ggcgccatac ggcgcgcgaa tggccggagg tggtcgagcg catgaagcgg
     1741 cacatggcgt gggcgaacac ggtagtcggg cccgattcgc tcaagaccgt gccggtgctc
     1801 gacacggcgg aaatcgtgcg tttcctgcag cgctacgccc ggcgggaacc cgtaagcggt
     1861 aagcagtgag cggcaagcag aaaaagcatg gaacgcacgg cttaccgctt gcggctcacc
     1921 gctcacggcc gttcatgaaa gcaatgcagc tcgtggcgca cggcgctggt ttgattccgc
     1981 tcattcgaac aatcaatcgg gtctcagtga atatttcgcg ccaatttccg cggcgttccg
     2041 cacatgcgcg gctaccattt ggaaggaggg ggcttattga ttgttataaa tccttcttca
     2101 accttgatgt atccgccaac ggtgagatcg cggagtattc gattgatcat ctcccttgag
     2161 gcccccaccc tgctggccat ctccttctgc gtcatcctcg ccggaatgac gagcttccct
     2221 tccttctcgg tggcaagatc gagcagcatg cgcgccactc tgccataggt gtcgagcatg
     2281 gcaagacttc gcacgctctc gttcaaccct cgcgccagct tgatcaggtt ttgaatgata
     2341 tgcagcgcga tttccggatg cttgagtatg aagtccctga aatcgtttct cgcaataatc
     2401 gcgaactggg acggttcgag cgtcatcacc gatgcggacc ggggtccttc atcgagcacc
     2461 atctcaccga aatattcccc gggccccttg atatcgagaa taagttcttt gccgctcttg
     2521 tcactgaggt acaccttcac ccgaccggaa agaatcaggt agagcgagtc ggtaagatct
     2581 ccttcgttaa cgatgatggt gtgcttgcgg aatgttctga ccaccgcctt attgtcaagc
     2641 gcacgcaggt cgttatcctg caagccggag aaaaggggga ttgtttttag attgtcaggc
     2701 atggaagagc caagatcttc agggcaattt cacgataccg atccatcgtc gtcacacgcg
     2761 atcctaaccg ccactctcag tccagactgg atttcccgat cagcttggca ctgaaaaatc
     2821 cggtcatctc gcctgcgtta gcttttccgc ttcgctggat ttcctgcttt ccgtgacgcc
     2881 agtcggtaga tttgttcagc ctcatcggga accgattggt tcgctctgtt caggaagaac
     2941 gaaaacccat cgtcatgatt cagccatcgt tccgtgattt tccagggtga tacgtcggct
     3001 ggcaacgcac tccggtcata gccgcaatcg tctgctatac gaattgctat ctgcgttgcg
     3061 cgccgtcgca gctcatcgcg ctctttcaat gcccgaatgg cgccttgctt ctgcgcggtt
     3121 acctcgccgg caagcgagta ggacagcacg atcgaataca attcagaacc gttcgggcgc
     3181 gcaatgcttt gcgcgagagt atatttttca taagacaaca ggctggtggc gatgcagtag
     3241 ccgacgcgcg cttctgcgtc gacgattaac gaatagtccc tacgagacag cattttctcc
     3301 acgtaataca gccccgagta cgccaagaaa actgtcagaa gacatgagag taagagcttg
     3361 tacctcggct ggtacttgcc gggttgagcc gacatttcta tcaatcaacg acgctgatcc
     3421 ggtctgaaag aacccagatg ctgtcgaccg atcgattaat gggttcgata cgattagggg
     3481 tattcgagac tttttccacg tcacagtagt ggacactcca cgtaacctgg tgtcaacgtg
     3541 aattaagtca ctaaacaatt gtgattaaaa tcacacaatc ccatagtcat aggccgaccg
     3601 catcaatcgg aaaatcattt ggttaggaga ttggacgagc ggcctggaat ccctggcgag
     3661 agaaatgatt cccaatctgt tgcggctcgt tgaggcgaat cggcgcttca cggccagacg
     3721 gaacgcggtg ccaccggtgc cgcggtgcgg atgtcatgac cgatgacggg tgacgagtga
     3781 tgggtgatga acaagttgca cagccacatc aacaggttgt atccgaacac cgccatttca
     3841 tgcgacttat ccgaaaagct cctgatccgg tcgtagtccc gctgtgcggc acacgcaatt
     3901 gcgatttgca attgattttt tgactcgtta cttcatccac caagtgacaa tccaatttca
     3961 ggctgactgc tagcatgaga gcaatgcaac tcaccgcgcc gcgcacggcg ctcaggctcg
     4021 ccgacctgcc cgtgccgcgg ccggggccag gccagattct catcaaggtc cgcgcctgcg
     4081 gcgtctgccg caccgaccta cacgtcgtgg acggcgacct cacgcagggg aaactgccga
     4141 tcgtgcctgg ccacgagatc gtcggcacgg tggcggagaa aggcgcgggc gtggaacgct
     4201 ttgcgagcgg cgagcgggtg ggcgtgccct ggctcggcta tacctgcggc gcctgccgct
     4261 actgcgtcag cgggcgcgag aatctgtgcg acggggcgcg cttcaccggc tatcacctcg
     4321 acggcggcta cgcggaatac accgttgccg accagcgtta ctgcttcgcc attccgaagg
     4381 aatacgacga caccgcggcc gcgccgctgc tctgcgccgg gctgatcggc taccgctcgc
     4441 tcacgatggc gggcgacgcc gagcggctcg gcgtctacgg cttcggcgcg gcggcgcaca
     4501 tcgtggcgca ggtcgcgcgc caccagggac gcaggatctt cgccttcagc aggccgggcg
     4561 acgccgcggc gcaacgcttc gcgctcaagt tgggagcgga atgggcgggg gactcgacgg
     4621 caaagccgcc ggaggagctc gacgcggcga ttattttcgc cccggtgggt tcactcgtga
     4681 ccgccgcgct ccgcgccacc gccaagggcg gcacggtggt atgcgccggc atccacatga
     4741 gcgagatccc ggcgttcccc tacgacatcc tgtggggcga gcgcgtcgtg cgctcggtcg
     4801 cgaacctcac gcgccgcgac ggcgaggaat tcctgaaaat cgctccgcag gtcccggtgc
     4861 gcaccgcgat cgagacgttc ccgctcgggc aggcgaatga cgcgctcgcg cggctgcgcg
     4921 aagggcgcgt caccggcgcc gcggtgctgg tgccgtgatg aggtggatcc aaggatgaag
     4981 gcggaaagat gaaagggctg actcagtaag ttaaaaggag ttgtttcgcg gctcacgaat
     5041 cacgattcac gggtcattgg ccttctgcct tccggtcacc gttcactgtt cacggctttt
     5101 gtcttgtcgg cgtccgcgac gatccagaga taaacgggac gctcatagcc gccgctccgg
     5161 acgcgcacgg tctcttgcgg cttccagtcg cccatgtcgt gccagtgcga gacgacgcgg
     5221 gtgccagcct tcagctcgcg cagcagcttc gggcgcacct tgaggttcag ctcgggcgag
     5281 agaaagagca tgaccaccgt tgcattgctg atgtcggtcg cgaggaggtc ctggttcaga
     5341 aactggatgc gatcggttac gcccgcctcg cctgcattct cccggctttc ggcgatgcgc
     5401 cggggatcaa tgtccacgca gacgccgcgc gcgccgaacc ggcgcaccgc cgcgatgacg
     5461 atgcgcccgt cgccgcagcc gagatcgaac accacgtcgc cggcgcgcac attggcgagc
     5521 cgtaacatga cctgcaccac gtccatcgcc gtgggctcgt aacgaacatc cggcgcgcgc
     5581 agcgccggcg gcgcgacgat tggctgctgc gcatagccgg cgcccgcgac acagcccgcg
     5641 gcgacgatcg cgccgaagat cgcccggcga caaaaacccc gccgaggcgg ggtccgtgcc
     5701 gaggattgag tgtcgaggga cgagggacga gaaacgaggg tcgaggattg agtgtcgagg
     5761 gaagagagag gaatggggta acagggcgat tttcgctcct caatcgccac ccaacactcg
     5821 acacccggca ccccacactc gccactcgac acccgacact cgtcactcaa cgcgcaagcg
     5881 gatcgggccg cagctggaat cgcaccaccc tgggccggtt cgccgtgccg ccttcattgt
     5941 ggaacaccgt ccgcgtggat tccgtcgccc atagcgctct gcctttccat ccggcgttcg
     6001 gatcgtcgat gcgcccctcg ccccatttcg cgtagaagcc ctgcgggtac gggacgcgca
     6061 ggttgacgaa tttcccgttc accagggcca agtacgattc gttgaggttg ccggtgacga
     6121 taggcacgtt cgcgcccagc ccgagggtgt tgtactggtc cacccaggtg taatagctcg
     6181 cctcggcgct gccgccgtcc tctatgcccc tgaactgcgg acccggcagc ggatagagcg
     6241 tccagccctc gggacagtgc ttgccggtcg ccttcgggcc gttcaacggt cccttgcact
     6301 tgctgcggtc gaacttcccg agatgcccgc tcgagagcga cgcccagtag acgccgttgc
     6361 gatcgatgtc gccgccgcgc ggcccgtagc cggggaacgg cggctcatac acctcggcca
     6421 acgcggtctc ggtcggattc ggccccggat tgagccggac gatataaccc gggaacgggg
     6481 aaaaccacgc cgttccccac accgtcccat cgaccgggtt cacggcgacg gcgtagaggt
     6541 tcagcaggac ccgcttgtcc ttggttggat cgagcggctg gttgtggtcg gcataggcgt
     6601 cgcgtttgcc gttgccgttg gtgtcgagga tcagcggcgt ccagccctgc gagcgcgcct
     6661 cgtcgccggt ttcctcgaac atcttgcgat tcagccagcc gaccacgccc gtgccgaaca
     6721 cgctgctggt ccacaccgta tggttggcgt cctccgcgaa gatcaggtga tgcgtgggga
     6781 agcaggtgct gatcagcgtg aacttgcccg ttgccggatc gtacatcgag agatggcggc
     6841 cggattgttc gagcgggaac gccttcgccg acggatggtt cgaacctttc ttgcagaatg
     6901 ccgggttatc cggatgacgc acccgcgcgg caaaccaggg gcgccccttt tcgtccatta
     6961 tcgggttgtg attgctggtc tggctgtccc agatgggctt gtctccccaa tagggggacg
     7021 gctgcatcgg gctcgtcttc gaggacggcg tcttcgggtc gcgcaccggg tgcttcaccg
     7081 cgctcgccgt atgcgtattc gggtcgagta tcgggatgaa atccgtgctt tcctccgcgg
     7141 agccgtagaa cttgccgttg gcattgacct tggggttgcg ccggtcggtc ccgatcagat
     7201 cgtgcatata gccggtgggg gtgctccact cccacagcgt gagcacgagg ttgcgctcga
     7261 ttccctgcgg gcgctccggc ttcgcgaacg gcagctcgcc cgcggcgatg cgatcggtcc
     7321 agtcgccgaa cagggcgagc gcgcgtggcg catcgagccg gttgatgtcg cgggccattt
     7381 gcggcagggc ttgcccggac tggatgcgcc gcgcccacgc gtcggaagac ttcatgtgcg
     7441 cgaaatcctt gggcatcgtg cgcgttccca gcgagcccat cgagtggcat ccgacgcagc
     7501 caccggtctt caggacgtga agccactcgc cctgatgcgg aaccttcccc acggggaact
     7561 cgttcttcgc cgggatcttg agcatcgagt accagtagat cgccggatag tattgcgccg
     7621 cagcagccgg gctcggcgcc ggcaccgcct tcagattgag cgtcgcaccg ggcgcggttc
     7681 gaaccttggg cgaatccacg agcccgtagc cgcgcaccca cacgctgtag gtggctttcg
     7741 gaagatccgg catgaggaaa cgcccgcgat catcggtaac cacgatcttg gcgaacttgg
     7801 tcggaagctc tgtcgtctcc gcgatcaccc agacgccggc ttccggtccg ttcgcgctcg
     7861 cgaccacgcc gcccaagtcg ttcgcgccga cgctgacgcc cgactctgag cccatctgcg
     7921 ccgcgcaagc ggccagcaat gcggcgatgc cgaccgccgc cacgctccag tacaaactcc
     7981 ctttggtctt catatccccg ctcctctcat tcaagacgaa gtgacgtagt gatgaagcgg
     8041 cgtgctgaca gaatcttctg ccttcggtta ccgcccaccc actactcacc actcactact
     8101 cacgcttttg gcctggatag tttacgcctc atcaaatcac tttcgtatcc ctcaacgccc
     8161 ggatgcgcgc gccgtcatag ccgagctccc gcagcagcgc ctcggtgtgc tcgccgagcg
     8221 ccggcgccgg gcgcgccggc gcctcctcgc ccgcgcatcg cacgggcggg gcgactatgc
     8281 ggtaggaccc cctcaccgca tgcggaagct cttgcaaccg gccgtgctcc gtcacgaacg
     8341 gattctcgag cgcctgtgca agatcgttga tcggcgccgc cggcacggcg ccggcgaatc
     8401 gttcgagcca ctctctcgtc gtgtgccgct ccagctcccg gtcgagcagt tcagtcagca
     8461 agtcgcggtg ccgcagccgg tccggaaaca tgcgaaagcg cgcgtcgtcg ctccactcgg
     8521 ggcgcccgag cttctcgcac agcgccggcc agaacttctc cttgttgcac atgatgtaga
     8581 tccaaccatc gcgcgtgcga tagagctggc acggcgtcaa tgccggatgc gccgagcgcg
     8641 gcaggcgccc ggtgatgacg ccctcattga ggtaccaggt ggcgaggtag gccgtgttgt
     8701 gcagagccat gtcgaacagg ctcacgtcga tgtcccgtcc tgtgccgctc gtgcgcgccg
     8761 aggtgagcgc cgcgagcagc gcgtatgcaa gccccagccc cgtcatcagg tccacgatcg
     8821 aaagcccgaa gcgcgcgggc ggcgcgccgg gctcgccggt cagcgagaag tagccggcct
     8881 ccgcctgcat caggtagtcg aaccccggcc agtcggcgcg cgggccgctg cgcccgtagg
     8941 cggagagatg cgcgcatacg attttcgggt tatgcgcctt gagccggtcg taggtaagtc
     9001 cgagtttcgc cggcacgtcg ccacggagat tcgaggcgag cgcgtccgcc gtatgcacga
     9061 gatcgtgcag caccgcctgc ccttccgggc gtcccaggtc gagcgtcaag ctcttcttgt
     9121 tgcggttgaa gctgtggaag aactcgctgt cgccgggcgc gaagaagtgc ggcccgaccg
     9181 cgcgcgacat gtcgccgccg tcgttggggt tctcgatctt gatcacttcc gcgccctggt
     9241 cggcgaggaa catcgtaccg aaggggccgg ccccgtactg ctcgaccgcc agcacgcgca
     9301 caccgctcag gggcagcatg gcaaacccct aaccacggag agcactgaga aaatcaaatg
     9361 cacgaatcga accgcagaga atgggagaga accaaaatca taattgaacc gccaagacgc
     9421 caagagaatc gagaataaag acaggaagtt tctagaggtc tggtctttac tttgcaatca
     9481 agacctgaaa gcaaagaccc gaccccttag caacggagga atgagatcgg gtcgaatcac
     9541 gacccacggc cttatcattc gatcctgatg tccgcctgcc tgatcacttt ggcgtatttc
     9601 tcgatttcgc gcttgagcag cgccgacagc tcctcgggac tgctggcgac gggatccagg
     9661 gcctccctcg gcatgaattc ccggatctcc ggcgagtcga gcgccttgcg gatctccgag
     9721 ttcatcctgt ccacgattgg cctcggcgtg cccttcggga agtacatcgc gtaccagttg
     9781 tccgactcga agcccggata gaccgagttc atcgtcggca aatccggcag cgccgaggat
     9841 cgcttcggcg tggtaaccgc gagcgccttc gccttgccgg tcctcaggtg cggcatgatc
     9901 gaaggcgccg tggcgaagag ataatcgact tctccggaga tcaacgccag taccgccggc
     9961 ccgccaccgc ggtaggtgag cacggtggac ttgatgccgg cgacctggtt gagcatttcg
    10021 ccggtgagat ggctggtgct gccggcggta ttgccggccg cgttcatgtt cccggacgtt
    10081 cgcttcgcga gcgccacgag ctccttcacc gacttcactg gaacgctcgg gtgcacggac
    10141 aataccagcg gcgtcgagga aatccagctg atcggcgcga ggtcggtgag cgcgttgaac
    10201 ttcatgcgct tggcgtagag ggtgacggcc accgagaggc tggcggaggt gaagaggatg
    10261 gtgtagccgt ccggcggcga atccactgcg agctggcccg cgatcaagcc gccggcgccg
    10321 gtccggttat cgatgatgaa gttctgcccc gtgctcttct gaaacgcgga cgacagcagc
    10381 cgcgcctgga tatccgtccc tccccccggc gggaagggca ccaggatgcg cacgggtttg
    10441 tcgggccact gctgggctgc ggcctgcatt ggagtaacga cacaaatcgc ggccaccgcc
    10501 gccagtgcac atgcttgttt tctcatctga acctcctcct gatagaagac agctttgtga
    10561 ttgtgccgtc atgctagcac ttcgccgtgt acctgatacc ggtgatgagt gacgggtgat
    10621 gagtattttg cttcgatgct gtaaatcctg ttgatcctgt ctatttttgt ttgattttat
    10681 ctgcgtttat ctgcggttcc gttcttgtat ttcttggcgt ccttggcggt ttaattttgg
    10741 tttctcttgg cggcttggcg gttcaattat tattcttgaa tctctctgtg ttctctgtgt
    10801 ctccgtggtt aatgcatttc aggcggggtt ccgcttcacc agctcgcgcg cgatgatgat
    10861 gcgctgaagc tcgttggtcc cctcgccgat cgcgagcagc ggcgcgtcgc ggtagtagcg
    10921 ctcgatgttg aactccttcg agtagccgta ggcgccgtga atgcgcatcg cctccaggct
    10981 gttctcgagc gccgcctcgg tcgcgtagag cttcgccatc ccggcctcga gatcgcagcg
    11041 cccgccagcg tcgtaggcgc gcgcggccgc ctcggtgagc aggcgcgcgg cctcgacccg
    11101 cgtcgccatg tcggcgagct tgatctggat cgcctggtgc tccgcgatcg gcttgccgaa
    11161 ggtcttgcgc tgctgcgcgt agcgcaacga ctcctccagg cacgcgcgcg cgagccccgc
    11221 cccgcgcgcc gcgacgttga tgcgcccgag ctcgagcccg ttcagtactt gctgcagtcc
    11281 gcggccttcc tcggtcccga cccggttcgc ggccggcacc cggcaatcct cgaacagcag
    11341 ctcggctgtg tcgatgccgc ggtagccgag cttctcgagc ttgcgcacga ccttgaagcc
    11401 cggccccttc tcgatcagca gcaggctcat gcccttgtgc ggcgggcttg cccacggatc
    11461 ggtcttcacc agtaccgcca atatctgacc ctcggcgctg ttggtgatcc aggtcttggc
    11521 gccgttcacc acgtagtgat caccctcgcg gcgcgcgtgg gtgcggatcg cctggagatc
    11581 ggtgccgcag tcgggctcgg tcagcgcgag gccgccgcga agttctccgc tcgaaaagcg
    11641 cggcaggaag cggcgtttct gctccgccgt gccgtaacgc tccaccgccg ccgccatgat
    11701 gagatgcgaa ttgaagaatc ccgggaccga catccatacc gccgccatgc gctcgacgat
    11761 ccgcgcatag gtcaccgccg tgagcccgag cccgccgtgc tcgggcgcga tggtggcgcc
    11821 gaacagcccg agcgtcttca gcttctcgac gattgcggcc gggtaggtat cggcggcctc
    11881 cagcgcgtgc acgaccgggc gcacgtcccg ctcgagaaag cggtccactg catcaagaat
    11941 caggcgctcc tggtcctcat ttgcgagatt ttccggtgcc gcgaccatgt taacgagcaa
    12001 ggatgaaggc ggaaggatga aagatgagct atctccggca gctgcgctaa atctttgtct
    12061 tgtatggttc actgttggcg cttcgcgcgc tcgcatcgaa ggctctggat catcagcggt
    12121 agttcattcc ggttctcttg gcgtcttggc ggttcaattg ttgttcttgt ttctctccct
    12181 gtctctgcgg ttcattagta gcctacgtcg tcgtccaccg cgtgcccgcg cttgggcacg
    12241 aggatcgtgc gctcgaagct catgaacgcc gtgccgtcgg ccttcttgcc ggtggtgcgc
    12301 acagtgacga ttccctgcgt tggccgtgac ttcgactcgc gcacctccag cacctcggac
    12361 tcggcgtaga tcgtgtcgcc ggcgaacacc ggcgcggtga gccgcacctt gtcccagccg
    12421 aggttggcga ctgccttctg gcttacgtcg ctcacgctca tgccggtgac catcgcaagc
    12481 gtcaggcagc tgtccaccag cggccggccg aattcggtct tggcgctgta ggcggcgtcg
    12541 aagtgcagcg ggttggtatt catggtcaac agcgtgaacc aggtgttgtc ggcctccgag
    12601 atcgtgcgac ccggccggtg ctcatagacg tcgccgacct tgaaatcctc gaggtaacgg
    12661 ccgaaggact cgcggtagcg gttcggcccc actttcttgc gctcaactgt cattgttttc
    12721 actaacggga gggcgggaga gcgggagaga gtaagggcgg gaaaacaggt tctctccacc
    12781 tcccgatctc ccgatttcac gatctcccgg cggcagcgcg tcgcccgctt gcctgctcgg
    12841 gccgcgtcag ccgaagtatg gcgccaacgt ccttcgcctg ctcgagcgca gccagcgcgt
    12901 ccgcgagctc gtcgcgccgg cgcccggaga ggtacgggcc ggcaagcccg tcgaacttgg
    12961 cgcgcagctc ggccgcggag agaaagttcg ccggctcgcc cttcggcacc cgcacgtagg
    13021 tctcgaacgc gccgcgcgcg gtcttgatcc gcacgctccc gctcatttcc gccgggaagt
    13081 cggcctgggc tttcggatcc actcgggtgt gcacccgctt gcacaacgcc agcgtggcgg
    13141 gatcgccgag gtggcgcgcg tagtcgtccc ac
//
LOCUS       DBNGWZ010000104         9699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3158635, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000104 DBNGWZ010000000
VERSION     DBNGWZ010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3158635"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..170
                     /locus_tag="AC16CX_03075"
     CDS             9..170
                     /locus_tag="AC16CX_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011525.1"
                     /translation="MASIVADECFWDLDGPIVRITTPHVPLPAADQLEDNAIPSVDRI
                     VREIREKVD"
     gene            193..1890
                     /locus_tag="AC16CX_03080"
     CDS             193..1890
                     /locus_tag="AC16CX_03080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR3011526.1"
                     /translation="MKFESVFRHVALVFGVVLSLAGAGCAQIGARPEVVRHADMVLVN
                     GKIVTVDKNFSLQEAVAIRDGKILAVGTTSNIRSLAGDQTKVVDLKGRTVIPGLIDSH
                     IHFFRASFTWAREVRLDQARSVQDILGLVEKRASRLKPGDWILTYGGWHYSQLKEGRA
                     PTRQELDRVAPNNPVLLRILLPHPSTHFAVLNSAALKAAEITRDTKDPPNGVIEKDSA
                     GEPTGVLRRAAVPLALRKVPRASLEEKVEGLKLVMRDFHAAGLTSVIETVGGGVTDDD
                     YKVLFESWRRNELTMRTALLIYTAKFEDGQRWIKHLPIRFGDDWLRVSGFGEQLVSAV
                     QDNLAPSFPLKPEGLEEYRKFVTEAAKNRWPIQQHTTLGTTAKAYLEIYEEVDRQYPI
                     RDLRWSLAHVETISEADIQRAKRLGVGLTIQSRQVIQSELMKQAWGDVAHSVPPLKKM
                     LAIGVPVGAGTDATVVTPYHPFTTLWWLVTGKDWAGRVVRPNDRLSREDALRIHTVGS
                     AWFSFDEKIKGSIEPGKLADLVALTDDYLTVPEDKIREISSVMTIVGGKIVYEAQKQ"
     gene            2075..3334
                     /locus_tag="AC16CX_03085"
     CDS             2075..3334
                     /locus_tag="AC16CX_03085"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3011527.1"
                     /translation="MPQLGETVAEGTVTKWYKKVGDAVRADETLFDVETDKVSTEIPA
                     PTSGVLAEILVAEGATAKVGTRLAVIHESAPHQAPGSSAASTAAPIREVPEPTPTVTG
                     PRTTSQGAVRGAPSERAGIAAAAARLSPVVSRLIAEHGLNPSDIRGTGRDGRITREDV
                     LTHISQREGKPSAPARPAALAGAEETAPLNIIRKRTAEHMAKAWTTVPHVLQAVEADF
                     SRVEQARRMSGEQWKKREGFSLTYLPFIAHAVAAALIKYPRLNASFRGDHLVLHRRVN
                     LGIAVDLNFEGLLAPVAKDAAAMTLLQLARAINDLAARARAGKLRPDDMTECTYTITN
                     NGAFGTLITAPIISPPQVAILSTDAVRKRAVVIESEQGDTIAVRPMGMLAQAFDHRAV
                     DGAYAAAFLNEVKTLIETTEWLQKLQP"
     gene            3432..4289
                     /locus_tag="AC16CX_03090"
     CDS             3432..4289
                     /locus_tag="AC16CX_03090"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3011528.1"
                     /translation="MTAEAKKSRALVDPAWVHRHRNDPGVRLIEIAGMNQDEMQAYRG
                     GHVPGAVCWKWKEMLWDPYARDFPAPEEFARRCGEAGIGNDTTVVFYGEGVQFGFYAW
                     WAFRYCGHARVKVLDGARYRWAAEGRPLVTDEPLPAAPVSYRPVKRVERMRILRDKVL
                     AALRRNGTVILDGRSPEEYSGERVGGPGGPDVGALRYGRIPGARHLYFEELLDANKAF
                     KPTTELQQLVAARGAAPDRDIVTYCRMSHRATVLYFALTQLLGYRKVRVYDGSWTEWG
                     NLVGVPVER"
     gene            4393..4845
                     /locus_tag="AC16CX_03095"
     CDS             4393..4845
                     /locus_tag="AC16CX_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011529.1"
                     /translation="MCSRQARVLVWMALGGVIAASAAVAQAKECYSPDARSKERAFSR
                     AVITEGGKTIWLAGQTAAANLDFEGQTREIFANLDKTIKAVGGSGLRDMVTMTVFIND
                     VRYGDRFVEIRKEIFKECFPGSALITVTGFARPGVMVEVQGIAVVGGK"
     gene            5030..5968
                     /locus_tag="AC16CX_03100"
     CDS             5030..5968
                     /locus_tag="AC16CX_03100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011530.1"
                     /translation="MATANMKAHRLGWIGIGRMGYAMAERLAKAGCDITVWNRTREKA
                     EPLAKSGAKIAGSLPELAACDIVFTMVSAGKDVKEVLFGANGVTSKGKAPKIVVDSTS
                     ISLEESAEIRGKLAKQGVKLLAAPVSGNAKVIKAGKLTVVASGPKDAFDAVSPYLAAI
                     GQGVSYVGDGELSRIAKICHNVMLGVVIQNLCEITVLAEKAGMKRHAFLDFLNKSVMG
                     STFTRYKTPALVNLDFHVTFTPELLRKDIDLGLSAGREFGVPMPLTSLARDMVQTLIG
                     HGYTEQDFSALLLLQAKASGIELKPEHVEVSDGLGS"
     gene            5986..6381
                     /locus_tag="AC16CX_03105"
     CDS             5986..6381
                     /locus_tag="AC16CX_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011531.1"
                     /translation="MAAKVEYLEGTDFQKSRAFSPAVITQGGKIVWLAGQTTTVDESG
                     KDISGNCEAQARTIFALMDRTLKRAGGSLANLVTMTVFINDPRYGDSFVKLRGEIFGD
                     GRYPASALITVSGFSRPGVVIEIQGVAVI"
     gene            6439..7401
                     /locus_tag="AC16CX_03110"
     CDS             6439..7401
                     /locus_tag="AC16CX_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011532.1"
                     /translation="MKTVMKVAAFALALAALQAFAQNYPARPVRAIIAFPPGSATDII
                     GRVITAKVSELWGQQVVADNRGGAGGSIASALAAKSAPDGYTLIINSGAHTVMPSMYA
                     KLPYDTVKDFVDIMPLVAMPNVLVVNPSSRLKSLGDFIADAKASPGKINFAFAGLGSG
                     THLNSEKFKLEAKIDFTLVAYKGSGEIFPDLLAGRVDTYFAPLSAGLPYIQSGKLRPL
                     AVTSTKRVSQLPDVPTMAESGVPKFEFLLWFGLWGPSGIPAPVLARIRKGFGDALADP
                     GVREKLATLGNTVMTMTPAEFGKFVRQEIAVNARIFKAAGIKPQ"
     gene            7443..8564
                     /locus_tag="AC16CX_03115"
     CDS             7443..8564
                     /locus_tag="AC16CX_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3011533.1"
                     /translation="MGDQYPTVKVAAVQAASVFLDRGGSTEKACRLIREAGRNGARVI
                     GFPEGFIPAHPVWYHHHPATSAIANRLAVELFKNSVEIPGPEIEALCAAACEANAYVV
                     MGVCEKIPNTIGTMFNTQVFIGSGGRLLGKHQKLMPTVGERLVHMGGFGDTLAAIQTE
                     FGPMTGLICGENSNPLAIFAMTAEGTRIHVMSWPNHFPMGGDPMRNRVSVDSQAFAQM
                     SKAWVVSACGTVDEDMIRRLEVGPEGEKFLRSADCCGGSVIVSPGSRIVAGPMGAEEG
                     ILYAECNLELGVLTKLRHDFAGHYNRPDVFQLQVNRAAPRIYTVHTTPEQLAPARPRS
                     NTGSGTTPRELPGSAAQRTLPGPRAGRRARVPRKRGNRR"
     gene            8682..>9699
                     /locus_tag="AC16CX_03120"
     CDS             8682..>9699
                     /locus_tag="AC16CX_03120"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3011534.1"
                     /translation="MITELDCRGRSTPEAAATLIAHYNACRPGDRFQAWLDDYSCGLR
                     VWLLEAGARHGSEQLAEGWRLTIERTLSPAQGSIPGVHHVIARGDSVWTCERAHRVAR
                     LDAQDGRVAASRAAAQKASHLALSADGKRLFIADSEANAMIALDARDLSEIARWDAPG
                     GPQLPLASADGVVCVTGSASGTLTIARPTTDGYAAQTIEVGSCPHDPVLTADGACVFV
                     PCTGDAQLVKVRLKGGAIVGRCAVDNGPAHLALHPDGSRIYSANSWDGTVTCITVEGE
                     RIGHAASGGWAHAIDITPNGRTVWVANFLDDTLAVFDAGTLERLTVLPTEPYAHGLDV
                     SADGR"
BASE COUNT         1841 a         2981 c         3303 g         1574 t
ORIGIN      
        1 gcgctgaaat tgcttccatc gtcgccgacg agtgcttctg ggacctcgac gggccgatcg
       61 tgcgcatcac cacgccgcat gtgccgctgc ccgcggccga ccagctggag gacaacgcga
      121 tcccgtcagt tgatcgcatc gtgcgcgaga tccgggagaa ggtcgattaa caagcaagga
      181 aagggggaga tcatgaagtt cgagagtgtg ttcagacatg ttgccctggt attcggggtg
      241 gtgttgtcgc tcgccggcgc ggggtgcgcc cagatcggtg cccgacccga ggtggtccgg
      301 catgccgaca tggtcctagt caacggcaag atcgtgaccg tggacaagaa cttctccctg
      361 caggaagccg tcgcgatccg ggacggcaag atcctggcgg tgggtaccac gtcgaacatc
      421 cggtcgctgg cgggcgatca gacaaaggtc gtcgatctga aggggcgcac ggtgattccc
      481 ggcctcatcg attcccacat ccattttttc cgcgcaagct ttacctgggc gcgtgaggtc
      541 cgcctggacc aggcgcgctc cgtccaggat atcctcggcc tcgtcgagaa gcgggcgagc
      601 cggctcaagc cgggagactg gattctcacc tacgggggct ggcactacag ccagctgaag
      661 gaagggcgtg cgcccacgcg ccaggaactg gaccgcgtgg cgcccaacaa cccggtgctg
      721 ctgcggattc tccttccaca tccttcgact cacttcgcgg tgcttaacag cgctgctctg
      781 aaggccgctg agattacgcg cgataccaag gacccgccca acggcgttat cgaaaaagac
      841 tcggcgggag agccgacggg agtcctgcgg cgggcggcgg tcccgctcgc cttacgcaag
      901 gtaccccggg cgagcctcga ggagaaggtc gagggcctga aactcgtcat gcgggacttc
      961 cacgccgcgg gcctcacgag cgtgatcgag acggtcggag gcggcgtgac ggatgacgac
     1021 tacaaagtgc tgttcgagtc gtggcggagg aatgagctga cgatgaggac ggcgcttctc
     1081 atctacaccg cgaaattcga ggacggccag cgctggatca agcacctgcc gatcaggttc
     1141 ggggacgact ggctgagggt aagcggcttt ggcgagcagc tggtgagtgc ggtgcaggat
     1201 aaccttgccc cgagctttcc cctcaagccc gaaggcctgg aggagtaccg gaagttcgtg
     1261 accgaagcgg cgaagaaccg gtggccgatc cagcagcaca cgacgctcgg gacgacagcc
     1321 aaagcctacc tcgaaattta cgaggaggtg gatcggcagt acccgatccg cgacctcagg
     1381 tggtcgctcg cccacgtgga aacgatctcg gaggcggaca tccagcgggc aaagcgcctc
     1441 ggcgtcggac tcacgatcca gagccggcag gtgattcaaa gcgaactgat gaaacaggcc
     1501 tggggggatg tggctcactc tgtcccgcct ctgaagaaaa tgttggcgat cggggtgccg
     1561 gtcggggcgg gaacggacgc cacggttgta accccttatc acccgttcac cacgctctgg
     1621 tggttggtga ccggcaaaga ttgggcgggc cgcgtcgttc gcccgaatga tcggctgagc
     1681 cgcgaggacg ccctgcgcat ccacaccgtg ggaagcgcct ggttttcgtt cgacgagaag
     1741 atcaagggct cgatcgagcc cgggaagctt gccgacctcg tcgcgctgac cgacgactac
     1801 ctcaccgtgc ccgaggacaa gattcgcgag atcagctcgg tcatgacgat cgtgggcggg
     1861 aagatcgttt acgaagccca aaagcagtga acggtgaaca gtgaacagtg aacagtgaac
     1921 agtgaacagt gaatggaatc cctctccccc gaacacccgg gggagaagtt gggtgagggg
     1981 gaatcaccat tcactattca ccattcactt tggatgcggg ttagtcaaag tcgtctgaga
     2041 gtgagttgag gcaaccttac cgatggatgt agtgatgccg cagctcggcg agacggtcgc
     2101 cgaaggcacg gtcaccaagt ggtacaagaa ggtcggcgac gcggtcaggg ccgacgagac
     2161 gctgttcgat gtcgaaaccg acaaggtaag caccgagatc ccggcgccga cctccggcgt
     2221 gctcgcggaa atcctggtcg cggaaggcgc gacggcgaag gttggaacgc ggctggcggt
     2281 catccacgaa tcggcgccgc accaggcacc cggcagttcc gcggcttcga cagcggcgcc
     2341 gataagggaa gtgcccgagc cgaccccaac ggtgactggg ccccggacga cgtcgcaagg
     2401 ggcggtgcga ggtgcgccgt ctgaacgagc gggaattgct gcagccgcgg cgcgattgtc
     2461 ccccgtcgtg agccgactca tcgctgaaca cggcctgaat ccgtctgata tacgcggcac
     2521 cgggcgcgac ggccgcatca cgcgcgagga tgtcctgacc catatctcgc aacgcgaggg
     2581 gaaaccttcc gcgcccgcgc gacccgcggc actggcaggc gctgaggaga cggcaccgct
     2641 caacatcatt cgcaagcgca cggccgagca catggcaaag gcctggacga cggtgccgca
     2701 cgtgctgcag gcggtggaag cggatttttc ccgcgtggaa caggcccgcc gcatgagcgg
     2761 ggagcaatgg aagaaacgcg aaggattttc cctgacctac ctgccgttca tcgcgcatgc
     2821 cgtggcggcg gccctgatca agtatccccg tttgaacgcc agcttccgcg gcgatcacct
     2881 cgtgctgcac cggcgcgtca acctcggcat cgcggtggac ctcaacttcg aggggctgct
     2941 cgcgccggtc gcgaaggacg ccgcagcaat gacgttgctg cagctcgcgc gcgccatcaa
     3001 cgatctcgcg gcccgggcgc gtgccggaaa gctgagaccc gacgacatga cggagtgcac
     3061 ctataccatc accaacaacg gcgcattcgg cacgctgatc accgcgccga tcatcagccc
     3121 gccgcaggta gcgatactct cgaccgacgc ggtacgcaag cgcgccgtgg tgatcgagag
     3181 cgaacaggga gacaccattg ccgtgcggcc gatggggatg ctcgcacagg ccttcgatca
     3241 tcgcgcggtg gacggcgcat atgcggcggc gttcctgaac gaggtgaaga cgctgatcga
     3301 gacaaccgaa tggctgcaga aactgcagcc atgaagtcga aagtcggaag tcgaatgtcg
     3361 aaagtgtttg tgccgttttt acttccgact ttcgcctttc gcctttcgac ttgtccgttc
     3421 agggcggaag gatgacggcc gaggcaaaga agtcgcgggc gctggtggat cccgcctggg
     3481 tgcaccggca ccgcaacgat cccggcgtca ggctgatcga gatcgcgggc atgaaccagg
     3541 acgagatgca ggcctaccgg ggcgggcacg tgccgggcgc ggtgtgctgg aagtggaagg
     3601 agatgctgtg ggatccttac gcccgcgatt ttcccgcgcc tgaggaattc gcgcgccgtt
     3661 gcggcgaggc gggcatcggc aatgacacca cagtggtgtt ctatggagag ggcgtgcagt
     3721 tcggcttcta tgcgtggtgg gcattccgct attgcgggca cgcccgggtc aaggtgctcg
     3781 acggggcgcg ctaccgctgg gccgcggagg gccggccgct ggtcaccgac gagccgctac
     3841 cggcggcgcc cgtgagctac cggccggtca aacgcgtcga gcggatgcgc atactccgcg
     3901 acaaggtgct cgcggcgctg cgccgaaacg ggacggtcat actcgacggg cgctcgcccg
     3961 aggaatacag cggagagcgc gtcggcggcc ccggcgggcc ggacgtgggc gcgctgcgtt
     4021 acggccggat tccgggcgca aggcacctct atttcgagga actgctcgac gccaacaagg
     4081 cattcaagcc gacgaccgaa ttgcagcagc tcgtggcggc acgcggcgcg gcacccgacc
     4141 gggacatcgt cacgtattgc cgcatgagcc accgcgcgac ggtgctgtat ttcgcactga
     4201 cccaactcct cggctaccgg aaggtgcgcg tctacgacgg ctcgtggacc gagtggggca
     4261 acctcgtcgg cgtgccggtc gagagatgaa gcagggatga gggcggaggg ctgagggatg
     4321 ggggaaaaac gaagaatcga gcgctttcag tcaaccttga tccgttaacg gtcgcgacta
     4381 atggaggtaa ccatgtgctc aagacaagct cgagtgttgg tatggatggc attggggggc
     4441 gtgatagcag catccgccgc cgttgcgcag gccaaggaat gctattcgcc tgatgcgcgg
     4501 tccaaggagc gcgcgttctc gcgcgccgtc atcaccgagg gtggcaagac catctggctc
     4561 gcgggccaga ctgccgcggc caacctcgat ttcgaaggcc agacgcgcga gatcttcgcg
     4621 aacctggaca agaccatcaa ggccgtgggc ggcagcggct tgagggacat ggtgacgatg
     4681 acggtattca tcaacgacgt gcggtatggc gaccgcttcg tggagatccg caaggagatc
     4741 ttcaaggaat gcttcccggg gagcgcgctc atcaccgtca ccggctttgc caggcccggc
     4801 gtcatggtcg aagtgcaggg catcgcggtc gtcggcggca aatagaagcc gggagagcgc
     4861 agaaggcaga aggcgaaagg cggaaaagca aggcgaaagt cgaaagtcga aagtagttgt
     4921 cggccgaagg ccaaaccgac ctggtctgtg cggacacttc cgcctttcga ctttcgcctt
     4981 tcgacttatt gcgatgtaat cggctgaatc gacaacggag aaacctggaa tggcaacggc
     5041 aaacatgaag gcgcatcgtc tcggctggat cggcatcggc cgcatgggtt acgcgatggc
     5101 cgagcggctg gcgaaagcgg gatgcgacat cacggtgtgg aaccgcaccc gcgagaaggc
     5161 cgagccgctc gcgaagtccg gcgcgaagat tgccggttcg cttcccgaac tcgccgcgtg
     5221 cgacatcgtc ttcaccatgg tgtcggccgg caaggacgtg aaggaagtgc tgttcggggc
     5281 gaacggcgtc acctcgaagg gcaaggcgcc gaagatcgtg gtggacagca cctcgatctc
     5341 gctcgaggaa tcggcggaaa tccgggggaa gctcgccaag cagggcgtga agctactggc
     5401 agcgccggtc tcgggcaatg ccaaagtgat caaggccggc aagctcacgg tggtcgcctc
     5461 cggaccgaag gacgcgttcg acgccgtatc gccctacctc gccgcgatcg ggcagggcgt
     5521 ctcgtacgtc ggggacggcg agctttcgcg catcgccaag atctgccaca acgtgatgct
     5581 cggggtggtg atccagaatc tgtgcgagat cacggtgctg gccgagaaag cgggcatgaa
     5641 acggcatgcc tttctcgact tcctcaacaa gagcgtcatg ggttcgacgt tcacccgcta
     5701 caagacgccg gcgctggtga atctcgattt tcacgtcacg ttcacgcccg agctgctgcg
     5761 caaggacatc gacctcggtc tctcggcggg cagggaattc ggcgtgccga tgccgctcac
     5821 gtcgcttgcc cgcgacatgg tgcagacgct catcggccac ggctatactg aacaggattt
     5881 ttccgcgctg ctcctgctgc aggcgaaggc ctccggcatc gaattgaagc ccgagcacgt
     5941 ggaagtgagc gacggtctgg ggtcgtgacc gcacgggaag gcacgatggc ggcgaaggtc
     6001 gaatacctcg agggcacgga tttccagaag agccgggcgt tttcgccggc cgtgattacc
     6061 cagggcggca agatcgtctg gctggccggg cagaccacga ccgtcgacga gtccgggaag
     6121 gacatctccg gcaattgcga agcgcaggcg cggaccatct tcgcgctgat ggaccggacg
     6181 ctgaagcgcg ccgggggctc gctcgccaac ctggtcacga tgacggtgtt catcaatgat
     6241 ccgcgctacg gcgactcgtt cgtgaagctg cgcggcgaga tcttcgggga cgggcgctat
     6301 cccgcgagcg cgctgatcac cgtcagcggt ttctcacgcc cgggggtcgt gatcgagatc
     6361 cagggtgtcg cggtgattta gcgcgctggg cgaaatgcaa cttaccctat tggtctgaat
     6421 tgtcggggag gaaatgccat gaaaacggtc atgaaagtcg ctgcattcgc cttggcgctc
     6481 gcggcgctgc aggctttcgc ccagaactat cccgcccggc cggtgcgcgc gatcatcgcg
     6541 tttccgccgg gcagcgcgac cgacatcatc ggccgcgtca tcacggcgaa ggtttcggaa
     6601 ttgtggggcc agcaggtcgt ggcggacaac cgcggcggcg cgggcgggtc gatcgcctcg
     6661 gcgctcgcgg cgaagtcggc acccgacggc tacacgctga tcatcaactc cggcgcgcat
     6721 acggtcatgc cgtccatgta cgccaagctg ccctacgaca cggtgaagga cttcgtcgac
     6781 atcatgccgc tggtggccat gcccaacgtg ctggtcgtca acccgagctc gaggctcaag
     6841 tcgctcggcg atttcatcgc cgacgccaag gcgagcccgg gaaagatcaa tttcgctttc
     6901 gccgggctgg gcagcggcac gcacctcaat tccgagaaat tcaagctcga agccaagatc
     6961 gacttcacgc tggtcgcgta caagggctcg ggggagatct tccctgacct cctggccggg
     7021 cgcgtcgata cctacttcgc gccgctgtcg gcggggctgc cgtacatcca gtcgggcaag
     7081 ctgcggccgc tggcggtgac gtcgacgaag cgcgtgagcc agcttccgga cgtgcccacc
     7141 atggccgagt cgggcgtgcc gaagttcgaa ttcctgctgt ggttcgggct gtgggggcct
     7201 tcgggcattc ccgcgccggt gctggccagg atccggaagg gcttcgggga cgcgctcgcc
     7261 gaccccggcg tgcgcgagaa gctcgctacg cttggcaaca ccgtcatgac catgactccg
     7321 gcggaattcg gcaagttcgt gcgccaggag atcgccgtca acgcgcgcat attcaaggcg
     7381 gccggcatca agccgcaata acgccaggag cgtgaatcgt gattcatgag tcgtgattcg
     7441 agatgggcga ccagtacccg accgtgaaag tggccgccgt gcaggcggca tccgttttcc
     7501 tcgaccgcgg gggctcgacc gaaaaggcct gccgcctgat ccgcgaggcg ggacgcaacg
     7561 gggcgcgcgt catcggcttc ccggaggggt tcatcccggc gcaccccgtg tggtaccacc
     7621 atcacccggc gaccagcgcc atcgccaacc ggctggccgt cgagctgttc aagaactcgg
     7681 tggagatccc cggccccgaa atcgaggcgc tgtgcgcggc ggcgtgcgag gccaacgcct
     7741 acgtggtgat gggtgtgtgc gagaagattc ccaacaccat cggcaccatg ttcaacaccc
     7801 aggtcttcat cgggtccggc ggcaggctcc tcgggaagca ccagaagctg atgcccaccg
     7861 tgggcgagcg gctggtgcac atgggcggct tcggcgatac gctcgccgcg atccagaccg
     7921 agttcgggcc gatgaccggt ctcatctgcg gcgagaactc caacccgctc gcgatcttcg
     7981 ccatgaccgc cgagggcacg cgcatccacg tcatgagctg gcccaatcac ttcccgatgg
     8041 gcggcgaccc gatgcgcaac cgcgtatcgg tggattcgca ggcgttcgcc cagatgagca
     8101 aggcgtgggt ggtgtcggcg tgcggcaccg tggacgagga catgatccgg agactggagg
     8161 tcgggccgga gggcgagaaa ttcctgcgca gcgccgactg ctgcggcggc agcgtcatcg
     8221 tctcgcccgg cagccgcatc gtggccggcc cgatgggcgc cgaggagggc atcctctacg
     8281 cggaatgcaa cctcgagctg ggcgtgctca ccaagctgcg ccacgacttc gccgggcact
     8341 acaaccggcc cgacgtcttc cagcttcagg tgaaccgtgc tgctccccga atttacacgg
     8401 tgcatacgac tccggaacaa cttgcgcccg cccggccaag gagcaatacg gggagcggca
     8461 ccacgccgcg cgaattaccc gggtcagccg ctcaacggac actgcccggg ccgagggcag
     8521 ggcgccgggc gcgcgtgccg cgcaagcgcg ggaacagacg ttagaaaacg ctgccaatct
     8581 cagggctgca ctgccaggga tgccggggga aggcaaatta tggtaagaaa taaatcaagc
     8641 cggcttccgg gtccattttc ttacgattgc ggttcgccgg cttgattacc gaactcgact
     8701 gtcgtggccg gtccaccccg gaagcagcgg ccacgctgat cgcgcattac aacgcgtgca
     8761 ggccgggtga ccggttccag gcttggcttg acgattattc ctgcggtctg cgcgtgtggc
     8821 tgttggaggc tggcgcgcgg cacggcagcg aacagctggc ggaaggctgg cggctcacta
     8881 ttgagcggac gctctcgccg gcgcagggct cgattccagg cgtgcaccac gtcatcgcgc
     8941 gcggcgattc ggtgtggacg tgcgagcgcg cacaccgggt cgcccgcctg gacgcgcagg
     9001 acggccgcgt ggctgcgagc cgggcggcgg cgcaaaaagc ttcgcatctc gccttgagcg
     9061 cagacgggaa gcggctgttc atcgccgaca gcgaagcaaa cgccatgatc gcgctcgacg
     9121 cgcgggacct ttccgaaatc gcgcggtggg acgcgccggg cggtccgcag cttccgctcg
     9181 cttctgccga cggagtggtg tgcgtgacgg gaagcgcgag tggcacgttg accatcgcgc
     9241 ggccaacgac cgatggctac gctgcacaga cgatcgaggt gggaagctgc ccgcacgacc
     9301 ccgtattaac ggccgacggc gcctgcgtgt ttgtcccgtg cactggcgat gcgcaactcg
     9361 tcaaagtccg gctgaagggc ggcgcgattg tcggccgttg cgcggtggac aacggccctg
     9421 cacacctcgc gcttcacccg gacggcagcc gcatctattc ggcgaacagc tgggacggca
     9481 ccgtcacctg cataacggtg gaaggcgagc gcatcggtca cgccgcgagc ggcggctggg
     9541 cgcacgcgat cgacatcacg cccaacggcc gcacggtgtg ggtggcgaac ttcctcgacg
     9601 acacgctggc ggtgttcgac gccggcacgc ttgagcgcct gaccgtactg ccgacggaac
     9661 cctacgcgca tggcctcgac gtgtcggccg acggccgct
//
LOCUS       DBNGWZ010000105        13302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2025088, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000105 DBNGWZ010000000
VERSION     DBNGWZ010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2025088"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="AC16CX_03125"
     CDS             complement(<1..1316)
                     /locus_tag="AC16CX_03125"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011535.1"
                     /translation="MEVTRTLAKFIVRHRYDDIPEKVRHETARSFLNWVGCAVGASRH
                     ETVERALAALSEFSGPREATVLGRGDRLDIMLAALMNGTTSHTFDFDDTHLKTIIHPS
                     GPVASAILALAEHKPVNGEEFLHAFILGVETECRIGNAVYPSHYDVGWHITATAGVFG
                     AAAAAGRLLGLTEQQMVWALGIAATQSSGLREMFGSMVKPMHPGNAARNGLLAALLAS
                     RNFTSSEQGIEGRRAFANVLATERNLGEITGKLGETWEISLNTYKPFACGIVEHPAID
                     GCIQLRNAHQLKAGDIESISLKAHPLVLELTGKKAPQSGLEGKFSVYHSCAVAVIHGA
                     AGEAQYSDEAVRDPGVIALRDRVTATVDRAMHEDQVHVSIKLKNGKTLEKYIEHAVGS
                     LDHPMSDADLEAKFRGLAEGILSRAETDKLIRLCWDVGKLKDVGEVA"
     gene            complement(1380..2336)
                     /locus_tag="AC16CX_03130"
     CDS             complement(1380..2336)
                     /locus_tag="AC16CX_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011536.1"
                     /translation="MLRIAAVFLLLAFSFLAKAQTYPAKPVRIVVPFAPGGGADIIAR
                     ILGQKMTESWGQQVVVDNRAGASGNIGAEIVAKSAPDGYTLLMASSALAINPSVYKSV
                     PYDAVRDFAPITQPGLLPNILVVHPSVPVKTVKDLVALARSKPGQLSYASAGAGTGTH
                     LAAEMFKIIAGVDMVHVPYKGGGALIGDLLGGQVALTFATLPSVMPYVKAGRLRAVAM
                     TTAKRWRGLPELPTMAESGFKGFEISTWIGLLAPAGTPREIVTKQHGEVVRILKLADV
                     RERFEALGIEPVGDTPEQFAQYIKAELAKYAKIAKQSGARVD"
     gene            complement(2345..3226)
                     /locus_tag="AC16CX_03135"
     CDS             complement(2345..3226)
                     /locus_tag="AC16CX_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3011537.1"
                     /translation="MKEAPRRILRRRLTEGPVVIAPGIYDAYGARLVEQAGFEAVYMT
                     GNGVSASLLGRPDVGLVDLTLITGQAHRVASCVDIPLICDADTGYGNAVNVRRTVEEF
                     EAAGVAAIHIEDQVSPKRCGQLPGARPVIDLREAIGKVEAAVAARRDPDFIIIARTDS
                     AEAHGLDEAIKRGKAFRKAGADVIFVEMKGGPSILEDLKRVTSEVDAPCLVNMGAGGK
                     LDELDVEGIGRLGLRIAIYPGLERAAAGYAMREALAALKRDGNTKAVRDRMLTLKEYN
                     EALKLSEIEEWERKYLR"
     gene            complement(3223..4350)
                     /locus_tag="AC16CX_03140"
     CDS             complement(3223..4350)
                     /locus_tag="AC16CX_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3011538.1"
                     /translation="MKIERVEVFGVAMPLIGEYKNAYLTKSVQKSAIVRIIASGGVTG
                     LGNIDPTPGYSKERPEEHLQMLQTRLAPLLMGMDPTNVHAVLARVESAVKGFLDSKAA
                     IEMACVDIAARVANMPVHAYLGGAVKDRLLFNAWIGILPPDQAARETLEWKNRGFRSA
                     KIKVGGDIEADRDRVKAVREAVGQDFQLRIDANAGYDADTSIKLARMVAPFKLQLFEQ
                     PVPDHDIAGMARVRREANAVGVPVMADESVLDHASLIRIIKAEAADIVKVKVMKQGGF
                     LNTRRMIATAEAAGIRCVVGHGFGLGVNTMAEIMLAATSGNVIDGLECVGPLKTADDI
                     VTRKLDLSSGSLPLPGGPGLGVDLDDAKLAKYRFDIRQAAA"
     gene            complement(4463..4972)
                     /locus_tag="AC16CX_03145"
     CDS             complement(4463..4972)
                     /locus_tag="AC16CX_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3011539.1"
                     /translation="MTTFIRGKAHLLGDDINTDVHCSSKYHPGRDNAYVAQHAFEQLA
                     PGFAARFASGDLIVAGRNFGNNSSREQAVHIMRLMGVSAIVAASFGRQFFRNCINNGL
                     PVVECDMAGVAEGDEIEIDLAGGRVAVPGRGIVREVSPLPGEVQAILAAGGLIPFLKA
                     HPEWRLSSQ"
     gene            complement(5007..6299)
                     /locus_tag="AC16CX_03150"
     CDS             complement(5007..6299)
                     /locus_tag="AC16CX_03150"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HIR3011540.1"
                     /translation="MGATIAEKILGVHASGKAAKAGEVVVARVDYAMLHDARASNALK
                     RIEQFGSAKLPFAARTAFVLDHYSPPPHPEAANIHRAMRAFAEEHGVQLYDVGDGICH
                     QVLPEGGHLTCGDLVTGTDTHSTTYGAFNAFGTGVEGTDLTAVLMTGKLWFRVPETIR
                     VDLKGRLQPGVWAKDITLRMLGRLGAEGANYKAIEYAGMAVAAMEIDDRMTLANHAAE
                     LGAKAALLEADEKTLAWLKARGARDPQPVKADPDARYVERIEIDASRLAPQVARKHRI
                     DDVVGVTEIKDQRVHYALIGTCTNGRLDDLRQAAAILKGRRIAKGVRMIVTPASRNVY
                     LQAAREGLIEALTQAGASVEAAGCGTCVNITGHLITGDGDVVISSANRNFKGRLGNPD
                     SDIFIASAATVAASALTGCITDPRTVAGGEWRPISKAA"
     gene            6530..7954
                     /locus_tag="AC16CX_03155"
     CDS             6530..7954
                     /locus_tag="AC16CX_03155"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011541.1"
                     /translation="MPAAPEISLTSYVAEFITMTKASGIPADVMHLGKRSILDGIGLA
                     LAGNAAESGQIVRQYLKSLGCPTDKGCTVIGTNLKVPARFAAFANGIAIHADDYDDTQ
                     LAVARDRVYGLLTHPTAPALPPVLALGELANHPGSDVLAAYQVAVEVETKVAEAINPR
                     HYDDGFHSTATIGAIGAAAGAARLLGLDTEQTQRALGIGASQSAGLRENFGTMTKPFH
                     PGRSAESGVVAAEFAKLGWTATPIVLEAGRGFFQAAGGGYDTKAIEGKLGSPWTFAFP
                     GVSIKPHPSGSLTHPGMGLMLDLVRKHDIRPAQVVKVKVGTNRHMPNALIHHRPKNEL
                     QAKFSMEFCMAILLLERKAGLAEFTDEVVNRPDVRKMIEKVEFSVHPEAEAAGYEKMT
                     TIIDIELTGGRRLGGRADFGKGSPANPMSDDELADKFRECAAWGRLPKANAEKVIDLV
                     FDLEKLKSIRELTRLLAVGRKRVK"
     gene            8095..9117
                     /locus_tag="AC16CX_03160"
     CDS             8095..9117
                     /locus_tag="AC16CX_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PdxA family dehydrogenase"
                     /protein_id="HIR3011542.1"
                     /translation="MKKDLPVIGLMLGDMTGIGPEICAKLIASGTPGEVARVVVIGDA
                     RVLDLGMRDAGVKLGYRIHSGVDDVRWPVEALPVVDLGNIDPSRIKRGEVSAESGRLT
                     GETLKYMIDLALAGKLDGICFAPLNKMALHQGGWKFHDEHQMFAKLTGHSGFFGEMNV
                     IPEFSTFRVTSHIALREAVNLISPERIAAAVRLADTTLRAMGHRRPRIGVAALNPHGG
                     ENGLFGDEEIRIIRPAVDKLRESGIGCIGPIPSDTIFLRALKGQFDGVVMMYHDQGQI
                     ATKLIGFNKGVTVTAGLKTVFATPAHGTAFDIVGQGRADYNALEYAFRLAARLASNTG
                     RGVGAT"
     gene            9165..10145
                     /locus_tag="AC16CX_03165"
     CDS             9165..10145
                     /locus_tag="AC16CX_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011543.1"
                     /translation="MKISIHGMVFNSICGMLVAAALPSTAARAADWPTKPIRLVIGFA
                     PGGGTDTTSRALSTKLTTSFGQQVIVDNRPGHSGTIAADIVAKAVPDGHTLLLGTIAL
                     VINPSLTVKMPFDTLKDLAPVTRAVDSTNILVVHPSVAAKSVKELIALAKTKTLNGGS
                     SGIGGTGHLAVELFNLQAGTKITHVPYKGGGPAIVDLLAGNIQLIFATAASSIAHINA
                     GKIRPLAVTTVKRSALVPELPTVAEAGLPGFEANNWNGFLVPAKTPRPIINRLNKELT
                     AALLSPDIKEFLFKQGLDAAPDNPEAFGAYMKSEMAKWAKVIKAAGIKPN"
     gene            10169..11152
                     /locus_tag="AC16CX_03170"
     CDS             10169..11152
                     /locus_tag="AC16CX_03170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011544.1"
                     /translation="MSDEKPLRGLAHAAIVCVSLAIAAPIAAQEYPTKPVRIIIGFPP
                     GGATDLVSRLMAPKYAGIFKQQFIVDNRPGANGVIGSDLAAKAAPDGYAIHLATIGSL
                     VLSPATGKVPYDPIKDFAPVSQAVALQNIFIVHPTLPARTMKELIALAKARPGKLNFA
                     SSGNASPGHLAGELFKSMANVNLVHVPYKGGGPALIDLVAGHVEIFVAVISTAVPQVK
                     AGKARALAVTGPRRAAALPDVPTVAESALKGYEATNWYGYVVPAATPRGIVERLHKAT
                     VTVLDMSDVKQALLDQGIEAVSSSPEQFTAYIKSETGKWTKVIKATGIRAE"
     gene            11309..12235
                     /locus_tag="AC16CX_03175"
     CDS             11309..12235
                     /locus_tag="AC16CX_03175"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3011545.1"
                     /translation="MFNPFQPLEKIKTEVYTSMPAKFRKKSRTTWSDPNRQGVEVECF
                     LEGPSFDREGNLYCVDIPFGRIFRISPKGEWELVIQYDGWPNGLKFHKDGRAFICDYK
                     KGVLALDPRSGKLEAIIETAFSEGFKGVNDLHFASNGDLYFTDQGQTGIADPSGRVYR
                     LRANSALDRLCDNVPSPNGITLSTTEKHCYVGVTRSQQIWRLPIMADGSVSKTGVAVQ
                     LSGGAAGPDGIEMDAENGLLVCHLGVGIWRFDANMLPTHLVYSDGPHHHHLDNCAFGG
                     PDLKTLYITEALSGDILMAKMPVAGKLMYGLS"
     gene            12485..12679
                     /locus_tag="AC16CX_03180"
     CDS             12485..12679
                     /locus_tag="AC16CX_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011546.1"
                     /translation="MIFLSLGRHLGFLERYLNRTEKENCEADIRDIVFHHLPATLKSI
                     FSKASKDDYLDPKLRDAAGA"
     gene            12768..>13302
                     /locus_tag="AC16CX_03185"
     CDS             12768..>13302
                     /locus_tag="AC16CX_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011547.1"
                     /translation="MTASNTSTEIRFEPPGPGSWEQDPVHFPRPMTRYFQETQPPSFK
                     RGTNDFARFYGMLIDGLQVGYVNGFCYNQVLPAPDAEIPERIQRTQQVFAQKLWRQQL
                     RDWDQNRKPAAIATHRELQAVNPDALSDAELVAYLTRCRDHHSAMIAQHMRFTAGAVI
                     PTGDFLAHVGDWTGLPPS"
BASE COUNT         2452 a         4406 c         4069 g         2375 t
ORIGIN      
        1 gcgacctcgc cgacgtcctt gagcttgccc acgtcccagc acagccggat cagcttgtca
       61 gtctcggccc gggacaggat gccttccgcc agcccgcgga atttggcctc caggtcggcg
      121 tcgctcatcg gatgatcgag gcttcccacc gcatgctcga tgtatttttc gagcgtcttt
      181 ccatttttca atttgatcga gacatgcact tgatcctcgt gcatcgcccg atccaccgtc
      241 gccgtgaccc tgtcccgcag cgcgatcacc ccgggatctc gcaccgcctc gtcgctgtac
      301 tgcgcctctc cggcggcgcc atgaataacc gccacggcgc aggagtggta gacgctgaac
      361 ttgccctcaa ggccggattg aggcgccttc ttgccggtga gttccagcac cagggggtgc
      421 gctttcagag aaatgctctc gatatcgccg gccttcaact ggtgcgcgtt gcgcagctgg
      481 atgcagccgt ctatggccgg gtgctcgacg atgccgcagg cgaatggctt gtaggtgttg
      541 agcgagattt cccacgtttc gccgagcttt cccgtgatct cgccgagatt gcgctcggtg
      601 gcgagcacgt tggcgaaagc gcggcgccct tcgatgccct gctcggaact cgtgaagttc
      661 ctggaagcca gcagcgcggc gagcaggccg ttgcgggcgg cgttgccggg atgcatgggt
      721 ttcaccatgg agccgaacat ctcgcgcaga cccgacgatt gcgttgcggc aatgccgagc
      781 gcccagacca tctgctgctc ggtgagcccg agcagtcgtc ccgccgcggc cgccgcacca
      841 aacaccccgg ccgtggcggt gatgtgccag ccgacgtcgt agtgcgacgg gtaaacggcg
      901 ttgccgatgc ggcactcggt ctccaccccg aggatgaatg cgtgcaggaa ctcttcgcca
      961 ttgaccggct tgtgctctgc cagtgcaagg atggcggagg cgaccgggcc gctgggatgg
     1021 atgatggtct tgagatgcgt gtcgtcgaag tcgaaggtgt gcgaggtcgt gccgttcatg
     1081 agcgccgcga gcatgatgtc aagcctgtcg ccgcgcccga gcacggtggc ctcgcgcggg
     1141 cccgagaact cggaaagcgc cgccagcgcg cgctcaacgg tttcatggcg ggaggcgccg
     1201 accgcgcagc ccacccagtt caggaacgag cgcgccgtct cgtgccggac tttttccggg
     1261 atatcgtcgt agcgatgcct gacgataaat ttcgcaagcg tcctggtaac ttccatagtg
     1321 gaggcaacag gtgatttcga attacccgcg tccatcggct ttcatcggcg gccgaacagc
     1381 taatccacgc gcgcgccgga ttgcttggcg atcttcgcgt atttggcgag ctcggccttg
     1441 atgtactgcg cgaattgctc gggcgtgtcg cccaccggct caatgcccaa ggcctcgaag
     1501 cgctcgcgca cgtcggcgag cttcaggatc ctcaccactt cgccatgctg cttcgtgacg
     1561 atctcccgag gcgtgcccgc cggcgcgagc agcccgatcc aggtgctgat ctcgaaaccc
     1621 ttgaatccgg attcggccat ggttggcaac tcgggcagtc cccgccagcg cttcgccgtg
     1681 gtcatcgcga ccgcgcgcag ccggcccgcc ttgacgtagg gcatgacgga gggcagcgtc
     1741 gcgaaggtga gtgcgacctg cccgcccagc aggtcgccga tgagtgcgcc tccgccctta
     1801 tacggcacgt gcaccatgtc aacccccgca atgatcttga acatctccgc ggcgaggtgc
     1861 gtgccggtgc cggcccccgc cgaggcgtag cttagctgtc cgggcttcga tctggcgagc
     1921 gcgacgagat ccttgaccgt tttcacgggg acagacggat gcaccacgag gatgttgggc
     1981 agcagtcccg gctgggtgat tggcgcgaag tcccgcaccg cgtcgtacgg cacgctcttg
     2041 tagacgctgg gattgatcgc gagcgcgctg ctcgccatga gcagcgtgta gccgtccggc
     2101 gcgcttttcg cgacgatctc agcgccgata ttgccgctcg ccccggcgcg attgtcaacg
     2161 accacctgct ggccccagct ctccgtcatt ttctgcccga ggatgcgcgc gatgatatcg
     2221 gcgcctccgc ccggcgcgaa aggcactacg atccggaccg gtttcgcagg ataggtctgc
     2281 gccttcgcta gaaacgaaaa cgcaagaaga agaaaaacgg ctgcgattcg cagcatgtcg
     2341 gctcctaccg cagatacttg cgttcccact cctcgatttc ggaaagcttc agcgcttcat
     2401 tgtattcctt cagcgtaagc atccggtcgc gcaccgcttt cgtgttgccg tcgcgcttga
     2461 gcgcggcaag cgcctcgcgc atcgcatacc ctgccgcagc gcgctcgagc ccggggtaaa
     2521 tcgcgatgcg caggccgagc cgcccgatcc cttcgacgtc gagctcgtca agcttcccgc
     2581 ccgcgcccat gttgacgagg catggcgcgt ccacctccga agtcacgcgc ttgaggtctt
     2641 ccagtatcga gggcccgcct ttcatctcga cgaagatcac gtcggcgccg gccttgcgga
     2701 atgccttccc gcgcttgatc gcctcgtcga gaccatgggc ctcggcagag tcggtgcgcg
     2761 cgatgatgat gaagtccgga tcccggcgag cggctaccgc cgcctcgacc ttgccgatcg
     2821 cctcgcgcag atcgatcacc ggccgcgctc cagggagctg gccgcagcgc ttcggcgaca
     2881 cttggtcttc gatgtggatc gcggccactc cggccgcctc gaattcctcg accgtgcgcc
     2941 tgacgttgac cgcattgccg tagccggtat cggcgtcgca gatgaggggg atatccacgc
     3001 aggacgcgac acggtgcgct tggccggtga tcagcgtgag atccaccagc ccgacgtcgg
     3061 ggcggcccag caggctcgcg gagacgccgt taccggtcat gtaaaccgcc tcgaagccgg
     3121 cctgctccac gagccgggca ccgtaggcgt cgtaaatccc cggcgcgata acgacgggtc
     3181 cttcggtgag gcgccggcgc agaatccggc gcggcgcttc tttcatgccg cggcttgcct
     3241 gatgtcgaaa cggtatttcg cgagctttgc gtcgtcgaga tcgacgccca gtcccggtcc
     3301 gcccggcagc gggaggctgc cgctggaaag gtcgagcttg cgggtgacga tatcgtccgc
     3361 cgtcttgagc ggccccacgc attccaggcc gtcgatgacg ttgccggaag tcgccgccag
     3421 catgatctcc gccatggtgt tgacccccag cccgaagccg tggcccacca cgcagcggat
     3481 gccggccgcc tccgcggtcg caatcatccg tcgggtgttg agaaaaccgc cctgcttcat
     3541 caccttgacc ttgacgatgt cggcggcctc cgccttgatg atgcggatca agctcgcgtg
     3601 atcgaggacg gactcgtccg ccatgaccgg gaccccgacg gcgttcgcct cgcgccgcac
     3661 ccgcgccatt ccggcgatat cgtgatccgg aaccggctgc tcgaagagtt gcagtttgaa
     3721 cggcgccacc atcctcgcaa gcttgatcga agtatcggca tcgtagcccg cgttggcatc
     3781 tatgcggagc tggaagtcct ggcccaccgc ctcgcgcacc gccttcacgc gatcgcggtc
     3841 ggcctcgatg tcgccgccga ccttgatctt ggcggaacgg aatccccggt tcttccattc
     3901 cagcgtctcc ctcgcggcct ggtctggcgg caggatgccg atccaggcgt tgaacagcag
     3961 cctgtctttc accgccccgc cgagataggc gtgcaccggc atgttcgcca cacgcgccgc
     4021 aatgtccacg cacgccattt caatcgccgc cttcgagtcg agaaaaccct tcacggcgga
     4081 ttcgaccctg gcgagcacag catgcacgtt ggtcgggtcc atgcccatga gcaaaggcgc
     4141 aagccgggtc tgcagcatct gaagatgctc ttccggccgc tccttggaat agccgggcgt
     4201 gggatcgatg ttgcccaggc ccgtcacgcc gccgctcgcg ataatgcgca cgatcgcgct
     4261 cttctgcacc gacttggtga gataggcgtt cttgtactcg ccgatcagcg gcatggcaac
     4321 gccgaacact tcaacgcgct cgattttcat cggagcctag tgattaagtg attaagtgat
     4381 taggtgatta agcgattaag aaaatgcacg ctggcgctac ggattgtcaa acgctcaatc
     4441 acccagttac tcagtcactt aatcactgcg acgagagtcg ccattcggga tgcgctttca
     4501 gaaagggaat caaccccccc gcggcgagta tagcttggac ttcgcctggc agcggcgaca
     4561 cctcgcgcac gatgccgcgt cccggtaccg cgacgcgccc tcctgccagg tcgatctcga
     4621 tctcatcgcc ctctgcgaca ccggccatat cgcactcgac gaccggaagc ccgttattga
     4681 tgcagttgcg gaagaactgc cgtccgaagg aagccgcgac gatcgccgac acgcccatca
     4741 gacgcatgat gtgcacggcc tgctcgcgcg aggaattgtt tccgaagttc ctcccggcga
     4801 cgatgaggtc gccgctcgcg aaacgggcgg cgaagcccgg cgccagctgc tcgaaggcgt
     4861 gctgcgccac gtaagcgttg tcccggcccg gatggtactt gctcgagcaa tggacgtcgg
     4921 tattgatgtc gtcgccgagt agatgcgcct tgcctcgtat aaatgtcgtc atgtgcggtc
     4981 accaatcacg agtcgcgagt cacgagtcac gcagctttgg aaatgggccg ccattctcca
     5041 cccgccacag ttcgcggatc cgttatacat cccgtgagcg ctgacgctgc cacagtggca
     5101 gccgatgcga tgaagatgtc ggagtccgga ttgccgagcc gccccttgaa gttgcggttg
     5161 gcgctcgaga tcaccacgtc gccgtcgccg gtgatcaggt gacccgtgat gttgacgcag
     5221 gtgccgcagc ccgccgcctc gacagaggcg cccgcctggg tcagggcttc gatcagtcct
     5281 tcgcgcgcgg cttgcaggta cacattgcgc gacgcgggtg tgacgatcat gcgcacgccc
     5341 ttcgcaatgc gcctgccctt gagaatcgcc gcggcctggc gcaggtcatc cagccggccg
     5401 ttggtgcagg tgccgatcag cgcatagtgg acgcgctggt ccttgatttc cgtcacgcca
     5461 accacgtcgt caatgcggtg cttgcgcgcg acctgcggcg ccagccgtga agcatcgatc
     5521 tcgatgcgct cgacataacg tgcgtcggga tcggccttga ccggttgtgg gtcgcgtgcg
     5581 ccgcgcgcct tgagccaggc gagcgtcttc tcgtcggcct ccagtaacgc cgccttcgcg
     5641 ccgagttccg ccgcatggtt ggcgagcgtc atgcggtcgt cgatctccat cgcggccacc
     5701 gccatgcccg cgtactcgat cgccttgtag ttcgcgcctt ccgcgcccag ccgtccgagc
     5761 atgcgaagcg tgatgtcctt tgcccacacc cccggctgca gccttccctt gagatccacg
     5821 cgaatggtct caggaacacg gaaccacagc tttccggtca tcaacaccgc cgtcagatcg
     5881 gtgccctcca cgccggtgcc gaaggcgttg aacgccccat aggtggtcga atgcgtgtcc
     5941 gtgccggtta cgaggtcgcc gcacgtaaga tgcccgccct cgggcagcac ctggtggcag
     6001 atgccgtcgc cgacgtcgta gagctgcacg ccgtgctctt cggcgaacgc ccgcatcgcg
     6061 cgatgtatgt tggccgcctc cggatgaggc ggcggggagt aatgatccag cacgaaggcc
     6121 gtgcgcgcgg cgaacggcag cttcgcggag ccgaactgct cgatgcgctt caacgcattg
     6181 ctcgctcgcg cgtcgtgcaa catcgcgtag tccacgcggg cgaccacgac ctctcccgct
     6241 tttgcggcct tgccgctggc gtgcacaccc aggatttttt ccgcgatcgt tgcgcccatg
     6301 cccgtccctc tcatcgagcg gataggcatt ttacaggctt cccggataat tttccggcga
     6361 cggcctcgga taggagatcg cgagggacag caactcaagt cggccgcttg cgcgcctccg
     6421 cggtcgcacc cgcaagcggg cacctgctcc acgctccggc gcgctccccc ctgtggcatg
     6481 aaggcgcgag atgtgatatt taagcggctg cccatccact gccacgaaca tgcctgccgc
     6541 gccggaaatt tcactgacgt cctacgtcgc cgaattcatc accatgacca aggcttccgg
     6601 cattcccgcc gatgtcatgc atctcggcaa gcgctcgatc ctcgacggca tcgggctcgc
     6661 gctcgccggc aacgccgccg agtcgggaca gatcgtgcgc cagtacctga aatcgctcgg
     6721 ctgcccgacg gacaagggct gcacggtgat cggcacgaat ctcaaggtcc cggcgcgctt
     6781 tgcggccttc gccaacggca tcgcgatcca tgccgatgat tacgacgaca cccagctcgc
     6841 ggtggccagg gaccgggtct acggcctgct cacgcacccg acggcgccgg cgctgccgcc
     6901 ggtgctggcg ctgggcgagc tcgccaacca tccgggatcg gacgtcctgg ccgcctacca
     6961 ggtcgcggtc gaggtcgaga ccaaggtcgc cgaggcgatc aacccgcgcc actacgacga
     7021 cggcttccat tccactgcga cgatcggagc gatcggcgcc gctgccggcg cggcgcggtt
     7081 gctggggctc gacaccgagc agacacagcg agccctgggc atcggcgcga gccagtccgc
     7141 aggcttgcgc gagaatttcg gcactatgac caagccgttc catcccggcc gttccgcgga
     7201 aagcggcgtc gtcgcggcgg aattcgcgaa gctcggctgg accgcgacgc ccatcgttct
     7261 ggaagccggg cgcggcttct tccaggcggc gggcggcggc tacgacacga aagcgatcga
     7321 gggcaagctc ggcagcccct ggacttttgc cttccccggc gtctcgatca aaccgcatcc
     7381 ttcgggctcg ctcacacacc ccggcatggg tctcatgctc gatctcgtcc gcaagcacga
     7441 tatccgtccg gcacaggtcg tgaaggtgaa ggtcggaacc aaccgccaca tgccgaacgc
     7501 gctgatccac caccgcccca aaaacgagct gcaggccaag ttcagcatgg aattctgcat
     7561 ggcgatcctg ctgctggagc ggaaggcggg actggccgag ttcaccgacg aggtggtgaa
     7621 ccgccccgac gtgcggaaga tgatcgaaaa ggtcgagttc agcgtgcatc ccgaagccga
     7681 ggctgcgggc tacgagaaga tgaccacgat catagacatc gagctcaccg gcggcaggag
     7741 gctcggcggc cgcgccgact tcggcaaggg cagtcccgcc aatccgatga gcgatgacga
     7801 gctcgccgac aagttccgcg aatgcgccgc ctggggcagg ctgccgaagg cgaacgccga
     7861 gaaggtgatc gatctggtct tcgatctcga gaagctcaag agcatccgcg aactgacgcg
     7921 cttgcttgca gtcggccgca aacgcgtcaa atgaggaatt aacgtgagag cgcaagagcg
     7981 ggagagcgtg agggcgaaga gaaattctat ccaagctccc atttcacgat ctcccgattt
     8041 cacgatctcc cgacttcatg agatgacctc cgttaccagt cagagtcgcg caacatgaag
     8101 aaagaccttc ccgtgatcgg cttgatgctc ggagacatga ccggcatcgg gccggagatc
     8161 tgcgccaagc tgattgcctc cggaacaccg ggcgaagtcg cgcgcgtcgt cgtcatcggc
     8221 gacgcgcggg tgctcgatct cggcatgcgc gatgccgggg tcaagctggg ctatcgcatc
     8281 cactccgggg tggatgacgt gcgttggccg gtcgaggcgc tgccggtggt ggacctcggc
     8341 aacatcgacc cgtcgcgcat caagcggggt gaagtctccg ccgaatccgg cagactgact
     8401 ggtgaaacgt tgaagtacat gatcgacctc gcgctggccg gcaagctcga tggcatctgt
     8461 ttcgcgccgc tcaacaaaat ggcgctgcac cagggcggat ggaaattcca cgacgagcac
     8521 cagatgttcg cgaaactcac cggccacagc ggcttcttcg gcgagatgaa cgtgatcccg
     8581 gaattcagca ccttccgcgt cacttcgcac atcgcgctgc gcgaggccgt gaatctgatc
     8641 tcgccggaac gcatcgccgc cgcagtgcgg ctcgccgaca ccacgctgcg cgcgatgggg
     8701 caccggcggc cgcggattgg cgtcgcggcc ctgaatccac acggcggcga gaacggactg
     8761 ttcggcgacg aggagatccg catcatccgt ccggccgtgg ataaactgcg cgaaagcggc
     8821 atcggttgca tcgggcccat cccctcggac acgatattcc tgcgcgcctt gaagggccag
     8881 ttcgacggcg tggtgatgat gtaccatgac cagggccaga tcgcgaccaa gctgatcggc
     8941 ttcaacaagg gcgtcaccgt caccgcgggg ctgaagaccg tgttcgccac tcccgcccac
     9001 ggcaccgctt tcgacatcgt cggccagggt cgcgccgact acaacgcatt ggaatatgcg
     9061 tttagactag cagccaggct cgcatccaat accggcaggg gagtcggcgc gacctgattg
     9121 acaatcatga tgattaattc cgctcttccg aggagaccaa gataatgaaa atatcgattc
     9181 atggaatggt tttcaattca atctgcggca tgctcgttgc cgccgcccta ccgagtaccg
     9241 ctgcgcgcgc cgcagattgg ccgaccaagc ccatacgcct cgtcataggg ttcgcgccag
     9301 gcggcggcac ggataccacc tcgcgcgccc tcagcacgaa gctgacgacg tcattcggcc
     9361 agcaggtaat cgtggacaac cgccccgggc attcgggcac catcgccgca gacatcgtgg
     9421 cgaaggccgt ccccgacggc cacaccttgc tgctgggtac cattgcgctg gtgatcaacc
     9481 ccagcctcac cgtgaagatg ccgttcgaca ccctcaagga cctcgcgccg gtgacgcgcg
     9541 cggtggactc gaccaacata ctcgtggtgc atccgtcggt cgccgcgaag tcggtgaagg
     9601 agctcatcgc cctcgccaag acgaagactc tgaatggcgg ctcttcgggc atcggcggca
     9661 ccgggcatct ggcggtggag ctattcaacc tccaggccgg cacgaagatc acgcacgtgc
     9721 cgtacaaggg cggcggtccc gcgatcgtgg atctccttgc aggcaacatt caattgatct
     9781 tcgccacggc ggcgagctcg atcgcgcaca tcaacgccgg caagatccgg ccgctcgccg
     9841 tgaccacggt gaagcgttcg gcgctggtgc cggagctgcc gacggtcgct gaagcggggc
     9901 tgcccggttt cgaggcgaac aactggaacg ggttcctggt ccctgcgaaa acgccccgcc
     9961 ccatcatcaa ccggctcaac aaggaattga cggccgcgct cctgtcgccc gacatcaagg
    10021 agttcctgtt caagcagggc ctcgacgcgg cccccgacaa tcctgaagca ttcggagcct
    10081 atatgaaatc ggaaatggca aagtgggcga aggtgatcaa ggccgccggg atcaagccca
    10141 attgagaacc ggtgataggt gacgagtgat gagtgatgaa aaaccgttga gagggctcgc
    10201 gcacgcggca atcgtgtgcg tgtcacttgc catagccgcg ccaatcgctg cgcaggagta
    10261 tccgaccaag ccggtgcgca tcatcatcgg attcccgccg ggcggcgcta ccgacctcgt
    10321 ctcccgcctg atggcgccca agtacgccgg catcttcaag cagcaattca tcgtggacaa
    10381 ccggccgggc gcgaacggcg tcatcggctc cgacctcgcc gcaaaggccg cgcccgacgg
    10441 ctatgcgatc catctcgcga ccatcggctc gctggtgttg agtcccgcta ccggcaaggt
    10501 gccgtacgac ccgatcaagg atttcgctcc ggtctcgcag gcggtcgcgt tgcagaacat
    10561 cttcatcgtt caccccacct tgccggcgcg cacgatgaag gagctgatcg ccctggccaa
    10621 ggcaaggccc ggcaagctca atttcgcctc ctccggaaac gccagcccgg ggcacctggc
    10681 aggcgaattg ttcaagagca tggcgaacgt caacctggtc cacgtgccgt acaagggcgg
    10741 cggccccgcg ctgatcgacc tcgttgccgg ccacgtcgaa atcttcgtcg cggtgatctc
    10801 gacagcagta ccccaggtca aggcgggcaa ggcacgcgcg ctcgccgtca ccggtcccag
    10861 gcgcgcggcg gcgctgcccg acgtgccgac ggtcgccgag tcggctctca aaggctacga
    10921 ggccaccaac tggtacggct acgtggtgcc ggcggcgacg ccccgtggca tcgtcgagcg
    10981 cctgcacaag gcgaccgtca cggtgcttga tatgtccgac gtcaagcagg cgctgctcga
    11041 ccagggcatc gaagcggttt ccagctcacc ggaacagttc accgcgtaca tcaagtccga
    11101 aaccggcaag tggaccaaag taatcaaggc gaccggaatc agggccgagt gacggctgac
    11161 gactgaccgt gagagcggga gagcgttaga gcgcgagagt gaacattcga tttcccgatc
    11221 ccacgatctc ccgatttcac gcataagtcg acccacgatc cacgattcac gatccacgat
    11281 ccacgatcca cgatccacgg catccaacat gttcaacccg ttccagccgc tcgaaaaaat
    11341 aaagaccgag gtctatacct cgatgcccgc gaagttccgc aagaagagcc gcaccacctg
    11401 gtcggaccca aaccgccagg gggtggaagt ggaatgcttt ctggagggcc cctctttcga
    11461 ccgcgaaggg aatctctact gcgtggatat tccctttgga agaatcttcc ggatatcccc
    11521 gaaaggagaa tgggagctgg tcatccagta cgacggctgg cccaatggac tgaagttcca
    11581 caaggacggg cgcgccttca tctgcgacta caagaaaggc gtgctcgccc tcgaccccag
    11641 gagcggcaag ctggaagcga taatcgagac cgctttcagc gaaggcttca aaggggtgaa
    11701 cgacctgcac ttcgcgtcga acggcgacct ctatttcacc gaccagggcc agaccggcat
    11761 cgccgacccg agcggacgtg tctaccggct gcgcgcgaac agcgcgctcg atcgattgtg
    11821 cgacaacgtg ccgagcccca atggcatcac gctttcgacg accgagaagc attgttacgt
    11881 cggtgtcacg cgctcgcagc agatctggcg gttgccgatc atggccgacg gctcggtgtc
    11941 caagaccggc gtcgcggtcc agctctccgg cggcgcggcg ggaccggacg gcatcgagat
    12001 ggatgcggag aacggcctgc tcgtgtgcca tctgggcgtc ggcatctggc gcttcgacgc
    12061 caacatgctg ccgacccatc tcgtctactc ggacggcccg catcaccatc atctggacaa
    12121 ctgcgcgttc ggcggacccg atctcaagac gctctacatc accgaggcac tgtccggcga
    12181 catcttgatg gcgaaaatgc ccgtcgcggg aaagctaatg tacggcctca gctgagatca
    12241 ccctccgtcg acctccggaa cggctggata cggccacaag cggtcattgg cagaccggct
    12301 attcgatcac ccagttggcg cgcaggaaga ttgactgtgg aatctttatg ccgagcgttt
    12361 tggcggtctt gacgctaacc accagctcga acttggtcgg ctgctcgatc agcagatcac
    12421 atgggttagc gccttttctt ccataaaggg gcgcgcgatg gattgccgcg tccggagggc
    12481 cagcttgatt ttcctgtcgc taggccggca tctcggattt cttgaacgct atctgaatcg
    12541 aactgaaaaa gaaaattgcg aggctgatat ccgcgacatt gtctttcatc acttgccggc
    12601 cacgctaaag agcatcttct cgaaagcgtc gaaagacgat tatctcgacc cgaaattaag
    12661 agatgctgcg ggggcctgaa cgggcacctt gccctccgtt ccaaacgagc aaataatagc
    12721 cagccgcgcc gtgagatctg cgggccgtcc cacagaaagg aagcgccatg actgcgtcaa
    12781 acacctcgac cgaaatccgt tttgagccgc cagggccagg atcgtgggag caagaccccg
    12841 tgcacttccc gcgcccgatg acgcgctact tccaggagac gcagccccct tccttcaaga
    12901 gaggcaccaa cgacttcgcc cgcttctacg ggatgctcat cgacggcctc caggtcggct
    12961 acgtgaacgg cttctgctac aaccaggtgc tgccggctcc ggacgccgaa atccccgagc
    13021 gcattcagcg cacgcaacaa gtcttcgcgc agaagctctg gcggcagcag cttcgcgact
    13081 gggaccagaa tcgcaaacca gccgccattg ccacgcaccg ggagctgcag gccgtcaatc
    13141 cggacgccct ctccgacgcg gagctggtcg cctacctgac gcgctgccgc gaccaccatt
    13201 cggccatgat cgcccagcac atgcgcttca ccgccggtgc cgtgatccca accggggact
    13261 tcctcgcaca cgttggggac tggacgggcc tgccgccgtc gg
//
LOCUS       DBNGWZ010000106         1944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1269301, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000106 DBNGWZ010000000
VERSION     DBNGWZ010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1269301"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16CX_03190"
     CDS             complement(<1..470)
                     /locus_tag="AC16CX_03190"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3011548.1"
                     /translation="MSVRAVLVGFLAAACAAMSVASFGQLQTFPSRPVRVIIPFPSGG
                     SVDVVMRIFSNRFAELTGQNVIIDNRPGASGNVGTDLAARAAPDGHTVLATTLPLVVN
                     PSLFGNLNHDVLRDFAPVSLLAAAPFVLVVHPSLPPGSVKELIAYARVRPGELN"
     gene            605..1582
                     /locus_tag="AC16CX_03195"
     CDS             605..1582
                     /locus_tag="AC16CX_03195"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR3011549.1"
                     /translation="MKAVEISRPGGPEVLTPVERPVPEPGPDEALIKVAATGVNGPDL
                     MQRKGLYPAPPGASDLPGLEVAGEVVATGAKVKRWSVGDRVAALTNGGGYAEYCAVDA
                     QHCLPIPKGVGLIDAAGLPETFFTVWSNVFMGAGLSAGETFLVHGGAGGIGTTAIQLG
                     KAFGAKVIATDSPAERCAICRDLGADRAIDYRQEDFVEIVREAGGANVILDIVGGPNI
                     EKNFKAASHDARIIQLAFALGSKVDVNLMPVMLKRLTYTGSTLRTRPPAYKARIAREL
                     EAKVWPLIESGRIRVVTRQVFPLAEAARAHALMESAQHTGKILLTVGEA"
     gene            complement(1659..>1944)
                     /locus_tag="AC16CX_03200"
     CDS             complement(1659..>1944)
                     /locus_tag="AC16CX_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011550.1"
                     /translation="DGSEEIVFASAADLIFKTPRFYQGATKRLKQDLGGCYNYIEEYF
                     GGQNLYFDELEKNINHARAIAVEGDISLLRRRPPNTTERSEPDQPQQLLI"
BASE COUNT          355 a          618 c          655 g          316 t
ORIGIN      
        1 tagttgagct cccccggcct cacgcgcgca tacgcgatca gttccttgac cgacccgggc
       61 ggcagcgacg ggtggaccac cagcacgaac ggcgcggccg cgagcagcga caccggcgcg
      121 aaatccctga ggacgtcgtg attcaagttg ccgaacaggc tcggattcac caccagcggc
      181 agggtcgtcg ccagcacggt gtgcccgtcg ggagcggcgc gcgccgcgag atcggtgccg
      241 acgttgccgg aagcgccggg gcggttgtcg atgatgacgt tctgccccgt cagttccgcg
      301 aaccggttgc tgaaaatgcg catcacgacg tccacgctgc cgccggaggg aaacgggatg
      361 atcacgcgca ccggccggct cgggaacgtc tgcagctgcc caaaggatgc gacgctcatt
      421 gcggcgcagg ccgccgccag aaagcccacc aggaccgctc taaccgacat actcctcctc
      481 gttcgtgcaa ttccgcgggc gtgtgcgccg ggaattcgga attttctgaa tgttagtata
      541 gagcctgacc tgacgcccga aaatcgcaat ccaatacgaa atgagcggaa acctgccgaa
      601 acagatgaag gccgtcgaaa tctcccgacc cggcggtccc gaggtgctga cgccggtcga
      661 gaggccggtg cccgaaccgg ggccggatga agcgctgatc aaggtggcgg ccaccggcgt
      721 caacggtccg gacctcatgc agcgcaaggg gctctacccc gccccgcccg gcgcctcgga
      781 tctgcccggt ctcgaagtag ccggtgaagt ggtcgcgacg ggcgccaaag tcaagcgctg
      841 gtcggtcggc gacagggtgg ccgcgctgac caacggcggc ggatacgccg agtactgcgc
      901 cgtcgatgcg cagcactgcc tgccgatccc gaagggggtc ggattgatcg atgcggcggg
      961 cctcccggaa accttcttca ccgtgtggag caacgtcttc atgggcgccg gcctctcggc
     1021 gggagaaacg ttcctggtcc acggcggcgc gggcgggatc ggcaccacgg ccatccagct
     1081 cggcaaggcc ttcggcgcga aggtcatcgc gaccgacagc cccgcggaac gttgcgcgat
     1141 ctgccgtgac ctgggggccg accgcgccat cgactaccgg caggaggatt tcgtggaaat
     1201 cgtgcgggag gccggcggcg cgaacgtcat actcgacatc gtcggcgggc cgaacatcga
     1261 gaagaacttc aaggccgcaa gccacgatgc ccgcatcatc cagctcgcgt tcgcgctcgg
     1321 ctccaaggtg gatgtcaatc tgatgccggt catgctgaag cggctgacct acaccggctc
     1381 caccctgcgc acccggccgc cggcctacaa agccagaatc gcgcgggaac tggaggcgaa
     1441 ggtctggccg ctgatcgaga gcggccgcat ccgggtggtc acccgccagg tctttccgct
     1501 cgcggaagcg gccagggcgc acgcgctgat ggagtcagcg caacacaccg ggaagatctt
     1561 gctgactgtc ggtgaggcgt gacgagtgat aggtgatagg tgctaaggcc ggcgatgccg
     1621 gcccttctta tcacttatca cttatcacgc tgttgccgct agatgagcag ctgctgcggc
     1681 tggtcgggct cgctgcgctc ggtggtgttg ggagggcgcc ggcgcagcag ggagatgtca
     1741 ccttcgacgg cgatggcgcg cgcgtggttg atattttttt cgagctcatc gaagtacagg
     1801 ttctggccgc cgaaatactc ctcgatatag ttgtagcagc cgccgagatc ctgcttgaga
     1861 cgcttggtcg ccccctggta gaaacgcggg gtcttgaaga tcaggtcggc ggctgaggcg
     1921 aaaacgatct cctcgctgcc gtcc
//
LOCUS       DBNGWZ010000107        14272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1899402, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000107 DBNGWZ010000000
VERSION     DBNGWZ010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1899402"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16CX_03205"
     CDS             complement(<1..712)
                     /locus_tag="AC16CX_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR3011551.1"
                     /translation="MKHELELGTLESGRNFTLPLDFVTRTLAIIGIRGWGKTVTATVI
                     AEEMCECGIPWIAFDPTSVWWGLRCNPDGTPGGYPVLVVGGPHGDLPLEKDEGPRIAE
                     AVLQENLCCVIDLKLESKNTWRKFVADFCDRLMELTPAVPHHIFLEEAPEFVPQRPMG
                     EQKRSLAAVDRLSRLGRNNGYGATLISQRYATVQKDVLTMCENLIAGLTIGKTDRDAM
                     KGWIEEVVQVTESGKKADE"
     gene            complement(709..972)
                     /locus_tag="AC16CX_03210"
     CDS             complement(709..972)
                     /locus_tag="AC16CX_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011552.1"
                     /translation="MSLTPSLQRKAVEALASRQCACGGFKERKHAFCIRCYAALPLEV
                     KRKLWRTFSEGFAEAYDEAVDWLRVHTDRLTQGGLRFVDPEIQ"
     gene            complement(969..1148)
                     /locus_tag="AC16CX_03215"
     CDS             complement(969..1148)
                     /locus_tag="AC16CX_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011553.1"
                     /translation="MNADACWCCGEPLDLEGECPEGCCDGCEECCHRPQCCVCYNPDG
                     VDGEEDEFVEEDEDA"
     gene            complement(1145..1372)
                     /locus_tag="AC16CX_03220"
     CDS             complement(1145..1372)
                     /locus_tag="AC16CX_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011554.1"
                     /translation="MCDLCHQNGDEQMSCQDCGRFICFDVKSGDDVLRPAYVTGSGDL
                     FCDLCGRQHDEEEEREEALDYCEPPEDLEVG"
     gene            complement(1401..1643)
                     /locus_tag="AC16CX_03225"
     CDS             complement(1401..1643)
                     /locus_tag="AC16CX_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011555.1"
                     /translation="MRRKTEAGQCRFCGCTETTACTIFRIVVRWNCLASFHQTTCTWA
                     DASRTVCTNPDCLRLREGARHPVIRRELRRARESKR"
     gene            complement(1640..2128)
                     /locus_tag="AC16CX_03230"
     CDS             complement(1640..2128)
                     /locus_tag="AC16CX_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011556.1"
                     /translation="MSDTVGVEGRVWSPNWSPRIPWCFFEELSKNGWRPDTKWAEGLD
                     WKQIGPQCVHVLEPLPINPSDWWIEPYCCLAGTVVFYSTYAASHREVARCDGPYRMWP
                     NISFVERGGLLYEVCSGKVVHVFDRWQHEWRAFKNTEVARHFNGICGGCKYCDLLREE
                     AA"
     gene            complement(2145..2606)
                     /locus_tag="AC16CX_03235"
     CDS             complement(2145..2606)
                     /locus_tag="AC16CX_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011557.1"
                     /translation="MPEAMQFALGWTERLEEIERRLEAAMRSSRLGPFERDVLAVIAM
                     HRGCRNPVASESIAAQLHMEWCENSRRAIAGAVENLVLLEKVPIGGRRGKPHGYFLVM
                     TAEDLDLAIHARWSEVYAHLRYLRALTSKSDVARLFGQGMLQLDRDGKEAA"
     gene            complement(2606..3604)
                     /locus_tag="AC16CX_03240"
     CDS             complement(2606..3604)
                     /locus_tag="AC16CX_03240"
                     /inference="COORDINATES: protein motif:HMM:NF019146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HIR3011558.1"
                     /translation="MGDRSRIEWTDATWNPIRGCSRVSEGCRNCYAEREAARFSGPGQ
                     PYEGLAEVRPAGPRWTGEVRLVESALEVPLRWKKPRRIFVNSTSDLFHEKLREDWLHS
                     IFQAMVEAPQHIYQMLTKRVNRMRDVLQQMRKSWSLPSMRGCPGDLSAAPNIWWGVSV
                     EDQKTADERIPVLLDTPAAVRWVSAEPLLGVVSMCRLQGNLGMKCDLVMDALRGEYVP
                     AWKGRDTIPDTRCGKRLHWVVVGGESGPNARPMHPDWVRSLRDQCEAAGVPFFFKQWG
                     EWCPESQLTDYELATRKSPVRRNGDFWRTGKKRAGRLLDGREWNEYPASADPKMRV"
     gene            complement(3604..3816)
                     /locus_tag="AC16CX_03245"
     CDS             complement(3604..3816)
                     /locus_tag="AC16CX_03245"
                     /inference="COORDINATES: protein motif:HMM:NF048408.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR3011559.1"
                     /translation="MAMECHRCGERWTVHPALTVACPECQAAAGRACRRPSGHAIFGG
                     EVHVAREQLAVDRGLMTKCRGEGRSE"
     gene            complement(3816..4208)
                     /locus_tag="AC16CX_03250"
     CDS             complement(3816..4208)
                     /locus_tag="AC16CX_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011560.1"
                     /translation="MNGEGMCQWCFAGRPHPSDVAAQRQQQKIAEGKAKFEEATMRPP
                     TPEAVKVQVRRDRAAGMTHKAIAEKHHLSLSNVQRIAAGGNGQRSDQRPPKNGAITIV
                     ATPELVDGIWRGLPLERKAALINRLSEV"
     gene            complement(4567..4824)
                     /locus_tag="AC16CX_03255"
     CDS             complement(4567..4824)
                     /locus_tag="AC16CX_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011561.1"
                     /translation="MSGQRSALLDRIVDLEMAGAIESWGFLGDAVQVMFVKAAECCHC
                     RTMHFYFVNRDGRTRCVECDDTYLREREMLRPNLTTAGAGR"
     gene            complement(4821..5162)
                     /locus_tag="AC16CX_03260"
     CDS             complement(4821..5162)
                     /locus_tag="AC16CX_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011562.1"
                     /translation="MSWALLVKAAEALVLLILAIVAGAIGLVVAAICIGFALWAVLWF
                     ALYISSAARVWRMQGLHPRVRFERATAQFQSGKMSIGAYRDEIVRWEAWRQHAEGWQQ
                     TLGYAPKGRPN"
     gene            complement(5159..5533)
                     /locus_tag="AC16CX_03265"
     CDS             complement(5159..5533)
                     /locus_tag="AC16CX_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011563.1"
                     /translation="MNEQLRDAQENTVEDRFAGIRDFFLDNLHLAGLSRTELYNRPKP
                     GELREPTEEEIVRRWAELVAAPLGDIFIGIQRAFAFAAERGQIVTSFRYCVPQIQRRL
                     AEVREARAGLGPRMARMKERQT"
     gene            complement(5530..5997)
                     /locus_tag="AC16CX_03270"
     CDS             complement(5530..5997)
                     /locus_tag="AC16CX_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HIR3011564.1"
                     /translation="MNWGGTTRVATAATVVAALLAVPLRGQEAIKFHLGKEQPVILLK
                     VKVNGVERVALLDTGGSRTITTPAAAGMSKKDLGASPLTTLGGGKTAMAYRAVRLEFG
                     GKRWSGLTVIVTDLQAVSENVGEPVELVLGMDVLSQFHAFRVDFRKGVLELEP"
     gene            complement(5994..6476)
                     /locus_tag="AC16CX_03275"
     CDS             complement(5994..6476)
                     /locus_tag="AC16CX_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011565.1"
                     /translation="MAEIKTLAAWKIDQVAVLYLGAQAGLEVASREASARRARLIELT
                     REYGSTAPRAWKTKRLCGSAYEVRVSEAQEITVDMRAAMRIKYACIRSGTGRLFRRLF
                     RKVETFILAPGAERLLDQGRLPEDAPRNLRALFARAVRVRELAPHVEVGKREKPEEAA
                     "
     gene            complement(6469..6795)
                     /locus_tag="AC16CX_03280"
     CDS             complement(6469..6795)
                     /locus_tag="AC16CX_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011566.1"
                     /translation="MRRGLKRIVGALRGAVGLCPTWHGSLVRRRGPGPDGRMGFECLD
                     CFTFLPLSDELLERSAIALRMARQQRRLVAHRRARRASRDVAELERLYQLSPVSPARK
                     KDERHG"
     gene            complement(6792..7031)
                     /locus_tag="AC16CX_03285"
     CDS             complement(6792..7031)
                     /locus_tag="AC16CX_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011567.1"
                     /translation="MNGEQWQRHKVRCIDELRPEYVDAAEARRRRLARQQFWARVYAD
                     RERRNRLFSWGAALLCAIAGIVLAMIALDLLGWIR"
     gene            complement(7028..8146)
                     /locus_tag="AC16CX_03290"
     CDS             complement(7028..8146)
                     /locus_tag="AC16CX_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011568.1"
                     /translation="MSAALKGGTYPIAVKRELLVLETPPYEEARREVNRWMVRRGLTA
                     QSAAELLGIARSTLNQWVQGYYPRYHPCNAELLTARIWSEVAKHPAPPPFGPPARFLE
                     TRNSAEIRRLVNACLRQHACAVVHGAPAEEKTFAVKCLIAEHQTAGRDDILYIYAMPN
                     PSPLGLLQQIVSAAGVPVRSNLREPLMRALIAEFRSRERMPCVVVDEAQHLLRRPYDA
                     LETLRRLRDLTANDLAENRRDSYGCGLLLIGSHDLYQRFERDKFLLAQWRDRIQHRKQ
                     LTGMSDSEALEIAARELGNGRPARLGEDLKKTILEFAREMDPYTQICASCRQPVEAGR
                     ELCSCGARTSPAIFYSPRKLSIWIDRAREKRSAQRGVA"
     gene            complement(8143..10209)
                     /locus_tag="AC16CX_03295"
     CDS             complement(8143..10209)
                     /locus_tag="AC16CX_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIR3011569.1"
                     /translation="MYLTIREICALEKLTDRRVRQKAKAEVWESQLSEGRTGNRKRER
                     CYPLTRLSPAAQLRYAEQQVSQATAIVPAARTLPLFAAAPEAVVEARRNDLTPAEKAV
                     ADARYLAIAPLLDFRRRTNGTRPKIQAGDGRTIETLADLAAYVGAQQAPPVSTSTLFR
                     WLRRYETKGGWAGLSDPPRRDKGTSRFFLRHPNAKVFVRAKGQSLKEDLSVQMIWEAL
                     RREWPNLRERGAPPSYSTVAEYLRGVPRPLAVLAKEGPQAYERTCSPFILRSNVPAMD
                     WWVADHRVFDVQVSNALFPWVPPGAAYRMWLTAVYDWGSRTLVGFCFAPRPNSATINS
                     ALRMAALQYGFPRNLYWDNGKDFQKAGAIEEEVRLSENAQRLLRDRCLEVTYALPRHP
                     RSKPIESWFTRWSKRFDRLWHESYLGNSPGACPEIARMSQVLHRKYLQGKRAESPLPM
                     DVIFIVAAVQWIEEYNRTPLESLGGRTPAEVLDEQWPAESRPKIEPRELDMLLEACER
                     TVLPGGCVEIDRLRYEPTDQSLYALDLRQGQRIRVLRDPYDLTQAVARDPETRQFVGE
                     LRLQQLVAQGAHGQISREDINAAMGRQRRPKRAYSEYLGALAAISTRLGWQTELEALL
                     ERAGIQRAQLHTGTDGAGSLPAPKGAPGARLALPERSPLPAIRTFAADVSEEDAALLD
                     QVEVEE"
     gene            complement(10193..10900)
                     /locus_tag="AC16CX_03300"
     CDS             complement(10193..10900)
                     /locus_tag="AC16CX_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011570.1"
                     /translation="MKYWPIVVPNAALFILLCRMAAVGLAASRPAVFGFLCVWLIFSL
                     GTLIAGQTADPAGAGYANFYLAGLILSGAVLTPALWRASAVALRHPAWCILLSTTAIS
                     LSVALGLGLVRLTGPTPAWRALIATTFGSLAAAAIFFCAAMHAAAPDRLLWQGLGWYL
                     LVFGAGSALARLEGRTHAAYFVVAALATIVWLVLAWRVGPHPDVLISLEKLALIPFAF
                     LRRAHVRGNCVRSVPDN"
     gene            complement(11217..11570)
                     /locus_tag="AC16CX_03305"
     CDS             complement(11217..11570)
                     /locus_tag="AC16CX_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011571.1"
                     /translation="MSDFLHLPMMSLDLRKPITVRLEPELAAQAEDRARELERKAGPF
                     CAWCVRQVLRLEFLSPENREFLGGILREVGRPWGPLDLVDGIISIFRKEVRAGRLRPL
                     FWSGQVSNQAKAGPR"
     gene            complement(11567..11797)
                     /locus_tag="AC16CX_03310"
     CDS             complement(11567..11797)
                     /locus_tag="AC16CX_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011572.1"
                     /translation="MSAKRARLQSTTAQIPQPIRKVLLYRGLIARVARQLGLSHTQVT
                     RVARGERTSRRVIAALLAEIERIERESSKKAA"
     gene            11843..12247
                     /locus_tag="AC16CX_03315"
     CDS             11843..12247
                     /locus_tag="AC16CX_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3011573.1"
                     /translation="MPKFSTGQIVRKIRGRLSQQKFADRLGVSIGALRKWESNKARPC
                     PENLDHLAQIAPPELAAHLGIRDTPGSPARTDLAKQVDALEARIRAVEEVLHLPEPRG
                     RAETLAVEGRASPKRKSGTSPAGGVTNHLTGT"
     gene            12613..13794
                     /locus_tag="AC16CX_03320"
     CDS             12613..13794
                     /locus_tag="AC16CX_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011574.1"
                     /translation="MKPTQILLTVLALAVSTVVVTPLAAQSTKVVSRTASSAVFRQDK
                     TAVWTALMEVLADLNIHPERIERNNGLVFFRTEATWADSWGGVNTAVRTMTTKIVSGN
                     SSWQAFIASGNIFCKELEENRTEVRARFQFAAYNGWAGWLGGAGAGWQQLESNGWLEN
                     EILARMEKALPEPMRLAARDAEAIAGAQTVLRLLRKVDASFKIDATGDSLTALLVETV
                     AAADELRAGYKGRLPGAFFDLLSKSLDAFAAARKPDAAVAEHLAQARIAIDNAADQLA
                     ALIEPPSPPAAPSLDLPAGPPPAFYEDRLTVPAEACPEGFRIAIAPRGMQVPITSIAM
                     DGGGFDYVTPGKNGPFSPQRRIEIMTSDLSLLSISACGKPVEAGPEKTLLRITYEPKS
                     R"
BASE COUNT         2413 a         5042 c         4175 g         2642 t
ORIGIN      
        1 actcatcggc ctttttgccc gattcggtca cctgcacgac ttcctcgatc cagcccttca
       61 tcgcgtcgcg gtccgtcttc ccgatcgtca gccccgcgat caggttctcg cacatcgtca
      121 ggacgtcttt ctgcactgtc gcatagcgct gcgagatcag cgttgccccg tagccgttgt
      181 tccgccccag ccggctgagc cggtccactg ccgccagaga cctcttttgc tcacccatcg
      241 gcctctgcgg tacgaactcc ggcgcttcct cgagaaaaat gtggtgcgga accgccggcg
      301 tcagttccat caaccggtcg caaaagtcgg ccacgaattt ccgccaggtg ttcttcgact
      361 ccagtttcag gtcgattacg cagcacaggt tttcctgaag cacggcctcg gcgatccgcg
      421 gtccctcgtc cttctcgagc ggcaggtctc cgtgcggtcc gccgaccacg aggaccggat
      481 agccgcccgg cgtcccgtcg ggattgcacc gcagacccca ccacacgctc gtcgggtcaa
      541 acgcgatcca cggaatcccg cactcacaca tctcttcggc gatcaccgtc gccgtcaccg
      601 tcttgcccca gccgcgtatg ccgatgatgg cgagcgtgcg ggtcacgaaa tcgagcggca
      661 gggtgaagtt ccgcccgctc tcgagcgtcc ccagctccag ttcatgcttc attggatctc
      721 cggatcaacg aatcgcaacc cgccttgagt cagccgatcc gtgtggactc gcagccaatc
      781 caccgcctcg tcgtaggcct ccgcaaaacc ttcgctgaac gtccgccaca acttccgctt
      841 cacctcgagg ggcaacgccg catagcaccg tatgcagaag gcgtgtttgc gctccttgaa
      901 tcctccgcag gcgcactgcc gcgacgccag cgcctccacc gcctttcgtt gaagtgacgg
      961 cgtcaggctc atgcgtcctc gtcctcctcg acaaactcgt cctcctcacc gtccacgccg
     1021 tccgggttgt agcagacgca gcactgcggc cggtgacaac attcctcgca accgtcgcaa
     1081 caaccctcgg ggcattcgcc ctcgagatcc agcggctcac cgcagcacca gcaggcatcc
     1141 gcgttcatcc tacctccagg tcttccggcg gctcgcagta gtccaacgcc tcctcgcgct
     1201 cctcttcctc gtcgtgctgc cgcccacaga ggtcgcagaa gagatcgccg ctcccagtga
     1261 cgtaagccgg gcgcagaaca tcatcgccgc ttttcacgtc gaagcagata aatcgcccgc
     1321 agtcctggca actcatctgc tcatcgccgt tctggtggca caggtcacac atcacgccgc
     1381 ctccttgtct atctgcgcaa tcagcgtttc gattcccgcg ccctgcgtag ttcccgccgg
     1441 atcaccggat gccgcgcgcc ttcgcgcagc cgcaggcaat cgggattcgt gcagactgtc
     1501 cggctcgcgt ccgcccaggt gcaggtcgtc tggtgaaaac tcgcgaggca gttccaccgc
     1561 acgacgatcc tgaagatcgt gcacgccgtc gtctccgtgc atccgcagaa gcggcactgc
     1621 ccggcctccg ttttccgtct cacgccgcct cctcacgcag gaggtcgcaa tacttgcacc
     1681 caccgcagat gccgttgaag tgccgtgcga cctcagtgtt cttgaaggcc cgccattcgt
     1741 gctgccagcg gtcgaagacg tgcacgactt tcccgctgca cacctcgtag agcaaccctc
     1801 cgcgttcgac gaatgaaatg ttcggccaca tccgatacgg tccgtcacac cgcgcgactt
     1861 cccggtggct ggctgcgtag gtggaataga acacgacggt cccagccaaa cagcaatagg
     1921 gttcgatcca ccagtcgctg ggattgatcg gcagcggttc caggacgtgc acgcactgtg
     1981 ggccgatctg cttccagtcc aggccctccg cccatttcgt atctggccgc catccgttct
     2041 tcgatagctc ctcaaagaag caccacggaa tccggggcga ccaattcggg ctccagacgc
     2101 ggccctcgac gccgaccgta tcgctcacgc caacccgccc tctttcatgc cgcctccttg
     2161 ccgtcgcggt cgagttgcag catcccctgg ccgaacagcc gcgccacgtc gctcttcgag
     2221 gtcagcgccc gcaagtaccg caggtgcgca tacacctcgc tccatcgcgc atggatcgcc
     2281 aggtccaggt cctccgccgt catcacgagg aaatatccgt gcggcttgcc ccgccggccg
     2341 cctatcggca ccttctcgag cagcaccagg ttctcgactg ctccggcaat cgcccgccgc
     2401 gagttctcgc accattccat gtggagttgc gcggcgatcg actcgctcgc taccggattg
     2461 cgacagcctc ggtgcatcgc gatcaccgcc aggacgtccc gctcgaacgg ccccagccgg
     2521 ctcgaccgca tcgccgcctc gagcctccgc tcgatctcct cgagccgctc cgtccagccc
     2581 agcgcgaatt gcatcgcctc cggcatcaga cccgcatctt gggatccgcc gacgcgggat
     2641 actcgttcca ctcgcggcca tcgagtaggc ggcccgcgcg cttctttccc gtgcgccaga
     2701 aatcgccatt gcgcctcact ggagatttcc gcgtcgccag ctcgtagtcg gtcaactgac
     2761 tctccggaca ccattcgccc cattgtttga agaaaaaagg gacgcctgcc gcttcgcact
     2821 gatcccgcag agaccgtacc cagtccgggt gcatcggtcg tgcgttcggc ccgctttccc
     2881 caccgaccac gacccaatgt agccgcttgc cacagcgagt atccggtatg gtgtctcggc
     2941 ctttccacgc cggaacatat tcgccgcgca acgcgtccat cacgagatcg cacttcatgc
     3001 ccagattgcc ctgcaaccgg cacatgctca cgaccccgag gagcggctcc gcgctcaccc
     3061 accgcaccgc cgccggcgtg tctagcagga ccggaatccg ttcgtccgcc gtcttctggt
     3121 cctcgaccga gacgccccac cagatgttgg gcgccgccga taagtcccct gggcaaccgc
     3181 gcatcgacgg cagactccaa gacttgcgca tctgttggag aacgtcccgc attcggttca
     3241 cccgcttggt gagcatctga tagatgtgct gtggagcctc gaccatcgcc tgaaaaatcg
     3301 aatgcagcca gtcctcgcgg agcttctcgt ggaaaagatc gctcgtcgag ttcacaaaga
     3361 tccgtcgcgg cttcttccag cgcaacggca cctcgagtgc agactcaacc aggcgaacct
     3421 cgccggtcca gcgcggccca gccggtctca cctccgccag gccctcatag ggctggcccg
     3481 gtccggagaa ccgcgctgcc tctcgttcgg cgtaacagtt ccggcagcct tctgaaactc
     3541 gcgagcagcc acgaatcgga ttccacgtcg cgtccgtcca ttcgattctg gaccggtccc
     3601 ccattactca cttcgcccct cgccgcggca tttggtcatc aggccgcggt ccaccgccaa
     3661 ctgttcccgg gcaacgtgga cctcaccgcc gaaaatggcg tggccgctcg gacggcgaca
     3721 ggcacgaccg gcggccgctt ggcactctgg gcaggcaacc gtcagagccg gatggacggt
     3781 ccaccgctcc ccgcatctgt ggcactccat ggccattaga cctcgctgag tctattgatc
     3841 agcgctgcct tcctctcgag cggtagcccc cgccaaatcc catccaccaa ctccggcgtc
     3901 gccacgatgg tgattgcgcc gttcttcgga ggccgttggt ctgacctctg gccgttgccg
     3961 ccggccgcga tcctctgcac gttcgacagg gacaggtgat gcttctctgc gatggccttg
     4021 tgtgtcatcc ctgccgcccg gtctcttcgg acttggacct tcaccgcctc tggggttggt
     4081 ggcctcattg ttgcctcctc aaattttgct ttgccctcgg caatcttctg ctgctgccgt
     4141 tgcgccgcaa catcggaagg atgcggccgt cccgcgaagc accactggca cattccttcg
     4201 ccgttcacga cccggtgtgc gacctgaacc cgcccgcgtt cttggcatgg tccacagagc
     4261 atcagcagtt cccgcaccac ggcagcaacg cctcgacggt ccgcccgccg acctcgacat
     4321 tcctcggtgt gtaccaccgc gtcggtctcc cgcagttgaa gcacttcatc gtcagcctca
     4381 gttgcttcgc gatccgccgc gggatcagga cggtcgctgg cccgggcagt ctcacgccag
     4441 ggtgcggagc tgagccgtcc tgcggggatc cccttctgcg tctcatccac aggcgcttgt
     4501 agactcgccg gaacgctggc accccccagc acccggggta ggtttccagg agcggcaccg
     4561 tggcgctcat cgccccgccc ccgccgtcgt caggttcggc cgcagcatct cgcgctcccg
     4621 caggtaggtg tcgtcgcatt cgacgcagcg cgtccgtccg tcgcggttca caaagtaaaa
     4681 atgcatcgtc cggcaatggc agcactccgc agccttgacg aacatgacct ggacggcgtc
     4741 gcccaggaat ccccaactct cgattgctcc ggccatctcc aggtccacga tccggtcgag
     4801 cagcgcggat ctctgtccgc tcaattcggc cttcctttgg gtgcatatcc gagcgtttgc
     4861 tgccagcctt cggcgtgttg cctccaggcc tcccagcgga cgatctcgtc gcggtaggct
     4921 ccgatgctca tctttccgct ctgaaactga gccgtggccc tctcgaaccg aactcgcgga
     4981 tggagtccct gcatccgcca gactcgcgcc gctgacgaga tgtacaacgc gaaccacagc
     5041 accgcccaca gtgcgaaccc gatgcagatc gccgccacga ccaatccgat cgcgccggcg
     5101 acgatcgcga gaatgagcag gaccagcgct tccgccgcct tgaccagcaa tgcccagctc
     5161 acgtctgcct ctctttcatt cgcgccatcc gcggccccag tcccgcccgg gcctcgcgca
     5221 cctccgccag ccgccgctgg atctgcggca cgcagtaccg gaagctcgtc acgatctgcc
     5281 cgcgctccgc ggcgaaggcg aatgcccgct ggatcccgat gaagatgtcg ccgagcggcg
     5341 cggccaccaa ctccgcccag cgtcgcacga tctcctcttc ggtcggctct cgcagttcgc
     5401 cgggtttcgg ccggttgtaa agttcagtcc ggctgagtcc cgccaggtgc aggttgtcca
     5461 ggaagaaatc ccggatccct gcgaaccggt cctcgaccgt gttctcctgc gcgtctctga
     5521 gttgctcgtt cacggttcga gttccaacac tcccttcctg aagtccacac ggaatgcgtg
     5581 gaattgcgac aacacatcca tcccgagcac cagctccacc ggctcgccta cgttctcgga
     5641 cactgcctgc agatcagtca cgatgaccgt caggccgctc catcgcttgc cgccgaattc
     5701 gagccgcacg gcgcggtagg ccatcgcggt cttgccgccg cccagggtcg tcaatggcga
     5761 ggcgccgagg tccttcttgc tcatgccagc ggcagcagga gttgtgatcg tgcggctgcc
     5821 tccggtgtct aggagggcca cccgctccac cccattcacc ttgaccttca gcaggatcac
     5881 tggctgctct ttgccgaggt ggaacttgat cgcctcctgg ccccgcagtg gcactgcgag
     5941 gagcgccgca accaccgtcg ctgccgtggc cacccgagtt gtcccgcccc agttcacgcc
     6001 gcctcttccg gtttctccct ctttcccact tcgacgtgcg gtgccagctc gcgcacgcgc
     6061 accgcccggg cgaacagcgc ccgcaagttc cgtggcgcat cctccggcag cctcccctgg
     6121 tccagcagac gctccgcgcc cggcgccagg atgaatgtct cgaccttccg gaagagccgc
     6181 cggaaaagtc ttccagtgcc cgaccggatg caggcgtact tgatgcgcat cgccgcgcgc
     6241 atatccacgg tgatctcctg cgcctccgaa acccggactt cgtaggccga gccacagagc
     6301 cgcttcgtct tccacgcacg cggggccgtg gacccatatt ccctcgtcaa ctcgatgagc
     6361 cgcgcccggc gcgctgaggc ctctcggctc gccacttcga gccccgcctg tgccccgagg
     6421 tacagcactg cgacctggtc aatcttccac gctgccagcg ttttgatttc agccatgtcg
     6481 ttcatccttc tttctggccg gggaaacggg agaaagttgg tagagtcgtt cgagttcggc
     6541 gacgtccctc gaggcgcgcc gcgcgcgccg gtgtgcgacc agccgccgct gttgccgcgc
     6601 catccgcagg gcgattgccg accgctcaag caactcgtcg ctgagcggca ggaaggtgaa
     6661 gcaatcgagg cactcgaatc ccattcgccc atcgggcccc ggcccgcgcc gccgcacgag
     6721 cgatccgtgc caggtgggac acagtcccac cgcgccgcgc agggccccga caatccgctt
     6781 gaggcctcgc ctcatcgaat ccaccccagc aggtcgagag cgatcatcgc caatacaatt
     6841 ccggcgatcg cgcagagcag tgccgcgccc cagctaaaca gtcggttccg ccgctcccgg
     6901 tcggcgtaga cccgcgccca gaactgttgc cgtgccaggc gccgccgccg cgcctccgcc
     6961 gcgtccacgt attcgggccg cagttcatca atgcaccgca ccttgtgcct ttgccactgt
     7021 tcgccgttca tgccacaccc ctctgggcgg accgtttctc ccttgctcgg tcgatccaga
     7081 tggacagctt gcgcggggaa tagaaaatgg cggggctggt cctggcgccg caggagcaga
     7141 gttcccggcc tgcctcgacg ggttgccggc aggatgcaca gatctgcgta taagggtcca
     7201 tctcccgcgc aaattcgaga atggtcttct tgaggtcctc gccgagccgc gccggccgtc
     7261 cgttcccgag ttcgcgggcg gcgatctcca acgcctctga gtcgctcatg ccggtcagtt
     7321 gtttccggtg ctggatgcga tcgcgccact gcgcgagaag aaatttgtcg cgctcgaacc
     7381 gctgatagag gtcgtggctc ccgatcagga gcaggccgca gccgtatgag tcgcggcggt
     7441 tctctgccag gtcgtttgcg gtcaggtcac gcagtcgccg cagcgtctct agggcatcgt
     7501 aaggtcgccg tagcaggtgc tgcgcctcat ccacgacgac gcagggcatt cgctcgcggc
     7561 tccggaattc cgcgatcagc gctcgcatca gcggttcgcg gagattcgac cggacgggca
     7621 cgccggcggc cgagacgatt tgttgcagca gcccgagcgg cgaaggattc ggcatcgcat
     7681 agatgtacaa gatgtcatcc cggccggccg tctggtgctc ggcgatcagg cacttgactg
     7741 caaaggtctt ctcttcggcc ggcgccccgt ggaccacggc gcaagcgtgt tgccgcaggc
     7801 aggcgttgac caggcggcgg atctctgcgg agtttcgggt ctcgaggaac cgcgccggcg
     7861 gtccgaatgg cggcggcgca ggatgcttcg cgacctcact ccagatccgc gccgtgagaa
     7921 gttcggcatt gcaggggtgg tacctcggat agtaaccctg gacccactgg ttaagcgtcg
     7981 aacgagcgat cccgagcagt tcggcggcgc tttgcgcggt gaggcctcgc cgcaccatcc
     8041 aacggttgac ttcccggcgt gcttcctcgt aagggggcgt ttctaacacc aggagttcgc
     8101 gcttcacggc gatcgggtaa gtgccgccct tgagtgccgc actcattcct ccacctccac
     8161 ctggtcgagt aacgcggcat cctcttcgct gacatccgcg gcgaatgtcc ggatcgccgg
     8221 cagcggactg cgctccggca gcgccagccg cgcgccgggc gcgccctttg gcgccggcag
     8281 gcttccggcc ccatccgtgc cggtgtggag ctgcgcccgc tggattcccg cacgctcgag
     8341 caatgcttcg agttccgtct gccagccgag ccgcgtcgag atagccgcga gcgcgccgag
     8401 gtattccgag taagcgcgct tcggccggcg ctgccggccc atggccgcgt tgatgtcctc
     8461 gcggctgatc tgtccgtgcg ccccctgcgc cacgagttgc tgcaggcgca actcgccgac
     8521 aaactggcgc gtctcggggt ctctggccac cgcctgcgtc aggtcgtacg gatcgcgcag
     8581 cacgcggata cgctgcccct gccgcaagtc cagtgcatag aggctctggt cggttggttc
     8641 gtaccgcagc cggtcgatct ccacgcaacc gccgggcagc accgtgcgtt cgcaggcctc
     8701 gagcagcatg tccagctcgc gcggttcgat cttcggccgt gactccgccg gccactgctc
     8761 atcgagcact tcggcgggcg tgcgtcctcc gagcgattcg agcggtgtac ggttgtactc
     8821 ctcgatccac tggactgctg cgacgatgaa gatgacgtcc atcggcagtg gtgattcggc
     8881 gcgtttgccc tgcaggtact tcctgtgcag gacctgcgac atcctggcga tctccggaca
     8941 ggcacccgga ctgtttccca ggtagctttc gtgccacagg cggtcgaaac gcttcgacca
     9001 acgggtgaac cagctctcga tgggcttcga acgcgggtgt ctgggcaggg cgtaggtaac
     9061 ttcgaggcac cggtcacgca acaggcgctg ggcgttttcg ctgaggcgga cctcctcctc
     9121 gatcgccccg gccttttgga agtctttgcc gttgtcccag tagagatttc tcgggaagcc
     9181 gtactgcagg gctgccatcc gcagcgccga gttgatcgtc gctgagttcg gcctcggcgc
     9241 gaagcagaaa cccacgagcg ttcgcgagcc ccagtcgtag acagccgtga gccacatccg
     9301 gtaggctgcg cctggtggca cccaggggaa cagcgcgttg ctgacctgca cgtcgaagac
     9361 gcggtggtct gcgacccacc agtccattgc cggcacgttc gaccgcaaga taaacggcga
     9421 gcacgtccgc tcgtaggcct ggggaccctc cttcgccagg actgccagcg gccgcggaac
     9481 tcctcggaga tactcggcta ccgtcgaata actagggggc gcgccgcgtt cgcgcaggtt
     9541 cggccactcg cgccggagtg cctcccagat catttgcacg ctcaggtctt ccttcaggct
     9601 ctgccccttg gcccgcacga agactttggc attgggatgg cggagaaaaa atcgcgaggt
     9661 gcccttgtcc cgccgcggtg gatcgctcag tcccgcccag ccgcccttcg tctcgtatcg
     9721 gcgcagccag cggaataacg tgcttgtgga aaccggcggc gcctgttgcg caccgacgta
     9781 cgctgccagg tctgcgagcg tctcgatggt tcgcccgtcg ccggcctgaa tcttcggccg
     9841 cgttccgttc gtccgccggc gaaagtcgag aagtggcgcg atcgccaggt atcgcgcgtc
     9901 cgccactgct ttctctgccg gcgtcaggtc gtttcgcctc gcctcaacca ctgcttccgg
     9961 cgccgcggca aataacggca gcgtgcgcgc tgccggcacg attgccgtcg cctggctcac
    10021 ctgctgctcg gcatacctga gttgcgccgc cggcgagagt cgcgtgagcg gatagcaccg
    10081 ctcgcgcttg cggttgccgg ttcggccctc cgacagttgc gattcccaaa cctcggcctt
    10141 tgccttctgg cgcacccttc tgtcggtcag cttctcgagt gcgcagatct ccctaattgt
    10201 caggtacact tcggacacag ttcccccgaa cgtgggcgcg gcggaggaat gcaaatggga
    10261 tcagtgcgag tttctccaac gaaataagga cgtcgggatg aggaccgact cgccaggcga
    10321 gcacgagcca gacaatcgtt gcgagagcgg caacgacaaa gtaggctgca tgggtgcggc
    10381 cttcgagccg cgccagggca cttcctgcgc cgaagaccag aaggtaccag ccaaggcctt
    10441 gccagagcag ccggtccggc gcggcggcgt gcatcgccgc gcagaaaaaa atcgccgcag
    10501 cggcgagcga gccgaatgtc gtcgcgataa gcgctcgcca ggccggcgtg ggcccggtga
    10561 gccgcaccag gccgagtccc agggcgacgg acagcgagat ggctgtcgtg ctgagaagaa
    10621 tgcaccaggc aggatgccgg agggccacag cagatgcgcg ccagagtgcc ggcgttaaca
    10681 cagctccgct cagtatcagt ccggccagat agaaattcgc ataccccgcg ccggccgggt
    10741 ccgcggtctg gcccgcaatg agtgtgccca gcgaaaagat tagccagacg cagaggaaac
    10801 cgaacaccgc aggacgcgac gctgccaggc caaccgccgc catccggcag agcaagataa
    10861 aaagcgccgc gtttggcacg acgatcggcc agtatttcat gcgcgcgcct ccggacaggg
    10921 gagccgcagg gcgcggtcgc tgaacagatg ccgctgctcg gggacgaatc gttcgaggtg
    10981 cttgcgcttt tttttcgccg gagtaaactg ccagctagag gaggtgctat atgcccctgc
    11041 cgccatggcc gacactgctc gcctggctcg cggcgatcct gacggtcgcc gttcgctaga
    11101 gttggcatcc ggccggcggt ccggtgtctg ctcccgaacc gccggcgtct ccgcgacaga
    11161 ttggcgctgc accgggggaa ccccacaatt ctttgtggtc gttgttcgtt gccgcatcac
    11221 cgtggtccag cttttgcctg gttgctgact tgcccggacc agaacagcgg ccgtagcctg
    11281 cctgcgcgca cttctttccg gaagatcgag atgatgccgt ccaccaggtc gagcgggccc
    11341 cacggacggc cgacctcgcg caagatcccc ccgagaaatt cgcggttctc gggcgagaga
    11401 aattcgagcc gcagcacctg ccggacgcac cacgcgcaga acggccccgc cttgcgttcg
    11461 agttcccgcg cgcggtcctc cgcctgcgcc gccagctccg gctcgagccg cactgtgatc
    11521 ggcttccgca aatccaggct catcatcggc agatggagga agtcgctcat gccgccttct
    11581 ttgagctttc gcgctcgatt cgctcgatct cagcgagcag cgcagcgatg acgcggcgcg
    11641 aagtgcgctc gccgcgtgca acgcgtgtca cctgggtgtg gctcagtccc agttgacgcg
    11701 ccacccgagc gatcaggccg cggtagagca ggacctttct gataggctgc ggaatctggg
    11761 ccgtggtaga ttgtagacgc gcacgctttg cgctcatggg cgcgacagta gcgctactgt
    11821 agcgccctgt caaggggaaa atgtgccgaa gttttccaca gggcagatcg tccgcaagat
    11881 ccgcggccgc ctcagccagc agaagtttgc tgacaggctc ggcgtgtcca tcggggcgct
    11941 acggaagtgg gaatccaaca aggcgcggcc ctgccccgaa aacctggacc acctagccca
    12001 gatcgccccg ccggaactcg ccgcgcacct cggcatccga gacacacccg ggtcgccggc
    12061 gcggaccgac ctcgccaaac aggtcgatgc cctcgaggcg cgtatccggg ctgtcgagga
    12121 agtcctccat ctccccgagc cgcgagggag ggctgagacc ctcgccgttg aaggccgggc
    12181 gtcgcccaag agaaaatcag ggacgtctcc ggctggtggt gtcactaacc acttgacagg
    12241 aacgtgacga tgcagtaaaa aagagtcacc gtcgcccggc gtgtgtcctc gcacgttctc
    12301 cgcatgtcca ctggggttgg gagttggaaa gaaccgtcgt cgcttttgtt tctccgaaag
    12361 atcgcgcccg acgaagcatc tcgcaggctg aactggaggc cataatggcc ctgcaaggag
    12421 aggtcgccca agccaaccgg acactcgccc acgcccggaa ggccatcgtc aagagattgc
    12481 ttgatggcac cccagtcgaa cctggccgga ggtcggtcgt cctcgcaccg cgacgttcgt
    12541 cagacccgat gcccctcgat gacttgttcg agttgatcgt ggaatgaaaa gccgcgcctg
    12601 tggaggttca gcatgaaacc aactcagatt ctcctcacgg tcctcgctct cgccgtgtca
    12661 acggtcgtcg tcacacccct cgccgcacag tccacaaaag ttgtctcgcg caccgcgagt
    12721 tctgccgtct tccgccagga caaaaccgct gtctggaccg cgctcatgga ggtcctcgcc
    12781 gacctgaaca ttcatccgga gcgaatcgag cgcaacaacg gcttggtctt cttccgcacc
    12841 gaagcaacct gggctgattc atggggcggc gtcaacacgg cggtccgcac catgaccacc
    12901 aagattgtca gcgggaacag tagctggcag gccttcatcg cgtccggaaa catcttttgc
    12961 aaggaactcg aggagaaccg caccgaggtc cgcgcccgct tccagttcgc tgcttacaac
    13021 ggctgggccg gatggctcgg cggcgccggc gccggctggc aacaactcga atcgaatggc
    13081 tggctcgaga acgaaatcct cgctcgcatg gagaaagcgc tcccggagcc gatgcgtctg
    13141 gccgcccgcg atgccgaggc tattgccggg gcgcaaaccg tcctgcggct tctccgcaag
    13201 gtggatgcct ccttcaagat cgacgcgacc ggcgattccc tgaccgcgct cctcgtcgaa
    13261 accgtcgccg ccgccgacga actccgcgcc ggctacaagg gccgcctccc tggcgcattc
    13321 ttcgatttgc tgagcaagtc cctcgatgcg ttcgccgcag cgcggaaacc ggatgcggcc
    13381 gtcgctgagc acctcgccca ggcccgcatt gccatcgaca acgccgcaga tcaactcgcc
    13441 gctctcattg aaccgccatc cccgcctgct gcgccgtctc tcgacttgcc cgcaggccca
    13501 ccgccggcct tctacgagga cagattgaca gttcctgccg aagcatgccc ggagggtttc
    13561 cggatcgcga tcgcgccgcg cgggatgcag gtccccatca ccagcatcgc catggatggc
    13621 ggcggcttcg actacgtcac gcccggcaaa aacggtccgt tctcacccca acggcggatc
    13681 gagatcatga cgagcgactt gtcgcttctc tcgatttccg cgtgcgggaa gccggtcgaa
    13741 gcgggccctg agaagacgct tcttcgcatc acctacgagc ccaagtcacg ctgagttccg
    13801 atgtccggat cgggctcggt tggaacctac atttttttgc gccaaaattg tgcaagagag
    13861 gcctgtttcc agttgatcgg catttccgtt ccatttccgt tcccagtggt gaccgaattt
    13921 gtcatttccg ttcccgtagt cccgtgttgt gagcaagtta ctgacttggc agtcgcggca
    13981 gttgcacatt tccgttccaa gctcgtaccg accatgacaa agttctttcc tttcagtgag
    14041 gcgcaaatta tgtaaaccac tttactgcca ccgagcgccc gaatcgctcc gcgcgccgaa
    14101 acaacaacaa cccctttccc ttccaccacc taagctcacc ccgaccctcc ttcccccact
    14161 ctcaccaaac ggcggaggac tcagggcggg cggtagaacg gaaaagtggc tggcgcggaa
    14221 acgacccaag gccccaccct tcgctgccct tggggcaggc gcgaaggatg gg
//
LOCUS       DBNGWZ010000108         1507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_514343, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000108 DBNGWZ010000000
VERSION     DBNGWZ010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_514343"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="AC16CX_03325"
     CDS             complement(<1..1010)
                     /locus_tag="AC16CX_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR3011575.1"
                     /translation="MPANLSPEYKAAEASFRKARDPRERLDWLREMLRTIPKHKGTDH
                     LQADIKSRIKELSEELEAPKRGGAARSGPPLVIRPQGAAQLALIGPPNSGKSALHVRL
                     TGSHAQVAPYPFTTQFPEPGMMLHEDVHFELVDLPAVSPEHPVPWLAGTLQTADACLL
                     VVDLGEPACVEQVQALHAVLRSKRVTLTDRWAGETPTPASADADGDPFAIKLPALMLA
                     NKADLVGDEAAEVEVFRELTGFKYPALAVSAETGQGLGEVGAWLFGHLGIVRVYTKAP
                     GKPAEKDRPFTLRRGQTVEDAARLVHKDLAHSLKYARVWSKTGVPGQHVGREHTLADG
                     DIV"
     gene            complement(1070..>1507)
                     /locus_tag="AC16CX_03330"
     CDS             complement(1070..>1507)
                     /locus_tag="AC16CX_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3011576.1"
                     /translation="TPGHASNHLCYLLEGERWLFTGDHIMQGSTVVISPPDGDMVAYL
                     ESLERLLALDLAALAPGHGHLIEKPHDEVRRLIAHRLKREHKVLDAFSRNNPATLDEL
                     LPLVYDDVSQRLHPVARRSLHAHLIKLSREGRVAEENERWRMI"
BASE COUNT          230 a          507 c          505 g          265 t
ORIGIN      
        1 acgatgtccc cgtcggcgag tgtgtgctcg cgcccgacgt gctgccccgg cactccggtc
       61 ttgctccaca cgcgcgcata cttgagcgag tgcgcgaggt ccttgtgcac cagccgggcg
      121 gcgtcctcca ccgtctggcc gcggcgcaag gtgaacggcc ggtccttctc ggccggcttg
      181 ccgggcgctt tggtgtagac gcgaacgatg ccgaggtggc cgaagagcca ggcgccgact
      241 tccccgagcc cctgccccgt ctccgccgat accgcgagcg cggggtattt gaaccccgtc
      301 agctcgcgga atacttccac ttccgcagct tcgtcgccga cgaggtccgc cttgttggcg
      361 agcatcagtg caggcagttt gatggcaaac gggtcgccgt cggcatccgc tgacgcgggg
      421 gtcggagttt cgccggccca acggtccgtc agtgtgaccc gttttgagcg caggaccgcg
      481 tgcagcgcct ggacctgctc gacgcacgcc ggctcgccaa ggtcgaccac gagcaggcaa
      541 gcgtccgcgg tctgcaacgt gccagcaagc cacggcaccg gatgctcggg cgacacggcg
      601 ggcaggtcca cgagctcgaa gtggacatcc tcgtgcagca tcatgcccgg ctccggaaac
      661 tgggtcgtga acgggtacgg cgccacctgc gcatgcgaac cggtcagacg cacatgcagc
      721 gcggatttcc cggagttggg cggaccgatc agcgcaagct gagcagcacc ctggggcctg
      781 atgacgagcg gcggcccgct gcgcgcggcg ccgccccttt tcggcgcctc gagttcctcg
      841 gacagctcct tgatgcggct cttgatgtcc gcctgcaggt gatcggtccc tttgtgcttg
      901 gggatggtgc gcagcatctc gcgcagccaa tcgaggcgtt cgcgtgggtc gcgcgccttg
      961 cggaagctcg cttccgcggc cttgtactcg ggcgagaggt tagccggcat ctaaaatcag
     1021 tgactcgtga ctggtgattc ggcggacacc cctgccctgc cgggcgccgt catatcatgc
     1081 gccagcgctc attctcctcc gcgaccctgc cctcgcgcga gagcttgatg agatgtgcat
     1141 gcagcgatcg ccgcgcaacc gggtgaaggc gttgcgaaac atcgtcgtac accagtggca
     1201 gcagctcgtc cagcgtcgcg ggattattcc ggctgaacgc gtcgagcacc ttgtgctcgc
     1261 gcttcaagcg gtgcgcgatg agccgccgca cttcgtcgtg cggcttctcg atgaggtggc
     1321 cgtgcccggg tgcgagcgcg gcgagatcga gcgcaagcaa tcgctccagt gactcgagat
     1381 acgcgaccat gtcgccgtcc gggggggaaa tcaccaccgt cgagccctgc atgatgtgat
     1441 cgcccgtgaa cagccaccgc tccccctcca gcaggtagca caggtggttc gaggcgtgtc
     1501 ccggggt
//
LOCUS       DBNGWZ010000109         2382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2277670, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000109 DBNGWZ010000000
VERSION     DBNGWZ010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2277670"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16CX_03335"
     CDS             <1..308
                     /locus_tag="AC16CX_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011577.1"
                     /translation="PLSVYLRGVGEGAQRAENWQTSSGSFQFTYLNAIILGESATTAT
                     HHEMLLKRDGFNAGIIYEVMMGSRSPYVRLEELLERGEDYDRVRVTWLKGAPKSGDS"
     gene            complement(389..>2382)
                     /locus_tag="AC16CX_03340"
     CDS             complement(389..>2382)
                     /locus_tag="AC16CX_03340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3011578.1"
                     /translation="VTILSPGAEMGQGSMTSLPLILAEEMDADWSKVAIEWAPADAGI
                     YGYTMGKNRMMAIVGSRAVMLYFNQLRTAGAQVRKVLIANAAEKWGVDPASLRTEPGY
                     VVDPASSRRMSYGEIAQFGAVPATLPTVDKSELKDKSQFRLIGKSQPRRDFPAKVNGT
                     AQYAMDVRLPGMVYATARHSPVHGGEPESWNEAQVGAMRGVLGIVRLPDGVAVIADSL
                     PHAMAARKALEVKWKQGARAEGFNSEQALMETYAKVHANPAAKAQTLDSKGDAKAAFA
                     SAAKVFKSEYRSDYGYHAQMEPLNAVARFNEAGDRVEVWEGSQAPDLSRAAVARALGF
                     ALEQVTYHQCYMGGGFGRRSLGDYAAEAARIAREARRPVKLVWTREEDIGHGMFRPQS
                     FQCLEAALDAEGKVAGWQHCVVGDGGVLLSTGIKIPYYQVPNQHIEMRGVSHGIRLKH
                     WRAVGHVFNVFAIESFVDEMAADQGVDPVDFRFQRMSISPRARVVFEKAVQMSDWKAQ
                     RPAGRALGISISERSGSLGAGVAEISLDRATGKIRVHKAWVAIDGGLVVQPEAARANI
                     ESGIIYGLSSVLHERVTVKGGVVEQSNFHDYHVLRMSDMPEEMRVEFVDRDAAPSGLG
                     EIGNPWVAAAVANAFYRLTGKRLRHMPFTPARVLETLKA"
BASE COUNT          381 a          835 c          747 g          419 t
ORIGIN      
        1 agccattgtc ggtttacctg cggggcgtcg gcgagggcgc gcagcgcgcc gagaactggc
       61 agacctcttc cggctcgttc cagttcactt acctgaacgc gatcatcctc ggcgagagcg
      121 cgactactgc cacgcatcac gagatgctgc tgaaacgcga tgggttcaat gccggcatca
      181 tctacgaggt catgatgggc agccggtcgc cgtacgtgcg gctcgaagag ctgctcgagc
      241 gtggagagga ctacgaccgc gtgcgggtga cgtggctcaa gggcgccccc aagagcggtg
      301 actcgtgact cgcggctcgt gactcgtgac gcaagaaaaa ggccgcgatc accgcggcct
      361 tttccccgtt gcctcctgga agtaaagcct acgctttgag cgtctcgagc acgcgcgccg
      421 gagtgaacgg catgtggcgc agccgcttgc cggtgagccg gtagaaggcg ttcgccaccg
      481 ccgccgcgac ccacgggttg ccgatctcgc cgaggccgct cggcgcggcg tcgcggtcca
      541 cgaactccac gcgcatttct tccggcatgt cggacatgcg cagcacgtgg tagtcgtgga
      601 agttgctctg ctcgaccacc ccgcccttca ccgtcacgcg ctcgtgcagc acgctcgaca
      661 gcccgtagat gatgccgctc tcgatgttgg cccgcgctgc ttctggctgg acgacaagac
      721 caccgtcaat tgcaacccac gccttgtgca cgcggatctt gccggtcgcg cggtcgagcg
      781 agatctcagc cacccccgcg ccgagcgagc ccgagcgctc gctgatcgag atgccgagcg
      841 cccgtccggc gggccgttgg gccttccagt cggacatctg caccgccttc tcgaacacca
      901 cgcgcgcgcg cggcgagatg gacatgcgct ggaagcggaa atccaccggg tccacgccct
      961 ggtccgccgc catctcgtcc acgaacgact cgatcgcgaa cacgttgaag acgtggccga
     1021 ccgcccgcca atgcttcagg cgtatgccgt gggaaacacc gcgcatctcg atatgctggt
     1081 tgggaacctg gtagtaagga atcttgatgc cggtggagag cagcacgccc ccgtcaccga
     1141 ccacgcagtg ctgccagccg gcaaccttgc cctcggcgtc cagcgcggct tccaggcact
     1201 gaaacgactg cggccggaac attccgtggc cgatgtcctc ctcgcgcgtc cacacgagct
     1261 tcaccgggcg gcgtgcctcg cgcgcgatcc gcgccgcctc cgccgcgtag tcgcccaacg
     1321 agcggcgccc gaagccgccg cccatgtagc actgatggta ggtgacctgc tcgagcgcga
     1381 acccgagcgc cctcgccacc gccgcgcgcg aaaggtcggg agcctggctg ccctcccaga
     1441 cttccacgcg gtcgccagcc tcgttgaacc gcgccacggc gttcagcggc tccatctgcg
     1501 cgtggtagcc gtagtcactc cgatattccg acttgaagac cttggccgcg gacgcaaaag
     1561 cggccttggc gtcccccttc gaatcgaggg tttgcgcttt cgccgccgga ttcgcatgaa
     1621 ccttggcata ggtttccatc agggcctgct ccgagttgaa accctcggcc ctggcgcctt
     1681 gtttccattt gacctcgagc gccttgcgtg cagccattgc atgcggcagg ctgtcggcga
     1741 ttacggccac gccgtctggc agccggacga tgccgagcac gccgcgcatc gctccgacct
     1801 gggcttcgtt ccagctctcg ggctcgccgc cgtgcaccgg agaatgcctc gcggtcgcat
     1861 agaccatgcc cggcaggcgg acgtccatgg catattgcgc ggtgccgttc accttggccg
     1921 gaaaatcgcg gcgcggctgg gacttgccga tgagccggaa ctggctcttg tccttgagct
     1981 cgcttttgtc cacggtaggc agggtggcgg ggaccgcgcc gaactgggcg atctccccgt
     2041 agctcatgcg ccggctggac gccggatcga cgacgtatcc gggttcggtc ctgagcgacg
     2101 ccgggtccac gccccatttc tcggcggcgt tcgcgatcag caccttgcgc acctgtgcgc
     2161 cggctgtccg caattgattg aaatacagca tgaccgcacg gctgcccacg atcgccatca
     2221 tgcggttctt gcccatcgtg tacccgtaga ttccggcgtc ggccggcgcc cactcgatcg
     2281 ccaccttcga ccagtcggca tccatttcct cggcgaggat gagaggcagc gaggtcatcg
     2341 agccctgccc catctcggcg cccgggctga ggatcgtcac ga
//
LOCUS       DBNGWZ010000110         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3285157, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000110 DBNGWZ010000000
VERSION     DBNGWZ010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3285157"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16CX_03345"
     CDS             <1..672
                     /locus_tag="AC16CX_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011579.1"
                     /translation="HGVIGALMALHHRNLNGGRGQYVDVALYEAVFNMMESVLPEYGM
                     FGFVRERSGASLPGIVPSNTYVTRDHKYVVIGANADSIFKRMMSAIGRPDLANDPALA
                     HNDGRVARTGEIEKVIGDWVAAHDLDHVLEVLEKADVPSGKIYDIADIARDPHYAARA
                     MIQQFTLKDGKTLKLPGIVPKLSETPGDTKWVGPELGEHTAEVLGALGYSAEQQRELK
                     KRGVI"
     gene            808..1728
                     /locus_tag="AC16CX_03350"
     CDS             808..1728
                     /locus_tag="AC16CX_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011580.1"
                     /translation="MLLALVVAVPAAGAYPEKNIQYIIPFVPGGESDVMARWQQTVFR
                     KKYKHEMVVVNKPGAGGGLAWSMLNSLSNDGYTIMGCNLPHIVLQPLEGQVQYKTADI
                     TPVYWFHYTADAILVAADSPYKTFGDLVKAGKAKPGALSFGGSATFSANHLAHERFNL
                     YTGVKSTYVPFKGTGELMTAVLGKHITGGMSYVPLALQQRARIRMLAVALDKRHPAFP
                     DVPTFKELGYKWVDGAYRGVAMPKSSPPEARKRVSDLMAELNRDPDILKWMADGGFEV
                     VDVPHDKNAAFMKERTKEYMEIAKLMGLLK"
     gene            1751..2200
                     /locus_tag="AC16CX_03355"
     CDS             1751..2200
                     /locus_tag="AC16CX_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3011581.1"
                     /translation="MIIRDIIKMKGSNIFSIAPAGAVSEALSVMVKNDIGSLVVMEGG
                     KLAGMLTFREVLKALEANRGSLAGLQVGDVMIKNPACGDPEDSLESLREIMNKDHIRY
                     LPVKEGDKLLGVISFHDVANAVIKETAFHNRLLKSYIKNWPEQESAK"
     gene            2432..>3305
                     /locus_tag="AC16CX_03360"
     CDS             2432..>3305
                     /locus_tag="AC16CX_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3011582.1"
                     /translation="MYDFANSGYTTVVITAVFNAYFVAEVAGRAPWATFAWTTALAVS
                     YALIVLTAPVIGAYADAHAAKKRLLLFTTMGCVVFTAGLALVGRGDLALGIALVILSN
                     FFFGTGENLVAAFLPEIAKGEALGKVSGWGWSLGYLGGLVTLGACLAYVSQAQALGAS
                     AAEFVPVTMLITAGVFAAASLPTFLFLRERALPVAGTADILRTAFARVAETLGHARRY
                     ADLWRFLVCLVFYQAGIQTVIALAAVYAQEAMGFTTRDTLFLILVVNVTAAAGAFLFG
                     QVQDRFGHVRTLAVT"
BASE COUNT          616 a          989 c         1125 g          575 t
ORIGIN      
        1 cacggcgtga tcggcgcgct gatggcgctg caccatcgca acctgaacgg ggggcgcggg
       61 caatacgtgg atgtcgcgct ctacgaggcg gtgttcaaca tgatggaaag cgtgctgccg
      121 gagtacggca tgttcgggtt cgtgcgcgag cggagcgggg cgtcgctgcc cggcatcgtg
      181 ccgtccaaca cttacgtcac gcgcgaccac aagtacgtgg tgatcggcgc gaacgccgac
      241 tccatattca agcgcatgat gagcgcgatc ggccggcccg acctggccaa cgaccccgcg
      301 ctcgcgcata acgacgggcg cgtggcgcgc accggggaga tcgagaaggt gatcggcgac
      361 tgggtcgcag cgcacgacct tgaccacgtg ctcgaagtgc tggagaaggc cgacgtgccg
      421 agcggcaaga tctacgacat cgccgacatc gcgcgcgatc cgcattacgc ggcgcgcgcg
      481 atgatccagc agttcacgct caaggacggc aagacgctca aactccccgg catcgtgccg
      541 aagctgtcgg agacgcccgg cgacacgaaa tgggtgggac cggagctcgg cgagcacacc
      601 gccgaagttt tgggcgcgct gggctatagt gccgagcagc agcgggagct gaagaagcgc
      661 ggcgtgatct gaagaggcgt gacggctggc ggctgacgaa tgacgattga cgagtgacgt
      721 ggaaccctcc acattccggt ttcgctggta tcatgggtcg cgcagaacca atataggggg
      781 cgaatgaaag ctggtcgatt tttgacgatg ctgctggctc tggttgtcgc cgtgcccgcg
      841 gcgggggctt atcccgagaa gaacatccag tacatcatcc cgttcgtgcc gggcggagag
      901 tccgacgtga tggcgcgctg gcagcagacg gtgttccgca agaagtacaa gcacgagatg
      961 gttgtggtca acaagccggg cgcgggcggc ggactggcgt ggtccatgct gaacagcctg
     1021 tcgaacgacg gctacacgat catgggctgc aatctgccgc atatcgtgct gcagccgctc
     1081 gagggccagg tccagtacaa gaccgccgac atcaccccgg tctactggtt tcactacacg
     1141 gcggacgcga tcctcgtggc cgcggacagc ccgtacaaga cgtttggcga cctggtcaag
     1201 gctgggaagg cgaagccggg agcactcagc ttcggcggct cggcgacgtt ctcggccaac
     1261 catctcgcac acgagaggtt caacctgtac acgggcgtga agagcaccta cgtgccgttc
     1321 aagggcaccg gcgagctcat gacggcagtg ctcggcaagc acatcacggg cggcatgtcg
     1381 tacgttccgc tggcgctgca gcagagggcc aggatccgga tgctcgcggt cgcgctcgac
     1441 aagcgtcatc cggcctttcc cgacgtgccc accttcaagg agctgggcta caagtgggtg
     1501 gacggtgcgt accgcggcgt ggcgatgccc aagtcctcgc cacccgaggc gcgtaaacgc
     1561 gtgtccgacc tgatggccga gctgaaccgg gacccggata ttctcaaatg gatggccgac
     1621 ggcggcttcg aggtcgtgga cgttccccac gacaagaacg cggccttcat gaaggagcgc
     1681 accaaggagt acatggagat cgccaagctc atggggctgc tgaagtagcc ggactgcaga
     1741 ggaggacagc atgatcatcc gcgacatcat caagatgaaa ggcagcaaca tcttcagcat
     1801 cgcaccggcc ggcgccgtgt ccgaggcgct gagcgtgatg gtgaagaacg acatcggctc
     1861 gctggtggtg atggagggcg ggaagctcgc cggcatgctc accttccggg aggtgctgaa
     1921 ggcgctcgaa gcgaaccggg gcagcctggc gggcctccag gtcggcgacg tcatgatcaa
     1981 gaacccggcc tgcggcgacc cggaagactc gctcgagagc ctgcgcgaga tcatgaacaa
     2041 ggaccacatc cgctacctgc cggtgaagga gggcgacaag ctgctcggcg tgatttcctt
     2101 tcacgacgtc gccaacgccg tgatcaagga aaccgccttc cacaaccgcc tgctcaagag
     2161 ctacatcaag aactggcccg aacaagaatc cgctaagtaa ttgcggtcgc cgcgtccagt
     2221 ggttgggaat ccttcccgcg agagcagacg ttctcagccg ggtccgcgga ttctttggga
     2281 aaaatctcat atgactcgtg ccggtattga cgattgccgt cgttcacgag atggccggta
     2341 gatacgtgcg cggaacgata ctttgaatga ttctccgtta agaaaaaacc tcgcgccggg
     2401 ggtacaaggg cgcgaggttt tttcttgggc gatgtacgac ttcgccaact ccggctatac
     2461 cacggtcgtc atcaccgcgg tgttcaacgc ctacttcgtg gcggaagtgg cgggaagagc
     2521 gccgtgggcg acgttcgcat ggacaacggc gctggcggtt tcgtacgccc tgatcgtgct
     2581 caccgcgccg gtgatcggcg cctacgccga cgcgcacgcg gccaagaagc gcctgctgct
     2641 cttcaccaca atgggctgcg tcgtcttcac ggcgggactc gccctggtcg ggcgcggcga
     2701 tctcgccctc gggatcgcgc tggtgatcct ctccaatttc ttcttcggca ccggcgagaa
     2761 cctggttgcg gcctttctgc cggagatcgc gaaaggggaa gcgctgggca aggtgtcggg
     2821 ctggggctgg agcctcgggt atctgggcgg cctggtcacg ctgggcgcct gtcttgctta
     2881 cgtgagccag gcgcaggctc tgggcgcgag cgccgccgag ttcgtgccgg tcaccatgct
     2941 gataacggca ggcgtcttcg ctgccgcgag cctgccgacg tttctgtttc tgagggagcg
     3001 ggcgctgccc gtcgccggaa cggctgacat tctgcgaacc gcattcgcgc gggttgcgga
     3061 aacgctgggc cacgcgcggc gctacgccga cctctggcgt ttcctcgtgt gcctggtgtt
     3121 ctatcaggcg ggcatccaga ccgtgattgc gctggcggcg gtgtacgcgc aggaggcgat
     3181 gggctttacc acgcgcgaca cgctgtttct gattctggtg gtcaacgtga cggcggcggc
     3241 gggcgcgttc ctgttcgggc aggtgcagga ccggttcgga catgtgcgca cgctcgcggt
     3301 gacac
//
LOCUS       DBNGWZ010000111         2627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2781798, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000111 DBNGWZ010000000
VERSION     DBNGWZ010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2781798"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="AC16CX_03365"
     CDS             complement(<1..1590)
                     /locus_tag="AC16CX_03365"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3011583.1"
                     /translation="MTAPEKAARVSQPRDYKLIVEKDVKIPMRDGTLLYADVFRPDGG
                     AERFPAIINISVYQKDKMWIPPADLEEKANPYMNWETVNPLWWCPRGYACVRVDSRGT
                     GKSPGKSDPSSYQESLDFYDAVEWIAKRPWCSGNIGTLGISYHASSQWRVANLQPPSL
                     KCIMPWEGRADQYRDQAYHGGIFSLGFIGNWWLTHTAHHLLGRPRSYNPDAFQNDMLW
                     NMMRNDLDSEWWRQCSARWERINVPLYSVGNWGGFALHLRGNTEGYVNAASKVKKLRI
                     HTGTHFHPFHSEEGRMDQLRWFDYWLKGVDTGILDEPPVKLEIRTGGSRKPYPLRFEN
                     EWPLARTRWSKVYLRIDREQGHEETAVEGGLVCEPPAVEKSVSYPASGVTAAGVASGS
                     SLSTTHGGGGRLGVSFQSDPMPEDSEMTGPLMLNLWVSSTSEDMDIFVTLRNIGPDGK
                     DVWEVGQHGQPVPLTKGWLRASHRKLDPAKSLPYRPYHPHNERLWLKPGKPVECQIEI
                     WPTCIVLGKGHRLRVDIQPRDG"
     gene            complement(1633..>2627)
                     /locus_tag="AC16CX_03370"
     CDS             complement(1633..>2627)
                     /locus_tag="AC16CX_03370"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3011584.1"
                     /translation="MNYRKSEAKEASRAQFRGVWAAIPTPFTPDFQVDEAGLRANMRH
                     LTRNLRIEGVFCTGVMGEFWSLTKEERKRIVEIVVEEARRGGCKVIAHTAHHSAHETV
                     ELTRHAQDVGADFVILMNQYYPPASEQTMYEWFRFVADRVDIGLWMFDAEYSGWGMSP
                     ELTARIADIPNVCGIKIPRPLDHYAKVQKLVGGKIVMSEPTEGQWLTMMRDYGQKVFQ
                     SSPAPYLMQTASWLPMREYTELGLSGRFEEAEKIAKPMQPLREVAAKWLRANWVQRKL
                     IPIAFIKAWCEMMGMAGGPVRPGLPQITEKERQELRSDLERTGILARVPAAKAA"
BASE COUNT          409 a          873 c          839 g          506 t
ORIGIN      
        1 accgtcgcgc ggctggatgt ccacgcgcag ccggtgcccc ttgccgagca cgatgcaggt
       61 cggccagatc tcgatctggc actcgaccgg cttgccgggt ttgagccaga gccgctcgtt
      121 gtgcgggtgg tacggccggt acggaagcga tttcgcggga tcgagcttgc gatgcgaagc
      181 gcgcagccag ccctttgtga gcggcaccgg ctggccgtgc tgcccgactt cccacacgtc
      241 cttgccgtcc gggccgatgt tgcgcagcgt gacgaagatg tccatgtcct cggaagtgct
      301 cgacacccac aggttcagca tcagcgggcc ggtcatctcg ctatcctcgg gcatgggatc
      361 gctctggaac gagacgccca gccgtccacc accgccatgc gtggtcgaaa gcgatgagcc
      421 cgacgctacg cccgccgcgg tgacgccgct cgccggatag ctcacgcttt tctcgactgc
      481 gggcggttcg caaaccagtc caccttccac cgccgtctcc tcgtggccct gctcgcggtc
      541 gatcctgaga tagaccttcg accaccgcgt gcgcgcgagc ggccattcgt tctcgaagcg
      601 gagcggatac ggcttgcggc tgccccccgt gcgaatctcg agcttgaccg gcggctcgtc
      661 gaggatgccg gtgtcgacgc ccttcagcca gtaatcgaac cagcgcaact ggtccatgcg
      721 cccctcttcc gagtgaaacg ggtggaaatg cgtgccggta tggatgcgca gcttcttcac
      781 cttcgaggcg gcattcacgt agccctcggt gttgccgcgc aggtgcagcg cgaacccgcc
      841 ccagttgccg acgctgtaga gcggcacgtt gattctctcc cagcgcgcgc tgcactggcg
      901 ccaccactcg gaatcgagat cgttgcgcat catgttccag agcatgtcgt tctggaacgc
      961 gtccgggttg tagctgcgcg ggcgccccag caggtggtgc gccgtatgcg tgagccacca
     1021 gttgccgatg aagccgagcg agaaaatgcc gccgtggtag gcctggtcgc ggtactggtc
     1081 ggcgcgtccc tcccacggca tgatgcactt cagcgacggc ggctgcaggt tggcgacgcg
     1141 ccactgcgag ctggcgtggt aggaaatgcc gagtgtgccg atgttgccgc tgcaccacgg
     1201 ccgcttcgcg atccactcga cggcgtcgta aaagtccagc gattcctgat acgagctggg
     1261 atccgatttg ccgggcgact tgccggtgcc gcgcgagtcc acccgcacgc aggcgtagcc
     1321 gcgcgggcac caccagaggg gattgaccgt ctcccagttc atgtacgggt tcgccttctc
     1381 ctcgaggtcc gcaggcggga tccacatctt gtccttctgg tagacgctga tgttgatgat
     1441 ggcggggaag cgttcggcgc cgccgtcggg acggaagacg tcggcgtaga gcagcgtgcc
     1501 gtcccgcatc gggatcttca cgtccttctc gacgatcagc ttgtaatccc tgggctggga
     1561 tacgcgtgcg gctttttccg gtgcggtcat tttctctcct gaaatgcgtg actcgtgact
     1621 ggtcacgcac catcaggcgg ctttggcggc gggcacccgg gcgagaatac cggtgcgctc
     1681 gagatcggag cgcagctcct gccgctcctt ctcggtgatc tgcggcagac cgggccgcac
     1741 cgggccgccg gccatgccca tcatttcgca ccacgccttg ataaaggcga ttggaatgag
     1801 cttgcgctgg acccagttcg cgcgcagcca cttcgcggcg acttcgcgca gcggctgcat
     1861 cggcttcgcg atcttctccg cctcctcgaa ccggccgctc aacccaagct cggtgtactc
     1921 gcgcatcggc agccagcttg cggtctgcat cagatacggc gccggcgagg actggaacac
     1981 cttctgcccg taatcgcgca tcatggtcag ccactgccct tcggtcggct cgctcatgac
     2041 gatcttcccg ccgaccagct tctgcacttt cgcgtagtga tcgagcggcc gcggtatctt
     2101 gatgccgcag acgttcggga tatcggcgat gcgggcggtc agctcgggcg acatacccca
     2161 ccccgaatat tccgcgtcga acatccagag gccgatatcc acgcggtcgg cgacgaacct
     2221 gaaccattcg tacatggtct gctcggacgc cggcgggtag tactggttca tgaggatgac
     2281 gaagtcggcc ccgacgtcct gggcatggcg ggtcagctcg acggtctcgt gcgccgagtg
     2341 gtgcgcggtg tgcgcgatca ccttgcagcc gccgcgccgc gcctcctcca ccacgatctc
     2401 gacgatgcgc ttccgctcct ccttcgtgag cgaccagaat tcgcccatca cgccggtgca
     2461 gaacacgcct tcgatcctga ggttcctggt gagatggcgc atgttggcgc gcagcccggc
     2521 ttcatccacc tggaagtccg gagtgaaggg cgtgggaatc gccgcccaca cgccgcggaa
     2581 ctgggcgcgg gaggcttcct tggcttccga tttgcggtag ttcattg
//
LOCUS       DBNGWZ010000112         3718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2152179, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000112 DBNGWZ010000000
VERSION     DBNGWZ010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2152179"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC16CX_03375"
     CDS             <1..659
                     /locus_tag="AC16CX_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011585.1"
                     /translation="WSPQWLDVFAKVRIRPRKTYHPGATRTFGQAPKQVALCDVREFV
                     ERFQRTLRTARPNPPAPPRRKAHAGEASEAPALAPPPSQAELAGLRQENQELRAGVAR
                     LGQQLGAARAAVEALRRVLEPEYRAMQKLFSDLDAAGANAGEIDAAVFEPWKEKVGTG
                     PARMLEALIQRNGELTRHQLSTLSGYSVGSSTFREYLGRLSRNGLIVREGERIRLRVP
                     "
     gene            694..1167
                     /locus_tag="AC16CX_03380"
     CDS             694..1167
                     /locus_tag="AC16CX_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011586.1"
                     /translation="MTGDMQRETKELAAAIHGLVIETRSMFFRAVIAVVLVVIFLVLL
                     AAVASAQTLTQVTGTIKMPNGQPVAGKIEFELSAPGRVADPALLLVQPRVPCAIIAGA
                     IAAGCMLRSNDTITDPTGTYYRVRILAADGHELMKWKKYPISGATWDIGTHTPLP"
     gene            1190..>3718
                     /locus_tag="AC16CX_03385"
     CDS             1190..>3718
                     /locus_tag="AC16CX_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011587.1"
                     /translation="MKKKILLAALALLLAVAGAEATTVTGTVKDASGAPLASGTVTFT
                     LSQEATSTEPALYLTVTVPCAITNGAIQSCTVEGNDLLSPAGTYYKVRILGAYKQEIL
                     PERNYTITQATWDLGSHQPLASAPVAAKAYQTVQDEGLPLPQRAIANFTGAGVACSDD
                     PTNTRTNCAISGGGGGGATHQANGLALAAQDPINFQDSTTIAATNPSLGNVQLGVKNN
                     SVTDAMLAPTLHLNTATFGRLNGERFADGTRNATLAAAIADCVANSCLAGDAHDSGQS
                     ITANLFAGITGAGKLSFAPVTYTVTATSTPPVNWVLQCSSGFQGNWWPPSSGQTIFSN
                     TAANTATFSLGSTSVTGCNFVGSGAGAHLNVTGEGVRVADSYIGGKQNAILIDNSGGS
                     AYNGLFERLLLTGTSDLIKANSPSGAIPNLHFRDIWFRKDDAGLRSGKGFSMTGAAAG
                     MRAFDFTSDTFSGFTDTGSGVALDLYGCGGGSISNSLFEGFTGVGSDIVFLGNCTGPV
                     FMGANVFDTYYSAINLGSVNGAFIVPQSFIGGDPAGYDYIINGGAGGARDVFIFGANA
                     ARTFGIGEREHIFFSNGTDVFHNRTGYKLAAMADPNYGVGFSWNRQVTGSVMERWFAG
                     LAADATEDWLLNNATGANFIRAKQDGSRLEFNKPVQITGGLTLAGTGGTGQFLKQKTL
                     GGAITVETIATGDLPAHAHAESDVTSLVTDLAAKEATANKNAANGYAGLNASAQIALG
                     RTPLTTRGDLLGVDLTPALVRIAIGVAGTCLTSNGTDASWGSCGGAHDLLSATHSDTT
                     AGAVARGDVITGQGVTPKWARLAKGLAGQCLQMDGSAVDVTWGSC"
BASE COUNT          725 a         1080 c         1297 g          616 t
ORIGIN      
        1 tctggtcgcc gcaatggctc gacgtctttg ccaaggtgag gatccggccg cgcaagactt
       61 atcacccggg ggcgacgcgg accttcgggc aggcgccgaa gcaggttgcc ctgtgcgatg
      121 tccgggaatt cgtcgagcgg tttcagcgga cattgaggac cgcaagaccg aacccgccgg
      181 ctccgccgag gaggaaggct catgcgggcg aggcgtcgga ggcgccggcg ctggcacctc
      241 cgcccagcca ggcggaactc gccgggctgc ggcaggagaa tcaggagttg cgcgccggcg
      301 tggcgcgact tggccagcaa ctcggtgcgg cgagggcggc cgtggaagca ctgcggcgcg
      361 tgcttgaacc ggaataccgg gcgatgcaga aactcttcag tgatctggac gcggccgggg
      421 cgaacgccgg ggaaatcgac gccgcggtct tcgaaccgtg gaaggagaaa gtgggaaccg
      481 gaccggccag gatgctggag gcgctgatcc agcgcaacgg cgagttgact cggcaccaac
      541 tctcgacgct ttccggctac agcgtgggat cgagcacctt ccgcgagtac ctcggcaggt
      601 tgagccggaa cggtctgatt gtgagggagg gcgagcgaat ccgcctgcgg gtgccgtgat
      661 ggagcagacc ggaccagcgc cgaaggagga gggatgacgg gcgatatgca gagagagacg
      721 aaggaattgg cggcggcgat ccatggattg gtgatcgaga cgcgctcgat gttcttccgc
      781 gctgtcatcg cggtggttct ggtggtgatt ttcctggtgt tgttggcggc cgtggcgagc
      841 gcacagaccc tgacgcaggt gacgggcacg atcaagatgc cgaacgggca accggtggcc
      901 ggaaagatcg agttcgagct ttcggcgccg gggcgtgtgg ccgatccggc gctgctcctg
      961 gtccagccac gagtgccctg cgcaatcatc gcgggcgcga tcgcggccgg gtgcatgctg
     1021 cgcagcaacg acacgatcac agacccgacg ggaacgtact accgggtgcg cattctggcg
     1081 gccgatgggc acgagctgat gaagtggaag aagtacccga tcagcggggc gacctgggac
     1141 atcggcacgc acaccccgct gccgtagtca gagggcgatg gaggaaggga tgaagaaaaa
     1201 gattttgttg gccgcgctgg cgttgctgct tgcagtcgca ggtgccgagg cgacgaccgt
     1261 gaccggcaca gtgaaggatg ctagcggggc gccgctggcc tcgggcacgg tcacgtttac
     1321 actttcgcag gaggccacct cgaccgagcc ggccctctac cttacggtga cggtgccctg
     1381 cgccatcacg aacggtgcga tccagagttg cacagtcgag gggaatgacc tgctctcgcc
     1441 ggccgggacg tactacaagg tccgcatcct gggcgcgtac aaacaggaaa tcctgccgga
     1501 gcggaactac acgatcaccc aggcgacgtg ggacctcggg tcgcatcagc cgcttgccag
     1561 cgcgccggtg gcagcgaagg cttaccagac cgtccaggat gagggcctgc cactgccgca
     1621 acgggccatc gcgaatttta ccggcgcagg agtggcctgc tctgacgatc cgaccaacac
     1681 ccggacgaac tgcgcgatct caggcggcgg aggcggcggc gcgacacacc aggcgaacgg
     1741 cctggcactc gcggcgcagg acccgatcaa tttccaagat tcgacgacga ttgccgccac
     1801 gaatccgagc ctcggcaatg tccagctcgg agtgaagaac aactcggtca cggacgcgat
     1861 gctcgccccg acgctgcatc tgaacacggc gaccttcggg cggctgaacg gggaaagatt
     1921 cgcggacgga acgaggaacg caacactggc tgcggcgatt gccgactgcg tggcgaatag
     1981 ttgcttggct ggcgatgccc acgacagcgg ccaatccatt acagcgaacc ttttcgccgg
     2041 gatcaccggg gccgggaaac tgtccttcgc gccggtgact tatacggtga cagccacgtc
     2101 cacgccgccg gtaaattggg tacttcagtg ttcgtcaggt ttccagggca actggtggcc
     2161 gccgagttcg ggccagacaa tcttctcgaa cacggcggca aatacggcca cattcagcct
     2221 gggctcgaca tcggtcaccg gatgcaactt cgtgggaagc ggcgcggggg cgcacctgaa
     2281 tgtgaccggc gagggcgttc gggtcgccga ttcttatatc ggcgggaagc agaacgccat
     2341 cctgatcgac aacagcgggg gttccgccta caacgggctg ttcgagaggt tgctgctcac
     2401 cgggacaagt gacctgatca aggcaaactc gccttcggga gcgatcccca atctgcactt
     2461 tcgggatatc tggttccgca aggacgatgc gggcctgcgg tcagggaagg gtttttcgat
     2521 gaccggagcg gccgcgggga tgcgggcgtt cgattttacc tcagatacct tcagtggctt
     2581 cacggacacc ggttcgggcg tcgccctgga cctctatgga tgcggtggtg gcagcatctc
     2641 gaacagtctg ttcgagggtt tcaccggcgt aggttcggac atcgtttttc tcggcaactg
     2701 caccggtcca gtcttcatgg gggcgaacgt cttcgacacg tactacagcg cgatcaacct
     2761 gggcagcgtc aacggtgcat tcatcgtgcc gcagtccttc atcggtggag acccggccgg
     2821 ctacgactac atcatcaatg gcggtgccgg cggcgcgcgg gatgtgttca ttttcggcgc
     2881 gaatgcggcg cgcacattcg ggattgggga gcgggagcac atctttttca gcaacgggac
     2941 agacgtgttc cacaaccgaa ccgggtacaa actggccgcg atggccgatc ctaactacgg
     3001 ggtgggtttc tcctggaacc ggcaggtaac tggcagcgtg atggagaggt ggttcgccgg
     3061 ccttgcggct gatgcgaccg aggactggtt gctcaacaat gctactgggg cgaacttcat
     3121 tcgagcaaaa caggacggca gccggctcga gttcaacaag ccggtgcaaa ttactggagg
     3181 actgacgttg gctgggactg gagggaccgg ccagttcttg aagcaaaaaa cgctcggcgg
     3241 cgccatcacg gtcgagacaa tcgccaccgg cgatctaccg gcacacgcgc atgcggagag
     3301 cgacgtcacc agtttggtga cggacctggc ggcgaaagaa gcgaccgcca acaagaacgc
     3361 agcgaacggc tacgccggcc tgaatgcaag cgcacaaatc gcacttgggc ggacaccgtt
     3421 gacgacgcgc ggcgatttac tgggggtgga cttgaccccg gccctggtgc ggattgcgat
     3481 tggggtggca gggacgtgcc tgacgagcaa cggtacggat gcgtcttggg gttcctgcgg
     3541 cggcgcgcac gacttgctct cggcgaccca tagcgacacg accgccggag cagtggcccg
     3601 cggcgacgtg atcaccgggc aaggcgtgac gccgaagtgg gcgcgcttgg cgaaagggct
     3661 ggctggacaa tgcctgcaga tggatggcag tgcggtggac gtcacctggg gatcatgc
//
LOCUS       DBNGWZ010000113         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1649081, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000113 DBNGWZ010000000
VERSION     DBNGWZ010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1649081"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16CX_03390"
     CDS             complement(<1..670)
                     /locus_tag="AC16CX_03390"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3011588.1"
                     /translation="MTRKAAIVLAAVLIAACALLPTDYNGAGAPRQTTIVPLKSARPL
                     VALALGSGGARGFAHVGVIKALEEAGIVPDIVTGSSSGAVVAALYASGRRAGELEEIA
                     LAVEKGDLVDFVPFGKGWVKGEALQDFVNRMVDERPIERLARPFAVVATEAKNGRMTV
                     FNRGDTGLAVRASASVPSVFVPPVINGEEYVDGGLTSPVPVKLARVMGADVVIAVDVS
                     WFAQA"
     gene            852..1835
                     /locus_tag="AC16CX_03395"
     CDS             852..1835
                     /locus_tag="AC16CX_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011589.1"
                     /translation="MNRRHLFSCPLALALLASAVPVIAQETAKDYPSRPIRLLVPNAP
                     GSSVDTLSRIVGTKLSEVIGQQVVMDNRAGAGGIIGMEIAKAANPDGYTLISATTAAS
                     TIARLLQKNPTFHPVTDYDYVVQFAETPNVLVVNPALPVKSVKELIDYAKGKQGKVNM
                     SSAGSGSQSHLSGAYFLQAAHFESLHVPYKGGGPSVASVVAGESQWTLIPAPAAMSQV
                     NAGRLRAIAHSLPKPTPLLPNIPPIAETIPGFDYSGWQGFFLPKGTPKGIAEKLRVAV
                     VKTMEQPDVKKQFAFQATEIVVRGPAEFRKVVQESMDKNAKVVKAVGLTAN"
BASE COUNT          393 a          686 c          633 g          350 t
ORIGIN      
        1 gcgcctgggc gaaccaggac acatccaccg cgatcacgac gtcggcgccc atgacgcgcg
       61 ccagcttcac cggcaccgga ctcgtcagcc cgccatccac gtactcctct ccattgatga
      121 cgggaggaac gaacacgctg ggaacgctcg ccgaagcgcg cacggccagc ccggtatcgc
      181 cgcggttgaa caccgtcatg cggccgttct tcgcctcggt ggcgaccacc gcgaagggcc
      241 tcgccagccg ctcgatcggc ctctcatcga ccatgcggtt cacgaaatcc tgcaacgcct
      301 cgcctttgac ccaccccttg ccgaacggca cgaagtccac caggtcgcct ttctcgactg
      361 ccagagcgat ctcctccagt tcgcccgcgc ggcgcccgct ggcgtaaaga gcggcgacca
      421 cagccccgga gctcgatccc gtgacgatgt caggcacgat gcccgcttct tccagcgcct
      481 tgatcacgcc gacatgggca aaaccgcggg cgccgccgct gcccagcgca agcgcgacca
      541 gcggccgcgc cgacttgagc ggaacgatgg ttgtctgccg cggcgcacct gcgccgttgt
      601 agtcggtcgg cagcagggcg cacgccgcaa tcaggacggc ggcaaggacg atggcggctt
      661 tgcgcgtcat gggagcggga ttttaattca aagcgtcgct tgagcgacag cgttttggcg
      721 gatcgagttc ccgagtcaca aaaccggaga gcggcgtctc gtcggttgct ttcatattac
      781 aaaccggaca gaattagcct ccataatacg cgttcaacac gcgcatccga taccaagaac
      841 aggaggaatc catgaatcgc cgtcatttgt tttcttgtcc gctcgcattg gcgctgctcg
      901 catccgccgt gcctgtcatc gctcaggaga ccgcgaagga ctatcccagc cggccgatac
      961 gcctgctggt gccgaacgcg ccgggcagct cggtggatac cttgagccgc atcgtcggca
     1021 ccaagctctc cgaagtgatc gggcagcaag tcgtgatgga caaccgcgcc ggggccggcg
     1081 gcatcatcgg catggaaatc gccaaggccg cgaacccgga cggctacacg ctgatcagcg
     1141 caaccacggc ggcctccacc atcgcccgtc tgctccagaa aaaccccact ttccatcccg
     1201 tgacggacta cgactacgtc gtgcagttcg cggagacgcc caatgtgctg gttgtgaatc
     1261 cggcattacc ggtgaaatcg gtgaaagagc tgatcgacta cgccaagggc aagcaaggca
     1321 aggtcaacat gtcctccgcc ggctccggct cgcaaagcca tctttccggc gcttacttcc
     1381 tgcaagccgc tcacttcgag tccctgcatg tcccgtacaa gggcggcggg ccgtcggtcg
     1441 cttccgtggt ggcgggcgag tcccagtgga cgctgatacc cgcgccggca gcgatgtcgc
     1501 aggtcaacgc tgggcggctg cgcgcgattg cccacagcct gcccaagcct acccccctgc
     1561 tgcccaatat tcccccgatc gccgaaacca taccgggctt cgattacagc gggtggcagg
     1621 gtttcttcct gcccaagggc acgccgaagg gaatcgcgga aaaattgcgt gtggcggtgg
     1681 tgaagacgat ggaacagccc gatgtgaaga aacagtttgc tttccaggcg acggagatcg
     1741 tcgtccgggg tccggcggag ttccgcaagg tggtgcagga gtcgatggac aagaacgcca
     1801 aggtggtgaa ggcggtcggt ttgacggcca actgaccccc ggctgaggac gtgattcgtg
     1861 attcgtgaat catgattcga tcggccactg atccccaggg gcggctgcgg ccgcccttgt
     1921 cgtttcgaag tgaccatgcg gcatagttca agtgaatccg gcggcgcgtt ggtgccgggc
     1981 ggcaaacgcg atctcggtca gctcaagcag gaagtccggc ggcgcgccga ccgcaacctg
     2041 ccgccgctcg gcggcatcgc ac
//
LOCUS       DBNGWZ010000114         3512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_11189, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000114 DBNGWZ010000000
VERSION     DBNGWZ010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_11189"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..852)
                     /locus_tag="AC16CX_03400"
     CDS             complement(217..852)
                     /locus_tag="AC16CX_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_function: GO:0008289 - lipid binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiJ"
                     /protein_id="HIR3011590.1"
                     /translation="MKADRRIYGLPLNPESRILSPAVAAAINHLLRGASWARDELKRF
                     AGRTARFEVAPFALSFTVLDSGEVAPAAADAASTVTIRLTPGLMLRLAAHEETAWREI
                     ETTGDTDFASAIHHVARNLRWDIEEDLSRVFGDITAHRVAESGRTLRRWSEQALENTG
                     RSFAEYWTEEQPLIAGARDLAEFNRSVDRLRDDVARLEKRLENLVNRQQRK"
     gene            complement(901..1740)
                     /locus_tag="AC16CX_03405"
     CDS             complement(901..1740)
                     /locus_tag="AC16CX_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR3011591.1"
                     /translation="MKTCLAVVIALLSLPLIIEDADARRLGGGRNLGTQRSAPAQQQA
                     APGAPAQQQQQAAPATPAQQPAGASKWLGPLAGFALGAGLAALFLNNGWAGLLAGLLL
                     IGLIVAALLFAARALRGRVEQQPFQYASAGGVEPVLTTLPGGAGANSVVATTSRWPAE
                     FDAAEFVRHARLNFVRLQEAHDKKDLSTMRDFLTPEVYREIENDIRRAGDTPQKTEVL
                     TLEAEVIDVVEEAGSYVVSVRFSGLIRETADREAEPFSEIWHLQKPISGRSGWLVAGI
                     QQA"
     gene            complement(2032..2736)
                     /locus_tag="AC16CX_03410"
     CDS             complement(2032..2736)
                     /locus_tag="AC16CX_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011592.1"
                     /translation="MKRLLSLSALLLCAGCASSLPQTPPAGIPAQVAAPDIRTGDDWK
                     YSLHDGYTKLPRGMLEYRVIAVQGDTVTVQLRHEGRDSTERYTRDWNWRERPMTNLQN
                     FRYEPAYPALPFPLEAGKSWRAYVKATDPATGRVNRVRIDGAVLGWERVKVPAGEFDA
                     LKVRRLVYAGNPDHFLSEEYITELDWYAPSVGGIVKHASRSGHYDRRIGCDDRACSQW
                     VSNDWNAVELMSHSRR"
     gene            complement(2827..>3512)
                     /gene="ubiE"
                     /locus_tag="AC16CX_03415"
     CDS             complement(2827..>3512)
                     /gene="ubiE"
                     /locus_tag="AC16CX_03415"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR3011593.1"
                     /translation="PRRVAGVFDSVASRYDLMNDLMSGGLHRLWKRFTVDASGMRPGE
                     SVLDIAGGTGDLARLFVRRVGAQGEVVLTDVNPAMLRLGRDRLLDKGVVLPVVQCDAE
                     KLPFASDRFDCVSVAFGLRNMTHKDTALAEMGRVLRPGGRLLVLEFSRVWEPLKPLYD
                     AYSFGVLPRLGQWVANDANAYRYLAESIRLHPDQESLKRLMEQAGFERVEYFNLAAGV
                     VALHRGYKL"
BASE COUNT          592 a         1169 c         1134 g          617 t
ORIGIN      
        1 ccgctcgtgg ccgaggaaaa actcgtcgag cccgtagcgg acgctgacgg agagtatttt
       61 cagcaatcga aacaagtgca tgaggcgatt gtaaccgccg ggacgccaac cacgccaaac
      121 agcgttaaat ccggacgcat gcgccggttt gagcgcggtt cgaacggacg gaatatgcgc
      181 cgttcaacac gcggttcgaa tctttcttgg gatgctctat ttccgttgtt ggcggttcac
      241 aagattttca aggcgctttt caaggcgcgc gacgtcgtcg cgcagccggt ccaccgaacg
      301 gttgaattcc gcgagatcac gcgctcccgc gatgagcggc tgctcctcgg tccagtactc
      361 ggcgaaagaa cggccggtgt tctccagggc ctgctcgctc cagcggcgca atgtccgccc
      421 ggactccgcc acgcggtggg cggtgatgtc gccgaagacg cggctcaggt cttcctcgat
      481 gtcccagcgc aggttgcgcg cgacatgatg gatggcggag gcgaaatccg tgtcgccggt
      541 ggtctcgatc tcgcgccacg ccgtctcttc gtgcgcggcg agccgcagca tgagccccgg
      601 ggtgagcctt atcgtgacgg tggaggcggc atcggccgcg gcaggggcga cctccccgct
      661 gtccagcacg gtgaacgaga gcgcgaacgg cgcgacctcg aagcgggctg ttctgccggc
      721 aaagcgcttg agctcgtcgc gcgcccagct cgcgccgcgc agcaggtggt tgatcgcggc
      781 ggctacggca ggactcagga ttcgggattc gggattcagg ggaaggccgt agatcctgcg
      841 gtcagctttc agcgttcagc tatcagcttc ttcggctgac ggctgatcgc tgattgcttg
      901 ctatgcctgc tggatgccgg cgaccagcca gcccgagcgg ccgctgatcg gcttctgcag
      961 atgccagatt tcgctgaacg gctcggcttc gcggtccgcc gtttcgcgga tcaagccgga
     1021 gaagcgcacg ctgaccacgt aggagccggc ctcctcgacg acgtcgatca cttcggcctc
     1081 aagggtcagc acctcggtct tctgcggcgt gtcacccgcc ctacgaatat cgttctcgat
     1141 ctcccgatag acctcggggg tcaggaagtc gcgcatcgtc gagaggtcct tcttgtcgtg
     1201 tgcttcctgc agccgcacga agttgagcct ggcgtgacgc acgaactccg ccgcgtcgaa
     1261 ttccgccggc cagcggctgg tcgtggctac aaccgaattc gcgcctgcgc cgccgggcag
     1321 ggtggttagt acgggctcta cgcctccggc actcgcgtat tggaacggct gttgctcgac
     1381 cctgccgcgc aacgcccgcg ccgcgaacag gagggccgca acgatcaggc cgatcaggag
     1441 caaacccgcg agcagaccgg cccagccgtt gttgaggaac agcgcggcaa gccccgcgcc
     1501 aagggcgaat ccggcaagcg ggccgagcca cttcgacgcg ccggccggct gctgcgccgg
     1561 cgtcgcaggt gcggcttgct gttgctgctg cgccggcgcc ccgggcgcgg cctgctgctg
     1621 tgcgggagcg ctgcgttgcg tgccgagatt gcgcccgccg ccgaggcgcc gcgcgtcggc
     1681 gtcctcgatg atcaagggca gcgatagcaa cgcgattacg actgctaaac aggttttcat
     1741 atttcctcct gcggtactct gggattacgc gttgaacggc gcgcagcgtc cgttcgaacg
     1801 tgtccggcct ctgacacgtt gtcggcaata tcggcggcgc tacatcggcc gcttgcgcgt
     1861 ctccggggtc gcacccgcaa gcgagcacct gctcaaccct tcgccgcgct caaaccggcg
     1921 cgtatagcgt ccggatttaa cggaaattat acgcgcagtc atgcagcgcc cggcctcctg
     1981 cgctgccgca gacagaaccg cagcgtattc aggccgttgg atcgaagcgg atcagcgcct
     2041 cgagtgactc atcagctcga ccgcgttcca gtcgttgctg acccactggc tgcacgcacg
     2101 atcgtcgcaa ccgatacgcc tgtcgtaatg gccggaacgg ctcgcatgct tcacgatccc
     2161 gcctacgctg ggcgcgtacc agtcgagctc ggtgatgtat tcctcgctca gaaagtggtc
     2221 ggggttgcct gcgtacacca gccggcgcac cttcagcgcg tcgaattcac cggccgggac
     2281 cttcacgcgc tcccacccca gcaccgcgcc gtcaatgcgc acgcggttca cgcgtccggt
     2341 tgcgggatcg gtcgccttca cgtatgcccg ccagctcttg cccgcctcca gcggaaacgg
     2401 cagcgcgggg taagcgggct cgtagcggaa gttctgcaga ttcgtcatcg gccgctcgcg
     2461 ccagttccag tcgcgggtgt agcgctcggt tgaatcgcgc ccctcgtggc gcagttgaac
     2521 cgtgacggtg tcgccctgga ccgcgatcac gcggtattcc agcatgcctc tcggcagctt
     2581 ggtgtagccg tcgtgtagcg aatacttcca gtcatcgccc gtccggatgt ccggggcggc
     2641 aacctgcgcc gggatcccgg caggcggtgt ttgcggcaag gagctggcac agccggcaca
     2701 gagcagcaat gcggaaagag acaggagtcg tttcatgggc acctccacgt ttcgaaacgg
     2761 cgcacccgcg ttccagctaa caaccggtat gacgagcgta ttccagccgg gccgcgggcc
     2821 cgtggatcag agcttgtagc cccggtgcag ggcgaccacg ccggcggcga gattgaaata
     2881 ttcgacccgc tcgaaaccag cctgttccat caggcgcttg aggctttcct ggtcggggtg
     2941 caggcggatg gattcggcga ggtagcggta ggcattggcg tcgtttgcca cccattgccc
     3001 aagccggggc agcaccccga acgagtaggc atcatagagg ggcttgagcg gctcccatac
     3061 cctcgagaac tcgagcacga gcagcctgcc cccgggcctg agcacgcgcc ccatttccgc
     3121 gagcgccgta tccttgtgcg tcatgttgcg cagcccgaag gcaacgctca cgcaatcgaa
     3181 gcgatcggac gcgaacggca gcttctcagc gtcgcattgc acgaccggca gcaccacgcc
     3241 cttgtcgagc aaacggtcgc ggccgagcct gagcatcgcg ggattgacgt ccgtgagcac
     3301 cacttcgccc tgtgccccga cgcgacgcac gaacagccgg gccagatcac cggtgccgcc
     3361 cgcgatgtcg agcacgctct cgcccgggcg catcccgctt gcatcgaccg taaagcgctt
     3421 ccacagccgg tgcagcccgc ccgacatgag gtcgttcatg aggtcatagc gcgacgcgac
     3481 cgagtcgaag acgcccgcga cgcgccgcgg ct
//
LOCUS       DBNGWZ010000115         7480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_893029, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000115 DBNGWZ010000000
VERSION     DBNGWZ010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_893029"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..280
                     /gene="cspE"
                     /locus_tag="AC16CX_03420"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             74..280
                     /gene="cspE"
                     /locus_tag="AC16CX_03420"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HIR3011594.1"
                     /translation="MATGTVKWFNDAKGYGFITPDDGSEDLFAHFSAIQMSGFKTLKE
                     GQKVSFEVTQGPKGKQASNIQAAS"
     gene            complement(484..1860)
                     /locus_tag="AC16CX_03425"
     CDS             complement(484..1860)
                     /locus_tag="AC16CX_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011595.1"
                     /translation="MNERLDLLKIAREEQSGDLFKLLDSIYKRSKIQPRGISDAIIWE
                     GGSSSGGVKPVAHAFTDMFALNGTLMALDVLGLPFLGVPMMAQFRHDTRLAPLDWFGK
                     LEYTAIKWSTAGDFLVNENGRKVVVMGKSANAPLRTKAGVYCNVRPYGTITSVRFMPG
                     LPYSQDGKKFRVDPETGNIHSEMNTPGVRMAYAVRLFLKMVHETRQIGRVATKPTQAQ
                     EDAVNAGILRWLLQGTKFELKRRYSVDAYAEHKANVDAIIALLPKDFKAGMENWGGEI
                     NEHISDTNFGILLHDMIRQRKAIVYATDLDGDRLTDLMADAPDVLRDWGQLVLDHVKA
                     EKDMKRVWNEMGFTMKSGKDMTSVMYRMQGEPIFEQTLGSADAMLLRLLAGDETVGGE
                     KQPYDPRIHFVLINEAYKAANPGNQELARWLDAQLAAFDKIYGGKRPRYIEGYNKLKD
                     AIKPWGSK"
     gene            2207..3838
                     /gene="aceB"
                     /locus_tag="AC16CX_03430"
     CDS             2207..3838
                     /gene="aceB"
                     /locus_tag="AC16CX_03430"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR3011596.1"
                     /translation="MSRALALPPGVEIRAHLTPEYSTILTPQTLVFVAKLARKFEGRR
                     RELMAARARRQSEFDAGKLPHFLPETRHIRDTEWVVTPVPRDLLDRRVEITGPTDRKM
                     VINAFNSGANVFMADFEDANTPTWQNMVEGQINLRDAVNRSIDFLSPEGRRYELNANT
                     ATLMVRPRGWHLMEEHVHIDGAPVSGAIFDFALYFFHNAKALLARHTGPYFYLPKLES
                     HLEARLWNEIFVTAQRELGMPQGTIKATVLIETVLAAFEMDEILYELREHCVGLNIGR
                     WDYIFSCIKKFRSNRDFCLADRAQVTMTSPFMRAYALLLIKTCHHRNAFAMGGMAAQI
                     PIKNDPAANQAALEKVRADKIREATDGCDGTWVAHPGLVEIAKAVFDRYMPALNQVHK
                     KRDDVKVTARQLLDFQPEKPITEAGLCNNISVGIQYIGAWLAGNGCVPVFNLMEDAAT
                     AEISRSQIWQWIRSPKGVLEDGRKVTRDLFHRLLAEELAKVKTLLGAEGWAAGRYEDA
                     ARLFEEITISDDYVEFLTLPGYERLTRTLREPVAA"
     gene            4088..4360
                     /locus_tag="AC16CX_03435"
     CDS             4088..4360
                     /locus_tag="AC16CX_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011597.1"
                     /translation="MSKLLSTLLAAVFAAVTVTPVAFAQEKKDEKKMEMKKEMKGEEK
                     KAMKAEKAEPKKMSEEKKKVAPKKKGEGKKATPKKKGEAKKAEEKK"
     gene            4548..4793
                     /locus_tag="AC16CX_03440"
     CDS             4548..4793
                     /locus_tag="AC16CX_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011598.1"
                     /translation="MKKLLAAIVAGMFALTAATPLIAQDKKKVEKKATKTEMTAEKKE
                     AKATQKKGEQKKMTKGAGKKTEGKKTAKKGADTKKEK"
     gene            4918..5364
                     /locus_tag="AC16CX_03445"
     CDS             4918..5364
                     /locus_tag="AC16CX_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3011599.1"
                     /translation="MWKPSVTVAAVAERDGRFLLVEEHTAHGILFNQPAGHLDPGESL
                     VEGVARETLEETAHIFKPTGLLGVYQYHSRADDVTYIRFAFIGEITGHDAARALDEGI
                     ICTVWLTPEEIRRETARHRSPLVMRCIDDYLAGRRYPLDVLYRHPS"
     gene            5369..6523
                     /gene="mnmA"
                     /locus_tag="AC16CX_03450"
     CDS             5369..6523
                     /gene="mnmA"
                     /locus_tag="AC16CX_03450"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3011600.1"
                     /translation="MTGKRVIVGMSGGVDSSVAALLLKQQGHEVSGLFMKNWEDDDTD
                     EYCSSREDLVDAVAVADRIGIEIDVVNFSAEYKARVFNDFLREYQAGRTPNPDVLCNA
                     EIKFKAFLDHALGLGADCIATGHYAQIREVDGLYQLLQAEDGTKDQSYFLYRLNQSQL
                     ARTLFPLGRLQKREVREIARREGLPNHDKKDSTGICFIGERPFREFLNRYLPDNPGEI
                     RTTGGRLLGRHNGLMFYTLGQRQGLGIGGSRGSDGEPWYVAAKDLPHNRLIVVQGHDH
                     PALLSDRLTAVDLNWISGTVPHCQWVYTAKTRYRQKDAPCTLVAAGVERCVIEFAEPQ
                     WAVTPGQSVVVYESQVCLGGGIIESAERARDDGEGEEKCPPGSVDMKDEG"
     gene            6840..>7480
                     /locus_tag="AC16CX_03455"
     CDS             6840..>7480
                     /locus_tag="AC16CX_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdH-redoxin"
                     /protein_id="HIR3011601.1"
                     /translation="MIDVLNDAGGREKLLKLGVRNVPVVAKGENYVFGQNLEDVAEFV
                     GLQGTGHQPLPPDQLIKKWVNVLRAAQRYIRQIPAQRIHERVIDNRDRSIRLLSHHIF
                     RIGEAFLESAINGVEYSIGHANVPPKDGTFLTGAEIAGYGDEVIKRLQQWWGSLKDPS
                     LGWKVKTYYGMQPMHVVFERSTWHSAQHARQLIHVLERFGIEPDCRLTTEDLAG"
BASE COUNT         1540 a         2307 c         2282 g         1351 t
ORIGIN      
        1 gcccgtacgc gatcttgaaa ctcaaacagc gtgcgggcgc gcctgcccgc taatttccag
       61 cagaggtagg tgtatggcaa caggtacggt gaagtggttc aacgacgcca agggttacgg
      121 gttcatcacc cccgacgacg gcagcgagga tctgttcgct cacttctcgg cgatccagat
      181 gagcggtttc aagaccctca aggaaggcca gaaggtgtcg ttcgaggtaa cgcagggccc
      241 caagggcaag caggcctcga acattcaggc cgcttcctga cgccgtccgc cggggaaccc
      301 ggcggatcct acgaataaaa agcccacgca acctgccggt cgcgtgggct ttgccatatg
      361 gggccgcggc gaggcgcggt ccttcctccg tgaccggctt gaagccggcc gcgcttactt
      421 cattctgcgc ttaagctgcg cgtcgatcgc atccatgatc gggcccccgg cgcggaaacg
      481 cgcctacttg ctgccccaag gcttgatcgc gtccttgagc ttgttgtaac cctcgatgta
      541 gcggggccgc ttgccgccgt aaatcttgtc gaaggccgcg agctgcgcgt ccagccaacg
      601 agcgagttcc tggttgcccg gatttgccgc cttgtaagcc tcgttgatca ggacgaagtg
      661 gattctcgga tcgtagggct gcttctcgcc gcccaccgtc tcgtccccgg cgagcaggcg
      721 caggagcatc gcgtcggctg aacccagcgt ctgctcgaag atcggctcgc cctgcatgcg
      781 gtacatgacg ctcgtcatgt ccttgccgct cttcatggtg aagcccatct cattccaaac
      841 tctcttcatg tccttctcgg ccttgacgtg gtcgaggacc aattgccccc agtcgcgcag
      901 cacgtcgggg gcatccgcca tcaggtcggt cagccggtcg ccgtcgaggt ccgtcgcata
      961 gacgatggcc ttgcgctgcc ggatcatgtc gtgcagcaga ataccgaagt tggtatcgga
     1021 gatgtgctcg ttgatctcgc cgccccagtt ttccatgccg gccttgaagt ccttgggcag
     1081 cagggcgatg atcgcatcga cgttcgcttt gtgctcggcg taggcgtcga cggagtagcg
     1141 tcgcttgagc tcgaatttcg tgccctgcaa cagccagcgc aggatccctg cgttgacggc
     1201 gtcctcctgg gcctgtgtcg gcttcgtggc gacgcgcccg atctgacgcg tttcatgcac
     1261 catcttcagg aacaggcgca cggcgtatgc catcctgacc cccggggtgt tcatctccga
     1321 gtggatgttg ccggtctcgg gatcgacccg gaacttcttg ccgtcctgcg agtacggcag
     1381 gccgggcatg aaacgcacgc tggtgatcgt gccgtagggc cggacgttgc agtagacccc
     1441 agccttggtg cgcagcggcg cgttggcgga cttgcccatg acgaccacct tcctgccgtt
     1501 ctcgttgacc aggaagtcgc cggccgtgga ccacttgatc gcggtgtact cgagcttgcc
     1561 gaaccagtcg agcggcgcga gcctggtgtc gtgccggaac tgcgccatca tcggcacacc
     1621 gaggaacggc aatcccagca cgtcgagcgc catcagcgtg ccgttgagcg caaacatgtc
     1681 ggtgaacgcg tgcgcgaccg gtttcacgcc gccggatgag cttccgcctt cccagatgat
     1741 cgcgtcggaa atgcctcgcg gctggatctt cgaccgcttg taaatgctgt ccagcagctt
     1801 gaacagatcg ccgctctgtt cttcccgcgc gatcttcaac aggtcgagtc gttcattcat
     1861 gaggcttctt ctccgttcca aatatgggtc aagtgctaga ggtaatccgg ctatctgtgg
     1921 aggcatccag accgaatccg ggtgcagatc gttcctgcta ctgcgtgctc ccgtccttgg
     1981 gggaagttgt ggatgaccag tgctttacga cgcagtgcat cgaaatgcga tgctgatagg
     2041 tctgaattat aaccgcgggg cgtgtcttgt gagttttgcc aattggactt gatctagagc
     2101 agtgtataat gcagtgcagc atcggttacg gtatatattt tcacaatgtg caatagaaaa
     2161 tccaagccgt aaccgttcaa ttaatgatac gaggacaggt gacaccatga gccgcgcatt
     2221 ggcattgccg ccgggcgtcg aaatccgcgc ccatttgacc cccgaatact cgacgatcct
     2281 caccccgcag acactcgttt tcgtcgcgaa gctcgcgcgc aaatttgaag gccgccgccg
     2341 cgagctgatg gcggcccgcg cgcgtcgcca gtccgaattc gacgccggca agctgcccca
     2401 cttccttccg gaaacccggc acatccgtga cacggagtgg gttgtgacgc cggtgccgcg
     2461 cgatctgctc gaccgccgcg tcgagatcac cggaccgacc gaccgcaaga tggtaatcaa
     2521 cgccttcaat tccggtgcca acgtcttcat ggccgatttc gaggacgcca acaccccgac
     2581 ctggcagaac atggtcgagg gccagatcaa cctgcgcgat gcagtcaacc gcagcatcga
     2641 tttcctcagc cccgagggca ggcgctacga gctcaacgcg aacaccgcga ctcttatggt
     2701 gcgcccgcgc ggctggcacc tcatggaaga gcacgtgcac atcgacggcg cgccggtttc
     2761 gggcgcgatc ttcgatttcg cgttgtactt ctttcacaac gcaaaggctt tgctcgcgcg
     2821 ccataccgga ccctattttt acctgccgaa gctggaaagc catctcgaag ccaggctctg
     2881 gaacgagatc ttcgtcacgg cacagcgcga gctcggcatg ccgcagggca cgatcaaggc
     2941 cacggtgctg atcgaaaccg tgctcgccgc cttcgagatg gacgagatcc tctacgagct
     3001 gcgcgagcac tgcgtcgggc tcaacatcgg gcgctgggac tacatcttca gctgcatcaa
     3061 gaaattccgt tcgaaccggg atttctgcct cgccgaccgc gcgcaggtga ccatgacctc
     3121 gcccttcatg cgggcctatg ccctgctgct catcaaaacc tgccaccacc gcaacgcttt
     3181 cgccatgggc ggcatggcgg cgcagattcc gatcaagaac gatccggctg cgaaccaagc
     3241 cgcgctggag aaggtgcgcg ccgacaagat ccgtgaagcc accgacggtt gtgacggcac
     3301 ctgggtggcg catcccggtt tggtggaaat cgcaaaggcg gtattcgacc gatacatgcc
     3361 ggcactcaat caggtgcaca agaagcgcga cgacgtcaag gtcacggcga ggcaattgct
     3421 cgatttccag ccggagaagc cgatcaccga ggccgggctg tgcaacaaca tcagcgtcgg
     3481 catccagtac attggcgcct ggctcgccgg caatggctgc gttccggtgt tcaacctgat
     3541 ggaggacgcc gccaccgccg agatctcgcg gtcccagatc tggcaatgga tccgcagtcc
     3601 caagggcgtg ctcgaggacg gacgcaaggt aacccgggat ctcttccaca ggcttctggc
     3661 ggaggaactc gccaaggtga agaccctgct cggcgcggaa ggctgggcgg cgggccgtta
     3721 cgaggacgcc gccaggctgt tcgaggaaat caccatcagc gacgattacg tcgaattcct
     3781 caccttgccc ggatacgaac ggctcacgcg cacgctgcgc gaacccgtcg ccgcctgatg
     3841 attccgcttg cagcatggct gagccctcca cggagctggg cttgttcgcc cctgcccctg
     3901 cctcaaccgc gcggaatcat tcacaacgta ttgtattcga agctatttta ttaatttccg
     3961 gcaagcactc gccggagaac ggttagggct gtcaaccggt ttgtcgcgcc gggcgttaag
     4021 agcgttgacc gaaatttcgg tcgtttgccg caaacaacca gccattcgac taccaagagg
     4081 aaatcgcatg agcaagcttc tgagcacgtt gctggccgcc gtcttcgccg cggtgaccgt
     4141 gaccccggtc gcgttcgcgc aggagaagaa ggacgaaaag aagatggaaa tgaagaagga
     4201 aatgaagggc gaagaaaaga aggcgatgaa ggccgagaag gccgaaccca agaaaatgag
     4261 cgaagaaaaa aagaaggtcg ctccgaagaa gaaaggcgaa ggcaagaaag ccacgccgaa
     4321 gaagaagggc gaagccaaga aggccgaaga gaagaagtaa tttctcccgc tatcgcgaaa
     4381 aaggcagggt cagcgccctg cttttttgtt gcccgcgcgc cgcggcaggc agcccatgac
     4441 gttgccatag gcggggtttt gggctaggct ggttccgctc aatcagggct tccggcagca
     4501 acgcggccgg aaacgatcaa taaacaaccc gttgtggagg agatgacatg aagaagctgc
     4561 tcgctgcaat cgtagccggg atgttcgcgc tgaccgcggc gacaccgctg atcgcgcagg
     4621 acaagaagaa agtcgaaaag aaggcaacga agacggagat gaccgccgag aagaaggaag
     4681 ccaaagccac gcagaaaaag ggcgagcaaa agaagatgac gaagggcgca ggcaagaaga
     4741 ccgaaggcaa gaaaacggcg aagaagggcg cagacacgaa gaaagaaaaa taatccggga
     4801 tccggagcgt gtccggaaag ggcagggtct tcaccctgcc ctttttgttt gtgcgccctc
     4861 ctgggcttgg gcgcacccgc gcgaagtaat acaattgccg ctttttccga tgcctccatg
     4921 tggaagccca gcgtaaccgt ggcagccgtg gccgaacgcg acggccgctt cctgctcgtc
     4981 gaggagcaca ccgcacatgg gatcctcttc aaccagccgg ccggccacct cgatcccggc
     5041 gaatccctgg tcgagggcgt cgcgcgcgag acgctcgagg aaaccgccca tattttcaaa
     5101 ccaacggggc tgctcggggt ctatcagtac cattccaggg cggatgacgt gacctatatc
     5161 cgttttgcct tcatcggcga aataaccggg cacgacgccg cccgcgcgct cgacgagggc
     5221 atcatctgca ccgtgtggct cacgcccgag gaaatccgcc gcgaaacggc ccgccatcgt
     5281 tcgccgctgg tcatgcgttg catagacgat tacctcgccg gccggcgcta tcccctggac
     5341 gtcctctacc gccatccctc atagaaccat gacaggaaag cgggtcatcg tcggcatgtc
     5401 gggcggggtg gactcctccg ttgcggcgct gctgctcaag cagcagggcc acgaggtctc
     5461 gggcctgttc atgaagaact gggaggacga cgacaccgac gagtactgct cctcgcggga
     5521 ggacctggtc gatgccgtcg cggtcgccga ccggatcggg atcgagatcg acgtcgtgaa
     5581 cttctcggcc gagtacaagg cgcgcgtgtt caacgacttt ctgcgcgaat accaggccgg
     5641 ccgcacgccc aaccccgacg tgctgtgcaa cgccgagatc aagttcaagg ccttcctcga
     5701 tcacgccctc ggcctcggcg ccgattgcat cgccaccggc cattacgccc aaatccgcga
     5761 ggtggacggg ctctatcaac tgctccaggc cgaggacggc accaaggatc agagctactt
     5821 cctctaccgc ctcaaccaat cgcagcttgc caggacgctg ttcccgctgg gcaggctcca
     5881 gaagcgggag gtgcgcgaga tcgcgcggcg cgagggattg cccaatcacg acaagaagga
     5941 ctcgaccggc atctgcttca tcggcgagcg gccgttccgg gagtttctca accgttatct
     6001 tcccgacaac ccgggggaga tccgcaccac ggggggcagg ctgctcggcc gccacaacgg
     6061 cctgatgttc tacaccctcg gccagcgcca ggggctcggc atcggcggca gccgggggag
     6121 cgacggcgag ccgtggtacg tcgctgccaa ggacctgccc cacaaccggc tgatcgtggt
     6181 ccagggccac gatcatcctg ccctcttgtc cgaccgcttg accgccgtgg acctcaactg
     6241 gatcagcgga acggtacccc actgccaatg ggtgtacacc gcgaagacgc gctaccgcca
     6301 gaaggacgct ccctgcacgc tcgtcgccgc cggcgtcgag cgctgcgtga tcgaattcgc
     6361 cgagccgcaa tgggcagtca cgccgggcca gtcggtggtg gtttacgaaa gccaggtctg
     6421 cctcggcggg ggcatcatcg agtcggcgga gcgcgcacgc gacgatggcg agggagagga
     6481 gaaatgtccc cccgggtcgg tggacatgaa ggatgaagga tgagaaagga gtgtcgagga
     6541 ttgagtgtcg agggaagaaa caaggggcga ggatggaggg tcgaggggga aaaccactcg
     6601 tcactcgaca ctcgacaccc gtcactcgca ctcgacaccc gtcactcgtc actgttcatt
     6661 ttcatgaata tttcgcgcat tgactcttgc ggaaagcggg tagtactctt ggaacaaatg
     6721 tattcctggt acgcgcgatg ggagcgataa gcgaaaagtt catcctcttc ctccagcccg
     6781 ggtgaagcag ctgcctccga gcgaaagagt ttctttcgaa aaagaagata gaccacgtcg
     6841 tgatcgacgt gttgaacgac gccggcggcc gcgagaagct gctcaagctc ggcgtgcgca
     6901 acgtcccggt cgtcgcgaag ggcgagaatt acgttttcgg ccagaacctg gaggacgttg
     6961 cggaattcgt cgggctacag ggcacgggac accagccgtt gccgccggat cagctcatca
     7021 agaagtgggt caacgtgctg cgcgcggcgc agcgctacat tcgccagatc ccggctcaac
     7081 gcatccatga gcgcgtcatc gataaccggg accgctcgat ccggctgctc tcccaccata
     7141 tcttccgcat cggcgaggct ttcctcgaaa gcgccatcaa cggcgtcgag tactcgatcg
     7201 ggcacgccaa tgtcccgccc aaggacggca cgttcctgac aggggccgag atcgcgggct
     7261 acggcgatga agtcatcaaa cgattgcagc agtggtgggg ctccctgaag gacccctcgc
     7321 tcgggtggaa ggtcaagacc tactatggaa tgcagcccat gcacgtcgtt ttcgagcggt
     7381 cgacctggca ctcggcgcag catgcgcggc agctaatcca cgtgctcgag cgcttcggca
     7441 tcgagcccga ctgccgcctc acaaccgagg atctcgcggg
//
LOCUS       DBNGWZ010000116         4230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3160180, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000116 DBNGWZ010000000
VERSION     DBNGWZ010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3160180"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /gene="tcuA"
                     /locus_tag="AC16CX_03460"
     CDS             complement(<1..1391)
                     /gene="tcuA"
                     /locus_tag="AC16CX_03460"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3011602.1"
                     /translation="MDYDVIVVGAGNAAFAAAVSAKENGAKRVLVLEKAPREKRGGNT
                     HFSGAVFRFIYNDVSELDRFVPNAERDYPGFHKGVQPYPVEVFREDLMRVTQGRSDPE
                     LASLLIENSYDTMCWLHDAGRHHFELAVSVMGVRVGNEVKWPRGAMIRVEHEGVGLSK
                     TWFETAEKMGIEIRYETGAQRLMQAESGRVNGVVARGPDGFVTFNARAVVLACGGFET
                     NPEWRLRYLGAPWDHAKVRGSNFNYGDGLRMALDIGAMPWGHWSGCHATPIVATASDY
                     GVQHLTDKTNRLSYFLGVMINVEGRRWVDEGADINSFTYARYGGLILKQPRSLVYQIF
                     DSKVVGLIEPRYSTSEPFKSDTLEGLVKQLMVDHKQAMKTLEEYNGAAGHGGPFNPAV
                     LDGLHTEGIEPPKTNWAVKLDTPPYVSWPVTGGITFTFGGLKVNRNAQVIATDWKPIP
                     GLYTCGEMVGGLF"
     gene            1573..2973
                     /locus_tag="AC16CX_03465"
     CDS             1573..2973
                     /locus_tag="AC16CX_03465"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011603.1"
                     /translation="MNSDRTAGLGPTRELARWVSSLRYGNLPPRTREVVRIALLDTLG
                     CGVYGYATPWAKILLQWTRAGAPAKGEATVWGETAPSLRASDSALVNGTAAHAFELDD
                     YHQAKLHAGAVVIPAAVAMAEKLGSTGERLVTAIAAGYEVMIRSSLALNPSAARLRGW
                     HLTGVCGPFGAAAACASLLGLGEEQTAWALGLAGTQGSGLWAFNADGTMSKRFHAGRA
                     AHSGVLAAELAALGFTGPTQIYEFHDGGVLKAFSDASDPAPLTRDLGKVFHLDSNSIK
                     PYSCCGSTHSYVDAALELRRRLGTPWDAKRRVRLGMARVVDVQCGFPYVPSTALNAQM
                     SLRYVVAAALMEGQALPPQFSDAKISDPVLTELAGRIECEIDPALDKLYPARFAAWIA
                     AENKEQWVRVDVLDPLGSTANPVDAQGVIEKFRGINPQLPVDDIAGAALDIEHRTVKQ
                     LLALLSGARVRQVQTA"
     gene            3010..4035
                     /locus_tag="AC16CX_03470"
     CDS             3010..4035
                     /locus_tag="AC16CX_03470"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011604.1"
                     /translation="MRNKAEVRRQKAEGSWIKVILHPSSFILGIAAALVLACAAVSAQ
                     QYPSRPIRLVVPFAPGGGTDIMARSLGQKLSEAFGQTVVVDNRAGGGGTIGTETVVRA
                     NPDGYTMIVVSGSFATNAAVYKLPYDSVNDIAPVALLGETGNMIALPLSLPIKSIKEL
                     IAADKAAPGKLNYASTGTGGFTHLITELFNQLAGTKLTHVPYKGTGPALNDLLGGQIQ
                     VLFGSLPSTIPHVKQNRLRGIGVTTPKRNNAIPDIPAIAEVVPGYEAVLWYGVWGPKQ
                     LPKNIVALWNKEIRKALKLPDMRERLANEGLDPADSPPERFSEVVRRDVAKWQKVVKA
                     GNIKAGS"
BASE COUNT          734 a         1409 c         1354 g          733 t
ORIGIN      
        1 tggaacagcc cgccgaccat ttccccgcag gtgtagagcc cggggatcgg cttccagtcg
       61 gtcgcgatca cctgcgcgtt ccgattgacc ttaagcccgc cgaaggtgaa ggtgatgccg
      121 ccggtgacgg gccacgagac gtagggcggc gtgtcgagct tcaccgccca gttggtcttc
      181 ggcggctcga taccctcggt gtgcagcccg tcgagcaccg ccgggttgaa cggtccgccg
      241 tggcccgccg cgccgttgta ttcctcgagc gtcttcatcg cctgcttgtg gtccaccatc
      301 aactgcttca ccagtccctc cagcgtgtcg gacttgaacg gctcgctggt cgagtagcgc
      361 ggctcgatca gccccaccac cttgctgtcg aagatctggt agacgaggct gcgcggttgc
      421 ttgaggatca gcccgccgta gcgggcgtag gtgaacgaat tgatgtcggc gccctcgtcc
      481 acccagcgcc taccctcgac attgatcatc acgccgagaa agtacgacag ccggttggtc
      541 ttgtcggtga gatgctgcac gccgtaatcg gaggcggtgg cgacgatggg cgttgcatga
      601 cagccgctcc agtggcccca cggcatggcg ccgatgtcga gcgccatcct gagaccgtcg
      661 ccgtaattga aattggagcc gcgcactttg gcatggtccc acggcgctcc gagatagcgc
      721 agccgccatt ccgggttggt ctcgaagccg ccgcaggcga gcaccaccgc tcttgcgttg
      781 aacgtgacga agccatccgg cccgcgcgcc acgacgccgt tcacgcgtcc gctttccgcc
      841 tgcatgagcc gctgcgcgcc agtttcgtag cggatctcga tgcccatctt ttccgcggtc
      901 tcgaaccagg ttttggacaa gcccacgccc tcgtgctcga cgcggatcat cgcgccgcgc
      961 ggccacttca cctcgttgcc gacgcgcacg cccatcaccg acacggcgag ctcgaaatgg
     1021 tgcctgcctg cgtcgtgcag ccagcacatc gtgtcgtagg agttctcgat gaggagcgag
     1081 gcaagctccg ggtcgctgcg gccctgcgtc acgcgcatca ggtcctcccg gaacacctcc
     1141 accggatagg gctgcacgcc cttgtggaaa cccgggtagt cgcgctcggc gttcggcacg
     1201 aaacggtcga gctccgagac gtcgttgtag atgaagcgga acaccgcccc cgagaaatgc
     1261 gtgttgccgc cgcgcttttc ccgcggcgcc ttctccagca ccagcacccg tttcgcgccg
     1321 ttctccttcg cggacaccgc ggcggcaaaa gccgcgttgc cggcgccgac cacgatcacg
     1381 tcgtaatcca ctgaaatctc cccgatgcgt aagccgtaag cggtgagcgg taagcggaaa
     1441 gcagtaagca agaagtgcta aatcggactt gtgttgtcgc gaacgtttac cgcttacggc
     1501 tcaccgctta ccgcttgcgg ttgttagaat acgcgcactg ccgcatcacg cgccaccgcc
     1561 ggagcccata tgatgaactc cgatcgaact gccggtcttg gcccgacgcg cgagctcgcg
     1621 cgctgggtgt catcgttgcg ttacggcaac ctcccgccgc gcaccaggga agtcgtccgc
     1681 atcgccctcc tcgatactct gggttgcggc gtctacggct acgcgacgcc ctgggcaaaa
     1741 atattgctgc aatggaccag ggcgggcgcg ccggcgaaag gcgaagcgac cgtgtggggc
     1801 gaaacggcgc cgtccttgcg cgcgtccgac tcggcgctcg tgaacggcac cgccgcgcac
     1861 gccttcgaac tcgacgacta tcaccaggcg aagctccacg ccggcgcggt ggtgattccc
     1921 gccgcggtcg ccatggcgga aaagctcggt tccaccggcg agcggctcgt gaccgccatc
     1981 gcagcaggct acgaagtgat gattcgctcg agcctcgccc tgaatccctc cgccgcgcgg
     2041 ctgcggggat ggcacctgac cggcgtgtgc gggccgttcg gcgccgcggc ggcgtgcgcg
     2101 tcgttgctgg gactcggcga ggaacagacg gcctgggcac tgggactcgc gggcacgcaa
     2161 gggtccggat tatgggcgtt caatgccgac ggcacgatga gcaagcgttt ccatgccggc
     2221 agggccgcgc attccggcgt gcttgcggcc gagctcgccg cgctcggctt caccgggccg
     2281 acgcagattt acgagtttca cgacggcggc gtgctgaagg cgttctccga cgcctccgat
     2341 cccgcgccgc tcacgcgcga tctcggcaag gtcttccatc tcgactcgaa ctcgatcaag
     2401 ccctactcct gctgcggcag cacgcactcg tacgtggacg ccgcgctcga gttgcggcgc
     2461 cgcctcggca cgccgtggga cgcgaagcgc cgcgtgcgcc tgggaatggc cagggtggtg
     2521 gatgtgcaat gcggctttcc gtacgtgccg agcaccgcgc tgaacgcgca aatgagcctg
     2581 cgctacgtgg tcgcggcggc gctcatggag ggccaggcgc tgccgccgca gttcagcgat
     2641 gcgaagatct ccgatccggt gctgacggaa ctcgccggcc gcatcgagtg cgagatcgat
     2701 cccgccctcg acaagctcta tcccgcgcgc ttcgcggcct ggatcgcggc ggagaacaag
     2761 gagcaatggg tgcgggtgga cgtgctcgat cccctcggtt ccaccgccaa tccggtggac
     2821 gcgcagggcg tgatcgagaa attccgcggg atcaacccgc agttgccggt ggacgatatt
     2881 gcgggcgccg cactcgatat cgagcaccgt acggtcaagc agttgctcgc tcttctctcc
     2941 ggggcgcgag tcaggcaggt ccagacagcg tgacggcgcc cacgagggat gagggcgaag
     3001 ggatgaggga tgaggaataa ggcagaagtc agaaggcaga aggcagaagg aagctggatc
     3061 aaggtcatcc ttcatccttc atccttcatc cttggtattg ctgcggcgct ggtgctggcg
     3121 tgtgccgccg tcagcgcgca gcaatacccg tcgcggccaa tccggctggt ggtgccattt
     3181 gcgccgggcg gcggcaccga tatcatggcg cggagcctgg gacagaagct gagcgaggcc
     3241 ttcgggcaga ccgtcgtggt ggacaaccgc gccggcggcg ggggcaccat cggcaccgag
     3301 accgttgtca gggcgaaccc ggacggctac accatgatcg tggtttccgg cagcttcgcg
     3361 acgaacgcgg ccgtgtacaa gctgccatac gattccgtca acgatatcgc gccggtcgcg
     3421 ctcctcggcg aaactggaaa catgatcgcg ctgccgcttt cgctgcccat caagagcatc
     3481 aaggagctga ttgcggccga caaggccgct cccggcaagc tcaattatgc ctcgaccggt
     3541 accggcggct tcacccacct catcaccgag ctcttcaacc agctggcggg cacgaagctc
     3601 acgcacgtgc cgtacaaggg caccggtccg gcgctgaacg acctgctggg cggccagata
     3661 caggtcctct tcggcagcct gccttcgacc atcccgcacg tgaagcaaaa ccggctgcgc
     3721 ggcatcggcg tcaccacgcc caagcgcaac aatgccattc ccgacattcc tgcgatcgcg
     3781 gaagtcgtgc ccggctacga agcggtgctg tggtacggcg tctgggggcc gaagcagctg
     3841 ccgaagaaca tcgtggcgct gtggaacaag gagatccgca aggcactgaa gcttccggac
     3901 atgcgcgagc ggcttgccaa cgaaggcctc gatccggccg actctccgcc cgagcgcttc
     3961 agcgaagtcg tcaggcgcga cgtggcgaag tggcagaagg tggtcaaggc gggaaacatc
     4021 aaggccggga gttgagccgt aagccgtaag ccgtaagccg taagccgtca attggacttt
     4081 tgctttggcg aacgtttcgg gcccattgct cacgcttcgc ggttccccgc tcactgccca
     4141 ccgctcaccg cttaccgcta accgctcacg gtatttctcc tcaccgctta ctgctcaccg
     4201 tctcacgtat gaagctcgac gatgtccccg
//
LOCUS       DBNGWZ010000117         2474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_389127, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000117 DBNGWZ010000000
VERSION     DBNGWZ010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_389127"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16CX_03475"
     CDS             complement(<1..224)
                     /locus_tag="AC16CX_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011605.1"
                     /translation="MRGEEKKLLDLFERLTPEQQDRLIAFAEFLTVGAADEATTASEP
                     LAIPRPPGETVTMAIRRLVRTYPMLNRRKL"
     gene            complement(265..1173)
                     /locus_tag="AC16CX_03480"
     CDS             complement(265..1173)
                     /locus_tag="AC16CX_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3011606.1"
                     /translation="MADRRLQVFYTVARHLSFTKAAEQLFMTQPAVTFQIKQLEEHFN
                     ARLFERSHGKIALTPAGRLVMDYAERILGLSEEMETRVAELSGAMSGPLLLGASTTIA
                     EFVLPPILGEFTVEHPQVHAHLTVGNSEMIENRVADHTLDLGIIESPSHLPNLNTSVW
                     CEDELVLICSPAHKFAKSASVKPQDLVGEPYVSRELGSGTREFTDQYLQGCNVAPEDL
                     NVVMELGSPEAIKSMVETGLGVSIMSRARVAKERKLGTLVAVPLGPRLIRTFSFVQPK
                     DKFRSKLLVAFIEFADTRVKKLADGG"
     gene            1513..>2474
                     /gene="lplT"
                     /locus_tag="AC16CX_03485"
     CDS             1513..>2474
                     /gene="lplT"
                     /locus_tag="AC16CX_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HIR3011607.1"
                     /translation="MPFGFYIIMAAQFFSSLADNALLVAAIALLIQMAAPEWMTPLLK
                     FFFTVSYVAFAAFVGAFADSMPKGRVMFITNLVKIVGCLLMFVSVWLPSSDGSLYIPV
                     LVAYGVVGLGAAAYSPAKYGILTEYLPHQKLVIANGWIEGLTVASIILGTLLGGVLIS
                     ETVSRRLLAIDIPQIDTGIDTPAEAAILVITVFYAIAAIFNLYIPNTGVDHRAPSKNP
                     LFLAHEFAHCVKLLWSDKLGQISLATTTLFWGAGATLQFIVLKWAEVALGYTLSQATV
                     LQGICAVGIAMGAIIAAKFIPLNRTVSVVPVGIAMGLIVIVMIFV"
BASE COUNT          432 a          797 c          736 g          509 t
ORIGIN      
        1 atgagcttgc gccggttgag catgggatag gtgcgtacga ggcgtcgtat cgccatcgtg
       61 accgtttcgc cgggtgggcg cgggatggcg agcggttccg acgcggtggt tgcctcgtcc
      121 gccgcgccca cggtgagaaa ttccgcaaac gcgatcagcc tgtcctgctg ctcgggcgtc
      181 agccgttcga acagatcgag cagctttttc tcctcacctc tcactcctca cacctcactc
      241 ctcgcccctc acacctcgcc ggcactagcc gccgtcggcc agctttttca ccctggtgtc
      301 ggcgaactcg atgaaggcga ccagcagctt cgagcggaac ttgtccttgg gctgcacgaa
      361 agagaaggtg cggatcagcc gcggcccgag cggtaccgcc acgagagtcc cgagcttgcg
      421 ctccttcgcc actctcgccc gcgacatgat ggagacgccg agcccggtct cgaccatgct
      481 cttgatcgct tccgggctgc cgagctccat caccacgttg aggtcctcgg gcgcaacgtt
      541 gcagccttgc aggtactggt cggtgaactc gcgcgtaccc gagcccagtt cacggctcac
      601 gtacggctcg cccacgagat cctgcggctt gacggacgcg gacttcgcga acttgtgcgc
      661 cggagagcaa atcagaacaa gttcgtcctc gcaccacacc gacgtattca ggttcggcag
      721 gtgcgaaggc gattcgatga ttcccaggtc gagcgtatgg tccgccaccc gattttcgat
      781 catctcggaa ttgcccaccg tgagatgcgc gtgcacctgc gggtgctcca ccgtgaactc
      841 cccgaggatc ggcggcagga cgaactcggc gatggtcgtg ctcgccccca gcagcagcgg
      901 cccgctcatg gcgccggaca gctccgcgac ccgggtctcc atctcctcgg acagcccgag
      961 gatgcgctcg gcgtaatcca tcacgagccg gcccgccggc gtgagcgcga tcttgccgtg
     1021 gctgcgttcg aagagccgcg cgttgaagtg ctcctcgagc tgcttgatct ggaacgtcac
     1081 cgccggctgc gtcatgaaca gctgctccgc ggccttggtg aagctcaggt gcctggcgac
     1141 ggtatagaaa acctgcaatc ggcggtcagc catgggcgcg tgcgcttgga gtgaagagtg
     1201 aggcgcaagg agaaaaacat ttccgccagc gccaaggtgt tacttctcac tcctcgcacc
     1261 tcactcctca ctggaaaaag gttagtattc aaccacattt cgcatgatct gtggtataaa
     1321 gcggcagcct acaataagaa aagggtgaag gatgagggat gaaggatgaa tgaaaatcag
     1381 gccaagcagc tggaggttgc cataaccctc tttctgtcct cgtacttccg cctccatcct
     1441 ttacccgccc cgctttccgc attgcttctc gcattgccac accgcgctct atagcccgga
     1501 cctggactgc gcatgccctt cggcttctac atcatcatgg cggcgcagtt cttctcttcg
     1561 ctcgccgaca acgccctgct ggtcgcggcg atcgcgttgc tgatccagat ggcggcgccg
     1621 gagtggatga cgccgctgct caagttcttc ttcaccgtct cctacgtggc gttcgcggcc
     1681 ttcgtgggcg cgttcgctga ctcgatgccc aagggccgcg tcatgttcat caccaatctg
     1741 gtgaaaatcg tcggctgtct cctgatgttt gtctcggtct ggttgccgtc atccgacggc
     1801 tctctgtaca ttccggtgct ggtcgcgtac ggggtcgtcg ggctcggcgc ggcggcctat
     1861 tcgcccgcca agtatggcat cctgaccgag tacctgccgc accagaagct ggtcatagcc
     1921 aacggctgga tcgagggcct gacggtcgcc tcgatcatcc tcggcacgct gctcggcggc
     1981 gtgctgatca gcgaaacggt gtcgaggcgg ctgctcgcga tcgacattcc ccaaatcgac
     2041 accggaatcg atactcctgc ggaagcggcg atcctggtaa tcaccgtgtt ctacgcgatc
     2101 gcggcgattt tcaatcttta cattcccaat accggggtgg accaccgtgc gcccagcaag
     2161 aacccgctgt tcctggcaca tgaattcgcg cattgcgtga agctgctgtg gagcgacaag
     2221 ctggggcaga tctcgctcgc caccacgacg ctgttctggg gagcgggcgc gacgttgcag
     2281 ttcatcgtgc tcaaatgggc tgaagtggcg ctcggttaca ccctgtcgca ggcgaccgtg
     2341 ctgcagggaa tctgcgccgt cggcatcgcc atgggcgcca taatcgccgc aaagttcatc
     2401 ccgctcaacc gcacggtcag cgtggtcccg gtcggcatcg cgatgggact gatcgtgatc
     2461 gtgatgatct tcgt
//
LOCUS       DBNGWZ010000118         1658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3411788, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000118 DBNGWZ010000000
VERSION     DBNGWZ010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3411788"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..566)
                     /gene="soxY"
                     /locus_tag="AC16CX_03490"
     CDS             complement(99..566)
                     /gene="soxY"
                     /locus_tag="AC16CX_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HIR3011608.1"
                     /translation="MNVYRRTFLKLTGGMGALAAVFAAGLVEAGRAWAAEWNQAGFSS
                     KAMADALKSLGAANPVESKDILITAPDIAENGAVVPVAVTSKLPNTQQISIVAEKNPF
                     PLAATFDVAGGGEGYVSTRIKMGQTSNVWAVVKANGKFYTAKKEIKITVGGCG"
     gene            1009..>1658
                     /locus_tag="AC16CX_03495"
     CDS             1009..>1658
                     /locus_tag="AC16CX_03495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3011609.1"
                     /translation="MFCARNLAPEASMDIDIFLEFASPPMAGCRMTQVFADGLALARS
                     ADAAGFGAVWLAEHHFLGDYSNASAPELLLAAMARETRRVGLGFAVVPLTLHDPVRVA
                     ERLATLDLVSGGRVLWGVGRGVTPVELDAFGVEPARSRAEFRRRYAELASILRTGRIE
                     RAGRVLEIRPQPSPRLIDGWMATVSPESYDLAAELGLDVMAGPFKPWPMVKADLAR"
BASE COUNT          243 a          551 c          500 g          364 t
ORIGIN      
        1 cgcacgtcgg cgacgtcgcc ttgcagcgta gcgcggatct tcatcggttc agccatggct
       61 taagtccttt cacagatttc cagttggcca aagtccgatc agccgcatcc gcccacggtg
      121 atcttgattt ctttcttcgc ggtgtagaat tttccgttcg ccttgaccac tgcccacacg
      181 ttggaggtct ggcccatctt gatgcgggtc gagacgtagc cctctccgcc gccggcaacg
      241 tcgaacgtcg ccgccagcgg aaacgggttc ttctcggcga cgatcgagat ctgctgggta
      301 ttgggcagct tgctggtcac agcgaccggc accaccgcgc cgttttcggc gatgtccggc
      361 gcggtgatca ggatgtcctt gctctcgacg gggttggctg cgcccaggct tttcagggcg
      421 tccgccatcg ccttggagga gaatcccgcc tggttccact ccgcggccca cgcccggccg
      481 gcttcgacca gtccggcggc aaagacggct gccagcgcgc ccattcctcc ggtcagtttc
      541 aaaaacgttc tgcgatatac gttcatggac gttctttcct cctgccttga gtggaaatac
      601 gcgaggttgc tgcctgttct tgaagaatgt ttattctact ttcttgaccc tgtctactga
      661 aggggtttta tcggcctata aaaaaacatt tttccgcagc ctgcgccggg cgttgtactc
      721 gtcggcagcg gtcgaaacag cggccaggtc gctcatccta cgatgcctct gatgctcatt
      781 gcactgatta tacgtgtaga gctgttgagc atgctcgatg ctcctccggg ggccgcaacc
      841 cgcttctggt acaaaccgcg ggcggccggt cgcggccgcg ctcaacgact tcctttcgac
      901 gccggcgcgc tccacgttct caaccgtggc catgatcgcg gctccgccgt cgcggctttt
      961 tcctcgatag cgataggttg atctcgaatt tgaatgaagc gagaatgaat gttctgcgct
     1021 cgaaaccttg ccccagaagc atccatggac atcgacatct tccttgaatt tgcctcgcct
     1081 cccatggcgg ggtgcaggat gacgcaggtc ttcgcggacg gccttgcgct cgcccgctcc
     1141 gccgatgctg cgggcttcgg cgctgtctgg ttggccgagc accactttct cggcgattat
     1201 tcgaacgcct ccgccccgga gctgctgctc gccgcgatgg cccgcgagac ccggcgcgtt
     1261 ggcctcggct ttgccgtggt tccgctgacc cttcacgacc cggtgcgcgt tgccgagcgc
     1321 ctcgcgacgc tggaccttgt ctccggcggc cgcgtcctgt ggggcgtcgg gcgcggcgtg
     1381 acgcccgtcg agctcgatgc cttcggcgtc gagcccgcgc gcagccgggc cgagtttcgc
     1441 cgccgctacg ccgagctcgc cagcatcctc cgcacgggca gaatcgagcg cgcggggcgg
     1501 gtgctggaga tccgtccgca gccatcgccc cggctcatcg acggctggat ggcgaccgtg
     1561 agtcctgaga gctacgacct tgccgccgag ctcgggctcg atgtgatggc cggtccgttc
     1621 aagccctggc ccatggtgaa ggccgacctc gcccgcta
//
LOCUS       DBNGWZ010000119         1810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2152581, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000119 DBNGWZ010000000
VERSION     DBNGWZ010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2152581"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1810
                     /locus_tag="AC16CX_03500"
     CDS             <1..>1810
                     /locus_tag="AC16CX_03500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3011610.1"
                     /translation="TGRGVLAQALKTRKPVFDNDITINPEMGGARRQEAIRRGYRSFI
                     VLPLVVETSVFGSLSLLAKEANFFDEEELRLLTELAGDISFALEHIAGQERIERLSRI
                     RAVSSSINAAIVRIRERQALFEETCRIASEEGKFEMVWIGTIDAEKQKVEAVTWTGFS
                     EAAARAVSWASVSAAQGTLGEATRTRRPAVRNDIETGLTTGKLRQEALQRGCLSTVCL
                     PLVVGERVSALIALFAPGRGFFDEGELALFEEVAADVSFALDHIEKEEQLNYLAYYDV
                     LTGLPNRTLFNDRLGQRIGAARDEKQSFSVMMLNVERFRLINDTFGRQVGDELLRGIA
                     RRLKSVLHGADLLAHLGGDQFAIATHRAGEPGIIAHVLEQVMTSVLGEPFNAGSGELH
                     LAARAGVAVYPSDGADVETLVRNAEAALNDARKTGQRHVFYAPQMNARIAVQLKLENE
                     LRRAVQEEQFVLFYQPRFELSDGRITGLEALIRWKHPERGLVPPGEFISLLEDTGLIV
                     EAGRWVLGRAALDHTAWQSKGFIPPRIAVNVSQVQLQRKDFVDYVKDALEGMDKAAER
                     LDIEITESMLMEDIEGTIGKLQAMQAMGLHIAVDDFG"
BASE COUNT          349 a          530 c          621 g          310 t
ORIGIN      
        1 cgaccggtcg gggcgttctg gcgcaggcgc tcaagacacg aaaacccgtc ttcgataacg
       61 acatcacgat caatcccgag atgggcggag cgcggagaca ggaagcaata agacggggct
      121 accggtcatt catcgtgctt cctctcgttg tcgaaacgag cgtttttggc agcctgtcgt
      181 tactcgcgaa ggaagccaac ttcttcgacg aggaggaact gaggctcttg accgagctcg
      241 ccggtgacat ttccttcgcg cttgagcaca tcgccgggca ggagaggatc gagaggctct
      301 cgcgcatccg tgcggtatcg agcagcatca acgccgccat cgtccgcatc cgcgagcgcc
      361 aagccttgtt cgaggagact tgtcgcatcg cgtccgagga ggggaagttc gagatggtgt
      421 ggatcgggac gattgacgcc gaaaaacaga aggtcgaggc cgtcacctgg acggggttct
      481 cggaggcggc agcacgcgcc gtgagctggg cgagcgtcag tgcggcgcag ggaacgctgg
      541 gtgaggcgac ccggacccgg cgaccagcgg tccgcaatga catcgaaacg gggctcacga
      601 cgggtaagct gcgccaggag gcgttgcaaa ggggctgtct ttccacggta tgcctgcccc
      661 tggtcgtggg tgagcgcgtg tccgcgctca tcgcgctttt tgcgcccggg cgcggcttct
      721 tcgacgaggg tgaactcgcc ttgttcgagg aagtggcggc ggacgtctcc ttcgcgctcg
      781 accatatcga gaaggaggag cagctcaact acctggcgta ctacgacgtc ctgaccggct
      841 tgccgaaccg cacgctgttc aacgaccgcc tcgggcagcg catcggcgcg gcgcgggatg
      901 aaaagcaatc attttcggtc atgatgctca acgtcgaaag gttcaggctc attaacgaca
      961 cgttcggccg gcaggtaggc gacgagctgt tgcgagggat tgcgcggcgc ctgaagagcg
     1021 tcctccacgg cgcggacctt ctggcgcacc tcggcggtga ccaattcgcg atcgcaacgc
     1081 accgcgccgg ggaaccgggg atcatcgcgc acgtgcttga gcaggtcatg acgagcgttt
     1141 tgggggagcc gttcaacgct ggcagcggcg agctgcatct cgcggcccga gcgggcgtcg
     1201 cggtctaccc ctcggacggg gcagacgtgg aaactctggt caggaacgcc gaagccgcgc
     1261 ttaacgacgc cagaaagacg gggcagcgcc acgtgtttta cgcgccgcag atgaatgccc
     1321 gcatcgccgt gcagttgaag ctcgagaacg agctgcgccg cgcggtgcag gaagagcagt
     1381 tcgtgctgtt ttatcagccg cgcttcgagc tttccgacgg gcgcatcacc ggcctcgagg
     1441 cgctgatacg gtggaagcac ccggaacgcg gactggttcc gccgggcgag ttcatttcac
     1501 tgctggagga tacggggctg atcgtcgagg ccgggcgctg ggtgctgggg cgcgcggcgc
     1561 tcgatcacac cgcatggcaa tccaagggat tcataccgcc gcgcattgcc gtcaacgttt
     1621 cccaggtgca gttacagcgc aaggatttcg tcgattacgt gaaggacgcg ctcgagggaa
     1681 tggacaaggc ggcggagcgg ctcgacatcg aaatcaccga gagcatgctc atggaggaca
     1741 tcgagggcac cattggcaag ctgcaagcga tgcaggccat gggcctgcac atcgccgtcg
     1801 acgacttcgg
//
LOCUS       DBNGWZ010000120        29321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1522903, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000120 DBNGWZ010000000
VERSION     DBNGWZ010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 29321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 29321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29321
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1522903"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16CX_03505"
     CDS             complement(<1..534)
                     /locus_tag="AC16CX_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3011611.1"
                     /translation="MKPDLPVWRSLLYVPVNVEKFIEKAHTRGADVIQLDLEDAVPPA
                     EKEKARTLVARNAARVRRGGADVVVRINQPLSLAVRDLEHSVGPDVDGIAITKVDSAS
                     HVKLLDELVSGLEEKRGMKAGHTRFIVMIETADAFTRIDEIPRASPRVVAMNIGGEDF
                     ALDLNAQPDDDVLLHPKQ"
     gene            complement(595..1854)
                     /locus_tag="AC16CX_03510"
     CDS             complement(595..1854)
                     /locus_tag="AC16CX_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011612.1"
                     /translation="MTAALPRKSYDPAATGPLTGVRVLDLSRLVAGNTLTQVLGDFGA
                     EVIKIEPPAGDTLRAWQTSGVQTNWKLYARNKKSLGLELRKPEARDLLARLLPSAAIL
                     VESFRPGTLEKMGFGPDRLLGFNPKLVIVRISGWGQDGPYQRRPGFGTLVEGMSGFAS
                     FNGFADREPVLPPMYLADTVAGLYGAAAAMIALREVEQNGGRGQVIDLPLLDPLFAVL
                     GPQAANYRLTGKVKPRTGSRSTNAGPRNAYRCKDGLYVCLSSSTQKMAERLFRSIGRP
                     DLIDDPRYRTNAERVKHADDLDVIIVEFVAQRTQAENVAFFEQAEVTIGPIYDIRQIL
                     EDPHVIARELVADYPDADMGAFPMHHVVPRLSGTPGAIRTPAPRLGEHNRQLLAEIGV
                     DDAAYSQLLATGVACEGGNPPAVSGEQ"
     gene            complement(1851..2810)
                     /locus_tag="AC16CX_03515"
     CDS             complement(1851..2810)
                     /locus_tag="AC16CX_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011613.1"
                     /translation="MIRAACLALGALAFAGAAPAQPYPAKPIRLVVPYPPGGSNDVLS
                     RITAQAMSPGLGQPVVIDNRSGAGGMIGADHVAKSVPDGYTLVNVQASFTANAALRAK
                     MAYDPLGDFAYIGMMARGPLLAVVHPSLPVKNVKELIALARAKPGQINYGSTGTGGHN
                     HLATELFARMAGVKIVHVPYKGVAPALTDLMGGHTQLVMTSLPSAMTQVQAGRLKALA
                     VGSEQRSSFMPELPTISESGVPGYFAEFWWGIAAPSKTPNDVLNRLASEVAKALQSAD
                     LKQRFAAEGAEPSPMTREQFSRFVANEITRWRKVALEASIRPE"
     gene            complement(2813..3616)
                     /locus_tag="AC16CX_03520"
     CDS             complement(2813..3616)
                     /locus_tag="AC16CX_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3011614.1"
                     /translation="MPRKTEAGAGNRLTGTQSIERSLMLLREIAAHNRGGSRLLDLAT
                     RTGLQRPTVHRMLKCLATENMVQQDPDTHRYFLGPMVFELGLTATPRFNLREICHPAL
                     TRIAEATGDTVFLTQRSGLDSVCLDRREGTFPIKTFTLEIGMRRPLGVGTGSLAILSA
                     LPEEEIQKVVGANAGRLPEYGLNTGALFAQVKRSQKLGYAMRDVPGLAGVRSVAQALR
                     NQSGIAFAALSVSTISSRMSDKRATEVAQLLKGESRQIERQLASGAEHR"
     gene            3793..4710
                     /gene="prpB"
                     /locus_tag="AC16CX_03525"
     CDS             3793..4710
                     /gene="prpB"
                     /locus_tag="AC16CX_03525"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HIR3011615.1"
                     /translation="MNERTSSTAPAGSAGARFRAALAEERPLQVIGTINANHARMAER
                     VGYRAIYLSGGGVAAGSLGVPDLGISGLDDVLTDVRRITDVCPLPLLVDVDTGFGASA
                     FNIARTVKSLIKFGAAAMHIEDQVGAKRCGHRPNKELVSQQEMVDRIKAAVDAKTDPA
                     FVVMARTDALAVEGLQAGIDRACACVEAGADMIFPEAITELAMYRKFADAVKVPILAN
                     ITEFGQTPLYTVKELGTAGVAMVLYPLSAFRAMNAAALRVYQAIRKDGTQKGVLELMQ
                     TREELYDFIGYHAYERKLDELFTRDKKNK"
     gene            4915..6168
                     /gene="hemA"
                     /locus_tag="AC16CX_03530"
     CDS             4915..6168
                     /gene="hemA"
                     /locus_tag="AC16CX_03530"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR3011616.1"
                     /translation="MQLFAFGINHQTAPLSVREQVVFHAENLVGALRELVDHRPVKEA
                     AIISTCNRTEVYCNTEDPRAAVDWMAGYHGFKPQQIEQYLYKLPQERAVKHAFRVASG
                     LDSMVLGEPQILGQFKQAVRTAQAAGTLGLLLNKLFQRTFAVAKTVRSETEIGAATVS
                     MASAAVGLAQRIYPSIVGQNVLFIGAGEMIELTATHFAAQHPKRLTFANRTLERAQVL
                     ADRFLGRAITLNELPSQLASHDIIISCTASTLPIIGKGLVESALRARKHRPMLMYDLA
                     VPRDIEGEVGALDDVFLYTVDDLGKIAREGLDVRSNAVAQAEVIIENQVTDFMHWLGN
                     RELVPTIRALRDNAERARRHEMERALRRLAKGEDPKAVLEQLSHALAAKLTHAPTHAL
                     SHARDEDRDQLAQLLTRLYQIPRPE"
     gene            6209..7300
                     /gene="prfA"
                     /locus_tag="AC16CX_03535"
     CDS             6209..7300
                     /gene="prfA"
                     /locus_tag="AC16CX_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR3011617.1"
                     /translation="MKPSIAAKLSQLSGRLEEINRLLALENATADLDQYRKLTREHAE
                     IAPVVELYQQYVKGEADIQAAQEMSGEPEMREFAESEIRQTRERLAGIEQELQKKLLP
                     RDPNDERNIFLEVRAGTGGDESALFAAELFRMYSRYAERNRWQVEVISQSPSDLGGYK
                     EIIAKISGQGAYSRLKFESGGHRVQRVPVTETQGRIHTSACTVAVMPEADEIADVVLN
                     PAELRVDTFRASGAGGQHVNKTDSAVRVTHLPSGIVVECQDDRSQHKNRARALAILAA
                     RIKDRQIREQQARLAATRKSLIGSGDRSERIRTYNFPQGRVTDHRINLTLYKIGQVMD
                     GELDELISALAAEHQAEQLAQLAEETHPV"
     gene            7313..8194
                     /locus_tag="AC16CX_03540"
     CDS             7313..8194
                     /locus_tag="AC16CX_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3011618.1"
                     /translation="MAFPAEVSLQAVTFAVLSALGFGAALVTTHSGLKHVDATTGSKV
                     SIPTATLLFWLLSPFLDWTGWQTAAIGIFALVGLFFPAAVTLLTFEANRRLGPTVTGT
                     IGSTAPLFAVLGAAAFLGEPLGVRALVATALIVLGSIALSRPQVADTETRQSGTLWLP
                     WSAAALRALAQVLSKAGLALWPNPFAAALVGYTVSSAVVWAAAFDRRAPAPVFNRHGI
                     AWFAVTGALNGAAVLFLYCALNNGPVYVVSPIVATYPLFTMMLSVLALREERLNGALI
                     GGVALTVTGVVILLARW"
     gene            8206..9024
                     /gene="prmC"
                     /locus_tag="AC16CX_03545"
     CDS             8206..9024
                     /gene="prmC"
                     /locus_tag="AC16CX_03545"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3011619.1"
                     /translation="MAGASIDEMLRSAGIDPVDARVLMRAALGVSDAHLAAHPEQALS
                     RDERDRYLGWVERRHAGAPVAYLTGEREFYSFAFKVTPAVLIPRPETELLVETALEHI
                     PVDEPCRVLDLATGSGCVSVAIAKHRPRARVTATETSREALALARENAARHGAAVEFV
                     ESGWFEALSGRRFEMIVANPPYIAAGDPHLNAGDVRFEPRDALVAGPTGLECIEVIVE
                     GAARYLTAGGGWLLFEHGYDQGACSRALLAAAGYEDVVTRRDLAGIERVSGGKV"
     gene            9088..9405
                     /gene="grxD"
                     /locus_tag="AC16CX_03550"
     CDS             9088..9405
                     /gene="grxD"
                     /locus_tag="AC16CX_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR3011620.1"
                     /translation="MSIQDVIKNQVSKNRVVLYMKGTPDFPQCGFSANAVGILHACGV
                     DEFFSVNVLENPDIRQGIKEYANWPTIPQLYVNGEFVGGSDIMTEMYKSGELKKLLEK
                     TAA"
     gene            complement(9522..10292)
                     /locus_tag="AC16CX_03555"
     CDS             complement(9522..10292)
                     /locus_tag="AC16CX_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3011621.1"
                     /translation="MPLEGFSAGLIAWMACVVLVAGLSQGALGFGFPFIATPLIAMVT
                     DIRTAVIAVLLPTLATITIALVTSGPIRATLARFWMMPLYAVAGSAAGTWLFVSAPDV
                     PYTLLLALMTLAYLNLDRLGRAEWPLVRRHERAFAPLSGLAAGIFEGTANIAAPPLII
                     FYLALHLPPVMLVQALNICFLVGKTTQFTVLTAGGGVTAEQWFATLPLVVIGVVGSLT
                     GVRIRNRIDARAFRIWVKRALFVIALVLLGQNAWSLFA"
     gene            10545..11309
                     /locus_tag="AC16CX_03560"
     CDS             10545..11309
                     /locus_tag="AC16CX_03560"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR3011622.1"
                     /translation="MSQPNPFLSSGSNPSSSAGPADGGRAVDAGQGWAWIAGGFDLFK
                     RKAGIWIALIIVLAVILIVMSFIPFLGQLAMFLLLPVFTGGLMLGCQALERDGSLEIG
                     HLFAGFKAHTGSLIVLGVFGLVATIVIMLPVIAIVGGGAFFGAMRGDAAGIGAIGASF
                     ALAFLIAFGLSILVYMALWFAPALVVLRGLAPMEAVKQSFLGCLKNIIPFLIYGIIML
                     VLSVIASIPLALGWLILGPVLVASVYVAYRDIYPAS"
     gene            11532..13298
                     /gene="xsc"
                     /locus_tag="AC16CX_03565"
     CDS             11532..13298
                     /gene="xsc"
                     /locus_tag="AC16CX_03565"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HIR3011623.1"
                     /translation="MTEAKNLTTSNKPASGKVRMSPSEAFIETLAAHKVKDVFGIVGS
                     AYMDALDLFPGAGIRLYSVAHEQNAAHMADGYGRVTNRPSVCIAQNGPGITNFVTAVA
                     AAYWAHTPMVVVTPESGSLTAGHGGFQETEQMPIFSKITKWQVHVNSPLRMAELMHRA
                     FTLALHERGPVQVNIPRDYFYGEGDYEILPPLVIERAAGGSNSLDRAVKMLAEAKFPV
                     IMSGGGVVMSDGVDEVKALAEHLTAPVVNTYLHNDSFPAAHPLSCGPLGYQGSKAAMR
                     IISRADVVLALGTRLGPFGTLPQHGIDYWPKDAKIIQIDADHRTLGLVKKASLAIQGD
                     ARLAAAELLKRLKARGARKPDKARLVEVQREKEAWAAELANWPSPNKKGQIGPRRALA
                     QLAKALPRNAMVSTDIGNVCSVANSYLRFEQPLSFLAAMSWGNCGYAYPTALGARVAR
                     PDRPAIAYVGDGAWGMSLAEVMTAVKEKIPAVAVVFNNGQWGAEKKNQIDYFDNRFVG
                     TNLTNPDFSEVARVMGAEGYRVEHEDEVSDALRAALKSGKPAVVEIMLTQELGEPFRR
                     DAFRKPRRLLPKYRRYSAKEYR"
     gene            13385..14416
                     /locus_tag="AC16CX_03570"
     CDS             13385..14416
                     /locus_tag="AC16CX_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3011624.1"
                     /translation="MNQGPGSKSAKLSDVARLARVSTATVSRALTQPDKVKPATAARI
                     RQAVQALGYVAHGAARALASRRTHTIGAVIPTLDNAIFANTTHALQKTLDEAGYTLLL
                     ACHEFEARAEARMTQALIERGVDGLVLLGVSHHPSVFQMIDTHRIPYVLTWALDASGR
                     HPCVGFDNRMAAIRMAGHLLELGHREFAMISGVTAGNERATGRLQGVRQALAERAIVL
                     APECIVEKPYTLSAGREGLREVLRGTPRPTAVICGNDVLAIGALAECHAQGLAVPREI
                     SVTGFDDLEMAAVVTPALTTVHFPTAELGSYAAQHLLARLAGKPVEMRTELPVELVVR
                     ASTAPPAIT"
     gene            complement(14406..15293)
                     /locus_tag="AC16CX_03575"
     CDS             complement(14406..15293)
                     /locus_tag="AC16CX_03575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR3011625.1"
                     /translation="MSPWQDIERSIREASGAPFTIESRAGAGGGCINECHVVRGQGRA
                     FFVKLNAPDKAEMFSAEAAGLEEIGRTKTVRVPRPVCHGACPAASWIVLEHLELRPAD
                     DRSMRALGRNLARLHRVTCGRYGWHRDNTIGSTPQTNTPADDWIAFWRDRRVRFQLGL
                     AASKGHGGRLIANGERLMEKLPAFFAGYHPDPSLLHGDLWSGNVAMDAGGEPVIFDPA
                     VYYGDREADLAMTELFGGFPRGFYDAYRAEYPLDAGYETRKQLYNLYHVLNHLNLFGG
                     GYRAQAERIIERLLAEVRL"
     gene            complement(15366..16760)
                     /gene="argH"
                     /locus_tag="AC16CX_03580"
     CDS             complement(15366..16760)
                     /gene="argH"
                     /locus_tag="AC16CX_03580"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3011626.1"
                     /translation="MPDNDKIRNQPWSGRFGEPAAELTQRFTASVSFDRRLAEFDIEA
                     SIAHARMLHATGIIAKNDLSAIEKGLAQIRSEIRAGGFPWSEADEDVHFNIERRLTEL
                     VGEPGKRLHTARSRNDQVATDVRLFLRAAIDQAAATIKSVQRALIDLADEHAETVMPG
                     YTHLQVAQPVSFGHHLLAYFEMLARDAQRLADCRRRLNRLPLGAAALAGTSFPIDRLM
                     VAKELGFDGVCENSIDAVSDRDFAIEFCACAALVMTHLSRFCEELILWMNPRFGFVEL
                     ADRFRTGSSIMPQKKNPDVAELIRGKTGRVNGHLVALLTLMKAQPLAYNRDNQEDKEP
                     LFDTVDTLTMSLAVFAEMLPAIKVNREAMEAAARQGHATATDLAEYLVRKGVPFREAH
                     EAVAQAVRFAESRGCELSELKISELQRFSPAIGKDALAALTLEGSLKSRTHLGGTAPV
                     RVKAAIAKARKSLG"
     gene            16926..17945
                     /locus_tag="AC16CX_03585"
     CDS             16926..17945
                     /locus_tag="AC16CX_03585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF018305.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3011627.1"
                     /translation="MARSINQNAYTGALPNFRNLGILLRILLIVNVFAFAAAVVKSVD
                     ARALWQQVVEISGLVEPLLILSLLALAALNHLLRRMPYWLGAMAVLALELALTTALYI
                     AGRPMLGAEPGPLERYWLLVAFTTAVLLVYFDLRGRALSPALSEARLQALQARIRPHF
                     LFNGINAVLSLIRQDPRRAEAALEDMAELFRVLMADNRELAPLSREVELCRQYLDLEQ
                     LRLGERLKVEWRTDRMPRDAVVPPLVLQPLLENAVYHGIEPRVEPGVVSIDIYAVGDR
                     VHAVLRNPYQQQDDHHAGNKMALANIRERLQLHFDAEATLTAKVADDTFQVHIVMPYI
                     KGRES"
     gene            17963..18715
                     /locus_tag="AC16CX_03590"
     CDS             17963..18715
                     /locus_tag="AC16CX_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3011628.1"
                     /translation="MSEAKPLRVVIVDDEAPARSRMRDLLGECADEVPLTIAGEAASG
                     RAALELLSRESADVVLLDIRMPEMDGIEVAQHLQRLERAPAVVFATAYDAYAIRAFEV
                     HAVDYLLKPIRLARLKEALVRAARPRPEALRGLARAPRTHLSAQERGRIHLIPVADVI
                     YLKAELKYVSVRTAAREYLIEESLTRLEEEFGERFARVHRNCLVARAAVRGFERAGEG
                     GEAHWEVLLNGVDERIAVSRRQQHMVKEFSRG"
     gene            18827..19777
                     /gene="hemC"
                     /locus_tag="AC16CX_03595"
     CDS             18827..19777
                     /gene="hemC"
                     /locus_tag="AC16CX_03595"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR3011629.1"
                     /translation="MTTTASGKEAPQRIVIASRESALAMWQARHVQARLAALYPGTGI
                     DIVGVTTEGDRRLAASLAKIGGKGLFIKELEETLAAGRADIAVHSVKDVPMTLTSGFV
                     LAAIGERADPRDAFVSNRHADLSALPAGARVGTSSLRRECQLRARHPRLRIEPLRGNV
                     TTRLRKLDEGRYEAVILAAAGLKRLGLEDRITRLLTPSESLPAPGQGALGVECLENRT
                     GLIELLAPLEHAATARCVAAERAFSRTLAGSCNVPLGAYAEVENGKIRLRGFVGAPDG
                     SRLVSGELAGAADQPEVLGTALAEQLKAQGAAEILAQLQT"
     gene            19955..20734
                     /locus_tag="AC16CX_03600"
     CDS             19955..20734
                     /locus_tag="AC16CX_03600"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3011630.1"
                     /translation="MKEQSLRGKNIVVTRPANQARELARLIEAAGGTPILFPVIEIRE
                     LEDPRPFLALVDRLDEFDFAIFISPNAVGRAMKLILRRRTLPRALKVIAVGGGTVRAL
                     RGYGVSEVIAPRDRYDSEALLEMAELVDVDGKRVMIFRGEGGRELLGDTLKARGASVE
                     YAECYRRGKPDLDPAPLLGAWARNELDAVTVTSSDGLRNLFEMVGTSGRERLVITPLF
                     APHPRIAETARALGVRKVIVTGPGDEGLLTGLTDYFKKRGS"
     gene            20915..21988
                     /locus_tag="AC16CX_03605"
     CDS             20915..21988
                     /locus_tag="AC16CX_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016275.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3011631.1"
                     /translation="MNDSSQPDRPPAAAGSTAGGGAAERAPALHRSLPVLLVAALALG
                     LAAWTWYDSRSEIGALRQELAKKLADADTQNKESRLVAEQVREAVAGAQVKLGVLESR
                     LAESQNQQIALEALYQELSRNRDEWAYAEIEQSLFIAGQQLQLAGNVKVALIALQTAD
                     ARLQRMNRPQLTALRKAINRDIERLKSAPHVDTVAISVRLDNLIAQVDKLPLAMEVRP
                     QPEQPAAAQKGAEDSPWTRFWRDAWAELRQLVRVQQMDQPDVPLLAPAQSYFLRENLK
                     LRLIGARLALLARDAASYKADLKAARDWLGRYYDTRNNSVAYAQGMLRNLHDAEVSIE
                     TPDISATLEAMRGVRLSRERGAR"
     gene            21985..23193
                     /locus_tag="AC16CX_03610"
     CDS             21985..23193
                     /locus_tag="AC16CX_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HIR3011632.1"
                     /translation="MKTLIWVVAIIVLAVAITLAARYNTGYLLLVLPPYRVELSLNLL
                     VVLAVTMFAVGYALVRFMAATLRLPRQVREYRLARRKEKAQATLTEALREYLAGRYGR
                     AETVAAASLELGEQPGLAALLAARAAHELRAYERRDAYLDRAASAASGGEAARIVTTA
                     DLLLDERRYQEALDLLKLLPRRHTAALRLELKAQQQARNWEQVFALAEELRRRNVFDA
                     TQAGEVRRYALAENLKRKALDSRALEEAWEKVPSSDRKDRRVAAAAAQCFIALGGCDK
                     AHRIIEEALEAGWDSDLVGLYAECEGGDTVRRIERAETWLKIQPGDAALLLTLGRLCA
                     DQGLWGKAQSYLEASLAIEPTHSAHLAAARLQEQLGNAEAARRHYRESLDLALAQLKT
                     VTGGRRRSQL"
     gene            complement(23267..24166)
                     /locus_tag="AC16CX_03615"
     CDS             complement(23267..24166)
                     /locus_tag="AC16CX_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3011633.1"
                     /translation="MSHPGARKFALLSIAAAVATIGLKTLAWWVTGSVGLLSDALEGT
                     INLAGATVVLAMLGIAARPPDEDHAFGYTKAEYFASGLEGTLILIAAIAISVAAVDRL
                     IAPRPLEHVGPGLAVSVAAALVNYAVARRLFEGGRDYRSIALEADARHLMTDVWTSAG
                     VIVGVAAVWVTGWERLDPLIALAVAANIVWSGYELMQRSIKGLLDRALPPEQLRILDR
                     VLDRYRARGVDFHALRTRQAGARSFVTLHVLVPADWTVAQGHDLAHQVEEDIRKELPD
                     AVVLTHVEPIGYPESYRDIDFDR"
     gene            complement(24292..25311)
                     /locus_tag="AC16CX_03620"
     CDS             complement(24292..25311)
                     /locus_tag="AC16CX_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HIR3011634.1"
                     /translation="MPHKVTIQSSGHAFSVSDGETVLAAALREGIVIAYGCRHGSCGT
                     CKGKLLEGSVDYGTYQEQAMSEAEKRAGMALFCQAKPHSDLLIQCREIGAAVKGIQIK
                     MLPTRVMKMERAAPDVMLLELKLPANEKLRFLAGQYVDLLLRDGTRRSFSMANPPHDE
                     GFLQLHLRNYGGPFSRHAFGTMKERDILRLEGPFGTFFLREESAKPIVLVASGTGFAP
                     IKAIIEHALHTGVKRPMTLYWGCRVRADLYLHGLPERWQREGRLRYVPVLSEALPTDN
                     WTGRTGFVHRAVTEDFPDLSGYQVYACGAPVMVEAAHKDFTSRCGLPEEEFFSDSFTP
                     AAFGN"
     gene            25476..25805
                     /gene="cutA"
                     /locus_tag="AC16CX_03625"
     CDS             25476..25805
                     /gene="cutA"
                     /locus_tag="AC16CX_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR3011635.1"
                     /translation="MDEVVVLLTNLPDRDTALKLARELVAKKLAACVSVLAECTSVYR
                     WKGELENAVEVPVLIKTRAARYDEVEAAIRALHPYELPEILAVPAVRGLDEYLQWVAD
                     ETAIRES"
     gene            25886..27667
                     /gene="dsbD"
                     /locus_tag="AC16CX_03630"
     CDS             25886..27667
                     /gene="dsbD"
                     /locus_tag="AC16CX_03630"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     [NAD(P)H] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HIR3011636.1"
                     /translation="MRLVLIFLLTFSCLARGAEPELLEPEKAFRFSARLKDERSIEVR
                     YQIAPGYYLYRDKFRFALAPEDAKLGAVQLPAGKKHRDDFFGEVETYRGDLRITLPFE
                     ADRDGVPAVTLTAISQGCADIGVCYVPQEQKAVLRPAALGAAGTRSDASSTPFGAAPA
                     AGEDARLAALFDGGFWLAVASFFGFGLLLSFTPCVLPMVPILSGVIVGAGHSITRVRG
                     FLLAAIYVFGMAITYAVAGVAAGLSGEMLSATLQNPWVLGAFAAVFVALALAMFGFYE
                     LQLPVALQSRLADASNRFHGGHFAGVFSMGVLSALIVGPCVAAPLAGALLYISQSRDV
                     VLGGAALFAMALGMGVPLLVVGASAGALLPKAGPWMETMKRFFGTLLLGVAIYLVSPF
                     VPLPVQMFLWAALLIVTGIYLRAIDPLPVNAHGFQKFSKGVGVIALIAGVAYLIGAFS
                     GGRDLLQPLSGLRLTASESGPHETPFQRVSNAAELDARVAAAAGRPVMLDFYADWCVS
                     CKEMERYTFSDERVRARFREMVLLQADVTANNAEHAALLKRFRLFGPPGIVFFDREGR
                     EIRGLRVIGFQPADRFAAVLDQALNFR"
     gene            27664..28197
                     /locus_tag="AC16CX_03635"
     CDS             27664..28197
                     /locus_tag="AC16CX_03635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3011637.1"
                     /translation="MRKARALALFAAVAAAAAVVGGAAYHLWLRGGAPAPQAIVERVY
                     AARLADLKGGAQTLAQWRGQVMVVNFWATWCAPCREEIPGFVRMQERYGSRGLLFVGI
                     ALDQPDKVAEFAREFRINYPLLIGGLDTLELLRQAGNRAGVLPYTLVIDRRGNLVSRE
                     PGGLKEARLEGIILPLL"
     gene            28502..28954
                     /gene="aroQ"
                     /locus_tag="AC16CX_03640"
     CDS             28502..28954
                     /gene="aroQ"
                     /locus_tag="AC16CX_03640"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3011638.1"
                     /translation="MAGIKHILVINGPNLNLLGSRETEHYGRDTLKAINARLAQRARK
                     AKVRLATYQSNSEAALVARVQQARREKVDFIIINPAAYTHTSVALRDALAAVGIPFVE
                     VHLSNVFARESFRRESYFSDIAVGILSGLGAKGYELALEFALQYQKKG"
     gene            28957..>29321
                     /gene="accB"
                     /locus_tag="AC16CX_03645"
     CDS             28957..>29321
                     /gene="accB"
                     /locus_tag="AC16CX_03645"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3011639.1"
                     /translation="MDLRKLKTLIDLVQQSGIAELEITEGEEKVRICRGVVGGQPAAP
                     ATQVVMLPQAAGMAPAVAGGAPAAPAESEAAAAPEGHVIRSPMVGTFYRSSAPGAKPF
                     VEVGQAVKAGDTLCIIEAM"
BASE COUNT         4918 a         9568 c         9930 g         4905 t
ORIGIN      
        1 ctgcttgggg tgcagcagga cgtcatcgtc cggctgcgcg ttcaagtcga gcgcgaagtc
       61 ctcgccgccg atattcatcg ccaccacgcg cgggctcgcg cgcgggatct cgtcgatgcg
      121 ggtgaacgcg tccgcggtct cgatcatcac gatgaaccgg gtgtgcccgg ccttcatccc
      181 gcgcttttcc tcgagtccgc tgacgagctc gtcgagcagt ttcacgtgcg aggcgctgtc
      241 aaccttggtg atggcgatac cgtcgacatc ggggccgacc gagtgctcga gatcgcgcac
      301 cgcgagggac agcggctggt tgatgcgcac caccacgtcc gcgcccccgc gcctcacgcg
      361 cgccgcgttt ctcgcgacca gcgtgcgtgc tttctccttc tccgcggggg gcaccgcgtc
      421 ctcgaggtcg agctggatca cgtccgcgcc gcgggtgtgc gccttctcga tgaatttctc
      481 gacgttcacc ggcacgtaga gtagcgaccg ccacaccggc agatcgggtt tcattgtcat
      541 tccttggtca gcgataagcg gtgagcggaa aaatagcgac tggaatttta ccgcttactg
      601 ctcaccgctc acggccggcg ggtttccgcc ctcgcatgcc acgccggtgg cgagcagctg
      661 cgagtaggcc gcgtcgtcca cgccgatctc ggcaagcagc tgccggttgt gctcccccag
      721 ccgcggggcg ggcgtgcgga tcgcgcccgg ggtgcccgac aggcgcggga ccacgtgatg
      781 catcggaaac gcgcccatat cggcgtcagg atagtccgcg acgagctcgc gggcgatcac
      841 gtgcgggtcc tcgagtatct gccggatatc gtagatcggg ccgatcgtca cttcggcctg
      901 ctcgaagaat gccacgttct cggcttgcgt gcgttgcgcg acgaattcca cgatgattac
      961 gtcgagatcg tcggcgtgct tcaccctctc ggcgttggtg cggtagcgcg gatcgtcgat
     1021 gagatccggc cggccgatcg agcggaacag ccgctcggcc atcttctgcg tggaggacga
     1081 caggcagacg tacaggccgt ccttgcagcg gtaggcgttg cgcgggccgg cattggtcga
     1141 gcggctgccg gtgcgcggtt tgacctttcc cgtcaggcgg tagttggcag cctggggccc
     1201 gagcacggcg aacagcggat cgagcagcgg caggtcgatg acctggccgc gccctccgtt
     1261 ttgctcgacc tcacgcagcg cgatcatcgc ggccgccgcg ccgtagagcc cggcgaccgt
     1321 gtcggcgagg tacatcggcg gcaacaccgg ctcgcggtcg gcgaagccgt tgaacgaggc
     1381 gaaacccgac atcccctcga ccagcgtgcc gaacccgggg cgccgctggt agggcccgtc
     1441 ctggccccag ccggaaatgc gcacgatgac gagcttgggg ttgaacccga gcagccgatc
     1501 ggggccgaag cccatcttct cgagtgtccc gggacgaaag ctctcgacca atatggctgc
     1561 ggaagggaga aggcgcgcaa gcaagtcgcg cgcctccggc ttgcgcaact ccaggcccag
     1621 gctcttcttg ttgcgcgcat agagcttcca gttcgtctgc acgccgctgg tctgccacgc
     1681 gcgcagggtg tcgcccgccg gcggctcgat cttgatcacc tcggcgccga aatcgccgag
     1741 tacctgcgtg agcgtgttgc ccgcgacgag acgggagaga tcgagcacgc gcacgccggt
     1801 cagcggcccg gtggccgcgg gatcgtagga tttgcggggc aacgccgcgg tcattcgggc
     1861 ctgatgctgg cttcgagagc gaccttgcgc cagcgcgtga tttcgttggc gacgaaccgg
     1921 gagaactgtt cgcgcgtcat ggggctgggc tccgcgccct ccgcggcgaa gcgctgcttc
     1981 aagtcggccg actgcagtgc cttggccacc tccgaggcga gacggttgag gacgtcattc
     2041 ggcgttttgg acggtgcggc aatcccccac cagaactcgg cgaagtagcc gggcacgccg
     2101 gactcgctga tcgtcggcag ctcgggcatg aaggacgagc gctgttcgct gccgacggcg
     2161 agcgccttca gccggccggc ctgcacctgg gtcatcgccg agggcaggct cgtcatcacg
     2221 agctgggtat ggccacccat gaggtcggtc agggccggcg ccaccccctt gtacggcacg
     2281 tgcacgatct tgacgccggc catccgcgcg aagagctcgg tggccaggtg attatgaccg
     2341 ccggtgccgg tggaaccgta gttgatctgc ccgggctttg cgcgtgccag cgcgatcagc
     2401 tccttcacgt tcttcaccgg cagcgaaggg tgcacgaccg cgagcagcgg gcctcgtgcc
     2461 atcatgccga tgtaggcgaa atcgcccagt ggatcgtagg ccatcttcgc gcgcagcgcg
     2521 gcgttggccg tgaacgacgc ctgcacgttg acgagggtgt agccgtcggg caccgacttg
     2581 gcgacgtgat ccgcgccaat catgccgccg gcgccgctgc gattgtcgat gacgaccggc
     2641 tggccgagcc ccgggctcat cgcctgcgca gtgatccggg agagcacatc gttgctgccc
     2701 ccaggcgggt agggcacgac caggcgtatc ggcttggcag gatacggctg tgccggggcg
     2761 gcgcctgcaa acgccagcgc gccaagcgca aggcaggcgg cccgtatcac gctcaacgat
     2821 gctcggcgcc gctcgcgagc tgacgctcga tctggcgcga ctcgcccttg agcagctggg
     2881 cgacttccgt cgcgcgcttg tcgctcatgc ggctggaaat ggtggacacc gacaacgcgg
     2941 cgaacgcgat gccgctctgg ttgcgcagcg cctgagcgac ggagcgcacg ccggcgagcc
     3001 ccggcacatc gcgcatggcg taacccagct tctgcgagcg cttgacttgc gcgaagaggg
     3061 cgcccgtgtt gaggccatac tcgggcaggc gcccggcgtt ggcgccgacg accttctgga
     3121 tttcctcctc cggcagcgcg gaaaggatgg cgagtgagcc ggtgcccacg cccagcgggc
     3181 ggcgcatgcc gatctcgagc gtgaaggtct tgatcgggaa cgtgccttcg cgccggtcga
     3241 ggcacaccga gtcgagcccg ctccgctggg tgaggaatac cgtgtcgccg gtcgcttcgg
     3301 cgatgcgcgt caacgccggg tggcagatct cgcgcaggtt gaagcgcggc gtggcggtga
     3361 gccccagctc gaacaccatc ggcccgagaa aatagcggtg cgtgtcggga tcctgctgca
     3421 ccatgttttc cgtggcgagg cacttcaaca tgcgatgcac ggtcggtcgc tgcagcccgg
     3481 tgcgcgtggc gaggtcgagc aggcgcgagc cgccgcggtt gtgcgcggcg atttcgcgca
     3541 acagcatgag cgagcgctcg atgctctggg tgccggtcag gcggttgccg gccccggctt
     3601 cggtcttgcg tggcatagat ggttgagtcc gtatcgtgag cgaaccgcga ttatgccacc
     3661 agagtggttc tggcaaaacg gggtatgttg tgctaactta aaacccacat cctggacgcc
     3721 aaggattgga tcacatgatg gacttcaggg ccggcctgtg atcccggttc ccgtcccttc
     3781 cgagcgcagg tcatgaacga acgcacctct tccactgccc cagccggctc tgccggcgcg
     3841 cgcttccgcg cggcgctcgc cgaggagcgg ccgttgcagg tgatcgggac catcaacgcc
     3901 aaccacgcgc gcatggccga gcgcgtcggc taccgcgcga tctacctctc gggcggggga
     3961 gtggccgccg gctcgctcgg ggtgcccgac ctcggcatca gcggcctcga cgacgtgctg
     4021 accgacgtgc gccgcatcac cgacgtgtgc ccgctgccgc tgctggtgga cgtggacacc
     4081 ggcttcgggg cgagcgcctt caacatcgcg cgcacggtga aatcgctcat caagttcggc
     4141 gccgcggcga tgcacatcga ggaccaggtc ggcgccaaac gctgcggcca ccgccccaac
     4201 aaggagctgg tatcgcagca ggagatggtg gaccgcatca aggccgcggt ggacgccaag
     4261 accgatcccg cgttcgtggt gatggcgcgc acggacgcgc tcgcggtgga gggcctgcag
     4321 gcggggatcg accgcgcctg cgcctgcgtg gaagcgggcg ccgacatgat ctttcccgag
     4381 gccatcaccg agcttgcgat gtaccggaaa ttcgccgacg cggtgaaagt gccgatactc
     4441 gccaacatca ccgagttcgg acagacgccc ctctacacgg tgaaggagct gggcacggcg
     4501 ggcgtggcca tggtgctcta tccgctctcg gcgttccgcg ccatgaacgc ggcggcgctg
     4561 cgcgtctacc aggcgatccg caaggacggc acgcagaagg gggtcctcga gctgatgcag
     4621 acgcgcgagg agctctacga cttcatcggc taccacgcct acgagcgcaa gctcgacgag
     4681 ctgttcaccc gcgacaagaa gaacaagtga tgggtgatga gtgatgggtg atgggtaaac
     4741 ccgtcaccgg tcacctgtca cggtatttca atgaccggga tactgctgtc cgacggcacg
     4801 cgcgcgagtt accgcgacga gctttgacgc cgccgcccac ggcgcgctgc aggcagcgcc
     4861 ggccggcggt ataggctcta taataatcac cctcttttca cggccctgcg cccgatgcag
     4921 ctcttcgcct tcggcatcaa ccaccagacc gctccgctct cggtgcgcga gcaggtggtg
     4981 tttcacgccg agaacctggt cggagcgctg cgcgagctgg tggaccatcg cccggtaaag
     5041 gaggcggcga tcatctctac ctgcaaccgc accgaggtct attgcaacac cgaggacccg
     5101 cgcgcggcgg tggactggat ggccggctat cacggcttca agccccagca gatcgagcag
     5161 tatctctaca agctgccgca ggaacgcgcg gtcaagcacg ccttccgtgt ggcgagcggg
     5221 ctcgattcga tggtgctggg cgagccccag atcctcggcc agttcaagca ggcggtgcgg
     5281 accgcccagg cggcgggcac gctgggcctg ctcctgaaca agctgttcca gcgcaccttc
     5341 gcggtggcga agacggtccg ctccgagacc gagatcggcg ccgccacggt gtcgatggcc
     5401 tccgccgccg tgggcctcgc gcagcgcatc tatccctcga tcgtcggcca gaacgtgctg
     5461 ttcatcggcg cgggcgagat gatcgagctc accgccaccc atttcgccgc ccagcatccg
     5521 aagcgcctga ctttcgccaa ccgcacgctc gagcgcgcgc aagtcctcgc cgaccgcttc
     5581 ctcggccgcg ccatcacgct gaacgagctg ccctcgcagc tcgcttccca cgacatcata
     5641 atcagctgca cggcgagcac ccttcccatc atcggcaagg ggctggtgga gagcgcgctg
     5701 cgcgcgcgca agcatcgccc gatgctgatg tacgacctcg ccgtgccgcg cgacatcgag
     5761 ggggaggtgg gggcgctgga tgacgtcttc ctctacaccg tggacgacct cggcaagatc
     5821 gcgcgcgagg gcttggacgt gcgcagcaac gcggtggcgc aggccgaggt gatcatcgag
     5881 aaccaggtca ccgacttcat gcactggctc ggcaaccggg agctggtgcc gacgatacgc
     5941 gcgctgcgcg acaacgccga acgggcgcgg cggcacgaga tggagcgcgc gctgcgacgc
     6001 ctggcgaaag gcgaggaccc gaaggcggta ttggagcagc tctcgcacgc gctcgccgcg
     6061 aagctcacgc acgcgcccac ccacgcgctc tcgcacgccc gcgacgagga ccgcgaccag
     6121 ctggcgcagc tgctgacccg cctctaccag atcccgcggc cggaataaga gcattgcgcg
     6181 caggcacttg ttctccgcgc ttctgcagat gaaaccaagt attgcagcga aattgtcgca
     6241 gctctcggga cggctggagg aaatcaaccg gctgctcgct ctggagaacg ccaccgccga
     6301 tctcgaccaa taccgcaagc ttacccgcga gcacgccgag atcgcgccgg tggtcgagct
     6361 ctaccagcag tacgtgaagg gcgaggccga catccaggcg gcgcaggaga tgagcgggga
     6421 acccgagatg cgcgagttcg ccgagtccga gatcaggcag acccgggagc ggctcgccgg
     6481 catcgagcag gagttgcaga agaagctgct gccgagggac cccaacgacg agcgcaacat
     6541 cttcctcgag gtccgcgccg gcaccggggg cgacgaatcc gcgttgttcg cggcggagct
     6601 gttccggatg tatagccgct acgccgagcg caaccgctgg caggtggagg tgatctcgca
     6661 aagcccctcg gacctcggcg gctacaagga aatcatcgcc aagatctcgg ggcagggcgc
     6721 gtactcgaga ctcaagttcg aatccggcgg gcaccgcgtg cagcgcgtgc cggtgaccga
     6781 gacccagggc cgcatccaca cctccgcctg caccgtggcg gtgatgccgg aagccgacga
     6841 gatcgccgat gtggtgctca atcccgccga gctgcgcgtg gacaccttcc gcgcctcggg
     6901 ggcgggcggc cagcacgtca acaagaccga ctccgcggtg cgcgtcacgc acctgcccag
     6961 cggcatcgtg gtggaatgcc aggatgaccg ctcccagcac aagaaccggg cgcgcgcgct
     7021 tgccatcctc gccgcccgca tcaaggacag gcagatccgc gagcagcagg ccaggctcgc
     7081 cgcaacccgc aagtccctca tcggcagcgg cgaccgctcc gagcgcatcc gcacctacaa
     7141 tttcccgcag gggcgggtga ccgaccaccg catcaacctc accctctaca agatcggcca
     7201 ggtcatggac ggcgagcttg acgagctgat ctcggcgctc gcggccgagc accaagccga
     7261 gcagctcgcc cagctcgccg aagaaacgca tccggtgtaa gccattccaa gcgtggcatt
     7321 tcctgccgag gtttccctgc aagccgtgac ctttgccgtc ctgtcggcgc tcggtttcgg
     7381 cgcggcgtta gtaaccactc actcaggcct gaagcacgtg gatgccacga ccggatccaa
     7441 ggtaagcatc cccacggcta cgttgctctt ttggttgctc tcgccgttcc tggactggac
     7501 cggctggcag accgcggcga tcggcatatt cgccctggtg ggtttgttct ttccagcggc
     7561 cgtgaccctg ctcaccttcg aggccaaccg gcgcctcggc ccgactgtca cgggaaccat
     7621 cggcagcacc gcgccgctgt ttgcggtgct gggggcggcg gcgtttctgg gggagccgct
     7681 cggcgtccgc gcgctcgtcg ccactgcgct catcgtgctc ggatcgatcg cgctgtcgag
     7741 gccgcaagtc gcggacacgg agacgcgtca gagcggcacg ctgtggctgc cgtggtcagc
     7801 ggccgcgctg cgtgcactgg cccaggtgct gtccaaggcg gggcttgccc tctggcccaa
     7861 tccgttcgcg gccgcgctgg tgggctacac cgtgtcatcc gcggtggtgt gggcggccgc
     7921 gttcgaccgt cgcgcacctg cgccggtgtt caaccggcac gggatcgcct ggtttgccgt
     7981 gaccggcgcc ttgaacggcg cagcggtgct tttcctgtac tgcgcgctca acaatggccc
     8041 cgtgtacgtc gtttccccga ttgtcgcgac ctatccgttg ttcaccatga tgctgagcgt
     8101 gctcgctttg cgcgaggagc gcttgaacgg cgcgttgatc ggcggcgtgg cgctcaccgt
     8161 gacaggcgtc gtcatattgt tggcgagatg gtaaaggcgc gcagtatggc gggggcgagc
     8221 atcgatgaga tgttgcgatc ggcgggtatc gatcctgttg atgcgcgcgt attgatgcgt
     8281 gcggcgctgg gagtcagcga cgcgcatctc gccgcgcatc ccgaacaggc gctgagccgt
     8341 gatgaacgcg accgctatct tggatgggtc gagcgccggc acgccggcgc gccggtcgcc
     8401 tacctcaccg gcgagcgcga gttctacagt tttgctttca aggtgacgcc cgcggtgctg
     8461 atcccgcggc cggaaacgga actcctggtt gagaccgcgc tcgagcatat tcccgtggac
     8521 gagccgtgcc gcgtgctcga tctcgccact ggcagcggct gcgtctcggt ggcgatcgcg
     8581 aaacaccgtc cccgcgcgcg ggtgacggca acggagactt cgcgcgaagc gctcgcgctg
     8641 gcacgggaaa atgcagcgcg gcacggcgca gccgtcgaat tcgtcgaaag cggctggttc
     8701 gaagcgcttt caggcaggcg cttcgaaatg atcgttgcca atccaccgta catcgccgcc
     8761 ggcgatcctc acctcaacgc aggcgatgtg cgtttcgaac ctcgcgacgc gctggttgcc
     8821 gggccgaccg ggctcgaatg catcgaggtt atcgtggagg gggcggcgcg ttacctgacc
     8881 gcgggcgggg ggtggctcct gttcgagcat ggctacgatc agggggcgtg ctcgcgcgcg
     8941 ctgctcgctg cggcgggata cgaggacgtc gtcacgcgcc gcgacctcgc cggtatcgag
     9001 cgggtgtccg gcggcaaggt ttgacggggg aatggctgaa gcgttaaact aatgtccgat
     9061 taaattactc gggtatatgg agaaatcatg agtattcaag atgttataaa gaaccaagtg
     9121 agtaaaaacc gggtcgttct gtacatgaaa ggcacacccg atttccccca gtgcggcttt
     9181 tccgccaatg cggtcggaat cctgcatgcc tgtggggtgg atgagttttt ctcggtcaac
     9241 gtgctggaga acccggacat ccgccagggc atcaaggagt acgccaactg gccgaccatt
     9301 ccgcagctct acgtgaacgg cgagttcgtc ggcggctccg acatcatgac cgagatgtac
     9361 aagagcggcg agttgaagaa gctgctcgaa aaaaccgctg cttgagcagc ggttttaccc
     9421 cctcatcaga aaacggccgg cctcaagccg gccgtttgct tttccagaat ccgccatatg
     9481 gcgggtgacc catcactcat cacccatcac tcatcacgcc ctcaagcgaa tagcgaccag
     9541 gcgttctgcc cgagcagcac cagcgcgatc acaaacaggg cgcgcttgac ccagatgcgg
     9601 aatgcccggg cgtcgatgcg gttgcgtatg cgcaccccgg tgagcgatcc caccaccccg
     9661 atgaccacca gcggcagtgt ggcgaaccac tgctccgctg taacgccgcc gccggcggtc
     9721 aacaccgtga actgcgttgt cttgcccacg aggaagcaga tgttgagcgc ctgcaccagc
     9781 atgacggggg gcaggtgcaa cgcgagataa aagatgatga gcggcggggc ggcgatattc
     9841 gccgtgcctt cgaagatgcc cgccgcgagg cccgacagcg gcgcgaacgc gcgctcatgc
     9901 cgccgcacga gcggccactc ggcccgccct aggcggtcga gattgagata ggcgagcgtc
     9961 atcagcgcga gcagcagcgt gtacggcacg tccggcgcgg atacgaacag ccacgtaccg
    10021 gcggcggacc ccgctacggc gtagagcggc atcatccaga aacgcgcgag cgtcgcgcgg
    10081 atcggaccgc tggtgacgag cgcgatagtg atcgtcgcga gcgtcggcag cagcacggcg
    10141 atcaccgcgg tgcgaatgtc ggtgaccatc gcgatcagcg gggttgcgat gaacggaaag
    10201 ccgaagccta acgcgccctg gctgaggccg gcgaccagga cgacgcacgc catccaggcg
    10261 atcagtcccg cagaaaagcc ttcaagtggc atggcttgcg cgtagaaacg ccccaccgcc
    10321 gaaggcggtg gcgctcggcg gcatcaggcg ggtatcggtg catggcgcca ggcatgctca
    10381 tggggcgcgg tgaaagagca acatcatgcg gagttcgcag tttatcaaag gcatgcggtt
    10441 gctccggcgg tgggagcggg tgccggatcc gctcattcaa ttgtcatgac gccgcaattg
    10501 cactaggatg gtctgcgacc gaccaccatt tctggaggtt tgccatgagc cagcccaacc
    10561 ccttcctgtc ctccggttct aacccttcct cgagtgccgg tcccgcggac ggcgggcgcg
    10621 ccgtcgacgc cggccagggc tgggcgtgga tcgcgggcgg cttcgacctg ttcaagcgca
    10681 aagccggcat ctggatcgcg ctgatcatcg tgcttgccgt gatcctgatc gtgatgagct
    10741 tcataccctt cctgggccag cttgccatgt ttctcctgtt gccggttttc accggcggac
    10801 tcatgctggg ttgccaggcg ctcgagcgcg acggatcgct cgagatcggc cacctgttcg
    10861 cgggattcaa ggcgcacacg gggagcctga tcgtgctcgg cgtgttcggt ctggtggcta
    10921 cgatcgtcat catgcttccg gtgatcgcga tcgtgggcgg cggcgcgttc tttggcgcca
    10981 tgcgggggga tgccgcgggc atcggcgcga tcggcgcgag tttcgcgctc gcatttctga
    11041 tcgcattcgg tctcagcatc ctcgtctaca tggcgctgtg gttcgcgccg gcgctggtgg
    11101 tgctgcgcgg ccttgcgccg atggaagcgg tcaagcagag ctttctcggc tgcctgaaga
    11161 acatcattcc gtttctgatc tacggcatca tcatgctggt gcttagcgtc atcgcgtcga
    11221 ttccgctggc gctcgggtgg ttgatcctgg gtccggtcct cgtcgcttcg gtatacgtcg
    11281 cttaccgcga tatctacccc gctagctgaa cgttgaaggc gtgcaactaa actcgccggc
    11341 cggttatccc tgacgcccgc caccggcagg ggtcatatct cagttcctat agaataacag
    11401 taaatcgttg ttattcaatt agttgatata atatatgaaa gcgcttgcat aactgatcgc
    11461 gggtgtataa tggccgctca ggcggtcccg cacgattgcg ccattttctg gagggagtcc
    11521 ccatgaacct catgacggaa gcgaaaaacc tcaccacgtc gaacaaaccc gcaagcggca
    11581 aagtgcgcat gagcccgtcg gaggcgttca tcgagacgct cgccgcgcac aaggtgaagg
    11641 acgtgttcgg catcgtcggt tcggcctata tggacgcgct cgatctcttt cccggcgccg
    11701 gcatccgcct ctacagcgtg gcgcacgagc agaacgccgc gcacatggcc gacggctacg
    11761 gacgcgtcac caaccggccg tcggtatgca tcgcgcagaa cgggcccggc atcaccaatt
    11821 tcgtgaccgc ggtggcggcg gcgtactggg cgcacacgcc gatggtggtg gtgacgcccg
    11881 aatcgggaag cctcacggcg ggccacggcg gcttccagga aacggagcag atgccgatct
    11941 tctcgaaaat caccaagtgg caggtgcacg tgaattcgcc cctgcgcatg gcggaactga
    12001 tgcaccgggc gttcaccctc gcgctgcacg agcgcgggcc ggtgcaggtc aatattcccc
    12061 gcgattactt ctacggggag ggcgactacg agatcctgcc gccgctcgtc atcgagcgcg
    12121 ccgcgggcgg ctcgaattcc ctcgaccggg cggtgaagat gctggccgag gcgaaattcc
    12181 cggtcatcat gtcgggcggc ggcgtcgtga tgtcggacgg ggtcgatgag gtgaaggcgc
    12241 tcgccgagca cctcaccgcg ccggtggtga acacctatct ccacaacgat tcgttcccgg
    12301 cggctcatcc gttgtcctgc ggcccgctcg gctaccaggg ctcgaaggcg gcgatgcgca
    12361 tcatctcgcg cgccgatgtg gtgctcgcgc tcggcacgcg gctcgggccg ttcggcacgc
    12421 tgccgcagca cgggatcgat tactggccaa aggacgcgaa gatcatccag atcgacgccg
    12481 accatcgcac gctggggctg gtgaagaagg cctcgctcgc gatccagggc gacgccaggc
    12541 tcgccgccgc ggaactcctg aagcggctga aggcgcgcgg cgcgcgcaag cccgacaagg
    12601 cgcgcctcgt cgaagtacaa agggaaaagg aagcgtgggc cgccgagctc gcgaactggc
    12661 catcgcccaa caagaagggg cagatcggtc cgcgccgcgc gctcgcccag ctcgcgaagg
    12721 cgttgccgcg gaacgccatg gtttccaccg acatcggcaa cgtgtgctcg gtcgcgaaca
    12781 gctacctgcg cttcgagcag ccgctgtcct tcctcgcggc gatgagctgg ggcaactgcg
    12841 gctacgcgta tcccacggcg ctcggcgcca gggtcgcgcg tcccgaccgg ccggcgatcg
    12901 cctacgtcgg cgacggcgcc tggggcatga gcctcgccga ggtgatgacg gcggtcaagg
    12961 agaagatccc ggcggtggcg gtggtgttca acaatggcca gtggggcgcg gagaagaaga
    13021 accagatcga ctacttcgac aaccgcttcg tcggcaccaa cctgacgaat ccggatttct
    13081 cggaggtcgc gcgcgtgatg ggggccgagg gctaccgcgt ggagcacgag gacgaggttt
    13141 ccgacgcgct cagggcggcg ctcaagtccg gcaagcccgc ggtggtcgag atcatgctca
    13201 cgcaggagct cggcgagcca ttccgccgcg atgccttccg caagccgcgg cgactgctgc
    13261 ccaagtatcg ccgctacagc gccaaggaat accggtgata gtggcttgat cggatgacga
    13321 aaaaaagccg gccccgcgcc ggcttttttg gttagtgggt gctaactggg ggggcagtcg
    13381 gagcgtgaat cagggtccgg gcagcaagag cgccaagctg agcgacgtgg cgcggctcgc
    13441 gcgggtttcc accgccaccg tctcgcgcgc gctgacccag cccgacaagg tcaagccggc
    13501 gaccgccgcg cgcatccggc aggcggtgca ggcgctgggc tacgtagccc acggcgcggc
    13561 gcgcgcgctc gcctcgcgcc gcacgcatac catcggcgcc gtgatcccga cgctcgacaa
    13621 cgcgatcttc gccaacacca cccacgccct gcagaagaca ctggacgagg ccggctatac
    13681 gctgctgctc gcctgccacg agttcgaggc gagggcggag gcgcgcatga cgcaggcgct
    13741 gatcgaacgc ggcgtggacg ggctggtact gctcggcgtg agccaccatc cgagcgtgtt
    13801 ccagatgatc gatacgcacc ggatcccgta cgtgctgacc tgggcgctcg acgccagcgg
    13861 gcggcaccct tgcgtcggat tcgacaacag gatggcggcg atccgcatgg ccggtcacct
    13921 gctcgagctg gggcaccgcg agttcgcgat gatctccggc gtgaccgccg gcaacgagcg
    13981 cgcaaccggg cggctgcaag gcgtgcggca ggcgctcgcc gagcgggcga tcgtgctcgc
    14041 cccggaatgc atcgtggaga agccgtacac cttgtccgcc gggcgcgaag gattgcgcga
    14101 ggtgctgcgc gggacgccgc ggcccaccgc ggtgatctgc ggcaacgacg tgctggcgat
    14161 cggcgcgctc gccgaatgcc acgcgcaggg actcgccgtg ccgcgcgaga tttcggtgac
    14221 cggttttgac gatcttgaga tggccgccgt ggtcacgccg gccctcacca ccgtgcattt
    14281 cccgacggcg gaactgggaa gctacgcggc gcagcacctg ctcgctcggc ttgctggcaa
    14341 gccggtcgag atgcgcaccg aattgccggt ggagctggtc gtgcgcgcga gcaccgcccc
    14401 gcctgctata acctaacctc tgccaacaac cgttcgatta ttcgttcggc ttgcgcccgg
    14461 tagccgccgc cgaaaagatt aaggtggttg agcacgtgat agagattgta gagctgcttg
    14521 cgcgtctcgt accccgcgtc gaggggatat tccgcgcgat aggcatcgta gaaaccgcgt
    14581 gggaaaccgc cgaagagttc cgtcatcgca aggtccgctt cccggtcgcc gtagtagacc
    14641 gcgggatcga aaatcaccgg ctcgccgccg gcgtccatcg caacgttgcc tgaccacaag
    14701 tcaccgtgta gaagggaggg atccggatgg tatcccgcga agaacgcggg caatttctcc
    14761 atcaagcgct cgccgttcgc aatcaggcgg ccgccgtggc cctttgatgc cgccagccca
    14821 agctgaaaac gcacccgccg gtcgcgccag aacgcgatcc agtcatcggc cggggtgttg
    14881 gtttgggggg tcgagccgat ggtgttgtcg cggtgccagc cgtaacgccc gcaggtaacg
    14941 cggtgcaggc gcgccagatt ccttcccaag gcacgcatgc tgcggtcgtc ggcaggcctc
    15001 agctcgagat gctccagcac gatccagcta gcggccgggc acgcaccgtg gcaaacgggt
    15061 cgcggcacgc ggaccgtctt cgtgcgaccg atctcttcca gcccggccgc ttcggcggaa
    15121 aacatttccg ccttgtccgg ggcattgagc ttgacgaaaa aggcgcgccc ttgtccgcgt
    15181 acgacgtggc attcgtttat gcatccgccg cctgctccgg cacgcgattc gatagtgaag
    15241 ggcgcgcctg atgcttcgcg aattgagcgt tcgatatcct gccaggggct cacggcgctc
    15301 gaggctggct caagaaaccc gaaaggagcc gcagctgagc gcggtccaaa atcggttgga
    15361 aatcgttacc ctaacgattt ccgggccttg gcgatcgcgg ccttgacgcg caccggcgcc
    15421 gtgccgccga gatgggtgcg gctcttgagc gagccttcga gcgtgagcgc ggcgagcgcg
    15481 tccttgccga tcgccggcga gaagcgctgc aactcggaga tcttgagctc ggacaattcg
    15541 cagccgcgcg actccgcgaa acgcaccgcc tgcgccaccg cttcgtgcgc ctcgcggaac
    15601 gggacgccct ttctcaccag gtattcggcg aggtcggtgg cggtggcatg cccctgccgc
    15661 gcggcggcct ccatggcctc gcggttgacc ttgatggcgg ggagcatctc ggcgaagacg
    15721 gcgaggctca tcgtgagcgt gtccaccgtg tcgaacagcg gctccttgtc ctcctggttg
    15781 tcgcggttgt aggcgagcgg ctgcgccttc atgagcgtca gcagcgccac gaggtgcccg
    15841 ttcacgcgcc cggtcttgcc gcggatcagc tccgccacgt ccgggttctt cttctgcggc
    15901 atgatcgagg agccggtgcg gaagcggtcg gcgagctcga caaacccgaa gcgcgggttc
    15961 atccagagga tgagctcctc gcagaagcgc gacaggtgcg tcatcacgag cgcggcacag
    16021 gcgcagaatt cgatcgcgaa atcgcggtcg gacacggcgt cgatcgagtt ctcgcacacg
    16081 ccgtcgaagc cgagctcctt cgcgaccatc aggcggtcga tcgggaacga ggtgccggcg
    16141 agcgccgccg cgccgagcgg cagccggttg agccgcctgc ggcaatcggc gagccgttgc
    16201 gcatcgcgcg cgagcatctc gaagtacgcc agcaggtggt ggccgaaaga gaccggctgc
    16261 gccacctgca gatgcgtgta gcccggcatc acggtctcgg cgtgctcgtc ggcgaggtcg
    16321 atcagcgcgc gctgcacgga cttgatggtc gcagccgcct ggtcgatcgc ggcgcggagg
    16381 aacaggcgca cgtcggtcgc cacctggtcg ttgcgcgacc gggcggtgtg caaccgcttg
    16441 cccggctcgc cgacgagctc ggtcagacgc cgctcgatgt tgaaatgcac gtcctcgtcg
    16501 gcttccgacc acggaaagcc gccggcgcgg atctcggagc ggatctgcgc aagccccttc
    16561 tcgatcgcgg agagatcgtt tttcgcgata atgcccgttg catgcagcat gcgcgcgtgg
    16621 gcgatcgagg cctcgatgtc gaattcggcg agccgccggt cgaaggagac cgaggcggta
    16681 aagcgctgcg tgagctcggc cgccggctcg ccgaaccgtc ccgaccacgg ctggttcctg
    16741 atcttgtcgt tgtcgggcat gcggcctgtt aaaattcgtg aagcggtttc aaggaccggg
    16801 ctgaagcccg gagggatgcc tgtcagcaat cagccatcag ccatcagctt tcagcgatca
    16861 gccggaggcg cctcaatgaa gctgactgct gagagccgat cgctatacgc cgtcgcatag
    16921 actacatggc gcgaagtata aatcaaaacg cctacaccgg cgccctgccg aacttccgca
    16981 acctcggcat cctgctgcgc atcctgctca tcgtcaacgt gttcgcgttc gcggcggcgg
    17041 tcgtgaaaag cgtggatgcg cgcgcgctgt ggcagcaggt cgtcgaaatc tccgggctgg
    17101 tcgagccgct gctcatcctc tccctgctcg cgcttgccgc gttgaatcac ctattgaggc
    17161 gcatgccgta ctggctcggc gcgatggcgg tgctcgcatt ggagctcgct ctcacgacgg
    17221 cgctctacat cgcaggacgc cccatgctgg gagcggagcc agggccgctg gagcgctact
    17281 ggctgctcgt cgctttcacc accgcggtgc tgctcgtcta tttcgacctg cgcgggcggg
    17341 cgctgtcgcc ggcgctctcg gaagcgcggc tgcaggcgct gcaagcgcga atccgccccc
    17401 atttcctgtt caacggcatc aacgccgtgc tctccctgat ccggcaggat ccccggcgcg
    17461 cggaggcggc gctcgaggac atggccgagc tgtttcgcgt gctgatggcc gacaaccggg
    17521 agctcgctcc gctctcgcgc gaagtcgagc tgtgccgcca gtacctggac ctcgagcagt
    17581 tgcggctcgg cgagcgactg aaggtcgagt ggcgcacgga ccgcatgccg cgcgacgccg
    17641 tcgttccgcc gctggtgctg cagccgctgc tcgagaatgc ggtctatcac ggcatcgagc
    17701 cgcgcgtcga gcccggcgtg gtgagcatcg acatctacgc cgtgggcgac cgggtgcatg
    17761 ccgtcctgcg caacccctac cagcagcagg acgaccacca cgcgggcaac aagatggcgc
    17821 tcgccaacat ccgcgaacgc ctccagctgc atttcgacgc cgaagccacc ctgacggcga
    17881 aggtggctga cgacaccttc caggtgcata tcgtcatgcc ctacatcaaa ggccgtgaat
    17941 cgtgattcgt gagcagtgaa tcgtgagtga ggccaagcca ctgcgggtgg tgatcgtgga
    18001 cgacgaggcg cctgcgcgtt cgcgcatgcg cgatctgctc ggcgaatgcg cggacgaggt
    18061 cccgctcacc attgccggcg aagcggcgag cggacgggcg gcgctcgaat tgctctcgcg
    18121 cgagagcgcc gacgtcgtgc tgctcgacat ccgcatgccg gagatggacg gcatcgaggt
    18181 ggcgcagcac ctgcagcggc tcgagcgggc gccggcggtg gtgttcgcca ccgcctacga
    18241 tgcctacgcg atccgggcgt tcgaggtgca cgcggtggac tacctgttga agccgatccg
    18301 tcttgcgcgc ctgaaggagg cgctcgtgcg ggcggcgcgg ccgcgccccg aggccctgcg
    18361 cgggctggcg cgcgcgccgc gcacccactt gagcgcgcag gagcgcgggc ggatccacct
    18421 catcccggtt gcggacgtga tctacctcaa ggccgagctc aagtacgtgt cggtgcgcac
    18481 cgcggcccgc gagtacctga tcgaggagtc gctcacccgg cttgaggagg aattcggcga
    18541 gcgcttcgcg cgcgtgcacc gcaactgcct ggtcgcgcgc gcggcggtca ggggcttcga
    18601 gcgcgcgggg gaggggggcg aggcgcactg ggaagtgttg ttgaacggcg tggacgagcg
    18661 tatcgccgtc agccgccggc agcagcacat ggtgaaggag ttttcgcgcg gctagtgccg
    18721 ttccaactat attgagctaa tttggtgaaa tggcgtgggc acgaagcaag atgaatggga
    18781 tgcggcaatc gttttgtcga tctaagttgg aacggcacta gccagcatga ccacaacagc
    18841 ctccggaaaa gaagcaccac agcgcattgt gatcgcgtcg cgcgagagcg cgcttgcgat
    18901 gtggcaggcg cgccacgtcc aggcgcggct tgcggcgctc taccccggaa ccggcatcga
    18961 catcgtcggc gtgaccaccg agggcgatcg ccgcctcgcc gcctcgctcg cgaagatcgg
    19021 cggcaagggg ctgttcatca aggaactcga ggagacgctc gcggcggggc gcgcggatat
    19081 cgcggtgcac tcggtgaagg acgtgccgat gacgctgacg tccggtttcg tcctcgccgc
    19141 gatcggcgaa cgcgcggacc cgcgtgatgc cttcgtttcc aatcgccatg ccgatctttc
    19201 cgcgctgccg gcgggagccc gcgtcggtac ttccagcctg cggcgcgagt gccagttacg
    19261 cgcgcgtcat ccgcggttga ggatcgagcc gctgcgcggc aacgtcacta cccggctgag
    19321 gaaactcgac gagggccgct acgaggcagt gatactcgcg gctgccgggt tgaaacgcct
    19381 gggtctcgaa gaccgcatca cgcgcctgct caccccgtcg gagagcctgc cggcgccggg
    19441 acagggagcg ctcggcgtcg aatgcctgga aaaccgcacc ggtttgatcg agctgctcgc
    19501 gccgctcgag cacgctgcca cggcgcgctg cgtcgccgcc gaacgggctt tctcgcgcac
    19561 gctcgcgggc agctgcaacg tgccgctcgg ggcgtacgcg gaagtggaaa acgggaaaat
    19621 ccggttgcgt ggtttcgtcg gcgcccccga cggctcgcgg ctggtgagtg gcgagctggc
    19681 gggagccgcg gatcaacccg aagtgctcgg taccgcgctc gcggagcagc tgaaagcgca
    19741 gggcgccgcc gagatcctcg cgcagctgca aacttgaacc gccaagcaca ccaagtgaac
    19801 caaaattgaa ccgccaaggg cgccaagaat gccaagaaga gcagaaaatg aaaccgcggc
    19861 taaatgcagg tgaacgccga gcgacggaat ttttatgttt ttcccgggcc tcggttgagg
    19921 aggccgactc gagagttccc ggggattgac gataatgaag gaacagtcgc tccgggggaa
    19981 aaacatcgtg gtgacgcgac ccgccaacca ggcgcgggaa ctcgccaggc tgatcgaggc
    20041 cgccggcggc acgcctatcc tgttccctgt gatcgagatc cgcgagctgg aggacccccg
    20101 gccgtttctt gcgctggttg accggctcga cgagttcgac ttcgccatct tcatcagccc
    20161 gaatgccgtc ggccgggcga tgaagctgat tcttcgacgc cgcacgctgc cccgggcgct
    20221 gaaagtgatc gcggtcggcg gcggaaccgt gagagcgctg cgcggttacg gcgtgagcga
    20281 ggtgattgcg ccgcgggacc gctacgacag cgaagcgctg ctcgagatgg ccgagctggt
    20341 ggacgtggac ggcaagcgcg tgatgatctt tcgcggcgaa ggcgggcgcg agctgctggg
    20401 cgacacgctg aaggcgcgcg gcgcgtcggt cgagtatgcg gagtgctacc gccgcggcaa
    20461 gcccgatctc gaccctgcgc cgttgctcgg ggcttgggcg cgcaacgagc tcgatgcagt
    20521 cacggtgacg agcagcgacg gcctgcgcaa cctgttcgaa atggtcggaa cctccggccg
    20581 cgagcgattg gtgatcacgc cgctcttcgc gccgcatccg cgcatcgcgg agaccgcgcg
    20641 cgcgctcggc gtccggaagg tgatcgtgac cggacccggg gacgaaggcc tgctgaccgg
    20701 attgaccgat tacttcaaga agcgtgggtc gtgagtcgta actcgacacg agttacgaga
    20761 tgcgagatcc gagacggcag ataactgctt gttcgcggat tgcgtatcgt gcatcccgca
    20821 tctcgcttcg cggatcacga ttcacgggtt ttcagttgcg gcccgaggct gcccgccccc
    20881 atactatcgc ttgaccgcgc ctttcacgcc ccgcatgaac gattcttccc agcccgaccg
    20941 cccgccggct gccgccggat cgaccgcggg cggcggcgcc gccgagcgcg cgcccgcatt
    21001 gcacaggtct ttgccggttc tgttggtcgc ggcgttggcc ctgggcctcg ccgcatggac
    21061 gtggtacgac agccgcagcg aaatcggcgc gctgcgccag gagcttgcaa agaagctcgc
    21121 cgatgccgac acccagaaca aggaaagccg cctcgtcgcc gagcaggtgc gcgaggcggt
    21181 ggccggcgcg caggtgaagc tcggcgtgct cgagagccgg ctcgccgaat cgcagaacca
    21241 gcagatcgcg ctcgaggcgc tgtaccagga gctctcgcgc aaccgcgacg agtgggcgta
    21301 cgccgaaatc gagcagtcgc tgttcatcgc gggccagcag ttgcagctcg ccggcaacgt
    21361 caaggtggcg ctgatcgcgc tgcagacggc ggatgcgcgg ctgcaacgca tgaaccgccc
    21421 gcagttgacg gcgctgcgca aggcgatcaa ccgcgacatc gagcggctca agtcggcccc
    21481 ccacgtggac acggtggcga tcagcgtgcg gctcgacaac ctcatcgcgc aggtggacaa
    21541 gcttccgctt gcgatggagg tgcggccgca gcccgagcag ccggccgccg cgcaaaaggg
    21601 agccgaggac agcccgtgga cgcggttctg gcgcgacgcc tgggcggaac tcaggcagct
    21661 ggtgcgggtg cagcagatgg accagcccga cgtgcccctg ctcgcgccgg cgcagtccta
    21721 tttcctgcgc gagaacctga agctgcgcct gatcggcgcg cggctcgcgc tgctcgcgcg
    21781 cgacgcggcg agctacaagg cggacctgaa ggccgcgcgc gactggctcg gccgctatta
    21841 cgacacgcgc aacaacagcg tcgcgtacgc gcagggcatg ctgcgcaacc tgcacgatgc
    21901 cgaggtcagc atcgagacgc cggacatttc cgccacgctg gaagcgatgc gcggcgtgcg
    21961 gctctcccgt gaacgcggcg cgcgatgaag acgctcatct gggtcgtcgc gatcatcgtg
    22021 ctggcggtgg cgatcacgct cgctgcgcgc tacaacaccg gctacttgct gctggtgctg
    22081 ccgccgtacc gcgtcgagct ctccctcaac cttctggtcg tgctcgccgt cacgatgttc
    22141 gccgtcggtt acgcgctggt gcgcttcatg gccgccacgc tgcgcctgcc gcggcaggtg
    22201 cgcgaatatc gcctggcgcg ccgcaaggaa aaggcgcagg cgacgcttac cgaggcgctg
    22261 cgcgagtatc ttgcaggccg ctacggccgg gcggaaacgg tcgcagccgc gtcgctcgag
    22321 ctgggcgagc agcccggcct cgccgcgctg ctggcggcgc gcgccgcaca tgagctgcgc
    22381 gcctacgagc gtcgcgacgc ctacctcgac cgggccgcgt cggccgcatc cggcggagag
    22441 gcggcacgca tcgtcacgac cgccgatttg ctgctcgacg agcgccgtta tcaggaagcg
    22501 ctggatctcc tcaagctgct gccgcgccgg cacaccgcgg cgctccgtct cgaattgaag
    22561 gcgcagcagc aggcgcgcaa ctgggagcag gtgttcgcgc tggccgagga attgcgcagg
    22621 cgcaacgtgt tcgacgcgac ccaggcgggc gaggtgcgcc gctacgcgct ggcggagaac
    22681 ctcaaacgca aggcgctcga ttcgcgcgcc ctggaggaag cctgggagaa ggtgccgtca
    22741 tccgaccgca aggaccggcg cgttgcggct gccgcggcgc aatgcttcat cgcgctgggc
    22801 ggctgcgaca aggcgcatcg catcatcgag gaagcgctgg aagcgggctg ggacagcgat
    22861 cttgtcggtc tctacgccga atgtgagggc ggcgacaccg tgaggcgcat cgagcgcgcc
    22921 gagacctggc tgaaaatcca gccgggcgat gccgcgctgc tgttgacgtt gggtcggctg
    22981 tgcgcggatc aggggctgtg gggcaaggcg cagagctatc tcgaggcgag cctggcgatc
    23041 gagccaaccc acagcgccca tctcgcggca gcccggctgc aggagcagct gggcaacgcg
    23101 gaagcggcgc ggaggcatta ccgcgagagc ctcgacctcg cgctcgcgca gttgaagacc
    23161 gtcaccggcg gacgccgccg aagccagtta taaccgctca ccgcggagga cgcggaggac
    23221 gcagaggacg gagaatgagt cgaatcacga ctcacgccgt tccggcctag cggtcgaaat
    23281 cgatgtcccg gtacgactcg ggatagccga tcggctccac atgggtcagc accaccgcgt
    23341 cgggcagttc cttgcggata tcctcctcga cctgatgcgc caggtcgtgt ccctgcgcga
    23401 ccgtccagtc ggccggcacc agcacgtgca gggtcacgaa cgagcgcgca ccggcctgac
    23461 gcgtgcgcag cgcgtggaag tccacgcccc gggcgcggta gcgatcgagc acgcgatcga
    23521 gaatcctgag ctgctccggc ggcagcgcac ggtcgagcag gcccttgatc gagcgctgca
    23581 tcaattcgta gcctgaccat acgatgttgg cggccaccgc tagcgcgatc agaggatcga
    23641 gccgctccca gccggtgacc cacacggcgg ccacgcccac gatcacgccc gcggaagtcc
    23701 agacgtcggt catcaagtgc cgggcgtcgg cttcgagcgc aatggaccgg tagtcgcggc
    23761 cgccctcgaa caggcggcgc gccaccgcat aattgacgag cgcggcggcc acggaaaccg
    23821 ccagtccggg cccgacgtgc tcgagcgggc gtggagcgat gaggcggtcg actgcggcaa
    23881 cagagatggc gatcgccgcg atcaggatca gcgtaccttc gaggccgctc gcaaagtact
    23941 cggccttggt atagccgaag gcatggtcct cgtcgggtgg acgcgccgcg ataccgagca
    24001 tcgccagcac gaccgtggcg ccggcgagat tgatcgtccc ttccagcgca tcggagagca
    24061 ggccgaccga gccggtgacc caccacgcca gcgtcttgag gccgatggtg gcgactgccg
    24121 cggcgatgga caacaaagcg aattttcggg cgccaggatg cgacatgagg cgacagggat
    24181 gagggcggag ggatgaagga tgaggaaaaa accttctcgg gtcgactctg gcttttctct
    24241 atccctgcgt cctccgcggg aggagttgct gctttacccg gcggcaggtt ttcagtttcc
    24301 gaacgccgca ggcgtgaagg aatcggagaa aaactcctct tccggcagcc cgcaccggga
    24361 agtgaaatcc ttgtgcgcgg cttccaccat taccggcgcg ccgcaggcgt agacctgata
    24421 gccggacagg tcgggaaagt cctccgtcac tgcgcggtgc acgaatcccg tgcgcccggt
    24481 ccagttgtcc gtcggcaggg cttccgagag caccggcacg tagcgcagtc ttccctcgcg
    24541 ctgccaacgc tcgggaagtc cgtgcaggta gagatcggcg cgcacccggc agccccagta
    24601 gagcgtcatc gggcgcttca cgcccgtgtg cagcgcgtgc tcgatgatcg ccttgatcgg
    24661 cgcgaaaccg gtgccgcttg cgaccaggac gatcggcttc gcgctctcct cgcgcaggaa
    24721 gaaggtgccg aacggccctt cgaggcgcag gatgtcccgc tccttcatgg ttccgaaggc
    24781 atgccggctg aacggcccgc cgtaattcct gagatgcaac tgcaggaagc cttcatcgtg
    24841 cggcgggttc gccatcgaga agctgcggcg cgtgccgtcc cgcagcagga gatccacgta
    24901 ctggccggcg aggaaccgca gtttctcgtt cgccggcagc ttcaactcga gcagcatgac
    24961 gtcgggtgcc gcgcgctcca tcttcatgac gcgggtgggc agcatcttga tctggatgcc
    25021 cttcaccgcc gcgccgatct cgcggcactg gatcaggaga tcagagtgcg gcttggcctg
    25081 gcagaacagc gccatgccag cgcgcttctc cgcctcggac atggcctgct cctggtaggt
    25141 gccgtaatcc acgctgccct ccagcagctt gcccttgcag gtgccgcagg agccgtgccg
    25201 gcagccgtac gcgatgacga taccctcgcg cagcgcggcg gcgagcacgg tttcgccgtc
    25261 gctgacggaa aacgcgtgcc cgctcgattg aatcgtgacc ttgtggggca tgggctcgtc
    25321 cggcgcgccg cccggggcgc atgctcgatt aagctagaat tcccgtatga ggcatttact
    25381 cagcgccgcg acaaaacggg gcagcagtct accacggtac cccgcccgca tcagtacgag
    25441 cggcgcctgc ggcgatcccg tcacggaggg cgctcatgga tgaagtggtg gtcttgctca
    25501 ccaatcttcc cgaccgggac acggcgctga agctcgcgcg ggagctcgtg gcgaagaaac
    25561 tcgcggcctg cgtgagcgtg ctcgcggaat gcacctcggt ctaccgctgg aagggggagc
    25621 tcgaaaacgc cgtcgaggtg ccggttctga tcaagacgcg cgcggcccgc tacgacgagg
    25681 tcgaggcggc gatacgcgcg cttcatccct atgaacttcc agagattctc gcggtcccag
    25741 cggtgcgcgg cctggacgag tacctgcaat gggtggcgga cgaaaccgcg attcgtgagt
    25801 cgtgaatcga gcagggtaaa gtgatgacgc tgggttttta ccaatcacca atcaccaatc
    25861 acgaatcacg cctctggcgc ctgccatgcg cctcgtactg atcttcctgc tcacattttc
    25921 gtgcctcgcg cgcggcgcgg agcccgagct gcttgagccc gagaaggcgt tccgcttctc
    25981 ggcccggctc aaagacgagc gctcgatcga ggtgcgctac cagatcgcgc ccggctatta
    26041 cctctaccgc gacaaattcc gcttcgcgct cgccccggaa gacgcgaagc tgggcgcggt
    26101 ccagttgccg gccggcaaga agcatcgcga cgatttcttc ggcgaagtcg agacctatcg
    26161 cggagatctc cgcatcacgc tgccgttcga ggccgaccgc gacggcgtgc cggcggtcac
    26221 gctcaccgcg atctcgcagg gctgtgccga tatcggtgtg tgttacgtgc cgcaggagca
    26281 gaaggccgtg ctgcggccgg ccgccctcgg cgccgcgggc acgcgcagcg atgcgtcctc
    26341 cacgccgttc ggcgccgcgc cggccgcagg cgaggacgcg cgcctggccg cgctgtttga
    26401 cggcgggttc tggctggcgg tggcgagctt tttcggtttc ggtctgctgc tctcgttcac
    26461 gccctgcgtg ctgccgatgg tgccgatcct ctcgggcgtc atcgtcggcg ccgggcactc
    26521 catcacccgc gtcaggggtt tcctgctcgc cgccatttat gtattcggca tggcgatcac
    26581 ctacgcggtt gcaggggtcg cggcgggact gtccggggag atgctctcgg cgacgctgca
    26641 gaacccgtgg gtgctggggg cattcgccgc cgtattcgtt gcactggccc tggccatgtt
    26701 cggcttctac gagctgcaac tgcccgtggc cctgcaaagc cggctggccg acgcgagcaa
    26761 ccggttccat ggcgggcact ttgcgggcgt gttctcgatg ggcgtgctgt cggcgctgat
    26821 cgtcggcccg tgcgtggcgg cgccgctcgc gggcgcgctg ctctacatca gccagagccg
    26881 ggacgtcgtg ctggggggcg ccgcgctctt tgcgatggcg ctcggaatgg gcgtgccgct
    26941 cctcgtggtc ggcgcctcgg ccggggcgct gctgccgaag gcggggccgt ggatggaaac
    27001 gatgaaacgc ttcttcggca cgctgctgct cggcgtggcg atctatcttg tctcgccgtt
    27061 cgttccgctc ccggtgcaga tgttcctgtg ggcggcgctg ttgatcgtga ccgggatcta
    27121 cctgcgcgcc atcgaccccc ttccggtcaa cgcccacggc ttccaaaagt tcagcaaggg
    27181 cgtcggcgtg atcgcgctca tcgcgggagt cgcgtacctg atcggggcgt tttcgggcgg
    27241 gcgcgacctc ctccagccgc tgtccggcct gcggctcacg gcctctgaaa gcgggccgca
    27301 cgagacgccg ttccagcggg tcagcaacgc agccgagctt gatgcccgcg tcgccgccgc
    27361 agccggccgg ccggtgatgc tggacttcta tgccgactgg tgcgtctcgt gcaaggagat
    27421 ggaacgctac accttcagcg acgagcgcgt gcgggcccgg ttccgcgaga tggtgctgct
    27481 gcaggccgat gtcaccgcca acaatgccga gcacgccgcg ctgctcaagc gcttccggct
    27541 gttcggcccg cccggcatcg tctttttcga ccgtgaaggc cgcgagatcc ggggcctgcg
    27601 cgtgatcggc ttccagcccg ccgaccggtt cgcggcggtg ctcgaccagg ccctgaattt
    27661 ccggtgagga aagcgcgcgc cctcgcgctg tttgccgcgg tggcggccgc agccgccgtg
    27721 gtcggcggcg ccgcctacca cctatggctg cgtggcggcg ccccggcccc tcaggcaatc
    27781 gtcgagcggg tgtatgcggc gcggctcgcc gacctgaagg gcggcgccca gaccctggcg
    27841 caatggcggg ggcaggtgat ggtggtgaat ttctgggcca cgtggtgtgc cccatgccgg
    27901 gaggagattc ccgggttcgt gcggatgcag gagcgctatg gctcccgcgg cctgctgttc
    27961 gtcggcatcg ccttagacca gccggataag gtggccgagt tcgcccgcga gttccgcatc
    28021 aactaccccc tgctgatcgg cggattggat accttggagt tgctgcgcca ggcgggaaat
    28081 cgcgccgggg tcctgcccta taccctggtg atcgaccgca ggggcaattt ggtgagccgc
    28141 gagcctggcg gcctcaagga agcccggctc gagggtatta tcctgcctct cctttgacct
    28201 ctcttcgcaa gcttacattg cacaacttcg gctaatttcg cccaagtatt gcgtttaact
    28261 gtcgctactg tcctttcttg aaaaggctca cccacccatt ttttgtgcat cagacctccc
    28321 gttccaggtg atgggggaac ggcgctgatt tgacggtgat ttttcaatta attacccatt
    28381 aacttcgccc attttatgga caatttcgtt gattttgggc aaactttcag ccatcgtcac
    28441 cttctgacac ggatccgccc cgattttgat agggagggcc gtgaaatcct cgcaaatcgc
    28501 catggccggc atcaaacaca tcctggtcat caacggaccc aatctcaatc tcctggggtc
    28561 gcgcgaaacc gagcactacg gaagggacac actgaaggcg atcaatgccc ggctcgccca
    28621 gcgcgcccga aaggcgaagg tgcggcttgc cacttatcag agtaattccg aggcggcgct
    28681 ggtggcgcgg gtccagcagg ctcgcaggga gaaggtggac ttcatcatca tcaaccctgc
    28741 ggcctacacc cacaccagcg tggcactgcg cgacgccctg gcggcggtcg gcatcccttt
    28801 cgtcgaggtg catttgtcga acgtctttgc ccgggaatcc ttccgccgcg aatcgtattt
    28861 cagcgacatc gcggttggca tcctcagcgg gctaggcgcg aagggctacg aactggcgct
    28921 ggagtttgcc ctgcagtatc agaaaaaggg gtgaccatgg acctccgaaa gctcaagacg
    28981 ctgatcgatc tcgtgcagca gtcgggcatt gcggagctcg agatcacgga gggcgaggag
    29041 aaagtgcgca tctgccgcgg cgtcgtcggg ggccagccgg cggcgcccgc gacgcaggtg
    29101 gtgatgctgc cgcaggcggc ggggatggcg cccgccgtgg ccggcggcgc cccggcggca
    29161 ccagccgaga gcgaggccgc ggctgcgccc gaggggcatg tcatccgttc cccgatggtc
    29221 ggcactttct accgctcctc cgcccccggg gcgaagccct tcgtcgaggt cgggcaggcg
    29281 gtcaaggcgg gcgatacgtt gtgcatcatc gaggccatga a
//
LOCUS       DBNGWZ010000121         5622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2782752, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000121 DBNGWZ010000000
VERSION     DBNGWZ010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2782752"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2979
                     /gene="rpoB"
                     /locus_tag="AC16CX_03650"
                     /pseudo
     CDS             1..2979
                     /gene="rpoB"
                     /locus_tag="AC16CX_03650"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            3321..3749
                     /gene="rpsL"
                     /locus_tag="AC16CX_03655"
     CDS             3321..3749
                     /gene="rpsL"
                     /locus_tag="AC16CX_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR3011640.1"
                     /translation="MPTINQLVRKGRQSKKTKSKAPALQYTLNTNKQRRTVQPKGAPQ
                     KRGVCTVVRTQTPKKPNSALRKVARVRLTNGLEVTAYIPGEGHALQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTTGVEARRQGRSKYGGKRPKVAKT"
     gene            3828..4298
                     /gene="rpsG"
                     /locus_tag="AC16CX_03660"
     CDS             3828..4298
                     /gene="rpsG"
                     /locus_tag="AC16CX_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3011641.1"
                     /translation="MPRRNRPAARALPPDARYHSLVVQEFINRMMRRGKKSVATGVMY
                     RSLDLVSERAKRDAMEVFDQALKNVAPMIEVKPRRVGGATYQVPVEVPPDRRMTLAMR
                     LILAAARTRGGKSMAEKLAGELLDAANNTGSAIKKREETHKMAEANRAFAHYRW"
     gene            4392..>5622
                     /locus_tag="AC16CX_03665"
     CDS             4392..>5622
                     /locus_tag="AC16CX_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3011642.1"
                     /translation="MPGKYPLEKVRNIGIIAHIDAGKTTTTERVLFYTGKTHRIGSVD
                     DGTTVTDWMEQERERGITIVSAAVTAEWRDHLFNLIDTPGHIDFTAEVQRCLRVLDGG
                     VVVFDGVQGVEPQSETVWRQADRYGVPRLCFINKMDRIGADFNYSVQTIKERLGANPI
                     PMQIPVGRESSFRGVVDLLENAAVVWADDLGTKMERVEIPDELKAPAAEARAQMVEAI
                     SETDDQLTVKYLEGQEITVPELRAALRRAVIAGKCTPVFCGSALKNKGVQPMLDAVVD
                     YLPSPLDIPPVKGTNPNSEETVERRAEVKEPLAALVFKIVSDPYVGRLAYFRVYSGKI
                     KTGTMVLNSTKGKRERIGRLIRMYADRREDIEEVEAGDIAAVLALKDTFTGETLTDSA
                     NPVVLEAITFPDPVIDVA"
BASE COUNT         1149 a         1687 c         1885 g          901 t
ORIGIN      
        1 gacccgccga ccctcgacaa cgcccgcgaa tttctcgagc agcagttgtt cgatcagcgc
       61 cggtacgacc tcgagcgggt cgggcggtac aagctcgacc agaagctcaa tctgcaggac
      121 gtggtcccgc tcacgcaccg gacggtgacg aagcacgacg tcattcagtt gatctcgcgc
      181 atgatccgga tcaacaacgg cgtggaagag aaagacgaca ttgaccatct cggcaaccgc
      241 cgggtgaaga cggtgggcga gctcatccag aacaagatgc gcatcggcct gcgccggacg
      301 gagcgcgtgg tgcgtgagcg catgtccatt cgcgagcagg acaacgtcac gccgctcacg
      361 ctcatcaaca tccgcccggt cgtcgccgcc attcgcgaat tcttcggctc cagccagctc
      421 tcgcagttca tggatcagac caacccgctg gccgagctga cgcacaagcg caccctctcg
      481 gcgctcggcc ccggcggcct tcgccgcgag cgcgcgggct tcgacgtgcg cgacgtccac
      541 cattcgcact atggccggat ctgccccatc gagacgccgg aaggcccgaa catcggcctg
      601 atcggccgcc tcggttcgta cgccaaagtc aacccctttg ggttcatcga gacgccgtat
      661 cgcaaagtcg tgaaggcgct caaaccggat gataagcgcc tgctgggccg caaactgcgc
      721 gaagaggtcg ccgacgcgga gtcgggcgag gcgctgggcg cgtcggggga cgaagtcacc
      781 cgagaactgg cccggcggtt cgcaaaggcg gacctggagg ttccgatcat cccgttcgtc
      841 tcggatgaaa tcgtctacct gtcggcggac gccgaagacc gctacaccat cgcgcaggcg
      901 acgacgccgc tgaacgaaca gatggaattc ctgcgcgccc gcccttcctg ccgccatgcc
      961 tcccggttcc tgtttgcgac cccggatcag attgactaca tggatgtcgc gccccggcag
     1021 atcgtaggca tcagcgcggc cctcatcccg ttcctcgagc acgacgacgc caaccgggcg
     1081 ttgatgggct cgaacatgca gcgtcaggcg gtgccgctgc tgcgccccga agtgccgatt
     1141 gtgtccacgg gcatggaggc ccaggcggcg ctggactcgg gacaggtgat tacggcggaa
     1201 gaagacggcg aggtcatctc ggtcacgggc aaagagatcg tggcgcagag cggcggcaag
     1261 aagcgcgtct atcgcctgcg caagtaccac cgctcgaatc agagcacctg catcgaccag
     1321 cgtccggcgg tgagcaaggg ccagcgcatc aagaaaggcg acgtcatcgc cgactcgtcc
     1381 tcgaccgatc agggcgaact ggcgctgggc cacaacgtgc tggcggcgtt catctcctgg
     1441 gaaggcggca actacgagga tgcgatcctg atctccgagc gcctggtgcg cgaggacaag
     1501 ttcacctcgg tacacatcga gaagcacgaa gtcgaagcgc gtgacacgcg gctcgggtcg
     1561 gaagagatca cctatgacat cccgaacgtg ggcgaggagg cgctgaaaga tctcgacgag
     1621 cacggcatca tccgcgtggg cgcggaggtc gggccgaacg atatcctggt gggcaagatc
     1681 acccccaagg gcgagaaaga gctcacgccg gaggagaaac tgctccgggc catcttcggc
     1741 gagaaggcgc gcgaagtgaa ggactcgtcg ctgcggttgc cgcacggcga gaagggcaag
     1801 gtcgtggatg tcaagatctt cacccgcgac gagcatcgtg acctgccggc gggggtcgag
     1861 aagatggtgc gcgtctcggt cgcacagaag cgcaagctga cggagggcga caagatggcg
     1921 ggccgccacg gcaacaaggg cgtgatctcg aaggtcgtgc ccatcgccga catgccgttc
     1981 ctcgaagatg gcacgccgat tgacatcatc ctcaacccgc taggcgtgcc gggccgcatg
     2041 aacatcggcc agattctgga gacgcacctg ggctgggccg ctgaccggtt gggcttccgc
     2101 gccgtcaccc ccgtattcga tggcgccgac gagggcgaga tcgaggcgga actcgcgcgc
     2161 tcgtggctga ttgaccgggc gtggcgggac atcaccgacc aggcgtgggc gtggctgcag
     2221 gagcaggaat acgacccgga gagcatcgag aacgacggcg aggcgcgccg gctgtatctg
     2281 gcgcaatggc tgcgcgggaa aaaatacaac caggatttgc tggcgacgga cgagccgtat
     2341 gcccgccgcg ccgcgctgcg tgaatggctg cgcgagaagg aatacaaccc ggacgaggtg
     2401 gtcgtgttcg aagacagccc gctgacaggg gctgagcgca aggtagcgga ccagctggcg
     2461 gtagatgtgt gtctgcggct gtggatggag gatcacggcc acaaggggaa ccgctggagc
     2521 ggggaggatc tgcgccggcg agctgaggcc gtttcgctgg aggtcaataa accgctgccg
     2581 atcctgggca agcaggtgct gtatgacggg aagaccggcc tgcggtttga ccagccggtg
     2641 acgatcgggt tgatccacat gctgaagctg gcccacctgg tggaagacaa agttcacgcg
     2701 cgctcgaccg gcccgtactc gctggtcacc cagcagccgc tcggcggcaa ggcgcagttc
     2761 ggcggccagc gcttcggcga gatggaagtg tgggcgctcg aagcctacgg cgcagcgcat
     2821 acgttgcaag agatgctcac cgtgaagtcg gacgacgtgc aagggcgcgt gaagacgtat
     2881 gagtccatca tcaagggcga gccgattgaa gagccgggca tccccgcctc cttccgggtg
     2941 ctggtgaaag agctgcagag cctcggcctg gtcgtggaag ccgtggtgga gggcggcgag
     3001 gtcatccgct tcggcaagga ggaagagaaa gggcggacgc ccaggcccgg agtcggtctg
     3061 ctgaatatgc tggacgattg aggtctgagc gcgctggcgt ccaggaaaaa tttcacaaca
     3121 ccttgacagg ctctcggccc gtatggtaaa ctgacgatcc ttgtgagcaa tttcagtccg
     3181 tgagggccac ccagtcaagg tcttcacgag ttgaatatag aaataggtga ggcgaggcca
     3241 tcgtcgtact ttgcgatggc ctcccttact gccaaaagcc cgaaatgtaa gtttcgagtt
     3301 aggaagtccg gagacaaagc gtgcccacca tcaaccagtt ggtccgcaaa ggacgtcaga
     3361 gcaagaagac caagagcaag gccccggcgc tgcagtacac gttgaatacc aacaagcagc
     3421 gccgcaccgt gcagcccaag ggcgcgcctc agaagcgcgg ggtttgcacc gtggtccgca
     3481 cccagacgcc gaagaagccg aactcggccc tgcgcaaggt cgcgcgcgtg cgcctgacca
     3541 acgggttgga agtgacggcg tacatcccgg gcgagggcca cgcgctccag gagcactcgg
     3601 tggtgctggt gcgcggaggc cgcgtcaagg accttccggg ggtgcgctat cacattgtgc
     3661 gcggcaccct cgacaccacc ggcgtcgaag cccgccgcca ggggcgctcc aagtacggcg
     3721 gcaaacgacc caaggtagcc aagacctaat tcggtaacgg gtcaatgagc aggaggaaat
     3781 cccctcgttg gcccgttact ctggtttatt gattacggag ttttcgcatg ccacgtcgca
     3841 atcgccctgc cgcacgcgct ctcccgcccg atgcccgcta ccacagcctc gtcgtccagg
     3901 aattcatcaa ccggatgatg cgccgtggga agaaaagcgt ggcgaccggc gtgatgtacc
     3961 ggtcgctgga tctggtgtcc gagcgcgcga agcgagatgc catggaagtc ttcgatcaag
     4021 cgctgaagaa cgtcgcgccg atgatcgagg tcaagccgcg ccgcgtcggc ggagccacgt
     4081 atcaagttcc cgtggaagtg ccgcccgacc ggcggatgac gctggccatg cgtttgattc
     4141 tcgccgccgc ccgcacgcgt gggggaaaat ccatggccga aaaactggcg ggcgagttac
     4201 tggacgcggc taacaatacc gggtcggcca tcaagaagcg cgaagagacg cacaagatgg
     4261 ccgaggccaa ccgcgctttc gcacattatc gttggtaacg taaagtgggt tgttaggtac
     4321 gcctggggcg ggttgctcag cgacctacgc ctgcaaccgc gctgagtttc gttcactgcc
     4381 tgacacctgc tatgcctggg aagtaccctc tcgagaaagt acggaacatc ggcatcatcg
     4441 cgcacattga tgccgggaag acgactacca ccgaacgcgt cctcttctat accgggaaga
     4501 cgcaccggat cggctcggtc gacgatggca cgacggtcac cgactggatg gagcaggagc
     4561 gcgagcgggg catcaccatc gtctcggcgg cggtgacggc ggagtggcgc gatcacctct
     4621 tcaacctgat tgacaccccg ggtcatattg actttaccgc cgaggtgcag cgctgtctgc
     4681 gcgtgctcga cggcggggta gtcgtgttcg acggggtgca gggtgtggaa ccccagagcg
     4741 agaccgtctg gcggcaggct gaccgttacg gcgtgcctcg cctctgtttc attaacaaaa
     4801 tggatcgtat cggcgcggac ttcaattatt cggtgcagac gatcaaagag cggctgggag
     4861 ccaaccccat ccctatgcag atcccggttg gccgcgagtc gtcattccga ggggtggtgg
     4921 atttgctgga aaacgccgcc gtcgtctggg cagacgacct cggcaccaag atggagcggg
     4981 tcgaaatccc ggatgagttg aaggccccgg ccgccgaagc gcgcgcgcag atggtcgaag
     5041 cgatttccga gactgacgac caactgacgg tcaaatacct cgaaggacaa gaaatcaccg
     5101 tgcccgagct gagggcggcg ctgcgccggg cggtgatcgc cggcaagtgc acgccggtct
     5161 tctgcggctc ggcgcttaag aacaagggtg tccagccgat gctggacgcg gtggtggatt
     5221 acctcccctc gccgctggat atcccgccgg tcaagggcac caacccaaac agcgaggaga
     5281 ccgtggagcg ccgcgcggag gtgaaagagc cgttggctgc gctggtgttc aagatcgtct
     5341 ccgacccgta tgtgggccgc ctggcttact ttcgggttta ttcaggaaag atcaagacgg
     5401 gcacgatggt gttgaattcg accaagggca agcgcgagcg cattggccgg ttgatccgga
     5461 tgtacgccga ccgccgggaa gatatcgaag aggtggaagc cggggacatc gcggcggtgc
     5521 tggccttgaa ggacaccttc acgggcgaga cgctgaccga ctcggcgaac ccggtcgtgc
     5581 tcgaggcgat cacgtttccg gacccggtga ttgacgtggc gg
//
LOCUS       DBNGWZ010000122        16017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1649763, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000122 DBNGWZ010000000
VERSION     DBNGWZ010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16017
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1649763"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16CX_03670"
     CDS             complement(<1..271)
                     /locus_tag="AC16CX_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011643.1"
                     /translation="MTHRNEDDGRAHAAATRGTRLLSSIACAALAALGLSHGTSPASE
                     PAASYPAKPVRLIVAQGTGSSVDNMGRVLAMRLTEEWGKQVIVDNR"
     gene            396..1382
                     /locus_tag="AC16CX_03675"
     CDS             396..1382
                     /locus_tag="AC16CX_03675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011644.1"
                     /translation="MSTERLAVSLALALLTATPAPVHAQRESGSQAYPTRPIRLIIPY
                     SPGGASDNIARLVMPRLGEALGQTIVIDNRPGGAGSIGRDLAAKAAPDGYTLLSTDAP
                     HAINVHLRKVPYDSLKDFTTITTTATSPMIMIVYPGFPAKTVKELVAIARAQPGKLHF
                     GSGGTGAITHMTGELFKLAANVDIVHVPYKSIAPAVTDLIGGQIPIAFPGTATVGGHI
                     RAGRVRLLAVAAKQRSPAFPEAPTFEEAGVPGVVAANWYGIMGPAKLPRPIVNRLNAE
                     LHKVVRLREIQERFAASGIETLLNTPQEFQAMLESETARWGEVVKAAGVKPE"
     gene            1401..1745
                     /locus_tag="AC16CX_03680"
     CDS             1401..1745
                     /locus_tag="AC16CX_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011645.1"
                     /translation="MYESRADAPLSRPRFLRRLSWHVVAAVGLLLGSLLLGMGGYMYF
                     ENLAWRDAFLNASMLLGGMGPVDAPKTNGGKLFAGMYALYSGLVFLVAVGVALAPIVH
                     RILHKFHWKQNA"
     gene            2079..2711
                     /locus_tag="AC16CX_03685"
     CDS             2079..2711
                     /locus_tag="AC16CX_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011646.1"
                     /translation="MKKTMLCLCVWGWAAATAVAAERWSLEAYLGDAYNFRSTLKIKQ
                     DGGFSRSLTADYDTNGFDTPQYYTLRGSRWEQDRAWELSLIHHKLFLNNPPAGVAALS
                     VSHGFNILTVNRAFRSGNWVYRFGAGPVITHAEGIINGISYDGPYKLSGAALLAGGGR
                     RFYAGKSTFLSLEAMATAAYASPEMSGPLNAEIKVTNVAIHGLAGIGFEF"
     gene            2890..3480
                     /locus_tag="AC16CX_03690"
     CDS             2890..3480
                     /locus_tag="AC16CX_03690"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3011647.1"
                     /translation="MKAALIVFDRMTYLDFVGFYDPVTRLKSMNIIPDFEWRICSNKK
                     RVVDDRGATGMTMIPDSVMEPLNGYDLLFVPGGLGTRELQSDREFIEWLKTAAPARPK
                     ISVCTGALLLGAAGFLRGKRATTHPSALKELEPYCDAVLTDRIVDEGDVITGGGVSSS
                     IDLGLHVVHQLAGPEARARVAKQMCYPYQWKDQVVR"
     gene            3507..4043
                     /locus_tag="AC16CX_03695"
     CDS             3507..4043
                     /locus_tag="AC16CX_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011648.1"
                     /translation="MPHEHELEAAERAAIIAEWERLRAEPRPPAPPPYGCATFLVAAA
                     LLLIVPQLLKLAGWELPRPLELVALVVLALAMAGGLFVGIFVGSGIFGRASNRANAAI
                     DWLEANPGSSDPEERRRQAVTLLFNAVVSDGPTTSGTFDPDRARERLGANLPYVLAVE
                     RALVEERGLWVVFGEKSK"
     gene            4211..5197
                     /locus_tag="AC16CX_03700"
     CDS             4211..5197
                     /locus_tag="AC16CX_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011649.1"
                     /translation="MIRPALCHATAAILALSLLAASAAGAQKTDWPTKPVRFVVPFPP
                     GGSNDQLARLLGARLAETHGQPFVVDNRQGASGAVGTAVVAKAPADGYTYLFAFDTHA
                     INPALYRNLAYDTFRDFTPVMLIGTAPQCIAANSTSRFKSMAEVIRAAKARPDSVSYG
                     STGNGTLGHLMMMLLQKEGGFKAVHVPYKGFAPMAQDVMGGQLDFGLGTVAVLSPFVA
                     SGRLRGLAVSSETRSLAMPDVPTLSEQGFPGFSGLSWWGIFAPANTPKPVVAKFHAEL
                     ARIINAPELRKRLSEQLGMTVVTSSPEALQKFVVSEASRWGKVIKDNQIRPD"
     gene            5325..6326
                     /locus_tag="AC16CX_03705"
     CDS             5325..6326
                     /locus_tag="AC16CX_03705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3011650.1"
                     /translation="MIRAAIVGLGWWGKTIVESVQGKSDQITFVAANTRTRAKAEEFC
                     RDKKIDLRDSLDDILKDPKIEAVVYTTPHSQHEEHVRRAAQAGKHVCMEKPFTLTAAS
                     ARAAIEAVKKAGVVLAVDFQRRFHPSVAEIRKRVRDGSLGPISFCVGEVSTPSGLALP
                     KESWRVNPEETPAGAMTGLGVHLVDAFIDLCGEVEEVYCINARRAAPLVDDTTVFTMK
                     HKNGVISTNYCTLASAASYCIAVFGKNGIAETARPSLDTFRFMPVAAQGGPHATAQPE
                     VIENKGVNLAKAVMEAFAAAVQGKAPYPVTHDQIVHGVAVFEAIVKSAKTGRPVKVR"
     gene            6405..7379
                     /locus_tag="AC16CX_03710"
     CDS             6405..7379
                     /locus_tag="AC16CX_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011651.1"
                     /translation="MPLPVKFAALVFAAHIAAAYAVSHAQAWPNKPIRMLVGFPPGGP
                     TDVVARIVSEKVGGQINQRIVIDNRPGAAGNIAVEILAKANPDGHTLLYSSNAIALSP
                     GLYSKLGYDPLKDLAPVTEIGAGCLIFVVHPSLPVQSVQAFIDYAKARPGQLNYASSG
                     SGTSTHLAAVLFSQRTGIQTQHVPYKGTAPSLVDMIAGRTQFLMGAVLTAVPHIKEGR
                     LRALAVTGSKRIKSLPEVRTLEESGLPGFVVTTWQGVFAPAGIPAAVLAKVNAEFVKA
                     VHSPELRPRIEQQDMDPTGLAVKAFTAAYYAELKRWTKVARDAGLKAD"
     gene            7382..8104
                     /locus_tag="AC16CX_03715"
     CDS             7382..8104
                     /locus_tag="AC16CX_03715"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3011652.1"
                     /translation="MSDPFEDHCWKDVIPADVLEIYSYYRREVSVGPNPALLAVDLYE
                     LVYQGGPRPVAEVARTYPSSCGEYAWAAIEPTRRLFAAARAACIPVAYSTNETRPDSR
                     ATPVWSTKRQRVPIGPELYAIRPEFQPQPGDIVIHKMRASVFFGTPLIAHLTQLGVQT
                     VIICGESTSGCVRATAVDAYSHGFHVVLVEECCFDRSLLSHKVNLFDMHHKYADVIGV
                     DAVTAHLKTIKNLRGRAAKQNG"
     gene            8133..9116
                     /locus_tag="AC16CX_03720"
     CDS             8133..9116
                     /locus_tag="AC16CX_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011653.1"
                     /translation="MNKRLRISVTVGLAAALIAAAPHAAAQSAAAYPTKPVRLIVASG
                     AGGGLDFVARQVATPLSEAVGQTVVVDNRAGASGSIAAELASQAAPDGYTLMFLSASL
                     VVYGIVNKTRYDLYRDFAPVSQVAAAPYLLTVYPGLPVKTVKELVAYAKAAPSKLNYS
                     STGNASLAHLAAELFRVSTGTELVHIPYKGVGAALPDMLSGRVHMSFLSMGSVFSQVR
                     TQKLKAIAIASAARAKMAPEIPTMIESGVPGFVVTQWHGMLAPRGTARPVIERLHREL
                     VKAVQRPEVVSRLALDGTEGIASSSEEFGKHLRSEREQWAKVVKLANIRGD"
     gene            9172..9759
                     /locus_tag="AC16CX_03725"
     CDS             9172..9759
                     /locus_tag="AC16CX_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011654.1"
                     /translation="MRSGLFIAAVLLAISPGAGIAQKLPADCSTAAAPKQPLEISVRG
                     TKFTPKSVKLRSAGGVTYGDEQFDTYRLSMRSEDELSPPLESEVTILVRKGQRIDGRV
                     FRKLPTKETGKQPVPTKGLPEVQGWSFKNRPARTDSSHVEHVGSLRLEFGQRQGASIN
                     GNIYLCVAKGQTTIFDSTPTKEDSYAVGTFQARIE"
     gene            10189..10881
                     /locus_tag="AC16CX_03730"
     CDS             10189..10881
                     /locus_tag="AC16CX_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011655.1"
                     /translation="MPAARFFGLFFLVLTAGCAGIGCAPPREPPQTGATQGGAATIEP
                     PQAGAPQKAATAVKPLPSTAVTQGEAVAVKPEARPATPSTTGPTGESVAKNDAPGAKT
                     PAKVPATAAPKEPLPKKESAAPAPAKPAAPPSLDLTSLEKRLRETDAIGVMTKLTLKN
                     QVDDLLNRFRAYYEGRIKTTLAQLRQPYDTLILKVISLLQDKDPSLASAIAASREAIW
                     GILSDPAKFSKL"
     gene            10903..11151
                     /locus_tag="AC16CX_03735"
     CDS             10903..11151
                     /locus_tag="AC16CX_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011656.1"
                     /translation="MIEHMKVTARVALMTVLFAGPSLAADELSKDLTSTIALLGLPCG
                     QVVSSKRLGENDYLATCSNKMRYRVFLNAQGRVVAQKQ"
     gene            11284..12273
                     /locus_tag="AC16CX_03740"
     CDS             11284..12273
                     /locus_tag="AC16CX_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3011657.1"
                     /translation="MPQSRDRTLVCSVLFLDIVGYSREATADQAQLKQALNRALAAAL
                     ESVPTRDRVIIDTGDGAAITFFGDPENALSVAMTIRDSLGGLPVRMGINLGPVRMVAD
                     VNDQTNIIGDGINVAQRVMSFSKPGQLLVSRSFYEVVSCLSSEYENVFAHEGARTDKH
                     VRAHDLYSVAADARIGQKAAKRKEQSPATRAADASYVEGRHARDSDEDDADQNAQIFD
                     AGPNLIVVGYSKSRVQQAVDQLAQSGSRVISPLAKVGRKWMAACEHPDVPVSACKVEK
                     LGFTHIVTGPTQEAVAVKVGELVQFGAVLVGDIEFAGGKWTAVCDASGAERHA"
     gene            complement(12344..13492)
                     /locus_tag="AC16CX_03745"
     CDS             complement(12344..13492)
                     /locus_tag="AC16CX_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011658.1"
                     /translation="MDTNIPIRPAPTPGVPLLVGITGLILVGAPLAGVWLAGRPLDEY
                     LELPPRPAPGVAAGFSWTGFWLLAAFVSAAVAPFVRRLLEAPPVKQGPVIRRKAFPFW
                     GWAALLWLAVSWTLAWTRFPWFESYQQHTFTPLWLGYIGVVNALTMRRAGSCLLTGEP
                     ARLAALFPLSALFWWFFEYLNRFVGNWHYTGIGDFDALEYTLFATLAFSTVLPAVAST
                     AEWLGTFPRLHAAFARWRSMRVPHPKLAAAPVLALTVLSLAGLGVFRELAFPLVWISP
                     VLVIICLQALGGRATVLAPLAGGDWRGVVSFAAAALLCGLFWEMWNYGSLARWEYSIP
                     YVDRFRLFEMPLLGYAGYLPFGVECAAIAALVLDQEAPQARVRPPGKN"
     gene            13635..14597
                     /locus_tag="AC16CX_03750"
     CDS             13635..14597
                     /locus_tag="AC16CX_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011659.1"
                     /translation="MKTGLLFAAAILVSIVVQNAPADKYPTKPVRLIAPFPPGGGTDI
                     LSRAIAVPLSESLGQPVVIDNRPGAGGAVGAEITARAEPDGYTLIMVSSSYTANAAYR
                     KPPYDPVNGIQPIILIGTTGLIMVVHPSVPAKSVKELIAHARANPGKLNYGSVGTGSV
                     THLAHELFKLETGVNLVHVPYKGGGPALNAVVAGEVQLTAISMVPTLPHVRAGRLRAI
                     GITTSQRSSLLPDVPAIGETVPGFEVVHWYGMWGPKGLPRDIVARWNKEVAKVLLTDE
                     MKRQTRGEGLEPGGGPPAQLGEIIRSAVERWRRVVKEAKIEREG"
     gene            14834..15724
                     /locus_tag="AC16CX_03755"
     CDS             14834..15724
                     /locus_tag="AC16CX_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011660.1"
                     /translation="MKITWTGWVLWVAALTFLMPAASGSVFPAAEPRADKMVESSNPP
                     AAGRAPAGAVPPLAYQIAAAGSIVLCVTEITGGVVSYRVREVWKASIPSFRVGESLRL
                     DTAAHELLGYRPGAEQDVVLFFVAKGLPSNQPLEVLPAVNGNVTYSPHDRSVQEKLTL
                     GQLKQRVTGGNMAPPAIIPEQGMAIPLRISFIGLKSVRWIALAHNNFSPLLVLFQDHL
                     VQRVVFRKSRNYSEIEYVDVSATDTENLDIAYTDSRFTFSCKLKTKQDLVAVLKFFGE
                     KGVKLGEGAKRLLSETGPGR"
     gene            15812..>16017
                     /locus_tag="AC16CX_03760"
     CDS             15812..>16017
                     /locus_tag="AC16CX_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3011661.1"
                     /translation="MTTLTRKLMRLLLAALVLTVPLSVMAQGEPIGIVIMHGKGGSPT
                     RLVADLARTLEGKGYLVANLEMPWS"
BASE COUNT         2980 a         5058 c         5254 g         2725 t
ORIGIN      
        1 cccggttgtc cacgatcacc tgcttgcccc attcctccgt gagccgcatg gcgaggacgc
       61 ggcccatgtt gtcgaccgag cttcccgtcc cctgcgcgac gatcaggcgc accggcttgg
      121 cgggataact cgccgctggc tcgctcgccg gagacgttcc atggctcagg ccgagcgcgg
      181 ccagcgccgc gcacgcgatc gacgacagca accgcgtacc gcgtgtcgcg gctgcatggg
      241 ctcttccgtc atcctcgttt cgatgcgtca tggcattctc cctttttatg tattgtccca
      301 gccgttgcgg cagtccggtt tgtatcataa tggaaagaag aaagcggtca taggaaaacc
      361 gggtagaacc cacgcaacgc tcgagggaga atgcgatgtc aacggagagg ctcgctgttt
      421 cactcgcgct cgcgctgctg actgcgacac ccgcccccgt gcacgcgcaa cgggaatccg
      481 ggtctcaggc ctatccgacc cggcccattc gcctcatcat tccttactcg cccggcggcg
      541 cctccgacaa catcgcgcgg ctcgtcatgc cgcggctcgg cgaggcgctc ggccagacca
      601 tcgtcatcga caaccggccg ggcggcgccg gcagcatcgg gcgcgacctc gccgccaagg
      661 cggcgccgga cgggtacact ctgctctcaa ccgacgcgcc gcacgcgatc aacgtgcacc
      721 tgcgcaaggt cccctacgac tcgctcaagg acttcacgac gatcacgacg accgccacgt
      781 cgccgatgat catgatcgtc tatccgggct ttccggcgaa gacggtgaag gaactcgttg
      841 cgatcgccag ggcgcagccg ggcaagctcc acttcggctc gggcggcacc ggcgcgatca
      901 cgcacatgac gggggagctg ttcaagctcg ccgccaatgt ggacatcgtc cacgtgccct
      961 acaagagcat cgcgcccgcg gtcacggacc tgatcggcgg acagatcccg atcgcgtttc
     1021 ccgggaccgc caccgtcggc ggccatatac gcgccggccg cgtgcgcctg ctcgcggtcg
     1081 ccgccaagca gcgctcgccc gcctttcccg aggctcccac gttcgaggaa gcgggggtgc
     1141 ccggggtggt ggccgccaac tggtacggca tcatggggcc ggcgaagctg ccgcggccga
     1201 tcgtcaaccg cctcaatgcc gagctccaca aggtcgtgcg cctgcgggaa atccaggagc
     1261 gtttcgccgc ctcggggatc gagacgctgc tgaacacacc gcaggagttc caggccatgc
     1321 tcgagtccga gaccgcgcgc tggggcgagg tagtcaaggc cgccggcgtc aagccggagt
     1381 gaatcagggg ctgccattaa atgtacgagt cgcgcgcaga cgcaccgcta tcccgtcccc
     1441 gctttttgcg gcgcctgtcc tggcatgtcg tcgctgcggt cgggctgctg ctgggatccc
     1501 tcttgctggg catgggggga tacatgtact tcgagaatct cgcctggcgc gatgcgttcc
     1561 tgaacgcttc catgctgctc ggcggcatgg ggccggtcga tgcgccaaag accaacggcg
     1621 ggaagctctt cgccggcatg tatgcgctgt attcggggct ggtcttcctg gtcgcggtgg
     1681 gcgtcgcgct ggcgccgatc gtgcatcgga tcctccacaa atttcactgg aagcagaacg
     1741 cctgagaacc cctcagggca cagaccgctt gggaaaccga tcaagccgtc tgtttaaacc
     1801 ggtagaattg ggtccgaggt aacttttcct gcgatcgctg gtctcatggt tgactgaacc
     1861 ccggtcgagc gttctgctgc ccgctagcgc aattggtaga gcgtccgcct ctgaagcgga
     1921 aggttcgcgg ttcgagcccg cggcgggctg ccacttccat taggaaataa accaggctcc
     1981 tggacgttcg ctcatcgctc ctgcggtgct aagctagacg gcgtgagcgg gcagatttcg
     2041 cccgctatcc tgtgattgca tccgaggtgg acgcggcgat gaagaagacg atgctttgtc
     2101 tctgcgtatg gggatgggcg gccgcaacgg ccgtcgcagc cgagcgttgg tcactcgagg
     2161 cctatctggg cgatgcgtac aacttccgca gcacactcaa aatcaaacaa gacggcgggt
     2221 tctcgcgctc tcttaccgcc gattacgaca cgaacgggtt cgacacgccc cagtattaca
     2281 cgctccgcgg gagccgctgg gagcaggacc gcgcgtggga gttgtccttg atccatcaca
     2341 agctgttcct gaataacccg cctgcgggcg ttgcggcgct gtccgtgagc cacggcttca
     2401 acatcctgac cgtgaaccgg gccttccgga gcggcaactg ggtctaccgt ttcggcgcgg
     2461 gcccggtcat tactcacgcg gaaggcatca tcaacggcat cagctatgac ggtccctaca
     2521 agctctcggg cgcggcgctg ctggccggag gcggccggcg tttctacgcg ggcaaatcca
     2581 cgttcctgtc gctcgaggcc atggccacgg ccgcctacgc cagcccggaa atgagcgggc
     2641 cgctcaatgc ggaaatcaag gtcaccaacg tcgccatcca cggactcgcc ggcatcggtt
     2701 tcgagttcta ggatttgcac ttagcggcgc tccgaagtcg gccgcagaga aaaacccatt
     2761 taaagtagca aaacaatcag cgagttgaac gcagttcctg cctgacagtc ctcgcgcccc
     2821 cgagccgcag ctagagttgc taccgccgga tggtttaagg tagcggatcc tcaaacagga
     2881 cttttcgcca tgaaagccgc gctgattgtc tttgaccgga tgacctactt ggacttcgtc
     2941 ggcttttacg atccggtgac gcgcctgaaa tccatgaaca tcatccccga tttcgaatgg
     3001 cggatatgct cgaacaagaa acgggttgtc gatgaccggg gggcgacggg gatgacgatg
     3061 attcccgatt cggtgatgga acccctgaac ggctatgacc tgctgttcgt gcccggggga
     3121 ctgggaactc gcgagcttca aagcgaccgc gaattcatcg aatggctcaa gaccgccgct
     3181 ccggccaggc cgaagatcag cgtatgcacg ggcgctcttt tgctgggagc ggcgggcttc
     3241 ctgcgcggca agcgggcgac gactcacccg tcggccctga aggaactgga gccgtattgc
     3301 gacgcggtgc tgaccgaccg catcgttgac gaaggagacg tgattaccgg gggcggcgtt
     3361 tcatcatcga tcgatttggg gcttcatgtg gtgcaccaac tcgccggccc cgaggctcgg
     3421 gctcgcgtcg ccaagcagat gtgttatccg taccaatgga aagatcaggt cgtgcgttga
     3481 catacgctga tgcaagatag tttttcatgc cgcacgagca cgagctggag gccgcggaac
     3541 gcgccgcgat cattgcggag tgggagcggc tgcgcgccga gccgcgcccg cccgctccgc
     3601 cgccatacgg gtgcgccact ttcctggttg ccgcggcgct gctcctgatc gtcccgcagc
     3661 tgctgaaact ggcgggttgg gaactgccgc ggccgctgga gctggtcgcg cttgtcgtcc
     3721 tggcgcttgc gatggcgggt gggttgttcg tcggcatctt cgttggcagc ggcatattcg
     3781 ggcgcgcctc gaaccgcgcc aatgccgcca ttgactggct cgaggccaac cccggctcga
     3841 gcgaccccga ggagcgccgg cggcaggcgg tgacgttgct gttcaacgcg gtggtttcag
     3901 acgggcctac cacctcgggg acgttcgacc ccgatcgggc acgggagcgg ctgggcgcaa
     3961 atctgccgta tgtcctcgca gtggagcgcg ctctggtcga agagcgcggc ctctgggtgg
     4021 tcttcggcga gaaatcaaag tgattaaacc cttcgtacaa cccgggacgg ctcagggtgt
     4081 atcctgatcg cttcagcgag tcctccgtcg ttgccgccgc ggaggacctc catactttct
     4141 gggtggggat cgtggggtat gccgtcatgg ccgcgctgtg cttttatttc ggcttcaaga
     4201 aggatgaaca atgatcaggc ccgcgctgtg tcacgccacc gccgcgattc ttgcgttaag
     4261 tctgctcgcg gcgtcagcgg cgggcgcgca aaaaaccgac tggccgacga agccggtgcg
     4321 cttcgtcgtg ccgttccctc cgggcggctc caacgatcag ctggcgcggc tgctcggcgc
     4381 gaggctcgcc gagacccacg gccagccctt cgtcgtggac aaccggcagg gcgcctccgg
     4441 ggcagtcggc accgcggtgg tggccaaggc ccctgcggat ggttacacct acttattcgc
     4501 attcgacacg cacgcgatca atccggcgct gtaccgcaac ctggcgtacg acacgttcag
     4561 ggatttcacg ccggtaatgc tcattggaac cgccccgcag tgcatcgcgg cgaactcgac
     4621 ctcccggttc aagagcatgg ccgaagtgat ccgggcggcg aaggcgaggc ccgactcggt
     4681 gagctatggt tcgaccggca acggcacgct gggccatctc atgatgatgc tgttgcagaa
     4741 ggagggcggg ttcaaggccg tgcacgttcc gtacaagggt ttcgccccga tggcgcagga
     4801 cgtgatgggc ggtcagctcg actttgggct cgggacggtc gcggtgcttt caccgttcgt
     4861 cgccagcggc cgcctgcgcg gcctggccgt ctccagcgag acgcgttcct tggcgatgcc
     4921 cgacgtgccg acgctctcgg aacagggctt ccccgggttc tcgggcctgt cgtggtgggg
     4981 aatcttcgct ccggccaaca cgcccaagcc cgtcgtagcg aagtttcatg ccgagctcgc
     5041 gaggatcatc aacgcgccgg agctgcgcaa gcgcctcagc gagcagctcg gcatgaccgt
     5101 ggtcacgagc agcccggagg ccttgcagaa attcgtggtc agcgaagcct cgcgctgggg
     5161 taaagtgatc aaggacaacc agattcgacc cgactgaacc gaagcagcgg agtcggagtc
     5221 attcttatgt tcgtgattcg tgattcgtga ttcgtgattc gtgattcgtg attcgacccg
     5281 gtttcgtcac tgcgtaactg cgtcgctacc aatagggagg aaacatgatt cgagctgcaa
     5341 tcgtcggcct gggctggtgg ggcaagacga tcgtggagtc cgttcagggc aagagcgatc
     5401 agatcacctt cgtcgccgcc aacacgcgca cgcgcgccaa agcggaggag ttctgccgcg
     5461 acaagaagat cgatctgcgc gacagcctcg acgacatcct gaaggacccg aagatcgaag
     5521 cggtggttta caccacgccc cacagccagc acgaggagca cgtcaggcgc gcggcgcagg
     5581 cgggcaagca cgtctgcatg gagaagccct tcaccctcac cgcggcgagc gccagggccg
     5641 cgatcgaggc ggtcaagaag gcgggcgtcg tgctcgccgt cgacttccag cgccggtttc
     5701 acccctccgt cgccgagatc cgcaagcgcg tgcgggacgg aagcctgggt ccgatctcct
     5761 tctgcgtcgg cgaagtgtcc acgccgagcg ggctcgccct gccgaaggag tcgtggcgcg
     5821 tgaacccgga ggaaactccg gcgggcgcga tgacggggct gggggtacac ctcgtggacg
     5881 cgttcatcga tctctgcggc gaggtggagg aggtgtattg catcaacgcc cgccgcgccg
     5941 cgccgctcgt ggatgatacg acagttttca ccatgaagca taagaacggt gtgatctcca
     6001 cgaactattg cacgctggcg agcgccgcga gctattgcat cgccgtcttc ggcaagaacg
     6061 gcatcgccga gacggcccgg ccaagcctgg acacgttccg cttcatgccg gtggccgctc
     6121 agggcggtcc ccacgcgacc gcgcagccgg aggtcatcga gaacaagggc gtcaatctgg
     6181 cgaaggcggt gatggaagcg tttgcggcgg cggtgcaggg gaaagcgccg tatccggtca
     6241 cccatgacca gatcgttcac ggcgtcgccg tgttcgaggc catcgtgaag tcggcgaaga
     6301 ccggccggcc ggtcaaagtc cgctgatacg gaatagacga gccgcacaac ccaggacagc
     6361 tcagtctgtt cccgattcat ttcatcggac ggagaggggc cgaaatgcct ttaccggtga
     6421 agtttgcggc gcttgtcttc gcagcgcata ttgccgctgc gtacgcggtc agccatgcgc
     6481 aggcctggcc gaacaagccg atccgcatgc tggtggggtt cccgccgggc ggcccgaccg
     6541 acgtggtcgc ccggatcgtg agcgaaaagg tcgggggaca gatcaaccag cgcatcgtga
     6601 tcgacaatcg cccgggcgcg gccggcaaca tcgccgtcga gatcctggcg aaggccaacc
     6661 ccgacggcca tacgctgctc tattcgtcga atgcgatcgc gctctcgccg ggcctgtact
     6721 cgaagctcgg ctacgacccg ctcaaggatc tcgcgccggt caccgagatc ggcgccggct
     6781 gcctcatttt cgtggtgcac ccctcgctcc ccgtacagtc ggtgcaggcg ttcatcgact
     6841 acgcgaaagc gcggccgggc cagttgaact acgcgtcctc ggggtccggc accagcacgc
     6901 acctcgcggc ggtgctgttc tcgcagcgca ccggcatcca gacgcagcac gttccctaca
     6961 aggggaccgc gccgtcgctg gtggacatga tcgccgggcg gacccagttc ctgatgggcg
     7021 ccgtcctcac cgccgtgccc catatcaagg agggcaggct gcgggcgctc gccgtcaccg
     7081 gatcgaagcg catcaagtcg ctgccggagg tgcgcacgct ggaggaatcg gggctcccgg
     7141 gattcgtggt gacgacctgg caaggcgtgt ttgcgcccgc gggcattcca gccgcagtgc
     7201 tcgcgaaagt gaacgcggag ttcgtcaagg cggttcacag tcccgagttg agaccgagga
     7261 tcgagcagca ggacatggat ccgaccgggc ttgcggtgaa ggcgtttacc gctgcgtact
     7321 acgccgagct gaagcgctgg accaaggtgg ccagggacgc gggcctgaag gcggattaga
     7381 aatgagcgat ccgttcgagg accactgctg gaaggacgtc atcccggccg acgtgctgga
     7441 gatttactcc tattacaggc gggaggtctc cgtcggtccg aatccggcgc tgctcgccgt
     7501 tgatctctac gagctggtgt accagggcgg tccccggccc gtcgccgagg tcgccaggac
     7561 ttatccgagc tcctgcggcg agtacgcgtg ggcggcgatc gaaccgacca ggcgcctgtt
     7621 cgcagcggcg cgcgcggcct gcattcccgt ggcgtattca accaatgaaa cgcggcccga
     7681 tagccgcgcg acgcccgtgt ggtccacgaa gcgacagcgt gtgccgatcg gccccgagct
     7741 gtatgcgatc cgccccgaat tccagccgca gccgggagac atcgtcatcc acaagatgcg
     7801 cgcgagtgtc tttttcggca cgccgctcat cgcgcatctc acgcagctcg gcgtgcagac
     7861 cgtcatcatc tgcggcgaaa gcacctcggg ttgcgtgcgc gcgacggcgg tcgatgccta
     7921 ttcccacggc ttccacgtcg tgctggtgga ggagtgctgc ttcgaccgct cgctgctgtc
     7981 acacaaggtc aatctgttcg acatgcatca caagtacgcc gacgtgatcg gcgtcgatgc
     8041 cgtcaccgcg cacctcaaaa cgataaaaaa cctcagggga cgggccgcca agcaaaatgg
     8101 ctaggccgat cggaatttca gggggaggcg aaatgaacaa aaggttacgg ataagtgtta
     8161 cggtgggcct ggctgccgcg ctcatcgccg ccgcgccgca cgcagccgcg caatccgcgg
     8221 cggcatatcc gaccaagccg gtccgactga tcgtcgcatc gggggcgggc gggggcctgg
     8281 attttgtcgc gcgccaggtc gccactccgt tgagtgaggc cgtggggcag accgtggtcg
     8341 tggacaaccg ggcgggcgcc agcgggagca tcgccgcgga actcgcctcg caggcggcgc
     8401 cggacgggta caccctgatg ttcctcagcg cgagcctggt ggtgtacggg atcgtcaaca
     8461 agacgcgcta tgacctgtac cgcgactttg ctccggtctc ccaggtcgcc gccgcgccat
     8521 acctgctcac cgtgtatccc gggctgcccg tcaagacggt gaaggagctg gtcgcgtacg
     8581 cgaaagcggc cccgtcgaag ctgaattatt cctcgacggg caatgcctcg ctggcccatc
     8641 tcgcggccga gttattcagg gtgtccaccg gcactgagct cgtgcacatc ccttacaagg
     8701 gcgtgggcgc ggcgctgcct gatatgttgt cgggcagggt gcacatgagc tttttgagca
     8761 tgggctccgt gttcagccag gtccggaccc agaagctgaa agcgattgcc atagccagcg
     8821 ccgcgcgcgc gaagatggcg cccgaaatac cgacgatgat cgagtcgggc gtgccgggat
     8881 tcgtggtcac ccaatggcac ggcatgctgg cgccccgcgg caccgcgcgg cccgtgatcg
     8941 agcgcctgca tcgggagctc gtcaaggcgg tgcagaggcc ggaagtcgta tcgcggctgg
     9001 cgctcgacgg gaccgaggga atcgcgagct cgtcggaaga atttggcaag cacctcaggt
     9061 ccgaacgcga acagtgggcg aaggtggtga agctggcgaa cattcgcggc gactgactca
     9121 aaggcgtaag gggataccaa tcactgaaca ggtaagggag gatcagccaa tatgcgttcg
     9181 ggtcttttca tcgcagccgt cctgctggcg atatcgccgg gcgccggcat tgcccaaaaa
     9241 ctgccggcgg attgcagcac ggcggcagcg ccaaagcagc cgctggagat ctcggtcagg
     9301 gggaccaagt tcacgccgaa atcggtcaag ctgcgttccg cgggcggcgt gacctacggc
     9361 gacgagcaat tcgatactta ccgcttgtcc atgaggagcg aggatgagct ctcgccaccg
     9421 ctcgaatcgg aggtgaccat cctcgtgcgg aaggggcagc gcatcgacgg cagggtgttt
     9481 cgcaaattgc cgacgaagga aaccggcaag cagccggtgc caacgaaagg cctgcccgaa
     9541 gtgcagggct ggtcgttcaa gaaccgcccg gcgaggaccg attccagcca tgtcgagcac
     9601 gtcggttccc tgcgcctcga gttcgggcag cgccagggag cctcgatcaa cggcaatatc
     9661 tatctctgcg tggcgaaggg tcagaccacg atcttcgaca gcacgccgac gaaggaggac
     9721 agctatgccg ttggaacctt tcaggcgcgc atcgaataga atcaagaatc cagagaggcg
     9781 ccgtgaagga ttggagagtc tgaccccttg gagcacaagg cagcgtcggt tgttggtgtc
     9841 atcggcttga atttaagaca acccggcccg aagccgtaac acggcagctc gccggccggg
     9901 gctattgaag taatagcact tgaagcattg ccgcaccaag ccgaattcgc cggtgagtat
     9961 cggtccccat gccgccagct tgaacacggc gccttcctca gtcctgtaag cgttacagca
    10021 acaagcgaag cttaggccgc cacatgcggg cagttttgac ccggatcaaa tgactttgaa
    10081 taaacagggg agagatcgcg cagaccgtat ggttcgatag atccagtgta acttttccgc
    10141 ggcgcgcgcg ttgaagggga aagagaggtt acacttaccc tgtttacgat gcctgccgcg
    10201 cgcttttttg gactattttt cctcgtgctg acggcaggtt gtgccggaat cgggtgcgcg
    10261 ccaccgcgcg agccgcccca gaccggcgcg acacagggcg gggccgctac aatcgagccg
    10321 cctcaagccg gcgcgccgca gaaagcggcc accgcggtca agccgctgcc ctcgaccgcc
    10381 gtgacgcagg gcgaggccgt tgcggtcaag ccggaagccc gtccggcgac gccgtccacg
    10441 accgggccta cgggcgagag cgtcgccaag aacgacgcgc cgggcgccaa aacccccgcc
    10501 aaagttcccg ctactgccgc gcccaaggag ccgctaccga aaaaggagag cgccgctcca
    10561 gcgccggcga agccggcggc gccaccgtcg ctggacctga cgtcgctcga gaagcggctg
    10621 cgggagaccg acgcaatcgg cgtgatgacc aaactcacgc tcaagaacca ggtcgatgac
    10681 ctgctgaatc gattcagggc ctactatgag gggcggatca aaaccaccct cgcccagttg
    10741 cggcagccgt acgatacgct gatcctcaag gtaatttcct tgctgcagga caaggacccc
    10801 tctctggcca gtgcgatcgc ggcgtcgcgc gaggcgatct ggggcatact ttcggatccg
    10861 gcgaagtttt cgaaactgtg aactgacgac gggagaagcg ctatgatcga acatatgaaa
    10921 gttacggcgc gggtcgcttt gatgacggtg ttgtttgccg ggccgtcgct cgccgcggac
    10981 gagctttcca aggatctcac gtccacgatc gctctcctgg gcctgccgtg tggccaggtc
    11041 gtcagctcga agcggctcgg cgagaacgac taccttgcaa catgctcgaa caagatgcgc
    11101 taccgggtgt ttctgaacgc ccagggccgg gtcgtcgcgc agaaacaata ggaagaccgg
    11161 gcgaaattcg acccgagtcc ttccgccttg gctccggcct cccgggcgcc gtagaattcc
    11221 aatcacggca cccatgatgc cccggcctat caggggcata tttatcgatc tggagcggga
    11281 agaatgccgc aaagccgaga tcgcacgctc gtatgcagcg tgctgttcct cgacatcgtg
    11341 ggatactcga gggaggcgac cgccgaccag gcgcagctca agcaggcgct taaccgcgcg
    11401 ctggccgctg cgctcgagtc cgtgccgacg cgcgaccgcg tcatcatcga cacaggcgac
    11461 ggcgccgcga tcaccttttt cggagacccc gagaacgcgc tgtccgtcgc catgacaatc
    11521 cgcgattcgc tcggcgggct gccggtcagg atggggatca acctgggccc ggtacggatg
    11581 gttgccgacg tcaacgacca gacgaatatc atcggcgacg gcatcaacgt cgcccagcgc
    11641 gtgatgagct tctcgaagcc cggccagttg ctggtgtcgc gctcgttcta cgaggtggtg
    11701 tcgtgcctgt cgagcgaata cgaaaacgtg ttcgcgcacg agggggcgcg caccgacaag
    11761 cacgtgcgcg cgcacgacct gtactcagtc gccgcggacg cgcgcatcgg gcagaaagcg
    11821 gcgaagcgaa aggagcaaag ccccgccacc cgggctgccg atgcctccta cgtggaaggc
    11881 cggcacgcgc gtgattccga cgaggatgac gcggaccaga acgcccagat cttcgatgcg
    11941 ggacccaacc tgatcgtggt cgggtacagc aagtcgcgcg tccagcaggc ggtggaccag
    12001 ctggcgcaaa gcggctcccg cgtgatttcg ccgcttgcca aggtcggcag gaaatggatg
    12061 gcggcctgcg agcacccgga cgtgcccgtg agcgcctgca aagtggagaa attgggattc
    12121 acgcacatcg tgaccggccc gacgcaggaa gccgtcgccg tgaaggtcgg ggaattggtg
    12181 cagttcggcg ccgttctggt cggcgacatc gaattcgccg gcggcaagtg gaccgccgtt
    12241 tgtgacgcct ccggcgcgga gcgtcacgcg taaagccggt cggacggcct gttacgcatt
    12301 gccggcgtcg ctttccggat tcccccggaa taatgactcg ggctcagttt ttcccgggcg
    12361 ggcggacccg ggcctggggc gcctcctgat ccagcaccaa cgcggcgatc gccgcgcact
    12421 cgacgccgaa gggcagataa cccgcgtagc ccaacagcgg catctcgaag aggcggaacc
    12481 ggtccacgta cgggatgctg tattcccacc gggcaaggct gccgtagttc cacatctccc
    12541 agaacaagcc gcacagcagc gcggccgccg caaagctcac gacgccgcgc cagtcgccgc
    12601 ccgccagcgg cgcgagcacc gtcgcccggc cgccgagcgc ctgcaggcag atgatgacca
    12661 gcaccggcga gatccatacc agcgggaacg cgagctcgcg gaagacgccg agcccggcga
    12721 gcgagaggac ggtgagggcg agcacggggg cggcggcgag cttcgggtga gggacgcgca
    12781 tcgagcgcca gcgcgcgaag gcggcgtgaa gccgcgggaa tgtgccgagc cattccgccg
    12841 tgctcgctac cgccggcagc acggtggaaa aggcgagcgt cgcgaagagc gtgtactcga
    12901 gcgcatcgaa gtccccgatg cccgtgtagt gccagttgcc gacgaagcgg ttcaggtact
    12961 cgaagaacca ccagaagagc gcgctcaacg gaaacagcgc ggcgagacgc gcaggttccc
    13021 cggtcagcag gcagcttccc gcgcgccgca tcgtgagggc gttgacgacg ccgatataac
    13081 cgagccagag cggcgtgaag gtatgctgct ggtaagactc gaaccaggga aagcgcgtcc
    13141 aggcgagcgt ccagctcaca gcgagccata gcagggccgc ccagccccag aacgggaaag
    13201 ccttccgcct gatcaccggg ccttgcttca cgggtggagc ttccaggagc cgccggacaa
    13261 acggcgcgac ggccgccgag acgaacgccg caagcagcca gaagcctgtc catgagaatc
    13321 cagccgctac cccgggcgcg ggccgtggcg gaagttccag gtactcgtca agcggtcgcc
    13381 ccgccagcca cacccccgcg agcggggcgc cgacgagtat caatcccgtg attcccacca
    13441 gcagcggcac gcccggcgtg ggcgcgggcc tgatcggaat gtttgtatcc attcttgccg
    13501 tgccccgtct atcgcgcaag aatacgccgg gaaagatctt gattgtaaga tttgagtcgg
    13561 aacagaaaac tgataattca ggagctgcca ctcgtgccgt gatgcagtcg tgcttgcgcg
    13621 aggagggaaa gaaaatgaaa accggactgt tgtttgctgc cgccattctt gtttccatcg
    13681 ttgtgcagaa cgcgccggcc gataagtatc cgaccaagcc ggtgcgcttg atcgcgccct
    13741 ttccgccggg cggcgggacg gacatcctgt cgcgggcaat cgccgtgccg ctgtccgagt
    13801 cgctcgggca gccggtcgtg atcgacaacc ggccgggcgc tggcggcgcc gttggcgccg
    13861 agatcaccgc gcgcgccgaa cccgacggct acacgctgat catggtttcc agcagctaca
    13921 ctgccaacgc cgcgtatcgc aagccgccat acgaccccgt taacggcatc cagccgatca
    13981 tcctgatcgg cacgacgggg ttgatcatgg tggtgcatcc ctcggtcccg gcaaagagcg
    14041 tcaaggagct catcgcccac gcgagggcca accccggcaa gctcaactac ggctcggtcg
    14101 gcaccggcag cgtcacccac cttgcacacg agctgttcaa gctggagacc ggggtgaatc
    14161 tcgttcacgt tccttacaag ggcggcggcc cggcgttgaa cgcggttgtt gccggagagg
    14221 tgcagctcac cgccatcagc atggttccaa ccctcccgca cgtgagggcg gggcgcctgc
    14281 gcgccatcgg aatcaccacg tcccaacgct cgagcttgct gcccgacgtg ccggccatcg
    14341 gcgagacggt gcccggcttc gaggtcgttc attggtacgg catgtggggt ccgaaaggcc
    14401 tgcccaggga catcgtcgcg cggtggaaca aggaggttgc gaaagtactg ctcacggacg
    14461 agatgaagcg ccagacgaga ggggaaggac tggagcccgg cggcggaccc ccggcgcaac
    14521 tcggggagat catccggagc gccgtcgaaa gatggagaag agtggtcaag gaggcgaaga
    14581 tcgagcgcga aggctgatcc gagccaacct gggctagccc aacgcgctcc atccaggcaa
    14641 gctggcccgg agtcactttg tcggcgaacc gaatgaactt cgcgcgcgag cgcggagagt
    14701 ggctctcgga cgattgagag caagccatcc ttgccgcggt ttgcgttctg tttggccccg
    14761 gtatttgcat catcggcatc ggcctcgaac cgggagtgct gaaccggcgc tcgagagccg
    14821 accggcgttg agagtgaaga tcacgtggac aggatgggtg ttgtgggttg ccgctctgac
    14881 attcctcatg ccggcggcca gcgggagcgt tttccccgcg gcggagcccc gggccgacaa
    14941 gatggttgaa tcttccaacc cgccggcggc cggccgcgcc ccggctggcg ccgtgccgcc
    15001 gctggcgtac cagatcgccg cagccggaag catcgtgctg tgcgtgacgg aaatcaccgg
    15061 cggggtggta tcgtatcggg tgcgggaggt ctggaaggcc tcgattccgt ccttccgggt
    15121 gggcgaatcg ttgcgcctcg acacggccgc gcatgagttg ctcggttacc gtcccggcgc
    15181 ggagcaggat gtggtcctgt tctttgtcgc caagggcttg ccgtccaacc agccgcttga
    15241 agtcttgccg gccgtcaacg gcaacgtgac ttattcgcct cacgaccggt cggtgcagga
    15301 aaagctcacg ctgggccagc tcaagcaacg ggtcaccgga ggaaacatgg cgccgccagc
    15361 aatcatccct gagcagggca tggcaatccc cttgaggatc tcgttcatcg ggctgaaatc
    15421 ggtgcggtgg attgccctgg cgcacaacaa tttctcgccc ttgctggtgc tgttccagga
    15481 ccatctcgtg cagagggtgg tcttcaggaa atcaagaaac tactccgaaa tcgagtatgt
    15541 ggacgtgtct gcaaccgata ccgagaatct cgatattgct tataccgaca gccggttcac
    15601 cttcagctgc aagctcaaga ccaagcagga cttggttgcc gtgctgaaat tcttcggcga
    15661 gaagggcgtc aagctgggtg agggcgcgaa gcggttgttg agcgaaaccg gccccgggcg
    15721 ctagcgtcgc agtggggacg aagcccggtt tcccgccgtc gcaggataaa atgccggctg
    15781 cccgatcggg cccgaaccag ggagagaaag catgacgaca ttgactcgaa agctgatgcg
    15841 gctcctactg gcggcgctcg tcctcaccgt gccgctttcg gtgatggccc agggggagcc
    15901 gatcggcatc gtgatcatgc acggcaaggg cggatcgccg acccggctcg ttgccgatct
    15961 cgcccggacg ctggagggga agggatatct cgtcgccaac ctggagatgc cctggtc
//
LOCUS       DBNGWZ010000123         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1019377, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000123 DBNGWZ010000000
VERSION     DBNGWZ010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1019377"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16CX_03765"
     CDS             <1..246
                     /locus_tag="AC16CX_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011662.1"
                     /translation="GAAAQATPAAPAKGEPAKATPATPAQPGKPTAEKAKTEKKAKTE
                     KKKAEPKKAPAKPKGEAKKGEGKKSEGEAMKKGQEGK"
     gene            336..1472
                     /locus_tag="AC16CX_03770"
     CDS             336..1472
                     /locus_tag="AC16CX_03770"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3011663.1"
                     /translation="MHYIDSKTGATYSLDDPRWRGDSGGHVNLGDAPGLRRNEIDASI
                     RSLWRYRRALLVDAGDAVTMGEGWTPLVRAQWDGLPVSMKLEYLMPTGSFKDRGTTVM
                     ISYLKSRGVTSVLEDSSGNGGASIAAYAAAAGMRCRILVPETTSYPKIVQMAATGADV
                     VVVRGTRQQVAEEALRQAETLFYASHNWQPFFVEGTKTLAYELWEQLEFHTPDNVIVP
                     LGYGSNVLGCGRGFGELKRRGEIARLPRIFGVQAANCAPYHAAFTAGAESLVPIEVKP
                     TVAEGVASSKPVRVGETLAAIRDSGGSVIAVSEEEIAAALGGLARMGFYVEPTSATAA
                     AGLTRLLKQATIGRDEATVLVLTGSGLKASDTIGKLLNLKARQG"
     gene            complement(1534..>1789)
                     /locus_tag="AC16CX_03775"
     CDS             complement(1534..>1789)
                     /locus_tag="AC16CX_03775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3011664.1"
                     /translation="VELAPHLKVPVLGLYGGADASIPVEHVEQMRAALKSTDNTVSEI
                     VVYDGAPHAFYADYRPNYRKEAAEDGWKRMQAWFKKYGVA"
BASE COUNT          341 a          586 c          607 g          255 t
ORIGIN      
        1 ggtgccgcgg cgcaggctac gcccgcggcc ccggccaagg gcgaaccggc gaaggcgacc
       61 ccggcgacac cggcccagcc cggcaagccg acggccgaga aggccaagac ggagaagaag
      121 gccaagaccg agaagaagaa ggctgaaccc aagaaggccc cggccaagcc caagggcgaa
      181 gccaagaagg gcgaaggcaa gaagagcgag ggcgaggcca tgaagaaggg ccaggaagga
      241 aagtaacaaa gacgttgccg ctcgcgcgat caacgcgcgg cgcggaccgg gaaaaaggtg
      301 ggacatcacc cgcctttttc ttttagcatt ccgaaatgca ttacatcgac tcgaagaccg
      361 gcgcgaccta ttccctcgac gatccgcgct ggcgcggcga ttccggcggc cacgtcaatc
      421 tgggggacgc gccgggcctc aggcgcaacg agattgacgc ctcgatccgc tcgctgtggc
      481 gctaccggcg ggcgctgctg gtggatgccg gagacgcggt gacgatgggt gagggctgga
      541 cgccgctcgt ccgcgcgcag tgggacggcc tgccagtctc gatgaagctc gagtatctga
      601 tgccgaccgg ctcgttcaag gaccgcggca ccaccgtgat gatctcctat ctcaagagcc
      661 gtggggtgac gagcgtgctc gaggattcct ccggcaacgg cggggcctcg atcgctgcct
      721 acgccgccgc cgccggcatg cgctgccgca tcctggttcc ggagacgacg tcttacccca
      781 agatcgtgca gatggccgcg acgggcgcgg acgtggtcgt cgtcaggggc acgcgccagc
      841 aggtggcgga agaagcgctg agacaggccg agacgctgtt ctacgcaagc cacaactggc
      901 agcccttctt cgtcgaaggc acgaaaaccc tcgcctacga gttgtgggag caactggaat
      961 tccacacgcc cgacaacgtc atcgtaccgc tcggttacgg cagcaacgtg ctcggctgcg
     1021 gccgcggctt cggggaactc aagcgccgcg gagagatcgc gcgtctgccg cgcatcttcg
     1081 gcgtgcaggc cgccaactgc gcgccgtacc acgcagcgtt cacggccgga gccgagtcgc
     1141 tcgtgcccat cgaagtgaag cccaccgtcg cggaaggcgt tgcctccagc aagccggtgc
     1201 gcgtgggcga gacgctggcg gcgatccgcg actcgggtgg aagcgtcatt gcggtgagcg
     1261 aggaagagat cgccgcggcg ctgggcgggc tcgcccggat ggggttctac gtcgagccga
     1321 cctcggccac ggcggcggcg ggcctcaccc ggctcctcaa acaggccacg atcggacgcg
     1381 acgaagccac cgtgctggta ctcaccggct ccggcctgaa ggcatccgat acgatcggca
     1441 agcttctgaa cctaaaggcg cgtcagggtt agccagcgat gaatcgcgtc agacggagtt
     1501 agagctcgag tcacgagtca ccagtcacgg tagtcaggcg acgccgtatt tcttgaacca
     1561 cgcctgcatc cgtttccagc cgtcctccgc cgcctccttg cggtaattcg gccggtaatc
     1621 ggcgtagaag gcgtgcggcg cgccgtcgta gacgacgatc tcgctcacag tgttgtccgt
     1681 gctcttcagc gcggcgcgca tctgctcgac gtgctcgacc gggatgctcg cgtccgcccc
     1741 gccgtacaaa cccagcaccg gcaccttgag atgcggcgcg agctccacg
//
LOCUS       DBNGWZ010000124         3577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2783138, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000124 DBNGWZ010000000
VERSION     DBNGWZ010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2783138"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16CX_03780"
     CDS             complement(<1..479)
                     /locus_tag="AC16CX_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3011665.1"
                     /translation="MSADIHVLSAGAIEPGLIAAVEAFRKKTRRNVRITWATTPAIRK
                     RIGEGDAADVLIVPPEAVDDFARQGKVAGQERVYLGRVGVGVVIREGAPVPDVSSVDA
                     LKRAVLDAESVVFNRASSGLYVERLLGELGLLERIQDRTTRCGNGPEMMEHLINGKGR
                     "
     gene            complement(476..1840)
                     /locus_tag="AC16CX_03785"
     CDS             complement(476..1840)
                     /locus_tag="AC16CX_03785"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011666.1"
                     /translation="MTVLTAELGRFLENIQFDHLPASTLPLVRNAYTDTVGVIMVGIK
                     EPVVDIVRRTLVETGGRREARACLSPIQVSAPDAALLGGTAAHALDYDDHSLTGHPSA
                     VLVPAILAEGEALGSSGRDLVTAYVAGYEVWAELIRRDTNHHKKGWHPTAIFGVVAAA
                     AAAATLHRLPAERAAAALAIAASHASGLAPNFGTMTKPYHAGLAARNGLSSARLAAAG
                     MTAGRDTLENAQGFLNAFSPSSADRDSPVKVGKEWYLPWQSLCVKKYASCYFTHRSFD
                     TAVKMLAGRKLGPDDIAGIEVTMGLGQVVVLANHSPQTGLEAKFSEEFAMAAAVILGR
                     MSVDVFTDEIVRRRDVQAFFPKVKLIAVDEHDTRDPSHSPTERVVIRLKNGEVLDSGE
                     ITRIRGHAYDPMSPEELWTKFAECTAKTHPEKEARQLFAMLQAVDELPSARDLPTCKT
                     IFAA"
     gene            complement(1935..2900)
                     /locus_tag="AC16CX_03790"
     CDS             complement(1935..2900)
                     /locus_tag="AC16CX_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011667.1"
                     /translation="MRLLAKTVWLLTLALAAAGAVAQSFPTKPIRILIGFPPGGGIDI
                     VARLLAPRLSESLGQPVVVDNRAGANGIIATEIVAKAAPDGHSIFFGTTGNLSVNPAL
                     FPKLPFNVERDFAPLTQTSSVPFLIYVHPSVPVKTLAELIAHAKANPGKLHFYSSGNG
                     GLPHLAGELLKSVAAINTVHVPYKGSAPGMSDLIGGHVQFGFDAVAIGLPHVKAGRLR
                     AVAATGPKRLSFLQDVPSASETLPGFEVVNWYGMVVPAGTPRAVIERLRAEIVKAMNI
                     PDIREKLIAQATDPVGSTPKEFGAFRKAEEAKWARVIKEANIRPD"
     gene            3087..>3577
                     /locus_tag="AC16CX_03795"
     CDS             3087..>3577
                     /locus_tag="AC16CX_03795"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase/maleate isomerase family
                     protein"
                     /protein_id="HIR3011668.1"
                     /translation="MTAALKVGLMVPINNTTFERELLAWLPAGSACHTLRIPRGKGLL
                     TTETLPAYKAQALSLAARFANLDIDVVAYGCTAAGFISGPAGDARLARELAEITGKPV
                     VTTARSMVLALQESNARDIALVTPYLDTVNEQLKAFLADGGIRVRRFNSFYAANVDEL
                     GRIR"
BASE COUNT          623 a         1175 c         1129 g          650 t
ORIGIN      
        1 cggcccttcc cattgatcag atgttccatc atctccggcc cgttaccgca gcgggtcgtc
       61 ctgtcctgga ttcgctcgag cagccccagt tccccgagca gcctctcgac gtaaagcccg
      121 ctcgaagccc ggttgaagac cacggattcg gcatcgagca cagcgcgctt gagcgcgtcc
      181 acgctcgaaa catccggcac cggcgcaccc tcgcggatca cgacccccac accgacgcgc
      241 cccaggtata cgcgttcctg accggcgact tttccctgac gggcgaaatc atcgaccgcc
      301 tcgggcggca cgatcagaac gtcggctgcg tcgccctcgc cgatgcgttt ccggatcgcg
      361 ggcgtcgtcg cccacgtaat cctgacgttc cgtcgcgttt tcttgcggaa tgcttcgacg
      421 gccgcgatca atccgggctc gatcgccccg gcgctgagca catgaatatc ggcactcacg
      481 cggcaaagat cgtcttgcag gtgggcaaat cgcgagccga tggcagctcg tcgactgcct
      541 gcagcatcgc gaatagttgc cgcgcttctt tctcgggatg ggttttcgcc gtgcattcgg
      601 cgaacttggt ccagagttct tccgggctca tgggatcata ggcgtggccc cggatgcgcg
      661 tgatttcccc gctgtccagg acctcgccgt tcttgagccg gatcacgacc cgctcggtcg
      721 gcgagtgtga cgggtccctc gtgtcgtgct cgtccaccgc gatcagcttc accttcggga
      781 agaacgcctg gacgtcccgg cgccgcacga tctcatcggt gaaaacgtcc acgctcatgc
      841 ggccgagtat gacggcggct gccatcgcga attcctcgct gaacttggcc tcgaggccgg
      901 tctgcgggct gtggttggcg agcacgacga cctgccccag tcccatggtg acctcgatgc
      961 cggcgatatc gtccgggccc agcttgcgcc ctgccagcat ctttaccgcg gtatcgaagg
     1021 agcggtgcgt gaagtagcag gacgcgtatt tcttgacgca tagcgattgc cagggaaggt
     1081 accactcctt gccgactttg accggcgagt cgcgatcggc gctggacggc gagaacgcat
     1141 tgaggaatcc ttgcgcgttc tccagggtgt cgcgccccgc cgtcatcccg gcggcggcca
     1201 agcgcgccga cgacaatcca ttgcgggcgg ccagcccggc atggtacggc ttggtcatgg
     1261 ttccaaagtt cggcgccagg ccgctggcgt ggcttgccgc aatcgccaag gcagcggcgg
     1321 cccgctcggc gggaagacga tgcaatgtcg ccgccgccgc ggcagcggcg accacgccga
     1381 aaatcgcggt cgggtgccaa cccttcttgt ggtgattggt gtcgcgccgg atcagctcgg
     1441 cccagacctc gtatccggcg acgtaggccg tgacgagatc gcgaccactc gaccccaggg
     1501 cctcgccctc ggccaggatg gccggcacca gcaccgcgct cggatgcccg gtgagggagt
     1561 ggtcgtcgta atcgagtgca tgcgccgcgg ttccgcccag cagcgctgcg tcgggcgccg
     1621 aaacttggat cggggaaaga caggcgcgcg cttcgcgccg ccctcccgtt tcgaccagcg
     1681 tccggcgcac gatatcgacg acgggctcct tgatgccgac catgatcacg ccgaccgtgt
     1741 cggtgtacgc gttcctcacg agcggcaatg tgcttgccgg cagatggtca aactggatgt
     1801 tctcgaggaa ccggcccagt tcggccgtca atacggtcat catcaaactc cttggctgcg
     1861 ggtcggagtc cggaacgaca ccggcaggat ccggaatttt gcgcgtcgct tccgccggcc
     1921 gagatccggg acgatcagtc gggacggata ttggcttcct tgatcacgcg cgcccacttg
     1981 gcctcctcag ccttgcggaa ggcgccaaac tccttgggcg tgctgccaac cgggtcggtg
     2041 gcctgggcga tcagcttctc gcggatgtca ggaatgttca tggccttgac gatctcggca
     2101 cgcagccgtt cgatgacggc acgcggcgtg cccgccggca ccaccatgcc gtaccagttc
     2161 acgacctcga agccgggcag tgtttcactg gctgacggaa cgtcctgcag gaaggacagc
     2221 cgtttgggcc ccgtggcggc gacagcgcgc aacctgccag ctttcacgtg cggcagcccg
     2281 atcgcaaccg cgtcaaagcc aaactgcaca tgaccgccga tgaggtcgct catcccggga
     2341 gcactgccct tgtagggaac gtggacggta ttgatggcgg caacggactt cagcagctcc
     2401 ccggcaagat gcggaaggcc gccgttgccg ctcgagtaaa agtggagctt gccggggttc
     2461 gctttggcgt gggcaatcag ctccgcgagc gtctttaccg ggacggaagg atggacgtag
     2521 atgaggaacg gcacagacga tgtttgggtc aagggtgcga agtcgcgctc aacgttgaaa
     2581 ggcagcttcg ggaagagcgc cggattgacg ctgaggttgc ccgtcgtgcc gaaaaagatg
     2641 ctgtggccgt cgggcgcagc cttggccacg atctcggtgg caatgatgcc gttcgcgccg
     2701 gcgcggttat ccacgaccac cggctgcccc aggctctcgg agagcctggg cgcgagcagc
     2761 cgcgcaacga tgtcgatgcc gccgcccggc gggaaaccaa tgagaattcg gatgggtttg
     2821 gtgggaaacg attgtgccac cgcgccagcg gcagccagtg cgagcgtgag cagccatacg
     2881 gtcttggcaa gcagtcgcat gatcgttctt ccggcagcag ctggtttgac aatttgtttc
     2941 aaatagtagg agaatttaaa acactcgtca atgcgggcat caccctcccc atgggccaag
     3001 ctcgtgccgg gaaccggcaa taagtcaggc gtgacaatcg cttggccgcg cattaagctc
     3061 ctatttcttc catggagtcc cggaacatga cagcagcact caaggtcggc ctgatggttc
     3121 ccatcaacaa cacgaccttc gagcgtgagc tgctcgcttg gctgcccgcg ggatcggcct
     3181 gtcacacgtt gcgcatcccg cgcggcaagg gcctgctcac gacggaaacc ctgcccgcct
     3241 acaaggcgca ggcgctctcg ctcgccgccc ggttcgccaa tctcgatatc gacgtcgtgg
     3301 cctacggctg caccgcggcc ggatttattt ccggacccgc cggcgacgcc cggctcgcgc
     3361 gcgagctcgc cgaaatcacc ggcaagccgg tggtcaccac ggcgcgatcc atggtccttg
     3421 cattgcagga gtccaatgcc agggacatcg cgctcgtcac gccgtacctg gacacggtca
     3481 acgagcaatt gaaagccttt ctcgccgatg gcggcatccg cgtgcggcgc ttcaacagct
     3541 tctacgccgc caacgtcgac gaactgggcc gcatccg
//
LOCUS       DBNGWZ010000125         2326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3286612, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000125 DBNGWZ010000000
VERSION     DBNGWZ010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3286612"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC16CX_03800"
     CDS             complement(<1..633)
                     /locus_tag="AC16CX_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR3011669.1"
                     /translation="MKLNDTGLALPPQQVTLDVLLEKYAKGDEKTVEAVRRRVARALA
                     AVEKEPARWESVFLEALENGFIPGGRVNSAAGTPLQATLINCFVQPVGDSVSETVDGK
                     PGIYVALMEAAETMRRGGGVGYDFSAIRPKGARVRGTDSAASGPVSYMRVFDKSCETV
                     ESAGSRRGAQMGILRCDHPDIEDFIHAKDEGELKNFNVSVAVTDAFMRAVE"
     gene            complement(838..2310)
                     /locus_tag="AC16CX_03805"
     CDS             complement(838..2310)
                     /locus_tag="AC16CX_03805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3011670.1"
                     /translation="MEDSRLPGPILQEIVISNSFENLGLSAELLRAVAEQGYTEPTPI
                     QAQAIPVVLSGRDLLGAAQTGTGKTAGFALPILQRLSGQANISASPARHPVRALILTP
                     TRELAAQVQESVRAYGRHVKLRSTVIFGGVGFNPQATELRRGVDIVVATPGRLLDHVE
                     QRTIDLRHVEILVLDEADRMLDMGFIPAIRRILALLPSRRQNLLFSATFPDEIRKLAG
                     SFMRDPATIEVAPRNKPVELISQVAHPVDSGRKRELLSHLVKTNNWQQVLVFTRTKHG
                     ANRLAQQLERDGIEADAIHGNKSQPARTRALKRFKDNELQVLVATDIASRGLDIEELP
                     HVVNYDLPHVSEDYVHRIGRTGRAGLSGEAVSLVCGEDRPLLGAIERLTGRRIEQRVV
                     PGFEPGSAFHAPATRHEQQHHRQERGRQHAAQPRHGHGGNRKQNNRQEHRHEPLTPAH
                     GQRSAPQPQRAQRDGGQPSPQRAQTQPFPALFGTYGRKAG"
BASE COUNT          337 a          765 c          813 g          411 t
ORIGIN      
        1 ctcgaccgcg cgcatgaagg cgtcggtcac cgccacgctc acgttgaagt tcttcagctc
       61 cccctcgtcc ttggcgtgga tgaagtcctc gatgtcgggg tggtcgcagc gcagtatccc
      121 catctgcgcg ccgcggcgcg agccggcgga ctccaccgtc tcgcagctct tgtcgaacac
      181 gcgcatgtac gataccggcc cgctcgcggc gctatcggtg ccgcgcacgc gcgcgccctt
      241 cggccggatc gcggagaagt cgtagcccac gccgccgccg cggcgcatgg tttccgcagc
      301 ctccatcagc gcgacgtaga tgccgggctt gccgtccacg gtctcggaga cggagtcgcc
      361 gaccggctgc acgaagcagt tgatgagcgt ggcctggagc ggcgtgcccg cggcggagtt
      421 gacgcgcccg ccggggatga agccgttttc gagcgcctcc aggaacaccg actcccaacg
      481 cgccggctcc ttctcgaccg cggccagcgc gcgcgcgacg cgccggcgca cggcctcgac
      541 cgtcttttca tcgcccttgg cgtatttctc cagcagtacg tcgagcgtga cttgctgcgg
      601 cggcagtgcg aggccggtat cgttgagctt catgttccct tccggaaatt ttatattttt
      661 tacccggccg ggtgccggca gcagtcatga tacgccggtt gaggagcggt ttctaaccgc
      721 ttgtttgaat tagtttgacg aaaatcaaaa cgcaccagaa ctccccggtc cgaagggaca
      781 ggccacgggt agggtgaatt cctgctacgg gagccgcgcg gctcccgggt gccgccctta
      841 acccgctttg cggccgtagg tcccgaacag cgccgggaag ggctgcgtct gagcgcgctg
      901 aggagacggc tgcccgccat cgcgctgggc gcgctgtggc tgtggtgcgg aacgttgccc
      961 gtgggcggga gtaagcggct cgtgccggtg ctcctgccgg ttgttctgct tgcgattgcc
     1021 gccgtgcccg tggcgcggct gcgcggcatg ttgccgcccg cgctcctggc ggtgatgctg
     1081 ctgctcgtgg cgggttgccg gggcgtgaaa cgctgatccg ggctcgaagc cgggcacgac
     1141 ccgctgctcg atgcgccggc cggtcaggcg ctcgatcgcg ccgagcagcg gccggtcctc
     1201 gccgcacacc agcgacaccg cctcgcccga taagcccgcg cggccggtgc ggccgatgcg
     1261 gtgcacgtag tcctcggaaa cgtgcggcag gtcgtagttc accacgtgcg gcagctcttc
     1321 gatgtccagc ccgcgcgatg cgatgtcggt ggcgaccagc acctgcagct cgttgtcctt
     1381 gaagcgcttc agcgcgcgcg tgcgcgccgg ctggctcttg ttgccgtgga tcgcgtcggc
     1441 ctcgatgccg tcgcgctcga gctgctgggc gagccggttc gcgccgtgct tggtccgggt
     1501 gaacaccagc acctgttgcc agttattggt cttgacgagg tgcgagagca gctcgcgctt
     1561 ccgccccgaa tccacgggat gcgcgacttg cgaaatgagc tcgaccggct tgttgcgcgg
     1621 cgccacctcg attgtcgccg ggtcgcgcat gaacgagccc gcgagcttgc ggatctcgtc
     1681 cgggaatgtc gcggagaaca gcaggttctg acgcctggac ggaagcaggg cgaggatgcg
     1741 gcggatggcc gggatgaagc ccatgtcgag catgcggtcg gcctcgtcca gcaccaggat
     1801 ttcaacgtgg cgcaggtcga tcgtgcgctg ctcgacgtgg tcgagcagcc gccccggcgt
     1861 cgcgacgacg atgtccacgc cgcggcgcag ttccgtggct tgcgggttga agccgacgcc
     1921 gccgaagatc acggtcgagc gcagcttcac atgcctgccg taggcgcgca cgctctcctg
     1981 cacctgcgcg gcgagctcgc gggtcggcgt gaggataagc gcccggacag gatgccgcgc
     2041 gggcgatgca ctgatattgg cttgccccga cagccgctgc agtatcggga gcgcgaaacc
     2101 ggcggtcttg ccggtgccgg tctgcgcggc gccgagcagg tcgcgcccgg agagcacgac
     2161 cgggatggcc tgcgcctgga tgggggtggg ttcggtgtaa ccctgctcgg cgacagcgcg
     2221 gagcagctcg gccgacaggc cgagattttc gaatgaatta ctaatgacaa tctcctgaag
     2281 tatcggcccg ggaagacgcg agtcttccaa accggttcag gcagat
//
LOCUS       DBNGWZ010000126         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2027330, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000126 DBNGWZ010000000
VERSION     DBNGWZ010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2027330"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1464)
                     /locus_tag="AC16CX_03810"
     CDS             complement(7..1464)
                     /locus_tag="AC16CX_03810"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3011671.1"
                     /translation="MAFHTERWQKHFDGSFPILFNKVRGKPHARAVTNLFSDMRVVDH
                     MFGFDGPRDRTRRIAHALNRPLKPVVIEQKDAPCQERVILDDLDVNKWITAIRHTALE
                     PELTVGSGIRCVTGAYFDGGSDLGYNRMNFRWGNIGTFQISPGSHMWQVYTAHYDDDR
                     PIPLTMCFGVPPACTLLAGAGFDYVILPKGCDEIGVAGAVQGSPVRLVKARTVDAYAL
                     ADSEYVLEGHIHPRDKRYETSEAEKAGVQGKTFFHPEWAGYMGKAYKAPTFHVTAITM
                     RKPESRPIIFPLGVHTMDDNNIDTTVREAAIFELCNRLQPGIVQDVHIPYCMTDWGGC
                     IIQVKKRNRIEEGWQRNFLSAILSCSQGMRLAIAVSEDVDIYSMDDIMWCLTTRVNPK
                     TDILNPIPGGRGQTFMPAERMTAGEKAWTASNTLFEGGMGIDATVPYGYEKDFHRPVY
                     PVDRVKPEDFFDARDIARMKARMEGWVLSLARTGR"
     gene            1494..>1678
                     /locus_tag="AC16CX_03815"
     CDS             1494..>1678
                     /locus_tag="AC16CX_03815"
                     /inference="COORDINATES: protein motif:HMM:NF042826.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HIR3011672.1"
                     /translation="MAAGQEVVEQAYREVGRADAYQKDTVRYVAGDQMAFASTVNGML
                     MRERPAANVFMGAFFAES"
BASE COUNT          282 a          533 c          538 g          325 t
ORIGIN      
        1 tccgcgtcag cgtccggtgc gcgccagcga caacacccag ccctccatgc gtgctttcat
       61 ccgggcgatg tcgcgcgcat cgaagaagtc ctccggcttg acgcggtcga ccggatacac
      121 cggccgatga aaatccttct cgtagccgta gggcaccgtc gcatcgatgc ccatgccgcc
      181 ctcgaacagc gtgttggacg cggtccacgc cttttctccc gccgtcatcc gctcggccgg
      241 catgaaagtc tggccgcgcc cgccgggaat cggattgaga atgtcggtct tcggattcac
      301 ccgcgtggtc aggcaccaca tgatgtcgtc catcgagtag atgtcaacgt cttccgagac
      361 cgcgatcgcg agccgcatgc cctgggagca ggacaggatc gccgacagga aattgcgctg
      421 ccagccttcc tcgatgcggt tgcgcttctt cacctggatg atgcagccgc cccagtcggt
      481 catgcagtac gggatgtgca cgtcctggac gatgccgggt tgcagccggt tgcacagctc
      541 gaagatggcc gcctcgcgca ccgtggtgtc gatgttgttg tcgtccatcg tgtgcacgcc
      601 gagcgggaag atgatgggcc ggctctcggg cttgcgcatt gtgatggcgg tgacgtgaaa
      661 tgtcggcgcc ttgtacgcct tgcccatgta gccggcccat tcgggatgga aaaacgtctt
      721 gccctgcacg ccggcctttt cggcttcgct cgtttcgtag cgcttgtcgc gcggatggat
      781 gtggccctcg agcacgtatt cggaatcggc cagcgcgtag gcgtccaccg tgcgcgcctt
      841 caccagtcgc accggcgacc cctgcaccgc gccggcgacg ccgatctcgt cacagccctt
      901 gggcaggatg acgtagtcga acccggcgcc ggcgagcagc gtgcacgccg gcggcacgcc
      961 gaagcacatg gtgagcggaa tcggcctgtc gtcgtcgtaa tgcgcggtat agacctgcca
     1021 catgtgcgag cccggagaaa tctggaaggt gccgatgttg ccccagcgaa agttcatgcg
     1081 gttgtagccg aggtcgctgc cgccgtcgaa atacgcgccg gtgacgcagc ggatgccgga
     1141 acccacggtc aattcgggct cgagcgccgt gtgccggatc gccgtgatcc atttgttgac
     1201 gtcgaggtcg tcgaggatta cgcgctcctg gcacggcgcg tccttctgtt cgatcaccac
     1261 cggcttcagc gggcggttga gggcgtgcgc gatccggcgc gtgcggtccc gcggcccgtc
     1321 gaagccgaac atgtggtcga cgacccgcat gtcgctgaac agattggtga cggcgcgcgc
     1381 atggggcttg cccctgacct tgttgaacag gatcggaaag ctgccgtcga aatgtttctg
     1441 ccaccgctca gtatgaaacg ccactgatag tgcccgtacg ataccccctg gtattggccg
     1501 ctggacagga ggttgtcgag caagcgtatc gtgaggtcgg ccgggccgac gcttaccaaa
     1561 aagacaccgt acggtatgtc gccggcgacc aaatggcgtt tgcttcaacg gtcaacggca
     1621 tgctcatgcg cgaacgaccg gccgccaatg tcttcatggg cgccttcttc gccgagtc
//
LOCUS       DBNGWZ010000127         4428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1397711, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000127 DBNGWZ010000000
VERSION     DBNGWZ010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1397711"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="AC16CX_03820"
     CDS             complement(<1..1520)
                     /locus_tag="AC16CX_03820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3011673.1"
                     /translation="MIKAKGERRKAEGSIHDPQSTTHAVSGGLNEGRRRFMVGAAGLT
                     FGVAFGVPELLRLSSGEAAAAAAKSVVVNPWVTLYTDGTVAIMSPAAEMGQGSLTSLP
                     VIVADEMDADWAKVRIVPAPPRGKLYGNPLFGGEQYTAGSATVTGYFNNLRQFAAQVR
                     YVLMDNAARHWGVPMSELATEPGFVVHAKSGRRISYGEIAAFAQVPAQAPEVEVEPVS
                     KAQFRLIGKDVPRVDVPGKANGTAMYSIDVQVPGMVYGAILREPVEGAAPERIDDAAA
                     RAVEGVIQIVPLKYGVGVLAETPWAAFKAKDALKVTWSRKATGWGFSNEKGFEQFSAI
                     GKDVSRKGFAWDSKGDMAAGMAKAATIVEGEYRADYCYHAQMEPLNSVAAVSPAGDAV
                     EVWVGTQSQTITVVAVAKALGIPESRVKFNGLLLGGGFGRRGNRDVEFIVDSVLMSKA
                     AGRPVKVIWTREDDVHNGRFRPLYVNRIRAGLDASGKIVAWHHRVVCDEVLAFMDPV"
     gene            complement(1523..1990)
                     /locus_tag="AC16CX_03825"
     CDS             complement(1523..1990)
                     /locus_tag="AC16CX_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3011674.1"
                     /translation="MSQMVTTTLTVNGKRQSVTAHPDTPLLWVLREQLKLTGTKFGCG
                     VGQCGACTVHIDGKPNYACLNKIADLAGRSVTTIEGLAPDSSHPLQKAWIAEQVPQCG
                     YCQSGQIMRAAGLLKEHPQPTREQIVDYMSANICRCGTYYRIVRAIERAAKEA"
     gene            2537..3766
                     /locus_tag="AC16CX_03830"
     CDS             2537..3766
                     /locus_tag="AC16CX_03830"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR3011675.1"
                     /translation="MIPGTNRAKFAASRIAFAAVGATCAMLPWVSPADESVTAKPAFT
                     DSEIRIILSHGPWPPPVPHDPTNRVSGKRDAIEFGMRLFFDQRLSGKGTVSCATCHIP
                     ERNWTDNLRRSAGMAEMDRNTPTLMNLLGARWYGWDGAADSLWFQSLRPILDERELAA
                     TPRHVARLVRNDDQLSCRYRKAFGAPPSLTDDDAVLVDVGKALAAFQETLMSGSTPFD
                     QFRDALARGAPPSSWNYSEPAQRGLKIFIGKGVCTSCHAGPNFTSGEFFSTGLSRPVQ
                     PGKSDPGRRAGIRQLLESRFNLLGPYNDDAMGASAARTRQASLEKANFGEFKVPSLRN
                     LMLTSPYGRDGRVDTLAEVVGHYAGLDPVSLHAKDGQPARPLNLTPREQSDLVVFLES
                     LSTFSNPWRLEDDGQCH"
     gene            complement(3796..>4428)
                     /locus_tag="AC16CX_03835"
     CDS             complement(3796..>4428)
                     /locus_tag="AC16CX_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR3011676.1"
                     /translation="ARDERKAAHLRRLYEQYAEQFRVAGNQEAPSVARRVHGAMDAVL
                     ERDRKKSASSGDIRCRKGCDHCCHGPVEIWPHEAALLVGVARGAGMEMDKARLERQSR
                     YTMDNWRQQPAADRACVFLGDDGACKVYEFRPNACRKLLVLSDPALCDADKHPPDLVD
                     RWFSWEAEVLESAALETFGAALMPVSLLAVLDGRGHESPANGADGATMPP"
BASE COUNT          715 a         1569 c         1363 g          781 t
ORIGIN      
        1 acggggtcca tgaacgcgag cacctcgtcg caaaccacgc ggtgatgcca cgcgacgatc
       61 ttccccgatg cgtcgaggcc ggcgcggatg cggttcacgt agagcgggcg gaagcgcccg
      121 ttgtgcacgt cgtcctcgcg cgtccagatc accttcaccg gacggcccgc ggccttcgac
      181 atcagcacgg agtccacgat gaactcgacg tcgcggttgc cgcgccgccc gaatccgccg
      241 cctagcagca ggccgttgaa cttcacgcgg ctctccggga tgccgagcgc cttggcgact
      301 gcgacgacag tgatcgtctg gctctgcgtg ccgacccaga cctccaccgc atcgcccgcc
      361 ggggataccg ccgcgacgga gttgagcggc tccatctgcg cgtggtagca atagtcggcg
      421 cgatactcgc cttcgacgat ggttgccgct ttcgccatcc cggccgccat atctcccttg
      481 ctgtcccacg cgaaaccctt gcggctcacg tccttgccga tcgccgagaa ctgctcgaaa
      541 cccttctcgt tggaaaagcc ccatcctgtc gccttgcggc tccacgtcac cttgagcgcg
      601 tccttcgcct tgaacgcggc ccacggcgtc tcggcgagca cgcccacgcc gtacttgagc
      661 ggcacgatct ggatcacacc ctcgaccgcg cgcgccgcgg cgtcgtcgat tctctccggc
      721 gccgcgccct ccaccggctc gcgcaggatc gccccgtaga ccatgccggg cacctgcacg
      781 tcgatgctgt acatggccgt gccgttcgcc ttgcccggca cgtccacccg cggcacgtcc
      841 ttgccgatca gccggaactg cgccttggag acgggctcga cctccacttc aggcgcctgc
      901 gccgggactt gcgcgaaagc cgcgatctct ccgtatgaaa tccggcggcc ggatttcgca
      961 tgcacgacga agcccggctc ggtcgcgagc tcggacatcg gcacgcccca gtggcgcgcg
     1021 gcattgtcca tcagcacgta acgcacctgg gcggcaaact ggcgcaggtt gttgaagtag
     1081 cccgtgaccg tggcgctgcc cgcggtgtac tgctcgcctc cgaacagcgg attgccatag
     1141 agcttgcccc tgggaggagc cgggacgatc ctgaccttcg cccagtcggc gtccatctcg
     1201 tcggccacga tcaccggcag cgaggtgagc gagccctggc ccatctccgc cgccggcgac
     1261 atgatggcga cggtgccgtc ggtgtacagc gtcacccacg gattgaccac gacgctcttc
     1321 gccgcagccg cggccgcttc gccgctcgac agcctgagaa gctctggaac gccgaaagcg
     1381 acgccgaagg taagtcccgc tgcgccgacc atgaagcgac ggcgcccctc gttcagccct
     1441 ccggaaacgg cgtgagtcgt ggattgtgga tcgtggatcg acccttccgc ctttcgcctt
     1501 tcgcctttcg ccttgatcat ggctatgcct ccttcgccgc gcgctcgatg gcgcgcacga
     1561 tcctgtaata ggtcccgcag cggcagatgt tggcgctcat gtagtccacg atctgctcgc
     1621 gcgtcggctg cggatgctcc ttgagcagtc ccgccgcccg catgatctgt ccggactggc
     1681 agtagccgca ctgcggcacc tgctccgcga tccacgcttt ctgcaacgga tggctcgaat
     1741 cgggcgccaa gccctcgatc gtcgtgacgc tgcgcccggc gaggtccgcg atcttgttga
     1801 ggcaggcgta gttcggcttg ccgtcgatgt gcacggtgca cgcgccgcac tggccgacac
     1861 cgcagccgaa cttggtgccg gtgagcttca gttgctcgcg cagcacccac aatagcggcg
     1921 tgtcgggatg cgcggttacg ctttgacgct tgccgtttac ggtcagcgtc gtggtgacca
     1981 tctgggacat ggtgtctcct ccatagtacc aatcgcgcga atggctcgaa ccattcgcgc
     2041 cctcgaacct cccctcgccc tcagggagag gggcgtgagg gtggtgcgcg ccgtcttcgt
     2101 tccccggaag cgtgtgccgt ttgttataag gcacgcgcgt taattctagt gccgttccgg
     2161 cttaatggga atgccaatcg aaagggttcg cgcgaaattt tcttcgctgc agcgcagcgc
     2221 ggccggatcc attgtagcgc cgattcgccg tcgaaaagct tcgccgccaa gggcgcaaag
     2281 gaacagaaga gctgaaccgc caagcacgcc aaggccgcca aggaaaggaa cagcctcacc
     2341 gcgaaggccg ccaagcccgc gaaggaatca ataataagaa caagggcgcg aagacaacgc
     2401 taagagtggc atacaggatg cgcactggtc gttcttaccc ctttcgcctt catccttcgt
     2461 cgccacgcgc ccgctgtggc gccgggcggg gattggcgcg atacttcgac aaagtccagt
     2521 ccgaccctct cttccaatga tcccaggtac caaccgcgcg aaatttgctg cgtctcgcat
     2581 cgctttcgcc gccgtcggcg caacctgcgc gatgctgccg tgggtgagcc cggcggacga
     2641 gagcgtcacg gcaaagcccg ctttcaccga cagcgagatc cggatcatcc tgtcccacgg
     2701 gccgtggccg ccgcccgtgc cgcatgaccc gaccaaccgg gtttcgggca aacgggatgc
     2761 gatcgagttc gggatgcgcc tgttcttcga tcagcgcctc tccggcaagg gcacggtgtc
     2821 ctgtgcgacc tgccacatcc cggagcgcaa ttggaccgac aacctccggc gcagcgccgg
     2881 catggccgag atggaccgca acacgccgac gctgatgaat ctgctgggcg cgcgctggta
     2941 cggctgggac ggcgccgccg actccctgtg gttccagagc ctgcgcccga tcctggacga
     3001 gcgcgaactg gcggcgacgc cgcgtcatgt ggctcgattg gtgcgcaacg acgaccagct
     3061 ctcctgccgc taccggaagg cgttcggagc cccgccatca ctcaccgatg acgatgcggt
     3121 gctcgtggac gttggaaagg ccctcgccgc cttccaggaa accctgatga gcggaagcac
     3181 gccgttcgac cagttccgcg atgccctggc gcggggggca ccgccttcct cctggaacta
     3241 ttccgagccg gcgcagcgcg ggctcaagat cttcatcggc aagggcgtct gcacgtcctg
     3301 ccatgccggc cccaatttca ccagcggcga gtttttcagc accggcctgt cgcgcccggt
     3361 gcagcccggc aagtccgatc ccggacgccg tgcgggcatc cggcaactgc tggaaagccg
     3421 cttcaatctg ctaggtccct acaacgacga cgcgatgggg gccagcgctg cgcgcacgcg
     3481 tcaggcatcc cttgagaagg cgaatttcgg cgagttcaag gtgccgtcgc tgcgcaacct
     3541 gatgctgacc tcgccctacg gccgcgacgg ccgcgtggat acgctcgccg aggtcgtggg
     3601 gcattacgcc ggtctcgatc ccgtcagtct gcacgcgaag gacgggcaac ccgccaggcc
     3661 cctcaacctc accccgcgcg agcagagcga cctcgtcgtc tttctcgaat cgctgagcac
     3721 cttctccaat ccctggcgcc ttgaagacga tgggcagtgc cattgaatcg ccagcggccg
     3781 gccggaacgg cgtgattatg gaggcattgt agcgccgtct gctccattcg cgggtgattc
     3841 atgccctctc ccgtccagca cggccaacaa cgacaccggc atcaaggcgg cgccgaaggt
     3901 ttcgagggca gcggactcca ggacctcggc ctcccagctg aaccagcggt cgacgaggtc
     3961 cggcgggtgc ttgtccgcgt cgcatagcgc ggggtcgctc agcaccagta gcttgcggca
     4021 ggcgttgggc cggaattcgt acaccttgca cgcgccgtcg tcgcccagga acacgcaggc
     4081 cctgtcggcc gccggctgct gccgccagtt gtccatggtg taccggctct ggcgttcgag
     4141 gcgcgccttg tccatctcca tgcccgcccc gcgcgcgacc ccaaccagca gcgcagcctc
     4201 gtgcggccag atctcgaccg gcccgtggca gcaatggtcg caccccttgc ggcaccggat
     4261 gtcgccgctg cttgcgcttt tcttccggtc ccgttccagc acggcatcca tcgcgccgtg
     4321 caccctgcgc gcgacggatg gcgcctcttg attcccggcc acgcggaact gttccgcata
     4381 ctgttcatac agcctgcgca ggtgcgccgc ttttctctcg tcgcgcgc
//
LOCUS       DBNGWZ010000128         9666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1271484, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000128 DBNGWZ010000000
VERSION     DBNGWZ010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9666
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1271484"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="AC16CX_03840"
     CDS             complement(<1..920)
                     /locus_tag="AC16CX_03840"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3011677.1"
                     /translation="MRYARSEAKAYGRAHMRGIWAAIPYPFNEKGEIDEAGLRRDIRR
                     YIDELKIEGFFCGGLVGEYWALTLEDRRRGQSIVVEEVADRVPTMPHTGCLNLRDTIA
                     LTQHAQKIGATFAVVGNPPVSTHDPDELYHFFRGLCAEVDIAVSLFNTPICGYSLSPQ
                     LIARIAELDNIFAIKNPLPVEHTDEVRRLAGDKIVVCDPSESRWLDNIVKHRDQVFMS
                     SPDPYLLQVPGRLLMREYTHKAMAGDVAGARKVSGELDAVRRISEKWMNARWREPSVP
                     INVIKYWSELLGFTGRNPREPVRPLTAEQKR"
     gene            1158..2156
                     /locus_tag="AC16CX_03845"
     CDS             1158..2156
                     /locus_tag="AC16CX_03845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011678.1"
                     /translation="MQQPGLHRVIRRAGAVFAAAAAALPAAVDAQVAGYPARPVRMVV
                     PFTAGSPIEIPGRAVGQRLAETMGQQFLIDNRPGASGTIGTELVARAPRDGYTILVTN
                     CSHSSNPSNYKKLPYDSLADFAPITQINVTYGNLLVVHPSVPARSVKEFIALARAAPG
                     KLNYASAGIGSPPHTTGAVFASMAGINLTHVPYKGTSVAFNDVLAGYVEVMFASPTFS
                     HPFIQAGRVRALGIGGPRRAPLFPDVPTFHEAGLTGFDVTCYHGMWFPAGVPAEIVRR
                     VYTEAAKAIALPEVRKHLMDNGLRPVGSSPEEFADFLRKDIAHQASIAKSIGIKPQ"
     gene            complement(2176..3411)
                     /locus_tag="AC16CX_03850"
     CDS             complement(2176..3411)
                     /locus_tag="AC16CX_03850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011679.1"
                     /translation="MFDLRLTREQLEIRETVRDFARREIKPIALERDRLEDFDRRFPW
                     EALDKASRMGLRTMALSEELGGTGADNLTSCIVIEELAVGDAGVAATLGQTSWLAHVW
                     FDRVMTPQQRTRFLPQFLADDRFHLAFAGHEPDTDLGWSYHRPKVPQAGYKTTAVRDG
                     KGDWIINGVKNFITNAPIAKLIAVNVRTKTGKSDAAGTSTILVPHDAPGLTIREHDKV
                     GRRLGSNGEMVFEDCRVPAENLLGEEGKSPFGATEVGRAIPRFQAMNLGIGRAAYEAA
                     LEYARLRVQGSRPIIEHQAVGLALANMAIGLESARILIWQAAWASDHQEAYADGSLPE
                     LPLQTIAKVVTSETMQRVALEAAQIFGGMGVMRELPMQKYVRDALIFLHSESSNDVAR
                     LRIAEALAGYRRTTSTEGE"
     gene            complement(3440..3862)
                     /locus_tag="AC16CX_03855"
     CDS             complement(3440..3862)
                     /locus_tag="AC16CX_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3011680.1"
                     /translation="MKPRITLVTLGVDDLERSLRFYRDGLGLKTEGIVGKEFEHGAVV
                     FFDLQGGLKLALWPRKSLAHDSGLPLGKPSATEFTIGHNVSSRAEVDTVMEQARKAGA
                     IIVKPAHDTFWGGYAGYFQDPDRHLWEVAWNPRWAVPD"
     gene            complement(3938..4297)
                     /locus_tag="AC16CX_03860"
     CDS             complement(3938..4297)
                     /locus_tag="AC16CX_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011681.1"
                     /translation="MKAIGYIALAAAASVSLVFVQALMPTSLGATVFLSAWLLLPYAV
                     LALTLVWAKERPSAAANVVVAVVVGGGGLLFLTDVIFLHPDPQGGIAVFFTPIYQGVG
                     IVMLLPICLWLFGKMNT"
     gene            complement(4628..5557)
                     /locus_tag="AC16CX_03865"
     CDS             complement(4628..5557)
                     /locus_tag="AC16CX_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3011682.1"
                     /translation="MSRPLRLEFAGALYHVTARGNAREDIFRDDGDRATFLDLLGREI
                     AQQHWRLYAYCLMGNHYHLLIETPEPNLTRGMQRLNQVYTQRFNRRHRRVGHVLQGRY
                     KAIVVDKDSYFKELIRYVVLNPVRAKMVRLPERWAWSSYRATAGLSTVPPWLAVDEVR
                     GQFGGGGAVYRRFVAQGIGLPSVWEGLRGQMWLGDENFRDQMQRRLGGKRSTDVPRAQ
                     REPARPNPDELLTEVAKAFDLPRHAVLDRRHAQAYRCAVYLLRRVVNEPIAKVARRAG
                     ISAPRVSQIQAEAESARPQGPMRMLLNYYKVKR"
     gene            6017..7159
                     /locus_tag="AC16CX_03870"
     CDS             6017..7159
                     /locus_tag="AC16CX_03870"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3011683.1"
                     /translation="MPTNEPRSSRADRALARARVRFPVHGKSWLLGAAALLLAAWGSH
                     WVYYRWTHVYLDDARIDGEVVSISSRVSGWITELPVIEGDSVKKGQLLARIDARDMVL
                     QREVLVARLKALENQSAVTQAQIGQVDQETLGKYESETSRLAAAQAEVASLAAQARQA
                     LDDYQRAKELAEAKWLSPQAMERARTTYEQVRENHRKALSEVAAARGTLSSAGGSRRQ
                     LQVIARQLIVLGHQADEIRAEIRRRDADIADRTISAPTDGRVVMTFARQGEHVSPGQR
                     ILMFHDPDEIWVEANVRETEVRMLKPGMKAEVRVDAYPGKVFAAEVFRIGQTATSKFA
                     LLPDPNPSGNFTKITQRLPVRIRLADKDPALRPGMMVEVSIAVRND"
     gene            7140..8690
                     /locus_tag="AC16CX_03875"
     CDS             7140..8690
                     /locus_tag="AC16CX_03875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR3011684.1"
                     /translation="MPSETTETLFGRYGPGYRWLATVTVMLGTVAAVLTTTSVNVAIP
                     DIMGAFGIGQDRAQWLSTGTLAAMTVGMLLNAWMMTSFGQRRTFIGALGIFVASLVLA
                     GASPNESVLIFCRIVQGAVAGVLQPLSMYTLFRVFPPEQRGTAMGFFGMSVILGPALG
                     PTLGGVMIDQFNWRYIFYVAVPVSAVAILMGSLFLPEREEEGARVRFDWPGFLLLATA
                     LACLLTGLSNGQREGWHSDIILALFAVAILAAVGFVAWELRAPQPLVNLKVLANSQFT
                     GAAVVAWLFGVGLFGSTYLVPLFVQTVQHLTPYAAGLMLMPAGLIMGALMPMAGYLSD
                     RFPARTLIIAGLLCFAVSSWWLAGVDANMSFWTVAWCVIVSRLGLSLIKPALNVAALR
                     SLRPELLGQGAGMINFSRQLGGAFGVNVLSVILDRRTFFYSDTLTSLQTAANGATADL
                     LRQVEALLAQAGASSDLQSAGALHFLGRVVYAQAYTMGFRDSFLVVAIVFALGLIPAW
                     IMGRAGRR"
     gene            8789..>9666
                     /locus_tag="AC16CX_03880"
     CDS             8789..>9666
                     /locus_tag="AC16CX_03880"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3011685.1"
                     /translation="MDTIDSGSKMAFELRMLGPGFAAEVRGVGLADVAERDDAYLAVR
                     AAFEEYSVLLFRGQTITNELQLAYSKRFGPLEIAKAASRGEGTPFSVLTNVEPDGSVV
                     PPDHKEALRARANQLWHTDSCFKDPPALASVLSARVIAPVGGETEFASQRLGWERLPE
                     PMRARLKDAYAWHDYAHSRGKINPGMISQRERTTLPPVCWRIHWRNPVNGRDSLYIAS
                     HTYAIDGMPQGEAQALLAELIGHTTAPGHTYLHRWQPGDVIMWDNRCVLHRGRPWPDD
                     KPRHVARTTITATDADG"
BASE COUNT         1703 a         3191 c         2994 g         1778 t
ORIGIN      
        1 cgcttctgct cggcggtgag cggcctgacc ggctcgcgcg ggtttcggcc ggtaaagccg
       61 agcagctcgc tccagtactt gatgacgttg atcgggacgc tgggctcccg ccacctggcg
      121 ttcatccact tttccgaaat ccgtcgcacc gcatccaact cgccggacac tttgcgcgcg
      181 cctgcaacat cgccggccat cgccttgtgc gtgtactcgc gcatcagtag ccgtcccgga
      241 acctgcaaga ggtagggatc ggggctcgac atgaacacct ggtccctgtg cttcacgatg
      301 ttgtcgagcc agcgcgactc gctgggatcg cacacgacga tcttgtcgcc cgccagacgc
      361 cgcacttcat cggtgtgctc caccggcagc gggttcttga tggcgaagat gttgtccagc
      421 tccgcgatgc gcgctatcag ttgcggggaa agcgagtagc cgcagatcgg cgtgttgaaa
      481 agcgataccg cgatatccac ttcggcgcac aggccgcgga agaaatgata cagctcgtcg
      541 ggatcgtggg tgctgaccgg cgggttgccc accacggcga aggtggcgcc gatcttctgc
      601 gcgtgctgcg tgagcgctat ggtgtcgcgc aggttcaggc agccggtgtg cggcatggtc
      661 gggacccggt cggcgacttc ctcgacgacg atactctggc cgcgccggcg gtcttccagg
      721 gtgagcgccc agtactcgcc caccagcccg ccgcagaaga aaccttcgat cttcagctcg
      781 tcgatgtagc ggcggatgtc cctgcgcagc cccgcctcgt cgatttcccc tttttcgttg
      841 aacggatatg gaatggcggc ccagatgccc cgcatgtgtg ccctgccgta cgcctttgcc
      901 tcggatcgag cgtaacgcat gactttcctc cttcggtaaa cgcaacgcca caacgctttg
      961 cgaaagggtg acctgtccgg attagatcgc caagaatacc aggctgtcct tgatgcaggt
     1021 caaaacgagc gctctcccgc ccatcggcgg atgttgactc gcatgctggc ggcctcctaa
     1081 catggcccga aaatactaat cggactccga gcaggtccga gccccggccg cgtaaggcag
     1141 tcccaggaaa cccgagcatg cagcaacccg gccttcatcg cgtgatccgc cgcgccggcg
     1201 cggtcttcgc cgcggccgcc gccgcgctcc ccgccgcggt cgatgcgcag gtcgcaggct
     1261 atcccgccag gccggtgcgc atggtcgtgc cgtttacggc gggcagtccg atcgagattc
     1321 ccgggcgcgc agtcgggcag cggctcgccg agacgatggg ccagcagttc ctgatcgata
     1381 accgtcccgg cgcgagcgga acgatcggca cggagctggt cgccagggcg ccgcgcgacg
     1441 gatacaccat cctggtcacc aactgttcgc actcgtcgaa cccgtccaat tacaagaagc
     1501 tgccgtacga ctccctcgcc gacttcgccc cgatcactca gatcaacgtg acctacggga
     1561 acctgctggt cgtgcatccg tcggttccgg cgcgctcggt gaaggagttc atcgcgctcg
     1621 ccagggctgc gcccggcaag ctcaattacg cctcggccgg catcggcagc ccgccgcata
     1681 ccaccggcgc ggtgttcgcc tcaatggccg gcatcaacct gacgcacgtg ccctacaagg
     1741 gaacctcggt cgcgttcaac gacgtgctcg cagggtacgt cgaggtgatg ttcgcgagcc
     1801 cgacgttctc ccacccgttc atccaggcgg gccgcgtgcg cgcgctcggc atcggcggcc
     1861 cgcgccgcgc gccgttgttt cccgacgtcc cgacgttcca cgaagcggga ctcacgggtt
     1921 tcgacgtcac gtgctatcac ggcatgtggt tcccggccgg cgtgccggcc gaaatcgtgc
     1981 gccgcgtata caccgaagcg gcgaaggcga tcgctctgcc ggaggtgcgc aaacacctga
     2041 tggacaacgg ccttcgtccg gtcggcagct cgccggaaga gttcgccgat tttctcagga
     2101 aggacatcgc gcaccaggcg agcatcgcga agagtatcgg catcaagccg cagtgatgga
     2161 gaatttccgg aacggtcact ctccttcggt agaagtcgtt ctccgatagc ccgcaagggc
     2221 ttctgcaatc cgcaggcgtg cgacgtcgtt gctgctttcg gaatgcagga agatcagcgc
     2281 gtcgcgcacg tacttctgca tcggcaattc gcgcatgaca cccatgccgc cgaagatctg
     2341 cgccgcctcg agggcaacgc gctgcatcgt ctcggaggtg acgaccttgg caatggtctg
     2401 gagcggaagc tcgggcagac tgccgtcggc ataggcttcc tgatggtccg aggcccaggc
     2461 cgcctgccag atcaggatgc gcgccgattc gagaccgatc gccatgttgg cgagcgcgag
     2521 ccccacggcc tgatgttcga tgatcggccg gctaccctgg acccgcagcc gggcatactc
     2581 gagcgcagcc tcgtaggcgg cgcggccgat gccgagattc atcgcctgga agcgcgggat
     2641 ggcgcgcccc acctccgtcg ccccgaatgg gctcttgccc tcctcgccga gcaggttttc
     2701 cgccggcacg cggcaatcct cgaataccat ctctccgttg gaacccagcc tgcgcccgac
     2761 cttgtcgtgc tcgcgtatcg tgagccccgg cgcgtcgtgc ggaaccagta tcgtgctcgt
     2821 accggctgcg tcggacttgc cggttttggt cctgacgttc acggcaatga gttttgcaat
     2881 cggcgcgttg gtgatgaaat tcttgacgcc attgatgatc cagtcaccct tgccgtcgcg
     2941 caccgcggtg gtcttgtagc ccgcctgcgg aactttcggg cggtggtaac tccaaccgag
     3001 atcggtatcg ggctcatgcc cggcaaacgc gagatggaat cggtcgtcgg ccagaaactg
     3061 cggcagaaag cgcgtgcgct gctgcggcgt catcacccgg tcgaaccaga cgtgcgcgag
     3121 ccacgaggtc tgtcccaggg tcgcggcgac ccccgcgtcg cccaccgcaa gctcttcgat
     3181 gacgatgcag gaggtcaggt tatcggcgcc ggtgccgccg agttcctccg acaaggccat
     3241 cgtgcgcaat cccatccgcg aggccttgtc gagcgcttcc cacgggaaac gtcgatcgaa
     3301 atcttccagg cggtcgcgct ccagcgcgat cggtttgatt tcccgccgcg cgaagtcgcg
     3361 cacggtctcc cggatttcaa gctgctcccg cgtaagtcga agatcgaaca tgattggtcc
     3421 ttctgcaagc tgtagtgcct cagtccggaa cagcccaccg aggattccac gcgacctccc
     3481 acagatgccg gtcggggtcc tggaagtagc ctgcgtaacc tccccagaac gtatcgtgcg
     3541 ccggctttac gatgatcgcg ccggcttttc ttgcttgttc catgaccgta tcgacttccg
     3601 ccctggaaga cacgttgtgc ccgatcgtga attcagtcgc gctgggtttt cccaggggaa
     3661 gacccgagtc atgggcaagg ctcttgcgcg gccagagcgc gagcttcaaa ccaccctgga
     3721 ggtcgaagaa tacgaccgca ccgtgctcga actccttgcc gacgatgcct tccgtcttga
     3781 ggcccaaccc atcgcgatag aaccggagcg atcgctccag gtcgtcaacg ccgagggtga
     3841 caagcgtgat ccggggcttc atgcgtgggt tgcctgttag gcgtaacgta gagctaccgg
     3901 cgcggcgttg tttgccgcat ccaggttgag cggcgagtca ggtgttcatc tttccaaata
     3961 accataggca gatcggcagc aacataacta tcccgacacc ttgataaatg ggtgtaaaga
     4021 aaactgcaat tccaccttgc ggatcgggat gcaggaaaat gacatcggtc aggaatagca
     4081 atccgcctcc ccctaccacc accgcgacca caacatttgc agccgccgat ggcctctcct
     4141 ttgcccagac caaggtcaac gcaaggactg catagggaag taacagccat gcgctaagga
     4201 atacggttgc tccaaggctg gttggcatta gggcctgtac gaacacgaga cttaccgaag
     4261 ctgctgcggc gagagctatg tagccgatag ccttcatgac tttttggtca tctctctcgc
     4321 acttaacgtc ccggctcagg cgcgcgccgc ttctggcgcg tcgccctgca gccggcggtt
     4381 agcctgcatt gcgtcatcac gaggttcgtc atctaccgtc gtgacgctgc cttcccaggc
     4441 gttcagcaag agcccagcac agcgtgtcag cagcaccgct cgtcagtcgg gcaatctcga
     4501 tcgattaaaa caaacaacaa aaacaacaaa caacaaaggg gtcaaaagtc aaaagaatgt
     4561 caaaagcaaa aggggtcaat caaaagcaaa aggggtcaac actttacctt atcaaaagca
     4621 aaaggggtca acgctttacc ttatagtaat tcaacagcat ccgcatcggc ccttgcggcc
     4681 tggcgctttc cgcctcggcc tgaatttgcg acacccgtgg ggcggatata ccggcacgcc
     4741 gcgcaacctt ggcgatcggt tcgttcacca cccgccgcag cagataaacc gcacaacgat
     4801 acgcttgcgc gtggcggcgg tcgagcaccg catggcgcgg aagatcgaat gccttcgcaa
     4861 cttctgtaag cagttcgtcc gggttcgggc gagcgggctc gcgctgcgca cggggaacat
     4921 ccgtactgcg cttgcctccg agacgacgct gcatctggtc ccgaaagttt tcatcgccga
     4981 gccacatctg cccgcgcaaa ccctcccaca ccgagggcag gccgatgccc tgcgcaacga
     5041 atcggcggta gactgcgccg cctccgccaa actgcccgcg cacctcatcc acggcaagcc
     5101 acggcggcac cgtgcttaat cctgcagtcg cacggtagct cgaccacgcc cagcgctcgg
     5161 gtagtctcac catcttcgct cgcaccgggt tgagcacgac gtagcggatc aactccttga
     5221 agtagctatc cttgtctacc acgatcgcct tgtagcgacc ttggagcacg tggcccaccc
     5281 gccggtggcg tcggttgaag cgctgggtgt agacctgatt gagccgttgc atgccgcgtg
     5341 tcaagttggg ctcgggggtt tcgataagca ggtggtagtg attgcccatc aggcaatagg
     5401 cgtagagtcg ccagtgctgc tgggcgatct cgcggccgag cagatcgaga aacgttgcgc
     5461 ggtccccatc atcgcgaaag atgtcctcgc gcgcattgcc gcgcgcggtg acgtgataca
     5521 acgcgccggc gaattccaga cgcagcggac gggacatgac aagatgctaa tacgcccacc
     5581 ctataaggta aagcgttgac ccctatcact tttggccgca ctgccatgac tcaagatggc
     5641 cactacgaag aaaatcgccg ctgctcccac aaccgtaaca gtctttgtga acagccgttc
     5701 gtgcgtcgtg caagcaccga tccctgcgca agaacacgtc accatgactt cctcctcgta
     5761 agtgctccag ccgaacgtat agtgaacttc ctaattgtcg tctaatttgg cggctggctg
     5821 cgcacgtttc tgattttgaa aaataagggc cgggcttcga attatcataa ttcgaagcct
     5881 ggcccgtttt attgctttta ttcgttgagg gccacatatt ggaatcctcc tcgcttgctg
     5941 cattgcgagt gtcctaaact ggtcatggtt tccggccggg ctccccgccc agctccatgc
     6001 ggagtagcgt cgttccatgc caacgaacga gcctcgttcc tctcgcgccg atcgcgcgct
     6061 ggcgcgcgcc cgcgtgcgtt tccccgtaca cgggaagagc tggctgctcg gcgcggccgc
     6121 cctgttgctc gccgcatggg gctcgcactg ggtgtactac cgctggactc acgtctacct
     6181 ggacgacgca cgcattgacg gcgaggtcgt ctcgatctcg agccgcgttt cgggatggat
     6241 cacggaactc cccgtgatcg agggcgacag cgtgaagaaa ggacagctgc tcgcccggat
     6301 cgatgcccgc gacatggtgc tgcagcgcga agtcctcgtc gccaggctca aggcgctcga
     6361 aaaccagtcc gcggtgacgc aggcgcagat cggccaggtc gaccaggaaa cgctcggcaa
     6421 gtacgaaagc gagaccagcc gcctcgccgc ggcgcaggcg gaggtcgcgt cgctcgccgc
     6481 gcaggccagg caggcgctcg acgactatca gcgggcgaag gagctggccg aggcaaagtg
     6541 gctttcgccg caggcgatgg agcgcgcgcg caccacctac gagcaggtgc gcgagaacca
     6601 ccgcaaggcg ctatccgagg tggcggccgc gcgcggcacg ctctcctccg ccggcggcag
     6661 ccgccgccag ctccaggtga tcgcacggca gctgatcgtg ctcggacacc aggcggacga
     6721 aatccgcgcc gaaatccgcc ggcgcgacgc cgacatcgcc gaccgcacta tctcggcccc
     6781 gaccgacggc cgcgtcgtca tgaccttcgc gcgccagggc gagcacgtgt ctcccggaca
     6841 gcgcatcctc atgttccacg acccggacga gatctgggtc gaggcgaatg tgcgggagac
     6901 cgaagtgcgg atgctcaagc cgggcatgaa ggcggaggtc cgcgtcgacg cctaccccgg
     6961 gaaggttttt gccgcggagg tcttccggat cggacagacg gcgaccagca agttcgcgct
     7021 cctgcccgat cccaatccga gcggcaactt caccaagatc acgcagcgcc tgccggtgcg
     7081 catccgtctc gccgacaagg acccggcgct gcgcccgggg atgatggtgg aggtttccat
     7141 tgccgtccga aacgactgaa acactgttcg ggcgctacgg cccgggctac cgctggctcg
     7201 ccactgtcac cgtgatgctg ggcacggtgg ccgcggtgct gacgacgacg tcggtcaacg
     7261 tcgcaatccc ggacatcatg ggcgccttcg gcatcgggca ggaccgggcg cagtggcttt
     7321 ccaccgggac gctggcggcg atgaccgtcg gcatgctgct caacgcctgg atgatgacga
     7381 gtttcgggca gcgcaggacc ttcatcgggg cgctgggcat cttcgtcgct tcgctggtgc
     7441 tggccggcgc gagtcccaac gaatcggtgc tcattttctg ccggatcgtg cagggtgccg
     7501 tggcgggcgt gctgcagccg ctgtccatgt acacgctgtt ccgggtattc ccgcccgagc
     7561 agcgggggac cgccatgggg ttcttcggca tgagcgtgat cctcggcccg gcgctgggcc
     7621 cgacgctggg cggggtgatg atcgaccagt tcaactggcg ctacatcttc tacgtcgcgg
     7681 tcccggtgag cgccgtcgcg atcctgatgg gcagcctgtt ccttccggag cgggaggaag
     7741 aaggcgcgcg ggtgcggttc gactggccgg gattcctgct gctggcgacg gcgctggcct
     7801 gcctgctgac ggggctgtcg aacggccagc gcgaaggctg gcattccgac atcatcctcg
     7861 ccctgttcgc ggtcgccatt ctcgccgctg tcggctttgt cgcctgggaa ctgcgcgcgc
     7921 cgcagccgct ggtcaacctg aaggtgctgg ccaattcgca attcaccggg gcggccgtcg
     7981 tggcttggct gttcggcgtg gggctgttcg gttccaccta cctggtgccc ctgttcgtgc
     8041 agaccgtgca gcacctgacg ccctacgcgg ccgggctcat gctgatgccg gcgggactga
     8101 tcatgggcgc tctgatgccc atggccggct acctgagcga ccggtttccc gcgcgcaccc
     8161 tgatcatcgc ggggctgctg tgcttcgccg tgtcctcctg gtggcttgcg ggtgtcgatg
     8221 ccaacatgtc gttctggacc gtcgcgtggt gcgttatcgt ctcacgcctc ggtctcagcc
     8281 tgatcaagcc cgcgctcaac gtcgcggccc tgaggtcgct gcggccggag ctcctcgggc
     8341 agggagcggg catgatcaac ttctcgcgcc agctcggcgg ggccttcggc gtgaacgtgc
     8401 tgtcggtcat cctcgaccgc cgcacgtttt tttacagcga caccttgacc agcctgcaga
     8461 ccgcggccaa cggcgcgacc gcggacctgc tgcgccaagt ggaggctttg ctcgcgcagg
     8521 ccggcgcgtc gtcggacctt cagtcagcgg gcgcgctgca tttcctcggc cgcgtcgtct
     8581 acgcccaggc gtacacgatg ggctttcgcg acagcttcct cgtcgtcgcg atcgtcttcg
     8641 cgctcgggct gatccccgcg tggatcatgg gccgggccgg tcggcggtag acaaagaggg
     8701 tcaaagcccg tcacctatca cttatcaccg ggtttagatc agccctccgc cctcatccct
     8761 catccctgga tgggaagtaa aatagcatat ggataccatc gactctggca gcaagatggc
     8821 gtttgaactg agaatgctcg gtccgggttt cgccgctgaa gtgcgggggg tcgggctcgc
     8881 ggacgtcgcc gagcgcgacg acgcctatct ggcggtgcgg gcggctttcg aggaatactc
     8941 ggtattgctc ttccgcggcc agacgatcac caacgaattg cagctcgcct actcgaagcg
     9001 cttcggcccg ctggagatcg ccaaggccgc ttcgcgcggc gaaggcacgc cgttttccgt
     9061 cctcaccaat gtggaacccg acggcagcgt cgtgccgccc gaccacaaag aggcgctacg
     9121 cgcgcgcgcg aaccagctct ggcacaccga tagctgcttc aaggatcccc ccgcgctcgc
     9181 ttccgttctc tccgctcgcg tcatcgcgcc ggtcggcggc gagaccgagt tcgcttcgca
     9241 gcgcctgggg tgggagcggc tcccggagcc gatgcgcgcg cgcctgaagg acgcctacgc
     9301 ctggcacgat tacgcgcact cgcgcggcaa aatcaacccg ggcatgatct cgcagcgcga
     9361 gcgcaccacg ctgccaccgg tgtgctggcg catccactgg cgcaatccgg tgaacgggcg
     9421 cgactcgctg tacatcgcgt cgcacaccta cgcgatcgac ggcatgccgc agggagaagc
     9481 ccaggcattg ctggccgaac tgatcgggca cacgacggcg cccggccaca cctatctcca
     9541 ccgctggcag cccggtgacg tcatcatgtg ggacaaccgc tgcgtcctgc accgcggccg
     9601 gccgtggcct gacgacaagc cgcgtcacgt ggcccgcact acgatcaccg cgaccgatgc
     9661 cgacgg
//
LOCUS       DBNGWZ010000129         4936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2909502, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000129 DBNGWZ010000000
VERSION     DBNGWZ010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2909502"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC16CX_03885"
     CDS             complement(<1..917)
                     /locus_tag="AC16CX_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3011686.1"
                     /translation="MFNKIVVANRGAVAARVLRALNAMGIHSVAVYSEVDAGAPYLEM
                     AGETYAIGAAPARESYLNQDALIEVLRKSRADGLHPGYGFLSENAAFAQKVADAGARF
                     IGPSPRCIEAMGHKTRARELAASHGMPMTRGSGVLPADLDAALAAARSIGFPVLVKPA
                     GGGGGIGMLPAKNETELAAALERSRSMAERGFGNTEVYLERLIERPRHVEFQVLGDAH
                     GSAVHLFERDCSTQRRNQKVIEEAPAPAIPRERAGAVAEQVAAIMRGMGYDNIGTVEM
                     LLGADGAFSFLEMNTRLQVEHGVTEEVTGV"
     gene            complement(1033..2568)
                     /locus_tag="AC16CX_03890"
     CDS             complement(1033..2568)
                     /locus_tag="AC16CX_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3011687.1"
                     /translation="MAYEALLAEFEARRAKALAGGGAEKYAKRTAAGVWNARERIAYL
                     VDKGTFIESGLFGASGVYPEEADETQTDGKLAGYAKINGRDVCIVVNDFTVKGASTSA
                     TNSKKIAHMKRAATERGMPLVVIGESTGARLPDAMGSRGMGMLLGNDITQFRRMRETP
                     MCSAALGPSFGSSTWLTCQADFAVMQKGATVAVSSPRLVSMALGEKVEPEELGGWRLH
                     AEVTGLVDQVVDKEEEVFDAIKRFLSYLPSHHMEAPPDAPVPAGSGADMDKVFSMLPE
                     SRTQVYDMKRIIRALVDKDTLFELKPRFGKSAVVGLARLGGKSVGIVANNPLHRGGAL
                     DADACRKIVDFLVLCDSFNVPLVLLVDTPGFQIGTEAERAGAPGKIMNFMNAMTLVTV
                     PRLSVIIRKSYGRAYVCMGGGRHSDDVAAWPTAEVSFMSPEFATKIVHGVGVGEPGFE
                     EKFAQIQKGSDVWGLAEVYAAQAVLRPEETRDYLIRMLGVYKLRTTKGMGQHLMRTWP
                     TSY"
     gene            complement(2669..4237)
                     /locus_tag="AC16CX_03895"
     CDS             complement(2669..4237)
                     /locus_tag="AC16CX_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3011688.1"
                     /translation="MSWSAEIKEIHRRRGLAEECGGSEAVARHHAEGKLTVRERIERL
                     LDAGSFQEVGKLAGRAQYDANGQLTSFVPHPYVAGIGRIDGRPVGVGGEDYTIKGGSG
                     SGSGRRKGGQGGFIEDLAARYRIPLVNLIDGVGGSVESIERRGYSTFPGAGQDGFERS
                     VELLGVVPVVSAVMGTAAGGPSGRAILSHWTIMVKGTSQIFAAGPPVVERAYGKKLGK
                     EELGGSQMAVDTAGTIDNAAENEEDCLRQIRRFLSYLPTNVWELPPEVQCDDSPDRCE
                     DALADIIPRSPRQPYDMRKLIAMVVDRDSVFEIQPTFGRAVITCLARLAGKVVGVIAY
                     NPMHGGVIDVKAARKQTHFTELCDTFNIPLVFLVDVPGFQIGMESEAAGMLRIGMQGR
                     FLNMQLTVPVYTVVVRKCYGMAGGSTVDRAGLNFKIAWPSAEWGSLPIEGGVKAAFRR
                     EIESAPDPAQREREIELEIRAHASPFRTADAFAVEDLIDPRETRPYLCRFLEAAWPGI
                     RTRLGPKYRTGVRP"
     gene            4395..>4936
                     /locus_tag="AC16CX_03900"
     CDS             4395..>4936
                     /locus_tag="AC16CX_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3011689.1"
                     /translation="MTAAAGLAGQATGASSSAPLTLSLAEQIAARLSERIIAGIYAPG
                     QRVMEQTVSAEFGVSRGPVREALRLLEKDGLVTILPRRGAQVTQLSIAEVREIFDIRA
                     ALNGLRDRQIAEDPDRGRLLPALESTVAQLARLASDPGEGDEYVETVFELNRMLNQAA
                     PNRRLRAILSSLALQTLRYSR"
BASE COUNT          796 a         1719 c         1532 g          889 t
ORIGIN      
        1 acgccggtca cttcctcggt cacgccgtgc tcgacctgca gccgcgtgtt catctcgagg
       61 aagctgaaag cgccgtcggc gcccagcagc atctccaccg tgccgatgtt gtcgtaaccc
      121 atcccgcgca tgatcgcggc cacctgctcc gccaccgcgc cggcccgctc gcgcggaatg
      181 gcgggagccg gtgcttcctc gatcaccttc tggttgcggc gctgcgtcga gcaatcgcgc
      241 tcgaacaggt ggaccgcgct gccgtgcgcg tcccccagca cctggaattc gacgtggcgc
      301 ggccgctcta tcagccgttc gagatagact tccgtgttgc cgaagccgcg ctcggccatc
      361 gagcgcgagc gctcgagcgc ggcggcgagc tccgtttcgt tcttcgccgg cagcatgccg
      421 attccgccgc cgccgcccgc tggcttaacc aatacgggaa agccgatcga gcgcgctgcc
      481 gcgagcgcgg cgtcgaggtc ggcaggcagc acgccagaac ccctggtcat cggcatgccg
      541 tggcttgcgg ccagctcgcg cgcccgggtc ttgtgcccca tcgcctcgat acagcgcggc
      601 gatggcccga tgaagcgcgc gccggcgtca gccaccttct gcgcgaacgc cgcattctcc
      661 gaaagaaagc cgtagccagg gtgcagcccg tcggcgcgtg atttgcgcag cacctcgatc
      721 aacgcgtcct ggttgaggta gctctcgcgc gccggcgccg cgccgatggc ataggtctcg
      781 cccgccattt ccaggtacgg cgcgcccgcg tcgacttccg aatacacagc gaccgaatgg
      841 atgcccatgg cattgagcgc ccgcagcacg cgcgcggcta cggcaccgcg gttggcaaca
      901 acgatcttat tgaacatgac aaggcgtgat aggtgataag tgataggtga taggagttcc
      961 cgcctcggag gccgaggcgg ggaatagcga tcggggtgca cttatcactt atcacccgtc
     1021 acttatcacg ctttagtaag acgtcggcca ggtccgcatc aaatgctgcc ccatgccctt
     1081 cgtcgtgcgc agcttgtaga cgccgagcat gcggatcagg tagtcgcgcg tctcctcggg
     1141 gcggagcacg gcctgcgccg cgtagacttc ggcgagcccc cacacgtcgg accccttctg
     1201 gatctgcgcg aacttctcct cgaagcccgg ctcgcccacg cccacgccgt gcacgatctt
     1261 ggtcgcgaac tcggggctca tgaagctcac ttccgccgtc ggccacgccg cgacatcgtc
     1321 cgagtgccgc ccgccgccca tgcacacgta ggcgcgcccg tagctcttgc ggatgatcac
     1381 cgagaggcgc ggcacggtga ccagcgtcat cgcgttcatg aagttcatga tcttgcccgg
     1441 cgcccccgcg cgctcggcct cggtgccgat ctggaagccc ggcgtgtcca ccagcagcac
     1501 gagcggcacg ttgaaggaat cgcacagcac gaggaaatcg acgatcttgc ggcaggcgtc
     1561 ggcatccagc gctccgccgc ggtgcagcgg attgttggcg acgatgccga cgctttttcc
     1621 gccgagccgc gcgagaccca cgactgcgct cttgccgaag cgcggcttca gctcgaacag
     1681 cgtgtccttg tccaccagcg cgcggataat gcgcttcatg tcgtagacct gggtgcggct
     1741 ctcgggaagc atcgagaaca ccttgtccat gtccgcgccc gatcccgcag gcaccggcgc
     1801 atcgggcggc gcttccatgt ggtggctcgg caggtaggag aggaagcgct tgatcgcgtc
     1861 gaacacctct tcttccttgt ccaccacctg atccaccagt ccggtcacct cggcgtgcag
     1921 ccgccagccg cccagctcct ccggctccac cttttcgccc agggccatcg agacgaggcg
     1981 ggggctcgat accgcgacgg tcgcgccttt ctgcatcacc gcgaaatcgg cctggcaggt
     2041 gagccaggtg gaggagccga acgagggccc cagcgcggcg gagcacatcg gggtttcgcg
     2101 catgcgccgg aattgcgtga tgtcgttgcc gagcagcatt cccatgccgc gcgagcccat
     2161 cgcgtccggc agccgcgcgc cggtcgattc gccgatcacc accagcggca tgccgcgctc
     2221 ggtggcggcg cgcttcatgt gcgcgatctt cttgctgttg gtggcgctgg tggatgcgcc
     2281 cttcaccgtg aagtcgttca ccactatgca gacgtcacgc ccgttgattt tcgcgtagcc
     2341 cgcgagcttg ccgtcggtct gcgtctcatc ggcctcctcg gggtagacgc ccgaggcgcc
     2401 gaacagcccg gattcgatga aggtaccctt gtcgaccaga taggcgattc gctcgcgcgc
     2461 gttccacacc cctgctgccg tgcgttttgc gtacttttcg gcgcccccgc cggcgagcgc
     2521 cttcgcccgc cgcgcctcga attccgccaa taaagcctca tatgccattg ccgtcctctt
     2581 tcgaccgggt tctgctgcca ttttccctat gaaaacgccc ctgttagtaa ccgctcactt
     2641 ttgggcgtgt tttgccccaa atttgacctc aggggcgaac gcccgtccgg tacttcggac
     2701 cgaggcgcgt ccgtatcccc ggccacgccg cttcgagaaa gcggcagaga tagggacgcg
     2761 tctcgcgcgg gtcgatcaga tcctccaccg cgaacgcatc ggcggtgcga aacggcgatg
     2821 catgggcgcg gatttccagc tcgatttcgc gctcgcgctg cgcggggtct ggcgcggact
     2881 cgatttcgcg ccggaatgcc gctttcacgc cgccctcaat cggcagcgat ccccattccg
     2941 ctgacggcca tgcgatcttg aagttgagcc ccgcgcggtc cacggtgctt ccgcccgcca
     3001 taccgtagca cttgcgcacc acgaccgtat agaccggcac cgtgagctgc atgttgagga
     3061 agcgtccctg catgccgatg cgcagcatgc cggccgcttc cgattccatc ccgatctgaa
     3121 agcccggcac gtccaccagg aacaccagcg ggatgttgaa ggtgtcgcac agctcggtga
     3181 agtgcgtctg cttgcgcgcc gccttcacgt cgatcacgcc gccgtgcatc gggttgtagg
     3241 cgatcacgcc cacgaccttg ccggcaagcc gcgccagaca ggtgatcacc gcgcggccaa
     3301 acgtcggctg gatctcgaac accgaatcgc gatccaccac catcgcgatc agcttcctca
     3361 tgtcgtacgg ctggcgcggt gagcgcggaa tgatgtcggc gagcgcgtcc tcgcaccggt
     3421 ccggggaatc gtcgcactgg acctcgggcg gcagttccca aacgttggtc ggcaggtacg
     3481 aaaggaaacg ccggatctgc cgcaggcagt cttcctcgtt ctcggccgcg ttgtcgatcg
     3541 tgccggcggt gtccaccgcc atttgcgagc cgccgagctc ctccttgccg agcttcttgc
     3601 cgtacgcgcg ctccaccacc ggcggtcccg ccgcgaaaat ctggctggtg cccttcacca
     3661 tgatggtcca gtgcgagaga atcgcgcgcc cggacggtcc gcccgccgcg gtgcccatca
     3721 cggcgctcac caccggcacc acgcccagca actcgaccga gcgctcgaag ccgtcctgtc
     3781 cggcgccggg aaacgtgctg tagccgcggc gctcgatgga ctcgacgctg ccgccgacgc
     3841 catcgatcag attcaccagc ggaatgcgat agcgcgccgc gaggtcctcg atgaaaccgc
     3901 cctgcccgcc tttgcgccgc ccgctgcccg agccggagcc gcctttgatg gtgtagtcct
     3961 caccgcccac cccaacgggg cgcccgtcga tcctgccgat gcctgcaacg tacggatggg
     4021 gcacgaaaga tgtgagctgg ccgttcgcgt catactgcgc acggccggcg agcttgccga
     4081 cttcctggaa gctgccggcg tcgagcaggc gctcgatgcg ttcgcgcacg gtgagcttgc
     4141 cttcggcatg gtgtcgcgca accgcttccg acccgccgca ctcctccgca agtccgcgcc
     4201 ggcgatggat ctccttgatt tctgctgacc aactcatggg tgcccccatc tggatagggt
     4261 ctactcttcc gcaaccggtt gatatatatg tagtagatta ggcagtataa cttgtgttgt
     4321 caataagatt gttgacaatc atacaagatt aatctatctt aatcaggtcc ttagccgacc
     4381 ggaaggagca cgacatgact gcagctgcgg ggctggcggg gcaggctacc ggtgcgtctt
     4441 caagtgcgcc gctgacgctt tcgctagccg agcaaatcgc cgcgcgcttg tccgagcgca
     4501 tcatcgccgg catctacgcg cccgggcagc gcgtcatgga gcagacggtg tccgcggagt
     4561 ttggcgtgag ccgcggtccc gtgcgcgaag ccttgcggct gctggagaaa gacggcctcg
     4621 tcacgatcct cccgcggcgg ggggcacagg tgacccagct ttcgatcgcc gaggtgcgtg
     4681 agatcttcga catccgcgcg gcgctgaacg ggttgcgcga ccggcagatc gccgaagatc
     4741 ccgaccgcgg ccgcctgctt ccggctctcg aatcaacggt cgcgcaactg gcgcgactcg
     4801 cgagcgatcc tggggaaggg gacgaatacg tcgagacggt cttcgagctg aacaggatgc
     4861 tcaaccaggc cgcgcccaac cgcaggttgc gcgcgatcct gtcctcgctc gcgctgcaga
     4921 cgctgcgcta ttcgcg
//
LOCUS       DBNGWZ010000130         4108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3286950, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000130 DBNGWZ010000000
VERSION     DBNGWZ010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3286950"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1252)
                     /locus_tag="AC16CX_03905"
     CDS             complement(236..1252)
                     /locus_tag="AC16CX_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011690.1"
                     /translation="MAKVDEFEFFLQKDWSDGFPVVTPTEARVRKMLAGTDRDPDEVV
                     GSIPPAMEVATVRAVAIHALMAGCKPEYLPVVLGGVKLILREEFNMGGVQCTMHGVAP
                     LMIVNGPYAQKIGLHGGNGCFGPGFRANATIGRAIRLILLNLGGGISGRASATVFSSP
                     IRYTACLTENMALSPWESLAASRGYSPEDNVITCAMAENPRLHFDDASTEPQRLLTGI
                     GDAMTAPGSWNMWFVSDMVVAMSPDHARLCARAGMDRKDVVKLLSELAVRRQSELKRG
                     GNWRPERARAMGIDPDDENAPFRAVKDPQRLHLIVAGGTGPITAVCHGWNETSRAVHG
                     AYEV"
     gene            complement(1404..2750)
                     /locus_tag="AC16CX_03910"
     CDS             complement(1404..2750)
                     /locus_tag="AC16CX_03910"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3011691.1"
                     /translation="MQTASHHLAEFAVGLTYDRIPGEVIERAKDCVIDTVAACVFGSQ
                     LPWTQTVIEYAKRNSAPGECSVFGTPIKVRAPFACLTNGASAHAFELDALCEPSVGIH
                     PSAALAVPGLAPAQGRKMSGTTLLSAIVAGYEVLYRIGDAARHSIEKNGFHSPGVVGP
                     FGTAVVVGRMFGLDAKQMANAFGIAGSMSSGLMEFSRTGGMVKRLHLGRAAEGGFMAA
                     VLARDGYTGPGGALDGKFGFLNVFCTDGDATRFTKNLGSEWHVMKTKIKRYACHATAQ
                     VPVTLALGLKAKHGISGDDVARISIAASEKTVTQHAINEPRDLTMMQYSAPFCVAIAL
                     YKDPLDPRVFSEATLGDARIRALCKGAQVELYKKSEGHQSPACRVRLKLKNGKEVSAE
                     GHDFKGTPTMPLTRDELRDKFLKLTAHRDRAKAERLFSRLAEAEKVADFSALDFAL"
     gene            complement(2752..3732)
                     /locus_tag="AC16CX_03915"
     CDS             complement(2752..3732)
                     /locus_tag="AC16CX_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011692.1"
                     /translation="MHRVVTVVAAAFLGAATSAAWCRAPAERYPYRPIRLIQPFPPGG
                     GADVAARFVGQTLTERLGQTVVVDNRGGAGGAIGTELAAQAAPDGYTLLMATASTVVI
                     NPLVNKVNFDPIRDFGPVVHISNVPLILVVHPAVPARTVQDLINLAKAQPGKINFASS
                     GEGTISHLAGELFKLLTGTDMVHVPYRGGGPARTALIGGQVQVNFGNMLATASDVRAG
                     RLRALGVSTAKRTEGMPDVPTMREAGVTQYEVIQWSGMLAPRGVPKVFIERINREVNV
                     ILDQPKAKQFFLAGGSEVAGGAPQAFGALIKSEIAKWSKVIKQSKLEVNR"
     gene            complement(3778..>4108)
                     /locus_tag="AC16CX_03920"
     CDS             complement(3778..>4108)
                     /locus_tag="AC16CX_03920"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011693.1"
                     /translation="FYRLHDPAGAMDAAARDMQERVKFVGREDWTDLQAGHRAIVTVK
                     TRAGQTLTEEVRHRPMDASELDTKFYGLVTPRFGEEKARRLERLLKRLDAAASVKDLM
                     AELEGAN"
BASE COUNT          662 a         1393 c         1266 g          787 t
ORIGIN      
        1 tgttccttgg tgttgtcgag cagtcccacc accttacccg tgagatcctt cgggcgcggc
       61 gccagcgcga ttttcgcgct gcccccgccg gcggtggggt cgatgaatcc agtttcgctc
      121 gtcatactgt tccttcctca caaagaagtg ataagtgaca ggtgataagt gataagactc
      181 ccgcgctgcg cgcggcagac tttttcaact cctatcacct atcacttatc acgtctcata
      241 cctcgtaagc gccgtgaacg gcgcggctcg tttcgttcca gccgtggcac accgcggtga
      301 tcggcccggt gccgcccgcg acgatgagat gtagccgctg cgggtccttg accgcccgga
      361 acggcgcgtt ttcgtcgtcg ggatcgatgc ccatcgcgcg cgcgcgttcg ggccgccagt
      421 tgccgccgcg cttgagttct gactgccggc gcaccgcgag ctcgctcagg agcttgacta
      481 cgtccttacg atccatcccc gctcgagcgc acagtcgcgc gtggtccggg ctcatcgcca
      541 cgaccatgtc ggagacgaac cacatgttcc aggagcccgg tgcggtcatc gcgtccccga
      601 tgccggtcaa gagccgctgc ggctcggtgc tcgcgtcgtc gaagtgcagc cggggatttt
      661 cggccatggc gcaggtaatc acgttgtcct ccggcgagta gccccgtgac gccgcgaggc
      721 tttcccacgg gctcaacgcc atgttttcgg tgaggcaggc ggtatagcgt atgggcgagc
      781 tgaacaccgt cgcggaggcg cggcccgaga tgccgccgcc cagattcagc aggatcagcc
      841 ggatcgcgcg gccgatggtg gcgttggcgc ggaaccccgg cccgaaacag ccgttgccgc
      901 cgtgcaggcc tattttctgc gcataaggac cattgacgat catgagtggg gcaacaccgt
      961 gcatcgtgca ctgcacgccg cccatgttga attcctcgcg caggatgagc ttcacgcccc
     1021 cgagcaccac gggaagatac tccggtttgc atcccgccat cagtgcgtgg atggcgaccg
     1081 cgcgcacggt ggcgacttcc atggccggcg gaatggaacc caccacctcg tcgggatcgc
     1141 gatccgtgcc ggcgagcatc ttccgcacgc gtgcctcggt cggcgtgacg actgggaacc
     1201 cgtccgacca gtccttctgc aaaaagaact cgaattcgtc gactttcgcc actacatcat
     1261 tcctcttggc aatttattcc ttatcggcgt ctatcggcgt tcatcccgtt ccaatgacct
     1321 tcggcagccc ctcacaattg atagtccgcg gtatgcccgc acaagggaca ttggaacggg
     1381 attcatcggc ggcttccttt ttcttacagc gcgaagtcga gcgcgctgaa atccgccacc
     1441 ttctccgcct ccgcgagccg ggaaaacagc cgctccgcct tcgcccggtc gcggtgcgcg
     1501 gtgagcttga ggaacttgtc gcgcagctcg tcgcgcgtca gcggcatcgt cggcgtgccc
     1561 ttgaagtcgt gcccctcggc ggaaacctcc ttgccgttct tcagcttcag cctcacccgg
     1621 cacgccggcg actggtggcc ttccgacttc ttgtacagct cgacctgcgc gcctttgcag
     1681 agcgcgcgga ttctcgcgtc gccgagagtc gcctcggaaa atacccgtgg atcgagcggg
     1741 tccttgtaga gcgcgatcgc gacgcagaat ggcgcgctgt actgcatcat ggtgaggtcc
     1801 cgcggctcgt tgatggcgtg ctgggtcacc gtcttctcgc tggcggcgat ggagattcgc
     1861 gcgacgtcgt cgcctgatat gccgtgcttc gccttcagac cgagcgccag cgtcaccggc
     1921 acctgcgcgg tcgcgtggca ggcgtagcgt ttgatcttgg tcttcatcac gtgccactcc
     1981 gagcccagat tcttcgtgaa acgggtcgca tcgccgtcgg tgcagaacac gttgaggaag
     2041 ccgaacttgc cgtcgagcgc gcctccggga ccggtgtagc cgtcgcgcgc gagcactgcg
     2101 gccatgaagc cgccctccgc cgcgcgtccg aggtgcaggc gcttgaccat gccgcccgtc
     2161 ctggagaatt ccatcagccc ggagctcatc gagcccgcga tgccgaaggc gttcgccatc
     2221 tgcttcgcgt ccagcccgaa catcctgccg accacgacgg cggtgccgaa cggccccacc
     2281 acccccggag aatggaagcc gttcttctcg atcgagtgcc tcgccgcgtc gccgatgcgg
     2341 tagagcacct cgtagccggc gacgatcgcg gaaagcagtg tcgtgccgct catcttgcgt
     2401 ccttgcgcgg gagccagacc cggcacggcg agcgcggcgc tgggatggat gccgacgctc
     2461 ggctcgcaca aagcatcgag ctcgaaggcg tgggccgacg cgccgttggt gaggcaggcg
     2521 aacggcgcgc gcaccttgat cggggtgccg aacacgctgc attcgcccgg cgcgctgttg
     2581 cgcttggcgt actcgatgac ggtttgggtc cagggcagct gcgatccaaa gacgcaggcg
     2641 gccaccgtgt cgattacgca atccttcgcg cgctcgatga cttcgcccgg gatgcggtcg
     2701 taggtcaggc cgacggcgaa ctcggccagg tgatgacttg cggtctgcat ttcagcggtt
     2761 gacctccagt ttcgattgct tgatcacctt ggaccacttg gcgatctccg acttgatcag
     2821 agcgccaaat gcctgtggcg cgccgcctgc cacctcggat ccgcctgcca ggaagaactg
     2881 cttcgccttc ggctgatcga ggatgacgtt gacttcccgg ttgattcgtt cgatgaaaac
     2941 cttcggcacg ccgcgcggcg ccagcatgcc gctccattgt atgacttcgt actgcgtaac
     3001 gccggcctcc cgcatcgtcg gaacatccgg catgccttcc gtgcgtttgg cggtgcttac
     3061 gcccaatgcg cgcagccgac ccgcccgcac atcactcgcc gtcgccagca tgttgccgaa
     3121 attgacctgc acctgtccgc cgatcagcgc ggtgcgcgcc ggcccgccgc cccgataggg
     3181 cacgtgcacc atgtccgtcc cggtgaggag cttgaacagc tcaccggcga gatggctgat
     3241 cgtgccttcg cccgacgacg cgaaattgat tttgcccggc tgcgctttcg cgagattgat
     3301 caagtcctga accgtccgcg ccggcacggc gggatgcacg accaggatca gcggaacatt
     3361 ggagatgtgg accacggggc cgaaatccct gatcgggtcg aaattgacct tgttgacgag
     3421 cgggttgatg acgaccgtgc tggcggtcgc catcaacaac gtatagccgt ccggagccgc
     3481 ctgggcggcg agctcggtgc cgatcgcgcc accggcgcca ccccggttgt cgacgaccac
     3541 cgtctggcca aggcgctcgg taagggtctg gcctacaaag cgcgccgcga catcggcgcc
     3601 gccccccgga ggaaacggct ggatcaggcg gatcggtcgg tacgggtagc gctcagccgg
     3661 tgcccggcac caggccgccg acgtcgcagc acccaggaac gcagccgcga ccaccgttac
     3721 aaccctgtgc attttgcaat ccatctccag tctctgcact gtctatctac tgtaggctca
     3781 attggcgcct tccagttccg ccatcagatc cttgacgctt gccgctgcat cgagccgctt
     3841 caacagacgc tcgagccgcc gcgctttctc ttccccgaag cggggcgtca ccaggccgta
     3901 aaacttcgtg tcgagttctg acgcgtccat cggccggtgc cgcacttcct cggtcaacgt
     3961 ctgccctgcg cgcgtcttga cggtcacgat cgcgcggtgg ccggcttgca gatccgtcca
     4021 gtcttcgcgg ccgacgaact tgacgcgctc ctgcatgtcg cgcgccgcgg catccatcgc
     4081 cccggcggga tcgtgcagcc ggtagaaa
//
LOCUS       DBNGWZ010000131         4862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2405588, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000131 DBNGWZ010000000
VERSION     DBNGWZ010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2405588"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /gene="lpdA"
                     /locus_tag="AC16CX_03925"
     CDS             <1..1689
                     /gene="lpdA"
                     /locus_tag="AC16CX_03925"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3011694.1"
                     /translation="LITLESDKATMDVPAPQAGVVKELKVKTGDKVSQGSLILMLEAS
                     DAAAAPAAKAPSAPAPTPAPEMAKAPMETKPATPPPAAAPVTPYAGPKGDIHAEVLVL
                     GAGPGGYTAAFRAADLGKKVVLVERFPTLGGVCLNVGCIPSKALLHIARVLTEAGEVA
                     HAGIEFGKPRVSLEKLRAWKAGVVGKLTKGLAGLAKQRKVQVVQGKGRFVSSHALRVE
                     SPQGIKTISFDHCIIAAGSSIARIPGFPYDDRRMIDSTSALEIPEVPKRLLVIGGGII
                     GLEMATVYDALGSKITVVELMDSLIPGADKDVVRPLAKRIEKRYEKILLKTKVAKVDA
                     QKDGLKVTYEGDGAPRPEMFDTILMAVGRRPNGKDIDAQAAGVNVNERGYIPVDKQLR
                     TNVPHIFAIGDVCGEPMLAHKATHEGKTAAEVIAGHKAYFDARTIPSVAYTDPEVAWM
                     GLTETEAQAKGIQYEKAAFPWAASGRALSMDRDEGMTKLLFDRNSRRLLGAAMVGVNA
                     GELISETVLALEMGADATDIGLTVHPHPTLSETVFFAAEIAEGSITDLYMPKKP"
     gene            1880..2071
                     /locus_tag="AC16CX_03930"
     CDS             1880..2071
                     /locus_tag="AC16CX_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011695.1"
                     /translation="MATLHLPKRQPLRKVENPREKLDRQLDEGLKQAFPTSDPVAITT
                     PGGPVAADEDPNQPKSGRS"
     gene            complement(2177..3175)
                     /locus_tag="AC16CX_03935"
     CDS             complement(2177..3175)
                     /locus_tag="AC16CX_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011696.1"
                     /translation="MMKKTGLLAAVVLAVLAVALPAQAQDLPEVKVITFRNGNNWPIW
                     AAQERGDFAKNGIRIALAYTPSSKTLIAGLVEGKFDIAIAAIDNMVAYQEGQSEVKFE
                     QEVDLVAVMGVNNGFNSLMAAPHVKNVADLRGRKVAVDAPGTAYALVLYDILRRNGLR
                     SGDYVIEVAGGANRRWEALRENKQDATLLSLPWDIIAAGNGFKRLATSNDIGRYQGSA
                     GIVQRKWAIENEARLVGFIRAYVAGYDWIANPANRDEAIALLRKNIKSLTADLAEESY
                     RALLDPATGLQRRAEFDVAGLRTVLALRGEYGKPQKKLTDPMKYYDPSYYKKALAR"
     gene            3397..>4862
                     /locus_tag="AC16CX_03940"
     CDS             3397..>4862
                     /locus_tag="AC16CX_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011697.1"
                     /translation="MLDWLSNVGKKADHPMYNVEEARKLLRDLPADQCKALEEVSSWL
                     ETVAAAEGYAPDDRIGVVKLLDETGQDYEPVVLEHFLRKAGLKEFDRLKLWQALVAFW
                     EHLSAAYRRCLRDIGQPAGSGAAHPECALLLSRALRALASEAKVLHLRYLPAQPRAWK
                     ELADLYSLGEKAQLGEESLKAYATDAVLTGPRREFLKAMMLEAASPESESSAAVELSA
                     RIIARFASGFILEAKPGPGCNLCFDLARPDRPIHYSPKIPARDTLRYLGAGRATAAIQ
                     EAIDGYTARPEETDRRFGEEYSVGDKLMTMKRLLLHWGDSSPRRRGPRVKIDAKVKVS
                     RGFESVAELATRIEFSGMAEMTEDQRLKVKQQTGIALQAEEATAVITEWMERDGSAWG
                     IGVDIPRQDEPWAKVGTLVALQPPAQKAWWVGAIQRMHRDQHDRPHAGIEILAKKPLS
                     VYLRGVGEGAQRAENWQTSSGSFQFTYLNAIILGESATT"
BASE COUNT          902 a         1568 c         1567 g          825 t
ORIGIN      
        1 ctgatcacgc tggaatcgga caaggcgacg atggacgtgc ccgcgccaca ggccggcgtg
       61 gtgaaggagc tcaaggtcaa gaccggcgac aaagtttcgc agggttcgct gatcctgatg
      121 ctggaggcga gcgatgcggc tgccgctccg gccgcgaaag cgccctccgc acccgcaccg
      181 accccggcgc ctgaaatggc gaaggcgccg atggaaacaa agcctgcgac acctccgccc
      241 gcagccgctc ccgtcacgcc ctatgccggc cccaagggcg acatccacgc cgaagtgctg
      301 gtgctgggag cggggccggg tgggtatacc gcggcgttcc gcgccgccga tctcggcaaa
      361 aaagtggtgc tggtcgagcg tttcccgacg ctcggcggcg tgtgcctgaa cgtgggctgc
      421 attccttcga aggcgctgct gcacatcgcc agggtgctga ccgaagccgg ggaggttgcg
      481 catgccggca tcgagttcgg caagcccagg gtgagcctgg agaagctgcg cgcctggaaa
      541 gcgggcgtcg tgggcaagct caccaagggg ctcgcgggcc tcgcgaagca gcgcaaggtc
      601 caggtcgtgc agggcaaggg gcggttcgtc tcgtcccatg ccttgcgggt cgaatccccg
      661 caaggcatca agacgatttc attcgatcat tgcattatcg ctgcgggttc gagcatcgca
      721 agaatccccg gctttccgta cgacgacagg cgcatgatcg actcgaccag cgcgctcgag
      781 attcccgaag tgccgaagcg gctgctggtc atcggtggcg gcatcatcgg gctggagatg
      841 gcgacggtgt acgacgcgct cggctcgaag atcacggtgg tggaactcat ggactcactg
      901 atccccgggg ccgacaagga cgtcgtgcgc ccgctcgcga agcgcatcga gaagcgctac
      961 gagaaaatcc tcttgaagac gaaagtcgcg aaggtcgacg cgcaaaaaga cgggttgaag
     1021 gtcacctacg aaggcgacgg cgcgccaaga cccgaaatgt tcgacaccat cctgatggcg
     1081 gttggccgcc ggccgaacgg aaaggatatc gacgcacagg ccgcgggcgt caatgtcaat
     1141 gagcgcggct acataccggt cgacaagcag ctccgcacca atgtgccgca cattttcgcg
     1201 atcggcgacg tgtgcggcga gcccatgctg gcgcacaagg cgacccacga aggcaagacc
     1261 gccgcggagg tgatcgccgg gcacaaggcg tacttcgacg cgcgcacgat accctcggtc
     1321 gcctataccg atcccgaggt cgcgtggatg ggattgaccg agaccgaagc ccaggcgaag
     1381 ggcattcagt acgagaaagc cgccttcccg tgggcggcga gcggccgcgc gctgtccatg
     1441 gaccgcgacg aaggcatgac gaagctcttg ttcgacagga acagccgccg attgctcggc
     1501 gcagcgatgg tgggggtcaa tgccggcgag ctgatctcgg aaaccgtgct cgcgctcgag
     1561 atgggcgcag atgcgaccga catcggcctg accgtccacc cgcacccgac gctttccgaa
     1621 accgtcttct tcgctgccga gatcgcggaa gggtcgatca ccgacctcta catgccgaaa
     1681 aagccgtgac tggtgactcg tgaatggtaa ccggttactg cgccttcagc gcacacctgt
     1741 gctcaggcgg ctgtccttcc actgactacc gactgtcgtg aatccacgac agtctccggc
     1801 aattcgggat cgaacccggc gtgcgctacg cggtcatact tccgcacgtc ccttttcgat
     1861 gaagtcggag aaattccata tggcaacgct acacttgccc aagcgtcagc ctctccggaa
     1921 agtggagaat ccccgggaaa agctggatcg ccaactcgat gaaggtttga agcaggcttt
     1981 tcctaccagc gacccggtgg cgatcaccac gcccggcggg ccggtcgcag cggacgagga
     2041 tccgaaccag ccgaagtcag gccggagcta gctccgcaac gcgcgccacg caagctggag
     2101 gttccgcgac gcagcatcac gttacgcatc agcacgccat gctgcatcac gtcacgccgt
     2161 atcacgtcac gccccatcaa cgagcgagcg ccttcttgta gtacgacggg tcgtagtatt
     2221 tcatcgggtc ggtgagcttc ttctgcggct tgccgtactc tccccgcagc gcgagcacgg
     2281 tgcgcaggcc cgcgacgtcg aattccgccc tgcgctgcag gccggtcgca gggtcgagca
     2341 gcgcacggta gctttcctcc gccagatcgg ccgttaagct cttgatgttc ttgcgtagca
     2401 acgcgatcgc ctcatcgcgg ttcgcgggat tggcgatcca gtcgtagcct gcgacgtagg
     2461 cgcggatgaa tccgactagt ctcgcctcgt tctcaatcgc ccacttgcgc tgcacgatgc
     2521 ccgcggagcc ctgatagcgg ccgatatcgt tcgaggtggc aagacgcttg aacccgttgc
     2581 ctgccgcgat gatgtcccaa ggcagggaga gcagtgttgc gtcctgcttg ttttcgcgca
     2641 aagcttccca ccgcctgttc gcgccgccgg cgacttcaat gacgtaatcg cccgagcgca
     2701 ggccgttgcg ccgcaagatg tcgtaaagta ccaaggcata ggcggtaccg ggagcgtcca
     2761 ccgcgacctt gcgtcccctg aggtcggcca cgttcttgac gtgcggcgcc gccatcaagc
     2821 tgttgaagcc gttgttgacc cccatgaccg cgacgagatc gacttcctgc tcgaatttca
     2881 cctcgctctg cccttcctgg taggcgacca tgttgtcgat cgcggcgatc gcgatgtcga
     2941 acttaccttc gaccaagccg gcgatcaggg tcttcgagct cggtgtgtag gccagcgcga
     3001 tgcggatacc gttcttcgcg aaatcgccgc gctcctgcgc cgcccagatt ggccagttat
     3061 ttccgttgcg gaaggtgatg actttcactt ccggcagatc ctgcgcctgc gcggggagcg
     3121 cgaccgcaag cacagccagc accacggcag ccagcaaccc tgttttcttc atcatgatgg
     3181 tctcctcttt cgacgcgatt ttagcgtttc atccccggag tttcgacggg caggccgttt
     3241 cagcgctttt tgtcacgaat tcgagccgga aagcagtctc gcccgcaaca acttttgtcc
     3301 tagatcaaaa agaccgcccg gtatcggctg gtagatttag gcgttatgat gacacccgtg
     3361 acccgcccgc cccgccacca gcgtgggagc ttgcaggtgc tcgactggct gagcaacgtc
     3421 ggcaagaagg ccgaccatcc gatgtacaac gtcgaggagg cgcgcaagct gctgcgcgac
     3481 ctccccgcgg accagtgcaa ggccctcgag gaagtctcct cgtggctgga aaccgtcgcc
     3541 gcggccgagg gctacgcgcc tgacgaccgc atcggcgtgg tgaagctcct cgacgagacc
     3601 ggtcaggatt atgaacccgt cgttctggag catttcctgc ggaaggcggg gttgaaggag
     3661 ttcgaccggc tcaagctctg gcaggcgctg gtcgcgttct gggagcacct ttccgccgcc
     3721 taccggcgct gcctgcggga catcgggcag ccggccggat ccggcgccgc acatccggaa
     3781 tgcgcgctgc tcctctcgcg cgcgctgcgt gccctggcca gcgaggcgaa ggtccttcat
     3841 ctgcgctact tgccggcgca gccgcgcgcc tggaaggagc tcgccgacct ctacagcctg
     3901 ggggagaagg cgcagcttgg cgaggaatcg ctgaaggcct acgccaccga cgccgtgctc
     3961 accggcccgc gccgcgaatt cctgaaggcg atgatgctgg aagccgcctc tcccgaatcc
     4021 gaatcatccg cggccgtgga gctctccgcg cgcatcattg cgcgcttcgc ctcgggtttc
     4081 atcctcgagg cgaagcccgg cccgggctgc aacttgtgtt tcgacctcgc gcgcccggac
     4141 cgcccgatcc actattcgcc gaagattccg gcgcgcgaca ccctgcgtta cctcggcgcg
     4201 ggccgggcaa ccgccgcgat ccaggaggcc attgacggct acaccgcccg cccggaagaa
     4261 acggacaggc gcttcgggga ggaatacagc gtcggcgaca agctgatgac catgaagcgc
     4321 ctgctgctcc actggggcga ctcgtcgcct cggcggcgcg gtccgcgcgt gaagatagac
     4381 gcgaaggtga aagtctcgcg cgggttcgag tccgtggcgg agctcgcgac gcggatcgag
     4441 ttcagcggga tggcggagat gaccgaggac cagcgcctga aggtgaaaca acagaccggg
     4501 atcgcgctcc aggcggagga ggcgaccgcc gtgatcacgg aatggatgga gcgcgacggc
     4561 agcgcgtggg gcatcggcgt ggacattccg cgccaggacg agccgtgggc gaaggtcggc
     4621 acgctggttg cgttgcagcc gccggcgcag aaggcatggt gggtgggtgc gatccagcga
     4681 atgcaccgcg atcagcacga ccggccgcac gccggcatcg aaatcctcgc caaaaagcca
     4741 ttgtcggttt acctgcgggg cgtcggcgag ggcgcgcagc gcgccgagaa ctggcagacc
     4801 tcttccggct cgttccagtt cacttacctg aacgcgatca tcctcggcga gagcgcgact
     4861 ac
//
LOCUS       DBNGWZ010000132         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_390240, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000132 DBNGWZ010000000
VERSION     DBNGWZ010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_390240"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="AC16CX_03945"
     CDS             <1..715
                     /locus_tag="AC16CX_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011698.1"
                     /translation="VTSASVQTLSIAGATAADFAPDSTRAFIVAGSTLYAFQQSGALR
                     PIPLSAGAVDVAFLASGAFAYLAGGAASAATARATCDNSLGDTVPTNGNPVLVRALPD
                     GSGMVAANSPGLDVITATTDRAGCPPVLGDTVVFKDFGQGAVTARQLIVLPNGAKVFV
                     TSGLGQLLVYDVAGGTTSAITLASGASAFTGGVKLDSSALYVGGSDNAVHRIDVSAGA
                     DAQQISVSFTPDLVAVRPK"
     gene            complement(829..1092)
                     /locus_tag="AC16CX_03950"
     CDS             complement(829..1092)
                     /locus_tag="AC16CX_03950"
                     /inference="COORDINATES: protein motif:HMM:NF022535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2621 family protein"
                     /protein_id="HIR3011699.1"
                     /translation="MNWSDDATSIVEELLRELPAPVRDAVKETAELRAEALTQDAGED
                     EVSMETAVSAFIESTPSDLRARLKHTLTYHGIDPEEYEAAFSS"
     gene            complement(1159..1581)
                     /gene="nusB"
                     /locus_tag="AC16CX_03955"
     CDS             complement(1159..1581)
                     /gene="nusB"
                     /locus_tag="AC16CX_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3011700.1"
                     /translation="MGTRRKSRELAMQMLFQADMGKQTPEQVRRTFWTERSDLDETVR
                     GFADDIFRAAHEHAEEINRLIESHAEHWRMERMAAVDRNLLRAAVAEFVGFPQTPKPV
                     VINEALEIARKFSTPESVQFINGVLDSIARELEKAGTA"
     gene            complement(1653..>1930)
                     /locus_tag="AC16CX_03960"
     CDS             complement(1653..>1930)
                     /locus_tag="AC16CX_03960"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3011701.1"
                     /translation="VARGIGQSAQETGVPHAFGVLTCDTLEQAIDRAGLKSGNKGFEA
                     ALAAVEMANLKQAVVSRRSPVVRGKQGSPKAGRSGSKRRKRAGARRS"
BASE COUNT          331 a          620 c          607 g          372 t
ORIGIN      
        1 ggtgaccagc gcgtcggtgc agaccttgag catcgcgggc gccacggcgg ctgattttgc
       61 gcccgacagc acgcgggcgt tcatcgtggc aggaagtacc ttgtacgcgt tccagcagag
      121 cggcgccttg cgtccgatcc ccctcagtgc gggggcagtg gacgtcgctt tcctcgcctc
      181 cggggcgttc gcttacctcg ccggaggtgc agcttcagcg gcgacggccc gcgccacttg
      241 cgacaactcc ttgggcgata ctgtgccgac caacggcaat cccgtcttgg tgcgggccct
      301 gccggatgga agcggcatgg tcgccgccaa ctcgccgggt ttagacgtga tcacggcaac
      361 aaccgaccgc gccggctgtc cgccggtact aggggatacc gtggtcttca aggactttgg
      421 ccagggagcc gtcactgcgc ggcagttaat cgtgctgcca aatggagcga aggtgttcgt
      481 gaccagcggt ctcggtcagc tcctggtcta cgacgtcgcg ggcggaacaa cttcagccat
      541 cactctggcg agcggggctt ccgccttcac cggcggggta aagctggaca gctcggcgct
      601 ctacgtgggt ggcagcgaca acgccgtcca tcgcattgat gtgagcgctg gcgcagacgc
      661 gcagcagatc agtgtctcgt tcaccccaga cctggtggcg gtgaggccga agtgaccgtg
      721 ttttcgcatg catcagccga ggcgcaaagc gccgagccct gagcgcagtc gaagggccgc
      781 gcggtcatcc tgaggcgaag ccgaaggatc tttgtctacg cgaaatcctc atgaagaaaa
      841 cgctgcctca tattcctccg gatcaatccc gtgataggtg agcgtgtgtt tcaggcgtgc
      901 gcgcaggtcg gagggtgtgg actcgatgaa agcgctcacc gcagtctcca tggaaacctc
      961 gtcttcgccc gcatcctggg tcagcgcttc ggcgcgcaac tcggcggttt ccttcactgc
     1021 gtcgcgcacc ggcgccggca actcgcgcaa gagctcttct acaatcgaag tagcgtcgtc
     1081 ggaccagttc ataagcagta gtcaggagtc agtagctagc agtctgtggt tagcagctca
     1141 aatcaagaat acatcccact aggcagtgcc cgccttttcc agttcccggg cgatgctgtc
     1201 cagcacgccg ttgatgaact gcaccgattc cggcgtggaa aacttgcgcg cgatctccag
     1261 cgcttcattg atgaccaccg gcttcggagt ctggggaaag ccgacgaact ccgccaccgc
     1321 ggcgcgcagc aggttgcggt ccacggcggc catgcgctcc atgcgccagt gctcggcgtg
     1381 cgactcgata agccggttaa tttcctcggc gtgctcgtga gcggcccgga agatgtcgtc
     1441 cgcgaagccg cgcacggtct cgtccaagtc ggagcgctcc gtccagaagg tgcggcgcac
     1501 ctgctccggc gtctgctttc ccatgtccgc ctgaaagagc atctgcatcg ccagctcgcg
     1561 ggatttgcga cgcgtgccca tgaaaagcag tagctagtag tcagtagtca gttagttgtg
     1621 atgccttgca gtcttccgca aggcccgaga tgtcaggaac ggcgtgcgcc cgcacgcttc
     1681 cttcgcttgg atcctgatct accggctttc ggcgagccct gcttgcccct gacgaccggc
     1741 gaccgacgac tgacgacggc ctgcttcaga ttcgccatct ccaccgccgc cagcgccgcc
     1801 tcaaagcctt tgttgccgct cttcaggccg gcgcggtcaa tggcctgttc cagcgtgtcg
     1861 caggtaagca cgccgaaggc atggggcacg ccggtttcct gggccgactg gccgatgccg
     1921 cgcgccactt
//
LOCUS       DBNGWZ010000133        10771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_138649, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000133 DBNGWZ010000000
VERSION     DBNGWZ010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_138649"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="AC16CX_03965"
     CDS             complement(<1..1430)
                     /locus_tag="AC16CX_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HIR3011702.1"
                     /translation="MEILFLLGAPLAGGALLAMFGGRRQAPELGVGASLVTFLAAVAL
                     TARVIRDGPITAFESQFFIDSFNVVLVALTSFVAFTTALFSRPYMRIEADRGKVKAGA
                     LKMYHAMFQVFTFTMLLALTTNNMGILWVAMEAATLSTVLLVSLYRTPEAIEAAWKYF
                     ILCSVGIAQALFGTMLLYFAAEKVLGSGGTALLWTDLNQIKDQLEPTVMSLAFVFLLV
                     GYGTKVGLVPVHNWLPDAHAEGPTPISAVLSGLLLNVALYAVVRCKVLTDGALGAPFA
                     GNLMMGFGLLSVVVAAFLLSRQRDIKRMFAYSSIEHMGLMTFAFGMGGVAATFGALLH
                     MTMHSLTKSAIFFAVGHAAQKSGTQIMDDIRGLIRTNPTIGWGLMLGSIAILGIPPFG
                     VFASEFIIITTAMREQPWATPFLLVALGVAFAAIFGKVQPMVFGETQLPRLPHPPALI
                     PVFVHLALVLMLGIFIPPYLAAWYRQA"
     gene            complement(1430..2086)
                     /locus_tag="AC16CX_03970"
     CDS             complement(1430..2086)
                     /locus_tag="AC16CX_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HIR3011703.1"
                     /translation="MPLSGQIINVLAAIMLLIAFAMLSQRRILSLIELFAWQGVVLSL
                     STFIVAYTTGQGHLVFSGILTLLLKVIALPWILYRLIHRINIRWDVETLINIPTTMLI
                     GIALVIFAFNLAQPISQMASTVTRSTLGIAMACVLLSFLMMITRRKAVPVVIGFLAME
                     NGLFFAATSATYGMPMVVELGIALDVLVGAFIFGIFFFQIRETFESLDVRHMEKLKED
                     "
     gene            complement(2130..3077)
                     /locus_tag="AC16CX_03975"
     CDS             complement(2130..3077)
                     /locus_tag="AC16CX_03975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR3011704.1"
                     /translation="MTFGGALNQLVQLLLVAAAAPLLVGWVNACRAWLQNRSAPSLLQ
                     PYRMLRKLLHKDAALAENASWLFRFAPYVIFGCMWLVAGIVPVLATDLPFATAADVIA
                     LVGVFAVARVFISLAAMDIGTAFGTLGARREMLIGALAEPALLIIFFNFSLISQSTSL
                     TTIVETLAHREFALYPSLAFAAVAFVMVLLAENARIPVDNPTTHLELTMVHEALVLEY
                     SARHLALIEWASAIKLFAYCAVGIACFLPWGIAEAGDWDALPLAVIAAVAKLVVAGAG
                     LAVLETVSAKMRIFRVPEFVATAFMLAMLGLLLHYVFEV"
     gene            complement(3074..5086)
                     /gene="hyfB"
                     /locus_tag="AC16CX_03980"
     CDS             complement(3074..5086)
                     /gene="hyfB"
                     /locus_tag="AC16CX_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HIR3011705.1"
                     /translation="MPAAIASPLEIVIGIVLFWLAIGLLGVLRPNQPDTITRFLYPLG
                     ALAGVVLAVVGLVAIGLPWKGMVLPLGLPDLPFHVRLDALSAFFLLLLGATSAGISCY
                     AAGYFAGTEDGSAGLTCLQYHWFLASMALVLIADDAYLFMVAWEAMALCSYFLVTTEH
                     HDPEIRKAGFTYLLMAHVGAIAILLCFGVLHGAHGDYTFDAMRQAQLPVGWATVAFLL
                     ALLGFGAKAGIVPLHVWLPEAHPAAPSPVSALLSGVMLKTAIYGLFRVTYDLLPAIQW
                     EWGLLVLVIAVVTTLFGVLFALMQHDLKRLLAYHSVENIGIILIGFGLSLIYFGTGHP
                     VLAVLGLLAALYHALNHAVFKGLLFLGAGAVLRGSGLRDLGRMGGLIRVMPYTAFFFL
                     VGSLAISALPPLNGFVSEWLTFQAALLSPALEHGVLRSIIPLAAAALALAGALTAMCF
                     VKVFGVAFLGLPRSDQAVRGREIGGWEQLGMAWLATLCVLLGLSPVWMIGVMDPIALG
                     LLDYSLAYSGAASHWLWLTPVAPERASYSPVIFFAVIVAVVLAAFGLVRVLYHGRLRR
                     TPAWDCGFPGLNARMQDTAEGFAQPIRQIFAPVYEIESHHPDPFDRLPRYFSEVHDRF
                     WGWIYFPIARAATFCSRLVGRIQVGRVAIYLMYSFVTILFLLAFVR"
     gene            complement(5471..5926)
                     /locus_tag="AC16CX_03985"
     CDS             complement(5471..5926)
                     /locus_tag="AC16CX_03985"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3011706.1"
                     /translation="MNKASRNITLGTALLIAVAGCATTGGGDPKANVAPDKVVYHIND
                     TGAQAVAALRNIGNHLEVNPKAHIVVVTHAQGVDFLMEGAKDRNGNPYNIRVEELKSQ
                     GVKFDVCEITLRNRKMTRTQFIPEATFVPSGVAEITRLQQREGYAYLRP"
     gene            6070..6348
                     /locus_tag="AC16CX_03990"
     CDS             6070..6348
                     /locus_tag="AC16CX_03990"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3011707.1"
                     /translation="MAQVTKLLRISGRVQGVAFRFYMERKARELGVTGWVRNCRDGSV
                     EALVQGAPEAVEAMIAWARRGPPSAVVGDFKVSEAGGDFESFEVRPSA"
     gene            complement(6617..>10771)
                     /locus_tag="AC16CX_03995"
     CDS             complement(6617..>10771)
                     /locus_tag="AC16CX_03995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3011708.1"
                     /translation="GDETRVVYVSPLKALSNDIHKNLEEPLAGIRKELAALGHPDVEI
                     RTLVRTGDTPQAERERMRRKPPHILVTTPESLYILLTSESGRRALATTRTVIVDEIHA
                     LAPNKRGAHLALSLERLEHVAGAKLTRVGLSATQKPIEEVASFLTGGRNRPAAGLFDD
                     RTAGSGCTIVDTGHARKRDLALEAPESPLEAVMSAEVWEQVYDRLAELVREHRSTLVF
                     VNTRRLAERAARALTERLGEGEVTSHHGSLAREQRLNAEQRLKRGELKALVATSSLEL
                     GIDIGHVDLVCQLGTPRAISALLQRVGRSGHAVGGLPKGRLFPLSRDDLVECAALLDA
                     VRRGELDRLRIPQKPLDVLAQQIAAEVACGEWDEDALFDLLRHAWPYRGLEREEYGAV
                     VQMLSEGYATRRGRSRAVVHRDAVNRKLRPRQGLRLTAVTCGGTIPDTADYAVVLAPE
                     EQVIGTVNEDFAVESMAGDIFQLGNTSYRILRVEAGRVRVEDAAGLPPSIPFWLGEAP
                     ARTDEVSAAVSRLRGDLETRLGDISAALAHLTEEIGIAAEAAQQIVDYFAAAKAVLGL
                     LPTQGHLVFERFFDETGGAQLVIHSPYGNRVNRAWGLALRKRFCRKFNFELQAAATED
                     AIILSLTSTHSFPLEEVARYLNADTVCEVLTQALLDAPMFMTRWRWNASIALALLRFR
                     GGNKVAPQLQRMEAEDLIAAIFPDQLACQENIAGDREIPDHPLVNQTISDCLHEAMDV
                     EGLERLLRSLERGEVQVTARDLVEPSPLAQEILSARPYAFLDDAPLEERRTQAVVSRR
                     WLDPESAADIGRLDPEAIVRVKEEAWPAADNADELHDALVWLGLLTQEEVRAQPAWSG
                     LLQELALSKRAARIIPATPGANGFWIAAERLPEFHAVHPGSRVEPEISAPAEFAQKDW
                     TREAALVEIVRGRLEGLGPVTAATLAADIEVPVADVEIALAALESEGFAMRGKFTVDA
                     ASGEWCERRLLARIHRYTLKRLRREVEPVAAADFMRFLFDWQRVATDERMEGPDAVAA
                     VVSQLEGFEAAAAAWETALLPARVAGYEPAWLDDLCLAGRVLWTRLNAPRVNAERAQG
                     PAPVRGTPVTLVTRKNLPVWTALVKPATAADLRLSSRAQAVADYLSQHGASFFDDIVA
                     GLNLPRTFVEDALRELVAAGLTNSDGFSGLRALLLPADRRKPVSGGRRRRTALFGVED
                     AGRWALIRRKPAVAVESRLPREVVEQIVRTLLRRYGVVFWRLLAREADWLPPWRDLLM
                     ACRRLEARGEIRGGRFVAGFSGEQFALPEAVGVLRSVRNEEKTGRLTVVNGADPLNLA
                     GVIVPGAKVPALHGNRVLYRDGVAVAALVAGEAQYFERVEPEQAWEMKNLLLRTAAPH
                     ELAALM"
BASE COUNT         2022 a         3569 c         3552 g         1628 t
ORIGIN      
        1 gcctgccggt accacgccgc aagatacggc gggatgaaga tccccagcat cagtaccagc
       61 gccagatgca cgaacaccgg gatcagcgcc ggcgggtgcg gcagccgcgg caactgcgtc
      121 tcgccgaaca ccatcggctg caccttgccg aagatggcgg cgaaggccac gccgagcgcc
      181 accagcagga acggcgtcgc ccacggctgc tcgcgcatcg cggtggtgat aatgatgaac
      241 tcgctcgcga acacgccgaa cggcgggatg ccgaggatcg cgatcgagcc cagcatcagt
      301 ccccagccga tcgtcgggtt ggtccggatg aggccgcgga tgtcatccat gatctgcgtg
      361 ccgctcttct gcgccgcgtg cccgaccgcg aagaagatcg ccgacttggt gaggctgtgc
      421 atggtcatgt gcagcagcgc gccgaacgtc gccgccaccc cgcccatccc gaaggcgaac
      481 gtcatcaacc ccatgtgctc gatcgaggaa taggcgaaca tgcgcttgat gtcgcgctgg
      541 cgcgagagca ggaacgcggc caccactacc gacagcagcc cgaagcccat catgaggttg
      601 cccgcgaacg gcgcgccgag cgcgccgtcg gtgagcacct tgcagcgcac gaccgcgtag
      661 agcgcgacgt tgagcagcag gcccgagagc accgccgaga tcggcgtggg gccttccgcg
      721 tgcgcgtcgg gcagccagtt gtgcaccggc accagcccca ccttggtgcc gtagcccacc
      781 agcagaaaca cgaaagcgag cgacatcacc gtcggttcga gctggtcctt gatctggttc
      841 aaatcggtcc acagcagcgc cgtgccgccc gatcccagca ccttctccgc cgcgaaatag
      901 agcagcatgg tgccgaacag cgcctgcgcg atgccgacag agcacaggat gaagtacttc
      961 cacgccgcct cgatggcttc gggcgtgcgg tagaggctca ccagcagcac cgtcgagagc
     1021 gtcgccgctt ccatcgccac ccacaggatg cccatgttgt tcgtcgtcag cgcgagcagc
     1081 atggtgaacg tgaagacctg gaacatggcg tgatacatct tcagcgcccc cgctttcacc
     1141 ttgccgcggt cggcctcgat gcgcatgtac ggacgcgaga acagcgccgt ggtgaaagcg
     1201 acgaaggaag tgagcgcgac cagcacgaca ttgaatgaat cgatgaagaa ctggctctcg
     1261 aacgcggtaa tgggaccgtc gcggatcacg cgcgcggtca gcgccaccgc cgcgaggaag
     1321 gtgacgaggc tcgcgccgac gcccagctcc ggcgcctggc gccggcctcc gaacatcgcc
     1381 agcagagcgc cgccagcgag cggcgccccg agcaagaaca gtatttccat caatcttcct
     1441 tcagcttctc catgtgccgc acgtccaggc tctcgaaggt ctcgcggatc tggaagaaga
     1501 agatgccgaa gatgaacgcg cccaccagca cgtcgagcgc gatgccgagc tccaccacca
     1561 tcggcatgcc gtaggtcgcg ctggtcgccg cgaagaacag gccgttctcc atcgcgagga
     1621 agccgatcac gaccggcacc gccttgcgcc gcgtgatcat catcaggaac gagagcagca
     1681 cgcacgccat ggcgatgccg agcgtcgagc gcgtgacggt gctcgccatc tgcgagatcg
     1741 gctgcgccag attgaaggcg aaaatcacga gcgcaatgcc gatcagcatg gtagtcggga
     1801 tgttgatgag cgtctcgacg tcccagcgga tgttgatgcg gtggatcaac cggtaaagga
     1861 tccacggcag cgcgatcacc ttcagcaaca gcgtcaggat gccggagaaa accaggtgac
     1921 cctgtcccgt cgtgtaggcg acgatgaagg tggagagcga cagcaccacg ccctgccacg
     1981 cgaacagctc gatcagcgac aggatgcgcc gttgcgacag catggcgaac gcgatcagca
     2041 gcatgatcgc cgcgagcaca ttgatgattt gtccgctcaa tggcataaga gcgtgatggg
     2101 tgatgggtga tgggtgatgg gacgcattct tacacctcga agacgtaatg cagcagcagg
     2161 ccgagcatcg cgagcatgaa ggcggtagcg acgaattccg gcacgcggaa gatgcgcatc
     2221 ttcgcgctca ccgtctccag caccgcgagg cccgctcccg cgaccacgag cttcgccacc
     2281 gccgcgatga cggcgagcgg cagcgcgtcc cagtcgccgg cttccgcgat gccccacggc
     2341 aggaaacagg cgattccgac cgcgcagtag gcgaacagct tgatcgcgga ggcccactcg
     2401 atcagcgcga ggtgccgggc ggaatactcg agcaccagcg cctcgtgcac catggtgagc
     2461 tcgaggtggg tggtgggatt gtccaccggg atgcgcgcgt tctcggcgag cagcaccatc
     2521 acgaacgcca cggcggcgaa ggcgaggctg ggatagagcg cgaattcgcg atgggccagc
     2581 gtctcgacga tggtggtgag cgaagtggac tggctgatca gcgagaaatt gaaaaagatg
     2641 atgagtaggg ccggctccgc cagcgcgccg atcagcatct cgcggcgggc gcccagcgtg
     2701 ccgaaagccg tgccgatgtc catcgccgcg agcgagatga agacgcgcgc cacggcgaac
     2761 acgccgacca gcgcgatcac gtcggcggcc gtcgcgaacg gcaggtcggt cgccagcacc
     2821 ggcacgatgc cggccacgag ccacatgcag ccgaagatga cgtagggcgc gaagcggaac
     2881 agccaggagg cgttttccgc gagcgccgca tccttgtgca ggagcttgcg cagcatgcgg
     2941 tacggctgaa gcaggctcgg cgcgctccgg ttctgcagcc aggcgcggca ggcgttgacc
     3001 cagcccacga gcagcggtgc ggcggccgcc accagcagca gctgcaccaa ttgattgagg
     3061 gcgccgccga aggtcatctc acgaacgcca gcagaaaaag gatggtcacg aagctgtaca
     3121 tcaggtaaat ggcgacccgc ccgacctgga tccgcccgac aaggcgcgag cagaacgtgg
     3181 cggcgcgcgc aatcggaaag tagatccagc cccagaaacg gtcatgcacc tcggagaaat
     3241 agcgcggcag gcggtcgaac ggatcgggat ggtgggactc gatctcgtac accggcgcga
     3301 agatctggcg gatcggctgg gcgaaccctt ccgccgtgtc ctgcatgcgg gcgttcaggc
     3361 ccgggaagcc gcaatcccac gccggcgtgc ggcgcagccg gccgtgatag agcacgcgca
     3421 ccagcccgaa cgcggccagc accacggcga cgatcacggc gaagaagatc accgggctgt
     3481 agctcgcgcg ctcgggcgcg accggcgtca gccacagcca gtgcgacgcg gcgcccgagt
     3541 aggcgaggct gtaatcgagc aggccgaggg cgatgggatc catcaccccg atcatccaca
     3601 ccggcgagag gccgaggagc acgcacagcg tggcaagcca tgccatgccg agctgttccc
     3661 agccgccgat ctcgcggccg cggaccgcct gatcgctccg cggcaggccg aggaaggcca
     3721 cgccgaacac tttcacgaag cacatcgcgg tgagcgcgcc ggcgagcgcg agcgccgcgg
     3781 cggcgagcgg gatgatcgag cgcagcacgc cgtgctccag cgccggcgag agcagcgcgg
     3841 cctggaacgt gagccattcg gaaacgaagc cgttcaacgg cggcagcgcc gaaatcgcga
     3901 ggctgcccac caggaagaag aacgcggtgt atggcatgac gcggatcagg ccgcccatgc
     3961 gaccgaggtc acgcaggccg gagccgcgca ggaccgcgcc tgcgccgagg aacagcagtc
     4021 ccttgaatac ggcgtgattg agcgcgtgat agagcgccgc gaggaggccg agcacggcga
     4081 gcaccgggtg gccggtgccg aaatagatca acgacagccc gaagccgatc aggatgatgc
     4141 cgatgttctc gaccgagtgg taggcgagca ggcgcttgag atcgtgctgc atcagcgcga
     4201 acaggacgcc gaagagcgtg gtgaccacgg cgatcaccag caccagcagt ccccattccc
     4261 actggatcgc cggcagcagg tcgtaggtga cgcggaacag gccgtagatc gcggtcttca
     4321 gcatcacccc ggagagcagt gcggacaccg gcgagggcgc cgccgggtgc gcctccggca
     4381 accagacgtg cagtggcacg atgccggcct tggcaccgaa cccgaggagc gcgagcagaa
     4441 acgcgaccgt cgcccacccc accggcagct gggcctgcct catggcgtcg aaggtgtagt
     4501 cgccgtgcgc gccgtgcaaa acgccgaagc agagcaggat cgcgatcgcg cccacgtgcg
     4561 ccatcagcag ataggtgaag cccgctttgc ggatctccgg atcgtggtgc tcggtggtga
     4621 cgaggaagta ggagcagagc gccatcgcct cccacgccac catgaagaga taggcgtcgt
     4681 cggcaatgag caccagcgcc atgctggcga ggaaccagtg atattgcagg caggtgaggc
     4741 cggcggagcc gtcctccgta cccgcgaaat agccggcggc gtagcatgaa attccggcgg
     4801 aggtggcgcc gagcagcagc aggaagaacg cggagagcgc atcgagccgc acgtggaacg
     4861 gcaggtccgg cagcccgagc ggcagcacca ttcctttcca cggcaggcca atcgcgacca
     4921 gcccgacgac cgcgagaacc acgccggcta acgcgccaag ggggtagaga aagcgcgtga
     4981 tggtatccgg ctggttcggg cgcaggaccc caagcagtcc gatcgccagc cagaacagca
     5041 cgatgccgat gacgatttcc aggggggacg cgatggcggc gggcatcggc tatgagcatg
     5101 acgcgcggat gggcggcccg gccgcgcggt aaagtgcgga ttttatgcgc tacgccacct
     5161 tgtacgcaaa cctgttaccg ccaaggaaaa aatgctggac cacaaaggaa agacaagata
     5221 attgaaccgc caagacgcca agagggacaa ggcgagaata gaaccgcaga gccccggaga
     5281 gaaacaagac aactattaaa tcaagaaccg aaacctcaaa gattgaatgc gaaaaagaaa
     5341 cggccggcag atgtccggcc attttattgg ttctctccgt acctccgcgg tttaatatta
     5401 ttcttgattc tcttggcgtc ttggcggttc aaatattgtt tttttcttgg tgccgtggcg
     5461 gttcagctta ttacgggcgg agataggcgt aaccctcgcg ctgctgcagc cgggtgattt
     5521 ccgcgacgcc cgagggcacg aatgtcgcct ctgggatgaa ctgtgttctc gtcatcttgc
     5581 ggttgcgcag cgtgatctcg cacacgtcga acttgacgcc ctgcgacttc aactcctcca
     5641 cgcggatgtt gtagggattg ccgttgcgat ccttggcacc ctccatcagg aaatccacgc
     5701 cctgcgcgtg cgtgaccact acgatgtgcg ccttcgggtt aacctccagg tgattgccga
     5761 tgttgcgcag tgcggccacc gcctgcgcgc cggtgtcgtt gatgtgatag acgaccttgt
     5821 cgggcgcgac attcgccttc ggatcgccgc cgccggtggt tgcgcaaccc gctaccgcga
     5881 taagcagcgc cgtcccgagc gtgatgttcc tggatgcctt gttcataatc ccctcctttg
     5941 gtgtatgaac cggttccggc tacacgttaa atcacaacgc ggcaaacggc aacgtttgcc
     6001 ctgccccgaa tataaaatga ccttgtccca agccaaggta atcgcggagt tgcctggatt
     6061 tccggtcgta tggctcaggt caccaagctg ctgaggatct ccggccgcgt gcagggcgtc
     6121 gctttccgtt tctatatgga gcggaaggcg cgcgagctcg gcgtcaccgg ctgggtgcgc
     6181 aactgccgcg acggcagcgt ggaggcgctg gtccagggcg cgccggaagc ggtggaagcg
     6241 atgatcgcat gggcgcgccg cggcccgcca agcgccgtgg tgggggactt caaggtgagc
     6301 gaggcaggcg gggacttcga gagcttcgaa gtccgcccca gcgcctaaaa accagaatcg
     6361 aaccgccaag acgccaagag aaacaaatac aaaaatagaa ccgagcatca aatcgtatcg
     6421 aaccaccgag ataaggagag aaccgagaac aacaatggaa atgaattcaa aacgagcggt
     6481 tttcgaaatt aatgcggttc ctgtcctcgc ctctctccat gacttctctg cggttcaatc
     6541 attgctctcg tttttcttgg cgtcttggcg gttcattatt cgtatcacgc tctcaggatt
     6601 tctcagcggt tcattcttac atcagcgcgg cgagttcgtg cggcgccgcg gtccggagca
     6661 gcaggttttt catctcccac gcctgctccg gttcgaccct ctcgaagtac tgcgcctcgc
     6721 cggcgacaag cgcggcgacc gcgacgccgt cgcggtagag cacgcggttg ccgtggagcg
     6781 ccggcacttt tgcgcccggc acgatgacgc cagcgagatt gagcgggtcg gcgccgttca
     6841 ccacggtgag ccgtcccgtc ttctcctcgt tgcgcacgct gcgcagcacg ccgaccgctt
     6901 cgggcagcgc gaactgctcg ccggagaagc cggcgacgaa gcgccctccg cggatctcgc
     6961 cgcgcgcctc cagccgccgg caggccatga ggagatcgcg ccacggcggc agccagtccg
     7021 cttcccgcgc gagcaggcgc cagaacacga cgccgtaacg gcgcagaagc gtgcgcacga
     7081 tctgctccac gacctcgcgc ggcaagcggc tttccacggc gaccgctggc ttacgccgga
     7141 tcaacgccca gcgcccggcg tcctcgacac caaatagcgc cgtgcggcgc cggcgcccgc
     7201 cgctgaccgg cttgcggcgg tccgcgggca acaacaacgc gcgcaagccg ctgaagccgt
     7261 ccgaattggt gagacccgcc gccaccagtt cgcgcagcgc gtcctcgacg aaagtgcgcg
     7321 gcaggttcag gcctgcgacg atgtcgtcga agaacgaggc gccatgttgc gacaggtagt
     7381 ccgccaccgc ctgcgcgcgc gagctcaagc gcaggtctgc ggcggtcgcg ggcttgacca
     7441 gcgcagtcca caccggcagg tttttgcgag tgactagggt gaccggcgtg ccgcgtaccg
     7501 gcgcgggacc ctgcgcgcgc tccgcgttca cccgcggcgc gttgagccgc gtccacagca
     7561 cccgccccgc aaggcacagg tcgtcgagcc atgccggttc gtaaccggcc acacgcgcgg
     7621 gcagcaacgc ggtttcccac gccgccgccg ccgcttcgaa cccctcgagc tgcgacacca
     7681 ccgccgcgac cgcgtccggg ccttccatgc gctcgtcggt ggccacgcgc tgccagtcga
     7741 acaggaagcg catgaaatcg gcggcggcga ctggctcgac ctctcggcgc agccgcttca
     7801 gcgtatagcg atggatgcga gcgaggagcc ggcgctcgca ccactcgccg ctggcggcgt
     7861 ccaccgtgaa cttgccgcgc atggcgaacc cctcggactc aagcgccgcc agcgcgatct
     7921 ccacgtcggc gaccggcact tcaatatccg cggcaagtgt cgctgcagta acgggcccca
     7981 gcccttcgag gcgcccgcgc acgatttcga ccagcgcggc ttcgcgcgtc cagtctttct
     8041 gcgcgaattc cgccggcgcg ctgatctccg gctcgacacg tgagccggga tgcacggcgt
     8101 ggaattccgg cagccgttcc gccgcgatcc agaagccgtt cgcccccggc gtggcgggga
     8161 tgattctggc agcgcgcttg ctgagagcca gctcttgcag taagccggac caggcgggct
     8221 gtgctctcac ttcttcctgc gtcaacaagc cgagccatac cagggcatcg tgcagctcgt
     8281 ccgcgttatc cgccgccggc cacgcctcct ctttcacgcg cacgatcgcc tcgggatcga
     8341 gccggccgat gtccgccgcg ctctcgggat cgagccagcg gcggctcacc accgcctggg
     8401 tgcggcgctc ttccagcggg gcatcgtcga gaaacgcgta cggccgcgcc gagaggattt
     8461 cctgcgcgag cggcgagggc tcgacgaggt cgcgcgcggt gacctgcact tccccccgct
     8521 ccaggctccg cagcaggcgt tccagcccct cgacgtccat cgcctcgtgc aggcaatcgg
     8581 aaatcgtctg gttcaccagc ggatgatcgg gaatctcgcg gtcgccggcg atgttctcct
     8641 ggcaggcgag ctggtcgggg aagatcgccg cgatcaggtc ctcggcttcc atgcgctgta
     8701 attgcggcgc caccttgttc ccgccccgga aacgcaacag ggcgagcgcg atggaggcat
     8761 tccagcgcca ccgcgtcatg aacatgggcg catcgagcaa cgcctgcgtg agcacttcgc
     8821 acacggtgtc ggcgttgagg tagcgggcga cttcttcgag cgggaagctg tgggtcgagg
     8881 tgagcgacag gatgatcgcg tcctcggtgg ctgccgcctg cagctcgaag ttgaacttgc
     8941 ggcagaagcg cttgcgcagc gcgagccccc aggcgcggtt gacgcggttg ccgtaaggcg
     9001 agtggatcac gagctgcgcg ccgccggtct cgtcgaagaa gcgctcgaac accaggtgac
     9061 cctgggtcgg cagcaggccc agcaccgctt tggccgcggc gaagtagtcc acgatctgct
     9121 gcgcggcttc cgcggcgatg ccgatttcct ccgtgagatg ggccagcgcg gcgctgatgt
     9181 cgccgagccg cgtctccaga tccccgcgca accgcgacac cgcggccgag acttcgtcgg
     9241 tgcgcgccgg cgcctcgccc agccagaacg gaatcgaggg cggcagcccg gccgcgtcct
     9301 ccacgcgcac gcgtcccgct tccacgcgca ggatgcggta cgaggtgttg ccgagctgaa
     9361 agatgtcgcc cgccatgctc tccaccgcga aatcctcgtt taccgtgccg atcacctgct
     9421 cttccggggc cagcaccacg gcgtagtccg ccgtgtcggg tatcgttccg ccgcaggtaa
     9481 cggcggtgag ccgcaatccc tggcgcgggc ggagcttccg gttgaccgcg tcgcgatgta
     9541 cgacggcgcg gctgcggccg cggcgggtgg cgtagccttc ggagagcatc tgcaccaccg
     9601 cgccgtattc ttcacgctcg agtccgcgat acggccaggc gtggcgcagc aggtcgaaca
     9661 gcgcatcctc atcccactca ccacacgcga cttcggccgc aatctgctgc gccagcacgt
     9721 cgagcggctt ctgcgggatg cgcagccgat cgagttcgcc gcgccgcacc gcgtcgagca
     9781 gcgcggcgca ctcgacgaga tcgtcgcgcg acagcgggaa cagacgccct ttcggcaatc
     9841 caccaaccgc gtgaccggaa cggcccaccc gttgcaacag cgccgagatc gcgcgcggcg
     9901 tgccgagctg gcataccagg tccacgtggc cgatgtcgat gccgagctcg agcgatgaag
     9961 tcgccaccag cgccttcagc tcgccgcgct tcaaccgctg ctcggcgttc agccgctgct
    10021 cgcgcgcgag gctgccgtgg tgcgaagtca cctcgccctc gcccagccgc tcggtgagcg
    10081 cgcgcgccgc gcgctcggcg agcctgcggg tgttgacgaa caccagcgtg gagcggtgct
    10141 cgcgcacgag ttccgcgagc cggtcgtaga cctgttccca tacttcggcc gacatcaccg
    10201 cctccagcgg cgattcgggc gcttcgagcg cgagatcgcg cttgcgcgcg tggccggtat
    10261 caacgatggt gcagccggaa cccgcagtcc gatcgtcgaa caacccggcg gcaggacggt
    10321 tcctgccgcc ggtcaggaag cttgccacct cttcgatcgg cttctgcgtg gcggacagcc
    10381 cgacccgggt gagcttcgcc ccggcaacgt gctccagccg ctccagcgac agcgccaggt
    10441 gcgcgccgcg cttgttgggc gcgagcgcgt ggatctcgtc cacgatcacg gtgcgcgtgg
    10501 tggcgagcgc tcttcgaccc gattcgctgg taaggaggat gtagagcgac tcgggcgtcg
    10561 tgacgaggat gtgtggcggc ttgcgccgca tgcgctcgcg ctccgcctgc ggcgtgtcgc
    10621 cggtcctgac cagcgtgcgg atctcgacgt cgggatgacc gagcgcggcg agctccttgc
    10681 ggattcccgc gagcggctct tcgaggttct tgtggatgtc gttggagagc gccttgagcg
    10741 gcgagacgta caccacacgg gtctcgtccc c
//
LOCUS       DBNGWZ010000134         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2279997, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000134 DBNGWZ010000000
VERSION     DBNGWZ010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2279997"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1137)
                     /locus_tag="AC16CX_04000"
     CDS             complement(169..1137)
                     /locus_tag="AC16CX_04000"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011709.1"
                     /translation="MRLTRGIFAGIAAALLAPLVFAAETWPDKPIRVILSVPAGATPD
                     VTARLVTPGLSRLLGQQLVVDNRGGAGGLIGAEIVAKAAPDGYTVFISSPGALTILPH
                     LRKVPYDTLKDFTPISLISIGPFVLITHPSVPARTVKELIALAKAQPGKLNYASAGNG
                     VANHLAMELFKQMAGVDITHVPYKGAPQAVTDVLAGHMNMMFNSIAPIVAHIKAGRVR
                     VLGIASAKRSAQLPDVPTISEAGVPGFEAVNWFGMFAPAKTPKRIVTRLNEAVVKVVR
                     APETRAQFAALGADAVGSSPEEFAAFVRRDMEQYAKVVRISGAKLD"
     gene            1319..2074
                     /locus_tag="AC16CX_04005"
     CDS             1319..2074
                     /locus_tag="AC16CX_04005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3011710.1"
                     /translation="MATIHAAELTGKVALVTGASRNIGRAIALALGAGGAAVAVNART
                     SKEDAEKVAQEIRGAGGQAEAFMADIVDAAAVNAMVEGTVGRFGRLDFLVLNASVRKE
                     TPFAEMSFEEWKNLTAVTLDGSFHCCKACLPHMIKAGGGAIVTIGGMSALSGAKRRVH
                     GSVGKYGLYGMTRALAKELGEHHIRVNCVAPGQMNTARAADRSARPDVSNVPLGRRVE
                     PEEIAGMVRFLCGPGAAMVSGQLIYVDGGQQMF"
BASE COUNT          367 a          711 c          714 g          355 t
ORIGIN      
        1 ggcgggctgt gaaccacgat cctcaccgcc cgggcgggat actgctgcgc cagcgcgggc
       61 tcgcccgccg ctgcgcaaag gagaaaggcg aaagtcgaaa ggagaaagta aagcaagcgt
      121 ttcagcatcg ttctttcgcc tttcgtcttt cgcctttcgc cttgctcgtc agtccagctt
      181 ggcgccggaa atccggacca ccttcgcata ctgctccatg tcccggcgga caaatgccgc
      241 gaactcctcg gggctgctgc cgacggcgtc ggcgcccagc gccgcgaact gggcccgggt
      301 ttccggcgcg cgcacgacct tcaccaccgc ctcgttcagg cgggtgacga tccgcttcgg
      361 ggtcttcgcg ggggcaaaca tcccgaacca gttcaccgcc tcgaaccccg gcacccccgc
      421 ttcgctgatc gtcggcacgt cgggcaactg ggccgagcgc ttcgcgctgg cgatgccgag
      481 cacccgcacc cgcccggcct tgatgtgcgc gacgatcggc gcgatcgagt tgaacatcat
      541 gttcatgtgc cccgccagca cgtcggtcac cgcctgcggc gcgcccttgt acggcacgtg
      601 cgtgatgtcg acgccggcca tctgcttgaa cagctccatc gccaggtggt tggcgacgcc
      661 gttgccggcc gaggcgtagt tgagcttgcc gggctgtgcc ttcgcgagcg cgatcaactc
      721 cttcaccgtc ctcgccggga ccgacgggtg ggtgatcagg acgaaaggcc cgatcgagat
      781 caggctgatc ggcgtgaaat ctttcagcgt atcgtaggga accttccgca ggtgcggcag
      841 gatcgtcagc gctccgggac tgctgatgaa caccgtgtaa ccgtcggggg ccgccttggc
      901 cacgatctcc gccccgatca gcccgcccgc gccgccgcgg ttgtccacga cgagttgctg
      961 gccgagcagc cgcgacagac cgggcgtcac caggcgcgcg gtgacgtccg gcgtcgcgcc
     1021 ggcgggcacc gaaaggatca cccggatcgg cttgtcgggc caggtttctg cggcgaatac
     1081 aagcggcgcc agcagcgcag ccgcgattcc cgcgaagatt ccgcgcgtga gcctcacaaa
     1141 gatctccacc ctcagatcag tgaatggtaa acggtaaacg gcgaatggtg aagaggagtt
     1201 ccagtcacga atcaccagtc acgagtcact cccgcaagct cggccgccca aacgggcgac
     1261 ggtttccgcg caaatcggct atccttggaa atgagactct tcaaggggag acatctttgt
     1321 ggcaacaatt catgcggccg agctaacggg aaaggtggcg ctggtcaccg gcgcctcgcg
     1381 caacatcggg cgcgccatcg cgctcgcgct cggggcgggc ggcgccgcgg tcgcggtcaa
     1441 tgcccgcacg tcgaaggagg acgcggagaa ggtcgcgcag gaaatccgcg gggcgggcgg
     1501 ccaggcggaa gcgttcatgg ccgacatcgt ggatgccgcc gcggtgaacg cgatggtcga
     1561 aggcacggtc gggcgcttcg gccgcctcga ctttctcgtg ctcaacgcct cggtgcgcaa
     1621 ggaaacgcct ttcgccgaga tgagcttcga ggagtggaag aacctcaccg cggtcaccct
     1681 cgacggctcg tttcactgct gcaaggcctg cctgccgcac atgatcaagg ccggcggcgg
     1741 cgccatcgtc acgatcggcg gcatgtccgc gctctccggc gccaagcggc gcgtgcacgg
     1801 ctcggtcggg aagtacgggc tctacggcat gacgcgggcg ctcgcgaagg agctggggga
     1861 gcaccacatt cgcgtcaact gcgtcgcccc cggccagatg aacaccgcgc gcgcggccga
     1921 tcgctcggcc cgtccggatg tatccaacgt gccgctcggg cgccgcgtcg agccggagga
     1981 aatcgccggc atggtgcgct ttctgtgcgg ccccggcgct gccatggtga gcgggcagct
     2041 gatctacgtc gacggcggcc agcagatgtt ttagccgaaa ggcgtaacga gcgagagcta
     2101 atctaagttg gttctcggat aggggctttt atgcgtattc tgcacct
//
LOCUS       DBNGWZ010000135         4262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1650983, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000135 DBNGWZ010000000
VERSION     DBNGWZ010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1650983"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /gene="glgC"
                     /locus_tag="AC16CX_04010"
     CDS             <1..1128
                     /gene="glgC"
                     /locus_tag="AC16CX_04010"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR3011711.1"
                     /translation="DFTLSNCVNSGVRRIGVVTQYKAHSLIRHIQRGWSFLDGRFDEF
                     VQLLPAQQQVEESWYRGTADAVFQNLAVLRRHAPDHVLVLAGDHVYKMDYGKMLAFHC
                     ERGADMTVGCVEVPLQDAGGLGVMAVDNDLRVLGFEEKPASPKAMPGNPGRALGSMGV
                     YAFNADFLIEQVLRDADDPRSTHDFGKDVIPHIVSRYRVFAHRFADSCVGMQPDGEPY
                     WRDVGTVDAYWEANIELTKVVPELNLYDRDWPIWTYQEQLPPAKFVFDDEGRRGMAVD
                     TMVSGGCIVSGASVRRSLLFSGVRVDSYSRVEDTVVLPHARIGERAVLKRVVVDRGCN
                     IPDGTVIGADPEEDGRRFFVTERGITLVTPTMLGQPVHGVR"
     gene            1357..3054
                     /locus_tag="AC16CX_04015"
     CDS             1357..3054
                     /locus_tag="AC16CX_04015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR3011712.1"
                     /translation="MSAGGVDLILLWHMHQPDYRDRARGEFELPWVYLHAIKDYTDMA
                     AHLERHPRVRAVVNFVPVLLDQIEDYVGQIERGELRDPLLRLLAVPDLDALDTAQRRF
                     LLESCSHSNHAQMVVPFSHYKRLFDLRRLVDEAGDAGRRYLSGAYLADLVTWYHLAWT
                     GETERRSRPLLAELMAKGEGYTHADRMRLLKLIGELMKDLVPRYRALAEAGRIELSTT
                     FHSHPIAPLLIDFAAARESQFDAVLPLAPRYPGGRQRVAVQLERAVASHARRFGSPPA
                     GLWPSEGAVSDETLAIMARHGVSWTASSESVLANSLQREGKPGVPRERFVCRSYQLEA
                     QPGVSIFFRDEHLSDLIGFEYAKWHGSDAARHFIAQLEAIGKAAGGGERPLVCVMLDG
                     ENAWENYPYNAYYFFEELYGALEAHPAIRTTTPSSWLREHPGHGGVLRSLVAGSWVYG
                     TFSTWIGAPDKNRAWDLLCSAKQSYDLVVASGRLDAGRRELADAQLMACEASDWFWWL
                     GDYNPEQSVASFDRLFRLNLGNLYATLGLTPPDEVGEPLSRGTGAPEAGGAMRRARG"
     gene            3066..>4262
                     /locus_tag="AC16CX_04020"
     CDS             3066..>4262
                     /locus_tag="AC16CX_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR3011713.1"
                     /translation="MALALLFGVHGHQPVGNFPAVLDETNARCYRPFLETLHRYPAFR
                     FSVHFSGWLLEYLGQRFPREIALLREMVERGQVEVFGGGDYEPVLAAIPHRDRIGQLN
                     AMAARLKRHFGVEPRGAWLTERVWEASVVTALAECGIRYVTVDDYHFACTGMPLEALD
                     GYYTTEEDARTLDVFPISEQLRYRFPFSPAEDAVAYLQAQARAGRTAAIYFDDIEKFG
                     IWPETWDWVYGQRWLERFIEGVLAAPDVRTATYREFHEAHATRGIVYLPTTSYVEMNE
                     WTLPPVAAEAYADLTRREKEAERYERDKGFIRGGIWRNFLSRYPEANWMHKRMLALSR
                     RLAEAASGGGGSALAESLYRAQGNDAYWHGLFGGIYLPHLRRAVYRELLEVERGLDAL
                     SPRPPAA"
BASE COUNT          675 a         1410 c         1449 g          728 t
ORIGIN      
        1 gatttcacgc tttcgaactg cgtcaattcc ggcgtgcgcc gcatcggcgt cgtcacccag
       61 tacaaggcgc acagcctgat ccgccatatc cagcgcggct ggagcttcct cgacgggcgc
      121 ttcgacgagt tcgtccagtt gcttcccgcg cagcagcagg tggaggagtc ctggtaccgc
      181 ggcaccgccg atgcggtgtt ccagaacctc gccgtgctgc gccggcacgc gccggatcac
      241 gtcctggtgc tcgcgggcga ccacgtgtac aagatggatt atggcaagat gctcgcattt
      301 cactgcgagc ggggggcgga catgacggtc ggatgcgtcg aggtcccgct gcaagacgcg
      361 ggcggactcg gcgtgatggc ggtcgacaac gatttgcggg tcttgggctt cgaagagaag
      421 ccggcgagcc ccaaggccat gcccggcaac ccggggcggg cgctcggcag catgggcgtc
      481 tacgcattca acgcggattt cctgatcgag caggtcctgc gcgacgctga cgatccgaga
      541 tcgacgcacg atttcggcaa ggacgtgata ccgcacatcg tgtcgcgcta ccgcgtgttt
      601 gcgcaccgct tcgccgacag ctgcgtcggg atgcagccgg acggggagcc gtactggcgc
      661 gacgtcggca cggtggacgc gtactgggaa gccaacatag agctcaccaa ggtggtcccc
      721 gagctcaatc tttacgaccg ggactggccg atctggacct atcaggaaca gctgccccct
      781 gcgaaattcg tgttcgacga cgaagggcgc cgcggcatgg cggtggatac gatggtgtcc
      841 ggcggctgca tcgtgagcgg cgccagcgtc cggcgttcgc tgctgttctc gggcgtccgg
      901 gtggacagct acagccgggt cgaggacaca gtggtcctgc cgcacgcgcg catcggcgag
      961 cgcgccgtgc tgaagcgcgt ggtggtggac cgcggctgca acatccccga cgggaccgtc
     1021 atcggcgccg atccggagga ggacgggcgg cggttcttcg tgaccgagcg cggcatcacg
     1081 ctggtgacgc cgaccatgct cgggcagccg gtccacggcg tgcgctagtg tcatatcaac
     1141 ccttgaacga aaggataaga acaaaaccag aacggacagg attaacagga ttaacgagat
     1201 ccaaactgca cacctcgaac cgcacctgat tttgttcttg ggctgatcct gtaaatcttg
     1261 taaatcctgt ctattttgtc tttgggtgcg gtctttattc cgtatgtatc tgtcgtcatc
     1321 tgcggtttca tttgatttga gacaacgtcc agccacatga gcgccggcgg cgtcgatctc
     1381 atcctgctct ggcacatgca ccagcccgac taccgcgacc gcgcgcgcgg ggaattcgag
     1441 ctgccgtggg tctacctgca cgcgatcaag gattacaccg acatggccgc gcacctcgag
     1501 cgccacccgc gggtgcgcgc cgtggtgaat ttcgtgccgg tgctgctcga ccagatcgag
     1561 gactacgtcg gccagatcga gcgcggcgag ctgcgcgatc cgttgctgcg cctgctcgcg
     1621 gtgcccgacc tcgatgcgct cgacaccgcg cagcgccggt tcctgcttga aagctgcagc
     1681 cactccaatc acgcgcagat ggtcgtgccc ttctcccatt acaagcgatt gttcgatctg
     1741 cgccgcctcg tggacgaggc gggagacgcg gggcgccgct acctttcggg cgcgtacctc
     1801 gccgacctcg ttacctggta tcacctggcg tggaccgggg aaaccgaacg ccgcagccgt
     1861 ccgctgctcg cggagctgat ggcgaagggc gaaggctata cgcatgcaga ccggatgcgc
     1921 ctgctcaagc tgatcggcga gctcatgaag gacctggtgc cgcgctaccg cgcgctcgcg
     1981 gaggcggggc gcatcgagct ctccaccacg ttccacagcc atccgatcgc tccgctgctg
     2041 atcgacttcg cggccgcgcg cgaaagccag ttcgatgccg tgctgccgct cgcgccgcgc
     2101 tatcccggtg gccgccagcg ggtcgcggtg cagctcgagc gtgcggttgc gagccatgcc
     2161 cggcgcttcg ggtcgcctcc cgccggcctg tggccctccg agggcgccgt atcggacgag
     2221 acgctcgcca tcatggcgcg gcacggcgtg agctggaccg cttcgagcga gagcgtgctc
     2281 gcgaacagcc tgcagcgcga gggtaagccc ggcgtgcccc gggagcgctt tgtctgccga
     2341 tcgtaccagc tcgaggcgca gcccggcgtt tcgatcttct tccgcgacga gcacctgtcc
     2401 gacctgatcg ggttcgagta tgcgaagtgg cacggcagcg atgccgcgcg gcatttcatc
     2461 gcccagctcg aggcgatcgg caaggcggca ggcgggggcg agcggccgct cgtgtgcgtg
     2521 atgctcgacg gcgagaacgc ctgggagaac tacccgtaca atgcctatta ctttttcgag
     2581 gagctctacg gagcgctcga ggcgcacccg gcgatacgga ccacgacacc gtcatcctgg
     2641 ttgcgcgagc accccgggca cggtggcgtg ctacggtcgc tggtcgcggg aagctgggtc
     2701 tacggcacgt tctccacctg gatcggggcg ccggacaaga accgcgcctg ggacctgttg
     2761 tgctcggcca agcagagcta cgacctggtc gtggcgagcg ggcggctcga cgccggccgg
     2821 cgcgaactcg cggacgccca gctcatggcc tgcgaggcct cggactggtt ctggtggctt
     2881 ggcgactata accccgagca gtccgtggcg agcttcgacc ggctgttccg tctcaatctc
     2941 gggaacctct acgccacgct cgggctcacg ccccccgatg aagtcggcga gccgctgagc
     3001 cgcggcaccg gcgcgccgga agccggcggc gccatgcggc gcgcgcgcgg ctgaggcagc
     3061 gcggcatggc gcttgcgctg ctgttcggcg tgcacggcca tcagccggtg ggcaacttcc
     3121 cggcggtgct cgacgagacg aacgcccgct gctaccgccc tttcctcgag accttgcacc
     3181 gctacccggc gtttcgcttc agcgttcatt tttccggctg gctgctcgag tacctgggcc
     3241 agcggtttcc gcgcgagatc gcgctgctgc gcgagatggt cgaacgcgga caggtggaag
     3301 tcttcggcgg gggcgattac gagccggtgc tggccgcgat tccccaccgt gaccgcatcg
     3361 ggcagctgaa cgcgatggcg gcgcggctga agcggcattt cggcgtggag ccgcggggcg
     3421 cctggctcac ggagcgcgtg tgggaagcga gcgtcgtcac cgcgcttgcc gagtgcggca
     3481 tccgctacgt gacggtggac gattaccact ttgcctgcac cgggatgccg ctggaggcgc
     3541 tcgacggcta ctacaccacc gaggaggacg cgcgcacgct cgacgtgttc ccgatttccg
     3601 agcagctgcg ctatcgcttt ccattctcgc cggccgagga cgcggtcgcc tacctgcaag
     3661 cgcaggcccg agcggggcgg accgcggcga tttacttcga cgacatcgag aaattcggga
     3721 tctggcccga gacctgggac tgggtctacg ggcagcgctg gctcgagcgg ttcatcgagg
     3781 gcgtgctcgc cgcgccggac gtgcgcaccg ccacttaccg cgagttccac gaagcacacg
     3841 cgacgcgcgg catcgtgtac ttgcccacga cctcgtacgt ggagatgaac gagtggacgc
     3901 ttccgcccgt ggcggcggag gcgtacgctg acttgacccg ccgggagaag gaagccgaac
     3961 gctacgaacg cgacaagggc ttcatccgcg gcggaatctg gcgcaacttc ctctcgcgct
     4021 atcccgaggc gaactggatg cacaagcgca tgctcgcgct gtcgcggcgc cttgccgagg
     4081 ccgcgtccgg cggcggcggt tccgcgctcg ccgagtccct ctaccgcgcg cagggcaacg
     4141 acgcttactg gcacggcctg ttcggtggaa tctacctgcc gcacctgcgg cgcgccgtgt
     4201 accgggaact gctcgaggtc gagcgcgggc tcgacgccct ctcgccgcgg cccccggccg
     4261 cc
//
LOCUS       DBNGWZ010000136         6493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2783872, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000136 DBNGWZ010000000
VERSION     DBNGWZ010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2783872"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..419
                     /gene="secG"
                     /locus_tag="AC16CX_04025"
     CDS             33..419
                     /gene="secG"
                     /locus_tag="AC16CX_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR3011714.1"
                     /translation="MEILVLVIHVLAALALIGLVLLQHGKGADVGAAFGSGASGSLFG
                     ASGSANFLSRSTAIAAMVFFLTSLGLTYFSANKSEHKGLMATPPAQEKSLPAQIPSST
                     PEGQAAPVGQTAPAAEPGSKVQEVPK"
     gene            514..598
                     /locus_tag="AC16CX_04030"
     tRNA            514..598
                     /locus_tag="AC16CX_04030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:548..550,aa:Leu,seq:gag)
     gene            726..1094
                     /locus_tag="AC16CX_04035"
     CDS             726..1094
                     /locus_tag="AC16CX_04035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3011715.1"
                     /translation="MLENYFPILMFIFVGAAVGVAPVIAGSVVSRLLGVHRPDAEKLS
                     PYECGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVLQEIGFFGFMSMMVF
                     LGILVVGFIYEWKKGALEWE"
     gene            1121..1600
                     /locus_tag="AC16CX_04040"
     CDS             1121..1600
                     /locus_tag="AC16CX_04040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR3011716.1"
                     /translation="MGAPESFHDRGFITTTADALINWTRTGSLWPMTFGLACCAVEMM
                     HAGAARYDLDRFGIVFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGS
                     CANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGIIQLQNKIRRTNTIAR"
     gene            1613..2224
                     /locus_tag="AC16CX_04045"
     CDS             1613..2224
                     /locus_tag="AC16CX_04045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3011717.1"
                     /translation="MTTRAETVIAALQSTLGDKLAGASTALGEATIVVKPEQLLAVAA
                     ALRDAPALKFEQLTDLCGVDYRDYGDGGWEGRRFAVVYHLLSITHNRRVRMRVFAADD
                     AFPVVDSVIGIWPSANWYEREAFDLYGIVFTGHPDLRRILTDYGFVGHPFRKDFPLSG
                     NVEMRYDPDQQRVIYQPVTIEPREIVPRIVRVENYADTEVKKG"
     gene            2312..3565
                     /locus_tag="AC16CX_04050"
     CDS             2312..3565
                     /locus_tag="AC16CX_04050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR3011718.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIERADPHIGLLH
                     RATEKLAEHRTFIQSVPYMDRLDYVSMMVNEHAYVMAIEKLLGIEVPVRAQYIRVMFD
                     EITRLLNHLLWLGTHGLDIGAMTVFLYCFREREDLFDTYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPQYTASRIHNEAAVRELNNNRQGSLLDFIEDFTDRFPQYVDEYETLLTDNR
                     IWKQRTVGIGVVSPERAIALGFTGPMLRGSGVEWDLRKKRPYEVYDRIEFDVPVGVNG
                     DCYDRYLVRIEEMKQSNRIIRQCIEWLRKNPGPVMVDNHKVTPPSRVAMKTNMEELIH
                     HFKLFTEGMHVPAGEAYAAVEHPKGEFGIYLVSDGANKPYRLKIRAPDFAHLSALDEM
                     VRGHMIADVVAMIGTIDLVFGDIDR"
     gene            3562..4050
                     /gene="nuoE"
                     /locus_tag="AC16CX_04055"
     CDS             3562..4050
                     /gene="nuoE"
                     /locus_tag="AC16CX_04055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR3011719.1"
                     /translation="MNAPMVLSPEALARIDRAIAKYPPDQRQSAVMAALTVAQDEKGW
                     LSTETMDFVAQYLGMPPIAVYEVASFYTLYDLKPAGKYKLAICTNLPCALQGANAAAE
                     HLKNKLGIGFDETTADGLFTLKEGECFGACGDAPVLLLNNKRMCCAMTPDKLDDLLKE
                     LK"
     gene            4107..5363
                     /gene="nuoF"
                     /locus_tag="AC16CX_04060"
     CDS             4107..5363
                     /gene="nuoF"
                     /locus_tag="AC16CX_04060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3011720.1"
                     /translation="MKSLDGLNWRLKDYEARGGYAALKKILAEKTPPEQIVAEVKKSA
                     LRGRGGAGFPTGLKWSFMPRQFPGAKYLVCNSDEGEPGTFKDRDILRYNPHIVIEGVI
                     IAGYATGAAVGYNYVHGEIWEVYERFEEALAEARAAGYLGKNILGSDFSFELHAHHGW
                     GAYICGEETGLLESLEGKKGMPRFKPPFPASYGLYGKPTTINNTETFAAVPWIILNGG
                     GAFLNLGRPNNGGTKIFSVSGDIERPGNFEARLGAPFAKLLEMAGGMRGGRKLKACIP
                     GGSSMPVLPAAVMMKTDMDYDSIAKAGSMLGSGAVIIMDETRCMVRCLLRLSYFYFEE
                     SCGQCTPCREGTGWLYRMVHRIEHGKGRLEDLDLLNNVSDNIAGRTICALGDAAALPV
                     KSFIKHFRDEFVYHIEHKKCLVPDYL"
     gene            5439..>6493
                     /gene="nuoG"
                     /locus_tag="AC16CX_04065"
     CDS             5439..>6493
                     /gene="nuoG"
                     /locus_tag="AC16CX_04065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR3011721.1"
                     /translation="MLEIEIDGKQMEVADGATVMDAANQAGIYIPHFCYHRKLSIAAN
                     CRMCLVQVEKAPKPLPACATPVTNGMKVYTHSAQAVDAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGASGSRYEEPKRVVADKNLGPLISTDMTRCIHCTRCVRFGQEIA
                     GIMELGMIGRGEHAEIITFVGKTVDSELSGNVIDLCPVGALTSKPFRYTARTWELTRK
                     PSVSPHCGLGSNLTVQVKQNRVMRVLPRENDAINECWLSDKDRFSYEGLNSEERLAKP
                     MLRRGGTWREVEWQVALDFVAKELRRIRDQHGASSIGALATPHQTLEELFLLGKLARE
                     FASGNVDFRLRQSDFSA"
BASE COUNT         1228 a         2033 c         2069 g         1163 t
ORIGIN      
        1 ccgaggaagt cgcgaggata taggaacgct aaatggaaat tctggttctg gtgatacatg
       61 tactggcggc gctggccctc atcggcctcg tgctgctgca gcacggcaag ggggccgacg
      121 tcggcgctgc tttcggcagt ggcgcttccg gcagcctgtt cggggcctcg ggatcggcca
      181 acttcctgag ccgatcgacg gcgatcgcag cgatggtgtt tttcctgacg agcctcggct
      241 tgacctactt ctcggcgaac aagtccgagc acaagggctt gatggcgacg ccgccggccc
      301 aggagaaatc cctgcctgcg cagattcctt cgagcacgcc ggagggacag gcggcgcccg
      361 tcggtcagac tgcgccggcc gcggaaccgg gctcgaaagt gcaggaagtc ccgaaatgaa
      421 ttcggaatga tgagtggcga atgacagatg atgaatggca ccgatcgcgc cacgggattt
      481 attcacgcgg cattcatcat tccgaattca tgagccgacg tggtggaatt ggcagacaca
      541 ccatcttgag ggggtggcgg cgcaagccgt gtgagttcga atctcaccgt cggcaccagt
      601 tgttaacgta ggagtggggt tcgggaatca ggattcggcg ctcgcagccg tggtcttgaa
      661 ccgagccgat gccgaaaaag gcaggttgac ccggatatag cacatttgat tcgcgccgaa
      721 tcctgatgct ggaaaactac tttccaatcc tgatgttcat atttgtcggc gccgcggtcg
      781 gcgttgctcc cgtgatcgcg ggcagtgtcg tcagccggct gctcggcgtg caccgtcccg
      841 acgccgaaaa gctctcgccc tacgaatgcg gcttcgaggc gttcgaggac gcgcgcatga
      901 agttcgacgt gcgttactac ctggtggcga tcctgttcat cctgttcgac ctcgagatcg
      961 cgtttctctt tccgtgggcg gtggtgctgc aggaaatcgg cttcttcggc ttcatgtcga
     1021 tgatggtatt tctcggcatc ctggtggtcg gcttcatcta cgagtggaaa aagggcgctt
     1081 tggaatggga atgaatttct ccagttagga gatcgtcgtt atgggtgctc ctgaaagctt
     1141 tcacgaccgg ggcttcatca ccacgaccgc cgatgcgctg atcaactgga cgcgcaccgg
     1201 gtcgctctgg ccgatgacct tcgggctcgc gtgctgcgcg gtggaaatga tgcacgccgg
     1261 cgccgcgcgc tacgacctcg accgcttcgg catcgtgttc cggccgagcc cgcgccagtc
     1321 cgacgtgatg atcgtcgccg gcacgctctg caacaagatg gcgcccgccc tgcgcaaggt
     1381 ctacgaccag atggccgagc cgcgctgggt gatctcgatg ggctcctgcg ccaacggcgg
     1441 cggctactac cactattcct attcggtcgt gcgcggctgc gaccgcatcg tgccggtgga
     1501 catctacgtg cccggctgcc cgccgaccgc ggaagcgctg ctctacggca tcatccagct
     1561 gcagaacaag atccgacgca cgaataccat cgcccggtag acagcgcgcc caatgaccac
     1621 ccgcgcagaa accgtcatcg ccgcgctgca atccacgctc ggcgacaagc tggctggcgc
     1681 ctccacggcg ctgggagaag ccaccatcgt ggtgaagccg gagcaactgc tcgccgtggc
     1741 ggccgccttg cgcgatgcgc cagcactcaa gttcgagcag ctcactgacc tctgcggcgt
     1801 ggattaccgc gactacggtg acggagggtg ggagggcagg cgcttcgccg tggtctacca
     1861 cctcctgagc atcactcaca accggcgcgt gcggatgcgc gtgttcgcag ccgacgacgc
     1921 cttcccggtg gtggattccg tcatcggcat ctggccgtcg gccaactggt atgagcgcga
     1981 ggcgttcgac ctctacggca tcgtcttcac cgggcacccg gatctccgcc gcatcctcac
     2041 cgactatgga tttgtcgggc accccttccg caaggatttc ccgctttcgg gcaacgtcga
     2101 gatgcgctac gaccccgacc agcagcgcgt gatctaccag ccggtcacca tcgagccgcg
     2161 cgagatcgtc ccgcgcatcg tccgtgtcga aaactacgcg gacaccgagg tgaagaaagg
     2221 ctgaatcgac aggattaaca ggatttacgt gattagagcc gcgttcttct cgtttgaatc
     2281 ttgttaatcc ggtaaatcct gtccatcaga catggcagag atcagaaact atacgatgaa
     2341 tttcgggcct caacacccgg cggcccacgg ggtgctgcgc ctcgtgctcg agctcgacgg
     2401 cgaggtgatc gagcgcgccg atccgcacat cgggctgctg caccgcgcga ccgagaagct
     2461 cgccgagcac aggaccttca tccagtcggt gccgtacatg gaccggctcg attacgtgtc
     2521 gatgatggtg aacgagcacg cctacgtgat ggcgatcgag aagctgctcg gcatcgaggt
     2581 gccggtgcgc gcccagtaca ttcgcgtcat gttcgacgag atcacgcgcc tgctcaatca
     2641 cctgctctgg ctcggcacgc acgggctcga catcggtgcg atgacggtgt tcctctactg
     2701 cttccgcgag cgcgaggatc tgttcgacac gtacgaggcg gtctccggcg cgcgcatgca
     2761 cgccgcttat taccgtccgg gcggggtcta ccgcgatttg cccgatcgca tgccgcagta
     2821 caccgcgtcc aggattcaca acgaagcggc ggtacgcgag ctcaacaaca accggcaggg
     2881 gtctttgctc gacttcatcg aggatttcac cgaccgcttt ccgcaatacg tggacgagta
     2941 tgagacgctg cttaccgaca accgtatctg gaagcagcgc actgtgggca tcggcgtcgt
     3001 ctcgcccgag cgcgcgatcg ccctcgggtt caccgggccg atgctgcgcg gatcgggcgt
     3061 cgagtgggac ctcaggaaaa agcggccgta cgaggtgtac gaccggattg agttcgacgt
     3121 gccggtcggc gtgaacggcg actgctacga ccgctacctg gtgcgcatcg aagagatgaa
     3181 gcagtcgaac cgcatcatcc ggcagtgcat cgaatggctg cgcaagaacc cgggtccggt
     3241 gatggtcgac aaccacaagg tgacgccgcc ttcgcgcgtg gcgatgaaga ccaacatgga
     3301 ggagctgatc caccacttca agctcttcac cgagggcatg cacgttccgg caggcgaggc
     3361 ttacgccgcg gtcgaacatc ctaaaggaga attcggcatt tacctcgttt ctgacggcgc
     3421 caacaagccg taccgactca agatccgtgc gccggacttc gcgcatctgt cggcgctcga
     3481 cgagatggtg cgcggccaca tgatcgcgga cgtcgtggca atgatcggca cgatcgatct
     3541 cgtgttcggg gatatcgacc gatgaacgcg ccgatggtct tgagcccgga ggcgctcgcc
     3601 cggatcgacc gtgcgatcgc caagtacccg ccggaccaga gacagtcggc ggtgatggcg
     3661 gcgctcaccg tcgcgcagga cgagaaaggc tggctgtcca ccgaaaccat ggatttcgtc
     3721 gcgcagtacc tggggatgcc gccgatcgcc gtctacgagg tcgcctcgtt ctataccctg
     3781 tacgacctca agcccgcggg aaaatacaag ctcgcgatct gcaccaacct gccgtgcgcg
     3841 ctgcaggggg cgaacgccgc ggccgagcat ctcaagaaca agctcggcat cggctttgat
     3901 gagacgacgg cagacgggct gttcacgctg aaggaaggcg agtgcttcgg cgcgtgcgga
     3961 gacgcgccgg tgctgctgct caacaacaag cgcatgtgct gtgccatgac gccggacaag
     4021 ctcgacgacc tgctgaagga gctcaaatag tgggactgcc ggttcctccc tttgcaaccg
     4081 acctgttcgg ccgcgaggcg atcattatga agagcctcga cggcctcaac tggcgcctca
     4141 aggactatga ggcgcgcggc ggctacgcgg cgctgaagaa gattctcgcc gagaagacgc
     4201 cgcccgagca gatcgtcgcc gaggtcaaga aatccgccct gcgcgggcgc ggcggcgcgg
     4261 gcttcccgac ggggctcaag tggagcttca tgccgcgcca gttcccgggc gcgaagtacc
     4321 tggtgtgcaa ttccgacgag ggcgagccgg ggaccttcaa ggaccgcgac atcctgcgct
     4381 acaacccgca catcgtgatc gagggcgtga tcatcgcggg ctacgcgacc ggagccgcgg
     4441 tcggctacaa ctacgtccac ggcgagatct gggaagtcta cgagcgcttc gaggaggcgc
     4501 tcgcggaggc gcgggcggcg ggctatctgg gcaagaacat cctgggctcg gacttcagct
     4561 tcgagctcca tgcccatcat ggctggggcg cctatatctg cggcgaggag actgggctgc
     4621 tcgaatcgct cgaaggcaag aagggcatgc cgcgcttcaa gccgccgttc ccggcgagct
     4681 acggcctcta cggcaagccc accaccatca acaacaccga aactttcgcc gcggtgccct
     4741 ggatcatcct gaacggcggt ggggcgttcc tcaacctcgg ccgccccaat aacggcggaa
     4801 ccaagatctt ctcggtttcc ggtgacatcg agcgccccgg caatttcgag gcgaggctcg
     4861 gcgccccgtt cgcgaaactg ctcgagatgg cgggcggcat gcggggcggg cgcaagctca
     4921 aggcctgcat ccccggcggc tcctcgatgc cggtgttgcc ggcggcggtg atgatgaaga
     4981 ccgacatgga ctacgactcc atcgccaagg cgggctcgat gctgggttcc ggcgcggtca
     5041 tcatcatgga cgagacgcgc tgcatggtgc ggtgcctgct gcggctttcc tacttctact
     5101 tcgaggaatc ctgcggccaa tgcacgccct gccgcgaagg aaccggctgg ctctaccgga
     5161 tggtgcatcg catcgagcat ggcaaaggca ggctcgaaga cctcgatctc ctcaacaatg
     5221 tgtcggacaa catcgccggg cgcaccatct gcgcgctggg cgacgccgcg gcgctgcctg
     5281 tgaagagctt catcaagcac ttccgcgacg agttcgtcta ccacatcgag cacaagaagt
     5341 gcctggtgcc cgactactta tgagagcgcg atcaaccacc ctcacccccg cccctctccc
     5401 tgagggcgag gggaggttcg tgagtcgcgg agcgactgat gctagaaatt gaaatagacg
     5461 gcaaacagat ggaggtcgcc gacggggcga cggtcatgga tgcggccaac caggccggta
     5521 tctacatccc gcacttctgc tatcacagaa agctctcgat cgcggccaac tgccgcatgt
     5581 gcctggtgca ggtggagaag gcgccgaagc cgttgccggc gtgcgccacc cccgtcacca
     5641 acggcatgaa ggtgtacacg cactcggcgc aggcggtcga cgcccagaag ggcgtcatgg
     5701 agttcctgct catcaaccat ccgctcgact gcccgatctg cgatcagggc ggcgagtgcc
     5761 agctgcagga cctggcggtc ggttacggcg cgagcggctc gcgctacgag gagcccaagc
     5821 gcgtggtggc ggacaagaac ctggggccgc tgatctcgac cgacatgacg cgctgcatcc
     5881 actgcacgcg ctgcgtgcgt ttcggtcagg agatcgccgg catcatggaa ctcgggatga
     5941 tcggccgcgg tgagcacgcc gagatcatca ccttcgtcgg caagaccgtg gactcggagc
     6001 tctcgggcaa cgtgatcgac ctctgcccgg tcggcgcgct cacttctaag ccgtttcgct
     6061 acaccgcgcg cacctgggag ctcacgcgca aaccctcggt gagcccgcac tgcggcctcg
     6121 gctcgaacct taccgtccag gtcaagcaga accgcgtcat gcgcgtgctg ccgcgcgaga
     6181 acgacgcgat caacgagtgc tggctgtcgg acaaggaccg cttctcgtac gaagggctca
     6241 actcggagga acggctcgcg aagcccatgc tcaggcgggg cggtacgtgg agggaagtgg
     6301 agtggcaggt cgcgctcgat ttcgtggcga aggagctgag acgtattcgc gaccaacatg
     6361 gtgcctcgag catcggagcg ttggcgacac cccatcagac gctcgaagaa cttttcctgt
     6421 taggcaaact cgctcgcgag tttgcctcgg gcaacgtgga tttccggctg cgccagtccg
     6481 atttcagcgc cga
//
LOCUS       DBNGWZ010000137        17724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_768241, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000137 DBNGWZ010000000
VERSION     DBNGWZ010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17724
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_768241"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..609
                     /gene="pyrE"
                     /locus_tag="AC16CX_04070"
     CDS             10..609
                     /gene="pyrE"
                     /locus_tag="AC16CX_04070"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR3011722.1"
                     /translation="MRFGEFKTKAGRLSPYFFDAGLFYDGAALKQLAQFYAKAIAATG
                     IGFDMLFGPAYKAIPLVAAVSIALADAGRNVRYSFNRKEAKDHGEGGSVIGAPLSGRV
                     LIVDDVISAGTSVRESVNLIRASNAIPCGVAIALDRMERGSGALSAVQEVRASYNVPV
                     ISIATLDDLMEYLRADPRQERLLAAVGRYREQYGVSAHA"
     gene            602..1294
                     /locus_tag="AC16CX_04075"
     CDS             602..1294
                     /locus_tag="AC16CX_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011723.1"
                     /translation="MLRTTRAFLAAAFVIALAALVAGGAQAQSKGKIICWKDKSGKVV
                     GCGDTVPPEYQGSGTKELDKRGVMRGTTESAEDTAKRALQTKETERQKAEEKKRLEEQ
                     KRQDAALLNTFTDEKEIDLKRDRDLQVVNTQISQLRIAHKNAVDRLQDFKGRIQTAEK
                     DKKPPSEYYKEEVARAEADKAKVEQGIAAKEKEKEDIRKRYADMRERYTFLRGGASPA
                     PTAAAAPAAAKK"
     gene            complement(1415..2890)
                     /gene="gatB"
                     /locus_tag="AC16CX_04080"
     CDS             complement(1415..2890)
                     /gene="gatB"
                     /locus_tag="AC16CX_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3011724.1"
                     /translation="MNWEVVIGLETHAQLNTRSKMFSGAATAFGAAPNTQACAVDIAL
                     PGVLPVVNREAVAKAVRFGLAIGATINRRSIFARKNYFYPDLPKGYQISQYEMPIVQG
                     GAISIFVDDEEKAVRLTRAHLEEDAGKSLHEGFHDFTGIDLNRAGTPLLEIVSEPDMR
                     SAQEAVAYAKALHALVRWIDICDGNMQEGSFRCDANVSVRSAGSDKLGTRCEIKNLNS
                     FRFLERAIEFEARRQIEILEEGGSIVQETRLYDPDRDETRSMRIKEEAHDYRYFPDPD
                     LLPLEITESWIGKIKSDLPELPQARRKRYEQELLLFPGDAAALTASREIALYFEAVLA
                     DLAKIPGIPYGTQAPDLANAKHVANWVINDLGSLLNRDNTPIENSPVSAAQLARLLAR
                     LFDKTVSGKTAKDILNVMWAGEGDPDSVIAKRGLKQISDSGELERICDEVIAANPKQA
                     EDYRAGKDRAFNSLVGQVMKLTKGKANPQQVSEILKRKLAP"
     gene            complement(3026..4483)
                     /gene="gatA"
                     /locus_tag="AC16CX_04085"
     CDS             complement(3026..4483)
                     /gene="gatA"
                     /locus_tag="AC16CX_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3011725.1"
                     /translation="MHNATIKSLSADLAARKLSSVELTRHFLSRIKVLNKKYNCFITL
                     DDEASLKQAKAADGMRAAGAGQPLTGVPIAQKDIFCSRGWLTTCGSRMLSNFVSPYDA
                     HVVERLNTAGAVNLGKTNMDEFAMGSSSETSHYGPVRNPWNVDAVPGGSSGGSAAAVA
                     ARLAPGAIGTDTGGSIRQPAALSGVCGLKPTYGVVSRYGMIAFASSLDQGGPMACTAE
                     DLALLLNVMAGFDARDSTSLEREREDYTRDLGKPLEGTRIGLPKEFFAEGAEAGVVKA
                     VGAAIAEYRRLGCKTVEVDLPNMKLSVPVYYVLAPAEASSNLARYDGVRYGYRAPEYS
                     DLIDLYKKTRAQGFGAEVKRRILIGTYVLSHGYYDAYYIQAQKVRRLIARDFAEAFKR
                     CDVIMGPTSPTTAFKLGEKLDDPVRMYLNDIYTIPANLAGLPGLSIPCGFDGKGLPVG
                     LQIIGSYFAEAKMLGVAHQYQLATDWHMKVPPGIE"
     gene            complement(4569..4856)
                     /gene="gatC"
                     /locus_tag="AC16CX_04090"
     CDS             complement(4569..4856)
                     /gene="gatC"
                     /locus_tag="AC16CX_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR3011726.1"
                     /translation="MALTLDDVKRVAHLARLAIDEAEARAVLSQINDVFRLIAEMQAV
                     DTRGVAPMSHALDIVQRLREDAVTEGDQHALFQSVAPHVEADLYLVPKVME"
     gene            4953..5996
                     /locus_tag="AC16CX_04095"
     CDS             4953..5996
                     /locus_tag="AC16CX_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3011727.1"
                     /translation="MFGFLSSYFNDDIAVDLGTANTLIYVRGKGIVLDEPSVVAIRQD
                     GGPNGKKVIQEVGLAAKQMLGRTPGNITAIRPMKDGVIADFTVTEQMLKHFIKKVHES
                     RVFKPSPRIIICVPCGSTQVERRAIRESAIGAGASNVYLIEEPMAAAIGADLPVGEAT
                     GSMVVDMGGGTTEVGVISLGGLVYKGSVRVGGDKMDEAIINYIRRNYGMLIGETTAEN
                     IKKEIGSAFPGSEVREKEVKGRNLAEGIPRSFTISSNEILEALTEPLNSIVSAVKSAL
                     EQTPPELAADIAEKGMVLTGGCALLRDIDRLLMEETGLPVIIAEDPLTCVVRGCGKAL
                     EKIERLSSIFTND"
     gene            6092..6988
                     /gene="mreC"
                     /locus_tag="AC16CX_04100"
     CDS             6092..6988
                     /gene="mreC"
                     /locus_tag="AC16CX_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3011728.1"
                     /translation="MEHQPPPFFKTGPTPLARLLIFSTLSLAFLVADVRFNYLGTLRQ
                     VAAVIVYPFQRLATTPASIAGRIGEFFVTHGWLREENARLVRQNFENAALLQQLEALQ
                     AENAQLRELLATRSRLDVELVAAEVLYAARDPFTRKVVIDKGSQHEVRPGQPVVDNRG
                     VVGQVTRVYSWLAEVTLVTDKGQFVPVQNLRNGLRAVLAGTGNDGALELRFIPLNADF
                     QNGDRLVTSGIDGIYPPGLPVAQVTHVERSAEQLFARITCAPLGGVANHAHVLIVRSQ
                     RETPPRPVEEPQPSRPGKPRKD"
     gene            6988..7509
                     /gene="mreD"
                     /locus_tag="AC16CX_04105"
     CDS             6988..7509
                     /gene="mreD"
                     /locus_tag="AC16CX_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3011729.1"
                     /translation="MADARITRHVILLPVKVGFIVFSLIAALLLNLLPWSGAALWIKP
                     DFVALVVLYWCIEQPRKVGFVAAWLLGLFMDVADGTLFGQHALAYSILAYAGIVLNRR
                     VRMFSGTPQVLHVVILLLMNDLIVIGIRAAAGADFPGFQYFIGSFVAGALWLPLGVLL
                     KLPQRPRPDPDHV"
     gene            7502..9406
                     /gene="mrdA"
                     /locus_tag="AC16CX_04110"
     CDS             7502..9406
                     /gene="mrdA"
                     /locus_tag="AC16CX_04110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3011730.1"
                     /translation="MSELSINPGIELRNAQLELHHFRVRLAIAAGFMLAMFLLLAGRF
                     LYLQVIKHGELQTLAEANRISIVPVMPNRGIIVDRNGAVLAHNYSAYTLEIQPSKVRN
                     LDALIDELGTVVEITPRDRRRFKRLLEESRSIGSLPIRTRLSDEEIARFAANRYRFPG
                     VEIDARLFRQYPQGEVFSHVVGHIGRINQREVEELESEGHAANYRGTDYIGKTGLEQN
                     YERYLHGTTGVEEVEVDSGGRPVRTLSRTPPVSGNNLVLNLDAGLQEAAYLAFGDRRG
                     ALVAIEPGTGGVLAFVSKPGFDPNLFVDGIDPQNWSELNSSPDRPLVNRALSGTYPPG
                     STFKPYMALAALTYGKRTPQQTINDPGYFMFGGHQFRDSRPGGLGVVDMYKSIVMSSD
                     TYYYMLANDLGIDAISRFMGTLGFGSRTGIDILGEATGVLPSQEWKMRRFKQKWFAGE
                     TISIGIGQGYNAYTPLQLAQAIATIANDGVAFQPHIVNYVEDIRTRGRTAIEPRPLRT
                     LDVKPEHLAVIRQALVGVNKEGTGARAFAGAGYVSAGKTGTAQVIGIKQGERYVESRV
                     QERNRDHALFIAYAPADNPKIALAVIVENTGFGARYAAPVARKVFDYYFLGKLPEPDK
                     RGKDDDESGN"
     gene            9390..10493
                     /gene="rodA"
                     /locus_tag="AC16CX_04115"
     CDS             9390..10493
                     /gene="rodA"
                     /locus_tag="AC16CX_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3011731.1"
                     /translation="MNPAIRRLEYYLSRNVDGVLLGLTVALMIVGLVTLFSASNVNAA
                     RLTAQLANMLVALGVMWLFANIPPHYLMRLAFPLYAIGVLLLVAVALGGDMVHGARRW
                     LDLGITRIQPSELMKIAVPLALAWYFERHEATLRLTNYAVGAVMLLIPVALIARQPDL
                     GTALLITASGFFVLFLAGLSWRIIGGLAVAALAAAPFAWSALHDYQRQRILTLLDPTQ
                     DPLGSGYHTIQSTIAVGSGGWFGKGWMQGTQSHLDFIPERTTDFIFAVYAEEFGLFGS
                     LLLLGLLLLVISRGMVITANAPTLFTRLLAGAITLTFFTYAFVNMGMVSGILPVVGVP
                     LPLVSYGGTALVSICLGLGILMSIQSHKKLVQT"
     gene            10630..11568
                     /locus_tag="AC16CX_04120"
     CDS             10630..11568
                     /locus_tag="AC16CX_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR3011732.1"
                     /translation="MSKRDASAKAPPPPRGGGYYLDDGPGANPPADLDGVPDAVPRLE
                     PLHRGTSRPYVVMGRQYTPMTSLQPYRARGIATWYGRRYHGKQTSSGEIYDMYAMTAA
                     HATLPIPSYARVTNLSNGKSVVVRINDRGPFVDGRIVDLSYTAAHRIGVLGGGSAMVE
                     VEAIIPDGTGPTAVAHAPRPAGSRVAAAAAAPVEAPAAGDAMTASRASEPEAQPLPAA
                     EAQPAAAAATQIPVAAESGGYYLQLGAFGSRENAENFLTRMKAQVEWLASALHIFTRD
                     GLFRVHAGPYPNQSEARQTAERIGQALGIKAMVLVR"
     gene            complement(11594..12007)
                     /locus_tag="AC16CX_04125"
     CDS             complement(11594..12007)
                     /locus_tag="AC16CX_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011733.1"
                     /translation="MTLADEKQGFSKRLKEAMRKARIDISSPTLIAREFNLRYHGDPV
                     STQAVRKWISGKALPSQDKIRALGLWLEVSVHWLRFGEAERKDERQPLAARQESGSYR
                     LDYGWPSRKFELLNDAHKRMVLEMVHALLRLEGKQ"
     gene            12139..13065
                     /locus_tag="AC16CX_04130"
     CDS             12139..13065
                     /locus_tag="AC16CX_04130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3011734.1"
                     /translation="MTWRERDLSKVIEHIPLGVAITLPDGMLEYANPYFCKLIGAFGS
                     ELVGTPLARWLSTDAPPDRGARQSISSGPAWRGETHVRLSSGELLDVLEAVYPLHDKA
                     GILTHLIHLVQDISAQKQLELLSALAFYDGLTGLPNRNLLNDRLTRVIAGARRHYGAF
                     ALLYIDVDHFKEINDTQGHDAGDELLRQLAARLSQSLRAADTVGRWGGDEFVAMLDGV
                     ASPQAATRIIRKLLSICSEPYDLGSGVCSVTLSIGASLFPRDGYDIPTLLKRADEAMY
                     AVKKRGRNGYHLLKEPGSRYRKTVQKEPAMIT"
     gene            complement(13155..13871)
                     /locus_tag="AC16CX_04135"
     CDS             complement(13155..13871)
                     /locus_tag="AC16CX_04135"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3011735.1"
                     /translation="MTAFFGQNNLDARAPAARRNARRLLGAFLAGSVAAHAALLVALP
                     GFSPDREQPKVNALEVVILQPEPLPVALPESTPQPAPLSGAERVPARALPKPQPERTA
                     PILALPEPRATEGSFTVPPDSMPELPVAVPEQRSRVASATMTPPTFNAAYLSNPAPRY
                     PVAARRADEQGTVTLRVLVTREGLPARVDVEKSSGSSHLDAAALEAVKAWRFVPARRG
                     TEAIESWVLVPIVFRLEGAS"
     gene            complement(13939..14445)
                     /locus_tag="AC16CX_04140"
     CDS             complement(13939..14445)
                     /locus_tag="AC16CX_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HIR3011736.1"
                     /translation="MKFAVYVFAMLLVSHAAWAQQKGVPGKDMGPMDAPLPPSPYLDK
                     PLPELKGVVSWKTLAAVTPIKQKDRFVPQFSKDVSALDQKEVKLQGFMMPLDMGEKQK
                     RFLLVALPPSCAFCLPGGPDQLVEVVAKTPVKYGFDPIVVSGKFAVLKDDPMGLYYRM
                     TDAVAVAQ"
     gene            complement(14541..14801)
                     /locus_tag="AC16CX_04145"
     CDS             complement(14541..14801)
                     /locus_tag="AC16CX_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011737.1"
                     /translation="MRALMIVVMTAGLLAVPAAFAAEKGKKDDPAHVKQAVADHLAMA
                     EAHVNAAKCLESGRPEKECHAQLAKDCKGLGIGKYCGMKHRH"
     gene            complement(14852..16045)
                     /locus_tag="AC16CX_04150"
     CDS             complement(14852..16045)
                     /locus_tag="AC16CX_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011738.1"
                     /translation="MNLFKLSFGYLRARALNTALNVALLALGVATITLLLLTTSQLEE
                     RMGRDARGIDLVAGAKGSPMQVILSAIYHLDVPTGNIPLAQAKEIAKHRMIKKTIPLA
                     LGDSYKGFRIVGTNPDYVAHYDGKLAAGRLWQKPMETVIGADVAQRMGHAVGATFTGA
                     HGIAEAGAEHGDAPYQVVGVLAPSGTVLDRLVLTSIESVWLVHDEHMGTDAKVTEEDK
                     EITALLIQYATPIAAAALPRYINQNSEMQAASPAYETARLFSIVAVGVDVLRGFALVL
                     IFSAGLSLFIALYNALAERRYDLALMRTLGASPLRLMMLMLFEGALLAGLGAVLGLAL
                     GHAVTEALGYALKAAQQVAVTGWVWVPGELWLFGLALVVGVVAALLPAWRAYRTDIAH
                     TLARG"
     gene            complement(16045..16680)
                     /locus_tag="AC16CX_04155"
     CDS             complement(16045..16680)
                     /locus_tag="AC16CX_04155"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011739.1"
                     /translation="MFAIQNLRHAYNGTEVLNVPAWQAEQGSHWLVLGPSGSGKTTLL
                     HILAGILKPTAGSVSVAGQDIGALKPEALDRFRGQHVGIVLQRLHLMSSLTVMDNLLL
                     AQYLAGLPQDSARVREVLAGLDLAGKEGAYPHELSFGQAQRVAVARAVVNRPQLLLAD
                     EPTSNLDDGRCMQALELLQTQARACNATLLIATHDRRVKSRMPDQFALGGG"
     gene            16765..17010
                     /gene="yidD"
                     /locus_tag="AC16CX_04160"
     CDS             16765..17010
                     /gene="yidD"
                     /locus_tag="AC16CX_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3011740.1"
                     /translation="MKWLLLRLIGAYQLLLSPFFGQQCRFYPTCSAYAREAIEVHGSL
                     KGSWLAVKRILRCGPWHPGGTDPVPPKGKSRKAKGES"
     gene            complement(17079..>17724)
                     /locus_tag="AC16CX_04165"
     CDS             complement(17079..>17724)
                     /locus_tag="AC16CX_04165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3011741.1"
                     /translation="RIAVTENGLRFEIDLEHGHKTGFYLDQRDNRRRLRELARGRDAL
                     DGFCYTGGFALNAQAGGARSVTAVESSAEALELARANAKLNDLAGVEWIEGNIFQLLR
                     KFRDGGRSFDLVVLDPPKFAPTAAHAAKAARAYKDINLLAFKLLRPAGLLMTFSCSGG
                     VSSDLFQKIIAGAALDAGVDAQIIERLGPGADHPVALNFPEGEYLKGLVCRVAG"
BASE COUNT         3131 a         5905 c         5524 g         3164 t
ORIGIN      
        1 cagaacgtcc tgcgcttcgg cgaattcaag accaaggcgg ggcgcctctc gccgtacttc
       61 ttcgatgccg gtctgttcta cgatggcgcg gcgctgaagc aactggcgca attctacgcg
      121 aaagcgatcg ccgccacggg catcgggttc gacatgctgt tcggcccggc ttacaaggcg
      181 atccctctgg tggcggcggt ctcgatcgcg ctggccgatg ccgggcgcaa cgtgcgctac
      241 agctttaatc gcaaggaggc caaggaccac ggcgaggggg ggtccgtgat cggcgcgccc
      301 ttgtcgggca gggtcctgat tgtggacgat gtgatctcgg ccggcacctc ggtgcgcgag
      361 tctgtcaacc tgatccgggc ctcaaacgct ataccctgcg gggtggcgat cgcgctcgat
      421 cgcatggagc gcggcagcgg ggcgctgtct gcggtgcagg aagtgcgcgc aagctataat
      481 gtcccggtga tcagcatcgc cacgctcgac gatctgatgg agtatctcag ggccgatccg
      541 cggcaagaac ggcttctcgc ggcggtcggc cgctatcgcg aacaatacgg agtttcggcc
      601 catgcttagg acaactcgcg cgtttctcgc cgcggcattt gtgatcgccc tggcggctct
      661 ggttgcaggc ggcgcacagg cccagtccaa aggcaagatc atctgctgga aggacaagtc
      721 gggcaaggtc gtcggctgcg gcgatacggt gccgcccgag tatcagggca gcggcaccaa
      781 ggagctggat aaacgcgggg tgatgcgcgg aaccacggag tccgcggaag atacggccaa
      841 gcgcgcgctc cagacaaagg agaccgagag acagaaggct gaggagaaaa agcggcttga
      901 ggagcagaag cgccaggacg cggcgctgct caacaccttc accgacgaga aggagatcga
      961 tctcaagcgc gaccgtgatc tgcaggtcgt gaacacccag atttcccagt tgcggatagc
     1021 gcacaagaac gcggtcgacc gcctccagga tttcaagggg cggatccaga ccgccgagaa
     1081 agacaagaag cccccttccg aatattacaa ggaagaagtg gcgcgcgccg aggccgacaa
     1141 ggccaaggtc gagcagggca tcgccgccaa ggaaaaggaa aaagaggata tccgcaagcg
     1201 ctacgccgat atgagggagc gctacacctt cctgaggggc ggcgcatcgc cggctccgac
     1261 ggcggcggcg gcgccggcag ccgcgaaaaa atagtttcgc ggccagcatt cagctgtcag
     1321 cgctcggctc tcagccaaat gacaaaagcg gcgccattcg gcgctgcttc ttgttggtgg
     1381 tgtacgctga tggctgattg ctgacggcta ttcgctaagg cgccagcttg cgcttcagta
     1441 tctcgctcac ctgctgggga ttcgcttttc ccttggtgag cttcatcacc tggcccacca
     1501 gggagttgaa ggccctgtcc ttgccggcgc ggtagtcctc ggcctgctta ggatttgcgg
     1561 cgatgacctc gtcgcagatc ctttccagct caccgctatc ggaaatctgt tttagcccgc
     1621 gcttcgcgat gacggaatcc gggtcgccct cgccggccca catcacgttg agtatatctt
     1681 tagcagtctt gccagaaaca gtcttgtcaa aaagacgggc cagcaaacgc gcaagttgag
     1741 cagcggatac cgggctattt tcgatcggag tattgtccct gttcaacaac gaccccaggt
     1801 cattaatcac ccaattggca acatgtttgg catttgccaa atcgggtgct tgtgtgccgt
     1861 aaggtattcc ggggatcttt gcgagatcgg ccaacacagc ctcgaaatac agggctattt
     1921 cgcgcgatgc ggtcagcgct gctgcgtccc cgggaaacag caataattcc tgttcatatc
     1981 gtttacgtct ggcctgcggt agttctggta aatcactttt tattttcccg atccacgatt
     2041 cggttatttc tagcggtaag agatccggat cgggaaaata gcggtagtcg tgcgcctcct
     2101 ccttgatgcg catcgagcgc gtctcgtcgc ggtccgggtc ataaagccgc gtctcctgca
     2161 cgatgctgcc gccctcttcc aggatctcga tctggcgtcg cgcctcgaac tcgatggcgc
     2221 gctcgagaaa gcggaacgag ttgaggttct tgatctcgca gcgggtgccg agcttgtcgg
     2281 accccgccga ccgcaccgag acgttggcgt cgcaacggaa cgagccttcc tgcatgttgc
     2341 cgtcgcagat gtcgatccag cgcacgagcg cgtgcagcgc cttcgcgtac gccacggctt
     2401 cctgcgcgct gcgcatgtcg ggctcagaga cgatctcgag cagcggcgtt ccggcgcggt
     2461 tcaggtctat ccccgtgaaa tcatggaagc cctcgtgcag cgacttgccg gcatcctcct
     2521 cgagatgcgc acgcgtgagc cgcaccgcct tttcctcgtc gtccacgaag atggagattg
     2581 cgccgccctg cacgatcggc atttcgtact ggctgatctg gtagcccttg ggaagatcgg
     2641 gatagaaata gttcttgcgc gcgaagatcg agcggcggtt gatggtggcg ccgatcgcaa
     2701 gcccgaaacg caccgctttc gcgaccgcct cgcggttcac gaccggcagc acgcccggca
     2761 gcgcgatgtc caccgcgcac gcctgcgtgt tcggcgccgc cccgaacgcc gtcgccgcgc
     2821 ccgagaacat cttcgagcgc gtgttgagct gggcgtgggt ctcgagaccg atgaccactt
     2881 cccaattcat cgccgtcgct ccgcgacgag tgacgaggga cgagtgacga gggacgcgtc
     2941 agccgcttcg cccagccctt gcccccgctc atgcgggaga gggcaattat ccactcgaca
     3001 ctcgacactc gacactcttg actcctcact ctatgcccgg aggcaccttc atgtgccaat
     3061 ccgtggccag ttggtactgg tgcgcgacac cgagcatctt cgcctcggcg aagtagctgc
     3121 cgatgatctg cagcccgacc ggcaacccct ttccgtcgaa gccgcacggg atagacaggc
     3181 cgggcagccc cgcgaggttg gcggggatgg tataaatgtc gttgagatac attctcaccg
     3241 gatcgtccag cttctcgccg agcttgaacg cggtggtggg gctggtcggc cccatgatca
     3301 cgtcgcagcg cttgaacgcc tccgcgaaat cgcgcgcgat cagccgccgc accttctgcg
     3361 cctggatgta ataggcgtcg taatagccgt gcgagagcac gtaggtgccg atcaggatgc
     3421 gccgcttcac ctcggcgccg aagccctgcg cccgcgtttt cttgtagagg tcgatcaaat
     3481 cggagtactc cggcgcgcgg tagccgtagc gcacgccgtc gtagcgcgcg aggttgctcg
     3541 aggcctccgc tggcgcgagc acgtaataga ccggcaccga gagcttcatg ttgggcaggt
     3601 ccacttcgac cgtcttgcag ccgagcctgc ggtactccgc aatcgccgct cccaccgcct
     3661 tcaccacacc ggcctccgcg ccttcggcga agaattcctt cggcaagccg atcctggtgc
     3721 cctcgagcgg ctttccgagg tcacgcgtgt agtcctcgcg ttcgcgctcg agcgaagtcg
     3781 aatcgcgcgc atcgaagccg gccatgacgt tcaggagcag cgccaggtct tccgccgtgc
     3841 acgccatcgg cccgccctgg tcgaggctgg aggcgaacgc gatcattccg tagcgcgaca
     3901 ccaccccgta ggtgggcttg agcccgcaca cgccgctcaa tgccgccggc tgtcggatcg
     3961 agccgccggt gtcggtgccg atcgcgccgg gcgcgagccg cgccgccacc gccgcggccg
     4021 atccccccga gctgcccccc ggcacggcgt ccacgttcca ggggttcctg accggcccgt
     4081 aatgcgaggt ctcgctggac gagcccatgg cgaactcgtc catgttggtc ttgccgaggt
     4141 tgacggcccc ggcagtgttg agccgctcga ccacgtgggc gtcgtaggga gacacgaagt
     4201 tggagagcat ccgcgacccg caggtggtga gccagcccct ggagcagaag atgtccttct
     4261 gggcgatggg gacgcccgtc agcggctggc ctgcgccggc ggcgcgcatc ccgtcggcgg
     4321 ctttggcctg cttcaggctg gcctcatcgt cgagcgtgat gaagcagttg tacttcttgt
     4381 ttaacacctt gatccggctc aggaaatgcc gtgtcagctc gacgctggaa agcttgcgcg
     4441 cagccagatc cgccgacaag cttttgatgg tggcgttgtg catctcaacc ccgggagggg
     4501 tgaggagaga gggatgagag ggagggttcg cagctacgca tcactcttca cccctcactc
     4561 cttactcctc actccatgac cttgggcacc agatagaggt cagcttcgac atggggggca
     4621 accgattgga acagcgcgtg ctggtcgccc tcggtgacgg cgtcctcgcg cagccgctgg
     4681 acgatgtcca gtgcatggga catcggcgcg accccgcggg tatccaccgc ctgcatctcg
     4741 gcgatgagcc ggaatacgtc gttgatttgc gacagcacgg cccgcgcttc ggcctcgtct
     4801 atggcgagcc gggccaggtg ggcaacacgc ttcacgtcgt ccagcgtcag cgccatgtca
     4861 cccccaggga gaaatgcctt aaaaatcgaa gctgcatagg gtatcataac cgccggacga
     4921 gaaacgccaa atccctttgg ccggaaagcc ttatgttcgg tttcctgagc agctacttta
     4981 acgatgatat cgccgtcgat ctcggcaccg ccaacacgct gatctacgtg cgcggcaagg
     5041 gcatcgtgct cgatgagccc tcggtcgtcg cgatacgcca ggacggcggc cccaacggaa
     5101 aaaaagtgat ccaggaagtc ggcctcgccg cgaagcagat gctcggccgc acgcccggca
     5161 acatcaccgc gatccgcccg atgaaggacg gcgtcatcgc cgacttcacg gtgaccgagc
     5221 agatgctcaa gcacttcatc aagaaggtgc acgagtcgcg cgtgttcaag ccgagcccgc
     5281 gcatcatcat ctgcgtcccg tgcggctcca cgcaggtcga gcggcgcgcg atccgggagt
     5341 cagcgattgg cgccggcgcc tccaacgttt atctgatcga ggagccgatg gcggccgcga
     5401 tcggcgccga cctgccggtg ggcgaggcga ccggctcgat ggtggtagac atgggcggcg
     5461 gcaccacgga ggtgggcgtg atttcgctgg gcggactggt ctacaagggc tcggtgcgcg
     5521 tcggcggcga caagatggac gaggcgatca tcaactacat ccgccgcaat tacggcatgc
     5581 tgatcggcga gaccaccgcc gagaacatca agaaggagat cggttccgcc ttccccggat
     5641 cggaagtgcg cgagaaggaa gtgaagggcc gcaacctcgc cgagggcatt ccgcgcagct
     5701 tcaccatctc ctccaacgaa atcctggagg cgctgaccga gccgctcaac tccatcgtct
     5761 ccgccgtcaa gtccgcactc gagcagaccc cgccagaact tgccgccgac atcgccgaga
     5821 agggaatggt gctcaccggc ggctgcgcgc tgctgcgcga catcgatcgc ctgctgatgg
     5881 aggagaccgg gctgccggtg atcatcgcgg aggacccgct cacctgcgtc gtgcgcggct
     5941 gcggcaaggc gctcgagaag atcgagcggc tctccagcat cttcaccaac gactagcgtt
     6001 cgagttttga atgtttagtg ttgagtgttg agtgagctcg gcatgaaggc tacaacccaa
     6061 cactcaaaac tcagcactta gcactgctcc gatggagcat cagcccccgc cgtttttcaa
     6121 gaccggcccc accccgctcg ccagactgct gatcttctcg acgctgtccc tcgcgttcct
     6181 cgtcgccgac gtccgcttca actatctcgg cacgttgcgc caggtcgctg cggtcatcgt
     6241 ctatccgttc cagcgccttg cgaccacgcc cgcgagcatt gcggggcgca ttggcgagtt
     6301 cttcgtcacc cacggttggc tgcgcgagga gaacgcacgc ctcgtccggc agaatttcga
     6361 gaacgcggcc ttgctgcagc aactggaagc gctgcaggcg gaaaacgccc agctgcgcga
     6421 gctgctcgcc acccgcagcc gcctcgacgt cgagctcgtc gccgccgagg tgctgtacgc
     6481 ggcgcgcgat cccttcaccc gcaaggtggt catcgacaag ggctctcagc acgaggtgcg
     6541 ccccgggcag ccggtggtgg acaatcgcgg cgtcgtcggc caggtgacgc gcgtctactc
     6601 ctggctcgcg gaggtcaccc tggtcaccga caaggggcag ttcgtgccgg tgcaaaatct
     6661 gcgcaacggc ctgcgcgcgg tcctcgcggg aaccgggaat gacggcgcgc tggagcttcg
     6721 cttcattccg ctcaacgcgg actttcagaa cggcgaccgg ctcgtaacct ccggcatcga
     6781 cggcatctat ccgccgggct tgccggtcgc gcaggtgacg cacgtcgagc gcagcgccga
     6841 gcagctcttc gcccgcatca cctgcgcgcc gctcggcggg gtggcaaacc atgcccacgt
     6901 gctgatcgtc cgcagtcagc gtgaaacccc gccgcggccg gtcgaggagc cgcagccgtc
     6961 gcggcccggg aaaccgagga aagactgatg gccgacgcac gcatcacccg ccacgtaatc
     7021 ctgctgcccg tcaaggtcgg cttcatcgtt ttctcgctga tcgcggcgtt gctgctgaat
     7081 cttctgccgt ggtcgggcgc ggcgctgtgg atcaagcccg acttcgtcgc gctcgtggtg
     7141 ctctactggt gcatcgagca gccgcgcaag gtcggattcg tcgcggcatg gctgctgggg
     7201 ctgttcatgg acgtcgccga cgggacgttg ttcggccagc atgccctggc atactccatc
     7261 ttggcctacg cgggcatcgt gctgaatcgc cgcgtgcgca tgttttcggg aacgccgcag
     7321 gtgctgcacg tcgtgatcct gctcctgatg aacgacctca tcgtgatcgg catccgcgcg
     7381 gccgcgggag cggacttccc cggattccag tacttcatcg gcagcttcgt cgcgggcgcg
     7441 ctgtggctgc cgctcggcgt gctgctcaag ctgccgcagc gccccaggcc ggatcccgat
     7501 catgtctgag ctgtcgatca atcccggcat cgagctgcgc aatgcgcaac tcgagctcca
     7561 ccatttccgg gtgcggctgg cgatcgcggc gggcttcatg ctcgccatgt tcctgctgct
     7621 tgccggccgc ttcttatacc tgcaagtcat caagcacggc gagctgcaga cgctcgcgga
     7681 ggcgaaccgc atctcgatcg tgccggtgat gcccaaccgc ggcatcatcg tcgaccgcaa
     7741 cggggccgtg ctcgcgcaca actactccgc ctacacgctg gagatccagc cgtccaaggt
     7801 ccgcaacctg gatgcgctga tcgacgagct cggcaccgtg gtcgagatca cgccgcgcga
     7861 ccgcaggcgc ttcaagcgcc tgctcgagga gagcaggagc atcggcagcc tcccgatccg
     7921 cacccgcctc tcggacgagg agatcgcgcg ttttgcagcg aaccgctacc gtttccccgg
     7981 cgtggaaatc gacgcacggc tgttccggca gtacccccag ggcgaggtct tctcccacgt
     8041 ggtgggacac atcggccgca tcaaccagcg cgaggtcgag gagctcgagt cagagggcca
     8101 tgccgccaac taccgcggga ccgattacat cggcaagacc gggctcgagc agaactacga
     8161 gcgctacctg cacggcacca ccggcgtgga ggaggtcgaa gtggattccg gcgggcgccc
     8221 ggtgcgcacg ctctcgcgca cgccgcccgt ctccggcaac aacctcgtgc tcaacctcga
     8281 cgcgggcctg caggaggccg cctatctcgc gttcggcgac cggcgtggcg cgctggtcgc
     8341 gatcgagccc ggcacgggcg gcgtgctcgc cttcgttagc aagcccggct tcgaccccaa
     8401 cctgttcgtg gacggcatcg atccccagaa ctggagcgag ctcaactcct cgcccgaccg
     8461 cccgctcgtc aatcgggcgc tctcgggcac ctaccccccc ggctctacct tcaagccgta
     8521 catggcgctc gccgccctga cctacggcaa gcgcacgccg cagcagacga tcaacgatcc
     8581 cggctacttc atgtttggcg gccaccagtt ccgcgacagc cggcctggcg ggctcggcgt
     8641 cgtcgacatg tacaagtcga tcgtaatgtc cagcgacacc tactattaca tgctggcgaa
     8701 cgacctcggc atagacgcga tctcgcgctt catggggacg ctcggttttg gcagccgtac
     8761 cggtatagac atcctgggcg aggcgaccgg ggtgctgccg tctcaggagt ggaagatgcg
     8821 ccgcttcaag cagaagtggt ttgcgggcga gaccatcagc atcggcatcg gccaaggcta
     8881 caacgcctac acgccgctgc agcttgccca ggcgatcgcc acaatcgcga acgacggcgt
     8941 ggccttccag ccgcacatcg tcaactacgt cgaggacatc cgcacccgcg gccgcaccgc
     9001 catcgagccc cgcccgctgc gcacgctcga cgtgaagccc gagcatctcg cggtcatccg
     9061 gcaggcgctg gtcggcgtca acaaggaggg caccggcgcg cgcgccttcg cgggggcggg
     9121 gtacgtgagc gccggcaaga ccggcaccgc gcaggtgatc ggcatcaagc aaggggagag
     9181 gtacgtggaa agccgcgtgc aggaacgcaa ccgcgaccac gctcttttca tcgcctacgc
     9241 gccggccgac aacccgaaga tcgcgctcgc cgtgatcgtg gagaataccg gtttcggcgc
     9301 gcgctacgcc gcgccggtcg cgcgcaaggt gttcgactac tatttcctcg gcaagctgcc
     9361 cgagccggac aagcgcggca aggacgacga tgaatccggc aattagacgc ctcgagtact
     9421 acctgtcgcg caacgttgac ggggtgctgc tcggcctcac agtcgcgctg atgatcgtcg
     9481 gtctggttac gctgttcagc gcctccaacg tgaatgcggc gcgtctcaca gcgcagctcg
     9541 ccaacatgct ggtcgcgctc ggcgtgatgt ggctgttcgc caatatcccg ccccattacc
     9601 tgatgcggct cgcgtttccg ctctatgcga tcggcgtgct gctgctcgtg gccgtggccc
     9661 taggcggcga catggtccac ggcgcgcggc gctggctcga cctcggcatc acccgcatcc
     9721 agccctccga gctcatgaag atcgcggtgc cgctcgcgct cgcgtggtat ttcgaacgcc
     9781 acgaggcgac gctgagactc accaactacg cggtcggcgc ggtgatgctg ctgataccgg
     9841 tggcgctgat cgcgcggcaa cccgacctcg gcaccgcgct gctgatcacg gcgtcgggct
     9901 tcttcgtgct gttccttgcc gggctttcct ggcgcatcat tggcgggctc gccgtcgccg
     9961 cgcttgccgc agctcctttc gcgtggtccg cgctgcacga ttaccagcgg caacgaatcc
    10021 tgacgctgct cgacccgact caggacccgc tcggctccgg ctaccatacg atccagtcca
    10081 ccatcgcggt cggctccggc ggctggttcg gcaagggctg gatgcaaggc acgcagtccc
    10141 atctcgactt catccccgag cgcaccaccg acttcatctt cgcggtctat gccgaggagt
    10201 tcggcctgtt cggctccctc ttgttgctcg gacttctcct gctcgtgatc tcgcgcggca
    10261 tggtcatcac cgccaacgcg ccgacgctgt tcacgcggct gctggccggc gcgatcacgc
    10321 tcaccttctt cacctacgcc ttcgtcaaca tgggcatggt gagcggcatc ctgccggtgg
    10381 tcggcgtgcc cctgccgctc gtgagctacg gcggcaccgc actggtttcc atttgtctcg
    10441 gcctcggaat actgatgagt atccagagcc acaagaagct tgtccaaacg tgataggtga
    10501 caagtgatag gtgataggaa aaacgccctt cccggcgcgg agcgccggaa aaacacttat
    10561 cacttatcac ttatcactta tcacctcgcc gccgcgttgc ttgcggcagc gctgggcgct
    10621 tgcggaaccg tgtccaagcg tgatgcctct gccaaagcgc cgcctccccc gcgcggcggc
    10681 ggttactacc tcgacgacgg ccccggcgcc aatccgcctg ccgatctgga cggcgtcccc
    10741 gacgcggtac ctcggctcga accgcttcat cgcgggacga gccggccata cgtagtgatg
    10801 ggcaggcaat acacgccgat gacctcgctc cagccgtaca gggcgcgcgg catcgcaacc
    10861 tggtacggcc ggcgttacca cggcaagcag acttcctcgg gcgagattta cgacatgtac
    10921 gccatgaccg ccgcacatgc cacgcttccc atcccgagct acgcgcgcgt gaccaatctc
    10981 tccaacggaa aatccgtggt ggtccgcatc aatgaccgcg ggccctttgt cgacggccgc
    11041 atcgtcgatc tctcgtacac cgccgcccac cggatcggcg tgctgggcgg aggcagcgcg
    11101 atggtggaag tggaagccat cattcctgac gggaccggcc caaccgcggt cgcgcatgcg
    11161 ccgcggccgg cgggcagcag ggttgcggcc gcggcggcgg caccggtcga ggcgcccgcg
    11221 gccggcgacg cgatgaccgc gagccgcgcg tccgagccgg aagcgcagcc actgcccgcc
    11281 gccgaagcgc aacctgcggc agcggcggcg acgcaaatac cggtcgccgc tgaatcaggt
    11341 ggctactacc tgcagctcgg cgcgttcggg tcaagggaaa acgcggagaa tttcctcacg
    11401 cggatgaaag cgcaggtgga atggctcgca tcagcgcttc acatcttcac ccgtgatggg
    11461 ctgttccgcg tgcacgcggg cccctacccc aatcagtccg aggcccgcca gaccgccgag
    11521 cgcatcggcc aggcactggg cattaaggcg atggtgctcg tcaggtaagc agtaagcggt
    11581 aggcggctca ccgtcactgt tttccttcca accgcaacaa agcgtgaacc atttccagca
    11641 ccatccgctt gtgcgcatca ttcagcagct caaacttcct tgacggccag ccatagtcca
    11701 gcctgtagga accgctctcc tgccgcgccg ccaaaggttg ccgttcatct ttcctttcgg
    11761 cttcgccaaa gcgcaaccaa tgcacggata cttcgagcca taacccgaga gcacggattt
    11821 tgtcctgtga aggcaaggcc tttccggata tccatttccg cactgcctgc gtggaaaccg
    11881 ggtcaccgtg ataacgcaga ttgaactcgc gggcgatcag cgtcggactg ctgatatcga
    11941 tgcgcgcttt gcgcatcgct tccttaagac gcttgctgaa accctgtttc tcatcagcga
    12001 gagtcatgct tggaacagta tcaccaagca caacgacata tggttaccgt tggttgcgtt
    12061 ttacgacaac cgggagtagc atgtcgccct gccctagcgt atttccgtta agccttttga
    12121 tatcgcggtc cagtccgcat gacttggcgc gaacgagacc tttccaaggt catcgagcat
    12181 ataccgctcg gagtggcgat caccctgccg gacggcatgc tcgaatacgc caacccgtat
    12241 ttctgcaagc tgatcggcgc gttcggcagt gaactggtcg ggacgccgct ggcccgctgg
    12301 ctatcaactg acgcaccccc ggatcgcggg gcgaggcagt cgataagctc cgggcctgcg
    12361 tggcgtgggg aaacgcatgt ccggctcagc agcggagagt tgctcgatgt tctggaggcg
    12421 gtctatccgc tgcatgacaa agcgggaatt ctcacacacc tcatccacct ggtgcaggac
    12481 atcagcgcgc aaaaacaact ggaactcctg tccgcgctgg ccttctacga cggcctgacc
    12541 gggctgccca accgcaatct gttgaacgac cgtctgacgc gtgtcatcgc cggagcgcgg
    12601 cgccactacg gcgccttcgc gctgctctac atcgatgtcg accacttcaa ggagatcaac
    12661 gacacgcagg gccatgacgc cggagacgaa ctgctgcgtc agcttgcggc gcgcctctcg
    12721 cagtcgctgc gcgcagccga taccgtgggc cgctggggcg gcgacgagtt tgtggccatg
    12781 cttgacggag tggcaagtcc gcaagccgcc acgcgcatta tccgcaagtt gctttcgatc
    12841 tgcagcgagc catatgatct gggcagcggc gtctgctccg tcacgctcag tatcggcgca
    12901 agtctcttcc cgcgcgacgg gtacgacatc ccaacccttc tgaagcgcgc ggatgaggcg
    12961 atgtacgccg tcaaaaagcg cggccgcaac ggctaccacc tgctgaagga acccggcagc
    13021 cgctaccgga aaacggttca aaaagaaccg gccatgatca cctaaagaac gtagcaggct
    13081 gtcgattcac agatcacaca tcacggttca ctgatttccg tcggatcacg gatcacagat
    13141 cacggctcac gtcgtcaaga agcgccctcg agccggaata cgatcggcac cagcacccac
    13201 gactcgatcg cttccgtacc ccggcgcgcc ggcacgaaac gccaggcctt gaccgcttcc
    13261 agcgccgcgg cgtcgaggtg ggacgagccg ctcgatttct cgacgtccac gcgcgccggc
    13321 agcccctcgc gcgtcaccag cacgcgcagc gtcaccgtac cttgctcgtc ggcgcgccgc
    13381 gcggcgaccg gatagcgcgg cgccgggttg ctcagataag cggcgttgaa ggtcggtggc
    13441 gtcatggttg cgctcgccac ccgggatctc tgctcgggca cggcgaccgg cagctccggc
    13501 atgctgtcag gcggaaccgt gaacgagcct tccgtcgccc gcggctccgg caacgccagt
    13561 atcggcgccg tacgctcagg ttgcggtttc ggcaacgccc tggccggcac gcgctccgct
    13621 ccgctcaacg gagcaggttg cggggtggat tcgggaagcg caacgggcag cggctcgggt
    13681 tgcagtatca ccacttcgag cgcgttcacc ttgggctgct cgcgatcggg tgaaaacccg
    13741 ggtaacgcga ccaataacgc ggcatgcgcg gccacgctgc cggcgaggaa cgcgccgagc
    13801 aggcgccgcg cgttacggcg cgccgccggc gcacgagcat cgaggttgtt ctgaccgaag
    13861 aaagccgtca tgccaagccc cggcaaacgg gctcgatgag tgcccgattc gcctctgtcc
    13921 ggcgaattgg atcaggagct actgcgcgac cgccaccgcg tcggtcatgc ggtagtagag
    13981 tcccatcggg tcgtccttca gcacggcgaa cttgccggag acgacgatgg gatcgaagcc
    14041 atacttgacc ggggtcttgg ccaccacctc gaccagctgg tcgggaccac ccggcaagca
    14101 gaaagcgcac gagggcggca gcgcgacgag caggaaccgc ttctgtttct cgcccatgtc
    14161 gagcggcatc atgaagccct ggagcttaac ttccttctgg tcgagcgcgg aaacgtcctt
    14221 cgaaaactgc ggcacgaagc ggtccttctg cttgatcggc gtgactgccg cgagcgtctt
    14281 ccacgacacc acgcccttca gctcgggtag cggcttgtcc aagtagggag aaggcggtag
    14341 cggagcgtcc atgggtccca tgtccttgcc gggaacaccc ttttgctgcg cccaggccgc
    14401 gtgggagacc agcagcatcg cgaaaacata gacggcgaat ttcatgtttt tctctccatg
    14461 gtccctggcc cgcccacccg ggccatcgct tcggaacgct gattctaccc gaaagttccc
    14521 ggatcgggtc gcggagcagg tcaatgcctg tgcttcatcc cgcaatactt gccgattccc
    14581 agccccttgc agtccttggc gagctgagca tggcactcct tctccggtct gccggactca
    14641 aggcatttcg cggcgttgac atgcgcctcc gccatggcaa ggtgatctgc aacggcctgc
    14701 ttcacgtgcg ccggatcgtc cttcttgccc ttctccgccg caaacgcggc cggcacagcc
    14761 aacaggcccg ccgtcatgac aacaatcatc agggctctca tggtttgcct ccttcgctca
    14821 tcactcatca ttcatcactc atcattgatc ttcacccccg cgccagcgta tgcgcgatgt
    14881 cggtgcgata agcgcgccag gccggcaaca gcgcggcaac aacgccgacc acgagcgcga
    14941 gcccgaacag ccagagttcc ccgggaaccc agacccaacc ggtcaccgcc acctgttgcg
    15001 cggctttcag cgcgtagccg agggcctcgg tcacggcgtg acccagagca agtccgagca
    15061 cggcgccgag gcccgcgagc agcgcgcctt cgaacagcat cagcatcatg agcctcagcg
    15121 ggctcgcgcc cagcgtgcgc atcagcgcca gatcgtagcg gcgctcggcg agcgcgttgt
    15181 agagcgcgat gaagaggctc aaccccgccg agaagatcag caccagcgcg aagccgcgca
    15241 gcacgtccac gcccacggcg acgatgctga acaggcgcgc ggtctcgtaa gcgggcgagg
    15301 cggcctgcat ttcgctgttc tgattgatgt agcgcggcag cgccgcggcg gcgatcggcg
    15361 tcgcatactg gatcagcagc gcggtgattt ccttgtcctc ttccgtcacc ttcgcatcgg
    15421 tccccatgtg ctcgtcgtgc accagccaga cgctctcgat gctggtcagc accagccggt
    15481 cgagcacggt gccgctcggc gcgagcacgc cgaccacttg gtagggcgcg tcgccatgct
    15541 ccgcgccggc ctccgcgatg ccatgcgcgc cggtgaatgt agccccgacc gcgtgcccca
    15601 tgcgttgcgc cacatcggcg ccgatcaccg tttccatcgg cttttgccac agcctgcccg
    15661 ccgcgagctt gccgtcgtag tgcgcgacgt aatcggggtt cgtgccgacg atgcggaagc
    15721 ccttgtagct gtcgcccagc gcgagcggaa tggtcttctt gatcatccgg tgcttcgcga
    15781 tttccttcgc ctgggcgagc ggaatgttgc cggtcggcac gtcgaggtga taaatcgctg
    15841 agagtatcac ctgcatcggg ctgcccttgg cgccggccac gagatcgatg ccgcgcgcgt
    15901 cgcggcccat gcgctcctcg agttgggagg tggtgagcag caacagcgtg atcgtcgcca
    15961 ccccgagcgc gagcagcgcc acgttgagcg cggtgttgag cgcgcgtgcg cgcagatagc
    16021 cgaagctcaa cttgaagagg ttcatcatcc gcctccgagc gcgaactggt cgggcatgcg
    16081 cgacttgacg cgccggtcgt gggtggcgat caggagcgtc gcgttgcagg cgcgcgcctg
    16141 ggtttgcagc aattcgagcg cctgcatgca gcgcccgtcg tcgaggttgg acgtcggctc
    16201 gtccgcgagc aggagctgcg gacgattcac caccgcgcgc gccaccgcca cgcgctgcgc
    16261 ctggccgaag gaaagctcgt gcggatacgc tccttccttt ccggcgagat cgaggccggc
    16321 gagcacttcg cgcacgcgcg cgctgtcctg cggtagtccg gcgagatact gcgcgagcag
    16381 gaggttgtcc atgacggtca ggctcgacat caggtgcagg cgctgcagca cgatgccgac
    16441 atgctgcccg cgaaaccggt cgagcgcctc gggcttgagc gcgccgatat cctgccccgc
    16501 gacgctgacg ctgcccgcgg tcggcttgag gatcccggcc aggatgtgca gtagcgtggt
    16561 cttaccgctg ccggaggggc ccagcaccag ccagtgcgag ccctgctcgg cctgccacgc
    16621 gggcacgttc aggacctccg tcccgttgta ggcgtgcctc agattttgta tggcgaacat
    16681 gtgacttgct gcccgcggcc cggcaaagcg cacgcattct actcgtaccg gcgcaagcgt
    16741 gctcgcgcag tatactgccg ccgcatgaag tggctattgc tgcgcctgat cggcgcttat
    16801 caattgctcc tgtccccctt cttcgggcag cagtgccgct tctacccgac ctgctcggcc
    16861 tacgcacgcg aagcgatcga ggtgcacgga tcgctgaaag gctcctggct cgcggtgaag
    16921 cgcatcctgc gttgcgggcc gtggcatccc ggcggcaccg atccggtgcc gcccaaaggc
    16981 aaaagtcgaa aggcgaaagg cgaaagttaa ggcaaagctg ctcagcgggt ttccctttcg
    17041 cctttcgcct ttcgactttc gactttcgac ttggctcgtc agcccgcaac gcggcagacg
    17101 agccctttca ggtattcccc ttccggaaag ttcaacgcca ccggatgatc ggcgccgggg
    17161 ccaagccgtt cgatgatctg cgcgtccacg ccggcatcga gcgccgcgcc ggcgatgatc
    17221 ttctggaaaa gatcgcttga gacgccgccc gagcacgaga aggtcatcag caggccggcg
    17281 ggacgcagca gcttgaacgc cagcagattg atatccttgt aggcgcgcgc ggctttcgca
    17341 gcgtgggcgg cggttggtgc gaacttgggc gggtcgagta cgacaagatc gaatgatctt
    17401 cccccatcgc gaaacttccg cagcagctgg aatatattgc cctcgatcca ctccacgccc
    17461 gcaaggtcgt tcaacttcgc gttggcgcgg gcgagttcca gcgcttccgc ggaactctcc
    17521 accgccgtga cggatcttgc cccgcccgct tgcgcgttga gcgcgaagcc ccccgtgtag
    17581 cagaagccgt caagcgcgtc gcgcccgcgc gcaagctccc gcaacctccg gcggttatcg
    17641 cgctggtcga gatagaaacc ggtcttgtgc ccgtgctcga ggtcgatctc gaaccggagg
    17701 ccgttttcgg tgaccgcgat ccgc
//
LOCUS       DBNGWZ010000138         4167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_643020, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000138 DBNGWZ010000000
VERSION     DBNGWZ010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_643020"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC16CX_04170"
     CDS             <1..267
                     /locus_tag="AC16CX_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR3011742.1"
                     /translation="RFGLFVHLLTLPGEAPELSGRVSLWIGIAFILIGCAVSTVAVLQ
                     YRKVLLTLKPVEIPQGYRVNLGVFTNLAVAVLGVALVLYLMQGT"
     gene            304..789
                     /locus_tag="AC16CX_04175"
     CDS             304..789
                     /locus_tag="AC16CX_04175"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR3011743.1"
                     /translation="MKQSIIIFLVGGVLGAAAGFALGIFVYPYFFLADIVAAEKVENT
                     ASRKAVATGMFIHANPRDPIHYGKGKVTVYQDLVHLGGDFEVGPGPKFHVYLVPEKNV
                     VPSTNVAKTMYVDLGRLRAFKGSQNYPVPDGVDLTKYDTVVIWCEQFGVLISPATLKF
                     Q"
     gene            825..1286
                     /locus_tag="AC16CX_04180"
     CDS             825..1286
                     /locus_tag="AC16CX_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR3011744.1"
                     /translation="MMERLHRLLESADRRLGAGQRTVRAALILLAITVTVALAAVAWP
                     IVVELHSERITAGSREAYLNRVSEKILRAMGSAMATRPKQDGAESLQLRIAVERDGGL
                     ISAEIVQPSGNRALDELALRIVRESAPFEAFPAEMRGGTTSVEIISTFNFH"
     gene            1770..2150
                     /locus_tag="AC16CX_04185"
     CDS             1770..2150
                     /locus_tag="AC16CX_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3011745.1"
                     /translation="MVKHTGGCHCGRVRFEVLAPARIRVSECNCSICSKSGYLHLIVP
                     ADRFTLLSGREVLSTYTFNTGTAKHLFCSVCGVKSFYVPRSYPDGFSVNARCLDPGTV
                     EEMTVTQTDGKNWEKQYPSGRGKY"
     gene            2336..2836
                     /locus_tag="AC16CX_04190"
     CDS             2336..2836
                     /locus_tag="AC16CX_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011746.1"
                     /translation="MEAESILGVYAQLAVVIVGLSGIVGVLGHRATGNWEIADYTRFW
                     TMMASSFSLLFQAIFPIILHHFSLTPQAVWSWSSAAAALVVGGQLYWRFGNLKRAHTD
                     PNFHVYVWWFNNILQIAAAVILILNWAALGFEGTFAPYLLAMSLTLAVSCVTFVRLLI
                     TALPRH"
     gene            complement(3004..3849)
                     /locus_tag="AC16CX_04195"
     CDS             complement(3004..3849)
                     /locus_tag="AC16CX_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HIR3011747.1"
                     /translation="MVCAATAAQTISVTDDRGKIVTLARPAQRIVALAPSLSEIAFAA
                     GAGGRLVGVARFSDFPPGARNLPQVGDAARIDLERIVALKPDLVLAWSSGNPQRDVAR
                     IEALGYPAFVSEPARLPDIPRLLRAIGSLAGTRGAAEKAARDFEREIGELRRRHAGAP
                     KVRVFYQIWHNPLLTVNGAHIISDVITLCGGENVFSDVSQLTPAVSLEAVIAARPEAI
                     LGGGSAGGEEQFAAHWREMAAQPLRQLPAYYVNPDLIQRQTPRIVEGARAVCAALEQV
                     RSSRR"
BASE COUNT          811 a         1233 c         1295 g          828 t
ORIGIN      
        1 cgcttcggcc tcttcgtgca tctgctgacg ctgccgggcg aagcgccgga gctgtccggc
       61 cgcgtctcgt tatggatcgg cattgccttc atcctgatcg gctgcgcggt ttcgaccgtg
      121 gcggtgctcc agtaccgcaa ggtgctgctc acgctgaaac ccgtcgagat tccgcaaggc
      181 tatcgcgtga atctcggggt ctttaccaac ctggcggtgg cggtcctggg cgtcgccctg
      241 gtcttatact tgatgcaggg cacctgacgc ccgccatcga acgcgacaag gacgcggacg
      301 gcaatgaagc aatccatcat tatttttctt gtcgggggag tgctcggcgc cgcggcggga
      361 ttcgcgctcg gcattttcgt ctacccttac ttcttcctcg ccgacatcgt cgccgcggaa
      421 aaagtggaga acacggcatc gaggaaagcc gtcgcaacgg gcatgttcat ccatgccaac
      481 ccgagggacc cgattcacta cggcaaggga aaagtgaccg tctaccagga tctcgtgcac
      541 ctgggcggcg acttcgaggt ggggccggga cccaagttcc acgtttatct ggtgcctgaa
      601 aagaacgtcg ttccctcgac caacgtcgcc aaaaccatgt acgtggacct cgggcggctc
      661 agggccttca agggcagcca gaattatccg gttccggacg gcgtggacct cacaaagtac
      721 gacaccgtgg tgatctggtg cgagcagttc ggggtgctga tttccccggc tacgctgaag
      781 tttcagtaat caatcagcgg ccaagaaagg caaataaaaa aacgatgatg gagcggctgc
      841 accgcctgct cgaaagcgcc gaccgccggc ttggcgccgg gcagaggacc gttcgcgctg
      901 ccctgatcct gcttgcaata accgtcaccg tcgcgctcgc ggccgtggcc tggcccatcg
      961 tggtggagtt gcattccgag cgcattacgg cgggctccag ggaggcttac ctgaaccgcg
     1021 tcagtgagaa aatcctgagg gcgatggggt ctgcaatggc gacgcgtccc aaacaggacg
     1081 gggcagaatc gctgcagctg cgcatcgcgg tggagcggga cggaggtctg atttccgccg
     1141 agatcgtgca gccatcggga aaccgcgcgc tcgacgagct cgcgctgcgc atcgtcaggg
     1201 aatccgcgcc attcgaagcg ttccccgcgg aaatgcgcgg gggcacgacc agtgtggaaa
     1261 tcatcagcac gttcaacttc cattaaatcc ggacgcacag cgccggttta agtagcgcgt
     1321 cggagggcgg aacagggccc ccgtacaccg gggatgtgac cccggagacg ctcaggcggc
     1381 cgacacgatt tccctcgatg cttgccgata gccacccaga ggccgccgcg cgttcgaaat
     1441 gcaattaaat ccggacgcga cgcgccggtt tgagtgcgcc ggaaggttga gcaggtgccc
     1501 gcttgcgggt gcgacccaag gagggcacgc cggagcgtag agcaggtgcc cgcttacggg
     1561 tgcgaccgca taggcgtgca aggcggccga cacgatggag cagaattatg ccgatggctg
     1621 atcggaggct gcgcgcatgg cggccggcgc gtacagctcg gctcctgtga tgaccgttcg
     1681 aaggccgcgc gcgttggagc ggacgatttc atcgccgttc aacgcgcggc aatctcgact
     1741 acactagctc ttctgcgagg gaattccata tggtcaagca tacgggtggc tgtcactgcg
     1801 ggcgggtgcg gttcgaagtg cttgcgcccg caaggatccg ggtgtccgaa tgcaactgct
     1861 cgatctgcag caagtcggga tacctgcacc tcatcgtgcc cgccgaccgc ttcactcttc
     1921 tcagcgggag agaagtgctg agcacctata ccttcaacac cggaaccgcg aagcacctgt
     1981 tctgctcggt gtgcggcgtg aagtcgttct acgtgccgcg ctcgtacccg gacggcttca
     2041 gcgtcaacgc gcgctgcctc gatccgggga cggtcgagga gatgacggtc acgcagacgg
     2101 acgggaaaaa ttgggagaag cagtatcctt cggggcgggg aaaatactga gggtgacgca
     2161 gtgaccaggt gacgatgcga cgaagtgacg attcgccgag gttttgagtt tatatgctgc
     2221 ttcacccggc agtatcttga ttcagttgag agatgacgtc gtcaagcaca caataccaat
     2281 tttcaaatcg gctattgggc caaccacttt acgtcagacc gcgggcatgt agaaaatgga
     2341 agccgaatcc atactgggtg tctatgcgca gctagcagtc gtcatagttg gtcttagtgg
     2401 aatagtcggc gtactcggcc accgagcgac tggtaattgg gaaatcgccg actatactcg
     2461 gttctggaca atgatggcat ccagcttttc attattgttt caggcaattt tcccgatcat
     2521 attgcatcat ttttcactta ctccgcaggc tgtttggagt tggagcagcg cagcggcagc
     2581 tctagtcgtc ggtggccaac tgtactggag atttggaaac cttaaacggg cacacacgga
     2641 tccaaatttc catgtatacg tctggtggtt caataatatt ctgcaaattg ctgcggctgt
     2701 catccttatc cttaattggg cggctctagg attcgaagga acatttgcgc cgtacctgct
     2761 cgccatgtct cttacattgg ccgtatcgtg cgtgacattt gttcggttac tcatcacagc
     2821 gttgcctcgt cactgatcga gaaagtagat atcgccggtc tgacaatgct tgatcggtca
     2881 cgcaagagac cgcgcatcgt ctagcttaaa tgttaggttt tctgatcttc ggatctctcg
     2941 gtgtctctgc ggttcaatta ttttccgatt ttcttggcgg ctctgaattg atttgatcgg
     3001 cgtctatcgg cggctgcttc taacctgctc cagcgcggcg cagacagcgc gcgcgccctc
     3061 cacgatccgc ggcgtctgcc gctggatcag gtccgggttg acgtagtacg ccggaagctg
     3121 gcgcagcggc tgcgccgcca tctcacgcca gtgcgcagcg aattgctcct ctcctcccgc
     3181 cgagccgccg cccagtatcg cctcgggcct tgccgcgatc accgcttcca gcgacaccgc
     3241 cggcgtcaat tgggacacgt ccgaaaagac gttctcgccc ccgcagaggg tgatgacatc
     3301 gctgatgatg tgcgcgccgt tcacggtcag gagcgggttg tgccagatct ggtagaacac
     3361 gcgcaccttg ggcgctcccg catggcgcct gcgcagttcc ccgatttccc gctcgaagtc
     3421 cctggccgcc ttttccgcgg cgccgcgcgt ccccgcgagc gagccgatcg cgcggagcag
     3481 ccgcgggatg tcggggaggc gcgccggctc gctcacgaac gccgggtagc cgagtgcttc
     3541 gatccgggcg acgtcgcgct gcggattacc gctgctccac gcgaggacga gatcgggctt
     3601 gagcgcgacg atgcgctcga gatcaatacg cgcggcgtca cccacctgcg ggaggttgcg
     3661 cgcgcccggc gggaaatcgc tgaaccgcgc gacgccgacc aggcgacccc ccgcaccggc
     3721 ggcgaaagcg atttcggaga gactgggcgc gagcgcgacg atgcgctgcg cgggccgcgc
     3781 gagggttacg atcttgccgc gatcatcggt gacggagatg gtttgtgcgg cggtcgccgc
     3841 acaaaccatg aagaaaaccg gaatgaagcc gccgatacac gccgatagac gcggataaca
     3901 acgaaacggg acggccagaa agaccaaagg atgacgggca gattcttgaa ctacaggggt
     3961 caggatcgag gaaggccggc ctgcatcacc agcgttgggt ttattccttc atccctccgc
     4021 cctcatccct catcccttaa acagcgctgc gctccatgta ttgcagcagc ttggcgctgg
     4081 tctggcgcag ccgcgcggaa agcatggaaa cgagcttcac caggatcttc gagcccaggc
     4141 tcggatggtc gaggatgatt ttcgcca
//
LOCUS       DBNGWZ010000139         4145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2910330, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000139 DBNGWZ010000000
VERSION     DBNGWZ010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2910330"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..558)
                     /locus_tag="AC16CX_04200"
     CDS             complement(100..558)
                     /locus_tag="AC16CX_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3011748.1"
                     /translation="MNAGLRSRIEAFLQEHHVMSLATSGPEGPWSAAVFYASDGLTLY
                     FVSAPESRHSRDIEASGCAAATIHRDVGDWREIRGIQLEGEAARVGGTERAAALARYG
                     RKFPLIAYLSSAPPEIAAAMARVEWYRLSPRRVRLVDNTLGFGHREELDL"
     gene            792..2660
                     /locus_tag="AC16CX_04205"
     CDS             792..2660
                     /locus_tag="AC16CX_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3011749.1"
                     /translation="MRLLLINPRFNESFWSFKWALDKMLSGKRAMNPPLGLATLAALC
                     PPDWKVEVVDENIESLPLAPQADIIGICGMGVQAPRQKDLLCFYRSKGYYVVAGGSYA
                     SLCPEFYDGIADTVICGEAEYTWRRFCNDFERGAARKLYAETGTVSMADSPTPRFDLL
                     KLEKYSVVTLQFSRGCPYLCEFCDIIVMFGRKPRWKSPEQIGLELDELRKRNIRDAFF
                     VDDNFIGNRKAARALLKYLKDYQERHRYRFIFGTEVSLNVAQDDELLRLFREANFAWV
                     FIGIESPDVDSLKETKKSQNLQNDILDSVRKIYARGIDVLAGFIIGFDNDTLGTFDRQ
                     YRFIVDSGIQAAMVGLLTALPRTPLYQRLEKEGRLIAQAESTNNTRLGTNIAPKRMEY
                     DAMVASYKALYTRLLEDRQIADRVRNKARYLRDPARQSQYGLIEGLGILGRLMARGIL
                     AGGWSRLFHFLRSLPLASPRLLPQAITDWIMGLAMRDYVERRFGVAMKTRQVAAVRLV
                     ESLQAALAVYLRSGKVIVALKGGPAAGLNLFVSLRGWLDRAFFSRAASHLEKLLKRTP
                     ATVTLRIELAPEAEISQLNRLLRRLAPYGDRIFIHVDAKLRELVRIDSSVFHLILE"
     gene            2657..2797
                     /locus_tag="AC16CX_04210"
     CDS             2657..2797
                     /locus_tag="AC16CX_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3011750.1"
                     /translation="MTACLSPFCEDEPQCKLVCPSNCILEHSDFRESRDELLAKYHSL
                     DG"
     gene            complement(3790..>4145)
                     /locus_tag="AC16CX_04215"
     CDS             complement(3790..>4145)
                     /locus_tag="AC16CX_04215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3011751.1"
                     /translation="REIRRSPRVAISMPFFYQVIENDVALPHVHQGTTLNIGYHGVLA
                     ETDQELTSSSELKLALDLPLVGSKATDIYAKIVKTERTEKGYLSGLEFTSLTTQDSRH
                     LQLFVQLLIQGSESQ"
BASE COUNT          811 a         1236 c         1235 g          863 t
ORIGIN      
        1 gcagtgcaag ctggtctgtc cgtcgaactg catcctcgag catcccgact tccgcgagag
       61 ccgcgacgag ctgctggcga agtatcacag cctgcacggc tagagatcca gctcctcgcg
      121 atggccgaag ccgagcgtgt tgtccaccag ccggacacgg cgcggcgaca ggcgatacca
      181 ctccacgcgc gccatggcgg ccgcgatttc cggcggcgcc gaggacaggt aggcgatcag
      241 cggaaatttc cgcccgtagc gcgcgagcgc tgcggcgcgc tcggtcccgc cgacgcgggc
      301 cgcctcgccc tcgagctgga tcccgcggat ttcgcgccag tcgccgacgt ccctgtgaat
      361 cgtcgccgcg gcgcatccgc tcgcctcgat gtcgcggctg tgccggcttt ccggcgccga
      421 gacgaagtag agcgtcaggc cgtcgctcgc gtagaacacc gccgccgacc aggggccctc
      481 cggccccgag gtcgcgaggc tcatcacatg gtgttcctgc aggaacgctt cgatgcgcga
      541 gcgcagcccg gcattcattt ctcccctttc cggcgcttgc ctcagacctc agggtcaggc
      601 ctttacctta acttggcccg ggacagctta gtctgtccct ggttttctgg tcttgatccg
      661 ctgttgcatc caggaaatcc cgcccaggtg gcccagatac catatgtgcg gtatcgtaca
      721 gatgactttg gaaccgggag agcagtatat tcccacgaac agccggcgga acgcataacg
      781 gtcgatcgac catgcgcttg ctcctcatca acccgagatt caacgagagt ttctggagct
      841 tcaaatgggc gctggacaag atgttgtccg gcaagcgtgc aatgaatccg ccgctcggcc
      901 tggccacgct ggccgcgctt tgcccgcccg actggaaagt cgaggtcgtg gacgaaaaca
      961 tcgaatccct gcccctcgcg cctcaggccg acatcatcgg catctgcggc atgggcgtcc
     1021 aggctccgcg acagaaggac ctgctgtgct tctaccggtc caaggggtat tacgtggtag
     1081 ccgggggcag ttacgcgtcg ctgtgtccgg agttctatga cggcatcgcc gacacggtga
     1141 tctgcggcga ggccgaatac acctggaggc gattctgcaa tgatttcgaa cgcggtgcgg
     1201 cgcgcaagct gtatgcggaa acgggcacgg tatcgatggc tgattcgcct acgccccgtt
     1261 tcgatctgct caagctggaa aagtacagcg tcgtgacgct gcagttttcg cgcgggtgcc
     1321 cgtatctttg cgaattttgc gacatcatcg tcatgttcgg aaggaagccg cgctggaaga
     1381 gccccgaaca gatcggcctc gaactcgacg aattgaggaa gcgaaacata cgcgacgcct
     1441 tcttcgtgga cgacaatttc atcggtaacc gcaaggcggc aagagcgctg ttgaaatatc
     1501 tcaaggacta tcaggagcgc caccgctacc gcttcatctt cggaacggag gtgtcgctga
     1561 acgtggcgca ggacgacgaa ctgctgcggt tgttccgcga ggcgaacttc gcctgggtct
     1621 tcatcggcat cgagtctccg gacgtcgaca gcctgaagga gacgaaaaaa tcccagaacc
     1681 tgcaaaacga catactggac tcggtgcgca agatctatgc gcgcgggatc gacgtcctgg
     1741 ccggattcat catcggcttt gataacgaca cgctggggac gttcgaccgg caataccgct
     1801 tcatcgttga ctccggcatt caggcggcga tggtcggcct gctcacggcg ctgccgcgca
     1861 cccccctcta ccagcggctg gagaaagagg ggcgcctcat tgcgcaggcc gagagcacga
     1921 acaatacgcg tcttggcacc aatatcgcgc cgaagcgcat ggaatacgac gccatggttg
     1981 catcgtacaa ggccctctac acgcgcctgc tcgaagaccg gcagatcgcc gatcgggtcc
     2041 ggaacaaggc gcgctatttg cgcgatcccg cccgccagag tcaatacggc ctgatcgagg
     2101 gactgggcat ccttggccgg ttgatggcga ggggaatact ggcgggcggc tggtccagat
     2161 tgtttcattt cctgcgcagc ctgcccctgg cctcgccgcg cttactgccg caggccatca
     2221 ccgactggat catggggctg gccatgcggg actatgttga gcgacggttc ggtgtcgcga
     2281 tgaaaacccg gcaagtcgcc gcggtgaggc tggtcgaatc cctgcaggct gcgctggcgg
     2341 tctacctgcg gagcggcaag gtcatcgtgg cgttgaaggg cggccccgca gccgggctga
     2401 acctgtttgt ctccttgagg ggatggctgg acagggcctt cttctcgcgc gcagccagcc
     2461 accttgagaa gctgctcaag cgtacccccg caaccgtcac tcttcgcatc gaactggcac
     2521 cggaggcgga aatttctcaa ctgaaccggc tgctgcgacg tctggcccct tacggtgacc
     2581 ggatcttcat ccacgtcgat gcgaagctac gcgagcttgt ccggatcgat tcatcggtgt
     2641 tccatttgat cctggagtga cggcctgtct ctcccccttc tgtgaggacg agccgcagtg
     2701 caagctcgtg tgcccgtcca actgcatcct cgagcactcc gacttccgcg agagccgcga
     2761 cgagctgctg gcgaagtatc acagcctgga cggctgagga acctgcggga cgtactcatg
     2821 acccgcaggt tatcgcctgg tcgcaatccg cccgaagcgg tcatttctgc tgaatcttag
     2881 agcccctaac ataatgaaac gcgcgtgcgt tgtgagccag aatcgtcgag agcgcgaagc
     2941 gaggtgcagc caatactcgc tgtattggca agccgagcga caaagctatc ggcgattgtg
     3001 gccgcaaccc cttgtgggct ggtgcgattt tggcctgcgg tgcgtgcgcc gactgacttg
     3061 atattcaact tcccgagcat ctgctgccca tcctctgcca aaatcgtgac agcgcagcgt
     3121 gtgtttcatt atgttagggg ctcttaatct ggtggatcag ttcgaggccg gttggagcgc
     3181 caatctagac tcgaacgatt tgcggtgatg cgccgcaacg gtgacggaag catcctggat
     3241 tattttgctt tgccacgccc agcgcaggat tgcctccgta gggtccgtat tgctgtaatg
     3301 agggccggtg cccacgggaa gacatcaaaa gcgtcattca aagcgataat tttctatcga
     3361 cccgtatttt ctatgagggt aatctcatct tcagcgaaga tcacgccaac gtgcctattg
     3421 cctagcacca cagagtaagt atttttgcca tgcgggcctc gtggaaacgc gtacttgatg
     3481 acgccttcac cgcgaagcgt tcttactttg tctcgaactc gatacgctgt gcgttcaggt
     3541 ttacgtttta cgatcttcat gcgagcggct tacggcggtg taatcgatct aataagcgtt
     3601 tttgccatat gcctcacttg gagtcacgga cgcttgcgat ggaatgtcct acgcgcacct
     3661 cggatcctga atgatcctca ccgggaaggc tctcaatttc tgaacagtcc aaaaaaattc
     3721 ctgggaaagt cagcccgcgg cggcgcggag gcctttcaga tgatgatcga ctcgaccatc
     3781 caaccgattt cactggctct cgcttccttg gatcaggagt tgaacaaaaa gctgtaagtg
     3841 ccgactgtct tgagtggtga gcgaggtgaa ttcgagtcct gacaaatagc ccttctccgt
     3901 ccgttccgtt ttgacgattt tggcgtaaat gtcggttgcc ttgctaccga ccaacggcaa
     3961 gtcgagggcc aacttgagct ccgaggaaga ggtaagttcc tgatcggttt cggcgaggac
     4021 gccatgatag ccgatgttca aagtcgtgcc ctgatgcacg tgcggcaagg cgacatcgtt
     4081 ctcgatcact tgatagaaga acggcatgct gatcgcgacg cgggggcttc tacgaatctc
     4141 gcggc
//
LOCUS       DBNGWZ010000140        10075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3162078, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000140 DBNGWZ010000000
VERSION     DBNGWZ010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3162078"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16CX_04220"
     CDS             complement(<1..794)
                     /locus_tag="AC16CX_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3011752.1"
                     /translation="MPQLNIRRYLKHGILPQLRVFEASARLRCFARAAEELHLAQPTV
                     SVQIKKLSETVGFPLFEQVGKHIYLTDAGQRVYAGCNDVFRALSSLEERLTEMRGLDS
                     GHLQLAVTSTAKYFAPRLLGAFIQRYPEVETSLQIHNRSTLIERLRNNEDDLYLFADP
                     PDQQEVMVQTILSNPLVVLARSDHPLAGEKDIAFAHLANEPFLIREPGSGTRMVTLKL
                     FEQHGLTPKIRMELNTNEAIREAILAGLGVSILSRYTFGLEPEQTKL"
     gene            1294..4716
                     /locus_tag="AC16CX_04225"
     CDS             1294..4716
                     /locus_tag="AC16CX_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024643.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3011753.1"
                     /translation="MHDKESHGKPIGELVSGFQTHLDRGLTLQEAQDRLRKFGANELT
                     ERPRPGFFALLWDQFNNYLVIILIVAALIALALGEWVDSIAIMFIVMLNAVVGVVQES
                     KAEQALAALKKMSAPNAQVIRDGHQITIPGRELVAGDIVLVEAGNYIPADLRLVASVN
                     LKVEEASLTGESVPVEKNAAVVLDKEIPLGDRKNSAFMGTMVTYGRGTGLVTATGMST
                     QIGLIAEMIQSFEDEETPLQHKLEHLGKVLGTICIAICAFVFVYGLIRDTHLTDVFTI
                     GFLNYLQAEQKDIINLFMTAVSLAIAAVPEGLPAIVTICLALGMQQMVKRHALIRKLP
                     AVETLGCATVICSDKTGTLTQNEMTVVQGWTGSKRFRVTGEGYNPNGQFVVDGKPFEP
                     HADPDATLLLHGALLCNDAKLEERSDEAGRRSWQIIGDPTEGAMAVVAMKGGFGRGEL
                     EKALPRIQEIPFDSDRKRMTTIHSLDGANGQASAASFRYPQFVAFVKGAPDIILDLCG
                     QIQEAGQAVPLTEEKRKEVLDQNRDLASNALRVLGVAYRPLKEVPESCTPENIEKELT
                     FVGLLGMIDPARPEVIPAVKVAKGAGLKSVMVTGDHKETAEAIARDIGLLSKGGLVLT
                     GPEIEKLSDEELAAKADKLEVCCRVSPTHKTRIVDAMKARGHVVAMTGDGVNDAPALK
                     RANIGIAMGITGTDVTKQTADMVLTDDNYASIVAAIEQGRIIYSNIRNFVYFLLACNV
                     GEILIIFGSMLFGLPIPLRPVQLLWLNLVSDGAPALALGMEKGDPDIMKHQPRPPKEP
                     VINRDMAIGISVIAVVDAIAILLAFYFGLQRYPGHLEAAQTIAFVTLCASELIRAYTA
                     RSEYHSLFSIGVFSNKWMNWAVLASFLLVLVVVYVPFLRPFFDTVPLTLDDWLLMFPF
                     FFASPIAMELLKVYFRKRTAGTMETAQAPAFGAPVASFAQRSASLASISQQFAGGNTM
                     LKILIPVDDSRNCQFAVKHVIKEFMNNTAMEIHLLNVQPPFSSYIARFVSRKALHDYH
                     RDEAEKVLRPIRQMLDDFGVPYSAHTEVGDRAKSITDTARRLRCDHIVMSTARKNSLT
                     RLVENSVTNKVLELTSVPVEVIAGDAVSRWERYGIPAGLSALLALLLAAAAD"
     gene            4903..5907
                     /locus_tag="AC16CX_04230"
     CDS             4903..5907
                     /locus_tag="AC16CX_04230"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3011754.1"
                     /translation="MARVGVIGGGAFGTAMACVTRRAGHDVVIWAREPEVVAAINNDS
                     ANPVFLKGIRLTPGIRATNDFAAAVAAADFLLLAPPAQHMRAVTGQFRPFLKNGTPVV
                     TCSKGVERKTCALMSQVIAETLPAAPLAVLSGPSFAHDIAVDRPVGVTLACADRALGE
                     RLAQAIGTPRFRTYLSDDVVGALIGGALKNVIAIACGVAIGRKLGESTRATLFARGLA
                     EMARLGVAMGARLETFMGLSGAGDLNLSCNSASSRNTSLGIALGEGRKLQDILRERVT
                     VQEGVHSAESVAVLARRHDVDMPIAVAVDRVLNHGAGVDEAIAQLLAHPYHFDRVNAT
                     "
     gene            6007..7131
                     /locus_tag="AC16CX_04235"
     CDS             6007..7131
                     /locus_tag="AC16CX_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3011755.1"
                     /translation="MIGFIKLLGVALVSLFVTAGPAAAQAIENELVLITPVARTLTDP
                     ALADFAKYAKERWNIDVKTSSLAAGTPVAYGRIVEWKGRPDADIFWGGESALFDKLAE
                     QNLLAKLDLPKGVVDAIPVSIGKPKPIPLKDPKGFWIGTVLEPYGLVYHPKLLARLGV
                     PEPKDWDDLLHPRLKGQVAQCAPTRSSSSHATYEVILQRDGDEKGWEWLKRLAGNTGI
                     FTARSRDVPSVVARGEFAAGFAVPSYMAFEDRLAGYEIKFVAPKTAWITPEPIAIIAG
                     TRHPRAARAFIEYMLSERGQRVAMERGVFPITPKYRVQGKPGSHAEMAVEFTGGLRSY
                     FDVDVVNIYDDDVAQKRYAEVNSQFRKEIEAVAEELKRKY"
     gene            7156..8232
                     /locus_tag="AC16CX_04240"
     CDS             7156..8232
                     /locus_tag="AC16CX_04240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011756.1"
                     /translation="MRISVKNLTKRFGPLAAVSEVSLSIEEGEMFTLLGPSGCGKTTL
                     LRLVAGFYTPDEGEIRFGDRVVNQVPPHERGIGMVFQNYALWPHMTVSENVSYGLKLR
                     KISPSDIAGRLAAVLEKVKLSGLGERYPGQLSGGQQQRVALARALVLNPQILLLDEPL
                     SNLDAKIRVQVRAEIRKLQKELGITTVYVTHDQEEALTLSDRIAVFNQGKVFQIGPPK
                     ALYERPANRFVADFIGINNLVDGTVRAVEGPLQTLRVETAVGELSAIYDKRLSVGDRC
                     VICIRPENAVVDGESGGERNRLKGKITFAAYLGNTLRYDVDLGNGVTFKADIGDPWHH
                     EQLAMGRPVTLSCPVASTLAIPAD"
     gene            8238..9944
                     /locus_tag="AC16CX_04245"
     CDS             8238..9944
                     /locus_tag="AC16CX_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011757.1"
                     /translation="MATASIAAVPARGPRVPVISMIGFALIWAFLILFVLYPLTRIFY
                     DAFTNEAGQFTLANFHEFFTDRYYLRSLWNSLVLGVAAVITTSVIGIAVAFLIIRYDF
                     PYRNLFAYLTMLPMILPPLVGVLGFVFILGRAGTVNVWLMDWLHLAHPINFMYGMQGV
                     LLVETVHLFPLMTLSILDAMSKVDPSLEEAAQGMGARGWRRFWDVTLPLTTPGYVSGA
                     LLVFIWTFADFVTPLVVGVQDLLAVQAYLNIVQFVDRRIFRMGIVISALLVLLAIVFV
                     LVARHYVAIKDYSSLAYSKVERRRLGPVKRWLAVGFLVLLLLVSAIPQVGVVLAAVGK
                     GWALTPFPVHYTFDYFRQVSIETPKFIINSLMFSGLAVLMCLVVGVPMAWIMSRTQTP
                     GRGAMDALTTLILAIPGTAVGIAYIRAFHYPLPGIDLALTSTWFILPLVLAVRRLPYT
                     VRGSYSSLLLVHKSMEEAAENVGATKLKTFRDVTAPLVWKGILVGALFSFIMSIQEAS
                     ATLFLVLGGWEMIPIGIFTYYIAGSHSQAAALGVILIVLCAVSLWVVNRVAGTRVGGL
                     FG"
BASE COUNT         1826 a         3087 c         3204 g         1958 t
ORIGIN      
        1 agctttgtct gttccggttc caacccgaag gtgtagcggg agagaatcga cactccgagt
       61 ccggcaagaa ttgcctcccg gatcgcctcg ttggtgttca gctccattcg aatcttcggg
      121 gtaagaccat gctgctcgaa aagcttcagc gtgaccattc gcgttcccga tcccggttcg
      181 cggatcagga atggctcatt ggcgagatgc gcaaatgcaa tgtccttctc gcccgctaac
      241 ggatggtcac tgcgtgcaag aaccaccagc gggttggaca gaatggtctg gaccatgact
      301 tcctgctggt cgggtgggtc agcgaacagg tacaggtcgt cctcgttgtt tctcagccgt
      361 tcaatcaggg tgctccggtt gtggatctgc agcgatgttt cgacctcggg atagcgctgg
      421 atgaaggccc cgagcagccg aggggcgaaa tacttggccg tcgaagtcac ggctaattga
      481 agatgcccgg aatccagccc gcgcatctcg gtcagtcttt cctcgaggct cgagagggcg
      541 cgaaacacat cattacagcc cgcgtaaacc cgctgacctg cgtccgtcag gtagatgtgc
      601 ttgcctacct gttcaaatag cggaaaacca acagtttcac tcagcttctt gatctgcacc
      661 gaaaccgttg gctgggcgag gtggagttcc tccgcggcgc gagcgaaaca tcgcaatcgc
      721 gcgctcgcct cgaatacgcg aagttgcggc agtattccgt gtttcaggta acgtcgaata
      781 ttcagttgcg gcatggcgat acctcctcga atccgttgcc attgccgcca tgccctaaat
      841 gcacagcggc cttcacctcg aggtgaaggc cgctctcggg aacagacctt cacctattcg
      901 gcaaaggtct cgctcgcatc gtttcctgcg atgcctacgc gaccgggggg gtacccgtct
      961 tgacgaccgc gtagtagcag ccaaccggct gccgaagcta ctcccctctg gaggggcgcc
     1021 tggcttcagc caggccacgc aagactttta gcatatcgaa ccccgcgttt caagtggcct
     1081 gaacattacg aatcgtaatg ccaggcttca atcattgtcc ggggctttcc tctttcgtaa
     1141 tttcaagcat cgcaaacttc aaattttctt tgcacttgtg ccgacccata cttgggcaca
     1201 acgaaacggg caggggccac gcggcaagcc tggcaacgtg gatcgtaaat cggaggcaac
     1261 gccagagacg catgaaatct gaaggagact tgtatgcacg ataaagaatc ccatggcaag
     1321 ccgatcgggg aacttgtcag cggctttcag acacacctcg atcgaggcct gaccctgcaa
     1381 gaggcgcagg accgtctccg gaaattcggc gccaatgaac tgacggagcg cccgcggcct
     1441 ggcttcttcg cgttgctctg ggatcaattc aacaattacc tcgtgatcat cctgatcgtc
     1501 gcggcactca tcgcgctggc gctgggagag tgggtcgact ccatagccat catgttcatc
     1561 gtcatgctga acgccgtggt cggagtcgtt caggaatcga aggcggaaca ggccctcgcg
     1621 gccctgaaga agatgtccgc ccccaatgcc caggtcatcc gtgacggcca tcagatcacg
     1681 atcccgggcc gggagctcgt tgcgggggac atcgttctcg tagaagcagg caactacatt
     1741 cccgccgact tgcgcttggt cgccagcgtc aatctcaagg tcgaagaagc ctcgcttacc
     1801 ggggaatcgg tacccgtgga gaagaacgcc gcagtggtgc tggacaagga aatacccctg
     1861 ggcgaccgca agaactccgc tttcatgggc acgatggtga cgtatggccg cggcacgggc
     1921 ctggtgacgg ctacggggat gagcacgcag atcgggctca ttgcggagat gatccagtcc
     1981 ttcgaggatg aggaaacgcc gctgcagcac aagctggagc atctcgggaa ggtgctcggc
     2041 acgatctgca tcgccatctg cgcgtttgtt tttgtctacg gcctcatccg ggatacgcac
     2101 ctgacggatg tcttcacgat cggtttcctc aactatctgc aggccgagca gaaggacatc
     2161 atcaatctct tcatgaccgc cgtgagtctc gccatcgcgg ccgtccccga aggtctgccg
     2221 gccatcgtga ccatctgtct cgcgctgggg atgcagcaga tggtcaagcg ccatgccctg
     2281 atccggaagc ttcccgccgt ggaaacgctc ggctgcgcca cggtcatttg ttccgacaag
     2341 acggggacgc tgacgcagaa cgagatgacc gtggtccagg ggtggaccgg gtccaagaga
     2401 ttccgggtca cgggggaagg atacaacccg aacgggcaat tcgtcgtgga cgggaagccc
     2461 ttcgaaccgc acgccgatcc ggatgccacg ttgctcttgc acggggcgtt gctttgcaac
     2521 gatgcgaaac tggaggaaag aagcgacgaa gcgggcaggc gctcgtggca gatcattggc
     2581 gatcccaccg agggggccat ggccgtcgtg gcgatgaaag gaggcttcgg gcgaggcgag
     2641 ctggaaaagg ctttgccccg tattcaggag attccgttcg attcggaccg aaagcgcatg
     2701 acgacaatcc acagcctgga tggcgctaac ggtcaagctt cagccgcaag cttccgttac
     2761 ccgcagtttg tcgccttcgt caagggtgct cccgacatca tcctggatct ttgtggccaa
     2821 atccaggagg ccgggcaagc cgttcctctg acggaggaaa agcgcaagga agtgctcgat
     2881 cagaaccgcg acctcgcgag caacgcgctg cgggtgctcg gagtagccta ccgccccctg
     2941 aaagaagttc cggaaagctg cactcccgaa aatatcgaga aggagctcac ttttgtcggg
     3001 ctcctgggca tgatcgaccc ggcgcgtccg gaggtgatac cggcggtgaa agtggcgaag
     3061 ggtgccggcc tgaagagcgt catggtaacc ggcgatcaca aggaaaccgc cgaggcgatc
     3121 gcccgcgata tcggcttgtt gagcaagggt ggtttggtcc tcaccgggcc ggagatcgaa
     3181 aagctgagcg acgaggagtt ggcggccaag gccgacaagc tggaggtctg ttgccgggtt
     3241 tcgcctacgc ataagaccag gattgtcgat gccatgaagg cgcgcggcca cgtggtggcg
     3301 atgacgggtg acggcgtgaa cgacgccccg gccttgaagc gggcgaacat cgggatcgcg
     3361 atgggcatca ccggaaccga cgtgaccaaa cagacagcgg acatggtgct cacggacgac
     3421 aattacgcca gcatcgtcgc ggccatagag caggggcgga tcatctactc caacatccgc
     3481 aatttcgtgt acttcctgct cgcctgcaac gtcggggaga tcctcatcat cttcggctcg
     3541 atgctgttcg ggctgcccat cccgttaagg ccggttcagc tcctgtggtt gaacctggtg
     3601 agcgacggcg ctccggccct ggccctcggg atggagaagg gcgaccccga catcatgaag
     3661 catcagccgc gaccaccgaa ggagccggtc atcaatcgcg atatggcgat cggcatcagc
     3721 gtcattgccg tggttgatgc gatagccata ttgttggcgt tctattttgg cttgcagcgc
     3781 tatccgggcc acctcgaggc ggcccagacc atcgccttcg tgaccctgtg cgcttccgag
     3841 ctcatccggg cctataccgc gcgctccgag tatcacagcc tgttctccat tggcgtgttt
     3901 tcgaacaagt ggatgaattg ggcggtattg gcgtcgttct tgctggtcct ggtggtggta
     3961 tacgtgccgt tcctgaggcc gttcttcgac accgtgccgc tcactctgga cgactggctg
     4021 ctgatgttcc cgtttttctt cgcctcgcca attgccatgg aattgctcaa ggtttacttc
     4081 cgcaaacgga cggcagggac gatggaaaca gcgcaggccc ccgcattcgg tgcacctgtc
     4141 gcgagttttg cgcagcggag cgcaagtctc gcatcgatct cgcaacaatt cgcaggaggg
     4201 aacaccatgc ttaagatact catcccggtc gacgactccc gcaattgcca gttcgcggtg
     4261 aagcatgtca tcaaggaatt catgaacaac acggcgatgg agattcatct gcttaacgtc
     4321 cagccgccgt tcagcagtta tattgcgcgc tttgtgagca gaaaggcgct gcatgactac
     4381 caccgcgacg aggcggaaaa ggttctgcgc ccgatccggc agatgctcga cgatttcggt
     4441 gttccctact cggcgcacac cgaggtggga gacagggcga aatccatcac cgatacggcg
     4501 cgccgcctgc gctgcgacca tattgtgatg agcacggcgc gcaagaactc gctgacccgg
     4561 ttggtcgaga attcggttac caacaaggtg ctcgagctga cctcggtgcc ggtcgaggtg
     4621 atcgcgggcg acgcagtctc gcggtgggag cgctacggca tcccggccgg gctcagtgcc
     4681 ctgttggccc tgttgctggc ggcggctgcc gactgacgca acccggttca tgtggagggg
     4741 tttttgtttg cctgactcga gtgggccggt ctcggacact ctcggtcaga tcgagtctgg
     4801 attactgcgc ttaggcaccg cttaccgctt gctgcttgct tttgtcatga cgtgttagag
     4861 ttcgctcgtt tgccaaacca ggctcgacga ggggagggca aggtggcgcg ggtgggagtg
     4921 atcggcggcg gcgcattcgg taccgcgatg gcgtgcgtga cccgccgtgc cggccacgac
     4981 gtcgtcatct gggcgcgcga gccggaggtc gtcgccgcga tcaataacga ttcggccaat
     5041 ccggttttcc tcaagggaat tcgacttacg ccgggcatcc gcgccacgaa cgatttcgcg
     5101 gcggccgtag cagctgccga tttcctgttg ctggcgcctc cggcgcagca catgcgcgcc
     5161 gtcacgggcc agttccgtcc gtttctcaag aatggcaccc ccgtggtgac ctgctcaaag
     5221 ggtgtcgagc gcaagacctg cgcgctcatg tcgcaggtga tcgccgagac gctgcccgcg
     5281 gcgccgctcg cggtgctgtc gggaccctcg ttcgcgcacg acatcgcggt ggaccggccg
     5341 gtcggcgtca cgctggcctg cgccgaccgt gcgttgggcg agcggctcgc gcaggcaatc
     5401 ggaacgccgc gtttccgcac ctatctttcc gacgacgtcg ttggcgctct catcggaggc
     5461 gcgttgaaga acgtcatcgc cattgcctgc ggtgtcgcaa tcggcaggaa actgggcgag
     5521 agcacgcgcg cgacgctgtt cgcgcgcggt ctggccgaaa tggcgcgtct cggggtcgcc
     5581 atgggcgcgc ggctggagac gttcatgggg ctctcggggg cgggcgacct gaatctcagc
     5641 tgcaacagcg cgagctcgcg caacacctcg ctcggcatcg cactaggcga agggcgcaag
     5701 ctgcaggaca tactccgtga gcgcgtgacc gtccaggaag gcgtgcattc ggccgagtcg
     5761 gtggccgtgc tcgcccggcg tcatgacgtg gacatgccga tcgcggtggc ggttgaccgg
     5821 gtgctcaatc acggcgccgg cgtggacgag gccattgcgc agctgctcgc gcatccttac
     5881 catttcgacc gcgtgaacgc gacgtgaaga aggcgctcgg ggcttgatct gtgtcaaact
     5941 ccgaggggtg cggaggagcg caggatacgg ggcgtggggc cgattgcccc gaaaaaggag
     6001 agtgtcatga tcggattcat caagctgctc ggtgtggcgc tggtatctct gttcgtaacc
     6061 gcagggccag cggcggccca ggcgatcgag aacgagctcg tgctgatcac gccggtcgcc
     6121 agaaccctga ccgatccggc gctggcggat ttcgccaagt acgcgaagga aagatggaac
     6181 atcgacgtga aaaccagctc cctggcggcc ggcacgccgg tggcctatgg ccgcatcgtc
     6241 gagtggaagg gccgccccga cgccgacatc ttctgggggg gcgagagcgc gctcttcgac
     6301 aagctcgccg agcagaatct gctggcgaag ctcgatctgc ccaagggcgt cgttgacgcg
     6361 atcccggtga gtatcggcaa gcccaagcct attccgctga aggatccaaa agggttctgg
     6421 atcggcaccg tgctggagcc ctacggcctg gtgtatcacc cgaagctgct cgcgcgcctc
     6481 ggcgtgccgg agccgaaaga ctgggacgat ctgctgcacc ctaggctgaa gggccaggtc
     6541 gcccaatgcg cgccgacgcg ctcctccagc agccacgcga cctacgaggt gatcctccag
     6601 cgtgacggcg acgagaaggg ttgggagtgg ttgaagcgcc tcgccggcaa taccggcatc
     6661 ttcaccgcgc gcagccgtga cgtgccttcg gtcgtggcgc ggggcgagtt cgcggcgggc
     6721 ttcgcggtgc cgagctacat ggcgttcgaa gaccgtctgg ccggctacga gatcaagttc
     6781 gtcgcgccga agaccgcgtg gatcacgcct gagccgattg ccataatcgc gggcacccgg
     6841 catccccggg ccgcgcgcgc gttcatcgag tacatgctct ccgagcgcgg gcagcgggtg
     6901 gcaatggaac ggggcgtatt cccgatcacg cccaagtacc gggtgcaggg caagcccggc
     6961 tcccacgccg aaatggcggt cgagttcacg ggcggcttgc gctcctactt cgacgtcgac
     7021 gtggtcaata tctacgacga cgacgtcgcc cagaagcgct acgcggaggt caactcgcaa
     7081 ttccgcaagg aaatcgaagc tgtcgcggaa gaactgaaga ggaagtactg agctgtcgac
     7141 cggcggcccg gattaatgcg catttcggtc aagaacctca cgaagcgttt cgggccgctg
     7201 gcagcggtca gcgaggtcag cctctccatc gaggagggtg agatgttcac cctcctcggt
     7261 ccttcggggt gcggcaagac cacgctgctc cggctggtgg ccggtttcta cacccccgac
     7321 gagggggaga tacggtttgg cgaccgcgtg gtcaaccagg tgcccccgca cgagcggggc
     7381 atcggcatgg tgttccagaa ctatgccctg tggccgcaca tgacggtgtc cgagaacgtc
     7441 tcctacggcc tgaagctgcg caagatttca ccgtcggaca tcgccgggcg gctcgcggcg
     7501 gtgctggaga aagtgaagct cagcggcctg ggcgagcgct accccggcca gctctcgggc
     7561 gggcagcagc agcgcgtggc ccttgcccgc gcgctcgtgc tgaatcccca gatcctgctg
     7621 ctcgacgagc cgctctccaa cctcgatgcc aagatccgcg tgcaggtgcg ggcggagatc
     7681 cgcaagctgc agaaggagct cggcatcacc acggtttacg tgacccatga ccaggaggag
     7741 gcgctgacgc tgtcggaccg catcgccgtc ttcaaccagg gcaaggtgtt ccagatcggg
     7801 ccgcccaagg cgctgtacga gcggcccgcc aaccgttttg tcgccgactt catcggcatc
     7861 aacaatctcg tcgacggcac ggtgcgggcg gtcgagggcc cgcttcaaac cttgcgcgtg
     7921 gaaaccgcag tgggcgagct ttccgcgata tacgacaaac ggctgagcgt gggcgaccgg
     7981 tgcgtgatct gcatacgccc cgagaacgcc gtggtggatg gcgagtccgg cggcgaacgc
     8041 aatcgcctca aaggcaagat caccttcgcc gcctatctcg gcaacacgct gcgctacgac
     8101 gttgacctgg ggaacggcgt cacgttcaag gccgacatcg gcgacccgtg gcaccacgag
     8161 cagctcgcga tgggcaggcc ggtcaccctg agctgcccgg ttgccagcac gctggcgatt
     8221 ccggcggatt gatccgcatg gccacggcat cgatcgctgc tgtcccggcc cgggggcccc
     8281 gcgtcccggt gatttccatg atcggcttcg cgctgatctg ggcgtttctc atcctcttcg
     8341 tgctgtaccc gctgacgcgg atcttctacg acgccttcac caacgaggcc ggccagttca
     8401 ccctcgcgaa cttccacgag ttcttcaccg accggtacta cctgcgctcg ctctggaact
     8461 cgctggtgct gggcgtcgcc gcggtcatca cgacctcggt gatcgggatc gcggtcgcgt
     8521 tcctcatcat ccgctacgat tttccctacc ggaatctctt tgcgtatctc acgatgctgc
     8581 cgatgatcct gccgccgctc gtgggcgtgc tcggcttcgt cttcatcctc ggccgtgccg
     8641 gcacggtgaa cgtgtggctg atggactggt tgcacctcgc gcatcccatc aacttcatgt
     8701 acgggatgca aggcgtgctg ctcgtcgaga cggtgcacct gtttcccctg atgacgctct
     8761 ccatcctcga tgcgatgtcc aaggtggatc catcgctcga agaagccgcc caaggcatgg
     8821 gcgcgagagg gtggcggcgg ttctgggacg tcacgctgcc cctgaccaca cccggctacg
     8881 tttccggggc gctgctcgtc ttcatctgga ctttcgccga cttcgtcacg ccgctcgtcg
     8941 tcggcgtgca ggatctcctc gccgtgcagg cctacctcaa catcgtccag ttcgtcgacc
     9001 ggcgcatttt ccgcatgggc atcgtgatct cggcgctgct cgtcctgctc gccatcgtgt
     9061 tcgtgctggt ggcgcgccat tacgtggcga tcaaggacta cagctcgctc gcctattcga
     9121 aggtggagcg ccggcggctg gggccggtca agcgctggct ggccgtgggt tttctcgtgc
     9181 tgcttctgtt ggtgtccgct atcccgcagg tcggcgtggt gctggccgcg gtcggcaagg
     9241 gctgggcgct cacgccgttc ccggtccatt acaccttcga ttatttcagg caagtgagca
     9301 tcgaaacgcc caagttcatc atcaactcgc tgatgttttc cgggctggcc gttctgatgt
     9361 gcctggtggt cggggttccg atggcctgga tcatgagccg gacgcagacg ccggggcgcg
     9421 gcgcgatgga cgcgctcacc acgctgatcc ttgcgattcc gggcaccgcc gtcggcatcg
     9481 cgtacatccg cgcctttcac tacccgcttc cgggcatcga tctcgcgctg accagcacgt
     9541 ggttcattct cccgctggtg ctcgccgtgc ggcggctgcc gtacaccgtg cgcggcagct
     9601 actcgtcgct gctgctcgtg cacaagtcga tggaggaagc ggccgagaac gtcggggcga
     9661 cgaagctcaa gacgttccgg gacgttaccg cgccgctggt gtggaaggga atcctcgtcg
     9721 gcgcgctgtt ctccttcatc atgtcgatcc aggaagcatc cgccacgctt ttcctcgtgc
     9781 tgggcggctg ggagatgatt ccgatcggca tcttcaccta ctacatcgcc ggctcgcaca
     9841 gccaggcagc cgcgctcggc gtgatcctga tcgtcctctg cgccgtgagt ctctgggtgg
     9901 tcaaccgcgt ggcaggcacg cgcgtcggcg ggctgttcgg ctgacggtca cgcctgaagg
     9961 atgaaggcgg aaggatgaag atagaaggct gagattttcc ctcccgtacg gcgggggtca
    10021 tccttcatcc ctcatccttg aattttcggc agggccgaaa attcggaggc gtatt
//
LOCUS       DBNGWZ010000141         5922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2280550, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000141 DBNGWZ010000000
VERSION     DBNGWZ010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2280550"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC16CX_04250"
     CDS             <1..518
                     /locus_tag="AC16CX_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3011758.1"
                     /translation="GGMVALRAALTHPDRVAGLILLDTDAGPETGLRKLKFRAMGAGV
                     RVVGLRPFLPSLARLMFGATTRRQNPTLVSEWMGQFASLHVPSVLRCLEALMQRDSLL
                     GRLDQIGVPALVLVGEEDRSLPPPLSRRIHDRLRHSTFGLIPAAGHLSSLERPAPVTD
                     AILGFLSAHAN"
     gene            complement(1011..1190)
                     /locus_tag="AC16CX_04255"
     CDS             complement(1011..1190)
                     /locus_tag="AC16CX_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011759.1"
                     /translation="MEAILSRTPSRQLESAAADWLRATHKLARALLRVAGDALVNYSR
                     AGLLLKSGSRRIRRL"
     gene            1312..2256
                     /locus_tag="AC16CX_04260"
     CDS             1312..2256
                     /locus_tag="AC16CX_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3011760.1"
                     /translation="MENLTGMAVFARVAEAKSFSGAAQRLGISKSAVSKQVAGLERAL
                     KARLINRTTRRLSLTEVGAAFYEHCARMLAEAEAAELAVSRLYAEPRGVLKVTAPAAF
                     GHLHIAPGIPDFIARHSEVSVQIVMTDRAVDLAEEGFDVAIRMTREQAPNIVARRLAP
                     VRWAVCATPEYLKRHGTPRAPRDLARHNCLFYSSLEASSEWRFTGSAGDTAVRVAGNF
                     TVNNSEALREAVLKGLGIALLPTFTVGGDLREKRLQQVLGEYRAHGTFGNEVYAVYLP
                     TRYLSPKIRAFVDFFVERFAPEPYWDYQGTGINRRESL"
     gene            2514..2930
                     /locus_tag="AC16CX_04265"
     CDS             2514..2930
                     /locus_tag="AC16CX_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3011761.1"
                     /translation="MQALCNLAKNYGPLIGRILIALIFLKSGFEKITGFSAVAGFMGK
                     MGMPAPEFLLVGSIVFEIAGGLMVLLGWHARWGALLLVIFMIPATLIFHNFWAVEAAQ
                     YTNQMNHFMKNVSILGALVFIMGMGSGPLSLTRTRE"
     gene            2963..3916
                     /locus_tag="AC16CX_04270"
     CDS             2963..3916
                     /locus_tag="AC16CX_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3011762.1"
                     /translation="MRTMLVTILVAIGVTNAAYGQQTRLSISTGGTGGVWYPMGGAMA
                     NVLSKYLPGAAVTAEVTGGSVANINLLSSKKSDIAFSMVDAAWDGLHGTGKFKEKVPM
                     RTIAVVHPLVMHVVTAEGRGIDRITDFKGKRISTGSPGSGTEVMALRILEAYGINPDR
                     DVVRERLGVADAANALKDRKIDAFMHAAGVPLPSVTDVAATPGVQIKLVDHADALPAM
                     TKKYGPIYAAGTIPARSYSGQDKDSRTINVWGVLFVHETMDEKLVYDILKTLFDRHKD
                     IVIAHREAANMTLENQAIGATAVPWHPGAVKYFAERGVTVK"
     gene            4046..5236
                     /locus_tag="AC16CX_04275"
     CDS             4046..5236
                     /locus_tag="AC16CX_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR3011763.1"
                     /translation="MMARPEPGARAARIARRMAEIEPFHVMELLARARELEAQGRSII
                     HMEIGEADFPTPEPVIRAGIRALEKGGLFYTPTPGLPQLREAIAGYYRSRYGVEVPPS
                     RILVTTGSSAALMLACAALIDPGDEVLLADPGYPANRHFVRVVEGAPRAVPVDAASSY
                     QLTPEHLESNWGPHTAAALVASPSNPTGTLVSTERLSAMAEFVRGKGGTLIVDEIYHG
                     LVYEGEYTTALAFADDVFVINSFSKYFNMTGWRLGWMVAPEPYVRELDKLAQNLYLAA
                     PTPSQYAALAAFEPETLAILERRAREFKARRDYLVPALRKLGFEIPVTPQGAFYIYAG
                     CGGLTKDSFGFARDLLEHAGVAITPGVDFGSNAPERHVRFAYTNSIERLQEGVSRIAK
                     FLKA"
     gene            complement(5423..>5922)
                     /locus_tag="AC16CX_04280"
     CDS             complement(5423..>5922)
                     /locus_tag="AC16CX_04280"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR3011764.1"
                     /translation="FMLLGMAAGALLLVNLPGDMVMLLLGLLVLVYGSSYAINRGLAM
                     RLPRWSAAPIGLFGGTTSSLFGSGGPVYVVYFAGRGATPEQIRATMPAVFVFTTIARI
                     AVFALAELFTMDVFFAAGLLLPVLALGLWLGNRLHGKLPRDQAARVVGGLLVVSGVSL
                     LLRAL"
BASE COUNT         1084 a         1885 c         1907 g         1046 t
ORIGIN      
        1 tcggcggcat ggtggctctc cgcgcggcac tgactcaccc cgaccgagtt gccgggctga
       61 ttctcctgga cacggacgcc ggtccggaga ccggactccg caagctcaag ttccgcgcca
      121 tgggggcggg tgtccgagtc gtggggctcc gcccgttcct gccctccctc gcacgactca
      181 tgttcggggc caccacccgt cggcagaacc cgactctggt cagcgaatgg atggggcagt
      241 tcgccagtct tcacgtcccg tccgtcctgc gctgcctgga agcgctcatg cagcgggact
      301 cgctgctggg gcgcctggat cagatcggcg tgccggccct cgtgctggtc ggcgaggagg
      361 accgctcgct tcctccaccg ctctctcgcc gcattcacga tcggctacgc cactccacgt
      421 ttggcctgat tcccgctgca ggccacctgt cctcgctcga gcggccagca ccagtcaccg
      481 atgcgattct tggttttctc tcggcccatg ccaactgagc ggtcgttgcc taacaagaaa
      541 atttaaaaga ggcaggctcg atttgtcttg ccaaccagca gcactcaagc gaaagatcga
      601 gcccggcctc ttttacttac gatcctcccc tggcactcag cactcaggac tcagcacttc
      661 gtccagttga agagggggaa atcggggcgc agaataacaa attgcaaacc gacatgtcgg
      721 gcaattatta cgagttaagc gacccggact catcatgtgc gatccattcc acgagccaaa
      781 ggtaacctta gctacattac agcgagaaat taccgcagtg aattcgctcg gtgccggaac
      841 tgttccgagt gcactcaccg cctgtgaaca aacggtagga gtcctgtacg cggcggcaat
      901 tggtcagccg tttccgtatg gactcgcgcg caaggtgcca gcaaaccagc gcgctccgcg
      961 acgtcgcgcc cgcgcctgac gctcggcctt acttacggcc ggcatggcgg ttacagccgg
     1021 cggatgcggc ggctgcccga cttgagcagt aaccccgcgc ggctgtaatt cacgagcgcg
     1081 tcgcccgcca cgcgcagcaa ggcgcgtgca agcttgtgcg tggcgcgaag ccagtcggcc
     1141 gctgccgatt ccagctgccg gcttggcgtt ctcgaaagga ttgcttccat gtctgtcact
     1201 cctggcgggc gttgtttacc cgctcacagt gacaatatcg gactccgcac gcaggttgac
     1261 aatcccccta gatagaatat tattgtttct ttaaagaaac tataggggta ggtggagaac
     1321 cttacgggca tggcggtgtt cgcgcgcgtg gccgaggcga agagcttctc gggggcggcg
     1381 caacggctgg gcatctccaa gtccgcggtg agcaagcagg tggcgggcct cgagcgcgcg
     1441 ctcaaggccc ggctcatcaa ccgcaccacc cggcgcttga gcctcaccga agttggggcg
     1501 gcgttctacg agcactgcgc gcgcatgctc gccgaggcgg aggccgcgga gctggcggta
     1561 tcgcggctct atgcggagcc gcgcggcgtg ctcaaggtga cggcgccggc ggcgttcggc
     1621 catctgcaca tcgcgcccgg cattccggat ttcatcgcgc gccattccga agtaagcgtg
     1681 cagatcgtga tgaccgatcg cgcggtggat cttgccgaag aagggttcga cgtcgcgatc
     1741 cgcatgacgc gtgagcaggc gcccaatatc gtcgcgcgcc ggctcgcgcc ggtacggtgg
     1801 gcggtgtgcg cgacgcccga gtacctgaag cggcacggca cgccgcgcgc gccccgggat
     1861 cttgcgcgcc acaattgcct gttctactcg tcgctggaag cctcctccga atggcgcttc
     1921 accggcagcg cgggagacac cgccgtgcgc gtggcgggca acttcaccgt caacaattcc
     1981 gaggctttgc gcgaagcggt gctcaagggg ctgggcatcg cgttgctgcc gaccttcacc
     2041 gtaggtggcg acctgcgcga gaagcgcctg caacaagtcc tgggcgagta ccgtgcacac
     2101 ggcacctttg gaaacgaggt ctacgcggtc tatcttccga cccgctatct ttcccccaag
     2161 attcgcgcct tcgtggattt ctttgtcgaa cgattcgcgc ccgaacctta ctgggattat
     2221 cagggaacgg gaataaacag gcgtgaatcg ctatagcttg ccaagcaact gcaaggagct
     2281 agcgacggac ggctggaagc gcgcgacgaa gtggttcaag aagcacttgg gataaagcgt
     2341 gatgagtgac gagtgataag tgataaggca cccgcgcttg cgcgggaatt agctcctatt
     2401 acctatcacc cctcactcat tttcaatcgg cggggacggc aacactctcg ccgattgttt
     2461 ttttaacggt ggtggcgcag catatgcgcg gcgcttgaat accgggaggt cccatgcagg
     2521 ctttgtgcaa tctggcgaag aactacggcc cgctgatcgg acgcatcctg atcgcgctga
     2581 tcttcctcaa gtccgggttc gagaagatca ccggattttc ggcggtggcg ggcttcatgg
     2641 ggaaaatggg catgccggcg ccggagttcc tgctggtcgg ctcgatcgtg ttcgagatcg
     2701 ccggcgggct gatggtattg ctcggctggc acgcccgctg gggcgcgctg ctgctcgtga
     2761 tcttcatgat tcccgcgacg ctgatctttc acaatttctg ggcggtcgag gcggcgcagt
     2821 acacgaacca gatgaaccac ttcatgaaga acgtctcgat cctcggcgcg ctcgtgttca
     2881 tcatgggcat gggttcgggg ccgctgagcc tcacgcgcac acgggagtga cccggcgcgc
     2941 ccatacttta cgggaggaaa cgatgaggac gatgctcgtt acgatactgg ttgcgatcgg
     3001 ggtgacgaac gccgcctatg gccagcagac gcggctgtcg atttccacgg ggggcacggg
     3061 cggcgtctgg tacccgatgg gcggggccat ggcgaacgtg ctgtccaagt atctgccggg
     3121 cgcggcggtt accgccgagg ttaccggcgg cagcgtggcc aacatcaacc tgctcagctc
     3181 gaagaagtcc gatatcgcct tctcgatggt ggacgcggcg tgggacggcc tgcacggaac
     3241 cggcaagttc aaggagaaag tgccgatgcg cacgatcgcg gtggtccacc ctctcgtcat
     3301 gcacgtcgtc accgccgagg gccgtggcat cgatcgaatc accgacttca agggcaagcg
     3361 catctccacc ggctcgccgg ggagcgggac ggaggtcatg gccctgcgca tactcgaagc
     3421 gtacgggatc aaccccgaca gggacgtcgt gcgcgagcgg ctgggcgtcg ccgatgcggc
     3481 caatgcgctc aaggaccgca agatcgacgc cttcatgcac gcagccggag tgccgctgcc
     3541 gtctgttacc gatgtcgccg ccacgcccgg tgtccagatc aagctggtcg atcacgcgga
     3601 cgccctcccg gcgatgacga agaaatacgg cccgatctac gcggcgggaa ccattcccgc
     3661 gcgctcctac tccgggcagg acaaggacag ccggaccatc aatgtctggg gcgtgctgtt
     3721 cgtccacgag accatggatg agaagctcgt ctacgatatt ttgaagacgc tgttcgaccg
     3781 gcacaaggac atcgtcatcg cgcaccgcga ggcggccaac atgacgctgg agaaccaggc
     3841 gatcggcgcg accgcggtgc cctggcaccc gggcgcggtg aagtacttcg ccgagcgcgg
     3901 ggtgacggtc aagtagcgcg gaaatggata agggggcgac accccctttg ggatccccca
     3961 aaccagaggc tgttgggagc gggcgcgagg tggagccgat cacactcagg aacgccccgg
     4021 gtcatccctc aacagcctgg tgacgatgat ggcgcgccca gagccggggg cgcgcgccgc
     4081 gcgcatcgcg cggcggatgg cggagatcga gccgttccac gtgatggagc tgctcgcccg
     4141 cgcgcgcgag ctggaggcgc agggccgctc catcatccac atggagatcg gcgaggcgga
     4201 tttcccgacg ccggagccgg tcatccgcgc cggcatccgg gcgctggaga aaggcgggct
     4261 gttctacacg ccgacgcctg gactgccgca gttgcgggag gcgatcgccg gctactatcg
     4321 gtcgcgttac ggcgtcgagg tgcccccctc acgcattctc gtcaccactg gctcctccgc
     4381 tgcgctgatg ctcgcttgcg cggcgctcat cgatccgggc gacgaagtac tgctcgccga
     4441 tcccggttat cccgccaacc gtcatttcgt gcgcgtcgtg gaaggcgcgc cgcgcgcggt
     4501 gccggtggat gccgcaagca gctaccagct tacgcccgaa catctcgaga gtaattgggg
     4561 gccgcacacc gcggcggcgc tcgtcgcctc gccgtcgaat cccaccggca cgctggtgtc
     4621 caccgagcgg ctgtccgcca tggccgagtt cgtccggggc aaaggcggta cgttgatcgt
     4681 ggacgagatc tatcacggcc tggtttacga gggcgaatac acgacggcgc tcgcgttcgc
     4741 ggacgatgtc ttcgtcatca acagcttctc caagtacttc aacatgaccg gctggcggct
     4801 tggctggatg gtggcgccgg agccctatgt gcgcgagctc gacaagctcg cccagaacct
     4861 ctatctcgcc gcgcccacgc cttcgcagta cgccgcgctc gccgcgttcg agccggaaac
     4921 gctggcgata ctggaacggc gcgcgcggga attcaaggcg cggcgcgact acctggtgcc
     4981 ggcgctgcgt aagctcggct tcgaaatccc cgtcacgccg cagggcgcgt tctacatcta
     5041 cgccggctgc ggcgggctga cgaaggacag cttcggcttc gcgcgcgacc tgctggaaca
     5101 cgcgggcgtc gcgatcacgc ctggcgtcga cttcggttcc aacgcgcccg agcgccacgt
     5161 gcgcttcgcg tatacgaact cgatcgagcg gctgcaggag ggcgtgagcc gcatcgcgaa
     5221 attcctaaaa gcttgaaccg ccgagaagga cgagcccaat atttgaaccg cagagcacgc
     5281 agagagaaac aagatcaata tttgaaccgc caggacgcca agagaagcaa gacaattttt
     5341 tgcggttctc tccgtgtctc cgtggttcaa tttcggtctt gatttacctt tgtgtccttg
     5401 gtgtccttcg tggttctgct tcttacagcg cgcgaagcag cagcgaaacc ccgcttacta
     5461 ccagcaaccc gccgacgacc cgcgccgcct ggtcgcgcgg gagctttccg tggaggcggt
     5521 tgcccagcca aagccccagc gccaacaccg gcagcagcag accggcggcg aagaagacgt
     5581 ccatggtaaa gagctccgcc agtgcgaata ccgcgatccg cgcgatcgtg gtgaagacga
     5641 acaccgccgg catggtggcg cggatctgct caggcgtggc gccgcgtccc gcgaaataca
     5701 cgacgtagac cggcccgccg ctgccgaaca gcgaggaagt ggtgccgccg aacagtccga
     5761 tcggtgcggc cgaccagcgc ggcaagcgca tggctaggcc gcggttgatg gcgtagctcg
     5821 agccgtacac caggacgagc aggccgagga gcagcatcac catgtcgccg ggcaggttga
     5881 cgagcaacag tgcgcccgcg gccatcccca gcagcatgaa cg
//
LOCUS       DBNGWZ010000142         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2910503, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000142 DBNGWZ010000000
VERSION     DBNGWZ010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2910503"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..322)
                     /locus_tag="AC16CX_04285"
     CDS             complement(89..322)
                     /locus_tag="AC16CX_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011765.1"
                     /translation="MLEQDLAECQQQARLGTVPWAWPFGFVTSSLVGLDARGHLITVP
                     PYRMETDRFLLEHDLTRSCMRGKGYELVPVEKR"
     gene            952..1287
                     /locus_tag="AC16CX_04290"
     CDS             952..1287
                     /locus_tag="AC16CX_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011766.1"
                     /translation="MLQSDGLVVFVKRAGPACALIELVMQHVARSVPAFRVVSQDDPG
                     FPAKVTRVVDDRELDHSWLNGIEFTPTLIRFEGGRETERVVGWDRDGWRRLTGIRDLG
                     EGLPAFQPG"
     gene            1312..>1640
                     /locus_tag="AC16CX_04295"
     CDS             1312..>1640
                     /locus_tag="AC16CX_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011767.1"
                     /translation="MAERLEAKHRGGTLLAARKIEYGENDDPVEVCFSRGWTDGLPVT
                     PPTDERVIAMLKGTTRRPDEVVGKIPPFLADCTVEKAAINAVMGGCKPEYMPVLLAAL
                     EAALEPV"
BASE COUNT          306 a          499 c          517 g          318 t
ORIGIN      
        1 gcaagcgtgc cgcagccggc gatcagccgc aaggcgcgcc gtcttgctga atctaacggg
       61 tcatccctca tccttaagct tgtgcccgtc accgcttttc gacgggcaca agctcgtagc
      121 ccttgccgcg catgcaggag cgcgtgagat cgtgctcgag cagaaagcga tcggtctcca
      181 tccggtaggg agggaccgtg atgagatgtc cccgcgcatc gagtccgacc aggctcgacg
      241 tgacgaaccc gaacggccag gcccaaggca ccgtccccag ccgcgcctgc tgctggcact
      301 cggcgagatc ttgttcgagc atcgcggtgc cgcttccgga tttctgccag tgcagatcgg
      361 cgcagccggc ggcgattagg acaattaaac tagccgcaga taaacgcaga taaacacgga
      421 tcatatagcc ttgaatcatc gctggcatac tgcgtcatga tcgcgttaaa ttagacggtt
      481 ccaagtcaaa aacattcccg tcgatttcgt gaaacagtca tcatcgatct gcgcggaaaa
      541 tttactttga ctcttgggtt tgccccgccg attgagcagc ggcctttcgt ccggctcgta
      601 tcggcggacc tagagcctat aacaccatca aacacacgtg cgttgctgcc agaattggtg
      661 atgacgcgaa gcgaggtgca gtcaatatgt cgatattggc aagccgagcg acaaagtcag
      721 cgccggttct ggcggcaacc ccttgtgggc tggcgcgagt tttgccccat ggctttgtcg
      781 caagtccctt gcttagtcgg tctaagcggc tggccttgct tcgcgccctg agccaaaatc
      841 gtgccagcgc atcgtgcgtt tgatggtgtt ataggctctt atccggcgca tagcgtccgg
      901 attaagttga ggtagactat gacccgtcct gaagattcct ttattcccgc catgctgcaa
      961 tccgacggtc tggtcgtttt cgtcaagcgc gccggtccgg cctgcgcgtt gatcgagctg
     1021 gtgatgcaac acgtggccag gtccgtaccg gcgtttaggg tggtgtcgca agacgatccc
     1081 gggttcccgg ccaaggtcac acgggtggtg gacgaccgtg aactcgacca ctcctggctg
     1141 aacggcatcg aatttacgcc gacgttgatc cgcttcgaag gcgggcggga gactgagcgc
     1201 gtcgtcggct gggaccgcga cggctggcgg cggctcaccg gcatccgcga tctcggagag
     1261 gggctgccgg cgttccagcc ggggtgaggc tcgcgctccc tcgagcccgg ggtggccgag
     1321 cgactggagg cgaaacaccg cggcggcacg ctgctcgcgg cgcgcaaaat cgagtacggc
     1381 gaaaacgacg accccgtcga ggtctgcttc tcgcgcggct ggaccgatgg attgcccgtc
     1441 acgccgccca ccgacgagcg cgtcatcgcc atgctgaagg gcacgacgcg ccgtcccgac
     1501 gaggtggtcg gaaaaattcc gccgttcctc gccgactgca cggtcgagaa agccgcgatc
     1561 aacgcggtga tggggggctg caagccggag tacatgccgg tgctgctcgc cgcgctggaa
     1621 gcggcgctcg agcccgtgtt
//
LOCUS       DBNGWZ010000143         5218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1020988, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000143 DBNGWZ010000000
VERSION     DBNGWZ010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1020988"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16CX_04300"
     CDS             <1..638
                     /locus_tag="AC16CX_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011768.1"
                     /translation="AGLPALAQTPSPDALNKTREQLRLDQRALEDARKSGDSARIRAA
                     EDRVRASESAVTRERLRADREKQRVDQEKAKASQAELQKDREQARKDRQALEDARKTG
                     DPAKIKAAEDKLKASWKEVREDRAQVAKDRETASRDQSRVERGREWLRERREDMRRGE
                     EARRDDDRRRDDRYAERDDRGGKGRAKGQGKGKSEWAHSEKKGEGWKKREQ"
     gene            835..1482
                     /locus_tag="AC16CX_04305"
     CDS             835..1482
                     /locus_tag="AC16CX_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3011769.1"
                     /translation="MHETSVIVIRHGETEWNREKRMQGTTDTLLSDVGRAQALALGRR
                     LATRDFSALYSSDLSRARDTASAIAERTDREVVTDPRLRERRFGIFEGLTTAEIVARY
                     PEEHARFASRDPDYEVPGGESARAFTGRCLGCLAEIADRHRGAEVVVVTHGLVLDSLY
                     RAAHGLGHGDRRPVPLINASLNLFDYGGGAWRLVLWGDVSHLAPEEVTVYRGSAA"
     gene            1617..2264
                     /locus_tag="AC16CX_04310"
     CDS             1617..2264
                     /locus_tag="AC16CX_04310"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR3011770.1"
                     /translation="MTTSPDTVVLVHGLWVHGIAMELMRRRIGRCGYRALAYSYPSMR
                     LTLTENTERLIRYCRGLAAPRVHFVGHSLGGLVVLRVLESATGFVPGRTVLIGTPFVG
                     SFAARRLAQLPGGRAALGRSFPEWLESARPMLDGRFEIGVIAGKLPIGLGRLVAPGLP
                     GPSDGVVSVAETRLPAMRDHVVLNVSHSGMVLSRVVAHQICAFLRDGAFTHAGDK"
     gene            2275..3396
                     /locus_tag="AC16CX_04315"
     CDS             2275..3396
                     /locus_tag="AC16CX_04315"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3011771.1"
                     /translation="MTKTASIHDSRSTTHVFAARRGRLLAAACLALLMGGCTNIGYYV
                     QSVQGQIDVWSRQRAISEVIQDPGSSEETRKKLALAVHAREFAVRELGLPDNASYQRY
                     ADLGRPYVVWNVFATPEFSMKPVQWCLVFVGCVSYRGYFAKEDAERLAAELAEQGHDV
                     YVGGVPAYSTLGWFADPVLNTMLRYPDQKLARVIFHELAHQVVYVKGDTVFNESFAVA
                     VETEGIKRWLARYGGAQDKTDYERSVMRRQGFTHLVEAYHARLKVLYKSGLAPDAKRA
                     QKKRIFEEMGRDYRKLKASWGGFPGYDQWFTQGLNNARLASVSIYNQAVPAFQALLRR
                     EGGDLQRFYGAVNELARLPLEERNASLKALMPKTTASAQ"
     gene            3439..4068
                     /locus_tag="AC16CX_04320"
     CDS             3439..4068
                     /locus_tag="AC16CX_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3011772.1"
                     /translation="MKLASKSFSNNGRIPAEFAFCAPDPKTHVTLSKNRNPELSWSEL
                     PAGTKSLALICHDYDVPSRPDDVNKEGRTISAKLPRVDFYHWVLVDFDPKSGSIRAGE
                     FSEGVTARGKKGPDGPRNTRQGINDYTMWFASDKDMSGDYFGYDGPCPPWNDEIPHHY
                     VFTLYALDVAKCGVSGKFKGPDVLTAIRGHVLGQAALTGVYSLNPALKV"
     gene            complement(4189..5031)
                     /locus_tag="AC16CX_04325"
     CDS             complement(4189..5031)
                     /locus_tag="AC16CX_04325"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF016278.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HIR3011773.1"
                     /translation="MLSYRHLFHAGNFADVFKHALLSRLLIALGAKDKPYFYLDTHAG
                     TGRYDLAHEWAWKAREFENGIARLWERKDVPAALAPYLDAVRSENPKGRLRFYPGSPL
                     IALKLRRPRDRMVFCELNKTDCAELKTSVGRSRNAAVHLMDGYQALGAFLPPRERRGL
                     VLLDSSFDRSGEFLRIARALKNAYQRWPSGIYAAWYPLMAPGAIRGFARDMEKSGMRK
                     ILRLELRVREPGPDAPIPGCGMLVINPPWTFDAEARPLLDWIQRALAPDGAGGVKVDW
                     LVSE"
BASE COUNT          966 a         1603 c         1784 g          865 t
ORIGIN      
        1 tggccgggct gcccgcactg gctcagaccc cgtcgccgga cgccctcaac aagacccgcg
       61 aacagctcag actggatcaa cgggcattgg aagacgcgcg caaatccggc gactcggcca
      121 ggatccgtgc ggccgaagac cgggtgaggg cgagcgaatc cgccgtcacg cgcgagcggc
      181 tccgggccga tcgggaaaag caaagggtgg accaggaaaa agcgaaagcc agccaggccg
      241 agctgcagaa ggatcgggaa caggcgcgca aggaccgcca ggcgctggaa gatgcccgca
      301 aaacaggcga cccggcgaag atcaaggcgg cggaggacaa attaaaggcg agctggaagg
      361 aggtgcggga ggaccgcgcc caggtcgcga aggatcgcga gaccgcgagc cgggatcaaa
      421 gccgcgtcga gcggggccgc gagtggttgc gcgagcgcag agaagacatg cgccgggggg
      481 aggaagctcg ccgggacgat gaccgccgcc gtgacgaccg ctatgcggag cgggacgatc
      541 gaggcggcaa gggccgggca aagggccaag gcaagggcaa gagcgagtgg gcgcactccg
      601 agaagaaagg ggaggggtgg aagaagcgcg agcagtagcc aggcttacgg ccggcgttca
      661 ttaacgatac ggccgcccgg atttccgggc ggccgttttc tttgcaggac gttacaatga
      721 tggcctgaat agtagagagc gtgaaaggaa cgttgattga tggctgttcg tttatgacga
      781 atcacgaccc ggggatgatt gttttcccct cagacacgat ttccggatcg agccatgcat
      841 gaaaccagtg tgatagtgat tcgtcatggc gagaccgagt ggaaccgcga gaagcgcatg
      901 cagggcacga ccgacacgct gctctcggac gtcggccgcg cccaggcgct ggcgctgggc
      961 cgccggctcg cgacccggga cttcagcgcg ctctacagca gtgatctttc gcgcgcgcgg
     1021 gacacggcga gcgcgattgc cgagcgcacg gaccgtgaag tcgtgaccga cccgcggttg
     1081 cgggagcggc gcttcggcat attcgaggga ctgaccaccg ccgagatcgt ggcgcggtac
     1141 ccggaggagc acgcccgctt tgcgagccgc gacccggatt acgaagttcc cggcggcgag
     1201 agcgcgcgcg cctttactgg acgctgcctc ggatgtcttg cggaaatcgc ggaccggcac
     1261 cgcggcgcgg aagtggtcgt cgtcacgcac ggcctggtgc tcgactcgct ttaccgcgcg
     1321 gcccacggcc tgggccatgg agaccgccgg cccgtgccgc tcatcaacgc gagcctgaac
     1381 ctgttcgatt acggcggcgg cgcctggcgc ctcgtgcttt ggggcgacgt gagtcacctt
     1441 gcgccggaag aggtcacggt gtaccgcggc agcgccgcct gaatccgtga ggagtggagg
     1501 gtgaggggtg aggagcgcat gataacgggt cgcccgtcac gctttatcag tcacgcctta
     1561 tcagtcacgc gtcatcagtc acgcttcacc agtcaccgtc caggcggcgg tcggtcatga
     1621 ccacgagccc ggacacagtg gtgctggtgc acggtctctg ggtgcacgga atcgcgatgg
     1681 agctgatgcg gcggcgcata ggacgctgcg gctatcgcgc gctcgcctat tcctatcctt
     1741 ccatgcgcct gacgctcacc gagaatacgg aacgcctgat acgatattgc cgcggtcttg
     1801 cggcgccacg ggtgcatttc gtcggtcaca gcctgggcgg gctggtcgtt ttgcgcgtgc
     1861 tcgaaagcgc gacgggcttt gtgccggggc gcaccgtgct gatcggcacg cccttcgtcg
     1921 ggagcttcgc ggcgcggcgg ctcgcgcagc tgcctggagg acgcgctgcg ctgggacgaa
     1981 gtttccccga gtggctggaa tcggcgcgcc cgatgctcga cgggcgattc gaaatcggtg
     2041 tcatcgcggg caagctgccg atcggtctcg gacgcctcgt cgcgccgggt cttcccgggc
     2101 cgagcgacgg cgtggtgagc gtcgcggaaa cgcgcttgcc ggcgatgcgc gatcacgtcg
     2161 tgctcaacgt gagccacagc gggatggtat tatcgcgtgt ggtggcgcat cagatatgcg
     2221 ccttcctgcg cgatggtgct tttacgcatg ccggtgataa gtgataggtg atgagtgact
     2281 aagaccgctt cgatccacga ctcacgatcc acgactcacg tttttgcggc gcggcgcggc
     2341 aggctgctcg ccgccgcgtg ccttgcgctg ctgatgggcg gctgcacgaa catcggttat
     2401 tacgtgcagt cggtccaggg gcagatcgat gtctggagtc gccagcgcgc tatcagcgag
     2461 gtcatccagg atcccggctc gtcggaagag acccggaaga agcttgccct ggctgtgcac
     2521 gcaagggaat tcgcggtgcg cgagctgggc cttcccgata acgcgagcta tcaacgctat
     2581 gccgacctcg ggcggccgta cgtcgtatgg aacgtgttcg cgacgccgga gttctcgatg
     2641 aagcccgtgc aatggtgcct ggtgttcgtc ggctgcgtga gctaccgcgg ctactttgcg
     2701 aaggaggacg ccgaacgtct ggccgccgag ctcgcggagc agggtcatga cgtgtacgtc
     2761 ggcggcgtgc ccgcctactc gacgctcggc tggttcgccg acccggtcct caacaccatg
     2821 ctccgttacc cggatcaaaa gctcgcccgg gtcatctttc acgagctggc gcaccaggtg
     2881 gtctacgtga aaggggacac ggtcttcaat gaatcattcg cggtcgcggt cgagacggaa
     2941 ggcatcaagc gctggctcgc gcgttacggc ggcgcgcagg acaagacgga ctacgagcga
     3001 agcgtcatgc gccggcaggg attcacgcat ctggtcgaag cctatcacgc gcgactcaag
     3061 gtgctctaca agagcgggct cgctcccgac gcgaagcgcg cgcaaaaaaa acggatcttc
     3121 gaggagatgg gccgggatta ccgcaagctc aaggccagct ggggcggctt cccgggctat
     3181 gaccagtggt tcacgcaggg gctcaacaac gcgcgtctgg cctcggtctc gatttacaac
     3241 caagcggtgc cggcgttcca ggcactgctc cgccgggagg gcggcgatct gcagcgcttc
     3301 tacggcgcgg tgaacgagct ggcgcgtctt ccgctagagg agcgcaacgc cagcctgaag
     3361 gcgttgatgc cgaagaccac ggcgagcgcg caatgacgac tggggtgtga ttttcccgtc
     3421 cgccaagtgg aggggttcat gaagcttgcc agcaagagct tttccaacaa cggccgcatc
     3481 cccgccgagt tcgctttctg cgcacccgat cccaagaccc atgtcacgct gtcgaaaaac
     3541 cgcaacccgg aactgtcgtg gagcgagctg ccagcgggaa cgaaatcgct cgcgctgatc
     3601 tgccacgact acgatgtgcc gtccagaccc gacgacgtca acaaggaagg ccgcaccatc
     3661 tccgcgaaac tgccgagagt ggacttctac cactgggtgc tggtggactt cgatccgaaa
     3721 tcggggtcaa tcagggccgg cgagttttcc gaaggtgtca ccgcgcgcgg caagaaaggc
     3781 ccggatggcc cgcgcaacac gcgccagggc atcaatgact acacgatgtg gttcgcctcc
     3841 gacaaggaca tgtccggcga ctacttcggt tatgacgggc cgtgcccgcc gtggaacgat
     3901 gagatcccgc accactacgt gttcacgctc tacgcgctcg atgtcgcgaa gtgcggcgtg
     3961 agcggaaagt tcaagggccc ggatgtgctc acggcgattc gcggccacgt gctggggcag
     4021 gcggcgctca ccggcgtcta cagtttgaac ccggcactca aggtttgaac cgccgggaga
     4081 agcaaggcaa taattgaacc gcagagacgc ggagagaaca aaataagaac tagagttcga
     4141 tctagtttat gtgatcgttg tttctctcgg cgtgctcggc ggttcaatct actccgagac
     4201 gagccagtcc accttcacac cgcccgcgcc gtccggcgcc agcgcccgct ggatccagtc
     4261 gaggaggggg cgcgcttcag cgtcaaacgt ccacggcggg ttgatgacca gcatgccgca
     4321 gccgggaatc ggagcgtctg gccccggttc ccgcacccgc agctcgaggc gcaggatttt
     4381 ccgcatgccg gacttttcca tgtcgcgcgc gaagccgcgt atggcgcccg gcgccattag
     4441 tggataccac gccgcgtaga tgccgctcgg ccagcgctgg tacgcgttct tcagcgcccg
     4501 ggcgatgcgc agaaactccc ccgaacgatc gaaggacgag tcgagcagca ccagcccgcg
     4561 ccgctcccgc ggcggcagga atgcccccag tgcctggtaa ccgtccatca ggtgcaccgc
     4621 cgcgttgcgg ctgcggccta ctgacgtttt gagctcggcg cagtcggtct tgttcagctc
     4681 gcagaacacc atgcggtccc ggggccggcg caacttcaac gcgatgagcg gcgagccggg
     4741 atagaagcgc agccttccct tcgggttctc ggaccgcacg gcgtccagat acggcgccag
     4801 tgccgccggc acgtccttgc gctcccacag cctcgcgatg ccgttctcga actcgcgcgc
     4861 cttccacgcc cactcatgcg cgagatcgta gcgcccggtg ccggcatggg tgtcgaggta
     4921 gaagtaaggc ttgtccttgg cgccgagagc gatcagcagc cgcgagagca gcgcgtgctt
     4981 gaatacgtcg gcgaagttgc cggcgtggaa caggtggcga taagacagca tgcttgcggg
     5041 agggcgggag ggcgggagcg ggaaaggaca acaggccgtt tccctcttac gacctcgcgc
     5101 tctcacgacc tcccgggttt tcagagcttg ttgtatttcg tcgacctgcc gcgtgacttg
     5161 gcgatggggt acttccgcgc gttgagcttg agcttgcgct ccgccgccgc ggcgaggt
//
LOCUS       DBNGWZ010000144         5909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2406550, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000144 DBNGWZ010000000
VERSION     DBNGWZ010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2406550"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      309..599
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            697..2661
                     /locus_tag="AC16CX_04330"
     CDS             697..2661
                     /locus_tag="AC16CX_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR3011774.1"
                     /translation="MLCRRGIAAALSAASFTTVAADRPVAVGEPVIVTATRFSDPGER
                     FPIGVQVITQEDIKRSTAATVPELLRQLPGIRTRDNTGSPNVQIDMRGFGIFSDQNTL
                     VLLDGVRVSENEQATVNWSGIPLSAIERIEILRGSGAVLYGGGATGGTINIITRAPGP
                     DQRTALVNGGAGSYGTREAGIEANLGGSYAALRFNASHLDTDNYRDNNRLRQGNALAD
                     IRWTAGRGSLSLKAGADDQSLRLPGALSEAQIAANRRQAATPRDFSDRTGGFLNLDGE
                     LKLGAGEVAANLGYREKNTHAFIIGSTIDSDMSVWSFSPRVKLPHRLGGREHAFIAGL
                     DWDDWDFDNRNVSAFLGTRHPQAFQRNRAFYFQNTTVLGNAWSLALGARRHEVEYGVV
                     DLANPAQADSRKRDLSAYEIAARYRVSAALAAYGKFGYSFRLPNVNDVYNLFTGVVAL
                     LEPQTSHDREIGIDILAGAGRYRAALYHMDLNNELFFDPQAFVNRNLPPTRRYGAEIE
                     GKWRLGRGLDAFANYTHAVSEFRSGSFGGIPIAGNEVPLVPRHAFNIGTGWAFAPRAR
                     ADVVLRYVGEQAFDADETNVFGRKMPAYTVVDLKLAYENRDWLVSAGVKNLFNEKYFS
                     YGVFDPFTPTFFAYPASERSLFVSARYQLK"
     gene            2762..4498
                     /gene="msbA"
                     /locus_tag="AC16CX_04335"
     CDS             2762..4498
                     /gene="msbA"
                     /locus_tag="AC16CX_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid carrier activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HIR3011775.1"
                     /translation="MTNTALYLRLLSHVRPYWRVFALGCLGMAVVAVTEPVLPALMKP
                     LIEGTFIEKDPVLIRWLPLAIVVLFAIRGAAAYVAAYALGWVGNRLVTDLRNAMFGKL
                     LALPARFYDEQPSGGLISKLTFDVTQVTAAATSALTIVFRDTLAIIGLLAYLLWLNWK
                     LTLFALVMAPLIVGVVRVLSVRLRNSSREVQQAMGYITQVIDEAIGGHKVVKLFGGQD
                     YEKRRFDEEANRVRRHLMKQVSAIAASAPIVQLIASVALAIIIYYAMLQSSAEVLSVG
                     EFAAFVTGMLLLTAPLKRVTDINEYLQKGLAAAESMFRLIDEEAEADRGTVAFGRARG
                     EIRFEDVSFSYGDSGRLALDSVNLVIAPGETVALVGASGAGKTTLANLVPRFYHPTRG
                     RVLLDGHDMETLTLASLRANIALVSQDVVLFNDTVAANIAYGMMNGAGEKDIVAAAEA
                     AHAMEFIREMPQGLATLVGENGMRLSGGQRQRLAIARALLKNAPVLVLDEATSALDTE
                     SERHVQAALEALMRGRTTLVIAHRLSTVEKADRIVALDRGRIAETGTHRELLALGGIY
                     DKLYRIQFAPNP"
     gene            4590..5531
                     /locus_tag="AC16CX_04340"
     CDS             4590..5531
                     /locus_tag="AC16CX_04340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3011776.1"
                     /translation="MAYISVVTPVYRAEGCLEELYRRLVAALSGITQDFEIIMVEDCG
                     GDRSWEIINELARRDPRVKGIQFSRNFGQHYGITAGLDHCDGEWVVVMDCDLQDRPEE
                     IPRLHAKAREGYEVVIARRAQSGGPPSRSLTSWLFYKLFSWLADMEYDGQAGNYRMIS
                     RKVVTRLRQMREQLRFFGALLNWMGFPTATIDVQHDGRHEGRSTYTLDKLWQLASETI
                     IAYSDKPLRLAVRLGFLISLFAFCYGVYILWRALVYGSAITGWASLIVSVYFIGGITI
                     AILGILGIYLGKTYDETKKRPLYIVMHTTFDGERKAD"
     gene            5512..>5909
                     /locus_tag="AC16CX_04345"
     CDS             5512..>5909
                     /locus_tag="AC16CX_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011777.1"
                     /translation="MNARLIERTPWDCAALGHDAFELANAGPEAMTQVGAPGHYTVKV
                     DPLASKQLLHTNGFYYCDTLVEPYCEAERLTLLPHPAAGFDHRPALEPLLAVCRGAFQ
                     HGRFHRDFNVERRVADLRYETWLRTLHAAD"
BASE COUNT         1021 a         1941 c         1915 g         1032 t
ORIGIN      
        1 gcttccatga acacactata gtttcaaaaa tcctgttagg tcaactggta acgaccaatt
       61 ttgaatgttg tttggtatca agatcgccgc cggatttcag ggcgttttca agcccctagc
      121 ccccgcccct gttccatccg ccagggcccg cgggcggagt tctcaagagc ctgacaaggc
      181 acgagaatcg ccctcgccga gacggcgggc gcggctgttg cggtgccgcg caaccgggct
      241 tgagccgcca gggagccggc gtttacaatc gccccgtgcg gggggcgcgc tgcccgtcag
      301 tgaatgcctg cccgtactcg ataccgggtg cccgtccgcg ttttctcgcg gcgggtgaaa
      361 cgggaaacag gtgcggcgaa ccgccggttc gcagttgcaa gttggcagtc ggcagttggc
      421 agttcaacct gctgcaaact gcaaactgac cactgacggc tgagcttaag cctgtgctgc
      481 ccccgcagcg gtaagcgagc gtgggtccgc catcacgcca ctgggtgctg acagcgcctg
      541 ggaaggcggc ggatcaagtt ctcgcgagcc cgtagaccgg cccggaccaa tggtggaaaa
      601 gccgcgggaa ggcggcgcgc ccgttgtctg gtacggcctt cctgcatctt ttccttgcgc
      661 tttcttgcat gcggcgggca tgcgtgagga gaaaccatgt tgtgcaggag aggaatcgcc
      721 gcggcgttgt cggccgcatc gttcactacc gttgccgccg accggccggt tgccgtgggt
      781 gagccggtca tcgtaaccgc gacgcgcttc agcgatcccg gcgagcggtt cccgatcggg
      841 gtccaggtca tcacccagga ggacatcaag cgcagcacgg ccgccacagt gcccgagctg
      901 ctgcgccagc tcccgggcat acgcacgcgc gacaacacgg gctcgcccaa cgttcagatc
      961 gacatgcgcg gcttcggtat tttcagcgac cagaatacac tggtgctcct cgacggcgta
     1021 cgcgtgagcg agaacgagca ggccacggtc aactggtcgg gcataccgct gtcggcgatc
     1081 gagcgcatcg aaatcctgcg cggctcgggc gcggtgctct acggcggcgg cgccacgggc
     1141 gggacgatca acatcatcac cagggcgccc gggcccgacc agcgcacggc tcttgtcaac
     1201 ggcggagcag gaagttatgg caccagagag gccggcatcg aggcgaacct cggcggaagc
     1261 tatgctgcat tgagattcaa cgcgagccat ctcgataccg ataactaccg cgacaacaac
     1321 cggctgcgcc agggcaacgc ccttgccgac atccgctgga cggcgggccg tggatcgttg
     1381 tcgctcaagg ccggcgccga cgaccagagc ctcaggctgc ccggcgcgtt gagcgaggcg
     1441 cagatcgccg cgaaccgcag gcaggccgcc acgccccggg acttcagcga ccgcacgggc
     1501 gggtttctca acctcgacgg cgagctcaag ctcggggcgg gcgaggtcgc ggccaacctg
     1561 ggctaccgcg agaagaacac ccatgctttc atcatcggca gcaccataga ctccgacatg
     1621 agcgtctggt cgttctcgcc gcgggtgaag ctcccccacc ggctcggcgg ccgggagcac
     1681 gcattcatcg caggcctgga ttgggacgat tgggatttcg acaaccgcaa cgtctccgct
     1741 ttcctgggta cgcggcaccc gcaggcgttc cagcgcaacc gcgccttcta tttccagaac
     1801 acgacggtat tgggcaatgc ctggagtctc gccctgggcg cacgccggca cgaagtggag
     1861 tatggcgtcg tcgacctggc gaacccggcg caggccgatt cgcgcaaacg cgacctctcc
     1921 gcctacgaga tcgcggcgcg ctaccgcgtg agcgccgcgc ttgccgctta cggcaagttc
     1981 ggctacagct tccgcctgcc caacgtcaac gacgtctaca acctgttcac cggcgtggtg
     2041 gccctgctcg agccccagac ttcgcacgac cgcgagatcg ggatcgacat cctcgccgga
     2101 gcgggccgtt accgcgcggc gctgtatcac atggatctca acaacgagct gttcttcgac
     2161 ccgcaggcct tcgtcaaccg caacctgccg ccaacgcggc gctacggcgc cgagatcgaa
     2221 ggcaaatggc ggctgggcag aggactcgat gccttcgcca actacactca tgcggtgtcg
     2281 gaattccgtt cgggcagctt tggagggatt ccgatcgcag gcaacgaagt tccgctcgtg
     2341 ccccggcatg cgttcaacat cggcaccggc tgggcgttcg cgccgcgcgc gcgcgccgac
     2401 gtcgtgctgc gctatgtggg cgagcaggcg ttcgatgctg atgaaaccaa cgtgttcggc
     2461 cgcaagatgc cggcgtatac cgtggtcgac ctgaaactcg cgtacgagaa ccgtgactgg
     2521 ctggtcagcg ccggcgtgaa gaacctgttc aacgaaaagt atttcagtta cggcgtgttc
     2581 gaccccttca caccgacgtt cttcgcctac ccggcatccg agcgctcgct gttcgtgtcg
     2641 gcgcgatacc agctcaagta gcaccgcgaa gtcacgggga ctgtcacccg aaggaaagcg
     2701 gcggcgccgc aaggcgccgc cgtttctctt ttcggcccgg gtttgttatt gttccgcggt
     2761 catgaccaat accgcgctct acctgcggct gctgtcgcac gtccgtccgt actggcgggt
     2821 gttcgcgctc ggttgcctcg gcatggcggt cgttgcggtg accgagccgg tccttccggc
     2881 gctgatgaag ccgttgatcg agggcacgtt catcgaaaag gatccggtgc tcatccgctg
     2941 gttgccgctg gccattgttg tgttgttcgc gatccgcggc gccgccgcct atgtcgcggc
     3001 ctacgcgctt gggtgggtcg gaaatcggct ggtgaccgac ctgcgcaacg caatgttcgg
     3061 aaagctgctg gcgctccccg cgcggttcta cgacgagcag ccttcggggg ggctcatctc
     3121 caagctcacc ttcgacgtga cgcaggtgac cgccgcggct accagcgcgc tcacgatcgt
     3181 atttcgcgac acgctcgcga tcatcgggct gctcgcgtac ctgctgtggc tcaactggaa
     3241 gctcaccctg ttcgcgctcg tgatggcgcc gctgatcgtc ggcgtggtgc gcgtgttgag
     3301 cgtgcgcctg cgcaattcga gccgggaggt gcagcaggca atgggctaca tcacccaggt
     3361 gatcgacgag gcgatcggcg ggcacaaggt ggtgaagctc ttcggggggc aggattacga
     3421 gaagcggcgc ttcgacgagg aggcgaaccg cgtgcggcgc catctcatga agcaggtctc
     3481 ggcgattgcg gcgagcgccc ccatcgtgca gttgatcgcg tcggtcgcgc tggcgatcat
     3541 catctactac gcgatgctgc aatcgagcgc ggaggtgctc tccgtcggtg aatttgccgc
     3601 gttcgtgacc ggcatgctgc tgctcaccgc gccgctcaaa cgcgtgaccg acatcaacga
     3661 gtacctgcag aaggggctgg ccgccgccga gagcatgttc cggctgatcg acgaggaagc
     3721 cgaagcggac cgcggcacgg tcgctttcgg ccgcgcgcgc ggtgagatcc gattcgagga
     3781 cgtgagcttt tcctacggcg actccggacg actggcgctc gactcggtga atctcgtcat
     3841 cgcgccgggg gaaacggtgg cgctggtcgg cgcctcgggg gccggcaaga cgacgctcgc
     3901 caatctggtg ccgcgcttct atcatccgac gcgcggccgc gtgctgctcg acggccacga
     3961 catggaaacg ttgacgctcg cgagcctgcg ggcgaatatc gcgctggtga gccaggacgt
     4021 ggtgctgttc aacgatacgg tcgccgccaa catcgcctac gggatgatga acggcgccgg
     4081 ggagaaggat atcgtcgccg ccgccgaggc ggcgcacgcg atggagttca tccgcgagat
     4141 gccgcagggg ctcgcgacgc tggtgggcga gaacggcatg aggctctccg gcgggcagcg
     4201 ccagcggctg gcgatcgcgc gggcgctgct caagaacgcg ccggtgctgg tgctcgacga
     4261 ggcgacctcc gcgctcgaca ccgaatcgga gcgccatgtg caggcggcgc tggaggcgct
     4321 gatgcggggg cgcaccaccc tcgtcatcgc gcaccggctc tccacggtgg agaaagccga
     4381 ccgcatcgtg gcgctcgacc gcggccgcat cgccgagacc ggcacccacc gcgagctgtt
     4441 ggcccttggc ggaatctacg acaaactgta ccgcatccag ttcgccccca acccgtgaac
     4501 agtgatcggt gaacagtgaa aagtaaacag caagaactag acgcatttcc gttctccgtt
     4561 ctccgttcac cgttcaccca gcgctagcga tggcctatat ctccgtcgtc acgccggttt
     4621 accgggccga gggctgcctc gaggagctct atcgccggct cgtcgccgca ctctccggca
     4681 tcacgcagga cttcgaaatc atcatggtcg aggactgcgg cggcgaccgc tcctgggaga
     4741 tcatcaacga actggcgcgc agggacccgc gcgtgaaggg catccagttc agccgcaact
     4801 tcggccagca ctacgggatc accgcggggc tcgaccactg cgacggcgag tgggtggtgg
     4861 tgatggactg cgacctgcag gaccggcccg aggaaatccc gcggcttcac gccaaggcgc
     4921 gggagggata cgaggtcgtg atcgcgcgcc gcgcccagag cggtggcccc ccctcccgga
     4981 gcttgacctc atggctgttc tacaagctct tcagctggct tgccgacatg gaatacgacg
     5041 ggcaagccgg caattaccgc atgatttccc gcaaggtggt cacgaggctc cgtcagatgc
     5101 gggagcagct gcgttttttc ggcgcgctgc tcaactggat gggcttcccc actgcgacca
     5161 tcgacgtgca gcacgacggg cgccacgagg ggcgctcgac ctacacgctc gacaagctgt
     5221 ggcagcttgc ctcggagacc atcatcgcct attccgacaa gccgctcagg ctggcggtcc
     5281 gcctcggttt cctgatctcc ctcttcgcgt tctgctatgg cgtctacatc ctgtggcgtg
     5341 cactggtcta cggctcggct atcaccgggt gggcgagcct gatcgtctcg gtctacttca
     5401 tcggcggcat taccatcgcg atcctcggca tcctcggcat ctatctcggc aagacctacg
     5461 acgagaccaa gaagcggccg ctctacatcg tgatgcacac gaccttcgat ggtgaacgca
     5521 aggctgattg agcgcacccc gtgggactgc gccgcgctgg ggcacgatgc tttcgagctt
     5581 gccaacgccg ggccggaggc catgacgcag gtcggcgccc ccggccacta taccgtcaag
     5641 gtcgatccgc tggcgtccaa gcagctcctg cacacgaacg ggttctatta ctgcgacacg
     5701 ctggtcgaac cgtactgcga ggcggagcgg ttgacactgc tgccacaccc cgctgccggc
     5761 ttcgaccatc gcccggccct ggaacccctg ctcgcggtct gccgcggcgc gtttcaacac
     5821 ggccgtttcc atcgcgactt caatgtcgag cgccgagtcg ccgacctgcg ctacgagacc
     5881 tggctcagaa cgctgcacgc ggcggacaa
//
LOCUS       DBNGWZ010000145        13533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1146734, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000145 DBNGWZ010000000
VERSION     DBNGWZ010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1146734"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC16CX_04350"
     CDS             complement(<1..364)
                     /locus_tag="AC16CX_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3011778.1"
                     /translation="MTPSPATTFKTKEEYVAEVLREGILAGRFPRGARLKQAEIAQML
                     NISITPVREAFRILEAEGYVQNITHRGVVVSPFDASATREIMELRVTLESRLAAAAMQ
                     NMTPQDEADLWKLQRDFEK"
     gene            complement(374..1261)
                     /locus_tag="AC16CX_04355"
     CDS             complement(374..1261)
                     /locus_tag="AC16CX_04355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3011779.1"
                     /translation="MSVLIDAASRFLIQGITGNVGRFSARDMQAYGTRVVGGTASGRS
                     AADVEGVAVFPSVQAACAATQADASIAYVPASAAFDHVIEAFEAGIRLVIYPGDELPV
                     QDAIELRAAAKANGATFIGPNTPGVISPGRAKAGFMPSFCYAPGSLGVISRSGSLSYE
                     ACFRLTGADLGQTTVIGIGGDPIKGLTAAEALDLLHADAETRAIVYLGEIGGNDEYAV
                     AKYAARSGAKPVAALIVGRQAPPGKRMGHAGALIGSHADTHPAKMKALERAGVHATGT
                     VSGLVAAAERALAASRQAA"
     gene            complement(1258..2388)
                     /locus_tag="AC16CX_04360"
     CDS             complement(1258..2388)
                     /locus_tag="AC16CX_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3011780.1"
                     /translation="MLLTEHEGKALLASVGVAVPPGVLVRSAGSVGHRKLPYPVAVKA
                     QIASSGRGKAGGVMRADSPVQARTTAKKLLATKFGEETPEAVLVEPWLAIERELYLSV
                     TVDAAADGYVILYSPRGGVDVEQGAMLARCNVGCARNFRAYRLREALAEVEADAAVRE
                     RVIALARRLVLLASSRDCTTVEINPLAKLADGSLIAADAKVVRDDYARFRAADIAGRI
                     DAEHARQPKAIARALAGDLMLVWLDGEVGLISGGAGMTMAAMDLIADAGGRPACFLDC
                     SANPTPQGYRLAFELLDGEPRVKVILVSIFGGLTQMDRVARVMKEIMSERRSRKPVVF
                     RLNGTNFTQANDILGQAGLHNNATIEEAVREAVALARQGGRR"
     gene            2471..3688
                     /locus_tag="AC16CX_04365"
     CDS             2471..3688
                     /locus_tag="AC16CX_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR3011781.1"
                     /translation="MGKAGEGQHLDAAPAEGRITDEAVAAAKKMIGMQLRPEGPYLQD
                     ATPDTIRNFCNGIGDLNPLYRDAEYGRTSRYASIVAHPMFPMAYGWLGRTRWGLPGVH
                     GFYAGNDWELFRHIRPGDRVTAIERVVGVDEKQSRFSGRLVIQYVEAEFANQHDDLVA
                     RVLGWCTRHERQAAREKGKYKEVKKHEYTREELDAIDKATLDEDAHICGRNIRYWEDV
                     NIGDELPAIVRGPLSLMDTMGFLVGCGRGHTHGVLLKGAVKHPGHFFRNPEAGGGVEY
                     TGIGHHRESIAKEVGVPGTYDYGPQRSAWLASLVTNWMGDAGFLKRVRTELRLFNTMG
                     DTTWCKGRVAGKRIVDDHALVDIDIWAENQRGEVTAPNGSATVVLPSKDVKTRVFRDG
                     SQLDLGCARYTPA"
     gene            3751..4740
                     /locus_tag="AC16CX_04370"
     CDS             3751..4740
                     /locus_tag="AC16CX_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011782.1"
                     /translation="MISTRLLMFPVVVVLGAVLCAASAFAQGPAQAYPNKPIRIVVPV
                     APGGGTDFTARLIGQKLTDAWGQPVIVDNRPGAAGNLGVEIAAKAVPDGYTLVMPITS
                     FPINPSLYRKLPFDTVKDFAPVTLVALAPLLLVVNPSLHATSVKELVALAKSRPGELN
                     FANSGSGTTAHLAGELFKRMAGIDIVSVPYKGGGPAVIALLGGQVQIYFSTIPAALSQ
                     VKAGKLRALAVTSRERVPQLPDMPTIAEAALPGFEVVGWFGLFAPAGTPKPVIARLNG
                     EAVKILAMPDVRERFSGQGLIAGGGTPEALASFLKNEIVKWGKLIQEAGIRIE"
     gene            4780..5787
                     /locus_tag="AC16CX_04375"
     CDS             4780..5787
                     /locus_tag="AC16CX_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011783.1"
                     /translation="MMRGRSAMENRETALEKNFRFWCGVMMALAGASAIAQPFPAKPV
                     RFIVPFAPGGASDIVGRATGQKLTEIWGQPVVVDNRAGGSGQIGTEAVARAAPDGYTL
                     LVVEPTFAIAPSLFSKLPYDTLQDFVYIVQIGQGPQVLVVHPSVPVRSIKELLALARA
                     RPGELNFASPGTGTTGHLGMELFKLLGKVNMVHIPYKGAGPAVVDLVGGQVSIGLVSV
                     SSALPNIKAERLRGLAVTSAKRFVAVPELPTIAEAALPGFDTLQWWGLVAPRATPAAV
                     ISKLAADVARLAEIPDVRERMLTLGAEPVSSSPGRFGVFVRSEIEKWGKLVRASGAKV
                     E"
     gene            5920..7065
                     /locus_tag="AC16CX_04380"
     CDS             5920..7065
                     /locus_tag="AC16CX_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR3011784.1"
                     /translation="MDTKSQSNSNPAQSEDGRKIIQRDPNPHIFRPITLRSVTARNRI
                     VVSPMCQYSATDGVPNDWHFQHLASRAVGGAGIVFAEATNVEPRGRITLYCTGLWNDE
                     QRDAFARIARFVKAQGAVAGIQLGHAGRKGSVARPWEGGKGLSPEQGGWQIVAPSPIA
                     FAEGHPVPQEIDEKTIAGTVSLFAAAARRAREAGFDVIEIHGAHGYLISSFLSPITNR
                     RTDRYGGSFENRARFLLEAVDAVRSEWPDDKPLFVRISCTDWIGGGWDLESSVKLAQA
                     LKSGGKVDLIDCSSGGVDPRQKVSPHPGYQAPFAAAVRSRTGIATGAVGMISSPEMAE
                     QIVAEGQADIIVMARALLNDPYWPLHAAKALKTKIPWPKQYERGDIF"
     gene            7168..8433
                     /locus_tag="AC16CX_04385"
     CDS             7168..8433
                     /locus_tag="AC16CX_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3011785.1"
                     /translation="MNKKFAVLGVLILFTLFLFPAGPARAQCKLGVPSIKVGVQGAAS
                     GPHADYGRQIDMGATMAMEEINAAGGILGCKVETRFMDDENRPATGVKNARLLVTDWG
                     AHFTVGTDSSGVAMAMGPVLAELKRIHFFTHAATHRLTEDLVAQKGVKEIVRVSVPVY
                     QDAVVAALIFKDRKDIKRWATIGADYEYGYTSWNLFKETLKKYRPDVEFVGEAWAPFL
                     TLDFSPHVSAVMAQKPDAIFATPWAGEAVMLLRQALIQGVFDNVQVWWQAMGGSVDVL
                     EGISAEVAKDRFKGKLWATARYIHNWPDTADNRAFIERYRKRWARFPNYSAETTYSAL
                     FIIKAAVEKARSLETDKVIEAMKGMQIRNPGGVRVFRAEDHQFIYNVPAGRPMIDPKY
                     PIPVLGDLKVFEAKEYYRHPPFTPVALTK"
     gene            8601..9458
                     /locus_tag="AC16CX_04390"
     CDS             8601..9458
                     /locus_tag="AC16CX_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3011786.1"
                     /translation="MENFAFQGLIGLSLAMYLWLISAGLTIIFGVLRIVNFAHGSLFM
                     VGAYLAYTFYGQMGLPFGVAIALSLIGTGLLGFAMERGFLSRIYDVDEAYQLLLTFGF
                     ILILDDAVKIIWGGVFKIPPIPEFLDGAWPVFGKSFPVYNAFVIAVGLVVAVGLWALF
                     EKTWWGRIVRATASDREMAGAIGVNVPLVFSGVFVFGAMLAALGGAIGTPVRVVAPGI
                     GSSMIIQAFIITVIGGLGNLKGAFVSSLIVGVLSAYGTLFFPVFELFFIFVIMAVVLL
                     IRPQGLFAR"
     gene            9466..10476
                     /locus_tag="AC16CX_04395"
     CDS             9466..10476
                     /locus_tag="AC16CX_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3011787.1"
                     /translation="MTGRLSPRLTILLVLLVLALFLLPWVAGRYPVYLTIQILILAVF
                     SLGFNLLLGYTGLLSFGQAGFFAVGAYGCAKILLAVPNLFLGILGGVALAGVAALLLG
                     ILSVRHTRIYFSMLTLAFGMMIYSIAWKWRDFTGGDDGLVGIPRAPLEIPGLVSVSVA
                     AMDRYYYVVLALSLLAIVAMYRLVRSPLGLTLQAIRDSETRAAFAGVPVRKYRLFAFT
                     IAGLYAGLAGALLPPLENTVTPPIAHWSTSAEPVLATLMGGIHTFTGPIVGAILFFVI
                     KDVVMRFTEYWLICFGIIVVAMVMGFPSGIMSLFENRRRSAAATPDSKRAAGESGISA
                     KT"
     gene            10476..11231
                     /locus_tag="AC16CX_04400"
     CDS             10476..11231
                     /locus_tag="AC16CX_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011788.1"
                     /translation="MELLTIEGAGKRFGDFVAVNGVTLAIPAGRLTAIIGPNGAGKTT
                     LINLATGALFPDSGAMYFKGEEITRLAVHHRVRRGLSRSFQIMNIFARLSVLENVLVP
                     VLSRRGRTGIPFHGMERETQAVSEARQILREIGLPDHEQLPAGALSHGDQRLLELGIA
                     VATAPELCFLDEPSSGLTPTERSTVLELIRKLSSERRTTFIIVEHDMDVVFSLAHWIV
                     VMNRGEVLAEGSPAEIRENRAVREVYLGEEFSA"
     gene            11228..11929
                     /locus_tag="AC16CX_04405"
     CDS             11228..11929
                     /locus_tag="AC16CX_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3011789.1"
                     /translation="MMLAARDLVTGYGLGAVIHGVSFDVEAGEAVCLLGRNGAGKSTT
                     LRSIMGLARPRSGRVEFMGGEITGRQPFEVARLGIGYIPDDRRIFAELSVEENLEIVR
                     QVARRTGHWTMERVYQLFPVLMELRTKHGSGLSGGEQKMLAIGRALMGNPVLLILDEP
                     SEGLSPLMVKALIGAIHQIRQEGVTLLIADQNVKFARRVGSRGYIMEEGHIRYSGTLE
                     ELWAHEEVLRRYLAV"
     gene            12417..>13533
                     /locus_tag="AC16CX_04410"
     CDS             12417..>13533
                     /locus_tag="AC16CX_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3011790.1"
                     /translation="MSATASFEALTPTSFLLRSGRIYADRCAVIDGERRFTYAELLDR
                     CLRLGGALRNMGVPEGGRVAVLASNTHVLLESHYGVPFAAAVLVALNTRLTADNLTFI
                     VAHSGAQVLIYDYEFEGAARDIAAQVGEGLRLVRAGRSDDQYEHLLAGSGPYHRAVAD
                     ERGLLSINYTSGTTGKPKGVMYHHRGAYLQALAMAMETRLDCDSTFLWTLPMFHCNGW
                     CFPWGVTAAGAVHLCLRRFDPGLVWKHLRESNVTHLNGAPTVLVMLAWHPDAARLARA
                     VRVATGGAPPTPAILKRMAELGMDVTHLYGLTETFGPAVICEWRGEWSRLPMDQQAQI
                     KARQGVGNVISQELRVVDAQGKDVPADGRTLGEIALRG"
BASE COUNT         2240 a         4300 c         4555 g         2438 t
ORIGIN      
        1 ccttttcgaa gtcgcgctgc aatttccaga gatcggcttc gtcctgtggc gtcatgttct
       61 gcattgccgc cgccgcaagc cggctttcga gcgtcacgcg cagctccatg atctcacgcg
      121 tggcggatgc atcgaagggc gacaccacca cgccgcggtg cgtgatgttt tgcacgtagc
      181 cctcggcttc gaggatgcga aacgcctcgc gcaccggcgt gatgctgatg ttgagcatct
      241 gcgcgatctc ggcctgcttg aggcgcgccc cccgcggaaa gcgcccggcg aggatgccct
      301 cgcgcagcac ctccgcgacg tattcctcct tggtcttgaa ggtcgtggca ggggaggggg
      361 tcatgtcatc cgctcaggcg gcctggcgcg atgcggcgag cgcgcgctcg gcggcggcga
      421 ccagtccgct gaccgttccc gtggcatgga cccctgcgcg ctcgagcgcc ttcattttcg
      481 ctggatgggt gtccgcgtgc gagccgatca acgcgccggc gtgccccatt ctcttgccgg
      541 gcggcgcctg gcggccgacg atcagcgcgg cgacgggctt cgcgccggag cgcgcggcgt
      601 acttcgccac tgcatactcg tcgttgccgc cgatctcgcc aaggtagacg atggcgcgcg
      661 tctccgcatc cgcgtgcagg agatcgagcg cttcggcggc ggtgaggccc ttgatcgggt
      721 cgccgccgat gccgattacg gtggtctggc cgaggtccgc gccggtcaag cggaaacacg
      781 cttcatacga gagcgagccg ctgcgggata tcacgccgag cgacccgggc gcgtagcaga
      841 acgacggcat gaacccggcc ttggccctgc cgggcgagat gacgcccggt gtgttgggac
      901 cgatgaaggt cgcgccgttc gctttcgctg cggcgcgcag ctcgatcgcg tcctgaaccg
      961 gcagctcgtc gccggggtag atcaccagcc gtatgcccgc ctcgaaggcc tcgatcacgt
     1021 gatcgaacgc ggcgctggcg ggcacatagg cgatcgaagc gtccgcctgc gtcgccgcgc
     1081 aggccgcttg cacgctgggg aacacggcca cgccttcgac atccgccgcg ctgcggcccg
     1141 aggcggtgcc gccgacgaca cgcgtgccgt aggcctgcat gtcgcgcgcc gagaagcggc
     1201 cgacgttccc ggtaatgccc tggatgagga aacgcgaagc ggcgtcgatg agaacgctca
     1261 ccgccgtccc ccctggcgcg cgagcgcgac tgcctcgcgc accgcctctt caatagttgc
     1321 gttgttgtgg aggcccgcct ggccgaggat gtcgtttgct tgcgtgaagt tggtgccgtt
     1381 cagccgaaac accaccggct tgcgcgagcg ccgctcgctc atgatctcct tcatgacgcg
     1441 cgccacccgg tccatctggg tgagcccgcc gaagatcgag acgaggataa ctttcactcg
     1501 gggttcgccg tcaagcagct caaaagctag ccgatagcct tgcggggtgg ggttcgcgct
     1561 gcaatcgaga aagcacgccg gccggccccc ggcgtccgcg ataagatcca tcgctgccat
     1621 ggtcatgccg gcgccccctg agatgaggcc gacctcgccg tcgagccaca ctagcatgag
     1681 gtcgcccgcg agcgcgcgcg cgatcgcttt cggctgccgc gcgtgctctg cgtcaatgcg
     1741 gccagcgatg tccgccgcgc ggaaacgggc gtaatcgtcg cgcaccacct tggcgtcggc
     1801 agcgatgagc gaaccgtccg cgagcttcgc gagcgggttg atctcgacgg tggtgcaatc
     1861 gcggctgctc gcgaggagca cgagccgccg cgcgagcgcg atcacacgct cacgcaccgc
     1921 ggcatcggcc tcgacctccg cgagcgcctc gcgcagccgg taggcgcgga agttccgggc
     1981 gcatccgacg ttgcagcggg caagcatcgc accctgctcg acgtccacgc cgccgcgggg
     2041 agagtagagg attacgtaac cgtccgcggc ggcgtccacg gtaaccgaga gatagagctc
     2101 gcgctcgatc gcaagccacg gctcgacgag caccgcctcc ggcgtctctt ccccaaactt
     2161 cgtcgccagg agctttttag ccgtcgttcg cgcctggacc ggtgaatcgg cgcgcataac
     2221 tcctccggcc ttgccgcgcc cgctggaagc gatctgggcc ttgactgcga cggggtaggg
     2281 aagcttgcga tggccaacgc tgccggccga gcgcacgagg accccgggcg gcacagcgac
     2341 ccccacgctc gcgaggagcg ccttaccttc gtgttccgtg agaagcatga gaatatacga
     2401 tgcattatat ttgtttggtt gtcaaaagcg tgtagaatga accagtcgtg acggtcagcg
     2461 agggcgggtc atgggcaagg cgggcgaagg acaacacctg gatgcggcgc cggccgaggg
     2521 caggatcacc gacgaagcag tggccgccgc gaaaaagatg atcggcatgc agttgcggcc
     2581 cgaggggccg tacctgcagg acgcgacgcc cgacacgatc cgcaacttct gcaacggcat
     2641 tggggacctc aacccgctct accgtgatgc ggagtatggc cgcacgagcc gctatgccag
     2701 catcgtcgcc cacccgatgt ttccgatggc ctacggatgg ctcgggcgca cgcgctgggg
     2761 actgcccggc gtgcatggct tctacgccgg caacgactgg gagctctttc gtcacatccg
     2821 tcccggagac cgcgtgactg cgatcgagcg cgtggtcggg gtggacgaga agcagagccg
     2881 gttttcgggg cggcttgtca tccagtacgt cgaggccgag tttgcgaatc agcacgacga
     2941 tttggtggcg cgggtcctcg gctggtgtac gcgccacgaa cgacaggcgg cgcgcgagaa
     3001 gggcaagtac aaggaagtga agaagcacga atacacacgc gaggagctcg atgcgatcga
     3061 caaggcgacg ctggacgaag acgcgcacat ctgcggccgg aacatccgct actgggagga
     3121 cgtcaatatc ggcgacgagc tgcccgctat cgtgcgcgga ccgctctcgc tcatggacac
     3181 catgggattt ctggtcgggt gcggacgcgg gcacactcat ggcgtgctgc tgaagggcgc
     3241 ggtcaagcat ccggggcatt tcttccgtaa tcccgaggcg ggtggcggcg tggagtacac
     3301 cggcatcggg caccatcgcg aatcgatcgc gaaggaagtt ggagtgcccg gaacgtacga
     3361 ctacggcccg cagcgctccg cttggctcgc gagcctggtc accaactgga tgggcgatgc
     3421 cgggttcctg aagcgcgtgc ggaccgaact gcggctgttc aacaccatgg gcgacaccac
     3481 ctggtgcaag ggcagggtcg ccggcaagcg catcgtggac gaccacgcgc tggttgacat
     3541 cgacatctgg gcggagaacc agcgcgggga ggtgacggcg ccgaacggga gcgctacggt
     3601 agttctgccg tcgaaggacg tcaagacgcg ggtgttccgc gacggatcgc agctcgacct
     3661 cggctgtgcg cgttacacgc ccgcctgatt cgctgctgca gccgctggcg ctgcggctca
     3721 tcggatcatg agacaaagag gagaattgag atgatctcca cgcgacttct gatgttccct
     3781 gtcgtcgtcg tcctgggcgc ggtcctgtgc gcagcgagcg cctttgcgca gggaccggcg
     3841 caggcctatc ccaacaagcc gatccgcatc gtggttccgg tcgcgcccgg cggcggcacc
     3901 gatttcaccg cgcgcttgat cgggcagaag ctcaccgatg cgtggggcca gccggtgatc
     3961 gtggacaatc gtccaggtgc ggcgggcaat ctcggcgtgg agattgccgc caaggctgtg
     4021 ccggacggct acaccctggt catgccgatc acctcgtttc cgatcaatcc cagcctctat
     4081 cgcaagcttc ccttcgacac cgtgaaggac ttcgcgcccg tgaccctggt cgcgctcgcg
     4141 ccgctgctgc ttgtggtgaa tccatccctg cacgcgactt ccgtgaagga gctcgtggcg
     4201 ctcgccaaat cgcggccggg cgagcttaat ttcgccaact cgggcagcgg caccaccgcg
     4261 catctcgccg gggaactgtt caagcgcatg gctggcatcg acatcgtgag cgtgccctac
     4321 aaaggcggcg ggcccgcggt gatcgcgctg ctcggaggcc aggtccagat ctacttctcg
     4381 acgattccgg ctgcgctatc ccaggtgaag gcgggaaagc tgcgcgcgct cgcggtgacg
     4441 agcagggagc gcgtgccgca gctccccgac atgccgacta ttgcggaagc ggcgctcccc
     4501 ggcttcgagg tcgtaggctg gttcgggctt ttcgcgccgg caggaacgcc aaagccggtg
     4561 atcgcgcggc taaacgggga agcggtgaaa atcctcgcca tgcccgacgt gcgcgagcgg
     4621 ttctcggggc agggcctcat tgccggcggc gggacgccgg aagcgctcgc gagcttcctc
     4681 aagaacgaaa tcgtgaaatg ggggaagctc atccaggaag cgggtatccg catcgagtag
     4741 aatcccggcc tgttgaagta agggagcacc cgccccgcca tgatgcgcgg ccgcagcgcg
     4801 atggagaaca gggagacggc gttggaaaag aatttccgtt tctggtgcgg cgtcatgatg
     4861 gcgctcgcgg gggcgagcgc cattgcgcag ccgtttccgg caaaacccgt ccgtttcata
     4921 gtgccgttcg ccccgggtgg ggcgagcgac atcgtggggc gcgccacggg gcagaagctc
     4981 actgagatct ggggccagcc ggtcgtggtg gacaaccgtg cgggcggctc gggccagatc
     5041 ggcaccgaag cggtcgcgcg cgcggcgccc gacggttata cgctgctcgt ggtcgagccg
     5101 accttcgcga tcgcgccaag cttgttttcg aagcttccgt acgacacgtt gcaggacttc
     5161 gtgtacatcg tccagatcgg ccagggtccg caggtgctgg tcgtgcaccc ctcggttccc
     5221 gtgaggagta tcaaggaact gcttgcgctt gcgcgggcgc gaccgggcga gctcaacttc
     5281 gcctcacccg gcaccggcac gaccgggcac ctggggatgg agctgttcaa gctgctgggc
     5341 aaagtcaaca tggtgcacat cccgtacaag ggcgcgggtc cggcggtggt ggacctcgtc
     5401 ggcgggcagg tgagcatagg cctggtgagc gtgtcatcgg ccctgccgaa catcaaggcg
     5461 gaacgcctgc gcgggctcgc ggtgacgagc gcgaagcgct ttgtcgcggt gcccgagctg
     5521 cctacgatcg ccgaggccgc gcttccgggt ttcgacacgc tacagtggtg gggtctggtc
     5581 gcgccccgcg cgacgcctgc cgccgtcatc agcaagcttg ccgcggacgt cgcgcggctt
     5641 gccgaaatac cggacgtccg ggagcggatg ctgacgctcg gcgccgaacc ggtgtcgagc
     5701 tcccccgggc gtttcggcgt cttcgtccgc agcgagatcg agaagtgggg caagctcgtg
     5761 cgcgcttccg gcgccaaggt cgagtaaacc gtgacatgat gcggcgtgat tttggggtta
     5821 aacgagcggt tttccacgca gcaccacgtc acgtattatc atgtcacgct tcaacacgtc
     5881 acgccccacc atgtcacgct tttccaatca agtgaaatga tggatacgaa atcgcagtcc
     5941 aattcgaacc cggctcaaag cgaggacgga cgcaagatca tccagcgcga tcccaacccg
     6001 catatcttca ggccgatcac cttgcgctcg gtcacggcgc gcaaccgcat cgtggtctcg
     6061 ccgatgtgcc agtactcagc gaccgatggc gtgccgaacg actggcattt ccagcatctc
     6121 gcgtcgcgcg ccgtgggcgg ggcgggcatc gtgttcgcgg aagcgacgaa cgtcgagccg
     6181 cgcgggcgca tcaccctgta ctgcaccggc ttgtggaacg acgagcagcg cgacgctttc
     6241 gcgcgcatcg cccgcttcgt caaggcgcag ggtgcggtcg cgggaatcca gctggggcat
     6301 gccggccgca agggctcggt ggcgcgtccc tgggaaggcg gcaaggggct cagcccggag
     6361 cagggtggat ggcagatcgt cgcgccctcg ccgatcgcgt tcgcggaggg gcatcccgtg
     6421 ccgcaggaaa tagacgagaa gacaatagcc ggcaccgtgt cgctgtttgc cgccgccgcg
     6481 cgccgcgccc gcgaggccgg cttcgatgtc atcgagatcc acggggcgca cggctacctg
     6541 atctcgtcgt tcctttctcc catcaccaac cgccgcaccg atcgttacgg cggcagcttc
     6601 gagaaccgcg cccgtttcct gctggaggcc gtggacgccg tgcgctcgga atggccggac
     6661 gacaagccgc tgttcgtgcg catctcgtgc accgactgga tagggggcgg ctgggatctc
     6721 gagagcagcg tgaagctggc acaggcgctg aagtccggcg gaaaggtgga cctgatcgat
     6781 tgttccagcg gcggcgtgga tcctcgccag aaggtcagcc cccatcccgg ataccaggcg
     6841 ccgtttgccg ctgcggtgcg cagtcggacc ggcatcgcca ccggcgccgt gggcatgatc
     6901 tcgagcccgg aaatggcgga gcaaatcgtc gcagagggtc aggctgatat catcgtcatg
     6961 gcgcgcgcgt tactgaacga cccgtattgg ccgctgcacg cggcgaaggc gctgaagacg
     7021 aagattccct ggccgaagca atacgagcgc ggcgatattt tctgacatca agtttgacgc
     7081 tgcattcgat cggcatatac ttgcagctgc caaggatttg ttcgtccccg ggttttttcg
     7141 aaccttggtt cgtccaaagg aggatctatg aataagaaat tcgcagttct gggcgtcctc
     7201 attctattta ctctgttctt gtttcccgcc ggccccgcgc gcgcgcagtg caagctgggc
     7261 gtgccttcca tcaaggtcgg cgtgcaggga gcggcgtcgg gcccgcacgc ggactacggc
     7321 cgccagatcg atatgggggc caccatggcc atggaggaga tcaacgcggc cggcggcatt
     7381 ctcggctgca aggtcgagac ccggttcatg gacgacgaga accggccggc caccggggtc
     7441 aagaatgccc ggttgctggt gacggactgg ggcgcccact tcacggtcgg gaccgactcc
     7501 agcggcgtcg ccatggcgat gggaccagtg cttgcggaac tgaagcggat ccatttcttc
     7561 acccatgcgg cgacccaccg gcttacggag gacctggtcg cgcagaaagg cgtcaaggag
     7621 atcgtccggg tcagcgttcc cgtctaccag gacgcggtag tcgccgccct gatcttcaag
     7681 gaccggaagg acatcaagcg gtgggcgacc atcggcgcgg attacgagta cggctacacc
     7741 tcctggaacc tcttcaagga aacgttgaag aaataccggc ctgacgtgga attcgtgggc
     7801 gaggcctggg cgccgttcct gacgctggac ttttctcccc acgtctcggc cgtgatggcg
     7861 cagaagcccg atgcgatatt cgcaacgccg tgggcgggag aggccgtcat gctgctgcgg
     7921 caggcgctga tccagggcgt cttcgacaac gtgcaggtct ggtggcaggc gatgggaggc
     7981 tcggtggacg tgctggaggg gatctccgcg gaggtcgcga aggaccgctt caagggcaag
     8041 ctctgggcga ccgcgcgcta tatccacaac tggcccgata ccgcggacaa cagggctttc
     8101 atcgaacgct accggaaacg gtgggcgcgg ttcccgaatt actccgcaga gaccacctat
     8161 tccgccttgt tcatcatcaa ggccgccgtg gaaaaggccc ggagtctgga aaccgacaag
     8221 gtcatcgagg ccatgaaggg aatgcagatc aggaatcccg gcggcgtccg ggtgttcagg
     8281 gccgaggacc atcaattcat ctacaacgtt ccggccggac gcccgatgat tgaccctaaa
     8341 tacccgatcc cggtgctcgg tgacttgaag gtattcgaag ccaaggaata ctatcggcat
     8401 cctcccttca cgccggtcgc attgaccaag taatcggtcc ggcaagaaag ggccccggat
     8461 ccccgctccg gaaatggaag ggcgccattt ccgcagtact tttcccctcg cccctcggga
     8521 gagggggaag gggtgagggg gccgggcgtg ttcgagcgat tgcttaacgc ccggtagccg
     8581 ggccctttct cttgcgcgct atggagaatt tcgccttcca gggtctcatc ggcctgtccc
     8641 tggcgatgta tctgtggctg atctcggccg ggctcacgat catcttcggg gtgctccgga
     8701 tcgtcaattt cgcccacggg agcctgttca tggtgggggc ctatctggcc tacacctttt
     8761 acggccagat gggactcccg ttcggggtgg cgattgcctt gagccttatc ggaaccggac
     8821 tgctcgggtt cgcaatggaa cgcggattcc tgagccggat ttacgacgtt gacgaggcct
     8881 accagctcct gctgaccttt ggcttcatcc tgatcctcga cgacgcggtc aagatcatct
     8941 ggggcggggt attcaagatc ccgccgatac ccgagttcct cgacggggcg tggccggtgt
     9001 tcggcaagtc gttccccgtc tataacgcgt tcgtgattgc ggtagggctg gtcgtcgccg
     9061 tggggctgtg ggcgctcttc gagaagacgt ggtggggacg gatcgtgcgc gcgactgcct
     9121 cggaccgcga aatggccggc gccatcgggg tgaacgtgcc gctggttttt tccggggtat
     9181 tcgtcttcgg cgcgatgctg gccgccctgg gtggggctat cgggacgccg gtgcgcgtcg
     9241 tcgcgcccgg catcggatcg tcgatgatca tccaggcctt catcatcacc gtcatcggcg
     9301 gcctcgggaa tctcaagggt gcctttgtga gctcgctcat cgtcggcgtg ctgagcgcat
     9361 acggaacgct gttctttccc gtgttcgagc tgttcttcat cttcgtgatc atggccgtcg
     9421 tcctgctcat ccgaccgcag ggcctgtttg cgcgctgagc cgcccgtgac tggccgcctt
     9481 tccccgcgtc tcacgatcct gctcgtcctg ctggtgctcg cgctgttcct gctgccgtgg
     9541 gtcgccggac gctacccggt gtacctcacg atacagatcc tgatcctggc ggtgttttca
     9601 ctcggtttca atctgctgtt gggctatacc gggctgctct cgttcggcca ggccgggttt
     9661 ttcgcggtgg gcgcgtacgg ctgcgccaag atcctgctgg cggtgcccaa cctctttctc
     9721 ggaatcctgg ggggcgtggc gctggccggg gtagcggcgt tgctgctcgg aatcctctcc
     9781 gtccggcaca cgcgcattta cttttcgatg ctgacgctcg ccttcggcat gatgatctac
     9841 tccatcgcct ggaagtggcg ggacttcact ggcggcgacg acgggctggt cggcattccc
     9901 cgcgcgccgc tggagatccc cggcctcgtg agcgtcagcg tggcggccat ggaccgctac
     9961 tactacgtgg tgctggcgct ctcgctcctc gcgatcgtgg cgatgtatcg actggtccgg
    10021 tcgcctctgg ggctcacgct gcaagccatc cgcgacagtg agacccgtgc ggcgttcgcc
    10081 ggagtgccgg tccggaagta tcgcctcttc gcgttcacca ttgccggtct ttatgccggg
    10141 ctcgcgggag cgctgctgcc gccgctggaa aataccgtga cgcctcccat cgcgcactgg
    10201 agcacttcgg ctgagccggt cctggcgacc ctcatgggcg gcatccacac gttcaccggc
    10261 cccatcgtcg gcgccattct ctttttcgtg atcaaggatg tcgtcatgcg cttcaccgag
    10321 tactggctca tctgcttcgg catcatcgtg gtggcgatgg tgatgggctt cccgagcggc
    10381 atcatgagcc tctttgaaaa ccgcaggcgc tccgcggcgg caactccgga ctccaagcgc
    10441 gcggcagggg aaagcggcat ctccgcgaaa acgtgatgga actgctcacg atcgaaggtg
    10501 ccggaaagcg cttcggggac ttcgtggcgg tcaacggagt caccctggcg atacccgcgg
    10561 gacggctgac tgccatcatt ggcccgaacg gcgccggcaa gacgacgctc atcaatcttg
    10621 ccaccggggc gctctttccc gactccgggg cgatgtactt caagggcgaa gagatcacgc
    10681 gcctcgccgt gcatcaccgg gtccgcaggg ggttgtcccg atcgttccag atcatgaata
    10741 tctttgcacg cttgtcggtg ctcgagaatg tccttgtgcc ggtgctttcc cggcgcggac
    10801 gcaccggcat accgtttcac gggatggagc gggaaacgca ggccgtgtcg gaggcacggc
    10861 agatcctgcg cgagatcggc cttcccgacc acgagcagtt gccggccggc gcgctctcgc
    10921 acggagacca gcggctgctg gaactgggaa ttgcggtcgc caccgcgccc gagctctgtt
    10981 tccttgacga gccttcgtcc gggctgacgc ccaccgaacg ctcgacggtg ctggagctga
    11041 tccggaagct gtcctccgag cggcgcacga cattcatcat tgttgagcac gacatggacg
    11101 tcgtgttttc gctcgcgcac tggatcgtgg tcatgaaccg tggcgaggtg ctggccgaag
    11161 gatcgcccgc cgagatccgg gagaaccgcg ccgtgcgcga ggtctatctc ggcgaggagt
    11221 tttcggcatg atgctcgcgg cgcgcgacct ggtgaccggg tacggcctgg gcgcggtgat
    11281 tcacggcgtg tcgtttgacg tcgaggcggg agaggcggtc tgcctgctcg gacgaaacgg
    11341 cgccggcaag agcacgacgt tgcgcagcat catgggcctg gcgcggccgc gcagcgggcg
    11401 ggtcgaattc atgggagggg agatcaccgg acggcaaccg ttcgaggtcg cccggctcgg
    11461 gatcggttac attccggatg accggcgcat tttcgcggag ctttcagtcg aggagaatct
    11521 cgaaatcgtc cggcaggtgg cccgccgcac cggacactgg acgatggagc gcgtctacca
    11581 gctatttccg gttctcatgg agttgcggac gaagcacgga tccggcctct cgggcggaga
    11641 gcagaaaatg ctcgccatcg gacgcgcctt gatgggtaat cccgtccttc tcatcctgga
    11701 cgagccctcg gagggactga gcccgctcat ggtcaaggcg ctgatcgggg ccattcacca
    11761 gatccggcag gagggcgtca cgctcctgat tgccgaccag aacgtcaagt tcgcgcgccg
    11821 cgtcggtagc cgcgggtaca tcatggaaga ggggcacatc cgatattcgg ggaccctgga
    11881 agaactgtgg gcgcacgagg aagtgctgcg gcgctacctg gcggtgtgac cagagcctat
    11941 ctcaatagta ggcacgtgtg cgacggaggc gatgtgggat gcagttttag gaggagaatc
    12001 cgcagcaata tgtcgatatt gcaagggttc ccgacaacaa aatgcacccc tgagcggtcg
    12061 cttcgcgacc gcgggcattc cctgccccaa tgggcgcgca acggccgaag gccgttgctc
    12121 agcgccccgt ccctccgggt tgcggccagg acggcacgcg gagggtggag cagtgcctgc
    12181 gcccgtgggc gtaggtgcga ccccgcagcg ggccaagcgc gccgagttga atccgcgtgc
    12241 ctgtgcgcgc ctttgcggtc gcatccccgc aagcagggcc cctgctcaac gctccggcgc
    12301 gccgtctgct ttgttgcccg gcttgaccct atcgacatag gctctgcgcc tcgcgcctcg
    12361 catccatccc gtcctggcca caacgcatca cgcgcctact attgagatag gctctaatga
    12421 gcgcgaccgc gtccttcgag gccctgaccc cgacgtcctt cctgttgcga tcgggccgga
    12481 tctacgccga ccgctgcgca gtgatcgacg gcgagcgccg cttcacctac gccgaattac
    12541 tcgaccgctg cctgcggctt ggcggcgcgt tgcgcaacat gggcgttccc gaaggcgggc
    12601 gcgtcgcggt gctcgcgtcc aacacccacg tgctgctcga atcgcactat ggcgtgccgt
    12661 tcgccgccgc ggtgctggtg gcgctcaaca cccggctcac ggcggacaat ctcactttca
    12721 tcgtcgcgca cagcggcgcg caggtgctga tctacgatta cgagttcgag ggcgccgccc
    12781 gtgacatcgc tgcgcaggtg ggcgaaggat tgcggctggt gcgcgccggg cgttccgacg
    12841 accagtatga gcatttgctg gccggttccg gcccctacca ccgcgcggtg gccgatgagc
    12901 gcggcctgct ctcgatcaat tacacgagcg gcaccaccgg caagccgaag ggtgtcatgt
    12961 atcaccatcg cggcgcctac ctgcaggcgc tggcgatggc gatggaaacg cggctcgact
    13021 gcgacagcac ctttctctgg acgctgccga tgtttcactg caacggctgg tgttttccgt
    13081 ggggcgtgac cgccgcgggg gcggtgcatc tctgcctgcg cagattcgat ccaggcttgg
    13141 tatggaaaca cctgcgcgag tcgaacgtca cgcacctgaa cggcgcgcct accgttctcg
    13201 tcatgctggc gtggcatccg gacgcggcca ggctcgcgcg ggccgtgcgc gtcgcgaccg
    13261 gcggcgcgcc gccgacgccg gcgatcctga agcgcatggc cgagctcggc atggacgtca
    13321 cccatcttta cggtcttacc gaaactttcg gtcccgcggt gatctgcgaa tggcgtggcg
    13381 agtggagccg gctcccgatg gatcagcagg cgcagatcaa ggcgcgccag ggcgtgggca
    13441 acgtgatctc gcaggaactg cgggtagtcg acgcgcaggg caaggacgtg ccggcggacg
    13501 ggcgcacgct tggcgaaatt gcgctgcgcg gca
//
LOCUS       DBNGWZ010000146         3290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_13808, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000146 DBNGWZ010000000
VERSION     DBNGWZ010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_13808"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="AC16CX_04415"
     CDS             complement(<1..1472)
                     /locus_tag="AC16CX_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3011791.1"
                     /translation="MINTTTGARALVDALVREGIDHVFGIPGTQNLAVIDALRDTPRI
                     RFILTRHEQGAAFMAYGFARASGRPAVVTATEGPGVTNLVTAIGAAYRGFVPVISVCG
                     MQESAMRERDASQDMDQVPFMRPITKWAYSIPNVHKVQESVRKTFRVALAEPQGPAHI
                     EASSEILLEKTVPEPIAPAAYRNTVPPACDPAQLDRAMELLALAERPVFVVGRGVLNE
                     AATGTMAQLAERTGVPVATLQYSPDAFPSTHPLALGPLGRNGAVSANRVAPQADLIIA
                     FGAHLDYFSTMYKYGIFSQSAKFIHHSAAPGQIGIVFPVTLGVTGSTASFIAGLVERA
                     GKSGPRKPWVDVAKARADWDAELQAAVRSDAVPIQSQFVAHVIRKVLPRDGLLVVDAG
                     NGGKHVRSYFRSYEPGTFMGIDDWGSVGGSFPIALGAKLARADRPVLCAAGDMGMMCN
                     IGELETAMREKIPVVCVVFNDQGLGNERAFQNEHYGGRFYA"
     gene            complement(1620..>3290)
                     /gene="polX"
                     /locus_tag="AC16CX_04420"
     CDS             complement(1620..>3290)
                     /gene="polX"
                     /locus_tag="AC16CX_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3011792.1"
                     /translation="IKGDNPFRIRAYRNAARTVNELGRDLATLIGERKELPKLPGIGA
                     DLAAKIEEICGSGTCALLERLHRELPAAITELLKVPGLGPKRVKLLHDSLEVDTVEAL
                     RRAAGAGRIRELPGFGEKMEARILEALAARKAAPQRFKLAVAAQYAAALEAWLAKVPG
                     VRKVTVAGSYRRMRETVGDVDILVTAKPGSPAMERFVAYDEVKNVLAQGETRSSVVLK
                     SGLQVDLRLVPPESYGAALHYFTGSKAHNIAIRKLGQARGLKINEYGVFRGERRVAGE
                     TEESVFASVGLPWIPPELREERGEIEAARHGALPRLVTLEDLKGDLHLHTKATDGHNT
                     IREMALAAKAAGLTYIAVTEHSRRLTVARGLDPQRLAKQLDEIDRLNDELAGITVLKG
                     IEVDILENGSLDLPDSALKGLDLVVGAVHSAFTLPRRKQMERILRAMDNRYFTVLAHP
                     SARLLGERDAMDVDWLRIVRAAKERGCCLELNAQPDRLDLDDVQCRLAKEEGVLVAVS
                     SDAHSTFDFAHLKFGVGQARRGWLEPKDVLNTRGLRELKPLIAKTMGR"
BASE COUNT          488 a         1160 c         1079 g          563 t
ORIGIN      
        1 gcgtagaagc gcccgccgta gtgctcgttc tggaacgccc gctcgttgcc gagcccctgg
       61 tcgttgaaca cgacgcagac cacggggatc ttctcgcgca tcgcggtctc gagctcgccg
      121 atattgcaca tcatgcccat gtcgcccgcg gcgcacagca ccgggcggtc ggcccgcgcg
      181 agctttgcgc cgagcgcgat ggggaacgag cccccgaccg acccccagtc gtcgatgccc
      241 atgaaggtcc ccggctcgta gctcctgaag tagctgcgga cgtgcttgcc gccgttgccg
      301 gcatccacga ccagcaggcc gtcgcgcggc agcaccttgc ggatcacgtg cgcgacgaac
      361 tgcgactgga tcggcaccgc atcgctgcgc accgcggcct gcagctcggc gtcccagtcg
      421 gcgcgcgcct tcgccacgtc cacccacggc ttgcgcgggc cggatttgcc cgcgcgttcc
      481 acgagcccgg cgatgaagct cgccgtcgag cccgtcacgc cgagcgtcac cgggaacacg
      541 atgccgatct ggccgggcgc ggcgctgtga tggatgaact tcgccgactg gctgaagatc
      601 ccgtacttgt acatcgtcga gaagtagtcc aggtgcgcgc cgaacgcgat gatgaggtcg
      661 gcttgcggcg ctacgcggtt ggcgcttacc gcgccgttcc ggccgagcgg accaagcgcg
      721 agcggatggg tggacggaaa cgcgtcgggc gaatactgga gcgtcgcgac cgggacgccg
      781 gtgcgctcgg cgagctgcgc catggtgccg gtcgccgctt cgttcagcac gccgcgtccg
      841 acgacgaaaa cggggcgctc ggcgagggcg agcaactcca ttgcccgatc gagctgcgcg
      901 gggtcgcacg ccggcggcac cgtgttgcgg tacgcggccg gcgcgatcgg ctccgggacg
      961 gtcttctcca gcaggatctc gctcgaggcc tcgatatgcg ccggcccctg cggctccgcg
     1021 agcgccacgc gaaacgtctt gcgcaccgac tcctgcactt tatgcacatt gggaatgctg
     1081 tacgcccact tggtgatcgg gcgcataaag ggaacctggt ccatgtcctg gctggcgtcg
     1141 cgttcgcgca tcgcgctctc ctgcatcccg cacacgctga tcaccggcac gaaaccgcgg
     1201 tacgcggcgc cgatcgcggt gacgagattc gtgacgccgg gcccttccgt cgccgtgacc
     1261 acggccgggc gccccgaggc gcgcgcgaag ccataggcca tgaacgcggc gccctgctcg
     1321 tgacgcgtga gaatgaagcg gatccggggc gtgtcgcgca gcgcgtcgat caccgccagg
     1381 ttctgcgtgc cgggaatccc gaagacgtga tcgatgcctt cccgcaccag ggcgtcaacc
     1441 agcgcccgtg cgccggtcgt cgtgttgatc atatgttggt ccttgagatc catcgaatgc
     1501 cccttgatcg attcgcgtgg tcccgaagta tcgccccaaa atccgcccgg gacaaatgcg
     1561 cggacgccca ttcgtcaaag tcgtgatccg gaatgcgcga tgcgggatac gggacccggt
     1621 cagcggccca tggtcttcgc gatcagcggc ttcaactcgc gcagcccgcg cgtgttgagc
     1681 acgtcttttg gctcgagcca gccgcgccgt gcctgcccga cgccgaattt cagatgcgcg
     1741 aaatcgaagg tgctgtgcgc gtcggaactg acggcgacga gcaccccctc ttccttcgcc
     1801 agccggcatt gcacgtcgtc gagatcgagg cggtcgggct gggcattcag ctcgaggcag
     1861 cagccccgtt ccttcgccgc gcgcacgatg cgcagccagt ccacgtccat cgcatcgcgc
     1921 tccccgagca gccgcgcgga agggtgcgca agcaccgtga agtaacggtt gtccatcgcg
     1981 cgcaggatcc gttccatctg cttgcgccgc ggtagcgtga atgcgctgtg aacggcaccg
     2041 accacgagat cgagcccttt cagcgccgag tcgggaaggt cgaggctccc attctcgagg
     2101 atgtccacct cgatgccctt cagcacggtg atgccggcaa gctcgtcgtt caaccggtcg
     2161 atctcgtcca actgtttcgc gagccgctgc gggtcgagcc cgcgcgcgac ggtgagcctg
     2221 cgcgagtgct cggtaacggc gatgtaggtg agacccgcgg ctttggcggc aagcgccatc
     2281 tcgcggatcg tgttgtggcc gtcggtcgcc ttggtgtgca ggtgcaggtc gcctttcagg
     2341 tcttccagcg tgacgaggcg aggcagcgcg ccgtggcgtg ctgcttcgat ttctccccgc
     2401 tcctcccgca attccggcgg gatccacggc aggcccaccg aggcgaaaac ggactcctcc
     2461 gtctcgcccg ccacgcgccg ctcgccgcga aacaccccgt actcgttgat cttcagcccc
     2521 cgcgcctgcc ccagcttgcg gattgcgatg ttgtgcgcct tcgagcccgt gaagtagtgc
     2581 agcgccgccc cgtagctctc tggcgggacc agccgcaggt cgacctgcag cccgctcttc
     2641 agcacgacgc tcgagcgcgt ctctccctgc gcaagcacgt tcttcacctc gtcgtaggcg
     2701 acgaagcgct ccatcgccgg gctgccgggt ttcgccgtga ccagaatatc gacgtcgcct
     2761 accgtctcgc gcattctccg gtagctcccc gcgaccgtga ccttgcgcac gcccggcacc
     2821 tttgcgagcc aggcttcgag cgccgccgcg tattgcgcgg cgacggcgag cttgaagcgc
     2881 tgcggcgccg ccttgcgtgc ggcgagcgcc tccaggatac gcgcctccat cttctcgccg
     2941 aatccaggca gctcgcggat gcggccggcg ccggcggcgc gccgcaatgc ctcgaccgta
     3001 tcgacttcga gcgagtcgtg cagcagtttc acccgcttcg ggccgagccc ggggactttc
     3061 agcagctccg tgatcgccgc cggcagctcg cggtgcaggc gctcgagcag cgcgcaggtc
     3121 ccactgccgc agatctcctc gatcttggcg gcgaggtccg cgccgatgcc cggcagcttg
     3181 ggcagttcct tcctttcacc gatcagcgtg gcgaggtcgc ggccgagctc gttcacggtg
     3241 cgcgccgcat tgcgatacgc gcggatgcga aacgggttgt cgcccttgat
//
LOCUS       DBNGWZ010000147         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2281096, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000147 DBNGWZ010000000
VERSION     DBNGWZ010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2281096"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..660
                     /locus_tag="AC16CX_04425"
     CDS             202..660
                     /locus_tag="AC16CX_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3011793.1"
                     /translation="MKSTRISRQVKAPRAKVYSALIDADAIAKWRVPDGMTSHAHAFE
                     GREGGQFRISLTYDAPTGLGKTTAHTDTYHGRFVKLGPNERVVEVVEFETADPALRGE
                     MTITIAPADADGGTELLAVHDGLPPGLSLADNEIGWRMALAKLAALVEAD"
     gene            745..1320
                     /locus_tag="AC16CX_04430"
     CDS             745..1320
                     /locus_tag="AC16CX_04430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3011794.1"
                     /translation="MTVGKRLLMRMFGRPRGSLGRLGGIIMARMNREIAAWAIDLLGV
                     HPNDRVLEVGFGPGVGIELLAKVASSGRVVGVDYSKEMVEQAVVRNAKGVEAGRVDLR
                     EGSVERLPFEDGAFDSALAVNSMQVWPDAPAGLREIRRVMKPGGRIALAFTRHSGQPK
                     NEVVGALTAASFSEVQVADTNYGFCVLATKP"
     gene            1451..>1749
                     /locus_tag="AC16CX_04435"
     CDS             1451..>1749
                     /locus_tag="AC16CX_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3011795.1"
                     /translation="MKHTRIIVTHYGGPDALQALEEECPEPKDGEVRVRVLAAGVSLP
                     DLMMREGVHPETPPLPFTPGWDLIGVVDRLGDGISVLEPGQIVAALPISGAYAEFV"
BASE COUNT          323 a          524 c          593 g          309 t
ORIGIN      
        1 ttggtcatca acctcaagac cgccaaggct ctcggtttgg tcattccgcc ctcactgctc
       61 gtccgcgccg accaggtcat tgagtgactc cagcgagatc gtcacgactc tacactgggc
      121 tgtctaattc tagttggagg cacgatatgg ccatccggtc ggcgcgttgc tcgtctcgct
      181 aggacgcgct aacgttcgcg catgaagtcg actcgcatca gtcgtcaagt gaaagcgccg
      241 cgcgcgaaag tctacagcgc gcttatcgac gcggacgcga tcgctaagtg gagggtgccg
      301 gacggcatga ccagccacgc gcacgcgttc gagggccgcg agggtggcca gttccgaatc
      361 tcgctcacgt acgacgcgcc gaccggacta ggcaagacga ccgcgcacac cgatacgtac
      421 cacggccgtt tcgtgaagct cgggccgaac gagcgggtcg tcgaagtggt cgagttcgaa
      481 acagcagacc ccgccctgcg cggcgagatg acgatcacga tcgcgcccgc cgatgcagat
      541 ggcggcaccg agctgctcgc cgtgcacgac ggactgccgc ctggcctgtc gctcgccgac
      601 aacgagatag gctggcgaat ggcgctcgca aagctcgccg cgctcgtcga ggcggactag
      661 aagacacgcg ggcagcttcg tgccgtcaaa caaggcgttg gatggcaatc gaagtatccc
      721 cctgatgcgc gcggtcaccc ggcaatgacg gttggaaagc gtctcctgat gcgcatgttt
      781 ggtcgtcctc ggggcagcct cgggaggctg ggtggcatca tcatggcgcg catgaatcgc
      841 gagatcgccg cctgggccat cgacctgctc ggcgtccacc cgaacgaccg agtactcgag
      901 gtgggattcg gacctggtgt gggcattgag ctgctagcca aggtggcgtc gtcggggcgt
      961 gtggttggtg tcgattactc caaggagatg gtcgagcaag ccgtggttcg aaacgcgaag
     1021 ggggttgaag ctggtcgggt cgatcttcga gagggatctg tcgagcggtt gccatttgaa
     1081 gacggagcgt tcgatagtgc cctggcggtc aactccatgc aggtctggcc agatgccccg
     1141 gctgggctgc gagagatacg gcgggtcatg aagcctggcg gtaggatagc cctcgctttc
     1201 acccgacatt ctgggcagcc gaagaacgaa gtggttggtg cgctgacggc ggccagcttc
     1261 tcggaagtcc aggtggcgga cacgaactac ggcttctgtg tgctcgcgac caagccctga
     1321 aggagatgaa gatggatgcc atccaacagc gcggtagagc ggccggttgg ctcgcgttcg
     1381 ctcgccgccg ccgctcaccg cagcgttaca tcgcacaagg tcgagcgccc ggcaagcgag
     1441 gaagcggttc gtgaaacaca cgcgcatcat cgtcacccac tacggcgggc ccgatgcact
     1501 tcaggcgctc gaagaagagt gccccgagcc gaaggacggg gaagtgcggg tgagagtgct
     1561 ggccgcgggt gtttccttgc ccgacctaat gatgcgcgag ggcgttcatc ccgagacgcc
     1621 cccgctgccc ttcacgccgg ggtgggatct gatcggcgtg gtggatcggc tcggcgacgg
     1681 catctctgta ctcgaaccag gccagatagt tgccgcgctg ccgatcagcg gcgcgtacgc
     1741 ggagttcgt
//
LOCUS       DBNGWZ010000148         2907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_265557, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000148 DBNGWZ010000000
VERSION     DBNGWZ010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_265557"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..176
                     /locus_tag="AC16CX_04440"
     CDS             9..176
                     /locus_tag="AC16CX_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR3011796.1"
                     /translation="MAPKIVAKLKDGDFMRAFASKGRFSDLLATIPVHVVMNPAVGLH
                     GALLEARALVR"
     gene            complement(192..761)
                     /gene="mobA"
                     /locus_tag="AC16CX_04445"
     CDS             complement(192..761)
                     /gene="mobA"
                     /locus_tag="AC16CX_04445"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HIR3011797.1"
                     /translation="MILAGGQGRRMGGVDKGLKLLRGKPMIAWVIERFAPQVAEVLIN
                     ANQNREAYARFGYRVIPDEISGFAGPLAGLHRGLTEATHELVATVPCDSPFLPHDLID
                     RLRAALERGGAEVAVARTGDHPHPVFCLCRKRVLPGLTGFLAGGGRKIDAWYAKLKVV
                     EVPFDDQPDAFSNVNTEAELKAFEEGPLP"
     gene            complement(866..1693)
                     /locus_tag="AC16CX_04450"
     CDS             complement(866..1693)
                     /locus_tag="AC16CX_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR3011798.1"
                     /translation="MYRPTLTNAGRPLTFEVEAVNERGERLPTPIAGEHPLTLYLDKR
                     EIVTLMTLGQAPEALTIGYLRNQRLVGSIEEIAAVQVDWETDSVAVTTRKGVKGLRER
                     LKKRTVTTGCGQGTVFGDLMEEIESIKLRDDVQLEEDTLYALLDAVRRHETIYKSAGA
                     VHGCALAAGAEILYFVEDVGRHNAVDAIAGQMWLDRVDGSDKIFYTTGRLTSEMVIKA
                     AQMQIPFLVSRSGLTEMGHEIANKVGITMLGRAVGKHYLLFTGNHRFVKQIMPTMLA"
     gene            complement(1789..2808)
                     /gene="moaA"
                     /locus_tag="AC16CX_04455"
     CDS             complement(1789..2808)
                     /gene="moaA"
                     /locus_tag="AC16CX_04455"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3011799.1"
                     /translation="MTTGSPVPQDRLGRALRDLRISVTDRCNFRCVYCMPKEVFGKDY
                     QFLERKEILTFEEIARLAGVFGELGVEKIRLTGGEPLVRRNLEQLVGMLARMPGLDLT
                     LTTNGSLLKQKARALKEAGLRRITVSLDSLDDATFRRMNDVDFPVGRVLEGIEAAAAA
                     GLEPVKINMVVKRGENEHGILPMARFFRGTGRILRFIEYMDVGHTNGWRMDDVVTARE
                     IIEMINREMPLEPLDPNYPGEVAERWCYRDGSGEIGVIASVTQAFCRDCTRIRLSTEG
                     KLYTCLFATDGHDLRVLLRGGASDSEIRSTIEAIWSKRSDRYSEIRTAHTAKLQKIEM
                     SYIGG"
BASE COUNT          491 a          932 c          930 g          554 t
ORIGIN      
        1 cgggtgggat cgcgcccaag atcgtggcga agctcaagga cggcgacttc atgcgcgcat
       61 tcgccagcaa aggccggttc agcgacctgc tggctacgat acccgtgcac gtcgtgatga
      121 atccagcagt cggcctccac ggcgcgctgc tcgaagcgcg cgcgctggtc cgataggcct
      181 gtgacccatc ctcatggtag cggcccttcc tcgaaggctt ttagttcagc ttccgtgttg
      241 acgttgctga aggcgtcggg ctggtcgtcg aatggcacct cgaccacctt caattttgcg
      301 taccaggcat cgatcttgcg gcctcccccc gcaagaaatc cggtcaggcc gggcagcacc
      361 cgcttgcggc acaggcagaa cacggggtga gggtggtcgc cggtcctggc caccgcgact
      421 tcagcgccgc cgcgttcgag cgcggcgcgc agccggtcga tcaggtcgtg cggcaggaaa
      481 ggcgaatcgc acggcacggt tgccaccagc tcatgtgtcg cttcggtgag cccccggtgc
      541 agaccggcga gcgggccggc gaaaccggaa atctcgtccg ggatcacgcg gtaaccgaag
      601 cgcgcgtacg cctcgcgatt ctggttggcg ttgatcagga cctcggcgac ctggggcgcg
      661 aaacgctcga tcacccacgc gatcatcggc ttgccccgca ggagcttcaa acctttgtcc
      721 accccgccca tgcggcggcc ctggccaccg gcgaggatca cgccagtgac gagggatgaa
      781 ggatgaggga tgagggatga atttcgcgta gaagggttca agatcgaccc cagccaaaat
      841 cagcccggta ttccgctaaa gcaaatcatg ccagcattgt tggcatgatt tgcttcacga
      901 agcggtggtt gccggtgaac agcaggtaat gcttgcccac cgcgcgcccg agcatggtga
      961 tgccgacctt gttcgcgatt tcgtggccca tctcggtcag gccggaacgc gacaccagga
     1021 aggggatctg catttgcgcc gccttgatca ccatctcgga agtgagccgc cccgtggtgt
     1081 agaaaatctt gtccgagccg tccacgcggt cgagccacat ctggccggcg attgcgtcca
     1141 ccgcgttgtg gcgcccgacg tcctcgacga agtagaggat ttccgcgccg gcggcgagcg
     1201 cgcagccgtg caccgctccc gcgctcttgt agatcgtctc gtggcgccgc accgcatcga
     1261 gcagcgcgta gagcgtgtct tcctcgagct ggacgtcgtc gcgaagcttg atggattcga
     1321 tctcctccat caggtcgccg aacaccgtgc cctgcccgca gccggtggtg acggtgcgct
     1381 tcttcagcct ttccctgagc cctttcacgc ccttgcgcgt ggtgacggca accgaatccg
     1441 tctcccaatc cacctgcacg gcggcgattt cctcgatcga gccgaccagg cgctggttgc
     1501 ggagataacc gatggtgagc gcctccggcg cctggccgag cgtcatcagg gtgacgatct
     1561 cgcgcttgtc gagataaagc gtgagcggat gctccccggc gatcggcgtg ggcaggcgct
     1621 cgccgcgctc gttcacggcc tcgacctcga aggtcagcgg ccggccggcg ttggtgagcg
     1681 tgggacgata catatcaagg cggaaggcag aaggataacc acgaattgtg gcggattccg
     1741 gttttccttc atccttctgc cttcttcctt catccttcgg aggcggcgtc agccgccgat
     1801 gtaggacatc tcgatcttct gcagcttggc ggtgtgggcg gtgcggatct cggagtagcg
     1861 gtcggaccgc ttcgaccaga ttgcctctat cgtgctccgg atctcgctgt cggaggcgcc
     1921 gccgcgcagc agtacccgca ggtcgtgccc atcggttgcg aacaggcagg tgtagagctt
     1981 gccctcggtg gagagccgga tgcgggtgca gtcgcggcaa aacgcctggg tgaccgacgc
     2041 gatcacgccg atttcgccgc tgccgtcccg gtaacaccag cgctccgcga cttcgcccgg
     2101 atagttggga tcgaggggct cgagcggcat ttcacggttg atcatctcga tgatctcgcg
     2161 cgcggtgacg acgtcatcca tgcgccatcc gttggtgtgc ccgacgtcca tgtactcgat
     2221 gaaacgcagg atgcgcccgg tgccgcggaa aaaacgcgcc atcggcagga tgccgtgctc
     2281 gttctcgccg cgcttgacca ccatgttgat cttgaccggt tcgagtcccg cggccgccgc
     2341 cgcctcgatt ccttccagca ccctgccgac ggggaagtcc acgtcgttca tgcgccggaa
     2401 cgtcgcgtcg tccagcgagt cgaggctgac ggtgatgcgc cgcaggcccg cttccttgag
     2461 cgcacgcgct ttctgcttga gcagcgagcc gttggtggtg agcgtgagat cgagcccggg
     2521 catccgtgcc agcatcccga cgagctgctc gagattgcgc cggaccagcg gttcgccgcc
     2581 ggtcagacgg attttctcga cgccgagttc gccgaacacg ccggcaagcc gggcgatctc
     2641 ctcgaacgtg aggatctcct tgcgctcgag gaattgatag tccttgccga atacctcctt
     2701 cggcatgcag taaacgcagc ggaaattgca gcgatcggtc accgagatgc gcagatcgcg
     2761 cagcgcgcgc cccaaccgat cctgtggcac tggtgaaccc gtggtcatcg gtcgtcctgg
     2821 ttttcgtcca tgggggtcat cttaaatcac acggcgcagt gcttctcatg cgccgtgcga
     2881 ttggcgatta tgacaaagga gccggcc
//
LOCUS       DBNGWZ010000149         2107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_391584, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000149 DBNGWZ010000000
VERSION     DBNGWZ010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_391584"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16CX_04460"
     CDS             complement(<1..365)
                     /locus_tag="AC16CX_04460"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR3011800.1"
                     /translation="MNAEALIGLVMLLVMIGAIFIGFPISFTLLFLALAFGYFSLGGM
                     VFDLAYLQTLGMMKEELFAAVPLFIFMGFITEQAGLMERLFTAFRMLLAPIRGSLFAV
                     VILTATVFAMATGIVGAAVT"
     gene            complement(362..907)
                     /locus_tag="AC16CX_04465"
     CDS             complement(362..907)
                     /locus_tag="AC16CX_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR3011801.1"
                     /translation="MPPLHIRIVRAIDRFTDSTGTLVAWLNVPLMLIVAYEVIARYVF
                     EAPTIWSFDLTYMLYGTIFMLGAAYALHMGAHIRTDFFFEKWSVRTRGWIDTVAYIGF
                     FFPALIVLLAVSWGEGWYAFQIGEVSEQTPWRPILWPFKMVVPLTCLLLMVQGVSESV
                     KSVYAALTGIELEHKEKVVEL"
     gene            complement(1089..>2107)
                     /locus_tag="AC16CX_04470"
     CDS             complement(1089..>2107)
                     /locus_tag="AC16CX_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3011802.1"
                     /translation="LTLQDNFRMFADRVDKLTGGQLKIDALAAGQVVPAFEVLDATSK
                     KVIDGAHSVSYYWVGKNKAATLFASTPAGPFGMDTMDFMGWLYEGGGLDLWWEFYQKE
                     LKLNVIAFPILPAGPQAFGWFKRPIKNLADFKGMKCRQTGIVAEIFQRMGMQTVNMPG
                     GEIVPSAQRGVIDCAEWVGGIEDLRLGLPQVWKYHYTPGMHEPSIVGELIINLDVWKS
                     LTPQQQEAIRSATSETFVRWWIKWQKQNADAIEEMRTKYGTQILRTPPDILIAFLKAW
                     DEIAKEESAKNPFFKKVLDSQRAYASKVVPAKRFMFPPYSFAANYYWPEKAAPKAAVK
                     GEKK"
BASE COUNT          416 a          678 c          633 g          380 t
ORIGIN      
        1 gtgaccgccg cgccgacgat gccggtcgcc atcgcgaaga cggttgcggt caggatgacc
       61 accgcgaaca gcgagccgcg gatcggcgcg agcagcatgc ggaacgccgt gaacaaccgc
      121 tccatcagcc ccgcctgctc ggtgatgaac cccatgaata tgaacagcgg caccgccgcg
      181 aacagctcct ccttcatcat gcctagcgtc tgcaggtaag cgaggtcgaa caccatcccg
      241 cccaggctga agtagccgaa ggcgagcgcg aggaagagca gcgtgaagct gatcgggaag
      301 ccgatgaaga tcgccccgat catgaccagc agcatgacca gtccgatcag ggcttcggcg
      361 ttcatagttc gacgaccttc tccttgtgtt ccagctcaat gccggtcagc gcggcgtaca
      421 cgctcttcac gctctccgag acgccctgga ccatcagcag caggcaggtg agcggcacga
      481 ccatcttgaa cggccacagg atcggccgcc acggcgtctg ctcggagact tccccgatct
      541 ggaacgcgta ccagccctcg ccccagctca ccgcgagcag cacgatgagc gctggaaaga
      601 agaatccgat gtacgccacc gtgtcgatcc agccgcgggt tctgaccgac cacttctcga
      661 aaaaaaagtc ggtgcggatg tgcgcgccca tgtgcagcgc gtaggccgcg ccgagcatga
      721 agatcgtgcc gtagagcatg taggtcaggt cgaacgacca gatcgtgggt gcttcgaaca
      781 cgtagcgcgc gatcacctcg taggcgacga tcagcatcag cggcacgttg agccacgcga
      841 cgagcgtccc ggtcgagtcg gtaaaccggt cgatcgcgcg cacgatcctg atatggagcg
      901 gcggcatatt tgaaaaagcg ggacttggga ctagggacta ggggctggaa aattcaggcc
      961 cgggatcgat gataaacctg aaatttcgac tgtcgagtcc cgagccccga gccccgcatc
     1021 ggcttcaccg tcatccctaa aaaagaaaaa ggcggtcacg ccgccttttt cccgatctgg
     1081 agcatgcgtt acttcttctc gcccttcacc gccgctttcg gcgccgcctt ctcgggccag
     1141 tagtaattgg ccgcgaacga atacggcgga aacatgaagc gcttggcggg caccaccttc
     1201 gaggcgtagg cgcgctgcga atcgagcacc ttcttgaaga acgggttctt cgccgattcc
     1261 tccttcgcga tctcgtccca ggccttgagg aacgcgatca ggatgtcggg cggcgtgcgc
     1321 aggatctggg tcccgtactt ggtgcgcatc tcctcgatcg catcggcgtt ctgcttctgc
     1381 cacttgatcc accaccgcac gaacgtttcc gacgtcgccg agcgtatcgc ttcctgctgc
     1441 tgcggcgtga ggctcttcca gacgtcgagg ttgatgatca gctcgccgac gatgctcggc
     1501 tcatgcatgc cgggcgtgta gtggtacttc catacctgcg gcaggccaag ccgcaagtcc
     1561 tcgatgccgc ccacccattc ggcgcagtcg atcacgccgc gctgggcgga gggcacgatc
     1621 tcgccacccg gcatgttgac cgtctgcatg cccatgcgct ggaaaatctc ggcaacgatc
     1681 cccgtctggc ggcatttcat ccccttgaag tcggcgaggt tcttgatcgg tcgcttgaac
     1741 cagccgaacg cctgcgggcc ggcgggcagg atcgggaagg cgatgacgtt gagcttgagc
     1801 tctttctggt agaactccca ccacaggtcc agcccacccc cttcgtataa ccagcccata
     1861 aagtccatcg tgtccatgcc gaagggaccg gcgggagtgg aagcgaacag ggtcgccgcc
     1921 ttgttcttgc ccacccagta gtaggacacc gagtgcgcgc cgtcgatgac tttcttggag
     1981 gtcgcatcca gcacctcaaa cgcaggcacc acctggccgg cggcgagcgc gtcgatcttg
     2041 agctgcccgc cggtgagctt gtccacgcgg tcggcgaaca ttcggaaatt gtcctgcagc
     2101 gtcaggc
//
LOCUS       DBNGWZ010000150         3460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1021675, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000150 DBNGWZ010000000
VERSION     DBNGWZ010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1021675"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="AC16CX_04475"
     CDS             <1..489
                     /locus_tag="AC16CX_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3011803.1"
                     /translation="MGFTTRDTLFLILVVNVTAAAGAFLFGQVQDRFGHVRTLAVTLI
                     GWVLAVLLAWAAEGPLLFWVSANVVGVCLGSSQSAGRALVGYLSPPARTAEFFGLWGL
                     AVKLSSILGPLTYGLVTWITQGNHRLAILVTGLFFIIGLAILARLNVQRGRAVALEEK
                     AR"
     gene            586..2133
                     /locus_tag="AC16CX_04480"
     CDS             586..2133
                     /locus_tag="AC16CX_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011804.1"
                     /translation="MAINWLGGGKADHPMADPKRAREILADLPANNSPKALEEIVEWL
                     ESVTQVEDFKLDRRLEAIDLLDGAAKTHQRKITQEYLATQRHQKFQENRIWNCSYDFW
                     RRLGEAYVLCIKHHESGFSGASTVKKILPMIVARALRALTLQVKWTLLRYGPVEPRVF
                     QEMSRLYQFAETKGFVDDMVAIYPGAHGQGSVKQEFLKAIMLSASSTDGLTPARQEIA
                     ERAVAHFSGAFQLSKTADGLNFCFNLAEPRAPVRRITVKDAVPGLRYFGAGEGLAQLN
                     ALIARIQQTGAIPADINLGGIQEKNLVLSVLKHLAQYWSDKPPARSSERRPTAGSISV
                     VSGMTEILGMLDPSSSGDLDFSRDSSAQTTENWVVVNVSEGGYGVIVPPKKSDWLKVG
                     VLVGIQSETSKHWGVGLVRRITSDEHQQRRVGIQLLTKAALPIKVGKASSSTAASDLQ
                     PAILLSTSPDAKGEVGVILREGLFNGRDSLDMMVRDKTYLLMPSSMVEDGEDFDWARF
                     KVMQRSA"
     gene            complement(2219..2896)
                     /locus_tag="AC16CX_04485"
     CDS             complement(2219..2896)
                     /locus_tag="AC16CX_04485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3011805.1"
                     /translation="MEKLLETIEIETGKNPGVSVIWMHGLGADGNDFVPVVSELGLDA
                     APAIRFVFPHAPMRPVTINNGYVMRAWYDVSFGDLEGNSRHADERGVRESQAQINALI
                     EREGKRGIAAGNVVLAGFSQGGAIALQTGLRHPERLAGVMALSTYLPLADSFPREASA
                     ANKDTPIFMAHGLFDPLVPLVMGAGSMTFLTGLGYAVEWHQYPMQHSVCAEEIRDIGA
                     WLRRVLG"
     gene            complement(3181..>3460)
                     /locus_tag="AC16CX_04490"
     CDS             complement(3181..>3460)
                     /locus_tag="AC16CX_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011806.1"
                     /translation="SAHHYFVTGFEDRKDDEYQRLTHLETKIQLMLNAKEDDHIQLEK
                     LIREMIFSLERGKDGDATFVNVHPKIIALSRQILKREWNVVKEPIAVA"
BASE COUNT          669 a         1045 c         1091 g          655 t
ORIGIN      
        1 atgggcttta ccacgcgcga cacgctgttt ctgattctgg tggtcaacgt gacggcggcg
       61 gcgggcgcgt tcctgttcgg gcaggtgcag gaccggttcg gacatgtgcg cacgctcgcg
      121 gtgacactga tcggctgggt tctggcggtt ttgctggcgt gggcggcgga agggccgttg
      181 cttttctggg tctccgccaa tgtggtcggg gtatgcctcg gctccagcca gtccgcaggc
      241 cgcgccctgg tcggctacct cagccccccc gcgcgcaccg ccgagttctt cgggctgtgg
      301 gggcttgcgg tgaagctttc ctcgattctc ggtccgctca cctacgggct ggtgacctgg
      361 attacacagg gtaatcaccg gcttgccatc ctcgtcacgg gcctgttttt catcatcggc
      421 ctcgcgatcc tggccaggct gaacgtccag cgcggcagag ccgtcgcgct ggaggaaaaa
      481 gcacgatagg tgacaggtat cgcgcctttt ccgggcgtag cggttactct acaattcctc
      541 cctcacttca agctcgtcgt gttacggggc agttcagggg gcaaaatggc gatcaactgg
      601 ctgggcggcg gcaaggccga tcaccccatg gccgacccga agcgggcgcg cgagatcctg
      661 gcggatcttc cggcgaacaa ctcaccgaaa gcgctcgagg agatcgtcga atggctcgag
      721 tccgtgactc aggtcgagga cttcaagctc gaccggcgcc tggaagccat cgacctgctg
      781 gatggcgccg ccaagaccca ccagcgcaag atcacgcagg agtacctggc gacgcagcgg
      841 caccagaaat tccaggaaaa caggatttgg aactgctcgt atgatttctg gaggcgcctg
      901 ggcgaggctt acgtcctatg catcaagcac catgaaagcg gattcagcgg cgcctcgacc
      961 gtcaagaaga tcctgccgat gatcgtcgcg cgggcgctgc gcgcgttgac cctgcaggtc
     1021 aaatggacgc tgctgcgtta cgggccggtc gagccgcggg tatttcagga gatgtcgcgc
     1081 ctctaccagt tcgcggagac gaagggcttc gtcgacgata tggttgcaat ctacccgggc
     1141 gcgcatggac agggctccgt gaagcaggaa ttcctaaaag cgatcatgct ctccgcctcc
     1201 tccaccgacg ggctgacgcc ggcgaggcag gagatcgcag aacgcgcggt cgcgcatttt
     1261 tccggcgcgt tccagttgtc gaagaccgcg gacgggctta atttctgctt caacctcgcg
     1321 gaacccaggg caccggtgcg acggatcacg gtgaaagatg ccgttccggg cctgcgttac
     1381 ttcggggccg gggaagggct ggcgcagctg aatgcgctga tcgcgcggat tcagcagacg
     1441 ggcgccatac ccgccgacat caatctcggc ggaatccagg aaaaaaatct ggtactcagc
     1501 gtgctcaagc atctggcgca gtactggtcg gataagccgc cggcccgcag ctcggagcgg
     1561 cgcccaactg ccggcagcat ttccgtcgtg tccggaatga ccgagatcct cggcatgctc
     1621 gatccgtcat cgagcggtga tctcgacttc agccgggact cgtccgcgca gacgaccgag
     1681 aactgggtcg tggtcaacgt gagcgagggg ggctatggcg tgatcgtccc gcccaagaaa
     1741 agcgactggc tcaaggttgg cgttctggtt ggcatacaga gcgagacttc caagcactgg
     1801 ggtgtcgggc tggtgcggcg catcacatcc gacgaacatc agcagcggcg ggtcgggatc
     1861 cagttgctca cgaaggcggc gctcccgatc aaggtgggga aggccagctc cagtaccgcc
     1921 gcgagcgacc tgcaaccggc gatcctgctc tcgacctccc ccgacgccaa gggtgaggtc
     1981 ggggtgatcc tgcgcgaggg cctgttcaac ggccgggaca gtctcgacat gatggtgcgc
     2041 gacaagacct atctcctgat gccgagcagc atggtcgagg acggcgagga cttcgactgg
     2101 gccaggttca aggttatgca gcgcagcgcc tagcgcgctg cgcggtgagg cgtgaggcgt
     2161 gaggggtgag gtttgagttc cgccttacgc ctgacgccta actcctcacc gctcggcgtc
     2221 agccgagcac ccggcgcagc caggcgccga tgtcccggat ctcctccgca cagaccgagt
     2281 gctgcatcgg gtactggtgc cattcgacgg catagccgag gcctgttaga aacgtcatcg
     2341 atccggcgcc catcaccagc ggcacgagcg gatcgaacag accgtgcgcc atgaagatcg
     2401 gcgtgtcctt gttggccgct gaggcttccc gcggaaacga atccgcgagc ggcagatagg
     2461 tcgagagcgc catcacgccg gcgagccgtt ccgggtgtct caagccggtc tgcagcgcga
     2521 tcgcgccgcc ctgcgagaag cccgcgagca cgacattgcc tgccgcgatg ccgcgcttgc
     2581 cctcgcgctc gatcagcgcg ttgatttgcg cctgcgactc gcgcacgccc cgctcgtcgg
     2641 catggcgcga atttccttcg aggtcgccga aagagacgtc gtaccaggcc cgcatcacgt
     2701 agccgttgtt gatcgtcacc gggcgcatcg gcgcgtgcgg gaacacgaag cggatggcgg
     2761 gcgccgcgtc gagcccgagc tcgctcacga ccgggacgaa atcgttgccg tcggcgccga
     2821 gcccgtgcat ccagatcacg gaaacaccgg ggttcttgcc ggtttctatc tcgatcgttt
     2881 ccagtaattt ttccatccaa ccgccaggga gaacaaaata acgtaagggc gggagagcat
     2941 cagagcggga gatgggaagg taagccccct ctcacgattt cccgatcttt cgatctcacg
     3001 gcttcttgaa ccgccaagac gccaagagaa tcaagaacga taatgaaccg ccaagatcgc
     3061 caagacaatc agaaacaaaa ttgaaccacc gagcacgcag agagattcaa tgatcgagca
     3121 gattaacgtg aaggtaacca gcctgcgcgg cttttcgcgc aggtccggtt gaccgcaagg
     3181 ttatgccacc gcgataggct ctttgacaac attccattcc ctcttaagaa tctgacgaga
     3241 cagcgcaatg attttcgggt ggacattaac aaacgtcgca tcgccgtctt ttcctctttc
     3301 aagcgaaaaa atcatttcgc gaatgagctt ttcgagctgg atgtgatcgt cttctttggc
     3361 attcagcatg agttgaatct tcgtctccaa gtgcgtgagc cgttggtatt cgtcgtcctt
     3421 tctgtcctca aagcccgtta cgaagtagtg atgtgcactg
//
LOCUS       DBNGWZ010000151         4210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3036817, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000151 DBNGWZ010000000
VERSION     DBNGWZ010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3036817"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="AC16CX_04495"
     CDS             <1..359
                     /locus_tag="AC16CX_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011807.1"
                     /translation="DIRCRKGCDHCCHGPVEIWPHEAALLVEIARGAGMEMDKARLER
                     QSRYTMDNWRQQPAADRACVFLGDDGECVRARGRAGSGGVAGVGRVLRATRCRWKPWP
                     IPCSCPLFGNPVYDCA"
     gene            371..1723
                     /locus_tag="AC16CX_04500"
     CDS             371..1723
                     /locus_tag="AC16CX_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011808.1"
                     /translation="MGRYQRGFVLKALSWMAGLVLLAAAAVFGINATDEELSELAQKA
                     MAVPPVPPASAGNGYTDFLALDGDDIDQRLKRACGQEPDGCLKLAADNPELVQLNARP
                     DAFTAAYRAMRAKPEFVDLHESKGPDDFIPSYQHLIWGQGRSLLLAAGLANQGKLEAA
                     VAELEAGNAFHRRVAAGSRSLITRMIAFILLQRDAFFASNLARIPGRRATAVLGRLKA
                     IVRPLSKAELDVRRSLEVERAQHISWMRTRSYVRLSDDYYRISKELEGQEISRPWWDP
                     VAPYLYRPHQSVNRYAAQTEILLGVAALPATEFAAGAAAAAEKARALAPQGLASLFIN
                     PVGREHPYVSVMPGLMGNADAYTPYIGRAHATQSVYTLVTLQIRLREAGITSLSAVER
                     ALDGPLFMSHHDPFTGKPMRFDAKTRTIGFDSPVRFGGSLEFIRKRYGRVAVELALDR
                     "
     gene            1827..1970
                     /locus_tag="AC16CX_04505"
     CDS             1827..1970
                     /locus_tag="AC16CX_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011809.1"
                     /translation="MYWIQKMEELRKQMEQEELERQRKPATAPAAPAKPTAGAEEPQP
                     LPV"
     gene            complement(2101..2511)
                     /locus_tag="AC16CX_04510"
     CDS             complement(2101..2511)
                     /locus_tag="AC16CX_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011810.1"
                     /translation="MNLTFQVRHSNPAGLWTSPRLTQMVEVQGARMIYLSGQTAADAN
                     YRVGSSDFRWQAHAVYDNIELALRSAGATLQNIVKTTTYLTNAEHLAALREVRLERYK
                     DLQAPPANTLLVVSRLAEPEFLIEIDVVAALPAR"
     gene            2594..3556
                     /locus_tag="AC16CX_04515"
     CDS             2594..3556
                     /locus_tag="AC16CX_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011811.1"
                     /translation="MNVLRVACVVCAIVWSAPAAAQGYPEKPVRMIVPFPAGGGSDTA
                     GRAISERLSERLGQPVVVENRVGAAGSIGAEFAARAPADGYTILLGSTSELTQYPLVN
                     RKIRYDPVRDFVPISMVGTIPLVLAVHASLPANSVADLIRLARARPGQIDFGSAGVGA
                     TTHLAVEHFMLLTKVKLRHVPYKGSPQVSADLVAGHIQMAIPTLPAAFPFIQSRRVKA
                     LAVTTEKRVPVLPEVPTMQEAGVKGYVNVLWAGVLAPRGTPGAIVKRLHGEIVSALET
                     ARVRKVLEANGATPQSSTPEEFAAFIKSELDKWERVVKEAGVTL"
     gene            complement(3564..>4210)
                     /locus_tag="AC16CX_04520"
     CDS             complement(3564..>4210)
                     /locus_tag="AC16CX_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011812.1"
                     /translation="RRKLAPALEGAGASLASVVKAQVYLADIHDVPAFNQVWARHFGE
                     AMPATTFVPTLDPGFAIADARCEINLVAVTRDGVTPRTPLQGSCPAVCDGHPLAVRAG
                     SLLFFSGLVAADAKGPIPGTRRGAGRSRSGIRAQMNYLLDIAAEACRRSGTSLENVLR
                     IQQFHTDLGDFEPACRIWLERLGGRPLPISAVQVPGPLVVPGCTVMLDLWVYVP"
BASE COUNT          707 a         1351 c         1453 g          699 t
ORIGIN      
        1 gcgacatccg gtgccgcaag gggtgcgacc attgctgcca cgggccggtc gagatctggc
       61 cgcacgaggc tgcgctgctg gtcgagatcg cgcgcggggc gggcatggag atggacaagg
      121 cgcgcctcga acgccagagc cggtacacca tggacaactg gcggcagcag ccggcggccg
      181 acagggcctg cgtgttcctg ggcgatgacg gcgaatgcgt gcgagctcga ggccgcgccg
      241 ggagcggagg tgttgcaggt gtaggacggg tgctgcgcgc tacgcgatgc cgctggaaac
      301 cgtggcctat cccctgttca tgtcccctat ttggcaatcc agtttatgat tgcgcgtagg
      361 ggagggggac atgggtcggt atcagcgggg attcgtattg aaggccttgt cctggatggc
      421 tgggctggtg cttctcgcgg ctgccgccgt cttcggcatc aatgccacgg acgaggaatt
      481 gagcgaactc gcccaaaagg ccatggcggt gccgccggtt ccgccggcgt cggcgggcaa
      541 cggctacacg gatttcctcg ccctggacgg cgacgacatt gaccagcggc tcaagcgggc
      601 ctgcggtcag gaaccggacg gctgcctgaa acttgccgcc gacaatccgg aactggtgca
      661 gctgaatgcc cgccctgatg cgttcacggc tgcttatcgg gcaatgcgtg cgaagccgga
      721 gttcgtggat ctccacgaat cgaagggccc ggatgatttt ataccttcgt atcagcatct
      781 gatctggggg caggggcggt cgctgctcct ggctgcgggc ctggcgaacc agggcaagct
      841 cgaggccgcc gtagcggagc tcgaggccgg gaacgcgttt caccgccggg tcgccgccgg
      901 gtcgcggtcc ctgatcacca ggatgatcgc gttcatactt ctgcagcgcg atgctttttt
      961 tgcatccaac ctcgcgcgta tcccgggcag gcgggcaacg gccgttctcg ggcggctgaa
     1021 ggcgatcgtg cgtccattgt cgaaagcgga actcgacgtg cgtcgaagcc tcgaagtcga
     1081 gcgcgcgcag cacatctcct ggatgcggac ccggagctat gttcgcttgt cggacgatta
     1141 ctacagaatt tcaaaggagc ttgaggggca ggaaatatcg cgcccatggt gggacccggt
     1201 cgcgccctac ctctatcgcc cgcaccagag cgtcaatcgc tatgcggcac agaccgagat
     1261 cctgctcggc gtggcggcgt tgccggccac ggaattcgcc gccggggcgg ccgcggcggc
     1321 ggagaaagcg cgcgcgctcg cgccacaagg gctcgccagc ctgttcatca accccgtggg
     1381 tcgcgagcac ccgtatgtct ccgtcatgcc cggattgatg ggaaatgccg acgcgtatac
     1441 gccctacatc gggcgcgcgc acgcgacgca gtcggtctac acgctggtta cgctgcagat
     1501 ccggctgcgc gaggcaggga tcacgagcct gtccgcggtc gaacgcgcgc tcgacggccc
     1561 attgttcatg tcgcaccacg atccgttcac gggcaagccg atgcgcttcg acgccaaaac
     1621 gcggacgatc ggattcgatt caccggtgcg tttcggaggc tcgctggagt tcatccgcaa
     1681 gcgctacggc cgcgtggcgg tggagttggc gctcgatcgt tgagttcgat ccgttacggc
     1741 ggttccttag cgtcgattta gcggggtgga cagtttgtgc ttgttgagat gaaggcgtag
     1801 atccgggata aattgagcct gaccggatgt actggatcca gaagatggag gagttgcgga
     1861 agcagatgga gcaggaagag ctggagcgcc agcgcaagcc ggccaccgcg cccgccgcgc
     1921 cggcgaagcc cacggccgga gccgaagagc cgcagccgct gcccgtgtag tcgctgttac
     1981 cctcaatgac aaagccccgc cggagcgggg ctttttgttt cagcaaaacc gtggtctgcc
     2041 ccgatttgcc cgatttgttt acacgcggcg gataatgggc gaccccgaaa tagccggcaa
     2101 ctaccgggcc ggcagcgcgg ctacaacgtc gatctctatc aggaattccg gctccgccag
     2161 acggctcacc acgagcaggg tgttggccgg aggcgcctgc aggtccttgt agcgctccag
     2221 ccgcacctcg cgcaaagccg cgaggtgctc cgcgttcgtc aggtaggtcg tggtcttgac
     2281 gatgttctgc agcgtcgcgc ccgcggagcg cagcgcaagc tcgatgttgt cgtacaccgc
     2341 atgggcctgc cagcggaagt cgctgctgcc cacccggtag ttcgcgtcgg ccgccgtctg
     2401 gcccgataga tagatcatcc gcgcgccctg gacctcgacc atctgcgtga gccgcggcga
     2461 agtccacaat cccgccgggt tgctgtgtct cacctggaac gtcagattca tggagtcccc
     2521 cgtgtagcgg cctgctggtt cgacgaatgg ggcggccatg ctagcataag gtcgagttcg
     2581 ggagggacga gccatgaacg ttctgcgagt cgcctgcgtc gtatgcgcca tcgtgtggag
     2641 tgcgcccgcc gcggcgcagg gttatcccga gaagccggtg cgcatgatcg tgccgtttcc
     2701 ggccggcggc ggctcggata cggcggggcg cgccatctcg gagcgcctga gcgagcggct
     2761 ggggcagccg gtcgtcgtcg agaaccgggt cggcgccgcc ggcagcatcg gcgcggaatt
     2821 cgcggcccgg gcgccggccg acggctacac gatcctgctc ggctcgactt cggagctgac
     2881 gcagtacccg ctggtcaacc gcaagatccg ctacgacccg gtgcgcgatt tcgtcccgat
     2941 cagcatggtg ggcaccatcc cgctcgtgct cgccgtgcac gcgtcgctgc cggcgaactc
     3001 cgtggccgat ctcatccgcc tggcgcgtgc ccgccccggg caaatcgatt tcggctcggc
     3061 gggcgtcggc gcgacgacgc acctcgccgt cgagcacttc atgctgctca ccaaggtgaa
     3121 gttgaggcac gtgccctaca agggaagccc gcaggtgtcc gcggacctgg tggcgggcca
     3181 catccagatg gccatcccca cgctgcccgc ggcatttccg ttcatccagt cgcggcgcgt
     3241 caaggcactg gccgtgacga ccgagaagcg cgtcccggtg ttgccggaag tcccgacgat
     3301 gcaggaagcg ggcgtgaagg gctatgtcaa cgtgctgtgg gcgggcgtgc ttgcgccgag
     3361 aggcacgccg ggcgccatcg tcaagcgcct gcacggcgag atcgtgagcg ccctcgagac
     3421 cgcacgggtg cgcaaggtgc tcgaagccaa tggcgcgacc ccgcagtcga gcacaccgga
     3481 agaattcgca gcgttcatca agtccgagct ggacaagtgg gagcgggtcg tcaaggaggc
     3541 gggcgttacg ctgtgactcc ccgtcacggc acgtagaccc acaggtcgag catcacggtg
     3601 cagcccggca ccacgagcgg gccgggaacc tgaaccgccg agatgggcag cggccggccg
     3661 cccagccgct cgagccagat ccggcacgcc ggctcgaaat ccccgagatc cgtatggaac
     3721 tgctggatcc gcagcacgtt ctccagagac gtgcccgaac gccggcatgc ctccgccgcg
     3781 atatcgagga ggtaattcat ctgcgcccgg atgccggaac gcgagcgccc ggcgccgcgg
     3841 cgcgttcccg ggatcggccc cttcgcatcg gcggccacga ggccggaaaa gaaaagcagg
     3901 cttcccgccc gcaccgcgag tggatggccg tcacacaccg ccgggcagct tccttgcaac
     3961 ggcgttctcg gggtgacgcc gtcgcgggtc accgccacca ggttgatctc gcagcgcgcg
     4021 tccgcgatcg cgaagccggg atcgagggtc ggcacgaagg tggtcgcagg catcgcttct
     4081 ccgaaatgcc gcgcccagac ctggttgaac gcgggaacgt cgtggatgtc cgccagatag
     4141 acctgcgcct tcacgacgct cgcgagcgag gcgccggcgc cttcgagcgc gggcgcgagc
     4201 ttcctgcgga
//
LOCUS       DBNGWZ010000152         3956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1652703, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000152 DBNGWZ010000000
VERSION     DBNGWZ010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1652703"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1037
                     /locus_tag="AC16CX_04525"
     CDS             78..1037
                     /locus_tag="AC16CX_04525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011813.1"
                     /translation="MITRLTAALALLLAAAYGYAQDYPTRAITIVVPNPPGGMNQIHA
                     QPLSAVVEKLTSQPAPVVNRPGGTAAVGTASVVNAPPDGYSILVTTPNLYLVIEKDKL
                     YGIKSPFSLDQIALLALLSADPLIMVVHPSLPVKTAKQLVALAKSKPNELVFSTSGPY
                     GITHTPQALFMEATKTKMRHLPTTGGGPAITQALGGHAQVTGGGPAAIYAYVKSGKLR
                     PIASWGTKPHPSMPEVPTFKSQGIDIEAYLWVGLFTSSGVPAPIQMKMRELIAKVAND
                     PSFKAALEKVQVVPDYRDAPEFKKFFDADYQRMAGAIKAIGKL"
     gene            1146..2252
                     /locus_tag="AC16CX_04530"
     CDS             1146..2252
                     /locus_tag="AC16CX_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3011814.1"
                     /translation="MKIKRIEPIGISIPMVKPVKMSFETVTAANNVLVRLETGDGVVG
                     WGEAGSAPAMTGETLEGMVAAVRYLAPRFEGMDVDDIGAAMGRAGRYLYGNLAAKSAI
                     EMALHDALGRARGKPACELLGGKRRGRAPLLRLVGTGKGTAADVEDALKCKAEGYVAF
                     KVKVGVGDPFEDAARTRKVCEALGNGDLLVCADANQGWSAEQAVEYVRAVADTRLAFF
                     EQPVPVEDLEGMAKVARASRVPIGADEGLHSMEDLRRHHAAGVQGGSLKTIKLGGMKP
                     VMDAARLCQSLGMKVNLACKMAESGIAAAAMSHIAAAVPAIDWGLSLTNTYLTDDLIR
                     RPLSLANGHVELPSGPGLGVEVDEAKARRYAREI"
     gene            complement(2386..3282)
                     /locus_tag="AC16CX_04535"
     CDS             complement(2386..3282)
                     /locus_tag="AC16CX_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011815.1"
                     /translation="MTTVASATIAYKVPQRRHPVLLFLTQQPLGAAGLVVIVLMAVCA
                     AFAQWVAPYDPLTVDYAAQLARPSAEHWLGTDSFGRDVLSRIIYGARTALAIGFIASF
                     VGCTAGAVVGVVSAYFGGKTDLIIQGVMDVLLSFPIIVLAITVVAVLGNNVVLGIDIN
                     LIIAISIPMLPRVERVVRASALAIRELPYVDAARAAGYSHSRIIFRHMVPNVVAPYLI
                     LLTAFVAQAILAEASLSFLGLGVTEPTPSWGLMLSGAAADFYQQAPWMIVFPGIAISL
                     GVFAFNLFGDSLRDWLDPKIKL"
     gene            complement(3396..>3956)
                     /locus_tag="AC16CX_04540"
     CDS             complement(3396..>3956)
                     /locus_tag="AC16CX_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3011816.1"
                     /translation="DQSIRVFSIAGLAVPSFWLGMIIILLLLTMFSWIPPLTFTPFLE
                     NPWENLSQLIWPALAVGYRYSAVSTRMMRSSILEVLQEDYIRTARAKGVIERLVVARH
                     AMRNALLPVVTVIGLEFAFLIGGLVVTEQVFNLNGIGKLFVQAVARSDFTMIQALVLV
                     VSVFFVLVNFVMDLMYAFLDPRIRYH"
BASE COUNT          781 a         1279 c         1295 g          601 t
ORIGIN      
        1 gccccggcgt cggttaactt tccgcgagca ggacccgagc agccgtgtgg gaccgcatcc
       61 ataggaggag ggaaaccatg atcacccgat tgacggcagc actcgcgcta ttgttggccg
      121 cagcgtacgg ctacgcccag gattacccaa cgcgcgcgat cacgatcgtc gtgccgaacc
      181 cgcccggcgg gatgaaccag atccacgccc agccgctgtc cgcggtggtc gagaagctca
      241 ccagccagcc cgcaccggtc gtcaaccgcc ccggcgggac cgcggctgtc ggtaccgctt
      301 ccgtggtgaa cgcgccgccc gacggctaca gcatcctggt gacgacgccg aacctctatc
      361 tggtgatcga gaaggacaag ctttacggca tcaagtcgcc cttttcgctc gaccagatcg
      421 cactgctcgc tctgctctcc gccgatccgc tgatcatggt cgtgcaccct tcattgccgg
      481 tgaagaccgc caagcagctc gttgcgctcg ccaagtcgaa gccgaacgaa ctcgtcttct
      541 cgacttccgg cccgtacggc atcacgcata ccccccaagc gctgttcatg gaagcgacca
      601 agaccaagat gcggcacctg ccgaccacag gcggcggccc ggcgatcacc caagcgctgg
      661 gcgggcacgc ccaggtgacg ggcggcggac cggcggcgat ctacgcgtat gtcaagtcgg
      721 gcaagctgcg cccgatcgcg agctggggca ccaagccgca tccgtcgatg ccggaggtgc
      781 cgacgttcaa gtcccagggc atcgacatcg aggcgtacct gtgggtcgga cttttcacct
      841 cttcgggcgt tccggcgccg atccagatga agatgcgcga gctgatcgcc aaggtggcga
      901 acgacccgag cttcaaggcg gcgctcgaga aggtccaggt cgtgccggac taccgggacg
      961 cgcccgagtt caagaagttc ttcgacgcag actaccagcg gatggcgggc gcgatcaagg
     1021 ccatcggcaa gctctgagcg cgccgccggc gcgcggtgat gggtgatggg tgatgagttg
     1081 aaattcaatc tcgttcatcc ggcacgtatt gctcattacc cattacccat taccggtttg
     1141 cttgtatgaa gatcaagcgc atcgagccca tcgggatcag catcccgatg gtgaagccgg
     1201 tcaagatgtc gttcgagacg gtgaccgccg cgaacaacgt gctggtgcgg ctcgaaaccg
     1261 gcgacggcgt cgtcggatgg ggggaggccg gctccgcgcc ggccatgacc ggcgagacgc
     1321 tcgagggcat ggtggccgcg gtgcgctatc tcgcgccacg gttcgagggg atggacgtgg
     1381 acgacatcgg cgcggccatg gggcgagccg gccgttatct ttacggcaac ctcgccgcaa
     1441 agtcggcgat cgagatggcg ctgcacgatg cgctggggag ggcgcgcggc aagcccgcgt
     1501 gcgagctgct tggcggcaag cggcgcggcc gcgccccgct gctgcggctc gtcggcaccg
     1561 gcaagggcac cgccgccgac gtcgaggatg cgctgaaatg caaggcggaa ggctacgtcg
     1621 cgttcaaggt caaggtcggc gtcggcgatc ctttcgagga cgcagcccgc acgcgcaagg
     1681 tgtgcgaggc gctcggcaac ggagacctcc tcgtttgcgc cgacgccaat cagggttgga
     1741 gcgcggagca ggccgtcgag tacgtgcgcg cggtggcgga tacccgcctt gccttcttcg
     1801 agcagccggt gccggtggag gacctcgagg gcatggcgaa ggtcgcgcgc gcgagccgtg
     1861 tcccgatcgg cgccgacgaa ggattgcact cgatggagga cttgaggcgc caccacgcgg
     1921 ccggcgtcca ggggggcagc ctcaagacca tcaagctcgg cggcatgaag ccggtgatgg
     1981 acgcggcgcg cctctgccaa agcctgggaa tgaaggtcaa tctcgcctgc aagatggccg
     2041 agtccggcat cgcggctgcc gccatgtccc acatcgcggc ggcggtgccg gcgatcgact
     2101 ggggcctgag cctgaccaac acctacctga cggacgacct gatccgacgg ccgttgagcc
     2161 tcgcgaacgg ccacgtcgag ctaccgtccg gccccggtct cggcgtcgag gtggacgagg
     2221 cgaaggcccg ccgctacgcc cgcgaaatat agtgactggg tgattaggtg attaggtaat
     2281 tgagtgagac ctcggccagg aaatcgcgat taggcaggct cagccactca atcactcagt
     2341 cacttaatca cttagtcact gatcaagctg tgacgcgctt gcccgtcaca gcttgatctt
     2401 gggatcgagc cagtcccgca aggaatcgcc gaagaggttg aaggcgaaca cgcccaggct
     2461 gatcgcgatg ccggggaaca cgatcatcca cggcgcctgc tggtagaagt ccgccgccgc
     2521 ccccgagagc atcagccccc acgacggcgt cggctccgtc acgcccagcc ccaggaacga
     2581 gagcgaggct tctgcgagga tcgcctgcgc cacgaatgcc gtcagcaaga tcaggtacgg
     2641 cgcgaccaca ttgggaacca tatggcggaa gatgatgcgc gagtgcgaat agcccgctgc
     2701 gcgcgcggcg tccacgtacg gcagctcccg gatcgccagc gcggaggcgc gcaccacgcg
     2761 ctcaacccgc ggcagcatcg ggatgctgat cgcgatgatg agattgatgt cgatgccgag
     2821 caccacgttg ttgccgagca ccgcgaccac ggtgatcgcc agcacgatga tcgggaacga
     2881 cagcagcacg tccatcacgc cctggatgat gaggtcggtc ttgccgccga agtacgccga
     2941 caccactccc acgaccgcgc cggcggtgca tcccacgaag gaagcgatga agccgatcgc
     3001 gagcgccgtg cgcgcgccat agatgatgcg cgagagcacg tcgcgcccga acgagtcggt
     3061 gccgagccag tgctcggccg acgggcgcgc cagctgtgcg gcatagtcga ccgtcagcgg
     3121 gtcgtacggc gccacccatt gcgcgaacgc cgcgcacacc gccatcagga cgataacgac
     3181 gagccccgcc gcgcccagcg gctgctgcgt caggaacagc agcaccgggt gccgccgttg
     3241 cggcaccttg taggcaatgg tggcggaagc aacggtagtc atgtctgcac gcacttgggt
     3301 gattaagtga ttaggtgatt aagtgattaa gcaactgcgc caccggccag gtattcacta
     3361 aatcacccaa tcacttagtc acttaatcac tcgcatcagt gatagcgtat gcgggggtcc
     3421 aaaaaggcgt acatcagatc catcacgaaa ttcaccagca cgaagaacac cgacaccacc
     3481 aacaccagcg cctggatcat ggtgaaatcg ctgcgcgcca ccgcctgcac gaagagcttg
     3541 ccgatgccgt tcaggttgaa gacctgctcg gtcacgacga ggcctccgat gaggaacgcg
     3601 aactcgaggc cgatcaccgt caccaccggc agcagcgcgt tgcgcatcgc atggcgcgcc
     3661 accaccaggc gctcgatcac gcctttcgcg cgcgcggtgc ggatgtagtc ctcctgcagc
     3721 acctcgagga tcgaggagcg catcatgcgc gtggacacgg cggaataacg gtagccgacc
     3781 gcgagcgcgg gccagatcag ctgcgagagg ttttcccaag ggttctcgag gaacggcgtg
     3841 aaggtgagcg gcgggatcca cgagaacatc gtcagcagca gcaggatgat gatcatcccc
     3901 agccagaacg acggcaccgc gagccccgcg atcgagaaca cgcggatcga ctggtc
//
LOCUS       DBNGWZ010000153        20852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2407807, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000153 DBNGWZ010000000
VERSION     DBNGWZ010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 20852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20852
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2407807"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="AC16CX_04545"
     CDS             <1..908
                     /locus_tag="AC16CX_04545"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3011817.1"
                     /translation="VILPKGCDEIGVAGAVQGSPVRLVKARTVDAYALADAEYVLEGV
                     LHPRDKRYETGEAEKAGVQGKTFFHPEWAGYMGKAYKAPTFHVSAITMRKPESRPIIF
                     PLGVHTMDDNNIDTTVREAAIFELCNRLQPGIVQDVHIPYCMTDWGGCIIQVKKRNRI
                     EEGWQRNFLSAILSCSQGMRLAIAVSEDVDIYSMDDIMWCLTTRVNPKTDILNPIPGG
                     RGQTFMPAERMTAGEKAWTASNTLFEGGMGIDATVPYGYEKDFHRPVYPVDRVKPEDF
                     FDARDIARMKARMEGWVLSLARTGR"
     gene            920..2119
                     /locus_tag="AC16CX_04550"
     CDS             920..2119
                     /locus_tag="AC16CX_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3011818.1"
                     /translation="MPAPGAAMLDGLRTLDCSDRLGWLAGRMLADLGAEVVKVEPPDA
                     ELGAADWQAYNANKRLLRLDSRRPRERAALVDLLAHADVLIETTAPGSPQAELFRPDA
                     LRARNPRLIQVSIRPFGADGPRAHWRASDLELMAAGGAMSLAGEPEGAPMRVSMPQAC
                     LWAGAQAAVGALVALAHRGATGAGQQVAVSAQAAVLTALAHAPTFYDLLGEVPTRCGE
                     YITGRTLTGARMRAFWPCRDGYINFVLYGGVAGRRSNQQLASWMRESAAPLGALAGID
                     WATFDPKPLTQAEVDALEQPILRFFAGLTKREFLEGAAAREMLGYPVSTVEDIAADPQ
                     LEARGFWDDLQCSTGGRARHCGSFAMVDGKRAPLRYAPGEPVDLEVLIAEFRGKCVRA
                     PVAASAP"
     gene            2116..3336
                     /locus_tag="AC16CX_04555"
     CDS             2116..3336
                     /locus_tag="AC16CX_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011819.1"
                     /translation="MSTVAQALAGVKVAEFGAYAAGPHVGKLLANFGAHVVHIESRRR
                     PDGFRLEYPPFKDGVPGLDRGGCFAFYNDSKYGVTLDLHTQAGHELARRLLAWSDIVI
                     ENMRPGVMRRLGLDYDAARSLNPGVVMLSSCNMGQTGPRANTPGFGSQLSSLAGFCGL
                     TGTPDGPPMLLYGPYIDFIASTMGAAAVLAGLDRRRRTGRGAWIDLSQYETGLHFIAG
                     ALLDYHVHGAVATRKGNRDDEAAPHEVYRCDGGRWLALSCWSDAEFGRLARAIGRHDM
                     AADPRFATRAQRQAHCAELDAEIAAWCAAQDADRATALLQEARVHAHPVNTISDLFHD
                     PQLVHRHVWRRRHHGVMGERAYCFPAFDLSDTPGDITSAAPLLGADNETVFKGFLGLS
                     AAEYAACQAQGAFQ"
     gene            3496..3957
                     /locus_tag="AC16CX_04560"
     CDS             3496..3957
                     /locus_tag="AC16CX_04560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3011820.1"
                     /translation="MIDRPSPAPPRVRPVRVEDLDQVIAIDADTTGLRKVDYWYELFH
                     RYGTRGLRQRFFLVVESDDAIKGFVVGEVRDWEFGSPPCGWVFGISVRSDARLAGVGT
                     RLLEAICQCFRRAGVNKVRTMLARDNHLVMSFFRSQGMMAAPFIALERDLD"
     gene            3971..4465
                     /locus_tag="AC16CX_04565"
     CDS             3971..4465
                     /locus_tag="AC16CX_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HIR3011821.1"
                     /translation="MKLQKSTSIALCSVLEAASDPARQISAADIASKFGVSPHHLGKV
                     LRQLGRAGIVESARGVGGGYRFSGNAKRLTLMDVIDLFESISALPAESGGKEYTTDVG
                     RAIARVLSEIDETASSTFRSISIDTMLKLIERDRRAREPRPQRQTSDSGSPGWLPGSK
                     PPTS"
     gene            complement(4405..6012)
                     /locus_tag="AC16CX_04570"
     CDS             complement(4405..6012)
                     /locus_tag="AC16CX_04570"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3011822.1"
                     /translation="MSRKTQRPAAAGQARKSGPIQDLRDWLARVDELGELVRVAKPVD
                     RDEEMSAIAYLVAKQQPSPAVLFERARGFEKSPFGAKLLWNILGPSLKRIALTLEEPP
                     ETPAVELIRRVKDKLKQRIPPREVARGEAPVYEHTLTGDQIDLDQLPIPRHWPLDGGR
                     YAGTGDAVITRDPDSGYLNVGTYRMMQQSRNQVGLYLSPGKDARLHITRAWQRGDPIY
                     VAAAWGIDPLYMLVGSQTFPKNVSEYEYAGGIRGEPVPVVRGTTTDLLLPANAEFVIE
                     GVIRPDSVKAEGPFGEFPGYYGRPEAGAPLVEVTAVHHRSNPILTNALMADYPSCEQS
                     GFFSIIRSAKIWDDLDKLGVPGVQGVYSHPAAAGGFGMTVLSLEQRYAGHAAQALALA
                     AQVPGGAYYTKWIIAVDEDVDPTDMDQVIWAMASRCNPIDDIDILRNTWSTWLDPTQN
                     PPEKRPYGSKALINACKEHRHLPVFSKRTTLRRDIYETVARRWKELGLPGQIPEVRAF
                     EAETKVMYHEVGGFEPGSQPGEPESEV"
     gene            6225..6881
                     /locus_tag="AC16CX_04575"
     CDS             6225..6881
                     /locus_tag="AC16CX_04575"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3011823.1"
                     /translation="MPASVKSKVEDAVLVTRRRSQILGAAIELFGKRGYYATTIREIA
                     ERAGVSTGLVYQYFGDKEDVLFLALQTVLDAYRERIPRATEGIRDPLMRFRAAVHAYC
                     RVIDANIDATVLAYRETKSVSRARRNIIKQKETETNALIEAHVRDCVKAGLFEKVDAE
                     LLTYQIVMFAHAWALKAWHYRGKMTVDQYVDRNLALLLHPLLTERGRRENLHLVAASH
                     "
     gene            complement(6892..7746)
                     /locus_tag="AC16CX_04580"
     CDS             complement(6892..7746)
                     /locus_tag="AC16CX_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HIR3011824.1"
                     /translation="MKTQDLFTPANVDFRNFQPKHFGWQLDGKVATVTLNRPERKNPL
                     TFESYAELRDTFRNLTYAPDVKAVVVTGAGGNFCSGGDVHEIIGPLVRMQEGGDMGGL
                     LAFCRMTGDLVKAMRGCPQPIVAAVDGICAGAGAIIAMASDLRCGTAASKVAFLFARV
                     GLAGADMGACNILPRIIGAGRAAELLYTGRSMDGAEAERWGFYNKLCEPGALLAEARA
                     LARSLADGPTFAHAMTKKCIHQEWSMGIDDAIEAEAQAQAICMQTQDFGRAYRAFVAK
                     QRPVFEGN"
     gene            complement(7758..8504)
                     /locus_tag="AC16CX_04585"
     CDS             complement(7758..8504)
                     /locus_tag="AC16CX_04585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011825.1"
                     /translation="MNMNGKTAVVTGAASGIGKATALELARAGVRVILADIDEQRGME
                     TQREAQTAGLLAEFLRVDITDAGSVSRFAGEALKRAGRVDILVNAAGWDRTQPFLENP
                     PEFWDQVMNINLMGPIRLTRQLLPPMFEARVGKIVNVASDAGRVGSLGETVYSAAKGG
                     LIAFTKSLARESARFGVTVNCVCPGPTDTPLWAAVPDKLKEAFVRAIPFRRVAKPQEI
                     ADAIFFFASPRSDYITGQVLSVSGGLTMVG"
     gene            complement(8501..9289)
                     /locus_tag="AC16CX_04590"
     CDS             complement(8501..9289)
                     /locus_tag="AC16CX_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3011826.1"
                     /translation="MLTLDLGQGVARLTLDRAPVNAINAQWVTGFHRLLDDLARHDDW
                     HVLHIRGSQKVFCAGADLAEMRGRFAAPDGVDAMVDSAAAMQHLFARIEALPQVTLAE
                     IGGAALGGGFELALACDLRMAALEAKLGLPEARLGLIPGAGGTQRLTRLAGRATATRI
                     ILTCEMADGATAEALGLVQWAVPRAELPARAARLAAEIAELPARALAACKSCISAAGD
                     PACDGFGEEIEASRRLYGDAATRERVQAFLARPARSTTTEKEQT"
     gene            9542..10759
                     /locus_tag="AC16CX_04595"
     CDS             9542..10759
                     /locus_tag="AC16CX_04595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011827.1"
                     /translation="MEASAVTREEAGRFAHLEWPFFGKAHSRLAREAADWADQAVGDW
                     VHAAEADTVCRRLVHEMGAAGWLDYAVPPGGADAARLDLRSICILREVFAFRGGLADF
                     AFAMQGLGSGAIAIAGSKALRSDYLPRVARGESIAAFALSETDSGSDVAAIAATARRD
                     GAHYVLDGSKTWISNGGIADFYVVFARTGEAPGTRGLSAFVVDAGAPGLSADERIEII
                     APHPMATLRMSGCRVPADHLLGAPGEGFKIAMRTLDIFRTSVGAAALGFARRALHEAL
                     QRARTRRLFGQMLSDLQLTQAKLADMATAIDAAALLVYRAAWTRDVAGAAITRHAAMA
                     KMYATEAAQQIVDAAVQIFGGLGVARGNPAERLYREVRALRIYEGATEVQKLIIARQL
                     LDAAAKTGESALQ"
     gene            10756..12471
                     /locus_tag="AC16CX_04600"
     CDS             10756..12471
                     /locus_tag="AC16CX_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3011828.1"
                     /translation="MSDTGHVDTFARDNLPPRDQWPDFSCELPEFRFPAQLNCAVALL
                     DDRVERGEGERIAICTSDGHCSYGELLDHANRIANVLVEDMGLIPGNRVLLPSPNGLM
                     MAACMFAIWKAGGIAVPTMALLRARELAHAIQKARISHALCDRRLAVEIEAARAHCPS
                     LSQVRYFRDDGPDSLEHLASRKSREFANVATAADDIALIAFTSGTSGIPKGAMHSHRD
                     MLAICEGFPRHVVRHTQDDIVAGTPSIAFTYGLAAVFLVPIRYGGSTVLIERHTPETL
                     LETIGRHRVTVLYSVPFMYRSMLALAGNYDLSSLRACVSAGEYLPVPTRAAWERATGL
                     KIIDGIGSTELGQIFISAAGADIRPGATGKVVPGYRAAVLDEHDRIAPPGTIGRLAVK
                     GPTGCRYLADERQKKYVVDGWNVTGDAYLLDQDGYFFYQARVDDMIVSAGYNIAGPEV
                     ENALLDHPAVAECAVIGWPDEERGQIVKAFVVLRLGYQRNDDMVAELKEHVKRIISPY
                     KYPRAVEFIDSLPRTYTGKLQRFKLRPPPAPAGIAKKDAVTNGRPRRARVKRALKPSR
                     AIVRS"
     gene            12468..12932
                     /gene="hcrC"
                     /locus_tag="AC16CX_04605"
     CDS             12468..12932
                     /gene="hcrC"
                     /locus_tag="AC16CX_04605"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit gamma"
                     /protein_id="HIR3011829.1"
                     /translation="MKRLVSLWLNGRRREDAVADNLLLIDYLRESLGLTGTKRGCDGG
                     ECGACTVLIDNEPRLACLTLATCCDGRRIETIEALSAGGRLNPLQRAFHEKLGTQCGY
                     CTPGMIMAAEALLRRNPDPGEAEIREALSGNLCRCTGYVKIIESVRAAASER"
     gene            12929..15271
                     /gene="hcrA"
                     /locus_tag="AC16CX_04610"
     CDS             12929..15271
                     /gene="hcrA"
                     /locus_tag="AC16CX_04610"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HIR3011830.1"
                     /translation="MSGPIPAASVTARVAGRRQPLLDGIEKVTGRARFTADLPAANAL
                     TGAILSSPMAHAEIARIDTSRARALPGVRAVITGEDCDVPFGILPIARNEYPLARDKV
                     RYWGEAVAAVAADDAKTARAALELIEVEYRPLPVYTSAAEARAEGAVLLHRDKPGNIE
                     REVNNSFGDVEAGFAAADLVREQNFHYSEVAHAQMEPDCALADFDPETGRLTLQSVTQ
                     VPYYVHLTVAECLKMDPASIRVVKPFVGGGFGHRTETLPFEIIAALLARAAAATVKLQ
                     LTRAQTFTIHRARPETDIRLKIGVKCSGEITAVDAEVVQRGGAIAGYGIITILYAGAL
                     MHALYRLPAARYRGFRVYTNTPPCGPMRGHGGVDMRHAFDSLLDAMAEELGLDAFAVR
                     RANLITPPYRTINDLQINSCGLPECLDWVEQASGWRARKGRLPRGRGLGLGCSHFISG
                     AAKPVHWTGEPHATVNLRLDFDGSITLLTGAADIGQGSSTLLAQIAAEALGLELARFR
                     VVSADSAITPKDNGSYSSRVSLMVGNAALAAAEELKRHLVAGAARKLEARPEEVECLG
                     ESYRVIGTERTLPYRDAVRAALETTGPITTRGTYTVPRELQGGKYRGAGVGPSPAYSY
                     AAQVVEVAVDEDTGEIKVEKIWVAHDCGFAINPLAVEGQVQGAVWFAMGQGLSEETEF
                     RNGLPMRANLLDYRVPTIVESPPIEMKIVEARDPGGPYGAKEASEGPVGGFGPALASA
                     VAEAIGIRVQALPLSPDRVLEAIHTRRRNERLKAALKDAR"
     gene            15273..16253
                     /gene="hcrB"
                     /locus_tag="AC16CX_04615"
     CDS             15273..16253
                     /gene="hcrB"
                     /locus_tag="AC16CX_04615"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit beta"
                     /protein_id="HIR3011831.1"
                     /translation="MELLPEFVVRHAETPLEAARLLAGDAGARILAGGTGLVANMRLG
                     LDAPSVLVSLDRLDGFRGVTDLGGALRLGAGVTLSRLATDADVARCAPALAAAAATVA
                     GPAHRSAATLGGNLCLDTRCVFYNQNETWRSSIGYCLKYRGDLCHVAPRGKRCHAAYS
                     GDLAPVLLVLGAEVEIAGPGGARRAPLGALYRDDGAQHLALARGEFLAAVHVPIRNRA
                     TRVAYRKARVRGAIDFPLAGVAVALEIEQGRVRDLRVALTGTNPRPLLLAGTEEFRGQ
                     ALDRALLDQLVKLVQRQASPQRTTVTSSNYRRQVAAAIARRLVAELAVPG"
     gene            16331..17800
                     /locus_tag="AC16CX_04620"
     CDS             16331..17800
                     /locus_tag="AC16CX_04620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR3011832.1"
                     /translation="MKLFEPLELRGMVISNRVMVPAMVTHLCKEDGFVTEDTIDRYVR
                     YARGGAGLIVVEAMAIHQVRSGPLLRIADDQYIPGLRRLVSRVHETSDSKIVPQIIHF
                     LKVARSGWRQTLDMLSVEEIDRIVEQFGDAVARAREAGFDGAELHCAHAYTLSSFLSR
                     TNPRTDEYTGQTLEGRLRLIGRVMTNVRRKAGDDFPVGIRFNGEEFIKNGYTVEDSKL
                     IALRLARLGFDYLSISAGGKFEDAVHNPGHVLYPYSGYSGDRCMPGDWFPRGLHVPIA
                     GEIKAFLRANGIATPVAIAGKLSDPRDAEGVLAAGRADIVGIARGLLADPDWPNKVKR
                     GELDRIVHCDYCNVCKALDGSHQTVICTLWPQGTIQAPVPDSAERAPEWTGDGGAVLE
                     AVVKEARVSLRWKKATGAAYYNVYRADGGAQPRLRDAVKVTHWVDNAVLAGTPYRYQV
                     CACTASGLAGRPTNERLIELPVTSADTQTAASPWRRPPD"
     gene            complement(17745..19274)
                     /locus_tag="AC16CX_04625"
     CDS             complement(17745..19274)
                     /locus_tag="AC16CX_04625"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3011833.1"
                     /translation="MDIVKEAPDAMSNPVGSVVSGATQRGKDPRFQFAYEDLRGWIEQ
                     AERLGEVRHVKGASWQEDIGLAADLIMHSDTAPCVIFDEVPGYPKGHRVLVNFFAGRR
                     KNMTLGLPLEFNKMELSQAFLDTYLRELPTLKHEVVKTGPVMENVMTGAEVDVTRFPV
                     PIWHERDGGRYIGTGSFNVTMDPEEKWINAGTYRVMIHDKQSVGFYISPGKHGRIHRE
                     KYRARNEPMPVVIVCGGDPLTFLMSSSEMPYGVCELDIVGGLRGKPLKVIRGKVTGLP
                     FPANAEIVLEGFVQPHKMKPEGPFGEWTGYYGSDVREEPVMDVKAIYHRNDPILFGCP
                     PQQPPEEQARYRAVTRSALIRKYLQQAGVPDVQAVWAHEVGGSRMLLGVSIKQRYPGH
                     AKQAGHVASQCHAGAYTGKYVIVVDEDIDVTNLEDLMWGMLTRSDPATSIDIINGAWS
                     TPLDPRIPPEQKERGDNTNSRAIIDACRPYHWRDKFPAVNKLSPEAYAKAMQRFGYLL
                     K"
     gene            19512..20519
                     /locus_tag="AC16CX_04630"
     CDS             19512..20519
                     /locus_tag="AC16CX_04630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3011834.1"
                     /translation="MGLLPERKVVPLRRRAKHARHAPWTAALARLDARVVELERNMAA
                     LDRRMTALGKHGRMQVAVAASLIIHAVIVFGVSFKLPDLSRLANQQPLEVTLVNARSQ
                     TRPSKADALAQANLDGGGNTDAKRRARSPLPVLREAKETADLTMAQKRAEQLEREARQ
                     LMTRARSKTAVASVPETPQPREEPQIAPDSADIMSRSLEIARLEAQIKRDWESYQQRP
                     RRRFIGSRTQEFRFARYVEDWRVKIERVGELNYPQAARDQRIYGSLVVTVSIKSDGSL
                     ERVDINRSSGQRILDAAALRIVHLAGPFQPFPADIAKDTDIISITRTWIFTRSDQFMA
                     E"
     gene            20610..>20852
                     /gene="aroE"
                     /locus_tag="AC16CX_04635"
     CDS             20610..>20852
                     /gene="aroE"
                     /locus_tag="AC16CX_04635"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434131.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3011835.1"
                     /translation="MSDKYAIIGNPVEHSLSPAIHAEFARVTGQNITYDRILAPRDGF
                     REAVLAFRDTGGKGLNVTLPFKHEAWRLAATRSGFAL"
BASE COUNT         3408 a         6984 c         7050 g         3410 t
ORIGIN      
        1 acgtgatcct gccgaagggc tgcgacgaga tcggcgtcgc cggcgccgtg cagggcagcc
       61 ccgtccggct ggtgaaggcg cgcacggtgg acgcgtacgc gctcgccgac gccgagtacg
      121 tgctcgaagg cgtgctccac ccgcgcgaca agcgctacga gaccggcgag gccgagaagg
      181 ccggcgtgca gggcaagacc ttcttccacc cggagtgggc cggctacatg ggcaaggcgt
      241 acaaggcgcc gaccttccat gtgagcgcga tcacgatgcg caagcccgag agccggccca
      301 tcatcttccc gctcggcgtg cacacgatgg atgacaacaa catcgacacc acggtgcgcg
      361 aagcggccat cttcgagctg tgcaaccggc tgcagcccgg catcgtccag gacgtgcaca
      421 tcccgtactg catgaccgac tggggtggct gcatcatcca ggtaaagaag cgcaaccgca
      481 tcgaggaagg ctggcagcgc aatttcctgt cggcgatcct gtcctgctcc cagggcatgc
      541 ggctcgcgat cgcggtctcg gaagacgtcg acatctactc gatggacgac atcatgtggt
      601 gcctgaccac gcgggtaaat ccgaagaccg acattctcaa tccgattccc ggcgggcgtg
      661 gtcagacttt catgccggcc gagcggatga cggcgggaga aaaggcgtgg accgcgtcca
      721 acacgctgtt cgagggcggc atgggcatcg atgcgacggt gccctacggc tacgagaagg
      781 attttcaccg gccggtgtat ccggtcgacc gcgtcaagcc ggaggacttc ttcgatgcgc
      841 gcgacatcgc ccggatgaaa gcacgcatgg agggctgggt gttgtcgctg gcgcgcaccg
      901 gacgctgacg cggagagcct tgccggctcc gggtgcggcg atgctcgacg ggttgcgcac
      961 gctcgactgc agcgatcggc tcggctggct cgccggccgc atgctcgccg acctcggcgc
     1021 cgaggtcgtc aaggtcgagc cgccggacgc cgagcttggc gcagccgact ggcaggcata
     1081 caacgcgaac aagcgcctgc tgcgcctcga ttcacgacgg ccgcgggagc gcgctgcgct
     1141 cgtcgacctt ctcgcgcatg ccgacgtcct gatcgagacg acggcgccgg gctcgccgca
     1201 ggccgagctg ttccgcccgg acgcgctgcg ggcgcgcaat ccccggttga tccaggtctc
     1261 gatccgaccg ttcggcgcgg atggtccgcg tgcgcattgg cgggcgagcg atctggaact
     1321 catggcagcg ggcggcgcca tgtcgctcgc gggggagccc gagggcgctc cgatgcgggt
     1381 cagtatgccg caggcgtgcc tgtgggccgg cgcacaagcc gcggtcggcg cactcgtcgc
     1441 gctggcccat cgcggcgcga ccggcgccgg gcagcaggtc gccgtgtcgg cgcaagccgc
     1501 ggtgctgacc gcgttggcgc acgcgccgac tttctacgat cttctcggcg aggtccccac
     1561 gcggtgcggc gaatacatca ccggccgcac gctgaccggc gcccgcatgc gcgcgttctg
     1621 gccgtgccgc gacggttaca tcaacttcgt actctacggc ggcgtcgccg gccgccgcag
     1681 caatcaacag ctcgcgagct ggatgcggga atcggctgcg ccgctcggcg ccctggccgg
     1741 catcgattgg gccactttcg acccgaagcc gctgacccag gctgaagtcg acgcgctcga
     1801 gcagccgatc ctgcggttct tcgccggcct gaccaagcgc gaatttctcg agggcgcagc
     1861 cgcgcgcgaa atgctcggct atccggtgtc caccgtggag gacatcgccg ccgacccgca
     1921 gctcgaagcg cgtggtttct gggacgatct gcagtgctcc accggaggac gcgcgcgcca
     1981 ctgcggcagc ttcgcgatgg tcgacggcaa gcgcgcaccg ctgcgctacg cgccggggga
     2041 gccggtggac ctcgaggtgc tgatcgctga attccgcggc aagtgcgtcc gcgcgcccgt
     2101 cgcggcgtcc gcgccatgag caccgtcgca caggcccttg cgggtgtgaa agtcgcggag
     2161 ttcggcgcct acgccgccgg accgcacgtc ggcaagctgc tcgccaactt cggggcgcat
     2221 gtggtccaca tcgagtcgcg gagacggccc gacggtttcc ggctcgagta cccgcccttc
     2281 aaagacggcg ttcccggtct cgaccgcggg ggctgctttg ccttttacaa cgattcgaaa
     2341 tacggcgtga ccctcgacct tcatacccaa gccgggcatg agctggcgcg gcggctgctc
     2401 gcctggtccg atatcgtcat cgagaacatg cgccccggcg tcatgcgccg gcttggcctc
     2461 gattacgacg cggcgcgctc gcttaatccg ggcgtcgtca tgctgtcgag ctgcaacatg
     2521 gggcaaacgg ggccgcgcgc gaacaccccg gggttcggct cgcagttatc atcgctcgcc
     2581 ggcttctgtg gactgaccgg cacgcctgac gggccgccga tgctgctcta cggtccctac
     2641 atcgacttca tcgcgtcgac catgggcgcc gcagcggtcc tcgccggact cgaccggcgc
     2701 cgccgcaccg gccgcggagc gtggatcgac ctgtcgcagt atgagaccgg gttgcatttt
     2761 atcgccgggg cgctgctcga ttaccacgtc cacggcgcgg tcgccacgcg caaagggaat
     2821 cgcgacgacg aggccgcgcc gcacgaagtc taccgctgcg acggcggccg ctggctcgct
     2881 ttgtcgtgct ggtccgacgc cgagttcggg cgcctggcgc gggcgatcgg caggcatgac
     2941 atggcggccg atccgcgctt cgcaacccgg gcgcagcgcc aggcccattg cgccgaactc
     3001 gacgccgaga tcgcggcgtg gtgcgccgcg caggacgccg atcgcgccac ggccctgctg
     3061 caggaagccc gggttcacgc ccatccggtg aataccattt ccgatctgtt ccacgacccg
     3121 cagctcgtgc accggcacgt ctggcgccgc cgccatcatg gcgtgatggg cgagcgggcc
     3181 tactgcttcc cggcgttcga tctgtcggac acgccgggcg acatcacctc cgccgctcca
     3241 ttgctgggcg ccgacaacga aaccgtgttc aagggatttc tcggattgag cgccgcggaa
     3301 tacgcggcgt gccaggcgca gggcgcgttt caatgaagcg gagcatccac ccgcgtcgac
     3361 gcggccacgc gcaccggcgc cgcgcgcggc gattgccccg gcaacagcat agtagtattg
     3421 tggtacttga atactgataa tcgatctgcc ggaagttttg ccgctgtgca gccgtcgcga
     3481 aaaacagcag aacgcttgat cgaccggcca tcgcctgccc cgccacgggt gcgcccggtc
     3541 cgggtggagg atctcgatca ggtgatcgcc atcgatgccg ataccaccgg gttgaggaag
     3601 gtcgactact ggtacgagtt gtttcaccgc tacggaacgc gcggtctgcg gcagcgtttt
     3661 ttcctcgtcg tggagtcgga tgacgcgatc aagggcttcg tcgtcggcga ggtgcgtgac
     3721 tgggaattcg gctcgccgcc gtgcggttgg gtattcggca tcagcgtgcg cagcgacgcg
     3781 cggctcgccg gcgtcggcac ccgcctgctg gaggcgatct gccagtgctt ccggcgcgcg
     3841 ggcgtgaaca aggtgcgaac catgctcgcc cgcgacaacc atctggtcat gtcgtttttc
     3901 cgcagccagg gaatgatggc tgcgccgttc atcgcgcttg agcgcgacct cgactgaccg
     3961 gcctgcccgg atgaaactgc agaagagcac cagcattgca ttgtgcagcg tcctggaagc
     4021 cgcatccgat ccggcccggc agatttcggc ggccgacatc gccagcaagt tcggcgtatc
     4081 cccccaccat ttagggaaag tgctgcggca actgggccgg gccgggatag tcgaatcggc
     4141 gcgcggcgtc ggtggcggct accggttcag cggcaacgcc aaacgcctga cgctgatgga
     4201 cgtgatcgac ctgttcgaga gcatctcggc gctgcccgcg gaaagcggcg gcaaggagta
     4261 tacgaccgat gtcgggcgcg ccatcgcgcg cgtgctctcc gagatcgacg agaccgccag
     4321 ctcgacattc cgctcgatca gcatcgacac catgttgaag ctgatcgaac gcgaccggcg
     4381 tgcgcgggag ccgcgcccgc agcgtcaaac ctccgattcc ggctcccccg gctggctgcc
     4441 gggttcgaag cctccgactt cgtgatacat gactttggtt tccgcctcaa acgcccggac
     4501 ctccggaatc tgacccggca gccccagctc cttccagcgc ctggcaaccg tctcgtagat
     4561 gtcccttctc aacgtggtcc gcttggaaaa caccggcagg tggcggtgtt ccttgcaggc
     4621 gttgatcaag gccttggacc cgtaggggcg cttctcgggg gggttctggg tcggatcgag
     4681 ccaggtgctc cacgtgttcc tcaggatgtc gatgtcgtcg atcgggttgc agcggctcgc
     4741 catcgcccag atgacctgat ccatgtcggt gggatcgacg tcctcatcca ctgcgatgat
     4801 ccacttggtg tagtacgcgc cgcccggcac ctgggcggcg agcgccagcg cctgcgctgc
     4861 atggccggcg tagcgttgct ccaggctcag caccgtcatc ccgaagccgc ccgcggccgc
     4921 cggatgcgag tacacgcctt gcacgcccgg cactccgagc ttgtccaggt cgtcccagat
     4981 cttggccgag cggatgatgg agaagaagcc gctttgctcg caggagggat agtccgccat
     5041 gagcgcgttg gtgaggatgg ggttgctgcg gtggtgcacc gcggtcacct cgaccagcgg
     5101 cgcgcccgcc tcgggccggc cgtaatagcc gggaaactcg ccgaacggcc cctcggcctt
     5161 caccgagtcc gggcggatga cgccctcgat cacgaattcc gcattcgccg gcagcagcag
     5221 atcggtggtc gttccgcgca ccaccggcac gggctcgccc cggatgccgc cggcatactc
     5281 gtattcggag acgtttttcg ggaacgtctg cgatcccacc agcatgtaca gcgggtcgat
     5341 tccccaggcg gccgcgacgt agatcgggtc gcctcgttgc caggcgcggg tgatgtgcag
     5401 ccgggcatcc ttgcccggtg aaagatagag gccgacctgg ttcctgcttt gctgcatcat
     5461 gcggtaggtg ccgacgttca ggtagccgct gtcgggatcg cgcgtgatca ccgcgtcgcc
     5521 ggtcccggcg tagcgcccgc cgtcaagcgg ccagtgccgg ggaatcggca gttgatcgag
     5581 gtcgatctgg tcgccggtga gagtgtgctc gtagaccggc gcctcgccgc gcgcgacctc
     5641 gcgcggcggg atgcgctgct tcagcttgtc cttgacgcgg cggatcagct cgacggcagg
     5701 cgtctcggga ggttcctcca gggtgagggc gatgcgcttc aagctgggac cgagaatgtt
     5761 ccagaggagc ttcgcgccga acgggctctt ctcgaatccg cgggcgcgct cgaaaagaac
     5821 ggcgggagaa ggctgctgtt tcgccaccag gtaggcaatc gcgctcatct cctcgtcgcg
     5881 gtcgaccggc ttggcgacgc gcacgagctc gcccagttca tcgacgcgcg cgagccaatc
     5941 gcgcaggtcc tggatcgggc cggattttcg tgcctgcccg gccgccgcgg ggcgttgggt
     6001 cttcctgctc atgcgcatag ttcagccatc gtgcaatcta tcccgagacc ggactgaagg
     6061 caacgactcg cccgcagccg gacgcgcgtc caatcgcacg atgcgcgttc acgttgacga
     6121 aaggcaggaa acgaatcacc attcaatcct cgagaacata ccggctgagg tcgcggcgcg
     6181 cgtgtcgcgc gagcggcatc gtggccggcg cgaaggacac cttcatgcct gcgagcgtca
     6241 agagcaaggt cgaggatgcg gttctggtga ccagacggcg cagccagata ctcggcgccg
     6301 cgatcgagct gttcggcaag cgcggctact acgccacgac gatccgcgag atcgccgagc
     6361 gcgccggcgt gagcaccggc ctggtctacc agtatttcgg ggacaaggaa gatgtccttt
     6421 tcctcgcgct gcaaaccgtg ctggacgcct accgggagcg cattccgcgg gctaccgagg
     6481 ggatccgtga cccgctgatg cggttcaggg cggccgtgca tgcctattgc cgcgtcatcg
     6541 acgccaacat cgacgcaacg gtgctcgcgt accgcgagac caagtcagtg agccgggccc
     6601 ggcgcaacat catcaaacag aaggaaaccg agacgaatgc gctgatcgag gcgcacgtgc
     6661 gcgactgcgt caaagcaggc ctgttcgaaa aggtcgacgc ggagctgctc acctaccaga
     6721 tcgtgatgtt cgcgcacgcc tgggcgctca aggcatggca ctaccgcggc aagatgacgg
     6781 tcgaccagta cgtcgaccgc aatctcgcgc tgctcctgca tccgctgctc acggagcgcg
     6841 gccggcgcga gaatctgcat cttgtcgccg cgtcgcactg agcgaatcct ctcagttacc
     6901 ttcaaacacg ggcctttgct tggcgacgaa cgcgcgatag gcgcgcccga agtcctgcgt
     6961 ctgcatgcag atcgcctggg cctgcgcttc cgcctcgatc gcgtcgtcga tccccatgct
     7021 ccactcctga tgaatgcatt tcttggtcat ggcatgggca aaggtgggac cgtcggcgag
     7081 cgagcgggcg agcgcccgtg cttccgccaa cagcgcgccc ggctcgcaca gtttgttgta
     7141 gaagccccag cgttcggctt cggcgccgtc catgctgcgg ccggtgtaga gcagctcggc
     7201 cgcgcggccc gcgccgatga tccgcggcag gatgttgcag gcgcccatat cggctcccgc
     7261 caggccgacg cgcgcgaaca ggaacgcgac cttgctcgcg gccgtgccgc agcgcaagtc
     7321 ggacgccatg gcgatgatcg caccggcgcc cgcgcatatt ccgtcgaccg ccgcgacgat
     7381 cggctgcggg cagccgcgca tcgccttgac caggtcgccg gtcatgcggc agaacgcgag
     7441 cagtccgccc atgtcgcccc cctcctgcat ccggaccagg ggtccgatga tttcgtgcac
     7501 atcgccgccc gagcagaaat tgccgccggc gccggtcacc acgacggcct tcacgtctgg
     7561 cgcgtaggtg agattgcgaa acgtgtcccg gagctccgca tacgattcga aagtcagcgg
     7621 attcttgcgc tcgggacggt tcagggtgac ggtcgccacc ttgccgtcga gctgccatcc
     7681 gaaatgcttc ggctgaaagt tgcggaaatc cacgttcgcg ggagtaaaca ggtcctgcgt
     7741 cttcatgaca tgtcctccta gccgaccatg gtgagcccgc cgctcacgct gagcacctgc
     7801 ccggtgatgt aatcggagcg cgggctggcg aaaaagaaaa ttgcatcggc gatttcctgc
     7861 ggcttggcga cacggcgaaa cgggatggcg cgcacgaacg cctccttgag cttgtcgggc
     7921 acggcggccc acagcggcgt atcggtcggc ccggggcaga cgcaattgac ggtgaccccg
     7981 aagcgcgccg actcgcgcgc gaggctcttg gtaaacgcga tgagcccgcc tttcgcggcc
     8041 gagtataccg tttcccccag gctgccgacg cgtcccgcgt cgctcgcgac gttgacaatc
     8101 ttgccgacgc gcgcctcgaa catcggcggc agcagttggc gggtcaggcg aatcggcccc
     8161 atcaggttga tattcatcac ctggtcccag aattcggggg gattctcgag aaaaggctgg
     8221 gtgcggtccc agccggccgc gttcaccagg atatcgaccc gaccggcgcg cttcagcgcc
     8281 tcgccggcga accggctgac ggatccggca tcggtgatgt cgacccgcag aaattcagcc
     8341 agcaggccgg ccgtttgcgc ttcgcgctgg gtttccatgc cgcgctgctc gtcgatgtcg
     8401 gccaggatga cccgcactcc cgctcgcgcc agctcgagcg ccgtcgcctt gccgattccg
     8461 gaggccgcgc cggtcacgac cgcggtcttg ccgttcatgt tcatgtttgc tccttttcgg
     8521 tggttgtcga gcgcgcgggg cgggcgagaa acgcctgcac ccgctcgcgc gtcgccgcat
     8581 cgccgtagag gcgccgcgaa gcctcgattt cctcgccaaa accgtcgcag gccgggtcgc
     8641 ccgctgccga gatgcatgac ttgcacgcgg cgagcgcgcg cgccggcagt tcggcaattt
     8701 cagcggccaa tcgcgcagcg cgcgccggca gctcggcgcg gggcaccgcc cattgaacca
     8761 ggccgagcgc ctcggccgtc gcgccatccg ccatctcgca ggtcagaatg atgcgagttg
     8821 ccgtcgcccg ccccgccagc cgggtaagcc gctgcgtgcc tccggcaccg gggatgagac
     8881 cgagccgcgc ctcgggcaag cccagctttg cctccaacgc cgccatgcgc agatcgcagg
     8941 cgagggccag ttcaaaaccg ccgcccagcg ccgcgccgcc gatttcagcc aaggtcacct
     9001 gcggcagcgc ctcgatgcgc gcaaacaggt gctgcattgc cgcagcgctg tcgaccatgg
     9061 cgtccacgcc gtcgggcgcg gcgaatcgcc cccgcatttc cgccagatcc gcgccggcgc
     9121 agaaaacctt ctggctgccg cggatgtgga gcacgtgcca gtcatcgtgc cgcgccagat
     9181 cgtcgagcag ccggtgaaaa cccgtcaccc actgcgcgtt gatcgcgttg accggcgcgc
     9241 gatcgagcgt cagccgagcc actccctgtc ccagatcgag ggtcagcacg ggacccctgc
     9301 ggcggcgggc gaggcggcgt cgcgcccatg cccatggttg attgaacgat cgttcattca
     9361 actaagagtg tggcgaatcc aggggcccct tgtcaaccct cacgtggggc gtccagcccg
     9421 gccagtcagt cccggcattg acatttcggg ctgattgaat caccattcaa tatgaatggc
     9481 cgttcagtcg ccggccgggc gtcgcgaccc agggcaggag gaggcgtgcg gagggcacga
     9541 catggaggca agtgcagtta cgcgagagga ggcgggacgc ttcgcccact tggagtggcc
     9601 gttcttcggc aaggcgcact cgcgcctcgc acgcgaggcg gctgactggg cagaccaggc
     9661 ggtgggcgac tgggtccacg ctgcggaggc cgatacggtc tgccgccggc tcgtgcatga
     9721 aatgggcgcg gcgggctggc tcgattacgc ggtgccgccg ggcggcgcgg acgcggcccg
     9781 gctcgatctg cgctcgatct gcattctgcg cgaggtattc gcctttcgcg gtggactggc
     9841 cgacttcgcc tttgcgatgc aaggcctggg gagcggcgcg atcgcgatcg cgggcagcaa
     9901 ggcgctgaga agtgattacc tgccgcgcgt ggccaggggc gagtcgatcg cggcgttcgc
     9961 gttgtccgag acggactccg gctccgacgt ggcggcgatc gcggccacgg cgaggcgcga
    10021 cggagcgcat tatgtcctgg acgggtcgaa gacgtggatc tccaacggcg ggatcgccga
    10081 tttctacgtg gtattcgcgc gcaccggcga ggcgcccggc actcgcggct tgtccgcatt
    10141 cgtggttgat gccggcgccc ccgggttgtc cgccgacgag cgcatcgaaa tcatcgcccc
    10201 ccacccgatg gcgacgctgc gcatgtcggg atgccgcgtg cccgcggacc acttgctcgg
    10261 cgccccgggg gagggcttca agatcgccat gcgcaccctc gatatctttc gaaccagcgt
    10321 cggcgcggcg gcactggggt ttgcgcggcg cgcactgcat gaagcgctcc agcgtgcgcg
    10381 gacgcgccgc ctgttcggac agatgctgtc cgatctgcaa ttgacgcaag cgaagctcgc
    10441 ggatatggcc acggcgatcg acgcggcggc gctgctggtc tatcgcgcgg cctggacgcg
    10501 ggacgtggcg ggcgcagcca tcacgcgcca tgccgcgatg gccaagatgt acgcgacgga
    10561 ggcggcccag cagatcgtcg acgccgcggt gcagatattc ggcggcctgg gcgtggcgcg
    10621 gggcaacccg gcggagcggt tgtatcgcga agtgcgggcg ctgcgcatct acgaaggcgc
    10681 gaccgaggtg cagaaactga tcatcgcgcg ccagttgctc gacgctgcgg cgaaaaccgg
    10741 cgaaagtgcg ctgcaatgag cgataccggc cacgtcgata cgttcgcgcg cgataatctg
    10801 ccgccgcgtg atcagtggcc ggatttcagc tgcgagctgc cggaattccg gtttccggca
    10861 caactcaact gcgcggttgc gttgctcgac gacagggtgg aacgggggga aggagaacgt
    10921 atcgccattt gcacgtcgga cgggcactgc agttacgggg agctgctcga ccatgccaac
    10981 cggatcgcga acgtgctggt cgaggacatg gggctcattc cgggcaaccg ggttttgctg
    11041 ccctcgccca atggtctcat gatggccgcg tgcatgtttg ccatctggaa ggccggcggc
    11101 atcgccgtgc ccacgatggc gctgctgcga gcacgggagc tcgcgcatgc gatccagaag
    11161 gcgcgcatca gccacgcctt gtgcgaccgg cgcctcgcgg tcgagatcga ggcagcccgc
    11221 gcgcattgcc cgtcgctttc ccaggtccgg tatttccggg atgacggtcc cgattcactc
    11281 gagcacctgg cgagccgcaa gtcccgtgaa ttcgcgaacg tcgcgacggc tgcggacgac
    11341 atcgcgctga tcgccttcac ttccggaacg agcggcatcc ccaagggcgc gatgcacagc
    11401 caccgcgaca tgctggcgat ctgcgaagga tttccgaggc acgtcgttcg acacacgcag
    11461 gacgacatcg tcgcgggaac gccctcgatc gcgttcacct acgggctggc cgccgtgttc
    11521 ctggtgccga tccgttacgg cggctcgacg gtgctcatcg agcgccacac ccccgagacg
    11581 ctgctcgaaa cgatcggccg ccaccgggtc acggtcttgt attcggtgcc cttcatgtac
    11641 cggtccatgc tggcgctcgc cgggaactac gacctgtcga gcctgcgcgc ctgcgtgtcg
    11701 gcgggcgagt acctgcccgt gcccacgcgc gctgcctggg agcgggcgac cggcttgaag
    11761 atcatcgatg gcatcggctc gaccgagctc ggccagatat tcatctcggc cgcgggggcc
    11821 gacattcgcc ccggcgcgac cggcaaagtg gttcccggtt accgcgccgc ggtgctcgac
    11881 gaacacgacc gcatcgcgcc gccgggcacg atcgggcgcc tcgcggtcaa aggccccacc
    11941 ggctgccgct atctggccga cgaacggcaa aagaagtatg tggtggacgg ctggaacgtc
    12001 acgggcgacg cctacctcct ggaccaggac ggctacttct tctaccaggc gcgcgtggac
    12061 gacatgatcg tgtcggccgg ctacaacatc gccgggccgg aggtcgagaa cgcgctgctc
    12121 gaccacccgg cggtcgcgga atgcgccgtt atcggctggc cggacgagga acgcggccag
    12181 atcgtcaagg cattcgtcgt gttgaggctc ggctatcagc gcaacgatga catggtcgcg
    12241 gagctgaagg agcacgtgaa gcgcatcatc tcgccctaca agtatccgcg tgccgtcgaa
    12301 ttcatcgatt ccctgccgcg cacctacacc ggaaaactgc aacggttcaa gctgcggcca
    12361 ccgccggcgc cggccggcat tgcgaagaaa gacgccgtga cgaacgggcg tccgcggcgt
    12421 gcacgggtca agcgcgcgct gaagccgtcg cgcgcgatcg ttcgctcatg aagcgtctcg
    12481 tttcgctatg gctcaacggc cggcgccgcg aggatgcggt cgccgacaac ctgctgctga
    12541 tcgactacct gcgcgagagc ctggggctca ccggcaccaa gcgcggctgc gacggcggtg
    12601 aatgcggggc ctgcacggtg ctgatcgata atgagccgcg gctcgcctgc ctaacgctcg
    12661 cgacgtgctg cgacggcagg cgaatcgaga ccatcgaggc gctgtcggcg ggcgggcgct
    12721 tgaaccccct ccagcgggcg tttcatgaaa agctcgggac gcagtgcggc tactgcacgc
    12781 ccggcatgat catggccgcc gaggcgcttc tgcgccgcaa tcccgatccg ggcgaagccg
    12841 agatccgcga ggcgctctcg ggcaacctgt gccgctgcac cggctacgtg aagatcatcg
    12901 agtcggtccg ggcagccgcg tccgaacgat gagcggtccg atcccggcag cgtcggtcac
    12961 ggcacgcgtc gcggggcgcc gccagccgct gcttgacggc atcgagaagg tcaccggccg
    13021 ggcacgcttc acggccgacc tgcccgcggc caacgcactt accggggcga tcctgtcgag
    13081 cccgatggcc catgccgaga tcgcgcgcat cgacacttcg cgcgcacgcg cgctgcccgg
    13141 cgtgcgcgcc gtcatcaccg gcgaggactg cgacgtgccc ttcgggatcc tgcccatagc
    13201 gcgcaacgaa tatccgctgg caagggacaa ggtgcgctac tggggcgaag cggtcgcggc
    13261 agtggcggcg gacgacgcga agacggcgcg cgcggccctt gagctgatag aggtcgagta
    13321 tcgcccgctt cccgtctaca cgtccgccgc cgaggcgcgc gcggagggcg cggtcctgct
    13381 gcaccgggac aagccgggaa acatcgagcg cgaggtcaac aactcgttcg gcgatgtcga
    13441 ggcgggcttt gcggcggccg acctggtgcg cgagcagaac tttcattatt cggaggtggc
    13501 gcacgcacag atggaaccgg actgcgcgct cgccgacttc gaccccgaga ccggccggct
    13561 gacgctgcaa tcggtgacgc aggttcccta ctacgtgcac ctgaccgtcg ccgagtgcct
    13621 caagatggat ccggcgtcga tccgcgtggt gaagccgttc gtcggcggcg gcttcggcca
    13681 ccgcacggaa acgctcccgt tcgagatcat cgcggcgctg ctcgcgcgcg ccgcggccgc
    13741 cacggtcaag ctgcagctca cccgcgcgca gacgttcacg atccaccgcg cgcggcccga
    13801 gaccgacatc cggctcaaga tcggcgtgaa atgcagcggc gagatcaccg cggtcgacgc
    13861 cgaggtcgtg cagcgcggtg gcgccattgc cgggtacggc atcatcacga tcctgtacgc
    13921 gggcgcgctc atgcacgcgc tgtatcgcct gccggcggcg cgctaccggg gctttcgcgt
    13981 ctacaccaac acgccgccct gcggtcccat gcgcggccac ggcggagtgg acatgcggca
    14041 tgcgttcgac agtctgctcg atgcgatggc cgaagaactg ggactcgacg cattcgcggt
    14101 gcggcgcgcc aatctcatca cgccgccgta ccgcacgatc aacgatttgc agatcaactc
    14161 ctgcggattg cccgagtgcc tggactgggt ggagcaggcc tcgggctggc gcgcccgcaa
    14221 gggcaggctg ccccgcgggc gcggcctggg tctcggctgt tcgcacttca tctcgggcgc
    14281 ggcgaagccg gtgcactgga cgggagaacc ccatgcaacg gtcaatctgc ggctcgactt
    14341 cgacggctcg atcaccctgt tgaccggcgc cgcggacatt ggccagggct cctcgacgtt
    14401 gctcgcgcag atcgccgccg aggcgctggg cctcgagctt gcgcgctttc gcgtcgtctc
    14461 cgcggacagc gcgattacgc cgaaggacaa cggctcgtac tcctcgcgcg tgtcgctcat
    14521 ggtcggcaac gcggcgctcg cggccgccga agagctcaaa cggcacctgg tcgccggcgc
    14581 cgccaggaag ctggaagcgc gccccgaaga ggtcgagtgc ctgggcgaga gttaccgggt
    14641 catcggcacg gagcgaacct tgccgtaccg cgacgccgtg cgtgccgcgc tcgaaaccac
    14701 cgggccgatc accacccggg gcacctacac cgtgccgcgc gaattgcagg gcggcaagta
    14761 ccgcggggcg ggcgtcggcc cgagcccggc ctactcgtac gcggcgcagg tggtggaggt
    14821 ggccgtggac gaggacaccg gcgaaatcaa ggtcgagaag atctgggtgg cgcacgattg
    14881 cggattcgcc atcaatccgc ttgccgtcga gggccaggtc cagggagcgg tgtggttcgc
    14941 catgggccag gggctcagcg aggagacgga gtttcgcaac ggcctgccga tgcgcgccaa
    15001 tcttctggac taccgcgtgc ccacgatcgt cgagtcgccg ccgatcgaaa tgaaaatcgt
    15061 ggaagcgcgc gatcccggcg ggccgtacgg cgcgaaggaa gcgagcgaag gcccggtcgg
    15121 cggcttcggc ccggcgctgg cgagcgcggt agccgaagcc atcggcatac gggtgcaggc
    15181 gctgccgctg tcgccagacc gggtgctgga ggcgatccat acccgccggc gcaacgagcg
    15241 cctgaaagcc gcgctgaagg acgcgcgttg agatggaact gctgcccgag ttcgtggtga
    15301 ggcacgcgga aacgccgctg gaagcggcgc gcctgctcgc aggcgatgcc ggcgcgcgga
    15361 tccttgccgg cggaaccggg ctggtcgcca acatgaggct gggcctggac gcgccgtcgg
    15421 tgctcgtttc actcgaccgg ctcgatgggt ttcgcggggt caccgacctg ggcggcgcgt
    15481 tgcggttggg agccggtgtc acgctttccc gcctggcgac cgatgccgac gtcgctcggt
    15541 gcgcgccggc gctcgcggcg gccgccgcga cggtcgccgg accggcgcac cgcagcgccg
    15601 ccaccctcgg aggcaacctc tgcctggaca cgcgttgcgt gttctacaac cagaacgaga
    15661 cgtggcgcag ctcgatcggc tactgcctga aatatcgcgg cgacctctgc catgtcgcgc
    15721 cgcgcggcaa gcgatgtcat gccgcataca gcggcgatct cgcgccggtg ctgctggtgc
    15781 tcggggccga ggtcgagatc gcgggaccgg gcggcgcgcg gcgtgcgccg ctcggcgcgc
    15841 tgtaccgcga tgacggcgcg cagcacctgg cgcttgcccg cggcgaattt ctcgccgccg
    15901 ttcacgtgcc gatccggaac cgtgcgacgc gcgttgcgta tcgcaaggcg cgggtgcgcg
    15961 gcgcgatcga tttcccgctc gcgggggtcg cggtcgcgct cgaaatcgag caaggccgcg
    16021 tgcgcgactt gcgggttgcg ctcaccggca ccaatccgcg cccgctgctg ctcgccggca
    16081 ccgaggaatt tcgcggtcaa gcgctcgatc gggcgctgct cgaccagctc gtgaagctgg
    16141 tgcaacgcca ggcaagcccc cagcgaacca cggtgacttc gtcgaactac cggcggcagg
    16201 ttgccgcggc gatcgccagg cgcctggtcg ccgaactcgc ggttcccggt tgatgggcgg
    16261 cggtggccat ggtcaagatc atcgtccgcc ggcgacccgg ccaacgaaga tcccgccagg
    16321 agagcggctc atgaagctct tcgaaccgct cgaactccgc ggcatggtga tttccaatcg
    16381 ggtcatggtt cccgccatgg tcactcatct gtgcaaggag gacggcttcg tcaccgagga
    16441 cacgatcgac cgatatgtgc gctacgccag gggaggcgcg gggctgatcg tggtcgaggc
    16501 gatggcgatc caccaggtgc gatcggggcc tctcctgcgc atcgcggatg accagtacat
    16561 tccaggtctg cgccggctgg tcagccgtgt tcacgagacc tccgactcga agatcgtccc
    16621 gcagatcatc cactttctga aagtggcgcg ctccggctgg cggcagactc tcgatatgct
    16681 cagcgtcgag gagatcgacc gcatcgtcga gcagtttggc gacgccgtgg cgcgcgcgag
    16741 ggaggccgga ttcgacggcg cggagctgca ttgcgcccac gcctatacgc tttcctcgtt
    16801 cctgtcgcgg accaatccgc gcaccgacga gtacaccggc cagacgctgg aagggaggct
    16861 ccgcctcatc ggccgcgtga tgacgaacgt gcgtcgcaag gcgggagacg attttccggt
    16921 cggcatccgg ttcaacggcg aggaattcat caagaacggc tacacggtcg aggactcgaa
    16981 gctgatcgcg ctgcggctgg cgcggctggg tttcgactat ctttcgattt ccgcgggcgg
    17041 caagttcgag gacgcggtgc acaatccggg acacgtgctc tatccgtact ccggctattc
    17101 cggcgatcgc tgcatgccgg gcgattggtt cccgcggggg ttgcacgtgc cgatcgcggg
    17161 ggaaatcaag gctttccttc gcgccaacgg catcgcgacg cccgtcgcca tcgccggaaa
    17221 gctctccgat ccccgggacg ccgagggtgt cctcgccgcc ggccgggccg acatcgttgg
    17281 cattgcgcgc gggctgctgg cggatcccga ctggccgaac aaggtcaagc gcggcgagct
    17341 cgaccgcatc gtgcactgcg attactgcaa cgtctgcaag gcgctcgacg gcagccacca
    17401 gacggtcata tgcacgttgt ggccacaggg cacgatccag gcgcccgtgc cggacagcgc
    17461 ggagcgcgca cctgaatgga ccggcgacgg cggggcagtg ctcgaagcgg tcgtgaagga
    17521 agcgcgcgtg tcactgcgct ggaaaaaagc gacgggagcg gcgtactaca acgtgtaccg
    17581 ggccgacggc ggcgctcaac cgcgactcag ggacgcggtc aaggtcacgc actgggtcga
    17641 caacgcggtg ctggccggaa cgccgtaccg ctaccaagtc tgcgcctgca ccgcctccgg
    17701 gctcgcgggc cggccgacga acgagcgcct gatcgagcta ccggttactt cagcagatac
    17761 ccaaaccgct gcatcgcctt ggcgtaggcc tccggactga gcttgttgac cgccgggaac
    17821 ttgtcgcgcc agtggtacgg gcggcaggcg tcgatgatcg cgcgcgagtt ggtgttatcg
    17881 ccgcgctcct tctgctccgg cgggatgcgc ggatcgagcg gcgtgctcca ggcgccgttg
    17941 atgatgtcga tcgaagtcgc cgggtccgag cgcgtcagca tcccccacat cagatcctcg
    18001 agattggtga catcgatgtc ctcatcgacc acgatgacgt acttgccggt gtacgcccca
    18061 gcgtggcatt gcgatgcgac gtggcccgcc tgtttggcgt gtcccggata gcgctgcttg
    18121 atcgacaccc cgagcagcat gcgcgagcct cccacttcat gcgcccacac tgcctgcacg
    18181 tcgggaaccc cggcctgctg caggtacttg cggatcagcg ccgagcgcgt gaccgcgcgg
    18241 taacgcgcct gttcctcggg cggctgttgc ggcgggcagc cgaacaggat cgggtcgttg
    18301 cggtgataga tcgccttcac gtccatcacc ggctcctcgc gcacgtcgct gccgtagtag
    18361 ccggtccact cgccgaacgg gccttcgggc ttcatcttgt gcggctggac gaagccttcg
    18421 aggacgattt ccgcgttggc ggggaacggc agtcccgtga ccttgccgcg tatgactttc
    18481 agcggcttgc cgcgcagccc gcccacgatg tcgagttcgc acacgccgta gggcatttcg
    18541 ctgctcgaca tgaggaaggt aagcggatcg ccgccgcaca cgatcacaac cggcatgggc
    18601 tcgttgcgcg cccggtactt ctcgcgatgg atgcgcccgt gcttgccggg cgagatgtag
    18661 aagcccaccg attgcttgtc gtggatcatg acgcggtagg tgccggcgtt gatccatttt
    18721 tcctccgggt ccatggtcac gttgaaactg ccggtgccga tgtaacggcc gccgtcgcgt
    18781 tcgtgccaga tcggcacggg aaagcgcgtc acgtcgactt ccgcgcccgt catcacgttt
    18841 tccatcaccg ggccggtttt gacgacttcg tgcttgaggg tgggcagctc gcgcaaatag
    18901 gtgtcgagga atgcctgcga gagctccatc ttgttgaact cgagcggcag gccgagggtc
    18961 atgttcttgc ggcgtcccgc gaagaaattg acgagcacgc gatgcccctt gggatagccg
    19021 ggcacctcat cgaaaatcac gcacggcgcg gtgtccgagt gcatgatcag gtccgcggcg
    19081 aggccgatgt cctcctgcca cgacgcgccc ttgacgtggc ggacctcgcc gagccgctcg
    19141 gcctgctcga tccacccgcg caggtcttcg taggcgaact gaaagcgggg atccttgccg
    19201 cgctgcgtcg cgccggacac gacgctgcca acgggattgc tcattgcgtc cggggcctct
    19261 ttgacgatat ccatttcatg ctcctcgctt cgccctcatg gcgttgccga aagctaccct
    19321 ccgggagggc gtagggattt gatccagcgc aaagatcatc tgtctttgct ggtccggcca
    19381 ccccctgaag gagtcaatga ccgctttgcc ggacggaaaa aacgcgattt cactttaact
    19441 tactcataca accatcggtt attatgagaa ttttcatgca gagcgccggc aggtaggtta
    19501 caatggggag tatgggattg ttacccgaga ggaaagtcgt gccgctgcgg cggcgcgcga
    19561 agcacgcgcg ccacgcgccg tggacggcgg cgctcgcgcg gctggacgcg cgggtcgtcg
    19621 agctcgagcg caacatggcg gcgctcgacc ggcgcatgac cgcgctcggc aagcacgggc
    19681 gcatgcaggt cgcggtggcc gcctccctca tcatccacgc cgtcatcgtg ttcggtgtca
    19741 gtttcaagct gccggacctg tcccggctcg cgaaccagca gccgctcgaa gtgacgctgg
    19801 tcaacgccag gagccagacc cggccgtcga aggccgatgc gctcgcccag gccaacctcg
    19861 acggcggcgg caataccgat gccaagcgcc gcgcccggag cccgctgccg gtactgcgtg
    19921 aagcgaagga gactgccgac ctcaccatgg cgcagaagcg cgcggaacag ctggagcgcg
    19981 aggcaaggca actcatgacc cgggcccgat cgaagacggc ggtcgcttcc gtgcccgaga
    20041 cgccccagcc cagggaggag ccgcagatcg cgccggactc ggccgacatc atgagccgca
    20101 gtctcgagat cgcgcggctg gaggcccaga tcaagcgcga ttgggagagc taccagcagc
    20161 gaccgcgacg gcgcttcatc ggctcgcgca cccaggagtt ccgcttcgcg cgctacgtcg
    20221 aggactggcg cgtcaagatc gagcgcgtgg gcgagctgaa ctacccgcag gccgcgcgtg
    20281 atcagaggat ctacggcagc ctggtggtga ccgtgtccat caagtccgac ggttcgctcg
    20341 agcgggtgga catcaaccgc tcgtccgggc agcgcatcct cgacgcggcc gcgctgcgca
    20401 tcgttcacct cgcggggccg ttccagccgt tcccggccga tatcgccaag gacaccgaca
    20461 tcatcagcat tacccggacg tggatcttca cccgctccga ccagttcatg gccgaataaa
    20521 agcacgattc gagatgcgcg tggcgagatt cgagatggac tgacctcgca tcccgtatct
    20581 cgcatcccgt atcccgcatc acgaatcaaa tgtcggacaa atacgcaatc atcggcaacc
    20641 cggtcgagca cagcctgtcg ccggcgatcc acgccgaatt cgcgcgcgtc acgggccaga
    20701 acatcacgta cgacaggata ctcgcgccgc gcgacggctt ccgcgaagcg gtcctggcgt
    20761 tccgcgacac gggcggcaag ggactcaatg taacgcttcc cttcaagcac gaggcctggc
    20821 gtctcgccgc cacgcgcagc ggcttcgcgc tt
//
LOCUS       DBNGWZ010000154         9899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_644360, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000154 DBNGWZ010000000
VERSION     DBNGWZ010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_644360"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..164
                     /locus_tag="AC16CX_04640"
     CDS             3..164
                     /locus_tag="AC16CX_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011836.1"
                     /translation="MTREAIQLHGAIGFTDEYDAGLYLKRALTLAAWLGHASWHRRRY
                     ARLAVGARP"
     gene            161..1330
                     /locus_tag="AC16CX_04645"
     CDS             161..1330
                     /locus_tag="AC16CX_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011837.1"
                     /translation="MNPATAAPDYNAMSDEAFRREVRAFFESEYPQHLRYILHRARWR
                     EMKDWWRKLHEKGWIAPNWPREWGGMALDAGKMVIYLEEMERHGVARPPDQGITQVGP
                     ILMRYGTESQKNHYLPRTLSGEYIWCQGYSEPNAGSDLAALQTAAVLEGDDFVVNGSK
                     IWTSLADDATHIYLLARTDRQAKRQQQGISFLLVDLKTPGITLRPIRNLAGHEEFCQV
                     FFDNARVPRGNLVGKLNDGWTVAKALLGFERLNIGSPRRPQYALTRLEMVARGKGLLD
                     DAGFLDRFIALKLDIEDGAALYGRFVEQVKRGEPLGQDVSMLKIWTMETWQRLAELLV
                     EAGAEQGVITGVHDVAGVETDFLNPFYYSRPATIYGGSSEIQRNILAKYVLKLPS"
     gene            1687..2067
                     /locus_tag="AC16CX_04650"
     CDS             1687..2067
                     /locus_tag="AC16CX_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011838.1"
                     /translation="MPKQIIHTEQAPKAIGTYSQAVRCGATVYLAGQIGLDPATMEVV
                     AGVGPQIHRVFQNLQAVARAAGGDLKDVAKLTVYLTDLANFALVNETMANYFQAPYPA
                     RAAVGVASLPRGAQVEIDAIMHLE"
     gene            2092..4185
                     /gene="recG"
                     /locus_tag="AC16CX_04655"
     CDS             2092..4185
                     /gene="recG"
                     /locus_tag="AC16CX_04655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3011839.1"
                     /translation="MKRENRREPVASPLTPHPSLSGLSKSTLTRLAKLGIASQFDLVL
                     HLPLRYDDETRLYSLNEAPAGQPVLVEGKIVECDIRYRPRRQLVCHIEDGSGVLTLRF
                     FNFYQSQLKQLAAGARVRAFGEIRHGFHGPEMIHPRYRLVQEGAPVALSLTPIYPTTA
                     GFAQDALRRLIARALADCDLADTLPRPVLESLELPPFRQAVQLLHNPPARVAREALQT
                     RHHPAWRRVKFDELLAQQLSMRLHRERRRAARAPALAPRGRLTRALLERLPFELTRAQ
                     RRAWREIERDLARPHPMQRLLQGDVGSGKTVVAALAALQGVENDRQVAVMAPTEILSE
                     QHYRKLAGWLAPIGVGVIWLSGSLGRKDKRAALAAVAKGEAKIAVGTHALIEEEVEFA
                     DLALAVVDEQHRFGVHQRLALRMKGSRPDPGAQLHQLMMSATPIPRTLAMSYYADLDV
                     SVIDEAPPGRTPVTTKLVSETRRAEVIRRVRDACMTGSQAYWVCPLIEESEQLQLKTA
                     LETHAALAATFPELKLGLVHGRLGREEKAQVMEAFRGGAIQLLVATTVIEVGVDVPNA
                     TLMVIEHAERMGLAQLHQLRGRVGRGAEAATCILLYQNSLTPAARERLRIIYEQRDGF
                     EIARHDLRLRGPGELLGARQSGIPMLRFADLETDLDLLDHARAAAAALLRDHPDAARA
                     HLARWLGGRHEFLKA"
     gene            4331..5221
                     /gene="ubiA"
                     /locus_tag="AC16CX_04660"
     CDS             4331..5221
                     /gene="ubiA"
                     /locus_tag="AC16CX_04660"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR3011840.1"
                     /translation="MTLKEKLDAYERLMRLDKPIGILLLLWPTLWALWLSAPGRLRVD
                     VLVIFVLGTALMRSAGCVINDFADRNFDPHVERTRDRPLAAGLVSPREALALAGVLLL
                     LAFALVLQLNRLTVYLSLFAVAITAIYPFLKRFFAFPQAGLGIAFSFGIPMAYAAQRG
                     ELPTAAWVMMIATFFWIIAYDTEYAMVDRDDDLKLGVNSSAILLGRYDVAAVMMCHAA
                     FIGIMAAFGAWQRLGLLYYAGLLLAAGLAVYQYRLVKERSREGCFRAFLNNNWMGAAV
                     FAGLLFDLLLDARFFLAPAR"
     gene            5218..6690
                     /gene="ubiD"
                     /locus_tag="AC16CX_04665"
     CDS             5218..6690
                     /gene="ubiD"
                     /locus_tag="AC16CX_04665"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HIR3011841.1"
                     /translation="MKYKDLRDFLAQLEARGELKRIAATVDPRLEMTEICDRVLKAGG
                     PALLFEKPRGHATPVLGNLFGTVKRVALGMGVDAEDPTAALRELGNLLAYLKQPEPPR
                     GWKDAWEKLPLLKQVLAMQPRTVSSAPCQEIVREGADADLGQLPVQTCWPGDAAPLIT
                     WGLTVTRGPNRSRQNLGIYRQQVIGRNKVILRWLAQRGGALDYRDHCLARPREPFPVA
                     VALGADPATMLGAVTPVPDALSEYQFAGLLRGSKTELAKCLSHDLQVPAGTEIVLEGW
                     IHPGETALEGPFGDHTGYYNEPDRFPVFTIERITSRRDPLYHSTYTGKPPDEPAVLGM
                     ALNEVFVPLLVRQFPEIVDFHLPPEGCSYRIACVSIRKQYPGHAKRVMFGIWSYLRQF
                     LYTKFIIVTDADVNVRDWKEVIWALTTRVDPRRDTLITEHTPVDTLDFASPVPGLGSK
                     MGIDATNKWPGETDRRWGEAIAMSEEVKRRVDAMWRELGL"
     gene            complement(6905..7099)
                     /locus_tag="AC16CX_04670"
     CDS             complement(6905..7099)
                     /locus_tag="AC16CX_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011842.1"
                     /translation="MDLSPVVSAPKAGWQFSGRVGPLRWLSPLDGEGDTQLRLGGRVP
                     GQPRMPGMGLFNVGVHYAFE"
     gene            complement(7541..9031)
                     /gene="dacB"
                     /locus_tag="AC16CX_04675"
     CDS             complement(7541..9031)
                     /gene="dacB"
                     /locus_tag="AC16CX_04675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3011843.1"
                     /translation="MTRGIAPSRDAGRAGRLKRWLLAASALLAAWLPPAASAQLPEPV
                     ARALAQAGIPESAAAFYVHEIGAGRPLIAEGAERAMNPASTIKLITTYAALELLGPAY
                     LWTTEAYAAGELKDGVLAGDLVIKGRGDPKLTIENFWLLLRSLRGRGLREVRGDLVLD
                     RGYFAAADFDPARFDDQPTRPYNTGPDALLVNFKAVRLQFLPEADTRSVSILAEPALP
                     QVQIVNNLVLDNGPCGDWVSKLKLDAQGGATSARLAFNGRYAASCGERVRNYSVLGHS
                     QYVLALFRELWRELGGTFAGSVRDGAAESDARLIAAVQSPTLSEVVRDINKFSNNVMA
                     RQLFMTLGAAGAGAPATAEKTGRVIRQWLAGKGLPFPELVLENGSGLSRLERVSARSL
                     GELLLAAFKSPVMPELIASLPLTAVDGTMKKRLAGADVAGQAHIKTGSLAGVRSIAGY
                     VLDAQGRRVAVVFIVNHANAGNAQPVQDALLQWIYSRDADGCCQRR"
     gene            complement(9223..>9899)
                     /locus_tag="AC16CX_04680"
     CDS             complement(9223..>9899)
                     /locus_tag="AC16CX_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3011844.1"
                     /translation="IVEPLVQGACGMAMHDAEYLRRARAICDRYGVHFIADEIMTGFG
                     RTGSFFAHEQAGITPDFLCLSKGITGGYLPLSAVMTRDAIYQAFYDDDVTRAFLHSHS
                     YTGNALACRAALATLDIFEQDRVIEANRATGAQLTSAASAIARHPRVKHFRSCGMILA
                     FDVETADPGFARRFSASALERELLLRPLGNTVYFMPPYVIGRDEIELLVRRTGEVLDA
                     LGVCRT"
BASE COUNT         1657 a         3264 c         3343 g         1635 t
ORIGIN      
        1 gaatcacgcg cgaggcgatc cagctccacg gcgcgatcgg cttcaccgac gaatacgacg
       61 cggggctata tctcaagcgc gcattgacgc tcgcagcgtg gctcggacac gcaagctggc
      121 accgccgccg ctacgcgcgg ctcgccgtcg gagcccggcc atgaaccccg cgacggcggc
      181 acctgactac aacgcgatga gcgacgaggc cttccgccgg gaagtccgcg cgttcttcga
      241 gtccgaatat ccgcagcacc tgcgctatat cctgcaccgc gcgcgctggc gcgagatgaa
      301 ggactggtgg cgcaagctcc atgaaaaggg ctggatcgcg cccaactggc cgcgcgagtg
      361 gggcggcatg gcgctcgatg ccggcaagat ggtgatctac ctcgaggaaa tggagcgcca
      421 tggcgtcgcc cgccctccgg accagggcat cacgcaggtc ggccccatcc tcatgcgcta
      481 cggcaccgag tcgcagaaga accattatct cccgcgcacg ctctctggcg agtacatctg
      541 gtgccagggc tattccgagc ccaacgcagg ctctgatctc gcggccctgc aaaccgccgc
      601 ggtgctcgag ggagacgatt tcgtcgtcaa cggcagcaag atctggacct cgcttgccga
      661 cgacgcgacc cacatctatc tgctcgcgcg caccgaccgg caggcgaagc ggcagcagca
      721 gggcatcagc ttcctgctgg tggatctgaa gaccccgggc atcacgctgc gcccgatccg
      781 caacctcgcg ggccacgagg agttctgcca ggtgttcttc gacaacgcgc gtgtgccgcg
      841 cgggaacctg gtgggcaagt tgaacgacgg ctggaccgtg gcgaaggcgc tgctcggctt
      901 cgagcggctc aacatcggca gcccgcgccg cccgcagtac gcgctcaccc ggctggaaat
      961 ggtcgcgcgc gggaaagggc tgctcgacga tgccggcttc ctcgaccggt tcatcgcgct
     1021 caagctcgac atcgaggacg gcgcggcgct ctacggccgc ttcgtcgagc aggtgaagcg
     1081 cggtgagccg ctcgggcagg acgtgtccat gctcaagatc tggaccatgg aaacgtggca
     1141 gcggctggcg gagctgctgg tcgaagccgg cgccgagcag ggcgtcatca ccggcgtgca
     1201 cgacgtcgcc ggcgtggaga ccgatttcct gaatcccttc tactactcgc gccccgccac
     1261 gatctacggc ggctcgagcg agatccagcg caacatcctc gccaagtacg tgctgaaact
     1321 gccaagctga cacggccgcc cccattggtt ctttcggaaa tggttgacgc tccatttccg
     1381 ccatcctttt cctctcttct ctcgggagag ggtgcgccga aacgttgagc aggggccctg
     1441 gtttccgggg atgcgaccgt gcaggcgcac ataagcacgc gtttagtcgc ggcgctgcat
     1501 cccgctccgg agtcgcatcc aagcataatg cgcttggaac ctgctccgcc ctccgcgggc
     1561 gcaggggtga ggggtcgagc gtcgtcaagc aattatcggg cgctcggccg cgcgctcgat
     1621 tgaaatcccg cgcgcggcgc ttcatgatac gtgagagctt cggttccgga acgaggagag
     1681 aaagccatgc ccaagcaaat cattcatacc gaacaggcgc ccaaggcgat cggaacctat
     1741 tcgcaggcgg tgcgctgcgg cgccacggtc tacctcgccg gtcagatcgg gctcgatccc
     1801 gcgacgatgg aggtggtcgc cggcgtcggc ccgcaaatcc accgcgtgtt ccagaacctg
     1861 caggcggtgg cgcgggcggc gggtggcgac ttgaaggacg tcgccaagct cacggtctat
     1921 ctcaccgatc tcgccaactt cgcgctggtg aacgagacca tggcgaatta tttccaggcg
     1981 ccctaccccg cgcgcgccgc ggttggcgtc gcgagcctgc cgcgcggggc gcaagtggag
     2041 atcgacgcca tcatgcacct cgagtgagga gtgaagagtg aggagtgagg agtgaagagg
     2101 gaaaatcggc gagagccggt cgcctcaccc ctcacccctc acccctcact gtcggggttg
     2161 agcaagtcaa ccctgacgcg gctggccaag ctggggatcg cgagccagtt cgacctggtt
     2221 ctccacctgc cgctgcgcta cgacgacgag acgcggcttt actccctcaa cgaggcgccg
     2281 gcgggccagc cggtgctggt cgagggcaag atcgtcgagt gcgacatccg ctaccgcccc
     2341 cggcgccagc tcgtctgcca catcgaggac ggcagcggcg tgctgacgct gcgcttcttc
     2401 aacttctatc agagccagtt gaagcagctc gccgccggcg cccgcgtgcg cgctttcggc
     2461 gagatccggc acggtttcca cggcccggag atgatccacc cgcgctaccg cctggtgcag
     2521 gagggcgcgc cggtcgcgct ctctctcacg ccgatctacc cgaccaccgc gggattcgcg
     2581 caggacgcgt tgcgccgtct gattgcgcgg gcgcttgccg actgcgacct cgcggacacg
     2641 ctgccgcggc cggtgctgga aagcctggag ctgccgccgt tccggcaagc ggtgcagctg
     2701 ctccacaatc cgcctgcgcg cgttgcgcgg gaggcgttgc agacgcggca tcacccggcg
     2761 tggcgcaggg tcaagttcga cgagctgctg gcgcagcagc tctcgatgcg gctgcaccgg
     2821 gagcggcgcc gcgccgcccg cgcgccggcg ctggcgccgc gcggcaggct cacgcgcgcg
     2881 ctgctcgagc gcctgccctt cgagctcacg cgcgcgcagc gcagagcctg gcgcgagatc
     2941 gagcgcgacc tcgcccggcc ccacccgatg caacgcctgt tgcagggcga tgtcggcagc
     3001 ggcaagaccg tggtggcggc gctcgcggcg ctgcagggcg tggagaacga ccgccaggtg
     3061 gcggtgatgg cgcccacgga aatcctctcc gagcagcatt accgcaagct cgccggctgg
     3121 ctcgccccga tcggggtcgg cgtgatatgg ctctcgggca gcctcggccg caaggacaaa
     3181 cgcgcggcgc tcgccgctgt tgcgaagggg gaagcgaaga tcgcggtggg cacccatgcg
     3241 ctgatcgagg aggaggtcga gttcgccgac ctcgcgctgg cggtcgtcga cgagcagcac
     3301 cgcttcggcg tgcaccagcg gctggcgctg cgcatgaagg gctcgcgtcc tgacccgggc
     3361 gcgcaactgc atcagctgat gatgagcgcc acgcccatcc cgcgcacgct cgcgatgagc
     3421 tattacgccg acctcgacgt gtcggtgatc gatgaggccc cgcccggacg cactccggtc
     3481 acgaccaagc tcgtatcgga aacgcggcgc gcggaggtga tccggcgcgt gcgcgatgcc
     3541 tgcatgacgg gcagccaggc ctactgggtg tgtccgttga ttgaagagtc agaacaactg
     3601 cagttgaaga ccgcgctcga aacgcacgcc gcgctcgccg cgacctttcc cgaactgaag
     3661 ctcggcctgg tccacgggcg cctgggccgc gaggagaagg cgcaggtgat ggaggcgttc
     3721 cgcggcggag cgatccagct gctggtcgcc accaccgtga tcgaggtcgg cgtggacgtg
     3781 ccgaacgcga ccctgatggt gatcgaacac gccgagcgca tggggctcgc gcagctgcac
     3841 cagctgcgcg ggcgcgtcgg ccgcggcgcg gaagcagcca cctgcatcct gctctaccag
     3901 aattcgctca cgcccgccgc gcgcgagcgg ttgagaatca tctacgagca gcgggacggc
     3961 ttcgaaatcg cgcgccacga cctgcggctg cgcgggccgg gggagctgct cggcgcgcgc
     4021 cagagcggaa taccgatgct gcgcttcgcc gatctcgaga ccgacctcga tctgctcgat
     4081 cacgcgcgcg cggcggccgc cgccctgctg cgcgaccacc ccgatgcagc gcgcgcgcat
     4141 ctcgcgcgct ggctcggcgg gcgccatgaa ttcctgaagg cgtgattcgt gagtggtggg
     4201 tcgtgagtcg aaaaggttgg cgaaaccgga ctcgcgggtt cagtcgaatc acgactcacg
     4261 aatcacgatt cacggtttat cctaatcgcc tatactggtg gcccgctgcc tcgcgggaaa
     4321 tgccgggacc atgacgctca aggaaaagct cgatgcctac gagcggctga tgcggcttga
     4381 caagccgatc ggcatcctgc tgctgctgtg gcccacgctg tgggcgctgt ggctgtcggc
     4441 gccgggcagg ctgcgtgtcg acgtgctggt cattttcgtg ctgggaacgg cgctgatgcg
     4501 ctccgccggc tgcgtgatca acgatttcgc cgaccgcaat ttcgatccgc acgtcgagcg
     4561 cacccgggac cgcccgctgg ccgcgggcct ggtgagcccg cgcgaagcgc ttgcgctcgc
     4621 gggagtattg ctgctgctcg cattcgcact ggtgctgcag ctcaatcggc tcacggtcta
     4681 cctgtcgttg ttcgcggtcg cgatcacggc gatttatccg ttcctcaagc gcttcttcgc
     4741 ctttccgcag gccgggctcg gcattgcatt cagcttcggc atcccgatgg cctatgccgc
     4801 ccagaggggc gagctgccga cggcggcgtg ggtgatgatg atcgccacct ttttctggat
     4861 catcgcctac gacaccgagt acgccatggt ggaccgcgac gacgatctca agctcggggt
     4921 gaactcctcg gcaatcctgc tcgggcgcta cgacgtcgcg gccgtcatga tgtgccacgc
     4981 cgcgttcatc ggcatcatgg cggcgttcgg cgcgtggcag cggctcggtc tcctgtatta
     5041 cgccgggttg ctgctcgcgg cggggcttgc ggtttatcag taccgtctcg tcaaggagcg
     5101 cagccgggag ggctgtttcc gggcttttct caacaacaac tggatgggcg cggcggtctt
     5161 cgccggcctg ctattcgacc tgctgttgga cgcccgcttt tttctggcac ctgcgcgatg
     5221 aaatacaagg atcttcgcga tttcctggcc cagctcgaag cgcgtggcga gctgaagcgc
     5281 atcgccgcga cagtcgatcc ccgcctcgaa atgaccgaga tctgcgaccg cgtgctcaag
     5341 gccggcgggc cggcgctgct gttcgagaaa cccaggggac atgcaacgcc cgtgctcggc
     5401 aacctgttcg gcacggtgaa gcgcgtcgcg ctcggcatgg gggtggacgc agaggatccc
     5461 actgcggcgc tgcgcgaact cggcaatctg ctggcctatc tcaaacagcc ggagccgccc
     5521 aggggctgga aggacgcgtg ggagaaactg ccgctcctca agcaggttct ggcgatgcag
     5581 ccgcggacgg tctcttcagc accctgccag gagatcgtgc gggagggcgc ggacgctgat
     5641 ctcgggcaac tgccggtcca gacctgctgg cccggggacg ccgctcccct catcacctgg
     5701 gggctcaccg tgacccgcgg gcccaaccgg agccggcaga atctcggcat ctaccgccag
     5761 caggtgatcg gccggaacaa ggtcatcttg cgctggctcg cgcagcgcgg cggcgcgctc
     5821 gattaccgcg atcactgtct cgcgcgcccg cgtgagccgt tcccggtggc ggtggccctc
     5881 ggcgccgatc ccgcaaccat gctgggggcg gtgacgccgg tgccggacgc gctttccgag
     5941 taccagttcg cgggcctgct gcggggatcg aagacggagc tcgccaagtg cctgagccac
     6001 gacctgcaag tgccggctgg cacggagatc gtgctcgaag gctggattca ccccggcgag
     6061 accgcgctcg aggggccgtt cggcgaccac accggttact acaacgagcc ggatcgcttc
     6121 ccagtgttca cgatcgagcg catcacgagc cgccgcgatc cgctttacca ctcaacctac
     6181 accggcaagc cgcctgatga gccggcggtg ctgggcatgg cgctgaacga ggtgttcgtg
     6241 ccgctgctcg tccggcagtt tccggagatc gtggatttcc acctgccgcc ggaaggctgc
     6301 tcctaccgca tcgcctgcgt gagcatccgc aagcagtatc cgggacatgc gaagcgcgtg
     6361 atgttcggca tctggtctta tctccgccag tttctgtaca ccaagttcat catcgtcact
     6421 gacgccgacg tcaacgtccg cgactggaag gaggtgatct gggcgctgac gacgcgggtg
     6481 gacccgaggc gcgacaccct catcacggaa cacacgccgg tcgacacgct cgacttcgcg
     6541 agcccggtgc cggggctggg ttccaagatg gggatagacg cgaccaacaa atggccggga
     6601 gagaccgacc gccgctgggg cgaggcgatc gcgatgagcg aggaagtgaa acgccgggtg
     6661 gacgcgatgt ggcgcgagct ggggctttaa gcccgcatgt ttcgcgactg gtcgcgcata
     6721 ggggtcgttc ggccaaaccg cgcggggaac gtccgtgata tgcgggcttg atgattaagc
     6781 tctgcaagcg cgtatgcgtg ggcccttttt catcaagcag ttcgttccct ggcgttttac
     6841 gcccgatttt cgggcggtat tgaatcagga atttgccgga cgggcgtccg gcaaattcct
     6901 gagttcattc gaacgcgtaa tggacaccga cgttgaagag ccccattccc ggcattcgcg
     6961 gctggcccgg cacccggccg ccgagacgca actgggtatc cccctcgccg tcgagcgggc
     7021 tcagccagcg caacggcccg acccgccctg aaaactgcca tcccgccttg ggcgcggaaa
     7081 cgacggggga caggtccaac gggggttgag taacaacgct gctcgcggct ccgtagcgga
     7141 agtcataagg gttggaggaa gagagtgctg catccaatgc gggtccaaga cggtaaccgt
     7201 cggcatgcga ggcggcagaa ggcgcagctc tcgttgtgaa tcttaaaccg gtgctccggc
     7261 tctccgaccg tatttcggac gaggcctggg gccggggagg cgcgggctga gcttgctgct
     7321 gcggagcgct gacgatctcg ggctgcggga gaggggtggc gtcggatgcg tgcgccgcaa
     7381 tggccggcaa aaagccggct gcaatcgctg acaccagcac tgcgctatgg cgcggcatgt
     7441 gcatcccccc ggatgaattc attctacgcc tgccgttggg caaatcgggc aaatcaacgt
     7501 gcccgcaaca aaatgggaca cggggattgt gacaaagttt tcagcgacgc tggcaacagc
     7561 cgtcggcatc gcggctgtag atccattgca gcagcgcgtc ctgcacgggc tgcgcgttgc
     7621 cggcgttggc gtgattgacg atgaagacca cggccacgcg ccgtccctgc gcgtcgagca
     7681 catagccggc gatcgagcgc acgcccgcga gcgagccggt cttgatgtgc gcctggcccg
     7741 caacgtccgc gcccgccagg cgctttttca tcgtcccgtc caccgcggtg agcgggagcg
     7801 aggcgatcag ctccggcatc accgggctct tgaaggccgc gagcaggagc tcgcccaggc
     7861 tcctcgcgct gacgcgctcg aggcgcgaga ggccggagcc gttttccagc accagctccg
     7921 ggaatggcaa ccccttgccc gccagccatt ggcggatgac gcggcccgtc ttctcggcag
     7981 tagccggggc gcccgccccc gcagcgccca gcgtcatgaa cagctggcgc gccatcacgt
     8041 tgttggagaa cttgttgatg tcgcgcacga cctcggacag cgtgggcgac tgcacggcgg
     8101 cgatgaggcg tgcgtcgctt tccgccgcgc cgtcgcgcac gctcccggcg aaggttccgc
     8161 ccagctcgcg ccacagctca cggaacagcg ccagcacgta ctgggaatgg ccgagcacgc
     8221 tgtagttgcg cacgcgctcg ccgcagctcg cggcgtagcg gccgttgaac gcgaggcgcg
     8281 cggacgtcgc ccctccctga gcgtcgagct tcaacttgct cacccagtcg ccgcacggac
     8341 cgttgtcgag cacgaggtta ttgacgatct gcacctgcgg cagcgccggc tcggcgagga
     8401 tgctcaccga gcgcgtgtcg gcttccggca ggaattgcag ccgcaccgcc ttgaaattga
     8461 cgagcagggc gtcggggccg gtgttgtagg gacgggtcgg ctggtcgtcg aagcgcgcgg
     8521 ggtcgaaatc cgccgcggcg aaatagccgc ggtcgagcac aaggtcgccg cggacctcgc
     8581 gcaacccccg gccgcgcagg cttctcagca gcagccagaa gttctcgatc gtcagcttgg
     8641 gatcgccccg gcccttgatg acgaggtcgc ccgcgagcac gccgtccttg agctcgccgg
     8701 cggcgtaggc ttcagtggtc cagaggtagg ccggaccgag cagctcgagc gcggcgtagg
     8761 ttgtgatcaa cttgatggtg gaggcgggat tcatcgcgcg ctcggcgccc tccgcgatga
     8821 gcgggcgccc ggcgccgatt tcatgcacgt agaacgccgc ggcactctca ggtatgccgg
     8881 cctgcgcgag cgcgcgggcc accggttccg gcaattgcgc ggacgcggcg ggcgggagcc
     8941 atgcggcgag cagcgccgat gccgccagca gccatctctt gagacgccct gctcttccgg
     9001 cgtcccggct gggtgcaatt cctctcgtca tgtcagatgg cgacattatg ccgcaaggag
     9061 cttgccggat tcagttccgg caagctcctt gggcaaaacc gtctcaccct tcatgatagc
     9121 gcccactcga agcggtcgaa tgtgacgaaa agcgagggtg aattcgcata ttcgtcattc
     9181 tcgttttcct ggtaccggtt ttgtatcagg agtttgtcgg ggctaagtcc gacaaactcc
     9241 tagggcgtca aggacctcgc cggtacgccg gacgagcagc tcgatctcgt cccgcccgat
     9301 cacgtaaggc ggcatgaagt acacggtgtt gccaagcggg cgcagcagga gttcccgctc
     9361 gagcgcggac gcagagaagc ggcgggcgaa cccgggatcg gccgtttcca cgtcgaaggc
     9421 gagaatcatg ccgcacgaac ggaaatgttt gacgcgggga tgccgtgcga tcgcactcgc
     9481 ggcggacgtg agttgcgcgc ccgtcgcccg gtttgcctcg atgacccggt cctgctcgaa
     9541 gatatcgagc gtggcgagcg cagcgcggca ggcgagcgcg ttgccggtgt aggaatgcga
     9601 atggagaaaa gcccgcgtca cgtcgtcatc gtaaaacgcc tggtagatgg catcgcgcgt
     9661 catcaccgct gagagcggca ggtagccgcc ggtgatgccc ttggacagac acaggaaatc
     9721 cggcgtgatg ccggcctgtt cgtgcgcgaa gaagctgccg gtgcgcccga acccggtcat
     9781 gatctcgtcg gcgatgaagt gcactccata gcggtcgcag atcgcccgcg cccgacgcag
     9841 gtactccgcg tcgtgcatcg ccatgccgca tgcgccctgc accagcggct ccacgatca
//
LOCUS       DBNGWZ010000155         3127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1652868, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000155 DBNGWZ010000000
VERSION     DBNGWZ010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1652868"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /gene="glnA"
                     /locus_tag="AC16CX_04685"
     CDS             complement(<1..1490)
                     /gene="glnA"
                     /locus_tag="AC16CX_04685"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3011845.1"
                     /translation="MSSQQQRSLVQVEPGSYRVQRPASGEEVMEFVRKNDIRIIDLRF
                     IDLPGLWQHFSIGLPELKKDLFDDGIGFDGSSIRGFQEIHESDMLLKPDPGTAFVDPM
                     CEIPTLVMICDVVDPVLRQPYSRDPRYIAKKAEAYLKQTGIADTCYFGPELEFFIFDS
                     IRFEQNQHCGYYYVESAEGEWNSGRDEGAYGGGNLGYKPRYKEGYFPVPPHDTLQDIR
                     SEIMLALMDAGIQVEVHHHEVSTAGQNEIDMRFAPLTRMADNVMMYKYFCKNVARKHG
                     KVATFMPKPIFADNASGMHCHQSLWKGDKNLFFDAKGYALTSQACRWYIGGLLKHAKA
                     LMAFCAPTTNSYKRLVPGYEAPVNLAMSQRNRSAAARIPMYSDSPAAKRVEFRCPDPS
                     ANAYLAFAAMLMAGLDGVQNKIDPGAPLDKNIYDLPPEEAKGIAQVPGSLEESLRALE
                     GDHGFLKQGSVFTDDLIQTWLDYKWKKEVDPVRLRPHPWEFYLYFD"
     gene            complement(1819..>3127)
                     /locus_tag="AC16CX_04690"
     CDS             complement(1819..>3127)
                     /locus_tag="AC16CX_04690"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3011846.1"
                     /translation="NWLQELGQRVITSEDSEQDKLNKTLAIFACGLMGFGAMLWLAIY
                     WAMGIKFSATVPLVYLGVSAGSLAYYLWTRNFELFRFIQVSLFLFVPFIMQWSIGSYV
                     SSSGVMLWALLAPVGVMIFQGPRESMPWFFAYIVMTGISGFFDYYLGEGTEQGVTMQS
                     IAVFFTLNFAAMSTIVYLLISYFIHQRDRLQESLDEQNKLLKQEQEKSERLLLNILPG
                     PIAERLKAQQTTIADGFADVTVMFADIIDFTRLSEEMPPRSMVTLLNEVFSQFDLLAE
                     KHGLEKIKTIGDAYMVAGGLDVHHLREEHRRAARDYSQAICELALDMRDYMSTLSGTR
                     RSPLQIHVGIGTGPVVAGVIGMKKFIYDLWGDTVNIASRVTSEAGPGAILVDSATYRR
                     VRREFEFEGPHVLNVKGKGEVTAYRLIGRTREIASSDAARVGA"
BASE COUNT          577 a          975 c          970 g          605 t
ORIGIN      
        1 atgtcgaaat agaggtagaa ctcccacgga tgcgggcgca gccgcacggg atccacctcc
       61 ttcttccact tgtaatcgag ccaggtctgg atcaaatcgt cggtgaatac gctgccctgc
      121 ttgaggaacc cgtggtcacc ctccagcgct cgcagcgatt cctccagcga gccgggcacc
      181 tgggcgatgc ccttggcttc ttcaggcggc agatcgtaga tgttcttgtc gagcggcgcc
      241 cccgggtcga tcttgttctg gacgccgtcg agccccgcca tcagcatcgc cgcgaatgca
      301 aggtaggcgt tggccgaagg atccgggcaa cggaactcta cgcgcttggc agcgggcgaa
      361 tccgaataca tcgggatgcg cgccgccgcg gaacggttac gctgggacat cgccaggttt
      421 acgggcgctt cgtagccggg caccagccgc ttgtaggaat tcgtcgtagg cgcgcagaac
      481 gccatcaacg ctttcgcgtg cttgagcagg ccgccgatat accaacggca cgcctgcgag
      541 gtcagcgcat agcccttggc gtcgaagaac aggttcttgt cgcccttcca caggctttga
      601 tggcaatgca tgccgctcgc attgtcggcg aaaatcggtt tgggcatgaa agtcgcgacc
      661 ttgccgtgct tgcgcgcgac gttcttgcag aagtacttgt acatcatcac gttgtcggcc
      721 atgcgcgtca gcggagcgaa gcgcatgtcg atctcgttct ggccggctgt ggaaacttcg
      781 tggtgatgca cctcgacctg tatgccggca tccatcagcg ccagcatgat ctccgaacga
      841 atgtcctgaa gggtgtcgtg aggcggaacc gggaaatagc cttccttgta gcgcggcttg
      901 tagccgaggt tgccgccgcc gtaggcgcct tcatcgcggc ctgagttcca ctcgccctcg
      961 gccgactcga cgtagtagta accgcaatgc tggttctgct cgaaccgtat cgagtcgaag
     1021 atgaaaaact cgagttcggg gccgaaatag caggtatccg cgatgccggt ctgcttgaga
     1081 tacgcctccg ccttcttcgc gatgtatcgt gggtcgcgtg aatagggttg cctgagcacc
     1141 gggtcgacga cgtcgcagat catcaccagc gtcggtatct cgcacatcgg atcgacaaag
     1201 gcggtgccgg ggtccggctt gagcagcatg tcggattcgt gtatctcctg gaagccgcgg
     1261 atcgaggagc cgtcgaagcc gatgccgtca tcgaacaagt ccttcttcag ctcgggcagg
     1321 ccgatcgaga agtgctgcca caggcccgga agatcgatga acctgagatc gatgatccgg
     1381 atgtcgttct tcctgacgaa ctccatcact tcctcgccgc tcgccggccg ctgcacgcga
     1441 taagagccgg gttccacttg gactaatgag cgctgctgct gggatgacat ggatgttctc
     1501 cttcctggtg ggtttgtcgt cgccggatgg cgatctcttc agtggcgccg tctttgccgc
     1561 ggctcggagt cttgcctaag ataactcgcg gcggcggaaa atcaagagga gccggcgctg
     1621 cgccggcagc cgacgccatt ccggtccagg ctcgcagccc gggcgcaggg cttgaaaggc
     1681 agtgcggctg gccgtattcc atctggcgca atcggtcatg atcctgcggg atgcgctcaa
     1741 agcacgacac cgcgacaccg aaggggcagc ccgtcacccg ggccgggccc ggcttgtaag
     1801 tcgtaataac tccgggggtt acgccccgac gcgggcggcg tcggatgagg caatttcccg
     1861 cgtgcgcccg atcagccggt acgccgttac ttcacccttg cccttgacgt tgaggacatg
     1921 cgggccctcg aactcgaact cgcggcgcac gcggcggtag gtggcggaat ccaccagtat
     1981 cgcgcccggg ccggcctcgg aggtgacccg gctcgcgatg tttaccgtgt cgccccacag
     2041 gtcgtagatg aacttcttca ttccgatcac gcccgccacc accggaccgg tgccgatgcc
     2101 aacgtggatc tgcagggggg acctcctcgt gcccgacagc gtgctcatgt aatcgcgcat
     2161 atcgagcgcc agctcgcaga tcgcctggct gtagtcgcgg gccgcccggc gatgctcctc
     2221 gcgcaggtgg tgcacgtcga gcccgcccgc caccatgtag gcgtcgccga tggtcttgat
     2281 cttctccagc ccgtgcttct cggcaagcag atcgaattgc gagaacacct cgttcagcag
     2341 cgtcaccatg gacctcggtg gcatctcctc cgacagccgc gtgaagtcga tgatgtcggc
     2401 gaacatcacg gtgacgtcgg cgaagccgtc ggcgatggtc gtctgctgcg ccttgaggcg
     2461 ctcggcgatg ggccccggca ggatgttgag cagcagccgt tccgacttct cctgctcctg
     2521 cttgagcagc ttgttttgct cgtccaggct ttcctgcagc ctgtcgcgct ggtggatgaa
     2581 gtagctgatc agcagataga cgatggtgga catcgcggcg aagttgagcg tgaagaacac
     2641 cgcgatcgac tgcatggtga cgccctgctc cgtgccctcg ccgaggtagt agtcgaagaa
     2701 accggaaatc cccgtcatca cgatgtaggc gaaaaaccat ggcatcgact cgcgcgggcc
     2761 ttggaagatc atcactccca ccggcgcgag caacgcccac agcatcacgc ccgaggagct
     2821 cacgtaactg ccgatgctcc actgcatgat gaacggcacg aacaggaaca ggctcacctg
     2881 gatgaagcgg aacagctcga agttgcgcgt ccacaggtag tacgccagcg agcccgccga
     2941 taccccgaga tagacgagcg gaaccgtggc ggaaaacttg atgcccatcg cccagtagat
     3001 ggcgagccac agcatcgcgc cgaagcccat cagcccgcag gcgaagatcg ccagcgtctt
     3061 gttgagcttg tcctgctcgc tgtcctcgct ggtgatgacg cgctggccca gttcctgcag
     3121 ccagttt
//
LOCUS       DBNGWZ010000156         1862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_769714, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000156 DBNGWZ010000000
VERSION     DBNGWZ010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_769714"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="AC16CX_04695"
     CDS             complement(<1..631)
                     /locus_tag="AC16CX_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011847.1"
                     /translation="MSQSRSFPLQGIKVIELGTLIAGPFCARILAEFGAEVIKIEPPD
                     GGDPLRKWRKLHQGTSLWWYAQARNKKSVTLNLKHPEAQEIVRKLVRDADVVVENFRP
                     GAMEKWGLGWEQLSRINPGLVMVRLSGFGQTGPYRDRPGFGAIGESMGGMRYVTGYPD
                     RPPVRVGVSIGDSIAALHGVIGALMALHHRNLNGGRGQYVDVALYEAVFN"
     gene            867..1115
                     /locus_tag="AC16CX_04700"
     CDS             867..1115
                     /locus_tag="AC16CX_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3011848.1"
                     /translation="MNWDRVEGNWRQLRGKFKEQWGVLTEGQLDIIAGKRERLIGVLQ
                     ESYGISKEEAERRVNEWEKNEQRIAAFEQVLRRTGEMV"
     gene            1427..1648
                     /locus_tag="AC16CX_04705"
     CDS             1427..1648
                     /locus_tag="AC16CX_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HIR3011849.1"
                     /translation="MNTTRLTSYAAAGVLCLALGACTGNPTNAQYGIAAGVLIGSVIG
                     SGLTAGSITGTAAGAVIGGVVGYQIGKQL"
BASE COUNT          365 a          553 c          590 g          354 t
ORIGIN      
        1 tgttgaacac cgcctcgtag agcgcgacat ccacgtattg cccgcgcccc ccgttcaggt
       61 tgcgatggtg cagcgccatc agcgcgccga tcacgccgtg cagcgcggcg atggagtcgc
      121 cgatcgagac cccgacccgc accggcggcc ggtcggggta gccggtgaca taacgcatcc
      181 cgcccatgga ctccccgatt gcgccaaagc ccggccggtc gcgatagggc ccggtctggc
      241 cgaatccgga gagccggacc atgacaagcc ccgggttgat cctcgaaagc tgttcccagc
      301 cgagtcccca cttctccatc gcgcccggac ggaagttctc gaccacgacg tcggcgtcac
      361 ggaccagctt gcgcacgatc tcctgcgcct cggggtgctt gaggttcagc gtcaccgact
      421 tcttgttgcg cgcctgcgcg taccaccaga gcgaggtccc ttgatggagc ttgcgccatt
      481 tcctcagggg atcgccgccg tcgggcggct cgatcttgat cacctccgcg ccgaattccg
      541 ccaggatgcg cgcacagaac ggtccggcga tcagggtgcc gagctcgatt accttgatgc
      601 cttgcagcgg aaaagaacgc gattggctca tggcgggagg aaattctacc gatttgtccc
      661 agttgcttgg agctatacgc gcgtttccgg gcccgcgtcg cccgaggacg acgatttcgt
      721 tgcgatctca tcccggcgtt tccccataac aacttgtttc ttatcgatat gcgcgagctg
      781 gcacggcccg tgctgaccgt cccggaaagg ttgcgagcgc gcgcggcacc ggaaaccttc
      841 aggtgacacc acgaaaggag catggaatga actgggatcg tgtcgaaggg aactggagac
      901 agctgagggg caagttcaag gagcaatggg gcgtgctcac tgagggccag ctcgacatca
      961 tcgcgggcaa gcgggagcgg ctgatcggcg tgctccagga aagttacggt atctcgaagg
     1021 aagaggccga gcggcgggtg aacgaatggg agaaaaacga acagcgcatt gccgcttttg
     1081 agcaggtgct gcgccgcacc ggcgagatgg tgtagccgga ttcgggtggc agcgaccgca
     1141 gtgtcgggaa tcggcgacag taaaaatcct ccatggctca gccggatgcc atgagccgta
     1201 actattgccg tctctgcgca gcgacatttt actggggatc accgtccgcg attttcacca
     1261 actgattgat cttgaaggaa aattcatttt ggcacggctc gtgcgtggta tgaagtgaca
     1321 cggaaccggc tcctccgtag cagcagcagc agtagttcca ttccctcccc tgttttcccc
     1381 ggcgtaagcc ggggtttttt ttcaactcga catgcggagc gtgcacatga atacaaccag
     1441 gcttacatcc tatgcggcgg caggcgttct ctgcctcgcg ctcggcgcgt gcacaggaaa
     1501 tccgaccaat gcgcagtacg gcattgcggc gggggtgctg atcggcagcg tgatcggatc
     1561 cgggttgacc gcgggcagca tcaccggtac cgccgccggc gccgtgatcg gcggcgtggt
     1621 cggttaccag atcggcaaac agctctagtt gcgcggagcg ctgctccgcg gtgatgggtg
     1681 atgggtgata ggagtccaaa accggcgatg ccggtcattc ctatcacata tcattggcca
     1741 aggctcagcg ttcgccgaac acctgaggct cagcgttcgc cgaacacttg cgatagggta
     1801 aaggattttg gggtgagtta gtcatggcga aaaaagctcc attgaacgca agcgtcgcga
     1861 ga
//
LOCUS       DBNGWZ010000157         2251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2658636, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000157 DBNGWZ010000000
VERSION     DBNGWZ010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2658636"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="AC16CX_04710"
     CDS             <1..866
                     /locus_tag="AC16CX_04710"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011850.1"
                     /translation="AFAPGGSVDVVARLLAQKLGEAWKQQVVVDNRPGAGGNVSAEIV
                     TRAANDGHTIYMCSASFVVNPSLYAKILYDPVKDFAPVSLVSLVQNVLVAYPGLPVKS
                     VKELLALARAKPGQINYASTGSGTSGHLVMELFKSTAGISLVHVPYKVIGQAQTDLMS
                     GQVSLWFPTAPGALPHIQAGRMRVLAVAGAKRSPALPEVPTVSEAGVPGFEASTWYAM
                     LAPAGTSHEIVSKLNGAVIGVLKQPDVRERLSGMAIEVVGSSAEQLARHIRTEIPKWA
                     RVIKLSGARVD"
     gene            999..>2251
                     /locus_tag="AC16CX_04715"
     CDS             999..>2251
                     /locus_tag="AC16CX_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3011851.1"
                     /translation="MIRIHRRSFFIMILLLAASLLYAPIAGAAEPPKLDPANTAWMLT
                     SSALVLFMTLPGLALFYAGLVGARNVLSVLMQCFAITCVVTLAWVIFVYSIAFGDGGA
                     SNAWYGGFAKSFMSGVEFKTLKGSIPESVFAMFQLTFAIITPALVLGAYAERVKFSGM
                     LLFSLLWLIFVYAPITHWVWGDGWLQKMGIMDFAGGTVVHLNAGMAALVCALVLGRRR
                     GFPETPMPPHNLTMTVTGACMLWVGWFGFNAGSALAADGSAGMAMLVTQIGAATASLA
                     WMFCEWVRFGKPSVLGIVTGMVAGLGTITPASGFVGPIGALVIGAAAGVACFFATNFM
                     KRTMHVDDSLDVHPVHGVGGALGTTLTGVFAAAAFGGVGYPEGMSMGGQVLVQLTGVL
                     AVAVWSGAVTWILLKATDVVVGLRVT"
BASE COUNT          337 a          702 c          775 g          437 t
ORIGIN      
        1 tcgcattcgc gcccggtggc agcgtcgatg tcgtcgcgcg gttgctggcg cagaagctcg
       61 gcgaggcctg gaagcagcag gtcgtggtgg acaaccggcc cggcgcgggc ggcaatgtca
      121 gcgccgagat tgttacgcgc gccgccaacg acggccacac gatttacatg tgcagcgcct
      181 cgttcgtggt caatcccagc ctttacgcca agatactcta cgatccggtc aaggatttcg
      241 ctccggtgtc cctggtgtcg ctcgttcaga acgtgctggt ggcatatccg gggcttcccg
      301 tgaaatcggt gaaggagctg ctggcgctgg ccagagcaaa accggggcaa atcaattacg
      361 cgtccacggg cagcggcacc tcgggccacc ttgtcatgga gttgttcaag tccacggccg
      421 ggatcagtct cgtgcacgtg ccgtacaagg tgatcggcca ggcccagacc gatctcatga
      481 gcgggcaggt ttcgctctgg ttcccgaccg cgccgggcgc gctgccgcat atccaggcgg
      541 gcagaatgag ggtactggcg gtcgcgggcg cgaaacgctc gcccgcgctg ccggaggttc
      601 cgacggtgtc cgaggccggc gtccccggct tcgaagcgtc cacctggtac gcaatgcttg
      661 cgccggcagg cacctcccac gagatcgtca gcaagctcaa cggcgcggtg atcggggtcc
      721 tcaagcagcc ggatgtccgt gagcgcttgt ccggcatggc gatcgaggtg gtgggcagca
      781 gcgccgagca gcttgcccgg cacatccgca ccgagatccc gaagtgggcc cgggtcatca
      841 agctctccgg cgcccgcgtc gactgaaatc cgcgaaaaat cgcggcgcat caagccgctc
      901 gcgcgcgtcg ctcgccaatg cttgaccgaa gcgcggtgcg cagtccatca ttggcatgcc
      961 gcctgtagca gcccgcggcg cgcgtaaggg aggagtcaat gataagaatt caccgccgtt
     1021 ctttttttat catgatcctg ttgctggccg cgtcgctgct gtacgcgccg atcgccggtg
     1081 ccgccgagcc gccgaagttg gaccccgcaa ataccgcgtg gatgctgact tcatcggcgc
     1141 tggttctctt catgaccctg ccgggccttg cgctgttcta cgcggggctg gtgggcgcga
     1201 ggaacgtcct ttcggtcctc atgcagtgct ttgcaatcac ctgcgtggtc acgctcgcat
     1261 gggtgatttt cgtttacagc atcgcgttcg gtgacggtgg tgcgagcaac gcgtggtacg
     1321 gcgggttcgc gaagtccttc atgtccggcg tcgaattcaa gacgctcaag ggcagcattc
     1381 cggagagcgt gttcgcgatg ttccagttga cgttcgcgat cattacgccg gcgctcgtcc
     1441 tcggcgccta cgccgagcgc gtgaagttct ccggcatgct gctcttcagc ctgctctggc
     1501 tgatcttcgt ctacgcgccg atcacgcact gggtgtgggg cgacggctgg ctgcagaaga
     1561 tggggatcat ggactttgcg ggcggcaccg tcgtgcacct gaacgcgggc atggcggcgc
     1621 tggtctgcgc gctggtgctg gggcggcgca ggggatttcc cgagacaccc atgccgccgc
     1681 acaacctcac gatgacggta actggcgcgt gcatgttgtg ggtaggctgg ttcggcttca
     1741 acgccggcag cgcgctggcg gccgacggct ccgcaggcat ggcgatgctg gtgacgcaaa
     1801 tcggcgcggc gacagcatcg ctcgcgtgga tgttctgcga gtgggtgcgc ttcggcaagc
     1861 cctcggtgct cggcatcgtc accggcatgg tcgcggggct aggcacgatc acgccggcct
     1921 ccggtttcgt cgggccgatc ggcgcgctcg tgatcggcgc cgccgcgggt gtcgcgtgct
     1981 ttttcgccac caatttcatg aagcgcacga tgcacgtgga cgattcgctc gacgtgcacc
     2041 cggtgcacgg ggtcggcggc gcattgggta cgacgctcac cggcgttttc gcggcggccg
     2101 ctttcggcgg cgtaggctat cccgaaggca tgtcgatggg cgggcaggtg ctggtgcagc
     2161 tcaccggtgt gctcgcggtg gctgtgtggt ccggcgcagt cacctggatc ctgctcaagg
     2221 ccaccgatgt cgtcgtgggc ctgcgcgtga c
//
LOCUS       DBNGWZ010000158         2789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2282275, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000158 DBNGWZ010000000
VERSION     DBNGWZ010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2282275"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="AC16CX_04720"
     CDS             complement(<1..748)
                     /locus_tag="AC16CX_04720"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HIR3011852.1"
                     /translation="MRGYADLHEHLEELKQRGLLLTIDRPINKDAELHPLVRWQYVGG
                     VEEHERRAFLFTNVVDGRGRKYKFPVVVGAYAGSRAIYCIGMRASAEEVQPKWDHAIT
                     NPIPPRIVAAAPCQEVVIEGDALRGEGNGLDSLPIPISTPGFDSAPTLTATNVVTRDP
                     ETGIQNHGTYRAGLKAPDRCVIRMATRTGGAGGYRHYLKHQKRGDKTMPCAIVLGAPL
                     CVAFVAPMKLPLDLDEIGVAGAVAGEPINVV"
     gene            complement(876..1877)
                     /locus_tag="AC16CX_04725"
     CDS             complement(876..1877)
                     /locus_tag="AC16CX_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011853.1"
                     /translation="MTSNRKSTWLPGDVACALAGLVVAWGVGNAAAQDIAYPTRTVQI
                     VVPYTPGTGADILARLFGPKLSERWKVGVVTDNRPGATGNIGAEIVSKAAPDGHTILF
                     TATSFGTTPALYRKLPFDPVKSFAPVALIATSGLALVVHPQLPVKSVKEFIQLAKRRP
                     GQMLYSSPGNGGPQHLAMELFKLETGIDIVHVPYKGLAGAITDVIAGHVQAMISATQT
                     AHSAVQSGRLRVLAVMSPERSPAYPEAPTVKELGLAQVEVETWYGSFAPGATPAPVVA
                     KINAELNALLQLPDVRDSLAKQGMTPRGGTPERFGSMVRAELARWDRVVKAAKIKAD"
     gene            1997..>2789
                     /locus_tag="AC16CX_04730"
     CDS             1997..>2789
                     /locus_tag="AC16CX_04730"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3011854.1"
                     /translation="MNDHAWVINDPEAMRFQVHRSTLVDPQILELERRRVFDVCWIYV
                     GHESEVRAPGDFKTRIVCDRPVIFCRDGKNRVRVFLNTCRHRGSIVCREPEGNAKGYT
                     CFYHGWSYNRDGELDGVPGLDAFPPSFRKSDFGMKEPPHVAIYRGFVFLNFDPDAMPL
                     EDYLAGAKEYIDIVVDQSPSGQMEVIQGVQDYDIRANWKLLAENSFDDYHLLSVHGSW
                     LNYLKSSGVEMKRPEKGHLLPAHGVGKELGNGHGCTDNVNFRGRPV"
BASE COUNT          463 a          910 c          890 g          526 t
ORIGIN      
        1 gcaccacgtt gatcggctcg ccggcgacgg cgcctgcaac accgatttcg tcgaggtcga
       61 gcggcagctt catcggcgca acgaacgcga cgcagagcgg cgcgcccagc acgatcgcgc
      121 acggcatggt cttgtcgccg cgcttctggt gcttgagata gtgacggtaa ccgcccgccc
      181 ctccggtgcg ggtcgccatg cggatcacgc agcggtcggg cgccttgaga cccgcgcggt
      241 aggtgccgtg gttctgtatg ccggtttcag ggtccctggt gacgacgttg gtcgcggtga
      301 gcgtcggcgc gctgtcgaag ccgggcgtcg agatcgggat cggcaggctg tcgagcccat
      361 tgccctcccc tctcagcgcg tcgccttcga tcacgacttc ctggcagggc gcggctgcga
      421 cgatgcgcgg cggtatcggg ttggtgatcg cgtggtccca cttgggctgc acctcttcgg
      481 ctgacgcgcg catcccgatg caataaattg cgcggcttcc cgcatacgcg ccgacgacga
      541 ccgggaactt gtatttccgg ccgcgcccgt ccacgacgtt ggtgaacagg aaagccctgc
      601 gctcgtgctc ctcgacgccg ccgacgtact gccagcgcac caggggatgc agctccgcgt
      661 ccttgttgat cggccggtcg atggtgagga gcagcccgcg ttgtttcagc tcttcgaggt
      721 gctcgtggag atcggcgtag ccgcgcactg ccttaccccc gtcacgactg acgactgacg
      781 acagacgggc ttttgcttgt ttttccgcca tctactgtcc ctttgctgca atcaattgtt
      841 gttcatcagc catcagtcgt cagccgtcac cggttctaat cagccttgat cttcgccgcc
      901 ttgaccacgc gatcccagcg ggcaagttcc gcgcggacca tgctgccaaa acgctccggg
      961 gtgccgcctc gcggcgtcat gccctgcttg gccagcgaat cgcgcacatc gggcagctgg
     1021 agcagcgcgt tcagctcggc gttgatcttc gccacgaccg gcgccggcgt agcgcccggc
     1081 gcaaatgagc cgtaccacgt ctcgacttcg acttgcgcga ggcccagctc cttaactgtg
     1141 ggcgcctctg ggtacgccgg cgagcgttcc gggctcatta ccgcgagcac gcgcagcctg
     1201 ccggattgca cggccgagtg cgccgtctgc gttgccgaga tcatcgcctg cacgtggccc
     1261 gctatgacgt cggtgatggc gccggccagc cccttgtacg gcacgtgcac gatatcgatg
     1321 ccggtttcga gcttgaacag ctccatcgcg aggtgctgcg gcccgccgtt gcccggtgac
     1381 gagtacagca tctgtcccgg gcggcgtttc gcaagctgga tgaattcctt gaccgatttc
     1441 accggcagct ggggatggac gacgagcgcg agcccgctgg tcgcgatcag cgccaccggc
     1501 gcgaaactct tgaccggatc gaatggcagc ttcctgtaaa gcgccggcgt cgtcccgaac
     1561 gaggttgccg tgaataatat cgtgtgcccg tccggcgccg ccttcgaaac gatttcggcg
     1621 ccgatgttgc cggtcgcacc cggccggttg tcggtgacga cgccgacttt ccagcgctcg
     1681 gaaagcttcg gcccgaaaag gcgcgcgagg atgtccgcgc cggttcccgg cgtgtacggc
     1741 acgacgatct ggacagtgcg ggtcgggtag gcgatgtcct gcgcggccgc gttcccgacg
     1801 ccccaagcca cgacgagtcc cgcgagggca cacgcaacat ctcccgggag ccacgtactt
     1861 ttccggttgc tcgtcatctg cgatctcctc ggacaatgat tattctagga cgagcattat
     1921 tataggattt tggcagctga cgaagcgaca aatacctgat tgacatcgga cggcagtctt
     1981 gcgactctat ggacgtatga acgatcacgc ctgggttatc aacgacccgg aagcgatgcg
     2041 cttccaggtc caccgctcga cgctggtgga cccgcagatc ctcgagctcg aacggcgcag
     2101 ggtcttcgat gtctgctgga tctacgtggg ccacgagtcg gaggtccgcg cgcccggcga
     2161 cttcaagacc cgcatcgtct gtgaccggcc cgtcatcttc tgccgcgacg gcaagaaccg
     2221 ggtccgcgtc tttctcaata cgtgccggca ccgcgggagc atcgtctgcc gcgagcccga
     2281 gggcaacgcg aagggctaca cctgttttta ccacggctgg agctacaacc gggacggcga
     2341 gcttgacggc gttcccggcc tggacgcatt tccaccgagc ttcaggaaaa gcgatttcgg
     2401 catgaaggag ccgccgcacg tggcgatcta ccgcggcttc gtcttcctca atttcgaccc
     2461 ggacgcgatg ccactggagg attacctcgc cggcgccaag gaatatatcg acatcgtcgt
     2521 ggaccagtcg ccctccgggc agatggaggt gatccagggc gtgcaggact atgacatccg
     2581 cgccaactgg aagctgctgg cggagaacag cttcgacgac taccacctgc tgagcgtcca
     2641 cgggtcgtgg ctcaattacc tgaagagctc gggggtcgag atgaagcgcc ccgagaaggg
     2701 tcatctcctc ccggcgcacg gcgtgggcaa ggaactcggc aacggccacg gctgcaccga
     2761 caacgtgaat ttccgcgggc ggccggtgg
//
LOCUS       DBNGWZ010000159         1557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2534478, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000159 DBNGWZ010000000
VERSION     DBNGWZ010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2534478"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="AC16CX_04735"
     CDS             complement(<1..249)
                     /locus_tag="AC16CX_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3011855.1"
                     /translation="MSLTNEDEALFHAYCRELRRNCLVDYDDIIFLTRLLLERDTGVL
                     AGYQTRWDHLLVDEFQDLDPCQYEILRALAERHRSVFAV"
     gene            complement(434..1491)
                     /locus_tag="AC16CX_04740"
                     /pseudo
     CDS             complement(434..1491)
                     /locus_tag="AC16CX_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611458.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          235 a          570 c          461 g          291 t
ORIGIN      
        1 aaccgcaaac acgctgcggt ggcgctcagc gagcgctcgc agtatctcgt actgacacgg
       61 atcgaggtcc tgaaactcgt cgacgaggag gtgatcccag cgcgtttggt agccggccaa
      121 taccccagta tcgcgctcga gcagaaggcg agtgaggaag ataatgtcgt cgtagtccac
      181 gaggcagttt cggcgcaact ctcggcagta cgcgtggaac agggcttcat cttcattcgt
      241 gagggacatg ccgccgagcc tgggctgtcc ccattcttcg ggccaccggc tatgacaggg
      301 tctccggcgt gatggggtga acttctgctt cgggtctctc gagatcggct tccagatttg
      361 ccccaggaac gaaagttgac acctcctccg actccggagt catggccgcg cgggcctctg
      421 cgccgacttc cgccggcgtc cggtagcgca gactgctgtg cgggcgctgc gtgttgtact
      481 cccaccgcca ctgctcgatc acgatcttcg cctcgcgcag gttcgcgaac cactcgcgat
      541 ccaggcactc ggcgcgcaac gtcccgttca agctctccga gctcccgttc tgccacggct
      601 tgcccggctc gatcgtggcg acctcgctgt ggttcatccg cgcccagatc ctcagcgccc
      661 gggcgatgaa ctcggggccg ttgtcgctgc gcagcacggc cggggtcccg tactgtgcga
      721 acagctgctc gagcaccgcg atcacccgca gggcgtcgat cgacgtcgcc acctcgatcg
      781 ccaagcactc ccgcgtccac tcgtccttca ccgtcagcgc cttgaactgc tgcccgttgc
      841 cgcaggtgtc gtggacgaag tcgtacgccc agacctgatt cgctcccgtc ggggtcagca
      901 cccgcggctg acccgtgcgc agcttgcgcc gcttcttccg cgacgcgcac agccccaaca
      961 accgccacac ccgccgcacc cgcttgcggt tcaccgaccg cttccgctgc tgcgtcagca
     1021 ccgcccacgc gaaccgatgc ccgtagcgcg ggtgggccgc cgcgatctgc tgcagctccg
     1081 cggccagctc agcgtcccgc tccggcatcc gactcgtgta ctccagggcc gaacggctca
     1141 cctgcagcag cgcacaggct cggcggaccg acgctccccg actcacggcg tagtccacct
     1201 gttcacggcg agccggtgcg ctcaccattt ttttgcgttg atctccttca ggatatcgat
     1261 gtccagcgcc tgctgggcca ccacccgctt gagccgcgtg ttctcgtgct gcagctgccg
     1321 cagctccgtg gcctggctcg gcgtcagccc gccgaaccgc ttccgccacc ggaacagcgt
     1381 ggtctccgtg atcccgtgct tcttcgcgac ctgcgcgacc gttacgcctt cgcgctccga
     1441 ctcgcgcacg atggcgacga tctgttcctc cgtgaaccgg ctcttgcgca tgcaggatcc
     1501 tcctgggccg gagagatcct gccataatcg gtggcctcaa gaacgggggt cagtcca
//
LOCUS       DBNGWZ010000160         4006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_897467, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000160 DBNGWZ010000000
VERSION     DBNGWZ010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_897467"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16CX_04745"
     CDS             complement(<1..242)
                     /locus_tag="AC16CX_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydrolyase"
                     /protein_id="HIR3011856.1"
                     /translation="MAHFDFTTPVTEEQVRKLRVNDTVTLDGTLYGIRDATQIHMFDR
                     GRKTRFDLRGHALLHTAPNVRKVAKSRQHPTGYAPI"
     gene            complement(357..1232)
                     /locus_tag="AC16CX_04750"
     CDS             complement(357..1232)
                     /locus_tag="AC16CX_04750"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR3011857.1"
                     /translation="MKVDLKQVEDAAKELYIRALKVLPPDVKEGFVRLDRGETNATGK
                     AILGTMIRNIQVAEQNDNLLCQDTGIPIYNVWIGRNVEVDGAALKEAIRRGCARSTRE
                     YSFRSSIVHPTTRVNEQTSCGPNVPVIHFDFTGRADTLEIEMIPKGSGSENNSWLKMA
                     VPADGVDAIKTFVVDCVLDAGGKTCPPTIVGVGVGGTADLCVHLSKVAATRPLGSRCE
                     DPEGAKLEQELTRAVNRLGVGPQGLGGDSTAFAVHVEIAATHITMNPVAVNMQCHSAR
                     RARATFTPKGITYGF"
     gene            complement(1279..2241)
                     /locus_tag="AC16CX_04755"
     CDS             complement(1279..2241)
                     /locus_tag="AC16CX_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011858.1"
                     /translation="MRFTKSIALTAAAFFAFAAHAQTYPAKPVRIIVPFAPGGATDIV
                     TRILAQKLTEAWGQQILVDNRAGAGGNVGGELAARASADGYTLFMTSGSIVTANQYMY
                     KKLNWNPEKDLVAITNVASGPQIVAVHPSFPAKSIKEMIAIAKARPDTITFGSAGVGT
                     QTHLAAENFLHTARINAVHVPYKGEGPALVDLIAGQIIFLTPNLSAAIGYVQQGRLRA
                     LGVTSRKRAPQLKDVPAVAETLPGFENLGWFGLMAPTGTPAAVIEKVHRDTARALEGA
                     DLRKRFDDIGMAPVGNAPADFAKAIREEAGRWAKVIRERKLEVN"
     gene            complement(2323..3696)
                     /locus_tag="AC16CX_04760"
     CDS             complement(2323..3696)
                     /locus_tag="AC16CX_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3011859.1"
                     /translation="MRRPNFLVILIDDLRYDEFGAGGHPYMKTPAIDRIASEGAIFGR
                     AFHTTPICSPNRASILTGQYASRHGIIDNVARDAASHRLPNYHLELQRLGYETAHIGK
                     WHMGNNGQPRPGYDFWVSFDGHGRLNDPRLNEHGHYVQRTGYITDILNALAVDFVSRK
                     RDKPWSLFLAHKAVHPDAEQAADGTLALDGYKPAARHEKLYQGCVFPKKPNMLAPAEV
                     VKQKPAWAEAFELKRNERSQALLRAIHAGQQEEIRLRAAMMASVDEGVGMLFDALAST
                     RQLDDTLILFLGDNGYFFGEHGLGPERRFAYEEGIRSPFVLRYPRGVRAGSRVRDLVI
                     LQDIAPTLIELARGRPAPHVQGRSLLPLFAGRRAGWRRSFLVEYWAENAWPWLIGMTY
                     KAVRTDRHKYIRWVNRGRNGELDELYDLDRDPHEIRNVIHRPGYRTVCDRLRRELRRL
                     VVEAGGL"
     gene            complement(3807..>4006)
                     /locus_tag="AC16CX_04765"
     CDS             complement(3807..>4006)
                     /locus_tag="AC16CX_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011860.1"
                     /translation="GLPADLVAKLNADIGRALVSAEVKQRLTADAAEPAGGSPGDLAV
                     YLKADYERWGKVVRASGIRPE"
BASE COUNT          617 a         1353 c         1301 g          735 t
ORIGIN      
        1 caaatcggcg cgtagcctgt ggggtgctgc ctggacttcg caaccttcct gacgttgggg
       61 gcggtgtgca gcagcgcgtg gccgcgcaga tcgaagcggg tcttgcgccc gcggtcgaac
      121 atgtggatct gcgtcgcgtc gcggatgccg tagagcgtgc cgtcgagcgt caccgtgtcg
      181 ttgacgcgga gctttcgcac ctgctcctcg gtgaccggcg tggtgaagtc aaagtgcgcc
      241 ataacaaatt ccgggagatc gggaggtcgg gagatcggga gatcgggaga tggaaacacc
      301 ccgaatgctg gttcgatctt cgctctcacg ccctcccgct ctcacgctct cccgtcttaa
      361 aagccgtagg tgatgccctt cggcgtgaag gtcgcgcgcg cgcggcgggc ggaatggcac
      421 tgcatgttga ccgccaccgg gttcatcgtg atgtgggtcg cggcgatctc cacgtgcacc
      481 gcgaacgcgg tcgaatcgcc ccccagcccc tgcggcccca cgccgagccg gttgaccgcg
      541 cgggtaagct cctgctcgag cttcgcgccc tccgggtcct cgcagcggct gccgagcggg
      601 cgcgtcgcgg ccactttcga gaggtgcacg cacaggtccg ccgtgccgcc cacgccaacg
      661 ccgacgatcg ttggcgggca ggtcttgccg cccgcatcga gcacgcaatc cacgacgaag
      721 gtcttgatcg cgtccacgcc gtcggccggt accgccatct tgagccacga gttgttctcc
      781 gagcccgagc ccttgggaat catctcgatc tccagcgtat cggcgcggcc ggtaaagtcg
      841 aagtggatga ccggaacatt ggggccgcac gaagtctgct cgttcacgcg ggtggtgggg
      901 tgcacgatcg aggacctgaa ggaatattcg cgcgtcgagc gcgcgcaacc gcgccggatc
      961 gcttccttga gcgcggcccc gtccacctcg acgttgcgcc cgatccagac gttgtagatc
     1021 gggatgccgg tgtcctggca caacaggttg tcgttctgct cggcgacctg gatgttgcgg
     1081 atcatggtgc cgaggatcgc cttgccggtc gcgttcgtct cgccccggtc gaggcgcacg
     1141 aatccctcct tgacgtcggg cggcagcacc ttgagggcgc ggatgtaaag ctccttcgcc
     1201 gcgtcctcga cctgcttcag atcaaccttc acacgtcact cgacactcga cactctaatt
     1261 gaccttcccg gcgcgcgatc agttcacttc cagcttgcgc tcgcggatca ccttcgccca
     1321 gcgtccggct tcctcccgga tcgcttttgc gaaatccgca ggcgcgttgc cgaccggcgc
     1381 catgccgata tcgtcgaagc gcttgcgcaa gtccgcgcct tcgagcgccc tcgcggtgtc
     1441 gcggtggacc ttctcgatga ccgccgcggg cgtgccggtg ggcgccatca ggccgaacca
     1501 tccgaggttc tcgaagcccg gcagcgtctc ggcgaccgct ggcacgtcct tcagttgcgg
     1561 cgcgcgcttc ctgctggtga cgccgagcgc gcggagccgg ccctgctgca cgtagccgat
     1621 cgccgcggaa agattgggcg tcaggaatat gatctggccc gcgatgaggt ccaccagcgc
     1681 cgggccctct cccttgtacg ggacatgcac cgcgttgatc cgcgcggtgt ggaggaagtt
     1741 ctccgccgcc aggtgcgttt gcgtgccgac cccagcggag ccaaaggtga tcgtgtccgg
     1801 cctcgccttg gcgatggcaa tcatctcctt gatgcttttc gccgggaagc tcggatgcac
     1861 ggcgacgatc tgaggcccgc tcgccacgtt ggtgatcgcc accaggtcct tttccgggtt
     1921 ccagttgagc ttcttgtaca tgtactggtt cgcggtgacg atggagcccg aggtcatgaa
     1981 cagcgtgtag ccgtcggcgg acgctctcgc cgcgagctcg ccgccgacgt tgccgccggc
     2041 gccggcccgg ttgtccacca ggatctgctg gccccacgcc tccgtcagct tctgcgccag
     2101 gatgcgcgtc acgatgtcgg tggcaccgcc cggcgcgaac ggcacgatga tccgcaccgg
     2161 cttggcggga taggtctggg cgtgcgccgc gaatgcaaag aaagccgctg ccgtgagcgc
     2221 gatcgatttt gtgaagcgca tgattccctc ctgatcatgg tttttagact tgcagttcga
     2281 accgcctaac catactccac ggcccccacg ccagcaagac gattacagcc cgcctgcctc
     2341 cactacgagc cgccgcaact cgcggcgcag ccgatcacac accgtgcgat atcccggccg
     2401 atggatgacg ttgcggattt cgtgcgggtc ccggtcgaga tcgtagagct cgtcgagctc
     2461 gccgttccgg ccgcggttca cccagcgtat gtacttgtga cggtcggtgc gcacggcctt
     2521 gtaggtcatg ccgatgagcc acggccatgc attctccgcc cagtactcga cgagaaatga
     2581 tcttcgccac ccggcgcgcc ggccagcgaa caagggcagc agcgagcggc cctgaacgtg
     2641 cggtgcgggc ctgccgcggg cgagctcaat gagggtcggg gcgatgtcct gaagaatgac
     2701 gaggtcccgc acgcgcgagc cggccctgac cccgcgcgga tatcgcaaaa cgaacggcga
     2761 gcggatgccc tcctcgtacg cgaagcggcg ctcgggcccg aggccgtgct cgccgaagaa
     2821 gtagccgttg tcgccgagga agagtatcaa cgtgtcgtcg agctggcgtg tgctggcgag
     2881 cgcgtcgaac agcattccga cgccctcgtc caccgacgcc atcatcgccg cgcgcaggcg
     2941 aatctcctcc tgctgcccgg cgtggatcgc cctgagcagc gcctgtgagc gctcgttccg
     3001 cttcagctcg aaggcctccg cccacgccgg cttctgcttc accacttccg ccggtgcgag
     3061 catgttcggc ttcttcggga acacgcagcc ctggtacagc ttctcgtgcc gcgcggcggg
     3121 cttgtagccg tcgagcgcca gcgtgccgtc cgccgcctgc tcggcgtccg ggtgcaccgc
     3181 cttgtgcgcg aggaacagcg accacggctt gtcgcgcttc cggctcacga agtccaccgc
     3241 caacgcgttc aggatgtcgg tgatatagcc ggtgcgctgg acgtaatggc cgtgctcgtt
     3301 caaccgcggg tcattcagcc gtccgtgtcc gtcgaagctc acccagaagt cgtagccggg
     3361 ccgcggctgc ccgttgttgc ccatgtgcca cttgccgatg tgggcggtct cgtagcccag
     3421 ccgctgcagc tcgaggtgat aattgggcag gcggtggctc gcggcgtcac gcgccacgtt
     3481 gtcgatgatg ccgtgccggc tcgcgtactg gccggtgagg atcgaggccc gattgggcga
     3541 gcagatcggc gtcgtgtgaa aggcgcgccc gaaaatcgcg ccttcgctcg cgatgcggtc
     3601 gatggccggc gtcttcatgt acgggtggcc gcccgcgccg aactcgtcgt aacgcaggtc
     3661 atctatgaga atgacgagaa aattaggtcg tcgcattgaa tgacctccga gccacaagca
     3721 cttagcaacc agctctcagc gatcggcccg gactgcttgc gccttgcctc aagctgagcg
     3781 ccgagagctg atcgctgaat gccgcactac tcgggacgga tgccggaagc cctcaccacc
     3841 ttgccccagc gctcgtagtc ggccttgagg taaacggcga ggtcgcccgg cgagccgccc
     3901 gccggctccg ccgcgtccgc ggtgagacgc tgcttcacct cggcgctgac cagcgctctt
     3961 ccgatgtcgg cgttgagctt cgccacgaga tccgccggca ggccga
//
LOCUS       DBNGWZ010000161         3531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2408933, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000161 DBNGWZ010000000
VERSION     DBNGWZ010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2408933"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="AC16CX_04770"
     CDS             complement(<1..223)
                     /locus_tag="AC16CX_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011861.1"
                     /translation="MKTISRIVLAALLCGAAAGWAQAYPAKPIRLIVPFARGRRTGSR
                     RGGWCWGGDGAQPPGLWHCALRRVAIALVA"
     gene            complement(370..1605)
                     /locus_tag="AC16CX_04775"
     CDS             complement(370..1605)
                     /locus_tag="AC16CX_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3011862.1"
                     /translation="MSAQGRALVIGGSLGGLFAANWLRAIGWDVAVFERVEDDLASRG
                     AGIGTHEELLAVLRRLGLRVDESIGINVRKRICLDSSGKLTHRLELSQIQSSWARIYR
                     LLKDRFPADSYRFGMSLERVEQEADGVTAVFTDGSRESGDLLVGADGIRSTVREQLLP
                     GVQPRYAGYIAWRGVLDERDVPPAVHAEIFELYSMCLPEGEMMLSYPVPGRDNDTHPG
                     RRGYNFVWYRPTEEREALARMCTDATGRCHGVSIPPPLIRPEVVAHVKADARALLAPQ
                     IATIVERTAQPFFQAIFDLESPRIALGRVVLLGDAAFVARPHVGMGVTKAALDAQCLA
                     SAIREARGDLAAALERYDRERRLFGTRIGARARRLGAYIEAQVKPPAERTEAERQPPP
                     EFLMREIGSASVDIQALTA"
     gene            1778..2191
                     /locus_tag="AC16CX_04780"
     CDS             1778..2191
                     /locus_tag="AC16CX_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR3011863.1"
                     /translation="MEANLPQASVSPPSDGMVTLTHVLYGLHGFSALMGALGPVLVVT
                     AFLTGWPSIIAIIINYVKRGDARGTWLDSHFGWQLRTFWYAVLWVVIAGLVFLTVIGI
                     PLAWLLAVIIGLWVLYRIIRGWIALADRRPMPVPD"
     gene            2231..3250
                     /locus_tag="AC16CX_04785"
     CDS             2231..3250
                     /locus_tag="AC16CX_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011864.1"
                     /translation="MKINRPTAGVPLCCFWVLVFSFLLSPFAFRLESAAHAADYPSRP
                     IRMIVPYPPGGSTDPTGRAFAAWLTEALGQQVVVDNRPGAGATIGHGLGAKATPDGYT
                     LLLGTSGGLATGPALATKLPYDPVRDFTPIGLGVYTPFLLVVHPGLPAANLKEFIALS
                     KTQPGGINFASVGVGTPNHLGAELLKVMAGFQFVHVPYKGGGPAVVDLIGGRTQALFG
                     GIPYTGPHVKAGRLRAIAIGHPKRMKTWPDVPAIAETLPGFSNTTWFGLLGPAGTPKA
                     VVNLINAEMRKAVANPGFVKHLEAIGLEPASSTPAEFHDMIRTELKRWTRVVKDAGIT
                     AEAAQ"
BASE COUNT          606 a         1163 c         1155 g          607 t
ORIGIN      
        1 ccgcgaccag tgcgatagca actcgccgaa gagcgcagtg ccataatccc ggcggctgcg
       61 ccccgtctcc gccccaacac caaccccctc gccgagatcc ggtgcgccgc cctcgcgcaa
      121 atggaacgat gagccggatg ggcttggccg gataggcctg tgcccatccc gccgctgcgc
      181 cgcataacag cgccgccaac acgattctag atatcgtttt catgaattct tcctctggct
      241 gattctacga tacgcggtgc cgcggcgaaa atcgtgatga gtgataggtg ataagtgata
      301 aggcccccgc agtgcggaag ttaccgactc ctatcaccta tcacttatca cccctcgaca
      361 gtcgcgcgct cacgccgtga gcgcttggat gtccaccgac gcggagccga tctcgcgcat
      421 caggaattcc ggcggcggct ggcgttccgc ctcggtgcgc tcggcgggcg gtttgacctg
      481 tgcttcgata taggcgccga gccgccgcgc gcgcgcgccg atgcgagtgc cgaacaggcg
      541 ccgctcgcga tcgtagcgtt cgagcgccgc ggcgaggtcc ccgcgcgcct cccggatggc
      601 gctcgcgagg cattgtgcgt cgagcgcggc cttggttaca cccatcccca cgtgcggccg
      661 cgcgacgaag gcggcatcgc cgagcagcac cacccgcccg agcgcgatcc gcggcgactc
      721 gaggtcgaaa atggcctgga agaacggctg cgcggtgcgc tcgacgatgg tggcgatctg
      781 gggcgcgagc agtgcgcggg cgtccgcttt gacatgcgcg acgacctcgg gcctgatgag
      841 tggcggcggg atcgagaccc cgtgacagcg gccggtggcg tcggtgcaca tccgcgcgag
      901 cgcctcgcgc tcctcggtcg gccggtacca cacgaagttg tagccgcggc ggcccgggtg
      961 cgtgtcgttg tcgcggccgg ggacgggata ggagagcatc atctcgcctt ccggcaggca
     1021 catcgaatag agctcgaaaa tttccgcgtg caccgcgggc ggaacgtcgc gctcatcgag
     1081 tacgccgcgc cacgcaatgt agccggcgta gcgcggctgg acgccgggca ggagctgctc
     1141 gcgcaccgtg gagcggatgc cgtcggcgcc aacgaggaga tccccgctct cgcgcgaccc
     1201 atcggtgaag acagcggtca cgccgtccgc ttcctgctcg acgcgctcga gggacattcc
     1261 gaagcgataa ctatcggcgg ggaacctgtc cttgagcagg cggtagatcc gcgcccagga
     1321 gctctggatc tgggacagct cgagccggtg ggtgagcttg ccgctcgagt cgaggcagat
     1381 ccgcttccgg acattgatgc cgatggattc gtccacccgc agccccaggc gccgcagcac
     1441 cgccagcagc tcctcgtggg tgccgatgcc ggcgccgcgg ctcgccaggt catcctcgac
     1501 gcgctcgaac acggcgacgt cccagccgat tgcgcgcagc cagttggccg cgaacagccc
     1561 gccgagcgag ccgccgataa cgagagcgcg tccctgagcg ctcataaagg cgtgatgagt
     1621 gatgagtgat gagtgatgag tgagaggact caactgcgtg ataagtgaaa agtgataaga
     1681 acggccggca ctgccggcct catactccga tcacctatca cctatcacct atcacgcatc
     1741 tgatattatc gtcgtcgttg ccaagaatat taccgctatg gaagccaact tgccccaggc
     1801 atcggtgtcc ccgccctcag atggaatggt gacgctcacg cacgtgctct acggcctgca
     1861 cggcttcagc gcgttgatgg gcgcgctggg gccggtgctg gtcgtgacgg cgttcctgac
     1921 cggttggccg tccatcattg ccatcatcat caactacgtg aagcgcggtg acgcgcgcgg
     1981 cacttggctc gattcgcatt tcggctggca gctccgcacg ttctggtacg ccgtgctgtg
     2041 ggtcgtgatc gcgggtctgg tgttcctcac tgtcatcggc atcccgctcg catggctgct
     2101 cgcggtgatc atcggcctct gggtgctcta ccggatcatc cgcggctgga tcgcgctcgc
     2161 cgaccggcgg cccatgcccg tgcccgactg acaaaggcgg aagtcgaaag tcgaaaggcg
     2221 aaaggactac atgaaaatca accgcccgac ggccggtgtg ccgctttgct gtttctgggt
     2281 tttggtcttt tccttcctcc tttcgccttt cgcctttcgc cttgagtcgg cggcgcacgc
     2341 cgccgactac ccttcgcgcc cgatccgcat gatcgtgccg tatccgcccg gcggctcgac
     2401 cgacccgacc gggcgcgcgt ttgccgcgtg gctcaccgag gcgctgggac agcaggtcgt
     2461 ggtggacaac cgtcccggcg cgggcgcgac gatcgggcac gggctcggcg ccaaggcgac
     2521 gcccgacggc tatacgcttc tgctgggcac ttccggcggg ctggcaaccg gtcccgccct
     2581 cgccacgaag ctgccgtacg acccggtcag ggatttcacg ccgatcggcc tcggcgtgta
     2641 cacgccgttc ctgctggtcg tgcatcccgg cctgccggcc gcgaacctca aggaattcat
     2701 cgcgctctcc aagacgcagc ccgggggcat caacttcgcc tcggtagggg tcggaacgcc
     2761 caaccatctc ggcgccgagc tgctcaaggt gatggccggc ttccagttcg tgcacgtgcc
     2821 atacaaaggc ggcggcccgg cggtcgtgga tttgatcggc ggccgcacac aggcgctctt
     2881 tggcggcatc ccgtacaccg ggccgcacgt gaaagccggc cggctgaggg ccatcgccat
     2941 cgggcatccg aagcgcatga aaacatggcc cgatgtgccg gcgatcgcgg aaacgctccc
     3001 gggattcagc aacacgacat ggttcggatt gctcgggccc gccggcacgc cgaaggcggt
     3061 ggtgaacctc atcaacgcgg aaatgaggaa ggcggtcgca aacccggggt tcgtcaagca
     3121 tctcgaggcg atcgggctcg agccggcgag cagcacaccc gccgaatttc acgacatgat
     3181 ccgaaccgag ctcaagcgct ggaccagggt cgtcaaggac gcgggaatca ccgcggaggc
     3241 ggcacaatag cgccgcttcc aagaggcgtg ataggtgata ggagctaagt gccgcgcgcg
     3301 ggcgggtttc ttatcactta tcacttatca cgcttttggt cggcacgtgc gatgaattcc
     3361 agctactgga ggtgatgcga atgatgatcg ttgattcgca ggtacatatt tgggcgcctg
     3421 attcgccgga gcgcccgtgg ccgaaaaacc gtacggtcaa ggcgcagcgc gacgtgccgc
     3481 tcggcaagga cgagctgctg cgcgaaatgg acgccgccgg cgtcaatcgc g
//
LOCUS       DBNGWZ010000162         5977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1401437, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000162 DBNGWZ010000000
VERSION     DBNGWZ010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1401437"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16CX_04790"
     CDS             <1..389
                     /locus_tag="AC16CX_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011865.1"
                     /translation="PVDESNSLRSVGTSRDQPDVLIMDNKYESVSAVRSYLLSRFPDC
                     AVDDRFDFDTRGQVFTIERGARVFVVRASHEFLDDVDSSEIGKLLLAWNLDEALHTSS
                     DVLVVTRHVLSPRPSHTVPRLISRIA"
     gene            complement(1466..2437)
                     /locus_tag="AC16CX_04795"
     CDS             complement(1466..2437)
                     /locus_tag="AC16CX_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3011866.1"
                     /translation="MKPASAAVLSLAQLLEQQPAAPPACLVEPGLLPAQGILFVGGEP
                     KVGKSLLVANVALSLAAGTDRIGFPIPAPRRVLVCQFELPVPQFVSRLVTMRRALGAA
                     ADQHLLVDTRAAGHLLSAAEGLGHFLAAAKAASAEVIVLDPLYSAHDQDENDTRAMAA
                     LCQSLLRLRDASHAALIVVHHVRKSIGRHEIGSAFRGSSALHAVGDSYLLVTRPSPQL
                     ATVELRFQFRYAAPQPPRLLELNPQTLWFSSTASQPVPAALRRKVEKSDVVRAMANVG
                     GPARYTQLRGEIMNLTECSKRTAQLAITEACREGRVVQADGQYRLPL"
     gene            complement(2434..3234)
                     /locus_tag="AC16CX_04800"
     CDS             complement(2434..3234)
                     /locus_tag="AC16CX_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3011867.1"
                     /translation="MVEAFFGFKKTPFGDRPDPKQLFPSQAWNQVKARLQFLVEHRGA
                     GLLTGEVGSGKSTAARSFTAALNPNLFKVMYLHWTAGSALDLLRQVALELDLEPAHYR
                     GDLARQISQAIVRLNQSKKQHPLLICDEAQLLCHPALEQLPLLLNFDMDSSRYLTLLL
                     VGQPLLRRTLSLQMHEPLRQRIAVQFHLEGLSREELDAYLAHQLKAAGVTQPLFDDTA
                     RQALYQATKGILRKVNKLALTALRLASVRKASLVDEPILLEAATEALL"
     gene            complement(3228..4541)
                     /locus_tag="AC16CX_04805"
     CDS             complement(3228..4541)
                     /locus_tag="AC16CX_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HIR3011868.1"
                     /translation="MDDKAEKIALFRYGLVAPLVLEPLARGELTQRAEEIASRPYDIP
                     DSKRRTVSVDTLLEWAKRYRNGGLDALGPKARQDRGLSRTITPQLAGLIERLKHENPH
                     RTGTTLLRELALSSGQDEPPLSASTLYRFLKQRGLSERQLLAPPAHKKFEAELANQIW
                     QADMLFGPWVARPGGGRRQVFLHATLDDASRLIPHAQFYASQGLDAALDCLRQAIAAR
                     GIPIRLYIDNAKMYRSPQLARIAASLGALIIHSRPYQPEGRGKIERCFRTVRDQFLAN
                     LDPKRLLSLEELNDRLWAWIDGVYHRSEHSALGTTPLLRWQRDIEHVRQLPPGTDLRR
                     LFFHRLDRLVRRDSTFLLRNRLYEAPPHLAGRTIEARFDPLDAAEVEIWSEGKLQAAA
                     RPLDPVVNGRLPFAKPTEAPKPELTGINFVELLNNKKDDDEEPPW"
     gene            complement(4617..5222)
                     /locus_tag="AC16CX_04810"
     CDS             complement(4617..5222)
                     /locus_tag="AC16CX_04810"
                     /inference="COORDINATES: protein motif:HMM:NF040103.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HIR3011869.1"
                     /translation="MQILHPFAGSIQQYAEAISDPDRYRPDHCPQCEAKQPLTGHGFY
                     SRTLADPVFDGVIRVRRYLCRLCKRTVSLLPEFALPWLRFSITVISLFLVARLLQGLT
                     LAAAARAACQAAMPYQRGQFWIRRFRTQAPALTLALAPLAAPAAAPDFVARALGMLES
                     IGWIAAHRFLFSALRAHLLGWPHFLAPAGRRATLVPACPVP"
     gene            complement(5352..>5977)
                     /locus_tag="AC16CX_04815"
     CDS             complement(5352..>5977)
                     /locus_tag="AC16CX_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011870.1"
                     /translation="TDECVIPGSRCVVIEHQGGRASASVGNLMQSFTAAPYRNRKARL
                     SASVRVVRDVSAAGECKAQMWFRVDLPNKVMSAFDDMDRRPIISSEWKTYQIAGRIDE
                     EAEGINFGIMLFGSCKAFVDEMSFEALGTVRPPFRDIAPRAEDALEVEWLLKNALPIR
                     SIDPGDDDFSDLMPLNGPRFSPPSRGAFLKLSSQGMGATMRTGWSAV"
BASE COUNT         1104 a         1799 c         2030 g         1044 t
ORIGIN      
        1 cgcccgtgga cgaatcaaat tctttaagga gcgtcgggac atcgcgcgat cagcctgacg
       61 tattgataat ggacaacaag tatgaaagcg tgtctgcggt gagatcctat ttgctctccc
      121 ggtttcccga ttgcgcggtc gacgacagat tcgatttcga cacacggggc caggtcttca
      181 ctatcgagcg cggggcgcgt gtcttcgtag ttcgggcctc acacgagttt ctcgatgacg
      241 ttgactcatc cgaaatcggc aaactgcttc tggcatggaa cctggatgaa gcactccaca
      301 caagctccga tgtgctcgtt gtaacgcgcc acgttctttc ccctcgaccg tctcacactg
      361 ttccccggct gatttctcgg attgcttgac tgctcttcgc gttccgcggt gaagggagtg
      421 ggataggata ctcgcccgtg taggcaggac cggtatcccg ctctacatca gaggaaaacc
      481 tattcggagc gcccggcaaa agcggccggg cgatcttcgc attttatagc ccagggcctc
      541 cgctccgaag ctttccaaag aaatcaaggg cttttttctc gcacgaacaa cgggtggtcc
      601 tggccgcgcg gatgtccgct gggcggggtt gatttggcaa tgtggtcatc atcgcgggcc
      661 gaggctgctg caggcgccta cgttccggga acgccgtgtg tcggccgcca tgccatcatt
      721 tacagtcaga aggtgtgatc tgaaggctgc atcggtacac gcgatgaatc aaatccccga
      781 gggcttcgaa ctcgcacgcg ttcgtaatct cccggccaac ataccacccc tcggcgctgc
      841 cggctggaac agtggcttcg acgcgaagga cgcaaggaaa agcccggacg gagacccgaa
      901 ccaagtcttc cttgaacgcc cgaacactcc tggcaccaga ggcacgatag gcgggccagt
      961 ccgtctcctt ccgcgacctg agattgacac cagggacgtg gcggaaggac agcagcgcct
     1021 cccacacgtc ttggccaaga gcctcgcttg acgagttcaa tgagattgaa gattggcaca
     1081 tcctgctcga cgtagaggcc tttttcgacg gtcatgttcg ggcagacgat tatcttctct
     1141 gatgcgcgga ggaagtatac ggtcacaacc ggatcgtctc cctcgctttt cgcgatggcg
     1201 gccaatgccg gcactgggaa tcggctgaaa tcgcggctca tttccatact ctacggccaa
     1261 acggcagggt ccgttaccga ttggattcag ggtgagcaaa atgcgcccgg tttgagatgg
     1321 aaaactggaa gaaccctccc gcgctccatc ccgcgcggga gggttcggtg agccgatttc
     1381 ttcggagcca gctccgtcgt atcggccctg ggcatccgcc cgggcgcgac cccggacgga
     1441 caatcgatgt ccaccgccga atacactaca gcggcaggcg atactggccg tcagcctgca
     1501 cgacccggcc ctcgcggcag gcctcggtga tggccaactg tgcggtgcgt ttggagcact
     1561 cggtgagatt catgatttca ccgcgcagtt gggtataacg cgctggtccg ccgacgttcg
     1621 ccatcgcccg cacgacatcg ctcttctcaa ccttacgccg cagcgcggcg gggacgggct
     1681 gggacgcggt ggaagagaac cagagtgttt gcgggttcag ctccagcaac cgcggcggct
     1741 gcggagcggc atagcggaac tggaaacgca gctcgacggt ggccagttgc ggcgacggcc
     1801 gggtgacgag caggtagctg tcacccaccg cgtgcagggc gctggagccg cgaaaggcgc
     1861 tgccgatctc gtggcggcca atcgatttgc gcacgtggtg caccacgatg agcgcggcgt
     1921 gcgaagcatc gcgcagccgc agcagcgact ggcacagtgc cgccatggcg cgcgtatcgt
     1981 tctcgtcctg atcgtgcgcc gaatagagcg gatcgaggac gatgacttcg gccgaggcgg
     2041 ccttggccgc cgccaggaaa tgaccgaggc cctcggccgc gctgagcaga tgtccggcgg
     2101 cacgcgtgtc caccagcaga tgctggtcgg cggcggcgcc cagggcgcgg cgcatcgtaa
     2161 caagccggct gacgaactgc ggaacgggca gctcgaactg gcagacaagc acgcgccgcg
     2221 gcgccgggat gggaaagccg atccggtccg tgccggcggc cagcgagagc gcgacattgg
     2281 ccaccagcag cgacttgccc accttgggtt cgccgcccac gaagagaatg ccctgcgccg
     2341 gcaacagccc gggttccacc aggcaggccg gcggcgccgc cggctgctgt tccagcagtt
     2401 gagccagcga cagcacagcc gcggatgcgg gcttcacagc agcgcctccg tagctgcctc
     2461 cagcaggatc ggttcgtcca ccagggacgc cttgcgcacg gaggcgagcc gcagcgccgt
     2521 cagcgccagc ttgttgactt tgcgcagaat gcctttggtc gcttggtaga gggcctgccg
     2581 ggcggtgtcg tcaaacagcg gttgcgtcac tcccgccgcc ttgagctgat gggccagata
     2641 ggcatcgagt tcctcgcgcg agagcccttc gaggtgaaac tgcacggcga tgcgctggcg
     2701 caacggctcg tgcatctgca acgacaaggt gcgccgcagc agcggctggc ccacgagcag
     2761 cagcgtcaga taacgcgagg aatccatgtc gaagttgagc agcagcggaa gctgctccag
     2821 tgccggatgg cagagtaact gggcctcatc gcagatgagc agcgggtgct gcttcttgct
     2881 ctgattgagc cgcacgatgg cctgcgagat ctggcgggcg agatcgccgc gatagtgcgc
     2941 cggctccaga tccagttcga gcgccacctg gcggagcaag tccaaggccg agccggccgt
     3001 ccagtgcaga tacatcacct tgaacaagtt gggattgagc gcggcggtga agctgcgcgc
     3061 ggcggtggac ttgcccgagc ccacttcgcc ggtgagcagg cccgcgccgc gatgctcgac
     3121 gagaaactgg agccgcgcct tgacctgatt ccacgcctgc gatgggaaca actgtttcgg
     3181 atcggggcgg tcgccgaacg gggtcttctt gaaaccgaag aaggcttcta ccatggcggc
     3241 tcctcgtcgt cgtccttttt gttattgagc agctcgacga agttgatgcc ggtgagctcg
     3301 ggcttggggg cctcggtggg tttggcgaaa ggcaaccgcc cgttgacgac cggatcgagc
     3361 gggcgcgcgg cggcctggag ttttccttcg gaccagatct cgacctcggc agcatcgagc
     3421 ggatcaaaac gcgcctcgat ggtgcggccg gccagatgag gcggagcttc atagaggcgg
     3481 ttgcgcagca ggaacgtcga atcccggcgc acaagccggt cgaggcgatg gaagaagagc
     3541 cgtcgcagat cggtgccggg cggaagctgg cgcacgtgct cgatatcgcg ctgccagcgc
     3601 agcagcggcg tggtgccgag ggcgctgtgt tcggagcggt ggtagacgcc gtcgatccag
     3661 gcccacaggc gatcgttcag ctcctcgagc gaaagcaggc gtttgggatc gagattggcg
     3721 aggaactggt cgcgcacggt tcggaaacag cgctcgatct tgccgcgccc ctcgggctgg
     3781 taaggccgcg agtgaatgat gagcgcgccg agcgaggcgg caatacgggc cagttgcggc
     3841 gagcggtaca tcttggcgtt gtcgatgtag aggcggatcg gtatgccgcg ggcggcgatg
     3901 gcttggcgca ggcagtcgag cgcggcgtcg agtccctggc tggcataaaa ctgcgcgtgc
     3961 ggaatgaggc ggctggcgtc gtcgagcgtg gcgtgcagga agacctgccg tcggccgccg
     4021 ccgggccggg cgacccaggg gccgaacagc atgtcggcct gccagatctg gttggcgagc
     4081 tcggcctcga actttttgtg tgccggcggg gccagcagtt gccgttccga gaggccgcgc
     4141 tgtttgagaa agcgatagag cgtggaggcc gacagcggcg gctcgtcctg gccggaggag
     4201 agggccagct cgcgcaacag cgtcgtgccg gtgcggtgcg ggttttcgtg cttgaggcgt
     4261 tcgatgaggc cggccagttg cggtgtgatg gtgcgcgaga ggccgcggtc ctggcgcgct
     4321 ttgggaccga gggcgtcgag gccgccgttg cggtagcgct tggcccattc cagcagcgta
     4381 tcgacgctga cggtgcggcg tttcgaatcg gggatgtcgt agggacggga tgcgatttcc
     4441 tcggcccgct gggtgagctc gccgcgcgcg agcggttcga gcacgagcgg cgccaccagg
     4501 ccgtagcgga agagcgcgat tttttcggct ttgtcatcca tcggtgtttt ctctcccgcc
     4561 ccaaagccta aggcgggaag ccctgccggc tccaggcaaa tgctgtgtgt gcgccttcag
     4621 ggaaccgggc acgccggcac cagcgtggcg cgccgtccgg ccggagcgag gaagtgcggc
     4681 cagcccagca ggtgagcgcg cagcgcggag aacagaaagc ggtgagcggc gatccagccg
     4741 atggattcga gcatgccgag ggcgcgggcg acaaagtccg gcgcggcggc cggcgccgcc
     4801 agcggcgcca gcgcgagggt gagtgcgggc gcctgtgtgc gaaagcggcg aatccagaac
     4861 tgcccgcgct gatagggcat ggcggcttga caggccgccc gtgcggcagc cgccagggtg
     4921 aggccctgaa gcaggcgcgc cacgagaaac agcgagatca ccgtgatgct gaagcgcagc
     4981 cacggcagcg cgaactcggg cagtaaagaa accgtgcgct tgcagaggcg gcacagataa
     5041 cggcgcacgc ggatcacgcc gtcaaagacc ggatcggcca gtgtgcggct ataaaagccg
     5101 tggccggtta gtggctgttt ggcctcgcat tgcgggcaat ggtccgggcg gtaacggtca
     5161 ggatcggaga tcgcttcggc gtactgttgg atggagccgg cgaacgggtg taggatttgc
     5221 atgggcgcct ttccgggagt ggtgaagact ccctgattgg gtgtagggga gggcccggct
     5281 gccattcagc caggctctcc gccccgccat cctactccgg aaacgcaaac cgcgcgcgat
     5341 cccaccgccg ttcaaactgc actccatccg gttcgcatgg tcgcgcccat tccctgcgaa
     5401 ctcaatttga gaaaagcgcc tcgcgacgga ggggaaaatc gcggtccgtt cagcggcata
     5461 aggtcggaga aatcatcgtc tcccgggtcg atggagcgaa tcggcaacgc gttcttcaac
     5521 agccactcga cttctagcgc gtcctcggcg cgaggcgcaa tatcgcggaa cggcggccga
     5581 acagtgccga gcgcttcaaa ggacatttca tcgacgaacg ccttgcacga cccaaataac
     5641 atgatgccga aattgattcc ttcggcctct tcgtcaatac ggccggcgat ttggtaggtc
     5701 ttccactcgg atgaaatgat tgggcgtctg tccatgtcgt cgaaggcgct catgaccttg
     5761 ttgggaagat ccacgcgaaa ccacatctgg gccttacact cgccggccgc tgaaacgtca
     5821 cggaccacgc gaaccgatgc gctcaatcgc gccttccgat tgcgataggg agcggcggtg
     5881 aacgattgca ttagatttcc cacgctcgct gatgcacggc cgccctgatg ctcgatcacc
     5941 acgcaacggg agccgggtat cacgcattcg tctgtga
//
LOCUS       DBNGWZ010000163         7360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1148691, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000163 DBNGWZ010000000
VERSION     DBNGWZ010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1148691"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1096
                     /locus_tag="AC16CX_04820"
     CDS             104..1096
                     /locus_tag="AC16CX_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011871.1"
                     /translation="MSVRFAINRIWRAALALVLAGPVASIAAGPAASYPIRPVRVVVP
                     FTPGGQPDIIARMIAPKLTEFLGQQVIVDNRPGAGGMMGSRIVASANPDGYTLLSISA
                     AHVVTPAVRANPGYDTIKDFAGVTITGNAAYYLVVPPSLEVKSVKELIALAKAKPGQL
                     NFTSAGRGSATHFGGEMFKSSAGIDVVHVPHKGIPEALTDVMTGRVHFFMAPFGSTVG
                     LIKDGKLRALAVSTEKRTRMNPEIPTIAEAALPGFRYTSWAALFAPAKTPRAIIDKLN
                     REVARVLKLPDVEQRLHAIGIEPAPSTPAQLEKFVAEQLAAAKQLAQKAGIKPE"
     gene            1280..1738
                     /locus_tag="AC16CX_04825"
     CDS             1280..1738
                     /locus_tag="AC16CX_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011872.1"
                     /translation="MATRKKAKAKRKKAPARRPAARRKTAKAGKKPAKKAPVRRAPAR
                     KAAPARPPKPAARPETAALPVQLPPPGERIGVVTHYFSHLAVAVVKLETATLRVGDMI
                     HFRGHTTDFGQRVESLQVNHAPVTEVGPKDDFGMKVREHVREHDVVYKVG"
     gene            1892..2650
                     /gene="pcm"
                     /locus_tag="AC16CX_04830"
     CDS             1892..2650
                     /gene="pcm"
                     /locus_tag="AC16CX_04830"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HIR3011873.1"
                     /translation="MTRAAFRFQHVVAVFALLAFSGAIAQEANEFAEMRRLLAEGIEK
                     GVNQARKHTGGNPIDRRVIAAMGKVPRHEFAPPELRSYAYLDRPLPVGPGPDSTISQP
                     SLVAVMTDLLGIKHGEKVLEVGVGGGYHTAILAEMTPAVFSVEFHAKVAEAAKRTLER
                     LGYGGVKVNVADGYYGWRPNAPYDAILVRMAIPHVSSALLDQLRPGGRLVAPVGPADG
                     IQVLVLLNKGRNGDVTETRIMPVRFRALPGGVRL"
     gene            2782..3690
                     /locus_tag="AC16CX_04835"
     CDS             2782..3690
                     /locus_tag="AC16CX_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011874.1"
                     /translation="MAQPYPVKPVRLIVAFPAGGGSDIVGRALAQQLTDILGQPVIVD
                     NRGGAGGVIGTELGARAAPDGYTLLIGSSGGFAINPHLNPRLPYDPTRDFAPVGLVTR
                     LTFVLDVHPSVPAKSVKEFVALAKSRPGRLNFGSSGQGATAHLATELFMSLARVRMTH
                     VPYKGAAPAMTALLSGEVDVLFDSMPTTLPHARSGRIRPLAVTTLQRSALMPQLPTLD
                     EAGVKGYELANWMGVFSPAGTPRAVVDRLNSAINAATRRPELKERLSSQGAEPLSGTP
                     EDLEAYLRRELDKYGRIIREAGVKAE"
     gene            4028..4447
                     /locus_tag="AC16CX_04840"
     CDS             4028..4447
                     /locus_tag="AC16CX_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011875.1"
                     /translation="MRFISMTSAVLAVLTLITPVSTLAAEKSLGQQEFESKCASCHGI
                     GAKGGGWMAKYLSHRPPALTQLTKKNGGVFPFDYAYQIIEGRKDVELHGPREMPVWGA
                     VLPTEIERQNLGLCVADEEVARRRIRALIEYISQLQE"
     gene            complement(5276..6247)
                     /locus_tag="AC16CX_04845"
     CDS             complement(5276..6247)
                     /locus_tag="AC16CX_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011876.1"
                     /translation="MYWGSQWARTVGLALTLLACVAVAPAASWPEKPVRIIIPWPPGG
                     STDIVGRLLAADLGARLKQQVIIDNRAGAGSIVGLQVATAAPPDGYTFMKTSTAYGFL
                     IDKPKVPVDLVNSFAPVALIGFGDSALVVHPSFPVRTVKDLIDLAKKRPGEIFYASSG
                     IGGFPHMNTELFKLKTGVNLVHVPFKGGGSAIADVVAGHTQLQIGSLVTVMPHVQSGR
                     LRLIALGSPRRSPKYPDVPTIAETVPGYESRIWWGVFAPPKTPPDIIARFHAETMEVI
                     SSPAFQKRLDEQGGAVVKMSSAEFGRLMVSEQNKWLEVIRAAGIKGE"
     gene            complement(6536..>7360)
                     /locus_tag="AC16CX_04850"
     CDS             complement(6536..>7360)
                     /locus_tag="AC16CX_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011877.1"
                     /translation="RMLADKFASSWGANVVVDNRTGASGIIGTDIVAKAAPDGYTLGF
                     VALSFAINPSIHKLPYDAGRDFTYITVVASNPLALVVNSNVPAKSVKELIELAKAKPD
                     GLSFASSGTGTSPHMSAELFKLLAGVRILHVPYKGSTAAHPDLIGGRVSMMFDPVAAI
                     LPHIKAGRVRPLGVTSKSRSAELPDVPPIADTVPGYESTSWVLIMGPAKMPAAVADRL
                     HKETVRALAFPDVKERLARMGSDIVGSSPDEARRFIRAELEKWAKTVKAAGITSGQ"
BASE COUNT         1366 a         2389 c         2297 g         1308 t
ORIGIN      
        1 gcgcgctgtg attcgaaggt ttcgacttgg aacttggaac tttgaacaag atctttgtca
       61 ccactcacga ctcacgcaat tcataaaaaa gaggagggaa agcatgtctg ttcgctttgc
      121 gatcaacaga atatggcggg ctgcgcttgc tctcgtgctc gccggcccgg tggcctcgat
      181 cgctgccggc ccggctgcga gctacccgat ccggccggtc cgggtcgtcg tcccgttcac
      241 gccgggcggc cagcccgaca tcatcgcgcg catgatcgcg ccgaagctga ccgagttcct
      301 ggggcagcag gtcatcgtgg acaaccgccc tggggcgggc ggcatgatgg gctccaggat
      361 cgtggcgtcc gccaatcccg atggctacac cctgctgtcg atttctgccg cgcacgtggt
      421 gactcccgcc gtgcgcgcca atcccggcta cgacacgata aaggacttcg ccggcgtcac
      481 gatcaccgga aacgccgcct attatctggt ggtcccccct tcgctggagg tgaaatcggt
      541 gaaggagctg atcgcgctgg cgaaggccaa gcccgggcag ctcaacttca cctccgccgg
      601 gaggggcagc gccacgcatt tcggcggcga gatgttcaag agcagtgccg gcatcgacgt
      661 ggtgcacgtg ccccacaagg gcatccccga ggcgcttacc gacgtgatga cgggccgcgt
      721 gcacttcttc atggcgccgt ttggctccac tgtcggactg atcaaggacg ggaaactgcg
      781 cgcgctcgcg gtctccactg agaagcgcac gcgcatgaac ccggagatcc cgaccatcgc
      841 agaggccgcg ctgccgggct tccgctacac ctcgtgggcc gcgctgtttg cgccggcgaa
      901 gaccccgcgc gccatcatcg acaagctcaa ccgcgaggtc gcgcgcgtcc tgaagctccc
      961 cgacgtcgag caacgcctgc acgccatcgg catcgagccg gcgccgtcca cacccgccca
     1021 gctcgagaag ttcgtcgccg agcagctcgc cgcggcgaag cagctggcac aaaaggcggg
     1081 aatcaagccc gagtgacgag cgccgggtgt cgggtgtcga atgaggagcg gaaatcacct
     1141 tgttacctat ttctccctcg acactcaatc ctcgacactc tccctcgtcc ctgctatttg
     1201 atgcctgccc gcggcggggc taagatttga cggtcggggt cactttctat gggcgggtcc
     1261 gcccgccggg aggctgaaca tggcgaccag aaagaaagcg aaggcgaagc gcaagaaggc
     1321 gccggccagg cggccggcgg cgcgcaggaa gaccgcgaag gccgggaaga agccggcgaa
     1381 gaaggcgccg gtccggcgcg ccccagcccg gaaagccgcg ccggcgaggc cgccgaagcc
     1441 ggcggcaaga ccggagacgg cagcgctgcc cgttcaactt cccccgccgg gcgagcgaat
     1501 cggcgtcgtg acccattatt tcagccatct cgccgtcgcg gtcgtgaagc tggaaacggc
     1561 aacgctgcgc gtcggcgaca tgatccactt ccgcggacac accaccgatt tcggccagcg
     1621 ggtcgagtcc cttcaggtca atcatgcgcc ggtcacggaa gtgggcccga aggacgattt
     1681 cgggatgaag gtgcgcgaac acgtgcgtga gcacgacgtc gtatataaag tcggatgaag
     1741 cgtcaaaggc gacaactgcg tgataagtga tgggtgataa gaacggccgg caccgccggc
     1801 cttgactcct atcgcccatc acctgtcgcg ttttttgcct cagcacctat cacttatcac
     1861 ctatcacgct tttccctcat cccctggttc catgacccga gcggctttcc ggttccagca
     1921 cgtcgttgct gtctttgccc tgttggcgtt ctcgggcgcg atcgcgcagg aagcgaacga
     1981 gttcgcggag atgcgccgtc tcctcgccga aggaatcgag aagggtgtca accaggcgcg
     2041 caagcacacg ggtggaaatc cgatagaccg gcgcgtgatc gcggcgatgg gcaaggtgcc
     2101 gcgccacgag ttcgcgccgc ccgaacttcg gtcatatgcc tatctcgacc ggccgcttcc
     2161 cgtcggaccc ggtcccgact cgacgatctc gcagccctcg ctcgtcgcgg tgatgaccga
     2221 cctgctcggg atcaagcacg gggagaaagt gctcgaggtc ggcgtcggcg gcggctacca
     2281 taccgcgatc ctcgccgaga tgactcccgc ggtgttcagc gtcgagtttc acgccaaggt
     2341 cgcggaggcc gcgaagcgca cgctcgagcg gctcggctac ggcggcgtca aggtcaacgt
     2401 cgccgacggc tattacggct ggcgtccgaa tgcgccatat gacgccatcc tcgtgcgcat
     2461 ggcgatcccc cacgtgtcct ccgcgctcct cgaccagctc aggccgggcg ggcgcctcgt
     2521 cgcgccggtc ggccccgccg acggcatcca ggtgctggtc ctcctcaaca aggggcgtaa
     2581 cggagacgtg acggaaaccc ggatcatgcc ggtgcgcttc cgcgcgctgc cgggcggcgt
     2641 caggctgtga taagggatga gggcggaggg atgagggatg aggataaagg cgagaacgca
     2701 agagctaaga gagccaggct ttcattcctc cttcatcctt ctgccttctg ccttggctgt
     2761 gccatcgcag ctggcgcagc ggtggcgcag ccgtaccccg tcaagccggt gcgcctgatc
     2821 gtcgcgttcc ccgccggcgg cggcagcgac atcgtcgggc gcgcgctcgc tcaacagctt
     2881 accgacatcc tcggccagcc ggtcatcgtg gacaaccgcg gcggcgccgg cggcgtcatc
     2941 ggcaccgagc ttggcgcgag ggccgcgccc gacggctaca cgctcctcat cggcagctcg
     3001 ggcggctttg ccatcaaccc gcacctcaac ccccggcttc cgtacgaccc gaccagggat
     3061 ttcgcgccgg tcggcctggt cacgcggctg acgttcgtgc tggacgttca tccgtcggtg
     3121 cccgcgaaat cggtcaagga gttcgtcgcc ctcgcgaaat cgcgtcccgg acgtctcaat
     3181 ttcggctctt ccgggcaggg ggcgaccgcg catcttgcga ccgagctgtt catgtcgctt
     3241 gcccgcgtac ggatgacgca cgttccgtac aaaggcgcgg cccccgccat gaccgcgttg
     3301 ttgagcggcg aggtagacgt gctgttcgat tccatgccga ccacgctgcc gcacgcgcgc
     3361 tcggggagga tccggccgct cgcggtgacc accctgcagc gctcggcgct gatgccgcag
     3421 ctgccgacgc tggacgaagc cggtgtgaag ggctacgagc tcgcgaactg gatgggcgtt
     3481 ttctcgcccg ccggcacgcc gcgcgccgtg gtcgatcggc tcaactccgc tatcaacgcc
     3541 gcgacccggc gacccgagct gaaagagcgg ctgtcgagcc agggcgcgga gccgctctcc
     3601 ggcacgcccg aggaccttga ggcctacctg cgcagggagc tggacaaata cggaaggatc
     3661 atccgcgagg cgggcgttaa ggctgagtga cgtaaagctg aaccgccaag cccgccaagg
     3721 acgcaaagta ttacaaataa aagctacgaa tctccacagt ccattcacca ttcactattc
     3781 accattcacc gcttttgaga ggccgcaaag acaacgccaa gagtggtatt cgggacacat
     3841 ccctggtagc cgatggcccc gtgtcgcgaa ccgtcgtcgc gacgtactct gacaggcagc
     3901 gtccgaattc gccaccttga gcggtcattc gccctaatgt aaaaccatgt caaaaaaact
     3961 ggtttcaaaa aaatcgccct atttggccaa cgagtttccc tgaaccaacc ggaaaaggag
     4021 agcggacgtg agattcatat ccatgaccag cgccgtattg gcggtattga ccttgattac
     4081 tccggtcagc acgctggccg ctgagaaatc ccttggccaa caggagttcg aaagcaaatg
     4141 cgcgagctgt catggaatcg gcgcaaaggg gggcggatgg atggcaaaat atttaagtca
     4201 tcgcccccct gcgcttaccc agctgacaaa aaagaatggc ggggtgtttc cattcgatta
     4261 cgcttaccaa ataatcgagg gcagaaagga cgtggagctg cacggcccgc gggagatgcc
     4321 cgtctggggc gccgttcttc ccacggaaat agaaaggcaa aatctcgggc tgtgtgtcgc
     4381 cgacgaggag gtcgcccggc gccggattcg cgcgctgatc gagtacatct cacagcttca
     4441 ggaataacgg gcggccaggc atcaacgata cagtgatcga cggctactgg ccgtgaccgg
     4501 acgccagcgg ccagagaaaa tctgagttat tacaagtcaa tttccggggt tccccatccc
     4561 agagcgcgac cagtatcccc cttggcgttg tcttcgcgat cttgattcgg tgactcggga
     4621 tcgcggctcg cggatgggcc ccgcgcggtg cgcgggaggc ttatcaccca tcactcatca
     4681 cggtcttatt agccggcacg ctttcgaatg tcgaaataga gagtgtgacc agccattttc
     4741 gagatacgag ccctttctgt accgaccaag acgtacagag cgttcaggac cgcgaagcgt
     4801 tcggttagaa atctggatcg cttgcaggcg gcaaaagcct cagcggaagt cactgcacgc
     4861 ttgccgcgca ggcgcctgac catgtaatca acgcccttac gcgcgactac cgtgaagttc
     4921 ccattggtcc ccttccctgg ggagaagaga acatagtgtt cgctgctttc agccacgtgg
     4981 tagttccgct tcgcggaccg gaccaggcca cagtagcgga gaccagagat aagggaccgg
     5041 tagttcatat tttcctccct acgtgaaaat tccgtttgcc ggctaacttt gaagaggaca
     5101 gccgaaaagc gtcttatcag gcggcattct gcgccgcgag gtgcgcgcct tcaagccaaa
     5161 gtgacagttg gcagccgtca gttttcagcc gtcagccgtc agtaaacaac gactgcaaac
     5221 tgcctactgc acttcttcct tcttccttct tccttcttcc ttctgccttg ctccgtcact
     5281 cgcccttgat gcccgctgcc ctgatgacct cgagccactt gttctgctcg gacaccatga
     5341 gcctgccgaa ctcagcggag ctcatcttca cgaccgcgcc gccctgctcg tcgagccgct
     5401 tctgaaacgc cggcgaggaa atcacctcca tggtctcggc gtggaagcgc gcgatgatgt
     5461 caggcggcgt cttcggcggc gcaaagacac cccaccagat ccgagactcg tatccgggca
     5521 cggtctccgc gatcgtcggc acgtcaggat acttggggct gcgccgcggg ctgccgagcg
     5581 caatcagcct cagccggccg ctctggacat gcggcatcac cgtgaccagc gagccgattt
     5641 gcaactgggt atgcccggcc acgacgtcag cgatcgccga gccgccaccc ttgaacggca
     5701 cgtgcaccag gttcacgccg gtcttcaact tgaagagctc cgtattcatg tgcggaaacc
     5761 cgccgatgcc cgacgaggcg tagaagatct caccgggcct tttcttcgcg aggtcgatca
     5821 ggtccttcac cgtcctcacc ggaaacgacg gatggacgac gagcgcgctg tcgccgaatc
     5881 cgatcagcgc gaccggcgca aatgaattga cgagatcgac cggcaccttc ggcttgtcga
     5941 ttagaaaacc gtacgcggtg gaggtcttca tgaaggtgta gccgtccggc ggcgcggccg
     6001 tcgcgacctg caagccgacg atgctgccgg cgccggcccg gttgtcgatg atcacctgct
     6061 gcttgagccg ggcgcctaga tccgccgcca gcagccgccc gacgatatcc gtgctgcccc
     6121 ccggcggcca gggaatgatg atgcgtaccg gcttctcggg ccaactcgcg gccggggcga
     6181 cggccacgca agcgagtaac gtcagggcga gcccgaccgt ccttgcccat tgcgatcccc
     6241 agtacatgac gctcctcccg cggtggtttt cggggtggac gttaatgtag ccggaaaaat
     6301 cgcgctgcga gaaagtcggg gcgcagcaat ggtgataggc gcaatagagg gtctccaggg
     6361 cccgccttcc cggcgattgc cggaacgatt cgagccttgt gcgccgtgag gcgtacgcct
     6421 tcaagccatt cttgatgggt cgtaactcgt gacttgtgac cggatcggaa attgacagtt
     6481 ggcggccgtc agctcttggg tcgacccacg actcacgaat cacgactcac ggccgttatt
     6541 ggccgctcgt gattcccgcc gccttgaccg tcttcgccca cttctccagc tcggcgcgga
     6601 tgaaacggcg cgcctcgtcc gggctgcttc cgacgatatc ggagcccatc cgcgcgaggc
     6661 gctccttcac gtcgggaaac gccagcgcgc gtacggtttc cttgtggagc cggtcggcga
     6721 ccgctgccgg catcttcgcc ggccccatga tcagcaccca cgacgtggat tcatagcccg
     6781 gcacggtgtc ggcgatcggc ggcacgtcgg gcagctcggc cgagcggctc ttcgaggtga
     6841 cgcccagcgg gcgcacgcgc ccggccttga tatggggcag gatcgcggcg accgggtcga
     6901 acatcatgct cacccggccg ccgatcaggt cggggtgcgc cgccgtgctg cccttgtacg
     6961 gcacgtggag gatgcgcacg cccgcgagga gcttgaacag ctccgccgac atgtggggcg
     7021 aggtgcccgt gccgctggaa gcgaatgaca ggccgtccgg cttggccttg gcgagctcga
     7081 tcagctcctt caccgatttc gcgggcacgt tgctgttgac gaccagcgcc agcgggttgg
     7141 atgcgacgac cgtgatgtag gtgaaatcac ggcccgcgtc atacggcagc ttgtggatgc
     7201 tcgggttgat cgcgaaactc agcgccacga agcccagcgt gtagccgtcg ggggcggcct
     7261 tcgccacgat gtcggtgccg atgatgccgg acgcgccggt gcggttgtcc accaccacgt
     7321 tcgcgcccca ggacgacgcg aatttgtcgg cgagcatgcg
//
LOCUS       DBNGWZ010000164         6824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_142164, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000164 DBNGWZ010000000
VERSION     DBNGWZ010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_142164"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16CX_04855"
     CDS             <1..194
                     /locus_tag="AC16CX_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011878.1"
                     /translation="ESRTGLYLLMGKAVRPIGRQKNGFEKAHNATMDRMARDPAGYAP
                     GNLVAYCNSKEFRVDLSEP"
     gene            523..762
                     /locus_tag="AC16CX_04860"
     CDS             523..762
                     /locus_tag="AC16CX_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR3011879.1"
                     /translation="MKDVKVTELRQNLPEYLAKIRRGERVRVTSRGKVIAEIAPPSAS
                     RKDAEAARARLRGSVLKYERPTEPAIDPGEWDANR"
     gene            744..1154
                     /locus_tag="AC16CX_04865"
     CDS             744..1154
                     /locus_tag="AC16CX_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3011880.1"
                     /translation="MGREPVIVLDTHALVWWAAGAAELSTRARRAIQSAARRGSVAAS
                     AISVFEIATAVRRGRLVLAVPVEQWLSDLRLLPELRFEPVSADIAQLAGAFEEAVPHD
                     PADRIIVATALKLQARLVTADDRLRQARRVETIW"
     gene            1475..2800
                     /locus_tag="AC16CX_04870"
     CDS             1475..2800
                     /locus_tag="AC16CX_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011881.1"
                     /translation="MPASKKSAQPESAITPLLPVTIQPGNVRYAPGVRAGRWVFATGH
                     KGTADFAGGMAPEVIDGSAPRHGLPKHKKEAQRIFANFDKVLKAGGTDRDHVVRLDQY
                     YTGAQVVPAYHEVRREFFAGHVPPSTSNLHQKFLLAGQDIEVQLMAAVPAKGFEVAQH
                     RPKNLPVHATSGYSPVLTCGDYVFVAGQTAEALKTEEGPIDPAARMPAGHLWKGTPIK
                     LETEFVIERKLKPALASVGNTLAEVVKAQVYLRDMNDAPAFNEVWAKHFPKAPPATTL
                     IATSTPGFICEAGRIEINTISVRRGGRTRKQVIDAGVPTAYAGHVQAIRAGDLLFLSG
                     LMAVDADGALARAVRTDPGRPYFASAVQLQMDHILAQAQRICRKAGTSLANVARVQQF
                     HTDLADFHGAWQSWDRHLPGRHLSFSAIEVPALAVPGAVVMLDLWVYAP"
     gene            2814..3836
                     /locus_tag="AC16CX_04875"
     CDS             2814..3836
                     /locus_tag="AC16CX_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011882.1"
                     /translation="MKPVSFTLARCRSRFSRALPVLAAAVLAFPAPSPAQTSDAAAGF
                     PPQQIRVVVPFTAGGGTDVIMRLVAKHLADAWKNTVLVDNRVGAGGVVGSQYVTQQKP
                     DGRTFLAVASAFAVRAAIDRSVPYEVTRDFSGVGQMARSPSFMVVSTSHGFGSLKELI
                     AYAKRQPAGIFYTSAGVGSTAHLHAAVVAHLAGIKAEHAPQRGTPEAVNDAMTGRVLY
                     AFAPGPNAIPLARAGKLQILATTSPAGARFLPGIPSLAQAGVQGYEGDDWFGVFAPAG
                     TPLAIRERMSKEMARILALPEIRERLFVLGAEPVSTGPVKFEAFVRDYIARTRKLGEA
                     IGIKPQ"
     gene            3921..4397
                     /locus_tag="AC16CX_04880"
     CDS             3921..4397
                     /locus_tag="AC16CX_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011883.1"
                     /translation="MGSIEARLKQMGYELPPPFVFPKNNRTGCTQAGTLLLLSGHGLD
                     LPKLPNVRQTGKFGADITVEEGYATARAVALTMLATIKAHCGDLDRVKRVLRLFGMCN
                     CTPDFAQQPQVIDGASDLFFELWGPEFGKHARSAVGHAALPRGIAVEINGEFELHA"
     gene            4569..4835
                     /locus_tag="AC16CX_04885"
     CDS             4569..4835
                     /locus_tag="AC16CX_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011884.1"
                     /translation="MPKSLRLGTELEKALDRYCSESGETTSAVIRQSVAEYIVRRQRK
                     RGAPSAWELGRDLFGADRSPAGQGNVSGRVKALIRGKLRAKHHR"
     gene            4816..5217
                     /locus_tag="AC16CX_04890"
     CDS             4816..5217
                     /locus_tag="AC16CX_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3011885.1"
                     /translation="MRSIIVDTGPLVALFHASDRHHEAAKAVLQSNPAVLVTTWPVVA
                     EACHFLGQAGRRALLTFIRRGALRLETLSVEDVPGLDGLLARYAGMDFADATLVLIAE
                     KTGIMEIFTIDRRDFEAYRTPSGRRFRLLLA"
     gene            5494..6480
                     /locus_tag="AC16CX_04895"
     CDS             5494..6480
                     /locus_tag="AC16CX_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011886.1"
                     /translation="MRTSLATCLVLALASVPGAQSAQRPASGGTDASYPARPIRLVVP
                     QAPGGSNDIVARYVGGQLAERVGRQVVVDNRPGAEGTIGTEIVARASPDGYTLLMASA
                     AFTMNPAVVRKLPYDPIKDFDWAAMLGRAPVVITVGPSLPVNSLKELLAVGRSKPNYI
                     TMASAGGFMHFVSAMFRSHAGMDATIALYKGGAPALTDVTGGHAHMAVSTVVTAAPYL
                     RGGKLKPLAIGGTKRVSTLPDVPTVVEAGLPSYDAAIWWGWATAARTPAAVINRLNTE
                     VAAILKLPETVRRFAAEGAEVEIRTPAEIRQMIAADLIKWAKVAQDAGMQKQ"
BASE COUNT         1163 a         2323 c         2201 g         1137 t
ORIGIN      
        1 gcgagtcacg gacgggcttg tacctgctca tgggcaaggc ggtgcggccg atcggccgtc
       61 agaagaacgg cttcgagaaa gcccacaacg cgacgatgga ccgcatggcg cgcgacccgg
      121 cgggctacgc acccggtaat ctcgtcgcct actgcaactc gaaggagttc agggtggatc
      181 tttcggagcc gtgatgggtg atgggggtta cctaaggatg aaggcggaag gatgaaggat
      241 gacccccggc gtacgccgga agtttctcgt cactcattac tcatcaccgt atttaaagcc
      301 ggcccacccg cgagccgcga gtcacgcgtt atcagcaatc ggagataaag ccgatgcggc
      361 gccgaactac tgtctctaca gggtgaatta caggtcatcc ttcagccctc atccccccgg
      421 taggtcctca tccttcatcc ctcgagcctc ggtttgcacc ccaccgattg atcccgatgc
      481 atgttatctg tacactacgt ctgtacacat tggagcaccc cgatgaagga cgtcaaggtc
      541 actgaattga ggcagaacct gccggagtac ctggcgaaga tcaggcgcgg cgagcgcgtg
      601 cgggtgacgt cccggggcaa ggtgatcgcc gagatcgcgc cgccctccgc ctcccggaag
      661 gacgcggaag cggcccgggc gcggctgcgc ggcagcgtgc tcaagtacga gaggccgacc
      721 gaaccggcga tcgatcccgg cgagtgggac gcgaaccggt gatcgtgctc gacacgcacg
      781 cactggtctg gtgggccgcc ggcgcggccg aattgtccac gcgcgcgagg cgggccatcc
      841 agtccgccgc gcgccggggc tcggtggcgg cgtcggcgat cagcgtgttc gagatcgcga
      901 ccgcggtgcg ccggggcagg ctcgtgctcg ccgtccccgt ggagcaatgg ctgagcgact
      961 tgcgtctgct tcccgagctc cggttcgagc ccgtcagcgc cgacatcgcg cagcttgccg
     1021 gcgcattcga ggaagccgtg ccccacgatc ccgcggaccg aatcatcgtc gccacggcgc
     1081 tgaagctgca ggccaggctg gtgaccgcgg atgaccgcct tcgtcaagcg cggcgcgtgg
     1141 aaacaatctg gtaagaaccg ccagccgcca gtcgtcagcc cgccggaacg gcgtgaacgg
     1201 tgaatagtga atggtgaatg gagctgaagc cgggctcctt ctgccttctg ccttagttcc
     1261 tcatccctca tccttgaccc tggaacggcg tgacaagtga cgagtgacga gtgataagtc
     1321 tcccgcgcag cgggctttct taacccccta tcacctatca cctatcactt atcactgggg
     1381 cgggcctccg cccccgcccc atttgtcgtg gccggggttt ggcgcgatac ttcgagcaaa
     1441 gtcgagctga ccgcgtttct tcgagcctga tttgatgccc gccagcaaga agtccgcgca
     1501 acccgaaagc gcaataaccc cattgcttcc ggttaccatc cagcccggca acgttcgcta
     1561 cgcgcccggt gtgcgcgccg gccgctgggt gtttgccacc gggcacaagg gcacggccga
     1621 tttcgcgggc ggcatggcgc ccgaggtgat cgacgggtcc gcgccgcgcc acggcctgcc
     1681 gaagcacaag aaggaggcgc agcgcatctt cgccaatttc gacaaagtcc tgaaggcggg
     1741 cggcaccgat cgcgatcatg tcgtgcggct cgaccagtac tacacgggcg cgcaggtcgt
     1801 tcccgcctac cacgaagtgc ggcgcgaatt tttcgcgggc cacgtgcccc ccagcacctc
     1861 gaacctgcat cagaagttcc tgctcgcggg gcaggatatc gaggtgcagt tgatggccgc
     1921 cgttcccgcc aagggcttcg aagtggcaca gcaccgcccg aagaacctgc cggtgcacgc
     1981 gacctccggc tacagcccgg tgctcacctg cggcgactac gtgttcgtgg ccggccagac
     2041 ggcggaggcg ctcaagaccg aggaaggccc gatcgaccct gcggcacgca tgccggccgg
     2101 gcacctgtgg aagggcacgc cgatcaagct cgaaaccgag ttcgtgatcg agcgcaagct
     2161 caagccagcg ctcgcgagcg tcggaaacac gctcgccgaa gtggtgaagg cacaggttta
     2221 cctgcgcgac atgaacgatg ccccggcgtt caacgaagta tgggcgaagc acttcccgaa
     2281 ggcgccgccc gccaccaccc tcatcgccac gtccacgccc ggattcatct gcgaggccgg
     2341 ccgcatcgag atcaacacga tcagcgtgcg ccgcggcggc cgcacccgga agcaagtgat
     2401 cgacgccggc gtgcccacgg cgtacgccgg tcacgtgcag gcgattcgcg ccggcgacct
     2461 gctgttcctg tccggcctga tggcggtaga cgcggacggc gcgctcgccc gcgcggtgcg
     2521 caccgatccg ggccggccgt acttcgccag cgcggtgcaa ctccagatgg accacatcct
     2581 cgcccaggct caacgcatct gccgcaaggc cggcacgtcg ctcgccaacg tggcgcgcgt
     2641 gcagcagttc catacggatc tcgccgactt ccacggcgcc tggcagtcct gggaccggca
     2701 cttgcccggg cgccacctgt cgttctccgc gatcgaagtg cccgcgctcg ccgtgccggg
     2761 cgcggtcgtg atgctggatc tctgggttta cgctccctga aaggaaggcc atgatgaagc
     2821 ccgtctcgtt tactctcgcc cgttgccgca gccgcttttc gcgggcgctg cctgtcctcg
     2881 ccgccgcagt gctggcgttc cccgcgccgt cgcccgccca gacgtccgac gccgccgcgg
     2941 gcttcccgcc gcagcagatt cgcgtcgtcg tcccgttcac cgcgggcggc ggcacggacg
     3001 tcatcatgcg cctcgtcgcc aagcacctgg ccgatgcgtg gaaaaacacc gtgctggtgg
     3061 acaaccgggt gggcgccggc ggcgtggtcg gctcccagta cgtgacccag cagaagccgg
     3121 acggccgcac cttcctcgcg gtggcgagcg cgttcgccgt gcgcgccgcg atcgaccgca
     3181 gcgtgcccta cgaagtcacc cgggatttct ccggcgtcgg gcagatggcg cgctcgccga
     3241 gcttcatggt cgtgtccacg tcgcacggtt tcggctcgct caaggagctg atcgcgtacg
     3301 cgaagaggca gcccgccggc attttctaca cctcggccgg cgtcggcagc accgcccacc
     3361 tgcatgcggc cgtggtggcg cacctcgccg gtatcaaggc cgagcatgcg ccgcagcgcg
     3421 gcacgccaga agcggtcaac gacgcgatga cggggcgcgt gctctacgcg ttcgcgccgg
     3481 ggccgaacgc gatcccgctc gcgagggcgg gcaagctgca gatcctggcg acgacctcac
     3541 cggccggcgc gcgctttctg ccgggcatac ccagcctcgc gcaggccggg gtgcaaggtt
     3601 acgaaggcga cgactggttc ggcgtgttcg cgccggccgg cacgccgctc gcgatccgcg
     3661 agcgcatgtc gaaggaaatg gcgcgcatcc tcgcgctgcc cgaaatccgc gagcgcctgt
     3721 tcgtgctcgg cgccgagccg gtttccaccg gcccggtgaa attcgaggct ttcgtgcgcg
     3781 attacatcgc ccgcacacgc aagctcggcg aggccatcgg catcaagccc cagtgaccgt
     3841 gataggtgat aggtgatagg tgatgggtga tgagtgatgg gttatgggac tcgaccccga
     3901 gtcaaacagg gagcgcacgc atgggatcga tcgaagcacg cctgaagcaa atgggctacg
     3961 agctgccgcc gccgttcgta ttcccgaaaa acaaccgcac cggctgcacc caggccggca
     4021 ccctgctcct gctgtcggga cacgggctcg acctgccgaa gctgcccaac gtccggcaga
     4081 ccggcaagtt cggcgcggac atcacggtgg aggaaggcta cgcgaccgcg cgcgcggtcg
     4141 cgctcaccat gctcgccacg atcaaggcgc actgcggcga tctcgaccgg gtgaagcgcg
     4201 tgctgcgcct gttcggcatg tgcaactgca cgcccgattt cgcccagcag ccgcaggtca
     4261 tcgacggcgc ctcggatctc tttttcgagc tctggggacc cgagttcggg aagcacgccc
     4321 gctcggcggt ggggcacgcg gcgcttccgc gcggcatcgc ggtggaaatc aacggcgagt
     4381 tcgagttgca tgcgtagcag gccagtgatc cgggagtcgt gattcgactc agcactcagc
     4441 actgagaatt caatcccggg gttttcccct tcctcctcac tcctcacccg ccacgagggg
     4501 ggctttgcac ccggtggcgc tggccagtgt gatacaattc aaccattgtg tatcacgtgg
     4561 agttggccat gcccaaatcg ctccgattgg gaaccgaatt ggagaaagcg ctcgaccgct
     4621 attgctctga aagcggggaa acgacgagcg ccgtcatccg ccagagcgtg gcggaataca
     4681 tcgtccggcg gcagcgcaag cgcggcgccc cgtccgcgtg ggaactgggc cgggacttgt
     4741 tcggcgccga ccgctcgccg gccgggcaag gcaacgtctc cgggcgcgtc aaggcgttga
     4801 tccgcggaaa gctccgtgcg aagcatcatc gttgacacag ggccgcttgt cgcgctgttc
     4861 cacgccagcg accggcacca cgaggccgcc aaggccgtcc tgcagtcaaa cccggcagtg
     4921 ctggtgacca cctggcctgt agtcgcggaa gcctgccact tcctcggcca ggccggaagg
     4981 cgcgccctgc tcactttcat ccgccgcggc gcgttacggc ttgaaacctt gtccgttgaa
     5041 gacgtgcccg gcctcgacgg cctgctcgcg cgctacgccg gcatggattt cgccgacgcg
     5101 acgctggtgc tcatcgccga aaagaccggg atcatggaga tcttcacgat cgaccggcgg
     5161 gattttgagg cctatcggac gccttccggc cggcgcttca ggctcctgct ggcgtagccg
     5221 tacggcggga acggcgtgac cggtgacctg tgaccggtag ttgagcctcc cgcgcagcgg
     5281 gcttacgaaa actcctatca cctatcacct atcacttatc actggggcga gcctccgccc
     5341 ccgtcccatt tgtcatggcc gaggtttggc gcgatacttt tccacaaaaa tcgactctga
     5401 cctcatttac tttgcattta cttttcgata cttccgcggc agcacgtatg gcgctgccgt
     5461 ctcgcaggct gacaagcgac taagggggag ccaatgagaa caagccttgc aacgtgcctg
     5521 gtcctcgcgc tcgcatccgt acccggcgcg cagtcggcac agcggccggc ctcgggcgga
     5581 acggatgcga gctatcccgc caggccgatc cgcctcgtcg tgccgcaggc gcccggcggc
     5641 agcaacgaca tcgtagcccg ctacgttggg ggccagctgg ccgagcgcgt cggccgccag
     5701 gtggtggtcg acaaccgtcc tggcgcggaa ggcacgatcg gcaccgagat cgtcgcgcgc
     5761 gcttcccccg acggctacac gctgctcatg gcctcggccg cgttcacgat gaatccggcg
     5821 gtggtgcgca agctgcccta cgatccgatc aaggacttcg actgggccgc gatgctcggc
     5881 cgcgcgccgg tcgtgatcac cgtggggccc tcgctgccgg tcaactcgct gaaggagctg
     5941 cttgccgttg gaaggtccaa gccgaactac atcacgatgg cgtcggcggg cggcttcatg
     6001 catttcgtgt cggcgatgtt ccgcagccac gccgggatgg acgccaccat cgcgctctac
     6061 aaaggcggcg cgccggcatt gaccgacgtg acgggcggcc acgcgcatat ggcggtgtcc
     6121 actgtcgtaa ccgcggctcc gtatcttcgc ggcggcaagc tcaagccgct cgcgatcggc
     6181 ggcacgaagc gcgtctcgac cctgcctgat gttccgacgg tcgtcgaggc cgggctgccg
     6241 agctatgacg cggcgatctg gtgggggtgg gcgacggcgg cacgcacgcc ggccgccgtc
     6301 atcaaccggc tgaacaccga agtcgcggcc atcctcaagc tcccggagac cgtgcgccgc
     6361 ttcgccgcgg agggtgccga ggtcgaaatc cggacccccg ccgaaatccg gcagatgatc
     6421 gccgccgacc tcatcaagtg ggccaaggtc gcgcaggacg ccggcatgca aaagcagtga
     6481 cgagctgcgg gcgacagcaa atacaccgaa ggatgaggga tgaaggatga gggatgaccc
     6541 ccgcagtgcg ggcgacccat cactcatcgc ccatcaccgg gtttcgggtc atccttcatc
     6601 cttccgcctt catccttcgg gatacccctc atccctcatc cctgaccctg gaacggcgtg
     6661 acgagtgacg cgtgacgagt gataagtctc ccgcgcagcg ggctttctta accccctatc
     6721 acctatcacc tatcacttat caccagcgcc acgggggcag gcctgtctgc ccgtccttgg
     6781 tggcgctcgt tggggtttgg cgcgatactc tcccggcatg tttt
//
LOCUS       DBNGWZ010000165        12724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1401711, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000165 DBNGWZ010000000
VERSION     DBNGWZ010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1401711"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /gene="smpB"
                     /locus_tag="AC16CX_04900"
     CDS             <1..293
                     /gene="smpB"
                     /locus_tag="AC16CX_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR3011887.1"
                     /translation="FLTGCHVSPLPAASTHFIPDPTRTRKLLLHAAEISRLIGSVERA
                     GYAVVPLDLHYKGGRIKLEIGLAKGKKQYDKRAAEKEKDWRREQQRLLRVKH"
     gene            complement(332..871)
                     /locus_tag="AC16CX_04905"
     CDS             complement(332..871)
                     /locus_tag="AC16CX_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011888.1"
                     /translation="MQFLWWHWVVLGIVLTLLELAVPSFFLVWFGLGALIVGITLLAR
                     LHLAMVQGVQAIQDIKPSESMVKSMEMQAAERERKAVVTRAEGEKQAMILEAEGRLES
                     AKRDVVAQVTLAESASQAIQKVAHAVGDRDTPMLFLLGEKYIQALDKMSASQNSKIVL
                     LPGDLQDALRGVLGKARSA"
     gene            complement(1026..1100)
                     /locus_tag="AC16CX_04910"
     tRNA            complement(1026..1100)
                     /locus_tag="AC16CX_04910"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1066..1068),aa:His,seq:gtg)
     gene            complement(1216..1291)
                     /locus_tag="AC16CX_04915"
     tRNA            complement(1216..1291)
                     /locus_tag="AC16CX_04915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1256..1258),aa:Arg,seq:tct)
     gene            1496..1918
                     /gene="dksA"
                     /locus_tag="AC16CX_04920"
     CDS             1496..1918
                     /gene="dksA"
                     /locus_tag="AC16CX_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR3011889.1"
                     /translation="MATELHKPYPPYKPKGKEDYMNPRQLAHFRKMLEEMKRELSQDI
                     DRTVHTMQDEATIFADPNDRASQESDMSLELRNRDRERKLIKKIDETIARIDAGEYGY
                     CDSCGVEIGLKRLEARPTATLCIDCKTLDELRERQVAK"
     gene            complement(2037..4148)
                     /gene="pnp"
                     /locus_tag="AC16CX_04925"
     CDS             complement(2037..4148)
                     /gene="pnp"
                     /locus_tag="AC16CX_04925"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3011890.1"
                     /translation="MNHVKKSLVWGRHRLILETGEIARQATGAVLVNMEDTVVLVTVV
                     GAKQAQPEKDFLPLTVDYQERTYAAGKIPGGFFKREGRPSEKEILTCRLIDRPIRPLF
                     PDGFYNEVQLVATVVSSNNEIDSDIPAMIGASAALALSGIPFNGPIGASRVGYLNGQY
                     VLNPTATELKTSQLDLVVAGTEQAVLMVESEARELSEDVMLGAVVFGHQQMQQVIQMI
                     NELVDEAGSEVWDWAPPAKDETLIARIAELAENDLREAFQIKQKQGRSEAIDNIWKRV
                     FTEVGVGTEGGPEANAVKEICFALESKIVRSQILNGDPRIDGRDTRTVRPISIRTGLL
                     PRAHGSALFTRGETQALVAATLGTSRDEQRIDALQGEYTDRFMMHYNMPPYATGETGR
                     VGSPKRREIGHGRLAKRALLAMLPAPEEFSYSLRVVSEITESNGSSSMASVCGGCLAL
                     MDAGVPLKTHVAGIAMGLIKEGNRFAVLSDILGDEDHLGDMDFKVAGTENGVTALQMD
                     IKINGITKEIMHAALVQARDGRMHILEKMRQALEAPRSEISVWAPRIITIKIKPEKIR
                     DVIGKGGAVIRALTEETGTTIDIQDDGTVTIACVSPEAGEAARKRIEDITADVEVGRV
                     YDGTVLKLLDFGAIVSVLPGKDGLLHISQIATERVNNVSDHLKEGQHVRVKVLEADEK
                     GRLRLSMKAVTLEERAATTAQ"
     gene            complement(4291..4560)
                     /gene="rpsO"
                     /locus_tag="AC16CX_04930"
     CDS             complement(4291..4560)
                     /gene="rpsO"
                     /locus_tag="AC16CX_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3011891.1"
                     /translation="MAVTNTQKAQLVKEYQRATGDTGSPEVQIALLTARITDLTEHFK
                     THVKDHHSRRGLLRMVSRRRKLLDYLKRVDADKYRQLIDRLGLRK"
     gene            complement(4592..5557)
                     /gene="truB"
                     /locus_tag="AC16CX_04935"
     CDS             complement(4592..5557)
                     /gene="truB"
                     /locus_tag="AC16CX_04935"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3011892.1"
                     /translation="MNSARAQWRAVDGVLLLDKPAGITSHTAVQKVRRLFNAAKAGHT
                     GTLDPLATGLLPVCLGEATKFSHLLLEADKNYLASIRLGVSTTTGDLEGEVTTRLEVD
                     MNQEKIESVLKNFVGEILQTPPMYSAIKQGGQPLYKLARAGRELPRAPRKVSINSLTL
                     VDLAGDELTVRVCCSKGTYVRVLAEDVGRELACGACLSALRREAVGAFTLCFGAVTLQ
                     QLELLTMAEREALLMPADALVAALPRFDLDAVETRRLSRGQPVERPEAHAVAGLVRIY
                     GPDHEFLGVAEVTRPGHIVPRRLKSQARATGSTGQVLLEKPGVRE"
     gene            complement(5554..5931)
                     /gene="rbfA"
                     /locus_tag="AC16CX_04940"
     CDS             complement(5554..5931)
                     /gene="rbfA"
                     /locus_tag="AC16CX_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3011893.1"
                     /translation="MPKDYPRSRRIAEQVQRELSDIIRLELKDPRVGMITITDVEVSL
                     DQSNAKVFFTLLGEPSRIADATEGLRHAAGFLRSQLAHRMKLRIVPQLQFKYDESVER
                     GMRLSQLIDAAVAQDAARTRKRK"
     gene            complement(6015..8546)
                     /gene="infB"
                     /locus_tag="AC16CX_04945"
     CDS             complement(6015..8546)
                     /gene="infB"
                     /locus_tag="AC16CX_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3011894.1"
                     /translation="MAQINVTQFAQQLGLPTALLIEQLQAAGVNKALAEDTPLTEKDK
                     TQLLEHLRKSHGAAEAKQKITLTRRQTTEIKKSDGAGKSRTIQIEVRKKRVLVKREAA
                     EPDATAEAVRPVLDAEEIAQREEEARKQAELAARQAEDAEKKRRKKQEAAEVVAELKA
                     TEEARPAAVQAAAISVEGTLHKPTVKPEEKAEKAEKKAKKQVKTVVWKDETVKRRTIK
                     TRGDASGGLGWRERKARHAAHKEEGDQQRAFAAPTEPIVREVTVPETITVADLAHKMS
                     VKAAEVIKVLMKLGTMATINQVLDQDTAMIVIEEIGHVAKRAKLDEPDAFLVEQQQEA
                     PAEIKLEPRAPVVTVMGHVDHGKTSLLDAIRRTRVASGEAGGITQHIGAYHVNTPKGM
                     VTFLDTPGHEAFTAMRARGAKITDIVVLVVAADDGVMPQTVEAIHHAKAAKVPIVVAL
                     NKIDKPEANPDRVKQDLSSQEVVPEEWGGDTMFVEVSAKTGKGIDSLLERVLLQAEVL
                     ELKAPGDAPAKGVVIESKLDKGRGPVATVLVQSGTLKRGDVVLAGAVFGRVRAMLDET
                     GGAIESAGPSLPVEILGLSDVPAAGSEVLVLGDERKAREIALFRQGKFRDVKLAKQQS
                     IKLESIFEQMGEGQAKTLSLIIKADVQGSYEGLSHALSRLSTDEVKVNIVHAAVGGIT
                     ESDVNLALASKAVIIGFNARADATARKLAEHNGINIRYYNVIYDAVDDVKAALSGMLA
                     PERKENVLGLVEVRQIFKISKVGTVAGCYVSEGLVRRSNAKVRVLRDNVVVHDGELDS
                     LKRFKDDVREVKAGFECGLSLRNFDDIKVGDQLEVYEVVEVARTL"
     gene            complement(8559..10043)
                     /gene="nusA"
                     /locus_tag="AC16CX_04950"
     CDS             complement(8559..10043)
                     /gene="nusA"
                     /locus_tag="AC16CX_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3011895.1"
                     /translation="MSREILLLVDALGREKNVDKEIVFGALELALASATKKRFHEDID
                     VRASVDRATGGFSFFRRWQVMNEGEVEEPSRQIVLSDAKKENPDIQPGDYIEKPLGAL
                     EVGRIGAQTAKQVILQKIRDAEREQILNDFLSRKEHLVTGVIKRMERGNAIIESGRLE
                     AVLPRDQMIPKENLRVGDRVRAYVWKTDRASRGPQLVLSRTAPEFIVKLFELEVPELE
                     DGLLEIKAAARDPGSRAKIAVHSKDRRIDPIGTCVGMRGSRVQAVTSELAGERVDIVL
                     WAEDPAQFVINALAPAEVSKIRVDEEAHGMDIVVDEDNLAQAIGRNGQNVRLASEMTG
                     WELNIMTMEESQKKSQEEATRVRTMFMERLDVDEEVADILVQEGFNTLEEVAYVPISE
                     MLEIESFDEATVNELRSRARNALLTQAIVSEEQVGHDIEDLMKVEGMDNETARLLAAK
                     GVGTQEALADFATDDLVELTGLDNERAKQLIMAARAPWFAEANS"
     gene            complement(10056..10484)
                     /gene="rimP"
                     /locus_tag="AC16CX_04955"
     CDS             complement(10056..10484)
                     /gene="rimP"
                     /locus_tag="AC16CX_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR3011896.1"
                     /translation="MDLNGLLETTLGGMGYELVGVERPGRGRLLRIYIDKPGGVTVDD
                     CAAVSHQVSRVLAVENVNYDRLEVSSPGLDRLLKGERDFVRFAGQRARVRTRVPVDGQ
                     RNFVGVVREAGAGRLQLEVDGRLLSLEIGNVETARLVPNL"
     gene            10635..11864
                     /locus_tag="AC16CX_04960"
     CDS             10635..11864
                     /locus_tag="AC16CX_04960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR3011897.1"
                     /translation="MPLPLRALYVDFNSYFASVEQQLRPELRGKPVAVLPVIADTTCC
                     IAASYEARKFGIKTGTLVREARRLCPGLQLVEAQPATYVEYHHRLVAAVESRTHVEKV
                     LSIDEMYCELLGHERRRDRALALAQEIKHAIAAKVGAELRCSIGIAPNVFLAKTASNL
                     QKPDGLTVIEQRNLPDCLFGFGLRDLCGIGRNMEKRLNALGIASVEALCKAGKPVLRR
                     AWGGVGGERMYAKLRGEWVDDLPTERSSIGHSHVLPPALRSPHAALSVLHRLLQKAAM
                     RLRSYGLIAAALHVHVGRLDGSSWENLIRIDPTQDTLRLLDALKALWAGYPAGSKAAP
                     LKVGVTLLDLHEQSGQTRSLFDDLEIRSRLNAAVDRLNLRYGKNTLYFGGAYLALRSA
                     PMRIAFNHIPDPMVEGD"
     gene            complement(12046..12160)
                     /gene="rrf"
                     /locus_tag="AC16CX_04965"
     rRNA            complement(12046..12160)
                     /gene="rrf"
                     /locus_tag="AC16CX_04965"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(12314..>12724)
                     /locus_tag="AC16CX_04970"
     rRNA            complement(12314..>12724)
                     /locus_tag="AC16CX_04970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2260 a         4233 c         3719 g         2512 t
ORIGIN      
        1 ttttcctcac cggctgccac gtgagcccgt tgcctgccgc ttctacgcac ttcattcccg
       61 acccgacgcg cacgcgcaag ctcctgctgc acgccgctga gatcagccgg ctcatcggca
      121 gcgtcgagcg cgccggctac gccgtggtac cgctcgatct gcattacaag ggcggccgca
      181 tcaagctcga gatcgggctg gccaagggaa aaaagcagta cgacaagcgc gcggccgaaa
      241 aggaaaaaga ttggcgccgc gagcaacaac gcttactgcg ggtcaagcac tagcgctgct
      301 ggtaccagtc ttcggtcact ggccactgtt ttcaggccga tcgcgccttg ccgagtaccc
      361 cccgcagcgc atcctgcagg tcgccgggca ggagcacgat cttgctgttt tgagaggcgg
      421 acatcttgtc gagcgcctgg atgtacttct ccccgagcag gaacagcatc ggcgtgtcgc
      481 ggtcgccgac cgcgtgcgcg actttctgaa tcgcctgcga cgccgactcg gcgagcgtca
      541 cctgcgccac cacgtcgcgt tttgcgctct ccagcctgcc ttcggcctcc aggatcatgg
      601 cctgcttctc gccctcggcc ctggtcacga cggcctttct ctctcgttcc gccgcctgca
      661 tctccatcga cttcaccatg gactccgaag gcttgatgtc ctggatcgcc tgaacacctt
      721 gaaccatagc cagatgaagg cgagcgagca gcgtgatgcc gacgatcaac gcccccagcc
      781 cgaaccacac caggaagaac gacggcacag cgagctcgag cagcgtcagc acaatgccga
      841 gcaccaccca atgccaccac aggaattgca ttgcgcctcc ctgccgggaa caccgcaacg
      901 attagagcat gattagcgcg cccgggcaaa ggcgcggttt caagcgtcga tcttgagtgc
      961 cgagcaattg gataatattc ggtttatcgc tcgacacctg acactcgccc gaacgggcga
     1021 aaggatgggg tggctgatgg gactcgaacc cacaacacct ggaatcacaa tccagtactc
     1081 taccgttgag ctacagccac cgtcagcaaa accagtatcg agtgctgagt tctgagtcct
     1141 gagttagacc ggccacgttc ggtggttatc cagcactatg cactcagcac ttggcactgc
     1201 tcgcaggggc gaatctggcg cgcccggcgg gaatcgaacc cacaaccccc ggcttagaag
     1261 gccggtgctc tgtccgttga gctacgggcg cgtaccggca acttcgccgc gtccggtcac
     1321 gggtcgcaaa gcgaggcggc aaaccgcgcg gggcggcgcc ttggacgcgg cgtaatatac
     1381 ccttggaagc ttctgatcgt caatgaaatt tcggcatcgt tccagcgcaa aaaatcagct
     1441 tgcagcgcct gtcgatttca gctataaagc gtcttttttc gagcgccaca ggcacatggc
     1501 caccgaattg cacaagcctt atccccccta caaacccaag gggaaagagg actacatgaa
     1561 cccgcgccag ctcgcccatt tccgcaagat gctggaggag atgaagcgcg agctgtccca
     1621 ggacatcgac cgcacggtgc acaccatgca ggacgaggcg acgatcttcg ccgatcccaa
     1681 cgaccgcgcc agccaggaat cggacatgtc gctggagctg agaaaccgcg accgagagcg
     1741 caagctcatc aagaagatcg acgagaccat tgcgcgcatc gacgccggcg aatacggcta
     1801 ttgcgattcc tgcggggtgg aaatcggcct gaagcggctc gaggcgcgtc cgaccgcgac
     1861 gctctgcatc gactgcaaga cgctcgatga actgcgcgag cggcaggtcg ccaagtagca
     1921 atcagcattc ggctgtcagc agtcagcgaa ttgcggggcg gaagcaataa aggggcggaa
     1981 tcaccgcccc ttcttgcttg gatgaatact gacggctgac tgctggcagc tgacggctat
     2041 tgggcggtcg ttgccgcccg ctcttccaag gtgaccgcct tcatggacag ccgcaggcgt
     2101 cccttttcgt cggcttccag caccttgacg cgcacgtgct gtccttcctt gagatggtcc
     2161 gagacattat tgacgcgctc ggtggcgatc tgggagatgt gcagcaggcc gtcctttccg
     2221 ggaagcacgc tcacgatcgc gccgaaatcg agcagtttca acactgtgcc gtcataaacg
     2281 cggccgactt ccacatccgc ggtaatgtcc tcgatgcgct tgcgcgccgc ctcgcccgct
     2341 tccggcgaca cgcacgcgat ggtgacggta ccgtcgtcct gaatgtcgat cgtggtgccg
     2401 gtctcctccg taagtgcccg gatcaccgcg ccgcccttgc cgatgacgtc gcggatcttc
     2461 tccggcttga tcttgatcgt gatgatgcgc ggcgcccaca cggaaatctc cgagcgcggc
     2521 gcttccagcg cctgcctcat cttctccagg atgtgcatgc ggccatcacg cgcctggacc
     2581 agcgcggcgt gcatgatctc cttggtgatg ccgttgatct tgatgtccat ctggagcgca
     2641 gtgacgccgt tctcggtgcc ggcgaccttg aaatccatgt cgcccaggtg gtcctcgtcg
     2701 ccgaggatgt cggacagcac cgcgaaccgg tttccttcct tgatgagccc catggcgatg
     2761 cccgccacat gtgtcttcaa gggcacgccg gcgtccatca gcgcgaggca gccgccgcac
     2821 acgctcgcca tcgagctcga accgttggac tcggtgatct cggaaaccac acgcagcgag
     2881 tagctgaact cctcgggagc cggcagcatg gcgagcagcg cgcgcttggc gagccgcccg
     2941 tgcccgattt cgcggcgctt gggcgagccg acacgtccgg tctcgccggt cgcgtagggc
     3001 ggcatgttgt aatgcatcat gaagcgatcc gtgtactcgc cctgcaacgc atcgatgcgc
     3061 tgctcatcgc gcgaggtgcc gagagtagcg gcgaccagcg cctgggtttc gccgcgggtg
     3121 aacaaagcgg agccgtgcgc gcgcggcaga agaccggtgc gtatcgagat cgggcgcacg
     3181 gtgcgcgtgt cgcggccgtc gatacgcggg tcgccattga ggatctggct gcgcacgatt
     3241 ttcgattcca gcgcgaagca gatttccttg accgcattgg cttccggacc gccttccgtg
     3301 ccgactccaa cttcggtgaa cacacgcttc cagatattgt cgatcgcctc ggaacggccc
     3361 tgcttctgct tgatctggaa cgcctcacgc aaatcgttct cggccagttc cgctatgcgc
     3421 gcaatcaacg tctcgtcctt ggcgggcggc gcccaatccc acacctccga gcccgcctca
     3481 tccaccagct cgttgatcat ctgtatcact tgttgcatct gctggtgtcc aaataccacc
     3541 gcgccgagca tcacgtcctc ggacagctcc ctcgcctcgg attccaccat cagcaccgcc
     3601 tgctcggtgc cggccaccac gaggtcgagc tgcgaagtct tgagctcggt ggcggtcggg
     3661 ttgagcacgt actgcccgtt gagatagccg acacgggacg ccccgatcgg gccattgaac
     3721 ggaatgccgg aaagggcgag cgcggccgat gccccgatca tcgccggaat gtccgagtcg
     3781 atctcgttat tcgacgaaac cacggtcgca accagctgca cttcgttata aaagccgtcc
     3841 ggaaacagcg gccggatcgg gcggtcgatc agccggcagg tgaggatctc cttctccgac
     3901 ggccggcctt cgcgcttgaa aaaaccgcct ggaatcttgc cggcggcata agtgcgttcc
     3961 tggtaatcca cggtaagcgg caggaaatcc ttctccggct gcgcctgctt ggcgccgacc
     4021 acggtgacca gcaccacggt gtcttccata ttgaccagca ccgcgcccgt cgcctggcgc
     4081 gcgatttccc cggtctcaag gatcagcctg tgccgacccc ataccagcga tttcttgacg
     4141 tgattcaatg tcctgcccct tgaaaaataa aaaagaccgc cgtgcgcggt ctacggttaa
     4201 ttataggtcg cggaacgccg tccctgcggt cccatgggac ccggtgtgac tcccaaccgg
     4261 tcgctcgcga cgccgcccat ggcgggtgct ttatttacgc agacccaggc ggtcaatcag
     4321 ctgacggtac ttgtcggcgt ccactctctt gaggtagtcg aggagctttc gcctgcggct
     4381 caccatccgc agcaggccgc gccgcgaatg atggtccttg acgtgcgtct tgaagtgctc
     4441 ggtgaggtcc gtgatgcgcg ccgtcagcaa cgcgatctgg acctcgggtg atccagtgtc
     4501 gcccgtggca cgctggtatt ccttgaccag ctgggctttc tgggtgttgg taaccgccat
     4561 cattttctcc gcctgtcagt cgcgcgcaat tttactctct aacccccggt ttctcaagca
     4621 atacttggcc agttgatccg gtggcccgcg cctgggattt gagccgccgt ggcacgatat
     4681 gccccggcct cgtgacctcg gctacgccca agaattcgtg atcaggacca tagatacgca
     4741 cgagccctgc caccgcatgg gcctcggggc gctcgaccgg ctgaccccgg ctcaaacgtc
     4801 gcgtttccac ggcatcgaga tcgaagcgcg gcagcgcagc taccagcgca tcggccggca
     4861 tcaacaaagc ctccctctca gccatcgtca atagctcgag ctgttgcagg gtgaccgctc
     4921 cgaaacaaag cgtaaaggcg ccgaccgcct cgcgccgcag cgcggacagg caggccccgc
     4981 aagccagctc gcgtccaacg tcttcggcca gtacgcgcac atacgtgccc ttgctgcaac
     5041 aaacccgcac ggtcaattca tccccggcaa gatcgaccag ggtcaggctg ttgatggata
     5101 ccttgcgcgg cgcacgcggg agctcacgtc ctgctcgcgc gagcttgtag agcggctgcc
     5161 caccctgttt gatggcgctg tacattggtg gcgtctgaag aatttcgccc acgaaattct
     5221 ttaggacaga ctcgattttc tcttgattca tatcgacttc gagccgcgtt gtgacctcgc
     5281 cttcgaggtc accagtggtg gtggacacgc ccagccggat cgaggcgaga tagttcttgt
     5341 ctgcctccag cagcaagtgg gaaaacttgg tggcctcgcc cagacagacc ggcagcaggc
     5401 ctgtagcaag cgggtcgagc gtcccggtgt ggccggcttt ggccgcgttg aacaggcgtc
     5461 gtaccttctg caccgcagta tgcgaagtga tgccggccgg cttgtccagc agcaacacgc
     5521 cgtccacggc gcgccattgc gcccgcgcgc tgttcatttt ctcttccggg tccgcgccgc
     5581 gtcctgggcg acggccgcgt cgatcagctg cgacaggcgc atcccgcgct cgaccgattc
     5641 gtcgtacttg aactgcagct gcggcacaat gcgcagcttc attcggtgcg cgagctggct
     5701 gcgcaggaat cccgcggcgt gccgcaggcc ctcggtggcg tcggcgattc tggatggctc
     5761 gcccagcagc gtgaaaaata cctttgcatt ggactggtcc aggctgactt cgacgtcggt
     5821 aatggtgatc atgccgacac gcgggtcttt cagctcgagc cggatgatgt ccgacagctc
     5881 gcgctggact tgctcggcga tgcggcggct gcggggatag tctttgggca tagctgcgta
     5941 aggcgtgagg ggttaggcgt aaggcgatca acccgtggtt cccgtctcac tcctcactcc
     6001 tcacgcctaa cgcttcacag ggtccgggcg acctcgacga cttcgtacac ctcgagctgg
     6061 tccccgacct tgatgtcgtc gaagttcctg agggaaaggc cgcactcgaa tccggccttg
     6121 acttcccgca cgtcgtcctt gaaacgcttg agcgaatcca gttcgccatc gtgtacgacc
     6181 acgttgtcgc gcagcacgcg caccttggca ttgctgcgcc gtaccaaccc ctccgacaca
     6241 tagcagccgg cgacggtgcc gaccttggaa atcttgaaaa tctggcgcac ctcgacgaga
     6301 ccgaggacgt tctccttccg ctccggcgca agcatgccag aaagcgccgc cttgacgtcg
     6361 tcaacggcat cgtagatcac gttgtagtag cggatgttga tgccgttatg ctcggcaagc
     6421 ttgcgcgccg tggcgtccgc ccgcgcgttg aacccgatga tgaccgcctt ggaagccagc
     6481 gcgagattga cgtccgactc ggtgatgccg cccacggcgg cgtgcacgat attgaccttg
     6541 acctcatcgg tagagagcct ggacagggcg tgcgacaggc cctcgtaaga accctgtaca
     6601 tccgccttga tgatgagcga gagcgtcttg gcctgcccct cgcccatctg ctcgaagatg
     6661 ctttcgagct tgatcgactg ctgtttcgcc agcttgacgt cgcggaactt gccctggcgg
     6721 aacaatgcga tctcgcgtgc cttgcgctcg tcgcccagca ctagcacctc ggaacccgcc
     6781 gccggcacgt cggaaagacc gaggatttcc accgggagcg aaggaccggc cgattcgatc
     6841 gcgccgccgg tttcgtccag catggcccgc acgcgcccga acaccgcccc ggccagcacg
     6901 acgtcgcctc gcttgagcgt tcccgactgc accagaacgg tagcgacggg gccgcgcccc
     6961 ttgtcaagtt tggattcaat gacgacgccc ttggccggcg cgtccccggg cgccttcagt
     7021 tcgagcactt cagcctgcaa caggacccgc tccagcagcg aatcaatgcc cttgccggtc
     7081 ttggccgaga cctcgacgaa catcgtgtcg ccgccccact cttccgggac gacctcctgc
     7141 gacgacaggt cctgtttcac gcgatccgga ttggcttccg gcttgtcgat cttgttgagc
     7201 gccaccacga tcggcacttt tgccgccttg gcatggtgga tggcttctac ggtttgcggc
     7261 attacgccgt catcggcggc gacgaccagc accacgatat cggtgatctt ggcgccgcgt
     7321 gcccgcatcg ccgtgaacgc ctcgtggccg ggcgtgtcga gaaaggtgac catgcccttg
     7381 ggcgtattca catgataggc gccgatgtgc tgggtaatgc caccggcctc cccgcttgcc
     7441 acccgggtgc ggcgtatagc gtcaagcaac gaggtcttgc cgtggtccac atgacccatc
     7501 acggtcacca ccggcgcgcg cggctccagc ttgatttcag cgggggcttc ctgctgctgc
     7561 tcgacgagga acgcgtcggg ctcgtcgagc ttggcgcgct tggcgacgtg gcctatttcc
     7621 tcgatcacga tcatcgccgt gtcctggtcg agcacctggt tgatggtcgc cattgtgccg
     7681 agcttcatca gcaccttgat cacctcggcg gccttgaccg acatcttgtg cgccagatcg
     7741 gcgacggtga tggtttccgg caccgttact tcccgcacga tgggctcggt tggcgccgcg
     7801 aatgcgcgct gctgatcgcc ttcctccttg tgggcggcgt ggcgtgcctt gcgttcgcgc
     7861 catcccagcc cgcctgaagc atcaccgcgg gtcttgatcg tgcgccgttt gacggtttcg
     7921 tccttccaga cgaccgtttt gacctgcttc tttgcctttt tctcggcttt ttcggctttt
     7981 tcctccggct tcaccgtggg cttgtgaagc gtgccctcga ccgagatcgc cgccgcctgc
     8041 acggccgcgg gtcgggcttc ctccgtcgcc ttgagttcgg cgacgacttc agccgcctcc
     8101 tgcttcttgc gccgcttttt ctctgcgtcc tccgcctgcc gtgccgccaa ttcagcctgt
     8161 ttcctggctt cttcctcgcg ctgcgcgatc tcttccgcat cgagcacggg cctcactgcc
     8221 tccgccgtcg cgtcgggctc agcggcctcg cgcttcacga gcacgcgctt cttgcgcacc
     8281 tcaatctgga tggtgcgcga cttgccggcg ccgtcggact tcttgatttc ggtggtctgc
     8341 cgccgcgtga gcgtgatctt ctgcttcgcc tcggcggcgc cgtgcgactt gcgcaggtgc
     8401 tcaagcagct gggtcttgtc tttctcggtg agcggcgtgt cttcggccag ggccttgttc
     8461 acgccagcgg cctgcagctg ctcgatgagc agtgcggtcg gcagccccag ctgctgggcg
     8521 aattgcgtga cgttgatctg cgccatcagg tgcgggcctc aggagttggc ttcggcaaac
     8581 cagggggctc gcgcggccat aatgagctgc ttggcgcgct cgttatccag tcccgtcagc
     8641 tcgaccaggt cgtcggttgc gaaatcggcc agcgcctcct gcgtgcccac ccctttggcg
     8701 gcaagcaggc gcgcggtctc gttgtccatg ccttcgacct tcatcaggtc ctcgatgtcg
     8761 tgcccgacct gctcctcgct cacgatcgcc tgggtgagca gcgcgttgcg ggcgcggctc
     8821 ctcagctcat tcacggtggc ttcgtcgaag gactcgattt ccagcatttc gctgatcgga
     8881 acgtaggcga cctcctccag ggtattaaag ccctcttgga ccaggatgtc ggcgacctcc
     8941 tcgtcgacgt cgagcctttc catgaacatc gtgcgcacgc gcgtcgcttc ctcctggctt
     9001 ttcttctgcg attcctccat cgtcatgatg ttgagttccc agccggtcat ctcgctcgcc
     9061 aatcgcacgt tctggccgtt gcgcccgatc gcctgggcga ggttgtcctc gtccaccacg
     9121 atgtccatgc cgtgcgcttc ctcgtccacc cggatcttgc tcacctcggc cggggccagc
     9181 gcattgatca cgaactgggc gggatcctcg gcccacagca cgatgtccac gcgctcgccg
     9241 gcgagctccg aagtcaccgc ctggacgcgc gatccgcgca tgccgacgca ggtgccgatc
     9301 ggatcgatcc ggcggtcctt ggagtgaacc gcgatcttcg cccgcgaacc ggggtcgcgg
     9361 gcagccgcct tgatctcgag cagcccgtcc tcgagctcgg gcacctcgag ctcgaacagt
     9421 ttgacgatga actcgggggc cgtgcgcgaa agcacgagtt gggggccccg gctcgcgcgg
     9481 tctgtcttcc acacgtaggc ccgcacccgg tcacccacgc gcaggttctc cttcgggatc
     9541 atctggtcgc gcggcagcac ggcttccagc cgccccgact cgatgatggc attgccgcgc
     9601 tccatgcgct tgatcacgcc ggtgacgaga tgctccttgc gcgagagaaa gtcgttgagg
     9661 atctgctcgc gttcggcgtc gcggatcttc tgcaggatca cctgcttcgc cgtttgtgcg
     9721 ccgatacgcc ccacttccag ggcaccgagc ggcttttcaa tgtaatcacc cggttgaatg
     9781 tcggggttct ccttctttgc gtcgctcagc acgatctggc gcgagggctc ttcgacttcc
     9841 ccctcgttca tcacctgcca gcgacggaaa aacgaaaagc cgcccgtcgc gcggtccacc
     9901 gaggcgcgca cgtcgatatc ttcatgaaaa cgcttcttgg tggcggatgc gagcgcaagc
     9961 tcaagcgcgc cgaacacgat ctccttgtcg acgttcttct cccgccccag ggcatccacc
    10021 agcaacaata tttcgcggct catcccgatc tcccgctata ggttcggcac caggcgcgcc
    10081 gtctccacgt tgccaatctc gagcgacagc agtctgccgt ccacttcgag ctgcaaacgt
    10141 cctgcgccag cctcgcgcac cacaccaacg aagttgcgct gcccgtccac cggcacgcgc
    10201 gtcctgacac gcgccctctg tccggcgaaa cgcacaaaat cccgttcgcc cttcaataac
    10261 cggtccaacc ccggcgacga gacttccagc cggtcgtaat tcacgttttc cactgccagc
    10321 acccggctca cctgatggct gaccgcggcg caatcatcca cggtgacacc acccggcttg
    10381 tcgatgtaaa tgcgcaataa ccgccccctg ccgggccgtt ccacgccgac cagttcgtag
    10441 cccatgccgc ccagcgtcgt ctccagtaac ccgttcaaat ccattacaaa tccgctccac
    10501 aaacaaaaaa tgggcgagcc gcccatttcc tgtttcccca gaaccggggg cgagaagtgt
    10561 agcaaaaatc acgcccaaat ccaataggtt ggataaaatc gccctagaaa caccctcgaa
    10621 acaccgcccc cgccatgccc ctgcccctgc gcgcgctcta cgtcgatttc aactcctact
    10681 tcgcctcggt tgagcagcaa ctgcggccgg agctgcgcgg caaaccggtg gcagtgctgc
    10741 cggtgatcgc cgacaccacc tgctgtatcg cggcgagtta cgaggccagg aagttcggga
    10801 tcaaaacagg aacgctggtg cgggaagcaa gacgcctgtg ccccggtctc cagctggtgg
    10861 aagcacagcc cgcgacctat gtcgaatacc accaccgcct cgtcgcggcc gtcgaatccc
    10921 gcacccatgt ggagaaggtg ctgtcgatcg acgagatgta ctgcgagttg ctggggcacg
    10981 agcggcggcg ggaccgagca ctggcgctgg ctcaggaaat caagcacgcc atcgcggcca
    11041 aagtgggcgc ggaactgcgc tgttcgatcg gcatcgcgcc taacgtgttc ctcgccaaga
    11101 cggcctcgaa cctgcagaag ccggacggcc tgacggtgat cgaacaaagg aacctcccgg
    11161 attgcctgtt cgggttcggg ttgcgcgacc tgtgcggcat tggcaggaac atggaaaaac
    11221 gcctcaacgc gctgggtatc gccagcgttg aggcgctgtg caaggcaggc aaaccggtcc
    11281 tgcgccgggc ctggggcggc gtcggcggcg agcgcatgta cgccaaactg cgcggcgaat
    11341 gggtggacga tttgccgacg gagcgatcga gcatcgggca ttcccacgtt ctgccgcctg
    11401 cactgcgctc gccccacgcg gctctttcgg tgttgcaccg tttgctgcag aaagcggcca
    11461 tgcggttacg aagctacggc ctgatcgcgg cggctttgca cgtgcatgtc gggcggctgg
    11521 acggttcttc ctgggaaaac cttatccgca tcgacccgac ccaggatacg ctgcggctgc
    11581 tcgatgcgct caaagccctg tgggcgggct atccggccgg gtcaaaagcc gctcccctga
    11641 aagtcggcgt cactctgctc gacctccacg agcaatccgg gcaaacacgg tcgttgttcg
    11701 acgacctcga aatacgcagc cggctcaatg cagccgtcga ccggctcaat ttgcgctacg
    11761 gcaagaacac cctctacttc ggcggcgctt atctggcgtt gagatccgcc cccatgcgga
    11821 tcgcgttcaa tcacataccg gatccgatgg tagaaggcga ctagcaccaa cacggatgcg
    11881 gaaaggttag cctgcgcctc aacaagccgg gcatgagaaa tgcactgtcg cgcaatagaa
    11941 aaagtgccgg cttgttgtcg tcccgaaagt agggcatctc ctcaggcaca cccacagaaa
    12001 cgaaaaagcc cgaccgcaag ggccgggctt ttccgtttca tgggtgcctg acgatgtcct
    12061 actttcgcac ggaatacacc gtactatcat cggcgctgag gcgtttcacg gccctgttcg
    12121 gaatgggaag gggtgggacc acctcgccat ggtcgtcaga cgtagcttag tgagctctca
    12181 gctgtcggcc atcagctttc agcaaaagct cgcgatcgga agaagtaaag tcgggcgatt
    12241 gtgtctggca aaccctggtt cacctgagcc ctggctgaca gctgattgct gaaagccgac
    12301 cgctcacggt tataggatca agccgcacgg gcaattagta ccggttagct caaaggatta
    12361 ctcctcttac acatccggcc tatcaacgcg gtagtcttcc gcgacccttc agggggatcg
    12421 agtccccggg aagtctcatc ttgaggcgag tttcccgctt agatgctttc agcggttatc
    12481 tcttccgcac atagctaccc ggcgatgcca ctggcgtgac aaccggtaca ccagaggtgc
    12541 gtccactccg gtcctctcgt actaggagca gcccccctca aacttccaac gcccactgca
    12601 gatagggacc aaactgtctc acgacgtttt gaacccagct cacgtaccac tttaaatggc
    12661 gaacagccat acccttggga ccggctacag ccccaggatg tgatgagccg acatcgaggt
    12721 gcca
//
LOCUS       DBNGWZ010000166         7908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2409327, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000166 DBNGWZ010000000
VERSION     DBNGWZ010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2409327"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..599)
                     /locus_tag="AC16CX_04975"
     CDS             complement(258..599)
                     /locus_tag="AC16CX_04975"
                     /inference="COORDINATES: protein motif:HMM:NF022671.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HIR3011898.1"
                     /translation="MLPEFEQRLIERLLARFYDRRVLAHVQHRTRLGHTIRGNSITLF
                     KIRPGFDDPAVWLRSPVAQFRYSSVTGLWSLYWRDRNRRWRPCEDMMDTRNFETLLAE
                     VDRDPTCIFWG"
     gene            complement(760..2934)
                     /locus_tag="AC16CX_04980"
     CDS             complement(760..2934)
                     /locus_tag="AC16CX_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3011899.1"
                     /translation="MTGKRLGAGLDAIDGLGLRPALLAWYRDLTTLRYDFPVVLVRQG
                     RDEACVQSLSGVVDGIIRDAAPREGERERFRKHLLRLEREIRALVAGSTAGRLSALWD
                     AAASRLGPGDESLEDSLGRARAALKADGELIDCDESMPARLLTHAWRTVQDQKARSFR
                     ENAGRLIQKLSDILRAAHAHSEAGQNEQSLKASVGTAYEDVFDFAVMSRFVKRTLPKT
                     PLPESRQRRIQWVLSVLGSQRFFPAPGESTGRSRAVEPYSFAFESCSSAVAAFHERLP
                     GLIEVAKAIAVGELEIEGHYLESKHDAFFAEFGEHMLGPKDLALFPDYLVCVRADTLQ
                     AAETAKLMEVLATGLPLKVLVQTDDILRESSLGDEFPAPGVSGARLASMAIGLNQVFV
                     LQSSASNLFQFRDRILRGLGYAGPAIFSVFSGAIRGAGGLPPYLAAAAAMQSRAFPAF
                     TYDPSAGPDWASRFCLEDNPQAERDWPVQDFAYEDEERQRISEQIAFTFIDFVACDPR
                     YAGHFARIPHADWNGSMIPVGEGLAGETRGLPEKLPYVLMVDRNDVLQKVIVDEKLIQ
                     EARRCREMWHSLQELGGIHNSHAERLLARERKAWEEQKQRAETPPREAKTAAGTAAAA
                     PAAVAAPAPAESIKEEKPSDEAYVETPRCTSCDECTQINNRMFAYDANKQAYIANPDA
                     GSYRDLVEAAESCQVSIIHPGKPRNPNEPGLDELIKRAEPFL"
     gene            complement(3045..4037)
                     /locus_tag="AC16CX_04985"
     CDS             complement(3045..4037)
                     /locus_tag="AC16CX_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3011900.1"
                     /translation="MTVPITECLLRLYEERHDLEDYAGGVPRWCTGCGDNAILAAVLR
                     LCRDEGLRPEKTVFVSGIGCSSRFPHYMGTYGFHGIHGRALPVAEGIKMARPDLHVFV
                     NTGDGDCCSIGAGHWIHAIRYNMDMTVLLHDNQIYGLTKMQASPTSPKGLRSNTTPRG
                     AYLDALNPLTVTLGVQNVSFVAQAVDWIPETLYEIISAAFHHKGFSFVRIIQRCPEWL
                     PNIFEPWLNDPSRILLLTHERGVRVSAGLAKVYKNQQVHDPANLDRAREIASRADPIP
                     VGILYRNADVPCYEEVRNADRLRTPGLIRAGLDAELDKFTVWPQEAETGRRPAA"
     gene            complement(4053..5963)
                     /locus_tag="AC16CX_04990"
     CDS             complement(4053..5963)
                     /locus_tag="AC16CX_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3011901.1"
                     /translation="MNDTSILQSDRKVAQLLRSPADEKTVTTLTEHVVEVVSDSGEGA
                     QRCGQSLGSIAARMGNGVWTVEIIPAEIQPPARSVAGASGNRVRLGTRRVTNGGDQAD
                     LVVAFNEQVLLGRVRAGELKPGCIILLESMWRDDRDPGITASYAETCAQLAAAGYKVH
                     EIPMERECRPIVDDPRRGKNMFVLGMLCSIYSLDLQLARDQVLFAFRKKDEEVIKSNH
                     KLLEAGYKWAEANLDFKFRIPAVRATEPQVVVNGNTALALGILASGMEVCAMYPITPA
                     TSASHYLSDVFEKAGGVVHQAEDEIAACAFAIGASYAGKCAVTITSGPGYSLKQEAIG
                     LAVMGEIPLVVVNVQRGGPSTGLPTKAEQGDLLTAIFGSHGDAPKVVMAASTIEDCFY
                     SIITARKIAETFNMVVIVLSDANLATSQQPFPRPQFSEAWLAPPVDQSALSEDVEPYG
                     WDAATGLARRFIPGQPNGMHCLTGLAHDRRSHVAYDPESNEEGIHYRSLKLAALQKSL
                     KTPPVFGGEEGDLLVVGWGSTKGAIEEAVERLRAEGCKVSSLHLRFLQPMPAGINEVL
                     HRFKKVMTIENNWCDRLEDEVIDDSNRRYSAVATLLRARYLVDVDCWGEVRGQPIRPG
                     TIYRVIREKLRK"
     gene            complement(6009..7514)
                     /gene="gltA"
                     /locus_tag="AC16CX_04995"
     CDS             complement(6009..7514)
                     /gene="gltA"
                     /locus_tag="AC16CX_04995"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR3011902.1"
                     /translation="MAKKKTIRTIPQERTAMREQRPGERAKNFKEVACGYRLEEALRE
                     ADRCLLCPDPACVEGCPVSIDIPGFIQRISAKDFRGAYDVISAANFLPAICGRVCPQE
                     SQCEGVCPVGETLEPVAIGRLERWVGDLAIAEGWANIPYIESNGFRIGIVGSGPAGMA
                     CAADMAKAGCEVTVYEAFHQPGGVLRYGIPDFRLPNEVIDAEVERLRKLGVNFECNTV
                     VGRLFTIEQMMDEMGYSAVFIGTGAGYPKLMGIPGDSLNGVLSANELLTRCNLMRARE
                     FPDYDTPLPLGRRIAVIGAGNTAMDAMRVCLRLGAEKVHCIYRRTKAECPARAEEIHH
                     AEEEGIEFHWLTNPVEVLDDGKGNVRGMRCIRMELGAPDESGRRSPVPVTGSEFEFAT
                     DMVVFAIGTNANPIIGQTSKLKLNKRGYIEADATLATSMAGVFAGGDIVTGAATVIEA
                     MGAGRSAARSMKAYLGIRDADAADSSAPSGGGARLFGIDLRERNFARIRVA"
     gene            complement(7604..>7908)
                     /locus_tag="AC16CX_05000"
     CDS             complement(7604..>7908)
                     /locus_tag="AC16CX_05000"
                     /inference="COORDINATES: protein motif:HMM:NF021903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR3011903.1"
                     /translation="VDGTGMCGGCRVRVGDRVRFACVDGPDFDAHQVDFDDLMTRLRR
                     FTNEEKAALARWSESCRMKDPLGPGSLNTALPAQPPGGLSNELLALPDPFDEVAGG"
BASE COUNT         1282 a         2628 c         2562 g         1436 t
ORIGIN      
        1 ccgcgaccag tgcgatagca actcgccgaa gagcgcagtg ccataatccc ggcggctgcg
       61 ccccgtctcc gccccaacac caaccccctc gccgagatcc ggtgcgccgc cctcgcgcaa
      121 atggaacaat cagccgcatc ggcttgacgg ggtaggcctg tcctgagccc gtcgaagcga
      181 cctgagcggt cgcgccgccc gctgccgcag cgagcgccag agcggtcatc gtgcacagag
      241 cgcgcttcac gttatcctca gccccagaag atgcatgtgg gatcgcgatc gacttcggcg
      301 agaagcgttt cgaagtttcg ggtatccatc atatcctcgc aaggccgcca ccgccgattc
      361 cggtcgcgcc agtagagcga ccaaaggccg gtgacggaac tgtaccgaaa ctgggcaacg
      421 ggggacctca gccataccgc aggatcgtcg aaacccggcc ggatcttgaa cagcgtgatg
      481 gagttgccgc ggatcgtgtg gccgagccga gtccggtgtt gaacgtgcgc gagcacgcgg
      541 cggtcgtaga aacgggcaag caatcgctca atgaggcgct gttcgaactc gggcagcacg
      601 gctggatctc atttcccgat accacctgtt tggcgacttc ttggttgatc attctcaccg
      661 gcaccgaacg gtaccttttt gcgccgccac tgcaaatcgc aaggggctgc tgccaactta
      721 caccgggtcc ggctgtccgg tcccgccgga agaattgcgc tacaggaacg gctcggcgcg
      781 cttgatcagt tcgtcgagtc ccggctcgtt cggattgcgc ggcttgcccg ggtggatgat
      841 ggatacctgg cagctctcgg cggcttccac gaggtcccgg tagctcccgg cgtccgggtt
      901 cgcgatatag gcctgtttgt tcgcatcgta ggcgaacatc cggttgttga tctgcgtgca
      961 ctcgtcgcag ctcgtgcagc gcggggtctc gacataggcc tcgtcggagg gcttttcctc
     1021 cttgatcgat tcggccggtg cgggagctgc gaccgccgcc ggtgctgccg cagcggttcc
     1081 ggctgcggtt ttcgcctcgc gcggcggggt ttccgcacgc tgcttctgtt cttcccacgc
     1141 cttcctttcg cgcgcaagca gccgctccgc gtgcgagttg tgaatgccgc cgagttcctg
     1201 caggctgtgc cacatctcgc ggcagcgacg ggcctcctgg atcagcttct cgtcgacgat
     1261 caccttctgc agcacgtcgt tgcggtcgac catcagcacg taagggagtt tttcgggcag
     1321 gcccctcgtt tcaccggcga gaccctcacc caccgggatc atgctcccgt tccaatcggc
     1381 atgcgggatc cgtgcgaaat gtccggcata gcgcgggtcg caggccacga agtcgatgaa
     1441 cgtgaaagcg atctgttccg aaatccgctg gcgctcctcg tcctcgtacg cgaaatcctg
     1501 gaccggccag tcgcgctccg cctggggatt gtcttccagg caaaaccgcg acgcccagtc
     1561 cgggcccgcg gacgggtcat aagtgaaggc gggaaacgcg cgcgattgca tcgcggcagc
     1621 cgcggccaga tagggcggga gccctccggc tccgcggatg gcgcccgaaa agacgctgaa
     1681 gatggcgggg ccggcatagc cgaggccccg caggatacgg tcccggaact ggaacaggtt
     1741 cgaggcgctc gactgcagga caaagacctg gttcagtccg atcgccatgc tggcgagtcg
     1801 cgcgcccgac acgccggggg cggggaattc gtcaccgaga gaagattccc gcaggatatc
     1861 gtcggtctgg accaggactt tcaggggcag cccggtggca agaacttcca tcagcttcgc
     1921 ggtttcggcc gcctgcagcg tgtcggcccg gacgcacacg aggtagtccg ggaacagggc
     1981 gaggtctttc gggccgagca tgtgctcgcc gaactcggcg aagaaggcat cgtgcttcga
     2041 ttcgaggtag tggccttcga tctcgagctc gccaaccgcg atcgccttgg cgacctcgat
     2101 caacccgggc agccgctcgt ggaaggccgc gacggcgctg ctgcagcttt cgaaggcgaa
     2161 agagtacggc tcaacggcgc ggctccgtcc ggtggactcg cccggcgcgg ggaaaaaccg
     2221 ctgcgacccg agaaccgaga gcacccactg gattcgccgc tgccggctct cgggcagcgg
     2281 cgtcttgggc agggtccgct tcacgaagcg cgacatcacc gcgaagtcga acacgtcttc
     2341 atacgcggtc ccgaccgatg ccttgaggct ttgctcgttt tgtccggcct ccgaatgcgc
     2401 gtgggcagcc cggaggatgt ccgaaagttt ctgtatcaag cggccggcat tctcccggaa
     2461 gctgcgcgcc ttctgatcct ggaccgtacg ccaggcgtga gtgagaaggc gcgccggcat
     2521 gctctcgtcg cagtcgatga gctcgccatc cgccttgagg gctgcgcggg cgcgacccag
     2581 gctgtcctcg agcgattcat cgcccggtcc gagccgactc gccgcggcgt cccagagcgc
     2641 cgagagccgc ccggcggtgc tccctgcgac gagcgcgcga atctcccgct ccagccgcag
     2701 gagatgcttt ctgaagcgtt cacgctcgcc ctcccgggga gcggcatcgc ggatgatgcc
     2761 gtcgaccacc ccggagagag actggacgca agcctcgtcc cggccttgcc ggactaatac
     2821 caccgggaag tcgtaacgca gggtggtgag gtcccgatac caggcgagca gcgcggggcg
     2881 aagcccgagc ccgtcgatcg cgtcaaggcc tgcgccgagc ctttttcccg tcaagtgaaa
     2941 ggcaacatgc gctttgaata actcgggttg catatcggtt ggtctggtgg ttatgagctg
     3001 ctccgggcag cgtcgcgcag gcgcgctcgc ggcgcctcac ccgcttacgc agccggccgc
     3061 cttcctgttt ccgcctcctg cggccataca gtgaacttgt cgagctcggc atcgaggccg
     3121 gcccggatga gccccggcgt gcgcaaccgg tcggcgttcc gcacttcctc gtagcaggga
     3181 acgtccgcgt tccggtaaag gatgcccacg ggaatcggat ccgcccgcga ggcgatctcg
     3241 cgcgcccggt cgaggttcgc cggatcgtgg acctgttgat tcttgtagac cttggcgaga
     3301 cccgcgctta cccgcacgcc gcgctcgtgg gtcagcagca gaatacggct cggatcgttg
     3361 agccagggct cgaagatgtt cggcagccac tccggacagc gctggatgat gcgcacgaag
     3421 gagaatccct tgtggtggaa agccgcggag atgatctcgt agagcgtttc cgggatccag
     3481 tcgaccgcct gcgccacgaa ggagacattc tgcacgccga gcgtgacggt cagtggattg
     3541 agcgcgtcga ggtaagcgcc gcgcggcgtc gtattgctgc gcagcccttt cggcgaggtc
     3601 ggcgaggcct gcatcttggt gagcccgtag atctggttgt cgtgcagcag caccgtcatg
     3661 tccatgttgt agcggatggc gtggatccag tgtccggcgc cgatgctgca gcaatcgccg
     3721 tcgccggtgt tcacgaacac gtgcaggtcc ggccgcgcca tcttgatgcc ctcggcgacc
     3781 ggcagcgccc gcccgtgtat gccgtggaac ccgtaggtgc ccatgtaatg gggaaagcgg
     3841 ctcgagcagc cgattcccga tacgaagacg gtcttctccg gtcgtagccc ctcgtcgcga
     3901 cacaaccgca gcacggccgc caggatggcg ttgtcgccgc acccggtgca ccagcgcggc
     3961 acgccgcccg catagtcctc caggtcgtgg cgctcctcgt agagccggag caggcactcg
     4021 gtgatgggaa cggtcatgtt cgctgcctct ccttacttcc gcaatttctc gcggatcacg
     4081 cggtagatcg tgccgggcct gatgggctgg ccccgcactt cgccccagca atcgacgtcg
     4141 accaggtaac gcgcccggag cagcgttgcg acggccgagt agcggcgatt gctgtcgtcg
     4201 atgacctcgt cctccaggcg gtcgcaccag ttgttctcga tcgtcatgac tttcttgaac
     4261 cggtgcagca cctcgttgat gcccgcgggc atgggctgga ggaacctcag atgcagggag
     4321 gataccttgc agccctccgc gcgcaggcgc tcgaccgcct cctcgatcgc gcccttggtg
     4381 ctgccccagc cgacgacgag caggtcgcct tcctcgccgc cgaacaccgg gggcgtcttc
     4441 agcgacttct gcagcgcggc gagcttcagg ctgcggtaat ggatgccctc ttcgttgctt
     4501 tccggatcat aggcgacgtg gctgcggcga tcatgcgcaa gcccggtcag gcagtgcatg
     4561 ccgtttggct ggccgggtat gaaacggcgg gcgaggccgg ttgcggcgtc ccagccgtac
     4621 ggctccacgt cctcggaaag cgcgctctgg tccaccggcg gggccagcca tgcctcgctg
     4681 aattgcggcc gtgggaaggg ttgctgggag gtcgccagat tcgcgtccga gagcacgatc
     4741 accaccatgt tgaaggtttc cgcgatcttg cgcgcggtga tgatcgagta gaagcaatcc
     4801 tcgatggtgc tcgccgccat gaccaccttc ggcgcatcgc cgtgactgcc gaagatggcc
     4861 gtcagcaggt cgccctgttc cgccttcgtc ggcagcccgg tgctggggcc gccgcgctgg
     4921 acgttcacga ccaccagcgg aatctcgccc atgaccgcga ggccgatggc ctcctgcttg
     4981 agcgaatagc cggggccgga ggtgatggtc accgcgcatt ttccggcgta cgaggcgccg
     5041 atcgcgaagg cgcaggcggc gatctcgtct tcggcctgat ggaccacgcc gcccgctttt
     5101 tcgaacacgt cgctcaaata gtgcgacgcc gaggtggcgg gcgtgatggg atacatggcg
     5161 cagacctcca tgcccgacgc gagaatgccg agcgccaatg ccgtgttgcc gttcaccacg
     5221 acttgcggct cggtcgcgcg caccgccgga atccggaact tgaagtcgag gttggcctcc
     5281 gcccacttgt agcccgcttc cagcagcttg tggttcgact tgatgacctc ctcgtccttc
     5341 ttgcggaagg cgaacaggac ctggtcccgc gccagttgca ggtccaggct gtagatgctg
     5401 cagagcatgc ccaaaacgaa catgttcttg ccgcgccgtg gatcgtcgac gataggccgg
     5461 cattcccgtt ccatgggaat ctcgtgcacc ttgtagccgg ccgccgcgag ctgagcgcag
     5521 gtctcggcat acgatgccgt gattcccggg tcgcggtcgt cccgccacat gctttcgagg
     5581 agaatgatgc agccgggctt gagctcgccc gcgcgcaccc ggccgagcag cacctgctcg
     5641 ttgaaagcca ccacgaggtc cgcctgatca cccccgttgg tgacccggcg cgtcccgagg
     5701 cgcacgcggt tgccgcttgc tccggcgacg ctgcgcgccg gaggctggat ttcggcgggg
     5761 atgatctcga ccgtccatac gccgttgccc atgcgcgcgg cgatcgagcc gagcgactgg
     5821 ccgcagcgtt gcgcaccctc gccggaatca ctgaccacct cgacgacatg ctccgtcagc
     5881 gtggtgaccg ttttttcgtc tgcgggtgac cggagaagct gggcgacctt cctgtcggat
     5941 tgcaatatgg aggtgtcgtt catcgttttt gtgcgatcgt tgtcgagccg tttgtccggt
     6001 ttccaagcct aagcgacgcg gatgcgggcg aaattgcgtt ccctgagatc gatgccgaac
     6061 agtcgggcgc cgccgccgct cggcgcactg gaatcggcgg cgtcggcatc gcggatcccg
     6121 agataggcct tcatgctgcg tgccgcgcta cggcccgcgc ccatggcctc gatcaccgtc
     6181 gcggctccag tgacgatatc gccgcccgcg aatacccccg ccatggaggt ggccagcgtc
     6241 gcgtccgcct cgatgtagcc gcgcttgttg agcttgagct tcgaggtctg gccgatgatc
     6301 ggattcgcgt tcgtcccgat cgcgaatacg accatgtcgg tcgcgaattc gaactcgctg
     6361 ccggtgacgg gaacggggct gcgccggccg gactcgtccg gcgcgcccag ctccatgcgg
     6421 atgcaacgca tgccgcggac gttgcccttg ccgtcgtcga ggacctcgac cggattcgtc
     6481 agccagtgaa actcgatccc ttcttcttcg gcgtggtgga tctcctcggc gcgcgccggg
     6541 cactccgcct tggtgcggcg gtagatgcaa tgcaccttct ccgcgccaag cctcaggcag
     6601 acccgcatcg cgtccatcgc cgtattgccg gcgccgatga cggcgatgcg ccggccgagc
     6661 gggagggggg tgtcgtagtc ggggaactcc cgggcgcgca tcaggttgca gcgcgtcagc
     6721 agctcgtttg ccgacagaac gccgttcaac gaatcgcccg ggatccccat gagcttgggg
     6781 tagcccgcgc cggttccgat gaacacggcg ctatagccca tctcgtccat catctgctcg
     6841 atcgtgaaca ggcgtccgac caccgtgttg cactcgaaat tgacgccgag cttgcgcagc
     6901 ctctcgactt cggcgtcgat cacttcattc gggaggcgga aatcggggat gccatagcgc
     6961 aggacgccgc cgggttgatg gaaggcctcg tagaccgtga cctcgcatcc cgccttggcc
     7021 atgtcggccg cgcaggccat gccggcgggt ccggagccga cgatcccgat ccggaagccg
     7081 ttggattcga tataggggat gttggcccag ccctccgcga tggcgaggtc gccgacccaa
     7141 cgctcaaggc ggccgattgc gaccggttcc agcgtctcgc ctaccgggca aacgccttcg
     7201 cactggctct cctgcgggca aacccgaccg cagatggcgg gcaggaaatt ggccgcgctg
     7261 atgacatcgt aggcgccacg gaaatccttc gcgctgatcc tctggatgaa gcccgggata
     7321 tcgatgctga ccgggcagcc ttccacgcaa gccggatcgg ggcacagcag gcagcggtcc
     7381 gcttccctca gcgcttcttc cagacggtag ccgcaggcca cctccttaaa attctttgcg
     7441 cgctcgccag gccgctgctc gcgcatcgcg gtccgttcct gcggaatcgt gcgtatcgtc
     7501 ttcttcttgg ccatcctata accctcgaga aaccgctgaa ccctcccggt ggcgccgcac
     7561 cggcacgcgt gccgcgcgac ggcagccgtc acgtcggcgc acgctaaccg cccgccactt
     7621 cgtcgaatgg atcgggtagt gcaagcagtt catttgataa gccccccggc ggctgcgccg
     7681 gcaacgccgt gttcagtgac ccgggaccaa gcgggtcttt catgcggcag ctttccgacc
     7741 atcgcgccag cgccgccttc tcctcattgg tgaagcgtcg caaccgggtc atcagatcgt
     7801 cgaagtcaac ctggtgagcg tcaaagtccg ggccgtccac gcacgcgaac ctcacgcggt
     7861 cgccgacgcg gacgcggcag ccgccgcaca ttccggtgcc gtcgacca
//
LOCUS       DBNGWZ010000167         1716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3417317, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000167 DBNGWZ010000000
VERSION     DBNGWZ010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3417317"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="AC16CX_05005"
     CDS             complement(<1..584)
                     /locus_tag="AC16CX_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024571.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3011904.1"
                     /translation="MRYLRRELESRVAAAARQFPAVVLTGPRRAGKTFLLRHLLPKAS
                     YHLFEDPDLVARFRHDPQGFLDGVRPPAILDEIQNVPEVFNFIRSRIDRTPRRTGQWF
                     LTGSQETGLMRRVTESMAGRAAVLQLYPMSVRETPKVSLLRGGYPEVLARPRAASLWF
                     SSYLQTYLERDVRAISAVHDLAMFRRFLALLASR"
     gene            complement(621..1406)
                     /gene="pyrF"
                     /locus_tag="AC16CX_05010"
     CDS             complement(621..1406)
                     /gene="pyrF"
                     /locus_tag="AC16CX_05010"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3011905.1"
                     /translation="MTDARAAAKSKLIFALDVPSESDARRLVALLKDHVGLFKVGLEL
                     FVAAGPSILDIISDQTDAGIFLDLKFHDIPETVKAASRAAERHRVRFLTVHCDQRVRL
                     AEPARTAPAGPRLLGVTALTSLGEADLRTAGWYAKDLTVEQLVLLRAQTAFDAGCAGV
                     VCSGNEARAIKTRFGPEFLVVAPGIRPAWAAVKGDDQRRAAAPAEAIAQGADYLVVGR
                     PIRTAPDPVKAAERVIEEIAASLPSIPSSQGATLSAATRTKEK"
     gene            complement(1435..>1716)
                     /locus_tag="AC16CX_05015"
     CDS             complement(1435..>1716)
                     /locus_tag="AC16CX_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR3011906.1"
                     /translation="RRIAAVFDQKINPGLAQHGGKVALVDLKDTIAYVEMSGGCQGCS
                     MAIKTLRNGIMRVLAEEFPELTDVVDTTDHSGGANPYFTGDRQGDSPAL"
BASE COUNT          281 a          542 c          609 g          284 t
ORIGIN      
        1 tgtcgactgg ccagcagggc gaggaagcgg cggaacatgg cgagatcatg caccgcactg
       61 atggcgcgga catcgcgctc caggtaggtc tgaaggtacg agctgaacca gagcgaagcc
      121 gcacgggggc gcgccaagac ttcgggatag cccccgcgca gcaggctgac cttgggcgtc
      181 tcgcgcaccg acatcgggta gagttgcagc accgccgcgc ggccggccat ggactcggtg
      241 acgcggcgca tcaagccggt ctcctgcgag ccggtcagaa accactgccc cgtccggcgg
      301 ggcgtgcgat cgatgcggct gcggatgaaa ttgaagacct cgggtacgtt ttgaatttcg
      361 tccaagatgg ccggcgggcg taccccatcg agaaagcctt gcgggtcgtg ccggaaccgc
      421 gccacgagat ccggatcctc gaacaggtga tagctggcct tgggcagaag atgccgcagg
      481 agaaaggtct tgccggctcg gcgtggcccc gtcagcacta cggcggggaa ctgacgcgcc
      541 gctgccgcga cgcgcgattc gagttcgcga cggaggtagc gcatggatga gtattttaaa
      601 ctaattgctt aaaatactca ctatttttcc ttcgtccgtg ttgccgccga gagcgtggct
      661 ccttgcgacg acggtatgct cggaaggctt gccgcgattt cctcgatcac ccgctcggcg
      721 gctttgaccg ggtcgggcgc ggtgcggatc gggcggccga cgacgaggta atcggcgccc
      781 tgcgcgatcg cctcggcggg cgcggcggcg cgccgttgat cgtcgccctt gaccgccgcc
      841 cacgcggggc ggatgccggg ggcgacgacg aggaactcgg ggccgaaacg tgttttaatc
      901 gcgcgggctt cgttccccga acagacgacg cccgcgcagc cggcgtcgaa ggcggtctgg
      961 gcgcgcaaca gcacgagctg ctcgacggtc aggtccttcg cgtaccagcc cgcggtccga
     1021 aggtcggcct cgccgaggct cgtgagggcg gtcaccccga ggagccgggg tcccgccggc
     1081 gcggtccgag ccggctcggc gagtcgaacg cgctggtcgc agtgcacggt gaggaaacgc
     1141 acccggtgcc gttcggccgc ccgcgaggcg gccttcacgg tttcagggat gtcgtgaaat
     1201 ttcaggtcga ggaaaattcc ggcgtcggtc tgatcggaga tgatgtccaa gatcgacggg
     1261 cccgccgcga cgaacaattc caggccgacc ttgaacaggc cgacgtggtc tttcagcagc
     1321 gcgacgaggc gtcgggcgtc ggattccgac ggaacatcca gcgcgaagat cagcttgctc
     1381 ttggccgcgg cgcgcgcgtc ggtcattcgg catctcccct ggggcggcgg agcgtcacag
     1441 cgcgggcgag tctccctgtc ggtcgcccgt gaagtaggga ttggcgcctc ccgaatgatc
     1501 ggtcgtgtcc accacgtcgg tgagctccgg gaactcctcg gcgaggacgc gcatgatgcc
     1561 gttccgcagt gtcttgatcg ccatgctgca cccctggcag ccgcccgaca tctcgacgta
     1621 ggcgatcgtg tccttgaggt cgaccagcgc gaccttgccg ccgtgctgcg cgaggccggg
     1681 gttgatcttc tggtcgaaca cggcggcgat ccggcg
//
LOCUS       DBNGWZ010000168         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1527888, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000168 DBNGWZ010000000
VERSION     DBNGWZ010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1527888"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC16CX_05020"
     CDS             <1..522
                     /locus_tag="AC16CX_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3011907.1"
                     /translation="MDGLDVCRVLRREGSTPIIMLTARVEESDKLVGLELGADDYLTK
                     PFSPRELVARVRTVLRRASGPAAPAEVIRAGALTLDRARYHALLPDREGHDREVALTP
                     TEFEILAALAGQPGRIFSRAQLLTVARGVAFESYERAIDSHIRNLRRKIEPNEGEPRY
                     IITVHGVGYKFAE"
     gene            527..>1604
                     /locus_tag="AC16CX_05025"
     CDS             527..>1604
                     /locus_tag="AC16CX_05025"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3011908.1"
                     /translation="MTHFPDPHGSVGHVRRRLFLLLVQAFGAVVLLTLVLLIAVVGLV
                     LGNASRFTSAFKPPAAYALEAYYLVRGSWDGVESLPRDIPESGESEWARGLLLDAAGR
                     VVLDHGRADSPRVGQLYSPTRPGLPFPLEVGGASIGTFVFEQRADWGLVRVFVALLPP
                     LVVISFFTGLLTLLIGLLLMRRVVTPLADVIAAAQAVAAGDLSSRVQVRGPGDLRSLS
                     DSFNRMADALERNDRERRNLLADVAHELRTPLTIIRGKLEGMLDGVYPADEAHVAPVL
                     EETYVLERLVEDLRLLTLAETRQLHFDREPVDLGELAERAAGLFEAEAAERGIAVRVQ
                     VEPGLPPILADPQRMGQVIGNLMSN"
BASE COUNT          228 a          593 c          528 g          255 t
ORIGIN      
        1 atggacggac tggacgtgtg ccgcgtgctg cggcgagagg gcagcactcc catcatcatg
       61 ctcacggcgc gcgtcgagga aagcgacaaa ctcgtagggc tggagttggg cgcggacgac
      121 tatctcacca agccgttcag cccgcgcgag ctcgtggccc gtgtgcggac cgtactgcgg
      181 cgcgcctccg gccctgccgc gcccgccgag gtcatccgcg ccggggcgct caccctcgac
      241 cgcgcccgct accacgccct cctgcctgac cgtgagggcc acgaccgtga ggtcgcgctc
      301 acgccgaccg agttcgaaat cctcgccgcg ctggccggcc agccgggccg cattttctcg
      361 cgcgcgcaac tcctcaccgt cgcgcgcggc gtcgcctttg agagttacga acgcgcgatt
      421 gactcgcaca tccgcaacct gcgccggaag atcgagccga acgaaggcga gccgcgctac
      481 atcatcaccg tgcacggcgt cgggtacaag ttcgcggagt gacggtgtga cgcactttcc
      541 tgatccccac ggctctgtcg gccacgtccg gcgccgcctg ttcctgctgc tggtgcaggc
      601 ctttggcgcg gtcgtgctgc tcacgctcgt cctgctcatc gccgtcgtcg ggctggtact
      661 cggcaacgcc tcgcgcttca cctcggcgtt caagccgccc gccgcctacg cgctcgaagc
      721 gtactacctg gtgcgcggaa gctgggacgg cgtcgagagc ctgccgcgcg acattccaga
      781 gagcggcgaa agcgagtggg cgcgcggcct gctgctcgac gccgccgggc gcgtcgtgct
      841 cgatcatggg cgggccgact cgccccgcgt gggccagctc tactcgccca cccggcccgg
      901 cctccccttc ccgcttgaag tcgggggcgc ttcgattgga acgttcgtct tcgagcaacg
      961 cgccgattgg ggcctcgtgc gcgtcttcgt ggccctgctg ccgcccctcg tcgtcatctc
     1021 gttcttcacc ggactgctca ccctgctcat cggactgctg ctcatgcggc gcgtggtcac
     1081 gccgctggcc gacgtgatcg cggcagcgca ggcggtggcg gcgggcgacc tctcgtcgcg
     1141 cgtgcaggtg cgcggccccg gcgacctgcg cagcctgagc gactccttca accgcatggc
     1201 cgacgcgctc gaacgcaacg accgcgagcg ccgcaacctg ctcgccgacg tggcccacga
     1261 gctccgcacg ccgctcacca tcattcgcgg aaagctggag ggcatgctgg acggcgtcta
     1321 tcccgccgac gaggcgcacg tcgcgcccgt gctcgaagaa acctacgtgc tcgaacggct
     1381 cgtggaagac ctgcgcctgc tgacgctggc cgagactcgc caactgcact tcgaccgcga
     1441 gccggttgac ctcggcgaac tggcggagcg cgccgccgga ttgttcgagg ccgaggccgc
     1501 cgaaaggggc atcgcggtca gagtgcaagt cgaacccggg ctgccgccca tcctcgccga
     1561 cccgcagcgc atgggtcagg tcatcggcaa tctgatgagc aacg
//
LOCUS       DBNGWZ010000169         9624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2284335, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000169 DBNGWZ010000000
VERSION     DBNGWZ010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9624
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2284335"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="AC16CX_05030"
     CDS             complement(<1..1435)
                     /locus_tag="AC16CX_05030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3011909.1"
                     /translation="MKALLDLFRQVTQEEEFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTLSKVRRERM
                     GHIELASPVAHIWFLKSLPSRMGMVLDMTLRDIERALYFEAYVVIDPGMTPLKRAQLM
                     TEDDYLAKVEEFGDDFTAMMGAEGIRNLLRSLDLRKEIDTLREDLAATSSDTKIKKIA
                     KRLKVLEAFHKSGIKPEWMILEVLPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIKGK
                     SGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPYIFHKLEVMGLAT
                     TIKAAKRMVEDQEPIVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAFNADFDGDQMAVHVPLSLEAQM"
     gene            complement(1505..5611)
                     /gene="rpoB"
                     /locus_tag="AC16CX_05035"
     CDS             complement(1505..5611)
                     /gene="rpoB"
                     /locus_tag="AC16CX_05035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3011910.1"
                     /translation="MAYSFTEKKRIRKSFAKRDVVLPVPFLLATQLDSYAAFLQANVV
                     PESRKTEGLQAAFKSIFPIESHSGVAELDFVSYALGEPPFDVKECQQRGLTYAAPLRA
                     KVGLVIRDKEAPKVKQKDGRDRHPVKERKEQEVYMGEIPLMTSTGSFVINGTERVIVS
                     QLHRSPGVFFEHDRGKTHSSGKLLFSARVIPYRGSWLDFEYDPKDYLYFRVDRRRKMP
                     VTILLKALGYTPEKILAEFFAFDTFQIGKDGVEFELVPERMRGEIARFDITTKGGKPI
                     VAKDKRITVKHVREMEQAGLHRIAVPEEFVIGRVLAHNVVDKGTGEVLANANDEITEQ
                     LLAKLKEAGAARIQTIYTNDLDQGPYISQTLRIDETADQLAAQVAIYRMMRPGEPPTE
                     EAVKTLFHRLFFSEDTYDLSKVGRMKFNRRVGRVELTGTGTLSADDIVAVIKILVDLR
                     NGKGEIDDIDHLGNRRVRSVGELAENQFRAGLVRVERAVKERLSQAESENLMPHDLIN
                     AKPVSAAVREFFGSSQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHP
                     THYGRVCPIETPEGPNIGLINSLALYARTNEYGFLETPYRKVRDARVTDEIHYLSAIE
                     EGQYMIAQANAALDRQNRFTDELVSCRHQNEFTMSAPDRIDYMDVAPSQAVSVAASLI
                     PFLEHDDANRALMGSNMQRQAVPCLRPEKPLVGTGLERTVAVDSGTAVQARRGGVVDY
                     VDASRIVVRVNDEETALGEVGVDIYNLVKYQRSNQNTNINQRPLVKVGDRIARGDVVA
                     DGASTDTGELALGQNMLVAFMPWNGYNFEDSILISERVVGDERFTSIHIEELSVVARD
                     TKLGPEEITRDISNLSESQLANLDESGIIYIGAEVEAGDVLVGKVTPKGETQLTPEEK
                     LLRAIFGEKASDVKDTSLRVPSGMSGTVIDVQVFTREGIERDKRAQQIIDDELKRYKK
                     DLADQLRIVENDAFERLERLLIGKTANGGPKKLARGSKITKAYLTEVERHGWFDIRVA
                     NDKIAAQLESIKESLAQTKALFDKAYDEKKKKLTQGDELAPGVQKMVKVYLAVKRRLQ
                     PGDKMAGRHGNKGVISKIVPVEDMPYMADGTPVDIVLNPLGVPSRMNVGQILETHLGW
                     AAKGLGLKIGELLKAQAKAAEVRRLLNRIYSSGARAEDLESLGDEDVMRLAENLKGGV
                     PFATPVFDGATEREIKEMLDLAGLPRTGQVTLHDGRSGDPFERSVTVGYMHVLKLHHL
                     VDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSD
                     DTEGRRKVYESIVKGDHRIEAGMPESFNVLVKEIRSLAIDIDLDHNRY"
     gene            complement(5768..6151)
                     /gene="rplL"
                     /locus_tag="AC16CX_05040"
     CDS             complement(5768..6151)
                     /gene="rplL"
                     /locus_tag="AC16CX_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3011911.1"
                     /translation="MAIAKAEILDAIANMTVLELSQLIKEMEDKFGVSAAAATVAVAA
                     PAAGGAAAAAAEEQTEFTVMLNSAGANKVNVIKVVRAVTGLGLKEAKDLVDGAPKPVK
                     EAVSKADAESILKQLTEAGATGEIK"
     gene            complement(6214..6744)
                     /gene="rplJ"
                     /locus_tag="AC16CX_05045"
     CDS             complement(6214..6744)
                     /gene="rplJ"
                     /locus_tag="AC16CX_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3011912.1"
                     /translation="MGLNLEQKQAVVAEVSSQLAKAQAVILAEYRSIPVKEMTELRKK
                     ARGSGVYLRVLKNTLARRAVADTPFKGLTDKMVGPLAYGISSDPVAVAKVLQEFAKDN
                     EKFVIRAGAMPNVLMTAREVAELARMPSRRELLAKLVATMQAPVAKFVRTLNEVPGKF
                     ARTLAAVRDQKQRQAA"
     gene            complement(7018..7707)
                     /gene="rplA"
                     /locus_tag="AC16CX_05050"
     CDS             complement(7018..7707)
                     /gene="rplA"
                     /locus_tag="AC16CX_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3011913.1"
                     /translation="MASKRYNAMRAKVDRAKSYPVQEALKLVKDGATAKFDESVDVSI
                     NLGIDASKSDQTVRGSVVLPQGTGKSVRIAVFAQGDKAKQARDAGADIVGFEDLANDI
                     KGGKMDFDVVIASPDAMRVVGPLGQILGPRGLMPNPKVGTVTQDVATAIRNAKAGQVQ
                     YRADKTGIVQCTIGRASFTVDALAENLKTLVGAVNKSKPSSVKGIYLKKVSVSSTMGV
                     GVRVDQASLQQ"
     gene            complement(7708..8139)
                     /gene="rplK"
                     /locus_tag="AC16CX_05055"
     CDS             complement(7708..8139)
                     /gene="rplK"
                     /locus_tag="AC16CX_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3011914.1"
                     /translation="MAKKVVGIIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQKMEPGLPVPVVITAYQDKSFTFVMKTPPASVLIRKAAKIEKGSSRPNTEKVGKIT
                     RAQAEEIAKAKLPDLTAADLDAAVRTIAGSARSMGVDVEGL"
     gene            complement(8240..8773)
                     /gene="nusG"
                     /locus_tag="AC16CX_05060"
     CDS             complement(8240..8773)
                     /gene="nusG"
                     /locus_tag="AC16CX_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3011915.1"
                     /translation="MSKKWYVVHAYSGFEKSVQRTLQDRILRSGMQDKFGQILVPVEE
                     VVEMKSGQKAISERKFFPGYVLVEMEMTDDTWHLVKNTPKVTGFVGGTATKPTPISEK
                     EVQNILHQIQEGVEKPRPKVLFEVGEAVRVKEGPFADFHGNVEDVNYDKNKLRVSVTI
                     FGRSTPVELDFSQVEKA"
     gene            complement(8773..9120)
                     /gene="secE"
                     /locus_tag="AC16CX_05065"
     CDS             complement(8773..9120)
                     /gene="secE"
                     /locus_tag="AC16CX_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3011916.1"
                     /translation="MADKIKIAAALLLVAAGVGGFYFLDQSPMILRVVAVLAGLVAGA
                     VTFWMSAPGKEFHGFAQESVTETKKVVWPTRKETLQTTGVVFAFVVVMAVFLWAVDAS
                     LLWIVKKLLGQSG"
     gene            complement(9216..9291)
                     /locus_tag="AC16CX_05070"
     tRNA            complement(9216..9291)
                     /locus_tag="AC16CX_05070"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9256..9258),aa:Trp,seq:cca)
     gene            complement(9333..>9624)
                     /locus_tag="AC16CX_05075"
     CDS             complement(9333..>9624)
                     /locus_tag="AC16CX_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR3011917.1"
                     /translation="ITPHTKFTAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGSI
                     ELAAGTEMVMPGDNVSITVSLIQPIAMEEGLRFAIREGGKTVGAGVVAKVIE"
BASE COUNT         1566 a         3195 c         2865 g         1998 t
ORIGIN      
        1 ccatctgcgc ctccagcgac agcggcacgt gcaccgccat ctggtcgccg tcgaagtcgg
       61 cgttgaacgc ggcgcacacc agcgggtgca gctggatcgc cttgccctcg atcagcaccg
      121 gctcgaaggc ctggatgcca agacggtgca gcgtcggcgc gcggttcagc atcaccggat
      181 gttcgcggat cacctcttcc aggatgtccc acacgatggg ttcctggtcc tccaccatcc
      241 gcttcgccgc cttgatggtg gtcgcgagcc ccatcacttc cagcttgtgg aagatgtagg
      301 gcttgaacag ctcgagcgcc atcagcttgg gcaggccgca ctggtggagc ttcagttggg
      361 gccccaccac gatcaccgag cggccggaat aatccacgcg ttttcccagc aggttctggc
      421 ggaagcgccc gctcttgccc ttgatcatgt cggcgagcga cttcaacggc cgcttgttgg
      481 cgccggtcat ggccttgccg cggcggccgt tgtcgagcag cgaatccacc gcttcctgca
      541 gcatgcgctt ttcgttgcgc acgatgatct cgggggcctt gagctccagc aggcgcttga
      601 ggcgattgtt gcggttgatg acgcgccggt agaggtcgtt caagtccgag gtggcgaagc
      661 gcccgccgtc gagcggcacc agcggccgca gctcaggcgg cagcaccggc agcacctcga
      721 ggatcatcca ttccggcttg atgcccgact tgtggaacgc ttccaacacc ttgaggcgct
      781 tggcgatctt cttgatcttg gtatccgagc tggtggcggc gagatcctcg cgcaacgtgt
      841 cgatttcctt cctgagatcg aggctgcgca acagattgcg aatgccttcc gcgcccatca
      901 tcgcggtgaa gtcgtcgccg aattcctcga ccttggcgag gtagtcgtcc tccgtcatga
      961 gctgggcgcg cttcaacggc gtcatgcccg ggtcgatcac gacgtaggcc tcgaagtaga
     1021 gcgcgcgctc gatgtcgcgc agcgtcatgt cgagcaccat gcccatgcgc gagggcaggc
     1081 tcttgaggaa ccagatgtgc gcgaccgggc tcgcgagctc gatgtggccc atgcgctcgc
     1141 ggcgaacctt ggacagtgtc acctcgacgc cgcacttctc gcagatcacg ccgcgatgct
     1201 tcaaccgctt gtacttgccg cacaggcact cgtagtcctt caccgggccg aagatcttgg
     1261 cgcagaacag gccgtcgcgc tcaggcttga aggtgcggta gttgatggtc tcgggcttct
     1321 tcacctcgcc gtacgaccac gagcggatct tctcgggcga agcgagcccg atcttgatcg
     1381 cgtcaaactc ttcttcctgc gtaacttgcc tgaacagatc cagcagtgct ttcatctcgg
     1441 ctcctaggtg atgtgcagcg ggcgatttcc tgccccttca ctcctcactc ttcacccttc
     1501 aatgtcaata acggttgtgg tcgagatcga tatcgatcgc gagcgagcgg atttccttca
     1561 ccagcacgtt gaacgattcc ggcataccgg cttcgatgcg gtgatcgccc ttgacgatgg
     1621 attcatagac cttgcgccgg ccttccgtgt cgtcggattt gacggtcagc atctcctgca
     1681 gcgtgtaggc agcgccgtag gcttccagcg cccacacctc catctcgccg aagcgctggc
     1741 cgccgaactg cgccttgccg ccgagcggct gttgcgtaac caggctgtag ggaccggtcg
     1801 agcgcgcgtg catcttgtcg tccaccaggt gatgcagctt cagcacgtgc atgtaaccca
     1861 ctgtcaccga ccgctcgaag gggtccccgg agcgcccgtc gtggagggtg acctgtccgg
     1921 tgcgcggcag gcccgccaga tcgagcattt ccttgatctc cctctcggtg gcgccgtcga
     1981 acaccggcgt ggcgaacgga acgccgccct tcaggttctc tgccaggcgc atgacatcct
     2041 cgtcgccgag cgactcgagg tcctcggcgc gcgcgccgct cgagtagatc cggttgagga
     2101 gcctgcgtac ctcggcggcc ttcgcctgcg ctttcagcag ctcgccgatc ttcagcccta
     2161 ggcctttagc ggcccacccg agatgggtct cgaggatttg gcccacgttc atgcgcgaag
     2221 gcacgccgag ggggttcaat acgatgtcga ccggcgtgcc gtccgccatg tacggcatgt
     2281 cctccaccgg cacgatcttg gagatgacgc ccttgttgcc atggcgtccc gccatcttgt
     2341 cgcccggttg cagccggcgc ttgaccgcaa ggtagacctt taccatcttc tgcacgcccg
     2401 gggccagttc gtcgccctgg gtgagtttct tcttcttctc gtcgtacgcc ttgtcgaaca
     2461 gcgccttggt ctgggcgagg ctctccttga tgctctccag ttgcgccgcg atcttgtcgt
     2521 tcgctacgcg gatatcgaac cagccgtggc gctcgacttc ggtcaagtag gccttggtga
     2581 tcttggaacc ccgggcgagc ttcttcggcc cgccgttggc cgtcttgccg atcagcagcc
     2641 gctcaagccg ctcgaaggcg tcgttctcga cgatgcgcaa ctggtccgcg aggtccttct
     2701 tgtagcgctt cagctcgtcg tcgatgatct gttgcgcgcg cttgtcgcgc tcgatgccct
     2761 cgcgggtgaa cacctgcaca tcgatcacgg tgccggacat gccggaaggc acgcgcaagc
     2821 tggtgtcctt cacgtcggag gctttttcgc cgaagatcgc gcgcaggagt ttttcttccg
     2881 gggtgagctg tgtctcgcct ttcggcgtca ccttgcccac cagcacgtcg ccggcctcga
     2941 cctccgcccc gatgtagatg atcccggact cgtcgagatt ggcgagctgg gactcggaca
     3001 ggttggagat gtcgcgcgtg atctcctccg ggccgagctt ggtgtcgcgc gccacgacgg
     3061 aaagctcctc gatgtggatc gaggtaaagc gctcgtcccc gacgacccgc tccgagatca
     3121 ggatcgagtc ctcgaagtta tagccgttcc acggcatgaa cgcgaccagc atgttctggc
     3181 ccagcgcaag ctcgccggta tcggtcgagg cgccgtcggc caccacgtcg cccctggcga
     3241 tgcggtcgcc gaccttgacc agcggccgct ggttgatgtt ggtgttctgg ttggaacgct
     3301 ggtacttgac caggttgtag atgtccacgc ccacctcgcc cagcgccgtc tcttcgtcgt
     3361 tcacccgcac cacgatgcgc gaagcatcca cgtaatccac cacgccgccg cggcgcgcct
     3421 gcacggcggt gcctgaatcc acggctacgg tgcgctcgag tcccgtgccc accaagggct
     3481 tctccgggcg caggcacggt accgcctggc gctgcatgtt ggagcccatc agcgcgcggt
     3541 tggcgtcgtc gtgctcgagg aaggggatga gggaagccgc gaccgatacc gcctgcgagg
     3601 gcgcgacgtc catgtagtca atgcggtcgg gagccgacat cgtgaattca ttctgatggc
     3661 ggcaggaaac cagctcgtcg gtgaagcggt tctgcctgtc gagcgcagcg ttcgcctgcg
     3721 cgatcatgta ctggccttcc tcgatcgccg aaaggtaatg gatctcgtcg gtgacgcgcg
     3781 catcccgcac cttgcgatac ggcgtctcga gaaagccgta ctcgttggtg cgggcgtaca
     3841 gcgcgagcga gttgatcagg ccgatgttcg ggccttccgg cgtctcgatc gggcatacgc
     3901 gtccatagtg cgtgggatgc acgtcgcgta cctcgaaacc ggcgcgttcg cgcgtgagac
     3961 cgccgggacc cagggcggac acgcgccgtt tgtgcgtgat ttccgagagc gggttggtct
     4021 ggtccatgaa ctgcgagagc tgcgaggagc cgaagaactc gcggaccgcg gccgataccg
     4081 gcttggcgtt gatgaggtcg tgcggcatca ggttctcgga ttcggcctgc gagagccgct
     4141 ccttcactgc gcgctcgacc cgcaccaatc ccgcccggaa ctggttctcg gcgagctcgc
     4201 ccaccgagcg cacgcgccgg ttgccgaggt ggtcgatatc gtcgatctcg cccttgccgt
     4261 tgcgcaggtc caccaggatc ttgatgaccg caacgatgtc atcggccgag agcgtaccgg
     4321 tgcccgtgag ctcgacgcgg cccacccggc ggttgaactt catgcggccg accttggaga
     4381 gatcgtaggt gtcttcgctg aagaacaggc ggtggaacaa cgtcttcacc gcttcctcgg
     4441 tcggcggctc gcccggccgc atcatgcgat agatcgcgac ctgagccgcc agctggtccg
     4501 cggtctcgtc gatgcgcagc gtctgcgaaa tgtacggtcc ctggtccagg tcattggtgt
     4561 agatggtctg gatcctggcg gcgccggcct ctttgagctt ggcaaggagc tgctcggtga
     4621 tctcgtcgtt ggcgttggcg agaacttcgc ccgtgccctt gtccacgacg ttgtgcgcca
     4681 gcacccggcc gatcacgaat tcttccggca cggcgatgcg gtgcaggccc gcctgctcca
     4741 tctcgcgcac gtgcttgacc gtgatgcgct tgtccttggc gacgatcggt ttgccgccct
     4801 tggtcgtgat gtcgaagcgc gcgatctccc cgcgcatgcg ctcgggcacg agctcgaact
     4861 ccacgccgtc cttgccgatc tggaaggtgt cgaacgcgaa gaattcggca aggatcttct
     4921 ccggcgtgta gcccagggcc ttgagcagga tcgtcaccgg catcttgcgg cggcgatcca
     4981 cccggaaata gaggtaatcc ttgggatcgt actcgaagtc gagccaggaa ccgcggtagg
     5041 ggatgacgcg cgccgagaac aggagcttcc ccgaggagtg cgtcttgccc cggtcgtgct
     5101 cgaagaacac gcccggagac cggtgcagct gcgacacgat cacgcgctcg gtgccgttga
     5161 tcacgaacga cccggtgctc gtcatcaggg gtatctcacc catgtagact tcctgctcct
     5221 tgcgctcctt gaccgggtgc ctgtcgcgac cgtctttctg cttgaccttg ggtgcttcct
     5281 tgtcgcggat caccagtccc accttggcgc gcaggggcgc ggcgtaggta agcccgcgct
     5341 gctggcactc cttcacgtcg aacggaggct cgcccagcgc gtaactgacg aagtcaagct
     5401 cggccactcc cgagtggctc tcgatcggga agatgctctt gaacgccgcc tgcaagccct
     5461 cggtcttacg gctttccggc acgacgttcg cctgcaggaa cgccgcatac gagtcgagct
     5521 gagtcgccag caagaacggc accggcaaca cgacgtcgcg cttggcgaag ctcttgcgga
     5581 tgcgtttctt ctcggtgaag gagtaagcca tatcagtatc agctccagga aaacatcaat
     5641 ccatgaacga taacggccac aatgcgcccc gcggcgaaca ccgcctggag tgcagggaaa
     5701 gttgcggccc gttcaaaaaa ggatccgggc tggcggactt tccgccagcc gagtctccac
     5761 atcgcgatta cttgatttcg ccggtcgcgc cggcttcggt gagttgcttg agtatgctct
     5821 ccgcgtcggc cttggaaact gcttccttga ccggcttggg agcgccgtcc accaggtcct
     5881 tggcttcctt gaggccgagc ccggtcacgg cgcgcaccac cttgatgacg ttgaccttgt
     5941 tggcccccgc gctattgagc atcaccgtga actcggtttg ctcttcggcc gcggcggctg
     6001 cggcgccgcc ggctgccggc gccgccaccg ccaccgtcgc tgcggcggcg gaaacgccga
     6061 acttgtcttc catctccttg atgagctggg agagctccag caccgtcatg tttgcgatgg
     6121 catcgagtat ttctgctttg gcaatagcca tgttttgctt gctcctgtca gatctatagg
     6181 ttgcgatatc cgaaatcccg gttctagcag gcttcaagcc gcctgcctct gtttctggtc
     6241 cctgaccgca gccagcgtcc gcgcgaactt gcccggcacc tcgttcaagg tgcggacgaa
     6301 cttcgcgact ggcgcctgca tcgtcgccac gagcttggcg agcagttccc ggcggctcgg
     6361 catgcgcgcg agttccgcca cttctctggc cgtcatcagc acgttgggca tggcgccggc
     6421 cctgatcacg aacttctcgt tgtccttggc gaactcctgc agcaccttgg cgacggccac
     6481 tggatcgctc gagatgccgt aagcgagggg accgaccatc ttgtcggtca ggcccttgaa
     6541 cggcgtatcg gcgaccgcgc ggcgcgccag ggtgttcttc agcacccgca gatagacgcc
     6601 cgagccgcgc gccttcttgc gcagttcggt catctccttc accgggatgc tgcggtactc
     6661 ggccagtatc acggcctggg ccttggcgag ctgcgaactc acctcggcca ccaccgcttg
     6721 cttctgctcc agatttagac ccaaggtcta tctcctttca cgaacacggc gaccggtgat
     6781 ccaggagtca gacacaaaaa cctgtttcgg gtgcgccgtc tgcgtaggac attcgggaat
     6841 gaggatcgag aatcgaggat tgaacaaccg ccctggggca gcatttgacc cttgaacccc
     6901 tgatccgttc ccaaaaatta aacctctcgg ttcctacggt ctttgacaac ccgccggcgg
     6961 tgcgcctcca gcggcccaaa gttccgcccc ggagttcgct ccggggtcca caatccatca
     7021 ctgctgcaag ctcgcctggt ccacgcgcac gcccacgccc atcgtgctcg acacggaaac
     7081 cttcttcaga tagatcccct tgacgctcga gggcttggac ttgttgaccg cgcccaccag
     7141 cgttttcaga ttctcggcaa gcgcgtcgac cgtaaacgac gcgcggccga tcgtgcattg
     7201 aacgatgccc gtcttgtccg cccggtactg gacctggccg gccttggcat tccggatcgc
     7261 cgttgccacg tcctgcgtga ccgtacccac cttggggttc ggcatgagcc cccgcgggcc
     7321 cagaatttgg ccaagcggcc ccaccacgcg catggcgtcg ggcgaggcga tcaccacgtc
     7381 gaaatccatc ttgccgccct tgatgtcgtt cgcgaggtcc tcgaacccga cgatgtcggc
     7441 accggcgtcc ctcgcctgtt tcgccttgtc gccctgggcg aacaccgcta tccgcacgct
     7501 cttgccggtg ccctgcggca acaccaccga gccgcgcacc gtctggtcgg acttcgaggc
     7561 atcgatgccg aggttgatgg agacatccac cgattcatcg aatttggcgg tggcgccgtc
     7621 cttcacgagc ttcaatgcct cctgcaccgg gtaggacttg gcgcgatcca ctttggcgcg
     7681 catcgcgttg tagcgcttgg aagccattta caggccctcc acgtccacgc ccatgctgcg
     7741 cgcgctgccg gcgatggtgc gcaccgccgc atcgaggtcc gccgccgtga gatcgggcag
     7801 tttcgccttg gcgatttcct cggcctgcgc gcgggtgatt ttgcccactt tctcggtatt
     7861 gggacgggaa ctgcccttct cgatcttggc cgccttccgg atcagcacgc tcgctggcgg
     7921 cgtcttcatc acgaaggtga agctcttgtc ctggtacgcg gtgatgacca ccggcaccgg
     7981 cagtcccggc tccatcttct gcgtctgggc gttgaacgcc ttgcagaatt ccatgatgtt
     8041 gagcccgcgc tggccgagcg ccgggccgat cggcgggctg gggtttgcct tgcccgccgg
     8101 cacctgcagc ttgatgatgc cgaccacttt ctttgccatc gcttctcctt acgggtgcca
     8161 gcggacgctc aaggcgtcct ccccatttaa catccgtgac tcgtgactcg tgactggtga
     8221 ttcgtatgca tccgcctgct cacgccttct ctacttggct gaaatcgagc tccaccggag
     8281 tggagcgccc gaagatcgtc accgacacgc gcagcttgtt cttgtcgtaa ttgacgtcct
     8341 cgacgttgcc gtggaagtcc gcgaaggggc cttccttgac ccgcaccgcc tcgccgacct
     8401 cgaacaggac cttcgggcgt ggtttctcca cgccctcctg gatctggtgc aggatgttct
     8461 gcacctcttt ctccgagatc ggggtcggct tggtcgcggt gccgccgacg aagccggtca
     8521 ccttcggcgt gttcttcacc aggtgccagg tatcgtcggt catctccatc tcgaccagca
     8581 cgtagcccgg gaagaacttc cgctcgctga tcgccttctg gccgctcttc atctccacca
     8641 cttcctcgac cggcaccagg atctgcccga acttgtcctg catgccggag cgcagaatgc
     8701 ggtcctgcag tgtgcgctgc acgctcttct cgaagcccga ataggcgtgc acgacatacc
     8761 atttcttgct catcatccac tctggcctaa cagctttttc acaatccata gcaggctcgc
     8821 gtcgaccgcc caaaggaaca ccgccatcac gaccacgaaa gcgaacacga cgcccgtggt
     8881 ctgcaacgtt tccttgcgcg tcggccacac caccttcttc gtttccgtca ccgactcctg
     8941 ggcaaacccg tggaactcct tgcccggcgc cgacatccag aacgtcaccg cgccggcaac
     9001 gaggcccgcc agcaccgcga cgacgcggag gatcatcggg ctctggtcca ggaaataaaa
     9061 tccgcccacg cccgccgcga ccagcaacag ggctgcagcg attttgatct tgtccgccat
     9121 atttcctttg gagtattgag tgttgagttt tgaatggtga gtcaacaaca aaaactcaaa
     9181 actaaacact taacattcat cactcgcgta gcgtttggca ggccaggagg gtctcgaacc
     9241 cccaaccttc ggttttggag accgacgctc tgccaattga gctactggcc tttgaattcc
     9301 gtgaatcgtg attcgactcg cgcttcacgc ccttattcaa tcaccttcgc caccacgccg
     9361 gcgccgaccg tcttgccgcc ctcgcggatc gcaaagcgca gcccctcttc catcgcaatg
     9421 ggctgaatca gggacaccgt gatcgatacg ttgtccccag gcatcaccat ctcggtgccc
     9481 gccgccagtt cgatggaacc cgtcacatcc gtcgtgcgaa aatagaactg cggccgatac
     9541 ccattgaaaa acggcgtgtg ccgcccgccc tcttccttgg agagcacgta gacctccgcg
     9601 gtaaacttcg tgtgcggcgt gatc
//
LOCUS       DBNGWZ010000170         4022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2787719, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000170 DBNGWZ010000000
VERSION     DBNGWZ010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2787719"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..208)
                     /locus_tag="AC16CX_05080"
     CDS             complement(5..208)
                     /locus_tag="AC16CX_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011918.1"
                     /translation="MKLREILKSPVFPLGKKWEFKKRTEGYESDVTALVRRMLEDEKI
                     QEDQRWAWERWRNDPDALKNRES"
     gene            complement(261..635)
                     /locus_tag="AC16CX_05085"
     CDS             complement(261..635)
                     /locus_tag="AC16CX_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011919.1"
                     /translation="MRKPARTLTALLLCLALPGCYVKLYGHQSTSGGTTTTTTASQVS
                     GSAKFDGGKVSFTSGQVPQPNAPGGHVYLGKGASAALVVGLVIADLVNYIRGEPAPVQ
                     RADGARIADTCTCYKKEVTSDK"
     gene            complement(673..1809)
                     /locus_tag="AC16CX_05090"
     CDS             complement(673..1809)
                     /locus_tag="AC16CX_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011920.1"
                     /translation="MNRRQFFILLIALAVLGGAGLALVWQDIAAYRASGAKIGAKLLP
                     GLKIADIAEVRLQDAKHQVTLARKEKAWVVQERGGYPASFQEISDLMIKLVELKVTQS
                     EQVGASLLPRVELAEPGKGEGSGTLVEFKDGAGKLLAGLIVGKKVLKKDPLNPLPAAK
                     DGVPAGRYVRVAGGKDTVVVVSDPLNAVEANPGRWLNKDFFKAERIRTLSVGPESGAP
                     RWKIARSEEWGQWRLAGGGNLDASAAVGAVNKLGSLVFNDVAAGPKAEVTEKPLVAVA
                     ETFDNLVYTVKLAKKAKSDDYLVSFTVAGEPPASRVPEKGEKPEDKERRDKDFAETRK
                     RLAERITAEQALAKWTYIVAKSEVEPLLKNRGDMLARKREDRRK"
     gene            complement(1806..3713)
                     /locus_tag="AC16CX_05095"
     CDS             complement(1806..3713)
                     /locus_tag="AC16CX_05095"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3011921.1"
                     /translation="MAKKPLETVLYSTGGVIALLAVLIAANFIVSALNLRADLTQGHV
                     YTLSPGTRKVLAKLEAPVKLRLYYTQGGEAVPVGLKTFAKRVEDLLNEYKAAAGGKVV
                     VEKFNPEPDSDAEDSAQLDGIEGQLTNTGEKFYLGLSISFLDQKAAIPVLAPDREQLL
                     EYDITRGIAQVAETKKSIVGVLSALPVMGRSLDPIRKQQPTEAWVLIQELKRVFELRE
                     VKLDAKKIDDDIKVLLVIHPRNITDETEYAIDQFVLRGGKLIAFVDPYAYFDQQPDFQ
                     NPFGGSQAGQSTLYNLFKAWGVDVDMGKVIADLTFASGAGPRLLPTLLSLNTQALNLD
                     DVVTSQVGTLLIPFGGAFKNKLAEGLKQTVLAHTSKNAMPVDLIIATLSGEPSTRGFQ
                     PTNQEMPLAIRLTGKFHTAFPEGGPKPFAPPRADKKKGAEEKKPEAKAEPQLKQSKEE
                     NSMVLVADVDLLSDGAAVEIQDVFGQRVAVPRNGNLALALGLVEHFSGDDALIGLRSR
                     ASFSKPLTVLREMEARAQQQYLGKIKELEDSLNQTQENLQKLQKSKAGATSSILTPEQ
                     QVELENFRKKAVETRRDLKELRKNLRVETDALEFWTKVVNIGLVPLLVALAGLGLALA
                     KRRRLKLQTAT"
     gene            complement(3718..>4022)
                     /locus_tag="AC16CX_05100"
     CDS             complement(3718..>4022)
                     /locus_tag="AC16CX_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011922.1"
                     /translation="AYLAVSCMTSAMTRNQVISFIVSVLICLFLILAGYTPVTDLLAR
                     FANPRVVEVVAAFSVMTHFEGFQRGVLDLRDLVFFASVMGFALFTTGVIIRNHRAG"
BASE COUNT          623 a         1344 c         1251 g          804 t
ORIGIN      
        1 cgaatcacga ctcacggttc ttgagcgcgt cagggtcatt gcgccagcgc tcccatgccc
       61 agcgctggtc ttcctggatc ttttcatcct ccagcatgcg ccgcaccagc gcggtgacgt
      121 cggactcgta gccctccgtg cgcttcttga actcccactt cttgccgagc gggaaaacgg
      181 gcgatttcag gatttccctc aacttcataa aggcgtgata ggtgataagt gtttacttcc
      241 ggcgctgccg gctgctcata tcacttatca cttgtcacct ctttcttgta gcaggtgcac
      301 gtgtcggcaa tgcgcgcgcc gtccgcgcgc tgcacgggcg ccggttcgcc gcggatgtaa
      361 ttcaccaggt cggcgatcac aagaccgacc acgagcgccg ccgaagcgcc tttccccagg
      421 tacacgtggc ccccgggtgc gttgggctgc ggaacctgtc ccgaggtgaa cgataccttg
      481 ccgccgtcga atttggccga gccactgacc tggcttgcgg tggtcgtcgt ggtcgtgccg
      541 ccgctggtgg attggtgccc gtagagtttc acgtagcagc caggcagcgc caagcacagc
      601 agcagggcag tcagcgtgcg cgctggtttc ctcatggcga acctagtata gcgccgcccg
      661 gacgaaaacg cgctatttgc ggcggtcttc ccgcttcctg gcaagcatgt cgccgcggtt
      721 tttcagcagc ggctcgacct cgctcttcgc gacgatatag gtccacttcg cgagcgcctg
      781 ctccgcggtg atgcgttccg cgagcctctt ccgggtttcg gcgaagtcct tgtcgcgccg
      841 ctccttgtct tccggcttct cgcccttctc cggcacgcgg ctcgccggcg gctcgcccgc
      901 caccgtgaag ctgacgaggt aatcgtcgct tttcgccttc ttcgcgagtt tcaccgtgta
      961 aacgaggttg tcgaaggttt ccgccacggc aacgagcggc ttttcggtta cttccgcctt
     1021 cggaccggct gcgacgtcat tgaagacgag ggagccaagc ttgttcactg ccccgaccgc
     1081 ggcgctcgca tcgagatttc cgcctccggc aagcctccac tggccccact cctcgctgcg
     1141 cgcgatcttc cagcggggcg caccgctctc cgggccgacc gacagcgtcc ggatgcgctc
     1201 cgccttgaag aaatccttgt tgagccatct gccggggttc gcttccactg cgttcagcgg
     1261 gtccgagacg accaccaccg tgtccttgcc gccggcgacc cgcacgtagc ggcccgccgg
     1321 gacgccgtcc ttggcagccg gcagcggatt caggggatcc ttcttcagca ctttcttgcc
     1381 gacgatcagg cccgccagca gcttgcccgc gccatccttg aactcgacca gtgttccgct
     1441 gccttcgcct ttccccggct cggccagttc gacccgcggc aggagggagg cgccgacctg
     1501 ctcggactgc gtcaccttga gctcgaccag cttgatcatg aggtcgctga tctcctggaa
     1561 gctggcggga tagccgccgc gctcctgcac tacccacgcc ttttccttgc gcgcgagcgt
     1621 gacctggtgc ttcgcgtcct gcaaccgcac ttcggcgata tccgcgatct tgaggcccgg
     1681 caggagcttc gcgccgatct tcgcgccgct cgcgcggtac gcggcgatat cctgccagac
     1741 cagcgccagc ccggcgcccc ccagcaccgc cagcgcgatg aggagaatga aaaactgcct
     1801 gcgattcatg ttgcagtctg cagcttcaga cggcggcgct tggcgagcgc gaggccgagc
     1861 ccggccagcg cgaccagcag cggcaccagt ccgatgttga cgaccttggt ccagaactcg
     1921 agcgcgtcgg tttcgacgcg caggttcttc ctgagctcct tgaggtcgcg gcgcgtctcg
     1981 accgccttct tgcggaaatt ctcaagctcg acctgctgct cgggcgtgag gatcgagctg
     2041 gtggcgccgg ccttcgactt ctgcagcttt tgcaggttct cctgcgtctg gttcaggctg
     2101 tcctcgagct ccttgatctt gcccagatac tgctgctgcg cccgggcctc catctcgcgg
     2161 agcaccgtga gcggcttgct gaaggaggcg cggcttctca ggccgatcag cgcgtcgtcg
     2221 ccggaaaaat gctcgaccag gccgagcgcg agcgcgaggt tgccgttgcg cgggacggcg
     2281 acgcgctggc cgaacacgtc ctggatctcg accgccgcgc cgtcggagag caggtccacg
     2341 tccgccacca gcaccatgga gttctcctcc ttcgactgct tcaactgcgg ctcggccttg
     2401 gcctctggct tcttctcctc cgcgcccttc ttcttgtccg cccggggcgg ggcgaagggc
     2461 ttgggcccgc cttccgggaa agccgtgtgg aacttgccgg tgagccggat cgccagtggc
     2521 atttcctggt tcgtgggctg gaagccacgc gtcgagggct cgcccgagag cgttgcgatg
     2581 atgagatcca ccggcatcgc gttcttcgag gtatgcgcaa gcacggtctg cttgagccct
     2641 tccgcgagct tgttcttgaa cgcgccgccg aacgggatga gcagcgtgcc gacctggctc
     2701 gtcaccacgt cgtcgaggtt caacgcctgc gtgttgagcg agagcagcgt cggcagcagc
     2761 cgcggcccgg cgccgctcgc gaaagtgagg tccgcgatca ccttgcccat gtccacgtcc
     2821 acgccccacg ccttgaacag gttgtagagc gtggactggc cggcctgcga gccgccgaac
     2881 gggttctgga agtcgggctg ctggtcgaag taggcgtagg ggtcgacgaa ggcgatcaat
     2941 ttcccgccgc gcagcacgaa ctggtcgatt gcgtattccg tttcatccgt gatgttgcgc
     3001 ggatggatca ccagcaacac cttgatgtcg tcgtcgatct ttttcgcgtc gagcttgact
     3061 tccctcaact cgaagacgcg cttcagctcc tggatcagca cccaggcttc cgtcggctgc
     3121 tgcttcctga tcggatcgag cgagcgcccc attaccggca gcgcgctcaa cacgccgacg
     3181 atcgacttct tcgtttcggc cacctgcgcg ataccccggg tgatgtcgta ttcgagaagc
     3241 tgttcgcgat cgggcgcgag cacggggatc gccgccttct ggtcgaggaa ggagatcgaa
     3301 agtccgaggt agaacttctc tcccgtgttg gtgagctgac cttcgattcc gtcgagctgc
     3361 gcggaatcct ccgcgtcgga atccggctcg gggttgaact tctcgaccac caccttgccg
     3421 ccggcggccg ccttgtattc gttgagcaga tcctccaccc gcttggcgaa ggtcttgagc
     3481 cccaccggaa ccgcctcgcc gccctgcgtg tagtagaggc ggagcttgac cggcgcctcg
     3541 agcttggcga ggaccttgcg cgtgccgggc gagagcgtgt agacgtggcc ctgggtgaga
     3601 tcggcgcgca ggttcaatgc gctcacgatg aaattcgccg cgatcagcac cgcgagcagc
     3661 gcgatgacgc cgccggttga gtagagcacc gtttccaatg gtttttttgc catggctcta
     3721 tcccgcccgg tggttgcgga tgatgacgcc ggtggtgaag agcgcgaacc ccatcaccga
     3781 ggcgaagaag acgaggtcgc gcaggtcgag aacgccgcgc tggaagccct cgaagtgggt
     3841 catcaccgag aacgcggcga cgacctccac cacccgcggg ttcgcgaaac gcgcgagcag
     3901 gtcggtcacc ggcgtatagc cggccagtat caggaacagg cagatcagca ccgacacgat
     3961 gaagctgatc acctggttgc gcgtcatggc ggaagtcatg cagctcaccg cgaggtatgc
     4021 gc
//
LOCUS       DBNGWZ010000171         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2284571, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000171 DBNGWZ010000000
VERSION     DBNGWZ010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2284571"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="AC16CX_05105"
     CDS             <1..1086
                     /locus_tag="AC16CX_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR3011923.1"
                     /translation="GSPVLLDFGSARQLAGGDQELTALVSPGYAPLEQYHTHGKQGPW
                     SDLYAIGGVMYWIVTGSKPVEAAARIRQDILPPAKQTGNSQLYGSELLAAIDWALKTH
                     EDERPQSVAGFRHALLGGQAVAAAVAPTVRLDAASVPTQIVEQQPITGVVFARGLLER
                     IETDLAKRIGPIASAVVMLAAAKAHDETELHHLLVNEIENLARRAREEADAKAESVRK
                     ARVEAEEAARARAAAEAAAEARLKVSEEAERKAREEAEARAEVESKAREEAEARTDTR
                     IRELQERARRALEEAEAKAEAVRKAREEVEEAARARAAAEAIVEAHSRAREEAELKAL
                     KEGEEAAQAQAKAEAMAKAKERDKHEK"
     gene            complement(1229..>2023)
                     /locus_tag="AC16CX_05110"
     CDS             complement(1229..>2023)
                     /locus_tag="AC16CX_05110"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3011924.1"
                     /translation="YLESLASALTGWNDPEARVARALKQDEFLLFGQSAVPLDQSRGL
                     PPYTEVLIRLQEEERHLTPPGAFLPIFEYLEMMPALDRWVIEHAAKWWRARKDARDVV
                     LGINLSGDTLGEAGFPAFVERLAKEHGLPPQSLCFELVGAEVAVAPLPAERAAKTLNS
                     LGCCFAITSFGRDLISFDALRTVGAALVKIDGAIVKEMHRDAVALAKVRSIQQVCGKT
                     GIRTVAEFVEQPETLNLLKEIGVDYAQGYGVARPAPLDGVPRAGDE"
BASE COUNT          395 a          660 c          705 g          263 t
ORIGIN      
        1 ggcagcccgg tgctgctcga cttcggctcc gcgcgccagc tcgcgggcgg cgaccaggaa
       61 ttgaccgcgc tggtgtcgcc cggctatgcc ccgctcgagc aataccatac ccacggtaaa
      121 cagggtccgt ggagcgacct ctacgccatt ggaggcgtga tgtactggat agtgacaggc
      181 agcaagccgg tggaagcggc ggcgcgcatc cgccaggata tcctgccgcc cgcaaagcag
      241 acgggaaact cccagctcta cggctcggaa ctgctggcgg cgatcgactg ggcgttgaag
      301 acccacgaag acgagcggcc gcagagcgtc gccggcttcc gccacgctct gcttggcggt
      361 caggcagtgg ctgcagcggt cgcgcccacc gtccgtctgg atgccgcctc cgtgccgacg
      421 caaattgtcg aacagcagcc gataaccggc gtcgttttcg cccgcgggct gctggaacgc
      481 atcgaaaccg atctcgcgaa acgcatcggc ccgatcgcgt cagcggtggt gatgctcgcc
      541 gccgccaaag cgcacgacga aaccgagctt caccacctac tcgtcaacga gatcgagaac
      601 ctggcgcggc gcgcgcgcga agaagccgac gccaaggccg aatcagttcg caaggcgcgc
      661 gtggaagccg aagaagccgc gcgggcgcgg gcggcggcag aggcggcagc cgaggccagg
      721 ctcaaggtaa gcgaggaagc tgaacgcaag gcgcgcgaag aagccgaggc cagggccgag
      781 gtcgagagca aggcacgcga agaagcggaa gcgcggaccg acacccggat cagggaactg
      841 caggagcggg cgcggcgcgc actcgaggaa gccgaggcca aggccgaagc cgttcgcaag
      901 gcgcgcgagg aggtggagga agccgcgcgg gcgcgggcgg cggccgaggc gatagtcgag
      961 gcccatagca gagcgcggga ggaagccgag ctcaaggcgc tcaaagaggg cgaggaagcc
     1021 gcgcaggctc aggcgaaggc cgaagcgatg gccaaagcca aggaacgcga caagcatgaa
     1081 aaataatggt cgcgtgcggc gttcgctggc gactgatcat ttccggaaag cgtaacgcgt
     1141 aatgcatgat gatgaggctt gaagtgaagc gtgactggca agccgcccac gccctcggca
     1201 cccgtcactt cgtcactgcg tcacttcgtc actcgtcacc ggctcgggga acgccgtcga
     1261 gcggcgcggg ccgcgccacg ccgtagccct gggcgtaatc cacgccgatt tccttcagca
     1321 ggttcagcgt ctccggctgc tcgacgaact cggccaccgt gcggatgccg gtcttgccgc
     1381 aaacctgctg gatcgagcgc accttggcga gcgccacggc gtcgcggtgc atctctttca
     1441 cgatcgcgcc gtcgatcttg acgagggcgg ccccgaccgt cctcagcgcg tcgaacgaga
     1501 tcaaatcgcg cccgaacgag gtgatggcga agcagcagcc gagcgagttg agtgttttcg
     1561 ccgcgcgctc ggccggcagc ggcgcgacgg cgacctcggc cccgacgagc tcgaagcaca
     1621 gggattgcgg cggcagcccg tgctccttcg ccagccgctc gacgaaagcg ggaaaaccag
     1681 cctcgcccag cgtgtccccg gagagattga tgccgagcac gacatcgcgg gcgtccttcc
     1741 tcgcgcgcca ccatttcgcg gcgtgctcga tcacccagcg gtcgagcgcg ggcatcattt
     1801 ccaggtactc gaagatcggc aggaaagcgc ccggcggcgt caggtggcgt tcctcctcct
     1861 ggaggcggat cagcacctcg gtgtacgggg gcaacccgcg cgactgatcc aagggcacgg
     1921 cggactggcc gaacagcagg aactcgtcct gcttcagggc gcgggcgacg cgggcctcgg
     1981 gatcgttcca tcccgtcagc gccgaagcga gcgactccag ata
//
LOCUS       DBNGWZ010000172         9620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_646778, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000172 DBNGWZ010000000
VERSION     DBNGWZ010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_646778"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16CX_05115"
     CDS             complement(<1..611)
                     /locus_tag="AC16CX_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3011925.1"
                     /translation="MSAAPDDPRVVRGMSAELAHWRRRLASGEAPLGWKVGFGAPAAM
                     EKLSIKAPLVGFLTDRALLPSGATVSLSGWTRPAAEAEIAVHMGRDLPGGASRAKTRQ
                     AIAGLGPAIEIVDVDSPPDDVERILAANIYQRHVILGPCDASRAGCVLEGLTCRIARG
                     GSAFAQTADPQAMTGELVGIVHHVAELLAACGERLRGGQVIITG"
     gene            complement(628..1059)
                     /locus_tag="AC16CX_05120"
     CDS             complement(628..1059)
                     /locus_tag="AC16CX_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3011926.1"
                     /translation="MKPDAHTVLKPYHTNLRDVPKVGGLKAKDGWINMQVQFLIGQKT
                     CNSDRLLVGWTVLPPGAQHDRHRHFNCDEFWIVIKGRGVMYTEDGERPSEEGDVVFTP
                     RGHWHGFKNTSSEEVVLVWGWSGAGSLEAAGYESVEGGRTH"
     gene            complement(1056..1940)
                     /locus_tag="AC16CX_05125"
     CDS             complement(1056..1940)
                     /locus_tag="AC16CX_05125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3011927.1"
                     /translation="MQIGFIGLGAMGAGIVPRLLAAGHAVTGWNRTRSKAELLVRAGM
                     SWADSPRAVAARSEVVFSILTDAAAVRSVALGESGVVAGLKLGGMFVDMSTIAPDASR
                     AIAAEVARAGFVMLDAPISGSPVTLAQGKASIMVGGDRAAFERIRPVLLAIGQRATHI
                     GANGLAAQMKLAINLVLMVEVIAFGEAVALAEKGGVSRDVAVDAMLNSVVASPVLGYR
                     GPFILDGQMPEVPLADVNLQQKDMTLVLELARRIGAPVPLAAAANEMMNACRGMGLDH
                     HDFVVAHEAYLRLGGATR"
     gene            complement(2066..3256)
                     /locus_tag="AC16CX_05130"
     CDS             complement(2066..3256)
                     /locus_tag="AC16CX_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3011928.1"
                     /translation="MSYPLEGVRVLELGQIIAGTYGSQVLSDLGAEVIKIEAPEGDLG
                     RIPSVAPYKGVSTLFLTFNRNKKSVVINLKAEPGRALFYDLVKVSDVVIDNFRAGVLE
                     RLRIDYTTLSAINPRIIHCSVTVFGTEGAYKDYPALDLVIQAISGHLSITGEPGRPPA
                     RVGIPLGDLCGGIFSCKAVLAALFQRERTGRGCRIETSMFDGMLNLLSYMGTHYLNTG
                     EVPTPQGSAHEFTVPWQAFQAKDGYIVIATRQENFWRKLCAVLGEPGLAEDPRFATNP
                     KRLENRAVLIPIIENIVRTRTVAEWLKQLRAAEVPAAPVNDLAGAFAEPPVAEREMIV
                     EYDHPDVGKVRLPGNPIKMSGVPGTPSRPAPRLGEHTDAVLSELLSLPAAKIAELRRQ
                     GAIS"
     gene            complement(3398..4540)
                     /locus_tag="AC16CX_05135"
     CDS             complement(3398..4540)
                     /locus_tag="AC16CX_05135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3011929.1"
                     /translation="MDFSFTPEQESLRGHVQELLRAVCSPEYAERCDREARPPREAYE
                     ALGRHGWFGLSIPPEYGGSGGSAIELAILLEETGRHFEELAMWVFRTLTYGGYAVLQH
                     GTPEQKRSLLPRIARGELSFCLGLTEPQAGSDAAALTTRAARAGDGYVINGQKVFTSG
                     MDISDYCLLITRTSTGEKKQQGITNFLVDTKLPGIEVRKIETLGQRAIGTTQVFYTDV
                     KVPASAVLGQVDRGWEAVDAYLWYERLCLSAARTGAASAAFDYALNYAKTRKQFGRAI
                     GEFQAISHKLADMKVMLDLSRMLVYRFAWQLAQGSATRHDAAVVKLYTGEAYKSISDM
                     GLQILGGYGYCMEYPMQRFFRDSRLAVIGAGTSEIQRNIIARGLGL"
     gene            complement(4543..5436)
                     /locus_tag="AC16CX_05140"
     CDS             complement(4543..5436)
                     /locus_tag="AC16CX_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3011930.1"
                     /translation="MKPVRVALFAPGSKERVMAKALESGAHAVILDLEDSVPIAAKAE
                     ARALVARVIDAAAALPAASRPVITVRTNSATTGLLPDDLSAVVRPGLDAIFLPKVESA
                     EEMTGTAALLDRLEAAKGLAAGAVRIIPMFESALGVLRCFELARATPRVAATCIGSAR
                     DGDLQTDLGCGWSIEGVELLYARSKILLDSRAAGVYALDGVFSDLNDEAGFIQDCTLS
                     ARLGYIGRTVIHPKQIAPARQAYAVSEADAAYYRRVVSEFEAVERTGAAAAITVDGKL
                     VDYAMYQRAKRVLEWARLDGR"
     gene            complement(5625..6422)
                     /locus_tag="AC16CX_05145"
     CDS             complement(5625..6422)
                     /locus_tag="AC16CX_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3011931.1"
                     /translation="MKIWWQSSTPIHRLHDYRTTLTEHLTSVKRPETEIHINGVDDGS
                     MDLHYNAVVAVNSYGPGGVLNKIIQAADQGYDAVAIGCFLDPALQEAREVVPIPVLGL
                     GEASMLTACMYGHRFSGVAFHSKQAQYYDRKAFEYGLASRHVPFGNLGIDFNEVQTAF
                     ASPERLTGTFLGETRRLATQGAEVILAACATVNAIIRREKIQEVDGALVMDCNAVLLK
                     LTEAMAELAASAGLRASQRLLYQRPDRRSLGEWMRIYNFRASPQPKP"
     gene            complement(6575..7300)
                     /locus_tag="AC16CX_05150"
     CDS             complement(6575..7300)
                     /locus_tag="AC16CX_05150"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR3011932.1"
                     /translation="MQSSWTPFSYIVVNLARQIRPGEIAFSGVNSTLPMLACLLAKRA
                     YDFHFTYLNVAGGVEPVPKKIPHSSSDPAIVEGSPAIFANEDFYDLCARGGLDLVFLG
                     CAQVDGEGRTNVSVIGDWHNPRVRLPGGGGAAVMMPTAKRVATWRTEHSTRTLVEKLD
                     FVTAAGNLQTVVTPIAVFQKRNGRLALESWNPQVTLDEVIRRTGFRFDAERAVPTAPI
                     GASEREALASLDPRGEFAAEVRA"
     gene            complement(7373..7768)
                     /locus_tag="AC16CX_05155"
     CDS             complement(7373..7768)
                     /locus_tag="AC16CX_05155"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR3011933.1"
                     /translation="MKDPYGSGQEVYVIPAIRPDVAVIHANEIDELGNARVLGSPFWD
                     HPLTRAAKRVLVTAERLVPTEVLSAQPELTLVPGFMVEATAVVPRGAWPGSMHPLYEV
                     DYPAVEAYLRDEPGALEAHLAAAPEQSEK"
     gene            complement(7883..8194)
                     /locus_tag="AC16CX_05160"
     CDS             complement(7883..8194)
                     /locus_tag="AC16CX_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3011934.1"
                     /translation="MAQSPSSKLCSLPELAARIPDDCSLGLGGVFLHRGPFALVRELA
                     RQGRRGLEIIKSSPGYDLDLLCRAKAVARVRAGIVAMEGSFGLAPWYRKVIEQKEARL
                     E"
     gene            8456..8959
                     /locus_tag="AC16CX_05165"
     CDS             8456..8959
                     /locus_tag="AC16CX_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3011935.1"
                     /translation="MGATESKLAKMGLRLPPVHPYPSPNRIPCVQVGNILFLGGHSTA
                     RHPLMLDAKNTGKVGSEITEQEAYVAARGVALQILASIKAQVGDLDRVKRVVRLHGMV
                     NSAPGFERQFAVIDGASDLFYELWGPERGAHARSAVGVAELPRRSILEIVGEFELYPE
                     RRAGRAR"
     gene            9086..>9620
                     /locus_tag="AC16CX_05170"
     CDS             9086..>9620
                     /locus_tag="AC16CX_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3011936.1"
                     /translation="MSRAKGVREVGYVDVRQGGGVRHAHAATGRRMRPASWLDCAGGG
                     QVVVEQGIAYIGTMRNPHGTLIVDVSDPKRPKQLASLSMPAGTHSHKVRVANSIMVTN
                     REVLPKHALHGEIPPAEFRGGLGIYDVSNPARPRLITSWEVTGGSGVHRFDFDGRYAY
                     ISPTADGYIGNIVMILDL"
BASE COUNT         1626 a         3188 c         3143 g         1663 t
ORIGIN      
        1 ccggtgatga tgacctggcc gccgcgcaac cgttccccgc acgccgccag cagctcggcg
       61 acgtgatgaa cgattccgac cagctcgccg gtcattgcct gcgggtccgc cgtttgcgcg
      121 aacgcgcttc cgccgcgcgc gatgcggcac gtgagtcctt ccagcacgca gcccgcgcgc
      181 gaggcgtcgc acgggccaag aatcacgtgg cgctggtaga tgttggcggc gaggatgcgc
      241 tcgacgtcgt caggcgggga atccacgtcg acgatctcga tggcgggtcc cagcccggcg
      301 atcgcctgcc gggtttttgc ccggctggcc ccgcccggca ggtctctgcc catgtggacg
      361 gcgatctcgg cttcggcggc gggcctggtc cagccgctca atgaaacggt agcgccggaa
      421 ggaagcagtg cgcggtcggt gagaaaaccg accagcggcg ccttgatcga gagcttttcc
      481 atcgccgcgg gcgcgccgaa accgactttc catccgagcg gcgcctcgcc gctcgcgagg
      541 cgccgtcgcc agtgggcgag ttcggccgac attccgcgca cgacacgcgg atcatccggg
      601 gccgcgctca tgacgggacg gcgagggtca gtgcgtccgc ccgccttcca cggattcata
      661 tccagccgct tccagcgagc ccgctccgct ccagccccac accagcacca cctcctcgct
      721 ggaggtgttc ttgaatccgt gccagtgtcc gcgcggcgta aacacgacgt cgccttcctc
      781 tgaaggccgt tcaccgtcct ccgtgtacat cacgccgcgg cccttgatga cgatccagaa
      841 ctcgtcgcag ttgaagtggc ggtgccggtc gtgctgggcg cccggcggca acacggtcca
      901 gcccaccagc agccggtccg agttgcaggt cttctggccg atcaggaact gcacctgcat
      961 attgatccag ccatccttcg ctttgaggcc gccgactttc ggcacgtccc tcagattggt
     1021 gtgatagggc ttcagcacgg tgtgcgcatc gggcttcatc tcgttgctcc tcccaggcgg
     1081 agataggctt cgtgggcaac cacgaaatca tggtggtcga gacccatacc gcggcatgcg
     1141 ttcatcattt cgttggcggc tgccgcgagc ggcaccggtg cgccgatcct gcgcgcaagc
     1201 tcgagcacca gcgtcatgtc cttctgctgc aggttgacgt cggcgagcgg cacttccggc
     1261 atctgcccat ccagtatgaa cgggccgcgg tagccgagca ccggcgaggc gacgacgctg
     1321 ttcaacatcg catccaccgc gacatcgcgg gagacgcctc ccttttccgc gagcgccacg
     1381 gcctcgccga aggcgatgac ctcgaccatc agcaccagat tgatcgccag cttcatttgc
     1441 gcggccaggc cgttcgcccc gatatgcgtc gcccgctgcc cgatggcgag caggaccggc
     1501 cggatgcgct cgaacgcggc gcgatcgccg cccaccatga tcgacgcctt gccctgcgcc
     1561 agcgttaccg ggctgccgga gatcggagcg tcgagcatca cgaaccccgc cctggccact
     1621 tcggcggcga tggcccggct cgcatccggt gcgatcgtgc tcatgtccac gaacatgccc
     1681 cccagcttga ggcctgcgac gacgccgctt tcgccgagcg cgacgcttct caccgcggcg
     1741 gcatcggtga ggatggagaa gaccacctcg gatcgggccg cgaccgcgcg tgggctgtcg
     1801 gcccacgaca ttcccgccct gaccaggagt tccgcctttg atcgcgtgcg gttccacccg
     1861 gtaacggcat ggcctgcggc cagcaggcgc ggaacgattc cggcgcccat cgcgcccagt
     1921 ccgatgaatc caatctgcat tgcgtatccc cctgacgcag gtcgaccggc ccccgccgcc
     1981 cgtccacctc ggcagatcgg cgtcgatcga gcctaccggc cggcgcgggg aaaatcaacc
     2041 ggcgcgcccc ggcgcgcgct cacccctaac tgatcgcgcc ctgtcgccgc agctcggcga
     2101 ttttcgccgc gggtagcgac aagagctcgc tcaggacggc atcggtatgc tcgcccagcc
     2161 gcggcgccgg gcgcgacggc gtgcccggga cgcccgacat cttgatcgga ttgccgggca
     2221 gccgcacctt accgacgtcc gggtggtcgt actccacgat catctcgcgc tcggcgaccg
     2281 gtggctcggc aaacgcgcca gccagatcgt tcacgggcgc ggccggcacc tcggccgcgc
     2341 gcagctgctt gagccattcg gcgacggtgc gcgtgcgcac gatgttctcg atgatcggta
     2401 tcagcactgc gcggttttcc agccgcttcg ggttggtcgc aaagcgcgga tcctcggcga
     2461 gtcccggctc gcccagcacc gcgcacaact tgcgccagaa attctcctgc cgggtcgcga
     2521 tcacgatata accgtccttg gcctggaacg cctgccacgg caccgtgaat tcgtgcgctg
     2581 aaccctgggg cgtcggcacc tcgcccgtgt tcagatagtg ggtgcccata tagctcagga
     2641 ggttcagcat gccgtcgaac atggaagtct cgatccggca tccccgcccg gtgcgctcgc
     2701 gctggaagag ggccgccagt accgccttgc acgagaaaat cccgccgcag agatcgccga
     2761 gcggaatgcc gacgcgcgcg ggcgggcgac cgggctcgcc ggtaatcgag agatgaccgc
     2821 tgatggcttg aatgacgaga tcgagggccg gatagtcctt gtaagcgcct tcggttccga
     2881 agacggtgac cgagcagtgg atgatgcgcg gattgattgc cgagagagtc gtgtaatcga
     2941 ttctcagacg ctccagcacc ccggcacgaa agttgtcgat caccacgtcg gagaccttca
     3001 ctagatcgta gaagagcgcc cgccccggct cggccttcaa gttgatgaca acgctcttct
     3061 tattgcggtt gaaggtgaga aacagcgtgc tgacgccctt atacggcgca accgagggga
     3121 tgcggccgag atccccttcg ggcgcctcga tcttgatcac ctcggcgccg aggtcggaga
     3181 gcacctggct gccgtaggtg ccggcgatga tctggccgag ttccagcacg cgcacgcctt
     3241 caagcggata ggacattgca acgatgaatg atgagtgatg agtgatgagc ggtggttgag
     3301 taggctttca ccctctgtgt tctcttggtt ctctgtggct ggagatcgga ctaacgcgtc
     3361 tctgtctcat gaaattccca gttcaggatt ggggctctca gagccccaat ccccgggcga
     3421 taatattgcg ctggatctcg gaagtgccgg cgccgatcac ggcgagccgc gagtcgcgga
     3481 agaagcgctg catcgggtat tccatgcaat agccgtagcc gccgaggatc tgcagcccca
     3541 tgtcggagat cgatttgtac gcttcgcccg tgtagagctt caccaccgcc gcgtcgtgac
     3601 gcgtagcgct tccctgcgcg agctgccacg cgaagcggta gaccagcatg cgcgagaggt
     3661 cgagcatcac cttcatgtcg gcgagtttgt gggagatcgc ctggaactcc ccgattgcgc
     3721 gcccgaactg cttgcgggtc ttggcgtagt tgagcgcgta gtcgaaggcc gcggatgccg
     3781 ccccggtgcg cgcggccgag aggcacaggc gctcgtacca gagataggcg tccactgctt
     3841 cccagccgcg gtcgacctgc ccgagaacgg ccgaggccgg caccttgacg tccgtataga
     3901 acacctgggt ggtgccgatc gcgcgctggc cgagcgtctc gatcttgcgc acctcgatgc
     3961 ccggcagctt ggtgtccacc aggaagttgg ttatgccctg ctgcttcttc tctccggtgg
     4021 aggtgcgggt gatgagcagg caatagtcgc tgatgtccat gcccgaggtg aacacctttt
     4081 gcccgttgat gacgtagccg tcgcccgctc gcgcggcgcg tgtggtgagc gcggcggcat
     4141 cggagccggc ctgtggttcc gtcagcccga ggcagaacga gagctcgccg cgcgcgatac
     4201 ggggcaggag agagcgcttc tgctcgggcg tgccgtgctg caacacggcg tagccgccgt
     4261 aggtgagcgt gcggaacacc cacatcgcga gctcctcgaa gtgccgcccc gtttcctcca
     4321 gcaggatcgc gagctcgatc gcggagccgc cgctgccgcc gtactcgggc ggtatgctga
     4381 ggccgaacca gccgtgcctg ccgagcgcct cgtacgcttc gcgcggcggg cgcgcctcgc
     4441 ggtcgcagcg ctcggcgtac tccggcgagc agaccgcgcg cagcagctcc tgcacgtgtc
     4501 cgcgcaggct ttcctgttcc ggcgtgaagg agaaatccac cctcacctcc cgtcaaggcg
     4561 cgcccattcc agcacgcgct tcgcgcgctg gtacatggcg taatcgacga gcttgccgtc
     4621 cacggtgatt gcagccgccg cgccggtcct ttccactgcc tcgaactcgc tcaccacgcg
     4681 ccggtaataa gcagcgtccg cttcggacac cgcgtacgcc tgccgcgcgg gcgcgatctg
     4741 cttggggtgg atcaccgtgc gcccgatata acccaggcgc gcggagagcg tgcagtcctg
     4801 gatgaagccc gcctcgtcgt taaggtcgct gaagacgccg tcgagcgcgt agacgcccgc
     4861 ggcgcggctg tcgagcaaga tcttggagcg cgcgtagagg agctccacgc cctcgatcga
     4921 ccagccgcag ccgaggtcgg tctgaagatc gccgtcacgc gcgctgccga tgcaggtggc
     4981 ggcgacgcgc ggcgtggcgc gcgcgagctc gaagcagcgc aacacgccga gcgcgctctc
     5041 gaacatgggg atgatgcgca ccgcacccgc ggcgaggccc ttcgcggcct ccaggcggtc
     5101 gaggagggcc gccgtgcccg tcatttcctc ggcggactcc accttgggca ggaagattgc
     5161 gtccagcccc gggcgcacca ccgccgagag atcgtcgggg agcagcccgg tcgtggcgct
     5221 gttggtccgc accgttatta cgggacgtga ggcggcgggc agcgcggcgg cggcgtcaat
     5281 caccctggcc accagcgcgc gcgcttctgc cttggccgcg atcggtaccg aatcctcgag
     5341 gtccagaatc acggcgtggg cgccgctctc gagcgccttc gccatcacgc gctccttgct
     5401 gccgggcgcg aacagggcga cgcgtacggg cttcaaatcg tgcctcctcc ggacgggtgc
     5461 tgccggaatg ataacagcca gcccgcatga ttcatgattg tcacggttcc gggggtcgcg
     5521 cgatcgaagc gcacgtggac aggtgcgaca tgcgggctga attgtttttc cggcaattta
     5581 atttcaattt tcaggaattt gtcggaccgc aatccgataa attcctaggg cttcggctgc
     5641 gggctcgcgc gaaaattgta gatccgcatc cactcgccca gcgaacggcg atccgggcgc
     5701 tggtagagca gccgctggct cgcgcgcagt ccggcgctcg ccgccagttc cgccatcgcc
     5761 tccgtcagct tcagcaatac ggcattgcag tccatgacga gggcgccgtc cacctcctga
     5821 attttctccc tgcggatgat ggcgttcacc gtcgcgcagg cggcgaggat cacctccgcg
     5881 ccttgcgtcg cgagccggcg cgtctcgccg aggaaggtcc cggtcagccg ctcggggctc
     5941 gcaaaggcgg tctgcacttc gttgaaatcg atgccgagat ttccgaacgg cacgtgccgc
     6001 gaggcgaggc cgtactcgaa cgccttgcgg tcgtagtact gcgcctgctt ggagtgaaat
     6061 gcgacacccg aaaacctgtg cccgtacatg caggccgtca acatggaggc ctcgcccagg
     6121 cccaggactg gaatcggcac cacctcgcgc gcctcctgca gcgccgggtc gagaaagcag
     6181 ccaatcgcca ccgcatcgta gccctggtcc gccgcctgga tgatcttgtt cagcacgccg
     6241 ccgggcccat agctgttgac ggcgacgacg gcgttgtaat ggagatccat cgagccgtca
     6301 tccacgccgt tgatgtggat ctcggtctcc ggccgcttca cgctcgtcag gtgctcggtg
     6361 agcgtggtac ggtaatcatg caggcgatgg atcggggtgc tgctctgcca ccagatcttc
     6421 atgtcgggct cctccaaagc tccgcccggc gacgttcagg aaagtgggca tgggcggcac
     6481 ggcgttatgc tacgtgcggc ctcgcggatc tctttgatcc atatcaagag ttgacgaata
     6541 aggagcttgc cggaccaggg tcaggaaaac tccttcaggc gcgcacttcg gcggcgaatt
     6601 caccccgcgg gtcgaggctc gcgagggctt cgcgctcgct cgctccgatc ggagcggtgg
     6661 gaacggcccg ctccgcgtcg aagcggaaac cggtgcgccg gatgacttca tcgagcgtca
     6721 cctgcgggtt ccaggattcg agcgcaaggc gcccgttccg tttctggaac accgcgattg
     6781 gcgtcaccac cgtctgaagg ttgccggctg cggtgacgaa atcgagcttc tccaccagcg
     6841 tgcgcgtgga gtgctcggtg cgccaggtcg caacccgctt cgcggtcggc atcatcaccg
     6901 ccgcgccgcc gccgccgggc agccgcaccc tggggttgtg ccagtcgccg attaccgaaa
     6961 cgttcgtgcg cccctcgccg tccacctgcg cgcagccgag aaacacgaga tcgaggcccc
     7021 cgcgcgcgca gaggtcatag aaatcctcgt tggcgaagat cgccggcgag ccttctacga
     7081 tggccgggtc gctcgacgag tgcgggatct tcttcggcac cggctcgacc ccgccggcga
     7141 cgttgaggta ggtgaaatgg aagtcgtagg cgcgcttggc aagcaggcac gcgagcatgg
     7201 gaagcgtgga attcaccccg ctgaacgcaa tctcgcccgg gcgtatctgc cgggcgagat
     7261 tgacgacgat gtaagagaac ggggtccagc tgctttgcat acgttgctcg tttctcgttc
     7321 ctcgtttctc gttgtttggc cttgcttcaa ttaaccggta acgagcaacg agtcactttt
     7381 cagattgttc cggcgcggcg gcgaggtgag cttccagcgc gccgggttcg tccctcaagt
     7441 aggcctcgac cgccgggtaa tccacctcgt agagcgggtg catcgagccc ggccaagcgc
     7501 cgcgcggcac gaccgcggtg gcttccacca tgaatcccgg cacgagcgtc agttccggct
     7561 gcgcgctcag cacttcagtc ggaacgaggc gctcagcggt gacgagcacc cgcttcgccg
     7621 cgcgcgtgag cggatggtcc cagaacggcg agccgagcac gcgagcgttg ccgagctcat
     7681 cgatctcgtt cgcgtggatc accgcgacgt cgggccggat cgcggggatg acgtagactt
     7741 cctgccccga cccgtaaggg tccttgatct attgccagcc gttcagcgcg gcgaggtcgc
     7801 tgccgtgaac gccacccacc ggctggaagg ggatcccgta tgcggctgca cgcagcccgg
     7861 cgacgagcgt cattcaggcg tgctattcga gccgcgcctc cttctgctcg atgaccttgc
     7921 ggtaccacgg agcgagcccg aagctcccct ccattgcgac gatgccggcg cgcacgcggg
     7981 caactgcctt ggcgcggcac agcaggtcga ggtcgtagcc gggggaagac ttgatgatct
     8041 ccagcccgcg ccgaccctgc cgcgcgagct cgcgcaccag cgcgaacggg ccgcggtgca
     8101 ggaagacgcc gcccaggccg agcgagcagt cgtcggggat gcgcgccgca agctcaggca
     8161 ggctgcacag cttggaggaa ggggactggg ccatctctcg ggacagctga agaatgagca
     8221 aaaataatag cactaaggaa ttcgtcgggc agtaacccga cgaattcctg atttccggtt
     8281 aaactccgca ctctcaagag ggagattcgt atgcacgctg aagtcaagga tcgcgccgcc
     8341 accaccggcg tttggagcgc ggaatacttg gcgaagatcc aagcctttct gacgatgcct
     8401 gagaaccgct cgatcggcgc ctgagacgcc atacgcagga accgaggggg aaaacatggg
     8461 cgcgaccgag agcaagctcg cgaaaatggg tctgaggctg ccgccggtcc atccctaccc
     8521 gagccccaac cgcataccct gcgtgcaggt cgggaacatc ctgtttctcg gcgggcacag
     8581 caccgcgcga catccgctca tgctggacgc gaaaaatacc ggcaaggtcg gttccgagat
     8641 caccgagcag gaagcgtacg tggccgcgcg cggggtggcg ctgcagattc tcgcatcgat
     8701 caaggcgcag gtcggcgacc tcgaccgcgt gaaacgcgtc gtccggctgc acggcatggt
     8761 gaactccgcc ccgggcttcg agcgccaatt cgctgtgatc gacggcgcct ccgatctgtt
     8821 ctacgagtta tggggcccgg aacgcggcgc ccatgcgcgc tccgcggtcg gcgtggcaga
     8881 gctcccgcgc cgctcgatcc tcgagatcgt gggtgaattc gagctctatc ccgagcgccg
     8941 tgccggaaga gccaggtgaa accgccgata aacgcagata aacacagata atttatagcc
     9001 aggatggccg tcggcaggga atttcgtccg cgtatatctg cgtttatctg cggttagtaa
     9061 ttgtttgtga cgttgggggt agggcatgag cagggcgaag ggcgtacgcg aagtcggcta
     9121 cgtagacgtg aggcagggcg gcggcgtacg acacgcgcat gccgcgaccg ggcggcgcat
     9181 gcggccggcg tcgtggctcg attgcgcggg cggcgggcag gtggtcgtgg agcagggcat
     9241 cgcctacatc ggcaccatgc gcaacccgca cggcacgctg atcgtggacg tctcggatcc
     9301 gaaacgcccg aagcagctgg cgtcgctttc gatgccggcg ggtacccatt cgcacaaggt
     9361 gcgggtggcg aacagtatca tggtgaccaa ccgcgaggta ctgccgaagc acgcgcttca
     9421 cggcgaaatc ccgccggcgg aatttcgagg gggactcggc atctacgatg tttccaatcc
     9481 cgccaggccc aggctcatca cgagctggga ggtgaccggc gggtcgggcg tccaccgctt
     9541 cgatttcgac gggcgctacg cctacatctc gcccaccgcg gacggctaca tcggcaacat
     9601 cgtgatgatt ctcgacctga
//
LOCUS       DBNGWZ010000173         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1780581, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000173 DBNGWZ010000000
VERSION     DBNGWZ010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1780581"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16CX_05175"
     CDS             <1..360
                     /locus_tag="AC16CX_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3011937.1"
                     /translation="AAVIHQRPSGYSFLPELADCACQQVSVETAQSNLDCSVLAKLAG
                     KKIMVGALDLSDMTVETPAVVVARIKRALPYVKPEDVIIAPDCGMKYLPRDVAFGKMR
                     AMVEAAKVLRDEYGGGP"
     gene            complement(417..1403)
                     /locus_tag="AC16CX_05180"
     CDS             complement(417..1403)
                     /locus_tag="AC16CX_05180"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3011938.1"
                     /translation="MNLRSRSIAVVLSLLALPLPAAHSAAQPQAGDFPNKPIRWIIPF
                     PPGGSNDMLGRYLGAKLTDRVGQQVVIDNRGGANGIIAAGLAANAPADGHTLLMVSTS
                     WVMNAVLRPLPYDVEKSFDPISMIGSSPNSIVVFPGLGVGSLRELVERARAKPGALQY
                     ASTGVGGFNHFGGELFKKVAGIDMVMVPYKGGGPAMIDVMAGNMPVMFTSVTQVLPHV
                     RAGRIKMLAVGAAKRNLAVPDIPTVAEAGFPGYEVYVWWGVAAPAGVPRAVLEKIRRE
                     ITAILREPETQKRLAGEAAEPRIITPVEFRKMVRDEKKKWADIARQAGIKVN"
     gene            1591..2007
                     /locus_tag="AC16CX_05185"
     CDS             1591..2007
                     /locus_tag="AC16CX_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011939.1"
                     /translation="MLLSLLIVTFVVALFTSWVVVRFFSAPVEAILKRIIDDEIRSAW
                     LKYLKFAIYVVGVSKGVRVLELERYVTPSRFDKDARILELTAERWLLEIYRTVIETLQ
                     GIAWMLLIFFIFALIAYVIVRVFELRRGSKPETGDG"
     gene            2086..2568
                     /locus_tag="AC16CX_05190"
     CDS             2086..2568
                     /locus_tag="AC16CX_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3011940.1"
                     /translation="MANNPSESKWKFHGVRVVHANELDINTPQTPGMNRAAAVTYARA
                     GAEKLWAGTVVIHPKAKTGAHHHGPVESVIYVVSGRARMRWGEKLEFMAEAGPGDFIY
                     VPPYVPHQEINASDDEPLSCVLVRSGQEPVVVNLDIPMVEKPEEVHWVDNIHPDPKKK
                     "
     gene            2602..>3442
                     /locus_tag="AC16CX_05195"
     CDS             2602..>3442
                     /locus_tag="AC16CX_05195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3011941.1"
                     /translation="MTTGTVRRLLASIGRITAAVLAGVVIGNPILLYFQQEKLLFYPD
                     SINEGNRRVIKERYRYVEEVTLTAADGTKLHGWFQHTPGRGRKPLLIYFGGNAEDLSF
                     LPTATDRVAGWSLLALNYRGYGLSEGRPGERVLFGDALHIFDAVARREDVDAGSIAIM
                     GRSLGSGVAAHLAAHRPVRAVILVTPYDSIASVAKEKFWFAPVSLILKHPFDSLALAP
                     KIDKPALFLVAGEDETIPLPHSRRLFDAWAGPKTWHLVPKENHDTIDADGDYWRVIHD
                     FLRG"
BASE COUNT          629 a         1064 c         1125 g          624 t
ORIGIN      
        1 gccgcggtga tacaccagcg cccctcgggc tactccttcc tcccggagct ggcggactgc
       61 gcgtgccagc aggtttcggt cgagaccgcg cagtccaatc tcgattgctc ggtgctggcg
      121 aagctcgcgg gcaagaaaat catggtcggc gccctggacc tgagcgacat gactgtcgag
      181 acgcccgcgg tggtcgtcgc ccgcatcaag cgcgcgctgc cctacgtcaa gcccgaagac
      241 gtgatcatcg cgccggactg cggcatgaag tacctgccgc gtgacgtcgc cttcggcaag
      301 atgcgcgcca tggtcgaagc ggcaaaggtc ctccgcgatg aatatggcgg aggaccctag
      361 tgaaggatga ggggtgaggg cggagggccg agggtgttca caacacccat cacgcgtcag
      421 ttaaccttga ttcccgcctg ccgcgcgatg tcggcccact tcttcttttc gtcgcggacc
      481 atcttgcgga actcgaccgg agtgatgatc cgcggctcgg ctgcctcgcc agcgagccgt
      541 ttctgagtct cgggctcccg gaggatggcc gtgatttcgc ggcgaatctt ctccagcacc
      601 gcgcgcggca cgcctgccgg cgcggcgacc ccccaccaga cgtagacttc gtagccggga
      661 aagccggcct cggcgacggt tggaatgtcg ggcaccgcca ggttgcgctt cgccgcgcct
      721 accgccagca tcttgatcct gcccgcgcgc acgtgcggca gcacctgggt gaccgaagtg
      781 aacatcaccg gcatgtttcc cgccatcacg tcgatcatcg cggggccgcc gcccttgtac
      841 ggcaccatca ccatgtcgat gccggcgacc tttttgaaga gctcaccgcc gaagtggttg
      901 aacccgccga cgccggtcga ggcgtattgg agtgcccccg gttttgcccg cgcgcgctcc
      961 acgagctcgc gcagcgagcc cacgcccagt cccgggaaca ccacgatcga attcggcgac
     1021 gatccgatca tcgagatcgg gtcgaacgat ttttccacgt cgtagggcaa cggccggagg
     1081 acggcgttca tcacccatga tgtcgaaacc atgagcaagg tatggccgtc ggcgggggca
     1141 ttggcggcga gcccggccgc gatgatccca ttcgcgccgc cgcggttgtc gatcacgacc
     1201 tgctggccca cgcggtcggt gagcttggcc ccgaggtagc ggccgagcat gtcgttgctg
     1261 cctcccggcg ggaacgggat gatccagcgg atcggcttgt tcgggaagtc gccggcttgc
     1321 ggctgcgcgg cggaatgcgc cgccggtaac gggagcgcaa gcaagctgag tacgaccgca
     1381 atcgaccggc tgcgcagatt catactgagg tcctcctcct cgatatcgcc gtggctgcac
     1441 agcaatccga ttttgaggaa aagtataggc tccggcacgg ttgagttcaa aataggcgat
     1501 gaggagggaa tgaacggggg gaccttatca aaagaggcgc tgtctcaaca ggttactggt
     1561 tgatctccac atcgcaggag ggcaccccct atgttgctgt ccctgcttat cgtcaccttt
     1621 gtcgtcgcct tgttcacgtc gtgggtcgtg gtgcggtttt tctccgcgcc ggtcgaggcg
     1681 atcctcaagc ggatcatcga cgatgaaatc cgctcggcgt ggctgaaata cctgaagttt
     1741 gcgatttacg tcgtcggcgt ttctaaaggc gtgcgggtgt tggagctgga acgctatgtc
     1801 acgcccagcc ggttcgacaa ggacgcgcgc attcttgaat tgaccgcgga gcgctggctg
     1861 ctcgaaattt accggacggt catcgagacg ctgcagggga tcgcatggat gctgctgatc
     1921 tttttcatct ttgcgctgat cgcgtatgtc atcgttcgcg tgttcgagct ccggcgcgga
     1981 tcgaagcctg aaacaggcga tggttgattg cgcgaggacc tggcacgcgg cagaccgaca
     2041 agcaggcgag aggctcgact acaataggct cgcgcggagg gcgacatggc gaacaacccg
     2101 tcggaatcca aatggaagtt ccacggcgtg cgcgtggtgc acgccaacga gcttgacatc
     2161 aacacgccgc agacgcccgg catgaaccgc gccgcggcgg tcacctacgc gcgcgccggc
     2221 gcggaaaagc tctgggccgg aaccgtggtg atccatccca aggccaaaac cggcgcgcac
     2281 caccacggtc cggtcgagag cgtgatctac gtcgtcagcg ggcgcgcgcg catgcgctgg
     2341 ggcgagaagc tcgagttcat ggcggaggcg gggccgggcg acttcatcta cgtgccgccg
     2401 tacgtgccgc accaggagat caatgcgagc gacgacgagc cgctctcctg cgtgctcgtg
     2461 cggagcggcc aggagccggt ggtggtcaat ctcgacatcc cgatggtgga gaagccggaa
     2521 gaagtgcatt gggtggacaa catccatccc gatcccaaga agaagtgatg agtaatgggt
     2581 gtgcagtaac gaagtgacgc agtgacgact ggcaccgtgc ggcgtctgct tgcaagcatc
     2641 ggcaggatca cagccgccgt gctcgccggc gtcgtgatcg ggaacccgat cctgctctat
     2701 ttccagcagg agaagctgct tttctatccg gactcgatca acgagggcaa tcgaagagtc
     2761 atcaaggagc gataccgtta cgtcgaggag gtcacgctca ccgcggcgga cgggacgaag
     2821 ctgcacgggt ggttccagca cacacccggg aggggcagga agccactgtt gatctatttc
     2881 ggtggcaacg ccgaggacct ctcgttcctt cccaccgcga cggatcgtgt cgcgggctgg
     2941 tccctgctcg cgctgaacta ccggggctac ggcctcagcg aaggcaggcc cggcgagcgg
     3001 gtcctttttg gagacgcgct gcatattttt gacgcggtcg cgcgccgcga ggacgtggac
     3061 gctggcagca tcgcgatcat gggcaggagc ctcggcagcg gcgtggcggc ccacctggca
     3121 gcgcaccggc cggttcgcgc ggtcattctg gtcacgccct acgacagcat tgccagcgtc
     3181 gccaaggaaa agttctggtt cgctcccgtg tccctgatcc tcaagcatcc gttcgattca
     3241 cttgcgctgg cgccgaaaat cgacaagccg gcgctgtttc tggtggcggg ggaggatgaa
     3301 acgataccgc tgcctcattc ccgccgccta tttgacgcgt gggccgggcc gaaaacctgg
     3361 catctcgtgc cgaaggagaa tcacgacacc atagacgccg acggcgacta ctggcgggtc
     3421 atccatgact ttctccgcgg tg
//
LOCUS       DBNGWZ010000174        10120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1528479, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000174 DBNGWZ010000000
VERSION     DBNGWZ010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10120
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1528479"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..460)
                     /locus_tag="AC16CX_05200"
     CDS             complement(17..460)
                     /locus_tag="AC16CX_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3011942.1"
                     /translation="MFRNILVPTDGSLLSRRAIKKAVQLAKEQNARVTGFYVGPSWNP
                     KVSEDIVSSGVVSPQQHAASVKKAATRILDAVKRAAAKASVPCTCSYSTGDYPYLRIV
                     EAAHANGCDLILMATHGRRGISRLLLGSETSKVLAHSKVPVLVCR"
     gene            complement(655..1611)
                     /locus_tag="AC16CX_05205"
     CDS             complement(655..1611)
                     /locus_tag="AC16CX_05205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR3011943.1"
                     /translation="MKTITDRIDNTTRIPPEYLKADPPAPRSVKIEISPRCNYRCGFC
                     ALRTREVQPKWDMDFGLFKRITREMREAGVEEIGVFYLGESFMNPRLLVDCITWLKRD
                     VGMPYVFLTSNASMSFPEAVEACMKAGLDSLKWSVNAADEVQFEKIMGVSGRLFRRAL
                     ENIKSAWKVRQLGGYRTGLYASSIRYDGEQQEKMEQLLAERVRPHVDQHYWLPLYSMG
                     AFATKREQELGYRPTAGNQGRIGALRDPLPCWSAFTEGHVTAEGKLSACCFDATAHWT
                     MGDLNRQSFMEAWNSEPFVRLRAAHLKRDVSGTVCRDCVAYQ"
     gene            1725..3167
                     /gene="ccoN"
                     /locus_tag="AC16CX_05210"
     CDS             1725..3167
                     /gene="ccoN"
                     /locus_tag="AC16CX_05210"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR3011944.1"
                     /translation="MPRGAAMQPQGTFNYKVVRQFAVMTVVWGVVGMLVGVIIAAQLI
                     WPELNLAPWLSYGRLRPLHTNAVIFAFGGSALFATSYYVVQRTCHVRLFSDALAAFTF
                     WGWQAVIVAAAVTLPLGITTSKEYAELEWPIDVLITLVWVAYAVVFFGTLAKRRVQHI
                     YVANWFFGAFILTIALLHVVNSAEIPVTLWKSYSVYSGVQDAMVQWWYGHNAVGFFLT
                     AGFLGMMYYFVPKQAGRPIYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGM
                     VFSLILLAPSWGGMINGIMTLSGAWEKLRTDPILKFLIVSLSFYGMSTFEGPLMSIKT
                     VNALSHYTDWTIGHVHSGALGWVGLVSMGSIYYLLPRLFGRTEMHSVRLINAHFWIAT
                     VGIVLYIAAMWIAGVMQGLMWRAVSADGTLTYTFVESVKASYPFYAIRLLGGLLYFAG
                     MLVMAYNMAKTIAGAKTVDAAIPVPATAHA"
     gene            3184..3795
                     /gene="ccoO"
                     /locus_tag="AC16CX_05215"
     CDS             3184..3795
                     /gene="ccoO"
                     /locus_tag="AC16CX_05215"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR3011945.1"
                     /translation="MFTHDMIERNQALMVVLVILVVAVGGLVEIVPLYFQKSTTEPVA
                     GLKPYTPLQLTGRDIYIREGCYNCHSQMVRPFRAEVERYGHYSVAGEFVYDYPFQWGS
                     KRTGPDLHRVGGRYSDEWHRLHLVNPRSVVPESNMPAYPWLQRSAANAEDIEAKMRAL
                     RVLGVPYTEDEIAKAKGELKGKTETDALIAYLQGLGLALRNVR"
     gene            3799..3999
                     /locus_tag="AC16CX_05220"
     CDS             3799..3999
                     /locus_tag="AC16CX_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017366.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase subunit 3"
                     /protein_id="HIR3011946.1"
                     /translation="MDMEWIRNVMTVAAFVTFLGILAWAWSGRNRARFEEASRLPFDD
                     GELDGDLRTAQLARALNHGGRP"
     gene            4001..4915
                     /gene="ccoP"
                     /locus_tag="AC16CX_05225"
     CDS             4001..4915
                     /gene="ccoP"
                     /locus_tag="AC16CX_05225"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HIR3011947.1"
                     /translation="MSDFSHDAWSVTIALISVISIVACAVFLKAQGVRKSVEADTTGH
                     TWDEDLTEYNNPLPRWWAWLFYITIAFSLGYLVLYPGLGGYPGVLGWTQLRQLEDETR
                     AADARFGPIYEKFATQEVAAVARSSEALAIGQKLFLNNCAQCHASDGGGSRGFPNLTD
                     KDWLWGGTPEAIKASITEGRTSVMPPMGAAVGGDEGAKDVAHYVLSLSGRTHDSLRAF
                     RGKTVFQQACVACHGPEGRGNQQLGAPNLTDGVWLHGSSEAAIIGQIVKGRTSQMPAH
                     KDILSPAKIHLLAAYVYSLSQGGAPPGQ"
     gene            4932..6425
                     /gene="ccoG"
                     /locus_tag="AC16CX_05230"
     CDS             4932..6425
                     /gene="ccoG"
                     /locus_tag="AC16CX_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HIR3011948.1"
                     /translation="MAQPSLEAVDITKNDKAAPPEEIQGGSGAVIETGLYEARKEIYP
                     RAVSGWFAAWRWALVWATQLVFYGGAWLTWNDRQAVLFDIVHRKFYIFALVFWPQDII
                     YLAVLLIISALSLFLFTAVAGRLWCGYACPQTVYTEIFLWIERVIEGDRLQRMKLNRG
                     DLSRRKLGLKAAKHAVWIAVALWTGFTFVGYFTPIHELWAEVMSLFAWIGGGPNPALG
                     PWEAFWMLFYGFATYGNAGWMREQVCIYMCPYARFQSAMFDKDTLIITYDRDRGEPRG
                     SRPRRAAAAALGLGDCVDCDICVQVCPTGIDIRDGLQYQCIGCAACVDGCDQVMDKMG
                     YPRGLIRYSTQNALEQKLTYARMLARAFRPRVLLYTVILWGVIIATAVTLWVRVPLKV
                     DVIRDRAAIAREVEGGQIENVYRLQIMNTAETRRTFDIRVEGPPTLHVASESLIELDP
                     TSSRMVPVRVRVQPWAAQPGTYRIAFVVSAVGAGGVTVREQSVFILR"
     gene            6450..6941
                     /locus_tag="AC16CX_05235"
     CDS             6450..6941
                     /locus_tag="AC16CX_05235"
                     /inference="COORDINATES: protein motif:HMM:NF017559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HIR3011949.1"
                     /translation="MNGEQKTSHEPRAASHRAKPWYREPWPWILMAGPAATIVAGAVT
                     IWLAMSSFDGLVADDYYKRGLAINQEVRRDQAAVDRGITANIETRGGMLRVRLEGRGP
                     APEALFALLVHATRAGHDQRLRLARVAPGVYESALPALPAGRWRLVLEDPRREWRIVK
                     EGL"
     gene            6938..7168
                     /locus_tag="AC16CX_05240"
     CDS             6938..7168
                     /locus_tag="AC16CX_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011950.1"
                     /translation="MKRAILILWPSFIVGGIGEVLFFTVFDPKELYLFGEPTSLSRLA
                     VYSIGFFLCWAFAAASSAFTCFLQRGADEVNR"
     gene            7570..7995
                     /locus_tag="AC16CX_05245"
     CDS             7570..7995
                     /locus_tag="AC16CX_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3011951.1"
                     /translation="MANITRFDPFTDMDDLFKGLFVRPMRLDLDLAPQMHVKMDVTKT
                     DDNYSVKAEMPGVKKDDIQVSIDGNQVTISGEIKKEKEEKKGEEVIRSERYYGKVSRS
                     FTLAHDVDEAKASAKYSDGVLQLTLPVKAKSSSKKLTVS"
     gene            8018..8266
                     /locus_tag="AC16CX_05250"
     CDS             8018..8266
                     /locus_tag="AC16CX_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011952.1"
                     /translation="MKARSPATRVRGRAGRTAAAGGLRPQVIVDEEQRRHLIECCAFF
                     RAQRFREVEPGRYRGEDLRAAAAAIDSVINPARKRKKR"
     gene            8263..8562
                     /locus_tag="AC16CX_05255"
     CDS             8263..8562
                     /locus_tag="AC16CX_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3011953.1"
                     /translation="MSVRRSLLSLAGAGFFVLPAATAGAELPNLERGRALYENHCVVC
                     HTPNVHRRVPRLPIDLRDLRLIVSSWTKQENLRWTEDDIEDVVHYLDRTHYRFEK"
     gene            8573..9415
                     /locus_tag="AC16CX_05260"
     CDS             8573..9415
                     /locus_tag="AC16CX_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR3011954.1"
                     /translation="MEVFGFDAFSPKEVAGRVNDFCVIKARLPLLTMWMLGMLAGAFI
                     GLGSIYYTLVVSDASLGFAAARVAGGVSFSLGLLLVVVAGAELFTGNNLLAMAWADGC
                     VSMREVLRNWVVVCAANFAGAAGLAMIVYLSGHWQMNGGAVGATYLKIAAAKAALPVW
                     GAFFLGVLCNILVCMAVWMALAGRSVVDKFVAILFPISAFVAAGFEHSIANMYFFPMA
                     LLLKWHGLALPPGGDAITVAGMLGNLGPVIAGNIVGGSVLVALVYWVIYLRGQPPRTE
                     APRS"
     gene            complement(9425..>10120)
                     /gene="fnr"
                     /locus_tag="AC16CX_05265"
     CDS             complement(9425..>10120)
                     /gene="fnr"
                     /locus_tag="AC16CX_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HIR3011955.1"
                     /translation="RVACSSCSLRELCLPAGLEAREMEKIDRLVNQRRRIRKGEHLYR
                     AGAPLAALYAVRSGFMKSCVLHEDGREQVAGFHMMGDLLGMDAIGGGRHMSDTVALED
                     TEVCEMPFPLLERLSQEVPSLQHQLHRVMSREIVRDYGVMLLLGSMKAEERLAAFLLN
                     LSQRFAARGYSPTEFNLRMTRQEIGSYLGLKLETVSRAFSSFQEQKLMEVRQRHVRIL
                     DLPALRALLGPTR"
BASE COUNT         1699 a         3218 c         3347 g         1856 t
ORIGIN      
        1 tcgcgtgcac gcgaaatcaa cggcagacca gcaccggcac cttgctgtgc gccagcactt
       61 tgctggtctc gctccccagc agcagccgtg atatgccgcg ccgcccgtga gtggccatca
      121 ggatcaggtc gcatccgttg gcgtgcgcag cttccacgat cctcaggtag gggtaatcac
      181 cggtgctgta ggagcaggtg cagggcacgc tcgcctttgc cgccgctctt ttcacggcgt
      241 cgaggatgcg cgtcgccgct tttttcacgc tggccgcgtg ctgctgcggc gatacaacgc
      301 cggacgaaac gatatcctcg ctcaccttcg gattccatga cgggccgacg tagaagccgg
      361 tgacgcgcgc gttctgctcc ttggcgagct gcaccgcctt cttgatcgcc cgacgcgaca
      421 gcagcgagcc gtcggtcggg accaggatat tcctgaacat gggttgtctc cttgtggcaa
      481 gcgcctacgc ggagcagtct agtcccgcgc ccgtcgcgca tcttgatcca gatcaagcgc
      541 tccgcccgtg agctgtaagc ggtgagccgt gagcggtgag cagtgtgccg agaaatcgag
      601 agaggcgagc cgggaacttc gcgaaaaacg cagatccaat ttaccgctta ccgctcattg
      661 ataggcaacg cagtcccggc acaccgtgcc gctcacgtcg cgcttgagat gcgccgcgcg
      721 gagcctgacg aacggctcgg aattccacgc ttccatgaag gactggcggt tgaggtcgcc
      781 catcgtccag tgggcggtgg cgtcgaagca gcacgccgag agcttgccct cggcggtgac
      841 gtggccttcc gtgaacgccg accagcacgg cagcggatcg cgcaacgccc cgatccggcc
      901 ctgattgccg gcggtggggc ggtagcctag ctcctgctcg cgcttggtgg cgaaggcgcc
      961 catcgagtag agcggcaacc agtagtgctg atccacgtgc ggccgcacgc gctcggcaag
     1021 cagctgctcc atcttctcct gctgctcgcc gtcgtagcgg atcgaggagg cgtaaagccc
     1081 ggtcctgtaa ccgccgagct gccgcacttt ccaggccgac ttgatgttct cgagcgcgcg
     1141 ccggaacagg cgccccgaga cgcccatgat cttctcgaac tggacctcgt cggccgcatt
     1201 gaccgaccac ttcagcgagt cgagcccggc cttcatgcac gcctcgaccg cctcgggaaa
     1261 ggacatcgag gcgttcgacg tgaggaatac gtacggcatg ccgacgtcgc gcttgagcca
     1321 agtgatgcag tccaccagca gccgcgggtt catgaacgac tcgccgaggt agaacacgcc
     1381 gatttcctcg acgcccgcct cgcgcatttc gcgggtgatg cgcttgaaca gcccgaaatc
     1441 catgtcccac ttcggctgca cctcccgcgt gcgcagcgcg cagaagccac aacggtagtt
     1501 gcagcgtggg gaaatctcga tcttgacgct cctgggagcc ggcgggtccg ccttcaggta
     1561 ttcgggcgga atccgagtcg tattgtcgat ccggtcggtg atcgtcttca tgcggcattt
     1621 cctgtcggct gccagcctgt aaccttaagc cattggcacg gccgcggctt gatatagatc
     1681 aaaatcggcc ttccgccccc tccttatcat cgcggcgtta cgccatgccc aggggggccg
     1741 cgatgcagcc gcagggaacg ttcaactaca aggtggtgcg acagttcgcg gtcatgaccg
     1801 tcgtctgggg cgtggtcgga atgctggtcg gcgtgatcat cgcggcccag ctcatctggc
     1861 ccgagctgaa cctcgcgccg tggctctcct acggccggtt gcggccgttg cacaccaacg
     1921 cggtgatctt cgccttcggc ggcagcgcgc tcttcgccac ctcgtactac gtcgtgcagc
     1981 gcacctgcca cgtgcggctg ttcagcgacg cgctcgccgc gttcacgttc tggggctggc
     2041 aggcggtgat cgtggccgca gcggtgacgc tcccgctcgg catcaccacg tccaaggaat
     2101 acgccgagct ggaatggccg atcgatgtcc tcatcacgct ggtatgggtc gcgtacgcgg
     2161 ttgtgttctt cggcacgctc gccaaacgcc gcgtccagca tatctacgtt gccaactggt
     2221 tcttcggcgc cttcatcctg acgatcgcgc tgctgcacgt cgtgaacagc gccgagatcc
     2281 cggtcacgtt gtggaagtcc tactccgttt attcgggcgt gcaggacgcg atggtgcagt
     2341 ggtggtacgg ccacaacgcc gtgggtttct tcctcacggc gggcttcctc ggcatgatgt
     2401 actacttcgt ccccaagcag gccggccgcc cgatctactc ctatcgcctg tcggttgtgc
     2461 acttctgggc gctgatcttc acctacatgt gggcgggccc gcaccacctg cattacaccg
     2521 cgctgcccga ctggacgcag tcgctcggga tggttttctc gctcatcctg ctggcgccgt
     2581 cctggggcgg gatgatcaac ggcatcatga cgctctccgg ggcgtgggag aagctgcgca
     2641 ccgacccgat cctcaagttc ctcatcgtgt cgctgtcgtt ctacggcatg tccacgttcg
     2701 agggtccatt gatgtcgatc aagaccgtga acgcgctctc gcactacacc gactggacga
     2761 tcggccacgt gcatagcggc gcgctcggct gggtcggcct ggtgagcatg ggctccattt
     2821 actacctgct gccgcgcttg ttcggtcgga ccgagatgca ctcggtgcgg ctgatcaacg
     2881 cgcacttctg gatcgcgacc gtggggatcg tgctctacat cgcggcgatg tggatcgccg
     2941 gggtcatgca gggcctgatg tggcgcgcgg tgagcgccga cggaacgctt acctacacct
     3001 tcgtggagag cgtgaaggcg agctatccgt tctacgccat ccggctgctc ggcgggctgc
     3061 tctacttcgc cgggatgctc gtcatggcct acaacatggc gaagacgatc gccggcgcga
     3121 aaacggtgga cgcggcgatc ccggtgcccg cgaccgcgca cgcctgagct ggggaggaga
     3181 gcgatgttta cccatgacat gatcgagcgc aaccaggcgc tcatggtcgt cctggtcatc
     3241 ctggtggtcg cggtcggcgg gctggtcgag atcgtgccgc tgtacttcca gaaatcgacg
     3301 accgagccgg tggcggggct caagccctac accccgttac agctcactgg acgcgatatc
     3361 tacatccgcg aaggctgcta caactgccat tcgcagatgg tgcgcccgtt ccgcgccgag
     3421 gtggagcgtt acggccatta ctcggtggcg ggcgagttcg tctatgacta tcccttccag
     3481 tggggctcca agcgaaccgg cccggacctg caccgggtgg gcgggcgcta ctccgacgaa
     3541 tggcaccggc tgcatctcgt caacccgcgc agcgtggtgc cggagtcgaa catgccggcg
     3601 tacccgtggc ttcaacggtc cgccgccaat gcggaggata tcgaggccaa gatgcgcgca
     3661 ctgcgtgtgc tgggcgtgcc gtacacggag gacgagatcg caaaagcgaa gggtgagttg
     3721 aagggcaaga ccgaaacgga cgcgctgatc gcctacctgc aagggctggg cctcgcgctc
     3781 cgcaacgtga ggtgagccat ggacatggaa tggatccgca acgtcatgac cgtcgccgca
     3841 ttcgtcactt tcctcggcat cctggcctgg gcatggagcg ggcggaaccg ggcgcgattc
     3901 gaagaagcgt cgcgattgcc gttcgacgac ggcgagcttg acggcgacct gcgcaccgcg
     3961 cagcttgcgc gcgcgctcaa ccacggcggc aggccctgag atgagcgatt tcagccacga
     4021 cgcgtggagc gtcaccatcg cgctgatcag cgtgatcagc atcgtcgcct gcgccgtttt
     4081 cctcaaggcc cagggcgtgc gcaaatcggt cgaggccgac accaccggac acacgtggga
     4141 cgaggacctc accgagtaca acaatccgct tccgcgctgg tgggcgtggc tgttctacat
     4201 cacgatcgct ttctcgctcg gttacctggt tctttatccc gggctcggcg gctatcccgg
     4261 cgtgctgggc tggacgcagc tgaggcagct ggaggacgaa acccgcgcgg ccgacgcccg
     4321 gttcggaccg atctacgaga agttcgccac gcaggaggta gccgccgtgg cgcggagctc
     4381 cgaggcgctg gcgatcgggc agaagctttt cctcaacaac tgcgcgcagt gccatgcctc
     4441 cgacggcggc ggcagccgcg gcttccccaa cctgaccgac aaggactggc tgtggggcgg
     4501 aacacccgag gcgatcaagg cctcgatcac cgaagggcgc accagtgtaa tgccgccgat
     4561 gggcgctgcg gtcggcggcg acgaaggcgc gaaggacgtc gcccattacg tgctctcgtt
     4621 gtccgggcgc acccacgact cattgcgcgc gttccgcggc aagactgtgt tccagcaggc
     4681 atgcgtggcg tgccacgggc cggagggcag gggcaatcag caactcggcg cgcccaatct
     4741 caccgatggg gtatggctgc acggctcgag cgaggcggcg atcatcgggc agatcgtgaa
     4801 gggccgcacg agccagatgc cggcgcacaa ggacatcctg tcgcccgcga agatccacct
     4861 gctcgcggcg tacgtctatt ccctgtccca gggcggtgcg ccgcctggcc agtagcgccc
     4921 ggggagaagg catggctcaa ccttccctcg aagcggttga cataacaaaa aacgacaagg
     4981 cggcgcctcc cgaagaaatc cagggcggga gcggggccgt catcgagacc gggctctacg
     5041 aggcccgcaa ggaaatctat ccgcgcgccg tgagcggctg gttcgcggcc tggcgctggg
     5101 cgctcgtctg ggccacgcag ctcgtgttct acgggggcgc gtggctcacc tggaacgacc
     5161 ggcaagcggt cctgttcgac atcgtccacc gcaagttcta tatcttcgcc ctggtgttct
     5221 ggccgcagga catcatttat ctcgccgtcc tgctcatcat ctcggcgctg tcgctgttcc
     5281 tgttcaccgc ggtggccgga cggctgtggt gcggctacgc ctgcccgcag acggtctaca
     5341 ccgagatctt tctgtggatc gagcgcgtga ttgagggcga ccgcctccag cgcatgaagc
     5401 tcaaccgcgg cgatctgtcg aggcgcaagc tcggcctgaa agcggcgaag catgcagtgt
     5461 ggatcgcggt tgcgctctgg accggcttca ccttcgtcgg ctacttcacc ccgatccacg
     5521 agctgtgggc ggaggtgatg agtcttttcg catggatcgg cggcggaccc aaccccgccc
     5581 tgggcccgtg ggaggcgttc tggatgctgt tctacggctt cgccacctat ggcaatgccg
     5641 gctggatgcg cgagcaggtg tgcatctaca tgtgcccgta cgcgcgcttc cagagcgcga
     5701 tgttcgacaa ggatacgctg atcatcacct acgaccggga ccggggcgaa cccaggggtt
     5761 cccggccgcg gcgcgcggcc gccgccgccc tgggcctcgg cgactgcgtg gactgcgaca
     5821 tctgcgtgca ggtgtgtccg acgggcatcg acatccgcga cggcctccag taccagtgca
     5881 tcggctgcgc cgcctgcgtg gatgggtgcg accaggtgat ggacaagatg ggctacccgc
     5941 gcgggctgat ccgctactcg acgcagaacg cgctcgagca gaagctcacc tacgcccgga
     6001 tgctcgcgcg cgcgttccgt ccgcgagtgc tcctctacac cgtgatcctg tggggcgtga
     6061 tcatcgcgac ggccgtcacg ctctgggtga gggtgccgct caaggtggac gtgatccgcg
     6121 accgtgcggc gatcgcgcgc gaggtcgaag gcggccagat cgagaacgtc taccgtctgc
     6181 agatcatgaa taccgccgag acccggcgca ccttcgatat tcgcgtcgaa ggcccgccta
     6241 cgctccacgt ggcgagtgaa tcgctgatcg agctggatcc gacgtcgagc cggatggtgc
     6301 cggtgcgggt acgcgttcag ccgtgggcgg cccaaccggg aacctaccgc atcgcgttcg
     6361 tcgtgagcgc tgtgggtgcc gggggcgtga cggtgcgcga gcaatccgtt ttcatcctga
     6421 ggtgaaccag catcagtgaa cggtaaatcg tgaacggtga acagaaaacg agtcacgagc
     6481 cacgagccgc gagtcaccgg gcgaagccct ggtatcgtga gccctggccc tggatcctga
     6541 tggccggtcc ggccgccacg atcgtcgctg gcgcggtgac gatttggctc gcgatgtcct
     6601 cgttcgacgg gctggtcgcc gacgactact acaagcgcgg actcgcgatc aatcaggagg
     6661 tccggcgcga ccaggcggcg gttgatcgcg gaatcaccgc gaatatcgag acgcgcgggg
     6721 gcatgctgcg ggtgcggctg gaaggccgcg gcccggcgcc cgaggcgctc tttgcgctgc
     6781 tcgtgcacgc aacgcgggca gggcacgatc aacggctgcg cctggcgcgc gtcgcgccgg
     6841 gcgtgtacga aagcgcgctg ccggcgctgc ccgcggggcg gtggcgtctg gtcctcgaag
     6901 acccgcggcg cgaatggcgg atcgtgaagg agggcctatg aagcgggcga ttctcatcct
     6961 gtggccgtcg ttcatcgtcg gcggcatcgg cgaggtgctg tttttcaccg ttttcgaccc
     7021 gaaggagctt tacctgttcg gtgagcccac gagcctgagc cggcttgcgg tgtactccat
     7081 cgggttcttt ttatgctggg cgttcgcggc ggcttcgagc gcgttcacct gcttcctgca
     7141 gcgcggcgcc gacgaggtaa accggtaatt caggaaccgc cgatgaacgc agataaacac
     7201 gaatccattt caaaaaacct gctgcgttgt tacttcctgc atcggcgttg atcagaggtg
     7261 gcagcagcaa tcatgttctt gattcttgag acaagttcca gccacattcg atggtaaaca
     7321 ggggcaggcg gcgagtaacc ccggataaac gctagcggag atcctcgccg atggtggttt
     7381 cgggaatcgt tgatctgcgt gcatctgcgt tcatctgcgg tttcactcgg tttttccatg
     7441 ccgcccgccc ttgatctagg tcaagaagcc cgaagcggcc gtcgctagta tggcgataga
     7501 cggcctgaat ggccgccagt ttgtgccgcg cagacgcggc tgattcaacg gattgtgcag
     7561 gagaaaatca tggcaaacat cacgcgtttc gatccgttca ccgacatgga tgatctgttc
     7621 aagggattgt tcgtgcgccc gatgcggctg gatctcgatc ttgccccgca gatgcatgtc
     7681 aagatggacg tcacgaagac cgacgacaac tatagcgtga aggcggaaat gccaggcgtg
     7741 aagaaggacg acatccaggt ctcgatcgac ggcaaccagg tgacgatctc gggcgagatc
     7801 aagaaggaga aggaggagaa aaaaggcgag gaagtaatcc gttccgagcg ctattacggc
     7861 aaggtgtccc gtagtttcac gctggcgcac gatgtcgacg aggcgaaagc cagcgccaag
     7921 tactcggacg gagttcttca gctgaccctg ccggtcaagg ccaaatcctc gagcaagaag
     7981 ctcaccgtca gctgaagccg gggcagtcga gaacgtcatg aaggcgcgca gtcctgcgac
     8041 acgcgtccgc ggcagggcgg gccggactgc tgcggcgggc gggctgcgtc ctcaagtgat
     8101 cgtggacgag gagcagcgcc ggcacctcat cgagtgctgt gctttcttcc gggcccagcg
     8161 cttccgggag gtcgagcccg gccgctaccg cggggaggat ctgcgggccg ccgccgcggc
     8221 catcgattcg gtgatcaatc cggcgaggaa gaggaagaaa cgatgagcgt gcgccggtcg
     8281 cttcttagcc tggccggtgc gggctttttt gtgcttcctg ctgccaccgc gggcgcggaa
     8341 ctgcccaacc tggagcgggg ccgtgccctc tacgagaatc actgcgtcgt atgccacacg
     8401 cccaacgtgc atagacgggt tccgcgtctg ccgatcgatc tccgggattt gcggctgatc
     8461 gtgtcttcgt ggacgaagca ggaaaatctg cggtggacgg aggacgacat cgaggacgtc
     8521 gtccattacc tcgaccgcac gcattaccgg ttcgaaaaat aggcttcccg ccgtggaagt
     8581 attcggattc gacgcgttct cgcccaagga ggtcgccggg cgcgtcaacg acttctgcgt
     8641 catcaaggcg cggctgccgc tgctgaccat gtggatgctc ggcatgctcg ccggggcctt
     8701 catcggcctg ggctcgatct attacaccct ggtggtgagc gacgcctcgc tcggcttcgc
     8761 cgccgcccgc gtggccggcg gcgtgagctt ctcgctgggc ctgctgctcg tcgtggtggc
     8821 gggcgcggag ctattcacgg gcaacaacct cctcgccatg gcctgggccg acggctgcgt
     8881 gagcatgcgc gaggtattgc gcaactgggt cgtggtgtgc gcggcaaatt tcgccggcgc
     8941 ggcgggactg gcgatgatcg tctacctctc gggccactgg cagatgaacg gcggcgcggt
     9001 gggcgcgacc tacctgaaga tcgccgcggc caaggccgcg ctcccggtgt ggggcgcgtt
     9061 cttcctcggc gtgctgtgca acatcctggt gtgcatggcg gtgtggatgg cgcttgccgg
     9121 gcgcagcgtc gtggacaagt tcgtcgccat cctgttcccg atctcggcct tcgtcgccgc
     9181 cggcttcgag cactcgattg ccaacatgta cttcttcccg atggcgctgc tgctgaagtg
     9241 gcacggcctg gcgcttccgc ccggcggtga tgccatcacc gtggccggca tgctgggcaa
     9301 tctcggcccg gtgatcgccg gcaacatcgt cggcgggagc gtcctggtcg cgctggtcta
     9361 ctgggtgatc tacctgcgcg gccagccgcc gcgcaccgaa gcgccgcgtt cctgaacgcc
     9421 acggtcagcg cgtggggccg agcaaggccc tgagggcggg gagatcgagg atgcgcacat
     9481 gtctttgccg cacctccatc agcttctgct cctggaagct ggagaaggcg cggctcacgg
     9541 tctcgagctt gagcccgagg tagctgccga tttcctggcg cgtcatgcgc aggttgaatt
     9601 cggtcgggga atagccgcgc gcggcgaagc gctgggacag gttcagcagg aaagcggcca
     9661 gccgctcctc cgctttcatg ctgccgagca gcagcatgac gccgtagtcg cggacgatct
     9721 cccggctcat gacgcgatgc aactggtgct gcagcgaggg gacttcctgg gagagccgct
     9781 cgagcagcgg gaagggcatc tcgcacacct cggtatcctc cagcgccacc gtgtcgctca
     9841 tgtggcggcc gccaccgatc gcgtccatcc cgagcaggtc gcccatcatg tgaaagccgg
     9901 cgacctgctc gcgcccgtcc tcgtgcagca cgcagctctt catgaagccc gaccggacgg
     9961 catagagcgc ggccagcggc gcgccggcgc ggtagaggtg ctcgccttta cggatccggc
    10021 gccgctgatt gacgagccgg tcgattttct ccatttcccg cgcctcgagc cccgccggca
    10081 ggcacagttc ccgcaggctg cacgaggagc aggcgacgcg
//
LOCUS       DBNGWZ010000175         3950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2410960, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000175 DBNGWZ010000000
VERSION     DBNGWZ010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2410960"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="AC16CX_05270"
     CDS             <1..580
                     /locus_tag="AC16CX_05270"
                     /inference="COORDINATES: protein motif:HMM:NF022673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HIR3011956.1"
                     /translation="VRERSWHDLFNVLAWLAYPGAKGALNARHYAALEAQRAPNRGPT
                     QDALTLFDEGGVIVASSDDELLGLLRRWCWKELFWGNRSKLAARMRFFLFGHALYEKA
                     LRPFTGITGRGILLRAEPKLIAAPLAEQIGVIDARISEHIADAGHLIGTRELAVVPVL
                     GVPGWCADNERESYYDNLDYFRPGRLKEMRDK"
     gene            complement(672..1727)
                     /locus_tag="AC16CX_05275"
     CDS             complement(672..1727)
                     /locus_tag="AC16CX_05275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3011957.1"
                     /translation="MQTQLIFDPALSAEELRGFARTVAEIEWLLLILVMLYQVVLAPN
                     GEVTAALSMAMFFFAAFVLSFHYANFYRNETPWKIALETWVMIVFITWVLAYTGGPES
                     PLRDLYILVIIVSALTLGKLATLLEMALIAACYVWLGYPQQREELLSLPFGARLLAQI
                     SPMLLVAYITTMLSADIRRAYGHMKVLSETDELTGVLNMRAFSTTAERVFHQAERYAR
                     PFSVLMIDSDSLKTVNDNHGHETGNRLLRTTVECIQGQLRQTDLIARYGGDEFVVLLP
                     ETPCSGAVGVAERIRKSMESTLLPTREQAVRATVSIGISCYPNHGPDFETILDKADQA
                     MYASKAGGKNRTTMFSA"
     gene            complement(1782..2897)
                     /locus_tag="AC16CX_05280"
     CDS             complement(1782..2897)
                     /locus_tag="AC16CX_05280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3011958.1"
                     /translation="MGTETPSPPQPAGASARKPGTDARPPGDLDIESTELRDFSRTVA
                     EIEWLLVILVLLYHVFQDRSEDNTVAIYSGLIVFSAVIIAFHYFNFVRKPGRWSLAVE
                     TWIMIVFITWVLYHTGRLDSPLLNLYLLPIVTSALTLGQKATLLQVGLIGACYLLLGY
                     TTNVSFISIITAGDFAAGLAPMLLVAYITTMLSQDILNAMAKIKLLSETDELTRVYNM
                     RAFTAIAQRECALAGRYNRTFSLLMVDSDNLKKVNDSHGHEVGDQLIQHIVQCIRDSL
                     RVTDVVARYGGDEFMCLLQEAGNEAAAQVAERIRSRIAGTALNAGRDAVQTSVSIGVA
                     TYPEHGDKFDALAKNADRALYASKAQGRNRVTVYSSD"
     gene            complement(3084..>3950)
                     /locus_tag="AC16CX_05285"
     CDS             complement(3084..>3950)
                     /locus_tag="AC16CX_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3011959.1"
                     /translation="MLLAFDRHHAADGTPLPEQTAFRVPDAYAMATARRYPRYFEWVA
                     SIHPYRRDGVEALRKAAADGARAVKWLPAAMGIDPSSPRCDAYYGELKKLDLPLITHA
                     GMERAVSVGDRQHLGNPLLLRRPLDHGVRVVAAHCASMGEDRDLDRGANGPYVESFAL
                     FARLMDDPRYGRTLYGDVSAVAQTNRAGPALVRLIEREDWHARLLNGSDYPLPGIMPL
                     YSMSQLTQLGLIEADLAPVLAAIREHNPLLFDFVLKRHLRAGNKRFAAKIFETRPFFD
                     RKLSAVSVQPPA"
BASE COUNT          685 a         1209 c         1351 g          705 t
ORIGIN      
        1 ggtgcgcgag agaagctggc acgacctctt caacgtgttg gcgtggcttg cctaccctgg
       61 cgccaagggc gcactcaacg cgcgccacta cgcggcgctc gaagcgcagc gtgcgcctaa
      121 ccgtggcccc acgcaggacg cgctcacgct gttcgacgaa ggcggggtga tcgtggcctc
      181 gagcgacgac gagctgctcg ggctgttgcg gcgatggtgc tggaaggagc tgttctgggg
      241 caaccgctcg aagctggcgg cgcggatgcg ctttttcctg ttcggacacg cgctctacga
      301 gaaggccctg cggcctttca ccggcatcac cggccgtggc atcctgctga gagcggagcc
      361 gaagctgatc gcggcgccgt tggctgagca gatcggcgtc atcgacgcga gaatctcgga
      421 gcatatcgcc gacgccggac acctcatcgg gacccgcgag ctggccgtgg tgccggtgct
      481 gggcgtgccg ggctggtgcg ccgacaacga gcgggaatcg tattacgaca acctggacta
      541 cttccgtcca ggtcgtctca aggaaatgcg tgataagtga taagtgataa gtgataagaa
      601 cggccggcat cgccggcctg ctagttcctg tcacctatca cctatcacct gtcactccat
      661 gttttccgcc gtcaggcgga aaacatcgtg gtgcggttct tcccccctgc cttgctcgcg
      721 tacatcgcct ggtcggcttt gtccaggatg gtctcgaaat ccgggccgtg gttgggatag
      781 caggagatgc cgatgctgac cgtagccctg accgcctgct cgcgcgtcgg gagcagcgtc
      841 gactccatgc tcttgcggat gcgctccgcg acgccgaccg cgccgctgca cggcgtctcc
      901 ggcagcagca ccacgaactc gtcgccccca tagcgcgcga tgaggtcggt ctggcgcagc
      961 tggccctgga tgcattccac cgtggtccgg agcagccggt tgccggtctc gtgtccgtgg
     1021 ttgtcgttca ccgttttcag gctgtcggag tcgatcatga gcacgctgaa cgggcgcgcg
     1081 tagcgctcgg cctggtgaaa cacgcgctcc gccgtcgtgc tgaaggcccg catgttgagc
     1141 accccggtga gttcgtcggt ttcggacagc accttcatgt ggccataggc gcggcgaatg
     1201 tcggccgaca gcatggtggt gatgtaggcg acgagcagca tcggcgagat ttgggcgagc
     1261 agccgggcgc cgaacggcag cgacagcagc tcttcgcgct gctgcggata gccgagccac
     1321 acgtagcatg ccgcgatcag cgccatttcg agcagcgtgg cgagcttgcc cagggtcagc
     1381 gcgctgacga tgatcaccag gatgtagagg tcgcgcagcg gactttcggg tccgccggta
     1441 tacgccagca cccaggtgat gaatacgatc atcacccagg tctcgagcgc gatcttccag
     1501 ggggtttcat tgcggtagaa attggcgtag tggaaggaca gcacgaaggc ggcgaagaaa
     1561 aacatcgcca tcgacaaggc ggccgtcacc tccccgtttg gcgccagcac cacctgatag
     1621 agcatcacca ggatcagcag cagccattcg atctccgcca cggtgcgcgc gaacccgcgc
     1681 agctcttcgg cggagagcgc gggatcgaag atcagctggg tctgcatgcc gatggaatag
     1741 gagctcgtgg cgtctcaggc cgcaagcata acaccggcgc gtcaatcgga tgagtacacc
     1801 gtcacgcggt tcctgccctg ggccttgctg gcgtagagcg cgcggtcggc gttcttggca
     1861 agcgcatcga atttatcccc gtgctcgggg taggtggcga cgccgatgct gaccgaggtc
     1921 tggactgcat cccgcccggc gttgagcgcc gtgccggcga tgcggctccg gatgcgctcg
     1981 gcgacctggg cggccgcctc gttccccgcc tcctgaagca ggcacatgaa ctcgtccccc
     2041 ccgtagcggg ccacgacgtc ggtcacgcgc agactatccc ggatgcattg aacgatatgc
     2101 tggatgagct ggtctccaac ctcgtgcccg tgactgtcgt tcaccttctt caagttgtcg
     2161 gaatcgacca tcagcaggct gaaggtgcgg ttgtagcgcc cggcgagcgc gcactcgcgc
     2221 tgggcgatcg cggtgaacgc gcgcatgttg tagacgcgcg tcagctcgtc ggtttccgag
     2281 agcagcttga tcttcgccat cgcgttgagg atgtcctgcg acagcatggt ggtgatgtag
     2341 gcgaccagca gcatcggcgc gagacccgcc gcgaaatcgc ccgcggtgat gatcgagatg
     2401 aacgagacgt tggtcgtgta gccgaggagc aggtagcagg cgccgatcag gcctacctgc
     2461 agcagcgtcg ccttctggcc cagggtgagg gcgctggtga cgatcggcag caggtagaga
     2521 ttgagcagcg ggctgtcgag gcgcccggtg tggtagagca cccacgtgat gaacacgatc
     2581 atgatccagg tctcgaccgc gagcgaccag cgccccggct tgcggacgaa gttgaagtaa
     2641 tggaatgcga tgatgaccgc cgagaagacg atcagcccgc tgtagatggc gacggtgttg
     2701 tcctcggagc ggtcctggaa tacgtgatag agcaggacca ggatcaccag cagccattcg
     2761 atctcggcca ccgtgcgcga gaagtcccgc agctcggtgg actcgatgtc gagatcgccg
     2821 ggcgggcggg cgtccgtgcc gggtttgcgg gccgacgcgc cggccggttg cggcggtgag
     2881 ggagtttcgg ttcccaatgg cggggacgcc tcggcgaatt atcctgcttg aggggaaaac
     2941 ccttgttgtt gactggctgg cccgataggg cgcatcgagg tcgggctatg ttaatcgaaa
     3001 tgctcccgga tggggaggct ggggcccagc catcagccgt cggctttcag ccgtcggctc
     3061 aacccggaaa caccgcgaaa gcttcaagcc ggtggctgaa cgctgaccgc tgagagcttt
     3121 ctgtcgaaga acggccgggt ctcgaagatt ttcgccgcaa agcgcttgtt gccggcgcgc
     3181 aggtggcgtt tgagcacgaa atcgaacagc agagggttgt gctcgcggat tgcggccagc
     3241 acgggagcaa gatcggcttc gatcagaccg agttgcgtga gctggctcat cgagtaaagc
     3301 ggcatgatgc cgggcagcgg gtagtcggag ccgttgagca ggcgcgcgtg ccaatcctcg
     3361 cgctcgatca gcctcaccag cgccggcccg gcgcggttcg tctgtgccac cgcggagacg
     3421 tcgccgtaga gcgttcttcc atagcgcgga tcgtccatca gccgcgcgaa cagcgcgaag
     3481 ctctcgacgt aggggccgtt tgctccgcgg tcgaggtcgc ggtcctcgcc catgctggcg
     3541 cagtgcgcgg ccacgacgcg cacgccgtga tcgagcgggc gccgcagcaa cagggggttg
     3601 ccgagatgct ggcggtcgcc gacgctcacc gcgcgctcca tgcccgcgtg agtgatgagc
     3661 ggcagatcga gtttcttcag ctcgccgtaa taggcatcac aacggggcga ggaggggtct
     3721 attcccattg ccgcgggcag ccacttgacc gcgcgggcgc cgtcggccgc cgccttgcgt
     3781 agcgcttcca cgccatcgcg ccggtaggga tggatggagg cgacccactc gaaataccgc
     3841 ggatatctcc gcgccgtcgc cattgcgtat gcgtcgggga cgcggaaagc ggtctgttcc
     3901 ggcaatggcg ttccgtccgc ggcatgatgc cggtcgaacg cgagcagcat
//
LOCUS       DBNGWZ010000176         4782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1276844, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000176 DBNGWZ010000000
VERSION     DBNGWZ010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1276844"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1295)
                     /locus_tag="AC16CX_05290"
     CDS             complement(141..1295)
                     /locus_tag="AC16CX_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3011960.1"
                     /translation="MGEVVVSAVIPCLDEERTIGACIEKARASFAVLGLTGEVVVVDN
                     GSTDRSAEIAREMGARVVPHPVKGYGAALGRGIEESRGRIIVMGDADDSYDWSAIGPF
                     IRKVEEGYDLVVGNRFQGGILPGAMPVLHRYFGNPLLSAITRLAFGVSIGDVHCGMRA
                     FTKAAFERMGTGARGMEFATEMVANAAHQGLRITEIPTRLYPDRRERPPHLRTFRDGW
                     RHLRFILTYAPDYLYLAPGAVLLMAGLALQALLVAGPVDLGGVHLGIHYLALGAMLSL
                     VGFNVINLGVLAKALMAQRYSGLKSRTLSLISRRFTLEGGLVAGALLMIVGLTIDGVI
                     LAIRLAHPGEAMEETVHLAFVATTTVVLGLNLVFSSFLLAMVVTSRNEDS"
     gene            complement(1295..2866)
                     /locus_tag="AC16CX_05295"
     CDS             complement(1295..2866)
                     /locus_tag="AC16CX_05295"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3011961.1"
                     /translation="MRSRVLIIGAGPAGLTAAYELASAGVSDVVVLEATREVGGLARS
                     VNYKGNRIDIGGHRFFSKSDWVMNWWLRMLPLAIHPSPAPELELAYQGMRRMLPEAPT
                     ASESDPRVMLVRRRLSRIYFDGKFFDYPLKADLETALKLGLWRCTTFAASYVWAKLFP
                     RRPERSLEDFFINRFGSRLYRQFFKEYTEKVWGTPCDRISAQWGAQRVKSLSIGKALR
                     HALLKPFRRRADGFGAAQQTSLIERFLYPKYGPGLMWEVTAERLRERGVKIIFDATVK
                     HIDHSGGRIRAVRVAGPDGVEQCYEADCFVSSMPVRELVLGLSPAAPQRPREVAAGLE
                     YRDFVIVGLLLRRLMRTPAGRGSLNLVPDNWIYVQEPGVKVGRLQVFNNWSPYLVRDP
                     DTVWIGMEYFCREGDELWQTPDRELLRFGVAEMQQLRLADPDDYLDGVVVRMPKAYPA
                     YYGTYAHFGELREYLDAFPNLFLIGRNGMHRYNNQDHSMLSAKMAAGAILAGSADKSR
                     IWGVNIDDEYQEEVS"
     gene            complement(2879..3283)
                     /locus_tag="AC16CX_05300"
     CDS             complement(2879..3283)
                     /locus_tag="AC16CX_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR3011962.1"
                     /translation="MTGRIFRNVAGEGARYFAASAIALAADFGAYVALIRLAGVYYLV
                     AAPIGFALGLAVIYLLSIGWVFGERRLADVRMEFAIFASIGITGIGLNQLVIYVGVEL
                     LSLSFEFAKLASAAVVFCFNFAARKALLFTRY"
     gene            complement(3283..>4782)
                     /locus_tag="AC16CX_05305"
     CDS             complement(3283..>4782)
                     /locus_tag="AC16CX_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011963.1"
                     /translation="DDSVSYLVMAQVFSPWQPASPPVAEAFAREAFYPPLFPVLLAVT
                     GAAHDFALAHAVTALVLAACLPVLYVLGVRWLGSKWAAAAAAAVTALLPSLWIHVKGI
                     LSEPLFCLLLIATLCVLEIERDGRRKTLALALLMAALVLTRTVGLVVVTAYAMWALVQ
                     RDAPLATRARALLPALAAAATYAAWALLRPAGTTDNYTAIVVERAQDYFRAESPLAVF
                     GSSLLRQANSLAEAWSGAVLLFWVEGRPLRVLLAGAVGVLALAGMTLRLVAAKPDAWM
                     IAAYLATFLLWPFYDQMTRFLFPVLPVLVLYAFWAGRVALRALGRPPVLAYGVLVLLF
                     ASLAAPALGFIHERAKAGGRYGEMTDWYRTPDLDEARSRASVHLDLMADMEAIRRLTR
                     PGDRVMWVAPSYIALLADRRGVPAPLSELAPADYRRAVREAKPDFVFLSAYHPRDTIR
                     DAAWRAGMAALREDAQIVHVRRRADGSVSSLLLRAGELALVVRHERAGL"
BASE COUNT          891 a         1663 c         1546 g          682 t
ORIGIN      
        1 ttcctgcgcg gcacggtcat cttcggctcg cgcggcgccc aggcggacgc cgcgctgatg
       61 gccgcacgct ttccgcaata cgagcggctg tccgaggacc ggcgcggatc ggtgtggggc
      121 atcgcgcgcc gctgaacggc tcaggaatcc tcgttccgcg acgtcacgac catcgcgagc
      181 aggaacgagc tgaataccag attgagcccc aacacgacgg tggtggtggc gacgaacgcg
      241 agatgcacgg tctcctccat cgcctcgccc ggatgcgcca gccggatcgc gagtatcacg
      301 ccgtcgatcg tcagcccgac gatcatgagc agcgccccgg cgaccaatcc cccctcgagg
      361 gtgaagcgcc ggctgatcag gctgagggtg cggctcttga gtccggaata gcgctgcgcc
      421 atcagcgcct tcgcaagcac gcccaggttg atcacgttga acccgacgag cgacagcatg
      481 gcgccgagcg ccagatagtg gatgccgagg tgaacgccgc ccaggtcgac cgggccggcc
      541 acgagcaacg cctgcagggc gagccccgcc atgagcagca ccgcgcccgg agcgaggtaa
      601 aggtagtccg gcgcgtaggt caggatgaac ctcaggtgcc gccagccgtc gcggaacgtc
      661 cgcaggtggg gcgggcgctc gcgcctgtcg ggataaaggc gggtcggaat ttcggtgatc
      721 cgcaacccct ggtgcgccgc attcgcaacc atttcggtcg cgaactccat gccgcgcgcg
      781 ccggtcccca tgcgctcgaa cgcggctttc gtgaaggcgc gcatgccgca gtgcacgtcc
      841 ccgatgctca cgccgaaggc aaggcgcgtg atcgccgaca gcagcgggtt tccgaaatag
      901 cggtgcagga cgggcatcgc ccccggaagt atgccgccct ggaagcgatt gccgacgacc
      961 agatcgtagc cctcctcgac cttgcggatg aaaggaccga tcgccgacca gtcgtaggaa
     1021 tcatcggcgt ctcccatcac gatgatgcgg ccgcggctct cctcgatccc cctcccgagc
     1081 gcggcgccgt agcccttgac cggatggggg acgacgcggg ctcccatttc ccgggcgatc
     1141 tcggcggaac ggtcggtcga gccgttgtcc acgacgacca cctcccccgt cagccccagc
     1201 acagcgaagc tggccctggc cttctcgatg caggcgccga tggtccgctc ctcgtccagg
     1261 cagggaatga cggccgaaac cacgacctcg cccactatga aacctcctcc tggtactcgt
     1321 cgtcgatatt gacgccccag atgcgggatt tgtccgcgct gccggcaaga atggcgccgg
     1381 cggccatctt ggcggagagc atcgagtggt cctggttgtt gtagcggtgc atgccgttgc
     1441 gcccgatcag gaagagattc ggaaacgcgt caagatactc gcgcagttcg ccgaaatgcg
     1501 cgtaagtgcc gtaataggcg ggataggcct tcggcatccg gaccaccacg ccgtcgagat
     1561 agtcgtcggg atccgcgagc cgcagctgct gcatctcggc gacaccgaac cgcagcagct
     1621 cgcggtccgg cgtttgccac agctcgtcgc cctcgcggca gaagtactcc atgccgatcc
     1681 acacggtatc cgggtcgcgc accaggtagg ggctccagtt gttgaacacc tgcagccgcc
     1741 cgaccttgac gcccggttcc tggacataga tccagttgtc cggcaccagg ttgagcgaac
     1801 ccctccccgc cggcgtgcgc atgagccgcc gcaacagcag cccgacgatg acgaagtccc
     1861 ggtattcgag cccggcggcc acctcccggg gacgctgcgg cgctgcgggc gagagaccca
     1921 gcacgagctc ccgcacgggc atgctggaca cgaagcaatc cgcctcgtag cattgctcca
     1981 cgccgtccgg cccggccacg cgcaccgcgc ggatccgtcc cccgctgtgg tcgatatgtt
     2041 tcaccgtcgc atcgaatatg atcttcacgc cgcgctcgcg caaccgctcg gcggtgacct
     2101 cccacatgag ccccggcccg tatttcggat aaaggaagcg ctcgatcaac gaggtctgtt
     2161 gcgccgcccc gaagccatcg gcgcggcggc ggaacggttt gagcagcgcg tggcgcagcg
     2221 ccttgccgat ggacaggctc ttcacgcgct gtgcgcccca ctgcgcgctg atccggtcgc
     2281 acggcgttcc ccacaccttc tcggtgtatt ccttgaagaa ctggcggtag aggcggctgc
     2341 cgaagcggtt gataaaaaaa tcctcgaggc tgcgctccgg gcggcgcggg aacagcttcg
     2401 cccagacata gctcgccgcg aacgtcgtgc agcgccacaa ccccagcttg agcgccgttt
     2461 cgaggtcggc cttcagcggg tagtcgaaga attttccgtc gaaatagatg cgcgacaggc
     2521 gcctgcgaac cagcatcacg cgcgggtcgg attcgctcgc tgtgggcgct tcaggcagca
     2581 tgcgccgcat gccctggtag gccaactcca gctccggggc gggcgatgga tggatggcca
     2641 gcggcagcat gcgcagccac cagttcatga cccagtccga cttcgagaag aaccggtggc
     2701 cgccgatgtc gatgcggtta cccttgtaat tgaccgagcg cgccaggccg cccacctcgc
     2761 gcgtggcctc gagcacgacg acatcgctca ccccggcgct cgccagttcg taggccgccg
     2821 tcaggccggc aggtccggca ccgataatga gcacccggct gcgcatcgtt ccgcccgcct
     2881 agtacctcgt gaacagcagg gccttccggg ccgcgaagtt gaagcagaac actacggccg
     2941 ccgacgccag cttggcgaat tcaaaggaaa gcgagagcag ctctacgccg acgtagatga
     3001 cgagctggtt gagcccgatt ccggtgatgc cgatggaggc gaaaatcgcg aattccatgc
     3061 gaacgtccgc gaggcggcgc tcgccgaata cccagccgat gctgagcagg tagatcacgg
     3121 ctaaccccag cgcgaatccg atcggggcgg cgaccaggta atacacgccc gccaggcgaa
     3181 tgagggcgac atacgcgcca aagtccgccg cgagggcaat tgcggaagcg gcgaagtaac
     3241 gcgcgccctc gcccgccaca ttgcgaaata tcctgcccgt catcagagcc ccgcccgctc
     3301 atggcgcacg acgagcgcca gttccccagc cctcagcagg agcgagctga ccgatccgtc
     3361 ggcgcggcgg cgcacgtgca cgatctgcgc atcctcacgc agcgcggcca tccccgcgcg
     3421 ccacgccgca tcgcgtatcg tgtcccgcgg gtggtacgcg ctcaggaaaa cgaagtccgg
     3481 tttcgcctcg cgcacggccc gccggtagtc cgcgggggca agctccgaca acggcgcggg
     3541 aacgccgcgc cggtcggcga gcagcgcgat gtaggacggc gcgacccaca tgacgcggtc
     3601 ccccggccgg gtgagcctgc gaatcgcttc catgtcggcc atgagatcga gatgcacgct
     3661 ggcccgcgat cgcgcttcat ccagatcagg cgtgcggtac cagtccgtca tttcgccgta
     3721 gcggcctccg gccttggcgc gctcgtggat gaacccgagc gccggtgccg ccaggctggc
     3781 gaacagcagc acaagcaccc cgtacgcaag gacagggggc cgtccgagtg cgcgcagcgc
     3841 tacccggccc gcccagaatg catacaggac cagcaccggc agcaccggga agagaaaacg
     3901 cgtcatctga tcgtagaacg gccacaacag aaacgtcgcc agatacgccg cgatcatcca
     3961 agcatcgggc tttgccgcga ccagccgcaa agtcattccg gcaagcgcga ggacgccgac
     4021 ggcgccggct aggaggacgc gcagcgggcg gccttcgacc cagaacagca gcacagcgcc
     4081 ggaccaggcc tcggccagcg aattcgcctg cctgaggaga ctgctgccga aaaccgccag
     4141 cgggctttcc gcgcgaaaat aatcctgtgc gcgctcgacg acgattgcgg tgtagttgtc
     4201 ggtcgttccg gccggacgga gcagcgccca ggccgcgtac gtggctgccg cggcaagcgc
     4261 cggcagcagc gcgcgggcac gcgtcgcgag cggcgcgtca cgctgcacca gcgcccacat
     4321 cgcgtatgcg gtgacgacga ccagccccac cgtccgcgtc aataccaacg cggccatcag
     4381 aagcgccaga gccagtgtct tgcgccggcc atctcgctcg atttccagga cgcacagcgt
     4441 ggcgatcagc agcaggcaga acagaggctc gctcaaaatg cccttgacat ggatccacag
     4501 cgacggcagc agcgcggtga ccgcggccgc ggccgcggcc gcccacttgc tgccgagcca
     4561 gcgtacgcca aggacataga gcacgggcag gcaggccgcg agaacgagcg ccgtgaccgc
     4621 gtgcgccagg gcgaaatcgt gcgcggcccc ggtcaccgcc agcaacaccg gaaatagcgg
     4681 cgggtaaaat gcctcgcgcg cgaacgcctc ggccacaggt ggcgatgccg gctgccacgg
     4741 cgaaaaaacc tgcgccatca cgagatagct aacgctgtcg tc
//
LOCUS       DBNGWZ010000177         2271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_647108, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000177 DBNGWZ010000000
VERSION     DBNGWZ010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_647108"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="AC16CX_05310"
     CDS             complement(<1..1231)
                     /locus_tag="AC16CX_05310"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3011964.1"
                     /translation="MAEIKFGLRLRVALALSLACLLVVGALGFTLYTASEEMEESLID
                     QLVLEEMDYLVRRHRQDPGYQPQQSSNLQSYILSGASSEAQLPAFLTGLAPGRHEFFI
                     GGDEYHVLVREAGATRYIVAYEVGLHEQREQEFKLLTLLSVLTGAAASLVFGYWLSGV
                     LVLQVTELADQVGMLKPGQQQAALVRPGQDPEVSMLARAFEDYQARIEQMIRREQEFT
                     SNASHELRTPLTAIRTSCELLLADPALTGKSRARVEWINAAASRMAEQIQALLFLARG
                     QALGEVEPVALVDCVAEAVEPYRGEIARKGLMLEMDVARDAVLALDYHALRLVLSNLV
                     RNAVHYTERGFVKIGYAAKRLTVADSGQGIDADHLPRVFERFFRAEDMGGGTGLGLAI
                     VKRVCDHYGWKIEVASTP"
     gene            complement(1218..1901)
                     /locus_tag="AC16CX_05315"
     CDS             complement(1218..1901)
                     /locus_tag="AC16CX_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3011965.1"
                     /translation="MRVLIIEDDPAIAANLYDFLEAGGHVVDAAADGVTGLHLAVTGK
                     FDAIILDLGLPGMDGTTVCRKLREEAHDDTPVLMLTARDTLEDKLGGFALGADDYLVK
                     PFALKEVEARLAALHRRRAGRVTARPLTAGDLVFDPAGLTARFRGKEVRLPPKCIRIL
                     QVLMAQPNRVFSRKELEAEVWGEQQETSDTLRSHMHVLRRELVQAGGYDPIENVHGLG
                     YRLVPRGGD"
     gene            complement(2000..>2271)
                     /locus_tag="AC16CX_05320"
     CDS             complement(2000..>2271)
                     /locus_tag="AC16CX_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR3011966.1"
                     /translation="ALRVVSIVDELQDGLSSVYTFFDPDVRGASYGTYNILWQIGLCR
                     RLDLPYLYLGYWIAQSRKMAYKIHFQPMQGLVSGRWQPVIPPRKA"
BASE COUNT          396 a          742 c          760 g          373 t
ORIGIN      
        1 acggggtgct cgcgacctcg atcttccagc cgtagtggtc gcacacgcgc ttgacgatcg
       61 cgagcccgag tccggtgccg ccgcccatgt cttccgcgcg gaaaaaccgc tcgaagacac
      121 gaggcaggtg atcggcatca atgccctgcc cggaatcggc gaccgtcagg cgcttcgccg
      181 cgtagccgat tttcacgaag ccgcgctcgg tgtagtggac ggcgttgcgc accaggttgg
      241 agagcaccag gcgcagcgcg tgatagtcga gtgcgagcac ggcatcgcgc gcaacgtcca
      301 tctcgagcat caagcccttg cgtgcgatct cgccgcggta cggctcgacc gcctcggcca
      361 cgcagtccac gagcgcgacc ggctcgacct cgccaagcgc ctgtccgcgc gcgaggaaca
      421 gcagcgcctg gatttgttcc gccatgcggg aggctgcggc attgatccat tccacgcggg
      481 cacgcgactt cccggtcagc gccggatcgg cgaggaggag ctcgcagctg gtcctgatcg
      541 cggtgagggg ggtcctcagc tcgtggctgg cgttggatgt gaactcctgc tcgcgccgga
      601 tcatctgctc gatgcgcgcc tggtagtcct cgaaggcgcg cgcgagcatc gagacttccg
      661 ggtcctggcc ggggcggacg agggccgcct gctgctggcc gggtttaagc atgccgacct
      721 gatcggcgag ctccgtgacc tggaggacca gcacgcccga gagccagtag ccgaacacca
      781 gggaggcggc cgcaccggtc aggacggaaa gcagcgtgag cagcttgaac tcctgctccc
      841 gctgctcgtg caagccgacc tcgtaggcca cgatgtagcg cgtcgcgccg gcctcgcgca
      901 cgagcacgtg gtactcgtct ccgccgatga agaactcgtg ccgtcccggc gcgagcccgg
      961 tcaggaacgc gggaagctgc gcctcactcg acgccccact caggatgtag ctctggagat
     1021 tcgaactctg ctgcggctgg taaccgggat cctgccggtg gcggcggacg aggtaatcca
     1081 tttcctccag gacgagctgg tcgatcagcg actcctccat ctcctcggag gcggtgtaga
     1141 gtgtgaagcc gagcgcgccc accaccagca ggcaggcgag ggagagcgca agcgccaccc
     1201 gcagccggag tccgaactta atctccgcca cgcggcacca ggcgataacc gaggccgtgc
     1261 acgttctcga tcggatcgta gccgccggcc tgaacgagct cgcgccgcaa cacgtgcata
     1321 tggctgcgca gcgtgtcgct ggtttcctgt tgttcgcccc acacctccgc ctcgagctcc
     1381 ttgcggctga atacgcggtt gggctgcgcc atcagcacct gcaggatgcg gatgcatttg
     1441 ggcgggagcc ggacctcttt gccgcggaag cgcgccgtca ggcccgccgg atcgaaaacc
     1501 aggtcaccgg cggtgagcgg ccgggcagtg acgcgcccgg cgcggcgcct gtgcagcgcc
     1561 gcgagacgcg cttcaacctc cttgagggcg aagggcttga cgagatagtc gtcggctccc
     1621 agcgcaaagc ccccgagctt gtcttccagg gtgtcgcgcg ccgtcagcat cagcaccggc
     1681 gtgtcgtcgt gcgcctcttc gcgcagcttg cggcagaccg tggtgccatc catgcccggc
     1741 aggccgaggt cgaggatgat ggcgtcgaac ttgccggtga ccgcgagatg caacccggtt
     1801 acgccgtcgg ccgcggcatc caccacgtgc ccgccggcct cgaggaagtc gtagaggttg
     1861 gcggcgatcg ccggatcgtc ttcgatgatg agaacgcgca caggagtccg ttactcgttg
     1921 ctcgttattc gttactcgtt attcgatgac ttcgtcggcg ggaacagcaa actacgagaa
     1981 acgagcaacg aaaaacgagt cacgctttac gaggagggat gaccggctgc cagcgtccgc
     2041 tgaccagtcc ctgcatcggc tggaagtgga tcttgtaggc catcttgcga ctttgcgcga
     2101 tccagtagcc gaggtagaga taggggaggt cgagcctgcg gcaaagcccg atctgccaca
     2161 ggatgttgta ggtgccgtaa ctggcgccgc gcacgtccgg gtcgaagaag gtgtaaaccg
     2221 aggacagccc gtcctgcagc tcgtccacga tggaaaccac gcgcaacgcg c
//
LOCUS       DBNGWZ010000178         8759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1026098, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000178 DBNGWZ010000000
VERSION     DBNGWZ010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1026098"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="metG"
                     /locus_tag="AC16CX_05325"
     CDS             <1..312
                     /gene="metG"
                     /locus_tag="AC16CX_05325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR3011967.1"
                     /translation="DDFNKVDLRVAKIVNAEPVEGADKLLRLTLDLGDGTRTVLAGIK
                     TAYDPEKLKGRLTVVVANLAPRRMKFGVSEGMVLAASGEGPGLFLLSPDSGAQPGMKV
                     K"
     gene            376..1542
                     /locus_tag="AC16CX_05330"
     CDS             376..1542
                     /locus_tag="AC16CX_05330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3011968.1"
                     /translation="MQAASNRTFICSVVFLDIVEYSKKPVAEQIHFKERLNTLLTEAL
                     ANVAPNDRIILDTGDGAALSFLGDPEDALFVSLSLRDALVGPQPPGPEMKMRIGINLG
                     PVKLIRDINGQPNIIGDGINVAQRVMSFAGPGQILVSRSYYEVVSCMSEEYGHLFHYE
                     GSRTDKHVREHEVYAVQATGTTLQRAAASSSKNRAASEGRPVVLDKLSQSASFVTTSL
                     RRKPRLGTALAVTAILAVAVGLRASRPTPATPVAAPSPTSPPAKVATAPAPVPEKEAP
                     KATAPEKPATVTPKAAPAPAKPAAAPKTKKPPVTDTPPKVAARAPAMINFGISPWGEV
                     YIDGRKRGIAPPLRDVEIEPGQHRVEVRNADFPPYMEVVDAKPGSRIRIRHKFR"
     gene            1556..1957
                     /locus_tag="AC16CX_05335"
     CDS             1556..1957
                     /locus_tag="AC16CX_05335"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HIR3011969.1"
                     /translation="MNGLRLTLVAAFAVVLAGCESAPIKGIQEGFSSLFQKNKGGDDL
                     DAGIRSYENGNYKESAQQLQTALDAGLNNPNQVRAHKYLAFIHCQAGRERQCREEFRT
                     ALDIDPSFELSAAEAGHPIWGPVFRSEKNRR"
     gene            2090..3295
                     /locus_tag="AC16CX_05340"
     CDS             2090..3295
                     /locus_tag="AC16CX_05340"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3011970.1"
                     /translation="MGTRKLGRYEILAELGKGAMGVVYRANDPLLNRTVAIKTVNMSE
                     DPQELAEYEARFYQEAKAAGGLNHPNIVTIYDIGKSANVAYMAMELLEGDELRVLLSS
                     GRPLDVSRAVEIAAQVAEGLAYAHQHGVVHRDVKPANIMILRNGMAKITDFGIARMRA
                     AQVRTQTGVVLGSPRYMSPEQVAGKRADPRSDIFSLGAILYETLTGKPAFTGDDVTSV
                     MFQIINVLPPPPSSLNAAVPPVLDFIVAKALTKTPDDRYQDAGELARDLRDSAKHVDA
                     AAQAPAAARPSAQPTLDTEAAQALARSFPDERSADAGSAATNSGPALGLSKEFDSLAA
                     TSKLAAQIGMSEALKDFPQAATSSSAISPSAMVAAMRRGPGWSAKDKIIFAASVTGAL
                     VIGAIIVLV"
     gene            3420..4529
                     /locus_tag="AC16CX_05345"
     CDS             3420..4529
                     /locus_tag="AC16CX_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3011971.1"
                     /translation="MKIESIEAIAIEIPLKKNFGGSTYSVLKRCTVITRARTDEGLTS
                     EVYNGDNRENGHEIVRLIQEDLAPRVKGSDILEVERAWQAMFDLTHTSRDRKTLLEAV
                     SCVDCALWDLVGKARGKSVSALLGGNRDRLPIISIGGYYMEGKTLADIGREMESYRAA
                     GMAGCKFKVGGLAPDEDAKRVEAARKAAGPDFMLCVDANRGWKAQDAVRFARRVEHLD
                     IGWFEEPCHWYDDVALMARVRQATRIPVTAGQCEITSHGVRRLVDAGAVDFVNFDVSE
                     GGGVTDWRRAAEICRTAGVRMAHHEESQISQQLLAGVPHGTYVECFADPDRDPVWQSV
                     WANRPPVRNGQMDVSKEPGFGIRLDWKLVERYRIA"
     gene            complement(4564..5160)
                     /locus_tag="AC16CX_05350"
     CDS             complement(4564..5160)
                     /locus_tag="AC16CX_05350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3011972.1"
                     /translation="MFDLGGVVVEWEPDAIIARVFPDPAIQALARSEIIGHADWLALD
                     RGTLTQQDAIARAAGRTGVPEAGVAELLRQVPPALVAIPGTVDLLYRLKARGHTMFCL
                     SNIQAPSIEHLEKAYDFWEVFKGVVVSCRLHLCKPEPAIYSHLLRTYGLVGADTVFID
                     DTEANLTAAASFGMHTIRFENPAQCERRLQALGCIGEP"
     gene            complement(5211..6257)
                     /locus_tag="AC16CX_05355"
     CDS             complement(5211..6257)
                     /locus_tag="AC16CX_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3011973.1"
                     /translation="MRAMVLEQFGAPLKLKDMAVPKIGPNDALVKVRVCGVGLTVVIM
                     IATPGRIKSFPRIPGHEIAGEVVEVGSEVRTVKVGQRVTNHFYLTCGQCRYCRSGRET
                     LCENFRGNVGSGCDGGYAEYVALPERNLVAIPDGVTDLEASVASDAIATPYHACHAEA
                     QVSPGDNVLIIGAGGGVGIHMLQMARLCGGRVLAAEIGEEKLSLAREMGADEIIDARR
                     GDLVEQVKTLTGGRGVDATIDMVGNSETLQASLATLAPGGRMVIIGSKPKSVYGKDPS
                     FVVNPQEFMRQAQEIHGSRYVNNSEIIRTLELVKQKRIKAVVNKTFPLEDAEKVHELL
                     RKNAIAGRAALIVS"
     gene            complement(6388..6903)
                     /locus_tag="AC16CX_05360"
     CDS             complement(6388..6903)
                     /locus_tag="AC16CX_05360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR3011974.1"
                     /translation="MYQLLKQIAVTGIKTEAPPAADEALRVTADRLRADILEYFNGAL
                     TIRHVDAGSCNGCELEIHMCNNPHYNLEGLGIKFVASPRHADMLLVTGPVSRHMEVAL
                     KRTYDATPDPKLVVAIGDCGCTGGIFGESYASRGRVSNVIPVDVAVPGCPPTPLAIMQ
                     GILTAITAKKA"
     gene            complement(7024..8583)
                     /locus_tag="AC16CX_05365"
     CDS             complement(7024..8583)
                     /locus_tag="AC16CX_05365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR3011975.1"
                     /translation="MRLEGLNIELTPIAGAVPVYRAEVGAAQWTETCRQVAEQGGRLV
                     ALWGTDARDEGRGFSAHAALVVRSGLIVLSLPLSATSYPDVSAIFPTADRMQRAALDL
                     LGISAEGADDRRKWLRHTAWRADEYPLRKDFRAGNESPPGRHAVNEADDYPFVRVEGE
                     GVHEIPVGPVHAGTIEPGHFRFSVVGETVLRLEERLGYKHKGIEKRFEQMTITEGARL
                     AGRVSGDSTVAYAWAYAMAVESAAGVTPPPRALWLRALLLERERVANHLGDLGFLGND
                     AALAFGLAQFLRLKEEWLRTGQAAFGHRYLMDAIVPGGVARDATPGGMVLMRRELDAL
                     EKTVAVLKSVYDEHAGLQDRFINCGRVAPRLAARLGLIGVAGRASGQAWDLRVEYPCI
                     PYPELDVRMATHRNGDVAARVTVRFEEIIESLRLLRLILDRLPLGEPAVAVPAPAAGA
                     FGVGWVEGWRGEVFIALEAGADGRIRRCHPHDPSWQNWPVLEHAVIGNIVPDFPLINK
                     SFNLSYSGHDL"
BASE COUNT         1573 a         2998 c         2716 g         1472 t
ORIGIN      
        1 gacgacttca acaaggtgga cctccgcgtc gcgaagatcg tgaacgcgga acccgtggaa
       61 ggcgcggaca agctcttgag gctcacgctc gatctgggtg acggaaccag gaccgtgtta
      121 gccggcatca aaacagccta tgatcccgaa aagctgaaag gcaggcttac cgtcgtggtc
      181 gccaacctcg ccccgcgcag gatgaagttc ggcgtctccg agggcatggt gctcgctgcg
      241 agcggcgagg ggccgggctt gtttctgctc tcccccgact ccggcgccca gcccgggatg
      301 aaggtgaagt agcgcctcgg gccagcttct catcacccat cactcatcac ccatcacgat
      361 ttcagagcga aggcgatgca agcagcaagc aaccgcacct tcatctgcag cgtcgtgttt
      421 ctcgacatcg tcgagtactc gaagaagccg gtcgccgagc agatccactt caaggagcgg
      481 ctgaacaccc tgctcaccga ggcgctcgcg aacgtcgcgc ccaacgaccg catcatcctc
      541 gacaccggcg acggcgcggc gttgagcttc ctcggcgatc cggaggacgc gctgttcgtg
      601 agcctctcgt tgcgcgacgc gctcgtgggc ccgcagccgc cggggccgga gatgaagatg
      661 cgcatcggca tcaacctcgg cccggtcaag ctgatccggg acatcaacgg ccagcccaac
      721 atcatcggcg atggcatcaa cgtcgcgcag cgtgtgatga gcttcgccgg gcccggccag
      781 atcctggtgt cgcggtccta ttacgaggtg gtgtcctgca tgtccgagga gtacggccat
      841 ctcttccatt acgagggctc gcgcaccgac aagcacgtgc gcgagcatga ggtgtacgcg
      901 gtgcaggcga ccggtacgac tttgcagcgc gccgccgctt ccagctccaa aaaccgggcg
      961 gcttccgagg gccggccggt cgtgctcgac aaactgtcgc aatccgcgtc ctttgtcacg
     1021 accagcctgc gccgcaagcc gcggctcggc accgcgctcg cggtcaccgc catacttgct
     1081 gttgccgtgg ggctgcgggc aagccgtccg acgcccgcca cgcccgtggc ggcgccttcc
     1141 ccgacttcgc cgccggccaa ggtcgccacc gctccggctc ccgtccccga gaaggaagca
     1201 cccaaagcga cggctcccga aaaacctgcc actgtcacgc ccaaggccgc ccccgctccg
     1261 gcaaagcccg ccgccgcccc aaaaaccaag aagcctcccg tgactgatac cccgcccaag
     1321 gtagcggcga gagcgcctgc catgatcaac ttcgggatct cgccctgggg cgaggtttac
     1381 atagacggca ggaaacgggg tatcgcgccc cccctgcgcg acgtagagat cgagccgggc
     1441 cagcatagag tcgaggtcag aaacgccgat tttccgccct acatggaagt tgttgatgcg
     1501 aaacccggaa gccggattag aattaggcat aagttccgct gacgggagga ttaccatgaa
     1561 cggtttgcga ttaacgctgg tggcggcgtt cgccgtcgtc ctggccggat gcgagtccgc
     1621 gcccatcaag gggatccagg aaggcttcag cagtctgttc cagaagaaca agggcggaga
     1681 cgacctcgac gcgggcatac gctcgtacga aaacggcaac tacaaagagt cggcacagca
     1741 gcttcagacc gcgcttgacg ccggactgaa caacccgaac caggtgaggg ctcacaagta
     1801 cctggccttc atacactgcc aggccgggcg tgaacggcag tgccgtgaag agttccgaac
     1861 ggcgcttgat atcgacccct cgttcgagct cagcgccgca gaggccgggc acccgatctg
     1921 ggggccggta ttccgcagcg agaagaaccg ccggtgaaag gcaaatggac aggatttaca
     1981 tgatcaacag gatcaaaccc cgatcaaatt caagacggtc gttcggggct gattgttgtt
     2041 ggttttaatc ttgtaaatcc tgttaatcct gtctattcca gcttttgtca tgggaacgcg
     2101 caaactcgga cgctacgaga tcctcgccga gctcggcaag ggcgcgatgg gggtcgtcta
     2161 tcgcgcgaac gatcccctgc tcaaccggac ggtcgccatc aagaccgtca acatgtcgga
     2221 ggatccgcag gagctggcgg agtacgaggc gcgcttctac caggaagcca aggccgccgg
     2281 cgggctcaac catcccaaca tcgtcaccat ttacgacatc ggcaagagcg ccaacgtcgc
     2341 ctacatggcg atggagcttc ttgaaggcga cgagctgcgc gtcctgctgt cgtcaggcag
     2401 gccgcttgac gtttcgcgcg cggtcgagat cgccgcccag gtggcggaag ggctcgccta
     2461 cgcccaccag cacggcgtcg tgcaccgcga cgtgaagccc gccaacatca tgatcctgcg
     2521 caacgggatg gcgaaaatca ccgacttcgg catcgcgcgc atgcgtgcgg cccaggtgcg
     2581 cacgcagacc ggcgtcgtgc tcggctcgcc gcgctacatg tcgcccgagc aggtcgcggg
     2641 taagcgcgcc gatccgcggt cggacatctt ctcccttggc gcgatcctct acgaaacgct
     2701 cacgggcaag cctgcgttca ccggcgatga cgtgacttcg gtgatgttcc agatcatcaa
     2761 cgtgttgccg ccgccgccaa gctctctcaa tgcggcggtt ccccctgtgc tggatttcat
     2821 cgtcgccaag gcgctcacca agacgcccga cgatcgctat caggatgccg gcgaactcgc
     2881 gcgtgacctg cgcgacagcg ccaaacacgt ggacgccgct gcacaggcgc ctgcagctgc
     2941 gcgaccctcc gcgcagccga cgctcgatac ggaggcggcg caggcactgg cgcgctcgtt
     3001 tccggacgag cgcagcgccg atgccgggag cgctgctaca aactctggtc cagctctggg
     3061 cctgtccaag gagttcgatt ccctggcggc cacttccaag ctcgctgccc aaattggaat
     3121 gtcggaggca ctcaaggatt tcccgcaggc cgccacttcc agcagcgcga tctcaccgag
     3181 cgccatggtc gccgcgatgc gccgggggcc gggctggagc gcgaaagaca agatcatttt
     3241 cgcggccagc gtgacagggg ccttggtcat cggcgcgata atcgtcctcg tctaaaaggc
     3301 gggactgggg actcgggacc aggggctcga aaatacaagc ttccagcccc gaaccccgaa
     3361 tgccaagtcc cgctcttcgc tcttggtcaa gccccgagac ccgatagccg agccccgctt
     3421 tgaaaatcga gagcatcgaa gcaatcgcga tcgaaattcc tctgaagaaa aacttcggcg
     3481 gctcgactta ctccgtcctc aaacgctgca ccgtgatcac ccgcgcgcgc acggatgagg
     3541 gactcacgag cgaggtctac aacggggaca accgcgagaa cggacacgag atcgtccgcc
     3601 tgatccagga ggacctggcg ccgcgcgtca aggggtcgga catactcgag gtcgagcgcg
     3661 cctggcaggc gatgttcgac ctcacccaca cgagccgcga ccgcaagacg ctgctcgagg
     3721 cggtctcgtg cgtggactgc gcgctgtggg acctcgtcgg caaggcgcgg ggaaaaagcg
     3781 tcagcgccct cctcggcggg aaccgcgacc gtctccccat catctccatc gggggctact
     3841 acatggaggg caagacgctc gccgacatcg ggcgcgagat ggagtcgtac cgcgcggccg
     3901 gcatggcggg ctgcaagttc aaggtcggcg gcctcgcgcc cgacgaagat gcgaaacggg
     3961 tcgaagccgc gcgcaaggcg gcgggccccg atttcatgct gtgcgtggac gccaaccgcg
     4021 gctggaaggc gcaggacgcg gtccgcttcg cgcggcgcgt cgagcatctg gatatcggct
     4081 ggttcgagga gccgtgccac tggtacgacg acgtggcgct gatggcgcgc gtgcgccagg
     4141 cgacccgcat cccggtcacc gccggccagt gcgagatcac cagccatggc gtgcgccggc
     4201 tggtggacgc cggtgcggtg gatttcgtga acttcgacgt ctcggaaggc ggcggcgtca
     4261 ccgactggcg ccgcgcggcg gagatctgcc gcaccgcggg cgtgcgaatg gcgcaccacg
     4321 aggagtcgca gatttcccag cagctcctcg ccggcgtgcc gcacggcacc tacgtcgagt
     4381 gttttgccga ccccgaccgc gacccggtgt ggcagtcggt gtgggccaac cgcccgccgg
     4441 tcaggaacgg ccaaatggat gtgtcaaagg agccggggtt cggcatccgg ctcgactgga
     4501 agctggtgga gcgctaccgc atagcctgaa ccacgtgact cgtgactggt gactcgtgac
     4561 tgatcacggc tcgccgatgc aacccagtgc ctgcagccgg cgctcgcatt gcgccgggtt
     4621 ttcgaaccgg atggtgtgca tgccgaagct ggcagcggcg gtcaggttcg cttccgtgtc
     4681 atcgatgaat accgtgtccg ccccgaccag accgtacgtc cgcaggagat ggctgtagat
     4741 ggcgggttcc ggcttgcaca gatgcaggcg acaggaaacg acgaccccct tgaagacctc
     4801 ccagaagtcg tacgcctttt ccagatgctc gatggagggc gcctgtatgt tcgacaggca
     4861 gaacatcgtg tgtccccgcg ccttcagtcg atacagcagg tccacggtcc ccggaatcgc
     4921 gaccaacgcg ggcggtacct gccgcagcag ctccgccacg ccggcttccg gaacgccggt
     4981 tcgcccggcg gcgcgcgcaa tggcgtcttg ctgggtaagc gtgccgcgat cgagcgcgag
     5041 ccagtcagca tggccgatga tctccgagcg cgccagcgcc tggatggccg gatcggggaa
     5101 taccctggcg atgatggcat cgggctccca ttccaccacg accccaccca gatcaaacac
     5161 gatgttcaag acgacctttc acgaccgcgc cgcccgccgc agcaagcggt tcagctgaca
     5221 atcagcgccg cgcgcccggc aatcgcgttc ttccgcagca gctcatgcac cttctccgcg
     5281 tcctccagcg ggaaggtctt gttcaccacc gccttgatcc gcttctgctt caccagctcc
     5341 agcgtgcgga tgatctcgga attgttcacg tagcgcgagc cgtgtatctc ctgcgcctgc
     5401 cgcatgaact cctgagggtt caccacgaac gacggatctt tgccatacac ggatttgggc
     5461 ttcgagccga tgatcaccat gcggccgccg ggcgcgagcg ttgcgaggct cgcctgcagc
     5521 gtctcgctgt tgccgaccat gtcgatcgtc gcgtccacgc cccgcccgcc cgtgagcgtc
     5581 ttcacctgct ccacgaggtc cccgcgccgc gcgtcgataa tctcgtccgc acccatctcg
     5641 cgcgccagtg acagtttttc ctcgccgatc tcggcggcga gcacccggcc gccgcagagc
     5701 cgcgccatct gcagcatgtg tatgccgacg ccaccgccgg cgccgataat gagcacgttg
     5761 tcaccagggc tcacctgcgc ctccgcgtgg caggcgtggt acggcgtcgc gatcgcgtcg
     5821 ctcgccaccg atgcctcgag gtccgtcacg ccgtcgggaa tcgcaaccag gttgcgctcc
     5881 ggcagcgcca cgtattccgc atagccgccg tcgcagccgc tgccgacgtt gccgcggaaa
     5941 ttctcgcaca gcgtttcgcg cccgctgcgg cagtagcgac actggccgca ggtgagatag
     6001 aaatggttgg tcacgcgctg accgactttg actgtcctca cttccgaacc cacttccacc
     6061 acttcgccgg caatctcgtg cccgggtatg cgcgggaacg acttgatccg ccccggcgtc
     6121 gcaatcatga tcacgaccgt gaggccgacg ccgcagacgc gcaccttgac cagcgcgtcg
     6181 ttcgggccga tcttcggcac cgccatgtcc ttgagcttca acggcgcgcc gaattgctcc
     6241 agcaccatcg ctcgcatcgt ttcctcccaa ctgcgtgata ggtgataggt gataggagct
     6301 tacaacccgc tccgcgggag attttccctt atctcttatc tcttatctct tatctcttat
     6361 ctcttatcac ttatcactca tcacttatca cgctttcttc gcggttatcg cggtgaggat
     6421 gccctgcatg atcgcgagcg gcgtcggcgg gcatcccggc actgcgacat ccaccggaat
     6481 cacgttcgac acccgcccgc ggctcgcgta gctctcgccg aagatgccgc cggtgcagcc
     6541 gcaatcgccg atcgcgacca ccagcttcgg atcgggcgtc gcatcgtagg tgcgcttgag
     6601 cgccacttcc atgtggcgcg acaccgggcc cgtcaccagc agcatgtcgg cgtgacgcgg
     6661 gctcgcgacg aacttgatgc ccagcccttc caggttgtag tgcgggttgt tgcacatgtg
     6721 gatctcgagc tcgcaaccgt tgcacgagcc ggcgtccacg tggcggatgg tgagcgcgcc
     6781 gttgaagtac tcgagaatgt ccgcgcgcag cctgtccgcc gttacgcgca gcgcctcatc
     6841 ggccgccggc ggcgcttcgg tcttgatccc cgttacagcg atctgcttca gcagctgata
     6901 cacggcttac accctgtaaa tgggaaatag gaaataggaa atggaaaaat cattcggcta
     6961 cgaaccttac cccttcccgt tttcggtttc cattccccat ttcccattcc ccattccctt
     7021 tgctcacaag tcgtggccgc tgtacgacag gttgaacgac ttgttgatga gcgggaagtc
     7081 gggaacgatg ttgccgatca ccgcgtgctc gagcaccggc cagttctgcc acgacgggtc
     7141 gtgcggatgg cagcgccgga tgcggccgtc cgccccggcc tcgagcgcga tgaagacttc
     7201 gccgcgccag ccttcgaccc agcccacgcc gaatgcgccc gccgcgggcg ccgggaccgc
     7261 gaccgcgggt tcgcccaacg gcaggcgatc gaggatgagg cggagcagcc gcagcgactc
     7321 gattatttcc tcgaagcgca ccgtgacgcg cgcggcgacg tcgccgttcc ggtgcgtggc
     7381 catgcgcacg tcgagctcgg gatacgggat gcacgggtac tcgacgcgca ggtcccatgc
     7441 ctgcccgctc gcgcgccccg cgacgccgat cagcccgagg cgggccgcga gcctgggcgc
     7501 gacccgcccg cagttgatga agcggtcctg caagccggca tgctcgtcgt aaacggattt
     7561 gagcaccgcc accgtttttt ccagcgcatc caactcccgc cgcatcagca ccatgccccc
     7621 gggcgtcgcg tcgcgcgcca ccccgccggg aacgatcgca tccatgaggt agcggtggcc
     7681 gaacgccgcc tggccggtcc gcagccattc ctccttcagg cgcagaaact gggcgagccc
     7741 gaaggcgagt gccgcgtcgt tgccgaggaa cccgagatcg ccgaggtgat ttgcgacgcg
     7801 ctcgcgctcg agcagcaacg cccgcagcca cagcgcgcgc ggcggcggcg tcaccccggc
     7861 ggccgattcc accgccatcg cgtaggccca tgcgtaggcc acggtcgaat cacccgacac
     7921 gcgccccgcg agcctggcgc cttccgtgat cgtcatttgc tcgaagcgct tctcgatgcc
     7981 cttgtgcttg tagccgaggc gctcctccag ccgcagcacc gtttcgccga cgacggagaa
     8041 gcggaagtgg ccaggctcga tggtgccggc gtgcaccggg ccgacgggaa tctcgtggac
     8101 accctctccc tcgaccctaa cgaacggata gtcgtccgct tcattaacgg cgtggcgccc
     8161 gggcggtgat tcgttgccag cgcgaaaatc tttccggagc ggatattcgt ccgctcgcca
     8221 cgccgtatgg cgcagccatt ttcggcggtc gtcggcgcct tccgccgaaa tgcccagcag
     8281 gtcgagggcc gcgcgctgca tgcggtccgc cgtcgggaag atcgcgctca catcggggta
     8341 cgacgtagcg gagagcggga gcgacagcac gatgagccca ctgcgcacca cgagcgcggc
     8401 atgggcggaa aacccgcgcc cctcgtcgcg cgcgtcggtt ccccacaacg cgaccagccg
     8461 tccgccctgc tcggctacct ggcggcaggt ttcggtccac tgcgcggcgc cgacctcggc
     8521 gcggtacacc ggcaccgccc cggcgatcgg cgtcaattcg atattgagcc cttccagcct
     8581 catcctttcg ccttccgact ttcgactttc gccttagcca atcagcgccg ccgcctgccg
     8641 gtaccacgcc gcaagatacg gcgggatgaa gatccccagc atcagtacca gcgccagatg
     8701 cacgaacacc gggatcagcg ccggcgggtg cggcagccgc ggcaactgcg tctcgccga
//
LOCUS       DBNGWZ010000179         3718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2285865, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000179 DBNGWZ010000000
VERSION     DBNGWZ010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2285865"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="AC16CX_05370"
     CDS             <1..659
                     /locus_tag="AC16CX_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011976.1"
                     /translation="WSPQWLDVFAKVRIRPRKTYHPGATRTFGQAPKQVALCDVREFV
                     ERFQRTLRTAKPKPPAPPRRNAHAGEASEAPALVPPPSQAEVAGLRQENQELRAGVAR
                     LGQQLGAARAAVEALRRVLEPEYRAMQKLFSDLDAAGANTGGIDAAVFEPWKEKVGTG
                     PARMLEALIQRNGELTRHQLSTLSGYSVGSSTFREYLGRLSRNGLIVREGERIRLRVP
                     "
     gene            694..1167
                     /locus_tag="AC16CX_05375"
     CDS             694..1167
                     /locus_tag="AC16CX_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011977.1"
                     /translation="MTGDMQRETRELAVAIYGLVSETRSMLFRTVIVVVLLVVFLALL
                     AVVASAQTPTQVTGTIKMPNGQAVAGRIEFELSAPGRVADPALLLVQPRVSCAITAGA
                     IAAGCALRGNDTITDPAGTYYRVRILAADGHELMKWKKYSITGATWDIGTHTPLP"
     gene            1178..>3718
                     /locus_tag="AC16CX_05380"
     CDS             1178..>3718
                     /locus_tag="AC16CX_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011978.1"
                     /translation="MEERMKKRILLAALALLIAAAGAEATTVTGTVKDASGAPLASGT
                     ITFTLSQEATSTEPALYLAATVTCAITSGAIQSCTVEANDLLSPGGTYYKVRILGAYK
                     QEILPERNYTITGATWDLGSHPPLASAPVAAKAYQVIQDEGLPLPQRATANFTGAGVA
                     CSDDPTNARTNCAISGGGGGGATHQVNGVALAAQDPINFQDSTTIAVTNPSLGNVQLG
                     VKNSSVTDAMLASTLHLNTATFGRLNGERFADGTRNATLAAAIADCVANSCLAGDALE
                     GGQSIAANLFAGITGAGKLSFAPVTYAVTATSTPPVNWLLACSSGFQGSWWPPSSGQT
                     IFSNTAASTPTFILGSTSVTGCNFLGSGAGAHLNVTGEGVRVADSYIGGKQNAILIDN
                     SGGNAYNGLFERLLLTGTSDLIKANSPSGAIPNLHFRDIWFRKDDAGLRSGKGFSMTG
                     AAAGMRAFDFTSDTFSGFTDTGSGVALDLYGCGGGSISNSLFEGFSGVGSDIVFLGNC
                     TGPVFMGPNVFDTYYSAINLGSVAGAFIVPQSFIGGDPAGYDYIINGGAGGARDVFIF
                     GANAARTFGIGEREHVFFSNGTDLFHNRTGYKLAAMGDPSYGVGFSWNRQVTGSVMEK
                     WFAGLAADATEDWLLNNATGANFVRAKQDGSRLEINKPLQITGGLTLAGTGGTGQFLK
                     QKTLGGAITVETIAAGDLPAHAHAESDVTSLVADLAAKEATANKNAANGYAGLNASAQ
                     IAIGRTPLTTRGDLLGVDLTPALVRIAIGVAGTCLTSNGTDASWGSCGGAHDLLSATH
                     SDTTAGVVARGDVITGQGVTPKWARLAKGLAGQCLQMDGSAVDVTWGSC"
BASE COUNT          705 a         1075 c         1329 g          609 t
ORIGIN      
        1 tctggtcgcc gcaatggctc gacgtctttg ccaaggtgag gatccggccg cgcaagactt
       61 atcacccggg ggcgacgcgg accttcgggc aggcgccgaa gcaggttgcc ctgtgcgatg
      121 tccgggagtt cgtcgagcgg tttcagcgga cgttgaggac cgcaaaaccg aagccgccgg
      181 ctccgccgag gaggaacgct catgcgggcg aggcgtcgga ggcgccggcg ctggtacctc
      241 cgcccagcca ggcggaagtc gccgggctgc ggcaggagaa tcaggagttg cgcgccggcg
      301 tggcgcgact tggccagcaa ctcggtgcgg cgagggcggc cgtagaagca ctgcggcgcg
      361 tgcttgagcc ggaataccgg gcgatgcaga aactcttcag tgacctggac gcggccgggg
      421 cgaacaccgg cgggatcgat gccgcggtct tcgaaccgtg gaaggagaaa gtgggaaccg
      481 ggccggccag gatgctggag gcgctgatcc agcgcaacgg cgagttgact cggcaccaac
      541 tctcgacctt gtcgggttac agcgtcggct cgagcacgtt ccgggaatac ctcgggagac
      601 tgagccggaa cggtctgatt gtgagggagg gcgagcgaat ccgcctgcgg gtaccgtgat
      661 ggagcagacc ggaccagcgc cgaaggagga ggaatgacgg gcgatatgca gagagagacg
      721 agggaactgg cggttgcgat ttatggcctg gtgagcgaaa cgcgctcgat gctcttccgc
      781 actgtcatcg tggtggttct gctggtggtt ttcttggcgc tcctggcggt ggtggccagt
      841 gcgcagacgc cgacgcaggt gacgggtacg atcaagatgc cgaatgggca ggcagtggcc
      901 gggagaatcg agtttgaact ttcagcgccg ggacgcgtgg ccgatccggc gctgctgttg
      961 gtccagccgc gagtgagttg cgcgatcacc gcgggcgcga tcgcggccgg gtgcgcgttg
     1021 cgcgggaacg acacgatcac cgacccagcg ggaacgtact accgagtgcg gatcctggcg
     1081 gccgacggtc acgaactgat gaagtggaag aagtattcga tcaccggggc aacctgggac
     1141 atcggcaccc acaccccgct gccgtaatca gaggacgatg gaggaacgga tgaagaaaag
     1201 gattctgctg gccgcgctgg cgctgctgat tgcagcggcg ggcgccgagg cgacgaccgt
     1261 gaccggcacg gtgaaggatg cgagcggggc gccgctggcc tcgggcacga tcaccttcac
     1321 actgtcgcag gaggcgacct cgaccgagcc ggcgctttat ctggcggcga cggtgacttg
     1381 cgccatcacg agcggcgcga tccagagttg cacggtcgaa gcgaacgacc tgctctcgcc
     1441 cggcggcact tactacaagg tgcgcatcct gggcgcctac aagcaggaaa tcctgccgga
     1501 gcggaactat acgatcaccg gggcgacgtg ggacctcggg tcgcatccgc cgcttgccag
     1561 cgcgccggtg gcagcgaagg cttatcaggt catccaggac gagggcctgc cactgccgca
     1621 acgggccacc gcgaatttta ccggcgcagg agtggcctgc tctgatgacc cgacaaacgc
     1681 gcgaacgaat tgcgcgatct cggggggcgg cggcggcggc gcaacgcatc aggtgaacgg
     1741 cgtggcgctc gcggcccagg atccgatcaa cttccaggac tcgacgacga ttgcggtcac
     1801 gaacccgagc ctcggcaatg tccagctcgg agtgaagaac agctcggtca cggatgcgat
     1861 gctcgcctcg acgctgcatc tgaacacggc gaccttcggg cggctgaatg gggaaagatt
     1921 cgcggacggg acgaggaatg caacgctggc tgcggcgatt gccgactgcg tagcgaacag
     1981 ttgcctggct ggcgatgcgc tcgagggtgg ccaatccatt gcagccaacc ttttcgccgg
     2041 gatcaccgga gccgggaaac tgtccttcgc gccggtgacc tatgcggtga cagcgacgtc
     2101 cacgccgccg gtaaattggc tgctggcgtg ttcgtcaggt tttcagggca gttggtggcc
     2161 gccgagttcg ggccagacaa tcttctcgaa cacggcggca agtacgccca cgttcatcct
     2221 ggggtcgacg tcggtcaccg gatgcaactt cctgggaagc ggggcggggg cgcacctgaa
     2281 tgtgacgggc gagggcgttc gggtcgccga ctcctatatc ggtgggaaac agaacgccat
     2341 cctgatcgac aacagcggag gtaacgccta caacgggttg ttcgagaggt tgctgctcac
     2401 cgggacaagt gacctgatca aggcgaactc gccttcggga gcgatcccca atctgcactt
     2461 ccgggacatc tggttccgca aggatgatgc gggcctgcga tcagggaagg gcttttcgat
     2521 gaccggagcg gccgcgggga tgcgggcgtt cgattttacc tcagatacct tcagtggctt
     2581 cacggacacc ggttcgggcg tcgccctgga cctctacgga tgcggcgggg gcagcatctc
     2641 gaacagtctg ttcgagggtt tcagcggcgt gggttcggac atcgtttttc tcggcaactg
     2701 caccggaccc gtgttcatgg ggccgaacgt cttcgacacc tactacagcg cgatcaatct
     2761 gggcagcgtc gccggcgcat tcatcgtgcc gcagtccttc atcggtggag acccggccgg
     2821 ctacgactac atcatcaatg gcggtgccgg gggcgcgcgg gatgtgttca ttttcggtgc
     2881 gaatgcggcg cgcacattcg ggatcggaga acgggagcac gtctttttca gtaacggcac
     2941 ggacctcttc cacaaccgaa ccgggtacaa actggccgcg atgggcgatc ctagttacgg
     3001 ggtgggtttc tcctggaacc ggcaggtaac cggcagcgtg atggagaagt ggttcgcagg
     3061 gcttgcagcc gatgcgaccg aggactggct gctcaacaat gctacgggtg cgaacttcgt
     3121 tcgcgcaaag caggacggca gccggctgga aatcaacaaa ccgcttcaga tcaccggagg
     3181 tttgacgctg gccgggacgg gaggcaccgg ccagttcttg aagcaaaaaa cgctcggcgg
     3241 cgcgatcaca gtcgagacaa tcgctgccgg cgacctaccg gcacacgcgc acgcggaaag
     3301 cgacgtcacc agtctggtgg ccgacctggc ggcaaaagaa gcgacggcaa acaagaacgc
     3361 ggcgaacggt tacgccggcc tgaatgccag cgcgcaaatc gcgatcgggc ggacgccgtt
     3421 gacgacgcgc ggcgatttac tgggggtgga cttgaccccg gccctggtgc ggattgcgat
     3481 tggggtggcg gggacgtgcc tgacgagcaa cggtacggat gcgtcttggg gttcctgcgg
     3541 cggcgcgcac gacttgctct cggcgaccca tagcgacacg accgccggag tagtggcccg
     3601 cggcgacgtg atcaccgggc aaggcgtgac gccgaagtgg gcgcgcttgg cgaaagggct
     3661 ggctggacaa tgcctgcaga tggatggcag tgcggtggac gtcacctggg gatcatgc
//
LOCUS       DBNGWZ010000180         4682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1906547, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000180 DBNGWZ010000000
VERSION     DBNGWZ010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1906547"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1100)
                     /locus_tag="AC16CX_05385"
     CDS             complement(315..1100)
                     /locus_tag="AC16CX_05385"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3011979.1"
                     /translation="MDIVPNHTRRRLAAGELALGMGVRQSRTVDIATAAKTCGFDWLF
                     IDMEHSSLDVDLASQLAMASLGAGITPLVRVPGHEHYHASRLLDNGAQGIVAPHVDSV
                     EEAQRIARACRYPPLGHRSVAGAQPQLGFRSVPVGEATRLVNEEMLVVVMLETPKAIA
                     GAEAIAQVEGVDVLLIGTNDLCAEMGIPGKFGDAKVEEAYRKVVAACRKHGKFPGMGG
                     VYEPKLMEKYIGLGMRFILSGNDFSFMMAGARERASFLRSVKL"
     gene            1303..2259
                     /locus_tag="AC16CX_05390"
     CDS             1303..2259
                     /locus_tag="AC16CX_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3011980.1"
                     /translation="MRFVLLLIALTAAAASVAQPYPSKPVRFLVGYAPGGATDINARI
                     VALKLTEMWGQQVVVDNRPGASGTIAAELTAKAAPDGYTLLVAPQTSVVAAPLIFRKV
                     GFDTLKDFAALSIIGSTPLLLVVHPSLPPKTFKEFTSFMKSNASRLSYATGGIGSTPH
                     FAAELLSISLGVKMVHVPYKGENPAVADIISGQIPLMFSNISAVLPFVKAGRVRALAI
                     TSPKRSPLAPGYPTVAESGFPDFDVATWAGLYAPAAINRELGARISGDIAKVIRMPDV
                     KERMANQGIDPVGSTPDEHLAYVKSEFTRIGRVAREANIRAD"
     gene            2373..4109
                     /gene="mutL"
                     /locus_tag="AC16CX_05395"
     CDS             2373..4109
                     /gene="mutL"
                     /locus_tag="AC16CX_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR3011981.1"
                     /translation="MAAIRVLPELLVSQIAAGEVVERPAAALKELLENSLDAGAREIA
                     VQLSGGGAKLVKVTDDGDGIGRDELRLALARHATSKIASLEDLERVTSLGFRGEALAS
                     IAAVSRLTLTSRRAGDKHAWTVSASGAEVTRAAPAALAAGTSVEARDLYFNTPARRKF
                     LKSDATEYAHCEEAFKRAALSRPDAGFTLQHNGRAQWHLKPQPLRERIGALLGEDFNA
                     TALALDEASAGLRLTGAIGLPAHARATREAQYFFVNGRFVRDKLAAHAIRQAYQDVLH
                     HDRHPAFVLFIEIDPGRVDVNVHPTKTEVRFRDPRGVHQFIFHALNKALAGAVAGAGQ
                     TRGTAVAPVAGIAAAPMRQAAMALNVAEPVAFYETLFRKPGPEEAAPETEDAPLGFAL
                     AQLSGIYVLAQNRHGLVIVDMHAAHERIMYEKLKRALEERGIPMQPLLVPVTFATEAL
                     DVATADEHGDVLRTIGFDIAAASPTSLIVRAVPAPLADADARRLAVDVLREIRETGAS
                     RVLTERRNELLATMACHAAVRANRKLTVPEMNALLREMEETERSGQCNHGRPTWHQLS
                     LAELDRLFMRGR"
     gene            4364..>4682
                     /locus_tag="AC16CX_05400"
     CDS             4364..>4682
                     /locus_tag="AC16CX_05400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR3011982.1"
                     /translation="MGPTASGKTVVAARLARELPCEIISVDSAQIYKGMDIGTAKPEA
                     ELLKRAPHHLLDVIEPHESYSAARFRDDALALMREITERDRIPLLVGGTMLYFRALTE
                     GLNE"
BASE COUNT          820 a         1629 c         1486 g          747 t
ORIGIN      
        1 gatctccctg ctcggcgccg caccgtatga ggacggcgaa ccgtgcgaat tataaatgaa
       61 ctaaagcgga caccatacgc gccgctttag ttgcgtgcgt tcgagcgggc ggcacaccgc
      121 cgctcgacgc ccgcctcatt gacagcaaaa aactgccggg aataatgccg gataaatccg
      181 ccgtcatgct tgctgaatct ggcattggct tgatctacat caaacggcag ctttcagcat
      241 tcagcgcccg gctttcagag tcaaacgagc acgcctcagg ctgatggttg atagctgacg
      301 gctgacagct ggcgtcatag cttgacggag cgcaggaacg acgcccgctc gcgcgcgccc
      361 gccatcatga acgagaaatc gttgccgctc aggatgaaac gcatgccgag accgatgtat
      421 ttctccatga gcttgggctc gtagacgccg cccatgccgg gaaacttgcc gtgcttgcgg
      481 catgcggcga cgactttccg gtaggcctcc tcgaccttcg cgtcgccgaa cttccccggg
      541 atgcccatct ccgcgcacaa gtcgttggtt ccgatcagca ggacatcgac gccttccacc
      601 tgcgcgatcg cttccgcgcc ggcgatcgct ttcggcgtct cgagcatgac gacgacgagc
      661 atctcctcgt tcaccaggcg cgtcgcctcg cccaccggca cgctcctgaa gccgagctgc
      721 ggctgcgcgc ccgcgaccga gcggtggccg agcggcggat agcggcaggc acgggcgatg
      781 cgttgcgctt cctcgacact gtccacgtgc ggggcgacga tgccctgcgc gccgttgtcg
      841 agcaggcgcg aggcgtggta atgctcgtgc cccggcaccc gcacgagcgg tgtgatcccg
      901 gcgccgagcg aggccatggc gagctgcgag gcgaggtcca cgtcgagcga gctgtgctcc
      961 atgtcgatga acaaccagtc gaagccgcag gtcttcgcgg cggtcgcgat gtccacggtg
     1021 cgggactgcc gcacgcccat gccgagcgcg agctcgccgg ccgcgagccg gcgcctggtg
     1081 tggttgggga cgatgtccat gtggggttct tcctgttgtc tcgggaggga ggcggcattg
     1141 taaccgctta cgcgggaggg gcgtgataag tgacaagtga taagtgataa gttactcgcg
     1201 ctcgcgcgga agtcggctcc tatcacctgt tacctatcac gcattttgtc tcagctccta
     1261 tcacctatca ctcatcacct atcacgcgtt tataatgcag gcatgcgctt cgtcctcctg
     1321 ctcatcgcgc tgaccgccgc agccgcgtcg gtcgcccagc cctatccgag caagccggtg
     1381 cgcttcctcg tcggctacgc gcccggcggc gcaaccgaca tcaacgcgcg catcgtggcg
     1441 ctcaagctca ccgagatgtg gggccagcaa gtcgtggtgg acaaccgtcc cggcgcgagc
     1501 ggaacgatcg ccgcggagct gacggcgaag gccgcgcccg acggctacac cctgctcgtg
     1561 gcgcctcaga ccagcgtggt ggccgcgccg ctcatcttcc gcaaggtcgg cttcgacacc
     1621 ctgaaggact tcgccgccct gtccatcatc ggctccacgc ccctgctgct ggtggtccac
     1681 ccctcgctgc cgccgaaaac cttcaaggaa ttcacctcct tcatgaagtc gaacgcctcc
     1741 aggctgagtt atgcgacggg cggcatcggc tctacgccgc atttcgccgc cgagctgctc
     1801 agcatctctc tcggcgtgaa aatggtgcac gtgccgtaca aaggggaaaa ccccgcggtg
     1861 gccgacatca tcagcggcca gataccgctg atgttctcca acatctccgc ggtgctgccg
     1921 ttcgtcaaag ccgggcgggt gcgcgcgctc gcgatcacca gcccgaagcg ctcgccgctc
     1981 gcccccgggt atccgaccgt cgcggaatcc gggttcccgg atttcgacgt cgcgacctgg
     2041 gcgggtctct acgcgcccgc agccatcaac cgcgagctgg gggcgcgcat cagcggcgac
     2101 atcgcgaagg tcatccggat gccggacgtg aaggaacgca tggccaacca gggcatcgac
     2161 cccgtcggca gcacgccgga cgagcatctc gcctacgtca aatccgagtt cacccgcatc
     2221 ggccgcgtcg cccgcgaagc caacatccgc gccgactagc ggcgcgggaa atgcgtgata
     2281 ggtgataggt gataggtgat aggtagttag gaacccgcgc gctacgcgga gttcttatca
     2341 cttatcacct gtcacgccgt tataattcgg gcatggccgc gatccgcgtc ttgcccgaat
     2401 tactcgtaag ccagatcgcc gccggcgaag tcgtcgaacg gcccgctgcc gcgctcaagg
     2461 agctgctgga gaacagcctc gacgcgggcg cgcgcgagat cgccgtccag ctctccggcg
     2521 gcggcgcgaa gctcgtcaag gtcaccgacg acggggacgg catcgggcgc gatgaattgc
     2581 ggctcgcgct cgcccgccac gccaccagca aaatcgcctc gctcgaggac ctggagcgcg
     2641 tgacgagcct cggcttccgc ggcgaggcgc tcgcgagcat cgccgccgtc tcgcgcctga
     2701 cgctcaccag ccgccgcgcc ggcgacaagc acgcctggac cgtgagcgcc agcggcgccg
     2761 aggtcacccg ggcggcgccc gcggcgctgg cggccggcac cagcgtcgaa gcgcgcgatc
     2821 tctacttcaa cacgccggcg cgccgcaagt tccttaagtc tgacgcgacc gagtacgcgc
     2881 actgcgagga agccttcaag cgggcagcgc tctcgcggcc cgacgccggt ttcacgctgc
     2941 agcacaacgg gcgggcgcaa tggcacctca agccgcagcc gctgcgcgag cgcatcggcg
     3001 cgctgctagg cgaagacttc aacgccaccg ccctcgcgct cgacgaggcg agcgccgggc
     3061 tgcgactcac cggggcgatc ggcttgccag cgcacgcgcg cgcgacccgc gaggcccagt
     3121 acttcttcgt caacggccgg ttcgtgcgcg acaagctcgc cgcccacgcc atccgccagg
     3181 cctatcagga cgtgctgcac cacgaccgcc acccggcgtt cgtgctgttc atcgaaatcg
     3241 accccggccg ggtggacgtc aacgtgcacc cgaccaagac cgaggtgcgc ttccgcgacc
     3301 cacgcggcgt gcaccagttc atctttcacg cgctgaacaa ggcccttgcc ggcgcggtcg
     3361 ccggggccgg gcaaacccgc ggcaccgccg tggctccggt ggcgggaatc gccgcagcgc
     3421 cgatgcggca ggccgccatg gcgttgaacg tcgccgaacc ggttgcgttc tacgaaaccc
     3481 tgttccggaa gcccggaccc gaggaagccg cgccggaaac ggaagacgcg ccgctgggat
     3541 tcgccctagc gcaactttcc ggcatctacg tgctcgccca gaaccggcac ggcctggtga
     3601 tcgtggacat gcacgccgcc catgagcgca tcatgtacga gaagctcaag agagcgctcg
     3661 aggagcgcgg tattccgatg cagccgctgc tggtgccggt gaccttcgcc acggaggcgc
     3721 tggatgtcgc gaccgcggac gagcatgggg acgtcctgcg cacgatcggc ttcgatatcg
     3781 ccgccgcctc gccgacctcg ctcatcgtgc gcgccgtgcc ggcgccgctc gccgacgcag
     3841 acgcgcgccg gctggctgtc gacgtgctgc gcgagatccg cgaaaccggc gcaagccggg
     3901 tgctcaccga gcggcgcaac gagctgctcg cgaccatggc gtgccacgcc gcggtgcgcg
     3961 ccaaccgcaa gctcacggtg ccggagatga acgctttgtt gcgcgaaatg gaggagaccg
     4021 agcgctccgg gcaatgcaac cacggccggc cgacgtggca ccagctgtcc ctcgcagaac
     4081 ttgaccgctt gttcatgcga gggaggtgat gcgtgatggg tgatgagtga tgggtcgttc
     4141 gaaatccaaa tccaagccgc gaaaacctgc taggaagcgt ttaaagacga agcgcgtgaa
     4201 ggctgccgcg aagcgtgctc ctgtcagcca cccatcaccc atcactcatc acccatcacc
     4261 tgtcgtcgag cagccgccac tcattactca tcactcatca ctcatcactg atttgcgggc
     4321 tccatcaggc cgcccgcaaa agtggccgcc ggccattttg ctgatgggcc ccaccgccag
     4381 cggcaagact gtggtggccg cgcggctcgc gcgggagctg ccgtgcgaga tcatcagcgt
     4441 ggactcggcc cagatctata agggcatgga catcggcacc gccaagccag aggccgagtt
     4501 gctcaagcgg gcgccgcatc acctgctcga cgtgatcgag ccgcacgaaa gctattccgc
     4561 ggcgcgcttc cgcgacgacg cgctggcgct gatgcgcgag atcaccgagc gcgaccgcat
     4621 cccgctgctg gtgggcggca ccatgctcta cttccgggcg ctgaccgaag ggctcaacga
     4681 gc
//
LOCUS       DBNGWZ010000181         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_18891, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000181 DBNGWZ010000000
VERSION     DBNGWZ010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_18891"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..333
                     /locus_tag="AC16CX_05405"
     CDS             118..333
                     /locus_tag="AC16CX_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3011983.1"
                     /translation="MTRADNALADLDCQGCQPSIDGLALGDTTPGLLVMVVAFVGLFR
                     FGVGMIIPLTGACGAAVLAYTFARQVI"
     gene            complement(345..1781)
                     /locus_tag="AC16CX_05410"
     CDS             complement(345..1781)
                     /locus_tag="AC16CX_05410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3011984.1"
                     /translation="MALRAFLILLLLAAAGVLADGLPDLGDASQAGFTPLQERRLGEN
                     IMREIRSDRSFYDDAEATDYINNLGNRLASRSPDTRQDFDFFLVRDGQINAFALPGGF
                     IGVNTGLILAAQSESELAGVLAHEISHVTQRHIARMIAQQKQSSVFSLASLAAALLLS
                     RVSSQAAEAAVAFGQAGAIQSQLNFTRDNERDADRVGLQILDQAGFDPHAMASFFERL
                     QRATRLYESGAPSYLRTHPLTFERIADMQNRTQGMSYRQVPDSLGFQLIRAKLRSQLD
                     TPREAVAFFEQSLAERKFLSEAASRYGLAASLMRVRDYARARKELAALRKLVQPNPIV
                     ETLACELASGAGAPEAALACYREALKAYPAYRALVYDYADALLRAGRPDEALRLVEAR
                     LQVVAGDYRLYQLQARSYAALNKRLSQYRAQAEAYVRLGNLTAAVEQLQIGLKSGDGD
                     FFQLSSAEARLKELRKLDEEMRREAGKR"
     gene            1852..2352
                     /gene="moaC"
                     /locus_tag="AC16CX_05415"
     CDS             1852..2352
                     /gene="moaC"
                     /locus_tag="AC16CX_05415"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR3011985.1"
                     /translation="MKRLTHIDARGRARMVDVADKGETRRTARAGGRIRVLPATLRQI
                     VSGTARKGDVLGVARTAAIQAAKRTAELIPLCHPLALTHISVDFKVDRKAGAVECLAT
                     VSTVGRTGVEMEALTAVAVGLLTIYDMCKAVDRGMHIERVQLLEKLGGKSGHWIARTA
                     KRRPSA"
     gene            complement(2428..>2869)
                     /locus_tag="AC16CX_05420"
     CDS             complement(2428..>2869)
                     /locus_tag="AC16CX_05420"
                     /inference="COORDINATES: protein motif:HMM:NF023015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HIR3011986.1"
                     /translation="PEQAEFWAMVYIGESVTNSFVARALRFAAEGASLCPLARQVMEL
                     HHRDEARHIAAARAFLEARAAAMGPLRRRAFAATFRFLLARFLTATLYPTPASLAALG
                     IPEPRRVAGAVRACPERRRLARACAAPALGFVAAAGLPVPRAAS"
BASE COUNT          479 a          975 c          973 g          442 t
ORIGIN      
        1 gttttttcgg atgggaccgc tttgtccatg tcatctcctt tgcgtacgca acgtcgcgca
       61 aaggcagcgc ttggacgcag ggcgtcttgg gaagcggggg gtctgcccga cccccgcgtg
      121 acacgagccg ataatgcgtt agccgatctc gactgtcaag gctgccagcc atcgatcgac
      181 gggctcgcgc tcggcgacac cacgccgggc ctgctcgtca tggtggtcgc gttcgtcggc
      241 ttgttccgct tcggcgtcgg catgattatc ccgctgaccg gcgcctgcgg tgcggcggtg
      301 ctcgcctata cgttcgcccg gcaggtcatc tagtgccggt cggctcaccg cttgccggct
      361 tcccgccgca tctcctcgtc cagcttgcgc agctccttca ggcgcgcctc ggcgctggaa
      421 agctggaaga aatcgccgtc gccgctcttc aggccgatct gcagctgttc gaccgccgcg
      481 gtcaagttgc ccagtcgcac ataggcctcc gcctgggcgc gatactggga aagacgcttg
      541 ttgagcgcgg cgtaactgcg cgcctgcagc tggtaaagcc ggtaatcgcc cgcaaccacc
      601 tgcaaccgcg cttcgaccag ccgcagcgcc tcatccggcc gtcccgcccg caacagcgca
      661 tcggcatagt cgtaaaccaa ggcacggtag gcgggataag ccttcagcgc ctcgcggtaa
      721 caggcgagcg ccgcctccgg agcgcctgcg ccggacgcca gctcgcaggc cagcgtttcc
      781 acgatcgggt ttggctgcac cagcttgcgc aaggccgcga gttccttgcg cgcccgggcg
      841 tagtccctca cgcgcatcag gctggctgca agtccgtagc ggctcgccgc ctcgctcagg
      901 aacttgcgct cggcgaggct ttgctcgaag aacgcgacag cctcgcgcgg ggtgtcgagc
      961 tgcgaccgga gcttagcgcg gatcagctga aagcccaggc tgtcgggaac ctggcggtag
     1021 ctcatcccct gcgtccggtt ctgcatgtcg gcgatgcgct cgaaggtcag cgggtgcgtg
     1081 cgcagatacg acggcgcgcc gctctcgtag aggcgggtcg cgcgctgcag ccgctcgaaa
     1141 aagctcgcca tcgcgtgcgg atcgaagccc gcctggtcga ggatctgcag tccgacgcgg
     1201 tcggcgtcgc gctcgttgtc gcgcgtgaaa ttgagctggc tctggatcgc gccggcctgc
     1261 ccgaaggcga cggcggcttc cgccgcctgg gagctcacgc gcgagagcag cagcgccgcc
     1321 gccaacgacg ccagcgaaaa gacgctgctc tgcttctgct gcgcgatcat gcgcgcgatg
     1381 tggcgctggg tgacgtgcga gatttcgtgc gccagcacgc ccgccagctc cgactcgctc
     1441 tgcgccgcga ggatcaatcc ggtattgacg ccgatgaatc cgcccggaag cgcgaaggcg
     1501 ttgatctggc cgtcgcggac gaggaagaaa tcgaagtcct ggcgcgtgtc cgggctgcgc
     1561 gaggcgaggc ggttgcccag attgttgatg tagtccgtcg cctccgcgtc gtcgtaaaaa
     1621 ctccggtcgg accggatctc gcgcatgatg ttttcgccca gccgccgctc ctgcagcggc
     1681 gtgaaacccg cctgcgacgc gtcgccgagg tcgggcaatc catcggccag cacgccggct
     1741 gcggcaagca gcaacaggat gaggaatgcg cgcaacgcca taatgctatg ataattcgga
     1801 tgtgcgggag ttcctacaaa aggcccgcgc ccgacaaatg aaaaccgcga cgtgaaacgg
     1861 ctcacccaca tcgacgctcg cggtcgggcg cgcatggtcg acgtcgcgga caaaggtgaa
     1921 acccggcgca ccgcgcgtgc cggtggccgc atccgcgtgc tgcctgcgac cctcaggcaa
     1981 atcgtctccg gaaccgcgag gaagggcgac gtcctcggcg tcgcgcgcac cgcggccatc
     2041 caggccgcga agcggaccgc ggagctgata ccgctgtgtc acccgctcgc gctcacccac
     2101 atcagcgtcg acttcaaggt ggaccgcaaa gcgggcgccg tggagtgcct ggcgaccgtt
     2161 tccaccgtcg ggcgcaccgg agtcgagatg gaagccctga cggcggttgc ggtgggcctg
     2221 ctcaccattt atgacatgtg caaagccgtg gaccgcggca tgcacatcga gcgcgtacag
     2281 ttgctcgaga aactgggcgg caagtcggga cactggattg cgcgcacggc gaagcgccga
     2341 ccctccgcct gaaccgatga tcccaccagc gcatcaaaga gctggtttgg aaaagaccgg
     2401 cgtttaggcc tgggccggcc gcggccgtca cgacgccgcc cgcggcacgg gaaggccggc
     2461 ggcagcgacg aaaccgagtg ccggggcggc gcaggcgcgg gcgagccggc gtcgctcggg
     2521 acaggcgcgg accgcgcccg cgacccgccg aggctcgggg ataccgagcg ccgcgaggct
     2581 cgccggcgtc ggataaagcg tcgcggtgag aaagcgcgcg agcaggaacc tgaacgtcgc
     2641 ggcgaaggcc cgacgacgca gcggtcccat ggcggcggca cgcgcctcga ggaacgcgcg
     2701 cgccgccgcg atatggcgcg cctcgtcgcg atgatgcagc tccatcacct ggcgggcgag
     2761 cggacaaaga gacgcgcctt ctgcggcgaa gcgcagcgcg cgcgccacga agctgttggt
     2821 caccgactcc ccgatataga ccatcgccca gaactccgcc tgttccggc
//
LOCUS       DBNGWZ010000182         1541 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2159842, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000182 DBNGWZ010000000
VERSION     DBNGWZ010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2159842"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16CX_05425"
     CDS             <1..353
                     /locus_tag="AC16CX_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3011987.1"
                     /translation="AQGANCSIATNNVLNPFTPYGDCSLVRIANLYANVVQRGTEEDL
                     TECFALLTDRPARILRKDDYGIAVGNLADLIVWNAKTPAEVIATVAQPLAGFKRGRRV
                     FTREMPTLHRPFKS"
     gene            complement(536..1066)
                     /locus_tag="AC16CX_05430"
     CDS             complement(536..1066)
                     /locus_tag="AC16CX_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR3011988.1"
                     /translation="MNKLILTALVSAGLAAAVPQAIAQTADAEGGGPQARPFAQRQHP
                     EQRAFRSPTDRVEAQLAYLKTALKITDAQEAQWNAFAEVRRKHAREATERMQQFRSRM
                     AERQQGARPTAVERMERRQAMLAVASTRLSETLAAAKPLYAALSPEQKQIADELLAPR
                     GRGGFHHRGGHGRGRA"
BASE COUNT          273 a          475 c          526 g          267 t
ORIGIN      
        1 tcgcgcaggg ggcgaactgc tccattgcga ccaacaacgt gctgaatccg ttcacgccct
       61 acggcgattg ctcgctggtg cgcatcgcga acctgtacgc gaacgtcgtg cagcgcggca
      121 cggaagaaga cctgaccgag tgcttcgcgt tgctgaccga tcgacccgcg cgcatcctgc
      181 gcaaggacga ttacggcatc gctgtcggca atctggccga cctcatcgta tggaatgcga
      241 aaacgccggc cgaggtcatc gcgaccgtgg cgcagcccct ggcgggcttc aagcgcggcc
      301 ggcgcgtatt cacgcgcgaa atgccgacgc tgcatcgtcc tttcaaatca tgaggcgtga
      361 cgagtgacgg gtgatgggtg acggggacgg ctccgccaga aggatgagaa ggcagaagga
      421 tgaaagcgga aggcgaaagg aggagggcgg aaggatgaag gatgaaaaag gccggcttgc
      481 gccggccctt gtctttgcgg ctgaaaacgg aggcggtgcc gcgtccgctt gcgagttacg
      541 cccttccgcg accgtgaccg ccgcggtgat ggaacccgcc gcgcccgcgg ggcgcgagca
      601 gctcgtcggc gatctgtttc tgctcgggcg agagcgcggc gtagagcggc ttcgcggccg
      661 ccagggtttc actcagccgc gtggaggcta ccgcgagcat ggcctgcctg cgctccatgc
      721 gctcgaccgc cgtgggccgg gcgccttgct gccgttcggc catccgcgac cggaactgct
      781 gcatgcgctc ggttgcctcg cgcgcgtgct tgcgccgcac ctcggcgaat gcgttccact
      841 gcgcttcctg cgcgtcggtg atcttcaagg cggttttcag gtaggcgagc tgcgcctcga
      901 cccggtcggt cggcgaccgg aacgcgcgct gctcagggtg ctgccgttgc gcgaaagggc
      961 gagcttgcgg cccgccgcct tccgcgtccg ccgtctgggc gatcgcctgc ggaacagccg
     1021 ccgccaggcc ggcggaaaca agggccgtca gaatcaactt gttcatggtt tccctccaga
     1081 gttgttgagg aatcatccgc tccccgcggg ttgcgggaag ttcatcagct cagaccggga
     1141 cgaggtgagt cccgttcggc ggaatgttgt aacggtttgt aagtgggaga gcaaaaaggg
     1201 gaaaggtgaa aggcaggcag aaggcaagca agttcactca ccactcacgc cgtttctcgc
     1261 acggcggagg cgcgacttgt tacactatag gctggcaact ttgaaaggag gtgatcccgt
     1321 gagagacctt ctaggcaaca ccgaccggca cggctcgaac cgcggatcaa cccgcgacga
     1381 aaaggagttc gaccggcgct aggccggcgt gcccgagcaa caccgacgcc ccgcggaccc
     1441 cgggtctgcg gggcgttttt ctttcactag gcactaatcc gggaacggac tacggtttcg
     1501 ctttcggcgc ttcttgtgga ttcgcgagct cggcttgccc c
//
LOCUS       DBNGWZ010000183         1660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_773425, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000183 DBNGWZ010000000
VERSION     DBNGWZ010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_773425"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="sufC"
                     /locus_tag="AC16CX_05435"
     CDS             complement(<1..518)
                     /gene="sufC"
                     /locus_tag="AC16CX_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR3011989.1"
                     /translation="MLEVKGLRAKVAGVEILKGIDLTVRAGEVHAVMGPNGSGKSTFA
                     KALVGHPAYEVTGGTVAYEGRNLFEMAPEERARAGLFLGFQYPVEIPGVSNSAFLRLA
                     YNTLQAARGKDELDPLEFDDHARETMRLLDMDPGFLDRSVNEGFSGGEKKRNEILQMA
                     LLEPRLAILDET"
     gene            complement(539..>1660)
                     /gene="sufB"
                     /locus_tag="AC16CX_05440"
     CDS             complement(539..>1660)
                     /gene="sufB"
                     /locus_tag="AC16CX_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR3011990.1"
                     /translation="FEKLGVPMNERAKLAGVAVDVIFDSVSVATTYKQKLAEVGIIFC
                     SISEAVREHPELVRKYLGTVVPTGDNYYAALNSAVFTDGSFCFIPRGVKCPMELSTYF
                     RINTESSGQFERTLIVAEEGAHVSYLEGCTAPKFDTNQLHAAVVELVALDDAEIKYST
                     VQNWYAGDENGVGGIYNFVTKRGLCKGMNSKISWTQVETGSAITWKYPSCVLLGDNSI
                     GEFYSVALTNHHQQADTGTKMVHIGRNTRSRIISKGISAGQSNNSYRGLVKIGPRAEN
                     ARNYSQCDSMLIGSKCGANTFPYIQVQNNGATVEHEATTSKIGEEQLFYFASRGIEAE
                     EAVSMIINGFCRDVFQQLPMEFAVEATKLLGLKLEGSVG"
BASE COUNT          269 a          529 c          535 g          327 t
ORIGIN      
        1 tcggtctcgt cgaggatcgc gagccgcggc tcgagcagcg ccatttgcag gatctcgttc
       61 ctcttcttct cgccccccga gaacccctcg ttcaccgagc ggtcgaggaa acccgggtcc
      121 atgtcgagca gcctcatcgt ttcgcgggca tggtcgtcga attcgagcgg gtcgagctcg
      181 tccttgccgc gcgccgcctg cagcgtgttg taggcgagcc gcaggaacgc gctgttcgag
      241 acgccgggga tctcgaccgg gtactgaaag ccgaggaaga ggccggcgcg ggcgcgctcc
      301 tccggcgcca tctcgaagag gttgcggcct tcgtaggcga ccgtgccgcc ggtgacttcg
      361 tacgcgggat ggcccacgag cgccttggcg aaggtgctct tgccggagcc gttggggccc
      421 atgacggcgt gcacctcgcc ggcgcgcacg gtaaggtcta tgcccttcag gatctcgacc
      481 ccggccacct tggcgcggag gcctttcacc tcgagcagca ctctggcgtc cgtgcggatc
      541 atcccacgct cccttcgagc ttgaggccca gcagtttcgt cgcttccacc gcgaattcca
      601 tcggcagctg ctggaacaca tcgcggcaga agccgttgat gatcatcgag accgcctctt
      661 cggcctcgat gccgcggctc gcgaaataga acagctgttc ctcgccgatc ttggaggtgg
      721 tcgcttcgtg ctcgaccgtc gcgccgttgt tctgcacctg gatgtaggga aaggtgttgg
      781 cgccgcactt cgagccgatc agcatggaat cgcactggga gtagttgcgc gcgttctcgg
      841 cgcgcgggcc gatcttcacg agaccgcggt agctgttgtt ggattgcccc gcggagatgc
      901 ccttgctgat gatgcggctc ctcgtgttcc tgccgatatg caccatcttg gttccggtat
      961 cagcctgctg gtggtgattg gtgagcgcga ccgagtagaa ctcgccgatc gaattgtcgc
     1021 cgagcagcac gcaggaaggg tacttccagg tgatcgcgga accagtctcg acctgggtcc
     1081 aggagatctt ggaattcatg cccttgcaca gcccgcgctt ggtgacgaag ttgtagatgc
     1141 cgcccacgcc gttctcgtca ccggcgtacc agttctgcac ggtcgagtac ttgatctcgg
     1201 cgtcgtcgag cgcgaccagc tccaccaccg cggcgtgcag ctggttggtg tcgaacttgg
     1261 gtgccgtaca gccctcgagg taggaaacgt gggcgccctc ctcggcgacg atcagcgtcc
     1321 gctcgaactg cccggaggat tcggtgttga tgcggaaata ggtcgagagc tccatcgggc
     1381 acttcacgcc ccgcgggatg aaacagaacg agccatcggt gaacaccgcg gaattgagcg
     1441 cggcgtagta gttgtcgccg gtcggcacga ccgtgcccag gtacttgcgc accagctcgg
     1501 gatgctcgcg caccgcttcc gagatcgagc agaagatgat gccgacctcg gcgagcttct
     1561 gcttgtaggt ggtcgcgacc gacaccgagt cgaagatcac gtccaccgcc actccggcga
     1621 gcttggcccg ctcgttcatc ggcacgccga gcttctcgaa
//
LOCUS       DBNGWZ010000184         3638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_523445, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000184 DBNGWZ010000000
VERSION     DBNGWZ010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_523445"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="AC16CX_05445"
     CDS             <1..681
                     /locus_tag="AC16CX_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HIR3011991.1"
                     /translation="ASYTGGAIAPGELISIFGSNFGDSGVLVYAPENNRVPFVLGPTK
                     VWIGGQFGAITAVTPNQINVFVPQWLEPGKSVDVVVQVDDVASAPVSVPVAKAAPGLA
                     TADGSGSGQEAILNQDGSRNSMANPAERGSIVALFGTGEGLVWPRYYCCYLTISTPYP
                     VPIEPVAVTIGGQQAEVLYAGSAPFLPAGVFQINARLPVGIAPGNAPVVVTIGGVATT
                     KQVTVAAR"
     gene            1363..2382
                     /locus_tag="AC16CX_05450"
     CDS             1363..2382
                     /locus_tag="AC16CX_05450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR3011992.1"
                     /translation="MAKRALITGITGQDGSYLAELLVSKGYEVHGIVRRVALEDPDHR
                     LCRLSAVRDRITLHVASIESYASVFQVVASVAPDECYHLAAESFVSYSFDDEFSTFNV
                     NINGTHFLLAALRHLAPQCRFYFAGSSEMFGKVEQVPQNENTRFHPRSSYGISKVCGY
                     ELTRNYREAYHAHATSGILFNHESPRRAFEFVTRKITSGIARIAGGEAKELRLGNLEA
                     KRDWGHAREYVEAMWLMLQQPQPDDYVVASGETHSVREFCELAFAEVNLDYRDFVKVD
                     ERFYRPAEMDLLIGDATKAKNVLGWAPRTPFRDLVREMVREDIKSYQPCVPGHRLRAL
                     KFISR"
     gene            2987..>3638
                     /locus_tag="AC16CX_05455"
     CDS             2987..>3638
                     /locus_tag="AC16CX_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR3011993.1"
                     /translation="MGCEQGQDEEKPVHRVWVDAFEMAIFQVRHSDYGVFLEATGHPA
                     PPHWNDPDFHHPDQPVVAVSWLEGVKYCEWLTGVSGRRYRLPTEAEWERAARGGREGR
                     LYPWGNEPPQTRPEYLRRWGGEVRGPLPVGQDAPNPYGLCDIGENVHEWCADWFYKHY
                     YAQSPQRNPRGPEAGDRRASRGGSWRHHIKVSRCAARSSIPPEFQYADYGFRVVREI"
BASE COUNT          668 a         1139 c         1154 g          677 t
ORIGIN      
        1 gccagctaca cgggcggggc gattgcgccg ggggaactga tctccatctt cggttcgaac
       61 ttcggcgact cgggcgtgtt ggtctacgcg cccgagaata accgcgtgcc gtttgtactc
      121 ggcccgacga aggtgtggat cggcgggcaa ttcggcgcga tcacggcggt cacgcccaac
      181 cagatcaacg tcttcgtccc gcaatggctg gagccaggca aatctgtcga tgtagtcgtc
      241 caagtggatg acgtggcgtc cgcgccggtg agcgtgccgg tggcgaaggc cgcgcccggg
      301 ctcgcgaccg ccgacgggag cgggtcgggg caggaggcca ttctgaacca ggacggatcg
      361 cggaactcga tggcgaaccc ggcggagcgc ggctccatcg tggcgctgtt cgggaccggc
      421 gagggcctgg tttggccaag gtactactgc tgctacctca ctatctcgac cccctacccg
      481 gtcccgatag agcccgttgc cgtcaccatc ggcgggcagc aggccgaggt cctctacgcc
      541 gggtccgcgc cgttcctgcc ggcgggcgtg ttccagatca acgcgcggct gccggtcggg
      601 atcgcgccgg ggaacgcgcc ggtcgtggtg acgatcggcg gcgtggcgac gacgaagcag
      661 gtcacggtcg cggcgcgata actgcgcacg cgtcagataa tcacgatcat ggctcatcgg
      721 ttgttgttgc tctttgccgc gaccgtctgc tcggccgcgg atctgccgct tacactcgct
      781 tacactggat gaccgactgg gcgacccctt ctggcagggg gttgcccgag ctgggcaaca
      841 ccgatccgcc gttcgagatc ggaaaggtgc tgcgcgtccc gccgatgccg gcccaatggg
      901 accacccggc cttggcgcga cgtccctgaa ttcgagcttc cccgcaacaa gccgtttccc
      961 cgccccgcgc gctacgctac gaaagtcaag tgggttcacg tcggccgcac cctggccctg
     1021 ctgttccgca tgaacgagcc cgagccagtg tcgcccgcgc cggcggacgc gacagcgggg
     1081 tcaccggcga cgaccatgtg gcgttatacc tggccacgaa cggttcggct ttacgcgaga
     1141 tcgccgtcag cagtgtcggg gcgatccgcg actcgcgcgg cggcggcctg cggatgagat
     1201 cgcagaccaa ttggaacgcg cgcatcggca cggctcaacg agtcgggcgt cacatcgaac
     1261 gggtccgatc aacctatcag aggtggttcg gccggcggct gtgattgctc attacccgac
     1321 accaatgaac ctacgtagat ccggtgctag gatagggtgt ttatggccaa acgcgccctc
     1381 attaccggga tcacggggca agacgggtcc tacctcgcgg aactgctggt atcgaaaggg
     1441 tacgaggttc acggcatcgt ccgccgcgtg gcgctggagg atccggatca ccggctctgc
     1501 cgcctcagcg ctgtccggga ccgaatcacg ctgcacgtgg cttccatcga gagctatgca
     1561 agcgtgttcc aggtggtagc cagcgtggcg cccgatgagt gttaccattt ggctgccgaa
     1621 agcttcgtca gctactcgtt cgacgacgag ttctcaacgt tcaacgtcaa tattaacggg
     1681 acgcacttct tgcttgccgc attgcgtcat ctcgcgccgc aatgtcggtt ctattttgcg
     1741 ggatcgagcg agatgttcgg caaggtcgag caggtgccgc agaatgagaa tacccgtttc
     1801 catccgcgct ccagctacgg catcagcaag gtttgcggct acgaactgac gcgaaactac
     1861 cgcgaagcgt accatgctca cgcgaccagc ggcatcctct tcaatcacga gtcaccgcgc
     1921 cgcgcattcg agttcgtcac ccggaagatc acgtcgggca tcgcccgcat cgccggcggc
     1981 gaggcgaagg agctgcggct tggaaacctg gaagccaaac gagactgggg ccatgctcgc
     2041 gagtatgtag aagccatgtg gctgatgctt cagcagccgc agccggacga ctacgtagtg
     2101 gcctcgggcg agacgcactc ggtgcgggag ttctgcgagc tggcattcgc tgaggtgaac
     2161 cttgattacc gggactttgt taaggtagac gagcgcttct accgtcccgc tgagatggac
     2221 ctgctgatcg gcgacgccac caaggccaag aacgtgctcg gctgggcgcc gaggacccct
     2281 ttccgcgacc tggtgcgcga gatggtgcga gaagacatca agagctacca gccgtgcgta
     2341 ccgggtcacc gcttgcgggc cctgaaattc atttcgcgtt agccgacgta ctggcgatgg
     2401 aggacttcga aacggttttc aaccagacgc tctctcagac cagggcgcga aaccaccgtc
     2461 ccacccggct taagaacacg cgttcgcaag caactgggcg cccggttgca actgagatcc
     2521 gattcctcgc tggccttctc gcaatcgtga tcaaccctca gcttctcaag ccctggtacc
     2581 ccagattgat tcgtaaataa catagcaatg agggctgatc gatcacgacg gcgctatcga
     2641 ctccgtttct agcgtctcta acttgtactt ccacttgaat accaccggct cttcgttcag
     2701 ttccttaaag tagagctccg cgcgccttca actccgcctt cgagccgacg cggatccctc
     2761 gcagcagcgt cttggccagc ttgccgaaga acgattcgat aaggttgagc cacgatccgt
     2821 gcttgggggt gaagaggaac tcgaaacgat tcggaacgtc cgccaggtaa cctcgcgtct
     2881 ccttacgaag gacccgagcc agaacgtccg agttgcggct agaatggttg cggtgccgga
     2941 gtttcagcaa ccagcctgtg tgctcatccc ttccggatac ttcttgatgg gctgcgagca
     3001 ggggcaagac gaagaaaagc ccgtgcatcg cgtctgggta gatgccttcg agatggcgat
     3061 ttttcaggtg cggcatagcg attacggagt ttttctcgag gctacgggcc accccgcacc
     3121 gccgcattgg aatgacccgg acttccatca tcccgatcag cccgtagttg ctgtgagctg
     3181 gttggaaggg gtcaaatatt gcgagtggct cacgggtgtg agcggccgtc gctatcgcct
     3241 tcccaccgag gcggaatggg agcgggcggc gcggggaggt cgcgaggggc gcctctatcc
     3301 ctggggtaac gagccgccgc agaccaggcc cgagtacttg cgccggtggg gcggcgaagt
     3361 ccggggccct cttccggtcg gccaggacgc gccgaacccc tatgggctgt gcgatatcgg
     3421 cgagaacgtg cacgagtggt gcgccgactg gttttacaag cactactacg cgcaatcacc
     3481 ccaacgcaat cctcgcgggc ccgaggcagg cgaccggcgc gcctcccgcg gcggttcctg
     3541 gaggcaccac atcaaggtga gccgctgcgc cgctcgctcc agcatccccc cagaattcca
     3601 gtacgccgac tacggctttc gcgtggtgcg ggaaatct
//
LOCUS       DBNGWZ010000185         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1781869, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000185 DBNGWZ010000000
VERSION     DBNGWZ010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1781869"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..454)
                     /locus_tag="AC16CX_05460"
     CDS             complement(77..454)
                     /locus_tag="AC16CX_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3011994.1"
                     /translation="MKYLCLVYLDEKRLHELPDEECVAYDTTIRKSGHCIASEALESV
                     QTATTVRVRNGKTSVTDGPFAETKEQLAGFYMIEARDLNEAIQLASKIPPARVGSIEV
                     RPIRPIRETVAAARGNETADKRR"
     gene            complement(466..957)
                     /locus_tag="AC16CX_05465"
     CDS             complement(466..957)
                     /locus_tag="AC16CX_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3011995.1"
                     /translation="MQRIIPFLWFDGKAEEAVNFYVSLFRNSGIDTVTRYGKEGAQAS
                     GMPPDTVMTIAFRIEGQDFVALNGGPYFKFTEAISFVVNCESQDEVDYYWERLSTGGD
                     PAAQRCGWLKDRYGVSWQIVPKVLGSLLKGPDPARSGRVMQALLQMTKLDIARLKQAH
                     EQN"
     gene            complement(962..1348)
                     /locus_tag="AC16CX_05470"
     CDS             complement(962..1348)
                     /locus_tag="AC16CX_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3011996.1"
                     /translation="MKYLCLICAEKVMEQMSEADAEKHYQDYEAFLASIRKSGHYVGC
                     NRLLPPAEATTIRVRNGKVAATDGPWIETKEQLGGYFVIEARDLNEAIRVAARIPGAS
                     IGCVEVRPIAEDERTLRMLSEGHGKA"
     gene            complement(1345..>1674)
                     /locus_tag="AC16CX_05475"
     CDS             complement(1345..>1674)
                     /locus_tag="AC16CX_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3011997.1"
                     /translation="LMHAMIRIEGSAVMLVDEMPEWGAFGPKSLKGSPVTIHLYVEDV
                     DSFVARAVGAGAKITMPVADMFWGDRYGKLEDPFGHHWSVATHIRDVTPEEMQQAMRK
                     MGGQEAR"
BASE COUNT          265 a          562 c          493 g          354 t
ORIGIN      
        1 tcgggcggga gtttgcttgc gaaccgctgt cttccgcgcc ttgcgtgcca tcgttctctc
       61 cttttcaaat ctgcgcttat ctgcgtttat ctgcggtttc atttccgcgt gctgccgcga
      121 ctgtttcgcg gatcggccgg atcggccgca cctcgatgct gcccacgcgc gccggcggaa
      181 tcttcgaggc gagctggatg gcctcgttga ggtcccgggc ctcaatcatg tagaagccgg
      241 cgagctgctc cttggtctcg gcgaacggcc cgtcggtgac ggacgttttg ccgttcctca
      301 cccgcacggt ggtggcagtc tgcacggact cgagggcttc cgaggcgatg cagtggccgc
      361 tcttccgtat cgtggtgtcg taggccacgc actcctcgtc gggcagctcg tgcagccttt
      421 tctcgtcgag gtacaccagg cacaggtatt tcatcgccat ccacctcagt tctgttcgtg
      481 cgcctgcttc aggcgcgcga tgtcgagctt ggtcatttgc agcagggcct gcatcacgcg
      541 acccgacctg gcgggatcgg gacccttcag cagcgagccc agcactttcg gaacaatctg
      601 ccaggacacg ccgtagcggt ccttcagcca gccgcagcgc tgcgcagcgg gatcaccgcc
      661 ggtagacagt cgttcccagt aatagtccac ttcgtcctgc gactcgcaat tcaccacgaa
      721 ggaaatggcc tctgtgaact tgaaataggg cccgccgttc agcgccacaa aatcctgccc
      781 ctcgatgcgg aacgctatgg tcatgaccgt gtccggcggc atgccggaag cctgcgcccc
      841 ttccttgcca tagcgggtaa ccgtgtcgat gccggaattc ctgaacagcg aaacgtagaa
      901 atttaccgcc tcttcagcct tgccgtcgaa ccacaggaac gggatgattc tctgcacggg
      961 tttacgcctt gccgtggcct tcgctgagca tgcgcagcgt ccgctcgtcc tcggcgatcg
     1021 gccgcacctc gacgcagccg atggaggcgc ccggtatccg ggcggccacc cgtatcgcct
     1081 cgttgagatc gcgcgcctcg atcacgaagt agccgccgag ctgctccttg gtctcgatcc
     1141 aggggccgtc ggtcgccgcg accttgccgt tgcgcacgcg gatggtggtt gcttcggcgg
     1201 gcggtagcag ccgattgcag ccgacgtagt ggccgctctt gcgtatcgac gcgaggaacg
     1261 cctcgtaatc ctggtagtgc ttttccgcat cggcctcgct catttgctcc atcacctttt
     1321 cggcgcagat caggcacagg tatttcatcg cgcctcctgg ccgcccattt tccgcatcgc
     1381 ctgttgcatc tcctccgggg tcacgtcgcg aatgtgcgtg gccaccgacc aatgatggcc
     1441 gaacgggtct tcgagcttgc cgtaacggtc gccccaaaac atgtccgcca ccggcatcgt
     1501 gatcttcgcc cccgcgccga cggcgcgcgc gacgaacgag tcgacatcct cgacatagag
     1561 gtggatcgtg accggcgagc ccttcagaga cttcggcccg aacgcgcccc actcgggcat
     1621 ttcatcgacc agcatcacgg ccgagccttc gatacggatc atcgcgtgca tgag
//
LOCUS       DBNGWZ010000186         3169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_648580, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000186 DBNGWZ010000000
VERSION     DBNGWZ010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_648580"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC16CX_05480"
     CDS             <1..554
                     /locus_tag="AC16CX_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3011998.1"
                     /translation="GAAGLIAGAFSMAAGEYVSVRSQREMYEYQIGLEREELEHYPEA
                     EAEELALIYEARGHPREEAVSMAKNLIADPERALATLAREELGLNPEDLGSPWGAALF
                     SFFSFAAGAALPLAPFLFAAGAASLTIAVTLTAVALFAVGTTLSLFTGRSAVTGGLRM
                     LAIGAAAGGFTYLIGKLLGVTLA"
     gene            complement(616..1002)
                     /locus_tag="AC16CX_05485"
     CDS             complement(616..1002)
                     /locus_tag="AC16CX_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3011999.1"
                     /translation="MKHFEPKEAYEFLKKNPNTIFIDCRSEMEYLFVGHPTGAILVPW
                     NDGPNWEVNPDFVAHVKKAASVDRPIVLICRSGNRSLDAGRALEEAGFTKVWNVLHGF
                     EGELDESHHRNAKTGWRHEGLPWEQC"
     gene            1131..>3169
                     /gene="metH"
                     /locus_tag="AC16CX_05490"
     CDS             1131..>3169
                     /gene="metH"
                     /locus_tag="AC16CX_05490"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3012000.1"
                     /translation="MPDRTAQLDALLKQRILMLDGAMGTMIQSYRLSEADYRGQRFAD
                     HPHDLKGNNDLLVLTRPQVIREIHEQYLAAGADVIETNTFNSTSISQADYHLQHVVHE
                     LNLEAARLARAMADDYTARNPGRPRFVAGALGPTNRTASISPDVNDPGFRNVSFDELA
                     AAYGEAVHGLVEGGVDILLIETIFDTLNAKAAIFAIDEYFAKRNIHLPVILSGTITDA
                     SGRTLSGQTPEAFWNSVRHARPLAVGLNCALGAKLMRPYIEEISKIADVYVSAYPNAG
                     LPNPLAPTGYDETPEQTAGYLLDFAQSGFINIAGGCCGTTAAHIRAIAEALKGVPPRR
                     LPAIERKTRLAGLEALNIGDDALFVNVGERTNVTGSRAFSRLVLAGDYQEALSIARQQ
                     VENGAQMIDVNMDEAMLDSKQAMTTFLNLIASEPDISRVPLMIDSSKWEVIEAGLKCM
                     QGKPVVNSISLKEGKDEFVKHAKLARRYGAAVIVMAFDQKGQADTLERRVEIAKRCYH
                     ILVDELGFPPEDVIFDPNIFAIATGIEEHANYGKDYIEAARVIRQTLPYAKVSGGVSN
                     ISFSFRGNDPVREAIHTAFLYHAVKVGMTMGIVNAGQLGIYEEIPKELLEHVEDILFN
                     RRPDAAERMVAFAETVKGGKRRDTEDLEWRKAAVEERLKHALVQGIANWIVEDTE"
BASE COUNT          560 a         1051 c         1028 g          530 t
ORIGIN      
        1 cgggcgccgc aggcctcatc gcgggggcgt tctcgatggc ggccggcgag tacgtctcgg
       61 tgcgctcgca gcgcgagatg tacgagtacc agatcgggct cgagcgcgag gagctcgaac
      121 actaccccga ggcggaggcg gaggaactcg cgctgatcta cgaggcgagg gggcacccac
      181 gcgaagaggc ggtttcgatg gcgaagaacc tgatcgccga tccggaacgg gcgctggcga
      241 cgcttgcgcg cgaggagctg ggcttgaacc ccgaggacct cggctcgccg tggggcgcgg
      301 cgctgttctc attcttttcc ttcgccgccg gggcggccct cccgctcgcg ccgtttctct
      361 tcgccgcggg agccgcaagc ctgacgatcg ctgttacgct gaccgccgtc gccctattcg
      421 cggtcggcac gacgctctcg ctcttcaccg gccgcagcgc cgtcaccggc gggctgcgca
      481 tgctcgcgat cggcgccgct gcgggcggct tcacctacct catcggcaag ctgctcggcg
      541 tgacgttggc gtagctgtca gccgtcagcc atcagctttc agcaagaccc taagctgacc
      601 gctgaacgct gcttgtcagc actgctccca cggcagcccc tcgtggcgcc acccggtctt
      661 cgcgttgcgg tggtggctct cgtcgagctc gccttcgaag ccgtgcagca cgttccagac
      721 cttggtgaag ccggcttcct cgagcgcgcg gccggcgtcg agcgagcggt tgccgctgcg
      781 gcagatcagc acgatcggcc ggtcgacgct cgccgctttc ttcacgtgcg cgacgaaatc
      841 gggattcacc tcccaattcg gcccgtcgtt ccacggcacg aggatcgcgc ccgtggggtg
      901 acccacgaac aggtactcca tctcgctgcg gcaatcgatg aagatcgtgt tcgggttctt
      961 cttcaggaac tcgtaggctt ctttcggctc gaagtgcttc attgcgaaat ttcgcttgct
     1021 caaacgggag cgaaattata gtcgcaaccg cccgcaaacc gccagccggt gcggctttgg
     1081 cggtagaatt accacttttt gcgtgagggt tttccgtctc acgcgcgcgc atgccggacc
     1141 gaaccgcaca actcgacgcc ctgctcaagc agcgcatcct gatgctcgac ggcgcgatgg
     1201 gcaccatgat ccagagctac cggctctcgg aggcggatta ccgcgggcag cggttcgcgg
     1261 atcatccgca cgacctcaag ggcaacaacg acctgctcgt gctcacgcgc ccgcaggtga
     1321 tccgcgagat ccacgagcag tacctcgcgg cgggcgccga cgtgatcgag accaacacct
     1381 tcaactcgac ctcgatctcg caggccgatt accacctgca gcacgtggtg cacgagctga
     1441 atctcgaagc ggcgcggctc gcgcgcgcga tggcggacga ctacaccgcg aggaatccgg
     1501 gccggccgcg cttcgtcgcg ggcgcgctcg gtcccaccaa ccgcaccgcc tcgatctcgc
     1561 ccgacgtcaa cgaccccggc ttccgcaacg tgagtttcga tgagctcgca gcggcgtatg
     1621 gcgaagcggt gcatggactg gtcgaagggg gcgtggacat tctgctcatc gaaactatct
     1681 tcgacacatt gaatgccaag gccgcgatct tcgcgattga tgaatatttc gcgaaacgaa
     1741 atattcatct gcccgtgatc ctttccggca ccatcacgga cgcgtcgggc cgcacgctgt
     1801 cgggccagac cccggaggcg ttctggaact cggtgcgcca cgcgcgcccg ctcgcggtgg
     1861 gactgaactg cgcgctcggc gcgaagctga tgcgccctta catcgaggag atttccaaga
     1921 ttgccgacgt ctacgtctcg gcctatccca atgccgggct gccgaacccg cttgcgccga
     1981 ccggctatga cgagacgccg gagcagaccg ccggctacct gctcgacttc gcgcagagcg
     2041 ggttcatcaa catcgccggc ggatgctgcg gcaccactgc ggcgcacata cgggcgatcg
     2101 ccgaggcgct gaagggcgtc ccgccgcggc ggctgccggc catcgagcgc aagacccggc
     2161 tcgcgggcct cgaggcgctc aacatcggcg acgatgccct gttcgtcaac gtcggcgagc
     2221 gcaccaacgt caccggctcg cgcgcgttct cccggcttgt cctcgcgggc gactatcagg
     2281 aggcgctgtc gatcgcgcgc cagcaggtgg agaacggcgc gcagatgatc gacgtcaaca
     2341 tggacgaggc gatgctcgac tcgaagcagg cgatgacgac ttttctcaac ctcatcgcct
     2401 ccgagccgga catctcgcgc gtgccgctga tgatcgactc ctcgaaatgg gaagtgatcg
     2461 aggcgggcct gaagtgcatg cagggcaagc ccgtggtcaa ctcgatcagc ctgaaggaag
     2521 gcaaggacga gttcgtgaag cacgcgaagc tcgcgcgccg ctacggcgcg gcggtgatcg
     2581 tcatggcgtt cgaccagaag gggcaggccg acacgctcga gcgccgggtg gagatcgcca
     2641 agcgctgcta tcacatcctg gtggacgaac tgggctttcc gcccgaggac gtgatcttcg
     2701 acccgaacat cttcgccatc gccacgggga tcgaggagca cgcgaactac gggaaggatt
     2761 acatcgaggc cgcgcgcgtc atccggcaga cgctgccgta cgcgaaggtc tccggcggcg
     2821 tatccaacat ctcgttctcg ttccgcggca acgacccggt gcgcgaggcg atccacacgg
     2881 cgttcctcta ccatgccgtc aaggtgggca tgaccatggg catcgtcaat gccggccagc
     2941 tcggcattta tgaagaaatc ccgaaggagc tgctggagca cgtcgaggac atcctgttca
     3001 accgccggcc ggacgccgcc gaacgcatgg tcgccttcgc ggagacggtg aaggggggca
     3061 agaggcgcga caccgaggac ctcgaatggc gaaaagcggc ggtcgaggag cgcctgaagc
     3121 acgcgctggt gcagggcatc gccaactgga tcgtggagga caccgagga
//
LOCUS       DBNGWZ010000187         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2034470, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000187 DBNGWZ010000000
VERSION     DBNGWZ010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2034470"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /gene="rpsP"
                     /locus_tag="AC16CX_05495"
     CDS             complement(<1..206)
                     /gene="rpsP"
                     /locus_tag="AC16CX_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3012001.1"
                     /translation="MVVIRLARGGAKKRPFFNLVVADSRRARDGRFIERIGFYDPKAP
                     EGRERLRIDGARLTYWKGKGALLS"
     gene            801..>1600
                     /locus_tag="AC16CX_05500"
     CDS             801..>1600
                     /locus_tag="AC16CX_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3012002.1"
                     /translation="MSSPTQFAQCIEAAVAERFGSKLDVDASLPGLEELTRIAAHRSL
                     RKYDARVVEPALLRLLFACALSAPSKSDLQQADIVHVADRARVRAIADGIPDMPWIAD
                     APVFLVFCGNNRRIRQIGEWRGKPFANDHLDHFMNAAVDAGLVMMSFIRAAEAVGLGA
                     CPISAVRNHPHEVSRLLELPDWVFPVAGLCVGYPAEAGRISPRLPLEVTVHVDRYDET
                     GIRGRIDAYDRRRHALVPYRRQRYADRYPDAEFYGWSEDKARQYSVPER"
BASE COUNT          272 a          513 c          541 g          274 t
ORIGIN      
        1 tcggagagca gcgcgccttt gcccttccag taggtcaggc gcgcgccgtc gatgcgcagc
       61 cgctcgcgcc cttcgggcgc cttgggatcg tagaagccga tgcgctcgat gaagcggccg
      121 tcgcgcgcgc ggcgcgaatc ggccaccacc aggttgaaga acgggcgctt cttggcgccg
      181 ccgcgtgcca aacggattac taccatgggt tctgcctgcc cggaaaatgc ggaaagggcg
      241 tgattttacg ctagattttc gccttcacgc aaggcgaaga tgagccgcgc gtcggcgcgg
      301 cagcgagtgc cggaaagggt gacatcatcg ccgggtgggc aaggaatcct ggttttgggg
      361 cccctgagag ccgccgagca gtctcagccg agaagggggg tggctgtgac tcgttagccg
      421 caggcccgtt tgcgggagca gccgcattag acccggtcca ctgacacgtc catggcctca
      481 ggcgattctc ttaccgaacg ctattcgagc ccgatgccgg gtagtcggcg agcactgggc
      541 gagccgtgaa tgcgcccgtg gcaccccgga agattcccgc ctaatatgat tgcaataccg
      601 taagtggttc gcgagttgcg cagccgcccc gccttggcga gcagcacagg ggaccgcacg
      661 agcttgcgag gtagcggcgg tgaaccgggg cgcccttttc tttcgtgcct ttcttttggg
      721 cgcgcaaaag aaaggcacta ggccccgcag gggactgtac ccggtccgca ttgacatgca
      781 gtctattttt aaagaatccc atgagttcac caacacaatt cgcccagtgt atcgaagccg
      841 cagtcgcgga gcggttcggc tctaagctcg acgttgacgc aagcctgccc ggcttagaag
      901 aacttacccg catcgcggcg caccgctcgc tgcgcaagta cgacgcgcgc gtcgtcgagc
      961 ccgcgctgct gcggctgctc ttcgcctgcg cgctctcggc gccgtccaag tccgacctgc
     1021 agcaggccga catcgtgcac gtcgccgacc gcgcgagggt gcgggcgatc gcggacggga
     1081 ttcccgacat gccgtggatc gcggacgcgc cggtgttcct cgtgttctgc ggcaacaacc
     1141 gtcgcatccg gcaaatcggc gagtggcgcg gcaagccgtt tgccaacgat cacctcgatc
     1201 atttcatgaa cgccgcggtc gatgcgggac tcgtgatgat gagcttcatc cgcgcggcgg
     1261 aagccgtggg gctcggcgcc tgccccatca gcgcggtgcg caaccatccg cacgaggtga
     1321 gccggttgct cgagctgccc gactgggtgt ttccggtggc gggactatgc gtgggctatc
     1381 ccgcggaagc agggcgcatc agcccgcggc tgccgctgga ggtcacggtg cacgtggacc
     1441 gctacgacga gacgggcatc cgcgggagga tcgacgccta cgaccggcgg cggcacgcgc
     1501 tcgtgccgta ccgcaggcag cgctacgcgg accgctatcc cgacgcggaa ttttacggct
     1561 ggtccgagga caaggcgcgc cagtactcgg tgcccgagcg
//
LOCUS       DBNGWZ010000188         2026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3293250, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000188 DBNGWZ010000000
VERSION     DBNGWZ010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3293250"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CX_05505"
     CDS             complement(<1..230)
                     /locus_tag="AC16CX_05505"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR3012003.1"
                     /translation="MPLIRPFAGLRPASGRTAEVIAPPYDVLSSDEARARAAGKPWSF
                     LHISKPEIDLPAGTHPYAPEVYAKAAENLNLM"
     gene            complement(340..912)
                     /locus_tag="AC16CX_05510"
     CDS             complement(340..912)
                     /locus_tag="AC16CX_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012004.1"
                     /translation="MNLNPAALRAVILVIELVTFFVVTAFTAYFISHASALQLAPGEA
                     PPAQFPVIAYDGDRARPAAGNYLVVPWGEWERLAAKRPAASLLLPERSRTVRIGDAGE
                     ASFTVTEEAGSRQTVELTWRTGGGEQQTRYVAQARSIEPRYLRTLSAQTLLMAAAAGF
                     LAGLFTGRALRRRWLAQPGYFVPPPQQGQG"
     gene            complement(977..>2026)
                     /locus_tag="AC16CX_05515"
     CDS             complement(977..>2026)
                     /locus_tag="AC16CX_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HIR3012005.1"
                     /translation="SRPLIREAALRELKRGGLVYFLHNDIDTIHHVEASLVKLLPEAR
                     IRVAHGRMRERELEHVMRDFYQSRFNLLLCTTIIETGIDVPAANTIIINRADKFGLAQ
                     LHQLRGRVGRSHHQAYAYLLLPDEGNITAAARKRLEAIQMMEQLGSGFFLAMHDLEIR
                     GAGEVLGEAQSGEMLEVGFNLYVRMLDAAVRSLKTGREPDLAAPLEVNTEINLHVPAL
                     LPADYCSDVHERLVLYKRLANCETLETLDALREELADRFGPLPEAARSLIECHRLRVL
                     GRPLGVARIDASDSAIQLQFVAKPPIEPIKVLDLVHRKRHYRLAGQDRLRIELKLPDV
                     KTRVAAIREAFGELS"
BASE COUNT          354 a          637 c          692 g          343 t
ORIGIN      
        1 aacatgagat tcaggttttc cgctgccttg gcatagactt caggcgcata gggatgggtt
       61 cccgccggca ggtcgatctc cggcttggaa atgtgcagga agctccatgg cttgccggcg
      121 gctcgcgcgc gcgcttcgtc gctggacagc acatcgtagg ggggagcgat gacttcggcg
      181 gtgcggccag aagccgggcg cagtccggcg aagggtcgga tcaggggcat ggcttatcct
      241 aaggcagaag gaggaaggat gaaggatgaa ggatgaaggg tgagagagga cccccgcagt
      301 gcgggtatga aaaccaggtc atccttctgc cttctgcctt catccttgcc cttgttgtgg
      361 gggagggacg aagtaacccg gctgcgccag ccaccgccgc cgcaacgccc ttcccgtgaa
      421 caggccggcg aggaagccgg cggcagcggc catcaacagg gtttgagcgc ttagcgtccg
      481 caagtaacgc ggctcgatgc tgcgtgcctg cgcgacgtag cgggtctgct gctctccgcc
      541 gccagtccgc catgtcagct ccacggtctg ccgtgaaccg gcttcctcgg tgacggtgaa
      601 cgatgcctcc cccgcatcgc cgatccggac ggtacgggaa cgctcgggaa ggagcaggct
      661 cgcagcgggc cgtttcgcag cgagtcgttc ccattcgccc cacggaacga ccaggtaatt
      721 cccggccgcg ggccgcgccc ggtctccatc gtaggcgatc acgggaaact gcgcgggcgg
      781 cgcctcgccc ggcgccagtt gcagcgccga ggcatgcgag atgaagtagg cggtgaacgc
      841 cgtcaccacg aagaacgtga ccagctcgat gacgagtatg acggcgcgca acgcggctgg
      901 attcaggttc attgccggag tgcaaggaaa gcggcggact atgataccca atcggaggcc
      961 gtcatccgtc gacccgtcaa gagagctcgc caaacgcctc gcggatcgcc gcaacgcggg
     1021 tcttgacgtc gggcagcttc agctcgatgc gcaaacggtc ctggcccgcg aggcggtaat
     1081 gccgcttccg atgcacgaga tccagcacct tgatcggctc gatcggaggc ttcgccacga
     1141 actggagctg gatcgcggaa tcgcttgcgt ctatgcgcgc gacgccaagc ggtcttccga
     1201 gcacgcgcag gcggtggcac tcgatgagcg accgtgcggc ctcgggcagc ggcccgaaac
     1261 ggtcagccag ctcctcgcgc agggcatcga gcgtctccag tgtttcgcaa ttggcgagcc
     1321 gcttgtagag caccagtcgc tcgtgcacgt cgctgcaata atccgccggc agcagtgccg
     1381 gcacgtgcag gttgatttcg gtgttgacct cgagcggcgc ggcgaggtcg ggctcgcgcc
     1441 cggtcttcag cgagcgcacg gcggcatcca gcatgcgcac gtagagattg aagcccactt
     1501 caagcatctc cccgctctgg gcctcgccca ggacctcgcc ggcgccgcgg atctccagat
     1561 cgtgcatggc gaggaagaaa cccgagccca gctgttccat catctggatc gcttccagcc
     1621 gcttcctcgc ggctgcggtg atgttgccct cgtcgggcag cagaagatag gcgtaggctt
     1681 ggtgatgcga gcggccgacg cgcccgcgca gctggtgcag ctgcgcgagc ccgaacttgt
     1741 cggcgcggtt gatgatgatg gtgttggcgg ccggcacgtc gatgccggtc tcgatgatgg
     1801 tggtgcacag cagcaggttg aagcggctct ggtagaaatc gcgcatcacg tgctcgagct
     1861 cgcgctcgcg catccgaccg tgagcgacgc ggatccgcgc ctccggcagc agcttcacga
     1921 gcgacgcctc gacgtgatgg atcgtgtcga tgtcgttgtg caggaagtaa accaggccgc
     1981 cgcgcttcag ctcccgcagc gcggcctcgc ggatgagcgg cctgct
//
LOCUS       DBNGWZ010000189         3961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_773883, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000189 DBNGWZ010000000
VERSION     DBNGWZ010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_773883"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC16CX_05520"
     CDS             <1..323
                     /locus_tag="AC16CX_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease family protein"
                     /protein_id="HIR3012006.1"
                     /translation="PGKRNSLDALCERYQIDNSARTLHGALLDARLLAEVYLAMTRGQ
                     ESLVMEVETSAATATAAETVAGKLDLVVIRATGDELAAHKQLLADIDKASDGRCLWRK
                     LEKA"
     gene            507..1121
                     /gene="cysC"
                     /locus_tag="AC16CX_05525"
     CDS             507..1121
                     /gene="cysC"
                     /locus_tag="AC16CX_05525"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3012007.1"
                     /translation="MSKSPNAVWHHATVTRDRREKLQGHCGVVLWFTGLPSSGKSTIA
                     HTVEERLHQMGCRTFVLDGDNVRHGLCGDLGFSGKDREENIRRIGEVTKLFVEAGTIV
                     LTAFVSPYRRDRERARGLLLHGDFLEIYCRCPVEICEQRDPKGYYQRAKEGRIKEFTG
                     VSAPYEEPGEPELVLDTAKLSIEESVEVVLGLLRNRGIIKPGNA"
     gene            1555..2415
                     /gene="nudC"
                     /locus_tag="AC16CX_05530"
     CDS             1555..2415
                     /gene="nudC"
                     /locus_tag="AC16CX_05530"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR3012008.1"
                     /translation="MPEYIPLVIPPERLTEPVLWFAFRRAEILVLNGADQPALPCCVD
                     LREHGFEPQRNQYLGLYGGKHCYAVEIHESQPLPQGWSALGLRDLFGLVETTLAALSG
                     RAYQILEWDRNHRYCSRCGTPMQPRTDERARACPACGFTSYPPVTPAIMILVTHGRKL
                     LLARKPAFPPGRFSALAGFVEPGETLEDTVIRETREEVGVEVKNIRYFGSQPWPFPHS
                     LMIAFTAEYAGGEVRPDGVEIEEVHWFEPDRLPKLPPPISISRRLIDTIAGRMVRGDP
                     IGGSRERRDS"
     gene            complement(2524..3942)
                     /locus_tag="AC16CX_05535"
     CDS             complement(2524..3942)
                     /locus_tag="AC16CX_05535"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR3012009.1"
                     /translation="MLIPVILSGGAGARLWPVSREAFPKPFIRLADGSSLLRKTLARA
                     GRINGVSHVVTVTNREYYFLTKDQYSEFGGLSHVFLLEPAARNTAPAIAMAAFHIAAR
                     HGAETELLVLPADHLIEDEAAFVSAVEIARSLSRKGSLVTFGIPPSRPETGYGYIECG
                     QAIDGKGSCKVARFVEKPGAEKAKAYLATGRFLWNSGMFCFRAGAFLEALRKCDGMLY
                     KKALASWEATPPPSEDKINLDPERFAALPDISVDYAVMEKHPDVVVVKATFDWNDIGS
                     WNALSALTPPDEHGNRTQGETVLMDARDCYIQSDSRVVAAVGVENLVVVDTPDALLVA
                     SKDRVQDVKQVVQRLKLTNHSAHLLHRTVHRPWGTYTVLEEGAGYKIKRIVVKPGASL
                     SMQMHHHRSEHWVVLAGTAKVVNGDKELLIQPNESTFIPVGNKHRLANPGTDDLVIIE
                     VQAGSYVGEDDIVRFDDVYGRT"
BASE COUNT          730 a         1259 c         1226 g          746 t
ORIGIN      
        1 accccggcaa gcgcaacagc ctcgacgccc tgtgcgagcg ctatcagatc gacaactccg
       61 cccgcacgct gcacggcgcg ctgctggatg cgcggctcct cgcagaggtc tatcttgcga
      121 tgacgcgcgg ccaggagagc ttggtgatgg aggtggaaac ttcagcggca accgcgaccg
      181 ccgccgaaac cgtcgcaggc aagctcgacc tggtggtcat ccgcgcgacc ggcgacgagc
      241 ttgctgccca caagcagctg ctagccgata ttgataaagc gagcgatggt cggtgcctct
      301 ggcgcaagct cgaaaaggcg tgagtagtga atggtgagta gtgagtggac tgttctaagc
      361 agccgcagct gtctgcttga ccgaccttcg caggccaacc agtaatttcc ccacccgctc
      421 gagcttcaag caattaacgg gcttgaggtg cagctggttt acaatccgtc cactcaccac
      481 tcattactca ccactcacgc atcttcatga gcaagagtcc caacgccgtc tggcaccacg
      541 ccaccgtcac ccgcgaccgg cgcgaaaagc tccagggcca ctgcggcgtc gtgctgtggt
      601 tcaccggctt gcccagctcc ggcaagtcca ccatcgcgca cacggtcgag gagaggttgc
      661 atcagatggg gtgccgcacg ttcgtgctcg acggggacaa cgttcgccac ggcctctgcg
      721 gcgatcttgg tttttccggg aaggaccgcg aggagaacat ccgccgcatc ggcgaggtga
      781 ccaagctttt cgtggaggcg ggcacgatcg tattgacggc gttcgtgtcc ccctatcgcc
      841 gcgaccgcga gcgcgcccgc ggattgctgc tgcatggtga tttcctcgag atttactgcc
      901 gctgcccggt cgaaatctgc gagcagcgcg accccaaggg gtactatcaa cgcgcgaagg
      961 aaggcaggat caaagaattc accggtgttt ccgcgccgta cgaagagccg ggcgagccgg
     1021 agctggtgct ggacaccgcg aagctatcca tcgaggaatc ggtggaagtg gttctgggct
     1081 tgctacggaa ccgtggcatc atcaagcccg gtaatgcgtg acatggtgat gcgtgacatg
     1141 gtgatgcgtg acgcgataat tcgtgacatg atgaggcgag ttttacacca ccccgcctgc
     1201 ttcgccggca ccccgcccgg ccaaccgcac ggaggcggtt ggcgttcggc ggctcaccct
     1261 cacccccgcc cctctccata aataataagg gcgaggggag atttgagatg cgcttttcac
     1321 cagggaaaag cgcatggaaa aaggatggaa accccgcctc accctcagac aggaggggaa
     1381 ttttgttaat ccccctcttt cggaggaggg gtggcgccga aggcaccggg gtggtgggaa
     1441 tcgaattacg attcacgaat cacaactcac ggggttcccg gtcacgcagt aacacgtcac
     1501 gcggtatcat gtcacgccgt atcatgtcac gccgtatcac gtcacgcctt gccaatgcct
     1561 gaatacatcc ccctcgtgat ccccccggag cggttgacgg agcccgtgct gtggttcgcg
     1621 ttccggcgcg cggaaatcct ggtgctgaac ggcgcggacc agcctgcctt gccgtgttgc
     1681 gtggatttac gggagcacgg cttcgagccg caacgcaacc agtacctcgg gctctacggc
     1741 gggaagcact gctacgcggt cgagatccac gaatcccagc cgctgccgca aggctggtcc
     1801 gccctcgggc tccgcgacct gttcggcttg gtggaaacga cgctggcggc gctgtctgga
     1861 cgcgcctacc agatcctcga atgggatcgc aatcaccgct actgcagccg ctgcggcacg
     1921 cccatgcagc cgcggacgga cgagcgcgcg cgcgcctgtc ccgcctgcgg cttcacgagc
     1981 tacccgccgg tcacgcccgc catcatgatc ctcgtcacgc atggccggaa gctgctgctc
     2041 gcgcgcaagc cggcgttccc gccgggacgg ttctccgcgc tggcgggctt cgtcgagccg
     2101 ggcgagacgc tggaggacac cgtcatccgc gagacgcgcg aggaagtcgg ggtggaggtg
     2161 aagaacatcc gctacttcgg cagccagccc tggccgtttc cgcattcgct gatgatcgcg
     2221 ttcaccgcgg aatatgccgg cggagaggtc cggccggacg gggtggagat cgaagaggtg
     2281 cactggttcg aacccgaccg gcttcccaag ctcccgcccc cgatcagcat ctcgcggcgc
     2341 ctcatcgata ccattgcagg ccgcatggtc cgcggtgatc cgatcggcgg atcgcgggaa
     2401 agacgtgatt cgtgaatcgt gattcgctaa cttaagttac aagttccaag cttcaagtta
     2461 caagacaagc ccttgttact tgtaccttgt tgcttgtgac ttgtgtcttg taacttgtaa
     2521 ctttcaagtg cggccgtaaa cgtcatcgaa ccggacgatg tcgtcttccc caacgtagct
     2581 gccggcctgg acttcgatga tgaccagatc gtccgtaccg ggatttgcca gccggtgctt
     2641 gttgcccacg gggatgaagg tggactcatt cggttgaatc agcagttcct tgtcgccgtt
     2701 gacgaccttg gccgtcccgg cgagcaccac ccagtgctcg ctgcggtggt gatgcatctg
     2761 catgcttaac gaggctcccg gcttcaccac gatgcgcttg atcttgtagc ccgcgccttc
     2821 ctcgagcacg gtataggtgc cccacggccg gtgcacggtg cggtgcagca ggtgggccga
     2881 atgattggtc agcttcagcc gctgcaccac ctgcttgacg tcctgcaccc gatccttgct
     2941 ggcgacaagc agcgcatcgg gcgtatcgac caccaccaga ttttcgaccc cgaccgcggc
     3001 gacgacccgc gagtcgctct ggatgtagca atcccgcgca tccatcagca cggtttctcc
     3061 ttgcgtgcgg ttgccatgct cgtccggcgg agtgagcgcg ctcagggcat tccacgagcc
     3121 gatgtcgttc cagtcgaacg tggctttcac gaccacgacg tccgggtgct tctccatcac
     3181 cgcgtagtca acggagatgt cgggcagtgc ggcgaagcgc tccgggtcga ggttgatctt
     3241 gtcctcggag ggcgggggtg tcgcttccca gcttgccagc gccttcttgt agagcatccc
     3301 gtcgcatttg cggagggcct ccagaaaagc ccccgccctg aagcaaaaca tgccggaatt
     3361 ccacagaaac ctgcccgtgg cgagataagc cttggctttc tcggcaccgg gtttctcgac
     3421 gaatcgggcg accttgcaac tgcctttgcc gtcgatcgcc tgcccgcact cgatgtagcc
     3481 atacccggtt tcgggccgtg atggcgggat gccgaacgtc acgagcgatc ccttccgtga
     3541 aaggctgcgg gcgatttcca cggcggacac gaacgccgcc tcatcctcga tcagatgatc
     3601 cgccggcagg accaggagct cggtttccgc gccgtgcctg gcggctatgt gaaacgccgc
     3661 catcgcgatc gccggggccg tgttgcgggc ggcaggctcc agcaggaaca cgtgagagag
     3721 gccgccaaac tcggaatact ggtccttggt cagaaagtag tactcgcggt tggttacggt
     3781 caccacgtgc gacacgccgt tgatccgccc ggcgcgcgcc agcgttttcc gcagcaggct
     3841 gctgccgtcc gcgaggcgga tgaacggctt cggaaaggcc tcgcgcgaca cgggccataa
     3901 acgggcgccc gccccgccgg agagaatgac tggaatcagc atcgtctggt ttcaggaatc
     3961 g
//
LOCUS       DBNGWZ010000190        11434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2916918, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000190 DBNGWZ010000000
VERSION     DBNGWZ010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2916918"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..394
                     /locus_tag="AC16CX_05540"
     CDS             230..394
                     /locus_tag="AC16CX_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3012010.1"
                     /translation="MRDDKGTPDLVTRTRDFALRVIKFYSGLPFETVAQVLGKQLLRS
                     GTSVETKAHK"
     gene            complement(426..2000)
                     /gene="gshA"
                     /locus_tag="AC16CX_05545"
     CDS             complement(426..2000)
                     /gene="gshA"
                     /locus_tag="AC16CX_05545"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HIR3012011.1"
                     /translation="MHKRLRALAPEVLRSLRRGIEKESLRVRLDGKLAGTPHPAALGS
                     ALTHPHITTDFSESQLELITGVHTGPEACIEEITAIHQAVYRHIGDEMLWCASMPCGL
                     PADDDIPVGRYGSSNIGRAKTVYRIGLSHRYGRRMQTISGVHYNFSLPDAAWPMLQRA
                     DGRGGPAQAYRNEAYFSLIRNFRRHSWLLLYLFGASPAVCASFVAGRKHRLQELKAGT
                     LYLPGATSLRMGPLGYQSEAQASLAVSYNSLESYARSLHHALTEPYPPYAAIGIRDGA
                     DYRQLATTLLQIENEFYGTIRPKRTIRTGERPLHALGERGVEYVEVRCVDLDPFSPVG
                     ITAATMRFLDVFLLHCLLSDSPADTPAEIAALHRNQHRAAERGREPGLRLERRGEEIE
                     LAQWGAEVLRECEPIAAALDAAHRVTAYSEALSAAGAALANPSATLSARMLREMEEAC
                     DNSYLGFAMARSTRYRGELLARPFPAEMDTRYRRLAEDSLAKQREIEAKDTMPFETFR
                     QEYLSRDLLSGLRLEG"
     gene            2096..3073
                     /locus_tag="AC16CX_05550"
     CDS             2096..3073
                     /locus_tag="AC16CX_05550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3012012.1"
                     /translation="MQPAPYSGLTPDRALDALAAVGLRPDGRMLALNSYENRVYQVWM
                     EEGAPLVAKFYRPARWSDAQIQEEHAFAQELAEQEIPVVAPLAIENRTLHAFEGFRYA
                     VFPRRGGRPPELEDRKVLEWIGRFVGRIHAVGAIRPFHERPALDIESHGAEPREWLLA
                     SGLVPADLLEAWASTTALALDAVRRSYERARDVRLIRLHGDCHAGNVLWTDAGPHFVD
                     FDDCRTGPAAQDLWMLLSGDRAAMTRQLLDVLAGYEDFRDFDRRELQLVEALRTLRLI
                     HYSAWIARRWDDPAFPAAFPWFGTHRYWQDRILETREQIAAMDEEPLSV"
     gene            3282..3575
                     /locus_tag="AC16CX_05555"
     CDS             3282..3575
                     /locus_tag="AC16CX_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3012013.1"
                     /translation="MPKAYWVACYRSIRNPEALAAYAKLAGPAIQAAGGRFIVRGTPA
                     KSYEAGLNQRVVVVEFDSVTQAIAAHDSAAYQAALKALGNAAERDIRIVEGVG"
     gene            3740..4474
                     /locus_tag="AC16CX_05560"
     CDS             3740..4474
                     /locus_tag="AC16CX_05560"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR3012014.1"
                     /translation="MRYELYYWPHIQGRGEFIRLALEEGAADYVDVARMPESEGGGRT
                     AVSRLLEPAGDGRTPFAPPILKAGKLLISQTANILFYLGPRLGLVPKTEAARLWANQL
                     QLTLADLLVECHDTHHPIAKSLYYEEQKPEALRRAADFKTLRLPKFLGYFERVLAANP
                     GGDRHLIGKSLTYVDLSMFQMIAGLNYAFPRTMARLKSGHPRLTALHQRVTERPRIAA
                     YLASSRRIPFNERGVFRYYSELDEGS"
     gene            4471..4917
                     /locus_tag="AC16CX_05565"
     CDS             4471..4917
                     /locus_tag="AC16CX_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012015.1"
                     /translation="MSAITPNGTHHANLPPLASRFVNVTALPWEKTRHPGVETKALVA
                     DPGTGLLTVLTRMAPGAKLPDHEHVLIEQTYVLDGTLHCGEGVCRAGNFVWRPAGSRH
                     EAWAGPEGCLALAMFQVPNRFYEPDGRVVDFLGNDWEKTWGALFTR"
     gene            5122..6741
                     /locus_tag="AC16CX_05570"
     CDS             5122..6741
                     /locus_tag="AC16CX_05570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3012016.1"
                     /translation="MRSLGPFDYVIVGAGTAGCVLANRLSANPDVSVLLIEAGGKDDW
                     IWIHIPVGYLYCIGNPRTDWRYKTEPEPGLNGRSINYPRGRVLGGSSSINAMLCLRGQ
                     ARDYDEWAGITGDSGWAWRNVLPVFIKVEDHWRGADEKHGQGGEWRVETPRARWEIIE
                     AFRDAAIEAGIPWTEDFNRGDNEGVSRFEVNQKNGVRWNASKAFLRPAMKRPNLTVVT
                     GALVKKLRLEGRRALGVEFDQAGEAVFAEARRETILSAGAIGSPQILQLSGIGPGALL
                     QKHGIPVAHELPVGENLQDHLQLRLAFKVKNALTLNTMLGSWWSKAKIGLEYLLFRTG
                     PMTMPPSQLGAFVKSDASQPTPNLEYHVQPISLDKFGDPPHPFPAFTASVCNLRPTSR
                     GHVRITSPDPRAYPAITCNYLSTNEDRKTAAAAIRLTRRIVTGTRALKRYEPEEFMPG
                     PAFQSEEELVRAAGDIGTTIFHPVGTCKMGRESDPSAVVDPRLRVRGVERLRVIDASI
                     MPTITSGNTNTPTVMIAERGSALVRGKDGRD"
     gene            6888..7814
                     /locus_tag="AC16CX_05575"
     CDS             6888..7814
                     /locus_tag="AC16CX_05575"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012017.1"
                     /translation="MLALFSATAAAQAYPQRPIRLLVPNPPGGATDNLARVVAPKLTE
                     LLGQTVVVDNRPGAAGNLAAEAAARATPDGYTLMLAADGQIVLSPHLYRKLPFDTLKD
                     LTPVASLVSTQMLLAVHPALPVKTVPEFVEYARRASLPLAYGSIGNGSQHHLAMEMLK
                     RRADINLLHVPYKGGGPALVGLFGGEVSVMFGGSSAGPHVRSGKLRALALAGKRNPAY
                     PGLPALSDTYPGLEVTPWLGLFAPVGLPATALNRLRTETGRMLADPDSRARVGNIALD
                     PFPTTPQEFSALIRADYAKYGEIIRSVGVKID"
     gene            complement(7902..8261)
                     /locus_tag="AC16CX_05580"
     CDS             complement(7902..8261)
                     /locus_tag="AC16CX_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019366.7"
                     /note="extradiol catechol dioxygenase that catalyzes the
                     oxidative cleavage of substituted catechols; part of the
                     bacterial aromatic compound degradation pathway; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HIR3012018.1"
                     /translation="MNPQLEGVEALEGTYLFTLERSARALRLNRFLHGLTVPEKRGLF
                     KQDAESALEKAGLDAEERRMVRELDWAALIRYGASFFCLEKLARVKGVSNPEMVASFR
                     GETLEQFLKTRNVPGAR"
     gene            complement(8348..9313)
                     /locus_tag="AC16CX_05585"
     CDS             complement(8348..9313)
                     /locus_tag="AC16CX_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012019.1"
                     /translation="MFRIAAGCLLPAFLAVAPAWPQQYPVKPVRIIAPFAPGGTADTL
                     GRIVAAKLTESLGQNFIVENRPGAGGLLGSELVSKSAPDGYTLVVSGVASHAIAPALA
                     AKPPFDPVKDFTHIALFGGPPAVFAAHPSLPVKDLKGFVAFAKARPRNVTYGSPGVGT
                     QGHLVAELFKQKSGIDIVHVPYKGAAIAVVDIVAGHIHAISTTLTTASAQIRAGRVRA
                     LALSSANRLPDYSGVPTFRELGYRDLVATVWFSLSGPAGVPADVVNRLNSEVRRILQL
                     PDVRERLRGDGIEPNQLDAKAFTDYMTAEVKRWGPVVRASGARTD"
     gene            complement(9370..10344)
                     /locus_tag="AC16CX_05590"
     CDS             complement(9370..10344)
                     /locus_tag="AC16CX_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012020.1"
                     /translation="MVRQRLRFLCVFATAAWLGSAAAQDFPTRPIRVVIPYAAGGLPD
                     VMTRIASQRMIETLGQQIVVDNRPGAGGIGACELVAKSNPDGYTLLITDVAQTAINPA
                     LYTKLPYDTLRDFAPVSLMGISGQFLVAHASVPANTLPELIKLVKSKPGQFRYGSGGT
                     GSVHHLSMEALKTPLGLDIVHVPYKGTGQAVPALLAGEVSLLFAALPSIAPHVKAGRL
                     KLLAVNTIRRNAQAPEVPTIAEITGIKDYDYPPAIGVLAPAKTPKAIVNRLASAIVKA
                     VQHPDIVNRYTTLGIDPVGNTPEQYAAQIRIDIAKYARAVKASGVKVD"
     gene            10433..11134
                     /locus_tag="AC16CX_05595"
     CDS             10433..11134
                     /locus_tag="AC16CX_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3012021.1"
                     /translation="MSVKGLIDDLVSANHILFKEGVVDGFGHVSVRDGRNPERFLLAK
                     SIAPATVKARDIMEFGMDGAPLNPRGRKPYLERYIHSEIYRARPDVTAVVHSHSPALI
                     PFGITGTSLHPVFHMSGFLGSATPIFEIREAGGPATDMLIRSRELGAALARTLGPAPF
                     ALMRGHGSVAVGATLKQVVYRAIYAEVNARLQSEAMRLGKINFLNDEEAANASRTNDG
                     VIDRPWELWKKRARE"
BASE COUNT         2010 a         3701 c         3812 g         1911 t
ORIGIN      
        1 ggaaggcgag gatgaggccg tcacgtccgc gctgctgaag gtcggctctc tcggaaacgt
       61 gcgcagcgaa accctgcgcg cattctccgc ggcgcagatg ggccagatcg tcggcaacat
      121 tccctgacct acggaaggaa gaaggaagaa ggaggaggac aacaagacgc cggtcaacgg
      181 ccagctagtt cgggcgttac tgtcgagcaa gggatgccag gagcccgaca tgagggacga
      241 caagggaacg ccggaccttg taacccgcac aagagatttc gctcttcgtg tgatcaagtt
      301 ctattccggt cttccgtttg agaccgtggc tcaggtgttg ggcaagcagt tgctgcgttc
      361 gggtacgtcc gtcgagacga aggcgcataa gtaatttcgc aggcatttcc ttcatccttc
      421 tgctttcatc cttccagccg caggccgctc agcaggtccc gcgaaagata ctcctggcgg
      481 aaagtctcga agggcatagt gtccttcgct tcgatttcgc gctgcttggc gagcgagtcc
      541 tcggccaagc gccggtagcg cgtatccatt tcggctggaa acggccgcgc gaggagttcg
      601 ccgcggtagc gcgttgagcg cgccatggcg aaaccgagat aggagttgtc gcaggcctcc
      661 tccatttccc ggagcatgcg ggcggagagc gttgccgagg gattcgcgag cgcggccccg
      721 gcggctgaca gggcttcgga gtacgcggtg acgcggtggg cggcgtcgag cgccgccgcg
      781 atcggctcgc attcgcgaag cacctccgcg ccccattggg ccagctcgat ctcttctccc
      841 cggcgctcca gccgcaggcc cggctcccgg ccgcgctcgg cggcccggtg ctggttgcgg
      901 tggagcgcgg cgatttcagc cggcgtgtcg gcggggctgt cgctcaacag gcagtgcagc
      961 aggaacacgt cgaggaaacg catcgtggcg gcggtgatgc cgacgggcga aaacggatcg
     1021 aggtccacgc agcgcacttc gacgtactcg acgccgcgct cgcccagcgc gtgcagcggc
     1081 cgctcgcccg tgcggatggt gcgcttcggc cggatggtgc cgtagaactc attttcgatc
     1141 tgcaacagcg tcgtggcgag ttgccggtag tccgcgccgt cgcggatgcc gatggcggca
     1201 tacggcgggt agggctcggt gagcgcgtgg tggagcgagc gcgcgtagct ctccaggctg
     1261 ttgtagctca ccgcaagcga ggcctgcgcc tcgctctggt agccgagcgg gcccatgcgc
     1321 agcgaggtcg ctcccggcaa atacagcgtg ccggccttga gctcctgcag ccggtgcttg
     1381 cgcccggcga cgaagctcgc gcacaccgcg ggcgatgcgc cgaacagata gagcagcagc
     1441 caggagtggc gccggaagtt gcggatgagc gaaaaatacg cctcgttccg gtaggcctgc
     1501 gccggtccgc cgcgcccatc ggcccgctgc agcatcggcc acgcggcgtc gggcagggag
     1561 aagttgtagt gcacgccgga gatcgtctgc atgcgccgcc cgtagcgatg cgacaggccg
     1621 atgcggtaca ccgtcttcgc cctgccgatg ttcgagctgc cgtaacggcc gacgggaatg
     1681 tcgtcatccg cgggcaggcc gcaaggcatg ctggcgcacc acagcatttc atcgccgatg
     1741 tgccggtaga cggcctggtg gatcgcagtg atctcctcga tgcacgcctc cggcccggtg
     1801 tggacgccgg tgatgagctc gagctgcgat tcgctgaaat cggtggtgat gtgcggatgg
     1861 gtgagcgccg agccgagcgc cgccgggtgc ggcgtgcccg cgagctttcc gtcgagcctc
     1921 acgcggaggc tttccttctc gatgccgcgc cggaggcttc tcagcacttc gggcgcgagc
     1981 gcgcgcaggc gtttatgcat cacgtgggcc aggccgatac ccgtccgcta ttccggctcg
     2041 atcatggcag tctagctcaa gcggtgactc gtcgcgtatt gactacaatg ggcgggtgca
     2101 gcccgccccg tactccggcc tcacgccgga ccgcgcgctc gacgccctcg cagcggtcgg
     2161 ccttaggccc gacgggcgca tgctcgcgct gaacagctac gagaaccgcg tctaccaggt
     2221 ctggatggag gaaggggcgc cgctggtggc gaagttctac cgcccggcgc gctggagcga
     2281 cgcgcagatc caggaggagc acgcatttgc gcaggagctc gccgaacaag agatcccggt
     2341 agtcgcgccg ctcgcgatcg aaaaccgcac gctgcacgcg ttcgagggtt tccgctacgc
     2401 ggtctttccc cggcgcgggg gtcggccgcc cgagctggaa gacaggaagg tgctcgaatg
     2461 gatcgggcgc ttcgtcggcc gcatccacgc ggtgggcgcg atacggcctt tccacgagcg
     2521 gcccgcgctc gacatcgaaa gccacggcgc cgagccgcgc gagtggttgc tcgcgagcgg
     2581 tctcgttccc gcggacctgc tcgaggcatg ggcgagcacg acggcgctcg cgctcgacgc
     2641 cgtgcgccgc agctacgagc gcgcaagaga cgtgaggctg atccgccttc acggcgattg
     2701 ccacgcaggc aacgtgctgt ggacggacgc gggcccgcac ttcgtggatt tcgacgactg
     2761 ccgcacgggg cccgcggcgc aggacctgtg gatgctgctc tcgggcgacc gggcggcgat
     2821 gacccggcag ctcctggatg tgctcgcggg ctacgaggat ttccgcgatt tcgaccggcg
     2881 cgagctgcag ctcgtcgagg cgctgcgcac attgcgcctc atccactact cggcgtggat
     2941 cgcgcggcgc tgggacgatc ccgcgttccc ggccgcgttt ccctggtttg gcacacaccg
     3001 ctactggcag gaccgcatac tcgagacgcg cgagcagatc gcggcgatgg acgaggagcc
     3061 gctcagcgtc tgaagcccgt tccaacctgt gcctcatggc ttcatcgccc cggtatatgc
     3121 gagcgaactg attaggacct gcgaaccctc gttggaacgg gctgaagacg gcgggatgaa
     3181 aggtggaaat tcgacggtca ccgcgagccg cctgctgtaa actgcaaacc cggattgtcg
     3241 atccgcgacc cacgattttc tactgacagg aggaaccgct catgccaaag gcctattggg
     3301 tagcgtgcta ccgctcgatc aggaacccgg aagcattggc cgcctacgcg aagcttgccg
     3361 gacccgccat acaggcggcc ggcggaagat tcattgtccg aggcacgccc gcgaaatcct
     3421 atgaggcggg gctcaaccag cgcgtggtcg tggtcgagtt cgacagcgtc acgcaggcga
     3481 tcgccgcgca cgacagtgca gcctatcagg ctgccctcaa ggctctcggg aacgcagccg
     3541 agagagacat ccggatcgtg gaaggagtcg gttgagagtt ggcagttggc agccgtcagc
     3601 tggcagcgac tgcgaactgc aaactgcaaa ctgctaactg ttcgctgcac actgcccact
     3661 gcaaactgca gacctggatt gtcgaatccc gacccactat ccgtaaccca cgatccacga
     3721 cctacgccgt tccaaaccta tgcgctacga actctactac tggccgcaca tacaagggcg
     3781 cggcgaattc atccgcctcg cgctggagga gggcgccgcc gactacgtgg acgtcgcccg
     3841 gatgcccgag agcgaaggcg ggggccggac ggcggtcagc cgcctcctcg aacccgccgg
     3901 cgacgggcgg acgccgttcg cgccgccgat cctcaaggcc ggcaagctgc tgatctcgca
     3961 gaccgccaac atcctgttct atctcggccc gcggctgggc ctcgtgccga agacggaagc
     4021 ggcgcggctc tgggcgaacc agcttcagct cacgctcgcc gaccttctgg tcgagtgcca
     4081 cgatacgcat cacccgatcg cgaagagcct ctactacgag gagcagaagc cggaggcact
     4141 gcggcgcgcg gcggatttca agaccctccg cctgccgaag ttcctcggct attttgagcg
     4201 cgtgctcgcg gccaacccgg gcggcgaccg ccacctgatt ggaaaatcat tgacctacgt
     4261 ggacctctcg atgttccaga tgatcgccgg gttgaattac gcctttcccc gcacgatggc
     4321 ccgcctgaag tccggccacc cgcgattgac ggcactgcac cagcgcgtca cggagcgtcc
     4381 gcgcatcgcc gcgtacctgg cgtccagccg ccgcatcccg ttcaacgagc ggggagtctt
     4441 ccgctactat tccgaactcg acgagggatc atgagtgcaa tcacgccgaa cgggacccat
     4501 catgccaacc tgccgccgct ggcttcacgc ttcgtgaacg tcacggcgct gccgtgggag
     4561 aagacgcgtc acccgggcgt cgagaccaag gcgctcgtgg cggatcccgg aaccggcctg
     4621 ctcaccgtgc tcacccggat ggcaccggga gcgaagctgc cggaccacga gcacgtgctt
     4681 atcgagcaga cctatgtgct cgatggcacg ctccactgcg gtgaaggcgt atgcagggcg
     4741 ggtaacttcg tctggcgccc ggcgggcagc cgccacgaag cgtgggccgg accagagggc
     4801 tgcctggcgc ttgcgatgtt ccaggtcccg aaccgcttct acgagccgga cggacgggtc
     4861 gtggacttcc tcggcaacga ctgggaaaaa acctggggcg cgctcttcac ccgctaggcg
     4921 aagagcgagg gatgaggagc tgaggcagaa ggcagaaggc agaaggcaga aggcagaagg
     4981 cagaaggcag aaggcagaag gcagaaggca gaaggcagaa ggcagaaggc agaaggcaga
     5041 aacaagtcct ggttaacgat ttttcccttc ttccttcatc cttcttcctt catcctttga
     5101 ggtatccttc atccttgagc catgcgaagt ctcggccctt tcgactacgt gatcgtcggc
     5161 gcgggcacgg cgggatgcgt gctcgccaac cgcctctccg ccaacccgga cgtctcggtg
     5221 ctgctcatcg aggcgggcgg caaggacgac tggatctgga tccacattcc ggtcggctat
     5281 ctctactgca tcggcaaccc gcgcaccgac tggcgctaca agaccgagcc cgagccgggg
     5341 ttgaacggcc gctccatcaa ctatccgcgg ggccgggtgc tcggcggctc gtcctcgatc
     5401 aacgccatgc tgtgtctccg cggccaggcg cgcgactacg acgaatgggc gggcatcacc
     5461 ggcgattccg gctgggcgtg gcgcaacgtg ctgccggtct tcatcaaggt cgaggaccac
     5521 tggcgcggcg cggacgagaa gcacgggcag ggcggcgagt ggcgcgtcga gacgccgcgc
     5581 gcgcgttggg aaatcatcga agccttccgc gacgcggcga tcgaggcggg catcccctgg
     5641 accgaggact tcaaccgcgg cgacaacgag ggcgtgtcgc ggttcgaagt gaaccagaaa
     5701 aacggcgtgc gctggaacgc gtcgaaggcg tttctcaggc ccgcgatgaa gcggcccaat
     5761 ctcaccgtgg tgaccggcgc gctggtgaag aagctgcggc tcgaaggacg ccgcgcgctg
     5821 ggcgtggagt tcgaccaggc cggcgaggcg gtattcgccg aggcgcggcg cgagaccatc
     5881 ctctcggccg gcgcgatcgg cagtccccag atcctgcagc tctccggcat cggcccgggg
     5941 gcgttgctcc agaaacacgg cattcccgtt gcgcacgagc tcccggtggg cgagaacctc
     6001 caggaccacc tgcagctgcg gctcgcgttc aaggtgaaga acgcgctcac gttgaatacg
     6061 atgctcggct cgtggtggag caaggcgaag atcggcctcg agtacctgct gttccgcacc
     6121 gggcccatga ccatgccgcc gtcgcagctg ggcgcgttcg tgaagtccga cgcttcgcag
     6181 cctacgccca acctcgaata ccacgtgcag ccgatctcgc tcgacaagtt cggcgatccc
     6241 ccgcacccgt ttcccgcctt caccgccagc gtgtgcaacc tgcgcccgac ctcgcgcggc
     6301 cacgtgcgca tcacctcgcc cgacccgcgc gcctacccgg cgatcacttg caattacctt
     6361 tccacgaacg aggaccggaa gactgccgcc gcggcgatcc ggctcacgcg ccgcatcgtg
     6421 accggcacgc gggcgctgaa gcggtatgag ccggaggaat tcatgccggg gccggcgttc
     6481 cagtcggagg aggagctggt ccgggccgcg ggcgacatcg gcactacgat cttccatccc
     6541 gtcggcacct gcaagatggg gcgcgagagc gacccgtcgg cggtggtgga cccgaggctc
     6601 cgggtacgcg gcgtcgagcg gcttcgcgtc atagatgctt ccatcatgcc gaccatcaca
     6661 tccggcaaca ccaacacgcc cacggtgatg atcgcggaac gcggcagcgc gctcgtgcgc
     6721 ggtaaagatg gacgggattg agggcgcctg acgatgaggg ggagggaaag atgaataagg
     6781 cgaaaggcga aaggcgaaag gcgaaaggga cgatgccaag atccggtttt tacactttcg
     6841 actttcgact tccgactttc gccttgggga tagggctggc ggcgggcatg ctggcgctat
     6901 tcagcgccac ggccgccgca caggcctatc cccagcgccc gatccggctg ctggtgccga
     6961 acccgcccgg cggcgcgacc gacaatcttg cgcgcgtcgt cgcgcccaag ctcaccgagc
     7021 tgctggggca gaccgtggtg gtggacaacc gtccgggcgc ggccggcaat ctcgcggccg
     7081 aggcggcggc gagggcgacg cccgacggct acacgctgat gctcgctgcc gacgggcaga
     7141 tcgtgctcag cccacatctc taccggaagc tgccgttcga tacgctcaag gacctgacgc
     7201 cggtcgcgag cctcgtttcc acgcaaatgc tgctggcggt ccatccggcg ctgccggtga
     7261 agacggtccc ggaattcgtg gagtacgcgc gccgcgcgag tctgccgctc gcctacggct
     7321 cgatcggcaa cggcagccag catcatctcg ccatggaaat gctgaagaga cgcgcagaca
     7381 tcaacctgct gcacgtcccg tacaaaggag ggggcccggc tctggtgggc ctgttcggcg
     7441 gcgaggtgtc cgtgatgttc ggcggcagct ccgccgggcc tcacgtgcgc tcgggcaagc
     7501 tgcgcgcgct cgcgctcgcg ggcaagcgca atcccgcata tccgggcttg ccagcgctct
     7561 ccgacaccta tcccggtctc gaagtaaccc cctggctcgg gttgttcgcc ccggtggggc
     7621 tacctgcgac ggcgctcaac cggctgcgta ccgagaccgg ccgtatgctt gccgatcccg
     7681 actcgcgcgc gcgcgtgggc aacatcgcgc tcgacccttt cccgacgacg ccgcaggaat
     7741 tctccgcgct gattcgcgcc gactacgcga aatacggaga gatcatccgg agcgtcggcg
     7801 tcaagataga ttaaggcgaa aggcgaaagt cggaacagca cctgctctaa aggctttcct
     7861 tcctccttcc gcctttcgac ttccgccttc gagggggcga gctacctcgc ccccgggacg
     7921 ttgcgggtct tgaggaactg ctcgagtgtt tcaccacgga agctggccac catttccggg
     7981 ttggagacgc ccttgacgcg ggcaagcttc tcgaggcaga aaaaactcgc cccatagcgg
     8041 atcaacgcgg cccagtcgag ctcgcgcacc atgcgccgct cctctgcgtc gagacccgcc
     8101 ttctcgagcg cggattccgc atcctgcttg aatagcccgc gcttctccgg cacggtcagg
     8161 ccgtgcagga agcggttcag ccgcagcgcc ctggcgctgc gctcgagcgt gaagaggtag
     8221 gtcccctcca gcgcttcgac gccttcgagt tgcggattca ccgggctttc ctccgcactg
     8281 cggcgtgatt catgaatcgt gattcgaaga tttgcttcgg tccaatcact attcaccatt
     8341 cacgggttta atcagtacgt gcgcccgacg cgcggacgac cggcccccag cgcttgacct
     8401 cggcggtcat gtaatccgtg aacgccttcg catcgagctg gttcggctcg atgccgtcgc
     8461 cgcgcaggcg ctcgcgcacg tcgggaagct ggaggatccg ccgcacctcg gaattcaggc
     8521 ggttgacgac gtcggccggc acgccggccg ggccggacag cgagaaccag acggttgcca
     8581 ccaggtcacg gtagccgagc tcgcggaagg tgggaacgcc ggaataatcc ggcagccgat
     8641 tggccgaact gagcgcgaga gcgcgcacac gaccggcccg aatctgcgcg ctggcggtgg
     8701 tgagcgtggt cgagatcgcg tgaatgtggc cggcgacgat gtcgacgacg gcgatggcag
     8761 cgcccttgta cggcacgtgc acgatgtcga tcccgctctt ctgcttgaac agctcggcga
     8821 ccagatggcc ctgggtgccg acgccgggcg agccgtaggt cacgttacgc ggccttgcct
     8881 tggcgaacgc gacgaacccc ttgaggtcct tcactggaag cgaggggtgc gccgcaaaca
     8941 cggcgggcgg cccgccgaag agcgcgatgt gggtgaaatc cttgaccgga tcgaacgggg
     9001 gcttggccgc gagcgccggc gcgatcgcgt gcgaagcgac gccggagacg accagcgtgt
     9061 agccgtcggg agcgctcttc gagacgagtt cggagccgag cagtccgccc gcgcccggcc
     9121 ggttctccac gatgaaattc tggccgaggc tttcggtgag cttggcggcg acgatgcgcc
     9181 cgagcgtatc cgcggtgccc cccggcgcga acggcgcgat gatccgtacc ggcttgacgg
     9241 gatactgctg cggccacgcc ggcgccactg ccaggaatgc gggcaacagg caaccagctg
     9301 ctatgcgaaa catgtcatta ccttcctcgg tttatcagtc acgttttatc agtcacgctt
     9361 ctcgagcgct cagtccactt tgacgcccga agccttgacc gcccttgcgt atttggcgat
     9421 gtcaatgcgg atctgcgcgg cgtactgctc cggcgtgttg cccaccgggt cgatgccgag
     9481 cgtggtgtag cggttcacga tgtcggggtg ttgcaccgcc tttacgatcg ccgaagcgag
     9541 ccggttgacg atggctttcg gcgttttcgc cggcgcgagc acgccgatcg cgggcggata
     9601 atcgtagtcc ttgatgcccg tgatctcggc gatggtcggc acctccggcg cttgcgcgtt
     9661 gcgcctgatc gtattgacgg cgagcagctt caggcgcccg gccttgacgt gtggggcgat
     9721 ggagggcagc gcggcaaaaa ggagcgacac ttcgccggcg agcagcgcgg gcaccgcctg
     9781 tcccgtgccc ttgtacggga cgtgcacgat gtcgaggccg agcggcgttt tgagcgcctc
     9841 catgctgaga tgatggacgc ttcccgtccc cccggagccg tagcggaact ggcccggctt
     9901 gctcttcacg agcttgatga gctcgggaag cgtgttcgcc gggaccgagg cgtgcgccac
     9961 caggaattgc cccgaaattc ccatcaggct gaccggggcg aaatcccgga gcgtatcgta
    10021 aggcagcttc gtgtaaagcg cggggttgat cgcggtctgc gcgacgtccg tgatcaacaa
    10081 cgtgtagccg tcagggttgg atttggcgac cagctcacag gcgccgatgc cgcccgcccc
    10141 ggggcggttg tccacgacga tctgctgccc gagcgtttcg atcatcctct gggaagcgat
    10201 gcgggtcatc acgtccggca gcccgccggc cgcgtacgga atcacgacac ggatcggtcg
    10261 ggtcgggaag tcctgcgcag ccgcgctccc taaccatgcc gccgtcgcaa acacacacaa
    10321 gaaacgcagc cgctggcgca ccatgtgagt cctcccctga ttggttgtat ccccgccgcg
    10381 gggttacact gcaagggcaa ttataagcga gcacggattg gaaggagttg cgatgagcgt
    10441 taagggcttg attgatgatc tcgtgagcgc caaccacatt ttgttcaagg agggcgtggt
    10501 ggacgggttc ggccacgtca gcgtgaggga cgggaggaac cccgagcgtt tcctgctggc
    10561 gaagagcatc gcgcccgcga ccgtgaaggc cagggacatc atggaattcg gcatggacgg
    10621 agcacccttg aatccgcggg gccgcaagcc ttatctcgag cgctacatcc acagcgagat
    10681 ctaccgcgca cggcccgacg tgacggccgt cgtccacagc cactcgccgg cgctcattcc
    10741 gtttggcatc acgggaacgt ccctgcaccc ggtcttccac atgagcggtt tcctcggttc
    10801 cgcgacgccg atcttcgaga tacgcgaagc aggtggtccc gcgacggaca tgctgattcg
    10861 cagccgcgag ctcggcgcgg cgctcgcgcg gaccctgggt ccggcgccgt ttgcattgat
    10921 gcgcggacac ggctcggtcg cggtcggggc cacgctcaag caggtcgtct atcgcgccat
    10981 ctacgcggag gtcaacgcgc gcctgcaatc cgaagccatg cggcttggga aaatcaattt
    11041 cctgaacgac gaagaagccg ccaacgcgag ccggaccaac gacggcgtca tcgaccggcc
    11101 gtgggagctc tggaagaaac gggcacgaga atgagggata aggcgcgggt aggagtgccg
    11161 aggaatgagt gtcgagggat aacactcgac acccgacact cgtctcttgc aactcatcac
    11221 gcctttcagc atgtggactg gaagcatttg gcgcgagagc ttgtttacca agtgctgccg
    11281 tcctcgttat catgaggcaa ggaatcggca atgagcagtc agagcatcgt cctggcctac
    11341 ctgcaatcct gcaccagcgg caggctggga gtgtcggatt gcggcccgat atggcagctt
    11401 ctcgtcatcg ccgtgctcct gctcattgcg atcg
//
LOCUS       DBNGWZ010000191         4178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1658052, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000191 DBNGWZ010000000
VERSION     DBNGWZ010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1658052"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="AC16CX_05600"
     CDS             complement(<1..1150)
                     /locus_tag="AC16CX_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012022.1"
                     /translation="MNAPSRPVPGSTGSTGKAARDGAGVIVDYLIREKVPYVFGLCGH
                     GNIGFIDALHARAGEIKTVSVHHESVAGFMADVYYRVSGQPTATFTSCGPGSANLPIS
                     LANSFFDSVPFFAVTGNVPTSQFNRGAFQETYRHYQADFPSTVRAYCKRVFQPTTAAQ
                     LPVAVRQAWKTMVTGRPGPVVLDVPFDMFNEPIPEETPRPEDWSANISCRCGADPEGV
                     SRAADMLMAAQRPVILVGQGVRYGRATGELRALAEKLRIPVAMSASGIGAMDSTHPLA
                     LGLVARNGPYQANHAARQADVLLALGVRFDDRTSSSWIPGFSFTIPPTKLIHVDIDPD
                     EIARNYPVALGLMADVRTFLRQLLAEIDARLARGQMPAERGAWLDAIAG"
     gene            complement(1274..2236)
                     /locus_tag="AC16CX_05605"
     CDS             complement(1274..2236)
                     /locus_tag="AC16CX_05605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012023.1"
                     /translation="MQTYVRSALSCALMGLALCSVAQDYPSKPVRVIDPFSPGGATDV
                     LARVIGQKLSERLGQSFLVENRAGGGGHIGADLVVKSPPNGYTLLVAGVPHAIGMTLY
                     RKLPYDMARDLAPITQLATFPSLIVVHPSLPVKSVKELLALAKARPGELNYGANPGSP
                     NHLAMELFNSMGKVKMVHIGYKGAGPVVIDLLAGQLHLASLGVPTVWPQVQAGKVRVI
                     AVTSTTRSPLLPAVPTVSESGLPGYDVSSWYGMYSAAGTPGTIVTKLNAEIAVVLKTP
                     DVTERLSSLGAQPAPTSPEELGRITRDEIKKWAKVVQESGARVD"
     gene            2258..3580
                     /locus_tag="AC16CX_05610"
     CDS             2258..3580
                     /locus_tag="AC16CX_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3012024.1"
                     /translation="MSVALFEAKNICFSPVELNRNSVMRPESVAQGKHTAVRLAESCL
                     ARIEARDRDVQAWAYIEPEQVLAQAHARDREAPRSALHGIPVGVKDIIDTCDMPTEYG
                     SAIYAGHRPKADAACVALLREAGAVIMGKTVTVELAAFHWARTRNPHDLAHTPGGSSS
                     GSAAAVADFMVPLALGTQTGGSTIRPAAYCGVVGYKPSFNLINRAGVKPLAESQDTVG
                     LFGRSVPDVALICSILSGCPAPDLDRKDVGPPRIGLCTMAWPTTLQPAMAKAMEDSTA
                     RLARAGARISECALPQPFEASFLAQRKMNDYEAWRALSHERLLHAHQLSATLSARLKE
                     AALCTFDVYREAQAVISRCRALLDESFAKYDVLLTPSVPAEAPEGLTNTGDSTFIRIW
                     TAFHTPTITLPVFRGEKGLPMGLQVIGPIGEDNRTLVCAEWIFRTLTA"
     gene            complement(3769..>4178)
                     /locus_tag="AC16CX_05615"
     CDS             complement(3769..>4178)
                     /locus_tag="AC16CX_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3012025.1"
                     /translation="QMVTELLSGQVQLASIGLPPAMPYVKSGRLRVIAVTGMTRSALL
                     PDAPTVNESGLPGFDVTSWYGVFGPAALPKDIVARLNADVAAILGAPDLKERLAGLGA
                     EPGSMPQEEFARYVREEITKWAKVVKDSGATVD"
BASE COUNT          761 a         1354 c         1345 g          718 t
ORIGIN      
        1 agcccgcgat cgcgtcgagc cacgcgccgc gctccgcggg catctgcccg cgagccaggc
       61 gcgcatcgat ctcggcgagc agctgcctga ggaaggtgcg cacgtccgcc atcaggccca
      121 gcgcgaccgg atagttgcgc gcgatctcgt ccggatcgat atcgacgtgg atcagtttcg
      181 tcggcgggat ggtgaacgag aagcccggta tccaggaact cgacgtgcga tcgtcgaatc
      241 gcacgccgag cgcgagcagc acatcggcct gccgcgcagc gtggttcgcc tggtacggcc
      301 cgttgcgcgc caccaggccg agcgccagcg gatgcgtgct gtccatggcg ccgatgccgc
      361 tcgcggacat ggcgaccggg atccggagct tctcagccag cgcacgcagt tccccggtcg
      421 cgcggccgta gcgcacgccc tgcccgacga ggatgaccgg gcgttgcgcc gccatcagca
      481 tgtcggccgc ccgggaaacg ccttccggat cggcgccgca gcggcacgaa atattggcgc
      541 tccaatcctc ggggcgcggc gtttcctccg ggatcggctc gttgaacatg tcgaacggca
      601 catcgagcac gaccggaccg ggtcgcccgg tcaccatggt cttccacgcc tggcgcaccg
      661 cgaccggcag ttgcgccgcg gtcgtgggct ggaacacgcg cttgcagtag gcgcgcaccg
      721 tcgagggaaa atccgcttga tagtgcctgt aggtttcctg gaacgcgccg cggttgaact
      781 ggctggtcgg aacgttgccc gttaccgcga agaaaggcac ggagtcgaag aacgagttgg
      841 cgagactgat cggcaaattc gccgagccgg gcccgcagga ggtaaacgtc gccgtcggct
      901 ggcccgacac gcggtaatac acgtcggcca tgaatcccgc gacactttcg tgatgcacgg
      961 aaaccgtctt gatttcgcct gcgcgcgcat gcagcgcgtc gatgaacccg atgttgccgt
     1021 ggccgcacag tccgaagaca tagggcactt tctcgcggat caggtaatcg acgatgacgc
     1081 ccgcgccgtc gcgggcagcc tttccggtcg aacccgttga acccggcaca ggcctgctcg
     1141 gtgcgttcat gggaattctc ctgatgatta agtgactcgc ggatcgtgtc gagctacaac
     1201 tttccagccg gcgagagccg ggtgcacgaa ccgcgagtca cgagccgcga gtcacgccgt
     1261 tccagcgcga cggtcagtct accctcgcgc cggattcctg gaccactttg gcccacttct
     1321 tgatttcgtc gcgggtaata cgtccaagct cttccgggct cgtcggcgcg ggctgagccc
     1381 ccaggctgga gagccgctcc gtcacatccg gtgttttcag caccactgca atctccgcat
     1441 tgagtttcgt aacaatggtg ccgggagtgc ccgccgcgga gtacatgccg taccaggaac
     1501 tcacatcgta acccggcagg ccggattcgc tgaccgtcgg cacagcgggc agcagcgggg
     1561 agcgggtggt gctcgtcacg gcgatcacgc gtaccttgcc cgcctgcacc tggggccaga
     1621 ccgtcgggac accgagcgat gcgagatgaa gctggcccgc cagaagatcg atgacgaccg
     1681 gacccgcgcc cttgtatcca atgtggacca ttttcacctt gcccattgaa ttgaacagct
     1741 ccatagccag gtggttcggc gatccgggat tggcgccgta gttgagctcc ccgggacggg
     1801 ccttcgcgag cgcgaggagc tccttcacgg actttacggg gagcgacgga tggaccacga
     1861 tgaggctggg gaacgttgca agctgagtga tgggcgccag gtctctcgcc atatcgtacg
     1921 gcagcttgcg gtagagcgtc atgccgatgg cgtggggaac gccggcgacc agcagcgtat
     1981 agccattcgg tggcgacttg actaccaaat cggcgccgat gtggccgccg ccgccggccc
     2041 ggttctccac caggaacgac tggccgagcc gctcggaaag cttttgaccg atgacccggg
     2101 cgagcacgtc tgtcgctccg ccgggagaaa acggatcgat cacgcgcacc ggtttgctcg
     2161 ggtaatcctg ggcaacggag cacaacgcga ggcccatcaa agcgcaggac aaagctgaac
     2221 gaacgtacgt ttgcatgatc tttccttggc gaacggagtg agcgttgcgc tgttcgaggc
     2281 gaagaatata tgcttcagcc cggtggaact caaccggaat tccgtaatgc ggcccgagtc
     2341 agtagcgcaa ggaaagcata cggctgtccg gctcgccgaa tcatgcctgg cgcggatcga
     2401 agcgcgggac agggacgttc aggcttgggc gtatatcgag ccggagcaag tgttggcgca
     2461 ggcccacgcg cgggaccggg aagcgccacg cagcgcactt cacggtatcc cggtcggggt
     2521 caaggacatc atcgacacct gcgacatgcc gaccgagtac ggctccgcca tctacgcagg
     2581 ccatcgcccc aaggctgacg cagcgtgcgt cgcgctgctg cgtgaagcgg gcgccgtcat
     2641 catggggaag accgttaccg tggagcttgc cgcattccat tgggcgcgca ctcgcaatcc
     2701 gcatgacctc gcgcacacgc cgggtggttc atcgagcggc tctgcggcgg cggtcgcgga
     2761 cttcatggtg ccgctggcgc tgggcacgca gacgggcggc tcgacgatcc gccccgctgc
     2821 ctactgtggt gtcgtcggat acaagccgag cttcaacctg atcaaccggg cgggcgtcaa
     2881 gccgcttgcg gagtcgcagg acacggtggg gcttttcggc cgcagcgttc ccgatgtggc
     2941 gctgatatgc tcgattctat ccggctgccc ggcgccggac ctcgatcgca aagacgttgg
     3001 cccgccacgg atcggactgt gcaccatggc gtggccgacg acgttgcaac cggccatggc
     3061 gaaagccatg gaggacagta ctgcccggct ggcgcgcgcg ggcgctcgaa tcagcgaatg
     3121 cgcactgccg cagccgttcg aggcgtcgtt cctcgcgcaa agaaagatga acgattacga
     3181 agcctggcgc gccttgagcc acgagcgctt gttgcacgcg catcaattga gcgcgacctt
     3241 atcggcccga ttgaaggagg ccgcactgtg caccttcgat gtctatcgcg aggcgcaagc
     3301 cgttatttcc agatgccgcg ccctcctcga tgaaagcttc gcgaaatacg acgtgttgct
     3361 cacgcccagt gtgccggctg aagcaccgga agggctcacg aacaccggcg attcaacctt
     3421 tatccgcatc tggaccgcct ttcacacccc gaccatcact ctgccggtgt tccggggtga
     3481 gaaaggtctg ccaatgggat tgcaggtgat cgggccgatc ggcgaagaca accgcacgct
     3541 cgtctgcgcc gagtggatct ttcggacgtt gaccgcttaa gtcggatggt gggaaagcgg
     3601 acctagtcgc tgccgccgtt acctcacccc gagccgaatt ttacgcgcct cgcgcgtggg
     3661 aaataagaaa gcgtgataag tgataagggg ttcccgcatg tgtgggagtc agctcctatc
     3721 acctatcacc tatcacctat cacccattac gcctttccgg cgcgacggtc agtcaaccgt
     3781 agcgccggag tctttgacca ccttcgccca cttcgtgatc tcctcgcgca catagcgggc
     3841 gaactcctcc tgcggcatcg accccggctc cgcccccagc ccggcaagac gctccttgag
     3901 atccggcgcg cccaggatcg ccgcgacatc ggcgttcagc ctagcgacga tatctttcgg
     3961 cagcgcggcc gggccgaaca cgccgtacca cgacgtgacg tcgaaaccgg gcagtccgct
     4021 ctcgttgacc gtcggcgcgt cgggcaacag ggctgaacgc gtcatgccgg tcacggcaat
     4081 gacgcggagc ctcccggact tgacgtacgg catcgccggc ggcagcccga tcgaggcgag
     4141 ctgcacctgt ccggacagca gctcggtgac catctgcc
//
LOCUS       DBNGWZ010000192         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3293586, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000192 DBNGWZ010000000
VERSION     DBNGWZ010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3293586"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..780)
                     /locus_tag="AC16CX_05620"
     CDS             complement(70..780)
                     /locus_tag="AC16CX_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR3012026.1"
                     /translation="MKTTPAFGRWAALGLVLLLPAADRAVSHLLGIDRLLPDLSSEGG
                     LLLAWAIGHCDTRDGPVVMLAREALETGNVNRVLPWVRPEDEHEIRHAFDHALSVRKL
                     GPRARDLADEHFYEMVVRVHRSAEGAPYTGLKPAGLDLGPAVPAADKALRDGSVEAVV
                     KLLTDAVRAGVHLHFHAAMSRKDFAVDDIAAGRRYVEAYVPYVHYVERLWQAATGTVH
                     GHHPEHAKPAHEHAAHAH"
     gene            complement(928..1131)
                     /locus_tag="AC16CX_05625"
     CDS             complement(928..1131)
                     /locus_tag="AC16CX_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIR3012027.1"
                     /translation="MPWSDDRYPVSMKNLPPLVRHKAIEIANALLEEGYDEGRCIRIA
                     ISRAKRWESAHAGRSMSARRLNG"
     gene            complement(1115..1312)
                     /locus_tag="AC16CX_05630"
     CDS             complement(1115..1312)
                     /locus_tag="AC16CX_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012028.1"
                     /translation="MLRKRKLRRVMGALLVLAGALLMWLAPESLSGAVLLAAGIALEA
                     IGLGLEHRAGQSKDGPDALER"
     gene            1428..>1689
                     /locus_tag="AC16CX_05635"
     CDS             1428..>1689
                     /locus_tag="AC16CX_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3012029.1"
                     /translation="MKPFNAAAAAPDAYFEHDADIGVVGRGATLEEAFEAAARATFAI
                     MTDITAVQPSESVTFEFEEADRELALVRWLNLLLGLAREGGLV"
BASE COUNT          268 a          542 c          598 g          281 t
ORIGIN      
        1 ccaggacggc tgcctgtgtt ccgtggggcg aggcagaacc gtcggtcatg gagcgggccg
       61 ccgcgcctct cagtgggcgt gagcggcgtg ttcgtgcgcc ggcttggcgt gctccgggtg
      121 gtggccgtgc acggtgcccg ttgcggcctg ccacaagcgc tcgacgtagt ggacgtaggg
      181 cacgtaggct tccacatagc ggcgtccggc ggcgatgtca tccacagcga agtccttgcg
      241 gctcatcgcg gcgtggaaat gcaggtgcac gccggctcgc acggcgtcgg tgagcagctt
      301 caccacggcc tcgaccgagc cgtcccgcag cgccttgtcc gcagccggca cggcggggcc
      361 gagatcgagg cctgcgggct tgaggccggt gtagggggcg ccttccgcag agcgatgcac
      421 gcggaccacc atttcgtaaa agtgctcgtc cgccagatcc cgcgcgcgcg ggccgagctt
      481 gcgcacggaa agcgcatgat cgaaagcgtg ccggatctcg tgctcgtcct ccgggcgcac
      541 ccacggcagg acacggttca cattgcccgt ctcgagcgcc tcgcgcgcga gcatgacaac
      601 cggaccgtcg cgagtgtcgc aatggccgat ggcccaagcc agcagcaagc cgccttcgct
      661 ggacaggtcg ggcaacagcc gatcgatacc gagcaggtgc gaaacagcgc ggtcggcggc
      721 aggcaggagg agcaccaggc cgagcgcggc ccagcgtccg aaagcgggtg tcgtcttcat
      781 cgtgaaaccc tctgataagt tcgtttgcgc attgaagaat tctcgatatt ctggtgacgt
      841 gtgtcttgac ctggatcaac cggggtggag gcgcaacccg acgttttgag atcgcgtgcc
      901 gacaaagctg ggacgggctg cggcgcgcta gccgttcagg cggcgcgcgc tcatcgagcg
      961 ccccgcatgc gcagattccc aacgcttggc gcgcgagatc gcgatgcgga tgcagcggcc
     1021 ctcgtcgtac ccttcttcca gcagggcgtt cgcgatctcg atcgccttgt gccgcacgag
     1081 cggcggcaag ttcttcatcg agaccgggta gcggtcatcg ctccagggca tcggggccgt
     1141 cctttgattg gccggcgcgg tgctcgagtc cgagcccgat cgcctcgagc gcgatgccgg
     1201 ctgcgagcag cacagcgccg gagagcgatt cgggcgcaag ccacatgagc agcgcgcccg
     1261 cgagcacgag cagcgcgccc atgacgcgcc ggagcttgcg tttcctgagc atggttgctg
     1321 cgacctcgag cgtgtgccgg gactggaacc cagttaagcg cgattgcgcc cggagcggtt
     1381 tgacctaggt caacccggtg acgctttgac tggtgcagca tgccgccatg aagccgttca
     1441 acgccgccgc ggcggcgccc gacgcctatt tcgagcacga tgccgacatc ggcgtcgtgg
     1501 gacgcggcgc aacgctggaa gaggcgttcg aggcggcggc gcgcgccacg ttcgcgatca
     1561 tgactgatat caccgcggtg cagccctcgg agtcggtgac gttcgaattc gaggaggccg
     1621 accgcgagct tgcgctggtg cgctggttga accttctcct cgggctcgcg cgtgaaggcg
     1681 gtttggtct
//
LOCUS       DBNGWZ010000193         1984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2538875, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000193 DBNGWZ010000000
VERSION     DBNGWZ010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2538875"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC16CX_05640"
     CDS             complement(<1..678)
                     /locus_tag="AC16CX_05640"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3012030.1"
                     /translation="MSANPKFLSATAHVDEAAVKPLPNSRKVYVTGSRSEIRVPMREI
                     LQSDTPASFGSEKNPPIFVYDTSGPYTDPSTKIDIRRGLEPLRLAWILERNDTEELAG
                     PTSRYGRQRLADPKLAQLRFNLVRKPRRAVSGRNVTQMHYARRGIVTPEMEFIAIREN
                     QRMDALSELLRRQHAGESFGAAIQKTITPEFVRDEVARGRAIIPANINHPETEPMVIG
                     RNFLVKIN"
     regulatory      complement(716..817)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            886..1551
                     /locus_tag="AC16CX_05645"
     CDS             886..1551
                     /locus_tag="AC16CX_05645"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase
                     family protein"
                     /protein_id="HIR3012031.1"
                     /translation="MNPQLDIERARFNMVEQQIRTWEVLDQQVLDLLFRVHREEYVPP
                     QYRALAFADMEIPLGHGEKMLAPKLEARMLQELALRPTDKILEVGTGSGYMTALLASL
                     GGHVCSVDIIAEFTQTAGARLAANGIANVTLETGDAARGWDRHAPYDAIVLTGSVPVL
                     SQAFPAALQPGGRLIAVVGEPPVMEAQLITCVAAGVFNTTGLFETCIAPLRNAPQPER
                     FVF"
     gene            1681..>1984
                     /locus_tag="AC16CX_05650"
     CDS             1681..>1984
                     /locus_tag="AC16CX_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HIR3012032.1"
                     /translation="MIRSLRSFSFLILLAAATAAHGADLLEVYRLAQGSDAQYAAARA
                     AWSAGQEKLPQGLAGLLPSASVSASTQYNDRELRFRNPANPPSNAQFNSNSAALSVS"
BASE COUNT          340 a          636 c          611 g          397 t
ORIGIN      
        1 gttgatcttc accaggaagt tgcggccgat caccatcggc tcggtctcgg gatgattgat
       61 gttggcgggg atgatggcgc gcccgcgcgc gacctcgtcg cgcacgaact cgggggtgat
      121 cgttttctgg atcgccgcgc cgaacgactc tccggcgtgc tgccggcgca gcagctccga
      181 gagcgcatcc atgcgctggt tctcgcggat cgcgatgaat tccatctccg gtgtgacgat
      241 gccgcggcgc gcgtaatgca tctgggtcac gttccgtccg gaaaccgcgc gtctcggttt
      301 ccggaccaga ttgaaacgca attgagccag tttcggatcg gcgaggcgct ggcgtccata
      361 acgcgaggtg gggccggcca actcctccgt atcatttcgc tccaggatcc acgccagccg
      421 gagcggctca aggcccctcc gaatatcgat cttcgtgctc gggtccgtat acggacccga
      481 ggtgtcgtaa acgaagatcg gcgggttttt ctcgctaccg aaagaggccg gcgtgtccga
      541 ttgcaggatt tcgcgcatcg gcacgcggat ctcggaacgc gagccagtga cgtagacctt
      601 gcgcgagttc ggcagcggct tgaccgctgc ctcgtcaacg tgggcggttg cactcaggaa
      661 tttgggattg gcgctcatgg tcgtctctcc ggtaagcgcc gtgcgggagg tggagggcta
      721 tcccgccacg cttccctacg acggcattac ccacatcagg ttcgaagggt ctttctcacc
      781 cagctgccgg cggcaaccag gacccctaac gtgtcctttt aacctaatcc gattgtgcga
      841 taattgcaag ccccgtaatg tattttcccg ttttccgccg cttctatgaa cccgcaactg
      901 gatatcgagc gcgcgcgttt taacatggtc gagcagcaga tccggacctg ggaagtgctg
      961 gatcagcagg tgctcgacct gctgttccgc gtgcaccgcg aggagtacgt gccgccgcag
     1021 tatcgcgcgc tcgcattcgc cgacatggaa atcccgctcg gacacggcga gaagatgctc
     1081 gcccccaagc tcgaagcgcg catgctgcag gaacttgccc tgcgccccac tgacaaaatc
     1141 cttgaggtcg gcaccggcag cggttacatg acggcgctgc tcgcaagtct cggcggccac
     1201 gtctgcagcg tggacatcat cgccgaattc acgcaaaccg cgggcgccag gcttgccgcc
     1261 aacggcatcg ccaacgtcac cctggaaacc ggcgacgccg cgcgcggctg ggaccggcac
     1321 gctccctacg acgcgatcgt gctgaccggc tcggtgccgg tgctgtcgca agcgttcccg
     1381 gcggctctcc agccgggcgg acggctgatc gcggtcgtgg gcgagccccc ggtcatggag
     1441 gcgcagctca tcacctgcgt tgcagcgggt gttttcaaca cgacgggatt attcgagacc
     1501 tgcatcgccc cgctccgaaa cgctcctcaa cccgagaggt ttgtgttctg aggaaatggg
     1561 aaattggaaa taggaaatgg taacagcagt acttttgata atgcgcggag cgcgggttgc
     1621 ggatttccat ttcctgttta ccgttcaccg ttcaccgttc accgttcact ggcagacgtc
     1681 atgattcgct cgctcagatc gttttcgttc ctaatcctgc tggccgcggc aacggcggct
     1741 catggtgccg atctgctgga ggtttatcgc ctggcgcagg gctcggacgc gcagtacgct
     1801 gcggcgcgcg cggcgtggag cgcgggccag gagaagctgc cgcagggcct cgccggcctg
     1861 ctgccctccg cttccgtttc cgcttccacc cagtacaacg accgcgagtt gcgattccgc
     1921 aatcctgcca atccgcccag caacgcgcag ttcaactcca actccgccgc gctgtcggtg
     1981 tcgc
//
LOCUS       DBNGWZ010000194         2139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_524377, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000194 DBNGWZ010000000
VERSION     DBNGWZ010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_524377"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="AC16CX_05655"
     CDS             complement(<1..1679)
                     /locus_tag="AC16CX_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3012033.1"
                     /translation="MNTLPGLLAAACALWGAQTGYWLIAAAAAVALEAPRFVTLRWNV
                     EQAHFNRLSDFCSALIVAAGVYLYFTYGNPRALMLLFQWMPVLMLPLALAQAWGNLRE
                     VDIAAFVWTLRKSTAAERFALNLGYPCLAVWVAAAAAANARGPAFYIGLTALVAWALW
                     AARPRRYPVVLWAALLAAAVGAGYGIQHGLYRFQAWMEEVIPEWIGASGSRTDPYRSR
                     TDLGTIGELKQDDAIVLRVRADGTMKTPLLLHRATYNSYFGRTWSARNAPLVARPPEA
                     GTRWVLDRDAAPGARVTVFDYSPRGNPVLSLPRGTVELRGLEALSLLRNGLGTVQAEL
                     PPGYFTYIAAVNPEAALEDGPNEEDLRIPSGERPLFGEIAGRVGLAGLPAEQAIEGVR
                     RYFADGFGYSLYQEKSLGSRSALADFLLRTRAGHCEYFATATVLMLRAAGVPARYATG
                     FSAQEYSRLENAWIVRVRHAHAWAKAYVDGRWVYVDTTPAIWARAEEQAASSWWSPVA
                     DLWSWLRFRLSQLGAGAKDEERTTAIVIVVALLIGLWFGWRLYKQRQLMMFG"
     gene            complement(1676..>2139)
                     /locus_tag="AC16CX_05660"
     CDS             complement(1676..>2139)
                     /locus_tag="AC16CX_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3012034.1"
                     /translation="ERHALVLDTFMAARDEHSFEEAVSIAASFAYTIDTRECLLDLMF
                     IGAEAYTYTAGRGQMQTGSLLEILAGVQPCRGKPFSVLANAVMTRRQALTGCICVLLG
                     WDEARREFVRRLHSHGVAALVIAVSRDPVADPPAWLRVVESDKIQEGLAQL"
BASE COUNT          359 a          765 c          726 g          289 t
ORIGIN      
        1 ccgaacatca tcaactgccg ctgcttgtaa agccgccagc cgaaccacag cccgatcagc
       61 agcgcgacga cgatgacgat tgccgttgtc cgctcttcgt ccttcgcgcc cgcgccgagc
      121 tgcgacagcc ggaagcgcag ccacgaccac aggtccgcaa ccggcgacca ccacgaggaa
      181 gccgcttgct cttcagcccg cgcccagatg gcgggcgtcg tatccacgta cacccagcgg
      241 ccgtcgacgt aagccttcgc ccaggcatgg gcgtggcgca cccgcacgat ccaggcgttt
      301 tcgagccggc tgtactcctg cgccgagaat cccgtggcgt agcgagcagg cacgcccgcg
      361 gcgcgcagca tcagcaccgt cgcggtggcg aagtactcgc agtggcccgc gcgcgtcctc
      421 aacaggaaat cggcgagcgc ggaacggctg ccgaggctct tttcctggta gagggaatag
      481 ccgaagccgt cggcgaagta acgcctgacc ccctcgattg cttgctccgc aggcaagccg
      541 gccaggccga cgcgccccgc gatttcgccg aacagcggcc gctcgccgga tggaatccgg
      601 aggtcttcct cgttggggcc atcctcgagc gccgcctcgg gatttaccgc cgcgatgtag
      661 gtgaaataac ccggcggcaa ttccgcctgc accgtgccga ggccgttgcg cagcaggctc
      721 aacgcctcga gcccgcgcag ctccacagtg ccgcgcggca ggctcagcac cgggttgccg
      781 cgcggcgagt agtcgaacac ggtcacgcgc gcgcccggag cggcgtcgcg gtcgagcacc
      841 cagcgcgtgc cggcttcggg cggccgcgcc acgagcggcg cgttgcgcgc gctccaggtc
      901 cggccgaagt agctgttgta ggtcgcgcgg tgcaggagca ggggcgtctt catcgttcca
      961 tccgcgcgca cgcgcagcac gatggcgtcg tcctgcttca actccccgat ggtgccgaga
     1021 tcggtgcggc tgcggtaggg atcggtgcgc gagccgctgg cgccgatcca ttccgggatg
     1081 acttcctcca tccaggcctg gaatcggtac aggccgtgct ggatgccgta gccggcgccg
     1141 accgccgcag cgagcagcgc ggcccacagc acgacgggat aacgccgggg ccttgccgcc
     1201 cacagcgccc aggccacgag cgccgtcagg ccgatgtaga acgccgggcc gcgcgcattg
     1261 gccgccgccg cggcggcgac ccacaccgcg agacagggat agccgagatt gagcgcgaag
     1321 cgctcggcgg ccgtgctctt gcgcagggtc cagacgaagg cggcgatgtc cacctcgcgc
     1381 aagttgcccc aggcctgggc gagcgcgagc ggcagcatca gcaccggcat ccactggaac
     1441 agcagcatca gcgcccgcgg attgccgtag gtgaaataaa gatagacgcc cgccgccacg
     1501 atcagcgcgc tgcagaaatc cgacaggcgg ttgaaatgcg cctgctcgac gttccagcgc
     1561 agcgtcacga agcgcggcgc ctcgagcgcg acggccgccg ccgccgcgat gagccagtag
     1621 ccggtctgcg cgccccacag cgcgcaggcg gcggcgagca gcccggggag cgtgttcata
     1681 actgcgcgag cccctcctgg atcttgtccg actccaccac ccgcagccac gccggcggat
     1741 cggccacggg gtcacggctc accgcgatca cgagcgcggc gacgccgtga gagtgcaggc
     1801 gccggacgaa ctccctgcgc gcctcgtccc atccgagcag cacgcagatg cagccggtga
     1861 gggcctggcg ccgcgtcatc acggcgttcg ccagcactga gaacggctta ccgcggcacg
     1921 gctgcacgcc ggcgaggatc tcgagcaggc ttccggtctg catctgcccg cggcccgccg
     1981 tataggtata ggcttcggcg ccgatgaaca ttagatcgag caggcactcg cgggtgtcga
     2041 tcgtgtaggc gaaggaggcc gcgatggaca cggcttcctc gaagctgtgc tcgtcgcgcg
     2101 cggccatgaa ggtgtcgagc accagcgcgt gccgctcga
//
LOCUS       DBNGWZ010000195         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1027625, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000195 DBNGWZ010000000
VERSION     DBNGWZ010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1027625"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16CX_05665"
     CDS             complement(<1..187)
                     /locus_tag="AC16CX_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3012035.1"
                     /translation="MRWKSDPTVWYCVRMKRCPYCAEEIQDDAIKCRYCGSFLNRRIW
                     RTARLYRSVRERKLAGIC"
     gene            215..658
                     /locus_tag="AC16CX_05670"
     CDS             215..658
                     /locus_tag="AC16CX_05670"
                     /inference="COORDINATES: protein motif:HMM:NF028108.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HIR3012036.1"
                     /translation="MTSPLRSPRETLGGYVILPRLIDKVRLHARGKLPPEYHPQLLAP
                     EPALDGRFLAFTGLNAEALRAAILASRDDAAALSWVERYGKPRTDAEKMAWAGAIDAY
                     RPDPERAARRAQNYPTVAARFDVGGMSVFDLIDLDEGRIAAPTSR"
     gene            682..1245
                     /gene="sigZ"
                     /locus_tag="AC16CX_05675"
     CDS             682..1245
                     /gene="sigZ"
                     /locus_tag="AC16CX_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HIR3012037.1"
                     /translation="MSTDAIWTAFADPLRRFIRTRVANDAEAEDILQEVFTRIHLKLA
                     QLRDTERLAAWIYRIARNAVIDHLRARAKAPAAVEVAEDLPEPAEAPALDAEVASCLR
                     PLIERLPDPYREALVMTELDGKTQRELARAAGLSLSGAKSRVQRGREQLKALLLACCH
                     VEFDRRGGVIAYEPRPGACRFCDPSAP"
     gene            1333..1854
                     /locus_tag="AC16CX_05680"
     CDS             1333..1854
                     /locus_tag="AC16CX_05680"
                     /inference="COORDINATES: protein motif:HMM:NF022321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HIR3012038.1"
                     /translation="MNLTSNQSTPRRITIEFLYLDLARCTRCRDTDRNLEAALADAAR
                     ILRAHGVDVEVRKIHVQSEEQARELTFVSSPTIRVNGRDIAFELKESRCEGCSCGCED
                     DGVTCRVWTYQGRDYDAAPKELIVDAVLKAASDSSSEPTQTVNRIADVPENLKRFFAA
                     KRREVSARSNACC"
BASE COUNT          350 a          723 c          655 g          302 t
ORIGIN      
        1 cgcagatgcc ggcgagcttg cgttcccgca ccgaccgata caaccgggcg gtcctccaga
       61 tccgccggtt caggaacgac ccgcagtagc ggcacttgat cgcgtcgtct tgaatctctt
      121 cggcgcaata ggggcagcgt ttcatgcgga cacagtacca aacggtggga tcggatttcc
      181 agcgcatcca gactgtccac cggtgcgaga gtcggtgaca tcgcccttgc gctcgccgcg
      241 cgagaccctc ggcggctacg tgatccttcc gcgcctcatc gacaaggtcc ggctgcacgc
      301 gcggggcaag ctcccgccgg aataccaccc ccaattgctc gccccggagc cggcgctcga
      361 cggccggttc ctcgcgttca cgggtctgaa tgccgaggcg ctgcgcgccg cgatccttgc
      421 ttcgcgcgac gacgcggccg ccctctcatg ggtggaacgc tatggcaagc cgcgaaccga
      481 cgcggagaag atggcgtggg ccggggcgat cgacgcctac cggcccgacc ccgaacgagc
      541 cgcccgccgg gcgcagaact atcccaccgt tgcggcgaga ttcgacgtcg gcgggatgag
      601 cgtgttcgac ctcatcgacc tcgacgaggg gcggatcgcc gccccgacca gccgatgacc
      661 gggcgcgccc ccaacgtcgc cttgtcgacg gacgcaatct ggacggcgtt cgccgatccg
      721 ctgcggcggt tcatccgcac gcgcgtggcg aatgacgccg aggccgagga catccttcag
      781 gaggtcttca ccaggatcca tctcaagctc gcgcaactgc gcgacaccga acgcctcgcg
      841 gcgtggatct accggatcgc caggaacgcc gtgatcgacc acctccgcgc ccgggccaag
      901 gcgccggcag ccgtcgaggt cgccgaggat ctgccggagc ccgccgaggc ccccgcgctc
      961 gacgccgaag tggcgagctg cctccggccg ctcatcgagc gcctgcccga cccctaccgc
     1021 gaagcgctgg tcatgaccga gctggacggc aagacgcagc gcgagctggc ccgcgcggcg
     1081 gggctgtcgc tgtccggcgc caagtcgcgc gtgcagcgcg gccgggaaca gctcaaggcc
     1141 ctgctgctgg cgtgctgcca cgtcgagttc gaccgccgcg gcggcgtcat cgcctacgag
     1201 ccccggcccg gcgcctgccg cttctgcgat ccgtccgcac cctgactgcg tccttttccc
     1261 cgaccctccg tctatacgag tgagcggccc cgcgcggccg aacacagaca ccctatgtta
     1321 aggaggatgg tgatgaatct gacatcgaat cagtcgacgc cccgccggat cacgatcgaa
     1381 tttctctacc ttgacctggc ccgctgcacg cgctgccggg acaccgaccg gaatctggag
     1441 gcggcacttg ccgacgccgc ccggatcctc agggcacacg gtgtcgacgt cgaggtccgc
     1501 aagatccacg tgcaatccga ggaacaggcg cgagagctga ccttcgtcag ctctcccacc
     1561 atccgcgtca acggccgcga tatcgccttc gagttgaagg agagccggtg cgagggctgc
     1621 agttgcgggt gtgaagatga cggcgtcacc tgccgcgtgt ggacctacca gggccgagac
     1681 tacgacgccg cgcccaagga gctgattgtc gacgccgtgc tcaaggccgc gtcggattca
     1741 tcgagcgagc cgacacagac cgtcaaccgg atagccgacg tgcccgagaa cctcaaacgg
     1801 ttcttcgccg ccaagcgccg tgaggtgtct gcccggtcga atgcctgttg ctgaagacgc
     1861 tcacgcgtcc ggcggccgct gattcgaccg cgcgtccttg acgtgccggt agagcgcaaa
     1921 tcccgtcgcg gcgatccccg tgacgatgag gccgaggctc gcattgacca gaaaccggtc
     1981 gtgttcggag agaccgtaga cggcgagcag aaacccgccc acgaagaaga
//
LOCUS       DBNGWZ010000196         4194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1279376, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000196 DBNGWZ010000000
VERSION     DBNGWZ010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1279376"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="AC16CX_05685"
     CDS             <1..642
                     /locus_tag="AC16CX_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3012039.1"
                     /translation="YDSLRDFAPVTQLTSGPAIVVVHPSLPARSIKELIQLARSRPGQ
                     LIYASAGLPSQLAVELFKVMTGTNMLHVPYKGAAPAMIDLIAGHVQVSFPTIIGALAH
                     VQAGRLRALATTGAKRAPAAPDLPTMVEAGVPGYEAANWFGTVVPAKTPPAVVSRLSQ
                     EIARVLRMPDVRDRLLFQGMEPVSNTPEDFSAYIRSEMAKWAKAVKASGAKPE"
     gene            complement(770..1531)
                     /locus_tag="AC16CX_05690"
     CDS             complement(770..1531)
                     /locus_tag="AC16CX_05690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3012040.1"
                     /translation="MTTIAKAIVRKIADLLGIRIAAANDPVLFFHSDRYLRHTARSLE
                     HLASLRIPVSRMTVLEVGAGIGDHSTYYMDRGCTITITEERADNLRILKRRYPDQNIQ
                     YLDMESPVAISGAPFDVVHCYGLLYHLKNPENALRFMSSLCRGKLILETCVSFGDVEA
                     INRVAEDQGNPTQASSGTGCRPTRAWVFSRLRQLFEHVYVPRTQPNDEEFPLDWTTSD
                     HPPGRLSRAIFIASRDVISNEMLLPSLISRQERHP"
     gene            complement(1644..1778)
                     /locus_tag="AC16CX_05695"
     CDS             complement(1644..1778)
                     /locus_tag="AC16CX_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012041.1"
                     /translation="MIAHMKDLGTLQVGAPGDYRFSNWWRERWASRIRARIRDTATVT
                     "
     gene            1867..3357
                     /locus_tag="AC16CX_05700"
     CDS             1867..3357
                     /locus_tag="AC16CX_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3012042.1"
                     /translation="MTAVADAAPVDLARQREVVAALQAFLPEASILFDGEDVKPYECD
                     GLTAYRQLPMVVALPETEEQVCRVLRTCHELRVPVVARGAGTGLSGGALPLGNGVLLS
                     LAKFMRIVEVDALARTARVQPGVRNLAISDAAAPLGLYYAPDPSSQLACTIGGNVAEN
                     SGGIHCLKYGLTVHNILRLRVATIEGEIVEIGAEALDAAGYDILALMTGSEGMLGVVI
                     EVTVRLLPTPEYAQVVMAAFDDIEKAGQAVANVIAAGIVPAGFEMMDQAATRAVENFA
                     RAGYRTDVAAVLLCESDGTREEVANEVRRIQEVMEKSGAVQTQVSRDEAQRLRFWAGR
                     KAAFPAAGAISPDYYCMDGTIPRKHLGAVLRRITELERKYGLRCANVFHAGDGNLHPL
                     ILYDANQPGELARTEAFGAEILELCLEVGGTITGEHGVGVEKINQMCVQFKAAELEAF
                     HGIKRAFDEHGLLNPGKAVPTLRRCAEFGRMHVHAGQEKFPDLPRF"
     gene            3543..>4194
                     /gene="glcE"
                     /locus_tag="AC16CX_05705"
     CDS             3543..>4194
                     /gene="glcE"
                     /locus_tag="AC16CX_05705"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR3012043.1"
                     /translation="MQDVVENLSRLIREAASQKRPLCIRGGGSKDFYGSAIHGYKLNT
                     VDYRGIVNYEPTELVITARAGTPLAEVESALREKGQMLAFEPPHFGESATLGGCVAAG
                     LSGPRRPHAGAVRDFVLGVRVLDGKGDDLRFGGQVMKNVAGYDVSRLMAGSLGTLGML
                     LEVSLKVLPLPAFETTLRLECGEAEAIVRMSQWAAQPLPITATAWHGGNLSVRLSGA"
BASE COUNT          776 a         1325 c         1352 g          741 t
ORIGIN      
        1 tacgacagcc tgcgcgattt cgcgccggtg acgcagctta cttccggtcc cgcgatcgtg
       61 gtcgtgcacc cttccctgcc ggcacgctcc atcaaggaat tgatccagct ggctcgatcc
      121 cgccccgggc agctcatcta cgcatccgcc gggctgccca gccagctcgc cgtcgagttg
      181 ttcaaggtca tgactggcac gaacatgctc cacgtgccgt acaagggcgc ggcgccggcc
      241 atgatcgatc tcatcgccgg ccacgtgcag gtgagcttcc ccacgatcat cggggcgctc
      301 gcgcacgtac aggccggcag gctgcgcgcg ctcgcgacga ccggggcgaa gcgtgctccc
      361 gcggcgcccg atctgccgac catggtggaa gccggcgtgc ccggctacga ggccgcaaac
      421 tggttcggca ccgtggtccc ggcgaagact ccgcccgccg tcgtctcgag gctgagccag
      481 gaaatcgccc gcgtgctgcg catgcccgac gtgcgagacc ggctgctctt ccagggcatg
      541 gagcccgtca gcaacacgcc cgaggacttc tccgcctata tcaggtcgga aatggcgaag
      601 tgggcaaaag cggtgaaagc ctccggagcg aagccggagt agctttaacg agaacaggat
      661 cagccaccgg cggagcgtgg cgcgccgatt gccggcggat ctgcgggctc gtagcagccg
      721 gcgtgatacg cgaagccgct cacctgcatc ggatggccag gccatttgtt catggatgcc
      781 gttcctgacg agaaattaac gagggaagaa gcatctcgtt gctaattaca tctcgtgaag
      841 cgataaagat tgctctactt aatcgaccgg gcgggtgatc cgaagtcgtc caatccaggg
      901 gaaactcctc gtcattgggt tgtgttctcg ggacatacac gtgctcgaaa agctgcctca
      961 aacgtgagaa tacccatgct cgggtcgggc gacatcctgt gcccgaagac gcctgcgtag
     1021 gattaccctg gtcttcggca acgcgattga ttgcctcaac gtcgccgaag gagacgcacg
     1081 tttcaagaat aagcttccct cggcagagag aactcataaa ccttaaagcg ttctccgggt
     1141 ttttcaggtg atacagcagc ccgtaacagt gaaccacatc aaacggcgcc ccgcttattg
     1201 ctaccgggct ctccatatca agatattgaa tgttctggtc agggtatcgg cgcttcagga
     1261 tgcggagatt gtccgcccgc tcctctgtaa ttgttatcgt gcagcccctg tccatgtaat
     1321 acgtcgaatg atcgccgata cctgcaccga cttcgagcac ggtcattctg ctcaccggga
     1381 tccggaggct cgcgaggtgc tctaaacttc gtgcggtatg gcgcaagtag cgatccgaat
     1441 gaaagaatag aaccggatca ttcgcggcgg cgatgcggat tccgagtaaa tcagcgattt
     1501 ttcgcacgat ggctttggcg attgtggtca taatcgaaag gacttccgcg ggtgtagagg
     1561 gccccgcttc aaataaggat aagtaaccgg caacggatac ggccgagcga acgtccgccc
     1621 cgcccgcatg acgggcgcgt gcttcaggtg acggttgccg tgtcgcgtat tcttgcgcgt
     1681 atccgcgagg cccaccgctc cctccaccag ttcgaaaacc ggtagtcgcc cggcgcgccg
     1741 acctggagcg tgccaaggtc cttcatgtgc gcgatcacac gcgcgattgc gttaaaactt
     1801 acgcgcgtgc cctttggcgg tcaagccgtg accgaaacga ttaccggttt gagatagaat
     1861 gccagcatga ctgccgtcgc tgacgccgct cccgtcgatc tcgcccgcca gcgcgaggtg
     1921 gttgccgcgc tgcaggcctt ccttcccgaa gcgtcaatcc tgttcgatgg cgaggacgtc
     1981 aagccctacg agtgcgacgg gctcaccgcc taccgccagc taccgatggt ggtcgcgctg
     2041 cccgaaaccg aggagcaggt ctgccgcgtt ctgaggacat gtcacgagct cagggtgccg
     2101 gtggtcgcgc gcggggcggg cacggggctc tcgggcggcg cgctgccgct cggcaatggc
     2161 gtgctgctct cgctcgcgaa gttcatgcgc atcgtcgagg tggacgcgct tgcgcgtact
     2221 gcgcgggtgc agccgggcgt gcgcaacctc gcgatctcgg acgcggccgc tccgctcggg
     2281 ctttattacg cgccggatcc ctcgagccag ctcgcgtgca ccatcggcgg caatgtcgcg
     2341 gaaaattcag gcggcatcca ctgcctgaag tacgggctca cggtgcacaa catcctgagg
     2401 ctacgcgttg ccaccatcga gggcgagatc gtggagattg gagccgaagc cctcgatgcc
     2461 gccggctacg atatcctcgc gttgatgacc ggctccgaag gcatgctggg cgtggtgatc
     2521 gaggtgacgg tgagactact gccgacgccc gaatacgcgc aggtggtgat ggccgcgttt
     2581 gacgacatcg agaaggccgg ccaggcggtg gcgaatgtca tcgccgccgg aatcgtcccc
     2641 gccggcttcg agatgatgga tcaagccgct acccgcgcgg tggaaaattt cgcgcgcgcg
     2701 ggctaccgca cggatgtcgc ggcggtcctg ctctgcgaat ccgacggcac gcgcgaggaa
     2761 gtggccaacg aggtacggcg catccaggaa gtgatggaga agagcggcgc ggtgcagacg
     2821 caggtctcgc gcgacgaggc ccagcggctg cgcttctggg ccgggcgcaa ggccgcgttt
     2881 cccgcggcgg gcgcgatctc gcccgactac tactgcatgg acggcacgat cccgagaaag
     2941 cacctgggcg cggtgctgcg ccggatcacg gaactggagc gcaagtacgg gttgcgttgc
     3001 gccaacgtgt tccacgcggg cgacggcaac ctgcatcctc tcatcctcta cgacgcgaac
     3061 cagccgggcg agcttgcccg cactgaagcc tttggggcgg agattctcga gctgtgcctc
     3121 gaggtcggcg gcaccatcac cggcgaacac ggcgttggcg tcgagaagat caaccagatg
     3181 tgcgtgcagt tcaaggcggc cgagctcgag gccttccacg gcatcaagcg cgcgttcgac
     3241 gagcacggcc tgctcaatcc cggcaaggcg gtgccgacac tccgccgctg cgccgagttc
     3301 gggcgcatgc acgtgcacgc gggccaggag aagtttcccg atttgccgcg cttctgaatc
     3361 cgtgatgggt gatgagggtg gagtgtcgag gaatgagtgt cgagggacgg gaaaagagtg
     3421 tcgaggactg agtgtcgagt gatgataggc aagaacgctg aatcagaagc tctttccctc
     3481 gacacgcgac actcgtcact cgccacccga ccctcgacac ccgacactcg tcactttcat
     3541 ccatgcaaga cgtcgtcgaa aacctgtctc gcctgatacg agaggcggcg tcgcaaaagc
     3601 gcccgctgtg catccgcggc ggcggcagca aggatttcta cggcagcgcg atacacggct
     3661 acaagctcaa caccgtcgat taccgcggta tcgtcaacta cgagccgacc gagctggtga
     3721 tcactgcgcg cgccggcaca ccgctcgccg aagtcgagtc cgcgctgcga gaaaaaggcc
     3781 agatgctcgc attcgagccg ccgcatttcg gcgaatcggc gacgctgggg ggctgcgtcg
     3841 ccgccggcct gtccggcccc cgccgccctc atgccggcgc ggtacgcgat ttcgtgctcg
     3901 gcgtgcgcgt gctcgacggc aagggcgacg atctgcgttt cggcggccag gtcatgaaga
     3961 acgtggcggg ctacgacgtc tcgcgcctca tggcgggctc gctcggcacg ctcggcatgc
     4021 tcctcgaagt atcgctcaag gtgctcccgc tgccggcctt cgagacgacg ctgcgtctcg
     4081 aatgcggcga ggccgaggcc atcgtgcgca tgagccagtg ggcggcgcaa ccgctgccga
     4141 tcaccgcaac ggcctggcac ggcggcaatc tcagcgtgcg gctgtccgga gcgc
//
LOCUS       DBNGWZ010000197         2893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1406412, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000197 DBNGWZ010000000
VERSION     DBNGWZ010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1406412"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..942)
                     /gene="modA"
                     /locus_tag="AC16CX_05710"
     CDS             complement(193..942)
                     /gene="modA"
                     /locus_tag="AC16CX_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3012044.1"
                     /translation="MKLSIAIACFLSVASLAEAAEIKVLSGSAVVPPMDVLIPQFERS
                     TGHKVMSDFDGAIGAMTKRIENGEAADVIIVSRPQIDFLEKLGKVIPGSAVEVGKLGV
                     GVFVRKGARKPDITSVEAFKRTLLAAKSIGYNDPAAGAPVSIYLISALERLGIAEQMK
                     PKTVVFKQRSERFAAVARGDVEIGFNQISEILAVPSVELVGPLPAAIQNYTLFTAAIV
                     ASSKQPDAAKAFVHFIASPTAATVLKAKGFE"
     gene            complement(1547..2023)
                     /locus_tag="AC16CX_05715"
     CDS             complement(1547..2023)
                     /locus_tag="AC16CX_05715"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HIR3012045.1"
                     /translation="MTRQQVEDFLYEEAALLDSWRLDDWLKLLTEDALYRVPSNDRPD
                     ADSKDTLFIIADDIRRIRARVTRLKDKSAHAEYPPSRTRRMISNVRIVSRNGASLGVE
                     ANFTVHRFRRNEGIREYVGHYRYELRVEGGQLKIAKREAVIDAMELGSLGSVSFIL"
     gene            complement(2159..>2893)
                     /locus_tag="AC16CX_05720"
     CDS             complement(2159..>2893)
                     /locus_tag="AC16CX_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3012046.1"
                     /translation="EVIQGVQDYDIRANWKLLAENSFDDYHLLSVHGSWLNYLKSSGV
                     EMKRPEKGHLLPAHGVGKELGNGHGCTDNVNFRGRPVARWIPIYGEAAKPEIERIRAE
                     LVARLGEERATRVADTNRNLLIFPNLFLNDGSSVTIRTFYPLAVDRMRVLAWALGPKE
                     ESPTARAVRLDSFLTFYGPGGFATPDDIEVLESVQSGLSTHREVPWSEITRGMAKEGE
                     QLNTDEPHLRAFWVQWDKLMRSERQG"
BASE COUNT          559 a          876 c          862 g          596 t
ORIGIN      
        1 gtagtcgtag ggcttgaagc gcgccatcgc cgcctcccac gtgctgaggg gcgaagcgcc
       61 cctcctcaca ccttagtacg acggcataac aacgagttcc ggagtttttc tacagattca
      121 acgttccatt aacccgcgcg ccgcttttgg cgcgtcggag ttgaatgggt tatacggctt
      181 cttgcaatca ctctactcga agcctttcgc cttcaagacc gtagccgccg tgggggaggc
      241 aatgaaatgg acaaatgcct ttgcggcgtc cggctgcttg ctactcgcaa cgattgccgc
      301 ggtaaacagc gtgtagttct gtatggctgc gggaagcggg cccacgagtt cgacgctcgg
      361 tacggccaag atttcgctga tctggttgaa gccgatttcg acatcgcccc tcgcaacggc
      421 tgcaaatcgc tcagagcgct gcttaaaaac gaccgtcttc ggcttcattt gttcggcgat
      481 gcccagccgc tccagcgcac taatgaggta gatgctgacc ggtgctccag cggccggatc
      541 gttgtagccg atggatttcg ccgccagcag tgtacgtttg aatgcctcga ccgaagtaat
      601 gtcgggcttc cgggcgccct tgcggacgaa gaccccgact ccgagtttcc cgacttcgac
      661 cgcactgccg ggaatgacct tgccaagctt ctccagaaaa tcgatctgcg gccgcgaaac
      721 aatgatgacg tcggcggctt cgccgttctc gatgcgcttc gtcatggcgc cgatcgcgcc
      781 gtcgaagtcc gacatcactt tgtgacccgt ggatcgttcg aactgcggga ttagcacatc
      841 catgggcggc actaccgcgg accctgaaag aaccttgatt tcggcggcct ccgccagcga
      901 ggcaactgag agaaaacagg caatcgcgat cgataacttc acggcgacaa atgcacagcg
      961 tcttattggc accatatcta cctcaagccg tataacgtgt cccgatcacc cgcgcgagca
     1021 agcgcccgct tgcgggcgtc ggcatgtgga tcgggtggtt agttggcatc acgtggaatt
     1081 ggaaatgggc gctacggact cttgcctttg ccaggggaaa tcgttacggc gtcaggaggt
     1141 gcttgatccc ccacgctcac aacccacact gttagaacta ccgcaggacc caaccaaata
     1201 cctgctgcca ccgcgacccg aatccatttc gcgcgcttcc gtagcaacca gtacgccaat
     1261 gcggccaccg gcagaaatgg cagtgaatag agaagaaggg cggtcatgtc cttgatcagt
     1321 gcccatgcca actaactata aatatccgac aatgatgccg tataacatgg tcacttcatc
     1381 ggattgacgc tgtcaattgc attccaaatc aacgcgttgc ctgaagtgga cggatgcggc
     1441 gatattgcta tccggcgctg caggggcgac aacgttgatt tatatgtagt ttgttaatgg
     1501 cagatagatt ttgatgctat cagcgtctat cggcgttcat cggcggctaa agaataaaac
     1561 tcaccgaccc gagcgagccg agctccatcg cgtcgataac cgcctcgcgc ttcgcgatct
     1621 tgagttgccc gccttcgacg cgcagctcgt agcggtaatg cccgacgtat tcgcggatgc
     1681 cctcgttgcg ccggaagcga tgaacggtga aattggcctc cacaccgagt gaggcgccat
     1741 tccggctgac gatgcggaca ttggatatca tgcggcgcgt gcgcgagggc gggtactcgg
     1801 cgtgggcgct cttgtccttc agccttgtca cgcgcgcgcg gatgcggcgg atgtcgtctg
     1861 cgatgatgaa cagcgtgtcc ttcgaatccg cgtccggccg gtcgttggag gggacgcggt
     1921 aaagggcgtc ttcggtcagg agcttcagcc agtcatcgag ccgccacgaa tcgaggaggg
     1981 cagcttcctc gtacaggaag tcctccactt gctggcgtgt cacctgagca cgacgggttg
     2041 tcatttcttc atgcgctcgg atatcagcaa ttagccgtca gccatcagcg gtcagcctgc
     2101 aacaaggagc gccttggctg aatgctgaaa gctgatcgct gaatgcttgc tccgcgcgtc
     2161 aaccttgacg ctcggagcgc atcagcttgt cccactgcac ccagaacgcc cgcaggtgcg
     2221 gctcatcggt gttgagctgt tcgccctcct tcgccatgcc gcgggttatc tccgaccacg
     2281 gcacttcccg gtgcgtgctc aggccggact ggaccgattc cagcacctcg atgtcgtccg
     2341 gcgtggcgaa accgcccggg ccgtagaagg tgagaaagct gtcgaggcgc accgcgcggg
     2401 cggtgggcga ttcctctttc ggcccgagcg cccaggcgag cacgcgcatg cggtcgaccg
     2461 ccaacgggta gaacgtccgg atggtaaccg aggagccgtc attcaggaac aggttcggga
     2521 agatgagcag attccggttg gtgtcggcga cccgggtggc gcgctcctca ccgaggcggg
     2581 cgaccagctc ggcgcggatg cgctcgatct cgggcttggc tgcttcgccg taaatcggga
     2641 tccagcgggc caccggccgc ccccggaaat tcacgttgtc ggtgcagccg tggccgttgc
     2701 cgagttcctt gcccacgccg tgcgccggga ggagatgacc cttctcgggg cgcttcatct
     2761 cgacccccga gctcttgagg taattgagcc acgacccgtg gacgctcagc aggtggtagt
     2821 cgtcgaagct gttctccgcc agcagcttcc agttggcgcg gatgtcatag tcctgcacgc
     2881 cctggatcac ctc
//
LOCUS       DBNGWZ010000198         3396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3420942, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000198 DBNGWZ010000000
VERSION     DBNGWZ010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3420942"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16CX_05725"
     CDS             complement(<1..391)
                     /locus_tag="AC16CX_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012047.1"
                     /translation="MRRASARRLPFLILWGLPAVGFVLLYQWAYFGSPVKHGFSDSLG
                     RFSEASGIGHLGLLVSPAKGLLVFTPLVAVAAVGVVAAFRRGERGLAFTLGAASLAHW
                     LFVGSWTEWHGGDSWGPRMMTDALPCIG"
     gene            complement(390..1130)
                     /locus_tag="AC16CX_05730"
                     /pseudo
     CDS             complement(390..1130)
                     /locus_tag="AC16CX_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1127..1924)
                     /locus_tag="AC16CX_05735"
     CDS             complement(1127..1924)
                     /locus_tag="AC16CX_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014486097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012048.1"
                     /translation="MKRPIAAPWSLLLPAALVALWHFAVDLRWVTEGIIPSPAQVLRS
                     WEIWIFGAAKASLSPYSGTWLDNVAYSTRRVLQGFGLAALVAIPLGLVIGWNRLVARL
                     VDPSIQLLRPVPITAWLPFAIAVFGIYDASALFLIGLGAFYPIVVNTTHGVRDTNLLL
                     LRAARMMGAGPWTVLARVVFPSALPAIFTGLRLGVGVAWTAVIVAEMIAVKSGLGYVL
                     WDAYYVGRMDICVATMFSVGVLGFLSDRVIVWTSRVLLRWRALEAHT"
     gene            complement(1932..2939)
                     /locus_tag="AC16CX_05740"
     CDS             complement(1932..2939)
                     /locus_tag="AC16CX_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012049.1"
                     /translation="MKAIPRLVFAVAVAALSAAAVAQGDLRVPLAQSLKIKQGMLNVP
                     ALSPLWLLPEEAAKYNIQVETVMFQRFADARTALVSGDIHITAFGPQDISLAVGQGAK
                     SLVGIAGVGSGNDCLIVRKGEDIKDWKTLSDKRIGVGAGSISWLKFAASVQENGLQYG
                     NLKIVNIVGGGANYLKALQGKDIDMAVVWQPFCAQAIVDGFGAYPAVDHNRSKTVGGL
                     IAVLAVNRPFMEKNRDAVQRLVVAYLDVLQVARADTVRWAKIYAEKAGLPENVAAESI
                     RITRLDPTLPLESIKRISKFLSDNGVITRDVSGEIAQHYTYDFLSKATGKSAEQLGLK
                     Q"
BASE COUNT          647 a         1118 c         1081 g          550 t
ORIGIN      
        1 ttccgatgca tggaagcgcg tcggtcatca tgcgggggcc ccaggagtcg ccgccgtgcc
       61 attcggtcca cgaccctacg aagagccagt gggccaggct cgcggcgccc agcgtgaacg
      121 cgagccctct ctccccgcgg cgaaacgcgg ccacgacgcc gaccgcggcc acggcgacca
      181 gaggcgtgaa gaccaggagt cccttggccg gcgagaccag gagccccaag tgcccgatcc
      241 ccgacgcctc cgagaagcgt ccgaggctgt cggagaagcc gtgcttcacg ggggagccga
      301 agtaggccca ttggtagagg agcacgaagc ccacggctgg gagcccccag aggatcagga
      361 agggcaggcg ccgcgcgctg gctctcctca cggatcagcg ccagcacctc gcgccgtagc
      421 acattgaact cgggcgaggt cgtgacctca atcgaacgcg gccgcgcgat tccgacagta
      481 atgctcttct tgatccgccc cggccgcgcc gtcattacgc aaacgcggtc cgcgagcagc
      541 agcgcttcct cgatatcgtg ggtgacgaac agtaccgtct ggtgatgccg ttcccagacc
      601 ccgagtagca gctcctgcat aagcgagcgc gtctgggcat cgaggctgcc gaacggctca
      661 tccatgagca gcacctgggg ctgcatgatc agcacgcgcg cgatgccgac acgctgtcgc
      721 atgccgccgg agagctccgc tggatagttt gcctcgaagc cgcgcagccc aacctgttcg
      781 atgatctggg tagcgcgcac gcggtattcg gcgaccggct ggccctgtgt cttgggaccg
      841 aacaccacgt tctccatgac cgacatccag ggaaacaacg cgggctcctg gaaaacgacc
      901 ccacgccgcg gcgaggggtc tgcgatggcc tctccaccga cacgcactgt cccgcgcgtg
      961 ggacggtcga aacccgcgac tatgttgaga agcgttgact tgccgcagcc cgacgggccg
     1021 agaatcgcga ggaattcatg cggggccgcg gtcaggtcga tttcctgaag cgcgattacg
     1081 cggcgcccgc ctttcgcgaa ttccctcgaa acgcccgtga gctcgatcac gtgtgcgcct
     1141 ccagtgctcg ccaccgcagc agcacgcgcg acgtccagac aatcacgcgg tcggaaagga
     1201 acccgagcac gccaacggaa aacatggtcg cgacgcaaat gtccatgcgg ccgacgtaat
     1261 aggcgtccca cagaacgtag ccaagtccgg acttgaccgc gatcatttcg gcgacgatga
     1321 cggcggtcca ggcgacaccc acgccgagtc gcagaccggt gaagatggcc ggcagtgccg
     1381 acgggaatac gacccgggcc agcacggtcc acgggcccgc gcccatcatg cgcgcagcgc
     1441 gcagcagcag caggtttgtg tcgcgcacac cgtgggtcgt gttgacgacg atcggataaa
     1501 aggcgccgag gccgatcagg aacagcgcgc tcgcatcgta gatgccgaag acggcgatgg
     1561 cgaaaggcag ccatgccgtg ataggcaccg ggcgcagcag ttggatcgac gggtcgacca
     1621 ggcgcgcgac gaggcggttc cagccgatca ccagcccgag cggaatcgct accagcgcgg
     1681 caaggccgaa tccctgcagt actcggcgcg tggaataggc gacgttgtcg agccaagtac
     1741 ccgagtaggg ggaaagcgat gccttggcag cgccaaagat ccagatttcc cacgacctga
     1801 ggacctgcgc tggcgacggg atgatcccct cggttaccca gcgcaggtcg accgcgaaat
     1861 gccaaagcgc taccagcgcc gccggcagga gcagggacca gggcgccgca atcgggcgct
     1921 tcaccgcggt gctactgctt caggcccagt tgctccgcgg acttgccggt tgccttggac
     1981 aggaagtcgt aggtgtagtg ctgcgcgatt tcgccggaca cgtcccgcgt gatcacgccg
     2041 ttgtccgaca ggaatttcga gatcctcttg atcgactcca gcgggagcgt cgggtcgagc
     2101 cgggtaatgc ggatggactc cgccgccacg ttctcgggca acccggcctt ctcggcgtag
     2161 atcttggccc agcgcacggt gtcggcgcgc gcgacctgca gcacgtccag ataggcgacg
     2221 accaggcgct gcacggcatc gcggtttttc tccatgaacg gccggttcac cgccagcacc
     2281 gcgatgaggc cgccgaccgt cttggagcgg ttatggtcca cggccggata agcgccgaag
     2341 ccgtccacga tggcctgcgc gcagaacggc tgccacacca ccgccatgtc gatgtccttg
     2401 ccttgcagcg ccttcaggta gttcgcgccg ccgccgacga tattgacgat cttcaggttg
     2461 ccgtactgca gcccgttctc ctgaaccgac gcggcgaact tgagccacga gatcgagccc
     2521 gcgccgacgc cgatgcgctt gtcggagagc gttttccagt ccttgatgtc ctcgcctttg
     2581 cgcacgatca ggcagtcgtt ccccgagccg acgcccgcga ttccgaccag gctcttcgcg
     2641 ccttggccga ccgcaaggga aatgtcctgc ggtccgaatg cggtgatgtg gatgtcgcca
     2701 gagaccagcg cggtgcgcgc atcggcgaag cgctggaaca tcaccgtttc aacctggata
     2761 ttgtatttcg cggcctcctc cggcagcagc cacaatggcg acagcgccgg cacgttaagc
     2821 attccctgct tgattttcag ggactgggca agcggcacgc ggagatcgcc ctgcgccacg
     2881 gcggctgccg aaagtgcggc aaccgcaact gcgaaaacca gcctggggat cgctttcatg
     2941 atggccttcc ttctcttgtg ggcgggaaag ggagaaatac tacatcagac cttgaagcgg
     3001 aagtggatca catcgccgtc gcgcgcgcct tagtcatatt gccttccagg cgcagcttcc
     3061 aggcttctct tgcgccctga tttccgttgc ggtcgacgtg aaccaagaaa aacatgggga
     3121 tagagcccat tcgcaagact gttcgcggtt gagcagtcgc actcaaggcc tcgaaatccg
     3181 caattgaaca gcccttgagg accgtaccct gttctgccag ctatttaagg ctgaacccct
     3241 gtttgcggac gaacgcgccg aagtccgcac gctcgggcac cgagtactgg cgcgccttgt
     3301 cctcggacca gccgtaaaat tccgcgtcgg gatagcggtc cgcgtagcgc tgcctgcggt
     3361 acggcacgag cgcgtgccgc cgccggtcgt aggcgt
//
LOCUS       DBNGWZ010000199        12846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_146856, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000199 DBNGWZ010000000
VERSION     DBNGWZ010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_146856"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..736
                     /locus_tag="AC16CX_05745"
     CDS             344..736
                     /locus_tag="AC16CX_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012050.1"
                     /translation="MRLTNWAAIVASVLALFVVGCATKPVYNVTNAPVATSKPNPSLD
                     DVGKAIMRAGAALGWQMKETKPGHMLGTLYLRKHVAVVDVNYSTTSYSITYKDSTELN
                     YDGNTIHNNYNGWIQNLEKGIHTQLSLL"
     gene            897..1163
                     /locus_tag="AC16CX_05750"
     CDS             897..1163
                     /locus_tag="AC16CX_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HIR3012051.1"
                     /translation="MVRQTELEGEVARLIIEAVNLERHPETVDPEAHLFADGLGLDSI
                     DMLEVALAVSRRYGFQLRSDGAGNERIFASLRALSAHIAQHRTR"
     gene            1244..1798
                     /locus_tag="AC16CX_05755"
     CDS             1244..1798
                     /locus_tag="AC16CX_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG4648 family protein"
                     /protein_id="HIR3012052.1"
                     /translation="MLEEPRWAAAGIALVACAVASFQYSRLVAAALGVVVLAAGLALA
                     AVAGSVLLYTPPLALNIALCVVFGGTLRSGREPLVSRFARIERGGDLPADLAHYARLV
                     TWAWTAFFALMAAISLALAAWGSAWSWSLFTNFVNYIFVAVFFSSEYLYRRVRFRHYA
                     HASPAELIRRLHTYRPFPRPADGN"
     gene            1871..3217
                     /locus_tag="AC16CX_05760"
     CDS             1871..3217
                     /locus_tag="AC16CX_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3012053.1"
                     /translation="MSTAYPLLRGFRADSTIGWSRGEPVNAARFSTTALAVAGMLPRK
                     RHVLNLCEDRLNFTLAVAAALIAGQVSLLPQSRAAGALRDLLANYPDSYCLADDNDLP
                     AGLPAIVLPSWSDAGSLGDVPSIPGDQVAQIAFTSGSTGHPQPHSRTWSSLVAGARFL
                     GRHLGIAPGADYSILGTVPAQHVYGFDTTVMLSLQNGVPLHSGHPLLPADIAAALRDL
                     PGKRWLATTPTHLRACVVEETQLPGLAGIICATMPLAPELVEAVERMWAVTIHEIYGC
                     TEAGVVALRRPGLGERYRVTSGMRLSKKGEETWIEGGHLSRALKLPDRIELASETEFT
                     LLGRPGDLVKVAGKRASLEALNWELLRIPGVRDGVFFVPDSLSGNEARLSALVVAPKL
                     DPGTILGTLRERIDPAFLPRPLLIVDALPRNATGKLPHEGVLALAQAAQLRGQRSA"
     gene            3217..3519
                     /locus_tag="AC16CX_05765"
     CDS             3217..3519
                     /locus_tag="AC16CX_05765"
                     /inference="COORDINATES: protein motif:HMM:NF047268.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApeI family dehydratase"
                     /protein_id="HIR3012054.1"
                     /translation="MLRVADEVGPAHPALAGHFPGNPVVPGALLLARVARAVAAAFGS
                     PVGTVLGTKFHAPLRPGERFEVEFERDAAGAVKFRVLRGATLIAAGNLSLNAASND"
     gene            3527..4426
                     /locus_tag="AC16CX_05770"
     CDS             3527..4426
                     /locus_tag="AC16CX_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR3012055.1"
                     /translation="MSAAWLAEPERGSPAAIGLITWLALKLGRSTTRWFLPPICLYYY
                     TFAPRSRRASRAYLARVLDREPGMRDVLRHYHAFASTVHDRVFLVCGHWKGFDIAVHG
                     AAEVDQHLARRRGCILLGSHLGSFEVLRSLGRFKHNYAINVLMHEANAGKTGKVLAQL
                     APELREHTITPGQPTTMLRVKECLDRGEIVGILGDRPLGGDRTQPCRFLGETARFPIG
                     PLLLAATLGVPVALFFGLYRGGAHYDVHFEMLSEGGTVERERRWEVAARWLESYVARL
                     EHYARAAPYNWFNFYDFWDAGAS"
     gene            4410..4994
                     /locus_tag="AC16CX_05775"
     CDS             4410..4994
                     /locus_tag="AC16CX_05775"
                     /inference="COORDINATES: protein motif:HMM:NF039988.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HIR3012056.1"
                     /translation="MRARVDQLIAASLLVLIPSPGVAATLSAQELMDMLSGTESSTAS
                     FLETKHSALLKAPLLLRGTLVYRRPDWLEKHVVHPYDERIIFDRGRLTIDNRTRNRKT
                     TLSVKTAPGLAALVESIRATRAGDLPALESHYQVKVDGHRGKWSMILKPLETQVAEHV
                     SAVTVSGTAARIVRIEVQEASGDRTVMDISEELK"
     gene            5015..7327
                     /locus_tag="AC16CX_05780"
     CDS             5015..7327
                     /locus_tag="AC16CX_05780"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3012057.1"
                     /translation="MAVWLLALAACGTWLYRNLAITTDLTVFLPPSTSPIQRVLIRQL
                     RDGVASRLVLIGLEGEAGPSLARASAELARRLRDSELFSYVNNGDSAALERDRAILFA
                     HRYLLSPAVGPGRFSATGLEAALRESLELLVSPAGPLFRRALPADPTGELRELLRFVV
                     PESGPEIRHGVWFSRDGVRALLVAETHAPGLDVEAQRRAIEAIREAHSASQPGKARLL
                     LSGPGVIGAQMRSQIEAEAWWLSALAAALALGVLFAVYRSPGIVAASALPVVSGLVAG
                     ITVVGLGFGAVHGITLGFGATLIGEAVDYPSYLFTQAASGERLEHTLARIGLTLRLAV
                     LTTVFGSLAMALSSFEGIAQLGVLTIVGVAAAGLTTWWVLPALVTPTFVRRRIPAAPF
                     DLPALADALNRARWLVIVLTIAALAVLAWRHDRLWDDNLANLGAIPETAKSLDRTLRG
                     DLGAPDVRYLVAARAPEREAALQKSELAALWLREAVSAGWVAGFDVPSNYLPSRRTQE
                     SRRAALPDPETLTRNLNTALKDLPFRDGLFVPFLEAIARARSGPMLEFEDLGDSMLRL
                     RVAALLINNEDGWTVLAPLRDVREPQRLAEAARQAGYEFLDLKVESERLVSGYRGETL
                     RLIALGLFCIVGLLAWGLRSFARTVRVITPILAAVVLDVALLVLSGKALSLFHLVALL
                     LVIGIGVNYALFFERPQADIAERARTQLSLIVCSATTLSAFGCLALSETPVLHAIGIT
                     VAIGCLLSLVCAAVLVGRAHGPDGLPPATR"
     gene            7398..8585
                     /locus_tag="AC16CX_05785"
     CDS             7398..8585
                     /locus_tag="AC16CX_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3012058.1"
                     /translation="MRPLVISHFATANPLGLGCSATLAALRERRGGLRHNDFESVGLD
                     TWIGRIEGLEDAPVPERLSAFDCRNNRLAQLGLRQDGFEQAVSRARNQYGAHRIAVLV
                     GTSTSGIHETELAYRERDPGSGSLPSWFRYRETHNVFSVADYVQRHLNLQGPAIAVST
                     ACSSSAKVFASAHRLIESGFADAAIVGGVDSLCLTTLYGFGSLELLSREPCRPCDAAR
                     DGISIGEAAGFALLERPEDAEGEVALLGYGESSDAHHMSSPHPDGEGAYVAMCEALDR
                     AGLAPSDVDYVNMHGTGTPANDRSEDQALYRLFGGSVGCSSTKGWTGHTLGAAGITEA
                     VISVLCLQHGYVPGSLNVRDVDPALKSRIVISPETRTLRNVLSNSFGFGGSNCSLLFG
                     KLP"
     gene            8582..9376
                     /locus_tag="AC16CX_05790"
     CDS             8582..9376
                     /locus_tag="AC16CX_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HIR3012059.1"
                     /translation="MSALYLQGAGVLGPGLPDWPAARRILSGEQTYQYGPPPQPNPDA
                     LPQTERRRGAKTVRWTIAVAQEALQGTDIQPAEVASVFASSSGDGETLHRICETLATP
                     ERDVSPTQFHNSVHNAAAGYWSIAAGSRLPSVSLCGHDVSFAVGLIEATAQVQTGKAP
                     VLLVTYDLPYPQPLLAARSIAEPLAVAVLLSREPRSCTLARWTVSLARGAVAIRLPKM
                     LKPEFATNPAVHALSLLAAVARRSAETVQVPYADGCHLRVKSSPWP"
     gene            9367..9801
                     /locus_tag="AC16CX_05795"
     CDS             9367..9801
                     /locus_tag="AC16CX_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR3012060.1"
                     /translation="MALSRDEFATLIPHRGAMCLLHEVLRVDDESITCRAVSHRDPAH
                     PLREQGVLPVVCGIEYAAQAMAVHGALKGSGALQSGVLVAVRDVALKVDRLDDIAEDL
                     ILTARRLLDNGGWLLYEFEVRAGARQLLSGRATVMLDAGGAR"
     gene            9798..10517
                     /gene="fabG"
                     /locus_tag="AC16CX_05800"
     CDS             9798..10517
                     /gene="fabG"
                     /locus_tag="AC16CX_05800"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3012061.1"
                     /translation="MKRALVTGGSGGIGAAICRELARQGLHVIVHAHRNAGRAEALAG
                     EIRSAGGSAETASFDVVDAARSAQELERLLKAGPVQVLVHNAGITADAPFAGMSESQW
                     SSVIGVSLNGFFNVAKPLLLPMMRTRWGRIVALSSVAGIMGNRGQANYAAAKAGLHGA
                     VKSLAIEVASRGVTVNAVAPGIIATGMTESVFTKEMIERLVPMKRAGTPEEVADLVGF
                     LASDRAGYISGQVVSINGGLI"
     gene            10702..11475
                     /locus_tag="AC16CX_05805"
     CDS             10702..11475
                     /locus_tag="AC16CX_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3012062.1"
                     /translation="MSKIAAVIPAYNEAGTIRDVATRTLYQVPMVIIVDDGSTDGTAA
                     ALEGLPVTLLRNAANCGKGASLVLAMFYALENGAEAVITLDGDGQHRPEDIPNLIAAH
                     VRNRDAIVIGSRLHDSQNIPTDRYAANRFANFWIAWAAGQPIPDSQSGFRVYPAALLR
                     VLNVRCDRASGFVYESEILIEAGRRGVKVVAVPVSAIYEVRGRRSHFRPVLDIARITR
                     MVAWKLLSRGLYLHGLVRSLRNNAHQDCLNDGSATRLIE"
     gene            complement(11708..>12846)
                     /locus_tag="AC16CX_05810"
     CDS             complement(11708..>12846)
                     /locus_tag="AC16CX_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR3012063.1"
                     /translation="RAAGKPWSFLHISKPEIDLPAGTHPYAPEVYAKAAENLNLMLRK
                     GILTRDPAPCYYAYRLIMGKHVQTGLVAAASVADYDINRIRKHEHTQTDKENDRVCQI
                     DALNAQTGPVMLAYPNAQEVDDILARCSAGAPDADAAADDGIRHSLWVVRDAEIQARL
                     TRVFDSMPALYIADGHHRSAAASRVAAARRAVNPRHTGEENYNYFLAVIFPHHQMQIL
                     DYNRVVSDLNGMDTGTFLARVRENFSVASSPTPVKPAKPAEFGLFLAGQWYRLVIHRE
                     LIPVNDPVARLDARLLSDHLLGPVLGIKDLRRDKRIDYVGGIRGLTELEKRVNSGEMA
                     AAFALHPTRMEDMMAVAEAGKVMPTKSTWFEPKLADGLVSHVLD"
BASE COUNT         2143 a         4067 c         4235 g         2401 t
ORIGIN      
        1 ctgcggcgga cgtcgcaggt ccacaacaga tacggcagcg agagcagatg cagcaacgca
       61 cccaggagcg cgccgggatt tctacaagtt tccccaatga atcggtgatg agtgacaagt
      121 gatgagtgga gttcaatcgt aacggccgcg tttttcacgc atcacccacc tctcgggatg
      181 tcgagtgcgc ccttatgacc ccaagcactt gatcttcccc tggactaaga ttagcctacg
      241 aatgacgcag gatttcaaat ccggtcgtcc gcgaggagac atagagagag gcggatgaga
      301 aaaacggaat gatggggaac agtgtcatta caagaggtca acaatgcgtc tcacaaattg
      361 ggcagcgatc gtcgcatcag ttctggcttt gtttgtggtt ggctgcgcga caaagccggt
      421 ctacaacgtc accaacgcac cggtcgccac ttcgaagcca aatcccagtc tcgatgatgt
      481 tggcaaagcc atcatgcgtg ccggcgcagc cctcggctgg cagatgaagg aaacgaagcc
      541 tggacacatg cttggaacgc tgtaccttcg caagcacgta gcggtagtgg atgtgaacta
      601 cagcaccacg tcctacagca ttacgtacaa ggacagcacg gaactaaact acgacggcaa
      661 taccatccac aacaactata acggttggat ccagaacctg gaaaagggaa tacacactca
      721 gttgagtctg ctgtagcaga acggtcgccc ggcgggcgca gcagttgacg ccggttgctc
      781 ttcccgatgg tcaggagacg ccggggagcg tgtatgccac acgcgggtcc ttgaatcaga
      841 accccccatg ctgaacgttg gcagtggctg gagcaacgaa gggggagaga atcaaaatgg
      901 tgcgtcaaac ggaactcgaa ggcgaagtcg cacggttgat catcgaggcg gtcaacctag
      961 aacggcaccc agaaacggtc gaccccgaag ctcacctgtt tgccgacgga ctcgggcttg
     1021 actcgatcga catgctggaa gttgcactcg cggtctcgcg caggtacgga ttccagctgc
     1081 gctccgatgg cgccggaaat gagcggatct tcgcctctct gcgtgccctg agcgcccata
     1141 tcgcgcaaca tcgaacccgg taacgcgttg catggcggga cggacacgtc tgctggctat
     1201 cgcggcgtgc tgcgcttacc cggtgctctc ccacgcagct gccgtgctgg aggagccgcg
     1261 ctgggcggca gccggcatcg ctctggtggc gtgtgccgtt gcgtccttcc aatatagccg
     1321 gctcgttgcg gccgccttgg gcgtcgtggt tctggcagcg ggtcttgcgc ttgcagccgt
     1381 cgccggcagt gtgctcctgt atacgccacc gctcgccctc aacattgcat tgtgcgtagt
     1441 gttcggcggc actttgcgca gtggccgcga gccactggtc agccggttcg cacgtattga
     1501 gcgcggcggc gacctgccgg ctgacctggc acactatgcg cggctggtta cctgggcgtg
     1561 gaccgctttc ttcgccctga tggcggcgat ttcgctggct ctggcggcgt ggggctcggc
     1621 atggtcatgg tcgctgttca cgaacttcgt gaactacatc ttcgtggctg tcttcttctc
     1681 gtccgaatat ctttaccggc gcgtacgctt ccgccattac gcccacgcaa gcccggccga
     1741 gctgatacgc cggctacaca cctaccgacc ctttccccgt ccagcagacg gtaactaggt
     1801 ccattcaatc atttcaggct gtccgatagt catggcagga ttacaattca tgctgaaacg
     1861 gcgctagccc atgtccactg cttatcccct gctccgcggt ttccgggctg atagcacgat
     1921 tggttggagc cgcggcgagc ccgtcaatgc cgcgcgcttt tccaccaccg cattggccgt
     1981 agccggcatg cttccgcgca agcgtcatgt cctcaatctg tgcgaggaca ggctgaactt
     2041 cacgctcgct gtggctgcgg ccctcatcgc cggccaggtc agcttgttgc cgcaatcgag
     2101 ggccgcagga gcgctacggg atcttcttgc gaactatccg gatagctatt gccttgccga
     2161 cgacaatgac ctgcccgccg gactgcctgc gatcgtgctt ccctcatgga gtgacgctgg
     2221 cagcttggga gacgtgccat cgatccccgg cgaccaggtc gcacagattg cgttcacatc
     2281 gggcagcacc ggacatccgc agccgcattc taggacttgg agttcgctgg tggcaggtgc
     2341 ccgttttctc ggccggcatc tgggcatcgc gccgggggca gactattcca tactcggcac
     2401 ggtccccgca cagcatgtgt atggcttcga caccaccgtg atgctctcgc tacagaacgg
     2461 cgtgccgctg cattcgggac acccgctgct gcccgcggac atcgctgccg cgctgcgcga
     2521 tctcccgggg aaacgatggt tagcgacgac gccgacgcat ctgcgcgcgt gcgtggtcga
     2581 ggagacacaa ttgccagggc tcgccggcat catctgcgcg accatgccgc tcgcacccga
     2641 gctggtggag gcggtggaac ggatgtgggc ggtgaccata cacgagatct acggttgcac
     2701 cgaggctggt gtggtggcgc tgcgccggcc cggattgggc gagcgctatc gcgtgacctc
     2761 cggcatgcgg ctctcgaaga agggcgagga aacctggatc gagggcggcc atctgtcacg
     2821 ggcgctcaag ctacccgacc gtatcgagct cgcaagcgag acggaattca ccctgttggg
     2881 ccggcccgga gacctggtca aggtcgccgg caagcgcgct tcgctggaag cgctcaactg
     2941 ggaattgctg cgaattcccg gggtgaggga tggcgtgttt tttgttcccg actcgctgtc
     3001 cggaaacgag gcacggctgt ccgccctggt ggttgcaccc aagctcgatc ccggtaccat
     3061 actcggtacc ttgcgcgagc gcatcgaccc cgcattcctg ccgcgtccgc tgctcatcgt
     3121 ggacgcgctg ccacggaacg ctaccggcaa gctgccgcac gaaggcgttc ttgcactggc
     3181 tcaggctgcc cagttgcgcg gccagaggag cgcgtgatgc tgcgcgtagc ggacgaagtc
     3241 ggcccggcgc acccggcgct tgctggtcat ttccctggca atccagtcgt acctggggca
     3301 ctgctgctgg cgcgtgttgc gcgcgcggtc gcagcagcct tcggttcccc ggtcggcact
     3361 gtgctgggga cgaaattcca tgcaccgctg cgtcccggcg agcgcttcga agtcgagttt
     3421 gagcgcgacg cagcgggcgc ggtcaagttc cgagtgctgc gtggcgcgac gttgatcgct
     3481 gcaggcaacc tgagcctgaa cgcggcctcg aacgactagc atctccatga gcgcggcatg
     3541 gcttgctgag ccggagcgtg gaagtccggc ggcgatcggc ctgatcacct ggttggccct
     3601 gaagctgggc cgcagcacaa cccgctggtt cctcccgccg atttgcctgt attactacac
     3661 ctttgcgccc cgctcacgtc gtgcctcgcg cgcctacctc gcacgggtgc tcgatcgcga
     3721 gccaggcatg cgtgacgtgc tgcgccacta ccacgccttc gcatctaccg tccacgaccg
     3781 cgtgttcctc gtgtgcgggc actggaaagg tttcgacatc gcggtgcacg gcgcggccga
     3841 ggtagaccag cacctcgccc ggcgccgggg ctgcatactg ctgggctccc atctcggcag
     3901 cttcgaggta ttgcgctcgc tcggccgctt caagcacaac tacgcgatca acgtgctcat
     3961 gcacgaagcc aatgcgggca agaccgggaa agtgctcgcc caactcgcgc ccgagttgcg
     4021 ggagcacacc attaccccgg ggcagcccac taccatgctg cgggtcaagg agtgccttga
     4081 tcgcggcgag atcgtcggca tcctgggcga ccgtccgctg ggcggagacc ggacccagcc
     4141 atgccgtttc ctcggggaaa cagcacgatt tcccatcggg ccgctgctgc tcgcggcgac
     4201 gctcggggtc ccggtcgcgc tgttcttcgg actctatcgc ggcggcgcgc actatgatgt
     4261 ccacttcgag atgctctcag aaggcggaac cgtggaacgc gagcgccgtt gggaagtcgc
     4321 cgcgcgctgg cttgagagct atgtcgcgcg gctggagcat tacgcgcggg ccgcgcccta
     4381 caactggttc aacttctacg atttctggga tgcgggcgcg agttgatcag ctcatcgcgg
     4441 cgtccctact cgttctcata ccgagcccgg gtgttgcagc gacgctgagc gcgcaggaac
     4501 tcatggacat gctctcgggc accgagagtt ccactgcgag cttccttgaa acaaagcata
     4561 gtgctctcct caaggcgccg ttgctgttgc gcggcacgct tgtgtatcgg cgcccggact
     4621 ggctggagaa gcatgtcgtg catccttacg acgagcgcat catcttcgac cgcggaaggc
     4681 tgactatcga caaccgcacg cgcaatcgca aaaccacctt gtcggtgaag accgctccgg
     4741 ggcttgctgc tctcgtggaa agcatccgag cgacgcgcgc aggcgatctt cccgctctgg
     4801 aatcccacta ccaggtaaag gtggacggcc atcgcgggaa atggtcgatg atcctcaaac
     4861 cactggaaac ccaggtcgcc gagcacgtga gcgcagtgac cgtgtcgggc accgcagctc
     4921 gcatcgtgcg gatcgaagtg caggaggcga gcggggaccg cactgtgatg gatatcagcg
     4981 aagagctcaa atgagctcga cgcggggcgt ggtattggcg gtctggctgc ttgcgcttgc
     5041 agcctgcggc acgtggctct accgcaacct tgcaatcaca actgacctga ccgtcttcct
     5101 tccaccctcg acgtcaccga tccagcgcgt gctcattaga cagctgcgcg acggcgtggc
     5161 gtcgcgactg gtgttgattg gtctcgaagg tgaggccggt ccttccctag cgcgggcgag
     5221 cgcagagctg gctcgtcggc tgcgggacag cgagctgttc tcgtacgtca ataatggcga
     5281 ttctgctgct ctggagcggg accgggcgat cctgttcgcc catcgctatc tgctcagccc
     5341 cgcggtcggc cccgggcgct tttcggccac tgggctcgaa gcggctctcc gggagagcct
     5401 cgagctactg gtttccccgg cgggccccct gttccgtcgg gccctgccgg cggatcccac
     5461 cggcgagttg cgcgaactgc ttcgattcgt ggtcccggag agcgggcccg agatacgcca
     5521 tggcgtctgg ttttcgcgcg acggcgtgcg cgcgctactg gttgcagaaa cccacgcgcc
     5581 gggacttgac gtggaagcac agcggcgcgc aatcgaggcg atacgcgaag cgcactccgc
     5641 gtcgcaaccg ggcaaggcgc gtctgctgct gtcgggaccg ggcgtgattg gggcgcagat
     5701 gcggagccag atagaggctg aggcgtggtg gctttcggcg cttgccgcgg cactcgcgct
     5761 cggcgtgctg ttcgcagttt accgctcacc cggtatcgtg gctgcgagcg ctctgccggt
     5821 ggtgtccggc ctcgtggccg ggatcacagt ggtcgggttg ggtttcggag ctgttcacgg
     5881 cattacgcta ggcttcggcg cgacattgat cggagaggct gtggactatc cgagctatct
     5941 cttcacccag gcggcgtccg gtgagcgact cgagcacacg ctcgcgcgca ttgggctcac
     6001 gctcaggctt gcggtgttga ctacggtctt tggctccctg gcgatggcgc tttcgagctt
     6061 tgagggcatc gcccagctcg gcgtactgac gatcgtcggc gtggcggctg ccgggttgac
     6121 gacctggtgg gtgttgcccg ctctcgtaac ccccaccttt gtccggcgca ggatccccgc
     6181 tgcgcctttc gacttaccgg ccctggccga cgcgctgaac cgtgcacgat ggctcgtaat
     6241 tgtgctcacg atcgccgcgc tggcagtcct cgcatggcgc cacgaccgac tgtgggatga
     6301 caatctcgct aatctgggtg cgattccgga gaccgccaaa tccctcgatc ggacgcttcg
     6361 tggcgacctg ggcgcgccgg acgtgcgcta tctcgtcgcc gcccgcgccc ccgaacggga
     6421 ggcggcgttg cagaaaagcg agttggcggc gctgtggctg cgcgaagcgg tgtcagccgg
     6481 atgggtcgcg ggatttgacg tcccttccaa ctatctcccg agccggagaa cgcaggagag
     6541 ccggcgggcg gcgttgcctg atccggagac acttacgcgc aacctcaaca ccgcgctcaa
     6601 ggacctgccg ttccgggacg ggctgttcgt gccgttcctt gaggccattg cccgcgcgcg
     6661 cagcggaccg atgctcgagt tcgaagacct cggcgactcc atgctcaggc tccgggtcgc
     6721 ggcgctgctg atcaacaacg aggacggatg gacggtgctc gctccgctgc gtgacgtgcg
     6781 cgaaccgcag cggctggctg aagccgcccg gcaggcgggt tacgaattcc tggatctcaa
     6841 ggtggagtcg gagcgcctgg tcagcggcta ccggggcgaa acactccggc tgattgcgct
     6901 gggcctgttt tgcatcgtcg ggctgctggc atggggcttg cgtagctttg cccggacggt
     6961 gcgcgtgatc acacccatac tcgctgccgt ggtcttggac gtcgcgctgc tcgtgctgtc
     7021 gggcaaggcg ctgtcactct tccaccttgt ggcgctgctg ctggtcatcg gcataggtgt
     7081 caactacgcg ctgttcttcg agcggcccca ggcggatatc gccgagcgcg cacgcacgca
     7141 gctttcgctc atcgtatgca gcgccacaac gctctcggca ttcggctgcc tggcgctgtc
     7201 ggaaaccccg gtgctgcacg cgataggaat caccgttgcc atcggctgtc tgctgtcgtt
     7261 ggtgtgcgcc gccgtactgg tgggtcgcgc gcacggcccg gacgggctgc cgcccgcgac
     7321 acggtagtgc actccgcgcg tggcggaacg cgcgcccgct tccagtatac ttacccggtt
     7381 cgccgcgggt gctgcgcttg aggccgctag tcatatccca tttcgccacc gcgaatcctc
     7441 tcggactcgg ttgctctgcg acgcttgcgg cgttgcgaga gcgccgtggc gggttgcgtc
     7501 acaacgattt cgagtccgtc gggctcgata cctggatcgg gcggatcgag ggcctggaag
     7561 acgctcctgt tccggagcgc ctgtccgcgt tcgattgccg caataaccgc ctggcgcagc
     7621 tcgggctgcg ccaggacggc ttcgaacagg ccgttagccg tgcgcgcaac cagtacggcg
     7681 cgcaccgcat cgccgtgctg gtcggaacga gcacgtcggg aattcatgag accgagctgg
     7741 cctaccggga gcgcgacccc gggtccggat cgctgccatc gtggtttcgc tatcgtgaaa
     7801 cacacaacgt cttctccgtt gcggactacg tccagcgtca cctcaacctg caagggccgg
     7861 ccatcgcggt ctccacggca tgctcgtcga gcgccaaggt ctttgccagc gctcaccgct
     7921 tgatcgagtc cgggtttgcc gatgccgcca tcgtcggcgg cgtggacagc ctgtgcctca
     7981 cgacgctgta cggtttcggc tcactcgagc tgctgtcgcg cgagccgtgc cggccgtgcg
     8041 atgcggcgcg agacggcatc tcaatcgggg aggcagccgg ctttgcgctg ctcgaacgcc
     8101 ccgaggacgc cgagggcgaa gtagcgctgc tcggctatgg cgaaagctcg gatgcccatc
     8161 acatgtcctc gccgcacccc gacggcgagg gcgcgtacgt ggcgatgtgc gaggcgctcg
     8221 accgagccgg gctggccccg agcgacgtgg actacgtgaa catgcacggc accggcactc
     8281 ccgccaacga ccgttcggag gaccaggcgc tctatcgtct gttcggcggc tcggtcggct
     8341 gcagttccac gaaaggctgg accggccaca cgctgggcgc cgctggcatc accgaggcgg
     8401 tcatctcggt gctgtgcttg cagcacggct acgtcccggg cagcctgaac gtgcgcgatg
     8461 tcgatccggc gctgaagagc cgcatcgtca tttccccgga gacgcgcacg ctgcgcaacg
     8521 tgctgtcgaa ctccttcggc ttcggcggca gcaattgcag cctcctgttc gggaagctcc
     8581 cgtgagcgcg ctgtacctgc agggcgcagg cgtgctcggc cccggcctgc ccgactggcc
     8641 ggcggcgcgg cgcatcctga gcggcgagca gacgtatcag tacgggccgc cgccgcagcc
     8701 caatccggac gcattgccgc agaccgagcg ccgccgcgga gcgaagacag tgcgctggac
     8761 cattgcggtg gcgcaggaag cgttgcaggg gacagatatc cagccggcgg aggtcgcctc
     8821 ggtgtttgcg tcaagctcgg gcgacggtga aacgctgcac cgcatctgcg agaccctggc
     8881 gaccccggag cgcgacgtct cgcccaccca attccataac tcggtgcaca acgccgccgc
     8941 cggctactgg agtattgcgg ccggatcccg cctgccctcg gtatcgctgt gcggccacga
     9001 tgtctcgttc gccgtggggc tgatcgaggc gaccgcgcag gtgcagacag ggaaggcccc
     9061 cgtgttgctc gttacatacg atcttccata cccgcagccg ctgctcgcgg cgcggtcgat
     9121 cgcggagccg ctcgccgtcg ccgtgctcct gtcccgtgaa ccccgctcct gcactctggc
     9181 gcgctggacc gtatcgctcg cccggggcgc ggtggcgata cgcctgccca agatgctgaa
     9241 gcccgagttc gccaccaatc cggccgtaca cgctctgtcg ttgctcgctg cggttgcgcg
     9301 gcgctccgcg gaaacggtgc aggtccccta tgcggacggg tgtcacctgc gggtcaagtc
     9361 aagcccatgg ccctgagtcg ggacgaattc gcgacgctga ttccccaccg cggcgcgatg
     9421 tgcctgctgc atgaagtgct gcgcgtggac gacgagagca tcacatgccg cgcagtgagc
     9481 caccgggacc ccgcacatcc gttgcgcgag cagggcgtcc tgccggtggt atgcggcatc
     9541 gaatacgccg cgcaggcgat ggcagtgcat ggtgcgctca aaggcagcgg agcattgcaa
     9601 tccggtgtgc tcgtcgccgt gcgggatgtc gccctcaagg tggaccgcct ggacgatatc
     9661 gccgaggatc tgattcttac cgcccgcagg ctgctcgaca atggcggctg gttgctctac
     9721 gaattcgagg tgcgcgccgg cgcgcggcag ctgctgagcg ggcgcgcgac cgtgatgctg
     9781 gatgccggag gcgcccgatg aagcgcgcgc tggtcacggg cggcagcggg ggcatcggcg
     9841 cggcgatttg cagggagctc gcgcggcagg gtctgcacgt gatcgtgcac gcgcaccgca
     9901 acgctgggcg tgccgaagcc cttgcaggag agatccgctc ggccggcggc agcgccgaaa
     9961 cggcgtcctt cgacgtagtt gatgccgctc gcagcgcgca ggagctggag cgtctgttga
    10021 aggccggccc ggtccaggtg ttggtgcaca atgctggcat cacggcggat gcgccgttcg
    10081 ctggcatgag cgagagccag tggtcgagcg tgatcggcgt ctcgctgaac ggcttcttca
    10141 acgtcgcgaa gccgctactc ctgcccatga tgcggacacg ctggggtcgc atagtagcgc
    10201 tgtcctcggt cgccggaatc atgggtaacc gcgggcaggc caactatgcg gcggcaaaag
    10261 ccgggctgca cggcgccgtg aagtcacttg cgatcgaggt tgcaagtcgc ggtgtcaccg
    10321 tgaatgccgt tgcgccgggc atcatcgcga cgggcatgac cgagagtgtc ttcaccaagg
    10381 agatgatcga acggctggtg cccatgaagc gcgccggcac gcccgaggaa gtggccgatt
    10441 tggtgggctt ccttgcttcc gaccgggcgg gctatatctc aggtcaggtc gtttccatca
    10501 acggtggact gatctgagcg ccgggacgga cagcggaggg acgtcccggc ccaacgagaa
    10561 ggccgaaacg cgctggccgg cgggccttct ctcgatagaa ttcctgcaag tacagtaaaa
    10621 tcctacctca ctagatttgc caacgagaaa ctcccaccgg acacacgccg gccgggctca
    10681 atccgctgac gaggggatcg cgtgagcaag attgcagccg tcatcccggc ctacaacgag
    10741 gccggtacca tccgtgatgt tgccacgcgt acgctctacc aggtcccgat ggtcatcatc
    10801 gtggacgacg gctccaccga cggcacggcg gcggcgctcg agggcctgcc ggtaacgctt
    10861 ctgcgcaacg cggccaattg cggcaaaggt gcgagcttgg tgctggcaat gttctacgcg
    10921 ctggagaatg gcgccgaggc cgtcatcacg ctcgacggcg atggccagca ccggcctgag
    10981 gacattccga atttgattgc ggcacacgta cgcaaccggg acgcgatcgt gatcgggtcg
    11041 cggttgcacg acagccaaaa catacccacc gaccgctacg ccgcaaaccg ctttgctaac
    11101 ttctggatcg catgggccgc cggacagccg attccggata gccagtccgg ctttcgcgtc
    11161 tatcccgccg cgctgctgcg cgtgctgaac gtgcgatgcg accgcgcctc aggcttcgtg
    11221 tatgagagcg aaatcctgat cgaggccgga cggcgcggcg tgaaagtggt ggcggtgccg
    11281 gtttcggcga tctacgaggt gagagggcgg cgcagccact tccgaccggt actggacatc
    11341 gctcgcatca cgcgtatggt ggcgtggaag ctgctatcac gggggctgta cctgcacggt
    11401 ctggtgagaa gcctgcgcaa caacgcgcat caggattgcc tcaatgacgg atcggctacg
    11461 aggctcattg agtaaaatgg accgcgtcaa atcacaggtt caagtgtctt gcgaagacaa
    11521 atttagcacg ccttggagcc cgatggagtt gatggcgtgc gtcgcccgcc ggttcacgac
    11581 gggcttggcg tgataggcga tggagacggc ggcttcgccc atgaaccgga agtcgttggc
    11641 gccgtcaccg accgcgataa cctgatctct cttgagttcc ggctcatcgc gcgctgttga
    11701 cagcgcctca atccagtacg tgcgatacca gcccgtccgc cagcttgggt tcgaaccagg
    11761 tggacttcgt cggcatgact ttgcctgctt cggcaactgc catcatgtcc tccatgcgcg
    11821 tgggatgaag ggcgaaggcg gcggccatct cgccgctgtt tacgcgcttc tccagttccg
    11881 tgaggccgcg gatgccgccg acgtagtcga tgcgtttgtc gcggcgtaaa tccttgatgc
    11941 ccagaacggg cccgagcaag tggtcggaca gcagccttgc gtcaaggcga gccacgggat
    12001 cgttcacggg aatcaactca cggtggatca ccaggcgata ccactggccg gcgagaaaca
    12061 ggccgaattc agccggcttg gccggcttca ccggcgtggg gctgcttgcc actgaaaagt
    12121 tttcccgcac gcgcgcgagg aatgtccccg tgtccatgcc gttcaagtcg gagacgacgc
    12181 ggttatagtc gagaatctgc atctggtggt gagggaagat cactgccagg aagtaattgt
    12241 agttttcctc tccggtgtgg cgcggattga cggcgcggcg cgcagcggcg acgcgcgagg
    12301 ccgccgccga gcgatggtgg ccatcggcga tgtagagggc gggcatggag tcaaagactc
    12361 gggtcagtcg tgcctggatt tcggcgtcgc gcaccaccca tagtgagtgg cggatgccat
    12421 cgtcggcggc agcatccgcg tcgggcgcgc cggccgagca gcgggccagg atgtcgtcca
    12481 cctcctgcgc gttcggataa gcgagcatga ccgggccggt ctgggcattc agtgcgtcga
    12541 tctggcacac gcggtcgttt tccttgtccg tttgcgtgtg ctcgtgtttg cggatgcggt
    12601 tgatgtcgta gtccgcaacc gaggctgccg ccaccaggcc ggtctgcacg tgcttgccca
    12661 tgatcaggcg gtaggcgtag tagcagggcg ccgggtcgcg ggtgaggatg cccttgcgta
    12721 acatgagatt caggttttcc gctgccttgg catagacttc aggcgcatag ggatgggttc
    12781 ccgccggcag gtcgatctcc ggcttggaaa tgtgcaggaa gctccatggc ttgccggcgg
    12841 ctcgcg
//
LOCUS       DBNGWZ010000200         2974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1783376, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000200 DBNGWZ010000000
VERSION     DBNGWZ010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1783376"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16CX_05815"
     CDS             <1..188
                     /locus_tag="AC16CX_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012064.1"
                     /translation="MYQYLRHPFTGVALCYKHGIPDAVMHVVATHSWEGDKFKRRPEA
                     IIFHHADFTDFDLERYK"
     gene            232..1506
                     /locus_tag="AC16CX_05820"
     CDS             232..1506
                     /locus_tag="AC16CX_05820"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3012065.1"
                     /translation="MKQCEVNMGQTFAEKILAKKAGLERTAPGQVVDITPDVALSHDN
                     SAPIYETFKKMGGERVFNPEMHFITLDHAAPPPSTQHAENHRVIRQLVKEQGLRYFYE
                     VGRGICHQVLVEEGLALPGEVVLGSDSHTPHAGVMGAFGAGIGRSEMASIWAIGVLWL
                     RVPESLKITVRGELNPGVTAKDLALKVIGDLGADGALGMSVEWHGDGIQGLPLNERAV
                     LPNMMAEMGCKNSYIPPDEVAFDYLKGRAKRPYTPIYPDPDAAYAREVEYDAATIEPM
                     VACPHSVDKVVPISEVAGTHVDQAFLGTCTNARIEDLAVAAEVMKGRTVAPGTRMIVI
                     PASSLVYIEALKAGYIQTFVEAGAVVESPGCGPCMGNHMGIPAVGEVTISAANRNFQG
                     RMGTKESEVYLASPAVVAASAVKGTIAHPRDL"
     gene            1511..2536
                     /locus_tag="AC16CX_05825"
     CDS             1511..2536
                     /locus_tag="AC16CX_05825"
                     /inference="COORDINATES: protein motif:HMM:NF017365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HIR3012066.1"
                     /translation="MRAQQVIYTWDYHHGQATRVIVGGLPPLRGATMAEKQAYFAEHC
                     DHIRASLMQEPRGHKNMLGAVLTDPATPDGDIGMLFLHPRGYFEMCGDSTFSAVAAVV
                     DSGIVTSEGSEGELSLKLDTVAGRVDVHVRLREGEPIAITFDNVASYGLGAQTINVEG
                     IGPVGTMLGYGGLTYAFVEARAVGVASLTRINRDELLDVGTRVWQSARAQTGLPSYVG
                     TDRIGEPRPVDLITLWEPLEGEQGARVANFYAPQTTGRTPSGTGLSARVAIEVAAGRL
                     REGETFIHESLLGLRFVARPVKTNIPRADGRTPGVVPAITARSFLMGTAQWVLHPEDP
                     FQYGFSF"
     gene            2538..>2974
                     /locus_tag="AC16CX_05830"
     CDS             2538..>2974
                     /locus_tag="AC16CX_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012067.1"
                     /translation="MKLQNSFILREYLRPQVLPTTWCPGCGLGIIMSAIAQAVHFRGL
                     DKDKVAMVSGIGCTGRMSGYVDFNTLHTTHGRAPAFATGLKLARPDMTVIVVMGDGDS
                     MSIGGNHLVHAMRRNIGLTAIVVNNSVYGLTGGQSSPTTPVGGR"
BASE COUNT          553 a          934 c          978 g          509 t
ORIGIN      
        1 gcatgtatca atatctgcgc caccccttca caggcgtcgc gttgtgttat aaacatggca
       61 ttcccgacgc cgtgatgcac gtcgtcgcca cccactcgtg ggagggcgat aaattcaagc
      121 gccgcccgga ggccatcatc tttcaccacg cagatttcac agattttgat ttggagcggt
      181 acaaataacg tatcacgctt cacgtttcac gtcatgcgtg atgcgtgaaa cgtgaagcag
      241 tgtgaggtca atatgggcca gacatttgcc gaaaaaattc ttgccaagaa agccgggctg
      301 gagaggacag cgccggggca ggtggtggac atcacgcctg acgtggcgct ctcgcacgac
      361 aactctgcgc cgatctacga gacgttcaag aagatgggcg gcgagcgcgt cttcaacccc
      421 gaaatgcact tcatcacgct cgaccacgcc gcgccgcccc ccagcaccca gcacgccgag
      481 aaccatcgcg tcatccgcca gctcgtcaaa gagcagggcc tccggtattt ttacgaggtg
      541 gggagaggca tttgccatca ggtgctcgtg gaagagggat tggcgctccc cggcgaagtg
      601 gtgctcggct cggactcgca caccccgcac gcgggcgtga tgggcgcatt cggcgcaggg
      661 attggccgca gcgagatggc gagcatctgg gccatcgggg tgttgtggct gcgcgtgccg
      721 gagagtctga aaatcacggt gcgcggagaa ttgaatccgg gcgtgacggc gaaagacctc
      781 gccctcaaag tcatcggcga tctaggcgca gatggcgcgc tggggatgtc ggtcgagtgg
      841 cacggcgatg gcattcaggg attgcccctc aacgagcgcg ctgtcctccc caacatgatg
      901 gccgagatgg gttgcaagaa cagttacatc ccgcccgacg aagttgcttt cgactacctc
      961 aaaggccgcg ccaaacgccc atacacgccg atctatcccg accccgacgc cgcgtatgca
     1021 cgcgaggtcg agtatgatgc ggcgacgatt gagccgatgg tcgcctgccc gcatagcgtg
     1081 gacaaggtgg tgccgatctc tgaggtggcc gggacgcacg tggatcaggc tttcctcggc
     1141 acctgcacca acgcgcgcat cgaagacctg gccgtcgccg ccgaagtgat gaagggccgc
     1201 acggttgcgc cgggcacgcg gatgatcgtc atcccggctt cgtcgctggt ctacatcgag
     1261 gcgttgaagg ccggttatat tcagaccttc gtcgaggcgg gcgcggtcgt cgaaagtccc
     1321 ggctgcggcc cgtgcatggg caaccacatg ggcatcccgg cggtcggcga ggtgaccatc
     1381 agcgccgcga accgcaactt tcaagggcgc atgggaacga aggaatctga agtgtatctc
     1441 gccagcccgg cggtcgtcgc cgcgtcggcg gtgaagggga cgatcgcgca ccccagggat
     1501 ttgtgacttc atgcgcgcgc agcaggtcat ttacacgtgg gattaccatc acgggcaggc
     1561 cacccgcgtc atcgtcggcg ggcttccgcc cctgcgcggc gcgacgatgg ccgagaagca
     1621 ggcgtacttc gccgagcact gcgatcacat ccgcgccagc ctgatgcagg agccgcgcgg
     1681 ccacaagaat atgctcggcg cggtgctgac cgacccggcg accccggacg gcgacatcgg
     1741 gatgctgttt ctccacccgc gcggctactt cgagatgtgc ggcgacagca ccttcagcgc
     1801 cgtggccgcc gtggttgatt ccggcatcgt caccagcgag ggatcggagg gtgaactgtc
     1861 gctgaaactt gatacggtcg ccgggcgggt ggacgtgcac gtccggttga gagagggcga
     1921 gccgattgcg atcacgttcg acaacgtggc ctcctacggc ctcggcgcgc agacgatcaa
     1981 cgtcgagggc atcggcccgg tggggacgat gctgggctac ggtgggctga cgtatgcgtt
     2041 tgtggaagcg cgcgcggtgg gcgtcgcttc gctgacccgc atcaaccgtg acgagttgct
     2101 ggacgtcggc acacgcgtgt ggcaatcggc gcgcgcgcag accggcttgc ccagctacgt
     2161 tgggacggac cgcatcggcg agccgcgccc ggtggatttg atcacgctgt gggaaccgct
     2221 ggagggagag cagggggcgc gcgtcgcgaa cttctacgcg ccgcagacca cggggcgcac
     2281 gccgtccggc acggggctgt ccgcgcgggt ggccattgaa gtagccgccg gccgacttcg
     2341 cgaaggggaa acattcattc acgagagtct gctgggcctg cgattcgtgg cgcggccagt
     2401 caagacgaac atcccgcgcg cagatggcag gacaccaggc gtcgtgcccg cgatcacggc
     2461 gcgttcgttt ttgatgggca cggcgcagtg ggtgcttcat cccgaagacc ctttccaata
     2521 tggattcagc ttctagaatg aaactacaaa actccttcat cctccgcgaa tatctccgcc
     2581 cgcaagtcct gccgaccacg tggtgccccg gctgcgggct gggcatcatc atgagcgcca
     2641 tcgcgcaggc cgtccacttt cgagggctgg acaaggacaa ggtggcgatg gtctcgggca
     2701 tcggctgcac cgggcgcatg tccggctacg tggacttcaa cacgctccac accacgcatg
     2761 gccgcgcgcc cgcgttcgcc accggcctca agctcgcgcg cccggacatg acagtcatcg
     2821 tcgtgatggg cgacggcgac tcgatgtcca tcggcggcaa tcatctggtg cacgccatgc
     2881 gccgcaacat cggcctgacc gccatcgtcg tcaacaacag cgtctacggc ctcacgggcg
     2941 gccagtcgtc gccgaccaca cccgtcggcg ggcg
//
LOCUS       DBNGWZ010000201        17711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3421178, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000201 DBNGWZ010000000
VERSION     DBNGWZ010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3421178"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC16CX_05835"
     CDS             <1..864
                     /locus_tag="AC16CX_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HIR3012068.1"
                     /translation="FARRPVAEQSGLKAQLEALVALAIRPLPAANRVVLDTSDGVTVV
                     TLGDPQAALELAERSQAAAADLPLCIGVNHGPVMAATDAVRGAGLVGDGLTAGMTLAN
                     VATPGRFLASRAFHDALEAHAPGRAADLSSVGVFTDPNVRTHELYALDQQASRARRRR
                     LMLAGALSVIVILGLGIAARVILQGMAARPAVIVFEITPRGDVVIDGVVKGRTPPLTE
                     LEVSPGHHTIEIRNSPYPPLRLDVNLSPEEEMTIRHSFARPKSAQPKSTEPKSKSASD
                     YLRDLRRRLGF"
     gene            1166..2377
                     /locus_tag="AC16CX_05840"
     CDS             1166..2377
                     /locus_tag="AC16CX_05840"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3012069.1"
                     /translation="MSTPALTRLGRYRIVAELGRGAMGVVYKAEDVQLNRTVAIKTII
                     MSADPAERAEYEARFNQEARAAGGLNHPNLITIHDVGREGDIVYMAMELLEGVELREL
                     MRTGRLPLPLTLDIMAQAAEGLAYAHEHGVVHRDIKPGNIMVVRGRHAKIMDFGIARM
                     QVSDIKTQTGAILGSPKYMSPEQVAGRPVDHRSDIFSLGVVLYEAAAGTAPFNASAVS
                     QLMYQIANAPHSPPSAVNPSLPAMLDLIAAKALEKSCEGRYQSAAELAADLRACLKDL
                     DIAQNVPSASSEATVRLDAGAASTVKLDGQIAKTQAGGTAAVRSGGVGVTTNAGAFFS
                     LSRRFDSSAAMQRFTQLATAGGADAQAATLPPSSGAQAVLSRMWRDPGRRIVAAAVIA
                     AIAIAVAIVLV"
     gene            2471..2791
                     /locus_tag="AC16CX_05845"
     CDS             2471..2791
                     /locus_tag="AC16CX_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012070.1"
                     /translation="MRRIGYVAALAILLSVPVLGPAQEKKPAAVKGEAPAAKPAAKPA
                     AAKVEPASEKPAAKMKVAAKAEPGGEKPAPAKRGPSRANEDARSCLQFPTNLEIHKCA
                     EQYR"
     gene            complement(2923..3081)
                     /locus_tag="AC16CX_05850"
     CDS             complement(2923..3081)
                     /locus_tag="AC16CX_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012071.1"
                     /translation="MCDDYQEKLYWIRRMEQMRKEEELKKQSGSPKPVKPAAPAEADV
                     KEHEPVPA"
     gene            3359..4288
                     /locus_tag="AC16CX_05855"
     CDS             3359..4288
                     /locus_tag="AC16CX_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3012072.1"
                     /translation="MSEPIQIRMGGYGPPSTTCSRGLKVMGDRLAAEFGSAVDVKYIW
                     NILDFGYKAGDLLWMAEHGILTLTYQSTSYLADRVPELEFADLPFLFDGLPEARAAMD
                     GKLGAWLSRKIEERIPGYRVLGYFENGYRHVSNRLRPVRAPADLKGMRIRLMPGKVHA
                     RSFELLGAIPFSLDLKPGLEAIVSGAVDAQENPLANTVDYGAHKVHRYHTLTGHCYLS
                     RGIYLNRAEFERWPRELQEGIQRAAREAIAAQRELAVEEEQTARKSIVIAGGEVLELT
                     PEERAAFARLVKPLHDEARSRFGEAVFAFAKTP"
     gene            complement(4540..7230)
                     /gene="acnA"
                     /locus_tag="AC16CX_05860"
     CDS             complement(4540..7230)
                     /gene="acnA"
                     /locus_tag="AC16CX_05860"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3012073.1"
                     /translation="MTPSFNTIARREIDLGPSRKYAYYSVPALERHGIGPVSRLPVSL
                     RVILESLLRNCDGKRVTERQVRALANWRPNGRREEEVPFIVGRVVLNCAAGIPLLGDL
                     TAIRGAVRRMGYPGEIVGPKVPVDMALDHTLTVDYHGTPDALARNMELEIQRNHERFR
                     FVKWAMQAYKGIRLMPPGWGILHQLNLEFLAPGYLEKDGICYPDTLVGTDSHTCMIAG
                     LGVVGWGVGGIEAEAAVLGQPVYYLTPDVVGVHVTGALEHGVTATDLVLHVTEMLRKA
                     KVVGQFVEYYGEGVKNLTLPDRATLSNMAVEYGATIGYFPVDGQTCRYLLQTGRSEER
                     VWAVEAFYRAQGCFGAAREGEVDYSRVLQLDLGTVEPNVAGPKRPQDRIPLREIKSRF
                     ESVLTQPVAGGGYGKAAVRSRSNGEVDHGDVVIAAITSCTNTSNPGVMLAAGLVAKKA
                     VERGLKAKPWVKTSLTPGSTVVSKYLAATGLQRYLDRLGFAIAGYSCGTCVGASGPID
                     AGLEKTITEKDVVACAVLSGNRNFEARIHPAVRAAFLASPPLVVAFALAGRVDIDLAK
                     EPLGCTDKGEPVHLRDVWPTAEELQQALEVAANPAFYRETYAADIASRNPLWKTIPLA
                     TGEMYPWESDSSYIRQPPFLDEELRLSTLTDIKSARALAILGHSITTDHISPIGSIKS
                     TSPAGLYLQKLGVAPADFNNYGARRLNHEVMMRGTFANVRLKNLMVPGVEGGVTAHQP
                     SGERMSIYDAAMRYQSERVPLIVVAGEEYGTGSARDWAAKGTRLLGVRAVVAGSFERI
                     HRSNLVGMGVLPCQLPGGTTAATMGLNGSEVFDLIGLDDDVKPGQALMLVVHRPDGHT
                     ENITVTLRLDTPAEMDYVRHGGIMPYVLNEITKGVKAAAG"
     gene            complement(7257..8669)
                     /locus_tag="AC16CX_05865"
     CDS             complement(7257..8669)
                     /locus_tag="AC16CX_05865"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3012074.1"
                     /translation="MKAETLPKQGAAQELIQFCQALSLETAPGSVVEYAKWCLLDSLG
                     CALFGSRQPWARIMAEEMLAEGSRGRSSILGRNETLAAPAAALCNGTATHGFELDDLL
                     DEAIVHPGATVVPAALAAAEAIDAPGSRLLLGIIAGYEAINRVGLALGVEAARRGHHQ
                     TALAGPLGAVMAAGVVMNLSRAQLTTAVGLACSTSSGTKSFAAGTGGGMMKRMHAGRA
                     AEAGVRMAQLAARGFTAPPTALDGPFGLLEVFAGKSARPEQLALDLGRRWAVEQVYVK
                     VYPCCAWIQATVQQLVGMRGARPVKAEEVKKLRLGVCSYAARNNGNVAPPDTMGAQYS
                     LPYCAALAVTADPRDPAMYFGDQLDEPSRRGLARRIEIVVDPEMEAAYPKHYGARVHL
                     ELANGDRHDSAVLDPHGMPSDPCSDEERIAKFKLLARHCMSDARASQLIDAVRKVENL
                     RSAGELTRAMRLDQRDLAAV"
     gene            complement(8689..9075)
                     /locus_tag="AC16CX_05870"
     CDS             complement(8689..9075)
                     /locus_tag="AC16CX_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012075.1"
                     /translation="MSEAPWVFQLHGDARGIARKLGEGIEARIFPGERVMLSVVRVEP
                     NSSGTIHSHPEEQWGVLLEGRCTRIQNGEEVEATAGDFWYTPGGVSHGVRTGATGALI
                     LDIFSPPRAEYRQAGEGFGTAATQAS"
     gene            complement(9095..10540)
                     /locus_tag="AC16CX_05875"
     CDS             complement(9095..10540)
                     /locus_tag="AC16CX_05875"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR3012076.1"
                     /translation="MKQDLFFKRLIAEIAKTASAGKKLTLGETVDLAESLGVRMSDIV
                     IAEAMAAHDMTHDEVIRAVLDAFAFNLLALEIGLGRGNSFLLGSIGRELQECRTRGTA
                     VIGDRFVDKAVVYTIATEVGNHEVGLQPCAGTGDSCVYTGLLKALLETRLSRLQVGRL
                     AALMVKIGTFFKEGKKTTGCNMEGFGAGAACTAAALAEMRKGSPRQVAKAIVLAISPT
                     IAVPCTPRVIVPGLCATHIGGAVLIGNLAAHLALKTSIAVPIDVDVMLAMAANIHAAA
                     APVITKINLDYMRPYFKTEPVVDGFVAGSVLAAEQESGRSVLARAREEIRKFAMSANP
                     LISPFGEAVVGGSSIAVGSPTNMGRIAHELAGGEVKKITIELTSDLFARRAINVPGIL
                     MGAVLGASTKDIKAYHNVLKEIARRRIAVEIRHVTEPEVQRIHIEARDKSAFVDAMNR
                     GGGRLKLVDARPSLAEAQTAAVRLGIKLAEV"
     gene            complement(10590..11570)
                     /locus_tag="AC16CX_05880"
     CDS             complement(10590..11570)
                     /locus_tag="AC16CX_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012077.1"
                     /translation="MFAEPRKLAATAIAIAMTGVFSASAQTYPSKPIRFVVNFAPGGG
                     TDIVARAMAPEFTKTLGQQVIVENRAGGNGNIGADVVAKAAPDGYTVLVATNAAIVIN
                     PHLYKKLSYDPLRDLAPVSQIGTLPFVLATHPTVPARSVKELIALAKSTPKGLTYSSS
                     GTGGGAHLAGEMLKNMAGINITHVPYKGGAPAIMALLSGEVDFMFISIFTVTPMLKDK
                     RLRAIAVTSATRNKALPDVPAMAELPILKGAESDLWYGMLAPANTDRKIIDILARETR
                     RVLLLQEFRDRFEPTGTVLVGSSPDDFGKTIKADYDRWKQVVRTSGATLE"
     gene            complement(11589..12095)
                     /locus_tag="AC16CX_05885"
     CDS             complement(11589..12095)
                     /locus_tag="AC16CX_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3012078.1"
                     /translation="MRFDLNRVIRGRIWKFGDSVDTDSINPFYKYSTMEELKQHTLES
                     FRPEFPKEVKPGDLIVGGRNFGCGSHRPATVLREVGVAAIVVESASRLFLRNTIALPM
                     PIYVAPGIAGIVQDGETLEIDYRAGCVKNVATGRTIPIPRFPPMIERIYECGGLPQLA
                     RQRYLAET"
     gene            complement(12085..13362)
                     /locus_tag="AC16CX_05890"
     CDS             complement(12085..13362)
                     /locus_tag="AC16CX_05890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HIR3012079.1"
                     /translation="MGQTIAEKILSRHNLAQAPVKAGDFIGARIDGAMCHYQFMDIHR
                     LAVEAGFEEGFPRVWDTERVYLLVDHHQPALAQSYADENVLIRREAKRLGIKHFHDAE
                     PGIAHQMMPDYGYVRPGELVVGNDSHTIAYGALNAGATGITRADMLHVLLFGELWFQV
                     PRTIKVVLAGRQPNYPIAKDIILHLAGLHGDDFAQGMAIEFTGPLVRELSIDSRMCLS
                     THGVEVGAKFAVFPFDGRTREYLRTRTDRPYEPVAADADAVYENEITLDVDEMPFAVA
                     KPHTFGNVSPVNEVAGIKIDQAQIGSCANGRFEDIEIAARMLKGRKVAKGVRFLISPS
                     SQQVYLECMKAGLIQQLVESGAHVVTPGCGICQPKVGYVSAGEVCITATTRNFRGRKG
                     SLDAQLYLAGPLTVTAAAVAGKIVDPKEVFDEI"
     gene            complement(13373..14335)
                     /locus_tag="AC16CX_05895"
     CDS             complement(13373..14335)
                     /locus_tag="AC16CX_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012080.1"
                     /translation="MSLFTRFLLLSLACVALAHAQTYPSRPVRIIVGFVAGGPSDLVA
                     RMVGQGLSERMGQQFLVENRPGATGTIGAELVAKAAPDGHTLYLASQTTHAVAPALLP
                     KVGYDPIKDFVTVVRVAHNPLLMVTHPSMPVRSFKDLVALAKARPGQINFATGGIGSS
                     PHMSMELVKSITKIDMVPVHYKGDGAAIIDILGGHVQLFNASIATLLPHVRTGKMRGL
                     AVTGLKRSAMAPEFPTLAESGLPGFEVLTWFGILATAGTPREIVNRLNKEILQVVAQP
                     AVKEQLLKLGFEVLTNTPEEYAAYLKQEHARWGKVIRDLGLKAE"
     gene            complement(14392..15894)
                     /gene="tcuA"
                     /locus_tag="AC16CX_05900"
     CDS             complement(14392..15894)
                     /gene="tcuA"
                     /locus_tag="AC16CX_05900"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3012081.1"
                     /translation="MERTETRYDVIVVGGGNAALCAAISASDEDAKVLLLERAPEEER
                     GGNSSFTEGLLRCVYNGPDDIRALSPDLSEEEMKSDFGTYTEEKFFDDMARITQNRTD
                     PDLCEILVRGSNPTMHWLKDKGVRWIPQFGRQSFKIGGKYTFWGGATLAASGGGRGLI
                     DTLYKAAEKAGVQVHYNAWVRDLLHSDAGVQGVVARLKGATRDIRARSVILACGGFEA
                     NAEWRSRYLGKGWDLAKVRGTRFNTGDGLAMALRIGAQPCGHWSGCHAVGWERYASDF
                     GDFSTVDDYERDSYPFSIMVNADGRRFLDEGADIRNYTYAKYGRIILEQPGQFAWQIF
                     DSSVTHLLRPEYRSKHVTKVKADSLEELADKLEDVNKEQLLKTIREFNAAVKRDVPFN
                     PNVKDGRAAVGLCVPRSNWAIPLENPPFEAYAVTCGITFTFGGLKVTTDAQVIDTNHQ
                     PIAGLYAAGEMVGGLFYFNYPGSTGLMSGAVFGRLAGRSAAMAAKLAKAA"
     gene            complement(15913..17088)
                     /locus_tag="AC16CX_05905"
     CDS             complement(15913..17088)
                     /locus_tag="AC16CX_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3012082.1"
                     /translation="MAVIDRIEIHVTGLPVRVQRIFSSGSYDTGPAERLLSKPVLVKI
                     HADGVVGCAQIRPISPGHFVADTTHSVVAAVKEIYGPALIGKSIFDIEAINEAFDARL
                     ANNPAARAVLDIAVRDAMGKATNQPLYNLLGGLCQPRIPLEWSVSMAEETATMVAEAK
                     RAVDEFGIRVLCLKMADRRGWRHDVRNFQAVRKAVGDGVMIGVDPNTGWTLADALSAI
                     RELKKMDLGYIEQPIARRDLKGMAEIRRAADGVPVMADEALFTIQDAYALAEARAVDA
                     YCIKLYKLGGFTPAKKIAAIAEAANIQLNCGGLAVQSQLEAAAGAHYYASTPASRMMG
                     AAEFVFGLNAVGKDPLVPETDFVARDGHVDVPRGPGLGISIDEAALKKNTLLYERVD"
     gene            complement(17116..>17711)
                     /locus_tag="AC16CX_05910"
     CDS             complement(17116..>17711)
                     /locus_tag="AC16CX_05910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3012083.1"
                     /translation="QTNFRSSKEVKQPDLVLRLLPVSGKDRYARTKAYGLDPFPGFTF
                     GITPLQPRSVGTLHIRSRDPHDQAAMDPRYLSHEADAQLFLDGIRVARKVARQPALKS
                     LIVRETRPGPETNDNPALLDYVRETVQTSWHMVGTCRMGVDDTAVVDPELRVRGIASL
                     RVVDTSICPTIPSSNTNAAAIAIGEKGADMVLAAARV"
BASE COUNT         3003 a         5848 c         5573 g         3287 t
ORIGIN      
        1 ttcgcccgcc ggccggtggc ggagcagagc gggctcaagg cgcagctcga ggcgctcgtt
       61 gcgctcgcga ttcgccccct gcccgcggcc aaccgcgtcg tcctcgatac ctcggacggg
      121 gtcacggtcg tgacgctcgg cgatccccag gccgcgctgg aactcgcgga gcgctcgcag
      181 gcggcggccg ccgatcttcc actctgcatc ggggtcaacc atgggccggt catggccgca
      241 accgatgcgg tgcgcggggc cgggctcgtt ggcgacggcc ttaccgccgg catgacgctg
      301 gcgaacgtcg cgacgccggg ccgcttcctc gcgtcgcgcg cgttccacga cgcgctcgaa
      361 gcccatgcac ccggccgcgc cgcggacctc agctcggtgg gcgttttcac cgaccccaac
      421 gtgcgcacgc acgagctgta cgcccttgat cagcaggcct cgcgcgcgcg ccggcggcgg
      481 ttgatgttgg ccggcgcctt gtccgtgatc gtcatactcg gcctgggcat cgcggcccgc
      541 gtcattctcc agggcatggc ggcgcgcccc gccgtcatcg tgttcgagat caccccgcgg
      601 ggcgacgtcg tcatcgacgg cgtcgtgaag ggcaggacac ctccgctcac ggagctggag
      661 gtgagccccg gccatcatac gatcgaaatc cgcaacagcc cgtatccgcc gctcaggctc
      721 gacgtgaatc tcagtcccga ggaggaaatg accattcgcc actcgttcgc ccgcccgaag
      781 agcgcccagc ccaagagcac cgagcccaag agcaagagcg ccagcgatta cttgcgcgac
      841 ttgcgccggc gtctcggctt ctaaaagcgc caacgcaagc tggctcgaaa acgagaatga
      901 aaccgcagat caacgcagat gcacacggat aactcacttt ccgaagatgt tattggcaag
      961 gagatccatc cgcgtttatc cagcgctgtc ttttggtctc ttgctttctt ctataaaaat
     1021 gcgcctggac ttgtttcaac aatcaagaat agagtgcatc cgcagaaatc cttgatgaac
     1081 gccgatacca accagaacca atacggggac ctatttcaat gtatttgcgt ttatcggcgt
     1141 tcatcggcgg tttcccacgt ttgacatgag cacgcccgcg ctgacccgtc ttggccgcta
     1201 ccggatcgtc gccgaactcg ggcgcggcgc catgggcgtc gtctacaagg cggaggacgt
     1261 ccagctcaac cgcacggtcg cgatcaagac catcatcatg tcggcggacc cggccgagcg
     1321 cgccgaatat gaagcgcgct tcaaccagga agcccgggcc gcgggcgggc tcaaccatcc
     1381 caacctcatc acgatccacg acgtcggccg cgagggcgac atcgtgtaca tggcgatgga
     1441 attgctcgag ggggtcgagc tgcgcgagct gatgcggacg ggccggctgc cgctgccgct
     1501 cacgctcgac atcatggcgc aggcggcgga gggccttgcc tatgcgcacg agcacggcgt
     1561 cgttcaccgc gacatcaagc cgggcaacat catggtcgtg cgcggccggc acgcgaagat
     1621 catggacttc gggatcgcac ggatgcaggt ctccgacatc aagacccaga ctggagcgat
     1681 actcgggtct ccgaaataca tgtcgcccga gcaggtggcc gggcggcccg tggatcaccg
     1741 ctccgacatc ttctcgctcg gcgtcgtgct ctacgaggcc gcggccggca cggcgccgtt
     1801 caacgcgtcg gcggtgagcc agctcatgta ccagatcgcg aatgcgccgc acagcccgcc
     1861 gagcgcggtc aacccgtcgc tgccggcgat gctcgacctg atcgccgcca aggcgctgga
     1921 gaaatcgtgc gaggggcgct atcagagcgc ggcggagctc gcggccgatc tgcgcgcgtg
     1981 cttgaaagat ctggatatcg cccaaaacgt tccgtccgca tcgagcgagg ccaccgtgcg
     2041 gctcgatgcg ggcgctgcga gcacggtgaa gctcgacggg caaatcgcga agacgcaggc
     2101 gggcgggacc gcggccgtga gatccggggg cgtcggcgtc acgactaacg ccggggcatt
     2161 tttttccctg tcgcggcggt tcgactccag cgcggccatg cagcggttca cgcaactggc
     2221 gactgcgggc ggggccgacg cgcaggccgc cacgctccca ccgtcgtctg gcgctcaagc
     2281 cgtcctgtcc cgcatgtggc gcgatccggg ccggcggatc gtcgccgcgg ccgtcatagc
     2341 cgctatcgcc atcgccgttg cgatcgtgct tgtctagcgg aatggggtcg gattcacgtg
     2401 tgacttattc cgcattttgc gatcacgccg ctacgccact atcatgtttt tctttgaaag
     2461 gaaccgacct atgagacgca tcggctacgt ggcagctctc gcgatactgc tctccgtgcc
     2521 tgtactcggc ccggcgcagg agaaaaaacc cgcagcggtg aagggagagg cgccggccgc
     2581 gaagccggcg gcgaagcctg ccgcggcaaa agtcgagccg gcgtccgaaa agccggcggc
     2641 caaaatgaag gtggcggcga aagccgagcc gggaggagag aaaccggctc cggccaagcg
     2701 cgggccgtcg cgggcgaatg aagatgcgag aagctgcctg cagttcccca cgaacctcga
     2761 gatccacaag tgcgccgagc agtaccgata gctggataag agaagcgctt ggaatgacgg
     2821 tccgggcgcg aaaaagccgg gaccgccggc aataaaaaac cccgccgggg cggggttttt
     2881 tactgagacc ggtcagtgat caccggtcac gggtcatctg cttcaggcgg gaacgggttc
     2941 gtgctccttg acgtccgctt ccgccggcgc ggccggtttg acgggttttg gcgatccgct
     3001 ctgcttcttc agttcttcct ccttgcgcat ctgctccatg cggcgtatcc agtagagctt
     3061 ctcttgataa tcatcgcaca tgacgggact cctctcgtga ataaggcgtg acaggtgaca
     3121 tgtgataggt gacaggtgac aggtgtcgcg cggagcgcgg ggtcttatca cttatcactt
     3181 atcacgcatt tgctcttgcc agcgggtctc ccttaccacg cgttggaatt gtgggttgat
     3241 gctacgcctc gcgcagacgg atatcaacgg ctcgccgtcc gaactgcaat cgcaatccgt
     3301 ggttcacctc cgataaccct gagataattg gggtgaaacg tccttcgccc tgtcccgcat
     3361 gagcgagccc atccagattc gcatgggagg ctacggcccc ccgagcacga cctgcagccg
     3421 tggactgaag gtcatgggcg accggctcgc ggcggagttc ggaagcgccg tggacgtcaa
     3481 gtacatctgg aacatcctgg atttcggcta caaggcgggc gacctgctgt ggatggccga
     3541 gcacggaatc ctgacgctca cttaccaatc gaccagctac ctcgccgacc gggtcccgga
     3601 gctggagttc gccgatcttc ccttcctgtt cgacggtttg ccggaggcga gggccgcgat
     3661 ggacggcaag ctcggcgcat ggttgagccg caagatcgag gagcgcatcc cgggataccg
     3721 cgtgctcggc tactttgaaa acggctaccg ccacgtttca aaccggctgc gcccggtgcg
     3781 cgcgcccgcg gacttgaagg gcatgcgaat ccgcctgatg cccggcaagg tccacgcccg
     3841 cagtttcgag ctgctgggcg cgataccgtt ttcattggac cttaagccgg ggctggaggc
     3901 gatcgtgtcc ggggccgtgg acgcgcagga gaatccgctc gccaacacgg tggactacgg
     3961 cgcccacaag gttcaccgct accacaccct gaccggtcac tgctatctct cgcgcggcat
     4021 ctacctgaac cgcgccgagt tcgagcgctg gccgagggag ctccaggagg ggatccagcg
     4081 agccgcgcgc gaggccatcg ccgcgcagcg tgagcttgcc gtcgaggaag agcagactgc
     4141 gcgcaagtcg attgtcattg cggggggcga agtactggag ctgaccccgg aggagcgcgc
     4201 cgcgtttgcc cgcctcgtca aaccgctgca cgacgaggcg cgcagccgct ttggcgaggc
     4261 ggtattcgcg ttcgcgaaaa ccccgtgacg agaaggtgac gagatgcggg tgacgagatg
     4321 cggatgacga ataagtgagc aatctatgtc tcccgaaaca aaaaaacccc gccgcaccgg
     4381 ggttttgtgc gggagctagg cagcagaaat tagcgcccgt caccattatt cgtcacctgt
     4441 aatcgtcacc cgtacctcgt cacttctttg gttgcgctga aaagtgaaaa ggtcaatcgg
     4501 tcgtgtagcg tgcgttggcg cggcgtccag agccgcgcat taccctgctg ccgctttcac
     4561 gccttttgtg atttcgttta atacgtaagg catgatgccg ccgtggcgga cgtagtccat
     4621 ctcggccggc gtatcgaggc gcagggtaac cgtgatgttc tcggtgtgcc cgtcggggcg
     4681 atgcacgacg agcatcagcg cctgccccgg cttcacgtca tcgtcaaggc cgatcaagtc
     4741 gaatacttca gagccattca agcccatggt cgcggcagtc gtgccgccgg gcaactggca
     4801 tggcagcacg cccatgccga ccagattgct gcgatgtatg cgctcgaagc tccccgcgac
     4861 gaccgcgcgc acgccgagaa gccgtgtgcc tttcgcggcc cagtcgcgcg cgctcccggt
     4921 gccgtattcc tcccctgcga cgacgatcag cggcacgcgt tccgattggt agcgcatcgc
     4981 cgcgtcatag atgctcatgc gctcgccgct cggttgatgc gccgtgacgc cgccttcgac
     5041 acccgggacc atgagattct tcagccgcac gttggcaaac gtgccgcgca tcatcacctc
     5101 gtggttcaga cggcgcgcac cgtagttgtt gaagtccgcc ggcgcgacgc cgagcttttg
     5161 cagatagagg ccggcgggcg atgtgctctt gatgcttccg atcgggctga tgtggtccgt
     5221 cgtgatcgag tggccgagta tcgcgagcgc gcgtgcgctc ttgatgtcgg tgagcgtcga
     5281 cagtctcagc tcctcgtcga ggaacggcgg ctgcctgatg tagctcgagt cgctctccca
     5341 tgggtacatt tcgccggtcg ccagcgggat ggttttccag agcgggttcc tgctcgcgat
     5401 gtcggcggcg taggtctcac gatagaaggc gggattggcc gcgacttcga gggcctgctg
     5461 cagctcttct gccgtcggcc atacatcgcg caagtgcacc ggctcgccct tatcggtgca
     5521 gccgagcggc tctttcgcga ggtcgatgtc gacacggccc gcgagcgcga acgcgacgac
     5581 gagcgggggg ctcgccagaa atgccgcgcg caccgccgga tggatgcgcg cttcgaagtt
     5641 gcggttgccc gacagcaccg cgcacgcgac cacgtccttc tcggtgatcg tcttctcgag
     5701 cccggcgtcg atcggtccgg acgcgcccac gcaggtgccg cagctgtagc ccgcgatcgc
     5761 gaagccgagc cgatcgaggt aacgctgcag ccccgtcgct gcgagatact tgctgacgac
     5821 ggtcgagccg ggagtgagcg acgtcttcac ccatggcttc gccttgagcc cgcgctcgac
     5881 cgctttcttg gcgacgagac ccgcagcgag catgacaccc gggttcgaag tgttggtgca
     5941 cgaggtgatc gcagcgatca cgacgtcacc gtggtccacc tcgccgttgg acctggagcg
     6001 caccgcggct ttgccataac cgccgcccgc gaccggctgc gtgagcacgc tttcgaatcg
     6061 gctcttgatc tcccgcaggg ggatgcggtc ctgcggccgc ttcggtcccg cgacgttcgg
     6121 ctcgacggtt cccagatcga gctgcaagac ccggctgtaa tcgacttcgc cttcgcgcgc
     6181 tgcgccgaaa cagccctgcg cgcgatagaa cgcctccact gcccagacgc gctcttcgga
     6241 tcttccggtc tgcaacagat aacggcaggt ctgcccgtcc acggggaaat agccgatcgt
     6301 cgcgccgtac tccaccgcca tgttggagag ggtcgcgcga tcgggcaacg tcaggttctt
     6361 cacgccttcg ccatagtatt cgacaaactg tccgacgact ttcgcctttc gcagcatttc
     6421 ggtgacatgc aggacaagat cggtcgccgt gacgccgtgc tcgagcgcgc cggtcacgtg
     6481 cacgccgacc acgtctggcg tcaggtagta caccggctgc ccgagcaccg ccgcttcagc
     6541 ctcgatgccg cccacgcccc atccgacgac gccgaggccc gcgatcatgc aagtgtgcga
     6601 atcggtcccg acgagcgtat cgggatagca aatgccgtct ttttcaaggt atcccggcgc
     6661 cagaaattcg aggttgagct gatgcagaat gccccagccc ggcggcatga gccgtatgcc
     6721 tttgtaagcc tgcatcgccc acttcacgaa acgaaagcgc tcgtggttgc gctggatctc
     6781 gagctccatg ttgcgcgcga gcgcgtcagg cgtgccgtgg taatcgaccg tgagcgtgtg
     6841 atcgagcgcc atgtccaccg ggaccttcgg ccccacaatc tcacccggat agcccatgcg
     6901 cctgaccgcc ccgcgtatgg ccgtgagatc gccgagcagc ggaatgcccg ccgcgcagtt
     6961 gagcaccacg cgcccgacga tgaacgggac ttcctcttcc cgcctgccgt tcggacgcca
     7021 gttcgccagc gcccgcacct ggcgctccgt cacgcgcttc ccgtcgcagt ttctgagcaa
     7081 ggattcgaga atgacgcgca gcgacacggg caggcgcgaa acgggtccga tgccgtgccg
     7141 ctcgagcgcg ggcaccgagt agtacgcata tttcctgctg ggcccgaggt cgatttcgcg
     7201 cctggcgata gtattgaagg aaggagtcat ggtttctgcc tggcgagcgc gcctgctcac
     7261 accgccgcga gatcgcgttg atcgagcctc atcgcgcggg tgagctcgcc tgccgaacgc
     7321 aggttctcga ccttgcgcac ggcatcgatc aactgtgacg cgcgggcatc ggacatgcag
     7381 tgccgcgcga gcagcttgaa tttcgcgatt cgctcttcgt cgctgcacgg atccgacggc
     7441 atgccgtggg gatccagcac ggcgctgtcg tgacgatctc catttgcaag ctcgagatgc
     7501 acgcgggccc cgtaatgctt gggataggct gcctccatct ccggatccac gacgatttcg
     7561 atgcgccgcg ccagcccgcg cctcgagggc tcgtcgagct gatcgccgaa gtacatcgcg
     7621 ggatcgcgtg gatcggccgt caccgcgagc gcggcgcaat atggcaggct gtattgcgca
     7681 cccatcgtgt cgggcggggc gacgttgccg ttgttgcggg cggcgtaact gcagacaccc
     7741 agccgcagct ttttcacctc ctccgccttg accgggcgcg cgccgcgcat ccccacgagc
     7801 tgttgcaccg tggcctgaat ccaggcgcag catggataga ctttcacata gacctgctct
     7861 accgcccagc gcctgccgag atccaacgcc agttgctcgg gccgggccga tttcccggca
     7921 aagacttcga gcagaccgaa aggcccgtcg agcgccgtcg gcggcgcggt gaaaccgcgc
     7981 gctgcgagtt gagccattcg tactccggct tccgccgcgc ggcctgcgtg catgcgcttc
     8041 atcatccccc cgcccgtacc cgccgcgaag ctcttggtac ccgatgaagt ggagcaggcg
     8101 agcccgaccg ccgtcgtaag ttgcgcccga ctcaggttca tcaccacgcc tgccgccatg
     8161 accgccccaa gcgggcccgc gagagcggtc tggtgatgac cgcgccgcgc ggcttcaacg
     8221 ccgagagcca aaccgacgcg gttgatcgct tcgtatcccg cgatgatccc caacaggaga
     8281 cgggagcccg gcgcatcgat cgcctccgca gcggccaatg cggcaggaac gacagtggcc
     8341 cccggatgca cgatcgcttc gtcgagcaaa tcgtccagct cgaaaccgtg agtggccgtg
     8401 ccgttgcata acgcggcggc cggcgcggca agcgtctcgt tacggcccag gatgctgctg
     8461 cgtccccggg aaccttccgc gagcatctcc tccgccatga ttcttgccca gggctgccga
     8521 gagccgaaca acgcgcagcc gagagagtcg agcaagcacc acttcgcata ttcgacgacg
     8581 cttccgggcg cggtttcgag agacagcgcc tgacaaaact ggatcagctc ctgcgccgcg
     8641 ccttgtttcg gtagggtttc tgctttcatt ttccattcct cgtgaagttc aacttgcttg
     8701 tgtggctgct gtcccgaatc cctcacccgc ctgcctgtat tcggcgcgcg gcgggctgaa
     8761 aatgtcgagg atcagcgcac ccgtcgcgcc cgtgcgcacg ccgtgtgata cgccgccggg
     8821 cgtgtaccag aaatcgcccg cggtcgcctc gacctcttct ccattctgaa ttcgcgtgca
     8881 tcggccttcc agcagcacgc cccactgctc ttcgggatgg ctgtggatcg tgccgctcga
     8941 attgggctcg acgcggacca cggagagcat gacgcgctcc ccgggaaaga tgcgcgcctc
     9001 tatcccttcg cccagtttgc gcgcgatgcc gcgcgcgtcg ccgtgcagct ggaaaaccca
     9061 tggtgcctcg ctcatatggc ctccgacttc gagattacac ttcggcgagc ttgatgccca
     9121 gccgcacggc ggcggtttgc gcctccgcca gggaaggcct ggcatcaacc agtttcaagc
     9181 gaccgccgcc ccggttcatg gcgtcgacga acgcgctttt gtcccgggct tcgatatgta
     9241 tgcgctgaac ttccggctcg gtgacgtgcc ggatctcgac ggcgatgcgg cgccgcgcga
     9301 tttccttgag cacgttgtgg taggccttga tgtccttcgt ggacgcgccg agaacggcgc
     9361 ccatcaggat gccgggaaca ttgatcgccc tgcgggcaaa aagatcggat gtcagctcga
     9421 tggtgatctt cttcacttcg ccgcccgcca gctcatgggc gatgcgcccc atgttggttg
     9481 gagatcccac ggcaatgctg cttccgccga ccaccgcttc cccgaagggg ctgatcagtg
     9541 ggttggcgct catggcgaac ttcctgatct cctcgcgcgc gcgggcaaga acgctccggc
     9601 ccgactcctg ttcggccgca aggacgcttc cggcgacaaa gccgtccact accggttccg
     9661 tcttgaagta ggggcgcatg taatccagat tgatcttcgt gatcaccggc gccgccgcgg
     9721 catgaatgtt ggcggccatc gcgagcatca cgtccacgtc gatgggcacg gcgatggagg
     9781 ttttcagcgc gagatgggcc gccaggttgc cgataaggac cgcgccgccg atgtgcgtcg
     9841 cgcagagccc cggaacaatg acccgcgggg tgcacggcac ggcaatcgtg ggagagatgg
     9901 cgagcacgat tgctttggcc acctggcgcg gcgacccttt gcgcatttca gccagtgccg
     9961 cagccgtgca cgccgccccg gcgccaaacc cttccatgtt gcagccggtg gttttcttgc
    10021 cctccttgaa gaaagttccg atcttgacca tcaaggcggc caaccgtccc acctgcaagc
    10081 gggaaagtct cgtttccagc agtgctttca gaagaccggt atacacgcac gaatccccgg
    10141 tccccgcaca gggttgcaag cctacttcgt gattgcccac ttcggtggcg atcgtgtaaa
    10201 cgacggcctt gtcgacaaac cggtcgccga tgactgcggt gccgcgtgtg cggcattcct
    10261 gtaattcccg gccgatggat cccagcagga aactgttgcc ccgaccgagc ccgatttcca
    10321 gcgccagcag gttgaacgcg aaggcgtcca gcaccgcccg gatcacctcg tcatgcgtca
    10381 tgtcgtgcgc cgccatcgct tcggcaatca cgatgtcgct catcctgacc ccgaggcttt
    10441 cggcgaggtc gacggtctcg cccagcgtga gctttttgcc agcggacgcg gttttcgcga
    10501 tttcggcaat cagccgcttg aaaaacaggt cttgtttcat ggcgaatact cctcggctcg
    10561 cagcgtgcgt gggcaacctg aatgtcgagt cattcaagcg tcgcgcccga ggtcctgacg
    10621 acttgcttcc agcggtcgta atcggccttg atcgtcttgc cgaagtcgtc gggcgagctt
    10681 ccgacgagga ccgtaccggt cggctcgaag cgatcgcgga attcctgcag caagagcacg
    10741 cgccgggttt cccttgcaag aatgtcgatg atcttccgat cggtgttcgc cggtgccagc
    10801 atgccgtacc agagatcgga ctccgcgcct ttcaggatcg gcagctcggc catcgcgggc
    10861 acgtcgggca gcgccttgtt gcgcgtggcg ctcgtcacgg cgatggctcg cagccgcttg
    10921 tccttgagca tcggggtgac cgtgaaaatg ctgatgaaca tgaagtccac ctcgccgctc
    10981 aagagtgcca tgatcgccgg cgcgccgccc ttgtagggca cgtgcgtgat gttgatcccg
    11041 gccatgttct tcagcatctc gcctgcgaga tgcgcgcccc caccggtacc ggacgagctg
    11101 taggtgagcc ctttgggagt cgatttggcg agcgcgatca gctccttcac gctcctggcg
    11161 ggcaccgtcg gatgcgtcgc gagcacgaac ggcagcgtcc cgatctggct cactggcgcg
    11221 aggtcccgca gcggatcgta cgagagcttc ttgtaaagat gcgggttgat gacgatcgcc
    11281 gcgttcgtgg cgacgagcac cgtgtacccg tcgggagcgg ccttggccac gacatcggca
    11341 ccgatattcc cattgccgcc tgcccggttc tccacgatga cttgctgccc gagcgtcttc
    11401 gtgaattccg gcgccatggc gcgcgcgacg atgtccgtgc cgccgcctgg cgcgaaattc
    11461 acgacgaacc ggatcggctt cgagggatag gtctgcgccg acgcgctgaa aacgccggtc
    11521 atcgcgatgg cgatcgccgt cgcggccagc ttacgtggtt ccgcaaacat gttttcctcc
    11581 gtaattttct atgtctccgc cagataccgc tgccgcgcga gctgcggcag cccgccgcat
    11641 tcgtagattc gctcgatcat cggcgggaac ctgggtatcg gaatggtccg cccggtcgcc
    11701 acgttcttga cgcagcccgc ccggtaatcg atctccagcg tttcgccgtc ctgcacgatg
    11761 ccggcgattc cgggtgcgac gtaaatcggc atcgggaggg cgatcgtgtt ccgcagaaaa
    11821 agccgcgagg ccgattcgac gacgatcgcc gcgacgccca cttcgcgcag cacggtcgcc
    11881 ggccggtgcg agccgcagcc gaagttgcgg ccgccgacga tcaagtcgcc cggcttcact
    11941 tccttcggga actccggacg gaacgattcg agcgtgtgct gcttcagctc ctccatcgtc
    12001 gagtacttgt agaacgggtt gatcgagtcc gtatcgaccg aatcgccgaa cttccagatg
    12061 cgcccgcgga tcacgcgatt cagatcaaat ctcatcgaac acctccttcg gatccacgat
    12121 ctttcccgcc accgccgccg cggtgacggt gagcggcccg gcgaggtaaa gctgcgcgtc
    12181 gaggctgccc ttgcgtcccc tgaagttgcg cgtcgtcgcc gtgatgcaca cttcgcccgc
    12241 tgacacgtat ccgaccttgg gctggcagat gccgcagccg ggcgtcacga cgtgcgcacc
    12301 ggactcgacg agctgctgga tgagcccggc cttcatacac tcgaggtaga cctgctggga
    12361 cgaaggcgag atcaagaagc gcacgccttt cgcgaccttg cggcctttca gcatgcgcgc
    12421 cgcgatctcg atgtcttcga agcggccgtt cgcgcacgag ccgatctgcg cctggtcgat
    12481 cttgataccg gcgacctcat tcaccggact gacattgccg aacgtgtgcg gcttcgcgac
    12541 cgcgaacggc atctcgtcaa cgtcgagcgt gatctcgttc tcatacaccg cgtccgcgtc
    12601 cgccgcgacc ggctcataag gccggtcggt tcgcgtgcgc aggtattcgc gcgttctgcc
    12661 gtcgaaggga aacacggcga acttcgcccc cacttcgacg ccgtgcgtgg aaagacacat
    12721 gcggctgtcg atgctcaact cgcgcacgag cggccccgtg aactcgatcg ccatgccttg
    12781 cgcgaagtcg tcaccgtgca gccccgcgag gtgcaggatg atgtccttcg cgatcgggta
    12841 gttgggttgc cggcccgcga gcaccacctt gatcgtgcgc ggcacctgaa accagagctc
    12901 cccgaagagc agcacgtgca gcatgtcggc gcgcgtgatg cccgtcgcgc cggcattgag
    12961 cgcgccataa gcgatcgtgt gcgagtcgtt gcccacgacg agctcgcccg ggcgcacgta
    13021 gccatagtcg ggcatcatct ggtgcgcgat gccgggctcg gcgtcgtgga aatgcttgat
    13081 gccgagccgc ttcgcttccc tgcgtatgag cacgttctcg tcggcgtacg actgggcgag
    13141 cgcgggctga tggtggtcca ccagcaggta gacgcgctcg gtgtcccaca cgcgcggaaa
    13201 gccttcttcg aaccccgcct ccacggccag ccgatggatg tccatgaact ggtagtggca
    13261 catggcgcca tcgatccgcg ccccgatgaa gtcgccggct ttcaccggcg cctgcgcgag
    13321 gttgtgccgg gagaggatct tttcggcgat cgtctgaccc atgctcacgc ctttattccg
    13381 ccttgagtcc gaggtccctg atgaccttac cccaacgcgc gtgctcctgc ttgagatatg
    13441 cggcgtactc ttctggagtg ttcgtgagaa cctcgaaacc cagcttgagg agctgctcct
    13501 tcactgcagg ctgcgccacg acctgcagga tctccttgtt cagcctgttc acgatctcac
    13561 gcggcgtgcc tgcggttgcc aatatcccga accaggtcag gacttcgaaa ccggggagcc
    13621 ccgattcggc gagcgtcgga aattcgggcg ccatcgccga gcgtttgagg ccggtcaccg
    13681 cgagaccgcg catctttccg gtgcggacgt gcggcagcag cgtggcgatg ctcgcgttga
    13741 aaagctgcac gtggccgccg aggatgtcga tgatcgcggc cccgtcaccc ttgtaatgga
    13801 ccggcaccat gtcgattttc gtgatgctct tgacgagctc catggacatg tggggagaag
    13861 aaccgatgcc gccagtcgca aaattgatct gccccggacg cgccttcgcg agcgcaacca
    13921 ggtccttgaa cgacctgacc ggcatggaag ggtgggtcac catgagcagc gggttatggg
    13981 cgacgcgcac gaccgtgacg aaatccttga tcgggtcgta tccgaccttt ggcaatagcg
    14041 ccggcgcgac cgcgtgggtc gtctggctgg cgagatacag cgtgtgaccg tcgggcgcgg
    14101 ctttcgccac gagttccgcg ccgatcgtgc cggtcgcgcc gggccggttc tcaacaagaa
    14161 actgctgtcc catgcgctcg gagaggccct gtcccaccat gcgcgcaacg agatccgacg
    14221 ggcctcccgc tacgaagccg acgatgatgc gcaccggccg cgacggatag gtttgcgcgt
    14281 gcgcgagcgc gacgcaggct aacgacagga gcaggaatcg cgtgaacaag ctcatgtttc
    14341 ctccgttgtc tttgccgtgt ttctgatccg ccttggcttt gctctcccga ttcacgccgc
    14401 cttggcgagc ttcgcggcca ttgcggcgct gcgcccggcg aggcggccga agacggcgcc
    14461 gctcatgagc ccggtcgagc cgggatagtt gaaatagaac agcccgccga ccatttcgcc
    14521 cgcggcataa agcccggcga tcggttgatg gttggtgtcg atcacctgcg cgtcggtcgt
    14581 gactttcaga ccgccgaaag tgaaagtgat gccgcaggtg acggcataag cctcgaacgg
    14641 cgggttctcg agcggaatcg cccaattcga gcgcggcacg cacaatccca ccgccgcgcg
    14701 cccgtccttg acgttcgggt tgaatggcac gtcgcgcttc accgccgcgt tgaactcgcg
    14761 aatcgtcttg agcaactgct ccttgttcac atcctccagc ttgtcagcaa gctcttcgag
    14821 gctgtccgcc ttgactttcg tcacgtgttt ggagcggtat tcgggccgca gcaggtgcgt
    14881 gaccgatgaa tcgaagatct gccaggcgaa ctggcccggc tgctcgagga tgatgcggcc
    14941 gtacttggca tacgtgtagt tgcggatgtc ggcgccttcg tcgaggaagc gcctgccgtc
    15001 ggcgttgacc atgatcgaga acgggtagct gtcccgctcg taatcgtcga cggtcgagaa
    15061 atcgccgaag tcggatgcgt agcgttccca gccgacggca tggcaacccg accaatgacc
    15121 gcagggctgc gcgcctatcc tgagcgccat ggcgaggccg tcgcccgtgt tgaatctggt
    15181 accgcgcacc ttggccaggt cccagccctt gccgagatag cggctgcgcc attcggcgtt
    15241 cgcctcgaaa ccgccgcagg cgaggatcac gctcctcgcg cgaatgtcgc gcgtggcgcc
    15301 cttcagacgc gcgaccacgc cttgcacgcc ggcatccgag tgcaggagat ccctcaccca
    15361 ggcgttgtag tgcacctgaa caccggcctt ctcggcggct ttgtagagcg tatcgatgag
    15421 gccgcgcccg ccgcctgacg ccgcgagcgt cgcgccgccc cagaacgtgt acttgccgcc
    15481 gatcttgaac gattgccggc cgaactgagg aatccagcgc acgcccttgt ccttcagcca
    15541 gtgcatggtc gggttgctcc cgcgcacaag gatttcgcac aggtccggat cggtgcggtt
    15601 ttgcgtaatc cgcgccatgt cgtcgaagaa cttctcttcg gtgtaggtgc cgaagtcgct
    15661 cttcatttcc tcttcgctca agtcgggcga aagcgcgcgg atgtcatcgg ggccgttgta
    15721 aacacagcgc agcaggccct cggtgaagct gctgttgccg ccgcgctctt cctcgggcgc
    15781 gcgctcgagc aggagcacct tcgcgtcttc gtcactcgcc gagatcgccg cgcacagcgc
    15841 cgcgttgccg ccgcccacta cgatcacgtc ataccgggtt tccgtccttt ccattacatg
    15901 ctccgcttcc aatcaatcaa cccgctcgta gagcagagtg ttcttcttga gcgccgcttc
    15961 gtcgatgctg atgcccagcc ccgggccgcg cggcacgtcg acgtgtccgt cgcgcgcgac
    16021 gaaatcggtt tccggaacca ggggatcctt gccgaccgcg ttgagcccga atacgaattc
    16081 ggccgccccc atcatgcgcg aggccggggt gctcgcgtag taatgagcgc cggctgccgc
    16141 ttcgagttgc gattgcacgg caagaccgcc gcagttcagc tgaatgttcg ccgcctcggc
    16201 gatcgcggcg atcttcttcg ccggcgtgaa tccgccgagc ttgtagagct tgatgcagta
    16261 ggcgtcgacc gcgcgcgcct cggcgagcgc atacgcatcc tgtatcgtga agagcgcctc
    16321 gtccgccatc accggcacgc cgtccgccgc gcgccggatt tcggccatgc ctttgagatc
    16381 gcggcgcgcg atcggctgtt cgatatagcc gagatccatc ttcttaagct cgcggatcgc
    16441 cgacagcgcg tcggccaggg tccagccggt attcggatcc accccgatca tcacgccgtc
    16501 gcccacggcc ttgcgcacgg cctgaaagtt cctcacgtcg tgacgccacc cgcggcggtc
    16561 ggccatcttc aggcacagca cccggatccc gaactcgtcg acggcgcgct tcgcctccgc
    16621 aaccatggtc gcagtttcct ccgccatgct caccgaccac tcgagcggga tgcgcggctg
    16681 gcacaacccg cccaggaggt tgtagagcgg ctggttggtc gccttgccca tcgcgtcacg
    16741 taccgcgatg tcgagtacgg cgcgagccgc cgggttgttg gcaaggcgcg cgtcgaatgc
    16801 ctcgttgatc gcctcgatgt cgaagatcga cttgccgata agcgccgggc cataaatctc
    16861 cttgacggcg gcgaccacgc tgtgcgtcgt gtcggcgacg aagtgtcccg gactgatggg
    16921 ccggatctgc gcgcagccca ccacgccgtc ggcgtggatt ttcacgagca ccggcttgga
    16981 gagcagccgc tccgccggcc ccgtgtcata gctgccgctc gagaagatgc gctgcacccg
    17041 caccggcaag ccggtcacat ggatttcgat acgatcgatc actgccatgt tcgcactggt
    17101 ttggaatcgc gctgatcaga cgcgcgctgc cgcgagcacc atatcggccc ccttctcgcc
    17161 gatcgcgatc gcggccgcgt tggtgttcga cgaggggatc gtcgggcaga tcgacgtgtc
    17221 caccacgcgc aggctcgcga tcccgcgcac gcgaagctca ggatcgacca cggccgtatc
    17281 atccacgccc atcctgcacg tgccgaccat gtgccagctc gtctgcacgg tctcccgcac
    17341 gtagtcgagc agcgccgggt tgtcgttcgt ttcggggccg ggccgcgttt cgcgcacgat
    17401 gagcgatttc agtgccggct gccgcgcgac cttgcgtgcg acacgaatcc cgtctaggaa
    17461 gagctgcgca tcggcctcgt gcgagagata gcgcgggtcc atcgcggcct ggtcgtgcgg
    17521 gtccctggag cgaatgtgca gcgttccgac cgaacgcggc tggagcggcg tgatgccgaa
    17581 ggtaaagccg ggaaacgggt cgaggccgta cgccttggtg cgggcgtagc ggtcctttcc
    17641 ggacaccggc agcaggcgca gcacgaggtc gggctgcttg acctccttcg agctgcgaaa
    17701 gttggtctgc g
//
LOCUS       DBNGWZ010000202         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2664710, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000202 DBNGWZ010000000
VERSION     DBNGWZ010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2664710"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="AC16CX_05915"
     CDS             complement(<1..2054)
                     /locus_tag="AC16CX_05915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012084.1"
                     /translation="MRTAVRHSRRDFLKTAGAIVVGFSWSVPGVLAQQASPARLPGSL
                     NTNRMLDGWLRINPNGTVTIFTGKCELGQGILTALAQIVSDELDVAYERIEMVSADTA
                     RTPNEGMTAGSQSVENSGTALRYACAEARQILLGLAAAKLGTTADRLTVSDGTVSSGG
                     GKTTYAELAREANLKREATAQVKPKPSSRYKMIGKNVQRRDIPSKVTGGPSYVQDMRL
                     PGMLFGRVVRPPSFRAQLASFDEAAIKAMPGVVAVVRDGNFLGVAAEREEQAIKAARA
                     LRASARWNETPDLPPPAPELFEHLQQMRSQDTVVNQKISATPAGGKVTSHEATYTRPY
                     QAHASMGPSCAVAQVRDGKYLVWTHSQGVVPLRSELNKVLGVPEANITCTHVEGAGCY
                     GHNGADDVALDAILVARATGGRPVKLQWMRDDEFSWEPYGSAMVMKMRARLDDSGSIV
                     DWRHEVWSHPHSTRPTSNPGGNLLAGWHIAKPVAQAPSPNIPQPAGGADRNAVPTYDF
                     PSQQIVLHYITDLPIRTSALRTLGAYANVFALESFMDELAAAAGADPVEFRLRHMKDP
                     RARAVIETCAQKAGWKPGQKGDGTRGRGFAFSRYKNLACYVAVVADVTVDRRTGKARV
                     TKAVSAVDAGLIVNPDGLINQIEGGIIQSASWTLKEHVGFDRQRITTRSWADYPILTF
                     PEV"
     gene            complement(2051..>2307)
                     /locus_tag="AC16CX_05920"
     CDS             complement(2051..>2307)
                     /locus_tag="AC16CX_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012085.1"
                     /translation="SVTTLEGLGSKDRLHPLQKAFIEEQAAQCGYCTNGMIMAAKALL
                     DRNPKPTEAQIRKALAANLCRCGTHNRVVRAVQRAAKEAA"
BASE COUNT          322 a          765 c          792 g          428 t
ORIGIN      
        1 acctcgggga aggtgaggat cgggtagtcg gcccacgagc gcgtggtaat gcgctgccgg
       61 tcgaacccga cgtgctcctt cagcgtccag ctcgccgact ggatgatccc gccctcgatc
      121 tggttgatca gaccgtctgg attgacgatg aggcccgcat ccacggccga caccgctttc
      181 gtcacgcgtg ccttgccggt gcggcggtcc acggtcacat cggcgacgac cgctacgtag
      241 caggcgaggt tcttgtagcg ggagaacgca aacccgcggc cgcgcgtgcc gtcgcctttc
      301 tggcccggct tccagcccgc tttctgcgcg caggtttcga tgaccgctcg cgcacgggga
      361 tccttcatgt gccggagccg gaactcgacc gggtcggcgc cggcggcggc cgccagctcg
      421 tccatgaacg attcgagcgc gaatacgttg gcgtaggcgc ccagcgtgcg caaggccgac
      481 gtgcggatcg gcaggtcggt gatgtagtgg agcacgatct gctggctcgg gaaatcatag
      541 gtcggcaccg cgttgcggtc ggcgccgccc gcaggctgcg ggatgttggg agagggcgcc
      601 tgggcaaccg gtttcgcgat gtgccagccc gccagcagat tgccgccggg attgctggtg
      661 ggacgcgtcg agtgcggatg gctccacacc tcgtgccgcc agtccacgat gctcccggaa
      721 tcgtcgagcc gcgcgcgcat cttcatcacc atcgccgagc cgtacggctc ccacgagaat
      781 tcatcgtcgc gcatccattg cagcttcacc gggcggccgc cggtcgcgcg cgccaccagt
      841 atcgcgtcga gcgccacgtc gtcggcgccg ttgtgcccgt agcagcccgc gccctcgacg
      901 tgggtgcagg tgatgtttgc ctcgggcacg cccagcacct tgttgagctc gctccgcagc
      961 gggaccactc cctggctgtg cgtccacacc aggtacttgc cgtccctcac ctgcgccacc
     1021 gcgcacgagg gccccatcga ggcgtgtgcc tggtaggggc gggtgtaggt cgcttcgtgg
     1081 ctggtgacct ttcctccggc gggggtcgcg gaaattttct ggttgaccac cgtatcctgc
     1141 gagcgcattt gctgcaggtg ctcgaacagt tccggcgcag gcggcggcag atccggagtc
     1201 tcgttccatc tcgcggaggc gcgcagcgcc cgcgccgcct tgatcgcctg ctcctcgcgt
     1261 tccgcggcga cgccgaggaa gttgccgtcg cgcaccacgg cgaccacgcc gggcatggcc
     1321 ttgatcgcag cttcgtcgaa cgaggcgagc tgggcacgga aggagggcgg acgcaccacg
     1381 cggccgaaca acataccggg cagccgcata tcctggacat agctcggtcc accggtgact
     1441 ttggacggga tgtcgcggcg ctggacgttc tttccgatca tcttgtaccg cgacgacggc
     1501 ttgggtttga cctgggcggt ggcctcgcgc ttgaggttgg cttcgcgcgc cagctccgcg
     1561 taggtggttt ttccgccgcc gctcgaaacc gtgccgtccg aaaccgtcag ccggtcggcc
     1621 gtcgttccga gcttggccgc ggcgagcccg agcaggatct gccgcgcttc cgcgcacgcg
     1681 tagcgcagcg ccgtgccgct gttttccacc gactggctac cggcggtcat gccctcattg
     1741 ggcgtgcgcg cggtgtcggc cgagaccatc tcgatgcgct cgtaggcgac gtcgagttcg
     1801 tcggacacga tctgcgcgag cgcggtcagt atgccctggc ccagctcgca cttgccggtg
     1861 aaaatggtga cggtgccgtt gggattgatg cggagccaac cgtcgagcat gcggttggta
     1921 ttgaggctgc cgggcagccg cgccggcgag gcctgctgcg cgagcacacc gggaacgctc
     1981 cagctgaagc cgacgacgat cgcgccggct gtcttgagaa agtcgcggcg cgaatgacgg
     2041 acagcggttc tcatgcggcc tccttggcgg cgcgttgtac cgcgcgcaca acgcggttgt
     2101 gagtgccgca gcggcagaga ttcgccgcca gggccttcct gatctgcgct tcggtcggtt
     2161 tcgggttcct gtcgagcagc gccttggcgg ccatgatcat gccgttggtg cagtagccgc
     2221 actgcgccgc ctgttcctcg atgaacgctt tttgcaacgg gtggagcctg tcttttgacc
     2281 ccaggccttc gagcgtggtg acgctct
//
LOCUS       DBNGWZ010000203        17648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_398506, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000203 DBNGWZ010000000
VERSION     DBNGWZ010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_398506"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16CX_05925"
     CDS             <1..341
                     /locus_tag="AC16CX_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012086.1"
                     /translation="LTNQQLAELLIGIARAQQAMIDAIESQRAGYKQTHLGPALTTAA
                     KIRNTGHVATLMDFPARVLLAHQGRNPPVLAQVLRDLEALLSGQPSAPAAAAAAPSAP
                     AADAPSLDMT"
     gene            complement(427..1194)
                     /locus_tag="AC16CX_05930"
     CDS             complement(427..1194)
                     /locus_tag="AC16CX_05930"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR3012087.1"
                     /translation="MNDEQLLRYSRHILLPQIGIEGQEKLLAARALVIGAGGLGSPVA
                     LYLASAGVGGITLCDNEEVDLTNLQRQIVHRTASIGRKKVDSARATLDAINPEVKVAA
                     LAERVDEKRLEALAAQHDVVVDGSDNFATRHAANRACVRLKKPLVSGAAVRFDGQVAV
                     FDLRAPDSPCYACLFPEHGESEDVRCAVMGVFAPVTGIIGSIQAAEALKLIAGAGETL
                     AGRLLLLDALAMQVRTIRLQKDPACPVCAAAARSAEL"
     gene            complement(1336..2751)
                     /locus_tag="AC16CX_05935"
     CDS             complement(1336..2751)
                     /locus_tag="AC16CX_05935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3012088.1"
                     /translation="MRNKLHQIGLLVIGVLIGVAVSLNFSAVAQKAVTTAPLPIEELR
                     AFTEVFGRVKSDYVEPVDDKKLITEAINGMLTGLDPHSAYLDQEAFKELQVGTQGEFG
                     GLGIEVGMEDGFVKVVAPIDDTPASRAGIKPGDLIVKLDETSVKGMTLNDAVKRMRGK
                     PNTQITLTIVRKGEPKPIVVTLTRSVIKIQSVKSKILEPGYAYFRVSQFQEHTGETLA
                     RAIERHFKENQGPMKGIVLDLRNDPGGLLNGAVAVSAAFLPAGSLVVYTDGRTEDSKM
                     KLNANPEHYLRGRMKEDYLRKLPAEAKTVPMVVLVNGGSASASEIVAGALQDHKRAVV
                     MGQQTFGKGSVQTILPLGNSTAIKLTTARYYTPNGRSIQARGIMPDIPLDDGASAASL
                     KLRESDLTKHLIDNGKEEEKAKATAEAARQYNFTPEPRPKDIDEKLLRPEPGEVVSKH
                     DYELNQAIAFLKSRITTRVAN"
     gene            complement(2851..4029)
                     /locus_tag="AC16CX_05940"
     CDS             complement(2851..4029)
                     /locus_tag="AC16CX_05940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3012089.1"
                     /translation="MRARSAAVLVLLLLASPGAEPAGASAKERELRELRGRIEALQKS
                     LADAEETKSEAADALRESERAISEANRALRELGAQSREINQRLAELDAESRSREQALK
                     AQQTLLARLLYHHYLGGQSEPLKLLLNREDPNEIARRLHYFGHISRARARLIAETRES
                     LVRLRQLSEEAGRKAEELAAVTAESQRLRARLEREKRARNQVLARISRDIQRQRREIG
                     TLKRNESRLARLVERLSKLVARAKPAPRLKNERVPQASSGDSPFLELKGRLSLPVRGE
                     LANRFGSPRADGGVIWKGLFIVARAGEEVRAIADGRVVFADWLRGFGNLLIVDHGDAY
                     MSLYGNNEALYKRVGDPISAGEPVATVGASGGNVDSGLYFELRHQGRPLDPLDWVNIR
                     "
     gene            complement(4026..5561)
                     /gene="gpmI"
                     /locus_tag="AC16CX_05945"
     CDS             complement(4026..5561)
                     /gene="gpmI"
                     /locus_tag="AC16CX_05945"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3012090.1"
                     /translation="MKVTPVLLIILDGFGHREECEHNAICQARKPHWNFLWQTCPHTI
                     IDASEKWVGLPKDQMGNSEVGHMNIGAGRIVYQEYTRIENAIETGEFFRNPALLEAVE
                     TARGAGRALHILGLVSPGGVHSHELQIHAMLELAARAGVKEVQVHAFLDGRDTPPRSA
                     EASLAALQEKCGELKAGRIASICGRYYAMDRDGRWERVQPAYDLITQGRADYRAGSAV
                     EGLHAAYARGETDEFVKATSIGSAGARMNDGDAVVFMNFRADRARQLTRALTDPGFKE
                     FARKHVPKLGYYCTLTSYGEDFARIPAAFQPQSVAGGFGEYISAKGLKQLRIAETEKY
                     AHVTYFFNGGVEKPYPGEERVLVPSPKVATYDLKPEMSAYEVTDRLVEAIKSRKYGAI
                     VCNFANGDMVGHTGNLAAATRAIEVLDECIGRAVAAMREIGGEVLITADHGNAETMLD
                     PVTKQAHTAHTLNLVPLLYVGRKANIADRGALQDVAPTLLAIMGLPQPPQMTGKSLIT
                     FSS"
     gene            5630..7378
                     /locus_tag="AC16CX_05950"
     CDS             5630..7378
                     /locus_tag="AC16CX_05950"
                     /inference="COORDINATES: protein motif:HMM:NF023274.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglL family O-oligosaccharyltransferase"
                     /protein_id="HIR3012091.1"
                     /translation="MLIGLAWTLPFLQPYHRFPLTAFYSEWLAFALGLAAALVLLCKK
                     PWREAALPAVAIAPIGLIVVLGLHVALGRVPYPEQALTAALYLLWAVLLVVLGHALRR
                     VFALTIIATTLAWFMLAGGVLSALIGLLQHHQLSTPFDFLVGRKIALQVYGNLGQPNH
                     YAAYLFCALVSTVYLYSRRCLNGAMAAGPTALFLLVLTLAASRSSWLYLGALTTLAWL
                     LHRYRRDAESRRLAVIVLWLLPGFIVAQWVATLPFMMPSGARLVTSAERLFQVAGGIE
                     PRLQLWREAWGLFLGAPVLGAGFGQFAWHHFLHQAATGATAATGVFSHAHNIVMQLMA
                     ETGIVGALIIVGAVLLWLADLRRIRFGLDWWWLLALLAIIGIHSLLEYPLWYSYFLGV
                     AALLLGFGAERVFAVRFAGAARVVAGLGIIAGWLNLALALEPYREFERLVFTPESRAV
                     RRMDETTFAKATVALYREPLLVPYVDLAIAYGVAVNDDRLAEKVALTARAARFAPVPV
                     VVYRHALLLALAGERKAALAQLERSLRVYPEEAGDIISQLEELARRRPDEFTPLLHLA
                     VARGAVLRARGARVSR"
     gene            7380..7502
                     /locus_tag="AC16CX_05955"
     CDS             7380..7502
                     /locus_tag="AC16CX_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012092.1"
                     /translation="MPTVIKIMGKHAEWHPAGATPLIKLATAKLAERRTSLGGR"
     gene            7529..7963
                     /locus_tag="AC16CX_05960"
     CDS             7529..7963
                     /locus_tag="AC16CX_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3012093.1"
                     /translation="MEQFLVFLQKSPFNMLLFGLALASGGMLLWPLVSRPFRQANEVG
                     AFEAVQLINRRDALVLDIRDTGDYAAGHITNARHIPEAQLPDRMKELDKHKSRPVIVS
                     CRTGSRAPAVSGMLRKRGFSEAFALRGGIAAWQQASLPLEKS"
     gene            7964..8224
                     /gene="grxC"
                     /locus_tag="AC16CX_05965"
     CDS             7964..8224
                     /gene="grxC"
                     /locus_tag="AC16CX_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HIR3012094.1"
                     /translation="MAKVTMYSTGICPFCQRAERLLRAKGVSAIDKIRVDLEPERRVE
                     MMEKTGRRTVPQIYIGETHVGGYDELAALERAGQLDGLLQAV"
     gene            8252..8716
                     /gene="secB"
                     /locus_tag="AC16CX_05970"
     CDS             8252..8716
                     /gene="secB"
                     /locus_tag="AC16CX_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR3012095.1"
                     /translation="MSEQQPVFTIEKVYLKDLSLEIPNAPQVFTEREAPKVDINLHNE
                     ARSLDNGVYEIVLTATVTAKLQDKTVFLVEVAQAGIFQIRNVQQQELDLLINVACPTT
                     LLPYARETVATILNRAGFPPVLLPHVGFETLYQQRLQEQQQQQQQAGVPPAP"
     gene            8736..9221
                     /locus_tag="AC16CX_05975"
     CDS             8736..9221
                     /locus_tag="AC16CX_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3012096.1"
                     /translation="MISDWTKNAYLRGTHHLSALIVAFFFGTGAGGAAAAADFRSVAE
                     NAAVLYDAPSAKAKKLYVVSHGYPFEIVVVVEGWSKVRDANGELTWIESKHLADRRTV
                     LVKAPLAQIREAADDGAVVVFQAQQNVLLELLEVAGGWLRVRHRDGQSGFVRAAQVWG
                     A"
     gene            9322..10308
                     /locus_tag="AC16CX_05980"
     CDS             9322..10308
                     /locus_tag="AC16CX_05980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3012097.1"
                     /translation="MKIAVLGAGAWGTAISINLCARHEVRLWARDAGLIEALRVDRSN
                     RRYLPGFALPSALALDKDLGATLRGAELILIAVITNGLRDTLQRIRAAGFSAPLVWLC
                     KGFESGSARMPHQVCAEELAPGMPRAVLSGPSFAEEVARGLPAALTLASSDGGFARSA
                     ARALHGARLRIYLSEDVVGVEAAGAVKNVMAIAAGVSDGLELGLSARAALITRGLAEM
                     TRLGVALGGRAETFMGLAGAGDLILTCTGDLSRNRKVGLALARGTPLAEVLAGLGHTA
                     EGVLTARAVAGLAGGLGVEMPITRAVCRVLDDPRQARAAVQELLAREQKTEY"
     gene            complement(10406..10873)
                     /locus_tag="AC16CX_05985"
     CDS             complement(10406..10873)
                     /locus_tag="AC16CX_05985"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR3012098.1"
                     /translation="MFEIVLFEPEIPPNAGNVIRLCANTGCRMHLVHPLGFSLRDKQL
                     QRAGLDYHELADIVRHEDWRACRSVLAGRRLFAVTTRGERRHDEPRYREDDVFVFGPE
                     TRGLPGEILAEFPPEHRIRVPMREASRSLNLSNAVAIVVYEAWRQLGFRGSRP"
     gene            10971..12689
                     /locus_tag="AC16CX_05990"
     CDS             10971..12689
                     /locus_tag="AC16CX_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3012099.1"
                     /translation="MGRPEKVRLGEILVQQRLLTEEQLKSALEEQKKSGRRIGRVFIE
                     KGFLTEEQISEALARQLNVPYISLKSYNRREDIKREVALKLPETQARRYRALVLEDAG
                     RTYKVGMVDPTDLMAYDEIARILERDIEIAVVTETELLRAIDRVYRRTEQITGLAQEL
                     GDDLGETTAVDFGAVALTPGLEEAPVVRLLQTVFEDAVQARASDIHIEPQEKKLQIRF
                     RIDGVLHLQTESDTKIATAVVLRLKLMSGLDISEKRLPQDGRFNVKVRNAQIDVRLST
                     MPTQHGESVVMRLLNQATGMLGLDNIGMPADMLAKLREVIHRPSGMVLVTGPTGSGKT
                     TTLYAALSEINTTERKIITVEDPVEYRLHGINQVQVNEKIELTFDRVLRSALRQDPDV
                     ILVGEMRDKPTVETGLRAAMTGHMVFSTLHTNDAVSTPIRLLDMGAPRYMVALSLQLV
                     LAQRLVRVICESCAQDYTLLPNEHEWLRHELGDQVDQHRYKRGKGCSQCNATGYLGRS
                     GVYEMLEMTIPVVEAANSDDPALFVRAARKQMSGNTLRRHAAQLVVAGRTTVEEAIHV
                     SSQSDD"
     gene            12745..13974
                     /locus_tag="AC16CX_05995"
     CDS             12745..13974
                     /locus_tag="AC16CX_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3012100.1"
                     /translation="MPHFAYKGRDDRGQPVQGVLEGADSGTVAGQLFNIGITPLEITP
                     SSAPRQSGPGLFQRMGEQAITHTDVLMFSRQMYTLLKAGVPIMGALKGLQESTDNKTF
                     GRVIQEIRESLDSGRELSASLARHPTAFNSFFVNMVRVGEHTGKLEEVFLRLFHHMEF
                     EQFIREQVKSAIRYPLFVVATMAIAIVIINLFVIPAFAKVYAGFKAELPPLTKILIAF
                     SDFMLTFWPALLAVLLVLVFGLRFYVNTVQGQLAWDRFKLRIPIAGRIVLKATLARFA
                     RSFALAIESGVPVVQALTIVANTVENEYVSLKIAAMRESVERGESVLRTAVATGIFTP
                     IVLQMVAVGEESGALDDLMKEIADMYQREVEYDLKNLAAQIEPILIVALGVLVLILAL
                     GVFLPIWDLGTAAFSKK"
     gene            14006..14632
                     /locus_tag="AC16CX_06000"
     CDS             14006..14632
                     /locus_tag="AC16CX_06000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3012101.1"
                     /translation="MFKVQGSAFEVHGPGTRNSKPRGGGFTLFELMVVIVIFSMLVAV
                     LLNRLGYYLELAEKAAMESTLRAIKTGLQTRLAELIITRRQAETSMLEVEDPTRWLEQ
                     KPPNYAGAYREPAEPGHWYFDERQRQLVYVVNSGQRLEIEGAPGAKELRFRVLLLVDR
                     HEAGGKRVQSVSGVTLVPVRAYRWSGAGHGRILARVLHQYQRNADVDS"
     gene            14691..15122
                     /locus_tag="AC16CX_06005"
     CDS             14691..15122
                     /locus_tag="AC16CX_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3012102.1"
                     /translation="MVIIILGILAAVALPRFANLQTQARIAKLSGALGAMQGAAALAH
                     AACLTSTPQCSGTQPMEGVNITMINAYPTANAAGIIAAAGLNVGPTSPDGYDVAGGGP
                     AAGDVLTVMALGNNPPTCSFTYTAPAAGQAPVFSTLTTAGC"
     gene            15219..15668
                     /locus_tag="AC16CX_06010"
     CDS             15219..15668
                     /locus_tag="AC16CX_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3012103.1"
                     /translation="MTAYQRGFTLVELIVVIVILGILAATALPRFINVQTDARIAAVQ
                     GIAGGLRSAVSVVQARWQVSGGSGVTSVNMADGTAVAVAGTTGIPTNAAGGIGAAMRC
                     ESATACQGVTIDLTVSPSTWRPSGGSATCQASYDPTAGDVVTTTGGC"
     gene            15811..16266
                     /locus_tag="AC16CX_06015"
     CDS             15811..16266
                     /locus_tag="AC16CX_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3012104.1"
                     /translation="MRQPQEGGFTLVELVVVIGVVGLLAAIITPRLIGTDAFASRGFY
                     DKAQAVVRYANKTAIAWRRPIFVCVTATSISAGGAAGCGSPLVNPATGGALTATAPSG
                     VTLSPASFSFDSAGRPNPNSTITIVVTSAIAGDPARQIVVEAETGYVHP"
     gene            16253..16912
                     /locus_tag="AC16CX_06020"
     CDS             16253..16912
                     /locus_tag="AC16CX_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3012105.1"
                     /translation="MSIPESSSEFRVPSSEFKEQAKPRSPINWKLKTGNSRRRKSLGF
                     SLVEQIVFIVVVGVAVAGVLLALNVVTRGSVDPVVQKQALAIAEAVLEEVQLQPFTYC
                     DPDDANAATALNAAGCATTAEALGPEPGESRLSATAPFDNVNDYHGFSMSGGITDIAG
                     DSISGLGSYSVSVSVAGQALGGIASSDALLITVTVTHPAITPGLVLHGYRVRYAPNAL
                     P"
     gene            16926..>17648
                     /locus_tag="AC16CX_06025"
     CDS             16926..>17648
                     /locus_tag="AC16CX_06025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR3012106.1"
                     /translation="MQTVPNHGSRIANLGIGCARGVTLVEMIVVIAITGIIAAGVALF
                     IRRPVEGYIDAARRAELSDIADTALRRITRDLRTALPNSIRITESPPASGVFYLEYLQ
                     TSGGGRYRAEVDSGGMGDILAFTAADSTFDVIGTMPTLAAGDLIVIYNLNADPAIPTA
                     NAYSGDNRAAYSSNTATTITLAAAHQFPFASPGKRFHAVQYPVTYECNPGTGALRRYW
                     NYAIAAAQSTPPVTPNNALLATS"
BASE COUNT         3035 a         5583 c         5777 g         3253 t
ORIGIN      
        1 acctgacgaa ccaacaactc gcggaactgc tgatcggcat cgcgcgcgcg cagcaggcga
       61 tgatagacgc catcgaaagc cagagggcgg gctacaagca gacgcacctg gggccggccc
      121 tcacgactgc ggcgaagatc cgcaacaccg gccacgtggc aacgctgatg gacttccccg
      181 cgcgggtgct gctcgcgcac caggggcgca atccgcccgt cctggcgcag gtcttgcgcg
      241 acctcgaagc gttgttgagc ggccagccgt ccgcgcccgc cgcggcggca gcggccccct
      301 ccgcgcctgc cgcagacgcg ccatcgctcg acatgacctg aaagtagtgt ttagtgttga
      361 gtgcttagtg ttgagtggag ggctgccttc acaggtcttg tatactggat acttaaaact
      421 caacactcat aactcagcac tccgcgcagc ggctgcgcac actgggcagg ccggatcctt
      481 ctgcaggcgg atcgtgcgca cctgcatcgc gagcgcgtcg agtagcaaca gccgcccggc
      541 gagcgtttcg cccgccccgg cgatgagttt caaggcctcc gccgcctgga tcgagccgat
      601 gatgcccgtc accggcgcaa acacccccat caccgcgcag cgcacgtcct cgctttcccc
      661 gtgctccggg aacaggcagg cgtagcacgg ggaatcgggc gcgcgcaggt cgaacaccgc
      721 aacctggccg tcgaagcgca ccgcggcgcc tgacacgagc ggcttcttca gccgcacgca
      781 cgcgcggttc gccgcatgac gggtggcgaa attgtcgctg ccgtccacca cgacgtcgtg
      841 ctgtgccgcg agcgcttcga ggcgcttttc gtccacgcgc tccgcgagcg cggcgacttt
      901 gacctcggga ttgatcgcgt cgagcgtggc gcgcgccgag tcgacctttt tcctgccgat
      961 cgaagcagtg cggtgcacga tttgccgctg caggttggtg aggtctacct cctcgttgtc
     1021 gcagagggtg atgccgccca ccccggcgga agccaggtag agcgcgacgg gcgagccgag
     1081 cccgcccgcc ccgatcacca gtgcgcgcgc ggcgagcagc ttctcctgtc cctcgatgcc
     1141 gatctgcggc agcagaatat ggcggctgta gcggagcaat tgctcgtcgt tcactggagc
     1201 gtgaaggggg aatgaggaag ggagaaggga aaaggcatgc ttccgcgccg cccgagtgcg
     1261 cgtgccgagg cagtgtacag caaacgcctg gctgacgccg ggcgttgctg aaggctgatt
     1321 gctgactgct gaaggctaat tcgcaacccg ggtggtaatg cggcttttca gaaacgcaat
     1381 ggcctggttc aattcgtagt cgtgtttcga cacgacttcg ccgggctccg gcctgagcag
     1441 cttctcatcg atgtccttcg gccgcggctc gggcgtgaag ttgtattgcc tggcggcctc
     1501 cgccgtcgcc ttggccttct cttcctcctt gccgttgtcg atgagatgct tggtgaggtc
     1561 ggactcgcgc agcttcaggc tcgcggcgct cgcgccgtca tcgagcggaa tgtccggcat
     1621 gatgccgcgg gcctggatcg agcgtccgtt gggcgtgtag taacgcgctg tggtgagctt
     1681 gatcgcggtg ctattgccga gcggcagtat ggtctgcacc gagcccttgc cgaaggtctg
     1741 ctggcccatg accaccgcgc gcttgtggtc ctgcagcgcg cccgcgacga tctcggaagc
     1801 cgaggccgag ccgccgttca ccagcaccac catcggcacc gtcttcgcct ccgccgggag
     1861 cttcctgagg tagtcttcct tcatgcggcc gcgcaggtaa tgctccgggt tcgcgttgag
     1921 cttcatcttc gagtcctcgg tgcggccgtc ggtgtacacc accagcgacc cggcgggcag
     1981 gaacgccgcc gacaccgcca ccgctccgtt gagcagaccc ccggggtcgt tgcgcaggtc
     2041 gagcacgatg cctttcatcg gcccctggtt ttccttgaag tgcctttcga tcgcgcgcgc
     2101 gagcgtctca ccggtgtgct cctggaactg gctcacccgg aaataggcat agcccggctc
     2161 gaggatcttg gatttcacgc tctggatctt gatgacggat cgcgtcagcg tcacgacgat
     2221 cggcttcggc tcgcccttgc gcacgatggt caacgtgatc tgggtgttgg gcttcccgcg
     2281 catgcgcttc accgcatcgt tcagcgtcat gcccttcacg gaagtttcat cgagcttgac
     2341 gatgagatcg ccgggcttga ttcccgcgcg cgaggcgggc gtatcgtcga tcggcgccac
     2401 caccttgacg aaaccgtctt ccatcccgac ctcgatgccg agcccgccga actcgccctg
     2461 cgtgccgacc tgcagctcct tgaaagcctc ctggtccaga taggcggaat gggggtcgag
     2521 cccggtcagc atgccgttga tcgcctcggt gatgagcttc ttgtcatcca ccggctcgac
     2581 gtaatcgctc ttgacgcggc cgaacacttc ggtgaacgcg cgcaactcct cgatcggcaa
     2641 cggcgctgtc gtcactgctt tctgcgccac cgccgagaaa ttgaggctca ccgccacgcc
     2701 gatcagcacg ccgatgacca acagcccgat ttgatgcaac ttgttacgca ttgccagctc
     2761 ctaagaaacc cgtgaagagt gaggtgtgag gggtgaggag tgaaaatccg ttctcaaatc
     2821 aaccctgcct cacgccaaac gcctcacacc tcacctgatg ttaacccagt ccagggggtc
     2881 cagcggcctg ccctggtgcc ggagttcgaa gtataaaccc gaatcgacgt tgccgccgct
     2941 cgcgcccacc gtggcgacgg gctcgccggc gctgatcggg tccccgaccc gcttgtagag
     3001 ggcctcgttg ttgccgtaga ggctcatgta ggcgtcgccg tggtcgacga tgagcaggtt
     3061 gccgaagccg cgcagccagt cggcgaacac cacgcgcccg tcggcgatcg cccgcacctc
     3121 ctcgcccgcc cgggcgacga tgaacagccc cttccagatc actccgccgt ctgcccgtgg
     3181 actgccgaag cggttggcaa gttccccgcg caccggcagg ctcagcctcc ccttcagctc
     3241 caggaacggg ctgtccccgc tggaggcctg cgggacgcgc tcgttcttca gccggggcgc
     3301 aggcttggcg cgcgccacca gtttcgacag gcgctcgacc agccgcgcga gccggctttc
     3361 gttgcgcttc aacgtgccga tctcgcgccg ctggcgctgg atgtcgcgcg agatccgggc
     3421 gagcacctgg ttgcgcgcgc gcttctcacg ctcgagacgg gcgcggaggc gctggctctc
     3481 ggcggtgacg gcggcgagtt cttccgcctt gcgccccgct tcctccgaga gctggcgcaa
     3541 ccgcacgagg ctctcgcgcg tctcggcgat cagccgggca cgcgcgcggg agatgtgacc
     3601 gaagtagtgg aggcggcgcg cgatctcgtt ggggtcctcg cggttgagca gaagcttcag
     3661 cggctcggac tgtccgccca ggtaatggtg gtagagcagg cgcgcgagca gcgtctgctg
     3721 cgccttcagc gcctgctcgc ggctccgcga ctcggcgtcg agctccgcga gccgctggtt
     3781 gatctcccgg gattgagcgc caagctcgcg cagcgcgcga ttcgcctccg agatcgcgcg
     3841 ctccgattcc ctcagggcgt cggctgcctc gctcttcgtt tcctcggcat ccgccaggct
     3901 cttctgcagc gcctcgatgc gtccgcgcaa ctcgcgcagc tccctctcct tcgcgctggc
     3961 gcccgccggc tccgcgcctg ggctcgccag cagcaggagg acaaggaccg cggccgagcg
     4021 cgctctcacg acgagaaggt gatgagggac ttcccggtca tctgcggcgg ctgcggcaga
     4081 cccattatgg cgagcagcgt cggggccacg tcctgcagcg cgccccggtc cgcgatgttt
     4141 gccttgcggc cgacgtaaag cagcggcacg agattgaggg tgtgcgccgt gtgcgcctgc
     4201 tttgtcacgg gatcgagcat ggtctccgca ttgccgtggt ccgccgtgat cagcacctcg
     4261 ccgccgattt cccgcatcgc cgcgaccgcg cgcccgatgc actcgtcgag cacctcgatg
     4321 gcgcgggtgg ccgccgcgag gttcccggta tgcccgacca tgtcgccgtt ggcgaaattg
     4381 cagacgatgg cgccgtattt ccggctcttg atcgcctcca ccagcctgtc cgtcacctcg
     4441 taggcgctca tctcgggctt gaggtcgtag gtggcgacct tgggggaggg gacgagcacg
     4501 cgctcttcgc cggggtacgg tttctccacg ccgccgttga agaaataggt cacgtgcgcg
     4561 tacttctcgg tttccgcgat gcgcagctgc ttcagccctt ttgcggaaat gtactcgccg
     4621 aaaccgcccg cgacgctctg gggctgaaac gccgccggga tgcgcgcaaa gtcctccccg
     4681 tagctggtga gcgtgcagta gtaacccagc ttcggaacgt gctttcgcgc aaattccttg
     4741 aagcccgggt cggtgagggc gcgcgtcagc tggcgcgcgc ggtccgcccg gaaattcatg
     4801 aagacgaccg cgtcgccgtc gttcatgcgc gcgcccgcgg acccgatgga ggtggccttg
     4861 acgaactcat cggtttcgcc gcgcgcgtag gccgcgtgca gaccctcgac cgcgctcccg
     4921 gcgcggtaat cggctcttcc ctgggtgatg agatcgtaag cgggttgcac gcgctcccag
     4981 cggccatcgc ggtccatcgc gtagtagcgg ccgcaaatcg aggcgatgcg gcccgctttg
     5041 agttcgccgc atttttcctg cagggcggca agcgaagctt ccgcgctcct gggcggggtg
     5101 tcgcggccgt cgaggaaggc gtgcacctgc acttccttca cgccggcgcg cgccgccagc
     5161 tcgagcatgg cgtggatttg caattcatgg ctgtgcacgc cgccgggcga aacgagtccc
     5221 aggatgtgca gcgcgcggcc ggcgccgcgt gcggtttcca ccgcttccag gagcgccgga
     5281 ttcctgaaga actctccggt ctcgatcgcg ttctcgatcc gggtgtattc ctggtacacg
     5341 atgcgccccg cgccgatatt catgtgcccg acttccgagt ttcccatctg gtccttcggc
     5401 agccccaccc atttctccga ggcgtcgatg atggtgtggg ggcaggtctg ccacaggaaa
     5461 ttccaatgcg gcttgcgcgc ctggcagatg gcgttgtgct cgcattcctc gcggtgcccg
     5521 aagccgtcga gtatgatcag gaggaccggt gtgactttca tgatttcacg ggttttcccg
     5581 cgtggcgtcg cctgctggca attctagact attttttcgc gccagcctgt tgctgatcgg
     5641 attggcgtgg acgctcccgt tcctgcagcc ctaccaccgc ttcccgctca cggcgttcta
     5701 cagcgaatgg ctggcattcg cgctcgggct ggcggcggcg ttggtattgc tttgcaagaa
     5761 accgtggcgg gaagcggcgc tgcccgccgt ggccatcgcg ccaattggat tgatcgtggt
     5821 gctcgggttg cacgtggcgc ttggacgcgt gccgtacccg gaacaggcgt tgaccgcggc
     5881 gctttacctg ttatgggcgg tcctgctggt cgtgctgggc catgcgctca ggcgcgtgtt
     5941 cgcgctgacg attatcgcaa caaccctcgc atggttcatg cttgctggcg gcgtgctcag
     6001 cgcgctgatc ggcctgttgc agcatcatca gctttcgacc ccgttcgatt tcctggtcgg
     6061 ccgcaaaatc gcgctgcagg tgtacggcaa tctggggcaa cccaatcatt acgccgccta
     6121 cctgttctgc gcgctggttt caacggttta tctgtacagc cgccgctgcc tgaatggcgc
     6181 catggctgcc ggtcccaccg cactcttcct gctggtgctg actcttgcgg cctcgcgcag
     6241 ttcctggctc tatcttggcg cgctcaccac gcttgcctgg ttgttgcacc gctacaggcg
     6301 cgacgccgag agccgcagac tcgctgtgat cgtgctgtgg cttctgcccg gtttcatcgt
     6361 cgcgcagtgg gtggcgacgc tgcccttcat gatgccgagc ggggcccgcc tggtgacatc
     6421 cgcggagcgc ctgtttcagg tggcgggcgg aatcgagcca cggctgcagc tctggcgcga
     6481 agcgtggggg ctgttcctcg gcgccccggt tctcggcgcg gggttcgggc agttcgcgtg
     6541 gcatcacttc ctgcaccagg cggcgaccgg cgcgacggcg gcgaccggcg tattcagcca
     6601 tgcccacaac atcgtgatgc agctcatggc tgaaacagga atagttggcg cgttgatcat
     6661 tgtcggcgca gttttactct ggctggcgga cctgaggcgt atccgtttcg gcctcgattg
     6721 gtggtggctg ctcgcgttgc tcgcaatcat cggcatccat agcctgctcg aataccccct
     6781 ctggtactcg tacttcctcg gcgtggcggc gctgctgctg gggtttggcg cagagcgcgt
     6841 gttcgccgtg cgatttgccg gagcggcccg cgttgtggcc ggactcggca tcatcgcggg
     6901 ttggttgaat cttgccctgg cgctggaacc ctatcgcgag ttcgaacgcc tggtatttac
     6961 tccggaaagc cgtgccgtgc gtcgaatgga tgaaacgacg ttcgcaaagg ctacagtcgc
     7021 cctgtaccgc gagccactgc tcgttccgta tgtcgatctc gcgattgcat acggtgttgc
     7081 ggtgaacgac gacaggctcg cggaaaaggt cgcgctcacc gcgcgtgccg cgcgcttcgc
     7141 gccggtgccc gtggtcgtct atcgccacgc attgctgctc gcgctcgcgg gcgagcgcaa
     7201 agcggcgctt gcccagctcg agcggtcgct acgcgtttac ccggaggaag ctggcgacat
     7261 catttcgcag ctagaggaac tcgcgcgccg ccgccctgac gaattcacac ccctgctgca
     7321 tttggcggtg gctaggggcg cagttcttcg cgcgcgtggc gcacgtgtat cacggtgaat
     7381 tgccgaccgt gatcaagatc atgggcaagc acgccgagtg gcatcccgca ggggcgacgc
     7441 ctctgataaa attggcgacc gccaagctcg cggaacggcg cacatcgctg ggcgggcgct
     7501 gacatgaccg gcaacgaagg gcgacagatt ggaacaattc ctggttttct tgcagaaaag
     7561 cccgtttaac atgctgctgt tcgggctggc gctggcatcg ggcggtatgc tcctctggcc
     7621 gctcgtctcg cggccgttcc ggcaggccaa tgaagtcggc gcgttcgagg cggtgcagct
     7681 catcaaccgg cgcgatgcgc tggtgctcga cattcgggac acgggagact atgcggccgg
     7741 gcacattacc aacgccaggc acattcccga ggcgcaactt cccgatcgca tgaaggagct
     7801 cgacaagcac aagtcacgcc cggtcatcgt cagttgccgc accggctcgc gggcgcccgc
     7861 ggtgagcggc atgctgcgca agcggggctt ctccgaggca ttcgcgctgc gcggcggcat
     7921 cgcggcgtgg cagcaggcga gcctgcctct ggaaaaaagc tgaatggcca aggtcacgat
     7981 gtacagcacg ggcatttgcc cgttctgcca gagggccgag cggctgctgc gggccaaggg
     8041 cgtttccgcg attgacaaga tccgcgtgga cctggaaccg gagcggcgcg tggaaatgat
     8101 ggaaaaaacc gggcgccgca ccgtccccca gatctacatc ggggagactc acgtcggcgg
     8161 ctatgatgag ctggccgcgc tggagcgcgc gggccagctg gacgggctgc tgcaagccgt
     8221 ctgaaacgtc cgcaaccttg acgagcgatc catgagcgag caacagccgg tctttaccat
     8281 cgagaaggtc tatctcaagg atttatcgct cgagatcccg aacgcgccgc aagtcttcac
     8341 cgagcgcgag gcgccgaagg tcgacatcaa tcttcacaat gaagcgcggt cgctcgataa
     8401 cggagtctac gaaatcgtgc tcactgcaac cgtgaccgca aagctgcagg acaagacggt
     8461 attcttggtc gaagtggcac aggcgggaat ttttcagatc cgcaatgtcc agcagcagga
     8521 gctggacttg ctgatcaacg tggcgtgccc cacgaccttg cttccctatg cgcgggagac
     8581 ggtcgcgacg atcctgaacc gcgccggttt ccctccggtg ctcctgcccc acgtgggctt
     8641 cgagaccctc tatcagcagc ggctgcagga gcagcaacaa cagcagcaac aagccggcgt
     8701 cccgcctgcg ccctgaaacc ggtgactggt gacccgtgat cagtgactgg acaaaaaacg
     8761 cctacctcag gggcacgcat cacctctcgg cgttaatcgt tgcctttttc tttgggaccg
     8821 gcgccggcgg cgccgctgct gctgctgatt tccgctcggt ggccgagaac gcggcggtgc
     8881 tttacgacgc gccctcggcg aaggcaaaaa agctctacgt ggtgagccac ggctacccgt
     8941 tcgaaatcgt ggtggtggtg gagggctgga gcaaagtgcg ggacgccaac ggggaactga
     9001 cgtggatcga gagcaaacat ctcgccgacc ggcgcacggt gctggtcaag gcaccgctcg
     9061 ctcaaatccg cgaggccgcc gacgacggcg ccgtggtggt attccaggcc cagcagaacg
     9121 tgttgctcga gctgctggaa gtggccggcg gctggctgcg ggtcaggcac cgcgacggcc
     9181 agagcggctt cgtccgcgcc gcgcaagtct ggggcgcctg aaccagtgtt gagtgatgag
     9241 tgtttagttt tgagttgtgc ctggctcgat tgactcaaca ctcaaaattc aacactcaac
     9301 actcaaaatt caacactccg catgaaaatc gcggttctcg gcgcgggcgc ctggggcacg
     9361 gcgatcagca tcaacctgtg cgcacgccat gaggtgcggc tgtgggcgcg cgatgccggg
     9421 ctgatcgagg cgctgcgtgt cgatcgcagc aaccggcgct atcttccggg tttcgcgctt
     9481 ccgtccgcgc tcgcgctcga caaggatctc ggggcgacgc tcaggggcgc tgaattgata
     9541 ctcatcgcag tgatcaccaa tggcctgcgc gacacgctgc aacgcatccg tgcggcgggg
     9601 ttcagcgccc ctctcgtctg gttgtgcaag gggttcgaaa gcgggagtgc ccggatgccg
     9661 caccaggtct gcgcggaaga actcgcgccc ggcatgccgc gcgccgttct gtccgggccg
     9721 agcttcgcgg aggaagtggc gcgggggctt cccgccgcgc tcacgctggc ttcgtccgat
     9781 ggcggttttg cgcgttcagc ggcgcgcgcc ctgcacggtg ccaggctgcg aatctacctg
     9841 agcgaggacg tggtcggggt cgaagccgcg ggcgcggtga agaacgtaat ggcgatcgcg
     9901 gccggggtga gcgacgggct cgaactaggc ctctcggcgc gtgcagcgct gatcacgcgc
     9961 gggcttgcgg agatgacgcg gctgggcgtc gcgctcggcg ggcgggcgga aacattcatg
    10021 gggctcgccg gcgccggcga tctgatactc acctgtaccg gggacctgtc gcgcaaccgc
    10081 aaggtgggtc tcgcgcttgc gcgcggcacg ccgctcgccg aggtgctcgc cggtctcggg
    10141 catacggcgg aaggcgtgct cacggcgcgt gcggtggccg gtctggcagg cgggcttggg
    10201 gtcgaaatgc cgatcacgcg cgcggtgtgc cgcgtgctcg acgacccccg gcaggcgcgc
    10261 gcggcggtgc aggagctgct cgcgcgcgaa caaaaaaccg aatactagcg gtcagcagtc
    10321 agctttcagc gttcggcaga atcgtcttgc cgcggcgaat cagggaaaac gttcttgctg
    10381 atagctgatc gctgaacgct gattgctaag gccggctgcc gcgaaaaccg agctggcgcc
    10441 aggcttcgta aaccacgatt gcgacggcat tggaaagatt gaggctgcgg ctggcctccc
    10501 gcatcggcac ccggatgcgg tgttcgggag gaaattccgc gagaatttcg ccaggcaggc
    10561 cgcgcgtctc cggaccgaac acgaacacgt cgtcttcacg atagcgcggc tcatcatgac
    10621 ggcgttcgcc gcgcgtcgtg acggcgaaca accgccggcc ggcaagcacg gaacggcacg
    10681 cgcgccaatc ctcgtggcgc acgatatccg ccaactcatg gtaatccaac cccgcgcgct
    10741 gcaattgctt gtcgcgcaac gagaatccga gcggatggac gaggtgcatg cgacagccag
    10801 tgttggcgca caggcgtatc acattgccgg cgttgggtgg aatttccggc tcgaatagca
    10861 cgatttcaaa catgcgcaag ttgccgcggg cgctaaaaca agatatcgtt acagatcaaa
    10921 ggcatgtaac gcgaaaatga tagagtactc ccgcccgggc aaaggcatga atgggacggc
    10981 ctgaaaaagt ccgcctcggc gagatattag tccagcaaag gctgctcacc gaagagcagc
    11041 tcaaaagcgc gctcgaggaa caaaagaaaa gcggccgccg gatcgggcgc gtattcatcg
    11101 agaagggctt cctcaccgaa gagcaaatct cggaggcgct ggcgcgccag ctcaacgtcc
    11161 cctacatcag cctcaagtcc tacaacaggc gcgaggacat caagcgcgag gtcgcgctca
    11221 agcttccgga aacgcaggcg cgccgctacc gcgcgctggt gctggaggac gccggcagga
    11281 cctacaaggt cggcatggtt gatcccaccg atctcatggc ctacgacgaa atcgcgagga
    11341 tcctcgagcg cgacatcgag atcgcggtgg tgaccgaaac cgaactgctg cgcgcgatag
    11401 accgcgtcta ccgccgcacc gagcagatca cggggctggc gcaggagctc ggggacgatc
    11461 tcggggagac caccgcagtg gattttggcg cggtggccct cacgccgggg ctcgaggaag
    11521 ccccggtggt gaggctgttg cagacggtgt tcgaggatgc ggtacaggcg cgcgcttccg
    11581 acatccacat cgagcctcag gaaaagaaac tgcagatccg cttccgcatc gacggcgtgc
    11641 tgcacctgca gacggaatcc gataccaaga tcgccacggc ggtggtgctg cggctgaagc
    11701 tcatgtcagg actcgacatt tccgagaagc gcctgccgca ggacggacgc ttcaacgtca
    11761 aggtgcgcaa cgcgcagatc gacgtgcggc tctcgaccat gccgacccag catggcgagt
    11821 cggtggtgat gcggctgctc aaccaggcga ccggcatgct ggggcttgat aacatcggca
    11881 tgccggccga catgctcgcc aagctgcgcg aggtcatcca tcgcccgagc ggcatggtgc
    11941 tggtgacggg gcccaccggc agcggcaaga ccaccacgct ctatgccgcg cttagcgaga
    12001 tcaataccac cgagcgcaag atcatcacgg tggaagatcc ggtggagtac cggctgcatg
    12061 gcatcaacca ggtgcaggtc aacgagaaga tcgagcttac cttcgaccgg gtgctgcgct
    12121 ccgcgttgcg ccaggacccg gacgtgatcc tggtgggcga gatgcgcgac aagccgacgg
    12181 tggagaccgg attgcgcgca gccatgaccg gccacatggt gttctccacc ctgcacacca
    12241 acgatgcggt gtccacgccg atccggctgc tcgacatggg cgcgccgcgc tacatggtag
    12301 cgctgtcgct gcagctggtg ctcgcgcagc ggctggtgcg cgtgatctgc gaaagctgcg
    12361 cccaggacta caccttgctg ccgaacgagc acgaatggct gcggcacgag ctcggcgacc
    12421 aggtggatca acatcgttat aagcgcggca aaggctgctc gcagtgcaac gccaccggct
    12481 acttggggcg cagcggcgtc tacgagatgc tggaaatgac tatccccgtg gttgaagcgg
    12541 cgaattccga cgatccggcg ctgttcgtgc gcgccgcgcg caagcagatg agcggcaata
    12601 cgctgcgccg ccacgcggcg cagctggtgg tggcgggcag aaccacggtt gaggaggcga
    12661 tacacgtcag cagtcagtct gacgattgaa ggcgtcgcgg atatcacgtc taaagtctcg
    12721 agatttttcg gaaggccgtt tttgatgcct cattttgcat acaagggccg tgacgaccgg
    12781 gggcaaccgg tgcagggcgt gctggaaggc gccgacagcg gcacggtggc ggggcaattg
    12841 ttcaacatcg gcatcacgcc gctcgagatc acgccttcca gcgcaccgcg gcagtccggt
    12901 cccggcctgt tccagcgcat gggtgagcag gcgatcaccc ataccgacgt tctgatgttt
    12961 agccgccaga tgtacacgct gctcaaggcc ggcgtcccga tcatgggagc gttgaagggg
    13021 ctgcaggaat cgaccgacaa caagaccttc gggcgcgtga tccaggaaat ccgcgaaagc
    13081 ctcgacagcg gacgggagct atccgcctcg ctcgcgcgcc atcccacggc gttcaattcg
    13141 ttttttgtca acatggtgcg ggtcggtgaa cacaccggca agctcgagga agtgttcctg
    13201 cgcctgtttc accacatgga gttcgagcag ttcatacgcg agcaggtgaa gtccgcaatc
    13261 cgctacccgt tatttgtcgt cgcgaccatg gcgatcgcga tagtgatcat caacctgttc
    13321 gtaatcccgg cctttgcaaa ggtttacgcg ggcttcaagg cggagctgcc gccgttgacc
    13381 aagatactca tcgccttctc cgacttcatg ctgactttct ggccggcgct gctggcggtg
    13441 ctgctggtgc tggtgttcgg cctccgcttc tatgtcaata cggtacaggg acagctggcg
    13501 tgggaccggt tcaagctcag gatcccgatc gctggcagga tcgtgctcaa ggcgacgctg
    13561 gcgcggtttg cgcgcagttt tgcgctggcg atcgagagcg gggtgccggt ggtgcaggcc
    13621 ctcacgatcg tcgccaacac cgtggaaaat gagtacgtgt cgctcaagat cgcggccatg
    13681 cgcgagagcg tggagcgcgg cgaatcggtg ctgcgcaccg ctgtggcaac gggcatcttc
    13741 acgcccatcg tgctgcagat ggtggcggtc ggcgaggaat cgggcgcgct ggatgacctg
    13801 atgaaggaaa tcgccgacat gtaccagcgc gaagtcgagt acgatctcaa gaaccttgct
    13861 gcgcagattg agccgatact gatcgtggcg ctcggcgtgc tggtgctgat cctggcgctc
    13921 ggggtgttcc tgccgatctg ggatctcggc acggcggcgt tcagcaaaaa gtgaccggcc
    13981 cggccagttc caggttcatg gttcgatgtt caaagttcaa ggttcagcgt tcgaggttca
    14041 cggacccggg acccggaact cgaaaccgcg agggggcggg ttcacgctgt tcgagctcat
    14101 ggtcgtaatc gtgattttca gcatgctggt ggcggtgctg ctcaaccggc tcggctacta
    14161 cctggaactc gcggagaagg cggccatgga gtcaacgctg cgggcgatca agaccgggct
    14221 gcagacccgg cttgcggaac tcatcatcac gcgccgccag gccgaaacgt caatgctcga
    14281 agtcgaagac ccgacgcgtt ggctggagca aaagccgccg aactatgcgg gggcgtatcg
    14341 cgagccggcg gaaccgggcc actggtactt cgacgagagg cagcggcagc tggtttatgt
    14401 cgtcaacagc gggcaacggc tcgagatcga gggcgctccg ggtgcaaagg aactgcggtt
    14461 tcgtgtgctg ttgctggtgg accgccatga ggccggaggc aagcgtgtcc aatcggtgag
    14521 cggagtgact ttggtcccgg ttcgcgccta tcgttggtca ggtgcggggc acgggcgtat
    14581 actcgcgcga gttttgcatc aatatcaaag gaatgcggat gttgattcgt gaacgatctc
    14641 acgtttcgcg ccgaaccgcg gggggcggct tcaccttgat cgagttggtc gtggtgatca
    14701 tcattttggg catactggcc gcggtggcgc tgccgcgctt cgccaatttg cagacgcagg
    14761 cgcgcatcgc gaagctgagc ggcgctctcg gcgcaatgca aggcgccgcg gctttggcgc
    14821 atgctgcgtg cctgacgtcc actccgcagt gcagcggcac tcagccgatg gaaggcgtga
    14881 acatcacgat gatcaatgcc tatccgacgg ccaacgctgc gggcattatc gcggccgcgg
    14941 gcttgaacgt cggcccgact tcacccgatg gctatgatgt cgcgggcggt ggacccgccg
    15001 cgggcgacgt gctgaccgtg atggcgctcg gaaacaatcc accgacctgc agcttcacgt
    15061 ataccgcccc ggcggcggga caggcgccgg tgttcagcac gctcactacg gcaggctgct
    15121 gaattgaaat gtttttaacc cgagtcgtac ttcatcttat agtataagga gcagggagaa
    15181 tgatgaattt tcatggtgac gcgaaagagg tgcgcattat gactgcatat caaaggggtt
    15241 tcaccctggt cgagttgatc gtggtgatcg tgattctcgg catactcgcc gcgaccgctt
    15301 tgccgcgatt catcaacgtt cagaccgacg cccgcatcgc cgcggtacag ggaatcgcgg
    15361 gcgggctgcg ctcggcggtg tcggtggttc aggcgcgctg gcaggtctcg ggcggcagcg
    15421 gcgtgacctc ggtgaacatg gccgatggga cagccgttgc ggttgccggc acgaccggta
    15481 taccgaccaa cgctgccggt ggaatcgggg ccgcaatgcg atgtgagtcg gcgacggcat
    15541 gtcagggcgt gaccatagac cttaccgtga gcccaagcac ctggcggccg tccggcggat
    15601 cggccacctg ccaggccagc tacgacccga ctgcgggcga cgtggtgaca accacgggtg
    15661 gatgttagat ctctgagatc gcagttcagg gcggtcgaga gcgcccgccc gtggtttgaa
    15721 cagggggtga aaggcggttc gcttcacccc cttgtttttt gggccgggca ggtcggttcg
    15781 cttcgaactc gcactatgcc gatatcctcc atgcgccaac cgcaagaagg cggcttcacc
    15841 ttggtggaac tggtggtcgt cattggtgtc gtggggctcc tggccgccat catcacgccg
    15901 cgcctgatcg gcacggacgc gttcgcctcg cgcggcttct acgataaggc gcaggcggtg
    15961 gttcgctacg cgaacaaaac ggcgatcgcg tggcgacggc cgatcttcgt gtgcgtcacg
    16021 gcgaccagta tcagtgccgg cggagccgcc ggctgcggct cgcccctcgt caatcccgcc
    16081 accggcggcg cgctcacggc gacggcaccg agcggagtga cgctgtcacc ggccagcttc
    16141 agcttcgaca gcgcgggccg ccccaatccg aacagcacga tcacgatcgt ggtgacgagc
    16201 gcgatagccg gagatcccgc gcggcagatc gtggtcgagg ccgaaacggg ttatgtccat
    16261 ccctgaatcc agttccgagt tccgagttcc gagttccgag tttaaggaac aggcaaaacc
    16321 gcgctcgccg attaactgga aactgaaaac tggtaactcg agacgacgaa agtccctcgg
    16381 attttcgctc gtcgagcaaa tcgtattcat cgtcgtggtg ggcgtcgcgg tggcgggcgt
    16441 gctgctcgcg ctgaacgtgg tcacgcgcgg cagcgtggat ccggtggtcc agaaacaggc
    16501 gctggccatt gccgaggccg tgctggaaga ggttcagcta cagcctttca cctactgcga
    16561 tccggacgac gccaatgcgg cgaccgcgct caatgccgcc ggatgcgcga caacggccga
    16621 ggccctcggt cccgagccgg gcgagtcgcg tctcagcgcc accgcgccgt tcgacaacgt
    16681 caatgactac cacggcttca gcatgagcgg cggcattacc gacattgcgg gcgattccat
    16741 ctccgggctt ggcagctact cggtgtcggt aagcgtggcg gggcaagcgc ttggcggcat
    16801 cgcttcgagc gatgcgctcc tgatcacggt cacggtcacg catcctgcca tcactccagg
    16861 tctcgtgctc cacggctacc gcgtgcgcta tgcacctaac gcattgccgt gaccgagcgt
    16921 tgatgatgca aacggttccg aatcacggat cacgaatcgc gaacctcgga attgggtgcg
    16981 cacgcggcgt gacattagtc gagatgatcg tggtgatcgc gatcaccggt attatcgcgg
    17041 caggggtggc actgttcata cgccgcccgg tggaaggcta catcgatgcc gcgcggcgcg
    17101 ccgagctctc cgacattgcc gataccgcat tgcggcgcat cacgcgcgac ctgcgcaccg
    17161 cgctccccaa cagcatccgt atcaccgaat ctcctcctgc gagcggtgtc ttctacctgg
    17221 aatacctgca gaccagcggc ggcggacgct accgggcgga agtggacagc ggcgggatgg
    17281 gagacatact cgcttttact gcggccgact cgacgttcga cgtgatcggc acgatgccga
    17341 cactggccgc gggcgatttg atcgtgatct acaaccttaa tgccgatccc gcgatcccca
    17401 ccgcgaacgc ttactccggg gacaaccgcg ccgcttattc gagcaacacc gccaccacca
    17461 tcacgcttgc cgcggcacat cagttcccat ttgcctcccc gggcaagcgg tttcacgctg
    17521 tgcaataccc ggtgacctac gaatgcaacc ccggaaccgg ggcgctgcgg cgctactgga
    17581 actatgcgat cgccgcggcg cagtcgacgc cgcccgtcac gccaaacaac gcactactcg
    17641 cgaccagc
//
LOCUS       DBNGWZ010000204        14296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2918686, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000204 DBNGWZ010000000
VERSION     DBNGWZ010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2918686"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="AC16CX_06030"
     CDS             complement(<1..1610)
                     /locus_tag="AC16CX_06030"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3012107.1"
                     /translation="MADYKKTLNLPDTRFPMRGDLAKREPLMLKSWQERRFYQRIRAA
                     RRGAPRFILHDGPPYANGDIHIGHAVNKILKDIIVKVKTLSGFDAPYVPGWDCHGMPI
                     EVQIEKEHGKNLPTRETQRLCRAYAAEQIERQKKDFQRLGVLGDWERPYLTMAYQNEA
                     DEIRALGILLRKGYVYRGLKPVNWCFDCGSALAEAEVEYEDRTDIAVDVGFPFAEPEK
                     VAKAFGLARLPDQPGYAVIWTTTPWTLPGNQALNVHPEFTYNLVRTERGLLILAADLR
                     EACLARFGLAELEVMGECKGRALEGIRFRHPFYDRLAPVYLGDYVTLDTGTGIVHSSP
                     AYGVEDFESCRRYGMKDEEILTPVMGDGRFVANLPLFGGMMIWDANPKIVKTLEERGV
                     LFDKRSWDHSYMHCWRHKTPVILRATVQWFAGMDEVPGHNGVKPAEPLRATALRGVEA
                     TQFYPAWGQARLHGMIANRPDWTLSRQRQWGVPMPFFIHKETGMLHPRALELLEQVAK
                     RVEHGGIESWQSLEAEELLGDDAARYEKV"
     gene            complement(1653..2609)
                     /locus_tag="AC16CX_06035"
     CDS             complement(1653..2609)
                     /locus_tag="AC16CX_06035"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR3012108.1"
                     /translation="MRVTRGIPAAAAVPAALTIGNFDGVHLGHQAMLAELKRAAGRLG
                     VPACVLTFEPHPREFFAPDQAPTRLTSLREKLELLAGCGMDRVHICRFNYGFAQTAAE
                     EFIECIIVRGLGARWLLAGDDFRFGARRAGDLVMLKQAAPRFGFEIAALASVTLDGER
                     VSSTTVRDALAAGELGRAGRLLGRAYSISGRVVAGDGLGRKLGFPTANVQMKHNRPPL
                     TGIFAVRLHGVARGPLRGAASLGVRPTVKQQGTPVLEVHVLDFDGELYRRHVRVEFLH
                     KLRDEEKYADLATLTRQIARDVENARQYFERESAMARRKAGS"
     gene            complement(2692..3456)
                     /gene="cobA"
                     /locus_tag="AC16CX_06040"
     CDS             complement(2692..3456)
                     /gene="cobA"
                     /locus_tag="AC16CX_06040"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3012109.1"
                     /translation="MKQTVGKVYLVGAGPGAPDLITLRGAEVLKRADIVFHDALVHAE
                     VLALATRAEKLAVGKRCGKFTTAQQFINKRLVDAARKHEVVVRLKGGDPMLFGRAQEE
                     IAALEAAGVPYEVVPGVTSALAAAAEAGVSLTQRGIARSVAFVTPRVGEGEAPNNWVS
                     SAASADTAVIYMGAGQAETIASALIAAGAPRDLPILVAENATLPHARRIALTLEELPE
                     IARYGITGPTLILAGRAFAAALAASGQETLRKHAQA"
     gene            complement(3461..3994)
                     /locus_tag="AC16CX_06045"
     CDS             complement(3461..3994)
                     /locus_tag="AC16CX_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HIR3012110.1"
                     /translation="MAIIIKDREVKTDLWLLLKPADDGSVPAVPPAGDVIVPLVLWQT
                     QRAELLAHPGRIGVWLNSHEEPAAIAEDLRIFGVVAVNFPKFGDGRGYSIARLLRERY
                     GYRGELRAIGDVFRDQLFFLSSCGFNAFVLRAGEDPQEALAAFSDFSEAYQSSVERPL
                     PLFRRRYLASPDGMEWS"
     gene            complement(3939..5654)
                     /locus_tag="AC16CX_06050"
     CDS             complement(3939..5654)
                     /locus_tag="AC16CX_06050"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HIR3012111.1"
                     /translation="MYRYDEIDQRLVEERVVQFRDQTRRFLAGKLSEDDFRPLRLRNG
                     LYIQRYAPMLRVAIPYGLLSSRQLRTLAYIARKWDRGYGHFSTRQNIQFNWPKLEDVP
                     DILAELAKVQMHAIQTSGNSVHNITTDHFAGVARDEIVDSFAWCELLRQWSTFHPEFS
                     YLPRKFKIAVNGAKADRAATYLHDIGLHAVRNAAGEVGFRVIVGGGMGRTPIIGHVIS
                     EFLPWPHLLTYIEAILRVYNRYGRRDNLNKARIKILVKERTLERFREEVEAEWAHLKD
                     GPSTLTEEEVRRVEGRFTRPAYERLPDDDAGYGARLAAEPAFAAWVKRNVHPHKALGY
                     AAVTLSLKKTGVPPGDVTSDQMDAIADLADRYSSGELRMSHEQNLILADVRQSELHAL
                     WREAKAQGLATPNIGLLTNIICCPGGDFCSLANAKSIPVAQAIQERFDRLDYLYDIGE
                     LDLNISGCMNSCGHHHVGHIGILGVDKNGEEWYQISIGGNQGLVRPGAPAALGKVIGP
                     SFARVDIPDVIEKLIETYLERRESDEERFIDVVWRVGIEPFKERVYGNHHQRQGSQDR
                     SLVAA"
     gene            5765..6706
                     /gene="cysB"
                     /locus_tag="AC16CX_06055"
     CDS             5765..6706
                     /gene="cysB"
                     /locus_tag="AC16CX_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HIR3012112.1"
                     /translation="MKLQQLRYLREVAKRGLNLSEAAEALHTSQPGISKQIRQLEDEL
                     GVDILVRHGKRVVDMTEPGRVILGITERILQDAENLKQVGREFGTEGAGSLTIATTHT
                     QARYALPPVIARFSQRYPKVRLSLRQGSPPYIAELVESGEADIAIATEAAEFYHELVL
                     LPCYRWNRCVITPLKHPLLKEKQLTLEAIARYPIITYDFAFTGDSPIKRAFDNKGIAP
                     NVVLTAIDADVIKAYVEMGLGIGILAKMAFDPARDMGLRLIDASHLFEPSTTRIVVRP
                     NAYLRGYVYDFIELFAPHLKRAVVDATLKGEGSSYEL"
     gene            complement(6812..7330)
                     /locus_tag="AC16CX_06060"
     CDS             complement(6812..7330)
                     /locus_tag="AC16CX_06060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR3012113.1"
                     /translation="MIPALRFAARCLLGLAIASVAVEAADLKLWAGGPTPLLDLRDLE
                     GRRHRLADYRNQVVLVNFWATWCGPCRDEMPSIQRLKEKLAGRPFTVLAVNLDEPEAR
                     IRKFLSEVKVDFTVLLDPEMKMAKAWEARILPASYIIGPDGRIRYNLVGELNWDHDLV
                     VSRLSELLPAGK"
     gene            complement(7380..8468)
                     /locus_tag="AC16CX_06065"
     CDS             complement(7380..8468)
                     /locus_tag="AC16CX_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR3012114.1"
                     /translation="MDRRTFIKTTAAVPAAVAVSGLPRIATASGPWRVFEVTTRAEVF
                     KPAGAARIWLPVPLTNDTDYQKNLGSTWTAEGAKTVLTVDNKYQAGIVQAEWPESAGN
                     QALTLVSRFATRDRAVDLSVRGSPVTEDPAVLKLNLEPTELIPTDGIVRDSAREITKG
                     ARTDIEKARVIYEWMVDNTFRDPKVRGCGWGDIKAMLETRNFGGKCGDLNTLFVGLAR
                     SVGIPARDVYGVRVAPSNHGYRSLGANSEVISKAQHCRAEFYAQSHGWVPVDPADVRK
                     VVLEEPPGNLALNDDKVTRVRRMLFGAWEMNWLAFNYAHDVRLPGSKGPKIPYFMYPN
                     GETAEGRLDQLEPDTFKYTMTARELKGA"
     gene            complement(8818..10356)
                     /gene="murJ"
                     /locus_tag="AC16CX_06070"
     CDS             complement(8818..10356)
                     /gene="murJ"
                     /locus_tag="AC16CX_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3012115.1"
                     /translation="MNLLKTLATVSSMTLISRILGFVRDLVIARVFGAGLATDAFFVA
                     FKLPNLLRRLFAEGAFSQAFVPILSEYKSRRGGQETRLLVDHVAASLAAALFVVTLIG
                     VLAAPLIIYVTAPGFTATPGKFDLTVGLLRVTFPYIFFISLTALAGGILNTYSRFSVP
                     AVTPTLLNLSFIGFALWGAPYFDPPVKALAWAVFAGGIAQLALQIPFLARLGLLPRFR
                     LDLKDEGVWRVLRQMGPAVFGVSISQVSLVINVIFASFLVTGSVSWLYYADRLMEFPI
                     GLLGVALGTILLPSLSRYHAERSPDQYSELLDWGLRLTLLLAAPAAVALALLAVPLVA
                     TLFHYGAFSAEDVFATRRAVIAYSVGLIGLVMVKVLAPGFYARQDIRTPVRMAIVTLA
                     LTQLMNFAFIWPFKHAGLALAIGLGACVNAALLLRGLLRRNIYAPRPGWPVFLMKLVI
                     AVYAMASVLWLVSGTDASWLAVGAGTRAGNLVGVIILGAATYFAALWLLGFRIADFSK
                     RGVE"
     gene            10446..10709
                     /gene="rpsT"
                     /locus_tag="AC16CX_06075"
     CDS             10446..10709
                     /gene="rpsT"
                     /locus_tag="AC16CX_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3012116.1"
                     /translation="MANIASARKRARQAEKRRLHNAALRSELRSAIRNVRKAIAAGDK
                     KAARTVFERAAGVIDSIADKRIIHKNAAARHKSRLAAGIKAMT"
     gene            complement(10866..11186)
                     /locus_tag="AC16CX_06080"
     CDS             complement(10866..11186)
                     /locus_tag="AC16CX_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HIR3012117.1"
                     /translation="MFPGSPEIVIQGITESGHSFRPSDWAERLCGMMSVFGQDRHLSY
                     SPYLKPIMAAGVRCVVVDLRLEELDPDAFRFLLGFARDNELKMRPGRKEKRPEEVASG
                     PATS"
     gene            11379..12584
                     /locus_tag="AC16CX_06085"
     CDS             11379..12584
                     /locus_tag="AC16CX_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HIR3012118.1"
                     /translation="MTHVMDTYARLPVAFERGEGSWLWDASGKRYLDAVAGVAVCGLG
                     YGHPKYVAALREQVGKLVHTSNLYRITLQEKLADRLAAISGMDNAFFCNSGCEANEAA
                     IKLARLYGHKKNIEAPAIVVMEKAFHGRTIATLSATGSRKVQAGFEPLVAGFVRVPFD
                     DLEAVKRVAENNRSVVAVLVELLQGEGGVNVCHDDYLNGLREICDANGWLLMLDEVQS
                     GMGRTGKWFAFQHSGVAPDVMPLAKGLGNGMPIGACLAAGAAAQLFKPGSHGSTFGGN
                     PLACAAALATLDIIEQERLMENAVRIGDFIRGELARRLGGLKGVKEIRGKGLMIGVEL
                     EFPCGELVQQALERGALINVTVDNVVRLLPPLVFKREEAELLLATLVPLVTEFLDRQS
                     APRAAAQPA"
     gene            12613..13554
                     /gene="argF"
                     /locus_tag="AC16CX_06090"
     CDS             12613..13554
                     /gene="argF"
                     /locus_tag="AC16CX_06090"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3012119.1"
                     /translation="MAVRHYLQFRDFSREEYEYLFERTRWIKDMFKRYVPYQPLRDRT
                     LAMVFEKHSTRTRVSFEAGMHQLGGTVITLMTRDTQMGRGEPMEDVARVLTRMVDIVM
                     IRTFEQAIIERFAEHSRVPVVNGLTNEYHPCQILGDLYTYLEHRGSIRGRTVAWIGDS
                     NNVCNTWLQAAEVFDFNVHVSTPPGYEVEPERAGLYGTDHYEVFEDPMKAAKGADLIT
                     TDVWTSMGDESEDAERKRDFEAWRVDKEMMRAAKKDALFMHCLPAHRGEEVAAEVIDG
                     PQSVVWDEAENRLHTQKALMEYLLLGRIKTQGDLWST"
     gene            13653..>14296
                     /locus_tag="AC16CX_06095"
     CDS             13653..>14296
                     /locus_tag="AC16CX_06095"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3012120.1"
                     /translation="MDKVKKVVLAYSGGLDTSVILKWLQDTYDCEVVTFTADIGQGGE
                     LEPARRKARKLGVKRIFIEDLREEFARDFVFPMFRANAVYEGEYLLGTSIARPLIAKH
                     LVEIAKKTGADAIAHGATGKGNDQVRFELGAYALMPDVRIIAPWREWDLTSREKLLEY
                     AEAHGIPVEGKKKQGGAPYSMDANLLHISYEGGILEDPDYEPEESMWRITVPPE"
BASE COUNT         2651 a         4610 c         4619 g         2416 t
ORIGIN      
        1 accttctcgt agcgcgccgc gtcgtcgccg agcagctcct ccgcctcgag cgattgccag
       61 ctttcgatgc cgccatgctc gacgcgcttg gcgacctgct cgagcagctc gagcgcgcgc
      121 gggtgcagca tcccggtctc cttgtggatg aagaacggca ttggcacgcc ccactggcgc
      181 tggcgcgaca gcgtccagtc ggggcggttc gcgatcatgc cgtgcaaccg cgcctggccc
      241 catgccgggt agaactgcgt cgcctccacc ccgcgcagcg cggtcgcccg cagcggctcc
      301 gccggcttca cgccgttatg gccgggcacc tcgtccatgc cggcgaacca ctgcaccgtg
      361 gcgcgcagga tgaccggcgt cttgtggcgc cagcagtgca tgtagctgtg atcccagctt
      421 cgcttgtcga acagcacgcc gcgctcttcc agcgtcttca cgatcttcgg attggcatcc
      481 cagatcatca tgccgccgaa cagcggcaga ttggcgacaa acctgccatc gcccatcacc
      541 ggcgtgagga tctcttcgtc cttcatcccg tagcggcggc aggattcaaa gtcctccacc
      601 ccgtacgccg gcgacgagtg cacgatgccg gtgccggtat cgagcgtgac gtagtcgccg
      661 agatagaccg gcgcaaggcg gtcgtagaac gggtgcctga atcggatccc ctcaagcgcc
      721 ctgcctttgc actcgcccat tacctccagc tcggcaagcc cgaatcgcgc caggcatgcc
      781 tcgcgcagat cggcggccag gatcagcaat ccgcgttcgg tgcgcacgag gttgtaggtg
      841 aattccggat gaacgttgag tgcctggttg ccgggcagcg tccacggcgt cgtggtccag
      901 atgaccgcgt agcccggctg gtccggcagc ctggccagcc cgaaggcctt tgcgaccttc
      961 tccggttcgg cgaacgggaa accgacgtcg acggcgatgt cggtacggtc ctcgtactcg
     1021 acctcggcct cggcgagcgc ggagccgcag tcgaagcacc agttgacggg cttgagcccg
     1081 cggtacacgt accctttcct gagcaggatg ccgagcgcgc ggatctcgtc ggcctcgttc
     1141 tggtaggcca tggtgagata ggggcgctcc caatcgccca gcacgccgag gcgctggaaa
     1201 tccttcttct ggcgctcgat ctgctcggcg gcataggcgc ggcacaggcg ctgggtctcc
     1261 cgcgtcggca ggttcttgcc gtgctccttc tcgatctgca cctcgatcgg catgccgtgg
     1321 cagtcccacc ccgggacgta cggcgcgtcg aatccggaca gcgtcttcac cttgacgatg
     1381 atgtccttga ggatcttgtt gaccgcatgg ccgatgtgga tgtcgccgtt ggcgtacggc
     1441 ggcccgtcgt gcaggatgaa acgcggcgcg ccgcggcgcg cggcgcggat gcgctggtag
     1501 aacctgcgct cctgccagct cttcagcatc agcggctcgc gcttcgcgag atcgccgcgc
     1561 atcgggaacc gggtgtcggg gaggttcagc gttttcttgt aatcagccat cgagaatcag
     1621 cggtcagctg tcagctacca gaaaacgggc agctacgatc cggcctttcg tcgcgccatt
     1681 gcgctttcgc gttcgaagta ttggcgggcg ttctcaacgt cgcgggcgat ctggcgcgtc
     1741 agggtcgcga gatcggcgta cttctcctcg tcgcgcaact tgtgcagaaa ctccacccgc
     1801 acgtggcgcc ggtaaagctc gccgtcgaaa tcgagcacgt gaacttccag caccggcgtc
     1861 ccctgctgct tcaccgtcgg gcgcacgccg aggcttgccg ccccgcgcag cggcccgcgc
     1921 gcaacgccat gcagccgcac cgcgaagatg ccggtgagcg gcggccggtt gtgcttcatc
     1981 tgcacgttgg cggtcgggaa gccgagcttg cgccccagcc cgtcgccggc gaccacgcgc
     2041 ccgctgatgg agtaggcgcg gccgaggagc cggccggcgc gtccgagctc gcctgcggcg
     2101 agcgcatcgc gcaccgtcgt gctcgacacc cgctcgccgt cgagcgtcac gctcgccagc
     2161 gccgcgatct caaagccgaa gcgcggcgcg gcctgcttca gcatcacgag gtcgccggcg
     2221 cgccgcgcgc cgaagcggaa atcgtccccc gccagcaacc agcgcgcgcc gagcccgcga
     2281 acgatgatgc actcgatgaa ttcctccgct gcggtctgcg cgaaaccata gttgaagcgg
     2341 cagatgtgca cccggtccat gccgcaaccc gcgagcaact ccagcttttc gcgcagcgag
     2401 gtgagccggg tcggcgcctg gtcgggggcg aagaactcgc gcgggtgcgg ctcgaaggtg
     2461 agcacgcagg cgggcacgcc gaggcggccg gccgcgcgct tcaactcggc cagcatcgcc
     2521 tgatggccga gatgcacgcc atcgaaattg cctatggtga gcgcagccgg gacggctgcg
     2581 gcggcaggaa tgccgcgggt aacgcgcata caaccaagcc ggaaatgtta ctaaaagctt
     2641 gaattgtagc gtgttttagg agaacgggtc cagcggatcg agcgtgcggg ctcacgcttg
     2701 cgcgtgctta cgcagggtct cctgcccgga cgcagcgagc gcagcggcaa atgcacgtcc
     2761 cgccaggatg agcgttggcc cggtgatgcc ataccgagcg atttccggca attcctccag
     2821 cgtcagtgcg atgcgccggg catgcggcag cgtcgcgttc tcggcgacca ggatcgggag
     2881 gtcgcgtggc gcgccggcgg cgatcagcgc cgaggcgatg gtttcggcct gtcccgctcc
     2941 catgtaaatg acggcggtgt cggcggacgc agcgctgctc acccagttat tcggagcttc
     3001 tccctctccg acgcgtggcg tgacaaatgc gacactgcgt gcgatgccgc gctgcgtcag
     3061 tgaaacgccc gcttccgccg ccgcggcaag cgcggaggtg acgccaggca cgacctcgta
     3121 gggcacgccc gcggcttcca gtgccgcgat ttcttcctgc gcgcgcccga acagcatcgg
     3181 atcgccgccc ttgagacgta ctaccacctc gtgcttgcgc gcggcgtcca ccaggcgctt
     3241 gttgatgaat tgctgggccg tggtgaactt gccgcagcgc tttccgacgg cgagcttctc
     3301 ggcgcgggtg gcaagggcca gcacttccgc gtgcacgagc gcatcgtgga acacgatatc
     3361 ggcgcgcttc aggacctccg cgccgcgcag ggtgatgagg tccggcgcgc cgggcccggc
     3421 gcccacgaga tagaccttgc cgaccgtctg tttcatggtg tcacgaccat tccatgccgt
     3481 caggggaagc gagatagcgc cggcgaaaaa gaggcagcgg gcgctcgacc gagctttggt
     3541 aggcctcgct gaaatcgctg aacgcggcga gcgcttcctg cgggtcctcg cccgcgcgca
     3601 ggacgaaggc attgaagccg caactggaga ggaaaaacaa ctggtcgcgg aatacgtcgc
     3661 cgatcgcccg cagctctcct ctgtagccgt agcgctcgcg cagcagccgc gcgatggaat
     3721 aaccgcggcc gtcgccgaat ttcgggaaat tcaccgccac gacgccgaag atccgcagat
     3781 cctcggcgat ggccgccggc tcttcgtggc tgttcagcca caccccgatc cgcccgggat
     3841 gggcgagcaa ctccgcgcgc tgggtctgcc acaggacgag tggcacgatg acatcgcccg
     3901 ccggcggcac cgccggcacg ctgccatcgt ccgcgggctt aagcagcaac cagagatcgg
     3961 tcttgacttc cctgtctttg atgatgattg ccatagacac gctccttgaa gggttcgatg
     4021 ccgacgcgcc acacgacgtc gatgaagcgt tcctcgtcgc tctcgcgccg ctcgagataa
     4081 gtctcgatca acttctcgat cacgtcgggg atgtcgacac gggcgaaaga gggcccgatc
     4141 acttttccga gcgcggccgg cgcgccgggg cggacaagcc cctggttgcc gccgatggaa
     4201 atctgatacc actcctcgcc gttcttgtcc acgccgagga tgccgatgtg gccgacatgg
     4261 tggtggccgc acgaattcat gcagccggag atgttgagat ccagctcgcc gatgtcgtag
     4321 aggtagtcga ggcggtcgaa gcgctcctgg attgcctgcg ccaccgggat ggacttggca
     4381 ttggcgagcg agcagaaatc gccaccgggg cagcagatga tgttggtgag gaggccgatg
     4441 ttcggcgttg cgaggccctg cgctttcgct tcccgccaca acgcgtgcag ttccgactgg
     4501 cgcacgtcgg caaggatcag gttctgctcg tgggacatcc gcagctcgcc gctactgtag
     4561 cggtcggcaa ggtcggcgat tgcgtccatc tgatcggagg tgacgtctcc cggcggcact
     4621 ccggttttct tcagtgacaa cgtcaccgcc gcgtatccca gcgccttgtg cggatggacg
     4681 ttgcgcttca cccacgcagc aaaggccggt tcggcggcaa gccgtgcccc gtacccggca
     4741 tcgtcatcgg gaagccgttc atatgccggc cgggtgaaac gcccttccac gcgccgcact
     4801 tcctcctcgg tgagcgtgga aggcccgtcc ttcagatgcg cccattcggc ctcgacctcc
     4861 tcgcggaacc gttccagtgt gcgctccttc acgagaatct tgatgcgcgc tttgttgagg
     4921 ttgtcgcgcc ggccatagcg gttatagacg cgcaggatcg cctcgatgta ggtaaggagg
     4981 tgcggccacg gcaggaactc gctgatcacg tggccgatga tgggggtgcg ccccatgccg
     5041 cccccgacga tcacccggaa gccgacctcg cccgcggcgt tgcgcaccgc gtgcaggccg
     5101 atgtcgtgca ggtaagtggc cgcgcgatcg gccttcgcgc cgttaaccgc gatcttgaac
     5161 ttgcgcggca ggtaggagaa ctcggggtgg aacgtggacc actggcgcaa cagctcgcac
     5221 caggcgaagg aatccacgat ctcatcgcgt gcgacgccgg cgaagtggtc ggtggtgata
     5281 ttgtgcacgg aattgccgct ggtctggatt gcgtgcatct gcaccttcgc gagctcggcg
     5341 aggatgtcgg gcacatcctc gagcttcggc cagttgaact ggatgttctg acgcgtgctg
     5401 aagtgcccgt agccgcggtc ccatttgcgg gcgatgtacg ccagggtgcg cagctgtcgc
     5461 gatgagagca gcccgtacgg gatcgccacg cgcagcatgg gcgcgtagcg ctggatgtag
     5521 aggccgttgc gcagcctgag cggacggaaa tcgtcctccg acagcttgcc ggcgaggaaa
     5581 cggcgcgtct ggtcgcgaaa ttggacgacc cgttcttcca ccaggcgctg gtcgatctcg
     5641 tcgtaccggt acaccgcgtt tttcccgtca aatctttcga attggcatgg tagcaaccgc
     5701 ctgttatact tcaaaagaat attgtattag actgtaataa ctatttagta tttacttcaa
     5761 ctctatgaaa ttacagcaat tacgctatct gcgggaggtg gccaagcgcg gacttaacct
     5821 ctccgaggct gccgaagcac tccacacctc gcaaccgggc atcagcaagc agatccggca
     5881 actggaggac gagctggggg tggacatcct ggtacgccac ggcaagcggg tcgtggatat
     5941 gaccgagccg ggacgcgtga tcctcgggat taccgagcgc atcctgcagg atgccgagaa
     6001 cctgaagcag gtcgggcggg agttcggcac cgagggcgcc ggctcattga cgatagcgac
     6061 cactcacacc caggcgcgct atgcgctgcc gccggtcatc gcccgcttta gccagcgcta
     6121 ccccaaggtg cgcctctcgc tgcggcaggg cagcccgccg tatatcgcgg agctggtcga
     6181 gtcgggcgag gccgatattg cgatcgccac cgaggccgcc gagttctacc acgaactggt
     6241 gctgctgccc tgctatcggt ggaaccgctg cgtgatcacg ccgctcaagc atcctctgct
     6301 caaggagaag cagctcacgc tcgaggcgat cgcgcgctat cccatcatca cttacgactt
     6361 cgcgttcacc ggcgactccc ccatcaagcg cgcgttcgac aacaagggaa tcgcgccgaa
     6421 cgtggtgctg acggcgatcg acgccgacgt gatcaaggcc tacgtcgaga tggggctggg
     6481 catcggcatc ctcgccaaga tggcgttcga tcccgcgcgc gacatgggct tgaggctgat
     6541 cgacgcgagc cacctgttcg agccgagcac gacgcgcatc gtcgtgcgtc ccaacgccta
     6601 cctgcgcggt tacgtctacg acttcatcga actatttgcc cctcacctca agcgcgcggt
     6661 ggtggacgcg accctcaagg gcgagggctc aagctacgaa ctctgatcaa gcgtgattgg
     6721 tgattcgtga ttggtgagtg gattgcttcc cgagcgcagg agataaacgg cgctgtccaa
     6781 tcactattca ccgttcacta ttcacgcctt tctacttgcc agcgggcaag agctcggaaa
     6841 gacggctgac gaccaggtcg tggtcccagt taagctcgcc gaccaggtta tagcggatcc
     6901 tgccgtcggg cccgatgatg tagctcgccg gcagtatgcg ggcttcccac gccttggcca
     6961 ttttcatttc agggtcgagc agtacggtga aatccacttt gacctcggac aggaatttcc
     7021 ggattcgggc ctcgggctcg tccagattga ctgcgagcac ggtgaatggc ctgcccgcca
     7081 gtttttcctt cagccgctgg atcgagggca tctcatcccg gcatggcccg caccaggtcg
     7141 cccagaaatt caccagcacc acttggttgc gatagtcggc gagccggtgc cgtcggccct
     7201 cgagatccct gaggtcgagc aggggtgtgg gaccgccggc ccacaacttg agatcggccg
     7261 cctcgaccgc gactgatgct atggcaagcc cgagcaggca gcgggccgca aacctaaggg
     7321 ccggaatcat gggattggaa aagcggcgat gcgcttccaa agcgcaccgc cgcttcgggt
     7381 tacgcgccct tcagctcgcg cgcggtcatg gtgtatttga aggtgtcggg ctccagctgg
     7441 tccagccgtc cttccgcggt ctcgccgttc ggatacatga agtacgggat tttcggcccc
     7501 ttcgatcccg gcagccgcac gtcatgcgcg tagttgaacg cgagccagtt catttcccat
     7561 gcgccaaaca gcattctgcg cacgcgggtc accttatcgt cgttcaacgc gagattgccc
     7621 ggcggctcct ccagtaccac cttgcgcacg tcggccggat cgaccgggac ccaaccatgg
     7681 ctctgggcat agaactcggc gcggcagtgc tgtgccttgc tgatcacctc gctgttcgcg
     7741 ccgagactgc ggtagccgtg attggacggt gcgacgcgca cgccgtagac gtcgcgtgcg
     7801 ggaatgccaa ctgaacgggc caatcccacg aacagcgtgt tcaggtcgcc gcacttgccg
     7861 ccgaagttgc gcgtctcgag catggccttg atgtcgcccc agccgcagcc gcgcaccttc
     7921 gggtcgcgga aggtattgtc caccatccat tcgtagatca cgcgtgcctt ctcgatatcg
     7981 gtcctcgcgc ccttggtgat ctcgcgcgcg gaatcgcgca cgatgccgtc ggtcggaatg
     8041 agctcggtcg gctcgaggtt gagcttgagc accgcgggat cttccgtgac cgggctaccg
     8101 cgcaccgaca gatcgaccgc gcggtcgcgt gtggcgaagc ggcttaccag ggtcagcgcc
     8161 tggttgcctg cggattccgg ccattcggcc tgcacgatac ccgcctggta cttattgtcc
     8221 acggtgagca ccgtcttggc accctctgcg gtccacgtgc tgccgaggtt cttctggtag
     8281 tcggtatcgt tcgtgagcgg caccggcaac catatccgcg ccgcacccgc cggtttgaac
     8341 acctccgccc gggtggtcac ctcgaatacc cgccatgggc cggatgccgt ggcaatgcgc
     8401 ggcaggccgg aaaccgccac agcggcgggc actgcagcag ttgtcttgat gaacgtgcgt
     8461 ctatccatgg atagctctcc ttaaggcgcg acgacagaaa ccttccggcc aaggacccgg
     8521 gtcgaggaca ccggactgaa ttgcgaatgc cgcggacgac agtggtaaaa atctgaggga
     8581 acgtgcgtga ggggagaaaa acgatgaggg acggcgcagt tacacgcgct gtcatccggg
     8641 ctgcgcgagc gcatggccgt tccccgtccg ccgcccgtgc acgcatagcc tatgctgact
     8701 gacaaggcga cagagctgcg tgacgtcagg cgcgcaacga taaacacggt gccaatctac
     8761 cgcttagtgg aatggcggac aagccgctcc accccaataa cactggtcgg gacaaggtca
     8821 ttccactccg cgtttcgaaa aatccgctat ccggaacccg agcagccaca acgccgcaaa
     8881 ataggttgcc gcccccagaa taatcacccc gaccagattc ccggcgcgcg tgccagcacc
     8941 cacggcgagc catgaagcat cggtgccgga gacaagccac agcaccgagg ccatggcgta
     9001 gacagcgatg acgagcttca tcaggaacac cggccagccc ggccgtggcg catagatgtt
     9061 cctgcggagc agacctcgca gcagcagcgc tgcattgacg cacgccccga gaccgattgc
     9121 gagcgcgagt cccgcgtgct tgaacggcca gatgaaggcg aaattcatga gctgggtaag
     9181 cgcgagcgtc acaatggcca tcctgaccgg cgtccggata tcctggcgcg cataaaagcc
     9241 gggcgcgagc accttcacca tgacgagccc gatcaatccc acgctgtagg cgatcaccgc
     9301 gcggcgcgtg gcgaagacat cctcggcgct gaacgcgccg taatggaaca aggtggccac
     9361 cagcggcacc gcgagtaacg cgagcgccac ggcggcgggc gcggcgagca gcaaagtcag
     9421 ccgcaacccc cagtcgagca gctcggaata ctgatccggc gagcgctccg cgtgatacct
     9481 ggacaggctg ggcagcagta tcgtgcccag cgccacgccg agcagcccga tcgggaattc
     9541 catcagccgg tcggcgtaat agagccagga gacgctgccg gtgacgagaa aagaggcgaa
     9601 gatcacgttg attaccaggc tcacctggct gatcgagacg ccgaacaccg ccggccccat
     9661 ctgccgcaga acgcgccaga cgccctcgtc cttgagatcg agcctgaagc gcggcagcag
     9721 cccgaggcgg gcgagaaacg ggatctgcaa cgcgagttgc gcgatgccgc ccgcgaacac
     9781 cgcccaggca agcgccttca cgggggggtc gaagtacggc gcgccccaca acgcaaagcc
     9841 gataaacgac aggttgagca gcgtcggcgt gaccgccggc accgagaagc ggctgtaagt
     9901 attgaggatg ccgccggcga gcgcggtgag ggagatgaag aaaatgtacg ggaaagtgac
     9961 gcgcaaaaga cccacggtga gatcgaactt gcccggcgtc gccgtgaagc ccggcgcggt
    10021 gacgtagatg atgagcggcg cggccagcac gccgatcaac gtcacgacga aaagcgccgc
    10081 ggcgagcgag gcggcgacgt gatccaccag cagccgggtc tcctgcccgc cgcggcggct
    10141 cttgtactcg gagaggatag gaacgaaagc ctgggaaaac gccccttccg cgaacagccg
    10201 ccgcagcagg ttggggagct tgaaggcgac gaagaaagcg tcggtcgcaa gaccggcgcc
    10261 aaaaacgcgg gcgatgacga ggtcgcggac gaagccgagg atgcgcgaga tgagcgtcat
    10321 gctgctcacc gtcgccaggg tcttgagcaa gttcatacga ccacgcttcg gcacacgttg
    10381 caaaaacacc agaacatcag taagatggcg ccctttttcc gattcagtcc cggagtttac
    10441 cgcacatggc caatatcgca tcggcgcgaa agcgcgcgcg acaggcggaa aagcgacgcc
    10501 tgcacaatgc ggcactgcgc tcggaattgc gcagcgcgat ccgcaacgtg aggaaagcga
    10561 tcgccgcggg cgacaagaag gcggcgcgaa ccgtgttcga acgcgcagcg ggcgtgatcg
    10621 actccatcgc cgacaagcgc atcatccaca agaatgccgc ggcccgacac aagagccgcc
    10681 tcgcagccgg tatcaaggcg atgacctaag gcagcctccg aaaggctgtc ggcaagtaac
    10741 cggcgtcgtc gcgtcagctg ccttaattaa gaataaccac atccccgctt atcaagcgtg
    10801 ggctcagaat ttgcgccgac aagtttctcc ggcctgctgg tccgtccccg tatcgttgtt
    10861 tctatttagg aagtcgccgg cccagaggcg acttcctccg ggcgtttctc cttgcgtccg
    10921 gggcgcatct tcagctcgtt gtcgcgggca aacccgagca ggaaccggaa cgcgtccggg
    10981 tcgagctcct cgagcctcaa gtccaccacc acgcagcgca caccggccgc catgatgggt
    11041 ttgaggtacg gggaatagga aaggtgccgg tcctgaccga acaccgacat catcccgcac
    11101 agccgctccg cccagtcgct gggtcggaag ctatggcccg attccgtgat gccctggatg
    11161 acgatttcgg gagagccggg gaacatggcg atatggtgtc gtttgcgagc gcaatcttac
    11221 tatacgcacg ggccggaatc cgatgacaca cgcatgacaa cggggtgctc gcagcaacga
    11281 ggattccgct tgcgcccagg gggttgatgg ggtaacaata tgagcattcc gcacggcggc
    11341 ttccggccgc cgtgcttgtt ttctgggttt ggaaccgtat gacgcacgtc atggacacgt
    11401 atgcgcggct gcccgtcgcg ttcgagcgcg gcgagggctc atggctgtgg gacgcgagcg
    11461 gcaagcgtta tctcgacgcg gtggccggcg tcgcggtgtg cgggctcggt tacggccatc
    11521 ccaagtacgt ggccgccctg cgcgagcagg tgggcaagct cgtccacact tccaacctct
    11581 accggatcac gctgcaggaa aagctcgccg accggctcgc cgcgatctcg ggcatggaca
    11641 acgcgttctt ctgcaactcc ggctgcgagg cgaacgaagc cgcgatcaag ctcgcgcggc
    11701 tctacggcca caagaagaac atcgaggccc cggccatcgt ggtgatggag aaggcatttc
    11761 atggccgcac catcgccacg ctctccgcca ccggcagccg caaggtgcag gccgggttcg
    11821 agccgctggt cgccggtttc gtgcgcgtgc cgttcgacga tctcgaggcg gtgaagcggg
    11881 tggcggaaaa caaccgcagc gtggtggcgg tgctggtcga gctgcttcag ggcgaaggcg
    11941 gggtcaacgt ctgccatgat gactatctca acggcctgcg tgaaatctgc gacgccaacg
    12001 gctggctcct catgctggat gaagtgcaga gcggcatggg gcgcaccggc aaatggttcg
    12061 cgttccagca ttcgggcgtt gcgcccgacg tgatgccgct cgccaagggc ctgggcaacg
    12121 gcatgccgat cggcgcctgc ctcgccgccg gcgccgccgc gcagctgttc aagccgggca
    12181 gccacggctc gaccttcggc ggcaatcccc tcgcctgcgc cgcggcgctt gccacgctcg
    12241 acatcatcga gcaagagcgg ctgatggaga acgccgtcag aatcggcgac ttcatccggg
    12301 gcgagctcgc aaggcggctc ggcgggctga agggcgtcaa ggagatccgc ggcaagggtc
    12361 tgatgatcgg cgttgagctc gaattcccct gcggcgagct ggtgcagcag gcgctcgagc
    12421 gcggggcgct catcaacgtc acggtggaca atgtggtccg gctgctgccg ccgctcgtct
    12481 tcaagcgcga agaggccgag ttgctgctgg cgaccctcgt cccgctggtc accgagttcc
    12541 tcgacaggca gagcgcgccg cgggccgccg cacagcctgc ctgaacgcgc cgctcaaccg
    12601 gagtgacgca gcatggccgt ccggcactat cttcagttca gggacttctc ccgcgaggag
    12661 tacgagtacc tgttcgagcg cacgcgctgg atcaaggaca tgttcaagcg ctacgtgccg
    12721 taccagccgc tgcgcgaccg cacgctcgcc atggtgttcg agaagcattc cacccgcacg
    12781 cgggtctcgt tcgaggccgg catgcaccag ctcggcggca cggtgatcac gctcatgacg
    12841 cgcgacaccc agatggggcg cggcgagccg atggaggacg tggcgcgcgt cctcacccgc
    12901 atggtggaca tcgtcatgat ccgcacgttc gagcaggcga tcatcgagcg cttcgccgag
    12961 cactcgcggg tgcccgtggt gaacgggctt accaacgaat accacccgtg ccagatcctc
    13021 ggcgacctct acacctacct cgagcaccgc ggctcgatcc gcggcaggac ggtcgcctgg
    13081 atcggcgatt ccaacaacgt ctgcaacacc tggctgcagg cggccgaggt gttcgacttc
    13141 aacgtgcacg tctccacccc gcccggctac gaggtggagc ccgagcgcgc ggggctctac
    13201 ggcaccgacc actacgaggt attcgaggac ccgatgaaag cggcgaaggg cgcggacctc
    13261 atcaccaccg acgtgtggac cagtatgggc gatgagtccg aggacgcgga gcgcaagcgc
    13321 gacttcgagg cgtggcgggt ggacaaggag atgatgcggg cggcgaagaa ggacgcgctg
    13381 ttcatgcact gcctgccggc gcaccggggg gaggaagtgg ccgccgaggt gatcgacggc
    13441 ccgcagagcg tggtctggga cgaggccgaa aatcgattgc acactcagaa agccctaatg
    13501 gagtatttac tcctcggaag gatcaaaacc cagggcgacc tctggtcaac ttagatcggt
    13561 gaaccgtgaa cagtgaacgg tgaacggtaa aatctagcgg cccttacacg attgccgttc
    13621 actattcacc gttcaccatt cacgaattga aaatggacaa agtcaagaaa gtggtgctcg
    13681 cctactccgg cgggcttgac acctcggtga tcctgaagtg gctgcaggac acctacgact
    13741 gcgaggtcgt gaccttcaca gccgacatcg gccagggcgg ggaactggag cccgcgcgga
    13801 gaaaagccag gaagcttggc gtgaagcgga tcttcatcga ggatctgcgc gaggaattcg
    13861 cgcgcgactt cgtgttcccg atgttccgcg ccaatgccgt ctacgagggc gaatacctgc
    13921 tcggcacctc gatcgcgcgg cctctgatcg ccaagcatct ggtggaaatc gcgaaaaaga
    13981 ccggggcgga cgcgatcgcc cacggggcca ccggcaaggg caacgaccag gtgcgcttcg
    14041 agctcggcgc ctacgcgctg atgccggacg tcaggatcat cgcgccgtgg cgcgagtggg
    14101 acctcacctc gcgcgagaag ctgctggagt atgccgaagc gcacggcatc ccggtcgagg
    14161 gaaaaaagaa gcaaggcggc gcgccctatt ccatggacgc caacctgctc catatctcct
    14221 acgagggcgg catcctggaa gacccggact acgagcccga ggaatccatg tggcgcatca
    14281 cggtcccgcc cgagaa
//
LOCUS       DBNGWZ010000205         3328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_272976, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000205 DBNGWZ010000000
VERSION     DBNGWZ010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_272976"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="AC16CX_06100"
     CDS             complement(<1..1157)
                     /locus_tag="AC16CX_06100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3012121.1"
                     /translation="MKHAPGDDNYSELREGVRALCRQFDSAYWQKVDEARGYPEAFVE
                     ALTKGGWLSALIPEEYGGSGLSMTEASIIMEEINRSGGNSGACHGQMYNMGTLLRHGS
                     EAQKRQYLPKIAGGELRLQSMGVTEPTAGTDTTKIKTTAVRKGDRYVVNGQKVWISRI
                     QHSDLMILLARTTPLTEVKKKSEGMSIFMVDLRSARKKGLTVKPIRNLANHETNELFF
                     DNLEIPAENLIGEEGRGFQYIFDGLNAERILIAAECIGDGYWFTDRATQYAKERVVFD
                     RPIGKNQGIQFPIARAYVNVQAADLMRYKSAALYDAHKPCGAEANMAKLLAADASWEA
                     ANVCVQTHGGFGFASEYDVERKFRETRLYQVAPISTNMILSYIGEHVLGMPR"
     gene            1252..1695
                     /locus_tag="AC16CX_06105"
     CDS             1252..1695
                     /locus_tag="AC16CX_06105"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3012122.1"
                     /translation="MYITNSKRLTLAGAKKMMETVMSNAEKAGHAVAVAIVDAGGHPI
                     LIQRMDGGRFHTVHSSTTKAVCAASNKRPTTSKGAAGQSLDMMHALGLALAAGAERWT
                     AMEGGYPVIFDGECVGGIGVSGATWELDDRLAREAVEAIGASYKI"
     gene            1803..2816
                     /locus_tag="AC16CX_06110"
     CDS             1803..2816
                     /locus_tag="AC16CX_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3012123.1"
                     /translation="MPDKPLRVACIGMGWWSDVLADAMKRSSRFEIAACYTRSEEKRQ
                     AFAKKYDCKAAPSYEAILGDRSIAAVINTTPNNVHLETTRAAARAGKHVFLDKPIANT
                     VADGCAITEACRKAGVVLALGYQRRKESHFRWVRKQIDDGVFGRLVNAEANISRDRLG
                     KIDLSSWRYTAEGMPGGVMLQIGIHYTDVLEYLMGPIKAVNGRFAQLVLPGNNPDVAS
                     LVLEHENGALSTLNASYASASEYYVMNIYGKEASAYYDLHQGLRFLKRGNDRSEPVPC
                     EKNDTIREELEEFAGAVRGKGKPEMDGEASTRSLAVLRAGIKSSREGRRVEVAEILSD
                     PSA"
     gene            2880..>3328
                     /locus_tag="AC16CX_06115"
     CDS             2880..>3328
                     /locus_tag="AC16CX_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012124.1"
                     /translation="MRNVGQKNEHQSRATSHESRSPASWPDEIHRILTGNGVRQVAYV
                     PDSGHARLINLVRANKSMRAIPLTTEEEGVALAVGAWLGGEKSVLLLQSSGVGNCINM
                     LGMVQECRVPLLMLVTMRGQWGEFNPWQVPMSQATAPSLAAMGVVVQA"
BASE COUNT          640 a         1027 c         1076 g          585 t
ORIGIN      
        1 cggggcatgc caagaacatg ttcccctata tacgacaaga tcatgttggt ggagatcggc
       61 gcgacctggt agagccgcgt ctcgcggaac ttgcgctcga cgtcgtactc ggaggcgaag
      121 ccgaacccgc cgtgggtctg cacgcagacg ttcgccgcct cccacgaggc atcggcggcc
      181 agcagcttcg ccatgttcgc ttccgcgccg cacggcttgt gggcgtcgta cagcgccgcc
      241 gacttgtaac gcatcaggtc cgccgcctgc acgttgacat aggcgcgcgc gatcgggaac
      301 tggatgccct ggttcttgcc gatggggcgg tcgaacacga cgcgctcttt cgcgtactgc
      361 gtcgcccggt ccgtgaacca gtagccgtcg ccgatgcact cggcggcgat caggatgcgc
      421 tcggcgttga gcccgtcgaa gatgtactgg aagcccctgc cctcctcgcc gatcaggttc
      481 tccgccggga tctcgagatt gtcgaagaac agctcgttgg tctcgtggtt cgcgaggttg
      541 cggatcggct tgacggtgag cccctttttc ctcgcgctgc gcaggtccac catgaagatg
      601 gacatgcctt ccgatttctt cttgacctcc gtcagcgggg tggtgcgggc gagcaggatc
      661 atgaggtccg aatgctggat gcgcgagatc cacaccttct gcccgttcac cacgtaacgg
      721 tcgcccttcc tcaccgccgt ggtcttgatc ttggtggtgt cggtgccggc ggtgggttcg
      781 gtcacaccca tggactgaag ccgcagctcg ccgcccgcga tcttcggcag atactggcgc
      841 ttttgggcct ccgagccgtg gcgaagcagc gtgcccatgt tgtacatctg gccatggcag
      901 gcgccggagt tgccgcccga gcggttgatt tcctccatga tgatggaggc ctccgtcatg
      961 gagagccccg acccgccgta ctcttcgggg atcagggctg agagccagcc gcccttggtg
     1021 agcgcctcga cgaacgcctc cgggtagccg cgcgcctcgt ccaccttctg ccagtatgcg
     1081 ctgtcgaact gccggcacag ggcgcgcacg ccttcgcgca gttcactgta gttatcgtcg
     1141 ccgggagcgt gcttcattga cgtgccgcgg gaatgctgga aaatacgggc gattatagat
     1201 ggaaacggcc tcgggccgtc tccataattg caagattcga tagggggacc aatgtacatc
     1261 acgaattcca agcgccttac gctcgccggg gccaagaaga tgatggaaac cgtcatgtcc
     1321 aatgccgaaa aagccggtca tgcggtggcg gtcgcgatcg tggacgctgg cgggcatccc
     1381 atactcatcc agcgcatgga cggcgggcgc tttcacaccg tgcactcttc caccaccaag
     1441 gcggtgtgcg cagcctcgaa caagcggcct acgacgtcca agggcgcggc aggacagtcg
     1501 ctcgacatga tgcacgccct cgggctcgcg ctcgcggcgg gagctgaacg gtggacggcg
     1561 atggaaggcg gttatccggt catcttcgat ggcgagtgcg tgggcggcat cggcgtttcc
     1621 ggcgccacct gggaactcga cgaccggctc gcgcgcgagg cggtggaggc gatcggcgcg
     1681 agctacaaga tctgaaggat gagggatgac ccccgcagtg cgggaagtcg aggccaggtc
     1741 atccttcatc cttccgcctt catccttggt ccagcagatc aataacgata gaagaacagt
     1801 caatgcctga taaaccacta cgggtggcat gcatcggaat gggctggtgg tcggacgtgc
     1861 tggccgatgc catgaagcgc tcgagccggt tcgagatcgc cgcttgctac acgcgctcgg
     1921 aggaaaagcg ccaggcgttc gcgaagaaat acgactgcaa ggcggcgccg agctacgagg
     1981 cgattctggg agaccgaagc atcgctgccg tcatcaacac gacgccgaac aacgtgcacc
     2041 tggagaccac gcgcgccgcc gcccgggcgg gcaagcacgt gtttctcgac aagccgatcg
     2101 ccaatactgt cgcggacgga tgcgctatca cggaagcctg ccgcaaggcg ggtgtcgtgt
     2161 tggcgctggg ttaccaacgg cgcaaggaaa gccatttccg ctgggtccgc aagcaaatcg
     2221 acgacggcgt cttcggcagg cttgtcaatg cagaggccaa catcagccgc gaccggctgg
     2281 gcaagatcga cttgagctcc tggcgctaca cggcggaagg catgcccggc ggcgtcatgc
     2341 ttcagattgg tatccactac actgacgtgc tcgaatacct catgggaccg atcaaggccg
     2401 tgaacggccg gttcgcccag ctcgtgctgc ccggcaacaa cccggacgtc gcgagcctcg
     2461 tgctggagca cgagaacggc gcgctttcga cgctcaacgc aagctatgca tcggcttcgg
     2521 agtattacgt gatgaacatc tatggcaagg aggcaagcgc gtactacgac ctgcaccagg
     2581 ggctgcgctt tctcaagcgc ggcaatgacc ggtcggagcc ggtgccctgc gagaagaacg
     2641 atacgatacg cgaagagctg gaggaattcg cgggcgccgt gcgcggcaag ggcaagcccg
     2701 agatggatgg cgaagcctcg acccggtcgc tcgccgtgct gcgtgccgga atcaagtcct
     2761 cgcgggaagg ccgccgcgtc gaggttgccg agatactttc ggaccccagc gcataaaata
     2821 gacgccaaga ccgccaagaa aaacacacgg aaatccgaat aatgaaggac tcaaggaaaa
     2881 tgcggaacgt gggacaaaaa aacgagcacc agtcacgagc caccagtcac gagtcacgct
     2941 cccccgcctc gtggcccgac gagatccacc gcatccttac cgggaacggc gtccggcaag
     3001 tcgcgtacgt tccggattcc gggcatgccc ggctgatcaa tcttgtgcgc gcaaataaga
     3061 gcatgcgcgc aatcccgctg accacggaag aggaaggcgt ggcgctcgcc gtcggcgcct
     3121 ggctcggcgg cgagaagagc gtgctgctgc tgcagtcgag cggcgtgggc aactgcatca
     3181 acatgctcgg catggtccag gaatgccgcg tgcctctgct catgctcgtc accatgcgcg
     3241 gccagtgggg tgaattcaac ccgtggcagg tgccgatgtc gcaggccact gccccgtcgc
     3301 ttgcggcaat gggtgtcgtt gtgcaggc
//
LOCUS       DBNGWZ010000206         4039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2288821, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000206 DBNGWZ010000000
VERSION     DBNGWZ010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2288821"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..1618
                     /locus_tag="AC16CX_06120"
     CDS             239..1618
                     /locus_tag="AC16CX_06120"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3012125.1"
                     /translation="MDSMIERLAEYAAGLRYEDLPQEAVHECKRKLIDTLGCLAGGFD
                     AGPSVIARKMARQSRGTPPARILGTQERSSPELAAFANGVALRYLDYNDAHFMKSSGH
                     PSDTFAAVLAAADAAQADGRGVVTAAAAAYEAYCGISDAVKREQGWDHSVQAVIGSAV
                     GAARLFGLGREAMGNAISLAVIPNMALEQTRTGELAMWKGCAGANAARNGVFAAQLAR
                     EGLTGPQQAIEGKWGLWHALSRFEWQPFGGRGGPYRITQTHIKYYPVVVHAQSPVTAA
                     LELHGTVDSKDIEAVAIVTYWVAERYVERGNPLWRPATRETADHSIPYCVAAALLDGG
                     ISEASFSAKRIRDPRIAALLEHTTIREDPSYTRLHPHEWPCRIELTLRGGKQKAAEAR
                     YFKGHAKRPLSDAELEEKFRTLAEPALGKKRADAVVATAWKLEQLADVRDLLKLLRFG
                     GKKRKRTKA"
     gene            complement(1734..2696)
                     /locus_tag="AC16CX_06125"
     CDS             complement(1734..2696)
                     /locus_tag="AC16CX_06125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012126.1"
                     /translation="MHRLRVLVLLSLFIPLAVSAAERYPFKAVRIVVGFPPGGSDDTH
                     ARLLAQKMTEALGRQLVVDNRAGAGGIIGQDSVAKAAPDGYTLLFAGPAIVIAPNLYP
                     KMPYDLMRDLTPVSRVATFMAVLVVHPSVPAKSVKELIALARSNPGKLNFASSGVGAV
                     PHLSGELFKQMAKVDMAHVAYKGGAPAYVGLIGGQVDMYFGLMGSAMSYIVAGKVRPL
                     AVTSTARSKALPDVPTMAEAGLPSYNLTGWYALMAPSATPRDIISQLNAAVVNAVGSP
                     DLQERLLKLGSEPAISTPEQMLQRLREDMNKFSQLIKTAGVRLD"
     gene            complement(3072..>4039)
                     /locus_tag="AC16CX_06130"
     CDS             complement(3072..>4039)
                     /locus_tag="AC16CX_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012127.1"
                     /translation="SSFILGSAAALAVALICAAASAQPYPVRPIRLIVPIAPGGGLDT
                     IARAVGQKLTDNFGQTIVVDNRPGASGAIAVELAKLAPADGYTILMISASQVIRPLLY
                     NVPYDLLRDFAPLTQLSAQSYVLMASNALPVRSVRELIAHARANPGKLNYASVGQGSQ
                     IHLTTELFRTLTRIQVVHVPYKGIAAAYPDLMAGIIHFSFGGIISAQTHIKAQRLRAL
                     AVSGPRRISALPDLPTIAEAGVPGFAVTQWYALLAPAGTQRQLVEFLNKEINKALQQP
                     EVAGRIAAEGSEAVGTSSAQLATIIKAERAKWARVIKEAGIKGEE"
BASE COUNT          767 a         1253 c         1316 g          703 t
ORIGIN      
        1 tgatcgcatg agtagtcgat cctggttacc gcggagccga caggctgaaa tggagtattt
       61 attgatcggg attctgaatg aggtcaaccg cgctccgtgc ttccgcctca cgctgtaaat
      121 cgggaagaac tgagtcagca aaaaatggcg tcggggtgga gtagcattgg tgaccgcgat
      181 taaaattggt ccacaggaga aattgctgca agaccgtcca ctacagacgc cattttttat
      241 ggactcgatg atcgaacggc tggcggaata cgcggcaggc ctgcgctatg aggacctgcc
      301 gcaagaggcc gtccacgagt gcaagcgcaa gctcatcgac acgctcggtt gcctcgccgg
      361 cgggttcgat gccgggccgt ccgtcatcgc gcgcaagatg gcgcggcaaa gccgcggcac
      421 gccgcccgcg cgcatcctcg gcacgcagga gcgctcctcg cccgagctgg cggctttcgc
      481 caacggggtc gcgctgcgct atctcgacta caacgacgcc catttcatga agtcgagcgg
      541 gcatccgagc gacaccttcg ccgccgtgct cgccgcggcg gacgcggcgc aggccgacgg
      601 ccgcggcgtc gtgaccgccg cggctgccgc ctacgaggcc tattgcggca tctccgatgc
      661 agtcaagcgg gagcaggggt gggaccattc ggtgcaggcc gtcatcggaa gcgcggtggg
      721 cgcggccagg ctcttcgggc tcggccgcga ggcgatgggc aacgccatct cgcttgcggt
      781 gatcccgaac atggcgctcg agcagacgcg caccggggag ctggccatgt ggaagggctg
      841 cgcgggcgcc aacgcggcgc gcaacggcgt gttcgcggcg cagctcgcac gcgaagggct
      901 gaccggaccg cagcaggcga tcgaaggcaa atgggggctg tggcatgcgc tcagccgctt
      961 cgagtggcag cccttcggcg ggcgcggcgg gccgtaccgg atcacgcaga cccacatcaa
     1021 gtactacccg gtcgtcgtgc acgcgcagtc gccggtcacg gccgcgctgg agctgcacgg
     1081 taccgtcgat tcgaaggaca tcgaagcggt cgcgatcgtc acctactggg tggcggagcg
     1141 ctacgtggag cgggggaacc cgctatggcg cccggcgacg cgcgagaccg cggatcacag
     1201 cattccctac tgcgtcgccg cggcgctgct cgacggcggc atcagcgaag cgagcttttc
     1261 cgctaagcgc atccgcgatc cgcgcatcgc cgccttgctc gagcacacca cgatacgaga
     1321 ggacccctca tacacccgcc tgcaccctca cgagtggccg tgccgtatcg agctcaccct
     1381 gcgcggcggc aagcagaaag ccgcggaggc gcgctatttc aaggggcacg cgaagcggcc
     1441 gctgagcgac gcggagctgg aggaaaaatt ccgcaccctc gcggaaccgg cgctcgggaa
     1501 gaagcgggcg gacgccgtag tggcgacggc ctggaagctg gagcagcttg cggacgtgcg
     1561 cgacctgctg aagctattga ggttcggcgg gaagaaacgt aagcgaacga aggcgtgaac
     1621 gagtccggga taggtgtttg cctttacttg tactggttca gacttgatct gacacactgt
     1681 gtgggaagga tcaagatcgg ctaactagcg ccgcggctct agccaatagt tcactaatcc
     1741 agcctcactc cggcagtctt gatgagctgc gagaatttgt tcatgtcttc ccgcaggcgt
     1801 tgcaacatct gctcgggagt agaaatcgcc ggctccgagc cgagcttgag caggcgctcc
     1861 tgcaaatccg gtgaacccac cgcgttgacg accgccgcgt taagctgcga gatgatgtcg
     1921 cgtggcgttg ctgacggcgc catcagcgca taccagccgg taaggttgta cgagggcagt
     1981 cccgcctcgg ccatggtcgg tacgtccggt agcgctttgg agcgagcggt gctagtcaca
     2041 gccagcgggc gcaccttgcc tgctacgata taactcatgg cgctgcccat gaggccgaaa
     2101 tacatgtcga cctgtccgcc gatcagaccg acgtacgcgg gtgcgccgcc cttgtacgca
     2161 acgtgcgcca tgtcgacttt cgccatctgc ttgaacagct cgccacttag atgcggcaca
     2221 gcgccgactc ccgaggaggc gaaattgagc ttacccggat tggatctcgc cagcgcgatc
     2281 agttccttca ccgacttggc cggcacggag gggtgcacaa ctaagacggc catgaacgtg
     2341 gccacgcgcg ataccggcgt aagatcgcgc atcaaatcgt aaggcatttt cgggtagagg
     2401 ttcggcgcaa tgactatcgc gggaccggca aatagcagtg tatagccgtc cggtgccgct
     2461 ttggcgaccg agtcctgccc aatgatgccg cccgcgccag cgcgattgtc cacgaccagc
     2521 tgtcgcccaa gcgcttcggt cattttctgc gccagcaggc gagcatgggt gtcgtcagaa
     2581 cctccgggcg gaaaccccac cacgatcctg acggccttga acggatagcg ctccgcggcg
     2641 gacacagcaa gagggataaa cagagagagc aggacgagaa cccgcaacct atgcatagct
     2701 ggcctccgat gttgctatgc cggcctcatg accagcattc attccagtga attcgcgtgc
     2761 tcacgcgccc acattcgagt aacacggggg gctacggata catcgccatc gatattacgc
     2821 cgatgcagta gctcggaaca agaatgaata ccgaatcagt gtccattctt tgtgtccgat
     2881 tcgctccctg atcgggactc gaaccaagaa cccggataac agccagaggg aacgaccgat
     2941 ttaactcgat ttgagtcgct cgtgaggatc caggattcgt ttcgtcgttg ccgattaggc
     3001 atcgttcccg tctgacacag tctgtcagat caagtctgac ctatcacact ttactcatca
     3061 cccatcaccc atcactcttc gcctttgatt cccgcctcct tgatcacgcg cgcccacttg
     3121 gcgcgctcgg ccttgatgat ggtcgccagt tgcgcggagc tggtgccgac cgcttcggag
     3181 ccttccgccg cgatgcggcc ggccacctcg ggctgctgca gcgccttgtt gatctccttg
     3241 ttgaggaact cgacaagctg gcgctgcgtc cccgccggcg cgagcagggc ataccactgg
     3301 gtgaccgcaa agccgggcac ccccgcttca gcaattgtcg gcaggtcggg cagcgcgctg
     3361 atgcgtctgg gcccggagac cgccaacgcc cgcagccgct gagccttgat gtgggtctgg
     3421 gcgctgatga tgcccccgaa ggaaaaatga atgatgccgg ccatgagatc cgggtaggcg
     3481 gcggcgatgc ccttgtacgg gacatggacg acctggatgc gggtgagcgt ccggaacagc
     3541 tcggtcgtga ggtggatctg gctgccctgt cccacggagg cgtagttgag cttgccggga
     3601 ttcgccctgg cgtgcgcgat gagctcccga accgaacgca cgggaagcgc attgctcgcc
     3661 atgagcacgt aggactgcgc gctcaactgc gtgagcgggg cgaaatcgcg caggaggtcg
     3721 tacggtacgt tgtagagcag cgggcgtatc acctggctcg cgctgatcat gaggatcgtg
     3781 tagccgtccg cgggcgcgag cttggcgagc tcgacggcaa tcgcgccgct cgccccgggg
     3841 cggttgtcca ccacgatggt ctggccgaag ttgtcggtca gcttctgtcc gaccgcgcgt
     3901 gcgattgtgt cgaggccgcc tcccggggcg atcggcacga tgagccggat cggccgtacc
     3961 ggatacggct gcgcgctggc cgcggcgcag atcaacgcca cggcgagcgc agcagccgat
     4021 ccaaggatga aggaagaag
//
LOCUS       DBNGWZ010000207         5424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1028961, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000207 DBNGWZ010000000
VERSION     DBNGWZ010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1028961"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /gene="accB"
                     /locus_tag="AC16CX_06135"
     CDS             <1..278
                     /gene="accB"
                     /locus_tag="AC16CX_06135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3012128.1"
                     /translation="PAAPAESEAAAAPEGHVIRSPMVGTFYRSSAPGAKSFVEVGQAV
                     KAGDTLCIIEAMKLLNEIEADRDGVVKTILVENGQPVEYGEPLFILG"
     gene            376..1731
                     /gene="accC"
                     /locus_tag="AC16CX_06140"
     CDS             376..1731
                     /gene="accC"
                     /locus_tag="AC16CX_06140"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3012129.1"
                     /translation="MFEKILIANRGEVALRIQRACRELGIKTVVVHSEADRDAKYVKL
                     ADESVCIGPPPSSSSYLNIPAIISAAEVTDAEAVHPGWGFLAENADFAERVAQSGFVF
                     IGPKAETIRMLGDKVNAKAAMKKAGLPVVSGVDEPLPQEPNDVLKIARSIGYPVIVKS
                     AGGGGGRGMRVVHTEAALLTAVQMTRSEAQAAFGNPAVYLEKYLENPRHIEFQVLTDR
                     FRNAIHLGERDCSMQRRHQKVIEEAPAQHLNSRSRLRIAERCAEACHKLGYEGAATFE
                     FLYENGEFYFIEINTRLQVEHPVTEMITGIDIVQQQIRIAAGEKLRIRQRDVQFRGHA
                     IECRINAEDPYRFTPSPGRITSYHMPGGPGIRVDSHAYNGYFMPPHYDSLLGKIIAYG
                     DTREQAIARLSIALSETVIEGIKTNIPLHQELLRDAAFLRGGTSIQYLEEKIATLARG
                     E"
     gene            1857..2750
                     /gene="prmA"
                     /locus_tag="AC16CX_06145"
     CDS             1857..2750
                     /gene="prmA"
                     /locus_tag="AC16CX_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3012130.1"
                     /translation="MAWLSISFEVAAADVEAVSAALLEAGAASVDVADADAGSPAEQP
                     IFAGSGNGTPHPWRRSLVSALVAEGTDATAFVSASCARAGIPPPRYRARPVAEQDWVR
                     ASRDQFAPIRVSPRLWIVPSWHEAPDPDAVNVLLDPGLAFGTGDHPSTRLALRWLERT
                     IRGGESVLDYGCGSGILAIAAMKLGAGRADGVDIDEQALLAARRNAMHNRVQAHFHSA
                     AGALRQPAQIVVANILAHPLIVLAPLLARLTEQHGRLALAGLLAEQAGEVRAAYESWF
                     DFEAPELDEGWALLSATRRPS"
     gene            2747..3580
                     /locus_tag="AC16CX_06150"
     CDS             2747..3580
                     /locus_tag="AC16CX_06150"
                     /inference="COORDINATES: protein motif:HMM:NF023334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HIR3012131.1"
                     /translation="MSLITKCTSCGTTFRVTPQQLQARNGQVRCGQCLAVFDGLKALI
                     TPPDTAQEAPLRRAGTGEAAGFELEPVEPAAVPAPPPAAALASEAAYEERDYGPPPEQ
                     LSLDEQLYPEVPRAAARWWAVGAALLVFVLIGQGAYFYRADLATQYPGLKPYLVQFCD
                     LLNCTVSPPQRPRQIAIEASDLQAIDPARPRIIQLTATLRNHAGHDLGYPALDLVLTN
                     TKEHTLARRIFLPGEYLERGRDVAAGIPANAEITIRLDLDTGDLNPAGFRLDLLPAPP
                     P"
     gene            3605..4714
                     /gene="gcvT"
                     /locus_tag="AC16CX_06155"
     CDS             3605..4714
                     /gene="gcvT"
                     /locus_tag="AC16CX_06155"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR3012132.1"
                     /translation="MSTTQAALRATPLHAVHRALGAKMVDFAGWEMPLAYGSQVEEHH
                     RVRRDAGVFDVSHMLAVDARGKGARAFLQRVLANDVAKLKAPGKALYSCLLNEAGGML
                     DDLIVYRLAEDFFRLVVNAATAEKDCDWLERQRARFTPPVAIEPRRDLAIIAVQGPRA
                     REKTWSALPGLRVATGALEYFQAVEVENMMVARTGYTGEDGFEIVLPAGRANAVWRAL
                     LERAVAPAGLGARDTLRLEAGMNLYGQDMDETVTPLESGLAWTVALGDGRDFIGRQAL
                     ESRPATRQLLGLVLTDGGVMRNHQKVRCGSGAGEITSGGYSPTLGRSIALARLPRAVA
                     AGDTVDVEVRGKWLHARVVKPPFVRHGKILISAEC"
     gene            4824..5213
                     /gene="gcvH"
                     /locus_tag="AC16CX_06160"
     CDS             4824..5213
                     /gene="gcvH"
                     /locus_tag="AC16CX_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR3012133.1"
                     /translation="MTTPENLKYTRSHEWVKAESDGTVTVGVTFHAQEQLGDVVFIQN
                     PVVGRKVKQGEECGVIESVKAAADIYAPVSGEVVAANQEVADKPERVNQDPYAAWMFR
                     IKPASPAELSGLLDAAAYRKIAESEKR"
BASE COUNT          937 a         1784 c         1815 g          888 t
ORIGIN      
        1 ccccggcggc accagccgag agcgaggccg cggctgcgcc cgaggggcat gtcatccgtt
       61 ccccgatggt cggcactttc taccgctcct ccgcccccgg ggcgaagtcc ttcgtcgagg
      121 tcgggcaggc ggtcaaggcg ggcgatacgt tgtgcatcat cgaggccatg aagctgctca
      181 acgagattga ggccgaccgc gacggcgtgg tgaagacgat cctcgtagag aacggccagc
      241 cggtggagta cggcgagccc cttttcatac ttgggtaatg ggtcatgggt aatgggtgat
      301 gcgtaagacg gcggtgccac aggcgcgggt tttcgcccat tacccattac gcatcaccca
      361 tcaccgctct tccagatgtt tgaaaaaatc ctgatcgcca accgcggaga ggtggcgctg
      421 cgcatccagc gcgcctgccg cgagctcggc atcaagacgg tggtggtgca ctcggaggcg
      481 gaccgcgacg ccaagtacgt caagcttgcg gacgaatcgg tttgcatcgg ccccccgcct
      541 tcgagctcga gctatctcaa catccccgcc atcatcagcg ccgcggaggt caccgatgcc
      601 gaggcggtgc acccgggctg gggctttctc gcggagaacg ccgattttgc cgagcgcgtc
      661 gcgcagagcg gtttcgtgtt catcggcccc aaagccgaaa ccatccgcat gctgggcgac
      721 aaggtgaacg ccaaggccgc catgaagaaa gcagggctgc cggtggtgtc tggcgtggat
      781 gaaccgctgc cgcaggaacc gaacgacgtg ctcaagatcg cacgctcgat cggctacccg
      841 gtgatcgtca agtcggcggg cgggggcggc gggcgaggca tgcgcgtggt gcacaccgag
      901 gcggcgctgc tgaccgcggt gcagatgacg cggagcgagg cgcaggctgc tttcggcaat
      961 cccgcggttt atctggagaa atacctggaa aatccgaggc acatcgagtt ccaggtgctc
     1021 accgaccggt tccgcaacgc gattcacctc ggcgagcgcg actgctcgat gcagcggcgg
     1081 caccagaagg tgatcgagga ggctcccgcg cagcacctca actcgcgcag ccgcctccgc
     1141 atcgccgagc gctgcgcgga agcctgccac aagctcggtt acgagggcgc cgctaccttc
     1201 gagttcctgt acgagaacgg cgagttctac ttcatcgaga tcaacacccg ccttcaagtc
     1261 gagcaccctg tgaccgagat gatcacgggc atcgacatcg ttcagcaaca gatccggatc
     1321 gcggcgggcg agaagctgcg cattcggcag cgcgacgtgc aatttcgcgg gcacgccatc
     1381 gagtgccgca tcaacgccga ggacccgtac cgcttcacgc cctcgcccgg gcgcatcacg
     1441 tcgtatcaca tgcccggcgg tcccggcatc cgcgtggatt cgcatgccta caatggttat
     1501 ttcatgccgc cgcattacga ttccctgctc ggcaagatca tcgcctatgg cgacacccgc
     1561 gagcaggcga tcgcgcgcct cagcatcgcg ctctcggaga ccgtgatcga gggcatcaag
     1621 accaatatcc cgctccacca ggagctgttg cgggacgcgg ccttcctgcg cggtggcacc
     1681 agcatccagt atctcgaaga gaaaatcgcc acgctcgccc gcggggagtg atcaagagta
     1741 gtgattaagt gactaggtaa ctgagtgatt gagcgggcag tgtggctgta acggtcagtt
     1801 gctgaatcgc ccaatcactc aatcacccaa tcacttagtc actcagtcac ccgctcatgg
     1861 cgtggctttc catctccttc gaggtcgcgg cggccgatgt cgaggcggtc tccgccgccc
     1921 tgctcgaagc cggggcggcg agcgtggacg tggcggatgc cgacgccggc tcgccggccg
     1981 agcagccgat atttgccgga tccgggaacg ggacgccgca tccctggcgg cgcagcctcg
     2041 tgagcgcgct ggtcgcggag ggcacggatg ccacggcgtt tgtctcggcg tcatgcgcgc
     2101 gcgccggcat cccgccgccg cggtatcgtg cgcgccctgt agccgagcag gactgggtgc
     2161 gcgcgagccg cgaccagttt gccccgatcc gggtttcgcc ccggctgtgg atcgtaccga
     2221 gctggcacga ggcgcccgat cccgacgcgg tcaacgtcct gctcgacccc gggctcgcgt
     2281 tcggcaccgg tgaccatccc agcacgcgcc tcgccctgcg ctggctcgag cgcacgatcc
     2341 gcggcggaga gtctgtgctc gattacggct gcggttccgg catactcgcg attgcggcga
     2401 tgaagcttgg ggcggggcgc gcggacggcg tggacatcga cgagcaggcg ctgcttgcgg
     2461 cgcgccgcaa tgctatgcac aatcgggtgc aggcgcattt tcacagcgct gccggcgcgc
     2521 tccggcagcc cgcgcagatc gtggtcgcca acatcctcgc ccatccactc attgtgctcg
     2581 cgccgctgct cgcgcggttg accgagcagc atgggcggct tgcgctcgcc gggctgctcg
     2641 cggaacaggc gggcgaagtg cgcgcggcgt acgagtcgtg gttcgatttc gaagcgcccg
     2701 agttggacga aggctgggcg cttctcagcg ccacaaggcg cccgtcatga gcctgatcac
     2761 gaaatgcact tcctgcggca cgacattccg cgtcaccccg cagcagttac aggcccgcaa
     2821 cgggcaggtg cggtgcggcc aatgcttggc ggtgtttgat ggcctgaagg cgctcatcac
     2881 gccgcccgac accgcgcagg aggcgccgct gcggagggcc ggcacgggcg aggccgctgg
     2941 attcgagctc gaaccggtgg aaccggcggc agtgcccgca ccgccacccg ccgcggcgct
     3001 cgcgtccgaa gccgcatacg aggaaaggga ttacggcccg ccgccggagc agctctcact
     3061 cgacgagcag ctctatcccg aggtgccgcg agccgctgca cgctggtggg cagtaggcgc
     3121 tgcgctgctg gtgttcgtcc tgatcggtca gggcgcctat ttctaccgcg ctgacctggc
     3181 aacgcagtat cccggcctga aaccctacct cgtccagttc tgcgacctgc tcaattgcac
     3241 ggtttcgccg ccccagcgtc ccagacagat cgcgatcgag gcttccgacc tgcaggcgat
     3301 cgatccggcg cgaccgcgga tcatccagtt gaccgccacg ctgcgcaatc atgccgggca
     3361 cgatctcggc tatccggcgc tcgatctcgt gctcaccaac accaaggagc acacactcgc
     3421 gcggcgcatc ttcctgccgg gggagtacct cgagcgcggg cgggatgtcg ccgcgggcat
     3481 cccggcgaac gcggagatca ccatccggct cgatctcgac accggcgacc tcaatcccgc
     3541 cggcttccgt ctcgacctgc tgccggcgcc gccaccctga ccgcgggcct catctttttt
     3601 tgcgatgagc acaacccagg cagcgcttcg cgccactcca ctccatgccg tccatcgcgc
     3661 gctcggcgcg aagatggtgg atttcgcggg gtgggagatg ccgctcgcgt acggctcgca
     3721 ggtcgaggag caccaccgcg tgcggcgcga tgccggcgta ttcgacgttt cccacatgct
     3781 ggcggtggac gcgcggggga agggcgcgcg cgcgttcctg cagcgcgtgc tggccaacga
     3841 cgtcgcgaaa ctgaaggccc cgggaaaggc gctttattcc tgcctgttga acgaagccgg
     3901 cggcatgctc gacgacctca tcgtgtatcg cctggccgag gacttcttcc gcctggtggt
     3961 gaacgcggct accgcggaga aggactgcga ctggctcgag cggcagcgcg cccgcttcac
     4021 gccaccggtt gcgatcgagc cgcgtcgcga tctcgccatc atcgccgtgc aaggcccgag
     4081 ggcgcgggag aaaacatgga gcgcactgcc cgggttgcgc gtcgcgaccg gtgcgctcga
     4141 gtatttccag gcggtggagg tggagaacat gatggtggcg cgcaccggct ataccggtga
     4201 ggacggcttc gagatcgtac tccccgccgg ccgcgccaac gcggtgtggc gcgcgctgct
     4261 ggagcgggcg gtcgcgcccg ccggcctcgg cgcgcgcgac acgctgcggc tcgaggcggg
     4321 catgaacctg tacggccagg acatggacga gaccgtgaca ccgctcgaat cggggcttgc
     4381 ctggacggtg gcgctcggag acgggcgcga tttcatcggc aggcaggcgc tcgagtcgag
     4441 acccgcaacc cggcagctgc tcggcctggt gctcaccgac gggggcgtga tgcgaaatca
     4501 ccagaaggtg cgctgcggct ccggcgcggg ggagatcacc agtggcggct attcgcccac
     4561 gctcggccgc tcgatcgcgc ttgcgcgcct gccgcgcgcc gttgcggccg gcgacacggt
     4621 cgatgtcgag gtgcgcggaa aatggctgca cgcaagagtg gtgaagccgc ctttcgtgcg
     4681 ccacggcaag atactgatca gtgccgagtg ctaagtgctg agtgaagtta cgctcgcgaa
     4741 agccagttga tccttaatgc tcaaagtcca atactcagca ctcagaactc agcattcaga
     4801 actcagcact ccccggaggg gccatgacga cgccggagaa cctgaaatac acccggtcgc
     4861 atgaatgggt aaaagcggag tccgacggaa cggtgacggt gggcgtcacg tttcatgcgc
     4921 aggagcagct gggcgacgtg gtgttcatcc agaacccggt agtcggccgc aaagtgaagc
     4981 agggcgagga atgcggcgtg atcgagtcag tcaaggcggc cgccgacatc tacgcgccgg
     5041 tgtccggcga ggtggtcgcc gccaaccagg aggtggcgga caagccggag cgcgtcaacc
     5101 aggatccgta cgctgcgtgg atgttccgca tcaagcccgc cagccccgcc gagctttcgg
     5161 gattgctgga cgctgcggcg taccggaaga tcgcggagag tgagaagcga tagcggtaag
     5221 cggtaagccg tgagccgtaa atattcgctt cgctgaccgc caaccgctca ctgcttacag
     5281 ctcaccgctc acgaatgtat gccgttcatc ccacacaccg agactgacgt tcgcgagatg
     5341 ctcgcggcga tcggggttgc ctcgatcgag gatctgttcg aggagatccc gcgcgagttg
     5401 cgcggcgccg cgttctccgg cctc
//
LOCUS       DBNGWZ010000208         2871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_273143, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000208 DBNGWZ010000000
VERSION     DBNGWZ010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_273143"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="AC16CX_06165"
     CDS             complement(<1..808)
                     /locus_tag="AC16CX_06165"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012134.1"
                     /translation="MTTKKKSSTRKTSRARKVMNYDLHNHVIPQTVVDAIRRNPERYD
                     TRIEDRNGKPYFDSHGRMTELLPEFCDPAAKIAWMDRVGMDVSVISVGPPIFFYWLKP
                     EAGIEIVRLANDGIAQMVAKYPDRFRGMAHLPMQEPDAAVAELERVVREYRFKAVELA
                     TSIEGVPLADMKFRKVLKAIEQLGLFIFTHPYQCLAQGGMDGYYLRNFVGFPLDTTLM
                     VAHLMFSGALDDLKKLRILLAHGGGFIPYQIGRFVHGHKVRPEPKVNNASS"
     gene            complement(1024..2016)
                     /locus_tag="AC16CX_06170"
     CDS             complement(1024..2016)
                     /locus_tag="AC16CX_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012135.1"
                     /translation="MRRSISVVLLSGLAGVAHTVATTAAQTDAAVRHYPAKPVRIIVP
                     QSPGASTDLTARLVAQKLSEAFKQPVVVDNRPGGSGITGAELVARATPDGYTLMVVAS
                     SFSINPAIQPKLPYDPIRDFTPVSQLSKFPNMLAAHPSVPVKTLQDVIALAKAKPGQL
                     NYASAGSGTGTHMSAELLKQMTRIDIVHIPYRGGGPAAIAAMGGQTQLIIGTSFGLLP
                     HVRAGKLKAIALTSARRSPAAPDIPTFAESGVPGYEHEPWNGMFGPARIPKPLLTRIN
                     GEVVRILHAPDVTKVFEHEGADVVGSTPEQYGAFLRAEIAKWTKVAKAAGIKPE"
     gene            complement(2085..>2871)
                     /locus_tag="AC16CX_06175"
     CDS             complement(2085..>2871)
                     /locus_tag="AC16CX_06175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012136.1"
                     /translation="LVVDNRGGGGGSVGADVVAKSAPDGYTLLLGNAGPITVNPSLRR
                     DLPYNPQRDLVPVTQIVAAPMVLVVHPSLPVRSVKELIAFAKARPGQLNYASAGIGNL
                     QHLGMELLQSQSGIRMNHVPYKGAAPAFLDLLSGRVELMFANIVGVLPHIWSGKVRPI
                     AVSAAKGSPVLPDVPGVAATLPQFDLDGWMGIFARAGTPPEVIAKLHKDFARVGENKE
                     IRENLAKRGAQVVGGGPEGLARIVREETALYANIIRSAKIAPE"
BASE COUNT          452 a          967 c          923 g          529 t
ORIGIN      
        1 gcgagctcgc gttgttgacc ttgggctcgg gccgcacctt gtgcccgtgc acgaagcgcc
       61 cgatctggta cgggatgaag ccgccgccgt gcgcgagcag gatgcgcagc ttcttcaggt
      121 cgtcgagcgc gccgctgaac atgaggtgcg ccaccatgag tgtcgtgtcg agcgggaaac
      181 cgacgaagtt gcgcaggtag tagccgtcca tcccgccctg cgcgaggcat tggtacgggt
      241 gcgtgaagat gaacagcccg agctgctcga tcgccttcag cacctttcgg aacttcatgt
      301 ccgcaagcgg cacgccctcg atcgaggtcg cgagctcgac cgccttgaac ctgtactccc
      361 tcaccacgcg ctccagctcg gcgaccgccg cgtcgggttc ctgcatcggc aggtgcgcca
      421 tgccgcggaa ccggtcgggg tacttcgcca ccatctgcgc gatgccgtcg ttcgccaggc
      481 gcacgatttc gatcccggct tcgggcttga gccagtagaa gaagatcggc ggcccgaccg
      541 agatcaccga cacgtccatc cccacgcggt ccatccacgc gatcttggca gcgggatcgc
      601 agaattcggg cagcagctcg gtcatgcgcc cgtggctgtc gaaatacggc ttgccgttcc
      661 ggtcctcgat gcgcgtgtcg taacgctccg ggttgcgccg gatggcgtcc accacggtct
      721 gcgggatgac gtggttgtgc aggtcgtagt tcatcacctt cctggcgcgg gacgtcttcc
      781 gggtgctgct tttcttcttg gttgtcatgc ttttcctttc atgaaaatcc gtgaatcgtg
      841 aagtcgggag atcgggagat cgaaagaccg ggagatcgag agatcgggag gtcgtgagag
      901 cgaaacagcg cgtaaagccc ttcccctctc ccgcactcac gctctcccgc cctcacgggt
      961 cttgtccccc tcccgcgctc acgctctccg ctctcacgtc tgttttcccc aacccttgcg
     1021 gattcattcg ggcttgatgc cagccgcctt cgcgaccttc gtccatttgg cgatctccgc
     1081 cctgaggaag gcgccatact gctccggcgt gctgcccacg acgtcggcgc cttcatgctc
     1141 gaatactttc gtcacgtcag gcgcgtgcag gatgcgcacg acctcgccgt tgattctcgt
     1201 gagcaggggc ttcggtatcc tggccggtcc gaacatgcca ttccagggct cgtgctcgta
     1261 gcccggcacg cccgactcgg caaaggtggg aatatccggc gcggctggcg accgcctggc
     1321 gctggtcagg gcgatcgcct tcagcttccc tgcccgcacg tgcggcagca ggccgaacga
     1381 ggtgccgatg atgagctgtg tctgcccgcc catcgccgcg atcgcggccg gaccgccgcc
     1441 cctgtacggt atgtgcacga tatcgatgcg ggtcatctgc ttcagcagct cggccgacat
     1501 gtgcgtgccg gtgcccgagc ccgccgatgc gtagttgagc tgccccggct tcgccttcgc
     1561 gagcgcgatc acgtcctgca gcgtcttcac cggcaccgag ggatgggccg ccagcatgtt
     1621 cggaaacttc gagagctgcg aaacgggcgt gaaatcccgg atcggatcgt agggcagctt
     1681 gggctggatg gcgggattta tcgagaacga cgacgcgacc accatgaggg tatatccatc
     1741 cggtgtggcc cgcgcgacga gctcggcgcc ggtgatgccg ctcccgccgg ggcggttgtc
     1801 cacgacgacg ggctgcttga aggcctcgct caacttctgc gcgaccagcc gcgcggtgag
     1861 atcggtggag gctccaggcg actgcgggac gatgatccgg acaggtttgg ccggatagtg
     1921 tcgtacggcg gcatcggttt gagccgctgt agtagccacc gtgtgcgcca cgccggccaa
     1981 acctgacaac aggacaactg aaatggatcg tcgcacggct cctcctctca ccggctcacc
     2041 cgtcacccgt cacccgtcac ccgtcactcg tcactcgtca ctcgtcactc aggagcgatt
     2101 ttggccgacc ggatgatatt cgcgtagagc gccgtctcct cgcgcacgat cctcgccagg
     2161 ccttccgggc cgccgccgac gacctgggcg ccgcgcttgg ccaggttttc cctgatttcc
     2221 ttgttttccc cgacgcgggc gaagtccttg tgcagcttgg cgatgacttc gggcggcgtg
     2281 ccggcgcggg cgaagatgcc catccagccg tcgagatcga actgcggaag ggtggccgca
     2341 acgcccggga catcgggcag caccggcgag cccttcgcgg cggacaccgc gatcgggcgc
     2401 accttgccgc tccagatatg cggcagcacg ccgacgatgt tggcgaacat gagctcgacg
     2461 cggccggaga ggagatcgag gaacgccggc gccgcaccct tatacggaac gtgattcatc
     2521 cggatgccgg actgggactg cagcagctcc atgccgagat gctgcaggtt gccgatcccc
     2581 gccgaggcat agttgagctg gccgggccgc gccttggcga acgcgatcag ctctttcacc
     2641 gacctcaccg gcagcgacgg atgcacgacc agcaccatcg gcgccgcgac gatctgcgtc
     2701 accggcacga ggtcgcgttg cgggttgtag gggagatcgc ggcgcaggct cggattgacc
     2761 gtgatcggtc ccgcgttgcc gagcagcagc gtgtagccgt cgggcgcgga cttcgccacc
     2821 acgtcggcgc cgacgctgcc gcccccgccg ccgcggttgt ccaccaccag c
//
LOCUS       DBNGWZ010000209         8375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_525987, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000209 DBNGWZ010000000
VERSION     DBNGWZ010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_525987"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="AC16CX_06180"
     CDS             complement(<1..363)
                     /locus_tag="AC16CX_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3012137.1"
                     /translation="MTRKHRYSTLALAVFAALSLTAGAALAQDKPAAKAPTKGKPPVS
                     DAEIRYQAGGSPLAKEEMHQDINPTAPQMTKAEFDRARQIYFERCAGCHGVLRKGATG
                     KPLTPDITLAKGTEYLKVF"
     gene            692..1693
                     /gene="cobA"
                     /locus_tag="AC16CX_06185"
     CDS             692..1693
                     /gene="cobA"
                     /locus_tag="AC16CX_06185"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR3012138.1"
                     /translation="MQRRPVRKDLRVLPGAGLRAEKLPLSSRTLGVLGANRDRAHPGM
                     VADGLALHRGQVASAGESAVRRPPRSAGAARGGKVYLVGSGPGNPELLTVRALRVLRR
                     ADVVLYDHLVSAEILALARRGAQRIYVGKERANHALPQGEINRLLVALASRGKQVVRL
                     KGGDPLIFGRGGEEIETLSSEGIPFEIVPGITAATGIAAYTGIPLTHRDHAHACVFVT
                     GHLKDGSVNLDWQALARPKQTVVIYMGLASLPIVCRELVRHGLPAETPAAIVQQGTTV
                     QQRVVTGTLANLPARAALAALRPPTLVIVGEVVKLREKLAWFEGGFAGSETALVTPT"
     gene            1899..2165
                     /locus_tag="AC16CX_06190"
     CDS             1899..2165
                     /locus_tag="AC16CX_06190"
                     /inference="COORDINATES: protein motif:HMM:NF021520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HIR3012139.1"
                     /translation="MAKPLELFQTIDAREWDPSEPMVRVLEALERLPRAHKIVMLLRH
                     EPRPLFGIFKANGYDYRCKFLPEGHFEVTVWHAADTLAASADLE"
     gene            complement(2102..3106)
                     /locus_tag="AC16CX_06195"
     CDS             complement(2102..3106)
                     /locus_tag="AC16CX_06195"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3012140.1"
                     /translation="MPEPTDPTRPERRLLARASLAGAVLVFIVVVTSAFLRLVRSGVG
                     CEDWPQCYGAPLAVAAASVAKAVPWMQLMHLLAAAGVGVAALVTVALSLQKPRRRETA
                     AAAALLGALTLFLAVLGRVAPGSQLPAVTLGNVLGGMALLALFWWLRLEGTAPARANA
                     SRGLALAAGIGLALLAAQIVLGTILSANFAARACAGVAECGVAPAEWDFAAFNPWRAP
                     AAPSASGAAGLARRTLHMAHRWSGAAVAVYFLGLAAVLWRARNVAGRGIAAAFIALPV
                     AQIGLGIVLVRFEVPLAAAVAHSGASALLTAAAASALFYSRSAEAASVSAACQTVTSK
                     "
     gene            complement(3113..4537)
                     /locus_tag="AC16CX_06200"
     CDS             complement(3113..4537)
                     /locus_tag="AC16CX_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3012141.1"
                     /translation="MKSVSRYADTALSRSLSGMGLAATRYDFAVPQDGRRALAMGWLA
                     LGLAALIGAGVFSILLVAARTPQLQHLFPVADFFRVALIVHVDLSVLVWFLALGGVLW
                     SINSTQRLVGLGWAALGLAASGTVVMSVAPFVDFGLPVMSNYVPVLDSPSFLGGLVVF
                     GAGFLLLTVRSLAAVPAVGVVIDGAAALRFGLNAAAVSAAVTLLAFAWSWATVPASLP
                     AKGYYDILFWGAGHVAQFTYTLLMLVAWLWLASVAAVSVPITPRVAVLLLGVGLAAVF
                     ITPVIYLAWGVASVEYRKLFTWLMQWGGGLAIAPFALGILWGITRGPAGDDAARPLRA
                     ALLASLALFGIGGGIGFVIHGSDVTIPAHYHGSIVGVTLALMGLVYLLLPRLGYREPH
                     PKWATWQPYLYGFGQLLHITGLLWSGGYGVQRKVAGTEQTLRSAGEIAGMGLMGLGGL
                     IAIVGGLLFAGIVLHSIWNAPRRS"
     gene            complement(4672..6303)
                     /locus_tag="AC16CX_06205"
     CDS             complement(4672..6303)
                     /locus_tag="AC16CX_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3012142.1"
                     /translation="MSGTVQRTISRPATAAGAPNGAGARAWRAIERAFDRVFEAADNP
                     WRHLGALAFFFFWIVAASGIYLYVFFDTSVEGAYRSVESLTNGQWYAGGVMRSLHRYA
                     SDAFAAAVALHLLKELVAGRFAGFRWFSWVSGVPLLWLMLASGIGGYWLVWDRLAQFV
                     AVATAEWFDVLPLFGEPLIRNFLTPAAVSDRFFTLMVFLHIGIPLLLLAGMFVHIQRV
                     NHADVVPARALGWGTFAALLALSLLRPAVSEGPADLAAAPEAIAFDWFYLAFYPLIYA
                     WSAQAVWVLSGTLTLGLLLLPFVARRTPAPPAQVDFANCNGCARCLADCPYAAIEMHA
                     RGGGRPGQKMPVVISALCASCGICTGACPSSTPFRSSPWLATGIDMPQRPINAAREAL
                     ERMLPRLRGERRIVAFGCDRGADVRWIEEAGVGALSLLCIGMLPPAFIEYALRQGADG
                     VLIAVCRDGECAYRLGGDWLKQRLTGAREPHLRTSVPRDRITVVSAGAGEEASLRGAL
                     AVFRGRLAAMPRNEPRRSSRDKLARGLAGPEKSPH"
     gene            complement(6319..6822)
                     /locus_tag="AC16CX_06210"
     CDS             complement(6319..6822)
                     /locus_tag="AC16CX_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012143.1"
                     /translation="MTQALRFLAQALLYVPFMVLIGYFSTGPAYRHLDPGEALVRLSF
                     SHAGVRVRECRQRTPEELAKLPPNMRAPLDCPRERSPVTVELEMNGELVHRVIAPPAG
                     LRRDLASTVYWRRAVPAGLHRFTARLKDKREGGFDYVKEIAVTLEPGRVLVIDFDPRQ
                     GGFIFRL"
     gene            complement(6819..>8375)
                     /locus_tag="AC16CX_06215"
     CDS             complement(6819..>8375)
                     /locus_tag="AC16CX_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3012144.1"
                     /translation="GINGYMLPWDRLAQFVVVATAEWFDVLPLFKGALIRNFIYTEAV
                     SDRLFSLLSFIHIGLPLATLALLWVHTHRVPSAKTTPPWQITATVMAALVVLSFVKPA
                     LSQEHADLAVAVTRIEYDWFYLPVYALIYRWTPGEAWLLVGGATALFFLLPWLPPKLM
                     RKGRVFHVLVHPDNRIVAAREGETVLDAALRDGIAMPFECRNGGCGVCKGTVTYGTVD
                     YGAHQPGVLTEQEKREGRALFCCATPLSDIEIEYVPVGTPGGIPVRVYVARVHKMARL
                     ADDVMQLFLKVEGDERLRYYPGQHINVILEDGAKRSFSFATAPQDSEWIELQIRRIEG
                     GRFTTRLFTEMKEGDIVRFEGPLGSFFLREDSEKPIIFVAGSTGFAPVKSMVEYAFRT
                     GMKRQMMLYWGTRSRRDLYLAELPQQWARDHPNFKFVPVLSDPRPEDNWTGRTGLVHE
                     AILQDFPDLSRYQIYACGSAAMVAAAHPAFIAHGLSQDDCFSDAFNLAPQIARATPQA
                     DMVRLGGGPA"
BASE COUNT         1515 a         2888 c         2817 g         1155 t
ORIGIN      
        1 gaacaccttc agatactcgg ttcccttggc gagcgtgatg tcgggggtga ggggcttgcc
       61 ggttgcgccc ttgcgcagca cgccgtggca gccggcgcag cgctcgaagt agatctggcg
      121 cgcgcgatcg aactcggcct tggtcatctg cggcgccgtg ggattgatgt cctggtgcat
      181 ctcctccttg gcgagcggcg agccgccggc ctggtagcgg atctcggcat cgctcaccgg
      241 cggttttccc ttggtgggcg ccttggccgc cggcttgtcc tgtgcgagcg ccgcgccggc
      301 cgtcaaagac agggcggcaa agaccgcaag ggcgagggtc gaatagcgat gtttgcgtgt
      361 cataggctgc gctcccaaaa aagaaatcat gaagaccgga aagcccagca gcctgtcgga
      421 cttagcagtc cacacaggct gctgacagca aaaccggccg acccttgagc aaaaaaccgg
      481 gcagaatggg tcgaatgtgc agaaaagcga agatgatttt gcaaattcgt catcctttct
      541 tgcaattgtc ctggagccgg ttttgcatca ggagtttgtc ggggccaagt ccgacaaact
      601 ccttcagcat cgggcaacag gatggcgcgg ggtgggttct ccgaccttga tgtagatcaa
      661 agttttccgg gggtgcgcac cctaaagtag cgtgcaacgc cgccccgtga ggaaggattt
      721 gcgggtattg ccgggggcgg gattacgagc ggagaagcta cccctgtcat cgcgcacctt
      781 gggcgtcctc ggagcgaacc gcgatcgcgc gcatccgggc atggtcgcgg acgggctcgc
      841 gctgcaccgg ggccaggtcg ccagcgcggg cgaaagcgcg gtccgtcgcc cgccgcgctc
      901 cgccggcgcc gcacgcggcg gcaaggtcta cctcgtaggc agcggtcccg gaaatccgga
      961 gctgctcacc gtgcgcgcgc tgcgcgtgct ccggcgcgcc gatgtggtcc tctacgacca
     1021 cctggtgagc gcggaaatcc tcgcgcttgc gcggcgcggc gcccagcgca tctacgtcgg
     1081 caaggagcgc gccaaccacg cccttcccca gggcgagatc aaccggctgc tggtcgcgct
     1141 cgcgagtcgg ggaaagcagg tcgtgcgcct gaagggcggt gatccgttga tcttcgggcg
     1201 cggcggagag gagatcgaaa cgctatcgag cgagggcatt ccgttcgaaa tcgtcccggg
     1261 tatcaccgcc gccaccggca tcgcggccta caccggaatt ccgctcaccc atcgcgatca
     1321 tgcgcatgcg tgcgtgttcg tcaccggaca cctcaaggac ggcagcgtca acctcgattg
     1381 gcaggcgctc gcccgcccca agcagaccgt cgtgatctac atggggctgg ccagcctgcc
     1441 catcgtctgc cgcgagttgg tgcgccacgg cctgcccgcg gaaacgcccg ccgccatcgt
     1501 gcagcagggc accaccgtgc agcagcgcgt cgtgaccggc acgctcgcca atttgccggc
     1561 gcgcgcagcg ctggcggcgt tgcggccgcc caccctcgtc atcgtgggcg aagtcgtcaa
     1621 gctgagagaa aagctcgcct ggttcgaagg cgggtttgcc gggtcagaaa ccgctctcgt
     1681 cacgccaacc taacgcgtcg cgccgttccc gctgcgccta cgcgtgcagc gccaggcagc
     1741 gcagcgcgcg ccattgaaca aaatcctgtt gcaccgcgca aaccggtccc aaccggtttg
     1801 ggcaggacgc cggacgcgga ctccggtttt cttgatgagc atcaaggttt cccgcggcgg
     1861 tcttttctag tctgggcatc ccggcaaacg gaggcgcggt ggcaaagccg ctggaactgt
     1921 tccagacgat cgatgcacga gagtgggacc cgtccgagcc gatggtgcgg gtgcttgagg
     1981 cgctcgagcg cctgccgcgc gcgcacaaga tcgtgatgtt gctgcgccac gagccgcgcc
     2041 cgctgttcgg gatcttcaag gcgaacggct acgattaccg ctgtaaattc ctgcccgagg
     2101 gtcacttcga agtgaccgtc tggcacgcgg ccgacacgct cgccgcctcc gccgatctcg
     2161 agtaaaagag cgcgctcgcc gccgcagccg tgagcagcgc cgacgcgccg ctgtgcgcga
     2221 cggccgcggc caacggcacc tcgaatcgca ccagcacgat gccgagcccg atctgcgcga
     2281 ccggcaacgc gatgaacgcc gctgcgattc cccgaccggc cacgttgcgc gcgcgccaca
     2341 gcacggcagc gaggccgagg aaatagacgg caacggccgc cccactccag cgatgagcca
     2401 tgtgcagtgt ccggcgcgcg aggccggcgg ccccggacgc cgagggggcg gcgggcgccc
     2461 gccacgggtt gaacgcggcg aaatcccact cggcgggcgc gacgccgcac tccgcgacac
     2521 cggcgcaggc gcgggccgcg aaattcgcgc tgaggatcgt ccccagcacg atctgcgcgg
     2581 cgagcaacgc caatccaatt ccggcagcga gtgccagccc cctgctcgcg ttcgcccgcg
     2641 caggcgccgt gccctcgagc cgcaaccacc agaacagcgc gagcaaagcc atgccgccca
     2701 gcacgttgcc gagcgtcacc gccggcagct gggaacccgg cgcaacgcgt ccaagcaccg
     2761 cgagaaacag ggtgagcgcg ccgagcaggg ccgcggcggc cgcggtctcg cgacgtcgcg
     2821 gcttctgcag cgataatgcc accgtcacca gcgccgccac acccacgcca gccgcggcga
     2881 gcaggtgcat cagttgcatc catggcaccg ctttggcaac ggacgcggcg gcgaccgcca
     2941 gcggtgcgcc gtagcactgc ggccaatcct cgcagcccac gcccgagcgg acaagccgca
     3001 ggaacgcgct cgtcacgacg acgatgaata cgagcaccgc gcccgcgagg gaggcgcgcg
     3061 cgagcaagcg tcgctcaggc cgggtcgggt cggtcggctc gggcatggcg cgtcacgagc
     3121 ggcgcggcgc gttccagatc gaatgaagca cgattcccgc gaacagaaga ccgccgacga
     3181 tggcgatcag gccgcccagt cccatcagcc ccatgcccgc gatctcgccc gcgctgcgca
     3241 gcgtctgctc ggttcccgcc accttgcgct ggactccgta gccgcccgac cacagcagcc
     3301 cggtgatgtg cagcagctgg ccgaagccgt acaggtaggg ctgccaggtc gcccatttcg
     3361 ggtgcggttc gcggtagccg aggcgcggca gcagcaggta gacgagcccc atcaacgcga
     3421 gcgtcacgcc gacgatggag ccgtggtagt gcgcggggat ggtgacgtcg ctgccgtgaa
     3481 tcacgaaacc gatcccgccg ccgatcccga agagcgcgag tgaggcgagc agcgcggcgc
     3541 gcaatggacg cgccgcatcg tcccccgccg gaccgcgcgt gattccccac aggatgccga
     3601 gcgcgaacgg cgcgatcgcg agccccccgc cccattgcat gagccaggtg aagagcttgc
     3661 ggtactcgac ggaggcgacg ccccaggcga gatagatgac gggcgtgatg aacaccgccg
     3721 cgagtcccac gccgagcagc agcacagcga cgcgcggggt gatcggcacg ctcaccgcgg
     3781 ccacgctcgc gagccacagc cacgccacca gcatgagcag ggtataggtg aactgcgcga
     3841 cgtggccggc gccccagaac aggatgtcgt aatagccctt ggcggggagg ctcgcgggca
     3901 ccgtggccca cgaccaagcg aaggcgagca gcgtcacggc cgccgacacc gccgccgcat
     3961 tcaggccgaa gcgcagcgcc gccgcgccgt cgatcacgac gccgaccgcg ggcaccgcgg
     4021 cgaggctgcg cacggtgagc agcaggaatc ccgcgccgaa caccaccagc cctccgagga
     4081 aggacgggct gtcgagcacc ggcacgtagt tgctcatgac cggcaggccg aagtcgacga
     4141 acggcgcgac gctcatcacg acggtgccgg aggccgcgag accgagcgcc gcccacccca
     4201 acccgaccag gcgctgcgtg ctgttgatgc tccacagcac gccgccgagc gcgaggaacc
     4261 agaccagcac cgaaaggtcc acgtgtacga tcagcgccac gcggaaaaaa tccgcgaccg
     4321 ggaacaggtg ctggagctgc ggggtgcgcg cggccaccag caggatcgag aacacgcccg
     4381 cgccgatcag cgcggcgagc ccgagcgcga gccagcccat cgcgagcgcg cgccggccgt
     4441 cctgcggcac ggcgaagtcg tagcgggtag cagcgaggcc catgcccgac agagagcggc
     4501 tgagcgccgt atcagcgtag cgcgatacgg atttcactag aagccatctc accaagcatt
     4561 agccgcagat aaacgccgat aaacgcagat gaaatttcct gcatacgatg tctctggtct
     4621 ggatgttatc agcgtccatc ggcgtctgtt taggggttct tgagagcctg tttagtgtgg
     4681 gcttttttcc ggcccggcaa gcccacgcgc gagtttatcg cgcgaagagc ggcgtggctc
     4741 gtttctcggc atggccgcaa gccttccgcg aaaaaccgcg agcgcgccgc gcagggaagc
     4801 ctcctcgccg gcgcccgccg atacgaccgt tatccgatcg cgcggcacgc tcgtgcgcaa
     4861 gtgcggctcc cgcgcgcccg tgaggcgctg cttgagccag tcgccaccca gccgatacgc
     4921 gcactccccg tcccggcaga ccgcgatcag gaccccgtcc gcgccctggc gcaacgcgta
     4981 ctcgatgaac gcgggcggca gcatgccgat gcacagcagg ctcaatgcgc cgacgcccgc
     5041 ctcctcgatc cagcgcacgt ccgcgccccg gtcgcaaccg aaggcgacga tgcgccgctc
     5101 accgcgcaat cgcgggagca tgcgctcgag cgcctcgcgc gcggcattga tcggccgctg
     5161 cggcatgtcg atgcccgtcg cgagccacgg gctgctgcga aacggggtgg aggacgggca
     5221 ggccccggtg cagatgccgc agctcgcgca tagcgcggag atgacgaccg gcattttctg
     5281 tcccggccgt ccgccgccgc gcgcgtgcat ttcgatcgcg gcgtacgggc agtccgcgag
     5341 gcagcgggcg cagccgttgc agttcgcgaa gtccacttgc gccggcggcg ccggcgtgcg
     5401 ccgcgcgacg aacggcagca gcagcaaacc cagcgtgagc gtcccggaga ggacccacac
     5461 cgcctgagcc gaccacgcgt agatcaacgg gtagaacgcg agatagaacc agtcgaaagc
     5521 gattgcctca ggcgcggcgg cgagatcggc gggaccctcg ctcacggcgg gcctgaggag
     5581 cgaaagcgcg agcaacgccg cgaacgtccc ccagccgagc gcgcgcgcag ggacgacatc
     5641 ggcgtgattg acccgctgaa tgtggacgaa cattccggcg aggagcagaa gcgggattcc
     5701 gatatggaga aaaaccatga gcgtgaaaaa acggtcgctc acggctgccg gcgtcaggaa
     5761 attccggatg agcggctcgc cgaacaacgg caggacgtcg aaccactcgg cggtcgccac
     5821 cgccacgaac tgcgcgagcc ggtcccacac cagccagtag ccgccgatgc cgctcgcgag
     5881 catgagccaa agcaacggca cgccgctcac ccaggaaaac cagcggaaac ccgcgaaccg
     5941 cccggcgacg agctccttca ggagatgaag cgcgacggct gcggcgaacg cgtccgaagc
     6001 atagcggtgg aggctgcgca tgaccccgcc cgcgtaccac tgcccgtttg tcaggctctc
     6061 caccgagcga tacgccccct cgacgctcgt atcgaagaac acgtacaggt agattccgct
     6121 cgccgcgacg atccagaaga agaaaaacgc gagcgctccg aggtggcgcc atggattgtc
     6181 cgcagcctcg aatacccggt cgaaagcgcg ctcgatcgcg cgccaggcgc gcgcgcctgc
     6241 gccgttcggt gcgcccgccg cggtagccgg cctcgatatc gtgcgctgga cagtgccgct
     6301 catggtggag cccccggctc acagccggaa gatgaatccg ccctgacgcg ggtcgaagtc
     6361 gatcaccagc acgcgcccgg gctcgagcgt gacggcgatt tccttgacgt agtcgaaacc
     6421 gccctcccgt ttgtccttca ggcgcgcggt gaagcgatgc aggcccgcgg gcactgcgcg
     6481 ccgccaatag acggtggagg cgagatcgcg ccgtaaaccc gcaggcggcg cgataacgcg
     6541 atggacgagc tcgccattca tctcgagctc gaccgtcacc ggcgagcgct cccgcggaca
     6601 gtcgagcggc gcgcgcatgt tcggcggcag tttcgcgagt tcctcgggcg tgcgctgccg
     6661 gcactccctc acgcggacgc ccgcgtggct gaaagaaagc cgcaccagcg cttcgccggg
     6721 atcgaggtgc cggtaggcgg gtccggtcga gaaatagccg atcaacacca taaacggcac
     6781 gtagagcaac gcctgggcga ggaagcgcag cgcctgggtc atgccgggcc gcctccgagc
     6841 cgcaccatgt cggcctgcgg cgtggccctc gcgatctgcg gcgcaaggtt gaacgcgtcg
     6901 gaaaagcagt catcctgcga cagcccgtgc gcgatgaatg ccgggtgcgc ggccgcgacc
     6961 atcgcagccg agccgcaggc gtagatctgg tagcgggaga gatccgggaa atcctgcagg
     7021 atcgcctcgt gcacgagccc ggtgcgcccg gtccagttgt cctcggggcg aggatcggag
     7081 agcaccggca cgaacttgaa gttgggatgg tcgcgcgccc actgttgcgg cagctcggcg
     7141 aggtacaggt cccggcgcga gcgcgtgccc caatacagca tcatctgccg cttcatgccg
     7201 gtgcggaacg cgtactccac catgctcttc accggcgcaa agccggtcga gccggcgacg
     7261 aagatgatcg gcttctcgct gtcctcgcgc agaaagaacg agccgagcgg cccctcgaag
     7321 cgcacgatgt cgccctcctt catctcggtg aaaagtcggg tggtgaagcg gccgccttcg
     7381 atccggcgga tctgcagctc gatccattcc gagtcctgcg gcgccgtcgc gaacgaaaag
     7441 ctccgtttcg cgccgtcttc gaggatgacg ttgatatgct ggcccgggta gtagcgcagc
     7501 ctctcgtcgc cctcgacctt gaggaacagc tgcatcacgt cgtcggcgag ccgcgccatc
     7561 ttgtgcacgc gcgccacgta gaccctgacc gggatcccgc ccggcgtgcc gaccggcacg
     7621 tattcgatct cgatgtcgga gaggggtgtg gcgcagcaga acagcgccct gccctcgcgc
     7681 ttttcctgct cggtgagcac gcccggctgg tgcgcgccgt agtcgactgt tccgtaggtc
     7741 accgtgccct tgcacacgcc gcagccgccg ttgcggcact cgaacggcat ggcgatcccg
     7801 tcgcgcagcg ccgcgtcgag cacggtttcg ccctcgcggg cggcgacgat gcggttgtcg
     7861 ggatgcacca ggacgtggaa caccctgccc ttgcgcatga gcttgggcgg cagccaaggc
     7921 agcaggaaaa acagcgcggt tgcgcccccc accagcagcc acgcctcgcc cggcgtccag
     7981 cgatagatga gcgcgtacac cggcaggtag aaccagtcgt attcgatgcg ggtcaccgcc
     8041 accgcgagat ccgcgtgctc ctggctgagc gcaggcttca cgaaggacag cacgacgagc
     8101 gccgccatca ccgtcgcggt gatctgccac ggcggcgtgg tcttcgcgct cggcacgcga
     8161 tgggtgtgca cccacagcag cgcgagcgtc gccagcggca gcccgatgtg gatgaaggag
     8221 agcagcgaga acagccggtc gctgaccgcc tcggtgtaga tgaagttgcg gatcagcgcg
     8281 cccttgaaca gcggcaggac gtcgaaccac tcggcggtcg cgaccaccac gaactgcgcc
     8341 aggcggtccc acggcagcat gtagccgttg atgcc
//
LOCUS       DBNGWZ010000210         8054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2918966, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000210 DBNGWZ010000000
VERSION     DBNGWZ010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2918966"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="AC16CX_06220"
     CDS             <1..838
                     /locus_tag="AC16CX_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3012145.1"
                     /translation="LVLALALAGAYLIVARINRPLRELTRAADEIGRGKTPPPVVETG
                     PSEIRTLARAFNQMTADLKRLDDERALLLAGVSHDLRTPLSRIRLALEMMDDKGDAAL
                     KAGLVQDIEDIDAAINQFLDFARIGEAEPAVTDGDLNAIVREVCERSTRSGKTVTARL
                     ETLPPLRLRPTAMQRLVANLVENALRHGRGEAEVTTAVQNGTAVLEVLDRGPGIPPGQ
                     AERMLQPFTRLDAARSSPGTGLGLAIVERIARMHGGSVQLLAREGGGLRARVELPLKT
                     PN"
     gene            complement(958..1542)
                     /locus_tag="AC16CX_06225"
     CDS             complement(958..1542)
                     /locus_tag="AC16CX_06225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3012146.1"
                     /translation="MIVRVNQLKSGALAIDSLGNQVMIRQCQPAELDDICAVINDGAA
                     AYRGVIAADCWNDPYMSGGELRRELDAAVLFSGCYEGGRLLAVMGLQQVGDVALIRHA
                     YTRTANQGTGMGTALLAHLRSQTDRPMLVGTWKAATWAVRFYQSRGFGLVNAPQKDAL
                     LRRYWTVPERQIEESVVLADAQWFARSSTTPDAA"
     gene            complement(1672..2574)
                     /locus_tag="AC16CX_06230"
     CDS             complement(1672..2574)
                     /locus_tag="AC16CX_06230"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3012147.1"
                     /translation="MQWKFELLMKPSAVPLTEGPAWDGEHVYFTHIRASRILRYDLKS
                     GTITEWRSGTNRTNGLAFDSQGRLFGCCSGGRSILRFDPDGRNVTIAGRVDGKRLNTP
                     NDLAIDRKGRIWFTNPVNEGNWDKTEVKELDNQSVLRADPQPGGSYTVTRVTFDTTMP
                     NGILVSQDQGTLYVAESGYARGIARELRAYPIREDGSLGAYATLFTWGEDARGVHRGI
                     DGMCLDRDGNIVATAGWEVSGSGPMIYVFSPSGRVLETQPVPANRPTNCCFGGADMAT
                     LFVTTVEGHFLKAQTDRVGWAMYP"
     gene            complement(2635..3117)
                     /locus_tag="AC16CX_06235"
     CDS             complement(2635..3117)
                     /locus_tag="AC16CX_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3012148.1"
                     /translation="MNRADHIAVFRQSASGAVPASDRLLLLDWSDADWNKLLDCTRPR
                     PFHASEVVIQRGTTDRALQFVVAGSLEVGITQVDGVSISPLAKITAGSVIGEQSFFDG
                     QPRSANVWGVSDGELLRLEYDGFVKFSQEEVALARDLLFALARILSSRLRNTTFRVRR
                     "
     gene            complement(3292..3831)
                     /gene="thpR"
                     /locus_tag="AC16CX_06240"
     CDS             complement(3292..3831)
                     /gene="thpR"
                     /locus_tag="AC16CX_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR3012149.1"
                     /translation="MAAETTDTARLFFALWPDTGVQRALGALAQDLSRECGGRAPAAR
                     NIHLTLVFLGNVERERLPRLEEIAVATSAPPFDLNVDRVEYWRHNRIIWAGVEQCPEA
                     LAGLVARLERALAPEGFRFDDRAYVPHITLLRNARRAPLSAAVPVVAWPVSRYALVES
                     VPRGQGRLYEVLREWPLVG"
     gene            complement(4362..4841)
                     /gene="ispF"
                     /locus_tag="AC16CX_06245"
     CDS             complement(4362..4841)
                     /gene="ispF"
                     /locus_tag="AC16CX_06245"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3012150.1"
                     /translation="MRVGQGFDAHALVAGRRLVIGGVEIPHEKGLAGHSDADVLIHAV
                     CDALLGAAGLGDIGTHFPDSDARFKDIDSRKLLREVAQLLRRHKLRVVNVDATVIAQA
                     PRMAPHLPAMRAHLASDLGVAEDAVNVKAKTTERLGFVGRGEGIAAEAIALLGDAGK"
     gene            complement(4873..5631)
                     /gene="ispD"
                     /locus_tag="AC16CX_06250"
     CDS             complement(4873..5631)
                     /gene="ispD"
                     /locus_tag="AC16CX_06250"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3012151.1"
                     /translation="MSKVYALIPAAGSGSRVGAALPKQYLEIAGRPLLYYALRALARH
                     PRIEQVFVVLAQGDDRFARCDWREFGGSIKPLYCGGETRAASVFNGLLATRDTIAAND
                     WVLVHDAARPCLGREELDRLFGELEEDDTGGLLAMPVADTLKRASRESRVAATEPRDN
                     LWLAQTPQMFRYRLLIEALRAADPAVVTDEARAIEGLGLKPRLVMGDARNIKVTFTED
                     LVLAELILGSHQPSAVSRQRKTRRAAPRKRKKRG"
     gene            5732..6556
                     /gene="amrB"
                     /locus_tag="AC16CX_06255"
     CDS             5732..6556
                     /gene="amrB"
                     /locus_tag="AC16CX_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR3012152.1"
                     /translation="MLQSTPSVRSPAVAGMFYPGESHELSHSLAQMLGAAAHDAPERA
                     VPKAIIAPHAGYIYSGPIAASVYTLLAPARRRITRVVLLGPTHRVAVRGLALPGCSAF
                     ATPLGTVEIDQAAVEALHGLPQVMVSPQAHALEHSLEVHVPFLQTVFERFTLVPLAVG
                     HASAPEVAEVLEASWGGPETLIVVSSDLSHYLSYGEAQAVDRATAKAILGLATDISHE
                     QACGATPVTGLTHIARQRGLKPELIDLRNSGDTAGDKNRVVGYGAFAFYEDTRDAR"
     gene            6546..7196
                     /gene="amrA"
                     /locus_tag="AC16CX_06260"
     CDS             6546..7196
                     /gene="amrA"
                     /locus_tag="AC16CX_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HIR3012153.1"
                     /translation="MPANSGPIVLSSGSGHIVLQSGGPLPYPSDAGHVLLPLARAAIA
                     AELGLSLQKMNEDPPWLRQQGACFITLTRDDRLRGCIGTLRAHRALADDVKANAVAAA
                     FRDPRFAPLTAVEFAAVALEISLLSMLQPMSFADEPDALRQLRAGVDGVVFEYGHHTS
                     TFLPQVWEDLKEPTEFLAHLKYKAGLPPDFWDKEVKLSRYTVFKWCESDKGVTSDR"
     gene            7280..>8054
                     /gene="amrS"
                     /locus_tag="AC16CX_06265"
     CDS             7280..>8054
                     /gene="amrS"
                     /locus_tag="AC16CX_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR3012154.1"
                     /translation="MSATREHPGRWWHMLEDGRLQCDLCPRDCRLHEGQRGACFVRMR
                     EHDRMVLTTYGRSSGFCIDPVEKKPLNHFYPGTSILSFGTAGCNLACKFCQNWDISKS
                     RDMDRLADEASPEAIAEAAAAYGCKSVAFTYNDPVIFAEYAMDIADACHERGIQAVAV
                     TAGYIHAEPRSEFYAKMDAANVDLKGFTEDFYFKLCAAKLQPVLDTLVYLKHETRVWF
                     EITTLLIPGKNDSSAEIEAESKWIMKELGPDVPVHFTAFH"
BASE COUNT         1390 a         2727 c         2523 g         1414 t
ORIGIN      
        1 gctggtgctg gcgctcgcgc tcgccggagc ctatctcatc gttgcgcgca tcaaccgccc
       61 gctgcgcgag ctcacccgcg cggcggatga aatcggccgc ggcaagacgc cgccgcccgt
      121 cgtcgaaacc gggccctcgg agatccgcac cctcgcccgc gccttcaacc agatgacggc
      181 ggacctcaag cggctcgacg acgagcgcgc gctgctgctg gcgggcgtgt cgcacgatct
      241 gcgcactccg ctctcgcgca tccgcctggc tttggaaatg atggacgaca agggtgacgc
      301 ggcgctgaag gccggactgg tgcaggacat cgaggacatc gacgccgcga tcaaccagtt
      361 cctcgacttc gcgcgcatcg gggaagcgga gcctgcagtg accgacggcg acctcaacgc
      421 catcgtgcgc gaggtttgcg agcgcagcac ccgctcgggc aaaacggtca cggcgcggct
      481 tgaaacgctg ccgccgctgc ggctgcgccc gaccgccatg cagcggctgg tcgccaacct
      541 ggtggaaaac gcgctgcgcc acggcagggg cgaagcggaa gtcacgaccg ccgtgcagaa
      601 cggtacggcg gtgctggaag tgctggatcg cgggcccggc attccgcccg ggcaggccga
      661 gcgcatgctg cagccgttca cgcgcctcga tgcagcccgc tcgagccctg gcaccggcct
      721 gggactcgcg atcgtcgagc gcatcgcccg catgcacggg ggcagcgtgc agctgctcgc
      781 gcgcgagggc ggcggtctgc gcgctcgcgt ggaactaccg cttaaaaccc cgaattagcc
      841 gccaagagcg ccaagaagat caaaaagaga atacgagaaa acaatcggac gcgttaacgc
      901 ctcggcatca gccgtggcgc gacccgaaat cacgagcgcc gtcggctgca tcgcgtgtta
      961 cgcggcgtcc ggtgtcgtcg aagacctcgc gaaccactgc gcgtcggcca gtacgaccga
     1021 ttcttcaatc tgccgctccg gaaccgtcca gtagcgtcgc agcaacgcgt ccttctgggg
     1081 tgcgttgacg agcccgaacc cgcggctctg atagaaccgg acggcccaag tcgcggcttt
     1141 ccaagtcccc acgagcatgg ggcgatcggt ctgactccga aggtgggcga gaagcgccgt
     1201 gcccattcct gtcccctgat tggcggtacg agtgtaggca tgccggatca gcgcgacatc
     1261 gcctacctgt tgcagcccca tgacggcaag gagccgcccg ccttcgtaac atccagaaaa
     1321 cagcaccgcc gcatccagct cgcgacgcag ttcgcccccg gacatgtacg ggtcgttcca
     1381 gcaatccgca gcgatgacgc cacgatacgc tgcggccccg tcgttgatca ctgcacagat
     1441 gtcatcgagc tcggccggct gacattgacg gatcatcact tgattgccaa gagaatctat
     1501 cgccagcgcg cctgatttca attggttgac acgcacgatc acgtcgttgg atcttactcg
     1561 ttcttgtttt ttattttggt cttcgttttt cctggcgcgc ttggcgtctc ggcggcgtgt
     1621 tatggttctc ttggcggcct tggcgcctta gcgtctattt tctgcttgcc gctacgggta
     1681 catcgcccag cctacccggt cggtctgggc cttgaggaaa tgtccttcca cggtggtaac
     1741 gaacagggtc gccatgtcgg cgccgccgaa gcagcaattg gtcggacggt tcgccggcac
     1801 cggctgggtc tcaagcaccc ggcctgaagg cgagaacacg tagatcatcg gccccgatcc
     1861 gctcacttcc cagccggcgg tggcgacgat gttgccgtcg cggtcaaggc acatgccatc
     1921 aatgccgcga tgtacgcccc tggcgtcctc cccccaggta aacagcgtcg cataggcgcc
     1981 gaggctgccg tcctcccgga tcggataggc gcgcagctcg cgcgcgatcc cccgtgcgta
     2041 tccgctttcg gcgacgtaca gcgtaccctg gtcctgcgat accaggatgc cgttgggcat
     2101 cgtggtatcg aacgtgacgc gcgtgaccgt gtagctgccg cccggctgcg gatcggcgcg
     2161 cagcaccgac tggttgtcga gttccttcac ctcggtcttg tcccagttgc cctcgttgac
     2221 cgggttggtg aaccagatgc gccccttgcg atcgatcgcg aggtcgttcg gcgtattgag
     2281 cctcttgcca tccacgcggc cggcaatggt gacgttgcgg ccatcgggat cgaagcgcag
     2341 tatcgagcgc ccgcccgagc agcagccgaa gaggcgcccc tgtgaatcga acgccagccc
     2401 gttggtgcgg ttggtcccgc tgcgccattc cgtgatcgtg ccgcttttca ggtcgtagcg
     2461 caggatgcgg ctcgcccgga tgtgcgtgaa gtacacgtgc tcgccgtccc acgcggggcc
     2521 ctcggtcagc gggacagccg acggcttcat caacagttcg aatttccact gcattcttgg
     2581 cggccttggc ggttcgataa ttgattttct tggggtgctt ggcggttcag ctttttaccg
     2641 gcgcacccgg aaggtggtgt tgcgcagccg cgaggacagt atccgcgcca gcgcgaacag
     2701 caggtcccgc gccagcgcca cttcctcctg gctgaacttc acgaagccgt cgtattccag
     2761 ccgcagcagc tcgccgtcgg agacgcccca cacattggcc gagcgcggct ggccgtcgaa
     2821 gaacgactgc tcgccgatca cgctgccggc ggtgatcttg gcgagcggcg agatgctgac
     2881 tccgtccacc tgggtgatgc cgacctcgag cgagccggcg acgacgaatt ggagcgcgcg
     2941 gtcggtcgtg ccgcgttgta tgaccacctc gctcgcgtgg aacggccgcg gtcgggtgca
     3001 gtcgagcagc ttgttccagt ccgcgtcgct ccaatccagc aggagcagcc ggtcactggc
     3061 cggcaccgct ccgcttgctg attgcctgaa caccgcgatg tgatcggcgc gattcacgcc
     3121 cgctcccctg tcataccgtt gctgttggtc gctcgatgcg gctggcggca ttcgctgaat
     3181 gaagacgcgc tgaacggcga ttgattcgtc cgttcgaatg cgcagagtta aaccggagcg
     3241 aagcttcccc ggtttaacga taattatacg cgctacaacc catccagatc gttatcccac
     3301 aagaggccac tctcgcaaaa cctcgtaaag acgcccctgc ccgcgcggca ccgactccac
     3361 cagcgcatac cggctgaccg gccaggcgac gacgggcacg gcggccgata atggcgcgcg
     3421 gcgcgcattg cgcagcagcg taatgtgggg cacgtaggca cgatcgtcga agcggaagcc
     3481 ttccggtgcg agcgcccgct cgagacgcgc caccaaccct gccagcgcct ccgggcactg
     3541 ctccactcca gcccagatga tgcggttgtg gcgccagtac tcgacgcgat ccacgttgag
     3601 atcgaagggc ggggccgaag tcgcgacggc aatctcctcg agccgcggta atcgctcgcg
     3661 ctcgacgttg ccgagaaaca ccagcgtgag atggatgttg cgcgcggccg gggcgcggcc
     3721 gccgcactcg cgactcaggt cctgcgccag cgcacccagg gcgcgctgaa caccggtgtc
     3781 gggccacagg gcgaaaaaca gccgcgcggt gtcagtggtt tcggccgcca ttggctccgc
     3841 tcagatgcgt gaatggtgag tagtgagtgg taagaagact ttcatgttgc atgaatactt
     3901 ccatccctgt taaccgcgtg ttgaacggtg acaattcatc cgttccaaca cgcgcggcct
     3961 ccgaacggtc atcacgaaag ccgaagtgta cgcgtcggcc gccatgcgcg cagcctccgc
     4021 accgcttgag tcaagaatcc caatttacga tgtcggctgc attgcgcgtc tacgcggtcg
     4081 catccccgcg agcgggggcc ctgctcaacg ctccgacgcg ccctccttgg gtcgcacccg
     4141 caagggtagc gcgtcgcagg gcggaagagg ggccctggtg attttccggg gatctgaccc
     4201 cggagacgcg cgtgggatcg gctttctcgc gacgcgccga cgatcggctc cacggtcgca
     4261 tcccataaac gggcccctgc tccacgttcc gccgatccct gctcaacctt ccggcgcgct
     4321 caaaccggcg catagcgtcc ggatttaacc gcacttggcg gctacttgcc ggcatccccc
     4381 aagagagcga ttgcttctgc cgcgataccc tcgccccgcc cgacgaagcc aagccgttcg
     4441 gttgtcttcg ccttgacgtt gactgcatcc tcggcgacgc caaggtcgga agcaagatga
     4501 gcacgcatcg cgggaagatg cggcgccatt ctgggcgcct gcgcgatgac ggttgcatcc
     4561 acgttcacaa cgcgcagttt gtgccgccgc agcagctgcg ccacctcgcg cagcagtttg
     4621 cggctgtcga tgtccttgaa gcgcgcgtcg ctgtcgggaa aatgcgtgcc gatatcgccc
     4681 aatcctgccg cgccgagcag cgcgtcgcac accgcgtgga tcagcacatc agcgtcggag
     4741 tgtccggcca gccctttttc gtgcggaatc tccaccccgc cgatgacgag cctgcgtcca
     4801 gccaccagcg cgtgggcgtc aaacccctgc cccaccctca tgattcagct ctcacctatc
     4861 agcattcagc gatcagcctc gtttcttcct tttccgcggt gccgcgcggc gggtcttccg
     4921 ctgacggctg accgctgacg gctgatggct cccgaggatc agctctgcca agaccaggtc
     4981 ctcggtaaat gtcaccttga tgttgcgcgc atcgcccatg acgagcctgg gcttcaggcc
     5041 caggccctcg atggcgcgcg cttcatcggt cacgaccgcg ggatcggcgg cgcgcaacgc
     5101 ctcgatcagc agacggtaac ggaacatctg cggcgtctgc gcgagccaca gattgtcgcg
     5161 cggttcggtc gccgccacgc gcgattcgcg gctggcgcgc ttcaacgtgt ccgccaccgg
     5221 catcgcgagc aacccgccgg tatcgtcctc ctcgagctcc ccgaacaggc gatccagctc
     5281 ctcgcgcccg aggcatggcc gcgcggcatc gtgcaccaac acccaatcgt tagcggcgat
     5341 agtgtcgcgc gtggcgagca ggccgttgaa cacgcttgcc gcgcgcgtct cgccaccgca
     5401 gtaaagcggc ttgatgctcc cgccgaattc gcgccagtcg cagcgcgcaa aacggtcatc
     5461 gccttgcgcg agcaccacga atacctgctc gatgcgcggg tgccgcgcga gcgcccgcaa
     5521 cgcgtaatag agcagcgggc gccccgcgat ctccaggtat tgcttgggca gggccgcgcc
     5581 cacgcgggaa ccggagccag cggcgggaat gagcgcgtag accttcgaca cgtgtgggga
     5641 attcactgta attgcaggga ttttatcagc aacaggagtt gtatcctgcg cacttgaaaa
     5701 accgcctgcc gggcgcataa ttgaaaggca aatgctgcaa tccaccccgt ccgtacgctc
     5761 gccggccgtc gccggcatgt tctacccagg cgaaagccac gaactttcgc atagcctcgc
     5821 gcagatgctg ggcgcggccg cgcacgacgc ccccgagcgc gccgtgccca aggcgatcat
     5881 cgcgccgcac gcgggttaca tctattccgg gccgatcgcg gccagcgtct acaccctgct
     5941 cgccccggcg cgccgccgca tcacgcgtgt cgtgctgctc gggcccaccc accgcgtcgc
     6001 ggtgcgtgga ctggcccttc ccggctgcag cgcttttgcc acgccgctcg gcacggtcga
     6061 gatcgaccaa gccgcggtcg aggcgctgca cggcctgccg caggtgatgg tgagcccgca
     6121 ggcgcatgcc ctggagcatt cgctggaagt gcatgtgccg ttcctgcaaa cggtgttcga
     6181 gcgcttcacg ctggtgccgc ttgcggtcgg tcacgccagc gcgccggagg tcgcggaagt
     6241 cctcgaagcg tcgtggggcg gtcccgagac gctgatcgtg gtgagttccg acctctcaca
     6301 ttatctttcc tacggcgaag cccaggccgt ggaccgcgcg accgccaagg ccattctcgg
     6361 gctcgcgacc gacatctctc acgagcaggc gtgcggcgcg acgccggtga ccgggctcac
     6421 ccacattgcg cgccagcgcg ggctgaagcc cgagctgatc gacctccgca attcgggcga
     6481 caccgccggg gacaagaacc gcgtcgtcgg ctacggcgct tttgctttct acgaggacac
     6541 acgggatgcc cgctaattcc ggccccatcg tcctgtccag cgggtcgggc catatcgttc
     6601 tgcaaagcgg cggcccgctt ccgtacccct ccgacgccgg acacgtgctg ctgccgctcg
     6661 cgcgcgcggc gatcgcagcc gagctcgggc tgagcctcca gaagatgaat gaagacccgc
     6721 cgtggctcag gcagcaaggc gcctgtttca tcaccctgac gcgtgatgac aggctgcggg
     6781 ggtgcatcgg cacgctgcgc gcgcaccgcg cgctcgccga tgacgtcaag gccaacgcgg
     6841 tggcggcggc tttccgcgat ccgcgctttg cgcctctcac cgcggtggaa ttcgccgcgg
     6901 tcgcgctcga aatctcgctg ctttccatgc tccagcccat gagcttcgcc gacgagccgg
     6961 acgcgctcag gcagttgcgt gccggggtgg acggcgtggt gttcgagtac ggccaccata
     7021 cgagcacgtt cctgccccag gtgtgggagg acctgaagga accaacggag ttcctggccc
     7081 atctcaagta caaggcgggg ctgccgcctg atttctggga caaggaggtc aagctctcgc
     7141 gctacaccgt cttcaagtgg tgcgagtctg ataaaggcgt gacaagtgac aggtgataag
     7201 tgataagggc ttaacgacaa accgatcttc cggcccctat cacctatcac atatcactta
     7261 tcacccagtg caaagcacga tgagcgcaac gcgcgaacat ccgggccggt ggtggcacat
     7321 gctggaagac ggcaggctcc agtgcgacct gtgcccgcgc gattgccgcc tgcacgaggg
     7381 ccagcgcggg gcctgtttcg tgcgcatgcg cgagcacgac cgcatggtgc tcaccaccta
     7441 cggccgctcg tccggcttct gcatcgatcc cgtcgagaaa aagccgctca accacttcta
     7501 tccgggcacg agcatcctgt ctttcggcac ggcgggctgc aacctcgcct gcaagttctg
     7561 ccagaactgg gacatttcca agtcgcgcga catggaccgg ctcgccgatg aggcctcgcc
     7621 ggaggcgatc gcggaggcgg cggcggccta cggttgcaag agcgtcgcct tcacctataa
     7681 cgatccggtg atcttcgccg agtacgcgat ggacatcgcg gacgcctgcc acgaacgcgg
     7741 catccaggcc gttgcagtca ccgcgggcta catccatgcc gagccgcgca gcgagtttta
     7801 cgcgaagatg gacgccgcaa acgtagattt gaagggtttc actgaagatt tttatttcaa
     7861 gctgtgcgcg gcgaagctgc agccggtcct ggacaccctc gtttacctca agcacgaaac
     7921 ccgcgtctgg ttcgagatca cgacgctgct catccccggc aagaacgatt ccagcgcgga
     7981 aatcgaggcc gagagcaagt ggatcatgaa ggagctgggg cccgacgtgc cggtgcactt
     8041 caccgcgttt cacc
//
LOCUS       DBNGWZ010000211         3420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_650856, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000211 DBNGWZ010000000
VERSION     DBNGWZ010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_650856"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC16CX_06270"
     CDS             <1..558
                     /locus_tag="AC16CX_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3012155.1"
                     /translation="NLGIAGMAESGVQGRGVMIDLRHHFGDERVEVDYEMLVKVMKAD
                     GVQVERGDIVCIHTGLGQLIRDADGKLDPSIRHACAVMNGYDKKLLEWIADTGLAAIA
                     CDNLAVERSSTLGADPRLPHRGPALPLHEHCLVKLGIHLGELWYLTELAAWLRENRRG
                     RFLLTAPPLRLPGAAGSPVTPVATV"
     gene            735..1361
                     /locus_tag="AC16CX_06275"
     CDS             735..1361
                     /locus_tag="AC16CX_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR3012156.1"
                     /translation="MTDMTVRIHPLAVRITHWINAFAILIMIVSGWRIYNASPLFDFK
                     FPNDWTLGGWLAGALQWHFAAMWLLALNGLAYLAYGIMSGHFRSKLLPISPAAVFRDI
                     AAALRGKLAHDDLRIYNAAQRAAYLGAILLGVALVLSGLVLWKPVQFYTLGLLMGDYE
                     GARYVHFFAMAALVLFVIVHIVMVALVPRTFPAMFTGRARVPAATRGE"
     gene            1381..2124
                     /locus_tag="AC16CX_06280"
     CDS             1381..2124
                     /locus_tag="AC16CX_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3012157.1"
                     /translation="MRQSLKDQPRPQIEKIERRLFLRQGLSLGAVAMLSGCSVTDAES
                     VQKALWAMSRWNDRVQGWIFDPNRLAPEFPESAITMPFPFNAFYGMDEVPRIDASSYQ
                     LEVAGLVREKRPWTLPELYALPQVSQVTRHICVEGWSAVGKWSGVRFGDFLRRIGADL
                     TAKYVGFRCADKYYTSIDMPTALHPQTLLTFRFADQILPAEFGFPMKLRMPTKLGFKN
                     PKHIAAMFVTNEYPGGYWEDQGYNWFSGS"
     gene            2188..2571
                     /locus_tag="AC16CX_06285"
     CDS             2188..2571
                     /locus_tag="AC16CX_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012158.1"
                     /translation="MKKLVAVAVACCLSLAAGGAFAQGMMKDEMTKGEAKGEMKKGEM
                     KKDMMGKGEMKEGEMKKKAMGKDDMKDMAKGGMMKEGEMKKDKMKKDKKKKDAMGKGD
                     MKKDMAKGDMMKKGEMKKDMMDEKK"
     gene            complement(2644..>3420)
                     /locus_tag="AC16CX_06290"
     CDS             complement(2644..>3420)
                     /locus_tag="AC16CX_06290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3012159.1"
                     /translation="AMRVAGPDIWGEPKDRAAMRRLLQRAFELGHTFFDTADSYGPAV
                     SEILIAEALHPYPEELVIGTKGGLVRPNRHSWDEDGRPEHLRQAVDGSLKRLRLERID
                     LYQLHAPDPRVPYTESVGTLAELQRAGKIRHLGVSNVSVRQLDEARRIAPIVSVQNEY
                     NIENRESDAVLAACGKAGLAFIPWYPLGAGRALRSAQVKRVAARLGATPAQVALAWLL
                     ARSPVMLPIPGTGSIAHLEENAAAAGLRLSAEDLAALDKA"
BASE COUNT          649 a         1046 c         1153 g          572 t
ORIGIN      
        1 aacctgggca ttgccggcat ggccgaatcc ggcgtacagg ggcgcggggt gatgatcgac
       61 ctgcgccacc atttcgggga cgaacgcgtc gaagtggact acgagatgct ggtgaaggtc
      121 atgaaagccg acggcgttca ggtggagagg ggcgacatcg tctgcatcca tacgggcctc
      181 ggccagctga tccgggacgc cgacggaaag ctcgacccgt ccatccggca cgcctgcgcg
      241 gtgatgaacg gttacgacaa gaagctgctg gaatggatcg ccgataccgg tctggcggcg
      301 atcgcctgcg acaacctcgc ggtggaacgg tcctccacgc tcggcgcgga cccgcgcctg
      361 ccgcaccgcg gtccggcgct tcccctgcac gagcattgcc tggtcaagct cgggatccat
      421 ctcggcgagc tgtggtacct gacggaactc gcggcgtggc tgcgcgaaaa ccgccggggt
      481 cgcttcctgc tgaccgcgcc gccgttgcgg ctgcccggcg ccgcaggctc tccggtcacg
      541 cccgtcgcca ctgtctagga gtgacccggg cccgtacgcg gtttcagcga gcaaggccgc
      601 caggcccggg gtggcggtta tccgcaaggg cgccaggaag ccaccgcatc caaaatgctc
      661 taccctgcgt aatcacccag ggaccgccgc gccgcaggag aggcgaggcg ggcggtaaaa
      721 gcgggagcag gcacatgacg gacatgaccg ttcggattca tccgttggcc gtacgaatca
      781 cgcattggat caatgccttc gcgatcctca tcatgatcgt atcgggttgg cgcatctata
      841 acgcctcgcc gctcttcgac ttcaagtttc ccaatgactg gacgctgggg ggctggctgg
      901 ccggggcact gcagtggcat ttcgcggcga tgtggctgct ggcgttgaac gggctcgcgt
      961 acctggcgta cggcatcatg tccggccact tccggagcaa gctgctgccg atctcgcccg
     1021 cagccgtgtt ccgcgacatc gccgccgccc tgcgtggcaa gctcgcgcac gacgatctga
     1081 ggatttacaa cgccgcgcaa cgcgccgcct atctgggcgc catcctgctc ggcgtcgccc
     1141 tggtgctttc agggctcgtg ctctggaagc ccgtgcagtt ctacacgctc ggccttctca
     1201 tgggcgatta cgagggcgct cgttacgtgc atttcttcgc gatggcggcg ctggtgctgt
     1261 tcgtgatcgt ccacatcgtc atggtggcac tcgttccgcg caccttcccg gccatgttca
     1321 ccggtcgcgc ccgggttccg gccgcgacga gaggggaatg acatgaaacg cctgttcggg
     1381 atgcggcaga gcctcaagga ccagccccgg ccgcagatcg agaaaatcga gcggcggctg
     1441 ttcctgagac agggcctaag tctgggcgcg gtggcgatgc tgtccggctg cagcgtgacg
     1501 gatgcggaat cggtgcagaa agcgctgtgg gcgatgtccc gctggaacga ccgcgtgcag
     1561 ggctggatct tcgatcccaa ccggctcgcg cccgagtttc cggaaagcgc gatcacgatg
     1621 ccattcccgt tcaacgcctt ctacgggatg gacgaagttc cccggattga cgcctccagc
     1681 taccagctcg aggtcgccgg cctggtgcgc gagaagcggc cgtggacgct gccggagctc
     1741 tacgccctgc cgcaggtgtc gcaggtcacc cgccacatct gcgtcgaagg ctggagcgcc
     1801 gtcggcaagt ggagcggcgt gcgcttcggc gatttcctcc ggcgcatcgg ggcggacctc
     1861 accgcaaagt acgtcggatt cagatgcgcg gacaagtatt acacgagcat cgacatgccg
     1921 acggcgctgc acccgcaaac gttgcttacg ttccggtttg ccgatcagat tctgcctgcg
     1981 gagttcggat ttcccatgaa gctgcgcatg ccgaccaagc ttggattcaa aaaccccaag
     2041 cacatcgccg cgatgttcgt gaccaacgag tatccaggcg gttactggga ggaccaggga
     2101 tacaactggt tcagcggatc gtgaggtttt gcagtacaca ataagccagc agcgatgccg
     2161 atctacaacc tagactagga gatgagcatg aaaaagctgg tcgcagtcgc agttgcgtgt
     2221 tgcctgtcgc tcgccgcggg cggcgctttt gcccaaggca tgatgaagga cgagatgaca
     2281 aagggcgagg cgaaggggga gatgaaaaag ggtgaaatga agaaggacat gatgggcaaa
     2341 ggcgaaatga aggaaggcga gatgaagaaa aaagccatgg gcaaggatga catgaaggac
     2401 atggccaagg gcggcatgat gaaggaaggc gagatgaaga aagacaagat gaagaaagac
     2461 aagaagaaaa aagacgccat gggcaagggc gacatgaaga aagacatggc caagggggat
     2521 atgatgaaga agggcgagat gaaaaaagac atgatggacg agaaaaagta gccagggcgc
     2581 cgcgacgccg cgccggggcc ggccatcgcc ggcccttgct tttttcagtg gccgccgcac
     2641 ccgtcacgct ttatccaggg cggcaagatc ttccgctgac aacctgagcc ccgccgccgc
     2701 ggcgttttcc tccagatggg cgatcgagcc tgtcccgggg atcggcagca tcacgggcga
     2761 gcgcgcgagc agccacgcga gcgcgacctg cgcgggcgtt gcgccgaggc gcgcggcgac
     2821 gcgcttgact tgcgccgagc gcagcgcgcg ccccgcgccg agggggtacc acgggatgaa
     2881 agcgaggccg gctttcccgc acgcggcaag caccgcatct gactcgcggt tctcgatgtt
     2941 gtattcgttc tgcaccgaga cgatgggggc gatgcggcgc gcctcgtcga gctgccgcac
     3001 ggacacgttg gagacgccga ggtggcggat cttgcccgcg cgctgcagct ctgcgagcgt
     3061 gcccaccgac tcggtgtacg gcaccctcgg atccggcgcg tgcaactggt agaggtcgat
     3121 gcgttcgagc cgcaggcgct tcaggctgcc atccaccgcc tggcgcagat gctcgggccg
     3181 cccgtcctcg tcccagctgt gccggttcgg gcgcacgagc ccgcccttgg tcccgatcac
     3241 cagctcttcg ggataaggat ggagtgcttc ggctatgagg atctcggaaa cagcggggcc
     3301 gtagctgtct gccgtatcga agaacgtgtg tccgagctcg aaggcgcgct ggagcagcct
     3361 gcgcatcgcg gcgcggtcct tgggctcgcc ccagatgtcc gggcccgcga cgcgcatcgc
//
LOCUS       DBNGWZ010000212         4745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2289180, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000212 DBNGWZ010000000
VERSION     DBNGWZ010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2289180"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1402)
                     /locus_tag="AC16CX_06295"
     CDS             complement(26..1402)
                     /locus_tag="AC16CX_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3012160.1"
                     /translation="MSEPAQAPRQGAGGQPRQTLSVFDAVAMIVGIVIGVGIFKAPSI
                     VAGNVSGETAFIGVWLAGGVISLVGALCYAELGSSHPNAGGEYYFLSKAYGDWLGFLF
                     AWARMTVIQTGAIAAIAYVVGDYTTTLFSLGEYSSALYAGLAVIAITALNATGTSESK
                     WVQNALTVALAAAILAIIVGGVMVSPAPAAAPDPAKSGMWFSGLALIFVLLTYGGWNE
                     AAYLTAEMRDTRRNIVRALVIGIVVITVLYVLLNLAYVRVLGLSGMQSSKAVASDFMK
                     VTWGEGGAWLLGVAVATAALTTLNATVFTGARTNYALGRDFVIFRALGRWNEKASAPV
                     NALLVQGGISLALVALASSTPDGFQTMVAYTAPAFWLFFLLTGISLFPLRRQVPVNID
                     PFRVPFYPVTPLLFAAACAYMLYSSFNYAMSLDPGSIGAMVGIAMLASGVPVLMWAKR
                     VAAESGRG"
     gene            complement(1399..2322)
                     /locus_tag="AC16CX_06300"
     CDS             complement(1399..2322)
                     /locus_tag="AC16CX_06300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3012161.1"
                     /translation="MKPAALMQGILALTVALLAPAAMAQEGRGDVVYVPTPQIVVDEM
                     LRMAKVGPNDYVIDLGSGDGRIVITAAKKHGARGFGVDLDTYLLKLANDNAKKEGVAD
                     RARFIEQNLFETDLGPATVITSYLLPEMNEKLRPKLFQLKPGTRVVAHDYALGEWDPD
                     QEKTLLVPEKVVGDPGKSYIFLYIVPARIAGRWESEIMAGAKPVLYQFNFEQKFQLVH
                     GAARVGERELRLPQFRLAGEELAFNLTVPGQGGAVSHRFRGRVKGDAIEGTVTVGEGG
                     GQRVMPWRARQTARGEVNMGASGAILMGALR"
     gene            complement(2429..3334)
                     /locus_tag="AC16CX_06305"
     CDS             complement(2429..3334)
                     /locus_tag="AC16CX_06305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3012162.1"
                     /translation="MTTHAANHTGRARAAVVLLAMAPALSPAADQHPRADVPYVPTPA
                     NVVDAMLRLANVGPQDFVIDLGSGDGRIVIAAAKKHGARGFGVDIDGSLVSAARREAR
                     SQGVAGRVEFREENLFITDIGRASVLTMYLYPRVLMQLRPRLMEELKPGTRVVSHDFD
                     MENWPPDARVTVPVPEKPYGPPSSEVYLWIVPANAAGTWRWRSTVGAAAVDYELTLSQ
                     TFQLLEGKPLVGGRPGRFEAGRMRGDEIHFMLTAEVSGRRLHHEFNGRVGGDAINGKV
                     RLEDSEFDWKATRVRRGSINTNGER"
     gene            complement(3331..4233)
                     /locus_tag="AC16CX_06310"
     CDS             complement(3331..4233)
                     /locus_tag="AC16CX_06310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3012163.1"
                     /translation="MSTKAKGERRKAEVKENNVNGCFSFLLSTFAFLLAVPLAAAQEA
                     PSTHPDVPYVPTPQVTVDEMLRLANVGPKDFVLDLGSGDGRIVITAAKKFGARGLGVD
                     LDFHLVIQSEESARQAGVEDRVKFLQQDLFKTDLSQATVITLYLLPRVVRKLRPRLLE
                     LKPGTRIVSHDFDLDDWKPDRKTTVRKNVFLWIVPAKIAGRWQTRLPLSPIERLLELE
                     LTQRHQEVNAQARLNGVPTQVWETKLEGDRLSFVVADTTDRDNEAGLYFEGRVSGDAI
                     EGEVTRGVGNAISTVKWRAVRTSR"
     gene            complement(4236..>4745)
                     /locus_tag="AC16CX_06315"
     CDS             complement(4236..>4745)
                     /locus_tag="AC16CX_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012164.1"
                     /translation="ADYRDATVVTLYLLPSLVEKLKPRLLSELRPGTRIVAHDYGFSG
                     WKPDRLVVISKTYHLYIVPARVAGKWRLQAALPDGARDYEFDLEQHYQEIRGGARVEG
                     GFLPAFEARLEGDRIAFVLVEDGASHRFEGRVRGGLEMEGVVRSGVGRTLTENRWRAT
                     RVISGAGEG"
BASE COUNT          804 a         1646 c         1497 g          798 t
ORIGIN      
        1 ctcgcggtcc gtatccagaa gacggtcagc ctcgcccgga ttctgccgcc acccgcttgg
       61 cccacatcaa caccggcacc ccggacgcga gcatcgcgat gccgaccatc gcgccgatcg
      121 agccgggatc gaggctcatg gcgtagttga aactcgagta cagcatgtag gcgcaggccg
      181 cggcgaacaa cagcggcgtc accggataga acggcacccg gaacggatca atgttgaccg
      241 gcacctgccg gcgcagcggg aagagcgaaa tcccagtcag caggaagaac agccagaacg
      301 ccggggcggt gtaggccacc atcgtctgga acccatccgg cgtggaagaa gcgagcgcga
      361 ccagtgcgag cgaaatcccg ccttggacaa gcagcgcgtt gaccggcgca gaagctttct
      421 cgttccaccg tcccagagcg cggaagatga cgaaatcgcg gcccagcgca tagttggtgc
      481 gcgcaccggt aaagaccgtc gcgttgaggg tggtcagcgc tgccgtcgcc actgcgacgc
      541 caagcagcca tgcgccgcct tctccccagg tgactttcat gaaatcggat gccaccgctt
      601 tcgacgactg catgccggag aggcccagca ccctgacgta cgccaggttg agcagcacat
      661 acagcacggt gattacgacg ataccgatca ccagcgcgcg cacgatgttg cgccgggtgt
      721 cgcgcatctc ggcggtgaga taggccgcct cgttccatcc gccgtaggtg agcagcacga
      781 aaatcaacgc cagcccggag aaccacattc cgcttttcgc ggggtccggc gcggcggcgg
      841 gcgccgggga aaccatgacc ccgccgacaa tgatggcgag aatggcggcc gcaagggcga
      901 ccgtgagcgc gttctgcacc cacttgctct cgctggttcc ggttgcattg agagcggtga
      961 tcgcgatcac cgccagcccg gcgtacagcg ccgagctgta ttctcccaat gaaaacaagg
     1021 tagtggtgta atcgccgacg acgtacgcga tggcggcgat cgccccggtc tggatcaccg
     1081 tcatgcgcgc ccaggcgaag agaaagccga gccagtcacc gtaggccttg ctcaggaagt
     1141 agtactcgcc gccagcgttg gggtgcgagc tgccgagctc cgcgtagcac agcgcgccga
     1201 ccagcgaaat cacgccgccc gccagccata cgccgatgaa cgccgtctcg cccgagacgt
     1261 tgccggccac gatcgacggc gccttgaaga tgccgacgcc gatgacgatg ccgacgatca
     1321 tcgccaccgc gtcgaacacg gaaagcgtct ggcgcggctg ccctcccgcg ccctgccgcg
     1381 gcgcctgcgc cggctcgctc accggagcgc acccatcagg attgcgccgg atgcgcccat
     1441 gttcacctcg ccgcgcgcgg tctgcctggc ccgccacggc atcacgcgct ggccgccgcc
     1501 ttcgccgact gtgaccgtgc cctcgatcgc atcacccttg acccgcccgc ggaagcgatg
     1561 gctgaccgcg ccgccctgcc ccggcacggt caggttgaag gcaagttctt cgcccgccag
     1621 ccggaactgc ggcaggcgca gttcccgctc gcccacgcgc gcggcgccgt gcacgagctg
     1681 gaacttctgc tcgaagttga actggtagag cacgggtttg gccccggcca tgatctcgga
     1741 ctcccagcgg ccggcgatcc gcgccggcac gatgtacaga aatatgtagc tcttgccggg
     1801 atcaccgacg accttctcgg ggacgagcag ggtcttctcc tggtcggggt cccactcgcc
     1861 cagggcgtag tcgtgcgcca ccacgcgcgt gccgggcttg agctgaaaca gtttgggccg
     1921 cagcttctcg ttcatctccg gcagcaggta gctggtgatc accgtggcgg ggcccaaatc
     1981 ggtctcgaac aggttctgct cgatgaaacg cgcgcggtcc gccacgcctt cctttttcgc
     2041 gttgtcgttg gcgagcttca gcaggtaggt atcgagatcc acgccgaagc cgcgcgcgcc
     2101 gtgcttcttg gcggcggtga tgacgatgcg cccgtcgcca gagccgaggt cgatgacgta
     2161 gtcgttgggg ccgaccttcg ccatgcgcag catctcgtcc accacgatct ggggcgtggg
     2221 cacgtaaacc acgtcgcccc gcccttcctg ggccatcgct gccggcgcga gcagcgcgac
     2281 cgttagcgcg agaattcctt gcatcagtgc tgctggtttc atcgtaactc cttctcttct
     2341 atttcaaatg gcaagccgta agcggtaagc cgtaagcggt aagctgggtt cgtgttttta
     2401 ggaagttccc ccgctcgccg ctcacggctt accgctcacc gttcgtgttg atgctgccgc
     2461 gccggacgcg tgtcgccttc cagtcgaact cgctgtcctc gagcctgact ttcccgttga
     2521 tcgcgtcgcc gccgacgcgt ccgttgaact catggtgcag ccgccgcccg ctcacctcag
     2581 cggtgagcat aaaatgaatc tcgtccccgc gcatcctgcc cgcctcgaag cgccccggcc
     2641 tgccgccgac gaggggcttg ccttccagca actggaacgt ctgggagagc gtgagctcgt
     2701 aatcaacggc agcagctccg accgtggagc gccagcgcca cgtgccagcg gcgttggcgg
     2761 gcacgatcca gagatagacc tcgctcgaag gcggcccgta cggcttttcc gggaccggca
     2821 cggtcacgcg cgcgtccggc ggccagttct ccatgtcgaa gtcgtgggaa acgacgcgcg
     2881 tgcccggctt gagttcctcc atgagccgcg ggcgcagctg catcaacacg cgggggtaaa
     2941 gatacatcgt caacacgctg gcgcgtccga tgtcggtgat gaagaggttc tcctcgcgga
     3001 attcgacgcg gcccgccacg ccctggctcc gcgcctcgcg gcgcgcggcg ctgacgaggc
     3061 tcccgtcgat gtccaccccg aagccgcgcg ctccgtgttt tttcgccgcg gcgatgacga
     3121 tgcgcccgtc gcccgagccg aggtcgatga cgaagtcctg cgggccgacg ttcgcgagcc
     3181 tgagcatcgc gtccacgacg ttggcgggcg tcggcacgta gggaacgtcg gcacggggat
     3241 gctggtcggc cgctggcgac agcgccgggg ccatcgcgag cagcaccacc gcagcgcggg
     3301 cccggccggt gtggttcgcg gcatgggtgg tcatcggctg gtcctcaccg cgcgccactt
     3361 gacggtgcta atcgcgttgc cgacgccgcg cgtgacttcg ccctcgattg cgtcgccgct
     3421 cacgcggccc tcgaaataca ggccggcctc attatcgcgg tcggtcgtgt ccgccaccac
     3481 gaaggacaga cggtcgccct cgagcttcgt ctcccacacc tgcgtcggca cgccgttcag
     3541 gcgcgcttgc gcgttcactt cctgatggcg ctgcgtcaat tccagctcga gcaggcgctc
     3601 gataggagac agcggaaggc gcgtctgcca gcgccccgca atcttggcgg gcacgatcca
     3661 caggaacacg tttttcctga cggtggtttt ccggtcgggt ttccagtcgt cgaggtcgaa
     3721 atcgtgcgac acgatgcgcg tgccgggctt gagttccagc agccgcggcc gcagcttgcg
     3781 gaccacgcgg ggcagcaggt acagggtaat cacggtggcc tggctcaagt ccgtcttgaa
     3841 caggtcctgt tgcagaaact tcacgcggtc ttccacgccc gcctgccgcg cgctttcctc
     3901 gctctggata acgagatgaa agtcgaggtc cacgcccagc ccgcgcgccc cgaacttctt
     3961 cgcggcggtg atgacgatgc gcccatcgcc cgagccgagg tccagcacaa agtccttcgg
     4021 ccctacgttg gcgagccgca acatctcatc caccgtcacc tgtggcgtcg ggacataggg
     4081 cacgtccggg tgcgtcgagg gtgcctcctg tgccgccgcc agcggaaccg caaggagaaa
     4141 ggcgaaagtc gaaaggagaa aggagaaaca accattgacg ttgttttcct tcacttccgc
     4201 ctttcgcctt tcgcctttcg ccttggtgct catggctatc cctcccccgc gccgctgatc
     4261 acgcgcgtcg cgcgccagcg gttctcggtc agcgtgcgcc ccacgccgga acgcaccacg
     4321 ccctccatct ccagcccgcc ccgcacccgg ccttcgaagc ggtggcttgc gccgtcttcc
     4381 accagcacga aggcgatgcg gtcgccctcc agccgcgcct cgaacgccgg cagaaatccg
     4441 ccttcgaccc gcgcgccgcc gcggatttcc tgataatgct gctcgaggtc gaactcgtaa
     4501 tcgcgcgcgc cgtcgggcaa ggcggcctgc agccgccatt tgcccgccac cctcgccgga
     4561 acgatgtaca ggtggtaggt cttggaaatc accaccaggc ggtcgggctt ccagccggag
     4621 aaaccgtaat cgtgagccac gatgcgcgtg cccggcctca gttcggaaag cagccgcggc
     4681 ttcagcttct ccaccagcga aggcaggagg tacagcgtga cgaccgtggc gtcgcggtag
     4741 tccgc
//
LOCUS       DBNGWZ010000213         4746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2919081, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000213 DBNGWZ010000000
VERSION     DBNGWZ010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2919081"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC16CX_06320"
     CDS             complement(<1..260)
                     /locus_tag="AC16CX_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_188564739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR3012165.1"
                     /translation="MEHRPRIYYTEAQKALMWDRWQKGESLHAIARLFGRHHPSIRGV
                     LAQSGGIRPASRRRFRLALTLAEREEISRSVVAGHTIRSMAVS"
     gene            complement(552..1436)
                     /locus_tag="AC16CX_06325"
     CDS             complement(552..1436)
                     /locus_tag="AC16CX_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3012166.1"
                     /translation="MYEGFERFEIQTTDPEVKIVGRVGGKGPAALLLHGNPLTHVHWH
                     LVAPQLAKDFTVVATDLRGYGDSGKPRGKEDHSNYSFRRMAQDQVDVMAHLGFKEFFV
                     AGHDRGARTAHRMALDHPDKVRKFASFDILPTHHVLTNISRGWALGSFHWFFMAQPYD
                     IPEKLIEGKEDYYILQKLTKMGIGKGGFSKETLAEYARVCTPENIHGVCEDYRAAITV
                     DFEMDKADFDAGRKVACPAAIYWGEKSHTEKYFDPRKAWPQYCANIARIRPLPCGHYP
                     AEQVPDEVYEELSEFFKK"
     gene            1602..2660
                     /locus_tag="AC16CX_06330"
     CDS             1602..2660
                     /locus_tag="AC16CX_06330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3012167.1"
                     /translation="MKLLVLPGDGIGPEITDATLSVLDRANAKFRLGLEWQMMDIGLT
                     SLKKQGSTLPDAVMEAARSAPGIILGPVDHLQYPLREQGGINPSGELRVKLDLYANIR
                     PAKSRLGVGLTGKPVDLVIFRECTEGFYADRNMVEGVGEFMPTEDVALSIRKVTARQC
                     RRIARVAFECAMKRRKKVTAVHKANVLRISDALFLREVRKVAKDYPDVELDEKIVDAM
                     AALLVRDPARFDVIVTGNMFGDILSDEASELSGSLGLAGSIMAGDDRCCAQAQHGSAP
                     DIAGQDKANPTSLILSAAMLLEWLASRHGRSELAQASKAIESAIDASLKNPASRTPDL
                     GGKLGTRAFAAHVAGSIG"
     gene            2839..4296
                     /locus_tag="AC16CX_06335"
     CDS             2839..4296
                     /locus_tag="AC16CX_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HIR3012168.1"
                     /translation="MDYSVFITSLQNTLGEHLPSILGALGILVVGWIVAVLGRAGTRR
                     LLALLSVNRRIKESTEQTLDVESGIAVGVFWVILLVTFLGVFNALHLELISNPFQVLV
                     TQIFGYLPRLLAGTLLILVAWVLATLLRALIARVLARTEWDDKLSKEAGMEPMSKNVG
                     NVLFWLVILMFIPAILGAYDLQGLLAPVQAMINKVLDALPNVFAAFVIGFVGWLVAKV
                     MRGLVANLLAAAGADKAGHSAGLDQSVYLSRVVGTLVFIFIFIPTLIAALDALKIEAV
                     SKPATDMLGQVLNAVPHIVAAGLILVVTWYVAKFASALLSRLLAGMGFDALPEKLGMG
                     QALSGQFKPSSLVGVVILFFAMLFATVEAANQLQFTQVREVVTTFIKFGGDVLLGGLI
                     LLVGFWLANLAHTAIMRTGTENAKGLARLAQVAIIGLVIAMGLRAMGIADDIVNLAFG
                     LTFGAIAVAVALSFGLGGREAAGRQMEHWLSKLRK"
BASE COUNT          852 a         1484 c         1473 g          937 t
ORIGIN      
        1 gatacggcca ttgaacggat cgtgtggcct gcgaccaccg aacgcgatat ctcttcacgt
       61 tctgccagcg ttagcgcaag gcggaatcgt cgacgcgacg caggccgtat cccgccactc
      121 tgcgccagga cccctcgtat cgatgggtgg tgtcgaccaa acagccgtgc aatggcatgg
      181 agagattcgc ctttctgcca gcgatcccac atcaatgcct tctgagcttc cgtgtaatag
      241 atcctcggtc tgtgctccat ttgcaacatc ctccctctct cgtcagagaa tagaatgttg
      301 cgatgaccga ttgaaaccgc aatcgaaagc ggacgattcg ccctcgaagt ctggcgtggt
      361 ggcgcagttc tccggcgacg tctgacggcc aagatgccca tggccggcaa gcggctctac
      421 ggcctcggct gaggccgagt gacgaggtgc gggtgaagat atccgggtga cgagataatg
      481 gtgacgaaaa ccggcgtaat aacaattgta cgtcacccat attcgtcacc tgtaatcgtc
      541 acccttattc gtcacttctt gaagaactcg gagagttctt catagacctc gtccggcacc
      601 tgctccgccg ggtagtgccc gcacggcagc gggcggatgc gcgcgatgtt cgcgcaatac
      661 tgcggccagg ccttgcgcgg gtcgaaatac ttttcggtgt ggcttttctc gccccaatag
      721 atcgcggccg ggcacgcgac cttgcgtccg gcatcgaagt ccgccttgtc catctcgaaa
      781 tccacggtga tcgccgcgcg gtagtcctcg cacacgccgt ggatgttctc cggcgtgcag
      841 acgcgcgcgt attccgccag cgtctccttc gagaagccgc ccttgccgat gcccatcttg
      901 gtgagcttct ggaggatgta gtagtcctcc ttgccctcga tcagcttctc gggaatgtcg
      961 tagggctgcg ccatgaagaa ccagtggaag gagccaagcg cccatccccg cgagatgttc
     1021 gtcagcacgt gatgggtcgg caggatgtcg aagctcgcga acttcctcac cttgtcggga
     1081 tggtcgagcg ccatgcggtg cgcggtgcgc gcgccgcggt catggcccgc gacgaaaaac
     1141 tccttgaaac ccaaatgagc catgacatcg acctggtcct gcgccatgcg ccggaatgaa
     1201 tagttgctgt ggtcttcctt cccgcgcggc ttgccgctgt cgccgtagcc gcggagatcg
     1261 gtcgcaacca ccgtgaagtc cttggcgagc tgcggcgcaa ccaggtgcca gtgcacgtgc
     1321 gtgagcggat tgccgtgcag cagcagcgcc gccggtccct tgccgccgac gcgcccgacg
     1381 attttcacct cgggatcggt ggtctggatc tcgaaacgtt cgaagccttc gtacatgaag
     1441 ccgcctctac agtggacagg aaatgcacga tgttacaatc gcgcatctaa cataacaaca
     1501 cgtgagcggt gagcagttag cggtgagcgg aaaattcctt gcgatgacat gtcgtcttcg
     1561 ctgatcaccc accgcttacg gcttacggct taccgctaac catgaagctc ctcgttcttc
     1621 ccggcgacgg catcggcccc gaaatcaccg acgcgacgct ttcagtgctc gaccgcgcga
     1681 acgcgaagtt caggctcggg ctcgagtggc agatgatgga catcgggctg accagcctca
     1741 agaagcaagg ctcgacgctg cccgacgccg taatggaggc cgcgcgtagc gcgccgggca
     1801 tcatcctggg tccggtggat cacctgcagt acccgctgcg cgagcaaggc ggcatcaacc
     1861 cctccgggga gttgcgcgtc aagctcgacc tctacgccaa catccgtccg gccaagtccc
     1921 gcctcggcgt ggggctcacc ggcaagccgg tggacctcgt catcttccgc gaatgcaccg
     1981 agggtttcta cgccgaccgc aacatggtcg agggcgtggg cgagttcatg cccaccgagg
     2041 acgtggcgct ttccatacgc aaggtcacgg cgcgccagtg ccggcggatc gcccgcgtcg
     2101 cgttcgaatg cgcgatgaag cggcgcaaaa aagtaaccgc cgtgcacaag gccaacgtgc
     2161 tcaggatctc ggacgcgttg ttcctgcgcg aagtgcgcaa ggtcgcgaag gattacccgg
     2221 atgtggagct cgacgagaaa atcgtggatg cgatggcggc gctgctcgtg cgcgaccccg
     2281 cccgcttcga cgtcatcgtc accggcaata tgttcggcga catcctatcc gacgaagcct
     2341 ccgagctctc gggcagcctc gggctggccg gctcgatcat ggcgggcgac gaccgctgtt
     2401 gcgcgcaggc ccagcacggc tcggcgcccg acatcgcggg acaggacaag gcgaacccga
     2461 cctcgctgat tctttcggcg gcgatgctgc tcgaatggct cgcctcccgt cacggacgct
     2521 ccgagctcgc gcaggcgtca aaagccatcg agtccgcaat cgacgcctcc ctcaagaacc
     2581 ccgcctcgcg cacgcccgac ctgggcggaa agcttggaac gcgcgcgttc gccgcgcacg
     2641 ttgcaggctc aatcggttag gaattttccg gtctgccggc cggaaaattc cttatccgtt
     2701 ccagttttca acggcttatc cgcccgtgat ggaatgacgc gctggcgtca tcccatgctg
     2761 ttatgctatt cgagtgccgc tccggggagc agcacgttat ccggttcggc ccgttcgaat
     2821 accaaaaagg agggcgtcat ggattactcg gtcttcatca cctctctaca gaacaccctg
     2881 ggcgagcacc tgccgagcat actgggggcg ctcggtattc tcgtcgtcgg ctggatcgtc
     2941 gccgtattgg ggcgcgccgg caccaggcga ctcctggcgc tgctctccgt caaccggcgc
     3001 atcaaggaaa gcaccgaaca gacgctcgac gtcgagtccg gcatcgcggt cggcgtgttc
     3061 tgggtgatcc tgctggtgac ctttctgggc gtgttcaacg cgctgcatct ggagctcatc
     3121 tcgaacccgt tccaggtgct ggtgacccag attttcggct atctgccgcg gctcctggcc
     3181 ggcaccttgc tgatcctggt cgcatgggtg ctcgccacgc tgctgcgcgc gctgatcgcg
     3241 cgggtgctcg ccaggaccga gtgggacgac aagctctcca aggaagccgg catggagccg
     3301 atgagcaaga acgtcgggaa cgtgctgttc tggctggtga tcctgatgtt catcccggcc
     3361 atactcggcg cctacgacct gcaggggttg cttgcgccgg tgcaggccat gatcaacaag
     3421 gtgctcgacg cgctgcccaa cgtgttcgcc gcgttcgtga tcggcttcgt cggctggctg
     3481 gtggcgaagg tgatgcgcgg gctggtcgcg aacctgctcg ccgccgccgg cgccgacaag
     3541 gcgggacaca gcgcggggct cgaccagtcg gtgtacttgt cgcgcgtggt gggaacgctg
     3601 gtgttcatct tcatcttcat cccgacgctg atcgcggcgc tcgacgcgct gaagatcgag
     3661 gcggtatcga agccggccac cgacatgctg gggcaggtcc tgaacgcggt gccgcacatc
     3721 gtcgcagccg gcctgatcct ggtcgtcacc tggtacgtcg cgaagttcgc ctccgccctg
     3781 ttgagccggc tgctggccgg catgggattc gacgccctgc ccgagaagct cggcatgggg
     3841 caggcgctga gcggccagtt caagccctcg tcgctggtcg gcgtggtgat cctgttcttc
     3901 gccatgctgt tcgcgacggt cgaggccgct aaccagcttc agttcaccca ggtgcgcgag
     3961 gtcgtcacca cgttcatcaa attcggcggc gacgtgctgc tcggcggcct gatcctgctg
     4021 gtcggcttct ggcttgccaa tctcgcccat accgcgatca tgcgcaccgg gaccgagaac
     4081 gcgaaagggc tcgcgcggct ggcccaggtc gcgatcatcg gcctggtgat tgcgatgggc
     4141 ttgcgcgcga tgggcatcgc cgatgacatc gtgaacctcg ccttcgggct caccttcggc
     4201 gcaatcgcgg tggcggtggc gctgtccttc ggactgggcg ggcgcgaagc cgcgggcagg
     4261 cagatggagc actggctctc gaagctgaga aagtgactcg tgactggtga ctcgtaattg
     4321 gtgtcttcct tgccccggcc ggggcaagga ccaccgcagc aagaaccgag ttacgacccg
     4381 cgagtcgcga gtctatacaa aagaacgcca cccttgggtg gcgttctttt ttgcgaataa
     4441 atggccgttc aacgcgtggc gacaacttgt tgattggcag ggatatcagg ccgtccacgc
     4501 ggcctatttc gatattcctg cgtgcttcgc agcctatcct ggcacattcc agcgctaact
     4561 attggaaaat aaagcgaatc gcaaattctc tgcagcccgt ttccagatta gacgacaatt
     4621 cggaagttca ctatacgttg cacgagccct gcttcgcagg gggagtgtac gcaagcgatg
     4681 agcgtgcgtt ataggaggaa tggaatgcac gctcagagga atcttccagg agcattttga
     4741 gcagta
//
LOCUS       DBNGWZ010000214        22347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_903633, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000214 DBNGWZ010000000
VERSION     DBNGWZ010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 22347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22347
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_903633"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /gene="murC"
                     /locus_tag="AC16CX_06340"
     CDS             <1..1350
                     /gene="murC"
                     /locus_tag="AC16CX_06340"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR3012169.1"
                     /translation="LLTLRYKVSGSDLVENASTRRLRQLGAKIHIGHDEAHIAGADAV
                     VVSSAVKDDNPEVAAARARRIPLVPRALMLAELMRLKQGIAVAGTHGKTTTTSLVASV
                     LAEGGLDPTFVIGGRLEAAGSHARLGAGEYIVVEADESDASFLYLQPTLAVVTNIDAD
                     HMETYDHSLDKLRQAFVDFLQRLPFYGVAVLCADDTNVRAIMPQLTKPVTTYGFGPDA
                     HVRALDVAANGTQMRFRAVHGGEDRGAGGELAVTLNLPGAHNVRNALAAIAVGLELGV
                     PEAKIAKALADFRGVGRRFQQYGKVRLAGGGSFTLVDDYGHHPAEMAATLAAARGAFP
                     KRRVLLAFQPHRYTRTRDCFEDFVAVLSMADALVLTEVYPAGEAPIVAADGRALARAV
                     RVQGKVEPVFVEKVADLPAAILAAARDCDVVLTMGAGSIGTVAAQLASRVRSVNGAA"
     gene            1547..2488
                     /gene="murB"
                     /locus_tag="AC16CX_06345"
     CDS             1547..2488
                     /gene="murB"
                     /locus_tag="AC16CX_06345"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3012170.1"
                     /translation="MAEAIESGKVHGKTMRGDLRLNEPMAHHTTWRVGGAAERAYFPV
                     DLDDLIVYLRALSAREPVFVTGLGSNLLVRDGGLQGTVIFTHWALRGIRLGKTGEGGG
                     AIHAEAGVASPKVARFAALHDLAGAEFLAGIPGTVGGALAMNAGCFGGATWDIVADVT
                     TVDRRGALRGRTREEYEIGYRDVRLKAEAPHSSEEWFVAATFRLQRGSGAESRRKITE
                     LLKRRIETQPLGEPNAGSVFRNPHGDYAARLIEACGLKGRVIGGAQISEKHANFIVNL
                     GDARATDIEALIELAERSVQEKFGIALEREVQIVGEA"
     gene            2542..3483
                     /locus_tag="AC16CX_06350"
     CDS             2542..3483
                     /locus_tag="AC16CX_06350"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR3012171.1"
                     /translation="MVAPDASRPTPHAFGKVAVLMGGRSAEREISLKSGTMVLNALKK
                     SGVDAHAFDPRDSSLEALKAQRFDRAFIALHGRFGEDGTVQGALEYLGIPYTGSGVMA
                     SALAMDKWRTKLLWQAAGLPTPPHELMTARTDPAGLAQRLGLPLMVKPAREGSSIGMT
                     KVTSVEKIAPAHELAARYDDVVIAERFIEGAELTAGILREETLPLIRLETPREFYDYE
                     AKYFADDTRYICPSGLPAATEQSVQRLALEAFRLLGCSGWGRVDAMLDRDGRPYLLEV
                     NTIPGMTDHSLVPIAARAKGISFEELVVRILETAHVG"
     gene            3523..4263
                     /locus_tag="AC16CX_06355"
     CDS             3523..4263
                     /locus_tag="AC16CX_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3012172.1"
                     /translation="MWDKPALMNAAASALYAVAALLVLAAAAMQAARLPAFALHEVRI
                     GGELRHVTRDQIEDVVRRELRDNFITLNLASARAAFERLPWVRNVNVRRHWPARLDVA
                     LEEHVPLARWASDALVNTHGEVFRAAYDGELPVFIGPDGAAREIAIQYRYFRHSLGTI
                     GETPVEVQVTPRRAWQVKLESGTTLALGRENIEARLARFVAVRDRTLGRLGRRIDYVD
                     LRYANGFAVRIPELRHQKAEPKRGRRTG"
     gene            4281..5519
                     /gene="ftsA"
                     /locus_tag="AC16CX_06360"
     CDS             4281..5519
                     /gene="ftsA"
                     /locus_tag="AC16CX_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR3012173.1"
                     /translation="MSKARDNKKLIVGLDIGTSKISAIVAEIRPDGGFEIIGMGNHPS
                     RGLKKGVVVNIETTVNAIQRALEEAELMADCKIHEVYTGIAGSHIRSFNSQGMVAVKD
                     KEVAQADIEHVVDTARAVQIPNDQQILHVLNQEFIIDGQEDVREPLGMSGVRLEVKVH
                     IVTGAVSAAQNIVKCVRRCGLEVRDLILQPLASATAVISDDEKDLGVALVDIGGGTTD
                     LVVFTHGAIRHTAVIPIAGDQITNDIAMALRTPTKDAEDIKERYGCALSQLADPQDMV
                     EVPGLGDRDPRQLSRKTLAEVIEPRVEELYSLLQTELRRSGYEELLSSGVVITGGSSV
                     MQGMVELGEEIFHMPVRVGHPNYNGALAETVRHPRHSTGVGLLLAGLQQHRSRELARM
                     NVSSFQQVLERMRSWFTGNF"
     gene            5563..6723
                     /gene="ftsZ"
                     /locus_tag="AC16CX_06365"
     CDS             5563..6723
                     /gene="ftsZ"
                     /locus_tag="AC16CX_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3012174.1"
                     /translation="MFEIVDAQAQEAVIKVIGVGGCGGNAVDHMIEKGVGGVEFISAN
                     TDAQALKRNQAKIQLQLGASITKGLGAGANPEVGRQAALEDRERVAETIAGADMLFLT
                     AGMGGGTGTGAAPVMAELAKELGILTVAVVTKPFAFEGKRQRIAQDGIDALSPLVDSL
                     IVVPNDKLMEVLGNQVTLDDAFKAANDVLHGAVAGIAEIISCPGMINVDFADVRTVMA
                     EMGMAMMGSARAAGADRARLAAEQAVACPLLEDVNIADARGVLVNISASKATFQLQEM
                     YDVMGTIKAFSAESAHPIIGTVYDDALGDQLRVTIIATGLGRPAVRQQKPLTVVTTKT
                     GTDNQPLEVDYGALEMPAVMRSKRNRDATVEAMRQSGVEMLDIPAFLRKQAD"
     gene            7027..7938
                     /gene="lpxC"
                     /locus_tag="AC16CX_06370"
     CDS             7027..7938
                     /gene="lpxC"
                     /locus_tag="AC16CX_06370"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR3012175.1"
                     /translation="MIQQRTLKNMIRATGVGLHTGAKVYMMLRPAAANTGIVFRRTDL
                     TPPVEIKGEPYAVGDTRLSSCLEQQGVKVSTVEHLMSALAGLGIDNAYVDLTAPEVPI
                     MDGSAGPFVFLLQSAGIEEQGAPKKFIRILKPVEIKEGDKWARFEPHNGFKLTMSIDF
                     AHPVFDKARQSVTVDFSTTSYIKEVSRARTFGFMQDVETMRAQGLALGGSLDNAIVMD
                     EYRVLNTDGLRYEDEFVKHKVLDAIGDLYLLGHPLIGAFSAHKTGHALNNRLLRQLIE
                     DKPSWELVSFERPEDAPAFLTWQPQPA"
     gene            8116..8301
                     /locus_tag="AC16CX_06375"
     CDS             8116..8301
                     /locus_tag="AC16CX_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012176.1"
                     /translation="MLVLRLVGILVLITIVGSIALYLFTRNRRYLAFAWRVFQFAFVF
                     LLVFLALYVLERLVLVM"
     gene            complement(8330..8776)
                     /locus_tag="AC16CX_06380"
     CDS             complement(8330..8776)
                     /locus_tag="AC16CX_06380"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR3012177.1"
                     /translation="MPVQKIGDVIATSGHLKALARKAQCLRNLQQLLFEAIPPALAAA
                     SRVTNLKSGTLVVLADNAAIAAKLRQLAPRLLLHIQKQRAEVTGIRVEVQVKTHKIKA
                     DDKITRRPLPPDAIKDFANLSDALPASPLKSALARLVARRRVKKPA"
     gene            8800..9717
                     /locus_tag="AC16CX_06385"
     CDS             8800..9717
                     /locus_tag="AC16CX_06385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3012178.1"
                     /translation="MNIILVSERLAKARTITLGLPQIVLLGLGMMVSVIAVAAALNYL
                     MLRVAADFNIPYLHTLLLSAQQEQHEKTRSYLRENLNAMAVKLGQMQAQLVRLDTLGE
                     RLAKLAGFKPQDLVFSELPGRGGAISSVSSQDLSLGDFTRQLDLLTRQLDDRGDKLGV
                     LESLFTLDSAKKKLVPTMLPVEGGWYSSNYGWRIDPFTGQRAFHEGIDVMAEQGTTIR
                     AAAGGVVVFSDLHPQYGNMIEIDHGNDLITRYAHASRRLVKIGDVVLRGAKIAEVGRT
                     GRATGTHLHFEVRQRGAPVNPVQFLRLPG"
     gene            9779..12598
                     /gene="secA"
                     /locus_tag="AC16CX_06390"
     CDS             9779..12598
                     /gene="secA"
                     /locus_tag="AC16CX_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3012179.1"
                     /translation="MIQTVLRKIFGSRNDRLLRQYMRQVRAINALEPQMEKLTDAELR
                     AKTDAFRARIRDRLAAIPDNADGDAERASRLEEERTAARRVAVEELLPEAFAVVREAG
                     RRTLNMRHFDVQLVGGIALHNGKIAEMRTGEGKTLVATLPAYLNSLAGQGVHIVTVND
                     YLASRDADWMGRIYRFLGLTVGVNLSQMEHSLKQQAYGADITYGTNNEFGFDYLRDNM
                     VYQTSERVQRGLHYAIVDEVDSILIDEARTPLIISGQAEDTTELYVRMNQVAPKLVRQ
                     KEENGPGDYWVDEKAHQVILSEQGHEHAESLLEKAGMLARDSSLYDPANIILMHHLNA
                     ALRAHTLFLRDQQYVVQNGEIVIVDEFTGRLMPGRRWSEGLHQAVEAKEGVEIQKENQ
                     TLATITFQNYFRMYRKLAGMTGTADTEAYEFQQIYGLETVVIPTHMPMIREDRMDQVY
                     RTAREKHQAIINDIKDCYERGQPTLVGTTSIENSELLSEMLKKEQLQHNVLNAKQHAR
                     EAEIVIQAGRPKMITIATNMAGRGTDIVLGGNPEPEVNRVMADEMLDDAAKKGTVEEI
                     RFNWQKLHNQVVAAGGLHIVGTERHESRRIDNQLRGRSGRQGDPGSSRFYLSLEDPLL
                     RIFASDRVAAIMERLKMPEGEAIEHRWVSRSIENAQRKVEARNFDIRKHLLEYDDVAN
                     DQRKVIYQQRNELLETSDISETLDAMRAGVIGGHVNQHIPPESMEEQWDAAGLERTLE
                     ADLRLKPPVQRWLKDEPELEQETLRARIIEAARSQYRDKIASVDPQAMRHYERAIMLQ
                     SLDTHWREHLAALDHLRQGIHLRGYAQKNPQQEYKREAFELFSMMLESIKAEVTKILM
                     TVQIRSAQELEAVEEPEAPKQVQYQHAEYAGGGDTALETEAEGEVAVAERPQPFVRPG
                     QKVGRNDPCPCGSGRKYKHCHGRLT"
     gene            12737..13975
                     /gene="argJ"
                     /locus_tag="AC16CX_06395"
     CDS             12737..13975
                     /gene="argJ"
                     /locus_tag="AC16CX_06395"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3012180.1"
                     /translation="MPVNLKPPDPKQLLPVKGVALGTAEAGIRKTGRKDLLVIKLEDG
                     ARVAGVFTQNRFCAAPVVVARQHLTLLDPDSSIRALVVNTGNANAGTGKEGIAAARQT
                     CSELAKLLDCSAAQVLPFSTGVIMEPLPAEKITAGLPACLADAREDNWLAAAQAIMTT
                     DTVPKAASRRVKVGATEVAVTGIAKGAGMIHPDMATLLGFIATEAKLSLPLARAMAAH
                     AANRSFNCITVDGDTSTNDAFMLIATGKADMTEIGDAGSTAYAELMEGVITVAAELAQ
                     AIIRDGEGATKFIIIRVEGGRREEECRKVAYAIAHSPLVKTAFFASDPNLGRILAAVG
                     YAGIADLAVDKVELYLDDVLVAKNGGRSPGYREADGARVMKRPEITVRVVLNRGPATA
                     TVWTCDLSHDYVTINAEYRT"
     gene            13972..14859
                     /locus_tag="AC16CX_06400"
     CDS             13972..14859
                     /locus_tag="AC16CX_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3012181.1"
                     /translation="MTDADKLIRQAEGLLTRLERLLPREAAATDWSASIAFRWRKHNG
                     VGHIEPVRHVHRIALRDLQGIDTQKELVEQNTRQFVDGHPANNVLLTGARGTGKSSLI
                     KALLNKYAPKGLRLIEVEKRDLVDLPDIVDRIGERPERFLLYCDDLSFEAGEAGFKAL
                     KVVLDGSIAAASDNCVIYATSNRRHLMPEFMQENLDYKHVGEEIHPSETSEEKVSLSE
                     RFGLWVSFYPFDQEDYLKIVAVWLEHFKAPGARSEQVQRAALQWSLQRGSRSGRVAWQ
                     FARDWAGKTRLKADGGTRK"
     gene            14856..15830
                     /locus_tag="AC16CX_06405"
     CDS             14856..15830
                     /locus_tag="AC16CX_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HIR3012182.1"
                     /translation="MSASRQPSAVSRQPSTRVEVAAAVILRDDGRFLLGQRPAGKVYA
                     GYWEFPGGKIEPNEAPLAALGRELREELGIGIERAYPWLTRDYDYAHAAVRLRFFRVV
                     KWAGALHGRENQRFAWQAPDAISVDPLLPANAPIVRALKLPPVYAISNAAELGEREFL
                     VRLDTALARGLKLLQVREKKMPAAELLRFAAEVVRRAHARGARVLVNGDAGIAQQAGA
                     DGVHLTSAQLEQLDRRPALELAGASCHDAEELARARTLGADFAVLGPVKPTPTHPGSA
                     GIGWDRVTALLKDCPLPVYALGGLRATDLETAWGCGAHGISMMRGAWS"
     gene            16561..16868
                     /locus_tag="AC16CX_06410"
                     /pseudo
     CDS             16561..16868
                     /locus_tag="AC16CX_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018050.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            16930..17223
                     /locus_tag="AC16CX_06415"
     CDS             16930..17223
                     /locus_tag="AC16CX_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3012183.1"
                     /translation="MREELIETARGLYDLGAIDAQTMREFDALRLPPLKAYRPADIKR
                     LRLKARASQAVFAAYLNTSVSTVQKWEIGEKKPGGPALKLLNIVERKGLKALA"
     gene            17476..17886
                     /locus_tag="AC16CX_06420"
     CDS             17476..17886
                     /locus_tag="AC16CX_06420"
                     /inference="COORDINATES: protein motif:HMM:NF019151.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GmrSD restriction endonuclease domain-containing
                     protein"
                     /protein_id="HIR3012184.1"
                     /translation="MDIGYGDRRVFLLLSLLYPEFDYSEPFHIDHVYPKGKLTERHLE
                     KRGIAPELAAQMAELRDCLANLQLLRGRPNQEKSNCDFDDWLKKKYPDKRNREIFLQG
                     NLLPTDLAYKYEDFARFVEGRERAMAQRIKELLC"
     gene            18380..18784
                     /locus_tag="AC16CX_06425"
     CDS             18380..18784
                     /locus_tag="AC16CX_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012185.1"
                     /translation="MARIRHIAIGTQDPEATARFYIEGLGLKEVGRLNSATAEGYYLS
                     DGHVNLAILKFKSDDPATTEGAPKYTGVHHFGVEVEDMAQARARIEKAGAVHRPYPGT
                     EEMEKRGNVEVKFSGPDGVTIDLSEHGWAGTK"
     gene            complement(18858..19847)
                     /locus_tag="AC16CX_06430"
     CDS             complement(18858..19847)
                     /locus_tag="AC16CX_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3012186.1"
                     /translation="MTDARVVFIHGVSQRVLKAITSLNPEGFTTIEVEGKTTPEDKQI
                     EAVKDADFILWFSAKLPDRVLRSAKKVRLVQGLSAGYDSINLKLLRELKIPCANNGGA
                     NSWAVADHAVLAMLALYRRLAAAERSTREGNWRKAINGTNTFELANKVVGILGFGNIG
                     QKVARRVQAFDASVQYYDKFPQPPGRERELNVKRVALDELFRTSDIVTCHAPLTNETR
                     HVVNRRHLALMKPTALLINTSRGPVVDEAALIEALQQKRIAGAGLDVFEQEPVDPGNP
                     LLKMENVVVSPHSAGTTWDTWFRRAEFAYGNMKRVWAGEAPLAVATDYDSENP"
     gene            19936..20976
                     /locus_tag="AC16CX_06435"
     CDS             19936..20976
                     /locus_tag="AC16CX_06435"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR3012187.1"
                     /translation="MTPFSRIAPLVILLAVMGAAQAQDRLPLVKVVATGGTIANTPSG
                     RLHAGEVAEAIPQLKQVARLEVEEVIRVGSSSIKVENWLTLARRVNEILAKEPEVKGV
                     VITHGSNTVEETGYFLSLTVKSDKPVVLTAAQRQFRTLSSDSPKNFLQAVRVAATDEA
                     RGKGALIVTNDVINAARDVTKTMTYRLETYSSHDLGVLGYVDEDRVTFYRLPLKQHTR
                     ATPFDVSRLQKLPRVDIIYTAVDTDGALIEAAVTHARAEGLVIAGFPTGSGTPAMDEA
                     VKRVAAQGIPVVMTNRGGIGRSTETAARVKEARPLIWGDNLTPQKARILLMLALTTTK
                     DPGEIQQLFERY"
     gene            complement(20973..21182)
                     /gene="yacG"
                     /locus_tag="AC16CX_06440"
     CDS             complement(20973..21182)
                     /gene="yacG"
                     /locus_tag="AC16CX_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR3012188.1"
                     /translation="MSSSRVVSCPTCGKPVEWKAANAWRPFCSERCKLIDLGAWATEK
                     YRVPVVEDKDQLEDAEPGDPPVQKT"
     gene            complement(21275..22030)
                     /gene="zapD"
                     /locus_tag="AC16CX_06445"
     CDS             complement(21275..22030)
                     /gene="zapD"
                     /locus_tag="AC16CX_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HIR3012189.1"
                     /translation="MISYEYPLSERVRTLLRLEDLFERIDYFLHKNDPHEHHVALLSI
                     FEILEVSGRADLKSDLLQELERQKHTLEALRENPDVSEEALDNVLWQIDQASSRLYQA
                     SGKIGQELRENEWLMSIRQRTSIPGGVCEFDLPSYHYWLQQGPDQHRHDLQLWISPFL
                     PMRDGIAIVLRLLRESGKTSSQIAYQGVYQQMMAGRLSQMLRIRLKREYHCVPEISAN
                     KYALNIRFTTQEGVRRPKAADADVEFELTFCNL"
     gene            complement(22148..>22347)
                     /locus_tag="AC16CX_06450"
     CDS             complement(22148..>22347)
                     /locus_tag="AC16CX_06450"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3012190.1"
                     /translation="RQLERAVRRSRISADETRAIMASQLSRAARLGHADDVLDNDGDM
                     EALERQVVQLHAKYLALARGA"
BASE COUNT         4039 a         7076 c         7542 g         3690 t
ORIGIN      
        1 ctgttgacgc tccgctacaa ggtgagcggc tccgatcttg ttgagaacgc ctcgacccgg
       61 cgactgcggc agctcggcgc caaaatccac atcggccacg acgaagcgca catcgccggg
      121 gcggacgccg tcgtggtctc gagcgccgtg aaggacgaca accccgaggt cgcggccgcg
      181 cgcgcgcgcc ggatcccgct ggtgccgcgc gcgctgatgc tcgctgagct gatgcggttg
      241 aagcagggca tcgcggtggc gggtacgcac ggcaagacca ccacgacgag cctggtggcg
      301 agcgtgctcg ccgagggcgg gctcgatccg acgttcgtga tcggcggccg gctcgaggcc
      361 gcgggctccc acgcgcggct cggcgccggc gagtacatcg tcgtcgaggc cgacgagtcc
      421 gacgcctcct tcctttacct gcagccgacg ctggcggtgg tcaccaacat cgacgccgac
      481 cacatggaaa cctatgacca ctcgctcgac aaactgcgcc aggcattcgt ggatttcctg
      541 cagcggctgc cgttctacgg ggtagcggtg ctgtgcgccg acgacaccaa cgtgcgcgcg
      601 atcatgccgc agctcaccaa accggtcacg acctacgggt tcggtcccga cgcgcacgtg
      661 cgcgccctcg acgtcgcggc caacggcacg caaatgcgct tccgcgcggt gcacggcggc
      721 gaagaccggg gggcgggggg cgagcttgcg gtcacgctca acctgccggg ggcgcacaac
      781 gtgcgcaacg cgctcgcggc gatcgcggtc ggcctcgagc tgggagtgcc ggaggcgaag
      841 atcgccaaag cgctcgccga tttcaggggc gtgggccgcc gcttccagca gtacggcaag
      901 gtgcgcctgg cgggcggggg cagcttcacg ctggtggacg actacggcca ccacccggcc
      961 gaaatggcgg cgacgctcgc cgccgcgcgc ggcgccttcc ccaagcgccg cgtcctgctc
     1021 gcgttccagc cgcaccgcta cacgcgcacg cgcgactgct tcgaggattt cgtggcggtg
     1081 ctgtctatgg cggatgcgct ggtgctgacc gaggtctatc cggcgggcga ggcgccgatc
     1141 gtcgcggcgg acgggcgcgc gctcgcgcgc gcggtgcggg tgcagggcaa ggtcgagccg
     1201 gtgttcgtgg aaaaggtcgc cgacctgccg gccgcgatac tcgccgccgc gcgcgactgc
     1261 gacgtggtgc tcacgatggg cgcgggatcg atcggcaccg tggcggcgca gctcgcgagc
     1321 cgggttcgca gcgtgaacgg cgcagcgtga acggcgaagg atgaaggatg aaggatgaag
     1381 gatgaggatc ggtactgcag cgaacggaca agcctataca ggtcatccct ggtcagtgac
     1441 cccggcccgc cgcgctgcat cgcgtcgggc gttcgccggc tcgaacgtgc gcaggcacgt
     1501 tcttcgaatt cccggctcac ccctcatccc tcatccttgg tttgggatgg cggaagcgat
     1561 tgagtccggg aaggtgcatg ggaagacgat gcggggcgat ttgagattga acgagccgat
     1621 ggcgcaccac acgacctggc gcgtaggcgg cgcggccgag cgcgcctact ttcccgtcga
     1681 tctggacgat ctcatcgtgt atctgcgcgc gttgtccgca cgcgagcccg tgttcgtgac
     1741 aggcctcggc agcaacctgc tggtgcgtga cgggggcctg cagggcacgg tgatcttcac
     1801 ccactgggcg ttgcgcggga tacgtctcgg gaagacgggc gaagggggtg gagcgatcca
     1861 tgccgaggcc ggcgtggcaa gcccgaaggt ggcccggttc gctgcccttc acgatctcgc
     1921 cggcgcggaa ttcctcgccg gcatccccgg tacggtggga ggagcactcg cgatgaacgc
     1981 aggctgcttc ggcggcgcga cctgggacat cgtcgcggac gtgaccacgg ttgatcgacg
     2041 cggtgcgttg cgcgggcgca cgcgggagga atacgagatc ggatatcgcg atgtccgcct
     2101 gaaggcggag gcccctcatt cctcggagga gtggttcgtg gccgcaacgt tccgcctcca
     2161 gcgcggatcc ggcgccgaat cccgcagaaa gatcacggag ctgctcaaac ggcgcatcga
     2221 gacgcaaccg ctcggcgagc ccaatgccgg ctcggtgttc cgcaaccccc acggcgatta
     2281 cgcagcgcgg ctgatcgagg cgtgcggatt gaaggggcgc gtcatcggcg gcgcgcagat
     2341 ctcggaaaag cacgcgaact tcatcgtcaa cctcggcgac gcgcgcgcca ccgacatcga
     2401 ggccctgatc gagctggcgg agcggtcggt gcaggagaaa ttcgggatcg cgctcgagcg
     2461 cgaagtgcaa attgttggtg aggcgtaagg agtgaggagt gaggcgggaa aacggacaag
     2521 cgtcgagcga cgaggtgccc catggttgcg cctgacgcct cacgcccaac gcctcacgct
     2581 tttgggaagg ttgccgtgtt gatgggcggc cggtctgctg agcgggaaat ttccctcaag
     2641 agcgggacga tggtgctcaa cgcgctgaag aaaagcgggg tggacgcgca cgccttcgac
     2701 ccgcgcgaca gcagcctgga ggcgctcaag gcccagcgct tcgaccgcgc gttcatcgcg
     2761 ctgcacgggc gcttcggcga ggacggcacg gtgcagggcg cgctcgaata tctcggcatc
     2821 ccctacaccg gcagcggcgt gatggcgagc gcgctcgcga tggacaagtg gcgcaccaag
     2881 ctgctctggc aggcggcggg cctgcccacg ccaccccatg agctcatgac cgcgcgcacc
     2941 gaccctgccg gcctcgcgca gcggctcgga ctgccgctga tggtgaagcc ggcgcgcgag
     3001 ggctcatcca tcggcatgac caaggtgacc tcggtcgaga aaatcgcccc cgcgcacgag
     3061 ctcgcggcgc gctatgacga cgtggtgatc gcggagcgct tcatcgaggg cgcggagctc
     3121 accgcaggca tcctgcggga ggagacgttg ccgctgatcc ggctcgagac cccgcgcgag
     3181 ttctatgact acgaggccaa gtacttcgcc gacgacacgc gctacatctg cccgtccgga
     3241 ctccccgcgg cgacggagca gtcggtccag cggttggcgc tggaagcgtt ccggctgctc
     3301 gggtgcagcg gctggggacg ggtggacgcg atgcttgatc gcgacggaag accgtacctg
     3361 ctggaggtca acaccatacc cggcatgacc gaccacagcc tggtgccgat agcggcgcgg
     3421 gcgaagggca tttcgtttga ggaactggtc gtgcggattc tggagacggc gcatgtcgga
     3481 taacttggaa cttgaaaccg ggaaccgggg acaggcgtag ccatgtggga taagccggcg
     3541 ctcatgaacg cggccgccag cgcgctctac gccgtcgcgg cgctgctcgt gctggccgcc
     3601 gccgcgatgc aggcggcccg gcttccggca ttcgcgttgc acgaagtgcg gattggcggg
     3661 gagttgaggc acgttacccg cgaccagatc gaggacgtcg tgcgccggga gctcagggac
     3721 aacttcatca cgctcaacct cgcttccgcg cgcgccgcgt tcgagcggtt gccgtgggtg
     3781 cgcaacgtga acgtgaggcg tcactggccg gcacggctgg acgtcgcgct ggaggagcat
     3841 gtaccgctcg cgcgctgggc cagcgacgcg ctcgtcaaca cgcacggcga agtgttccgg
     3901 gcggcgtacg acggggaact gccggttttc atcggtcccg acggcgccgc ccgggagatc
     3961 gccatccaat accgctattt ccgccacagc ctgggaacga tcggcgagac gccggttgag
     4021 gtgcaggtca cgccacgccg cgcctggcag gtgaagctcg aaagcgggac gacgctcgcg
     4081 ctcgggcgcg agaacatcga ggcgcgactt gcccgcttcg tggcggtccg cgaccgcacg
     4141 ctcgggcgcc tcggtcgccg gatcgattac gtggacctgc gctacgccaa cggtttcgcc
     4201 gtgcgcatac cggaactgcg ccatcagaag gcggagccga agcgcgggcg gcggacagga
     4261 tagcgggcag ggacggacgc atgagcaagg caagagacaa caagaagctg atcgtgggcc
     4321 tcgacatcgg cacctccaag atctccgcga tcgtcgcgga gatccggccg gatggcggtt
     4381 tcgagatcat cggcatgggc aaccacccct cgcgggggct caagaagggc gtggtggtca
     4441 acatcgagac cacggtcaac gccatccagc gcgcgttgga ggaggcggag ctgatggccg
     4501 actgcaagat ccacgaggtc tacaccggca tcgcgggcag ccacatccgc agcttcaact
     4561 cgcagggcat ggtcgcggtg aaggacaagg aggtcgcgca ggccgacatc gagcacgtcg
     4621 tggacaccgc gcgcgcggtg cagatcccca acgaccagca gattctccac gtgctcaacc
     4681 aggaattcat catcgacggg caggaagacg tgcgcgagcc cctcggcatg agcggcgtgc
     4741 gcctcgaggt caaggtgcac atcgtgaccg gggcggtctc cgcggcgcag aacatcgtca
     4801 aatgcgtgcg ccgctgcggg ctggaagtgc gcgacttgat cctgcagccg cttgcttccg
     4861 ccacggccgt gatttccgac gacgagaagg atctcggagt cgcgctggtc gacatcggcg
     4921 gcggcaccac cgatctcgtc gtgttcacgc acggggcgat ccgccacacc gcggtgatcc
     4981 cgatcgcggg cgaccagatc accaacgaca tcgccatggc gttgcggacc cccaccaagg
     5041 acgccgagga catcaaggag cgttacggat gcgcgctctc gcagctagcc gatccccagg
     5101 acatggtcga agtaccgggg cttggcgacc gcgacccccg gcagctctcg cgcaagacgc
     5161 tcgcggaggt gatcgagccg cgcgtcgagg agctctattc gctgttgcag accgagctgc
     5221 gcaggagcgg ctacgaggag ctgctctcct cgggcgtggt gatcactggc ggctccagcg
     5281 tgatgcaggg catggtggag ctgggtgagg agattttcca catgccggtg cgggtcgggc
     5341 acccgaacta caacggcgcg ctcgcggaga ccgtgcgcca tccgcgccac tcgacggggg
     5401 tgggcctgtt gctggcgggc ttgcagcagc accgcagccg cgagctcgcg cgcatgaacg
     5461 taagctcgtt ccagcaggtg ctggaaagga tgaggtcgtg gtttaccgga aatttctagg
     5521 tcttgtttct tgcattcgtt gactcgatga aaggaggctt acatgtttga gatagtggac
     5581 gcacaggcgc aggaagccgt catcaaggtg atcggcgtgg gcggctgcgg cggcaacgcg
     5641 gtcgaccaca tgatcgagaa gggcgtcggg ggggtggagt tcatttccgc caacaccgac
     5701 gcgcaggcgt tgaagcgcaa ccaggcgaaa atccagcttc agctcggcgc cagcatcacc
     5761 aaggggctgg gcgcgggggc gaaccccgag gtcgggcgcc aggcggcgct ggaggaccgc
     5821 gagcgcgtcg ccgagaccat cgccggcgcc gacatgctgt ttctgacggc gggcatgggc
     5881 ggcggcaccg gcaccggcgc ggcgccggtg atggccgagc tcgccaagga gctcggcatc
     5941 ctgacggtcg cggtggtcac caagccgttc gcgttcgaag gcaagcgtca gcgcatcgcg
     6001 caggacggca tcgatgcgct gtcgcccctc gtcgattcgc tgatcgtggt gcccaacgac
     6061 aagctgatgg aggtgctcgg caaccaggtg acgctcgacg atgcgttcaa ggccgccaac
     6121 gacgtgctgc acggggcggt cgccggcatc gcggagatca tcagctgccc gggcatgatc
     6181 aacgtggatt tcgccgacgt gcgcacggtg atggccgaga tgggcatggc gatgatgggc
     6241 tcggccaggg ccgcgggcgc cgaccgcgcg cggctcgcgg cggaacaggc ggtcgcgtgc
     6301 ccgctgctcg aagacgtcaa catcgcggat gcgcgcggcg tgctggtcaa catctcggcg
     6361 agcaaggcca ctttccagct gcaggagatg tacgatgtca tggggacgat caaggcgttc
     6421 tcggcggaat cggcgcaccc catcatcggc acggtgtacg acgacgcgct gggcgatcag
     6481 ctgcgtgtga cgatcatcgc gaccggcctc ggccggccgg cggtccggca gcagaagccg
     6541 ctcactgtcg tgacgaccaa gaccggcacc gacaaccagc cgctggaggt ggactacggc
     6601 gcgctcgaga tgccggcggt gatgcgctcc aagcgcaatc gcgacgcaac ggtcgaggcg
     6661 atgcggcagt cgggcgtcga gatgctcgac ataccggcgt tcctgcgcaa acaggctgat
     6721 taggcgcgcc ggaaggagga gcaggagcag gtgcccggca tggccgggtg cgacccgtag
     6781 gcgcgcaagc ggccgaccct ggggtgtttg ttgcttgccg atgacgcttc tgggaccgca
     6841 gcggtcagca gtcagcggcc agatcttagc cggtcgtgcg cggcattgcc gcctacgtat
     6901 cgggtagctg aaagctgatc gctgaaagct gactgcggta acgtcccgtt gcactttttt
     6961 tgcacggcaa catcggcgcg tgctaaactt gctgcgttaa gaacctgaaa attcagggag
     7021 ttttccttga tccagcagcg cacgctgaag aacatgatcc gcgccaccgg cgtggggctg
     7081 cataccggcg ccaaggttta catgatgctg cgcccggcgg cggccaatac cggcatcgtg
     7141 ttccggcgca ccgatctcac cccgccggtg gagatcaagg gcgaacccta cgcggtgggc
     7201 gacacgcggc tctcctcctg cctggaacag cagggagtca aggtgtccac cgtcgagcat
     7261 ctgatgtcgg cgctggcggg cctgggcatc gacaacgcct acgtggacct caccgccccc
     7321 gaagtcccga tcatggacgg cagcgccggc ccgttcgtgt tcctgctgca gtcggcgggg
     7381 atcgaggagc agggcgcgcc gaagaaattc atccgcatcc tgaagccggt ggaaatcaag
     7441 gaaggcgaca agtgggcgcg cttcgagccg cacaacggct tcaagctcac catgagcatc
     7501 gacttcgccc acccggtgtt cgacaaggcg cgccagtcgg tcaccgtgga tttctccacc
     7561 acctcctaca tcaaggaagt gagccgcgcg cgcaccttcg gcttcatgca ggacgtggag
     7621 acgatgcgcg cgcagggact ggcgctcggc ggcagcctcg ataacgcgat cgtgatggac
     7681 gaataccgcg tgctcaatac cgacggcctg cgctacgagg acgagttcgt caagcacaag
     7741 gtgctcgacg cgatcggcga tctctatcta ctcggccacc ccctgatcgg cgcgttttcc
     7801 gctcacaaga ccggccacgc gctcaacaac cggctgttgc ggcagctgat cgaggacaag
     7861 ccgtcctggg agttggtgag cttcgagcgc cccgaggacg cccccgcctt cctcacctgg
     7921 cagccccagc ccgcttagac cgtgcgccta ccggcttgcg ggttttaagt tcctggtttc
     7981 aggttccaag tcgaaacctt cgaatcgcaa gccattcgaa ccttgaacct gaaacttgcg
     8041 ctactttgca aagcgtaggc cttgtgaagt cgaggatcga ttccgcttcg cggcaccgtc
     8101 cttaatgggg cgcgcgtgtt agttctcagg ctggttggca tcctcgtcct catcaccatc
     8161 gtcggctcga tcgcgctgta tctgttcacc cgcaaccggc gttatctcgc ctttgcctgg
     8221 cgggtgttcc agtttgcttt cgtgttcttg ctggtttttc tggcgctgta cgtcctcgaa
     8281 cgcctggtgc tggtgatgtg agggctcttg accccggcgt ctatccaagt cacgctggtt
     8341 tttttacccg gcggcgtgcc accaggcgcg cgagtgcgga cttgagcggt gaggccggga
     8401 gcgcgtccga gaggtttgca aaatccttaa ttgcatcagg gggtaacggg cgcctcgtga
     8461 tcttatcatc agccttgatt ttgtgcgttt ttacttgcac ttccacgcgg attccagtaa
     8521 cctcggctcg ctgtttctga atatgcaata acaagcgcgg cgcgagctgc ctcaatttgg
     8581 ccgcaatcgc cgcgttatca gccaaaacaa caagggttcc cgatttcagg ttcgtgaccc
     8641 ggctcgcagc tgcgagcgca ggggggatgg cttcgaacag cagttgctga aggttcctca
     8701 ggcattgcgc cttgcgcgca agcgccttga ggtggccgga ggtggcgatg acatcgccga
     8761 tcttctggac aggcaccggg gggtgtcggg aggcagacga tgaatattat cctggtttcc
     8821 gaaaggctgg cgaaagcgag gacgatcacg ctggggctgc cgcagatcgt cttgctcggc
     8881 ctgggcatga tggtttccgt gatcgcggtt gcggccgcat tgaattacct gatgctgcgg
     8941 gttgctgcag actttaacat tccttacctg catacactgc tgctgtcagc ccagcaagag
     9001 cagcacgaga agacccggtc ctacctgcgg gagaacctga acgccatggc ggtcaagctt
     9061 ggccagatgc aggcgcagct ggtgcggctc gacacgctgg gcgagcggct cgccaagctc
     9121 gccggcttca agccgcagga cctggtgttc agcgagctgc cagggcgcgg cggcgccatc
     9181 tcctcggttt cgtcccagga cctttccctc ggcgacttca cgcggcagct cgatttgctg
     9241 acgcgtcagc tggacgaccg cggcgacaag ctgggcgtac tcgagtccct gttcacgctc
     9301 gacagcgcga agaagaagct cgttcccacc atgctgccgg tggagggcgg atggtattcc
     9361 tccaattacg gctggcgcat tgatcccttc accgggcagc gggcgtttca tgaaggcatc
     9421 gacgtcatgg ccgagcaggg cacgaccatc cgcgccgccg ccgggggcgt cgtcgtgttc
     9481 tcggacctcc acccgcagta tggtaatatg atcgaaatcg atcacggcaa cgacctgatc
     9541 acgcgttacg cgcacgcgtc aaggcgcttg gtgaagatcg gtgacgtggt gctacggggc
     9601 gccaagatcg cggaagtcgg caggaccggg cgcgcgaccg gcacgcacct gcacttcgag
     9661 gtgcggcagc gcggagcgcc ggtgaacccc gtgcagttcc tgcggttgcc cggctgattt
     9721 gagagaccgg aatttcgaag gggggtgggg cgtgtcccca cccctttttg tttagttgat
     9781 gatacaaacc gttctgagga agattttcgg cagccgcaac gaccggctgc tccggcagta
     9841 tatgcggcag gtgcgcgcga tcaacgcgct cgagccgcag atggaaaagc tgaccgacgc
     9901 cgagctgcgc gccaagaccg acgccttccg cgcccgcatt cgcgaccggc ttgcggccat
     9961 tcccgacaac gccgatggcg atgccgagcg cgcgagcaga ctcgaggagg aacggactgc
    10021 cgcccgccgc gtggcggtgg aggagctact gccggaggcg ttcgcggtgg tgcgcgaagc
    10081 gggcaggcgc acgctcaaca tgcgccattt cgacgtgcaa ctggtcggcg gcatcgcgct
    10141 gcacaacggc aagattgccg agatgcgcac cggcgagggc aagacgctgg tggcgaccct
    10201 ccccgcctac ttgaactccc tcgccggcca gggcgtgcat atcgtgaccg tcaacgatta
    10261 cctggcaagc cgcgacgccg actggatggg caggatctac cgctttctgg ggctcacggt
    10321 cggcgtgaac ctctcgcaga tggagcactc gctcaagcag caggcctacg gcgccgacat
    10381 cacctacggc accaacaacg agttcggctt cgattacctg cgcgacaaca tggtttacca
    10441 gacttcggag cgggtgcagc gcggactcca ctacgcgatc gtggacgagg tggactcgat
    10501 tctcatcgac gaggcgcgca ccccgctcat catctcgggg caggccgagg acaccaccga
    10561 gctctacgtg cggatgaacc aggttgcccc gaagctcgtg cgccagaaag aggagaacgg
    10621 gccgggcgac tactgggtgg acgagaaggc gcaccaggtg atcctgtccg agcagggcca
    10681 cgagcacgcc gagtcgctgc tcgagaaggc cgggatgctc gcgcgcgatt ccagcctcta
    10741 cgaccccgcc aacatcatcc tcatgcatca cctcaacgcg gcgctgcggg cccataccct
    10801 gttcctccgc gaccagcagt acgtggtgca gaacggcgag atcgtcatcg tggacgagtt
    10861 caccgggcgg ctcatgcccg ggcggcgctg gtcggaaggg ctgcaccagg cggtggaggc
    10921 caaggaaggc gtcgagatcc agaaggaaaa ccagacgctc gcgacgatca ccttccagaa
    10981 ctacttccgc atgtaccgca agctcgccgg catgaccggc acggcggaca ccgaggccta
    11041 cgagttccag cagatctacg ggctggagac ggtggtgatt cccacccaca tgccgatgat
    11101 ccgcgaggac cgcatggacc aggtgtaccg caccgcgcgc gagaagcacc aggcgatcat
    11161 caacgacatc aaggactgct acgagcgcgg ccagccgacg ctggtcggta ccacctcgat
    11221 cgagaattcc gagctgctct cggagatgct caagaaggag cagctccagc acaacgtgtt
    11281 gaacgccaag cagcacgcgc gcgaggcgga aatcgtgatc caggccggcc ggccgaagat
    11341 gatcaccatc gccaccaaca tggcgggacg cggcaccgac atcgtgctgg ggggcaaccc
    11401 cgagcccgag gtcaaccggg tcatggccga cgagatgctc gacgacgcgg cgaagaaggg
    11461 cacagtcgag gaaatccgct tcaactggca gaagctgcac aaccaggtgg tcgcagccgg
    11521 cggcctgcac atcgtcggca ccgagcggca cgaatcgcgc cgcatcgaca accagctgcg
    11581 cgggcgctca gggcgccagg gcgaccccgg ctcgagccgt ttctacctct cgctcgagga
    11641 tccgctgctg cggattttcg cttccgaccg cgtggccgcc atcatggagc ggctcaagat
    11701 gccggagggc gaggcgatcg agcaccgctg ggtgtcgcgg tcgatcgaga acgcgcaacg
    11761 caaggtcgag gcgcgcaact tcgacatccg caagcacctg ctcgagtacg acgacgtggc
    11821 gaacgaccag agaaaggtca tctaccagca gcgcaacgag ctgctggaga cgtccgatat
    11881 ctcggagacc ctcgacgcaa tgcgcgcggg cgtcatcggc ggccacgtga accagcacat
    11941 cccgccggag agcatggagg agcaatggga cgcggcgggg ctggagagga cgctggaggc
    12001 cgatctgcgc ctgaagccgc cggtccagcg ctggctcaag gacgagccgg aactcgagca
    12061 ggagacactg cgcgcgcgca tcatcgaggc ggcgcgcagc cagtaccggg acaagatcgc
    12121 cagcgtcgac ccgcaggcga tgcgccacta cgagcgcgcg atcatgctgc agagcctgga
    12181 cacgcactgg cgcgagcact tggccgcgct cgaccatctg cgccagggca tccacctgcg
    12241 cggttacgcc cagaagaacc cgcagcagga gtacaagcgg gaggcgttcg agctgttctc
    12301 gatgatgctg gagtcgatca aggccgaggt caccaagatc ctgatgacgg tgcagatccg
    12361 cagcgcccag gagctcgagg cggtggagga gcccgaggcg ccgaagcagg tccagtacca
    12421 gcacgcggag tacgcgggcg gcggcgacac cgcgctcgaa acggaggcgg aaggcgaggt
    12481 cgcggtggcc gaacggccgc agccgttcgt gcgccccggc cagaaggtag ggcgcaacga
    12541 cccgtgcccc tgcggatcgg gcaggaagta caagcactgc cacggccggc ttacctgaag
    12601 gcgaaaggcg aaaggcgaaa gtaacgacgc agatcagggc tgttcgggct tgctactttc
    12661 gcctttcgac ttccgacttt cgacttgtcc tttaacctat aatcgcggcg tccttgcctg
    12721 ctgacgccgc gtatccatgc ccgtcaactt gaaaccgccc gacccgaagc aactgctgcc
    12781 ggtcaaaggc gtcgccctgg gcacggccga ggcgggcatc cgcaagaccg ggcgcaagga
    12841 tctcctggtg atcaagctcg aggacggcgc gcgcgtggcg ggcgtgttca cgcagaaccg
    12901 cttctgcgcc gcgccggtgg tggtggcgcg ccagcacctc accctgctcg atcccgacag
    12961 ctcgatccgg gcgctcgtgg tgaacaccgg caacgccaac gcgggaaccg gcaaggaagg
    13021 aatcgccgcg gcgcgccaga cctgcagcga gctggcgaag ctgctggact gctcggcggc
    13081 gcaggtgctg ccgttttcga ccggcgtcat catggaaccg ctgccggcgg aaaaaatcac
    13141 cgccggactc cccgcgtgcc tggccgatgc ccgtgaggac aactggctcg ctgcggcgca
    13201 ggcgatcatg accaccgaca cggtgccgaa agccgcttcc cgccgcgtca aggtcggggc
    13261 tacggaggtc gccgtgaccg gcatcgccaa gggtgccggc atgatccatc ctgacatggc
    13321 caccctgctc ggcttcatcg ccaccgaggc gaagctgtcg ctgccgctcg cgcgcgcgat
    13381 ggccgcgcac gcggcgaacc gctcgttcaa ttgcatcacg gtggacggcg acacctcgac
    13441 caatgacgcc ttcatgttga ttgcgacggg caaggccgac atgacggaaa tcggtgacgc
    13501 gggaagcacg gcttacgcgg agctgatgga aggcgtcata acggtggccg ccgagctcgc
    13561 gcaggccatc atccgcgacg gcgagggcgc aacgaagttc atcatcatcc gcgtcgaagg
    13621 cggccgcagg gaagaggagt gccgcaaggt ggcctatgcc atcgcgcatt cgccgctggt
    13681 caagaccgcg ttcttcgcct cggatcccaa cctcggccgg attctggccg cagtcggcta
    13741 cgcggggatc gccgacctcg cggtggacaa ggtggagctc tatctcgacg acgtgctggt
    13801 tgcgaagaac ggcgggcgca gtccaggtta ccgcgaggcg gacggggccc gtgtcatgaa
    13861 gcggcccgag atcacggtgc gggtcgtgct gaaccggggt ccggcgacgg cgacggtatg
    13921 gacttgcgac ctgtcgcatg attatgtgac gattaacgcc gagtaccgca catgaccgac
    13981 gcggacaaac tgatcaggca agccgagggg ctgctcacgc ggctggagcg gctgctgccg
    14041 cgggaagcgg cggccaccga ctggagcgcc tcgatcgcgt tccgctggcg caagcacaac
    14101 ggcgtgggcc acatcgagcc ggtgcggcac gtgcaccgca tcgcgctgcg cgatctccag
    14161 ggcatagaca cccagaagga gctggtcgag cagaacacgc ggcagttcgt cgacgggcat
    14221 cccgccaaca acgtgctgct caccggggcg cgcggcaccg gcaagtcctc gctcatcaag
    14281 gcgctgctca acaagtacgc cccgaagggc ctgcggctga tcgaggtcga aaagcgggac
    14341 ctggtcgacc tgcccgacat cgtggaccgc atcggcgagc ggcccgagcg cttcctgctc
    14401 tattgcgacg acctctcgtt cgaggctggc gaggcgggat tcaaggcact gaaggtcgtg
    14461 ctcgacggct cgatcgccgc ggccagtgac aactgcgtta tctatgccac ctccaaccgg
    14521 cggcatctca tgccggagtt catgcaggag aacctcgact acaagcacgt cggcgaggag
    14581 atccacccga gcgagacgtc ggaggaaaag gtatcgctgt ccgagcgctt cggcctgtgg
    14641 gtgtcgttct atccgttcga ccaggaggac tatctcaaga tcgtcgctgt ctggctcgag
    14701 cacttcaagg cgccgggcgc ccgctccgag caagtgcagc gcgcggcgct gcaatggtcc
    14761 ctgcagcgcg gctcgcgcag cgggcgcgtg gcgtggcagt tcgcccgcga ctgggcgggg
    14821 aaaaccaggc tcaaagccga cggcggaacg cgtaagtgag cgccagccgt cagccgtcag
    14881 ccgtcagccg tcagccatct actcgcgtcg aagtcgcagc ggccgtgatc ctgcgcgacg
    14941 acggccggtt cctgctgggc cagcggccgg cgggcaaggt ctatgccggc tactgggagt
    15001 tccccggcgg caagatcgag ccgaatgagg cgccgctcgc ggcgctcggg cgcgagctgc
    15061 gcgaggagct cggcatcggg atcgaacgcg cctacccctg gctcacccgc gattacgact
    15121 atgcgcacgc cgcggtgcgg ctgcgctttt tccgggtggt caaatgggcg ggggcgctgc
    15181 acgggcgcga gaaccagcgc ttcgcgtggc aggcgcccga cgcgatttcg gtcgatcccc
    15241 tgctgcccgc caatgcgccg atcgtgcggg cgctcaagct gccgccggtt tacgcgatca
    15301 gcaacgccgc ggagctcggt gagcgggagt ttctcgtccg gctcgacacg gcgctggcgc
    15361 gcgggctcaa gctcctgcaa gtgcgcgaga aaaaaatgcc cgcggccgaa ttgctccgat
    15421 tcgcggccga agtggtgcgg cgggcccatg cccgaggcgc gcgggtgctg gtgaacggtg
    15481 atgccggcat agcgcagcag gccggcgccg acggcgtgca tctcacgtcg gcccagctcg
    15541 agcaactgga tcggcgcccc gcgctcgagc tggccggcgc ctcctgccac gatgcggagg
    15601 agctcgcgcg cgcgcggacg ctgggggcgg acttcgcggt gctgggtccg gtcaagccca
    15661 cgcccacgca ccccggctcg gccggaatcg gctgggatcg ggtgaccgcg ttattgaaag
    15721 attgtccgct gccggtgtat gcgctcggcg ggctcagggc cacggatctg gaaacggcgt
    15781 ggggttgtgg cgcgcacggc atcagcatga tgcggggggc gtggagctga ctcttgcgtc
    15841 agcgcagcgc ttttcgcggc cggcatgact gccagcctgt tactagcgcg actgcgtcgc
    15901 gcattgccgg ggggcggccc cgggcgccgg tgactttctc tttgctcgcc cataagagaa
    15961 agtgcaccaa agagaaaggg cgccccggtt cgccgccgaa aaccgggaca gacctggtgt
    16021 gtgggtctgt cccggatttt cggttcccct gcgctgctcg accggtccgg cggtctgcgc
    16081 aactcgcgca ccgcctcgac tcttgcacat tcatcaggcg cgaatttctt acgatccggc
    16141 ctgcgtacac gcggctcgcc cagtgctcga ccacgccacg gatcaagctt ggaagctctg
    16201 gtgacagggc atcggctaat gttattggaa tcgttggtgg accgtggctc cgcggctgct
    16261 cccgcaaacg ggcctgcggc taacgagtcg cagccgcacc ccttgcccgg tttgcgctgc
    16321 tcgggcggct ctcaggggcc ccaagatcaa gatccatcac acgtcacccg taagcggggc
    16381 tcagagccgc acgctactgc gtcaacgctc ggtgtccggg ggcgtttact ttctggagga
    16441 gcttgcgttc gaagaatcga atgggtcccg catcgcgacc agccactgct ttcgctaggg
    16501 ggcgacgcta agcccgtggg acactatatc acttagtggt ataattaccg gcatgaacat
    16561 atgcgggtct tcaagaccag ggttttcaat cgctgtgcaa ggcgggaagg tcttgcagac
    16621 gagaacctct gcaaagccgt tgccgaaatg cagaaaggcc tggtcgatgc gcaacttggc
    16681 ggtggcgttt acaagaagcg ggtggcgagg gcaagcgcgg gggttaccgg gtcattcttg
    16741 cgacgaacct ccgggagcgg tggtttttca tgtttggatt cgcgaaaaac gagcgtgata
    16801 acgttgacga gaaggaactg aagttgctga agcaactcgc ggctgccttg ctcggcatga
    16861 aggattagga cattgaggaa gccaagaagc gagacgagct agtggagatc gattgtggca
    16921 aacaggagac tgcgtgaaga gttgatagaa accgcgcggg gtctgtacga tctcggcgcg
    16981 atcgatgcgc agaccatgcg cgaatttgat gcgctgcggc tgccgccgct gaaggcctat
    17041 cgtcctgcgg acatcaagcg gctgcggctg aaggcgcggg ccagccaggc cgtgttcgcg
    17101 gcctacctca ataccagcgt ctcgaccgtg cagaagtggg agatcggcga gaagaagccg
    17161 ggcggcccgg cgctgaagct gctcaacatc gtggagcgca agggcctcaa ggccctcgcc
    17221 tgaatttccc gtcactcggg ctagtagccc gtgagggcgt gagggcgcga gagcgggagg
    17281 gggaaatccc tctcacgatc tcccgacttc ccgatctccc ggcttttcgc cctcccgatc
    17341 tcccggtttt tccatctcac gacctcacga cctcccgacc tcacgggttc agagtgctgc
    17401 tcgggcggcg aacaggggcc ccaatgtcaa caccagcggt gtaacccatg tgtatgaaca
    17461 caggcgtaac ccatattgga cataggctac ggcgaccgga gggttttcct gctcctcagt
    17521 ttgctttatc ccgagttcga ttattccgag ccattccata tcgaccatgt ctatcccaaa
    17581 gggaagctga ctgagcgtca tctggagaag cgcggcattg ctccggaact cgcggcgcaa
    17641 atggcagaac ttcgcgattg cctcgccaac ctacaattgt tgcgtggaag acccaatcag
    17701 gagaaatcga actgcgattt cgacgactgg ctgaagaaga aatacccgga taagcgcaac
    17761 cgggaaatct tcctgcaagg gaatttgctc ccgacagatc tcgcatacaa gtacgaagat
    17821 tttgctcgat tcgtggaggg gagggagcgg gctatggcac agagaatcaa agagctgctc
    17881 tgctgaatgg tccggcaata aacaaatacg caggttgttc ccatgtgcga cgcgtttggc
    17941 tttggggccc ctgttcgccg cccgagcaac gcagactggc ccggggtttc ggtcgagcac
    18001 tgggcgagcc tcgtttgcgt ttgttcctcc gctgggacat cacgcctgat gagcatgcat
    18061 tggcgcaggc agtgcgcgag ttgcgcagac cgccggtccg gtcgagtagc gcaggggacc
    18121 gctacgagct tgcgaggtag cggcggcgaa ccggggcgcc cttcttcttt cgtgagtttc
    18181 ttcttgggcg agcaagaaga aagtcaccgg gaccggggcc gccccccgca agtggcgacg
    18241 cagtcgccga caccgccttc cgcgtgccgg cgcttgaccg gtctcgttgg acgagcgcat
    18301 gatttttcgc agcgcccgtt gcgcgtttcg cgcagagggc aggtcgagtt tccgatcacc
    18361 cggaatacag aggcagacca tggcacgcat tcggcacatc gcgattggca cgcaggaccc
    18421 agaagccacg gcgcggttct acatcgaggg cctcggcctg aaggaggtgg gcaggctcaa
    18481 ctccgcgacc gcggagggtt actacctgag cgacggccac gtcaacctcg cgatcctgaa
    18541 attcaagtcc gacgaccccg ccaccacgga gggcgcgccc aagtacaccg gcgtccacca
    18601 cttcggtgtc gaagtcgagg acatggcgca ggcacgggcg cgaatcgaga aggcgggcgc
    18661 cgtgcaccgt ccctacccgg gaacggagga gatggaaaag cgcggcaacg tcgaggtcaa
    18721 gttctcgggt cccgacggcg tgacgatcga cctctccgag cacggttggg cgggcaccaa
    18781 gtagcgtcaa atccggacgc tttgcgccgg tttaactccg cgcgttcgaa cgggcgagtc
    18841 cctcgccgtt caacgcgcta cgggttttcg ctgtcgtagt cggttgcgac cgccaaggga
    18901 gcctcgcccg cccacacccg cttcatgttg ccgtacgcga actcggcgcg gcggaaccag
    18961 gtgtcccacg tcgtgcccgc gctgtgcggc gataccacca cgttctccat cttgaggagc
    19021 gggttgccgg gatcgactgg ctcctgctcg aagacgtcca ggccggcgcc ggcgatgcgt
    19081 ttctgctgca gcgcctcgat gagcgcggcc tcgtctacca ccggcccccg gctggtgttg
    19141 atgagcagcg ccgtgggctt catcagggcg aggtgcctgc ggttgacgac gtgacgcgtt
    19201 tcgttggtga gcggcgcgtg gcaggtcacg atatctgagg tgcggaacag ctcgtccagc
    19261 gccacccgct tcacgttgag ttctcgctcg cgcccgggcg gctgtggaaa tttgtcgtag
    19321 tactgcacgc ttgcgtcgaa ggcctggacg cgccgcgcga ccttctggcc gatgttcccg
    19381 aatcccagga tgcctaccac cttgttcgcc aattcgaacg tgttggtgcc gtttatcgcc
    19441 tttctccagt taccctcgcg cgtggatcgc tccgccgccg cgagccgccg gtagagagcc
    19501 agcatcgcca gaacggcgtg atcggccacc gcccaggagt tcgcaccgcc gttgttggcg
    19561 cacggtatct tgagctcgcg caggagcttc aagttgatgc tgtcgtagcc cgccgagagt
    19621 ccctgcacca gccggacctt cttggccgac cgcagtacac ggtcggggag ttttgcgctg
    19681 aaccacagga tgaaatcggc gtccttgacc gcttcgatct gcttgtcttc cggcgtcgtc
    19741 ttgccctcga cctcgatcgt ggtgaagccc tcgggattga gcgaagtgat cgccttgagg
    19801 acgcgctgtg aaaccccgtg tatgaatacg accctggcat ccgtcatggg cttgctccta
    19861 tactggaatt ggaccggtga cgtcagtcta acgtgccgcg ccgcccggtg cgctggccag
    19921 ccaacggagg agtcgatgac acctttcagc cgaatcgccc cgctcgtcat tctgcttgcc
    19981 gtcatgggcg ccgcccaagc ccaggacaga ctgcccctgg tgaaggtggt cgcgaccggc
    20041 ggcaccattg ccaacacgcc ctcggggcgg ctccacgccg gcgaggtcgc cgaggcgatc
    20101 ccgcagctca agcaggtggc gcggctggag gtggaggagg tgatccgcgt cggcagcagc
    20161 agcatcaagg tggagaactg gctgactctc gcccggcgcg tcaacgagat cctcgcgaag
    20221 gagcccgagg tcaagggcgt ggtgatcacc cacggctcga acaccgtgga ggagaccggc
    20281 tacttcctga gcctcacggt gaagagcgac aagcctgtgg tgctcaccgc cgcgcagcgc
    20341 cagttccgca cgctgagctc ggacagcccc aagaacttcc tgcaggcggt gcgcgtcgcc
    20401 gctaccgacg aggcccgggg aaagggcgcg ctgatcgtca caaacgacgt gatcaacgcc
    20461 gcgcgcgacg tcaccaaaac catgacctac cgcctggaga cctacagcag ccacgatctg
    20521 ggcgtgctcg gctacgtcga cgaagaccgc gtgaccttct accgcttgcc gctgaagcag
    20581 cacacccggg ctacaccgtt cgacgtctcg cgccttcaga agctgccacg ggtcgacatc
    20641 atctacaccg cggtggatac cgacggcgcg ctgatcgagg cggcggtgac ccatgcccgc
    20701 gccgagggac tcgtgatcgc ggggttcccc accggctcgg ggacgcccgc catggacgag
    20761 gcggtcaaac gggtcgcggc ccagggcatc ccggtcgtga tgaccaaccg tggcgggatc
    20821 ggccgttcca ctgagaccgc ggcgcgggtg aaggaagcgc gcccgctcat ctggggggac
    20881 aacctgacac cgcagaaagc ccgcatcctg ctgatgctcg cgctgacgac gacgaaggac
    20941 cccggggaaa tccagcagct cttcgagcgc tattaggtct tctgcacggg cggatcgccg
    21001 ggctccgcgt cttccagctg gtctttgtct tcgacgaccg gcacgcggta cttctcggtc
    21061 gcccacgccc cgaggtcgat cagcttgcag cgctcggagc agaatggacg ccaagcattc
    21121 gctgccttcc attcaacggg cttgccgcag gtggggcagg aaacaacgcg agaggatgac
    21181 atgacagtca atagcgctca gccgtcagct ttcagccgtc agtctgaatc atacgccgct
    21241 ctgtttgcga gctgagcgct gattgctgac ggctttagag attgcagaag gttagctcga
    21301 actcgacgtc ggcatcggca gccttcggcc gccgcacgcc ttcctgagtg gtgaagcgga
    21361 tgttgagcgc gtacttgttg gcgctgatct ccggcacgca gtgatactcg cgcttgagcc
    21421 ggatgcgcag catctgcgac aggcgcccgg ccatcatctg ctggtacacg ccctggtagg
    21481 cgatttgcga cgaggtcttg ccggactcgc gcagcagtct cagcacgatc gcgatcccgt
    21541 cccgcatcgg caggaatggc gatatccaga gctgcaggtc gtggcggtgc tgatccggcc
    21601 cctgctgcag ccagtagtgg tacgacggca ggtcgaattc gcacacgccg ccgggaatgc
    21661 tggtgcgctg gcggatgctc atcagccact cgttctcgcg cagctcctgc ccgatcttgc
    21721 ccgaggcctg gtagagacgc gatgaggcct ggtcgatctg ccacagcacg ttgtcgagcg
    21781 cctcctcgga cacgtcggga ttctcgcgca gcgcttcgag cgtgtgcttc tgccgctcca
    21841 gctcctgcag cagatcggac ttgaggtcgg cccgacccga cacctcgagt atctcgaaga
    21901 tcgagagcag ggcgacgtgg tgctcgtgcg gatcgttctt gtgcaggaag taatcgatgc
    21961 gttcgaacag gtcctcgagc cgcagcaacg tgcgcacgcg ctcgctcagt ggatactcgt
    22021 agctgatcac gttgcgaaga ccatcggaac cggccaaggc cctgttttgc ggccaagtat
    22081 tcaccaaaac ccgaaaaaaa taaaggcctt attggagttt ttcaaggctg cagtacgctt
    22141 ctgccggtca cgccccgcgc gcgagagcaa ggtacttggc gtgaagctgc acgacctggc
    22201 gctcgagcgc ctccatgtcg ccgtcattgt cgagcacgtc gtcggcgtgc ccgaggcgcg
    22261 cggcgcgcga cagctgcgag gccatgattg cgcgcgtttc gtcggcggag atccggctgc
    22321 gccgcacggc gcgctcgagc tggcgct
//
LOCUS       DBNGWZ010000215         3977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1532514, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000215 DBNGWZ010000000
VERSION     DBNGWZ010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1532514"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="AC16CX_06455"
     CDS             <1..319
                     /locus_tag="AC16CX_06455"
                     /inference="COORDINATES: protein motif:HMM:NF025066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3012191.1"
                     /translation="GAPIFQATVAAAMEYDVDVICTATSGRLMMKGVAEKLFVKEGSP
                     KSVYDFIKDAHEAGVKFYCCSPNLDLFNMKEGDLIPECEGVIGGAHLIEEVMESDCKV
                     LTY"
     gene            337..1092
                     /locus_tag="AC16CX_06460"
     CDS             337..1092
                     /locus_tag="AC16CX_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3012192.1"
                     /translation="MAHATGTRVQAHIGDPVSEAPPVPRSKLEEIFEDIQSDLRYDHE
                     LNGCLNCGICTATCPAAHYYDFSPREIVQLLWTENLEGIYDAMQEKIWACAQCYTCAA
                     RCPFGNSPGGLVMLMRETAIKHAMESAKSVLRPFSRVMLKLISTGNQLAPDMITRDHF
                     PDWGPNISKVDAPLKLLRQAIPMPTLNTTVTAWEVNLKTSIELYTIWEETGVLKQLET
                     VDENLFDVIQDIMDEKREDWKDFLADQQDEKDD"
     gene            1103..2458
                     /locus_tag="AC16CX_06465"
     CDS             1103..2458
                     /locus_tag="AC16CX_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3012193.1"
                     /translation="MDKQTTGNERFTGHGSEWRDANLSPTEARMATAWVEQKIDRRSM
                     LTNKDRVQDVRDIMWQLEKDGEIVVHRVSDEHEPVTVKTLYGWDKKIPTRRLWHHKSC
                     GQCGNIPGYPASLLWLMNRLGIEYLDETDQTSCTAWNYHGSGIGNLESLAAVFLRNFH
                     QAYVAAKAQGLPEAYFYPLVHCGTSFGNYKEVRSYLLLSAKLRERVRQILGKLGRLVD
                     GKLLIPEEVVHYSEWVHVMRHRIASLQEIDCSRIRATIHPACHVYKMVPEDAIYDDTI
                     LGGNRVAVSTGIMEALGAQVIDYSTWYDCCGFGFRHIISEREFTRSFAIDRKVRVAIE
                     EAHADVMVGHDTGCITTLDKNQWIGKAAGKAVDLPILADCQFAALVCGAHPYKIVQSH
                     WHASSTETLMAKLGIDWQAKKAEFVGYLEEVKAGRQENLYDPRKMITSGPGYIPIAKR
                     P"
     gene            2455..3510
                     /locus_tag="AC16CX_06470"
     CDS             2455..3510
                     /locus_tag="AC16CX_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3012194.1"
                     /translation="MTQPILIVGAGPAGLAAAHALATVGQKPILVEKERRLGGAPILS
                     GYAKLVPSGEWAKDAIGGMVSRVEGNPLVTVHRGCTVKEFGGGPGAFRAVLSSGSALD
                     VGAAILCTGFTHFDSVNKPEWGFGTFPDVVTTTQVEQMLSSGKGVRCPSDGRKPERVA
                     ILLCVGSRDRQIGREWCSKICCTVSCNIAMEIREELPDAHVYIYYMDIRTFGLYETNY
                     YWRSQEEFKVKYIKARIAEVTSDGKRLIIKGEDTLVKRPITIPFDLVVHAIGMDPNVD
                     NKSLSQTFGFELERHGFVAKGNSYGALGETSRPGVFVAGAATSPETIDDSIAQAKAAA
                     MSAYALVQHAAAPAAAA"
     gene            3507..>3977
                     /locus_tag="AC16CX_06475"
     CDS             3507..>3977
                     /locus_tag="AC16CX_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012195.1"
                     /translation="MTGAWMVRPAASHASSPAGLERPQLELNGPKLALAFETLVSGTE
                     EHGGVERYVEALKLKSELFRCALGAGKARGLELATFKALAALIAPVRRRAVAYLVSPS
                     FEQARARLACLLDGAEDTTTTDLRIAEFCAGFPADRQHRWVRDLAADVLHNVDPE"
BASE COUNT          758 a         1299 c         1289 g          631 t
ORIGIN      
        1 cggcgcgccg atcttccagg cgacagtcgc cgcagcgatg gaatacgacg tcgacgtcat
       61 ctgcaccgcc acctccggcc ggctgatgat gaagggcgtt gccgaaaagc tcttcgtcaa
      121 ggaaggctcg cctaagtcgg tctacgactt catcaaggac gcacacgagg cgggcgtcaa
      181 gttctattgc tgctccccca acctcgatct gttcaacatg aaagagggcg atctcatccc
      241 ggagtgcgag ggagtcatcg gcggcgccca tctcatcgaa gaagtgatgg aaagcgactg
      301 caaggtcctg acctactgat tgcgcgagct ccagcgatgg cacacgcaac aggcacccgc
      361 gtccaggccc acatcggcga tcccgtttcc gaggcgccgc cggtgccccg cagcaagctg
      421 gaggagatct tcgaggacat ccagtcggac ctgcgctacg accacgagct gaacgggtgt
      481 ttgaattgcg gcatctgcac cgctacctgt ccggcagcgc actactacga cttcagcccg
      541 cgcgagatcg tgcaactgct gtggaccgag aatctcgagg gcatctacga tgccatgcag
      601 gaaaagatct gggcgtgcgc ccagtgctac acctgcgcgg cgcgctgccc gttcggcaac
      661 tcgcccggcg gcctggtgat gctgatgcgc gagacggcga tcaagcacgc aatggagtcg
      721 gcgaagagcg tgctgcgtcc gttcagccgc gtcatgctga agctgatctc aaccggcaat
      781 cagctcgcgc cggacatgat cacccgcgac cactttcccg actggggccc gaacatctcc
      841 aaggtcgacg ccccgctcaa gctcctgcgc caggcgatcc cgatgcccac gctcaacacc
      901 accgtcacgg cgtgggaggt taacctcaag acctcgatcg agctgtacac gatctgggaa
      961 gagaccggcg tgctgaagca gctcgagacc gtcgacgaga acctgttcga cgtgattcag
     1021 gacatcatgg acgagaaacg cgaggactgg aaggacttcc tcgcggacca gcaagacgaa
     1081 aaggacgatt gaggaggcgc gtatggacaa gcaaaccacc gggaacgaac gcttcaccgg
     1141 ccacggatcc gagtggcgcg acgcgaatct ctcgcccacc gaggcgcgga tggcaaccgc
     1201 gtgggtcgag cagaagatcg accggcgctc catgctgacc aataaggatc gcgtacaaga
     1261 cgtccgcgac atcatgtggc agctggagaa ggacggcgag atcgtcgttc atcgggtctc
     1321 cgacgagcac gagccggtga cggtcaaaac gctctatggc tgggacaaga agattccaac
     1381 gcggcggctc tggcaccaca agtcgtgcgg gcagtgcggc aacatcccgg gatatcccgc
     1441 gtcgctcttg tggctgatga accggctcgg catcgagtac ctggacgaga ccgaccagac
     1501 ctcgtgcacc gcgtggaact accacggctc gggcatcggc aacctggaaa gcctggccgc
     1561 ggtgttcctg cgcaacttcc atcaggccta tgtggccgca aaggcgcaag ggttgcccga
     1621 ggcctatttc tatccgctcg tgcattgcgg cacctcgttc ggcaactaca aggaggtgcg
     1681 cagctacctg ctgctatcgg cgaagctgcg tgagcgcgtg cggcagatcc tcggcaagct
     1741 cgggcggctg gtggacggca agctcctcat ccccgaggag gtcgtccact actcggagtg
     1801 ggtccacgtg atgcgccatc gcatcgcgag cctgcaggaa atcgactgca gccggatccg
     1861 cgcgacgatc cacccggcct gccacgtcta caagatggtg ccggaagacg cgatttacga
     1921 cgacacgatc ctcggcggca atcgcgtggc ggtgtcgacc gggatcatgg aagcgctcgg
     1981 cgcccaggtg atcgactatt cgacctggta tgactgctgc ggcttcggct tccggcacat
     2041 catttccgag cgcgagttca cccgatcgtt tgccatcgac cgcaaggtgc gtgttgcgat
     2101 cgaagaggca cacgccgacg tgatggtcgg ccacgacacc ggctgcatca cgaccctcga
     2161 caagaatcaa tggatcggca aagccgccgg caaggcggtg gacctgccga ttctggccga
     2221 ctgccagttt gcagcgctgg tctgcggcgc ccatccgtac aagatcgtgc agtcgcactg
     2281 gcacgcctcc tccaccgaaa cgctgatggc gaagctcggc atcgactggc aggccaagaa
     2341 ggccgagttc gtgggctacc tcgaggaggt caaagccggc aggcaggaga acctctacga
     2401 cccgcgcaag atgatcacct ccggcccggg ctacattccg atcgcgaaac ggccgtgact
     2461 caaccgatcc tgatcgtcgg cgcggggccg gccgggctgg ccgccgcgca tgcgctggcc
     2521 accgtcggcc agaagcccat tctggtggaa aaggaacgac gcctgggcgg agcgccgatc
     2581 ctctcgggtt acgcgaagct ggtgccctcc ggcgagtggg cgaaggacgc gatcggcggc
     2641 atggtgagcc gcgtcgaagg caatccgctg gtgacggtcc atcgcggctg cacggtgaag
     2701 gagttcggcg gcgggcccgg ggcgttccgt gccgtgcttt ccagcgggtc cgccctggac
     2761 gtcggcgcgg ccatcctctg caccggcttc acccacttcg actcggtcaa caagccggaa
     2821 tggggcttcg gcacgtttcc ggacgtggtg acgacgaccc aggtcgagca gatgctctcc
     2881 tcgggcaagg gcgtgcgctg cccctcagac ggccgcaagc ccgagcgcgt ggcgatcctg
     2941 ctctgcgtcg gctcgcgcga ccggcagatc gggcgcgaat ggtgctccaa gatctgctgc
     3001 accgtgtcgt gcaacatcgc gatggaaatc cgcgaggagc tgccggacgc gcacgtctac
     3061 atctactaca tggacatccg caccttcggc ttgtacgaga cgaattacta ctggcgcagc
     3121 caggaggagt tcaaggtcaa gtacatcaag gcgcgcatcg cggaggtcac ctccgacggc
     3181 aagcggctga tcatcaaggg cgaggacacg ctggtgaagc gcccgatcac gatcccgttc
     3241 gacctggtgg tgcacgcgat cggcatggac cccaacgtcg acaacaaatc gctcagccag
     3301 accttcggct tcgagctcga gcgccacggc ttcgtcgcca agggaaattc gtacggcgcg
     3361 ctgggcgaga cctcccgtcc gggcgtcttc gtcgccggtg ccgcgaccag tccggaaacg
     3421 atcgacgatt ccatcgccca ggcaaaggcc gctgccatgt ccgcctacgc gctggtgcag
     3481 cacgcggcgg cacccgcggc ggcggcatga ccggagcgtg gatggttcgc ccggcggcat
     3541 cgcacgcgtc gagcccggcg gggctggagc ggccgcagct tgagctgaac gggcccaagc
     3601 tcgcgctggc gttcgagacg ctggtctcgg gcaccgagga gcacggcgga gtggagcgtt
     3661 acgtcgaggc gctcaagctc aagtcggagc tgttccggtg cgcgctcggc gcgggcaagg
     3721 cgcgcggcct cgagcttgcg acgttcaagg cgctcgccgc cctgatcgcg ccggtgcgcc
     3781 ggcgcgcagt cgcctatctc gtcagtccct cgttcgagca ggcgcgcgcg cgcctcgcct
     3841 gcctgctcga cggcgccgag gacaccacga ccaccgacct gcgcatcgcc gaattctgcg
     3901 ccggatttcc cgcggaccgg cagcatcgct gggtgcgcga tctcgcggcc gacgtcctgc
     3961 acaacgtcga cccggag
//
LOCUS       DBNGWZ010000216         2567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2415725, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000216 DBNGWZ010000000
VERSION     DBNGWZ010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2415725"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC16CX_06480"
     CDS             complement(<1..812)
                     /locus_tag="AC16CX_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012196.1"
                     /translation="MLSATRLLACLICLVAPVAAWGQGAAAGYPARPVRVVVPFAPGG
                     SSDVVGRLTANKLVEFWGQQFVIDNRGGAGGAVGAETAARAAPDGYTLLFANQGPIVH
                     NVLLRRNLSYSWSDFAPVIYIGYTANLIVANPKFPPNNARELVAYARAHPGRINWASP
                     GTNSNPHIALEALKAAAGIDIVHVPYKGAGPALTDLVAGQVDAQYASFASSEPHVRSG
                     RLKVLAVSGTRRHPALPEVPTLAEQGVKGADSTLWFGYLVPARTPRALIDRL"
     gene            complement(825..1817)
                     /locus_tag="AC16CX_06485"
     CDS             complement(825..1817)
                     /locus_tag="AC16CX_06485"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012197.1"
                     /translation="MKIRGWFYFRLSTFTFLLCAIANLAIAQEWKPSRNVEIIVASGA
                     GGAADRSARQLQRLLQAGSDVPSITVSNRPGGGGLVAWTALAQHAGDAHYFATLSTAL
                     VTNQILGVGKLRYQDLTPLNILMREYVVAWVKADLPFASMRDVVARIRKDPASVSFAF
                     SSARGNQNHIVIGMIARAAGVDPKAVKTVVYSSGGQGMMTALGGHVDVWVGTPGGVLP
                     HLQTGAARALGISAGERQPGKLAAVPTFREQGINATYYAWRGFVAPGGLTPQQVAFWD
                     QAFAKAVRGEEWKRDLEDNGWAEDFRASAETRKHLDAEYELLAKMLADLGMVAR"
     gene            complement(1862..>2567)
                     /locus_tag="AC16CX_06490"
     CDS             complement(1862..>2567)
                     /locus_tag="AC16CX_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012198.1"
                     /translation="MDIYMMCETDAAVPLLLDAVKPRMAGAVLPERVPLPPIPGEVSL
                     RGVGAALETATRGIDVCYTRLPLGWSGAYTHFRHPLDYLGHDGGAGVGSGPAVTIGAG
                     LALKGSGRMPIALMGDGDFLMGNTAVWTAVHYQIPCLMIVCNNRSFYNDERHQGRMAR
                     ARGRPEENKWIGQHLTNPDINIAGMARMMGAEGIGPVDKVAEVLPALERGIAAVREGK
                     VCVIDARVLPGYDRGA"
BASE COUNT          396 a          915 c          847 g          409 t
ORIGIN      
        1 ttcagcctgt cgatcagcgc gcgcggtgtc ctggctggaa caagatagcc gaaccagagc
       61 gtggaatcgg cgcccttgac gccctgctcg gcgagggtcg ggacctccgg cagcgcggga
      121 tgccgcctgg tgccggacac cgccagcact ttcagccggc cggagcgcac gtggggctcg
      181 gaagaagcaa acgacgcgta ctgcgcgtcc acctggccgg cgaccaggtc cgtcagcgcc
      241 gggccggcgc ccttgtacgg gacgtgcacg atgtcgatgc cagccgcggc cttcagcgcc
      301 tcgagcgcga tgtggggatt gctgttcgtc cccggggaag cccagttgat cctgcccgga
      361 tgcgccctcg cgtacgccac cagctcgcgc gcgttgttcg gcgggaactt cgggttggcg
      421 acgatcagat tggccgtgta cccgatgtag atgacaggcg cgaaatcgct ccagctgtac
      481 gacaggttcc tgcgcagcag cacgttgtgc acgatcgggc cctggttggc gaacaggagc
      541 gtgtaaccgt cgggcgcggc ccgcgcggcg gtctcggcgc cgaccgcgcc accggcgccg
      601 ccgcggttgt cgatgacgaa ctgctggccc cagaactcaa cgagcttgtt cgccgtcaga
      661 cggccgacga cgtcgctcga gcccccgggg gcgaacggga ccaccactcg caccggcctc
      721 gcgggatagc cggccgcggc accctgtccc cacgctgcga ccggcgcaac gaggcagatg
      781 aggcaggcga gcaggcgcgt ggccgaaagc atcgggcctc cgtcttatcg cgccaccatg
      841 cccagatcgg cgagcatctt cgccagcaac tcgtactcgg cgtcgaggtg cttgcgcgtc
      901 tcggcgctcg cgcggaaatc ctcggcccac ccattgtctt cgagatcccg cttccactcc
      961 tcgccgcgga ccgcctttgc gaacgcctgg tcccagaacg cgacttgctg cggggtcagc
     1021 cccccgggcg cgacgaaccc gcgccaggcg tagtaggtgg cgttgattcc ctgctcgcgg
     1081 aacgtcggca ccgccgccaa ttttcccggc tggcgctcac ccgcgctgat tcccagcgcg
     1141 cgcgccgcgc cggtctgcag atgcggcagc acgccgccgg gggtcccgac ccacacatcg
     1201 acgtgtccgc cgagcgccgt catcatgccc tggccgcctg acgaatagac cacggtcttc
     1261 accgccttcg ggtccacgcc cgccgcgcgc gcgatcatgc cgatcacgat gtgattctga
     1321 ttgccgcggg cgctggaaaa agcgaatgac acggaggccg gatccttccg aatgcgcgcg
     1381 acaacgtccc tcatcgaggc aaacggcaaa tcggccttga cccaggccac cacgtactcg
     1441 cgcatcagga tgttgagcgg cgtgaggtcc tggtagcgga gcttgcccac gccgaggatc
     1501 tgattggtca ccagggccgt ggagagcgtg gcgaagtaat gggcgtcgcc tgcgtgctgc
     1561 gcgagcgcgg tccacgccac gagcccgccg ccgccgggcc ggttgctcac ggtgatgctg
     1621 ggaacgtcgg aacccgcctg caggagcctc tgcagttgcc gcgccgagcg atccgcggcc
     1681 ccgcccgcgc cggaggccac gatgatttcc acgttccggc tcggcttcca ctcctgcgcg
     1741 atcgcgaggt tcgcgatcgc gcaaaggaga aaggtgaaag tcgaaaggcg aaagtaaaac
     1801 caacccctga ttttcacgat tttccctttt gcctttcgac tttcgccttc cgccttgctt
     1861 ttcaggcgcc gcggtcgtag ccgggcagca cgcgcgcgtc gatcacgcag accttgcctt
     1921 cgcggaccgc cgcgatgccg cgctcgagcg ccggcagcac ttcagcgacc ttgtccaccg
     1981 ggccgatgcc ctcggcgccc atcatgcgcg ccatgcccgc gatgttgata tccgggttgg
     2041 tgaggtgctg gccgatccac ttgttttctt ccggccggcc gcgtgccctg gccatgcgcc
     2101 cctggtggcg ctcgtcgttg tagaaggagc ggttgttgca cacgatcatc aggcagggaa
     2161 tctggtagtg cacggccgtc cagaccgccg tattccccat caggaaatca ccgtcgccca
     2221 tcaacgcgat cggcatgcgg ccgctcccct tcaacgcgag gcccgcgccg atcgtcacgg
     2281 ccggccccga gcccacgccg gcgccgccgt cgtgcccgag gtagtcgagc gggtggcgga
     2341 agtgggtgta ggcaccgctc cagccgagcg gcagccgcgt gtagcagacg tcgatcccgc
     2401 gcgtggcggt ttccagcgcc gcgcccacgc cgcgcagcga gacttcgccg gggatcggcg
     2461 gcagcggcac gcgctcgggc agcaccgcgc ccgccatgcg cggcttgacc gcgtcgagca
     2521 gcagcggcac cgcggcgtcg gtctcgcaca tcatgtagat atccatc
//
LOCUS       DBNGWZ010000217         7292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1532860, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000217 DBNGWZ010000000
VERSION     DBNGWZ010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1532860"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2679)
                     /gene="glnE"
                     /locus_tag="AC16CX_06495"
     CDS             complement(<1..2679)
                     /gene="glnE"
                     /locus_tag="AC16CX_06495"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR3012199.1"
                     /translation="MYSHNAPASLSDDALIARVVRLSHYARRLVEATPSSGLDAGVGR
                     PFSGEEMRAALAADPGNNEENLKRALRVLRARVMLRLMARDLGGLAPLGEVLATNTAL
                     AEVAVSHALGRLDEWLALRHGRPIGAESGREQQLHVLGMGKLGGAELNVSSDIDLVFV
                     YPEEGETVGPRPISNHEYFTRLGRQLIAALSEITADGYVFRVDMRLRPYGDGGPLVAG
                     FDALEDYLITQGREWERYAWIKARPLTGDRGAELMELARPFVYRRHLDYNALASLRDL
                     HRQIRHEVERRDLFDNIKLGPGGIREIEFIAQVFQLIRGGRDPDLRKQPTLAVLPLLA
                     KRNLLPEPAVEELTEAYRFLRGLEHRLQYRDDQQTHTLPQAEADRVLIAETMGFTGYD
                     ALAGELQRHRGRVTLHFESIFATSPAGEHTLAYMWEEPDEGERMLSELAALGFRRPQA
                     IRERLRSMRAGGRYREMPAESQAQLDRLVPLAIEAAAAQPEPDATFDRVLDLLDSISR
                     RGSYLALLEEYPQALARLVSMMSASPWVAQYLTRHPILLDELLDTRTLYAAPDWAAAR
                     DHLRVQLDDARGDAEKQMDLLRHFKHANTLRFVAQDLAGLLPLETLADRLSELADLIL
                     EETIRVCWASLLSRHRERPVFAVVGYGKLGGKELGYASDLDLIFLYEDAAAEAPEAYA
                     RLAQRINHWLTSLTSAGILYETDLRLRPDGASGMLVSPVEAFEDYQFKHAWVWEHQAL
                     TRGRFCAGDAQIGAKFEDIRRAVLQQPRDLGELGREIIAMRKKMHDAHPNRSGLFDLK
                     HDAGGIVDVEFTVQYLVLGHAHHHPELTRNIGNLALLKLAGELGLIPEALAEDVRSAY
                     REFRRRQHMLRLAGEKYARANPAEVRAYADSVRALWQ"
     gene            2748..6578
                     /locus_tag="AC16CX_06500"
     CDS             2748..6578
                     /locus_tag="AC16CX_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR3012200.1"
                     /translation="MALQGKLRWLGIGSLWAYRVLTWSVLAAGLVFAASVLALRYWIL
                     PNIENYREDIARIVSERARQKITIGRIHANWEGLRPRLVLEQVTVYDATGRAALELSR
                     VDNTLSWLSLPTLELRFYALDIHRPTLNIRRDARGVISIAGIELTGETGEGGFADWLL
                     RQRDIEVFDATLVWNDESRGAPQLELKNVFLHVFSRGGRHRFGLRAVPPRELAAPIDV
                     RGDLRGDTVKSLADWDGRLFLQLDYADIAAWRTWVPFPVEFPRGSGALRAWLSFSHHQ
                     LVDVLADVKLANVRTRLAKDLPEMDLTELAGRIGWKQSDAGFEVTTSRLGLTTTGGLT
                     LHPANLLLRATSSNARRQGRGEMQANALELAPLTALADHLPLAQEVRKQLAAYSPKGS
                     LYDMVVRWTGDWREPQQYSVRGRFQNLSLNRVGKIPGFTGVSGTLEGNERGGTLSLNS
                     QRATVEMPLVFRDTLEFEALTAQVAWARSGKETELRLNGISFSNPHLAGTVFGNYRTA
                     GPARGSIDLTGKLTRADARFVTRYIPLVMGKSAREWMDKAFIAGQSNDVTLRLKGDLD
                     KFPFPDGEGGLFQVAAKITGGVLHYADGWPNIENIAGDLIFRGKRMDVHARQGALFGA
                     RLAKVHVEIPSLLEGQEMLSVTGEAEGPTGDFLAFIENSPVLGMIDHFTEGWQARGVG
                     KLSLKLSIPLRATAKSRVAGTYQFANNTVVIAPELPAVEQAGGQVEFTDSAVRVQNVS
                     GVVLGGPVAIAATTSRDATVRVTLQGRVNTDNVRRAGGPSWVQHMRGSTDWRAVLTAR
                     KRTADFVIESNLQGLAVDLPAPVVKAASESMPLRFERRVLAANQDRLSLSIGEIASMN
                     LVRRSEDGRATITRGAVRFGGTAVEPDRNGVWLSGTVKALDVDRWLALLGQGGETRIE
                     WGGLDMKLGSMDVFRRRFNDLAVNVAVQGGQWRGTLAGKELEGSVSWLPQGRGRLVAR
                     MKTLAIPPAAPGAVAPEESVLRSKKDLELPALDIVAEQFINKSKQLGRLELAAVSEGR
                     DWRIEKLRIINPESTFTLDGTWQLGLAQPRTQVNLRLEASDAGKLLTRLGYPEGVRRG
                     TSRLEGTLAWNGAPYDLDYPSLSGNLVLEAAKGQFLKLEPGIAKLLGILSLQALPRRV
                     TLDFRDVFSEGFSFDEIVGAVKINHGIASTENFRIQGPPARVAMSGEVDLAQETQKLR
                     VRITPHVSDTVSIAGAIVGGPVAGVAAFLAQKVLKDPLDQIVSYEYSVSGTWAEPNVK
                     RVEKPAPDAEKAQQ"
     gene            6575..6904
                     /locus_tag="AC16CX_06505"
     CDS             6575..6904
                     /locus_tag="AC16CX_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012201.1"
                     /translation="MIRLLLSVLLYAAALAAAAQTFVVPSELWDRPRSGRAVLEQPAI
                     RQAVNACLAQPGARLVVRHGAGQESQLAAEELRSWLAALAVEPGRISLRGDLPSSQPL
                     QLEVIRD"
     gene            7006..>7292
                     /locus_tag="AC16CX_06510"
     CDS             7006..>7292
                     /locus_tag="AC16CX_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3012202.1"
                     /translation="MNAARSSFGRARPHVAKSGGQPAVTRIAAVQLASGPNIKGNLHE
                     TGRLVEMAVAQGAKLVALPEYFAIMGLRDQDKVKLREKPGAGPIQEFLSTTA"
BASE COUNT         1205 a         2391 c         2486 g         1210 t
ORIGIN      
        1 ctgccacagc gcccgcactg agtcggcata tgcgcggacc tccgccgggt tcgcgcgtgc
       61 gtatttttcc ccggcgagcc gcagcatgtg ctgcctgcgg cggaattccc gataggcgct
      121 ccggacgtcc tccgcgagcg cttccggtat gaggccgagt tctcccgcga gcttgagcag
      181 tgccaggttg ccgatgttgc gggtgagttc ggggtgatgg tgcgcgtggc ccagcaccag
      241 atactgcacg gtgaactcga cgtcgacgat gccgcccgca tcatgcttga gatcgaacag
      301 cccgctgcgg ttggggtggg cgtcgtgcat cttcttgcgc atcgcgatga tttcgcggcc
      361 cagctcgccg aggtcgcgcg gctgctgcag caccgcgcgg cgtatgtctt caaacttcgc
      421 cccgatctgc gcgtcccccg cgcagaaacg gccgcgcgtc aacgcctgat gttcccatac
      481 ccaggcgtgc ttgaactggt aatcctcgaa cgcttcgacc ggactgacca gcatgccgct
      541 cgcgccgtcc ggcctgagcc tcaagtccgt ttcatagagg atgccggcgg acgtcaggct
      601 ggtcagccag tggttgatgc gctgcgcgag gcgcgcgtag gcttcaggcg cctccgccgc
      661 cgcgtcctca taaagaaaaa tcagatcgag gtccgaggca tagccgagtt ccttgccgcc
      721 gagcttgccg taaccgacga cagcgaacac cggccgctca cggtgccggc tgagcaggct
      781 cgcccagcac acgcggatcg tttcctcgag aatcaggtcc gccagttcgc tcaggcgatc
      841 ggcgagcgtt tcgagcggca gcagacccgc gaggtcctgc gccacgaagc gcagagtgtt
      901 ggcgtgcttg aagtgcctca gcaggtccat ctgcttctcg gcgtcacccc gcgcatcgtc
      961 gagttgcacg cgcagatggt cccgcgccgc ggcccagtcc ggcgctgcgt agagcgtgcg
     1021 ggtatcaagc agttcgtcga gcaatatcgg gtgccgggta aggtactggg ccacccacgg
     1081 gctcgcgctc atcatcgaca caagccgggc gagcgcctgc ggatactcct cgagcagggc
     1141 aagataggaa ccccggcggc tgatgctgtc gagcaggtcg agcacccgat cgaacgttgc
     1201 atccggctcc ggttgggccg cggcggcctc gatcgcgagc ggcaccagcc ggtcgagctg
     1261 cgcctggctc tccgcgggca tctcgcggta gcggccgccg gcgcgcatgc ttctcagccg
     1321 ctcacgtatc gcctgcggcc ggcggaatcc gagcgccgcg agctccgaga gcatcctttc
     1381 gccctcatcc ggctcttccc acatgtaggc gagcgtgtgc tcgccggcag gggacgtggc
     1441 gaagatcgac tcgaagtgca gcgtgacgcg accgcggtgg cgttgcaact cgccggcgag
     1501 tgcgtcgtag cccgtgaaac ccatggtctc ggcgatgaga acgcggtcgg cttccgcctg
     1561 cggcagcgtg tgcgtctgct gatcatcgcg gtattgcagg cggtgctcga gaccgcgcag
     1621 gaagcggtac gcctccgtga gctcttctac cgccggctcc ggcaggagat tgcgcttcgc
     1681 aagcaatggc agcaccgcaa gcgtgggctg ctttctcaga tcgggatcgc ggccgccgcg
     1741 gatgagctgg aacacctgcg cgatgaactc gatctcgcgg atgccgcctg ggcccagctt
     1801 gatattgtcg aagaggtcgc ggcgctcgac ctcgtgccgg atctggcgat gcaggtcgcg
     1861 cagcgaggcg agggcgttgt agtcgagatg gcggcggtag acgaacggac gcgcgagctc
     1921 catcagctcg gccccacggt caccggtgag cggtcgcgcc ttgatccagg cgtaacgctc
     1981 ccactcccga ccctgggtga tgaggtagtc ctccagcgcg tcgaaacctg caacgagcgg
     2041 accgccgtcg ccgtatggcc gcaaccgcat gtccacgcgg aacacgtagc cgtccgccgt
     2101 gatctcggag agcgctgcga tcagctgccg cccgagccgc gtgaagtatt cgtgattgct
     2161 gatcgggcgc ggccccaccg tttccccttc ctccggatag acgaagacga ggtcgatgtc
     2221 ggaggaaacg ttgagttcgg cgccgccgag cttgcccatg ccgaggacgt gcagctgctg
     2281 ctcgcgtccg ctctccgcac cgatcggcct tccgtgccgt aaagcgagcc attcgtccag
     2341 gcggccaagc gcgtggctca ccgcgacctc ggcgagcgcg gtgttggtcg ccagcacttc
     2401 gccgagcggc gcgagcccgc ccaggtcgcg cgccatcaaa cgcagcatca cgcgcgcgcg
     2461 caacacgcgc agcgcgcgtt tgagattttc ctcgttattg ccgggatcag ccgcgagcgc
     2521 cgcgcgcatt tcctcgccgc tgaacggacg cccgaccccg gcatccaacc ctgagctggg
     2581 cgtggcttcg acgagacggc gtgcgtagtg actcagccgc accacgcgcg cgatcagcgc
     2641 gtcgtcggaa agcgacgcag gagcgttatg ggaatacatg gacgattgcg ggtaaaatac
     2701 gtaagccctt gactcaatat cgcatttttt cgggactcag gcaaaacgtg gcgcttcagg
     2761 gcaagctcag atggctgggc atcggctcgc tctgggctta tcgcgtcctc acctggagcg
     2821 tgctcgccgc cggtctggta ttcgccgcga gcgtgctcgc cctgcgttac tggattctgc
     2881 ccaacattga gaattaccgc gaggacatcg cccgcatcgt cagcgaacga gcgcggcaga
     2941 agatcacgat cggcaggatc cacgccaact gggaagggct gcgcccccgg ctcgtgctcg
     3001 agcaggtcac ggtgtacgac gccacggggc gggcggcgct cgaactttcg cgcgttgaca
     3061 ataccctctc ctggctatca ttgcccacgc tcgagttgcg gttttacgcg ctcgacatcc
     3121 atcggcccac gctcaacatc cggcgcgacg cgcgcggggt gatttcgatc gccggcatcg
     3181 aattgaccgg cgagacgggg gagggcggct tcgccgactg gctgctgcgc cagcgcgaca
     3241 tcgaggtgtt tgacgccacc ctcgtctgga acgacgagag ccgcggcgcg ccgcaactgg
     3301 agctgaagaa cgtctttctg cacgtgttca gccgcggcgg ccggcatcgt ttcgggttgc
     3361 gcgccgtgcc gccgcgcgag ctggcggcgc cgatcgacgt gcgcggggac ctgaggggcg
     3421 ataccgtgaa gtctctggcg gactgggacg gccggctgtt cctgcagctc gactacgccg
     3481 acatcgccgc ctggcgcacc tgggtgccgt tcccagtcga gttcccccgc gggagcggcg
     3541 cgctgcgcgc gtggctctct ttcagccatc atcagctggt cgatgtcctt gcggacgtga
     3601 agctcgccaa cgtgcgcacc cggctcgcca aggacctgcc cgagatggac ctcacggagc
     3661 ttgcggggcg catcggctgg aagcagtccg acgcgggatt cgaggtcacc acttcaaggc
     3721 tcggcctcac caccaccggc ggtctcacgc tgcacccggc gaatctcctg ctgcgggcca
     3781 cctcgagcaa tgcgcgcagg caggggcgcg gggaaatgca ggccaacgcc cttgagcttg
     3841 cgccgctgac agcgctcgcc gaccacctcc cgctcgcgca ggaagtgcgc aagcaactgg
     3901 cggcgtactc gccgaagggc agcttgtacg acatggtggt gcggtggacg ggggactggc
     3961 gggaaccgca gcagtatagc gtccgtggac ggtttcagaa cctgtcgctc aaccgcgtcg
     4021 ggaagatacc ggggtttacg ggcgtgagcg gcacgctgga gggcaacgag cggggcggca
     4081 cgctctctct caactcgcaa agggcgacgg tggagatgcc gctggtattc cgcgatacgc
     4141 tcgaattcga ggccctcacg gcgcaggtcg catgggcgcg cagcgggaag gagaccgagc
     4201 tcaggctcaa cggcatctcg ttctccaacc cgcacctcgc cggcaccgtg ttcgggaatt
     4261 accgcaccgc gggcccggcg cgcggcagca tcgatctcac cggcaaactt actcgggcgg
     4321 atgcgcgttt cgtcacccgc tacattccgc tggtgatggg caagagcgcg cgtgaatgga
     4381 tggataaggc gttcatcgcg ggccagtcga acgacgtgac gctgcgtctc aaaggcgatc
     4441 tcgacaagtt tcccttcccc gacggcgagg gcgggctgtt ccaggtggcg gcgaagatca
     4501 ccgggggcgt gctgcactat gcggacggct ggccaaacat cgaaaacatc gccggcgatc
     4561 tcatctttcg aggcaagcgc atggacgtgc acgcgcggca gggcgccttg ttcggggcgc
     4621 gactcgcgaa ggtgcacgtc gagatcccgt ctttgctgga ggggcaagag atgctcagtg
     4681 tcaccggcga agcggagggg ccgaccggcg atttcctggc tttcatcgag aacagccccg
     4741 ttctcggcat gatcgaccat ttcaccgagg gctggcaggc gcgaggcgtc gggaagctgt
     4801 cgctgaagct ctcaataccc ctgcgcgcga ccgcaaaaag cagggtcgcc ggcacctacc
     4861 agttcgccaa caataccgtc gtcatcgccc ccgagcttcc cgccgtggag caggcgggcg
     4921 gtcaggtcga gttcaccgac tcggccgtgc gcgtgcaaaa cgtgagcggg gtggtgctcg
     4981 gcgggccggt ggccattgcc gcgacgacat cgcgcgatgc cacggtgcgg gtcaccctgc
     5041 agggacgcgt gaacacggac aatgtgcgac gcgccggcgg tccgtcgtgg gttcagcaca
     5101 tgcgtggttc caccgactgg cgcgcggtgc tcaccgcgcg caagcgcact gcggacttcg
     5161 tgatcgaatc gaacctgcag ggccttgccg tcgatttgcc ggcgccggtc gtgaaggccg
     5221 catccgaatc gatgccactg cggttcgagc gccgcgtgct ggcggcgaat caggaccggc
     5281 tgagcctgtc catcggcgag atcgcgagca tgaacctcgt gcgtcggtcc gaggacgggc
     5341 gcgccacgat cacgcgcggc gcggtgcgct tcggcggcac cgccgtcgaa cccgatcgca
     5401 acggcgtctg gttgagcggg accgtgaagg ctctggatgt cgaccgctgg ctcgcgctgt
     5461 tgggacaggg tggcgagacg cgcatcgaat ggggcggcct ggacatgaag ctcggatcga
     5521 tggacgtatt ccgcaggcgc ttcaacgatc ttgcggtgaa cgtcgccgtc cagggcgggc
     5581 aatggcgcgg cacccttgcc ggcaaggaac tggagggaag cgtgagctgg ctgccgcagg
     5641 ggcgcggccg gctcgtggcc cgtatgaaaa cgctcgcaat cccgccggcc gctccgggcg
     5701 ccgtggcgcc ggaggagagc gttctgcggt cgaagaagga cctcgagctg ccggcgctcg
     5761 acatcgtcgc cgagcaattc atcaacaaga gcaagcaact cggccggctc gagctcgcag
     5821 ccgtgtccga gggccgcgat tggcgcatcg agaagctgcg catcatcaat cccgagagca
     5881 cgttcacgct cgacggcaca tggcagctcg ggcttgccca gccgcgcacc caggtcaacc
     5941 tgaggctgga ggcgtccgat gccgggaaac tcctcacccg cctcggttat cccgagggcg
     6001 tgcggcgcgg cacctcgagg ctcgagggca cgctcgcctg gaacggcgcg ccgtacgacc
     6061 tcgactatcc gtcgctctcc ggcaacctgg tgctggaggc cgccaagggc cagttcctca
     6121 agctcgagcc gggcatcgcc aagctcctcg gcatcctcag cctgcaggcg ctgccccggc
     6181 gcgtcacgct cgatttccgc gatgtgttca gcgaaggctt ttccttcgac gagatcgtcg
     6241 gagcggtcaa gatcaaccac ggcattgcga gcacggagaa cttccgtatc caggggccgc
     6301 ccgcgcgggt cgcgatgagc ggggaggtgg acctcgcgca ggaaacgcag aagctgaggg
     6361 tgcggattac cccgcacgtg agcgacaccg tgtccatcgc gggcgccatc gtcggcggcc
     6421 cggtcgccgg cgtggcggcc tttctcgcac agaaagtgct gaaggacccg ctcgaccaga
     6481 tcgtctccta cgagtacagc gtgagcggca cctgggcgga accgaacgtg aagcgggtcg
     6541 agaagccggc ccccgacgcc gagaaggcgc agcaatgata agacttctac tttcagttct
     6601 gctgtatgcc gcggcgctcg cggccgcggc gcagactttc gtggtgcctt ccgagctgtg
     6661 ggaccgcccg cgcagcggcc gcgcggtgct cgagcaaccg gcgatccgcc aggcggtgaa
     6721 cgcctgcctg gcgcaaccgg gcgcgcgcct ggtggtccgc cacggcgccg ggcaggaatc
     6781 gcagctcgcc gcggaggaat tgcgctcctg gctcgcggcg ctggcggtcg agcccgggcg
     6841 catctcgctg cgcggcgatc tgccatcgtc ccagccgctc cagctcgaag tgattcgtga
     6901 ctgatagggc gtgactgata atgcgtgact ggtacgacgt gactggtgaa gcgtgactga
     6961 agatacgggt ttacttccga ctttcgactt ccgactttcg actttatgaa cgcggcgcgt
     7021 tcatccttcg gcagggccag gccgcacgtc gcgaaatccg gcgggcagcc cgcggtgacg
     7081 cgcatcgccg cggtgcagct cgcttccggt cccaatatca agggcaatct ccacgagacg
     7141 gggcggctcg tcgagatggc ggtggcgcag ggcgcgaagc tggtggcgct tcccgagtat
     7201 ttcgcgatca tgggcttgcg cgaccaggac aaggtcaagt tgcgggagaa gccgggcgcg
     7261 ggcccgatcc aggaattcct ctcgacaacg gc
//
LOCUS       DBNGWZ010000218         5081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2541426, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000218 DBNGWZ010000000
VERSION     DBNGWZ010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2541426"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="AC16CX_06515"
     CDS             complement(<1..743)
                     /locus_tag="AC16CX_06515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3012203.1"
                     /translation="MAVEEQATRAGMRASYDAIVVGGGHNGLVCAAYLARAGKRVLVL
                     ERRERIGGAALSEEVFPGFTFSVFSYVVSLLRPEIIRELELPRHGLHILPLESTLTPL
                     ANGDYLAQWSDPDQNRREIARHSARDAEAVEAFGRLMHQMARAVKPLLGMVPPDPASL
                     SPRDLFGLARLASHFRGLGADRFHALCKLLTMSAADYLDEWFETEALKATKSASGIIG
                     TLAGPRSPGTAYVLLHHYMGEIDGVFRAWG"
     gene            complement(763..2643)
                     /locus_tag="AC16CX_06520"
     CDS             complement(763..2643)
                     /locus_tag="AC16CX_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop nucleotide/nucleoside kinase family
                     protein"
                     /protein_id="HIR3012204.1"
                     /translation="MDEPLTATAVAGAGPAQYGAWKPGIQSELPWPLLPLSTIFRPEN
                     VFTTVDQAHELSQVTGLNADELVLFRPERLVVHELLVRVTADFSVPDGPRVEDLGIHF
                     REMTRTILTRYIEPHMREIIDTYHLLQRALVTLIGAELSAALSALSTDAGIAPAKEAG
                     TGGLLGLLRRRRRGDGAPRDEDRWQREERLSREWAAKAQLSGEPLARAAYRSLVRVAA
                     AVRGRHGRMWGDRELLASIAAGMACNEHGAEVIGGFIEPHLREAAAREGYRALPAQEL
                     PMVMNTKGAPASGKSTLRPLQRKLAARIGVHWSEFALISPDIWRKFLLDYGTLGEHYK
                     YAGTFTARELAIVDQKLDRYMARKAERGGMSHLLIDRFRFDSFAPDSDEAGSNLLTRF
                     GRLVYLFFMITPPHETVERAWKRGLEVGRYKAVDDVLAQNIEAYTGMPELFFTWALRP
                     GKRVHYEFLDNSVPLGEQPRTVAFGWNGEMNILDVKCMLDIDRYRKIDVDAADPRRVY
                     PDHETMAAENNTQFLARCARELPAVNFAQRESGRVYARIEGGKLAWTDPEILESATQD
                     SETRAGLLAVAPAAVRGTNHAGAGPKRQTAVLQAERFHTLGRWGRAARQGQESAAGPV
                     LP"
     gene            complement(2657..3844)
                     /locus_tag="AC16CX_06525"
     CDS             complement(2657..3844)
                     /locus_tag="AC16CX_06525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3012205.1"
                     /translation="MLKTTPFHARTAPLCVSHAWRRWAGYVVASSYELSHEREYHAIR
                     SSAALLDVSPLYKYLVRGKDAARLLDRVMTRAIARAQTGQVLYTPWCDATGKVLDDGT
                     IARLDEEIFRVTSADPNLRWLEDNAVGLHVAVDDVSESTAALALQGPASRAILQQLSD
                     TDLAGLKYYRLTAARLRGIPVTISRTGYTGDLGFEIWMDAAHALPLWDALIEAGTPCG
                     ITPAGMLALDVARIEAGLILMDVDYISARKALIGSQTSSPFELDLGWAVSLDKENFVG
                     RAALAEEKRRGPQWQFVGIEVEWDALERLYAEVDLAPRLPTAAWRASVPLYADGEQVG
                     YATSGGWSPLLKRYIALAHVRSRWATPATPVEMEVTVEHRRKRTSARVVRKPFFNPER
                     KRS"
     gene            complement(3844..>5081)
                     /locus_tag="AC16CX_06530"
     CDS             complement(3844..>5081)
                     /locus_tag="AC16CX_06530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3012206.1"
                     /translation="MLDRAGGVLRGMLLETPPNVGGGARAALAALRAGYRMRKAGMAA
                     RRDLFDLMTKSAGDLLDAWFESAPIKAAFGFDAVVGNYASPYAPGTAYVLLHHVIGEV
                     NGRRGAWGHAVGGMGAITQAMAKEAERRGVEITTGCEVYKVCVDDRGAQGVQLADGSM
                     VDARCVVAGLDPRLLFLKLVDERVLDADFLRRIRGYKCGSATFCINVALSELPDFTCL
                     PGKHLQAHHQSGIIIGPSLAYMEQAYFDARTHGCSRAPIVQMLIPSTVDSTLAPPGKH
                     VASLFCQHFAYDLPNGLDWDEIREPAADLVIDTVTRYAPNLRSAVIGRKVLTPLDLER
                     EFGLTGGDIFHGALTLDQIWSARPVLGHADYRAPVAGLYLCGAGAHPGGGVSGLPGHN
                     AAREILRDFRSGRLDHKHK"
BASE COUNT          778 a         1766 c         1731 g          806 t
ORIGIN      
        1 ccccaggcgc ggaacacgcc gtcgatctcg cccatgtaat ggtgcagcag cacgtaggcg
       61 gtccccggcg agcgcggccc ggcgagcgtg ccgatgatcc cgctcgcgga cttcgtcgcc
      121 ttcagcgcct cggtttcgaa ccattcgtcg agataatcgg ccgcgctcat cgtgaggagc
      181 ttgcaaagcg cgtggaagcg gtctgcgccc aggccgcgga aatgcgaggc gaggcgcgcc
      241 aggccgaaca ggtcgcgggg tgagagcgag gccgggtcgg gcggaaccat gccgagcagc
      301 ggctttaccg cgcgcgccat ctggtgcatg agacgtccga acgcctcaac agcctcggcg
      361 tcgcgcgccg agtggcgggc gatctcgcgg cggttctggt ccggatcgct ccactgcgcg
      421 aggtaatcgc cgttcgcgag cggcgtcagc gtgctctcga gcggcaggat gtgcaggccg
      481 tggcgcggca gctcgagctc gcggatgatc tcgggccgca gcaggctcac cacgtacgag
      541 aacaccgaga acgtgaagcc gggaaagacc tcctcgctca gcgccgcgcc gccgatccgc
      601 tcgcgccgct ccagcaccag gacccgcttg ccggcgcggg cgagatacgc ggcgcacacc
      661 agcccgttgt gcccgccgcc gaccacgatg gcgtcgtaag aagctctcat ccccgcgcgc
      721 gtggcctgct cttccacagc catcactccg agctgcgagc ggtcacggga gaaccgggcc
      781 cgcagcggat tcctgcccct gccgggccgc tctcccccac cgcccgagcg tgtgaaatcg
      841 ctctgcctgc aacaccgcgg tctgccgctt cgggcccgcg ccggcgtggt tcgtgccgcg
      901 cacggccgca ggcgcgacgg cgaggagccc ggcgcgggtc tcgctgtcct gagtggcgct
      961 ctccaggatc tcggggtcgg tccacgcgag ctttcccccc tcgatgcgtg cgtagacgcg
     1021 gccactctcg cgctgcgcaa agttcaccgc cggaagctcg cgcgcgcacc gcgccaggaa
     1081 ctgcgtgttg ttctccgccg ccatggtctc gtgatcggga tagacccgtc gcggatcggc
     1141 ggcatcgacg tcgatcttgc ggtagcgatc gatatcgagc atgcacttga cgtcgagtat
     1201 gttcatctcg ccgttccagc cgaaggcgac ggtccgcggc tgttctccga gcggcacgct
     1261 gttgtccaga aattcatagt gcacccgctt gccgggccgc agcgcccacg tgaagaacag
     1321 ctcgggcatg ccggtgtagg cctcgatgtt ctgtgcgagc acgtcgtcga ccgccttgta
     1381 gcggcccacc tcgagaccgc gcttccaggc gcgctcgacg gtctcgtgcg gcggcgtgat
     1441 catgaagaac aggtagacca ggcggccgaa gcgggtgagc agattgctgc ccgcctcgtc
     1501 cgagtccggc gcgaagctgt cgaaccggaa ccggtcgatc aggagatgcg acatgcctcc
     1561 gcgctcggcc ttgcgcgcca tgtagcggtc gagcttctgg tcgacgatcg cgagctcacg
     1621 ggcagtgaag gtcccggcat acttgtaatg ctctcccagc gtgccgtagt cgaggagaaa
     1681 cttccgccag atgtccggac tgatcaaggc gaactcgctc caatgcacgc caatgcgggc
     1741 tgcgagcttt ctctgcaggg ggcgcagcgt gctcttgccg gaggcgggcg cgcccttggt
     1801 gttcatcacc atcggcagct cctgcgcggg aagcgcgcgg tagccttccc gggccgcggc
     1861 ctcgcgcaga tgcggctcga tgaacccgcc gatcacctcg gctccgtgct cgttacaggc
     1921 catgccggcg gcgattgagg cgagcagctc ccggtccccc cacatccgcc cgtgccgccc
     1981 gcgaaccgcc gctgcgactc tcaccaatga acggtaggcg gccctcgcca gcggctcccc
     2041 gctcaactgc gctttcgcgg cccactcccg ggacagccgt tcctcgcgct gccagcgatc
     2101 ctcgtcgcgg ggcgcgccat ccccgcgccg ccggcggcgg agcaggccga gcaggccgcc
     2161 cgtcccggct tccttcgcgg gcgcgatacc ggcatccgtc gacagcgcgg agagcgccgc
     2221 cgagagttct gcgccgatca gcgttacgag cgctcgctgg agcaagtggt acgtatcgat
     2281 gatctcgcgc atgtggggtt cgatgtagcg ggtgaggatc gtccgtgtca tctcgcggaa
     2341 atggatgccg agatcctcga ccctgggacc gtcaggtacc gagaagtcgg cagtcactcg
     2401 aaccagcagt tcgtgaacca cgagccgctc cggccgaaac agcacgagct catcggcatt
     2461 gagtcctgtg acctggctga gctcatgggc ctgatcaacg gtcgtgaaga cgttctccgg
     2521 ccggaagatc gtggagaggg ggagcaatgg ccagggcagt tcggactgaa tgcccggttt
     2581 ccacgcccca tattgcgcgg gcccggcacc ggccaccgct gtcgcagtca agggctcgtc
     2641 catggtgtgt cgtgccctat gagcgcttgc gctcggggtt gaagaacggc ttcctgacca
     2701 cgcgcgcgct agtgcgtttg cggcggtgct ccacggtcac ctccatctcc accggcgtgg
     2761 cgggcgtggc ccagcgggag cgcacgtgag cgagcgcgat gtagcgcttg aggagcggcg
     2821 accagccgcc gctcgtcgcg tatccgacct gttcgccatc ggcgtagagg ggcacgctcg
     2881 cgcgccaggc ggcggtcggc agccgcgggg cgaggtcgac ttcggcgtag agccgttcga
     2941 gcgcgtccca ttcgacttcg attccgacga actgccattg cggcccgcgc cgtttctctt
     3001 cggcgagcgc cgctcttccc acgaagttct ccttgtcgag gctcacggcc cagccgagat
     3061 cgagctcgaa cggcgacgag gtctggctcc cgatgagcgc cttgcgcgcc gagatgtagt
     3121 ccacgtccat gaggatcagc cccgcctcga tccgcgccac gtcgagcgcg agcatccctg
     3181 caggcgtgat cccgcacggc gtgccggcct cgatcagcgc gtcccacagg ggaagcgcgt
     3241 gcgccgcatc catccagatc tcgaagccga gatcgccggt atagccggtg cgggagatcg
     3301 tgaccgggat cccgcgcagc cgcgccgcgg tcagccggta gtacttcagg ccggcgaggt
     3361 cggtgtcgct cagttgctgc aggatggcgc gcgaggcggg cccctggagc gcgagcgccg
     3421 cggtggactc ggatacgtcg tcgaccgcga cgtggaggcc cacggcgttg tcctccagcc
     3481 agcgcaggtt cgggtcggcc gaagtcacgc ggaagatctc ctcgtcgagc cgtgcgatgg
     3541 tgccgtcgtc gagcaccttt ccggtggcgt cgcaccaagg tgtatagagc acctggcccg
     3601 tttgcgcgcg cgcgatcgcg cgcgtcatca cgcgatcgag caggcgcgcg gcgtccttcc
     3661 cccgcaccag atacttgtac agcggcgaga cgtcgagcag cgcggccgag ctccggatcg
     3721 cgtgatattc ccgctcgtgc gaaagctcgt aggagctggc gacgacgtag ccggcccagc
     3781 gccgccaggc gtggctcacg cacagggggg cagtgcgcgc gtggaacggt gtggttttca
     3841 gcatcacttg tgcttgtggt cgagcctgcc cgaacggaaa tcacgcagga tctcgcgggc
     3901 cgcgttgtgc cccggcagcc ccgacacgcc gccgcccggg tgcgcgcccg ctccgcacag
     3961 gtagagaccg gcgaccggcg cgcggtaatc ggcgtgcccc agcaccgggc gcgcgctcca
     4021 gatctggtcg agcgtcagcg cgccgtgaaa gatatcgccg ccggtgagcc cgaactcgcg
     4081 ctcgaggtcg agcggggtca acaccttcct cccgatcacc gcgctcctga ggttgggcgc
     4141 gtagcgcgtg acggtgtcga tcaccaggtc ggcagccggc tctctgattt cgtcccaatc
     4201 gagcccgttg ggtaaatcgt aggcgaagtg ctgacagaac aggctcgcga catgcttccc
     4261 gggcggcgcc agcgtggagt cgaccgtgga cgggatcagc atctgcacga tcggcgcgcg
     4321 cgagcagcca tgggtgcgcg catcgaaata ggcctgctcc atgtaagcga gcgaagggcc
     4381 gatgatgatg cccgactggt ggtgcgcctg caggtgtttt ccgggcaggc aggtgaaatc
     4441 cggaagttcc gagagcgcga cgttgatgca gaaggtcgcc gagccgcatt tgtagccgcg
     4501 gatgcgccgc aggaagtcgg cgtcgagcac gcgctcgtcg acgagcttca ggaacagcag
     4561 cctcgggtcg aggccggcca ccacgcagcg cgcgtccacc atgctgccgt cggcgagctg
     4621 cacgccctgc gccccgcggt cgtccacgca caccttgtac acttcgcacc cagtggtgat
     4681 ctcgacgccc cgccgctccg cttccttcgc catcgcctgc gtgatcgctc ccatgccgcc
     4741 caccgcatgg ccccacgcgc cgcgccggcc gttcacctcc ccgatcacgt ggtgcagcag
     4801 cacgtaggcg gtgcccggcg cgtacgggct tgcgtagttg ccgaccaccg cgtcgaaacc
     4861 gaaggccgcc ttgatcggcg cggattcgaa ccacgcgtcg agcaggtcgc ccgcgctctt
     4921 ggtcatgaga tcgaacaagt cgcggcgcgc ggccatgccg gccttgcgca tgcggtagcc
     4981 ggcgcgcaac gccgccagcg ccgcgcgcgc gccgccaccg acgttgggcg gggtctcgag
     5041 cagcatcccg cgcagcacgc cgccggcgcg atcgagcatc g
//
LOCUS       DBNGWZ010000219         5034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2919947, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000219 DBNGWZ010000000
VERSION     DBNGWZ010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2919947"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="dnaJ"
                     /locus_tag="AC16CX_06535"
     CDS             complement(<1..1103)
                     /gene="dnaJ"
                     /locus_tag="AC16CX_06535"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3012207.1"
                     /translation="MAKRDYYDVLGVNRDASEEDVKKAYRKLAMKWHPDRNPDNPKAE
                     EHFKEAKEAYEILSDGQKRAAYDQFGHAGVDPSAAAGAGFGAGAAGFGGFADAFGDIF
                     SDIFGGGRGRSSVYRGADLRYNLEISLEDAARGTETRIRIPAMEECATCHGSGAKPGT
                     SPTTCPACQGHGQVRMQQGFFSIQQTCPRCHGSGKIVTSPCPACGGAGRVKQHKTLSV
                     KIPAGVDEGDRIRLSGEGEAGVNGGPPGDLYVVIHLKPHPVFQRDHNDLHCEMPISFT
                     TAALGGDIEIPTLDGYAKIKVPTGTQTGQMFRLRGKGIKGVRTSSHGDLICHVAVETP
                     VNLTPRQKELLLELEAINQKDGGRHNPRAKSWM"
     gene            complement(1209..3140)
                     /gene="dnaK"
                     /locus_tag="AC16CX_06540"
     CDS             complement(1209..3140)
                     /gene="dnaK"
                     /locus_tag="AC16CX_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3012208.1"
                     /translation="MAKIIGIDLGTTNSCVAVMENGQPKVIENSEGARTTPSIVAYME
                     DGEILTGAPAKRQAVTNPRNTLYAVKRLIGRRFADSEVQKAIKVVPYQITEAANKDAW
                     VEVRGKKMAPPEISAQVLIKMKKTAEDYLGEPVSEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKQEKKDRKIAVYDLGGGTFDISIIEIANVEGEHQFE
                     VLSTNGDTFLGGEDFDQRLIEYLCDEFKKENGIDLRKDMLALQRLKDAAEKAKIELSS
                     SAQTEINLPYITADQAGPKHLAIKVTRAKFESLVEDLIEKTIEPCRVALKDAGVKAGD
                     VSDVILVGGQTRMPKVQEKVKAFFGREPRKDVNPDEAVAVGAAIQAGVLKGDVKDVLL
                     LDVTPLSLGIETLGGVMTKLIQKNTTIPTKAQQIFSTAEDNQTAVTIHVLQGEREMAR
                     GNKSLGQFNLADIPTAPRGMPQIEVTFDIDANGILHVTARDKATGKENKIRIQAGSGL
                     TEAEIQRMVKDAEEHAEEDRKAHELVEARNQCDHLVHSVRKTLKEHGGELSDDEKSKI
                     ETALKEAEESLKSDDKAAIEAKSQALAQAAHKLTEKMYAKEQAKAQAGGEAKTADDAK
                     KDEGNVVDAEYTEVKDKKG"
     gene            complement(3199..3825)
                     /gene="grpE"
                     /locus_tag="AC16CX_06545"
     CDS             complement(3199..3825)
                     /gene="grpE"
                     /locus_tag="AC16CX_06545"
                     /inference="COORDINATES: protein motif:HMM:NF010737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3012209.1"
                     /translation="MFKKPSAEKRMQETPEATNAETSPPAGELGAPRSPANQNAQSSV
                     GEEPLRETVPSAEELLKKAELAAAEHHDAWLRARAEADNIRKRAQVEIANAHKFAVEG
                     FAAELLAVKDSLEAALASEIASVDSMKSGVELTLKQLAGVFERFNLSEINPAGQKFDP
                     HRHQAISTVESDAEPNTVVQVLQKGYLLHERVIRPALVTVAKPNAVEK"
     gene            complement(3991..5034)
                     /gene="hemH"
                     /locus_tag="AC16CX_06550"
     CDS             complement(3991..5034)
                     /gene="hemH"
                     /locus_tag="AC16CX_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3012210.1"
                     /translation="MGVLLIQLGTPEAPTAAAVRAYLKDFLSDPRVIEIPRIIWWPIL
                     HLFVLTTRPKVSAQRYAQVWMNEGAPLRVHTERQLTLLRGYVGERAQQLPLTVDYAMR
                     YGRPSIPEKLWELKSQRCDRILLVPLYPQYSASATATAFDAAFSCLQATRNQPAIRTI
                     TGFHDHPGYIAALAQNVRDHWMKNGRSEALLMSFHGVPRATLTKGDPYHCECQKTARL
                     LAEALGLKQEQYGVSFQSRFGRAEWLRPYTADRLAELGKQKARRVDVICPGFVSDCLE
                     TLEEIAIEGKSIFLNAGGREFHYIPCLNERNDWIHALTDIVVGNLVGWTDTATHETLE
                     MSRLRALSMGAKS"
BASE COUNT          704 a         1637 c         1618 g         1075 t
ORIGIN      
        1 tccatccagg acttcgcccg cggattgtgg cggccgccgt ccttctggtt gatcgcctcg
       61 agctccagca ggagttcctt ctgtcgcggc gtcaggttga ccggcgtctc gaccgcgacg
      121 tggcagatga ggtcgccatg gctgctcgtg cgcacgccct tgatgccctt gccgcggagg
      181 cgaaacatct ggcccgtctg cgtgccggtc ggcaccttga tcttggcgta gccgtcaagc
      241 gtcgggatct cgatgtcgcc gccgagggcc gcggtggtga agctgatcgg catctcgcag
      301 tggagatcgt tgtgatcgcg ctgaaacacc gggtggggtt tgagatggat caccacgtac
      361 agatcgcccg gcgggccgcc gttcacgccg gcttcgccct cgcctgacag ccggatgcgg
      421 tcgccctcgt ccacgccggc ggggatcttc accgacagcg tcttgtgctg cttgacgcgc
      481 ccggcgccgc cgcaggccgg gcacggcgaa gtcacgatct tgccgctgcc gtggcagcgc
      541 gggcaggtct gctggatcga gaagaagccc tgctgcatcc tgacctgccc gtgtccctga
      601 caggcggggc aggtggtggg ggaggtgcca ggcttggcgc cgctgccatg gcaagtggcg
      661 cattcctcca tcgccgggat gcgaatgcgc gtttcggtgc cgcgcgcggc gtcctcgagc
      721 gagatctcga ggttgtagcg caggtccgcg ccgcggtaca cgctcgagcg gccgcgcccg
      781 ccgccgaaga tgtcgctgaa gatgtcgccg aaggcgtcgg cgaagccgcc gaacccggcg
      841 gcgccggcgc cgaagcccgc gccggccgcc gccgacggat ccacgcccgc gtgcccgaac
      901 tgatcgtaag ccgcgcgctt ctggccgtcc gacaggatct cgtaggcttc cttggcttcc
      961 ttgaagtgct cttccgcctt ggggttatcc ggattgcggt ccggatgcca cttcatggcg
     1021 agcttgcggt acgccttctt gacgtcctct tccgaagcgt cccggttgac gccgagcacg
     1081 tcgtagtagt cgcgcttcgc catgtcagtt gctgttcacc ttccaatacg aaacggccga
     1141 gttgaacggc ggcctgcgcc gtccgttcga actcggccag ctaaaccggc gcgcttcctg
     1201 cgccggattt agcctttctt gtctttcacc tcggtgtact ccgcgtccac gacgttgcct
     1261 tcgtccttct tggcatcgtc ggcggttttc gcctcgccgc cggcctgcgc tttggcctgc
     1321 tccttggcgt acatcttttc cgtgagcttg tgcgcggcct gggccagggc ctggctcttg
     1381 gcctcgatcg ccgccttgtc atcgctcttg agcgattcct cggcttcctt gagcgccgtc
     1441 tcgatcttgg acttctcgtc gtcggaaagc tcgccgccgt gctccttgag cgttttcctg
     1501 accgagtgca ccaggtggtc gcactggttg cgcgcctcca ccagctcgtg cgccttgcgg
     1561 tcctcctcgg cgtgctcctc ggcgtccttc accatgcgct ggatctcggc ttcggtgaga
     1621 cccgagccgg cctggatccg gatcttgttt tccttgccgg tcgccttgtc cctggcggtc
     1681 acgtgcagga tgccgttcgc gtcgatgtcg aacgtgactt cgatctgcgg catgccgcgc
     1741 ggcgccgtcg ggatatcggc caggttgaac tggccgaggc tcttgttgcc gcgcgccatc
     1801 tcgcgctcgc cctgcagcac gtggatcgtg accgcggtct ggttgtcttc cgcggtcgag
     1861 aagatctgct gcgccttggt cggaatggtc gtgttcttct ggatgagctt ggtcatcacg
     1921 ccgccgagcg tctcgatgcc gagcgagagg ggcgtgacat cgagcagcag cacgtccttg
     1981 acgtcgccct tcagcacgcc ggcctggatt gcggcgccga ccgccaccgc ctcgtccggg
     2041 ttgacgtcct tccgcggctc gcgcccgaag aacgccttca ccttctcctg caccttgggc
     2101 atgcgcgtct ggccgcccac caggatcacg tcggagacgt cgcccgcctt gacgccggcg
     2161 tccttgagcg caacgcggca cggctcgatg gtcttctcga tcaggtcctc gaccaggctc
     2221 tcgaacttgg cgcgcgtcac cttgatcgcg aggtgcttgg gcccggcctg gtcggccgtg
     2281 atgtagggca ggttgatctc ggtctgcgcg ctcgaggaga gctcgatctt cgccttttcg
     2341 gcggcgtcct tgagccgctg cagcgcgagc atgtccttcc ggagatcaat gccgttctcc
     2401 ttcttgaact cgtcgcacag gtactcgatc aggcgctggt cgaagtcctc gccgccgagg
     2461 aaggtgtcgc cgttggtgga gagcacctcg aactggtgct cgccctcgac gttggcgatc
     2521 tcgatgatcg agatgtcgaa ggtgccgccg ccgaggtcat agaccgcgat cttgcggtct
     2581 ttcttctcct gcttgtcgag gccgaacgcg agcgcagccg cggtcggctc gttgatgatg
     2641 cgcttcacgt cgaggcccgc gatgcggccg gcgtccttgg tcgcctggcg ctgggaatcg
     2701 ttgaagtagg cgggcaccgt gatcaccgcc tcggagaccg gctcgccgag gtagtcctcc
     2761 gcggtctttt tcatcttgat cagcacctgc gccgagattt ccggcggcgc catcttcttg
     2821 ccgcgcacct cgacccaggc gtccttgttg gcggcttccg tgatctggta cggcaccacc
     2881 ttgatcgcct tctgcacttc gctgtcggcg aaacggcggc cgatcagccg cttcaccgcg
     2941 tagagcgtgt tcctgggatt ggtgacggcc tggcgcttgg ccggcgcgcc ggtgaggatt
     3001 tccccgtctt ccatgtaggc gacgatggag ggggtggtgc gggcgccctc ggagttttcg
     3061 atcaccttgg gctggccgtt ttccatgacg gccacgcacg agttggtcgt tcccaggtcg
     3121 atgccgatga tcttggccat gtgctacctc tttggatttt caacaatatt ggtcatatcc
     3181 ggatatgggg cccgtccctc atttttcaac cgcattcggc ttggcaacgg tgaccagcgc
     3241 ggggcgaatc acccgttcgt gcaagaggta tcccttttgc agcacctgga ccaccgtatt
     3301 gggttcggcg tcggactcca ccgtgctgat cgcctggtgg cgatggggat cgaatttctg
     3361 acctgccgga ttgatctcgg agaggttgaa gcgctcgaac acgcccgcaa gctgcttcaa
     3421 cgtcaactcg acgccgctct tcatgctgtc caccgacgcg atttccgagg cgagcgccgc
     3481 ctcgagactg tccttcaccg ccagcagttc ggccgcgaag ccctccacgg cgaacttgtg
     3541 cgcgttggcg atctcgactt gcgcgcgctt gcggatgttg tcggcttccg cccgggcgcg
     3601 cagccatgcg tcgtgatgct ccgcggccgc aagctcggct ttcttcaaca gctcctccgc
     3661 actgggcacg gtctcgcgaa gcggttcttc accgaccgat gactgggcgt tctgattagc
     3721 tggagaccgg ggagcaccga gctccccggc gggcgggctg gtttcagcgt tggtagcttc
     3781 gggcgtttcc tgcatcctct tctccgcgct tggctttttg aacattgggc cggaccaagg
     3841 taattcaagc gctatggtat ctatctaaca tactgattga atgcagcttt tcttactgct
     3901 cacgaataag gcagaaggcg gaaggatgaa ggatgatcca acagcgggtt tcgcttcaaa
     3961 cttctgcctt cttccttccg ccttcatcct tcaggatttc gctcccatgg acaacgcgcg
     4021 caaccgcgac atttcaagcg tctcatgcgt cgcggtatcg gtccagccca cgagattgcc
     4081 cacgacgatg tcggtcagcg cgtgtatcca gtcgttgcgc tcgttgaggc agggaatgta
     4141 gtggaactcg cgaccgcccg cattgaggaa aatgctcttc ccttcgatcg cgatctcctc
     4201 gagggtttcc aggcagtcgc tcacgaagcc ggggcagatc acgtcaacgc gcctcgcctt
     4261 ctgcttgccg agctcggcga gcctgtcggc ggtgtagggc ctgagccatt ccgcgcgccc
     4321 gaagcgcgac tggaacgata cgccgtactg ctcctgtttg agcccgaggg cctccgcgag
     4381 caaccgcgcg gttttctggc actcgcaatg gtaggggtcg cccttggtga gcgtcgcgcg
     4441 cggcacgccg tggaagctca tgagcagcgc ctcggagcgg ccgttcttca tccagtggtc
     4501 gcgcacgttc tgggcgagcg cggcgatgta gcccggatgg tcgtgaaagc cggtgatcgt
     4561 gcggatcgca ggctggttgc gcgtggcctg cagacagctg aacgccgcgt cgaaggcggt
     4621 cgccgtggcg ctggccgagt actggggata gagcgggacc agcaggatgc gatcgcagcg
     4681 ttgcgacttc agctcccaga gcttctccgg gatcgagggt cttccgtagc gcatcgcgta
     4741 atccacggtc agcggcagct gctgcgcccg ctcgccgaca tagccgcgca gcaatgtcag
     4801 ctggcgctcg gtgtgcaccc gcagcggggc gccctcgttc atccacacct gggcatagcg
     4861 ctgggccgaa accttgggcc gcgtggtgag cacgaaaaga tgcaggatcg gccaccagat
     4921 gatgcgcggg atctcgatca cccgcgggtc ggacaggaag tccttgagat aggcgcgcac
     4981 cgccgccgcc gtcggcgctt ccggcgtgcc gagctggatc aggaggaccc cgat
//
LOCUS       DBNGWZ010000220         1919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1155274, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000220 DBNGWZ010000000
VERSION     DBNGWZ010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1155274"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16CX_06555"
     CDS             <1..350
                     /locus_tag="AC16CX_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012211.1"
                     /translation="LKICSAHGGGYLPSYAARSDNSLRVAPDMDTGVKLKKKPTEYLR
                     TMYYDTLVFTSEALRHLAAEVGSSQLVIGTDHPIPWQSKSVDHILNTPGFTDKERCMM
                     LGETAAKLLGIKP"
     gene            514..1497
                     /locus_tag="AC16CX_06560"
     CDS             514..1497
                     /locus_tag="AC16CX_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012212.1"
                     /translation="MRLFRAASLALTAGLALYPAVSPAQESGPAPGFPRQMRIIIASV
                     PATGPDFIARLIAPKLGAAWRVNVVVENRPGSNGIVGAQFVAKSPPDGSVIMMGNAGT
                     HAINAALYKKLEYDPLQDFAAISEIASIPLALVVHPSVPAKSVRELIALARKSPGKLN
                     VAIAGAAGELMGNALKLQAKIDMKNIPYKGGSQAALAVLSGEADMVFTSYVVIAPHVE
                     AGKLRVLGVSSARRMPQLPGVPTVAENGLAGYEHEQWYALFAPAKTQAHLVQAMYREV
                     ARVVGLPEINERLVATGHRVIAGTPEQLSDKVRREIEKTRRIMQASGMQQQ"
     gene            complement(1660..>1919)
                     /locus_tag="AC16CX_06565"
     CDS             complement(1660..>1919)
                     /locus_tag="AC16CX_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR3012213.1"
                     /translation="AVLFLRNQALRLSELGALDSRAEANANDGCRASCVDWYGNSRPI
                     FVGNRVFALMGYEIVEGEIAANRLAEVRRVSFSPVTVSWAR"
BASE COUNT          374 a          637 c          618 g          290 t
ORIGIN      
        1 ggctgaagat ctgctcggcc cacggcggcg ggtacctgcc ctcgtacgcc gcgcgctccg
       61 acaacagcct gcgcgtcgcc ccggacatgg acaccggcgt caagctgaag aagaagccga
      121 ccgagtacct gcggacgatg tactacgaca cgctcgtgtt cacgtccgag gcgctgcggc
      181 acctggcggc ggaagtcggc tcgagccagc tcgtcatcgg caccgaccat ccgatcccgt
      241 ggcagagcaa gtcggtggac cacatcctca acacgccggg cttcaccgac aaggagcgct
      301 gcatgatgct cggggagacc gcggcgaagc tgctgggaat caagccttag cccggccggc
      361 gaccgggcgg tgataagtga taggtgatag gagccgagac aaaaagcgtg ataggtgata
      421 ggagtaacta atctcccctg cgcgggagtc ttatcactta tcactcgtca cttatcacgc
      481 cgttgcgcgg gagcggatga ggaagggggt ctcatgaggc tgtttcgtgc agcatcgctt
      541 gccttgacag cgggtctcgc gctgtatccc gccgtctcgc ccgcgcagga atccgggcct
      601 gcgccgggct tccccaggca gatgcgcatc atcatcgcga gcgtgcccgc caccggcccg
      661 gacttcatcg cccgcctgat cgcgcccaag ctcggcgcag cgtggcgggt caacgtggtg
      721 gtggaaaatc ggccgggatc gaacggcatc gtcggcgcgc aattcgtcgc caaatccccg
      781 cccgacggct cggtcatcat gatgggcaac gcgggcaccc acgcgatcaa cgccgccctc
      841 tacaaaaagc ttgaatacga cccgctacag gatttcgccg ccatcagcga gatcgcttcg
      901 atcccgctgg cgctggtcgt tcacccgtcg gttccggcga agtccgtccg ggagctgatc
      961 gcgctcgcca ggaaatcgcc cggcaagctc aacgtcgcca tcgcgggagc ggcgggcgag
     1021 ctcatgggca acgcgctcaa gctgcaggcg aagatcgaca tgaaaaacat cccctacaag
     1081 ggaggctcgc aggccgcgct cgccgtgctg tccggcgaag cggacatggt tttcacgtcg
     1141 tatgtcgtca tcgcgccgca cgtcgaagcc ggaaagctga gggtgctcgg cgtcagcagc
     1201 gcccggcgca tgccgcagct tcccggcgtg ccgacggtcg ccgagaatgg cctcgccggc
     1261 tacgagcacg agcaatggta tgcgctgttc gcgccggcga agacgcaggc ccatctggtg
     1321 caggcgatgt accgggaagt cgcgcgcgtc gtgggcctcc cggagatcaa cgagcgcctc
     1381 gtcgccaccg ggcatcgggt catcgcaggc accccggagc agctctccga caaggtccgg
     1441 cgcgaaatcg agaagacccg cagaatcatg caggcgtcag gaatgcagca gcagtgaaga
     1501 agcaaggatg aaggcggaag gatgaaggat gagggacaag atttccgtga gagcgtgaaa
     1561 gcgtcagggc gggaggggaa accactccat ctcacgatct cccgctctaa cgattccacg
     1621 tcgcatcacc catcactcat cactcatcac cggcccgcgt cagcgggccc aactgaccgt
     1681 caccggcgag aagctcacgc gccgcacttc cgccagccgg ttggcggcga tctcgccctc
     1741 gacgatctcg tagcccatca gcgcgaacac gcgattcccg acgaagatcg gccgggagtt
     1801 gccgtaccag tccacgcacg aagcgcggca gccgtcgttc gcgttggcct cggcgcgcga
     1861 gtcgagcgcg cccagctcgc tcagccgcag cgcctggttg cgcaggaaca gcaccgcgg
//
LOCUS       DBNGWZ010000221         4667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1910832, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000221 DBNGWZ010000000
VERSION     DBNGWZ010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1910832"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="AC16CX_06570"
     CDS             <1..569
                     /locus_tag="AC16CX_06570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3012214.1"
                     /translation="VGHRQTRNRGTLGGSLCQLDPSAELVAVAAALDATVSVTGPKGG
                     RELPFAEFPAAYMTPALEAGEMVTGARFPVWKKGHGHAFVEFARRHGDFAVVSAAVLL
                     EEDRAGKIVRASVTIGGTGPAPVRVKEAERALVGNVASAKLFREAGECCRKLDAMGDV
                     HAPAPYRRQLAAVLSRRALEKAHSRVSG"
     gene            575..1066
                     /locus_tag="AC16CX_06575"
     CDS             575..1066
                     /locus_tag="AC16CX_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012215.1"
                     /translation="MAEMRKIAVTVNGRQYQETVETRHTLADFLRQQLGLTGTHLGCE
                     HGVCGACTVLLDGRSVRSCLMLAVQANGHEITTVEGIAPGKTELHPLQEAFRDNHGLQ
                     CGFCTPGILTTLIEFLRDNPDPTEQEVRIAISGNLCRCTGYQGIVAAALDAAKRLQRS
                     RRG"
     gene            1059..3407
                     /locus_tag="AC16CX_06580"
     CDS             1059..3407
                     /locus_tag="AC16CX_06580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012216.1"
                     /translation="MASVSGKVQGSSVGRLEDTALLRGEGRFVDDIRLPGMLHAAFVR
                     SPHAHATIRGIGKTAALALHGVRAVLTLADLRPYLRNERIVVALPSPSYKQDRNRPAL
                     AGDETVHVGEPVAIVIADDRYIAEDAAALVAVDYDPLPAAADCRAALAPDAPKVHRDA
                     QHNLLAEFSMGYGDVDRAFAGAAHVFKESLWQHRGGSHSIECRGAVAVHDAIEDKLTL
                     WCSTQMPHAALRVLVDMLGRDENRLRVVTPDIGGGFGPKLVVYPEDVVTAIAALITGR
                     PVKWIEDRREHFIATTQERDQYWEVEIAVDRDARILGVRGSIVHDHGAYTARGVNLAH
                     NSAETVTLPYEVPAYRMNVRLALTNKVPVTPVRGAGHPQGTFAMERLLDRVARELKLD
                     RTDVRRRNLIPAAKMPYTKPLKTRGGMQVVLDSGDYPKCQQEACARAGWAAFRARQET
                     ARKEGRYIGIGLANFVKGTGRGPFEGATVRIGPSGRVHVYSGAAAMGQGTRTMLAQIV
                     AEQLGGDMANITVTIGDTAAIPIGIGTSNSRSTVTAGSSAHLAAKKVRQKALRVAALL
                     LKASEQDLEIVGGEVRVKGAPDMKVGLGRIAHTVAGTPGYALPGEVEPGMETTEYFVL
                     DDMAYANGTAVAEVEVDIETGAVRILRYVVAHDCGRILHPVIVDGQILGGAAHGIGNA
                     LYEWMGYDESAQPVTTNLGEYLLITATEMPPVEIIHQESPSPLNPLGVKGVGECGVVP
                     APAAIISAIEDALSPFGVHITRTPLFPAEIVVLINKGVMGDK"
     gene            complement(3468..4388)
                     /locus_tag="AC16CX_06585"
     CDS             complement(3468..4388)
                     /locus_tag="AC16CX_06585"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3012217.1"
                     /translation="MNPIVLKFGGYQEPASIHNRAAARFGELLGQRLGDRVRFELIGS
                     ILKLGRGSGDLVPMVERGELSFCYISTVRFSRAAPALQLLELPFVVRERRTAFNALDG
                     EYGKQAARLIDENTSCRLLGYWDNGFRHLTNRVRPIRRPEDCRGIRIRTQMSDLHGEV
                     FRALGFEPIKCDVKEFVEQIGTDRFQAQDNPLTNIYNFGIHNHHRYITLSRHFWGASA
                     MVCNAAHYRGWPKDVQAAVDASAREAMAYQRQLAAAEDDEIMAKLDPAKNEVITLTEP
                     ERGAFIKAVEPVLAKHRKQLDPKLFAYLEA"
BASE COUNT          816 a         1493 c         1576 g          782 t
ORIGIN      
        1 aggtcggcca ccgccagacg cgcaatcgcg gcacgctcgg cggctcgctg tgccagctcg
       61 atccctcggc ggagctggtc gcggtcgccg ccgcgctcga cgcgacggtc agcgtcaccg
      121 gcccgaaggg gggccgtgaa ctgccgttcg ccgaattccc cgcggcctac atgacgcccg
      181 cgctcgaagc cggtgaaatg gtgaccggcg cgcgcttccc ggtgtggaag aaggggcacg
      241 ggcacgcttt cgtcgaattc gcgcggcgcc acggggattt tgcggtagtg tccgcggcgg
      301 tgctgctcga ggaggatcgc gccgggaaaa tcgtacgcgc gtcggtcacc atcggcggca
      361 ccggtcccgc cccggtgcgg gtcaaggaag ccgaacgggc gctggtcggc aacgtcgcat
      421 ccgccaaatt gttccgcgaa gcgggtgaat gctgccgcaa gctcgacgcg atgggcgacg
      481 tgcacgctcc ggcgccgtac cggcgtcaac tggcggcggt gctgtcgagg cgcgcgctgg
      541 aaaaggcgca ttcgagagtt tccgggtgag tgtgatggcg gaaatgcgga aaatcgcggt
      601 cacagtgaac ggccgccagt accaggagac ggtggagacc cgccatacat tggcggattt
      661 tttgcgccag cagctcgggc tcaccggcac gcatctcggc tgcgaacacg gagtgtgcgg
      721 cgcctgcacc gtcctgctcg acgggcgttc cgtgcgctca tgcctgatgc tcgccgtgca
      781 ggcgaacggc cacgagatca cgacggtcga aggcattgcc ccggggaaaa ccgagttgca
      841 cccgttgcag gaggcgttcc gcgacaacca tggcctgcaa tgcggctttt gcacgccggg
      901 catcctgacg acattgatcg agttcctgcg cgacaacccg gacccgacgg aacaggaggt
      961 gcggatcgcg atctcgggca acctctgccg ctgcacggga tatcagggca tcgtcgccgc
     1021 cgcgctcgac gccgcaaagc gcttgcaaag gagtcgacgt ggctagcgtg agcggaaaag
     1081 tgcaaggcag cagcgtcggc cggctcgagg ataccgcgct cctacggggg gagggtcgct
     1141 tcgttgacga catccgcctg cccggcatgc tgcatgccgc gttcgtgcgc agcccgcatg
     1201 cccatgccac gatacgcggc atcggcaaga cggcagcgct tgctctgcat ggagtacgtg
     1261 cggtgctcac gctcgcggac ctgcgtccct atttgcgcaa cgagcggatt gtcgtcgctc
     1321 ttcccagccc aagttacaaa caggatcgca accggcccgc gctggccggc gacgaaacgg
     1381 tccacgtcgg cgagccggtt gcgatcgtga tcgccgacga ccggtatatc gccgaggatg
     1441 ccgccgcgct cgtcgcggtg gattacgatc ccctgccggc ggccgctgac tgccgcgctg
     1501 cgctcgcgcc tgacgcaccg aaggtccacc gcgacgcgca acacaacctg ctcgccgaat
     1561 tcagcatggg ctacggtgac gtggaccgcg ccttcgccgg cgcggcccat gttttcaagg
     1621 aatcgctgtg gcagcaccgc ggcggcagcc attccatcga atgccgcggc gccgtcgcgg
     1681 tccacgacgc gatcgaggac aagctgacgc tgtggtgctc cacgcagatg ccccacgccg
     1741 cgttgcgcgt gctggtcgac atgctcgggc gcgacgagaa ccggttgcgg gtggtgacgc
     1801 ccgacatcgg cggcggattc gggcccaagc tcgtggtcta cccggaggac gtcgtcaccg
     1861 ccatcgcagc gctcatcacg ggccgtcccg tcaagtggat cgaggaccgc cgcgagcatt
     1921 tcatcgccac cacccaggag cgcgaccagt actgggaggt ggagatcgct gtggatcgtg
     1981 acgcgcgcat cctcggcgtg cgcggctcga tcgtccacga tcacggcgct tataccgcac
     2041 gcggcgtgaa cctggctcac aactcggcgg agacggtcac gctgccgtac gaagtgccgg
     2101 cgtaccggat gaacgtcagg ctcgcgctga ccaacaaggt gccggtgacg ccggtgcgag
     2161 gggcgggcca tccacagggc acgttcgcca tggagcggct gctcgaccgt gtcgcgcgcg
     2221 agctgaagct ggaccgcacc gacgtgcgcc gccgcaacct cattcccgcg gccaagatgc
     2281 cctacaccaa gccgctcaag acgcgcggcg gcatgcaggt ggtgctcgac agcggcgatt
     2341 acccgaaatg ccagcaggag gcatgcgcac gggcgggttg ggcggcgttt cgcgcacgcc
     2401 aggagacggc gcgcaaggag ggacgctaca tcggcatcgg gctggcgaat ttcgtcaagg
     2461 gcaccggccg cgggccgttc gaaggcgcta ccgtgcgcat cgggccgtcg ggcagggtgc
     2521 acgtctattc cggcgcggcc gcgatggggc agggcacccg caccatgctg gcgcagatcg
     2581 tcgccgagca gttgggcggc gacatggcga acatcacggt gacgatcggt gacaccgccg
     2641 cgatcccgat cgggatcggc acctcgaaca gccgctcgac ggtcaccgcg gggtcatcag
     2701 cgcaccttgc cgcgaagaaa gtgcgccaga aggcgctcag ggtcgctgcg ctcctgctca
     2761 aggcttcgga gcaggacctc gaaatcgtgg gcggcgaggt ccgcgtgaag ggggcgcccg
     2821 acatgaaagt cgggctcggc cgcatcgccc acaccgttgc cggtacgccg ggatacgcgc
     2881 ttccgggcga ggtcgagccc ggcatggaga ctaccgagta tttcgtcctc gacgacatgg
     2941 cctatgccaa cggcaccgcg gtggcggaag tggaggtgga catcgagacc ggcgcggtgc
     3001 gcatcctgcg ctacgtcgtc gcgcacgatt gcggacgcat cctccacccg gtgatcgtgg
     3061 acggacagat cctgggcggc gccgcgcacg gtatcggcaa cgccctctac gaatggatgg
     3121 gctatgacga gagtgcccag ccggtcacga ccaacctcgg ggaatatctg ctgatcaccg
     3181 cgaccgagat gccgcccgtc gagatcattc accaggagtc gccctcgccg ctgaatccgc
     3241 tcggggtgaa gggagtgggt gagtgcgggg tggtgccggc gcccgcggcg atcatctccg
     3301 ccatcgagga tgcgctgtcg ccgttcggcg tgcacattac caggacgccg ctatttccgg
     3361 cggagatcgt cgttttgatt aataaaggcg tgatgggtga taagtgataa gtgacgagaa
     3421 agtgcgtgaa acgccgacct tcacaaactc ctatcaccta tcacttgtca cgcttctagg
     3481 tacgcaaaca gcttgggatc gagctgtttg cgatgcttag ccagaacggg ctcgacggcc
     3541 ttgatgaacg cgccgcgctc gggttcggtt agcgtgatca cctcgttttt cgcgggatcg
     3601 agcttcgcca tgatctcgtc gtcctccgcc gccgcgagct gtcgctggta cgccatggct
     3661 tcacgggccg acgcgtccac ggcggcctgc acgtccttcg gccagccgcg gtagtgcgcc
     3721 gcattgcaca ccatcgcgct cgcgccccag aaatgcctgc tcaacgtgat gtagcggtgg
     3781 tggttgtgga tgccgaagtt gtagatgttg gtgaggggat tgtcctgggc ctggaaacgg
     3841 tcggtgccga tctgttccac gaactccttg acgtcgcact tgatcggctc gaagccgagc
     3901 gcgcggaaca cttccccgtg cagatcgctc atctgggtgc ggatgcggat gccgcggcag
     3961 tcttcgggcc tgcggatcgg ccgcacccgg ttcgtaagat gacggaagcc gttgtcccag
     4021 tagccgagca gccggcagga tgtgttttca tcgatcaggc gcgcggcctg tttcccgtat
     4081 tccccatcca gagcgttgaa tgcggtgcgc cgctccctga caacgaaggg cagctccagc
     4141 aactgcaacg ccggcgccgc cctggagaag cgcaccgtcg agatataaca gaacgacaac
     4201 tctccgcgct cgaccatcgg cacgaggtcg cccgatccgc ggccaagctt gaggatgctg
     4261 ccgatcagct cgaagcggac gcgatcgccg agtctctgcc cgagcagctc gccgaagcgc
     4321 gcggcggcgc ggttgtggat cgaggcgggc tcttggtatc caccaaactt cagaacaatc
     4381 gggttcattc agaggtcttg gtgggcaaga cgtggtcggt aactccaggg agcaaaagcg
     4441 tgacgagtga taagtgacag gttgcccgcg ctcgtgcgga agttggctcc tatcacttat
     4501 cacccatcac ctatcacgct tttaatgttt caattctttc ggcgctcccg gctgcttgag
     4561 cgggtgcgac ttgtcgaagg cttcgagctt catgcactcg ttgaataccc gcatcacggt
     4621 cgggaccggc gcggtgtcgc aattgaagaa acgggcgccg aatacct
//
LOCUS       DBNGWZ010000222         7151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2290089, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000222 DBNGWZ010000000
VERSION     DBNGWZ010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2290089"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="AC16CX_06590"
     CDS             complement(<1..712)
                     /locus_tag="AC16CX_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR3012218.1"
                     /translation="MKHELELGTLESGRNFTLPLDFVTRTLAIIGIRGWGKTVTATVI
                     AEEMCECGIPWIAFDPTSVWWGLRCNPDGTPGGYPVLVVGGPHGDLPLEKDEGPRIAE
                     AVLQENLCCVIDLKLESKNTWRKFVADFCDRLMELTPAVPHHIFLEEAPEFVPQRPMG
                     EQKRSLAAVDRLSRLGRNNGYGATLISQRYATVQKDVLTMCENLIAGLTIGKTDRDAM
                     KGWIEEVVQVTESGKKADE"
     gene            complement(709..972)
                     /locus_tag="AC16CX_06595"
     CDS             complement(709..972)
                     /locus_tag="AC16CX_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012219.1"
                     /translation="MSLTPSLERKAVEALASAQCACGGFKEPKHAFCGRCYAALPLEV
                     KRKLWRTFSEGFAEAYDEAVDWLRIHTERLTQGGLRFVDPEIQ"
     gene            complement(969..1157)
                     /locus_tag="AC16CX_06600"
     CDS             complement(969..1157)
                     /locus_tag="AC16CX_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012220.1"
                     /translation="MTADACWCCGAPLDLEGECPEGCCQDGCSECSDDLLCCTCQEVA
                     AGEIEGEEDELVEEDEDA"
     gene            complement(1154..1642)
                     /locus_tag="AC16CX_06605"
     CDS             complement(1154..1642)
                     /locus_tag="AC16CX_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012221.1"
                     /translation="MSEVVRVEGRVWRPESSGAVPWCFFEELSKNRWRPDTKWAESLD
                     WEQIRPQCVHVLEPLPVKLGDWWIEPYCCLAGTVIFYSTVAGARVEVARCDGPHRMWP
                     RISFAERAGVLYEVVGGKVVHVFDRWQHEWRAFKNTRLARHFNGVCGGCEYCDLLREE
                     PA"
     gene            complement(1668..1910)
                     /locus_tag="AC16CX_06610"
     CDS             complement(1668..1910)
                     /locus_tag="AC16CX_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012222.1"
                     /translation="MKRKPEAGQCRFCGCTETTACTIFRIVVRWNCLASFHQTTCTWA
                     DASRTVCTNPDCLRLREGARHPAIRRELRRARESKR"
     gene            complement(1907..2356)
                     /locus_tag="AC16CX_06615"
     CDS             complement(1907..2356)
                     /locus_tag="AC16CX_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012223.1"
                     /translation="MQFALGWTERVEEIDRRLEVAMRSSRLGPFERDVLAVIAMHRGC
                     RNPVASESIAAQLRMEWCENSRRAIAGAVENLVLLEKVPIGGRRGNPHGYFLVMTAED
                     LNLAIHARWSEVYAHLRYLRALTSKSDVAKLFGQGMLQLDRDGKEAA"
     gene            complement(2368..2499)
                     /locus_tag="AC16CX_06620"
     CDS             complement(2368..2499)
                     /locus_tag="AC16CX_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012224.1"
                     /translation="MKTIKLATGARPLVRRCRVRPPHEPHRWRGKKTRYRHGYYCEG"
     gene            complement(2516..2908)
                     /locus_tag="AC16CX_06625"
     CDS             complement(2516..2908)
                     /locus_tag="AC16CX_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012225.1"
                     /translation="MNGEGMCQWCFAGQPHPSDAAAQRQQQKVAEGKAKFEEATMRPP
                     TPEAVKVQVREDGMAGLTGKAIAEKRHLSLSTVNRILAASNGRRQRRRVRTNGVVTIT
                     ATPELVDRIWSGLSLENQAALINKLTEL"
     gene            complement(3521..3862)
                     /locus_tag="AC16CX_06630"
     CDS             complement(3521..3862)
                     /locus_tag="AC16CX_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012226.1"
                     /translation="MSWALLLKAAEVLLLLVLGIVAGAIGLVVAAVCIGFALWAVLWF
                     VLYISSAARVWRMQGLHPRVRFERATAQFQSGRMSIGAYRDEIVRWEAWRQHAEGWQQ
                     TLGYAPKGRPN"
     gene            complement(3859..4233)
                     /locus_tag="AC16CX_06635"
     CDS             complement(3859..4233)
                     /locus_tag="AC16CX_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012227.1"
                     /translation="MSEQLMDAQGKTVEERFAAIRDFFLDNLHLAGMSRTELYNRPKP
                     GEAREPTEEDIVRRWAELVAVPLGDIFIGIQRAFAFATERGQIVTSFRYCVPQIQRRL
                     AEVREARAGLAPRMARMKERQA"
     gene            complement(4230..4667)
                     /locus_tag="AC16CX_06640"
     CDS             complement(4230..4667)
                     /locus_tag="AC16CX_06640"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HIR3012228.1"
                     /translation="MAATVVAALLAVPARGQEAIKFHLGKEQPVILLKVKVNGVERVA
                     LLDTGGSRTIITPAAAGMSKKDLGASPLTTLGGGKTAMAYRAVRLELPGKRWNGLRVI
                     VTDLETVSQNVGEPVELVLGMDVLSQFRAFRVDFKKGVLELEP"
     gene            complement(4694..5176)
                     /locus_tag="AC16CX_06645"
     CDS             complement(4694..5176)
                     /locus_tag="AC16CX_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012229.1"
                     /translation="MAEIKTLAAWKIDQVAALYLEAQAAVEAANREASARRARLIELT
                     REYGSTAPRAWKTRRLCGSDYEIRVSEAQEITVDMRAAMRVKHACIRSGTGRLFRRLF
                     RKVETFILAPGAERLLDQARLPEDAPRNLRALFARAVRVRELAPHVEVGKREKPEEAA
                     "
     gene            complement(5169..5495)
                     /locus_tag="AC16CX_06650"
     CDS             complement(5169..5495)
                     /locus_tag="AC16CX_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012230.1"
                     /translation="MRRGLKRIVGALRGAVGLCPTWRGSLVWRRGPGPDGRMGFECLD
                     CFTFLPLSDELLERSAIALRMARQQRRLIAHRRARRQTRDVAELERLFQLSSVSSARK
                     KDDYHG"
     gene            complement(5492..5731)
                     /locus_tag="AC16CX_06655"
     CDS             complement(5492..5731)
                     /locus_tag="AC16CX_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012231.1"
                     /translation="MNGQEEGSRKVRCIDELRPEYVDAAEARRRRLARQQFWARIYAG
                     RERRNRLYGWGAALLCAIAGIVLAVIALDLLGRSR"
     gene            complement(5728..6717)
                     /locus_tag="AC16CX_06660"
     CDS             complement(5728..6717)
                     /locus_tag="AC16CX_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3012232.1"
                     /translation="MRISENTRRRLADVPPTVQVQAALREYMAKTDLSYPEVGERIGY
                     AHESVRIFLAGDYHLVARDDLMIRAAAWEYLQRNPIVPSQVRVSGPLFVTENYRRIRK
                     YFLSAVERHEICLLYGPPGTQKTFVLEHLVAERNREKKNDALYVYASARMTRLAVLKR
                     TARAAGATVCTLTIDRLINGLVHHFRGRPGPPAIVVDEAQHLTVDALEVLRELYDRSG
                     CGLMLAGSHNLFENFVRGRAQLEQWLSRIDHKDPLPGLLETEVREIAVRQLGNGQPAK
                     LGEKQVRALVEACRVDDIFARDAAGKPTPRKYLSVRRLVKVLEQSKARREQVA"
     gene            complement(6717..>7151)
                     /locus_tag="AC16CX_06665"
     CDS             complement(6717..>7151)
                     /locus_tag="AC16CX_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012233.1"
                     /translation="RVLRDPYDLTQAVARDPETRQFVGELRLQQLVAQGAHGQISRED
                     INAAMCRPRGLKRAYSEYLGALAAISTRLGWQTELEALLDRAGIQRAQLHTGTDGARC
                     LPAPKGVPGARLALPERPRQELSSPFVDDAVEKVVAAFEEET"
BASE COUNT         1149 a         2662 c         2008 g         1332 t
ORIGIN      
        1 actcatcggc ctttttgccc gattcggtca cctgcacgac ttcctcgatc cagcccttca
       61 tcgcgtcgcg gtccgtcttc ccgatcgtca gccccgcgat caggttctcg cacatcgtca
      121 ggacgtcctt ctgaaccgtc gcatatcgct gcgagattaa ggtcgcgccg taaccgttgt
      181 tccgccccag ccgactgagc cggtccactg ccgccaaaga cctcttttgc tcacccatcg
      241 gcctctgcgg tacgaactcc ggcgcctcct cgagaaaaat gtggtgcgga accgccggcg
      301 tcagttccat cagccggtcg caaaaatcgg ccacaaattt ccgccaggtg ttcttcgact
      361 cgagcttcaa gtcgattacg cagcacaggt tttcctgaag cacggcctcg gcgatccgcg
      421 gtccctcgtc cttctcgagc ggcaggtctc cgtgcggtcc gccgaccacg aggaccggat
      481 agccgcccgg cgtgccgtcc ggattgcacc gcagtcccca ccacacgctc gtggggtcga
      541 aggcgatcca cggaatcccg cactcgcaca tctcttcggc gatcaccgtt gccgtcaccg
      601 tcttgcccca gccccgtatg ccgatgatgg cgagcgtgcg ggtcacgaaa tcgagcggca
      661 gggtgaagtt ccgcccgctc tcgagcgtcc ccagttccaa ctcgtgcttc attggatctc
      721 cggatcaacg aatcgcaggc cgccctgggt cagccgctcg gtatggatcc gcagccaatc
      781 caccgcctca tcgtaagcct cggcaaaacc ttcgctgaac gtccgccaga gtttccgttt
      841 cacctcgagc ggcaacgctg catagcaccg cccacagaac gcgtgtttcg gctccttgaa
      901 tccgccgcag gcgcactgcg cggatgccag cgcctcaacc gccttccgct caagcgacgg
      961 cgtcaggctc atgcgtcctc gtcctcctcg acaagttcgt cctcctcgcc ctcaatttcc
     1021 ccggccgcca cttcctggca ggtgcagcac aacagatcgt cgctgcactc cgagcaaccg
     1081 tcctggcagc acccctcggg gcattcgccc tcgagatcca gcggcgcgcc gcaacaccag
     1141 caggcatcag cggtcatgcc ggctcctcac gcagaaggtc gcaatattcg cagccgccgc
     1201 aaacgccgtt gaagtgccgg gcgagcctgg tgttcttgaa agctcgccac tcgtgctgcc
     1261 atcggtcgaa gacgtgcacg accttcccgc ccacgacctc gtagagcacc ccggcgcgct
     1321 cggcgaagga aatgcgcggc cacatccggt gcggcccatc gcagcgggcc acttccacgc
     1381 gggcgcctgc aaccgtgctg tagaagatga cggtgcctgc caggcaacag tacggttcga
     1441 tccaccaatc tcccagcttg acgggcagcg gctccaggac gtgcacgcac tgcggcctga
     1501 tctgttccca gtccaggctc tccgcccatt tcgtatctgg ccgccatctg ttcttcgaca
     1561 gttcctcgaa gaagcaccat ggaacggcgc cggaggactc cggcctccag acgcgaccct
     1621 cgaccctgac gacctcgctc acaccgcctc cttgtctatc tgcacaatca gcgtttcgat
     1681 tcccgcgccc tccgcagttc ccgccggatc gccggatgcc gtgcgccctc acgcagccgc
     1741 aggcagtcgg gatttgtgca gacagtccgg ctcgcgtctg cccaggtgca ggtcgtctgg
     1801 tgaaaactcg cgaggcagtt ccaccgcacg acgatcctga agatcgtgca tgccgtcgtc
     1861 tccgtgcatc cgcagaagcg gcactgcccg gcctcgggtt tccgtttcac gccgcctcct
     1921 tgccgtcgcg gtcgagctgc aacatcccct gtccgaataa cttcgcgacg tcgctctttg
     1981 acgtgagcgc ccgcaagtac cgtaggtgcg catacacctc gctccaccgc gcatggatcg
     2041 ccaggttcag gtcctccgcc gtcatcacga ggaaatatcc gtgcgggttt ccgcgccgcc
     2101 cgcctatcgg caccttctcg agcagcacca ggttctcgac tgctccggca atcgcccgcc
     2161 gcgagttctc gcaccattcc atgcggagtt gcgcggcgat cgactcgctc gctaccggat
     2221 tgcgacagcc tcggtgcatc gcgatcaccg ccagtacgtc ccgctcgaac ggccccagcc
     2281 ggctcgaccg catcgccacc tcgagccgcc ggtcgatctc ctcgacccgc tccgtccacc
     2341 ccagtgcgaa ttgcatcgcc tccggcatca cccttcgcag tagtacccgt ggcgatagcg
     2401 cgtcttcttg ccacgccacc gatgcggctc gtgcggcggt ctgaccctgc accgccggac
     2461 cagggggcgc gccccggtcg cgagtttgat tgttttcatt gaatcgcctc cggcattaga
     2521 gctcggtgag tttgttgatc agtgccgcct ggttctcgag ggacagtccg ctccagattc
     2581 tgtccaccag ctcgggcgtt gccgtgatcg tgacgacgcc attcgtccgg acccggcggc
     2641 gctgccgccg gccgttactg gccgccagga tcctgttgac ggtcgagagc gagagatgac
     2701 gcttctctgc gatggccttg ccggtcagtc ctgccatccc atcctctcgg acctggacct
     2761 tcaccgcctc tggtgttggt ggcctcatgg ttgcctcctc aaatttcgct ttcccctcgg
     2821 cgaccttctg ctgctgccgt tgcgccgcag catcggaagg atgcggctgt cctgcaaaac
     2881 accactggca cattccttcg ccgttcacga tccggtgtgc gacctggacc cgcccgcgtt
     2941 cttggcatgg tccgcagagc atcagcagcc cccgcaccac ggcagcagca cttcgaccgt
     3001 cttcgcgccg atctcgacgt tcctcggcgt gtaccaccgt gtcggtctcc cgcagttaaa
     3061 gcacttcatc gtcagtctca gttgcttcgc gatccggcgc gggatcagga cggtcgctgg
     3121 cccaggcaat ctcgcgccag ggtgcggagc cgagtggtcc tgcgggaagc cccgtttccg
     3181 cctcgtccag agccgcctgt aaagccgccg gaactccgag gatgcccagc accccgggta
     3241 agtcctcaag agcggcactg cggcgctcac cgagcgggcc ccgccattgt caggttcggc
     3301 cgcagcatct cgggctcccg taggtaggta tcgtcgcact cgacgcaccg cgtccttccg
     3361 tcgcggttga tgtaccagaa gtgcatcgtc cggcattcgg ggcacctcac tgcatccttt
     3421 agcaggatgc ccacagcgcc gtcggtgagc caccaactct ccacgaccgc gcggatctcg
     3481 agctgcacca cccggtccag caacacacac ccctgcccgc tcaattcggc cttcctttgg
     3541 gcgcgtaccc aagcgtttgc tgccagcctt cggcgtgttg cctccaggcc tcccaccgga
     3601 cgatctcgtc gcggtaggct ccgatgctca tccttccgct ctgaaactga gccgtggccc
     3661 tctcgaagcg aactcgcgga tggagtccct gcatccgcca gactcgcgcc gctgacgaga
     3721 tgtacaacac gaaccacagc accgcccaca gtgcgaaccc gatgcagacc gccgccacga
     3781 ccaatccgat cgccccggcg acgatcccca ggacgagcaa cagcagcact tccgccgcct
     3841 tgagcagcaa tgcccagctc acgcctgcct ctctttcatt cgcgccatcc gcggcgccag
     3901 tcccgcccgg gcctcgcgca cctccgccag gcgccgctgg atctgcggca cgcaatatcg
     3961 gaagctcgtc acgatctgcc cgcgctccgt ggcgaaggcg aatgcccgct ggatcccgat
     4021 gaatatgtcg ccgagcggca cggccaccaa ctccgcccag cgccgtacga tatcctcctc
     4081 ggtcggctcg cgcgcctcac cgggtttcgg ccggttgtaa agttccgtcc gactcatgcc
     4141 ggccaggtgc agattgtcca aaaagaaatc acggatcgcg gcaaaccgct cctccactgt
     4201 cttcccctgc gcatccatca actgttcgct cacggttcga gttccagtac tcccttcttg
     4261 aagtccacac ggaatgcccg gaactgcgac aacacgtcca tcccgagcac cagctccacc
     4321 ggctcgccta cgttctgcga aaccgtttca aggtcggtca cgattaccct caacccgttc
     4381 catcgcttgc cgggtagttc caggcggacg gcgcggtagg ccatcgcggt cttgccgccg
     4441 cccagggtcg tcaatggcga ggcgccgagg tccttcttgc tcatgccagc ggcagcagga
     4501 gttatgatcg tccggctgcc tccggtgtct aggagggcca cgcgctccac cccattcacc
     4561 ttgaccttca gcaggatcac cggctgctct ttgccgaggt ggaacttgat cgcctcctgg
     4621 ccccgcgctg gtactgcgag gagcgccgca accaccgtcg ctgccatggc cacccgagtt
     4681 gtcccgcccc agttcacgcc gcctcttccg gcttctccct cttcccgact tcaacgtgcg
     4741 gcgcgagctc gcgtacccgc accgcccggg caaacagtgc ccgcaagttc cgcggtgcgt
     4801 cctccggcag cctcgcctgg tccaacagac gctccgcgcc cggcgccaga atgaatgtct
     4861 cgacctttcg gaacagtcgg cggaaaagtc tcccggtgcc cgaccggatg cacgcgtgct
     4921 tcactcgcat cgccgcgcgc atgtcgacgg tgatctcctg cgcctcactg acacgaattt
     4981 cgtaatctga cccgcagagc cgcctcgttt tccacgcacg cggagccgtg gatccgtatt
     5041 cccgcgtcaa ctcgatgagt cgcgcacgcc gcgctgaggc ctcgcggttc gccgcttcga
     5101 cagccgcctg cgcctcgagg tacagcgccg ccacctggtc aatcttccac gccgcgagcg
     5161 ttttgatttc agccatggta gtcatccttc tttctggcgg aggaaacgga agacaattga
     5221 aacagccgct cgagctcggc gacatcgcgg gtttgccggc gcgcgcgccg gtgggcgatc
     5281 aaccgccgct gctgccgcgc catacgcaat gcgatcgccg accgctcgag cagttcatcg
     5341 ctcagcggca ggaaggtgaa gcagtccagg cactcgaacc ccattcgccc gtcgggccct
     5401 ggcccgcgcc gccacacgag cgaaccgcgc caggtcggac acagccccac cgcgccccgc
     5461 agggccccga caatgcgctt gagtcctcgc ctcatcggct ccgccccagc aggtcgaggg
     5521 cgatcaccgc cagcacaatt ccggcgatcg cacagagcag tgccgcgccc catccgtaca
     5581 gccggtttcg ccgctcgcgt ccggcataga tccgcgccca gaattgttgc cgcgccagcc
     5641 gccgccgccg cgcctccgcc gcgtccacat actcgggccg cagctcgtca atgcaccgca
     5701 ccttccggct cccctcctcc tgcccattca tgcgacttgc tcccttctcg ccttcgactg
     5761 ctcgagcacc ttcaccagac ggcgcactga gaggtatttc cggggtgtgg gctttccggc
     5821 tgcgtcccgc gcaaagatgt cgtccacgcg gcaggcctcg accagggcgc gcacttgttt
     5881 ctcgccgagt tttgccggct ggccgtttcc aagttgtcgc acggcgatct cccgcacttc
     5941 ggtttcgagc aatcccggca gaggatcctt gtggtcgatc cgcgagagcc actgctcgag
     6001 ctgcgcgcgc ccgcgcacga agttctcgaa gagattgtgc gaacccgcca gcatcaatcc
     6061 gcacccggac cggtcgtaga gttcccgcag cacctcgagc gcgtccacag tcaggtgctg
     6121 tgcctcgtcc accacaatcg ccggcggccc cgggcggccc cgaaagtggt gaacgagacc
     6181 gttgatcagc cggtcgatgg tcagcgtgca caccgtcgct ccggcagccc gcgccgtgcg
     6241 tttcagcacc gccagacgcg tcatccgtgc cgaggcatag acatagagcg catcgttctt
     6301 cttctcgcgg ttgcgttcgg ccaccagatg ctcgagcacg aacgtcttct gtgttcccgg
     6361 cggcccatac agcaggcaga tctcgtggcg ttcgaccgca gacagaaaat atttgcggat
     6421 ccgccggtag ttctccgtga cgaagagcgg gccggagact cggacctgcg atggcacaat
     6481 cggattccgt tgcaggtatt cccaagccgc cgcccggatc atcaggtcgt cccgcgccac
     6541 caggtggtag tccccggcga ggaatatccg caccgactcg tgggcatacc cgattcgctc
     6601 gccgacctcg gggtacgaca ggtcagtctt ggccatgtac tcccgaagcg cagcctgcac
     6661 ctgcaccgtc ggcggcacgt ccgccaggcg ccgccgcgtg ttttcgctga tccgcatcag
     6721 gtttcctcct cgaacgcggc cacgaccttc tctacggcgt catccacgaa cggcgagctc
     6781 agctcctgtc gtggtcgctc cggcagcgcc agccgcgcgc ccggcacccc ttttggcgcc
     6841 ggtaggcatc tggccccatc cgtgccggtg tggagttgcg cccgctggat tcccgcacgg
     6901 tcgagcagtg cctcgagttc tgtctgccag ccgagccgtg tcgagatcgc cgcgagtgcc
     6961 ccgagatatt ccgagtaagc gcgcttcagg ccgcgtggcc ggcacatggc cgcgttgatg
     7021 tcctcgcggc tgatctgtcc gtgcgccccc tgcgccacga gttgctgcag ccgcagttcg
     7081 ccgacaaact gccgcgtctc ggggtccctg gccaccgcct gcgtcaggtc gtacggatca
     7141 cggagcacgc g
//
LOCUS       DBNGWZ010000223        12575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2416316, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000223 DBNGWZ010000000
VERSION     DBNGWZ010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2416316"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16CX_06670"
     CDS             <1..285
                     /locus_tag="AC16CX_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3012234.1"
                     /translation="ERRPTGEIYNPELAERFPGRDWILTRILWLSGCERGYNRLGKTD
                     TMRRAIYIHGSPDSAEMGKPGSHGCIRMRNQDIVELFELVPVRTVVEIRE"
     gene            551..1291
                     /gene="fabG"
                     /locus_tag="AC16CX_06675"
     CDS             551..1291
                     /gene="fabG"
                     /locus_tag="AC16CX_06675"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3012235.1"
                     /translation="MRLAGKVAIITGAGRGIGQATALKFAEEGAKVVVCDVSPEWIED
                     TVERITHLGGEAMGYTADVRNMGSLQAMVDATVERWGRVDCLVNNAGIVMDAQLKNMT
                     DEQFDQVIEINLKGVYNCTRAVVDTMLKQQSGVILNASSIVGLYGNFGQTNYAASKFG
                     VIGMVKTWARELGRKGIRANAVCPGFIATSILKSMPDRVLRALEDKVPLGRLGKPEEI
                     ANTFAFLASDEASYINGAVIEVSGGLTL"
     gene            1436..1924
                     /locus_tag="AC16CX_06680"
     CDS             1436..1924
                     /locus_tag="AC16CX_06680"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3012236.1"
                     /translation="MSEKPPFTVKPVYWNASEKMLRAIRITVFVEEQGVPQDLEWDGL
                     DEHAFHVMAFAADGTPIGTGRLLQDGHIGRVAVLREWRGKGVGRALLDMLLVIANKMG
                     YDVVKLHAQTRVLDFYLSRGFKTEGEEFMEAGIPHVLMTRTTADQTSWPEAFVTRPLA
                     RM"
     gene            complement(1873..2685)
                     /locus_tag="AC16CX_06685"
     CDS             complement(1873..2685)
                     /locus_tag="AC16CX_06685"
                     /inference="COORDINATES: protein motif:HMM:NF017551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007030 - Golgi organization [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HIR3012237.1"
                     /translation="MCLILLAWHAHPEYPLIFAANRDEAYERPSAASDFWKDEPDIFG
                     GRDLEKGGTWLGLTLAGRIAAVTNYRDGPAARTAPRSRGKLTADFLRGTDEPASYLAK
                     VAPDAAQYGGFSLLVGDHERLYSCSNRGSGIEELRPGVHGLSNHLLDTPWPKVTRGKQ
                     RLSTLLKANEAGLIKGLFEALEDRTAAPDTELPDTGVGLQRERELSPAFVAGERYGTR
                     ASTVLLVSHGNEVTFVERGFGPGGAPQGEVTRRFALHMRARGRVTKASGQDV"
     gene            complement(2852..3133)
                     /locus_tag="AC16CX_06690"
     CDS             complement(2852..3133)
                     /locus_tag="AC16CX_06690"
                     /inference="COORDINATES: protein motif:HMM:NF027614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HIR3012238.1"
                     /translation="MAFSYYIYYRVDSAKAAACEPRIRELFTSVHKATGISGQLMRKR
                     GESNLWMEIYLNVVDDAKFEWELADAVGRLKVQEFLLPGTPRHMEVFEH"
     gene            3238..3816
                     /locus_tag="AC16CX_06695"
     CDS             3238..3816
                     /locus_tag="AC16CX_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012239.1"
                     /translation="MTTRKLVPVLALILLGGCMGPEATSQLNTEVQAVKDRVSQAREE
                     RKSYGKGSIVYDLISLCLAIHEQTLAMLEQRRAAGSWRTQLSYTVNGKPWTTPGDVEA
                     KMSSVEARLKNARAGRESDLQLAKESDDAFRPLLLMSAATKAILISQLEYQLAAHRFG
                     FPSYYVPFHPPAEGATPPQSITAPVGKAGVVQ"
     gene            3844..4047
                     /locus_tag="AC16CX_06700"
     CDS             3844..4047
                     /locus_tag="AC16CX_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012240.1"
                     /translation="MEGNTSQVVVTDIKIPFWSLVVLLVKWALAAIPAVIILIVIGAV
                     ASVVLNLLFGGLFGWHWGAGRMM"
     gene            complement(4120..4407)
                     /locus_tag="AC16CX_06705"
     CDS             complement(4120..4407)
                     /locus_tag="AC16CX_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012241.1"
                     /translation="MHRIAPVMLLAFLLIAYGRPAAAADQPSETDRVRVQISQSKERG
                     KSADAQKKGQKNETRRGATPATPATPATPADPGTSPAIPATPATPATPPSK"
     gene            complement(4714..5304)
                     /locus_tag="AC16CX_06710"
     CDS             complement(4714..5304)
                     /locus_tag="AC16CX_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012242.1"
                     /translation="MELIPLWIKLAYTVFVAATVAVYAVRYPLWNFLWFSDIALIVTV
                     PALWLESSLLASAMLVGVLLPELFWNASFFWRLLAGRRFSGMTDYMFDPGKPLYLRAI
                     SLFHIFLPVLLLWMIVRLGYDPLAPVAQTGLAWVVLPLTYWQADPKVENVNWVFGWGS
                     KPQTRMPPLAYLGLVMIGFPVLIYIPTHLLVQALLG"
     gene            complement(5431..6351)
                     /gene="ygfZ"
                     /locus_tag="AC16CX_06715"
     CDS             complement(5431..6351)
                     /gene="ygfZ"
                     /locus_tag="AC16CX_06715"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3012243.1"
                     /translation="MFCDISQQGLLRFSGEDARLFLHNQLSCDVNTVTVGRSTYGSYC
                     TPKGRMLASFLLWRSEQGFFMQLPSSLREGIQKQISKFILRSKVMAADASGDWTIIGI
                     DSMHAAALVQRAVGGVPRDVHEVIYTQDAMVIRLPGDRYEIIVGRDRSPVVVGSLSDG
                     AGKADQEHWEWLDIRAGVPVILPSTQEEFVPQMVNLDLIGGVSFEKGCYPGQEIVSRM
                     HYRGTLKQRMYLANIASGERPQPADKLYSAHFGEQACGTIVNAARSPEGGHDVLAVIQ
                     IASTERGEVHWKSLDGPALKLMTLPYPVTA"
     gene            6657..8807
                     /locus_tag="AC16CX_06720"
     CDS             6657..8807
                     /locus_tag="AC16CX_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HIR3012244.1"
                     /translation="MLAAETYIWGLQGICQLHRIPFAPNLVLQQFPPPYGLTSLQQAA
                     TAFGLKSGLRDARLDELPELPAPFVAVLKPSEISTNTAPGDGASAEEKSVQLHRLVIV
                     VKYAGGGISYFERGRQHPVTAPFEEFGTQFAGKVLLCVPAAPELKTEDAAAIGSREFG
                     FRWFVPELFRHKVIWRDVLLASLAIQLMALATPVFTQVVIDKVIVHHTTSTLVVIGIA
                     LGVFIVFTAVMSWVRQYLVLHTGNRIDAVLGSQVFEHLFRLPPRYFERRPTGVLVARV
                     HGVETIREFLSSAAVTLVLDVPFLLIFLAVMFYYSVTLTLVTMAILSLIVGLSLFVAP
                     LFRQRLNHQFLLGARNQAFLTEYVSGMETVKSLQMEPQLNKRYGDYLATYLQSGFGTR
                     QIANSYNVAANMLEQLLTLIILCVGAWLVMTSQEFTIGMLVAFQMFANRLSQPMLHLV
                     ALWQQFQQADIAVRRLGDVMNAPAEPYSLLPAREAKGEGWIEIRALSFRYATNLPYLY
                     KTFSLGIEPGKCVAIMGPSGSGKSTLAKLLQGFYQPEEGGIVIDGHDIRHLSANELRQ
                     HFGVVPQETTLFSGTIYDNLVLASPQASFEQVIQACKLAEIHETIEQLPQGYQTEVGE
                     HGAGLSGGQKQRIAIARALLKRPRILIFDEATSSLDAPTAEQFARTVNQFRGRVTMLF
                     IAHQLPKGLQVDNVVTLGQWGTRMGVVGEERKDA"
     gene            8881..10218
                     /locus_tag="AC16CX_06725"
     CDS             8881..10218
                     /locus_tag="AC16CX_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HIR3012245.1"
                     /translation="MSLKSEAVEFAPEIIKAQHQPPSPLPRMVLYALLVLFAVMLVWA
                     FAGRLDIVAVAQGKLVPQSFLKIVQPAESGIVREILVKEGEEVREGQVLVRMDTRLSE
                     ADSKTLQAELHRKRLQLRRIEAELAGAPLKRQADDPAEIYTQIEAQYQARRQAYLDAL
                     GAEQATLLKAQHDVKSATEIEGKLQKTVPIYKDQAESWDKLAKEGFAGRLLALERQRT
                     YLESEQDLRAQIQNIASLRALIAQSEKRIAQITSNYRQQLQNERAEAEALYHKLQQEW
                     DKQQHRHSLLELKAPQAGIVKDLATHTPGTVVAPGTILLTLVPHDEPLVAEVWVNNID
                     AGFVLTNQKARVKLAAYPFQKYGMLDGIVRQISADAQEKSEGANSLVKPLQEAAYRAL
                     ISLNKDYLENQGQRLRLVPGMQVSAEIHLGTRSVIEYLLSPVQRVAHEAGRER"
     gene            complement(10723..11973)
                     /locus_tag="AC16CX_06730"
     CDS             complement(10723..11973)
                     /locus_tag="AC16CX_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3012246.1"
                     /translation="MEDPNIALRFAVAIGLGMLLGLERERTKSEEGGLGVRTFALIAL
                     AGATAGYLDESLGLSAFALVLFIAVATLIVSLYLVTAVRGDTGVTTESSALLCFMLGV
                     LCARGQVQFAAWVAVAMALLLALKEWLHRLARRIEASDVEATLKFAIVTLIILPLVPD
                     QNYGPAPLNVLNPYKIWLMVVLISGLNFASYLLIKIVGAEHGIGIAGLLGGLVSSTAV
                     TLGFSQRSRQPGEDASALALGILLAWTVMFFRVVIMSWLISGALGLRLAIAMGLLCAV
                     SLGACYWLWRRRQQQERGQVEAGHNPFELDQAIKFGLLFGVVVFIAKAAQVYLGDAGL
                     YLAAGVAGLTDVDAITLAMADLARSDEQNVTIAARAILIAALANTLVKSGMAVSLGSQ
                     ELRRLALPISGLLFAAGVAAAFLI"
     gene            12082..12375
                     /locus_tag="AC16CX_06735"
     CDS             12082..12375
                     /locus_tag="AC16CX_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012247.1"
                     /translation="MANNLFVSCELFRPEQNYDRVAAAVKSVGDAWTKVQFSLWYLKT
                     PLSARQVCDRVKPALDKNDQLVVIDATNNKVAWINLGAEVSGSLHDQGFQTVA"
BASE COUNT         2557 a         3769 c         3953 g         2296 t
ORIGIN      
        1 gagcggcgcc caacgggaga aatctacaac ccggaactgg ctgagcgctt ccccgggcgc
       61 gactggatac tcacccgcat tctctggctg tcgggctgcg agcggggata caaccgtctg
      121 ggcaagacgg acaccatgcg tcgcgccatc tacatccacg gcagccctga cagcgctgag
      181 atgggcaagc cgggatcgca cggctgtatt cgcatgcgaa accaggacat cgtcgagctg
      241 ttcgaacttg tgccggttcg tacagtggtc gaaatccgtg aataggcggg cctgaggcgg
      301 gtccggcgac ccgtcttatc ccgcgttgcc agataatgaa gccggtctcg gacattccaa
      361 acatccaatg ggttaagggt ctgccttaac tctgaaacca tgattattgt gcgatgcgcc
      421 aagataggtt aaattggcgc aactcatagc ttgagggtcg atccgggtca tggctgataa
      481 acggcgcggc ggcaacgggc acggcaacgg gagcagtaaa ggcaacggaa acggcaacgg
      541 caatgcgacc atgagattgg ccggcaaggt ggcgatcatc accggagccg gcagggggat
      601 cgggcaggcg acagcgctga agttcgcgga ggaaggcgcc aaagtcgtgg tctgtgacgt
      661 gagcccggag tggatcgagg acaccgtcga aagaatcacc cacctgggcg gcgaggcgat
      721 gggctatacc gccgacgtcc gcaacatggg atcactgcaa gccatggtgg atgccaccgt
      781 cgagcgctgg ggcagggtcg attgcctcgt gaacaacgcc ggcatcgtga tggatgcaca
      841 gctcaagaac atgactgacg agcagttcga ccaggtcatc gagatcaatc tcaagggggt
      901 ctacaactgc acgcgggcgg tggtggatac catgctcaag cagcagtccg gcgtcatcct
      961 caacgcttct tccatcgtcg gcctgtacgg caacttcggc cagaccaact acgcggcgag
     1021 caagttcggc gtcatcggca tggtcaagac ctgggcgcgg gagctcgggc gcaaaggcat
     1081 ccgcgccaac gcggtctgcc ccggcttcat cgcgacctcg atcctgaaat cgatgcccga
     1141 tcgcgtgctg cgcgcgctgg aagacaaggt cccgctcggg cgtctgggca agcccgagga
     1201 gatcgccaac accttcgcct ttctcgcctc ggacgaggcg agctacatca acggcgcagt
     1261 gatcgaagta agcggcggcc tcacgctgta aagcgtggcc ggtgccggta agcggcatag
     1321 tgcgccgtgg cggtggtcgt acggatggtc cgagggccgc tcacgatgtc ccggccacgg
     1381 aaaccgagct ggcgccgaca cgatcgaacc gactcccggg aatataataa cttccgtgag
     1441 cgagaagcct cccttcaccg tcaagccggt ctattggaac gccagcgaga agatgctgcg
     1501 cgcgatccgc atcaccgtgt tcgtcgagga gcagggcgtg cctcaggatc tggaatggga
     1561 cgggctcgac gagcacgcct tccacgtcat ggcgtttgcg gcggacggca cgccgatcgg
     1621 caccggccgg ctgttgcagg acggccacat cgggcgcgtg gcggtgctca gggaatggcg
     1681 cggcaagggg gtgggcaggg ccctgctcga catgctgctg gtcatcgcca acaagatggg
     1741 ctatgacgtc gtgaaactgc atgcccagac ccgagtcctg gatttttacc tcagccgggg
     1801 tttcaagacg gagggcgagg agttcatgga ggcgggcatt ccccacgtgc tgatgacgcg
     1861 cacgacggcc gatcagacgt cctggccgga ggccttcgtg acgcgccccc tggcgcgcat
     1921 gtgaagcgca aaacgccgcg tcacttctcc ctgcggcgcg cccccggggc cgaacccccg
     1981 ctcgacaaaa gtcacctcat tgccgtggct gacgagtaag acggtggaag cgcgcgtgcc
     2041 gtagcgctcg ccggcgacga aagcggggga gagctcgcgc tcccgctgga gacccacgcc
     2101 cgtatcgggc agctccgtgt cgggggccgc ggtgcggtcc tcgagggcct cgaacaggcc
     2161 cttgatcaac cccgcttcat tggccttgag cagggtcgag agccgctgct tgccgcgcgt
     2221 gactttgggc cacggcgtgt cgagcaggtg attgctcaac ccgtgcacgc cggggcgcag
     2281 ttcctcgatg ccggagccgc ggttggagca gctgtacagg cgctcgtggt ctccgacgag
     2341 cagggagaat ccgccgtatt gggccgcgtc gggcgcgacc tttgcgaggt agctggccgg
     2401 ttcgtcggtc ccgcgcagga aatcggccgt cagtttcccg cgcgagcgcg gcgcggtcct
     2461 ggccgccggc ccatcgcgat agttggtcac cgccgcgatg cggcccgcga gcgtcaggcc
     2521 aagccaggtg ccgcctttct cgagatcgcg gccgccgaag atgtccggct catctttcca
     2581 gaaatccgaa gcggcgctcg ggcgctcgta ggcttcatcc cggttggccg caaaaatcag
     2641 cggatactca ggatgggcgt gccaggccag aagaatcaaa cacatcgtga catggtggag
     2701 cgtgacatgg aagatcgtga cgtggtatag cgtgacatgg taaggcgtgg ctaaaggcga
     2761 attgcttgct gaagttcaaa aacacgcagc atcacgtcac gcttccacat gtcacgccgt
     2821 atcatgtcac ggttcaccat gtcacgcttc atcaatgttc aaagacctcc atgtgccgtg
     2881 gcgtgccggg cagcagaaac tcctgcacct tgagccggcc gactgcatcc gcaagttccc
     2941 attcgaattt agcgtcgtcg acgacgttca ggtaaatttc catccacagg ttcgactcgc
     3001 cgcgctttct catgagctgc ccggaaatac cggtcgcctt gtgcacggac gtgaataact
     3061 cccgaatccg cggctcgcac gcggcggcct tggcggaatc gacgcggtag tagatgtaat
     3121 aggaaaatgc cacggaccta tcctactggc ggtccgccac ggccgcaacc gcgggcgaaa
     3181 ggtgcaggcc cggcgtttct gtaagatagc gccacacggt ttcaaaggag cttcgccatg
     3241 acgactcgca agcttgtccc ggtcctggcc ctgatcctgc tgggtggatg catgggcccc
     3301 gaagccacat ctcaactgaa tacggaagtg caagcggtga aagaccgcgt cagccaggcg
     3361 cgggaagaac gtaaatcgta cggcaagggt tcgatcgttt atgacctgat ctccctgtgc
     3421 ctcgccatcc acgagcagac gcttgccatg ctggagcagc ggcgcgcagc gggcagttgg
     3481 cgcacccaac tgtcctacac ggtgaatgga aagccatgga cgacgccggg cgatgtagag
     3541 gcgaaaatgt cctccgtgga ggcgaggctc aagaacgccc gcgccggccg cgagtccgac
     3601 ctgcaattgg caaaggagtc ggatgatgcg ttccggccgc ttcttctgat gagcgcggcc
     3661 accaaggcga tcctcatctc ccagctcgaa tatcagctgg ccgcccatcg cttcggtttc
     3721 ccgtcgtatt acgtgccatt ccacccgcct gcggaaggcg cgacgcctcc ccagagcatt
     3781 actgctccgg tcggaaaggc cggcgttgtt caatgaaacc cgtgttcgtg aaaggaggct
     3841 tcaatggaag gcaatacgag ccaggtcgtg gttaccgata tcaagattcc cttctggtcc
     3901 ctggtggtgc ttctggtcaa gtgggcgctg gcggcgatac cggcggtgat cattcttatc
     3961 gtcatcggtg cagtcgcatc ggtggtgctc aacctcctgt tcggtggctt gttcggctgg
     4021 cattggggcg cgggccggat gatgtagccg cgggaatggc tgcggaaaaa agcaagggca
     4081 ggcctgcacc tgcccttgcg atccagcgct gacgccctgt tacttgctgg gcggggtggc
     4141 cggagtggcg ggtgttgccg gaatcgcggg ggacgtgccg gggtccgccg gggttgcggg
     4201 agttgccggc gtcgccggtg tggctccacg acgggtctcg ttcttctgac ccttcttctg
     4261 cgcatccgca gattttccgc gctcctttga ctggctgatc tgcacccgca cacgatccgt
     4321 ttccgacggc tggtcagccg cggcggcggg tcggccatac gcgatcagca gaaacgcaag
     4381 cagcatgact ggggcgatcc tgtgcatgat gttttctcct ttcggggtaa aacatccaga
     4441 aactgagcaa ccgccgtacc agttttcccg atgcctaatg ggatcagata gttaacgttc
     4501 acccctggaa tcaccgcatc atcgaatcat gcatcaagct gtcgttcgag tacgacacgg
     4561 cagcaggggc attccgtaaa gcacttgatt cgatgtgtgc tatccgtgtc gtcagcgagc
     4621 gacgaaattc cgcgaggcgc ctggaaccca gccgagcagc gtcttgagca ttatcagaaa
     4681 ggcgtgaata gtgagtcgtg aatgatgagt ggattaaccc aacagcgctt gcaccagcag
     4741 gtgcgtcggg atgtagatca ggacgggaaa cccgatcatt actaggccga ggtacgcaag
     4801 cggcggcatg cgtgtctgtg gtttggagcc ccagccgaat acccagttga cgttctccac
     4861 tttcgggtcc gcctgccagt aggtgagcgg gagcacgacc caggccagcc ccgtttgcgc
     4921 aaccggggcg agcgggtcat acccgagccg tacgatcatc cagagcagca gcaccggcag
     4981 aaatatgtgg aacagcgata tcgcccgcag gtagagcggc ttgccgggat cgaacatgta
     5041 atccgtcatg ccgctgaaac gtctaccggc gagcagccgc cagaagaagc tcgcgttcca
     5101 gaacagctcc ggcagcagca cgcccacgag catcgcgctg gcgagcaaac tgctctccag
     5161 ccagagcgcg ggcaccgtga cgatcagcgc gatgtccgag aaccaaagga agttccagag
     5221 cgggtacctg accgcataga ccgccaccgt cgccgccacg aacaccgtgt aggcgagctt
     5281 gatccagagc gggatcagct ccatgcggcc ggaaagcgtg aatcgtgagt cgtgagtcgt
     5341 gatcgggctt tcacaccgga taccttttcc cccgactgcg ggagaggtct cgggtcagag
     5401 gagatccact caccgttcac tactcaccaa tcacgcagtt acgggatacg gcagggtcat
     5461 cagcttgagc gccggcccat cgagcgattt ccagtgcacc tcgccgcgtt ccgtactggc
     5521 gatctggatg acagcgagta cgtcgtggcc gccctccggc gagcgcgcgg cgttgacgat
     5581 cgtgccgcaa gcctgctcgc cgaaatgcgc gctgtagagc ttgtcagccg gctgagggcg
     5641 ctcgcccgag gcgatgttgg cgagatacat ccgctgcttg agcgtgccgc ggtagtgcat
     5701 gcgcgagacg atctcctggc ccgggtagca gcccttctcg aagctcacgc cgccgatgag
     5761 atcgagattc accatctgtg gcacgaattc ttcctgggtt gaggggagga tcaccggtac
     5821 accggcgcgg atgtcgagcc attcccagtg ctcctgatcc gccttccctg cgccatcgga
     5881 aagcgaaccg acgacaaccg gagacctgtc ccgcccgacg atgatctcat agcgatcgcc
     5941 cggaaggcgg atgaccattg cgtcctgagt gtagatgacc tcgtggacgt cccgcggcac
     6001 gccaccgacc gcacgctgca ccaacgcggc ggcatgcata ctgtcgatcc caatgatcgt
     6061 ccagtcgccg gatgcatctg cggccatcac cttcgagcgc aggatgaatt tcgagatctg
     6121 cttctggatg ccttcgcgaa gggaggaggg caattgcatg aagaagccct gctccgaacg
     6181 ccatagcagg aaactcgcga gcatccggcc cttcggcgtg cagtaactcc cgtacgtgct
     6241 ccggccgacc gttaccgtat tgacgtcgca gctcaactgg ttgtgcagaa aaagccgggc
     6301 gtcctccccc gaaaaacgga ggagcccctg ttgggagatg tcacagaaca ctggctatga
     6361 attcccagga aatagaaaac gccaggttga gaaaaagctt taacaagcgc gaatacccgg
     6421 atgacacaaa atcctaaaga gattactcgg ccgattttag atcataaaac ctatcattat
     6481 gttagatttt ttgctctata acctcatgat tagctgctgt aaataggccc tgtggataaa
     6541 gtcagttcag gcacaatctt acacagacct tgccagcgaa acgctcgtgc gaaggcgcgc
     6601 tgcagggtgt cctgtccgct gaataagaga gagggctgtc tctggctggc gaactgttgt
     6661 tggcggctga gacttacata tgggggctgc aggggatctg tcagcttcac cgcattcctt
     6721 ttgccccgaa cctggtcttg cagcagtttc cccctcctta cggcctgacc tcgctgcagc
     6781 aagcggctac cgcgtttggg ctgaagagcg gattgcgtga cgcgcggctc gatgagctgc
     6841 ccgagttgcc cgcgccattt gtcgccgttc tgaaaccctc cgagatttcc acgaataccg
     6901 caccgggtga tggcgcaagc gcagaagaga agagcgtgca actgcatcgt ctggtgatcg
     6961 tagtcaagta tgccggcggt ggcatttctt actttgagag gggcaggcaa catccggtta
     7021 ccgccccgtt cgaggaattc ggaacccagt tcgctggcaa ggtcctgctg tgcgtgccgg
     7081 ccgctcccga gctgaaaacc gaggacgcgg ccgcaatcgg gtcccgcgaa ttcggcttcc
     7141 gctggtttgt cccggagctg ttccggcaca aggtgatctg gcgcgacgtg ctactggctt
     7201 cgctggctat ccagttgatg gctttggcaa cgcctgtatt cactcaggtg gtgatcgaca
     7261 aggtcatcgt ccatcacacg acgagcacct tggtggtcat cggcatcgcg ctcggcgtgt
     7321 tcatcgtttt caccgccgtg atgagctggg tgcgccagta cctggtgctg cacaccggga
     7381 atcggatcga tgcggtgctc ggctcgcagg tattcgagca tctcttcagg ctgccaccgc
     7441 gctatttcga acgccgcccg acaggcgttc tggtggcgcg ggtgcatggc gtggaaacca
     7501 tcagggagtt cctgagcagc gcggcagtga cgctcgtcct cgacgtgcct ttcctgctca
     7561 ttttcctcgc ggtcatgttc tattacagcg tgacgctcac gctggttacg atggcgatat
     7621 tgtccctgat cgtcggcctg agcttgtttg tggcgccgct gttccgacaa cgcctgaacc
     7681 accaattcct gctgggggca cgcaaccagg cgttcctgac cgagtacgtt tcaggcatgg
     7741 aaacagtaaa gtcgctgcag atggagccac agctcaacaa gcgttacggc gactacctcg
     7801 caacctatct tcagtccggc ttcggcaccc gtcagatcgc caacagctac aacgtcgccg
     7861 ccaatatgct cgaacagctg ttgacgctga tcattctgtg cgtgggcgca tggctggtga
     7921 tgaccagcca agagttcacc atcggtatgc tggttgcatt ccagatgttt gcaaaccggc
     7981 tctcgcaacc gatgctgcac ctcgtcgctc tgtggcagca gttccagcag gccgatatcg
     8041 ctgtaagacg gttgggcgat gtgatgaatg cgcccgccga accttattcg ctgttgcctg
     8101 cgcgggaagc aaagggggaa ggctggatcg agattcgagc actcagcttc cgatacgcga
     8161 ccaacctgcc gtatctctac aagactttca gtttggggat cgagcccgga aaatgcgtcg
     8221 cgatcatggg cccatcgggc agcggcaaga gcacgctggc gaagctcctg caggggttct
     8281 atcagccgga agagggcggc atcgtgatag acgggcacga catccgccat ctgagcgcca
     8341 atgagctgcg ccagcacttc ggtgtcgtgc ctcaggagac caccctgttc agcggcacaa
     8401 tttacgacaa cctggtcctg gccagtccgc aagcgtcctt cgagcaagtc atccaggcct
     8461 gcaagctggc ggagatccac gaaaccatcg agcagctgcc gcaaggctat cagacggaag
     8521 tgggggagca cggtgcaggg ctatcaggcg gccagaagca gcgcatcgcc atcgcgcgcg
     8581 cgctgctcaa gcgcccgcgc atcctgatct tcgacgaggc aaccagcagc ctcgatgcac
     8641 ccaccgccga gcagttcgcc cgtaccgtaa atcagtttcg aggacgggtg acgatgctct
     8701 tcatcgcaca ccagctaccc aagggcttgc aggtggacaa cgtggtgacg ttgggacagt
     8761 ggggaacgcg gatgggtgtg gtgggcgagg agcgcaagga tgcctgattt agtgtccaca
     8821 aaacctggga tggcgcactc cggcgctttt tctggatgga ggaatatcat ggggcacatc
     8881 atgagcttga aatcggaggc ggtggagttc gcgcctgaaa tcatcaaggc acagcatcag
     8941 ccgccgtcac cgctgccgcg catggtgctc tatgcgttgc tggttttgtt tgctgtgatg
     9001 ctggtctggg cgttcgcggg ccggctcgac atcgtcgcgg tggcgcaggg caagctcgtg
     9061 ccgcagagct tcctcaagat cgtgcaaccg gccgaatccg gcatcgtgcg cgagatcctg
     9121 gtcaaggaag gtgaggaggt cagggaagga caggtgctcg tgcgcatgga cacgcggctc
     9181 tcggaggcgg acagcaagac gctgcaggcg gaactgcacc gcaaacgcct acagctgcgg
     9241 cgcattgaag ccgagctggc cggggcaccg ctgaagcggc aggccgacga tcccgccgaa
     9301 atctataccc agatcgaggc gcaataccag gcacggcggc aggcttattt ggatgccttg
     9361 ggggcagagc aggccacact gctaaaggcg caacacgacg tgaaaagcgc gaccgagatc
     9421 gagggcaagc tccagaaaac ggtgccgatc tacaaggacc aggcggaatc ctgggacaag
     9481 ctggcgaagg agggcttcgc ggggcggctg ctggcgctcg aaagacaacg cacctacctc
     9541 gaaagcgaac aggacttgcg ggcacagatc cagaacatcg ccagcctgag agcgctgatc
     9601 gcgcaatcgg aaaaacgcat cgcgcaaatc acctcgaact accgccagca attgcagaac
     9661 gagcgcgcgg aagctgaagc gctctatcac aagttacaac aggaatggga caagcagcag
     9721 caccggcatt ccttgctcga gctgaaggcg ccccaagcgg gcatcgtgaa agatctggcg
     9781 actcacacgc cgggcacggt ggtggctccg ggcacgatat tgttaacctt ggtgccgcac
     9841 gacgagccgc tcgtcgccga agtctgggtg aataacatcg acgccggctt cgttttgacg
     9901 aaccagaaag cgcgcgtgaa gctcgctgca taccccttcc agaaatacgg catgctggac
     9961 gggatcgtgc gccagatcag cgcggacgcc caggaaaaat cggaaggagc caacagtctc
    10021 gtcaagccac tgcaggaagc ggcttaccgc gcgctcatca gcctgaacaa ggattacctc
    10081 gaaaaccagg gccagcgcct gcgcctcgtt cccggcatgc aggtcagcgc cgagatccac
    10141 ctcggcacgc gcagcgtgat cgagtattta ctgtcgcccg tacagagggt cgcgcacgag
    10201 gccggccgtg aacgttaggt caaagccaat atacttgaaa tgggttgaat tcacgtatgt
    10261 aagttatgac acacgggtag ccttgtcccc gcctcccgag acgtagtcgc ttcagctggt
    10321 gcgcgctgcc ccgaacctgc ggtgcgaccg atccaggctc agcccccgcc tgaacgtttt
    10381 gaatgttgct tcgttgcgca tttttggcgc gcgaaggggt ttcctgacca tgcaggtcga
    10441 acgattacat gaagtcaggg gcagtgccag gacaggggtg aaatgtggtc gaaaccgaac
    10501 cgaaatcgct tgacgagctt cgcaaatatg gcgaggagct ctcccggaaa cgggtggtgg
    10561 acgagcccgc ttacaagcgc tgactgctcg ccaaacagac ggtctggctt gctttgctgg
    10621 tggctgcctt cctgattttc tatctgattg atatcctgca ggcgtcgctg gcgctgctcc
    10681 tggacaagtt ttagacagtc tgccggtctt cgttccagcc actcagatca gaaacgccgc
    10741 cgcgacaccg gccgcgaaca gcagtcccga gatcggaagg gcgaggcgcc gcagttcctg
    10801 cgagccaagg ctgacggcca tgccgctctt caccagcgtg ttggcgaggg cggcgatgag
    10861 gatggcgcgc gccgcgatgg tgacgttctg ttcatcgcta cgggcgagat ccgccatcgc
    10921 gagcgtgatc gcgtcaacat cggtcaagcc cgcgacgccc gcggcgaggt agagccccgc
    10981 atccccgagg tagacctggg cggccttggc gatgaacacg acgacgccga acagcagtcc
    11041 gaacttgatc gcctggtcga gttcgaacgg attgtgcccg gcctccacct ggccgcgctc
    11101 ctgctgttgc cgcctccgcc acagccagta gcaggcgccg aggctgaccg cgcatagcag
    11161 ccccatcgcg atggcaaggc gcaggccgag cgccccgctg atcagccacg acatgatcac
    11221 gacgcggaag aacatcacgg tccacgcgag caggatgccg agggccaggg ccgaggcgtc
    11281 ctcgcccggc tggcggctgc gctgcgagaa gccgagcgtc actgccgtgc tcgagaccag
    11341 tccgccgagc agccccgcga tgccgatgcc gtgctcggcg ccgacgatct tgatgagcag
    11401 gtaactcgca aaattgagcc cggagatcag caccaccatc agccagatct tgtaggggtt
    11461 cagtacgttg agcggtgccg ggccgtagtt ctggtcgggc accagcggca ggatgatcag
    11521 ggtgacgatg gcgaacttga gggtcgcctc gacgtcggag gcctcgatgc gacgcgccag
    11581 ccggtgcagc cattccttga gcgcgagcag cagcgccatc gccaccgcca cccaggcggc
    11641 gaactggacc tgcccgcgcg cgcacagcac gccgagcatg aagcagagca gcgccgagga
    11701 ctcggtcgtg acgccggtgt cgccgcgcac ggcggttacc agatacaggc tgacgatgag
    11761 cgtggcaacg gcgatgaaaa gaacgagcgc gaacgcgctc agcccgaggc tctcgtcgag
    11821 gtagcccgcg gtcgcgcccg cgagcgcgat gagagcgaag gtgcgcacgc ccagcccgcc
    11881 ttcctcgctc ttggtgcgct cgcgctcgag cccaagcagc atgccgaggc cgatcgcgac
    11941 cgcgaagcgc agtgcgatat tcgggtcttc catccggcca tcttagcaga cggattcgac
    12001 caggacgcgc ccggaaaaaa tggcgtcatc gtttttgacc tatatcaaac tttccgtgac
    12061 ctgggcgctg tataaaacag catggccaac aacctgtttg tttcgtgcga gttattcagg
    12121 ccggagcaga attacgacag agtggctgcg gcggtcaagt ctgttggcga tgcctggaca
    12181 aaagtccagt tctcgctttg gtacttgaaa accccgctat ccgcccggca ggtctgcgac
    12241 cgggtcaagc cggcgttgga taaaaacgac cagctggtcg tcattgacgc cacgaataac
    12301 aaggtagcct ggatcaatct gggtgcggaa gtttccggca gtctgcacga ccagggcttc
    12361 cagaccgtcg cctgagcagt tttggggcgg gacaatctgg atccctaagt cggcagccgg
    12421 cagcgtaaca cagtacttgc tttgaccggt ccagaacgtc acgcacgagg ccgggcgtga
    12481 acgtctgctg aaatgccggt agatcgccgg ccaccccggg ttcgattcca tttgatatag
    12541 tcgaagatca ggtttgcgcc cgtacgccgc ccagc
//
LOCUS       DBNGWZ010000224         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2666497, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000224 DBNGWZ010000000
VERSION     DBNGWZ010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2666497"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="AC16CX_06740"
     CDS             <1..424
                     /locus_tag="AC16CX_06740"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3012248.1"
                     /translation="VYCYPRTGRPDREVPTGWNEIPGARGCTPQSCAFRDHFEELKRA
                     GAAHVFGLSTQDTAYQREAAERLHLPFELLSDEKLEFARALRLPTFEVAGMTLIMRLT
                     LIARDGVIEKVFYPVFPPDRNASDVLDWLAQQTVSRKR"
     gene            complement(501..1472)
                     /locus_tag="AC16CX_06745"
     CDS             complement(501..1472)
                     /locus_tag="AC16CX_06745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012249.1"
                     /translation="MKKIVLPALASLLVLAAFTATAQQSYPARPIRLIVPYPPGGNVD
                     ITARVIGPTFGEFIGQTIVVDNRSGAGGNLGANLVAKASPDGHTLLMGSSGPLAINPI
                     VIKDTPYDPLKDFTPVSRVHVVPLVVLAGPKSGVGSVKELIERAKAAPGKITVASAGT
                     GTTNHLGIELFSVMAGVKVLHVPYKGSGPALGELLGGQVQFMFDQLTSSIGYIRDGRL
                     KVLAVAGSRRSDVLPGVPTLDESGLKGYEASTFIGVLAPGGTPRAVIEKLNAAMRKVM
                     DTSSVVERFRALGADPGASSPEEFGKTIRDELGKWRDLAQRVGLKFE"
BASE COUNT          289 a          554 c          550 g          293 t
ORIGIN      
        1 cgtgtactgc tacccgcgca ccggccggcc cgaccgggag gtgccgacgg gatggaacga
       61 gatacccggg gcgcgcggct gtacgccgca atcgtgcgcg ttccgcgatc acttcgagga
      121 actcaagcgc gcgggcgcgg cgcacgtatt cggcttgagc actcaggaca ccgcctacca
      181 gcgggaggcg gccgagcggc tgcatttgcc gttcgagctg ctatcggacg agaaacttga
      241 attcgcgcgc gcgctcagac tgccgacctt cgaggtagcc ggtatgacgc tgatcatgcg
      301 cctgacgctg attgcgcgcg acggcgtgat cgagaaggtg ttctacccgg tgttcccgcc
      361 ggaccgcaat gcaagcgacg ttctcgattg gctcgcccaa caaacggtaa gccgtaagcg
      421 gtaatcggta agcagtaagc ggagagagaa tacttctctt cacaattcgg ctagacggct
      481 caccgctaac cgctcaccgc ttactcgaac tttaggccga cgcgctgcgc cagatcgcgc
      541 catttgccga gttcgtcgcg gattgtcttg ccgaattctt cgggagagct tgcaccgggg
      601 tcggcgccca gcgcgcggaa tcgttcgacc acgctggaag tatccatgac cttgcgcatc
      661 gcggcattca gcttctcgat caccgccctc ggggtgccgc ccggcgccag cacgccgatg
      721 aacgtcgagg cctcgtagcc cttcagcccg gattcgtcca gcgtcggaac gcccggcagc
      781 acgtccgagc gccggctgcc cgccacggcc agcaccttga gccggccgtc gcggatgtag
      841 ccgatcgagg aagtgagctg gtcgaacatg aactgcactt gaccgccaag cagctcgccg
      901 agcgccgggc cgctgccctt gtacgggacg tgcagcactt tcacgcccgc catcacgctg
      961 aagagctcga tccccagatg gttggtggtg ccggttcccg ccgaggcgac cgtgatcttg
     1021 ccgggagcgg cctttgcgcg ctcgatcaat tccttcaccg acccgacccc cgacttcggg
     1081 ccggccagca ccaccagcgg cacgacgtgc acgcgcgaga cgggcgtgaa gtccttgagg
     1141 ggatcgtacg gcgtatcctt gatcacgatc gggttgattg cgagcggccc gctcgaaccc
     1201 atcagcaggg tatggccgtc gggcgacgcc ttggccacca gattggcgcc gagattgccg
     1261 ccggcgccgg accggttgtc aacgacgatg gtctgtccga tgaactcgcc gaacgtcggg
     1321 ccgatgacgc gggcggtgat gtcgacgttg ccgccgggcg gatacggcac gatcagccgg
     1381 atggggcgag cggggtaact ctgctgcgcg gtggcggtga acgcagcgag cacgagcaac
     1441 gaggcaagcg caggcaagac gatttttttc atggtacgtt ctcccattga tatcgccacc
     1501 gcgttcctta cactacgcgg cgcttcctga gatcttcgat cgcctgcgcg tcgaacccca
     1561 gctcgcccag tatctcgcgc gtatgctcgc cggcgtcggg cgcgtgagtg cgcaactgcg
     1621 ccggcgtgcg cgagaggttg acgccctggc cgagcagctc ctgctgcccg agcttggggt
     1681 gcacga
//
LOCUS       DBNGWZ010000225         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_527528, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000225 DBNGWZ010000000
VERSION     DBNGWZ010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_527528"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="AC16CX_06750"
     CDS             <1..518
                     /locus_tag="AC16CX_06750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3012250.1"
                     /translation="HLAKRPDVDMSRIVVQGRSWSGYWAAKLAITERSRLRGVVVHGG
                     PIHHYFQPDWLKPSLATGEYLYDYLQAKCAMMGAEGVDELLDRGRGFSLLEAGLLDRP
                     SAPMLVVNGARDTQVPVADAYVLLEHGEAKEAWINPQGGHMGRSAQWPSSAIAEKVLM
                     PWITRRLGQAL"
     gene            553..1632
                     /locus_tag="AC16CX_06755"
     CDS             553..1632
                     /locus_tag="AC16CX_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3012251.1"
                     /translation="MGSTPVKLGIVGLGRWAKVLTRASRQSDKLRIVAGYSRSEDKRR
                     SFQQEFGIPVTPDLKTLLAEPEIKGVILTVPNEQHLPVSEEVARAKKHIYTEKPIANT
                     LEDGLRIAALEKQYGVTVTVGHSARLMAGFRQIRAAIDAGELGRVGFIEANFSNERAL
                     ELTPSTWRWYRSKAPGGPLSQLAIHQFDVLHYLGGEMVEVTSMASKLSPVGAEVDDQS
                     MTLVRFADGKVGYIGSCWTSPGIFACRVFGSTGLMHYEIDFGTWDTPDRLHDASTLYI
                     QRGKDGWAKREMLKVNASDMFRAELEMFADTVRSGKAGELSADNGNVALAVVYAALRS
                     IDQKGQYVRIADVINDARARLRQAA"
     gene            1755..>2379
                     /locus_tag="AC16CX_06760"
     CDS             1755..>2379
                     /locus_tag="AC16CX_06760"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3012252.1"
                     /translation="MISSRYFWELTQPEIAAQLKKNPLVILPAGSVEQHGPHLPAGTD
                     IFAANVIAHAVAERMDGLVLPGGPLGVTPMHMPFEATITLTPGTFQRVVTETCVSTAR
                     HGAKYLLILNWHEGNIPSLAIAAEQLHRDHGMTVLTVQACYVAAEMFGNEFGGLTHGG
                     EIEVLAVLACRPELVHLDRIDDSSDHTHGRKWDKLRRTRSYQPVLRDI"
BASE COUNT          441 a          787 c          751 g          400 t
ORIGIN      
        1 accaccttgc gaagcggccg gacgtggaca tgagccgcat cgttgtccag ggccgcagct
       61 ggagcggcta ctgggcggcc aagctcgcga tcaccgagcg cagccgcctg cgaggggtgg
      121 tggtgcacgg cgggccgatc caccattact tccagcccga ctggctcaag ccttccctcg
      181 cgaccggcga atacctctac gattatctcc aggccaaatg cgcgatgatg ggcgcagaag
      241 gtgtggatga gttgctcgac cgtggacgcg gtttctcact gctcgaggcg ggcctgctcg
      301 accggccttc cgcgccgatg ctggtggtca acggcgcgcg cgatacgcag gttcccgtcg
      361 cggacgcata cgtgctcctg gaacatggcg aggccaagga ggcctggatc aatccccaag
      421 gcgggcacat gggccgctcg gcgcagtggc caagttccgc gatcgcggag aaagtattga
      481 tgccgtggat cacgcgccgg cttggtcaag cgttgtagaa ttacgcacca atctccataa
      541 caagggattt ccatgggcag cactcccgtc aaactgggta tcgtcggcct cggccgctgg
      601 gccaaggtgc tgacccgcgc gtccaggcag tccgacaagc tgcggatcgt cgccggctac
      661 agccgctccg aggacaagcg ccggtcgttc cagcaggagt tcggaatccc ggtgacgccc
      721 gatctgaaga cactgctcgc ggagcccgag atcaaggggg tcatactcac cgtgcccaac
      781 gagcagcacc tgccggtgtc ggaggaagta gcgagggcga agaaacacat ctacaccgag
      841 aagccgattg cgaacacgct ggaagacggt ttgaggatcg ccgcgctcga gaagcagtac
      901 ggcgtcaccg tcaccgtcgg gcacagcgcg cgcctgatgg ccggtttccg ccagatcagg
      961 gctgcgatcg acgcgggcga gctgggacgt gtcggcttca tcgaggccaa cttctcgaac
     1021 gagcgcgcgc tcgagttgac gcccagcacc tggcgctggt accggtccaa ggcgccaggg
     1081 gggccgctct cgcagctcgc gatccaccag ttcgacgtcc tgcactacct cgggggggaa
     1141 atggtggagg tcacctccat ggcctccaag ctctcgccgg tcggcgcgga agtggacgac
     1201 caatccatga cgctggtacg cttcgccgac ggtaaggtgg gctatatcgg ctcgtgctgg
     1261 acctcgcccg gcatctttgc ctgccgcgta ttcgggtcga cgggattgat gcactacgaa
     1321 attgatttcg ggacgtggga cacgcccgac aggctgcacg atgcatccac cctctacatc
     1381 cagcgcggca aggacggttg ggcgaagcgc gagatgctca aggtcaacgc gagcgacatg
     1441 ttccgcgccg agctggaaat gttcgcggac accgtccgca gcggcaaagc aggggagctt
     1501 tcggcggaca acggcaacgt cgcgctggcg gtggtctacg ccgcactgcg ctcgattgac
     1561 cagaagggcc agtacgtgcg catcgccgat gtgatcaacg acgcgcgcgc caggctgcgc
     1621 caggcagcgt agcggccgtg gctggtgact cgtgactcgt gtctggtgag tggattacag
     1681 cttttccctt ccattgctca tagatgatct gactctttcc actcactact caccattcac
     1741 tactcaccga ctttatgatt tcgagccgct acttctggga gctgacgcag ccggaaatcg
     1801 cggcgcagct caagaagaac ccgctcgtga tcctgccggc gggcagcgtc gagcagcacg
     1861 gcccgcacct gccggcgggc accgacatct tcgccgccaa cgtcatcgcg cacgcggtgg
     1921 cggaacgcat ggacggcctc gtgctcccgg gcggtccgct tggcgtcacc cccatgcaca
     1981 tgcccttcga ggccaccatc accctcaccc ccggcacctt ccagcgcgtg gtgaccgaaa
     2041 cctgcgtgtc caccgccagg cacggggcca aatacctgct gatcctgaac tggcacgagg
     2101 gcaacatccc ctcgctcgcc atcgccgccg agcaactcca ccgcgaccac ggcatgaccg
     2161 tattgacggt gcaggcgtgc tatgtcgccg ccgagatgtt cggcaatgaa ttcggcggcc
     2221 tgacccacgg cggcgagatc gaggttctcg cagtgctcgc ctgccgcccc gaactcgtcc
     2281 acctcgatcg catcgatgac tcctcagacc atacgcacgg ccgcaagtgg gacaagctgc
     2341 gccgcacccg gtcctatcag ccggtgctgc gcgacatcc
//
LOCUS       DBNGWZ010000226         5348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1785820, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000226 DBNGWZ010000000
VERSION     DBNGWZ010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1785820"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC16CX_06765"
     CDS             <1..676
                     /locus_tag="AC16CX_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3012253.1"
                     /translation="LAAPIPRPRKNVFCVGWNYLDHFEEGAKSRTQKVDLPEHPAFFS
                     KAATAVTGPYDAIPFDAAASEKIDWEVELGVIIGPGGRNIPEAAAMKHVFGYAVINDV
                     SARDLQRRHNQWFKGKSLDGSCPMGPWIVTADEVPDPNNLRLTTRVNGAVKQDSNTRY
                     FYFKLPRIIAELSLGMTLEAGDIIATGTPSGVGYARTPPEFLKPGDVLETEVVGVGLL
                     RNRVGS"
     gene            complement(970..1575)
                     /gene="metW"
                     /locus_tag="AC16CX_06770"
     CDS             complement(970..1575)
                     /gene="metW"
                     /locus_tag="AC16CX_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR3012254.1"
                     /translation="MRNGNGTITQRPDFAAIAGWVAMGSSVLDLGCGDGSLLCYLRES
                     RRVTGYGIEIEDDGVLDCVKNGVNVIQGDLERGLSDFESRSFDYVILSLTLQAVRHSE
                     RIIAEMLRVGREGIVTFPNFGYWKNRLQVIGGHMPVSDNLPYEWHDTPNVHLCTIADF
                     ERFCAGRAVKILERKVLTRGRPVALLPNLLGSLAVYRFQRA"
     gene            complement(1572..2720)
                     /gene="metX"
                     /locus_tag="AC16CX_06775"
     CDS             complement(1572..2720)
                     /gene="metX"
                     /locus_tag="AC16CX_06775"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase MetX"
                     /protein_id="HIR3012255.1"
                     /translation="MDIGVTPQSVGAVTPQTARFNEPIRLRGGAVLDAYELVYETYGR
                     LNADRSNAVLVCHALNAAHHVAGYYADDPENVGWWDNMIGPGKPVDTNRFFVVGVNNL
                     GGCHGSTGPMSINPKTGKPWGADFPVVTVEDWVATQARLADHLGIRRFAAVIGGSLGA
                     MQALAWAYSHPGRVRHSIVIACAPNLSAQNIAFNEVARQAIVTDPDFHGGHYYEHNTR
                     PTRGLRVARMIGHITYLSDDEMASKFGRQLRNGKLGYSFDTEFEIESYLRHQGLKFSD
                     YFDANTYLRITKVLDYFDPALEHGGSLARALAPAKANFLVVSFTTDWRFSPQRSREIV
                     KALLDNRLDVSYAEIDAPHGHDAFLLEDARYHRLIAAYFDRIGKEAAA"
     regulatory      complement(2770..2847)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(3024..4751)
                     /gene="ptsP"
                     /locus_tag="AC16CX_06780"
     CDS             complement(3024..4751)
                     /gene="ptsP"
                     /locus_tag="AC16CX_06780"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR3012256.1"
                     /translation="MSFTIHGIGVAGGIAIGHAQLMTHAGLEVPRYVVPPHEVPEESA
                     RFDAAVKQVRSEFEELRASVPPNAPAEFGAFINLHQMILDDSTLSVAPRRIIETEGCN
                     AEWALRVQTDTLLARFDEMEDEYLRERKSDVIQVVERVMKALSGQPGYVPPPPAGRDQ
                     SLILVAHDLSPADVVQFKRHQFTSFITDLGGTTSHTAVVARSLAIPSIVALHRARQLI
                     REDELIIVDGTQGVVIVDPDEQVLAEYQLRQHQFDLERQKLKRLKDRRAVTMDGVPVE
                     LQANIELPADVAEAKDNGATGIGLFRSEFLFLNRSDLPDEDEQFEAYRKVAAEMDGMP
                     VTIRTYDLGADKDDVEERVTTNPALGLRAIRLCLTEPRRFITQLRALLRASHYGKVNI
                     LIPMLATASEIDQTLALIQLAKQSLDEESVPYDRGIQVGGMIEVPAAAIALGMFIRKL
                     DFLSIGTNDLIQYTLAIDRTDDTVAHLYDPLHPAVLNLLAGIIRTADKAKVPVALCGE
                     MAGDTLLTRLLLAFGLRQYSMHTAHLLDVKQIILKSNCSQLKPLARKMLRTTDAAKLH
                     ALLEKLNSR"
     gene            complement(4856..5125)
                     /locus_tag="AC16CX_06785"
     CDS             complement(4856..5125)
                     /locus_tag="AC16CX_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR3012257.1"
                     /translation="MLQQEVEIINKLGLHARAAAKLTQVAGRFKANVWIGGNGRRVNA
                     KSIMGVMMLAAARGSKVLVETDGPDEEEAMDAVTRLIAGRFEENE"
     gene            complement(5106..>5348)
                     /locus_tag="AC16CX_06790"
     CDS             complement(5106..>5348)
                     /locus_tag="AC16CX_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3012258.1"
                     /translation="GVLILSDMYGGSPSNIAAKLLVPGKVEGVAGVNLPMLIRVLTYR
                     DKPLATVVIKAVSGGCDGVVRIPAPALVVNNAATGS"
BASE COUNT          896 a         1685 c         1820 g          947 t
ORIGIN      
        1 gctcgcggcg ccgatcccgc gcccgcgcaa gaacgtgttc tgcgtcggct ggaactacct
       61 cgatcacttc gaggaggggg cgaaaagccg cacccagaaa gtggacctgc ccgagcaccc
      121 cgctttcttc tccaaggcgg cgaccgcggt caccggcccc tacgacgcga tcccgtttga
      181 tgcggcggcc tcggaaaaga tcgactggga ggtggagctc ggcgtcatca tcgggccagg
      241 ggggcgaaac attcccgaag ccgcggcgat gaagcacgtc ttcggctatg ccgtgatcaa
      301 cgacgtctcg gcgcgcgacc tgcagcgcag gcacaaccag tggttcaagg gcaagagcct
      361 ggacgggtcg tgcccgatgg gtccctggat cgtcaccgct gacgaagtgc ccgaccccaa
      421 caatctgcgc ctgaccacgc gcgtcaacgg cgccgtcaag caggactcga acacgaggta
      481 tttctacttc aagctgccgc gcatcatcgc ggagctctcg ctcgggatga cgctggaggc
      541 gggtgacatc atcgcgaccg gcacgccctc cggcgtgggc tacgcgcgca cgccgccgga
      601 gttcctgaag ccgggcgacg tgctggagac cgaggtcgtg ggcgtggggc tgctgcgcaa
      661 ccgggtcgga agctgaccgc cgaagagatc aagatcaagc acgaaccgcc aaggcgccaa
      721 gagaagcaaa aataataatc gaaccaccga gacgcagaga gaaacgaggg cgagaattga
      781 cggcaggaaa cggaagggtt attcaacaac acgggcgcga aatcgcgccc gttttcgttt
      841 tcccccatct tccgccataa ggactttgtc cagattctgg atttggtttt cttggcgtct
      901 tggcggttca atcagtggtc ttgtttttcc tgtgtgtcct ttgtgtcctt ggtggttccg
      961 ctttagtctt tacgcgcgct ggaagcggta caccgcgagg ctgccgagca ggttcggcag
     1021 cagcgcgacc ggccggccgc gcgtgagcac cttgcgctcg aggatcttca ccgcgcggcc
     1081 cgcgcagaag cgctcgaagt cggcgatcgt gcacaggtgc acgttcggcg tgtcgtgcca
     1141 ctcgtacggc aggttgtccg acaccggcat gtggccgccg atcacctgca gccggttctt
     1201 ccagtagccg aagttgggaa aggtgacgat gccctcgcgc ccgacgcgca gcatttcggc
     1261 gatgatgcgc tcgctgtgcc tcaccgcctg cagcgtcagc gacaggatca cgtagtcgaa
     1321 cgagcggctc tcgaaatcgg acagcccgcg ttccagatca ccctggatca cgttcacgcc
     1381 gttcttcacg cagtcgagca cgccgtcgtc ctcgatctcg atgccgtagc cggtgacgcg
     1441 gcggctttcc ctcaggtagc acaggagact gccgtcgccg cagcccaggt cgagcacgct
     1501 cgagcccatg gccacccagc ccgcgatcgc cgcgaagtcc ggccgctgcg tgatggtgcc
     1561 gttgccgttt ctcatgcggc cgcctctttc ccgatccggt cgaagtaggc ggcgatcagc
     1621 cggtggtagc gcgcgtcctc cagcaggaac gcgtcgtgcc cgtggggcgc gtcgatctcg
     1681 gcataggaga cgtcgaggcg gttgtcgagc agcgccttga cgatctcgcg cgagcgctgg
     1741 ggcgagaagc gccagtcggt cgtgaacgaa accacgagga aattcgcctt cgcgggcgcg
     1801 agcgcccggg cgaggctgcc gccgtgctcg agcgccgggt cgaagtagtc gagcaccttg
     1861 gtgatgcgga gataggtgtt ggcgtcgaag taatcggaga acttcaggcc ctggtggcgc
     1921 aggtacgact cgatctcgaa ctcggtgtcg aacgagtagc cgagttttcc gttcctcagc
     1981 tgccggccga acttgctcgc catctcgtcg tccgagagat aggtgatgtg gccgatcatg
     2041 cgcgcgacgc gcaagccccg cgtcggccgc gtgttgtgct cgtagtaatg gccgccgtga
     2101 aaatcggggt cggtgacgat cgcctggcgc gcgacctcgt tgaacgcgat gttctgcgcc
     2161 gagagattgg gcgcgcaggc gatcacgatc gagtggcgca cgcgcccggg atggctgtag
     2221 gcccaggcga gcgcctgcat cgcgccgagg ctgccgccga tcacggcggc gaagcggcgg
     2281 atgccgaggt ggtcggcgag ccgggcctgg gtcgcgaccc aatcctccac cgtcaccacc
     2341 ggaaaatcgg cgccccacgg cttgccggtc ttcgggttga tgctcatggg cccggtcgag
     2401 ccatggcagc cgccgaggtt gttgacgccg acgacgaaaa agcgattggt gtccaccggc
     2461 ttgcccgggc cgatcatgtt gtcccaccag ccgacgttct ccggatcgtc ggcgtaatag
     2521 ccggcgacgt ggtgggcggc gttcaacgcg tggcacacca gcactgcgtt ggagcggtcg
     2581 gcgttgagcc tgccgtaggt ctcgtaaacg agctcgtacg catccagcac cgcgccgccc
     2641 ctgagccgga tcggctcgtt gaagcgggcg gtctgcggcg tgacggcgcc gaccgattgc
     2701 ggcgtgaccc caatgtccat aagaaacggc aaataaaaac ccggcgggct tgcgctggcc
     2761 gggttcgaaa aaaccttgaa tgttttctct ttagccggat ttgtaacgcg cccgcaagct
     2821 gaaaatcaaa tcggcgcggc ctgaagcggg aggaacatta cccgctcggg ggaacgatgt
     2881 caaatgaggg ctttcgaggt gactgcggtc caatttgagg aatgaggaat gagggatgag
     2941 ggatgaggca attcccgcgc gttgagcggc gtgatttcgt ccgctcgaac gcgcggagct
     3001 gaaccggcgc gtagcgtccg gattcagcgg ctgttgagtt tttcgagcag cgcgtgcagc
     3061 ttcgcggcgt cggtggtgcg cagcatcttc cgcgcgagcg gcttcagctg cgaacagttc
     3121 gacttgagga tgatctgctt cacgtcgagc aggtgcgcgg tgtgcatcga gtactgccgc
     3181 agcccgaacg cgagcagcag gcgcgtaagc agggtgtcgc cggccatctc gccgcacagg
     3241 gcgaccggca ccttcgcctt gtccgcggtc cggatgatcc ctgcgaggag gttgagcacc
     3301 gccggatgca ggggatcgta gagatgcgcc accgtgtcgt cggtgcggtc gatcgccagc
     3361 gtgtactgga tgaggtcgtt ggtgccgatc gagaggaagt cgagcttgcg gatgaacatg
     3421 ccgagcgcga tcgccgccgc cggcacctcg atcatgccgc ccacctggat gccgcggtcg
     3481 tagggcacgc tttcctcgtc caggctctgc ttggcgagct gaatcagcgc aagcgtctgg
     3541 tcgatctcgg aggcggtggc gagcatcggg atcaggatgt tcaccttgcc gtagtgggag
     3601 gcgcgcagca gggcgcgcag ctgggtgatg aagcgccgcg gctcggtgag gcacagccgt
     3661 atcgcgcgca gccccagcgc cgggttggtg gtgacgcgct cctcgacgtc gtccttgtcg
     3721 gcgccgaggt cgtaggtgcg gatggtgacc ggcatgccgt ccatctccgc ggcgaccttg
     3781 cgataggcct cgaactgctc gtcctcgtcc ggaaggtccg atcggttgag gaacaggaac
     3841 tcgctgcgga agaggccgat gccggtcgcg ccgttatcct tggcctccgc gacgtccgct
     3901 ggcagctcga tgttcgcctg cagctcgacc ggcacgccgt ccatcgtgac cgcgcgccgg
     3961 tccttcaggc gcttcagctt ctgccgctcg aggtcgaact ggtgctgccg cagctggtac
     4021 tcggcgagca cctgctcgtc ggggtccacg atcaccacgc cctgggtgcc gtccacgatg
     4081 atgagctcgt cctcgcgaat cagctgccgc gcgcggtgca acgccacgat cgaggggatg
     4141 gcgaggctgc gcgcgaccac cgcggtgtgc gaggtagtgc cgccgagatc ggtgatgaag
     4201 ctcgtgaact ggtggcgctt gaactgcacc acgtcggcgg gcgagagatc gtgcgcgacg
     4261 aggatcaggc tctggtcgcg gccggcgggc ggcggcggca cgtagccggg ctggccggag
     4321 agcgccttca tcacgcgttc gacgacctgg atcacgtcgg acttgcgctc gcgcagatac
     4381 tcgtcctcca tctcgtcgaa ccgggcgagc agcgtgtcgg tctgcaccct gagcgcccac
     4441 tcggcgttgc agccctcggt ttcgatgatc cggcgcggcg cgaccgacag ggtggagtcg
     4501 tcgaggatca tctggtgcag gttgatgaag gcgccgaatt ccgcgggagc gttgggcgga
     4561 acgctggcgc gcagctcctc gaactccgaa cgaacctgct tgaccgctgc gtcgaagcgg
     4621 gcggattcct ccggcacctc gtgcggcggc acgacgtagc gcggcacctc cagccccgcg
     4681 tgcgtcatca gctgcgcatg cccgatggcg atgccgccgg cgacaccgat gccgtgtatc
     4741 gtgaaactca tacgctccgc gagtgaggcg agagggttga ggagagagga gtagtttatc
     4801 cgcggcggac gccgcgcgcg aggctgttac cccccacttc ttactcctca cggcgttatt
     4861 cgttttcctc gaaccggccg gcgatgagcc gcgtcaccgc gtccatcgcc tcctcctcgt
     4921 cgggcccgtc ggtctcgacc agcaccttcg agcccctggc cgccgccagc atcatcaccc
     4981 ccatgatgct cttggcgttg acgcggcggc cgttgccgcc tatccagacg ttggctttga
     5041 agcggccggc gacctgagtc agcttggccg ctgcccgggc gtgcagaccg agcttgttga
     5101 tgatctcaac ttcctgttgc agcattgtta acgacgagcg cgggggcggg gatgcgcacc
     5161 acgccgtcgc acccgccgct caccgccttg atgacgacgg tggcgagcgg cttgtcgcga
     5221 taggtcagga cccggatcag catcggcagg ttgacgccgg ccacgccttc aaccttgccg
     5281 ggcacgagca acttggcggc gatgttggaa ggcgagccgc cgtacatgtc cgagaggatc
     5341 agcacgcc
//
LOCUS       DBNGWZ010000227         2372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1533939, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000227 DBNGWZ010000000
VERSION     DBNGWZ010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1533939"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16CX_06795"
     CDS             <1..399
                     /locus_tag="AC16CX_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR3012259.1"
                     /translation="MAEKPTKPYLIRAIHEWCTDSGLTPYLSVQVNAETRVPAEQVKN
                     GEIVLNVSYDATHRLTIGNDVIQFAARFNGVSRECSIPIDAVTGVFAKENGQGLFFPR
                     EAAPESGGKSPSEPSPPNGNGGKPRLQVIK"
     gene            complement(502..1395)
                     /locus_tag="AC16CX_06800"
     CDS             complement(502..1395)
                     /locus_tag="AC16CX_06800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012260.1"
                     /translation="MTKERRPVGMIGIGLMGTACAKRLLAAGFDVLGYDVDADKLKPF
                     TAQGGRAARSVTEIARERDRVVLAVFNTDQVEDAIEGPHGLLAARPADAPPLTAVCVS
                     TCDPDRIAALAARLPAARLRFIEAPVSGTSDQTARGEALGLIGGDRAVVDAARDVIDA
                     ICPRWHYLGAAGNGGRAKLAINLILGINRAALAEGLVFAERMGLAPEKFLEVARESAA
                     YSQIMDVKGRKMLSGDFSPQGYVTQSLKDFSLMLEQAKRVGQRLSLGEAYAGLMEGCV
                     AAGEGDLDNSAVIREIRRRTG"
     gene            1562..1636
                     /locus_tag="AC16CX_06805"
     tRNA            1562..1636
                     /locus_tag="AC16CX_06805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1594..1596,aa:Thr,seq:tgt)
     gene            1879..2097
                     /locus_tag="AC16CX_06810"
                     /pseudo
     CDS             1879..2097
                     /locus_tag="AC16CX_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2250..>2372
                     /locus_tag="AC16CX_06815"
     CDS             2250..>2372
                     /locus_tag="AC16CX_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3012261.1"
                     /translation="MLYQSIDTLSKGYKHRTCLAQALIHDPDVLIMDEPTDGLDP"
BASE COUNT          457 a          781 c          747 g          387 t
ORIGIN      
        1 atggcggaaa agcccaccaa accgtacctc atccgggcga tccacgagtg gtgcacggac
       61 agcgggctga cgccgtacct ctccgtgcag gtgaacgccg agacccgggt tccggcggag
      121 caagtgaaga acggcgagat cgtgctcaac gtgagttacg acgccactca ccggctcacc
      181 atcggcaacg atgtcatcca gttcgcggcg cgcttcaacg gggtctcgcg cgagtgctcg
      241 atcccgatcg acgccgtgac cggcgtcttc gccaaggaaa acgggcaggg cttgttcttc
      301 ccgcgcgagg cggcgccgga aagcggcggc aaatcaccct cggagccgtc cccgccgaat
      361 ggcaacggcg gcaagcccag gctgcaggtc attaagtaag ctgtcaccag tcaccagtca
      421 cgagtcacga gtcacgagtc acgagtcacg agtcacgagt cacgagtcac gagtcacgag
      481 tcacgagtca cgagtcacgg ttcatccggt tcggcggcgg atctccctga tcaccgcgct
      541 attgtcaaga tcaccctcgc ccgcggcgac acagccctcc atgagaccgg cataggcctc
      601 gcccaacgac agtcgctgtc caacacgctt cgcctgctcg agcatgagcg agaaatcctt
      661 gagcgactgg gtgacgtatc cctggggtga aaaatccccc gacagcatct tgcggccctt
      721 gacgtccatg atctgcgaat aagcggccga ttcgcgcgcg acctcaagaa atttttccgg
      781 ggccagtccc atgcgctcgg cgaacaccaa cccttcggcg agcgccgcgc ggttgatgcc
      841 gaggataaga ttgatcgcga gctttgcacg tcctccgttg ccggcggccc cgagatagtg
      901 ccagcgcgga cagatcgcgt cgatcacgtc gcgcgccgca tccaccacgg cccggtcgcc
      961 gccgatgaga cccagcgcct cgccgcgcgc ggtctggtcg ctggtgccgg aaacgggcgc
     1021 ctcgatgaag cgcagacggg cggcgggcag gcgcgcggcg agcgccgcga tgcggtcggg
     1081 gtcgcaggtg ctcacgcaga ccgcggtaag cggcggcgcg tccgccggcc gcgcggcgag
     1141 cagtccatgc gggccctcga tcgcgtcttc cacctgatcc gtgttgaaaa ccgcgagcac
     1201 gaccctgtcg cgctcgcgcg cgatctcggt gaccgagcgc gcggcgcgcc cgccctgtgc
     1261 cgtaaacggc ttgagcttgt ccgcgtcaac gtcgtatccc agcacgtcga agccggccgc
     1321 gagcaggcgt ttcgcgcagg ccgttcccat cagcccaatg ccgatcatcc cgaccggccg
     1381 tcgttccttc gtcatgcaac ctccctcagt cctgcaagtt cccgcaaatt ataggcgcca
     1441 gcgctgctgg aaacgggaag gcagaaggca gaaggcagaa ggcagaaggc acactacctg
     1501 acagttttcc tacacttcat ccttctgcct tcatccttca tcccttataa ttccgtccca
     1561 ggccggcata gctcagtggt agagcaaccg ccttgtaagc ggtaggtcgt ctgttcgagt
     1621 cagactgtcg gcaccagcac ggcacggacc tgcggcgcgg cgggcctgtt gtaggaagga
     1681 tgcaggtcct gcaacgaatt cggatggaaa acaagccgtt aattgacggc cacgggaacc
     1741 tttgcaattt ttccggtcaa acaggcgcga acaccggcac gtcgccggtg gttgaaatct
     1801 gcggctccgg gcacagctga tgggacagga aaaaaggcgg cggccgcaac ggccggttac
     1861 cgccgctgag gaaagggcat gatcaaagta gacaatctgg tgaaagcttt cggcgcgaag
     1921 ctcgcggtga acgacgtctc gttcacggtc gagcgcggcg aagtgctggg cttcctgggg
     1981 cccaacggcg cgggcaaatc caccaccatg cgcatgttga cgggattcct cccgccgacc
     2041 tcggggaaga tcaccgtcgg cggctacgac gtgctggagg acccgctgcc ggcgaagcgc
     2101 ctgatcggct acctgcccga gaacgcgccg ggctacgccg acatgacggt gcatggtttc
     2161 ctcaactttg ccgccgagct gcgcggcctg cgggcgaggc gcggaagcag gcggtcaacc
     2221 gcgtggtcga gctgtgcttt ctggggagcg tgctgtacca gagcatcgac acgctgtcca
     2281 agggctacaa gcaccgcacc tgtctcgcgc aggcgctgat ccacgatccc gatgtcctga
     2341 tcatggacga gccgaccgac gggctcgatc cc
//
LOCUS       DBNGWZ010000228         2608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1534310, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000228 DBNGWZ010000000
VERSION     DBNGWZ010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1534310"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="AC16CX_06820"
     CDS             complement(<1..1526)
                     /locus_tag="AC16CX_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3012262.1"
                     /translation="MDKPFAITLDVGSSLANLTGSWRTNRPVYVDRLPPCNDACPAGE
                     NIQGWLYYAESGDYEAAWRTLTLDNPLPAVMGRVCYLPCESACNRAHLDEAVGINSVE
                     RFLGDEAIRRGWRFDPPVHDSGKRVLIVGAGPSGLSAAYHLRRLGHRVTIHEAGPFAG
                     GMMRFGIPKYRLPREVLDAEVNRILAMGVKLRLDSKVSNVLEVMRKGRYDAVFLAVGA
                     HIGKRAYLPAGSAAKVLDAVSVLRSMEGQEKPLLGRRVVVYGGGNTAIDVARTARRLG
                     ATESIIVYRRTREKMPAHDSEVEEALLEGIMIKWLSTIKEAGEASITVEKMTLDENGF
                     PQPTGELETLEADSVVLALGQDVDLSLLKGVPGLEINDGVVQVDPNMMTGYAGIFAGG
                     DMVPAERTVTVAIGHGKKAARRIDAWLRRAQYLPPEKHELATVDRLNTWYYSDAPKTM
                     RPTLDIIRRQSTFDEVVGSLDESNALYEARRCLSCGNCFECDNCYGVCPDNAVIKLGP
                     G"
     gene            complement(1526..2527)
                     /locus_tag="AC16CX_06825"
     CDS             complement(1526..2527)
                     /locus_tag="AC16CX_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012263.1"
                     /translation="MSFQPIQFYQTGTFTVGNRLLAPDERSVQANMQRTNTLNSGHRA
                     CQGCGEALGARYAIDAAMNATANQLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAALKVKGRHDVRVIAQGGDGGTTDIGFGCLSGMFERNDDVLFICYDNEAY
                     MNTGVQRSSATPPAARTTTTLPVGPQPGNVFGQGKNVALIAMAHEIPYVATATVAELR
                     DLEAKVRRAMEIRGARYIHIHVPCPLGWGHEACDTIRIARLAKETGLFPVFEAERGEI
                     VSVSKIRRKLPVEEYLRPQRRYAHLFGENPRADIIARVQALADRNIRKFGLLEEGVH"
BASE COUNT          379 a          866 c          909 g          454 t
ORIGIN      
        1 ccggggccga gcttgatcac ggcgttgtcg gggcacacgc cgtagcagtt gtcgcactcg
       61 aagcagttgc cgcacgagag gcagcgccgc gcctcgtaga gcgcgttcga ctcgtcgagg
      121 ctgcccacca cctcgtcgaa agtggattgc cgccggatga tgtcgagcgt cgggcgcatc
      181 gttttcggcg cgtccgagta gtaccaggtg ttgagcctgt cgaccgtggc gagctcgtgc
      241 ttctcgggcg ggaggtactg cgcccgccgc agccacgcgt cgatgcggcg cgcggccttc
      301 ttgccgtgcc cgatcgcgac ggtgacggtg cgctccgcgg gcaccatgtc gccgcccgcg
      361 aaaatgccgg catagccggt catcatgttc ggatcgacct gcaccacgcc gtcgttgatc
      421 tccagcccgg gcacgccctt cagcaggctc aagtccacgt cctgccccag cgccagcacc
      481 accgagtcgg cttccagcgt ctcgagttcg ccggtgggct gcgggaaacc gttctcgtcg
      541 agcgtcattt tttccaccgt gatgctggcc tcgccggctt ccttgatggt ggacagccac
      601 ttgatcatga tgccttccag cagcgcttcc tcgacttcgg agtcgtgcgc cggcatcttc
      661 tcgcgggtgc ggcggtagac gatgatggac tccgtggcgc cgagccgcct ggcggtgcgc
      721 gcgacgtcga tggcggtatt gccgccgccg tagaccacga cgcgccgccc gagcaagggc
      781 ttctcctgcc cttccatgct gcgcagcacg gataccgcgt cgagaacttt cgccgcggat
      841 cccgccggga ggtaggcgcg cttgccgatg tgcgcgccca ccgcgaggaa cacggcgtcg
      901 taacggcctt tccgcatgac ttcaaggacg ttgctcacct tcgagtcgag cctgagcttc
      961 acgcccatgg cgaggatgcg gttcacctcg gcgtccagca cctcgcgcgg cagccggtac
     1021 ttgggaatcc cgaaccgcat catgccgccg gcgaacggcc ccgcttcgtg gatcgtgacg
     1081 cgatggccca ggcggcgcag gtgataggcg gcggaaaggc cggaggggcc ggcgccgacg
     1141 atcagcacgc gcttgccgga atcatggacc ggcggatcga atcgccagcc gcgccggatg
     1201 gcctcgtcgc cgaggaaacg ctccaccgag ttgatgccca ccgcctcgtc gagatgggcg
     1261 cggttgcagg cgctttcgca cggcaggtag cagacgcgcc ccatcaccgc gggcaggggg
     1321 ttgtcgagcg tcagcgtgcg ccaggcggcc tcgtagtcgc cggattcggc atagtagagc
     1381 cagccctgga tgttctcgcc cgcggggcac gcgtcgttgc acggcggcag gcggtcgacg
     1441 tagaccgggc ggttggtgcg ccacgatccg gtgaggttgg cgagcgagga gccgacgtcg
     1501 agcgtgatgg cgaacggctt gtccatcaat gcactccttc ctcgagcagg ccgaacttgc
     1561 ggatgttgcg gtccgcgagc gcctgcacgc gcgcgatgat gtcggcgcgc ggattctcgc
     1621 cgaagagatg cgcgtatcgc cgctgcggcc gcaggtactc ctccaccggc agcttgcggc
     1681 ggatcttcga tacgctcacg atctcgccgc gctcggcctc gaacacgggg aacagccccg
     1741 tttccttggc gagccgcgcg atgcggatcg tgtcgcaggc ctcgtggccc cagccgagcg
     1801 ggcagggcac gtggatgtgg atgtagcgcg cgccccggat ctccattgcg cgcctgactt
     1861 tggcctcgag atcgcgcagc tccgcgaccg tggccgtcgc gacgtaggga atttcgtggg
     1921 ccatggcgat cagcgcgacg ttcttgccct gcccgaacac gttgcccggc tgcggaccca
     1981 ccggcagggt ggtggtggtg cgcgccgcgg gcggcgtggc cgaggagcgc tgcacgccgg
     2041 tgttcatgta ggcctcgttg tcgtagcaga tgaagagcac gtcgtcattg cgctcgaaca
     2101 tccccgagag gcagccgaag ccgatgtcgg tggtgccgcc gtccccgccc tgcgcaatca
     2161 cccttacgtc gtggcggccc ttgaccttca gcgccgctgc gatcccggtt gcgaccgcgg
     2221 cggtattgcc gaacaggctg tggatccagg ggatctgcca cgacgtctcg ggatagggcg
     2281 tggagaagac ctccaggcag ccggtggcat tggctgcgat cagctggttc gcggtcgcgt
     2341 tcatcgccgc gtcgatcgca tagcgtgcgc cgagcgcctc tccgcaaccc tggcacgcgc
     2401 ggtggccgga gttgagcgta ttggtgcgct gcatgttggc ctgcaccgag cgctcgtccg
     2461 gggcaagcag ccggttgccg accgtaaaag tgccggtctg gtagaactgg atgggctgga
     2521 acgacatggc gtcctctcaa ctcggtcgcg ccgcgaccac gccgacgtcg cgcaggatgg
     2581 cttcggctat ggggccggag cggcgttg
//
LOCUS       DBNGWZ010000229        10126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1282832, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000229 DBNGWZ010000000
VERSION     DBNGWZ010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1282832"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC16CX_06830"
     CDS             complement(<1..872)
                     /locus_tag="AC16CX_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3012264.1"
                     /translation="MTKPPQVIVAGAGPVGLLTALALARQEVSVLVFEAEPGLTVDLR
                     AGTYHPPSLEMMAPYGITDEMLKTAIKVPRWQIRDRKEGVIVDWDVSLIKDLTPYPYR
                     LHLEQHRLTPIIYSKLGECPNAEVRFSHEVTGFAQTADRVVVTVKTPEGMERVEAAWL
                     VGADGGRSTVRRIMGVGFEGYTWPERFLVASTNYDYEPHGFRLNTYVADPVDWNAMFK
                     MPGDGPPGLWRVLFPVRPEEDEAAALSEDNVERLMQGFQPKAGRYAIQYKSIYKVHQR
                     VASDFRLGRALLAG"
     gene            complement(892..2145)
                     /locus_tag="AC16CX_06835"
     CDS             complement(892..2145)
                     /locus_tag="AC16CX_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012265.1"
                     /translation="MTDPAVNVAAERAAPPSFAALRHGGFRIYFVTTAAAMMADNIEH
                     VISYWVMFEKFHSPALAGFAVLSHWLPFLLFSVYFGAMADRFDSRRIIQVAMFMFMVV
                     SLAWAVLFYTDTIEVWHAAVLLVVHGMAGVLWAPASQVLIHDIVGRENLVSAVRLNAT
                     GRQLGVFLGPGVGGGLMLLLGPAAGLLVNVLIYLPLAWWLWKAPYGADRRRESVLARP
                     GGFVDAMKTLQAVSGNHTILSMILLAGLSSLFVGNAFLAQMPEYAQDLGTEKPDFSYA
                     VLLGSNAAGAFIGGMILESRGLLRSTPHTAIVLTILWCFSIAGFAATASYPLAVALMF
                     VAGFLSLAYSSMAQALVQLEAPAHLRGRLIGLFQMSNNGLRAFSGITVGILGSLIGIH
                     WSLALSALALLAVTATLLAFSMRTR"
     gene            complement(2202..2681)
                     /locus_tag="AC16CX_06840"
     CDS             complement(2202..2681)
                     /locus_tag="AC16CX_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3012266.1"
                     /translation="MLKSVVRTMILLSIALFCAPAAAQAFEKQHFHHNEWTKGRFSEA
                     VTVTGPGKWVFLAGVGAEAEKDGAILHIGDFTAQCRYAYDKIRKLLAAHGATMADVIK
                     LVTYVVDIRDRDAMNRCRAEAFKGGPLPVHTFLAISQLARPGMLVEMDVIAVVPAGK"
     gene            complement(2784..3296)
                     /locus_tag="AC16CX_06845"
     CDS             complement(2784..3296)
                     /locus_tag="AC16CX_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR3012267.1"
                     /translation="MITTRTARMLARYNAWANQLIFDAVAALPEGEATKERRSLFKNM
                     VHTLNHNYVIDRIWQAHLEGREHGYEARNTPDHPPLADLWRAQQEIDRWYIAWSDRLS
                     DAALDEKVSFTLIGGNRGVMTRGEILLHVVNHTSYHRGFVADLFYQVPARPPTTDLPV
                     FLRETASVDR"
     gene            3574..4326
                     /locus_tag="AC16CX_06850"
     CDS             3574..4326
                     /locus_tag="AC16CX_06850"
                     /inference="COORDINATES: protein motif:HMM:NF036686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR3012268.1"
                     /translation="MFAWHKRIGHICPGAISTSAYDFYLVAPEGVGLFCVTNPIEDWR
                     DDEYEQSLARVDEAANYLASVHADFIAHVGVPLGVSRGPEFARQFIARIEAQTRLPAS
                     TTINSAVEALRWLGAKRVIVLTPFPPATHERVISFLAAEGFSVQAEARMEARLKDLYL
                     IGEREVHEFMTGAVRRGPRADAVFAPCPQWHAFELTRVLERDTGLPFVTGDGCDFWYA
                     FRKLGITGVKPGYGRLLDSLGGPAMGERAGAG"
     gene            4326..5072
                     /locus_tag="AC16CX_06855"
     CDS             4326..5072
                     /locus_tag="AC16CX_06855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012269.1"
                     /translation="MPEVRFEVRGRVALVTGAASGIGRATALGLAGIGIDVGIVDRDA
                     EGIAGARAAIESRGNRVLGVVADVAQPADIQRAVREVSERLGPIDILVNCAGIFPRSL
                     VTGMARAEWDRVLATNLDGVFLASQAVLPAMIARKHGRIVSITSRLAVTGSPRGAHYA
                     ASKGAIDAFTRSLAREVAGDGININAIAPGLTDTPMMRGANSPEYIASVTQAMPHGRL
                     GQPEDVVGLVLFLVSEGARDITGQVIPMRG"
     gene            complement(5106..6068)
                     /locus_tag="AC16CX_06860"
     CDS             complement(5106..6068)
                     /locus_tag="AC16CX_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012270.1"
                     /translation="MKTVLLVVVAAIALVAAPAGQAREYPTKPIRLVVPFPPGGGVDI
                     LARVVGARVAESIGQAIVVDNRPGAGGTIGVETVVRANPDGYTLVMVNSNYSGTAAIQ
                     KLSYDPVNDIQPIILLGETGLVVVVHPSMPVKNIKEFIAYAQANPGRLNYASVGPGSL
                     THLALELFKLESKVGLTHVPYKGGAPALSAVIGGEVQVTAISMVPTVPHVKAGRLRAL
                     GVTTGKRLAALPEVPAVAETVPGYEANHWYGIWGPKGLPGKIVVRWNKEVAKVLQTAE
                     MKSRLADEGVEPAGGPPEQFLKTIKRDVEKWRRVVKEMKITAAG"
     gene            6079..6297
                     /locus_tag="AC16CX_06865"
     CDS             6079..6297
                     /locus_tag="AC16CX_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012271.1"
                     /translation="MKFVVVEVVLLELPGGDDVRHDTIERRRRSADSPTRRGASMRAA
                     EVVIVSGLGLDGCAIMTSGEGNKRRGNA"
     gene            6435..6875
                     /locus_tag="AC16CX_06870"
     CDS             6435..6875
                     /locus_tag="AC16CX_06870"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR3012272.1"
                     /translation="MYPQIHVNYLAVAAAVVVSFLFGWLWYGPLFGKKWAGLMKMPAD
                     FKPDPGLMMRSMGLTLLGTFLTAYVLLHSTEVWRASVWGAGTDAPSWRYGLFGGFFTW
                     LGFYVPLLLGSVAWEGRSWSLFGLNAAYHLVNLQLIAMILAHWR"
     gene            6986..7504
                     /locus_tag="AC16CX_06875"
     CDS             6986..7504
                     /locus_tag="AC16CX_06875"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3012273.1"
                     /translation="MKSKKWQVLTAMLALSPCAMGAQTLVTERTLSVNAAMEMATAAL
                     EACRKHETQVTITVLDHAGRTKVVLRDDGSNPHSVEHSLRKAYTALTYRMPSGEYGKR
                     AAGNFPASAGPLHLPNITTSAGGLPIRAGNTVVGAIGISGTPGAKGGGAADEACAQAG
                     IDRVSKGLGGGG"
     gene            7730..9199
                     /locus_tag="AC16CX_06880"
     CDS             7730..9199
                     /locus_tag="AC16CX_06880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3012274.1"
                     /translation="MQGSIGWIFRLSYLGQVGLLAAIYFVAAKASLAFAIPPGYATAV
                     WPPSGIALAAVLMLGERVWPGVWIGAALTNFTVASSLFASVAIGSGNALEALAGAALV
                     RRYIADAPPYLDRARNVVKFVAFAGLCSTVAATIGVGSLALEGAVSWPAFVPNWGTWW
                     HGDTAGIIIVTPLVLAWKLRHDIVWSPAKIWEAAGFASSLLLAAILIFGNGDRYFSSL
                     PLTFAILPFIIWAAFRFSRREMTMAIAAVCTIAVWYTIEGRGPFALDTLNLSLLSLLA
                     FISTVVTTGLILSAVVGERSRALERLAQALQGLREQASTDPLTDLTNRRYMWEFLQRE
                     WIRARRRKSSLAVIMIDLDHFKSVNDTFGHDAGDRVLTNVANLLKTHIRGSDIACRFG
                     GEEFALVLPDATLEGVQRRAEDIREAIGRLETEYRGRPLGRITASLGIALFPDHAGDP
                     ESLLRASDQALYGAKSTGRDRVVVSQAKPVLPTPSKQVN"
     gene            9387..>10126
                     /locus_tag="AC16CX_06885"
     CDS             9387..>10126
                     /locus_tag="AC16CX_06885"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3012275.1"
                     /translation="MADDVTQALARFAATLTYDRVPERAREYCKDVLLDTLACAVAGH
                     QGEETRQVSALAAGLAQSHESSIIGGDRSSLAGATLLNGYLITAVTMCDVHRPTLTHV
                     TPEVVPPALAIAERDSVSGRDLLVALAAGFETLTRVGIGWDYPAARAKGWHGPGVIGP
                     FGSAAAVGRLIGLDADTMAAAFGLAGSQAAGTFAAWGTPTVKFHQCRGALSGLMAALL
                     AQQEFVATREFLTARDGGLYNTYSNGGRP"
BASE COUNT         1713 a         3288 c         3333 g         1792 t
ORIGIN      
        1 tctccggcga gcagcgcgcg acccaggcgg aaatcgctgg cgacgcgctg gtgcactttg
       61 tagatgctct tgtactggat ggcgtagcgt cccgcctttg gctggaagcc ctgcatcagc
      121 cgctcgacgt tgtcctccga gagcgccgcc gcctcgtcct cttcgggcct gacgggaaac
      181 agcacgcgcc acagccccgg cggcccgtcg ccgggcatct tgaacatggc gttccagtcc
      241 acgggatcgg cgacgtaggt gttgagccgg aagccatgcg gctcgtaatc gtaattggtg
      301 ctcgccacca ggaagcgttc gggccaggtg tagccctcga atccgacgcc catgatcctg
      361 cgcaccgtgc tgcggccgcc gtccgctccg accagccacg cggcctcgac gcgctccatg
      421 ccttccggcg tcttcacggt caccacgacc ctgtcggcgg tctgcgcgaa accggtcacc
      481 tcgtgcgaaa acctgacctc ggcgttcggg cattccccga gcttcgagta gatgatgggc
      541 gtgagccggt gctgctccag gtgcagccgg taggggtacg gcgtcaggtc cttgatcagc
      601 gagacgtccc aatcgacgat gacaccctcc ttccggtcgc ggatctgcca gcgcggcacc
      661 ttgatcgcag tcttgagcat ctcgtcggta atcccgtacg gcgccatcat ttcgagcgac
      721 ggcggatggt aggtgcctgc gcgcaggtcc acggtgaggc cgggctcggc ctcgaacacc
      781 agcacggaga cctcctgtct tgcgagcgcg agcgcggtca gcaacccgac cggcccggcg
      841 ccggcgacga ttacctgggg gggcttcgtc atgatgtcgg gccctccccc gtcagcgtgt
      901 ccgcatcgag aacgccagga gggtcgcggt gacggccagc agcgcgagcg cgctcagcgc
      961 gagcgaccag tgaatgccga tcaggctgcc cagtatcccc accgtgatcc cgctgaacgc
     1021 gcgcaggcca ttgttggaca tctggaacag cccgatcagg cggccgcgca gatgggccgg
     1081 ggcttccaac tggaccagcg cctgcgccat ggagctgtat gcgaggctca ggaatccggc
     1141 cacgaacatg agcgcgaccg cgagcgggta gctcgcggtg gccgcgaagc cggcgatgct
     1201 gaagcaccac aggatggtga ggacaatcgc ggtgtgcggc gtggaacgga gcagccccct
     1261 ggactcgagg atcatgccgc cgatgaaagc acccgccgcg ttggaaccga gcaggacggc
     1321 ataggaaaaa tcgggcttct cggtgccgag gtcctgggcg tactccggca tctgcgccag
     1381 gaacgcgtta ccgacgaata gcgacgacaa cccggccagc aaaatcatgg acaggatcgt
     1441 gtggttgcct gaaacggcct gaagggtctt catcgcgtcg acgaatccgc ccgggcgtgc
     1501 cagcacgctt tcacgccgcc ggtccgcccc gtatggagct ttccacagcc accaggcgag
     1561 cggcagatag atcagcacgt ttaccagcag ccccgccgcc gggcccagca gcagcatgag
     1621 ccctccgccg acccccggcc ccaggaacac gcccagctgg cggcccgttg cgttcaaccg
     1681 caccgcgctc accaggtttt cccgcccgac tatgtcgtgg atcagcacct ggctcgccgg
     1741 cgcccacagc acccccgcca tcccatgcac gacgagcagg accgccgcgt gccacacctc
     1801 gatcgtgtcc gtgtagaaca aaacggccca cgctaaggac accaccatga acatgaacat
     1861 cgcgacttgg atgatgcgcc ggctgtcgaa gcggtcggcc atcgctccga agtagaccga
     1921 aaacagcagg aacggcagcc agtggctcaa caccgcgaat ccggcgaggg caggcgaatg
     1981 gaacttctcg aacatgaccc agtagctgat gacgtgctcg atgttgtcgg ccatcatcgc
     2041 cgccgccgtg gtgacgaaat agatccggaa gccgccgtgg cggagcgccg cgaacgaagg
     2101 cggcgccgcg cgctccgccg cgacgttaac ggctgggtca gtcatgagat cagggcgtgc
     2161 ttcgggaact tgcctgaatc ccgctagtca tccgtcatcc ctcattttcc ggccggcacg
     2221 acggcaataa cgtccatctc caccagcatg cccgggcgcg cgagctggct gatggcgagg
     2281 aaggtgtgca ccggcagcgg gccgcccttg aacgcttccg cccggcatct gttcatcgcg
     2341 tcgcggtcgc gaatgtccac gacgtaggtc acgagcttga tcacgtccgc catcgtcgcc
     2401 ccgtgcgccg cgaggagctt cctgatcttg tcgtaggcgt agcggcactg cgccgtgaag
     2461 tccccgatgt ggaggatcgc tccatccttc tcggcctcgg ctccgacgcc tgcgaggaag
     2521 acccacttcc cgggacccgt cacggtgacg gcctcggaga agcgtccctt ggtccattcg
     2581 ttgtggtgga agtgctgctt ctcgaacgcc tgcgcggcgg cgggcgcaca aaacaacgcg
     2641 atcgaaagca ggatcatcgt gcggacgaca gatttcaaca tggcacagct ccctttgggg
     2701 ttgaacggat tgaaaaggtg gatcgggcgc tacccgatcc gaccccgctt attctgtcac
     2761 gctttgcagg cgaatctagc cagtcaacga tccacgctcg ccgtctccct gaggaacacc
     2821 gggaggtcgg tcgtcggcgg gcgcgccggc acctgataga acaggtcggc cacgaagccg
     2881 cggtgatagc tcgtatggtt gacgacgtgg agcaggatct cgccgcgcgt catcacgccc
     2941 cggttgccgc cgatgagcgt gaagctcacc ttctcatcca gcgcggcgtc cgaaagccgg
     3001 tcgctccagg cgatgtacca ccggtcgatc tcctgctgcg cgcgccacag gtcggcgagc
     3061 ggcgggtgat ccggcgtgtt gcgcgcctca tagccgtgct cgcgcccttc aaggtgcgcc
     3121 tgccagatgc ggtctatgac gtagttgtgg ttgagcgtgt ggaccatgtt cttgaacagg
     3181 ctgcggcgct ccttcgtcgc ctcgccctcc ggcagcgcgg cgacggcgtc gaagatgagc
     3241 tgattcgccc acgcgttata gcgcgcgagc atgcgggcgg tgcgggtagt aatcatggct
     3301 gggaggattg aaggaggttg gcagttggca gttggcagtt ggcagacggc aattagcggt
     3361 tgccgcaatt tggcgggtga ccgctaccaa ctgtgtgcgc cacctgtcac gataacatgg
     3421 ccgaggtaaa ctggttcctg agaagcaact ggcgaccgct catggcgaag ccggtatcct
     3481 ttcctgtaat cttgctggcg ccggtcttgc atcaggagcg tgtcggggca ctgtccgaca
     3541 acctccccgg cgagatgcgg ctggaggtgc ggcgtgtttg cgtggcacaa gcggatcggg
     3601 cacatctgtc ccggggcaat ctcgacctcg gcttacgatt tttacctcgt ggcgccggag
     3661 ggcgtgggat tgttctgcgt gaccaatccg atcgaggact ggcgggacga cgagtacgag
     3721 caaagcctgg cgcgggttga cgaagcggca aactacctcg cgtcggtgca cgcggatttc
     3781 atcgctcatg tcggcgtgcc cctgggggtg agccggggcc ccgagttcgc gcgacaattc
     3841 atcgcccgca tcgaggcgca gacccggctg ccggcctcca cgacgatcaa ctccgccgtg
     3901 gaagcgttgc gctggctcgg ggcaaagcga gtcatcgtcc tcacgccgtt cccgcccgca
     3961 acgcacgagc gggtgatctc gttcctcgcc gctgaaggct tctcggtgca ggccgaggcg
     4021 aggatggagg cccggttgaa ggatctgtat ctcatcgggg agcgcgaagt gcacgagttc
     4081 atgaccggcg cggtgcgccg cggcccgcgg gccgacgcgg tgttcgcgcc ctgtccgcag
     4141 tggcacgcgt tcgagctcac ccgcgttctg gagcgggaca cggggttgcc gttcgtaacg
     4201 ggagacggct gcgatttctg gtatgcgttc aggaagctcg gcatcacggg agtgaaaccc
     4261 ggctacggca ggctgctcga cagcctgggc ggccccgcaa tgggcgaacg agcgggagcc
     4321 ggctgatgcc cgaagtgcgc ttcgaggttc gcggacgcgt tgcgctggtg accggcgcgg
     4381 cgagcggcat cggccgggca accgcccttg gcctggcagg catcggcatt gacgtgggca
     4441 tcgtggaccg ggacgcggag ggtatcgcgg gcgcgcgcgc ggcgatcgaa agccgcggaa
     4501 atcgggtgct cggggttgtg gcggacgtcg ctcagcctgc cgacatccag cgggcggtgc
     4561 gcgaagtgag cgaacggctg gggccgatcg atattctcgt caactgcgcc gggatattcc
     4621 cccgctcgct ggttaccggg atggccaggg ccgaatggga ccgcgtgctc gcgacgaacc
     4681 tcgatggcgt cttcctcgct tcgcaggcgg tgctgccggc gatgatcgcc cgcaagcacg
     4741 ggcgcatcgt tagcatcacc tcccgcctgg cggtgaccgg ctcgccccgc ggcgcccact
     4801 acgcggcgtc caagggcgcg attgacgcgt ttacccggtc gctcgcaaga gaagtcgccg
     4861 gtgacggcat caacatcaac gcgatcgccc cggggctcac cgatacgccg atgatgcggg
     4921 gcgcgaacag tcccgagtac atcgcttcgg tgacgcaagc gatgccgcac ggccggctcg
     4981 gccagccgga agacgtggtc ggattggtgc tgttcctggt cagcgagggc gcgagagaca
     5041 tcaccgggca ggtgattccc atgcggggct gagcgggcgg gcgcggcacg ccgtccgcgc
     5101 gtgaatcatc ctgccgccgt gatcttcatc tccttcacca cgcgccgcca tttttccacg
     5161 tcgcgcttga tcgtcttgag aaactgctcg ggcgggccgc cggcgggctc gaccccttcg
     5221 tccgccagcc ggcttttcat ctccgccgtc tgcaatacct tggcgacttc cttgttccac
     5281 cgcacgacga tcttccccgg caggcccttc ggtccccata tcccgtacca gtgattggcc
     5341 tcgtaaccgg gcacggtctc ggcgaccgcg ggcacctccg gcagcgccgc gagccgcttc
     5401 ccggtggtga cccccagcgc acgcaaccgc ccggccttca cgtgcggaac ggtcggaacc
     5461 atgctgatgg ccgtcacctg cacctcgccg ccgatcaccg cactcaacgc cggggccccg
     5521 cctttgtacg gaacgtgcgt gaggcccacc ttggattcca gcttgaacag ctccagcgcg
     5581 aggtgcgtca ggctgccggg gccgaccgag gcgtagttga gcctgccggg attcgcctga
     5641 gcgtaggcaa tgaactcctt gatgttcttg accggcatcg agggatgcac cacgaccacg
     5701 agccccgttt ccccgagcag gatgatcggc tggatatcgt tgaccggatc gtacgacagc
     5761 ttctggatcg cggcagtgcc gctgtagttg ctgttgacca tgaccagcgt gtagccgtcg
     5821 ggattggcgc gcaccaccgt ttcgacgccg atcgtgccgc cggcgccggg ccggttgtcc
     5881 accacgatcg cctgcccgat ggattcggcg acccgcgccc cgaccacgcg cgcaagtatg
     5941 tccacgccgc cgccgggggg aaaaggcacg accagccgta tcggcttggt cggatattcg
     6001 cgggcctggc cagccggcgc ggcaaccagc gcgatagcgg cgacaaccac gagcaataca
     6061 gttttcatcc gtccctccat gaaattcgtt gtggtggaag tcgtgcttct cgagctgccg
     6121 ggcggcgatg acgtgcgcca tgacacgatc gaacggcgtc gaagatcagc cgattcgccc
     6181 acgcgccgtg gcgcgagcat gcgggcggcg gaggtcgtca tcgtgtcggg actcggattg
     6241 gatgggtgcg caattatgac atccggagag gggaacaaga gaagaggaaa tgcgtgatag
     6301 gtgataagtg acaggtgctc ggggccggcg gagccggcca ttcttatcac gtgtcactca
     6361 tcacgcttta tcatgtcacg cccgattatg tcacgctgta ccatgtcacg tttcaccgtc
     6421 attaaggagg ggccatgtat ccgcaaatac acgtcaacta cctggcggtc gccgccgccg
     6481 tcgtcgtgag cttcctgttc ggatggctgt ggtacggacc gctgttcgga aagaaatggg
     6541 cgggcctcat gaaaatgccc gcggatttca agcccgatcc cgggctcatg atgcgctcca
     6601 tgggcctgac gctgctcggg acgttcctga ctgcctatgt gctgctgcat tcgacagaag
     6661 tctggcgcgc gtcggtctgg ggcgcgggaa cggatgcccc gtcctggcgc tatgggttgt
     6721 tcggcggttt cttcacctgg ctcggcttct acgtcccgct gctgctggga tcggtcgcgt
     6781 gggagggcag gagctggagc ctgttcggat tgaacgccgc gtatcatctc gtgaacctgc
     6841 agctgatcgc gatgattctc gctcattggc gttgagtaca ggattacgat cacgtcgtcc
     6901 cgccggacga cccggccgca tacggccttc ggcgcgttca tcaggggtgc aggttgaatc
     6961 caattcgcaa actacaggag gacacatgaa gagcaagaaa tggcaggttt tgacagcgat
     7021 gctggcgctt tctccgtgcg cgatgggcgc gcagactctc gttaccgagc gcaccctttc
     7081 cgtcaacgcg gcaatggaaa tggcgacagc agcgctggag gcgtgtcgca agcatgaaac
     7141 ccaggtgacc atcacggtgc tggaccacgc cggccgcacc aaggtcgtcc tgcgcgacga
     7201 cggttcgaat cctcacagcg tggagcacag cctgcgtaag gcgtacaccg cgctcaccta
     7261 ccgcatgccc tccggcgaat acggcaagcg cgcggccggt aatttccccg ccagcgccgg
     7321 tcccctccac ctccccaaca tcacgacgtc agcgggcggt ctgccgatcc gcgcgggcaa
     7381 taccgtggtc ggggcgatcg gcatttcggg aacgcccggc gccaagggcg gcggcgcggc
     7441 ggatgaagcg tgcgcgcagg ccgggatcga ccgcgtttcg aaggggctcg gcggcggcgg
     7501 ttaagcggat cagatacgcc ggcacaaagc agcaggagtc cagaatacga aatgattccg
     7561 ggaggggttt cgtgactttg atcacgaccc catccaattc atggacattc gtgaggatgc
     7621 aacagacaag cgtggttgcg tatgttatcg gtatccgcgc tgaacgctct gccattccag
     7681 aattcttata aagaaaggac agggggaata cggatgttcc ggggaaagta tgcagggttc
     7741 gatcggctgg atcttccggc tgtcctatct cgggcaggtg ggcctgctcg ccgccatcta
     7801 tttcgtggcg gccaaggcct cccttgcgtt cgccatcccg cccggctacg ccaccgccgt
     7861 gtggccgccg tccgggatcg cgctcgcggc ggtcctcatg ctcggggagc gcgtctggcc
     7921 gggagtctgg atcggcgcgg cgctcaccaa tttcaccgtc gcttcctcgc tgttcgcctc
     7981 cgtcgcgatc ggcagcggta acgcgctcga agcgctcgcc ggcgcggcgc tggtgcggcg
     8041 ctacatcgcc gacgcccccc cctatctcga tcgcgcgcgc aacgtcgtca agttcgtcgc
     8101 tttcgccggg ctttgctcca ccgtagccgc cacgataggg gtggggtcgc tcgcgctgga
     8161 gggcgcggtg tcgtggccgg cgttcgttcc gaactggggt acctggtggc atggggacac
     8221 cgccggcatc atcatcgtca cgccgctggt cctcgcttgg aagctgcgcc acgatatcgt
     8281 ctggtcgccg gcgaaaatat gggaggcggc cggcttcgca tcgtcgcttc tgctcgcagc
     8341 aatcctgatc ttcggtaacg gcgaccggta tttctcctcg ctgccgctca cgttcgcgat
     8401 cctgccgttc atcatctggg cggcgttccg cttcagccgg cgcgaaatga cgatggcgat
     8461 cgcggccgtg tgcaccatcg ccgtgtggta cacgatcgag ggacggggcc cgtttgcgct
     8521 ggatacgctc aacctgtcgc tgttgtcctt gctggccttc atcagcacag tggtcacaac
     8581 cggcctgata ttgagcgcgg tggtgggaga gcgaagccgt gcgctggaaa gactggcgca
     8641 ggcgctgcaa gggctgcggg aacaggccag caccgacccg ctgaccgacc tcaccaaccg
     8701 gcgctacatg tgggaattcc tgcagcgcga atggatcagg gcgaggcgca ggaaaagttc
     8761 cctcgccgta atcatgatcg acctcgacca tttcaagtcg gtcaacgaca ctttcgggca
     8821 cgacgccggg gatcgcgtgc tgacgaatgt cgcgaatttg ctcaagaccc acatccgggg
     8881 cagcgacatc gcgtgccgct tcgggggcga ggagttcgcg ctggtcctgc ccgacgccac
     8941 gctcgagggc gtgcagcgca gggccgagga catccgggag gccatcgggc gcctcgagac
     9001 ggaatatcgc ggcaggccgc tcggcaggat caccgcctcg ctcggcatcg cgctgttccc
     9061 ggaccatgca ggagatcccg aatcgctcct gcgcgcgtcg gatcaggccc tgtacggggc
     9121 caagagcacc ggccgcgacc gcgtcgtcgt cagccaggcg aaacccgttc tgccaacgcc
     9181 gtcgaagcaa gtcaactgaa accagaagcg tgatatgtga cgagtgataa gtgataagtt
     9241 acccgcgctt gcgcgggagt cagctcctat cacctatcac ctatcaccta tcacgctttt
     9301 ctggttttct tttagccctg cgggccttat gattggctcc ctaccctgtc tccattccaa
     9361 gaacgagacc gtccaaggag atttcaatgg cagatgatgt gacgcaggcg ctggcccgct
     9421 ttgccgctac gctgacctac gaccgggtcc cggagcgcgc gcgggagtac tgcaaggacg
     9481 tgctgctcga tacgcttgcg tgcgccgtgg ccggtcacca gggcgaagaa acccgccagg
     9541 tctctgcgct ggcggcgggg ttggcccaat cacacgagag cagcatcatc ggtggcgacc
     9601 gctcgtcgct ggcgggcgca acgctgctga acggttacct catcaccgcc gtcacgatgt
     9661 gcgacgttca ccgtcccacg ctcacgcacg tcactccgga agtggttccg cccgccctcg
     9721 ccatcgccga gcgcgattcg gtttcgggcc gcgatctgct ggtggcgctg gccgccggat
     9781 tcgagacgct gacgcgggtc ggcatcgggt gggactatcc cgcggcccgc gcgaagggct
     9841 ggcacggacc cggcgtcatc ggcccgttcg gttccgccgc ggcggtcggc cggctgatcg
     9901 gtctcgatgc cgataccatg gccgccgcct tcggcctggc gggcagccag gcggcgggca
     9961 cgttcgcggc gtggggcacg ccgacggtca agttccacca gtgccggggc gcactctcgg
    10021 gcctgatggc ggcgctgctg gcgcagcagg aattcgtggc gacgcgcgag tttctcaccg
    10081 cgcgcgacgg cggcctctac aacacctact cgaacggcgg acgacc
//
LOCUS       DBNGWZ010000230         1968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2794407, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000230 DBNGWZ010000000
VERSION     DBNGWZ010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2794407"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16CX_06890"
     CDS             <1..759
                     /locus_tag="AC16CX_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012276.1"
                     /translation="GAGGSIGTGQVAKAAPDGYTLLLGFVGQLAMYPHVEKVEYDPLR
                     DFVGVSLLASSYHVLAVHPSLPVRSVKQLIALAKARPGELNYASGNPWTPTHLVPELF
                     KSVTGTDIVAIQYKGSGPAAVAVISGDAQVIFGSVTAVMPHVRSKRLAALAVTSPARS
                     PIAPEVPTLVELGVRGVEAPSWYAIAAPAATPKDIVGRLHGELVKITALPEYRGLLQK
                     QALEPTSMTPEQFATFLQAEYDKWGKIIKSLKIQ"
     gene            complement(849..1931)
                     /locus_tag="AC16CX_06895"
     CDS             complement(849..1931)
                     /locus_tag="AC16CX_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3012277.1"
                     /translation="MKLKELGIAVVGAGRIGTLRARLAAKHPCVKFLAISDLDPERAR
                     ALAGQTGADFHSGNNFEVIERPEVNAVFVSTPEGEHAAPVRRALELGKPVLVEKPIAL
                     TLKDADGILETLKSTGGKLRIGYSRRFKECFLRAKEQMIHGRLGKVVGGLARVYNSRA
                     QTFAILKRDPHATPVLDVLTYYVDLMCWFLEGNRPVEVVARGQHGIFRDAGYTAHDAT
                     WAIVTFADGAVVNFGISYALPAKFPTQGQSDRVELLGTEGTMLIDDDHKDHLLYTEKG
                     IPHPYVPGHNLELVFLGSNTAGDWAVGDFWGPLGNETRSWLDHLVTGNPTAHTTPEQA
                     RVNLETTIAIERAVATGKAVRLPLET"
BASE COUNT          317 a          640 c          654 g          357 t
ORIGIN      
        1 ggcgccggcg gcagcatcgg caccggccag gtcgcgaaag ccgcgcctga cggctacacg
       61 ctgttgctcg ggtttgtcgg acagctcgcc atgtatccgc acgtggagaa agtcgaatac
      121 gacccgctcc gtgacttcgt cggggtaagc ctcctcgctt cctcgtacca cgtgctcgcg
      181 gtgcacccgt cgctgccggt acgttcggtg aagcaactga tcgcactggc caaggcgcgc
      241 ccgggcgagc tcaactatgc ttccggcaac ccctggacac ccacgcacct ggtgcccgag
      301 ctgttcaagt cggtgaccgg gaccgacatc gtcgctatcc agtacaaggg gtccggccct
      361 gcggctgtgg ctgtcatttc aggggatgcg caggtgatct tcgggagcgt gacggcggtg
      421 atgccgcacg tgcgctcgaa gcgtctggcg gcgctggcgg tgacgagccc cgcgcgctcg
      481 ccgatcgccc ccgaagtacc gacgctcgtg gaactggggg tgcgcggagt ggaggcgccc
      541 tcctggtacg cgatcgcggc gcccgccgcc acgccgaagg atatcgtcgg gagactgcac
      601 ggcgagctgg tcaaaatcac cgcccttccc gagtatcgcg ggttgctcca gaaacaggcg
      661 cttgagccga cctcgatgac gccggagcag tttgcgacgt tccttcaagc cgagtacgac
      721 aagtggggca agatcatcaa gtcgctcaag atccagtaag ccgggctcgc cttcagggtg
      781 acgcccggca agtgggcagt ttgcagttgc tgcttgctga cggctgacag ctgccaactg
      841 ccaacttttt aggtttcgag cggtagccgt accgctttgc cggttgcgac ggcgcgctcg
      901 atggcgatgg tggtctcaag attgacgcgc gcctgctcag gcgtggtgtg cgccgtgggg
      961 ttgccggtga cgaggtgatc gagccaggat ctcgtctcgt tgccgagggg gccccagaaa
     1021 tcgcccaccg cccaatcgcc cgcggtattg ctgccgagaa acaccagttc caggttgtgc
     1081 cctggcacgt agggatgggg gatgcctttc tcggtgtaaa gcaggtggtc cttgtggtcg
     1141 tcgtcgatca gcatggtgcc ttcggtgccg agcagctcga cgcggtcgga ttgcccctgg
     1201 gtcggaaact tggccggcag ggcgtaactg atgccgaagt tcaccaccgc gccgtcggca
     1261 aaggtgacga tcgcccaggt ggcgtcgtgt gcggtatagc cggcgtccct gaagatgccg
     1321 tgctgcccgc gcgccaccac ctccaccggc cggttgccct cgagaaacca gcacatcagg
     1381 tccacgtaat acgtgagcac gtccagcaca ggcgtcgcat gaggatcgcg cttcaggatc
     1441 gcgaaggtct gcgcgcgcga gttgtagacg cgcgccagcc cgccgaccac tttcccgagc
     1501 cggccgtgga tcatctgctc cttggcgcgc agaaagcatt ccttgaagcg gcggctgtag
     1561 ccgatacgaa gcttcccgcc ggtgctcttc agcgtctcga ggatgccgtc ggcatccttc
     1621 agcgtgagcg cgatcggctt ctcgaccagc accggcttgc ccagctccag cgcccggcgc
     1681 accggcgccg catgctcgcc ctcgggcgtg gagacgaaca cggcgttaac ttccggtcgt
     1741 tcgatcacct cgaagttgtt ccccgagtgg aaatccgcgc ccgtctggcc ggcgagcgcg
     1801 cgcgctcgct cggggtcgag gtccgagatg gcgagaaact tgacgcacgg atgtttcgca
     1861 gcgagccgcg cccgcagcgt gccgatgcgg ccggcgccga cgactgcgat gcccagttcc
     1921 ttgagtttca tcgagcgtcc tctccgatgt gcacgccggg cgtccagc
//
LOCUS       DBNGWZ010000231         3647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3046334, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000231 DBNGWZ010000000
VERSION     DBNGWZ010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3046334"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="AC16CX_06900"
     CDS             complement(<1..737)
                     /locus_tag="AC16CX_06900"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3012278.1"
                     /translation="MKTRRDLMLEEMGLTPVWRLRNRSDAAPADEAAVTESAASNNGP
                     VVSRQSSVVTGGDDRRAAIMRMEWPELKARVAGCTDCPLHAKRNKAVFGVGDENADWL
                     FVGEGPGADEDAQGEPFVGQAGKLLDNMLAAIKLRRGENVYIANCIKCRPPGNRNPEP
                     GELIACEPYLHRQIELIRPKLIIALGKVAAANLLATDASIASLRGRIHRYRGIPLIVT
                     YHPAYLLRNLPDKAKAWVDLCFAVRTMR"
     gene            complement(755..1219)
                     /gene="rimI"
                     /locus_tag="AC16CX_06905"
     CDS             complement(755..1219)
                     /gene="rimI"
                     /locus_tag="AC16CX_06905"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3012279.1"
                     /translation="MSAQLDMLPRYRRMTAADLEAVMMIEEAVYPYPWTRGNFADSLA
                     AGYHCWIVECGGEMAGYTVVTVAAGEAHLLNLSVAAPWQRQGIGRELLDFVLRLARDY
                     GVAKILLEVRPSNSAALSLYASADFAEIALRRGYYPAGDGREDAIVLQLELR"
     gene            complement(1216..1917)
                     /gene="tsaB"
                     /locus_tag="AC16CX_06910"
     CDS             complement(1216..1917)
                     /gene="tsaB"
                     /locus_tag="AC16CX_06910"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3012280.1"
                     /translation="MAGLEFTPGMKILALDTSSEYCSVALWRDGAVDARATLAGQRHS
                     ELLLPMVDELLKRHGLTVKELDGIAFGQGPGSFTGLRIGCGVTQGLAFGAGLRVVGVG
                     TLVAMAEAAQAGQAVCCLDARMGEIYHAAYAKSGMAWEPVHAPSLCAPAEAPALPAGS
                     WTGCGSGFAVHGAALKSRYAGRLSAIIPDVFPHAKDIARLAAREFEAGRAVPAEQAVP
                     VYIRDKVALRSDERR"
     gene            2049..3101
                     /locus_tag="AC16CX_06915"
     CDS             2049..3101
                     /locus_tag="AC16CX_06915"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR3012281.1"
                     /translation="MREYRIAAIPGDGIGVEVIAAGLKVLDELASRERGFRLRIERFP
                     WSSEYYLKHGHYIPEGGLDKLKQFDAIFFGAVGAPEVPDHISLWGLRLPICQGFDQYA
                     NVRPARVLPGIRSPLANGSAIDWVIVRENSEGEYSGSGGRAHRGLPEEVATETSVFTR
                     AGVTRIHRFAFGLARNRPRKHLTLVTKSNAQRHGMVMWDEIFYEVAKDYPDVVTQRVL
                     VDAMTTLMVLKPQTLDTIVATNLHADILSDLAAALSGSLGIAPTANLNPERKFPSMFE
                     PIHGSAFDIAGKGIANPIATFWSASMMLEHLGEKNAARRLMRAIETVTARRIHTPDLG
                     GEATTEIVTSAVCDAL"
     gene            3175..3489
                     /locus_tag="AC16CX_06920"
     CDS             3175..3489
                     /locus_tag="AC16CX_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012282.1"
                     /translation="MNQVFEHLKSFISIVLMLAGIGGVCWDLFKEGGWLGKILGKFWD
                     VQMNYPMIAIPVTIAVVVLGKMWRDHQVEKGHTSKLPDVFIYLVMAAGVYYLWRFSTD
                     LF"
BASE COUNT          628 a         1259 c         1150 g          610 t
ORIGIN      
        1 cgcatcgtcc tcacggcaaa gcacagatcc acccacgcct tggccttgtc cggcaggttg
       61 cgcagcaggt aggccggatg atacgtgacg atcagcggaa tgccgcggta gcggtggatc
      121 cgtccgcgca ggctggcgat gctggcatcg gtcgccagca aattggccgc ggcgaccttg
      181 cccaacgcga tgatcagttt cggccggatc aactcgatct ggcggtgcag gtaaggctcg
      241 caggcaatca gttcgcccgg ttccgggttg cggtttccag gaggacggca ttttatgcag
      301 ttcgcgatgt agacgttctc gccccgcctc aacttgatcg ccgcgagcat gttgtcgagc
      361 agcttgcccg cctgccccac gaagggctcg ccctgcgcgt cctcgtcggc acctggcccc
      421 tcgcccacga acagccagtc ggcgttctcg tcgccgacgc cgaacaccgc cttgttgcgc
      481 ttcgcgtgca gcgggcaatc cgtgcagccg gcaacccgcg ccttcagctc cggccactcc
      541 atgcgcatga tggccgcgcg ccggtcatct ccgccggtga cgactgacga ctgacggctg
      601 acgacagggc cattgttgga tgcggccgat tcagtcaccg ccgcttcatc ggcgggcgcc
      661 gcatcgctgc ggttgcgcag gcgccatacc ggcgtcagcc ccatctcctc cagcatcaga
      721 tcacggcggg ttttcactcg tcagtcgtca gtcgtcaccg cagttcgagc tgcagcacga
      781 tagcgtcctc ccgaccatct cccgccggat agtagccgcg gcgcagcgcg atctcggcga
      841 agtccgcgga cgcatacagg gaaagcgccg cgctgttcga agggcgcact tccagaagga
      901 ttttcgcgac accgtagtcg cgcgcgagcc tgagcacgaa gtcgagcagc tcgcgaccga
      961 tgccctgccg ctgccacggc gcggccacgc tcaggttgag gagatgcgcc tctccggcag
     1021 cgaccgtcac cacggtatac ccggccatct cgccgccgca ttcgacgatc cagcagtgat
     1081 aacccgccgc gagcgagtcg gcgaaattgc cgcgcgtcca gggatacgga tagaccgcct
     1141 cctcgatcat catgactgcc tcgaggtcgg cggcggtcat gcgccggtaa cgggggagca
     1201 tgtccaactg cgcgctcatc gccgttcgtc actcctcaac gccaccttgt cgcggatgta
     1261 caccggcacc gcctgctcgg cggggaccgc cctgcctgcc tcgaattccc gcgccgcgag
     1321 ccgtgcgatg tccttcgcgt gcgggaacac atccgggatg atagccgaga ggcgccccgc
     1381 gtagcggctt ttcagcgccg ccccgtgcac ggcgaatccg cttccgcagc cggtccacga
     1441 tccggccggc aatgccggcg cctccgcggg agcgcaaaga ctgggggcat gcaccggctc
     1501 ccacgccatg ccggatttcg cgtaggcggc gtggtagatc tcgcccatgc gggcgtcgag
     1561 gcagcacacc gcctgcccgg cctgggccgc ctcggccatc gcgacgagcg tgcccacgcc
     1621 caccacacgc aacccggcgc caaaagcgag cccctgcgtc acgccgcagc cgatgcgcag
     1681 cccggtgaac gaccccgggc cctgaccgaa ggcaattccg tccagctctt tcaccgtgag
     1741 gccgtgccgt ttgagcagtt cgtccaccat gggcagcagc agctcggaat ggcgctggcc
     1801 ggcgagcgtc gcgcgcgcat ccacggcccc gtcgcgccac agcgcgacgg agcagtactc
     1861 ggaagaggtg tcgagcgcga ggatcttcat gccaggcgtg aattctagcc cggccatcgc
     1921 gccagcgtat aatgcgcgac ccaaacttgg taagcggtga gcagtcagcg gtgagccgaa
     1981 aaagccgtca cttgctgtct cttagactcc gtttaccgct tactgcttac cgcttacgga
     2041 ttcttgaaat gcgcgaatac agaatagccg ccattcccgg cgacggcatc ggcgtcgagg
     2101 tcatcgccgc cgggctgaag gtgctggacg agctggcgag ccgcgagcgc ggcttccggt
     2161 tgaggatcga gcgctttcct tggagcagcg agtactacct caagcacggc cactacatcc
     2221 ccgagggtgg tctcgacaag ctcaagcagt tcgacgccat cttcttcggc gcggtcggcg
     2281 cgcccgaggt acctgaccac atctcgctgt gggggctgcg gctgccgata tgccagggct
     2341 tcgaccagta cgccaacgtg cggcccgcgc gcgtgctgcc ggggatccga agcccgctcg
     2401 cgaacgggtc cgcgatcgac tgggtcatcg tccgcgagaa ctccgagggc gagtactccg
     2461 gcagcggcgg gcgcgcgcac cgcggcctgc cggaagaggt cgcgaccgaa acctccgtgt
     2521 tcacccgcgc cggggtgacg cgcatccacc gcttcgcgtt cggacttgcg cgcaaccgcc
     2581 cgcgcaagca ccttacgctc gtcaccaagt cgaacgcgca gcgccacggc atggtgatgt
     2641 gggacgagat tttctacgag gtggcgaagg actatccgga cgtcgtgacg cagcgcgtgc
     2701 tggtggacgc gatgaccacg ctgatggtgc tgaagccgca gacgctggat accatcgtcg
     2761 ccaccaacct gcacgccgac atcctgtccg atctcgccgc ggcgctttcg ggcagcctcg
     2821 gcatcgcgcc gaccgcgaac ctgaatccgg agcggaaatt cccctcgatg ttcgagccga
     2881 tccacggctc ggctttcgac atcgccggca agggtatcgc caacccgatc gcgacgttct
     2941 ggagcgcgtc catgatgctc gagcatctgg gcgagaagaa tgccgcgcgg cggctgatgc
     3001 gcgccatcga gacggtgacc gccaggcgca tccacacacc cgacctgggc ggcgaggcga
     3061 cgactgaaat cgtcacttcc gcggtatgcg acgcactgtg accggcttcc accccgtcgc
     3121 gagcgggcgc tgatacaata gacgtgcatc gcgcaaccaa ccgggggtaa gcgcgtgaat
     3181 caggtcttcg agcacctcaa gtccttcatc tccatcgtac tgatgctggc gggaatcggc
     3241 ggcgtgtgct gggacctgtt caaggaagga gggtggctcg ggaagatcct cggcaagttc
     3301 tgggatgtgc aaatgaacta tcccatgatc gcgataccgg tcacgatcgc ggtggtcgtc
     3361 ctcggcaaga tgtggcgcga ccaccaggtc gaaaagggtc acacgagcaa gctgcccgat
     3421 gtgttcatct acctcgtgat ggcggccggg gtctattacc tctggcgctt ctccaccgac
     3481 cttttctaga agcagcgctc cgcgccggcc tcaggcggca cgcacgatcc gccgcgagac
     3541 caggtcgtcc acagcttccc tgctgtagcc cagtcccccc agcaattcgc gagtgtgctc
     3601 gccgagtttc ggcacgtcga gccgcgtcgc gaaacggcgt ccgtcca
//
LOCUS       DBNGWZ010000232        17282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2667695, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000232 DBNGWZ010000000
VERSION     DBNGWZ010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2667695"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="AC16CX_06925"
     CDS             complement(<1..1523)
                     /locus_tag="AC16CX_06925"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3012283.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQKLIDLDIEIPGKEQRKSNIYKG
                     VITRIEPSLEAAFVDYGAERHGFLPFKEVARAYFKPGVDVGRARIQDALGESQELIVQ
                     VDKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRVEGEDRTELREIIDQLPVP
                     QGMSVIGRTAAIGRAHEELNWDLNYLMQLWRAIEGAAQQQSGAFLIYQESSLVIRAIR
                     DYYHQDIGEVLIDTESVYEQARQFMSHVMPQNVNCIKLYRDDVPLFSRFQIEHQIESA
                     YSRSVNLPSGGAVVFDHTEALVSIDVNSARATRGSDIEETAFRTNLEAAEEIARQLRL
                     RDLGGLIVIDFIDMESGRNQRDVENRLRDSLRYDRARVQTGKISRFGLMELSRQRLQT
                     SLGETSHIACPRCHGTGHIRSIESSALHILRILQEEAMKENTAAVHAQVPVDVATYLL
                     NEKRSEFHAIESRMKVNVVLVPNIHLETPNYTLTRLRHDELNQMEPLPASYQLMEQPA
                     "
     gene            2016..2276
                     /locus_tag="AC16CX_06930"
     CDS             2016..2276
                     /locus_tag="AC16CX_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012284.1"
                     /translation="MRILNLTNPVRGLDSKKASYLLARLERFFFGFGVKGCGGVLSIR
                     RSTSSGLGARSGFDFDKYISNAANAACDLSFGSVGAFMVEGV"
     gene            complement(2392..2838)
                     /locus_tag="AC16CX_06935"
     CDS             complement(2392..2838)
                     /locus_tag="AC16CX_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012285.1"
                     /translation="MLLGEIAFYTSHIEYHLHQVLWHLLEVNPKRGMAITGPMQFNQK
                     VDLLLSLIEEMPEKDHALISAAKMIEKARPERNRKLHAFWIWEGKTRRKRAAILLSSN
                     RRLFKLVPVSYKDIEEARDITILASGLLNSWLWSKNLMGKVSMPFE"
     gene            complement(2900..3385)
                     /locus_tag="AC16CX_06940"
     CDS             complement(2900..3385)
                     /locus_tag="AC16CX_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012286.1"
                     /translation="MPDIALIQSAITGLKTAADIAKGILDLKTMAEVQGKIIELQAAI
                     LSAQSSALAAQSDQFAMVEEIRALKEEIARVKAWEEQKQRYALYEVRSNRFVYLLKES
                     RKGTEPPHWICAKCYEDGRKSVLQFQGTYFVDTHCLTCHLCKNTIPMHEGIPELKYFP
                     D"
     gene            complement(3491..4405)
                     /locus_tag="AC16CX_06945"
     CDS             complement(3491..4405)
                     /locus_tag="AC16CX_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR3012287.1"
                     /translation="MAKSILTELRFKDDEAARKHLEAIRWPNGPVCPHCGGIERNSLL
                     NGSSHRSGLYFCGDCRTQFTVTVGTVFERSKIPLHKWVLATHLLCASKKGMSSKQIER
                     MLGVTYKTAWFMTHRIREAMTGNPSSLLGSGGGTVEADETYWGNKKEYGKGPKGWHHK
                     MKVLSLVERNGEARSFHVPSVNSKTLKPILQAQIAKEASVMTDDAVVYRSAARGFASH
                     QSVNHSIGEYSRVGAHTNTVESYFSLLKRGLIGTFHHVGEQHLQRYVKEFDFRWNHRK
                     ITDAERAAFALKGISGKRLTYRRINGTA"
     gene            4465..5424
                     /locus_tag="AC16CX_06950"
     CDS             4465..5424
                     /locus_tag="AC16CX_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3012288.1"
                     /translation="MTKESATWHEVEVEAAGQRVDNYLTKRLKGVPKSHIYRILRSGE
                     VRVNSRRVGPEYRVQEGDRLRLPPVRTAKPAAVPKQPPSTRLKAKVLYEDDVLLVIDK
                     PAGVAVHGGSGISRGVVEQLRLERPEARFLELVHRLDRETSGVLMLAKRRSALTELHR
                     QLREGRVQKSYLALVKGAWRDGKRSIKLPLHKYVLASGERRVSVSRDGLPAHTIFRLQ
                     RSWSGYSLLEAELKTGRTHQIRVHLAHVGYPVAGDDKYGDFALNKQLARQGLKRMFLH
                     AFKIVIAHPQSGAVLTLEAPLPPDLQAFLAALNLEEARLAQKL"
     gene            5408..6064
                     /locus_tag="AC16CX_06955"
     CDS             5408..6064
                     /locus_tag="AC16CX_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3012289.1"
                     /translation="MPKNFDLLVFDWDGTLMDSAAAITASLQAACRDLELPVPNEERA
                     RYVIGLGLNDAMAHILPGLDPARYPEVVARYRVHFLERDSDTALFPGAAPVVVALHGA
                     GFLLAVATGKSRRGLERALAATELERYFHATRCADEGHAKPHPGMLLGLMEQLGVTAG
                     RTLMIGDTTHDMEMARAAGVPRLGAAYGAHSKEALLGYGPVACVDDFEGLRQWLMRNA
                     "
     gene            6154..7113
                     /locus_tag="AC16CX_06960"
     CDS             6154..7113
                     /locus_tag="AC16CX_06960"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HIR3012290.1"
                     /translation="MAELEPGGTGPSWERQAIEKVALAAIEEQRRSRRWGIFFKLLLF
                     IYLFALLFVGMGWIGKREVSPGKHTALVEVQGVISSNSPASAENITTGLQDAFKDKRT
                     QGVVLRINSPGGSPVQAGHINDEIRRLRAKYPDIPLYAVVEDVCASGGYYIAVAADRI
                     YVDKASIIGSIGVLMDGFGFTGTMEKLGVERRLLAAGENKGFLDPFSPILESQKQHAE
                     QMLNQIHQQFITVVRQGRGKRLKETPEMFSGLLWVGQKSVELGLADALGSVDYVAREV
                     IKAEDIVDFTPRENVAERLARRFGAGMADGLAHFGVFQGMTLR"
     gene            complement(7143..7868)
                     /locus_tag="AC16CX_06965"
     CDS             complement(7143..7868)
                     /locus_tag="AC16CX_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3012291.1"
                     /translation="MSEPRAGNGTLYLIPVALGREDALSNLPSATLDVTRSLRTFIVE
                     NAKSARRFLKAVHHPRPLQELSFHLFDEHANEDDVESLLALLLKGENCGLMSESGCPA
                     VADPGAALVRRAHEHGVRVVPLVGPSSILLALMASGMDGQHFTFHGYLPVDRAERARK
                     LKDIETRARGETQIFIETPYRSSAMLNALLEHCRGDTLLCIAADLTLPTESVTTRPVA
                     EWKKKPPDLDRRLVVFLVLRERG"
     gene            complement(7861..8445)
                     /locus_tag="AC16CX_06970"
     CDS             complement(7861..8445)
                     /locus_tag="AC16CX_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HIR3012292.1"
                     /translation="MDSPRIVLASSSPYRRALLMRLRLPFEVKAPAVDETALPGEAAR
                     DTALRLAQAKALAAAPSSSKALVIGCDQVAALDGACLSKPGSYENALAQLKAMRGKRV
                     IFHTALALLNTESGGMQTADVPTTVYFRNYSDREIERYLELEQPYDCAGSARIEGLGI
                     VLVERVVGDDPSALIGLPLMRLATMLRKEGVAIV"
     gene            8538..9029
                     /locus_tag="AC16CX_06975"
     CDS             8538..9029
                     /locus_tag="AC16CX_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3012293.1"
                     /translation="MTVIDSLEFARTGQTLSGSLPVPALTRLRDSLFDDLGEVRFEVK
                     GAHDARRRPILMLEISGVLHLQCQRCLGMLDYPLRITNTLLLAGAGAAAAGSLDEEDL
                     EWIEANVDLDVASLVEDEVLLSLPYAPRHAEGLCRHGRSGEAGDAAASAFAKLAALKR
                     NNN"
     gene            9049..9228
                     /gene="rpmF"
                     /locus_tag="AC16CX_06980"
     CDS             9049..9228
                     /gene="rpmF"
                     /locus_tag="AC16CX_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3012294.1"
                     /translation="MAVQQNRKSPSKRGMHRSHDALSSPPLALEPTTGEVHLRHHISP
                     NGYYRGKKVVKTRGE"
     gene            9307..10323
                     /gene="plsX"
                     /locus_tag="AC16CX_06985"
     CDS             9307..10323
                     /gene="plsX"
                     /locus_tag="AC16CX_06985"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3012295.1"
                     /translation="MIVKVAIDCMGGDHGAHVTVPAALGVLRDDPEISFLLVGRQDAI
                     ERELVRCGPAPEGRTSVRHASQVVEMDEAPVAAMRGKKDSSMRVAINLVEQGEAAACV
                     SAGNTGALMAVSRFVLGTLPGIDRPAIATMLPTLKGHTYILDLGANVNCSPEHLLQFG
                     IMCSELVSSIEGKPRPTVGLLNIGVEDIKGNELVKQAAELMRTSDLNFHGYVEGDDIY
                     KGTTDIVVCDGFVGNVALKTSEGLAQMLATYLREEFGRNPFTRLAGLVALPVISAFKH
                     RVDHRRYNGASLLGLRGIVVKSHGSADAFAFGFAIRRAIEEARTGVLAHISQRMAAIH
                     KSAA"
     gene            10320..11282
                     /locus_tag="AC16CX_06990"
     CDS             10320..11282
                     /locus_tag="AC16CX_06990"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3012296.1"
                     /translation="MSSYSRIIGTGGYLPAKVLANHDLERMVDTTDEWIYTRTGIRQR
                     HVAADDEKTSDLALHASRRALEAAGVAVADVDLIVVATTTPDMVFPSTACILQSKLGV
                     RNCPAFDVQAVCSGFIYGLATADQFLRSGQYRSAVVVGAEIYSHILDWKDRSTCVLFG
                     DGAGAVVLRRDNAPGILSSHLHADGSYANMLSVPGTVSGGRVSGRPLLQMDGGGVFKF
                     AVKALDDLVEEALAANGMQKADIDWLVPHQANIRIIQASAKKLGMSMEKVVVTVDRHA
                     NTSAASIPLALDEAVRDGRIRSGQHVLLEAVGGGFTWGAVLARW"
     gene            11313..12245
                     /gene="fabD"
                     /locus_tag="AC16CX_06995"
     CDS             11313..12245
                     /gene="fabD"
                     /locus_tag="AC16CX_06995"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3012297.1"
                     /translation="MKFAFVFPGQGSQSVGMMQPFSESKGARAVFTEASDVLKQDLWE
                     LVAEGPADELNSTVNTQPVMLTAGYAVYRAWRDAGGAEPALVAGHSLGEYTALVAAGA
                     LAFRDALPLVRFRAQAMQEAVPVGAGAMAAILGLEDDAVHAACSEAAQGQVVEPVNFN
                     APSQVVIAGHKAAVERGVAAAKVKGARRAVLLPVSAPFHSSLLRPAADKLAVYLGKVG
                     VSAPRIPVVNNVDVATESQPQRIKDALARQACNPVRWVEVVRRIAGSGIAHVAECGPG
                     KVLAGLTRRINGNLQGYAIADPQSLEQALQAFKS"
     gene            12233..12985
                     /gene="fabG"
                     /locus_tag="AC16CX_07000"
     CDS             12233..12985
                     /gene="fabG"
                     /locus_tag="AC16CX_07000"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR3012298.1"
                     /translation="MQIVSLDGKIALITGASRGIGQAIALELGRHGAVVVGTSTTQAG
                     ADGLQQRLSAAGIQGAGMALNVNDARQSEAVIAEIQKRFGDITILVNNAGVTRDNLLV
                     RMKDEEWDEIMATDLKSVYRLSKLVLRSMMKARYGRIINITSVVGVTGNAGQTNYAAA
                     KAGIIGFSKSLAREVGSRNITVNCIAPGFIETDMTRALNEQQRGALIESIPLGRLGRP
                     EDVAAAVAFLVSPGADYMTGCTLHVNGGMHMD"
     gene            13248..13487
                     /gene="acpP"
                     /locus_tag="AC16CX_07005"
     CDS             13248..13487
                     /gene="acpP"
                     /locus_tag="AC16CX_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3012299.1"
                     /translation="MENIEHRVKKIVAEQLGVNEADVKNESSFVNDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKIATVQQAIDYIKAHVKS"
     gene            13512..14750
                     /gene="fabF"
                     /locus_tag="AC16CX_07010"
     CDS             13512..14750
                     /gene="fabF"
                     /locus_tag="AC16CX_07010"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3012300.1"
                     /translation="MSRRRVVVTGLGIVSPVGTGAPEAWKNILAGQSGITRITRFDPS
                     AFSSQIAGEVKGFDPAQYLSAKEARRFDTFIHYGLAASIEAIKDAGLDFERESREQVG
                     ACIGSGIGGLPLIEEMHNAFLAGGPRKISPFFVPGTIINLIAGQLSIMYGLKGPNLAL
                     VTACTTANHCIGDAGRLIEYGDADVMIAGGSEACVSPLGVGGFCAARALSSRNDDPAA
                     ASRPWDRDRDGFVLGEGAGILVLEEYGHASKRGARIYCELAGYGMSADAHHITAPCED
                     GEGAVRCMANAVRNAGINRDEVDYINAHGTSTPLGDVAETVAVKDCFGGHAKKLAVSS
                     TKSMTGHLLGAAGGVEAVFTALAVRDQVAPPTINLVNPDPQCDLDYVPNAARKMKIGV
                     AISNSFGFGGTNGSLVFRRI"
     gene            14850..15863
                     /gene="mltG"
                     /locus_tag="AC16CX_07015"
     CDS             14850..15863
                     /gene="mltG"
                     /locus_tag="AC16CX_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3012301.1"
                     /translation="MKKGLERFLRVLLSASVAGIVIASGAFTYYATTEIDVGPSPIQF
                     NLKTGSSLRSAAQQMERAGVLRHPGIFTVMARLLGEAGNIKAGIYEISGPVTPYQLLL
                     KVTEGDVTQSTITLVEGWTFRQMRKLLDDHHAVKHDTRGLPDAEVLRRLAIDAASPEG
                     LFFPDTYHFNVGASDFLILKSAYRLMQSHLKSQWAGRAPDLPLASPYEALILASIVEK
                     ETGRVEDRPLIAAVFVNRLRKGMLLQADPTVIYGMGEAFDGNLRKHDLAMDTPYNTYT
                     RAGLPPTPIAMPGLTSLTVTLNPPRSDVLYFVSRGDGSSHFSRTLGEHERAVTKYQRL
                     GRR"
     gene            15863..16483
                     /gene="tmk"
                     /locus_tag="AC16CX_07020"
     CDS             15863..16483
                     /gene="tmk"
                     /locus_tag="AC16CX_07020"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3012302.1"
                     /translation="MRGKFITLEGLDGAGKSTHLRWIARFLRGRGIQVKLTREPGGTA
                     AGEKLRRLLLGRRQELHPETETLLMFAARREHLDKVIIPALNAGRWVLCDRFTDATYA
                     YQSGGSGVGWNKVAELEQWVQADLQPDLTLIFDVSPEVGRQRAGRQRRKPDRFEREQD
                     KYHQRVREAYLRRARENAERVHVVDANGSLPEVHKELERLISSFCK"
     gene            16483..>17282
                     /gene="holB"
                     /locus_tag="AC16CX_07025"
     CDS             16483..>17282
                     /gene="holB"
                     /locus_tag="AC16CX_07025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR3012303.1"
                     /translation="MSYSWHKPDGDALLRRKDRLPHALLLRGPQGIGKLAFAEALARA
                     LLCERPEPDGSACGHCTACGWMEQGAHPDLRRLEPEGLAEQDEAEQGGEKKASVQIQV
                     EQVRGIGGFINISSHRGGVKVVLIHPAETLNINAANALLKNLEEPPPRTYFLLVAHRW
                     HQLPPTIRSRCQHIALPPPPAETARSWLKQQGLRDPDLALAQAGGAPLLALKFDEEYW
                     RERELFIKAVADNGFDALRLAEQFRGQEPALIVSWLQKWSFDLVCHKLT"
BASE COUNT         3198 a         5218 c         5656 g         3210 t
ORIGIN      
        1 gccggctgtt ccatcagctg gtagctggct ggcaacggct ccatctggtt caactcgtcg
       61 tgccgcagcc gggtcagcgt gtagttgggc gtctccaggt ggatgttggg caccaggaca
      121 acgttcactt tcatgcgcga ttcgatggcg tggaactcgg agcgcttctc gttgagcaga
      181 taagtggcaa catccaccgg cacctgcgca tgcacggcgg cggtgttctc cttcatggcc
      241 tcttcctgca ggatgcgcag gatgtgcaag gcggaggatt cgatgctgcg gatgtgcccg
      301 gtaccgtggc agcgcgggca cgcgatgtgg ctggtttcgc ccagcgaagt ctggagccgc
      361 tggcgcgaca gctccatcag gccgaagcgc gagatcttgc ccgtctgcac ccgcgcccgg
      421 tcgtagcgca gcgagtcgcg cagccggttt tccacgtcgc gctggttgcg gccgctttcc
      481 atgtcgatga agtcgatcac gatgagtccg ccgaggtcgc gcagcctcag ctggcgcgcg
      541 atttcctccg cggcctcgag attggtgcga aatgccgttt cctcgatgtc ggacccgcgc
      601 gtcgcgcggg cggagttgac gtcgatggac accagcgctt cggtgtggtc gaacaccacc
      661 gcgccgcccg agggcaggtt gaccgagcgc gagtaggccg actcgatctg gtgctcgatc
      721 tggaagcgcg agaacagcgg cacgtcgtcg cggtagagct tgatgcagtt gacgttctgc
      781 ggcatgacgt ggctcatgaa ttgccgcgcc tgctcgtaga cgctctcggt gtcgatcagg
      841 acttcgccga tgtcctggtg atagtagtcc cggatcgcgc ggatcacgag gctcgattcc
      901 tggtagatca ggaacgcgcc gctttgctgc tgggcggctc cctcgatcgc gcgccacagc
      961 tgcatcaggt agttgaggtc ccagttgagt tcctcgtgcg cgcgtccgat cgccgcggta
     1021 cgtccgatga cgctcatgcc ctggggcacg ggcagctggt cgatgatttc acgcagctcg
     1081 gtgcggtcct caccctcgac ccggcgtgat acgccgccgc cgcgcggatt gttcggcatc
     1141 agcacaagat agcggccggc gaggctgatg aaggtggtga gcgctgcgcc cttgttgccg
     1201 cgctcgtcct tgtccacctg gacaatcagc tcctggcttt ccccgagcgc gtcctggatg
     1261 cgggcgcggc cgacgtcaac gccgggcttg aagtaggcgc gggcaacctc cttgaaaggc
     1321 aggaaaccgt gacgctcggc cccgtagtcc acgaaagcgg cctcgaggct gggctcgatg
     1381 cgggtgatga cacccttgta gatgttgctc ttgcgctgct ccttgccggg gatctcgatg
     1441 tcgaggtcga tgagcttctg accgtcgacg atcgcgacgc gcagttcttc tgcctgcgtc
     1501 gcgttaaaca gcatgcgttt cattgttatc ttctcccgcg ctcgaacacg ggatggggca
     1561 aaccacgggg cctgcgttca ggccgctcgt gctgactgga tcagtcgcgt ggcatgagca
     1621 tgatggatag gaatatccgg atgtctcgtg cggtttgccg cggacacccg cgccgggcgc
     1681 ggatgccgta aattcctacg gtgtcgtgag cgcgtatatc gtctagtatc gctacttctg
     1741 cgtggtgagc gacattaacc attccctgcg gcgaattcct gatcggtgcg gcttttcctt
     1801 ggagccattc agcctgaact tcggaatcgc attcagcacg cttactctgg ttcgtgtcct
     1861 ctgccgttta ctccggatgc tgggcacaag ccggctcgcg ggacgggatt ggcggagctg
     1921 ttcgcatcac gccgccctgc aatctgccgc gagcttggaa tagtatatac aaatgaaagg
     1981 gttaagtaaa gaatccgcca ttatataggt gacaaatgcg gattctgaac ttaaccaacc
     2041 ccgtcagggg tctagatagc aaaaaggcca gctatttgct ggcccgtttg gagcgcttct
     2101 tcttcggctt cggggtgaag ggttgcgggg gtgtattgag catccgccgc agcacttcgt
     2161 ccgggctggg agcccgttcg ggttttgatt ttgacaaata catctccaat gcagcaaacg
     2221 cggcctgtga tctttctttc gggtcggttg gcgctttcat ggtggaagga gtctaggccg
     2281 tgaccaatga aaaatcaatt gatattacgc ccgagatgat caaggctgcg atgcgtgaat
     2341 tagatggatg ggaaaatgaa catgatactc gtgaggacgc ggtgatcaga attattcaaa
     2401 cggcattgaa actttgccca tcaaattttt tgaccaaagc caagaattca aaagtccgct
     2461 cgcaagaatc gtgatgtcgc gtgcttcttc tatgtcttta tatgaaactg gaacgagttt
     2521 gaacaaacga cgattgctgc tcagaagtat ggcggctcgt ttcctgcgtg tttttccttc
     2581 ccatatccaa aatgcatgga gttttctatt tcgttctggc ctagctttct ctatcatttt
     2641 ggcagcagaa atcaaggcgt ggtccttttc tggcatttct tcaatgagcg agagtaacaa
     2701 gtcaaccttt tggttgaatt gcatcggccc agtgatcgcc atccctctct tgggatttac
     2761 ttcgagcaag tgccagagaa cctgatgcag atgatattca atatgcgatg tataaaaggc
     2821 tatctcgcca agcagcacca attccttttt tgtaactgga gcgcgtcgtc taagtcgaat
     2881 cacgtgaggt gataaaaaac taatcgggga aatatttgag ctccggaata ccctcatgca
     2941 ttggtattgt attcttacac aaatgacagg ttaggcaatg cgtatcaaca aaataagttc
     3001 cttggaattg gaggacagac tttcgcccgt cctcgtaaca ttttgcacat atccaatgcg
     3061 gtggttcggt tcccttgcgc gattctttca gcaagtaaac aaaacggtta gaccggactt
     3121 cgtaaagtgc atagcgttgc ttttgttctt cccatgcttt tacgcgagcg atttcttctt
     3181 taagtgcgcg tatctcctca accatcgcaa attggtcaga ctgggccgca agtgcgctgc
     3241 tttgggcgga caggatggca gcttgaagct caataatctt tccttggact tccgccatcg
     3301 tcttgagatc aagaatgccc ttggcgatgt ctgccgcagt cttgagtccg gtaatcgcac
     3361 tttgaataag ggcgatgtca ggcatgattc ctccccaaat cagaaatcaa gctcaagctg
     3421 aatccacttt cctatgttgc ccttagccac tatctgacgc agcaggtgag cgatgattgc
     3481 atatagcctc ttacgctgtc ccgttaatcc gccgataggt gaggcgcttg ccgctgatgc
     3541 ccttgagcgc aaaggcggca cgctcggcat cggtaatctt gcgatgattc cagcggaagt
     3601 cgaattcctt gacgtagcgt tggagatgtt gttccccgac gtgatggaac gtcccaatca
     3661 gtccacgctt gagcaagctg aaataactct cgaccgtgtt ggtgtgtgct ccgacgcggg
     3721 agtattcccc gatagagtgg ttgaccgatt ggtggctggc gaagccacgg gctgcgctgc
     3781 gatagacaac tgcgtcatcg gtcatcacgc tggcttcctt ggcgatctgc gcttgcagga
     3841 tgggcttcag cgtcttggaa ttgacgcttg gcacatggaa ggaacgcgct tccccgttgc
     3901 gctcgacaag ggaaagcacc ttcatcttgt gatgccagcc tttcggaccc ttgccgtatt
     3961 ccttcttgtt accccagtag gtttcatcgg cttccacggt gccgccgccg gagcccaaca
     4021 ggctggacgg gttgccggtc atggcttcgc ggatgcgatg ggtcatgaac caggcggtct
     4081 tataggtcac gcctaacatg cgctcaatct gcttgcttga catgcctttc ttgctggcgc
     4141 aaagcaggtg cgtcgccaat acccacttgt gcagaggtat cttggagcgc tcgaaaacgg
     4201 tgccgaccgt cacggtaaac tgggtgcggc agtccccgca gaaatacagg ccagaacggt
     4261 ggctggaacc gttcaggagg ctattacgct caatgccgcc gcagtggggg cataccgggc
     4321 cattgggcca gcggatcgct tctaggtgct tacgggccgc ttcgtcgtct ttgaagcgga
     4381 gttcggtgag gatggacttg gccataggtg tctcctaact acggccccat tctccccttc
     4441 tattgtgcgt ttgtcaagta tataatgacg aaagaatcgg caacctggca tgaggtggag
     4501 gtggaggccg ccggccagcg cgtggacaac tacctgacca agcggctgaa aggggtgccg
     4561 aaaagccaca tctaccgcat cctgcgcagc ggtgaagtcc gcgtgaacag tcggcgcgtg
     4621 ggacccgaat accgggtgca ggaaggcgac cgcctgcgcc tgccgccggt gcgcacggcg
     4681 aaacccgccg cggtgccgaa gcaaccgcca tcgactcgtc tcaaggcgaa agttctgtac
     4741 gaggacgacg tcctgctggt catcgacaaa ccggcgggcg tggccgtgca tggcggcagc
     4801 gggatcagcc ggggcgtggt cgagcagttg cgcctagagc ggcctgaagc gcgatttctg
     4861 gaactcgtcc accggctcga ccgggaaacc tcgggcgtgc tgatgctcgc caagcgtcgc
     4921 tccgcgctca ccgagctgca ccggcaactc cgggaagggc gggtgcagaa gtcttacctg
     4981 gctctggtga agggcgcttg gcgcgacggg aagcgcagca tcaagctgcc gctgcacaag
     5041 tacgtgctgg cttcggggga gcgccgggtg agcgtgagcc gcgacgggct gcccgcccac
     5101 accatattcc ggctgcaacg aagctggagc ggctacagtc tgctcgaggc cgagctcaag
     5161 accggccgta cccaccagat ccgcgtgcat ctcgcacacg tcggctatcc ggtggcgggg
     5221 gacgataagt acggcgattt cgcgctcaac aagcagctcg cccggcaagg gctgaagcgg
     5281 atgttcctgc atgcgttcaa gatcgtaatc gcccatcccc aaagcggtgc ggtgctcacg
     5341 ctggaggcgc cgctgccgcc cgatctgcag gcgtttctcg cggcgctcaa cctcgaggaa
     5401 gcgcgtcttg cccaaaaact ttgatctgct ggtgttcgac tgggacggca cactgatgga
     5461 ttcggccgct gccatcacgg cctcgctcca ggcggcatgc cgcgacctgg aactcccggt
     5521 accaaacgag gaacgggccc gctacgtcat cggcctcggg ctcaatgacg caatggccca
     5581 tatcctgccc ggcctggatc cggcacgcta cccggaggtg gtggcgcgct accgcgtcca
     5641 tttcctggag cgcgactcgg acactgcgtt gtttcccgga gcggccccgg tggtggtggc
     5701 gctccacggg gccggttttc tgctggcagt ggcgacgggc aagagccgcc gcggcctgga
     5761 acgggcactc gccgccaccg aacttgagcg gtacttccac gccacgcgct gcgccgacga
     5821 ggggcacgcc aagccccatc cgggcatgct gctggggctg atggaacagc tgggcgtcac
     5881 ggccgggcgg acgctgatga taggggacac gacgcacgac atggagatgg cccgcgccgc
     5941 gggcgtgccc cgtctcggtg cggcgtatgg cgcgcactcg aaggaagcat tgctcggtta
     6001 cggaccggtc gcctgcgtgg acgatttcga gggcctgcgg caatggctga tgcgaaacgc
     6061 gtgatatctg catggcgggg ccgggatgcc gctaaaatac cgtttcaccg cggcctgcgc
     6121 tggcggcggc gaccctgact gaggatggca atcatggcag aactggaacc aggcggaacc
     6181 ggaccgagct gggaacgcca ggcgatcgag aaggtcgcgc tggcagcgat cgaggagcag
     6241 cgccgctcgc ggcggtgggg catcttcttc aagttgctgc tgttcatcta tctcttcgcg
     6301 ctgctgtttg tcggcatggg ctggatcggc aagagggagg tcagtcccgg caagcacact
     6361 gcgctggtcg aggtgcaggg tgtcatttcc tccaacagcc ccgccagcgc ggaaaacatc
     6421 actaccgggc tgcaggatgc gttcaaggac aagcgcaccc agggtgtggt cctgcgcatc
     6481 aacagcccgg gcggcagccc ggtgcaggcg ggacacatca acgatgaaat ccggcggttg
     6541 cgcgccaagt atcccgacat cccgctctac gcggtggtcg aggacgtctg cgcctcgggc
     6601 ggctattaca tcgcggtggc cgccgaccgg atctacgtgg acaaggcgag catcatcggc
     6661 tcgatcgggg tgctgatgga cggcttcggc ttcaccggca cgatggagaa gctcggcgtc
     6721 gagcgccggc tgctcgcggc gggtgagaac aagggcttcc tcgacccgtt ttcgccgata
     6781 ctcgagtcgc agaagcagca cgccgagcag atgctgaacc agatccacca gcagttcatc
     6841 accgtggtgc ggcagggccg cggcaagcgg ctgaaagaaa cgccagaaat gttttccggc
     6901 ttgctgtggg tcggccagaa aagcgtcgag ctcggtctcg ccgacgcgct cggcagcgtc
     6961 gactacgtgg cgcgcgaggt gatcaaggct gaggacatcg tggactttac cccgcgcgag
     7021 aacgtcgccg agcggctcgc aaggagattc ggggccggca tggccgacgg cctggcgcac
     7081 ttcggcgtct tccaggggat gacgctgcgc taggattttg ccgcagcagc gcccgcaaaa
     7141 ttctagccgc gctcgcgtag caccaggaac accaccagcc gccggtcgag atccggcggc
     7201 ttttttttcc attccgcgac gggacgagtc gtcacggatt cggttggcag cgtgaggtcg
     7261 gccgcaatgc agagcagcgt gtcgccgcgg cagtgctcca gcagcgcatt cagcatggcg
     7321 ctgctgcggt acggcgtctc gatgaagatc tgggtctcgc cccgggcccg cgtctcgatg
     7381 tccttgagct tgcgtgcgcg ttcagccctg tccacgggta gatagccgtg gaatgtgaag
     7441 tgctggccgt ccatgccgga agccatcagc gccagcagaa tagacgacgg tccgaccagc
     7501 ggcaccaccc gtacgccgtg ttcgtgcgcg cgccgcacca gcgccgcgcc ggggtcggcc
     7561 accgccgggc agcctgactc cgacatcagc ccgcagtttt cacctttcag caatagcgcg
     7621 agcagggatt cgacgtcatc ctcgttcgcg tgttcgtcga aaaggtgaaa actgagttcc
     7681 tgcagtgggc gaggatgatg gaccgccttc aagaagcgcc gcgccgattt ggcgttttcg
     7741 acgataaaag tgcgcaggct gcgggtgaca tcgagcgtcg cggagggcag attggatagc
     7801 gcgtcttcgc ggcccagcgc aaccgggatc aggtagagcg ttccgttgcc cgcgcgcggt
     7861 tcagacaatg gcgacgcctt cttttctgag catggtggca agccgcatca gcggcaggcc
     7921 gatcaaggcg ctgggatcgt cgccgacgac gcgctcgacc agcacgatgc cgagcccctc
     7981 gatcctggcg ctgcccgcgc agtcataggg ctgttccagc tcgagatagc gttcgatctc
     8041 gcggtcgctg tagttgcgga agtagacggt ggtcggcaca tccgccgtct gcatgccgcc
     8101 gctttcagtg ttgagcagcg ccagcgcggt gtggaatatc acgcgcttgc cgcgcatggc
     8161 cttgagctgc gccagcgcgt tttcgtagct gccgggcttg ctgaggcagg cgccgtcgag
     8221 cgccgccacc tggtcgcagc cgatcaccag cgctttcgac gatgacggcg ccgccgccag
     8281 cgccttggcc tgcgcgagcc tcagggcggt gtcgcgcgcg gcctcacccg gcagggcggt
     8341 ctcgtccacg gcaggcgctt tcacctcgaa cggcagccgc agccgcatca acaaggcgcg
     8401 gcggtacggc gaactcgaag cgagcacaat gcgtggggaa tccaagcgcc ggctggttgc
     8461 taagtccctg ccagattagg atttttcttc aaaatctctt tgacagagcg gtgcaaaaat
     8521 actatcatgc gccgcctatg actgtcattg acagtctgga attcgcccgg actggacaga
     8581 cgctgagtgg aagcttgccg gtcccggcgc tgacgcggtt gcgggacagc ctgttcgacg
     8641 acctcggcga ggtccggttc gaggtaaagg gcgctcacga tgcgcgccgc cggccgatcc
     8701 tgatgctcga aatcagcggc gtgttgcacc tgcagtgcca gcgctgcctg ggaatgctgg
     8761 attacccgtt gcggattacc aataccctgc tgctggccgg tgcgggcgcg gccgccgcgg
     8821 gaagcctcga cgaggaagat ttggaatgga tcgaggcgaa cgtggatctc gacgtggcga
     8881 gcctcgtaga ggatgaagtc ctactgagtc ttccctatgc gccgcggcac gcggaagggt
     8941 tgtgccggca cggcaggagc ggggaggccg gggatgccgc agcgtcggcg tttgcgaagc
     9001 tcgccgcgtt gaaacggaac aataattgat actcaaggag cgatagacat ggcagtccag
     9061 cagaaccgga agtccccttc gaagcgcggc atgcaccgct cgcacgacgc gctgtccagc
     9121 ccgccgctgg cactcgagcc gaccaccggc gaggtgcatc tgcggcacca catcagcccg
     9181 aacggctatt accgcggcaa gaaggtcgtc aagaccaggg gcgaataggc gtttgcccgg
     9241 ccccatacga tcacggacgg cgcgccgctc tgcggcgatg acacgcgccg tggaggaggg
     9301 ttgcggatga ttgtgaaggt tgccatcgat tgcatgggtg gcgatcacgg cgcgcacgtc
     9361 accgtgcccg ccgcgctggg cgttctccgc gacgatccgg agatctcgtt cctcctggtg
     9421 ggccggcagg atgcgatcga gcgcgagctg gtgcgctgcg gtcccgcgcc ggaggggcgg
     9481 acgagcgtgc ggcacgcgag ccaggtggtc gaaatggacg aggcgccggt ggccgccatg
     9541 cgcggcaaga aggattcctc gatgcgcgtc gccatcaacc tcgtcgagca gggcgaggcg
     9601 gcggcctgcg tcagcgcggg caataccggc gcgctgatgg cggtttcgcg tttcgtgctg
     9661 gggacgcttc ccggcatcga ccgccccgcg atcgccacga tgctgccgac gctgaagggc
     9721 catacttaca tcctggacct cggcgccaac gtcaactgtt cgccggagca cctgctgcag
     9781 ttcggcatca tgtgttcgga gctggtgagc tcgatcgaag gcaagccgcg ccccaccgtg
     9841 ggactgctca acatcggggt ggaggacatc aagggcaacg agctggtgaa gcaggccgcg
     9901 gagctgatgc gcacgagcga cctcaacttc cacggctacg tcgagggcga cgatatctac
     9961 aagggcacga ccgacatcgt ggtgtgcgac ggctttgtcg gcaacgtggc gctgaagacc
    10021 tccgagggac tggcgcagat gctggccacc tacctgcgcg aggaattcgg gcgcaatccg
    10081 ttcacccggc tcgccgggct ggtggcgctg cccgtgatca gcgccttcaa gcatcgcgtg
    10141 gatcaccgcc gctacaacgg tgcgagcctg ctggggctgc gcggcatcgt ggtcaagagc
    10201 cacggctcgg ccgacgcgtt cgccttcggg ttcgccatcc ggcgcgcgat cgaggaggcg
    10261 cggaccggcg tcctcgcgca catcagccaa cgcatggcgg ccatccacaa gagcgcggcg
    10321 tgagcagcta ttccaggatc attggcaccg gcggctatct cccggccaag gtgctcgcca
    10381 accatgacct cgagcggatg gtggatacca ccgatgagtg gatctacacg cgcaccggca
    10441 tccggcaaag gcacgtcgcg gcggatgacg agaagacgag cgaccttgcg ctgcacgcga
    10501 gccgcagggc cctggaagcg gccggtgtcg cggtcgcgga cgttgatctc atcgtggtgg
    10561 cgacgaccac gcccgacatg gtatttccga gcacggcgtg catcctgcaa tccaagctcg
    10621 gcgttcgcaa ctgtccggca ttcgacgtgc aggcggtatg cagcggcttt atctacgggc
    10681 tcgccacggc cgatcagttc ctgcgttccg ggcagtatcg cagcgccgtc gtggtgggcg
    10741 ccgagatcta ttcccacatc ctcgactgga aggatcgctc gacctgcgtg ctgttcgggg
    10801 acggcgccgg cgcggtcgtg ctccggcgcg acaatgcgcc tggcatacta tcgagccacc
    10861 tgcatgccga cggttcgtat gcgaacatgc tgtccgtgcc gggaacggtc agcggcggca
    10921 gggtgagcgg caggccactg ctgcagatgg acgggggagg ggtgttcaag tttgcggtca
    10981 aggcgctcga tgacctggtc gaggaggcgc tggcggcgaa cggcatgcag aaagccgaca
    11041 tcgactggct ggtcccgcac caggccaata tccggatcat ccaggcctcg gcgaagaagc
    11101 tcggcatgag catggagaaa gtggtggtga cggtggaccg ccacgcgaac acctcagccg
    11161 cctcgatccc gcttgcgctc gacgaagcgg tgcgcgacgg gcgcatccgg tccggccagc
    11221 atgtgctgtt ggaagccgta ggcggcggct ttacctgggg cgcggtgttg gccaggtggt
    11281 aaacacggct catcggtcac gggtcacgga tcatgaaatt cgcattcgta tttccaggcc
    11341 agggctcgca gtccgtcggc atgatgcagc ccttctcgga atcgaaaggc gcgcgcgcag
    11401 tgttcaccga agcttccgat gtgctcaagc aggatttgtg ggagctggtg gccgaagggc
    11461 ccgcggatga gctcaattcc acagtcaaca cgcagcccgt gatgctcacg gcgggttacg
    11521 cggtctaccg tgcctggcgc gatgcgggcg gagcggaacc ggcgctggtc gccggccaca
    11581 gcctgggcga atacaccgca ctggttgccg ccggggcgct ggcgttccgc gatgctcttc
    11641 cgctggtccg cttccgcgca caggcgatgc aggaagccgt gccggtgggc gcgggcgcga
    11701 tggccgccat cctcgggctg gaggatgacg cggtgcacgc ggcgtgcagc gaggcggcgc
    11761 aaggacaggt ggtcgagccg gtcaacttca atgcgccgag ccaggtcgtg atcgccggtc
    11821 acaaggcggc agtagagcgc ggcgtggcgg cggcaaaggt gaagggggcc aggcgcgcgg
    11881 tgttgctgcc ggtgagcgcg ccttttcatt cctccttgct caggccggct gcagataagc
    11941 tggcggtcta cctgggcaag gtcggcgtga gcgcgccgcg catcccggtg gtaaataacg
    12001 tggatgtcgc gacggaaagc caacctcaac gcatcaagga cgccctcgcg cgccaagcgt
    12061 gcaacccggt acgctgggtc gaagtggtgc gccgaatcgc cggctcgggc atcgcgcacg
    12121 tcgcggaatg cggccccggc aaggtgctgg cgggactgac cagacgcata aacggcaatc
    12181 tccagggtta cgcgatcgcc gatccgcaat cgctggagca ggcgttgcag gcattcaaat
    12241 cgtgagcctc gacggaaaaa tcgcgctcat caccggcgcc tcgcgcggca tcggccaggc
    12301 gatcgcgctg gagctgggca ggcacggcgc cgtcgtcgtc ggcacttcga ccacgcaagc
    12361 cggcgcggac ggcctccagc agcggctctc ggcggcgggc atccagggcg cgggcatggc
    12421 gctcaacgtc aacgacgcgc ggcagtccga agctgtcatc gcggaaatcc agaaacggtt
    12481 cggtgacatc acgatcctcg tcaacaatgc cggcgttacg cgcgataacc tgctggtgcg
    12541 catgaaggac gaggagtggg acgagatcat ggcgaccgac ctcaaatcgg tctaccggct
    12601 ctcgaaactg gtgttgcgct cgatgatgaa ggcgcgctac gggcgcatca tcaatatcac
    12661 ctcggtggtc ggcgtcaccg gcaatgccgg ccagaccaac tacgccgccg ccaaggccgg
    12721 catcatcgga ttttccaagt cgctcgcgcg cgaagtgggg agccggaaca tcaccgtcaa
    12781 ctgcatcgcg ccgggcttca tcgagaccga tatgacgcgg gcgctcaacg agcagcagcg
    12841 cggcgccttg atcgagagca tcccgctggg ccggctgggc cgccccgagg acgtagcggc
    12901 agccgtggct ttcctggtct cgcccggggc ggattacatg accggatgca cgttgcacgt
    12961 gaatggcggc atgcatatgg actaggcata ccgccatccc tcacccccgc ccctctcccg
    13021 cgccgcgggc gaggggggtt cgagtcactc gcgttgatcg cgagcgatga aataggacgg
    13081 gcggcatgta catggattaa acaacacttt gcgttgaaat tcagaggctt gttttactgg
    13141 cgaatctggt aaaatagcgc cctgattcaa caccgaccgc cccggtcaca gcctggcttc
    13201 ggggccattc acgagcatca acatcctgtc gaggaaaggg gacctgaatg gagaacatcg
    13261 agcatcgcgt caagaaaata gttgccgagc agctgggcgt caacgaggcg gacgtcaaga
    13321 acgaatcttc tttcgtcaac gacctgggcg cggattcgct cgacaccgtg gagctggtga
    13381 tggcgcttga ggaggaattc gagaccgaga tccccgacga ggaagcggag aagatcgcca
    13441 ccgtccagca agccatcgac tacatcaagg cccacgtcaa gtcctgattc ccctcgcagt
    13501 ccggagcgaa tttgtccaga cgcagagtcg tcgtcaccgg tctcggcatc gtttccccgg
    13561 tcggaaccgg ggcgcctgaa gcctggaaaa acatcctcgc cggccaatcc ggcatcaccc
    13621 gcatcacccg tttcgaccct tcggccttca gctcgcagat cgcgggcgag gtgaagggct
    13681 tcgacccggc gcaatacctg tcggcaaagg aagcgcgccg cttcgacacg ttcatccact
    13741 acggcctggc ggcctcgatc gaggcgatca aggacgcggg actcgatttc gagcgcgaga
    13801 gccgcgagca ggtcggtgca tgcatcggct cgggcatcgg cgggctgccg ctgatcgagg
    13861 agatgcacaa tgcctttctc gccggtggcc cgcgcaagat atcgccgttc ttcgtgccgg
    13921 gcacgatcat caacctgatc gcgggccagc tctcgatcat gtacggcctc aaaggtccga
    13981 atctggcgct ggtgaccgcg tgcacgaccg ccaaccattg catcggggac gccggccgcc
    14041 tgatcgagta cggcgacgcc gatgtgatga tcgcgggcgg gtccgaagcc tgcgtgtcgc
    14101 cgctcggcgt gggcggattc tgcgcggcgc gcgcgctgtc ctcgcgcaac gacgaccccg
    14161 ccgccgccag tcgcccgtgg gaccgggacc gcgacggctt cgtgctgggg gagggcgccg
    14221 gtatcctggt tctcgaggaa tacgggcacg ccagcaagcg gggcgcgagg atttactgcg
    14281 agctcgccgg ctacggcatg agcgcggatg cgcaccatat caccgccccg tgcgaggacg
    14341 gcgagggcgc cgtgcgctgc atggccaacg cggtgaggaa cgcgggcatc aaccgcgacg
    14401 aggtggatta catcaacgcc cacggcacgt cgaccccgct gggcgacgtt gccgagacgg
    14461 tggcggtgaa ggattgtttc ggcggtcacg cgaagaagct ggccgtgagc tcgaccaagt
    14521 ccatgaccgg gcatttgctg ggcgcggccg gcggcgtcga agcggtgttc accgcgctcg
    14581 cggtgcgcga ccaggtcgcg ccgccgacca tcaatcttgt caacccggac ccgcaatgcg
    14641 acctggacta cgtccccaac gcggcgcgca agatgaagat cggcgtcgcc atttcgaact
    14701 cgttcggttt cggcggcacc aacggcagcc tggttttccg caggatttga cccccgctcg
    14761 gggcctgggc gcattgccat gattccggga atgcggcgac cggagctggg aggccggagc
    14821 ttttaccgcg cgcgtgagaa aatggctcga tgaagaaagg gcttgagcgt tttctgcggg
    14881 tcctgctgag cgcgtcggtt gcgggcatcg tcatcgcctc cggcgctttc acgtattacg
    14941 ccacgaccga gatcgatgtc ggcccgtcgc caatccagtt caacctgaag acgggaagca
    15001 gcctgcgcag cgcggcgcag cagatggaac gggcgggcgt gctgcgccac cccggcattt
    15061 tcaccgtgat ggcccggctg ctcggcgaag cgggcaacat caaagccggc atctacgaga
    15121 tcagcggtcc ggtcacgccg taccaactgc tgctcaaggt cacggaaggc gatgtcacgc
    15181 aatccacgat taccctcgta gagggctgga ccttccggca gatgcgcaag ctgctcgacg
    15241 accatcacgc ggtgaagcac gatacgcgcg ggctgccgga tgcggaagtg ctgcgacgcc
    15301 ttgcgatcga tgcggcttcg cccgagggac tgttcttccc cgatacctat catttcaatg
    15361 tcggcgccag cgactttctc atcctgaaga gcgcctatcg cctgatgcag tcgcatctca
    15421 aatcgcagtg ggcgggccgc gcccccgatc tgccattggc ttcaccctat gaggctttga
    15481 tactggcttc catcgtggaa aaggaaaccg gcagagttga agaccgtcct ctgatcgccg
    15541 cggtattcgt caaccggctg cgcaagggca tgctgctgca agccgatccc accgtgatct
    15601 acggcatggg agaggcgttc gacggcaacc tgcgcaagca cgacctggcg atggatacgc
    15661 cgtacaacac ctatacccgc gcaggcctgc cgccgacgcc gatcgccatg ccggggctga
    15721 cctcgctcac cgtcacgctc aatccgccca ggagcgacgt gctgtatttc gtgtcgaggg
    15781 gagacgggtc ctcccatttc tcgcgcacgc tcggtgagca cgagcgcgcc gtgacaaaat
    15841 atcaacgatt aggacgacgt tgatgcgcgg aaaattcatc acgctcgaag gtctggacgg
    15901 ggcgggcaag agcacccacc tgcgctggat cgcgcgcttt ctcaggggcc gcggcatcca
    15961 ggtgaagctg acacgggagc cggggggcac tgccgcgggg gagaagctgc gccggctgct
    16021 gctcggtcgc agacaggaac tacatccgga aaccgagacg ctgctcatgt tcgcggcgcg
    16081 gcgcgagcac ctggacaagg tgatcatccc ggcgctgaat gccggccggt gggtgctgtg
    16141 cgaccgcttt accgatgcca cctatgcata tcaatcgggc ggcagcggcg tgggctggaa
    16201 caaggttgcc gagctcgagc aatgggtgca ggcggacttg cagcctgatc tcaccctcat
    16261 tttcgatgtc agcccggagg tcgggcggca acgggcgggc cggcaaagaa gaaagcccga
    16321 tcgtttcgag cgcgaacagg acaagtacca ccagcgcgtg cgtgaagcct acctgagacg
    16381 ggcccgtgaa aacgctgaac gggtgcacgt ggttgacgcg aacggcagcc ttcccgaagt
    16441 tcataaagag cttgaacgac tgatctcctc cttttgcaaa tgatgtctta ttcctggcac
    16501 aaacctgacg gcgatgcctt gctcaggcgc aaggatcgat tgcctcacgc gctgttgctg
    16561 cgcggtcctc agggcatcgg caagctggct ttcgccgaag cgctggcgcg agccctgtta
    16621 tgcgaacggc cggagccgga cggttcggct tgtgggcatt gcacggcctg cgggtggatg
    16681 gagcagggcg cccatccgga tctccggcgc ctcgagccgg aaggcctggc cgaacaggac
    16741 gaggcggagc agggcgggga gaagaaggcc agcgtgcaaa tccaggtgga gcaggtgcgg
    16801 ggaatcggcg gcttcatcaa catctccagt caccgcggtg gcgtaaaggt cgtgctgatc
    16861 catcccgcgg aaacgctcaa catcaacgcc gccaatgccc tgcttaaaaa cctggaggag
    16921 ccgccgccgc gcacgtactt cctgctggtg gcgcatcgct ggcatcaact gccgcccacg
    16981 atcagaagcc gttgccagca catcgcattg ccgccgccgc cggcggagac ggcgcgcagc
    17041 tggctcaagc agcagggact tcgggatccc gatctggcat tggcgcaggc gggaggcgcg
    17101 ccgctgctgg cgctgaaatt cgacgaggag tactggcgcg agcgcgagct gttcatcaag
    17161 gcggtcgccg ataacgggtt cgatgccctg cgtctcgccg agcaattccg tggccaggag
    17221 ccagcgctga tcgtgagctg gctgcagaaa tggtcgttcg atctggtttg ccacaagctg
    17281 ac
//
LOCUS       DBNGWZ010000233         4760 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2417455, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000233 DBNGWZ010000000
VERSION     DBNGWZ010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2417455"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16CX_07030"
     CDS             complement(<1..719)
                     /locus_tag="AC16CX_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3012304.1"
                     /translation="MSSDFLSGLNEQQLEAVTLLRQSALVLAGAGSGKTRVLTTRVAW
                     LIQTGQASPHGLIAVTFTNKAAKEMLTRISAMLPINTRGMWVGTFHGLCNRMLRAHYR
                     EANLPQLFQILDTQDQLSLIKRLLKTLNVDEERFPPRQVQWFVTANKEEGQRAARVEA
                     HDEFTRRMKDIYAAYDEQCNREGVVDFAELLLRSYELLSSHSALRDHYRARFRHVLVD
                     EFQDTNRLQYRWLKLLAQTTPA"
     gene            complement(959..1837)
                     /locus_tag="AC16CX_07035"
     CDS             complement(959..1837)
                     /locus_tag="AC16CX_07035"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3012305.1"
                     /translation="MKFLYAFGALVALFPVSATAQTLTVEGVASPAWLERGGSRMPLA
                     VGMRLADRDRIVTGTGARALLRMAEGSAVKLGENAVLAVDGLTDRKGPDVGRLITASL
                     DVARGAFRFTTDIFGKRRASRDVKIRITTITAGIRGTDVWGKSDQERDLVCLIEGRIA
                     VNHPQAGEFTMADPLSFFVAPRKAQPLPIAPVDPRQLKQWAAETEIAPGSGAARRGGR
                     YHVDASAAPDQKTALAEYDKLLAAGYPALIRPVKKEDGSLEYRVRIENLPTRQDAGAL
                     VKKLKALGMAGAVISN"
     gene            1931..4582
                     /gene="leuS"
                     /locus_tag="AC16CX_07040"
     CDS             1931..4582
                     /gene="leuS"
                     /locus_tag="AC16CX_07040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3012306.1"
                     /translation="MQQKYNPHAIERDAQRHWESAGTFRATETPGKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLTRYYRMKGYNVLQPMGWDAFGLPAENAAMANNVPPARWTY
                     DNIVYMKLQLKSLGFAIDWTRELATCRPDYYRWNQWLFLRMLEKGLVYKKTGVVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPMYYMAITNYAEELLAALERLPGWPERVKT
                     MQANWIGKSYGVRFAFPYTLDGKPEKLWVFTTRADTIMGVTFCAVAAEHPLAAYAAKS
                     NPELAAFVEECKRGSVMEADLATLEKKGMPTGLSVTHPLTDEPVPVWVGNYVLMAYGE
                     GAVMGVPGHDQRDFEFATQYQLPIPAVIKPKDGELKLPLEQAYVEYGMCFNSGKYDGF
                     DYRQAVDAIAADLKAKGLGDKQVLWRLRDWGISRQRYWGTPIPIIHCSRCGDVPVPDA
                     DLPVVLPEDCVPDGTGNPLAKRADFVNCSCPKCGKPAKRETDTMDTFVDSSWYYVRYA
                     CPDNANSMADGRVAYWLPVDQYIGGIEHAILHLLYSRFWTKVMRDLGLVKFDEPFSNL
                     LTQGMVLNEIYLRKGESGRITYYNPAEVEATTDEAGNRSAVLKADGKPVEFDGIGTMS
                     KSKNNGVDPQELIDHYGADIARFFMMFTSPPEDTLVWSDAGVEGAARYLRRLWMFVYD
                     RREWIEAESGRRDKQRSQYSYPADLAKAAPDLAAVRREIHAILKQANYDFDKFQFNTV
                     ASAGMKIFNALSSLPQHHDVRPGSDYAASYQQLLHEGLSILLRLLSPITPHVSHHLWR
                     ELKFGEDVQTAPWPEPDPAALIEDEIELVVQVNGRKRGDVRVPRDADSGTIERIVLAD
                     PAVQRHVSGQPVKKVVVVPGRLVNLVV"
BASE COUNT          874 a         1518 c         1545 g          823 t
ORIGIN      
        1 gccggggtgg tttgggccag cagcttcagc cagcggtact gcaggcggtt ggtatcctgg
       61 aactcgtcca ccaggacgtg gcggaagcgc gcgcgatagt gatccctcag cgccgagtgg
      121 ctcgaaagca gctcgtacga gcgcagcagc agctcggcga aatccaccac gccttcccgg
      181 ttgcactgct cgtcgtaggc ggcgtagatg tccttcatgc gccgtgtgaa ctcatcgtgc
      241 gcttccaccc gggccgcgcg ctggccctcc tctttgttcg ccgtcacgaa ccactgcacc
      301 tggcgcggcg ggaagcgctc ttcgtccacg ttgagggttt tcagcaggcg cttgatgagc
      361 gagagctggt cctgcgtgtc gaggatctgg aagagctggg ggaggttcgc ttcccggtaa
      421 tgcgcgcgca gcatgcggtt gcacaaaccg tggaaagtgc cgacccacat gccgcgggtg
      481 ttgatcggca gcatcgcgga aatgcgcgtc agcatctcct tcgccgcctt gttggtgaag
      541 gtgacggcga tcaacccgtg cgggctcgcc tgcccggtct ggatcagcca cgcgacgcgc
      601 gtggtgagca cgcgcgtctt gccgctgccg gcgccggcga gaaccagcgc ggactgacgg
      661 agcagcgtga ccgcttcgag ctgctgttcg ttgagtccgg aaagaaaatc agaggacatg
      721 aatcggcgtg acggatcgcg ccgattataa atgagttaaa ccggaagcaa agccccggtt
      781 taactagcgc gtcggaacgt tgagcagagg ccccatttat ggggatgcga ccgtgaagac
      841 gcgcaagcgg ccgatgcgag atcgttgact gcttgccgct agcaggacag aggccgccgc
      901 gtcggaacgg acgagagtgg tcgccgttca acgcgcggcg tgcggtcttc cgatagccct
      961 agttgctgat gaccgcgccc gccattccga gcgccttcaa ctttttcact agcgcaccgg
     1021 catcctgccg cgtcggcagg ttctcgatgc gcacgcggta ttccagggaa ccatcctcct
     1081 tcttcaccgg ccgtatcagc gcagggtagc ccgccgcgag cagcttgtcg tactcggcga
     1141 gcgccgtctt ctgatcgggc gcggcactcg cgtccacatg gtagcgtccc ccgcggcgcg
     1201 cggccccgct gcccggcgcg atctcggttt cagcggccca ctgcttcagc tgccgggggt
     1261 ccaccggggc gatcggcagc ggctgcgcct tgcgcggcgc gacgaagaag gacagcgggt
     1321 ccgccatggt gaactcgccc gcctgcggat ggttgacggc gatcctgccc tcgatcaggc
     1381 agacgaggtc gcgctcctgg tccgattttc cccacacgtc ggtgccacgg atgccggcgg
     1441 tgatggtggt gatccggatc ttgacgtcgc ggctcgccct cctcttgccg aagatgtcgg
     1501 tggtgaagcg gaatgcgccg cgcgcgacat cgagcgaggc cgtgatcagc cggcctacgt
     1561 ccgggccttt cctgtcggtg agaccgtcca ccgcgagcac ggcgttctcg ccgagtttca
     1621 ccgcgctgcc ttccgccatg cgcagcagcg cgcgcgcgcc ggtgccggtg acgatccggt
     1681 ccctgtcggc gagccgcatg ccgacagcca gcggcatacg cgagccgccg cgctccaacc
     1741 atgccggact cgccacgcct tcgacggtga gcgtctgcgc ggtggcggac acagggaaca
     1801 gcgccaccag cgcgccgaaa gcatacagaa acttcatgat tttctcccct gctgtctgcc
     1861 gatcccgtcc tatacggcca agtaccgatg ccagatgcag ctataatcgt ggctttattc
     1921 acgcacttcc atgcagcaga aatataaccc gcacgcgatc gagcgtgacg cccagcggca
     1981 ctgggagagc gcggggacct tccgcgcgac ggagacaccg ggcaagccca agtactactg
     2041 cctctcgatg ttcccctacc cctcgggcaa gctccacatg gggcacgtgc gcaattacac
     2101 cataggcgac gtgctgaccc gttactaccg gatgaagggc tacaacgtgc tgcagccgat
     2161 gggctgggat gcgttcgggc tgcccgccga gaacgcggca atggcgaaca acgtcccgcc
     2221 ggcaagatgg acctacgaca acatcgtcta catgaaactg cagttgaagt cgctcggctt
     2281 cgccatcgac tggacgcgcg agctcgccac ctgcaggccc gactattacc gctggaacca
     2341 gtggctgttc ctgcgcatgc tcgagaaggg tctcgtctac aagaagaccg gcgtggtgaa
     2401 ctgggacccg gtggaccaga ccgtgctcgc caacgagcag gtgatcgacg ggcgcggctg
     2461 gcgcaccggg gcgctcgtgg aaaaacgcga gatcccgatg tactacatgg cgatcacgaa
     2521 ctacgccgaa gagctgctcg ccgcgctcga gcggctgccc ggctggccgg agcgcgtgaa
     2581 gacgatgcag gccaactgga tcggcaagag ctatggcgtg cggtttgcgt ttccctatac
     2641 gctcgacggc aagccggaga agctctgggt ctttaccacc cgcgccgaca ccatcatggg
     2701 cgtcactttc tgcgcggtcg ccgccgagca cccgctcgcg gcgtacgccg cgaagagcaa
     2761 tccggagctc gccgccttcg tcgaggaatg caagcgcggc agcgtgatgg aagccgatct
     2821 cgccaccctg gaaaagaagg gcatgccgac ggggctgtcc gtcacgcatc cgctcacgga
     2881 tgaaccggtg ccggtatggg tcggcaacta cgtgctgatg gcctacggcg agggcgcggt
     2941 gatgggcgtg ccggggcacg atcagcgcga cttcgagttt gcaacgcaat accaattgcc
     3001 gatcccggcg gtgatcaagc cgaaggacgg ggagctgaaa cttccgctcg agcaggcgta
     3061 cgtcgaatac ggcatgtgct tcaattccgg caaatacgac ggcttcgatt accggcaggc
     3121 agtggacgcg atcgccgccg atctcaaggc gaagggcctg ggcgacaagc aggtgctgtg
     3181 gcggctgcgc gactggggca tctcgcgcca gcgctactgg ggaacgccga ttccgatcat
     3241 ccactgttcc aggtgcggcg acgtgccggt gccggatgcg gacctgccgg tggtactgcc
     3301 ggaggactgc gtgccggacg gcacgggcaa cccgctcgcg aagcgcgccg actttgtcaa
     3361 ctgctcctgc ccgaagtgcg gcaagccggc caaacgcgaa accgacacga tggatacgtt
     3421 cgtggattca tcgtggtact acgtgcgcta tgcctgcccc gacaacgcga acagcatggc
     3481 ggacgggcgc gtcgcatact ggctgccggt ggaccagtac atcggcggca tcgagcacgc
     3541 catcctgcac ctgctctact cgcgcttctg gaccaaggtc atgcgtgacc tcggcctggt
     3601 gaaattcgac gagccgttct cgaacctgct cacccagggc atggtgctga acgagatcta
     3661 cttacggaag ggcgaaagcg gccgcatcac ctattacaac ccggccgagg tcgaggcaac
     3721 gacggacgag gccgggaacc gcagcgcggt cctcaaggcc gatggcaagc cggtcgaatt
     3781 cgacggcatc ggcaccatgt cgaagtccaa gaacaacggc gtggacccgc aggagctgat
     3841 cgaccactac ggcgccgaca tcgcgcgttt cttcatgatg ttcacctcgc ccccggaaga
     3901 cacgctggtg tggagcgacg ccggcgtcga gggcgcggcg cgctacctgc ggcggttgtg
     3961 gatgtttgtc tacgaccgac gcgaatggat cgaggcagag tcaggccgtc gagacaaaca
     4021 acgatcgcag tattcctatc cggcggatct cgccaaggcc gcgccggatc tggctgccgt
     4081 gcggcgggaa attcacgcta ttctgaaaca ggcaaattac gacttcgaca aattccaatt
     4141 caacacggtg gcgtcggcag gcatgaaaat cttcaacgcg ttgagcagcc tgccacaaca
     4201 tcacgacgtg cgtcccggtt ccgattatgc ggcgtcttat caacagctct tgcacgaagg
     4261 tcttagcatc ctgctgcggc tgctctcgcc catcacgccg cacgtctcgc accacctgtg
     4321 gcgcgagctg aagttcgggg aggacgtcca gaccgcgcct tggccagaac ccgatcccgc
     4381 ggccctgatc gaggacgaga tcgagctggt ggtgcaggta aacggcagga agcgcgggga
     4441 cgtgcgcgtg ccgcgcgacg cggacagcgg aacgatcgag cgcatcgtgc tcgccgatcc
     4501 ggcggttcag aggcacgtca gcggccagcc ggtcaagaag gtcgtcgtcg tgcccggacg
     4561 gctggtcaat ctggtagtct gagggcctgt gaagtcgaaa gtcgaaagtc ggaaggcgga
     4621 agtaactgcg cagctcaggc ggtttgcaca acgcctttcc tttcgccttt cgccttccgc
     4681 ctttctcctt gccgcgaccg cattgctggt cgcggcgtgc ggcttccagc ttcgcgggac
     4741 ggtgtcactg cccttcaact
//
LOCUS       DBNGWZ010000234         6148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_24415, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000234 DBNGWZ010000000
VERSION     DBNGWZ010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_24415"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="AC16CX_07045"
     CDS             <1..1007
                     /locus_tag="AC16CX_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012307.1"
                     /translation="VAIYRQIYLTAMVIPVISVLGVVFAFFLRRRAIQRLVAQGMDPV
                     KAREAFAGPGDRPAPNWWILGGSLLFVAFTLGMGFSNFAYNQEVIFAGSFAIIAFLIA
                     KLTRELEPEARATLIGTAIVIFIYRAVPASGEGLTWWMIDELKFDQRFLAVLSLIASV
                     LTLFGMFLFRRFMAVRSIAYVVGFLTIAGTLLSAPIVGMYYGLHHWTAAVTGGVVDAR
                     FIVLVNTALESPLGQIAMIPMLAWIANSAPQNLKATYFAIMASFTNLALSAAQLGTKY
                     LNQIYTVTREVKDPVSGAVQVAADYSELGVLLIAQVLIGLALPFAAIFFARITRLRSA
                     "
     gene            1271..3445
                     /locus_tag="AC16CX_07050"
     CDS             1271..3445
                     /locus_tag="AC16CX_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC26A/SulP transporter family protein"
                     /protein_id="HIR3012308.1"
                     /translation="MTGDLWGGLAAMLVALPSSIAFGVLIYTAIGPEHAGEGALAGIL
                     GAAALGIVAPLVGRNGGFNTAPCAPAAAVLSALALELAEGDHTGERIAMLLAVTALLS
                     AIFQVLYGVVRAGRLIKFIPYQVVSGYLSGVALIIAVGQLPKLLGLPKDVPLGRGLIS
                     PELWNESGIVVGAVTILAMAAAPRFIQKIPAAIIGLIAGIAAYFALALFDPGLLKVAG
                     NPLVIGPIEATGSFVGTVGSRFSSLLAVRPDDIELIFVSALTLSVLLSIDTLKTGVVL
                     DALARRRHDSNRELIGQGAANAAAVFVGGMPGAGTMGATLVNFTSGGRSPWSGVAEGA
                     FVVAAFVLLGGLIAWVPIGALAGLLLVVAWRMFDREMFRLLLRPSTRVDFGVIAAVVI
                     VAQVGLIAASVTGIVLAILLFIRDQIRGSVIMSKVDLRGVHSKRRRLADERELLDQHG
                     RQAMVVQLHGNLFFGTTDQFFSELEKDLTTLRYLLVDLRHVQSMDFTAAHLIEQMKER
                     LAERGGELLFSGMPSSLPTRQDIEDYMAQLGLVRKGGGIRVFDTRDSAIEWMEGKILE
                     AAGWTPQDEGPPLALGQIELFEELEPEAIAELGDAVYEITVAAGGRICSAGDHGDEMF
                     LVRRGRVHALLPLEGGKRHHLATFCPGDFFGELAFLDRQPRSADAEAATAASLYALSR
                     ARFDELTRKSPALGGKIFEQLAFAISKRLRAADTELRMLEER"
     gene            3547..>6148
                     /locus_tag="AC16CX_07055"
     CDS             3547..>6148
                     /locus_tag="AC16CX_07055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000716 - transcription-coupled
                     nucleotide-excision repair, DNA damage recognition
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3012309.1"
                     /translation="MLNLDVPPPGMRVRTPPLYGSSDALMLAQLARAQRPLAVVTAAG
                     ADAQRLLEEIPFFGKDLRVHLLPDWETLPYDQLSPHQDLVSERLATLYQLMQRAFDVA
                     IVPVTTALLRLAPVEYLAARSFHFKLGEKLPADALRRQLVTAGYTHATQVVAPGEYCV
                     RGGLIDLFPTGSALPYRIELDDDVVESIRSFDVDSQRSIYKVNEVRLLPAREFPLDEE
                     GQTRFRRNFRERFEGDPSRSRIYKDISKGVPCAGIEYYLPLFFERTALLTDYLPQETL
                     LCVQPDLQQAIEQFWSDTHARYAMLRGDRAHPVLPPHDLFQTADEFFGAIKPFARVEL
                     SSAGESRLGATKPEEGVETHPSSLIPPPSTLDGHPSSFILHPSQAETAATQPLPPIAV
                     ERRADDPLHRLRSFAATFRGRVLVLAESPGRRETMLEYFSEHGLQPAAAQDYARFATS
                     SEPFMLGVGPLHAGFVTADPPLAIVTENELYAHQVRPRRDRDGAQRATPEGMLRDLSE
                     VRVGDPVVHESHGIGRYLGLVTMNLGDGETEFLTLEYAGGDKLYVPVSSLYLVSRYSG
                     APAEAAPLHQLGSGQWDKAKRKAARQVRDTAAELLALYAQRAAREGHAFRVKDHDYEA
                     FADGFPFEETPDQAAAIKATLSDLTTGRPMDRLICGDVGFGKTEVALRAAFVAVADGK
                     QVAVLVPTTLLAEQHHNTFSDRFADWPVKLAELSRFRTGRETAEALAGLASGRIDITI
                     GTHKLLSRDVKFGNLGLVIIDEEHRFGVRQKERLKALRAEVDVLTLTATPIPRTLAMS
                     LEGLRDFSVIATAPERRLAIKTFVARHSRPLIREAALRELKRGGLVYFLHNDIDTIHH
                     VEASLVKLLPE"
BASE COUNT          953 a         2108 c         2016 g         1071 t
ORIGIN      
        1 aggtggcgat ctaccggcag atctatctca cggcgatggt aatccccgtc atttccgttc
       61 tgggcgtcgt attcgcgttt ttcctcaggc gcagggcgat ccagcgtctc gttgcgcagg
      121 gtatggatcc cgtcaaagcc cgggaagcct tcgccgggcc cggggaccgc cctgcgccta
      181 actggtggat tctcggcggg agcctcttgt tcgtggcatt cacgctcggt atgggcttca
      241 gcaacttcgc ctacaaccag gaggtgatct tcgcaggctc cttcgccatc atcgcgtttc
      301 tcatcgccaa gctcacccgc gagctggagc ccgaagcgcg cgcgacgctg atcggaaccg
      361 ccatcgtgat tttcatctac cgcgccgttc cggcgagcgg cgagggcctg acctggtgga
      421 tgatcgacga gctgaaattc gaccagcggt tcctcgccgt gctctcgctc atcgccagcg
      481 tgttgacgct gttcggcatg ttcctgttcc gccgcttcat ggccgtgcgc tcgatcgcgt
      541 atgtggtggg ctttctgacg atcgccggca cactgctatc ggcccccatc gtcgggatgt
      601 attacggcct gcaccactgg accgccgccg tcacgggcgg cgtggtggat gcgcgcttca
      661 tcgtgctggt gaacaccgcc ctcgagtcgc cgctcggcca gatcgcaatg attcccatgc
      721 tcgcctggat cgccaattcg gccccgcaaa acctcaaggc cacctacttc gcgatcatgg
      781 cctccttcac caacctcgcc ctgtcggccg cgcagctcgg gaccaaatac ctgaaccaga
      841 tctacaccgt tacgcgcgag gtgaaggacc cggtgagcgg cgcggtacag gtggcggcgg
      901 attacagcga actcggcgtg ctgctgatcg cgcaagtgct gatcggcctt gcgctgccgt
      961 tcgcggccat cttcttcgcg cggatcaccc gcttgcgcag cgcgtaggaa tttgtcgggc
     1021 tcagcctcga caaattcctg atgcaaaacc gcccacatgg aaacagataa atattcagcc
     1081 cgcatgctcc cacgcccgtt caccgcccaa ggccttcaaa atccgtcgtg ggctggccat
     1141 tcgcgaaaca tgcgggctcg ggctggcgca acgatacaat ccagtccatg atgactggat
     1201 ctgagggcaa gccagcgtga gcggcagcgc acaacccgtg acaggcagaa cccacgccgg
     1261 cgacacgagc ttgacaggtg atctgtgggg cggccttgcc gcgatgctgg ttgcgctccc
     1321 ctcgtcgatc gcgttcggag tcctcatcta taccgcgatc gggccggagc acgcggggga
     1381 aggcgcgctc gccggcatcc tcggcgcggc ggcgctcggc attgtggcgc cgctcgtcgg
     1441 gcgcaacggc ggcttcaaca ccgcgccgtg cgcgcccgcg gcggcggtgc tctcggcgct
     1501 cgccctcgag ctcgcggaag gcgaccacac gggagagcgc atcgcgatgc tgctcgcggt
     1561 gaccgcgctg ctttcggcga tcttccaggt gctctacgga gtcgtgcgcg ccgggcgcct
     1621 gatcaagttc atcccgtacc aggtggtgag cggctatctg tccggcgtgg cgttgatcat
     1681 cgcggtcggc cagctgccga agctgctcgg cttgcccaag gacgtcccgc tcggccgcgg
     1741 gctcatctcg ccggaactct ggaacgaaag cgggatcgtg gtcggcgcgg tgaccatcct
     1801 cgcaatggcg gccgcgccgc gcttcatcca gaaaattccc gcggccatca tcgggctcat
     1861 tgccgggatc gccgcttact tcgcgctcgc gctgttcgat ccgggtctcc tgaaggtcgc
     1921 cggcaacccg ctcgtgatcg ggccgatcga ggcgaccggt tcgttcgtcg ggactgtcgg
     1981 ctcgcgcttt tcgtcgctgc tcgcggtgcg gccggacgat atcgagctga tcttcgtttc
     2041 cgcgctgacg ctctcggtgc tgctctcgat cgacacgctg aagacgggcg tcgtgctgga
     2101 cgcgctcgcg cgccggcgcc acgattcgaa ccgcgagctg atcgggcagg gcgcggcgaa
     2161 cgccgcggcg gtgttcgtgg gcggcatgcc gggggcggga acaatgggcg ctaccctcgt
     2221 caatttcacg agcggcggca ggtcgccgtg gtcgggcgtc gccgaaggag cgttcgtcgt
     2281 ggccgccttc gtgctgctcg gcgggctcat cgcctgggtg ccgatcgggg cgctcgcggg
     2341 gctcctgctc gtcgtggcgt ggcgcatgtt cgaccgcgag atgttccgcc tcctgctgcg
     2401 gccgtccacg cgggtggact tcggggtgat tgccgcggtc gtgatcgtcg cgcaagtggg
     2461 gctgatcgcc gcctccgtga cgggcatcgt cctggcgatc ctgctgttca tccgcgacca
     2521 gattcgcggc tcggtgatca tgagcaaggt cgatctgcgc ggggtgcact ccaagcgccg
     2581 gcgcctcgcc gacgagcgcg agctgctcga ccagcacggt cggcaggcga tggtggtgca
     2641 gctgcacggc aacctcttct tcgggacgac cgaccagttc ttctcggagc tggagaagga
     2701 cctcacgacg ctgcgttacc tgctggtcga cctgcgtcac gtgcagtcga tggacttcac
     2761 cgccgcgcac ctcatcgagc agatgaagga gcggctcgcg gagcgcggcg gcgagctgct
     2821 gttcagcggc atgccctcga gccttcccac ccgccaggac atcgaggact acatggctca
     2881 gctcgggctc gtgcgcaagg gcggcggcat ccgggtgttc gacacgcgcg acagcgcaat
     2941 cgagtggatg gagggaaaga tcctcgaggc cgccggctgg acgccgcaag acgaagggcc
     3001 gccgctcgcc ctcgggcaga tcgagctctt cgaagaactc gagcccgagg caatcgccga
     3061 gctcggcgac gcagtatacg agatcacggt cgcggcgggg gggcgaatct gctcggccgg
     3121 cgaccatggc gacgagatgt tcctggtgcg ccgcgggcgg gtgcacgcgc tgctgccgct
     3181 cgaaggcggc aagcgccacc atctcgccac cttctgcccg ggggacttct tcggcgagct
     3241 tgcgttcctc gaccggcagc cgcgctcggc ggacgccgaa gccgcgaccg cggcaagcct
     3301 ctacgcgctc tcgcgcgcgc gcttcgacga gctcacgcgt aagagccccg cgctcggcgg
     3361 caagatcttc gagcagctcg cgttcgcgat ctcgaagcgc ctgcgcgcgg cggacacgga
     3421 gctgcggatg ctcgaggagc ggtgaagcga cgtctgccct cctatccgac caccagccgg
     3481 ttcaggagct gcggctgggc cacggcgtag ccgcaacagc ctgtataatc acgcgttttc
     3541 tccgacatgc tgaaccttga cgttccgccg ccggggatgc gcgtacgtac gccacccctg
     3601 tacggctcct ccgacgcgct gatgctcgcg caactcgcgc gcgcgcagcg cccgctcgcc
     3661 gtcgtcacgg ccgcgggggc ggacgcgcag cgcctgctcg aggaaatacc ttttttcgga
     3721 aaggatctcc gggtccatct cctgcccgac tgggaaacgc tgccgtacga ccagctgtcg
     3781 ccccaccagg acctggtgtc cgaacggctc gcgacgctct accagctgat gcagcgcgcg
     3841 ttcgacgtcg cgatcgtgcc ggtcacgacg gcgctcctgc gcctcgctcc ggtcgagtac
     3901 ctcgccgcgc gctcgtttca cttcaagctg ggggagaaat tgccggccga tgctttgcgc
     3961 cgccagctcg tcacggccgg ctacacgcac gcgacgcaag tggtggcgcc gggcgagtac
     4021 tgcgtgcgcg gggggttgat cgacctgttc cccacgggca gcgcgcttcc ctaccgcatc
     4081 gagctcgacg acgacgtggt cgagagcatc cgcagtttcg acgtggacag ccagcgctcg
     4141 atctacaagg tgaacgaagt gcggctgctg cccgcgcgcg agttcccgct ggatgaggag
     4201 gggcagacgc ggttccgccg caatttccgc gaacgattcg agggcgatcc ctcgcgcagc
     4261 cgcatctaca aggacatttc caagggcgtg ccctgcgccg gcatcgaata ctacctgccg
     4321 ctgttcttcg agaggaccgc gcttctcacc gattacctgc cgcaggaaac gctcttgtgc
     4381 gtgcagccgg atctgcagca ggcgatcgag cagttctgga gcgacaccca tgcgcgttac
     4441 gccatgctgc gcggcgaccg cgcccatccc gtcctgccgc cgcacgacct gttccagacc
     4501 gccgacgaat tcttcggcgc gatcaagcca ttcgcccgcg ttgagttgtc gtccgcgggc
     4561 gaatcacggc ttggcgcaac caagccggag gaaggtgttg agactcatcc ctcatccctc
     4621 atccctccgc cctcaaccct cgacggtcat ccttcatcct tcatccttca tccttcgcaa
     4681 gcggagacgg ctgcgacgca gcctcttcct ccgattgcgg tcgagcgccg cgccgacgat
     4741 ccgcttcatc ggctgcggtc gttcgcagcg acgttccgcg gccgcgtttt ggtgctcgcg
     4801 gaaagccccg gccggcgcga aaccatgctc gagtacttca gcgaacacgg cttgcagccg
     4861 gcggccgcgc aggactatgc gcggttcgcg acctcaagcg agccgttcat gctcggcgtc
     4921 ggcccgctgc acgcgggctt cgtcaccgcc gatccgccgc tcgcgatcgt caccgaaaac
     4981 gagctctacg cccaccaggt ccggccgcgg cgcgatcgcg acggcgctca gcgcgctaca
     5041 ccggaaggca tgctgcgcga cctgtccgag gtcagggtcg gcgatccggt ggtgcacgaa
     5101 agccacggca tcggccgcta cctcgggctc gtcacgatga atctcgggga cggcgagacc
     5161 gagttcctca cgctcgagta cgcgggcggc gacaagctct acgtgccggt ttcgagcctg
     5221 tacctggttt cccgctacag cggcgcgccg gccgaggcgg cgccgctgca ccagctcggc
     5281 agcgggcagt gggataaggc gaagagaaag gcggccaggc aggtccgcga cacggcggcg
     5341 gagctgctcg ctctctacgc gcagcgcgcg gcgcgcgaag gccacgcgtt cagggtcaag
     5401 gaccacgact atgaagcttt tgccgacggt tttccgttcg aagagacgcc cgaccaggcc
     5461 gcagcgatca aggcgacact ctccgatctg accaccggga gaccgatgga ccgcctgatc
     5521 tgcggcgacg tgggcttcgg caagacggag gtggcgctgc gcgccgcgtt cgtcgccgtg
     5581 gccgacggca agcaggtcgc ggtgctggtg ccgaccacgt tgctcgccga gcagcaccac
     5641 aacacctttt ccgaccgctt cgccgactgg ccggtgaagc tcgcggagct ctcgcgcttc
     5701 cggaccggac gggaaacagc cgaagcgctc gccggcctgg cctccggccg catcgatatc
     5761 acgatcggca cgcacaagct gctttcacgc gacgtgaagt tcgggaacct cggcctcgtc
     5821 attatcgacg aggaacaccg tttcggcgtg cggcagaaag agcgcctgaa ggcgctgcgc
     5881 gcggaggtgg acgtgctcac cctcacggcg accccgatcc cgaggacgct cgcgatgtcg
     5941 ctcgaaggct tgcgcgattt ctcggtgatc gcgaccgcgc ccgagcgccg gctcgcgatc
     6001 aagaccttcg tcgcgcgcca cagcaggccg ctcatccgcg aggccgcgct gcgggagctg
     6061 aagcgcggcg gcctggttta cttcctgcac aacgacatcg acacgatcca tcacgtcgag
     6121 gcgtcgctcg tgaagctgct gccggagg
//
LOCUS       DBNGWZ010000235         8059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3424656, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000235 DBNGWZ010000000
VERSION     DBNGWZ010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3424656"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1235)
                     /locus_tag="AC16CX_07060"
                     /pseudo
     CDS             complement(267..1235)
                     /locus_tag="AC16CX_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            complement(1259..2233)
                     /locus_tag="AC16CX_07065"
     CDS             complement(1259..2233)
                     /locus_tag="AC16CX_07065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012310.1"
                     /translation="MKRSMIGAVICAVLCLAQFPALLAQADWPRKPIRVVVPFVPGSF
                     TDVAARAMAAEMTRQFGQTVLVDNRTGAGGTIGTETVARAAPDGHTLLFFTNSLTMAP
                     GLYAKLPYDPVKDFIHVSLVAESPSMLLLRAQAPARTLRELVDLARTRPGDLTYGSAG
                     QGSSAHLAMELFLNIAGIRMLHVPFKGVATSIAEVAAGRIDCSIASLASGMAPVSAGR
                     IQGLGVTGRARSTLLPGVPTFAEAGFPSYDMSYWWAIAAPAGTAPRIVSRLHREIARA
                     ADNPKLKELFLNQGALAATSSPAELALRVGEEVKVWKDVITKAGVKLQ"
     gene            2584..3363
                     /locus_tag="AC16CX_07070"
     CDS             2584..3363
                     /locus_tag="AC16CX_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3012311.1"
                     /translation="MNSLEKMLSLLDVFTPAAPAWSTDELIRYSGSSRSTCYRYINVL
                     QRAGFLTPVANGSYILGPRIIELDRHIRMCDPVYTAGGRPMQQLAKRTGHSALLCMLY
                     SDTVMCVREELNADAPEGLFSRGQKRSLFAGAASKVILAHLPAHQLRSLFAKHRKTIA
                     AAGLGADWESFRKALRKIRQDGYCITTGEFTAGITGIAAPLFNRMEKVLGSLGIAMSA
                     STADRSQFPALIKAVVKAARDVTDQIRSGKHGVDLPARAVG"
     gene            complement(3424..4392)
                     /locus_tag="AC16CX_07075"
     CDS             complement(3424..4392)
                     /locus_tag="AC16CX_07075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3012312.1"
                     /translation="MPKGVRIHALGGPEVLRYEDIAVPAPSGGELRLRHRAIGLNYID
                     IYHRTGIYPIKLPAVIGSEAVGVVEAVGGGVVEFRPGDRVTYAPVIGAYCEIRNLPAE
                     RAIPVPAGISDDEVAGMMLKGMTARSLLRRAYPVKKGDTVLVHAAAGGVGLILSQWAK
                     SLGATVLGTVGSEEKAALAAANGCDHPILYRKADFVAAVKDITNGEGVQAVFDGVGKD
                     TFLKSIECLAPFGTIVSYGQASGRVEPFDIVALREKAAYATRASLAIHVTKRADLLAS
                     ANDLFDVVRRGVVRIAVRRRYALSDAIHAHRDLEGRITDGSSVLIP"
     gene            complement(4402..5415)
                     /locus_tag="AC16CX_07080"
     CDS             complement(4402..5415)
                     /locus_tag="AC16CX_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012313.1"
                     /translation="MKANRKRYPSVLLSFSLCVVMTSGIQDAHAAAPAGESAQAYPSK
                     PIRIVVGFTAGGVADIAARIVATKLAENWKQQVVVDNRPGAGSAIGTGIVASAEPNGY
                     TMLSVSAAHAIIPAVYSKLPFDSARDFAGVAVTATGPLLLIASSKVGVKSVKELIALA
                     KSRPGQLNFASAGVGSATHFAMEVFKYKAGIDIVHVPYKGIPEATTDTMTGRVQLFIS
                     PLINAMPQVKDGKVVALGVTTTFRSALLPDVPTIAEAGLPGYRAESWQGLLVPAKTPR
                     PIVAKLHAEITRILQEPEARQRIAALGLVSPPTTPEEFDKFIRGEITTYQKIARAGNI
                     KAE"
     gene            complement(5513..5923)
                     /locus_tag="AC16CX_07085"
     CDS             complement(5513..5923)
                     /locus_tag="AC16CX_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012314.1"
                     /translation="MQSNRKKSRATKPIAAEVREDVEFMWKQFPGHFDGAYSKVLVGP
                     GTPGARFLDFRISSYEPKAYVKSHAHESKEQIYYFLEGEGLLELGSKKHVVRPKSFAF
                     IPPRLPHALYNTGVFNLVFFVITTSTDNRPGSAG"
     gene            complement(5932..6912)
                     /locus_tag="AC16CX_07090"
     CDS             complement(5932..6912)
                     /locus_tag="AC16CX_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012315.1"
                     /translation="MKRRFLHLLGSTLLAGLASVAGPLIAQDFPNRPVRMIVPYGPGG
                     ITDITARIIAPGMLEDLGQQVVVENRAGGAAIPGFDLVAKSKPDGYTIVAATTALAAQ
                     PILFRRLPYQADRDFAPVSLVITVPTVLVVHPSVPARNVKELIALAKARPGALNYGSA
                     GNGTDNHLTTEVFKHAAKIDALHVPYKGGGAVMLDLVAGQVAFVFSTIPTAHPFISTG
                     RLRALAVSSLKRNAALPEVPTVAEAALPGFNVNAWLGLFGPAGMPATATDRLHAATVK
                     TLQRPEVSKRLAAMGAEIVGSTPAQMASHLKSEMDRWVRLAREVKFEVVD"
     gene            complement(6989..7933)
                     /locus_tag="AC16CX_07095"
     CDS             complement(6989..7933)
                     /locus_tag="AC16CX_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012316.1"
                     /translation="MAAVSLIVAGPAAAQDYPERPIRMVLPYGTGGNADVVSRILSPF
                     MSADLSQQVVIDNRGGGAGIPAMAFVAKANPDGYTALFAASNLASNPILFKKLPYNAE
                     ADFAPVSLIAIVPTVLVVHPSLPVQSVKQLIALAKAHPGELNYGSVGYGSGNHLTTEV
                     FINAAGLKVEHVPYKAAAAFMTDLMAGRLTFVFATIPAAHSHIASKRVRALAVSSQKR
                     SAALPGVPTVSEAAIPGFEVNSWLGIFLPAGTPAKVVDRLNAATTKALQNPKVKEVLA
                     SAGAEPVGGTPAQLGAHLKIELARWEKLARTVTFKVAD"
BASE COUNT         1400 a         2553 c         2637 g         1469 t
ORIGIN      
        1 cggacgatga tcgccgaggc gagggcgctg ggcgggtcgc tgccggcggc ggagaccgcg
       61 ctcaagtgct tcgaagaggc ggcgcgcagc gggctcgggg agaaggatca gtccgcgctg
      121 ccagcgaatt gggcgaagcg ccagaatcgg tgacgaagtg acgcagtgac gcggtcacga
      181 gaaaaaagct cgaatcacga tccacgagtc acgacccacg ccttccagca acaagggcca
      241 gctgatgctg ggctttcttg catagttcat tccagcttga ccccggctcg ggcgatgacg
      301 tccttccaag tctggatttc cgcgttgacg cggcgggtat ggtcggcggg tgagctcgtc
      361 accgcgacgg cgccctgttt ctggaacacg tccttcagcg atggcttttc cgcggcgcgc
      421 gcgatttcct ggttcagccg ggtcacgact tcgcgtggcg ttccgacggg cgccgcgatg
      481 ccccaccagt acgacatgtc gtaacgcggg aaaccggctt cggtgaacgt cggcacgccc
      541 ggcagtatcg gatggcgtta cttgccggat atggcgtagc cccgcgtccg ttcgttgtgg
      601 atgtgcggcg ccccggcggc caggcttgag atcaagacgt cgatgcggtc cgccacggct
      661 tccgccaacg ccggcccgat gcccttgaat ggaacgtgca gcatcttgat cccggccacg
      721 ttgaggaaca gttccatcgc gagatgggcc gaggagccct ggccgccgga gccgaaggtt
      781 agctttcccg ggttggcgcg ggcgagactc accagctcct ggagcgtttt tgccggcagt
      841 ttgatccgtc ccgacatcaa tgaaggcgat tccgcgatct ggctgacgtg gatgaagtcc
      901 ttcagcgggt cgtagggcag tttggcatac aggcccggag agatcgcgaa ggagttgtct
      961 gaaagcagca gggtgtagcc gtcaggcgtc gcgcgggctg ccatttcggt gccgatcgtg
     1021 ccgccggccc cggtacggtt ctcgacgatg acttgttgtc cgagttggcc tgtaagctcg
     1081 acagccagcg tgcgcgcgga cacatcggtg aacgccccgg gttggaaagg cacgatgatg
     1141 cggatcggcc tggccggcca ggcctgcgta gtttgtgcgt gcgataccac ggacagcgaa
     1201 gaaaaagcca aggcaaggac ccatatcctg atcatcggag tctccagtta tcgggggttc
     1261 attgcagttt gacgccggct ttggtgatca cgtctttcca caccttgacc tcctcgccga
     1321 cccggagggc gagttcggct ggcgagctgg tagccgccag cgcgccctgg ttcagaaaga
     1381 gttccttgag cttggggttg tcggcggcgc gcgcgatttc ccggtgcagc cgggacacga
     1441 tgcgcggagc cgtcccggcg ggcgcggcga tggcccacca gtacgacatg tcatagctgg
     1501 ggaaccccgc ctctgcgaat gtcggcaccc cgggcagcag ggtgctgcgc gccctgccgg
     1561 tcacgccaag cccctggatc ctgccggcac tgaccggcgc catgccgctg gcgaggctcg
     1621 cgatcgaaca atcaatgcgt cccgccgcga cctcggcgat cgaagtcgcg acgcccttga
     1681 acgggacgtg gagcatcctg atcccggcaa tgttgaggaa cagctccatc gcgagatggg
     1741 ctgaagagcc ctggcccgcg gagccgtacg tgaggtcccc gggccgggtg cgggcgagat
     1801 ccaccagctc acggagagtc ctcgcgggtg cctgcgcgcg caggagcagc atcgagggcg
     1861 actcggcgac caggctcacg tggatgaaat ccttcaccgg gtcgtagggc agcttcgcgt
     1921 acaagccggg ggccatcgtc agcgagttgg tgaaaaatag cagcgtatga ccgtcgggcg
     1981 cggcgcgtgc taccgtttcc gttccgatcg tcccgccagc gccggtgcgg ttgtccacga
     2041 ggacggtctg gccgaactgc cgggtcatct cggccgccat ggcgcgcgcg gcgacatcgg
     2101 tgaaggaacc aggcacgaag ggaacgacga cgcggattgg cttgcgtggc cagtcggcct
     2161 gcgcaagcag cgcggggaac tgcgcaagac acaggacagc gcagatgacc gccccgatca
     2221 tcgaccgctt cattctcgtg tctcgacctc gacacccgga tggcgcccgg cgaaaccttc
     2281 gagcagcccc gcgcagcact ctcgttctgc agctgagtag acgactaacc actgttcggc
     2341 tgccgcgcgc ccttggttct cgacatggag tggccaacgc agccgttgcc gacagcgtgc
     2401 tgcgcccttt ggatacgtga acaaatggta gccccgtgtc ataataatgt ccagaatggt
     2461 gggatacttt ttaaccatgg acgcacaccg ggcgccgggc gcaagcgggg ggggaccgtg
     2521 cccaagcgct tagagtccac ccgtggacga cgtgccggcg ccgctgttcc gcatggcagg
     2581 tccatgaaca gcctcgagaa gatgctgagc ctgctggacg tgttcacgcc cgccgcgccg
     2641 gcgtggtcca ccgatgaact gatccggtat tcggggtcct cgcgctcgac ctgctatcgt
     2701 tacatcaacg tgctgcagcg cgccggtttc ctgacacccg ttgccaacgg atcctatatc
     2761 cttggaccgc gcatcatcga gctggaccgg cacatccgga tgtgcgatcc cgtgtacacg
     2821 gccggcggcc gtcccatgca gcagcttgca aagcggaccg gccattccgc gctcctgtgc
     2881 atgctctaca gcgacacggt gatgtgcgtg cgcgaggaac tcaatgcgga tgcgccggag
     2941 ggccttttca gccggggcca gaaacgctcg ttgttcgccg gtgcggcctc caaggtgatt
     3001 ctcgcgcacc tgccggccca ccagctgcgc agcctgttcg cgaagcaccg caagaccatt
     3061 gctgctgccg gcctcggcgc cgactgggaa agcttcagaa aggcgctgcg caagattcgc
     3121 caggacggct actgcatcac gaccggggaa ttcacggcgg ggatcaccgg catcgcggcg
     3181 cccctgttca atcgcatgga aaaagtgctt ggcagcctcg gcattgccat gtccgcgtcc
     3241 actgccgacc gttcgcaatt cccggcgctc atcaaggcag tcgtgaaagc cgcgcgcgac
     3301 gtgaccgatc agattcgctc gggcaaacac ggcgtcgacc tgcccgcgcg ggcggtgggg
     3361 tgacggctcc gagaggcgtg atccagcgac gggtatccgg acgcgcccgc gtccgccccc
     3421 gcatcacgga atcagaacgg acgatccgtc cgtgatccgc ccctcgagat ctcgatgggc
     3481 atggattgcg tccgagaggg cgtaacggcg ccgtaccgcg atcctgacga ctccacgccg
     3541 gaccacatcg aacaagtcgt tcgccgacgc gagcagatca gctcgtttcg tgacgtgaat
     3601 cgcgagcgac gcccgcgtgg catacgccgc cttttcccgc agcgccacga tgtcgaaggg
     3661 ctcgacgcga cctgacgcct gtccgtaact gacgatcgtg ccgaaagggg cgaggcactc
     3721 gatcgacttc agaaacgtgt ccttgccgac gccgtcgaat accgcctgta cgccttcgcc
     3781 gttcgtgatg tccttgactg ctgcgacgaa gtccgccttc cggtaaagaa tagggtgatc
     3841 acatccattg gcggcggcca gagccgcttt ctcctcgctc ccgaccgtgc cgagcacggt
     3901 tgctcccagg cttttcgccc actgggacag gatgaggccg acgccgccag cggccgcgtg
     3961 cacgagcacc gtatcgccct tcttgaccgg gtacgctcga cgcagcagcg agcgggccgt
     4021 catgcctttc agcatcatcc cggccacctc gtcatcgctg atgccggcgg gcacgggtat
     4081 ggctcgttcc gccggcaggt tgcggatttc gcaataggcg ccgatcaccg gagcgtaggt
     4141 gacgcgatcg cccggcctga attcgacaac gccgccgccg accgcttcga cgacgccgac
     4201 ggcctcgctg ccgatgaccg ccggcaactt gatcggatag atcccggtgc ggtgataaat
     4261 gtcgatgtag ttcaacccga ttgcccggtg ccggagccgc agctcgcccc ctgatggggc
     4321 gggcacggcg atgtcctcat atcgcaagac ttcgggaccg ccgagcgcgt gaatgcggac
     4381 gcctttgggc atggcgattt ctcattccgc cttgatgttg cccgcgcgcg cgatcttctg
     4441 gtaggtagtg atctcgccgc ggatgaactt gtcgaactcc tccggcgtgg tgggcgggct
     4501 caccaggccc agcgccgcga tgcgctggcg cgcctccggt tcctggagga tgcgcgtgat
     4561 ttccgcgtgc agcttggcga cgatcgggcg cggcgtcttc gcgggcacga gcaggccctg
     4621 ccaggattct gcgcgatacc cgggtagccc tgcctcggcg atcgtcggca cgtcggggag
     4681 cagggcgctg cggaacgtgg tggtgacgcc gagcgccacc actttgccgt ccttgacctg
     4741 cggcatggcg ttgatcagcg gcgagatgaa caattgcacg cggccggtca tggtgtcggt
     4801 ggtcgcctcg ggtatgccct tgtagggcac gtgcacgatg tcgatgcccg ctttgtactt
     4861 gaacacctcc atcgcaaaat gcgtggcgct gccgacgccc gcggaagcga agttgagctg
     4921 gccgggcctg ctcttcgcca gtgcgatgag ttccttcacc gatttgacgc ctaccttcga
     4981 ggacgcgatc agcaaaaggg gtccggtggc cgtgacggcg acgccggcga aatcccgggc
     5041 gctatcgaac ggcagcttgg aatacacggc gggaatgatc gcgtgcgccg cggaaaccga
     5101 aagcatcgtg taaccgttgg gttcggcgct ggcgacgatc ccggtcccga tcgcgctgcc
     5161 ggcgccggga cggttgtcca ccaccacctg ctgcttccag ttctcggcca gcttcgttgc
     5221 gacgatgcgg gccgcgatgt ccgccacgcc gcccgcggta aagccgacga cgatgcggat
     5281 cggcttgctc ggataagctt gtgccgactc cccagcggga gctgctgcgt gcgcgtcctg
     5341 aattccgctt gtcatcacga cgcacagaga aaagctgagc aaaacgctgg ggtatcgctt
     5401 ccggtttgct ttcattgtgc ctcctcgctg tcccgaagct cgcgtcgccg tacccctcga
     5461 acggagcaac cctcggggcc gcccgcaccg gtcggtggga aaatcccgct tcctacccgg
     5521 cgctgcccgg tcggttgtcg gtggacgtcg tgatgacgaa aaacaccagg ttgaacaccc
     5581 ctgtgttata gagcgcgtgg ggcagcctag gcgggatgaa cgcaaagctc ttcggccgca
     5641 cgacgtgctt tttcgatccc agctcgagca gcccttcacc ctcgagaaaa tagtagatct
     5701 gctccttcga ttcgtgcgcg tggcttttca cgtacgcctt gggctcgtag ctcgagatgc
     5761 ggaagtcgag gaagcgtgcg cccggcgtac ccggaccaac caggaccttc gaataggcgc
     5821 cgtcgaagtg gccggggaac tgcttccaca tgaactccac gtcttccctg acttcggccg
     5881 cgatcggctt ggttgcgcgt gacttcttgc gattgctttg catattccac ctcaatccac
     5941 cacttcgaat ttcacttccc tggccagcct gacccagcga tccatctcgc tcttcaggtg
     6001 gctcgccatt tgcgcgggcg tgctgccgac gatttcggcg cccatcgccg cgagccgttt
     6061 cgaaacctcg ggacgctgca atgtctttac cgtcgccgcg tgaagacggt ccgttgccgt
     6121 cgccggcatg ccggcggggc cgaacagtcc gagccaggcg ttgacattga accccggcag
     6181 cgcggcctca gccacggtcg gcacttcggg cagggctgca ttgcgcttca ggctgctgac
     6241 cgccagggcc ctgagccgcc cggtcgagat gaaaggatgg gcagttggaa tcgtggagaa
     6301 cacgaacgcg acctggccag cgacgagatc gagcatgacg gcgccccctc ccttgtacgg
     6361 gacgtgcagg gcatcgattt tggcggcatg cttgaacacc tccgtggtga gatgattgtc
     6421 ggtgccgttt ccggcggaac cgtagttcag cgcacccggc ctggcctttg cgagcgcgat
     6481 aagctctttt acgttacggg ccgggaccga agggtgtacg acgaggacgg tgggtactgt
     6541 gatgaccagg ctgacgggcg cgaaatctct gtctgcctgg tacggcaatc tcctgaaaag
     6601 gatgggttga gccgccagcg ccgtcgtcgc ggcgacgatg gtatagccgt cgggcttcga
     6661 tttcgcgacc aggtcgaagc ccgggatcgc ggcgccgccg gcacggtttt cgacgacgac
     6721 ctgctggccg agatcctcca acatgcccgg cgcgatgatg cgggccgtga tgtcggtgat
     6781 gccgccgggc ccgtagggaa cgatcatccg gaccggtcgg ttggggaaat cctgcgcgat
     6841 tagcgggccg gccaccgatg ccaatcctgc cagcagcgtt gatccgagta agtgaagaaa
     6901 tcgtctcttc atggttttct ccgggtaagc ggggcggtcg ctctctgggt tcaggtcttc
     6961 aggccggacg ttgaccgatg aggtcgcctc agtcggctac tttgaacgtc actgtccggg
     7021 ccagcttttc ccagcgagcc agctcgatct tcaggtgcgc tcccaactgg gccggcgtgc
     7081 cgcccaccgg ctcggcgccc gcggatgcca gcacttcctt caccttggga ttttgcaacg
     7141 ccttggtcgt ggccgcgttc aggcgatcga cgactttcgc cggcgtaccc gccggcagga
     7201 aaatccccag ccacgagttc acctcgaacc cggggatggc ggcctcggat acggttggca
     7261 cgccgggcag cgcggcgctg cgtttctggc tgctgacagc cagggccctt acccgcttgc
     7321 tggcaatgtg cgagtgggcg gcgggtatgg ttgcaaacac gaacgtcagc cgcccggcca
     7381 tcaagtcggt catgaaggct gccgcggcct tgtacggcac atgttcgacc ttcaggccgg
     7441 cggcattgat gaacacttca gtcgtgagat gattgccgga gccgtagccg acagaaccgt
     7501 aattcagctc gccgggatgc gccttcgcga gcgcgatcaa ctgcttcacg gactggaccg
     7561 gaagcgaggg atggaccacc aggacggtcg gcacgatcgc gatcaagctc acgggcgcaa
     7621 aatccgcctc ggcgttgtac ggcagcttct tgaacaggat cggattcgag gcgagattgg
     7681 acgcggcgaa cagagcggtg tagccgtcgg gatttgcttt cgcgacaaaa gccatcgccg
     7741 ggatacccgc accgcctccg cggttgtcga tgacgacctg ctggctcaga tcggccgaca
     7801 tgaacggcga gaggatgcgc gacacgacgt cggcattgcc gccggttcca tagggaagga
     7861 ccatccggat cggacgctcg ggatagtcct gtgcggcggc agggcccgcc acgatcagac
     7921 tgacagcggc caacagcgca gaccggaaaa agtgaatcgg catgttcacg gcgtccgggg
     7981 aagggcgatc ggtgggccgg gctttattga aaatatatta ctaattgaga tatattgtca
     8041 aaaacaacgg ccccgctcc
//
LOCUS       DBNGWZ010000236         6175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2794844, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000236 DBNGWZ010000000
VERSION     DBNGWZ010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2794844"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /gene="amrB"
                     /locus_tag="AC16CX_07100"
     CDS             <1..1376
                     /gene="amrB"
                     /locus_tag="AC16CX_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR3012317.1"
                     /translation="GTARGTVRPPAVAGQFYPNAPAEIRAMINTWLASKRFTGGSPKA
                     VIAPHAGYVYSGPVAASAYDLLRPARGVVRRVVILGPCHRVPVRGLALPQAKAFATPL
                     GRVPLDAEGVSAIRDLPQVVESAATHAEEHALEVQLSFLQRVLGDFSIVPLVVGDVAP
                     EEVAEVLERLWGGAETLIVISSDLSHYHPYDRAREIDGATVRAILDFDHGISHEQACG
                     ATPLAGLLIAARRKGLAPRLLDCRNSGDTAGDKRRVVGYASFALGAESASYGAEHGRT
                     LLRIARSSIAYALGAGSAGSTGANETWLQESRATFVTLMQGEELRGCVGALEARRPLA
                     EDVAANARAAALEDSRFDPLTLEEFARTQIEVSLLSTPKTLAFEDHADLLRRLRPGLD
                     GVILEQLEEGRRATFLPQVWEDLADPEQFIARLKHKAGIAPGTDTRRCRVKRYSVLKW
                     REVELGP"
     gene            1373..2464
                     /gene="amrS"
                     /locus_tag="AC16CX_07105"
     CDS             1373..2464
                     /gene="amrS"
                     /locus_tag="AC16CX_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR3012318.1"
                     /translation="MSAVLDQPYVGRWWHLLEDGRMQCDLCPRDCRLHEGQRGACFVR
                     MREANRMVLTTYGRSSGFCIDPVEKKPLNHFYPGSSILSFGTAGCNLACKFCQNWDIS
                     KSRDMDRLADEASPQAIAEAAAAYGCKSVAFTYNDPVIFAEYAMDIADACHDLGIQAV
                     AVTAGYIHAEPRREFYAKMDAANVDLKAFTDDFYFKLCAAKLQPVLDTLAYLKHETRV
                     WFEITTLLIPGKNDSSAEIEAESKWIMKELGPDVPVHFTAFHPDYKMTDIPATPAATL
                     RRARDIALAEGLQYVYTGNVHDSEGGTTYCPGCKSALIVRDWYRIDDYRVTPDGKCPD
                     CGTAVAGRYERFRHAFGPRRVPVAIHRPA"
     gene            2915..3697
                     /locus_tag="AC16CX_07110"
     CDS             2915..3697
                     /locus_tag="AC16CX_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012319.1"
                     /translation="MRLPISCMECFRVDGHPSDVAISVELRDDGLYTSICPKGHITVT
                     AIQEQKFEVLFDLGAMALLDGYTREAVSSIAASLERFFEYYVLVISLKHGIEYEQFSN
                     AWNPISRLSERQLGAFLFVYLLENKKALEPLILDAKPQFDGRSRGDTPTWSAFRNDVI
                     HKGYIPSEVEVIEYGNLVYQFIYRLIGELKTGAAEFMGKATFHHLARGHSAAGGQQVS
                     TMSVPTLISLVRAQGPAPHLRDALPGLEKYRRWLYARPQSNP"
     gene            3907..4599
                     /locus_tag="AC16CX_07115"
     CDS             3907..4599
                     /locus_tag="AC16CX_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012320.1"
                     /translation="MQNLVDVLELREEVESRGYSAEGMDFESFYDDLVTYGTDPELKR
                     LIEQRTYEYFSSLGLPDTPTIYDYLVLSLRNKDLIASFNWDPFLLQAYRRNGGRGPES
                     LPGVAFLHGNVCIGVCHADKVCGLNGGRCSKCGTNFEPSKLLYPVKQKDYASDPFIAS
                     EWDRLRDHLSRAYFLTIFGYSAPKTDVEARRLMLEVWQENHHLELAEVEIIEPLGSGP
                     YFIVHDIDGAMS"
     gene            4681..4902
                     /locus_tag="AC16CX_07120"
     CDS             4681..4902
                     /locus_tag="AC16CX_07120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3012321.1"
                     /translation="MSDADVAEILKLPPEERLRLVELIWESLAATPSAVPLGDAHRAA
                     IDEELAEHRRNPNDVLTHEELISGVRKPR"
     gene            4899..5207
                     /locus_tag="AC16CX_07125"
     CDS             4899..5207
                     /locus_tag="AC16CX_07125"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3012322.1"
                     /translation="MTLPVVYRRRVQLDLAAAFDWYEEQRTRLGEEFLSAVQSTFQTI
                     ELYPEIFESIHGEVRRAIVPRFPFAVFYIVEPRRTVILRVLHTARNPALWPVPKRQAR
                     "
     gene            5430..>6175
                     /locus_tag="AC16CX_07130"
     CDS             5430..>6175
                     /locus_tag="AC16CX_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012323.1"
                     /translation="MIAVTLRWIDVNILSLGRELRLSYLPPLMVYVAYGISGLTGIVG
                     TFFVKDYLGLSAAFLAALGFWAGIPWVLKMPLGHIVDLIWRWKSWLVFLGAGLIAASL
                     AIMVGLLTDRTAMTAFMPAEAWFVLSVLLSPVGYVVQDVVADAMTVEAVPRVDERGTP
                     VPQEARKLMNTTMQTLGRVALIGGLALVAAINLYLFADVEKLSEAQKVAIYRQIYLTA
                     MVIPVISVLGVVFAFFLRRRAIQRLVAQGM"
BASE COUNT         1174 a         1889 c         1879 g         1233 t
ORIGIN      
        1 ctggaactgc gcgcggcaca gtgcggccgc ccgccgtggc gggacagttc tatccgaacg
       61 ccccggccga gatccgagcc atgatcaaca cgtggctcgc ctccaaacgg ttcactggcg
      121 gctcgccgaa agcggtcatc gcaccccacg ccggctacgt ttactcgggt ccggtggcgg
      181 cgagcgccta cgacctcttg cggcctgcgc gcggcgtcgt caggcgcgtg gtgatcctcg
      241 gtccctgcca tcgcgtgccg gtgcgcggcc tcgccctgcc gcaggcaaag gccttcgcga
      301 ccccgctcgg ccgcgtcccg ctcgacgcgg agggcgtgag cgcgatccgc gatctgccgc
      361 aggtggtcga atcggccgcg acgcacgccg aggagcatgc gctcgaagtg cagctgtctt
      421 tcctgcagcg ggtgctgggg gatttctcga tcgtgcccct ggtcgtcggc gatgttgctc
      481 cggaagaggt ggccgaggtg ctggagcgcc tgtggggcgg ggcggagacg ctgatcgtga
      541 tcagctcgga cctgtcgcac tatcaccctt acgaccgcgc gcgcgaaatc gacggcgcca
      601 ccgtgcgggc gatcctcgat ttcgaccacg ggatctctca cgagcaggcc tgcggcgcga
      661 ccccgctcgc gggcctgctg atcgccgcca ggcgcaaggg tctcgcgccg cggctgctcg
      721 attgccgcaa ctcgggcgat accgccgggg acaagcgccg cgtcgtcggc tacgcgtcct
      781 ttgccctcgg ggccgaaagc gcgagctacg gggcggagca tggaaggacg ctccttcgca
      841 tcgcccgcag ctcgattgcc tatgccttgg gcgccggctc tgccgggtcg accggggcga
      901 acgaaacctg gctgcaggaa tcgcgcgcga ccttcgtcac cctgatgcaa ggggaggagc
      961 tgcgcggctg cgtcggcgcg ctcgaagcgc gaaggccgct cgccgaggac gtcgccgcca
     1021 acgcccgcgc cgccgctctc gaggacagcc gcttcgaccc cctcacgctc gaggaattcg
     1081 cgcgcacgca gatcgaagtg tccctgctgt ccacgcccaa gacgctcgcg ttcgaagacc
     1141 acgccgatct gctgcggcgc ctgcgcccgg gactggacgg ggtgatcctc gagcagctcg
     1201 aggagggcag gcgcgcgacc ttcctgccgc aggtctggga ggatctcgcc gaccccgagc
     1261 agttcatcgc gcgcctgaag cacaaggccg gcatcgcgcc gggcaccgac acgcggcgct
     1321 gccgggtgaa gcgctacagc gtgctcaagt ggcgcgaggt ggagttgggg ccatgagcgc
     1381 cgtcctcgac cagccctacg tcggccgttg gtggcatctg ctggaagacg gcagaatgca
     1441 gtgcgacctt tgtccgcgcg actgcaggct gcacgaaggc cagcgcgggg cgtgcttcgt
     1501 gcgcatgcgc gaagcgaacc ggatggtgct gaccacctac ggccgctcct cgggcttctg
     1561 tatcgatccg gtggagaaaa agccgctcaa tcatttctac ccgggctcga gcatcctgtc
     1621 cttcggcacg gcgggctgca atctcgcctg caagttctgc cagaactggg acatctccaa
     1681 gtcgcgcgac atggaccggc tcgccgacga ggcctcgccc caagcgatcg cggaagcagc
     1741 ggcggcctat ggctgcaaga gcgtcgcctt cacttacaac gatccggtga tcttcgccga
     1801 gtatgcgatg gacatcgcgg acgcctgtca cgacctcggc atccaggccg tcgcggtcac
     1861 tgcgggctac atccacgccg agccgcggcg cgagttctac gccaagatgg acgccgcgaa
     1921 cgtggatttg aaggcgttta ccgatgattt ctattttaag ctgtgtgcgg cgaagctgca
     1981 gccggtcctg gatacgctcg cctatctcaa gcacgaaacg cgcgtctggt tcgaaatcac
     2041 cacgctgctt atccccggca agaacgattc cagcgcggag atcgaggccg agagcaagtg
     2101 gatcatgaag gagctggggc ccgacgtgcc ggtgcatttc accgcgtttc acccggacta
     2161 caagatgacc gacatacccg cgacccccgc cgcgacgctg cgccgcgcgc gcgacatcgc
     2221 gctcgcggaa gggctgcaat acgtttacac cggcaacgtt catgattccg aaggcggcac
     2281 gacttactgc ccaggctgca agtcggcgct gatcgtgcgc gactggtacc gcatcgacga
     2341 ctaccgcgtc acgccggacg ggaagtgccc ggattgcggc acggcagtcg cggggcgcta
     2401 cgagaggttc agacacgcgt tcgggccgcg gcgggtgccg gtggcgatcc accgcccggc
     2461 gtgaaaagca gaaccaccaa gaagatcaag aacaataatt gaaccgctag gcgccaagaa
     2521 aaacaaaatt agaatcgaac cgcagagacg cggagagaag caaaatcaga attaaaccgc
     2581 caaggccgcc aagtagcgtt aatgccggac gcttcgcgcc ggtttaactc cgcgtgatcc
     2641 aacgcacgca ggcagcacca ttcatcacgc ggcagttagc catcaagata gcgtctcgca
     2701 cgtcatcaaa caggcaggtc cttgccacag ccgcacccgc ggggcgagtt tccataattc
     2761 atcgtcggcg agtaaccgac tatttttcgt actcgtgccc gctatcgcat tgatcgggta
     2821 gagggacgac atcgccaagc gcaaaatggc gctttgcaaa tcgcctattt tgtcaaccaa
     2881 ttcgttagat tgagatctcc caccgagagg tgcaatgcgc ttgccgataa gctgcatgga
     2941 gtgttttcgg gtggatggac atccatccga cgtggctatt tcggtggagt tgcgcgatga
     3001 cggcctttac acgtcgatct gccccaaagg tcacattact gtaactgcca ttcaggagca
     3061 gaaatttgaa gtcctattcg atttgggcgc gatggcgttg ttggacggct atacgcgaga
     3121 ggccgtttca agcattgctg cgtcactgga acgcttcttt gaatattacg tgctggtcat
     3181 atcactgaag cacggaatcg aatacgagca gttcagcaat gcgtggaacc cgatctcccg
     3241 gctttctgaa cgccagttgg gtgcctttct tttcgtctac ctgcttgaga acaagaaagc
     3301 actcgaaccg ctcatacttg acgcgaagcc gcagtttgat ggtcgtagtc gaggagatac
     3361 gccgacctgg agcgcattcc ggaacgatgt gattcacaag ggctacatcc cctccgaggt
     3421 agaagtgatc gagtatggaa accttgtcta tcagttcatc taccgcctca tcggcgagct
     3481 gaaaacgggc gcggcagaat tcatgggtaa ggcaacgttt catcatctag cgcgcgggca
     3541 cagcgcggca ggtggccagc aggtgagcac aatgtcggtt ccaactctaa taagcttggt
     3601 acgtgctcaa ggaccagcgc cacatttgcg tgatgcactt ccgggtctcg aaaagtacag
     3661 gcggtggcta tatgccagac ctcaatctaa tccgtagctg ctgcggacgg gccagaagcg
     3721 cagtgcttca tcaatcgctg gcggcggccc gccgcagagc acgacgttag agtgccttag
     3781 aaggtgcgca cacttccacc cgggaagaag aacgcgatcc tcgcgccgca cgtcgtgatc
     3841 ttaggcgccg gggcgagcgt cgccgcatcc aaacattggg gtgagattgg gtgcccccta
     3901 ccttcgatgc aaaatttggt cgacgttctg gagttacgag aagaggttga atcgcggggt
     3961 tactcggccg aagggatgga ctttgaatca ttctatgatg atcttgtgac atatggcacg
     4021 gatcccgaac tcaagcgtct tattgagcaa cgaacgtacg agtacttttc aagtctcggg
     4081 ctcccggata cgcccaccat ctatgactat ctagttctct cgcttagaaa caaagatctc
     4141 attgcgtctt tcaactggga cccatttctg cttcaggcgt ataggcgcaa cgggggtcga
     4201 ggaccggagt ccttgccggg cgtggcgttc ttgcatggaa acgtttgtat cggagtctgc
     4261 cacgcagata aagtatgtgg cttgaatggc ggtcgttgtt caaaatgcgg taccaatttc
     4321 gagccctcca agttgctcta cccagtcaaa cagaaggatt atgcgtccga cccttttatt
     4381 gcgagtgagt gggatcgact tcgcgatcat ttgtctcgcg cttactttct aacgatcttc
     4441 ggatatagcg ctccgaagac cgacgtcgag gcgcgacgcc taatgcttga agtgtggcaa
     4501 gagaatcatc atcttgaact cgcggaagtt gagatcattg agccgctggg gtcaggtcct
     4561 tacttcatag tgcacgacat tgatggagca atgagttgaa ggcctaaccc ttctcgcacc
     4621 gcatgctttc cccggtgcag ccgttagcgt gccccgaata ttggtgctag gctattgcac
     4681 atgagcgatg ccgacgttgc agagattctc aagctaccgc ccgaggagcg gctccgtctg
     4741 gtcgaactga tctgggagag ccttgctgcg accccctctg cggttccact gggcgacgct
     4801 caccgtgcgg cgatagacga ggaactcgcc gagcatcgac gcaacccgaa tgacgtcctg
     4861 acgcacgaag aactgatctc cggggttcgc aagccgcgat gacactgccg gtggtttatc
     4921 gccggcgtgt ccaacttgat ctggctgccg cgttcgactg gtacgaagaa cagcgaaccc
     4981 ggctcggcga ggaattcctc tccgcggtgc agtcaacctt ccagaccatc gagctgtatc
     5041 ccgagatttt cgagtccatt catggcgagg ttcgccgagc cattgttcct cggtttccct
     5101 tcgcagtctt ctacatcgtg gagccgcggc gcactgtcat tctcagagta ctgcatacgg
     5161 cacgtaatcc agcgctttgg cccgttccca agcgtcaggc acgctaatcg ctgcatcgac
     5221 accgaaccct tcgaaaaaaa cgagggggtc gttttgattc ttgtttctct cggcgttctc
     5281 ggcggtttaa gaagccgtga gatcacaaga tcgggaaatc gtgagagggg tttaccttcc
     5341 catctcccga tctgacgctc tcccgccctc gcgttatttt gttcttcttg gcggttgatt
     5401 tgaggatagc gggtacaatt ccccgcacca tgatcgccgt cacgctgcgc tggatcgacg
     5461 tcaacatcct ctccctcggg cgggagctgc ggctctccta tctcccgccg ctgatggttt
     5521 acgtcgccta cggcatctcg ggcctgaccg gcatcgtcgg cactttcttt gtcaaggact
     5581 acctcggact ctccgccgcc tttctcgccg cgctcggctt ctgggccggc atcccatggg
     5641 tgctcaagat gccgctcggg cacatcgtgg atctcatctg gcgctggaaa agctggctcg
     5701 tgttcctcgg cgccgggctg atcgcggcga gcctcgccat tatggtgggt ctgctcaccg
     5761 accgcaccgc gatgaccgcg ttcatgcctg ccgaagcctg gttcgtgctg tcggtgttgc
     5821 tttcaccggt cggctatgtc gtccaagacg tggtagccga cgcgatgacg gtggaggccg
     5881 tgccgcgcgt ggacgagcgc ggcacgcccg ttccgcagga ggcccgcaag ctcatgaaca
     5941 ccaccatgca gacgctgggc cgggtggcgc tgatcggcgg cctcgccctg gtggcggcga
     6001 tcaatctcta cctgttcgcc gacgtcgaga agctgtcgga ggcgcaaaag gtggcgatct
     6061 accggcagat ctatctcacg gcgatggtaa tccccgtcat ttccgttctg ggcgtcgtat
     6121 tcgcgttttt cctcaggcgc agggcgatcc agcgtctcgt tgcgcagggt atgga
//
LOCUS       DBNGWZ010000237         3866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1410064, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000237 DBNGWZ010000000
VERSION     DBNGWZ010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1410064"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="AC16CX_07135"
     CDS             complement(<1..472)
                     /locus_tag="AC16CX_07135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3012324.1"
                     /translation="MFVEKPSIHRYSVLDAGKLTSLFRSGEIFFAAGDDFSDITKLAA
                     FVENSLRSNNVYILGKDPRYEAFIFIPMHTTSCYSAHFAVREGHRGREAARHLVDSAQ
                     WVFRNTHSRAIMGFILDGNRRARMLASFVGMRRIGKSDGTIKFKGDMVGEVIYQC"
     gene            complement(474..893)
                     /locus_tag="AC16CX_07140"
     CDS             complement(474..893)
                     /locus_tag="AC16CX_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012325.1"
                     /translation="MKRATAAALLALLAGCLAPGNLPPREYYVLDDLAKAAAPKRATA
                     IDRVLLVNHNAATQRLQLENEWKGYLAAEQFDSSETQQLMQSAGSMGTELTGSIERLL
                     RDTNITNKEEAIAALMSQYRAQLNTVAATAGITLEWS"
     gene            complement(890..1837)
                     /locus_tag="AC16CX_07145"
     CDS             complement(890..1837)
                     /locus_tag="AC16CX_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3012326.1"
                     /translation="MEPEARYTVVGAGLLALVLVMLLTMVWLTRTGARADFRYYTIYF
                     ERQSLEGLQVGGDVNMRGVKVGRIENYSISRDNINRVKVTIRIERKSPVSDNTEAIVS
                     RNILTGLARINLVTPGKPGPEITYIQPSEQYPVIPEGQSDMDQLTDAVNRLALTGASA
                     LDSMDQLLNKENREAFSATLANLQRLSGALAQRVGRLDEVANAFSKNAAELGKASRDI
                     AAAMERMSAAAQPAASQAEATLRDLSRAVQALERETTALAKRLDQATDSGALEIRATA
                     QELRASAEILSRAADRLQDPRALIFGPSPAQLGPGERLR"
     gene            complement(1827..2606)
                     /locus_tag="AC16CX_07150"
     CDS             complement(1827..2606)
                     /locus_tag="AC16CX_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012327.1"
                     /translation="MNAAAAVLEVDSVVTRFGEQLVHDGVSFSVARGQIVGMIGESGS
                     GKSVLVREIIGLLRPTAGEVRLLGTNVWRAPAEELAALRRRFGMTFQDGALFSSLNVA
                     QNVATPLIEHAAVPPHLLGPLVGLRIALAGLPLEAATKMPSELSGGMRKRAAVARALA
                     LEPEVLFLDEPTSGLDPITARAFDRLLRFLCDDLGLTILLVTHDPDTLLGIVDRVIVL
                     ARGKVIAEGPVKDVMQVDDPWLQSYFKMRKMFEGAADNHGT"
     gene            complement(2603..3670)
                     /locus_tag="AC16CX_07155"
     CDS             complement(2603..3670)
                     /locus_tag="AC16CX_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR3012328.1"
                     /translation="MLFLAGTWRLPRLAEIDDALAAAGIPAAPLTLDGSALAVLDTAA
                     ALTLLRRLASAGAGIERLVNFKAGHARVVEAVRARLDDIAVELPRRRRGALAALGVQA
                     LDLRDLLAGHLDFLGRSAAELGGAALRPRTLRLKELAAQFEQVCLNAIPVVMLVTFLI
                     GVVFAYLLGIQAEKFGANIFVVDGIAIGTSRELSPIIVAVIMAGRSGAAFTAQLGTMR
                     LTEEIDAIRTLGLSPMQVLVLPRLIALMVALPLLVFIGDLSSIAGAVFVADAMLDITP
                     VTFIERLHSELPLKHLVVGLVKGPVFAAFIALIACRMGLTVSRDTRSIGINTTSTVVQ
                     SIVAVILLDAFFAVYFQEMDI"
BASE COUNT          714 a         1241 c         1236 g          675 t
ORIGIN      
        1 tacactggta aataacttcg ccaaccatat cgcctttaaa tttaatagtg ccatcggact
       61 tgccgattct cctcatgccg acaaatgacg ccaacattct ggcccgacgg tttccatcca
      121 aaatgaaacc catgatagcc cgactatggg tattgcgaaa cacccattgc gcagagtcca
      181 cgagatgcct ggccgcctcc ctcccacgat gtccctccct tacagcaaaa tgagcagagt
      241 agcacgaggt ggtgtgcatt ggaatgaaaa taaacgcttc gtatcgaggg tcttttccca
      301 aaatataaac attattcgat ctcaggctgt tttctacgaa tgcggcgagt ttggtaatat
      361 cggaaaaatc atcgccagct gcaaagaaaa tttcaccaga tcgaaaaaga gaagtcaatt
      421 ttcctgcatc gagaactgag taacgatgaa ttgatggttt ttccacaaac atatcaactc
      481 cattcaaggg tgatcccggc ggtggccgca acagtgttta attgtgcgcg atactgagac
      541 atcaatgcgg cgatagcctc ttctttgttt gttatattgg tatcccgcaa taatctctcg
      601 atgcttccag tcagttcggt ccccatagac ccagctgatt gcattaattg ctgtgtctct
      661 gagctgtcga attgctctgc cgctaaatac cccttccatt cgttttcgag ctgcagcctt
      721 tgggttgcgg cattatggtt gacgagcagc acgcggtcga ttgccgtggc gcgcttcggc
      781 gcagccgcct tcgccaggtc gtcgagaacg tagtattcgc gcggcggcag attgccgggc
      841 gcgaggcaac ccgcgagcag cgcaagcagg gcggcggcgg tggcgcgctt catcggagcc
      901 tctccccggg accgagttgc gccgggctcg ggccgaagat cagagcgcgc gggtcctgca
      961 ggcggtcagc ggcgcgggaa aggatctcgg cgctcgcgcg cagttcctgc gccgtggcgc
     1021 ggatttcgag cgcgccggag tcggtggcct ggtcgagccg cttcgcgagc gccgtcgtct
     1081 cgcgctcgag cgcctgcacc gcccgcgaca ggtcgcgcag cgtggcctcg gcctggctcg
     1141 cggcaggctg cgcggccgcg ctcatgcgct ccatggcggc ggcgatgtca cggcttgcct
     1201 tgccgagctc ggccgcgttt ttcgagaacg cgttcgccac ctcgtcgagc ctgcccacgc
     1261 gctgggccag cgcgcccgaa agccgctgca ggttggcgag cgtggcgctg aacgcctcgc
     1321 ggttttcctt gttgagcagc tggtccatgc tgtcgagcgc actggcgccg gtgagcgcga
     1381 gccggttgac cgcgtcggtg agctggtcca tgtccgactg tccctccggg atgaccggat
     1441 actgctcgct gggctggatg tacgtgatct cgggtccggg cttgcccggc gtgaccagat
     1501 tgatccgcgc cagaccagta aggatgttgc gcgagacgat cgcctcggtg ttgtcgctga
     1561 ctggcgactt gcgctcgatg cggatggtga ccttgacgcg gttgatattg tcgcgcgaga
     1621 tcgagtagtt ctcgatgcgc cccaccttga ccccgcgcat gttgacgtcg ccgccgacct
     1681 gcaggccttc gagcgactgg cgctcgaagt agatcgtgta gtagcggaag tccgcgcgcg
     1741 cgccggtgcg cgtgagccac accatcgtca acagcatgac gagcaccagc gcgagcagcc
     1801 cagcgcccac caccgtgtag cgcgcctcag gttccatggt tgtccgccgc tccctcgaac
     1861 atcttgcgca tcttgaaata agactgcaac cacggatcgt cgacctgcat gacgtccttt
     1921 accgggcctt cagcgatcac cttgccacgg gcgagcacga tcacgcggtc gacgatgccg
     1981 agcagggtgt cagggtcgtg cgtgacgagc aggatcgtca acccgaggtc gtcgcacagg
     2041 aaccgcagca gtcggtcgaa ggcgcgtgcg gtgatcggat cgagtcccga cgtcggctca
     2101 tcgaggaaca gcacctccgg ttcgagcgca agcgcgcgcg ccaccgccgc gcgcttgcgc
     2161 atgccgccgg aaagctcgct cggcatcttg gtcgcggcct cgagcggaag gccggcgagc
     2221 gcgatgcgca gccccaccag ggggcccagc agatgcggcg gcacggcggc gtgctcgatc
     2281 aggggggtgg cgacgttctg cgcgacgttg agcgaggaga acaacgcccc gtcctggaac
     2341 gtcatgccga aacggcggcg cagcgccgcg agctcctcgg ccggggccct ccagacgttg
     2401 gtgcccagca gccgcacctc ccccgccgtc ggccgcagca gtccgatgat ctcccgcacc
     2461 agcaccgatt tgccgctgcc gctctcgccg atcatcccca cgatctggcc gcgcgccacg
     2521 ctgaacgata cgccgtcgtg caccaactgc tcgccgaagc gggtgacgac cgaatccacc
     2581 tcgagcacgg ctgccgcggc gttcaaatgt ccatctcctg gaaatagacc gcgaagaacg
     2641 catcgagcag gatcaccgca acgatgcttt gcaccacggt ggaggtggtg ttgatgccga
     2701 tggagcgcgt atcgcggctg acggtcaagc ccatgcgaca ggcgatgagc gcgatgaatg
     2761 cggcgaacac cggccctttc accagcccca cgacgagatg cttgagcggc agctccgagt
     2821 gcaggcgctc gatgaaggtg accggcgtga tgtcgagcat cgcgtcggcc acgaacaccg
     2881 ccccggcgat gctggacaag tcgccgatga acaccagcag cggcagcgcg accatcaacg
     2941 cgatgaggcg cggcagcacc agcacctgca tcggcgacag gccgagcgtg cggatcgcat
     3001 cgatctcctc cgtgagtcgc atcgtgccga gctgcgcggt gaacgcggcg cccgaccggc
     3061 ccgccatgat caccgcgacg atgatcggcg agagctcacg gctggtgccg atggcgatgc
     3121 cgtccacgac gaatatgttg gcgccgaatt tctcggcctg gatcccgagc aggtaggcga
     3181 acaccacgcc aatcaggaac gtgacgagca tgacgaccgg gatcgcgttg aggcacacct
     3241 gctcgaactg ggcggcgagt tccttcagcc gcagcgtgcg cgggcgcagc gccgcgccgc
     3301 ccagctcggc tgcggaccga ccgagaaagt cgagatgccc cgcgagcaag tcgcgcaaat
     3361 cgagcgcctg cacgccgagc gcagccaggg cgccgcggcg gcgtcgggga agctccaccg
     3421 cgatatcgtc cagccgcgcg cgcacggcct cgaccacgcg cgcgtggccc gccttgaagt
     3481 tgacgagccg ctcgatgccc gcgccggcgg aggcgagccg gcgcagcagc gtcagcgccg
     3541 ccgcagtgtc gagcacggcg agagccgagc cgtcgagcgt caggggcgca gccggtatgc
     3601 ctgcggcggc gagcgcgtca tcgatctcgg cgagccgggg aagacgccac gtgccggcga
     3661 gaaaaagcac ggcttttcct ccgtcccgtt cgagccggca atagcgttcc ggggtgttca
     3721 tctcgaagag agaatatggg ctgggcggcg tacgggcgca aacctgatct tcgactatat
     3781 caaatggaat cgaacccggg gtggccggcg atctaccggc atttcagcag acgttcacgc
     3841 ccggcctcgt gcgtgacgtt ctggac
//
LOCUS       DBNGWZ010000238         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_778822, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000238 DBNGWZ010000000
VERSION     DBNGWZ010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_778822"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="AC16CX_07160"
     CDS             complement(<1..423)
                     /locus_tag="AC16CX_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3012329.1"
                     /translation="MKIQFDPSTPEAEAVRQRYLKVDAATVSDVLDQLGYLHQGLAGE
                     FAPFPETAGKLAGWAYTICGEMRPYADGGDPDKMKAVDGIKPGCVSVWCGQGDGVCFF
                     GELIALGMKSRGCAGALVDGGIRDIEWIAKQQFPVYARY"
     gene            complement(548..1363)
                     /locus_tag="AC16CX_07165"
     CDS             complement(548..1363)
                     /locus_tag="AC16CX_07165"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF009829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR3012330.1"
                     /translation="MRVGIIGGGTIARLFLEHIRRGDLGRTRVVAIAGRSDRSRGKLL
                     AKAFRVPFVSGVTQLLAKRPDVVVEAASHDAVREYGESLLKKGVGLIVLSCGALCDDA
                     LRRRLERAAAKSGALLSVPSGGIGGLDALKAACVAGVDEVTITVTKPSAAWKGIAYVE
                     KLDVDLDHLREPRVLFEGPAREGVPHFPANVNIAAVLSMAGIGFDKTRLKVVADPALR
                     YNTHYIEVRGRTGNFSIKLENVPAPENPKTAWLACYSALAALKAAKSPVRYGT"
     gene            complement(1501..>2055)
                     /locus_tag="AC16CX_07170"
     CDS             complement(1501..>2055)
                     /locus_tag="AC16CX_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3012331.1"
                     /translation="ANAIFLVARRELRDPNFHEAVVLVTHPRQGGPWGVIINRPLEHR
                     LSELFPEHSTLKNSKEMVYFGGPVARQGLVFLVRSLKPPPQSIQLLRDVYFASDADWI
                     DELLSRPDPTRGLRVFAGYSGWAPGQLQSEIARGSWHVLPADAETIFNMDPGRIWPEL
                     IQRAATRHTRFFGAPALESVSGEW"
BASE COUNT          365 a          710 c          626 g          354 t
ORIGIN      
        1 gtaacgggcg tacaccggaa actgctgttt cgcgatccac tcgatgtcac ggatgcctcc
       61 gtccaccaga gcgcccgcgc agccgcgcga cttcatgccg agtgcgatca attcgccgaa
      121 gaagcacacg ccatcgccct gcccgcacca cacgctcacg cagccgggct tgatgccgtc
      181 caccgctttc atcttgtccg ggtcgccgcc atcggcgtac ggccgcatct cgccgcagat
      241 tgtgtaggcc cagccggcca gcttgcccgc ggtctccgga aacggcgcaa actcccccgc
      301 cagcccctgg tgcagatagc cgagctgatc gaggacgtcc gacacggttg cagcgtccac
      361 cttcaaataa cgctggcgga ccgcttcggc ttcgggggtg gatgggtcga actgaatctt
      421 cataatgaga aaagcgtggg ttgtggattg tgggtcgatc tactcgttcc aactctctct
      481 tccgatcggg ggacaggttg tccggctcaa aatcgatcca cgacccacga tccacgatcc
      541 acggggttca ggtgccatag cgcaccggcg acttcgccgc cttcagcgcc gccagcgcgc
      601 tgtagcaggc gagccaggcg gtcttgggat tctccggcgc cggcacgttc tcgagcttga
      661 tgctgaagtt cccggtgcgg cccctgacct ctatgtaatg ggtgttgtac ctgagcgccg
      721 ggtccgcgac caccttgagc cgggtcttgt cgaagccgat ccccgccatg gagagcacgg
      781 ccgcgatgtt cacgttggcg ggaaaatgcg gcacgccctc ccgcgccgga ccctcgaaca
      841 gcacgcgcgg ctcgcgcaag tggtcgagat ccacgtcgag cttctcgaca taagcgatac
      901 ccttccacgc agcggacggc ttggtgaccg tgatggtgac ttcatccacg cccgccacgc
      961 aggccgcctt cagcgcatcc agcccgccga tgccgccgga gggcaccgag agcaacgcac
     1021 cgctcttcgc cgcggcgcgc tcgagccgcc tgcgcagcgc gtcgtcgcac agcgccccgc
     1081 acgacagcac gatgagcccg acgcccttct tgagcaggga ttcgccgtac tcgcgcaccg
     1141 catcatggga agccgcttcc acgacgacgt cgggtctttt cgcaagcaac tgtgtaaccc
     1201 cagacacaaa cggcacacgg aaagcttttg ccagcagctt gccgcgcgaa cggtcgctgc
     1261 ggccggcgat cgcgaccacc cgcgtgcgac ccaggtcgcc gcgccggatg tgctcgagaa
     1321 acagccgcgc gatggtgccg ccgccgatga tccctacccg cattgatcga gtctcccggg
     1381 ttctattcta atcccgggac tcgaaccgcg tgactcgcgg ctcgtgactg gtgactggtg
     1441 gttttttgat cctgccttcc caccatcggg tgagaaaaaa acaacaggtc aactcacggc
     1501 tcaccactca ccactcacgc tttcaagcgc aggtgcgccg aagaagcggg tatgtcgtgt
     1561 ggcggcgcgc tggatcaact ccggccagat gcgccccggg tccatgttga atatcgtctc
     1621 ggcgtccgcc ggcagcacgt gccagctgcc gcgtgcgatc tcgctttgca gctgcccggg
     1681 cgcccatccc gagtagccgg cgaacacgcg caagccgcgc gtcgggtcgg gacgactcag
     1741 gagctcgtct atccagtcgg cgtcggatgc gaaatagacg tcacgcagta gctgaatcga
     1801 ctgcggcggc ggcttgagcg aacgcaccag gaacaccagc ccctggcgcg ccaccggccc
     1861 gccgaaatag accatctcct tgctgttctt cagcgtcgag tgctcgggaa acaactcgct
     1921 caggcgatgc tcgagcggtc ggttgatgat gacaccccag ggcccgccct ggcgcgggtg
     1981 cgtcaccagc acgacggcct cgtggaagtt ggggtcgcgc agctcgcgcc tggcgacgag
     2041 gaagatcgca ttggc
//
LOCUS       DBNGWZ010000239         7729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_401912, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000239 DBNGWZ010000000
VERSION     DBNGWZ010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_401912"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="AC16CX_07175"
     CDS             complement(<1..974)
                     /locus_tag="AC16CX_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012332.1"
                     /translation="MAGRFTFAQAAVALLIALFLLVFLAIPVFTVIYVAFTNADGSLT
                     LSHFQSFFHLTLMRESFYNSLIVGGMSVVISSVFALPLAYFITRFQFRGALLIQTLGV
                     LPLIMPPFVGATAMQLLFGRSGSLNLILGDWFGINIPIMEGLNAVIWVEALHYFPFIL
                     LNLTVALNNIDRAMEESAQNLGASGFRLFRRIVFPLAMPGYMAGAALVFLKVFDDVAT
                     PLVLNVTNILAAQAYLRITSIGIDDPIGYVASVIMVAASMLALWGSTWAMRGKEYATL
                     QRGGSTLATKKLKPWQAAIAYGWIVFVLLLVLSPHVGILLLSFSRVWS"
     gene            complement(1209..2549)
                     /locus_tag="AC16CX_07180"
     CDS             complement(1209..2549)
                     /locus_tag="AC16CX_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012333.1"
                     /translation="MNPCQRFSSALLGIIGLLAAAMPGAAVAQQQVVVVTSFPKELTE
                     AYKKAFESRNPGIRVEILNKPTPVGVAYVREAPAANKPDVFWVSAPDAFEVLSAEKLL
                     AKIESPAKEVPAKIGNYPINDPKGFYLGQALAGYGIMWNTRYLAANKLPEPREWADLV
                     KPVYFGHVAMSSPSRSGTTHLTVETILQGEGWEKGWRQMLMIAANSAAITERSFGVPD
                     GVNNGQYGIGLVIDFFGLAAMASGFPTKFVYPSVTAIVPANIGMVAGAKNAENARKFI
                     NYTLSPEGQELLFDPKISRLPVLPSVYKKAPAGFPNPFTGKIKPKVNFDSELSESRYY
                     VVSSIFDHTITFRHKELTAAARAIYQAEARLGARPNAQAQKLLAEARELAFSPVVSEE
                     KSKDKAFIALFRETKRSAEKTKQITGLEEYWNSTARKNYVRAAELANQAAAMAK"
     gene            complement(2793..3149)
                     /locus_tag="AC16CX_07185"
     CDS             complement(2793..3149)
                     /locus_tag="AC16CX_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012334.1"
                     /translation="MEPIKDSSRFYEVERRQYHAERPGFRINELQISPTQQVPWHYHS
                     NIQDTFYVIEGQLRLFLREPKEEVRLGPGDTYSVPPRRPHLVTNGGDVSATFLVLQGI
                     GEYDFVPLVPKPSRPK"
     gene            complement(3172..4158)
                     /locus_tag="AC16CX_07190"
     CDS             complement(3172..4158)
                     /locus_tag="AC16CX_07190"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR3012335.1"
                     /translation="MKHRACLTLCLLPFAALAGGSNYGITPGAHPNFAGKVSEWPVPT
                     PRFARDPAPAPDGSIYIAVMSGNKVARFDPKAKSFKEWDLPDGHRPHGLLVDKQGIVW
                     TTGNGNGTIGRLDPRTGEVKEFKTPSGGGGPHTLVITDDGATIWFTLQSGNKVASLDT
                     KTGTIKEYPTSGGPYGIALDKVGNVWFCRMGDNKMGRLDPKTGKMSEVDMGRGSRPRR
                     VATAPDGMLWVTLYGNGRLAKLDPVAMKVVKTYPLPGGDAGPYAVTVDGGGMVWANEI
                     NTDTVVRLDPKTEQMRVVQLPSNNVGIRKMVTDASGRLWYMGSHNGRLGVVE"
     gene            complement(4285..5094)
                     /locus_tag="AC16CX_07195"
     CDS             complement(4285..5094)
                     /locus_tag="AC16CX_07195"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR3012336.1"
                     /translation="MTAIHLSPASAETDVLLREDNSGIATLTLNRPDQYNALSAALLA
                     ELQSALSAIAEDDSVRIVVISGAGKAFCAGHDLKEIQAHPDAGFSRDVFEHCRRLMNA
                     LTRLPQPVIARVHGPAYAAGCQLVAQCDLAVASTEARFATSGVNFGVFCSTPGVPLAR
                     NVSRKRALEMLLTGEPIDAASALAYGLVNRVVPPARLDEEVQNLARSILGKSPAVIAA
                     GKKAFYEQVDESLAKAYRVATAAMVDNLQTEDGKEGIAAFIEKRTPRWKNK"
     gene            5193..6941
                     /locus_tag="AC16CX_07200"
     CDS             5193..6941
                     /locus_tag="AC16CX_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3012337.1"
                     /translation="MARHALFAVIVVCAILLGATGATERYDLKLLDLQFRLLRAWSPQ
                     AVARDVVIVGVDEDTVKRFPEPITLWHAHLGRFLGAMALARPAAVGMDIVLPDRSFEA
                     VMPGSDRALLKGLLEARRGFPLVLGLTVDPSGKPRPIHPPFLSVAGPGASGYALFPVD
                     RDGVVRRFDERLAQGGDAVPTLSGQLARRLNVEPRAGLVDYWRGAPYNYVPLQQVLQW
                     AEAGDEPALARAFRDKPVLVGMVFQFEDRLSAPVQLAAWEPEAPNTAGVLLHAQVLRN
                     LLNGGFVQATPAAATVATCVVLAALWFVSGSTSLIALLLAAAVALIAGASTWLLAQGW
                     FFPALAPALTAALALGGRNGYDTLLKLRERRRLRASFGGYVSPSVMEEILAGRIQPEL
                     GGVNQFVCVLFSDIRGYTTRSERMTPEQVIRFLNRYFEQTVALIHERGGAVMSFMGDG
                     IMAVFGAPKPLDNPCREAFEAARAMLKYVAELNGRFRAEGEAPMDVGIGLHAGEAVIG
                     HVGSSSRHDYTVIGDVANVASRLESLTKEAGYRVVVSKVVAEQLSADGALASLGPMAI
                     KGHSPVEVYGYDKISV"
     gene            7182..>7729
                     /locus_tag="AC16CX_07205"
     CDS             7182..>7729
                     /locus_tag="AC16CX_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012338.1"
                     /translation="MKLWLRSCSWLLAAIAASWLTGIATPALAQSVAMVTDVTGKAGV
                     QGASGRGALTLMSEIAMDARVQLDGGARLVAVYLSSGDEYAFTGPAQIHFRASEPQVL
                     SGAAPQKRSSPLAKSGKDATIRVSGVAQAGFVMRGGRASGRIRLLSLTGTRTLEYSPE
                     FRWQQTNNLTRYQFRLLDDMGKP"
BASE COUNT         1259 a         2398 c         2608 g         1464 t
ORIGIN      
        1 aagctccaga cccggctgaa cgagagcagc aggatgccga cgtgggggga gagcaccagc
       61 aacagcacga aaacgatcca gccgtaggcg atcgccgcct gccacggctt gagcttcttg
      121 gtggcgagcg tgctgccccc gcgctgcagc gtggcgtatt ccttcccgcg catcgcccag
      181 gtcgagcccc acagcgccag catcgaagcg gcgaccatga tcacgctcgc gacatagccg
      241 atggggtcgt cgatgccgat cgaggtgatg cgcagatacg cctgcgccgc gaggatattg
      301 gtgacgttga gcacgagcgg cgtcgcgaca tcgtcgaaca ccttcaggaa caccagcgcc
      361 gcgcccgcca tgtagccggg catcgccagc gggaagacga tgcggcggaa caggcggaag
      421 ccgctcgcgc cgaggttctg cgcggattcc tccatggcgc ggtcgatgtt gttgagcgcg
      481 accgtgaggt tgagcaggat gaacggaaag tagtgcagcg cctcgaccca gatgacggca
      541 ttgaggccct ccatgatcgg gatattgatg ccgaaccagt cgcccagtat gagattgagc
      601 gagccgctgc gtccgaacag cagttgcatc gcggtcgcgc ccacgaacgg cggcatgatg
      661 agcggcagca cgccgagcgt ctggatcagg agcgcgccgc ggaactgaaa gcgcgtgatg
      721 aagtaggcga gcggcagcgc gaagacggat gagatcacga cgctcatgcc gccgacgatc
      781 aggctgttgt agaacgactc gcgcatgagc gtgaggtgga agaacgactg gaagtgcgac
      841 agcgtcaggc tgccgtcggc gttggtgaac gcgacgtaga tgacggtgaa gaccgggatg
      901 gcgaggaaga cgagcaggaa cagcgcgatg aggagggcga ccgccgcctg ggcaaaagtg
      961 aagcgcccgg ccatcgcccg catgagagcg ggtcggcgca gggcggcgct cgtgtcggtc
     1021 ggcggcatga cggaggacgc cgggattcta ccttgcctga gcccttacgc gcgcaccgcc
     1081 gggcctgcaa tcaggttgaa tcagaccgcc cgtcgatgaa cgcgtgccgc cgcttagcga
     1141 tcagctttcg gcggtcagct taaatcgcat ctgtttcaag ctgacagccg cgagctgacg
     1201 gctgaacgct acttcgccat cgcggcggcc tggttggcca gctcggccgc ccgcacgtag
     1261 ttcttgcgcg ctgtcgagtt ccagtattcc tcgaggccgg tgatctgctt cgtcttctcc
     1321 gcgctgcgct tggtttcacg gaacagcgca atgaacgcct tgtctttcga cttttcctcg
     1381 ctgacgacgg gcgagaaggc gagctcgcgc gcctccgcca gcagcttctg cgcctgcgcg
     1441 ttgggcctgg ccccgagccg cgcctccgcc tgatagatgg cgcgcgcggc ggcggtgagc
     1501 tccttgtggc ggaacgtgat ggtgtggtcg aagatcgagc tcaccacgta gtagcgcgat
     1561 tccgagagtt ccgagtcgaa gttcaccttg ggcttgatct tgccggtaaa ggggttgggg
     1621 aaccccgccg gggccttctt gtagacgctc ggcagcaccg gcaggcggct gatcttcggg
     1681 tcgaacagca gctcctggcc ttccggggaa agcgtgtagt tgatgaattt gcgcgcgttc
     1741 tccgcgttct tcgcgcccgc caccatgccg atgttggccg gcacgatcgc ggtcaccgaa
     1801 ggatagacga acttggtcgg gaagccggac gccatggcgg cgagcccgaa gaaatcgatg
     1861 acgaggccga tgccgtactg cccgttgttg acgccgtcgg gaacgccgaa gctcctttcg
     1921 gtgatcgcgg cgctgttggc ggcgatcatc agcatctgcc gccagccctt ctcccagccc
     1981 tcgccttgca ggatggtttc cacggtcagg tgcgtggtgc cggagcgcga cggcgaggac
     2041 atcgcgacgt ggccgaaata aaccggcttc accagatccg cccactccct gggttccggc
     2101 agtttgttgg cggcgagata gcgcgtgttc cacatgatcc cgtagccggc gagcgcctgc
     2161 ccgagataga accccttggg atcgttgatc ggatagttgc cgatcttggc cggcacttcc
     2221 ttggccgggc tctcgatctt ggcgagcagt ttctccgcgg aaagcacttc gaacgcatcc
     2281 ggcgcggaga cccagaacac gtcgggtttg ttggctgcgg gcgcttcgcg gacgtacgcg
     2341 acgccgaccg gcgtcggctt gttgaggatc tcgacccgga taccgggatt gcggctctcg
     2401 aacgccttct tgtaggcctc ggtgagttcc ttggggaacg aggtaacgac gacaacctgc
     2461 tgttgcgcca ccgccgctcc cggcatggct gcggcaagca atccgatgat gcccagcagg
     2521 gccgaactga aacgttgaca gggattcatt gctggtctcc tcgtggatgg tatgtcattg
     2581 ataattattg actgccgatg cccggacaat gttgatttaa atcaatcccc ggattccagc
     2641 ccgggccgag acgcggtaaa gcgtgagtcg cgagtcgtga ttcgacctgt gactcttcgc
     2701 gaaggattgt tcctgtcccc tttgtgggtc gaaatgggcg cggcgaacca gtttatcgtg
     2761 ttgatcggcg tctccgcggc cgggagaacg cttcacttcg gtcgcgaggg cttcggcacg
     2821 agcgggacga agtcatactc gccgatgccc tggagcacga ggaaggtcgc cgagacatcc
     2881 ccgccattgg tgactaggtg cgggcggcgc ggcgggaccg agtaggtatc accgggcccg
     2941 agccggacct cctctttggg ctcgcgcagg aagagcctga gctgtccctc gattacgtaa
     3001 aacgtgtcct ggatattgct atggtagtgc catggcacct gttgggtcgg cgagatctgg
     3061 agctcgttga ttcggaagcc gggccgctcg gcatgatatt gccggcgctc gacttcgtag
     3121 aaacggctcg agtccttgat cggttccatt gtttcctccg tcgcccggct tctactccac
     3181 gactcccagt cgtccattgt gcgagcccat gtaccacaac cgcccgctcg catcggtcac
     3241 catcttgcgg atgccgacgt tattcgaggg gagctgcacg acgcgcattt gctcggtctt
     3301 tggatcgagg cgtaccaccg tgtcggtgtt gatctcgttg gcccagacca taccgccgcc
     3361 gtcgaccgtg accgcgtacg gcccggcatc gccgcccggc agcggatacg tcttcacgac
     3421 cttcatggcg acggggtcca gcttggcgag cctgccgttg ccgtagagcg tgacccacag
     3481 catgccgtcg ggcgccgtag cgacgcggcg cggccgcgag ccgcggccca tgtccacctc
     3541 gctcattttt ccggttttcg gatcgagccg gcccatcttg ttgtcgccca tgcggcagaa
     3601 ccagacgttg cctaccttgt ccagcgcgat tccgtaaggg ccgcccgagg tggggtactc
     3661 ttttattgtt ccggttttgg tgtcgaggct ggcgaccttg ttgccgctct gcagggtgaa
     3721 ccagatggtg gcgccgtcgt ccgtgatcac cagcgtatgc ggcccgccgc cgccggacgg
     3781 agtcttgaac tccttgactt ccccggtgcg gggatcgagc cggccgatgg tgccgttacc
     3841 gttgccggtg gtccatacga tgccttgctt gtccaccagc aggccgtgtg gacgatggcc
     3901 gtcgggcaaa tcccattcct tgaagctttt cgctttcgga tcgaagcgcg cgactttgtt
     3961 gccgctcatc actgcgatgt agatgctgcc gtcgggcgcg ggggcgggat cgcgcgcgaa
     4021 gcgcggcgtc ggcaccggcc actcgctcac cttgcccgcg aaattcgggt gcgcaccggg
     4081 cgtgatgccg tagttggagc caccggccag cgccgcgaag gggagaagac agagggtcag
     4141 acaggcgcga tgcttcattc tcaggctcca actatagtga ggaatgtggg atggagtgtc
     4201 gaggaatgag tgtcgggtga aaagaatgtg aatgggcgcg catccctcaa cacgtgacac
     4261 tcgtcactcg acacccgaca ctcgtcactt gttcttccac cgcggcgtgc gcttctcgat
     4321 gaaggcggcg atgccttcct tcccgtcttc ggtctgcagg ttgtccacca tggccgccgt
     4381 ggcgacgcga tacgcctttg ccaggctttc gtccacctgt tcgtaaaagg ccttcttgcc
     4441 ggcggcgatg acggcggggg acttgcccag gatggagcgg gcgaggtttt ggacttcctc
     4501 gtcgagccgt gccggcggca ccacgcggtt gaccaggccg taggcgagag cgctcgcggc
     4561 atcgatcggc tctccggtga gcagcatttc cagcgcgcgc ttgcgggaga cgttgcgcgc
     4621 gagcggcacg cccggcgtgg agcagaacac gccgaaattc acgccggagg tcgcgaagcg
     4681 cgcctccgtg ctcgccaccg cgaggtcgca ctgcgcgacg agctggcagc cggcggcgta
     4741 tgccggcccg tgcacgcgcg cgatgaccgg ctggggcagc cgggtgaggg cgttcatcaa
     4801 ccgtcggcag tgttcgaaga catccctgga gaagcccgca tccgggtgcg cctggatttc
     4861 cttcagatca tgccccgcgc agaacgcttt gccggcgcct gagatcacca cgatgcggac
     4921 cgagtcgtct tctgcgatcg cactgagcgc ggattgcagt tcggcgagca gcgcggccga
     4981 cagcgcgttg tactgatcgg gccggttgag cgtgagcgtc gcgatcccgc tgttgtcctc
     5041 gcgcagcagc acgtcggttt cggcgcttgc cggggaaaga tggatggctg tcatcgagtc
     5101 aaggcgacgg cttgatccgt ccgccttgac tattataagt ccgccgtttg ccaggtacgg
     5161 ggtccgccag tcgctctgat agactttccg gcatggcccg gcacgccctt tttgcagtca
     5221 tcgtcgtctg cgcaatattg ctcggcgcga ccggcgcgac cgaacgttat gatctgaagc
     5281 tgctcgacct ccagttcagg ctgctgcgcg cgtggtcccc gcaggccgtc gcgcgggacg
     5341 tcgttatcgt cggcgttgac gaggacactg taaaacgctt ccccgagccg atcacgctct
     5401 ggcacgctca tctgggtcgg tttttgggag cgatggcgct ggccaggccg gcggcggtcg
     5461 gcatggacat cgtgctgccg gaccgcagtt tcgaggcagt gatgcccggc tcggaccggg
     5521 cccttctgaa gggcctgctc gaggcgcggc gcggttttcc tctcgtgctg ggactcacag
     5581 tggatccgtc cgggaagccg cgaccgatcc acccgccttt tctctccgtc gccggacccg
     5641 gggcgtcggg atacgcgctg tttcccgtgg accgggacgg cgtcgtgcgc cgcttcgacg
     5701 agcgcctcgc gcagggcggt gatgccgttc ccaccctgtc cggccagctg gcgcgacgcc
     5761 tgaacgtgga accgcgcgcc ggtttagtcg attactggcg cggcgcgccc tacaactatg
     5821 tgccgctgca gcaggtgctg cagtgggcgg aggccggcga cgagccggca ctcgctcgcg
     5881 ccttccgtga caagccggtg ctggtcggca tggtgttcca gttcgaggac cgcctgtccg
     5941 cgccggtgca gctcgccgcc tgggagcccg aggcgcccaa cacggcgggg gtgctgctgc
     6001 acgcccaggt gttgcgcaac ctgttgaacg gcggcttcgt gcaggcgacg cctgccgcgg
     6061 cgaccgtggc aacttgcgtc gtgctcgcag cgctctggtt cgtctcgggc agtaccagcc
     6121 tgatcgcgct gctgctggcg gcagccgtcg ccttgatcgc tggcgcatcc acctggcttc
     6181 tcgcgcaggg atggttcttt cccgctctgg cgcccgcatt gacggcggcg ctcgcgctcg
     6241 gcggcaggaa cggctacgac accttgctca agctgcgcga gcggcggcgg ctgcgcgcat
     6301 ccttcggcgg ctacgtcagc ccgtcggtga tggaggagat cctcgccggc cggatccagc
     6361 ctgagctcgg cggcgtgaac cagttcgtgt gcgtgctttt ttccgatatt cgcggctaca
     6421 ccacgcgcag cgagcgcatg acgccggagc aagtgatccg cttcctcaac cgctatttcg
     6481 agcagacggt ggcgctgatc cacgagcgcg gcggcgcggt gatgagtttc atgggcgacg
     6541 gcatcatggc cgtgttcggg gcccccaagc cgctcgataa tccatgccgc gaggcattcg
     6601 aggctgcccg tgccatgctg aaatatgtgg cggagttgaa cgggcgattc cgtgctgaag
     6661 gcgaggcgcc catggacgtc ggcattggtc tgcatgccgg cgaagccgtg atcgggcacg
     6721 tcggctcctc gtcccgccac gattacaccg tgatcggtga tgtcgccaat gtcgcctcgc
     6781 gtctcgaaag cctgaccaag gaagcggggt accgcgtcgt ggtgtccaag gtggtcgccg
     6841 agcagctgag cgcggatggg gcgctcgcct ccctcggacc gatggcgatc aagggacact
     6901 cgccggtgga ggtctatggc tacgataaga tcagcgtcta gcggtcagca ttcagcagtc
     6961 agcttgcagc aaggcgttgc atgggctgat tgcggatggc tgacagctga tcgccgcgtg
     7021 ctttcatgtg cgcggccgac ggtcgtgttt atcttgccgg ctgcttgaat atttcgcggg
     7081 atttgctaat gtttgcagca gcgtcatggg cgccacgcgc gatgtcggga atgggcgcgg
     7141 ggcagtcgga aacctaacaa aatcggacag ttgggggaga aatgaaactc tggctacgaa
     7201 gctgttcgtg gctgctcgca gccatagcgg cgtcgtggtt gaccggaatc gcgacaccgg
     7261 cgctcgcgca gagcgtcgcg atggtgaccg acgtcaccgg caaggccggt gtgcagggcg
     7321 cttccggcag gggcgcgctt accctcatgt cggagatcgc gatggacgca agggtccagc
     7381 tcgacggtgg tgcgcgcctc gtcgccgtct acctctcgtc cggcgatgaa tacgcgttta
     7441 ccggcccggc gcaaatccat ttccgcgcgt cggagccaca ggtcctgagc ggcgccgcgc
     7501 cgcagaagcg ctcgagcccg ctcgcgaaaa gtgggaagga cgccacgatc cgggtaagcg
     7561 gtgtcgccca ggccggcttc gtgatgcgcg ggggacgcgc ctcggggcgc atccggctgc
     7621 tctcgctcac cgggacgcgg acgctcgaat actcgcccga attccgctgg cagcaaacca
     7681 ataacctgac gcgctatcaa ttcaggcttc tggacgacat gggcaagcc
//
LOCUS       DBNGWZ010000240         2427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2795627, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000240 DBNGWZ010000000
VERSION     DBNGWZ010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2795627"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="AC16CX_07210"
     CDS             complement(<1..1126)
                     /locus_tag="AC16CX_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3012339.1"
                     /translation="MARKYDESSVKKLRGIEPVQRLPGMYTRTSDPTHIIQEAIDNAA
                     DEALGGHADKIEVTVHLDGSVTVTDNGRGIPVGIHPEEKISTVELVLTELHAGAKFSR
                     TDADAAYKFSGGLHGVGVSVTNALSERLEVEIKRDGAVHAIAFADGKIAKKLRKIGTC
                     GERNTGTSLRIWPNKKYFDSPKISFDDLERIVRSKAVLLPGVKVTLNVETAKETKKQT
                     WNYPDGMKGYLAELIGSAQVVAPVFDGEKYVKEGEANGFAVGEGAAWAFAFVEDDKGY
                     GESYANLIPTTDGGTHEAGLREGIFEAIKAFAEYHNLTPKGVKLLAEDAWSRTVYFLS
                     AKVLEPQFHGQTKEKLSNRDAVKLVSTMVRDPFAAWMTANE"
     gene            complement(1455..>2427)
                     /locus_tag="AC16CX_07215"
     CDS             complement(1455..>2427)
                     /locus_tag="AC16CX_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012340.1"
                     /translation="RCILLRIAIALVAATTFSAGTSFAQPYPSKPVRMIVPFAPGGGT
                     DIIARLVAQELTQTWGQTVVVDNRGGSGGIVGTQLAVQSAPDGYTMVLCSLGVSYAPA
                     LYSKLPYDTERDLLPVSLVATQPFIYVVTPSLGVSSLKELIALAKAKPGAIRYGSGGA
                     GGSSHLGTELLRTMTGMDLVHVPYKGTGPALTAMLAGEIQMQLIGISSVVPHLKSGRM
                     RALAVSGAKRSAAAPEVPTVAESGVPGYAFDVWYGMLFPARTPRAIVSKVNADINQVL
                     KSPALGQRFAAGGLEPSGTSAEEFAKMLRAEIAKWRKVVREAKIKVE"
BASE COUNT          392 a          790 c          806 g          439 t
ORIGIN      
        1 cctcgttggc ggtcatccac gccgcgaacg ggtcgcgcac cattgtcgac accagcttca
       61 ccgcgtcgcg gttggagagc ttctccttgg tctgcccgtg gaactggggc tcgagcacct
      121 tggccgacag gaagtagacc gtgcgcgacc acgcatcctc ggcgaggagc ttgacgccct
      181 tcggggtcag gttgtggtac tcggcgaagg ccttgatcgc ctcgaagatg ccttcgcgca
      241 gccccgcctc gtgggtgccg ccgtcggtgg tgggaatcag gttcgcgtac gactcgccgt
      301 aacccttgtc gtcctcgaca aaggcgaacg cccaggccgc gccctcgccg acggcgaagc
      361 cgttggcctc gccttccttc acgtatttct ccccgtcgaa gaccggcgcc accacctgcg
      421 ccgaaccgat cagctcggcg agataaccct tcatgccgtc cggatagttc caggtctgct
      481 tcttcgtctc cttcgcggtc tcgacgttga gcgtgacctt cacgcccggc agcagcacgg
      541 ccttggagcg cacgatgcgc tcgaggtcgt cgaacgagat tttcggggag tcgaagtact
      601 ttttgttcgg ccagatgcgg agcgaggtgc cggtgtttct ttcgccgcag gtaccgatct
      661 tcctgagctt cttcgcgatc ttgccgtcgg cgaacgcgat cgcgtgcacc gcgccgtcgc
      721 gcttgatctc gacctcgagg cgctcggaga gcgcgttggt gaccgacacg ccgacgccat
      781 gcagaccgcc ggagaacttg taggcggcgt ccgcgtcggt gcgcgagaac ttggcgccgg
      841 cgtggagctc ggtcagcacc agctcaacgg tggagatctt ctcttcgggg tggatgccga
      901 ccggtatgcc gcggccgttg tcggtgaccg tgaccgagcc gtcgaggtgc accgtgactt
      961 cgatcttgtc ggcgtggccg ccgagggcct cgtcggctgc gttgtcgatc gcttcctgga
     1021 tgatgtgggt gggatcggag gtccgggtat acatccccgg gagccgctgc accggctcga
     1081 tgcctcttag cttcttgact gagctctcgt cgtatttcct ggccattcgc tccgcaattg
     1141 agggaagtgg ggcagggcgt attataaccc tttgtttatc aaagtactag ccccaggagc
     1201 ccgcgcggtg gaaaccggcg acggaggcgg tgacagacac ggtgtcgcat tcgacgccac
     1261 cgtaacgcgg gctcctgaat cagaggccgc ggaggaatga ggagcgcgtc aaggggcgga
     1321 agaggggccc cgtgattttt cggggatcag acccccggtg gcgcgcaggg cggccgacac
     1381 gattgcgacg ataactcgcg atgacatatc agaggccgcg gagggaagag tgtcgaggaa
     1441 cgagtgtcga gtggctactc aaccttgatc ttcgcttcgc gcacgacctt gcgccacttg
     1501 gcgatttcgg cgcgcagcat ctttgcgaat tcctcggccg aggtgccgga aggctcgaga
     1561 ccgccggcgg cgaagcgctg cccgagcgcg ggggacttga ggacctggtt gatgtccgcg
     1621 ttgaccttgc tgacgatcgc gcgcggcgtt cgcgccggaa acagcatccc gtaccacacg
     1681 tcgaaagcgt aaccggggac gccactttcc gcgacggtcg gcacttcggg cgcagcagcc
     1741 gagcgcttgg ccccgctcac ggccagcgcc cgcatgcggc cggacttgag gtgcggtacg
     1801 accgacgaga tgccgatcag ctgcatctgg atttcgccgg ccagcatcgc cgtcaatgcg
     1861 ggcccggtgc ccttgtacgg cacgtgcacg aggtccatgc cggtcatcgt gcgcagcagc
     1921 tccgtgccga ggtgtgacga gccgccggcg ccgcccgagc cgtagcggat cgcgcccggc
     1981 ttggccttgg caagcgcgat cagctccttc agggaactca ccccgagcga cggcgtcacc
     2041 acgtagatga acggctgcgt cgccaccagg gaaaccggca gcagatccct ctcggtgtcg
     2101 tacggcagct tcgagtacag cgccggcgcg tacgacactc cgaggctgca cagcaccatc
     2161 gtgtagccgt ccggcgcgga ctgcaccgcc agctgcgtgc cgacaatgcc gccgctgccc
     2221 ccacggttgt cgaccaccac cgtctgcccc caggtctggg tcagctcctg cgcgacgagc
     2281 cgagcgatga tgtcagtgcc gccgcccggc gcgaacggaa cgatcatgcg caccggcttc
     2341 gatggatagg gttgcgcgaa cgatgtcccc gctgagaatg tcgtcgcggc gaccagcgcg
     2401 atcgcgatcc gcagcaggat gcaacgc
//
LOCUS       DBNGWZ010000241         2941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2292286, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000241 DBNGWZ010000000
VERSION     DBNGWZ010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2292286"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC16CX_07220"
     CDS             <1..688
                     /locus_tag="AC16CX_07220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012341.1"
                     /translation="GPAHVLHGVSLSVHEREVVCLVGRNGAGKTTTMESIMGFLPLSS
                     GQILFHGEDVSSLPVHQRALRGMGYAPEGCAIFPELTVAENMMISRWMSAKAHRRLEA
                     AAAGNIEERVFSVFPEVRDLLRRQGMNLSGGQRKMVAIARGIALAPTLLLLDEAFEGL
                     APIVVKRFRDAVLKIEDLGISLLIAESNLVNAARIADRLYAIDRGEIIFEGKPEQALE
                     DDSVMKALRG"
     gene            826..2469
                     /locus_tag="AC16CX_07225"
     CDS             826..2469
                     /locus_tag="AC16CX_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR3012342.1"
                     /translation="MGYANPYTVGLDKNAANYAPLSPLSLLARTAYVYPKRLALIHGD
                     WQLTWRKVYDRCRKLASALVRAGVKRGDTVAAMLPNVPAMYEMHFGVAMAGAVLNTLN
                     TRLDARAIAFMLDHARAKVVVTDREFSPVMEQALRLAKVKPLVIDADDPEFIGGKLIG
                     KTTYESFIAGGDPDFPWSLPADEWEAISLNYTSGTTGDPKGVVYHHRGAYLNALSNIV
                     SWGMPPHPVYLWTLPMFHCNGWCFTWTVAAAAGTNVCLRRVDARLIFDLIRRHRVTHY
                     CGAPIVHGLLINAPEEWKRGIDHEVSCLVAAAAPPAAIIEGMEKMGFDITHVYGLTET
                     YGPAAVCAKHEEWGSLPLEKRVEMNGRQGVWYHAQECMTVLDPATMAPVPWDGETMGE
                     VMFRGNLTMKGYLKNEKATRAAFAGGWFHTGDLGVMQPDGYVKIKDRSKDIIISGGEN
                     ISSLEVEDVLYRHPAVMAAAVVAQPDSKWGEVPCAFVELKEGVRATEAEIIEHCRSRL
                     AHFKAPRRVVFGTLPKTSTGKIQKFVLRERVKSASAIDP"
     gene            2650..>2941
                     /locus_tag="AC16CX_07230"
     CDS             2650..>2941
                     /locus_tag="AC16CX_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HIR3012343.1"
                     /translation="MTRWTERWVPDAFVIVIILSFVTFIFALIWGFKPEVGLGTRAYE
                     SVQAWGRGFWELLAFAMQMCLIMMTGYILACSPPVRRLLDGLSGWSDPQKPWQ"
BASE COUNT          532 a          927 c          976 g          506 t
ORIGIN      
        1 gggccccgcg cacgtgctgc acggggtctc gctgagcgtt cacgaacgcg aggtggtctg
       61 cctggtcggc cgcaacggcg ccggcaagac caccaccatg gaaagcatca tgggctttct
      121 cccgctcagc tccggccaga tcctgtttca cggcgaggac gtgagctcgt tgcccgtgca
      181 ccagcgggcg ctgcgcggca tgggatacgc gcccgaaggg tgcgcgatct tccccgagct
      241 cacggtggcg gaaaacatga tgatcagccg ctggatgtcg gcgaaggcgc accgccggct
      301 ggaggcggcc gccgccggga acatcgagga gcgtgtattc tcggtgttcc cggaggtgcg
      361 cgacctgctg cgccgccagg ggatgaacct gagcggcggc cagcgcaaga tggtcgccat
      421 cgcccgcggg atcgcgctcg ccccgacgct gctccttctg gatgaggcgt tcgaggggct
      481 tgcgccgatc gtcgtcaagc ggttccgcga cgcggtgctc aagatcgagg acctcggcat
      541 atcgctgctg atcgcggagt cgaacctcgt caatgcggca cgcatcgcgg accggctcta
      601 tgcgatcgac cgcggcgaga tcatcttcga gggcaagccg gagcaggcgc tggaagacga
      661 cagcgtcatg aaagcgctcc gcggctagaa attgccaatc gcgagccaag cttgtagagt
      721 tttcggtgcg gcgcacagtc gccgcacggc cgggaccgtg gcggcccgca cgcgattccg
      781 gcacattcca ccgtgacaac agccactccc ggccgcaggg gtgccatggg ctacgccaat
      841 ccgtacaccg tcggactcga caagaacgcg gcgaactacg cgccgctctc gccgctctcg
      901 ctgctcgcgc gcaccgcata cgtctatccg aagcgccttg cgttgatcca cggcgactgg
      961 caactcacct ggcgtaaggt ttacgaccgc tgccggaaac tcgcttccgc actggtgcgg
     1021 gccggcgtga aacgcggcga cacggtcgcg gcgatgctgc cgaacgtccc ggccatgtac
     1081 gagatgcact tcggcgtcgc gatggcgggc gcggtgctga ataccctcaa cacgcggctc
     1141 gacgccaggg cgatcgcctt catgctcgac cacgcccggg cgaaggtggt ggtcaccgat
     1201 cgggagtttt cccctgtcat ggaacaagcc ctccggctcg ccaaggtcaa gccgctggta
     1261 atcgatgccg atgatcctga attcatcggc ggaaagctga tcggcaaaac cacctacgaa
     1321 tcgttcattg ccggcggtga cccggatttc ccatggtcgt tgcccgccga tgaatgggag
     1381 gcgatctcgc tcaactacac ctccggcacg accggcgatc ccaagggggt cgtctaccac
     1441 caccgcggag cgtacctgaa cgcgctttcc aacatcgtaa gctggggcat gccgccgcac
     1501 ccggtctacc tgtggacgct gccgatgttc cattgcaacg gctggtgctt cacctggacc
     1561 gtcgccgcgg ctgccggcac caacgtctgc ctgcgcaggg tggatgcgag gctcatcttc
     1621 gatctcatcc gcaggcaccg cgtaacccac tattgcggcg cgccgatcgt gcacggcctg
     1681 ctcatcaatg cgccggagga atggaagcgc ggcatcgacc acgaggtttc ctgcctggtc
     1741 gccgcagcgg cgccgcccgc ggcgatcatc gagggcatgg aaaagatggg tttcgacatc
     1801 acgcacgttt acgggcttac cgagacctac gggcccgcgg ccgtgtgcgc caagcacgag
     1861 gaatggggca gcctgccgct cgagaagcgc gtggaaatga acgggcgcca gggggtgtgg
     1921 taccacgccc aggaatgcat gacggtactc gacccggcca cgatggcgcc ggtgccatgg
     1981 gacggcgaaa cgatgggcga agtgatgttc cgcggcaacc tgacgatgaa gggatacctc
     2041 aagaacgaga aggcgacccg ggcggcgttc gcgggcggct ggttccacac cggcgacctc
     2101 ggcgtgatgc agcccgacgg ctatgtcaag atcaaggacc gttcgaagga catcatcatt
     2161 tccggcggcg agaacatttc ctccctggaa gtggaagacg tgctttaccg tcatcccgcg
     2221 gtgatggccg cggcggtcgt cgcccagccc gattcgaagt ggggcgaggt gccgtgcgcc
     2281 ttcgtcgagc tcaaggaggg tgtgcgggcg accgaagcag agatcatcga gcattgccgc
     2341 agccgcctcg cgcatttcaa ggcgcccagg cgcgtggtgt tcgggacgct tcccaagacc
     2401 tccaccggca agatccagaa attcgtcctg cgcgagcggg taaaatcggc ctccgccatc
     2461 gatccctagc gccggtgaaa gagaatcttt cccgcttgac ctgaggggga gaagggtgga
     2521 acctgttatg tcggcggcat catgctgatg cctgcctgag cggtcggaaa gggggaatgt
     2581 atggcgaacg gggcaaggaa ggaggaaggt cgcggatccc ttgagttcct gaaagactgg
     2641 ggtgggggca tgacccggtg gacggagcgg tgggtcccgg atgcattcgt cattgtcatc
     2701 atcctgagct ttgtcacctt cattttcgcg ctcatctggg gattcaaacc ggaggtcggt
     2761 ctgggtaccc gggcctacga atcggtccag gcatggggaa ggggcttctg ggaactgctc
     2821 gccttcgcca tgcagatgtg tctcatcatg atgacgggct atatcctcgc ctgctcccct
     2881 ccagtccggc ggttgctcga cgggctctcc ggctggtccg atccgcaaaa gccctggcag
     2941 g
//
LOCUS       DBNGWZ010000242         9563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1032875, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000242 DBNGWZ010000000
VERSION     DBNGWZ010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1032875"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="AC16CX_07235"
     CDS             complement(<1..1872)
                     /locus_tag="AC16CX_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012344.1"
                     /translation="MTGARSTTHDPRPTIFSGRWPLLHALILGPFRENPGRSVLAIAA
                     IALGVALGVAVHLVNSSALNEFELAARHLAGEADLVVRGPRAGFDEALYPKLARLPQV
                     EAANPAVDLEAPLAGRRDSLRIIGFDPLRAAQVQPALLPERSGMVLDLFESDAILLSP
                     SAAEWLRLKAGDGLDIQVGTRTISLRIVGLLPQGAYRQRLGVMDVSSAQWRLAQLGRL
                     NRIDLRLKPGTDVAAFRRELQAILPAGVHAVTPETEGERSANLTRAYRLNLDMLALVA
                     LFTGAFLVFSSQVLALLRRRAQFALLRAIGVTRRELAARLIAEGAVIGVIGSVLGALL
                     GYAVAHYALLLIGADLGAGYFRSIAASLDAEPRVLAFFVALGVLYAVLGAAAPAWEAA
                     RRPPAQALRAGDEEEGLRKLATLWWGIASIAAGLLLALAPAINGLPVAGYVAIGLILL
                     GTVLVMPRLAGTCLGLLPLPEPPPAAIAIAQLKATPRQAAISVAAIVISFSLMVSMLI
                     MVSSFRGSLEAWLERMLPADLYLRAARFGETAFFTPEEQARIAATPGIERVAFIRSQN
                     LLLAPERPPVTLLAGPVEAGEPGKVLPLTSPPYAVRPDSPPPVWVSEVAADFYSLRVG
                     "
     gene            complement(1896..2561)
                     /locus_tag="AC16CX_07240"
     CDS             complement(1896..2561)
                     /locus_tag="AC16CX_07240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012345.1"
                     /translation="MLSISNLCKSYGGVRPREVLRDISLEVRRGEYVAIVGESGVGKS
                     TLLNLIAGLDLPDHGSIALDGAELTSLDDVSRTLLRRSRIGFVFQAFHLLPHLTVARN
                     VALPLSLNEVDPAQASERVAAMLTAVGLAAAADAYPRELSGGELQRVAIARALVHRPL
                     LLLADEPTGNLDPETAIQVLTLLRDQVKRESGAGILVTHSAIAAATTDRVLTLTADGL
                     CPR"
     gene            complement(2646..3563)
                     /locus_tag="AC16CX_07245"
     CDS             complement(2646..3563)
                     /locus_tag="AC16CX_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012346.1"
                     /translation="MSHAVAAQTYPVKPIRILVPTPPGGGNDIMARLAGQKLNERWGQ
                     PVVVDNRPGAGGQIAMETSARAAPDGYTLLLGTTNLTVLPSMARVAYDPVRDFIPVSL
                     MATSANLLLVHPGVEVRSLQQLLALARAKPGQLNYASGSVASSTQLAAELMKSMARVN
                     ITHVPYKGAGPALVDLLAGQVQIMFSNPAASAAHVRSGKLRALAVSSEKRLPDFPDLP
                     TMAESGLPGYEASTWWGILAPRGTPSSIVAQLNGVISSAITAGDVRSRIAALGAQPAG
                     GPPQQLAILIRDEIPKWARVIKEAGIRLD"
     gene            complement(3675..4931)
                     /locus_tag="AC16CX_07250"
     CDS             complement(3675..4931)
                     /locus_tag="AC16CX_07250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HIR3012347.1"
                     /translation="MSDSPALVRFTGALGTRLAARLDSPPGKPTAYALFAHCFTCSKD
                     TLAASRISAALTARGIAVLRFDFTGLGSSEGDFANTNFSSNIADLIAAAAWLREQHQA
                     PRLLIGHSLGGAAVLAAAHEIPEAVAVATIGAPYEPAHVKRLLAPALPEIEAAGEAEV
                     ELAGRKFTIRKQFLEDIGSRNSREAIGNLRKALLIFHSPRDMTVSIDNAAQIFMAARH
                     PKSFVSLDDADHLLTRQEDAVYVAAVLAAWASRYLGEPIMESRPIAPGDPGVITVTET
                     REGKFTQAIQAGKHRLRADEPSGVGGTDTGPSPYDLLLAGLGACTSMTIRMYADLKQL
                     PLDKVTVRLKHEKVHAEDCAECETKEGKIDRIEREIELEGGLDEASRARLLEIANKCP
                     VHRTLHSEVWIPTRLRNSPDVVPANS"
     gene            5075..6061
                     /locus_tag="AC16CX_07255"
     CDS             5075..6061
                     /locus_tag="AC16CX_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012348.1"
                     /translation="MKRLQGKSCWLLACALVIAFLASRSGAQGYPARPVRIIVPFAAG
                     GAADVPGRILAARLSEALRQQFVIDNRPGAGGTIGADLAAKAAPDGYTLLLISSTHLV
                     SATLHSKRIQYDPVNDFAQITEFGSAPNVLAVHPSLPAKSVKQLIALARTQPGKIDFA
                     SSGNGGSQHLFGALFISMAGIKLTHIPYKGSATAAADLVAGQVPVGFPGIAVALPHVK
                     AGRLRALGLTSAKRSVAMPDVPTIAEAGVPGYEATLWLGLAAPRNTPRDIIDTLHGET
                     VKALKLPELQKSFIASGTEVSYQDTPDQFTAYVKSEAVKWAKVVQLSGAQVN"
     gene            6091..7068
                     /locus_tag="AC16CX_07260"
     CDS             6091..7068
                     /locus_tag="AC16CX_07260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012349.1"
                     /translation="MKAIVWKCVVLLSCGIVLSAGLGTAQGQAYPSKPIRVIIPFGAG
                     GSTDVLVRIVATRMPEALGQQVVIDNRTGAGGLIGTDIAAKSNPDGYTLLATGSPHAI
                     FPNLYKNVPFHPLNDFAPIMQIASQPYGLAVHPSLGVRTVKELIALAKKEPGKHNYAS
                     SGQGGAMHLFQALFANMANIDIVHVPYKGSGPVRADLLGGQVKIGCLGLSSIINNHKA
                     GQLRIIAVTTAKRSPELPDVPSIAETLPGYDAALWTGFLAPRGTPAVAIKRIQSELTK
                     LLQTPEVRNAFQRAGSDIVVTDPKAFGEFLRVEYAKWGKVVRDLKLQVN"
     gene            complement(7313..8860)
                     /locus_tag="AC16CX_07265"
     CDS             complement(7313..8860)
                     /locus_tag="AC16CX_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3012350.1"
                     /translation="MLHDGGEIALLDVREAGQFGESHLLFATPLPYSRLELDIGALVP
                     RKSARIVVCDDGTLGVADRAAKRLRALGHTDVSVLDGGTKAWAAAGYALFAGVNVPSK
                     AFGKLVEHAYHTPRVTSQELARMRAAGEDFVILDGRPFNEYHKMNIPGGICCPNAELP
                     YRALSIIRNPKTKIVVNCAGRTRSIIGAQTLINFGVPNPVVALENGTQGWVLADFNLE
                     HSATRKYPDPIDASLLPAQQASARRLMERFAITTASPAEVTAWLKDTHRTTYLCDVRT
                     PEEFTSGSIPGAVHAPGGQLIQATDQWVGVRNARIVLFDGGENVRAPMVASWLKQLGS
                     DAYVLKGGVRSGLKGVPAPKPALPALDVIPAPELKRALDTGACAVFDLVPSMSFRKAH
                     IPGSHWSARVRLAADASEEKGLIVLVAEDADVARLAALELFEAGAGKVKLLEGGLSAW
                     KHAGYRTEATPEMPPDTDCIDHLFFVHDRHAGNREAMKQYLAWETGLVAQLDEQDRDL
                     FRIATAR"
     gene            complement(9160..>9563)
                     /locus_tag="AC16CX_07270"
     CDS             complement(9160..>9563)
                     /locus_tag="AC16CX_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3012351.1"
                     /translation="DYVHRIGRTGRAGLPGEAISLVSPDEMKFLEDIERLLKRKLPRA
                     PAPESLPAGRHAPHARGARHEREARQIHQSRPQYAPKPSTPAFDFTKPYEPSSPATAP
                     RTSAAPVTHRKPHRPTAALLGGGPAKREPHK"
BASE COUNT         1633 a         3067 c         3250 g         1613 t
ORIGIN      
        1 gccgacccgc aggctgtaga agtctgcggc gacctcggac acccacaccg gcggcgggct
       61 gtcagggcgc accgcgtagg gcgggctggt gagcggcagc acttttcccg gctcgccggc
      121 ctcgaccgga cccgcgagga gggtgaccgg tgggcgttcc ggcgcgagca gcaggttctg
      181 gctgcggatg aatgcgacgc gctcgatgcc cggagtcgcg gcgatgcgcg cctgttcctc
      241 gggcgtgaag aaagcggtct cgccgaagcg cgccgcgcgc aggtagaggt cggcgggcag
      301 catgcgctcg agccaggctt cgagcgagcc gcggaacgag ctcaccatga tcagcatcga
      361 aaccatgagg ctgaagctga tgacgatcgc cgcgacgctg atcgccgcct ggcgcggtgt
      421 cgccttgagc tgcgcgattg cgatcgccgc cggcggaggc tcgggcaggg gcagcagacc
      481 gaggcacgtc ccggcgagcc gcggcatgac gagtaccgtc ccgagcagga tcaggccgat
      541 ggcgacatag ccggcgaccg gtagaccgtt gatggccggg gcgagcgcga gcaacagtcc
      601 cgccgcgatc gaggcgatgc cccaccacag tgtggcgagc ttcctgagcc cctcttcctc
      661 gtcaccggcg cgcagcgcct gcgcgggagg gcggcgtgcg gcctcccacg ccggtgcagc
      721 ggcgcccagc accgcgtaaa gcacgccaag cgcgacgaag aaggccagca cccgcggttc
      781 ggcatccagg cttgcggcga tgcttcggaa atagccggcg ccgaggtcgg ctccgatcag
      841 cagcagcgcg taatgcgcaa ctgcgtagcc gagcagcgct ccgagcaccg atccgatgac
      901 gccgatcacg gcgccttcgg cgatgagccg ggcggccagt tcccgacggg tgacgcctat
      961 tgcgcgcagc agcgcgaatt gcgcgcgccg gcgcagcaag gccagcacct gtgacgagaa
     1021 caccaggaac gcgccggtga agagcgcgac cagcgctagc atgtcgaggt tgaggcgata
     1081 ggcgcgcgtc aggttggcgc tgcgctcgcc ctcggtttcc ggcgtcaccg cgtgaacgcc
     1141 ggcaggcagg atcgcctgga gctcgcggcg gaaggccgcg acgtccgtgc cgggcttgag
     1201 gcgcagatcg atgcggttga gccgtcccag ctgcgcaagg cgccactggg cggaagagac
     1261 gtccatcacc cccaggcgct ggcggtaggc cccctgcggc agcaggccga cgatcctgag
     1321 cgaaatggtg cgcgtaccga cctgtatgtc gaggccgtcg cccgccttca gccgcagcca
     1381 ctccgcggcg gacgggctca gcagtatcgc atcggactcg aagaggtcga gcaccatgcc
     1441 ggagcgctcc ggcaacagcg cgggctgcac ctgtgccgcg cgcagcggat cgaaaccgat
     1501 gatcctcagg ctgtcccggc gtccggcgag cggcgcctcg aggtccaccg cgggattggc
     1561 cgcctcgacc tgcggcagcc gcgcgagctt tggatagagc gcctcgtcga aacccgcccg
     1621 cgggccgcgc accaccaggt cggcctcgcc cgcaagatgc cgggccgcga gctcgaattc
     1681 attgagcgcg ctggaattga cgaggtgaac cgcgacgccg agggcaacgc ccagagcgat
     1741 cgcggcgatc gccagcacgg aacgtcccgg gttttcgcgg aacggcccca ggataagcgc
     1801 gtgcagcaga ggccaccggc cgctgaagat cgtgggtcgt gggtcgtggg tcgtggatcg
     1861 ggcaccagtc acgagtcacc agtcacgagt cacgctcagc gcggacagag gccgtccgcg
     1921 gtgagcgtca gcacccggtc ggtcgtggcg gccgcaatcg ccgaatgagt gacgagtatg
     1981 ccggcgccgg attcacgctt gacctgatcg cgcagcagcg tcaacacctg aatcgcggtt
     2041 tcgggatcga gattgccggt cggctcgtcc gcgagcagca gcagcggccg gtgcaccagc
     2101 gcccgcgcaa tcgccacgcg ctgcagctcg cctccggaga gttcccgggg ataggcgtcg
     2161 gccgcggcgg cgagccccac cgcggtcagc atggcggcga cccgttcgct tgcctgcgca
     2221 ggatcgactt cattgaggga cagggggagg gcgacgttcc gggccacggt caggtgcggc
     2281 aacaggtgga atgcctggaa cacgaagccg atgcggctgc gccgcagcag cgtgcgcgag
     2341 acatcgtcca gcgacgtcag ctccgcgcca tccagggcga tgctgccgtg gtcgggcaga
     2401 tcgagccctg ctatgaggtt gagcagcgtg gacttgccga cccccgactc cccgacgatg
     2461 gcgacgtatt cgccgcgccg cacctcgagc gagatgtcgc gcagcacctc gcgcggccgg
     2521 acgccgccgt aggacttgca gagattgctg atgctcagca caagcgttca gctatcagct
     2581 gtcagcgatc agcttaaacc aaatataacg gtctggctga ccgctgaaag ctgacggcgc
     2641 cggtctcagt cgagtctgat accggcttcc ttgatgacgc gcgcccactt gggaatttcg
     2701 tcgcgaatca ggatagccag ttgctgcggc ggcccgccgg cgggctgcgc gccaagcgcc
     2761 gcaatccggc tgcgtacgtc gcccgcagta atggcgctcg agatgacccc gttcaactgc
     2821 gcgacgatgg acgacggggt gccgcgcggc gcgagtattc cccaccacgt ggaagcctcg
     2881 taaccgggga gccccgattc cgccatggtc ggcagatccg ggaaatcggg caggcgcttc
     2941 tcgcttgaca ccgcgagcgc gcgcagctta ccggagcgca catgcgcggc ggatgcggcg
     3001 ggattgctga acatgatctg cacctggccg gcgagaaggt ccaccaaggc ggggcctgcg
     3061 cccttgtagg ggacatgagt gatattcacg cgcgccatgg atttcatcag ttccgcggcg
     3121 agctgggtcg aggacgcgac gctgcccgat gcgtagttga gctgcccggg tttcgcccgc
     3181 gccagcgcca gcaactgctg cagcgatctc acttcaaccc ccggatgaac gagcagcaga
     3241 tttgcggagg tggccatcag cgagaccgga atgaaatcgc gcactgggtc gtaggcaacc
     3301 ctggccatgc tgggcagcac cgtgaggttg gtggtgccga ggagcagtgt atagccgtcg
     3361 ggcgcggccc gcgccgaggt ctccatggcg atctgcccgc ccgcgccggg gcggttgtcc
     3421 accaccaccg gctggcccca gcgctcgttc agtttctgcc ccgccagccg cgccatgata
     3481 tcgttgccgc cgccgggcgg cgtcggcacc agtatgcgta tcggcttgac cggataggtt
     3541 tgagctgcta ctgcgtggct caaacctatc aataggaccc cgacagaaat agctgcgacg
     3601 ggtttcatat gtcctccatt ccctgtaatc gatagattca aacccgagcc cggcctttca
     3661 ttttgctgcg cgtattagga attcgccgga accacatccg gcgaattcct cagccgcgtc
     3721 ggaatccaga cctcggaatg cagcgtgcgg tgcaccgggc acttgttggc gatctcgagc
     3781 aggcgcgcgc ggctcgcctc atcgagaccg ccctcgagct cgatctcgcg ctcgatgcgg
     3841 tcgatctttc cctccttcgt ttcgcactcg gcgcaatcct cggcgtgcac cttttcgtgc
     3901 ttgaggcgca ccgtcacctt gtcgagcggc agctgcttca ggtcggcgta catgcggatc
     3961 gtcatcgagg tgcaggcgcc gagcccggcg agcagcaggt cgtagggtga agggccggta
     4021 tccgttccgc cgacgcccga cggctcgtcg gcgcgcaggc ggtgcttgcc cgcctgtatc
     4081 gcctgcgtga atttgccttc gcgcgtttcg gttaccgtga tgacgccggg gtctccgggc
     4141 gcgataggcc gggattccat gatcggctcg ccgagatagc ggctcgccca ggccgcgagc
     4201 actgccgcga cgtagacggc gtcttcctgg cgcgtgagca agtgatcggc gtcgtccagc
     4261 gaaacgaagc tcttgggatg cctggcggcc atgaagatct gcgccgcgtt gtcgatgctt
     4321 accgtcatgt cgcgcgggga atggaagatc agcagcgcct tgcgcagatt tccgatcgcc
     4381 tcacggctgt tccggctgcc gatgtcctcg aggaactgtt tcctgattgt gaacttgcgg
     4441 ccggcaagct ccacctccgc ttcgcccgcg gcctcgatct cgggcagcgc cggagcgagc
     4501 aggcgtttta cgtgggcggg ctcgtacggc gcgccgatgg tcgcaacggc cactgcttcc
     4561 ggaatctcat gcgctgcggc aagcactgcg gcgccgccca ggctgtgacc gatcagcagc
     4621 ctgggcgctt gatgctgctc ccgcagccac gcggcggcgg cgatcaggtc ggcgatgttc
     4681 gatgaaaagt tggtattggc gaaatcgcct tcgctgctcc cgagtccagt gaagtcgaag
     4741 cgcagcaccg cgatgccgcg cgcggtgagc gccgcgctga tgcgggaggc ggccaacgtg
     4801 tccttcgagc aggtgaagca gtgcgcaaac agcgcgtagg cggtgggctt gccgggcggg
     4861 gaatcgaggc gggcggcaag ccgggttccg agggcgcccg tgaaacggac gagggcgggg
     4921 ctgtcgctca tgtggcctcc tcaggacatt gcgtctgatg gtagcatgcg aacgacgacg
     4981 catacgacgg ggacccggcc cggtttcaag gttctcaagg gtcaattgtt atgatggttc
     5041 gatcggatgg agcgcgtccg tcaggaggga attcatgaag cgtctgcagg gtaaatcctg
     5101 ttggctgctg gcgtgcgcgc tggtgatcgc gtttctcgcc tcccggtcgg gcgcccaggg
     5161 atatccggcg cggccggttc gcatcatcgt gccattcgct gccggcggcg cggctgacgt
     5221 gccgggacgc atcctggcgg cgaggctgtc cgaggcgctg cgccagcagt tcgtgatcga
     5281 caaccggccc ggggcgggcg gcacgatcgg cgctgacctc gcggcgaagg ccgcccccga
     5341 cggttacacg ctgctcctca tcagcagcac gcatctcgtg agcgccacgc tgcacagcaa
     5401 gcgcatccag tatgacccgg tcaacgattt cgcgcagatc accgaattcg gcagcgcgcc
     5461 caacgtgctc gcagtccacc cgtcgctgcc cgcgaaatcg gtgaagcagc tgatcgcgct
     5521 cgccaggacg cagcccggca agatcgattt cgcttcctca ggcaacggtg gttcgcagca
     5581 cctgttcggc gcgcttttca tctcgatggc cgggatcaag ctgacgcata tcccgtacaa
     5641 gggcagcgcc acggcggcgg ccgacctcgt cgcggggcag gtgcccgtgg gctttcccgg
     5701 aatcgccgtc gcgttgccgc acgtgaaggc cggaaggctg cgcgcgctcg gcttgaccag
     5761 cgcgaagcga tcggtcgcca tgccggacgt cccgacgatc gcggaagcgg gtgttcccgg
     5821 ttacgaggcg acgttatggc tggggctcgc tgcgccccgg aacaccccgc gcgacatcat
     5881 cgacacgctg cacggcgaga ccgtgaaggc gctcaagctg cccgagctgc agaagagctt
     5941 catcgcgagc ggaaccgagg tgagctacca ggataccccc gatcaattta ccgcctacgt
     6001 gaaaagcgag gcggtcaagt gggcgaaagt cgtgcaactc agcggcgccc aggtcaacta
     6061 agggctctat caataacgag gaggaggcaa atgaaggcta tcgtgtggaa atgcgtggtt
     6121 ttattgtcgt gcgggatcgt cctgtcagca gggctgggaa ccgcgcaggg gcaagcctat
     6181 cccagcaagc cgatccgcgt aatcattccg ttcggcgcgg gtggctcgac cgatgtgctc
     6241 gtgcgcatcg tcgcgacgag gatgccggaa gccctgggcc agcaagtggt gatcgacaat
     6301 cgcacgggtg ccggcggcct gatcggaact gacatcgcgg cgaagtcgaa tcccgacggc
     6361 tacacgctgc ttgccacggg ttcaccgcac gccatctttc ctaatctgta caagaacgtg
     6421 ccgttccatc cgctgaacga ctttgcgccg atcatgcaga tcgccagcca gccgtacgga
     6481 ctagcggtgc atccctcgct tggcgtcagg acggtgaagg agctcattgc gctagcgaaa
     6541 aaggagcctg gcaagcacaa ctacgcttcc tcgggccaag gaggcgcgat gcacctgttc
     6601 caggcgttgt tcgccaacat ggcgaacatt gacatcgtgc acgtcccgta taagggcagc
     6661 ggtccggtgc gtgccgacct gctgggcggc caagtcaaga tcggatgcct cggcctctcg
     6721 agcatcatca acaatcacaa ggcgggacag ctgcgcatca tcgcggtgac gaccgccaag
     6781 cgctcaccag agctgccgga cgttccgtcc attgcggaaa ccctgccggg ctacgatgcg
     6841 gcgctgtgga cgggatttct cgcgccgcga ggaaccccgg cggtagccat caagcgcatc
     6901 cagagcgagc tgacgaaatt gctccagacg ccggaggtgc ggaacgcgtt ccagcgggcg
     6961 ggttccgaca tcgtggtcac cgatccgaag gccttcggcg aatttctccg ggtcgagtac
     7021 gccaagtggg gcaaggtcgt gcgcgacttg aagctgcagg ttaattgatg ctgggatgtg
     7081 acgaataagg gtgacgaata agggtgacga tcaaaggtga cgaagggcag tagcggcttg
     7141 tacgtcaccc gtatctcgtc accctgcggc ctctgaaatc ccatcgcgag aattcgaggt
     7201 gatcacttcg gccgccccgc gcgtcaccgg gggtcacatc cccgaacggg ttcggtgccc
     7261 ctgttccgcc cctcgacgcg cctcgtcacc attatttgtc acgcacagtg cgctatcgcg
     7321 cggtggcgat cctgaacaaa tccctgtcct gttcgtcgag ctgggcgaca agcccggtct
     7381 cccacgcgag gtattgcttc atcgcctcgc gattgccggc gtggcggtcg tggacgaaga
     7441 agagatggtc gatgcagtcg gtgtccggcg gcatttccgg cgtggcctcg gtcctgtagc
     7501 ccgcgtgttt ccacgcgctc agcccgcctt cgagcaactt gactttcccg gcaccggcct
     7561 cgaagagctc aagggcggcc aggcgcgcga catcggcgtc ttctgccacc agcacaatca
     7621 agcctttttc ttcactcgcg tccgccgcaa gccgcacgcg cgcgctccaa tggctgccgg
     7681 gtatatgagc cttgcggaag ctcatgcttg ggacgagatc gaacacggcg caggcgcccg
     7741 tgtcgagcgc acgcttgagt tcaggggccg ggatcacatc gagcgccgga agagcgggtt
     7801 taggcgccgg cacacccttg aggcctgagc ggacgccgcc cttgaggaca taggcgtcac
     7861 taccgagttg cttcaaccag cttgcgacca tcggcgcgcg gacgttctcg ccaccgtcaa
     7921 acagcacgat acgcgcgttg cgcaccccga cccactggtc ggtcgcctgg atcaactggc
     7981 cgccgggtgc gtggaccgcg ccggggatgg aacccgatgt gaattcctcc ggcgtgcgca
     8041 cgtcgcagag gtaggtagtg cgatgcgtgt ctttcagcca ggctgtgacc tctgcggggg
     8101 aggcggtcgt gatcgcgaat cgctccatga gtctcctcgc agacgcctgc tgcgcgggca
     8161 ggagcgacgc gtctatcgga tccgggtact tgcgggtggc gctgtgctcc agattgaagt
     8221 cggccaggac ccatccctgc gtcccgtttt ccagcgcaac gaccgggttc gggacaccga
     8281 agttgatcag cgtctgcgcg ccgatgatgc tgcgggtgcg gccggcgcag ttcaccacga
     8341 ttttcgtctt cgggttcctg atgatgctca aggcacggta gggcagttcc gcattgggac
     8401 agcagatgcc gcccgggatg ttcatcttgt ggtattcgtt gaatggccgg ccgtcgagaa
     8461 tcacgaaatc ctctccggcg gcacgcatgc gggcgagttc ctgcgacgtt acgcgcggcg
     8521 tgtgataggc gtgctcgacc agcttgccga acgccttgct cggaacgttg acgccggcaa
     8581 agagcgcgta gcccgcggcg gcccacgcct tcgtgccgcc gtcgagcact gagacgtccg
     8641 tatgacccag cgcccgaagc cgcttcgcgg cgcggtccgc cacgccgagc gttccgtcat
     8701 cgcagacgac gatcctggcg gacttcctgg gcaccagcgc gccgatgtcg agttcgagcc
     8761 ggctgtacgg caacggcgtg gcgaacagca agtgcgactc gccgaactgt ccggcttcgc
     8821 gcacgtcgag cagggcgatc tccccgccgt cgtgcagcat ttctttgagc gcggaggtga
     8881 cgacggtttt cacagatgca ccacgacggt catgtgagag ttcttgcacc gtagtgttca
     8941 gcggtcagca ttcagccccc agctttaagc aataacggtc ccggctgata gctgatcgcg
     9001 agcgcgcgat tgcgcgtgcc ccaagtttag cgcagtacca acggcacgcc gctcgcgatc
     9061 agcaccacgg ccacgaaacg cctgaaagtg atagggagta agcaacgccc gcttcgcggg
     9121 aaacttatca cttatcactc gtcacttatc acaccgtagc tatttgtgag gctcgcgctt
     9181 cgcggggccg ccgccgagga gggcggcggt ggggcggtgt ggctttctgt gggtgacggg
     9241 tgcggcggat gtgcgcgggg ccgtagccgg tgatgaaggt tcatacggct tggtgaagtc
     9301 gaaggcgggc gtcgaaggct tgggggcgta ctgcggcctg gactggtgaa tttgccgcgc
     9361 ctcgcgctca tggcgcgctc cccgagcatg cggcgcgtgc cggcctgcgg gaagcgattc
     9421 cggcgccgga gcacggggca gcttgcgctt gagcagccgc tcgatatcct cgaggaattt
     9481 catctcgtcg gggctgacca gtgagatggc ctcgcccggg agcccagccc gtccggtgcg
     9541 gccgatgcgg tgcacgtaat ctt
//
LOCUS       DBNGWZ010000243        10855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1157811, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000243 DBNGWZ010000000
VERSION     DBNGWZ010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1157811"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="AC16CX_07275"
     CDS             complement(<1..913)
                     /locus_tag="AC16CX_07275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3012352.1"
                     /translation="MQTRVGKYELKKELGAGASGKVYLALDTYAGKDVALKVIDSQVL
                     RDSEVGELTRGQFMNEATLAGKLAHPHIVSILEAVMEEDSSYVAMEYVPGGSLAKFIK
                     PEGLLPMEDVIQIGFKFCSALDYAFRQGIVHRDIKPANILVTQGTNIKVADFGAALLK
                     STQSTQDLLVGTPSYMSPEQISGAPLTHHSDMYAMGVVFYELLTGQRPFTASSLTALF
                     QKIMEEEPALPSVLRSSLSAKLDPIILRVLSKRPEDRYPTWADLALDIAQVGKLSVYQ
                     REVPDSDKFGVLRKSELFKQINDAQVWE"
     gene            complement(1213..2145)
                     /locus_tag="AC16CX_07280"
     CDS             complement(1213..2145)
                     /locus_tag="AC16CX_07280"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3012353.1"
                     /translation="MKIAIIGQQDFGKAVLEAFLARKDEVTGVFCAPEKAGAKADLLR
                     TAAEEKGLKVFQFASLRSPEAHDAMTGLKVDLGIMAYVLQFAPDSMVKIPRLGTVQYH
                     PSLLPKYRGPSSINWPIIRGDTRTGLTIFRPTEGLDEGPIVLQRECDIGPDETIGDVY
                     FNKLFPMGVKAMLEAADLVFAGKHREIAQDESKASYEGWCREAEARIHWAHHVDFVYN
                     IIRGCNPAPGAWTTLNGKKLQIFDARKHLFRRFADVVGKIGEVSEVTATSFKVTAQGG
                     LIEVLRARGEDGKKLSGGDYARATGLAAGIILGN"
     gene            complement(2260..3531)
                     /gene="frc"
                     /locus_tag="AC16CX_07285"
     CDS             complement(2260..3531)
                     /gene="frc"
                     /locus_tag="AC16CX_07285"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HIR3012354.1"
                     /translation="MEALKGIRILDMTHVQAGPTCSQLLAWMGADVIKFENPQGDATR
                     GQLRDVPNADSLYFTMLNCNKRSITVNMKTAEGKVVFVDLLKKCDIVMENFGPGVLDR
                     FGFPWEKIREINPRIVMGSIKGFGSSGPYANFKAYENVAQAMGGAMSTTGVPDGPPFV
                     TGAQIGDTGTGLHLAIGLLAALRQRDRTGKGQYVEVAMMDGIMNLCRVKFRDHQRLTR
                     GGLSEYSVPTHKGMGDVPRGGNDSGGGQLGNAVRCKPGGTNDFIYIVVQEAVWDALAR
                     RISVELGLPGLVTDPKFATIDERRRNQADMWELIGEFALKHDKREFMQILNELDVPCG
                     PVMGTEDITHDEHVRGRQMWVELDHPQRGKWYNVGMPIKLSDSPPEIKRSPLLGEHTD
                     EILREVLGYNNERIAQLKQAGAFSAPPKKAA"
     gene            complement(3593..5311)
                     /gene="oxc"
                     /locus_tag="AC16CX_07290"
     CDS             complement(3593..5311)
                     /gene="oxc"
                     /locus_tag="AC16CX_07290"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HIR3012355.1"
                     /translation="MQLVQEVTDGFQLVIDTLKLNGIDTIFGLPGIPITDLTRRAQAE
                     GMRVISFRHEQNAGNAAAIAGFMTQKPGICLTVSAPGFLNGLTALANATTNCFPMILI
                     SGSSEREIVDLQQGDYEEMDQLAIAKPLCKAAFRVLHAQDIGVGIARAIRAAVSGRPG
                     GVYLDLPAKLFAQTLDAEAGRKSLIKVVDPAPRQIPSRDAVQRALELLKSAKRPLIIL
                     GKGAAYAQADADIRALVEKTGIPYLPMSMAKGLLPDTHEQSAAAARSYVLAEADVVML
                     IGARLNWLLSHGKGKTWGGKDHKDWGGQKFIQIDISPQEMDSNVRIHAPLVGDIGSCV
                     SALLAGIGSNWAKPPADWLRAIAERKNKNLAKMAETLAKDPRPMNFHSALNVVRDIIK
                     ANPEAILVNEGANALDFTRSIVDMYQPRKRLDVGTWGVMGIGMGFSVAAAVVSGQPVI
                     AIEGDSAFGFSGMEVETICRYNLPVCVVIFNNNGVYRGTDVNPTGGPDVAPTVFVKGA
                     RYDKLIEAFGGVGVHATTPAELRKAMEEAIRSRKPTLINAVIDETAGTESGRITSLNP
                     TSGKKK"
     gene            5517..6125
                     /locus_tag="AC16CX_07295"
     CDS             5517..6125
                     /locus_tag="AC16CX_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3012356.1"
                     /translation="MPVLAQHPEAKIIRLQLRQNRALKHLDAAQWRELDPLLEIADYA
                     KGESLEHQGDWSMEQYFVIDGILKRVVSNAQGKEMILRFAAEADIDTSYAAWRLKTPI
                     PYSIRAVTKVRAAKLSMPNWVQFLERHPAIKSAFEFEIMKLMSEVMAHTITLHLLDAP
                     GRVQRFMRKHSELADRLPKKELACYLNLSPETLSRLKRRGKI"
     gene            complement(6415..7434)
                     /locus_tag="AC16CX_07300"
     CDS             complement(6415..7434)
                     /locus_tag="AC16CX_07300"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012357.1"
                     /translation="MEETKLKADVGDFTSPQVLDPWKGIKKVAREEYYTSGHRTCQGC
                     ESALVMRLMIKAAGPRTIVLGATGCMYVANTTYYSTPWVVPWMHTQLGSAGSGATGTA
                     AALRALMRKGKMSKEPINVIAFCGDGGGADMGLSAISAALQHMQYNLLIFLYDNESYA
                     NTDIQVSGSSPYGANTTFSPPGKVHRIMHRRWKKNTAAMLAAGHPDCKYVATVCSGNA
                     VDTLNKVRRALSIGGPTFIHSLDPCPKGWDYDPKYSHELGELAVETGIWPLYEVEDRK
                     LKMYGKSKHIASGAAKRKPVGEYLLRQGRFAHFTEEDIAYFQAKTDEMWEKWLIPGVI
                     PFAQG"
     gene            complement(7440..8618)
                     /locus_tag="AC16CX_07305"
     CDS             complement(7440..8618)
                     /locus_tag="AC16CX_07305"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012358.1"
                     /translation="MISGSEAVAHAVRLADVDVITAYPIRPYDTVMQYVSKMIADGDL
                     DCEFIVAESEHSQFEIVKHASAVGARVFVGSSGVGWFYAFEALAVTAGLRLPMVAMVG
                     NRALDDPGAFGVEHNDALAVRDLGWMLCWVSTAQEALDTALIAYRVAEDPRVFLPCAI
                     SCDGAFLTHSQSIVKVPSQAMVNSFLPRYNRGDKQLHPDNPITIAPQVNEDWLMEMRR
                     QSDMAARRARDVIIEAYDDFNAKFGRGGEPFIERYMMEDAEVAVVGMGTLAMPVKVAV
                     RKMREQGKKVGFVRVRWFRPFPTRELQDALAGVRAVGVIDRDFSFGSTFFSGVLANDL
                     RSALYPVKNRPKILSFIAGLGGREVRTNDVIDVTSKVFDAARGADIDEQNTYWIGVRE
                     "
     gene            complement(8677..9819)
                     /locus_tag="AC16CX_07310"
     CDS             complement(8677..9819)
                     /locus_tag="AC16CX_07310"
                     /inference="COORDINATES: protein motif:HMM:NF026048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HIR3012359.1"
                     /translation="MSTGSVEVVYRGIFQKTLAKNICRGIVFAARKEGKIGIAFGRYG
                     DSPERNGIPAKQFAVVADTEEEMEEYLSRYEPDNNDVTIVADDTLCKGIESWAWYGLQ
                     PINKLLKEGGTLLVTSTRSPESLIGDIHRKGKPYHLAIVKASASFSGLWVYKDDHTDV
                     RLLGALARVAPHIISLDSIVAAINEPKKNAAKEASARKAHDLIASTAIGPDNGNDAVP
                     FKFELPGWKKMEECLVIRGVGLKQEVPGRDGGYVPARNPTFKKFTTRTMRPVINFDTC
                     TKCTICWLQCPDTCFDITPDGYYDANMEACCGCGICEAVCPVDKCITMVNEEAFKDHR
                     SQWEMWHKDKQAYAAWLSEKVADQPARRHGFHHLGQYAEEIANEPS"
     gene            complement(10025..>10855)
                     /locus_tag="AC16CX_07315"
     CDS             complement(10025..>10855)
                     /locus_tag="AC16CX_07315"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase domain-containing
                     protein"
                     /protein_id="HIR3012360.1"
                     /translation="LEVLAESRIRDIYMIGRRGPAQAKFTHQELRELGNLADCDPVVD
                     PRDLDLNPASREELADRRNRANIKSYEVLQGFASRPPPTRHRRCHIQFLKSPKELKGT
                     GRLERVVLMRNRLEGEPSQQVARETGDVEELACGVLFRSIGYQGVPIAGVPFDDGRGI
                     FPSRDGRIVDGQNVVPGLYVAGWIKRGPTGIIGTNREDSVATVNSILADLPNLSAGAK
                     TGADGLTALLQSRTVRVVSYADWQKIDAAEVRRGATAGKPREKFTRVEEMLEVVGHSE
                     "
BASE COUNT         1957 a         3439 c         3281 g         2178 t
ORIGIN      
        1 gttcccagac ttgcgcgtcg ttgatctgtt taaagagctc gctcttcctg agaacaccga
       61 acttgtcgct gtccggaacc tcgcgctggt agacgctgag cttgccgacc tgcgcaatgt
      121 ccagcgccag atccgcccat gtgggatacc gatcctcggg ccgcttgctc aagaccctta
      181 atatgatcgg atccagtttt gcggatagag aagaccggag gacactcggg agcgcaggct
      241 cctcttccat gattttttgg aatagcgctg tcaggctcga ggcggtgaac ggccgctgcc
      301 ccgtgagcag ctcgtagaat acgacaccca ttgcgtacat gtcgctgtga tgcgtgagcg
      361 gcgccccact gatttgctcg ggggacatgt aactcggcgt cccgacgagc aagtcctggg
      421 tcgactgcgt cgacttgagc aaagccgctc caaagtcggc gaccttgatg ttggtgccct
      481 gtgtgaccag gatattcgcg ggcttgatgt cccggtgcac gatcccctgc cggaacgcat
      541 aatccagggc gctgcagaat ttgaacccga tctggatcac atcctccatc ggcaggaggc
      601 cctcgggctt gatgaacttg gcgaggctgc ccccgggcac atactccatc gccacatagc
      661 tggaatcctc ttccatcacc gcttcaagta tggacacgat atgggggtgt gcgagctttc
      721 cggcgagagt cgcttcgttc atgaattgcc cgcgggtgag ctcccccacc tccgaatcgc
      781 ggagaacttg actatcgatg accttgagcg cgacgtcctt gcccgcatag gtgtcgagcg
      841 ccaggtagac cttgccggag gcgcctgctc ccagctcttt cttcaactcg tatttcccga
      901 ctcgcgtctg cacctgtccc cctttacggc ctgaggaacg cgattctaac ttgaatcgct
      961 gacgtccccg gcaaggcccg aaccctggtt ttgaatgcgt ggctccccga tgggatctgc
     1021 aaacgcgaca atcaaaatgc aagctagctg ctgggcgacc cgacagctat cggcaccaat
     1081 tttagattac ccggtttggc aagacatatt cacgtctctc ttaagaatca gtgcgatggc
     1141 tctcgcaacg ttagcatcac gtgaaaaaac gtgcgcggct cgcgcaagcg gccgactgaa
     1201 gcacgagaaa gctcagttgc cgagtatgat gccggcggca agccccgtcg cgcgcgcgta
     1261 atccccgccg gagagtttct tgccgtcctc gccgcgcgcg cgcagcacct cgatcagccc
     1321 gccttgcgcg gtgaccttga agctcgtggc ggtgacctcg gagacctcgc caatcttgcc
     1381 cacgacgtcg gcaaaacggc ggaacaggtg cttgcgcgca tcgaagatct gcaacttctt
     1441 gccgttcagt gtagtccagg cacccggtgc ggggttgcag ccgcggatga tgttgtagac
     1501 gaaatcgacg tggtgcgccc aatgaatccg cgcctcggcc tcgcggcacc aaccctcgta
     1561 actcgccttg gactcgtcct gcgcgatttc cctgtgcttg cctgcgaata cgagatcggc
     1621 cgcctccagc atggccttca ctcccatcgg gaacagcttg ttgaaataga cgtcgccgat
     1681 cgtctcgtcg gggccgatgt cgcactccct ctgcagcacg atcgggcctt catcgagccc
     1741 ctcggtgggg cggaagatgg ttagcccggt gcgcgtgtcg ccgcgaatga tcggccagtt
     1801 gatggacgaa gggccccggt acttgggcag gagcgagggg tggtactgga cagtgccgag
     1861 gcgtggaatc ttgaccatgc tgtcgggcgc gaactgcagc acgtaggcca tgatgccgag
     1921 atccactttc aggcccgtca tcgcatcgtg cgcctcgggg ctgcgcagtg aagcgaactg
     1981 gaataccttg agcccctttt cctccgccgc cgtacggagg aggtcggcct tggcgccagc
     2041 cttttccggt gcgcagaaca cgcccgtgac ctcgtccttg cgggcgagaa acgcctccag
     2101 aacggccttg ccgaagtcct gctgtccaat gatggcgatt ttcatgactc ttctttcttg
     2161 gaaacaggtg aggtgtgagg agagaggggt ggggtgtgaa ccgcaaagca actcgccgcc
     2221 tcactcctca cccttcaccc ctcacgaaga aagcggcggc tacgctgctt tcttgggcgg
     2281 cgcggaaaac gcgccggcct gcttcaactg cgcgatcctc tcattgttgt agcccagcac
     2341 ctcgcgcagg atctcgtcag tgtgctcgcc gaggagcggc gagcgcttga tctccggtgg
     2401 ggagtcggac agcttgatcg gcatgccgac gttataccac ttgccgcgct gcgggtggtc
     2461 cagctcgacc cacatctggc gtccgcgcac atgctcgtcg tgggtgatgt cctcggtgcc
     2521 catgaccggc ccgcacggca cgtcgagctc gttgaggatc tgcataaact cgcgtttgtc
     2581 gtgtttcagg gcgaactcgc caatcagctc ccacatgtcg gcctggttcc tgcgccgctc
     2641 gtcgatggtg gcgaacttcg ggtcggtcac gaggcccggc aaaccgagtt cgacgctgat
     2701 ccgcctggcg agcgcatccc acacggcttc ctgcaccacg atgtagatga agtcgttcgt
     2761 gccgcccggc ttgcagcgga ccgcgttgcc gagctgcccg ccgcccgagt cgttgccgcc
     2821 gcgcggcacg tcgcccatgc ctttgtgcgt cggcaccgag tactcggaaa ggcctccgcg
     2881 cgtcaggcgt tggtggtcgc ggaacttcac gcggcacagg ttcatgatcc cgtccatcat
     2941 cgccacctcc acgtactggc ccttgccggt gcggtcgcgc tggcggagcg ctgcgagcag
     3001 cccgattgcc agatgcaggc cggtgcccgt gtcgccgatc tgcgcgccgg taacgaacgg
     3061 cgggccgtcg gggacgccgg tggtgctcat cgcgccgccc attgcctgcg cgacattctc
     3121 gtacgccttg aagttggcgt aaggaccgct cgagccgaag cccttgatcg agcccatcac
     3181 gatgcgcggg ttgatctcac ggattttctc ccaggggaag ccgaagcggt cgagcacgcc
     3241 cggcccgaag ttctccatca cgatgtcgca cttcttcagc agatcgacga acaccacctt
     3301 gccttcagcg gtcttcatgt tcaccgtgat cgagcgcttg ttgcagttga gcatggtgaa
     3361 gtacaggctg tcggcgttcg gcacgtcgcg cagctggccc cgcgtcgcgt cgccctgcgg
     3421 gttctcgaat ttgatcacat cggctcccat ccaggcgagc agctgcgagc aggtcggccc
     3481 cgcctgcacg tgggtcatgt cgagtatgcg aattcccttg agtgcttcca ttctgatctc
     3541 caaaacttct gcggcagatg aaaggctacc tgccctcgct caactgtttg acttatttct
     3601 tttttccgct cgtcggattc aggctcgtga tgcgaccgct ttcggtgccg gcggtttcat
     3661 cgatcacggc attgatgagc gtgggcttgc gcgagcggat ggcttcttcc attgccttgc
     3721 gcaactcggc cggggtcgtg gcgtgcacgc ccacgccacc gaaggcctct atcagcttgt
     3781 catagcgtgc acccttgacg aacacggtgg gtgccacatc ggggccgccg gtcggattga
     3841 cgtccgtgcc gcggtagacg ccgttgttgt tgaagatgac cacgcagacc ggcaggttgt
     3901 agcggcagat ggtctcgact tccatgccgg agaagccgaa ggcgctgtcg ccctcaatcg
     3961 cgatcaccgg ctggccgctg accacggcgg ccgcgactga aaagcccatg ccgatgccca
     4021 tcacgcccca agtgccgacg tccaggcgct tgcgcggctg gtacatgtcg acgatgctgc
     4081 gggtgaagtc gagcgcgttg gcgccttcgt tgaccaggat ggcctccggg ttggccttga
     4141 tgatgtcgcg caccacgttc agcgcgctgt gaaaattcat cggcctcgga tccttggcca
     4201 gggtctcggc catcttggcg aggttcttgt tcttgcgctc ggcgatagcg cgcagccagt
     4261 cagccggcgg cttggcccag ttggagccga tgccggcgag cagcgctgac acgcaggaac
     4321 cgatgtcgcc gaccagcggg gcgtggatac ggacgttgct gtccatttcc tgcggcgaga
     4381 tgtcgatctg gatgaacttc tggccgcccc agtccttgtg gtccttgccc ccccaggtct
     4441 tgcccttgcc gtgcgagagc agccagttca ggcgggcgcc aatcagcatc accacgtcag
     4501 cctcggccag cacgtaggag cgggcggcag cagcggactg ctcgtgcgtg tcgggcagca
     4561 ggcccttggc catcgacatg ggcaggtaag ggatgccggt cttctcgacc agggcgcgaa
     4621 tgtccgcgtc ggcttgggcg taggcagcgc ctttgccgag aatgatgagc ggacgcttgg
     4681 cgctcttgag cagttcaagg gcgcgctgca cggcgtcccg ggacgggatc tggcgcggcg
     4741 ccggatcgac caccttgatc agcgatttcc tgccggcttc cgcgtccagg gtctgagcga
     4801 acagcttggc cggcaggtcg aggtagacgc cgcccgggcg gccggagacg gcggcgcgaa
     4861 tggcgcgcgc gatgcccaca ccgatgtcct gggcgtgcag cacgcggaag gcggccttgc
     4921 acagcggctt ggcgatggcc aactggtcca tctcttcgta gtcaccctgc tggaggtcga
     4981 cgatctcgcg ctcgctggag ccgctgatga ggatcatcgg gaagcaattg gtggtggcgt
     5041 tggccagtgc ggtcaggccg ttgaggaagc cgggggcaga caccgtcagg cagatgccgg
     5101 gtttttgcgt catgaagccg gcgatggcgg cggcgttgcc ggcgttctgc tcatggcgga
     5161 acgaaatcac gcgcatgcct tcggcctgtg ccctgcgggt gaggtcggtg atcgggatac
     5221 cgggcagacc gaagatcgtg tcgatgccgt tgagtttgag ggtatcgatg accagttgaa
     5281 agccgtcggt cacttcctgt acaagctgca tgtcggcggc ggttgcagca accgggtttt
     5341 caacgtttcc catgatggtc tccttggcgt aaatggcagc ggctgtcagc taacatcttg
     5401 aaggggctcc aaatataggc tcctggcggg ggcgcagcct tgaccgcggt caagaaccag
     5461 cggttatact cgttccgttc cccccggcgg ccttaccgca ccgcagcgaa ttttccatgc
     5521 ctgtcctcgc ccagcatccg gaagcgaaga tcatccgcct gcagttgcgg caaaaccggg
     5581 ccctcaagca cctcgacgcc gcgcaatggc gcgagctcga tcccctgctg gaaatcgcgg
     5641 actatgcgaa gggcgagtcc ctcgagcatc agggggactg gtccatggaa cagtatttcg
     5701 tcatcgatgg gatcctcaag cgcgtggtgt cgaacgccca gggcaaggaa atgatcctgc
     5761 gtttcgccgc cgaagccgac atcgacacca gctacgccgc ctggcgcttg aagaccccca
     5821 taccctattc gatccgggcc gtcaccaagg tgcgcgcggc caagctctcg atgccgaact
     5881 gggtgcagtt cctcgagcgt caccccgcga tcaagagcgc cttcgaattc gaaatcatga
     5941 agctgatgag cgaagtcatg gcgcatacca tcaccctgca cctgctcgac gccccgggac
     6001 gggtgcagcg tttcatgcgc aaacacagcg aactcgcaga ccgcctgccg aagaaagagc
     6061 ttgcctgtta cctcaacctg tcgcctgaga cgctaagccg cctcaagcgg cggggcaaga
     6121 tctgaccgcc ggacacagcg cggccaggct gggtcgttca gacaggctaa aagtaaaacc
     6181 gcctcagcgt cggtttttgg aggcggtttt gccaaaggag tttatcggac cagggtccga
     6241 caaactccta ggatgatcaa gccgagggcc aggaaagacc cgacgggcag gatcgtgaca
     6301 ccttgccccc cagttaaata atgcaggcaa gcgtcagatc gccgctcagc ttctttgagg
     6361 gcagcggctg gtcgctcgct cgcgaaggca agacttcgct cagccgacgc ggggctaccc
     6421 ctgggcgaac ggaatcaccc ccgggatcag ccatttctcc cacatctcgt ccgtcttcgc
     6481 ctggaagtac gcgatatctt cctcggtaaa gtgggcaaag cgcccctgcc tcagcaagta
     6541 ctcgccaacc ggcttacgct tggccgcgcc gctcgcgatg tgcttgctct tgccgtacat
     6601 cttgagcttg cggtcttcga cctcgtagag gggccagatg ccggtttcga ccgcaagctc
     6661 acccagttca tgggagtact tcggatcgta gtcccagcct ttggggcaag gatcgagcga
     6721 gtggatgaat gtggggccgc caatgctcaa cgcccggcgc accttgttca acgtgtcgac
     6781 agcgtttccg gagcaaacgg tggcgacgta tttacagtcg ggatggcctg ccgccagcat
     6841 cgccgcggta ttctttttcc agcgcctgtg catgatgcga tgcaccttac ccggcgggct
     6901 gaaggtggtg ttggcaccgt acggcgatga acccgacacc tggatgtcgg tgttggcata
     6961 cgattcgttg tcatacagga agatcagcag gttgtactgc atgtgctgga gtgccgccga
     7021 aatggccgaa agccccatgt cggcgccacc gccgtcgccg caaaacgcga tgacgttgat
     7081 cggctccttg ctcatcttgc ccttgcgcat caaggcccgc agcgcggcgg cggtgccggt
     7141 ggctccggaa ccagccgatc cgagctgtgt gtgcatccac ggtaccaccc acggggtcga
     7201 gtaatacgtg gtgttggcca catacatgca gccggtggcg ccgagcacga tcgtgcgcgg
     7261 gccggccgcc ttgatcatga gtctcatgac cagcgccgac tcacacccct gacaggtgcg
     7321 atggcccgaa gtgtaatact cctcacgggc gaccttcttg atgcccttcc aggggtcgag
     7381 aacctgcggc gaagtgaaat cgcccacatc tgccttcagt ttcgtttctt ccatggtggc
     7441 tactccctca ctccgatcca gtaggtattt tgttcatcga tgtcggcgcc gcgcgcagcg
     7501 tcgaatacct tgctggtcac atcgatgacg tcattggtac ggacctcgcg accgcccaga
     7561 ccggcgatga aggagaggat cttgggccgg ttcttcacgg ggtagagagc agagcgcagg
     7621 tcgttggcca gcacgccgct gaagaaggtc gagccgaagc tgaagtcgcg gtcgattacg
     7681 cccactgcgc gcacccccgc gagcgcgtcc tgcagctcgc gggtggggaa cgggcggaac
     7741 cacctgactc gcacgaagcc gaccttcttg ccctgctccc gcatcttgcg cacggcgact
     7801 ttcaccggca tcgccagcgt gcccattccc accacggcga cctcggcgtc ctccatcatg
     7861 tagcgttcaa tgaagggctc gccgccgcgt ccgaacttgg cgttgaaatc gtcgtaggcc
     7921 tcgatgatga catcacgggc acggcgcgcc gccatgtcgc tctggcggcg catctccatc
     7981 agccagtcct cgttcacctg cggagcgatc gtgattggat tgtccggatg caactgctta
     8041 tctccccggt tataacgcgg aaggaagctg ttgaccatcg cctggctggg gactttgacg
     8101 atcgactgtg agtgggtgag gaacgcgcca tcacagctaa tcgcgcaggg aaggaagacg
     8161 cgcggatctt cggccacacg gtaggcgatc agcgccgtgt ccaacgcctc ctgcgcggtc
     8221 gacacccagc acagcatcca gccgaggtcc cgcaccgcga gcgcatcgtt gtgctcgact
     8281 ccgaaggcgc cgggatcgtc cagggcgcgg ttgccgacca tcgcgaccat cggcaggcgc
     8341 agcccggcgg taaccgccaa cgcctcgaag gcgtagaacc agccgacgcc gctcgatccc
     8401 acgaacacgc gggccccgac ggccgacgcg tgcttcacga tctcgaactg cgagtgctcg
     8461 ctctcggcaa cgatgaattc acagtcgagg tcgccgtcgg caatcatctt cgatacgtac
     8521 tgcatgacgg tgtcgtaggg acggatcggg tacgcggtga tcacgtccac atcggccagc
     8581 cggacagcat gcgctaccgc ttcgctgccg ctgatcaacg cctccgtctc agtgcgcggt
     8641 gcgaccttgc tgtccgcttt cttcgcagta gctgtgtcag ctgggctcat tggctatctc
     8701 ctcagcatac tggcctagat gatggaaccc gtgcctacgg gcgggctgat cggcaacctt
     8761 ttcggacaac cacgctgcat aggcttgctt gtccttgtgc cacatttccc actggctccg
     8821 gtgatccttg aaggcttcct cattgaccat ggtgatgcat ttgtccaccg ggcagacagc
     8881 ctcgcatatg ccacaaccgc agcaggcttc catgttggcg tcgtaatagc cgtcgggcgt
     8941 aatgtcgaag caggtatccg ggcattgcag ccagcaaatc gtgcacttgg tacaggtgtc
     9001 gaagttgatc accggccgca tcgtgcgcgt cgtgaacttc ttgaaggtgg gattgcgcgc
     9061 ggggacgtag ccaccgtccc ggcccggaac ctcctgcttg aggccgacgc cgcgaatcac
     9121 cagacactcc tccatcttct tccagcccgg caattcgaac ttgaagggga ccgcatcgtt
     9181 gccgttatca gggccaatcg cggtcgacgc gatcagatcg tgggccttcc tcgccgaagc
     9241 ctccttcgcg gcattcttct tcggctcgtt gatcgccgct acgatggagt ccaggctgat
     9301 gatgtgcggg gcgaccctgg ccagcgcccc gaggaggcgc acgtcggtgt gatcgtcctt
     9361 gtacacccac aaccccgaga agctggcgct cgccttgacg atagcgagat ggtagggctt
     9421 gcccttgcgg tggatgtcgc cgatcaggct ctccggcgag cgggtcgagg tgaccagaag
     9481 cgtgccgccc tccttgagca acttgttgat cggttgcaag ccataccagg cccaggactc
     9541 gatacccttg cagagggtgt cgtcagcgac tatggtgacg tcgttgttgt cgggctcgta
     9601 cctcgacagg tactcctcca tctcttcttc ggtatcagca acgacggcga actgcttggc
     9661 cgggatgccg ttgcgttcag gggagtcacc gtagcgcccg aaggcgatac cgatcttccc
     9721 ttcctttctt gcagcaaaga caatcccccg gcagatattc ttggctaaag ttttctgaaa
     9781 aatgccgcgg tagacgacct ccaccgaacc ggtgctcata gcacatccct cctcatggcc
     9841 tggacgcaga ggaaatcagc gtatcagttc gaaggtctct ttgtttgatc taggtcaaat
     9901 ccggtgacca cccactgtcc tccacgagcg gactcacaga aaaagaacgg gccgaacgcc
     9961 caacatacaa gacgccccga gagccgaggt ctgcaaacag ggggcagatc ggcgggcatg
    10021 cgctctactc ggagtggccg actacctcca gcatttcttc cacccgggtg aacttctccc
    10081 tcggcttgcc ggcagtcgcg ccgcgtcgca cctccgccgc atcgatcttt tgccagtccg
    10141 cgtagctcac cacgcgcacc gtgcggctct gcaacaaagc cgtcagtcca tcagcgccgg
    10201 ttttcgcgcc agcggaaaga ttcggcaagt ctgccagaat tgaatttacc gtggcgacac
    10261 tatcctcgcg attggtgcca atgatgcccg tcggaccacg cttgatccag cctgcgacat
    10321 ataagcccgg aacgacgttc tgcccatcga cgatgcgtcc atcccgactg gggaaaatgc
    10381 ctcgaccgtc gtcaaaagga acacccgcta tgggaacacc ctgatagccg atgctgcgaa
    10441 acagcacacc gcacgcaagc tcctctacat cacccgtttc acgtgcgacc tgctgcgaag
    10501 gttccccctc cagacgattt cgcatcagga ccacccgttc aagccttcct gtgcctttga
    10561 gttcctttgg actcttgagg aactggatgt ggcagcgccg gtgccgcgtg ggcggcggtc
    10621 gggaagcgaa cccttgcagc acttcatagc tcttgatatt ggcacggttg cgcctgtccg
    10681 caagctcctc gcggctggcc ggattcaagt caagatcgcg tggatccacg accgggtcac
    10741 agtccgccag gttcccaagc tctctcagct cctgatgagt gaacttcgct tgcgccggac
    10801 cccggcgtcc aatcatataa atgtcacgga ttctgctctc cgccaatacc tcgag
//
LOCUS       DBNGWZ010000244         3571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1913393, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000244 DBNGWZ010000000
VERSION     DBNGWZ010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1913393"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1049)
                     /locus_tag="AC16CX_07320"
     CDS             complement(198..1049)
                     /locus_tag="AC16CX_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012361.1"
                     /translation="MERTLIALGALLLCAGCMTAPGVPQPSVQSRIAEANARAAVLVR
                     AGDDAGAARQYEEALRLARSIEDADAIAAGAINLSIVYQWLGRDAAARGILSVVLDDK
                     SLAFPGRRRMQAELRRAILDLAARDAEGAAAWAQRARERCARLECDLAAAILNVQAQI
                     ALDAGRPEEGARLAQGAGEAARTRGDRAEAANALRTAGRARLALGDPAGAIQPLEEAL
                     ALDRELADPRKILVDLVELAQASLAKGDRDTARAYLERALVVGRAARDARSVEDIGAR
                     LRSLEGR"
     gene            complement(1049..2014)
                     /locus_tag="AC16CX_07325"
     CDS             complement(1049..2014)
                     /locus_tag="AC16CX_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR3012362.1"
                     /translation="MSETFKPPVRFPRLRRQVNLFFLLALLLIAGFVGLVAYKQGWLV
                     KHTTIHFVTMDALGISRGMPVKLYGLTVGSVKEMAIADEGVEVRLEINSEYMPRILKG
                     SRARFAREAGVVGASVIEIVPVRGSLAGLAPLGEGNRIEFDRSRGITEIVEEFRRQAA
                     PAFSEMRQMFSQINRSGDDAAAMLSALRGEAERLPETHTAVRKLIGDADRAAGQASAT
                     LDSTRRAAESLERETSVIAGKLSTALDSLNAAAGQVRETARQGEDVIRRVGPFLEQGE
                     TAAREGREVVDAAKRTWPLSGAFKEARDPTLPLDSFEARSSLPPR"
     gene            complement(2011..2640)
                     /locus_tag="AC16CX_07330"
     CDS             complement(2011..2640)
                     /locus_tag="AC16CX_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR3012363.1"
                     /translation="MTQAAPETPALALRYALKRDGRGEVAVAVPASCVRRVCLPDRET
                     KARFINAVLGARCERDEELELLGEDVTRLKAAGRSRLRRRVGVLTPTVGLISNLNVWE
                     NISLPAAYHGTPPLGQVAEAARQALAALGADPERLLARLPDELGAFDTKLVEFTRLLV
                     AAPELAVFDALGEGLDPGEHARVARFEAEYLARQPAGTLIYVDLPETRS"
     gene            complement(2637..3413)
                     /locus_tag="AC16CX_07335"
     CDS             complement(2637..3413)
                     /locus_tag="AC16CX_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012364.1"
                     /translation="MIRVLREFEGGVRLFFHVALRAGLLRRPAVLESFLRQVYFTGVS
                     AATGVVLRATAVGVLIIAFTIDVLDADTELAVKILLWVVMRELGPLAAALVVILRSGT
                     AVASELAMMRISGQTEALRHMGIDPYDYLVVPRVIGVMLATAALTLYIQFLAVLGGML
                     LSPFLINASLDELSNRFFELVTPIDLYYSVIKSLLFGFAIMNVCCYHGLNPPDLTHNA
                     VPKAVTRAVTQSAMVVLVINAVFAYLVFGVLFYGLITARV"
BASE COUNT          553 a         1284 c         1225 g          509 t
ORIGIN      
        1 cgcgatcggc gcgaccgtcg gcggcgtgct gggcagcggc cgctcctgaa ccgcaaccag
       61 catcaagaaa gggcggcctc ggccgccctt tttttacctc aaggcgaaag tcgaaaggcg
      121 gaaggaggaa agggaaaacc gctaccagcg tcttgccgcc tttcgccttt cgcctttcgc
      181 ctttcgcctt cgaacgctca gcgcccttcc agggagcgga gccgggcgcc gatgtcctcg
      241 acgctgcgcg catcccgcgc ggcgcggccg accacgagcg cccgctcgag gtacgcgcgc
      301 gccgtgtcgc ggtcgccttt cgcgaggctc gcctgcgcga gctcgacgag gtccacgagg
      361 atcttgcgcg ggtcggcgag ctcgcggtcg agcgcgagcg cctcctcgag cggctgaatg
      421 gcgcccgcgg gatcgccgag cgccaggcgc gcgcggccgg ccgtccgcag ggcgttggcg
      481 gcttccgcgc ggtccccgcg cgtgcgggcg gcctctcccg ccccctgcgc gagccgcgcg
      541 ccctcttccg ggcgcccggc atccagcgcg atctgcgcct gcacgttgag aatcgccgcg
      601 gcgagatcgc actccagccg cgcgcagcgc tcgcgcgcgc gctgcgccca cgccgccgcg
      661 ccctctgcgt cgcgcgccgc gaggtcgagg atggcgcgcc gcagttccgc ctgcatgcgc
      721 cgccggccgg ggaacgcaag gcttttatcg tccagcacga ccgacaggat tccgcgcgcc
      781 gccgcgtcgc ggccgagcca ttgatacacg atggagagat tgatcgcgcc agccgcaatc
      841 gcatccgcgt cttcgatcga gcgcgcgagc ctcagcgcct cctcgtactg gcgcgccgcg
      901 cctgcgtcgt cgccggcgcg caccaggacg gcggcgcgcg cgttcgcctc ggcgatgcgg
      961 gactggacgg aaggctgcgg cacgcccggc gccgtcatgc agccggcgca caggagcagc
     1021 gcccccagcg caatgagcgt gcgttccatc accggggagg cagcgagctg cgcgcctcga
     1081 agctgtcgag cggcagcgtc gggtcgcgcg cttccttgaa agcgccgctc aacggccacg
     1141 tgcgcttcgc cgcgtcgacc acctcgcgtc cctcgcgagc ggcggtctcg ccctgctcga
     1201 ggaacgggcc cacgcggcgt atgacgtcct ccccctgccg ggccgtttcg cggacctgtc
     1261 ccgcggcggc attgagggag tcgagcgcgg tcgagagctt gccggcgatg accgacgtct
     1321 cgcgctccag gctctcggcc gcgcggcgcg tggaatcgag cgtcgccgac gcctgccccg
     1381 cggcacggtc ggcgtccccg atcagcttgc gaacggcggt gtgcgtttcc ggcagccgct
     1441 ccgcctcgcc gcgcagcgcg gagagcattg ccgccgcgtc gtcgcccgag cggttgatct
     1501 gcgagaacat ctgcctcatc tcgctgaagg cgggcgccgc ctggcgcctg aattcctcga
     1561 cgatctcggt gatcccgcgg ctgcgatcga actcgatcct gttgccctcc ccgagcggcg
     1621 cgaggcccgc caggctgccg cggaccggga cgatttcgat cacgctcgcg ccgaccacgc
     1681 cggcctcgcg cgcgaagcgg gcgcgcgagc ccttcaggat gcggggcatg tattcgctgt
     1741 tgatctcgag ccggacctcg acgccctcgt cggctatcgc catctccttg accgagccga
     1801 ccgtcagccc gtagagcttg accggcatgc cccggctgat gccgagcgcg tccatggtca
     1861 cgaagtggat cgtcgtgtgc ttgacgagcc agccttgctt gtaggcgacc aacccgacga
     1921 accccgcgat cagcaggagg gccaggagaa agaacaggtt cacctgcctg cgcaggcgcg
     1981 ggaacctcac cggtggcttg aaggtttcgc tcatgagcgg gtctccggca gatcgacgta
     2041 tatcaacgtg ccggccggct gtcgggcaag gtattctgcc tcaaagcgcg ccacccgggc
     2101 atgctccccg gggtcgaggc cctcgccgag cgcgtcgaag accgccagct cgggggcggc
     2161 gacgagcagc cgcgtgaact cgaccagttt cgtgtcgaac gcgcccagct cgtcgggcag
     2221 ccgggcgagg agccgttccg ggtcggcgcc gagcgcggcg agggcctgcc gcgccgcttc
     2281 cgcgacctga ccgagcgggg gcgtgccgtg atacgcggcc gggagcgata tgttctccca
     2341 gacattgagg ttggaaatca ggccgaccgt gggcgtgagc accccgaccc gccgccgcag
     2401 gcgcgagcgc ccggctgcct tgaggcgcgt gacgtcctcg ccgagcagct cgagctcctc
     2461 gtcccgctcg cagcgcgcgc cgagcaccgc gttgatgaag cgcgctttcg tctcgcgatc
     2521 cggcaggcag acgcggcgca cgcaggaggc gggcacggcg accgccactt ccccgcgtcc
     2581 gtcgcgcttg agcgcgtagc ggagcgcgag cgcgggagtc tccggggcgg cctgcgtcat
     2641 acccgcgcgg tgatcaggcc gtagaacagg acgccgaaca cgagataggc gaagacagcg
     2701 ttgatgacca gcaccaccat cgcgctctgc gtaacagcgc gggtcaccgc tttcgggacc
     2761 gcgttgtgcg tcaggtcagg cgggttcagc ccgtggtagc agcacacgtt catgatggcg
     2821 aacccgaaca gcaggctctt gatcaccgaa tagtacaggt cgatcggcgt caccagctcg
     2881 aagaatcggt tcgacagctc gtcgagggac gcattgatca ggaacggact caacagcatt
     2941 ccgcccagca ccgccaggaa ctggatgtag agcgtcagcg ccgcggtcgc gagcatgacg
     3001 ccgatcaccc gcgggacgac caggtagtcg tacgggtcta tccccatgtg acgcagcgcc
     3061 tcggtctggc ccgagatgcg catcatcgcc agctcggagg cgaccgccgt gccgctgcgc
     3121 aggatcacga ccagcgcggc ggccagcggc ccgagctcgc gcatcaccac ccacagcaag
     3181 atcttcaccg cgagctcggt gtccgcgtcg agcacgtcga tcgtgaacgc gatgatcagg
     3241 acgcccacgg cggtggcgcg cagcaccacg ccggtcgcgg ccgagacgcc cgtgaagtac
     3301 acctgccgca ggaaggactc gagcaccgcc ggccggcgca gcaagcccgc gcgcaacgcc
     3361 acgtggaaga acagcctgac gccgccctcg aattcccgca gaacccttat cattggaagc
     3421 ggagtaacgc gcggatgacg aaaccaaggt cattttcttc cctgccggcc gattcggcaa
     3481 acaacgctgt ttttcccctc cgccggcccg cgccgtcagc ggggggtgag ggacctcccg
     3541 aaggatgaag gcagaaggat gaaggatgag g
//
LOCUS       DBNGWZ010000245         2417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1535948, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000245 DBNGWZ010000000
VERSION     DBNGWZ010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1535948"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC16CX_07340"
     CDS             <1..371
                     /locus_tag="AC16CX_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3012365.1"
                     /translation="QGMLVSKKLANTVISFENGQAVVAAFTKRLAEHQPVDVVILDVQ
                     MPVMDGITAARLIRSVEADHQKAKTPILFFSVQKCDEDLKRQIERFAPASYVNKGSDP
                     DPEQLADRIDQLVAHLQTRS"
     gene            494..>2417
                     /locus_tag="AC16CX_07345"
     CDS             494..>2417
                     /locus_tag="AC16CX_07345"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR3012366.1"
                     /translation="MSVDAIKALYAKMPAILAKARKKFGRPLTLAEKILVSHADDFDA
                     QVWERGKAQLLLRPDRVAMQDATAQMALLQFIQAGKKRVAVPSTVHCDHLIRAQSGAK
                     EDMARAIEENKEVYNFLRSASRKYGIGFWKPGAGIIHQVVLENYGFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGQLQGWASPKDVILYLCG
                     LLTVKGGTNKIIEYFGPGAATISATGKGTITNMGAELGATTSIFPFDEKMVAYLNITE
                     RADIAKLAQANRELLIADPEVFQSPEKFYDQIVEINLSELEPHVVGPHTPDLARPVSK
                     LPSEAKEKGYPMQLKAALIGSCTNSSYEDISRAAHIARQGLKAGLKAKTQFLVTPGSE
                     RIYHTMQRDGFMKTFEEMGGTVLANACGPCIGQWKREDIKPGEKNSIISSFNRNFPAR
                     NDGNAETLSFLSSPEVVTAMAFAGTLDFNPVSGTLTTKDGTKVHFEPPRGEELPAKGF
                     AKGEEGFEAPAENGDAVQVDLPPTSERLALLEPFPKWDGKDFAKLPILLKAKGKCTTY
                     HISPAGPWLKYRGHLDKISDNMFLGATNAFTNEAGKVTNALTGATGQTPSAVARAYKS
                     QGIGSIVIGDENYGEGS"
BASE COUNT          491 a          847 c          726 g          353 t
ORIGIN      
        1 tacagggaat gctggtcagc aagaagctgg cgaacaccgt catctcgttc gagaacggcc
       61 aggcggtcgt cgccgccttc accaaacggc tcgccgaaca ccagccggtc gacgtcgtga
      121 tcctggacgt gcagatgccg gtgatggacg gcatcaccgc cgcgcgactc atccgcagcg
      181 tcgaagccga ccatcagaag gccaagaccc ccatcctgtt cttctcggtc cagaagtgcg
      241 acgaggacct caagcgccag atcgagcgct tcgcgccggc cagctacgtc aacaagggct
      301 ccgaccccga cccggaacag ctcgccgatc gcatcgacca gctcgtcgcg cacctgcaaa
      361 cccggagctg agcgcgccgt cgatcgccgg ttgacaccgc cggcgctcct tccttacaat
      421 gcgtattccg tcgcggcgct cggttcccgg ccgcgtcgcc gctcaaccat cgtgatctcg
      481 gaggaggaat cgcatgtccg tcgatgccat caaagcgctc tacgccaaaa tgcccgcgat
      541 cttggccaag gcccgcaaga agttcggccg ccccctgacc ctcgctgaaa agatcctcgt
      601 ctcgcacgcc gatgatttcg acgcccaggt ctgggagcgc ggcaaggccc aactgctgct
      661 ccggcccgac cgcgtggcca tgcaggacgc cacggcgcag atggcgctct tgcagttcat
      721 ccaggccggc aaaaagcgcg tggccgtccc cagcacggtg cactgcgacc atttgatccg
      781 cgcccagtcc ggcgccaagg aagacatggc ccgcgcgatc gaggagaaca aggaggtcta
      841 caacttcctg cgctccgcct cgcgcaagta cgggatcggg ttctggaagc ccggcgcggg
      901 catcatccac caagtggtgc tcgagaatta cggctttccc ggcggcctga tcatcggcac
      961 cgactcgcac acgcccaacg gcggcggctt gggcatgctg gccatcggcg tgggcggcgc
     1021 cgatgcgggc gaggtcatgg cgggcctgcc gtgggaagtg ctgcacccca agctgatcgg
     1081 cgtgaagctc accgggcagc ttcagggttg ggcctcgccc aaggacgtga tcctgtatct
     1141 ctgcggcctg ctgaccgtga agggcgggac gaacaagatc atcgagtact tcggccccgg
     1201 cgccgcgacg atctccgcca ccggcaaggg caccatcacc aacatgggcg cggagctcgg
     1261 cgcaaccacc tcgatcttcc cgttcgacga gaagatggta gcgtacctga acatcaccga
     1321 gcgcgctgac atcgccaagc tggcccaggc caaccgcgag ctgctcatcg ccgaccccga
     1381 agtcttccaa tcgcccgaga aattctacga ccagatcgtg gagatcaacc tgtcggaatt
     1441 ggagccgcac gtcgtcggcc cccacacgcc ggacctcgcg cgccccgtct ccaagctgcc
     1501 gtccgaggcc aaggagaagg gctacccgat gcaactgaaa gccgccttga tcgggagctg
     1561 caccaattcg tcgtacgagg acatcagccg cgccgctcac atcgcccggc agggcttgaa
     1621 ggcaggcttg aaggccaaga cgcagttctt ggtcacgccc ggctccgagc gcatttacca
     1681 cacgatgcag cgcgacgggt tcatgaaaac gttcgaggag atgggcggga cggtcctcgc
     1741 gaatgcgtgt ggcccgtgca tcggccaatg gaagcgcgag gacatcaaac cgggcgagaa
     1801 gaattcgatc atcagctcgt tcaaccgcaa cttccccgcg cgcaacgacg gcaacgccga
     1861 aacgctctcg ttcctctcca gccccgaggt cgtgaccgcg atggccttcg cggggacatt
     1921 ggacttcaac ccggtctccg gcacgctgac caccaaggac ggcaccaagg tccacttcga
     1981 acccccgcgc ggcgaggaac tgccggccaa gggcttcgcc aagggcgaag agggcttcga
     2041 agccccggcc gagaacggcg acgcggtgca ggtcgacctg ccccccacca gcgaacgcct
     2101 cgccctgctc gaaccgttcc cgaaatggga cggcaaagat ttcgccaagc tgcccatctt
     2161 attgaaggcc aagggcaagt gcaccaccta ccacatctcc ccggccggcc cgtggctcaa
     2221 gtaccgcggc cacctggaca agatcagcga caacatgttc ctgggcgcga ccaacgcctt
     2281 caccaacgaa gccggcaagg tgaccaacgc gctgaccggc gcaaccggcc aaacgccgag
     2341 cgccgtcgcg cgagcctaca agtcccaggg catcggctcg atcgtgatcg gcgacgaaaa
     2401 ctacggcgag ggcagca
//
LOCUS       DBNGWZ010000246         3526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2669339, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000246 DBNGWZ010000000
VERSION     DBNGWZ010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2669339"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          723 a         1147 c         1031 g          625 t
ORIGIN      
        1 tatgacaatt acgaaccgcg ccgttcctgg tggatcgccg cgtatgttct gctcgccctg
       61 cttttgctcg gccgccagta ctacttgcaa agccggaaag attggaacgc gcgccgcgtc
      121 tcgatcagcg aagaggcctg gtctaatatc ttcggcggcc tctttacaac cgtgttcatc
      181 gcggtttggg tcgcatggct cattcctacc tcgctcgcca gtctgcaagc cgcgacggac
      241 acatggagca cgctcaccca acctattcgc gagcggctct ccaacgcggt cagcgcgctg
      301 gagtcgccgt acggcgcggg cggcatcaac ttctacggcg atacgctcgc gctgggacgc
      361 aatgccgcgt tgggcgattc gcccgtcttc accgtgcaag tactgggcgc gccggatctc
      421 gcccagcgtt actactggcg cgggcgcgtc tatgatttct acagcagcgg acaatggatc
      481 agcaccgccg cctcgcgcct ggacttcgag ccgcggaatg gcgatttaaa catcgccagc
      541 gcggagggca ggacgaaagc gctgttcgaa tttacatatc agtttccgac gcaaagcctg
      601 ctgtacgcgc cgtctcaacc ggtgtgggtg aatcacgccg cgtccgtgct ggtcacgccc
      661 gtcgacgcgc aaaccgacga tgtgatcgcg tgggaggcga ttccacccat ccaaaacggg
      721 aaccgctacc gggtccgcgc cgagatcgcc gacccgaaca ttcaagagct gcgcgccgcc
      781 ggcagcacct atccggaatg ggtcaaggat cgctacctcg aaatcccgga aaacatccga
      841 cccgaaattc aggcgctggc cgaaaagatc actgatggac aagaaacgcc atacgacaag
      901 gccgcggcca tcacggatta tttgcgtgca aaccttcaat acgcgaccga cctccccgtg
      961 ccgccgcagg gacgcgatcc gctcctgtgg gtgctgttca cttacaaaaa aggtttctgc
     1021 aattactacg cctcggcgga ggtcttgatg ctgcgctcca tcggaatccc ggcgcggctt
     1081 gcggtgggct ttgcgcaggg cgagcagcgg aatgacacat acaccgtccg taagcgcgac
     1141 gcgcatgcct ggcccgaggt gtatttcccc ggcgtcggct gggtcgagtt cgagccgacg
     1201 gtcagccaaa acgcgctggt gcgtccgcaa gccgcgccgc aggtcagcga tgcttctccc
     1261 gctcccgtcc ttccacaacg aaatccgatc cgcgatgacg aggcggccgc gccgccaact
     1321 cccggcgagt caaccaatgc cttacaaccc tttgctcaaa tcgtccccgg gcgcgcgctg
     1381 atcatcggcc tgtcgacgct tgggattgct ttgctgatct tcctgctcta tcgctatcgc
     1441 ctcctgacga atctgccgtt gtatttgtcg agggcgctcg aaggcagcga catcccaacg
     1501 cccgcctgga tcgagaactg gtcgaggtgg aatcaacttt cgccggtcga gcgatctttt
     1561 gcaagcgtca acctgagcct gcgctggctg ggcaggccgc agccgatgta cgccacaccc
     1621 gccgaacgcg ccgcgcttct aaagaagttg ctgcccaccg cttcggcgca catcgaagcg
     1681 ctgacctccg agcttgagtc ggcgctgttc acgccgcacc ccgcggattc gtcccgcgcc
     1741 cgacgcgcca gcctgctcat cgtgttacat accttgcgcg ccctcgtcct gaatttcctg
     1801 ggcgcaatcg aaggtcgtga tgtatactca aggtaattat ggatgcgaat aagacccgcc
     1861 ctaccaccgt ccacgaacaa atccatacgc tcggcctgca ggccggcaag ctgcgcgaca
     1921 ctttgaaaca gaagaagctg cgcgacctgc cgtcgaacgc gatggacgag ttggatcacc
     1981 tcgccggtac gctcgccaag gtcggccagc aaatggaggc cttcgaggcc cagcacggca
     2041 acctgctggc gctggccaac atcggccagg tgatcaattc gtcgctcgag ctggacgaag
     2101 tgctgcgcat cgtgatggac aacatcgtcc agttgacgca cgccgaacgc ggctacctga
     2161 tgctgcgcga cgagaagggc gagatggtga cgcgcgtggc gcgcaactgg gagcaggaat
     2221 ctatcaagtc cagcgaagcg gccaccagcc gcaccatcgt gcagcgcgtg atcgaaacgg
     2281 gcgagccgat cgtcaccacc aacgcgcagg aggaccagcg ctttgtgggc caggaaagca
     2341 tcgtggccct caacctgcgc tcgatcttgt gcgtgccgct caaggtcaag aacgatctga
     2401 tcggcgtgat ctacgccgac aatcgcatcc gcaccggcat cttcgccgaa agcgaacgtg
     2461 acctgctggt ggccttcgcc aatcaggccg cggtggcgat tgacaatgcc cggctgttct
     2521 cctcactccg tcaaacgctg gcggaggtga ccgaactcaa gaacctgatg gataacgtgt
     2581 tcgcctccat cgccagcggc gtgatcaccg ccaacgttca gaaccagatc acgctcgcca
     2641 atcgcgccgc gcaatccatt ctcggacaca ccgcgacgga catggtcggt catccgctgg
     2701 acgaggtgct ggcttccgtt tcgaaggaga ttcaaccgca cctgagcgaa gtccgcaaaa
     2761 ccgatagggc catcgtggac ctcgagatca gtcacaagat gccccagcgc ggcaacgtgg
     2821 actggcgttt gaatctctcg ccgctcaaag acgccagcca gaagactcag ggcatcgcca
     2881 tcgttttgga cgacatgact gagcgcaaga agctcgaagc gcagcgcaaa ctgctggagc
     2941 gcatggtctc gccggtggtg ctcgatcaga ttgacccgaa cagtctgcag atcggcggca
     3001 agaaagtgga catcacctgc ctgttcgccg acatccgcgg cttcactgct tacagcgaga
     3061 ggcatacgcc tgaagaactg gtggcagtgt tgaaccgcta cctggccgcg gccgccgaag
     3121 ccgtgctcgt ccacgagggc accgtggaca agttccttgg cgacgcggtc atggcctggt
     3181 acaacgcccc cgtgccgcag ctcgatcaca ccctgcgcgc ggtgaagagc gcgctggcga
     3241 ttcgcgatgc gatcaagaag ctacacaagg aacttcccgc cgaggctcat ctcgatttcg
     3301 gcgtgggcat tcattacggc gaggccgtgc tgggctggat cggcactgag aagcgcctgg
     3361 aatataccgc cctcggcgac agcgtcaaca ccaccaagag aatccaggag aactcgacca
     3421 agagtcagat cctgctcagc agggacgcct acgagcgcgt caagaagcag gtggacgccc
     3481 gcccctacgc cccgctgacg gtcaagggca agacccatcc gattga
//
LOCUS       DBNGWZ010000247         9869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2292924, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000247 DBNGWZ010000000
VERSION     DBNGWZ010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2292924"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="AC16CX_07360"
     CDS             complement(<1..268)
                     /locus_tag="AC16CX_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012367.1"
                     /translation="MKIAKTTAHTLRVPYHFPLIKETQHALVTFVEIETDDELKGHAF
                     SAYPLRFSISDFINREAREAIAGMDPLRPEAVRSVLYWKLSNKHY"
     gene            368..1465
                     /locus_tag="AC16CX_07365"
     CDS             368..1465
                     /locus_tag="AC16CX_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3012368.1"
                     /translation="MKITKVETIVVNMPMILSGSVVPKQGGRPRTSMDTLLVRVDTDE
                     GVTGWGEGFGHRIFPATRAALDTLIGPMCIGRDPTAISALADELQRGLAGVGRNGPAM
                     YALSAIDIALWDIAGKLANLPLYRLLGGSPRQTLPAYASLLRYGEPGAVTHHVEKALK
                     RGYHHIKLHEITLPPIQAAREAAGPDVPIMVDCNCPWTVAEAIAMARKLQPFNLKWLE
                     EPVWPPEDHAGLARVQSQGGIPTAAGENAMLPEFKSMLETGAITYAQPSVTKVGGVTQ
                     MRKVMALADAAGVAVVPHSAYFGPGLIASIHCIAAMPRESLVERYDADFAVNPLHDAI
                     LPDKDGCLAVPQGPGLGVEPDMAVVDKLRVG"
     gene            1508..2329
                     /locus_tag="AC16CX_07370"
     CDS             1508..2329
                     /locus_tag="AC16CX_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012369.1"
                     /translation="MQDDKAMRRVHFRLPTFAFRLCALALVGFIGAAYCAEFPTKPVR
                     MVIPIGPGSSMDITGRLVAQKMNESWNQPMIVDNRPGAGGNIGADVVAKAQPTGYTVL
                     FCSPSLAISRAAYRKLPYDALRDLEPVTQISSRGNVLVVHPSLPVNSVKELIALARSK
                     PGQLSFGSGGGSGSSDHLVGELFNLMAGVSITHVPYKSGPQAQNDLIGGRIQVYFGGI
                     PVNLPMIRAGKVKPLGVSLAKRSPALPDVPTVAEAGVPGYEVNVWYGLFAPRGTP"
     gene            2333..2515
                     /locus_tag="AC16CX_07375"
     CDS             2333..2515
                     /locus_tag="AC16CX_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012370.1"
                     /translation="MVAKISSDVARQLKSPEMQERLAALGVDAAGTTPAEFKALFTSE
                     IGKWAKVVKAAGVVLD"
     gene            2618..3124
                     /locus_tag="AC16CX_07380"
     CDS             2618..3124
                     /locus_tag="AC16CX_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012371.1"
                     /translation="MAAKTAKKAVAPAADRFDHTFKAIHDHEVPWQSDDVLGILKLPP
                     GVQVKVLNYDPVMKRIDMKHRFPPGYVEPAHTHKSWHSILVTKGRMCVAGKDLRPGDY
                     VFGWDIPHGPYEYPDGCEVFIVYMGDPTHNWEKAGLLKHQNVWKAETEEGKKGVVEHI
                     QKRKAGKV"
     gene            complement(3379..4128)
                     /locus_tag="AC16CX_07385"
     CDS             complement(3379..4128)
                     /locus_tag="AC16CX_07385"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR3012372.1"
                     /translation="MSKWNEGKVVTLRPWTDRLYSIQLEAAIEPFRAGQFGRLALPDE
                     SKGEMIARPYSFVNAPQEHPLEFHFITLDEGPLTKRLVQLKPGDPIWIAPKGSGFMTL
                     ADIQDARHLWLLSTGTALGPFLSICKTPDPWKRFEKVVLVHAVRRAAELSYHDRVREI
                     QAAHGQQFVFIPFVSREDTGFAIKGRVPQAIEDGRLEARAGIPLDAATSQVMICGNPD
                     MVEDTQKVLGARGLRKNRRKEPGHITVENYW"
     gene            complement(4198..4407)
                     /locus_tag="AC16CX_07390"
     CDS             complement(4198..4407)
                     /locus_tag="AC16CX_07390"
                     /inference="COORDINATES: protein motif:HMM:NF015424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3012373.1"
                     /translation="MKFGARNNVVGKVSEIKKGSLMCQVKVGVDHPIDITSVMTLDTL
                     ADLGVKNGDSVRVLIKAVNVILVRE"
     gene            4779..5087
                     /locus_tag="AC16CX_07395"
     CDS             4779..5087
                     /locus_tag="AC16CX_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3012374.1"
                     /translation="MGEFVKVAAKSEIPDQSAKLLKIGDKRLALFNLGGEFYAIDDTC
                     PHANGPLSEGSIEGEEVECPWHGSRFNIKTGEVTAEPAGENVCRYNVRATGDDIEVEV
                     "
     gene            complement(5199..5591)
                     /locus_tag="AC16CX_07400"
     CDS             complement(5199..5591)
                     /locus_tag="AC16CX_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012375.1"
                     /translation="MSTDETRAPAAPQIESISAVTLATHDMPRAVRFYSALGFSIRYG
                     GESAAFTSFHAGRGYLNLIAAPKDQRWSWWGRVIFYALDVEGMYRHAVAQGLQPQFAP
                     RDAEWGERYFHITDPAGHELSFAQPIVR"
     gene            complement(5677..6855)
                     /locus_tag="AC16CX_07405"
     CDS             complement(5677..6855)
                     /locus_tag="AC16CX_07405"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIR3012376.1"
                     /translation="MPAGITPGSSIQLMIAYRADHVGSLLRPPGLVSAREDAAAGRIS
                     QARFKAIEDRAVEDAIRLQERIGLPVITDGEQRRLSFQSRFAESVSGLGNWDLNTFLW
                     GHWRGDSSTVGDRTIERPAELGVVERLRRGRSLSVEDFVFLRARTTCTPKITLPSPSL
                     WANFWSAQSSRTAYPTLESFLADVVLILREEVGELVRNGATYIQLDAPHYALLLDPAT
                     RGFYESRGWSTERYLERGIEMDNAVMGDFPGVTFGFHLCRGNQASRWLASGGYERLAR
                     TVFRRVRAQRLLLEYDDARSGSFEPLKEVPEDKLVVLGLITTKRPHLEPIGVLEKRIR
                     EASRYVPLERLAISPQCGFSSSVIGNAITPADQEQKLRLVVETARRVWGEDAPLRSSS
                     "
     gene            7102..8070
                     /locus_tag="AC16CX_07410"
     CDS             7102..8070
                     /locus_tag="AC16CX_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012377.1"
                     /translation="MKTRQLLAVLGLSVSTLLPGAAIAQKYPAKPVRIVVGFAPGGAT
                     DISARAIAQRLTDRLGGSFVVDNRPGAAGNIAGDIVAKSVPDGHTILMANATIAIPSL
                     YAKLPFDVTKDLMALSLIALGPSVLAVHPSLPVKDVKGLIVLAKAGPDALAFGSGGVG
                     NSTHLAMELFMYMAGIKMIHVPYKGGAPSVVGLVTGEVQVLFTSIPSALTQINAGKMR
                     ALGVSISTRSKALPDVPTVAEAGLPDYYAASWYGLLVPAGVQKDIVNTLAKEVETIMR
                     EPDIQEKMLTQGFEPVGSNPEEFTKFIREEMPRWARIVRIAGIKPQ"
     gene            8075..9523
                     /locus_tag="AC16CX_07415"
     CDS             8075..9523
                     /locus_tag="AC16CX_07415"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3012378.1"
                     /translation="MTNRPRHHAALRRGKRGEPAIKKRTISERLAAEIVRAASRPPQD
                     PKLIHDVERVLLDSLGCALGAARSPSVNAARGWARRIAGYPSATILGTGEKSSVLGAA
                     MVNSTMVRDLDLNDTYFSRNPAHNSDNLGTCLAVGEAEGASAGEIINSILVAFEVQMR
                     ACEFTTKSFFKETGWDQTTLVTLASAAAAGVLLRLDQSRMGHALAIAGCYPAIGEMRV
                     GQLSMMKAASAGLAATRGIEAAYLAEAGMTGPREVFEGRRGMARLMLGECDWELLVAS
                     FGDWRLPRTCLKRYPAAYIIHSSIDLALDLRREHRLAAGDIDRVTVEAFGWLIEDMVN
                     GMGGASRYDIDSRETADHSLPFCVAVALVDGEYGLRQLDEKRWEAPEVREMMRKMRFI
                     HDREMDQRFPPDRPSRLTIVTRDGRTLVKEIPYPRGDFRSPFSDEEIADKFRSLASEV
                     LTPRQREIAIEQALDFSHGSVRALLDACTREQ"
     gene            complement(9614..>9869)
                     /locus_tag="AC16CX_07420"
     CDS             complement(9614..>9869)
                     /locus_tag="AC16CX_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3012379.1"
                     /translation="KTYVELGIGIAILTSISFDKYHDRGLRARDASHLFEPSTTYVRL
                     RSHTYLRKFVLDFIRMLAPHLSDEDIRSLLNRDASRAASR"
BASE COUNT         1927 a         2967 c         3189 g         1786 t
ORIGIN      
        1 tgtagtgctt gttggagagc ttccagtaga gcacggagcg caccgcctcg ggccgcagcg
       61 gatccatgcc ggcgatggct tcccgcgcct cgcggttgat gaaatcggaa atgctgaagc
      121 gcagcgggta ggccgagaac gcgtggccct tgagctcgtc atcggtctcg atctcgacga
      181 aagtcaccag cgcgtgctgc gtttccttga tcagcgggaa gtggtacgga acacgcagcg
      241 tgtgcgcggt ggtcttggcg atcttcatgg agcgtccttt acgtgatgag tgtcgagtgt
      301 cgggcgacca cactgcggac ttgcgcgagc gcgccagtcc agcggggccg tatacactat
      361 agccgaaatg aagatcacca aagtcgagac gatcgtcgtc aacatgccga tgattctctc
      421 cgggtcggtg gttccgaaac agggcggccg gccgcgcacc agcatggaca cgctgctcgt
      481 gcgcgtggac accgacgagg gcgtgaccgg ctggggcgag ggtttcgggc accgcatctt
      541 tcccgcgacc cgggcggcgc tcgatacgct gatcggcccg atgtgcatcg ggcgcgatcc
      601 caccgcgatc agcgcgctcg cggacgagct gcaacgcggc ctcgccggcg tcgggcgcaa
      661 cggcccggcg atgtacgcgc tgtccgcgat cgacatcgcg ctgtgggata tcgctggcaa
      721 gctcgccaat ctgccgctct atcgcttgct gggcggatcg ccgcgccaga cgctgcccgc
      781 ctacgcgagc ctgctgcgct acggcgagcc gggcgccgtc acgcaccacg tcgaaaaggc
      841 gctcaagcgc ggctaccatc acatcaagct gcacgagatc accctgccac ccatccaggc
      901 ggcgcgggag gcagccggcc cggacgttcc gatcatggtg gactgcaact gcccatggac
      961 agtggccgag gcgatcgcga tggcgcgcaa actccagccg ttcaacctca aatggctgga
     1021 agaaccggta tggccgccgg aggaccacgc agggcttgcg cgcgtgcagt cgcagggcgg
     1081 catcccgacc gcggcggggg agaacgcgat gctgcccgag ttcaagagca tgctggagac
     1141 cggagcgatc acctatgcgc agccgagcgt caccaaggtg ggcggcgtca cgcagatgcg
     1201 caaggtgatg gcgctcgccg atgcggccgg cgtcgccgtc gtgccgcact ccgcgtattt
     1261 cggaccgggc ctgattgcgt ccatccactg tatcgccgcg atgccgcgag agagcctggt
     1321 cgagcgctac gatgccgatt tcgcagtgaa cccgctgcac gacgcgatcc tgccggacaa
     1381 ggatggttgc ctggcggtgc cgcaggggcc gggacttggg gttgagccgg acatggcggt
     1441 ggtggacaag cttcgcgtcg gctgaagagc aaggcgaaag gcgaaaggcg aaaggcgaaa
     1501 ggcgaaagtg caagacgaca aagcaatgcg gcgggttcac ttccgccttc cgactttcgc
     1561 ctttcgactt tgcgcgctgg cgctcgtcgg attcatcggt gccgcgtact gcgccgagtt
     1621 cccgacgaag ccagtgcgca tggtgatccc gatcgggccg ggcagcagca tggacatcac
     1681 cgggcggctg gtcgcgcaga aaatgaacga gagctggaac cagccgatga tcgtggacaa
     1741 ccgtccgggt gcgggcggca acatcggcgc cgacgtggtc gcgaaggcgc agcccaccgg
     1801 ttataccgtg ctgttctgct ctccctcgct cgcgatctca cgcgccgctt accgcaagct
     1861 gccgtatgac gcgctgcggg atctcgagcc ggtcacccag atctcctcac gcggcaacgt
     1921 gctggtggtc cacccgtcgc ttcctgtcaa ctcggtgaag gaattgatcg cgctcgctag
     1981 gtcgaagccc gggcaactga gcttcggctc cggcggcggc agcggcagct cggaccacct
     2041 cgtgggggag ctgttcaacc tgatggccgg ggtcagcatc acgcacgtgc cctacaagag
     2101 cggaccccag gcgcagaacg acctcatcgg cggccggatc caggtctact tcgggggcat
     2161 accggtgaat ctgccgatga tcagggccgg caaggtgaag ccgctcgggg tgagcctcgc
     2221 caaacgctcg ccggcgctgc ccgacgtgcc aacggtcgcc gaggccggcg tgcccggcta
     2281 cgaggtcaac gtctggtacg gtctgttcgc gccgcgcggc acgccgtagc ccgtggtcgc
     2341 gaagatctcg tccgacgttg ctcggcagtt gaagtccccc gagatgcagg agcggctcgc
     2401 cgcgctcggg gtggatgccg cgggaacgac gccggccgag ttcaaggcgc tgttcacctc
     2461 ggaaatcggg aaatgggcga aagtggtgaa agcggcaggc gtggtgctgg attgacatag
     2521 ggcaccgcac cgtcgaattc agccagcttg tccgttgccg gcagtgccgt caaaataggc
     2581 gaagctcatc gttgaatctg acaggagggt ttgcgaaatg gcagcgaaga cagcgaaaaa
     2641 agccgtggca ccggccgcgg accgcttcga ccacacgttc aaggcgatcc acgaccacga
     2701 ggtcccgtgg cagagcgacg acgtgctcgg gatactgaag ctcccgcccg gtgtgcaggt
     2761 caaggtgctg aactacgatc cggtgatgaa gcggatcgac atgaagcatc gcttcccgcc
     2821 gggatacgtc gagccggcgc acacgcacaa gagctggcat tcgatcctgg tgaccaaggg
     2881 ccgcatgtgc gtggccggca aggatctgcg cccgggcgac tacgtgttcg gctgggacat
     2941 tccgcacggg ccctacgaat atcccgacgg ctgcgaggtg ttcatcgtct acatgggcga
     3001 tccaacgcac aactgggaaa aagccggcct cctcaagcat cagaacgtct ggaaggcgga
     3061 gaccgaggag ggcaagaagg gcgtcgtgga gcacatccag aaacgcaagg cgggcaaagt
     3121 ctgatgccgc gtgactcgtg actcgcggct cgtgactgag ctaaaaaaac cccgccgctg
     3181 cggggttttt gtcgccagca gtcggggaaa cgcaaccaga atcgatctca cagccgacgc
     3241 atgatcaagt cagctctgaa gcgagccatg aagggctgaa aacggcgcca aaagcggaca
     3301 ttcgccggat gccaaaccag atctggtgat gccatcgcga gtcgcactag aaggtaaagc
     3361 cctgccctga acgcttactc accagtagtt ttcgacggta atgtgccccg gctctttgcg
     3421 ccggtttttt ctcaaccccc gggctcccag cactttctgg gtatcctcca ccatgtccgg
     3481 gttgccgcaa atcatgactt gggaagtcgc cgcgtcgagc gggatgccgg ccctcgcttc
     3541 gagccggccg tcctcgatag cctggggcac gcgacccttg atggcaaagc cggtgtcctc
     3601 gcggcttacg aaaggaatga acacgaactg ttggccgtgg gcggcctgga tctcgcgcac
     3661 cctgtcgtgg taggagagct cggcggcgcg gcgcaccgcg tggaccagca ccactttctc
     3721 gaatcgcttc caagggtcag gagttttgca tatggacagg aacggcccca gcgcggtgcc
     3781 ggtggagagc agccacagat ggcgcgcgtc ctggatatcc gccaaggtca tgaacccgga
     3841 gcccttgggc gcgatccaga tgggatcgcc gggcttgagc tgcaccaggc gctttgtgag
     3901 ggggccttcg tccagggtga tgaagtggaa ctccagggga tgctcctgag gagcattcac
     3961 gaaagaatat ggccgggcga tcatctcgcc tttgctttcg tccggcagcg ccagacgtcc
     4021 gaactggccg gcccggaacg gctcgatggc tgcttccagc tgaatggaat acaggcggtc
     4081 ggtccagggc cgcagggtca ccaccttgcc ttcattccac ttgctcatgg cgatgccagc
     4141 taagaaaaag ccccggccct gagcacactg aacgacggca cagggggcga cccgggtcta
     4201 ctcgcggact aggatcacgt ttactgcctt gatgagcacg cgcactgaat ccccgttttt
     4261 cacgccaagg tccgcgagcg tgtccagcgt catgaccgag gtgatgtcga tcggatggtc
     4321 gacgccgacc ttgacctgac acatgaggga accctttttg atttccgaga cctttccgac
     4381 aacgttgttt cgtgctccga atttcatgcg gtcctccaaa gaagaaatgg gatcgaagtc
     4441 accggaatcg ggacagcgga taaagctaag ggatgatgat cccggactcg attcccgata
     4501 tgcatggtgc gcctccttgg cgatgctgtc attgccgatt caccttcgcg cttcaccccc
     4561 gccctcaagc catatccctg ttggagtgta gcccggcaga caggcatttt cgggggcaag
     4621 aaaccatggg tatttcgtgg tttttcgctg tggttgagct tctgacttct ttgactccac
     4681 ggatcacgga aggtaattgg caaaaccagc tgcactaagc ggtgcccgta ttgtccaatg
     4741 gcgcgaatag ctacagttcg cactaggagg agggcaccat gggcgaattt gtaaaagtgg
     4801 cggcaaaatc cgaaattccc gatcagtcgg cgaagcttct taaaatcggg gacaaacgcc
     4861 tcgctttatt taatcttggc ggagagttct acgccattga tgacacctgc ccccacgcaa
     4921 acggcccact gagcgagggc tccatcgagg gcgaagaagt cgaatgcccc tggcatggtt
     4981 cgcggttcaa tatcaagaca ggtgaagtaa ctgctgaacc tgctggcgaa aacgtgtgca
     5041 gatacaacgt tcgcgcgaca ggtgatgaca tagaggtcga agtctgagac ggtttgcctc
     5101 gaatcgagtc gatttggcgt actcgagtat ctcttctcac cggtccagaa ggtcgcgcac
     5161 gaggcaggaa gggagcagta agagggagag ccgatgcttt acctaactat cggctgagca
     5221 aagctcaatt cgtgtccggc cgggtcggta atgtggaaat agcgctcgcc ccattccgca
     5281 tcgcgcgggg caaactgagg ttgcaggcct tgcgccacgg cgtgccggta catgccctcc
     5341 acgtccaatg cgtaaaagat cacccgcccc caccacgacc agcgctgatc cttgggcgca
     5401 gcgataaggt tgagatagcc cctgcccgcg tggaaactgg tgaacgcagc cgactccccg
     5461 ccgtatcgaa tggaaaagcc tagcgccgag taaaaccgca ccgcccgcgg catgtcatgg
     5521 gtcgcaaggg tcactgcgct aatcgattcg atctgcggtg cggcaggggc tctggtctcg
     5581 tcggtactca tgttcgggga tccaattgag ggtttgatca tcggcgtcag gtgccggggg
     5641 gtgcgtggcg gcatgtggga gcgcatgtcg ggtgcctcag gatgaggaac gcaatggtgc
     5701 gtcctcgccc caaacccgcc gcgcagtctc cacgaccagc ctgagctttt gttcctggtc
     5761 tgccggggtg atggcgtttc cgatcaccga gctggaaaac ccgcattgcg ggctgatcgc
     5821 caggcgctcc aggggcacgt atcggctcgc ttcgcggatg cgcttttcga gcacgccgat
     5881 cggctcgaga tgaggacgct tggtggtaat gagcccaagc accaccaact tgtcttccgg
     5941 tacctccttg aggggctcga acgatcccga gcgcgcatcg tcatattcga gcaggagccg
     6001 ctgcgcccgg acgcgccgaa agacggtccg cgcgagccgc tcatacccgc cactcgcaag
     6061 ccagcggctg gcctggtttc cacgacacag gtgaaagccg aacgtaacgc cgggaaaatc
     6121 ccccatgaca gcgttgtcca tctcgatgcc tcgttccagg tatcgctccg tgctccagcc
     6181 tcgggattcg taaaagccgc gcgtcgccgg atcgagcagc agcgcatagt ggggcgcgtc
     6241 gagctggata taagtcgcgc cgttccgcac cagctccccc acttcctcgc gcaggatcag
     6301 tactacgtcc gcgagaaagc tctccaaggt gggataggcg gtgcgggaac tctgcgcaga
     6361 ccagaaattc gcccacaggc tcggactcgg caacgtgatc ttgggggtgc aggtcgtgcg
     6421 cgcgcgtaga aacacgaaat cctcgacgga caaagaacga ccgcgccgga gcctctccac
     6481 gaccccgagc tctgcagggc gctcgatcgt ccgatcgccg acggtagaag aatctccgcg
     6541 ccaatggccc cacaagaacg tatttagatc ccaattgccc aagccgctca cggactcggc
     6601 gaatcggctc tgaaaggaga gccgacgctg ttcgccatcg gtgatcacgg gcaggccaat
     6661 ccgctcctga agcctgatgg catcctcgac ggcgcggtct tcgatggcct tgaaccgcgc
     6721 ctgggagatt cggcccgcgg cggcgtcttc ccgcgccgaa acgaggccgg gggggcgaag
     6781 caagctgccg acatggtctg cgcggtaagc gatcatgagt tggatcgaac tacccggagt
     6841 gatcccagcc ggcaattaga ccgcgctctg aatgtctgct tcgggtcggt ttgggtcgtt
     6901 cgtggggatc caggatttgt tccgtcgctt tcggaggcgg cgtttctgcc cgatgcccgc
     6961 cgggtggcca attactctta tttgggcggg ttagtcacgc acagtatagc gccagcttat
     7021 acaagcataa cgtaatagtc ttgcgagtta tagcggcact gcgctagcat tttcgcgcac
     7081 aggcctagag gaggaaggat tatgaaaacg cgccaacttc tggcggtgct cggtctgagt
     7141 gtgtcgaccc tgctgccagg cgcggcgatt gctcaaaagt atcctgcaaa acccgtgcgc
     7201 attgtcgttg gctttgcgcc gggaggtgcc accgatatct cggcgcgcgc gatcgcgcag
     7261 aggctgaccg acaggctagg cggcagcttc gtcgtggaca accggcccgg cgcagcgggc
     7321 aatatcgcag gggacatcgt cgccaaatcc gtgcccgatg gtcacacgat cctcatggcg
     7381 aacgcgacga tcgccattcc gagcctttat gcgaagcttc cctttgacgt gaccaaggat
     7441 ctcatggcgc tcagcctgat cgcgctcggc ccttcggtgc tggctgtgca cccgagcctg
     7501 cccgttaagg atgtgaaagg actcattgtg ctggcgaaag ccgggccgga tgcgcttgcg
     7561 ttcggatccg gcggcgtggg gaactccacg cacctcgcca tggaactttt catgtacatg
     7621 gccgggatca agatgattca tgtcccatac aagggcggcg ccccgtccgt ggtgggcctc
     7681 gtgaccggcg aagtacaggt gctgtttact tcgatacctt ccgcgctcac ccagatcaac
     7741 gcgggcaaga tgcgagcgct tggcgtctcg atatccacgc gcagcaaggc gttgccggat
     7801 gtgcccactg ttgccgaagc cgggctgccg gattactacg cggcgtcgtg gtatgggttg
     7861 ctcgtgcccg ccggggtgca gaaagatatc gtcaatacgc tcgcgaagga agtcgagacc
     7921 atcatgcgag agccggacat ccaggagaaa atgttgacgc agggcttcga gccggtcggc
     7981 agcaaccccg aggagttcac gaagttcata cgtgaagaga tgccgcgatg ggcaagaatc
     8041 gtgaggatcg ccggcatcaa accgcaatga ggcaatgact aatcgcccgc ggcatcacgc
     8101 tgcgctccgg cgcggaaagc gcggtgagcc ggccataaag aagcggacga tcagcgagcg
     8161 gctggcggcc gagatcgtgc gcgctgcgtc gcggccgccg caagacccca agctcatcca
     8221 cgatgtcgag agagtgctgc tggacagcct gggctgcgcc ttaggggcgg cccgttcgcc
     8281 ttcggtgaat gcggcccgcg gctgggcgcg aagaatcgcg gggtatcctt cggccacgat
     8341 actggggacg ggcgagaaat cgtccgtgct cggcgcggcg atggtcaact ccaccatggt
     8401 gcgcgatctt gatctcaacg atacctactt ctcgcgcaat cccgcgcaca acagcgacaa
     8461 ccttggtacc tgtctggcag tgggcgaggc cgaaggcgcg agcgcgggcg agatcatcaa
     8521 ctcgatcctc gtcgcattcg aagtgcaaat gcgcgcatgc gagttcacca ccaagtcgtt
     8581 cttcaaggag acaggctggg accagacaac gttggtgacc cttgcatccg ctgcggctgc
     8641 aggagtgctg cttcgtctcg atcaatcacg catgggtcac gcgttggcga tagcgggttg
     8701 ttatccggcg atcggtgaga tgcgcgtggg ccagctctcg atgatgaaag ccgcgagcgc
     8761 cggcttggcg gccacgcggg gtatcgaggc ggcctatctc gccgaggcag gcatgacggg
     8821 gccccgcgaa gtgttcgagg gccgccgcgg catggccagg ctcatgctcg gcgagtgcga
     8881 ttgggagctg ctggtcgcgt cgttcggaga ctggcgcctg ccgcggacat gcctcaagcg
     8941 ttacccggcc gcgtacatca ttcacagctc gatcgatctt gcgctggatt tgcgacggga
     9001 acatcgcctg gccgccggcg atatcgacag ggtcaccgtg gaggctttcg gctggctcat
     9061 cgaggacatg gtgaacggca tgggtggagc gtcgcggtac gacatcgact ccagggagac
     9121 ggcggaccat agcctgccgt tttgcgttgc cgtagcgctc gtcgatggcg aatacgggtt
     9181 gcgccaactc gacgagaaga gatgggaagc ccccgaggtc cgggaaatga tgcgcaagat
     9241 gcgcttcatc catgatcgcg agatggatca gcggttcccg cccgacaggc cgtcgcgcct
     9301 gacgatcgtc acacgtgacg gcagaacgct cgtgaaggag atcccgtatc cgagaggtga
     9361 tttccggtcg ccattctccg acgaggaaat cgcggataaa ttcaggtctc ttgcttcgga
     9421 agtcctcact ccgcggcagc gagagattgc gatcgagcag gcgctcgact tcagtcatgg
     9481 aagcgtgcgc gctctgctgg acgcctgtac ccgggagcag taaccccgta gatcgtcatt
     9541 caggcgcggt caggaatccg tgctgcaggc gttcctccgg ataccagcct gcactggatc
     9601 ggcggccatc ccgctaccgg cttgcggccc tgctcgcgtc ccgattcagc aaggaacgaa
     9661 tatcctcatc gctcaagtgc ggcgccagca tgcggatgaa atcgagcacg aacttgcgca
     9721 gatacgtatg cgagcgcagt ctcacatatg tcgtgctcgg ctcgaaaaga tggctcgcat
     9781 cgcgggcgcg caggccgcga tcgtggtact tgtcgaagct gatcgaggtg aggatcgcga
     9841 tgccgattcc gagctccacg taggttttg
//
LOCUS       DBNGWZ010000248         2843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2669612, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000248 DBNGWZ010000000
VERSION     DBNGWZ010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2669612"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1001
                     /locus_tag="AC16CX_07425"
     CDS             30..1001
                     /locus_tag="AC16CX_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012380.1"
                     /translation="MKHAARLFLISVLSCAASGVAAADAAADYPSRPIRFIVPFVPGG
                     PSDVLSRLLGQKLTESWGQTVVIDNRGSAGGVVGFELGAKAPPDGYTLLLANGAGVTI
                     NPFVYLKLPYDPLRDFQPITQITSGAYFMVITPSLPARTVKEFIALAKAKPGGLNFAA
                     TGTNNLLASEQFNYMAGIKMVAVNYKGTGQAVGALLSGEVQMFIMSPLIAVPQVQAGK
                     LRAIGVTGLKRSPALPDVPAIAETLPGFENITWHSVLAPAKTPKPIVAKLSRELMRII
                     RQPDVHERFSSVGLDAVGSTPEELAALIKKEVVMYAKLVKQIGLKPQ"
     gene            1157..2137
                     /locus_tag="AC16CX_07430"
     CDS             1157..2137
                     /locus_tag="AC16CX_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012381.1"
                     /translation="MKAGKSSPVVILLVAIAALALPAPVTAQAYPAKPVRVVVPFAAG
                     GGTDIQARVIFKELQEQFKQIFIIDNRPGASGLIGADLVVSSPADGYSLLFTTATIAI
                     NATLYAGRLKFDPAKDLAPVILVSTTPLVLVVHPSVPAKSVKELVAIAKSKGAVLNAA
                     INVPGSTSHLSAEMFRQMTGAQLTPVPYKGGGLSMVALMSGEVDFQFAEGLLAAPQMR
                     AGKIRALAVSTAKPSAAFPDLPTVNSTLPGLVADNWFAMYFRAGTPRDIVVTINRGIG
                     KTLESKPVRALFEQDALTAIGGTPEDLGAHLKSEIVRYGEVIRKGKITAQ"
     gene            complement(2553..>2843)
                     /locus_tag="AC16CX_07435"
     CDS             complement(2553..>2843)
                     /locus_tag="AC16CX_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012382.1"
                     /translation="ATLHFPHYRLSGGRMKVWERPDSYLRDFYARAGADWHHSAWDFR
                     NVIAAGPDKVHLDVQFTRYRADNSVIGSYRSLWIVSKLNGRWAAQCRSSFAA"
BASE COUNT          566 a          862 c          870 g          545 t
ORIGIN      
        1 catttatttc ccaattcgag gaggactcaa tgaaacatgc agctcgtctg ttcctgattt
       61 cggttttgtc atgcgccgca tcgggcgtcg ccgcagcgga cgccgcggca gactatccca
      121 gcaggccgat ccggttcatc gtgccgttcg tgcccggtgg ccccagcgac gtgctctcgc
      181 gactgctcgg ccagaagctg accgaaagct ggggccagac cgtagtcatc gacaatcgcg
      241 gaagcgccgg cggcgtcgtc ggtttcgagc ttggcgccaa ggcgccgccg gacggataca
      301 cgctgctgct cgccaacggc gccggggtca cgatcaaccc gttcgtgtat ctcaagctgc
      361 cgtacgatcc cctgcgcgac tttcagccca tcacgcagat cacttccggc gcgtacttca
      421 tggtgatcac gccgtcgctg ccggccagga ccgtgaagga attcatcgca ctcgccaagg
      481 caaaacctgg gggcctcaac ttcgcggcga ccgggaccaa taacctgctt gcgtcggagc
      541 agttcaacta catggccggc atcaagatgg tggccgtgaa ttacaaaggg accgggcaag
      601 cggtaggtgc gctcctgagc ggggaagtgc agatgttcat catgagcccg ctgatcgccg
      661 taccgcaggt ccaggcggga aagttgcggg cgattggcgt gacgggactg aagcgcagcc
      721 ctgcccttcc cgatgtgcca gctatcgcgg aaaccttacc gggcttcgag aatatcacct
      781 ggcacagcgt gctggctcct gcgaagacgc ccaagccgat tgtcgcgaag ctctccaggg
      841 aacttatgag gatcatcagg cagccggatg ttcacgagcg cttcagttcg gtgggcctcg
      901 atgccgtggg ctcgacaccg gaggaactcg ccgccttgat caagaaagaa gtcgtgatgt
      961 acgccaagct ggtcaagcag atcgggctca agccgcaata gcgcgaaccg aaaggcgtgg
     1021 ttcgtgattc gattctgcag gaaggatcca aaggagccag ggtcgaattt caggtaattc
     1081 gagcctggct cctttgattc cgccgcaaaa ttcttacact gaccccattt acatttacat
     1141 caaaggggag gcgagcatga aagccgggaa atcttcgccc gtagtcattc tgcttgtagc
     1201 gattgcggcc ctcgcgctac cggcgcccgt aacagctcag gcgtatcccg cgaagccggt
     1261 gcgcgtggtg gtgcccttcg ccgccggcgg cggcacggat atccaggcac gcgtgatctt
     1321 caaggaactc caggagcagt tcaagcagat cttcatcatc gacaaccgcc ccggggcgag
     1381 cggtctcatc ggggcggacc tcgtggtcag ctccccggcc gacggctact cgttgctttt
     1441 caccaccgcc acgatcgcga tcaatgccac gctctacgcg ggccggctga agttcgatcc
     1501 cgcgaaagat cttgcgcccg tgatcctcgt cagcaccacg ccgctggtgc tggtcgtgca
     1561 tcccagcgtg ccggcaaaat cggtgaagga gctggtcgcc attgcgaaaa gtaagggcgc
     1621 ggtgctcaac gcagccatca acgttcccgg cagcaccagc cacttgtcgg cggaaatgtt
     1681 caggcagatg actggagccc agttgacacc cgttccctac aagggcggcg ggctttcgat
     1741 ggtggcgctg atgagcggag aggtggattt ccagttcgcc gaagggttgc tggcggcgcc
     1801 ccagatgagg gcgggcaaga tacgcgcgct cgcggtgtcg actgccaagc cgtccgcagc
     1861 ttttccggac ctgcccacgg tgaattcgac gctaccgggt ctcgtggccg acaattggtt
     1921 tgccatgtat ttccgggcgg gcacgccgcg cgacatcgtc gtgacgatca atcgcgggat
     1981 cggaaagacc ctggaatcga agcccgtgcg cgcgctgttc gagcaggacg cgctgaccgc
     2041 tatcggcgga acgcctgaag atctcggcgc ccacctgaag agcgagatcg tccgctacgg
     2101 ggaagtcatc cggaagggca agatcaccgc tcagtagccg gctctcctgc ggcgtgatag
     2161 gtgatgggtc tccccaccac ccttagtgga cagaccacta agaaaattgt tgatgtagat
     2221 cagcccttac caatccttag tggtctgtcc acgattttgt gctacatcga gccgcctaat
     2281 tgaacggtcg gcgcaggcgg cgccactgag acgccgcgaa tgccgttctt ctcgatcgac
     2341 cgggcgacga attccgaagg cgagtaaata agggacagac cacgtttccc gcatgtcaaa
     2401 aaggtggtct gtcccctatt tattgattta ttgacctgac ggcaacgcct caagtatgaa
     2461 ggtagaagga tgaaggatga gcaaaaaact gtagagcagc gcccttctgc cttctgcctt
     2521 ctgccttctg ccttctgcct tctgcgttcc gtctacgccg cgaagctgga gcgacactgg
     2581 gccgcccagc ggccgtttag cttcgagacg atccagagcg agcggtagct gccgatcact
     2641 gagttgtcgg cgcggtaacg cgtgaactgg acgtcgaggt gaaccttgtc gggcccggcg
     2701 gcgatcacgt tgcggaaatc ccacgcgctg tggtgccagt ctgcgcccgc gcgcgcatag
     2761 aaatccctca agtagctgtc cggccgctcc cagaccttca tgcggccgcc cgaaaggcgg
     2821 tagtgaggaa agtgcagcgt cgc
//
LOCUS       DBNGWZ010000249         4804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3299656, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000249 DBNGWZ010000000
VERSION     DBNGWZ010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3299656"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="AC16CX_07440"
     CDS             complement(<1..1169)
                     /locus_tag="AC16CX_07440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HIR3012383.1"
                     /translation="MYVSSLPQNAEFDYIIVGAGSSGCVMADRLSSGGRHRVLLIEAG
                     GRDNKLNIRIPLMVAKLLMDERVTWPFTTEPQTHLNGKPQLWVRGRVIGGSNSINGLL
                     FVRGDPKEYDKWRDAGCAGWGYFDMLPYFKRIEDYPEGDPAVRGRGGPIGVTNLGHFD
                     ELVDAFVGACEQNGFKRLADYNDGSYEGTFPLQYSTKNGMRASTAYAYLRPASKQPNL
                     TILTNAVATRVLTEGKRATGVEVSYGGGLQQVRARREVVLSAGPLKSPQILELSGIGK
                     ADVLQKFGINVVHHLPGVGENLRDHPNVRLTFECAKPITINDVLRSPLLQLKEGLRFI
                     FRREGLLTICSATAQTNFRSSKEVKQPDLVLRLLPVSGKDRYARTKAYGLDPFPG"
     gene            complement(1183..1374)
                     /locus_tag="AC16CX_07445"
     CDS             complement(1183..1374)
                     /locus_tag="AC16CX_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012384.1"
                     /translation="MSSRKERAERSADRWKFYQDDASKWRWKYIMQGKTAAQAYGSFD
                     RYSDCVQDARLHGYRDNSK"
     gene            complement(1378..2346)
                     /locus_tag="AC16CX_07450"
     CDS             complement(1378..2346)
                     /locus_tag="AC16CX_07450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012385.1"
                     /translation="MLRLAAILLFSWGCALYGAAPDYPTKPLRLVVVYPPGGGIDLLA
                     RVMGPQFTASWGQPIVVDNRPGAGTTIGAAVAAKASPDGYTLLMTDVSYAITPSLYAN
                     LPYDPRKSFAPISLVNLVTDVLVVHPSLPATTVKELVAHVKAHPGKVLYASAGNGTLN
                     HLAPEMLKAMAGIDLVHVPFKGAIAALTDVVAGRQQLYIGALASTVPHIRSGRVRAVA
                     ITGKKRSPMLPDLPTVAESGLRELRDYDVSAWYGLLAPAGTPRPIIQSIHQQVKLALQ
                     ARDVQQQLAEQGSEPVGSTPGEFEAFIAVEMKKWGAAVKTAGAKVD"
     gene            complement(2375..3880)
                     /gene="tcuA"
                     /locus_tag="AC16CX_07455"
     CDS             complement(2375..3880)
                     /gene="tcuA"
                     /locus_tag="AC16CX_07455"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3012386.1"
                     /translation="MPLRSQSYDVVVVGAGNAALCAALSAHENGARILVLERAPLAER
                     GGNSVYTDGKMRVVYDGIDDILALSPDLSPEEIQRSDFGRYTEDDFFDDMARITQNRA
                     DPDLCEILVRQSKATLRWMKTNGVRFHPNYGRQAYRVDGRFKFWGGAPIVVSGGGQGL
                     VETLVKSCERKGIEIVYGAWVQDLVHSDRGVSGVVAKINGETLTIHGRAVILACGGFE
                     ANAAWRSRYLGPGWDLAKVRGTRYNTGDGLAMALRIGAQPYGHWSGCHATGWERYASD
                     FGDLIQTPNFQRHSYTFGIMVNAEGKRFLDEGADFRSYTYAKYGHIVLEQPGQYAWQI
                     YDAKVQHLLLDEYRTRQVTKVTANTLEELADKMEDVDKEQFLKTVREFNAAVRTDVPF
                     DPNVKDGRSAPGLDVPRSNWANTIDTPPFEAYAITCGITFTFGGVRINTHGQVLDTNH
                     EVIPGLFAAGEMVGGIFYFNYPGASGLTSGAVFGRLAGASAGDYAKQKRAA"
     gene            3908..>4804
                     /locus_tag="AC16CX_07460"
     CDS             3908..>4804
                     /locus_tag="AC16CX_07460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3012387.1"
                     /translation="MQFISSHHSLPEYCVILLFRSVIRGTPMNLKQLRYICEVYRHGN
                     HISAAAEALHTSQPGISKQIQMIESELGFEIFLRRRNRIVGLTEPGQEAVAVAQRVLA
                     EVSNLKNLAADFNAIGGGHLTIATTHTMARYVLPKIIDRFMKKYPAIRLGLRQGNPEQ
                     VCELLDAGEADMAIVTDTMRPFPTLAAIPCFPLSRSVVAKAGHPILKARNLTLQEIAK
                     YPIITYDPAYSGRWKVMDAFKRAKIEPNIIFGAVDADVSKTYVELGIGIAILTSISFD
                     KYHDRGLRARDASHLFEPSTTYV"
BASE COUNT          850 a         1561 c         1492 g          901 t
ORIGIN      
        1 aagccgggaa acgggtcgag gccgtacgcc ttggtgcggg cgtagcggtc ctttccggac
       61 accggcagca ggcgcagcac gaggtcgggc tgcttgacct ccttcgagct gcgaaagttg
      121 gtctgcgccg tcgccgagca gatcgtgagc agaccctcgc gccggaagat gaagcgcaga
      181 ccctccttca gctggagcag cgggctccgc agcacgtcgt tgatcgtgat cggctttgcg
      241 cactcgaacg tgagccgcac gtttggatga tcgcgcaggt tctcgccgac gcccggcagg
      301 tgatgcacca cgttgatgcc gaacttctgc aatacgtccg ctttcccgat accggaaagc
      361 tcgaggatct gaggcgactt gagcggaccc gccgacagca cgacttcgcg ccgcgcccgg
      421 acctgctgca gtccgccgcc ataggagact tcgacgccgg ttgcccgctt gccttcggtc
      481 aggacgcgcg tggccaccgc gttggtcagg attgtgagat tcggctgctt gctggcggga
      541 cgcagatagg cgtatgccgt gctcgctcgc atcccgttct tcgtcgaata ctgcagggga
      601 aaggtgcctt catagctgcc gtcgttgtag tcggcgaggc gcttgaaacc gttctgctcg
      661 cacgctccga cgaatgcatc gacgagctcg tcgaagtgcc cgaggttggt cacgccgatg
      721 ggcccgccgc ggccgcgcac cgccggatcg ccttccgggt aatcctcgat ccgcttgaaa
      781 tacggcagca tgtcgaagta accccagccg gcacatcccg cgtcacgcca cttgtcgtat
      841 tccttcggat cgccgcgcac gaacaaaagc ccgttgatcg agttcgagcc gccgatcacg
      901 cggccgcgca cccaaagctg cggcttgccg ttcaaatgcg tctgcggctc ggtcgtgaaa
      961 ggccaggtga cgcgctcgtc catgagcagc ttcgcgacca tcagcggaat gcggatgttc
     1021 aatttgttgt cgcggccgcc cgcttcgatg agcaggaccc ggtggcggcc accggaggag
     1081 agccggtcgg ccatcacgca acccgaggaa ccggcgccga cgatgatgta gtcgaactcc
     1141 gcgttttgtg gaagcgacga gacgtacatt cgcgacctcc tcctacttcg agttatcacg
     1201 gtatccatgc aaccgggcgt cctgcacgca gtcgctgtag cggtcgaagc tgccataggc
     1261 ctgcgccgcg gtcttgccct gcatgatgta cttccagcgc cacttgctgg catcgtcctg
     1321 gtagaacttc cacctgtcgg cggagcgttc cgctctttct ttcctggagg acatgggtca
     1381 gtccaccttc gctcccgccg tcttcaccgc ggcgccccac ttcttcatct cgaccgcgat
     1441 gaacgcctcg aattcgcccg gcgtgctgcc gaccggctcg ctgccctgct cggcgagctg
     1501 ctgctgcacg tcgcgcgcct gcagcgccag tttcacctgc tgatggatgc tttgaatgat
     1561 cggccggggc gtgcccgcgg gcgcgagcaa tccgtaccag gcgctcacgt cgtagtcgcg
     1621 cagctcccgc agaccggact cggcgacggt gggcaggtca ggcagcatgg gcgaacgctt
     1681 cttgccggta atcgcgacgg cgcgcacgcg tcccgacctg atatgcggca cggtcgaggc
     1741 gagcgcgcct atgtagagct gctgccgccc ggccacgacg tcggtcaacg cggcgatcgc
     1801 gcctttgaac ggcacgtgca cgagatcgat gcccgccatc gctttcaaca tttccggggc
     1861 gagatggttc aacgtgccat tgccggcgga cgcgtataga accttcccgg gatgggcctt
     1921 cacatgcgcg accagctcct tcaccgtggt cgcgggcagc gacgggtgca cgacgagcac
     1981 gtcggtcacc aggttcacga gcgaaatcgg agcgaagctt ttccgcgggt cgtatggcag
     2041 gttcgcgtac aagctcggcg tgatggcata gctcacgtcg gtcatgagca gcgtgtagcc
     2101 atcgggcgag gccttggcgg cgaccgcagc accgatggtc gttccggcgc cgggccgatt
     2161 gtctacgacg atcggctgtc cccagctcgc ggtgaattgc gggcccatga cgcgcgcgag
     2221 cagatcgatg ccgccgcccg gtggatacac cacgacgagc cgcaatggct tcgtcggata
     2281 gtcgggcgcc gccccgtaca gcgcgcaacc ccaggagaac aggaggatcg cagccagtct
     2341 caacatagcg gacccggtgt gccggcgttt ggatctatgc cgctcgtttc tgcttcgcgt
     2401 agtcgcctgc gctcgcgccc gcgagacggc cgaacaccgc cccgctcgtg agcccgctcg
     2461 cgcccggata gttgaaatag aaaataccgc cgaccatctc gcccgcggcg aaaaggccgg
     2521 gaatcacttc gtggttggtg tcaagcacct ggccgtgagt gttgatgcgc acgccgccga
     2581 aggtgaacgt gatgccgcat gtaatcgcgt aggcttcgaa cggcggcgtg tcgatcgtgt
     2641 tggcccagtt cgagcgcggc acgtcgagtc ccggcgccga gcggccgtcc ttcacgttcg
     2701 gatcgaacgg cacgtcggtc cggacggcgg cattgaactc acgcaccgtc ttcaggaact
     2761 gctccttgtc gacgtcttcc atcttgtcgg cgagctcttc gagcgtgttc gccgtgacct
     2821 tggtgacctg gcgggtgcgg tattcatcca ggagcaggtg ctgcaccttg gcgtcgtaaa
     2881 tctgccaggc atactgtcca ggctgttcga gaacgatgtg gccgtacttg gcgtaggtgt
     2941 agctgcggaa gtccgcccct tcgtcgagga agcgcttccc ctccgcgttc accatgatgc
     3001 cgaacgtgta gctgtggcgc tggaaattgg gcgtctggat gagatcgccg aaatcggacg
     3061 cgtaacgttc ccagcccgtc gcgtggcatc ccgaccagtg gccgtaaggc tgcgcgccga
     3121 tgcgcaatgc catcgccagc ccgtcgccgg tgttgtaccg ggtgccccgc actttggcga
     3181 gatcccagcc cgggccgaga taacggctgc gccacgcagc gttcgcttca aagccgccgc
     3241 aagcgaggat caccgcccgg ccatggatcg tgagtgtctc cccattgatc ttcgcgacga
     3301 cgccggaaac gccgcggtcg gagtggacga ggtcctgcac ccaggcgccg tagacgatct
     3361 cgatgccttt cctttcgcag gacttaacca gcgtctcgac gagaccctgt ccgccgcccg
     3421 aaaccacgat cggtgcgccg ccccagaact tgaatctccc gtcgacgcgg taggcctggc
     3481 ggccgtaatt cggatggaag cgcacgccgt tcgtcttcat ccaccgcaac gtggccttgc
     3541 tctgcctgac gagaatctcg cacagatcag gatccgcgcg attctgcgtg atccgcgcca
     3601 tgtcatcgaa aaaatcgtct tcggtgtaac gcccgaagtc gctgcgctgg atttcctcag
     3661 ggctcaggtc ggggctcaac gcgagtatgt cgtcgatccc gtcgtagacg acgcgcatct
     3721 tgccatcggt atacacgctg tttccgccgc gctcggcgag cggcgcacgc tcgagcacca
     3781 ggattctggc tccgttctcg tgggcagaga gcgcggcgca cagcgccgca ttgcccgcgc
     3841 cgacgacgac gacgtcgtag ctctgtgatc gcagtggcat gaattcgccc tggctggtcc
     3901 ggaacgcttg caatttatca gcagccatca tagcctgcca gaatattgtg ttatcctgct
     3961 attccgttcc gttatacggg gaacaccgat gaatttgaag cagttgcgct acatctgcga
     4021 ggtgtaccgg cacggcaacc atatctcggc ggcggccgag gcgctccaca cctcgcagcc
     4081 cggcatcagc aaacagatac agatgatcga gtcggagctt ggcttcgaaa tttttttgcg
     4141 ccgccgcaat cgcatcgtcg gcctgacgga gccggggcag gaagcggtcg ccgtcgctca
     4201 acgcgtgctg gccgaagtga gcaatctcaa gaacctggcc gcggacttca acgccatcgg
     4261 gggcgggcac ctcaccatcg caacgacgca caccatggcg cgctatgtgt tgcccaagat
     4321 catcgacagg ttcatgaaga aataccccgc gatccgcctc ggcctgcggc aaggcaaccc
     4381 cgagcaggtg tgcgagctgc tcgacgcggg cgaagccgac atggcgattg tcaccgacac
     4441 gatgcgcccg tttcccacgc ttgccgccat tccgtgcttt ccgctgtccc ggagcgttgt
     4501 ggccaaagcc gggcatccga ttctgaaagc gagaaacctg acgctgcagg aaattgccaa
     4561 gtacccgatc atcacctacg acccggccta tagcggccgc tggaaggtga tggacgcgtt
     4621 caaaagagcc aaaattgagc cgaacatcat tttcggcgcg gtggacgcgg acgtgagcaa
     4681 aacctacgtg gagctcggaa tcggcatcgc gatcctcacc tcgatcagct tcgacaagta
     4741 ccacgatcgc ggcctgcgcg cccgcgatgc gagccatctt ttcgagccga gcacgacata
     4801 tgtg
//
LOCUS       DBNGWZ010000250        13662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_654916, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000250 DBNGWZ010000000
VERSION     DBNGWZ010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13662
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_654916"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..649)
                     /locus_tag="AC16CX_07465"
     CDS             complement(236..649)
                     /locus_tag="AC16CX_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012388.1"
                     /translation="MATTVRKTAYFSMKTPNRAGQGARLLNALAAHGVNLLAFTGFPN
                     AGRAQVDFIPYDISKFTRAARKLGMKVSKRKAVFLAQGTDKAGACAALCSKLAKSGIN
                     MVAMDAVAAGRGRFGAIFWVKPRDVAKASRVLRAR"
     gene            873..1766
                     /locus_tag="AC16CX_07470"
     CDS             873..1766
                     /locus_tag="AC16CX_07470"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3012389.1"
                     /translation="MDLPGKSSVTVIPTGTALGADVAGVDLSRPLVADLFKAIESAWH
                     QHLVLRFRGQTLDDPALLAFARRYGELDKAPVHAVGDAEVDPYPEITVMSNIKVNGKS
                     IGNLGHYEAEWHTDMSYNENTPIGSLLYSLEVPPEGGDTGFSNMYLAFETLPAELKRA
                     IIGKSCKHDSSRNSVGDLRKGFKEVTDPRAAPGAVHPLIRTHPATRRNALFLGRRRNA
                     YIVGLPLDESEELLNRLWAHAARPEFAWYQKWEVGDLVMWDNRCVMHRRDAFDPQSRR
                     LMHRTQIKGNKPFFDPATLAA"
     gene            complement(1809..2081)
                     /locus_tag="AC16CX_07475"
     CDS             complement(1809..2081)
                     /locus_tag="AC16CX_07475"
                     /inference="COORDINATES: protein motif:HMM:NF043076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HIR3012390.1"
                     /translation="MAQEPELPKDWLEFMQKMWNPMSYPMPGMAMPTVKVEEVEKKIA
                     ELKAVETWLTMQVGFVQMTVKTLEMQKAALESLTATVPKGKPESKK"
     gene            2226..2684
                     /locus_tag="AC16CX_07480"
     CDS             2226..2684
                     /locus_tag="AC16CX_07480"
                     /inference="COORDINATES: protein motif:HMM:NF040448.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HIR3012391.1"
                     /translation="MKFLKAVRLDDSDARILAGEGGAAEDGEWVVSGGYAVCDLAQGH
                     RTPRCHCDTTFIAAGARRRCTIAEVAEIDEASYEQVKHSLARHFLEELGAPTPDAARA
                     AAADECAYTADLAGGFPAEVWITVKREPTEDGVGERYSVFKRLMIGSHKL"
     gene            complement(2774..3163)
                     /locus_tag="AC16CX_07485"
     CDS             complement(2774..3163)
                     /locus_tag="AC16CX_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HIR3012392.1"
                     /translation="MARIRIAWNGGEVTANLRETPTVKQLVAALPCEASAHTWGEEVY
                     FELPLKAALETDARQVVEPGTVCYWVQGGSLALPFGPTPVSKGGECRLVTRCNVLGKI
                     EGDPRRLEKVKQGATIRVELMAEKRDK"
     gene            complement(3180..3947)
                     /locus_tag="AC16CX_07490"
     CDS             complement(3180..3947)
                     /locus_tag="AC16CX_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3012393.1"
                     /translation="MRIGSINPGSLRDIQVGPVARRFRKFVSILALFASVVALALIVE
                     SYYGQLLFGGSLQQPVDAISQADAGAKRSQQTALTPIEEGRYRALSEFVAKRYRVSQE
                     VAFDLVSVAHRVGEQLQLDPLLIIAVISVESRFNPIAESVKGAKGLMQIIPKYHTDKL
                     EEFGGERAVFDPETNIQVGAQILKEYIRLTGHLGAGLQMYAGALGDNDDQYTNKVLNE
                     KQRLQQVVTQSLRRGPAPAPVKTASVRNHTAFALPLD"
     gene            complement(4576..5037)
                     /locus_tag="AC16CX_07495"
     CDS             complement(4576..5037)
                     /locus_tag="AC16CX_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012394.1"
                     /translation="MESGTRRDAAPPAARGAGPAPSPDTAAKALPQPFQAAPRAAEQR
                     AAASPHAEDAAGGREAGAPAAATERTVPPTATGSPTEPARAKAMTRTTPAKPSGRGRV
                     PPWHGLEKEPPQKWLERIEELKRQEQAAEAEEMLAEFKRRFPDHPLPAGLQ"
     gene            complement(5472..6008)
                     /locus_tag="AC16CX_07500"
     CDS             complement(5472..6008)
                     /locus_tag="AC16CX_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3012395.1"
                     /translation="MLLYRDGDAGAFDALYARHKGGLYRYLLRQCHDAAAAEELFQDV
                     WMNLIRARAGYTVQARFTTYLYRLAHNRLIDHYRKNAQAVVTSFEDENCDALAELPDE
                     RERPAADALDAKRQAWHLLELIAGLPEAQREAFLLQQEGGMSVEEIAQATGVTRETAK
                     SRLRYAMAKLRQGMREWR"
     gene            complement(6215..7042)
                     /locus_tag="AC16CX_07505"
     CDS             complement(6215..7042)
                     /locus_tag="AC16CX_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012396.1"
                     /translation="MNWKAMAVAILSFSAAGTAGAVGNFADVTVYDRVENRTLPVHRH
                     AGRYYIVGKPGNEYQVRVRNRTGADILAVVSVDGVNAVSGETADWNQTGYVLGPHQGY
                     DVQGWRKSLERVAAFFFTDHANSYAARTGRPDNVGVIGVAMFRKKAEPEARLDQSPPR
                     AEPPASSARDDSPYPSSGDTASGAGSARSAESAAGPRHAPSPQAPQKSSSLGTGHGRS
                     MSSHVTYTSFERATASPEEVIAIHYDTYANLVAMGVIRAPRVALPSPFPGQFVPDPH"
     gene            7180..9234
                     /gene="fusA"
                     /locus_tag="AC16CX_07510"
     CDS             7180..9234
                     /gene="fusA"
                     /locus_tag="AC16CX_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3012397.1"
                     /translation="MPKFSPEAIRTIALAGHGGAGKTTLAEALLAKAGAINSPGSVEK
                     GTTVCDSDPLEKQYQHSLNASIVHLQHGDARVHLIDTPGLPDFIGRAIGALPAVETTA
                     IVVNAQNGIEMITSRMMQWARKRDLCRMIIVNKIDAENVDLPGLLAKLQAAFGKECLP
                     INLPADGGKRVVDCFFNPAGTADFSSVEEAHSALVDQVVEVDEELMAQYLEKGEVAPQ
                     ELHAPFEKALREGHLIPVCFVSARNGAGIAELLDIIVRLMPNPTEGNPPQLLKGEGAK
                     TQPIRAEPDPKKHVLAHVFKVMIDPFVGKVGVFRVHQGTITKDTQLFIGEGRKTFKVG
                     HAYLLQGKDFVETDALVPGDIGAIAKVEEIHFDAVLHDSHDEDQIHLVPLEFPTPMYS
                     LAIEPKRRGDEQKISDALHKLAAEDPTFKAEHNATVNQTIISGLGDLHMRYILDRMAN
                     QYHVEVSTKPPRIPYRETITAKAEGHHRHKKQTGGAGQFGEVFLRIEPLRRGSGFEFV
                     DQVKGGVIPNQFIPAVEKGVRQVLEAGPLAGFPVHDVRVIVYDGKHHEVDSKEVAFIA
                     AGKRAFMDALSKARAIILEPIVNIEITAPETSMGDIAGDLSSKRGQLSGTDSATPGTL
                     VIKGQVPLSELNNYQARLKSVTAGQGSYTIEFARYDPVPPTIQKDLVAAHAKTAAHAE
                     EQ"
     gene            9327..9935
                     /locus_tag="AC16CX_07515"
     CDS             9327..9935
                     /locus_tag="AC16CX_07515"
                     /inference="COORDINATES: protein motif:HMM:NF022609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HIR3012398.1"
                     /translation="MDEIVLRGMAKWPNVPSVYGWLALDRRGNWLIKGERVSNPLVTE
                     FIARNYARDERGCWFFQNGPQRVFVNLDYAPLVLRIASPEGALLAIEAHNGRPVASLS
                     GAWIDENGAILLETEYGPGTIHDSDLDRLLPCLTDATGNPLPEAVLDELMELAQQGKS
                     VPLWLKYRDSILKLEAIASRDVPRRFGFVARPEPPAGEEVCR"
     gene            9986..10780
                     /locus_tag="AC16CX_07520"
     CDS             9986..10780
                     /locus_tag="AC16CX_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR3012399.1"
                     /translation="MLPLSEFPIKERRAVRGVLFDIDDTLTTGGRLTAEAYAAMERLQ
                     AAGLTVIPITGRPAGWCDHIARMWPVAAVVGENGAFYFRYDDARRTMRRRFVDDAAMR
                     AAHRSRLGAIGEEILKAVPGAAIAADQLYRETDLAIDYCEDVARLPPEAVDRIVALMQ
                     AHGLTAKVSSIHVNGWFGAYDKLATTRSLMREAFGVDLEAARASFVFVGDSPNDEPMF
                     AFFPHAVGVANIREFAGRLAHEPRYVTTAAGGAGFREFADFILAAR"
     gene            10917..11948
                     /locus_tag="AC16CX_07525"
     CDS             10917..11948
                     /locus_tag="AC16CX_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HIR3012400.1"
                     /translation="MIDLRSDTVTRPTEAMLESMREATFGDDSRDGDETVRKLEALAA
                     GRTGKEAGAFMPSGTMANLVAMLTHGQRGGEVLLEDGSHTLNAELGGIAGVAGLFYRG
                     VPGKRGAMDLDALREMIRPVTRYYMGTALVWMENTHNRAGGAVLPLAHMQAVYGLARE
                     KEIPVHLDGARIFNAAISLGVPSSEIAQYSDSVCFCVSKGLSAPVGSILCGSAAFIER
                     ARAFRRMVGGNMRQAGPLAAAGIVALETMVDRLVEDHQTAKRLAEGLSRTDTSLCDPK
                     DVETNLVRVDVRKSGRRAAQWSADLKAKGVLVAPADIYVLRFVTHRHVSAPDIDTSVN
                     VFAELWRKN"
     gene            complement(12059..12271)
                     /locus_tag="AC16CX_07530"
     CDS             complement(12059..12271)
                     /locus_tag="AC16CX_07530"
                     /inference="COORDINATES: protein motif:HMM:NF025005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HIR3012401.1"
                     /translation="MDRKRVNASNIRSVGYDAGKQLLEVEFTSGSIVQYSGVSPEVNR
                     RFMSAPSPGSFFHDQIEENFPAERIR"
     gene            complement(12363..12839)
                     /locus_tag="AC16CX_07535"
     CDS             complement(12363..12839)
                     /locus_tag="AC16CX_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3012402.1"
                     /translation="MKASWIIALAILATTAFGNAAYAASAEVVIRSTKGEFKEVKERV
                     LLAIENRGLVLNYTARIGAMLERTGKDIGAARQVYADAELLEFCSARASRDTMEADPR
                     NIVFCPYSIAIYTLPKDRGTVYVAYRRPGAFGSEQSVKSLRAVEKLLADIVAEALK"
     gene            complement(12962..13309)
                     /locus_tag="AC16CX_07540"
     CDS             complement(12962..13309)
                     /locus_tag="AC16CX_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012403.1"
                     /translation="MNGESEEASRQEEGGEEKGGDGPAQARRAARRKAQGGKAPASAA
                     QARGEEAGGGQAGQPGSRSDGPAAGRYPRSLAIPDGLKALVIPDASRRITFAHSSGRL
                     TPPLNARLPELQM"
     gene            complement(13355..>13662)
                     /locus_tag="AC16CX_07545"
     CDS             complement(13355..>13662)
                     /locus_tag="AC16CX_07545"
                     /inference="COORDINATES: protein motif:HMM:NF021873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR3012404.1"
                     /translation="GSSLLNRRYRRRRQSMMHTITECRAESNYTLWLRFDDGLEGSVN
                     LGDLVLTESYRALSDEDIFSRVAIDPVANVVTWGGGIKLDPEVLYRDLASTTHAARH"
BASE COUNT         2364 a         4464 c         4352 g         2482 t
ORIGIN      
        1 gcgcacccgc acctggccct cgccgggctc gccgacctcc acctgctccc agcgcagttt
       61 gtcgggcccg ccgtattcgt gcacacgcat cgcgtaaggc atggtttctc cttttttgtc
      121 gggaatcgga gcgcgtgatt aaaccacatt tcccaagaaa acggggggcc gcagcagccc
      181 ccccgttttc tttactcgag catactgtct ctttggcgtt cttggcggtc gagcttcaac
      241 gggcgcgcag cacccgcgat gccttcgcga cgtcgcgcgg cttgacccag aagatcgcgc
      301 cgaagcgccc cctgccggcg gcgaccgcgt ccatcgccac catgttgatg ccggacttgg
      361 cgagcttgct gcagagcgcg gcgcacgctc cggctttgtc ggtgccctgg gcgagaaaca
      421 ccgcctttct cttgctcacc ttcatgccca gcttgcgcgc ggcgcgcgtg aacttggaaa
      481 tgtcgtaggg tatgaagtcc acctgcgcgc gtccggcatt gggaaagccg gtgaaggcga
      541 gcaggttaac gccgtgggcg gcaagggcgt tcaacagtct cgcgccctgg ccggcgcggt
      601 tgggggtctt catgctgaag taggcggtct tgcggacggt ggtggccatg gttgccctcc
      661 tttggacgtg cggcttgttt cggaattcaa cccttagcga tacgaccacc ggttaagcgg
      721 caaccctctg tccgccttga accggtggac ttaatccggc gcacttggcg tccggattaa
      781 gtgaaattat gcgccgctcc ccgcatgaaa gcatccggct tgcctggcga cattcctggc
      841 attagcattt ctgaaaacgg gaggaacccc ccttggattt gcccggcaag agcagcgtca
      901 ccgtgatccc gaccggcacc gcactgggcg cggacgtcgc gggcgtggac ctgtccaggc
      961 cgctggttgc cgacctgttc aaggccatcg aatcggcgtg gcaccagcac ctggtgctgc
     1021 gattccgcgg tcagactctg gacgacccgg ccctgcttgc cttcgcgcgc cgttacggcg
     1081 aactcgacaa ggccccggtc cacgccgtgg gagacgccga ggtggatccc taccccgaga
     1141 ttaccgtgat gtcgaacatc aaggtaaatg gcaagtcgat cggcaacctc ggccactacg
     1201 aggccgagtg gcacaccgat atgtcctaca acgagaacac ccccatcggc agcctgctct
     1261 attcgctgga agtgcccccc gagggcggcg ataccggctt ctccaacatg tatctcgcct
     1321 ttgagaccct ccctgcggag ttgaaacgtg cgatcatcgg caagtcctgc aagcacgatt
     1381 cgagccgcaa cagcgtcggc gatctgcgca agggcttcaa ggaagtgacc gacccgcgag
     1441 cggctccggg agcagtgcat cccctcatcc gcacccatcc ggcgacgcgc agaaacgcct
     1501 tgttcctcgg gcgccggcgc aacgcctata tcgtggggct gccgctcgac gagagcgaag
     1561 aattgctgaa ccgcctgtgg gcgcacgcag ccaggccgga attcgcctgg taccagaagt
     1621 gggaggtcgg agacctcgtg atgtgggata accgctgcgt gatgcacagg agggacgcgt
     1681 tcgatccaca gtcccgaagg ctcatgcata ggacccagat caaaggcaat aagccgttct
     1741 ttgatccagc gacgctcgcg gcatagcggg tctcggatgc cacgacaggc ggactgacgc
     1801 cggccgtctt attttttgct ctccggcttc cccttcggca ctgtggcggt aagcgattcg
     1861 agcgcggctt tctgcatctc gagggtcttc accgtcatct gcacgaatcc cacctgcatg
     1921 gtgagccagg tttccaccgc cttcagctcg gcgatctttt tctcgacctc ctccaccttg
     1981 acggtgggca tcgccatgcc gggcatggga tacgacatcg gattccacat cttctgcatg
     2041 aattccagcc agtctttcgg cagttccggt tcctgtgcca tgtagcgctc ctataccgcg
     2101 gccgtttccg cgcctattat agggtcaatg cggacggtga accgccgctt gacccgcgcg
     2161 agttcgagca gacgactatt ccaccgctca actcgcgctc atgaagtgcc ggtacggggt
     2221 tcgaggtgaa atttctcaag gcggtgcggc tcgacgattc cgacgcccgt atcctcgccg
     2281 gggagggggg cgccgcggag gacggcgagt gggtggtttc gggaggctac gcggtgtgcg
     2341 acctcgcgca aggtcacaga acgccgcgct gccactgtga cacgaccttt atcgccgccg
     2401 gtgcgcgccg ccgctgcacc atcgccgagg tggccgagat cgacgaggca tcctacgagc
     2461 aggtgaaaca ttcgctggca cggcatttcc tggaggaact cggcgcgccc acgcccgacg
     2521 ccgcgcgtgc ggcagcggcg gatgaatgcg cctacaccgc cgatctggcc ggaggcttcc
     2581 ccgccgaagt ctggatcacg gtcaagcgcg agccgaccga ggatggcgtg ggggagcgct
     2641 actccgtctt caagcgcctg atgatcggct cacacaagct gtgaggaatg aaggcgaaag
     2701 acgaagggcg tgataggtga taagtgatag gtagcaactc ccgctgagcg ggaaacttgt
     2761 cacttatcac tcgtcactta tcacgcttct ccgccattag ctcgacgcgg attgttgcgc
     2821 cttgcttgac cttctcgagc cgccgcgggt cgccctcgat tttccccaat acgttgcacc
     2881 gggtgaccag ccggcactcg ccgcccttgg aaacgggagt cgggccaaac ggcagcgcga
     2941 gcgaaccgcc ctgcacccag tagcacaccg tgcctggctc gacgacctgc cgggcatcgg
     3001 tttccagggc agccttcagc ggcagctcga aatagacttc ctcgccccag gtatgagcgc
     3061 tcgcctcgca aggcagcgcg gcaactaatt gttttaccgt gggagtttcc cgcaagttag
     3121 cggttacttc accgccattc caggcgatcc gtatgcgcgc cacgaggagc gggtgccgct
     3181 tagtcgagcg gcagggcgaa ggcggtgtga ttgcggacgg atgcggtttt gaccggggcc
     3241 ggcgccgggc cacgtcgcag agactgggtc acgacctgtt gcagtcgctg cttctcgttc
     3301 agcaccttgt tggtgtactg gtcgtcattg tcgccaagcg cgccggcgta catctgaagc
     3361 ccggcgccga ggtgaccggt gagacggatg tattccttca ggatctgtgc cccgacctgg
     3421 atgttggttt ccgggtcgaa taccgcccgc tcgccgccaa actcctcgag cttgtcggta
     3481 tggtacttcg ggatgatctg catcaatccc ttggcgccct tcacgctttc agcgatcggg
     3541 ttgaagcggg attcaaccga gatcacagcg atgatgagca agggatcaag ctggagctgc
     3601 tcgcccacgc ggtgcgccac actgacgagg tcgaaagcga cctcctggga cacccgatag
     3661 cgtttggcca cgaattcgga gagggcgcgg tagcggcctt cctctatcgg ggtgagggcc
     3721 gtctgctggc tgcgtttggc gccggcatcg gcctgcgaaa tggcgtccac cggttgctgc
     3781 agggaccccc cgaacaagag ctgcccgtag tagctttcca cgatgagggc cagagccacc
     3841 acgctggcaa acagcgccag gatggagacg aatttacgaa accgccgggc gacagggccg
     3901 acctgtatgt cccgaaggct gcccgggttg atgctaccaa tgcgcacgat gactgccttt
     3961 cccttgttcc ggcatttcgg ttgtgccggc caagtattgg ttcgtcgcgg cagcaacacc
     4021 cctcttctga gaaccggtcc agttccgacc actataagtt gtggcgctct tcgaaaaact
     4081 ggctaattct agtggttcag gtaggcgcct gcaacggttt catcacttcg ggcggctatc
     4141 ttgaccctat ttagagggat ttggtgcgac gcagtgggcg cgaattctgc tttcttgata
     4201 ttgttttgtc aaatatttat gacaaacaat aaagcagaaa tgctgcggcg cattacgact
     4261 cctgccagat cactggcaga attggacagg ggttcgttcc cggcgttccg attgaactac
     4321 gttaatctat ataaaacagt atgttatata agacgccccg actcaaccgg gtgagggatc
     4381 gggggcgtct tctggatgac gctccagcca ggcgctgagt gcatcgtgat aacgctccaa
     4441 ggcctcgcga gcagtagaac acgcaggggc tcacaacccc tcttgcagca ttcctccagt
     4501 gctggctcct gtgggggtag cgggcggggg tcggcgaccg gtggcatggg tggtctatct
     4561 agtcagctca tccgcctact gcagcccggc tggcaaggga tggtcaggga agcggcgttt
     4621 gaactcggcc aacatttcct cggcttcggc tgcctgttcc tgccgcttca gttcctcgat
     4681 gcgctcaagc cacttctgcg gcggttcctt ctccaagcca tgccacggcg gcacccgccc
     4741 ccgccccgag ggcttcgccg gggtggtccg cgtcatggcc ttggcgcgtg ccggctcggt
     4801 cggggaaccg gtggccgtcg gtggcacagt ccgttcggta gctgcggccg gtgcaccagc
     4861 ctctctgcct cccgccgcat cttccgcatg aggcgacgcg gcggcacgct gttcggccgc
     4921 gcgtggcgcg gcctggaacg gctggggcaa cgccttggcg gcggtatccg gcgatggcgc
     4981 cggccccgcc ccgcgcgcag cgggtggagc cgcatcccgg cgcgtaccgc tttccatctt
     5041 gcccgagccc gctgtcgcgc cggagcgagc gtcatcccgc gatgcaggac gtgcgggcgc
     5101 gggggcagcg gctcgcggtt gcgttttggt tggttcgatc ggtgccggcg gcgccggttc
     5161 cactggctga actgcgggcg gtgctgcggg cgggggcgga gcgtgcagga gcggctcacc
     5221 gccctcttcc ctgaccagca tgaccaggct cacgctcaat acgacaaccg ccgcgatcga
     5281 aactggcacg cgccaccggt gcagcgccga gtacagagca cgcggtcgtg cgccgacctc
     5341 ccggcgcgcc gcggcgagaa tcgcggcgtc gaggtgcgcg gggggtgctt cgcgcgcagc
     5401 ttcccggtat aggcggtcaa gccgcggatc gcgctctgcg cccgccggcg caccacccgt
     5461 cttgctgtcg ttcagcgcca ttcgcgcatc ccttgtctca acttggccat cgcgtagcgc
     5521 agccggcttt tcgcggtttc gcgcgtgaca ccggtcgcct gcgcaatctc ctccacgctc
     5581 atgccgcctt cctgctgcag caggaaggcc tcgcgctgcg cctcgggcag tccggcgatt
     5641 agctccagca ggtgccacgc ctgccgcttt gcgtcgagtg catcagccgc cggccgctcg
     5701 cgctcgtccg gcagctccgc cagcgcatcg caattctcgt cctcgaagga ggtcacgacg
     5761 gcctgggcgt tcttgcggta atggtcgatc aaccggttgt gcgccagccg atagagatag
     5821 gtcgtgaatc tggcctgcac cgtgtatccg gcccgcgccc ggatgaggtt catccagacg
     5881 tcctggaaca gctcttcggc tgccgccgcg tcgtggcact gccgcaacag gtagcggtac
     5941 agcccgccct tgtggcgcgc gtagagcgcg tcgaatgcgc ccgcatcgcc atcgcgataa
     6001 agaagcatga gctcttcatc gctgctctcc gcgccccagg acataaccgg ttgccagtat
     6061 gcgcaacgcg cagcgggcga gcaacccgcc gcgcaatgcg ccgagaggcg tgaggcgaga
     6121 agagtgagga gcaaaatctc ttctcaccat aacgggagcg aagcgcgaac tgacgcctcc
     6181 cgcctcactc ctcacccctc actcccggcc cgcgtcaatg cgggtcaggg acgaactgtc
     6241 ccggaaacgg cgacggtagc gcaacgcgcg gcgcccggat cacgcccatc gccaccaggt
     6301 tcgcgtatgt gtcgtaatgg attgcgatca cttcctcggg gctggcggtc gcccgctcga
     6361 agctcgtgta ggtcacgtgc gagctcatgc tgcggccatg cccggtaccg agcgaggatg
     6421 acttctgcgg ggcttgcggt gacggcgcat ggcgcggccc ggctgcgctc tccgcggaac
     6481 gggcgctgcc ggccccgctt gcggtgtcgc cggaagacgg gtacgggctg tcatcgcgcg
     6541 cgctcgaagc cggcggctcg gcgcgcggcg gcgattggtc cagccgcgcc tccggctccg
     6601 cctttttccg gaacatcgcg accccgatca cgccgacgtt gtccggccgc ccggtgcgcg
     6661 cggcgtagga gttcgcgtga tcggtgaaga agaacgccgc gacccgctcg aggctcttgc
     6721 gccacccctg gacgtcgtag ccctggtgcg ggcccaggac gtagccggtc tggttccagt
     6781 ctgcggtctc gccgctcacc gcgttgactc cgtccaccga caccacggcg aggatgtcgg
     6841 cgccggtgcg gttgcgcacg cggacttggt actcgttgcc gggcttgccc acgatgtagt
     6901 agcggcccgc gtggcgatgg acgggcaacg ttcggttctc gacccggtca taaaccgtca
     6961 catccgcgaa attgccgacg gcgcccgccg tgccggctgc gctgaacgaa aggatcgcga
     7021 ccgccatcgc cttccaattc atctttgtct ccttgaggtt aagcatcgaa ccgatgctca
     7081 accttataac ggtgcgtcgg cctggatggg gttagcttga aggagacttg gcccgccgtt
     7141 atactcgacc aacccccggc aagagggtcg aggagttcca tgcccaaatt ttcccctgaa
     7201 gccatccgca ccatcgccct cgccggccac ggtggcgcgg gcaagaccac cctcgccgag
     7261 gcgttgctcg ccaaggccgg agcgatcaat agcccgggca gcgtggaaaa gggcaccacg
     7321 gtctgcgatt ccgacccgct cgagaagcag taccagcatt cgctcaatgc ttccatcgtt
     7381 cacctgcagc acggcgacgc gcgcgtgcac ctgatcgaca ccccggggct gcccgacttc
     7441 atcgggcgcg cgatcggggc gctgccggcg gtggagacca cggcgatcgt ggtcaacgcc
     7501 cagaacggca tcgagatgat cacgagccgc atgatgcagt gggcgaggaa gcgcgacctc
     7561 tgccgcatga tcatcgtcaa caagatcgac gccgagaacg tcgacctgcc ggggctgctc
     7621 gcgaagctgc aggccgcctt cggcaaggaa tgcctgccga tcaacctgcc cgccgacggg
     7681 ggcaagcgcg tggtggactg cttcttcaat cccgccggta cggccgactt ctcctcggtg
     7741 gaggaagcgc acagcgcgct ggtggaccag gtggtcgagg tggacgagga gctgatggcc
     7801 cagtacctcg aaaagggcga ggtggccccc caggaactgc acgctccgtt cgaaaaggcg
     7861 ctgcgcgagg ggcacctgat tccggtgtgc ttcgtctcgg cgcgcaacgg cgccggcatc
     7921 gccgagctgc tcgacatcat cgtcaggctg atgcccaacc ccaccgaggg caatccgccg
     7981 cagttgctga agggcgaagg cgccaagacg cagccgatcc gcgccgagcc cgacccgaag
     8041 aagcacgtgc tcgcgcacgt gttcaaggtg atgatcgacc cgttcgtggg caaggtcggc
     8101 gtgttccgcg tgcatcaggg cacgatcacc aaggacaccc agctcttcat cggcgagggc
     8161 aggaaaacct tcaaggtcgg ccatgcctac ctgctgcagg gcaaggattt cgtcgagacc
     8221 gacgcgttgg tgccgggcga catcggggcg atcgcgaagg tggaggagat tcatttcgac
     8281 gccgtgctgc acgattcgca cgacgaggac cagatccatc tcgtgccgct cgagttcccg
     8341 accccgatgt actcgctcgc gatcgagccc aagcgccgcg gcgacgagca gaagatctcg
     8401 gacgcgctgc acaagctcgc tgccgaggat ccaaccttca aggccgagca caacgccacg
     8461 gtcaaccaga ccatcatcag cggactgggc gacctgcaca tgcgctacat cctcgaccgc
     8521 atggcgaacc agtaccatgt cgaggtctcg accaagccgc cgcgcatccc ctaccgcgaa
     8581 acgatcacgg cgaaagccga gggacaccac cggcacaaga agcagaccgg gggcgcggga
     8641 cagttcggcg aggtgtttct caggatcgag ccgctcaggc ggggatcggg cttcgagttc
     8701 gtggaccagg tgaagggcgg ggtcattccc aaccagttca tccccgcggt ggaaaagggc
     8761 gtgcgccagg tgctggaagc gggcccgctc gcgggtttcc cggtgcacga cgtgcgcgtc
     8821 atcgtctacg acggcaagca ccacgaagtg gactcgaagg aagtcgcgtt catcgcggcg
     8881 ggcaagcgcg cgttcatgga cgcgctgagc aaggcgcgcg cgatcatcct ggagccgatc
     8941 gtcaacatcg agatcaccgc gcccgaaacc agcatgggcg atatcgccgg cgacctctcg
     9001 tccaagcgcg gccagttgag cggcaccgat tcggccacgc cgggaaccct ggtgatcaag
     9061 ggccaggtgc cgctctccga attgaacaac tatcaggcgc ggctgaagtc ggtcaccgcc
     9121 ggccaaggct cgtacaccat cgagttcgcg cgctacgatc cggtgccgcc cactatccag
     9181 aaggacctgg tggccgccca cgccaagacc gcggcgcacg ccgaggaaca atgaacgcgt
     9241 gatccagctt ctgcgaccgt ttcccggcta ctgctaacac agtcctttct gcaagcccat
     9301 ccattcgctc gttcacgcgt tcattaatgg atgagatcgt cctgcgcggg atggccaagt
     9361 ggcccaacgt gccgtccgtt tacggctggc tcgcgctcga ccggcgcggc aactggctga
     9421 tcaagggcga gcgcgtcagc aatcccctcg tcaccgagtt catcgcccgc aactacgcgc
     9481 gcgacgagcg cggctgctgg ttcttccaga acggcccgca gcgggtgttc gtcaacctcg
     9541 attacgcacc gctcgtgctg cgcatcgcca gccccgaagg cgcgctcctc gcgatcgagg
     9601 cccacaacgg cagaccggtc gcaagcctga gcggggcgtg gatcgacgag aacggagcaa
     9661 tcctgctcga gactgagtac ggcccgggca cgatccacga cagcgatctc gaccggctgc
     9721 tgccgtgtct gacagacgcc accggcaatc cgctgccgga agcggtcctc gatgagctca
     9781 tggaactggc ccagcagggg aaatccgtgc cgctatggct gaaataccgc gattccatcc
     9841 tgaagctcga ggcgatcgcg tcgcgcgacg tcccgcgccg gttcggtttc gtcgcccggc
     9901 ccgagccgcc cgccggtgaa gaagtgtgcc gatgaacggc tgcgcgaccg cttgccgttc
     9961 ataaattgaa gatcggaata tcttcatgct gccattgagt gaattcccaa taaaagaacg
    10021 ccgcgccgtg cgcggcgttc ttttcgatat cgacgacacg ctgacgacgg gcggccggct
    10081 caccgcggag gcctacgcgg cgatggagcg cctgcaagcg gccgggctga ccgtcatccc
    10141 gatcacgggc cggcccgccg gatggtgcga ccacatcgcg cgcatgtggc cggtcgcggc
    10201 ggtagtgggg gaaaacggcg ccttttattt ccgctacgac gacgcccgcc gcacgatgcg
    10261 gcgccgtttc gtggatgacg ccgcgatgcg cgccgcccac cgctcgcggc tcggcgcgat
    10321 cggcgaggaa atcctgaagg cagtcccggg cgccgccatt gccgccgacc aactctatcg
    10381 cgagaccgac ctcgccatcg actactgcga agacgtcgcg cggctaccgc ccgaggcggt
    10441 ggaccgcatc gtcgcgctga tgcaggcgca cggcctcacc gccaaggtga gctcgatcca
    10501 tgtcaacggc tggttcggcg cctatgacaa gctcgccacg acccggtccc tgatgcgcga
    10561 ggccttcggc gtcgacctcg aggcggcgcg cgcgagcttc gtcttcgtcg gcgactcccc
    10621 caatgatgag ccgatgttcg cctttttccc gcacgcggtc ggggtcgcca acatccgcga
    10681 gttcgcgggc cggctcgcgc acgagccgcg ctacgtgacc accgcggccg gtggggcggg
    10741 ctttcgcgag ttcgccgact tcatcctcgc ggcacgctaa ctgcaacttc ctgaaccacg
    10801 aaggacacaa aggacacgaa gatctacttc catcaacaat tctgtctttc ctttgtgtat
    10861 ttggtgtcct ttgtggttta agattttggt atcccagcga cagcggttga tctgccatga
    10921 tcgatcttag gagcgacacc gtcacccgcc ccaccgaggc gatgctcgaa tccatgcgcg
    10981 aggccacctt cggggacgac tcgcgcgacg gcgacgaaac cgtccggaag ctcgaggcgc
    11041 tcgccgccgg ccgcaccggc aaggaggccg gcgccttcat gccgagcggc accatggcga
    11101 acctggtcgc gatgctcacg cacgggcaac ggggaggcga agtgctgctg gaggacggct
    11161 cgcacacgtt gaacgccgag ctaggcggca tcgccggcgt ggccggcctg ttctaccgcg
    11221 gcgttccggg caagcgcggc gcaatggacc tcgacgcgct gcgcgagatg atccgcccgg
    11281 tcacccgcta ctacatgggc acggcgctgg tgtggatgga gaacacccac aaccgcgccg
    11341 gcggcgcggt gctgccgctg gcgcacatgc aggcggtcta cgggctcgcg cgcgagaaag
    11401 agatcccagt gcacctcgac ggcgcgcgca tcttcaacgc agcaatttcg ctgggcgtcc
    11461 catccagcga aattgctcaa tattcagatt cggtgtgttt ctgtgtctcg aaggggctgt
    11521 ccgcgcccgt gggatcgata ctctgcggga gtgccgcatt tatcgagcgc gcccgcgcat
    11581 tccgccgcat ggtcggcggc aacatgcgcc aggcagggcc gctcgccgcc gccggcatcg
    11641 tcgcgctgga aacgatggtg gaccgcttgg tcgaggacca tcagaccgca aagcggctcg
    11701 ccgagggact gagtcgcact gacacaagcc tttgcgatcc gaaagacgtg gagaccaacc
    11761 tcgtgcgggt ggacgtgagg aaaagcggcc gccgcgcagc gcagtggtcg gcggatctca
    11821 aggcgaaggg cgtgctggtc gcccccgccg acatctacgt cctgcgcttc gtcacccacc
    11881 gccatgtctc cgccccggac atcgacacgt cggtcaacgt cttcgccgaa ctctggagga
    11941 aaaattagaa ttttcccgca cgagtgccgt tccaactatc gtgggctgac cgcagcagtc
    12001 gcaatatggc agcgcgttta tatatgagct gcaataggcg caaaaagttg gaacggcact
    12061 agcgaatgcg ctccgcggga aaattttcct cgatctggtc gtggaagaag ctgccgggcg
    12121 agggcgcgct catgaagcgg cggtttactt ccggcgagac gcccgagtac tgcacgatcg
    12181 agccgctcgt gaattccact tccaggagct gcttgcccgc gtcatagccg accgagcgta
    12241 tgttggaggc gttgacgcgc ttgcggtcca tcaagagccg tgaatggtaa atcgtgaatg
    12301 gtgaatagaa aaaactttgg tttcctttgt tttcttggcg tcttggcggt aaaggtctgg
    12361 ggttacttga gggcttcggc gacgatgtct gcgagcagct tctccactgc ccgcagcgat
    12421 ttcaccgatt gctcggagcc gaaagccccc gggcgccggt acgcgacgta aaccgttccc
    12481 ctgtccttgg gcagcgtata gatcgcgatc gagtacgggc agaatacgat gttgcgcggg
    12541 tcggcttcca tcgtgtcgcg ggaagccctg gcgctgcaga actcgagcag ctcggcgtcg
    12601 gcataaacct gccgcgccgc cccgatgtcc ttgccagtgc gttcgagcat cgcgccgatg
    12661 cgcgcggtgt agttgagcac gaggccgcgg ttctcgatcg cgagcagcac gcgctccttc
    12721 acctctttga actcgccctt ggtcgagcgg atgacgactt ccgcagacgc agcgtatgcc
    12781 gcgttgccga acgcggtggt tgcgagaatt gccagcgcaa tgatccatga tgctttcatg
    12841 ctttcctccg ggttggatgt ccgggactta cgcccggtcc ggcggttgat tctacggagg
    12901 cagattacgg aagttgttgc actagatcaa cagcgctcga tggccctgcg gttgattcat
    12961 gttacatctg gagttccggt agccgcgcat tgagcggcgg cgtcagccgt ccgctcgaat
    13021 gcgcgaaagt aatccggcgc gaagcgtccg gtattactag ggctttgagc ccatcgggaa
    13081 tggccaggct ccggggataa cgaccggccg cggggccatc ggagcggctg ccgggctggc
    13141 cggcttggcc gccgccggct tcctcgccgc gcgcctgggc cgcgctggcc ggcgccttgc
    13201 cgccttgcgc tttccgccgc gccgcgcggc gggcctgcgc cgggccatcc ccgccttttt
    13261 cctcgccgcc ttcttcctgc cggctcgcct cttcgctttc gccattcaat cctccagtcg
    13321 gttacccccg cgcggcgaat cccgaggagg gctcctagtg acgggccgcg tgggtcgtgc
    13381 tcgccaggtc ccggtacagc acttccgggt cgagcttgat gccgccgccc caggtcacca
    13441 cgttcgccac cgggtcgatt gcgacgcggc tgaagatgtc ctcgtcggaa agcgcccgat
    13501 acgactcggt cagcaccagg tcgccgagat tgacgctccc ctccagcccg tcgtcgaaac
    13561 ggagccacag ggtgtaattg ctctcggcac ggcattcggt gatggtgtgc atcatgctct
    13621 gtctgcgtct gcggtaacgc cggttcagca ggctggaacc ca
//
LOCUS       DBNGWZ010000251         4127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3049069, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000251 DBNGWZ010000000
VERSION     DBNGWZ010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3049069"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          749 a         1341 c         1244 g          793 t
ORIGIN      
        1 ctggaaagga cctcctgctg gtgtgcatcc tgcgcggcgg cgtgatcttc ctcaccgacc
       61 tcatgcgcca gatcaccatc ccgcacgcga ttgacttcat ggccgtgtcg tcctacggcg
      121 tcggcgcacg cacctccacg ggcgaagtcc gcatcgtgtt cgatctgaac cagagcatcg
      181 aaggccgcca cgtattgatc gtcgaagata tcgtggacac cggccacacc atcgccgccg
      241 tgatcgacat gcttgccacc cgtcaccccg cctcgctgag aatctgcgcg ctgctcaaca
      301 aggccgagcg ccgcgaagtg accgtgccta tggactacgt cggcttcacc atccccaaca
      361 aattcgtctt cggctacggc ctggacattg acgagcacta tcgctacctg cccttcatcg
      421 gcgtcgtcaa gccggacgtg tatctgaagt gaaatgagcg cagcattttt tgcgcgtcca
      481 gcctccgtgc cgtatactgg gaaggagccg acggaaccat ccttcacgcg ctgccgcggg
      541 ggaagttgtg acgcatctgc tcaatgacca gcaacgccgc gcgtacgccg ggcgctgggt
      601 cgcgctggtg caggggcgcg ttgccggggt cgggctgacg gccgaggccg cccaactggc
      661 cgcgcggctc tcccgcccca aagaaaaatc tcaggtcatt ttcgtgcccg aacgtcccga
      721 gctcgcattc ccccctctcc tccacgacat ccgccgtgcc ctgcctgccg atgccgaagc
      781 ctggctggtg ggcggcgcgg tgcgcgatgc gctgctgaac cggcgcacca gggatttcga
      841 tttcgccgtg gcgggcgacg ccctcgccgc cgcccgcaaa gtggccgacg cgctccacgc
      901 cgcgttctac cccctcgacg ccgggcgcga cgtaggccgt gtcatcgtca cgcgcgacga
      961 agtccgcttc acccttgact tcgcgcgcct gcgcggcccc gatctcgccg ccgatctcgc
     1021 cgcccgcgac ttcaccatca acgccctggc cgtctcgctc gccggtctcg aagagctcat
     1081 tgatccatta catggggaag aggacctgcg ggcgaagatc gttcgcgcgt gttcgccgca
     1141 ggccgtcgcc gacgacccca tccgcggcat ccgcgccatc cggctcgccg cgcagctcga
     1201 tttccggatt gatagggaaa cgcgcgccgc cgtccgcgcc caggctggcg cgctcgcgca
     1261 tacctccccg gagcggcggc gcgacgagtt catccgctgt ctcggcggtc cgcgtgcggc
     1321 tgcgtctctg cgggcgttag accttctagg cctcttagag catctcgtcc cagaagccct
     1381 cgcgctcaaa ggcgtcacac aatccccgcc gcacgagctt gacgcctggg aacacacgct
     1441 tgccactgtg gcctgcctcg acgaagtgct cgacgtgctc gggccggtac acgacgtgga
     1501 caccgcctcc gatctcacgc tcggcctcgt ctcactccgc ctgggccgct tccgggggca
     1561 actcttcgag cacctcagca cgctgctcag cggcgaccgg cctgcgcgct ggctgctgat
     1621 gctggcggca ctcctccacg acatcggcaa gccgcagacg cggacggtcg agccgcgcga
     1681 agcgggccgt atccgctttt tcaatcacga gaccgtcggc gcggagatgg ccgcccatcg
     1741 ccttgcccgc ctccacttca gcgccgacga agtcaggcgc gtccggatca tcaccgccca
     1801 ccacatgcgc ccccgccaga tgtcaaaagg gcagcccccc acccgccgtg cgatctaccg
     1861 cttctatcgc gacacaggcg aagccggggt ggacattgtc ctgctctcgc tcgcggactt
     1921 cctcgccaaa accaatagcg gcccgccgcc gcaggatgag tgggccggac acctcgacgt
     1981 gtgcgcgcag ttgctgcacg catacttcga gcggccccag gaggccgtcg cgccgcccgc
     2041 cctcatcacc ggccacgatc tgatcgccga gttcggactg gaagccgggc cgctcatcgg
     2101 taagttgctc gaggccgtgc gggaggcgca agccgcgggc gaaatcgccg accgcccggg
     2161 cgcgctggac tacgtccggc aacacctggc cgataaataa aagcggcccg ctccagagag
     2221 cgggccgctg cggttttgat tcgccgttta ggcgctgcgc gatatccatt caaccctcac
     2281 gagcggagac gcgccactat agtccccgaa gcgaagcgga gtgggatggc gcgtctctac
     2341 tacggataaa tttccgtcaa taccggctcg taggagcact tgcaggaggg aacacacgaa
     2401 cagccttccg gcggcaagac cggaactgca tcttttggat aggcgccctg aagagactgg
     2461 cacaccggac aggcgtcgtg gtacacggag acgcgcacca ggctgatccg gtcgttgcgc
     2521 ttcatctttt ccaaggtttg ggataattca gccgctttgg gcatggaagc cctcctcgca
     2581 aaattgacga ccagatttta gcaaggactg tcgcccaatg caagcgagtc gcgattgaca
     2641 acggttttga gaagcaggtt gacgaagctc actgtctgca tataattcga tgtgcagtgg
     2701 cccattagct caatggtaga gcagctgact cttaatcagt tggttggggg ttcgagtccc
     2761 tcatgggtca ccaaagaaaa aggtcctttt cctagggcct ttttcgattc gactcatatt
     2821 cttgccgagg gctcgaatca tgccggtatg tatccaatgc ggtgaagaaa aggataaggg
     2881 tttctaacgg cggcccaaga gatggttgga aaccttggat aaggcactgt tggcacaacc
     2941 ggtagtcagc ggccttcagc cttgcacgcc cggtgtattc gtctaaaagc agagccgcct
     3001 tctttcaggc ggctctggcc ttgcagtgaa aacaaacagc ttgcctgctc tacaggtttt
     3061 gttgagccag ctttctattc gctttttgct gaccggatgc ctatgggcca accgttgtaa
     3121 agaatgcatc cgtgcgattg gccgcattgc cattgttcac ttgaatgaac accggcagga
     3181 aggtgttccc cctgctggtc aggccttcgt aatcgccgag gaagaagcca aacggcccgc
     3241 gcgcagcagg cgcttgctcg acgtcgaatg acgccgacga aaggcgcgct tcgtcacccc
     3301 agtttgcagc attcgaacag tcaaccgtcg tggcgtgaca gtgaacgatc cagtaatcgg
     3361 tcggcgcgcc ggggttcgcg tcgttgttgc ggaaatcata gtaagtgaca gcgatggtgc
     3421 catccgccgc aacgtgaacg actgggacga aggcctgttg attgagtgga ttcatattcg
     3481 caggcgtctg attgaccctg accggcgtcg accaggtatc gccgccgtcg gtggacattg
     3541 agaaggcgat ctcgtcgacg cccctgaagc gcaagtcctg ccacaccgcg tagaggttgc
     3601 cgttgttgcg gtcgacagcg acatcgggga tgaggccttc agcccggatc ggtctgccag
     3661 tgtcggggtc gagcgcaccc tggaagaact ccttggctgc gcgcactacc ttgccccact
     3721 tggcgccttt gtccttggat cgaacgaggg agaggctgac atcgaattgg gtgcctccat
     3781 cgctgttctt gaattccagg aattcgctaa aaaagttgac cagagtcccg ttgggccgca
     3841 cgacgatctg gttgccgatc gtggcgttgt tcgcgcccgg gttgtagatg atccgcgctg
     3901 gctcccaggt ctggccggcg ttcgtggttc gcgagaacag cgctggcccc ttggtaccga
     3961 cgaggaggtc aaacgcccga ttgactggaa atcccggcgg gacatcggag cgatcccaaa
     4021 cggcgtagac gtagtttgag tcggtcgggt cggcggtgat tgactccttg tcgttgaaga
     4081 agcgcgggtc ggtatcccgg atcagcgtga tcggctcgct ccaggtg
//
LOCUS       DBNGWZ010000252         2922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_403640, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000252 DBNGWZ010000000
VERSION     DBNGWZ010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_403640"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="AC16CX_07575"
     CDS             complement(<1..959)
                     /locus_tag="AC16CX_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012405.1"
                     /translation="MSLRLAAAVLLAVVLAAPAGALAQKYPAKTIRLVLPFTAASAVD
                     VLARLYAQKMAETWGQQVLVDNRTGAAGIIGMEAIARAAPDGYTLGMGNAATLAMNPH
                     LYQKLPYDVTRDYVPILQAAVIRNCLVVHPSLPAKSVKELVALAKARPGQLNYASGGV
                     GSAQHVPMEMLKSLAGINIVHVPYKGLTPAFNDVLAGEMHMIIPGLVTALPFHKTGRL
                     RIIATTGATRTSVTPEIPTIAQAGVPGYEFDSWTGFIAPTGTPPAIVSQLHGELARIS
                     KLPDIRERLTAMGFDVVGGSPDAFAAVIKDNNARLGKVIREAG"
     gene            complement(1039..2025)
                     /locus_tag="AC16CX_07580"
     CDS             complement(1039..2025)
                     /locus_tag="AC16CX_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012406.1"
                     /translation="MTFGARGITSLIACALCLWGSWAATVTAQEKYPSRPIRVIVPFA
                     PGGGSDVLARFLGPRLSERLGQPVVVDNRPAASGVVGADLVAKATPDGYTLLLVFSTH
                     AQSAQLFTRLPFDPIKDFAPVTLVITTPLGMMLNPAVPARTVKEFIAHAKANPGKLNY
                     GSSGPGSSPHLAAELFMSMAGVQMTHIPYKGVAPVITAQLGNEVQFSFANMFSTQGHW
                     KAGRLRLVAHAGAKRLEVMPDVPTIAESGVPGFEAIIWYGYMTAAKTPRPVVDRLNKE
                     IAAVANLSDVRQTLVSQGNEVIAGSPQDFAKLVKADAEKWGAIGRKIGVRLD"
     gene            2210..>2922
                     /locus_tag="AC16CX_07585"
     CDS             2210..>2922
                     /locus_tag="AC16CX_07585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3012407.1"
                     /translation="MKRLIFIHVGIACIAALAALPAAAQKADWPTKPVRFIVQFPPGG
                     SVDPLARLVGSKLSPVLGQQFIVDNRPGGSGSIGAGIAAKAVPDGYTYLFVFDTHAVN
                     PTLIPNLPFDTTKDLAPVMLIGTAPMAIATGASKPYKSFDDVIRAAKAKPDTVTYGSI
                     GNGSLGHLTMILVQQAAGIKLVHVPYKGGGPMTQDALGGQVELAIGSVAVLTPHVKGG
                     KLRAIAVTGDSRSHALPDVA"
BASE COUNT          491 a          923 c         1007 g          501 t
ORIGIN      
        1 attcccgcct cgcggatcac cttgccgagc cgggcgttgt tgtccttgat gacggcggcg
       61 aacgcgtccg gcgagccgcc gacaacatcg aagcccatcg cggtgagccg ctcgcgtatg
      121 tcgggcagct tgctgatgcg tgcgagttcg ccgtggagct gcgaaacgat cgcgggcggc
      181 gtgcccgtcg gcgcgatgaa gccggtccag gaatcgaact cgtagcccgg cacacccgct
      241 tgtgcgatgg tggggatctc cggagtgacg ctggtgcgcg tcgcacccgt ggtcgcgatg
      301 atgcgcaggc gcccggtctt gtggaatggc agggcggtga ccagcccggg gatgatcatg
      361 tgcatttcgc cggcgagcac gtcattgaaa gcgggggtga gccccttgta gggaacgtgc
      421 acgatgttga tgccggccag cgatttcagc atttccatcg gtacgtgctg cgcactgccg
      481 acgccgccgg aggcatagtt caactgcccc gggcgcgctt tcgccagcgc gaccagttcc
      541 ttcaccgatt tcgcggggag cgaggggtgc accaccagac agttgcggat gaccgccgcc
      601 tgcaggattg gcacgtaatc ccgcgtcacg tcgtacggca gcttctggta gaggtggggg
      661 ttcatcgcga gcgtcgcggc gttgcccatg ccgagcgtgt agccgtcggg cgccgcgcgc
      721 gcgatcgctt ccatgccgat gatgccggcg gcaccggtgc gattatccac cagcacctgc
      781 tgcccccagg tttccgccat cttctgcgcg tagagccgcg ccagcacgtc caccgcgctg
      841 gcggcggtga agggcagcac caggcggatg gttttcgcgg gatatttctg ggcgagcgcg
      901 cccgccggcg cggcgagcac cacggcgaga agcactgcag ctgcgaggcg aaggctcatg
      961 tcttgtcctc ttggacgagt aacaacgagt aatgcgtgga tggtcgattc acgactcacg
     1021 attcatgctt ctgatgaatc agtcgagcct gaccccgatc ttcctgccga tcgcccccca
     1081 tttttcggca tcggccttga ccagcttcgc aaaatcctgc ggcgagccgg cgatcacctc
     1141 gttgccctgg gagaccaacg tctggcgcac gtctgagagg ttggcgacgg cagcgatctc
     1201 cttgttgagc ctgtcgacga cgggccgcgg cgtcttggcg gcggtcatgt agccgtacca
     1261 gatgatcgcc tcgaagccag gcacgccgga ctcggcgatg gtgggcacgt cgggcatgac
     1321 ttccagccgc ttcgcgcccg cgtgggcgac gagccgcagc cggcccgcct tccaatggcc
     1381 ctgggtcgag aacatgttgg caaacgagaa ctgcacctcg ttgccgagct gcgccgtgat
     1441 gaccggggcg acgcctttgt acggaatgtg cgtcatctgc acgccggcca tggacatgaa
     1501 cagctccgcc gcgaggtgcg gcgagctgcc cggaccggac gagccgtagt tgagcttgcc
     1561 gggattggct ttcgcgtgcg cgatgaactc cttcaccgtc ctcgcgggca ccgccggatt
     1621 caacatcatg ccgagcggcg tcgtgatcac cagcgtgacc ggcgcgaaat ccttgatcgg
     1681 gtcgaacggc agcctggtga agagctgcgc gctttgcgcg tgggtcgaga atacgagcag
     1741 cagcgtgtag ccgtcgggcg ttgccttggc gacgaggtcc gcgccgacga cgccggaagc
     1801 ggcgggccgg ttgtccacca ccaccggctg cccgagccgt tcggagagcc gcgggccaag
     1861 gaagcgggcg aggacatcgc tgccgccgcc gggcgcgaac ggcacgatga cgcggatcgg
     1921 gcggctcggg tatttttcct gcgcggtgac tgtggctgcc cacgagcccc acaggcacaa
     1981 cgcgcatgca atcaggctgg tgattccccg cgcaccgaac gtcatggcct cctcccggtt
     2041 cgtgaatgga aaacgcgggg tcatattgca tcaaatggcg agcggtgacc agtgacgggg
     2101 gatccctggg gaagagcggc ggattgagag acgcgggaaa aaacgagtgg cgcagtgaca
     2161 gagcgacggg taactggtgt taacctatga ggagtgaagg aggcagacca tgaaacgcct
     2221 gattttcatt catgtcggga ttgcatgcat cgccgcgctg gcggcgctgc ccgccgccgc
     2281 gcagaaggcc gactggccga ccaaaccggt gcgcttcatc gtgcagttcc cgccgggggg
     2341 ctcggttgac ccgctggcgc gtctggtggg gagcaagctc tcgccggtgc tgggccagca
     2401 gttcatcgtg gacaaccgcc ccggcggctc gggttcaatc ggcgcgggta tcgccgcaaa
     2461 ggcggtgccg gacggctata cctatctctt cgtattcgat acgcacgctg tcaaccccac
     2521 gctgatcccg aacctgccgt tcgacaccac gaaggacctc gccccggtga tgctcatcgg
     2581 cacggcgccg atggcgatcg cgaccggtgc ttcgaagccg tacaagagct tcgatgacgt
     2641 gatccgggcg gcgaaggcga aacccgatac ggtcacctac ggctcgatcg gcaacggctc
     2701 gctcggccat ctcaccatga tcctggtgca gcaggccgca ggcatcaagc tggtgcacgt
     2761 gccctacaag ggcggcgggc cgatgacgca ggacgcgctg ggcggccagg tcgagctcgc
     2821 gatcggctcg gtcgcggtgc tcacgccgca tgtcaagggc ggaaagctgc gcgccattgc
     2881 ggtcacgggc gacagccgtt cgcacgcgct gccggacgtg gc
//
LOCUS       DBNGWZ010000253         4457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2545521, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000253 DBNGWZ010000000
VERSION     DBNGWZ010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2545521"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..470
                     /locus_tag="AC16CX_07590"
     CDS             231..470
                     /locus_tag="AC16CX_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012408.1"
                     /translation="MALNPDRIGRAFYDELRRHYSEEEIVELGAFIGFNIGYHTFFGT
                     LKFYPMFSPDGRLVTQEESQRIYGAVPASLAGVTK"
     gene            627..1199
                     /locus_tag="AC16CX_07595"
     CDS             627..1199
                     /locus_tag="AC16CX_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3012409.1"
                     /translation="MPHIEPIGLEEIRDPELRSLIARCEALGVPDSLFPRILARVPAH
                     AKALLRALLVSHAEGNVDHRLKEIIRVRLARTAGDPYFAGLRSAKAKREGVDEETIEA
                     GSGKFEDDPRFTAAEKWALRYAREMYLNPEKVDAAFYDEGKRHYTEAQIMELGAFIAF
                     HYGMQAFTRTLGAAPLRGAGDAVTQSRKKM"
     gene            1214..2458
                     /locus_tag="AC16CX_07600"
     CDS             1214..2458
                     /locus_tag="AC16CX_07600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006493921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3012410.1"
                     /translation="MAATERQLADSIPSAGVVARPVGKPPRILRRMLALDDFEEAARW
                     YIPRPIYGYVTGGAETNAALRGNRAVWDEIAFVPRVLLDVSGRTQKTTLFGRTYDAPF
                     GIAPMGGTAMAAYQGDLVLARAAAQANVPMILSGAALTPLERVREEGPTTWFQAYLPG
                     ETGSITALIERVARAGYDTLVLTVDVQVLANRENNVRSGFHTPLRPTLRLAWDGLVRP
                     RWLFGMLLRTLLLHGMPHFENMGPRVPLLSRTAERDRGRRDQLAWKHLELMRRLWKGR
                     LVVKGILDKDDARIARESGVDGIIVSNHGGRQLDGAAAPLRVLPAIAAEAGGMTVMMD
                     SGVRRGTDVLKALALGAQFVFLGRPFLYAAAIAGMPGMLHAIKLLRDEIDRDMALVGI
                     TTLAEMRRELLMPARGAEFFAR"
     gene            2705..3439
                     /locus_tag="AC16CX_07605"
     CDS             2705..3439
                     /locus_tag="AC16CX_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012411.1"
                     /translation="MTDASPAIRSQLAPTGKLRVGINMSNFLLTAKDPATGEPRGIAV
                     DLGRELGRRLGVPVEIVPYPNPGALADVATSGAWDVGFLGAEPQRANEIAFTAAYVEI
                     EATYLVPSGSPLKTIADVDRKGVRIAVPEKSAYELYLTRSLKNAELARTQGADNAFKL
                     FVSDKLDALAGLRPRLVKDAGNLPGSRILDGRFTAVQQAIGTPKARGAGAKFLREFVE
                     DIKATGLVARTIEKNGVRGLTVAPKA"
     gene            3445..4122
                     /locus_tag="AC16CX_07610"
     CDS             3445..4122
                     /locus_tag="AC16CX_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3012412.1"
                     /translation="MSEIKVMLSLAFKEAYLELVPGFERASGHKVTNLWVPSVQMMNR
                     LKSGETVDLVILSAALLDELIKAGLIADRADLAKSGVGVAVKAGAPKPDIRSGEAVKR
                     VVLAAKSIVYSTGPSGIYLAGLFQRMGIAERIKSKVRQVQGEPAGALVARGEAEIGFQ
                     QVSELLPVPGIDLVGPLPPDIQQITVFSAGVHVAAKQPDAARALVKFLRAPAAAPVIR
                     KKGMEPA"
BASE COUNT          816 a         1487 c         1459 g          695 t
ORIGIN      
        1 tcgcctggga cacgatcttc aacaccggcc gcgtcagcca cagactgaag gagatcatcc
       61 gggtgcagct ttcccgcatg gcctcctgcg tctactgagg aaacgtccgg tccgcttcgg
      121 cgaagcggca gggactgaag gaggacgaca tcgacgaggg catcgcccat tacgcggcga
      181 gcccccgctt caccgaggcc gagaagctcg cgctgcgcta cagcgagctg atggccctca
      241 acccggaccg gatcgggcgc gcgttctacg acgagctgcg ccggcactat tccgaggagg
      301 agattgtcga gctcggcgcc ttcatcggct tcaacatcgg ctatcacacc ttcttcggca
      361 ccctcaagtt ctacccgatg ttctcgcccg acgggcggct cgtcacccag gaggaatcgc
      421 aacgcattta cggtgccgta cccgcatcgc ttgccggagt gacgaaatga cccgcggtgg
      481 aaccgtgaga gcggaagagc gtgagggcgg gagggcgata actaccggga gggcgtgaga
      541 gcgggagagg gacaatcccg attcccgctt tttccatctc ccgatctccc gctctcccga
      601 gttttcggga cgactggaga tatctgatgc cgcacattga accgataggg ctggaggaaa
      661 ttcgggatcc tgagcttcgc tcgctgatcg cgcgctgcga agcgctcggc gtgccggact
      721 cactctttcc ccgcattctc gcgcgcgtgc ccgcgcacgc gaaggcgttg ttgcgggccc
      781 tgctcgtctc gcatgccgag ggcaacgtgg atcaccgcct gaaggagatc atccgcgtgc
      841 ggctcgcccg caccgcgggt gacccgtact tcgccggcct gcgctcggcg aaggcaaaga
      901 gggaaggcgt cgacgaagag acgatcgagg cgggttcggg caagttcgaa gacgacccgc
      961 gctttactgc agccgagaaa tgggcgctgc gctacgcacg cgagatgtat ctcaatcccg
     1021 agaaggtgga cgccgccttc tacgacgaag gcaagagaca ctacaccgag gcgcagatca
     1081 tggaactcgg cgcctttatc gccttccact acggaatgca ggcgttcacg cgcacgctcg
     1141 gggccgcgcc gttgcgcggc gccggtgacg cagtgacgca atcacgcaaa aaaatgtgac
     1201 aatgggcgac gatatggctg ctaccgaacg tcaactggct gattccatcc cttcggcagg
     1261 cgtggtcgcc cggccagtcg ggaagccgcc gcgcattctg cgccggatgc tcgctctgga
     1321 cgatttcgag gaggcggcgc gctggtacat accgcggccg atctacggct acgtcacggg
     1381 cggcgccgaa accaacgccg cgctgcgcgg gaaccgggcc gtttgggacg agatcgcgtt
     1441 cgtccccagg gtgctgctgg acgtgtccgg gcggacgcag aagaccaccc tcttcggccg
     1501 cacttacgac gcgcccttcg ggatcgcgcc gatgggcggg accgcgatgg ccgcctacca
     1561 gggcgacctc gtgctcgccc gcgcggcggc acaagccaac gtcccgatga tcctgagcgg
     1621 cgccgcgctg acccccctcg agcgggtcag ggaagaaggg ccgaccacgt ggttccaggc
     1681 ttatcttccc ggggagaccg gctcgatcac cgcgctcatc gagcgggtcg cgcgcgccgg
     1741 ctacgacacc ctggtcctca cggtggacgt gcaggtgctg gccaaccgcg aaaacaacgt
     1801 gcgcagcggc ttccacacgc cgctgcgccc cacgctgcgc ctcgcctggg acgggctcgt
     1861 ccgcccgcgc tggctcttcg gcatgctcct gcgcacgctg ctgctgcacg gcatgccgca
     1921 cttcgagaac atgggcccgc gcgttccgct gctttccagg acggcggaac gcgaccgggg
     1981 gcggcgcgac cagctcgcct ggaagcatct cgaattgatg cggcgcctgt ggaaggggcg
     2041 gctcgtggtg aaggggatac tcgacaagga cgacgcccgc atcgcccgcg agagcggcgt
     2101 ggacggcatc atcgtctcga accacggcgg gcgccagctc gacggcgcgg cggcgccgct
     2161 gcgcgtgctg ccggcaatcg ccgccgaggc cggcggcatg acggtgatga tggacagcgg
     2221 cgtgcggcgc ggcaccgacg tgctgaaggc gctcgcgctc ggcgcgcagt tcgtcttcct
     2281 cggccggccg ttcctctacg ccgccgcgat cgccggcatg cccggcatgc tgcacgcgat
     2341 caagctgctg cgcgacgaga tcgaccgcga catggcgctg gtcggcatca cgaccctcgc
     2401 cgagatgcgc cgcgagctgc tgatgcccgc ccgcggggcg gagttttttg caaggtgacg
     2461 aaaaagcttc accgccaagg acgcggaaga cgcaaaggaa aataacagcc tcaccgccaa
     2521 gtgcgcaagg acgcgaaggg aacaataatg ggaaccaaac cccaaggacg ccaagacaac
     2581 gccaagcgtg gtatctgggt gacgatcggg gatttggaac gccagtaacc ccctcactcc
     2641 tcacccctta ctcctcacag gctttgcccc tcactcctca caggcattta aataggtgac
     2701 cgcaatgacc gacgcctctc ctgccatccg ctcccagctc gcgccgaccg gcaagctgcg
     2761 cgtgggcatc aacatgagca acttcctgct caccgctaaa gaccccgcga cgggagaacc
     2821 tcgcggcatc gcggttgacc tggggcgtga actgggcagg cggctcggcg ttcccgtcga
     2881 gatcgttccc taccccaatc ccggggctct cgccgacgtg gcgacgagcg gtgcgtggga
     2941 cgtgggattc ctcggcgccg aaccgcagcg cgcgaacgaa atcgcgttca ccgcggccta
     3001 cgtcgagatc gaggccacct acctcgtgcc gtccgggtcg ccgttgaaga ccatcgccga
     3061 cgtggaccgc aagggcgtgc gtatcgcggt gccggagaag agcgcctatg agctgtacct
     3121 cacccgctcc ctgaagaacg cggagctcgc gcgcacgcag ggtgcggaca acgcattcaa
     3181 gctgttcgtc tcggacaagc tcgacgccct cgccggactc aggccgcggc tcgtgaagga
     3241 cgccgggaat cttcccggat cgcgcatact cgacgggcgg ttcaccgcgg tccagcaggc
     3301 catcggcacg cccaaagccc gcggcgcggg cgcgaagttc ctccgcgaat tcgtcgagga
     3361 catcaaagcc acggggctgg tggcgcgaac gatcgagaag aacggcgttc gcggcctcac
     3421 cgtcgcccca aaggcgtgac ctccatgtcc gaaatcaaag tcatgctgtc gctggccttc
     3481 aaggaggcct atctggagct cgtgcccggg ttcgagcgcg ccagcgggca caaggtaacg
     3541 aacctctggg tcccgagcgt gcagatgatg aaccggctca agagcggcga gacggtggac
     3601 ctcgtgatcc tgtccgccgc cttgctcgac gagctcatca aggccgggct catcgccgac
     3661 cgcgccgatc tcgcgaaatc gggcgtcggc gtcgccgtca aggcgggcgc gccgaagccg
     3721 gacatccgct cgggcgaggc cgtgaagcgc gtggtgctgg cggcgaagtc gatcgtctac
     3781 tccaccgggc ccagcggcat ttatctggcc ggactgttcc agcgcatggg catcgccgag
     3841 agaatcaaat cgaaggtcag gcaggtgcag ggcgagccgg ccggcgcgtt ggtggcgcgc
     3901 ggcgaggcgg agatcggctt tcagcaggtg agcgagctgc tccccgtccc cggcatcgat
     3961 ctcgtcgggc cgctgccgcc ggacatccag caaatcaccg ttttctcggc cggcgtgcat
     4021 gtggcggcga agcagccgga tgcggcgcgg gcgctggtca aattcctcag ggcgcccgcc
     4081 gcggcgcccg tcatcaggaa gaagggaatg gagcccgcct aatgagcgtc gtaagagtgc
     4141 ttgacgacgc tcatccctca cctccgcccc tctcccccag aggagagggg agattcgagg
     4201 tgctaatgcc ttgtcaggca ttcgcacgag gaccaataat gccggaacag cgtgaatcgt
     4261 gattcgcgag tcgtgaatcg attcttgccg ggcgacaaac gcgctgttga tagttgccgc
     4321 cagcagtgtc cgcgggtatt cttcgctggc ggctctgccg accccactac aaggagaacc
     4381 ccgcatggaa atcaaaacag gcgtcgcagc gcccgatttc acgctcgccg cgcacaacac
     4441 cgatcaaaaa atcgcgc
//
LOCUS       DBNGWZ010000254         3063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_780651, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000254 DBNGWZ010000000
VERSION     DBNGWZ010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_780651"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16CX_07615"
     CDS             complement(<1..257)
                     /locus_tag="AC16CX_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012413.1"
                     /translation="MNDLTQAFAAAFGLLARLDVKLIEIVALSLQVSLTAVVIASAVG
                     LPLGAALAVWRFHGRQPVIILLNALMGLPPVVVGLLVYLLLS"
     gene            434..1708
                     /gene="moeA"
                     /locus_tag="AC16CX_07620"
     CDS             434..1708
                     /gene="moeA"
                     /locus_tag="AC16CX_07620"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3012414.1"
                     /translation="MNDPVQPLRALSCADDYDPNSMPVEKAREVIARFLTPVTATERV
                     HVRAALGRVLAEDVTSPLNVPAHDNSAMDGYAVRFADLKSDGEVTLKVAGSSFAGAPY
                     KGSVGAGQCVRIMTGGVVPHGTDTIVMQEHVKAAGDKVTIGPGHRKGQNLRRAGEDLA
                     AGQVALKRGLALRPAEIGLISSLGIGEISVYRKLRVAFFSTGDELVSIGVQPREGQIY
                     DSNRYTIYGMLTRLGCEVSDMGVVRDDPALLEKAFQDAAAAADVVITSGGVSVGEADY
                     VKDLLNKLGEVVFWKIAMKPGRPLAYGKIGAAHFFGLPGNPVSVMVTFYQFVRDALLK
                     LAGRDPVPALPTFKVACTSSLKKAPGRTEFQRGVLSQDASGNWSVRVTGEQGSGILRS
                     MSEANCFIILPTDQGNVTPGSLVDVQVLEGVV"
     gene            1814..>3063
                     /locus_tag="AC16CX_07625"
     CDS             1814..>3063
                     /locus_tag="AC16CX_07625"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR3012415.1"
                     /translation="MATIKQEDFIQSIADGFQFISFYHPVDFIEAMGEAYEREQSPAA
                     KNAIAQILMNSRMAAEGHRPVCQDTGIAVVFLKVGMDVRWQATMSLQDMVNEGVRRAY
                     TDKDNPLRASILADPAGARKNTRDNTPAVVYTELVPGDKVEVKLAAKGGGSENKSRFA
                     ILNPSDSVVDWILEQVPTMGGDWCPPGMLGIGIGGTAEKAMVLAKESLLDPLDMHELK
                     ARGPRTRAEELRIEIFDKVNALGIGAQGLGGLTTVLDVKVRDYPTHATSLPVAVIPNC
                     AATRHLEFHLDGSGPAHFEAPGLEHWPRITVDLAAGAKRVNLETLTKAETATWKAGDR
                     LLLTGRIFTGRDAAHKRLADLIGKGEKLPVDFTNRLIYYVGPVDPVRDEVVGPAGPTT
                     SRRMDKFMDVMLGKTGLLGSIGKS"
BASE COUNT          563 a         1005 c         1004 g          491 t
ORIGIN      
        1 gacagcagga gatagaccag caggccgacc acgaccggcg gcagtcccat cagggcgttg
       61 aggagaatga tgaccggctg gcggccgtgg aagcgccaga cggcaagcgc cgcccccagg
      121 ggcagcccga ccgccgaggc gatcaccacc gcggtgaggc tcacctgcag gctcaaggcg
      181 acgatttcga tcagcttgac gtcgagccgc gcgagcaagc cgaacgctgc ggcaaatgcc
      241 tgggtgagat cgttcatcgt tccagagatc cgggtggcaa gacgggattt taagagaaat
      301 ccgcatcttg ccgctgtatc ccggctgccc tcgcctgctg atggtgtttt tcggttcgcc
      361 ggcacgtgct tcggccatcg tgcgaatcct gtaaaatttc ggcttaccgt tagcgtttac
      421 ccggcccctg ctgatgaacg acccggtcca acccctgcgc gccctgagtt gcgccgacga
      481 ctacgatccg aactcaatgc cggttgagaa agcgcgcgag gtgatcgcgc gtttcctgac
      541 gccggtcact gcgactgaac gggtgcacgt tcgcgccgcg ctgggccggg tgctggccga
      601 ggacgtgacc tccccgctca acgttcccgc ccacgacaat tccgcgatgg acggctacgc
      661 ggtgcgtttc gccgatctca agtccgatgg ggaagtcacg ctgaaggtcg ccggcagctc
      721 gttcgccggt gcgccataca aggggtcggt gggagccggg cagtgcgtgc gcatcatgac
      781 cgggggcgtg gtcccgcacg gcaccgacac catcgtgatg caggagcacg tcaaggccgc
      841 cggcgacaag gtcacgatcg gccccgggca caggaaggga cagaatctgc gccgtgccgg
      901 tgaggacctc gccgcgggac aggtcgcgtt gaaacgcggc ctggcgctgc gccccgccga
      961 gatcgggctc atctcctcat tgggcatcgg cgaaatcagc gtctaccgca agttgcgggt
     1021 cgcgttcttt tccaccggcg acgagctggt ctcgatcggc gtgcagccgc gggaaggcca
     1081 gatctacgac agcaaccgtt acacgatcta cgggatgctg acgcggctcg gctgcgaggt
     1141 cagcgacatg ggcgtagtgc gcgatgaccc cgcactgctc gaaaaagcgt tccaggacgc
     1201 ggcagcagcg gcagacgtcg tcatcaccag cggcggggtg tcagtcggtg aggccgacta
     1261 cgtgaaggac ctgctcaaca agctcggcga ggtggtgttc tggaagatcg cgatgaagcc
     1321 cggccggccc ctcgcctacg gcaagatcgg tgccgctcat ttcttcgggc tccccggtaa
     1381 cccggtgtcg gtgatggtca cgttctacca gttcgtgcgc gacgcgttgc tcaagctcgc
     1441 cgggcgtgac ccggtgcctg cactgcccac cttcaaagta gcatgcacct ccagcctcaa
     1501 gaaggcgccg gggcgcaccg agttccagcg cggcgtcctg tcgcaggacg cctcgggcaa
     1561 ctggagcgtg cgcgtcaccg gcgagcaggg ttccggcatc ctgcgctcca tgtccgaagc
     1621 gaactgcttc atcatactgc ctaccgacca ggggaacgtc accccgggca gcttggtcga
     1681 tgtgcaggtg ctcgaaggcg tagtctgacg gggcggtaag cggtaagcgg taaatcgggc
     1741 ccgcgttatt ttcgaagttc cccgctcaca gctcacagct tacggctcac catcttgatc
     1801 cgaaggatca ttcatggcca ccatcaagca ggaagatttc atccagagta tcgccgacgg
     1861 ctttcagttc atctctttct atcatccggt ggatttcatc gaggcgatgg gcgaagccta
     1921 cgagcgcgag cagtcccccg ccgcgaagaa cgcgatcgcg cagatcctca tgaactcgcg
     1981 catggccgcg gaggggcacc ggccggtgtg ccaggacacg ggcattgccg tggtgttcct
     2041 caaggtcgga atggacgtgc gctggcaggc caccatgagc cttcaggaca tggtcaatga
     2101 gggcgtgcgc cgcgcctaca ccgacaagga caacccgttg cgggcctcga tcctcgccga
     2161 tccggcgggt gcgcggaaaa acacgcgcga caacacgccg gcggtggtgt acacggaact
     2221 cgttcccggc gacaaggtcg aggtcaagct cgcggccaag ggcggcggct ccgagaacaa
     2281 gtcgcgcttc gccatcctga accccagcga cagcgtggtg gactggatcc tcgagcaggt
     2341 cccgaccatg ggcggcgact ggtgcccgcc cgggatgctc gggatcggca tcggcggcac
     2401 cgcggagaag gcaatggtgc tcgccaagga gtcactgctc gaccccctgg acatgcacga
     2461 gctcaaggcg cgcgggccga ggacgcgggc cgaagagctg cgcatcgaga tcttcgacaa
     2521 ggtcaacgcg ctcggcatcg gcgcgcaggg actcggcggg ctcaccaccg tgctcgacgt
     2581 caaggtacgc gactatccca cgcacgccac ctccctcccg gtcgcggtga tcccgaattg
     2641 cgccgcgacc cgccacctcg agttccacct cgacggcagc ggccccgcgc acttcgaggc
     2701 acccggcctc gagcactggc cccggatcac ggtcgatctg gcggccggcg cgaagcgcgt
     2761 caatctcgag accctgacca aggcggaaac cgcaacctgg aaggcgggcg acaggctcct
     2821 gctgaccggc aggatcttca ccggccgcga cgccgcccac aagcggctcg ctgacctgat
     2881 cggcaagggc gagaagctcc cggtggactt caccaaccgc ctcatctatt acgtcgggcc
     2941 ggtcgatccg gtgcgtgacg aagtggtcgg ccccgccggc cccaccacct cgcgccggat
     3001 ggacaaattc atggacgtga tgctcggcaa gacgggattg ctcggctcga tcggcaagtc
     3061 gga
//
LOCUS       DBNGWZ010000255         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1537418, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000255 DBNGWZ010000000
VERSION     DBNGWZ010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1537418"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16CX_07630"
     CDS             <1..230
                     /locus_tag="AC16CX_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012416.1"
                     /translation="AKRMTNLTFPVQYTVSQADVFLEGVTLGGKVNVFAKLDKDGNAG
                     PPEPGDMEGEHKGNPVMVGQTGVDITIDKIH"
     gene            579..1922
                     /locus_tag="AC16CX_07635"
     CDS             579..1922
                     /locus_tag="AC16CX_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012417.1"
                     /translation="MTKRIVMLGVLVGALLGATSSSWAVDLKWGGDYRLRGFYIDNLT
                     DADKNTQDSAAYYSSRFLLTLAATEDNVSGVVTLAAGTEGTNGNRLLGATSFGPDGSA
                     VGLVEAYLKADFKTWGLKGGRAIYKLGNGVILDDAVDGLWANFGLGATALTVATLKLN
                     EQTDTGSGVAIGNTAAPGTAGDADLYVINAGFGKMAMGPAHDLNLFAAYLKDRSATLV
                     GGGAIPDDVTLLILGASADADLGGLRLKGEFDFMDGTNKRPLLTDRDIKGMNLVLGAK
                     LNAGAIPLGVDFIYTTGLDPAKAGTEFNINGLSGNYPVGIIITNTGAVSDTYKDGTCL
                     SIGGGRAYAGGALNCLNGDGLTAVKVSTGMVHGPHTVDVAAIYAKSTEEPVALNGDTD
                     IGIELDATLTWALTKRLSAMGGIGYLISGDYWKFGVAAAPTDNMTVLVTQLAYTF"
     gene            complement(2047..2646)
                     /locus_tag="AC16CX_07640"
     CDS             complement(2047..2646)
                     /locus_tag="AC16CX_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HIR3012418.1"
                     /translation="MEKTFDDVVDFHRHLCLDIAMGYRVGKALVREMGKELRNMKDVF
                     AIVENETCAVDAIQEFTGCTLGKRNLILAGTGKPGYILQNAKSGAAVRIYCKYWETFD
                     ADGSFGKRRKHASSPDATTEEQQLFQRDLNTKIKEILRAPEETLFTIQRVSIPPPPKS
                     GKYKAEPCAGCGEHTNVERLTVVDGKKLCRACQPAAVGG"
BASE COUNT          507 a          907 c          995 g          534 t
ORIGIN      
        1 ccgccaagcg gatgacgaat ctgaccttcc cggttcaata cacggtcagc caggccgatg
       61 tcttcctcga aggggtgacg ctgggcggca aggtcaacgt gttcgccaag ctcgacaagg
      121 acggcaatgc cggccctccc gagcccgggg acatggaagg cgagcacaag ggcaatcccg
      181 tgatggtcgg ccagaccggt gtcgatatca ccatcgacaa gatccattga cgagtctctt
      241 gagcccggcg gtgcgccggg cggggcgtgg tcgtgcctgc gctgcgacgc gtgcgggggg
      301 ccgctgctcg acctccattg caagctggtg tgccgccggt gcgggtttat gcgcgactgc
      361 tccgatccgt gatcggcatt gcgcgcggcg tctgctcttt acaaatatcc aataagtcgg
      421 tatcttttcg cggcatcggt cgcgatgatg gcacggcggc gcgcggcggc gccgctcgca
      481 gggaggaggg cgccggtcgg ttccgcgcca tcggcgcggg ggtccctaag tgatgaggga
      541 taggtcatca caccatctct tcaggtagga ggagaacgat gacgaagcga atcgtaatgc
      601 tcggggtgct ggttggcgcc ctcctggggg cgacctcgtc ctcgtgggcg gtcgacctca
      661 aatggggcgg ggattaccgc ctgcgcgggt tttacatcga caacctgacg gatgcggaca
      721 agaacaccca ggacagcgcc gcgtactact cgtcccggtt tctgctgacc ctggcggcga
      781 ccgaggacaa cgtgagcggc gtggtgacgt tggccgcggg gacggagggg accaacggca
      841 atcgcctcct cggtgcgacg tcgttcggcc cggacgggag cgcggtcggc ctggtggaag
      901 cctacctcaa ggcggatttc aagacgtggg ggctcaaggg cgggcgcgcg atctacaaac
      961 tgggcaacgg cgtcatcctg gatgacgccg tggacggcct ctgggcgaac ttcggcctcg
     1021 gcgcgaccgc cctgacggtc gcgaccctca aactgaacga gcagaccgac accggctcgg
     1081 gggtggcgat cggcaacacc gctgcgcctg gaaccgcggg cgacgccgat ctgtatgtga
     1141 tcaacgcggg gttcggcaag atggcgatgg ggccggcgca tgacctcaac ctctttgccg
     1201 catacttgaa ggaccggtcg gcgaccctgg tcggcggtgg tgccatacct gacgacgtga
     1261 cgctgttaat cctcggcgcg tcggccgacg cggatctggg agggcttcgc ctcaagggcg
     1321 agttcgattt catggacggc acgaacaaga ggcccctcct caccgacagg gacatcaagg
     1381 gcatgaacct ggtcctgggc gccaaattga acgcgggcgc gataccgctg ggggttgact
     1441 tcatctacac gacgggtctg gatcccgcga aggccggtac cgagttcaat atcaacggcc
     1501 ttagcggcaa ctacccggtc gggatcatca tcaccaacac cggcgcggtg tcggatacgt
     1561 acaaggacgg aacctgcctg agcatcggcg gcgggcgtgc gtatgccggc ggcgcgctga
     1621 attgtctcaa tggcgacggc ctgaccgcgg tgaaggtgtc gacggggatg gtgcacggac
     1681 cgcacacggt cgatgtcgcc gcgatctacg ccaagtcaac cgaggaaccg gtggcgctga
     1741 acggcgacac ggacatcggc atcgagctcg acgccacgct gacctgggcg ctgaccaaga
     1801 ggttgagcgc gatgggcggg atcggctacc tgatctcggg cgattactgg aagttcggcg
     1861 tggccgctgc gccgaccgac aacatgacgg tgttggtcac ccagctcgcg tatacgttct
     1921 aacgagcttc accggttgaa acagaaagag gggacagccg ctggccgtcc cctcttcgtt
     1981 tattatccgt tctcatccac cccccctttt gtcaaagggg gggcaagggg ggattttcta
     2041 atcggctcag cccccgacgg ccgctggttg gcacgcgcgg cagagcttct tcccatcgac
     2101 cacggtgagc cgttccacgt tggtgtgttc cccgcagccc gcgcacggct cggccttgta
     2161 cttgcccgac ttcggcggcg gcgggatcga cacgcgctgg atcgtgaaca gggtctcttc
     2221 cggggcgcgc agaatctcct tgatcttggt gttgaggtcg cgctggaaga gctgctgctc
     2281 ttcggtcgtg gcgtcggggg aggaggcgtg cttgcgccgc ttgccgaacg agccgtccgc
     2341 gtcgaaggtc tcccaatact tgcagtagat ccgcaccgcc gcgccggact tggcgttctg
     2401 gaggatgtat cccggcttgc cggtgccggc gaggatcagg tttcgcttcc ccagcgtgca
     2461 ccccgtgaac tcctggatcg catcgacggc gcacgtctcg ttctccacga ttgcgaacac
     2521 gtccttcatg ttccggagtt ccttgcccat ctcccgcacg agggcctttc ccacccggta
     2581 tcccatcgcg atgtccaggc acaggtgccg gtgaaagtcc accacgtcgt cgaacgtttt
     2641 ttccatacat cctcgcttgc gtagcgcaac gtagggcccg aggctgcgag aaggtgttga
     2701 ccaacacgtc tttcccgcta ccgtggcgcc agtataggcg aaagcggcgc gcgaacgcaa
     2761 tcgcggggcc tgattgatcc acgctccggc gcctgctatg atccggcccg atgtacatcg
     2821 aggttgaggc gaatcccaat tgcgcggggc gcgaagggat gcggtaccgg gagttgggcc
     2881 cgccgtcgcc catcatccgg gtccggatct tcgaccggcg cgacgagggc gagtggtgcc
     2941 tcg
//
LOCUS       DBNGWZ010000256         3117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1789622, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000256 DBNGWZ010000000
VERSION     DBNGWZ010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1789622"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16CX_07645"
     CDS             <1..629
                     /locus_tag="AC16CX_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR3012419.1"
                     /translation="FEFECSSLKRFDPDDPERPPIVKYLPSWEGPQAKELYERYPLQL
                     ISPHSRFSYHTLSDGKDSSINDIQEHRVLVDGYYYWVLRIAGEDARERGIKEHDLVKI
                     YNDRGGVVCAAHLTERLPRGVVHAYESSACYEPLGEPGYSVDRGGCINQLTPKRSIAK
                     KVTGTAANSCLVQVMPWDGSKGLAAGAATGAQVAAQKAAAAQVPAMAK"
     gene            637..1500
                     /locus_tag="AC16CX_07650"
     CDS             637..1500
                     /locus_tag="AC16CX_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3012420.1"
                     /translation="MKKWNLIIDVARCFGCNACAVACHDEYYGNEFPGYAAEMSKHGQ
                     RWIDILQREKGRFPMVEVAYLPVTCNHCDDAPCIKAAKDGAISKRPDGIVIIDPVKAK
                     GQRQLVDACPYGAIHWNEEKQLPQAWPFDAHLLDRGWTRTRGSQVCPTQAIRTVHVDD
                     EEMQRIAREEKLEVLHPEYHTKPRVYYKNLDRFFKAFVGGSLAGDIDGVTECVEGARV
                     RLQHGGRTVAETTSDAYGDFRFQGLEENSGAYRIEVADARFDGKVVEFDLRESTYLGT
                     ISLERGAAVQA"
     gene            1634..>3117
                     /locus_tag="AC16CX_07655"
     CDS             1634..>3117
                     /locus_tag="AC16CX_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3012421.1"
                     /translation="MNKPDELSKVHAKIEAILPRHRDLYYDGKWQKPKGGYQDTLNPA
                     TGQSLGPCAEANAQDVDAAVQAAHQAFKEWRRMKPLERAALMKKVAGALRSNAEELAM
                     LDAANCGNPVREMMRDAGTAAAQWDFFAGLVTEIKGETIPMGDGVVNLSVREPFGVCA
                     RIVAYNHPLMFTAAKAAPAIAAGNTVIMKPPYQAPLPAYRFMELVDGILPPGVLNIIT
                     CGKEGAEALVAHPLVPRVSLIGSVPTGRAIMKAAADRLKHVSLELGGKNACIIYPDAD
                     LEKAAKGAVGGMNFAWCGQSCGSTSRLFVHESVYDRVMEGVMKQIKVHKPGIPTEMDT
                     TMGAIVSRAQMEKVLGYIEIGKKEGATLAYGGKRPDDPKLAGGFFVEPTVFTNVTQKM
                     RIANEEIFGPVLSVLKWKDEDALFEQVNAVEYGLTGSVWTTNLANAHRAASRIESGFI
                     WVNGAGPHFLGVAFGGYKQSGIGREESYEELLSFTQSKNINITL"
BASE COUNT          611 a          981 c         1061 g          464 t
ORIGIN      
        1 agttcgagtt cgagtgctcg agcctcaagc gcttcgaccc ggacgatccc gagcgtcccc
       61 cgatcgtgaa gtacctgccg tcctgggaag ggccgcaagc gaaagagctc tacgagcgct
      121 atccgctcca gctcatctcg ccgcactcac gcttctccta tcacacgctc tccgacggca
      181 aggacagctc gatcaacgac atccaggaac accgcgtgct cgtggacggc tattactact
      241 gggtgttgcg catcgccggc gaggacgccc gggagcgcgg catcaaggag cacgacctgg
      301 tgaagatcta caacgatcgc ggcggggtcg tctgcgccgc gcacctcacc gagcggctgc
      361 cgcggggcgt ggtgcacgcg tacgagtcct cggcctgcta cgagccgctg ggcgagcccg
      421 gctactcggt ggatcgcggg gggtgcatca atcagctgac accgaagcgc tcaattgcga
      481 agaaggttac cggtaccgcg gccaattcct gcctcgtgca ggtgatgccg tgggatggat
      541 caaagggcct cgccgccggc gcggcgaccg gcgcgcaggt tgcggcccag aaggccgccg
      601 cggctcaggt gccggcgatg gcgaagtgag gcacgcatga aaaagtggaa cctcatcatc
      661 gatgtcgcca ggtgcttcgg ctgcaacgcg tgcgcagtcg cctgccacga cgagtactac
      721 ggcaacgagt ttccggggta cgcggcggag atgtcaaagc acgggcagcg gtggatcgac
      781 atcctccagc gcgagaaggg ccggtttccg atggtggagg tcgcctacct gccggtgacc
      841 tgcaatcact gcgacgatgc gccgtgcatc aaagccgcga aggacggcgc gatcagcaag
      901 cgaccggacg gcatcgtcat cattgatccg gtcaaggcga aaggccagcg gcaactggtg
      961 gacgcctgcc cctacggcgc catccactgg aacgaggaga aacagcttcc gcaggcgtgg
     1021 ccgttcgacg ctcacctgct tgatcgaggc tggaccagga cgcgcggttc tcaggtctgc
     1081 ccgacgcagg cgatacgcac ggtgcacgtg gacgacgagg agatgcagag gatcgcgcgc
     1141 gaggagaagc tggaggtgct ccatccggag taccacacga agccgcgggt ctattacaag
     1201 aacctcgacc ggttcttcaa ggccttcgtc ggaggaagcc tcgccggcga catcgacggc
     1261 gtcaccgaat gcgtcgaagg cgcgcgcgtg cgactgcagc atggcggccg gacggtcgcc
     1321 gaaacgacga gcgatgcgta cggcgatttc aggttccagg gactggagga gaacagcggc
     1381 gcttaccgca tcgaggtcgc cgacgcgcgg ttcgacggca aggtggtcga gttcgacctc
     1441 agggaaagca cgtacctggg cacgatttcg ctggaaaggg gagccgcagt ccaggcttag
     1501 gttcgagtgc gacgggtcat gccgggctgg cacttcccgc actggcgccc cgttttgccg
     1561 gcgagtccac tcgacgcacg cgcacagccc ggctacgatg ccggctcgac gcaaccctgg
     1621 agaccagcct cacatgaata aacccgacga gctttcgaag gtgcacgcca agatcgaagc
     1681 gatcctgccc aggcaccgcg acctctatta cgacggaaaa tggcagaagc cgaagggggg
     1741 ttatcaggac acgctcaacc cggcgacggg acaaagcctg gggccctgcg ccgaggccaa
     1801 cgcgcaagac gtcgatgccg cggtccaggc ggcgcaccag gcgttcaagg aatggcgccg
     1861 gatgaagccg ctcgagcgcg cggcgctgat gaagaaagtc gcgggggcgc tgcgttccaa
     1921 cgccgaagag ctcgcgatgc tggacgccgc caactgcggc aacccggtgc gcgagatgat
     1981 gcgggacgcc ggcacggcgg ccgcgcagtg ggatttcttc gcagggctcg tcaccgaaat
     2041 caaaggcgag accatcccga tgggcgacgg cgtggtgaac ctgtcggtgc gtgagccgtt
     2101 cggcgtgtgc gcgcgcatcg tcgcctacaa ccaccccctc atgttcaccg cggccaaggc
     2161 ggcaccggcc atcgccgccg gcaataccgt catcatgaag ccgccctacc aggcgccgct
     2221 cccggcctac cgcttcatgg agctcgtcga cggcatcctg ccgccggggg tgctcaacat
     2281 catcacctgc ggcaaggagg gtgcggaggc gctggtcgcg caccccttgg tgccgcgggt
     2341 gtcgctgatc gggagcgtgc ccaccggccg ggcgatcatg aaggcggcgg ccgatcgcct
     2401 caagcacgtg tcgctggagc tgggcggcaa gaacgcctgc atcatctacc cggacgcgga
     2461 cctggaaaag gcggcgaaag gcgcggtggg cggcatgaac ttcgcctggt gcggccagtc
     2521 gtgcggctcg acctcgcggc tgttcgtgca cgaatcggtg tacgaccggg tgatggaagg
     2581 cgtgatgaag cagatcaagg ttcacaagcc cggcatcccc accgaaatgg acaccaccat
     2641 gggggcgatc gtgtcccggg cgcagatgga gaaggttctc ggctacatcg agatcggcaa
     2701 gaaggaaggg gcgacgctcg cttacggcgg caagcggccg gatgatccga agctcgccgg
     2761 cgggttcttc gtcgagccga ccgtgttcac gaacgtcacc cagaagatgc gcatcgcgaa
     2821 cgaggagatc ttcgggccag tgctgtcggt gctcaagtgg aaggacgagg acgccctgtt
     2881 cgagcaggtc aacgcggtcg agtacggcct caccggctcg gtgtggacga cgaacctcgc
     2941 caacgcgcac cgcgccgcaa gccgcatcga gtcgggattc atctgggtga acggcgccgg
     3001 cccgcatttc ctcggcgtag cgttcggcgg ctacaagcag tcgggcatcg gccgcgagga
     3061 aagctacgag gaactgctct cgttcacgca atcgaagaac atcaatatca cgctcta
//
LOCUS       DBNGWZ010000257         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1412818, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000257 DBNGWZ010000000
VERSION     DBNGWZ010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1412818"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..1350)
                     /locus_tag="AC16CX_07660"
     CDS             complement(484..1350)
                     /locus_tag="AC16CX_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR3012422.1"
                     /translation="MKKTDIKSVVKERYAQAALSVATGAKASCCGRTPGKAQSSDPIT
                     SNLYEAAQTGELPQAAVLASLGCGNPTALAELKPGDVVLDLGSGGGIDVLLSAKRVGP
                     TGKAYGLDMTDEMLALANENKRKAGAQNVEFLKGEIEDIPLPDNSVDVIISNCVINLS
                     ADKDRVLREAFRVLKPGGRFAVSDVVTRGAVPSEIRNSMLLWVGCVAGALDENEYRAK
                     LAAVGFQNIDIEPTRIYRAEDAKGFLEDKGIDVSVIGPRVDGKFMSAFIRGTKPAAGA
                     TELETRARSTCC"
     gene            complement(1369..>2193)
                     /gene="arsB"
                     /locus_tag="AC16CX_07665"
     CDS             complement(1369..>2193)
                     /gene="arsB"
                     /locus_tag="AC16CX_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR3012423.1"
                     /translation="EVRRHWRGMAVTVAVNWAVKPFSMALLGWIFIRHVFAPWLPPEH
                     LDSYVAGLILLAAAPCTAMVFVWSQLAKGNPYFALSQVALNDTIMIFAFAPVVGLLLG
                     LSSIVVPWDTLFTSVLLYIVMPVLMAQMWRGLLMLRGGEARLHAVLAHLQPYSIVALL
                     ATIVLLFAFQGRQILTQPLIIALLAVPIVIQVYFNSALAYWLNRRLRVVHSVAGPSAL
                     IGASNFFELAVATAITLFGFDSGAALATVVGVLVEVPVMLSVVRIVNATRGWYERG"
BASE COUNT          441 a          691 c          627 g          434 t
ORIGIN      
        1 gaggccaggt tgccgttcat cttccagtta cctgctacca gcttgacgcg catccccggc
       61 ctggtcatcg taaaccgttg aattttacag gctagcgccg gccgtgcaaa gcatctgaat
      121 atcctcgaat taccggggtt gcccttattg ctagacatgg aaccgggcgt ccagtttcca
      181 cgtccggcgc aacaaaaaac gcccggccta tgccgtgcgt tttttgaatg ggaaagaaat
      241 gtcaattcgt ggggctggcg gggcagctct tacactcgag ttctgtattc cccactggcg
      301 atatggtcag ttccgcctcg gtttcgacct ggatgccaac gtggtcgagg accatttcat
      361 tgtcgctacg accatcaccc tgtcctttgc ggaacgcagt ctgatctgac actcggcacg
      421 ccggagacct gctcgcaaga aaacgaggtg attttccgct cgtgcttccc ttgcgcgcct
      481 gtgtcagcag catgtgctcc tagctctggt ttcgagttcc gtcgcgccgg cggctggctt
      541 tgttccgcgg atgaaggcgc tcatgaattt accatcaacc cgggggccga tcacgcttac
      601 atcgattcct ttatcctcga gaaacccctt agcgtcctca gcgcggtaga tccgcgtcgg
      661 ctcaatgtcg atgttctgga agccgaccgc tgcaagcttt gcgcggtatt cgttttcgtc
      721 cagcgctccc gccacacatc ctacccaaag cagcatgctg ttgcggattt ccgagggcac
      781 cgcacccctc gtcaccacgt cagacacggc gaagcgaccg cccggcttga gaacgcggaa
      841 cgcttcgcgc agcacgcggt ccttgtctgc cgagaggtta atcacgcagt tcgaaatgat
      901 cacgtctacc gagttgtcgg gcagcgggat gtcctcgatc tcacccttca gaaactcgac
      961 attctgcgcc ccggctttac gcttgttttc gtttgcgagc gccagcatct catcggtcat
     1021 gtcgagcccg taagcctttc cggttggacc cacacgcttc gcggaaagga gcacgtcgat
     1081 accgcccccc gacccgagat ccagcaccac atctcccggc ttcagttctg ccagcgccgt
     1141 ggggtttccg cagccgagcg aagccagcac tgcggcttgg ggcagctcgc cggtctgcgc
     1201 cgcctcatac aggttgctcg taatcggatc actggattga gccttgccag gcgttctacc
     1261 gcagcaggac gctttcgcgc cagttgcaac gctcaatgcc gcctgcgcat atcgctcttt
     1321 gacgacactc ttaatgtcgg tctttttcat ggcttctcct atgagaaatt agccgcgctc
     1381 gtaccagccg cgcgtagcgt tcacgatccg gaccaccgag agcatcactg gcacctccac
     1441 gagaacgccg acaaccgtgg cgagggcggc tccggaatcg aagccgaaga gcgtgatggc
     1501 ggtcgccacg gccaactcga agaaattgct cgccccgatg agcgctgaag ggcccgcgac
     1561 gctgtgtacg acacggagcc gccggttgag ccagtaggcg agcgcggagt tgaagtaaac
     1621 ctggatcacg atcggcacgg cgagcagtgc gatgatcagc ggctgggtga gaatctgccg
     1681 gccctggaac gcgaataaca gcactatcgt agcgagcaac gcaacgatcg aataaggctg
     1741 aagatgggcg agcacagcgt gaagtcgtgc ttctcctccg cgcagcatta gcaggcctcg
     1801 ccacatctgc gccatgagaa ccggcatcac gatatagagc aacaccgagg tgaagagcgt
     1861 gtcccagggc acgacgatcg aggacagccc gagcagcaag ccgacgaccg gcgcgaaggc
     1921 gaagatcatg atcgtgtcgt tgagcgctac ttggctgagc gcgaagtacg gattgccttt
     1981 cgcgagctgg ctccacacga acaccatggc ggtgcaaggc gcggccgcga gcaggatcag
     2041 gccggcgaca tagctgtcga ggtgctccgg cggcagccaa ggcgcgaaca cgtgacggat
     2101 gaagatccag ccgagtagcg ccatcgaaaa aggtttcacc gcccagttca ccgcgaccgt
     2161 caccgccatt ccgcgccagt gccgcctcac ctc
//
LOCUS       DBNGWZ010000258         7650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_655892, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000258 DBNGWZ010000000
VERSION     DBNGWZ010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_655892"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="AC16CX_07670"
     CDS             <1..894
                     /locus_tag="AC16CX_07670"
                     /inference="COORDINATES: protein motif:HMM:NF005792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HIR3012424.1"
                     /translation="LVLEQMWPLRESNRVHYGFNRYADFLEIQFNGGEHRHGVIAWML
                     AVLPAIALTAAVYYVLHGLSPLAGLLWSVVVLYLTMGFRQFSHYFTEIQQALRAGDLA
                     PARERLGKWRGEPASEFNASEIARVAIEQGLLASHRHVFGTIAWFVGLGPAGAVFYRA
                     SAVLADKWATRADPESGEFGRFAGRAFFWLDWVPARLTAASFAIVGDFEDSVYCWRTQ
                     AAAWATQAHGIILAAGGGALGVRLGDALHQYGSLQFRPELGVGDEAEADHMQGAAGLI
                     WRALVLWMFLIFLVSLAHALG"
     gene            complement(963..1832)
                     /gene="rsgA"
                     /locus_tag="AC16CX_07675"
     CDS             complement(963..1832)
                     /gene="rsgA"
                     /locus_tag="AC16CX_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3012425.1"
                     /translation="MHLLNGRIVASFGRRYLVELADASVLDCVTRGRRSALACGDRVS
                     VARSGRGQGVIEVLAPRSTLLYRSDRARQKLVAANVTQIVVVVAPVPTFYEDLLNRCL
                     AAAEHGCMAALIVLNKSDLPQAPAALGALDPYRRLGYRVLALSARQDLKPLAPHLKGQ
                     TSVLVGQSGMGKSTIINALVPGASVRVAEVSAALDSGRHTTTHARLYHLDAASQLIDS
                     PGMQEFGLHHLDPDELAHAFVEFRPFLGQCRFRDCRHVTEPGCAIAAACERGEINERR
                     LESYRGLVRERKP"
     gene            complement(1896..2237)
                     /locus_tag="AC16CX_07680"
     CDS             complement(1896..2237)
                     /locus_tag="AC16CX_07680"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3012426.1"
                     /translation="MTQIISDLASKKCKPCEGGVAPLKEQEIGNLLKQLAGWEYSNGR
                     IAKTYQFKNYYQTMAFVNATAWISHREDHHPDITVGYNQCRVEYVTHAIGGLSENDFI
                     CAAKLDKLFDM"
     gene            complement(2342..3580)
                     /locus_tag="AC16CX_07685"
     CDS             complement(2342..3580)
                     /locus_tag="AC16CX_07685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR3012427.1"
                     /translation="MNTFGIVFLAALAIATATRLWLARRHLSHVQAHRARVPDEFTAE
                     ISLAAHQKAADYTCAKTRFGMVGVAADVAVPLALTFGGVLQWLHGVSAAWLPDGIARG
                     LLLLVLVGALMTLVDVPFGLYRAFVIESRFGFNKLTPGLYFLDLAKSVALALLFGLPL
                     GAIVLWLMAAAGEYWWIYAWLAWVAFNLFVVAIYPTWIAPLFNKFSPMQDAALKERIE
                     RLLARCGFKVKGLMVMDGSRRSTHGNAYFTGFGASKRIVFFDTLLARLDPGEVEAVLA
                     HELGHFRLRHVAKRLAWLGLASLAFLWLLGWIMQQGWFYAGLNVQTPSAAMALVLFSI
                     VVPQFTVLLQPLGAMYSRKHEYEADRYAAQNASARDLVSALIKLYRDNASTLTPDPLH
                     SAFYDSHPPAIARIARLQKV"
     gene            3762..4307
                     /gene="orn"
                     /locus_tag="AC16CX_07690"
     CDS             3762..4307
                     /gene="orn"
                     /locus_tag="AC16CX_07690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR3012428.1"
                     /translation="MAQDPNNLIWIDMEMSGLDPDNDRILEVAMVVTDSRLDTVAEAP
                     VLVVHQPDAVLDRMDDWNKSTHAKSGLIERVKASTLGVADVEALMITFLAGHVPPKIS
                     PMCGNSVHQDRRFLARHMPGLEAYFLYRNLDVSTLKELAKRWKPEIMAGLVKQGKHEA
                     LADIYESIEELKYYREHFLKL"
     gene            complement(4408..4614)
                     /locus_tag="AC16CX_07695"
     CDS             complement(4408..4614)
                     /locus_tag="AC16CX_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HIR3012429.1"
                     /translation="MNDEALNMSIRKFLKTVGVNSQLAIEKAIHKAIADGKLKGNEAF
                     PAAMTLSIGKLGLDVKFDGEIRLE"
     gene            complement(4703..6355)
                     /gene="pgi"
                     /locus_tag="AC16CX_07700"
     CDS             complement(4703..6355)
                     /gene="pgi"
                     /locus_tag="AC16CX_07700"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3012430.1"
                     /translation="MPSSRTRLPSWQKLTEHQRAVSALHLRELFARDRARFDKFSLRH
                     GGILLDYSKQLVTDETLRLLVALARDCGLESWMKRMFAGERINSTENRAALHVALRAG
                     QPVPLDGADVTRDVKSVLAALGRFARDVQAGRARGATGRAFTDVVNLGIGGSDLGPRL
                     ACEALKPYLRARPRAHFVSNVDGAAVSSVLAALDPRATLIVIASKTFTTQETLANART
                     ATAWLAKKLGGRAAAAKHLCAVTAEPGCAVAFGVPEERVFRIWDWVGGRYSLWSAVGL
                     PIALATGMKCFDELRAGARILDEHFATAPLERNLPVVLGLLEAWNASFRGAASRAVLP
                     YDERLRLLPAYLQQLEMESCGKRVTREGEAVDYATAPVVWGSAGTDGQHAYFQWLHQG
                     TQVAPADFIACCRPHHRLRGNHDLLLANFFAQTEALAFGMGEEEAAAAMRKQGLAETA
                     AQKLLPHRVFEGNRPTTSILLDELTPRTLGTLIALYEHKVFVQSVIWDINAFDQWGVE
                     LGKRLADRILPELSGDAPVSTHDSSTNGLISHFKARRQRGKA"
     gene            6446..7174
                     /locus_tag="AC16CX_07705"
     CDS             6446..7174
                     /locus_tag="AC16CX_07705"
                     /inference="COORDINATES: protein motif:HMM:NF018917.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HIR3012431.1"
                     /translation="MGEVLKAMGGALRDLREPRVLALGLVPPLAAILVWVAAAWAFAD
                     DWARLVADWIAATPWLTWVRDWGLSSVFIWASGIAAVALLLPVMLITAVLVTDIVAMP
                     VIVPFVGERHYPRLERLQGGTVAGSVWNAAAAITVFAALWLATLPLWLTGIGALALPP
                     LISAHFNQRMFRYDALAEHASAAEHEAIMRGAGGRLYLLGLALALLYTIPVFNLVVPV
                     LSGLAFTHLCLGELARHRQEGATR"
     gene            complement(7325..>7650)
                     /locus_tag="AC16CX_07710"
     CDS             complement(7325..>7650)
                     /locus_tag="AC16CX_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3012432.1"
                     /translation="ERYVAAHVKGKGVVVFTACSHAGVINVLKDARNVFGDVPLHAVM
                     GGFHLSGAEVEAIIPDTIRDFGEFALKRIVPCHCTGWRAVRALAQVYNEDILVPAAVG
                     RQFVF"
BASE COUNT         1317 a         2428 c         2571 g         1334 t
ORIGIN      
        1 ctggtgctgg agcaaatgtg gccgttgcgc gagagcaacc gtgtccacta cgggttcaac
       61 cgctacgcgg atttcctgga gatccagttc aacgggggag agcaccggca cggcgtcatc
      121 gcctggatgc tggcggtgct gccggccatc gcgctgacgg cggcggtcta ttacgtgctc
      181 cacggcctga gcccgctcgc cggcttgctg tggagcgtcg tggtgctcta tctcaccatg
      241 ggtttccgcc agttcagtca ttacttcacc gagatccagc aggcgctccg cgcgggcgat
      301 ctcgcgccgg cgcgcgagcg gctcggcaag tggcgcggtg agccggcgtc cgaattcaac
      361 gccagcgaga tcgcgcgcgt ggcgatcgag caagggctgc tcgcttctca ccggcacgtg
      421 ttcggcacga tcgcctggtt cgtggggctg gggccggcgg gcgccgtgtt ctaccgtgcg
      481 tccgccgtgc tcgcggacaa atgggcgacg cgcgctgacc cggaatccgg cgaattcggc
      541 cgcttcgccg ggcgcgcctt tttctggctc gattgggtgc cggcccggct caccgcggcg
      601 agctttgcca tcgtcggcga cttcgaggac tcggtctatt gctggcgcac ccaggccgcc
      661 gcctgggcga cgcaagcgca cggcatcatt ctcgcggcag gcggcggggc gctgggagtg
      721 cgcctcggcg acgcgctgca ccagtacggc agcctgcaat tccggcccga gctgggcgtc
      781 ggcgatgaag cggaggcgga ccacatgcag ggcgcggcgg ggctcatctg gcgcgcgctg
      841 gtgctgtgga tgttcctgat cttcctggtc agcctcgcgc acgcgcttgg ctgaccgcgc
      901 gccgcggtcg ctgagaaact cccctcctcg gacaggaggg gagtcgatcc acgacccacg
      961 attcacggct ttcgttcacg gacaagtccg cgataggatt ccagacgcct ctcgttgatt
     1021 tccccccgct cgcaggccgc cgcgatcgcg caaccgggtt ccgtcacgtg ccggcagtcg
     1081 cgaaatcggc actgccccag gaacggccgg aactcgacga aggcatgcgc aagctcgtcc
     1141 gggtccaggt gatgcagccc gaattcctgc atgcccggcg agtcgatgag ctggctcgcg
     1201 gcatcgagat ggtagaggcg ggcatgcgtg gtggtgtggc gccccgaatc gagggctgcg
     1261 gaaacctccg cgacgcgcac ggatgccccc ggaaccaggg cgttgatgat ggtggacttg
     1321 cccatgcccg actggcccac cagcacgctg gtctggccct tcaggtgcgg cgccagaggc
     1381 ttgagatcct gcctggctga cagcgccagc acgcgatagc cgagccgccg gtagggatcg
     1441 agcgcgccga gcgctgccgg cgcctgcggc aggtcggact tgttgagcac gatcagcgcg
     1501 gccatgcagc cgtgctcggc cgcggccagg cagcggttca acaggtcttc gtaaaacgtg
     1561 ggcaccggcg ccaccacgac gacgatctgc gtcacgttgg cggcgaccag cttctggcgc
     1621 gcgcggtcgg agcggtagag cagcgtcgag cgcggcgcca gcacctcgat cacgccctgg
     1681 ccgcggccgc tgcgggcaac gcttacccga tcgccgcacg cgagggcact gcgcctgccg
     1741 cgcgtgacgc aatcgaggac ggaggcatcg gcaagctcga ccaggtagcg ccgcccgaaa
     1801 ctggccacga tccggccgtt cagcagatgc aatgaagcgt gatgagtgat gggtgatacg
     1861 acttcctgtc aggcgcgcgc cgtgtcacgg atcacttaca tgtcgaaaag cttgtcgagc
     1921 tttgccgcgc agatgaaatc attctcagaa agtccgccga ttgcgtgggt cacgtactcg
     1981 acgcggcact ggttgtagcc gaccgtgatg tcggggtgat ggtcttcgcg gtgggagatc
     2041 cacgcggtgg cattcacgaa cgccatcgtc tggtagtaat tcttgaactg ataggtcttg
     2101 gcaatccgcc cgttcgaata ttcccagccg gcgagctgct tgagcaggtt cccgatctcc
     2161 tgctccttga gcggcgcgac gccgccctcg cagggtttgc acttcttgct ggcgaggtcg
     2221 gagatgatct gggtcatggc atcctctcgg cgagtgattg agtgactaag tgattaggtg
     2281 actgagaaag caattgcatt ccccggggtt gcctcaatca cttaatcacc tagtcactta
     2341 gtcacacctt ttgcaatcgc gcgattcgtg cgattgcggg cggatgcgaa tcgtagaacg
     2401 ccgaatgcag cgggtcgggg gtgagcgtgg aggcgttgtc gcggtagagc ttgatcaacg
     2461 ccgagacgag atcgcgcgcg gaagcgttct gggcggcata gcggtcggcc tcgtactcgt
     2521 gcttacgcga gtacatggcg ccgagcggct gcagcagcac ggtgaactgg ggcaccacga
     2581 tcgagaacag gacgagcgcc atcgccgcgg aaggcgtctg cacgttgagt cccgcgtaga
     2641 accagccctg ctgcatgatc catcccagca gccacaggaa agcgaggctc gccaggccga
     2701 gccatgccag gcgcttcgcg acgtggcgca gcctgaagtg gcccagctcg tgcgccagca
     2761 ccgcctcgac ttctccggga tcgagccgcg cgagcagcgt gtcgaagaag acgatccgct
     2821 tcgacgcgcc gaagccggtg aaataggcgt tgccgtgcgt gctgcggcgc gagccgtcca
     2881 tcaccatcag ccccttcacc ttgaagccgc aacgggcgag caggcgctcg atgcgttcct
     2941 tcagcgccgc atcctgcatc ggcgaaaact tgttgaagag cggcgcgatc cacgtcggat
     3001 agattgcgac gacgaacaga ttgaacgcca cccaggcgag ccaggcgtag atccaccagt
     3061 actcgcccgc ggcggccatc agccacagca cgatcgcgcc gagggggagg ccgaagagca
     3121 gggcgagggc cacgctcttg gcgaggtcga ggaaatagag gccgggcgtc agcttgttga
     3181 acccgaagcg gctttcgatg acgaaggcgc ggtaaagccc gaacggcacg tcgacgagcg
     3241 tcatcagggc cccgaccagc acgagcaaca gcagcccgcg cgcgattcca tcgggcagcc
     3301 acgccgcgga gaccccgtgc agccactgca gcacgccgcc gaaggtgagc gcgagcggga
     3361 cggccacgtc ggctgcgacg ccgaccatcc cgaaacgcgt cttggcgcag gtgtagtccg
     3421 ccgccttctg gtgggcggcg aggctgatct cggcggtgaa ctcgtctggc acgcgcgcgc
     3481 gatgtgcctg gacgtggctg aggtgccgcc gcgcgagcca cagccgcgtc gcggtggcga
     3541 tcgcaagcgc ggcaagaaag acgatcccga aagtgttcat tgcgaagacg gctctgacag
     3601 ggcggtgaaa tctgtgacac aattatcgcc gaaaagcccc cgcctcccaa caaccagtgc
     3661 cgttccaact tgtttcgcca agtggggcga gtatggggca cggggaatct tcaccaattg
     3721 aggcgatccg gatagctgca gatagttgga acggcacaag catggcacag gatcccaaca
     3781 acctcatctg gatcgacatg gagatgagcg ggctcgatcc cgacaacgac aggatcctgg
     3841 aagtggccat ggtcgtgacc gattcgaggc tcgacacggt ggcggaggcg ccggtgctgg
     3901 tggtgcacca gccggacgcg gttctcgacc ggatggacga ctggaacaaa tccacgcacg
     3961 cgaaatccgg gttgatcgaa cgcgtgaagg cttccacgct cggcgtagcg gacgtcgaag
     4021 cgctcatgat cacgttcctg gccgggcacg tgccgccgaa gatctcgccg atgtgcggca
     4081 actccgtgca ccaggaccgg cgctttctcg cgcgccacat gcccgggctg gaggcgtatt
     4141 ttctctatcg caacctcgac gtgagcacac tcaaggagct ggcgaagcgc tggaagccgg
     4201 agatcatggc ggggctcgtg aagcagggca agcacgaggc gctcgccgac atctacgagt
     4261 cgatcgagga gctcaagtac taccgcgaac attttctcaa gctttgacca agcttgagaa
     4321 cgtgagggcg gcagagcgcg agagcgggag ggcgaagcga gactccatct cccgatttcc
     4381 cgatctcacg atttcacggg cgtcgcgtca ctcgaggcgg atttcgccgt cgaacttgac
     4441 gtcaaggccc agtttcccga tcgagagcgt catcgcggcg gggaaagcct cgtttccctt
     4501 gagcttgccg tcggcgatcg ccttgtggat cgccttctcg atcgcgagct gcgagttgac
     4561 gccgacggtc ttgaggaact tgcggatgct catgttcagc gcttcgtcgt tcatggtttc
     4621 ctccataaaa tcggtgaata gtgaatggtg aatagtgaaa ggacaagacc ctttcttcag
     4681 ttcaccgttc actgttcacg gttcacgctt ttcctcgctg acggcgcgcc ttgaagtgac
     4741 tgatcagccc gttggtcgag ctgtcgtgtg ttgacaccgg ggcatcaccg ctgagttcgg
     4801 gcagtatgcg gtcggcgagg cgcttgccca gctccacgcc ccactggtcg aaggcgttga
     4861 tatcccagat caccgattgt acaaacacct tgtgctcgta gagcgcgatc agcgtgccga
     4921 gcgtccgcgg ggtgagctcg tcgagcagga tcgaggtggt cggccggttg ccttcgaaca
     4981 cgcggtgcgg caggagcttt tgagccgccg tctccgccaa tccctgtttc cgcatcgccg
     5041 ccgccgcttc ctcctcgccc atgccgaaag ccagcgcttc ggtctgggcg aagaagttgg
     5101 cgagcagcaa atcgtggttg ccccgcagcc ggtgatgggg ccggcagcag gcgatgaaat
     5161 ccgctggcgc cacctgcgtg ccctggtgga gccactggaa ataggcgtgc tggccgtcgg
     5221 tgccggcgct gccccatacc acgggcgcgg tggcgtaatc gaccgcctcg ccctcgcgcg
     5281 tgacgcgctt gccgcagctc tccatttcga gctgctggag gtacgccggc agcagcctca
     5341 gccgctcgtc gtagggcagc accgcgcggc tcgctgcgcc gcgaaagctc gcgttccacg
     5401 cttccagcag gcccagcacc accggcaggt tgcgctcgag cggcgccgtt gcgaagtgct
     5461 cgtccagaat gcgcgcgccg gcgcgcaatt cgtcgaagca tttcatgccc gttgcaagcg
     5521 cgatgggcag ccccacagcc gaccacaacg aatagcgccc gccaacccag tcccagatcc
     5581 ggaacacgcg ctcctcgggt acgccgaatg cgacagcgca gccgggctcg gccgtgacgg
     5641 cgcacaggtg cttggccgcg gccgcgcgtc cgcccagctt tttcgcgagc cacgctgtcg
     5701 cggtgcgggc attggcgaga gtctcctgcg tggtgaaggt cttggacgcg atcacgatga
     5761 gcgttgcgcg cgggtcgagc gctgcaagga cggacgagac ggccgcgcca tccacgttgg
     5821 aaacgaaatg cgcccgcggc cgcgctctca agtagggctt gagcgcctcg caggcgagcc
     5881 gcgggccaag atcggagccg ccgatgccga ggttgaccac atcggtgaac gcccgtcccg
     5941 tcgcgcccct ggctcgtccc gcttgcacgt cccgcgcgaa acggccgagg gcggcgagca
     6001 cgcttttcac gtcccgggtc acgtcggctc catcgagcgg gacgggctgg ccggcgcgca
     6061 gcgcaacgtg cagcgcggcg cggttctccg tggagttgat gcgctcgccg gcgaacatgc
     6121 gcttcatcca cgactcgagc ccgcagtcgc gggcgagcgc gacgagcaga cggagcgtct
     6181 cgtcggtgac gagctgcttg gaatagtcga gcaggatgcc gccgtgccgc agcgagaatt
     6241 tgtcgaatcg ggcacggtcc cgcgcgaaca gctcccgcag atgcagggcg gagacagcgc
     6301 gctggtgctc ggtgagtttt tgccaggagg gaagccgtgt acggctcgag ggcatcgttg
     6361 cgccagacca gttttgctct cattctaccc gttttcagcg cgccgcctgc ggtcgttgga
     6421 tagaatgtgt ccacagggga tttagatggg tgaagtatta aaagcgatgg gcggcgcgct
     6481 gcgcgacttg cgcgaaccgc gcgtgctcgc gctcggcctg gtgccgccac ttgcggccat
     6541 cctggtgtgg gtcgcggctg cttgggcgtt tgccgacgac tgggcgcggc tcgtcgcgga
     6601 ctggatcgca gccaccccct ggctcacatg ggtacgagat tgggggcttt cctcggtctt
     6661 catctgggca agcggaatcg ccgcggtcgc gctgctcctg ccggtgatgc tgatcacggc
     6721 ggtgctcgtg accgacatcg tcgccatgcc ggtgatcgtg ccgttcgttg gggaacgcca
     6781 ttacccgcgc ctggaacggc tccagggcgg caccgtggcg ggcagcgtat ggaacgccgc
     6841 cgcggcgatc accgtgttcg ccgcgctgtg gctcgccacg ctgccgctgt ggctcaccgg
     6901 catcggcgcg ctggcactgc cgccgctgat ctccgctcat ttcaaccagc gcatgttccg
     6961 ctacgacgcg ctcgccgagc acgcgagcgc cgccgagcac gaggcgatca tgcgcggagc
     7021 cggcggcagg ctttacctgc tggggctggc tctcgccctg ctgtacacca tccccgtctt
     7081 caacctagtc gtgccggtat tgagcgggct cgcgtttacg catctctgcc tcggcgagct
     7141 ggctcgccac aggcaggagg gcgcgacacg atgatgcgtg acgtgatcat gcgtgacatg
     7201 atacggcgtg acatggtgaa gcgtcacgtg atgaggcatt acccgcgagg agtgtgacgc
     7261 ggtgacgatc gatgactcac gacccattgc ttccgtccag agcagagtcg gtgcttgaga
     7321 cggttcaaaa aacgaattgc cttccgacgg ctgccggcac cagaatgtcc tcattgtaga
     7381 cttgcgccag cgcgcgcact gcgcgccagc cggtgcagtg gcaggggaca atccgcttta
     7441 atgcgaattc gccgaagtct cgaatggtat cgggaatgat cgcctcgact tcggcccccg
     7501 agagatggaa gccgcccatc accgcgtgga gcggcacgtc tccgaagacg ttgcgcgcgt
     7561 ccttgagtac gttgatcacg cccgcgtgcg agcatgcggt gaaaacgacg acgcccttgc
     7621 ctttgacgtg cgccgcgaca tagcgctcat
//
LOCUS       DBNGWZ010000259         4189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1790240, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000259 DBNGWZ010000000
VERSION     DBNGWZ010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1790240"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /gene="soxC"
                     /locus_tag="AC16CX_07715"
     CDS             <1..1215
                     /gene="soxC"
                     /locus_tag="AC16CX_07715"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR3012433.1"
                     /translation="AAAMTGYAVTRSAVAEPLSDEPWSKEAGNLVSTYGAPSRFESKV
                     ARTLSNPKGEPRTQHARTPHHLLNGTFTPNGLHFVISHSGDPDIDPDKHRLVIHGLVR
                     RPLVYTLDALHRYPMVSRMTFVECGGNSSPLFSKEPIQATVQALHGLASCAEWTGVML
                     STLLEETGIDPKAKWLIAEGADSLALSRSVPLSKALDDAMIALYQNGERLMPGNGYPM
                     RLLLPGWEGNMNVKWLRRVKLVEQPAMSYYEARTYAPILPDGKAYQFYFLQEVKSFIT
                     HPSFGLRMNGPGFYEISGIAYSGTGRIAKVMVSADGGRTWAKAALQEPVHAKAFTRFR
                     VPWRWDGGPAVLQSRAWDEAGNVQPTRAQIVAHRGQSARTPPVTAFISQHYNSITSWA
                     IDGNGEVKHVYS"
     gene            1202..1732
                     /locus_tag="AC16CX_07720"
     CDS             1202..1732
                     /locus_tag="AC16CX_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3012434.1"
                     /translation="MSTREPLIFAAMLALGSGAVCAQGPNLGKPVSPAEIAAWDISIL
                     PDGSGLPPGSGTPAQGAKIFAEKCVACHSEGGKGGRNVPLVGGAPIRNMDSARTIANF
                     WPYATTLFDFTRRAMPWQQPRTLTNDEVYALTAYILALNKLIGENDVMNAETLPKVRM
                     PNRDGFMPRFPDRMPQ"
     gene            1910..2746
                     /locus_tag="AC16CX_07725"
     CDS             1910..2746
                     /locus_tag="AC16CX_07725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3012435.1"
                     /translation="MATSSIPKLSAHGAEIPVIGFGTSQLGNCGEIVSIALKLGYRHV
                     DTAWKYGTEKGVGEGMRLSGVPRKDIFLTTKVSHEYLRAADFARSVDESLERLQVDYV
                     DLLLVHWPAIDGTPLAETMGALARAKREGKALHIGVANFNIAMTEEAVRLCPEPLCVL
                     QAEYHPYLDQSKVLAFCRKAGLIFMAYCPLARGRLFNDPVLAGIARARGRTLAQVALR
                     WLVQQGNIAPIPRSANPQHMAESLDVFNFSLTDDEMNKIFSLKRPDGRIANPKGRAPK
                     WD"
     gene            2770..3792
                     /locus_tag="AC16CX_07730"
     CDS             2770..3792
                     /locus_tag="AC16CX_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012436.1"
                     /translation="MTIKAKGESRKAEVKQEGSGAWLSFLLSPFAFLLGCALAPATSA
                     QQYPTKPIRLIVPQAPGGSNDIMARSVGQYLAGRLGRQVVVDNRPGAEALIGTDLAAR
                     SAPDGYTLLLASAAYPINAAVRKLPYDPLRAFDWVAMLGSGPTALTVGPLLPVGSVKE
                     LLAAAKAKPGQITMASAGGFQHFAHALFSSLSGQQFTIVLYKGGGPAMIDVMGGQAHL
                     TIGSIVQSLPHIRSGRLKALATGAAKRTTTLPDLPTIAEAGVPGYDASNWWSIAAPAG
                     TPPAIIARLNAEIAAYLNLPETRKRFTDEGAEVDIKTPAELRKMIPAEIAKWTKVARA
                     AGIRVE"
     gene            3884..>4189
                     /locus_tag="AC16CX_07735"
     CDS             3884..>4189
                     /locus_tag="AC16CX_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012437.1"
                     /translation="MNTVPCRFNRIVPAVAVPFVTASLRHSVTMLCLALLAAQQAHAQ
                     AAFPSKNIQMIVPYTAGGSIDLFSRALAQRLTAAWGRNVVVDNRPGASGMIGTEVFTK
                     "
BASE COUNT          758 a         1426 c         1364 g          641 t
ORIGIN      
        1 gccgcggcca tgaccggcta cgcggtgacc cgatccgccg tcgccgagcc gctctccgac
       61 gagccgtgga gcaaggaggc cggaaatctt gtttcgacgt acggcgcgcc gtcgcgattc
      121 gagagcaaag tcgcgcgtac gctcagtaac cccaagggcg agccgcgcac gcagcacgcg
      181 cgcacgccgc accatctgct caacggcacg ttcacgccga acgggctgca cttcgtgatc
      241 tcgcactcgg gcgatccgga tatcgacccg gacaagcacc gcctggtgat tcacggcctg
      301 gtgagacggc ccctggtcta cacgctcgat gcgctgcacc gctatccgat ggtgtcgcgc
      361 atgacgttcg tcgaatgcgg cggcaacagc tcgccgctgt tttccaagga gccgatacag
      421 gccaccgtgc aggcgctgca cggactcgcc tcgtgcgccg aatggaccgg cgtcatgctc
      481 tccaccctgc tcgaggagac cggcatcgac ccgaaggcga agtggctgat cgccgaaggc
      541 gcggactcgc tggcgctgag ccgcagcgtg ccgctgtcca aggcgctgga cgacgccatg
      601 atcgcgctct accagaacgg cgagcggctc atgcccggca acggctaccc gatgcggctg
      661 ctgctgcccg gctgggaagg caacatgaac gtcaaatggc tgcgccgcgt caagctcgtc
      721 gagcagccgg caatgagcta ttacgaggcg cggacctacg cgccgatcct gccggatgga
      781 aaggcctacc agttctattt cctgcaggag gtcaaatcgt tcatcaccca cccgtcgttc
      841 ggcctcagga tgaacgggcc gggtttctac gagatttccg gcatcgctta ttccggcacc
      901 ggccggatcg ccaaggtcat ggtgtcagcc gacggcgggc ggacctgggc gaaggcggcg
      961 ctgcaggaac ccgtgcacgc caaggccttt acccgcttcc gcgtgccgtg gcgctgggac
     1021 ggcggccccg cggtgctgca aagccgcgcg tgggacgaag ccggcaacgt gcaaccgacg
     1081 cgggcgcaaa tcgtggcgca ccgcggacag agcgcgagaa ccccgcccgt cacggctttc
     1141 atcagccagc actacaactc catcacgagc tgggcgatcg acggcaatgg ggaggtcaaa
     1201 catgtctact cgtgaacctc tgatcttcgc cgcgatgctc gcgctcggct cgggcgctgt
     1261 ctgcgcccag gggccgaatc tcggcaaacc ggtcagcccg gccgagatcg cggcctggga
     1321 catcagcatc ctgccggacg gtagcggcct cccgcccggt agcggcacac ccgcgcaggg
     1381 cgcaaagatc ttcgcggaaa agtgcgtcgc gtgccacagc gagggcggca agggcgggcg
     1441 caacgtcccg ctggtcgggg gcgccccgat caggaacatg gactcggcga ggaccatcgc
     1501 caacttctgg ccctacgcca ccacgctgtt cgacttcacg cggcgggcga tgccgtggca
     1561 gcagccgcgg accctcacca acgacgaagt ctacgcgctg accgcctaca tcctcgcgct
     1621 caacaagctg atcggcgaga acgacgtgat gaacgccgag acgctgccga aggtcaggat
     1681 gcccaatcgg gacggattca tgccccgctt tccggacagg atgcctcagt gacgaggtga
     1741 cgggtccgcg ccggctcgag tcacgggtca cgagtcactc gcgacggtgt aatcttcgca
     1801 acgggaatga ccggcgaggc ggtcgcgccg ggcatgcgct gaaggcggaa ggatgaagga
     1861 aaatgtcgtc caccaccgct tcatccgcct tccgccttag aattgcgcca tggcaacgtc
     1921 ttcgattccc aagctgtcgg cgcatggcgc cgaaattccg gtgatcggct ttggcacgtc
     1981 gcaactgggg aactgcggtg agatcgtctc gatcgcgttg aagcttggct accgccacgt
     2041 cgacaccgcg tggaaatacg gcaccgagaa gggggtcggc gaaggcatgc gcctgtccgg
     2101 cgtgccgcgg aaggacattt tcctcaccac caaagtctcg cacgagtatc tgcgcgccgc
     2161 ggatttcgcg cgctcggtgg acgagagcct ggagcgcctc caggtggatt acgtggacct
     2221 gctgctcgtg cactggcccg ccatcgacgg cacgccgctc gcggaaacca tgggtgcgct
     2281 ggcgagagcg aaacgtgagg gcaaggcgct tcacatcggc gtcgcgaatt tcaacatcgc
     2341 gatgaccgag gaggcggtgc ggctctgccc cgagccgctg tgcgtgctgc aggccgagta
     2401 ccacccctac ctcgaccagt ccaaagtgct tgcgttctgc cgcaaggccg ggctcatctt
     2461 catggcctac tgcccgctcg cccgcgggcg cttgttcaac gacccggtgc ttgccgggat
     2521 cgcgcgcgcc aggggcagga cgctcgcgca ggtcgcgctg cgctggctgg ttcagcaggg
     2581 caacatcgcc cccatccccc gctccgccaa tccccagcac atggccgaga gcctcgacgt
     2641 cttcaacttc tcgctgaccg acgacgagat gaacaagatc ttctcgctca agcggcccga
     2701 cggccggatc gccaacccga aggggcgggc gccgaagtgg gactgagtcc gaaggatgaa
     2761 ggaggaagga tgacgatcaa ggcgaaaggc gaaagtcgga aggcggaagt gaagcaagaa
     2821 ggtagcgggg cttggctttc ctttctcctt tcgcctttcg ccttcctcct tggctgcgcg
     2881 ctcgcgccag cgacaagcgc gcagcagtac ccgacgaagc cgatccggct gatcgtgccg
     2941 caggcgcccg gcggcagcaa cgacatcatg gcgcgctccg tcggccagta cctcgccggc
     3001 cgtctcggcc ggcaagtggt ggtggacaac cgtccgggcg cggaagcgct tatcggcacc
     3061 gacctcgccg cgcgctcggc gccggacggc tacacgctgc tgctcgcatc cgccgcctat
     3121 ccgatcaacg cagcggtgcg gaaactgccc tacgatccgc tccgggcctt cgactgggtc
     3181 gcgatgctcg gctccggccc caccgcgctc accgtcggcc ccctgctgcc ggtgggctcg
     3241 gtcaaggaac tgctcgcggc ggccaaggcc aagccgggtc agatcacgat ggcgagcgcg
     3301 gggggcttcc agcacttcgc gcacgcgctg ttcagcagcc tttcaggcca gcagttcacc
     3361 atcgtgctgt acaagggggg tggaccggcg atgatcgacg tcatgggcgg ccaggcgcac
     3421 ctgacaatcg ggtccatcgt acaatcgctg ccccacatcc ggtcgggccg gctgaaggcg
     3481 cttgccaccg gcgccgcgaa gcgcaccacg acgctgccgg atctgccgac cattgccgag
     3541 gccggcgtgc cgggctacga cgcgagcaac tggtggtcca tcgccgcgcc ggcgggcacc
     3601 ccgcccgcca tcatcgccag gctcaacgcc gagatcgcgg cctatctcaa tcttcccgaa
     3661 acccggaagc gattcaccga cgagggcgcc gaagttgaca tcaagacgcc cgcggagctg
     3721 cgcaagatga tccccgccga aatcgccaag tggaccaagg tggcgagggc ggcgggcata
     3781 cgcgtcgagt agatcaggag ccggtgacga ggtgacgcag tgatatgaag tgacggggcc
     3841 agcgccgaac tgcaatttcc atacagaacg aggagagaag accgtgaaca cagttccatg
     3901 ccgattcaac cgcatcgtgc cggcggtggc cgttccgttc gtcaccgcgt cactgcgtca
     3961 ctccgtcacc atgctctgcc tggcgctgct tgccgcgcag caagcccacg cgcaggcggc
     4021 gttcccgtcg aagaacatcc agatgatcgt tccgtacacc gccggcggct cgatcgatct
     4081 cttcagccgc gcgctcgccc agcggctcac ggcggcctgg ggccggaacg tcgtcgtgga
     4141 caaccgcccg ggcgcgagcg gcatgatcgg caccgaggtc ttcaccaag
//
LOCUS       DBNGWZ010000260        10756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_909415, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000260 DBNGWZ010000000
VERSION     DBNGWZ010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_909415"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC16CX_07740"
     CDS             complement(<1..373)
                     /locus_tag="AC16CX_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012438.1"
                     /translation="MRSHTVAILLASALLCPLAMAQDYPTRPIRLVVPFAPGGGSDIA
                     GRILAEGLTQALGQTVVVDNRAGAGSIVGCEIVAKSAPDGYTLLLGNISMAFNAALYR
                     KLPYDTLRDFLPISLVTDQPNI"
     gene            complement(481..1311)
                     /locus_tag="AC16CX_07745"
     CDS             complement(481..1311)
                     /locus_tag="AC16CX_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3012439.1"
                     /translation="MNLRDRIGIDLGRKIRLEEGIEWAAQNGVRYIDIQLDTAENALT
                     TFDAKRCAGVRKRLDQHGVRLGLHTLSAVNVAEYSPYVADAVEQYLKSYVDIYPRLGA
                     SWMVVHAGYHFSKDKEMRMRAGKERLKRIIARAEKKKTLVLLENLNKEPEDAEVHYLA
                     HTVEEWQYYWDIQSPAFKLSFTANHAHLVPEGVAGFVRALDFKRVGEVRLADCYRNGH
                     EIHLAPGKGNLDFGELFRLVEGRGYKGHYTNAFGSLGDMLSARDYLVDKAREAGVNVG
                     "
     gene            complement(1415..2626)
                     /locus_tag="AC16CX_07750"
     CDS             complement(1415..2626)
                     /locus_tag="AC16CX_07750"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3012440.1"
                     /translation="MKHQPGRHFLQIPGPTNVPDRVLRAIDNATVDHRGPEFQETSQE
                     VLAGLKHVFKTKSPVVIFPASGTGAWEAALVNSLSPGDKVLMVETGHFATLWHNMAKK
                     LGLMPEFLPGDWRHGADPAAVEAKLAEDRGHEIKAVCVVHNETSTGVASRIGEVRKAI
                     DRAKHPALYLVDTISSLASIDYRHDEWGVDVTVAGSQKGLMLPPGLSFNAVSEKALAA
                     SKSARLPRSYWNWNEMIAANQDGWFPYTPATNLLYGLRVALRMLLEEEGLENVFKRHD
                     RHAEATRRAVRAWGLEILCLNPAEYSSSLTAVLMPEGHSEIAFRGLVLERFNLSLGSG
                     LTKLRDKVFRIGHLGDFNDLMLLGTLAGIEMGLELARVPHKKGGVDAAMEYLVRGEQA
                     QAKGGAKARVA"
     gene            2829..5747
                     /locus_tag="AC16CX_07755"
     CDS             2829..5747
                     /locus_tag="AC16CX_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR3012441.1"
                     /translation="MRKIDPGDSSLAARLKREIHGDVLFDAASRGRYSTDASIYQIEP
                     VGVVVPATEEDALTALRIAIEEGVPVLARGGGTSQCGQTVGAALIIDHSKHLNRIVAF
                     DPEARAVTVQPGVVLDQLNAYLRPHGLWYPVDVSTSAQATIGGMAGNNSCGSRSIHYG
                     NMVHNVRAIDAVLADGSEYAFGEVKGEVDRLAGPAGYRELVKKVRAIAAREAEEIEHR
                     VPKLLRRVGGYNLDLVSGIPFNMAHLLVGSEGTLAYSHRIHLNLSPLPKHKTLGVAHF
                     PAFYQAMETAQHIVKLGPVAVELVDRTMIGLARGNAAFRSTVEQAIKGDPDAILLVEF
                     AGEERDGPLRKLKQLTGLMGDLGFPGSVVEITDGALQRDVWEVRKAGLNIMMSMKGDG
                     KPVSFIEDCAVPLEHLAEYTERLTRVFEKHGTKGTWYAHASVGCLHVRPVLDMRKEGA
                     TQMRAIAEEAAELVKQYKGAYSGEHGDGLVRSEWIEPFFGPKLTRAFGEIKELFDPKG
                     LMNPGKIVRPSRQDDRSLFRYKPGYRTIPLKTALDWSEYETDGGQRGEGFAKAVEMCN
                     NNGHCRKFDAGTMCPSYRVTREERHLTRGRANTLRLALSGQLGPDALASDELYATLDL
                     CVSCKGCKRECPTGVDMAKMKVEFLYHYRKRHGLRLKDRLIAYLPRYAPLAARLAPLV
                     NAAQGTAKGWLGLAPQRSLPKWRRDAFYENHPHPSLPPEREGDSSREAVLMIDTFNRY
                     FEPENARAALAVLHAAGYAVHLPRGTDGRRPLCCGRTFLAAGLVDEARFEARRVVETL
                     KPYVERGVPVVGLEPSCLLGLRDEFLSLLPGGESAELAMNAFLFEEFLAHEHGAGRLK
                     LDLKPLPQKKALLHGHCHQKAFAVMPDVERVLRLVPGLEVETIESSCCGMAGAFGYEA
                     AHYEVSMKMAETSLLPKVRKAGGDELVVADGVSCRHQIHDGSGRAAMHVARVLERSLA
                     "
     gene            5744..6313
                     /locus_tag="AC16CX_07760"
     CDS             5744..6313
                     /locus_tag="AC16CX_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3012442.1"
                     /translation="MTPVTRHESPVFAGAAGVSLILKALEFAALKHRDQRRKDKDASP
                     YINHPIALANVLWQEGGVTDPLVIAAALLHDTIEDTETTWQELRGGFGDEVAEVVLEV
                     TDVKWLKKNVRKRLQVAKARHASERARLVKLADKICNLRDVAAHPPAGWDLARRRGYF
                     EWAKEVVDRLRGTHRELERRFDDVYALKP"
     gene            complement(6332..7330)
                     /locus_tag="AC16CX_07765"
     CDS             complement(6332..7330)
                     /locus_tag="AC16CX_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012443.1"
                     /translation="MSSLNKAFRTAAALCAGACLAQPLAAAQADAAKDYPNKPIRFIV
                     PFVPGAGTDTTARTIAQKLSEMWGQQVVVDNRTGAAGAIGVELTAKAAPDGYTICLIS
                     ASHSVNAAVNQKLPYDLTKDLAAISQASSLFYAVYHHPSVPVKSIRELIAYAKANPGK
                     LNFGSSGTGSLQHFAGEMFNHMTGAKLVHVPYKGTAAVITAMLAGDVQVGFGTLFGVR
                     PHMASGRLKVLAITAGKRSPAAPELPTVAEAGVPGYVVDQWYGVITSAKVPAPIVRKL
                     SAGIAEALKSPDVAKRFAADGSTPTGSTPEQFSAHIRSEIAKWRKLAKEAKLELQQ"
     gene            7543..8517
                     /locus_tag="AC16CX_07770"
     CDS             7543..8517
                     /locus_tag="AC16CX_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012444.1"
                     /translation="MHSGKIVLGAAGMLLGALIASAAGAQPYPAKAVRILTGQAPGGA
                     TDLFARLFAQKLNEAWKQPVVVEPRPGAAGAIAAELTSKSAPDGHTLLLSTAGQVVIN
                     PHLTRLSYDALKDLAPVVYLTGSSLLLVTHPSVPVRSVKELVALAKARPGRLNHGSGG
                     SGSPAHLAMELFKYMTGTKMTHIPFKGVGPAVTALIAGEIDLSFASVASTQAFVKAGR
                     LRPLAISTPKRSRALPEVPTVAEAGVPGYEVTTWYGFFGPAAMPSEIVNKIHADVTRL
                     LGQPDVRDRLLAEGGEPGNLTVEQFAAFTRAEYAKWGKVIRAAGIKGE"
     gene            8567..9265
                     /locus_tag="AC16CX_07775"
     CDS             8567..9265
                     /locus_tag="AC16CX_07775"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3012445.1"
                     /translation="MNAVKQKLAAGKVVTMVNPDFQHPRLVEYLARLGMDVSFIDAER
                     MSYDFERIEEMTRAAHLAGIASVARAWMNEPGLITRCFDCGLDGMSFPHIEDAATAKR
                     MVEIVRYARPKDHADKIVIIMVETPDAIGRLDEIVAVPGVDVVNIGVNDVAFALGRPG
                     EPEHPEVAKLVDRAIERILRGGKHVALNVLDNWEERMPAFVAKGVRWLNVHASAFIAR
                     GAKQYAGLLSRCAP"
     gene            complement(9426..10055)
                     /locus_tag="AC16CX_07780"
     CDS             complement(9426..10055)
                     /locus_tag="AC16CX_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012446.1"
                     /translation="MKSKRRIAVLIALMLAMPVGIAALEETASSDSAEATETPAVQAE
                     TPAVQAESTAQPAELSAPPMAESVVEAVVASAPVERLVRATPSDTFPVGSLDSNYFEP
                     LPSQIAYFERLEQERRHLVARGDVFPEGTEVEWRMLPTQIAYFDGLERQHLASAEQMT
                     QSDAMSDSAAATGESRNPLRVAWDYVSGLFKRMGAAISGWGSSSESAVQ"
BASE COUNT         1867 a         3435 c         3612 g         1842 t
ORIGIN      
        1 ggatgttcgg ctggtccgtc accagcgaga tcggcaggaa atcgcgcagc gtgtcgtacg
       61 gcagcttcct gtagagcgcg gcgttgaacg ccatgctgat gttgccgagc agcagcgtgt
      121 agccgtcggg cgcagacttg gccacgatct cgcagccgac gatgcttccc gccccggcgc
      181 ggttgtccac cacgaccgtc tggccgagcg cctgcgtcag cccttccgcg agaatgcgtc
      241 ccgcaatgtc gctgccgccg ccgggcgcga acggcaccac gaggcggatc ggccgtgtgg
      301 gatagtcctg cgccattgcc agcgggcata gaagggcgga tgccagcaga atcgcgacgg
      361 tgtgtgatct catacagtat gtgggatgaa ggatgaggaa tgacccgggc gcttggctca
      421 aggatgaaag aaaacccgcg tcttacaggt cgtccttacg ccttcatcct tccgttggcg
      481 ttagccgacg ttaacgccag cctcgcgcgc cttgtcgacc aggtagtcgc gggccgagag
      541 catgtcgccg agcgagccga acgcgttggt gtagtgaccc ttgtagcctc tgccctcgac
      601 cagccggaac agctctccga agtcgagatt gcccttgccg ggggcgagat ggatctcgtg
      661 gccgttacgg tagcaatcgg cgagccgcac ttcccccacc cgcttgaagt cgagcgcccg
      721 cacgaacccc gcgacgccct cgggcaccag gtgcgcgtgg ttggcggtga aggaaagctt
      781 gaaggcgggc gactggatgt cccagtagta ctgccattcc tcgacggtat gcgcgaggta
      841 gtggacttcc gcgtcctccg gctccttgtt gaggttttcg agcagcacca gcgtcttctt
      901 cttctcggcg cgggcgatga tgcgcttcag ccgttccttg ccggcacgca tgcgcatctc
      961 cttgtccttg gagaaatggt agcccgcgtg caccaccatc cagctcgccc cgagcctcgg
     1021 gtaaatgtcc acgtagcttt tcaggtactg ctccactgcg tcggcgacgt aaggcgagta
     1081 ctcggcgacg ttcaccgccg agagcgtgtg cagccccaac cggacgccgt gctggtcgag
     1141 ccgcttgcgc acgccggcgc agcgcttcgc gtcgaaggtg gtgagggcgt tttccgcggt
     1201 atcgagctgg atgtcgatat agcgcacgcc gttctgcgcc gcccactcga tcccttcctc
     1261 gaggcggatc ttgcggccca ggtcgatgcc gatgcggtcg cgcagattca ttgcgggtga
     1321 ttgagtgatt aagtgattag gtgattaagt aaccgggcga ccaagcgtat tggctcaatc
     1381 actcaatcgc taagtcactt aatcactggc ctgctcaggc gacgcgggct ttcgcgccgc
     1441 ctttcgcctg cgcctgctcg ccgcgcacga ggtattccat cgccgcatcg accccgccct
     1501 ttttatgcgg aacccgggca agctccaacc ccatctcgat gccggcgagc gtgccgagca
     1561 gcatgaggtc gttgaagtcg ccgaggtgac cgatgcggaa caccttgtcg cgcagtttgg
     1621 tgaggccgga gccgagcgag aggttgaaac gctccagcac gagcccgcgg aaggcgattt
     1681 cactgtggcc ttccggcatc aacacggcgg tgagcgagct cgagtactcg gccggattca
     1741 ggcacaggat ctcgagaccc cacgcgcgca ccgcgcggcg ggtcgcctcg gcgtggcgat
     1801 cgtggcgctt gaacacgttc tccagccctt cctcttccag cagcatcctg agcgcgacgc
     1861 gcaggccgta gagaagattg gtcgccggcg tgtacgggaa ccagccgtcc tggttggcgg
     1921 cgatcatctc gttccagttc cagtacgagc gcgggagccg cgcggacttc gacgcggcga
     1981 gcgccttttc ggaaaccgcg ttgaacgaaa gcccaggcgg gagcatcagg cctttctggg
     2041 aaccggccac ggtcacgtcc acgccccatt cgtcgtggcg gtaatcgatg gaggcgagcg
     2101 aggagatcgt gtccaccagg tagagcgccg ggtgcttcgc gcggtcgatc gccttccgca
     2161 cttcgccgat gcgcgaagcg acgccggtgg aggtctcgtt gtgcacgacg cacaccgcct
     2221 tgatctcgtg cccgcgatcc tcggcaagct tggcttcgac tgcggccggg tcggctccat
     2281 gtcgccagtc tccgggtaaa aactcgggca tcaacccgag ttttttagcc atgttgtgcc
     2341 acagcgtcgc gaagtggccg gtctcgacca tcagcacctt gtcgccgggc gagagcgagt
     2401 tgaccagcgc ggcttcccac gcgccggtgc cggaggcggg gaagatcacc accggcgact
     2461 tcgtcttgaa cacgtgcttg agtcccgcca gcacctcctg gctcgtctcc tggaactcgg
     2521 ggccgcggtg gtccacggtc gcgttgtcga tcgcgcgcag cacgcggtcc gggacgttgg
     2581 tgggaccggg gatctgcagg aaatggcggc cgggttgatg cttcatatga gtctccaggg
     2641 agggtgcgtt attataggcc caggcattgg cgatgataga aacgcccaaa aggatgaagg
     2701 atgagggatg accccgcggc gcgggcgtcg caacctagcc atccattcgc ctttcgtttc
     2761 ggtttttgat ctctgagcga agcgaaggga tcatccttca tccttccgcc tttatccttc
     2821 gcatgaccat gcgcaagatc gaccccggtg attcgtctct cgccgctcgt ctgaagcgcg
     2881 agattcatgg cgatgtgttg ttcgacgccg cctcgcgcgg gcggtattcc accgacgcct
     2941 ccatctacca gatcgagccc gtcggcgtgg tggtgccggc gacagaagag gacgctctca
     3001 cggcgctgcg catcgcgatc gaggagggcg tgccggtgct cgcgcgcggc ggcggcacct
     3061 cgcagtgcgg ccagaccgtc ggcgccgcgc tcatcatcga tcactcgaag cacctcaacc
     3121 ggatcgtcgc gttcgacccg gaagcgcgcg cggtgaccgt gcagcccggc gttgtgctcg
     3181 accagctgaa cgcgtacttg aggccccacg ggttgtggta cccggtggac gtctcgacca
     3241 gcgcgcaggc gacgatcgga ggcatggcgg gcaacaactc gtgcggctcg cgctcgatcc
     3301 actacggcaa catggtgcac aacgtgcggg cgatcgacgc cgtgctcgcc gacggctccg
     3361 agtacgcgtt cggcgaggtg aagggcgagg tcgatcggct tgccggcccc gccggctacc
     3421 gggagctggt gaagaaggtg agggcaatcg cggcgcgcga ggcggaggaa atcgagcacc
     3481 gcgtgcccaa gctcctgcgc cgcgtcggcg gctacaacct ggacctagta agcggcatcc
     3541 ctttcaatat ggcgcacctg ctggtggggt cggaaggcac gctcgcgtat tcgcaccgta
     3601 tccatctcaa cctctcgccc ctgccgaagc acaagacgct cggcgtcgcc cactttcccg
     3661 ccttctacca ggcgatggag actgcgcagc atatcgtgaa gctcggcccg gtggcggtgg
     3721 agctggtgga ccgcaccatg atcgggctcg cgcgcggcaa tgccgcgttc cgctcgaccg
     3781 tcgagcaggc gatcaaaggc gaccccgacg cgattctgct ggtggagttc gcgggtgagg
     3841 agcgggacgg gccgctgagg aagctcaaac aactgaccgg gctgatgggc gacctcggct
     3901 tccccggcag cgtcgtggag atcaccgatg gggcgcttca gcgcgacgtg tgggaggtga
     3961 ggaaggcggg actcaacatc atgatgtcca tgaagggcga cggcaagccc gtgtccttca
     4021 tcgaggactg cgcggtgccg ctcgagcatc tcgccgaata caccgagcgc ctgacgcgcg
     4081 tgttcgaaaa gcacggcacg aaaggcacgt ggtacgcgca cgcttccgtc gggtgcctgc
     4141 acgtgcggcc ggtgctcgac atgcgcaagg agggcgcgac gcagatgcgc gcgatcgccg
     4201 aggaggcggc ggagctggtg aagcagtaca agggcgccta ttccggcgag catggagacg
     4261 gcctggtacg ctcggagtgg atcgagccct tcttcggtcc caagctcacg cgcgcgttcg
     4321 gcgagatcaa ggagctgttc gacccgaagg ggctcatgaa tcccggcaag atcgtgcggc
     4381 cctccaggca ggacgaccgc tcgctcttcc gctacaagcc gggctaccgg acgatccccc
     4441 tcaagaccgc gctcgactgg tcggaatacg agaccgacgg ggggcagcgg ggagagggct
     4501 tcgccaaggc ggtggagatg tgcaacaaca acggccactg ccgcaaattc gacgccggca
     4561 ccatgtgccc gtcgtatcgc gtgacgcgcg aggagcggca tctcacgcgc gggcgcgcca
     4621 acacgctgcg gctcgcgctc tccggacagc tcgggccgga cgcgctcgcc tcggacgagc
     4681 tctacgcaac gctcgacctc tgcgtctcgt gcaagggctg caagcgcgag tgcccgaccg
     4741 gcgtggacat ggcgaagatg aaggtggagt tcctctacca ctaccgcaag cgccacggtc
     4801 tcaggttgaa ggaccgcctc atcgcatacc taccgcgcta tgctccgctt gccgcccgcc
     4861 tcgcgccgct ggtcaacgcc gcgcagggca ctgcgaaggg ctggctgggt ctcgcgccac
     4921 agcgctcgct gccgaagtgg cgccgcgacg cattttatga gaaccacccc catcccagcc
     4981 ttccccctga gagggaaggg gattcgagcc gcgaggcggt attgatgatc gacaccttca
     5041 accgctattt cgagcctgaa aacgcccgcg ccgcgctcgc ggtgctgcat gcagcgggct
     5101 acgccgtgca cctgccgcgg ggcacggacg gccgccgtcc gctctgttgc gggcgcacct
     5161 ttcttgcggc cggcctggtg gacgaagcga ggttcgaagc gcgccgcgtg gtcgaaacct
     5221 tgaaacccta cgtcgagcgc ggcgtgccgg tggtcgggct ggagccttcc tgcctcctgg
     5281 gcctgcgcga cgaattcctc tccctgctgc cgggtggcga gagcgccgaa ttggcgatga
     5341 acgcattcct gttcgaggag ttcctcgcgc acgaacacgg ggcggggcgc ctgaagctcg
     5401 acctcaagcc gttgccgcag aagaaagcgc tcctgcacgg tcactgccac cagaaggcgt
     5461 tcgctgttat gcccgatgtc gagcgcgtgt tgaggctcgt cccggggctg gaagtggaaa
     5521 ccatcgaatc gagctgctgc ggcatggcgg gcgccttcgg ctatgaggcg gcgcattatg
     5581 aagtctcgat gaagatggcc gaaacctcgt tgctcccgaa ggtgcgaaag gcgggcggtg
     5641 acgagctcgt cgtcgccgac ggcgtgagct gcaggcatca gatccacgac ggcagcggtc
     5701 gcgcggcgat gcatgttgcc cgggtgctcg agcgatcgct ggcatgacgc ccgttacccg
     5761 tcacgagtcg ccggttttcg ccggcgcggc gggggtctcg ttgatcctga aagcgctcga
     5821 gttcgccgcg ctcaagcacc gcgaccagcg gcgcaaggac aaggacgcct cgccctacat
     5881 caaccacccg atcgcgctcg cgaacgtgct atggcaggag ggcggcgtga ccgatcccct
     5941 ggtgattgca gcggcgctgc tgcacgacac catcgaggac accgagacca cctggcagga
     6001 gctgcgcggc gggttcggcg acgaggtcgc cgaggtggtg ctcgaggtca ccgacgtgaa
     6061 atggctcaag aagaacgtgc gcaagcgcct ccaggtcgct aaggcgaggc atgccagcga
     6121 gcgggcgcgc ctcgtcaagc tcgccgacaa gatctgcaac ctgcgcgacg tcgccgccca
     6181 cccgccggcg gggtgggacc tcgcgcgccg gcgcgggtac ttcgagtggg caaaggaggt
     6241 cgtggatcga ctgcgcggca cgcaccggga gcttgagcgc cggttcgacg atgtctacgc
     6301 gctcaagccg tgacgggtga cgcgtgacgg gttattgctg caactccagc ttcgcttcct
     6361 tcgcgagctt gcgccacttg gcgatctcgg agcggatgtg cgcgctgaac tgctcgggcg
     6421 tcgagccggt cggcgtggag ccgtcagcgg cgaagcgttt cgccacgtcc ggggacttca
     6481 gcgcctcggc gatccccgcg gacaatttgc ggacgatcgg cgcgggcact ttggcggacg
     6541 tgatcacgcc gtaccactgg tccaccacgt agccgggcac gccggcttcc gcgacggtcg
     6601 gcagctccgg cgcggcgggc gagcgcttgc ccgctgtgat cgccagcact ttcaggcggc
     6661 cgctcgccat gtggggccgc acgccgaaca gcgtcccgaa gccgacctgg acgtcgccgg
     6721 ccagcatggc ggtgatgacg gccgccgtgc ccttgtacgg cacgtggacg agcttcgcgc
     6781 cggtcatgtg attgaacatc tcgcccgcga agtgctgcag gctcccggtg ccggacgacc
     6841 cgaaattgag cttgccgggg ttcgccttgg cgtacgcgat cagctccctg atcgatttca
     6901 ccgggaccga ggggtggtgg tagaccgcgt agaaaaggga ggacgcctgc gagatcgccg
     6961 cgaggtcctt cgtgaggtcg tagggaagct tctggttgac ggcggcattg acggaatgcg
     7021 acgccgagat caggcagatc gtatagccgt cgggggccgc cttcgcggtg agttccacgc
     7081 cgatcgcgcc cgcggctccc gtccggttgt ccacgacgac ctgctggccc cacatttcgg
     7141 aaagcttctg ggcgatggtg cgcgcggtgg tgtcggtgcc ggctcccgga acgaaaggca
     7201 cgatgaagcg gatcggcttg ttcgggtaat ccttcgcggc gtctgcctgc gcggcggcga
     7261 gcggttgggc gagacaggcg ccggcgcaca acgccgccgc cgtccggaag gctttgttca
     7321 gggaagacat ggggatttcc tccgttatcg tccaggccgt atcttactat cagtgacgag
     7381 ccacgagcct gcaatccggc ggtaggttgc aagtgacagg cgatacgcat acaggccggc
     7441 gagccggacg ttcgtatcac ctgtcacgta ttacgccaaa atgcagtaaa gtgcccgcag
     7501 cgcatccaga aaaatcagaa cgcgaaccct gaggggaagg acatgcactc cggaaaaatc
     7561 gtcctcggcg cggccggtat gctgctcggc gcattgatcg cgagcgcggc cggcgcgcaa
     7621 ccgtacccgg caaaggcggt gcgcatactc accggacagg ctccgggcgg cgccactgac
     7681 ttgttcgcgc ggctgttcgc gcagaagttg aacgaggcgt ggaagcagcc ggtggtagtg
     7741 gagccccgcc ccggcgcggc gggcgcgatc gccgccgagc tgacgtcgaa atccgcgccc
     7801 gacggccaca cgctgctgct ctcgaccgcc gggcaggtcg tgatcaaccc gcatctcacc
     7861 cggctgtcct atgacgcgtt gaaggacctc gcgccggtcg tgtacctcac cggctcatcg
     7921 ctgctgctgg tgacgcaccc ttcagtgccg gtgagatcgg tgaaggagct cgtcgcgctg
     7981 gcgaaggcgc gccccggccg gctcaaccac ggctccggcg gcagcggctc gccggcgcac
     8041 ctcgcgatgg agctcttcaa gtacatgacc ggcacgaaga tgacgcacat cccgttcaag
     8101 ggcgtgggcc cggcggtgac ggcgctgatc gccggcgaga tcgatctctc gtttgcttcc
     8161 gtcgcttcca cgcaggcgtt tgtgaaagca ggccgtctgc gccccctggc gatctcgaca
     8221 cccaagcgct cgcgagcgct gccggaggtg ccgacggttg cggaggccgg cgtgccgggc
     8281 tacgaggtca cgacctggta cggcttcttc gggccggcgg cgatgccctc cgagatcgtc
     8341 aacaagatcc acgccgacgt cacgcgcctc ctcggccagc cggacgtgcg cgaccgcctt
     8401 ctcgccgagg gcggagagcc gggcaatttg acggtcgagc agttcgcggc gttcacccgc
     8461 gccgagtacg cgaagtgggg aaaggtgatc cgcgccgcgg gaattaaagg cgaatgaagc
     8521 gtgacatggt caggcgtgac atgatgctgt gtgacaagga aatgaaatga acgcggtcaa
     8581 acagaagctg gcggctggaa aagtggtgac gatggtgaac cccgatttcc agcacccgag
     8641 actcgtcgag tatctcgcca ggcttggcat ggacgtctcg ttcatcgacg ccgaacgcat
     8701 gagctacgac ttcgagcgca tcgaggagat gacgcgcgcg gcgcacctgg cgggcatcgc
     8761 ctccgtggcg cgcgcatgga tgaacgagcc ggggctcatc acgcgctgct tcgactgcgg
     8821 cctggacggc atgtcttttc cgcacatcga ggacgccgcg accgcaaaaa ggatggtcga
     8881 gatcgtgcgc tacgcccggc ccaaggacca tgccgacaag atcgtcatca tcatggtcga
     8941 gacgccggac gcgatcggac ggctcgacga gatcgtcgcc gtgcccggcg tcgacgtggt
     9001 gaacatcggc gtgaacgacg tggcgttcgc gctgggccgc cccggcgagc cggagcaccc
     9061 cgaggtcgcg aagctggtcg accgggcgat cgagcgcatc ctccggggcg ggaagcacgt
     9121 cgccctcaac gtgctcgaca actgggagga gcgcatgccg gcattcgtcg ccaagggcgt
     9181 acgctggttg aacgtccacg ccagtgcctt catcgcgcgc ggcgcgaagc agtatgccgg
     9241 gctcctcagc cggtgcgcgc cgtgaggagc gagggatgag gaatgaggga tgaagattga
     9301 ggatcgatga aaagcacttg ccatccgcca ctcacgcttc gtgatcaaat aaaaagggcg
     9361 gccgcagccg ccctttgtcg ccggtgacgg gcggtcagtc gctcgtcaca atgcaccctc
     9421 aagcgttact gaacggcgct ctcggaggaa ctgccccagc ccgagatcgc cgcgcccatc
     9481 cgcttgaaca gcccgctgac atagtcccag gccacccgca gcggattgcg tgattcgccg
     9541 gtagcagccg ccgagtccga catcgcgtcg gactgcgtca tctgctcggc gctggcgaga
     9601 tgctgccgct cgagcccgtc gaagtaggcg atctgcgtgg gcagcatccg ccactcgacc
     9661 tcggtacctt ccggaaacac gtcgccccgc gcgaccaggt gacggcgttc ctgttcgagc
     9721 cgctcgaagt aggcaatctg ggacggcagc ggttcgaagt agttgctgtc caaagaaccc
     9781 acgggaaacg tatcgctcgg cgttgcccga acgagccgtt cgaccggcgc gcttgcaaca
     9841 actgcttcga cgacgctctc ggccatgggc ggcgcagaga gctcggccgg ttgtgcagtg
     9901 gactcggcct ggacggctgg cgtctcggcc tggacggctg gcgtttccgt tgcctcagcg
     9961 gaatcgctcg aagcggtctc ttccagggcc gctatgccga ccggcatggc gagcattaaa
    10021 gcgatcagga cggcgatacg acgtttcgac ttcatggcgg ttctcctgat agtggattga
    10081 agaaggttca ccagtacacc tcgcgactga agcgataccc atgccatgcg tgaaaacgcc
    10141 atgaatacat gaagttgcgg gcaaccttgg gagacgaggt gtcgttagag cacgacgagg
    10201 cgggaatcgc tttgcttaat cgcctgattt gtaagtcgtc tcgttgtcgt ttgttcgcga
    10261 caacggtgac gcggggcgga tgataaatga aagcagacga atgatgacag atgcctcacg
    10321 gagcgcgtgg ttaagctacc catcaccgat cactcatcac cccgcggcct ctgattggga
    10381 ggaggcaagt atcgcgcctc atcgtgtcgg ccgcctgctc gcttccgcgg tcgcacccgc
    10441 aagcggggcg gctggcgagg tggagcaggg gccccatcgt taatggggat gcgacccgag
    10501 atcagacgca aagggaccgc cgtcccatga ccgcctccga ggtcagatcc ccataccaaa
    10561 aacgtatggg gcccctcttc cactctccgg cggtccctgc tccgcgctcc agcgcgctac
    10621 tccgccttga ttcccgcctc gcggatcacc ttgccgagcc gggcgttgtt gtccttgatg
    10681 acggcggcga acgcgtccgg cgagccgccg acaacatcga agcccatcgc ggtgagccgc
    10741 tcgcgtatgt cgggca
//
LOCUS       DBNGWZ010000261         7685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2042586, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000261 DBNGWZ010000000
VERSION     DBNGWZ010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2042586"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..2780
                     /locus_tag="AC16CX_07785"
     CDS             12..2780
                     /locus_tag="AC16CX_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3012447.1"
                     /translation="MANLPSGTVTFLFTDIEGSTRLLQQLGEKFATLITEQQQILREA
                     CETHNGRVVSIQGDSFFMAFPSAVDAINAVVQSQRELAAHRWTNGVSVRVRMGLHTGE
                     SQINASDYVGIDVHRAARIAAAAHGGQVLLSQTTLDLIQNDLPDEVTTRDLGNHRLKD
                     LRQPKHLYQLVINDLPSSFPPIKSLNALANNLPIQLTSFIGREKEIRGVKQVILDHRL
                     VTLTGLGGSGKTRLALQAAAEMIEHFHNGVFFVALAPITDPGLVASTIAQAIGITESG
                     GQSIIDGLKDYLQGKSLLLLLDNFEQVISAAALVAELLVACRELKVLVTSREGLRIGG
                     EHEYPVPPLALPILTQLPSLEALTQYAAVKLFIQRAQAVKPDFNITNDTAPTVAEICY
                     RLDGLPLAIELAAARIKLLSPRAMLARLENRLEFLTGGGRDLPARQQTLRNAIAWSYD
                     LLSEEEQKLFRRLSVFVGGSTLEAVEAVEAVAGDQSARVSVLDQLGSLLDKSLLREVE
                     GTDGEPRFVMLELLREFGLEQLEGSGEQEAIRGRHANFCLALAEGAEASLESAEQVQW
                     MDRMEQEHDNLRAALEWSKTADGTGELCLRLAGALGSFWEVRGHFSEGRERLSAILST
                     KSAQGQTAARARLLARAAELAYRQSVYPATTSLAKESLAIYRKIGDEQGIASTLIKLG
                     NAATERGSYATASRFLEEALTIWRERKDKHGTARALISLGWAALRSGDTHLANTRLEE
                     ALALSRELGDARSMGFELSGLGEVALRQGDYARATQLMEESLELRRQLGNKWGVGVSL
                     GMLGWVAMRERDWDRATARLGESLQVRQEIGDKGGSAWCLERLAGIAIVQGQAEKAVR
                     LFSAAAALRASIGSVIDPVDRASYERNRRSLRAKLGQERFKAAWDEGCAMKMEQAIAY
                     ALGTSDAQ"
     gene            2780..2971
                     /locus_tag="AC16CX_07790"
     CDS             2780..2971
                     /locus_tag="AC16CX_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3012448.1"
                     /translation="MFSLASTRSQPAKTSHSVWVDWVDFYGQTRTVDVHIAHLRKILA
                     GGQVSIETVTGVGYKLVVA"
     gene            2972..3646
                     /locus_tag="AC16CX_07795"
     CDS             2972..3646
                     /locus_tag="AC16CX_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR3012449.1"
                     /translation="MSEKPKKPFDIDLAIERIRAAVRPFPKAAMFELAEEGFASPFEL
                     IVACVISIRTYDEVTLPTALKLFAQARTPAAICQLTVAQLDELIRASSFHERKAQQIH
                     QIAQRTVKDYDGQLPCDREVLLSLSGVGPKCANLALGIACNEPFIGVDIHVHRVTNRW
                     GYVAATTPERTLAVLETKLPRAYWVEINRLLVPFGKHICTGTMPRCSTCPLLDMCQQV
                     GVKAQR"
     gene            complement(3703..4329)
                     /locus_tag="AC16CX_07800"
     CDS             complement(3703..4329)
                     /locus_tag="AC16CX_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR3012450.1"
                     /translation="MKRILVAYATMAGSTVDVARVVGEEIAKSGMQVDVLPLGEVKDL
                     EAYDGVVVGGPMIMGWHRAALGFLKKHRGAFQRIPLAVFVMGMSLTQTGETSVGGVPV
                     CVDEKLPKPPEKEGRLTFRERYARLSSYIQPILTATRPVKPASIGFFGGRLEYGRLKW
                     WAVLFVMVIIQAQAGDRRNWTAIRSWAAGLPAALRLETPEADGLAQAA"
     gene            complement(4388..4879)
                     /locus_tag="AC16CX_07805"
     CDS             complement(4388..4879)
                     /locus_tag="AC16CX_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3012451.1"
                     /translation="MSKIDTLVQDFLAQKRIAVVGVSDKRETGCNLGYRKFKGAGYAV
                     SAVNPHLTTFEGDPCYPDLRSIPEKPDAVFILANPKVTEQIVQQCVDLGIRRVWMHCM
                     MGTKPGLAAGMTSVSQGAAQMCRENGITVIPGACPNQFLNPDFGHAMMRVLWRSLGFL
                     SVN"
     gene            5204..5404
                     /locus_tag="AC16CX_07810"
     CDS             5204..5404
                     /locus_tag="AC16CX_07810"
                     /inference="COORDINATES: protein motif:HMM:NF021552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR3012452.1"
                     /translation="MCRSIKQLRRPEQPPTDEELRAAALQFVRKVSGYRAPSKANEEA
                     FNRAVAEIAAATRVLLDNLVVR"
     gene            5463..6947
                     /locus_tag="AC16CX_07815"
     CDS             5463..6947
                     /locus_tag="AC16CX_07815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3012453.1"
                     /translation="MNSLSLRLFAAFLVIILLVTLVTGLALILILRNTPLVQRQALTR
                     LNEAARAALQQGLPPDDPSPEAAAQYVKQLGDTFEVRVLMAGSGGAVIADSASAASPL
                     KLNFRLSTVDPAAPGARSGRARDGNLQRWVYVARSVGSGRVLVFALQEPRFAALAFFA
                     ENLLLPLLEAGAIATLVALALAVLIARSIALPLQKMAAVAQGIARGDHSQAAPVSGPD
                     EVRALGQSLNQMSTQVQVTQQAQRDFLANVSHELKTPLTSIQGFAQAMLDGAADSPQA
                     IQRSANVIYTEADRLRRLVEGLLDLARLDVGLRALSRAPLDLRLVIANVVEKFGLRAK
                     EKGVTLRGELPPALPAMVGDADRLAQVFTNLLDNALKHTPAGGNVALSAAAVPGGVEI
                     AVTDTGPGIPSEDLQRVFERFYQVDKSRVRPAGGGVGLGLTISKEIVEAHGGDIRVES
                     VVGLGTKFIVRLPPAQPDDSTVVKRRSKTPLADADRSIVARKRN"
     gene            complement(7085..7330)
                     /locus_tag="AC16CX_07820"
     CDS             complement(7085..7330)
                     /locus_tag="AC16CX_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR3012454.1"
                     /translation="MNKMMGFLAGIFCGAVVGSVAALLLAPMSGRELQSQARERFDDL
                     FDEAREAAEARRAQLEAQLAALKTRGASGEASAPAQN"
     gene            7457..>7685
                     /locus_tag="AC16CX_07825"
     CDS             7457..>7685
                     /locus_tag="AC16CX_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012455.1"
                     /translation="MSLPQNPEELMRWAWPQIEPHYQALAARDLTPANVHPWLADWSR
                     LSEHISEMKHRLSVATTVSTADADAERRYKTY"
BASE COUNT         1573 a         2324 c         2294 g         1494 t
ORIGIN      
        1 agagaaaagc aatggctaat ctcccctcag ggaccgttac attcttattt accgacatcg
       61 aaggctcgac gcgactcctg caacagttgg gcgagaaatt cgcgaccctg atcacggagc
      121 aacagcagat tttgcgcgag gcctgtgaga ctcacaatgg gcgcgtagtg agtattcaag
      181 gcgattcatt cttcatggcc tttccgagtg cggtggatgc aatcaacgcc gtggtgcaat
      241 cccaacgtga gctcgctgct cacaggtgga caaatggagt cagcgtacgc gtgcgcatgg
      301 gcttgcacac aggcgagtca caaatcaatg cgtcagatta cgtaggcatc gatgtacatc
      361 gcgccgcacg tatcgcagcc gccgcgcatg gcggacaggt gctgctctcg caaacaacac
      421 tcgatttgat ccaaaatgac ctgcccgatg aggtgacaac acgtgacctg ggcaatcatc
      481 gcttaaaaga tttgcgccag cccaaacatc tttatcaact cgtcattaat gatttgccct
      541 caagttttcc gccaatcaaa tcgctcaacg cgctggcaaa taatctgccg atacagttga
      601 cgagtttcat cggacgtgaa aaagaaataa gaggggtaaa gcaagtcatc ctcgaccatc
      661 gcctggtaac gctgacaggt ctaggtggca gcggcaagac tcgcctggcg ctgcaagctg
      721 ctgctgagat gatcgagcat tttcataacg gtgtgttttt cgtcgctttg gcgccgatca
      781 ccgatcccgg gctggttgct tcgacaatcg ctcaagcgat aggcattact gagagcggtg
      841 ggcagtctat tattgacggt ctaaaggatt atttgcaggg caaatctttg ttgctattgc
      901 tcgacaactt tgagcaagtg atttccgcgg ctgctcttgt tgccgaactc ttggtggcct
      961 gccgggaatt aaaggtttta gtcaccagcc gcgaaggact acgtatcggt ggcgagcacg
     1021 agtaccccgt gccgccgctg gcgttgccaa ttctcacaca gctgccctct ttggaagccc
     1081 ttacacaata tgctgctgtc aagttgttca ttcagcgcgc gcaggcggtc aagccggact
     1141 ttaatattac gaacgataca gcaccgacgg tggccgaaat ctgctatcgg ttggatggat
     1201 tgccgctggc aatcgagtta gcggcggcgc gcatcaagtt gttgtcgcca cgcgccatgc
     1261 tcgcccgcct ggaaaaccgt ctggaatttc tgacgggcgg tgggcgcgat ttgccagcgc
     1321 gccagcagac cctgcgcaat gccattgcgt ggagttatga tctgctaagt gaagaggaac
     1381 agaaattgtt ccggcggctg tccgtttttg tcggcggttc tacgttagag gcggtcgagg
     1441 cggtcgaggc ggtcgccggg gatcaatcag cccgcgtctc cgtattggat caattgggat
     1501 cgctcttgga caagagtttg ttgcgagagg tggaagggac ggatggcgag ccgcgttttg
     1561 tgatgttgga gctgttgcgg gagtttggct tggaacaatt ggaagggagt ggcgagcaag
     1621 aagcgatccg aggccgtcat gctaactttt gcctggcgct cgccgagggt gccgaagcaa
     1681 gcttagagag cgcggagcag gttcaatgga tggatcgcat ggagcaagag cacgacaacc
     1741 tgcgcgcagc gctcgagtgg agcaagacgg cagatggcac tggagaatta tgtttgcgat
     1801 tagctggggc gctcggatcc ttctgggaag tgcgtggcca ttttagcgag gggcgcgagc
     1861 ggctgtctgc tatactatcc acgaaatctg cacagggaca aacagcggcg cgtgccaggt
     1921 tgcttgcccg ggccgcagaa cttgcctatc ggcaaagcgt atatcctgcg actacctcac
     1981 tcgccaaaga gagtctggca atttaccgca aaattggaga tgagcagggt atcgcatcaa
     2041 cgctcattaa gttgggaaat gcagcgacag agagaggcag ttacgcaacc gcgtctcgct
     2101 ttttggagga ggcgctgacg atttggcgcg agcgaaagga caaacatggt acggctcgcg
     2161 cgttgatcag cttagggtgg gctgcactgc gttcaggaga cactcatctc gcaaacacgc
     2221 gtttggagga agcattagcc ttatcacgcg aattgggaga tgcgcggagt atgggcttcg
     2281 agctttctgg cttgggggaa gtggcgctgc gacagggtga ttacgcccgt gcgacacaac
     2341 tcatggagga gagcctggaa ctgcgcaggc agcttggtaa caaatggggt gttggtgttt
     2401 cgctcggtat gttaggctgg gttgccatgc gcgagcgaga ttgggatcgt gcaactgcac
     2461 ggctgggcga gagtctgcaa gtccgacagg aaattgggga caagggcggc agcgcctggt
     2521 gcctcgagcg cttggcaggg atcgctatcg tacagggcca ggctgagaaa gcagtgcgcc
     2581 tttttagcgc cgcagccgcg ctacgtgcct cgattggctc ggtgattgac cccgtggatc
     2641 gagcgagcta tgaaagaaat cgcagatcgc tacgtgcgaa attaggccag gagcgattta
     2701 aggccgcctg ggatgaaggc tgcgccatga aaatggagca ggctatcgcg tatgcgcttg
     2761 ggacgagcga cgcgcaataa ttttctcttt agctagcacc aggtcccaac cagcgaaaac
     2821 ctcacattcg gtatgggtag attgggtcga cttttacgga caaacgagga cagttgatgt
     2881 gcacatcgcc cacctgcgca aaatcctcgc aggcggccag gtgagcatcg agacggtgac
     2941 gggcgtggga tacaaactcg tggtggcgta aatgtctgag aagccaaaga agccttttga
     3001 cattgatctg gccatcgagc gcatccgggc ggcagtccgg cccttcccga aggcggcgat
     3061 gttcgagcta gccgaggagg gctttgcctc gccttttgaa ctgatcgtgg cttgcgtaat
     3121 atcgattcga acttatgacg aggtcacgct gcccacagcc cttaagcttt ttgcacaagc
     3181 ccggacaccg gcagccatct gccagctgac ggtcgcacaa ctggatgagc taatccgagc
     3241 cagctctttt catgaacgca aggcccagca gattcatcaa atcgcgcaac gaacggtgaa
     3301 agactacgac ggccagctac cttgcgaccg ggaggttctg ctctcgcttt ctggggtcgg
     3361 accgaaatgc gcaaacctgg cgctgggcat cgcctgtaac gaaccattca ttggcgtgga
     3421 tatccacgtc caccgtgtga cgaaccgttg gggttacgtg gccgccacga ctcccgagcg
     3481 caccctggcc gtcctcgaaa caaaattgcc acgtgcctac tgggttgaaa tcaaccggct
     3541 gctcgtcccc ttcggtaagc acatctgcac tggaaccatg ccccgctgct caacctgccc
     3601 cttgctcgat atgtgccaac aggtgggcgt taaggcccag cgttagacgc agcccacacc
     3661 actctggaga aacagaaata tcagcaccgg gttggcgcgt tgttaagcgg cctgcgccag
     3721 cccatcggcc tccggcgttt cgagtcggag cgccgcaggc agccccgcgg cccaagaccg
     3781 gatcgccgtc caattgcgcc ggtctccggc ctgagcctgg atgatcacca tgacgaacaa
     3841 caccgcccac cacttcaaac ggccatattc cagccggccg ccgaaaaacc caatgcttgc
     3901 cggtttgacc ggacgggtgg ccgtgaggat gggttgaata tagctcgaaa gcctggcata
     3961 gcgttccctg aaagtcaggc gtccttcttt ttcaggtggt ttgggcagtt tttcgtccac
     4021 acataccgga accccgccca cactcgtttc gcccgtctgc gtcaggctca tccccatgac
     4081 gaataccgcc agggggatgc gctgaaacgc tccccggtgt ttcttcagga atcccagagc
     4141 ggcgcgatgc cagcccatga tcatgggacc tccgaccacc acgccgtcgt aggcctcgag
     4201 gtccttgacc tcgccgaggg gcaggacatc cacctgcata ccgctcttgg cgatctcttc
     4261 accaacgacc cgggcgacat ccacggtcga accggccatt gtggcatagg caacaaggat
     4321 tctcttcata agaagttgtc ctgagaaaag gctaccgggt caagccccgg cctggcttga
     4381 ctgcgtttta gttgacactc agaaaaccca gcgagcgcca caacacccgc atcatagcgt
     4441 gaccgaaatc cggattcagg aattgattcg gacaggcgcc ggggatcacc gtaatgccgt
     4501 tctcgcggca catctgcgcc gcgccctgtg agacgctggt catgcccgcc gccaggccgg
     4561 gcttcgtccc catcatgcag tgcatccaga cgcgcctgat accgagatcc acgcactgct
     4621 ggacgatttg ctcggtgact ttgggattgg cgagaatgaa aaccgcatcc ggcttctcgg
     4681 gaatagacct caggtccgga tagcacgggt caccctcaaa ggtcgtcaga tgcggattga
     4741 ccgcgctgac ggcatagccc gcccccttga acttacggta acccaggttg cagcccgtct
     4801 cgcgcttatc cgaaacgccg accacggcga tccttttctg agcgaggaag tcttgtacga
     4861 gggtatcaat cttggacatg ggaactcctt tcttgtgtcc ctcccatagt aggtctgaat
     4921 atactcctac ggcccgccag ccgaacctga gcaaagtcat tcgcttgagg gacgaaggtc
     4981 acgccgagac gcctttgcga gaccaatgcg ctgccaggca aattccaaat cggattagat
     5041 ttgtcgtgat ctctcgatag gattagaatc cgggatcatg tgagctgcga ggtgaagact
     5101 tgtccaacgg ttcctgtctg gtaaaacacg ggttgtcgct aaaatgtgct agccggtcgg
     5161 cgtcatatcg cattgtcgac gaccagaaag ggtgagtgga aagatgtgcc gaagcatcaa
     5221 gcaacttcgt cgccccgagc agccgcctac ggacgaggaa ctccgggccg ccgcgcttca
     5281 gttcgtccgc aaggtaagtg gctaccgcgc cccatcgaag gcgaatgagg aggctttcaa
     5341 tcgggctgtt gccgagatcg cggcggcgac gagagtgttg ctcgacaacc tggtcgtcag
     5401 gtgatgaatg ccgaagcggc cagataacaa ggaatttaca tcgcctccct acaattccgg
     5461 tcatgaactc cctcagcctc cgccttttcg ccgcctttct agtcatcatc ctgctcgtca
     5521 cgctcgtcac cggcctcgcg ctcatcctca tcctgcgcaa caccccactc gtccagcggc
     5581 aggcgctcac ccgcctgaac gaagccgccc gcgccgcgct ccagcagggc ctgccgcctg
     5641 acgacccgtc ccccgaggct gccgcacaat acgtgaagca gctcggcgac acgtttgaag
     5701 tgcgcgtgct gatggcgggc agcggtggcg ccgtcatcgc cgactccgcc tctgccgcct
     5761 cgccgctcaa attgaacttc cgcctctcca ccgtggaccc ggcggcgccc ggcgcgcgca
     5821 gcgggcgcgc gcgcgatggc aatctccaaa gatgggtgta cgtcgcgcga tcggtagggt
     5881 cagggcgcgt gctggtcttc gcattacagg agccgcgctt tgcggcgctg gccttctttg
     5941 ccgagaacct cctcctgccg ttgctggagg ccggggccat cgccacgctc gtcgcgctgg
     6001 cgctcgccgt cctcattgcg cgctccatcg cgctgcccct gcaaaagatg gccgccgtcg
     6061 cgcagggcat cgcgcgcggc gaccattcgc aggctgcgcc cgtcagcggt ccggacgaag
     6121 tgcgcgcgct cggccagtcc ctcaaccaga tgtccacgca ggtgcaggtc acccagcagg
     6181 cccagcgcga cttcctcgcc aacgtctcgc acgaactcaa gaccccgctc acctccattc
     6241 aaggttttgc gcaggcgatg ctcgacggcg cggcggactc gccccaggcg attcagcgct
     6301 cggcgaacgt catctacact gaggccgacc ggctgcgccg gctcgtcgag gggctgctcg
     6361 acctcgcccg gctggacgtg ggcctccgcg cgctcagccg cgcccccctc gacttgcggc
     6421 ttgtgatcgc caacgtcgtc gagaagttcg gcctgcgcgc caaagagaaa ggcgtgacct
     6481 tgcgcggcga gctgcccccg gcgctgcccg ctatggtcgg ggacgccgac cggctggcgc
     6541 aggtgttcac caatttgctg gacaacgcgc tcaagcacac ccccgctggg ggcaacgtgg
     6601 cactgtcggc ggcggcggtt ccgggcggag tcgagatcgc cgtcacagac accggccccg
     6661 gcatcccgtc cgaggacctc cagcgtgttt tcgagcgatt ctatcaggtg gacaaatcgc
     6721 gagtgcgccc cgcaggcggg ggcgtgggac tggggctgac gatcagcaag gagatcgtcg
     6781 aggcccacgg gggggacatc cgcgtggaga gcgtcgtggg gctggggacg aaattcatcg
     6841 tgcggctgcc acccgcgcag cccgacgaca gcaccgtggt caagcgccgg agcaaaactc
     6901 cgctggccga cgccgaccgt tcgatcgtcg cccgcaagag aaattaaagg tgcgacgcac
     6961 ttctgaccct ctccgctccg ctacgagggc cttcggagtg cgtcgcacct tgctggtcag
     7021 ccgccaggcc acgccgacag cctgacaccg ccgcgcgctg acgggaatga tcggcgcgcg
     7081 ttcctcaatt ctgggcaggc gccgaggcct ccccggacgc gcctcgagtc ttgagcgcgg
     7141 cgagctgcgc ttcgagctgc gcccgcctgg cctccgccgc ctcacgcgcc tcgtcaaaca
     7201 gatcgtcgaa ccgctcgcgc gcctgagact gcaattccct cccggacatg ggagccagca
     7261 gcagcgccgc caccgaaccg accaccgcgc cgcaaaatat acctgcgaga aatcccatca
     7321 ttttgttcat gggtgagcct ccttctctaa gctgaacccg ccaaatcatt atatgctacg
     7381 cggtacgggt cgtaaaggtt tcgcgctata atgcccgctg gcacgacgct agccccatcc
     7441 ccccgaggga gaaaaaatgt ctctgcctca gaatcccgaa gaactcatgc gctgggcgtg
     7501 gccgcagatc gagccgcact atcaggccct cgccgcgcgc gacctcaccc ccgcaaatgt
     7561 ccatccatgg ctcgcggatt ggtcgcgcct gagcgagcac atctctgaaa tgaagcaccg
     7621 cctgtccgtg gcgacgacgg tcagcaccgc cgacgcggac gccgaacggc gctacaagac
     7681 ctacc
//
LOCUS       DBNGWZ010000262         2483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3176531, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000262 DBNGWZ010000000
VERSION     DBNGWZ010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3176531"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC16CX_07830"
     CDS             complement(<1..389)
                     /locus_tag="AC16CX_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012456.1"
                     /translation="MTTRIFSFGLWTVMNRGADPFGAPTRAPLDALQAISGLAAHGVR
                     AFELHAEDLIPPGGALGERDRLIQEARRRMADTGIVCDACGSSLFIDPIFKDGALVSN
                     EARVRRMAIARYQEAIDVGHLLDAPLF"
     gene            489..1664
                     /gene="galK"
                     /locus_tag="AC16CX_07835"
     CDS             489..1664
                     /gene="galK"
                     /locus_tag="AC16CX_07835"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR3012457.1"
                     /translation="MEHGSALQESVVRLFTERFGGEPALLAQAPGRVNLIGEHTDYND
                     GLVLPAAIDRHVVIAARPNSDGRIHLVSSAYRERAEFPAADPESPTLPLWARYTQGVA
                     VKMAARGIPLAGLDGAVAADLPIGAGLSSSAALEVASALVLEAVAGQALPPRERALLC
                     RTAEVEFVGVPCGIMDQFAVTLCRRGHALFLDCRSLETHHIPLGPDLAIAVCDTGVAR
                     TLAGSAYAQRRSECEQAVAALQAAGMRIRALRDIAPDDLPAVERLPDPMRRRARHIVT
                     EDARVVETARRLEGNESAGLRDVFLASHRSLRDDYEVSSPELDAMVDAALESPGCIAA
                     RMTGGGFGGAAVALVRRTQSEGFLAAAATGYRKRTGREGKFFLTEAAEGARMRQQRD"
     gene            1816..2064
                     /locus_tag="AC16CX_07840"
     CDS             1816..2064
                     /locus_tag="AC16CX_07840"
                     /inference="COORDINATES: protein motif:HMM:NF021253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HIR3012458.1"
                     /translation="MGYRRAIGSVAYKLASEALKLSPRQRARLAERLIASLDREREPG
                     SATLGAKEAQRRLAELRTGKAKARPVAAVFRKARAPLR"
BASE COUNT          440 a          794 c          853 g          396 t
ORIGIN      
        1 ttgaagagcg gcgcatccag aagatgccca acgtcgatgg cttcctggta gcgggcgatc
       61 gccatccggc gcacccgtgc ttcgttagag acgagggcac cgtctttgaa aatcgggtcg
      121 atgaataacg aagacccgca ggcgtcacag acgatcccgg tgtccgccat tcgccggcgg
      181 gcctcctgga tcagtcggtc ccgttccccc agggcgccgc cgggcggaat gaggtcctcg
      241 gcgtgcagtt cgaaggcccg cacgccgtgc gcggccaggc cgctgattgc ctgcagcgcg
      301 tcaagcgggg cgcgggtagg agcgccgaag ggatcggctc ccctgttcat caccgtccac
      361 agcccaaagc tgaagatccg cgtggtcatc gcctgtgatc cgcctccctc atctgcatct
      421 tctgatcacg ctcacgggcc ccttcagcgc aggacgggag ccggacagcg agaaggcaga
      481 aggcgcgcat ggagcacgga tccgcgctgc aggaatcagt ggtgcgcctc ttcaccgagc
      541 gatttggcgg tgagccggcg ctcctcgccc aggctcccgg gcgggtcaac ctcatcggcg
      601 agcacaccga ctacaacgat ggcttggtgc tgccggcggc gatcgaccgg cacgtggtca
      661 ttgctgcacg gccgaacagc gatggccgga tccacctggt cagttcggcg tacagagagc
      721 gggccgagtt ccccgcagcc gatcctgaat cacctacgct tccgctgtgg gccagataca
      781 cacagggcgt cgcggtgaag atggccgcgc ggggaatccc gctggccggt ctggatggag
      841 ccgttgcggc ggatctgccg atcggcgcgg ggctcagctc gtctgcggcg ctagaagtcg
      901 cgtcggccct cgtgttggag gcagtcgccg ggcaggcgct gccgccccgc gagcgcgcgc
      961 tgctgtgccg gaccgcggaa gtggaattcg tgggcgtacc gtgcggcatc atggaccagt
     1021 ttgccgtgac gctgtgccgg cgggggcacg cgctgtttct ggactgccgg tcactggaaa
     1081 cgcaccacat cccgctgggt ccggatctgg cgatcgccgt ctgcgacacc ggagtcgcgc
     1141 gcacgctggc gggatcggcg tacgcgcagc ggcggtccga atgcgaacag gccgtcgcgg
     1201 cgctgcaggc ggccgggatg cggatccgag cgcttcggga catcgccccc gatgacctcc
     1261 ccgctgtcga gcggctgccc gacccgatgc ggcggcgggc gcggcatatc gtcaccgaag
     1321 atgcccgcgt cgtcgagact gcgcgtcggc tggagggcaa cgagtctgcc ggattgcggg
     1381 acgtgttcct cgcctcacac cgaagcctgc gcgatgatta cgaagttagc agccctgagc
     1441 tcgatgcgat ggtagacgcg gccctggagt cgccgggctg catcgccgcg cgcatgacag
     1501 gcggaggctt cggcggcgca gccgtggcgc ttgtgcggcg aacccagagc gagggcttcc
     1561 tcgccgccgc agccacagga taccgtaagc gaaccggccg cgaaggcaag ttcttcctca
     1621 ccgaggcggc agagggcgcc cgaatgcgac aacagcggga ttagggattc gggattaggg
     1681 aatggcgcct caacgtcctc tcgagtccca cagtcccgat cactgatccc gcagtcatcc
     1741 tgctcagacc aagaacccgc cgcaacgctc gactcgagat tggtgggcga cccccctgta
     1801 gtcaggcgcg gtgatgtggg gtaccgtcga gccataggca gcgtagccta caaactcgcg
     1861 tctgaggcct tgaaactttc tccccggcag cgtgcgcggt tagcagagcg cctgatcgcc
     1921 agccttgatc gggagcgtga gcccggaagt gcgactctgg gggcgaaaga ggcccaacga
     1981 cgcctcgcag aacttcgaac tggaaaggcg aaggctaggc cagtagcagc ggtttttcgg
     2041 aaggcccgcg caccgcttcg gtgagaaatg ccgccttccc cccggaggcc gaggaagaat
     2101 tcattgccgc tgccgggttc tacgaaaagc acgccggagg ccttggccgg ttcatcgcag
     2161 aagttcggca caccgtccgg cgcatcgttg cccatccgga aagcggtgtc tggttctcaa
     2221 gaggccttcg acgtacaaac gcacaagaga agcgccgaaa aaggcgctgc gccggaagag
     2281 tcttcccccc cgcttcaccc ccgccagtga gcgctggcgt tagacggcag attgacggcg
     2341 cgacagagcc ctgggacgat tgctgagtgg acttcacgat cgtcggcgac attacggaca
     2401 tcgagacatt cgccgttggg aaagcgattc gcgagattgg caggctgcga agacgatatg
     2461 gacgtgggcg gtggcggaag cgc
//
LOCUS       DBNGWZ010000263         5353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2546649, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000263 DBNGWZ010000000
VERSION     DBNGWZ010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2546649"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="AC16CX_07845"
     CDS             <1..720
                     /locus_tag="AC16CX_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012459.1"
                     /translation="PDGYTMLLGNLTPVAINPHMYRKIGYDTLRDFAPVSLVAAAPQL
                     VVVNPAVPAKSVKEFIALAKAKPGQLNYGSGGLGTLAHLGAEMFKLQAGVNMVHVPYK
                     GTVLSVADLVAGQVQVVFSDMPPALPHARSGKLRALAVTGAKPSALAPGVPTVAETVP
                     GFLLENWWGVVVPKGVPGPIVTKINAEIRRALEQADVKERYANVGIEPVTSTPQQLGD
                     QIRSESAKYAKLIKDAGIKAE"
     gene            747..1724
                     /locus_tag="AC16CX_07850"
     CDS             747..1724
                     /locus_tag="AC16CX_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012460.1"
                     /translation="MRSQAAYWSIALGATLLVGMAPLAAQQYPSRPVRFVVPFAPGGS
                     TDTLSRTIGVKLADALGHQVVVDNRPGGNGDIGMMIVAKAPPDGHTIVLGYIANLAIA
                     PSLYAKMPYDPVKDYAPITQPASSPNVLTAHPSVQAKNLQELIALAKAKPGQVSFAST
                     GVASVGHLTGELINNLAGIKMTHVPYKGSGQAVTDILGGHVQVMFSGFSSTLHHIKAG
                     KLRALAVTGPKRSDALPEVRTIAEQGFPGVEATAWYGILAPAGTPRPVVARLHGEIVK
                     ILKLPDVTQKLEGLGFEIVGSTPEQLGAYIKSEIRKWEKVVKASGAKPD"
     gene            complement(1887..4136)
                     /gene="parC"
                     /locus_tag="AC16CX_07855"
     CDS             complement(1887..4136)
                     /gene="parC"
                     /locus_tag="AC16CX_07855"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HIR3012461.1"
                     /translation="MALFAERSYLTYAMSVVRGRAIPNVEDGQKPVQRRILYAMNELG
                     LKAGVQHSKSARIVGEVLGKFHPHGDASTYEALVRMAQDFSLRYPLIDGQGNFGTQDG
                     DPAAAYRYTEARLTPLAELLLSEIDLDTVDFIPNYDGRLKEPKLLPARLPMLLLNGAS
                     GVGVGMACELPPHNMKEVAEAAVFMVKHPNATPKALMHIIKGPDFSGGGRIISPREEI
                     FEAYKTGRGSLRARARWKVENLARGQWQIVVHELPPTTSVKRVMAEIEAITNPKPKEK
                     GGKISAAQANLKQVMLNQLEKVNDESDKENKLRLVLEPRSSRQDPDEFMRFMLTHTSL
                     EENFPVNLVALGLDGRPTRKNIAELVGEWVQFRIATATRRSKYRLSQVEKRVHILEGR
                     VIVLLNIEKVIKVIRNSDEPKADLMKQFKLTEAQAEDILEIRLRQLARLEGIRIETEL
                     KELKDERKALRGLLGSDEEMKKLVAEEIRNDAKTYSDARRTQIEAAERATFERQVIDE
                     PLTIIVSRNGWVRARAGHGLDLSGTTYKAGDGPYLAFETRSVHSVGIIDSTGRAFSVE
                     CSELPSGKGDGQPLASFIEIAPGARLAHVVDAQPGKRYLVANSGGYGFIVKSEEFLTR
                     VKAGKVFMTLEEGEEVIRPALVPDKPELAAAFSENGRMLLFPAEELKEIARGRGITLM
                     GLDEGEKLVAVGFANSQSVTVNGASRSGKEKTVKVGGADLQKHILHRARKGCLLPGKL
                     KPTGVVAEG"
     gene            complement(4388..>5353)
                     /locus_tag="AC16CX_07860"
     CDS             complement(4388..>5353)
                     /locus_tag="AC16CX_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR3012462.1"
                     /translation="SAKVLEPQFHGQTKEKLSNRDAVKLVSTMVRDPFAAWMTANEEH
                     GKKICDLVIRQAIERARTVQKVERKRSSGVVLLPEKLSDCESTDLNQNELFLVEGDSA
                     GGSAKQARDKRFQAIYRMRGKALNSWEVKRELLFSNAEIHDIAVALGVDPHDEKDSPD
                     LSGLRYGKIASMTDADVDGAHISVLLLTLFYRHFPKLIEKGHVFIACPPLYKVDIPGH
                     GKRAPKKLYALDEDELKSILDRARDDGVKRESIMIQRFKGLGEMNPGQLWETALNPDT
                     RKLLKVRIEDRNQTFRLFNMLMSKKEAEKRCAWMEEKGNTVEADV"
BASE COUNT          829 a         1784 c         1753 g          987 t
ORIGIN      
        1 cccgatggct acaccatgct gctcggcaac ctgacaccgg tcgcgatcaa ccctcacatg
       61 taccgcaaga tcggatacga cacgttgcgc gattttgcgc cggtgtcatt ggtcgccgcg
      121 gcgccgcaac tggtagtggt aaaccccgcc gtgcccgcga aatcggtcaa ggagttcatc
      181 gcgctggcga aagcgaaacc gggccagctc aattacggct cgggcggcct gggaacgctg
      241 gcgcacctcg gtgccgagat gttcaagctt caggctggcg tcaacatggt ccacgtcccg
      301 tataagggca ccgtgctttc cgtcgccgac ctcgttgccg ggcaggtgca ggtggtgttc
      361 tcggacatgc cgccggccct gccgcatgca aggagcggaa agctgcgggc gctcgcggtg
      421 accggggcaa agccgagcgc gcttgcaccc ggcgtgccca cggttgcgga aacggtcccc
      481 ggattcctgc tcgagaactg gtggggggtc gtggtgccga agggcgtgcc cggccccata
      541 gtgacgaaaa tcaacgccga gatccgacgc gcgctggagc aggccgacgt gaaggagcgc
      601 tatgccaacg tcgggatcga acccgtcacg agcacgccgc agcagctcgg cgatcagatc
      661 aggagcgaat cggcgaagta cgcgaagctc atcaaggacg ccggcatcaa ggcggaatga
      721 tgcgggtgag gggtgaggag agaggggtga ggagtcaagc cgcttactgg agcatcgcgc
      781 tgggcgcgac gctcctggtt ggtatggcgc cgctcgccgc acagcaatac ccctcgcggc
      841 cggtgcgttt cgtggtgccg ttcgcccccg gtggcagcac cgacacgctc tcgcgcacca
      901 tcggcgtgaa gctcgccgac gcgctcgggc accaggtggt ggtggacaac cgcccgggcg
      961 gcaatggcga tatcggcatg atgatcgtcg ccaaggcgcc gcccgacggc cacaccatcg
     1021 tgctcggcta catcgccaac ctcgcgatcg cgccgagcct ctacgcgaaa atgccgtacg
     1081 acccggtgaa ggactatgcg cccatcaccc agccggcttc ctcgcccaac gtcctgacgg
     1141 cgcatccctc ggtgcaggcg aagaacctgc aggagctgat cgcgctcgcg aaagcgaagc
     1201 ccgggcaggt cagtttcgcc tcgactgggg tcgccagcgt ggggcatctc accggcgagc
     1261 tgatcaacaa cctggccggg atcaaaatga cgcatgtgcc gtacaagggc agcggccagg
     1321 cggtgaccga catcctgggc gggcacgtgc aggtgatgtt cagcggcttt tcctcgacgc
     1381 tgcatcacat caaggccggg aagctgcgcg cgctggcggt gaccggcccc aagcgctccg
     1441 acgcgctgcc ggaggtgcgg accatcgcgg agcagggctt cccaggcgtc gaggcgacgg
     1501 cgtggtacgg catccttgcg cccgccggca cccccaggcc cgtcgtcgca cggctgcacg
     1561 gcgagatcgt caagatcctc aagctccccg acgttaccca gaagctggaa gggctgggct
     1621 tcgagatcgt cggctcgacg ccggagcagc tcggcgccta tatcaagtcc gagatcagga
     1681 aatgggagaa agtggtcaag gcctcgggcg cgaaacccga ttagcgggtg atgagtgatg
     1741 ggtaatgggt gatgagagtt cccggcggac gccgggggtc atccttggtc agtgaccccg
     1801 gcccgccgcg ctgcatcgcg tcgggcgttc gccggctcga acgtgcgcag gcacgttctt
     1861 cgaattcctg gctcgcccct catccttcat ccttcggcaa ccacgcccgt cggcttcaac
     1921 ttgcccggga ggagacagcc ctttctggcc cggtgcaaaa tgtgcttctg caggtcggcg
     1981 ccgcccacct tcacggtttt ctccttgccc gagcgcgatg cgccgttgac ggtgaccgat
     2041 tgggaattgg cgaagccgac cgcgacgagc ttctcgccct cgtcgagtcc catcagcgtg
     2101 atgccgcggc cgcgcgcgat ctccttcaac tcctccgcgg ggaacagcag catgcggccg
     2161 ttctcggaga atgcggccgc cagctccggc ttgtccggca cgagggcggg gcggatcact
     2221 tcctcgcctt cctccagcgt catgaaaacc ttgcctgcct tcacgcgcgt caggaactcc
     2281 tcggatttca cgatgaagcc gtagccgccg gagttggcga cgaggtagcg cttgccgggc
     2341 tgcgcgtcca ccacgtgcgc gagccgcgcg ccgggcgcga tctcgatgaa cgaggcgagc
     2401 ggctggccgt cgcccttgcc gcttggcagc tccgagcact ccacgctgaa ggcgcggccg
     2461 gtggagtcga tgatcccgac gctgtgcacc gagcgcgtct cgaaagccag gtacggtccg
     2521 tcgccggcct tgtaggtggt acccgaaagg tcgagcccgt ggccggcgcg cgcccgcacc
     2581 cagccgttcc tcgagacgat gatggtgagc ggctcgtcga tcacctggcg ctcgaaggtc
     2641 gcgcgctccg ccgcctcaat ctgggtgcgg cgcgcgtcac tgtaggtttt cgcgtcgttg
     2701 cggatctcct cggccaccag cttcttcatc tcctcgtccg agccgagcag gccgcgcaat
     2761 gccttgcgct cgtccttcag ctccttcagc tcggtctcga tccggatgcc ctcgaggcgc
     2821 gcgagctgcc gcaggcggat ctccaggatg tcctcggcct gcgcctcggt cagcttgaac
     2881 tgcttcatca ggtcggcctt gggctcgtcc gagttgcgga tgaccttgat taccttttcg
     2941 atattgagca ggacgatgac gcggccttcg agaatgtgaa cgcgcttctc gacctgcgag
     3001 aggcgatact tcgagcgccg cgtggcggtg gcgatgcgga actgcaccca ctcgccgacg
     3061 agttccgcga tgttcttgcg ggtgggccgg ccgtcgaggc ccagcgccac taggttcacc
     3121 gggaagttct cctccaggct cgtgtgcgtc agcatgaagc gcatgaactc gtccgggtcc
     3181 tggcgcgagg agcgtggctc gagcacgagg cgcagcttgt tctccttgtc cgactcgtcg
     3241 ttgaccttct cgagctggtt caacatcacc tgcttgagat tggcctgcgc cgccgagatc
     3301 ttgccgcctt tctccttggg cttggggttg gtgatggcct cgatctcggc catgacccgc
     3361 ttcaccgagg tcgtcggcgg cagctcgtgc accacgatct gccactggcc gcgcgccagg
     3421 ttctccacct tccagcgcgc ccgcgcgcgc agcgacccgc gcccggtctt gtaggcttcg
     3481 aagatctcct cgcgcgggct gatgattctc ccgccgccgg agaagtccgg ccccttgatg
     3541 atgtgcatca gggccttggg cgtggcgttg gggtgcttca ccatgaacac cgcggcctcc
     3601 gccacttcct tcatgttgtg cggcggcagc tcgcacgcca tgccgacgcc gaccccggag
     3661 gcgccgttga gaagcagcat cggcagccgc gccggcagga gcttgggctc cttcagccgc
     3721 ccgtcgtaat tggggatgaa atcgaccgtg tccaggtcga tctccgagag cagcaattcg
     3781 gccaacggcg tgaggcgcgc ctcggtatag cggtacgccg cggcgggatc tccgtcctgc
     3841 gtgccgaagt tgccctggcc gtcgatcagg gggtagcgca gcgagaaatc ctgcgccatg
     3901 cgcaccagcg cctcgtaggt ggaggcgtcg ccgtgcggat ggaacttgcc gagcacctcg
     3961 ccgacgatac gcgcggactt tgaatgctgg accccggcct tcaggcccag ctcgttcatg
     4021 gcgtagagga tgcggcgctg caccggcttc tggccgtcct ccacgttggg gatcgcccgg
     4081 ccccgcacca cgctcatcgc gtacgtcagg taactgcgct cggcgaacaa tgccatgtcg
     4141 atggaatcgt cgcccgacag taccggcccg ccgcccttgc cgccgccctt gccgccgcgg
     4201 tcggcatcgg ccgcctggcc gcgcgccttc ttctccttcc gggccggctt gctccgggtc
     4261 cggacttttt ccgctttcgt cgccatcggg gtgtccacct tctctgcgac tgccggttcc
     4321 gcggcctgct cgaaaaggtc cggcgtcgcc tcgtcagctt cgcgcttcgc gcgaatccgg
     4381 gtatcggtca tacgtcagcc tcgaccgtgt tacctttttc ttccatccac gcgcagcgct
     4441 tctccgcttc cttcttcgac atcagcatat tgaagagccg gaaggtctgg ttgcggtcct
     4501 cgatgcgcac cttgagcagc ttgcgcgtgt cgggattgag cgcggtctcc caaagctggc
     4561 cgggattcat ctcgcccagg cccttgaagc gctggatcat gatcgactca cgcttcacgc
     4621 cgtcgtcgcg cgcgcggtcg aggatcgact tcagctcgtc ctcgtcgagc gcgtagagct
     4681 tcttcggcgc gcgcttgccg tggccgggaa tgtccacctt gtaaagcggc gggcaggcga
     4741 tgaagacgtg gcctttctcg atcagcttcg ggaagtggcg gtagaagagg gtgaggagca
     4801 gcaccgagat gtgcgcgccg tccacatccg catcggtcat cgaggcgatc ttgccgtagc
     4861 gcaggcccga gagatcgggg ctgtccttct catcgtgggg gtccacgccg agcgcgaccg
     4921 cgatgtcgtg gatctcggcg ttggagaaca ggagctcgcg cttcacttcc caggaattga
     4981 gcgccttgcc gcgcatgcgg tagatcgcct ggaagcgctt gtcgcgcgcc tgcttggccg
     5041 atccgcccgc cgagtcgcct tcgaccagga acagctcgtt ctggttgagg tcggtggact
     5101 cgcagtcgga gagcttctcg gggaggagca ccacgcccga ggagcgcttg cgctcgacct
     5161 tctgcaccgt gcgcgctcgt tcgatcgcct gccggatgac gaggtcgcag atcttcttgc
     5221 catgctcctc gttggcggtc atccacgccg cgaacgggtc gcgcaccatt gtcgacacca
     5281 gcttcaccgc gtcgcggttg gagagcttct ccttggtctg cccgtggaac tggggctcga
     5341 gcaccttggc cga
//
LOCUS       DBNGWZ010000264         2000 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1035917, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000264 DBNGWZ010000000
VERSION     DBNGWZ010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1035917"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="AC16CX_07865"
     CDS             <1..1133
                     /locus_tag="AC16CX_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3012463.1"
                     /translation="DEIARAPDAQLPPPAGADDTVLIIYTSGTTGFPKGAMHSQRNFV
                     TAGESNISRLWLQPDERMMVVLPMFHVNALFYSTAGALAAGCALVLMPRFSASDFWNA
                     AVECGVTQINIIEAIGRILARRPRSEFRPEHRITKVYGMRADIVAPFRDEFRIPHLLG
                     GFGMTEIPGVCCNLFEGPNKEGSMGPVGRHPDPNRKWAECKVVDDNGDEVGAGVVGEF
                     WVRHPIVMQGYFRDPEQTKTAFRDGWFMTGDLVTRDADGYYFFVSRKKDIIRRRGENI
                     AGAEIDRVIQSHPGVFEVAAVPVPSELGEDEILVAIVPKPGATLTPQEIAHWCSERLT
                     AMKVPRFVLFVNDLPHTPTHKVAKQVLKSDKTLRARAVDLQG"
     gene            1230..>2000
                     /locus_tag="AC16CX_07870"
     CDS             1230..>2000
                     /locus_tag="AC16CX_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012464.1"
                     /translation="MQSILLAGALLVAPSAFAQQASGQAFPDKPILLYNAYSTGGTSS
                     LTMRALADAASKSLGKRVIVEDKPGAGGALAPAIMSKAKPDGYTLSQMPQPMLRIPHI
                     QNVSYDALKDFTWIIRVVDYTYGVVVKADAPWKTWNEFIAYAKANPGKITYGTSGIGT
                     TMHVAMEEVAALERIKWVQVTYKGVPEMNSAVLGGHIDIVASSTGWAEMVDAGRLRIL
                     AAFGANRIKRWPNVPTLKELGYNVVASSSYGIGGPKDMD"
BASE COUNT          379 a          611 c          650 g          360 t
ORIGIN      
        1 tcgacgagat cgcgcgcgct cccgatgcgc agctgccacc gccggccggc gccgacgaca
       61 ccgtactcat catctacacc tcgggcacga ccggctttcc caagggcgcg atgcacagcc
      121 agcgcaactt cgtcacggcg ggcgagtcga acatctcgcg attgtggctg cagccggacg
      181 agcgcatgat ggtggtgctg ccgatgttcc acgtaaacgc gctgttctat tcgacggcag
      241 gggcgctcgc cgcgggctgc gcgctggtgc tgatgccgcg cttctccgcg tccgatttct
      301 ggaacgccgc ggtcgaatgc ggcgtgaccc agatcaacat catcgaggcg atcgggcgca
      361 tcctcgcgcg ccggccgcgc tcggagttcc gtcccgagca caggatcacc aaggtctacg
      421 gcatgcgcgc cgatatcgtc gcgccgttcc gggacgagtt tcgcattccg catctgctcg
      481 ggggcttcgg catgaccgag attcccggcg tgtgctgcaa tctgttcgag gggcctaaca
      541 aggagggcag catgggtccg gtcgggcgtc accccgatcc gaaccgcaag tgggcggagt
      601 gcaaggtcgt cgacgacaac ggcgacgagg tcggcgccgg cgtcgtcggt gaattctggg
      661 tcaggcatcc gatcgtcatg cagggctatt tccgtgaccc ggaacagacg aagacggcgt
      721 ttcgcgacgg ctggttcatg accggggacc tggtcacccg cgatgccgac ggctattact
      781 tctttgtctc gcgcaagaag gacatcatcc gccgccgcgg cgagaacatc gccggcgccg
      841 agatcgaccg cgtgatccag tcgcatcccg gcgtgttcga ggtggcggcg gtgccggtgc
      901 cctcggagct gggcgaggac gagatcctgg tcgcgatcgt gcccaagccg ggcgcgacgc
      961 tcacgccgca ggaaatagcg cactggtgct cggagcgcct gaccgcgatg aaggtgccgc
     1021 gcttcgtatt gttcgtcaac gacttgccac atacgcccac gcacaaggtc gccaagcagg
     1081 tgctgaagtc cgacaagacg ctcagggcac gggccgtgga tttacagggc tagggagtgg
     1141 tacggtcccg gcggcactgt ggcagaatca ggcgcaaaac gccggttact cgagttggga
     1201 ggctaccgat gactgcgcat ttgtcgacaa tgcagagcat cttgctggct ggagcccttc
     1261 tggtggcgcc atcagccttc gcgcagcaag cttccgggca agccttcccg gataaaccta
     1321 tcctcctgta caacgcgtat tccaccggtg gcactagcag cctgacgatg cgcgcattgg
     1381 ccgatgccgc ctcgaaaagt ctcggcaagc gcgtcattgt cgaagacaag cccggcgctg
     1441 gcggagcatt ggcgcccgcc atcatgtcca aggcgaaacc tgacggatac acgctgtcgc
     1501 agatgccgca accgatgctg cgcatcccgc acatccagaa tgtcagttac gatgcgctca
     1561 aagatttcac ctggatcatt cgcgtcgttg attacaccta tggcgtggtg gtcaaggctg
     1621 atgcaccgtg gaagacgtgg aacgagttca ttgcctacgc caaggccaat cccggcaaga
     1681 tcacgtacgg gacgagcggc attggcacca cgatgcacgt ggcgatggag gaggttgcag
     1741 cgctcgaacg catcaagtgg gtacaagtga cctataaggg tgtgcccgag atgaatagtg
     1801 ccgtgcttgg cggccacatc gatattgttg cgagctcgac cggctgggcc gagatggttg
     1861 atgccggtag gttgcggatt ctggcggcat tcggcgccaa ccgcatcaaa cgctggccca
     1921 atgtgcccac attgaaggaa ttgggctaca acgtcgtggc tagctcctcc tacgggatcg
     1981 gcgggccgaa ggacatggac
//
LOCUS       DBNGWZ010000265         1854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2421711, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000265 DBNGWZ010000000
VERSION     DBNGWZ010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2421711"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="AC16CX_07875"
     CDS             <1..584
                     /locus_tag="AC16CX_07875"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3012465.1"
                     /translation="RSRIKDISRIKQIRVFAEEGAYDHLVKIRQDPWNPISRETADHS
                     LPYIVAAAVLDGAITVNSFTPKVVLNPERQAFIKKVACTPALELGSHAMGKHKRVEMG
                     YLSRVEIELDDGTVVHGDARPFPGHHKNPFTDADLNAKLRECVEPVTGTQRAARLASL
                     LWSLDKVRNTRELTRLLAFGGKIDIDSARVRER"
     gene            626..>1854
                     /locus_tag="AC16CX_07880"
     CDS             626..>1854
                     /locus_tag="AC16CX_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3012466.1"
                     /translation="MLNREMNEFLTRTGPGTPMGELLRRYWIPAMLDWELPEPDCPPV
                     RVKLLGEKLVAFRDTERRIGLIEEFCAHRGVSLWFGRNEECGLRCAYHGWKYDVTGQC
                     VDLPSEPEESGMRGRIKLKSYPCVELGGVIWAYMGPPAEQPPLPNFEWVHLPPSHRVI
                     TKRWQESNYLQAIEGGIDSSHVSFLHSGEMSTDPFHRNTGGARFVKSTRTTFEIFDSA
                     GGMIIGARRDADPGNYYWRITQYIMPWYTLIPPYANNALNGHAWVPMDDENCMAWTMT
                     FHPTAPIPEKDVELIKDGNGVHAELIPGTFRPVANKSNDYLIDRTKQKSGRHYSGVKG
                     LAIQDASVQESIGPIADRTREHLVSTDNAIIMARQRLMKAAKAVQEGGRAPGLDPDDQ
                     LIRSASFVLPANASFRER"
BASE COUNT          354 a          613 c          606 g          281 t
ORIGIN      
        1 cgcgctcgcg gatcaaggac atttccagga tcaagcagat acgcgtattc gccgaggagg
       61 gcgcctacga ccacctggtg aagatacgcc aggacccgtg gaatccgatc tcgcgcgaga
      121 cggcggatca ttcgctgccc tacatcgtcg cggcggcggt gctcgacggc gccatcacgg
      181 tgaacagctt cacgccgaag gtcgtgctca atcccgagcg gcaggcgttc atcaagaagg
      241 tcgcctgcac gcccgcgctg gagctcggct cgcatgccat gggcaagcac aagcgcgtcg
      301 agatgggcta cctgtcgcgc gtcgagatcg agctcgacga cggcacggtc gtgcacggcg
      361 acgcgcggcc gttccccggc catcacaaga atccgtttac cgacgcggac ctcaacgcca
      421 agctgcgcga gtgcgtcgag ccggttacgg ggacgcagcg cgcggcgagg ctggcgtcgc
      481 tgctgtggtc cctcgacaag gtccggaaca cgcgcgaatt gaccaggctg ctcgcgtttg
      541 gcggtaagat tgacatcgac agcgcccggg tgcgcgagcg atgaccgcgc gccgcttgaa
      601 cctcgaatcg agacggtgac ggagcatgct gaaccgagag atgaacgagt ttctcacccg
      661 caccggcccg ggcacgccga tgggcgagct cttgcggcgc tactggattc ccgcaatgct
      721 cgactgggaa ttgccggagc cggactgccc gccggtgcgc gtgaagctgc tgggggagaa
      781 gctggtcgcc ttccgcgaca cggagcgccg gatcgggctg atcgaggagt tctgcgcgca
      841 ccgcggcgtc tcactctggt tcgggcgcaa cgaggagtgc ggcctgcgct gcgcgtacca
      901 cggctggaag tacgacgtca ccgggcagtg cgtggacctg ccctcggagc cggaggaatc
      961 cggcatgcgc ggccgcatca agcttaagtc ctatccctgc gtcgagctgg gcggcgtgat
     1021 ctgggcctac atggggcctc ccgcggaaca gccgccgctg ccgaacttcg aatgggtgca
     1081 cctgcctccg tcgcaccgcg tcatcaccaa gcgctggcag gaaagcaact acctccaggc
     1141 gatcgagggc ggcatcgatt ccagccacgt ttcgttcctg cacagcggcg agatgagcac
     1201 cgacccgttc caccgcaaca ccggcggcgc gcggttcgtg aaatccacca ggaccacctt
     1261 cgaaatcttc gactcggccg gcggcatgat catcggcgcg cggcgcgacg cggatcccgg
     1321 caattactac tggcgcatta cgcaatacat catgccgtgg tacacgctga ttccgccgta
     1381 cgcgaacaac gcgctcaacg gccatgcctg ggtgccgatg gacgatgaga actgcatggc
     1441 ctggaccatg acgtttcatc ccaccgcccc catccccgaa aaggacgtcg agctcatcaa
     1501 ggacggcaac ggcgttcacg cggagctgat acccggcacc ttccggccgg tggccaacaa
     1561 gagcaacgat tacctgatcg atcgcaccaa gcagaagagc ggccgccact acagcggggt
     1621 caaggggctc gcgatccagg acgcgtcggt acaggagagc atcgggccga tcgcggaccg
     1681 gacccgggag catctcgtat cgaccgacaa cgcgatcatc atggcgcggc aacggctgat
     1741 gaaggccgcg aaggccgtgc aggaaggcgg ccgcgcgccg ggcctcgacc cggacgacca
     1801 gctcatccgc tcggcatctt tcgttcttcc cgcgaacgcc agtttcaggg agcg
//
LOCUS       DBNGWZ010000266         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1791150, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000266 DBNGWZ010000000
VERSION     DBNGWZ010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1791150"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16CX_07885"
     CDS             complement(<1..307)
                     /locus_tag="AC16CX_07885"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR3012467.1"
                     /translation="MIGILIITHGTLGESLIHSASHVLNKRPVRLKQIGITAQDDPFQ
                     LLPQARKLVKELDEGDGVLILSDMYGGSPSNIAAKLLVPGKVEGVAGVNLPMLIRVLT
                     "
     gene            complement(410..1375)
                     /gene="gshB"
                     /locus_tag="AC16CX_07890"
     CDS             complement(410..1375)
                     /gene="gshB"
                     /locus_tag="AC16CX_07890"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HIR3012468.1"
                     /translation="MKLAFVADPLDSFKIYKDTTFAIMREAAARGHNLYALEQDQLAW
                     RGGIVHGHAARLHLTGEKPCWYRVEEPASTPLKDFDAVLMRKDPPFDMEYVYSTYLLE
                     LAETQGARVFNRPRAIRDWNEKMAIARFPRFTPPAVVTRRESLIREFLAEHRDVILKP
                     LEGMGGASVFRVRHDDPNVNVIIETVTHYGRRTVMAQRYIPEIRNGDKRVLLIAGKPV
                     PYCLARIPKPGETRGNLAVGATGVARELTPRDREIAEALGPVLDKEGLLLVGLDVIGD
                     YLTEVNVTSPTCFQEITEQTGFNVAGMFMDALEAACAGREHAKAV"
     gene            complement(1575..2993)
                     /locus_tag="AC16CX_07895"
     CDS             complement(1575..2993)
                     /locus_tag="AC16CX_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3012469.1"
                     /translation="MFSVVGLNTVPVVALAQDKPAAAPAPAATPSAAPAAPAAAPATT
                     PAAAAPAAPAAPPAPTKPELIETSKINSGDTAWMLASMALVLMMTVPGLALFYGGMVR
                     KKNVLATVMQSFAITCLVTVLWVIIGYSLAFIPGSPFIGSLDRLFLSGMSFMKEANKV
                     MVSHLAPTIPESVYMAFQMTFAIITPALIAGGFADRMKFSAMLWFMGLWSVLIYSPIA
                     HMMWESTSGYFATMGVLDFAGGTVVHINAGIAALMCALMLGKRVGYGKEAMPPHNLTL
                     TITGAALLWVGWFGFNAGSAVAADGRAGMAMTVTQLATATAALSWMFAEWIAKGKPSV
                     LGIASGAVAGLVAITPASGFVGVTGAFVIGIAAGVLCFIAATSVKKMFGYDDSLDAFG
                     VHCIGGITGALLTGIFNVKEISGVDANFMVQVYAVLATLLISGIGSLIILKVIDMTIG
                     LRVTEEEEREGLDVSLHGESVV"
     gene            complement(3043..>3121)
                     /locus_tag="AC16CX_07900"
     CDS             complement(3043..>3121)
                     /locus_tag="AC16CX_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3012470.1"
                     /translation="DGKVFVFDLEQAIRIRTGETGADAL"
BASE COUNT          609 a          949 c          995 g          568 t
ORIGIN      
        1 aggtcaggac ccggatcagc atcggcaggt tgacgccggc cacgccttca accttgccgg
       61 gcacgagcaa cttggcggcg atgttggaag gcgagccgcc gtacatgtcc gagaggatca
      121 gcacgccgtc gccctcatcg agctccttca ccagtttgcg cgcctgcggc agcagctgga
      181 acggatcgtc ctgggcagtg atgccgatct gcttgaggcg cacgggacgc ttgttgagca
      241 cgtggctggc gctatggatc aggctctcgc cgagcgtgcc gtgcgtaatg atcaaaatcc
      301 caatcataaa aacaggttac cttgggttac gaaatcgaaa tattttacgc ttttgcgtcg
      361 cacaacgcac aaggggtggg cttgacctgc ctcaagaatg aggcggcagt taaaccgctt
      421 tcgcgtgttc gcgcccggcg caggctgctt cgagcgcgtc catgaacatg cccgcgacgt
      481 tgaatccggt ctgctcggtg atctcctgga agcaggtggg gctggtgacg ttgacttcgg
      541 tcaggtagtc gccgatcacg tcgaggccga ccagcaggag gccttccttg tcgagcacgg
      601 gtcccagcgc ttccgcgatc tcgcggtcgc gtggtgtgag ttcgcgggca acaccggtgg
      661 cgcctaccgc gaggttgccg cgcgtttcac cgggcttcgg gatgcgcgcg aggcaatagg
      721 ggaccggctt gccggcgatc aacagcacgc gcttgtcgcc gttgcggatc tccgggatat
      781 agcgctgtgc catgaccgtg cgccggccgt agtgggtcac ggtctcgatg atgacgttga
      841 cgttgggatc gtcgtgccgc acgcggaata ccgacgcgcc gcccattccc tcgagcggct
      901 tcaagatcac gtcgcggtgc tcggcgagga attcgcggat gagcgattcg cgccgcgtga
      961 cgacggcggg cggcgtaaac cggggaaagc gcgcgatggc catcttctcg ttccagtcgc
     1021 ggatcgcccg cggccggttg aacacccgcg cgccctgcgt ctccgccagc tcgaggagat
     1081 aggtgctgta cacgtactcc atgtcgaacg gcgggtcctt gcgcatcagc accgcgtcga
     1141 agtccttgag tggcgtcgaa gcgggctcct cgacgcggta ccagcacggc ttctcgccag
     1201 tgaggtgcag ccttgccgcg tggccgtgaa caataccgcc gcgccacgca agctggtcct
     1261 gctccagggc gtacaaattg tgcccgcgcg cggcggcctc gcgcatgatg gcgaaggtgg
     1321 tgtccttgta gatcttgaag gaatcgagcg ggtcggcgac gaaggccagc ttcatggaat
     1381 ggtgagcctt gagcggtaag cagtgagcag tatagcgaaa gcggaaccaa gctggttttc
     1441 caaacacgac tcacgatccg cgagtcgcga ttcacggttc atcagcacaa aaaacgggcg
     1501 cccgcaggcg cccgtaagcc acatacttcc tgataaagcg ttacatatga ccgaaatcgt
     1561 ctcgcaacgg agcgttagac cacgctctcc ccgtgcagcg acacgtccag cccttcgcgt
     1621 tcttcttctt cggtgacgcg caggccgatc gtcatgtcga tcaccttgag gatgatgagg
     1681 ctgccaatgc cgctgatcag caaggtcgcc agcacggcat agacctggac catgaagttg
     1741 gcgtcaacgc cgctgatttc tttcacgttg aagatgccgg tcaggagcgc acccgtgatc
     1801 ccgccgatac agtgcacgcc gaaggcatcc agcgaatcgt cgtagccaaa catctttttc
     1861 accgatgtcg cggcaatgaa gcagagcacg cccgcggcaa taccgatgac aaaagcgccg
     1921 gtgacgccga cgaagccgga ggccggagta atagctacca agcctgcgac ggcgcccgaa
     1981 gcgatgccga gcactgaggg tttgcccttg gcaatccatt cggcgaacat ccaggacagg
     2041 gcggctgtgg ccgtggcgag ctgcgtgacg gtcatcgcca tgccggcgcg tccgtcggcg
     2101 gccactgcgg agcccgcgtt aaagccgaac cagcccaccc acagcagcgc cgcgccggtt
     2161 atggtgagcg tcaggttatg cggcggcatt gcttccttgc cgtagccgac gcgtttgccc
     2221 agcatcagtg cgcacatcag tgcggcgatc ccggcattga tgtgcactac ggtcccgccg
     2281 gcaaaatcga ggacgcccat ggtggcgaag taaccgctcg tggattccca catcatgtgc
     2341 gcgatcggcg agtagatcag caccgaccaa aggcccatga accagagcat cgccgagaat
     2401 ttcatgcgat cggcgaagcc gccggcaatc aaagcgggcg tgatgatcgc gaaggtcatc
     2461 tgaaatgcca tatacacgga ttccgggatc gtgggtgcaa ggtggctgac catgaccttg
     2521 ttcgcttcct tcatgaagga cattccggat aggaataacc gatccaggct accgatgaag
     2581 ggggaccccg gtatgaacgc caggctgtag ccgatgatga cccaaagcac cgtcaccaga
     2641 caggtgatgg caaagctctg catcaccgtg gcgagcacgt tctttttgcg caccatgccg
     2701 ccgtaaaaca aagcgagacc cgggacagtc atcatcagca ccagggccat ggacgcgagc
     2761 atccacgcag tatccccgga gttgatcttt gaggtctcaa tgagctcggg tttggtcggc
     2821 gctggaggag ccgcaggagc agcgggcgcg gcagcagcgg gcgtggtcgc cggtgccgca
     2881 gcgggggctg caggcgcggc agatggcgtc gcggcggggg cgggtgcagc cgccggcttg
     2941 tcctgcgcca acgcgaccac cggcaccgtg ttcagcccta ccacgctgaa catggccagc
     3001 actgcgatca gttttctgat gactcttttc atcggattct ccctatagcg catcggcccc
     3061 ggtctcgccg gtgcggatgc ggatggcctg ctctagatcg aagacgaaca ccttgccgtc
     3121 g
//
LOCUS       DBNGWZ010000267         2485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2799303, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000267 DBNGWZ010000000
VERSION     DBNGWZ010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2799303"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC16CX_07905"
     CDS             <1..786
                     /locus_tag="AC16CX_07905"
                     /inference="COORDINATES: protein motif:HMM:NF023472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3530 family protein"
                     /protein_id="HIR3012471.1"
                     /translation="FDSQITMNLSFASLASFALGGFTAFLLAPALALAQADYAREKRW
                     ADEITPAILVGDPVYLTLKSGRKFLAIHAPSAKATAGVIVVHGLGVHPDWGLINPLRS
                     QLAEQGYSTFSVQMPVLAAEASGDQYPPLFSEAAERLAAAVAFLRGKGNKKVAIVSHS
                     MGSRMTNYFLNHAGAARIDAWVTIGLSGEFTEPATFKAPVLDLYGENDLPAVLENAGK
                     RAAAIRGIRGSGQVQVTGADHFFAGMEQELVLRVRQFLDARLR"
     gene            1127..1591
                     /locus_tag="AC16CX_07910"
     CDS             1127..1591
                     /locus_tag="AC16CX_07910"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3012472.1"
                     /translation="MKLELRTNKDFWAAVMYISTGAVGWWIALDYPFGSALRMGPGYF
                     PIVLSAIMIAMGIYVLALGLRKDHEKIQGNWSIRALIVLPISMAVFGWLMEESGFIAA
                     MVALVIVSAAAGREFKWGEVLLLTLGLTVACAAGFIYGLGLPYPLIKGMWGY"
     gene            1593..>2485
                     /locus_tag="AC16CX_07915"
     CDS             1593..>2485
                     /locus_tag="AC16CX_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3012473.1"
                     /translation="MELFNNLVFGFSVALTLQNLWFCFVGVFLGTLIGVLPGIGPLAT
                     IAMLLPITFNVPPVAALIMLAGIYYGAQYGGSTTAILVNLPGETSAVVTCIDGYQMAR
                     QGRAGPALAIAAIGSFFAGTVCTLIIALFGPPLAEMALKFGAPEYFSLMLMGLVMAAV
                     LAHGDMIKSLAMVAMGLLLGIVGTDVNSGVLRFTFDIPELSDGIGFVIVAMGMFGATE
                     IILNLEHTGKREIFTSKVKNLWPTREDFRNAWPSVLRGTALGSALGILPGGGALLASF
                     GAYTVEKKLSNHRRSSARARS"
BASE COUNT          407 a          773 c          799 g          506 t
ORIGIN      
        1 ttcgattcac aaatcaccat gaatctttcc tttgcgtcct tggcgtcctt tgcgttggga
       61 ggtttcactg cttttctgct cgcgcccgcg ctggcgctcg cgcaagccga ctacgcgcgc
      121 gagaagcgct gggcggacga gattacgccc gcgatcctgg tgggtgaccc ggtttatctc
      181 acgctcaagt cgggacgcaa attcctcgcg atccatgcgc caagcgccaa ggccaccgca
      241 ggtgtaatcg tcgtgcacgg cctcggcgtg caccccgact ggggcttgat caatccgctg
      301 cgcagccagc tcgccgagca gggatattcc acgttctcgg tgcagatgcc ggtgctcgct
      361 gcggaagcca gtggcgacca gtatcctccg ctcttctccg aggcggcgga gcgccttgcg
      421 gctgcagtcg ccttcctgcg cgggaaggga aacaagaaag tcgccatcgt ctcgcacagc
      481 atgggctcgc gcatgaccaa ttattttctc aatcacgccg gcgctgcgcg catcgatgcg
      541 tgggtgacga tcggcctgtc cggtgaattc accgagccgg cgacattcaa ggcgccggtg
      601 ctcgatcttt acggtgagaa tgatttaccg gcggtactgg agaacgcagg caagcgcgcg
      661 gcggcgatcc ggggaatacg gggttcaggt caagtgcagg tcaccggcgc cgaccatttc
      721 ttcgccggga tggagcaaga gctggtgctc cgggtcaggc aatttctgga tgcgcggctc
      781 aggtagtaag ccccgcgtaa gctaggcatt tgccgggctt aacgcaccgc cgcagtgcgt
      841 caccaacccc ctttcccatg cttaggcacc ctcgccaatc gttaaaaagt cggctattat
      901 cctctccgtt tacatggtgg actgtcgccg cacgggcggc gcagtcccag ccagaaggaa
      961 gcggcctgcc cgaggcgtct tggccccggc gccggaggaa gggaaggccg aaaatccaaa
     1021 gtggagcggg gctctaatca cttccagaag agggggagcc gtccgtgtcg aagccggcct
     1081 gtcatccgca cgcatccatg cgtgtcttaa cctgtggagt tcctgaatga aactcgagtt
     1141 gcgcacgaac aaggatttct gggccgccgt gatgtatatc tcgaccggcg ccgtgggctg
     1201 gtggattgcc ctcgattatc cgttcgggtc ggcattgcgc atggggcccg gctattttcc
     1261 catcgtgttg agcgccatta tgatcgcgat gggcatctac gtcctggctt tgggacttcg
     1321 caaggatcac gagaagatcc agggcaactg gtcgatccgc gcgctcatcg tgctgccgat
     1381 ttcgatggcc gtgttcggct ggctgatgga agaatccggc ttcatagccg cgatggtggc
     1441 gctggtcatc gtgtcggcag ccgccggccg cgaattcaag tggggggaag tcttgttgct
     1501 caccctcggc ttgacggtgg cgtgcgccgc cgggttcatc tatggcctgg gcctgccgta
     1561 tccattgatc aagggcatgt ggggctactg atatggaact attcaacaat ctcgtcttcg
     1621 gcttcagcgt cgcgctgacg ttgcagaacc tgtggttctg cttcgtcggc gtgttcctcg
     1681 gcacgctgat cggcgtgctg ccgggcatcg gcccgctcgc gacgatcgcg atgctgctgc
     1741 cgattacctt caacgtgccg ccggtcgcgg cgctcatcat gctggcgggg atttattacg
     1801 gcgcgcagta cggcggctcc accaccgcca tcctggtgaa cctcccgggc gagacctccg
     1861 cggtggtgac ctgcatagac ggctaccaga tggcgcgcca ggggcgcgcc ggcccggcgc
     1921 tcgcgatcgc ggcgatcggc tcgttcttcg ccggcacggt gtgcacgctg atcatcgcgc
     1981 tgttcggccc gccgctcgcc gagatggcgc tcaagttcgg cgcgcccgag tacttctcgc
     2041 tgatgttgat gggcctcgtc atggcggccg tgctggcgca cggcgacatg atcaagtcgc
     2101 tggcaatggt ggcgatgggg ctgttgctcg gcatcgtcgg caccgacgtc aattccggcg
     2161 tgctgcgctt caccttcgac atccccgagc tctccgacgg catcggcttc gtcatcgtcg
     2221 ccatggggat gttcggcgcg accgagatca tcctcaacct cgagcacacc gggaagcgcg
     2281 agatcttcac cagcaaggtg aagaacctct ggccgacccg ggaggacttc aggaacgcct
     2341 ggccgtcggt cctgcgcggc acggcgctcg gctcggcgct cggcatcctg cccggcggcg
     2401 gagcgctgct cgcctccttc ggcgcctata cggtggagaa gaagctctcc aatcatcgga
     2461 gaagttcggc aagggcgcga tcgag
//
LOCUS       DBNGWZ010000268        16448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_155357, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000268 DBNGWZ010000000
VERSION     DBNGWZ010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_155357"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC16CX_07920"
     CDS             <1..624
                     /locus_tag="AC16CX_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR3012474.1"
                     /translation="MFVLQHRLNTRQRNSVLKLKVATNCTDWDTRMRIIQACSEEVIA
                     DHAHGGGRPHWQILEDLGVRIGLKRAVIQQAQPLPSTQLAWAAWEGLMANRHWLEGIV
                     ANTCAERVNVPGYGKGLIRKVGWFGLERHRWGELFKLGDEDLEFFGLHEEADIEHSNA
                     GWQAVERFARKLHMEDAVVAACEKNLYVWEHYLNGIADAGDQLRRSQ"
     gene            715..2034
                     /locus_tag="AC16CX_07925"
     CDS             715..2034
                     /locus_tag="AC16CX_07925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3012475.1"
                     /translation="MSRFFDVVIIGAGAAGLAAAAALAPSGRRVLLLEARDRIGGRIW
                     SRHEPGLPVPVELGAEFIHGRPAATFSLMRRAGITAVDAPIVRLAARSGELRPRGNDP
                     FAQVRRVLRRHAGALRKKDVSFEAFLARGLHGLSEEACIFARMRVQGYDAADPAQASA
                     RAIAEEWSGEGAADAGHFRPAGGYGALMTSLAEAFHGSGVELRLETIVREVRWRRGSV
                     EVVGTFRGGVFRAAAPRAIITLPLGVLKQPPRAPGAVRFTPALKEKQAALRGLASGAA
                     IKIPLLFRTAFWEELEEGRYRDVSFFHSPEAAFPTFWSALPERAPLLIAWAGGPNATR
                     LAGASTRDITREAVASLESIFGKRTGIEERLDAVWVHDWQQDPFACGAYSYVAVGGHG
                     ARRALAAPVRDTLYFAGEATDFEGEHGTVAGALQSGARAARMLMRDW"
     gene            2245..2829
                     /locus_tag="AC16CX_07930"
     CDS             2245..2829
                     /locus_tag="AC16CX_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012476.1"
                     /translation="MPYSLTISHVGIFVNDMPRMVDFYTRFLGYAVSDRGIRSGGGEI
                     VFMTRDPCEHHQLVLASGRPADLRFNLINQISFRVDSLATLRELHDGLENEAVKEITT
                     LTHGNALSVYFRDPEYNRIELLIDTPWYVPQPHHLMVDFSLPDGELWAAIEKHVRSMP
                     GFKPQPEWAAEIERKIAHATANRQTARGRATSHA"
     gene            2849..3895
                     /locus_tag="AC16CX_07935"
     CDS             2849..3895
                     /locus_tag="AC16CX_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012477.1"
                     /translation="MKIKAEGRRQKAEGRRQQARRAGRFHFILYSSAFILGCAVAVGV
                     ATAQPYPAKPVRIMVGYVPGGGVDTTARLVAAALSDLWGTTVFVENRPGAAGGIATEV
                     TAKAPPDGYTLMLCQIASHAITPARSRKLPYDHIRDFAFPSMIGAVPNVFMVHPSMPT
                     KTLRDYIALARAHPGEVNFGSSGIGASPHMSIELLKTMTRINIVHVPYKGAAAALSDV
                     MSGHIESSVGNLAGGPLAAIKAGRVRALGVTSAKRNKQVPDVPTFSEAGVPGFEVTAW
                     YGICTQSKVPQPIVARLHADLVKVLSGPELLKRLEDQGIDVAPSTPEQFVAHVRAETE
                     KWTKVVRAAGLEER"
     gene            3900..5216
                     /locus_tag="AC16CX_07940"
     CDS             3900..5216
                     /locus_tag="AC16CX_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3012478.1"
                     /translation="MEKGKKQELAIEPFEMDLSLQVGKYEVQKLLGKGATGTVYHAKD
                     TFSGKEVALKTIEPEVFRDPEFGTVYRQQFLNEASLAGKLKHPHIVGILDAVVQEHSG
                     YIAMELVTEGDLSRYAKPGKLLPVADVLQIGFKCCGALDYAFKEGIVHRDIKPANLMI
                     ARGTDVRISDFGAAYLRKSQVVQTASMGSPFYMSPEQIRGENLTFHSDMYSLGVVLYE
                     LLTGHRPFTAKNMDALIKKILEQAPAPPTKLRADLPKEIDAIVLRALAKTPEQRYPTW
                     TDFALKLSRLGDQVLPPGVIPDSEKYIALKKVPMLSNLSDAELWELALAGKWREVPAK
                     KTIVKENEKGLSFFLVGRGQVKVTRQGRLLNTLDERECFGEMAYIRGGVVPRHATVES
                     VTELLLAEFDPETLAKMSISAQYNLMRALVRNLVDRLEEANIRLSR"
     gene            5400..6389
                     /locus_tag="AC16CX_07945"
     CDS             5400..6389
                     /locus_tag="AC16CX_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012479.1"
                     /translation="MKKGSTGRKHTAARALALSAVLLAAQPLLAQPYPARPVRLIVPY
                     AAGGPVDTVGRLVAQNLTGRWGQQVIVDNRAGSGGALGTQVVVKSPPDGYTLLVGNSG
                     PITAYPHLRKNLLYDHERDLAPASFMLKSCMVLVAHPSLPAKSVQDLVRLAKKDPAGL
                     SYSSSGVGGIQHLGMVLLESLAGIRLVHVPYKGAAPALMDLIAGQVHVQFNNVVGSLP
                     HVQAGKLRALGVSTATPSPVLPGVPPVAGAYPGFDVASWMGIYAPAGTPKPVIDQLTR
                     DVAWALNVPEAKQRLTELGAEIVAGGPAELGAYARSESRLFGKLISSAGIPKE"
     gene            6396..7361
                     /locus_tag="AC16CX_07950"
     CDS             6396..7361
                     /locus_tag="AC16CX_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012480.1"
                     /translation="MAASKIGSAAAMALVLVCATASAQPYPTRPIRIIVAAAPGGGTD
                     FVSRLMGQKLAEALGQQVFVENRPGAGSTIGYEYGVKVAPDGYTLTMITGSYSINPSL
                     YPIRFNPLNDFTPVIWVARGPYVIVVHPSLPVRNTRELIALAKARPGQIIYGSSGQGA
                     IVHLTTELFLYMSGVKMTHVPYKGGGPAMTDLLAGHIQLVFATVPVGLPQVKAGRLRA
                     LAVTTSERVPAEPGIPTVAESGVPGYEVTNWHGLIGPKGLSRPVVERINSEMTKIIRQ
                     KEMEERLQADGLSPVGGTPEQLYEQIRKEIEQWRQVVQRAGIRIN"
     gene            7441..8373
                     /locus_tag="AC16CX_07955"
     CDS             7441..8373
                     /locus_tag="AC16CX_07955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012481.1"
                     /translation="MDALVLAVPAWAQGYPSRPIRIIVPFGPGGPSDLLARMAGQRLT
                     EAWGQQVIIDNRPGANGVVGSELAAKSAPDGYTLVMATNGTHGINASLFPKLPYDTVR
                     DFAPITRLGLAPYLLVAHPSLPVRSVKDLIQLARARPGEITWSSGGSPTQLAAELFKK
                     TARINAVVVPYKGNAPAVSAVISGEVSLVFGGIAQSAPQVKAGRLRALGVASLQRSAV
                     IPEVPTITESGLPGFEASAWYGLLAPGATPRAIVERLNGEVVRILRLPEVQQRLRGEA
                     FETPADTPERFAAVIRAELAKWAPIVKEAGVRPD"
     gene            8523..8930
                     /locus_tag="AC16CX_07960"
     CDS             8523..8930
                     /locus_tag="AC16CX_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3012482.1"
                     /translation="MERKKLLTAPPDTTVSEAAKRMARRKVGAVMVVEHESLVGIFTE
                     RDVVFRVIAQGRDAQTTRLAEVMTTSPQTVDPGKSFGYALLMMHEKGFRHVPVIENGK
                     VIGIVSARNALDPDLEEFVAESQRRMQILRERA"
     gene            9179..9880
                     /locus_tag="AC16CX_07965"
     CDS             9179..9880
                     /locus_tag="AC16CX_07965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3012483.1"
                     /translation="MRLIIRVLASLLIMSTSFAPAAQAADTDTAYVVSYFETTPAVKD
                     KAAGLLRQLARQSRKEAGNLRFEALQRVGQPDQFVVLEAWKDKDAQAAHAAADRTKQF
                     RDKLQSLLRGPYDERPHTALEVGDVKSAPAGAVKKTAIYAVTHVDIVPTQKETGIGLV
                     KQLAEDGRRDKGNVRFEALTQSSRTNHMTVVEIWPDRGALEAHGTTAHKKQFREKVMP
                     MSGSLYDERLYRALD"
     gene            complement(9960..11117)
                     /locus_tag="AC16CX_07970"
     CDS             complement(9960..11117)
                     /locus_tag="AC16CX_07970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012484.1"
                     /translation="MASGLTPIRRVVTGSDARGRSKVVWDGPAPNTHEASMGSGRGHT
                     DLWVWTETSVPLSRDSDDGNLKYVFSGPPEGGHLRVVQIRPRPADYDAAKDKEVVPVH
                     PARPRPGSRGTWDRGGNNSYSSAMHKTETVDYGIVLAGERGLILDDRELTMKPGDVVV
                     QVGAWHQWTMPKGAIMAFDMIAARFVDGPAGVAQGNDAPMRATPKLPDGVKPTRRIVT
                     IDREPGRSSLVSDGPAPDVRTDPARPGYACARLWVTDGAPAKIVFETLRLPHVLEPPA
                     HGTVFRVVTFPPDDSWKGKVGAAEVRAFFRDMGSPGASTYSPLAPHPYMQKTRTLDFC
                     IVLEGEIVLVLDTQEVRLKAGEIAIQRGTNHAWSNRSGRPAVVAIASHDGR"
     gene            11813..13060
                     /locus_tag="AC16CX_07975"
     CDS             11813..13060
                     /locus_tag="AC16CX_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HIR3012485.1"
                     /translation="MVVEAAIIAVFFVVYLGMILGGLPFLQLDRTGIALLGAIALVSI
                     GALSLEEAWGAIHAPTLILLFAFMVISAQLRLGGFYTWVTWRLGKLPLTPPGLLAALV
                     FVIAALSAVFSNDIICLAVAPVLMEVCHARRLNPVPFLLALACAANVGSAATLIGNPQ
                     NMLIGETLKLPFAGYMREAGLPVILGLVVTWALIAWQARGRWEAMPAGSAPTAGHPAG
                     EAPPPFDRWQSVKGLSVAAAVFVLFLYAPLPREVVALVAAGLLMTSRKLHSYKMLGLV
                     DWQLLVLFIGLFVVNHGLAKTGMPQHVIEDSAATGVDLAHPGPLFAAAFVLSNVVSNV
                     PAVMLLLPAATHEIAGPVLALSSTLAGNLLVVGSIANIIVIGAAQRHHVLIGWTEHAR
                     VGVPVTLATIAIAAAWLWFRLQG"
     gene            13434..15128
                     /locus_tag="AC16CX_07980"
     CDS             13434..15128
                     /locus_tag="AC16CX_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3012486.1"
                     /translation="MRASNSKGAVKALAVAGTRVVALGWDMSAADIKSRKVLGFAIER
                     TRKRDGEVIWMRGTKTFESVLKDPGQGVTVSTRDHPFQSFQWSDYSVEPDQTYTYRIV
                     ARTGTPGGLKDGPSAKLEVTTESEEIGKHMVFFNRGSVASQEYARRFKNKSPEVVGQA
                     AWDWLSRGLIEALEKFIGQAGEGDEIHGAIFEFKHKRVFDALKAAKKRKAKIFIIYDG
                     QTQGSSNRKELNANGVFNWLKEDTQIRARKHSGRYAHNKFFVLSRNGTAKQVWTGSTN
                     LSVNGIFGHSNNAHVVRDEKVARAFRAYWERLWDDPTKKPLATEDAKKTPAPPPQWLD
                     ETVPMFSPQPDVSPLDWYATLAGKPKKPLFTTFAFGMNEKFVPIYGRRDGVLRFALME
                     KKGNGRQIKEQSRVIDRIRRLPNTVVAVGDAVDKDNTFDRWLVERVKIVNEAHVLFVH
                     TKYMLIDPLGKDPIVIVGSANFSKASCDTNDENMLIIRGNATVADIYLGEFMRLHTHY
                     AYRESLGFRRTSEKDYERRRKYLVESIDWIKGEAQGQGYFDKGGARALRRAYFSGG"
     gene            15433..>16448
                     /locus_tag="AC16CX_07985"
     CDS             15433..>16448
                     /locus_tag="AC16CX_07985"
                     /inference="COORDINATES: protein motif:HMM:NF021508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HIR3012487.1"
                     /translation="MKRLVICCDGTWNTPDQKDRGVMRPTNVVKLARWVMPQAPDGTV
                     QRVHYDRGVGTGDIIDRAFGGMFGIGLAHNVREAYEFLSQNYDDGDQVFLFGFSRGAY
                     TVRRTVGMVRKCQLLPKDLGDETRKAAIEEGYEIYTRREGEKQGGPDSEAAQAFRRKY
                     GCRPVTIRCLGVWDTVGAYGIGGVLGQLTSSFTKARFHDRRLSSIVEHACQAVAIDET
                     RRLFQPNLFEQGPAGLTKGQVLEQSWFAGVHSNVGGGYEDTGLSDVTLHWMAMRAEQC
                     GLALDPKWRDRIDPDEFGELRESRTGLYLLMGKAVRPIGRQKNGFEKAHNATMDRMAR
                     DPAG"
BASE COUNT         2949 a         5196 c         5509 g         2794 t
ORIGIN      
        1 atgttcgtgc tgcagcaccg cctcaacacg cgccagcgca actcggtgct caagctcaag
       61 gtcgcgacca actgcaccga ctgggacacg cggatgcgca tcatccaggc gtgcagcgag
      121 gaggtgatcg ccgatcacgc gcacggcggc ggccggccgc actggcagat cctggaggac
      181 ctcggcgtgc gcatcgggct caagcgcgcc gtgatccagc aggcgcagcc gctgccctcg
      241 acgcagcttg cgtgggcggc gtgggaaggg ctgatggcca acaggcattg gctcgaaggc
      301 atcgtcgcca atacctgcgc cgagcgcgtc aacgtgcccg gctacggcaa ggggctcatc
      361 cggaaagtgg gctggttcgg actcgagcgg caccgctggg gggagctgtt caagctcggt
      421 gacgaggatc tcgagttctt cgggctgcac gaggaagcgg acatcgagca cagcaacgca
      481 gggtggcagg cggtggagcg gttcgcgagg aagctgcaca tggaggacgc ggtggtcgcc
      541 gcgtgcgaga agaacctcta cgtgtgggag cactacctga acggtattgc cgacgcgggc
      601 gatcagctcc gcagatcgca gtgataagtg ataagtgata agtgatagga gcacggttcc
      661 gcgcggagtg cggatttcat atcacttatc acctgtcact catcacgctt ttccgtgtca
      721 cgctttttcg acgtggtgat catcggcgcg ggggccgcag ggctggcggc cgcggcggcg
      781 ctcgcgccgt ctgggcggcg ggtccttctg ctcgaggcgc gcgaccgtat cggcgggcgc
      841 atctggtcgc gtcacgagcc cgggttgccg gtccccgtcg agctgggcgc cgagttcatc
      901 cacgggcgtc ccgcggcgac gttctccctg atgcgcaggg ccggaatcac ggcggtcgat
      961 gcgcccatcg tccgcctcgc ggcccggagc ggggaactcc ggccaagggg gaacgacccc
     1021 ttcgcgcaag tgcggcgggt gctgcggcgg cacgccggcg cgctcaggaa gaaggatgtc
     1081 tccttcgagg cgtttctcgc gcgcgggctg catggtttgt ccgaagaagc ctgcatcttc
     1141 gcccggatgc gggtgcaggg ttacgacgcg gctgatccgg cgcaggccag cgcccgggcg
     1201 atagccgagg agtggagcgg cgagggcgcg gctgacgccg gccacttccg gccggcgggc
     1261 ggctacggcg cgctgatgac gtcgctcgcc gaggcatttc acggcagtgg agtcgagctg
     1321 cggctcgaga ccatcgtgcg cgaagtgcga tggaggcggg gatcggtcga ggtcgtagga
     1381 accttccgcg gcggggtttt ccgcgcggcc gcgccgcgcg cgatcatcac gctcccgctc
     1441 ggggtgttga agcagccgcc gcgcgcgccg ggcgcggtcc gtttcacgcc ggcgctcaag
     1501 gaaaagcagg cggcgctccg agggctcgcc tccggagccg ccatcaagat cccactcctg
     1561 ttccgcaccg cgttctggga agagctggaa gaagggcgct accgggacgt ttcgttcttt
     1621 cactcgcctg aggcggcgtt tccgactttc tggagcgcgc tgccggagcg cgcgccgctg
     1681 ctgatcgcgt gggcgggcgg cccgaacgcg acgcgccttg ccggcgcctc gacgcgggac
     1741 atcacccgtg aagcggtggc gagcctggaa tccatcttcg gcaagcggac cggcatcgag
     1801 gagcggctcg acgccgtctg ggtgcacgac tggcagcagg acccgttcgc gtgcggcgcc
     1861 tacagctacg tcgccgtcgg cggccacggc gcgcgcagag cgcttgccgc gcccgtgcgg
     1921 gacacgctct acttcgccgg cgaagccacg gacttcgaag gcgaacatgg gaccgtcgcg
     1981 ggcgcgctgc aaagcggcgc gcgggctgcg cgcatgttaa tgcgtgactg gtgaggcaca
     2041 gagtcggcag ttggcagccg tcaataggct acaaccgcgt gataagtgat aagtgataag
     2101 ttttccgcgg agcggaagtc gttaacttcc tatcacctat catgcttttt tcctatcctg
     2161 catctcgcat cccgcggtgt tgctccctcc gttcatgatt tggcagtaga ctatgccctt
     2221 ttccgccaat cgaggagaag ccgcatgccg tattcgctca cgatcagcca cgtcggcatc
     2281 ttcgtcaacg acatgccgcg catggtggac ttctacacgc gtttcctggg ctacgccgtc
     2341 agcgaccggg gcatacgttc gggcggcggc gagatcgtgt tcatgacccg cgacccgtgc
     2401 gagcaccatc agctcgtcct ggcgtccggg cggcccgccg atctccggtt caacctgatc
     2461 aaccagatct cgttccgtgt ggacagcctg gccacgctgc gggagctgca cgacggcctc
     2521 gagaacgaag cggtgaagga gatcaccacg ctcacgcacg gcaacgcgtt gtcggtctat
     2581 ttccgcgatc ccgagtacaa ccggatcgag ctgctgatcg acacgccgtg gtatgtgccg
     2641 cagccccatc acctgatggt ggacttctcg ctgccggacg gcgagctgtg ggcggccatc
     2701 gagaagcacg tccgctccat gcccggtttc aagccgcagc cggaatgggc ggcggaaatc
     2761 gagcgcaaga tcgcccacgc caccgcgaac cgccagaccg cccgcgggcg ggcgacgagt
     2821 cacgcctgac gggcgcaagg gatgggagat gaaaattaag gcagaaggca gaaggcagaa
     2881 ggcggaagga agacggcagc aggcaagacg agccggaagg tttcacttca ttctttattc
     2941 ttctgccttc atcctcggct gcgcggtcgc ggtcggcgtc gcgaccgcgc agccataccc
     3001 ggccaagccg gtgcgcatca tggtcggtta cgtgccgggc ggtggcgtgg acacgaccgc
     3061 gcgcctcgtc gccgcggcgc tctccgacct gtggggcacc acggtattcg tggaaaaccg
     3121 gcctggcgcg gccgggggca tcgcgaccga ggtcacggcg aaggcgcccc ccgacggcta
     3181 cacgctgatg ctgtgccaga tcgcctccca tgcgatcacg ccggcgcggt cgaggaagct
     3241 gccctacgat cacatccgcg acttcgcgtt tccttcgatg atcggcgcgg tgcccaacgt
     3301 cttcatggtg catccctcga tgccgacgaa gacgctgcgc gactacatcg ctctcgccag
     3361 ggcacacccg ggcgaggtca acttcggctc ttccggcatc ggcgcctcgc cgcacatgtc
     3421 catcgagctg ctgaaaacga tgacccgcat caacatcgtg cacgtgccgt acaagggcgc
     3481 agcggcggcg ctctccgacg tgatgagcgg gcacatcgag tcctccgtcg gcaacctggc
     3541 tggggggccg cttgccgcga tcaaggccgg cagggtgagg gcgctcggcg tcacctcggc
     3601 gaagcgcaac aagcaggtgc ccgatgtgcc gacgttctcc gaagccggtg tgccgggatt
     3661 cgaggtcacg gcgtggtacg gcatctgcac gcagtcgaag gtgccgcaac ccatcgtcgc
     3721 caggctccac gccgacctgg tgaaggtcct gagcggtccg gagctgctga agcgcctcga
     3781 agaccagggc atcgacgtcg ccccgtccac gcccgagcag ttcgtcgccc acgtcagggc
     3841 cgagacggag aagtggacga aagtggtgcg cgcggcggga ctggaagaac ggtgagcgca
     3901 tggagaaggg caaaaaacaa gagctggcga tcgaaccgtt cgaaatggac ctgagcctcc
     3961 aggtcgggaa atacgaggtc cagaagctcc tcggcaaggg cgcgaccggg acggtttacc
     4021 acgccaagga cacgttttcc ggcaaggaag tcgcgctcaa gacgatcgag cccgaagtct
     4081 tccgcgaccc cgagttcggg accgtctacc gccagcagtt cctgaacgag gcttccctcg
     4141 ccggcaagtt gaagcacccg cacatcgtcg gcattctcga cgcggtggtg caggagcatt
     4201 ccggctacat cgcgatggag ctcgtcaccg agggcgacct ctcgcggtac gccaagcccg
     4261 gcaagctgct gccggtcgcc gacgtgctcc agatcggctt caagtgctgc ggcgcgctcg
     4321 actacgcctt caaggagggc atcgtccacc gcgacatcaa gcccgcgaac ctgatgatcg
     4381 cgcgcggcac cgacgtgagg atctcggatt tcggcgcggc ctacctgcgc aagagccagg
     4441 tggtgcagac ggcgtccatg ggctcgccct tctacatgtc gcccgagcag atccggggcg
     4501 agaacctcac cttccacagc gacatgtact cgctcggcgt cgtgctctac gagctgctga
     4561 ccggtcatcg gccgttcacg gcaaaaaaca tggacgcgct gatcaagaag atcctggagc
     4621 aggcgcccgc gcccccgacc aagctgcgcg ccgacttgcc gaaggagatc gacgccatcg
     4681 tgctgcgtgc gctcgcgaag acgcccgagc agcgctaccc gacctggacc gatttcgcgc
     4741 tcaagctgtc caggctgggc gatcaagtgc tgccgcccgg cgtgatcccg gacagcgaga
     4801 aatacatcgc gctcaagaag gtgccgatgc tctcgaacct gtccgacgcg gagctgtggg
     4861 agctcgccct tgcggggaag tggagggaag tcccggcgaa gaagacgatc gtgaaggaaa
     4921 acgagaaggg gctgagcttc ttcctcgtcg gcagggggca ggtcaaggtc acccgccagg
     4981 ggcgcctgct caacacgctc gacgagcgcg agtgcttcgg cgagatggcc tacattcgcg
     5041 gcggcgtcgt gcccaggcac gcgaccgtgg aatccgtgac cgagctgctg ctcgccgagt
     5101 tcgacccgga gacgctcgcc aagatgagca tcagcgcgca gtacaacctc atgcgcgcgc
     5161 tcgtgcgcaa cctcgtggac cggctggagg aggccaatat caggctctcg cggtagtgcc
     5221 cgatgaagcg tgactgacat cgcgtgactg gtaaagcgtg actgatgagc gtgactgatg
     5281 gagcgcgccg gataacttgg cagccgtcag ttttcagctg tcagcagttg gccgacggta
     5341 agcagcaact gcaaactcgg cttatcgaat cacgactcac gattcacgca tcatccatca
     5401 tgaagaaagg aagcacgggc aggaagcata ccgccgcgcg agcgcttgcg ttatccgctg
     5461 ttctgctcgc cgcccagccg ttgctcgcgc agccgtatcc cgccaggccg gtgcggctga
     5521 tcgtgcccta cgcggcgggc gggccggtgg acacggtcgg gcggctcgtc gcgcagaacc
     5581 tcaccggcag gtgggggcag caggtgatcg tggacaaccg cgcgggcagc ggcggcgcgc
     5641 tgggcacgca ggtggtcgtc aagtcgccgc ccgacggcta cacgctgctg gtggggaaca
     5701 gcggcccgat caccgcctac ccgcatctgc gcaagaatct cctctacgac cacgagcgcg
     5761 atctcgcgcc ggcttcgttc atgctgaagt cctgcatggt gctggtcgcc catccttcgc
     5821 tgcccgcgaa gtcggtgcag gacctcgtcc gcctggcgaa gaaggatccg gccgggttga
     5881 gctactcgag ctccggcgtg ggcgggatcc agcacctcgg catggtgctg ctcgagtccc
     5941 tcgcggggat acggctcgtc cacgtcccgt acaagggcgc cgcgccggcg ctgatggacc
     6001 tgatcgcggg acaggtgcac gtgcagttca acaacgtggt gggctcgctg ccgcacgtgc
     6061 aggcggggaa actccgcgcg ctcggcgtct cgacggcgac gccctcgccg gtgctgccgg
     6121 gcgtgccgcc cgtggccggg gcctatcccg gcttcgacgt cgcgagctgg atggggatct
     6181 atgcccccgc cggcacgccg aagccggtca tcgaccagct gacccgcgac gtcgcctggg
     6241 cgctcaacgt ccccgaggcg aagcagcgcc tgactgagct cggcgcggag atcgtggctg
     6301 gcgggccggc ggagctgggc gcttacgcgc gcagcgagag ccggctcttc gggaagctga
     6361 tttcctcggc cggcattccc aaggagtagc ggaaggtggc ggcgagcaaa attgggtcgg
     6421 ctgcagcgat ggcgctggtg ctggtttgcg ccaccgccag cgcgcagccg tatcccacgc
     6481 ggccgatacg catcatcgtg gccgccgcgc ccggaggcgg cacggatttc gtctcccggc
     6541 tcatgggaca gaagctcgcg gaagcgctcg ggcagcaggt cttcgtcgag aaccgtcccg
     6601 gcgcgggcag cacgatcggc tacgaatacg gcgttaaggt ggcgccggac ggctacacgc
     6661 tgaccatgat cacggggagt tattcgatca accccagcct gtacccgatc cggtttaacc
     6721 cgctcaacga tttcacgccc gtgatctggg tcgcgcgcgg accgtacgtc atcgtcgtcc
     6781 acccgtcgct gccggtccgg aatacgcgcg agctgatcgc gctggcgaaa gctcggcccg
     6841 ggcagatcat ctacggcagc agcgggcagg gcgcgatcgt gcacctcacg accgagctct
     6901 ttctgtacat gtcgggggtg aaaatgacgc acgtcccgta caagggcgga ggtccggcga
     6961 tgacggatct cctggccggt cacatacagc tcgtttttgc gactgtgccg gtgggcctgc
     7021 cgcaagtcaa ggcgggacgg ctgcgcgcgc ttgcggtgac gacttcggaa cgcgtgcccg
     7081 cggaacccgg catcccgacc gtcgccgagt ccggcgtgcc cggctacgag gtcaccaact
     7141 ggcacggtct aatcggcccg aaggggctgt cacgcccggt cgtggagcga atcaatagtg
     7201 agatgacgaa gatcatcagg cagaaggaga tggaagagcg gctccaggcc gatggcctgt
     7261 ccccggtcgg cggcacgccc gagcagctct acgagcagat ccggaaggag atcgagcaat
     7321 ggcggcaggt agtccagcgt gcgggaatca ggatcaactg actgagaatc gtgataagtg
     7381 atgggtggta tggtcggccg gtgcagcggc ttgcgcttgt tgctgcgtag tgtcgtattg
     7441 ttggacgcgc tcgttctggc agtgcctgcc tgggcgcagg gctatccctc ccgtcccatc
     7501 cgcatcatcg ttccgttcgg gccgggcggc ccctccgatc tgctggcccg catggccggc
     7561 cagaggctca ccgaggcctg gggacagcag gtgatcatcg acaaccggcc tggggcgaac
     7621 ggcgtggtgg gctcggagct ggcggctaaa agcgcgccgg acggatacac gctggtaatg
     7681 gcgaccaacg gcacgcacgg catcaacgcg agcctgttcc cgaaactgcc gtacgacacg
     7741 gtcagggact tcgcgccgat cacccggctg gggctcgcgc cgtacctgct ggtcgcccac
     7801 ccgtcgttgc cggtgcgctc ggtgaaggac ttgatccagc tcgcgcgcgc gaggccgggc
     7861 gagatcacct ggtcgtccgg cggcagcccg acgcagctcg ccgccgagct gttcaagaag
     7921 acggctcgga tcaacgcggt ggtcgtgccc tacaagggca acgcgcccgc cgtgtccgcc
     7981 gtgatcagcg gcgaagtatc gctcgtgttc ggcggcatcg cgcaatcggc cccgcaggtg
     8041 aaggccgggc ggctgcgcgc gcttggcgtg gcgagcctgc agcgctcggc cgtgatcccg
     8101 gaggtgccga cgatcacgga atccggcctt ccgggcttcg aggcctcggc gtggtacggg
     8161 ctgctcgcgc ccggcgccac gccgcgcgcc atcgtcgagc gcctcaacgg cgaggtcgtc
     8221 aggatcctgc gattgcccga ggtccagcag cggctgcgcg gcgaagcgtt cgagactccc
     8281 gccgacacgc ccgagcggtt cgccgccgtg atccgcgcgg agctcgccaa atgggcgccg
     8341 atcgtgaaag aagcgggcgt ccgccccgac tgaagaggcg atgatttttg atataggtca
     8401 aatcattgac ttcgaaaggt caataccatg ttcgatggga aggcaacgaa cagaccgggt
     8461 tgcgcgcacg attcgatgca agattggggg gtgtcatgtt cagccaacgg gttaggagcg
     8521 tcatggagcg aaagaagctg cttaccgctc ctccggatac caccgtcagc gaagccgcca
     8581 agcggatggc gcgcaggaaa gtcggcgcgg tgatggtcgt cgagcacgaa agcctggtcg
     8641 ggatttttac ggagcgcgac gtggtgttcc gcgtcatcgc gcaaggccgc gatgcgcaga
     8701 ccacgcggct cgccgaggtg atgacgacct cgccgcaaac cgtcgatccc ggaaagtcat
     8761 tcggctacgc cctgctgatg atgcatgaga aaggcttccg ccacgtgccc gtgatcgaaa
     8821 acggcaaggt cattggaatc gtatccgccc gcaacgcgct ggatcccgat ctggaggagt
     8881 tcgtggcgga atcgcagcgg cgaatgcaga tcctccgcga gcgggcttga gatcgcgtgg
     8941 ccgggattcg cccggtcgcc agccggtgac cgctcgttgc cgggagcggt ctgggtcaga
     9001 caccgcccgt caatggtgac gaggtgatgt acggcgcata atttccttcc tcaccggcag
     9061 taatccgcct tttccgtcac tgttacggcc ctgtcaccgc ccatgctttc cgatcctgcg
     9121 aggaaggcgt aggctgtagt gcgcgatcgg atcgcgctca agggaaaggg ggaatgccat
     9181 gcgactgata atccgtgtgc tggcgagcct gctgatcatg tcaaccagtt tcgcgccggc
     9241 cgcgcaggca gccgacaccg acaccgccta cgtggtgagc tacttcgaga ccacgcctgc
     9301 ggtcaaggac aaggcggcgg gtctcctgcg gcagttggca cgccaaagcc gcaaggaggc
     9361 gggcaacttg cgctttgagg cattgcagcg cgttggccag cccgaccagt tcgtcgtgct
     9421 ggaggcgtgg aaggacaagg atgcgcaggc cgcccatgcc gcggcggacc gcacgaagca
     9481 gttccgcgac aagctccagt cgctgctgcg cgggccctac gacgagcgcc cgcataccgc
     9541 gcttgaggtc ggcgacgtga agtcagcgcc cgcgggagcg gtgaagaaga ccgcaatcta
     9601 cgccgtgacg cacgtggata tcgttccaac acagaaggag accggcatcg ggctcgtcaa
     9661 gcagctcgcc gaggatggcc gcagggataa gggcaacgtg cgtttcgagg cgctcacgca
     9721 gagcagccgc accaatcaca tgacggtggt ggagatctgg cccgaccgcg gagcgctcga
     9781 agcgcacggg acgaccgcac acaagaagca gttccgcgaa aaggtcatgc cgatgagcgg
     9841 cagcctctac gacgagcggc tttatcgcgc gctcgactga acgctcacgc aatcatgcga
     9901 cggggtgacg aggtgacggg aaaaggtgac gatcgttact tcgtcactgc gtcaccggtt
     9961 taccgcccgt cgtgagacgc gatcgcgacg actgcgggcc gtcctgaccg attgctccac
    10021 gcgtggttgg tgccgcgctg gatcgcaatc tccccggcct tgagcctgac ttcctgcgtg
    10081 tcgagcacga gtacgatttc gccttccagc acgatgcaga aatcgagggt tctcgtcttc
    10141 tgcatgtagg ggtggggcgc caggggggag taagtggaag cgccgggcga gcccatgtca
    10201 cggaagaacg cccggacctc ggccgcgccc accttgcctt tccagctgtc gtcgggcgga
    10261 aaggtgacaa cccggaagac ggtgccgtgc gccggcggct cgaggacgtg gggtaggcgc
    10321 agcgtctcga acacgatctt cgccggcgcg ccgtcggtga cccacaggcg cgcgcaggcg
    10381 tagcccggcc gcgccgggtc ggtgcgcacg tcgggcgccg ggccgtcgct caccagcgag
    10441 gatctgcccg gctcgcggtc gatggtgacg attcttcgcg tgggtttcac gccgtcgggc
    10501 agtttgggag ttgcgcgcat cggcgcatcg ttcccctgcg ccacgcccgc cggtccgtcc
    10561 acgaagcgcg cggcgatcat gtcgaaggcc atgatggcgc ccttcggcat ggtccactgg
    10621 tgccacgcgc caacctggac gacgacgtcg ccgggcttca tcgtgagctc gcgatcgtcc
    10681 aggatcagcc cgcgctcgcc cgcgagcacg atgccgtagt ctacggtctc ggtcttgtgc
    10741 atggccgatg agtacgagtt gttgccgccc cggtcccatg taccgcggct gccgggccgc
    10801 ggcctcgcgg ggtggaccgg caccacctcc ttgtccttcg cggcgtcgta gtccgccggc
    10861 ctcggacgta tctgcacgac gcggagatgc ccgccctcgg gaggacccga gaagacgtac
    10921 ttcaggttcc cgtcgtcgct gtccctggaa agcgggacgg aggtctcggt ccacacccac
    10981 aagtccgtgt ggccgcggcc ggaacccatc gacgcttcat gggtgttcgg cgccgggccg
    11041 tcccatacga cctttgaccg gccgcgagcg tcgctgccgg tcaccacgcg ccggatcggt
    11101 gtcaggcccg atgccatagg ttcttgctcc cccttgggaa ggatcatgtt gccgtttgat
    11161 tatgacacga cggaaatggt gcaaggcaga aggcagaagg cagaaggcag aaggcaggag
    11221 gcaacaacgc gccgatcaac ggttttttcc ttcttccttc atccttcttc ctcagggtca
    11281 tcgacccacg aatcacgatg aagtttgatt aagatcaaaa ccaggcgcgc gtgaacggcg
    11341 taaaaaggaa tcgcctcgcg tcccatctag gtggtccggc atgccgattc cggacagtcc
    11401 gggacgtggg agcaggggcc ggcgatgttg attcccccaa ccggcccgaa caactgcctc
    11461 taggcagggg ccacgaacgc cggccgatgc tgccccggca tccggtaccc ggtatgggaa
    11521 caaacggcaa aacgtggccg acatcggcac agccgggcgc ctctgaaggc gcccggcgtt
    11581 tttttgtccc ggcggctcgt atcatgcctg tccgcccgcc cgcggcgcca gctgcggaga
    11641 gcagctacac tgaaagctgg ctcaaaaacc cattgcagcc gccgatgcac gccgatggac
    11701 gctgataata tccggaccag aggcatcgcc agcaggaaat ttcatctgcg tttatcggcg
    11761 tttatctgcg gctaatgctt cgttttgatt tttgagatag gttctaccgc acatggtagt
    11821 cgaagccgcc atcatcgcgg tcttcttcgt cgtctatctc ggcatgatcc tgggcgggct
    11881 gccgttcctg cagctcgacc gcaccggcat cgcgctgctc ggcgcgatcg cgctggtgtc
    11941 catcggggcg ctctcgctgg aggaggcgtg gggggcgatc cacgcgccga cgctgatcct
    12001 gctgttcgcc ttcatggtga tctcggcgca gctgcgcctg gggggcttct acacctgggt
    12061 cacctggcgg ctgggcaagc tgccgctgac gccgccgggg ctgctcgccg cgctggtgtt
    12121 cgtgatcgcg gcgctctccg ccgtgttcag caacgacatc atctgcctcg cggtcgcgcc
    12181 ggtgctgatg gaggtctgcc acgcgcggcg gctcaacccg gtgccgttcc tgctcgcgct
    12241 cgcgtgcgcg gcgaacgtcg gctccgcagc gacgctgatc ggcaaccccc agaacatgct
    12301 gatcggcgag acgctgaagc tgccgttcgc gggctacatg cgcgaagccg gattgccggt
    12361 catcctcggg ctcgtcgtca cctgggcgct catcgcgtgg caggcgcgcg gccggtggga
    12421 ggcgatgccg gccgggagcg cgcccacggc cgggcatccg gccggcgagg cgccgccgcc
    12481 cttcgaccgc tggcagagcg tgaagggatt gagtgtcgcg gccgccgtct tcgttctatt
    12541 cctctacgcg ccgctgccgc gcgaagtggt ggcgctggtg gcggcgggcc tgctcatgac
    12601 cagccgcaag ctccactcgt acaagatgct ggggctggtg gactggcagc tgctggtgct
    12661 cttcataggg ctgttcgtcg tcaatcacgg gcttgcaaag accgggatgc cgcagcacgt
    12721 gatcgaagac tccgccgcaa cgggcgtgga cctcgcccat cccgggccgt tgtttgcggc
    12781 cgcctttgtg ctatccaacg tcgtgtccaa cgtgccggcg gtgatgctgc tgctgcccgc
    12841 ggcaacgcac gagattgcgg gaccggtgct ggcgctctcg agcacgctcg ccggcaacct
    12901 gctcgtggtg ggaagcatcg ccaacatcat cgtgatcggc gccgcgcagc ggcatcacgt
    12961 gctgatcggg tggacagagc acgcgcgcgt cggcgtgccg gtcacgctcg ccacgatagc
    13021 gatcgccgcg gcatggctgt ggttcagatt gcagggataa cgtaatacac gagttggcag
    13081 ccgtcagcag ttggccgtca gttttcagcc gccagccgtc agcccgctgg aacggcgtga
    13141 acggtgaata gtgaatggtg aatggagctg aagctgggct ccttctgcct tcatccttct
    13201 gccttctgcc ttagttcctc atccctcatc cctcctccct catcccttgg aatggaagct
    13261 tcaaacctaa caatttgtta ggtttgacgg ctgccatttc gcccccgtac cgctaattga
    13321 aacgcgggcg tgatcgtcat cgcaaattcg ttgacagcgc tatctccccg ccctaacgtg
    13381 attgcgctcg gaatttctcg cggcgacccg accacacgga tggaggagag gacatgcggg
    13441 catccaattc caagggggca gtcaaggcac tcgcggttgc cggaactcgc gttgtggccc
    13501 tcgggtggga catgtcggct gcggacatca aatccaggaa ggtcctgggc tttgccatcg
    13561 agcgcacgcg caagcgcgac ggtgaagtga tatggatgcg cggcacgaaa acgttcgagt
    13621 ccgttcttaa agatcccggc cagggcgtaa ccgtatccac ccgggatcat cccttccagt
    13681 cattccagtg gtccgactat tccgtcgagc ccgatcagac ctatacctat cgtatcgtcg
    13741 caaggaccgg cacgccgggc ggtttgaagg acgggccgtc cgcgaagcta gaagtgacta
    13801 ccgaaagcga agaaatcgga aagcacatgg ttttcttcaa ccgcgggtcc gtcgcttccc
    13861 aggagtacgc gcggcgattc aagaacaagt cgccggaggt ggtcggtcag gcggcttggg
    13921 actggctgtc gcgcgggctc atcgaggcac tcgaaaaatt catcggacag gcgggcgaag
    13981 gcgacgaaat tcacggcgcc atcttcgagt tcaagcacaa gcgcgtcttt gacgccctga
    14041 aagccgcgaa aaagcggaaa gccaaaattt tcatcatcta tgacggtcag acccagggtt
    14101 catcgaaccg gaaggaactc aacgccaacg gcgtattcaa ctggctcaag gaagacacgc
    14161 aaatccgcgc ccgcaagcac agtggccggt acgcgcacaa caaatttttt gttctttcgc
    14221 gcaatggaac ggcgaaacag gtctggaccg gctctacgaa tctgagcgtc aacggcatat
    14281 ttggccactc aaacaacgcc cacgtcgttc gtgacgagaa ggtggcgcgg gctttcaggg
    14341 cgtactggga gcggctgtgg gacgacccga ccaagaagcc gcttgccacg gaagatgcga
    14401 agaagacgcc cgcgccgccg ccgcaatggc tcgatgagac ggtgcccatg ttctcgcccc
    14461 agcccgacgt gtcgccgctg gattggtacg ccacgcttgc aggcaagccg aaaaaaccgc
    14521 tcttcacgac ctttgccttc ggaatgaacg agaagttcgt gccgatctac gggcggcgcg
    14581 acggcgtgct tcgcttcgcc ctgatggaaa agaaaggcaa tggcaggcag atcaaggaac
    14641 agtcaagggt catcgacaga atccggcgcc tgccgaacac ggtggtcgca gtgggtgacg
    14701 cggtcgataa ggacaatacc ttcgaccgat ggctcgtcga gcgggtcaag atcgtgaatg
    14761 aagcgcatgt gctcttcgtt cataccaaat acatgctcat cgaccccttg ggtaaggacc
    14821 cgatcgtcat cgtcggatcc gccaacttct ccaaggccag ctgcgatacc aatgacgaaa
    14881 acatgctgat cattcgcggc aacgcgaccg ttgccgacat ctatctcggc gagttcatgc
    14941 ggttgcacac gcattacgcg tatcgcgagt cgctcgggtt ccgccgcacg tcggagaagg
    15001 actacgagag gcggcgcaag tacctcgtgg agtccatcga ctggatcaag ggggaggccc
    15061 agggtcaggg ctatttcgac aagggcggcg cgcgcgccct tcgtcgcgcc tacttttcgg
    15121 gcgggtagcg tttccgccct catcccctaa tcgggctgtg aaaaccagga cgtacgcata
    15181 accacacacg cttcaatatg cgtcgacgca tctggctgca aagcttttgg tattctttgg
    15241 gaatgaactg tatataggag gtctactctg ttcatttcaa aagacccacc tcgcgcgagc
    15301 ttttttgccg gcatggacgt ttggcgcggg ggagagcaaa aaacctaaag gcgcgaggca
    15361 gtaagagtcg aatcacgatt cgcgactcat gcttttttcc gcactctgag ttcggtaccc
    15421 agctattcca agatgaaacg cctcgtcatc tgctgcgacg gcacgtggaa cacgccggac
    15481 cagaaggacc gcggggtgat gcggcccacc aacgtggtga agctggcgcg ctgggtgatg
    15541 ccgcaggcgc ccgacggcac cgtgcagcgc gtccattacg accgcggcgt cgggaccggg
    15601 gacatcatag atcgggcgtt tggtgggatg ttcggcatcg gcctggcgca taacgtccgc
    15661 gaagcgtacg agtttctttc gcagaattac gacgacggcg accaggtctt cctgttcggc
    15721 ttcagccgcg gggcttatac cgtgcgcagg acagtgggca tggtgcgcaa gtgccagttg
    15781 ttgccaaagg atctcggcga cgagacacgc aaggcggcga tcgaggaggg ctacgagatc
    15841 tacacccgcc gcgagggcga gaagcagggc ggcccggact cggaggcggc gcaggcgttc
    15901 cgcaggaagt acggatgccg gccagtcacc atccgctgcc tcggcgtatg ggacaccgtc
    15961 ggcgcctacg gcatcggcgg cgtcctcggc cagctcacca gcagcttcac caaggcgcgc
    16021 tttcacgacc ggcgcctgag ctcgatcgtg gagcatgcct gccaggcggt ggcgatagac
    16081 gaaacacgcc ggctgttcca gcccaacctg tttgagcagg ggcctgccgg gctgacgaag
    16141 ggccaggtgc tcgagcaatc gtggttcgcc ggcgtgcaca gcaacgtcgg cggcggctac
    16201 gaggacacgg ggctgtccga cgtcactctg cactggatgg ccatgcgcgc ggagcagtgc
    16261 gggctcgccc tcgatccgaa gtggcgcgac cggatagacc cggacgagtt cggcgagttg
    16321 cgcgagtcac ggacgggctt gtacctgctc atgggcaagg cggtgcggcc gatcggccgt
    16381 cagaagaacg gcttcgagaa agcccacaac gcgacgatgg accgcatggc gcgcgacccg
    16441 gcgggcta
//
LOCUS       DBNGWZ010000269         3674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1668089, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000269 DBNGWZ010000000
VERSION     DBNGWZ010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1668089"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="AC16CX_07990"
     CDS             complement(<1..133)
                     /locus_tag="AC16CX_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3012488.1"
                     /translation="MEWFRIQRDIPFMRHALVFNVISIVTFLVAVGSLATRGLNLGVD
                     "
     gene            complement(249..2114)
                     /gene="secD"
                     /locus_tag="AC16CX_07995"
     CDS             complement(249..2114)
                     /gene="secD"
                     /locus_tag="AC16CX_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR3012489.1"
                     /translation="MNRYSLWQYLLIAGSLVLGFIYTLPNFFGEVPAVQVSPLKATLK
                     ADTALMGRVEDALKKNNITTSGMFLDATGVKVRFADTDTQLKAKDLLQAQLGEDYIVA
                     LNLLSNSPRWLTAIGALPMYLGLDLRGGVHFLLQVDMKAALAKKMEAFANDVRNGLRE
                     KRIQYTGIARDGQRITVRFREQPMRDKARDELSRTMPDLELKDAEDAGEFRIIATLKP
                     EAQRKTQDFALQQNITTLRNRVNELGVAEPIIQQAGADRVVVQLPGVQDTAKAKDILG
                     RTATLEVRMVDDENTNSPAAVQAALAGQIPFGDELYMERGGVPILVKRQVVLTGDRIN
                     DAQPGFDNQTGEPAVHINLDGTGARIFQQVTRENVGKRMAILLFEKGKGEVVTAPVIR
                     TEIPGGRVQISGRMTTQEARDVALLLRAGALAAPMEIIEERTVGPSLGAENIRIGFHS
                     TWYGFAAIAVYMIAYYMLIGVFSVVALAANVLFLVALLSMLQATLTLPGMAGIALTIG
                     MAIDANVLINERIREELRGGNSPQAAIAAGYDRAWGTILDSNITTLIAGLFLFLLGSG
                     PVKGFAVVLCLGILTSQYSSVMVSRAMVNLYYGSRRRLDKVSIGQVWRPQATTKT"
     gene            complement(2195..2527)
                     /gene="yajC"
                     /locus_tag="AC16CX_08000"
     CDS             complement(2195..2527)
                     /gene="yajC"
                     /locus_tag="AC16CX_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3012490.1"
                     /translation="MLINPAYAQASGAPAGVGGGIESMILIVLMFGVLYFLMIRPQMK
                     RAKEHKTMIDALQKGDEVVAAGGVLGRISKINENYVTLQVSDDVEIQVQRPAVQLVLP
                     KGTLKSIQ"
     gene            complement(2589..>3674)
                     /gene="tgt"
                     /locus_tag="AC16CX_08005"
     CDS             complement(2589..>3674)
                     /gene="tgt"
                     /locus_tag="AC16CX_08005"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3012491.1"
                     /translation="ATDGAARRGKLTLPHGVVDTPAFMPIGTYGAVKGMSPQELKETG
                     AQIVLGNTFHLWLRPGLDVIAAHGGLHRFMGWDGPLLTDSGGYQVYSLGPLRKITEEG
                     VRFQSPVNGDPCFLTPEESMRIQRALGADIVMVFDECTPYPADFGHARDSMQLSLRWA
                     ERSKRAHEGNGNALFGIVQGGMHERLRDESLAGLQGIGFDGYAIGGLSVGETKREMMR
                     VLKHTAPQLPAGRPRYLMGVGTPQDIVAAVAAGIDLFDCVLPTRNARNGWVYTRHGVI
                     KLRNARYRDEVKPLDEECACYTCRNFTRAYLHHLQRVNEMLGARLNTLHNLHYYQELM
                     RELRTAVEQRRLAGFVSESRQRARVES"
BASE COUNT          599 a         1207 c         1210 g          658 t
ORIGIN      
        1 aatccacgcc gaggttgagg ccgcgcgtgg cgagcgagcc cacggcgacc aggaacgtga
       61 cgatggatat cacgttgaac accagcgcat ggcgcatgaa cgggatgtcg cgctggatgc
      121 ggaaccattc catggctacg ctcccgctcc gcgaaggcgg aaggcggaag gcggaagacg
      181 aaagtagcag gctcccgcag tgcgggacac tctcctgact ttcgcctttc gactttcgcc
      241 tttcgccttc atgttttcgt ggtcgcctgc ggccgccaca cctggccgat cgagaccttg
      301 tcgagccgcc ggcggctgcc gtagtagaga ttgaccatgg cccgcgagac catcaccgaa
      361 ctgtactggg aggtgaggat gccgaggcac agcaccacgg cgaagccctt caccggcccg
      421 gagccgagca ggaacaggaa aagacccgcg atgagcgtgg tgatgttgga atcgagaatg
      481 gtgccccagg cgcggtcgta accggcggcg atcgccgcct gcggcgagtt gccgccgcgc
      541 agctcctcgc ggatgcgttc gttgatcagc acgttggcgt cgatcgccat gccgatcgtc
      601 agcgcgatgc cggccatgcc gggcaaggtc agggtggcct gcagcatcga gagcagcgcc
      661 accaggaaca gcacgttcgc ggcgagcgcg acgaccgaga acaccccgat cagcatgtag
      721 taggcgatca tgtacaccgc gatcgcggcg aacccgtacc aggtggagtg gaagccgatg
      781 cggatgttct ccgcgccgag cgagggtccg accgtgcgct cctcgatgat ctccatcgga
      841 gcagcaagcg ccccggctcg cagcagcagc gcgacgtcgc gcgcctcctg cgtcgtcatc
      901 cgaccgctga tctgcacccg cccgcccgga atctcggtgc ggatcacggg agcggtcacc
      961 acctcgccct tgcctttctc gaacagcagg atcgccatgc gcttgccgac gttctcgcgc
     1021 gtgacctgct ggaagatgcg cgcgccggtg ccgtcgagat tgatgtgcac cgccggctcg
     1081 ccggtctggt tgtcgaagcc cggctgcgca tcgttgatgc ggtcgccggt cagcaccacc
     1141 tgcctcttca ccaggatcgg cacgccgccg cgctccatgt agagttcgtc gccgaacgga
     1201 atctggcccg ccagcgccgc ctggaccgcc gcggggctgt tggtgttctc gtcgtccacc
     1261 atgcgcactt ccagcgtcgc cgtgcgcccg aggatgtcct tcgctttcgc ggtgtcctgc
     1321 acgcccggca gttgcaccac cacgcggtcc gcgcccgcct gctggatgat cggctccgcg
     1381 acccccagct cgttgacgcg gttgcgcagc gtggtgatgt tctgctggag cgcgaagtcc
     1441 tgggttttcc tctgcgcctc gggcttgagc gtggcgatga tgcggaattc gcccgcgtcc
     1501 tccgcgtcct tcagctcgag atcgggcatg gtgcgcgaca gctcgtcgcg cgccttgtcg
     1561 cgcatcggct gctcgcggaa acgcaccgtg atcctctggc cgtcgcgcgc gatgccggtg
     1621 tactggatcc gtttctcgcg caggccgttg cggacgtcgt tggcgaaagc ctccatcttc
     1681 ttggcgaggg ccgccttcat gtccacctgc agcaggaagt gcacgccgcc gcgcaggtcg
     1741 aggccgaggt acatcggcag cgcgccgatc gcggtgagcc agcgcgggct gttcgaaagc
     1801 aggttcagcg cgacgatgta gtcctccccg agctgcgctt gcagcaggtc cttggccttc
     1861 aactgggtat cggtgtcggc gaagcgcacc ttgacgccgg tcgcgtcgag aaacatgccg
     1921 cttgtcgtga tgttgttctt cttgagcgcg tcctcgacgc ggcccatgag cgcggtgtcg
     1981 gccttgagcg tcgccttgag cggcgagacc tgcaccgccg ggacctcgcc gaagaaattg
     2041 ggcagcgtgt agatgaagcc caataccagc gagcccgcga tgaggaggta ctgccagagg
     2101 gagtagcggt tcatgaataa tcgtgaggcg tgataggtga ggagtgaggc gagcgaatca
     2161 cgagttttcc gtctaacgcc ttacgcctaa cgcctcactg gatgcttttg agagtgccct
     2221 tcgggagcac gagctgcacc gcgggacgct ggacctggat ctcgacgtcg tcggacacct
     2281 ggagcgtcac gtagttctcg ttgatcttgc tgatgcggcc gagcacaccg ccggcagcga
     2341 ccacctcgtc tcccttctgc agcgcgtcga tcatggtctt gtgctccttg gcgcgcttca
     2401 tctgcgggcg gatcatcaga aagtagagca caccgaacat cagcacgatc aggatcatgc
     2461 tctcgatgcc cccgccgact cccgcgggag cgccggaggc ctgggcatag gccgggttga
     2521 tcaacatgaa attctccgtt ggttgcggcg gggaggcgcg tcatgcctct gaaaaggcgg
     2581 gcattttatc acgattccac gcgcgcccgt tgcctggatt cggaaacaaa tccggcgagc
     2641 cgcctctgct cgacagcggt ccgcagctcg cgcatcaact cctgatagta gtgaaggttg
     2701 tgcagcgtgt tgagccgcgc gcccagcatc tcgttcacgc gctgcaggtg gtgcaggtag
     2761 gcgcgcgtga agttgcggca ggtatagcag gcgcactcct cgtcgagcgg tttgacctcg
     2821 tcgcgatagc gcgcgttccg cagcttgatg acgccgtgac gcgtgtacac ccagccgttg
     2881 cgcgcgtttc tcgtcggcag cacgcaatcg aaaagatcga tgccggcggc gaccgccgcc
     2941 acgatatcct gcggcgttcc gacgcccatc aggtaacgcg gccgcccggc gggcagttgc
     3001 ggcgccgtat gcttgagcac ccgcatcatc tcgcgcttgg tctcgcccac cgacagcccg
     3061 ccgatggcgt agccgtcgaa tccgattccc tgcaagccgg cgagtgactc atcgcggagc
     3121 cgctcgtgca tcccgccctg cacgatgccg aagagcgcgt tgccgttgcc ctcgtgcgcg
     3181 cgtttggagc gctctgccca gcgcagggaa agctgcatcg aatcgcgcgc gtgcccgaaa
     3241 tcggcgggat acggcgtgca ctcgtcgaac accatgacga tgtcggcgcc gagcgcgcgc
     3301 tggatgcgca tggactcctc gggcgtgaga aagcacgggt cgccgttgac cggggactgg
     3361 aatctcacac cctcttcggt gatcttgcgc agcggcccga ggctgtacac ctggtagccg
     3421 ccggagtcgg tgaggagcgg gccgtcccag cccatgaagc ggtgcaggcc gccgtgcgcg
     3481 gcgatcacgt ccaggcccgg gcgcagccag agatggaagg tgttgccgag cacgatctgc
     3541 gcgccggtct ccttcagctc ctgcgggctc attcccttca ccgcgccgta ggtgccgatc
     3601 ggcatgaacg caggcgtgtc caccacgcca tgagggagcg tgagcttccc gcgccgcgcc
     3661 gcgccgtcag tcgc
//
LOCUS       DBNGWZ010000270         2315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1917067, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000270 DBNGWZ010000000
VERSION     DBNGWZ010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1917067"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC16CX_08010"
     CDS             <1..367
                     /locus_tag="AC16CX_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012492.1"
                     /translation="ISNVSTAVPHVKSGRLRLLAVTTGKRFPLFPGTPTVSEAGVPGY
                     ESSGWYGMWVPAETPPSVIKTLNGEMMKILNSAAINEQFGAQGLQPIPVSPETFGRRL
                     REEIRKWGGVVGASGVKPE"
     gene            complement(553..1209)
                     /locus_tag="AC16CX_08015"
     CDS             complement(553..1209)
                     /locus_tag="AC16CX_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3012493.1"
                     /translation="MKPVHLLLCAFALLPALTPAQQPVFQPAPQPAAGRSALLVKPPS
                     GPELLTELRKGGYIIYFRHTSTDFSRDDTRSTSDDDCDNQRPLTDKGREEAKQIGAAF
                     RELKIPLERVLSSPRCRTMETAMLAFGRADKSPDVRGGPAAPDSPDRYAGLRRLLSTP
                     VKPGANLVISSHGNPFYAVAGRPYLAEGEAAVIRPLGNDFEIVARVKHDAWRGLMKRD
                     "
     gene            1433..>2315
                     /locus_tag="AC16CX_08020"
     CDS             1433..>2315
                     /locus_tag="AC16CX_08020"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3012494.1"
                     /translation="MKCAAQLPLSAGAAAAAAPAAVATGVYAGFWKRFAAVILDYIVL
                     IALAAVVGGIAGFIYGTSMSSADQRSAEMLGNIVGLLVWWLYYALMESSGTQATLGKM
                     ALGIKVVDQQGGRISFGRATGRHFAKILSSLILMVGYLMAGFTARKQALHDMVAGCLV
                     VNRSAPEEQIRQGVTEARMPAWAIVLIVLAVCVLPFAVIAAVAIPAYHDYTVRARVAM
                     AVKAGDQATRAVEDFCRRNNTLPRDLKEAGMEAPLAREVKQVNVDPRSGAIQVVLAVS
                     SLEGKSILFEPKRDSENR"
BASE COUNT          441 a          679 c          774 g          421 t
ORIGIN      
        1 catctccaac gtctccactg ctgtgcccca cgtcaaatcc ggcaggttgc ggctgctcgc
       61 ggtgacgacg ggcaagcgct tcccgctgtt tccgggcacg ccgacggtga gcgaagcggg
      121 cgttcccggt tacgagagca gcggctggta cggcatgtgg gtcccggcgg agacaccacc
      181 gtcagtcata aaaacgctta acggggaaat gatgaagata ctgaactcgg ctgctatcaa
      241 cgagcaattc ggcgcgcagg gcctacaacc gatacccgtg tcgccggaga cattcggaag
      301 gcgcctgcgc gaggaaatca ggaagtgggg cggggtggtc ggggcgtcgg gcgtgaagcc
      361 cgagtgatag atcggtgata agtgataggt gtgagtcaaa acagcatccc tcgccccttc
      421 gccctcatcc cctagtactc gccgtcgatg aaccccgata aatgcagata gagatacaga
      481 caataaagcc gcttggtggg gcacccccta tccgttacgc tgtatcagtc acgtcccacc
      541 agtcacgctt catcagtcac gcttcatcag gccgcgccag gcgtcgtgct tcacccgtgc
      601 cacgatctca aagtcattcc caagtggccg gatcactgcc gcctcacctt cggcgagata
      661 gggcctcccg gcgacggcgt agaacggatt gccgtggctg gagatcacca ggttagcgcc
      721 aggctttacc ggagtggaca gcagccggcg cagcccggcg tagcggtcgg ggctgtcggg
      781 cgcagccggc ccgccgcgca catctggcga cttgtccgcg cggccgaaag cgagcatcgc
      841 ggtttccatc gtcctgcaac gcgggctcga cagcacgcgc tcgagcggta tcttcagttc
      901 gcggaatgcg gcgccaatct gcttcgcttc ctcgcggccc ttgtcggtga gcgggcgctg
      961 gttgtcgcag tcgtcgtcgc tcgtcgagcg tgtgtcgtcg cgcgaaaaat cggtcgaggt
     1021 gtgacggaag tagatgatgt aaccgccctt gcgcagttcc gtgagcagtt ccggaccaga
     1081 tggcggtttt accagcagcg cgctgcgccc agcggcgggc tgcggcgcgg gctggaatac
     1141 gggctgctgc gcgggcgtta gcgcaggcag cagcgcaaaa gcgcaaagca gtaaatggac
     1201 aggtttcata aggaatctcc gcgcaaaaaa gcgcgcggag attccttgac aagataaggt
     1261 catgaaggaa cccggtaaat cccgcctgtt attatggctc cccggtgcaa gaattgctcg
     1321 gatgtattgt gagggggcat gatggaaggc aatatgtcaa ttgaggagga gcaggggagc
     1381 catgtattgt ccaaaatgcg gtcaacaaaa cgacgatagc gcgagatttt gcgtgaaatg
     1441 cgctgcacag ctgccgcttt ccgcgggtgc cgcggccgcg gcagcgccgg cggcagtcgc
     1501 gaccggggtc tacgccggct tctggaaacg ctttgcggca gtgatccttg attacatcgt
     1561 cctgatcgcg ctggctgcgg tcgtgggcgg catcgccggc ttcatctatg ggacgagtat
     1621 gagctccgcc gaccagagat ccgcggagat gctgggcaat atcgtcggcc tcctcgtgtg
     1681 gtggctctac tacgcgctca tggaaagctc gggcacgcag gcgacgctcg gcaagatggc
     1741 gctcggcatc aaggtcgtgg atcagcaagg cgggcgtatc tccttcgggc gcgccacggg
     1801 acgccatttc gccaagatcc tgtccagcct gatcctgatg gtcggctacc tgatggcggg
     1861 gttcacggcc aggaaacagg cgctgcatga catggtggcg gggtgcctgg tcgtcaatcg
     1921 cagcgcgccg gaggagcaga tccggcaggg cgttaccgaa gcgcggatgc cggcgtgggc
     1981 gatcgtgctg atcgttctcg ccgtgtgcgt gcttccgttt gcagtcatcg cggcagtcgc
     2041 cattccggct tatcacgatt acaccgtgcg cgcccgcgtg gcgatggcgg taaaggccgg
     2101 cgaccaggcg acccgggcgg tggaagactt ctgtcgccgg aacaacacgc taccccgcga
     2161 tctcaaggag gcgggcatgg aggccccgct tgcccgtgag gtcaagcagg tgaacgtgga
     2221 cccgcgcagc ggtgcgatcc aggtcgtgct ggcggtgtcg tcgctggaag ggaaatccat
     2281 cctgtttgag cccaagcggg acagcgaaaa ccgca
//
LOCUS       DBNGWZ010000271         3417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_911333, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000271 DBNGWZ010000000
VERSION     DBNGWZ010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_911333"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16CX_08025"
     CDS             <1..278
                     /locus_tag="AC16CX_08025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3012495.1"
                     /translation="SRIGYDNLENLKRTRAPVLVAHSRDDDIVPFAHGVRLYEAATGP
                     KAFLEMRGGHNEGFIFARPEWVAQLAAFLDRHNRQESPAPPKPDRQD"
     gene            395..1219
                     /locus_tag="AC16CX_08030"
     CDS             395..1219
                     /locus_tag="AC16CX_08030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3012496.1"
                     /translation="MAKHNPRIGIALGSGSARGWAHIGVLQALAEAGLAPEVVCGTSI
                     GALVGAVYAEDNLAEFERWVKALTWRRVMGYFDLSFSGGILKGGKLFDHLREEFLHGR
                     IEDLKRPFAAVATDLATGREVWLRKGPVAGAVRASIATPGLFTPWPDGERLLVDGGLV
                     NPVPVSVARALEADVVIAVDLSSDLVGRHALAADKDEPTKPPNMIEIVTGSLNIMSVR
                     ITRSRLAGEPADAVIQPRLAEVGLLEYHRGAEAIEEGRAAAELAIPQLRRLLLDRT"
     gene            1250..1651
                     /locus_tag="AC16CX_08035"
     CDS             1250..1651
                     /locus_tag="AC16CX_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3012497.1"
                     /translation="MAEKRFINPEGLTRPGTYTPVVATRGGRTIYVSGQVSVNGKGEL
                     VGKGDLGAQAEQVFHNIETALKGAGATFGDVVKITTFIVGYKPEHRSLMHQVRSRYVS
                     KENPPASTLLGVQALAIADYLIEVEAIAVAD"
     gene            complement(1772..2551)
                     /locus_tag="AC16CX_08040"
     CDS             complement(1772..2551)
                     /locus_tag="AC16CX_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3012498.1"
                     /translation="MADSFSQTAKGKAEFAAKPGKLTPGLKTLLGMIGDKVTIADLKI
                     KLPQVPVDKLTAALDRLVADGYLQRAAVPVRMHSDASLDFTRFINRPVKEPTLQQKRH
                     AEATLAGIRPSKKAGYHVNIVNRPAKRVTPRAGGDKYTVLLIDADEANALIMARALLL
                     AKFDTRAAAEREEIVAELNRQPPPDAVAMDVELPDVIGLELLGRMRDHPVYKTIPIIV
                     MTAKVEHDDVVAALAYGASGFITKPFKPEALLESVRAVLGL"
     gene            complement(2699..>3417)
                     /locus_tag="AC16CX_08045"
     CDS             complement(2699..>3417)
                     /locus_tag="AC16CX_08045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3012499.1"
                     /translation="PEVRCVAGFLSPSTGIVDSHGLMLAYLGDAESCGASLALGSPVV
                     SGRVEGGGIVLEVGGAESTALKCDVVVNSAGLNAQAVARSVAGIPPRTIPPTHYAIGH
                     YYTLAGPAPFRRLIYPVVRRDWLGVHVTIDLGGQVKFGPDFSWIDRIDYRFDESRATA
                     FYSAIRRYYPGLRDGTLQPGYTGIRPKIHGPGEPASDFVIHGPRDHGVAGLVNLYGIE
                     SPGLTSSLAIADHVAALLSN"
BASE COUNT          592 a         1059 c         1160 g          606 t
ORIGIN      
        1 tcagccgcat cgggtacgac aatctggaga acctgaaacg aacccgcgcg ccggtgctcg
       61 tcgcgcacag ccgggacgac gatatcgtgc cgttcgctca cggggtcagg ctctacgagg
      121 cggcgaccgg gcccaaggcc ttcctggaaa tgcgcggcgg ccacaacgag ggcttcatct
      181 tcgcgcggcc cgaatgggtc gcccagctcg ctgcgttcct cgaccggcac aatagacagg
      241 agtcaccggc tcccccaaaa cctgatagac aggattaaca agattcaaac ggacaaaccc
      301 gaacttcttt tagcttggtc tcggtctaat cctgttaatc ttgtaaatcc tgtccattgt
      361 ttttgatcct gagtttttaa tcctgtccgt ttgtatggca aagcataacc cccgcatagg
      421 catcgcgctc ggctccggct cggcgcgcgg ctgggcgcac atcggcgtgc tgcaggcgct
      481 ggcggaagcc gggctcgcgc ccgaagtggt gtgcggcacc tcgatcgggg cgctggtggg
      541 ggcggtttat gccgaggaca atctcgcgga gttcgagcgg tgggtcaagg cgctcacctg
      601 gcgcagggtc atgggctatt tcgacctttc cttcagcggc ggaattctga agggcggcaa
      661 gctgttcgat cacctacgcg aagaatttct ccacggccgc atcgaggact tgaagcggcc
      721 tttcgccgcg gtcgcgaccg acctcgcgac cggccgggaa gtctggttgc gcaaaggccc
      781 ggtcgccggc gcagtgcgcg cctcgattgc aacgccgggg ctgttcacgc cgtggccgga
      841 tggcgagcgc ttgctggtgg acggggggct ggtcaatccg gtgccggtgt cggtcgcgcg
      901 ggcgctggag gccgacgtgg tgatcgcggt ggacctgagc tccgacctgg tcggccgaca
      961 cgcgctcgcc gcggataagg acgagccgac gaaaccgccc aacatgatcg agatcgtgac
     1021 cggcagcctc aacatcatga gcgtgcgcat cacgagaagc cgtctggccg gcgagccggc
     1081 cgatgcggtc atccagccgc gcctggccga ggtgggtttg ctggaatatc accgcggcgc
     1141 cgaagcgatc gaagaggggc gcgccgccgc cgagcttgcg attccgcaac tccggcgcct
     1201 gcttctggac cggacttgaa cgactgtctt gattggaagg aggggaacca tggcggagaa
     1261 gcgattcatc aatccggaag gactgacccg gcccggcacc tatacgcccg ttgtcgccac
     1321 ccgcggcggc cggacgattt acgtttcggg acaggtgtcg gtgaacggga agggcgagct
     1381 ggtgggcaag ggcgatctcg gggcgcaagc ggagcaggtc ttccacaaca tcgaaacggc
     1441 gctcaagggc gccggcgcga cgttcggcga tgtcgtcaag atcaccacct tcatcgtcgg
     1501 ctacaagccc gaacaccgca gcctgatgca ccaggtgcgg tcgcgctatg tgtcgaagga
     1561 aaacccgccc gcaagcacgc tgctgggcgt gcaggccctg gcgatcgcgg actacctgat
     1621 cgaagtcgag gcgatcgccg tcgcggactg acgtcgaagg cgaaaggcga aaggcgaaag
     1681 gcgaaagtag ttaggcggaa gacgggaagc gaagaagggt tgacccaggg gttttccctt
     1741 ccgccttttg cctttcgact ttcgccttgc tttagagccc gagcacggcc ctgacgcttt
     1801 cgagcaacgc ctcgggcttg aacggcttgg taatgaagcc gctcgcgccg taggcgagag
     1861 ccgccaccac gtcgtcgtgc tcgaccttgg cggtcatgac gatgatcgga atcgtcttgt
     1921 agacggggtg atcgcgcatg cggccgagca gctcgagccc gatcacgtcg ggcagctcca
     1981 cgtccatggc gacggcatcg ggcggcggct gccggttgag ctcggcgacg atctcctcgc
     2041 gctccgcggc ggcgcgggtg tcgaacttcg cgagcagcag cgcgcgcgcc atgatcagcg
     2101 cgttggcctc gtcggcgtct atgaggagca cggtgtattt gtcgccgccg gcgcgcggcg
     2161 tgacgcgctt ggcgggccgg ttgacgatat tgacgtgata acccgccttc ttggaagggc
     2221 ggatgccggc cagggtcgcc tcggcatgcc gcttctgctg cagcgtcggc tccttgaccg
     2281 gccggttgat gaagcgcgtg aagtcgaggc tcgcgtcgga gtgcatgcgc acgggcacgg
     2341 ccgcgcgctg gagatagcca tcggcgacca gcctgtcgag ggcggcggtc agcttgtcca
     2401 cgggcacctg cggcagcttg atcttcaggt ccgcgatcgt cactttgtcg ccgatcatgc
     2461 cgagcagcgt cttcaatccg ggcgtgagct tgccgggctt ggccgcgaat tcggccttgc
     2521 ccttcgcggt ctggctgaag ctatcggcca ttgtcttgcg ccctttttcc ggtgggcgtg
     2581 aaatgagcgt cgctttggcg tccgctcaat ttcgcgcgat tattccgggg cttagcatcc
     2641 ggaatgacga aagattatgc cacgcgcatt ccccgcccgc ttgacgtgaa tcaactattc
     2701 agttgctcag caacgccgcc acatgatcgg cgatggcgag cgaagaagtg aggcccggcg
     2761 attcgatgcc gtagagattg acgagccccg cgacaccgtg atcacgcggc ccatgtatca
     2821 cgaagtcgga tgccggttct ccaggtccgt ggatcttggg ccggatgccg gtatagccgg
     2881 gctgcagcgt cccgtccctc aaccccggat agtagcggcg gatcgcgctg taaaacgccg
     2941 tcgcgcggct ttcgtcgaag cggtagtcga tgcgatcgat ccacgagaaa tcggggccga
     3001 acttgacctg tccgccgaga tcgatggtga cgtgcacccc gagccagtcc cggcgcacga
     3061 ccgggtagat cagccgcctg aagggagccg ggccggcgag ggtgtaataa tggccgatgg
     3121 cgtagtgggt cggcgggatc gtacgcggcg ggatgccggc gaccgagcgc gccaccgctt
     3181 gggcgttgag gccggcgctg ttcaccacga cgtcgcactt gagcgccgtc gattccgcgc
     3241 cgcccacctc gagcacgatg ccgccgccct ccaccctgcc cgagacgaca gggctgccga
     3301 gtgcgagcga ggcgccacag ctctcggcat ctccgagata ggcgagcatc agcccgtggc
     3361 tgtccacgat gccggtggag ggcgagagaa agccggccac gcaacgcacc tctggct
//
LOCUS       DBNGWZ010000272         5024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_657861, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000272 DBNGWZ010000000
VERSION     DBNGWZ010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_657861"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1120)
                     /locus_tag="AC16CX_08050"
     CDS             complement(11..1120)
                     /locus_tag="AC16CX_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012500.1"
                     /translation="MNSDRETIRQPALAEERAALFEAPDDVEAINRLYRERRWNDGLP
                     IVPPTVERVGRMLVHARRGRDEVVARIAPGYGAATVERIAINAVMAGCDPEYLPIVSA
                     AVEAVAAPEFNLQAIQATTNPVAVWIIVNGPAGKRLGVNGTYNCLGQGTWANATIGRA
                     LRLVLQNVGGATPGEMDRATHGQPGKYTFCCAENEEANPWEPLHVERGLRREASTVTV
                     VGAEGTMNMNTHTKDAGELLRVIAETMQHPPSNEYCYGSEPWLILSPEHAEILKAGGL
                     GKTEVKRRLWELSKMPARRMTARDLLRVQAARGKELGEIRPDTLLSIAKRPEDICIIV
                     AGGPGTHSVYVPCFGNTRSVTREIADVDPTADKRR"
     gene            complement(1110..1649)
                     /locus_tag="AC16CX_08055"
     CDS             complement(1110..1649)
                     /locus_tag="AC16CX_08055"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1368..1370),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3012501.1"
                     /translation="MVLLYDPTAPRRAEAANAGGKVDTLAGKVVGFIDNAKPNFNHLV
                     DDLAELLVARCGVKHVIKRRKQSASVPAPDEVVRELADECDVVIAGSGDUGSCASWSV
                     HDSVEIAKRGRAAVTICSTAFITLGRAQARALGNPELPIAVIPHPFGSRTRDEVRELA
                     RECVTDVVALITQKTPDEQ"
     gene            complement(1793..2761)
                     /locus_tag="AC16CX_08060"
     CDS             complement(1793..2761)
                     /locus_tag="AC16CX_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012502.1"
                     /translation="MRVILILFAAWLCGVQSNTWSAEAYPTRAIRLIVPYPPGGAGDI
                     VGRMLAARFTEALGQQVVVDNRGGGGQIIATELAAKAPPDGHTLFLASATHGINPGLR
                     RGKLPYDTLKDFAPITLVAESPLVFVAHPSLGVGNIKELIAVAKARPGRINYASSGPG
                     TGGHLSVELLKFQTGIDLVHVPYKGAGPALTDLIGGQVQMMCTSPLPAMPHVKSGRLR
                     ALAMTGRSRSRAAPDVPTVAEQISTRYESSLWYALLAPAATPQPVIKRVHAETVKIIK
                     SADVSEQLLAQGAEPVGNTPPELQKFISAEIKRWTEVVEKANIRAD"
     gene            2861..4264
                     /locus_tag="AC16CX_08065"
     CDS             2861..4264
                     /locus_tag="AC16CX_08065"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR3012503.1"
                     /translation="MEEIRILSPTGVLGSGFLETSFEAALARKPHFIGCDAGSTDPGP
                     SHLGSGDPAFPRAAVKRDLRLMLGGARRLNVPLLIGSAGTAGADAHLAWTFDILKEVA
                     AEEKLAFRLALIHAEQDKAYLKRRLREGRIKPLHPAPPFDEQVIERAERIVGMMGAEP
                     FMRALEAGADVIIAGRASDTAIFAALPVMRGFPEGLAWHAAKILECGAAAVVNRKTPD
                     CMMAFLRKDHFVVEAPDPGLRCTPQSIAAHSLYENADPFRLVECSGTLDLTHSRYEAV
                     DGRAVKVSGSEFVPAPRYTVKLEGAEKIGYQSVVIGSVRDPYIIRQIDDWTARLQDRI
                     HARVAQVFGAALKREEYDIRIRIYGKNGTMGPLEPVKEIRSHELALLIETTAPTQETA
                     SSIAGIVRHQALHLPIPEWSGLITALACPYNPAHLDRGAVYRFNVNHVVEPDDPCEMF
                     PMELYKVNGPAITEVQK"
     gene            4261..4569
                     /locus_tag="AC16CX_08070"
     CDS             4261..4569
                     /locus_tag="AC16CX_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HIR3012504.1"
                     /translation="MITLARLARLIRSKNAGPFELTFDIMFDDAATYRRVRESGALNR
                     NVVAACYGLEPGQVKFFYCDNALAVKASIPRPHAQGDLGDADGHGGQQYAPLMDIEIP
                     "
     gene            4748..>5024
                     /locus_tag="AC16CX_08075"
     CDS             4748..>5024
                     /locus_tag="AC16CX_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012505.1"
                     /translation="MKITALRFTAIAIVTTLASSLTGVYAMAQSYPTKPIRLIVPFAP
                     GGGTDITARAVAQKLSESWGQAVVADNRPGANGTIGVDITAKSAPDGY"
BASE COUNT          856 a         1655 c         1605 g          908 t
ORIGIN      
        1 ttatccgcgt ttatctgcgt ttatctgcgg ttggatctac gtctgcgatc tcgcgggtga
       61 cggagcgtgt gttcccgaag caggggacgt aaacggaatg cgtgcccggg ccgccggcca
      121 cgatgataca gatgtcttcg ggccgcttcg cgatggagag cagagtatcg ggccggattt
      181 cacccagttc cttcccgcgc gcggcctgta cccgcaggag atccctggcg gtcatgcgcc
      241 gcgcgggcat cttcgacagc tcccaaaggc ggcgcttcac ctccgtcttg ccgagcccgc
      301 ctgccttgag tatctccgcg tgctcgggcg acaagatcag ccagggctcg ctgccgtagc
      361 aatactcgtt gctcggcgga tgctgcatcg tctcggcgat gacccgcaac agctcgcccg
      421 catccttggt gtgggtgttc atgttcatgg tgccctcggc gcccaccacc gtcaccgtgc
      481 tcgcctcgcg ccgcagacct cgctccacgt gcagcggctc ccacggattc gcttcctcgt
      541 tttcggcgca gcagaaggtg tacttgcccg gctgcccgtg ggtcgcgcga tccatctcgc
      601 ccggagtcgc accgccgacg ttctgcaaca ccaggcgcag cgcacgtccg atcgtcgcgt
      661 tcgcccaggt tccctgcccc agacagttgt aagtgccgtt gacacccagc cgcttgccgg
      721 cggggccgtt gacgatgatc cagacggcaa cggggttcgt cgtggcctgg atcgcctgca
      781 ggttgaactc gggcgcagcc accgcctcga cggcggcgct gacgatcggc agatattcgg
      841 gatcacaacc cgccatcacg gcgttgatgg cgatgcgctc cacggtcgcc gcgccgtagc
      901 ccggggcaat ccgggcgacg acttcgtcgc ggccccgacg cgcgtgcacc agcatccgcc
      961 cgacgcgctc gacggtgggc ggaacgatgg gcagaccgtc gttccatctg cgctcgcggt
     1021 agagacggtt gatcgcctcg acgtcgtcgg gcgcctcgaa caaggcagcc cgctcttcgg
     1081 caagcgcggg ctgcctgatg gtttcgcggt cactgttcat cgggagtctt ttgggtaatc
     1141 agtgccacga cgtcagtcac gcattctctt gccagctcgc gcacctcgtc ccgcgtgcgc
     1201 gagccgaagg gatgcgggat caccgcgatc ggcagctccg ggttcccgag cgccctcgcc
     1261 tgcgcgcgcc cgagcgtgat gaaagcggtg gaacagatgg tgacggccgc gcgcccccgt
     1321 ttcgcgattt ccacactgtc gtggacactc cacgacgcgc acgaccctca gtcgcccgac
     1381 ccggcgatca ccacgtcgca ctcgtcggcg agctccctca ccacttcgtc gggcgcggga
     1441 accgaggccg attgctttct gcgcttgatg acgtgcttta cgccacatct tgcgaccagc
     1501 agctcggcga gatcgtccac gaggtgattg aaattcggct tggcgttgtc gatgaagccg
     1561 acgaccttgc cggcgagcgt gtccaccttc ccgcccgcgt tggccgcttc agcccgccgc
     1621 ggcgcggtag gatcgtagag caacaccatc tcgttctcct aggaaatcat taaaccgccg
     1681 atgaacgccg atgaacgcaa ataattttaa gacaaccgat tgtttctatt ctatatcggt
     1741 gttaatcggc gtttatctgc ggttaattct gtttttgatc ttgatgcgaa ttttaatctg
     1801 cgcggatatt cgccttttcg acgacctcgg tccagcgttt gatttccgcc gaaatgaact
     1861 tctgcagttc cggcggtgtg ttgccaaccg gttccgcgcc ctgggcgagg agctgctctg
     1921 acacatcggc ggacttgatg atcttcacgg tctcggcatg cacccgcttg atgaccggct
     1981 gcggcgtggc ggccggcgcg agcagcgcgt accacaatga tgattcgtag cgcgttgaga
     2041 tctgctcggc gacggttgga acgtccggcg ccgcgcgcga acggctccgg ccggtcatcg
     2101 cgagcgcccg cagcctcccc gacttgacgt gcggcatggc gggcagcggg ctggtgcaca
     2161 tcatctggac ctggccgccg atcaggtcgg tgagcgccgg tcccgcgccc ttgtagggca
     2221 cgtgcaccag atcgatgccc gtctggaact tcagcaactc gaccgatagg tggccgccgg
     2281 tgccgggacc cgaggaggcg tagttgatgc ggccgggcct cgccttcgcc accgcgatca
     2341 gctccttgat gttgccgacg ccgagcgacg gatgcgcgac gaagacgagc ggcgactccg
     2401 cgaccagcgt gatcggcgcg aagtccttca gcgtgtcgta gggcagcttc cccctgcgca
     2461 ggccgggatt gatgccgtgc gtcgcgctcg cgagaaacag cgtgtggccg tcgggcggcg
     2521 ccttcgccgc cagctcggtt gcgataatct ggccgccgcc gccgcggttg tcgacgacga
     2581 cctgctggcc cagcgcctcg gtgaatcttg cagccagcat ccggcccacg atgtccccgg
     2641 ccccgccggg cggatacggc acgatgagcc ggatcgcgcg cgttggatag gcctcagcgc
     2701 tccacgtgtt ggactgaacc ccgcaaagcc atgccgcgaa caggatcaga atgacgcgca
     2761 tagtcgatat actatatttg gcgcgggctg atcaccagcc acgaacccac ggcgtcagtt
     2821 gattgacggg attgtgggag aaccaacccg cgccaaatcc atggaagaaa ttcgcatcct
     2881 gtcgccgacc ggcgtgctcg gttcgggctt cctcgagacg tcgttcgaag cggcgctcgc
     2941 ccgcaagccg catttcatcg gttgcgacgc gggctcgacc gatcccggcc cgagccacct
     3001 cggctcgggc gacccggcct tcccgcgcgc cgcggtgaag cgcgatttgc ggctcatgct
     3061 cggcggcgcg cggcgtctga acgttcccct gctcatcggc tcggcgggca ccgccggagc
     3121 cgacgcgcat ctcgcgtgga ccttcgacat cctcaaggaa gtcgcggccg aggagaagct
     3181 cgcattccgg ctcgctctga ttcacgccga gcaggacaag gcatacttga agcgccgcct
     3241 gcgcgaggga cgcatcaagc cgcttcatcc cgcacccccg ttcgacgagc aggtcatcga
     3301 gcgcgccgag cgcatcgtcg gcatgatggg tgccgaaccc ttcatgcgtg cgcttgaggc
     3361 gggggccgac gtcatcatcg cggggcgcgc gagcgacacc gcgatcttcg cggcgctgcc
     3421 ggtgatgcgg ggctttcccg aaggccttgc ctggcacgcg gccaagatac tggagtgcgg
     3481 cgcggcggcg gtggtcaacc gcaagacacc tgactgcatg atggcgttcc tgcgcaagga
     3541 tcactttgtg gtggaggcgc cagacccggg gctgcgctgc acgccgcaga gcatcgccgc
     3601 gcacagcctg tacgagaacg ccgatccctt caggctcgtg gagtgctccg gaacgctcga
     3661 tctgacgcat tcccgctacg aggcggttga cggccgcgcg gtcaaagtat ccggcagcga
     3721 gttcgttccg gcgccgcgct atacggtcaa gctcgagggc gccgagaaaa tcggctacca
     3781 gagcgtcgtg atcggctcgg tgcgcgatcc ctacatcatc cgccagatcg atgactggac
     3841 ggcgcggctc caggatcgta ttcacgcgcg ggtggcgcag gtgtttggag ccgcgctcaa
     3901 gcgcgaggag tacgatatcc gtatccgcat ctacggcaag aacggcacca tgggcccgct
     3961 cgagccggtg aaggagatcc gctcgcacga gctcgccctg ctgatcgaga cgaccgcgcc
     4021 gacgcaggag accgcgagct cgatcgcggg catcgtgcgc caccaggcgc tgcacctgcc
     4081 gatccctgaa tggagcgggc ttatcaccgc gcttgcctgc ccctacaacc cggcgcatct
     4141 cgaccgcggc gcagtctacc gcttcaacgt caaccacgtg gtcgagcccg acgatccctg
     4201 cgagatgttc ccgatggagc tttacaaggt taatgggccg gccatcaccg aggtgcaaaa
     4261 gtgatcacgc tcgcccgact ggcacggctc atacgcagca aaaatgccgg cccattcgag
     4321 ctcacgttcg acatcatgtt cgatgacgcg gcgacgtatc gccgcgtcag ggaatcgggc
     4381 gccctgaacc ggaacgtagt cgcggcctgt tacggcctcg agccgggaca ggtcaagttc
     4441 ttttactgcg acaacgccct cgccgtgaag gcgagcattc cgcggccgca cgcccagggc
     4501 gacctggggg acgcggacgg gcatggcggg cagcaatatg ccccgctgat ggacatcgaa
     4561 atcccttaaa gccggacgca gggcgccggt ttaagttcgc gtgttcgaac ggacaaaatg
     4621 accgccgttc aacacgcggg atttagagta tccgagggca gcgcgagggc ggactgaacg
     4681 gcttcggaaa cgttggtggg aacggcatta gaataggtgc acctaaagac atcgggagaa
     4741 gccggtcgtg aaaatcacag ctttgcgctt tactgcaatt gccatcgtga cgacgctggc
     4801 ctcttcgttg acgggtgtct atgcgatggc gcaaagctat ccgacgaagc cgataaggtt
     4861 aattgtcccg tttgcgcccg gtggcggcac cgacattacc gcgcgcgccg tcgcgcagaa
     4921 gctctccgag agctggggac aggcggtcgt cgctgacaac cgccccggcg ccaacggcac
     4981 gatcggcgtc gacattacgg cgaagtccgc accggatggc taca
//
LOCUS       DBNGWZ010000273         3828 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2927507, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000273 DBNGWZ010000000
VERSION     DBNGWZ010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2927507"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16CX_08080"
     CDS             complement(<1..337)
                     /locus_tag="AC16CX_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3012506.1"
                     /translation="MAQDRKLIVGILLAAGAGSRFGGEKLLHPLEDGVAIAAHAARNL
                     LAATPDVIAVVRRGDFPLYDILEQEGCQVTMFQGAARGMGASLAHGVSESRSADGWIV
                     ALADMPRIAP"
     gene            537..1007
                     /locus_tag="AC16CX_08085"
     CDS             537..1007
                     /locus_tag="AC16CX_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004543357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012507.1"
                     /translation="MDQKFSFVVDGKSTTLDADPAMPLLYALHSELGLNNPKFGCGKA
                     QCGACTVHLDGEPVRSCVLPVSAAAGKRITTLTGLGTPEKPHPLQQAYIAEQVPQCGY
                     CLSGWIMTAAAFLKNNPKPTDSEIRQAMAGVKCRCGTHMAIMRAIKRASQVMSA"
     gene            1018..3297
                     /locus_tag="AC16CX_08090"
     CDS             1018..3297
                     /locus_tag="AC16CX_08090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012508.1"
                     /translation="MNPITRRDFLKTSGSLVVSVSLPGAIATAMSQGMSAGATLGAKP
                     PLTPDELDSWIAILPDDSVTAFFGKMDMGQGVDVAIAQIVAEELDVAFEKVSVVMGDT
                     ATSCNQGGASGSTGIQHGGVTLRLAAAEARRILVEMASKRIGFPVDQLTVQDGVVMAP
                     GYPLTKVSYGELIGGRHFHHKVGWNKRYGNPLALKVEARPKDPSQYKIVGKSYPQKVV
                     ADKVFGRAEYITDLKVEGMLHARVIRPPNAGCTPVSVDEGSIRNIPAARVVREKDFLA
                     VVAEREWDAVRAAEALKVTWSAQCTPFPPMEKFHQYIREAKPNGKGVPVNKGDVEAAL
                     KGANRVVQAEYEWPLQSHASMAPACAIADVKDGEARLWTGSQKPHFGRDGCAKIAGLP
                     LDKVRATWVMGPGSFGRNDAGDAAHDATLLSKLTGRPVRVQYMRHEGTGWDPKGPAAV
                     FRGRAGLDASGNVVAYEFYGKGFSRQEMATNESDPKDTLAGHLIGYTPKPNIIFQSPA
                     EVYGFENKRCWWETIPPMLDRASPLRVSHFRDPLGPELHFASESFIDEVAHAAGVDPV
                     AFRLRYLSHDPRHAAVVKAVAEKARWQPGPNRNRGKGDVMVGRGISYTERNGTIVAII
                     AEVEVDRRSGRVWAKKFTVAHDCGQIINPGGLKLTIEGNVVMALSRTLFEEVRFTENN
                     VASVDWVTYPILELADAPEAIEIVLINRPEMAPQGAGEPATRTVPAAVANAIFDATGV
                     RVRRVPITPERMKAALASA"
     gene            3435..>3828
                     /locus_tag="AC16CX_08095"
     CDS             3435..>3828
                     /locus_tag="AC16CX_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3012509.1"
                     /translation="MPKFCANLTMLWNELDFLDRFAAAAKAGFRGVEYLFPYDYDKNR
                     VAEQLKKHNLTQVLHNLPAGDWAKGERGIGCHPGRAREFQEGVAKAIDFATALGCKQV
                     NCLAGIRTRHVDPTEAREVFIKNLQFAAP"
BASE COUNT          723 a         1264 c         1215 g          626 t
ORIGIN      
        1 ccggcgcaat gcgcggcatg tccgcgagcg cgactatcca gccgtccgcg cttcgtgatt
       61 cgcttacgcc atgcgccagg ctcgccccca tcccgcgcgc cgcgccctgg aacatcgtga
      121 cctggcagcc ctcctgctcc aatatatcgt agagcggaaa atcaccccgg cgcaccaccg
      181 cgatgacatc gggcgtcgca gcgagcaggt tgcgcgccgc gtgtgccgca atcgccaccc
      241 cgtcctctag cgggtgcagc agcttctcgc caccgaagcg ggagccggct ccagcagcca
      301 gtagtatgcc aacgatgagc ttgcggtctt gtgccatttc ccagccgatt atagcggtgc
      361 aattggcaca atcctcaaag aatccgcgga gttccgcaac aatttgaagc ggcggcttac
      421 ccgtgacacc gcaatcggac cgctttctcc gacatgcccg ggctgccgtt ccacgaaacg
      481 tttgacactc ccattgcccc ggcgctagtg tttaaggcag aaggaggaga gccatgatgg
      541 atcagaaatt cagtttcgtc gtcgacggaa aatccaccac gctcgacgcc gatcccgcga
      601 tgcccctgtt gtacgcgctc cactcggagc tgggcctcaa caatcccaag ttcggttgcg
      661 gcaaggcgca gtgtggcgcc tgcaccgtgc atcttgacgg cgaaccggtg cgctcctgcg
      721 tgctgccggt cagcgctgcc gccggcaaac gcatcaccac gctcaccgga ctcggcacgc
      781 ccgagaaacc ccatccgctg cagcaggcct acattgccga acaggtaccg caatgcggtt
      841 actgcttgtc gggctggatc atgaccgccg cggcgttcct caagaacaat cccaagccga
      901 cggactccga gatccggcag gccatggcgg gtgtcaagtg ccgctgcggc acgcacatgg
      961 cgatcatgcg cgcgatcaag cgcgcttccc aggtgatgag cgcgtgagga ggcaatcatg
     1021 aacccgatta cccgtcgtga tttcctgaag acctccggct cgctcgtcgt cagcgtttcc
     1081 ctgcccggcg caatcgccac ggcgatgagc cagggcatgt cggccggcgc caccctcggg
     1141 gccaagccgc cgctcacgcc cgacgagctc gattcctgga tcgcgatcct gccggacgac
     1201 agcgtcaccg ccttcttcgg caagatggac atgggccagg gcgtggacgt ggcgatcgcg
     1261 cagattgtcg ctgaggaact cgacgtcgcg ttcgagaagg tcagcgtagt catgggtgat
     1321 acggcgactt cctgcaacca gggcggcgcc tccggcagca ccggcatcca gcacggaggc
     1381 gtgacgctgc gcctcgccgc agcagaagcg cgacgcatcc tggtcgagat ggcttccaag
     1441 cgaatcggct tcccggtcga ccaacttacc gtccaggatg gagtagtgat ggctccgggc
     1501 tatccgctga cgaaggtaag ctacggtgaa ttgatcggcg gccggcattt ccatcacaaa
     1561 gtcggatgga acaagcgcta cggcaatccg ctcgccctga aggtggaggc caggcccaag
     1621 gatccctcgc agtacaagat cgtcggcaag tcgtaccccc agaaggtcgt cgccgacaaa
     1681 gtgttcggtc gcgcggagta catcaccgac ctcaaggtgg agggcatgct gcacgcgcgc
     1741 gtgatccgtc cgcccaacgc cggctgcact ccggtttcgg tggacgaggg ctcgatccgc
     1801 aacatccccg ctgcgcgggt ggtgcgcgag aaggattttc tggcggtcgt ggcggagcgc
     1861 gaatgggatg ccgtgcgcgc ggcggaagcc ctcaaggtca cgtggtcggc gcaatgcacg
     1921 ccgttcccgc cgatggaaaa gttccaccag tacatccgcg aggcgaaacc gaacggcaag
     1981 ggcgtccccg tcaacaaggg tgatgtcgaa gcggcgttga agggcgcgaa ccgtgtggtg
     2041 caggcggagt acgaatggcc gctgcagtcg cacgccagca tggccccggc gtgcgccatc
     2101 gccgacgtga aggacgggga agcgcggttg tggactggtt ctcagaagcc gcacttcggt
     2161 cgcgacggat gcgccaagat cgcagggctt ccgctcgaca aagtgcgcgc gacctgggtc
     2221 atgggaccag gctcgttcgg gcgcaacgac gccggcgacg cggcgcacga cgcaacgttg
     2281 ctctccaagc tcaccggcag gccggtgcgc gtgcagtaca tgcggcacga gggcacgggc
     2341 tgggacccca agggcccagc ggcggtgttc cgcggcaggg ccggcctcga tgcttccggc
     2401 aacgtggtcg cctacgagtt ctatggcaag ggattttcgc gccaggaaat ggcgaccaac
     2461 gagtccgacc ccaaggacac gctggccggc catctcatcg gctatacgcc caagccgaac
     2521 atcattttcc agagccctgc agaagtgtac ggcttcgaga acaagcgctg ctggtgggaa
     2581 acgattccac cgatgctcga ccgcgcgtcg ccgctgcgcg tcagccactt ccgcgacccg
     2641 ctcggacccg aactgcactt tgccagcgaa tcgttcatcg acgaggtcgc gcacgcggcg
     2701 ggagtggatc ctgttgcgtt ccggttgcgc tacctctctc acgacccgcg tcacgcagcg
     2761 gtcgtgaagg cggtcgccga aaaggcgcgc tggcagcctg gtcccaatcg caaccgcggc
     2821 aaaggcgacg taatggtggg tcgcggcatt tcctacacgg agcgcaacgg gacgatcgtc
     2881 gccatcatcg ccgaggtcga ggtggaccgc cgcagcgggc gggtgtgggc caagaagttc
     2941 acggtcgccc acgactgcgg ccagatcatc aacccgggcg ggttgaagct cacgatcgag
     3001 ggcaacgtcg taatggccct ctcacgcacg ttgttcgagg aagtgcggtt caccgagaac
     3061 aacgttgcga gcgtggattg ggtgacctac cccatcctcg agctggccga cgcgcccgag
     3121 gcgatcgaaa tcgtgctgat caaccggccg gaaatggcgc cgcagggcgc cggtgagccg
     3181 gcgacgcgta cggtgccggc ggctgtcgcg aacgcgatct tcgatgcaac cggcgtgcgc
     3241 gtccgccgcg tgccgattac gccggagcga atgaaggccg ccctcgccag cgcctgactt
     3301 cacgacaggc ggttctggcg gagagccgcg ctatactgcg gctctccgtt tttgtgcccg
     3361 cccccgtcac tcgtcactcg tcactcgtca ctcgtcactc gtcactcgtc actcgtcact
     3421 aaattagagc ccgcatgccg aaattctgcg ccaacctcac gatgctctgg aacgagctcg
     3481 acttcctgga ccgcttcgcc gcagcagcca aggcgggttt ccggggcgtc gagtacctgt
     3541 ttccttacga ctacgacaag aaccgcgtcg cggagcagct gaaaaaacac aatctgacgc
     3601 aggtgctgca caacctgccg gccggcgact gggcgaaggg cgagcgcggc atcggctgcc
     3661 atcccggccg ggcgagggaa ttccaggaag gcgtcgcgaa ggcaatcgac ttcgccaccg
     3721 cgcttggctg caaacaggtg aactgcctcg ccggtatacg gacgcggcac gtggacccca
     3781 ccgaggcacg cgaggtcttc atcaagaacc tgcaattcgc tgcgccac
//
LOCUS       DBNGWZ010000274         1943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1792718, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000274 DBNGWZ010000000
VERSION     DBNGWZ010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1792718"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="AC16CX_08100"
     CDS             complement(<1..1259)
                     /locus_tag="AC16CX_08100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R family protein"
                     /protein_id="HIR3012510.1"
                     /translation="MNQRRSRSVRARQFGRSRDPHFEREAGRYRHPVPSREFILETLT
                     GQGVPVAEHDLERLLRVTPQQHEAFTRRLAAMERDGEIMRNRRNAICVVEKLDLVRGR
                     VQGHPDGFGFLIRDDGGSDIFLAPKEMRQVLHGDRVVARITGADRRGRPEGKIVEVLE
                     RGQQRVVGRLLREHGILFVAAEDKRVNQEFLVPPDAAAGAKPGQVVMAEVVAQPSKHA
                     QPVARVVEVLGNYADPGMEIEIALRKHALPYAFSQAAGDLCARLPDVVTAEDRHGRVD
                     LSKLPLVTIDGETAKDFDDAVYCEPASNHAGGKGRGGWRLIVAIADVSHYVKHDDALD
                     REARGRGNSVYFPRRVIPMLPEKLSNDLCSLNPKVPRLAVVCDMRIEADGEIASYKFY
                     PAVFRSHARLTYTEVADMLKDEHGEAAR"
     gene            1502..1588
                     /locus_tag="AC16CX_08105"
     tRNA            1502..1588
                     /locus_tag="AC16CX_08105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1536..1538,aa:Leu,seq:cag)
     gene            complement(1635..>1943)
                     /locus_tag="AC16CX_08110"
     CDS             complement(1635..>1943)
                     /locus_tag="AC16CX_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3012511.1"
                     /translation="VIKLFSTLSNVPVAQVLGRQLLRAGTSVGSQYREAMRAKSLADF
                     ISKIEGSLQEVEETGYWLDLLVKSETVPASQIMPIAKEADELTAMLVASVRTAKRRRS
                     "
BASE COUNT          350 a          638 c          584 g          371 t
ORIGIN      
        1 cgcgccgcct cgccgtgctc gtccttcagc atgtccgcga cctcggtata agtgagccgt
       61 gcatgcgagc ggaacacagc cgggtagaac ttgtaggacg caatctcacc gtccgcctcg
      121 atgcgcatgt cgcagactac ggcgaggcgc ggcactttcg gattgagcga gcacaggtcg
      181 ttcgagagct tctccggcag catcggtatg acccggcgcg gaaaataaac cgagttgccc
      241 cgcccgcgcg cctcccggtc gagcgcgtca tcgtgcttca cgtaatgact gacgtcggcg
      301 atcgccacga tcagtcgcca accccctctg ccctttcctc cggcgtgatt ggatgccggc
      361 tcgcagtaca ccgcgtcgtc gaaatccttc gcggtctcgc cgtctatcgt caccaacggc
      421 agcttcgaaa gatcgacacg cccgtgccgg tcctccgcgg tgaccacgtc cggcagccgg
      481 gcgcacaagt ccccggccgc ctgcgaaaac gcgtagggca gcgcgtgctt gcgcagtgca
      541 atctcgatct ccatgccggg gtcggcgtag ttgccgagca cttcgaccac gcgcgccacc
      601 ggctgcgcgt gcttcgaggg ctgcgcgacg acctccgcca tgacgacctg cccgggcttc
      661 gcgccggccg cggcgtccgg gggtaccaga aactcctggt tgacgcgctt gtcctcggcc
      721 gcgacgaaaa gaattccgtg ctcgcgcagc agccggccga cgacgcgctg ctggccgcgc
      781 tccagcactt ccacgatctt gccttccggc cggccgcgac ggtcggcgcc cgtgatgcgc
      841 gccaccacgc ggtccccgtg cagcacctgg cgcatctctt tcggcgcgag aaagatatcg
      901 gacccgccgt cgtcccggat aaggaagccg aaaccgtcgg gatgtccctg gacgcgcccg
      961 cggaccaagt cgagtttctc caccacgcag atcgcgttgc ggcgattgcg catgatctcg
     1021 ccgtcgcgct ccatggccgc gaggcgccgc gtgaacgcct cgtgttgttg cggcgtgacc
     1081 cggagcaagc gctcgagatc gtgctcggct acgggcacgc cttgcccggt cagcgtttcc
     1141 aggatgaatt cccggctcgg caccggatgc cggtagcggc ccgcttcgcg ctcgaagtga
     1201 ggatcgcggc tgcggccaaa ctggcgcgcg cgaacggaac ggcttctgcg ttgattcatt
     1261 tgatcaaatc ggaacgttcg gctagaatcg cgccccgagg agtttgtcag agtttaggcc
     1321 gacaaactcc ttaggcaaaa ccgcctgctg aaaaaccacg aaacaggcac ctggaaacac
     1381 aagctccgat cttgtcgtcc gtcgtaggcg agccgggcat gtttcgctgg gtttcatgtg
     1441 aaatctttag gcggttttgc ttaagcagcc tggtgagacg ctaagtccaa caggctgcta
     1501 cgcccaggtg gcggaattgg cagacgcacc aggttcaggt cctggcgcca gcaatggtgt
     1561 ggaggttcga gtcctctcct gggcaccaac actccaatga tgagtgatga gtgcagagcg
     1621 cagaatgagc aagctcagga ccttctgcgt ttggctgttc gcacgctcgc cacaagcatt
     1681 gccgtgagtt catccgcttc tttcgcgata ggcatgatct gactcgccgg tacggtttcg
     1741 gatttcacca gcaggtccaa ccagtagccg gtctcttcca cttcctgcag cgatccttca
     1801 atcttgctga tgaaatcagc cagggacttg gcgcgcatcg cctcgcgata ttggcttcca
     1861 accgacgtcc ctgcccgcaa tagttggcgt ccaagcactt gagcaaccgg cacatttgaa
     1921 agggttgaga acaacttgat gac
//
LOCUS       DBNGWZ010000275         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_534805, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000275 DBNGWZ010000000
VERSION     DBNGWZ010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_534805"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="AC16CX_08115"
     CDS             complement(<1..1240)
                     /locus_tag="AC16CX_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR3012512.1"
                     /translation="MNDPFDSLQAQLARLEAGEPLETVCAALPEDEAGLLQTAATLRA
                     VPAPERSLPIVSAQRAELLRAARSFKDLNTLPKTDEVRRAGQPARRVWPVALAGGLAA
                     MFVCAFIVAAFAGTFWLWQSSRQPGVARPVSPAPTAVVAFEAPDPQHTVLARASGLVE
                     VQSSGGAWAAAGAGQTLAAGQRVRTGALSSVTLAFYDGSLARLGPNAEVSVDSLDAQK
                     TGARVILLTQWSGDSQHDVAHSDDAASRYEVATPSGVGSAKGTSFRVSVTLLLVRFDV
                     DEGAVAVTNLNVTVIVVAGQTTIIPPGEAPQEPVFHISGEGEVTATGDTWRIGGQTFL
                     TDASTVIVGDPQLGDWVAVEGRLLPDGTRFADRITLLHHSTENQFSFTGKVEVISDTQ
                     WTIAGRVVRVDDLTKIDEEIA"
     gene            complement(1237..1800)
                     /locus_tag="AC16CX_08120"
     CDS             complement(1237..1800)
                     /locus_tag="AC16CX_08120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3012513.1"
                     /translation="MTDQAGVSRPDLVTLAQGGDVQAIGALYDQHHQALFRYIWSRLG
                     ERQTAEELTGDVFMRMLAALPRYRPSVVPFRAWLFRIAHNLLVDHYRKEGGRVSVPLH
                     SAEGVSAEGDPAFLVEQKLTLERVHQALAVVDEPQRSVVALRFLSGLSLEETAAVLDK
                     SESAVKALQHRGLLVLRRALANEEVIS"
     gene            2091..>3260
                     /locus_tag="AC16CX_08125"
     CDS             2091..>3260
                     /locus_tag="AC16CX_08125"
                     /inference="COORDINATES: protein motif:HMM:NF042569.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HIR3012514.1"
                     /translation="MGEITPSAVSLALARRQLAYSGADTIHNAFAAYQREFKVITRRA
                     KARFEGRDWHGAHDDAVERLELYPRIIQQTVAAIRAALGDALHDRPMWMQMKRAYADR
                     IAGRANIEIAETFFNSVSRRIFTTVGVDPNIEFVDSDFEIPSLEADGPIYTAFPWQGT
                     TEALVAAVLRSYAFRSPYHDLERDARLVAERIDAHLEQHRDTRLPDAIEMLKPVFYRG
                     KGAYLVGRLRRGDALTPLALAMLHTETGLHVDAVLLTATEVSIVFSFTRSYFRVEADC
                     PRDLIAFLKSILPQKRIAELYISIGYNKHGKTELYRDLLDHLRRTDERFEPTYGDKGM
                     VMAVFTLHSLNIVFKIIKDTFDYPKATTRQEVMQKYQLVFTHDRAGRLVDAQEFEH"
BASE COUNT          580 a         1111 c         1024 g          545 t
ORIGIN      
        1 ctgcgatctc ctcgtcaatc ttcgtcaaat cgtctacgcg cacgacgcgc ccggcgatcg
       61 tccattgcgt atcgctgatg acctcgacct tccccgtgaa ggagaattga ttctcggtcg
      121 agtgatgtag gagcgtgatc cggtcggcga agcgcgtccc gtccggcagg aggcgcccct
      181 cgacggccac ccagtcgccc aactgcgggt cgccgacgat gacggtgctc gcgtccgtga
      241 gaaaagtctg cccgccgatg cgccaggtgt cgccggtcgc ggtgacctca ccctcgccgc
      301 tgatgtggaa gaccggctcc tgcggagcct cgcccggcgg gatgatggtc gtctgcccgg
      361 cgacgacgat gacggtgacg ttcaagttgg tgacggccac cgcgccctcg tccacgtcga
      421 agcggacgag cagcagcgtg accgacacgc ggaacgacgt gcctttggcc gagccgaccc
      481 cggacggcgt ggcgacttcg tagcgcgagg cggcgtcgtc cgagtgggcc acgtcgtgtt
      541 ggctgtcgcc gctccattgc gtgagcaaga tcacgcgcgc gccggttttc tgggcgtcga
      601 ggctgtccac cgagacctcg gcgttcggcc ccaggcgcgc caggctaccg tcgtaaaaag
      661 ccagcgtcac actggacagc gcgcccgtgc ggactctctg cccggcggcg agcgtctgcc
      721 cggcgccggc ggcggcccac gcgccgccgc tcgattgaac ctcgacgagg ccgctcgccc
      781 tggcgagaac cgtgtgttgc gggtcgggcg cttcgaacgc gacgacggcg gtgggcgcgg
      841 gcgaaacggg ccgcgccaca cccggctgtc tcgacgattg ccacagccag aacgtgcccg
      901 cgaacgcagc cacaatgaaa gcgcacacga acatcgccgc caagccgccg gccagcgcga
      961 ccggccagac ccggcgcgcg ggctgccccg cacgccggac ctcatccgtc ttcggcaggg
     1021 tgttgaggtc tttgaaagac ctggcggcgc ggagcagttc cgcccgctgc gccgagacta
     1081 tcggcagcga gcgctcgggc gcgggcacgg cccggagcgt ggcggccgtc tgcaacagtc
     1141 cggcctcatc ttcgggcagg gctgcgcaca cggtttcgag cggttcgccc gcttccagcc
     1201 gggccaattg cgcttgaagt gaatcaaagg gatcgttcat gagattacct cttcgttagc
     1261 taaagcgcgg cgcagcacga ggaggccgcg atgctgcagc gctttcactg cgctttctga
     1321 cttgtcgagc actgccgccg tttcctccag cgacaggccg ctcaaaaacc tcaacgcgac
     1381 cacgctgcgc tggggttcgt ccacgacggc cagcgcctga tgcacgcgct caagggtgag
     1441 tttctgctcg accaggaagg ccgggtcgcc ctcggcgctc accccttccg ccgagtgcag
     1501 cgggaccgaa acccgcccgc cttccttccg gtaatgatct acgagcaggt tgtgcgcgat
     1561 gcggaacaac cacgcgcgga aaggcacgac ggacgggcga taacgcggca gcgccgccag
     1621 catccgcatg aagacgtcgc cggtcagctc ctcggctgtc tgccgctcgc ccaggcgcga
     1681 ccagatatag cgaaacagcg cctgatgatg ctggtcgtac agcgcgccga tggcttgcac
     1741 gtcgcctccc tgcgcaagcg tcacgaggtc cgggcggctc acgcccgcct ggtcagtcat
     1801 ggcgaagtcc tgctgggtaa gtgagagctc tctcatcacg aagcataacg aagattacct
     1861 ccagaggatt cggatttgcc cgtcggtcac gctttgcgcg acgcgcgata gcggtacggg
     1921 ggtcaccctc caagcataac tttggctcac aggcgtgaca atagccctat aagggtataa
     1981 acgcaggaag agaaaaaggg atgcgcgccg cgtgttaggg tttggtttta tttggtgcta
     2041 aaatctgcgg ccacgtccgg gagcgccgcc accccaggcc tggtccacca atgggcgaaa
     2101 ttaccccgtc cgccgtctcg ctcgccctcg cccgccgcca actggcttac tcgggcgcgg
     2161 atacgatcca caacgctttc gccgcttacc agcgcgaatt caaggttatc acacgccggg
     2221 cgaaagcgcg ctttgaaggg cgggactggc acggcgcgca cgacgacgcg gtggagcggc
     2281 tggagctgta cccgcgcatc atccagcaga ccgtcgccgc gatccgcgcc gcgctcggcg
     2341 atgccctcca cgaccggccc atgtggatgc agatgaaacg cgcgtatgcc gaccggatcg
     2401 cgggccgcgc caacatcgaa atcgccgaga cgttcttcaa ctccgtctcg cgccggattt
     2461 tcaccaccgt gggcgttgac ccgaacatcg agttcgtgga cagcgacttt gaaatcccgt
     2521 cgctcgaagc ggacggtcct atttacacgg ctttcccctg gcaggggacg accgaggcgc
     2581 tggtggccgc ggtcctgcgg tcgtacgctt tccggtcgcc ctatcatgat cttgagcgcg
     2641 atgcgcgcct ggtcgccgaa cgaattgacg cccacctcga gcagcacagg gacactcgcc
     2701 tgcccgacgc cattgaaatg ctcaaaccgg tcttctaccg cggcaagggc gcgtacctgg
     2761 tgggccgcct ccgccgcggg gacgcgttga ccccgctcgc gctcgccatg ctgcacaccg
     2821 agacgggcct ccacgtggac gccgtgctgc tcacggccac cgaggtgagc atcgtcttca
     2881 gcttcacccg ctcgtatttc cgcgtggagg cggactgccc gcgcgacctg atcgcttttc
     2941 tgaaatccat cctgccccag aaacgcatcg ccgagctgta tatctcgatc ggctacaaca
     3001 agcacggcaa gaccgagctt taccgcgatc tgctcgatca cctccgccgg accgacgaac
     3061 gtttcgagcc gacctacggg gacaagggca tggtcatggc ggtgttcacc ctccactcgc
     3121 tgaacatcgt cttcaaaatc atcaaagaca catttgacta tcccaaagcg accacccggc
     3181 aggaagtgat gcagaaatat caactggtgt tcacgcacga ccgcgccggg cggctggtgg
     3241 acgcgcagga gttcgagcac
//
LOCUS       DBNGWZ010000276         5836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1037748, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000276 DBNGWZ010000000
VERSION     DBNGWZ010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1037748"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="AC16CX_08130"
     CDS             <1..873
                     /locus_tag="AC16CX_08130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014066.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HIR3012515.1"
                     /translation="EAAECLGVTAMPGWVARLFSKERNLRMVWEVEKRGRRSMLVGTA
                     HFFPYRFETALRGCISSADTVLLEGPLDEASRRKVVAAGSVRRGASLYDALDAQTIRR
                     INDALAVPSPSSGESRLYWPLLRGEAQDALGEEIRRLEPWMAFFHIWTEYRRRDGWTY
                     SVDLDAARIAAGMNKDVRYLETIEEQIETLGRIPLHRIVSFLKSEDWVTYRRDYVRHY
                     LAGDLAALMAMAQAFPTFCKPVIEERDPVLLERMTPFLERGNAIVFVGILHCPRIIAL
                     LRAHGYAMTGPAAP"
     gene            complement(896..1120)
                     /locus_tag="AC16CX_08135"
     CDS             complement(896..1120)
                     /locus_tag="AC16CX_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR3012516.1"
                     /translation="MARLVKRTATGPYKVEVGSETKFICGCGLSRNQPFCDGTHKTTA
                     RTEEAGKLYWYDAQRNRHECADQYPEIASA"
     gene            complement(1205..2713)
                     /locus_tag="AC16CX_08140"
     CDS             complement(1205..2713)
                     /locus_tag="AC16CX_08140"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR3012517.1"
                     /translation="MLSVLSYGIGTILGFVLIGALIVLIIHDITQKKHTVLRNYPVIG
                     RMRFFFEDLGEYFRQYFFAGDRSEMPFNRATRGYVYRLAKGEGSIIGFGSTNDLREPG
                     SIIFVNHPFPVLEEDRLPTPSLVIGEGYCRLPFEGRSIVNISGMSFGAISAPAVRSLS
                     RGAREAGCWMGTGEGGLAPHHLEGGCDVIMQMGTAKYGMRSLDGSFSPEKVKEIAHHV
                     KAFEIKLSQGAKPGKGGVLPGGKVTDEIARIRGIPAWKDSISPNRHYDIANMNQLLDK
                     VAYLRDLTGRPVGVKTAVGGWEFINDLCDTVLRRGREYAPDFLHIDGGEGGSGAAPQT
                     LMDHMALPIAEALPRVVDSLLQSDLKNRIRVVAAGKLVTSARAAWALCVGADFVVTAR
                     GFMFALGCIQALRCHTNTCPTGVTTHNARLQRGLVVEEKYLRVASYARNMNKEIDMIA
                     HSCGCRHARELKREHVRIVQTANQSIALNMLYPYPTVGSRVASKQATASAAA"
     gene            2776..3609
                     /gene="nadC"
                     /locus_tag="AC16CX_08145"
     CDS             2776..3609
                     /gene="nadC"
                     /locus_tag="AC16CX_08145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3012518.1"
                     /translation="MDLASVIERDVTAALAEDVGSGDATAPLLPAQKTARAVVSCRQQ
                     AVVCGQAWFEACFRRLDAGATIKWLVAEGAQVVPGARVCEVRGQARALLTAERAALNF
                     LQTLSAVATVTRQHVEAIAGTRTKIVDTRKTLPGLRYAQKYAVRTGGGANHRMGLYDA
                     MLIKENHIAAAGGIRQAMAEAKKHADLGIWVQIEVENLDQLREALDAGATMVLLDNMS
                     LETMREAVRIAGDRAELEASGGITLEKVRAIAETGVHRISVGALTKDVKAVDFSMRFE
                     T"
     gene            complement(4064..5071)
                     /locus_tag="AC16CX_08150"
     CDS             complement(4064..5071)
                     /locus_tag="AC16CX_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012519.1"
                     /translation="MEFPSRLVLDAPAAVLSWIGRQGTRAVAALIVIGIALPSIGALL
                     KPFVTEAVFVLLCIAFLRVDAAAFRGYVGRPMIVIAATAWTSLAIPTMFGAGYLAFGL
                     RDQSPDLFLALVLQAIASPLMAAPAFAALMGLDATLVLIAMVMSTALLPFTAPLFAYA
                     FIGPALTLSPLALGLKLFAILAGSALVGFIVRRIAGFAAIERQKERIDGFNILVLFVF
                     IAAIMEGVAARFLVTPMITIGLVALAFVVFFAVLCLTIPLFAFAGRGRALTLGFMASQ
                     RNVGLMLAATAGALPDLTWLYFAFCYFPIYLSPLLLQSLARRIIARAQTAPAPPRGPT
                     T"
     gene            complement(5140..5652)
                     /locus_tag="AC16CX_08155"
     CDS             complement(5140..5652)
                     /locus_tag="AC16CX_08155"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3012520.1"
                     /translation="MDADELRAVQGPLKERYRQAPGAALITLRAHGRLGEGVSCKIET
                     GKALVTAGLHPATGGTGLSACSGDMLLEALVACAGVTLNAVATSLGIKLRDARLEAEG
                     DLDFRGTLGVSKEVPVGFQSIRLKLALDTDASEEQLATLLRLTERYCVVYQTLAHPPA
                     VTVARQGAGA"
BASE COUNT         1130 a         1808 c         1872 g         1026 t
ORIGIN      
        1 gaagccgccg agtgcctcgg agtgaccgcc atgcccggct gggtcgcgcg cctgttctcg
       61 aaggagagga acctcaggat ggtctgggag gtggagaaac gcgggcggcg ttcgatgctc
      121 gtcggcaccg cgcacttctt cccctaccgg ttcgagaccg ccctgcgcgg ctgcatcagc
      181 agtgcggaca cggtgctgct tgaaggcccg ctggatgaag cctcgaggcg aaaagtcgtc
      241 gcggcgggat cggtccggcg cggcgcttcg ctctacgacg cgctggacgc gcagacgatt
      301 cgcaggatca atgatgcgct cgccgtcccg tctccctcct ccggcgaaag ccggctctac
      361 tggcccctat tacgcgggga ggcgcaagac gctttgggcg aggagatccg gcggctggag
      421 ccctggatgg ccttctttca catctggacg gaatatcgaa ggcgcgacgg atggacctac
      481 tcggtggatc tcgacgccgc acggatcgcg gccgggatga acaaggacgt gcgctacctc
      541 gaaacgatcg aggaacagat cgagacgctg ggccgtatcc cgctgcatcg catagtgagc
      601 tttctgaaga gcgaggactg ggtgacctac cgtcgggact acgtgcggca ttatctcgcg
      661 ggcgacctcg ccgccctgat ggccatggcg caggcgtttc cgaccttctg caagcccgtc
      721 atcgaggaac gcgaccctgt cctgctcgag cggatgaccc cgtttctcga gcgcgggaac
      781 gcgatcgtgt tcgtcggaat tctccattgc ccccggatca tcgcgctgct gcgcgcgcac
      841 ggctacgcca tgacgggccc tgcggctccc tgagcgcgtc tcgcgccggg aagcgttacg
      901 cgctcgcgat ttcgggatac tggtccgcgc actcgtggcg gttgcgctgc gcgtcatacc
      961 agtacagctt gcccgcctcc tcggtgcgcg cggtcgtctt gtgcgtgccg tcgcagaatg
     1021 gctggttccg cgacaacccg cagccgcaga tgaacttggt ttcgctcccc acctcgactt
     1081 tgtaggggcc ggtggctgtg cgcttgacga gtcttgccat acttcctccg tagctatcag
     1141 ctgtcagctt gaagcaggat ttgtcgcctt ggctgatcgc tgaaagctga cagctgtatg
     1201 cttcctacgc cgcagcgctc gccgtggcct gcttcgacgc gacccgcgag cccaccgtcg
     1261 gatacggata gagcatgttg agcgcgatgc tctgattcgc ggtctgcacg atgcgcacgt
     1321 gctcgcgctt gagctcgcgt gcgtggcggc agccgcagga gtgcgcgatc atgtcgatct
     1381 ccttgttcat gttgcgcgcg tagctcgcca cccgcaggta cttctcctcg accaccagcc
     1441 cgcgctgcag ccgcgcgttg tgggtggtga cgccggtcgg acaggtgttg gtgtggcacc
     1501 gcaacgcctg gatgcacccg agcgcgaaca tgaaaccgcg ggcggtcacc acgaaatccg
     1561 cgcccacgca caacgcccag gcggcgcgcg cggaagtgac gagcttcccg gcggcgacca
     1621 cccggatgcg gttcttgagg tcggactgca gcagcgagtc caccacccgc ggcagcgctt
     1681 ccgcgatggg cagagccatg tgatccatca aggtttgggg tgcagcgccg ctgccgcctt
     1741 ctccgccatc gatatggaga aagtccggcg cgtactccct gccgcgccgc agcaccgtgt
     1801 cgcacaggtc gttgatgaat tcccagccgc cgacggcggt cttcacgccc accggacggc
     1861 cggtgagatc gcgcagatag gcgaccttgt cgagcagctg gttcatgttg gcgatgtcgt
     1921 agtggcggtt ggggctgatc gaatctttcc aggccgggat gccgcggatg cgcgcaattt
     1981 catcagtgac cttgccgccc ggcagcaccc cgcccttgcc cggcttcgcg ccctgcgaga
     2041 gcttgatctc gaacgccttc acgtgatgcg cgatctcctt caccttctcc ggcgaaaagc
     2101 tgccgtcgag gctgcgcatg ccgtacttgg cggtgcccat ttgcatgatg acatcgcagc
     2161 cgccttcgag gtgatggggc gcgagcccgc cttccccggt tcccatccag cagccggcct
     2221 cgcgcgcgcc gcgcgagagc gagcgcaccg ccggcgcgga gatcgcgcca aagctcatgc
     2281 cgctgatgtt gacgatggag cgcccctcga acggcagcct gcagtagcct tcgccgatca
     2341 cgagcgaggg cgtgggtagc cgatcctctt ccagcaccgg gaagggatga ttgacgaaga
     2401 tgatcgagcc cggctcgcgc agatcgttgg tcgagccgaa gccgatgatg ctgccctcgc
     2461 ccttggcgag gcgataaaca tagccccgcg tcgcgcggtt gaacggcatt tcggagcggt
     2521 cccccgcgaa gaagtactgc cggaaatact cgccgaggtc ctcgaagaag aagcgcatcc
     2581 gcccgatcac cgggtagttg cgcagcactg tgtgcttttt ctgggtgatg tcgtggatga
     2641 tcagaacgat cagtgcgccg atcagcacga agcccagtat cgtgccgatg ccatacgata
     2701 gaacgctcag catgcctcca cctcgccccc ttcaaatgat gcgttagaat tgtctcttaa
     2761 ctatagagga aatcggtgga tttggcatct gtcatcgagc gcgatgtgac cgccgccctc
     2821 gccgaggacg tcggcagcgg cgacgccacc gcgccgttgc tgccggcgca aaagaccgcg
     2881 cgggccgtgg tgtcatgccg gcagcaagcg gtggtctgtg gacaggcgtg gttcgaggcg
     2941 tgctttcgca ggttggatgc gggcgccacc atcaagtggc tcgtggccga gggtgcgcag
     3001 gtcgttcccg gcgctcgcgt ttgcgaagta cgcggacagg cgcgcgcgtt gctgaccgcc
     3061 gagcgcgccg ctctcaattt tcttcagacc ttgtccgccg tggcgaccgt gacgcgccaa
     3121 cacgtggaag ccatcgccgg cacaaggacc aaaatcgtgg acacccgcaa gacgctgccc
     3181 ggcctgcgct acgcccagaa atacgcggtg cgcaccggcg gcggcgccaa ccaccgcatg
     3241 gggctctacg acgcgatgct gatcaaggaa aaccacatcg ccgcggcggg cggcatccgg
     3301 caggcgatgg cggaagcgaa aaaacacgct gacctgggaa tctgggtgca gatcgaggtc
     3361 gagaatctcg atcagctgag ggaggcgctc gatgccggcg cgaccatggt cctgctcgac
     3421 aacatgagcc tcgagacgat gcgcgaggcg gtgaggatcg cgggcgaccg tgccgagctg
     3481 gaggcgtcgg gcggcatcac gctggaaaag gtccgcgcga tcgcggagac tggggtgcat
     3541 cgcatctcgg tcggggcgct gacgaaggat gtgaaggctg tcgatttctc gatgcggttt
     3601 gaaacctgaa ccgcctggag attcaaatca atatcgaacc gccaagacgc caagagaagc
     3661 aatgacaata acaaagtcaa aaaccaatcg aaccgcggag atggggagag aaacaataat
     3721 tgaacccgca aaggctgctc aatattggtg atggtgatga gtcacccgca cgccgcgcgg
     3781 gccgagtcac gagtcacgat tcgcgagtca cgagatcaag gacgctaaaa caacgctaag
     3841 ctagggtggc atgtaggaaa cgccattggt tattttccgc ttttattctc gtttgccgca
     3901 aaaaccgtgg tctgaccccc attactgact cagaatataa aaaacgcaat tgagactttt
     3961 acaaatcgaa ttcacctcaa gcccggcccc tatgattaat ttattcgagc ttggcccctt
     4021 tttcactttg tgttgattgg gcggtcaggc cttagtcgac ttgtcatgtg gtcggccctc
     4081 gtggcggtgc gggagcggtc tgcgctcggg cgattatccg gcgcgccagc gattggagca
     4141 gcagcggcga cagatagatg ggaaagtagc agaaggcgaa atacagccag gtgaggtcgg
     4201 gcaaggcccc ggcggtcgcc gccagcatca gccccacgtt gcgttgcgag gccatgaatc
     4261 cgagcgtaag ggcgcgtccc cggcccgcga atgcgaagag aggaatggtc aggcaaagca
     4321 ctgcgaagaa caccacgaag gcaagcgcaa cgaggccaat cgtgatcatg ggcgtgacga
     4381 gaaagcgagc ggcgacgcct tccatgatgg ccgctatgaa gacgaacagg acgagaatgt
     4441 tgaagccgtc gatcctctcc ttctgccgct cgatggcagc gaagccggcg atccggcgca
     4501 cgatgaaacc gacaagggcg gagcccgcga ggatcgcaaa caacttgagc ccgagcgcca
     4561 atggggagag tgtaagcgcg ggtccgatga aggcataggc gaacaaaggc gctgtaaagg
     4621 gtaacagcgc cgtgctcatc accatggcga tgagcacgag cgtggcatcc agccccatca
     4681 acgcagcaaa cgccggagcc gccatcaagg gcgaggcaat ggcttgcagc acgagggcga
     4741 ggaagagatc gggtgattga tctctgagac cgaacgccag atagcccgcg ccaaacatgg
     4801 ttggaatcgc caaggatgtc catgctgtgg ctgcgatcac gatcatgggg cgtcccacgt
     4861 aacccctgaa cgccgcggcg tccacgcgca ggaaggcaat gcacaggagc acaaagaccg
     4921 cctcggtgac gaacggcttg agcagggcgc cgatcgatgg cagggcgata ccaatgacaa
     4981 tgagcgctgc gactgcgcgg gtgccttgcc ggccgatcca cgacagcacc gctgcgggtg
     5041 cgtcaagaac gagcctgctc gggaactcca tcgttatttc tcattggggg caaaccgcgg
     5101 tttcgggtac agccaactct cataaaccgt ggcccctttt tacgccccag ctccctgccg
     5161 ggcgacagtc actgccggag ggtgagccag ggtctggtag accacgcagt agcgctcggt
     5221 caggcgcagc aaagtcgcga gctgctcttc cgacgcgtcg gtgtcgagcg cgagcttcag
     5281 ccggatgctt tgaaatccca ccggcacttc ctttgacacg ccgagcgtgc ctcgaaagtc
     5341 gagatcgcct tccgcctcga ggcgggcatc gcgaagcttg ataccgagcg acgttgccac
     5401 ggcgttcagg gtaacacccg cacaagccac cagggcttcg agaagcatgt ccccggaaca
     5461 ggcactgagg ccggttcctc ccgtcgccgg atgcagcccg gcggtgacca gcgccttacc
     5521 cgtttcgatc ttgcagctga cgccctcgcc gagccggcca tgcgcccgga gggtgatcag
     5581 ggctgcgccg ggcgcctggc ggtaacgttc cttcaacggc ccctgcacgg cccgtagctc
     5641 gtcggcgtcc atggcgctgt ctcctttgcc cgcaagtatg caatcaaaag ggccaggctg
     5701 aacttacaag aaatcgagcc tggcccctcg tactactctg cgggcaaaaa atctgtcgtg
     5761 ggaaagtagg gggatgcaca ccttcctgag cgcgtcccct ctttctagaa cgtgatcaca
     5821 cggtcggcat ccaccg
//
LOCUS       DBNGWZ010000277         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_658362, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000277 DBNGWZ010000000
VERSION     DBNGWZ010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_658362"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..>1847
                     /locus_tag="AC16CX_08160"
     CDS             34..>1847
                     /locus_tag="AC16CX_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR3012521.1"
                     /translation="MEAEEGATNRLTGLLGRFVVVVAVIMSFFHLYAAYAIVPTQQLR
                     MIHVGFVLFLTYLLFPVSRGYRHRVMWWDWIAAAVSLVVIGYGIAGGDDFTDRSTIPN
                     NVDMVFGVILTLLVLEAARRGTGWIMPLVCVAFVAYAMLGPYLGEPWTHRGYDISRLV
                     GHMYMTLEGIFGVPVDVSSSLIILFTIYGAFLQFSGAGKFFIDFSFAAMGGKSTGAGR
                     TVVLSSFLLGGPSGSGVATTVTLGTVAYPMLAKAGYGKDAAGGLLAAGGIGAIISPPV
                     LGAAAFLIAEFLKISYLDVILMATIPTCLYYFALFLMVELDARKFGMKEVQFDKTETL
                     WNLTKKFGFHFLSLVAIIFFMLLGFSPVSAVFWATAVAFVTSALHPDCALMSWRQMVI
                     FCVVVVASCLIYTAVKGGFHQDALTWALPAGALALVAAAFIRPELRVWPEKLVKALEV
                     GSVSVLNVAATCAAAGIIVGVVTLTGLGLKFSSIVIGYADSLTQGTMGLLSAIGVSAT
                     QALLHDVKLLYTAIFTSLIVWIVGLAVPVTASYIICAVIAAPALITLGVADFAAHMFI
                     FYYAVLSEVSPPTALSPFAAAAITGGDPYKTTLQSWKYT"
BASE COUNT          247 a          611 c          598 g          391 t
ORIGIN      
        1 gacgagtctg cgctcaagaa agccgaggag tacatcgagg cggaggaggg cgcgaccaac
       61 cgcctgaccg gtctcctcgg ccgcttcgtg gtggtggtgg ccgtcatcat gtcgttcttt
      121 cacctctatg cggcctatgc catcgtgccg acacagcagc tgcgcatgat ccacgtcggc
      181 ttcgtgctgt tcctgaccta cctgctgttc ccggtttccc ggggctaccg tcaccgtgtc
      241 atgtggtggg actggatcgc ggcggcggtt tcgctcgtgg tcatcggcta cggcatcgcc
      301 ggcggcgacg atttcaccga ccgcagcacc atcccgaaca acgtcgatat ggtgttcggc
      361 gtgatcctga cgctgctcgt gctggaggcg gcgcgccgcg gcaccgggtg gatcatgccg
      421 ctcgtgtgcg tggcttttgt ggcgtatgcg atgcttggcc cgtacctggg cgagccgtgg
      481 acacaccgcg gctacgacat ttcgcggctg gtcggccaca tgtacatgac cctggaggga
      541 atcttcggcg tgccggtgga cgtctcctcc tcgctcatca tccttttcac catctacggc
      601 gccttcctgc agttttccgg cgccggcaag ttcttcatcg acttctcgtt cgccgcgatg
      661 gggggcaagt ccaccggcgc cgggcgcacc gtcgtgctct cgtcgttcct gctgggcggc
      721 ccgtcgggca gcggggtcgc caccaccgtg acgctcggca ccgtcgccta tcccatgctc
      781 gccaaggcgg gctacggcaa ggacgcggcg ggcggattgc tggcggcagg cgggattggc
      841 gcgatcatct caccgccggt gctgggtgcg gccgccttcc tcatcgcgga gttcctcaag
      901 atctcatatc tcgacgtgat cctgatggcg accattccga cctgcctcta ttacttcgcg
      961 ctgttcctga tggtcgagct cgacgcgcgc aagttcggga tgaaggaggt gcagttcgac
     1021 aagacggaga ccctctggaa cctcaccaag aaattcggtt tccacttcct gtcgctggtc
     1081 gcgatcatct tcttcatgct gctcggcttc tcgccggtct cggcggtgtt ctgggcgacc
     1141 gcggtcgcgt tcgtcaccag cgcgctgcac cccgactgcg cgctgatgag ctggcggcag
     1201 atggtcattt tctgcgtcgt cgtggtcgcg tcgtgcctga tttacacggc ggtcaaaggc
     1261 gggttccatc aggacgcgct gacctgggcg cttcccgccg gggcgctcgc cctcgttgcc
     1321 gccgccttca tccggcccga actgcgcgtc tggcccgaaa agctcgtgaa ggcgcttgaa
     1381 gtcggttcgg tcagcgtgtt gaacgtcgcg gcgacctgcg cggcggcggg catcatcgtg
     1441 ggcgtggtga cgctgaccgg cctcggcctc aagttcagct cgatcgtgat cggctacgcc
     1501 gactcgctca cccagggcac gatgggcctg ctgtcggcga tcggggtgtc ggccacgcag
     1561 gcgctgctgc acgacgtgaa gctgctctat acggcgatct tcacctccct catcgtgtgg
     1621 atcgtggggc tcgccgtgcc ggtcacggcg tcctacatca tctgcgccgt gatcgccgcg
     1681 ccggcgctga tcacgctcgg cgtcgcggat ttcgcggcgc acatgttcat cttctactac
     1741 gcggtgctgt cggaggtctc gccgcccacc gcgctctcgc cgttcgcggc ggccgcgatc
     1801 accggtggcg acccgtacaa gacgacgctg cagtcgtgga agtacac
//
LOCUS       DBNGWZ010000278         3808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_658456, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000278 DBNGWZ010000000
VERSION     DBNGWZ010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_658456"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16CX_08165"
     CDS             complement(<1..337)
                     /locus_tag="AC16CX_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3012522.1"
                     /translation="MNVLDDFHPAVANWFRNTFAAPTEPQERAWPAIRSGSDVLIAAP
                     TGSGKTLAAFLAAIDALVRLGARRGLGDETRVVYVSPLKALSNDIHKNLEEPLAGIRK
                     ELAALGHPDV"
     gene            complement(457..1023)
                     /locus_tag="AC16CX_08170"
     CDS             complement(457..1023)
                     /locus_tag="AC16CX_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR3012523.1"
                     /translation="MEALIVSTSVVALAEMGDKTQLLSFVLAARLKRKGWIILGILFA
                     TLANHFLAGYAGASLAKLVSPQVLRWIVALSFFAFGAWALRKDKLEENRNLRGAGVFL
                     TTLIAFFLVEMGDKTQLATVALAARYDSLVVVVLGTTLGMMIANVPAVWIGEALAHRV
                     NMKMMRWVAAGLFVLLGVLALLATTPAA"
     gene            complement(1069..1830)
                     /locus_tag="AC16CX_08175"
     CDS             complement(1069..1830)
                     /locus_tag="AC16CX_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3012524.1"
                     /translation="MIRGSSGQEQAVRKSRFFPAHDPRPTTHAIAAAAALLLFAAGSA
                     IGGEAEWKLLHDQAAAHLQRGSLEQAGLFAREALKEAESSLGPEHRATEQSLSTLSLA
                     LRLQGKQDEALPLVQRLVAIRTKQYGPEDPSTAIALHNNAETLIALNRFAEAVVLQAR
                     ALAVFEKKLGASHVNTATALHNLGAILLKQEKYKDAEKYLRRALAAKEKALKPGHLSI
                     AHTLDNLSAALDAQGRQLEAEKYKRRAEAIRRKAG"
     gene            complement(1827..>3808)
                     /gene="dnaE"
                     /locus_tag="AC16CX_08180"
     CDS             complement(1827..>3808)
                     /gene="dnaE"
                     /locus_tag="AC16CX_08180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3012525.1"
                     /translation="IAPGKLTDFCPLYAADGSDSVVSQFDMKDVEAVGLVKFDFLGLT
                     TLTILDWTVKYIQKAEGETADSTDPSSFILHPSSFDLSSIPLDDAATYRLFAAADTTG
                     VFQFESRGMRDLLARAKPDHFEDIVALVALYRPGPMELIPEFVRRKQGGRVDYPDPRV
                     EPILKPTYGIMVYQEQVMQIAQVIGGYSLGAADLLRRAMGKKLPEEMAQHRSVFVAGA
                     AKNGVSARKAHELFDLMEKFAGYGFNRSHAAAYALVAYQTAWFKAHHTAAFIAANMSA
                     VMSDTDKVQIFAEDARAHGIELAAPDINRSNHRFEPVDGKTIQYGLGAIKGTGEAAIN
                     SIVEARRQGPFKDLFDFCHRVDKRLVNRRVVESLIRAGAFDPVNDHRASLLASVGQAL
                     ESAEQASRAAHQVSLFGDLAETAERPALAETPRWGARERLQNEKLALGFYFSDHLFNI
                     YREEVRRFIRTRLADLGAGGNGDYAGRAYWVAGVVIGTRVQTTASGRMGVIQLADDSG
                     RFEIVAFREVFEKFRQKLKEDELLVLEVRLRAARMRAAAGEGETAGPDFGMRIEALNV
                     LDLAEARNRFARGVRLNCNGESSGEKLRDLLAPYRSGHCPVSVVYSNRGASCEIDLGD
                     AWRVSLHDDLIRSLSDWLSPENVKIVYNETVDRGS"
BASE COUNT          651 a         1257 c         1218 g          682 t
ORIGIN      
        1 cgacgtcggg atgaccgagc gcggcgagct ccttgcggat tcccgcgagc ggctcttcga
       61 ggttcttgtg gatgtcgttg gagagcgcct tgagcggcga gacgtacacc acacgggtct
      121 cgtccccaag cccgcgccgc gcgccgagcc gcaccagcgc gtcgatggcg gcgaggaacg
      181 ccgcgagcgt cttgccggag ccggtcggcg ctgcgatcag cacatcactg cccgagcgga
      241 tcgccggcca ggcccgctcc tgcggctcgg tcggtgcggc aaaagtgtta cggaaccagt
      301 tcgcgaccgc tggatggaaa tcgtcaagga cgttcatgcg cgaaacttat ctgaaaaacc
      361 cccgaccgcc aacaaaaccg gaaatcgacg atttaacgcc ggacatcctc cgcccgcgcc
      421 agcgcgtcat ggctgcagcc ctgcttggag gcggaactac gcggccggag tcgtcgcaag
      481 caatgccagg acgcccagga gaacgaacag cccagcagcc acccagcgca tcatcttcat
      541 attgactcga tgggccagcg cttcgccgat ccataccgcc gggacgttcg cgatcatcat
      601 gcccagggtc gttcccagaa cgaccacgac gagtgaatcg tagcgggccg ctagtgccac
      661 ggtggcgagt tgagtcttat cgcccatctc caccagaaag aaggcgatga gcgtcgtcag
      721 gaacacaccg gcgccccgga ggttccggtt ttcctccagc ttgtccttcc tcagcgccca
      781 ggcgccgaag gcgaagaacg aaagggcaac aatccacctc agcacctgag gcgataccag
      841 tttcgcaagc gaggcccccg cgtagcccgc gagaaaatgg ttggcgagcg tggcgaagag
      901 aatgcccagg atgatccacc ccttgcgctt gagccttgcc gccagcacaa atgacagaag
      961 ctgggtttta tcgcccattt cggccagtgc gacgaccgag gttgagacga tcagagcttc
     1021 caagcagttt ctccgcctct gacatcaatg agtgattacg gttttgactc acccggcctt
     1081 gcgccggatc gcttccgccc ggcgcttgta tttctccgcc tcgagctgtc ggccctgcgc
     1141 gtcgagcgcg gcggagagat tgtccagcgt gtgcgcgatg ctgagatggc cgggcttgag
     1201 ggccttctcc ttcgccgcca gcgcgcggcg caggtatttt tccgcgtcct tgtacttttc
     1261 ctgcttgagc aggattgctc ccaggttgtg cagcgcggtg gcggtgttga cgtgactcgc
     1321 ccccagcttc ttttcgaaca ccgcgagggc tctcgcctgc agcaccaccg cctcggcgaa
     1381 ccggttcagc gcgatgaggg tctcggcatt gttgtgcagc gcaatggcag ttgatggatc
     1441 ttccggtccg tactgtttcg tccggatggc caccaggcgc tggaccagcg gcagggcttc
     1501 atcctgcttg ccctgcaaac gcaacgccag ggaaagcgtc gaaagactct gttccgtggc
     1561 ccggtgctcc ggcccgagcg aagattcggc ttccttgagc gcctcgcgag cgaacagccc
     1621 cgcctgttcg agactgccgc gttggaggtg cgcagcggcc tggtcgtgca gcagcttcca
     1681 ctccgcttca ccgccgatcg ctgatccggc ggcgaacagc agcagcgccg cggctgctgc
     1741 aattgcgtga gtcgtgggtc gtgggtcgtg ggccgggaaa aatcgacttt tgcgaacagc
     1801 ttgttcttgt cctgacgatc cacgaatcac gatccacgat ccacggtttc gttgtagacg
     1861 atcttgacgt tctcgggact cagccagtcg gagagcgagc ggatcaggtc gtcgtgcagg
     1921 ctcacccgcc aggcgtcgcc gaggtcgatc tcgcagctcg cgccgcggtt ggaatagacc
     1981 accgacaccg ggcagtgacc gctgcgatag ggcgccagga gatcgcgcag cttttcgccc
     2041 gacgattccc cgttgcagtt gagccgcacg ccgcgcgcga agcggttgcg cgcctcggcg
     2101 aggtcgagca cgttgagcgc ctcgatgcgc atgccgaaat cggggccggc ggtctcgccc
     2161 tcgccggccg ccgcgcgcat gcgcgccgcg cgcagccgca cctccagcac cagcagctcg
     2221 tcctccttga gcttctgccg gaacttctcg aacacttcgc ggaacgccac gatctcgaag
     2281 cgcccggaat cgtccgcgag ctggatgacg cccatgcgcc cgctcgccgt ggtctgcacg
     2341 cgcgtgccga tcaccacgcc cgccacccag taagcgcggc cggcgtaatc gccgttgccc
     2401 cccgcgccga ggtcggcaag acgggtgcgg atgaagcggc ggacttcctc gcgatagatg
     2461 ttgaacaggt gatcgctgaa gtagaagccg agcgccagct tctcgttttg cagccgctcc
     2521 ctggcgcccc agcgcggcgt ctcggcgagc gcgggccgct ccgcggtttc cgcaaggtcg
     2581 ccgaacaggc tcacctggtg ggcggcgcgg ctcgcctgct ccgcgctctc gagcgcctgc
     2641 ccgaccgagg cgagcaggct cgcgcggtgg tcgttcaccg ggtcaaaggc cccggcgcgg
     2701 atgagcgact ccaccacacg gcggttgacg aggcgcttgt ccacgcggtg gcagaagtcg
     2761 aacaggtcct tgaacggtcc ttgcctgcgc gcctcgacga tggagttgat cgcggcctcg
     2821 cccgtgccct tgatcgcacc gaggccgtac tggatggtct tgccgtccac cggctcgaag
     2881 cggtggttcg agcggttgat gtcgggcgcg gcgagctcga tgccatgcgc ccgcgcgtct
     2941 tcggcgaaga tctgtacctt gtccgtgtcg ctcatcaccg cggacatgtt cgctgcgatg
     3001 aaggccgccg tgtggtgcgc cttgaaccat gcggtctggt acgccaccag cgcgtacgcg
     3061 gcggcgtgag atctattgaa accgtaacct gcgaacttct ccatcaggtc gaacagctcg
     3121 tgggccttgc gcgcgctgac gccgtttttc gccgcgcccg ccacgaacac gctccggtgc
     3181 tgggccatct cctcgggcag cttcttgccc atcgcgcggc gcagcagatc ggccgcgccg
     3241 aggctgtagc cgccgattac ctgcgcgatc tgcatcacct gttcctggta caccatgatg
     3301 ccgtaagtgg gcttgagtat cggctccacc cgcggatccg ggtaatccac gcgtccgccc
     3361 tgcttgcggc gcacgaactc cgggatcagc tccatcggtc ccggccggta gagcgcgacc
     3421 agcgcgacga tgtcctcgaa gtggtcgggc ttcgcgcggg cgaggagatc gcgcatgcca
     3481 cgcgattcaa actggaacac cccggtcgtg tcggcggccg cgaataaccg gtaagtcgcc
     3541 gcgtcatcca gaggaatgga ggaaagatca aaggatgaag gatgaaggat gaaggatgaa
     3601 gggtcggtcg aatcggccgt ttcgccttct gccttctgga tgtacttcac cgtccagtcg
     3661 aggatggtga gcgtggtcag cccgaggaaa tcgaacttga ccagcccgac cgcctcgacg
     3721 tccttcatgt cgaactggga caccacgctg tcggagccgt ccgcggcgta gagcgggcag
     3781 aaatcggtga gcttgcccgg cgcaatca
//
LOCUS       DBNGWZ010000279         3104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2423879, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000279 DBNGWZ010000000
VERSION     DBNGWZ010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2423879"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16CX_08185"
     CDS             <1..311
                     /locus_tag="AC16CX_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR3012526.1"
                     /translation="REGRAVATLHPEKRIYRVQETPMTESAIDYGAFRHLYVALAEAV
                     GPTAWIVRVHYKPFVGWIWYGWVLMALGGLLAATDRRYRVAAREPRPAAYAAEAGAAR
                     "
     gene            575..1102
                     /locus_tag="AC16CX_08190"
     CDS             575..1102
                     /locus_tag="AC16CX_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HIR3012527.1"
                     /translation="MTRYLLPLGVFALLVLLLGVGLSLDPREVPSPLIGKPAPAFQLP
                     QLAQPEKSFSPKELQGKVWVLNVWASWCAACREEHPVLTDLARSGIAPVYGLNYKDRR
                     DEALAWLRRFGDPYRASLFDADGRVGIDYGVYGVPETYVIDKQGVIRYKRIGPVTTEI
                     VQKKIAPLIQQLNGG"
     gene            1136..1609
                     /locus_tag="AC16CX_08195"
     CDS             1136..1609
                     /locus_tag="AC16CX_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR3012528.1"
                     /translation="MRSRAIFFILILLFAPWAHAGEAKPAADDPVLEERMMALAAELR
                     CLVCQNQTLADSNAPLAVDLRNQVREQMRQGKSEREIIDYMVARYGDFVLFRPPFKLT
                     TLLLWTGPLLLLVAGLIALFYRLARRRAEPKAELSDEERARAAALLEGDADMERR"
     gene            1606..2820
                     /gene="ccmI"
                     /locus_tag="AC16CX_08200"
     CDS             1606..2820
                     /gene="ccmI"
                     /locus_tag="AC16CX_08200"
                     /inference="COORDINATES: protein motif:HMM:NF048240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HIR3012529.1"
                     /translation="MTAVFWILAALLAAVALAFVLPALLGLRHRAESAAADATNIAVY
                     RDQLKELEADLAAGTLNREQYDEARREIERRMLEDVRVPKTTAHAAKSGREAAIAAAI
                     AVPLVSVLLYLAVGNPKALAPRQDGGGAAHELTAGQIEALVERLAARMRDNPEDAQGW
                     IMLGRSYTVLGRFADATQAYAQAAARLPRDAQVLADYADALAMAQGKRLVGEPERLIA
                     RALALDPDNLKALALAGTVAFEKKDYASAVRHWERMLPLVPGNSEVERSIKASIAEAR
                     SLGGGGQPARSAAPGKGVRGVVKLAPQLAAKAAPTDTVFIFVRAAEGPRAPLAVLRKQ
                     VRDLPAEFALDDTMAMAPGMTLSSFPRVVVGARISKTTNAAPQPGDLQGMSPPVRNDA
                     SGVTVVINTETR"
     gene            complement(2840..>3104)
                     /locus_tag="AC16CX_08205"
     CDS             complement(2840..>3104)
                     /locus_tag="AC16CX_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3012530.1"
                     /translation="VVSATSPQMPNATANGVEGDLREQLRRVEAEILLRAIDDAGGDR
                     RRAAQRLGIGLSSLYRKLTELEQQGVIQGMAPSSSKDLSAPRG"
BASE COUNT          521 a         1035 c         1011 g          537 t
ORIGIN      
        1 cgcgggaggg ccgcgccgtc gcgacgctcc acccggagaa gcgcatctac cgcgtgcagg
       61 aaacgccgat gaccgagtcc gcgatcgact acggcgcttt ccgccacctc tacgtcgcgc
      121 tggcggaggc ggtcggcccg accgcctgga tcgtgcgcgt gcactacaaa ccgttcgtcg
      181 gctggatctg gtacggctgg gtgctgatgg cgctcggcgg gctgctcgcc gcgaccgatc
      241 ggcgttaccg cgtcgcggcg cgcgagccgc gccccgctgc ctatgccgcc gaggccggcg
      301 cggcgcgctg agaactaccc gaatgaaacc gcagatgaac acagataaac acagatactc
      361 aaatccagtg ccatgttagg tagaggatct cgtctgcgtt cacccgtgct tgtcttcggt
      421 tggtccggaa tccggttttt cgaagccgat tctagccgtt gcttcgcgaa gcacactata
      481 gctgtcgata aacaccgatg aatcctggcc gttctgtttt tgatcttatt tgcgttcatc
      541 tgcgtttatc ggcggttccg ttctttcttc atacatgacc cgctatctcc tgccgctcgg
      601 cgtcttcgcc cttttggtgc tgctgctcgg agtagggctc tcgctcgacc cgcgcgaagt
      661 cccctcgccg ctcatcggca agcccgcgcc cgcgtttcag ctgccgcaac tcgcccagcc
      721 cgagaagtcg ttttcgccca aagagctgca gggcaaggtg tgggtgctca acgtgtgggc
      781 ctcgtggtgc gccgcctgcc gcgaggagca cccggtgctg accgatctcg cccggtcggg
      841 gattgcgccg gtgtacgggc tcaactacaa ggacaggcgc gacgaggcgc tagcctggct
      901 caggcgcttc ggcgatccct accgggcctc gctcttcgac gcggacggcc gggtcggcat
      961 agactacggc gtctacggcg tgcccgagac ctacgtgatc gacaagcagg gtgtgatccg
     1021 ctacaagcgc atcgggccgg tcacgaccga gatcgtgcag aagaaaatcg cccctctgat
     1081 ccagcaactc aacggcggtt gagagtaatg cgtgataagt gataggtgat aagtgatgag
     1141 atccagggcg attttcttta tcctgatcct cctgtttgca ccctgggcgc atgccggcga
     1201 ggcgaaaccg gcggccgatg atccggtgct cgaggaaagg atgatggcgc tcgccgccga
     1261 gctgcgctgc ctggtgtgcc agaaccagac gctcgccgat tccaacgcgc ccctcgcggt
     1321 ggacctcagg aaccaggtcc gtgagcaaat gcggcagggc aaaagcgagc gcgagatcat
     1381 cgactatatg gtcgcgcgct atggcgattt cgtgctgttc cgcccgccgt tcaagttgac
     1441 gacgctgctg ctctggaccg gcccgctcct gctgctcgtg gcgggactga tcgcgctctt
     1501 ctaccggctc gcgcgccgtc gcgccgagcc caaagcggag ctttccgacg aagagcgcgc
     1561 gcgtgccgcg gccctgctcg agggcgacgc cgatatggag cggcggtgac ggcggtcttc
     1621 tggattctcg cggcgctgct cgctgcggtg gcgctcgcgt tcgttctgcc cgccctgctc
     1681 gggctcaggc accgggccga atccgctgca gccgacgcga ccaacatcgc ggtataccgc
     1741 gatcaactga aggaactcga ggccgacctc gccgcgggca ccctcaaccg ggaacaatac
     1801 gacgaggcgc gccgcgaaat cgagcggcga atgctcgagg acgtgcgggt cccgaagacg
     1861 acggcgcacg ccgcgaaatc cgggcgggag gctgcgatcg ccgccgccat cgcagtgccg
     1921 ctcgtgtccg tcctgctcta tctcgccgtc ggcaatccga aggcgctggc cccccgacaa
     1981 gatggcggcg gcgcggcgca cgaactcacg gcgggacaga tcgaagctct ggtcgagcgg
     2041 ctcgcggcgc gcatgcggga caatcccgaa gatgcccagg gctggatcat gctcgggcgc
     2101 tcctacaccg tgcttggacg cttcgccgat gctacccaag cctatgccca agccgctgca
     2161 cgtctgccgc gcgatgcaca ggtcctcgcc gactacgccg acgcgctggc gatggcgcag
     2221 ggaaagcgcc tagtgggcga gcccgaacgg ctcattgcgc gtgcgctcgc gctcgatccg
     2281 gacaatctca aggcgctcgc gctggcgggc accgtcgcgt tcgagaaaaa agactacgcg
     2341 agtgcggtgc gccactggga gcggatgctg ccgctcgtgc cggggaattc ggaggtcgag
     2401 cgctctatca aggcgagcat cgccgaggcg cgctctttgg gcggcggcgg gcagccggcg
     2461 cgcagtgcgg cgccgggcaa gggcgtgcgc ggagtggtca aactcgcgcc gcagctcgcg
     2521 gcgaaagccg cccccaccga caccgtgttc atcttcgtgc gcgcggccga gggaccgcgc
     2581 gcaccgctcg ccgtcctgcg caagcaggtg cgagacctgc cggccgagtt cgcgctcgac
     2641 gacacgatgg cgatggctcc gggcatgacg ttgtcgagct tcccgcgggt cgtggtaggc
     2701 gcgcggattt cgaaaaccac caacgcagcg ccgcagcccg gcgatctcca gggaatgagc
     2761 ccgcccgtcc gcaacgacgc gagcggcgtc accgttgtca tcaacaccga aacgcgctga
     2821 taacagtgaa acacgcgggc tagccccgcg gagcgctgag atccttgctt gaagacggcg
     2881 ccataccctg aatcacgcct tgttgctcaa gttcggttaa tttgcggtac aggctcgaca
     2941 ggccgatccc gagcctctgt gcagcccgcc tgcggtcgcc gccagcgtca tcgattgcgc
     3001 gcagaagaat ttcggcttcg accctccgca actgctcgcg cagatcccct tccacaccgt
     3061 tcgccgtagc attgggcatt tgaggtgacg ttgcactgac gacc
//
LOCUS       DBNGWZ010000280         6471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_157067, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000280 DBNGWZ010000000
VERSION     DBNGWZ010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_157067"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..799
                     /locus_tag="AC16CX_08210"
     CDS             335..799
                     /locus_tag="AC16CX_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3012531.1"
                     /translation="MLKRISSWSWLLWVALPLQAVMAQALYKSTMPDGRVVYGDKPAP
                     GAVKVEERRPDTAKKGVAPPVARETAVLKQMENERKARESTQDRVRRAEIALHDAEVA
                     LAMGKEPQPSERQGTAGKGQRLTDAYWDRQKRLEEAVEQARRNLEQVRSASR"
     gene            824..988
                     /locus_tag="AC16CX_08215"
     CDS             824..988
                     /locus_tag="AC16CX_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012532.1"
                     /translation="MGDGVIDLPLIRSWMEAAGYRGFHEAEIFSARNWWKRDPDEVLG
                     VIKRRHQECC"
     gene            complement(1269..2210)
                     /gene="arcC"
                     /locus_tag="AC16CX_08220"
                     /pseudo
     CDS             complement(1269..2210)
                     /gene="arcC"
                     /locus_tag="AC16CX_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(2328..4535)
                     /locus_tag="AC16CX_08225"
     CDS             complement(2328..4535)
                     /locus_tag="AC16CX_08225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012533.1"
                     /translation="MKMETFSRRDFLKTSGALVVTFSLAHPLAQAAEKGKAPAKTVAL
                     DQVDGFIAIDSKGNVTVYSGKVDLGTGVFTALTQMAAEELYVPFESVALVQGDTALTP
                     DQGNTWGSLSIQIGGMQIRQACATAREALLAQAAQKLGVAKGDLSVKDGVITAKSSGK
                     RVSYGQLVGGRNFEMKVDGKAPLKPASEYTIVGKSVNRIDIPGKVTGKFTYIHDFKRP
                     GMLHARVVRPAAMKAELQSLDDSAAKKIPGYVATVRKGSFVAAVAKNEWAAIRGADAI
                     KASWSRWEGLPEKARTWEHVRNTKVVRDEVFQNVGDSAKAMPAGARQLSATYDFAVHT
                     HGSMGPSCAVAEYKNGKLSVWTSSQATHMLRRQLAKMFDMKTDDIRCVYLEGSGCYGR
                     NGHEDAAADAALIAKELGKTVRVQWSRADEHGWSPKGPPTLLENRAALDADGNVVAWE
                     ITGYMPNRPKGFEVTLVAAELSGLPKDTAFPGNIFQSLAIPYAFPNIKATARWLAETP
                     FKPSWIRTPGRMQNTYANECFMDECAAAAGADPVEYRLRNLKDARGAECIDRCVRLAN
                     WTPRGKTPRDKGEIARGRGFSYIKYELVRTYVAVVADVSVNRRTGQVKVDRFHVAHDC
                     GQIINPDGLRNQIDGNVVQTVSRTLIEDLKWDRSRVTSLDWETYPILRFPDVPAVVID
                     LIDRPTERPWGAGEPTAAVVPSAIANAIYDAVGVRMRSVPFTPDKVLMALKSA"
     gene            complement(4549..5022)
                     /locus_tag="AC16CX_08230"
     CDS             complement(4549..5022)
                     /locus_tag="AC16CX_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012534.1"
                     /translation="MANNIQLTVNGKSHSLRVDDPQMPLLYALRNDLDLHGPRFGCGL
                     AQCGACTVHVDGKAVRSCRYPVSRAAGKKVVTLEGLGTPQKLHPLQQAFIQEQAVQCG
                     YCVNGMIMQAAELLAKNKRPSEDEIRQALADNLCRCGTHLRIIQAVKRASELMTA"
     gene            complement(5136..6167)
                     /locus_tag="AC16CX_08235"
     CDS             complement(5136..6167)
                     /locus_tag="AC16CX_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012535.1"
                     /translation="MNNKAEGESRKAKGYPTPRGRSYFRLPSSAFLLCCALAASAALA
                     QPYPTRPIRFVVPSAPGGGTDITARIVAPKLSEYLGQQVVVENRAGAGTMIGGEAVAR
                     AAPDGYTLLVGISTLAINPAMYRKVPYDALRDFAPITQLVSLSNLLVTHPSLPAKTVK
                     ELIAFARTRPGQIHYASAGVGTNPHLSVELFLSLARLKMVHVPYKGSGQGIVDVVAGH
                     VPVMMPSILTGLPHVKNGRLRGLGVTGGKRAGGAPDIPTIAEAGVPGYEAVQWFGVLA
                     PAGAPREIIARLHSEIVRVLQNADIRQRFSSDGADPVGSSPEEFAAFLRSETVKWAKV
                     VKDAGIQPE"
BASE COUNT         1001 a         2143 c         2195 g         1132 t
ORIGIN      
        1 ccgagcccga cacgccggtc acgacgatca gctcgctcag cggcagctcg agcgtgaggt
       61 tcttgaggtt gttctggcgc gcgccgcgga tgacgatggc gccgccgtgc atctcggaca
      121 tcgggaggtg acgcggttgg ggtgccgggc gccgacgcgc cccggctgcg aaaagcgcca
      181 ttctaaccgc acctgccgtc ttacgcgcgc actcaggaag cagccgcgcg gcggaagttc
      241 cgccgtccac ctgaacatgc gagaatgact tcccggtgaa cgcacggata aggcggccct
      301 gcgccaggac gcgggccggc gaggaggtga aatcatgctg aaacgcattt catcatggag
      361 ttggctgctt tgggtcgcgc tgccgctgca ggcagtcatg gcgcaggccc tctacaagtc
      421 caccatgccc gacggcaggg tcgtctacgg cgacaagccg gctcccgggg cggtcaaggt
      481 ggaagagagg agacccgaca ccgccaagaa aggcgtcgcg ccgcccgttg cccgcgaaac
      541 cgcggtgttg aagcagatgg aaaatgaacg gaaggcgcgc gagagcacgc aggacagggt
      601 gcgcagggcc gaaatcgcgt tgcacgacgc ggaagtcgcg ctcgccatgg gaaaggaacc
      661 tcagcccagc gagcggcagg gcaccgccgg caagggccaa cggctcaccg acgcctattg
      721 ggaccgccag aagcggttgg aggaggccgt ggagcaagcg cgccgcaacc tggagcaggt
      781 gcgttcggcc agcaggtagg cgacgcggca acccgccgcg cggatgggcg acggcgtgat
      841 cgaccttccg ctcatccgct cgtggatgga ggcggcgggc taccgcggct tccacgaggc
      901 cgagattttt tccgcccgca actggtggaa gcgcgacccc gatgaggtgc tcggcgtcat
      961 caagcggcgc caccaggagt gttgctgagc gcgccgatct acttcgccac cgtatttgac
     1021 ttcggatgcg gcggcgccgg cgcttcgccc gccggttgag acggtgtgat cggaggtatg
     1081 tcgaatggca cccacgcctg cggctcaggt ttcggggccg attgaggcgc tgccttgggc
     1141 gacagctgcg gcgaaggccg ggggtccggc ttgggagcgg atttcggtga cggcaggggc
     1201 ggcgtttgtg ccggccgcgc cgttacggct tgcggcgccg ccgccgctcg cgcagccggc
     1261 gaagccgctg ccggcttctc atgcggaacg aaatcgaggc tcacgcccgc cgaccgcacg
     1321 atggtgccgc gcaccccttc cagtatcgcg gcggcgtcct cgaggcgccc gatgccggcg
     1381 atcttgcctc ccgcccccac gaaacggcat gccgcctcga tcttgggctt catcgaaccc
     1441 gaggcgaacg cgtgcttgcg cagctcgtcc ggcatcgtct catggatcgg atgttgatga
     1501 ggcgtgcccc agccggtgta aacggcttcg acatcggtga gcagcagcaa cgcatcggcc
     1561 cccagttggt gcgcgagcag cgccgcagcg agatccttgt cgatcacggc ttccgctccg
     1621 tgcacggcgc ccgagggcaa tgtcacgacc gggatgccgc cgccgcccgc gcagaccacg
     1681 atcgcgcccg ccgccatgag cagccggatc gcggccagct ccttgatgcg tatcggctcg
     1741 ggcgagggca cgacgcgccg ccatttcggg ccgtcgggcg ccatggccca gccgcgctcc
     1801 ttcgcaagcc gttgcgcgtc ctgctcgctg aatgccgggc cgatcggctt ggaggggttc
     1861 ttgaatgccg ggtcgttcgg attgacctcg acctgggtga gcagggtgac gacctggcgt
     1921 ttcggcaagc ggctttccag ctcgatctcg atcagatagc cgatcatccc ttcgctttct
     1981 gcgccgagga tgtcgagcgg ataggggcgc acgccccggt acgcctccga ctggagcgcg
     2041 agcagtccca cctgtggtcc gttgccatgg gtcaccacca cggtatgatt gcgcgcgatc
     2101 gaggcgacgg cttccgccgc gtgcgcgacg ttggcacgct gcacgtcggc ttccagcggc
     2161 tcgccgcgct tcagcagcgc gttgccgcca agggcgatca cgactttcat ggcctggggc
     2221 atggctggca tgaacccatc taaccatgcc gggctcgcgg gaaggttgcg ctggatcaaa
     2281 aaaacgaggc ggcctgagcc gccccgcgta tccacccctg ctacgaatta ggccgacttc
     2341 agcgccatga gcaccttgtc cggggtgaac ggcaccgagc gcatccggac gccgaccgcg
     2401 tcgtagatcg cgttcgcgat cgcggacggc accaccgccg cggtcggttc gcccgcgccc
     2461 cacggccgct cggtcgggcg gtcgatgagg tcgatgacga ccgccggcac gtccgggaac
     2521 ctgaggatgg ggtaggtctc ccagtcgagg ctggtcaccc tcgagcggtc ccacttcagg
     2581 tcttcgatca gggtgcggct cacggtctgc accacgttgc cgtcgatctg gttcctgagc
     2641 ccgtccggat tgatgatctg cccgcagtcg tgcgcgacgt ggaagcgatc gaccttcacc
     2701 tgcccggtgc ggcggttgac cgagacatcg gcgaccaccg cgacgtaggt gcgcactagc
     2761 tcgtacttga tgtacgagaa gccgcggccg cgcgcgatct cgcccttgtc ccggggcgtc
     2821 ttgccgcgcg gcgtccagtt cgccagccgc acgcagcggt cgatgcactc ggcgccgcgc
     2881 gcatccttga ggttgcgcag ccggtactcg accggatccg cgcccgccgc cgccgcgcac
     2941 tcgtccatga agcactcgtt ggcgtaggtg ttctgcatgc gccccggcgt gcggatccac
     3001 gacggcttga acggcgtctc ggcgagccag cgcgcggtcg ccttgatgtt ggggaaggcg
     3061 tacgggatcg cgagcgactg gaaaatgttg cccgggaacg cggtgtcctt gggcaggccc
     3121 gagagctcgg cggcgaccag cgtcacctcg aagcccttgg gccggttcgg catgtagccc
     3181 gtgatttccc atgccaccac gttgccgtcg gcgtcgagcg cggcgcggtt ctcgaggagg
     3241 gtcggcgggc ccttcggcga ccagccgtgc tcgtcggctc ggctccactg cacgcgcacc
     3301 gtcttgccga gttccttcgc gatcagcgcg gcgtccgccg ccgcgtcctc gtggccgttg
     3361 cggccgtagc agccggaacc ctcgaggtag acgcagcgga tgtcgtcggt cttcatgtcg
     3421 aacattttgg cgagctgccg gcgcagcatg tgcgtcgcct gcgaggaggt ccacaccgag
     3481 agcttgccgt tcttgtactc ggcgaccgcg cacgagggcc ccattgagcc gtgcgtgtgc
     3541 accgcgaagt cgtaggtcgc ggaaagctgc cgcgcgccgg ccggcatcgc cttggcgctg
     3601 tcgccgacat tctggaacac ttcgtcccgg acgaccttgg tgttgcgcac gtgctcccag
     3661 gtccgcgcct tctcgggcag gccttcccac ctcgaccagc tcgccttgat cgcgtcggcg
     3721 ccccggatcg cggcccactc gttcttcgcc accgccgcga cgaagctgcc cttgcgcacc
     3781 gtggcgacgt agcccgggat cttcttcgcg gcggagtcgt ccagcgattg cagctccgcc
     3841 ttcatcgccg ccgggcgcac cactcgcgcg tgcagcatgc cggggcgctt gaagtcgtgg
     3901 atgtaggtga atttgccggt gaccttgccg gggatgtcga tgcggttgac cgatttgccg
     3961 acgatggtgt actcggaggc gggcttcagc ggcgccttgc cgtccacctt catctcgaag
     4021 ttcctgccgc cgacgagctg gccgtagctc acgcgcttgc cgctcgactt cgcggtgatc
     4081 acgccgtcct tcacggagag gtcgcccttc gcgacgccga gtttctgcgc ggcctgcgcg
     4141 agcagcgcct cgcgcgcggt cgcgcacgcc tgccggatct gcatgccgcc gatctggatg
     4201 ctgaggctgc cccacgtgtt gccctggtcc ggggtgagcg cggtatcgcc ctgcaccagc
     4261 gcgacgcttt cgaacggcac gtagagctcc tcggccgcca tctgggtgag cgcggtgaag
     4321 acgccggtgc cgaggtccac cttgcccgaa tacacggtga cgttgccctt cgagtcgatc
     4381 gcgatgaagc cgtccacctg gtcgagcgcg acggtcttgg ccggcgcttt gcctttctcc
     4441 gcggcctgcg ccagcggatg ggcgagcgag aaggtgacga ccagcgcgcc ggacgtcttg
     4501 agaaaatcgc gacgggagaa tgtttccatt ttcatgactg gtccctcctt acgccgtcat
     4561 cagttcggac gcgcgcttca ccgcctggat gatgcgcagg tgcgtgccgc agcggcacag
     4621 gttgtcggcg agcgcctggc ggatctcgtc ctcgctcggg cgcttgttct tcgcgagcag
     4681 ctcggccgcc tgcatgatca tgccgttgac gcagtagccg cactgcaccg cctgttcctg
     4741 gatgaaggcc tgctgcagcg ggtgcagttt ctgcggcgtg ccgaggccct cgagcgtgac
     4801 gactttcttg ccggcggccc gtgaaaccgg gtaacggcag gagcgcacgg cttttccgtc
     4861 cacgtgcacg gtgcaggcgc cgcattgcgc gagcccgcaa ccgaagcgcg ggccgtgcag
     4921 gtcgaggtcg ttgcggagcg catagagcag cggcatctgg ggatcgtcaa cgcgcaaact
     4981 gtgcgacttg ccgttgacgg tgagttggat gttgttcgcc atggtctcct ccttggcgca
     5041 ggtgggggga tcaatttagc cccgcttcat ggcgctggca agctgccggg gaagggcatg
     5101 attttgcggg agtacggggc ggtttgttta atccctcatt caggctgaat gccggcgtcc
     5161 ttgaccacct tcgcccactt cacggtttcg gagcggagga aggcggcgaa ctcttccggc
     5221 gagctgccta ccgggtcggc gccgtcgctc gaaaagcgct gcctgatgtc ggcgttctgc
     5281 agcacgcgca cgatctcgct gtggaggcgg gcgatgatct cgcgcggcgc gccggccggc
     5341 gcgagcacgc cgaaccattg caccgcttcg tagcccggca cgcccgcttc ggcgatcgtc
     5401 gggatgtcgg gcgcgccgcc cgcgcgcttt ccaccggtga cgccgagccc gcgcagccgg
     5461 ccgttcttca cgtggggcaa gccggtgaga atgctcggca tcatcaccgg cacgtgcccg
     5521 gcgaccacgt ccacgatgcc ctggcccgag cccttgtagg gcacgtggac catcttgagc
     5581 cgggccaacg agagaaacag ctccacggag agatgcgggt tggttccgac gccggccgag
     5641 gcgtagtgga tctgtcccgg ccgcgttctc gcgaacgcga tcagctcctt caccgtcttc
     5701 gccggcagcg aggggtgcgt gaccaggagg ttggagagcg acaccagctg cgtgatcggc
     5761 gcgaaatccc tgagcgcgtc gtacggcacc ttgcggtaca tcgccgggtt gatcgcgagc
     5821 gtgctgatgc cgacgagcag cgtgtagccg tcgggcgccg cgcgcgcgac cgcttcgccg
     5881 ccgatcatcg tgcccgcgcc cgcacggttc tcgacgacca cctgctgccc cagatactcg
     5941 gagagcttcg gcgcgacgat gcgcgccgtg atgtcggtgc cgccgccggg ggcggacggc
     6001 acgacgaagc ggattggccg cgtggggtac ggctgcgcca gcgcggcact tgccgcaaga
     6061 gcgcagcaaa ggagaaaggc ggaagaagga aggcgaaagt aagaacgtcc gcgtggcgtt
     6121 ggatatcctt tcgcctttcg actttcgcct tccgccttgt tattcatcct tcctccctca
     6181 tgcttcacct tgctttgtgg gccgcgcctt gccccggcgc atgcgcgtga ctaaatgcca
     6241 gccagaaaac cagaacgtaa ccgcaggtga acgctgataa acacggatag ttcgattcct
     6301 caagccgtca tcggcagagg tcgtcatcgc gtttatccgg gctcatccgc ggctcgcctt
     6361 gctttattat ctctgagaac tgccaggaac gaatctcgaa aatttacacc agaattgaac
     6421 cgcatataat ctgctgatta acgccgaagt aaagcaaaag aattcatcgg g
//
LOCUS       DBNGWZ010000281         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3431826, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000281 DBNGWZ010000000
VERSION     DBNGWZ010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3431826"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1089)
                     /locus_tag="AC16CX_08240"
     CDS             complement(121..1089)
                     /locus_tag="AC16CX_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012536.1"
                     /translation="MRRFLHIAILFLASLAGGALCAQSYPTRPIRFVVPFAPGGPTDI
                     LARAVGQKLTEAFGQTIVIENRGGAGGNIGAELVARSPADGYTMLMGATSTHAINPSL
                     YHKLGFDPVKDFIPVSLVANTPSLLAVHPALPVRTIKDLIALAKARPGQLTYASAGNG
                     SSNHLAGVLLSMMAGIDLVHVPYKGSGPALVDVISGQVPMMFNNTASVMPHVTAGKLR
                     AVALASLERSALLPDIPTVAESGLHGFEVRSWHGVFVPAGTPREVVNRLSTEIVRAVR
                     AADVKSRLNAQGVELVGSSPEEFAEFVKKELAKWAKVVRQSGARAD"
     gene            complement(1123..1707)
                     /locus_tag="AC16CX_08245"
     CDS             complement(1123..1707)
                     /locus_tag="AC16CX_08245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3012537.1"
                     /translation="MTTRSIYLVLDMENDLVHADGPNGKSPAGQQVQARRIVAKTAAA
                     ISRARAAGVRIGYVRVGFSPDYRECPPKSPVFSAARKNSLFKLGTWGTEIHPDITSQP
                     EDFQIVKHRVSPFYSTNLEAILRANGITRIYCSGFSTNAVVQATVREGHDRDYEIVVL
                     EDCCAAASEEEHKAAITSLKRFCTIITSTEVVFD"
     gene            complement(1723..>2193)
                     /locus_tag="AC16CX_08250"
     CDS             complement(1723..>2193)
                     /locus_tag="AC16CX_08250"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3012538.1"
                     /translation="SATREIMELRVTLESRLAAAAMQNMTPQDEADLWKLQRDFEKAI
                     AQGDRPSVRAMNYRFHSHLYSLARRPQTLHFVQVLWAKYPFDLINLIANRPARAAKEH
                     AAILRVIARRDEKALIAAVRDHIEAGWRELKKDLESRKSRATKPAARHRTRQHH"
BASE COUNT          365 a          675 c          718 g          435 t
ORIGIN      
        1 ccgccaagaa tgtctttgca caggtcgaag cccttgaccg tgatccggtc ggtcgtgctc
       61 caggcgatgt cggtgcgcag tgttttcctc ttgctcatgt gatgctccct ggcggttatc
      121 tcagtcggcg cgcgcgccgg actgcctcac gactttcgcc cacttggcga gttccttctt
      181 cacgaactcg gcgaactctt ccggcgagct gccgacgagc tcgacgcctt gcgcgttcag
      241 ccggctcttg acgtcggcag cgcgcacggc gcggacaatt tcggtgctca aacggtttac
      301 tacttcccgt ggcgttccgg ccgggacgaa cacaccgtgc caggagcgga cttcgaatcc
      361 gtgaagcccc gactcggcga cggtcggtat atcggggagc agggcggagc gctccaggct
      421 ggcgagggca accgcgcgga gctttccggc tgtcacatgg ggcatgaccg acgccgtatt
      481 attgaacatc atggggacct gcccggagat cacgtcgacg agcgcgggcc cgcttccctt
      541 gtaggggaca tgcacgaggt cgatcccggc catcatggac agcaaaacgc cggcgaggtg
      601 gttggagctg ccgttgcctg ccgaggcata ggtcaactga cccggacgcg ccttcgcgag
      661 cgcgatgaga tcctttatgg tgcgcaccgg cagcgccggg tgcacggcca gtagactcgg
      721 cgtgttcgcg acgaggctca cgggaataaa gtccttgacg ggatcaaagc cgagcttgtg
      781 atacaggctg gggttgatcg cgtgggtgct ggttgcgccc atcagcatgg tatagccgtc
      841 ggccggcgag cgagcgacca gctcggcgcc gatattgcct ccggcaccac cccggttctc
      901 gatcacgatc gtctggccga acgcttcggt aagcttctgg cccacggcgc gcgcaagaat
      961 gtcggtggga ccgcccggcg caaagggcac cacaaagcgg atgggcctgg tgggatagct
     1021 ctgagcgcac agcgcaccac cagcaagcga tgccaggaaa aggatggcga tgtgcagaaa
     1081 gcggcgcacg gtgttctcct gttataacga tgatcacgtc gcctagtcaa acactacctc
     1141 ggtcgaggtg atgatggtgc agaagcgctt gaggctcgtt atcgcggcct tgtgctcctc
     1201 ttcgctcgct gccgcgcagc agtcctcgag caccacgatc tcgtagtcgc gatcgtggcc
     1261 ctcgcgcact gtcgcctgca cgaccgcgtt ggtcgaaaac cccgagcagt agatccgcgt
     1321 gatgccgttc gcgcgcagga tcgcctcgag attcgtggag tagaaagggc tcacacggtg
     1381 cttcacgatc tggaagtcct cgggctgcga ggtgatgtcc ggatggattt ccgtgcccca
     1441 ggtgccgagc ttgaagaggc tatttttcct cgcagccgag aagacaggcg atttgggcgg
     1501 gcactcgcgg tagtccggtg aaaagccgac ccgtacgtat ccgatcctca cgccggcggc
     1561 gcgggccctg ctgatcgccg ctgcggtctt tgcgacgatt ctccgggcct gcacctgttg
     1621 cccggcgggc gacttgccgt tgggcccatc cgcgtgcacg agatcatttt ccatgtcgag
     1681 gacgagatag atggagcggg tcgtcatggc tgcgagtgcc tcctaatggt gttgccgcgt
     1741 gcggtggcgc gccgccggct ttgttgcacg tgatttgcgg ctttccaggt ctttcttgag
     1801 ctcccgccag ccggcttcga tgtgatcgcg caccgccgca atgagcgcct tctcatcccg
     1861 gcgcgcgatc accctgagaa tggccgcgtg ctccttggct gcgcgcgcgg gacgattggc
     1921 gatcaggttg atgagatcga acgggtactt cgcccacagg acctgcacga aatgcagcgt
     1981 ctgcggccgc cgcgcgagcg agtagagatg gctgtggaag cggtagttca ttgcgcgcac
     2041 cgaaggccgg tctccctggg cgatcgcctt ttcgaagtcg cgctgcaatt tccagagatc
     2101 ggcttcgtcc tgtggcgtca tgttctgcat tgccgccgcc gcaagccggc tttcgagcgt
     2161 cacgcgcagc tccatgatct cacgcgtggc gga
//
LOCUS       DBNGWZ010000282         1878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1038630, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000282 DBNGWZ010000000
VERSION     DBNGWZ010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1038630"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16CX_08255"
     CDS             complement(<1..347)
                     /locus_tag="AC16CX_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012539.1"
                     /translation="MHVRSEFTKSTAGAMPGPVFTGCSMASAAQARAPAKGKAKPAAG
                     RRREVKVGGKRVKTIDVHAHCVIPEAMRLLGLRLEDQRGPGIDQVGERRIREMDEQGI
                     DMEALSINPQWYRA"
     gene            complement(466..1458)
                     /locus_tag="AC16CX_08260"
     CDS             complement(466..1458)
                     /locus_tag="AC16CX_08260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR3012540.1"
                     /translation="MTTGKKRLLLPKTMARPGWDLAKSRADVEAIAFSPGAPAPEFRQ
                     LLRDADGVALTSTPLSAADIEAAPRLRAVGRIGVGYDAVDVEALNRRGIPLMTCGTDN
                     SPTVAEYALFMMLALAKLGARMDAIPRQGRWSQRYDELNSELLGKTLLIIGFGRIGTR
                     IAKRSLAMEMRVEVYDPYVPAAAVRAAGCEPVADLDAALPRADFVTLHCPKTKETTGM
                     FDAARIARMKPSARLINTARGGLVDEAALYAALAAGKLAGAGIDVFSPEPPLTDNPLF
                     TLPNVIVSPHMAGNSCESLDRKSLTVVRNLLSVLDGRPNTEYVVNPEALGGRRK"
     gene            complement(1540..>1878)
                     /locus_tag="AC16CX_08265"
     CDS             complement(1540..>1878)
                     /locus_tag="AC16CX_08265"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3012541.1"
                     /translation="ATSTIHIEGGRTMLWAISCVDKPNTAAIREKVLQPHRDYLQSRK
                     KILVLAGATQTDDGTQAIGSLFIVNLDSRAKAKAFSDGDPFTKEGVFKSVTITRMRKG
                     QWNPEAAEGA"
BASE COUNT          269 a          630 c          615 g          364 t
ORIGIN      
        1 gcccggtacc actgcgggtt gatgctgagc gcctccatgt cgatgccctg ctcgtccatc
       61 tcgcggatgc gccgctcccc gacctggtct atgcccgggc cgcgctgatc ctccagtctc
      121 agtcccagca gcctcatggc ttcggggatc acgcagtgcg cgtgcacgtc gatggtcttg
      181 acgcgcttgc ctcccacctt cacttcgcgg cggcgcccgg cggccggctt tgccttgccc
      241 ttcgccggcg cccgcgcctg cgcggcgctc gccatgctgc aaccggtgaa aaccgggccg
      301 ggcatcgcgc cggccgtgct cttggtgaac tcgctgcgaa cgtgcatctt cttctccctg
      361 gttgattatc ttgaaatccg tggatcgtgg gtcgtgggtc gatctgcccg gtagggcgtg
      421 agcagtaagt agaataagcc cgccttacca gtcacgcttc atcagtcact tccttcggcc
      481 gccgagcgct tccgggttga cgacgtattc agtattcggc ctgccgtcca gcacgctcaa
      541 gagattgcgc acgaccgtca gcgacttgcg gtcgagcgat tcgcacgagt tgccggccat
      601 gtgcggcgag acgatgacgt tcggcagcgt gaagagcggg ttgtccgtga gcggcggctc
      661 cggggagaac acgtcgatgc ccgcgccggc gagttttccc gcggcgagcg cggcgtagag
      721 cgcggcctcg tccaccaggc cgccgcgcgc ggtgttgatg agccgggcgg agggcttcat
      781 gcgcgcgatc cgtgccgcgt cgaacatgcc cgtggtttcc ttggtcttgg gacagtggag
      841 agtgacgaaa tcggcgcgcg gcaacgcggc gtcgagatcg gcgaccggct cgcatcccgc
      901 cgcgcgcacc gccgccgccg ggacataggg atcgtacacc tcgacccgca tctccatggc
      961 gagggagcgc ttggcgatgc gcgtgccgat gcggccgaag ccgatgatga gcagcgtctt
     1021 gccgagcagc tcggagttga gctcgtcgta gcgctgcgac cagcggccct gccgggggat
     1081 ggcgtccatc ctggcgccga gtttggcgag cgcgagcatc atgaacagcg cgtactcggc
     1141 gacggttggc gaattgtcgg ttccgcaggt catcagcggg atgccgcgac ggttcagcgc
     1201 ctcgacgtcc accgcgtcgt agcccacgcc gatgcggccg accgcgcgca accgcggcgc
     1261 ggcctcgatg tcggccgcgc tcaacggcgt gctggtgagc gcgacgccgt ccgcatcgcg
     1321 cagcagttgc cggaactccg gcgccggagc gcccggagag aatgcgatgg cttccacgtc
     1381 cgcgcggctc ttagccagat cccagccagg gcgcgccatc gtcttgggca gcagcagtct
     1441 tttcttgcct gtcgtcatcg gaaattccta tgcgtcactc gacactcgtc actcgtcact
     1501 cgacactcga cactcgttac tcgtcactcg tcactgcagt caggcccctt ccgccgcttc
     1561 cggattccac tgccccttcc gcatgcgcgt gatggtgacg ctcttgaaca cgccttcctt
     1621 ggtgaacggg tcgccgtccg agaaggcctt ggcctttgcg cggctgtcca ggttgacgat
     1681 gaacaggctg ccgatggcct gcgtgccgtc gtcggtctgc gtcgcacccg caagcaccag
     1741 gatcttcttc cggctttgca ggtaatcgcg gtgcggctgc aagacctttt cgcggatcgc
     1801 ggcggtgttc ggcttgtcaa cgcaggaaat ggcccaaagc atggtgcgtc ccccttcgat
     1861 gtggattgtc gatgtggc
//
LOCUS       DBNGWZ010000283        21739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1038651, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000283 DBNGWZ010000000
VERSION     DBNGWZ010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 21739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21739
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1038651"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC16CX_08270"
     CDS             <1..503
                     /locus_tag="AC16CX_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3012542.1"
                     /translation="EALALSHEIAVMNEGTIMQVGTPREIYERPRSRFVADFIGRTNL
                     LEARVVAVDGAGHYRLGTALGELHAASADALKAGDAVAISIRPEDIELSEQRPQGANV
                     CAGTVSAKVFLGDTVDFQVAVGDQMLLATAHPNLRTPVGDPIFLRISTEKCIALAGDK
                     SVIGDK"
     gene            642..1781
                     /locus_tag="AC16CX_08275"
     CDS             642..1781
                     /locus_tag="AC16CX_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012543.1"
                     /translation="MAKNAMVSEEMAKKFATEKETPYTRWVKAEGLDILPSHYVPNLH
                     TVELKPWARRGGRGMYFNHEASRTSNDCYVCEIPAGGKLAPQRQLFEEMVMILSGRGS
                     TAVWNDTGGRITFEWKAGALFAIPVNAWHQHFNGSGQEPARYVAVTNAPIVINLYDDV
                     DFVFNTKYDFRNRFAGEPDYFAPKNEQKGFLLDTNFVADAVNLPLIAAKERGAGGGHI
                     RFNMAKGSMNSHISQFPVGTYKKAHAHGPGAHVIILSGEGYTLMWPEGEEPRRYEWNV
                     GAMIVPPTMWFHQHFNTGTTPARYLAFKYEGTAIRNAQGVPKAWISRRLGGDQIDYAD
                     ESPVVRRWFAEALAKHDINSRMDEAYQAELASLQQGTLVAAGGDD"
     gene            1819..2916
                     /locus_tag="AC16CX_08280"
     CDS             1819..2916
                     /locus_tag="AC16CX_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012544.1"
                     /translation="MRKAEPAQSAAEQKHFRLSPFAFRLAAALLLAAPAAAPASAQPS
                     VAEVAGLQGPDRMQRLVEGAKKEGTITIYTSATVEDMKVLSAAFEKKHGIKVNVWRAS
                     SENIVRRGISEMRGGRNEADIFETNGPDMESLHREQLFQEVKSPHLADLIPGAVAPHR
                     EWVGARINIYVMTYNTRLIRKDELPRSFEDLANPRWKGMLGIEASDPAWFSTVVSDLG
                     EAKGLKLFRAIVAANGVSVRKGHSLLTNLVASGEVPLALTTYHYRAEQLKKKGAPIDW
                     FILPPAVARFQGMGLARRAPHPHAAVLFFDFMLTDAQELLAQRNFTVTSRKIDSPAHK
                     MPLKFVDPRLVLDESEKWGKLYEEIVVKQSR"
     gene            2935..3483
                     /locus_tag="AC16CX_08285"
     CDS             2935..3483
                     /locus_tag="AC16CX_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012545.1"
                     /translation="MGKASHYHDKNDRIFVRAIQGHYNLKEELARLRSMPRVVRGKEL
                     KFQDGPQSYSKHFVEPVDGITQTLHIHLEEYAPGGRTQKHGHVNEAAFYILDGRGYEI
                     HDGVRYDWQAGDIAIVHNNCVHQHFNGDPDKPARLLVLKSKPMFLWMNMLFQKTVIPK
                     PKEPAPGAEGYEPRNDEVDYNH"
     gene            3545..4159
                     /locus_tag="AC16CX_08290"
     CDS             3545..4159
                     /locus_tag="AC16CX_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014136.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012546.1"
                     /translation="MAWGESKAWMRGTASGKLYQNLLDDAKGAPARNAKRKKIVHPEE
                     MPWEMSRQGLLKHLLNEQMNTRMETVDAYMQIIPPGSRSGRHRHLAEECLFVVEGRGY
                     DLHQDCDVEITDTYHWKPQDEIKRYEWEAGDVIYIPPNTIHQHFNADPDRPVRLISAI
                     NRIYKNCGLNDLEQLEDAPEYDPKVVLTAEVIKRYLAAKVKEPA"
     gene            complement(4143..4295)
                     /locus_tag="AC16CX_08295"
     CDS             complement(4143..4295)
                     /locus_tag="AC16CX_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012547.1"
                     /translation="MKTVKKTGAAVIAAAPLFGYDTGVEASASKPEARYHPVCMLTPG
                     RFRLVP"
     gene            4311..6098
                     /locus_tag="AC16CX_08300"
     CDS             4311..6098
                     /locus_tag="AC16CX_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012548.1"
                     /translation="MSKPSTKPKENPAPSAAANRRWASDVIVDMLHRYELPYAALNPG
                     ASYRGLHDSLVNYGGNKPYMMLCQHEETAVQIAHGYAKASGRPMVAILHNLVGLLHAN
                     LAIYYAYIDRAPIFIIGATGPMDETKRRPRIDWTHTAQVQGEAVRAYTKWDYQPTVVD
                     GVPESFARAYSVMMTEPQGPVYLCYDAWLQEQPLDHDVPLPPPTSMPVPSPFTADPTA
                     LARAAEMICEAQRPVILAEFVGRDPKGFDQLVALAETLGAPVYDVNSRLNFPGCHPLN
                     LSMVKDVFKDADLILCLDVRDWERATTELVSQTRTLTSIEPPDCVWIDIGFGDLELSS
                     WAMDYQRLKHAQLRILADTALAIPALTDLLRKRIAASPGLKKTIDERARGTAERHREA
                     RAKWAQQAREDWDATPIPLPRLASEVWDVIRHEDWVLTAGTLEDWTRKLWDFDRPYRH
                     PGKSLGTATQIGLSLGVALAHRDAGRLVVDLQPDGDLMFDAGALWVAVKHKIPMLIVM
                     YNNRAYYNDWEHQIRMARQRGTPIERAHIGMDLEEPTVDFAGLARSMGWYAEGPIERG
                     KDVGPALKRAIARVKAGQPALLDTITQKR"
     gene            6335..7636
                     /locus_tag="AC16CX_08305"
     CDS             6335..7636
                     /locus_tag="AC16CX_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3012549.1"
                     /translation="MVTVAQNDYLTRTGPGTPMGQLFRRYWLPVLLEQELPGLDCPPV
                     RVKLLGERLLAFRDTQGRLGLISEFCAHRGVSLWFGRNEESGIRCPYHGWKYDVSGQC
                     LDVPSEPPENGFASRIKLTSYPLIERGGVIWAYLGPPESKPHEPAYEWTLVPPAHRYV
                     SKRLQECNYLQAMEGALDSIHSTFLHRHSVADDPLLKRDPQSLELIRGDAKPAFVPTV
                     SPAGLYIATRRNAGEDRYYWRVTHWLMPCFSFFPPYGDNPFGGHAFVPIDDENCWTFS
                     IDYHPRRALSRTEHDAMHAGKGIHVRLIEGTFTPVANKRNDYRIDRAGQKTKKTFSGV
                     FDVGVQDAAVQESMGPIQDRAREHLVSSDNGIIMTRRRLMDAADAVGRGIAPPGLDPA
                     EQRERALSMVTPRELPLLDAIARYSDGQRASSPGDREVERP"
     gene            7795..8628
                     /locus_tag="AC16CX_08310"
     CDS             7795..8628
                     /locus_tag="AC16CX_08310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012550.1"
                     /translation="MNYEASDRIQNVGVIGAGRMGQPIIGHLARKGFSVQVHDIDPGK
                     QAAIEARGGRWVADAAALARQSDAILICVGYDHELRELLSEDGILNHARRGAIIAVLS
                     TVHPETVRELAERGRARGLHVVDATMCRGAWAADEGTLLSFVAGTAEIVARLRPVLAA
                     FSSDIVPTGDIGSAQVAKAVNNMVMWACLVANHEGLALAHRYGLDIETVRKALLTTHA
                     ANAALEHWGRQSMAWADDDMEIVAAMAKEKGLALRQAKAVQEICRTLKPRRYQLDKFG
                     L"
     gene            8726..10123
                     /gene="soxC"
                     /locus_tag="AC16CX_08315"
     CDS             8726..10123
                     /gene="soxC"
                     /locus_tag="AC16CX_08315"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HIR3012551.1"
                     /translation="MTASFDEKREGLEHALRHIGESVTEADVQAFIHQETRAREEPGA
                     PVTRRRFLSGVGMAAATAGVAALSARPADAKAPPGAIEYPVQADSTREQGRVMGEDGG
                     YGSRSQFETEVRAWQPTRTASFTPMQKGHGIVTPSGLHYERHHGGIPNIDPRKHSLII
                     HGLVERPVKFSVADLMRFPSVSRIYFMECSGTSGSELMKATMPTVQRTHGLVSTSEWT
                     GVPLSTLFKQVGLRNNGAWVLAEGADAAVMTRSVPLDKCLADALIVYAQNGEAIRPEQ
                     GYPIRLFLPGWEGNISIKWLRRLEVGDKPYQTREETSKYSDIITKTGKARQFTFTMEA
                     KSVITFPSGELKLPGPGFYEITGLAWTGRGKIARVEVTTDGGKTWHLASLQDPILPIA
                     QTRFRYPWVWDGSPAIIASRATDETGYSQPSKEQLIAERGPLEVGLFYHMNAVQHWSI
                     DREGSVKNWHPLQKG"
     gene            10128..10697
                     /locus_tag="AC16CX_08320"
     CDS             10128..10697
                     /locus_tag="AC16CX_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3012552.1"
                     /translation="MGRFLVAGTAIALALAAMGAGYPEKFDFGAPATDAEIKVVAIAI
                     PPDGKWLPPGNGDWAAGKKVYDIQCAGCHGSDLLGVAGIKDMPAGAQLRLIGGRGTLT
                     SPKPVLTVESYWPYATTLFDYIRRAMPFFAPGSLSNDDVYAVSAYILGEAKIIDKSAV
                     MNGESLPKVKMPNRDGFIPDPRPERFGTR"
     gene            10734..10880
                     /locus_tag="AC16CX_08325"
     CDS             10734..10880
                     /locus_tag="AC16CX_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012553.1"
                     /translation="MRPDAGRRRAFHVLLAVASLLVALTSAAVQEICRTLKPKRFKLD
                     QYGR"
     gene            10927..11922
                     /locus_tag="AC16CX_08330"
     CDS             10927..11922
                     /locus_tag="AC16CX_08330"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012554.1"
                     /translation="MNRRHFRLSNSDSRLSLALAAACLLLAPPASAQDVWPSRPVRLV
                     VPSSPGGGTDTYARLLAQGLGDALKQQFVVDNRPGGGGNIGAEIAARAAPDGYTFLVS
                     SSPALVVNPSLFRSLPYSAERDFTPVARGVVSPLVFASHPSVPVKSLAALVALARRES
                     GSLSYGSPGVGSSSNLAVKMVEEAGKVRFLHVPYKGAAQAVLGLLRGEVAFMVTDIST
                     ALPHIHSRKVVPLAASHRSPHIPGTPTYAEAGFPSVDAYPSYSVVAPTGTPPAIVQRM
                     SAEIVGLMKSPAYRERLDARALIPVFDTPDEFAAVLKRERARYAEVIRRNNITAE"
     gene            11934..13064
                     /locus_tag="AC16CX_08335"
     CDS             11934..13064
                     /locus_tag="AC16CX_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012555.1"
                     /translation="MKSEVESRKAEVTAQIKPARPSADHYFRLSPFDFRLAIAALLAL
                     FAAAPALAQSLAELAAYSGADREQRLAEGARREGVLNLYTSLTVDDMAVLNAAFEKTT
                     GVKVRMWRASSEKIVQRALAEARAGRFDVDVIESNAPSLEILHREGLLQAVRSPHHAD
                     LIPAAVPAHREWVGARLNIFVQAYNTRLVRKEELPSSYSDLLHPRWKGRLGIEASDED
                     WFAAVVQSMGEERGLKLFRELAAANGLSVRRGHTLLTNLVVAGEVPLALTVYNFTAEQ
                     VKQKGAPLDWFVLPPAIARANGVAVARRAPHPHAALLYYEFMIGDEGQSILLKRDFVP
                     ASRKMDTELNRGPLTIIDPALIVDQGERWAKLYDEIIVKQGR"
     gene            13082..14152
                     /locus_tag="AC16CX_08340"
     CDS             13082..14152
                     /locus_tag="AC16CX_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3012556.1"
                     /translation="MTRKPFRLSPFAFRLVAAGLAVLALAPATASAQATAQSTLATYA
                     GADRFERIVAAAKKESSLMLYTTFAASNMEAVATDFERRYGVKVNSWRSGTLRVLQRT
                     LAEAKANRFEVDAVIFGSPEMEALYREQLLQEVRSPHHGNLVDGALPAHRAWSAVYLN
                     LFAHAYNTDKVRKEELPRTYADLLHPRWKGRLGVESQDQEWFLAVVKDMGEARGLKFF
                     QDLVAGNGLSVRTGHSLLNNLVVSGEVPLALTVHSYMPEQMKRKGAPIDWFVMEPAVV
                     RPNAVGVMRRAPHPNAALLFYEYMLSDGQKVLADLAYIPATRQGDSPLRNLRVRVVDP
                     VEVLDENDKWSKLYEDTLRGAR"
     gene            14182..15273
                     /locus_tag="AC16CX_08345"
     CDS             14182..15273
                     /locus_tag="AC16CX_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012557.1"
                     /translation="MIIDLDSHLREGYFLDEVYRLDGPLARFTPRKVGDGRSHATQFI
                     HSLEPGDARALAAFKHPYIYDPKNDPGLAERQRGGYDMARRLADLRKEGIDKQIIFPT
                     GIDIATKNAGELGAALARCYNDWVGNLVRGYEDVFMPCTVAPAGCPEQMAAELRRCVQ
                     EKEFKAGHLVCYCGPRNLDDASFHGYYAAAQELDVPLLCHPNSVGELTDRFNNFFKLH
                     IAGRPNNCTTALIALVAGGVFEKFPKLRVAFFECSAEWMLYWMHRMDDDWEWASRYPE
                     QTGGSALKMAPSEYIKRNCYVTCEADERYLGNAAAELGADHICMATDYPHFDSKFPET
                     VSGIRQRGDLTAPQKEMILGGTAAKLLRL"
     gene            15449..16045
                     /locus_tag="AC16CX_08350"
     CDS             15449..16045
                     /locus_tag="AC16CX_08350"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR3012558.1"
                     /translation="MKPCLITLFAGVFALSASAADAQEKPMRVRGTIVAVEGNVLALK
                     SRDGKDLQLQLTDKTAVAAARAIKLADLKKGDYVGSTTMKNAQGVLVAHEVHRIPRTV
                     KEGHGPWDLLPNSMMTNANVASVVMVADGEQLTLEYSGGSQTILVPAGTPIVETAPAD
                     RSFLKPGEYVFVAAQAGADGKITALRIQVSHGGVRPPQ"
     gene            16182..16760
                     /locus_tag="AC16CX_08355"
     CDS             16182..16760
                     /locus_tag="AC16CX_08355"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR3012559.1"
                     /translation="MKLLHPCLLFVVLALMAGAVEAQAPTRVRGTVSAFAGNLLSVKS
                     QDGRNTEIQLADNSVIVFTQPIALADIKPGDFLGVTSMKRDDGTLTAYEVRRFPKPLN
                     PGHRPFDGRNDQTMTNATVAATVQSASGRELTLTYEGGAQKIMVPESASISMLVPGER
                     SHLVPGAAVNLTATPGDDGKLTALRIQVSPPR"
     gene            16997..17641
                     /locus_tag="AC16CX_08360"
     CDS             16997..17641
                     /locus_tag="AC16CX_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR3012560.1"
                     /translation="MIYEFRTYDLKPHSLPEVEKRFGEAYETRKKFSQLAAFWHTEIG
                     PLNQVIHVWPYQDLAERARIRDAAVKESGGKWPPKTAEFMVSQRSDIFIPFDFSPEIK
                     PGKQGPFFEMRTYTYATGQLGNLTKAWAHAIPFRMKHGPVTAILYSELGGLNKFVHIW
                     PYKTLDERWAMRNKMRETGDWPPSVAAKKAGLPEYHLVQQENKILMPSAFSPLQ"
     gene            17757..19007
                     /locus_tag="AC16CX_08365"
     CDS             17757..19007
                     /locus_tag="AC16CX_08365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3012561.1"
                     /translation="MYMMELADRGMQDETRMMRDVTRKFVNEHVIPFVRQNWQQEWSM
                     NPDGRLPRKILEVADAIGIRTLGVPEEFGGTPLDPKTEVQTFAVISEEISRGDCGLSD
                     KMVQIWKVSVLLRNLAPRHLQELWFPRLVKDPTFLLAHCLTEPRGASDRWLPYNVPEA
                     AMQTKAVLKGDRWVVNGRKQFISNGYDAKLYVVYANTNPKVGMLQGTSSFLVPRDTKG
                     LTVQRCNETLGGRFMNNGELVFEDMELPKDHLLVEGNALSRAGIYFRPGKIIQGSKNL
                     GVGVRAFEETARYVQEYVQGGRILIKHQATAIRLAEMATKICATRGLLREAAAAVDAN
                     APDADVLCNMVKLYASEAVLEVCKHAMELHGGNGTMLDFGIEKLYRDASMFLHMDGTV
                     DVTKFKIVKALFPETAGKYAGPET"
     gene            complement(19111..19980)
                     /locus_tag="AC16CX_08370"
     CDS             complement(19111..19980)
                     /locus_tag="AC16CX_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012562.1"
                     /translation="MLVGFSPGGATDVAARIIGKKLSETWNQQVVIDNRAGAGGLLAF
                     EMAAKANPDGYTLLATSPSFAIQPSIAGKLPFDPIRDFAPITQMSAAPYILVLYPGLE
                     AKSVKELIGLAKANPGKLNYASGGIGSAQHLAAELFSLMAGIQMVHVPFKGAVSIPDI
                     IAGRVQMVFSGLPQALPHVQAGRLRAVGVTTPARWPAAPGVPTIAEAGVPGYDVTVWY
                     GILVTGRTPRPVVDKLNAEIVKTLQSADVRQQFAAMGLEPVGNPSAQFAAMIRAEIAQ
                     WAKVVKQAGIKPQ"
     gene            complement(20143..21228)
                     /locus_tag="AC16CX_08375"
     CDS             complement(20143..21228)
                     /locus_tag="AC16CX_08375"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR3012563.1"
                     /translation="MSEPRIGRRPKFAYVGCFTTEKRRARGKGIAVFRIDPATGAWTF
                     VEAFDAIPNPSYICLDRTQRFLYSAHGDGAEIGAYARDMQTGKLRFLNRQQTDGDNSS
                     TVMPDPGNRYVVLSNGPGVAVFPINEDGSLAPYTDLVIPQGNPGPYRDEQHGSHPHQA
                     TFDLTGRYVVVPDKGLDKVLVYRFDAARGKLAPCDPPFVKSRYGAIPRHITFHPTCPY
                     AYVVNEQDSTVNAYHWDTGRGALKPFQRVPTTPTTYVGDNMAAEIAILPSGKFLYASN
                     RGHDSIVIYSVSQSTGLIEPVGWESVQGRKPRFFGLDPGASCLYAANENSHTIVEFRI
                     DQETGKLTPTGQIIETGSPTCIVFATQ"
     gene            complement(21292..21624)
                     /locus_tag="AC16CX_08380"
     CDS             complement(21292..21624)
                     /locus_tag="AC16CX_08380"
                     /inference="COORDINATES: protein motif:HMM:NF023171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HIR3012564.1"
                     /translation="MPGLEVFGILVLGGIAWLWFDSLKAREAAIRAAREVCAAEGLQL
                     LDYTVAISGLRPARDERGSLTLQRTYDFEFSDTGNNRLKGSVVMRGHRVVVLNVGLRV
                     PPEVRTLH"
BASE COUNT         4087 a         7070 c         7042 g         3540 t
ORIGIN      
        1 ccgaggcgct cgcgctctcg cacgagatcg ccgtgatgaa cgagggcacg atcatgcagg
       61 tcggcacgcc gcgcgagatc tacgagcggc cgcgcagccg cttcgtcgcc gacttcatcg
      121 gcaggaccaa cctgctggag gcgcgggtcg tcgcggtgga cggggcgggg cactaccgcc
      181 tgggcacggc gctgggcgaa ctgcacgccg cctccgccga cgcgctcaag gcgggcgacg
      241 ccgtggcgat ctcgatccgg ccggaggata tcgagctctc cgagcagcgg ccgcagggcg
      301 ccaacgtctg cgccggcacg gtgagcgcca aggtgtttct cggcgacacg gtggatttcc
      361 aggtggcggt cggcgaccaa atgctgctcg cgaccgcgca ccccaacctg cgcacgccgg
      421 tgggcgatcc catctttctt cgcatcagca cggagaagtg cattgccctt gctggcgaca
      481 aaagcgtgat aggtgataag tgataggtga taggtgtcta accccggctg cgccgggaat
      541 tcatatcact tatcactcgt cacttgtcgc gctttttcag atgctggcaa aggcgcgggg
      601 cttggagtag attttccgca tgcaagaatg aggagctgaa aatggcgaaa aacgcgatgg
      661 tttcggagga gatggcgaaa aaattcgcca cggagaagga gacgccctac acgcgctggg
      721 tgaaagcaga ggggctcgac atcctcccct cgcactatgt ccccaacttg cacaccgtcg
      781 agctcaagcc gtgggcgcgc cgcggcgggc gcgggatgta tttcaaccac gaggcgtcgc
      841 gcacctcgaa cgattgctac gtgtgcgaga tcccggcggg aggcaagctc gcgccgcagc
      901 ggcagctgtt cgaggaaatg gtcatgatcc tcagcgggcg cggctcgacc gccgtgtgga
      961 acgacaccgg gggccgcatc accttcgaat ggaaggcggg cgcgctgttc gcgattccgg
     1021 tcaacgcgtg gcaccagcac ttcaacggct ccgggcagga gccggcgcgc tacgtcgcgg
     1081 tcaccaacgc gccgatcgtg atcaatctct acgacgacgt cgacttcgtg ttcaacacga
     1141 agtacgactt caggaaccgt ttcgcgggcg agccggacta cttcgcgccc aaaaacgagc
     1201 agaagggttt cctgctcgac accaatttcg tcgccgacgc cgtgaacctg ccgctgatcg
     1261 cggccaagga gcgcggcgcg ggcggagggc acatccgctt caacatggcc aagggctcga
     1321 tgaatagcca catctcgcag tttcccgtcg gcacctacaa gaaggcgcac gctcacggcc
     1381 ccggcgcgca cgtcatcatc ctctccggcg aaggctatac cttgatgtgg cccgagggcg
     1441 aggagccgcg ccgctatgaa tggaacgtcg gcgcgatgat cgtgccgccg accatgtggt
     1501 tccaccagca cttcaacacc ggcaccacgc cggcgcgcta tctcgccttc aagtacgaag
     1561 gcaccgcgat ccgcaacgcg cagggggtgc ccaaggcctg gatcagccgg cggctgggcg
     1621 gcgaccagat cgattacgcg gacgaaagcc cggtcgtgcg ccgctggttc gccgaggcgc
     1681 tggcgaagca cgacatcaat tcgcggatgg acgaagccta ccaggccgag ctcgcgagcc
     1741 ttcagcaagg cacattggtc gcggcgggcg gcgacgactg aaaggcaagg cggaagtcga
     1801 aaggcgaaag gaggaaggat gaggaaagcc gagccggctc aatctgctgc ggagcagaaa
     1861 cactttcgcc tctcgccttt cgcctttcgc cttgcggcgg cgttgctgct cgcagcaccc
     1921 gccgccgcgc cggcgtccgc gcagccgagc gtcgccgagg tcgccggcct gcagggcccc
     1981 gaccgcatgc agcggctggt tgaaggcgcg aaaaaggaag gcacgatcac catctacacc
     2041 tccgccacgg tcgaggacat gaaagtcctg tcggccgcgt ttgaaaaaaa acacggcatc
     2101 aaggtgaacg tctggcgcgc gagctccgag aacatcgtgc ggcgcggcat ctccgagatg
     2161 cgcggcgggc gcaacgaggc ggacatcttc gagaccaacg ggcccgacat ggagtcgctg
     2221 caccgcgagc agctgttcca ggaagtgaag tcgccccatc tcgccgacct catcccgggg
     2281 gcggtcgcgc cccaccgcga atgggtcggc gcgcggatca acatctacgt catgacctac
     2341 aacacccgcc tcatccggaa agacgagctg ccgaggagct tcgaggacct ggcgaacccc
     2401 cgctggaagg gcatgctggg catcgaggcg agcgatccgg cctggttctc gacggtggtg
     2461 agcgatctcg gggaagcgaa ggggctgaag ctgttccggg ccatcgtggc ggcgaacggc
     2521 gtctcggtac gcaagggcca cagcctgctc accaatctcg tggcatcggg cgaagtgccg
     2581 ctcgcgctga cgacctacca ctaccgggcc gagcagctca agaaaaaggg cgcgcccatc
     2641 gactggttca tcctgccgcc cgcggtcgcc cgcttccagg gcatggggct cgcgcgccgc
     2701 gccccccatc cccacgccgc agtgctattc ttcgatttca tgctgacgga cgcgcaggag
     2761 ctgctcgcgc agcgcaattt cacggtgacg agccggaaga tcgactcgcc ggcgcacaag
     2821 atgcccctca agttcgtgga tcccaggctg gtgctcgacg aaagcgaaaa atggggtaag
     2881 ctgtacgagg aaatcgtcgt caagcagtcc cggtaaccgc cgcaaggagg cttcatgggc
     2941 aaggcatcgc actaccacga caagaacgac cggatattcg tccgggcgat tcaggggcat
     3001 tacaacctga aggaagagct cgcccggctg cgctcgatgc cgcgggtcgt caggggcaag
     3061 gagctgaaat tccaggacgg gccgcagtcc tacagcaagc atttcgtgga accggtggac
     3121 ggcatcacgc agaccctcca catccacctc gaggagtacg cgccgggcgg gcggacgcag
     3181 aagcacggcc acgtgaacga ggcggcgttc tacatcctcg acgggcgcgg ctacgagatc
     3241 cacgacgggg tgcgctacga ctggcaggcg ggtgatatcg ccatcgtgca caacaactgc
     3301 gtgcaccagc atttcaacgg cgatcccgac aagccggcgc gtttgctggt gctcaagtcc
     3361 aagcccatgt tcctgtggat gaacatgctg ttccagaaaa cggtgatccc gaagccgaag
     3421 gagccggcgc cgggagccga gggatacgag ccgaggaacg atgaagtgga ctacaaccat
     3481 tgaccgctat cctcctcccc cttgggggag gaccaggcgg ggggttaatt gaaggagatc
     3541 aaagatggcc tggggtgaat cgaaggcgtg gatgcgcggt acggcgagcg ggaagctcta
     3601 ccagaacctg ctggacgatg cgaagggcgc tccggcgcgc aacgccaagc gcaagaagat
     3661 cgtgcatccc gaggaaatgc cttgggagat gtcgcggcag gggctgctca agcacctgct
     3721 caacgagcag atgaacacgc gcatggagac ggtggacgcc tacatgcaga tcattccgcc
     3781 cgggagccgc tccggcaggc accggcacct ggcggaggaa tgcctgttcg ttgtggaggg
     3841 gcggggctac gacctgcacc aggattgcga cgtcgagatc accgacacct atcactggaa
     3901 gccgcaggac gagatcaagc gctacgaatg ggaagcgggc gacgtgatct acatcccgcc
     3961 caacaccatc caccagcact tcaacgcgga tcccgaccgt cccgtgcgac tgatctcggc
     4021 gatcaaccgc atctacaaga actgcgggct gaacgatctc gagcagctgg aggacgcgcc
     4081 ggagtacgac cccaaggtcg tgctgaccgc cgaggtgatc aagcgctacc tcgcggcgaa
     4141 ggtcaaggaa ccagcctgaa gcggccaggc gtcagcatgc agacggggtg gtagcgcgct
     4201 tcaggcttac tggcgctcgc ctcgactccg gtgtcgtagc caaataaggg cgccgcggct
     4261 atcaccgcag cgcctgtttt tttcaccgtc ttcatcagcg gcgccctcac atgtccaaac
     4321 cctcgacaaa gccgaaagaa aatccggccc cttccgccgc cgcgaaccgg cgctgggctt
     4381 ccgacgtcat cgtcgacatg ctgcaccgct acgagctgcc gtacgcggcg ctcaaccccg
     4441 gcgcgagtta ccgggggctg cacgattcgc tcgtcaacta cggcgggaac aagccgtaca
     4501 tgatgctgtg ccagcacgag gagacggcgg tgcagatcgc ccacggctac gccaaggcga
     4561 gcggcaggcc gatggtcgcg atcctgcaca acctcgtcgg cctgctccac gccaacctgg
     4621 caatctacta cgcctacatc gaccgcgcgc cgatcttcat catcggcgcg accggcccga
     4681 tggacgaaac caagcggcgt ccccgcatcg actggacgca caccgcccag gtgcagggcg
     4741 aggccgtgcg cgcctacacc aagtgggact accagccgac cgtggtggac ggcgtgcccg
     4801 agtcgtttgc ccgcgcctac agcgtgatga tgaccgagcc gcaggggccg gtatacctgt
     4861 gctacgacgc ctggctgcag gagcaaccgc tcgaccacga cgtgcccctg ccgccgccga
     4921 cctccatgcc ggttccctcg ccgttcaccg cggacccgac cgcgctcgcg cgcgccgcag
     4981 aaatgatttg cgaagcgcag cggccggtca tcctcgccga gttcgtgggg cgcgatccga
     5041 agggtttcga tcagctggta gccctggcgg aaacgctggg tgcaccggtg tacgacgtca
     5101 acagccgcct caacttcccc ggctgccatc cgttgaactt gagcatggtg aaggacgtgt
     5161 tcaaggacgc ggacctgatc ctgtgcctgg acgtgcgcga ctgggaacgg gcgaccacgg
     5221 aactggtgag ccagacccgc acgttgacca gcatcgagcc gccggattgc gtctggatcg
     5281 acatcggctt cggcgacctg gagctgtcga gctgggccat ggactaccag cgcctgaagc
     5341 acgcgcaact gcgcatcctc gcggatactg cgctggcgat cccggcgctg accgacttgc
     5401 tgcgcaagag aatcgccgca agcccggggc tcaagaaaac catcgacgag cgggcccgcg
     5461 ggaccgccga acggcaccgc gaggcgcggg cgaagtgggc gcagcaggcg cgcgaggact
     5521 gggacgcgac cccgatcccc ctgccgcgcc tcgccagcga ggtctgggac gtgatccgcc
     5581 acgaggactg ggtcctcacc gccggcacgc tcgaggactg gacgcgcaag ctgtgggatt
     5641 tcgaccggcc ctaccggcac cccggcaagt cgctcggcac ggcgacgcag atcggcctat
     5701 cgctcggcgt cgcgctcgcg caccgcgatg ccggcaggct cgtcgtcgac ctccaacccg
     5761 acggggacct gatgttcgac gccggtgcac tgtgggtcgc ggtcaagcac aagatcccga
     5821 tgctgatcgt gatgtacaac aaccgcgctt actacaacga ctgggagcac cagatccgca
     5881 tggcgcggca gcgcggcacg cccatcgagc gcgctcacat cggcatggac ctggaggagc
     5941 ccacggtgga tttcgccggc ctggccaggt ccatgggctg gtacgccgaa gggccgatcg
     6001 agcgcggcaa ggacgtgggg cccgcgctca agcgcgcgat cgcgcgggtg aaggcggggc
     6061 agcccgcgct gctcgatacc atcacccaga agcggtgatc gtgcgctacg cttgaaggat
     6121 gaaggcggaa ggatgaagga tgacctgacg gttctgtcct ggcctttcac tgaccgtccg
     6181 gccagatgca gaaggcaaag gtagaccccg cccgtacgtt agtgaaaggg tgggacaaag
     6241 cagataatca cgcgaggcgt gagggtcatc cttccacgtt catccttcat ccttaatatt
     6301 cgcgatggtg ctcggagcat gataggtcga tgtcatggta acagtggctc aaaacgatta
     6361 cttgacgcgc accggtccgg gcacgccgat ggggcagctg ttccggcgct actggttacc
     6421 ggtgctgctc gagcaggagc tgcccgggct ggattgcccg ccagtgcggg tcaagctgct
     6481 gggcgagcgc ctgctcgcct tccgcgacac gcaaggcagg ctgggactga tctcggagtt
     6541 ctgcgctcac cgcggcgtga gcctgtggtt cgggcgcaac gaggaaagcg ggatccgttg
     6601 tccctatcac ggatggaaat acgacgtgag cgggcaatgt ctcgacgtgc cctccgagcc
     6661 gccggagaac ggcttcgcct ccaggatcaa gctcacgagc tatcccctga tcgagcgcgg
     6721 cggcgtcatc tgggcctatc tcggcccgcc ggaatcgaag ccgcacgagc ccgcgtacga
     6781 gtggacgctg gtgccgccgg cccaccgcta tgtctccaag cgcctgcagg aatgcaatta
     6841 cctccaggcg atggagggcg ctctcgattc gatccactcg acctttctgc accggcattc
     6901 cgtcgccgac gatccgctgc tcaagcgcga cccgcaaagc ctggagttga tccggggtga
     6961 cgcaaagccg gcgttcgtgc cgacggtctc gccggccggc ctgtacatcg caacccgccg
     7021 caacgccggc gaggaccgct actactggcg cgtcacgcac tggctgatgc cgtgcttcag
     7081 cttcttcccg ccgtacggcg acaatccatt cggcggccac gcgttcgttc cgatcgacga
     7141 cgagaactgc tggaccttca gtatcgacta tcacccgcgc cgggcgctca gccggaccga
     7201 gcacgacgcc atgcacgccg gaaagggcat acacgtgcgc ctgatcgaag gtacgttcac
     7261 cccggtcgcg aacaagcgca acgactaccg gatcgaccgc gcggggcaga agacgaaaaa
     7321 aacgttctcc ggcgtgttcg acgtcggcgt gcaagacgcc gcggtgcagg agagcatggg
     7381 gccgatacag gaccgcgcgc gggaacacct cgtttcatcg gacaacggga tcatcatgac
     7441 gcgccgccgg ctgatggatg cggcggatgc ggtcggccgc gggatcgcgc cgcccggtct
     7501 cgaccccgcg gagcagcgcg agcgcgccct gtccatggtg acgcctcgcg agctgccgct
     7561 cctcgacgcc attgcccgat acagcgatgg gcaacgggcc tcgtcgccgg gagatcggga
     7621 ggtcgagaga ccgtgagatt gaaaatacgg gagatcgtga gatcgggagc tcgtgagagg
     7681 gattttttcc atttcccgct ctcacgctcc cccgccctca cggtatttgt cgctctcccg
     7741 ctctcacgcc cttccgccct cacggacctc caagcgcaat aaggacgtga acgcatgaac
     7801 tatgaagcat cggacaggat ccagaatgta ggtgtgattg gcgccggccg catgggacag
     7861 ccgatcatcg gtcacctcgc gcgcaagggc ttctccgtgc aggtccacga catcgatccc
     7921 ggcaagcaag ccgcgatcga agcgcgcggc ggacgctggg ttgccgacgc cgccgcgctc
     7981 gcgcgccaga gcgacgcgat cctgatctgc gtcggctacg accacgagct gcgcgagctg
     8041 ttgtccgagg acggcatcct caaccacgcc cgccgcggcg cgatcatcgc cgtgctgagc
     8101 acagtgcatc cggaaacggt gcgcgagctc gccgagcgcg gccgcgcgcg cggcctgcac
     8161 gtcgtggacg ccacgatgtg ccggggcgca tgggcggcag acgagggcac cctgctctcg
     8221 ttcgtcgccg gaactgccga gatcgtcgcc cggctgcggc cggtgctcgc ggcgttctcc
     8281 agcgacatcg tccccaccgg ggacatcggc tcggcgcagg tcgccaaggc cgtcaacaac
     8341 atggtcatgt gggcctgcct ggtcgcgaac cacgaggggc ttgcgctcgc gcaccgctac
     8401 ggcctggaca tcgagacggt gcgcaaggcg ctcctcacga cccacgcagc gaacgcagcg
     8461 ctggagcact ggggcaggca gagcatggct tgggccgacg atgacatgga aatcgttgcc
     8521 gccatggcga aggagaaagg cctggcgctg aggcaggcga aggccgtgca ggagatctgc
     8581 cgcacgctca agccccggcg ctaccagctc gataaattcg gcctgtgaaa ggagataggg
     8641 tcgggccgac cccctttcac cgcggcccgc ccttggagta gactaaaaag ggcattctgc
     8701 tggatcgtgt cgcaaggaga gagccatgac tgcgtcattc gacgagaaac gcgaggggct
     8761 cgagcacgca ttgcgccata tcggtgagtc agtcaccgaa gctgatgtgc aggccttcat
     8821 tcatcaggag actcgcgcgc gggaagagcc cggtgctccg gtcacgcgcc gccggttcct
     8881 gtccggggtc ggcatggccg ccgcaacggc cggcgtcgcg gcgttatccg cgcggcctgc
     8941 ggacgcgaaa gctccaccgg gcgccatcga atacccggtg caggccgatt ccacgcggga
     9001 gcagggccgc gtgatgggcg aagacggcgg ctacggcagc cggtcgcaat tcgagaccga
     9061 ggtgagggcc tggcagccga cgcggaccgc gtccttcacg ccgatgcaaa aagggcacgg
     9121 catcgtcacg ccctccggcc tgcattacga gcgccatcac ggcggcattc ccaatatcga
     9181 tccgcgcaag cattcgctga tcatccacgg actggtcgag cggccggtga agttttccgt
     9241 cgccgacctg atgcgatttc cctcggtgtc ccggatctac ttcatggaat gctccggcac
     9301 gagcggcagc gagctcatga aggcgacgat gccgacggtc cagcgcacgc acggcctcgt
     9361 gtcgacgtcg gaatggacgg gcgtgccgct ctcgacgctg ttcaagcagg tagggctcag
     9421 aaacaacggc gcatgggtgc ttgccgaggg cgctgacgcc gcggtcatga cgcggagcgt
     9481 cccgctcgac aagtgcctgg ccgacgctct catcgtgtac gcgcagaacg gcgaagcgat
     9541 ccgccccgaa cagggttatc cgatccggct cttcctgccg ggatgggaag gcaacatctc
     9601 gatcaagtgg ctgcggcggc tcgaggtcgg cgataagccg taccagacgc gcgaggaaac
     9661 gtccaagtac tccgacatca tcaccaagac gggcaaggcc cggcagttca cgttcacgat
     9721 ggaagccaag tcggtgatca cgtttccctc cggcgagctg aagcttcccg gccccggttt
     9781 ctacgagatc acggggcttg cgtggaccgg acgcggaaag atcgcgcgtg tcgaagtgac
     9841 caccgatggc ggcaagacgt ggcatctcgc gtcgctgcag gatccgatcc tgccgatcgc
     9901 gcagacgcgt ttccgttatc cgtgggtgtg ggatggctcg ccggcgatca tcgcgagccg
     9961 cgccaccgac gagaccggct acagccagcc ctcgaaggag cagctgatcg ccgagcgcgg
    10021 gccgctggag gtcgggcttt tctaccacat gaacgcggtc cagcactgga gcatcgaccg
    10081 tgaaggcagc gtcaagaact ggcatccgct gcagaagggg tagcactatg ggaaggttcc
    10141 tcgttgccgg caccgctatt gctcttgcgc tcgctgcaat gggcgccggc tatccggaga
    10201 agttcgactt cggtgctcca gccacggacg ccgagatcaa agtcgtcgcc attgcgattc
    10261 cccccgacgg caagtggctc ccgccgggaa acggcgactg ggcagctggc aagaaagtct
    10321 acgacattca gtgcgcgggc tgccacggtt cggatctgct cggcgtggcc ggcatcaagg
    10381 acatgccggc cggcgcccag ctgcgcctga tcggcggccg cggcacgttg acgtcgccga
    10441 agccggtgct gacggtcgag agttactggc cgtacgcgac gacgctgttc gactacatcc
    10501 ggcgcgcgat gccgtttttc gcgccaggct cgttgtcgaa cgacgatgtc tatgccgtgt
    10561 cggcctacat cctcggcgag gcgaagatca tcgacaaatc ggcggtaatg aacggggagt
    10621 ccttgccgaa ggtaaagatg ccgaaccgcg acggcttcat tcccgatccg aggccggagc
    10681 ggtttgggac gcggtaatcc gtcttcgaca agaatccaag agcgattcaa cagatgcgtc
    10741 ctgacgctgg acgcaggcgt gcattccatg tgctgctcgc ggttgcgagc ctgcttgtcg
    10801 cgctcacgtc cgccgcggtg caggagatct gccgcacgct caagccgaag cgattcaagc
    10861 tcgaccagta cggtcgctag aacaacgcga agcgtgacgg ctgacgactg acgacaggag
    10921 cagccggtga atcgcaggca cttccgactt tcgaattccg actcccgact ttcgctggcg
    10981 ctggcggccg catgcctgct cctggctccg ccagcgtcag cgcaggacgt gtggccgtcg
    11041 cgtccggttc gactcgtcgt tccctcctcc cccggcggcg gcaccgatac ttacgcccgc
    11101 ctgcttgcgc agggcctcgg cgacgcgctg aagcagcaat tcgtggtgga caaccggccc
    11161 ggcgggggcg gaaacatcgg cgccgaaatc gcggcccggg cagcgcctga cggatatacg
    11221 ttcctcgtct cctcgagtcc ggcgctcgtc gtcaatccga gcctgttcag gagcctgccg
    11281 tacagcgcgg agcgcgactt cacgccggtc gcgcgcggtg tggtcagccc gctcgtgttc
    11341 gccagccatc cctcggtgcc cgtgaaatcg ctggctgccc tggtcgcgct ggccagacgc
    11401 gagtccggca gcctcagcta cggttccccg ggagtgggca gctcgtccaa tctggccgtg
    11461 aagatggtcg aggaagccgg gaaggtgcgg ttcctgcacg tgccctacaa gggggcggcc
    11521 caggcggtgc tggggctgct ccgcggcgag gtcgcgttca tggtcaccga catcagcacc
    11581 gcgctgccgc acattcattc caggaaagtg gtgccgctcg ccgcgtcgca tcgctccccc
    11641 cacattcccg gcacgcccac ttacgccgag gccggcttcc cgagcgtcga cgcctatccc
    11701 tcgtacagcg tcgtcgcgcc aaccggaacg ccgcccgcca tcgtccagcg catgagcgcg
    11761 gagatcgttg gattgatgaa atcccccgcg taccgggaga ggctggacgc gcgcgcgttg
    11821 atcccggtct tcgacacgcc ggacgagttc gccgccgtcc tgaaaagaga gcgggcgcgc
    11881 tacgccgagg tcatccggcg caacaacatc acggcggagt agccgcctcg aggatgaagt
    11941 cggaagtcga aagtcgaaag gcggaagtaa cggcacaaat caagccggcc cggccgtcgg
    12001 ccgatcatta ctttcgactt tcgcctttcg acttccgcct tgcgatagcg gcgctgctgg
    12061 cgctctttgc cgccgcgccg gcgctagcgc agagtttggc ggagctcgcg gcctactccg
    12121 gcgcggaccg cgagcagcgc ctcgctgaag gcgcgcggcg cgaaggcgtt ctcaatctct
    12181 acacctcgct caccgtcgac gacatggcgg tgctgaacgc cgccttcgag aaaacgaccg
    12241 gcgtcaaggt gcgcatgtgg cgcgcctcct ccgagaagat cgtgcagcgc gcgctcgccg
    12301 aggccagggc gggccgtttc gacgtggacg tgatcgaatc gaacgccccg tcactggaaa
    12361 tactgcaccg ggaagggctg ctgcaagcgg tgcgctcgcc ccatcacgcg gacctgatac
    12421 cggccgcggt gccggcccac cgcgagtggg tcggggcgcg gctcaacatt ttcgtccagg
    12481 cctacaacac cagactggtc cggaaagagg aactcccctc ctcctacagc gacctgttgc
    12541 acccgcgctg gaagggaagg ctcggcatcg aggcctcgga tgaagattgg tttgcagcgg
    12601 tcgtgcaaag catgggggaa gaaaggggat tgaagttgtt tcgcgagctg gcggcggcca
    12661 acgggttgtc ggtgcgcagg ggtcacactt tgctcacgaa cctggtggtc gcgggcgaag
    12721 tgccgctcgc gctcacggtg tacaacttca ccgccgagca ggtgaagcag aagggggcgc
    12781 cgctcgactg gttcgttctc ccgcccgcca tcgcgcgggc gaacggagtc gccgtggcca
    12841 ggcgcgcccc gcatccccac gccgccctgc tctactacga attcatgatc ggcgacgaag
    12901 gccagagcat cttgttgaag cgcgatttcg ttccggcaag ccggaagatg gacaccgagc
    12961 tcaatcgggg cccgctgacg atcatcgacc cggcgctgat cgtggaccag ggcgagcgct
    13021 gggcgaagct ctacgacgag atcatcgtca agcaaggacg gtgacaagtg atgggtgacg
    13081 agtgacgaga aaaccctttc gcctttcgcc tttcgccttt cgccttgttg cggcgggact
    13141 ggcggtgctc gcgctcgctc cagcgaccgc gagcgcgcag gccaccgccc agtcgacgct
    13201 cgcaacctac gccggcgccg atcgcttcga gcgcatcgtc gcggcggcga agaaggaaag
    13261 ctcgctgatg ctttatacga ccttcgcggc gagcaacatg gaagccgtcg ccaccgactt
    13321 cgagcgccgt tatggcgtca aggtcaattc ctggcgctcg ggcacgctgc gggtgctgca
    13381 gcggacgctg gcggaagcga aggcgaaccg cttcgaggtg gacgcggtta tcttcggttc
    13441 cccggaaatg gaagccctct accgcgaaca actgctgcag gaagtccgtt cgccccatca
    13501 cgggaacctg gtcgatggcg ccttgccggc gcaccgcgcg tggtccgccg tgtacctcaa
    13561 cctttttgcg cacgcgtaca acaccgacaa ggtcaggaaa gaggagcttc ccaggaccta
    13621 cgccgacctg ctccacccgc gatggaaggg gcgtctcggc gtggaatccc aggaccagga
    13681 atggttcctc gccgtggtga aggacatggg cgaggccagg ggcctcaagt tcttccagga
    13741 cctggtggcg ggcaatggcc tgtcggtgcg taccgggcat tccctgctca acaatctggt
    13801 ggtgtccggc gaagtgccac tggcgctcac cgtgcacagc tacatgcccg aacagatgaa
    13861 gcgcaagggc gcgccgatcg actggttcgt gatggaaccc gccgtcgtgc ggccgaacgc
    13921 ggtcggcgtc atgcggcggg cgccgcaccc gaatgcggct ctgctgtttt acgaatacat
    13981 gttaagcgac ggccagaaag tgctcgccga cctggcctac atcccggcca cgcgccaggg
    14041 cgactcgcca ttgaggaatc tgcgcgtgcg cgtggtcgac cctgtcgagg tcctggacga
    14101 gaacgacaag tggtcgaagt tgtatgaaga tacgctgcgc ggcgcccgct gaaacaaatc
    14161 catcactcat cactcatcac tatgattatt gacctggatt cgcacctgcg cgaagggtac
    14221 tttctcgacg aggtttaccg gctcgacggg ccgctcgccc ggttcacgcc gcggaaggtg
    14281 ggcgacggcc gctcgcacgc gacgcagttc attcactcgc tggagcccgg cgatgcgcgc
    14341 gcactcgccg cgttcaagca tccgtacatc tacgatccga agaacgaccc ggggctggcc
    14401 gagcgccaga ggggtggtta cgacatggcg cgccggctcg cggacttgcg caaggaaggc
    14461 atagacaagc agatcatctt tccgaccggc atcgacatcg cgaccaagaa cgccggcgag
    14521 ctgggcgcgg ccctcgcccg ctgctacaac gactgggtcg gcaacctcgt aaggggctac
    14581 gaggacgtct tcatgccgtg cacggtggct cccgcaggct gccccgagca gatggcggcg
    14641 gaattgcgcc gttgcgtgca ggagaaggaa ttcaaggccg gtcacctggt gtgctattgc
    14701 ggcccgcgca atctcgacga cgcgtcattc cacggctact acgcagccgc gcaggagctc
    14761 gacgtcccgc tgctgtgcca tcccaacagc gtcggcgaat tgaccgaccg cttcaacaat
    14821 ttcttcaagc tgcacatcgc gggtcgcccc aacaactgca ccacggcgtt gatcgcgctg
    14881 gtggcgggcg gcgtgttcga gaagttcccg aaattgcgtg tcgccttctt cgaatgcagc
    14941 gcggagtgga tgctctactg gatgcaccgc atggacgacg actgggaatg ggcgagccgc
    15001 taccccgagc agaccggcgg cagtgcgctc aagatggcgc cctcggagta catcaagcgc
    15061 aattgctacg tcacctgcga ggccgacgag cgttacctgg gcaacgcggc ggccgagctg
    15121 ggcgcggacc acatctgcat ggcgacggat tacccgcact tcgactcgaa gttcccggag
    15181 accgtttccg gcatccggca acgcggcgat ctgaccgcgc cgcagaagga aatgatcctc
    15241 ggcggcaccg ccgcgaagct gctcaggttg taacgggatg aggcgtgact ggcggcctgc
    15301 gatcaaaaca gggctccggc cgccggttgg caaccagatc cgccttaccc cgtcactgcg
    15361 ccaccttcgt cctcgttcag gaatagaatg gcggcttcgc ctgtttaccg gtcgcccgaa
    15421 cgcctcactt caccctggga ggagacacat gaagccctgc ttgatcaccc tgttcgccgg
    15481 tgtattcgcc ctgtcagcca gcgccgccga cgcgcaggag aagcccatgc gggtgcgtgg
    15541 aaccatcgtt gccgtcgagg gcaacgtcct cgccctgaaa tcgcgtgacg gcaaggacct
    15601 gcaactccag ctgacggaca agacagccgt cgccgcggcc agggcgatca agctggccga
    15661 tctcaagaag ggcgactacg tcggctccac aacgatgaag aatgcccagg gcgtcctggt
    15721 cgcgcacgaa gtgcaccgga tcccgcgcac cgtcaaggag ggccacgggc cgtgggacct
    15781 gctccccaat tccatgatga ccaacgccaa cgtggcctcc gtggtaatgg tcgctgacgg
    15841 cgagcagttg acgcttgagt attcgggcgg atcgcaaacg atcctggtgc ccgccggaac
    15901 gccgatcgtc gagaccgcgc cggccgaccg ctcgttcctg aagcccgggg agtacgtttt
    15961 cgtcgcggcc caggccggcg cggacggcaa gatcacggcg ttacgaatcc aggtaagcca
    16021 cggcggcgtc aggccaccgc aatagcggca gccaaggcgg aaggatgacc tggcaccgcc
    16081 tccgtcagaa ggatgaaggt tgatctggtt cctgcttccg cgctgcggtg gtcatccttc
    16141 acccctcatc cctcatcctt cgtcccccca gagtgaaatc catgaaatta ctccacccct
    16201 gtctcctgtt cgttgtgctg gccctgatgg cgggcgctgt tgaagcgcag gctcccacgc
    16261 gggtgcgcgg gaccgtcagc gctttcgccg gcaacctgct ctcggtaaag tcccaggacg
    16321 gtagaaacac ggagattcag ctcgccgaca attccgtcat cgtcttcacc cagccgatcg
    16381 cgctcgcgga catcaagccg ggcgacttcc tcggggtcac ttcgatgaag cgcgacgacg
    16441 gaacgctcac tgcttacgaa gtccgccgtt ttccaaaacc gctcaacccg ggacaccggc
    16501 ccttcgacgg caggaacgac cagaccatga cgaatgccac ggtggctgcg acggtccagt
    16561 ccgccagcgg ccgcgagctc accctgacct atgaaggggg tgcgcagaag atcatggtgc
    16621 cggaaagcgc gtcgatctcg atgctcgtcc cgggggaacg ctcgcacctc gttcccggcg
    16681 cggccgtgaa cctgaccgcc acgcccggcg acgacggcaa gctgaccgcg ctgcgcatcc
    16741 aggtcagccc gccgcggtga ccgttcggtc ccggcgggag ggatgagggc ggaggaataa
    16801 ccttgaaccc gttttcagct tcgcggtttt ttcctcattc ttcatccctc atccctcatc
    16861 cctaggttca ccccgtggac tgccggcgct ggtaaaccgc cacgcttcgc agtaaagtta
    16921 cccgcctgat ccgacctcgc gcgtcgcggt tgccggcgcc gtttcattca ttcacgacca
    16981 tccaaggggg ctcaccgtga tttacgaatt ccggacctac gatctcaagc cgcattcgct
    17041 gccggaggtc gagaagcgct tcggcgaggc ctacgagacc cgcaagaagt tttcgcagct
    17101 tgccgcgttc tggcacaccg agatcgggcc tttgaaccag gtcatccacg tctggccgta
    17161 ccaggacctc gcggagcgcg ccaggatccg cgacgccgcg gtcaaggaaa gcggcggcaa
    17221 gtggccgccg aagaccgccg agttcatggt gagccagcgc tcggacatct tcatcccgtt
    17281 cgacttctcg cccgagatca agcccggcaa gcaggggccg ttcttcgaga tgcgcaccta
    17341 cacctacgcg accggccagc tcggcaatct gaccaaggcg tgggcacacg ccatcccttt
    17401 ccgcatgaag cacgggccgg tgaccgccat cctctactcc gagctgggcg ggctcaacaa
    17461 gttcgtccac atctggccgt acaagacgct cgacgagcgc tgggcgatgc gaaacaagat
    17521 gcgcgagacc ggcgactggc cgccctcggt cgcggccaag aaggcggggc tgcccgagta
    17581 ccacctggtg cagcaggaga acaagatcct catgccctcc gctttttccc cccttcagta
    17641 agggaaaaag cgggaaggat gaaggcggaa ggatgaaggc tgacctgatt ctgacttccg
    17701 caccgtcggg gatcatcctt catccttcat ccctcatcct tcgataggag aaccgtatgt
    17761 acatgatgga actcgccgac cgcgggatgc aggacgagac ccgcatgatg cgcgacgtca
    17821 cccgcaagtt cgtgaacgag cacgtcattc ccttcgtgcg ccagaactgg cagcaggaat
    17881 ggagcatgaa cccggatggg cggctgccgc ggaaaattct cgaagtggcc gacgccatcg
    17941 gcatccgcac gctcggcgtt cccgaggagt tcggcggcac gccgctcgac cccaagaccg
    18001 aggtccagac cttcgcggtg atctcggagg agatctcgcg cggcgattgc gggctttccg
    18061 acaagatggt gcagatctgg aaggtgtcgg tgctgctcag gaacctcgcg ccgcgccacc
    18121 tgcaggaact ctggttccca cgcctcgtca aggacccgac cttcctgctc gcgcactgcc
    18181 tgaccgagcc gcgcggcgca tccgaccgct ggctgcccta caacgtgccg gaagcggcga
    18241 tgcagaccaa ggcggtgctg aagggcgacc gctgggtggt caacgggcgc aagcagttca
    18301 tcagcaacgg ctacgacgcc aagctctacg tcgtctacgc caacaccaat ccgaaggtcg
    18361 gcatgctgca gggcacttcg agcttcctcg tgccgcgcga caccaagggc ctcaccgtgc
    18421 aacgctgcaa cgagacgctc ggcggacgct tcatgaacaa cggcgaactc gtgttcgagg
    18481 acatggagct gccgaaggat cacctgctgg tcgagggcaa cgcgctgtcg cgggcgggca
    18541 tctacttccg ccccggcaag atcatccagg gctcgaagaa cctgggcgtc ggcgtcaggg
    18601 cgttcgagga aactgccagg tacgtgcagg agtacgtgca gggcgggagg atcctcatca
    18661 agcaccaggc gaccgccatc cggctcgccg agatggcgac caagatctgc gcgacgcgcg
    18721 ggctgctgcg cgaggccgcg gccgcggtgg acgcgaacgc tccggacgcc gacgtgctgt
    18781 gcaacatggt gaagctctac gcttccgagg cggtgctgga agtgtgcaag cacgccatgg
    18841 agctgcatgg cggcaacggc accatgctcg acttcggcat cgagaagctc taccgcgacg
    18901 cctccatgtt cctgcacatg gacggcaccg tggacgtcac caagttcaag atcgtgaagg
    18961 cgctgttccc ggagactgcc gggaagtacg ccgggcccga aacctgaaaa ggcgtgagtc
    19021 gtgagtggtg agtgacaaga aagggcggcc cagccgccct tcttgtttca ggctctgggg
    19081 aatgtcccat cactcatcac ccatcactca ttactggggt ttgatgcccg cctgcttgac
    19141 caccttcgcc cactgcgcga tctcggcacg gatcatcgcg gcgaactgcg cggaaggatt
    19201 cccgaccggc tcgagcccca tggcggcgaa ctgctggcgc acgtcggccg actgcagagt
    19261 cttcacgatt tccgcgttga gcttgtcaac gacgggcctg ggcgtgcgcc ccgtcaccag
    19321 gatgccgtac cagaccgtca cgtcgtaacc gggcacaccg gcttccgcga tggtgggaac
    19381 gccgggcgcg gcaggccagc gcgcgggggt cgtcacgccc acggcgcgca ggcggccggc
    19441 ctgaacgtga ggcaacgcct gcggcagccc gctgaacacc atctggacgc gccccgcgat
    19501 gatgtcggga atactgacgg cccccttgaa cggtacatgc accatctgga tgccggccat
    19561 gaggctgaac agctcggcgg ccaggtgctg ggcgctgccg atgccgcctg acgcgtagtt
    19621 gagcttgccg ggattcgcct tcgcaaggcc gatcaactcc ttgaccgact tcgcctccaa
    19681 ccccggatac aggacgagaa tatagggcgc agccgacatc tgcgtgatcg gcgcgaaatc
    19741 gcggatcggg tcgaacggca gcttgccggc gatgctcggc tggatcgcga agctcgggct
    19801 ggtggcgagc agcgtgtaac cgtccggatt cgccttggcc gccatctcga aggcgaggag
    19861 gccgcccgcg cccgcgcggt tgtcgatcac gacttgctgg ttccacgtct cggagagctt
    19921 ctttccgata atgcgcgccg cgacgtcggt ggcgccgccc gggctgaacc cgaccagcat
    19981 cctgatcggc tttaccggaa aactcggttc tgcggcatgc accgcactag aaatcaccgc
    20041 aaagccgagg attagcactc cgcatattaa cgacatcagc gcggttcccg cactgcgggg
    20101 ggcatccttc atccttccgc cttcatcctt aaatagtttt cgtcactgcg tggcgaaaac
    20161 gatgcacgtc gggcttcccg tctcgatgat ctgcccggtg ggcgtgagct tgccggtttc
    20221 ctgatcgatt cgaaactcga cgatggtgtg actgttttcg ttcgccgcgt agagacagct
    20281 cgcgcccgga tcgagcccga aaaagcgcgg tttcctgccc tggaccgatt cccagccgac
    20341 gggctcgatc aagccggtgg actggctcac ggaatagatc acgatgctgt catggccgcg
    20401 gttcgaagca tagaggaatt tgcccgaggg caggatggcg atttcggccg ccatgttgtc
    20461 cccgacgtag gtggtcggcg tcgtcgggac ccgctggaag ggtttcaacg cgccgcgccc
    20521 ggtgtcccag tgataggcgt tgacggtcga gtcctgctcg ttgacgacat aggcgtaggg
    20581 gcacgtggga tggaacgtga tgtgacgggg gatcgcgcca tagcgggact tcacgaacgg
    20641 cgggtcgcag ggcgccagct tgccgcgcgc ggcatcgaac cggtagacca gcactttatc
    20701 cagccccttg tcgggcacca cgacgtagcg gccggtcagg tcgaaagtgg cctggtgggg
    20761 atgcgacccg tgctgctcgt cccggtaggg tcccgggttg ccctggggga tgacgaggtc
    20821 ggtgtacggt gccagtgagc cgtcctcgtt gatcgggaac accgccacgc ccgggccgtt
    20881 ggacaggacg acgtagcggt tgcccggatc cggcatcacg gtggacgagt tgtcgccgtc
    20941 ggtttgctgc ctgttgagga accgaagctt gccggtctgc atgtcccgcg cgtaggcgcc
    21001 aatctccgcg ccgtcgccgt gggccgagta caggaaccgt tgcgtgcggt ccaggcagat
    21061 gtaggacggg ttcgggatgg cgtcgaacgc ctcgacgaac gtccatgcgc cggtagccgg
    21121 gtcgatccgg aacacggcga tgcctttgcc gcgggccctg cgcttttcgg tggtgaagca
    21181 accgacgtag gcaaacttcg gtcgccgacc gattcgaggt tcactcatca cccatcactc
    21241 atcacccatc actcgtcact cgtcactcga cactcgacac ccatcaccca actaatgcag
    21301 ggtcctgacc tcgggcggaa ctcgcagccc cacgttgagc accaccaccc gatgaccccg
    21361 catgaccacg ctgcccttga ggcggttgtt gccggtatcg ctgaattcga aatcgtaagt
    21421 gcgctggagc gtgaggctcc cgcgttcatc gcgcgccggc ctcaggccgg aaatcgccac
    21481 ggtgtagtcg agcaactgca gtccctccgc cgcgcagacc tcgcgggcgg cgcggatcgc
    21541 ggcttcccgc gccttcaggc tgtcgaacca gagccaggcg attcctccga gcacgaggat
    21601 gccaaagact tccaggccgg gcatgaaaaa cctgaggaag aaggacgaag gtggaagtat
    21661 gaaggaaaaa accgttaagc gttgccccct caccctctgc cctggttgga acggcgtgag
    21721 tcgtgattcg tgagtcgtg
//
LOCUS       DBNGWZ010000284         2033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2298818, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000284 DBNGWZ010000000
VERSION     DBNGWZ010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2298818"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="AC16CX_08385"
     CDS             <1..358
                     /locus_tag="AC16CX_08385"
                     /inference="COORDINATES: protein motif:HMM:NF016064.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HIR3012565.1"
                     /translation="FVAPMGDTLRYLLDPANLSLVNFGVAALAGVILGSTLWALFTGR
                     FRIEWFASWRDFANHAVGAALMGIGGVLSMGCTFGQAITGISTLAIGSILTFLAIVIG
                     AAGTMKYQYWRITREA"
     gene            485..560
                     /locus_tag="AC16CX_08390"
     tRNA            485..560
                     /locus_tag="AC16CX_08390"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:518..520,aa:Lys,seq:ttt)
     gene            802..1107
                     /locus_tag="AC16CX_08395"
     CDS             802..1107
                     /locus_tag="AC16CX_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012566.1"
                     /translation="MTFFVEGLSKHLEPQTQVRRIGEYKTLAEAVAAAQKVVDEFLRR
                     EFKPGMDAKALFSKYQSHGEYPFIFRDDDKTLNVPGFNHTHYAMTRCGQICGGKKPA"
     gene            complement(1167..>2033)
                     /locus_tag="AC16CX_08400"
     CDS             complement(1167..>2033)
                     /locus_tag="AC16CX_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012567.1"
                     /translation="VVGQAPGGATDVVARLVAPKFGEHLGQSVVVENRTGAAGSIGAA
                     FVAKSPPDGYNLLVISSSYAINPSLYKDLPFDPVKDLMPVTLIAEAPFLLVVHPSMPV
                     RSVKDLIAFAKAKPGVLNFASGGNGSSGQLAGELFKFLAGVSMVHVPYKGAGPAMVDV
                     IAGQVHMTFASVLSSLPHVKSNRLRALAVTGARRSAAVSELPTLAEAGVKGYRTTTWY
                     GLLAPANTPAPIVSRLSAEMQKVVESGDVKNRILSDGAEPAGGTPKQFQDHLASEMAR
                     AREIIQRAGVKP"
BASE COUNT          371 a          662 c          621 g          379 t
ORIGIN      
        1 tttcgtggcc ccgatgggcg acacgctccg ctatctgctc gatccggcga acctctcgct
       61 cgtcaacttc ggcgtggcgg cgctggccgg cgtgatcctc ggctctacgc tgtgggcgct
      121 cttcaccggc cggttccgca tcgagtggtt cgcctcgtgg cgcgatttcg ccaaccatgc
      181 cgtcggcgcc gccctgatgg gcatcggggg cgtgctctcc atgggctgca ccttcggcca
      241 ggcgatcacc ggcatctcca cgctcgcgat cggctccatc ctcaccttcc tcgccatcgt
      301 catcggcgcc gcggggacga tgaagtatca atactggagg attacgcgag aagcctgatc
      361 tcgcgtaatc ctgaattgga attcatcttc aaaaagccgc gcataatatc gctatccgcg
      421 gccggctcgc gagaatatct tcggtgaccc tacctgaccc cgtgggtaaa atgcgcggct
      481 ttttgggcgg atagctcagt cggtagagca gcggactttt aatccgttgg tccggggttc
      541 gaatccccgt ccgcctacca ataaaaaagc cggcgcacgc cggctttttt atttacatga
      601 aattgttgag gatactgaca ccaagcccga cgttcaccag caatggacgt taaccgaagc
      661 ccagtaaaat ctattcagtt taagaaaacc ggctcgtgcc ggttttcttc acgatttcct
      721 cacaaacttg cgccgtttac cgttgtatac tcgccgcaag gcgcggggaa gcgcgccacg
      781 aaagaatttc agagaggatc gatgactttc ttcgtcgaag ggttgagcaa gcacctcgag
      841 ccgcagaccc aggtgcgccg catcggcgaa tacaagacgc tcgccgaagc tgtcgccgcg
      901 gcgcagaagg tggttgacga gttcctgcgc cgcgagttca agccgggtat ggacgccaag
      961 gcgctgtttt ccaaatacca gagccacggc gagtatccgt tcatcttccg cgacgacgac
     1021 aagaccctca acgtgcccgg cttcaaccac acccactacg ccatgacccg ctgcgggcag
     1081 atctgcggcg gcaagaagcc ggcctgaacg gctctatccg gaagaccgcg cgcgaaggac
     1141 cggatcgcgc gcagtaccgg ccggtctcaa ggctttacgc cggcccgctg gatgatctcc
     1201 cgcgcgcgag ccatctcgga tgcgagatgg tcctggaact gcttcggcgt cccgcccgcc
     1261 ggctctgcac cgtcgctgag tatccggttc ttgacgtccc ctgactcgac caccttctgc
     1321 atctccgcgc tcagccggct cacgatcggg gcaggggtgt tcgcgggggc aagcagtccg
     1381 taccaggtcg tcgtccggta gcccttgacg cccgcctcgg cgagcgtcgg cagctccgaa
     1441 acggcggcag agcgcctggc gcccgtcacc gcgagcgccc gcagccgatt gctcttcacg
     1501 tggggcagtg aagagagcac gctggcgaag gtcatatgga cctggcccgc gatgacgtcg
     1561 accatggcgg ggccggcgcc cttgtacggc acgtggacca tgctcacccc ggccaggaac
     1621 ttgaacaatt cgcccgcgag ctggcccgag ctgccgttgc cgccggaagc aaagttcagc
     1681 acgccgggct tcgctttcgc aaacgctatg agatccttga ccgagcgcac cggcatcgag
     1741 ggatgcacca ccagcaggaa cggcgcttcg gcgatcagcg tcaccggcat gaggtccttg
     1801 accggatcga acggcaggtc cttgtacagg ctcgggttga tcgcgtagct gctcgagatg
     1861 acgaggaggt tgtagccgtc cggtgggctc ttggcgacaa aggccgctcc gatggagccg
     1921 gcggcgccgg tgcggttttc caccaccacc gactggccaa gatgctcgcc gaatttcgga
     1981 gcgacgaggc gggcgacgac gtcggtggcg ccgcccggtg cctgcccgac gac
//
LOCUS       DBNGWZ010000285         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_536363, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000285 DBNGWZ010000000
VERSION     DBNGWZ010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_536363"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="AC16CX_08405"
     CDS             <1..306
                     /locus_tag="AC16CX_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3012568.1"
                     /translation="VLACRPELVHLDRIDDSSDHTHGRKWDKLRRTRSYQPVLRDIRT
                     IAPTGWYGAPEHATVEKAKRMLDGIAGAIAKEAGEIFRLLDEAQGGTAEIKHLKKAS"
     gene            572..946
                     /locus_tag="AC16CX_08410"
     CDS             572..946
                     /locus_tag="AC16CX_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012569.1"
                     /translation="MAKVLTQAQAKSMGLPGRKSLEIVSGEKGSRAVTLRLVEIPVPQ
                     SGDKPRGPHYHKDFEECIFVMAGEGCTEADSGKHPLKAGDTILIPPGERHMTRNTGNM
                     PLVLLCFFPVADITKRTEEQKA"
     gene            1031..2008
                     /locus_tag="AC16CX_08415"
     CDS             1031..2008
                     /locus_tag="AC16CX_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012570.1"
                     /translation="MANDVLCLRPLADFQRVGVTPPASLAVNYRASDDPQLAAHVKAA
                     RALVIPAVGPRLAGALFEGGGVKLVQVTGAGVDRLDEAAMKKLGIAVANVPGGSNSAV
                     AEYVTGCAIVLLRRLTLADAEIRNANYVSIRGRLVADNLPGLEGLTVGIVGLGVIGLA
                     VAQAFKRYGCEILYYDPASRDADAAAMGAKAVPLDELLRSSDIVTVHVPLLPQTRSMI
                     GAKQLTMMKKGAVLIQASRGGVVNETALTAHLNSGHLGGAAVDVYSVEPPDTDNPLFA
                     LEGDAAYRVLFTPHLAGVTRQASATLFREAWANVERVLVKGEAPLNRVY"
     gene            2104..2712
                     /locus_tag="AC16CX_08420"
     CDS             2104..2712
                     /locus_tag="AC16CX_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3012571.1"
                     /translation="MSRVSPAPPERFAQVTGTMERWERTKGYPPNSWLTMVRKPGVFR
                     AYRDLHTAVMMEEGEIPRALKFMVAHVVSRACGDPYSAAHNAENAARIAGVPIEKVEA
                     LSQFQASPLFTPAERAALELALAAGGCPPAVTDAHFVGLRRHYGEDAIIEIVAVIALL
                     GWLNRWNNTLATRLEDEALVFARRHLAPSGWTPGVHIGEDAR"
BASE COUNT          510 a          938 c          918 g          432 t
ORIGIN      
        1 gtgctcgcct gccgccccga actcgtccac ctcgatcgca tcgatgactc ctcagaccat
       61 acgcacggcc gcaagtggga caagctgcgc cgcacccggt cctatcagcc ggtgctgcgc
      121 gacatccgca ccattgcccc gaccggctgg tacggcgcgc cggagcacgc gacggtggag
      181 aaggcgaaac gcatgctcga cggcatcgcc ggcgcgatcg cgaaggaggc cggtgagatc
      241 ttccgcctgc tcgacgaggc acagggtggc actgccgaga tcaagcatct caagaaggca
      301 agctgacgag catcgaaacc atgcgcaacg atgctctccc ctcatccctg cccgcggacg
      361 gcggagcagt gcctgcccgt atcacgggta ggtgcgaccc gggagtgggc aaaggcgcgc
      421 ggctcagaac cgcgggcatt acgcgcgctt gcacggtcgc atccccggaa accggggccc
      481 ctgctcctcg ctccagcgcg cccttctccc gagtggagaa agaagattcg aggcgcaaag
      541 gcgttgtcac gcttttgcgc ggagcctgac aatggcgaaa gtcctgaccc aggcccaggc
      601 gaagagcatg ggattgccgg gtcgaaaatc cctcgagatc gtctcgggcg agaaaggctc
      661 gcgcgcggtc acgctgcggc tggtcgagat ccctgtgccc caatccggcg acaagccgcg
      721 ggggccgcac tatcacaagg acttcgaaga atgcatcttc gtgatggcgg gcgagggctg
      781 caccgaggcc gacagcggga aacatccgct gaaagccggc gataccatcc tgatcccgcc
      841 cggggagagg cacatgaccc ggaataccgg caacatgccg ttggtcctgc tgtgcttttt
      901 cccggtggcc gatatcacga agcgtaccga agaacagaaa gcgtgagtcg tgaatcgaaa
      961 aatcctactc gccggctgac cgggtccctt ccctacaact cacgaatcac aaatcacggt
     1021 tcacgaagtt atggctaatg acgtcctgtg cctgcggccg ctggccgatt ttcagcgcgt
     1081 cggcgtgacg ccgcccgcgt ccctcgccgt caattaccgc gccagcgacg acccgcagct
     1141 cgcggcacac gtcaaggccg cgcgcgcgct ggtgattccg gcggtcggcc cccgcctggc
     1201 gggggcgctg ttcgagggcg gcggcgtgaa actggtgcag gtgaccggcg ccggcgtgga
     1261 tcggctcgat gaagcggcga tgaagaagct cgggattgcc gtcgccaacg tgccgggggg
     1321 cagcaacagc gccgtcgcgg aatacgtgac cggctgcgct atcgtcctgc tgcggcgcct
     1381 gacattggca gatgccgaaa tccgcaatgc gaattacgtt tcgatccgcg ggcgcctggt
     1441 ggccgacaac ttgcccggac tcgaagggct cacggtgggc atagtgggct tgggcgtgat
     1501 cgggctcgcc gtcgcgcaag cgttcaagcg ttacggatgt gagattctgt actacgatcc
     1561 cgcctcccgg gacgcggacg ccgccgctat gggcgcaaaa gcggtgccgc tcgacgagct
     1621 gctcaggtcg tccgacatcg tcacggtaca cgtgccgctg ctgccgcaga cccgcagcat
     1681 gatcggcgca aagcagctca ccatgatgaa gaagggcgcg gtgctgatcc aggcgtcgcg
     1741 cgggggcgtc gtaaacgaga ctgcgctcac cgcgcacctc aactcgggac acctgggcgg
     1801 cgcggcggtg gacgtttatt cagtcgagcc gcccgatacc gacaacccgc tgttcgccct
     1861 cgaaggcgac gccgcgtacc gggtgctgtt cacgccgcac ctcgcgggcg tgacccgcca
     1921 ggcttccgcc accctcttcc gcgaagcgtg ggccaacgtc gagcgcgtgc tcgtgaaggg
     1981 agaggcgccg ctcaaccgcg tctattgagc tgtcagcgtt cagccgtcag ccttcagctt
     2041 gcagcaaccc aatcacgacc ccgctgaccg ctgatagctg agaactgata accggagaat
     2101 tccatgtcac gtgtctcacc cgctccgccc gagcggttcg ctcaggtcac cggcaccatg
     2161 gaacgctggg agcgaaccaa gggctacccg cccaacagct ggctcacgat ggtgcgcaag
     2221 cccggcgtgt ttcgcgcgta ccgcgacctg catacggccg tgatgatgga ggaaggcgaa
     2281 atcccgaggg cgctgaagtt catggtcgcg cacgtcgtca gccgggcgtg cggggacccc
     2341 tattccgccg cgcacaacgc tgagaatgcc gcgcgcatcg cgggcgtccc gatcgagaaa
     2401 gtggaagccc tgtcgcagtt ccaggcaagc cccctgttca ccccggccga gcgcgctgcg
     2461 ctcgagctgg cgctcgccgc gggcggatgc ccgccagcgg tgaccgatgc gcacttcgtg
     2521 gggctcagga ggcactacgg cgaagacgcc atcatcgaga tcgtggcggt gatcgcactc
     2581 ctcggctggc tcaaccgctg gaacaacacg ctcgcgaccc ggctcgagga tgaggcgctc
     2641 gtcttcgcgc gccggcacct cgcgccctcg ggctggacgc ccggcgtgca catcggagag
     2701 gacgctcgat gaaactcaag gaactgggca tcgcagtcgt cggcgccggc cgcatcggca
     2761 cgctgcgggc gcggctcgct gcgaaacatc cgtgcgtc
//
LOCUS       DBNGWZ010000286         1783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1670626, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000286 DBNGWZ010000000
VERSION     DBNGWZ010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1670626"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16CX_08425"
     CDS             complement(<1..342)
                     /locus_tag="AC16CX_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012572.1"
                     /translation="MPTKVSLTVNGKPVTARVEGRTLLVQLLREHLGLTGTHVGCDTS
                     QCGCCTVHLDGKGVKSCTVLALACEGAAVTTIEGLAPEGGLHPMQEAFREHHGLQCGF
                     CTPGMIMSAVDM"
     gene            complement(402..1373)
                     /locus_tag="AC16CX_08430"
     CDS             complement(402..1373)
                     /locus_tag="AC16CX_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012573.1"
                     /translation="MPRFFLTCAWLSVLIAATLPAAHAQTGKWPDKTVRVIVPLAPGG
                     GNDIIARLFAARLTAEFGQQFIVDNRSGAGGSIGAEIAARANPDGYTLITVPASYAAN
                     AALYKLPYDPVKGIAPIAMIGTGPLILTVHPSVQATSLKEFIALARAKPGAIAFGSSG
                     NGTFSHLTAELFRQMSRTEMVHVPYKSAGPALIDLLGGQIQVFFGSGPSTSGHIRAGR
                     LRGLAVTTAKRSPALPDLPAIGELLPGYASDFWFGMWAPAGTPREIIMRLNQALGRIL
                     KQPEVLERLRTDGVEPVGSTPEEFARTIARDIATWSKVVKAGNIKVD"
     gene            complement(1382..>1783)
                     /locus_tag="AC16CX_08435"
     CDS             complement(1382..>1783)
                     /locus_tag="AC16CX_08435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012574.1"
                     /translation="IDTTIVATQLILAGVFDRFPKLRLVLVHGGGFLPYQTGRLDREF
                     GGKDKKLPSDYVKRFYYDTVFMSTPALQLLFSLVGTGQVMIGSDYAAGPVERPGPRLT
                     EALDATGIDAAARKTIVRDTAESLFRTAQGA"
BASE COUNT          291 a          580 c          601 g          311 t
ORIGIN      
        1 catgtccacc gccgacatga tcatgccggg cgtgcagaat ccgcactgca gcccgtggtg
       61 ctcgcgaaac gcttcctgca tcgggtgcag tccgccctcc ggcgcgagcc cttcgatggt
      121 cgtcacggct gcgccctcgc atgccagcgc gagcacggtg cacgacttca cgcccttgcc
      181 atcgagatgc acggtgcagc atccgcactg gctggtgtca caaccgacgt gcgttccggt
      241 gagccctagg tgctcgcgca gcagctgcac gaggagcgtc cgaccctcga ctcgcgcggt
      301 gacgggcttt ccgtttacgg ttagcgagac ttttgtcggc atctcgtctc cgttgctatt
      361 tcaagtaccg taacgcgtca ctcatcactc atcactcgtg actagtcgac cttgatgttc
      421 ccggccttga ccaccttgga ccacgtggcg atgtcgcggg cgatcgtgcg cgcaaactct
      481 tccggggtgg agcctacggg ctccacgccg tcggtgcgca gccgctccag cacctcgggc
      541 tgcttgagga tgcggccgag cgcctggttg agccgcatga tgatttccct gggcgtgccc
      601 gccggcgccc acatgccgaa ccagaagtcc gacgcgtagc cgggcaacag ttcgccgatc
      661 gcgggcaggt cgggcagcgc aggcgagcgc ttcgcggtgg tcacagcgag tccgcgcagc
      721 cggccggccc ggatgtgccc gctggtggat ggccccgagc cgaagaacac ttggatctgt
      781 ccgccgagca agtcgatcaa cgcgggaccg gcgctcttgt aaggtacgtg caccatctcg
      841 gtcctggaca tctgccgaaa gagctctgcc gtcaggtgcg agaaggtgcc gttgcccgac
      901 gagccgaagg cgatggcgcc gggcttggcg cgcgcgaggg cgatgaattc cttgaggctc
      961 gtcgcctgca ccgacgggtg cacggtgagt atcaacggcc cggtgccgat catcgcgatc
     1021 ggcgcgatgc ccttgaccgg atcgtagggc agcttgtaga gcgccgcgtt ggcggcatag
     1081 ctggccggca ccgtgatgag cgtgtagccg tccggattgg cgcgtgccgc gatctccgcg
     1141 ccgatcgagc cgccagcgcc ggaccggttg tcgacgatga actgctggcc gaactccgcc
     1201 gtcagccgcg ccgcgaacag gcgcgcgatg atatcgttgc cgccgccggg cgcgagcggg
     1261 acgattaccc gtaccgtctt gtcaggccat ttgcccgttt gggcgtgcgc agccggcaag
     1321 gttgcggcga tcagcactga cagccatgcg catgtcagga agaagcgggg catgaccggc
     1381 ctcacgcgcc ttgcgctgtc ctgaacaggg actcggcggt gtcgcgcacg atcgtcttgc
     1441 gggccgcggc atcgatgccg gtcgcgtcga gggcctccgt caatcgtggc cccgggcgct
     1501 cgaccggccc ggcggcgtag tcggaaccga tcatgacctg tcccgttccg accaggctga
     1561 acaggagctg gagcgcgggc gtcgacatga acaccgtgtc gtaatagaac cgcttgacat
     1621 aatcggaagg cagcttcttg tccttgccgc cgaattcacg atcgaggcga ccggtctggt
     1681 agggcagaaa accgccgccg tgcacgagaa caagccgcag tttgggaaag cggtcgaaca
     1741 cgccggcgag gatgagctgc gtggcgacga tcgtcgtgtc gat
//
LOCUS       DBNGWZ010000287         3348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1542896, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000287 DBNGWZ010000000
VERSION     DBNGWZ010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1542896"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1559
                     /locus_tag="AC16CX_08440"
     CDS             <1..1559
                     /locus_tag="AC16CX_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012575.1"
                     /translation="HELDKLGVKLLEVRHEGSGLGMADGWARATHEPGVATATCGPGV
                     SQLATALLTASRASSPIVAFCGEHPTNDDEYNQRLDQARFAAACETGFVRLNTPDGAD
                     DAVRKAFYLAKLESRPVMLSAPMDIQQKNFDDDEPYQPSSTLFPSRVVHPDPDALGRA
                     ADIIAGSKKPVIVVGRGAKWSGAGDAVLKLAERVGAVIATTLMAKTWLSHAEYHVGVS
                     GTYATRTAMQLFEEADCVIAVGASLNRYTTEHGYLYPNARFVHLDSKPHVMMYGSRSA
                     DCYIQTDARAGVEALERLLAGRSVKMTGYRTPDVKKRLVHNYEDRAEFPVEAGTVDPR
                     EVIRALDEIVPANVDLYTGSGASAGFANILFKHHRPVVLASHFFGCIGQMLPAAMGAV
                     VALGNRPGLLVDGDASIMMHLAEFETMVRYNMPLLAVCMNNQALGSEYYKLDVKKMNA
                     GLATIPTPDLGAVAKAFGGKGRLARSVEDVSAAAAEWVAKPAPMMIDARISRSVLTLP
                     YRRIHYGRDE"
     gene            1595..2677
                     /locus_tag="AC16CX_08445"
     CDS             1595..2677
                     /locus_tag="AC16CX_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012576.1"
                     /translation="MRPAGMFALLCALGAASSAPAALAQSGKYPDRPIRLISPFPPGG
                     TVDVNARILAPAVSKSIGQQVVVDNRSGASGIIGTAAAAREKPDGYTLLLNTTPMVTN
                     TLMYSKVPYNVLKDFEPISIVSTTPSFLGVHPSVPARSVRELLQLAKSRPGELNYASA
                     GIGTNPHISGELFNYLGKTNIVAVQFKGGGPALVAAISGEVGVAFSGVAGAVGFVHSG
                     RLRALGVTSLKPVAALPGVPPIAQAGLPGYEFVAWFVLAAPKGTPREIILTLNDHFRK
                     AMSFPDTIRRFEAEGLDVVGSTPEQAAATLESELKKWAVVIKERGMRADQALEEIPSF
                     LALFTYQGTRRTRWRQPGSPIPAPCG"
     gene            complement(2607..2846)
                     /locus_tag="AC16CX_08450"
     CDS             complement(2607..2846)
                     /locus_tag="AC16CX_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012577.1"
                     /translation="MFAIICKADGFPVSRQVGDDKPSLVVTWTSDAAAKAFLSAKGIE
                     ADYQVVALTDESLNRMAQVLGCPADAIAFDAYPDK"
     gene            complement(2941..>3348)
                     /locus_tag="AC16CX_08455"
     CDS             complement(2941..>3348)
                     /locus_tag="AC16CX_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3012578.1"
                     /translation="VSKNSYKDFEIYAEFWAETSTNSGIHIRNADPKKISSATSYEVN
                     IYDQRPDPSYGTGAIVGVAMVNPMPKAGGKWNIYEIYAKGPQLIIKLNGVVTASIQDS
                     KFASGPFTLQYGSGAKGAPGGAIKWRKVMIRSL"
BASE COUNT          585 a         1078 c         1095 g          590 t
ORIGIN      
        1 tgcacgagct ggataagctc ggcgtcaagc tgctcgaagt ccgtcacgag gggtcaggcc
       61 tcggcatggc ggacggttgg gcgcgggcga cgcatgaacc cggggtcgcc accgcgacct
      121 gcggcccggg cgtgtcgcag ctggctaccg cgctgctgac tgcaagccgc gccagctctc
      181 cgatcgtcgc cttttgcggc gagcacccga cgaacgacga cgagtacaac cagcggctcg
      241 accaggcgcg gttcgccgcc gcctgcgaga ccggcttcgt ccggctcaat acgcccgacg
      301 gcgcggacga cgccgtccgc aaggcgttct acctggcgaa gctcgagtcc cgccccgtca
      361 tgttgagcgc gccgatggac atccagcaga agaacttcga cgacgacgag ccgtaccagc
      421 catcctccac gctgttcccg tcgcgcgtcg tgcacccgga tcccgacgcg ctcgggcgtg
      481 cggcagacat catcgccggg agcaagaagc cggtgatcgt ggtcgggcgc ggagcgaagt
      541 ggtcgggcgc aggcgatgcg gtgctcaagc tcgcggagcg cgtcggggcg gtgatcgcca
      601 ccacgttgat ggcgaagacc tggctgagcc acgccgaata ccacgtcggt gtttccggca
      661 cctacgccac gcgcacggcc atgcagctgt tcgaggaggc cgattgcgtc atcgccgtcg
      721 gcgcgagcct caaccgctac acgaccgagc acggttacct gtatcccaac gcgcgcttcg
      781 tgcacctgga ctccaagccc cacgtcatga tgtacggcag ccgctccgcc gattgctaca
      841 tccagaccga tgcgcgcgcc ggcgtcgaag ccctggagcg cctgctggcg ggccggtcgg
      901 tcaagatgac gggctaccgg accccggacg tcaagaagcg gctcgtccac aactacgagg
      961 accgggcgga gtttccggtc gaagcgggaa ccgtggatcc gcgcgaggtc atccgcgccc
     1021 tcgacgagat cgtgcctgcc aacgtcgacc tctatacagg gagcggggcg agcgcgggct
     1081 tcgcgaacat cctcttcaag caccaccgcc cggtggtgct cgcgagccac tttttcggct
     1141 gcatcgggca gatgctgccc gccgcgatgg gggcggtggt ggccctgggc aaccggccgg
     1201 gcctgctcgt cgatggcgac gccagcatca tgatgcacct cgccgagttc gagacgatgg
     1261 ttcgctacaa catgccgctg ctggcggtct gcatgaacaa ccaggcgctc ggctcggagt
     1321 attacaagct cgatgtgaaa aagatgaacg ccggtctcgc gaccattccg acaccggatc
     1381 tgggcgccgt tgcgaaggcc ttcggcggca aaggccggtt ggcgcgaagc gtcgaagacg
     1441 tgagcgccgc cgccgccgaa tgggtggcca agcccgcccc gatgatgatc gatgcgcgga
     1501 tttcccgaag cgtgctaacc ctgccttacc gccgcattca ctacggccgg gacgaatgac
     1561 ccgtcaacgt caaccagcca agggggaatg catcatgaga ccggcgggca tgtttgcgct
     1621 gctttgcgca ttgggcgcgg catcatcggc gccggcggca ctggcgcaat cggggaagta
     1681 tcccgaccgc ccgattcgct tgatctctcc gtttccgccg ggcggcaccg tggacgtgaa
     1741 tgcgcggatc ctggcgccgg ctgtatcgaa gtcaatcggc cagcaggttg tggtggacaa
     1801 tcgctccgga gcgtccggca tcatcggtac ggcggcggcg gcgcgcgaaa agccggacgg
     1861 ctacacgctt ctcctcaaca ccaccccgat ggtgacgaac acgctgatgt acagcaaggt
     1921 gccatacaac gtgctcaagg actttgagcc gatctcgatc gtgtcgacga caccgtcgtt
     1981 cctgggcgtg catccgtcgg tgccggcccg gtctgtgcgc gagctgctgc agctcgcgaa
     2041 atcccgcccc ggcgagctca actacgcctc cgcgggcatc ggcacgaatc cgcacatttc
     2101 cggcgagctg ttcaactacc tcggcaagac gaacatcgtc gccgtccagt tcaagggcgg
     2161 cggccccgcg ctggttgcgg cgatcagcgg cgaggtgggc gtcgcgtttt caggtgttgc
     2221 cggcgcggtg ggtttcgtgc attcgggccg cctgcgggcg ctcggcgtga ccagcttgaa
     2281 acccgtggcg gcgctgcccg gggttccgcc gatcgcccag gccgggctcc ccggctacga
     2341 gttcgtcgcg tggttcgtgc tggccgcgcc caagggtacc ccccgcgaaa tcatcctcac
     2401 gctgaacgac cacttccgga aggcaatgag ctttcccgat acgatccggc gattcgaggc
     2461 ggaagggctc gatgtcgtcg gcagcacgcc ggagcaggcg gcggcgacct tggaatccga
     2521 gctgaagaag tgggcggttg tgatcaagga aaggggcatg cgcgccgatc aggctctcga
     2581 ggaaataccc tcattcctcg cccttttcac ttatcagggt acgcgtcgaa cgcgatggcg
     2641 tcagccgggc agcccaatac ctgcgccatg cggttgagag attcatcggt gagcgcgacc
     2701 acctggtagt cggcctcgat gcccttggcg gagagaaagg cctttgccgc ggcgtcggaa
     2761 gtccaggtca caacgagact cggtttatcg tctccaacct ggcggctcac cggaaagccg
     2821 tcggctttac agatgattgc aaacatgcgt tagtctccgc tggcttcgtt cggtgacgga
     2881 gtgacgaggt gacgggttta acccccatca cccatcactc atcacccatc acgctcatgc
     2941 ctaaagcgac ctgatcatca ccttgcgcca cttgatggcg ccgccggggg cgcctttggc
     3001 accgctaccg tactggagcg tgaacggccc ggaagcaaac ttgctgtcct ggatgctagc
     3061 ggtgacgacc ccgttgagct tgatgatcaa ttgcgggccc ttggcgtaga tctcgtagat
     3121 gttccatttg ccgccggcct tgggcatggg attcaccatg gcgacgccga cgatggctcc
     3181 cgtgccgtag ctcgggtcgg ggcgctggtc atagatattc acttcatagg aagtggcgga
     3241 ggagatcttc ttgggatcgg cattgcggat gtggatgccg ctgttggtag aggtttcggc
     3301 ccagaactcg gcatagatct cgaaatcctt gtacgagttt ttcgagac
//
LOCUS       DBNGWZ010000288         4068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1670765, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000288 DBNGWZ010000000
VERSION     DBNGWZ010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1670765"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16CX_08460"
     CDS             <1..672
                     /locus_tag="AC16CX_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HIR3012579.1"
                     /translation="QLEIWQGRLNLASRASSFKSISAVVVFEGNDAAGKGGAIRRVTS
                     ALDARCYTNVSVAAPTEEERAQPYLWRFWRHIPRHGRVTFFDRSWYGRVLVERVEGFC
                     SEADWMRAFTEINDFEGALLRHGSVVVKFWLAISKDEQFKRFKAREKIAFKRFKITEE
                     DWRNRKKWDAYEQAVCDMVDRTSTSIAPWTLVEANNKYYARIKVLRTLTRAVEAALKR
                     AKKQG"
     gene            676..2694
                     /locus_tag="AC16CX_08465"
     CDS             676..2694
                     /locus_tag="AC16CX_08465"
                     /inference="COORDINATES: protein motif:HMM:NF011314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR3012580.1"
                     /translation="MRRTGDVARETPAPAAVAVTAGGPIVPRNRLPLARSRRQPAPAQ
                     AADRVWLGSIKDIRGLLERMQTLRAAVIAAGSAFAKSWRPYLRKREYYPSAANLAAYI
                     GLRRHDLRSLQGYLATLGLSSLGRCEGHVMATLDAVINALKQMSGKRVLRRRVANVAR
                     AMARDEVLLRHHTNRLFGPPPANRWTRFMVTLPTEAATDEGFVRELVKHGMDCARINC
                     AHDDPAAWRAMVRNVRRAERETGRTCRILMDLGGPKLRTGPVETGPPLLRIRVKCDAR
                     GVPAQPARLLLDASGRPGRPAANGKAGSPTARLTVERAWLNRLQKGDTIRFIDLRKRE
                     RRLAVEKRLSNAACVASTARGAWIGADTVLEHVPRRGRRPAGATSTVGGIAAQPLEIR
                     LEQDDLLLLTRAQTPGEPKRGDGRGKTVAPAHISCSEPRIFSALKVGHHVWIDDGKIQ
                     TVVEVLDEHGAWLRVTHARPGGDRVGPGKGLNFPDSELPLPALEESDLADLDFAARRA
                     DIIGFSFVRTAGDMDALIQALASRGRHNLGIIAKIETRAAVKNLPEIIIRGAGSHPFG
                     VMVARGDLAVEIGYERLAEIQEEILWLCEAAHVPVIWATQVLEGMVKRGRPSRAEISD
                     AAMAGRAECVMLNKGPYVCEALSVLDSVVTRMRHHQRKKTAQFRALHW"
     gene            complement(2737..>4068)
                     /locus_tag="AC16CX_08470"
     CDS             complement(2737..>4068)
                     /locus_tag="AC16CX_08470"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3012581.1"
                     /translation="FGERLRDFDKHAVRAVGTNTLRVAKNAPAFLKKAEAALGFPIEV
                     VAGREEARLIYIGVSHELPATREKRLVVDIGGGSTEIIIGSGYKPLKLESLFMGCVSY
                     SLRFFEGGKVTKGAMKEAELAARNELQPIVAKFVRGNWQQTVGSSGTVRAIGDVIQLN
                     GFGESGITPAGLDRLRSHLIKAGDMDKVELAGLKPERVPVFAGGLAILNAVFSELDID
                     SMAVANGAMRQGVLYDLLGRFHHKDMRDVTVAQFMQRYHVDALQARRVGALAFALYRM
                     LTAEAGEPDEDVSRYITWASKLHEIGISVAFSGYHKHSAYILGNADMPGFSRDEQARL
                     ALLVQAHRRSLDKVEKQLDEHEVDWNMVFALRLAALFYRSRADIALPPLQTRRQGKKF
                     RLTVEAGWLARNPLTVTALHDEIREWDRAGFELKIPELEGLETSPDLALAS"
BASE COUNT          674 a         1387 c         1384 g          623 t
ORIGIN      
        1 cagctcgaga tatggcaggg gcgcctcaat ctcgcgtcgc gcgcctcgag tttcaagtcg
       61 atctcggctg ttgtggtatt cgagggcaac gacgccgcgg gcaagggcgg cgcgatccgg
      121 cgcgtcacca gcgcgctcga tgcgcgctgc tacaccaacg tttcggtggc ggcgccgacc
      181 gaggaagagc gcgcgcagcc ctacctgtgg cggttctggc ggcacattcc gcgccacggc
      241 cgcgtcacgt tcttcgaccg ttcctggtac gggcgggtgc tcgtcgagcg cgtcgaggga
      301 ttctgctccg aggccgactg gatgcgcgcc tttaccgaga tcaacgactt cgaaggcgcg
      361 ctcctgcgcc acggctccgt ggtggtgaag ttctggctcg cgatcagcaa ggacgagcag
      421 ttcaagcgct tcaaggcgcg cgaaaagatc gcgttcaagc gtttcaagat caccgaagag
      481 gactggcgca accgcaagaa atgggacgcc tacgagcagg ccgtgtgcga catggttgac
      541 cgcacctcga cctcgatcgc gccgtggacg ctggtggagg ccaacaacaa gtattacgcg
      601 cggatcaagg tgctgaggac cctcacccgc gccgtcgagg cggcgctcaa acgcgccaag
      661 aaacagggtt gatccatgag acgcacgggg gacgtcgccc gcgaaacccc cgccccggcc
      721 gccgttgctg tgaccgccgg cgggccgatc gtgccgcgga acagactccc cttggcgcgc
      781 tcgcggcgac agcccgctcc ggcccaggct gcggatcgag tctggctggg cagcatcaag
      841 gacatacgcg gtctgcttga gcgcatgcaa acgttgcgcg ccgcggtgat tgcggcaggc
      901 agcgcgttcg ccaagagctg gcggccgtac ctgcgcaagc gcgagtacta cccgagcgcg
      961 gccaacctcg ccgcctatat cggcctgcgc cgccacgacc tgcgatcgct ccagggctac
     1021 ctggcgaccc ttggattgtc ctctctcggg cgctgcgaag gccacgtcat ggccacgctc
     1081 gacgccgtga tcaacgcgct caagcagatg agcggcaagc gcgtcctgcg gcggcgcgtc
     1141 gccaacgtgg cgcgcgcgat ggcgcgcgac gaagtgctgt tgcgccacca taccaaccgc
     1201 ctgttcggcc cgccgccggc aaatcgctgg acgcgcttca tggtgacgct gccgaccgaa
     1261 gccgcgacgg acgagggttt cgtgcgcgag ctggtgaaac acggcatgga ctgcgcgcgc
     1321 atcaattgcg cccacgatga ccccgctgcc tggcgcgcga tggtccgcaa cgtcaggcgc
     1381 gcggaacgcg agaccggccg gacgtgccgc atcctcatgg atctgggagg gccgaagctc
     1441 cgcaccgggc cggtcgagac cggcccgccg ctgttgcgca tcagggtcaa gtgcgacgcg
     1501 cggggcgtgc cggcccagcc ggcgcggctg ctgctcgacg catcggggcg tcccggccgg
     1561 cccgccgcga acggtaaagc cggcagcccg accgcccgac tcaccgtcga gcgcgcctgg
     1621 ctcaaccggt tgcagaaggg cgacaccata cgcttcattg acctcaggaa acgcgaacgg
     1681 cggctcgcgg tggaaaagcg gctgtcgaat gcggcgtgcg tggcgagcac ggcccgtggc
     1741 gcctggatcg gggccgacac ggtgctcgag cacgtgccac gtcgcggcag gcggcccgcc
     1801 ggcgcgacca gcacggtcgg cggcatcgcc gcgcagccgc tcgagatccg cctcgagcag
     1861 gacgatctcc tgctgctcac ccgtgcgcag acgccgggcg agccgaagcg cggagacggg
     1921 cgcggcaaga ccgtcgcccc agcgcacatc tcgtgcagcg agccgcggat attctcagcg
     1981 ctcaaggtcg gccatcacgt ctggatcgac gacggcaaga tccagacggt ggtcgaggtg
     2041 ctcgatgagc acggtgcatg gttgcgcgtc acgcatgccc gccccggcgg cgaccgcgtc
     2101 ggccccggca aggggctcaa tttccccgac agcgagctgc cgctgcccgc gctcgaggaa
     2161 tccgacctcg cggatctcga ctttgccgcg cgccgcgcgg acatcatcgg cttctcgttc
     2221 gtgcgcaccg ccggcgacat ggacgcgctg atccaggcgc tcgcgagccg aggacgccac
     2281 aacctcggaa tcatcgccaa gatcgagacg cgcgccgcgg tgaagaatct gcccgagatc
     2341 atcatccgcg gcgcgggcag ccatccgttc ggcgtaatgg tcgcccgcgg ggacctcgcc
     2401 gtcgagatcg gctacgagcg actggcggaa atccaggagg agatcctgtg gttgtgcgaa
     2461 gcggcgcacg tgccggtgat ctgggcgacc caggtgctgg aagggatggt caagcgcggc
     2521 cggccgtcgc gcgccgagat ctcggacgcc gcgatggccg ggcgcgccga atgcgtgatg
     2581 ctcaacaagg gcccgtacgt gtgcgaggcg ctgtcggtgc tcgacagcgt ggtgacgcgg
     2641 atgcggcatc accagcgcaa gaagaccgcg cagttccgcg cgctgcactg gtagcggaaa
     2701 cggctgcccc gggcgcgggg cggcttgaag gccgtttcag ctcgcgaggg caagatcggg
     2761 gctcgtttcc agcccctcaa gctcggggat cttcagctcg aagccggccc tgtcccactc
     2821 cctgatctcg tcgtgcagcg cggtcacggt gagggggttg cgggcgagcc agccggcttc
     2881 aacggtcagg cggaatttct tcccctggcg tctggtttgc aggggcggca gcgcgatatc
     2941 cgcccggctg cggtagaaca gcgcggccag ccgcaacgcg aacaccatat tccagtccac
     3001 ctcgtgctcg tcgagctgtt tttccacctt gtcgagggac cttcgatgcg cctgcaccag
     3061 cagcgcgagc ctggcttgct cgtcgcgcga gaaaccgggc atgtccgcgt tgccgagaat
     3121 ataggcggag tgcttgtgat aaccgctgaa cgcgaccgaa atgccgatct cgtggagctt
     3181 cgacgcccag gtgatgtagc gtgaaacgtc ttcgtcgggc tcgcccgcct ccgcggtcag
     3241 catgcggtag agcgcgaagg cgagcgcgcc cacgcgccgc gcctgcaacg catccacgtg
     3301 gtatcgctgc atgaactggg cgaccgtgac atcgcgcatg tctttgtgat ggaagcggcc
     3361 gagcagatcg tagagcacgc cctggcgcat ggcgccgttc gccaccgcca tggaatcgat
     3421 gtcgagttcc gagaacaccg cattgaggat cgcgagcccc ccggcgaaca ccggcacccg
     3481 ctcgggcttc aagcccgcga gctccacctt gtccatgtcg cccgccttga tcaggtggga
     3541 gcgcaggcgg tcgaggcccg ccggcgtgat gccgctctcg ccaaagccgt tcagctggat
     3601 gacgtccccg atcgcgcgca ccgtgccgga ggaacccacc gtctgctgcc agttgccgcg
     3661 cacgaatttc gcgacgatcg gctgcagctc gttgcgcgcg gcgagctcgg cctctttcat
     3721 tgcgcccttg gtgaccttgc cgccctcgaa aaaacgcagg ctgtaggaca cgcagcccat
     3781 gaacaggctt tcgagcttga gcggcttgta gcccgagccg atgatgatct cggtggagcc
     3841 cccgccgatg tccaccacca gccgcttctc gcgcgtggcc ggcagctcgt gcgagacccc
     3901 gatatagatc aagcgggcct cttcgcggcc cgccaccact tcgatcggga agccgagcgc
     3961 agcctcggcc ttcttgagga acgccggggc gttcttcgcc acccgcagcg tattggtgcc
     4021 gacggcgcgc acggcgtgct tgtcgaaatc gcgcagccgc tcgccgaa
//
LOCUS       DBNGWZ010000289         3425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_408570, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000289 DBNGWZ010000000
VERSION     DBNGWZ010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_408570"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..679
                     /locus_tag="AC16CX_08475"
     CDS             254..679
                     /locus_tag="AC16CX_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3012582.1"
                     /translation="MANITRFSPIDDAFDDLFRGFFVRPLGFESPAGAAQFRVDVSEN
                     DGAYMLRAELPGVKKDDINVTIDGDTVAISAEVKNEKDVKDGERVLRSERYHGKVYRA
                     FTLGQAVDEAGANAKYANGVLELTLPKKAAVQVKRITIQ"
     gene            851..1738
                     /gene="argB"
                     /locus_tag="AC16CX_08480"
     CDS             851..1738
                     /gene="argB"
                     /locus_tag="AC16CX_08480"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR3012583.1"
                     /translation="MALTGNTAAEKAKILAEALPYIRRFHGKTIVIKYGGNAMTDDKL
                     KNSFARDVVLLKLVGINPVVVHGGGPQIDELLKRVGKKGEFVQGMRVTDAETMDLVEM
                     VLGGQVNKEIVNLINQHGGRAVGLTGKDGGLIRARKMLVRGAGDSDELLDIGQVGEVE
                     SIDPEIVHRLHTHDFIPVIAPLGVGKKGETYNINADLVAGKVAEVLKAEKLIVLTNTP
                     GVLDKNGNLLTGLSARKIDELFANGTISGGMLPKISSALDAVKNGVNTCHIIDGRVEH
                     ALLLEVLTDEGVGTLIRRR"
     gene            1832..2473
                     /locus_tag="AC16CX_08485"
     CDS             1832..2473
                     /locus_tag="AC16CX_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR3012584.1"
                     /translation="MWGRAWIFDLDNTLHNARPHIFPHINRAMTAYLQAHLGLDEAAA
                     GELRRRYWKRYGATLLGLMRHHETDPRHFLWHTHQLPELDRMLVHEPQLRATLRRLPG
                     SKLVFSNSPVHYSRAVLKSLAIADLFDDVFSIERTRYRPKPDSDGFLRLCRANHLKPQ
                     RCIMVEDSLENLRTAGRLGMKTVWVTRAARAPGYVDVKVADLAQLPRMLAYLQ"
     gene            2499..3086
                     /gene="slmA"
                     /locus_tag="AC16CX_08490"
     CDS             2499..3086
                     /gene="slmA"
                     /locus_tag="AC16CX_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HIR3012585.1"
                     /translation="MAAKTGERKDQILQTLAQMLENPAGEKVTTAALAARLSVSEAAL
                     YRHFRGKAEMFEGLIEFIEQTLFALINKITSEEKSGVRQIEAIVTVLLGFAQKNRGMT
                     RVLIGDALVNEDDRLQLRINQLHDRLEAAIKQALRFGVTRQELAADVDVAAHANLLMS
                     FVTGRWHQFAKSGFKRDPAELWAKQWRQLMEGVIA"
BASE COUNT          710 a         1071 c         1126 g          518 t
ORIGIN      
        1 ctcaagccgg gcgagaaagt gccgctcacg ctcaccatcc agcgcgccga tttcagccgt
       61 gccgtgttca cggtgcaggc cgaagtgcgc gaggccgccg ttgcgaaggc gcatcaacac
      121 tgatcacggc ataggaaaaa acaagaaaga tcaatccatt gcgccacggc cggcggccgg
      181 ccttgaaaac cccttcccgg ctcccatatt cgaggtaacg gcgctcagcg cccaactgaa
      241 caaggagacg gatatggcaa acatcacacg cttcagcccg atcgatgatg cgtttgacga
      301 tctgttccgc ggtttcttcg tgcgtccgct cggcttcgaa agccccgccg gcgccgcgca
      361 gttccgcgtg gacgtgtcgg aaaacgacgg cgcttacatg ctgcgcgccg agttgcccgg
      421 cgtgaagaag gacgacatca acgttacgat cgacggcgac acggtcgcga tcagcgcgga
      481 agtgaagaac gagaaggacg tgaaggatgg cgagcgcgtg ctccgttccg agcgctatca
      541 cggcaaggtg tatcgcgcgt tcacgctcgg acaggcggtg gacgaagccg gtgccaacgc
      601 caagtatgcg aacggcgtgc tggaactgac gctgccgaag aaggccgcgg tgcaggtgaa
      661 gcgcatcacc atacaataaa agttaaaccc ggacagcggc cgctgccggt ttaactccgc
      721 gcgttcgaac agacgaaacg ggttccgccg ttcaacgcgc ggaaagcagg gaagcgaaac
      781 ggcgggtcga gagacctgcc gttttttttc ccgtagaata gccgccgtcc cgcaacagca
      841 accgatactg atggccctga ccggcaacac cgccgccgaa aaggcgaaga tcctcgccga
      901 agcgctgccg tacatccggc gctttcacgg caagaccatc gtcatcaagt acggcggcaa
      961 cgcgatgacc gacgacaagc tgaagaacag cttcgcgcgc gacgtcgtgc tgctcaagct
     1021 cgtcggcatc aacccggtgg tggtgcacgg cggcggcccc cagatcgacg agctcctgaa
     1081 gcgcgtcggc aagaaaggtg aattcgtcca gggcatgcgg gtgacggacg ccgaaaccat
     1141 ggacctcgtc gagatggtgc tcggcggcca ggtcaacaag gagatcgtca atctcatcaa
     1201 tcagcacggc ggccgcgccg tcgggctcac cggcaaggac ggcgggctga tccgggcgag
     1261 aaagatgctg gtgcgcggcg ccggcgattc cgacgagctg ctcgacatcg ggcaggtggg
     1321 ggaggtcgag agcatcgatc cggagatcgt gcaccggctg cacacgcacg atttcatccc
     1381 ggtgatcgcg ccgctcggcg tgggcaagaa aggcgagacc tacaacatca acgccgacct
     1441 cgtcgcgggc aaggtagccg aggtgctgaa ggccgagaag ctgatcgtgc tcaccaacac
     1501 gccgggcgtg ctcgacaaga acgggaatct cctgaccggc ctctccgcca ggaagatcga
     1561 cgagctgttc gccaacggca ccatctccgg cggcatgctg cccaagatca gctcggcgct
     1621 cgacgcggtg aaaaacggcg tcaacacctg ccatatcatc gacgggcgcg tcgagcacgc
     1681 gctgctgctc gaggtgctca ccgacgaggg cgtcggcacg ctgatccggc gccgctagga
     1741 gctcgccggg ccgaaatccg gcgaattcct gaatcggggt aactttgata agcagcttgt
     1801 cgctgcggca ctgatccagg acgcatcaaa aatgtggggg cgcgcgtgga tttttgacct
     1861 ggacaacacc ctgcacaacg cgcgccccca catttttccg cacatcaacc gcgcgatgac
     1921 ggcgtacctc caggcgcatc tgggcctcga cgaggccgcg gccggcgagc tgcggcggcg
     1981 gtactggaag cgctacgggg cgaccctgct cggcctcatg cgtcaccacg agaccgatcc
     2041 ccggcacttc ctgtggcaca cgcaccagct gccggagctc gacaggatgc tcgtgcacga
     2101 gccgcagctg cgggcgacct tgaggcgcct gcccggcagt aagctcgtgt tctccaactc
     2161 gccggtccac tactcgcgcg cggtgctgaa atcgctcgcg atcgccgacc tcttcgacga
     2221 cgtgttctcg atcgagcgca cgcgctatcg ccccaagccc gacagcgacg ggttcctgcg
     2281 cctgtgccgc gccaaccacc tcaagccgca acgctgtatc atggtggagg attccctcga
     2341 gaacctgcgc accgccggcc ggctcggcat gaagacggtg tgggtgacgc gggcggccag
     2401 ggcgccggga tacgtggacg tgaaggtcgc ggatctggcg cagttgcccc gtatgctggc
     2461 gtatttacaa taggcgaccg gtacccggcg agaccgagat ggcggcgaag acgggcgagc
     2521 gcaaggacca gatactgcaa acgctggcgc agatgctgga gaatccggcg ggcgagaaag
     2581 tgaccacggc cgcgctggcg gcgcgcttga gcgtgtcgga agccgcgctc taccgccatt
     2641 tcaggggcaa ggccgagatg ttcgaggggc tgatcgaatt catcgagcag acattgtttg
     2701 cgctcatcaa caagatcacc agcgaggaga agagcggcgt gcgccagatc gaggcgatcg
     2761 tcacggtgct gctcggattc gcgcagaaga accgcggcat gacgcgcgtg ctcatcggcg
     2821 acgcgctggt caacgaggac gaccggctgc agctgcgcat caaccagctg cacgaccggc
     2881 tggaggccgc gatcaagcag gcgctgcgct tcggcgtcac gcgacaggag cttgccgccg
     2941 acgtggacgt cgccgcccat gcgaacctgc tcatgagttt cgtcaccggg cgctggcacc
     3001 agttcgcgaa gagcggcttc aagcgcgacc cggccgagct gtgggcgaag cagtggcgtc
     3061 agctcatgga aggcgtgatt gcataggcgc gctgtaacgt ggagcagggg ccccagtggt
     3121 ggggatgcga ccgtggaggc gcgcaggcgg ccgacacgat gtcatcatgt gcgcgacgac
     3181 cattcagagg ccgccagcgg tccgctttca gctatcagcc ccgaccccgg gaaacgcttt
     3241 agctgactgc tgaatgctga gtgctgatgg ctatatatag ggaggtgaat gatggcgaac
     3301 ctgacgaacc aacaactcgc ggaactgctg atcggcatcg cgcgcgcgca gcaggcgatg
     3361 atagacgcca tcgaaagcca gagggcgggc tacaagcaga cgcacctggg gccggccctc
     3421 acgac
//
LOCUS       DBNGWZ010000290         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2676157, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000290 DBNGWZ010000000
VERSION     DBNGWZ010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2676157"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="AC16CX_08495"
     CDS             complement(<1..595)
                     /locus_tag="AC16CX_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3012586.1"
                     /translation="MFLQQLINGLTLGGVYALVALGYTMVYGILELINFAHGEIYMLG
                     AYIGIIALGLFTALGWTEAAPGLSLIGAVAAAAVYCGAVGATMERLAYRPLRRAHRLA
                     PLISAIGVSIVLQNVVMLTQGSADKVFPRLSDAPTTFAVAGATVSALQLWIVGVSAAL
                     MAGLNVLIRKTRLGLAMRATTQDPRMASLVGVPVDRVI"
     gene            complement(620..1765)
                     /locus_tag="AC16CX_08500"
     CDS             complement(620..1765)
                     /locus_tag="AC16CX_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3012587.1"
                     /translation="MSRSLLTSRFRGRVIGGVSAALSIAFIVACAPKVSNEIKIGAAG
                     PMTGDQSKMGIDLRHGVELAVDEWNARGGVLGKRIVVQVEDDQHDPKQAVAVANKFVN
                     SGVVGVIGHWNSSASIPASGVYNQAKIVMITPASTNPRLTEQGFPTIFRVCGRDDQQG
                     PLAARFVRERFPGAAVAVLHDKTTYGQGLAEEFQRALGGGPRVVFGSGITQGDKDFRA
                     VLTTVKGAKPDVIFFGGIYPEAALLVRQARELGLAAPFISGDGTYDEKFLEIAGPAAD
                     GTYLTFGPDPADLPQSQAFLTKYHERFGPHGPYSIYAYDAANILLSAIAQAGTTDGLK
                     LAEVIRGTPHQGAQGEIRFDAKGDVLQAPYIVWIVKHGRFEQFWKPS"
BASE COUNT          315 a          614 c          657 g          293 t
ORIGIN      
        1 tgatcacccg gtccaccggc acgccgacca ggctcgccat gcgggggtcc tgcgtggtcg
       61 cgcgcatcgc gagccccaac cgggttttgc ggatcagcac gttcaacccc gccatcagcg
      121 cggcggacac gcccacgatc cacagttgca gcgcgcttac ggtggcgccg gcgacggcga
      181 acgtggtcgg ggcgtcgctc aggcggggaa agaccttgtc ggccgagccc tgggtcagca
      241 tcaccacgtt ctgcagcacg atcgacacgc cgatcgcgga gatcaagggc gccaggcgat
      301 gggcgcggcg cagggggcgg tacgccagcc gctccatcgt cgcgcccacg gcgccgcagt
      361 agacggccgc ggccgccacg gcgccgatca gcgaaagccc cggcgcggcc tcggtccagc
      421 ccagcgcggt gaagaggccc agcgcgatga tcccgatgta ggcgccgagc atgtagatct
      481 cgccgtgggc gaagttgatc agctcgagga tgccgtagac catggtgtag ccgagcgcca
      541 ccagggcgta cacgccgcca agggtgaggc cgttgatgag ttgctggagg aacatcgcgg
      601 ggtgagcgag gggcgcgacc taactcggtt tccagaattg ttcgaagcgt ccatgcttga
      661 cgatccagac gatgtacggc gcctggagga cgtccccctt ggcgtcgaag cgaatctcgc
      721 cctgcgcgcc ctggtggggt gtcccgcgga tgacctcggc gagtttcaga ccgtcggtcg
      781 tgccggcctg cgcgatcgcc gacaagagga tgttggcggc gtcgtacgca tagatcgagt
      841 aagggccgtg cgggccgaac cgttcgtggt acttcgtgag aaacgcctga gactgtggga
      901 gatcggccgg gtcgggtccg aaggtcaggt acgtgccgtc cgccgcgggc cccgcaatct
      961 cgaggaactt ttcatcgtac gtgccgtcgc cggagatgaa cggcgcggcg agccccagct
     1021 cgcgcgcctg ccggaccagg agcgccgcct cgggatagat gccgccgaag aagatgacat
     1081 cgggtttggc gcccttgacg gtggtcagca ccgcgcgaaa gtccttgtcc ccttgggtga
     1141 ttccgctgcc gaagacgacg cgcgggccgc cgccgagcgc ccgctgaaac tcctcggcca
     1201 ggccctggcc gtacgtggtc ttgtcgtgca gcacggccac cgccgcgccg ggaaagcgct
     1261 cgcgcacgaa gcgggcggcg agcggcccct gctggtcgtc gcgcccgcag acgcggaaga
     1321 tcgtggggaa gccctgctcg gtcaggcgcg ggttggtcga ggccggcgtg atcatcacga
     1381 tctttgcttg gttgtacacg cccgacgccg ggatcgaggc gctggagttc cagtggccga
     1441 tcacgccgac cacgcccgag ttgacgaact tgttggcgac cgcgacggcc tgcttgggat
     1501 cgtgctggtc gtcctccacc tgcacgacga tgcgcttgcc gagcacgccg cccctggcgt
     1561 tccactcatc caccgccaac tccacaccgt ggcgcaggtc gatgcccatc ttgctctggt
     1621 cgccggtcat cggcccggcg gcgccgatct tgatctcgtt ggaaaccttc ggcgcgcagg
     1681 caacaatgaa cgcgatactg agcgcggccg acacgccacc gatgacgcgg ccccggaacc
     1741 tcgaagtgag aagtgatctc gacatggcac tcccgtcaga gtggatggag aaaaattgcg
     1801 cctagattac acagggcgga cgaatggtgt caatcggtgg ctgcgtgccg agacgggcgt
     1861 tggccgtgac ggagccgac
//
LOCUS       DBNGWZ010000291         1648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2802716, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000291 DBNGWZ010000000
VERSION     DBNGWZ010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2802716"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="AC16CX_08505"
     CDS             complement(<1..1022)
                     /locus_tag="AC16CX_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012588.1"
                     /translation="MNTAATTAAATPRSFKDVWLISSGHAFTHWYTATFYLLLPLIGK
                     ELGLSYTEIGFIMTTQHVVGAISNLPGGMVVDMVGKKGYLMATSLFWVGFPYALMSLT
                     HSYWPLLVCVTLVGIGNNLWHPAAITTLAYRYPDRKGLVISFHGMGGNVADGVAPLVV
                     GALLAWFSWRTVVVINVVPGLVMAALILIMLGAFTMSSSHDHVNAAGKSRGVKNYLKD
                     FASLLKNRALMLVSIGSAFRTMTQTGLLTFLPVYLAYELEYSPFIVGVCMSAMQVAGF
                     AASPIGGHLSDRMGRKRIVMSSMVLTCVMIVFMALAGKSLAFVLFIALVGFFLYAMRP
                     VLQAWAV"
     gene            1233..>1648
                     /locus_tag="AC16CX_08510"
     CDS             1233..>1648
                     /locus_tag="AC16CX_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3012589.1"
                     /translation="MRTDLERFFNPRAIAIIGASQDLDTISGQPLKFLKSHGYKGRLY
                     PVNPRYQEVAEVKCYPSLADVPEVPDLALVLVNAARVADILRQCGAKGVPYVIIFTSG
                     FSEMGGEGVRLQQELTVIAREHDIGVIGPNCQGMINV"
BASE COUNT          331 a          528 c          515 g          274 t
ORIGIN      
        1 acggcccacg cctgcagcac cgggcgcatg gcgtagagga agaagccgac cagggcgatg
       61 aacagcacga aagcgaggct ctttccggca agcgccatga acacgatcat cacgcacgtc
      121 aggaccatgc tcgacatgac gatgcgcttg cggcccatcc ggtcggatag atggccgccg
      181 atcgggctcg cggcgaagcc cgcgacctgc atcgccgaca tgcagacccc gacgataaac
      241 ggcgaatact cgagctcgta cgcgaggtac accgggagga aagtgagcag tccggtttgc
      301 gtcatcgtgc ggaacgccga gccgatcgac accagcatca gcgcgcggtt cttgagcagg
      361 ctcgcgaaat ccttcaggta attcttcacg ccgcgcgact tgcccgcggc gttgacatgg
      421 tcgtgcgacg agctcatcgt gaacgcgccc agcatgatca ggatcaacgc ggccatcacc
      481 agacccggca cgacgttgat cacgaccacc gtgcgccagc tgaaccaggc gagcaatgcg
      541 cccaccacga ggggcgcgac gccgtcggcc acgtttccgc ccatgccgtg gaaggagatc
      601 accaggccct tgcggtcggg atagcggtag gcgagcgtcg tgatagcggc cgggtgccag
      661 aggttgttgc cgatgccgac cagcgtcacg cacacgagca gcggccagta gctgtgcgtg
      721 aggctcatca gcgcgtacgg gaagccgacc cagaacagcg atgtcgccat caggtaaccc
      781 ttcttgccga ccatgtccac gaccatgccg ccggggagat tcgatatcgc gccgacgacg
      841 tgctgggtcg tcatgatgaa cccgatctcg gtgtagctga ggccgagctc cttgccgatc
      901 agcggcagca gcagataaaa cgtcgcggtg taccagtgcg tgaacgcatg gcccgaggaa
      961 atcagccaca cgtccttgaa cgagcgtgga gttgcggcgg cggtggtggc tgcggtgttc
     1021 acgatacggt ctcggcgttt cgcacggcac gcgcatgata gcattcgcat ccccgcccca
     1081 ccaagcgcgc tcgtcgtata atttccacgt taaatccaga cgcttcgcgc cggtttaact
     1141 tcgcgcgttc gaaccgacga attgatcgcg gttcaacgcg cggtttccac gataacaact
     1201 tttcggacgg cgctggtgtc ccgagtcgct agatgcgcac cgacctcgaa cgcttcttca
     1261 atccgcgcgc gattgccatc atcggcgcgt cgcaggacct cgacaccatc agcggccagc
     1321 cgctgaaatt cctcaagagc cacggctaca agggcaggct ctatccggtg aacccgcgct
     1381 accaggaagt ggcggaagtc aaatgttatc cgtcgctcgc cgatgtgccg gaggtgccgg
     1441 acctcgcgtt ggtactggtg aacgccgcgc gcgttgccga catcctgcgc cagtgcggcg
     1501 cgaagggcgt gccgtacgtg atcatcttca cctccggctt ctccgagatg ggcggcgagg
     1561 gcgtcaggct gcagcaggag cttaccgtca tcgcgcgcga gcacgacatc ggcgtgatcg
     1621 gccccaactg ccagggcatg atcaacgt
//
LOCUS       DBNGWZ010000292         1974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3181223, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000292 DBNGWZ010000000
VERSION     DBNGWZ010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3181223"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..852)
                     /locus_tag="AC16CX_08515"
     CDS             complement(25..852)
                     /locus_tag="AC16CX_08515"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3012590.1"
                     /translation="MVSRNPEVTAQPAATVTLVRDAPRGLEVLMLQRSHSLAFMPGAH
                     VFPGGALDATDESPDLYSLCVGLDDATASRILGVERGGLAYWIAAIREAFEEAGILLA
                     YDASGSIVSLDEAAAQRYIAHRRALDRRHGDFAAFARGEGLRLAADRLLYFGHWITPA
                     SAPRRYDTRFFLAVAPEGQQAWHDNREAIAHAWVRPQEALDLCAREMLNIRFPTVKTL
                     EQFVGCSTVAELISAVTSSNRVQALQPRVTRDGRHVLPGDPGYEEAGVPEGRGTWKP"
     gene            complement(894..1316)
                     /locus_tag="AC16CX_08520"
     CDS             complement(894..1316)
                     /locus_tag="AC16CX_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR3012591.1"
                     /translation="MNAAPVTIRVNRAFGPVVEGEAVLSDEGFSPRYDLDRWSGVITK
                     PGHKLEGVNIRGKILFFPTAKGGIAAGWAFYDIKGKGIAPKALIFGVANPVMVQGAVF
                     ANLTITEGWSRNPRECLRTGDTVRVDPGAKIIQLLKRA"
     gene            complement(1313..>1974)
                     /locus_tag="AC16CX_08525"
     CDS             complement(1313..>1974)
                     /locus_tag="AC16CX_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277762492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR3012592.1"
                     /translation="YAEWGALGKLVGESHQNYFAVPVFTGVRRAPAADELKHLGASLA
                     SYGSMAMYKMVGVTPEAPALEAALGGNKPTDRMNITRSDIERVFAGCRNPDGSCNFIV
                     FSGPQLSLLEMKRLADLFDGRRVHAGTTVFVTTSNSVLSAARALGYAQTLEAAGVTIL
                     EGVCVYILQNLTRMRAANGWTNMISNSAKIVNTITAHKFNTILRRTRECVEIACTGVF
                     R"
BASE COUNT          334 a          675 c          626 g          339 t
ORIGIN      
        1 tcagccgcgt gagcgtgccg gggactatgg tttccacgtg cccctcccct cgggaacacc
       61 agcctcctcg tagcccggat cgccgggaag tacatgcctg ccgtcccgtg taacccgcgg
      121 ctgcagcgct tggactcgat tcgaagaggt caccgccgag atcagctccg cgaccgtcga
      181 gcaacccacg aattgctcga gcgtcttgac ggtcggaaag cgaatgttga gcatttcgcg
      241 cgcgcacagg tcgagcgctt cctgcggccg cacccaggcg tgcgcgatgg cctcgcgatt
      301 gtcgtgccat gcctgctgtc cctccggcgc gaccgcgagg aaaaaacgcg tgtcgtagcg
      361 gcgcggcgcg ctggccggcg tgatccagtg gccaaagtag agcaaccggt cggcggcaag
      421 ccgcaggccc tcgccgcgcg cgaaggcggc gaaatcaccg tgccttctgt cgagggcgcg
      481 ccgatgcgcg atgtagcgtt gtgcggcagc ctcgtccagg gacacaatgc taccgctggc
      541 gtcgtacgca aggaggattc ccgcctcctc gaatgcctcc cggatagcgg cgatccagta
      601 cgcaagcccg ccacgctcca caccaagaat ccggctcgcg gtggcgtcgt cgagtccgac
      661 gcacagcgag tacaggtccg gagactcatc ggtcgcatcg agcgcgccgc ccggaaacac
      721 gtgcgcgccg ggcatgaacg cgaggctgtg gctgcgctgc agcatcagaa cttccagccc
      781 gcgcggcgca tcgcgcacca gtgtaacggt cgcggcaggc tgtgcggtca cttccgggtt
      841 acgactcacc attcaccatt caccattcac cattcacgat tcacgctcaa gagtcaggcg
      901 cgtttcagca attggatgat tttcgccccg ggatccacgc gcacggtgtc accggtgcgg
      961 aggcattcgc gcggattccg cgaccagcct tcggtgatcg tcaggttcgc gaacaccgcg
     1021 ccctgcacca tcaccggatt ggcgacgccg aagatcagcg ccttgggcgc aatgcccttc
     1081 cccttgatgt cgtagaacgc ccagccggcc gcgatgccgc ccttggcggt cggaaagaac
     1141 aggatcttgc cgcggatgtt gacgccctcg agcttgtgcc cgggcttggt gatgactccg
     1201 ctccagcggt cgaggtcgta gcgcgggctg aagccctcgt cggacagcac cgcctcgccc
     1261 tccaccacgg gaccgaaggc gcggttcacc cgaatcgtga ccggagccgc gttcaccgga
     1321 acaccccggt gcaggcgatc tccacgcact cgcgcgtgcg ccgcaggatg gtgttgaact
     1381 tgtgggcggt gatcgtgttc acgatcttcg cggagttcga gatcatgttg gtccagccgt
     1441 tcgcggcgcg catccgcgtg agattttgca ggatgtacac gcacacgcct tcgaggatgg
     1501 taactccagc cgcttctagc gtctgcgcgt agcccagcgc ccgcgccgcc gacaatacgc
     1561 tgttgctcgt ggtcacgaac accgtcgtgc ccgcatgcac gcgcctgccg tcgaacaggt
     1621 ccgccagccg cttcatctcg agcagcgaca gctgcggccc ggagaatacg atgaaattgc
     1681 aactgccgtc ggggttgcgg cagcccgcga acacgcgctc gatgtcggag cgggtgatat
     1741 tcatgcggtc cgtcggcttg ttcccgccga gagcggcttc cagcgccggc gcttcgggcg
     1801 tgacgccgac cattttatac atcgccatcg agccgtagct cgcgagcgag gcgcccaggt
     1861 gcttcagctc atcggcggcg ggcgcgcgcc gcaccccggt gaataccggc acggcgaaat
     1921 agttctggtg cgactcgccg acgagtttac ccagcgcgcc ccactccgcg tagt
//
LOCUS       DBNGWZ010000293         7509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1039797, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000293 DBNGWZ010000000
VERSION     DBNGWZ010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7509
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1039797"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1247)
                     /locus_tag="AC16CX_08530"
     CDS             complement(141..1247)
                     /locus_tag="AC16CX_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3012593.1"
                     /translation="MSDGKSRGPLPKERAIVGCLLGTAAGDALGLACEGLSRGRQLRM
                     FPELAGYKLLPFGKGMCSDDTEHTCMLAQSLIETAGYSDADTMGKRFASNFGWRLRFW
                     LLGLPAGIGLATLRAILKLWIGFPARWSGVFSAGNGPAMRVALIGVCYGDDVPRMRTL
                     VRAATRITHTDPKAEHGALAVAFAAHLAATGKHITPEDYARELRQLLRDDGRELLGLV
                     DDVARSVGRKETAADYATRIGCATGVTGYMLHTVPASLHVWLAHQSDYRNAITAIVRL
                     GGDTDTAAAIVGAIIGARVGKEGIPPEWLRDLWEWPRTRAWMEQLGVKLAERCKDHAN
                     GGAIPLNWLTLLLRNVLFIPLVLAHGFRRLLPPY"
     gene            complement(1265..1909)
                     /gene="maiA"
                     /locus_tag="AC16CX_08535"
     CDS             complement(1265..1909)
                     /gene="maiA"
                     /locus_tag="AC16CX_08535"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR3012594.1"
                     /translation="MKLYSYFRSSAAYRVRIALNLKGLAYEYLTVHLTRGGGEQFAPA
                     FRTLNPQALVPVLQDGERVLAQSLAIIEYLDEVHPRPPLLPRDPASRARVRALALAIA
                     CDIHPLNNLRVLNYLTGPLALGEDAKRTWYHHWIAEGLWALEAKLAGDRETGKFCHGD
                     APGLADCCLLPQIFNARRFKCDLSGYPTLLAIEKNCQALEAFQRAAPDQQPDAE"
     gene            complement(1989..3032)
                     /gene="gtdA"
                     /locus_tag="AC16CX_08540"
     CDS             complement(1989..3032)
                     /gene="gtdA"
                     /locus_tag="AC16CX_08540"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HIR3012595.1"
                     /translation="MSAKPQPNLKRREFYDRISRHDLAPLWEVLGTLVPPSPATPCVP
                     ALWKYREVRSYLMESGELISAAEAERRVLILENPGLRGASAITHTLYAGLQLILPGEV
                     APSHRHTQTALRFIIEGTGAYTAVDGERVTMHPGDFIITPSWTWHDHGNPGNEPVVWL
                     DGLDCPMVAYFDAGFMERYPEATQPVIRAEGDSQARYGANMLPLDYAPRSLTTPVFCY
                     PYGRTRAALERLRRDGDWHPCHGLKLQYSNPASGGYPMPTIGAFVQLLPKGYRGQPYR
                     STDGTVYCAVEGRGRTRIDGKAFEWREQDVFVAPSWSRVQHEADADAVLFSFSDRPAQ
                     KALGLWHEQAPCE"
     gene            3169..4632
                     /locus_tag="AC16CX_08545"
     CDS             3169..4632
                     /locus_tag="AC16CX_08545"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3012596.1"
                     /translation="MTKPKLYNEYERPHADLREFIARIERAGELLRIPGAHWNLEMGT
                     LAEIVNQRSPTNSPAILFDNVPGYPAASRVLSGATNSMKRLAITMGFPVPTHPLDVVR
                     AYRDRMKSYRPIPPQVVKKGPVLENVMRDEEVDLLKFPVPLLHELDGGRYIGTDDVVI
                     MRDPEDNWVNCGTYRVMVHDARRAGVWMSPGKHGRQIREKYFKLGKPCPVLVSCGHDP
                     LLFLAGGNELRFGLSEYDYAGGHRGQPFEVIESELHGLPMPAHAEFVLEGEMMPGDVA
                     PEGPFGEFTGYYAGGQSEQPVIRVRRVYYRSDPILTMATPMRPPSDFSYSKCVMKAGM
                     IWDEVERAGLSNVKGVWCHEAGGARMFNVIAIKQAYAGHSKQAGMAAASCQSGSYLGR
                     FVVVVDEDIDPTDLFDVLWAMCTRCDPAEDIDIIRKMWSGPLDPRIPRGTAWNSRAVI
                     DACRPFEMIKEFPPVARESRELRAKIETKFHDILSRL"
     gene            4672..6114
                     /locus_tag="AC16CX_08550"
     CDS             4672..6114
                     /locus_tag="AC16CX_08550"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIR3012597.1"
                     /translation="MNLVSSNIRPAPDKVLVEIADYVDRYPVKSALALETARLALIDS
                     LGCGFEALSYPACTKLLGPVVPGTIVPNGARVPGTPYVLDPERATLNIGALVRWLDFN
                     DAFYGETVIHPSDTFAGILAVADWLSRSRVAEGKKPLLMREVLDAAVKTYEIMGGLAL
                     ENGFTAAGLDHTMLVKIATAPVVTKLLGGTHEEIVNALSQAWVDGHALATFRRKPNTG
                     PRKSWAAGDAASRGVRLALMAVKGEIGCPSALTAKTWGFYHVLFRNRPFRFQRPFGTY
                     VMENVLFKIPYPTAFHGQSGVEAAIKLHPLVKDRLDDIKRVEVRCHNSTMVILDKTGP
                     LHNPADRDHCMQYMMAVGMIYGTMTAEHYEDHIAADPRIDALRAKMHLAESRQYEREY
                     HDPAKRTNANSIQVFFKDGTRTPLSEVLYPLGHRRRRKEGIPVLVKKFETNVGRVFAH
                     KQRDAILRVCLDQKRLEAMPVNEFMDLLAA"
     gene            6236..6607
                     /locus_tag="AC16CX_08555"
     CDS             6236..6607
                     /locus_tag="AC16CX_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012598.1"
                     /translation="MKVTVAIGLLALVWSGAAGAQQPLVGKWSGTLADQARPDRTGTI
                     EMEITNADGGKLKGTMTLYPYKGRGCPGRYQFEGTYQDNKIEFNSTGGMSDCNVKYQL
                     VVEGNQLTGGTGDTSVKLRKK"
     gene            6804..>7509
                     /locus_tag="AC16CX_08560"
     CDS             6804..>7509
                     /locus_tag="AC16CX_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3012599.1"
                     /translation="MEQRKRLRAVFAGTRCISPASVFDPLSARVAESVGYEIGMLAGS
                     VASNTTLAAPDLIVLTLTEFAGQIRRIMRASKLSLIVDADHGYGNALNVMRTVEELEH
                     AGVSGMSIEDTALPARFGQPEGADELISLEEMVGKLKAAVAARRDPSTVILGRTAALK
                     VEGTEGAVKRAKAFAATGVDAIFVVGVESADQVKAVHDAAKLPIMVGSAPASIKREDF
                     AAAGARVLLQGHQPVPA"
BASE COUNT         1362 a         2395 c         2514 g         1238 t
ORIGIN      
        1 gcacgccgga agagctcgct gcccagatca agcgcgagat cgtcaagtac gcggaagtca
       61 tccgcaaggg cagcatcaag ctgcagtgag gcggaaggca aagggagctg cttagcaaga
      121 ttttccttca tccttgggtt ctagtagggc ggaagcagcc gccggaatcc gtgtgccagc
      181 acgagcggga tgaagagcac gttgcgcagc aggagcgtca gccagttaag cgggatggcg
      241 ccgccgttcg cgtgatcctt gcagcgttcc gcgagcttga cgccgagttg ttccatccac
      301 gcccgggtgc gcggccattc ccacagatcg cgcagccact ccggggggat gccttccttg
      361 ccgacgcgcg cgccgatgat ggcgcccacg atggccgccg cggtatcggt gtcgcctcca
      421 aggcggacga tcgcagtgat cgcattccgg tagtcgcttt gatgcgcgag ccagacgtgg
      481 agggacgcgg ggacagtgtg tagcatgtag ccggtcacgc cggtcgcgca tccgatgcgg
      541 gttgcatagt cggcggcggt ctccttccgg cccacgctgc gcgcaacatc atccaccaat
      601 cctagcagct cgcgcccatc gtcacgcagc agctggcgga gctcgcgcgc gtagtcctcg
      661 ggcgtgatgt gcttgccggt cgcggcgagg tgagcggcga acgccaccgc cagcgcgccg
      721 tgctcggcct tgggatcggt gtgggtgatg cgcgtcgcgg cgcgcaccag tgtgcgcatc
      781 cggggaacat cgtctccgta gcacacgccg atcagagcga cccgcatcgc gggcccgttg
      841 cccgccgaga acacgccgct ccagcgcgct gggaagccga tccacagctt gaggatcgcg
      901 cgcagcgtgg caagcccgat gcccgccggc agccccagca gccagaagcg caaccgccag
      961 ccgaagttgg aagcgaacct cttccccatg gtgtccgcgt cactatagcc ggcggtttcg
     1021 atcagggact gcgccagcat gcaggtgtgc tcggtgtcgt cggagcacat gcccttgccg
     1081 aaggggagga gcttgtagcc ggcgagctcg ggaaacatcc tcagttgccg gccgcgactc
     1141 agaccctcac acgcgagccc cagcgcatca cccgccgcgg tgccgaggag gcaacccacg
     1201 atcgcgcgct ctttcggcag cggcccgcgc gatttgccgt cgctcatgcg cgggctagtc
     1261 cccgtcattc agcgtccggc tgctggtcgg gcgcggcgcg ttgaaacgct tcgagcgcct
     1321 ggcagttttt ttcgatggcg aggagggtag gatagccgga cagatcgcat ttgaaccgcc
     1381 gtgcgttgaa gatctgcggc agcaggcagc agtcggcaag acccggtgcg tcgccatggc
     1441 agaactttcc cgtctcgcga tcgccggcaa gcttggcctc gagtgcccac aatccttccg
     1501 ctatccagtg gtgataccac gtgcgcttcg cgtcctcacc gagggccagc ggcccggtga
     1561 ggtagttcag caccctcaga ttgttcaaag ggtggatgtc gcaggcgatg gccagcgcca
     1621 gagcgcgcac ccgcgcccgg gatgccgggt cccgcggcag gagggggggc cgcggatgaa
     1681 cttcgtcgag gtactcgatg atggcgagcg actgcgcgag cacgcgctcg ccgtcctgaa
     1741 gcaccggcac cagcgcctgc ggattcagcg tgcggaatgc cggcgcgaac tgctccccgc
     1801 cgccgcgcgt gagatgcacc gtcaggtatt cgtaagcgag tcctttgaga ttcaacgcaa
     1861 tgcgaacgcg gtaggcggcg gagctgcgga agtaggaata gagcttcatg acaccatgag
     1921 cagtaagcgg taagcggtca gcggaaacgg ctgatggaaa ctaccgctta ccatttactg
     1981 cttaccgctt actcgcaagg cgcttgctcg tgccacaggc cgagcgcctt ctgcgctggc
     2041 cggtcggaga agctgaacag cacggcgtcc gcgtccgcct cgtgctgcac gcggctccac
     2101 gacggcgcga cgaacacgtc ctgttcccgc cattcgaacg cttttccgtc gatccgggtg
     2161 cggccgcgcc cctcgaccgc gcagtacacg gtgccgtcgg tcgagcggta gggctgcccg
     2221 cgatatccct tgggcagcag ctggacgaac gcgccgatgg tgggcatcgg gtagccgccg
     2281 ctcgcgggat tggagtactg gagcttgaga ccgtggcacg gatgccagtc gccgtcgcgg
     2341 cgcaaccgct cgagcgccgc gcgcgtgcgc ccgtagggat agcagaacac cggcgtcgtg
     2401 agcgatcgag gcgcgtaatc gagcggcagc atgtttgcgc cgtaacgcgc ctgggagtcg
     2461 ccttccgccc ggatcacggg ctgcgtcgct tccgggtagc gctccatgaa acccgcgtcg
     2521 aaatacgcga ccatcgggca atccaggccg tcgagccata ccaccggctc gttgccggga
     2581 ttgccgtgat cgtgccaggt ccacgagggc gtgatgatga aatcgccggg gtgcatcgtc
     2641 acgcgctcgc cgtccaccgc ggtgtacgcc cccgtgccct cgatgatgaa gcgcaacgcg
     2701 gtctgggtat gccggtggct cggcgcgacc tcgccgggca ggatcagctg cagcccggcg
     2761 tagagcgtat gcgtgatcgc cgaggctccg cgcagaccgg gattttccag gatcagcacg
     2821 cgccgctcgg cctccgccgc cgagatcagc tcgcccgatt ccatgaggta ggagcggact
     2881 tcgcggtatt tccacagcgc gggcacgcac ggcgtggcgg gtgagggcgg caccagcgtg
     2941 ccgagcacct cccacagcgg cgcgaggtcg tggcgcgaga tgcggtcata gaactcgcgg
     3001 cgcttcaggt tgggctgcgg cttggctgac atgaccctat tttataccgg acgaggcaga
     3061 attctgcccc gacagcgcta aaatcacagt cttggtctca agcgctggac gcgcgttatc
     3121 gccgaggcga agattgaggc tgtccgacca gaggtctccg tgaacgaaat gacgaagccg
     3181 aagctttaca acgaatacga gcgacctcac gctgacttgc gcgaattcat cgcccggatc
     3241 gagcgcgccg gcgagctcct gcgcatcccg ggcgcgcatt ggaacctcga aatgggaacc
     3301 cttgccgaga tcgtgaacca gcgcagcccg accaattcgc cggcgatcct gttcgataac
     3361 gtgcccggat atcccgccgc ctcgcgcgtg ctgtccggag cgaccaactc gatgaagcgt
     3421 ctcgcgatca cgatgggctt tccggtaccg acgcacccgc tcgacgtcgt gcgcgcctac
     3481 cgcgatcgca tgaaaagcta ccgcccgatc ccgccgcagg tggtcaagaa ggggcccgtg
     3541 ctcgaaaacg tcatgcgcga cgaggaggtt gatctcctca agtttcccgt gccgctgctg
     3601 catgaactcg atggcggccg ctacatcggc accgacgacg tggtaatcat gcgggacccc
     3661 gaggacaact gggtcaactg cggcacctac cgggtgatgg ttcacgacgc caggcgcgcc
     3721 ggcgtgtgga tgtcgcccgg caagcacgga cgccagatcc gcgaaaagta cttcaagctc
     3781 ggcaagccct gtccggtgct cgtttcctgc ggccacgatc cgctgttgtt cctcgccggc
     3841 ggcaatgaac tgcgcttcgg gctgtcggag tacgactacg ccggcgggca ccgcggccag
     3901 ccgttcgaag tgatcgaaag cgagctccac ggcctgccga tgccggcgca cgccgaattc
     3961 gtgctcgagg gcgaaatgat gcccggcgac gttgccccgg aggggccgtt cggggaattt
     4021 accggctatt acgcgggtgg ccagagcgag cagccggtga tccgggttcg gcgcgtctat
     4081 taccgcagcg acccgatact cacgatggcg acgccgatgc gtccgccctc ggacttctcc
     4141 tacagcaagt gtgtgatgaa ggccggcatg atctgggacg aggtcgagcg cgcagggctc
     4201 tcgaacgtga aaggggtctg gtgtcacgag gcgggcggcg cgcgcatgtt caacgtcatc
     4261 gccatcaagc aggcctacgc cgggcattca aaacaggcgg gcatggctgc ggcaagctgc
     4321 cagtcgggtt cctatctcgg ccgctttgtc gtggtggtgg acgaggacat tgatcccacc
     4381 gacctgttcg acgtgctgtg ggcgatgtgc acgcgctgcg atcccgcgga agacatcgac
     4441 atcatccgca agatgtggag cgggccgctc gatccgcgca tcccgcgcgg caccgcctgg
     4501 aactcgcgcg cggtgatcga tgcctgccgg ccgttcgaga tgatcaagga gtttccgcca
     4561 gtggcgcgcg agagccgcga actgcgggcg aaaatcgaaa ccaagttcca cgatatcctc
     4621 tcccggctct aggccgtctc tcacggcacc gttcgcgcat ctggagtcat catgaactta
     4681 gtgtcctcaa acattcgtcc cgcgcccgac aaggtgctcg tcgagatcgc cgattacgtt
     4741 gaccgttacc cggtgaaaag cgcgctcgcg cttgagaccg cgcggctcgc gctgatcgac
     4801 agcctcggtt gcggcttcga ggcgctgtcc tatcccgcct gcaccaagct gctcgggccg
     4861 gtcgtgccgg ggaccatcgt cccgaacggc gcgcgcgtgc cgggcacgcc atacgtgctg
     4921 gacccggagc gggcgacgct gaacatcggg gcgctcgtgc gctggctcga cttcaacgac
     4981 gcgttctacg gcgagaccgt catccacccc tccgacacct tcgccggcat actggcggtg
     5041 gcggactggc tttcccgctc gcgcgtggcg gaagggaaaa agccgctcct catgcgcgag
     5101 gtgctggacg cggcggtcaa gacctacgag atcatgggcg ggcttgcgct cgagaacggc
     5161 ttcaccgccg ccgggctcga ccacaccatg cttgtgaaga tcgccacggc gccggtggtg
     5221 acgaagcttc tcggcggcac gcacgaggag atcgtcaacg cgctttccca ggcctgggtg
     5281 gacgggcacg cgcttgcaac cttccgcagg aagcccaaca cgggcccgcg caagtcgtgg
     5341 gcggcgggcg atgccgcgag ccgcggcgtg cggctcgcgt tgatggcggt gaaaggggag
     5401 atcggctgtc cctcggcgct gacggcgaag acatggggtt tctaccatgt gctgttcagg
     5461 aaccggccgt tcaggttcca gcgcccgttc ggcacctacg tcatggagaa cgtgctgttc
     5521 aagattccat accccacggc gttccacggg cagagcggcg tggaggcggc gatcaagctc
     5581 catccgctgg tgaaggaccg tctcgacgac atcaagcgcg tggaagtgcg gtgccacaac
     5641 tcgaccatgg tgatcctcga caagaccggt ccgctgcaca accccgcgga ccgcgaccac
     5701 tgcatgcagt acatgatggc ggtcggcatg atctacggaa cgatgacggc ggagcattac
     5761 gaggatcaca tcgcggcgga cccgcgcatc gacgcgctgc gcgccaagat gcacctggcc
     5821 gaatccaggc agtacgagcg ggaataccac gatcccgcga agcgcaccaa cgccaactcg
     5881 atccaggttt tcttcaagga cggcaccagg accccgctgt cggaggtgct ctatcccctc
     5941 ggccaccgcc gccggcgcaa ggagggcatc ccggtgctgg tgaagaagtt cgagaccaac
     6001 gtcgggcgcg tgttcgccca caagcagcgc gacgcgatcc tccgggtctg cctcgaccag
     6061 aagcggctgg aggcgatgcc ggtgaacgag ttcatggatc tgctcgccgc gtgaagaagt
     6121 cgcgacaagt tgatatcatg atttccgcga caacggaatc ccgctgtttc aggtcggaga
     6181 aaaataccgg gtcgcgatcc gtgtcgtcat aaaccagatt cctggaggag gctctatgaa
     6241 ggtcacagtc gcaattggtc tgcttgctct ggtctggtcc ggtgcagcgg gtgcacaaca
     6301 gcccttggtc ggcaaatgga gcggcacgct ggccgaccag gcgcgcccgg atcgaacggg
     6361 caccatcgag atggagatca ccaacgcgga cggcgggaaa ctgaagggaa cgatgaccct
     6421 gtacccgtac aagggaaggg gctgcccggg cagataccag tttgaaggta cctatcagga
     6481 caacaagatt gaattcaatt ccaccggcgg gatgtccgac tgcaacgtga agtatcaatt
     6541 ggtggtcgag ggaaaccaac tgacgggcgg cacgggcgat accagcgtca agctcaggaa
     6601 gaagtaattc acggttcgtt gcgtcctgaa gtcatccggg ttcgatcacc ggtcggatcg
     6661 ccgggcggtg gcgacgcggt gtcagtgccg tgcatcaggc gggccagccg cgagcaaccg
     6721 gctcgcgatg ggtggtcacc ggaatgcgcg cgctgccgac agcccgggaa tcgcatcgat
     6781 atagcggagt ggtcatgagc cctatggaac aacgaaaaag gctgcgcgcg gtcttcgccg
     6841 ggacgaggtg catatcgccg gcgagcgtgt tcgacccgtt gtcggcgcgg gtcgccgagt
     6901 cggtcggcta cgagatcggc atgctggcgg gatcggtcgc gtcgaacacg acgctcgccg
     6961 cgcccgatct catcgtgctg acgctcaccg agttcgctgg ccagatccgg cgcatcatgc
     7021 gcgcaagcaa gctgtcgctg atcgtggacg cggaccacgg ctacggcaac gctctcaacg
     7081 tcatgcggac ggtcgaggaa ctcgaacatg ccggagtttc aggcatgagc atcgaggaca
     7141 ccgcgttgcc cgcgcgcttc ggccagcccg aaggcgccga cgagctgatc tcgctcgagg
     7201 agatggtggg caagctcaag gccgccgtcg ccgcgcgccg cgatccttcg accgtcatac
     7261 tcgggcgcac agcggcgctg aaggtggaag ggacggaagg cgccgtgaag cgggcgaaag
     7321 ccttcgcggc gaccggtgtg gacgcgatct tcgtcgttgg agtggaatct gcggaccagg
     7381 tcaaggcggt gcacgacgcg gcgaagctcc cgatcatggt cggatcagcg ccggcttcca
     7441 tcaagcgcga ggatttcgcc gccgccggcg cgcgcgtcct gctgcaaggc catcaaccgg
     7501 tcccggccg
//
LOCUS       DBNGWZ010000294         9309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_537396, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000294 DBNGWZ010000000
VERSION     DBNGWZ010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_537396"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..242
                     /gene="rpoZ"
                     /locus_tag="AC16CX_08565"
     CDS             36..242
                     /gene="rpoZ"
                     /locus_tag="AC16CX_08565"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3012600.1"
                     /translation="MARITVDDCLKVIPNRFEMSLAATYRARQITNGAQPMIEASRDK
                     PTVTALRELAAGKYGAEILNKGQT"
     gene            344..2464
                     /locus_tag="AC16CX_08570"
     CDS             344..2464
                     /locus_tag="AC16CX_08570"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3012601.1"
                     /translation="MQAAAAALFKDWSGYLKPEDISQLESAFHFSEQAHEGQFRQSGE
                     PYVSHPLAVANILAQWHLDPQALTAALLHDVMEDTSVTKTEISRNFGRPVAELVDGVS
                     KLDKIEFESHAEAQAENFRKMLLAMARDVRVILIKLADRLHNMRTLDAVAPDKRRRIA
                     RETLEIYAPIANRLGLNSIYQELEDLSLKHLYPDRYRVLAKAVKAARGNRREVVGKVL
                     DAIRRKLKNTGVEAHALGREKHLCSIYRKMREKHLTFAQVLDVFGFRIIVNDVPACYV
                     ALGALHGLYKPIPGKFKDYIAIPKANGYQSLHTTLFGPFGTPMEVQIRTQEMHKIAEA
                     GVASHWLYKGADTSLSDLQKKTHQWLQSLLEIQSQSGDSAEFLEHLKVDLFPDEVYVF
                     TPKGKILSLPRGATAVDFAYAVHTDIGNRCVAAKINQELMPLRTDLKNGDRVEIITAA
                     HAKPNPLWLNFVTTGKARSRIRHFLKTMHFQESAKLGERLLNQALAALGAAPADIKEA
                     QWDKFVRDAGAKSRQEVLADVGLGKRLAAVVARRLLSRSEPAGPHEAPAPGSVIIRGS
                     EGMAVQFAHCCRPIPGDPIIGLISKGQGMVIHTHDCPVITKARHDPDRVLDVAWDPET
                     KKLFEVSIKLVVANQRGVLAKVAAEIAEAGSNIQNVAVDPDDGSSYTNMFFTLQVSNR
                     LHLARIMRGLRRIPEVARLSRVKG"
     gene            complement(2572..2838)
                     /locus_tag="AC16CX_08575"
     CDS             complement(2572..2838)
                     /locus_tag="AC16CX_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012602.1"
                     /translation="MKDGNWKRACKRGLLLALLAGCAQLQPAPPPASRVNLTGYSPSF
                     KQGYADGCESAGTRSQRRQEGRYKTEADYMMGWNDGFSACQRRR"
     gene            complement(2835..3794)
                     /locus_tag="AC16CX_08580"
     CDS             complement(2835..3794)
                     /locus_tag="AC16CX_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012603.1"
                     /translation="MSELLSASRGNNRWWWGLRQMTLHIVITLLALGIAFSLPVAAQY
                     ILYQWWPRVVSDANLLLASEVSLAASLVLLFNLWRVASENRLKGKVADTAALVYARNR
                     ANWLTRIRERSLVRKLPAVRDAFILTLTGYDTFADAKSLLRKPLQSAYEIRVMLLNPA
                     ARGAEKRINSLPQEITQQSYIEEVEASIAYLAMLRTLGKSVTLKFYEHEPFWKVVVLG
                     DYLWLQYCHSGFEVKGEPEYVFALNPDNPRRGLFVPFYMYFLEQWGDARHPRYDFDTR
                     ELVYCDDHGREIRRTRFVGGRAHTPALLHPQAAPPTAPTAAHA"
     gene            complement(3837..4136)
                     /locus_tag="AC16CX_08585"
     CDS             complement(3837..4136)
                     /locus_tag="AC16CX_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012604.1"
                     /translation="MNKVSHGRLKRINAEFRGDEAVEIDILEVMLRMGEVARTRIESD
                     TKLVRLALKALDRVIADCCGRTGQRYPTYSMLVKYLDERDPVPQPEPTRVVSMQK"
     gene            complement(4249..5100)
                     /locus_tag="AC16CX_08590"
     CDS             complement(4249..5100)
                     /locus_tag="AC16CX_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012605.1"
                     /translation="MRTVSALLLAGVLSAGNLAAGAQTPDQTNPPTTTTANGRETPAQ
                     TRIVNSFAAFAGSEANAQSLVTGLRQGSEITLTAPGTGGQTGTSTTFTPPTRPMGYGN
                     VRIALALAQEQLAQTGITQPTPEQLRVALMGGTTTEFAGVLQMRADGMGWGQIANSMG
                     VKLGHVLSGRTGQQPSTASSPASSGSASTGADTGTGTGITTATGGTSAAVTTRNRGNS
                     ASAQQTRRPGAGIVTAAGVVAAGASAGPSVQSGGNAAGVVTGAGVAAGSRASATGQAH
                     GKGFVEP"
     gene            complement(5097..5513)
                     /locus_tag="AC16CX_08595"
     CDS             complement(5097..5513)
                     /locus_tag="AC16CX_08595"
                     /inference="COORDINATES: protein motif:HMM:NF023514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HIR3012606.1"
                     /translation="MKSRIALLLVAGAAGLAGCEPLSITAFGVGASAGVTHSMGGITY
                     RTFTAPLPKVKGATLTALNRMGIKVASSSKAEGSELIKANAAGREIEIQLEPLTPNTT
                     RMRTVARNGWFFDSATATEIILQTERAMGNGKGEPS"
     gene            complement(5522..5689)
                     /locus_tag="AC16CX_08600"
     CDS             complement(5522..5689)
                     /locus_tag="AC16CX_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012607.1"
                     /translation="MAGPTRDVVDTWDLSEILAALRQIARDAGEADNAAETETEVVRR
                     ITFALSPGDRQ"
     gene            complement(5714..6310)
                     /locus_tag="AC16CX_08605"
     CDS             complement(5714..6310)
                     /locus_tag="AC16CX_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3012608.1"
                     /translation="MEHTVNTTGTEHTGEKRSGILYPVMLIAAIAVIVFSVAGIATMM
                     GWLPSVQSKSEQPANAPAATKAAPTATKPAASRPRAVASASAACNDCGVVDSISVVEV
                     PGEGTGLGAIGGAVVGGILGNQVGRGRGRTAATVVGAGAGAYAGHEIEKNAKKSASYE
                     IRVRMNDGSIRTFHEHTQPPLSVGQKVRVTEQGIVAAG"
     gene            complement(6376..6876)
                     /locus_tag="AC16CX_08610"
     CDS             complement(6376..6876)
                     /locus_tag="AC16CX_08610"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3012609.1"
                     /translation="MNSKQVGYRAVVSALVASWILAAAAAGASEADGQFNREFKKLDA
                     NGDGYLTLEETRKIRGFDQAFKEADDNRDGRLDADEFVKAQAIHGRMLTGQYVEDSVI
                     TAKIKAALLKDPLVSAVAVGVQTYKGTVLLSGFVDNENQVRRAAEIAAGVKGVIEVKN
                     GLAIKS"
     gene            complement(7282..8634)
                     /locus_tag="AC16CX_08615"
     CDS             complement(7282..8634)
                     /locus_tag="AC16CX_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR3012610.1"
                     /translation="MNAARINILVVDDDPDLLRLMQIRLTAAGYAVSTAESGERALAQ
                     VSVSRPQLVVTDLRMGGMNGMALFEAIQAGHPTLPVIILTAHGTIPDAVAAVQRGVFG
                     YLTKPFDPKALLAEVERALAMAGGAQAAGDASEEWRRGIITRNPAMEDVLAKARMVAE
                     SDASVFIQGDSGTGKELIARAIHQASPRRDHPFVAINCGAIPEQLLESELFGHVKGAF
                     TGAVRDNKGLFQTANRGTLFLDEIGDMPLSLQVKLLRVLQEKKVRAVGSTEAHEVDVR
                     VISATHRNLEAEMAASNFREDLYYRLNVVALALPPLTERREDIPLLANHFLSSLAEKY
                     KKAVNGFAPEALEMMVAASWPGNVRQLYNVVEQSVALATTPIIPVSLAQQAIQNQQAE
                     FASFEEARRRFEREYLTQLLKITEGNVTQAARLAKRNRTEFYKLLGRHQLDPKLFKSR
                     "
     gene            complement(8631..9116)
                     /locus_tag="AC16CX_08620"
     CDS             complement(8631..9116)
                     /locus_tag="AC16CX_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012611.1"
                     /translation="MTWRWLLVFPACAALLAGCAALRGVETQEPAPWVQDGSPQPASN
                     AESLLLYYQHVRKLSGAELAREHETALQAYTGSPSDFSRVRLAMVLSLPNTAFSDEGR
                     ALELIEPVSRNQDGQLRGLAYLLAVHLQERRRLAANAQGLQQKLDALKSLERSMIERK
                     R"
     gene            complement(9113..>9309)
                     /locus_tag="AC16CX_08625"
     CDS             complement(9113..>9309)
                     /locus_tag="AC16CX_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3012612.1"
                     /translation="RTRIFDPFYQGRHAGAGPVRGTGIGLSVVKEYVFAHGGSVEVVD
                     NRQGAHLRVRVPLKRGEAAI"
BASE COUNT         1532 a         3121 c         2971 g         1685 t
ORIGIN      
        1 cgtgatcaac cgcatgaagt aaccgaggta ctgctatggc tcgtatcacc gttgacgatt
       61 gtttgaaagt gattcccaac cgcttcgaaa tgtcgctcgc ggcgacctat cgcgcgcgcc
      121 agatcacgaa cggcgcccag cccatgatcg aggcgagccg cgacaagccc accgtgaccg
      181 cgctgcgtga gctcgccgcc ggcaagtacg gtgcggaaat cctgaacaag ggacagacat
      241 gacatcaagt cgaaaggcgg aaggcggaat gcggaagtag ccggctgacg ccgcctgctg
      301 ccgtttactt tcgcctttcg actttcgcct ttcgccttcg taaatgcagg ccgccgccgc
      361 cgcgttgttc aaggactggt ccggctatct taagccggag gatatctcgc agctcgagtc
      421 ggccttccac ttcagcgagc aggcgcacga aggccagttc cgccaatccg gcgagcccta
      481 cgtctcgcac cccctggcgg tcgccaatat cctcgcgcag tggcacctcg atccccaggc
      541 gctcaccgcc gccctcctcc atgacgtgat ggaggacacc tcggtcacca agaccgagat
      601 ctcgcgcaat ttcggaaggc cggtcgccga gctggtggac ggcgtctcca agctcgacaa
      661 gatcgagttc gagtcgcacg ccgaggcgca ggccgagaac ttccgcaaga tgctgctggc
      721 gatggcgcgc gacgtgcgcg tgatcctgat caagctcgcc gaccggctgc acaacatgag
      781 gacgctcgac gcggtcgcgc ccgacaagcg gcgccgcatc gcccgcgaga cgctcgagat
      841 ctacgccccg atcgcgaacc gcctcggcct gaacagcatc taccaggagc tcgaggacct
      901 ctcgctcaag cacctctacc ccgaccgcta ccgggtgctc gccaaggcgg tgaaagcggc
      961 gcgcggcaac cgccgggagg tggtgggcaa ggtgctggat gcgatccgga gaaagctcaa
     1021 gaacaccggc gtggaggccc acgcgctggg gcgcgagaag cacctctgtt cgatctaccg
     1081 caagatgcgc gagaagcacc tcaccttcgc gcaggtgctc gacgtgttcg gcttccgcat
     1141 catcgtcaac gacgtgccgg cctgctacgt cgcgctgggg gcgctccacg gcctctacaa
     1201 gccgatcccc ggcaagttca aggactacat cgccatcccc aaggcgaacg gctaccagtc
     1261 cctgcacacg acgttgtttg ggcccttcgg cacgcccatg gaagtgcaga tccgcaccca
     1321 ggagatgcac aagatcgcgg aagcgggggt ggcctcgcac tggctctaca agggggcgga
     1381 cacctcgctc tccgacctgc agaagaagac ccaccagtgg ctgcagagcc tgctcgagat
     1441 ccagtcgcag tcgggggact cggccgaatt cctcgagcac ctcaaggtgg acctgttccc
     1501 ggacgaggtc tacgtgttta ccccgaaggg caagatcctg tcgctgccgc gcggggccac
     1561 cgcggtggac ttcgcctacg ccgtgcacac cgacatcggc aaccgctgcg tggcggcgaa
     1621 gatcaaccag gagctgatgc cgctgcggac cgatctcaag aacggcgacc gcgtcgagat
     1681 catcaccgcg gcgcacgcca agcccaatcc cctctggctc aacttcgtca cgaccggcaa
     1741 ggcgcgctcg cgcatccggc acttcctcaa gaccatgcac ttccaggaat cggccaagct
     1801 cggcgagcgc ctgctcaacc aggccctggc cgccctcggg gctgcgcccg ccgacatcaa
     1861 ggaagcgcaa tgggacaagt tcgtgcgcga cgccggggcc aagtcgcgcc aggaggtgct
     1921 cgccgacgtc ggcctcggca aacgtctcgc cgcggtcgtg gcgcgaaggc tgctctcgcg
     1981 cagcgaaccc gccggcccgc acgaggcgcc ggcgcccggc tcggtcatca tccgcggctc
     2041 ggaaggcatg gcggtgcagt tcgcccactg ctgccggccg atcccgggcg accccatcat
     2101 cggcctcatc agcaagggcc agggcatggt gatccacacc cacgactgcc cggtcatcac
     2161 caaggcgcgc cacgatcccg accgcgtgct cgacgtggcg tgggaccccg aaaccaagaa
     2221 gctgttcgag gtcagcatca agctggtggt cgccaaccag cgcggcgtgc tcgccaaggt
     2281 ggcggccgaa atcgccgagg cgggctccaa catccagaac gtggcggtgg accccgatga
     2341 cggcagcagc tacaccaaca tgttcttcac gctgcaggtg tcgaaccgcc tgcacctcgc
     2401 gcgcatcatg cgggggctcc ggcgcatacc cgaggttgcc cgcctgtcac gggtgaaagg
     2461 ctgaacgacc atcggaaatt ccaaattcca atactcaaat ttcaaatacc aaacgcttgg
     2521 tgcttggagt ctggcgcttg gagtttggtg cttggaattt agcgcgcagc gctagcggcg
     2581 gcgctggcag gcgctgaagc cgtcgttcca tcccatcatg taatcggcct cggtcttgta
     2641 gcggccctcc tggcggcgct ggctgcgcgt gccggcgctt tcgcagccgt cggcgtatcc
     2701 ctgcttgaac gacggtgagt agcccgtgag attgacgcgt gacgccggcg gaggcgctgg
     2761 ctgcagctgc gcgcaccccg ccagcagcgc caggagcagt cctcgtttac acgctcgttt
     2821 ccagttcccg tctttcatgc gtgggccgcc gttggggcgg ttgggggcgc agcctgcgga
     2881 tggagcaacg ccggcgtatg cgccctgccg ccgacgaagc gggtgcgacg gatttcgcgg
     2941 ccgtggtcat cgcaatagac cagctcgcgg gtatcgaagt cgtaacgcgg atggcgcgcg
     3001 tcgccccatt gctcgagaaa atacatgtaa aacggcacga acagaccgcg cctgggattg
     3061 tccgggttga gggcgaacac gtattccggc tcgcctttga cctcgaagcc gctgtggcag
     3121 tattgcagcc acaggtagtc gccgagcacc accaccttcc agaacggctc gtgctcgtag
     3181 aacttgagcg tcacgctctt cccgagcgtg cgcagcatcg ccagatacgc gatgctcgcc
     3241 tccacctcct caatgtagga ttgctgcgtg atttcctgcg gaagggagtt gatgcgcttt
     3301 tccgcgcccc gggcggccgg attgagcagc atgacgcgga tctcgtaggc cgattgcagc
     3361 ggtttccgca acaggctttt cgcgtcggca aaggtgtcgt atccggtcaa tgtcagaatg
     3421 aaggcgtcgc gcacggcggg cagcttcctg accaggctgc gttcgcgaat ccgcgtcagc
     3481 cagttcgcgc ggttgcgcgc atagaccagg gcggcggtgt cggcgacctt tcccttgagc
     3541 cggttctcgg aggcgacgcg ccacagattg aacagcagca ccagcgacgc ggccaggcta
     3601 acttccgaag cgagaagcag attggcgtcc gagacgacgc gcggccacca ctggtaaagg
     3661 atgtattggg cggcgaccgg caacgagaac gcgatcccga gagcgagcag cgtgatgaca
     3721 atgtgcaatg tcatctgacg caggccccac caccaccggt tgttgcctct cgatgcggaa
     3781 agaagctcgc tcatgccgtt ctccgcttca ggtccggctc attcgcgcgt cgccggtcat
     3841 ttctgcatgc tgacgacgcg cgtcggctcc ggctgcggca cgggatcccg ctcgtcgaga
     3901 tatttcacga gcatcgagta ggtgggataa cgctggccgg tccggccgca acagtcggcg
     3961 atcacccggt cgagcgcctt gagcgccagg cgcacgagct tggtgtcgct ctcgatgcgc
     4021 gtgcgcgcga cctcgcccat gcgcagcatc acttccagga tgtcgatctc caccgcttcg
     4081 tcgccccgga actccgcgtt gatccgcttg aggcgcccat gactcacctt gttcattgtc
     4141 cccttccttc cctaaaaagt tgcgccggcc gcgtggtcgc ggccggtgat tgctgcgcgc
     4201 ggtatcctga ataccgaggg ggcgcggcga ccccttcacc accggcgctc agggttcaac
     4261 gaaccctttc ccgtgcgcct ggcccgtggc tgaagcgcgg ctgcctgcgg ccacgccggc
     4321 tccggtgacc acgcccgccg cgttcccacc gctttggaca cttggaccag cgctcgcccc
     4381 cgcggcgaca acgccagccg cggtcacgat gccggctccg ggccggcggg tctgttgcgc
     4441 cgatgcgctg ttgccgcggt ttcgcgtcgt gacggcggcg gacgttccgc ccgtcgcggt
     4501 ggtgataccc gttccggtgc cggtgtcagc gccggtcgaa gccgaacccg atgaggccgg
     4561 cgaggacgca gtggacggct gctgaccggt cctgccgctc aggacatgtc cgagttttac
     4621 gcccatgctg ttggcgatct ggccccagcc catcccgtca gcgcgcatct gcagtacgcc
     4681 cgcgaactcg gtggtggtcc cgcccatgag cgcgaccctc agctgttcag gcgtgggctg
     4741 cgtaatgccg gtctgggcga gctgctcctg cgccagcgcc agcgcaatgc ggacgttgcc
     4801 gtagcccatg gggcgggtgg gcggcgtaaa cgtggtgctc gtgccggttt gcccgcctgt
     4861 gcccggcgcg gtgagcgtga tttcactgcc ctgccgcaga cccgtcacca gactttgcgc
     4921 attggcttcc gatccggcga aagccgcaaa actgttgacg atgcgggtct gggccggcgt
     4981 ttctcgaccg ttggcggtgg tagtagttgg tggattggtc tggtccgggg tctgcgcgcc
     5041 ggccgcgagg ttccccgcgg acagcacacc ggcaagcaat aatgctgaaa cagtcctcat
     5101 gacggctctc ctttcccgtt acccatagct ctttcggttt gaaggatgat ttcggtggcg
     5161 gtcgcgctgt cgaagaacca tccgtttctg gcgaccgttc gcatgcgcgt cgtgttcggg
     5221 gtaagcggtt cgagctggat ctcgatctcg cgcccggcgg cgttcgcctt gatcagctcg
     5281 ctgccttcgg ctttcgagct ggaggcgacc ttgatgccca tgcggttgag cgccgtcagg
     5341 gtcgcgccct tgaccttggg caggggggcg gtgaacgtgc ggtaggtgat gccgcccatc
     5401 gagtgggtga cacccgctga ggcgcccacc ccgaaagccg tgatcgagag cggctcgcag
     5461 ccagcgagcc ccgcggcccc ggcgaccagc aacagcgcaa tccggctttt catgccgccg
     5521 ctcattgccg gtcgccgggg gacaacgcga aagtgatgcg ccgtaccacc tcggtctccg
     5581 tttccgccgc gttatccgcc tcgcccgcgt cgcgcgcgat ctggcgcagc gcggcgagga
     5641 tttcgctcaa gtcccaggtg tccacgacat cgcgtgtcgg tccggccatg atgtcctccc
     5701 ttgtggttag cggtcagccc gcggcgacga tgccttgctc ggtcacgcgc actttctggc
     5761 cgacgctcag tgggggctgg gtatgctcat ggaacgtgcg aatgctgccg tcgttcatgc
     5821 gcactcggat ctcatagctg gctgacttct tcgcgttctt ctcgatttcg tggccggcgt
     5881 aggcgcccgc acccgcgccg acgaccgttg ccgccgtgcg ccctctgccg cgcccgacct
     5941 ggttgccgag gatgccgccg accacggcgc cgccgatggc gccgagcccg gtgccttcgc
     6001 ccggcacttc gaccacgctg atcgaatcaa ccacgccgca atcgttgcag gccgcggagg
     6061 cggatgccac cgcgcgcggg cggctcgcgg cgggcttcgt cgccgttggc gcggccttcg
     6121 tcgccgctgg cgcgtttgcc ggctgctcgc tcttcgactg cacgctcggc agccagccca
     6181 tcatggtggc gatgcctgcc acgctgaaca cgatcaccgc gatcgcagcg atcagcatta
     6241 cggggtagag gatgccggag cgtttttccc cggtatgttc cgttcccgtg gtattgacgg
     6301 tatgttccat tgcttgctcc ttttcctggc ccgaatgcgc cgccgggtgg tatccgccac
     6361 ccggcggcgt cgcgttcagc tcttgatggc caggccgttc ttcacctcga ttacgccctt
     6421 cacgccggcg gcaatttcag ccgcgcgccg cacctggttc tcgttgtcca cgaacccgga
     6481 cagcagcacg gtacccttgt aggtttgtac gccgaccgcc accgcgctca cgaggggatc
     6541 cttgagcagc gccgccttga tcttggcggt gatcacgctg tcctcgacgt actgaccggt
     6601 gagcatgcgg ccatggatcg cttgcgcctt cacgaattcg tcggcatcga gccgcccgtc
     6661 gcggttgtca tcggcttcct tgaacgcctg atcgaacccg cggatcttcc gggtttcctc
     6721 cagggtcaag taaccgtcgc cgttggcgtc gagcttcttg aattcccggt tgaactgtcc
     6781 atccgcttcg gaagcaccgg ctgcggccgc ggcgaggatc cagctcgcga ccagcgcgga
     6841 cactaccgcg cgatatccca cctgtttgct gttcatgtct cgctcctttc ttcagacaat
     6901 aagaaaaaaa cgaacacgag ctcgtgctca cgacagcctg ctgcaactgc cgtgccatgt
     6961 catcttttca cgcatattca atgagttaag tcgactgttc ggatgccggt tgtcgccgat
     7021 cgacaacaaa cgaattcaca tccgacttac gggtgtgatt cggcggggat ttttctgtcg
     7081 cgcgacggcg acaacagcga caaaacgcgc ccagtgggac gttcagcgac aacgaatgag
     7141 gaaaggcatc gcttcggaag cgcccgaggc gcgttttgat cggaaacctg ctcagggtcg
     7201 ctatcgaggg cgcgcgttga gcggcgcgtc ttagcgtccg ctcgaatgcg cgcaggtaaa
     7261 acggccgcgt agcggtccgt tttagcggga tttgaagagc ttgggatcga gctgatgccg
     7321 tccgagcagc ttgtagaact cggtgcggtt gcgcttggcg agccgcgccg cctgcgtcac
     7381 gttgccttcc gtgatcttga gcagctgcgt caggtattca cgctcgaagc ggcgccgtgc
     7441 ctcctcgaac gaggcgaact cggcctgctg gttctggatc gcctgctgcg ccagcgacac
     7501 cgggatgatg ggcgtggtgg caagggccac cgactgctcg accacgttgt agagctggcg
     7561 cacgttgccg ggccaggacg ccgccaccat catttccagc gcctccggcg cgaaaccgtt
     7621 gaccgccttc ttgtatttct ccgcgaggga cgacaggaaa tgattggcga gcagcgggat
     7681 gtcctcgcgc cgctcggtga gcggcggcag cgcgagcgcg accacgttca gccggtaata
     7741 gaggtcctcg cggaaattgc tcgccgccat ctccgcctcg aggttgcggt gggtcgcgga
     7801 aatcacgcgc acatccactt cgtgcgcttc ggtcgagccg accgccctca cttttttctc
     7861 ctgcagcacc cgcaagagct tcacctgcag cgagaggggc atgtcgccga tttcgtcgag
     7921 gaacagcgtg ccgcggttcg cggtctggaa cagccccttg ttgtcgcgca ctgcgccggt
     7981 gaacgcgccc ttcacgtggc cgaagagctc ggactcgagc aactgctcgg gaatcgcgcc
     8041 gcaattgatc gcgacgaagg ggtgatcgcg ccgcgggctc gcctggtgga tggcgcgcgc
     8101 gatcagctcc ttgcccgtgc ctgagtctcc ctggatgaac acgctcgcgt cgctttcggc
     8161 caccatccgc gccttggcga gcacgtcctc catcgcgggg ttgcgcgtaa tgataccccg
     8221 gcgccactcc tcgctggcgt cgccggccgc ttgggcgccg cccgccatgg cgagcgcccg
     8281 ctcgacctcg gcgagcagcg ccttcggatc gaacggcttg gtgagataac cgaatacgcc
     8341 gcgctggacc gcggccaccg cgtcgggaat ggtaccgtgg gcggtgagaa tgatgacggg
     8401 cagcgtgggg tgcccggcct ggatcgcctc gaacagcgcc atgccgttca tgccccccat
     8461 ccgcaggtcg gtcaccacca gttgcggacg ggagacgctc acctgggcga gcgcgcgctc
     8521 tccgctctcc gccgtcgata cggcatagcc cgcggcggtc aaccggatct gcatcagccg
     8581 caacaggtcc ggatcatcgt caacgaccag aatgttgatg cgcgcggcgt tcatcgcttt
     8641 ctctcgatca tgctgcgctc gagcgacttg agcgcgtcga gcttttgctg caggccctgc
     8701 gcgttggcgg cgagccgcct gcgctcctgc aggtggacag caagcaggta ggcgagcccg
     8761 cgcagctgac catcctggtt cctggaaacc ggctcgatca gctccagcgc gcgcccctcg
     8821 tcgctgaagg cggtgttggg aagcgagagt accatggcaa gccgcacacg gctgaaatcc
     8881 gacggcgagc cggtatacgc ctgcagcgcg gtttcgtgct cccgggcgag ctcggcgccg
     8941 gagagtttcc tgacgtgctg gtaatagagc agcaggcttt cagcgttcga ggcgggctga
     9001 gggctgccgt cctgtaccca gggcgccggc tcctgggttt cgacgccgcg cagcgcggcg
     9061 cagccggcga gcagggccgc gcaagctgga aaaacgagaa gccatcgcca cgtcatatgg
     9121 ccgcctctcc tcgcttgagg ggcacccgca cccgcaaatg cgcgccctgc cggttgtcca
     9181 cgacttccac gctgccgccg tgggcgaata cgtactcctt cacgacggag agcccgatcc
     9241 cggttccccg taccgggccc gcaccggcat ggcgcccctg gtagaaagga tcgaagatgc
     9301 gcgtgcgtt
//
LOCUS       DBNGWZ010000295         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_785651, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000295 DBNGWZ010000000
VERSION     DBNGWZ010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_785651"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="AC16CX_08630"
     CDS             <1..1138
                     /locus_tag="AC16CX_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012613.1"
                     /translation="GAESVTSLQDLLVSRGVTVAQVRPVSPSLEDVFVTRLAETAPAA
                     PPAPVAMSAGTGMEVAVQAQDLTMRFGNFTAVDGVSFSVRRGEIFGFLGPNGSGKTTA
                     IRILCGLLAPTAGRATVAGYDVGREPLAVKTRIGYMSQRFSLYEDMTVGENLAFFAGA
                     YGVPAAELPARRDRVLTLAGLNGAGARLTRSLSGGLKQRLALACAVLHEPHVLFLDEP
                     TAGVDPLSRRRFWELIYHLSDRGVTVFVTTHYMDEAEHCHTLGLLYSGRLIALGSPEA
                     LRTGMRAGEMLELECDQPIRALAIFQEAEGIRASFFGDRLHLLVDDTEAARPQIIERL
                     QREGHRIHRVERVPLSIEDVFIAFIEMEQTRREAAGANKANRVH"
     gene            1173..1793
                     /locus_tag="AC16CX_08635"
     CDS             1173..1793
                     /locus_tag="AC16CX_08635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3012614.1"
                     /translation="MPSFPKLTQTLIRQWDRHAVRYDRMSRPMERMLGFGNNRARLFE
                     GVTGAILELGAGTGRNLAHYPQGTWTVASDISQGMLAQARRVAQALGLSLPLVIADAE
                     DLPFRDASFDAVVATGVFCCVPDAVRGFQEARRVLRSGGRILLIEHVRPRGVVGWLFD
                     ALDPLVSRLMGPHINRATLESLKAAGFVIIREENLHSDWVKLIVAQ"
BASE COUNT          272 a          572 c          638 g          311 t
ORIGIN      
        1 gggggccgag tcggtcacct cgttgcagga cctgctcgtg tcgcgcggag tgacggttgc
       61 gcaggtccgt ccggtcagtc ccagcctgga ggatgtgttc gtcacgcgcc tcgcggagac
      121 cgcgccggcc gcgccgccgg cgccggtcgc gatgtcggcg ggaaccggca tggaggttgc
      181 ggtgcaggcg caggacctca cgatgcgctt cggtaacttc accgccgtgg acggggtgag
      241 cttttcggtg cggcgggggg aaatcttcgg gtttctcggc cccaatggtt cgggcaagac
      301 caccgccatc cgcatcctgt gcggcctgct cgcgcccacc gcgggccggg ccacggtggc
      361 gggatacgac gtcggccgag agcctctggc ggtgaagacg cgcatcggct acatgtcgca
      421 gcgcttctcg ctgtatgagg acatgacggt gggcgaaaac ctcgcgttct tcgccggcgc
      481 ctacggcgtg ccggccgcgg aactcccggc gcggcgcgac cgggtgctga ccctggcggg
      541 gctcaacggc gcgggagcgc ggctcacgcg ctccctgtcc ggcggcttga agcagcgcct
      601 ggccctggcc tgcgccgtgc tgcacgagcc gcacgtgctg tttttggacg agccgacggc
      661 cggcgtcgat ccgctgtcgc gtcggcgttt ttgggagttg atctaccact tatccgatcg
      721 gggcgtcacc gtgttcgtga ccacgcacta catggacgag gcggagcact gccacaccct
      781 cgggttgctc tactccgggc gtctgatcgc cctgggctca ccggaagcgt tgcgcactgg
      841 catgcgcgcc ggggagatgc tcgagctgga gtgcgatcag ccgatccggg cgcttgcgat
      901 ctttcaggag gcggaaggga tccgagcctc cttcttcggt gatcggctgc atcttttggt
      961 ggacgacacg gaggcggcga ggccacagat catcgagcgc ctacagcggg aaggtcatcg
     1021 gatccaccgg gtcgagcgcg tgcccctgag catcgaggat gtgttcatcg ccttcatcga
     1081 gatggagcag acccggcgcg aagccgcagg ggcaaacaag gcgaaccgcg tgcactagcg
     1141 cttgcccaag gacaaccaga aggaaggagc atatgccgag tttccccaag cttacccaga
     1201 cgctcatccg ccagtgggac cgtcacgccg tcaggtacga ccgcatgagc cggcccatgg
     1261 agcgcatgct gggtttcggg aacaaccgcg cccgcttgtt cgagggcgtg acgggcgcga
     1321 tcttggagtt gggcgcgggc accggccgca acctggcgca ttacccccaa ggcacctgga
     1381 cagtggcctc ggacatcagc caaggcatgc tggcccaggc ccgccgcgtg gcacaggcgc
     1441 tcggcttatc gctgcccttg gtgatcgcgg acgccgagga cctgccgttt cgtgacgcga
     1501 gcttcgatgc ggtggtggcg acaggcgtgt tctgctgcgt gcccgatgcg gtgcgcggat
     1561 tccaggaggc gcggcgcgtg ctcaggtcgg gtgggcgtat cctgctcatc gagcacgtgc
     1621 gtccgcgcgg cgttgtcggc tggctgttcg atgccttgga cccgctagtc agccggctga
     1681 tgggcccaca tatcaaccgc gccacgctgg aaagcctcaa ggccgcaggg tttgtcatca
     1741 tccgggaaga gaacctgcac tccgactggg tgaagctcat cgtggcgcag tag
//
LOCUS       DBNGWZ010000296         5074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2676590, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000296 DBNGWZ010000000
VERSION     DBNGWZ010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2676590"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16CX_08640"
     CDS             <1..208
                     /locus_tag="AC16CX_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012615.1"
                     /translation="DITTTLPYQPGATMQVGVGVLESAPSAAAPAAAMMGSNAREVGP
                     PAPVADVAPAPPPVSTSGGYQYRY"
     gene            complement(311..1528)
                     /gene="clsB"
                     /locus_tag="AC16CX_08645"
     CDS             complement(311..1528)
                     /gene="clsB"
                     /locus_tag="AC16CX_08645"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HIR3012616.1"
                     /translation="MGAHLRPRRVDGFSGAIVTEFVNGNRITLLKSGVEYFPALAAAF
                     DAAQREIHLETYIFEGDMTGRAIAGALMRAAGRGVATHVMVDGYGSKDLDRRLVGEMR
                     DAGVQLLVYRPNISPWTLRRARLRRLHRKIALIDARVTFVGGINVLDDADTPGGAPPR
                     FDYAVRVEGPLVARIYTVVKRLWALVSATQFRARLPHAVELSPPMEFRGGQRAAFVVR
                     DNLGHRRDIEQAYLAAIERAKNEILIANAYFFPGLSFRRALMDAAARGVRVALLLQGR
                     VEYILQHYASRALYGAFLDAGIEIHEYHKSFLHAKVAVIDRHWATVGSSNIDPFSLLL
                     AREANVVIEDDGFADELRGSLLAAIAGGAAQVRRERWGDQPWTNRVFTWGCYGLARFL
                     TGVFAYGRAQEFT"
     gene            complement(1474..2232)
                     /locus_tag="AC16CX_08650"
     CDS             complement(1474..2232)
                     /locus_tag="AC16CX_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3012617.1"
                     /translation="MRTLRVATYNIHKGLSVTPLLSRRLTIHELREKLRALDADIVFL
                     QEVHGKHERHARRYENWPTAPQYEFLADAVWSDFAYGRNSLYDAGHHGNAILSRFPIL
                     TWDNQDVSSHRFERRGLLHCEIRIPRWREPLHCVCVHLGLRGHERSRQLEKLRQRIER
                     LVPPEAPLIVAGDFNDWRYRAAQELAHPLNLYEVFELVKGRPARTFPAALPVMSLDRI
                     YVRGFKVPTAHVHHGRAWARISDHAALTASLVRL"
     gene            complement(2330..3907)
                     /locus_tag="AC16CX_08655"
     CDS             complement(2330..3907)
                     /locus_tag="AC16CX_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012618.1"
                     /translation="MTSDRKQGVSGASHNPVTSHESRVTHSSFGTALLLCCVLISGCA
                     IAPRQAAYADPNEECREVFQRVDQTVAEAGVQDGMAARVAGFPYLRVDRFLASYADEE
                     MGDAQFAEWMNRMIELGQRGHAIEIANLLADRAEALGHSLWHIGRGHAWVAPALSDCT
                     ARLAAADYANPARRAALKSTARVPDDYVSWHRVAGLYWLTRIPFAAGIRNWHREAREV
                     FSQPLDALPVAGRLEIYAPPPGRLAREGLSALLARAPANPLGIPEPRGADLEALFRAF
                     APEFVVDTAGESDRPGDLGWGEDGVPRVVSRRLVVYRRLSHTRHEGRALLQLNYSIWF
                     PERPKGAGWDILGGQLDGVLWRVTLAPDGEPWVFDSIHLCGCYHQFFPTARATPRAQP
                     DTLDETAFVPQTLPRIDPGARVMLRLEAGTHYLQRVVIHAGSTEPAVEYIFEDDDALR
                     SIPLPGGGRRSVFRPDGIVPGSERGERWLFWPMGVPEPGAMRQWGRHATAFVGRRHFD
                     DPDLLERYFTLESMSRE"
     gene            complement(3904..4464)
                     /locus_tag="AC16CX_08660"
     CDS             complement(3904..4464)
                     /locus_tag="AC16CX_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3012619.1"
                     /translation="MRPATLRFVCLLAGGLATAAPAAEVTVHATRNGDVLEVVAVAEF
                     EGDIALTWRVLTDYGRIAEYVPNLETSRIVSRDRNGAVVDQKGKARMLFFSYPINVRL
                     AVTEHPYERVESRAVAGNFREMRNSYTLETRQGRVLLRYAGRLVPDFTIPPLIGPWIL
                     RRNVEEAFRALVEEIERQQKPVTSDR"
     gene            complement(4646..>5074)
                     /gene="nadA"
                     /locus_tag="AC16CX_08665"
     CDS             complement(4646..>5074)
                     /gene="nadA"
                     /locus_tag="AC16CX_08665"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3012620.1"
                     /translation="HCSVHQMFQPQHILRWREKHPSGMVISHPECRFDVCKLSDYVGS
                     TDFIIRTIAASPPGTRWLVGTELNLVNRVAEQFKSQGKVVQFMATTVCMCSTMARIDP
                     QHFAWALENLVAGKVVNQIRVPKHEAELARIALDRMLAVS"
BASE COUNT          881 a         1711 c         1661 g          821 t
ORIGIN      
        1 cgacatcacc acgacgctgc cgtaccagcc gggcgcaacc atgcaggtcg gcgtcggcgt
       61 gctggaaagc gcgccatcgg cagcggctcc cgccgccgcc atgatggggt ccaacgcgcg
      121 cgaggtcggc ccgccggccc cggtcgccga tgtcgcgccg gcgccccctc cggtcagcac
      181 gagcggcggg tatcagtatc gttactagcg gtcagcgatc agctgtcagc catcagctta
      241 aaacagaagg cgcgctttca ggtgccgcgc ctcgtttggc cgaccgctga atgctgatag
      301 ctgacagctc tcacgtaaac tcctgcgccc ttccgtaggc gaacacgccg gtcaggaagc
      361 gcgcgagccc gtagcagccc caggtaaaga cgcggttcgt ccacggctgg tccccccagc
      421 gctcgcgccg tacctgcgcc gctcccccgg cgatggcagc aagcaggctc ccgcgcagct
      481 cgtcggcgaa gccgtcgtcc tcgattacga cgttggcctc gcgcgcgagc agcaggctga
      541 aggggtcgat gttggaagag cccactgtcg cccagtgccg atcgatcact gcgaccttgg
      601 cgtggaggaa gctcttgtgg tactcgtgga tctcgatgcc cgcgtcgaga aatgctccgt
      661 agagagcgcg cgaggcgtag tgctgcagga tgtactccac cctgccctgc agcagcagcg
      721 cgacgcgcac tccccgcgcg gccgcgtcca tcagcgcgcg ccggaagctg agccccggga
      781 agaaataggc gttggcgatc aggatttcgt tcttcgccct ctcgatcgcc gcaaggtagg
      841 cctgctcgat gtcgcggcgg tgtcccaggt tgtcgcgcac cacgaacgcc gcgcgctgcc
      901 caccgcggaa ttccatcggc ggcgagagct cgacggcatg gggcaggcgc gcgcggaatt
      961 gcgtggcgct caccagcgcc cacagccgct tcacgacggt atagatcctc gcgaccaggg
     1021 ggccttcgac gcgaacggcg taatcgaagc gcggcggcgc accccccggc gtgtccgcgt
     1081 cgtccaagac gttgatgccg ccgacgaagg tgacgcgcgc gtcgatcaac gcgatcttgc
     1141 ggtgcaggcg gcgcaggcgc gcgcgccgca gagtccaggg cgagatgttc gggcggtaga
     1201 cgagtagctg gacgccagca tcccgcatct cgccgaccag cctccggtcg aggtctttcg
     1261 atccatatcc gtccaccatg acgtgggttg ccacgccgcg ccccgccgcg cgcatgagcg
     1321 cgcccgcgat ggcgcgcccc gtcatatcgc cctcgaaaat atacgtttca aggtggatct
     1381 cgcgctgcgc ggcgtcgaat gccgccgcca gcgcggggaa atactccacc ccgctcttca
     1441 gcagggtgat gcggttgccg ttgacgaact cggtcacaat cgcaccagag aagccgtcaa
     1501 cgcggcgtgg tcggagatgc gcgcccatgc gcgcccgtgg tggacgtggg cggtgggcac
     1561 cttgaagccg cgcacgtaga tgcggtcgag cgacatgacc ggcagcgccg cgggaaaggt
     1621 gcgcgcgggc cggcccttca ccagctcgaa cacctcgtag aggttgagcg ggtgcgccag
     1681 ctcctgcgcc gcgcggtaac gccagtcgtt gaaatcgccc gcgacgatca gcggcgcctc
     1741 cggcggcacc aggcgctcga tgcgctggcg cagcttttcc agctggcggc tgcgctcgtg
     1801 gcccctcaag ccgaggtgca cgcacacgca atgcagcggc tcgcgccagc gcgggatgcg
     1861 gatctcgcaa tgcagcagcc cgcgccgctc gaagcggtgg ctggaaacat cctggttgtc
     1921 ccacgtgagg atcgggaagc gcgacaggat cgcgttgccg tgatggccgg cgtcgtagag
     1981 cgagttcctg ccataggcga agtcgctcca caccgcgtcg gcgaggaatt cgtactgcgg
     2041 cgcggtcggc cagttctcgt agcgccgggc gtggcgctcg tgcttgccgt gcacttcctg
     2101 caggaacacg atgtcggcgt cgagtgcgcg cagcttctcg cgcaactcgt gtatcgtgag
     2161 ccgccggctg agcagcggtg tcacggacag gcccttgtgg atgttgtagg tcgctacgcg
     2221 aagcgttcgc atcgattcaa aagcgtgatt ggtgaatggt gaatagtgaa tggacaagag
     2281 cccttgatgc aaaccgcatt gttgcctggt tcagtccact caccactcac tactcacgac
     2341 tcatgctttc caaggtgaag tagcgctcaa ggagatcggg atcgtcgaag tgccgccggc
     2401 cgacgaaggc ggtggcgtgc cgcccccact gccgcatcgc accgggttcg ggcacgccca
     2461 tcggccagaa cagccagcgc tcgccgcgct cgctgcccgg aacgatgcca tccggccgga
     2521 acacgctgcg ccgcccgcct cccggcaagg gaatcgatcg caatgcgtcg tcgtcctcga
     2581 aaatatactc gaccgccggc tccgtcgatc cggcgtgtat gaccacacgt tgcagataat
     2641 gagtacccgc ctcgagccgc agcatgacgc gcgcgccagg gtcgatgcgc ggcagggtct
     2701 gcggcacgaa agccgtttcg tccagcgtat cgggctgcgc tctcggcgtc gcccgcgcgg
     2761 tcgggaaaaa ctggtggtag cagccgcaga ggtggattga gtcgaacacc cacggctcgc
     2821 cgtcgggcgc gagcgtgacc cgccacagca cgccgtcgag ctgtcccccc aggatgtccc
     2881 agccggcccc tttggggcgc tccgggaacc agatcgaata gttgagctgc aacagcgcgc
     2941 gcccctcgtg gcgggtgtgc gacagccggc ggtagaccac cagtcggcgc gacacgacac
     3001 ggggcacgcc gtcttcgccc caccccagat cgccgggcct gtccgattcg cccgcggtgt
     3061 ccacgacgaa ctccggggcg aacgcgcgga acagcgcttc gaggtccgcg ccgcgcggct
     3121 cgggtatgcc gagcggattc gctggtgcac gtgcgagcaa ggcggaaagt ccctcgcgcg
     3181 cgagccgccc gggcggcggc gcatagattt ccagccggcc cgcaacaggc aacgcatcga
     3241 gcggctgcga gaacacctcg cgcgcctccc tgtgccagtt gcgtattccg gccgcaaacg
     3301 ggatgcgagt gagccagtac agtcccgcca ccctgtgcca gctcacgtag tcatccggga
     3361 cgcgggccgt gcttttcagc gcggcacgac gcgccggatt cgcgtaatcg gccgcggcca
     3421 gccgtgccgt gcaatccgac agcgcgggcg cgacccacgc atgaccccgg ccgatgtgcc
     3481 acagggagtg tccgagcgct tcagcccgat ccgcgagcag gttggcgatt tcgatcgcgt
     3541 gcccccgctg gccgagctcg atcatgcggt tcatccattc ggcgaattgc gcgtcgccca
     3601 tctcctcatc ggcgtagctc gccagaaagc ggtctacgcg caagtacgga aaacctgcga
     3661 cacgcgccgc catgccgtcc tggacgcccg cctcggctac cgtctggtcc acgcgctgga
     3721 atacctcccg gcattcctcg ttcggatcgg cgtaagcagc ctgacgcggc gcaattgcac
     3781 agccggaaat caggacgcag cacaggagca acgccgtccc gaacgaggaa tgcgtgactc
     3841 gtgactcgtg actcgtgact ggattgtggc tcgcgccgct cacgccttgt ttccggtcac
     3901 tggtcaccgg tcactggtca ccggcttctg ctggcgctcg atctcttcga ccagggcgcg
     3961 gaacgcttcc tcgacgttgc gcctgagaat ccaggggccg atgagcggcg gaatggtgaa
     4021 atccggcacg aggcggccgg cgtaacgcag cagcacgcgc ccctgccgcg tttccagcgt
     4081 gtagctgttc cgcatctcgc ggaagttgcc ggcgactgcg cgcgactcga cccgctcgta
     4141 gggatgttcg gtgacggcaa gccgcacgtt gatgggatag ctgaagaaca gcatccgcgc
     4201 ctttcctttc tggtccacca cggcgccgtt gcggtcgcga gaaacgatgc ggctcgtctc
     4261 cagattcggc acgtactccg cgatgcggcc gtagtcggtg agcactcgcc aggtaagtgc
     4321 gatgtcgccc tcgaactcgg ccaccgccac cacttccaga acgtcaccgt tgcgcgtggc
     4381 gtgaaccgtc acttccgcgg ccggcgccgc cgtcgcgagc cctcccgcca ggaggcaaac
     4441 aaaccgcagt gtggccgggc gcatggtgca tagcgtaaag gattttggcg accctggtcc
     4501 gccaaaatcc tcatagaacc caggaaatct tagccggtcg cgggaaagaa actggggatg
     4561 aggggactct aatgcctctg tccggagcaa aagtaaagga cgacttggaa tcaggaattt
     4621 gtcggaacag aatccgacaa attcctcagg agaccgcgag catgcggtca agggcgatgc
     4681 gcgccagctc cgcctcgtgc ttcggcaccc tgatctggtt gaccactttg ccggcaacga
     4741 gattttccag cgcccaggcg aaatgctgcg gatcgatgcg cgccatggtc gagcacatgc
     4801 acaccgtcgt cgccatgaac tgaaccacct tgccctgcga cttgaactgc tcggcgacgc
     4861 ggttcacgag gttcagctcg gtgcccacca gccagcgcgt gcccggcggg ctcgccgcga
     4921 tggtccggat gatgaagtcg gtggagccga cgtagtcgga gagcttgcac acgtcgaacc
     4981 tgcactcggg atgcgagatc accatgcccg agggatgctt ctcgcgccag cgcaggatgt
     5041 gctgcggctg gaacatctgg tgcaccgagc aatg
//
LOCUS       DBNGWZ010000297         4872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_537746, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000297 DBNGWZ010000000
VERSION     DBNGWZ010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_537746"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1880
                     /gene="dnaG"
                     /locus_tag="AC16CX_08670"
     CDS             120..1880
                     /gene="dnaG"
                     /locus_tag="AC16CX_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3012621.1"
                     /translation="MIPQSFIQDLLHRVDIVDVVDRHVKLKRAGANYAACCPFHNEKT
                     PSFKVSPSKQFYHCFGCGAHGTAVGFLMEYGGMGFVEAVKDLAQSVGMKVPEVRSETA
                     QRRAQQGESLYEVLLRAAQHYRNRLKDAPHAIAYLKNRGLSGEIAKRFGIGYAPDEWQ
                     GLAAAFTDYDGKALAEAGLTKTNEEGRRYDVFRNRIMFPIVDVRGNVIGFGGRVLGEG
                     EPKYLNSPETPVFEKGRELYGLYQARRAIRDAGRVLVVEGYMDVVALAQAGVEYAVAT
                     LGTATTPLHVQKLLRQADEIVFCFDGDEAGRRAAWRALENSLAQLQDGKQVKFLFLPE
                     GEDPDTYVRKHGKTALEALLASGVPLSKFLMDELTRRVDLSTAEGRARCVHQAKPLLK
                     QMQANALRMQLVRELAEKCRLSPEEIAQLCELAPAAARPRAPEPGYPRTPPPPLVEQL
                     LQLLVSKPGCVEKLSAEHRALLDSPEFAPVAELVDTLKESGATTSAMLEEVTRESQHA
                     RLYQDVAARTLTDAMDDETARKILADVLLKLERQRMESEYRRLNTGKESSEVERQRFQ
                     EVSRRLDELKRATTGSRTEI"
     gene            2069..4315
                     /gene="rpoD"
                     /locus_tag="AC16CX_08675"
     CDS             2069..4315
                     /gene="rpoD"
                     /locus_tag="AC16CX_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR3012622.1"
                     /translation="MAKSTKSKSRAKAVARRKAHAKARHGAKRHAAKSKAKQLKSRAK
                     VRHAAALKAMLARKDLRAPKLLPKGEVLPKPKTGKVPQKMDKATLKKALLQERIARAQ
                     AKAEGKRSTRGLTAKERALVKEFERKELSPADAEARRTRLKNLIILGKERSYLTYAEI
                     NDHLPDDMLDAELIENVISMINDMGIQVYDEAPDAETLLMSEVTPPVADEEAVEEAEA
                     ALSTVDSEFGRTTDPVRMYMREMGSVELLTREGEIEIAKRIEDGLRHMIQAISACPTT
                     IAEILALAEKIEKDEIRADEVVDGLVDPNARAEPIKEVSEDDEDLEEELEAAEEEDEE
                     GGAVQSADQLKLKADALKRFAVIRSLYARMTRALARNGPRSRAHLQSRDQISEELMKI
                     RFSAKQIEALCDSVRKLVEEVRTYERNIMEICVVKAKMPRPHFIKEFPGKEGNLRWIK
                     SEIDTGHPWSEALERFEPAIVEQQQKLLNLQARVGIPIKDLKEINRQMSTGEAKARRA
                     KREMTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATW
                     WIRQAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPDPALLAEKMEMPE
                     EKIRKILKISKEPISMETPIGDDDDSHLGDFIEDQSTVAPNEAAVYASLRGVTKDVLD
                     TLTPREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERL
                     RSFLDQDS"
     gene            4436..4509
                     /locus_tag="AC16CX_08680"
     tRNA            4436..4509
                     /locus_tag="AC16CX_08680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4470..4472,aa:Ile,seq:cat)
     gene            4613..>4872
                     /locus_tag="AC16CX_08685"
     CDS             4613..>4872
                     /locus_tag="AC16CX_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR3012623.1"
                     /translation="MATIQARRTQGGTRYRVQVRLRGHPPISASFERRTEARKWVQQT
                     EAAIREGRYFPTHEARRRTLADLVDRQLEAVKAKRPHDYDRQR"
BASE COUNT         1040 a         1499 c         1596 g          737 t
ORIGIN      
        1 tcggtgcttc gcgcgttacc gcattttcgt tattccgcca cgggcgggca cggctatact
       61 ctgggcatgc agggaaaccg gccacccgcc agacttcgtt cgccacgccg tgcccgcgaa
      121 tgattcccca gtcgttcatt caggatctgc ttcaccgtgt cgatatcgtc gacgtggtcg
      181 accgccatgt caaactgaag cgcgcaggcg cgaactacgc ggcgtgctgc cccttccaca
      241 acgaaaagac cccgtcgttt aaggtcagtc cctccaagca gttctatcac tgcttcggct
      301 gcggcgcgca cggcacggcg gtcggcttcc tgatggagta cggcggcatg ggcttcgtcg
      361 aggcggtgaa ggatctcgcc caaagcgtcg gaatgaaagt gcccgaagtg cgctccgaaa
      421 cggcgcagcg ccgggcccag cagggtgaaa gtctttacga agtgctgttg cgcgcggcgc
      481 agcactaccg caaccggctc aaggatgcgc cgcatgccat tgcctacctc aagaaccgcg
      541 ggctttccgg cgagatcgcc aagcgcttcg gcatcggcta cgccccggac gaatggcagg
      601 gcctggcggc ggcgttcacg gactacgacg gcaaggccct tgcggaggcg gggctcacca
      661 agaccaacga ggaaggccgg cgctacgacg tgttccgcaa ccgcatcatg ttcccgatcg
      721 tggacgtgcg cggcaacgtg atcggctttg gcggtcgggt cctgggcgaa ggagagccga
      781 aatacctcaa ttccccggaa acgccggtgt tcgagaaagg gcgcgagctc tatggcctct
      841 atcaggcgcg gcgcgcgatc cgcgacgccg gccgggtgct ggtggtcgag ggttacatgg
      901 acgtggtcgc gctcgcgcag gccggcgtcg aatacgcagt ggcgaccctg ggcaccgcga
      961 cgacgccgct gcacgtgcag aagctcctgc gccaggccga cgagatcgtg ttctgcttcg
     1021 acggcgacga agcgggccgg cgcgccgcct ggcgcgcgct cgagaacagt ctcgcgcagc
     1081 ttcaggacgg caagcaggtg aagttcctgt tcctgccgga gggcgaggac ccggacacct
     1141 acgtgcgcaa gcacggcaaa acagcattgg aggccctact cgcaagcggc gtgccgttgt
     1201 caaagtttct gatggacgag ctgacgcgcc gggtggatct gtccacggct gaaggccgcg
     1261 cccggtgcgt ccatcaagcg aaaccgctgc tcaagcagat gcaggcaaac gcgctcagga
     1321 tgcaactggt tcgggaactg gcggagaaat gccgcctgtc gcccgaggag atcgcgcaac
     1381 tttgcgagtt ggcgcccgca gccgccaggc cccgtgcgcc ggagccggga tacccgcgca
     1441 cgccgccacc gccccttgtg gagcagttgc tgcagttgct ggtttccaaa ccgggttgcg
     1501 tggaaaaact ttcggcggaa catcgggcgc tgctcgactc gccggaattt gctccggttg
     1561 cggaactggt cgatacgctc aaggaaagcg gtgccacgac gtccgcaatg ttggaggagg
     1621 tgacgcgcga atcgcagcac gccaggcttt accaagacgt cgcggcgagg accttgactg
     1681 acgctatgga cgacgaaacg gcgagaaaga ttcttgcgga cgtgctcctc aagcttgagc
     1741 ggcagcgcat ggagagcgaa tacaggcgct tgaacaccgg caaggagagc agcgaggtcg
     1801 agcgccagcg tttccaagag gtatcccgac gcctggacga actgaaaagg gccacgacgg
     1861 gctcccgcac cgagatttga atgaaatcgc gcattgaagt gttcaccctg cgaccccaca
     1921 tacatacttc attccggcca cgccgagctg gcgcaaaaat tgctttggcg acaatcagtt
     1981 gggcggtaac tttgtcgtgt ggccggggtc gaacaagagt ataattcgcg gtttttttga
     2041 cgtagcaagt ccacgcggga acggcgagat ggcgaaatcc acgaaatcga agtccagggc
     2101 gaaagccgtg gcgcgcagga aggcccatgc caaggcgcgc catggcgcaa aacggcatgc
     2161 ggccaaatcg aaggcgaagc agctcaagtc gcgggccaag gtccggcatg ccgccgccct
     2221 gaaggcgatg ctggcgcgca aggatctgcg cgcccccaag ctcctgccca agggggaggt
     2281 actgcccaag cccaagaccg gcaaggtccc gcagaagatg gacaaggcga cgctgaagaa
     2341 ggcgttgctg caggagcgca ttgcccgggc gcaggcaaaa gcggaaggca agcgctctac
     2401 gcgcggcttg accgccaagg aacgggcgct ggtcaaggag ttcgagcgca aggaattgtc
     2461 gcccgctgac gccgaggcgc gccgcacgcg gctgaagaac ctcatcatcc tcggcaagga
     2521 gcggagctac ctcacctacg ccgagatcaa cgaccacctg ccggatgaca tgctggacgc
     2581 cgagctgatc gaaaacgtca tcagcatgat caacgacatg ggcatccagg tctatgacga
     2641 ggcgccggat gccgaaacgc tgctgatgtc ggaggtgacc ccgccggtcg ccgacgagga
     2701 ggcagtcgag gaagcggagg ccgcgctttc tacggtggac tccgagttcg gccgcaccac
     2761 cgacccggtg cgcatgtata tgcgcgagat ggggtcggtg gagctcctca cgcgggaagg
     2821 cgagatcgag attgccaagc gcatcgagga cgggctgcgc cacatgatcc aggcgatctc
     2881 ggcctgtccc accaccatcg ccgagatcct cgcgctcgcc gagaaaatcg agaaggacga
     2941 gatccgcgcg gacgaggtgg tggacggttt ggtggatccc aacgccaggg cagaaccgat
     3001 caaggaagtg tccgaggacg acgaggacct cgaggaagag ctcgaggcgg ccgaggagga
     3061 agacgaggaa ggcggagccg tgcagagcgc ggaccagttg aagctcaaag ccgacgcgct
     3121 caagcgcttc gccgtgatcc gctcgctcta cgcccggatg acgcgcgcgc tcgcccgcaa
     3181 cgggccgagg agccgcgccc acctccagtc gcgcgaccag atctccgagg agctcatgaa
     3241 gatccgcttc tccgcgaagc agatcgaagc gctgtgcgat tccgtccgca agctggtgga
     3301 ggaagtgcgc acctacgagc gcaacatcat ggaaatctgc gtggtcaagg cgaagatgcc
     3361 acgcccgcat ttcatcaagg agttccccgg caaggagggg aacctgcgct ggatcaagag
     3421 cgagatcgac acgggccacc cctggagcga ggcgctcgag cgcttcgagc cggcgatcgt
     3481 cgagcagcag cagaagctct tgaatctcca ggcgcgggtc ggcatcccga tcaaggacct
     3541 gaaggagatc aaccgccaga tgtccaccgg cgaagcgaag gcgcgccggg cgaaacgcga
     3601 gatgaccgag gccaacctgc ggctggtgat ctcgatcgcc aagaagtaca ccaaccgcgg
     3661 gctgcagttc ctcgacttga tccaggaggg caacatcggc ctgatgaagg cggtggacaa
     3721 gttcgagtac cgccgcggct acaagttctc gacctacgcc acgtggtgga tccggcaggc
     3781 gatcacacgc tcgatcgcgg accaggccag gacgatccgg atcccggtgc acatgatcga
     3841 gaccatcaac aagatgaacc gcatctcgcg ccagatcctg caggagaccg gccaggagcc
     3901 cgatcccgcc ctgctcgccg agaagatgga gatgccggag gagaagatcc gcaagatcct
     3961 caagatctcc aaggagccga tctcgatgga aacgccgatc ggcgacgacg acgattccca
     4021 cctcggggat ttcatcgagg accagtccac cgtcgcgccg aacgaggcgg cggtctacgc
     4081 gagcctgcgc ggcgtcacca aggacgtgct ggataccctc actccgcgcg aggcgaaggt
     4141 gctcaggatg cgcttcggca tcgagatgaa caccgaccac acgctggagg aggtcggcaa
     4201 gcagttcgac gtcacccgcg agcgcatacg ccagatcgag gcgaaggcgc tgcggaaact
     4261 gcgccatccc tcgcgctccg agcggctgcg cagcttcctc gaccaggaca gctaagagca
     4321 aggcgaaagg cgaaagtcgg aaggcgaaag tcctttcgcc gcccgccgcc gcgcgcaaaa
     4381 aggcgggttt tacttttgcc tttcgacttc cgactttctc cttataattc ctggggggtc
     4441 tgtagctcag ccggtcagag caggggactc ataatccctt ggtcgttggt tcgaatccaa
     4501 ccagacccac tctgtaaatc aataagttac gtagttaaag cagacccgga tttgcatttg
     4561 cggcacactt ttggcacact tcacggtaga tttccagcgt ggagggccgc aaatggcaac
     4621 gattcaggca aggagaaccc aaggcggaac gcgctaccgg gtgcaggtcc ggctgcgggg
     4681 gcatccgccg atatcagcga gcttcgagcg gcgcaccgaa gcgcgtaagt gggtgcagca
     4741 gaccgaggcc gcgatccgcg agggccgcta cttcccgact catgaggcca ggcgccgaac
     4801 cctggccgac ctggtggacc gccagctaga ggcggtcaag gcgaagcgcc cgcacgacta
     4861 cgaccggcaa cg
//
LOCUS       DBNGWZ010000298         6648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_660857, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000298 DBNGWZ010000000
VERSION     DBNGWZ010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_660857"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16CX_08690"
     CDS             <1..234
                     /locus_tag="AC16CX_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012624.1"
                     /translation="ARFPREMTGRVVTALNMVTFSLAFAVQYGVGAIIDLWPVTDGRY
                     APEGYRAAFALCWVLQVAAVAWLAYSERGALRR"
     gene            460..1035
                     /locus_tag="AC16CX_08695"
     CDS             460..1035
                     /locus_tag="AC16CX_08695"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3012625.1"
                     /translation="MHVATHLLIGWTLAEHTTKSPRDRALITWASVVPDLDGLGLLVD
                     ALVQQFGGTMQWYDRFHHYLLHGLPGALLCTGLFVCFASERIRAACLILVSYHLHLLG
                     DLLGSRGGGETAIWPVHYLAPLSEELTFAWSGQWPLTGWQNTTLTVVLMIYVLGLAVR
                     RGYSLVGLFSTRADIVFVETLRRRWRSLRRV"
     gene            1051..2025
                     /locus_tag="AC16CX_08700"
     CDS             1051..2025
                     /locus_tag="AC16CX_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012626.1"
                     /translation="MTKNLLIVTAFAMVASAAGFARAQTENYPAKPLRLISPFPPGGS
                     VDLVARMIAAKLPDFLGQQVVVDNRSGASGNIGTELVARSAPDGYTLLANTIPFVANA
                     FLYSKLPYDPLNDFAPIMQVSSSPNVLVVHPSLPVRSVKELLALARSKPGGLNYGSAG
                     PATNPHIAGELFNYLGKVNIVVVHFKGGGPALVAAISGEVGIAFTNFAETSEFVKAGR
                     LRALGVTSAKRNPAMPDLPTIAEAGLPGYEFTTWHGIWAPKGTPRAIVALLNDRLKKT
                     MAAPDQKKRFEDRGLDVIASSPEEFSAHLQSEYKKWGRVIKERGMKAD"
     gene            complement(2111..2794)
                     /gene="nthA"
                     /locus_tag="AC16CX_08705"
     CDS             complement(2111..2794)
                     /gene="nthA"
                     /locus_tag="AC16CX_08705"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HIR3012627.1"
                     /translation="MSDSSFDYYAHRKHGHDHGRDHHAPSAAEDEGPPSEHEIMSRAM
                     QELLEAKGVITAEEVRRRMELFEEEFPYRGSRVIARAWIDPAFKKRLLKDGKAACAEM
                     GVDLEATHLIAVENTPNVHNVIVCTLCSCYPRELLGMPPTWYKSSNYRSRVVFEPRAV
                     LKEFGTVLPDHVTVRVHDSNADMRYVVVPMRPEGTEGWSEEQLAKILTRDTLVGVTVP
                     HVPAKSRRF"
     gene            complement(2801..3184)
                     /locus_tag="AC16CX_08710"
     CDS             complement(2801..3184)
                     /locus_tag="AC16CX_08710"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HIR3012628.1"
                     /translation="MTRPGRRRGAKPLPSTYTPAPGHLFEVGDRVVVKRIFPPGHRRT
                     PYYIRGKQGVIERICGTFRNPEELAYGFEGKPKKVLYRVRFPQKHVWPDYRGPKRDVV
                     EMEIFEHWLEPASGRPEPADPDESG"
     gene            complement(3181..3540)
                     /locus_tag="AC16CX_08715"
     CDS             complement(3181..3540)
                     /locus_tag="AC16CX_08715"
                     /inference="COORDINATES: protein motif:HMM:NF045268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012629.1"
                     /translation="MSDRPLNKGPHDMGGEAAGPIDTTDHGMKFWERQANALRNTLTR
                     KKIVLVDELRRAAEDLGEQYYKLDYFQRTTSALRNLLLEKGLITEAELKAKMAEVLAR
                     FDVPGKTRSPGDKGAGR"
     gene            3739..4653
                     /locus_tag="AC16CX_08720"
     CDS             3739..4653
                     /locus_tag="AC16CX_08720"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3012630.1"
                     /translation="MGIKLRGVMQSPVTPLKEDFSPDLPTFERLLDFHVRTSGTAISW
                     PHHKGESLNLTIEERKRFAEVAVKTVAGRVPVSIHVSALSVEDSLDLARHAEKIEADA
                     IIAITPYFWNPSVEAIYEHFVRIGTAVDLPLIAYNSPSYLSGIEFTGELVTRLLERLP
                     NFIAMKEASFNSEKFLEIARAALALRPDFALVTGVEYLLPSVPLGGVGSYSSAGAICP
                     NLCTELFESCVAADWPRARECQYKLSQLWLLFRDQYPSSLKGGMVMMGRPVGPTRQPL
                     PTATPERRAYIRGQLEELGILQTEPHGW"
     gene            4756..5670
                     /locus_tag="AC16CX_08725"
     CDS             4756..5670
                     /locus_tag="AC16CX_08725"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3012631.1"
                     /translation="MANFELGLVHTPVTPFTHERRIDYDLYEKLIGFHLRNGADSLAL
                     PMHAGESVSLSDEERKTLLEFAIGKVKGRVPVIAHVSQSGTGMAVALARHAEQAGAAA
                     IVATVPYYWTPPPAMLVEHFTQIGSAARLPFFVYNSPEEMGGVRVTTEIALKLIERLD
                     NFAGVVDSSLDWQFMIEVISVARRKRASFQLLSGTEYMISAGAIGSTGVFAPLAGIAP
                     KVVRGLYELCREERYGDGRRNQEAIAALRQAVKRDGVAGLKGAMRAMGRDCGGPRPPL
                     RPLGEVEYGALAERLGALAFLSAESKGW"
     gene            5788..6540
                     /locus_tag="AC16CX_08730"
     CDS             5788..6540
                     /locus_tag="AC16CX_08730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012632.1"
                     /translation="MTKERRAAFVTGASQGIGAAIAVALARDGFDVAVSSTRPEKLSG
                     VLTDIGAAGGRSAPVALDVRSQASIEQAMAHVVSAFGHLDVLVNNAGVPLKKPALEVS
                     GAEWDAVIGTNLTGTFFMSQQMGRHLVKEGRPGCIISIASTHGLVGLAGRSIYGIAKA
                     GMIHMTRMLAIEWAEHGISVNAIAPGRVDTPSRAGSLAEPGYRDAALARIPLKRFATA
                     EEIAAAVCYLVSPAATYITGQTLVLDGGLTAA"
BASE COUNT         1132 a         2146 c         2178 g         1192 t
ORIGIN      
        1 gcgcgctttc cgagggaaat gacgggccgc gtggtgaccg cgctcaacat ggtcaccttt
       61 tcgctcgcgt tcgcggttca gtacggcgtc ggcgcgatca tcgacctgtg gccggtgacc
      121 gacggccgct atgcgcccga gggatatcgc gccgcgttcg cgttgtgctg ggtgctgcag
      181 gtcgccgcgg tggcgtggct cgcctactcg gaacgcggcg ctctgcgccg ctaaggcaga
      241 aggcagaagg cagaaggcag aaggcagaag gcagaaggca gaaggcagaa ggcagaaggc
      301 agaaggcaga aggcagaagg cagaaggcaa caagacgctg gtcaacgccg ttttctacac
      361 ttcacccttc tgccttcatc cttcatcctt aggttatcct tcatccttct gccttcatcc
      421 ttgattcaat gtaaccccct catccacaat ctgacagcca tgcacgtcgc gacccatctg
      481 ctcatcggct ggacgctcgc ggagcacacc acgaaatcgc cgcgagaccg cgccctgatc
      541 acgtgggcga gcgtggtgcc ggacctcgac ggcctggggc tgctcgtgga cgcgctggtg
      601 cagcagtttg gcgggacgat gcagtggtac gaccggttcc accattatct gctgcatgga
      661 ctgcccggcg cgctgctgtg caccgggctt tttgtctgtt tcgccagcga gcggatacgc
      721 gcggcgtgcc tcattctggt ctcgtatcat ctgcatctgc tgggggatct gctgggttca
      781 cggggaggcg gggagacggc catctggccg gtccattacc tggcgccgct ctccgaggag
      841 ctgacgttcg cctggagcgg gcagtggccg ctcacgggct ggcagaatac gaccctcacc
      901 gtcgtgctga tgatctatgt gctcgggctg gcggtccggc gcgggtattc gctcgtaggc
      961 cttttcagca cgcgggccga catcgtgttc gttgaaacgc tgcgccggcg ctggcgttct
     1021 ttgcggcgcg tctgagaagg aggacaagtc ttgacaaaga acctgttgat cgtcacggct
     1081 tttgcgatgg tggcatccgc cgccggattc gcgcgggcgc agaccgagaa ctatcccgcc
     1141 aagccattgc gcctgatctc gcctttcccg ccgggcggga gcgtggatct cgtggcgcgc
     1201 atgatcgccg cgaagctgcc cgatttcctg ggacagcagg tcgtcgtgga caaccgcagc
     1261 ggggcctcgg gcaacatcgg gaccgagctg gtggcgcgct ccgcgcccga cggctatacc
     1321 ctgctcgcga acaccatccc gttcgtggcg aacgcgtttc tctacagcaa gctgccgtac
     1381 gacccactca acgatttcgc gccgatcatg caggtatcct cgtcgcccaa cgtgctggtc
     1441 gtgcatccgt cgctcccggt gcgctcggtg aaggagctgc tcgcgctcgc ccgatcgaag
     1501 ccgggcggcc tgaattacgg ctcggccggc cccgccacca acccgcacat cgccggcgag
     1561 ctgttcaact acctcggcaa ggtgaacatc gtcgtggtcc acttcaaggg cggcggcccg
     1621 gcgctggtcg cggcgatcag cggtgaggtc ggcatcgcgt tcaccaattt tgcggagacg
     1681 tccgaattcg tgaaggcggg gcggctgcgc gcgctcggcg tcaccagtgc gaagcgcaac
     1741 ccggccatgc cggacctgcc caccatcgcg gaagccgggc tgccgggcta cgaattcacc
     1801 acctggcacg gcatctgggc gcccaaaggc acgccgcgcg ccatcgtcgc gctgctgaac
     1861 gaccggctca agaagactat ggccgccccg gatcaaaaaa agcgattcga ggaccgggga
     1921 ctggacgtca tcgccagctc gcccgaggaa ttttcagctc acttgcagag cgaatacaag
     1981 aagtggggca gggtgatcaa ggaacggggc atgaaggccg actgagcgcc ggctttgagg
     2041 gagaagggat ggggatgagg gatgaggggg aaaccttcag gttcaaagtt ccaagttcaa
     2101 ggttccaggt ttaaaaacgg cgtgacttcg cgggcacgtg cggaaccgtt acaccgacga
     2161 gcgtgtcgcg cgtcaggatc ttcgcgagct gctcctcgct ccagccttcc gtcccctccg
     2221 ggcgcatcgg caccaccacg taacgcatgt ccgcattgga atcgtgcacg cgcacggtga
     2281 cgtggtcggg cagcacggtg ccgaactcct tcagcaccgc gcgcggctcg aacacgacac
     2341 gggagcggta gttgctgctc ttgtaccacg tgggcggcat gccgagcagc tcgcgcggat
     2401 agcacgagca cagagtgcag acgatgacgt tgtgcacgtt gggcgtgttc tcgaccgcga
     2461 tcaggtgggt ggcctcgagg tccacgccca tctcggcgca cgccgccttg ccgtccttga
     2521 ggagccgctt cttgaacgcg gggtcgatcc aggcgcgcgc gatcacccgg gagccgcggt
     2581 agggaaattc ctcctcgaac agctccatcc ggcgcctgac ctcctcggcc gtgatcacgc
     2641 cctttgcctc gagcagctcc tgcatcgccc ggctcatgat ttcatgctcc gaaggcggac
     2701 cttcgtcctc cgcggctgac ggtgcgtggt ggtcgcgccc gtgatcgtgc ccgtgtttgc
     2761 gatgcgcgta gtaatcgaag gaagaatcgc tcatgtggtt tcaccccgac tcgtccggat
     2821 cggccggctc tggccggccg gacgccggct cgagccagtg ctcgaaaatt tccatttcaa
     2881 cgacatcgcg cttcggaccg cggtaatccg gccacacgtg cttttgcggg aagcgcacgc
     2941 gatacagcac tttcttgggc ttgccttcga acccataggc gagctcctcg gggttccgga
     3001 aggtgccgca gatgcgctcg atcacgcctt gcttgccgcg gatgtaatag ggcgtgcgcc
     3061 ggtgccccgg cggaaagatg cgcttgacca ccacccgatc gcccacttca aagaggtgtc
     3121 cgggcgccgg ggtataggtg gacggaagcg gtttcgcgcc ccgcctgcgg ccaggccgcg
     3181 tcatcggccc gcccccttgt cgcccggcga tcgtgtcttg ccgggaacgt cgaagcgcgc
     3241 cagcacctcg gccatcttgg ccttgagctc cgcctcggtg atgagaccct tctcgagcag
     3301 caggttgcgc agcgccgagg tcgtgcgctg gaagtagtcg agtttgtagt actgctcgcc
     3361 caggtcctcg gcggcccggc gcaactcatc gacgagaacg atcttcttgc gggtgagggt
     3421 attgcggagc gcgttggcct gtctttccca gaacttcatg ccgtggtcgg tcgtgtcgat
     3481 cggcccggcc gcttcaccgc ccatgtcgtg aggtcccttg ttgaggggtc tatcgctcat
     3541 cgccaacctc ccccggagtc accgcgcggg aaaaattcac cgcctgcgcg attcagcata
     3601 gcacagtgct tcatccttct gccttcatcc ttccaccgcg cgggcttccg ggaccagatg
     3661 atggaatcca attatccccg atgatccaat gcagttaaaa tccagcttcg attcgcagcc
     3721 tgtcacggga gtcggaccat ggggatcaag cttcggggcg tgatgcaaag ccccgtcacg
     3781 ccgctcaagg aggacttcag tcccgacctg ccgacgttcg agcggctgct ggacttccac
     3841 gtgcgcacca gcggcaccgc catttcgtgg ccgcaccaca agggagagtc gctcaacctg
     3901 acgatcgagg agcgcaagcg tttcgccgag gtggcagtga agacggtcgc ggggcgcgtg
     3961 cccgtatcca tacacgtgag cgcgctgtcg gtggaggact cgctggacct cgcgcggcac
     4021 gccgagaaaa tcgaggcgga cgcgatcatc gccatcacgc cgtacttctg gaacccctcg
     4081 gtcgaggcta tctacgagca cttcgtgcgc atcggcaccg cggtcgacct gccgctcatc
     4141 gcctacaact ccccgagcta tctctcgggc atcgagttca ccggcgagct ggtcacgcgc
     4201 ctgctcgagc ggctgccgaa cttcatcgcg atgaaggagg cgagcttcaa cagcgagaag
     4261 ttcctcgaga tcgcgcgcgc ggcgctcgcg ctgcgcccgg acttcgcgct ggtcaccggc
     4321 gtcgaatacc tgctgccgtc ggttccgctc ggcggcgtcg ggtcctattc ctccgccggg
     4381 gcgatctgcc ccaacctctg caccgagctg ttcgagtctt gcgtcgccgc cgactggcca
     4441 cgcgcgcgcg agtgccagta caagctctcg caactgtggc tgctgttccg cgaccagtac
     4501 ccttcttcgc tcaagggcgg aatggtcatg atggggcgcc ccgtcgggcc cacacgccag
     4561 ccacttccca ccgccacccc ggagcgccgg gcctacatcc gcggccagct cgaggagctc
     4621 ggcatcctcc agaccgagcc gcatggatgg taagaacaag tcgaaaggcg gaaggcgaaa
     4681 ggcgaaagta agtgcagaac ggttttgacc tttacttccg actttcgcct ttcgactttc
     4741 gccttatgac acgccatggc taatttcgag ctgggactgg tccatacccc cgtgacgccg
     4801 ttcacgcacg agcggcgcat cgactacgac ctgtacgaga agctgatcgg gtttcacctg
     4861 cgcaacggcg cggactcgct ggcgctgccc atgcacgcgg gagaatccgt cagcttgagc
     4921 gacgaggagc gcaagacgct gctcgagttc gcgatcggaa aagtcaaggg gcgagtgccg
     4981 gtcatcgccc acgtgagcca gtcgggcacc ggcatggccg tagcgctcgc gcgccacgcc
     5041 gagcaggcgg gggcggccgc catagtggcg accgtcccgt attactggac accgccgccg
     5101 gcgatgctgg tcgagcattt cacgcagatc ggctccgcgg cgcgcctgcc gtttttcgtc
     5161 tacaactcgc ccgaggagat gggcggggtg agggtgacca ccgagattgc gctgaagctc
     5221 atcgagcggc tcgacaactt cgccggcgtc gtggactcga gcctcgactg gcagttcatg
     5281 atcgaggtca tttcggtcgc gcggcgcaag cgcgcctcgt tccagctgct gtcgggaacg
     5341 gagtacatga tctcggccgg cgcgatcggc tcgacgggcg tgttcgcgcc gcttgccggt
     5401 atcgccccga aggtcgtgcg cgggctctac gagctctgcc gcgaggagcg ctatggcgac
     5461 gggcgcagaa accaggaagc gatcgcggca ctgcgccaag cggtgaagcg cgacggtgtt
     5521 gccggattga agggcgcgat gcgcgcgatg gggcgcgatt gcggcgggcc gcggccgccg
     5581 ctccggccgc tgggcgaggt cgagtatggt gcgctcgctg agcggctcgg tgccctcgcg
     5641 ttcctgagcg ccgaatccaa aggctggtga ccccactgag cccgtgacag cgggagagcg
     5701 tcagggcgcg agggcgaaca ctcgatttcc cgatctcacg atctcccgat ttcacggatg
     5761 agtcgaccca caacccacga tccacgcatg acgaaagaaa gacgagcggc attcgtgact
     5821 ggcgcgtcgc aaggcatcgg cgccgcgatc gctgtagctc ttgcgcgcga cggattcgac
     5881 gtcgcggttt cctcgacgcg tcccgagaag ctctcgggcg tcctcacgga tatcggggcg
     5941 gcgggcgggc gctcggcgcc ggtggcgctc gacgtgcgct cgcaggcgag catcgagcag
     6001 gcgatggcgc atgtcgtgag cgccttcggc catctcgacg tgctggtcaa caacgcgggc
     6061 gttccgctga agaagccggc gctggaggtc agcggcgcgg aatgggatgc ggtgatcggg
     6121 accaatctca ccggcacttt cttcatgagc cagcagatgg gccggcacct ggtgaaggaa
     6181 gggcggccgg gctgcatcat cagcatcgcc tcgacccacg gactggtggg attggcggga
     6241 cgctcgatct acgggatcgc caaggcgggg atgatccaca tgacgcgcat gctggcgata
     6301 gagtgggcgg agcacggcat cagcgtcaac gcgatcgcgc cgggccgggt ggacacgcca
     6361 tcgcgcgcgg gatcgctcgc cgagcccggc taccgggacg cggccctcgc gcggataccg
     6421 ctcaagcggt tcgccaccgc cgaagaaatc gcagcggctg tgtgctacct cgtgagcccc
     6481 gcggcgacgt acatcaccgg gcagacgctg gtcctcgatg gcgggctgac cgccgcctga
     6541 tccgcgctgc aatagcgcgg gatgtaggtc atttcttgcc tgttcatcgc agcaagcccg
     6601 caaaccttga tttagcgcaa agtttgtagc gccacgattc gctacatt
//
LOCUS       DBNGWZ010000299         4797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_285566, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000299 DBNGWZ010000000
VERSION     DBNGWZ010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_285566"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="AC16CX_08735"
     CDS             complement(<1..421)
                     /locus_tag="AC16CX_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3012633.1"
                     /translation="MSIQITKDDGIATVTLDRPDKLNALSEEMYRGIADRFSALDADD
                     GVRAIVLTGAGRAFCAGGDMGSMGGYDVVSGRKRSQKHKRTVVNVYNVEKPVIAAVRG
                     PAAGIGFSLALACDLIIASESAYFLAAFKKVGIPPDGG"
     gene            complement(505..1503)
                     /locus_tag="AC16CX_08740"
     CDS             complement(505..1503)
                     /locus_tag="AC16CX_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012634.1"
                     /translation="MNEPHHPRSATRDTRRALIFLATLALASGALCAQSYPSKPVRIV
                     VPFPAGGPTDIVARALAQKMTQGLGQSVVVENRAGAGGVLGTELVARSPADGYNLLMG
                     TIGGLAVSMSLLPNRGYDTLRDFAPITQAVNVTNFLVTHPSVPAKSVKELLALARTRP
                     GRLNYASSGNGTVTHLAGELLKLMGGVNIVHVPYKGGAPALTALVSGETDISFENALI
                     IVPQVRSSKVRALAVTGSKRSPLLPDLPTIGETLPGYAASGWYGLVAPAATPRDALAK
                     LHAAAVKALRAADVVERLSSQGAEPVGTSPEEFAAFIRSEIDKWANLVKVTNMKPE"
     gene            complement(1506..3671)
                     /locus_tag="AC16CX_08745"
     CDS             complement(1506..3671)
                     /locus_tag="AC16CX_08745"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3012635.1"
                     /translation="MTRPERQHRTTRLDLSRFLNPRGVAVVGVSSDPTRIGGQALKLL
                     TDYGFKGAIYPVNPKYPKVGGLACYPDLVAVPQPCDVALIALAAHHVPGAIEQCGRAG
                     IPFALVLSSGFSEVGGAGKALQEKLLSAARKAGVRVVGPNCLGIINLRDNARIGFGGT
                     SQLTTLKAGPMAMVTQSGGFGFGVMSAASYYGVGCNYAISTGNEADLTLLDWVADLIE
                     RPEVETVVAFMEGVDDGRRLVEIGERALELGKPVLAWKVGNTDIGSRAATSHSARMTA
                     GYELYRAAFKRSGLVEIRDVDDLIDFAKAFRMHKLPAGNRVGVLTLSGGAGVLLADRC
                     IEHGLALPALSDATREKLSKTLVSFASFNNPVDATAHGYNDNFASYGEAVRLVLADPN
                     IDQVVARVPRGTSARPWSENLLKTLRGTDKPLILNWPTAPDDNGDVLTWLEENGVPCI
                     LGAGRAVHALAALTDFAQKQRARQERAKRPFERVIARQALDLPGKAGTLGEHRSKQLL
                     KRYGVPVVNEALLQPSEIEALTSSPLGFPAAVKIESPDVPHKTEAGAIRLDIRDLAGL
                     KQAAREVISAAKKYKQDARIEGVLVQEMTSGTEVIVGAVNDPYFGPTVAFGLGGIFTE
                     LIKDVTHRFAPFDAAAAREMIAEVKCAALLTGYRGRPPLDVDALADALARVSLLIADH
                     ADRIAEIDVNPLFVREQGVAAADALIVLKDSTQSRKGEK"
     gene            complement(3682..4494)
                     /locus_tag="AC16CX_08750"
     CDS             complement(3682..4494)
                     /locus_tag="AC16CX_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3012636.1"
                     /translation="MTMTATRPVTRSIVERGEVRIEVLTQGDGPPVVLIPSLGRGAED
                     FDDLARRLAAAGYRALRPQPRGIGRSSGPMKGITLHDFARDVAAVIEKNGGGPAVVTG
                     HAFGNFVARTTAADFPQRVKAVILLGATHVWPVPPDVRASINKSHDMSLPEEERIHCM
                     KHAFFAPGNDPRVWLGGWHEEVMHAERAATEATPKEEWWFAGRAPVLDMQPARDVMTP
                     PESRNRYRDEFGAERVTMTVIPNAGHALLPEQPEAVAAAILAYLKRLGLHDS"
     gene            complement(4505..4735)
                     /locus_tag="AC16CX_08755"
     CDS             complement(4505..4735)
                     /locus_tag="AC16CX_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024802.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3012637.1"
                     /translation="MFELKTRTSVDLLDNEAEALGAASFGIGHVAIGVALGYIDFRFP
                     ELGWREGHPKIAAWYESCAQRPSARSTMPVDE"
BASE COUNT          769 a         1582 c         1614 g          832 t
ORIGIN      
        1 cgccgccgtc gggcgggatg ccgaccttct tgaacgcggc gaggaaatag gccgattcgg
       61 acgcgatgat gaggtcgcag gcgagggcca ggctgaagcc gatgcccgcc gcgggcccgc
      121 gcaccgcggc aatcaccggc ttctcgacgt tgtagacgtt tacaacggta cgcttgtgct
      181 tctgcgagcg cttgcgcccg ctgacgacgt cgtaaccgcc catcgagccc atgtcgccgc
      241 cggcgcagaa ggcgcgtccg gcgccggtca gaacaattgc gcgcacgccg tcgtccgcgt
      301 cgagcgcgct gaaccggtcg gcgatgccgc gatacatctc ctcggacagc gcgttcagct
      361 tatccgggcg atcgagggtg acggtggcga tgccgtcgtc tttggtgatt tggatgctca
      421 tgaagtcagc attcagcaat cagcattcag catcagcatg aaccgtgagt catcgtagag
      481 tggctgatcg ctgaaggctg atcgctattc aggtttcata ttggtgacct tgacgaggtt
      541 agcccacttg tcgatttccg accggatgaa cgccgcgaat tcctcggggc tggtgccgac
      601 cggctcggcg ccctggctcg acaaccgctc gaccacgtcg gccgcccgca gcgccttgac
      661 cgcggcggca tggagcttcg cgagcgcatc cctcggcgtg gcggcgggag cgaccagccc
      721 gtaccagccg ctcgcggcgt aaccaggaag cgtctcgccg atggtgggga gatccggcag
      781 cagcggagag cgcttcgaac cggtaacggc caacgccctg accttgctcg accgtacctg
      841 cggaacaatg atgagcgcgt tctcgaagct tatgtcggtt tcgccgctca ccagcgccgt
      901 cagcgccggc gcaccgccct tgtacggcac gtgcacgatg ttgacgccgc ccatgagctt
      961 gagcagttcg cccgcgagat gcgtcaccgt gccgttgccc gatgaagcgt agttgagcct
     1021 gcccggccgt gtgcgcgcga gcgcgagcag ctccttcacc gatttcgcgg gcacggacgg
     1081 atgcgtgacg agaaagttag tgacgttgac cgcctgggtg atcggcgcga agtcgcgcag
     1141 cgtgtcgtag ccgcggttcg gcagcaggct catcgagacg gcgagtccgc cgatcgtccc
     1201 catcagcagg ttgtagccgt ctgcgggcga cctcgcgacc agctcggtgc ccagcacgcc
     1261 gccggcgccg gctcggttct cgaccaccac ggactgtccc agcccctgcg tcattttctg
     1321 cgcgagcgcc cgcgcgacga tatccgtcgg cccgcccgcc gggaagggga cgacgatccg
     1381 caccggcttc gaaggatacg attgggcaca taaagcgccc gacgccagcg cgagcgttgc
     1441 gaggaatatc agggcccggc gcgtatcgcg ggtcgcggat cgtggatggt gcggttcgtt
     1501 catgttcact tttcaccttt gcggctttgc gtcgagtcct tcaggacgat gagcgcgtcg
     1561 gcggcggcga cgccttgctc ccgcacgaag agcggattca cgtcgatctc ggcgatgcgg
     1621 tccgcatggt cggcgatgag cagcgacacg cgcgccagcg catcggcgag cgcgtccacg
     1681 tcgagcggcg ggcgtccgcg gtaaccggtg agcagcgccg cgcacttgac ctcggcgatc
     1741 atctcgcgcg cggccgcggc gtcgaacggc gcgaagcggt gcgtcacgtc cttgatgagc
     1801 tcggtgaaga tgccgccgag gccgaaggcg acggtcggtc cgaaataggg atcgttcacc
     1861 gccccgacaa tgacttcggt tccagaagtc atttcctgta ccagtactcc ttcaatacgg
     1921 gcatcctgct tgtatttttt cgctgcggag atgacttccc gcgccgcctg cttcaggccc
     1981 gcgaggtccc ggatgtcgag cctgatcgcc cctgcttcgg tcttgtgcgg cacgtcgggc
     2041 gattcgatct tgacggcggc ggggaagccg agcggagacg acgtgagcgc ctcgatttcg
     2101 gaaggctgga gcagcgcctc gttcaccacc ggcaccccgt agcgcttgag aagctgcttg
     2161 gagcggtgct cgcccagcgt tccggctttc ccggggagat cgagcgcctg gcgtgcgatg
     2221 acgcgctcga aggggcgctt ggcgcgctct tggcgggcgc gctgcttttg cgcaaaatcg
     2281 gtgagcgcgg caagggcgtg caccgcgcgc ccggcgccca ggatgcaggg aacgccgttt
     2341 tcctccagcc aggtcagcac atctccgttg tcgtccggcg cggtcggcca gttgagtatg
     2401 agcggcttgt cggtgccgcg cagcgtcttc agcaggtttt ccgaccaggg acgcgccgat
     2461 gtgccgcgcg gcacgcgggc gacgacctgg tcgatgttgg gatcggccag caccaggcgg
     2521 acggcctcgc cgtaagaagc gaagttgtcg ttgtagccgt gcgcggtggc gtccaccggg
     2581 ttgttgaacg acgcgaacga gaccagcgtc ttgctcagct tctcccgggt ggcatcggac
     2641 agggcgggga gcgcgagacc gtgctcgatg cagcggtccg cgagcagcac gcccgcgcca
     2701 cccgagagcg tgaggacgcc cacgcggttg ccggcgggca gcttgtgcat gcgaaaagcc
     2761 ttggcgaaat cgatgagatc gtccacgtcg cggatctcga ccaggccgct gcgcttgaac
     2821 gctgcgcggt agagctcgta gcccgcggtc atcctggccg agtgtgaggt agcggcccgg
     2881 ctgccgatat cggtgttgcc gaccttccac gcaaggaccg gcttgccgag ctcgagtgcg
     2941 cgctcgccga tctcgaccag ccggcggccg tcgtcgaccc cctccatgaa ggcgacgacg
     3001 gtttccacct cggggcgctc gatgagatcg gcgacccagt cgagcagcgt caaatcggct
     3061 tcgttgccgg tggagatcgc gtaattgcag ccgacgccat agtaggaggc ggcggacatg
     3121 accccgaagc cgaagccgcc gctctgcgtg accatcgcca tcgggcccgc cttgagcgtc
     3181 gtcagctgcg aggtgccgcc gaagccgatg cgggcgttgt ccctgagatt gatgatgccg
     3241 aggcaattgg gaccgaccac gcgcacgccg gccttgcgcg ccgcggagag gagcttctcc
     3301 tgcagcgcct ttcctgcccc gcccacttcg ctgaacccgc tggagagcac gagcgcgaac
     3361 gggatgcccg ctcttccgca ttgctcgatg gccccgggca cgtggtgggc ggcgagcgcg
     3421 atcagcgcga cgtcacacgg ttgcggtacc gccacgagat cgggataaca ggcgagtccc
     3481 ccgaccttgg ggtacttggg attgaccggg tagatcgcgc ccttgaagcc gtaatcggtg
     3541 agcagcttca atgcctggcc gccgatgcgc gtgggatcgc tcgatacgcc gacgaccgcc
     3601 accccgcgtg gattcaagaa gcgcgacaga tcgaggcggg ttgtccggtg ttgcctttca
     3661 ggtctagtca cgagtcacca gtcacgagtc atgcaatccg agccgcttta gataagcgag
     3721 gattgccgca gcgacggcct ccggctgctc gggcagcaag gcgtgcccgg cgttcgggat
     3781 cacggtcatc gtcacgcgct ccgcgccgaa ttcgtcgcgg taccggttcc tgctctcggg
     3841 cggagtcatc acgtcccggg cgggctgcat gtcgaggacc ggagcgcggc cggcgaacca
     3901 ccattcctcc ttcggcgtcg cttcggtggc ggcgcgctcg gcgtgcatca cctcctcgtg
     3961 ccagccgccg agccagactc gtgggtcgtt gccgggcgcg aaaaacgcgt gcttcatgca
     4021 gtggatgcgc tcctcctcgg gcagcgacat gtcgtggctc ttgttgatgg aggcacgcac
     4081 atcgggcggc accggccaca cgtgcgtcgc cccgagcagg atcacggcct tgacccgctg
     4141 cgggaaatcg gcggccgtcg tgcgcgcgac gaaattgccg aaagcatggc cggtcaccac
     4201 cgccgggccg ccgccgttct tctcgatcac cgcggcgacg tcgcgcgcga aatcatgcaa
     4261 ggtaatgccc ttcatggggc cactgctcct gccgatgccc cgcggctgtg gtcgcagtgc
     4321 gcgatagccc gcagccgcaa ggcgccgcgc gagatcatca aagtcctcgg cgccacgccc
     4381 cagcgaggga ataagcacga ccggtgggcc gtcaccctgc gtcaggactt cgatgcgcac
     4441 ctcgccgcgc tcgacgatgc tgcgagtgac cggtcgcgtt gcagtcatgg tcatggcgcg
     4501 cccgctattc gtcaacgggc atggtcgacc gcgctgaagg ccgttgcgcg caggattcgt
     4561 accacgccgc gatcttcggg tgaccttcgc gccagcccag ctcggggaag cggaagtcga
     4621 tgtaacccag ggcaacgccg atggcgacgt gcccgattcc gaacgaggcc gcgccgagcg
     4681 cctcagcttc gttatccagg agatccacgc tggttcgggt cttcagttca aacatcaccc
     4741 atcactcatc accgcccctg aaacttgggt ggccttttct ccctgaacgc gtccacc
//
LOCUS       DBNGWZ010000300        15061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_410040, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000300 DBNGWZ010000000
VERSION     DBNGWZ010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_410040"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..875
                     /locus_tag="AC16CX_08760"
     CDS             69..875
                     /locus_tag="AC16CX_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012638.1"
                     /translation="MENLVEIKDVNFSYDVRPVLKGINMTVPRGKIVAIMGLSGCGKT
                     TLLRMIAGVLKPRTGTVKVAGRVMRELDQEALYAMRRKMGMLFQFGALFTDMSVFDNV
                     AFQMREHTDLPEPVIQDLVLMKLQAVGLRGAQHLMPSELSGGMARRVALARAIALDPM
                     LIMYDEPFTGLDPISMGVISNLTRQLNNALGATSIIVTHDVQESLKIVDYVYFMSEGM
                     VVAQGTPKDIRNSATPFVHQFVWGEMDGPMPFHYPGASYAGDLELEVARA"
     gene            883..1665
                     /gene="mlaE"
                     /locus_tag="AC16CX_08765"
     CDS             883..1665
                     /gene="mlaE"
                     /locus_tag="AC16CX_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HIR3012639.1"
                     /translation="MRGLRGVGHQVVNGVWRLGYATRFFLLTLLRSGVSFRRFRGLTF
                     REIYFAGVLSLIIIIVSGLFVGMVLGLQGYETLQKYGSSEAVGTLVALSLTRELGPVV
                     AALLFASRAGSAMTAEIGLMKATEQISAMEMMAVDPIARVIGPRFWGGVISMPLLAAI
                     FSAVGVFGGYLIAVVLIGVDEGAFWSQMQNAVDFREDIVNGVIKSVVFGVAVTAISLF
                     EGYDAPPTAEGVSGATTRTVVTSSLAILGLDFILTAFMFKGV"
     gene            1670..2200
                     /gene="mlaD"
                     /locus_tag="AC16CX_08770"
     CDS             1670..2200
                     /gene="mlaD"
                     /locus_tag="AC16CX_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HIR3012640.1"
                     /translation="MDRTVLDAWVGIFVVVGIAALFILAFQVSNATFSRGDAYRITAE
                     FDNIGGLKVRAPVKSAGVVVGRVGEIQFDSKKFVARVILTIDKRYEFPKDTSVSILTS
                     GLLGEQYIGLEAGGDTVVLKDGDTIKLTQSAVVLEKLIGQFLYNKAAEGGAKPAEGGA
                     KPAEGGAKPAEGGATK"
     gene            2228..2887
                     /locus_tag="AC16CX_08775"
     CDS             2228..2887
                     /locus_tag="AC16CX_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3012641.1"
                     /translation="MKRINDLMMKKLLMSLLAASFLMAVPVRSAETVAPDALAKSVTE
                     EVLVVLRSDKDIQAGNVWKVVDLVEKKVLPHFEFVRMTRLAVGVNWRQASPEQQKTLV
                     NEFRALLVHTYAATFVAYRNQTIDYRPLRMQPNDTEVVVKSLINQPGGKPVTVDYKME
                     KTGNGWKVYDVVVGDLSLVQNYRGSFETEVRKGGIDGLIKALVDKNKQLAADQPSAAK
                     Q"
     gene            2884..3165
                     /locus_tag="AC16CX_08780"
     CDS             2884..3165
                     /locus_tag="AC16CX_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3012642.1"
                     /translation="MIECNGGRCTVQGPINLDSVVSLLAEGNGLFTAPQVTVDLSAVT
                     EVDSSALSLLLEWRREAGRNGREIRYLNLPANLKSLAELYGVTELLGEA"
     gene            3242..4141
                     /locus_tag="AC16CX_08785"
     CDS             3242..4141
                     /locus_tag="AC16CX_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3012643.1"
                     /translation="MVPAIQVERISKSYGALRALDEVSLTIAQGEFFGLLGPNGAGKT
                     TLINILAGLALATSGAARILGHDVFSQYRAARQALGVVPQELVFDPFFTVREALTFQS
                     GYFGLRNNRDWIEEVMHHLDLTDKSETNMRALSGGMKRRVLVAQALVHKPPVIVLDEP
                     TAGVDVELRQALWQFIRRLNRDGHTIVLTTHYLEEAEALCGRIAMLKQGRIVALDTKQ
                     NLLRMHTGCYLELRLHPDRLPERLEALVTRRREGGYRLALRDYNEVERVIAELRSTGV
                     EIRELEVMQPDLEEVFVQIMRKA"
     gene            4190..4981
                     /locus_tag="AC16CX_08790"
     CDS             4190..4981
                     /locus_tag="AC16CX_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3012644.1"
                     /translation="MNRTPLTPHPLPLTGFYTLFYKEWLRFWKVSVQTILAPVLTALL
                     FLVVFAYSLRGRVEIFPGVDYASFLVPGLAMMSVLQNAFANSSSSLMQSKMTGNIIFV
                     LLPPLSHQEFFSAYLLASIARGVVVGAGVFLATVWFVDLRFEHPLWAVVFAIAGSGVM
                     GALGIIAGIYSDKVDELAAFQNFIILPLTFLSGVFYSIQSLPPFWRWLSHLNPIFYMV
                     DGFRYGFFGQSDVSPWFSLAFVCASFFALSFFTVWLLRTGYKLRH"
     gene            5099..5341
                     /locus_tag="AC16CX_08795"
     CDS             5099..5341
                     /locus_tag="AC16CX_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR3012645.1"
                     /translation="MQVIDTDQIKGWIEGGLVCEHVEVRGDGHHFEALIVSPAFRGKS
                     RVQQHQLVYSALGGRMAGEIHALSMQTYTPEDWARR"
     gene            5354..6607
                     /gene="murA"
                     /locus_tag="AC16CX_08800"
     CDS             5354..6607
                     /gene="murA"
                     /locus_tag="AC16CX_08800"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3012646.1"
                     /translation="MDKLLIEGGAPLSGEIGVSGAKNAALPILTAALLTDEPLTVTNV
                     PHLRDVTTMLGLLAQMGVAVSMNVRLGVELAAARLVNPVAPYELVKTMRASVLVLGPL
                     TARCGEARVSLPGGCAIGLRPVDQHIKGLQAMGAELTIEHGYISVRAKRLRGARVCMD
                     LVTVTGTENIMMAATLAQGTTVIENAAREPEVADLANCLNAMGARVQGAGTDVITIEG
                     VERLHGAQHRVMPDRIETGTFLVAAAATGGEITLRGARPDILDAVLEKLRESGAVIET
                     GKDWIALRTNGALNAVNVRTAPYPAFPTDMQAQFMALNSTARGTALVTETIFENRFMH
                     VQELKRLGADIEVEGNTAVVKGVAHLDGAIVMATDLRASASLVLAGLIARGTTTVDRV
                     YHLDRGYESIEEKLSKLGARIRRVR"
     gene            6790..7425
                     /gene="hisG"
                     /locus_tag="AC16CX_08805"
     CDS             6790..7425
                     /gene="hisG"
                     /locus_tag="AC16CX_08805"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3012647.1"
                     /translation="MITIALSKGRIYDETAPLLKAAGIVARDDPEKSRKLILRTNRKD
                     VRLVIVRAADVPTYVQYGAADLGVAGKDVLDEHGGQGLYQPLDLGIARCRMVVAVPAG
                     FDYRRAVRQGARLKVATKYVQTAREHFAAKGVHVDLIKLYGSMELAPLTGLADAIVDL
                     VSTGRTLKANHLKAVEEIAPLSARLIVNQAALKLKRAAIQPLLDAFARAAR"
     gene            7422..8762
                     /gene="hisD"
                     /locus_tag="AC16CX_08810"
     CDS             7422..8762
                     /gene="hisD"
                     /locus_tag="AC16CX_08810"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3012648.1"
                     /translation="MMQIRRLATTQPDFDARLAELLAFEAAQDPQVEATVAAILADVK
                     ARGDAALLEYTRRFDRVEANSVTALEVPRAELGRALRRIPRTIRAALETAARRVRTYH
                     RKQLAASWRYRDAAGTELGQRVTALDRVGLYVPGGKASYPSSVIMNSVPAKVAGVGEI
                     IMVVPAPGGEKNDLVLAAAAICGVDRVFTIGGAQAVAALAYGTATIPAVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMMAGPSEVVVVCDGKTEADWVAMDLFAQAEHDELAQAILITP
                     DAAFIEAVAVSMTRQLDGMPRREVIAASFSKRGALIRVRNLNEACAVVNRIAPEHLEL
                     SVGNPDTLLPKIRHAGAIFLGRHSSEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSVIRVSRDAARKLGAVAAELARGEGLTAHARSAEYRMQKMNRQDAKKNKNNN"
     gene            8947..10020
                     /gene="hisC"
                     /locus_tag="AC16CX_08815"
     CDS             8947..10020
                     /gene="hisC"
                     /locus_tag="AC16CX_08815"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3012649.1"
                     /translation="MPKTPEEIIRDEIRALKGYRVPDATGMVKLDAMENPYPLPQALR
                     ARLARLVEETFLNRYPDSSGSRLRGLLRSAFEVPDGAELLLGNGSDELIQMVFTAAAK
                     PGAVVLGVEPSFVMFRIIATFAGARYVGVDLREDFSIDVERMLGAIEQHRPGVIIIAY
                     PNNPTGNLFDAGMIERIIEAAPGLVLVDEAYHAFAAKSFLPRLPRHPNLLVMRTLSKL
                     GLAGIRLGVLAGASRWLEQIGKVRLPYNVNTISQVVACEMLQHGDVLTEQAAAIKLER
                     GRLLEALRRLPGVTAHPSDANFILFKVRQAERVFDGLKQRGVLIRSLHGSHRLLADCL
                     RVTVGTPDENAAFLTALTETLAA"
     gene            10072..10656
                     /gene="hisB"
                     /locus_tag="AC16CX_08820"
     CDS             10072..10656
                     /gene="hisB"
                     /locus_tag="AC16CX_08820"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR3012650.1"
                     /translation="MRKAEITRDTKETKIGVKLDLDGRGRAKIATGVPFLDHMLEQVA
                     RHGAFDLEVSAKGDLRIDAHHTVEDIGITIGQAFAKAVGDKKGVRRYGHAYVPLDEAL
                     SRAVVDLSGRPGLEYHMEFTRARIGEFDVDLVHEFFQGFVNHAQVTLHLDNLKGTNAH
                     HQAETAFKAFARALRMAVEADPRVKGVPSTKGSL"
     gene            10771..11454
                     /gene="hisH"
                     /locus_tag="AC16CX_08825"
     CDS             10771..11454
                     /gene="hisH"
                     /locus_tag="AC16CX_08825"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3012651.1"
                     /translation="MTDVAVVDYGMSNVRSVALAIRRVAPQLKVSVTSDANVVNRAVR
                     VVFPGQGAMPDCMREMDARGLRGAVLEAARAKPFLGICIGLQMLFEASEEGDTPGLGL
                     LPGRVRRFPPEKMVDAEGARLKVPHMGWNEVRQTVPHPLWEGIAQASRFYFVHSYFPE
                     PGSRGLTAGESVYGVTFTCAAARDNVFAVQFHPEKSDAAGLRLLANFIGWQPDTQRAE
                     HSATASPAA"
     gene            11672..12433
                     /gene="hisA"
                     /locus_tag="AC16CX_08830"
     CDS             11672..12433
                     /gene="hisA"
                     /locus_tag="AC16CX_08830"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3012652.1"
                     /translation="MLIIPAIDLKDGKCVRLKQGAMDKATVFSDDPPAMAVQWLKLGA
                     RRLHVIDLNGAAAGRPKNEQAIKSITEAVGGKLPIQLGGGIRDLDTIEKLLDSGVSYV
                     IIGTAAVKTPGFLHDACTAFPGHVLVALDAKDGKVAVDGWSKMTGHEVLDLAKKFQDY
                     GVDAIIYTDIGRDGMMTGVNIAATVALAKELTVPVIASGGINGLEDVKALCKVEHEGI
                     IGAVTGRAIYEGKLDFAKAQKLADEFSKKNEGNGK"
     gene            12504..13274
                     /gene="hisF"
                     /locus_tag="AC16CX_08835"
     CDS             12504..13274
                     /gene="hisF"
                     /locus_tag="AC16CX_08835"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3012653.1"
                     /translation="MGLAKRIIPCLDVTAGRVVKGIHFVGLRDAGDPVEVARRYNEQG
                     ADELAFLDITASSDGRDLIVSVVEAVAEQVFIPLTVGGGVRSVADVQRLLNAGADKVS
                     INTAAVQNPRLVEEATARYGSQCIVVAVDAKRVPGPGAPRWEVYTHGGRRPTGLDALE
                     WGRRMEAAGAGEILLTSMDRDGTRSGFDLELTRAFSDALSIPLIASGGVGSLDHLAEG
                     VLQGRADAVLAASIFHYGEFTVRQAKEHLARRGIEVRL"
     gene            13376..13765
                     /gene="hisI"
                     /locus_tag="AC16CX_08840"
     CDS             13376..13765
                     /gene="hisI"
                     /locus_tag="AC16CX_08840"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HIR3012654.1"
                     /translation="MNESWLDKVKWAQDGLVPAVAQETGSGRVLTVAWMNREALGKTV
                     QTGEAHYWSRSRGKLWRKGEESGHAQKVRTIRLDCDEDVILLEVEQVGGIACHTGRHS
                     CFFRRLDGAQWVVTDPVLKDPKGIYKR"
     gene            13762..14082
                     /locus_tag="AC16CX_08845"
     CDS             13762..14082
                     /locus_tag="AC16CX_08845"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR3012655.1"
                     /translation="MINANILRRLGTVIAERKQSDPSASYVASLFAKGEDAILKKLSE
                     EAAETVLAAKGGDKLHIVRETADLWFHSLVLLAWHGLAPEDVLAELQRREGISGIDEK
                     KSRG"
     gene            14107..14337
                     /gene="tatA"
                     /locus_tag="AC16CX_08850"
     CDS             14107..14337
                     /gene="tatA"
                     /locus_tag="AC16CX_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HIR3012656.1"
                     /translation="MGSFSIWHWLIVLLVVVLIFGTKKLRNIGADLGSAVKGFKDGMK
                     SEEDKSAQAKAEIPPTTGQTIEGEAKKETPKA"
     gene            14422..14829
                     /gene="tatB"
                     /locus_tag="AC16CX_08855"
     CDS             14422..14829
                     /gene="tatB"
                     /locus_tag="AC16CX_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR3012657.1"
                     /translation="MFDIGFSELLVIAIVALIVIGPQRLPTVARTLGHLFGRMQRYVN
                     DVKADIAREMELEELKKMQASMEDAARSMRDSVTREVSETESELNRLAQAAQPAPDAY
                     SDAPPQEPAAKSASQQLDLALDQSANAPTQKQA"
BASE COUNT         2679 a         4649 c         5098 g         2635 t
ORIGIN      
        1 atcgtaattg ttgagcagga acgggtggtt taaaattagc ccctttttcg cgcctgaggg
       61 ccggcttgat ggaaaacctc gttgaaatca aggacgtcaa tttttcctac gacgtccggc
      121 cggtgctgaa ggggatcaac atgaccgtgc cgcgcgggaa aatcgtggca atcatgggcc
      181 tctccggctg cggcaagacc accctcctgc gcatgatcgc cggcgtgctg aagccccgca
      241 ccggaaccgt gaaggtcgcc ggccgcgtga tgcgcgagct cgaccaggaa gcgctttacg
      301 ccatgcgccg caagatgggc atgctgttcc agttcggggc gctcttcacc gacatgtcgg
      361 tgttcgacaa cgttgctttc cagatgcgcg agcacaccga tctgcctgaa ccggtgatcc
      421 aggacctggt gttgatgaag ctgcaggcgg tgggactgcg cggcgcccag cacctgatgc
      481 cctcggaatt atcgggcggc atggcgcgtc gcgtagcgct tgcgcgcgcc atcgcgctcg
      541 acccgatgct catcatgtat gacgagcctt ttaccggcct ggacccgatc tcgatgggcg
      601 tcatcagcaa cctgacccgg cagctcaaca acgcgctggg cgcgacttcc atcatcgtca
      661 cgcacgacgt gcaggaatcg ctcaagatcg tggactacgt gtatttcatg tcggagggaa
      721 tggtcgtggc gcaaggcacg ccgaaggaca tccgcaattc ggcgaccccg ttcgtgcacc
      781 agttcgtgtg gggcgagatg gacgggccga tgccgttcca ttacccgggc gcgtcgtacg
      841 cgggcgacct ggagctggag gtggcccgtg cctgagcgcg tcatgagggg gttgcgcggc
      901 gtcggccacc aggtcgtgaa cggcgtgtgg cggctcggct atgcgacccg ctttttcctg
      961 ctcaccctgc tgcgatcggg cgtgagcttc cgccgcttcc gcggcctcac cttcagagag
     1021 atctatttcg ccggcgtgct gtcgctcatc atcatcatcg tctcgggcct gttcgtgggg
     1081 atggtgctgg gcctgcaggg ctacgagacg ctgcagaaat acggctcatc cgaggcggtc
     1141 ggcacgctgg tcgcgctctc gctcacgcgc gagctggggc cggtggtcgc ggcgctgctg
     1201 ttcgcaagtc gcgccggctc ggcgatgacc gcggaaatcg ggctgatgaa ggcgaccgag
     1261 cagatctcgg cgatggagat gatggcggtc gatccgatcg cgcgcgtgat cgggccgcgc
     1321 ttctggggcg gcgtgatctc catgccgctg ctcgccgcga ttttcagcgc ggtgggggtg
     1381 ttcggtggct atctcatcgc ggtggtgctg atcggggtgg acgaaggcgc gttctggtcc
     1441 cagatgcaga acgcggtgga tttccgcgag gatatcgtga acggcgtgat caagagcgtg
     1501 gtgttcggcg tcgcggtgac cgccatctcg ctgttcgagg ggtacgacgc cccgcccacc
     1561 gcggaaggcg tgtcgggggc gaccacgcgc acggtggtca cctcgtcgct cgccatactc
     1621 gggctggatt tcatcctcac cgccttcatg ttcaagggag tctaggtcta tggacagaac
     1681 cgttctcgat gcttgggtcg gcatattcgt cgtcgtcggc attgccgccc tgtttatcct
     1741 tgccttccag gtgagcaacg ccacgttcag caggggcgac gcctaccgga tcaccgccga
     1801 gttcgacaac atcggcggac tcaaggtgcg tgcgccggta aaaagcgcgg gcgtggtggt
     1861 cggccgggtc ggcgagatcc aattcgacag caagaagttc gtcgcgcggg tgatcctcac
     1921 catcgacaag cgctatgagt ttcccaagga cacctcggtg tccatcctca cctcgggcct
     1981 gctcggcgag cagtacatcg gtctcgaggc ggggggcgac accgtggtgc tcaaggacgg
     2041 cgacacgatc aagctcacgc aatccgccgt ggtgctggag aagctgatcg ggcagttcct
     2101 ctacaacaag gccgcggaag gcggggccaa gcctgcggag ggcggggcca agcctgcgga
     2161 aggcggggcc aagcccgcgg aaggcggggc caccaagtag tgcccagtgc tgagtgctga
     2221 gtgacgagtg aaaaggataa atgacctcat gatgaagaaa ttgctgatga gtttgctggc
     2281 ggccagtttt ctgatggctg tgccggtgcg ttcggcggag acggtggcgc ccgatgcgct
     2341 tgcgaaaagc gtgacggagg aagtgctggt ggtgctgcgc tcggacaagg acatccaggc
     2401 aggcaacgtg tggaaagtgg tggacctggt cgagaaaaaa gtactgccgc atttcgagtt
     2461 cgtgcggatg acgcggctcg cggtcggcgt gaactggcgg caggcgagcc ccgagcagca
     2521 gaagacgctg gtcaacgagt tccgggcgct gctggtgcac acgtatgccg cgacgttcgt
     2581 ggcctaccgc aaccagacca tcgattaccg gccgttgcgc atgcagccca atgacacgga
     2641 agtagtggtc aagtcgctga tcaaccagcc aggcggcaag cctgtcaccg tggattacaa
     2701 gatggagaag accggcaacg gatggaaggt gtacgacgtg gtggtggggg acttgagcct
     2761 ggttcagaac taccgtggca gtttcgagac cgaggtgcgc aaaggcggca tcgacgggct
     2821 gatcaaggcg ctcgtcgaca agaacaagca gctcgccgcc gatcagccat cggccgcaaa
     2881 gcaatgatcg aatgcaacgg cggccgctgc accgtgcagg ggccgatcaa cctggacagc
     2941 gtggtatcgc tgctcgccga gggcaacggg ctgttcaccg ccccgcaggt gacggtcgat
     3001 ctctccgctg tcacggaggt ggattcctcc gcgttgagcc tcctgctcga atggcggcgc
     3061 gaggcaggcc gcaacggccg cgagatccgc tacctcaacc tgcccgccaa tctcaagagc
     3121 ctcgccgaac tctacggcgt caccgaattg ctaggtgagg cgtaaggcgt gaggggtgag
     3181 gcgggtcaat ccgccttgcc ggttttcgcc tagcgcctta cgcctaacgc ttcacgcacc
     3241 gatggttccc gcaatccagg tcgagcgcat ttccaagagc tacggcgcgc tgcgggcgct
     3301 cgacgaggtg agcctgacga tcgcgcaggg cgagttcttc gggctactcg gaccgaacgg
     3361 cgcgggcaag accacgctca tcaacatcct tgccggcctt gcgcttgcca cgagcggcgc
     3421 cgcgcggata ctgggccacg atgtatttag tcagtatcgc gccgcgcggc aggcgctggg
     3481 cgtggtgccg caggagctgg tgttcgatcc gttcttcacg gtgcgcgagg cgctcacgtt
     3541 ccagtccggc tacttcggcc tgcgcaataa tcgcgactgg atcgaggagg tgatgcacca
     3601 cctcgacctg accgacaagt cggagaccaa catgcgggcg ctgtccggcg gcatgaagcg
     3661 gcgggtgctg gttgcgcagg ccctggtgca caagcccccg gtcatcgtcc tcgacgagcc
     3721 gaccgcgggc gtggacgtcg agctgcggca ggcgctgtgg cagttcattc gcaggctcaa
     3781 ccgtgatggc cataccatcg tgctcaccac gcattacctg gaggaggcgg aagcgctgtg
     3841 cgggcgcatc gccatgctca agcaggggcg catcgttgcg ctcgacacca agcaaaacct
     3901 gctgcgcatg cacacgggct gctaccttga gctgcggctt catccggacc ggctgccgga
     3961 gaggctcgaa gcgctggtca cgcgccgccg cgagggcggt taccgtctcg cgctgcgcga
     4021 ctacaacgaa gtcgagcgcg tgatcgccga actgcgatcg accggcgtcg agatccggga
     4081 gctggaagtg atgcaacccg acctggaaga ggtgttcgtc caaatcatgc gcaaggcgtg
     4141 ataggtgata agtgataggt gataggtatt gtgcgaatcg cggacgacga tgaatagaac
     4201 acccctcacc cctcacccct tacccctcac ggggttttac acgctgttct acaaggagtg
     4261 gctgcgtttc tggaaggtca gcgtgcagac gatactggcg ccggtgctca ccgcgttgct
     4321 gttcctggtc gtgttcgcgt actccctgcg cgggcgcgtc gagattttcc ccggggtgga
     4381 ctacgcgtct ttcctggtgc cggggctggc gatgatgtcg gtgttgcaga acgcattcgc
     4441 caacagctcc tccagcctga tgcagtcgaa aatgaccggc aacatcatct tcgtgctgct
     4501 gccgccgctg tcgcaccagg agttcttctc ggcttatctg ctcgcctcca ttgcgcgcgg
     4561 ggtggtcgtg ggcgcgggcg ttttcctggc caccgtctgg ttcgtggacc tccggttcga
     4621 gcatccgttg tgggcggtcg tcttcgcgat cgcgggcagc ggcgtgatgg gggcgctcgg
     4681 catcatcgcc ggcatctatt cggacaaggt ggacgagctc gcggcgttcc agaatttcat
     4741 cattttgccc ctcacgttcc tgtccggcgt gttctactcg atccagtcgc tgccgccgtt
     4801 ctggcggtgg ctgtcgcatc tcaatccgat cttctacatg gtggacggct tccgctacgg
     4861 ctttttcggg caaagcgacg tctcgccctg gttctcactc gcatttgtgt gcgcgagttt
     4921 tttcgcctta tcatttttca ccgtctggct gctgcgcacg gggtataagc tgcgtcactg
     4981 acagtaagag cgtgaaccgt gagccgtgaa ccgtgagccg tgaatcgatg gagccctttt
     5041 tgccagcggg aagttgtcgg atcacggatc acagattacg gctcacgcct ttcggaaaat
     5101 gcaagtgatc gacactgatc aaatcaaagg ctggatcgaa ggcgggctcg tctgcgagca
     5161 cgtggaagtg cggggcgacg gccatcattt cgaggcgctg atcgtgagcc cggcgttccg
     5221 cggcaagtcg cgcgttcagc agcaccagct ggtgtactcg gcgctcggcg gtcgcatggc
     5281 gggcgagatt cacgccctgt ccatgcagac ctatacgccg gaagactggg cccggcgatg
     5341 aggccggcac ggcatggata agcttctgat cgaaggcggc gcacccctct ccggcgagat
     5401 cggcgtgtcc ggcgccaaga acgcggcgct gcccatcctc accgccgcgc tgctcaccga
     5461 cgagccgctc acggtcacca acgttccgca cctgcgcgac gtgaccacga tgctcggcct
     5521 gctggcgcag atgggcgtcg cagtctcgat gaacgtaagg ctcggcgtcg agctcgccgc
     5581 cgcgcgcctc gtgaatcccg tggcgccgta cgagctggtg aagaccatgc gcgcctcggt
     5641 gctggtgctg gggccgctca ccgcgcgttg cggggaggcg cgggtgtcgc tgcccggcgg
     5701 ctgcgccatc gggctgcggc cggtggacca gcacatcaag ggtctgcagg cgatgggggc
     5761 ggagcttacg atcgagcacg gctacatttc ggtgcgggcg aagcgcctga ggggcgcacg
     5821 cgtctgcatg gatctcgtca ccgtcaccgg caccgagaac atcatgatgg ccgcgaccct
     5881 cgcgcagggc acgacggtga tcgagaacgc ggcgcgcgag ccggaggtgg ctgatctcgc
     5941 caactgcttg aacgccatgg gcgcccgggt gcagggtgcg gggaccgacg tgatcacgat
     6001 cgagggcgtg gagcggctgc acggcgcgca gcatcgcgtg atgccggacc gcatcgagac
     6061 cggcacattc ctggtggcgg cggccgcgac gggcggcgaa atcacgctgc gcggcgcgcg
     6121 gcccgacatc ctcgacgcgg tgctggagaa gctgcgcgag tcaggcgcgg tcatcgagac
     6181 cggcaaggac tggatcgcgc tcaggaccaa cggcgcattg aatgcggtca acgtgcgcac
     6241 cgcgccgtat cccgcgtttc ccaccgacat gcaggcgcag ttcatggcgc tgaacagcac
     6301 cgcgcgcggt accgccctgg tgaccgagac catcttcgag aaccgcttca tgcacgtgca
     6361 ggagctgaag cgcctcggcg ccgatatcga ggtcgagggc aataccgcgg tggtgaaagg
     6421 agtcgcgcat ctcgacggcg cgatcgtgat ggcgacggac ctgcgcgcct ccgcgagcct
     6481 ggtgctggcg ggtctcatcg cccgcggcac caccaccgtg gaccgcgtct atcacctcga
     6541 ccgcggctac gagagcattg aggaaaagtt gtcgaagctc ggcgcgcgca tcaggcgcgt
     6601 tcgttgaaag tccatgggtt gcactttccc gcgcgttgaa tggcgaggat tcgtccgttc
     6661 gaacgagcgg agttaaaccg gcgtgcagcg tccgggtttg acttcacttg gcgttcttgg
     6721 cgcctagtca gctgatttgg ggtctttgcg gtaaaatcct gtttttattg aatcattcgg
     6781 gttgcagacg tgatcacgat cgcgctctcc aagggccgca tctacgacga gaccgcgccg
     6841 ctgctgaagg cggcggggat cgtcgcacgc gacgatccgg aaaagtcgcg caagctgatc
     6901 ctgcgtacca atcgcaagga cgtgcggctc gtcatcgtgc gcgcggccga cgtgccgacc
     6961 tacgtgcagt acggcgcggc ggacctcggc gtcgccggca aggacgtgct cgacgagcac
     7021 ggcgggcagg gcctctacca gccgctcgac ctcggcatcg cgcgctgccg catggtggtc
     7081 gcggtgccgg cgggcttcga ttaccggcgc gcggtgcggc agggcgcgcg cctcaaggtc
     7141 gccaccaaat acgtgcagac ggcgcgcgag cacttcgccg ccaagggcgt gcatgtcgat
     7201 ctcatcaagc tctacggctc gatggagctt gcgccgctca ccgggctcgc cgacgcgatc
     7261 gtggacctgg tgagcaccgg caggacgctc aaggcgaacc acctgaaggc ggtcgaggag
     7321 atcgcgccct tgagcgcgcg cctcatcgtc aaccaggcgg cgctgaaact gaagcgcgcc
     7381 gcaatccagc ccttgctcga tgctttcgcg cgggcggcgc gatgatgcag attcggcgcc
     7441 tcgccaccac tcagcccgat ttcgacgcgc ggctcgcgga gttgctcgcg ttcgaggccg
     7501 cgcaggaccc gcaggtcgaa gccacggttg cggcgatcct cgccgacgtc aaggctcgcg
     7561 gggatgcggc gctgctggag tacacgcgac gctttgaccg cgtggaagcg aactcggtga
     7621 cggcgctcga ggtgccgcga gcggagctcg ggcgggcgct gcggcgcatc ccccggacga
     7681 tccgcgccgc gctcgaaacc gcggcgcggc gggtgcgcac ttaccacaga aagcagcttg
     7741 cggcctcctg gcgctatcgc gacgctgccg gcaccgagct gggccagcgc gtgactgcgc
     7801 tcgaccgcgt cggcctctat gtgccgggcg gcaaggcgtc ttatccgtcg tcggtgatca
     7861 tgaattcagt gccggcgaag gtggccggcg taggcgagat catcatggtg gtgccggcgc
     7921 ccggcgggga gaagaatgat ctggtgctcg cggccgccgc gatctgcggc gtggaccgcg
     7981 tgttcacgat cggcggcgcg caagcggtcg ccgcgctcgc ctacggcacc gcgacgatcc
     8041 ccgccgtgga caagatcgtg ggcccgggca atgcctacgt cgcggccgcc aagcggcgcg
     8101 tgttcggggt ggtcggcatc gacatgatgg cgggtccatc ggaagtggtg gtggtatgcg
     8161 acggcaagac cgaggccgat tgggtggcga tggacctctt cgcgcaggcg gaacacgacg
     8221 aactggcgca ggcgatcctg atcacgcccg atgccgcctt catcgaggcc gtcgccgtca
     8281 gcatgacgcg ccagctcgac ggcatgccgc ggcgcgaggt gattgccgcc tcgttctcga
     8341 agcgcggcgc gctgatccgc gtgcgcaatc tcaacgaagc gtgcgcggtg gtcaaccgca
     8401 tcgcgcccga gcatctggag ctgtcggtcg ggaatcccga caccctgctg ccgaagatcc
     8461 ggcacgccgg cgcgatcttt ctcgggcgtc acagctcgga agcgctcggc gactactgcg
     8521 ccggcccgaa ccacgtcctg ccgacctcgc gcaccgcgcg tttctcctcg ccgctcggcg
     8581 tctacgattt ccagaaacgc tcgagcgtga tccgcgtctc gcgcgacgcg gcgcgcaaac
     8641 tcggcgccgt cgctgccgag ctcgcgcgcg gcgaggggct gaccgctcac gcccgctcgg
     8701 ccgaataccg catgcagaaa atgaaccgcc aagacgccaa gaaaaacaag aacaataatt
     8761 gaaccgcaga gacgcggagc gaagccaaga caggaacgat gctaattgcg agaaccgccc
     8821 gttatatgag tctatttcat tcttgttctt ggttttcccg ttgtctcggt ggttcgatgg
     8881 ggtttgatat tcggttctat ttctgtattc gtttctctcg gcgccttggc ggttgattgg
     8941 ctttccatgc cgaaaacacc cgaagagatc atccgcgacg agatccgggc gctgaaaggc
     9001 taccgcgttc cggacgcgac gggcatggtg aagctcgacg cgatggagaa cccgtatccg
     9061 ttgccgcagg cgctgcgtgc ccggctcgcg cggctggtgg aggagacctt tctcaaccgc
     9121 tatccggatt cttcgggaag ccggttgagg gggctgctcc gcagcgcgtt cgaagtgccg
     9181 gacggagcgg agctgctgct gggcaacggt tccgacgaat tgatccagat ggtattcacg
     9241 gcagccgcga agccgggagc ggtcgtgctc ggcgtcgagc cttcgttcgt gatgttccgt
     9301 atcatcgcga ccttcgccgg ggcgcgttac gtcggcgtgg acctgcggga ggacttctcg
     9361 atcgacgtcg agcgcatgct gggcgctatc gaacagcacc ggccgggggt catcatcatc
     9421 gcctatccca acaatccgac cggcaacctg ttcgacgccg gaatgatcga gcggatcatc
     9481 gaggccgccc ccgggctggt gctggttgac gaggcctatc acgcattcgc cgcgaagagc
     9541 tttctgccgc gccttccccg ccatccgaac ctgctggtga tgcgcaccct gtcgaagctg
     9601 gggctcgcgg gcatccggct cggcgtgctg gccggcgcga gccgctggct cgagcaaatt
     9661 ggcaaggtgc ggttgccgta taatgtcaac acgatctcgc aggtcgtcgc gtgtgaaatg
     9721 ttgcagcacg gggacgtgtt gacagaacag gccgcggcca tcaagctaga gcgtgggcgc
     9781 ctgctggagg cattgcggcg gctgcccgga gtgacggcgc acccgagcga cgccaacttc
     9841 atactgttca aagtcagaca agcggagcgc gttttcgacg gactgaaaca gcgcggagtg
     9901 ctcatcagga gtctccacgg ttctcatcgc ctgctggccg attgcctgcg ggtgacggtc
     9961 ggcacgccgg acgagaacgc cgctttcctt accgcattga ccgaaaccct cgccgcttga
    10021 tcctccagcc caacttgcgc gcctcgcacc atcagcggtg caagcacggc catgcgtaaa
    10081 gccgaaatca cccgcgacac gaaagaaacg aagatcggcg tcaagctcga cctcgacggc
    10141 agggggaggg cgaagatcgc gaccggcgtg ccgtttctcg accacatgct cgagcaggtc
    10201 gcgcgccacg gggcgtttga cctggaggtc tccgccaagg gcgacctgcg catcgacgcg
    10261 caccacaccg tggaggatat cggcatcacc atcggccagg cattcgccaa ggcggtgggt
    10321 gacaagaaag gcgtgcgccg ctacggccac gcctacgtgc cgctcgacga ggcgttgtcg
    10381 cgggcggtcg tggacctctc gggccggcct ggcctcgagt accacatgga attcacccgc
    10441 gcgcgcatcg gcgaattcga cgtggatctg gtgcacgagt tcttccaggg ctttgtcaac
    10501 cacgcccaag tcacgctcca ccttgacaac ctcaagggca ccaacgccca ccaccaggcg
    10561 gaaaccgcgt tcaaggcgtt cgcgcgcgcg ctgcgcatgg cggtcgaggc cgacccgcgc
    10621 gtgaagggcg tcccttccac gaagggcagc ctgtaacgga gctgtcggca ttcagctatc
    10681 agccgtcagc acacccgccg tcggtgacac gatgtacttc cactccctgc tgcaagctga
    10741 ccgctgaatg ctgattgctg aacgctgttt atgaccgacg tcgctgtcgt ggattacggc
    10801 atgagcaacg tgcgctcggt ggctctcgcg atcaggcgcg tggcgccgca actcaaggtt
    10861 tcggtgacaa gcgacgcgaa cgtggtaaat cgcgctgtgc gcgtggtgtt cccgggccag
    10921 ggcgcgatgc cggactgcat gcgcgagatg gacgcgcggg ggctgcgcgg cgccgtgctg
    10981 gaggctgcgc gcgccaagcc gttcctcggc atctgcatcg ggctgcaaat gctgttcgag
    11041 gcgagcgagg aaggcgatac cccgggtctg gggctgttgc cgggccgggt gcggcgtttc
    11101 ccgcccgaga aaatggtgga cgcggaaggc gcccggctca aagtgccgca catggggtgg
    11161 aacgaagtgc ggcagaccgt cccgcaccct ctgtgggaag gcatcgcgca ggcgagccgc
    11221 ttctatttcg tgcacagcta tttccccgaa cccggcagcc gcgggctcac ggcgggcgag
    11281 agcgtctacg gcgtgacgtt tacctgcgcg gcggcgcgcg acaatgtgtt cgccgtgcaa
    11341 ttccacccgg aaaaaagtga tgcggccggc ctgagactgc ttgccaattt catcggctgg
    11401 caaccagaca cgcaacgcgc cgaacatagc gctacggcat cgccggccgc atagcagcct
    11461 gtcgaacttt gccctgcgac aggctgctaa agcaaaaccg cccaaggact agaagtgaag
    11521 ttgaaccaaa tgatctctac cgaccgaata ttggaagccg cagcgggatc gtgcccggtt
    11581 cccgaggtta gcttttttgc cattccattc tattgccggc ggttttgcct caggagtttg
    11641 tcggggcaaa gcccgacaaa cccctaacgc catgctgatc atccccgcga tcgatctcaa
    11701 ggacggcaaa tgcgtgcgcc tgaaacaggg cgcgatggac aaggctacgg tcttttccga
    11761 cgacccgccc gcgatggccg tgcaatggct caagctcggc gcgcggcggc tgcacgtcat
    11821 tgatctcaac ggcgcggccg ccggccgtcc caagaacgag caggcgatca agtcgatcac
    11881 ggaggcggtg ggggggaagc ttccgatcca gctcggcggc ggcatccgcg atctcgacac
    11941 catcgagaag ctgctcgact ccggagtgag ctacgtcatc atcggcacgg ccgcggtgaa
    12001 gacgcccggt ttcctccacg atgcgtgcac cgcgttcccc gggcacgtgc tggtcgcgct
    12061 cgacgcgaag gacggcaagg tagcggtcga cggctggtcg aagatgaccg gtcacgaggt
    12121 gcttgatctc gcgaagaaat tccaggacta cggcgtggat gcgatcatct ataccgatat
    12181 cgggcgcgac ggcatgatga ccggcgtcaa catcgctgcg acggtggcgc tggcgaaaga
    12241 gctgacggtg ccggtgatcg cgagcggcgg catcaacggt ctcgaggacg tcaaggcgtt
    12301 gtgcaaggtc gagcacgagg gcatcatcgg cgccgtcacc gggcgcgcca tctacgaagg
    12361 caagctggac tttgcgaagg cccagaaatt ggctgacgag ttctccaaga aaaacgaggg
    12421 taatgggaaa tgagtaatgg gtaatgggcg agtcctctcc cgctttttga cccatcaccc
    12481 atcacccatc acccatcacc caaatgggcc tcgccaagcg catcatcccc tgcctcgacg
    12541 tcaccgccgg gcgcgtggtg aaggggatcc acttcgtcgg cctgcgcgat gccggcgacc
    12601 cggtggaggt cgcgcggcgc tacaacgagc agggcgcgga cgagctcgcc ttcctcgaca
    12661 tcacggcgag cagcgacgga cgcgacctga tcgtgagcgt ggtcgaggcg gtggcggagc
    12721 aggttttcat accgttgacg gtagggggcg gcgtgcgttc ggtcgcggac gtgcagcggt
    12781 tgctcaacgc cggcgcggac aaggtgagca tcaataccgc cgcggtgcag aatccccggc
    12841 tggtggagga agcgaccgcg cgttacggct cccaatgcat cgtcgttgca gtggacgcga
    12901 agcgcgtgcc cggtcccggt gcgccgcgct gggaggttta cacccatggc gggcgcaggc
    12961 cgacggggct cgatgcgctg gagtggggcc gcagaatgga ggccgccggc gccggcgaga
    13021 tcctgctcac cagcatggac cgcgacggca cccgcagcgg attcgatctc gagctgaccc
    13081 gcgcgttctc cgatgcgctc tcgatcccgt tgatcgcgag cgggggcgtg ggcagcctcg
    13141 atcacctggc cgagggcgtc ctgcaaggcc gcgccgacgc ggtgctcgcc gcgagcatct
    13201 ttcattacgg cgagttcacc gtgcgccagg ccaaggagca cctggcgcgg cggggcatcg
    13261 aagtccggct ctgaagattc gggactaggg actaggggct agggactagt atgatggttg
    13321 ccatgaatac gcttcgagcc ccgagcctcg agtctcgagc cccgagcccc gcatcatgaa
    13381 cgaaagctgg ctcgacaagg tcaagtgggc gcaggacggt ctggtgccgg cggtggcgca
    13441 ggaaacgggc agcgggcgcg tgctcacggt ggcgtggatg aaccgcgaag cgctcgggaa
    13501 gacggtgcag accggcgagg cgcactactg gtcgcgctcg cgcgggaagc tgtggcgcaa
    13561 gggcgaagag tccggccacg cgcagaaggt caggacgatc cgcctcgact gcgacgagga
    13621 cgtgatcctg ctggaagtgg aacaggtggg cggaatcgcc tgtcatactg gacgtcacag
    13681 ctgcttcttc cggcggctcg acggggccca gtgggtggtg accgatccgg tgctgaagga
    13741 ccccaagggc atttacaagc gatgatcaac gcaaacatac tgcggcggct gggaacagtc
    13801 atcgccgagc gcaaacagag cgatccctcg gcttcctacg tggcgagcct gttcgcgaag
    13861 ggcgaggacg cgatactgaa aaagctctcg gaggaggcgg ccgaaacggt gcttgccgcc
    13921 aagggcggtg ataaactgca tatcgtgcgc gaaaccgccg acctgtggtt ccacagcctg
    13981 gtgctgctgg catggcacgg gcttgcgccc gaggacgtgc tcgccgagct gcagcgccgc
    14041 gagggcattt cagggattga cgagaagaag tcccgcggct gagcggggct tcctgaggag
    14101 agcgatatgg gctcattcag catctggcac tggctgatcg tgctgctggt ggtagtcctg
    14161 atcttcggca ccaagaagct gcgcaacatc ggcgccgatc tcggcagcgc ggtgaagggc
    14221 ttcaaggacg ggatgaagtc cgaggaggac aagtccgcgc aggcgaaggc ggagatcccg
    14281 cccaccaccg gccagaccat cgaaggcgaa gccaagaaag agacgcccaa ggcgtgagga
    14341 gcgaggcgtg aggggtgagg tgcccggacg gcacccgatc ggtttccctc ctcactcctg
    14401 acccctcact cctcacctcg catgttcgac atcgggttct ccgagctgct ggtaatcgcg
    14461 atcgtggcgc tgatcgtgat tggcccccag cgcctgccca cggtcgcgcg cacgctgggc
    14521 catctcttcg gccgcatgca gcgctacgtc aacgacgtca aggccgacat cgcgcgcgag
    14581 atggagctcg aagaactcaa gaagatgcag gcgagcatgg aagatgcggc gcgctcgatg
    14641 cgcgattcgg tcacccggga agtctccgaa accgaaagcg agctgaatcg gctcgcgcag
    14701 gcggcgcagc cggcgcccga tgcttattcc gacgcccccc cgcaggaacc ggcggccaaa
    14761 tccgcttcgc agcagctcga tctggcgctg gatcagtcgg caaatgcgcc gactcagaaa
    14821 caagcctaga gtcggttatg ggtgacaggt aatggatgat gcgcgggcgg cgcagccgac
    14881 gcccgacgcg ttgcggagca gcgattcacc actcaccagt cacgcttcat caggaatgac
    14941 ggaagacacc tttatttccc atctgatcga gttgcgggac cgcctcctgc gctcgctcgt
    15001 cgcaattgcg gcggtgatga ttgtgctcgc gatctggccg ggaatcgggg gcgtctacga
    15061 c
//
LOCUS       DBNGWZ010000301         5058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2301153, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000301 DBNGWZ010000000
VERSION     DBNGWZ010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2301153"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="AC16CX_08860"
     CDS             <1..1434
                     /locus_tag="AC16CX_08860"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABCB family ABC transporter ATP-binding
                     protein/permease"
                     /protein_id="HIR3012658.1"
                     /translation="GVSRDIERGTNGVSTLLSYMLFSIVPVILEFSLVAAVLFARFDW
                     RFVAVTFAAVALYIGYTFAISEWRIGIRRRANELDSRANTRAIDSLLNYETVKYFNNE
                     EYEARRYDENLRSYETAAVKSESSLGLLNVGQSLIIAAAVTVLMILAAEGVAADRLTL
                     GDLVLINGLLIQLYIPLNFLGMVYREIKQALIDMDRMFRLLEENREVEDRPGAAPLPE
                     GGAMIRFDRVSFSYDGKRQILHDVSFDVAPGAKVAVVGASGSGKSTLARLLYRFYDVD
                     GGAILINGVDVQDVTQSSLRAAIGIVPQDTVLFNDTIYYNIHYGRPGATREAVIAAAQ
                     AAHIHDFIESLPDGYEAGVGERGLKLSGGEKQRVAIARAILKNPRILIFDEATSALDS
                     RAEKSIQAELNRIAHGHTTLVIAHRLSTVMDADQILVMDHGRIVERGTHRELLERGGS
                     YAQMWTLQQREEADRARAEKARVQAVG"
     gene            complement(1574..3016)
                     /locus_tag="AC16CX_08865"
     CDS             complement(1574..3016)
                     /locus_tag="AC16CX_08865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3012659.1"
                     /translation="MSFAVKGWRWLPGAIRWNGTRTLRRQLIAWLAGPLLILWSVSTL
                     IDYDIAKRFVNLAYDRALLEAAQDIGRQVKIVNNQIYVDLPEVAQQILQTTESGRLYY
                     LVTGPNQEFVTGEPDLPPPPDEAPGATQDRVRYYDNEYQGSVIRAVALHVPAQAGSGR
                     GAVLIQVAERVTVRDEFARQIILRMALPQGFLILLAGLAVWYGVGRGLAPLSTLRREI
                     QSRSHRDLSALPEEQAPQEVRPLIRAMNDLLQRLAAAISAQQRFIADAAHQLRTPVAG
                     IKTQTELALRQSPSGDAQATLKQLYNATVQMTRLVNQLLSLARAEPGAGRSLGLEPLD
                     LVDLARETTTEWVPLALARNIDLGFDSTLSSARVEGDPFMLREMLNNLLDNAVRYTQP
                     GGQVTVRVTPETRGVTLSVEDNGPGIPEAERERAFERFHRVLGTGADGCGLGLAIVRE
                     IALSHGAATSLASGINNTGTLVRIFFPTAA"
     gene            complement(3062..3742)
                     /locus_tag="AC16CX_08870"
     CDS             complement(3062..3742)
                     /locus_tag="AC16CX_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3012660.1"
                     /translation="MRILIVEDDPVLGDGLIRSLRAVDYAVDCVNDGAEADHVLAAQN
                     YDLVILDLGLPKLDGFEVLRRLRRRGSRMPVLVLTARDALEDRVKGLDLGADDYLAKP
                     FDLPELEARVRALIRRGQSGGGSLIAHGALTLDTAGRRATLNGEPLELSARELGVLEV
                     LMLRSGRVVNKEQLAGQLYGWDEEVGANAIEVYVHRLRRKLEPAGVTIRTIRGLGYLL
                     EKPGHVRN"
     gene            4176..5036
                     /gene="pbpG"
                     /locus_tag="AC16CX_08875"
     CDS             4176..5036
                     /gene="pbpG"
                     /locus_tag="AC16CX_08875"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR3012661.1"
                     /translation="MKPQLLALGFALALAASAAARAQAHDPAVVAKAGVLSLKSASAL
                     VVDQSGQRVLYAKNVETVVPIASITKLMTAMVVLDAGLPLEERIAISRADSDGIKRTR
                     SRLKVGTVLTRGDLLKLALMASENRAATALARAYPGGTRAFVAAMNHKAVELGMWRTR
                     FVDGSGLSSENVSTAQDLARLVGAAYRYPLIREYTTDSTHTVELANGRRMHFSNSNRL
                     VNHAGWNIGLSKTGYISEAGRCLVLQARIAATPVIIVLLDSWGRLTRIGDANRIKKWM
                     ESNFLRQPAG"
BASE COUNT          853 a         1652 c         1693 g          860 t
ORIGIN      
        1 ggcgtctcgc gcgacatcga acgcggcacc aacggcgtgt ccacgctgct ctcatacatg
       61 ctgttctcga tcgtgccggt gatcctggag ttcagcctgg tggcggcggt gctgttcgcg
      121 cggttcgact ggcgcttcgt ggcggtgaca tttgccgcgg tcgcgctcta tatcggctac
      181 accttcgcca tctcggagtg gcgcatcggc atccggcggc gcgccaacga gctcgattcg
      241 cgcgccaaca cgcgcgccat cgacagcctg ctcaactacg agacggtcaa gtatttcaac
      301 aacgaggagt acgaggcgcg ccgctacgac gagaacttga ggagctatga gacggcggcg
      361 gtcaagagcg agtcttcgct ggggctgctc aacgtcgggc aaagcctgat catcgccgcc
      421 gcggtgacgg tgctgatgat cctggcggcg gaaggcgttg cggccgacag gctcacgctg
      481 ggcgatctgg tgctgatcaa cgggctgctc atccagctct acatcccgct caacttcctc
      541 ggcatggtgt accgcgagat caagcaggcg ttgatcgaca tggaccgcat gttccggctg
      601 ctggaggaga accgcgaggt cgaggaccgc ccgggcgcgg cgccgctgcc ggaaggcggc
      661 gcgatgatcc gtttcgaccg cgtcagcttc agctacgacg ggaaacggca gatcctgcac
      721 gacgtgtcgt tcgacgtcgc gccgggcgcc aaggtggcgg tcgtgggcgc aagcggctcg
      781 ggcaagtcca cgctcgcgcg gctgctctat cgcttctacg acgtcgacgg gggcgcgata
      841 ttgattaacg gcgtggacgt ccaggacgtg acgcaatcga gcctgcgcgc ggcgatcggc
      901 atcgttccgc aggatacggt gctgttcaac gacacgattt attacaacat tcattacggc
      961 cggcccgggg cgacgcgcga ggcggttatc gccgcggcgc aggcggcgca catccacgat
     1021 ttcatcgaga gcctgccgga cggctacgag gcgggggtgg gcgagcgggg cctcaagctc
     1081 tcgggcggcg agaagcagcg cgtcgccatc gcccgcgcca tcctcaagaa cccgcgcatc
     1141 ctgatcttcg acgaagcgac ctccgcgctc gactccaggg cggaaaagtc gatacaggcc
     1201 gaattgaacc gcatcgcgca cgggcatacc accctcgtca tcgcccaccg gctttccacc
     1261 gtgatggatg cggaccagat cctggtcatg gaccacggcc gcatcgtcga gcgcggcacc
     1321 caccgcgagc tgctcgagcg cggtgggagc tacgcccaga tgtggaccct gcagcagcgg
     1381 gaagaggccg atcgtgcgcg cgctgagaaa gcgcgcgtcc aggcagtggg ataagtcgcg
     1441 cgcgcgaaaa ggcgtgagcc gtgaatggtg aatagtgagt agtgggtaga atttagaccg
     1501 ggactgtgtc ccaaccaggg gcgtcgtcag gtttggtctg ctcaccgatc actactcacc
     1561 actcacggcc ttttcaggcc gctgtcggga agaagatgcg caccagcgtg ccggtattgt
     1621 ttatcccgct cgccaagctc gtcgccgctc cgtgcgagag cgcgatctcc cgcacgatcg
     1681 cgagacccag gccgcagccg tcggcgccgg tgccgagcac gcggtgaaag cgctcgaacg
     1741 cccgctcccg ctccgcttcc ggtatgcccg gcccgttgtc ctcgacgctg agcgtgaccc
     1801 cgcgcgtctc cggcgtgaca cgcaccgtca cttgcccgcc gggctgcgta tagcgcactg
     1861 cgttatccag caggttgttg agcatttccc ggagcatgaa cgggtcgcct tcgacgcgcg
     1921 cgctggaaag cgtgctgtcg aagccgagat cgatgttccg tgccagcgcg agcggaaccc
     1981 attcggtggt ggtttcgcgg gcgagatcga ccagatcgag cggctccaga cccaggctgc
     2041 gaccggcgcc tggctccgcg cgggcgagcg agagcagctg gttgacaagg cgcgtcattt
     2101 gcacggtcgc gttgtacagc tgcttcaatg tcgcctgtgc gtcgcccgac ggcgattgcc
     2161 gcagcgcgag ttcggtctgg gtcttgattc ccgcgaccgg cgtgcgcaac tggtgggcgg
     2221 cgtccgcgat gaaccgctgc tgggccgaga tcgcagcggc gaggcgctgc aacaggtcgt
     2281 tcatcgcgcg aatcaatggt cgcacttcct gcggcgcctg ttcctcgggc agggccgaga
     2341 ggtcgcggtg cgagcggctt tggatttccc ggcgcagcgt ggaaagcggg gcaagacccc
     2401 ggccaacgcc gtaccagacc gcaagtccgg cgagcaggat caggaagccc tgcggaagcg
     2461 ccatgcgcag gatgatctgg cgcgcaaatt catcgcgcac cgtgacgcgc tcggcgacct
     2521 ggatcagcac cgcgccccgg cccgagccgg cctgggccgg cacgtgcagc gccacggcgc
     2581 ggatcacgct accttgatac tcgttgtcgt aataccgcac ccggtcctgc gtcgctccgg
     2641 gcgcctcgtc cggcggtggc ggcagatccg gctcgccggt gacgaattcc tgattgggac
     2701 cggtgacgag gtagtagagg cgtccggact ccgtggtctg caggatttgc tgggccactt
     2761 ccggcaggtc cacgtaaatc tggttgttga cgatcttgac ttggcggccg atgtcctgcg
     2821 cggcctcaag cagcgcacgg tcgtaggcga ggttgacgaa tcgcttggcg atatcgtagt
     2881 cgatgagcgt actgaccgac cacagaatca acaacggccc ggccagccag gcgatgagct
     2941 ggcgccgcag cgtccgggtg ccgttccagc gtattgcgcc cggaagccag cgccacccct
     3001 tcaccgcgaa actcatgttc ccggtctacc tgcggtaaca gacgtcgcga gctggaacgg
     3061 gctagttgcg gacatgcccg ggtttttcga gcagatagcc aagaccgcgg atggtgcgga
     3121 tggtgacgcc agcgggctcc agtttcctcc gcagccggtg cacgtaaacc tcgatcgcgt
     3181 tggcgccgac ttcctcgtcc cagccgtaga gctgtccggc cagttgctcc ttgttcacca
     3241 cccggccgct gcgcagcatc agcacttcca ggaccccgag ctcgcgcgcc gaaagctcga
     3301 gcggctcgcc gttgagcgtc gcgcggcggc cggcggtgtc gagcgtgagc gcaccgtgcg
     3361 cgattagcga tccgccgccg gactggccgc gccggatgag cgcccgcacg cgcgcctcga
     3421 gctcgggcag gtcgaacggc ttggcgagat aatcgtcggc gccgagatcg agccccttga
     3481 cgcggtcttc cagggcgtcg cgggcggtga gcaccagcac cggcatgcgc gagccgcgcc
     3541 ggcgcagccg gcgcagcact tcgaagccgt ccagcttggg cagccccaga tcgaggatca
     3601 ccaggtcgta gttctgggcc gccagcacgt ggtcggcttc ggccccgtca ttcacgcagt
     3661 ccaccgcata gtcgacggcg cgcagcgagc ggatcaaccc gtcacccagc accggatcgt
     3721 cttcgacaat gaggatacgc atcggatttg gctggacccg cggtccggca agacggagga
     3781 tagtctatcc cagcgcgctc gcgcgcgacc cgccggatgt aaggtttgcg taaggcagag
     3841 gggatagcct ttatccactc acgcgggcgg ctcagccgaa acgaccgcgc aggtaagcag
     3901 gttgcttagt ctttcctcct cggaggccta gcagtagcag ttatctgtgc tgagcgggta
     3961 attgcatggc ctctttgcct ccgccgggac aacccacact gtcccgagcg gagttttttt
     4021 ttgccccgag ccgacgccgc agccattcgg gcgcgatccc cgcgctgtta aatagttgct
     4081 tgaaaaaatc gagctaaccc tgtattttag cgcctgattc aggtggtggc gttgccgcgt
     4141 acggcgccgc ggtacctggc agggaggaaa ggggtttgaa accacagctg cttgctctcg
     4201 gcttcgctct ggcgctggca gcgtccgccg cggcgcgcgc gcaggctcac gatccggctg
     4261 tggttgccaa ggccggcgtc ctcagcctca agtccgcttc cgccctggtg gtggaccaga
     4321 gcgggcagcg ggtgctttat gccaagaatg tcgagacggt cgttccgatc gcctccatca
     4381 ccaagctgat gaccgcgatg gtggtgctgg acgcgggcct gccgctcgag gagcgcatcg
     4441 ccatcagcag ggcggactcg gacggaatca agcgcacccg ttcccgcctg aaagtgggca
     4501 ccgtgctgac gcgcggcgac ttgctcaagc tcgcgctcat ggcttccgaa aaccgcgccg
     4561 ccacggcgct cgcccgcgct tacccgggcg gcacgcgcgc cttcgtggcg gcgatgaacc
     4621 acaaagcagt cgagctcggc atgtggcgca cccgcttcgt ggacggcagc gggctgtcga
     4681 gtgaaaacgt ctcgaccgcg caagacctcg cgcggctggt gggcgccgcc taccgctacc
     4741 cgctgatacg cgagtacacc acggattcca cgcacaccgt cgagctcgcc aacggcaggc
     4801 ggatgcactt ctcgaattcc aaccggctgg tcaaccacgc ggggtggaat atcggcctgt
     4861 cgaagaccgg ctatatcagc gaggccgggc gctgtctcgt gctgcaggcg cggatcgcgg
     4921 cgacccccgt gatcatcgtg ctgctcgact cgtggggcag gctcacgcgc atcggcgacg
     4981 ccaaccgcat caagaagtgg atggagagta acttcctacg tcagccggcg ggctgaccgc
     5041 ccggccgcgg cctcgcgg
//
LOCUS       DBNGWZ010000302         1901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3056308, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000302 DBNGWZ010000000
VERSION     DBNGWZ010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3056308"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16CX_08880"
     CDS             <1..264
                     /locus_tag="AC16CX_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012662.1"
                     /translation="RTKAEVLDNLVQAALRVQARYPGQAHFVLVLRSDGEPALEERKR
                     AFRAKAVALGIPVFDEMANAGRALAALAFHERFVHSRKSVNRE"
     gene            353..1555
                     /locus_tag="AC16CX_08885"
     CDS             353..1555
                     /locus_tag="AC16CX_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3012663.1"
                     /translation="MYTYDFRVTGYPFRLHSGNNALDKLPEEVARQQATRAFIVCGRT
                     VSRKTPLISRMKHLLGERLAGVFDEIDKDTSRSSVMRAVEAARAARADLVIGVGAGSV
                     IQGARIVTILLAEKRPVEDLITQYPEHGPAISPKLMEPKLPIINVLTAPTTAQNRAGS
                     RMKDDATARGMEFFDPKTRPVAIFWDTDALLTAPVSLIRTGAATIWCRAVMNLGATDD
                     NPLVEGDRQQSYRLAAGALARIADATDPTPRFDLCAATLLQNREADDGGAQFARHWAM
                     RVAYAFSSALISVFPQVGQGEGYSALVGTVLRRLGGRDPEAMARIAAGLGMDGIAPAE
                     APRRIADRMDEVFRSLGMPTRLSVLGIERGRLTQVLELSLRNFNADPKREFVRERGTL
                     EEVLNAAW"
     gene            1715..>1901
                     /locus_tag="AC16CX_08890"
     CDS             1715..>1901
                     /locus_tag="AC16CX_08890"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase, FlK family"
                     /protein_id="HIR3012664.1"
                     /translation="MDTLKVGMKHVQEMTVEEKHTTQRGGYRVFSTPSMTQFVEMTAQ
                     QLAAPHLKPGQGQVGTLV"
BASE COUNT          353 a          628 c          622 g          298 t
ORIGIN      
        1 cgcaccaagg ccgaggtgct cgacaacctg gtgcaggcgg cgctgcgcgt gcaggcgcgc
       61 tatccgggcc aggcgcattt cgtgctggtg ctgcgctccg acggcgagcc ggcgctggag
      121 gagcgcaagc gcgcgttccg cgcgaaggcg gtcgcgctcg gcattccggt cttcgatgag
      181 atggcgaacg ccggccgcgc gctggcggcg cttgccttcc acgagcgctt tgtccatagc
      241 aggaaaagcg tgaatcgtga atagtgaatg gtgaatggac cgagtccgca cggcgcgaaa
      301 cgcaaaaaca atccaggtcc actcactatt caccaatcac caatcacgcc tcatgtatac
      361 atacgacttc cgagtgaccg gctacccctt ccggctccat agcgggaaca acgcgctcga
      421 taagctgccg gaggaggtcg cgcggcagca ggcgacccgg gcgttcatcg tgtgcgggcg
      481 cacggtgtcg cgcaagacgc cgctcatctc ccgcatgaag catctcctag gcgaacggct
      541 cgcaggcgtg ttcgacgaga tcgacaagga cacctcgcgc tcctcggtca tgcgcgcggt
      601 ggaagccgcc cgcgccgcgc gcgcggacct cgtgatcggc gtcggcgcgg gcagcgtgat
      661 ccagggcgcg cgcatcgtga cgatcctgct cgccgagaag cgcccggtcg aggatctcat
      721 cacccagtat cccgagcacg gccctgcgat cagccccaag ctcatggagc cgaagctccc
      781 gatcatcaac gtgctgactg cacccaccac ggcccagaac cgcgcgggct cgcgcatgaa
      841 ggacgatgcg accgcgcgcg gcatggagtt cttcgatccg aagacgcgcc ccgtcgcgat
      901 cttttgggac accgacgcgc tgctcaccgc gccggtcagc ctcatccgta ccggcgccgc
      961 gacgatctgg tgccgcgcgg tgatgaatct cggggccacc gacgacaatc ctctggtgga
     1021 aggcgaccgg cagcaatcgt atcggcttgc cgcgggggcg ctcgcgcgca tcgccgacgc
     1081 cactgatccg acgccgcggt tcgacctgtg cgcggcgacg ctgttgcaga accgcgaagc
     1141 ggacgacggg ggcgcgcagt tcgcgcgcca ctgggcaatg cgcgtcgcct acgccttctc
     1201 ctcagcgctg atcagtgtgt tcccgcaggt cggccagggc gagggctaca gtgcattggt
     1261 gggcaccgtg ctgcgccgcc tcggcggccg cgaccccgag gcgatggcgc ggattgcagc
     1321 cggtctcggt atggacggca ttgcgcccgc cgaggcaccg cgccgcatcg cggacagaat
     1381 ggacgaagtg ttccgctcac tcggaatgcc cacgcggctt tccgtgctcg ggatcgagcg
     1441 cgggcgcctg acgcaggtgc tcgaactctc gctcaggaac ttcaacgccg atcccaagcg
     1501 cgaattcgtg cgcgagcgag ggacgctgga agaggtgcta aacgccgcgt ggtaagtacg
     1561 gaccgatcca cggcctatgt gccacagcgg ctagcgtccc tgaccaaaag ttctccgaag
     1621 taaaaaatcg gacgcctctt tcggcggccg attttttgga cccgctatgc gtcgactaca
     1681 ataaacagtg atttaacctc atgcatggag aagaatggac acgctaaaag tcgggatgaa
     1741 gcacgtccag gaaatgacgg tcgaggagaa gcacaccacc cagcgcggcg gctaccgggt
     1801 gttttccacg ccgtcgatga cgcagttcgt ggagatgacg gcgcagcagc ttgccgcgcc
     1861 tcacctgaag cccggccagg ggcaagtcgg gaccctcgtc a
//
LOCUS       DBNGWZ010000303         5464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2301435, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000303 DBNGWZ010000000
VERSION     DBNGWZ010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2301435"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1732
                     /locus_tag="AC16CX_08895"
     CDS             245..1732
                     /locus_tag="AC16CX_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3012665.1"
                     /translation="MEIKGFACVQKTAGLVLGVAAAVLLAACQTTATQEARDPQQAEA
                     RNMRLVGYNDLQARTAYQPVIHRNGNRWIAYVGHHGGAVLNPLSGNKENNGTSLVDVT
                     DPKQPKYLAHIPGDEGAGEAGAAQMVRVCNGRDLPRADRAKVYMLRTFGNKGHEIWDV
                     TVPERPALLTTIVKGLKGTHKNWWECDTGIAYLVSGVPDWRSTRMMQVYDLSDPAKPV
                     LIRNFGVPGQEPGSTGPQSRFSMHGAISTGPKGNRIYVGYGTVSNGLIQIIDREKLLK
                     GPREPTPANLRYPVIAQVETPSYMGAHTTLPIPGLEIADLAKYWGPNAGPKHRDIIVV
                     VNESTSNECREARQQAYFIDITQETRPFAISNFDVPEATHNFCTRGGRFGAHSSNENQ
                     PPMYAKRIVFMTWFNAGVRAVDIRDPYRPREIGYYIPAVTDRTAERCVERDGKKSCKI
                     AIQTNNVEVDDRGYIYIVDRANTGMHILEPTGELRRISNFDAAVK"
     gene            complement(1754..3673)
                     /locus_tag="AC16CX_08900"
     CDS             complement(1754..3673)
                     /locus_tag="AC16CX_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3012666.1"
                     /translation="MVFWSLDKVEAVADKNKHLRFKDVIVIGAGVAGLSAARVLADRN
                     VNVRVLEARDRIGGRVWTVDRLDLGAQWIHGTEGNPVTGLCRRLGIPLLYVGGDSSYL
                     GGWENLHLLDHRGKAVSSASKARSIAVADAIRDEFERWRSAMHPGKAGDVPAAAAFSR
                     IVRRHRLETSKAREADWHVRICTRDDWGGFRDTLSALYWEEGYQVYGYGDSAFSDGYG
                     VVTGRLAQGLPIRLNTAVTRVKHGAKGVTVLTGDGEFHADAAIVTVPLGVLKSGVLKF
                     EPALPRTKRDAIQRLGVGCLAKLALFYDEPFWPREPYVFGVVDGANTMSPTLAVNMWV
                     THRLPCLVFIVGGELGQALERWPVDKAVAWAQAALRRMFGQAPDPVRTLRTGWTLDPF
                     ALGAYSHVPVGATPADFDRLAEPVGKRLLFAGEATCRQHWACVHGAHVSGLREAARLL
                     DDPSVLPPLRITEDRRWRDMMVRAARFFQMRATSADRAETSRRTAVLRKSEVFAEVEE
                     RELELLATMFERRTLEPGETLCRAGDEAREIYVVVSGRIGVRQGSGPAHGKRPRAALA
                     KAGPGTVIGEYGLVTPDARRTATLYAIGRAEVLSLDYPRFRQFVLAFPQCALAMLGVT
                     VQRLQREMALARSKP"
     gene            complement(4123..4776)
                     /locus_tag="AC16CX_08905"
     CDS             complement(4123..4776)
                     /locus_tag="AC16CX_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012667.1"
                     /translation="MPPELAESPLFLAAVALAALGAILVLLGIAALLRLRPLRFVIRT
                     LLGLLLLALGMLAGTVALGIQGYRALTREDVAARIVVKPAGPQRFTATFRFPDGREAT
                     YELTGDEIYVDAHILKWHPRANVVGLHTAYELDRVAGRYHAIQEERSAARTVHSLGRD
                     RPVDLFALRRRYTFLAPLLDAEYGSATFIPVAQPAELELRVSTTGLLIREAKPPQKP"
     gene            complement(4783..4935)
                     /locus_tag="AC16CX_08910"
     CDS             complement(4783..4935)
                     /locus_tag="AC16CX_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012668.1"
                     /translation="MIRKLKSGRYRLYSRKKNPRTGRRRNLGTFRTRAAAERHERAIQ
                     YFKRHG"
     gene            4981..5133
                     /locus_tag="AC16CX_08915"
     CDS             4981..5133
                     /locus_tag="AC16CX_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012669.1"
                     /translation="MNTNQRRQIEYSLLFLPFYIGVDQRLSKAKLRFRSVRQVQAGFQ
                     ERKVDR"
     gene            complement(5249..>5464)
                     /locus_tag="AC16CX_08920"
     CDS             complement(5249..>5464)
                     /locus_tag="AC16CX_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3012670.1"
                     /translation="RLGFSFEGIFRQAVIYKGRSRDTAWYAAIDSEWPELERAFVRWL
                     DPRNFDERSNQRLRLSDLTAPILKQRG"
BASE COUNT         1000 a         1790 c         1798 g          876 t
ORIGIN      
        1 gggcagatgg cggtgccgct ggtcgcatct gcaatttcga acgccgtgtt ccagctcacc
       61 ggcgctcggc tgcgccagca gccgatgctg ccggagcggg tgaagctcgc gctggaacag
      121 ggggagcagc gattcgcgta gcagtttgaa aacggcgggg acgtcgcgcc ccgccgcccc
      181 ccggacaatg cgccggccgg gggcggtagt tctgtttgtt ccgtcacgga aggggagctc
      241 caacatggaa ataaaaggtt ttgcctgcgt acaaaagacg gcggggctgg tgctcggggt
      301 ggcggccgcc gtcttgctcg ccgcgtgtca gaccacggcg acgcaggagg cgcgtgatcc
      361 gcaacaggcc gaggcgcgca acatgcggct cgtcggctac aacgacctgc aggcgcgcac
      421 cgcctatcag ccggtgatcc accggaacgg caaccgctgg atcgcctatg tcgggcacca
      481 cggcggcgcg gtcctgaacc cgctcagcgg gaacaaggaa aacaacggca catcgctcgt
      541 ggacgttacg gatccgaagc agccgaaata tctcgctcac attcccggcg acgagggcgc
      601 aggcgaggcg ggcgccgcgc agatggtgcg cgtgtgcaac ggccgcgacc tgccgagggc
      661 ggaccgcgcc aaggtctaca tgctgcgcac cttcggcaac aaaggtcacg agatctggga
      721 cgtcacggtg ccggagagac cggcgctcct gaccaccatc gttaagggcc tgaaaggcac
      781 gcacaagaac tggtgggagt gcgacaccgg tatcgcctac ctcgtctcag gcgttccgga
      841 ttggcgttcg acccgcatga tgcaggtcta cgacctgagc gacccggcaa agcccgtatt
      901 gatccgcaat ttcggcgtgc caggacagga gcccggatcg accggaccgc agtcgcggtt
      961 ctcgatgcac ggcgcgatct ccaccgggcc gaagggcaac cgcatctacg ttggctacgg
     1021 cacggtgagc aacggcctga tccagatcat cgaccgcgag aagctgctga aggggcccag
     1081 ggagccgacg ccggccaacc tgcgttaccc ggtgatcgcc caggtcgaga cgccgagcta
     1141 catgggggcc catacgacgc tgccgatacc cggcctggag atcgccgatc tcgcgaagta
     1201 ctgggggccg aacgcgggcc cgaagcaccg cgacatcatc gtcgtggtga acgaatccac
     1261 ctcgaacgag tgccgcgagg cgcggcagca ggcgtatttc atcgacatca cgcaggagac
     1321 acggccgttc gcgatctcga atttcgacgt gccggaggcg acgcacaact tctgcacgcg
     1381 cggcggacgg ttcggggcgc attcctcgaa cgagaaccag ccgccgatgt acgcgaagcg
     1441 catcgtgttc atgacgtggt tcaacgcggg agtgcgcgcg gtcgacatcc gcgacccgta
     1501 ccggccgcgc gagatcggct actacatccc ggcggttacg gacaggacgg cggagcgctg
     1561 cgtcgagcgc gacgggaaaa agagctgcaa gatcgcgatc cagaccaaca acgtcgaagt
     1621 ggacgaccgc ggctacatct acatcgtgga tcgcgccaac accggaatgc acatcctcga
     1681 gccgacggga gaattgcgca ggatttccaa cttcgatgcg gccgtgaagt gacggcggaa
     1741 cgcggcgtcg cagctacggc ttgctccttg caagcgccat ttcgcgctgc agccgctgga
     1801 cggtcacgcc gagcatggcc agcgcgcatt ggggaaaagc cagcacgaat tgccggaacc
     1861 gggggtaatc gaggctcagc acctccgccc gccctatagc gtacagcgtg gccgtgcggc
     1921 gcgcgtcggg tgtgaccagc ccgtattccc cgatcaccgt tccagggccc gcctttgcca
     1981 atgccgcgcg cgggcgcttt ccgtgcgccg ggccgctccc ctggcgcacg ccgatcctgc
     2041 ccgaaaccac gacgtaaatc tcccgcgctt cgtcgccggc cctgcacagc gtttcgcccg
     2101 gctcgagagt gcggcgctcg aacatcgtcg caagcagttc cagctcccgc tcctcgacct
     2161 ccgcgaacac ctcgcttttc ctcaataccg ccgtccgccg ggaggtttct gcgcggtcgg
     2221 cggacgtggc gcgcatctgg aagaagcgcg cagcccgcac catcatgtcg cgccagcggc
     2281 ggtcttccgt gatgcgcagc ggcggcagca cggacgggtc gtccagcagc cgcgccgcct
     2341 cgcgcagtcc ggaaacgtgc gcgccgtgga cgcaggccca gtgctgcctg cacgtcgcct
     2401 cgcccgcgaa cagcagccgt ttcccgaccg gttccgccag ccggtcgaaa tcagccggcg
     2461 ttgccccgac cgggacgtgc gagtatgcgc ccagcgcgaa tggatcgagg gtccaccccg
     2521 tgcgcagcgt cctgaccggg tcaggcgcct gcccgaacat ccgccgcaat gccgcctgcg
     2581 cccacgcgac ggccttgtcg accggccagc gctctagcgc ttggcccagc tcgccgccga
     2641 ctataaacac caggcaaggc aggcgatggg tgacccacat gttcaccgcc agcgtcgggg
     2701 acatcgtgtt tgcgccgtcc acgacgccga acacataggg ctcgcgcggc cagaacggct
     2761 cgtcgtagaa cagggcgagc ttggcgaggc accccacgcc gaggcgctgg atggcgtcgc
     2821 gcttggtccg ggggagtgcc ggttcgaatt tcagcacgcc cgacttgagc acgcccagcg
     2881 gcaccgtgac gatggcggcg tcggcgtgga actcgccgtc cccggtcagc acggtcacgc
     2941 ctttcgcgcc atgtttcacc cgggtgacgg cggtgttcaa tcgtatcggc aggccctgcg
     3001 ccagccgccc ggtcaccaca ccgtatccgt cggagaacgc gctgtccccg tagccgtaaa
     3061 cctggtatcc ctcttcccag tacaaggcgg aaagcgtgtc gcggaaaccg ccccaatcgt
     3121 cccgggtgca gatgcgcacg tgccaatcgg cctcgcgcgc cttggacgtt tccaggcggt
     3181 gccggcgcac gatgcgagaa aacgcagccg ccgccggaac gtcgccggcc ttgccgggat
     3241 gcattgcgct gcgccagcgc tcgaattcat cgcggatcgc atcggcgacg gcaatgctgc
     3301 gcgccttgct ggccgaagaa accgccttgc cacggtgatc cagcagatgc aggttttccc
     3361 agccgccgag ataggaggaa tcgccgccca cgtagaggag gggtatgccc agccggcggc
     3421 acaggccggt gaccgggttg ccctcggtgc cgtggatcca ttgggcgccg agatcaagcc
     3481 ggtccacggt ccagacgcgg ccgccgatcc ggtcgcgcgc ttcaaggacc cgcacgttga
     3541 cgttgcggtc agcgagcacg cgcgcggcgg acagtccagc gacacccgcg ccgatgacga
     3601 tgacatcctt gaaccggagg tgcttgttct tgtcggccac ggcttcaacc ttgtccaggc
     3661 tccagaatac caagaatttt tggtgcttgg cgcacccgca ttggcttggg gtcccgggcg
     3721 gcagccaagc gatttacatt ggcttgaaga aagcgccaat gtcggcggcg gcgtgttcag
     3781 gcccggccca gggcgggtag cccggatcga cctcccgccg tgatgcgtga agctgccaag
     3841 ctttaaatga caagcgacag ttggcaagtg ataagggaca tggcgccagc gcgcaggcgc
     3901 ggcgcgggaa gtcgccaact tcaaatagcg cgccgagggg cggaagaggg gccccgaatt
     3961 tgttcgggga tctgaccccc ggtggcgcgc aggcggccga ctcgatgccg tgtgatgctt
     4021 gccggttacc aatccgaggc cgcggagtga tgagtgatga gtaagcgggg tcttcaggcc
     4081 ctgttgtttt gtctactcga tccacgaccc acgatccacg aatcacggtt tttgtggcgg
     4141 cttcgcctcc cgaatgagca gcccggtggt tgagacgcgc agctcgagct cggcgggctg
     4201 cgccaccgga atgaacgtcg ccgagccgta ctccgcgtcg agcagcggcg caagaaaggt
     4261 gtagcgccgg cgcagcgcga acaggtccac gggcctgtcc cggccgagcg aatgcaccgt
     4321 ccgcgctgcg gagcgctctt cctggatggc atggtaacgg cccgccacgc ggtcgagctc
     4381 gtaagcggtg tgcagcccca ccacgttggc gcgcggatgc cacttgagga tgtgcgcgtc
     4441 cacgtagatc tcgtccccgg taagctcgta cgtcgcctcg cgcccgtcgg ggaagcggaa
     4501 cgttgcggtg aaacgctgcg ggcccgcggg cttcaccacg atccgcgccg ccacgtcctc
     4561 gcgcgtcagc gcgcggtagc cctggatgcc gagcgcgacc gtgcccgcga gcatcccgag
     4621 cgcgagcagc agcagcccga ggagcgtccg gatgacgaac cgcaacggcc tcaggcgcag
     4681 cagcgccgcg atcccgagga gcaccaggat cgcgccgagc gcggcgagtg cgacggcggc
     4741 gaggaagagc ggcgattctg cgagctcggg cggcatggtc ggtcatccgt ggcgcttgaa
     4801 gtactgaatg gcgcgctcgt ggcgctcggc ggccgcgcgc gtcctgaacg tgccgaggtt
     4861 gcgccgcctg ccggtccgcg ggtttttctt gcgggagtaa aggcggtacc gccccgactt
     4921 gagcttgcgg atcatttgaa gcgcgtctca aaaacgccta aagaccttga ggaaccgccg
     4981 atgaacacca atcaacgcag acagattgaa tacagcctgc tgtttcttcc attttacatc
     5041 ggcgttgacc agcgattatc caaggctaaa ttacgtttca gatctgtgag gcaagttcaa
     5101 gccggatttc aagaaagaaa ggttgaccgt tgatcaatcc agataaacgc ggccataaat
     5161 ccttgctgat ggcttttctg gaattcgatt atcggtgttc atccgcgttc atctgcggtt
     5221 tcattcagtt tttcaggaca gcttctagtc agccgcgctg cttgagtatc ggagccgtca
     5281 aatcggaaag acgcaagcgc tggttgctgc gctcgtcgaa attccgggga tcgagccagc
     5341 gcacgaaagc ccgttcgagt tcgggccact cgctgtcgat cgccgcatac cacgccgtat
     5401 cccggctgcg gcccttgtag atgaccgcct ggcggaagat gccctcgaag gaaaatccaa
     5461 gccg
//
LOCUS       DBNGWZ010000304         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2804575, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000304 DBNGWZ010000000
VERSION     DBNGWZ010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2804575"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16CX_08925"
     CDS             complement(<1..470)
                     /locus_tag="AC16CX_08925"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3012671.1"
                     /translation="MPWDARGVPPALRRWLERRKAPMRVLIPGCGSGYEVRAFAERGH
                     DVLAIDFSDAALEAARRGLGELAARVRKADFFALQEPPFDLVYERAFLCALPRQLWQD
                     WGRRVAELVRPGGELAGFFYFDDNQRGPPFGISVQRLNELLEDGFELTENLPVA"
     gene            complement(542..2062)
                     /gene="ubiB"
                     /locus_tag="AC16CX_08930"
     CDS             complement(542..2062)
                     /gene="ubiB"
                     /locus_tag="AC16CX_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HIR3012672.1"
                     /translation="MHLFRLLKILSVSVRYGLDEFFLGHERVRGLRALFGAAFFWRTL
                     AEPRAVRLRQALEALGPIFVKFGQVLSTRRDLLPPDIADELAKLQDRVPPFPAEQVMA
                     ILHRVYGRPAGQVFRDLSSLPVASASVAQVHLARLPDGTEVAVKVLRPGSTEVIAGDL
                     ALLHTGATLIEWLWSDGRRLRPHEVVDEFAKHLEVELDLLREASNASQLKRNFEGSPL
                     LLVPQVHWDYCSSEVMVMQRMHGTPVSQVAALRAQGVDIPRLARAGVEIFFTQVFRDG
                     FFHADMHPGNILVTPDGRYVALDFGIMGTLTEIDKNYLAQNFLAFFRRDYRRVAEAHI
                     ESGWAPKDTRVDDFEAAIRAVCEPVFAKPLKGISFGRVLLRLFQTSRRFNIQVQPQLV
                     LLQKTLLNIEGLGRELDPELDLWATAKPYLERWMNEQVGWRALLRTLKNEAPYWAATL
                     PQLPRLAHRLLAEDRIGELKRSVDRLADENARRNDLLVVLLGVLVAALVVAVLGLL"
BASE COUNT          356 a          736 c          720 g          296 t
ORIGIN      
        1 tccgcgacgg gcaggttttc cgtcagttca aaaccatctt ccagcagctc gttcaaccgc
       61 tgcacgctga tgccgaacgg cgggccgcgc tggttgtcgt cgaagtagaa gaagcccgcg
      121 agctcgcccc ccggacgcac cagctccgcg acccggcggc cccagtcctg ccagagctgc
      181 cgcggcagcg cgcacagaaa ggcgcgctcg tagacgaggt cgaacggcgg ctcctgcagc
      241 gcgaagaaat ccgccttcct gacgcgcgcc gcaagctcgc cgagcccgcg cctcgccgcc
      301 tcgagcgccg cgtcggagaa atcgatcgcc agcacgtcgt gcccgcgctc ggcgaaagcg
      361 cgcacctcgt agccgctgcc gcagccgggg atgagcacgc gcatcggcgc cttgcgcctc
      421 tcgagccagc gccgcagcgc cggcggcacg ccgcgcgcgt cccaaggcat gacgcggccg
      481 cgaaaccgcg tgtcccagaa gtccgctcgc gagctgtcct gtgccagcgt atcccctgcc
      541 gtcagaggag gccgaggacc gcgacgacga gggcggcgac gagcacgccg agcaacacca
      601 ccagcagatc gttacggcgt gcattctcgt cggccagccg gtcgacggat cgcttcagct
      661 cgccgatgcg gtcctcggcg agcaggcggt gcgcgaggcg cggcagctgc ggcagcgtcg
      721 ccgcccagta cggcgcctcg ttcttcagcg tgcgcaggag cgcgcgccag ccgacctgct
      781 cgttcatcca gcgctcgagg tagggcttcg ccgtcgccca gaggtcgagc tcggggtcga
      841 gctcgcgccc caggccttcg atgttgagca gcgtcttctg cagcagcacg agctgcggct
      901 gcacctggat gttgaagcgg cgcgaggtct ggaacagccg cagcagcacg cgcccgaagg
      961 agattccctt gaggggtttc gcgaaaaccg gctcgcatac cgcgcggatc gctgcctcga
     1021 agtcgtccac gcgcgtgtcc ttaggcgccc acccggactc gatgtgcgct tccgccacgc
     1081 gccggtagtc gcggcggaag aaggcgagga agttctgggc gaggtagttc ttgtcgatct
     1141 cggtcagcgt tcccatgatg ccgaagtcga gcgcgacgta gcggccgtcg ggcgtcacca
     1201 ggatgttgcc ggggtgcatg tcggcgtgga agaagccgtc gcggaacacc tgcgtgaaga
     1261 agatctccac gccggcccgc gcgaggcggg ggatgtcgac gccttgcgcc cgcagcgcgg
     1321 ccacctgcga gaccggcgtg ccgtgcatgc gctgcatcac catgacttcg ctcgagcagt
     1381 agtcccagtg gacctgcggc acgaggagca ggggcgagcc ctcgaaattg cgcttgagct
     1441 ggcttgcatt ggaagcctcg cgcagcaggt cgagctcgac ctcgagatgc ttggcgaatt
     1501 cgtcgaccac ttcgtgcggg cgcagccgcc tgccgtcgga ccacagccac tcgatcaacg
     1561 tcgcgcccgt gtgcagcagc gcgaggtccc cggcgatcac ctccgtgctg ccgggacgca
     1621 gcaccttgac cgcgacctcg gtgccgtcgg gcaggcgcgc gagatgcacc tgggcgacgg
     1681 aggcgctcgc gacgggtaac gagctcaaat ccctgaacac ctgtccggcg ggcctgccgt
     1741 agacgcggtg gagtatcgcc atgacctgct cggcggggaa cggcggcacg cggtcctgga
     1801 gcttcgccag ctcgtccgcg atgtcgggcg gcagcaggtc gcggcgcgtg gacagcacct
     1861 ggccgaactt gacgaagatc ggtcccagcg cctccagcgc ctgacgcagc cgcaccgcgc
     1921 gcggctccgc cagcgtccgc cagaagaagg ccgcgccgaa cagggcacgc agcccgcgca
     1981 cccgctcgtg gccgaggaaa aactcgtcga gcccgtagcg gacgctgacg gagagtattt
     2041 tcagcaatcg aaacaagtgc atgaggcgat tgtaaccgcc gggacgccaa ccacgccaaa
     2101 cagcgtta
//
LOCUS       DBNGWZ010000305         9162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_160791, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000305 DBNGWZ010000000
VERSION     DBNGWZ010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_160791"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2474)
                     /locus_tag="AC16CX_08935"
     CDS             complement(<1..2474)
                     /locus_tag="AC16CX_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3012673.1"
                     /translation="MLALLIILAAIAVAWVLAYHRAPALAWIVAAAIGLGLLLPYSGW
                     PQALVVACWVAFAVVAVLLYPWAIRRALVSRPLLSLFRKILPPMSQTERDAIEAGTVW
                     WDGDLFSGNPDWNKLLAYPRPQLSAEEKAFIDGPVEELCRMVHDWEITHELHDLPPHV
                     WQFIKDKGFLGMIIPRQYGGLGFSALAHSEVVMKLSTRSGTTAVSVMVPNSLGPAELL
                     LHFGTEQQKNYYLPRLAKGLEIPCFALTSPEAGSDAGAIPDFGVVCRGEWEGKKDVLG
                     IRLTWEKRYITLGPIATLLGLAFRLYDPDHLLGPEDDIGITLALIPTKTPGVHIGRRH
                     LPLNAVFMNGPNWGKDVFLPMEYVIGGAGYVGEGWKMLMNCLAAGRSISLPALSAATG
                     KMGALTTGAYGRVRQQFRLSIGRFEGVEEAIARIGGNAYIMEAARVMTAGAVDLGQKP
                     SVVSAIVKYHLTERGRQVIIDAMDVHGGKGICMGPNNYLARVYQQTPIAITVEGANIL
                     TRSMIIFGQGAIRGHPHVLKEMLATREPDPGKALRDFDAAFFGHLAFTASNKARAFWM
                     GLTGARFVSAPGDRHTRRYYQQLTRLSAAFAWTADVCMFLLGGALKRRERLSARLGDI
                     LSNLYLASAALKRYEDYGRPAEDLPLLHWSVQDALARIEDAFYGLFANLPNRFIAWAM
                     RGIIFPFAYPYGREFEPPRDHLGHQVVGLLLQPGPARDRLTAGVFIPTDPEEPIAALE
                     AALRAVIAAEPIGAKIRGARERGLITAQFADQIVDEAAAKGVITQADKAAMERAKLLR
                     RQVIMVDDFPKDLGKTEIHQTTQPVT"
     gene            2731..5988
                     /locus_tag="AC16CX_08940"
     CDS             2731..5988
                     /locus_tag="AC16CX_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3012674.1"
                     /translation="MAQVRQRGVAFAKTTRPVIGSAVRRESLFTRLDGTSGRTVAWIS
                     GPPGSGKSTLAASYVEARGCPCIWYQIDPDDADVATFFHYLSHAARKLDDGRSRELPV
                     FAPQYADDIASFSRRFFRQLFSRAKATAVLVLDNLHEVSANSPLHAAIEAGLTQVPHH
                     CCVLITSRTEPPAALARMRVTGGMVHLGWEDLRIGPHEIAEIASLRGQSLSPEAVAKL
                     HERTQGWVAGLVLMLEHAKVSGRIAELPGDATPKVIFDYLAGEIFDRFEPKTQQFLLR
                     IACLPRMSAEVAEALAGEERAGRLLFNLTQNDYFVREVPGDEGRIYQLHPLLRDFLRS
                     RAAQVLPDAVGAAELKRAVALLRRAGHLEDAISLLAECREWGEVARIAAEEAGDMLVQ
                     GRSETLCGWLDLLPPQLIEADPRLLHARAASLMHTSPRTARRLFAEAFEGFRRAGDAR
                     GMTQSCCSAVSATILEFDDLTALDHWIHTLVELLGRGTDGAPGSVDPGAAATLLRAML
                     LRDPGNNGLERWLDYAERAARATPDTPALSAVLAELALGRAMSALFRGDFAAAEAIAD
                     TLRSRKEELPAGMGIALSIASALCHLLDGGYAAALRTARDGLATAGSEGIHAYDEWLR
                     VMAVAAALAEDDRDGARGELQALEAAGTRLRRGDQTFVHYLRAWLASLEDDAAGASRE
                     SKLALALAVELGILWLECLARVAWAQVLSSDEDRRGAEAQVRGAEAIAERMRSPLLRI
                     AAHLAAAYAALRAHDEESALGPLRAGLALGREHGLRHIVAVRPQPLAELCALALRRGI
                     EPDFARGLARAGNLAPPPSALRVKLWPRPFQICMLGGFQLLHDSAPVEFSGKGPGRPM
                     ELLKVLVALGGQNVRSDQLADALWPRVDADYAHKSFTATLHRLRRILDEDEAVILRDT
                     RLSLNPRLCWLDTWALDQVMSELDTAVRDSPAGAAGPDLRALAEEALALYRGPFLPDE
                     SEQPSYIAVREQIRSRLLRCLARVARRWEDAGTPDAAIDCYLRCVDADELGEAFYRNL
                     MLCYQRRGEHAEALATYERLRTVLSTRLKVMPSPETQAVYSSLLSQHGAKAAPRD"
     gene            complement(5996..6601)
                     /locus_tag="AC16CX_08945"
     CDS             complement(5996..6601)
                     /locus_tag="AC16CX_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012675.1"
                     /translation="MVLSSRWLARWCAALAMLMAGCVTLEQKRSAIDDINMVFRAEYE
                     AILAKNGTRVYKVTRAEAYDAVRVSLARLGMTVEAQDPVLGYVNVFAPAPRPLDLKEW
                     EQAAEADLPRTREIIGPHVGIFRHFFNFEPSGLETVISGTVVEVPAGTEVSFTARMRE
                     IAPPQSGFPRREYLPPTAVRMGLDKMWAELEREFKATYRKR"
     gene            complement(6728..8572)
                     /locus_tag="AC16CX_08950"
     CDS             complement(6728..8572)
                     /locus_tag="AC16CX_08950"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3012676.1"
                     /translation="MSEQAYPDALPAQYRMHWYTLERVLGQGGFGITYLARDTNLDQH
                     VALKEYLPVEVAARRPDGAVQSRGDGQRERYRWGLERFIQEARTLARFDHPNIVRVHS
                     VFEFNGTAYMVMRFEEGDNFGTLLERRGILPESDLLRVLLPILDGLELVHNAGFIHRD
                     IKPDNIHIRRDGSPVLLDFGSARHALGKAHTLTILVAPGYAPFEQYYSSSENQGPWTD
                     IYGLGATCYRAIAGTPPLDAITRSKGILGSTREVLVPAKTIGSGRYSGRLLAAIDHAL
                     EFAEKGRPQSISDWRKELTEDGRAATAPRAVAGLSTGPDRTGGASAQASAPPEAAGAA
                     VRGAGSTVADAAAHTGWSGARAQLVWAVVGAAAAAIAIAVFLRMENPRRAAEQQLAAS
                     KQQLEQERKRFDEERRRDEEKRRADEQARKRFEEQQKQEEAKRRQEEQARLAEQQKRD
                     EEKRRLKEQQRKQALTERAPKAVAKVEAARPAVASPPASPAEPKRSEIPAVVATAKPE
                     APAPSPQAEQLARGESALARGDYSVALENLRPLAQSGNTRAQIRLAEMYASGQGVKRN
                     YNQAYIWYSLAARGGSTTAPADRDRMAKLLQPAEIKQADKVVESMRAR"
     gene            8909..>9162
                     /locus_tag="AC16CX_08955"
     CDS             8909..>9162
                     /locus_tag="AC16CX_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012677.1"
                     /translation="MSTHPGAVSCPAGSRTRGCDAGRAPHAGSRAAPSRGLFKRLRPL
                     LAPTLDAPTAPPHVKWCVYALVLLIPGSFLILPLFWLVRRL"
BASE COUNT         1436 a         3119 c         3009 g         1598 t
ORIGIN      
        1 aacgtgaccg gctgcgtggt ctggtggatt tcggtcttgc cgaggtcctt cggaaaatcg
       61 tcaaccatga tgacctggcg gcgcagcagc tttgcccgct ccatcgcggc cttgtccgcc
      121 tgcgtgatca cccctttcgc ggctgcctca tccacgatct gatcggcgaa ttgggcggtg
      181 atcagcccgc gctcgcgcgc cccgcggatc ttggcgccga tcggctcggc agcgatcacc
      241 gcgcgcagcg ccgcttccag cgccgcaatc ggctcctccg ggtcggtggg gatgaagacg
      301 ccggcggtca gccggtcgcg cgccggcccg ggctgcaaca gcagtcccac gacctggtgg
      361 ccgagatggt cgcgcggcgg ctcgaattcc cggccgtagg gataagcgaa gggaaaaatg
      421 atcccccgca tcgcccaggc gatgaagcgg ttgggcaggt tcgcgaacaa tccatagaac
      481 gcgtcctcaa tgcgggcaag cgcgtcctgc accgaccagt ggagcaacgg caggtcctct
      541 gctgggcgtc cgtagtcttc gtagcgcttc agtgccgcgg aagcgaggta gaggttggaa
      601 aggatgtcgc ccaggcgcgc cgagaggcgc tcgcggcgct tcaacgcgcc cccgagcagg
      661 aacatgcaga cgtccgcggt ccacgcgaaa gcagccgaaa ggcgcgtgag ctgctggtag
      721 tagcgccgcg tatgccggtc tcccggcgcg ctcacgaagc gggcgccggt caatcccatc
      781 cagaacgcgc gcgccttgtt cgaggcggtg aaagcgagat gcccgaagaa cgccgcgtcg
      841 aaatcgcgca gcgccttgcc ggggtcgggc tcgcgcgtcg cgagcatttc cttcagcaca
      901 tggggatgcc cgcggatcgc gccctgcccg aagatgatca tcgagcgcgt caggatgttg
      961 gcgccctcca ccgtgatcgc gatcggcgtc tgctggtaga cgcgcgcaag gtagttgttc
     1021 ggccccatgc agatgccctt gccgccgtgg acgtccatcg cgtcgatgat cacctgccgg
     1081 ccgcgttcgg tcaggtggta cttgacgata gccgacacca ctgaaggctt ctggccgagg
     1141 tctaccgcgc ccgcggtcat cacacgcgcc gcttccatga tgtaggcatt gccgccgatc
     1201 ctcgcgatcg cctcctccac gccctcgaat cggccgatgg aaaggcggaa ctgctggcgc
     1261 acgcgcccgt aggcgccggt ggtgagcgcg cccatcttgc cggtcgcggc cgaaagcgcc
     1321 ggcagcgaga tcgagcgtcc ggccgcgaga cagttcatca gcatcttcca gccttcgccg
     1381 acgtagcccg cgccgccgat cacgtactcc atcggcagga acacgtcctt gccccagttc
     1441 gggccgttca tgaacacggc gttcaacggc aagtgccggc gcccgatgtg cacgcccggc
     1501 gtcttggtgg gaatcagcgc gagcgtgatg ccgatgtcgt cctccggccc gagcaggtga
     1561 tccgggtcat agagccggaa cgcgagcccg agcagcgtcg cgatcggccc cagcgtgatg
     1621 tagcgcttct cccaggtgag gcggatgccc agcacgtcct tttttccctc ccactcgccg
     1681 cggcacacga cgccgaaatc ggggatcgcg cccgcgtccg acccggcctc ggggctggtg
     1741 agcgcaaagc acgggatctc gagccccttg gcgagtcgcg gcaggtaata gttcttctgc
     1801 tgctcggtgc cgaaatgaag caggagctcg gcgggcccga gggaattggg caccatcacg
     1861 gaaacggcgg tcgtgccgct gcgcgtggag agcttcatca ccacctcgga gtgcgcgagc
     1921 gcggagaaac cgagcccgcc gtactgcctg gggatgatca tgccgagaaa gcccttgtcc
     1981 ttgatgaact gccagacatg cggcggcaga tcgtggagtt cgtgcgtgat ctcccagtcg
     2041 tgcaccatcc ggcacagctc ttccaccggg ccgtcgatga acgccttttc ttcggcggag
     2101 agctgcggtc tgggatacgc cagcaacttg ttccagtcgg ggttgccgct gaagagatcc
     2161 ccgtcccacc acaccgtgcc cgcctcgatc gcatcccgct cggtctgcga catcggcgga
     2221 aggatcttgc ggaagaggga gagcagcggc cggctcacca gcgcgcggcg gatggcccag
     2281 gggtacagca gcaccgcaac aaccgcgaac gccacccagc acgccaccac caatgcctgg
     2341 ggccatccgg agtagggcaa aagcagcccg agcccgatcg ctgcggctac gatccaggcg
     2401 agcgcgggcg cacggtggta agccagcacc caggcaaccg cgatcgcggc taagatgatc
     2461 agtagtgcca acatgattgc ccctttgagt gagccgacgc gaacggaccg gcgttttgtg
     2521 gttggttcat gccagcatac acgggcggat ttgacgcggc aaccgcgtca aacctgtaac
     2581 tcattgacgc aataagattt tacgttgcgc acggcggaac ggccgcgcca cgcgtggacg
     2641 tttcacctca tccattgccg acaggcctgt ttttgactgc tcgcgagcgg gcgtgcgaag
     2701 atcaaatccg ggcgacgggg gaggaaccgg gtggctcagg tcagacagcg cggcgtcgcc
     2761 ttcgcgaaga cgacgcggcc ggttatcgga agcgccgtgc ggcgtgagtc gctcttcacg
     2821 cgccttgacg ggacttccgg tcgaaccgtg gcatggatct cggggcctcc cggctccggc
     2881 aagagcacgc tcgcggcgag ctacgtggag gcgcgcggct gtccatgcat ctggtaccag
     2941 atcgatccgg acgatgccga cgtcgccacg ttcttccatt acctgagcca tgccgcgcgc
     3001 aagctcgacg atggccggtc gcgggaactc ccggtgtttg cgccgcagta cgcagatgac
     3061 atcgcgtcgt tctcgcgcag attcttccgg cagctgttct cacgcgccaa ggcgaccgcc
     3121 gtcctggtgc tcgataatct gcacgaggta tcggccaaca gtccgctcca tgcggcgatc
     3181 gaagcgggcc tcacgcaggt tccccatcat tgctgcgtgc tgatcaccag ccggaccgag
     3241 ccgccggcgg cgctggcgcg aatgcgcgtg accggcggaa tggttcacct cggttgggag
     3301 gacctgcgca tcggcccgca cgagatcgcc gaaatcgcga gcctgcgcgg ccagtcactc
     3361 tcaccggagg cggtggcgaa gctccacgag cgcacgcagg gctgggtggc cgggctggtg
     3421 ctgatgctcg aacatgcgaa ggtttccggc cgcatcgccg agttgccggg cgacgcgacc
     3481 ccgaaagtga tcttcgatta cctggccggc gaaatattcg accgttttga gccaaagaca
     3541 cagcagttcc tgctgaggat agcctgcctg ccgcggatga gcgcggaggt cgccgaggcg
     3601 ctggccggcg aggaacgagc cgggcgcctg ctgtttaatc tgacacagaa cgactacttc
     3661 gtgcgcgagg ttccagggga cgaaggccgc atctatcagc tgcaccccct cctgcgggac
     3721 ttcctgcgca gccgcgcggc ccaggtgtta cccgacgccg tgggcgccgc tgaattaaag
     3781 cgcgccgtgg cgttattgcg ccgcgccggg catctcgagg acgcgatctc tttgcttgcg
     3841 gaatgccggg agtggggcga agtcgcccgg atcgccgcgg aagaggctgg cgacatgctc
     3901 gttcagggtc gcagcgaaac gctctgcggc tggctggatt tgctgccccc gcagctgatc
     3961 gaagcggacc cgcggctgct ccatgcccgt gccgccagcc tcatgcacac aagccccaga
     4021 acggcacggc gtctgttcgc agaggccttt gaaggttttc gccgggccgg cgacgcgcgc
     4081 gggatgacgc aaagctgttg cagcgcagtc agcgcgacca ttctggagtt cgacgacctc
     4141 accgcccttg atcactggat acacacgctc gtcgagctgc tcggcagggg cacggacggg
     4201 gcgcccggca gtgttgatcc tggcgccgcc gcgacgctgc tgcgtgcgat gctgctgcgt
     4261 gaccccggca acaacgggtt ggagcgatgg ctcgattacg ccgagcgcgc cgcacgcgca
     4321 acccccgaca cgccggccct gtccgcggtc ctggcggagt tggcgttggg acgcgcgatg
     4381 tccgcgctct ttcgcggaga cttcgcggcc gccgaggcga tagccgacac gctgcgatcg
     4441 aggaaggaag aacttcctgc gggcatgggg atcgcgctca gcatcgcgtc cgccctctgc
     4501 cacctgctgg acggcgggta tgcagcagca ttacgtaccg cccgcgacgg ccttgcgacc
     4561 gcgggttccg aaggtatcca tgcatacgac gaatggctcc gtgtaatggc ggtggccgca
     4621 gcgcttgccg aagacgaccg cgacggcgcc cgcggcgagc tgcaggcgct cgaggccgca
     4681 ggcacccgcc tgcgccgcgg cgaccagaca ttcgtccatt acctgcgggc ctggctcgcg
     4741 tcgctggagg acgacgcggc cggcgcgagc cgggaatcga agctggcact cgcgctcgcc
     4801 gtcgagctgg gcatactttg gctcgaatgc cttgcccgcg tggcttgggc ccaagtgctg
     4861 tccagtgatg aagatcgccg gggcgccgaa gcgcaggttc gcggcgctga agccatcgcg
     4921 gagcgcatgc gcagcccgct tctccgcatc gccgcgcatc ttgccgcggc ctacgccgcg
     4981 cttcgcgcgc acgacgagga atccgcgctc ggcccccttc gggccgggct ggcgctcggc
     5041 cgcgagcacg gattgcgtca catcgtcgcc gtgcgcccgc agccgctggc ggagctgtgc
     5101 gcgctcgcac tgcgcagagg catcgaaccc gatttcgcgc gcgggctggc gcgcgccggc
     5161 aatctcgcac cgccgccttc cgcgctgcgc gtaaaactgt ggccacgccc ttttcagatc
     5221 tgcatgctgg gcgggttcca gctgctgcac gattcggcgc cggtggaatt ctccggcaag
     5281 gggccgggac gcccgatgga gctgctcaag gtgctggttg cgctcggcgg gcagaacgtg
     5341 cgcagcgacc agctcgccga cgcgctgtgg ccgcgcgtgg acgcggacta cgcccacaag
     5401 tccttcaccg ccacgctgca ccggttgcgg cggatactcg acgaggacga agcggttatc
     5461 ctgcgcgaca cgcgcttgag cctcaacccc aggctatgct ggctggatac ctgggcgctt
     5521 gatcaggtta tgtcggaact ggacactgcg gtgcgcgact cgccggcggg cgccgcggga
     5581 cccgacctgc gcgctctcgc ggaagaagcg ttggcgctct accgcgggcc gtttctcccc
     5641 gacgaatcgg agcagcccag ctacatcgcc gtccgcgagc agatccgctc gcggctgctg
     5701 cggtgcctcg cgcgggttgc gcggcgctgg gaggatgccg gaacgcccga cgccgcaatc
     5761 gattgctacc tgcggtgcgt tgacgcggac gagctcggcg aggccttcta tcggaacctg
     5821 atgctgtgct accagcgccg cggcgagcac gcggaagcgc tcgcgaccta tgagcgcctg
     5881 cgcacggtgc tgtctacgcg gctcaaggtc atgccgtcgc cggaaaccca ggcagtttac
     5941 tcgagcctgc tctcgcagca cggcgccaag gccgcccccc gcgactgacg cggcttcagc
     6001 gttttcgata cgtcgccttg aattcccgct cgagctcggc ccacatcttg tcaagtccca
     6061 tccggacggc ggtcggcggg aggtactccc tgcgggggaa gccggactgc ggcggcgcga
     6121 tttcgcgcat gcgcgccgtg aacgagacct ccgtgccggc cggcacttcg acgaccgtcc
     6181 cgctgatcac ggtctcgagg ccgctcggct cgaagttgaa gaaatggcgg aatatcccca
     6241 cgtgtggccc gatgatttca cgcgtccgcg gcaggtctgc ctccgccgcc tgttcccact
     6301 ccttcaggtc gagcgggcgc ggcgcgggcg caaagacgtt gacgtaaccc agcaccgggt
     6361 cctgggcctc gaccgtcatc cccagccgcg ccaggctcac gcgcacggcg tcgtaggctt
     6421 cggcacgcgt caccttgtag acgcgtgttc catttttcgc gaggatggcc tcatattccg
     6481 cgcgaaacac catgttgata tcatcgattg cgctgcgctt ttgctccagg gtgacgcagc
     6541 ccgccatcag catggcaagc gccgcgcacc acctggcaag ccagcgactg ctcaaaacca
     6601 tgaaaacccg cgttgccatg gcgccccctc gtctcctgtg cccgttgccg gaccgggtat
     6661 cgctacgcgc gcggttcatt tttggagttt gccgcgcgcc cctccgcggg cagcgtgatc
     6721 gccggctcta gcgcgcccgc atgctttcaa ccaccttgtc agcctgcttg atttcggccg
     6781 gctgcagcag cttggccatc ctgtcgcgat cggccggagc ggtggtgctt ccacctcgcg
     6841 ccgccagact gtaccagata taagcctggt tgtagtttcg tttcacgccc tgtccacttg
     6901 cgtacatctc cgccagccgg atctgggccc gggtgttgcc ggactgggcc agcggcctga
     6961 gattctcgag cgcgaccgag tagtcgccgc gcgcgagcgc gctttccccc cgcgcgagct
     7021 gctccgcctg cggtgatggc gctggggcct cgggcttggc tgtcgcgacc accgccggga
     7081 tctcgctgcg cttcggctcg gcgggcgacg ccggaggaga cgcgaccgcg ggccgcgcag
     7141 cctctacctt tgctaccgct ttcggagcac gctcggtcag ggcttgtttg cgctgctgct
     7201 ccttcaagcg gcgcttttcc tcgtcgcgtt tttgctgctc cgcgagtcgt gcctgctcct
     7261 cctggcgccg cttggcttcc tcctgcttct gctgttcctc gaagcgcttg cgcgcctgct
     7321 cgtccgcgcg ccgcttttcc tcgtcgcgcc tgcgttcctc gtcgaagcgc ttgcgctcct
     7381 gctcaagctg ctgcttcgat gcggccaact gttgctccgc ggcgcggcgc ggattctcca
     7441 tgcgcaggaa cactgcaatc gcaatcgctg cagcagccgc gccgacgact gcccagacga
     7501 gttgcgcacg cgcgccggac cacccggtgt gcgccgcagc gtctgccact gtcgacccgg
     7561 caccgcgcac cgcggcgcca gccgcctcgg gcggcgcgga cgcctgcgca gaggccccgc
     7621 cggtccggtc cggcccggtt gagagccccg ccacggcgcg cggcgcagtc gctgcccggc
     7681 cgtcttccgt aagctccttg cgccaatccg atatggattg cggcctgccc ttctcggcaa
     7741 actcgagagc gtgatcaatg gccgcgagca gtcggccaga gtagcggcca ctgccgatcg
     7801 tttttgccgg aacgagaacc tcgcgcgtgc tgcccagtat gcccttgctg cgtgtgatcg
     7861 cgtcgagcgg aggcgtgccg gcgatcgcgc ggtagcaagt cgcgccgagc ccatagatgt
     7921 cggtccacgg gccctggttc tccgagctgc tgtagtactg ctcgaaaggc gcatagccgg
     7981 gggcgaccag gatagtcagg gtatgggctt tgccgagcgc gtgtcgcgcc gacccgaagt
     8041 cgagcagcac gggacttccg tccctgcgga tgtgaatgtt gtccggcttg atgtcgcggt
     8101 ggatgaagcc ggcgttgtgc accagctcca ggccgtcgag gatcggcagc aggacgcgaa
     8161 gcaaatcgct ttcgggcagg attccgcgcc gctcgagcag cgtgccgaag ttatcgccct
     8221 cctcgaaccg catcaccatg taggcggtgc cgttgaactc gaacacggaa tgcacgcgca
     8281 cgatattcgg atggtcgaag cgcgccagcg tgcgcgcttc ctggatgaac cgctccaggc
     8341 cccaccgata acgctcgcgt tgcccgtcgc cccgggactg cacggcgccg tccggccggc
     8401 gcgccgcaac ttcgaccggc aggtattcct tgagggcgac gtgttggtcg aggttggtat
     8461 cccgtgcgag atacgtgatg ccgaaccccc cctgcccgag cacccgctcg agcgtatacc
     8521 agtgcattcg gtactgcgct ggcagcgcgt cgggataggc ctgctcactc attgcagctc
     8581 ccctgtgggg acgccttgtt atgaatatgt attgccgaaa aaggcaattt attcatcata
     8641 caacagttcg accggcgcgg ttcaaatagc tctgtcccgc cctcgatcaa gctcttcctg
     8701 gaacacgcgg cggatccctt tcgtggttcc ggcatttgta agcgatccgt aacccaccgt
     8761 tatttcatct gcaagcgggc cacgctcatt ctgcgccacg taatccggct gccatggcgc
     8821 gccgttgcgc acgcccggcg ccgcgtcagc agcttatcgg ctatgggcgt ccggcaggtt
     8881 gctagaaccg atacggagga ccacgatcat gagtacccat cctggagcgg taagctgccc
     8941 tgcaggctcc cgcacccggg ggtgcgacgc gggacgcgcg ccccatgctg gctcgcgtgc
     9001 ggcgccgagc cggggcctgt tcaaacgcct gcgtccactg ttggcaccga cgctcgatgc
     9061 tcccacagcg cccccccacg taaagtggtg cgtatacgcg ctggtgctgc tgatacccgg
     9121 ttcttttctg atccttcccc tgttctggct ggtccgccga ct
//
LOCUS       DBNGWZ010000306         3615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_286679, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000306 DBNGWZ010000000
VERSION     DBNGWZ010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_286679"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1562
                     /locus_tag="AC16CX_08960"
     CDS             <1..1562
                     /locus_tag="AC16CX_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3012678.1"
                     /translation="AFEVEWDQANPIDLEVCTRCNACIRVCPEQAIDFSYQIDLDKCR
                     AHRQCVKACGDIKAIDFERKETLHNDTFDLVLDLSRDPLIKLPQPPQGYFAPRNDPLE
                     QALAANQLLQRVGEFEKPKFFSYREKICAHSRSEIIGCTRCIDVCSTRAIRSEKEENR
                     VTVEPHLCMGCGGCATVCPSGAMSYAYPRVTDIGVRIKTVLQTYREAGGRDPVLLFHN
                     TTDGRELIARLARRGRGLPANVIPLEVFHIASLGLDVMLGCVALGATRFLILSTRAEP
                     EDYLTALRGQLDYGQEILSGLGYGGRHLELIEAQDVPALERAVWNLGTVQGLPPAAFN
                     LSNEKRTTLDFVFDHMLKHAPAPKEELALSAGAPYGRVIVNKQTCTLCMACVGACPEG
                     ALLDAKEAPQLKFIERNCVQCGLCEKTCPEDAISLAPRLLLGKQAQSAVVLNEAEPYN
                     CVRCGKPFATRQMIESMVGRLSTHSMFASEPARRRLQMCADCRVIDMMEDKKEMNIFD
                     YPGGGGGGEAR"
     gene            1559..2176
                     /locus_tag="AC16CX_08965"
     CDS             1559..2176
                     /locus_tag="AC16CX_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorD/DmsD family molecular chaperone"
                     /protein_id="HIR3012679.1"
                     /translation="MSGESRTTFGSALSPEDEARANLYALAGRLFFDAPDSILLAEIC
                     REGTEPVEDDGPVSRAWRTLRAACKSAYPVVLKQEYDTLFVGVGRAEVTPYTSHYITD
                     GSPDLHLVRLREQLGLWGLARRNAAYEAEDHVSGICDVMRLLVIEGHALEDQRMFFDK
                     FAYPGVIPFCDAVATAVSAVFYRHVAEFARAFLEVEKAAFDMQED"
     gene            2342..2536
                     /locus_tag="AC16CX_08970"
     CDS             2342..2536
                     /locus_tag="AC16CX_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HIR3012680.1"
                     /translation="MKSPQRKLSRRSFLLAVGAGGTAATAALVAKDGDKSSRSAAAVG
                     ESKGYRLTEHVRKYYSTAKV"
     gene            2549..>3615
                     /locus_tag="AC16CX_08975"
     CDS             2549..>3615
                     /locus_tag="AC16CX_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3012681.1"
                     /translation="MLLTRKSTSVSASPSRLTRNVAGMLGKTIDRRTFLKRSGITLGA
                     GAVATQLPFNLVQKAEAAQLAASGIEVKRTVCTHCSVGCAIDAVVQNGVWIRQEPVFD
                     SPINLGAYCTKGAAVRDHATTHDSHRLKYPMKLAGGKWQRISWDQALKEVSDKLLAIR
                     KESGPDATYWIGSSKHSNETSYMFRKFVSMFGTNNMDHQARICHSTTVAGVANTWGYG
                     AMTNSYNDEQNSKCILFFGSNAAEAHPVSMLHTLHAKENGAKVIVVDPRFTRTAAKAD
                     TYYRIRSGTDVAFLMGMLHHIFKNGWEDKEYIKARVYGMDKVRDEAAKWTPDKVEEVT
                     GMKEAEVRDVAETMAKNRPST"
BASE COUNT          759 a         1059 c         1122 g          675 t
ORIGIN      
        1 gcgccttcga ggtggaatgg gatcaggcga atcccatcga cctcgaggtc tgcacccgct
       61 gcaacgcgtg catccgggta tgcccggagc aggcgatcga tttctcctac cagatcgacc
      121 tcgacaagtg cagggctcat cgccagtgcg tgaaagcctg cggcgatatc aaggcgatcg
      181 acttcgaacg aaaagagact ttgcataacg acacgttcga cctggtcctc gacctgtccc
      241 gcgacccgtt gatcaagctg ccgcagccgc cccagggcta tttcgcgccg cgcaatgatc
      301 cgctggagca ggcgcttgca gcgaaccagc tgctgcagcg ggtaggcgaa ttcgaaaaac
      361 cgaaattttt ctcctaccgg gaaaagatct gcgcgcacag ccgctccgag atcatcggtt
      421 gcacccgctg catcgacgtt tgctcgacgc gcgcgatccg gagcgaaaag gaggaaaacc
      481 gcgttacggt cgagccgcac ctgtgcatgg gctgcggcgg ttgcgccacc gtttgtccgt
      541 cgggcgcgat gagctacgct tatccgcgcg tgacggacat cggagtaaga atcaagacgg
      601 ttttgcagac ctatcgcgaa gcgggcgggc gcgatcccgt gctgttgttt cataacacaa
      661 cggacggccg ggagctcatt gccaggctgg cccgccgcgg ccgtggcctg ccggcaaacg
      721 tgatcccgct cgaggttttt cacatcgcct ccctcggcct cgatgtcatg ttgggctgcg
      781 tggccctcgg cgcgacccgg ttcctcatcc tgtccacgcg cgccgaacct gaggactatc
      841 tgacggcgct gcgcggccag ctcgactatg gccaggagat cttgtcgggt ctgggatatg
      901 gcgggaggca tcttgagctg atcgaggccc aggacgttcc tgcgctggaa cgggcggttt
      961 ggaacctcgg aactgtgcag gggctcccgc cagccgcctt caacctgtcc aacgagaagc
     1021 gcaccacgct cgatttcgtc ttcgatcata tgctcaagca tgcgccggct cccaaggagg
     1081 agttggcgct ttcagccggg gcgccgtacg gccgcgtcat cgtcaacaaa cagacttgta
     1141 ctctttgcat ggcttgcgtg ggagcctgtc ctgaaggggc gttgctcgat gccaaggaag
     1201 cgccccagct caagttcatc gagcgcaact gcgtgcagtg cggcctgtgc gagaaaacct
     1261 gtccggaaga cgcgatcagc ctggcgccgc ggctgctgtt gggaaaacag gcccaatcag
     1321 ccgtggtgct caatgaagcc gagccctaca actgcgtgcg ctgcggcaag ccgttcgcga
     1381 cccggcaaat gatagaaagc atggtgggac gattgagcac gcattcgatg ttcgcctcgg
     1441 agcccgcgcg gcgccggctg cagatgtgtg cggattgccg ggtgatcgac atgatggaag
     1501 acaagaaaga gatgaacatc ttcgactacc cgggtggtgg aggcggagga gaggcccggt
     1561 gagcggggaa tcccggacaa ccttcggcag cgcgctatcc cccgaggatg aggcccgcgc
     1621 caacctttac gcgctggccg ggcgcctttt ttttgacgcc ccggactcta ttctgctggc
     1681 tgaaatctgc cgggagggaa cggagcccgt ggaagacgat gggcccgtat cgcgggcttg
     1741 gcgcacgcta cgtgcggcct gcaagagcgc ctatccggtt gtgcttaaac aggaatacga
     1801 caccctgttc gtgggcgtgg gcagggcgga agtaactccg tatacctcgc attacataac
     1861 ggatggctct cccgatctgc acctcgtcag gctccgggaa cagttggggt tatgggggct
     1921 tgctcgccgc aatgcagcgt acgaagccga agatcacgta tcgggcatct gcgatgtcat
     1981 gaggttgctg gtcatcgagg gccatgctct tgaagatcaa cgaatgtttt tcgacaagtt
     2041 tgcctatcca ggcgtaatcc ctttctgcga cgcggtggca acagcggtgt cagccgtctt
     2101 ctaccgtcat gtggctgaat ttgcacgcgc ttttttagaa gtggagaaag cagcctttga
     2161 tatgcaggaa gactgactta gcaattagat gagaattgtt cttgttaggg cgctctccaa
     2221 ttgcgcgaaa acgaatatca ctcttagaac tcatcaaatt gtggttgatt tggcactttc
     2281 ccgagggtag atttattcct caataaataa atcttttaag cagttctgca aggaggatgc
     2341 gatgaaaagt ccgcagcgca aactttcccg ccgtagtttc ctgcttgccg taggcgcggg
     2401 cggcacagca gctaccgcag cgctggtcgc gaaagatgga gacaaatcct ccagaagcgc
     2461 cgcggcggtg ggcgaatcga agggctatcg cctgaccgag catgtccgga agtactacag
     2521 caccgccaaa gtctgaggag agagcgccat gctgctcacg agaaaatcca ccagcgtttc
     2581 cgcgagcccg agccgtctca cccgcaacgt cgcgggcatg ctcggcaaaa ccatcgatcg
     2641 caggaccttc ctcaaacgtt ccgggatcac cctgggggcc ggcgcggttg cgacgcagct
     2701 cccgttcaac ctcgtgcaga aagccgaggc cgcgcagctg gccgccagcg gcatcgaggt
     2761 caagcgcacg gtctgcaccc attgctcggt gggctgtgcg attgacgccg tggtacagaa
     2821 tggcgtgtgg atccgccagg agccggtatt tgactcgccg atcaacctcg gcgcgtattg
     2881 caccaaaggc gccgcggtcc gcgaccacgc cacgacgcat gactcgcacc gcctgaaata
     2941 tccgatgaag cttgccggtg gcaagtggca gaggatttcg tgggaccagg cgctcaagga
     3001 agtgagcgac aagctgctcg cgatccgcaa ggaaagcggt cccgatgcca cctactggat
     3061 cggctcctcc aagcacagca acgagacctc ctacatgttt cgcaagtttg tgtccatgtt
     3121 cggcaccaac aacatggacc accaggcgcg catttgccat tcgacgacgg tggcaggggt
     3181 ggcgaacacc tggggttacg gcgcgatgac caactcctac aacgatgagc agaactcgaa
     3241 gtgcattctg ttcttcggca gcaacgcggc ggaagcgcac ccggtgtcga tgctgcacac
     3301 gctgcacgcc aaggagaacg gcgccaaggt gatcgtggtg gatccacgct ttacccgcac
     3361 cgcggcgaag gcggacacgt attaccgcat ccgctccggc accgacgtcg cgttcctgat
     3421 gggcatgctg caccacatct tcaagaatgg ctgggaggac aaggaataca tcaaggcgcg
     3481 cgtctacggc atggacaaag tgagggacga agcggcgaag tggacgccgg acaaggtcga
     3541 ggaagtcacc ggcatgaagg aagcggaagt gcgcgacgtt gccgagacga tggccaagaa
     3601 caggcccagc acgat
//
LOCUS       DBNGWZ010000307         5900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_161091, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000307 DBNGWZ010000000
VERSION     DBNGWZ010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_161091"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16CX_08980"
     CDS             <1..203
                     /locus_tag="AC16CX_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR3012682.1"
                     /translation="QTGQYGDMVEMGVLDPTKVARCALQNAASVAGLILTTDAMVAEL
                     PKDESPMGHGHGGMGGMGGMDM"
     gene            1025..1100
                     /locus_tag="AC16CX_08985"
     tRNA            1025..1100
                     /locus_tag="AC16CX_08985"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1058..1060,aa:Phe,seq:gaa)
     gene            1403..2620
                     /locus_tag="AC16CX_08990"
     CDS             1403..2620
                     /locus_tag="AC16CX_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3012683.1"
                     /translation="MQNIVIAKPYRFVPPHRGTFWPGLLLRCVPLYLRACHGVTRVIC
                     RGVDRLRASIEAGHGIVLAPNHCRPCDPMVVAVLAGQVRRYLYIMASWHLFMQNRFQA
                     WLLTRAGVFSVHREGADRESLKHATELLSEADRMMLIFPEGVISRNNDRLNILMEGAA
                     IMARAAARLRAGRTSPRRVVIHPVAIRYFFDGDLESAITPVLEDIEARMAWRPQRDLP
                     LIERVLKVGSAMLALKEIEYFGEPQRGEVGERVQRLVDRLLCPLEQEWLQGQRQGDVV
                     RRVKSLRKAMLANMAGGKLSPVEQDRRERHLADIYLAQQLAFYPAGYFSPEPTPERIL
                     ETVERFEEDTTDVARVHSPMRAVVEVGEAIEAPPEEQRDSDGDPLMGEVRVQLTALLA
                     ASLAEVHPNATMR"
     gene            2626..3261
                     /locus_tag="AC16CX_08995"
     CDS             2626..3261
                     /locus_tag="AC16CX_08995"
                     /inference="COORDINATES: protein motif:HMM:NF048897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HIR3012684.1"
                     /translation="MAEILIRTAVIGVALAGWFWTQHLLSRRAVAAGIRDHVHEWTSP
                     LHAWLAAHPRATDVTLIVSSALIDLIGLYLIAAAIFGPSFRPFIALFVLFVLRQMCQG
                     LCALPTPAGSIWRYPGFPALLVTYGTDNDYFFSGHTAVAVLGAIELVHAGPPWLALFI
                     CLIAVLEGATVLVLRAHYTMDVFAATLAAFFSYNLGAATAPAVDSWIASMF"
     gene            complement(3522..4487)
                     /locus_tag="AC16CX_09000"
     CDS             complement(3522..4487)
                     /locus_tag="AC16CX_09000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3012685.1"
                     /translation="MPAQKSHVAIIGGGIMGGDIAIIFAAGGWNVHVMSPSQKTRDAL
                     PARVKAGLKKLTAPPANAAGVKTCARLEDLPWRDIGIVVEAVVEDLPLKQRLFGEIEM
                     LARPDIPLASNTSNFPIGEIGRALKHRVRVAGLHFFMPAHLVPLVEIVSSEFTDPGIA
                     EGLVALMKNLGKAPIWVKKDVPGFVGNRIQHAMMREALYLIADGVVSPEDVDTAVRYG
                     FGFRFIACGPIMQKEMSGWDTNVLVGSALYPHLHAESAYPALVKDMVDKGHLGMKTKR
                     GFWLWTDESIAREKTRIERCLQAGMEILKSDAKESGTQGRKRRAR"
     gene            complement(4608..5480)
                     /locus_tag="AC16CX_09005"
     CDS             complement(4608..5480)
                     /locus_tag="AC16CX_09005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3012686.1"
                     /translation="MHSLKLHAPRFFAALLATVALALPAAAPAQNKEYTPQVGQEGKD
                     VIWVPTPEALVEKMLDMAKLTPNDIHYDLGSGDGRTVIAAAKRGARAVGVEYNPDMVA
                     LSERAAAKEGVTGKVKFIRGDIFETDFSEATVLTLYLLPSLNLKLRPTILRMKPGTRV
                     VSHAFSMDDWQPDQTENVEGRTAYLWIVPAPVEGNWRWSVSGSGPKEYELALRQQFQK
                     VEGLIRLNGKMGQLRDVKLQGDQISFSVHEVAGTSGTLRRDFTGRVSGNTMQGVVKLP
                     NSAGEAKWTATRVN"
BASE COUNT          988 a         1858 c         1898 g         1156 t
ORIGIN      
        1 cccagaccgg ccagtacggc gacatggtgg agatgggcgt gctcgatccg accaaggttg
       61 cgcgctgcgc gctgcagaac gcggcctcgg tggcgggcct gatcctcacc accgacgcga
      121 tggtcgcgga gctgccaaag gacgagtccc ccatgggcca cggccacggc ggcatgggtg
      181 gcatgggtgg aatggacatg tgacgagcgc gtcagcgcga gtcatccccg cgccagaggt
      241 acacccccga atgatttagt cgggggcggg gatccagcaa cacagaaagc cccgcacgag
      301 cggggctttt tgtttgtatg cgagcgtaga ttcgcagctc tttcttgacg ctcgcggcga
      361 ttaacgctta aataccgccc ttcgtttttc tacggggccc ggatagttgc gttggtggag
      421 cagaggactg ataacatggc cgttttttgg catgttcggg catagactaa gtcatgcagc
      481 gaagttaagt aagcgctcgc tcacgctccg aattagtatc cttatgtcag gcggttcgca
      541 gtaacgtttt gcagcaagtg gccgggttgc tcgaacattc cgatgcggaa atggttgggt
      601 tccatttccg cacagtccat ttcccctcgc cctccgggag agggagaggg tgcgccgaaa
      661 cgtggagcag gggccccgtt gcttggggat gcgaccgtgg aggcgcacgt aagcacgcgg
      721 gcaattgcgg cgccgcatcc cgctccgggg tcgcatccaa gcacaagacg cttggaacct
      781 gctcagccct ccgcgggcgc aggggtgagg gtaaggtaga gcagaggact gacaacacgc
      841 cgcgcgagac ttcgcttcag cgaagtctga ccgcaggtca aatgggggcc ttatcccatt
      901 gcgcggcttg ttgcggcgta ggctaatatt cacggcggaa acgccgttaa tgttaagtgc
      961 cgggaggcac ggccggagcc aaaatctttg tgtcaggcgg ttcaacggtt tttcgcagtc
     1021 aagtggccgg gtagctcagt tggtagagca gaggactgaa aatccttgtg tcggcggttc
     1081 aattccgtcc ccggccacca tttccttccg tagttctggc aactctgcta cccggccgcc
     1141 tgtcgttggt aacttcgtcc catagtagta aagcattgct gcgcgatctt tacgcatcaa
     1201 tctcaatcgc gtagattgtt gcgcgatact cgattgccgg aggccgtgcc cggccgaatc
     1261 gggtaactgc cggcagatcc agcgcgcacc ggagccgctc cgcccgcccg gcggcacggg
     1321 tgcggcacag gccctccgaa ctcgccactg ccgcggcgtc aagatttttt tgctcctctt
     1381 gtgcttgccg gacaggattg ggatgcaaaa catagtcatc gcaaaaccct accgctttgt
     1441 gccgccgcac cgcggcacat tctggccggg actgctgctg cgctgcgtgc ccctctacct
     1501 tcgcgcctgc cacggcgtga cccgcgtgat ctgccgtggc gtggatcggc tgcgagcctc
     1561 catcgaggca ggccatggca tcgtgctcgc gcccaatcac tgccggccct gcgaccccat
     1621 ggtggtcgcc gtcttggcgg ggcaggtgag gcggtatctg tacatcatgg cgagctggca
     1681 cctgttcatg cagaatcgtt tccaggcatg gctgctgacg cgcgccggcg tgttcagcgt
     1741 ccaccgcgaa ggcgcggacc gcgaatcgct aaagcacgca accgagctgt tgagcgaggc
     1801 cgaccggatg atgctgattt ttcccgaggg cgtcatttcc cggaataacg accgactcaa
     1861 cattctgatg gagggtgccg cgatcatggc tcgcgcggcc gcccgcctgc gcgccggccg
     1921 gacttctccg aggcgcgtcg tgatccatcc cgtcgcgatc cgctatttct tcgatggcga
     1981 tctggaaagc gcgatcaccc cggtgctcga ggacatcgaa gcgcgcatgg catggcggcc
     2041 gcaacgggat ctgccgctca tcgagcgcgt gctcaaggtt ggcagcgcca tgctcgcgct
     2101 caaggaaatc gagtatttcg gcgaaccgca gcgcggagag gtcggcgagc gcgtgcagcg
     2161 cctcgtcgat cgcctgctct gcccgcttga gcaagagtgg ctgcagggac agcgccaagg
     2221 cgatgtcgtc cggcgcgtca agtcgctgcg caaggccatg ctggccaata tggctggcgg
     2281 caaattgtcc ccggtggagc aggaccgccg cgaaaggcat cttgccgaca tctaccttgc
     2341 gcaacagctg gcgttctacc cggcgggcta cttcagcccg gagcccacgc cggagcgcat
     2401 tctcgaaacc gtcgaaaggt tcgaggagga tacgaccgac gtcgcgcgcg tgcactcgcc
     2461 catgcgcgct gttgtggagg tcggcgaagc tatcgaggcg cccccggagg agcagcgcga
     2521 cagtgatggc gacccgctca tgggagaagt ccgggtgcaa ttgaccgccc tgcttgccgc
     2581 gtcactggcg gaagtacacc cgaatgccac catgcggtga cgaccatggc cgaaatcctg
     2641 attcgtaccg ctgtcatcgg tgtggccctg gcggggtggt tctggacgca gcacctgctg
     2701 agccggaggg ccgtggccgc cggcatcagg gaccacgtgc acgagtggac atcgccgctg
     2761 cacgcgtggc tggccgcgca tccgcgggcg acggacgtca cgctcatcgt cagttccgcg
     2821 ttgatcgact tgatcgggct atacctgatc gccgcggcca ttttcggccc gagcttccgg
     2881 cccttcatcg cgctgttcgt gctgttcgtc ctgcgacaga tgtgccaggg attgtgcgcg
     2941 ctgcccacgc cggccggatc gatctggcgt tatccgggat ttccggcatt gctggtcacc
     3001 tacggcacgg acaacgacta cttcttctcg ggtcacacgg ccgtcgcggt cctcggcgca
     3061 atcgaactcg tccatgccgg cccgccatgg cttgcgcttt tcatatgcct gattgccgtg
     3121 ctggaaggcg caacggtgct ggttctgcgc gcgcactaca cgatggatgt gttcgccgcg
     3181 accctggccg catttttttc ctacaatctc ggcgcagcca cggcaccggc ggtcgatagc
     3241 tggatcgcgt ccatgttctg aatcggtccc gccggcacga cagcagcaac ggctctggcc
     3301 gttttattcg gttcgagcag atctgactga aagttctcat gccagaagct caatttggct
     3361 ccgacatcgc accgcgcgct gcgcggtatc agtccccgcc gcaacaaggt gtgaaacagg
     3421 ctttcgcgat gaccgactaa aacccggtct ccgcgaccag gattgaccgc gcggcgcgct
     3481 gtcgtccctg gccaccattc cccttggtct tggcaactga actacctagc ccttcgtttt
     3541 cgtccctggg tgccgctttc ttttgcgtcc gatttcagga tctccattcc cgcctgcagg
     3601 caacgctcga tgcgcgtctt ctcccgcgcg atcgactcgt ccgtccacag ccagaagccg
     3661 cgcttcgtct tcatgccgag gtggcccttg tccaccatgt ccttgacgag ggcggggtag
     3721 gccgactcgg cgtggaggtg ggggtagagc gcgctgccga cgaggacgtt ggtgtcccag
     3781 cccgacatct ccttctgcat gatcggaccg caggcgatga agcggaagcc gaagccgtag
     3841 cgcaccgccg tgtccacgtc ttccggcgag acgacgccgt cggcgatgag gtagagcgcc
     3901 tcgcgcatca tcgcgtgctg gatgcggttg ccgacgaagc cgggcacgtc cttcttcacc
     3961 cagatcggcg ccttgcccag gtttttcatg agagcgacca ggccctcggc aattcccggg
     4021 tcggtgaact cagagctcac gatttccacc agcggcacga ggtgcgccgg catgaagaag
     4081 tgcaagcccg cgacccgcac gcggtgcttc agcgctcttc cgatttcccc gatggggaag
     4141 ttcgaggtgt tgctcgcgag cggaatgtcg gggcgcgcga gcatctcgat ctcgccgaac
     4201 agcctctgct tgagcggcag gtcttcgact acggcctcga ccacaatgcc gatgtcgcgc
     4261 cacggcaggt cctcgaggcg ggcgcaggtt ttcacgccgg cagcgttcgc cggcggcgcg
     4321 gtgagcttct tcagaccggc cttgacgcgc gcaggcagcg catcgcgcgt cttctgcgac
     4381 gggctcatca cgtgcacgtt ccagccgccg gcggcgaaga tgatggcgat gtcgccgccc
     4441 atgatgccgc cgccgatgat ggccacgtgg gatttctgcg ctggcatggt tcaggctccg
     4501 gattttcgac agatgaccgg tgtttggtcg gcggtcggca ttcagcgttc agccatcagc
     4561 ttgatcctcc gccgtcgact ccggctgaca gctgacggct gatcgctcta attgacccgc
     4621 gtcgccgtcc acttcgcttc gccggcgcta ttgggcagtt tcacgacgcc ctgcatggtg
     4681 ttgccggaaa cgcgtcccgt gaagtcccgg cgcagggtgc cgctggtgcc ggcaacctca
     4741 tgcaccgaaa agctgatctg gtcgccctgc agcttcacgt cgcgcagctg ccccatcttg
     4801 ccgttgagac ggatcagccc ttcgactttc tggaactgct ggcgcagcgc gagctcatat
     4861 tccttcgggc cgctgccgga gacgctccag cgccagttgc cctccaccgg cgccggcacg
     4921 atccacaggt aggcggtgcg gccttcgacg ttctcggtct gatcgggctg ccagtcgtcc
     4981 atgctgaagg cgtgcgatac cacgcgcgtg cccggcttca tccgcagaat ggtcggacgg
     5041 agcttgaggt tcaagctcgg cagcaggtag agcgtgagga cggtggcctc gctgaaatcg
     5101 gtctcgaaga tgtcgccacg gatgaacttc accttgccgg tcacgccctc cttggcggcg
     5161 gcgcgctcgg agagcgcgac catgtcaggg ttgtactcga cgcccacggc gcgggcgccg
     5221 cgcttggcgg cggcgatcac tgtgcggccg tcaccgctgc cgagatcgta gtgaatgtcg
     5281 ttgggcgtga gcttcgccat gtcgagcatc ttctcgacca gcgcctccgg cgtcggcacc
     5341 cagatgacgt ccttgccttc ctggccgacc tgcggcgtgt attccttgtt ctgcgccggc
     5401 gcggctgcgg gcagcgcgag cgcgacggtg gcgagcaatg cggcgaaaaa tcggggtgcg
     5461 tgcagcttca agctgtgcat gggattctcc gtggtaagtg gctaactggt tctgcggcta
     5521 tgggagttac atcgtgcggg cgcctagttt acagcacgca cgatacttct attccagcga
     5581 tgacggtacg tgcgcggaac gtgcatgggc aaggtgaagc cgtcggggcg tgagagcgtg
     5641 aggacgacaa catccgtgag ggcgggagag cgcgagagcg agagagcgag agagcgagag
     5701 agcgagagag cgagaagaaa ggcactttcc atttcccgac ttcccgacct ctcgatctca
     5761 cggcttctgc aaacggccgt ccaccgtgac gcctttggcg atcatcgctt cgatctcggc
     5821 gtcggtgcag ccgatctcgc gcaaaacctc gacgctgtgc tggccgagct tgggcgccgg
     5881 gctccattca cggcgcacgc
//
LOCUS       DBNGWZ010000308         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_787973, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000308 DBNGWZ010000000
VERSION     DBNGWZ010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_787973"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="AC16CX_09010"
     CDS             complement(<1..397)
                     /locus_tag="AC16CX_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3012687.1"
                     /translation="MKRLLAVAGVLVGFLLAHAAPASEITVLSGGAIEPGLNVAAAAF
                     QKETGNAVKITYNTTPQMRKRVAAGDTFDVVIAPPAAVKDFAAAGKVEEGGVNVGRVG
                     LGVVIRPGAPVPVISSAEAIRRTVLEAESV"
     gene            complement(509..1462)
                     /locus_tag="AC16CX_09015"
     CDS             complement(509..1462)
                     /locus_tag="AC16CX_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012688.1"
                     /translation="MVLLCAALAAVLLPATAQQYPVKPIRYIAPNLPGGPTDILARLI
                     GQKLSESMGQPVIVENRAGASGIIGTELAAKSPPDGYTLVSGNNATFGANVSLFKKLP
                     YDPIKDFSPVVFLGTQPNILVVHPSLPVKSVKELIAFARARPGQLNYAGTGMGAAANL
                     SAELFKSMTGTKMVGISYKSAAPAITDLIAGQTQLMFATSLSIMPHIQAKRVRPLGVT
                     TAKRMRAMPELPTIAEAGVPGFEASTWHGVLVPAGTPAAIIDKLNAEINRALQQPDVR
                     ERLAALGAEIIGGTPKEFADHIQREIPKWAKVIRESGARAE"
     gene            1735..>1938
                     /locus_tag="AC16CX_09020"
     CDS             1735..>1938
                     /locus_tag="AC16CX_09020"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3012689.1"
                     /translation="MKQSQRIRGVLSPVVTPFSKDLSPDPGRFIAHCRWLITQNCGLA
                     VFGTNSEANSLSVNERIALLDSLL"
BASE COUNT          331 a          649 c          572 g          386 t
ORIGIN      
        1 cgacggattc cgcttccagc acggtgcgcc tgatcgcttc cgcgctcgag ataaccggca
       61 ccggcgcgcc cggacggatc accacgccca accccacgcg cccgacgttg acgccgccct
      121 cctcgacctt gccggccgca gcgaagtcct tgacggccgc aggcggcgcg atcaccacgt
      181 cgaacgtgtc gcctgcggcg acgcgcttac gcatctgcgg cgtcgtgttg taggtaatct
      241 tgacagcatt gccggtctcc ttctgaaacg ccgcggccgc gacgttcagg cccggctcga
      301 tcgcgccgcc cgagagtacc gtgatctccg atgctggcgc ggcgtgcgcc agcaagaagc
      361 cgaccaacac tcctgcaact gccaatagcc ttttcatgtc atctccctca ttattccaaa
      421 tgcgtgataa gtgacgagtg ataagtgata aggctcccgc gcagcggaaa ttaaccccta
      481 tcacttatca cctatcacgc ctcatccctc attcagcccg cgctccggat tccctgatca
      541 ccttcgccca cttcggtatc tcgcgctgga tgtgatcggc gaattccttg ggcgtgccgc
      601 cgatgatttc cgctccgagt gcggcgagcc gctcacgcac gtccggctgt tgcagcgcgc
      661 ggttgatctc cgcattgagc ttgtcgatga tggccgccgg ggtgcctgcc ggcacgagca
      721 ccccgtgcca ggtgctcgcc tcgaagcccg gcacgccggc ctccgcgatc gtcggcagct
      781 ccggcatggc gcgcatccgc ttggctgtgg tcacccccag cgggcgcacc cgctttgcct
      841 ggatatgcgg catgatcgac agcgacgtcg cgaacatgag ctgggtttgc cccgcgatca
      901 ggtctgtgat tgccggggcg gcactcttgt aggaaatgcc caccattttg gttcccgtca
      961 tgctcttgaa gagctccgcc gacaggttgg cggccgcgcc cattcccgtg cccgcgtaat
     1021 tgagctgtcc gggccgcgct cttgcgaagg cgataagttc cttgactgat ttcaccggca
     1081 gcgacgggtg cacgaccagg atgttgggtt gtgtcccgag gaacacgacc ggcgagaaat
     1141 ccttgatcgg atcgtacggc aatttcttga acaggctcac gttggcgcca aaggtagcgt
     1201 tgttcccgct caccagcgtg tagccgtcgg gcggagactt ggcagccagc tccgtgccga
     1261 tgatgccgct ggcgccggcg cggttctcga cgatgacggg ctggcccatg ctttcggaga
     1321 gcttctgccc gatgaggcgc gcgaggatgt cggtcggccc gccggggaga ttcggagcta
     1381 tgtaacggat cggcttgaca gggtattgct gtgctgtcgc tggcagtagt acggccgcca
     1441 gcgcggcgca cagcaatacc aatgatgaac gatgaatgat gaacgatgaa cggtctggct
     1501 ccgacgggcc ggcccggcat tcacggatat cgcttcgcat cccggattcc agactcatcc
     1561 gaagggttat tcacgccacg cctcgtgcgc ggagtcgcaa gcttagcaat gggacacttt
     1621 catgacaaca cgctcatctt ctttctgcca cgcccctgtg ctattctcgg caacgactat
     1681 catccgcgga ccgcttcagg accgccgccg ttcctagaaa tcgaggagac actcatgaaa
     1741 cagtcgcagc gcatccgtgg cgtgctttcc ccggtcgtca cgccgttcag caaggacctg
     1801 agccctgatc ccgggcgctt catcgcgcac tgccgctggc tgatcaccca gaactgcggc
     1861 ctcgcggtgt tcggcaccaa cagcgaggcg aactcgctct ccgtcaacga gcgcattgcg
     1921 ctgctcgatt ccctgctc
//
LOCUS       DBNGWZ010000309         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1167427, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000309 DBNGWZ010000000
VERSION     DBNGWZ010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1167427"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC16CX_09025"
     CDS             <1..398
                     /locus_tag="AC16CX_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3012690.1"
                     /translation="PVDPVRDEVVGPAGPTTSRRMDKFMDVMLGKTGLLGSIGKSERG
                     AQALEAIKRHKGVHLIAVGGAAYLVSKAIRAARVLAFQDLGMEAIHEFEVKDMPVTVA
                     VDTTGNAAHESGPREWRARIGKIPVTVQV"
     gene            complement(650..1591)
                     /locus_tag="AC16CX_09030"
     CDS             complement(650..1591)
                     /locus_tag="AC16CX_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR3012691.1"
                     /translation="MPPVEPTLTALPNPVLRYFLATRPAFLSVTLFACLVGTGAAWYD
                     GVTLSVGKAVATLVFALLAHAGINVLNDYYDDLNGTDRVNTERVFPFTGGSRFIQNGV
                     LTARETLVYGVALIGIVIVAGLWLTSVSAAGLVWIGAAGLFIGWAYSAPPLSLNSRGL
                     GELCVALGFALVVVGTDFVQREAFAPLPAVAAVSYALLVTDILYINQFPDRRADEAAG
                     KRHWVVILGARRARWGYLAIGAIAYGFLVAAVAADTLPRLCLIALASVAPTATAGAQL
                     LKFAEKPAALAPAIQLTIAAASLHGLLLAVALAFARF"
     gene            complement(1620..2222)
                     /locus_tag="AC16CX_09035"
     CDS             complement(1620..2222)
                     /locus_tag="AC16CX_09035"
                     /inference="COORDINATES: protein motif:HMM:NF013935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR3012692.1"
                     /translation="MSEITPTRSAVIALGEERRAMHDSYVFLDEKCLLLAEAMMRELR
                     RHDELVASLRAMLKEAAAVLHAALARHGLQGLQCYPPADLRDAAPRVARQRLMGVVLQ
                     TAELEVTARPALEPVNPSPEAEACREQYARAAALAARIAAVSGNLERLYHEYRRSVRR
                     VRALQDVLLPEIDRTVYEIETRLEELEQDEALWLRHHRGT"
BASE COUNT          431 a          787 c          790 g          354 t
ORIGIN      
        1 ggccggtcga tccggtgcgt gacgaagtgg tcggccccgc cggccccacc acctcgcgcc
       61 ggatggacaa attcatggac gtgatgctcg gcaagacggg attgctcggc tcgatcggca
      121 agtcggagcg cggcgcgcag gcgctcgagg cgatcaagcg ccacaagggc gtgcatctca
      181 tcgccgtggg cggcgcggcc tacctggtgt cgaaggcgat ccgcgctgcg cgcgtgctgg
      241 cgttccagga cctcggcatg gaagccatcc acgagttcga ggtgaaggac atgccggtca
      301 cggtcgcagt ggacacgacc ggcaacgcgg ctcacgagtc cggcccgcgg gaatggcgcg
      361 cccggatcgg caagatcccg gtcaccgtgc aagtttgacg cccaaaggag tttgcgggac
      421 ctttgtcccg caaactcctt tatgcaaaac cgccctcaga aaaccgcgaa agaaggctaa
      481 gattcaccga ctgctgtcct cggctcgcag acaaacaacg ggggtaaagg ggcgtgtcgc
      541 cgccgatgac gaatttgcga aaaaatactt aagcccgcgt gtcgcaggcg cttgcccgtg
      601 tgttgtgatc gcatgacccc aaagccatgg cagtcatgaa acatgcgggc tagaaacgcg
      661 cgaaggcaag cgcgacggcc agcaacaggc catgcagcga cgctgccgca atggtgagct
      721 ggatggccgg agccagggcg gccggctttt ccgcaaactt gagaagttgc gcgcccgccg
      781 tggcggtcgg tgccacgctg gcaagggcga tgaggcagag tctcggcaaa gtgtccgccg
      841 ccactgccgc caccaggaat ccataggcga tcgcgccgat cgcaagatag ccccagcgcg
      901 cccgccgcgc accgagtatc accacccaat ggcgcttgcc cgccgcctcg tcggcgcgcc
      961 ggtcggggaa ctggttgata tacaggatat cggtgacgag cagcgcgtac gacaccgccg
     1021 ctaccgccgg cagcggcgcg aacgcctcgc gttgcacgaa atcggtgccc accacgacca
     1081 gggcgaaacc gagcgccacg cacaactcgc cgagcccgcg gctgttgagg gaaagcggcg
     1141 gcgccgaata ggcccagccg atgaagagcc ccgccgcgcc gatccagacg aggcccgcgg
     1201 cggatacgct cgtcagccac agtccggcga caatcacgat gccgatcagt gcaaccccgt
     1261 aaacgagcgt ctcccgcgcc gtgagcacgc cgttctggat gaagcggctg ccgccggtga
     1321 acggaaacac gcgttcggta ttgacgcggt cggtgccgtt caaatcgtcg tagtaatcgt
     1381 tgagcacgtt gatgccggcg tgcgcgagga gcgcgaacac cagggtcgca accgctttcc
     1441 cgactgacaa ggtaaccccg tcgtaccagg cggctcccgt cccgacgagg caggcaaaca
     1501 gcgtgacgct caggaacgcc ggccgcgtcg caagaaagta gcgcagcacc ggattgggaa
     1561 gcgcggtcag cgtgggctct acaggcggca tccggccctc tccccgtcgg cgcggtccct
     1621 catgtcccgc gatgatgcct gagccagagc gcttcgtcct gctccagctc ttcgagccgg
     1681 gtctcgatct cgtataccgt gcgatcgatc tccggcagga gcacgtcctg cagcgcgcgc
     1741 acccgcctga ccgagcgccg gtactcgtga tagaggcgct cgaggttgcc tgaaacggct
     1801 gcgatccggg cggccagcgc ggccgcccgc gcgtactgct cccggcacgc ctcggcctcc
     1861 ggcgaggggt tgaccggctc gagcgccggc cgggcggtga cttcgagttc cgccgtttgc
     1921 agtacgacgc ccatcaagcg ctgccgcgcg accctgggag ccgcgtcgcg gagatccgcg
     1981 ggcggatagc actgcaagcc ctggaggccg tgccgtgcaa gcgcagcgtg gagcacggcg
     2041 gcggcttctt tgagcatcgc gcgcagggac gccaccagct catcatggcg tctcagctct
     2101 ctcatcatcg cctcggcgag caacaggcat ttctcgtcga ggaagacgta gctgtcgtgc
     2161 attgcgcgcc gctcttcgcc gagcgcgatg acggcgctgc gggtcggcgt gatctcgctc
     2221 atggcgcgcg cccctaatga gctttcggca actggttggc gctccagttg ccgctgtcca
     2281 tttcccctct ccgcccggga gaggtgcgcc gaaacgtgga gcagggatcg gcgcaacgtg
     2341 gagcagggac ccgcttgcgg ga
//
LOCUS       DBNGWZ010000310         6738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1042640, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000310 DBNGWZ010000000
VERSION     DBNGWZ010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1042640"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1236)
                     /locus_tag="AC16CX_09040"
     CDS             complement(79..1236)
                     /locus_tag="AC16CX_09040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3012693.1"
                     /translation="MTIKVVESLSGTAAGEWNRLAGGDPFLSHEFLSALHETGCASSA
                     SGWAPQYLLLKRNGLLAGAMPLYLKAHSYGEYVFDWAWADAYYRHGVAYYPKLVSAVP
                     FTPVTGTRLLATTDEDRDRLISAALELARKLRVSSLHCLFPLRGEAQRMRAHGMMPRH
                     AVQFHWTNRGYGSFDEFLAGFSHDKRKRVRQERRKVSNTGIHFNWLEGDEIRERDWAF
                     FNRCYRQTYREHRSSPYLNLEFFCRIGRTLPRHTVMIVAERGGTPIAVSLNIRDSNRL
                     CGRYWGAMEYHPALHFETCYYQGIEYCIARGIATFEGGSRGEHKLARGLLPVETCSAH
                     WLAHPEFASAIEQFLAREACGMERYVDELNERGPFKRAPAAPSDRGPRQSP"
     gene            1304..2995
                     /locus_tag="AC16CX_09045"
     CDS             1304..2995
                     /locus_tag="AC16CX_09045"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009973075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3012694.1"
                     /translation="MKIAIAQINATVGDLSGNVARIADFAERARREGANLMVTPELAL
                     CGYPPEDLLLREDFLEACERALEDLAARAPAITLVVGHPRVTGGKRYNSASIIQNGKI
                     VSAYDKQRLPNYTVFDEERYFEPGTGPCVCAVNGVNFGVNICEDAWGTQGPITARAGD
                     AGRDARIGINICADSWNAEAPMAARQAGATVLLVLNASPYHVGKQRVRQDVMRERVAE
                     TGLAMVYVNLVGGQDELVFDGGSFVLNSAGVLTHQLPLFEEALALVTLTDGDPQPGEI
                     AAPLSPEAEIYRALQLGVRDYVNKNGFPGVLLGLSGGIDSALTLAVAVDALGAGRVRA
                     VMMPSQYTADISVEDARTEAKALGVRYAEIPIGPVFDAFIKALSAEFKGLPEDATEEN
                     IQSRIRGTLLMALSNKTGAIVLTTGNKSEMGTGYATLYGDMAGGFGVLKDINKMMVYR
                     LANYRNGISTVIPRRVIERAPSAELRANQTDQDSLPPYPVLDAIMEAYVESDLSPKEI
                     VAQGFGRSDVERVVRMVRTSEYKRRQAPVGIRITPRGFGKDWRFPITNKFRHQFD"
     gene            3131..3469
                     /locus_tag="AC16CX_09050"
     CDS             3131..3469
                     /locus_tag="AC16CX_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3012695.1"
                     /translation="MKKIEAIFKPFKLDEVREALSEIGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVIPDKLQESAIDAIVKAARTGKIGDGKIFVTSVEQVIRI
                     RTGETDEAAI"
     gene            complement(3528..4100)
                     /locus_tag="AC16CX_09055"
     CDS             complement(3528..4100)
                     /locus_tag="AC16CX_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR3012696.1"
                     /translation="MKKSRKPGIHPDEATLFRASMSDVTPLPRPGRVELERPLPRPIA
                     AQRLRDNRETLKDSLSDQLPWDAGMESGDELSYVRNGIAAQTLRKLRRGYWVMQEELD
                     LHGLTVDEARGLLVGFLNQCLRRGLRCVRIIHGKGLRSKNREPVLKRKVAGWLMQRDE
                     ILAFCQARRADGGSGAVVVLLKGESRKAEG"
     gene            complement(4144..5106)
                     /gene="trxB"
                     /locus_tag="AC16CX_09060"
     CDS             complement(4144..5106)
                     /gene="trxB"
                     /locus_tag="AC16CX_09060"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3012697.1"
                     /translation="MPSLPKHCRLLILGSGPAGYTAAVYAARANLKPVLITGLAQGGQ
                     LMTTTDVDNWPADAMGVQGPELMERFLKHAERFGTEIIFDHIHTARLKENPFVLIGDQ
                     GTYTADTLVIATGASAMYLGLPSEQKFMGRGVSGCATCDGFFYKGEEVAVVGGGNTAV
                     EEAIYLSNIASHVTVVHRRDRFRAEAILVDKLKDKVTAGKADILWDHVLDEVVGDNTG
                     VTGMRVKNVKTGATTGKSLKGVFIAIGHRPNTEIFEGQLDMTNGYIITRGGLDGNATA
                     TSVPGVFAAGDVQDHVYRQAVTSAGSGCMAALDAQKYLESRGLT"
     gene            5330..>6738
                     /locus_tag="AC16CX_09065"
     CDS             5330..>6738
                     /locus_tag="AC16CX_09065"
                     /inference="COORDINATES: protein motif:HMM:NF020068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR3012698.1"
                     /translation="MVSAAFADSSGLRDDSRSTGMDVYGYAIEGRVLLNAENFTRVIA
                     PFVGRHKTAADIERTRDALQRAYHDLGHCSVKVMLARPEPQDGVVRFRLVEVPAGEIR
                     ECLPVPTAQTPVGAETKIQVAAANPKSLETLPGVMVSPNVEGGGRELVLSGDGLTPGK
                     TPVAILAASDRATTSSAPAAQSSAVIPATLPTTPAPESKPVAPVPNTVLAQSEPARPA
                     PNGPAKETPAPVTKPATPPADLLPLAPIAAPKFEILRYLAEGNTLLTQEKLDQVLAPF
                     TGKNKDFGDVQRALEALQAAYQAAGYGGVEVRLPEQELERGEVRFTVIEVKIGKITVE
                     GNQHFSSHNIRRSVPSLREGVTPNSRQIAESIRLANESPAKQSAVLLRGSEREDQIDA
                     VIRVTDIDPKRSSASFDNTGTQNTGRTRLGIAYQHANVFDRDHVFTAQFITSPTKYQD
                     VNVVGAGYKIPFYSLGDSI"
BASE COUNT         1286 a         2056 c         2179 g         1217 t
ORIGIN      
        1 gctgaacagc gttgttatgg ctggttgttt tgtttcgagt gtacccgggc ggaaagaaat
       61 aagcgacagt aatccgtgtc atgggctttg acgcgggccc cgatcggaag gcgctgcggg
      121 cgcgcgcttg aacggcccgc gctcgttgag ctcgtcaacg tagcgttcca tgccgcaggc
      181 ctcacgcgcg agaaactgct cgatggcgga ggcgaattcc gggtgcgcca gccagtgtgc
      241 cgagcaggtc tcgaccggca gcaggccgcg cgcgagcttg tgctcgcccc gcgagccgcc
      301 ttcgaaggtc gcgatgccgc gcgcgatgca gtattcaatg ccctggtagt agcaggtctc
      361 gaagtgcagt gcgggatgat attccatggc gccccagtaa cgtccgcata gcctgttcga
      421 atcgcggatg ttgagcgaaa cggcgatggg ggtgccgccg cgctccgcga cgatcatcac
      481 ggtatgccgc ggcagggtgc ggccgatgcg gcagaagaac tcgaggttga ggtaagggct
      541 ggaacgatgc tcgcggtagg tctggcggta gcaacggttg aaaaacgccc aatcgcgctc
      601 ccggatctcg tcgccttcca gccagttgaa gtgaatgccg gtattgctca ccttgcggcg
      661 ttcctgcctc accctcttgc gcttgtcgtg gctgaacccg gcaaggaatt catcaaagct
      721 gccgtagccg cgattggtcc agtgaaactg cacggcatga cgcggcatca tgccgtgcgc
      781 gcgcatccgc tgcgcttcgc cgcggagggg aaacaggcaa tgcagcgagg acacccgcag
      841 cttgcgcgcg agctcgagcg ccgccgagat cagacggtcg cgatcctcgt ccgtcgtggc
      901 gagcaggcgc gtgccggtga cgggagtgaa tggcacggcc gagaccagct tgggatagta
      961 ggcgactccg tggcggtaat aagcgtccgc ccacgcccag tcgaaaacat actcgccgta
     1021 ggaatgggcc ttgagataga gcggcatcgc tcccgccagg aggccgttcc gtttcaacag
     1081 gaggtattgt ggcgcccatc cgctcgcgct gctcgcgcag cccgtctcat gcagcgccga
     1141 caggaactcg tggctcagaa acgggtcgcc gcctgccagg cggttccact cgccagcggc
     1201 ggtgccggaa agcgattcga cgaccttgat cgtcattcgc gggtgcgtgt ccgagactgc
     1261 gcgagctcgc gcgtgcaaac cggtcggatg atatattagc gacatgaaaa ttgcgatcgc
     1321 gcagatcaac gccacggtcg gcgacttgag cggcaatgtc gcgcgcatcg ccgacttcgc
     1381 cgagcgcgcg cgccgggaag gcgcgaatct catggtgacg ccggaacttg cgctctgcgg
     1441 ttatcccccc gaggacctgc tgctgcgcga ggatttcctg gaagcctgcg agcgcgccct
     1501 tgaagacctg gccgcgcggg cgccggcgat caccctggtc gtcggacatc cgcgcgtgac
     1561 gggcggcaag cgctacaact cggcgtcgat catccagaac ggaaagatcg tcagtgccta
     1621 cgacaagcag cgcctgccga attacacggt attcgacgaa gaacgctact tcgaaccggg
     1681 aaccgggccg tgcgtgtgcg cggtgaacgg ggtgaacttc ggggtcaaca tctgcgagga
     1741 tgcctggggt acccaggggc cgatcaccgc gcgggccggg gacgccgggc gggacgcccg
     1801 catcggcatc aacatctgcg ccgactcctg gaacgcggag gcgccgatgg cggcgcggca
     1861 ggcgggcgcc accgtgttgc tcgtgctcaa cgcctcgccc taccatgtcg gcaagcagcg
     1921 cgtgcggcag gacgtgatgc gcgagcgggt agcggagacc ggattggcga tggtctacgt
     1981 caacctcgtc ggcgggcagg atgagctggt gttcgacggc ggttccttcg tgctcaacag
     2041 cgcaggcgtc ctcacgcacc agcttccgct gttcgaggaa gcgttggcgc tggtcacgct
     2101 caccgatggc gatccgcagc ccggcgaaat cgccgcgccg ctttcgcccg aggccgagat
     2161 ttatcgggcg ctgcagctcg gcgtgcgcga ctatgtcaat aaaaacggct ttcccggcgt
     2221 gctgctcgga ttatcgggcg ggatcgactc cgcgctcacg ctggcggtcg cagtggacgc
     2281 gctgggcgcc gggcgcgtcc gcgcggtgat gatgccttcg cagtacaccg ccgatatcag
     2341 cgtggaggat gcgcgcaccg aggcgaaggc gctcggcgtg cgctacgccg agattccgat
     2401 cgggccggtc ttcgatgcgt ttatcaaggc gctttcggcg gagttcaaag ggctgccgga
     2461 ggacgcgacg gaggagaaca tccaatcgcg gatccgcggc acgctgctga tggcgctctc
     2521 caacaagacg ggtgcgatcg tgctcaccac cggcaacaag agcgagatgg gcacgggata
     2581 cgcgaccctg tacggagaca tggcgggagg cttcggtgtg ctgaaggata tcaacaagat
     2641 gatggtctac cggctcgcga actaccggaa cggcatttcc acagtgatcc cccggcgcgt
     2701 gatcgagcgc gcgccgtcgg ccgagctgcg cgcgaaccag accgaccagg acagcctgcc
     2761 gccctacccg gtgctcgatg ccatcatgga ggcgtatgtc gagagcgatc tcagccccaa
     2821 ggagatcgtc gcccaaggct ttggcaggag cgatgtcgag cgggtggtcc ggatggtgcg
     2881 gaccagcgaa tacaaacggc ggcaggctcc cgtcgggatc cgcatcaccc cgcgcggttt
     2941 cggcaaggac tggcggtttc ccatcaccaa caagttccgg caccagttcg attagcggcc
     3001 tgtgatatcc ttaagaggcc gtttcatagt gactgaaacg gcccctgatg gcggggagca
     3061 tgaggggagt gacccctcat atcgttaccg tctctaaggc aaacgcggga ccggatagtg
     3121 gaggggcctc atgaagaaaa tcgaagcgat cttcaaacct ttcaagcttg acgaagtgcg
     3181 cgaggcattg tccgagatcg gcgtgagcgg cctgaccgtc acggaagtga aaggcttcgg
     3241 caggcagaaa ggccataccg agctctaccg cggggccgag tacgtcgtgg acttcctgcc
     3301 caaggtcaag gttgaggtgg tgattcccga caaactgcag gagagcgcga tcgacgccat
     3361 cgtcaaggcg gcacgcaccg gcaagatcgg ggacggcaag atttttgtca ccagcgtcga
     3421 acaggtcatc cgcatccgca ccggcgaaac cgacgaggcg gccatctaaa ggcggaaggc
     3481 ggaaggagaa agggaacgga ggggagaaca gcaaaacggc tcttacttca tccttccgcc
     3541 tttcgacttt cgcctttcag cagcaccacc accgcgccgc tgccgccgtc agcgcgccgc
     3601 gcttggcaga acgccaggat ttcatcgcgc tgcatcagcc agccggccac cttgcgcttg
     3661 agcaccggtt cgcggttttt tgagcgcaac cccttgccgt gaatgatgcg gacgcagcgc
     3721 aacccccggc gtaagcattg gttgaggaac cccaccagca ggccgcgagc ctcgtcgacg
     3781 gtcaggccgt gcaggtccag ttcttcctgc atgacccagt acccgcggcg caatttcctg
     3841 agagtttgcg cggcgatgcc gttgcgcaca taggaaagct cgtcgccgct ttccatgccg
     3901 gcgtcccacg gcagctgatc gctgagactg tccttcagcg tttcccggtt gtcgcgcagg
     3961 cgctgtgccg caatcgggcg cggaagcggt cgttcgagct cgaccctccc cgggcgcggc
     4021 aagggcgtca catccgacat cgatgcgcgg aacagcgtcg cctcgtcggg atggatgcca
     4081 ggtttacggc tctttttcat cgctctacgc gcggctggcc tgcggaccca gcggctgctg
     4141 gacttacgtc agcccgcggc tttccagata tttttgcgca tccagcgccg ccatgcagcc
     4201 gctgccggcg ctggtgaccg cctggcgata gacgtgatcc tgcacatctc cggccgcgaa
     4261 cacgcccggc acactggtgg cggtggcgtt tccgtccagg ccgccccggg tgatgatgta
     4321 gccgtttgtc atgtcgagct gtccttcgaa gatctccgtg ttgggacgat gtccgatggc
     4381 aatgaatacc cccttcaatg atttgcccgt ggtcgcgccg gtcttgacgt tcttcacacg
     4441 catgccggtg acgccggtat tgtcgcccac gacctcgtcg agtacgtggt cccacaggat
     4501 gtcggccttg cccgcggtga ctttatcctt cagtttgtcc acgagtatgg cctccgcgcg
     4561 gaatctgtcc cggcggtgca ccacggtcac gtgactggcg atgttggaca ggtagatcgc
     4621 ttcctcgacc gcagtgttgc cgcctcccac cacggcgact tcctcgccct tgtagaaaaa
     4681 cccgtcacaa gtggcgcatc ccgagacgcc gcgccccatg aatttctgct cggaggggag
     4741 accgaggtac atcgctgaag cgccggtggc gatgacgagg gtatcggcgg tgtaggtccc
     4801 ctggtcgccg atcagaacga aggggttttc cttgagtctg gcggtgtgga tgtgatcgaa
     4861 gatgatctcc gtaccaaagc gctcggcatg cttgaggaag cgctccatta gttccggtcc
     4921 ctgcacgccc atcgcgtcgg ccggccagtt gtcgacatcg gtggtggtca tcaattgccc
     4981 gccttgcgca aggccggtga tcagaaccgg cttcaagttg gcacgcgcgg cgtaaacagc
     5041 ggcggtatag cctgcggggc cggatccaag aatgagcagg cgacagtgtt tgggaagaga
     5101 gggcatgagg ctagaggatg gggattattt gtaaaaaagc aacactttag cagcgggagt
     5161 tctaaagtgt cgagtttcag cagtacatga actgtttggc tttaagtcct tgctataaaa
     5221 tgcaaaatta ggtcgttgcg cgtcgcagtc gctgcacggc tgtcgaaacc aagggaggct
     5281 gatgaggatt gcagtaaggg gagggtgggc tggacttgct ctcgtggcga tggtgtcggc
     5341 cgccttcgcc gattcttcgg gtttgcgcga tgattcccgc agcaccggga tggacgtata
     5401 cggctacgcg atcgaaggcc gtgtcctcct caatgcggaa aatttcaccc gcgtcattgc
     5461 cccgtttgtt ggacgccata agactgcagc cgacattgaa cgcacgcggg acgcgctgca
     5521 gcgcgcctac cacgatctcg ggcattgttc ggtcaaagtc atgttggcac ggcccgagcc
     5581 acaggacggc gtggtgaggt tccgcctggt cgaggtgccg gccggcgaaa tccgcgaatg
     5641 cctgccggta ccgacagcgc aaacgcctgt aggagcagaa acgaaaatac aggtcgcggc
     5701 cgccaatcca aagagccttg aaacgctgcc gggcgttatg gtttcgccca atgtcgaggg
     5761 cggtggccgg gaattggtct tatcgggtga tggcttgacg ccgggaaaaa cgcccgtagc
     5821 aatcctcgcc gccagcgatc gcgctaccac gtccagtgcg cccgctgctc agtcatccgc
     5881 ggtgatcccc gctaccctgc caacaacccc cgcgccagaa tcaaagcccg ttgcaccggt
     5941 gccgaatacc gtccttgctc agtccgagcc cgcaaggcct gcgcccaatg gcccggccaa
     6001 ggaaacgccc gcacccgtca cgaagcctgc aactccgccc gccgatttgc tgccgcttgc
     6061 tccgattgcc gctcccaagt tcgagattct gcgttacctg gccgaaggca atacgttgct
     6121 cacgcaggaa aagctcgacc aggtgctcgc ccccttcacc ggcaagaaca aggatttcgg
     6181 ggacgtgcag cgggcgttgg aagcgctcca ggctgcttac caggccgccg gatacggcgg
     6241 agtggaggtc cgcttgcccg agcaggaact cgagcgcggc gaggtcagat tcacggtgat
     6301 cgaggtcaag atcggcaaga tcacggtcga gggcaaccag catttcagca gccacaacat
     6361 taggcgcagc gtaccgtcac tgcgcgaggg cgtaacgccg aattcccggc aaatcgctga
     6421 aagcatcagg ctcgccaacg aaagcccggc caagcagtcg gccgtgttgt tgcggggatc
     6481 ggagcgggaa gatcagattg atgcggtaat ccgggtcacc gacatcgacc ctaaacggtc
     6541 gagcgcaagt tttgacaata ccggtaccca gaataccggc cgcacccgcc tggggattgc
     6601 ctaccagcac gcgaacgtct tcgatcggga tcacgtcttc accgcgcaat tcatcacctc
     6661 gccaacgaaa taccaggacg tcaatgttgt cggcgctgga tacaagatac cgttttattc
     6721 gctcggcgac tcgatcga
//
LOCUS       DBNGWZ010000311         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_788767, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000311 DBNGWZ010000000
VERSION     DBNGWZ010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_788767"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="AC16CX_09070"
     CDS             complement(<1..1144)
                     /locus_tag="AC16CX_09070"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HIR3012699.1"
                     /translation="MPDDSKRKHRASLLKGARPRLDFQEHLADLEAEGLLVRVERPIN
                     KDTELHPLVRWQFLGGIPEDKRRAFLFTNVVDAKGRRYDMPVAVGALASSPQIYAIGM
                     GKSVEEIGPTWMRAIANPIAPVAVRSPACQQVVITGDALRKPGGGLARLPVPISTPGF
                     DAAPYLTATLCVTRDPENGIQNMGTYRAGLKATDRLGVRMVARVGGAGGYLHWLKYRS
                     RKAPMPIAIVIGCAPVVFFTGPQKLAVDRDELEVAGGLAGGPIRKTKCVTVDLDVPAD
                     AEIVIEGVIDTAVLEPEAPFGESNGYVALEAYNMPMQVTAITHKRSPVFTSIISQVTP
                     SESSLVKKVAYEPLFLAHLRDSLGIKSVRRVVMHEPLSNLRPVIFVQ"
     gene            complement(1163..2116)
                     /locus_tag="AC16CX_09075"
     CDS             complement(1163..2116)
                     /locus_tag="AC16CX_09075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3012700.1"
                     /translation="MLPSRVAAWVFFLGVHLLGADVAIGQDKYPGKLIRIVTATPGSN
                     HDWGARLTAQELTPRLGQRVIVENRGTIAIEYVAKEAQPDGYTLLFYGATVWLQPYLT
                     KASWDPVGDFAPITLAMSSPNVLVVHPSLPARSVRELIALARARPGELNYSAGGGGST
                     PHIAAELFNYMANVRIVRVHYKGSGPSMIGLLVGEVQLMFAALGPISPHVKQGKVRAL
                     AVSTPKRTPLMPELPAVSETLPGYTAEATIGFFAPRKTSPAIVTFLNREIVHALKSAD
                     PKVLFNAGIEIVGGSPEEFAGFIKAEMDRMGKVIKSASFNN"
     gene            2346..>3028
                     /locus_tag="AC16CX_09080"
     CDS             2346..>3028
                     /locus_tag="AC16CX_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012701.1"
                     /translation="MLAVACATASAQPYPSRPIRLVVPTTPGGSVDTLARTIGPKLSE
                     RWGQQVVVDNRAGAGGTIAAEFTAKSPPDGYTVMLGTIASLGTNVSLQKKLPYDPLKD
                     FSPVTLVATQNLVLTVHPSLPAKNVRELVKFAKSRPGQLTFASAGNGTGSHLSGELFK
                     LLAGLDLLHIPYKGVAPALVDVISGQVSMSFPSILTSLAQVHSGRLRALAVTGAQRSS
                     ALPDLPTMQ"
BASE COUNT          526 a          992 c          998 g          512 t
ORIGIN      
        1 actgcacgaa tatcacaggg cgcaggttcg acaacggctc gtgcatcacc acgcggcgca
       61 cgctcttgat tcccaggctg tcgcgcagat gcgcgaggaa cagcggctcg tacgcaacct
      121 tcttcaccag gctcgattcg ctcggcgtga cctggctgat gatggacgtg aacaccggag
      181 agcgcttgtg cgtgatcgcc gtgacctgca tcggcatgtt gtaggcttcg agcgccacgt
      241 agccgttgct ctcgccgaac ggcgcctccg gctcgagcac cgccgtatcg atcacgcctt
      301 cgatgacgat ttccgcgtcc gccggcacgt cgagatcgac ggtgacgcat ttcgtcttgc
      361 gtatcggccc gccggcaagc ccgccggcga cttccagctc gtcgcggtcg acggcgagct
      421 tctgcgggcc ggtgaagaac accaccggcg cgcagccgat gacgatggcg atcggcatgg
      481 gcgccttgcg gctgcggtac ttgagccagt gcaggtaacc gccggcgccg ccaacccgcg
      541 cgaccatgcg cacgccgagc cggtcggtgg ccttgagccc ggcgcggtag gtgcccatgt
      601 tctgtatgcc gttctccgga tcgcgcgtga cgcacagcgt cgcggtgaga tagggcgcgg
      661 cgtcgaagcc cggcgtcgag atcggcacgg gcaggcgcgc aagcccgccg ccgggcttgc
      721 gcagcgcgtc gccggtgatc acgacctgct ggcacgcggg cgagcggacg gcgaccgggg
      781 caatcgggtt cgcgatggca cgcatccagg tcgggccgat ttcctcgacc gacttgccca
      841 tgccgatcgc gtagatctgc ggcgaggacg cgagcgcgcc caccgccacc ggcatgtcgt
      901 agcgccggcc cttggcgtcg acgacgttgg tgaacaggaa cgcgcggcgc ttgtcctcgg
      961 gaatgccgcc caggaactgc cagcgcacca gcggatgcag ctcggtgtcc ttgttgatcg
     1021 gccgctcgac gcgcaccagc aggccctccg cctcgaggtc ggcaagatgc tcctggaaat
     1081 caagccgtgg ccgcgcgccc ttcagcaaag acgcgcggtg ttttcgcttc gaatcgtccg
     1141 gcatatcgac agccctccgg aatcaattgt tgaaggaagc gctcttgatg accttgccca
     1201 tccggtccat ttcggccttg atgaagcccg cgaactcctc gggggaaccg ccgacgattt
     1261 cgatcccggc gttgaacagc accttgggat ccgccgactt gagcgcgtgg acgatctcgc
     1321 ggttgaggaa cgtgacgatg gccggggacg tcttgcgcgg agcgaaaaac ccgatggtcg
     1381 cctcggccgt gtagcccggc agggtttccg agacggcggg caactcgggc atgagcggcg
     1441 ttcgtttggg cgtcgaaacc gccagcgccc ttaccttgcc ctgtttgacg tggggcgata
     1501 tcggtcccag ggcggcgaac atcaactgca cttcgcccac cagcagcccg atcatcgacg
     1561 ggccggagcc cttgtaatgg acgcgaacga ttcggacgtt cgccatgtag ttgaacagct
     1621 ccgccgcgat gtgcggtgtc gatccgccgc ctcctgcgct gtagttgagc tccccgggcc
     1681 gggctctggc gagcgcaatc agttccctga cggacctggc cggcaacgag ggatgcacca
     1741 cgagtacgtt gggcgagctc atcgccaggg taatcggcgc gaaatctcct accggatccc
     1801 agctcgcctt cgtgaggtag ggctgcagcc agacggtcgc gccgtaaaac agcaacgtgt
     1861 atccgtccgg ctgtgcctcc ttcgccacgt actcgatcgc gatggtgccg cggttctcca
     1921 cgatcacccg ctggccgagg cgcggcgtaa gctcctgcgc ggtgaggcgc gcaccccaat
     1981 cgtggttgct gccgggcgtc gccgtgacga tgcggatgag cttgcccgga tacttgtcct
     2041 ggccaatcgc cacgtccgcg ccaagcaggt gcacaccgag gaagaacacc caggctgcga
     2101 cacgggaagg caacatatct ccacctcgga ttgaggaatt cacctgcgcc ggcgataggg
     2161 cctatcataa cgccagaacg tggtgacgaa gtgaagcagt gacgcggtga cgagcaaaag
     2221 cggagggagg agggatgaag aacaaggcga aaggcgaaag gcgaaagtcg aaaggaaaag
     2281 accaagggcc taagggccgt tacttccgcc tttcgccttt cgccattcgc cttggatcgg
     2341 ctgccgtgct ggcggtggcc tgcgccacgg ccagcgcgca gccgtatcct tcccggccga
     2401 tccggttggt cgtgccgacc acgccgggcg gaagcgtcga tacgctcgcc cgcaccatcg
     2461 ggcccaaatt gagcgagcgc tggggccagc aggtcgtcgt ggacaaccgc gcgggtgcgg
     2521 gcggcacgat cgccgcggaa ttcaccgcca agtcaccgcc ggacggttac acggtgatgc
     2581 tcggcaccat tgcgtcgctt ggcaccaatg tcagcctgca gaagaagctg ccgtacgatc
     2641 cgctcaagga tttctcgccg gtgacgctgg tggcgacaca gaacctcgtg ctgacggtcc
     2701 atccctcgct ccccgcgaaa aacgtgcgcg agctggtaaa gttcgcgaag tcccgtcccg
     2761 ggcagctcac gttcgcatcg gcgggcaatg gcaccggcag ccacctctcc ggggagctgt
     2821 tcaagctgct cgccggcctc gatctgcttc acatcccgta caagggcgtc gcgccggcgc
     2881 tggtggacgt gatcagcggg caggtgtcga tgagctttcc gagcatcctg acatcgttgg
     2941 cgcaggtgca ttcgggaagg ctccgcgcac tggcagtcac cggggcgcaa cgctcttctg
     3001 ccctgcccga cctgccgacg atgcagga
//
LOCUS       DBNGWZ010000312         3988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1797783, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000312 DBNGWZ010000000
VERSION     DBNGWZ010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1797783"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="AC16CX_09085"
     CDS             complement(<1..261)
                     /locus_tag="AC16CX_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3012702.1"
                     /translation="MPRSLGTDNMDAKEPASLADDPARPPLRAPSPGRPIRNIAIIAH
                     VDHGKTTLVDQLLRQSGTFAAHQHLVERVMDSNDLERERGITI"
     gene            530..1390
                     /locus_tag="AC16CX_09090"
     CDS             530..1390
                     /locus_tag="AC16CX_09090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3012703.1"
                     /translation="MTPFELLEPRSLREAAGLLDTDEPSVRPIAGGTAVMLMMKIGVL
                     RPARLVSLRAVERRYSEISTGPDGGLRIGAMTTLSALERSPAVRNKAPVITRTLKTLS
                     NVRVRNVATVGGHLAHADPHMDLPPVLVALGASVSIVSPSGDRILPLEKLYAGYLETT
                     LGRNELIAEVTVPTQLPRRAAYLKCTTRSADDWPALGVAVVLEMDGAVVRNARIVISA
                     ATDMPTRLAAAENALRGARVDDAMLRRAGEAAAAEAGVIGDAHGSAAYKRELLRVYLG
                     RAVRQALAQK"
     gene            1504..3792
                     /locus_tag="AC16CX_09095"
     CDS             1504..3792
                     /locus_tag="AC16CX_09095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012704.1"
                     /translation="MTTKTAQSGTVGRSVPRLEGRAKVTGRAEYVHNLHLPGMLYGKI
                     FRSTIAHGRILRIDTSAARALPGVHRVITGVDIRAVIPEPYYGPAFHDQPILALDKVR
                     YVGEPVAVVLASDPHVAEEAAHQIVAEYEPLPAVFDEVEAMTSKAIVHEVLKPAGTFP
                     DLKHLQGRKNTNVALDYHLRRGDAELAFARADRVFEHTFRTQQVLHVPLEPFVSVADP
                     AESSVTIHTASQSPSYVRIEVARLLGWAENRVRVKVPYLGGGFGAKLYIKLEALVAAL
                     ALLVRRPVKVSLTMEEQFYTLTKHASTLRIKSGVAKDGRIVARQCEVWWNGGAYADVG
                     PRVTQKSGFTAPGPYDIENVHIDSYALYTNLPPAGALRGFGIPQLVWAYESHTDLIAR
                     ELKMDPLDFRRKNILRDGRPQATGTVMKDAAIDKALERVAARMDWSKPFDRSGGTLRR
                     GRGIAIGFKASISPTTSVAIVNLYADGSCALYCSTVDMGQGSDTAMAQIVAEVLGIPI
                     ESITVVHPDTDVTPYDMATLGSRSTFHMGNAVRRAAEDARDKLLALAREVGVSAETTP
                     IAEVFRKKYGMQAGNIVGTGSFIPTYTPTDHDTGQSTNVTPFWMVGATGAEVEVDTET
                     GQVRVTRLVNVADVGTPINPRIVETQLSGAAIMQLGFTLFEKMNFEAGQVTNASLAEY
                     KIPGMLDIPATIANEAVDAEQHSGPFGAKGVGETGTFSVSPAIANAIHDAVGVRLTSL
                     PLTAEAVYRALREKQGNPLKDN"
BASE COUNT          708 a         1302 c         1322 g          656 t
ORIGIN      
        1 gatggtgatg ccgcgctcgc gctcgaggtc gttcgagtcc atcacgcgct cgaccaggtg
       61 ctggtgcgcg gcgaaggtgc cggactggcg cagcagctgg tccaccagcg tggtcttgcc
      121 gtggtcgacg tgcgcgatga tcgcgatgtt gcggatggga cgacccggac tcggcgcgcg
      181 cagcgggggg cgggcagggt cgtcggcgag cgacgcgggt tctttcgcat ccatgttatc
      241 ggttccgagc gagcgcggca tgaggcggtg ttctctacgc aaaagcgcgc gattttagca
      301 tataaacgac tgttttcttg aattttcttt accttgtcag taaagttctg catcgcctgg
      361 cgtccaaccg gaatcgtccc gtgcctttat tcgatctgtg tttatccgcg ttcaaccctc
      421 acccccgccc ctctcccgag cggagagggg aggatcgagc ttcccttctc cttggggaga
      481 aggggaaggg atgagggatg tgcggttccg acagctttag gactcaggca tgacgccgtt
      541 cgagctgctg gagccgcgct cgctgcgcga ggcggccggc cttctggaca ccgacgagcc
      601 ctccgtccgc cccatcgcgg gaggcaccgc ggtgatgctg atgatgaaga taggcgtgct
      661 gcgtccggcc cggctcgtca gcctgcgcgc ggtcgagcgg cgctactccg agattagcac
      721 cggtcccgac ggcgggcttc gcatcggcgc gatgacgacc ttgtcggcgc tggagcgttc
      781 gcccgcggtc aggaacaagg cgccggtcat caccaggaca ctgaagacgc tgtccaacgt
      841 gcgcgtgcgc aatgtcgcga cggtcggtgg tcacctcgcg catgccgacc cgcacatgga
      901 tttgccgccg gtgctggtcg cgctcggagc gagcgtttcc atcgtgagtc cttccggcga
      961 tcgcatcctg ccgctcgaga agctgtatgc cggctatctc gaaacgacgc tcggccgcaa
     1021 cgagctcatc gcagaggtga ctgtcccgac gcagcttccc cgccgcgccg cctatctcaa
     1081 gtgcaccacg cggtcggcgg acgactggcc ggcgctcggc gtggcagtcg tgctggagat
     1141 ggacggtgcc gtcgtgcgca acgcgcgcat cgtgatcagc gccgcgaccg acatgcccac
     1201 gcgccttgcc gcggcggaga acgcgctgcg cggcgcgcgc gtggatgacg ccatgctgcg
     1261 tcgggcggga gaggcggctg ccgcggaagc cggcgtaata ggagacgcgc acggttcggc
     1321 ggcgtacaag cgcgagctcc ttcgcgtata tctcgggcgg gcggtaagac aggcgttagc
     1381 gcaaaaatga cgacaacgga tcgggagagc gggagagcgg gaattcggac tgcgttgctt
     1441 ttcgatcgct cggttttcgc cctcccgatc tcccgatttc acgacttccg tcttctggtg
     1501 gcaatgacga caaagacggc acaaagcggc acggtcggta gatcggtgcc tcggctggag
     1561 ggaagagcca aggtgaccgg ccgcgcggag tacgtccaca acctgcacct gccgggcatg
     1621 ctgtacggaa agatcttccg cagcacgatc gcacacggcc gcatcctgcg catcgacacg
     1681 agcgccgccc gggcgcttcc cggagtccat cgcgtcatca ccggcgtgga catccgcgcc
     1741 gtcatccccg agccgtacta cggtcccgct ttccacgacc agccgatcct tgcgctcgac
     1801 aaggtgcgct acgtcggcga gccggtcgcg gtcgtgctgg cgtccgaccc gcacgtcgcc
     1861 gaggaggccg cgcatcagat cgtcgcggaa tacgagccgc tgcccgcggt attcgatgag
     1921 gtcgaggcga tgacctcgaa ggcgatcgtg cacgaggtgc tcaagccggc ggggacgttc
     1981 cccgatctca agcacctgca gggcaggaag aacaccaacg tcgcgctcga ctatcacctg
     2041 cggcgcggcg atgcggaatt ggcgttcgcc agggccgacc gcgtattcga gcataccttc
     2101 cgcacccagc aggtgctcca cgtgccgctc gagccgttcg tctcagtggc ggaccccgcg
     2161 gagtcatcgg tcacgatcca caccgcctcg caaagccctt cgtacgtgcg catcgaagtc
     2221 gcacgcctgc ttggctgggc agagaaccgg gtgcgcgtga aggttccgta cctcggcggg
     2281 ggcttcggcg cgaagctcta catcaagctc gaggcgctcg tcgccgcgct cgcgcttctc
     2341 gtccgccgcc cggtcaaggt ctcgctcacg atggaggagc agttctacac gctcaccaag
     2401 cacgcgtcca cgctccgcat caagagcggc gtggcgaagg acggacgcat cgtcgcgcgg
     2461 cagtgcgaag tgtggtggaa cggcggcgcc tacgccgacg tcggcccgcg cgtgacgcag
     2521 aagtcgggct tcaccgcgcc ggggccgtac gacatcgaga acgtgcacat cgattcgtac
     2581 gcgctctata ccaatctgcc gccggccgga gcgctgcgcg gtttcggcat cccgcagctc
     2641 gtgtgggcgt acgaaagcca caccgacctg atcgcgcgcg agctgaagat ggatccgctc
     2701 gatttccgcc ggaagaacat cctgcgcgac ggccgcccgc aggcgaccgg cacggtgatg
     2761 aaggacgcgg cgatcgacaa ggcgctcgag cgggtggcgg cgcgcatgga ctggagcaag
     2821 cccttcgacc gcagcggcgg aacgctccgg cgcggccgcg gcatcgcgat cggcttcaag
     2881 gcgagcattt cgccgacgac ttcggtcgcc atcgtcaatc tctatgccga cggcagctgc
     2941 gccctctact gcagcaccgt ggacatgggg cagggctcgg acacggcgat ggcgcagatc
     3001 gtcgccgagg tgcttgggat cccgatcgaa tccatcaccg tggtgcaccc cgataccgac
     3061 gtgacgccct acgacatggc gacgctggga tcgcgctcga ccttccacat gggcaacgca
     3121 gtgagacgcg cggcggagga cgcgcgcgac aagctgctcg cgcttgcgcg ggaagtcggc
     3181 gtgtcggccg agacgacgcc gatcgcggaa gtattccgca agaagtacgg catgcaggcg
     3241 ggcaacatcg tcggcacggg cagcttcatt cccacttaca cgccgaccga tcacgacacg
     3301 gggcagtcca ccaacgtgac gccgttctgg atggtcggcg cgaccggcgc cgaggtcgag
     3361 gtggacaccg agaccgggca ggtgcgggtg acgcggctcg tcaacgtcgc cgacgtcggg
     3421 acaccgatca atccgaggat cgtcgagaca cagctttccg gcgccgcgat catgcagctc
     3481 ggcttcacgc tgttcgagaa aatgaatttc gaagccggcc aggtgaccaa cgcctcgcta
     3541 gccgaataca agatacccgg catgctcgac attccagcga ccatcgcgaa cgaggcggtg
     3601 gacgccgagc agcacagcgg accattcgga gcgaagggcg tcggcgagac cggcactttc
     3661 agcgtctcgc cggcgatcgc caacgcgatc cacgacgcgg ttggcgtgcg gctgacgtcc
     3721 ctgccgctga ccgccgaggc tgtttaccgc gcgctacgcg aaaagcaagg caacccgctg
     3781 aaagacaatt agccacagag agcacagaga acacagagaa agatgaactt ctcatccctc
     3841 atccctcatc cctcatccct tagcgggaac cgccgatgaa ctccgttcag cgcacaatcc
     3901 ggttcacgct gaacggcgag gcggtttccg cggacgttcc agctcattgg aacgtcgtgg
     3961 agctgctgca gaaccggttc ggtctcta
//
LOCUS       DBNGWZ010000313         5980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_663650, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000313 DBNGWZ010000000
VERSION     DBNGWZ010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_663650"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC16CX_09100"
     CDS             complement(<1..327)
                     /locus_tag="AC16CX_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012705.1"
                     /translation="MTVTATRERLFTAAYRRLLETGELARRVETAYRHLECCDLCARY
                     CRVNRGKGIEGAVCRTGERAVVASYGPHHGEEDPLRGRSGSGTIFFSWCNLRCAFCQN
                     WDISQSG"
     gene            complement(370..486)
                     /locus_tag="AC16CX_09105"
     CDS             complement(370..486)
                     /locus_tag="AC16CX_09105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3012706.1"
                     /translation="MVTLEGYVRTDEERRQAEADAWCLFAVDRVINRIEVRR"
     gene            complement(480..1139)
                     /locus_tag="AC16CX_09110"
     CDS             complement(480..1139)
                     /locus_tag="AC16CX_09110"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3012707.1"
                     /translation="MLPQRAPNNLMPREKQAMPGRNNVIKRVHGALEYERRINLHRYP
                     VKIGFVDGAVVLEGEVADVAAKKLALELAGAVDGVRGVIDRLHITPAERKGDGAIRDS
                     VRGAFLRQPEFRNCTIRSRAKGRVETLRDAPGGGEIEFVVEDGVVTLEGSVASLSHKR
                     IAGVLAWWAPGCRDVVNSLDVQPPEQDGDDEVVDALRLVLEMDPLVQAGQIARAAAIA
                     W"
     gene            1295..1615
                     /locus_tag="AC16CX_09115"
     CDS             1295..1615
                     /locus_tag="AC16CX_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012708.1"
                     /translation="MDWKADVIERVWERGRVMPEANPSVWRQDACGAWMRRDQFGRAR
                     AEFGWKIEKTAPGGPDTPDNLRPFHCRNRQDIASNRPHCAVTADRSGVGPGEFAGPPR
                     NRDI"
     gene            complement(1712..1891)
                     /locus_tag="AC16CX_09120"
     CDS             complement(1712..1891)
                     /locus_tag="AC16CX_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012709.1"
                     /translation="MFVIAPASASTWAVAFARRLIPGALFETKMAAISYALLLASSAG
                     LDSTSIKVLGDVQSR"
     gene            2101..2676
                     /locus_tag="AC16CX_09125"
     CDS             2101..2676
                     /locus_tag="AC16CX_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012710.1"
                     /translation="MAVELRLGLRRVMTLSLRGDSTNPGSVFRSMEKHPRLLRTEPIM
                     REILGVADEPRTRRRWFHNDYFDLFVWQTTSGEVTLFQLCYGIGSSERALVWHKYGAF
                     FHDGSNSRGSKEIIGAGFVPGKPLSADPVISRFESAAHSLPDDIRRVVTERIREYVEN
                     KPETPMRRKRFRRADWQKRPAASPTIQRGGA"
     gene            complement(2773..3738)
                     /locus_tag="AC16CX_09130"
     CDS             complement(2773..3738)
                     /locus_tag="AC16CX_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012711.1"
                     /translation="MPKLRCFALLCCFTSCAALAQQYPSRPVRVLIPFTAGSAADIIA
                     RAMEPAMRERLGQPFVIDNRGGAGGNIAAEMTAQATPDGHTIMMATIGTHAINHALYS
                     KLNFHPQRDFTPVGLVGESPNALVINPKVQANSIQDLIALAKSKPGRLNYGSSGAGTT
                     VHLSAELFSVMTGIKMTHVPHKGAAEALASLLGGQTDLMFASLSSSIPLIKAGRLRAF
                     AVTGAKRSPSIPELPTVAEAANLPGYEAAAWFGIVGPAGVPKNAVAVLNKAALSALDD
                     AEVKKRLFNAGVEIRTSSPQDFARYIESEMRKWAKVVKASGAKAD"
     gene            4014..5747
                     /locus_tag="AC16CX_09135"
     CDS             4014..5747
                     /locus_tag="AC16CX_09135"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4329..4331,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3012712.1"
                     /translation="MASHDGYYEALWPRSPRQTKVKPLAPRLETLAGKTVAQTWDYVF
                     RGDEVFEFLEQGLKARFPGIRFVGWREFGNTHGSEEREVVAALPKRLGELKVDAVISG
                     MGCUGSCTPAVLRASAAGEAAGVPSSTLVCEGFLPLAAAASVGLGMPNLPVARVIGHP
                     GVQSREQLEKNTLEVTLQQVIDNLLKAPKTAGSGREPGPREVIVKGSFEEVNEYFYKN
                     ELSDGLPIVPPTRDRVGQFLRYTDRDPDESLGSLLPDSRAATIWSIAVNGVMAGCRPE
                     YMPVLVALIEAMADPAYGVEHSGNTPGGETLILLNGPIIKELGFNYTQGVMRDGFMPN
                     TSIGRFWRLYLRNVAGFLPHKNDKATFGNTWRVVVAENEDVIKKIGWEPNSVDMGFAA
                     GDNTVTIARYTGGNHISSVSGSTPEQMMPYVCDAMVKQCSWQIMFTVGQGMGTLRPLM
                     LLSPIIAETIAGWGWSKRDLKRHVFDHARMPAHQFERILRDWTQKPIWNLAEEEKAGR
                     IPKVFHASDDPDRLVPLVWKPEDYMIAVTGDLTRNSVYIFAHNGVLGFPVAKRIRLPK
                     GWERLLTGEKA"
BASE COUNT         1057 a         1933 c         1943 g         1047 t
ORIGIN      
        1 gccgctctgg gagatgtccc agttctggca aaacgcgcag cgcaggttgc accagctgaa
       61 gaagatcgtc ccggacccgg accggccgcg cagcggatcc tcctcgccgt ggtgcggccc
      121 gtagctcgcg accaccgcgc gctcacccgt gcggcagacc gcgccttcga tgcccttccc
      181 gcggttcacg cggcagtagc gcgcgcacag gtcgcagcat tcgaggtggc gataggcggt
      241 ttcgacccgc cgcgcgagct cgccagtctc gagcagccgc cggtaggccg cggtgaagag
      301 ccgttcgcgt gtcgccgtga cggtcatgcc cggctccccc cgcgcggcgc tcttgcaagc
      361 ttcatgagat tatcggcgca cctcgatcct gttgatcacc ctgtccaccg cgaacaggca
      421 ccacgcatcg gcttccgcct gccgccgctc ttcgtcggtc cggacatagc cctcgagcgt
      481 caccacgcaa ttgcggcagc tcgcgcgatc tggccggcct gcaccagcgg gtccatctcc
      541 agcaccagcc gcaacgcgtc cacgacctcg tcgtcaccgt cctgttcggg gggttgcacg
      601 tccagcgagt tcaccacgtc gcggcaaccg ggcgcccacc aggcgagcac acccgcgatg
      661 cgcttgtgcg agaggctggc gacactgcct tcgagcgtga cgacgccgtc ctcgaccacg
      721 aactcgatct cgccgccgcc gggcgcatcg cgcagcgttt cgacccggcc cttggcgcgg
      781 gagcgtatcg tgcagttccg gaactccggt tgccgcagga aggcgccgcg gaccgaatcg
      841 cggatcgcgc cgtcgccctt gcgctcggcc ggcgtgatgt gaagccggtc gatcacgccg
      901 cgcacgccgt ccaccgcgcc cgccagctcg agggcgagct ttttcgcggc aacgtccgcg
      961 acttccccct ccaagaccac cgcgccgtcg acgaacccga tcttgaccgg gtagcggtga
     1021 aggttgatgc gccgctcgta ttcgagcgcc ccatgaaccc gcttgatcac gttgttcctg
     1081 cccggcatgg cctgcttttc ccgcggcatg aggttgttcg gcgcccgttg cggcagcacc
     1141 gctgcacaac aattgtgcgc cgccagccct cgcgcagttt gcgctaggtc aagaactgcc
     1201 gctgcggatc cctgtatttt gcagccgtgg ggtttccttc ctcaccgtcg cgggcagccg
     1261 gcgggattcc acggaatcgg ggtgtatcgg caccatggac tggaaagcgg acgtgatcga
     1321 gcgcgtctgg gagcgcggca gggtcatgcc cgaggcgaat ccgagcgtat ggcggcagga
     1381 cgcgtgcggg gcatggatgc ggcgcgacca gttcgggcgc gcgcgtgccg agttcggatg
     1441 gaagatcgaa aagaccgcgc ccggcgggcc ggatacgccc gacaaccttc gcccctttca
     1501 ctgccgcaac cgccaggaca tcgcgagcaa caggccgcat tgcgcggtga cggcggaccg
     1561 gtcgggcgta gggcccggag aattcgcggg cccgccgcgc aatcgcgaca tctagagagg
     1621 gccctccgct ttccgcgaac gtttatggcg tggacgccgc ggcggagaac ttcatgcgct
     1681 tcgagtagcg tgccccgccg ctcgtcgcgc ctcaccggct ttgcacgtcg cccaggacct
     1741 tgatgctcgt ggaatccagg ccagcgctgc ttgcgagcag aagcgcgtag ctgatcgccg
     1801 ccattttcgt ctcgaacagc gcccccggga tgagccgtcg tgcaaaggcg acagcccaag
     1861 tggaggcgct cgcaggtgca atgacaaaca ttcttttcct tctgtgctgc cagaggccct
     1921 tacgattcat ggatgtagtc ctcctctagc catgatggat gggaacgacc cggaaacggc
     1981 aaagaatcgt cccacccaag atgtcacgca agaaccagac caggataaca ggacctgaga
     2041 aaatttctta agcggccggg tcaggggcct gaatcaccgc gaccttttcc gatccggcgc
     2101 atggcggtcg agctccggct ggggctgcgc cgagtcatga cattgtcgtt gcgcggcgac
     2161 agtacgaacc ccggctctgt gttccggtcc atggagaaac atccccgtct tctccgcacc
     2221 gagcccatca tgcgtgaaat cctgggcgtg gccgatgaac cccggacgcg ccggcgctgg
     2281 ttccacaacg actacttcga tctattcgtg tggcagacca cgagcggcga agtcaccctg
     2341 ttccagctgt gctacggcat cggctcgagc gagcgggcgc tggtgtggca caagtacggt
     2401 gctttcttcc acgatggaag caactccagg ggctccaagg agatcatcgg tgcgggattc
     2461 gtcccgggca agccgttgag cgcggatccg gtcatctcgc gtttcgagtc agccgcgcac
     2521 tcgttgcctg acgacattcg ccgcgtcgtg acggaacgca ttcgcgagta cgtcgagaac
     2581 aagcccgaga ccccgatgcg ccgcaagcgc tttcgtcgcg cggactggca gaaacgaccc
     2641 gccgcgagtc caacgatcca gagaggcggg gcttagcctg ccattacgtc ggcgtaaggc
     2701 gtaaggcaga aggcagaagg cagaaggaga cgctgcctac tggttttcca cttcttcctt
     2761 catccttacg attcagtccg ccttggcgcc agacgccttc acgactttcg cccacttccg
     2821 catctcggat tcgatgtagc gcgcgaagtc ctgcggcgag ctggtccgga tctccacccc
     2881 ggcgttgaac agccgtttct tcacttccgc gtcgtcgagc gcggaaagcg ccgccttgtt
     2941 gagcacggcg acggcgttct tcggcacgcc ggccgggccg acgatgccga accaggcggc
     3001 ggcttcgtag cccggcaagt tcgccgcctc cgccacggtc ggcagctcgg ggatggaagg
     3061 cgaacgcttc gcgccggtga ccgcgaacgc cctgagcctt cccgccttga tgagcgggat
     3121 ggaagaggac aggctcgcga acatgaggtc ggtctgtccg ccgagcagcg aagccagcgc
     3181 ctcggccgcg cccttgtgcg gcacgtgggt catcttgatg ccggtcatca cgctgaagag
     3241 ttccgccgag agatgcacgg tcgtgccggc gccggacgat ccgtaattga gccgtcccgg
     3301 tttcgatttt gcaagcgcga tcaggtcctg gatcgagttc gcctgcacct tcgggtttat
     3361 caccagcgcg ttcgggctct cgcccaccag cccgaccggc gtgaaatcgc gttgaggatg
     3421 gaagttgagc ttggagtaaa gggcatggtt aatggcgtgc gtgccgatgg tggccatcat
     3481 gatggtgtgg ccgtcgggcg tcgcctgcgc cgtcatttcg gccgcgatgt tcccccccgc
     3541 cccgccgcgg ttgtcgatca cgaacggctg cccgagccgc tcgcgcatcg ccggctccat
     3601 cgcgcgggcg atgatgtcgg cggcgctgcc ggcggtgaac gggatcagaa cgcgcactgg
     3661 gcgcgacggg tactgctgcg ccagcgcggc acaggaggtg aagcagcaga gcaatgcgaa
     3721 acagcgtaac ttcggcatga catcctccct ggagggcgaa tttagcgcag cctagcacgg
     3781 ggcaataaac gtgataagtg ataagtgata taagtgacga gtgtcgggtg tcgggtgtcg
     3841 agggacgagt gacgagtgtc gggtgtcgag tggaaacgct cacttccctt aaccgttcta
     3901 gtttctcccc ttgacactca ttcctcgaca cttttcactc gacactccat ccctcatccc
     3961 tcggatgagg tatcctttca taatcgaatg aatatttcct gagagtccgg atcatggcga
     4021 gtcacgacgg atactacgaa gcgctgtggc cgcgcagccc gcggcagacc aaggtgaagc
     4081 cgctcgcgcc gcggctcgaa acgcttgcgg gcaagacggt cgcgcagacg tgggactacg
     4141 tgttccgcgg cgatgaagtg ttcgagttcc tcgagcaagg cctcaaagcg cgctttcccg
     4201 gcatccgctt cgtgggctgg cgcgagttcg gcaacacgca cggcagcgag gaacgcgagg
     4261 tggtggccgc gctgccgaag cgcctcgggg aattgaaggt cgacgccgtc atctccggca
     4321 tgggctgttg agggagctgc acgcccgccg tgttgcgggc gagcgcggcg ggcgaggcgg
     4381 cgggcgtgcc gtcctcgacg ctggtgtgcg agggctttct tcccctcgct gccgccgctt
     4441 cagtcgggct cggcatgccg aatctgccgg tggcgcgggt gatcggccac cccggcgtgc
     4501 agagcaggga acagctcgag aaaaacaccc tggaggtcac gctccagcag gtgatcgaca
     4561 atctcctcaa agcgcccaag acggcgggtt ccggccggga gccggggccg cgcgaggtaa
     4621 tcgtcaaggg cagcttcgag gaggtcaacg agtacttcta caagaacgag ctctccgacg
     4681 gcctgccgat cgttccgccg acgcgcgaca gggtcgggca gttcctgcgc tacaccgacc
     4741 gcgaccccga cgaatcgctc ggaagcctgc ttcccgacag ccgggccgcg acgatctgga
     4801 gcatcgcggt gaacggtgtg atggcgggat gccggcccga gtacatgccg gtgctggtcg
     4861 cgctgatcga ggccatggcc gatcccgcct acggcgtcga gcacagcggc aacacgccgg
     4921 gcggcgagac gctgatcctc ctcaacgggc cgatcatcaa ggagctcggc ttcaactata
     4981 cgcagggggt gatgcgcgac ggcttcatgc ccaacacctc gatcggccgg ttctggcggc
     5041 tgtacctgcg caacgtcgcc ggcttcctgc cgcacaagaa cgacaaggcc accttcggca
     5101 acacctggcg ggtcgtggtg gcggaaaacg aggacgtcat caagaagatc ggctgggagc
     5161 cgaacagcgt ggacatgggc ttcgccgcgg gcgacaacac cgtgaccatc gcgcgctaca
     5221 ccggcggcaa ccacatctcg tcggtctccg gcagcacacc cgagcagatg atgccctatg
     5281 tctgcgacgc catggtcaag caatgctcgt ggcagatcat gttcacggtg gggcagggca
     5341 tgggtacgct gcggccgctc atgctgctct cgccgatcat cgcggagacc attgcgggct
     5401 gggggtggtc gaagcgcgac ctcaagcggc acgttttcga tcatgcgcgc atgccggcgc
     5461 accagttcga gcgcatcctg cgcgactgga cccagaagcc gatctggaac ctggcggagg
     5521 aagagaaggc ggggcgaatt ccaaaagtct tccacgcctc ggacgacccg gaccgcctgg
     5581 tgccgctcgt gtggaagccc gaagactaca tgatcgcggt gacgggcgat ctcacgcgca
     5641 acagcgtcta tatcttcgcc cacaatgggg tattgggctt tccagtagcg aaacggatcc
     5701 ggctgcccaa gggttgggag cggctactta caggcgaaaa ggcgtgatga gtgacgagtg
     5761 ataagtgata ggactcgaac ggcgtgataa gtgataggtt tcccgcggat cggaggttgt
     5821 taatttcctg tcacctatca ccggggttta gctcctcatc cttcatcctt ctgccttcat
     5881 ccttcgggag gtccctcacc ccccgctgac ggcgcgggcc ggcggagggg aaaaacagcg
     5941 ttgtttgccg aatcggccgg cagggaagaa aatgaccttg
//
LOCUS       DBNGWZ010000314         4330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_789532, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000314 DBNGWZ010000000
VERSION     DBNGWZ010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_789532"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1018)
                     /locus_tag="AC16CX_09140"
     CDS             complement(17..1018)
                     /locus_tag="AC16CX_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012713.1"
                     /translation="MKACWKTFLGVSLCAAMTGGIHAAPARGSSTGSAPDFPSKPVRI
                     VIGFSPGGLPDIMARVIAQKLAESWTQPVVVENRPGAGGIVASQIVAGSAPDGYTMLA
                     VTSAHAATPALYAKLPFDAVRDFAGVTTMGTNAMLLVASSSLGVKSVKDLIALARARP
                     GQLNFSSAGVGSNTHFAMEIFKSMAGIDIVHVPYKGIPEALTETMAGRVQLFMAPLGL
                     ALPQVKDGKVQALGVTTTRRSSLLPDVPTVAESGLPAYRWVAWLGLLVPAKTPRAIID
                     RLNGEITRILSQPDVHQRWATLGVDVARSTPEQFDKIIRDEIVTFTKAARAANIKAE"
     gene            1172..2242
                     /locus_tag="AC16CX_09145"
     CDS             1172..2242
                     /locus_tag="AC16CX_09145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3012714.1"
                     /translation="MARDPQVDLIFSHFTPRYIGTGVDPNDLNRLMAGIDDWAEWCRI
                     WSDEAGRHERLATEAAERNRRVTAAEGFLRAAIYYHYAKHLFANSPEEYRTAHDNMLR
                     CYMAAAPGTDPPMERIEFPFEGGLMPGYLRRPRGVERPPVAIILPGLDACKEELHAWS
                     NAFLKRELATLTLDGPGQGETAYWLPIRSDWGRVIGAVIDVLEQRPDVNGYAVGIVGQ
                     SLGALYAPLAAAGESRIKACIANCGPFDWAALLPKMPKLSQEVFRARSHASTPARARE
                     IASQLTLEGAAERIRCPLLVVFGGGDRLVSPSEGERLARAASGPTEFVVYEEGNHVCF
                     NICYKFRPLTGDWMAERLAAKG"
     gene            complement(2377..3348)
                     /locus_tag="AC16CX_09150"
     CDS             complement(2377..3348)
                     /locus_tag="AC16CX_09150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012715.1"
                     /translation="MRPLRLAVCAGLLLGMFVSAGANSQNFPTRPLRYVMPLPAGGET
                     DLFGRVLAKQLADAWGQQVVVENRPGGGTTIATEFVAKAPADGHTILHAITPFGINAT
                     LYARLPYDTLKDFGCITHIGNLYGVLLAHPSFPAKNMAELVRLAKARPGEINYATGGS
                     GTANHVATEAIRLAAGINIVHVPYKGSSQAVLDVLPGRIPLLATVLVEAVPYIQSRKL
                     RVIATTSPKRAPSLPGVPTVGETLPNYQAGTGFWALVTRTGTPTAVLNKLNADIVKAL
                     HAPDVRARLAQADIELVGSRPEQCDAFLREQVGVWGKIVKASGARVD"
     gene            3473..>4330
                     /locus_tag="AC16CX_09155"
     CDS             3473..>4330
                     /locus_tag="AC16CX_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012716.1"
                     /translation="MPVLALGYIGVDATDLGAWRAFGTDVLAMQIVERGADALALRMD
                     ERCQRVLVQRAERDGPAFFGFETTGEQALKETVGKLEAAGVSMTRASEKELMLRRVDD
                     MAWCRDPAGNRVEFYHGQAAGNGPFMPPRPHGGFRTGRLGMGHMVLYAEDIDAMSAFY
                     RELLGLRLSDYMIKPYRLMFLRANPRHHSVGLLQSKHNGVHHVMFEVLALDDVGRAYD
                     FALEGWRIGQTLGRHANDWMLSFYAFCPAGFMMEYGWGGRTVDEASWTPREVTHGGSM
                     WGHDRLWLDE"
BASE COUNT          752 a         1348 c         1460 g          770 t
ORIGIN      
        1 gatcccgccc gacgcgctac tccgccttga tattggccgc gcgcgcagcc tttgtaaaag
       61 tgacaatctc gtcgcggatt attttgtcaa actgctccgg cgtactccgc gcaacatcta
      121 cgcccagcgt tgcccagcgc tggtgcacgt cgggttgact caggatgcgg gtgatttctc
      181 cgttaagcct gtcaatgatc gcacgcggcg tcttcgcggg aaccagcaac ccgagccagg
      241 ctacccagcg atacgcaggc aacccggact cggcaaccgt cggaacgtca gggagcagag
      301 agctgcggcg cgtggtggtg accccgagcg cctgcacttt gccgtccttc acctgcggta
      361 gcgcgagtcc caggggcgcc atgaacaact gcacgcgccc ggccatggtt tcggtgagcg
      421 cctcgggtat gcctttgtag ggcacgtgca cgatatcgat gcctgccatg ctcttgaaaa
      481 tctccatcgc gaagtgcgta ttgctgccga ccccggcgga agagaaattg agctgcccgg
      541 gcctggccct ggcgagcgcg atgaggtcct tgaccgattt gacgcccagc gacgacgagg
      601 cgaccagcaa catggcattg gtgcccattg tggtgacgcc ggcgaaatcc cgcacggcgt
      661 caaacggcag cttggcgtag agggcgggcg tagcggcgtg cgccgaagtc accgcgagca
      721 tcgtgtaacc gtcgggagcg gaaccggcaa cgatctggga cgcaacgatg ccgccggcgc
      781 ccggccggtt ttccaccacc accggttgcg tccagctttc ggcaagtttc tgggcaatga
      841 cgcgtgccat gatatccggc aagccgcccg gggaaaagcc gatcacgatg cggaccggct
      901 tgctcgggaa atccggtgct gaacccgtgg aagagccccg cgccggcgca gcgtgaatgc
      961 cgccagtcat cgcagcgcac agagaaacgc cgaggaaagt cttccagcat gctttcattg
     1021 cgcttctcct tggggtgctg acgataacga atcgcgcgta acgccgtgac gattgtagca
     1081 acccgcccgt atcgattggt gcggatgcgt tgccgcgtgg tcgaatcccg gttattttac
     1141 ggcgcagccg acatgtaacg ggagaatacc gatggcgcgc gatccccagg ttgacctcat
     1201 cttttcacat ttcacgccgc gatacatcgg cacgggagtc gatccgaacg atttgaaccg
     1261 cttgatggca gggatcgatg attgggcgga gtggtgccgt atctggtcgg atgaagccgg
     1321 ccggcacgaa aggctggcga ccgaagctgc cgaaaggaac cggcgcgtga ccgctgcaga
     1381 aggctttctc cgcgcagcga tctattacca ttatgcgaag cacctgttcg ccaattcgcc
     1441 ggaggagtac cggacggcgc acgataacat gttgcgttgc tacatggccg ccgcccccgg
     1501 taccgatccg ccgatggagc gcatcgaatt tccgttcgaa ggcgggctca tgccgggcta
     1561 tctccgccgc ccgcgcgggg tggagcggcc accggttgcg atcatccttc ccggcctcga
     1621 tgcgtgcaag gaggaactcc atgcgtggag caatgccttc ctcaagcgcg aacttgccac
     1681 gctgaccctc gatgggccgg gccaggggga gacggcttac tggctcccga tccgttccga
     1741 ttgggggcgt gtcatcggtg ccgtgatcga cgttctggag caacgacccg acgtcaatgg
     1801 gtacgcagtc ggcatcgtcg ggcaaagcct cggtgccctt tacgcgccgc tcgcggcggc
     1861 gggtgaatcg aggatcaagg cctgcattgc caactgcggt ccctttgatt gggcggctct
     1921 gctgccgaag atgccgaaac tgtcccagga ggtattccgg gcccgcagcc atgcctctac
     1981 gccggcccgg gcgcgggaga tcgcatcgca gctcacgctc gagggtgcag ccgaacggat
     2041 cagatgcccg ctgctggtcg tattcggcgg aggcgaccgg ttggtatcgc cctccgaagg
     2101 ggagcgtctc gcgcgtgccg cgtcgggtcc gaccgaattc gtggtctacg aggaggggaa
     2161 tcacgtctgc ttcaacatct gttacaaatt tcgcccgctg acgggcgatt ggatggcaga
     2221 gcgcttggcg gccaaaggct gacggagggc cgatactcag tcgcgtcact tcatcaccgc
     2281 cgttggatcg gagttcaccg gcggttcaga gcggccgcgt atcgtgattc gagaatcgtg
     2341 attcgactaa acccgcttta tcggtcaccg cgcgcgtcaa tcaacgcgtg ctcccgaggc
     2401 cttgacgatc tttccccaca ccccgacttg ttcccgcagg aacgcgtcgc actgctccgg
     2461 cctcgatccc acgagttcga tatccgcttg ggcgagtcgc gcacgcacgt ccggcgcgtg
     2521 cagggccttg acgatgtcgg cgttgagctt gttgagcacc gcagtcggcg tgccggtgcg
     2581 cgtgaccagc gcccagaagc ccgtgccggc ctggtagttc gggagcgttt ccccgacggt
     2641 cgggacgccc ggaagtgaag gcgcgcgctt ggggctcgtg gtggcgatta ccctgagctt
     2701 gcgcgactgg atgtagggca ccgcctccac cagcacagtg gcgagcagcg ggatacggcc
     2761 gggcagcacg tcgaggaccg cctggctgct gcccttgtag ggcacgtgca cgatattgat
     2821 gccggcggca aggcggattg cctcggtcgc gacgtggttt gcagtgcccg atcccccggt
     2881 ggcgtagttg atttcacccg ggcgcgcctt ggcgagcctg accagctcgg ccatgttctt
     2941 cgccggaaac gaaggatgtg cgagcagcac cccgtagagg ttgccaatgt gcgtgatgca
     3001 gccgaagtcc ttgagcgtgt cgtacgggag cctggcgtag agcgtggcat tgatcccgaa
     3061 cggcgtgatg gcgtgcagga tcgtatggcc gtccgccggg gccttggcga cgaactcggt
     3121 cgcgatcgtg gtgccgccgc cggggcgatt ttccacgacg acttgctggc cccaggcatc
     3181 cgccagctgc ttcgccagga cccgcccgaa gaggtcggtc tcgccgcctg cgggcagcgg
     3241 catgacgtag cgcagcgggc gggtggggaa attctgcgag ttcgctccgg cagagacaaa
     3301 catccccagc aaaagacccg cgcaaaccgc gaggcgcaat ggtcgcatgg catccccctc
     3361 cccagcgttg ttgtgcctac tgacctggtc aatttagcag caaccggcgg ggtgtgcaaa
     3421 acgatgcagc gtgagtgata atgccccggg caccgacaaa gggagacgga cgatgccagt
     3481 gctggcgttg ggttacatcg gcgttgacgc gaccgatctc ggcgcgtggc gcgcgttcgg
     3541 gaccgacgtg ctggcgatgc agatcgtcga gcgcggcgcg gacgcgctcg cgctgcgcat
     3601 ggatgagcgc tgccagcgcg tgctggtgca gcgcgccgag cgcgacgggc cggcgttttt
     3661 cggattcgaa acgaccggcg agcaggcgtt gaaggagacc gtcggcaagc tcgaggcggc
     3721 aggcgtctcg atgacgcgtg ccagcgagaa ggagctgatg ctgcgccggg tggacgacat
     3781 ggcctggtgc cgcgacccgg cgggcaaccg cgtcgagttc tatcacggcc aggcggcggg
     3841 caacggaccg ttcatgccac cgcgcccgca cgggggcttt cgcaccggac ggctgggcat
     3901 ggggcacatg gtgctgtatg ccgaggacat cgacgccatg agcgcgttct accgcgagct
     3961 gctcggcttg cggctctccg actacatgat caagccgtac cgcctgatgt tcctgcgcgc
     4021 caacccgcgc caccacagcg tcgggctgct gcaatcgaag cacaacggcg tgcaccacgt
     4081 gatgttcgag gtgctcgcgc tcgacgacgt cggccgtgcc tacgatttcg cgctggaggg
     4141 ctggcgcatc ggccagacgc tcggccggca cgccaacgac tggatgcttt cgttctacgc
     4201 gttctgcccg gccggattca tgatggaata cggctggggt gggcgcaccg tggacgaagc
     4261 gtcctggacg ccgcgcgaag tcacgcacgg cggcagcatg tggggccacg accggctatg
     4321 gttggacgag
//
LOCUS       DBNGWZ010000315         2882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_412889, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000315 DBNGWZ010000000
VERSION     DBNGWZ010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_412889"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16CX_09160"
     CDS             complement(<1..746)
                     /locus_tag="AC16CX_09160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012717.1"
                     /translation="MARELDGKVALVTGAVRRNGRAIAHALARDGAAIVINTRRSRDE
                     AEKVRAEIEALGRPALVCLADVTDEAAVARMFGDVEKRFGRLDILVNNAADRSVVPFE
                     KMTTEQWRHIVGICLDGAFFCTRAAIPHMLKNGWGRIVNISGVSHLAPTYTGRAHVAA
                     AKAGVEGFTRALAAEFAKKNITVNCVCPGRIGGERSASAAEMPDPALVPPVGHLGVPE
                     DIAGAVHYLCLPGSGFVTGHTIHVNGGQVL"
     gene            complement(754..1755)
                     /locus_tag="AC16CX_09165"
     CDS             complement(754..1755)
                     /locus_tag="AC16CX_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012718.1"
                     /translation="MKKPMAAAITIALAMALPALAQQAKPAAGVGAGSYPSKSIRLVV
                     PSAPGGGTDIIARLIAQGLTDSWGHTVVVDNRGGAGGIAGVTIVAKQSAADGHTMLLG
                     SVGHLTFAPAIERNLEYDPQKDLTPISLAAIQPFVVGASRSLPANTIQELIALAKAKP
                     GTISYGSGGSGTASHLGVELMMLNAGFSMLHVPYKGSNPAVIAMMSGELQIALAGLAT
                     LLPHVKSGKVKALAVTGAKRAQFAPEIPTVAESGIPGYVFDVWYGLVFPGGTPRAIVT
                     KTNAEVVRLLKSPEVNSRFAGAGVEPITNTPEAFAEMIRQEIPKWHKVVKAARIRVD"
     gene            1900..2337
                     /locus_tag="AC16CX_09170"
     CDS             1900..2337
                     /locus_tag="AC16CX_09170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3012719.1"
                     /translation="MKRRTTKRDYGDRFERGMRVLKKMGREKLMLNQKALYPEMYEMS
                     VGHLFGDVWGRPGLSLRDRQLVTLAANIALARPTGNHSHYLSALHLGITKREICEVMI
                     QVGHYAGWPALSNAVRQFTDILEEQRKMEQAKKLGRPFKSRLL"
     gene            complement(2421..2723)
                     /locus_tag="AC16CX_09175"
     CDS             complement(2421..2723)
                     /locus_tag="AC16CX_09175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3012720.1"
                     /translation="MVLEVAILHIKPEEIKNFEAVFPKVEPIFRSVPGYISHELQRCV
                     ETKGRYHFLIRWETIESHTVNFRQSPKFQEFRGLVGGFFDKPPVAEHFERVTESHI"
BASE COUNT          518 a          937 c          902 g          525 t
ORIGIN      
        1 atgaggacct gcccaccgtt gacgtggatc gtgtgcccag tgacgaaccc cgagccgggc
       61 aggcaaagat aatgtactgc gcccgcgata tcctcgggca cgcccaggtg cccgaccggc
      121 gggaccagag cggggtcggg catctcggcg gcgctcgcgg agcgctcacc gccgattcgt
      181 ccagggcaaa cgcagttcac cgtgatgttc ttcttcgcga actccgcggc gagggcgcgc
      241 gtgaatcctt ccactcccgc tttcgccgcc gcgacatgcg cccgcccggt ataggtcggc
      301 gccaagtggc tgacaccgct gatgttgacg atgcggcccc agccgttctt cagcatgtgc
      361 ggaatcgccg cgcgcgtgca gaagaaagct ccgtcgaggc aaataccgac gatgtgccgc
      421 cattgctccg tcgtcatttt ctcgaacggc acgacgctgc ggtcggcggc gttgttgacg
      481 aggatgtcga ggcggccgaa gcgtttttcg acgtcgccga acatgcgcgc gaccgcggcc
      541 tcgtcggtga cgtcggcgag gcacaccagg gccgggcggc ccaacgcctc gatctccgcc
      601 cgcactttct ccgcctcgtc gcgcgagcgg cgcgtgttga tgacgatcgc cgcgccatcg
      661 cgcgcgagcg cgtgcgcgat ggcgcgcccg ttgcgccgca cggcgccggt caccagcgcg
      721 accttgccgt cgagttcccg ggccatcgtt cacctaatcc acacggatcc tggcggcctt
      781 caccaccttg tgccatttcg gtatttcctg cctgatcatc tcggcgaagg cttcaggcgt
      841 gttggtgatc ggctcgacgc cggcgcccgc gaaccggctg ttcacctccg gcgatttcag
      901 cagcctcacg acttccgcgt tggttttcgt gacgatggcg cgcggcgtgc cgcccggaaa
      961 taccaatccg taccacacgt cgaatacata tccgggtatc cccgactccg caaccgtcgg
     1021 tatttccggc gcgaactggg cgcgcttcgc cccggtcacc gcgagcgcct tgaccttccc
     1081 gcttttcacg tgcggcagca gggtcgccag gcccgccagc gcgatctgga gctcgccgct
     1141 catcattgcg attacagccg ggttgctgcc cttgtacggc acgtgcagca tcgagaagcc
     1201 ggcgttgagc atcatcagct cgacacccag gtgtgacgca gtgccgctgc cgccggagcc
     1261 gtagctgatc gtgccgggtt tcgccttcgc cagcgcgatc aactcctgga tcgtattggc
     1321 cgggagggag cgcgacgcgc ccaccacgaa cggctgaatc gcggcgagcg agatgggggt
     1381 gagatccttt tgcggatcgt actccaggtt gcgttcgatc gcgggcgcga aagtcaggtg
     1441 gcccacgctg ccgaggagca tggtgtggcc gtcggcggcg gattgcttgg ccacgatcgt
     1501 gacgccggca atgccgcccg ctccgccgcg attgtccact acaacggtat ggccccagct
     1561 gtcggtcagc ccctgcgcaa tcaggcgcgc aatgatgtcg gtgccgccgc cgggcgcgct
     1621 cgggacgacc aggcggatcg atttgctcgg gtagctgcct gcgcccacgc ccgcagcggg
     1681 cttcgcttgt tgcgcgagcg cgggcagcgc catcgccagc gcgattgtga ttgccgcagc
     1741 catcggtttt ttcactggca cctcctcatg gaccggcact gccgcgcgcc ggtggcagac
     1801 gcttgctccg cgcgtgccgc aatgacatga tcacacaaaa gctgcgacaa aaaaacaacg
     1861 ctcctctcaa ccgggttcca tactgtgaga ccaaccatga tgaaaagaag gacgacgaaa
     1921 cgcgattacg gcgaccgctt cgagcgcggc atgcgggtgc tgaagaagat gggccgtgaa
     1981 aaactgatgc tgaaccagaa agcgctctat ccggagatgt acgagatgtc ggtcgggcac
     2041 ctgttcggcg acgtctgggg acgccccggg ttgagcctgc gcgaccggca gctcgtgacg
     2101 ctcgctgcga acatcgccct ggcgcgcccg accggcaacc attcgcatta cctgagcgcg
     2161 ctgcacctcg gcatcaccaa gcgggaaatc tgcgaggtaa tgatccaagt cgggcactat
     2221 gccggctggc cggcgctctc caacgccgtg cgccagttca ccgacatact cgaagagcag
     2281 aggaagatgg aacaggcgaa gaagctgggc aggccgttca agtcgcggct gctctagccg
     2341 cagcggtcag ccctcggcaa tcagccgtca gccagattgt cgtttggtcc ggctattagc
     2401 tgatagctga tagccgcctc tcagatgtga ctctcggtca cccgctcgaa atgctcggcg
     2461 accggcggct tgtcgaagaa cccgccgacc aggccgcgga attcctgaaa cttcggcgac
     2521 tgccggaagt tgaccgtatg ggattcgatg gtctcccagc ggatcaggaa gtggtagcgg
     2581 cccttggtct cgacgcagcg ctgcagctcg tgcgagatat agcccggcac gctgcggaag
     2641 atcggctcga ccttgggaaa aacggcctcg aaattcttta tttcctcagg cttgatgtgc
     2701 aggatggcga cttccagaac catgattgtt ctcctatcag ttaatgacgt ctagaagctg
     2761 tcgagaagat ccgaatgaaa ccgcagatga acgcagatga acacggatat tcgaatcccg
     2821 aagatggcat cagcaggggc ttgcacccgc gttcatccga acccgctaga tttcgtccac
     2881 ct
//
LOCUS       DBNGWZ010000316         4632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_288777, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000316 DBNGWZ010000000
VERSION     DBNGWZ010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_288777"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16CX_09180"
     CDS             complement(<1..1008)
                     /locus_tag="AC16CX_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR3012721.1"
                     /translation="MSSNRIGICLGCLWLLLALTALPVAAETVRIYATNSAGDNIHVI
                     DASTNKVVQVIKGIETPHGVDFSPDGTRVYVSNEADSTLDVVDRKTGKVIKKVPLTGH
                     PNNIAVTRDGGRVIICIARNDGGLDIVNTTSLTRTKFIPLNGRMHNVYVTNDNKYAYA
                     GSVRQKFLAVLDLQTEAVAWDLKFDKGVRPMTAEHNPDGSASRIFIQLSDTSGFAVVD
                     FAQRKEVARILFPDQPGGYGKAEGRIGTPSHGMSVAPDNKSLWVTSVFANAVFVYSLP
                     DLKLQGHVTLPDLRLPGREPIGALPNWTTFTPDGRYLYVTNSAINSVSVVDTKAMKVV
                     AN"
     gene            complement(1317..2294)
                     /locus_tag="AC16CX_09185"
     CDS             complement(1317..2294)
                     /locus_tag="AC16CX_09185"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012722.1"
                     /translation="MKTALRRFILFLLCVSSAVYGISAGAQAYPTRPIRAIVPFAPGG
                     ATDAVIRMIAPRLSEYLGQQVVVENRPGGAATIGMDVVAKSPPDGHTMGMANLTFSIN
                     PILFKKMPFDSERDLALVSHISILPFLVGVHPSVPAKSIKDLIALARARPGALNYSSS
                     GQASATQMATELFKYLTKTDMVHIPFQGGGPALVAVLSGEVSVYWGTIPPLLAHMKSG
                     RLRVLGVTTLQRDPAIPDVPTVAESGVPGYQAAEWGGLVVRAGTPAAIINRLNQEIVK
                     SLKSPEIAQRFAAVGAQAVGSTPEGFAEHIRRERETWGKVIQVAGIRIE"
     gene            complement(2501..>4632)
                     /locus_tag="AC16CX_09190"
     CDS             complement(2501..>4632)
                     /locus_tag="AC16CX_09190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012723.1"
                     /translation="TMSTAAKLPSTLAANPRLDRWVTLNADGTVTVRTGKVEIGQGIV
                     SAMAQIAAEELDVAYSRIRMVAVDSSMSPNEGSTSGSRSIEEGGGALRQACAEVRDAL
                     VQAGARKLGASLESLAVEDGTIRARGGGSITYWQLAPEVDLARDATGQVRPKETEELR
                     LIGTALPRLDIPAKVSGAAFLHDLDLPGMLHGRVVRPPSYRARLVSLDDAHVRAMPGV
                     RAVVREGRFLGVVAGREEQAVKAREALARAVQWKEPEALPDESAIHAYLQAQPAEEEV
                     LCEKSSARTVAGATLFATYTRPYLAHASIGPSCAIAWWRSGRLEVWSHSQSIHALRDE
                     LAKILGIPATDVIARHAEGAGCYGHNGADDVALDAALLARAVDGSPVRVQWMREDEFA
                     WEPFGPAMAVKLRGALDAGGNVAEWREDIWGNRHITRPGRHPRPGLLAAWHRDEGVEP
                     PPAVDMPLAMGGGSQRNAVPYYDFPNERVVNHAVKAMPLRVSTLRSLGAYLNVFAIES
                     FMDELAHAAGSDPVAFRLRHLKDERARAVIRAAAEKAGWKPGAKGDGMRGMGIGFARY
                     KNIGDWVAVVAEVVVEEVVRVPRVVAAIDCGRVVNPDGVVNQAEGGVIQAVSWTLKEQ
                     VRFDRTRVTTRNWEDYPILTFEEAPTVEVVLLDRADEPSLGVGEGMMGPTAAAIGNAV
                     FNAMGVRVRDLPLTPERVAAAIHAGHE"
BASE COUNT          742 a         1543 c         1524 g          823 t
ORIGIN      
        1 gttcgccacc accttcatcg ccttggtgtc gacgacggac accgaattga tcgcggagtt
       61 ggtgacatag agataccggc cgtcgggcgt gaaagtggtc cagttcggca acgccccgat
      121 cggctcgcgt cccggcagcc tgagatcggg aagcgtgacg tgtccctgga gcttgaggtc
      181 gggaagcgaa tagacaaaga cggcgttggc gaagacgctg gtgacccaca gcgacttgtt
      241 gtccggcgcg acgctcatgc catgggaggg cgtgccgatc cttccctcgg ctttgccgta
      301 gccgccgggc tggtcgggaa acaggatgcg cgccacttcc ttgcgttgcg caaaatccac
      361 gaccgcgaac ccgctggtgt ccgagagctg gatgaaaatg cggctggccg agccgtcggg
      421 attgtgctcc gccgtcatgg gacgcacgcc cttgtcgaac ttgagatccc acgccaccgc
      481 ctcggtctgc aggtcgagga cggccaggaa cttctgccgg accgagcccg cgtacgcgta
      541 cttgttgtcg ttcgtcacgt aaacgttgtg catgcgtcca ttgagcggga tgaacttggt
      601 gcgcgtgagg gaggtggtgt tgacgatgtc gagcccgcca tcgttcctgg cgatgcagat
      661 gatgacccgg ccgccgtccc tggtgactgc gatgttgttg ggatggccgg tcagcggcac
      721 tttcttgatg actttgccgg tcttgcggtc cacgacgtcg agcgtgctgt cggcctcgtt
      781 gctgacatag acgcgcgttc cgtccggcga gaagtcaacg ccatggggcg tctcgatgcc
      841 cttgatcacc tgcacgacct tgttcgtgga agcgtctatg acgtgaatgt tgtcgccggc
      901 gctgttggtc gcgtagatgc ggaccgtttc cgccgccacg ggcagcgccg tcagcgcgag
      961 cagcagccac aggcagccaa ggcatatgcc gatgcgattt gagctcatgg gcgaactcct
     1021 ctcggtggac cggaaaagtc taggcccgcg cacgctcatt agcaagggtc cggaccccgt
     1081 gaggagtgag ggagagtagc ttaggctcaa gttgaaagtt tcaggttgca aggttcaaga
     1141 tgcaagggtt cacgtttacc ttgcatcttg tgacttgtac cttgtaactc gcaccttttg
     1201 acttgtgcct tgcccctgtt cctgaaaggt ccctggtgac gcagtgacgt ggtaatgggg
     1261 cctgcccgag gggtgagtgc ggaggactga ggaggttact cgtcactcgt cactcgtcac
     1321 tcgatgcgga ttcccgccac ctggatcact ttcccccacg tttcgcgctc gcgcctgatg
     1381 tgctcggcga atccctcggg cgtgctgccg accgcttgtg cgccgaccgc cgcgaagcgc
     1441 tgggcgattt cgggcgactt caggctcttc acgatttcct ggttgaggcg gttgatgatg
     1501 gcggccggcg tgccggcgcg cacgacgagg ccgccccatt cggcggcttg atatcccggc
     1561 acgccggact ccgcgaccgt cggcacgtcg gggatcgccg ggtcgcgctg caaggtggtc
     1621 acgccgagca cgcgcagccg gccggatttc atgtgcgcga gcagcggcgg gatggtgccc
     1681 caatagaccg agacctcgcc gctcagaacg gcgaccagcg cagggccgcc gccctggaac
     1741 gggatgtgga ccatgtcggt cttcgtcagg tacttgaaga gctcggtggc catctgcgtt
     1801 gcgctcgcct gtcccgatga ggaatagttg agcgcgcccg gccgcgcccg agccagcgcg
     1861 atcagatcct tgatcgattt cgccgggacc gaaggatgca cgccgaccag aaacggcagg
     1921 atgctgatgt ggctgacgag agcgaggtcc ctttcgctgt cgaacggcat cttcttgaac
     1981 aggatcgggt tgatgctgaa cgtcaggttg gccatcccca tggtgtggcc gtcgggcgga
     2041 gacttggcga cgacgtccat gccgatggtg gcggcgccgc ccggccggtt ctcgaccacg
     2101 acctgctggc cgagatactc tgaaagccgc ggcgcgatca tgcggatcac cgcgtcggtc
     2161 gcgccgcccg gcgcgaacgg cacgatcgcg cggatcggtc gcgtcgggta ggcctgcgcc
     2221 cctgcggaaa tgccgtaaac ggcggacgac acgcacaaca gaaacaggat gaagcggcgt
     2281 aacgcggtct tcatgatctt tcctccctgg aagcggtaaa cgatatctta agcccgtggg
     2341 tcgggattcg tgaatcgcaa ctcgaactgc gtagaaaggc gtgataagtg gcgagtgata
     2401 agtgataagt ctcccgcgca gcgggggctc ttaactccta tcacctatca cttatcacct
     2461 atcatgcttt cgggtttgcg ttctcgtcac tcgaccctcg tcactcgtga ccagcgtgaa
     2521 tggctgctgc cacgcgttcg ggcgtgagcg gcagatcccg cacgcgcacc cccatcgcgt
     2581 tgaacacggc gttgccgatg gcggccgccg tcggccccat catgccctcg cccacgccga
     2641 gcgacggctc gtcggcgcga tcgagcagga ccacctcgac tgtaggcgcc tcctcgaacg
     2701 tcaggatcgg ataatcctcc cagttgcgcg tcgtgacacg cgtgcggtcg aagcgcacct
     2761 gctccttgag tgtccagctc accgcctgga tgacgccgcc ctcggcctgg ttgaccacgc
     2821 cgtccgggtt gacgacgcgc ccgcaatcga tcgccgcgac gacgcgcggc acgcgcacga
     2881 cttcctcgac cacgacctcg gcgacgactg cgacccagtc gccgatgttc ttgtagcgcg
     2941 cgaagccgat gcccatgccg cgcatgccgt cgcccttggc gccgggcttc cagcccgcct
     3001 tctccgccgc cgcccgaatc actgcgcgcg cccgctcgtc cttcaggtgc cgcagccgga
     3061 acgctaccgg gtcggagccc gcggcgtgcg caagctcgtc catgaaggac tcgatcgcga
     3121 agacgttgag gtacgcgccg agtgaccgca acgtagacac ccgcagcggc atcgccttca
     3181 ccgcatggtt caccacgcgc tcgtttggaa agtcgtagta cggcacggca ttgcgctggc
     3241 tgcccccacc catcgcgagc ggcatgtcca ccgcgggagg cggttcgacg ccttcgtccc
     3301 ggtgccacgc cgcgagcagg ccgggtcggg gatggcgccc gggacgggtg atgtgccggt
     3361 tgccccagat atcctcgcgc cattcggcga cgttgccgcc cgcatcgagc gcgccgcgca
     3421 gcttcacggc catcgcgggg ccgaacggct cccaggcgaa ctcgtcctcg cgcatccact
     3481 gcacgcgcac cggactgccg tccaccgcgc gcgcgaggag cgcggcatcg agcgccacgt
     3541 cgtccgcccc gttgtgaccg tagcagcccg cgccttccgc gtggcgcgcg atgacgtcgg
     3601 tggcggggat gccgagtatc ttcgccagct cgtcacgcag cgcgtggatc gactggctgt
     3661 gcgaccagac ctcgagcctg cccgagcgcc accaggcgat cgcgcaggag ggcccgatcg
     3721 aggcgtgggc gagatagggc cgcgtgtaag tagcgaagag cgtcgcgccc gccacggtgc
     3781 gcgccgagga cttttcgcaa agaacctctt cctcggccgg ttgcgcctgc aggtaagcgt
     3841 ggatagcgct ctcgtccggc agcgcttccg gctccttcca ctgcaccgcg cgcgcgagcg
     3901 cttcccgcgc cttcaccgcc tgctcctcgc gcccggcgac gacgccgagg aaccggccct
     3961 cgcgcaccac tgcgcgcacg ccgggcatcg cccgcacatg cgcgtcgtcc agcgagacga
     4021 gacgcgcgcg atacgagggc gggcgcacga cgcgcccgtg cagcatgccg ggcagatcga
     4081 ggtcatgcag gaaagccgcg ccgctcacct tggcgggaat gtcgaggcgc ggcagcgctg
     4141 tcccgatgag ccgcaactct tcggtctcct tgggccggac ctgccccgtc gcatcgcgcg
     4201 cgaggtctac ttcgggcgcg agctgccaat aggttatgga gccgccgccg cgcgcgcgta
     4261 tcgtgccgtc ctcgaccgcg aggctctcga ggctcgcgcc gagcttgcgg gcgcccgcct
     4321 gcaccagcgc atcgcgcacc tccgcgcacg cctgccggag cgctccgcca ccctcctcga
     4381 tcgagcggct gcccgaagtc gagccttcgt tcgggctcat gctggaatca accgcgacca
     4441 tacggatgcg cgagtaggcg acatcgagtt cctcggctgc gatctgcgcc atcgcggaga
     4501 cgatgccctg cccgatctcg accttgccgg tgcggaccgt gaccgtgccg tcggcgttga
     4561 gcgtcaccca gcggtcgagc ctgggattgg cagccaaagt cgaaggaagc ttagccgccg
     4621 tgctcatggt ga
//
LOCUS       DBNGWZ010000317         5190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2429518, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000317 DBNGWZ010000000
VERSION     DBNGWZ010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2429518"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="AC16CX_09195"
     CDS             <1..440
                     /locus_tag="AC16CX_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012724.1"
                     /translation="HQKVTGAVARFDALLEAFEFVSIGRPGEQEAYLCTETGEIHWHS
                     EYGDNEEPLPEDIEDAQKYIAIPHKNDLGLGKPLVLKFAREFLTDVLDDVHEIFRRRG
                     AYARFKDLLENRGLLQRWYEYEANAQQKALREWCADRGIELEG"
     gene            complement(516..1103)
                     /locus_tag="AC16CX_09200"
     CDS             complement(516..1103)
                     /locus_tag="AC16CX_09200"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3012725.1"
                     /translation="MRTLERAPAGNTPEEKRVLAVLDGVYRNQRYLSVPEEDGRLLRI
                     LAESVGAGSVVEIGTSTGYSGLWLLLALMKTGGRLTTYEIDRGRHELARHNFERAGLL
                     GHATLVLGDAHAEVAKLKGPVDLVFLDADKEGYPDYLQKLAPLTRPGGLIVAHNMASP
                     PPDSRYVEAVTTNPAFDTVFLNMHAAGVGVTLKKR"
     gene            complement(1318..1911)
                     /locus_tag="AC16CX_09205"
     CDS             complement(1318..1911)
                     /locus_tag="AC16CX_09205"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3012726.1"
                     /translation="MLTTTRFGRTFAAVALTSGALAAVVPFNLYGDPPPWAPAHGWRK
                     KNDPYYTGYTGKRWGKDYGVLDGRCNRAAVGTVIGGVAGGVIGSEVGRGGDRQIAIVV
                     GTIAGAIIGAQIGRDMDQADRACMGHALELVGDRRRVSWQSADNRTSYLLTPVHGFEH
                     NGAHCREFDFTVTSRGRKETNRGMACPTGDGGWRILD"
     gene            complement(2045..>5190)
                     /locus_tag="AC16CX_09210"
     CDS             complement(2045..>5190)
                     /locus_tag="AC16CX_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HIR3012727.1"
                     /translation="DNNLSYCEMIGSTNPCVTGDTRLHTQHGLVSIGELHSTGVPLQA
                     TVDVRALGQVDRGVMLRPAAPAFMTAGAADVYRVETVDGYEIKATGWHDFYTTRGKIK
                     LKDLKPGDELLVQSGKGQFGSEGNGEIGLLIGLITGDGHFTNRGNGNEAAIINFWGED
                     REFASAVANQVNSAIAGLAVTPRTYRVAPTAIPARNQVFVRSVLLARFLEHYGFTAAT
                     KLRVPEVVWRGTEDCVKGYLRGLFQTDGTVNVSGLSQSCSVRLASSEPCLLKDVQVLL
                     ANFGIFCRIRKRRDPSSRWMPDGKGGQRLYACRADYELIIDGESRERFMQEIGFHSEA
                     KKVKYRQWVKDKVLRKSQSFVTKVAAITYIGREAVYDTTQADHNTVIFNGLVTGQCGE
                     QPLPAYGCCCLGSVNLAPFVREPFGAKARFDFDAFGKVVHPSVRMLDNVLDATVWPIE
                     QQQAEARAKRRVGLGFTGLGDALIMLGLRYDTDEARRMAARITERMRDEAYAASVELA
                     KEKGAFPLLDAEQHLAAPRFASRLPEKLKSEIRKHGIRNSHLLSIAPTGTISLAFADN
                     ASNGIEPAYAWTYQRKKRMPDGSMKTYDVEDHAWRLYRHLGGDVEKLPPHFVTAMDIS
                     ALDHMRMVAVAAPYIDSAISKTVNVPEDYPYEQFKDLYLEAWKAGLKGITTYRPNKVL
                     GSVLSVTPQDEQPNDLDISDTDRRLRLEAAPQPALSSLRWPGRPKLAAGNPSWTYMVE
                     SPFGQFAIFVGHVENAGPHPFEVWVNGTEQPRGLGAVAKTLSMDMRAQDKAWLRMKLD
                     ILARTAGDDAFDCPMPPDGEQQRVPSVVAAFARIVRYRVDELGALSKSDGKSPVLDAL
                     YARKEPKTGPSGTMSWTADVHNPRAEDDFVLILKELQLPNGQRRPYSVWMAGEYPRAL
                     DGLCKLLSLDMRVIDPAWIGMKLRKLLNYSEPLGDFMARVPGEQRQQNFPSTIAYVAR
                     LIIHRYAMLGVLDERGYPLNEMGVLEIPEDDRMEARRVQYRVLYGKKCKECGNTAVIY
                     KDGCEFCTACGAIGACG"
BASE COUNT          841 a         1696 c         1709 g          944 t
ORIGIN      
        1 gccatcaaaa ggtgaccggt gcggtggcaa ggtttgatgc gttactcgaa gccttcgagt
       61 tcgtcagcat cggccgaccg ggggagcagg aagcgtatct ctgcacggaa accggcgaga
      121 tccattggca cagtgagtat ggggacaacg aagagccgct gccggaggac atcgaggacg
      181 ctcaaaagta catcgcgatt ccgcacaaaa acgatctcgg cctcggcaag cctcttgtgt
      241 tgaagtttgc aagagagttt ctgaccgacg tgctggacga cgtgcacgaa atctttaggc
      301 gcaggggcgc ctacgcgcgc ttcaaggatc tgctggaaaa ccgcggcctg ctgcagcggt
      361 ggtacgaata cgaggcaaac gctcagcaga aggcgctgcg agagtggtgc gcggaccgcg
      421 gcattgaatt ggaaggctag cgtttcccgc gcgttgagcg gcgcgaccgc gtccgttcca
      481 atgcgcggag ttaaaccggc gcgcagcgtc cggctttaac gttttttgag ggtgaccccg
      541 acccccgccg cgtgcatgtt gaggaacacg gtgtcgaagg cggggttggt cgtgaccgcc
      601 tcgacatagc gcgaatcggg gggaggcgag gccatgttat gggcaacgat gaggccccca
      661 gggcgcgtga gcggcgcgag cttttgcagg tagtcggggt agccctcctt gtcggcgtca
      721 aggaagacga ggtcgacggg gcccttcagc ttcgcgacct cggcgtgcgc gtcgccgagc
      781 accagcgtcg catgaccgag caagcccgcg cgctcgaaat tgtggcgcgc gagctcgtgc
      841 cgcccgcggt cgatctcgta ggtggtgagc cgcccgccgg ttttcatcag ggcgagcagg
      901 agccacagcc ccgagtagcc ggtcgaggtg ccgatctcga cgacacttcc cgcgccgacg
      961 gactcggcga ggatgcgcag cagccgcccg tcctcctccg gcacgctcag gtagcgctgg
     1021 ttgcggtaga cgccgtccag caccgcgagg acgcgctttt cctccggcgt attgcccgcc
     1081 ggcgcgcgct cgagcgttct catgcgctgg gcgacggcag acgatacggg cagagctgcg
     1141 gcagcggtac ccagcgcaag cgaggccccc agcgccgccc gccgccccgg cctaaaggtt
     1201 tttttcacaa cgggttgcga ctatgcgcgt ttatccgcgt tcatctgcgg tttcgttctt
     1261 gttcctgcct tttctctgca tcccccgcgt cctcagcggt gagaggtcat accagcgcta
     1321 gtcgaggatg cgccatcctc cgtcgcccgt cgggcaggcc atcccccgat tggtctcttt
     1381 gcgcccgcgc gaggtcaccg taaagtcgaa ctcgcggcaa tgcgcgccgt tatgctcgaa
     1441 gccgtgcacc ggcgtcagca gataactcgt gcgattgtcg gcgctctgcc agctgacgcg
     1501 cctgcggtct ccgaccagct cgagggcgtg acccatgcac gcgcgatcgg cctggtccat
     1561 gtcgcggccg atctgcgcgc cgatgatcgc gccggcgatg gtgccgacga cgatcgcaat
     1621 ctgccggtcg ccgcccctgc ccacctcgga accgatgacc ccgcccgcga cgccgccgat
     1681 caccgtgccg accgccgcgc ggttgcagcg gccgtccaat acgccgtagt ccttgcccca
     1741 tctcttgccg gtatagccgg tgtaataggg gtcgttcttc ttgcgccagc cgtgcgccgg
     1801 tgcccacggc gggggatcgc cgtagaggtt gaacggaacg actgcggcca gcgccccgct
     1861 ggtcaacgcc accgcagcaa atgtcctgcc aaagcgtgtc gttgtgagca tttttcctcc
     1921 tgatcgcgca tggatgacgt gcgcatgacg cgtttgaccg cgcgcggatg gccctgttct
     1981 tgggacggct gtcgccgggc agcgacagcc cggcgcagcg gatggacgcc ggcggaacgc
     2041 cgcgtcagcc gcaggcgccg atcgccccgc acgccgtgca gaactcgcag ccgtccttgt
     2101 agatcacggc ggtgttgccg cattccttgc atttcttacc gtagagcacg cggtactgca
     2161 ccctgcgcgc ctccatccgg tcatcctcgg ggatctccag cacgcccatt tcgttcaggg
     2221 gatagccgcg ctcgtcgagc acgccgagca tcgcgtagcg gtggatgatg agccgcgcga
     2281 cgtaggcgat cgtgctcggg aagttctgct gccgctgctc tcccggcacg cgcgccatga
     2341 agtcgccgag cggctcgctg tagttgagga gcttcctcag cttcatgccg atccacgccg
     2401 ggtcgatcac gcgcatgtcg agcgagagca gcttgcacag tccgtcgagc gcgcgcgggt
     2461 actcgccggc catccatacc gagtacggcc ggcgctggcc gttgggcagc tgcagctcct
     2521 tgaggatcag cacgaaatcg tcctccgcgc gcgggttgtg cacgtccgcc gtccacgaca
     2581 tggtgccgga ggggccggtc ttgggctctt tcctcgcgta gagcgcgtcg agcaccggcg
     2641 attttccatc cgatttcgag agcgcgccga gctcgtccac gcggtagcgc acgatgcgcg
     2701 cgaacgccgc caccacgctc ggcacgcgct gctgctcgcc gtcgggcggc atcgggcagt
     2761 cgaaagcgtc gtcgcccgcg gtgcgcgcga ggatgtcgag cttcatcctg agccaggcct
     2821 tgtcctgcgc gcgcatgtcc atcgagagcg tcttcgccac cgcgcccagg ccgcgcggct
     2881 gctcggtgcc gttcacccac acctcgaacg ggtgcgggcc ggcgttctcg acatggccga
     2941 cgaagatcgc gaactggccg aacggtgact ccaccatgta ggtccacgat ggattgccgg
     3001 cggcgagctt cggacgcccc ggccagcgca ggctggagag cgcgggctgc ggcgcggcct
     3061 ccagccgcag gcggcggtcg gtatcgctga tgtcgagatc gttcggctgc tcgtcctggg
     3121 gcgtgaccga gagcacgctg cccaggacct tgttggggcg gtaggtggtg atgcccttca
     3181 gccccgcctt ccacgcctcg aggtaaaggt ccttgaactg ctcgtaggga tagtcctcgg
     3241 gcacgttgac cgtcttgctg atcgccgaat cgatgtaggg cgcggccacc gccaccatgc
     3301 gcatgtggtc gagcgcggag atgtccatcg cggtgacgaa gtgcggcggc agcttctcca
     3361 cgtcgccgcc caggtgccgg tagagccgcc acgcgtggtc ttccacgtcg taggtcttca
     3421 tcgaaccgtc gggcatgcgc ttcttgcgct ggtaggtcca ggcgtacgcg ggctcgatgc
     3481 cgttcgaagc gttgtcggcg aaggcgagcg agatcgtgcc ggtgggcgcg atcgagagca
     3541 ggtggctgtt gcggatgccg tgcttgcgga tttcgctctt cagcttttcg ggcaggcggg
     3601 aggcgaagcg cggcgcggcg aggtgctgct ccgcgtcgag cagcgggaac gcgccttttt
     3661 ccttcgcgag ctcgacggag gccgcgtacg cctcgtcgcg catgcgctcc gtgatccgcg
     3721 ccgccatgcg gcgcgcctcg tccgtgtcgt agcgcagccc gagcatgatg agcgcgtcgc
     3781 cgagcccggt gaacccgagc ccgacgcggc gcttggcacg agcctccgcc tgttgctgct
     3841 cgatcggcca taccgtcgcg tcgagcacgt tgtcgagcat gcgtaccgat ggatgcacga
     3901 ccttgccgaa cgcgtcgaag tcgaaacgcg ccttcgcgcc gaacggctcg cgcacgaacg
     3961 gcgcgaggtt cacgctgccg agacagcagc agccgtaggc cggaagaggc tgctcgccgc
     4021 attgcccggt aaccagcccg ttgaaaatca ccgtgttgtg atcggcttgc gtggtgtcgt
     4081 acaccgcctc acgcccgatg taagtgattg ccgccacttt ggtgacgaac gactgtgact
     4141 tgcgcaacac cttgtccttt acccactgcc ggtacttcac cttcttggcc tcggagtgga
     4201 agccgatttc ttgcatgaaa cgctcgcgcg actcgccgtc gatgatgagc tcgtaatcgg
     4261 cgcggcaagc gtacaaccgt tgtccaccct tgccatccgg catccagcgg ctgctcggat
     4321 cgcgtcgctt gcggatccgg cagaagatgc cgaaattggc aagcagcacc tgaacgtcct
     4381 tcagcaggca gggctcgctc gaggcgagac ggaccgagca gctctggctt aacccggaaa
     4441 cattgacggt gccatcagtc tggaacagac cgcgcaggta gccctttacg caatcctcgg
     4501 tcccgcgcca gacaacctcg ggcactcgca atttcgtggc agcggtgaac ccgtaatgct
     4561 cgaggaaacg ggcgagcagc acggagcgga cgaacacttg gttccgcgcg gggatggcgg
     4621 tcggggcgac ccggtaagta cgaggcgtca ccgcgaggcc cgcaatcgcg ctgttgactt
     4681 ggttggcaac cgcggaggcg aactcgcgat cctcgcccca gaaattgatg attgccgctt
     4741 cgttcccatt tccgcggttg gtgaagtggc catcacccgt gatcagaccg atcagcaacc
     4801 cgatctcgcc gttgccctcg ctgccgaact gccccttgcc ggactgaacc agcaattcat
     4861 cgcccggttt gaggtccttg agcttgatct tgccgcgggt ggtatagaaa tcatgccagc
     4921 cggtcgcttt gatttcgtaa ccgtccaccg tctcgacgcg atatacgtcc gccgcgcccg
     4981 cagtcatgaa ggctggcgca gctggccgca gcatcacgcc gcggtcgact tgtcccaagg
     5041 cgcgcacgtc aaccgttgct tgcagcggaa cgccggtcga atgcagttcg ccgatggaga
     5101 cgagcccgtg ttgggtatgc agccgcgtgt cgcccgtcac gcacgggttg gtgctcccga
     5161 tcatctcaca gtaggaaagg ttgttatccg
//
LOCUS       DBNGWZ010000318         2268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_541597, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000318 DBNGWZ010000000
VERSION     DBNGWZ010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_541597"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1405
                     /locus_tag="AC16CX_09215"
     CDS             197..1405
                     /locus_tag="AC16CX_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HIR3012728.1"
                     /translation="MQKSNVTAPLLAATLAGLVMLGPFSVDTILPSFPAIGREFALTP
                     LELQQTLSAYLVVFAMMTLFHGALSDSFGRRPVILGCLAIYVLGSIGCTFAQSFGQLL
                     LFRGAQGFSAGVGWVVGRAIVRESFAGHEAQRLLSLITMIFGLAPAVAPVIGGWLQGA
                     FGWRAVFVFLTLYGALQLAVCWLALPETHPREARQPFAAAPLLRTYLKLATSPLLVLL
                     CVAIAFNFAGFFLYVASAPAIIYGLLGLTELHFPVLFIPGIAGVMLGAFLSHRIAGRV
                     SRQRTVQLGYLVMFAAAAFNVGYHALYPAALPWTVLPYAVYAIGMALAAPSVQLLVLD
                     LFPRNSGTASSLQGFTHAIFTTFTAALISPLVSGSGATLAIGMFGLVTLGCVCWIGYR
                     RIESRVAARG"
     gene            complement(1718..>2268)
                     /locus_tag="AC16CX_09220"
     CDS             complement(1718..>2268)
                     /locus_tag="AC16CX_09220"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3012729.1"
                     /translation="MRLINAAVACGARLLVLPECGHAYGALRWQGANLYGKPLPFRVL
                     HISEFLAESVRSGRIRLKNLDASLTFHDPCQVSRRGGATQAPRDVIRALGAELREMPV
                     TGDANWCCGGGGGVVTIHRADDLRHRVFEIKMRQVEATGAEKLVSSCSNCRLTFDDGQ
                     VHYKWDKKMESLLELVAGQLAD"
BASE COUNT          329 a          757 c          723 g          459 t
ORIGIN      
        1 gcgatggtca tcgactttca gcatcatttc gcaccgcgcg agctggttga cgccaagccc
       61 ggcgccccgc gggtggtcaa gttcgacgcg accggcgcgc cggcgcacac atcgcattcg
      121 ctgctgatgc cgcgccttgc gcgcgcggcc gcctgagcca tcaccgggaa acgggcccga
      181 aaccggcaac acggccttgc agaaaagcaa cgttacggcg ccgttgctcg ccgccacgct
      241 cgcggggctg gtgatgctgg ggccgttctc ggtggacacc atactgccgt cgttcccggc
      301 gatcgggcgc gagttcgcgc tgacgccgct cgagctgcag cagacgctgt cagcgtatct
      361 cgtcgtcttc gccatgatga cgctgttcca cggcgcgctg tcggactcgt tcggacggcg
      421 cccggtgata ctcgggtgtc ttgcgatcta cgtgctcggc tcgatcggtt gcaccttcgc
      481 ccagagtttc ggccagttgc tgctctttcg cggcgcgcag ggcttctcgg caggcgtggg
      541 atgggtggtg ggccgcgcca tcgtccgcga aagctttgcc ggccacgagg cgcagcgcct
      601 gctctcgctc atcaccatga tcttcgggct ggcgccggcg gtggcgccgg tgatcggcgg
      661 ctggctgcag ggcgccttcg gctggcgcgc ggtattcgtg tttctcacgc tctacggggc
      721 gctgcagctg gcggtgtgct ggctggcgtt gccggaaacg catccacggg aggcgcgcca
      781 gccattcgct gctgcgccgc ttctcaggac ctatctcaag ctcgcaacca gcccgctgct
      841 ggtgctgctt tgcgtcgcca tcgcgttcaa tttcgcaggg ttcttcctct acgtcgcttc
      901 ggcgccggcc atcatctacg ggctgctcgg gctgaccgag ctccactttc cggtgctgtt
      961 catacccggc atcgccgggg tcatgctcgg cgccttcctg tcccaccgga tagcgggccg
     1021 cgtctcgcgc cagcggacgg tgcagctcgg ctacctcgtc atgttcgctg cggcggcctt
     1081 caacgtcggc taccacgcgc tgtatccggc ggcgctgccg tggaccgtgt tgccgtacgc
     1141 ggtctacgcg atcggcatgg cgctcgcggc gccgagcgtg cagctgctgg tgctcgatct
     1201 ttttccgcgg aacagcggca cggcctcctc gctgcagggc ttcacgcatg ccatcttcac
     1261 taccttcacc gcggcgctca tttcgccgct cgtctccggc agtggagcga cgctggcgat
     1321 cggcatgttc gggctggtga cgctgggctg cgtctgctgg atcggctatc gccgtatcga
     1381 gtcccgggtc gcggcgcggg gttgaaacca tagcttctct caaaaacacc ccgcaacctc
     1441 ggaaaccgcc ggtgaacgcc gatacacgca gacatattaa gagcaacccc aaggatttgt
     1501 tctgctttac atcagcgtta atcagcgatt atccgcggtt cacctgtttg ggttcttgag
     1561 tcaagtcatg gatttcaaag acaagaaaag caggctggcg gtagatggct gcggataaac
     1621 gccgaagagc atcgctgctg aggacttcgt cgggattcga actatccgtg ttcatctgcg
     1681 ttcatctgcg gtttcattcg gcttttcaag ataccttcta gtcggcgagt tggccggcga
     1741 cgagctcgag caagctttcc atcttcttgt cccacttgta gtgaacctgc ccgtcgtcga
     1801 aggtcagccg gcagttggaa cagctggaga cgagcttctc cgctccggta gcctccacct
     1861 gcctcatctt gatctcgaat acgcgatggc gcagatcatc ggcgcggtgg atcgtcacca
     1921 cgccgccgcc cccgccgcag caccagttgg cgtcgcccgt gaccggcatt tcgcgcagtt
     1981 ccgcgccgag cgcccggatc acgtcgcgcg gggcttgcgt cgcgccgccc cggcgcgaga
     2041 cctggcaggg gtcgtgaaag gtgagggagg cgtccaggtt cttcagccgt atacgcccgc
     2101 tgcgcacgct ctcggcgagg aactcggaga tgtgcagcac acggaacggc agcggcttgc
     2161 cgtacaggtt cgcgccctgc cagcgcagcg cgccgtatgc gtgcccgcac tccggcagca
     2221 cgagcagtcg cgccccgcac gccactgcgg cgttgatcag ccgcatgc
//
LOCUS       DBNGWZ010000319         4387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2934110, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000319 DBNGWZ010000000
VERSION     DBNGWZ010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2934110"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..2131)
                     /gene="tfpB"
                     /locus_tag="AC16CX_09225"
     CDS             complement(323..2131)
                     /gene="tfpB"
                     /locus_tag="AC16CX_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly ATPase TfpB"
                     /protein_id="HIR3012730.1"
                     /translation="MPTAKAAQPQADRRLELSQVLADLVADGLVPKETADQLVKNRRF
                     ARSDVHPLVVMADQKWKDPREPKKLLHLEALTQWLAGKVGLPYFHIDPFKIDFAAVTK
                     TMSSAYAERYHILPVAINTREVTIATCEPFVRDWEEQIKQVLRLEIKRVIANPVEIQS
                     YLIEFYNLARSVKGATEKDKGALSDIANFEQLVQLGRSGKLDANDQHVVHICDWLFNY
                     AFEERASDIHLEPRRDVGNVRFRIDGVLHQVYQIPTPVMAAMTSRIKVLGRMDVVEKR
                     RPQDGRLKTVTPDSQEVELRLSTMPTAFGEKLVMRIFNPENLVKDYKELGFTEEDERK
                     WTQMSNQPHGIILVTGPTGSGKTTTLYSTLKQLAVPEVNVCTVEDPIEMVEPTFNQMQ
                     VQHSIGVDFASGIRTLMRQDPDIIMVGEIRDRETAEMAIQAALTGHLVLSTLHTNDAP
                     SAVTRLLDLGVPSYLLQSTILGVMAQRLLRTLCPHCKEQHDLEDSAWEHMVSPWKAPK
                     PKGTTYIAKGCLECRMTGYMGRIGIYETLVVTPELKRAITDETDLAALQDKAYKEGMK
                     PLRINGAMKVAAGLTSVDEVTTVAPPPSGDRRQHPR"
     gene            2265..2603
                     /locus_tag="AC16CX_09230"
     CDS             2265..2603
                     /locus_tag="AC16CX_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012731.1"
                     /translation="MRTPLICFAAALLFLAAANAMARDRADTPPLRVFDAGELAPDRY
                     SVVKRLWTGTWRASFWVPGHDDAGAAVAALTQEAASLGAHGVVNLHCLNDAGGWGGGY
                     FCYGLAIKLK"
     gene            complement(2684..3739)
                     /locus_tag="AC16CX_09235"
     CDS             complement(2684..3739)
                     /locus_tag="AC16CX_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR3012732.1"
                     /translation="MTYIAKPKLHHPTLARNKVGYTRRDYEGAISTLCAGCGHDSISA
                     AIVQACWELDLEPHRVAKLSGIGCSSKTPTYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYMGVSGDGDSASIGLGQFAHSIRRGVHMVYIVENNGVYGLTKGQFSATADKGSKS
                     KRGVVNTDEPLDMIGLALLLGATFVARSFSGDKKQLVPLIKAAIQHRGAAFLDVISPC
                     VAFNNHPGSTKSYDYVREHNETVNRLDFIEGREEITADYAPGEVVEVAQHDGSMLRLR
                     KLAPDYDPTGRIKVMNYLQERSAAGEVVTGLLYVQPDSADLHQHLNTVETPLNQLTER
                     ELCPGSVMLERINAALR"
     gene            complement(3791..>4387)
                     /locus_tag="AC16CX_09240"
     CDS             complement(3791..>4387)
                     /locus_tag="AC16CX_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3012733.1"
                     /translation="LEAGKEFGRYLDVDGDGIPYRTYPGTHPTRGAYFTRGTTKDRYA
                     RYSEAGPDYVDNMQRLLRKFETAKQLVPKPLLRPAEKPARFGAIYYGSTSPAMQEALH
                     VLSSRGIHVNALRVRGFPFQDEIADFVASHPWVFVVEQNRDAQLKTLLVNEAKVNASR
                     LLSVLHYDGTPITARFIVDQISQHAAARMVVPLKKVGS"
BASE COUNT          706 a         1356 c         1449 g          876 t
ORIGIN      
        1 tccggcccag cgcgtgaaca ggcgctggaa atgatgctcg ctcaagcgca ggtatcgcgc
       61 caccgccgcg aggtccggtt ggtccgcggc gttccgcctg aggaattcga tcgcgcgcgc
      121 gattcgctcg taatccctta cgccggatcc gcgagtcggc atatcctcca atggagtatc
      181 gacaggtttc atgcacgcca gaatacggcg cgcatgaatc ttccgccacc cgattcttgc
      241 ttaaatgctg gtgagcggcg agcagtaagc ggtaagcgga taaagcgttg actgctcact
      301 gcttaccgct gaccgctgac agctatctcg gatgctgcct gcggtcgccg ctcggaggcg
      361 gggcgacggt ggtgacttcg tcgacgctgg tgaggcccgc tgcgaccttc atcgcgccgt
      421 tgattcgcag cggcttcatg ccctccttgt aggccttgtc ctgcagcgcc gcaaggtcgg
      481 tctcgtccgt gatggccctt ttcagctcgg gcgtgaccac cagggtctcg tagatgccga
      541 tgcggcccat atagcccgtc atgcggcatt cgagacagcc cttggcgata tatgtggtgc
      601 ctttgggttt gggcgccttc cagggggaca ccatgtgctc ccaggcggaa tcctcgaggt
      661 cgtgctgttc cttgcagtgg gggcagagcg tgcgcagcag ccgctgcgcc attacgccga
      721 ggatggtgga ttgcaagagg taagagggca caccgaggtc gagcagccgc gtgaccgccg
      781 agggtgcatc gttggtgtgc agggtggaaa gcacgagatg ccccgtgagc gccgcctgga
      841 tcgccatctc ggcggtctcg cggtcgcgga tctcgcccac catgatgatg tccgggtcct
      901 ggcgcatcag cgtgcggatg ccgctcgcga agtccacccc gatcgagtgc tgcacctgca
      961 tctggttgaa agtgggctca accatctcga tcgggtcctc cacggtgcag acgttcactt
     1021 ccggcaccgc cagctgcttg agcgtcgaat agagggtcgt ggtcttgccc gagccggtgg
     1081 ggccggtcac caggatgatg ccgtgcggct ggttcgacat ctgcgtccac ttgcgctcgt
     1141 cctcttctgt gaagccgagc tccttgtagt ccttcaccag gttctcggga ttgaaaatcc
     1201 gcatcacgag cttctcgccg aacgcggtgg gcatggtgga gagccgcagc tccacctcct
     1261 gactgtccgg ggtgacggtc ttcagccgcc cgtcctgcgg gcggcgcttc tccacgacgt
     1321 ccatgcgccc cagcaccttg atgcggctcg tcatggccgc catcaccggc gtcgggatct
     1381 ggtagacctg gtgcagcacg ccgtcgatac ggaaacgcac gttgcccaca tcgcgcctgg
     1441 gctcgagatg gatgtcgctc gcgcgctcct cgaacgcgta attgaacagc cagtcgcaga
     1501 tatggacgac gtgctggtcg ttggcgtcga gcttgccgct gcgcccgagc tgcaccagtt
     1561 gctcgaagtt ggcgatatcg ctgagcgcgc ccttgtcctt ctcggtcgcg cccttgaccg
     1621 agcgcgccag attgtagaac tcgatcagat aactctggat ctcgaccgga ttggcgatga
     1681 cgcgcttgat ctcgagccgc agcacctgct tgatctgctc ttcccagtcg cgcacgaacg
     1741 gctcgcaggt ggcgatggtg acctcgcgcg tgttgatggc gaccggcagg atgtggtaac
     1801 gctcggcgta ggcgctcgac atcgtcttgg tcaccgcggc gaaatcgatc ttgaacggat
     1861 cgatgtggaa atagggcagc ccgaccttgc ccgccagcca ctgggtgagc gcctcgaggt
     1921 gcagcagttt tttcggctcg cgcgggtcct tccacttctg gtcggccatc accaccagcg
     1981 ggtgcacgtc gctgcgcgcg aaccgccggt tcttgacgag ctgatcggcg gtttccttgg
     2041 gcaccagccc gtcggccacc agatcggcga gcacctgtga caactcgagg cgccgatcgg
     2101 cctggggctg ggcggctttt gcggtgggca tgggctggga acgggaattg tgcgtgacta
     2161 taaccgagac atgtaattca ctcaatgggt tacatcgtat atccattgct ggacatcccg
     2221 tatggctata atccgctttg cgacaccgga gacgggagag gaacatgcgc acgccgctga
     2281 tctgctttgc cgccgcgctg ctcttccttg cagccgcaaa cgccatggcc cgggaccggg
     2341 cggatacccc accgctcagg gtgttcgatg cgggagagct ggcgcccgat cgctactcgg
     2401 tggtgaagcg gttgtggacg ggcacctggc gcgcgtcgtt ctgggtccct ggccacgatg
     2461 acgcgggtgc ggcagttgcc gcgctcaccc aggaggcggc cagcctcggc gcccacggcg
     2521 tcgtcaatct gcattgcctg aacgatgccg gcggctgggg tggcggctac ttctgctacg
     2581 gcctggcaat aaagcttaaa tagctgtcag tcgtcagcca ctaccgcaag ctaagtttta
     2641 gctgatcgct gatagccggt gacgggacgc agttcgcgga ctgtcaccgc aacgcggcgt
     2701 tgatgcgctc cagcatcacg ctgcccgggc acagttcccg ttcggtgagc tggttgaggg
     2761 gggtctccac ggtattgagg tgctggtgaa ggtcggccga gtcgggctgg acgtagagca
     2821 ggccggtcac cacctcgccc gccgcggagc gctcctgcag gtagttcatc accttgatgc
     2881 ggccggtcgg atcgtaatcc ggcgcgagtt tgcgcagcct cagcatcgat ccgtcgtgct
     2941 gtgccacctc gacgacctcg cccggcgcgt aatccgcggt gatctcttcc cggccttcga
     3001 tgaaatcgag ccggttcacg gtctcattgt gctcgcgcac gtaatcgtag ctcttggtcg
     3061 aacctggatg gttgttgaac gcgacgcacg gcgagatcac gtccaggaaa gccgcgccgc
     3121 ggtgctggat cgcggccttg atgagcggca cgagctgctt cttgtcaccc gagaaactgc
     3181 gcgcgacgaa ggtggcgccc agcagcagcg ccaatccgat catgtcgagc ggttcgtcgg
     3241 tattgacgac gccgcgcttt gacttcgatc ccttgtcggc agtggccgag aactggccct
     3301 tggtgaggcc gtagacaccg ttgttctcga cgatatagac catgtgtacg ccgcgccgga
     3361 tgctgtgcgc gaactgcccg aggccgatcg aggccgagtc accgtcgccc gacacgccca
     3421 tgtagatgag atcacggttg gcgagattgg cgccggtcag caccgatggc atccgtccgt
     3481 gcacgctgtt gaagccgtgc gagtttccga gaaaataggt cggggtcttg gacgagcagc
     3541 cgatgcctga gagcttggcg acgcggtgcg gctcgaggtc gagctcccag cacgcctgca
     3601 cgattgccgc ggagatcgag tcgtggccgc agccggcgca cagggtcgag atcgcgccct
     3661 cgtagtcgcg acgggtgtag ccgaccttgt tcctggccag cgtcgggtga tgcagcttcg
     3721 gtttcgcgat atacgtcatg gcttcgcccg ataaatcgtt atttgtgatt cgtgaatcgt
     3781 gacagcagtg tcatgacccc accttcttca gcggaaccac catccgcgct gccgcgtgct
     3841 gcgagatctg gtccacgatg aagcgcgcgg tgatcggcgt gccatcgtag tgcagcaccg
     3901 agagcaggcg cgaggcgttc accttggcct cgttgacgag cagcgtcttc agctgcgcgt
     3961 cgcggttctg ctcgaccacg aacacccacg ggtgcgaggc gacgaagtcc gcgatctcgt
     4021 cctggaacgg gaagccgcgc acgcgcagcg cgttcacgtg gatgccgcgt gaggacagca
     4081 catggagcgc ttcctgcatt gcggggctgg tcgagccgta atagatcgcg ccgaagcggg
     4141 ccggcttctc ggcgggccgc agcaggggct tcggcaccag ctgcttcgcg gtctcgaact
     4201 tgcgcagcag ccgctgcatg ttatcgacgt aatcggggcc ggcctcgctg tagcgcgcgt
     4261 agcggtcctt ggtggtgccg cgcgtgaagt aggcgccgcg cgtcgggtgc gttcccgggt
     4321 aggtgcggta cgggatgccg tcgccgtcca cgtcgaggta gcggccgaat tccttgcccg
     4381 cctcgag
//
LOCUS       DBNGWZ010000320         5446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_542049, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000320 DBNGWZ010000000
VERSION     DBNGWZ010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_542049"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="AC16CX_09245"
     CDS             complement(<1..510)
                     /locus_tag="AC16CX_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3012734.1"
                     /translation="MNSLEKMLSLLDVFTPAAPAWSTDELIRYSGVSRSTCYRYIKVL
                     QRAGFLTPVASGSYILGPRIIELDRQIRMCDPVYTAGGGPMQQLARQTGHSALLCMLY
                     SDTVMCVREELNADAPEGLFSRGQKRPLFAGAASKVILAHLPAHQLRSLFAKHRKTIA
                     AAGLGADWES"
     gene            786..1754
                     /locus_tag="AC16CX_09250"
     CDS             786..1754
                     /locus_tag="AC16CX_09250"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012735.1"
                     /translation="MIRIWVLAFVFSSLDVVSHAQTTQVWPARPIRIIVPFQPGAFTD
                     VSARTLAVELTGQLGQQVIVENRTGAGGTIGTEMAARATPDGYTLLLSDNSFAISPGL
                     YAKLPYDPLKDFIHVSQIAESPSLMSGRIKLPAKSLKELVSLARANPGKLTFGSGGQG
                     SSAHLAMELFLNVAGIKMLHVPFKGIGPALAEAVADRIDVLISSLAAGAPHIHNERTR
                     GYAVSGKERHPILPGVPTFTEAGFPRYDMSYWWGIAAPVGTPREVVTRLNQEITRAAE
                     KPSLKGVFQKQGAVAVTSSPADHTRRVNAEIQTWKEVIARAGVKLE"
     gene            complement(1868..2755)
                     /locus_tag="AC16CX_09255"
     CDS             complement(1868..2755)
                     /locus_tag="AC16CX_09255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012736.1"
                     /translation="MKIGIAGTGRMGAAIAQRLMGLGHELTVWNRTPEKTKELAASGA
                     KVAASPASLVGGTEAIITILTDDKAIDALYSGAQGLLSGNVKGKLFIEMSTVRPEVEI
                     ALAAKVRQKHAAMVECPVGGTTGPAREGKLFGFAGGEDGDVARARPLLEQMCRRVEHC
                     GPVGAGTRMKLAINLPLFVYWQALGEALLLCRSLNLDPDRLMSILADTSGAPNALKTR
                     GAAVAAHLAGKDAGPVNFAVDLVCKDLRTMIAEARALGGSLPAAEAALKCFEEAARSG
                     LGEKDQSALPANWAKRRNR"
     gene            2997..4712
                     /locus_tag="AC16CX_09260"
     CDS             2997..4712
                     /locus_tag="AC16CX_09260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3012737.1"
                     /translation="MHIDWDAPIRMDDGLVLRADVFRAPRDGRFPVILSYGPYAKGLA
                     FQDGYPSAWQRMVAEHPDVAYGSSNRHQSWEVADPEKWVPHGYACVRVDSRGAGRSPG
                     FIDPFSPRETRDLCNCIEWAGVQPWSNGKVGLNGISYYGMNQWHVATLQPPHLAAMCI
                     WEGSADWYRDMTHHGGILSSFWGNWYDMQVKTVQYGLGERGPRSRVTGELVCGDETLS
                     DSELAKNRCDFGDNILAHPLDDDYHRARSPIWEKIITPFLSAANWGGQGLHPRGNFEG
                     FVRAASKEKWLEAHGLEHWTHFYTDYGRLLQKRFFDYFLKGEKNGWDKQPRVQLQIRH
                     ADRFVERQENEWPIARTQWTKFHLHPTAHKLDPEPTSAAAGITYEGPGEGVTFISAPL
                     DKETEITGPLASKLFISSSTTDADLFLVFRVFTPDLREVVFMGAIDPHTPIAQGWLRA
                     SHRKLDPRLSLPYRPYHTHDEKRPLKPGEIVELDVEIWPTSIVVPAGHRIALTVRGKD
                     YEWQKTTGARLSNFKNELRGCGPFLHDDPRDRPATVFGGSVTLHAGPQYPSYILLPII
                     PPKNT"
     gene            4809..>5446
                     /gene="maiA"
                     /locus_tag="AC16CX_09265"
     CDS             4809..>5446
                     /gene="maiA"
                     /locus_tag="AC16CX_09265"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HIR3012738.1"
                     /translation="MKYYGFWRSLAACRVRIALALKGIKAEEISVNLMQGEQLKPEYR
                     AVNPQAVVPALILDDGSTLFESLAIIEYLDEIHPQPPLLPRDPKGRARARGLAQIVAC
                     DGHPLIVPRIRNYLEKELNIDEAARTRWCQHWVMEALKFVESHLAGEKETGRYCHGDS
                     PTLADICVATQVFGARFFNCDTAPVPTVMRVFNECMKLEAFDKSHPLKQPGAP"
BASE COUNT         1004 a         1837 c         1648 g          957 t
ORIGIN      
        1 gctctcccag tcggcgccaa ggccggcagc cgcaatggtt ttgcggtgct tggcgaacag
       61 gctgcgcagc tggtgggccg gcaggtgcgc gagtatcacc ttggaggccg ctccggcgaa
      121 taacgggcgt ttctggcccc ggctgaaaag accttccggc gcatccgcat tgagttcctc
      181 acgcacgcac atcaccgtgt cgctgtaaag catgcacagg agcgcgctgt gcccggtctg
      241 ccttgcaagc tgctgcatcg gcccgccgcc ggccgtatac acgggatcgc acatccggat
      301 ctgccggtcc agctcgatga tccggggccc cagaatatag gatccgctgg cgacgggagt
      361 cagaaatccg gcgcgctgca gcaccttgat gtagcgatag cacgtcgagc gcgagacccc
      421 ggaataccgg atcagttcat ccgtggacca cgctggcgcc gcgggcgtga acacgtccag
      481 caggctcagc atcttctcga ggctgttcat ggaccggcct cgggggacag cggcaccctc
      541 acgtcgacca cggctggatt ctggacgtct tgccacgatt atccctggtt gctccggcaa
      601 ccgacctcgc atgcatccgc aattgaaaag tatctcacta ttctggacat tattatgcca
      661 cggcgctacc atgccttcac caatctcagg cggcgcggca cgccaccgag aagtgcagcg
      721 tgcccgggcc gcgggcgact atgtcccctg atgctgggat gaacccccga taactggaga
      781 ctccgatgat caggatatgg gtccttgcat tcgttttttc ttcgctggac gtggtatcgc
      841 acgcacaaac tacgcaggtc tggccagcca ggccgatccg catcatcgtg cctttccagc
      901 ccggggcgtt caccgatgtg tccgcgcgca cgctggctgt cgagcttaca ggccaactcg
      961 gacaacaagt catcgtcgag aaccgcaccg gggccggcgg cacgatcggc accgaaatgg
     1021 cggcccgcgc gacgcccgac ggctacaccc tgctgctctc ggataactcc ttcgcgatct
     1081 ctccgggcct gtatgccaag ctgccctacg acccgctgaa ggacttcatc cacgtcagcc
     1141 agatcgcgga atcgccttca ttgatgtcgg gacggatcaa gctgcccgcg aaatcgctca
     1201 aggagctcgt gagtcttgcc cgtgccaacc ccggaaaact gaccttcggt tccggcggcc
     1261 agggttcgtc ggcccatctc gccatggagt tgttcctcaa cgtggccggc atcaagatgc
     1321 tgcacgtccc attcaagggc atcgggccgg cgctggctga ggcggtggcg gaccgcatcg
     1381 acgtcttgat ctcgagcttg gccgctgggg cgccacatat ccacaacgaa cggacgcggg
     1441 gctacgccgt atccggcaag gaacgccatc cgatactgcc gggcgtgcca acgttcaccg
     1501 aagccggttt cccgcgttac gacatgtcgt actggtgggg catcgcggcg cccgtcggaa
     1561 cgccacgcga agtcgtgacc cggctgaacc aggaaatcac gcgcgccgcg gaaaagccat
     1621 cgctgaaggg cgtcttccag aagcagggcg ccgtcgcggt gacgagctcg cccgccgatc
     1681 acacccgccg cgtcaacgcg gagatccaga cttggaagga ggtcatcgcc cgagccgggg
     1741 tcaagctgga atgaagtatg caaaaaaacc cggcatccac tggccttttt tgctggaagg
     1801 cgtgggtcgt gactcgtgga acgtgattcg agcttttttc tcgcgaccgc gtcactgcgt
     1861 cacttcgtca ccgattccgg cgcttcgccc aattcgccgg cagcgcggac tgatccttct
     1921 ccccgagacc gctgcgcgcc gcttcctcga agcacttgag tgcggcctcc gccgccggca
     1981 gcgacccgcc cagcgccctc gcctcggcga tcatcgtccg caggtccttg cacaccaggt
     2041 ccacggcgaa gttcaccggg ccggcgtcct tgcccgcgag gtgcgctgcg accgcggcgc
     2101 cgcgcgtttt gagcgcgttc ggggccccgg aagtgtcggc gaggatgctc atcagccggt
     2161 cgggatccag attgagcgag cggcatagca atagcgcctc gccgagcgcc tgccaataca
     2221 cgaacagcgg caggttgatc gcaagcttca tgcgcgtgcc cgcgccgacc gggccgcagt
     2281 gctccacccg ccggcacatc tgttcgagca gcggcctggc ccgcgctacg tctccatcct
     2341 cgccgcccgc gaatccgaaa agcttccctt ccctggccgg accggtggtc ccgccgaccg
     2401 ggcattcgac cattgccgcg tgcttctgcc tcaccttcgc agcaagcgcg atctcgactt
     2461 ccggccggac cgtgctcatc tcgatgaaaa gcttgccttt gacgttgccg gagagcaatc
     2521 cttgcgctcc gctgtagagc gcatcgattg ccttgtcgtc ggtcagaata gtgatgatgg
     2581 cctcagtacc accgacgagt gacgcgggcg atgcggcaac cttggcgccg gaggcggcca
     2641 gctccttcgt cttctccggc gtgcggttcc acaccgtgag ttcgtggccg agtcccatca
     2701 gccgttgtgc gatggcggca cccatccggc cggtgcccgc gatcccgatt ttcatgtaac
     2761 gctccttttt atgggggcgc aggttgacac gggagacggc ggctcgtcaa actctccgcc
     2821 tcatgaagcg tgacaagtga taggtgatac gtgataagcg tcaagcgaca cgacaagtga
     2881 tccggctatg ccgggagacc tatcacttat cacttatcac cccccacggt tctccaatat
     2941 tgaccacgaa tcgtcctgcc atgagttcgc cggaaaaaag tgagattcgg gacggcatgc
     3001 atatcgactg ggacgccccg atcaggatgg acgacggcct cgtcctgcgt gccgacgtgt
     3061 tccgcgcgcc gcgcgatggc cgcttccccg tgatcctcag ctacggcccc tacgccaaag
     3121 gcctcgcctt ccaggacggc tacccgagcg cgtggcagcg catggtcgcc gagcaccccg
     3181 acgtcgccta tggttcgagc aaccgccacc agagctggga ggtcgcggac ccggaaaagt
     3241 gggtgcctca cggctacgcc tgcgtacggg tggactcgcg cggcgccggc cgctctcccg
     3301 gattcataga cccgttctct ccacgcgaga ccagggacct ctgcaactgc atcgaatggg
     3361 cgggggtgca gccgtggtcg aacggcaagg tcgggctgaa cggcatctcc tactacggca
     3421 tgaaccagtg gcacgtcgcg acgctgcagc cgccccatct cgccgccatg tgcatctggg
     3481 aaggctcggc cgattggtac cgcgacatga cgcaccacgg cggcatcttg agctcgttct
     3541 ggggcaactg gtacgacatg caggtgaaga cggtccagta cgggcttggc gaacgcgggc
     3601 cgcgctcgcg cgtcaccggc gagctggtgt gcggagacga gacgctctcc gactccgagc
     3661 tggcgaagaa ccgctgcgac ttcggagaca acatcctcgc gcatccgctc gatgacgact
     3721 atcaccgcgc ccgctctccg atctgggaaa agatcatcac gccgttcctg tcggcggcga
     3781 actggggcgg acagggcctg cacccgcgcg gcaacttcga ggggttcgtg cgcgccgctt
     3841 cgaaggaaaa atggctcgag gcgcacggcc tggagcactg gacccacttc tataccgact
     3901 acgggcggct cctgcagaag cgcttcttcg attatttcct gaaaggcgag aaaaacggct
     3961 gggacaagca gccacgggtg cagctccaga tacgccacgc ggatcgcttc gtcgaacgcc
     4021 aagagaacga gtggccgatc gcgcgcacac aatggacgaa gttccacctt catccaacgg
     4081 cgcacaaact ggaccctgag ccaacgagtg ccgcagccgg tattacctat gaaggaccgg
     4141 gagaaggtgt cacttttata tcagcgccgt tggataagga aaccgagatc acgggcccgc
     4201 tggcatcgaa actgttcatc tcctcctcca ccaccgatgc cgacctgttt ctcgtctttc
     4261 gcgtcttcac gccggacctg cgggaggtcg tgttcatggg cgcgatcgac cctcacacgc
     4321 cgatcgcgca gggctggctg cgcgcctcgc atcgcaagct cgatcccagg ctctcgctcc
     4381 cctaccggcc gtaccacacc cacgacgaga aacggccgct gaagccgggc gagatcgtcg
     4441 agctcgacgt cgagatctgg ccgacctcga tcgtcgtgcc tgccggtcac cgcattgcac
     4501 tgaccgtgcg cggcaaggac tacgaatggc agaagaccac cggcgcgcgg ctctccaact
     4561 tcaagaacga gctgcgcggc tgcgggccgt tcctgcacga cgatccccgc gaccgtcccg
     4621 ccacggtgtt cggcggtagc gtcacgctcc acgccggccc gcaatatccg tcgtatatac
     4681 tgctgcccat cattcccccc aaaaacacgt aagagcgtga gggcgggaga gcgacgaaga
     4741 ctggcttgtt gtttcgctct cacgacctcc cgatttcccg atcttccgga tcctgaaagg
     4801 aggagaaaat gaagtactac ggcttctggc gctcgctcgc cgcctgccgc gtgcgcatcg
     4861 cgctcgcgct gaaggggatc aaggcggagg agatttcggt caacctgatg cagggcgagc
     4921 agctcaaacc cgagtaccgc gcggtcaacc cgcaggccgt cgtgcctgcg ctcatcctgg
     4981 acgatggatc aaccctgttc gagtccctgg cgatcatcga gtacctggac gagatccatc
     5041 cgcaaccgcc gctgctgccc agggacccga agggccgcgc gcgcgcgcgc gggctggcgc
     5101 agatcgtcgc ctgcgacggc catcccctga tcgtcccgcg catacgcaac tacctcgaga
     5161 aagagctgaa catcgacgaa gccgcacgta cccgctggtg ccagcactgg gtgatggagg
     5221 cgctgaagtt cgtcgagtcc cacctcgccg gggaaaagga aaccggccgt tactgccatg
     5281 gcgattcccc caccctcgcc gacatctgcg tcgcgaccca ggtattcggc gcccgtttct
     5341 tcaattgcga caccgcgccg gtcccgaccg tgatgcgggt attcaacgag tgcatgaagc
     5401 tcgaagcctt cgacaagtcg cacccgctca agcagccggg agcgcc
//
LOCUS       DBNGWZ010000321        12761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1675490, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000321 DBNGWZ010000000
VERSION     DBNGWZ010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12761
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1675490"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..225
                     /locus_tag="AC16CX_09270"
                     /pseudo
     CDS             106..225
                     /locus_tag="AC16CX_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            298..1281
                     /locus_tag="AC16CX_09275"
     CDS             298..1281
                     /locus_tag="AC16CX_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012739.1"
                     /translation="MEQRILQTRAHLREHSVLGEYGADAIERAMHEELPDLLVSRATI
                     NRVLKHHGATDARARVRRPAPPKGWYLPEVAAGRAEVDCFDFIEDLRIGGGPLVNVLT
                     AKSLHGALTDAWVLAQLSAKGCVPCLLGRWRHDGLPAHAQYDNDTVFQGAHQFANAVG
                     RISRLCLALGVVPVFVPPLEHGMQNTIEGFNGLWQAKVWQRHHVGSVSELQARSDAYI
                     AAHRARSRQAAEAAPPRRPMPKHFELNLHAPLRGQIIFIRRTDESGHVHLLGQRFAVS
                     PEWPHRLVRCEVDFDHHCIRCFALRRHAPTEQPLLATLEYHRPNKPFKGEL"
     gene            complement(1385..2797)
                     /locus_tag="AC16CX_09280"
     CDS             complement(1385..2797)
                     /locus_tag="AC16CX_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3012740.1"
                     /translation="MPELWRLSATEAVALLKRREVSPLELIDAAAARIEATNPKINAF
                     VTLCYDRAREHARRLEKEPAGEVPPHYLYGLPIGVKDGTDVAGVRCTSGSRIYANRIA
                     PSSDIVVERLEANGAIVIGKTNLPEFAAGGNTFNDVFGATLNPWDTRMTASGSSGGSA
                     AALAAGQVWLATGGDFGGSIRTPSSYCSTTGLRPSAGMVPRTSKQPFSPLSVEGPMAR
                     NVADTALMFDCEAGWHPLDPLSQVGPHPSFSAAAARPRKPRRIAFSTDLGVARAVDRE
                     VRRVCKAAADRLSRDGVTVEEAHPDLADAEKTFLTLRGAVFIARHAQLIEKHRRLYKP
                     EIIENTEFGLRLKPEEIVAAEVAQGELIRRTAKFFENYDLLLCPVVTCPPFDVNVRYP
                     EQVDGVRFEGYMGWLILTYAITVTACPVVALPGGFTGAGLPVGLQLVGPPRSEARLIS
                     LAAYLESLLDVAPKTPIDPR"
     gene            2983..4674
                     /locus_tag="AC16CX_09285"
     CDS             2983..4674
                     /locus_tag="AC16CX_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012741.1"
                     /translation="MKPSETALARTGGQLLVDALKVHGVDLAFGVPGESFLEVLDALH
                     DARGQIQFIICRQESGAANMAEAYGKLTGRPGIYMVTRAPGASNAAVGMHTAFQDSTP
                     LILFIGQVGNDFVEREAFQEIDYRRMYGQMAKWVAQIDRADRVPEYVSRAFHIAVSGR
                     PGPVVLALPEDMLTATATVADTRRYQRIAAHPGEADMEKLRGMLAQAKHPFVILGGSG
                     WNAQACADFRAFVEANDLPVGTAFRRQDLYDNRQPNFVGDVGIGVSPALADRIKGADL
                     LLAIGARLDEMTTSGYTLIDAPRPKQKFVHVHAGAEELGRVYEPDLAIHSGMPEFSAA
                     VQKLAPVDARTWKEWTKAARADYEAWLRPGEMPGDFDLGQAMVFLRRRLPPETIVANG
                     AGNFSAWIHRFYQYTGIRTELAPTSGAMGYGVPAAVAAKLVHPDRPVVCFSGDGDFLM
                     TGQELATAAQYDLKIIFMVINNGMYGTIRMHQEREHPGRVSGTELKNPDFAALARAYG
                     LHGEIVEKTEDFEAAFERAWNAKTASLLELRIDPEAITTRTTLSAIRANALKSKK"
     gene            4753..5712
                     /locus_tag="AC16CX_09290"
     CDS             4753..5712
                     /locus_tag="AC16CX_09290"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3012742.1"
                     /translation="MIRYLTESDVQKLLTMPLALEAMEEAHRALAEHRAIDVPRARIH
                     LPTGTQHVLQAAVPELGMIGFKYYYTRPTGKSFYVHLINTETAKLEAIIEAVWMSMLR
                     TGAASGVATKHLARPDAGVVGQIGAGYQGTSQLEAVCAVRKIHTAKVFARNRDRLVAF
                     CNQMSQKIGIPVQPAESAEAAVRGAHIVNVITKSAEPVLKGAWLEPGQHVNAAGSNAL
                     TRREIDDAAVKKCDVITVDGRGTARNECGDLLPAVEKGWLRWDYLTEIGEVIAGKAPG
                     RTSAKQVTLYESHGMGLQDVYVGAKVLELARSRGVGTELPVGL"
     gene            complement(5888..6349)
                     /gene="sixA"
                     /locus_tag="AC16CX_09295"
     CDS             complement(5888..6349)
                     /gene="sixA"
                     /locus_tag="AC16CX_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR3012743.1"
                     /translation="MRVYLVQHGEAQPEEVSLERELTPRGCSDVERAAALLGRCGVRV
                     ARVYHSGKTRARQTAELLAERVAPGITPEVLAGINPNDPVGPFAERMRDWSDDTLVAG
                     HMPFMGRLAALLVTGRDQPPVVAFKQGSVACLERDVEGHWAVAWMVRPELL"
     gene            6568..7413
                     /locus_tag="AC16CX_09300"
     CDS             6568..7413
                     /locus_tag="AC16CX_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase"
                     /protein_id="HIR3012744.1"
                     /translation="MARPLRLEFEGAIYHVTSRGDRCEPIFQDDSDRNMLLAVVKKAM
                     ERFDAVVFAYCLMENHYHIVLQTRRPNLSRLMRHVNGVYTQAYNRRHAQVGHLFQGRF
                     NAVLVDKDAYFLEVCRYVDLNPVRAGRVSQPGDWAWSSYRAHTGQVLAPNWLDSSALH
                     QQLSGRLDKGPERYARFVAAGRGVRLWDEALSGQIYLGNAQFVRRMQMRAGFVHDREI
                     PKAQRRPKARPLSWYLEHHDRDTAIAMAYRDGGHTQSAIAGATGLSISRVSRLISSKE
                     AKGKT"
     gene            complement(7425..8261)
                     /gene="panC"
                     /locus_tag="AC16CX_09305"
     CDS             complement(7425..8261)
                     /gene="panC"
                     /locus_tag="AC16CX_09305"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3012745.1"
                     /translation="MDVIHSAADLRARLQREPNNVFVPTMGNLHDGHIQLINIAKPRA
                     ACTVVSIFVNRLQFGPREDFDRYPRTLEADCARLKAAGVDVVFAPDEKEIYPEPQTFV
                     VEPSDLQYILEGAHRPGHFRGVATVVLKLFSLVMPRSAIFGKKDYQQYLVLRDMVRQF
                     ALPIEIIPAETVRAPDGLALSTRNVYLSPEERKEAPRLYSVLKKARAEIEGGARDFQR
                     IDQQAMAELTRHGWQPDYVAVRRQADLRPPTERDRDLVVVAAARLGRTRLIDNVEINL
                     PR"
     gene            complement(8371..9168)
                     /gene="panB"
                     /locus_tag="AC16CX_09310"
     CDS             complement(8371..9168)
                     /gene="panB"
                     /locus_tag="AC16CX_09310"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3012746.1"
                     /translation="MRVTLSTLQKMAQEGSKIAMLTCYDASFATVLEEAGVDSLLIGD
                     SLGNVLQGHESTLPVTLRDMVYHTQCVARGSKRAFIIGDMPFGSFQVSPEETLRNAAE
                     IMAAGAHMVKIEGGRPMLETIEFLTDRGIPVCGHLGLTPQSVHQLGGYKVQGKGESEA
                     HRLLREAKQLEQAGAGMVVLEAIPATLARDVTAAITIPTIGIGAGVDCSGQVLVLYDL
                     LDVFPGKKAKFVKNYMRPAGSIQGAVELYVKEVKAKAFPGPEHSFSA"
     gene            complement(9285..9929)
                     /locus_tag="AC16CX_09315"
     CDS             complement(9285..9929)
                     /locus_tag="AC16CX_09315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR3012747.1"
                     /translation="MTKLPDKYRYVVVEGPIGAGKTSLARVMSERTGAAALLEDPDAN
                     PFLPRFYQDRARYALPTQLYFLFQRAAQVRALSQPDLFAGVTIADFMIDKDSLFARLT
                     LNDDEFALYRQVYAQLKPRAPTPDLIIYLQASAETLIERVRRRGVAYERDIADEYLVR
                     LAETYARFFYQYNAAPVLIVNSENLNFVDQPGDFDLLLGRIAGMRGPREFFSFG"
     gene            complement(10024..10515)
                     /gene="folK"
                     /locus_tag="AC16CX_09320"
     CDS             complement(10024..10515)
                     /gene="folK"
                     /locus_tag="AC16CX_09320"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3012748.1"
                     /translation="MALGSNLDDPPAQIRSALRALAALPETRVVRQSSLYRNPPVGRL
                     DQPEFVNAVAEIGTRIGPRELLDRLLEIERTHGRVRDYPNAPRTLDLDILLYGDRVVH
                     EAGLTIPHPRMLERAFVLVPLAEIAPETVVPGKGRVADLVRSVDASVLMKLSDDDAAG
                     ECK"
     gene            complement(10590..11957)
                     /gene="pcnB"
                     /locus_tag="AC16CX_09325"
     CDS             complement(10590..11957)
                     /gene="pcnB"
                     /locus_tag="AC16CX_09325"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0043633 - polyadenylation-dependent RNA
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HIR3012749.1"
                     /translation="MIRRFLGRVFSGRLFGPAQPKIIPPNAHGIDRGAISSCALRTTQ
                     TLQRHDFAAFVVGGAVRDLILGRKPKDFDVATDATPEEVRELFRRSRIIGRRFRLVHV
                     MCGPETVEVSTFRGMNPVDETDDHVADEHGRILRDNVFGTQVQDATRRDFTINALFYD
                     PATGEVWDYHDGVADLKKRRLRMIGDPERRYREDPIRMLRAVRLAASRGLEIDAPTRR
                     PIHRLAGLLAHVPPSRLLDEMLKLLLSGHATEGMSQLHREGLHHGVLPLLDAAMGRAV
                     GERFITAALRNTDKRVLAGKPVSPGFVFAALLWPEVQAEYKVLAEKGEPPVPALYRAM
                     DHVMRTQAGKLAIPHRYGADMREIWAMQPRFLQRAGKRPFRLLEQPRFRAGYDFLRLR
                     CESGEIEPALGEWWERFQRAGESERAHMLVSGEEPRRRRRRRRRRPRGADGGPAHGAA
                     PAAEV"
     gene            complement(12112..12756)
                     /locus_tag="AC16CX_09330"
     CDS             complement(12112..12756)
                     /locus_tag="AC16CX_09330"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR3012750.1"
                     /translation="MFDLDNTLLAGDSDFEWAQFLIEQGVLDREVYEARNQRFFDEYK
                     AGTLDIHAFLDFQLKPLSRHPRAQLDAWHREFMALKIRPIVRAKARTLVERHRGDLRV
                     VITATNSFVTAPIAREFGIADLIATEPEQRDGEFTGAVSGIPCFREGKVARLDAWLAG
                     RGASLASFTESWFYSDSHNDLALLGRVTHPVAVDPDPELRSHAERAGWRIISLD"
BASE COUNT         2253 a         4168 c         4178 g         2162 t
ORIGIN      
        1 ggccaaggct cagcgttcgc cgaacacctg aggctcagcg ttcgccgaac acttgcgata
       61 gggtaaagga ttttggggtg agttagtcat ggcgaaaaaa gctccattga acgcaagcgt
      121 cgcgagatgg ttcggcgagt ccgagccggg tttggtttgc gtacggtggc cagacagttt
      181 ggggttagtg tgagtacggt gtcgttgtgg gttgcccgca gtgccgatgg acggctggat
      241 cggtgtgcgt ttaccgatca taagcgcggt cgcgcttgga atcgcatgca gccgtccgtg
      301 gagcagcgca ttctgcaaac ccgagcccac ctgcgcgagc atagtgtgct cggcgagtat
      361 ggggctgatg ccatcgagcg ggcaatgcac gaggaactgc ctgacctgct ggtgtcgcgg
      421 gccacgatca atcgcgtgct caagcaccat ggggcaaccg atgcgcgcgc gcgggtgcgc
      481 cgtcccgcgc cgccgaaggg ctggtatttg cccgaggtgg cggcaggaag agcggaggtg
      541 gactgcttcg actttatcga ggacctgagg atcggcggcg gtccgctggt gaacgtgctc
      601 acggccaaga gcttgcatgg ggcgctcacg gatgcctggg tgctggccca gctcagtgcc
      661 aagggttgtg tgccttgcct gctggggcgc tggcggcacg atgggctgcc agcccatgcc
      721 cagtacgaca acgacaccgt gtttcagggc gcgcaccagt tcgccaacgc ggtgggccgc
      781 atcagccgcc tgtgtctggc tttgggcgtc gttccggtgt tcgtgccgcc gctcgagcac
      841 ggcatgcaaa acaccatcga aggcttcaac ggcttgtggc aggctaaggt ctggcagcgt
      901 caccatgtcg gtagcgtgag cgaactgcag gcgcgttcgg atgcctacat cgccgcccac
      961 cgggctcgta gccgccaggc tgccgaagca gccccgccgc gccgaccgat gcccaagcac
     1021 tttgaactca acctgcacgc ccccctgcgc ggacagatca tcttcattcg ccgcaccgac
     1081 gagagcggcc acgtgcatct gctcgggcag cgcttcgccg taagccccga gtggccgcat
     1141 cgcttggtgc gctgcgaagt cgacttcgat catcattgca tccgctgctt cgccctgagg
     1201 cgccacgctc ccaccgagca gccgctgctt gctacccttg aatatcaccg acccaacaaa
     1261 cccttcaaag gtgaactatg agtgttcggc gaacgctgag ccccggtcac atcaacgtca
     1321 aaaaagtgtt cggcgaacgc tgagccgcgc cctatcattc gtcacccatc gcgcatttcc
     1381 gtcgtcagcg gggatcgatc ggcgtcttcg gcgccacgtc gaggagcgac tcgaggtagg
     1441 ccgccaggga aatgagccga gcctcgctgc gcggcggccc caccagttgc agcccgacgg
     1501 gaagcccggc tcccgtgaag ccgcccggaa gcgcgaccac cgggcaggcg gtgaccgtga
     1561 tggcgtaggt caggatcagc cagcccatgt agccctcgaa gcgcacgccg tccacctgct
     1621 cgggataacg cacgttgacg tcgaagggcg ggcaggtgac cacgggacac agcagcaggt
     1681 cgtagttctc gaagaatttc gccgtgcgcc ggatcaactc gccctgcgcg acctcggccg
     1741 cgacgatctc ctcgggcttg aggcgaagcc cgaattcggt gttctcgatg atctccggct
     1801 tgtagaggcg gcggtgcttc tcgatgagct gcgcgtggcg cgcgatgaag accgcgccgc
     1861 gcagggtgag aaacgtcttt tccgcatccg ccagatcggg atgcgcttcc tccaccgtca
     1921 cgccatcgcg ggaaagcctg tccgccgccg ccttgcacac ccggcgcacc tcgcggtcca
     1981 ccgcgcgcgc aacgccgagg tcggtgctga acgcgatgcg ccgtggtttg cgcggccgtg
     2041 ccgcggccgc cgagaaactc ggatgcggcc ccacctgtga caacggatcg agtggatgcc
     2101 accccgcctc gcaatcgaac atgagcgccg tgtcggcgac gttgcgcgcc atcggtccct
     2161 cgaccgacaa cgggctgaac ggctgcttgg acgtgcgcgg caccatgccc gcgctcggac
     2221 gcagcccggt ggtcgagcag tagctcgaag gcgtgcggat gctgccgccg aaatcaccgc
     2281 cggtcgccag ccatacctgt cccgctgcga gcgccgccgc cgagccgccg gaggagccgc
     2341 tcgcggtcat gcgcgtgtcc cacggattga gcgtggcgcc gaacacgtcg ttgaaggtgt
     2401 tgccgcccgc cgcgaactcg ggcaggttgg tcttgccgat cacgatcgcg ccgttcgctt
     2461 ccaggcgctc cacgacgatg tcggaggacg gcgcgatgcg gttcgcgtaa atgcgcgagc
     2521 cggacgtgca gcgcacgccc gcgacgtccg tgccgtcctt caccccgatc ggaagaccgt
     2581 aaagataatg gggcggcacc tcgccggccg gttctttctc cagccggcgc gcgtgctcgc
     2641 gcgcgcggtc gtagcacagc gtgacgaacg cgttgatctt cgggttggtc gcctcgatgc
     2701 gcgccgctgc tgcatctatc agctcgagcg gcgatacttc gcggcgcttg agcagcgcca
     2761 ccgcctctgt tgcggaaagt ctccacagtt ccggcattgg cacccctctc ccatcatcct
     2821 tcccggccgg cagcggccgg gtgccggatt ctaccccggc cagaacagcc gctgcgcccc
     2881 gtcccacacc agtttcgcgc cggccaggaa cgtcagcgcg tagcacacgc ggtagaacca
     2941 gttcgtgata gatttagcct cccgcacgtt accggtcctt ccatgaagcc ctctgaaacc
     3001 gcgctcgccc gcaccggcgg ccaacttctg gtggacgcgc tcaaggtcca tggagtggac
     3061 ctcgcgttcg gcgtgcccgg cgagagcttt ctcgaggtgc tcgacgcgct gcacgacgcg
     3121 cgcgggcaaa tccaattcat catctgccgc caggaaagcg gcgccgccaa catggcggaa
     3181 gcctacggca agctgaccgg ccgccccggc atctacatgg tgactcgcgc gcccggcgcg
     3241 agcaacgccg cggtcggcat gcacacggcg ttccaggatt cgactccgct catcctcttc
     3301 atcggccagg tgggcaacga cttcgtcgag cgcgaggcgt tccaggagat cgattaccgc
     3361 cgcatgtacg gccagatggc gaaatgggtc gcgcagatcg accgcgccga ccgcgttccc
     3421 gagtacgtga gccgcgcctt tcacatcgcg gtttcggggc ggcctgggcc ggtcgtgctc
     3481 gccctgccgg aggacatgct caccgccacc gccaccgtgg ccgacacccg gcgctaccag
     3541 cgcatcgctg cgcatcccgg cgaggccgac atggagaagc tgcgcgggat gctcgcgcag
     3601 gcaaagcacc cgttcgtgat cctcggcggc agcggctgga acgcgcaagc atgcgctgat
     3661 ttccgggcgt tcgtggaggc aaacgacctt ccggtcggca cggcattccg tcgccaggac
     3721 ctctacgaca accggcagcc caatttcgtc ggtgatgtcg gcatcggggt gagtcccgcg
     3781 cttgcggacc gcatcaaggg cgccgatctg ctgctggcga tcggggcgcg gctcgacgag
     3841 atgaccacca gcggctacac gctgatcgac gcgccgcgtc cgaagcagaa gttcgtgcac
     3901 gtgcacgccg gcgcggaaga gctgggacgg gtctacgagc ccgacctggc gattcattcc
     3961 ggcatgccgg aattctccgc ggctgtgcaa aagcttgctc cggtcgatgc gcgcacgtgg
     4021 aaagagtgga cgaaagcggc gcgcgccgat tacgaagcgt ggctccgacc cggcgagatg
     4081 ccgggcgatt tcgatctggg acaggcgatg gtgtttctgc gccggcggct tccgccggaa
     4141 accatcgtcg cgaacggcgc gggaaacttt tcggcctgga tccatcgttt ctaccagtac
     4201 accggcatcc ggaccgagct cgcgcccacc agcggcgcga tgggctacgg ggtgccggcc
     4261 gcggtcgccg cgaagctggt tcaccccgac cggccggtgg tgtgtttctc cggcgacggc
     4321 gatttcctga tgaccggcca ggaactcgcg accgcggcgc agtacgatct gaagatcatc
     4381 ttcatggtga tcaacaacgg catgtacggc acgatccgca tgcaccagga gcgcgagcac
     4441 ccggggcgcg tgagcggcac cgagctgaag aaccccgact tcgcggcgct tgcccgcgcc
     4501 tacgggttgc acggtgagat cgtcgagaag accgaggact tcgaagccgc gttcgagcgg
     4561 gcgtggaatg cgaagaccgc ctcgctcctc gagctgcgca tcgatcccga ggcgatcacc
     4621 acgcggacga ccctgtccgc cattcgggcg aatgctctca agagcaagaa gtgactcgtt
     4681 tctcgttggt ggcagcgctg agaaccccga atcacgagta acgaggaacg aagaacgagt
     4741 caccctctcc atatgatccg ctaccttacc gaaagcgacg tccagaagct gctgaccatg
     4801 cccctggcat tggaagcgat ggaggaagcg caccgggcgc tcgccgagca ccgcgcgatc
     4861 gacgtgccgc gggcgcgcat ccacctgccg accggcaccc agcacgtgct gcaggccgca
     4921 gtgccggagc tggggatgat cggcttcaag tactactaca cgcgtcccac gggcaagagc
     4981 ttctacgtcc atttgatcaa caccgagacc gcgaagctcg aggcgatcat cgaggcggtg
     5041 tggatgagca tgctgcgcac aggggcggcg agcggggtcg cgacgaagca tctcgccagg
     5101 ccggacgccg gcgtcgtcgg ccagatcggc gccggctacc agggcaccag ccagctcgaa
     5161 gccgtgtgcg ccgtcaggaa gatccacacg gcgaaagtgt ttgcgcgcaa ccgcgaccgg
     5221 ctggtcgcgt tctgcaacca gatgtcgcag aagatcggca tcccggtcca gccggcggag
     5281 agcgcggaag cggcggtgcg aggcgcgcat atcgtcaacg tcatcaccaa gtcggcggag
     5341 ccggtgctga aaggcgcgtg gctcgagccc gggcagcatg tcaacgccgc cggctccaac
     5401 gcgctcaccc ggcgcgagat cgacgatgcg gcggtcaaga agtgcgacgt aatcacggtg
     5461 gatggccgcg gcaccgcccg caacgaatgc ggcgacctgt tgcctgccgt cgagaagggg
     5521 tggctgcgct gggattatct gaccgagatc ggcgaagtca tcgccgggaa ggcgcccggc
     5581 cgcacgtcag cgaagcaggt cacgctctac gagtcccacg gcatgggatt gcaggacgtc
     5641 tacgtcggcg cgaaggtgct cgagctcgcc cgcagtcgcg gcgtcggcac cgagctgcct
     5701 gtcggccttt gaaccgccga gagagtcaaa atcaatttcg aaccacagag acacagagag
     5761 aaacaaaaac aataattgaa ccgccacgac gccaagagaa aacaaaaaat ggggtcggag
     5821 cctgaggtct cctcacaaat tacgcttgta cgtcagcggc ggcgggaaaa ggttcctgac
     5881 ccctttttta gagcagctcg ggccgcacca tccacgccac cgcccagtgg ccttccacgt
     5941 cccgttccag acaggcgacg ctgccctgct tgaacgccac cacgggaggc tggtcgcgcc
     6001 ctgtgacgag cagcgcggcg agccgcccca tgaacggcat atgccctgcc accaatgtgt
     6061 cgtcactcca gtcgcgcatc cgttccgcga aaggcccgac cggatcgttg ggattaatgc
     6121 cggcgagaac ctccggcgtg attcctggcg cgacgcgctc ggcgagaagt tccgcggtct
     6181 gccgggcacg ggtcttgcca ctgtggtaca ccctggcaac gcgcacgccg cagcgcccga
     6241 gcagtgccgc cgcccgctcg acatcgctgc aaccacgcgg cgtaagctcc cgctccaggc
     6301 tcacttcctc gggctgcgct tcaccgtgct gaaccaggta tacccgcata agacgctcag
     6361 cgtagagaca attctacatt cccaatcagt caaaaaatct ggcgagtgat ttcgactggt
     6421 gtcaaaaaac tcggaatcca ctgcggcccg gaaaccccac catggggtca ggtcttgcct
     6481 tttgcctgct ggccgggctg gtaagtgctg gcgtcagtca acgtcagggt caggtcttgc
     6541 gttttgccta cagatccgga gtgcacgatg gctcgtcctc tcagactgga gttcgaaggc
     6601 gcgatatatc acgtcacgtc gcgcggggac cgttgcgagc cgatttttca agacgattcc
     6661 gatcgcaata tgctgctggc cgtggtcaag aaggcgatgg aacgttttga cgccgtggta
     6721 tttgcctact gcttgatgga aaatcactat cacatcgtcc tgcagacacg ccggccgaat
     6781 ctgtctcggt tgatgcgtca cgtcaacggc gtttataccc aagcctataa ccggcgccat
     6841 gctcaagtcg ggcatttgtt tcaggggcgc ttcaacgcgg tgttggtcga taaggatgcg
     6901 tatttcctgg aagtctgccg ttacgtcgat ctcaatcccg tccgggccgg cagggtgagc
     6961 caacctggag actgggcgtg gagcagctac cgcgcccaca cagggcaggt tttggcgccg
     7021 aactggcttg acagttcagc gttgcaccaa caattgtccg ggcggttgga caaggggcca
     7081 gagcgctatg cccggttcgt tgccgcaggg cgaggggtga ggttgtggga cgaagcgttg
     7141 agcggccaga tctatctcgg gaacgcccag ttcgtacgca ggatgcaaat gcgcgctgga
     7201 ttcgtgcatg atcgcgagat tccaaaggcg caacgccgac ccaaggcgcg tccgctttcg
     7261 tggtatctgg aacaccatga tcgggatact gctatcgcaa tggcctaccg ggacggcggg
     7321 catacacaat cagcgatcgc cggcgccacc ggactgtcca tatcgcgagt cagtcggctg
     7381 atttcgtcga aggaggcaaa aggcaagacc tgaccccaag agggttagcg cggcaggttt
     7441 atctcgacgt tgtcgatgag ccgcgtgcgg ccgaggcgcg cggcggccac gacgacgagg
     7501 tcgcgatcgc gttccgtggg cggccgcaag tcggcctgcc ggcgcacggc gacgtaatcg
     7561 ggctgccaac cgtggcgcgt gagctcggcc atcgcctgtt gatcaatgcg ttggaaatcg
     7621 cgcgcgccac cctcgatctc cgcccgggcc ttctttagga cactgtagag gcgcggcgcc
     7681 tccttgcgtt cttcaggcga caggtagacg ttgcgcgtgg acagcgcgag gccatccggc
     7741 gcgcgcaccg tttcagcggg aatgatctcg atgggaagcg cgaactggcg caccatgtcg
     7801 cgcagcacga gatattgctg gtagtccttc ttgccgaaga ttgccgagcg cggcatgaca
     7861 agactgaaga gcttgagcac gaccgtcgcg actccccgga aatgccccgg ccggtgcgcc
     7921 ccctccagga tgtattgcag gtctgagggt tcgaccacga aggtttgcgg ctcggggtag
     7981 atttccttct cgtcgggggc gaacacgacg tccacgcccg ccgccttgag ccgcgcgcag
     8041 tcggcctcca gcgtgcgcgg atagcggtcg aaatcctcgc gcggcccgaa ttgcaggcgg
     8101 ttcacgaaga tgctcaccac ggtgcacgcc gctcgcggtt tggcgatgtt gatgagctgg
     8161 atgtgcccgt cgtgcaggtt gcccatagtc ggcacgaaca cgttgttggg ctcgcgttgc
     8221 aggcgcgccc gcagatcggc ggcggaatga attacgtcca tcggtcgcgc cgtcgcgctt
     8281 aaggatgaag gaacagcttc ccgcgacgcc ccgtcatgtc acgccgtatc acgtcacgca
     8341 gcaccacgtc acggttcatc acgtcacgct tcacgccgaa aacgagtgtt cgggcccggg
     8401 aaaggccttc gctttgactt ccttgacgta gagctcgacc gcgccctgga tcgagccggc
     8461 cgggcgcatg tagttcttga cgaatttcgc cttcttgccg ggaaagacgt cgagcaagtc
     8521 gtacagcacc agcacctggc ccgagcaatc gacgccggcg ccgatgccga tggtgggtat
     8581 cgtgatcgcc gcggtgacgt cgcgcgccag cgtggcggga atcgcctcga gcacgaccat
     8641 gccggcgccc gcctgctcga gctgcttcgc ctcccgcagc aggcgatgcg cctcgctctc
     8701 gcccttgccc tgcaccttgt agccgccgag ctggtgcact gactgcggcg tcagccccag
     8761 gtgcccgcac accgggatgc cgcggtcggt gaggaactcg atcgtttcca gcatcggcct
     8821 gccgccctcg atcttcacca tgtgggcgcc ggcggccatg atttcggcgg cgttgcgcag
     8881 ggtctcctcc ggcgacacct ggaagctgcc gaacggcatg tcgccgatga tgaatgcgcg
     8941 cttcgagccg cgcgccacgc actgggtgtg gtaaaccatg tcgcgcaggg tcaccgggag
     9001 cgtggactcg tggccctgca gcacgttgcc gagcgagtcg ccgatcagca gcgaatcgac
     9061 gcccgcctcc tcgagcacgg tggcgaagct cgcgtcgtag caggtcagca tcgcgatctt
     9121 gctgccttcc tgcgccatct tctgcagcgt gctgagcgtg actcgcatac aaatccccta
     9181 tcgacgcaaa gtcgcaaagg cgcaaagaat accgtaatca gggccaaacc caaacgaatc
     9241 cactcttgca ctctttgcgg cttcgcggct ttgcgtcaga tcgtttaccc gaagctgaag
     9301 aactcgcgcg ggccccgcat gcccgctatg cggcccaaca ataaatcaaa atcaccgggc
     9361 tgatcaacaa aattcagatt ctcggagttg acgatcagca cgggcgccgc gttgtactgg
     9421 tagaagaacc gcgcgtaggt ttccgcgagc cgcaccaggt actcgtcggc aatgtcgcgc
     9481 tcgtaggcga cgccgcgccg gcgcacgcgc tcgatcagcg tctcggccga ggcctgaagg
     9541 tagatgatca ggtccggcgt gggcgcccgc ggcttcaact gcgcgtagac ctggcgatag
     9601 agcgcgaact cgtcgtcgtt caacgtcagc cgcgcgaaca gcgaatcctt gtcgatcatg
     9661 aagtcggcga tggtcacgcc ggcgaacaga tcgggctggg acagcgcgcg cacctgcgcc
     9721 gcgcgctgga acaggaagta gagctgcgtc ggcagtgcgt agcgcgcgcg gtcctggtag
     9781 aagcgcggca ggaaggggtt ggcgtccgga tcctcgagca gcgcggcggc gcccgtgcgc
     9841 tcgctcatca cgcgcgcgag gctcgtcttg ccggcaccga tcgggccttc gacgacgaca
     9901 tagcggtact tgtcgggcag ctttgtcatg gaggagcgac cagcggtcag caatcagctg
     9961 tcagctacga gtttgcaacg atgtaacgct ctggctgaat gctgaaagct gatagctgaa
    10021 cactcatttg cattctcccg cggcatcatc atcagaaagc ttcatcaaaa ccgaggcgtc
    10081 cacgctccgc acgaggtcgg cgacgcgccc tttgcccggc accacggtct ccggcgcgat
    10141 ctccgcgagc ggcaccagga cgaaggcgcg ctccagcatg cgcggatgcg gaatggtgag
    10201 gcccgcctcg tgcaccaccc ggtcgccgta aagcaggatg tccagatcga gcgtgcgcgg
    10261 cgcgttgggg tagtcgcgca cgcggccgtg ggtgcgctcg atctcgagca gccggtcgag
    10321 cagctcgcgc gggccgatgc gggtcccgat ttccgccacg gcattcacga actccggctg
    10381 atcgagtcgc cccaccggcg ggttgcgata aagcgatgac tggcgcacca cccgcgtttc
    10441 gggcagcgct gccagtgcgc gcagcgcgga gcggatctga gccggcggat catcgaggtt
    10501 gctgcccagc gccacgaacg cagtgatgag tgatgagtga tgagtgatga gtggcaagac
    10561 tttagccttc caccgtcatc cttccgcctt catacttcgg cggccggcgc tgcgccgtgc
    10621 gccggaccgc cgtcggcacc gcgtgggcgc cgccggcggc gacggcggcg cctgcgcggc
    10681 tcttcgccgc tcaccagcat gtgggcgcgc tcggattccc cggcgcgctg gaagcgctcc
    10741 caccattcgc cgagcgcggg ttcgatctcg ccgctctcgc agcgcaggcg caggaaatcg
    10801 tagccggcgc ggaaacgcgg ctgctcgagc agccggaacg ggcgcttgcc ggcgcgctgc
    10861 aggaagcgcg gctgcatggc ccagatctcg cgcatgtcgg cgccgtagcg gtgcgggatc
    10921 gccagcttcc cggcctgggt ccgcatcacg tggtccatcg cccgatacag cgcggggacc
    10981 ggcggctcgc ccttctccgc gagcaccttg tactccgcct ggacctcggg ccacaacagc
    11041 gcggcgaaca cgaaacccgg cgagaccggc ttgccggcca gcacgcgctt gtccgtgttc
    11101 ctgagggcgg ccgtgataaa acgctccccc accgccctgc ccatggcggc gtccagtagc
    11161 ggaagcactc cgtggtgcaa cccttcccgg tgcagctgcg acatgccctc ggtcgcatgg
    11221 ccggacagca ggagcttcaa catctcgtcg agcagccgcg acggcggcac gtgcgcgagc
    11281 agtcccgcaa gcctgtgtat cgggcggcgg gtcggcgcgt cgatctcgag gccgcggctc
    11341 gccgccagcc gcacggcgcg cagcatccgt atcgggtcct cgcggtagcg ccgctccggg
    11401 tcgccgatca tgcgcagccg ccgtttcttc aggtcggcca cgccatcgtg atagtcccag
    11461 acttcccccg tggcggggtc gtagaacagc gcgttgatgg tgaagtcgcg ccgcgtggcg
    11521 tcctgcacct gggttccgaa cacgttgtcg cgcagtatcc ggccgtgctc gtccgccacg
    11581 tgatcgtcgg tttcatcgac cggattcatt ccgcgaaacg tggagacttc gaccgtttcc
    11641 gggccgcaca tcacatgcac caggcggaac cggcggccga tgatccgcga gcggcggaac
    11701 agctccctca cctcctcggg cgtggcatcg gttgcgacgt cgaaatcctt gggcttgcgg
    11761 ccgaggatga ggtcgcgcac cgcgccgccg acgacgaagg cggcgaagtc gtggcgctgg
    11821 agcgtctgcg tcgtgcgcag cgcgcacgag ctgatcgctc cgcggtctat gccgtgcgcg
    11881 ttgggcggga tgatcttcgg ctgggccggc ccgaacaacc ggccggagaa gacccggcct
    11941 agaaagcgcc taatcatcgg gggggatcac tatagtcatt gtcgatgggc cgccgcgcgc
    12001 tctcggtgat gcgtgatggg taatgggtaa tgggtaatgg gtaatgggca aaccgtcctc
    12061 ggtgcaagac ttccgcgcat tacccatcac tcattactca ttattaccgg tttaatcgag
    12121 gctgatgata cgccagcccg cgcgctcggc atggctgcgc agctccgggt ccggatccac
    12181 cgccacggga tgggtgacgc gcccgagcag cgccagatca ttgtgcgagt cgctgtagaa
    12241 ccacgactcg gtgaacgagg cgagcgaggc gccccgcccg gcgagccagg cgtcgagccg
    12301 cgcgaccttg ccctcgcgga agcaggggat gccggagacc gccccggtga attcgccgtc
    12361 ccgctgctcg ggctcggtgg caatcaggtc cgcgatcccg aactcgcgcg cgatcggcgc
    12421 ggtgacgaaa ctgttggtgg ccgtgatcac cacgcgaaga tcgccgcggt gccgctcgac
    12481 cagcgtgcgc gccttggcgc gcacgatcgg ccggatcttg agcgccatga actcgcgatg
    12541 ccacgcgtcg agttgcgccc gggggtggcg cgacagcggc ttcaactgga aatcgaggaa
    12601 agcgtggatg tccagcgtgc cggccttgta ttcgtcgaag aagcgctggt tgcgcgcctc
    12661 gtacacctcg cggtcgagca cgccctgctc gatcaggaac tgcgcccact cgaaatcgct
    12721 gtcgccggcg agcagcgtgt tatcgagatc gaacaggacc a
//
LOCUS       DBNGWZ010000322         4943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2555571, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000322 DBNGWZ010000000
VERSION     DBNGWZ010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2555571"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="AC16CX_09335"
     CDS             complement(<1..634)
                     /locus_tag="AC16CX_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012751.1"
                     /translation="MTILGYRIPALRETFRLPPSAFRLAIAALVLAPSAAFAQGYPAK
                     PIRLIVPFPPGGGTDVSARILAQALAEGPGWQLVVENRPGATGRIGTELAAKAPPDGY
                     TLLLGTAGPNAILPAAYPKLPYDAIKDFAPVTLVDSADYVLVVHPSLPVRSVKDMIAL
                     ARSKPGQITFASAGNLSVGHMAGELFKHYAKVDMTHIAYKGGGPAVIATVS"
     gene            complement(631..3012)
                     /locus_tag="AC16CX_09340"
     CDS             complement(631..3012)
                     /locus_tag="AC16CX_09340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012752.1"
                     /translation="MDQPLNAGRAGIGDSVRRKEDPRLIIGQGRFSDDIRLEGEVRAV
                     FVRSPHAHARIRAIDVRPALTVPGVIAVLTGKDLLADGLKPIPHKPLALHPAEPRLAN
                     RDGSPIFDGPHYPLAHDRARHVGEAVAVVIADTVDIAKDGAEQVEVDYEVLPAVTATL
                     AATEPDAPRLWDTASSNICLDAELGSQEATAAAFVRAAHVVKLDTWIQRVTGVPMEPR
                     AAVGEYDAATQRYTLHAGNGGAVRLKQDLALVLGVPAERVRVLIGDVGGNFGTRGMIY
                     PEFAVVAWAARRIGRPVKWTCERSEAFVSDYQGRDLAVTAELALDADGNFLAMRGSNV
                     SNVGSHPTNFGPLKKGVEIMSSIYRMPAAHFRARAVMSNTPPTRPYRSAGRPEVMFVM
                     ERMIDLAAREFGFDRVELRRRNLLTPSELPYPNPFGLVYDSGDYPEAMERALRLGDWA
                     GFPARRAEARARGKCRGIGIANYVDTATGVPRERADVTVRPDGHVDVVIGVVSQGQSH
                     ETTFAQLVTEWLGVPFERVRVIAGDTDIVKVGGGAHSGRGMRMGSVVIWNSVQEILRK
                     GTRIAARLLEADPSVIRFEKGGFRVQGAERSVGLFEVAGAASRLSDLPADLRGPLYAF
                     SDINFNDASFPYGCHVCEVEVDPELGTVEIARYSAVDDVGRAVNPMVVHGQIHGAIAQ
                     GVGQALLEHCVFDGAGQPLAGSFMDYSMPRADNFPFFATEISEVPCTTHPLGMRPAGE
                     GGITPALGVVMNAVVDALSEYGVKHIEMPATPERIWRAIREGKAKVESRKAEV"
     gene            complement(3014..3985)
                     /locus_tag="AC16CX_09345"
     CDS             complement(3014..3985)
                     /locus_tag="AC16CX_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012753.1"
                     /translation="MHRLMIAMVLAAAGAPAAAQTGTAPAYPSKVIRVVVPYAPGAGA
                     DLHARTLAQKMTENLGLAVIVENRSGANGIPAMELVAKSPPDGYTIVYALPAQYAVNP
                     ALYPKLPYDPVRDFDPIMLVVRTPLVLFAHPSVPASSVKELVQLAKARPGALTLASAG
                     NGSAGHLCLEMFRTLTGTNILHVPYKGAAPSMVDLLAGHAQLSFLAWSTAGGHVKTGK
                     LRALGVTQAKRATALPDLPAIAETLPGYDIANWYGVAGPKGMPRAVVTKLNAEIQRAL
                     NTPELRKGFEREAIEPIGGTPEAFGEYIKSEMVKWGRLVKASGVKID"
     gene            complement(3997..4884)
                     /locus_tag="AC16CX_09350"
     CDS             complement(3997..4884)
                     /locus_tag="AC16CX_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012754.1"
                     /translation="MPTTTSFVPVAPTISGPIADTRRPRVKFPAGACDCHAHVFGPQQ
                     QYPFAPGGGYVPPEASINDYVRMLRTIGCERAVIVHPSVYGTDNRCTVDALRSGKFPF
                     RGVVVIDDKTTDSQLEDMHQAGVRGVRINLKSKGAVLGIDTAPRLAERIKARGWHIQF
                     FLDTNTMPDIDQKMVKLPVDIVIDHFGHIETAHGMQAPGFQTLLRLTRHERCWFKLIG
                     PYRISRQVPKFADVIPFARALVEAAPDRCVWGTDWPHPNAKYMPNDGDLADMLAEWVP
                     DEKLRNRILADNPARLYDF"
BASE COUNT          758 a         1650 c         1716 g          819 t
ORIGIN      
        1 cgctcaccgt cgcgatgacg gcgggtccgc cacccttgta cgcgatgtgc gtcatgtcca
       61 ccttcgcgta gtgcttgaac agctcgcccg ccatgtggcc gacggagaga ttgcctgcgg
      121 aggcgaaagt gatctgaccg ggcttgctcc gtgccagggc gatcatgtcc ttaacggagc
      181 ggaccggcag cgacgggtgg acgaccagca cgtaatcggc cgagtccacc agggtgaccg
      241 gcgcgaagtc cttgatcgcg tcgtacggca gcttcgggta ggcggcaggc aggatcgcgt
      301 tcggcccggc ggtgccgagc agcagcgtgt agccgtcggg cggcgccttc gccgcgagct
      361 cggtgccgat gcggccggtc gcgcctggcc ggttttccac gacgagctgc cagcccgggc
      421 cttcggccaa tgcctgcgcg aggatgcgcg cggagacgtc cgtccctcca cccgggggaa
      481 acggcacgat cagccggatc ggcttggccg gatagccttg cgcgaacgcc gcagatggtg
      541 cgagcaccag cgcggcgatc gcaaggcgaa aggcggaagg cggaaggcga aaggtctccc
      601 gcagtgcggg gatgcgatag ccaagaatcg tcatacttcc gcctttcgac tttcgacttt
      661 cgccttgcct tctcgtatcg cgcgccagat gcgctccggc gtcgccggca tctcgatatg
      721 cttcacgccg tactcggaga gtgcgtcgac gacggcgttc atgaccacgc cgagcgccgg
      781 cgtgatgccg ccttcgcccg ctggccgcat gccgagcggg tgcgtggtgc aaggcacctc
      841 gctgatctcg gtcgcgaaga acgggaagtt gtcggcccgc ggcatggagt aatccatgaa
      901 cgagccggcg agcggctggc cggcgccgtc gaagacgcaa tgctcgagca gcgcctggcc
      961 cacgccctgc gcgatcgcgc cgtggatctg gccgtggacg accatcgggt tcaccgcgcg
     1021 gcccacgtcg tccaccgcgg aatagcgcgc gatctcgacc gtccccagct cgggatcgac
     1081 ctcgacctcg cagacgtggc agccgtaggg aaagctcgcg tcgttgaaat tgatgtccga
     1141 gaacgcgtag agcgggccgc gcaggtcggc gggtagatcg ctcaaacgcg aggcggcgcc
     1201 tgcgacctcg aacaggccga ccgagcgctc cgccccttgc acgcggaagc cgcccttctc
     1261 gaagcggatg acgctgggat cggcttccag caggcgcgcc gcgatgcgcg ttcccttgcg
     1321 caggatctcc tgcacggagt tccagatgac gacgctgccc atgcgcatgc cgcgaccgga
     1381 atgcgcaccg ccgccgactt tcacgatgtc ggtgtccccg gcgatgacgc gcacgcgctc
     1441 gaacggcacg ccgagccatt cggtcaccag ctgcgcgaaa gtcgtctcat gactctggcc
     1501 ctgggagacg accccgatca cgacgtcgac gtggccgtcg ggacgcacgg tgacgtcggc
     1561 gcgctcgcga gggacgccgg tggccgtgtc cacgtaattg gcgataccga tgccgcggca
     1621 tttgccgcgg gcccgcgctt cggcgcggcg cgcgggaaag cccgcccagt cgccgagcct
     1681 gagcgccctt tccatcgcct cggggtagtc gccgctgtcg tagaccaggc cgaacgggtt
     1741 gggataaggc agctcggacg gcgtgagcag gttgcggcgg cgcagctcga cgcggtcgaa
     1801 cccgaactcg cgagcggcga ggtcgatcat ccgttccatg acaaacatga cttccgggcg
     1861 gccggcgctg cggtacggtc gggtgggcgg ggtgttgctc atgacggcgc gggcgcggaa
     1921 atgcgccgcc ggcatgcggt agatgctcga catgatctcg acgcccttct tgagggggcc
     1981 gaaattggtc gggtgcgagc ccacgttgct cacgttcgag ccgcgcatgg cgaggaagtt
     2041 gccgtccgcg tcgagggcga gctccgcggt cacggcgagg tcgcggccct ggtagtcgct
     2101 gacgaacgcc tcgctgcgct cgcaggtcca cttcaccggc cgcccgatgc ggcgcgccgc
     2161 ccacgccacc acggcgaact cgggatagat catgccgcgc gtgccgaagt tgccgccgac
     2221 gtccccgatc agaacccgca cgcgctccgc cgggacgccg agcaccagcg caaggtcctg
     2281 cttcaggcgg accgcgccac cgttgccggc gtgaagcgta tagcgttgcg tcgccgcgtc
     2341 gtactcgccg accgcggcgc gcggctccat gggtacgccg gtcacgcgct ggatccaggt
     2401 atcgagcttg acgacgtgcg cggcgcggac gaacgccgcc gcggtcgcct cctgtgaccc
     2461 cagttccgcg tccaggcaga tgttcgagga tgccgtgtcc cacagccgcg gcgcgtcggg
     2521 ttcggtcgct gccagcgtcg cggtcaccgc gggcaggacc tcgtaatcca cttccacctg
     2581 ctcggcgccg tccttggcga tgtcgacggt atccgcgatg accaccgcga ccgcctcacc
     2641 gacgtgacgc gcccggtcgt gcgccaaggg atagtggggg ccgtcgaaga tcggggagcc
     2701 gtcgcggttg gcgaggcgcg gctcggcggg atggagtgcc agcggcttgt gggggatggg
     2761 cttcaggccg tcggcgagca ggtcctttcc cgtcaggacc gcgatgacgc ccggcaccgt
     2821 gagcgccggg cgcacgtcga tcgcgcggat gcgggcgtgg gcgtgcggag atcgcacgaa
     2881 caccgcgcga acctcgccct cgagccggat gtcgtcgctg aagcgcccct gccctattat
     2941 caggcgcggg tcttccttgc gccggaccga atctccgatg ccggcacgcc cggcgttcag
     3001 cggctggtcc atttcagtcg atcttcacgc cgctcgcttt caccagcctg ccccatttca
     3061 ccatctcgct ctttatatat tcgccgaacg cctccggggt gccgccgatc ggctcgatcg
     3121 cctcgcgctc gaaccccttg cgcagctcgg gggtgttcaa cgcgcgctgg atttcggcgt
     3181 tgagcttcgt gacgaccgct ctcggcatcc ccttcggccc cgccacgcca taccagttgg
     3241 cgatatcgta gcccggcagc gtctcggcga tcgccggcag gtcgggcagc gccgtggccc
     3301 gcttcgcctg tgtcacgccc agggcgcgca acttgccggt cttcacgtga ccgcccgccg
     3361 tgctccaggc gaggaaggaa agctgcgcgt gtcccgcgag caagtccacc atcgagggcg
     3421 ccgcgccctt gtacggcacg tgcaggatgt tggtgcccgt caatgtcctg aacatctcaa
     3481 ggcagagatg gccggccgag ccgttgcctg ccgacgccag cgtgagcgcg ccgggccggg
     3541 ctttggccag ttgaaccagc tcctttaccg agctggccgg cactgacgga tgcgcgaaca
     3601 gcaccagcgg ggttcgcacc accagcatga tcgggtcaaa atcccgcacc gggtcgtacg
     3661 gcagcttcgg gtagagggcg gggttgaccg catactgcgc gggcagcgcg taaacgatgg
     3721 tgtagccgtc gggcgggctt ttcgccacga gttccatcgc ggggatgccg ttggcgccgc
     3781 tgcggttctc gacgatcacc gccagcccga ggttttccgt catcttctgc gccagcgtgc
     3841 gcgcgtggag atcggcgccg gcgcccggtg catagggaac caccacgcgt atcaccttgg
     3901 atgggtacgc cggcgccgtg ccggtctgcg cggcggcggg cgcaccagcg gccgcgagca
     3961 ccatggcgat catcagtcga tgcatggaag ccgtgttcag aaatcgtata accgcgccgg
     4021 gttgtcggcg aggatgcggt tgcgcagttt ctcgtcggga acccactcgg ccagcatgtc
     4081 cgcgaggtcg ccgtcgttgg gcatgtactt cgcgttcggg tgcggccagt cggtgcccca
     4141 cacgcagcgg tcgggcgccg cctccaccag cgcgcgcgcg aacgggatca cgtccgcgaa
     4201 cttcggtacc tgcctggata tccggtaagg cccgatcagc ttgaaccagc agcgctcatg
     4261 gcgcgtgagc ctcaacagtg tctggaagcc cggcgcctgc atgccatgcg cggtttcgat
     4321 gtggccgaag tggtcgatga cgatatcgac gggcagcttc accatcttct ggtcgatatc
     4381 gggcatggtg ttggtgtcga gaaagaactg gatgtgccag ccgcgcgcct tgatgcgctc
     4441 ggccaggcgc ggcgcggtat cgatgcccag gacggcgccc ttggacttga gattgatccg
     4501 cacgccgcgt acgcccgcct ggtgcatgtc ctcgagctgg gaatcggtgg tcttgtcgtc
     4561 gatcaccacc acgccgcgga acgggaactt gcccgagcgc agggcatcca ccgtgcagcg
     4621 gttgtcggtg ccgtagacgc tggggtgcac gataaccgcg cgctcgcagc cgatagtgcg
     4681 cagcatgcgc acgtaatcat tgattgacgc ttccggcggc acgtagccgc cgccgggcgc
     4741 gaacggatac tgctgctgcg gcccgaacac atgcgcatgg caatcgcagg cgccggcggg
     4801 aaacttgacg cgcgggcggc gcgtgtccgc aatcgggccg gaaatggtcg gggcaacggg
     4861 aacgaacgag gtggtagtgg gcatgatttt cctattgatg gcgcggttat ccgatatgca
     4921 cgctggcgct ttccatggcg ttg
//
LOCUS       DBNGWZ010000323         3735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_38412, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000323 DBNGWZ010000000
VERSION     DBNGWZ010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_38412"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="AC16CX_09355"
     CDS             <1..314
                     /locus_tag="AC16CX_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012755.1"
                     /translation="NIGELETAMREKIPVVCVVFNDQGLGNERAFQNEHYGGRFYAVD
                     YRNPDFGALAKVFGAHGEHVTKPGELEGALRRAFDSGKPAVVDVMIDQKTLAPVVYRP
                     A"
     gene            complement(549..2912)
                     /locus_tag="AC16CX_09360"
     CDS             complement(549..2912)
                     /locus_tag="AC16CX_09360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012756.1"
                     /translation="MAQPARSARIGEPVRRKEDLRLVTGAGCFSDDVNLPGQAYAVML
                     RSPHAHARIRAIDSWKALAVPGVIAVLTGADLRSDGLKHIPYNPFTLHPADIRMSNTD
                     GSPPFVVPCYSMAVDRARYVGEIVAVVIAETTNIAKDAAEQVEVDYEVLPAVTRTVAA
                     AQPDAPRLWDEASSNVCLDAEVGARDATTAAFARAAHVAKIETWIQRVTGAPMEPRAA
                     VGEYDPATQRYTLRAGSGGAVRLKNDLAKILDVPAKSVRVLMDDVGGNFGTRGMIYPE
                     FALVPWAARRVGRPVKWTCERHEAFLSDFQGRDLAVTAELALDVEGNFLAMRGSNIGN
                     SGGHPGSFTPLQKGVEIMSSLYRMPAAHFRARAVVSNTAPTRPYRSSGRPEVMYVVER
                     LIDLAAREFGFDRVELRRRNLLKQGELPYTNPFGMVYDSGDYPAAMEKALELGDWAGF
                     PARRVEARRRGKCRGIGIANYVDTATGAPRERAEVTVHPDGQVDIVIGTISNGQGHET
                     SFAQLVNEWLGVPIDNVRLITGDTDIVKVGGGAHSGRSMRMGSVVIWNSARQIIAKGT
                     RIAARLLESDPSTIRFENGWFRVEGTERSASLAEVAAAAARLGDLPEDLRGPLAAFSD
                     ETFNEASFPYGCHVCEVEVDPELGTVEIVRYSAVDDVGRAVNPMIIHGQIHGGIVQGV
                     GQALMEQCYYDPGTGQLLTASFMDYSMPRADNFPFFTTEISEVPCTTHPLGMRPAGEG
                     GTTPTLGVFINAVVDALADYGVKHVEMPATPERIWRAIQDARMKAEG"
     gene            complement(2926..>3735)
                     /locus_tag="AC16CX_09365"
     CDS             complement(2926..>3735)
                     /locus_tag="AC16CX_09365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3012757.1"
                     /translation="MYDFDYHRPKSLDDAAAMLSKHEDFKLLAGGMSLIPALKMRLAR
                     YSGLVDLGALDRLKGIRREGDAIVIGAMTTHAEVAASDEVARAIPALAALAGGIGDPL
                     VRNRGTLGGSIANADPAADYPAGVLGLGATVVTNRRSIAADEFFLGLFETALQPGEII
                     TSVRFPVPQAAGYCKHPQPASRFALVGVFVARTAAGVRVAVTGAAPSVFRFKQAEAAL
                     SKRFDPAALDGIALGPDGLNSDIHASADYRAHCVAVVARRAIVKAREAGAR"
BASE COUNT          606 a         1259 c         1276 g          594 t
ORIGIN      
        1 gcaatatcgg cgagctcgag accgcgatgc gcgagaagat ccccgtggtc tgcgtcgtgt
       61 tcaacgacca ggggctcggc aacgagcggg cgttccagaa cgagcactac ggcgggcgct
      121 tctacgcagt ggattaccgg aacccggatt tcggggcgct ggcgaaggtg ttcggcgccc
      181 acggcgagca cgtcacgaag ccgggcgagc tcgagggcgc gctgcgacgg gcattcgaca
      241 gcggcaagcc ggcggtcgtg gacgtcatga tcgaccaaaa gacgctcgcg ccggtcgttt
      301 acaggccggc ctgagccgcg cagactgacg gctgaccacg ggcagtccga aggatgaagg
      361 atgaacgtgg aaggatgacc gtcgcgctct gcgcgacaaa agcacgtccc gacctttcgt
      421 tacctacggt cggaacgccg tgggacgaag cacgaagccc cggtgcagta gtcaataacc
      481 gtccaccggt cggacgccag tgaaaggtcg ggactcttga accttcacgc gtagcggggg
      541 gtcatccttc atccctccgc cttcatcctt gcatcttgga tggcgcgcca gatgcgttcc
      601 ggcgtcgcgg gcatctcgac gtgcttcacg ccgtagtcgg cgagcgcatc gacgacggcg
      661 ttgatgaaga cgccgagggt gggcgtggtg ccgccttcgc ccgccgggcg catgccgagc
      721 gggtgcgtgg tgcagggcac ctcgctgatc tcggtggtga agaacggaaa attgtccgcg
      781 cgcggcatcg agtagtccat gaacgacgcc gtcaagagct gcccggtgcc cggatcgtag
      841 tagcactgct ccatgagcgc ctgcccgacg ccctgcacga tgccgccgtg gatctggccg
      901 tgaatgatca tgggattcac cgcgcggccc acgtcgtcca ccgccgaata gcgcacgatc
      961 tcgaccgttc ccaactccgg atcgacctcg acctcgcaga cgtggcagcc gtaggggaag
     1021 cttgcttcat tgaaggtttc gtcggagaag gcggccagcg gcccgcgcag gtcctcgggc
     1081 aagtcgccca accgggcggc ggccgccgcg acctcggcga ggctcgccga gcgctccgtc
     1141 ccctccaccc ggaaccagcc gttctcgaag cggatggtgc tggggtcgct ctccagcagg
     1201 cgcgccgcga tgcgcgtgcc cttggcgatg atctggcgcg cggaattcca gatgacgacg
     1261 cttcccatgc gcatgctgcg gccggaatgc gcgccgccac cgaccttcac gatgtccgta
     1321 tccccggtga tcagccgcac attgtctatc ggcacgccca gccattcgtt cacgagctgg
     1381 gcgaagctcg tttcgtggcc ctggccgttc gagatcgtcc ctatgactat gtcaacctgt
     1441 ccatcggggt gcaccgtgac ctcggcccgc tcgcggggag cgccggttgc ggtgtcgacg
     1501 taattggcga tgccgatccc gcggcacttg ccgcggcggc gcgcctcgac gcggcgcgcc
     1561 ggaaagcccg cccagtcgcc gagctcgagc gccttttcca tggcagcggg ataatcgccg
     1621 ctgtcgtaga ccatgccgaa cggattggtg tagggcagtt cgccttgctt gagcaggttg
     1681 cggcgccgca gctcgacgcg atcgaagccg aactctcgtg cggccaggtc gatgagccgc
     1741 tccacgacgt acatgacttc cgggcggccg gaactgcggt acggccgcgt gggcgccgtg
     1801 ttgctcacga cggcgcgcgc gcggaaatgc gctgccggca tgcggtacag gctggacatg
     1861 atctcgacgc ccttctgaag cggcgtgaag ctgccggggt gcccgccgga attgccgatg
     1921 ttcgagccgc gcatggcgag gaagttgccc tcgacgtcga gagcgagctc cgccgtcacg
     1981 gcgaggtcgc ggccctggaa atcactgaga aaggcctcgt gccgctcaca ggtccacttc
     2041 accgggcgcc cgacacgccg cgccgcccac ggcaccaggg cgaactcggg gtagatcatc
     2101 ccgcgcgtgc cgaaattgcc gccgacgtcg tccatgagca cgcgaacgct cttcgccgga
     2161 acgtcgagga ttttcgcaag gtcgttcttc aaacggaccg cgcccccgct tccggcacgc
     2221 agcgtgtagc gttgcgtcgc cgggtcgtac tcaccgaccg cggcccgggg ctccatgggg
     2281 gcgcccgtga cccgctgtat ccatgtctcg atcttcgcga catgcgccgc gcgggcgaac
     2341 gccgccgtgg tcgcgtccct ggcccccact tccgcgtcga ggcagacatt ggatgacgcc
     2401 tcgtcccaca ggcgcggcgc gtcgggctgt gccgccgcga ccgtgcgggt gaccgcggga
     2461 aggacctcgt aatcgacctc gacctgctct gcggcgtcct tcgcgatatt cgtggtctcc
     2521 gcgatcacca cggctacgat ttcgccgacg tagcgcgccc tgtccaccgc catcgaataa
     2581 cacggcacga cgaaaggcgg cgaaccgtcc gtgttggaca tccgtatgtc cgcaggatgc
     2641 agggtgaaag ggttatacgg tatgtgcttg aggccgtcgg aacgcaggtc cgcgccggtg
     2701 aggacggcaa tgacgccggg cacggccagt gctttccagc tgtcgatggc gcggatgcgc
     2761 gcatgcgcat gtggcgacct cagcatcacg gcataggcct gccccggaag gttgacgtcg
     2821 tcgctgaagc agccggcgcc ggtaacgagg cgcagatcct ccttgcgccg gaccggctca
     2881 ccgatgcggg cactcctggc gggctgggcc actggcaact ccccgctagc gcgcgccggc
     2941 ttcgcgcgcc ttcactatgg cgcgcctcgc cacgactgcg acgcagtgcg cccggtaatc
     3001 ggcgctcgcg tgaatgtcag agttgaggcc gtcggggccg agcgcaatgc cgtcgagggc
     3061 ggcgggatcg aatcgcttcg agagggccgc ctcggcctgc ttgaaccgga acaccgaggg
     3121 tgccgcaccg gtgacggcga cgcgcacgcc agcggccgtc ctcgccacaa acacgccgac
     3181 cagcgcgaaa cgcgaagccg gctgaggatg cttgcagtag ccggccgcct gcgggacggg
     3241 gaaccgcacc gacgtgatga tctcgcccgg ctgcagcgcc gtctcgaaca gccccaggaa
     3301 aaactcgtcc gcggcgatcg agcgccggtt ggtcacgacg gtcgcgccca ggcccagcac
     3361 gcccgcggga taatcggcgg cgggatcggc gttcgcgatg gagcctccca gggtgccgcg
     3421 attgcgcacc aggggatcgc cgatgccgcc ggcgagggcc gccagcgccg gaatggcgcg
     3481 cgccacctcg tcggaggcgg ccacttcggc gtgcgtcgtc atcgcgccga tcacgatcgc
     3541 gtcgccttcg cgccggatgc ccttgagccg atcgagcgcg ccgaggtcca ccaggcccga
     3601 gtagcgggcg aggcgcatct tgagcgcggg tatcaggctc atgccgccgg cgagcagctt
     3661 gaagtcctca tgcttcgaca gcatcgcggc ggcgtcgtcg aggctcttag gcctgtggta
     3721 gtcgaagtcg tacat
//
LOCUS       DBNGWZ010000324         3934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1423629, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000324 DBNGWZ010000000
VERSION     DBNGWZ010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1423629"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="AC16CX_09370"
     CDS             <1..1203
                     /locus_tag="AC16CX_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3012758.1"
                     /translation="SNAQSRVLEHALFTAGVPYRVYGGLRFFERQEVKHALAYLRLVA
                     NPDDDSALTRIVNFPARGIGSRSLEQLADLAKEKSISLWAAALLKAEGRKQKDEGKEG
                     EADNSSLITHHSSLGKGIPAFVTLIEQMREATKGLPLPEVIEHVVEASGLKAHYQAER
                     EGADRLENLAELINAAATFVAERDVQPAGEAAGMDEPDELTAFLAHAALEAGEHQAEA
                     GTDALQLMTAHSAKGLEFHSVFVSGLEEGLFPHENSLTEADGIEEERRLMYVALTRAR
                     RRLYLSYAQSRMLHGQTRYGIASRFFHEIPENLMRRINPVSTARHAPSRKSAGLNAGY
                     SSPITHHSSLGPDSPWRVGQSVVHPTFGAGVIVSAEGRGPDARVQVNFRSGGLKWLML
                     EYAKLLPA"
     gene            complement(1376..2152)
                     /locus_tag="AC16CX_09375"
     CDS             complement(1376..2152)
                     /locus_tag="AC16CX_09375"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HIR3012759.1"
                     /translation="MEVRVVEAARGWQWIVDGFALFRKSPAMWIALTILLALLWVVSF
                     IIPVLGPLLFNLLSPVLFAGLMIGCRALENSGALEIHHLFAGFKQQAAPLVTIGGVYL
                     VGTIIVMGIVLLAAGGSTLPAVLSKSGTDIETLRAAVRSMAIALAIGAAAYLPLLMLV
                     WFAPLLVVFDGLAPLAAMKLSFTACLRNTLPFLVYGVVVLMLWIVLSLPAVLGAIGAL
                     LVIALLATSIPVLICSIYASYKDVFTAKDFARPRPGEILK"
     gene            complement(2451..3401)
                     /locus_tag="AC16CX_09380"
     CDS             complement(2451..3401)
                     /locus_tag="AC16CX_09380"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3012760.1"
                     /translation="MSVFTPVTREQLSAWLKHYSIGTLAALEGIPAGIENTNYFVTTS
                     QGRFVLTLFEKLKSHELPFYLNLQDHLAGQGVPCPKPVANNAGEFLGALNGKPAALVR
                     FLSGKDVAAPTAGQCAQAGAMLARIHVAGSSYAGRMDNPRGPKWWKSVMPEIVPFLGA
                     GDVTLLEDEVRFQSQNRSRDLPRGAIHADFFRDNVLFENGRISGVIDFYFACTDALLY
                     DVAITVNDWCVAEGGTLDGGRAAALLEAYGKVRSFTVVEREAWQLMLRAGALRFWVSR
                     LYDFYLPRPGELTHAKDPGHFRNILAAHIARGDSLPRLVP"
     gene            complement(3477..3821)
                     /locus_tag="AC16CX_09385"
     CDS             complement(3477..3821)
                     /locus_tag="AC16CX_09385"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR3012761.1"
                     /translation="MKIRLLPAALLLAVALPVAAQQSKPPALQPVPEPPPPPPGYELD
                     PALEPQVTILKRGTDTVEEYRIGGKLYMIKVTPARGAPYYLVDHRGDGTFVRQDNYDS
                     GIRPPMWVIFSF"
BASE COUNT          746 a         1258 c         1290 g          640 t
ORIGIN      
        1 tccaacgcgc aatcgcgggt gctggagcac gccctcttca ccgccggcgt gccgtaccgg
       61 gtgtacggag ggctgcgctt cttcgagcgc caggaagtga agcatgcgct cgcttatctg
      121 aggctggtcg cgaaccccga cgacgatagt gctctgacgc gcatcgtcaa ttttcccgca
      181 cgcggcatcg gcagccgcag cctcgagcaa ctcgccgacc tcgcgaagga gaagagcatc
      241 agcttgtggg cggcggcgct tctgaaggca gagggcagaa agcagaagga tgaagggaaa
      301 gaaggcgaag ccgacaactc atcactcatc actcatcact cgtcacttgg caaaggcatc
      361 ccggctttcg tcacactgat cgaacagatg cgggaagcga cgaaaggcct gcccttgccg
      421 gaggtcatcg agcacgtggt cgaggcgagc gggctgaaag cgcactacca ggccgagcgg
      481 gagggcgccg accgcctgga gaacctcgcc gagctcatca acgccgcggc gaccttcgtc
      541 gcggaacgcg acgtgcagcc ggcgggagag gcggcgggca tggacgagcc cgatgagttg
      601 accgcgttcc ttgcccacgc cgcgctggaa gcgggcgagc accaggcgga ggccggaacg
      661 gatgcgctgc agctcatgac cgcgcattcg gcgaaggggc tcgagttcca cagcgtgttc
      721 gtcagcgggc tggaggaagg cctgttcccg cacgagaaca gcctgacgga agccgacggc
      781 atcgaggagg agcggcggct gatgtacgtg gcgctcacgc gcgcgcgccg ccggctttat
      841 ctctcgtacg cgcagagccg catgctccac ggccagacgc gctacgggat cgcctcgcgc
      901 ttcttccatg agatacccga gaacctgatg cgccgcatca acccggtatc cacggcccgg
      961 catgcaccat ccagaaagag cgccgggcta aacgctggct actcatcacc catcacccat
     1021 cactcatcac tcgggcccga ttcgccttgg cgtgtcggcc agagcgtggt ccaccccacc
     1081 ttcggtgccg gcgtcatcgt cagcgcggag gggcgagggc cggacgcgcg cgtgcaggtc
     1141 aacttccgga gtggcggcct gaagtggctg atgctggaat acgcgaagct gctccccgca
     1201 tagccgcctg tcggagttgg ctgtccgaca ggcggctatg aagcgagcca tccggaaatt
     1261 tccccgtctc gacccggcgg ttttttcggg ggacaacgtc tttcacgcgc aggggccggg
     1321 attccctatg gattgcggaa tgatcccgga ctgcgcgctg cggaccgtcc cttctctatt
     1381 tcaggatttc gccgggccga ggtctggcga aatccttggc ggtgaagacg tccttgtagg
     1441 atgcgtagat cgagcagatc agcaccggga tcgaagtcgc cagcaacgcg atcaccagca
     1501 gcgctccgat ggcgcccagc accgccggca gcgacagcac aatccaaagc atcaggacca
     1561 ccacgccgta aaccaggaac ggcagggtat tcctgaggca tgcggtgaac gagagtttca
     1621 tcgcagccag cggcgccagt ccgtcgaaca ccaccagcag cggcgcgaac cagaccagca
     1681 tcagcagcgg aagataggcg gcggcgccaa tggcaagcgc gatcgccatg ctgcgcaccg
     1741 ccgcccgcag cgtctcgata tcggttcccg atttcgacag gacggcgggc agcgtcgagc
     1801 cgccggcggc cagcaacacg atgcccatca cgataatggt gccgacgagg taaacgccgc
     1861 cgatcgtcac cagcggcgcg gcctgctgct tgaagcccgc gaaaaggtga tggatttcca
     1921 gcgccccgct gttttcgagc gcgcggcaac cgatcatcag tcccgcgaag agaacgggcg
     1981 agagcaggtt gaacagcagc ggcccgagca ccgggatgat gaacgacacc acccacaaca
     2041 gcgcgagcag gatggtgagc gcgatccaca tcgccgggct cttgcggaac agggcgaatc
     2101 catccacgat ccactgccag cccctggctg cctcgaccac ccgtacttcc atgaatgttt
     2161 cccctgtccg cgtgtcccgc cgtcgttttc ggcacgttga agcgcgtcgc ctggcggcgt
     2221 cgccaccctg ttatcgcacg atgccgcttg cgtgtgctga tcccgagatc gattgttgtg
     2281 tcggcacgtc gagacgcgcc gtggcgtcgt caccaaccgc cacgcgatcc cgtttgcatg
     2341 cgtggatccc gagctcgatc gtcgtcccgg ctcgttgagg tgcgccgcgc cgcggagtca
     2401 cctcgaaccg ccatatgatc acgtttgcat gtgcgattga attatacggc tcaggggacc
     2461 agccgcggca ggctgtcccc gcgcgcaatg tgtgccgcca gtatgttccg gaaatgaccg
     2521 gggtccttgg cgtgggtgag ctcacccgga cgcggcagat agaagtcgta gaggcgcgaa
     2581 acccagaacc tgagcgcccc cgcgcgcagc atcagctgcc acgcctcgcg ctcgaccacg
     2641 gtgaaggagc ggacctttcc ataggcctcg agcagcgccg cggcgcgccc gccgtcgagc
     2701 gtgccgccct cggccacgca ccagtcattg acggtgatgg cgacgtcgta gagcagcgcg
     2761 tcggtgcacg cgaaatagaa atcgatcacc ccggagatgc gcccgttctc gaacagcacg
     2821 ttgtcgcgga agaagtcggc gtgaatcgcc ccccgcggga gatcccggga gcggttttgc
     2881 gactggaacc gcacctcgtc ctcgagcagc gtcacgtccc cggcgccgag gaacggaacg
     2941 atttcgggca tcacggactt ccaccacttc gggccgcgcg gattgtccat cctgccggcg
     3001 tacgagctgc cggcgacgtg gattctggca agcatcgcgc cggcctgggc gcactgtccg
     3061 gcagtgggcg cggcgacgtc cttcccggac aaaaatctca cgagcgcggc cggcttgccg
     3121 ttgagcgcgc cgaggaactc gccggcgttg ttggcgactg gtttggggca gggcacacct
     3181 tgccccgcca gatggtcctg caggttgagg tagaagggca gctcgtgcga cttgagcttc
     3241 tcgaacagcg tgaggacgaa ccggccctgc gacgtggtga cgaagtagtt ggtgttctcg
     3301 atcccggcgg gtatgccctc gagggcggcg agggtgccga tggagtagtg cttgagccag
     3361 gcgctgagct gttcgcgagt gacgggggtg aaaacggaca tgttctagct gtcagccatc
     3421 agcggtcagc cgccagctta aaccatgacg gcctcgtcac aaacacaagc tgaatgctag
     3481 aagctgaaga tcacccacat cggcggccgg atgccgctgt cgtagttgtc ctggcgcacg
     3541 aacgtgccgt cgcccctgtg atccaccagg taatagggcg cgccccgcgc cggcgtgacc
     3601 ttgatcatgt agagcttgcc gccgatgcgg tattcctcga cggtgtcggt gccgcgcttg
     3661 agaatggtca cctgcggctc cagcgccggg tcgagctcgt agcccggcgg cgggggcggc
     3721 ggctccggca ccggctgcaa cgcgggcggc ttggactgct gggccgcaac cggcagggcg
     3781 acggcaagca gcagggcggc cgggaggagg cggatcttca tgtcttgtta gacccgccgc
     3841 cattatcaat gtttcacgcc gtaattaaaa gcaggattag gaatccagga ttgagtaaaa
     3901 acattggggg taacggtttc gctcaatcct aaca
//
LOCUS       DBNGWZ010000325         5827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1045074, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000325 DBNGWZ010000000
VERSION     DBNGWZ010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1045074"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..707)
                     /locus_tag="AC16CX_09390"
     CDS             complement(135..707)
                     /locus_tag="AC16CX_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012762.1"
                     /translation="MLYMEEGIKPIRRVVTGNDARGRSRVLFDSAAPNVNASAFKKGT
                     GMTDIWVFQRCPVVVSGERDDGNLPFHFEPPSAGGHLRIVQSDPKPRDYNPAGDQYIV
                     APHPPKKTEGGTWERGGQNLYTTRMHKSETLDYGILLAGERILILDDGEYVLKPGDTV
                     VQIGSWHAWGNPHEGIQMAFVMMGATFDDE"
     gene            complement(791..2491)
                     /locus_tag="AC16CX_09395"
     CDS             complement(791..2491)
                     /locus_tag="AC16CX_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012763.1"
                     /translation="MTGSRLFAEMMRGYGVTHIFFVPAFMLKAFAEMEDMPVGRIMVH
                     GEKAAAYMADGYARASGRPGICMAQMIGASNLAAGLRDAYMGGVPVIAITGGPTPQSR
                     YKHAYQEVDDVTQFEPITKFNAQVDHVTRMPDLLRQAFRMATSGAPGPVHLRIQSHLG
                     QMTELEAELDPLVENQYSRAPAFRPEPEMRRVQDALAALAAAKRPMIVAGGGVIRSGA
                     EPEVVELAEKLSIPVATSLNAKAAVLDAHPLNVGVPGAYSRDCANRALAEADLVFFIG
                     SHTGGQVTNNWMFPPAGTRVIQLDIDPGELGRNYPNAVSILGDAKTSLRRMIEAAPRE
                     ASAMTADWTKRVQRFVADWRAANAEMRDADIAPIRPERICKEISDVLPPDGVVVSDTG
                     HAGIWTARFIEFRHRGQGYFRTAGSLGWGFPAALGVKCAQPNRTVVCFTGDGGFYYHI
                     AELETARRHNISAVIVVNNNSALNQEIKLNDAAYGGKQRGRAEEMWRFPDVNFARVAE
                     SFGCAGIRVEDPRDLSGALKQAIAMNKPVVVDAVTDTCAIAPHPWTPSGRDFHTYQKG
                     "
     gene            complement(2601..4301)
                     /locus_tag="AC16CX_09400"
     CDS             complement(2601..4301)
                     /locus_tag="AC16CX_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3012764.1"
                     /translation="MPRMTGSRLFAEMMKGYGVTHVFFVPTILLEALAEMDTLGIRKI
                     LTHGEKAAAYMADGYARASGRPGVCLAQQIGASNLAAGLRDGYMACSPIIAVTGGPAT
                     PGRYRHAYQEVEDFTQFDAVTKLNIQLDDLARFPDLLRHLFRTATSGAPGPVHLRLRG
                     SHGQGVEGEADLQPLTEAQFARVPAFRPAPEPEQVRDAVAMLSKAQKPIIIAGGGVIA
                     SQARAELVGLAEKLQLPVATSLSAKDAILDTHPLSVGVVGIYSRACANRAVSESDLVF
                     FIGSHAGGQVTANWRIPAAGTPVIQLDIDAQQLGRNYPNAVSLLGDAKVALRQMADAA
                     QRKSPESAAAWTRRVRELVAEWRKEADRKRESGMVPLRPERLCKEVTEALPADGVVVS
                     DTGHAGMWTGQMVYLTQPGQRYIRCEGSLGWGLPGALGVKCALPDRPVACFTGDGGVY
                     YHLPELETAARHGINLVVVVNNNNSLNQEIPLVEAAYREKRNERSGEIWRFQKTADLA
                     KVAEALGCAGFRVEKPGELKELLPRAFGMNKPVVIDCISDEQALAPTAWVPGRTGGGH
                     "
     gene            complement(4414..5379)
                     /locus_tag="AC16CX_09405"
     CDS             complement(4414..5379)
                     /locus_tag="AC16CX_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012765.1"
                     /translation="MIISLRPLVGALLLTLVAGVTFAQAYPTRPIRIIALSSPGSGPD
                     IVGRLIGQKLTEAWGQQVIVDTRPGATGIIGAEIASKAAPDGQTLVIVTSQAVIVSVM
                     YEKLPYNLIRDFAPISLMASTPFILAVHPAVPAHSIKELIALAKSRPGQLHYGSGGSG
                     SPPHLSAEMFKSMTGIDIVHVPYKGITPALIDTAGGQVQMLISVIPAILATVRAGKVR
                     ALGVTSAQRTPLVPDLPTIAETVPGYEFIGWYSLVAPAKTPAGILSRLNAEVVRALKT
                     PEFRDRFSALGAEPIGSTQRALAEYLPVQMEKMRKAVKESGARPD"
     gene            complement(5498..>5827)
                     /locus_tag="AC16CX_09410"
     CDS             complement(5498..>5827)
                     /locus_tag="AC16CX_09410"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3012766.1"
                     /translation="LFAYPVKPDGGLGPCKVLIAFAPAERGIEGMCLDSEGNILACAG
                     WKRGGMGPAILVISPAGTVLETHSTPADMPMRCAFGEAGLASLYLTAGDGGLYRVTNT
                     GRRGLKR"
BASE COUNT          956 a         1983 c         1814 g         1074 t
ORIGIN      
        1 taaagcctgt acgcgccggg tcaatgcgca cgtccggtga cgggccgtcc gcgatcgccc
       61 gcgacctgtc gttctcatcc atcacgacga tcctgcgcac cggttttagt tcttcattca
      121 tcattcatca ctcatcattc atcatcgaac gtcgctccca tcatgacgaa cgccatctgg
      181 atgccctcgt ggggattgcc ccacgcgtgc cagctgccga tttgcaccac ggtgtcgccc
      241 ggcttcaaga cgtactcgcc gtcgtcaaga attaggatgc gttcgcccgc gagcaggata
      301 ccgtagtcga gcgtttccga tttgtgcatg cgcgtggtat agaggttctg gccgccgcgc
      361 tcccacgtgc ccccttccgt cttcttcggt gggtggggcg caacgatgta ttggtcgccg
      421 gccgggttgt agtccctggg cttggggtcc gactggacga tgcgcaggtg tccgccggcg
      481 gagggcggtt cgaagtggaa cggcaggttg ccgtcgtcgc gttcaccgga aaccactacc
      541 gggcaccttt gaaaaaccca gatgtcggtc atgcccgtgc ctttcttgaa ggcgctggcg
      601 ttgacgttcg gcgccgcgct gtcgaacaac acccgcgacc ttccccgggc gtcgtttccg
      661 gtcaccaccc gccggattgg tttgatgccc tcttccatat acaacacctc gttcctgtat
      721 acagccctca gcggtcagct atccgcttga aacgcagcag tgtttccgct gatcgctgaa
      781 agctcgcagc tcatcccttc tgataggtgt gaaagtccct gccgctcggt gtccacggat
      841 gcggcgcgat cgcgcatgtg tcggtgacgg cgtccacgac cacgggtttg ttcatcgcga
      901 tggcttgctt cagcgcgccg ctcagatccc ttggatcctc gacgcggatg ccggcgcacc
      961 cgaatgattc ggcgactctg gcgaaattga cgtcgggaaa ccgccacatc tcctcggcgc
     1021 ggccgcgctg tttgccgccg tacgccgcat cgttcagctt gatctcctgg ttgagagcac
     1081 tgttgttgtt gacgacgatc accgcgctga tgttgtggcg ccgcgcggtc tcgagctctg
     1141 caatatggta gtagaacccg ccatcgccgg taaaacacac gacggtgcgg ttcggctgcg
     1201 cgcacttcac cccgagcgcc gcggggaatc cccatcccaa actgccggcc gtgcgaaagt
     1261 agccctgccc ccggtgccgg aactcgatga agcgcgcggt ccagatgccg gcatggccgg
     1321 tatccgacac cacgacgcca tccggcggca gcacgtccga gatttccttg cagatgcgct
     1381 caggacggat cggcgcgatg tcggcgtcgc gcatttccgc gttcgcggcg cgccagtcgg
     1441 cgacgaatcg ctgcacgcgc ttcgtccaat ccgccgtcat cgcggaagcc tcgcgcggcg
     1501 cggcttcgat catccgccgc aggctcgtct tggcgtcccc cagaatcgaa acggcattcg
     1561 gatagtttct gccgagctct ccgggatcga tatcgagctg gatgactctt gtgcccgcgg
     1621 gcgggaacat ccagttgttg gtaacctgcc cacccgtatg gctgccgatg aagaacacga
     1681 gatcggcttc cgccagggcg cggttggcgc aatctcgcga ataagcgccc ggcacgccga
     1741 cgttgagggg atgcgcatcg agcaccgcgg ccttggcgtt gagagaagtc gccacgggta
     1801 tcgagagctt ctcggccagc tccacgacct cgggctccgc cccggatcgg atcaccccgc
     1861 cccccgcgac gatcatcggt cgcttcgccg cggcgagcgc tgcgagcgcg tcttggacgc
     1921 gccgcatctc cggctccgga cggaacgccg gagcacgcga atactggttc tcgacgagcg
     1981 ggtcaagctc ggcttccagc tcggtcatct ggcccagatg gctctggatg cgcaggtgca
     2041 cgggacccgg cgcgccggag gtcgccatgc ggaacgcctg gcgcagcagg tcgggcatgc
     2101 gcgtgacgtg gtcgacctgc gcgttgaact tcgtgatcgg ttcgaactgc gtgacgtcat
     2161 cgacttcctg gtaggcatgc ttgtatcgcg attgcggcgt gggaccgccg gtgatggcga
     2221 tgaccggtac gcctcccatg tacgcatcgc gtaacccggc ggcgagattc gacgcgccga
     2281 tcatctgcgc catgcagatg cccggcctgc cgctcgccct ggcatagccg tccgccatgt
     2341 aggctgcggc cttctcgccg tgaaccatga tccggcctac aggcatgtcc tccatctcgg
     2401 cgaaggcctt cagcatgaac gccggcacga aaaagatgtg agtgacgccg tagccccgca
     2461 tcatttccgc gaacagccgc gaacccgtca ttttcggcac cagacctcca agaaccaacc
     2521 agatattccc gatccgctcc ggatattcaa cgcaacatcc tcgctttctt cgcgctggga
     2581 ggtgcgcggg gccgttcttc tcaatgccca ccaccggttc tgcccggcac ccatgcggtc
     2641 ggggccagcg cctgctcgtc gcttatgcaa tcgatcacca ccggcttatt catgccgaac
     2701 gcgcgcggca gcaactcctt cagctcgccc ggcttttcca cgcggaaccc ggcgcacccc
     2761 agcgcctccg ccaccttggc caggtccgcg gttttctgga atcgccagat ctcgccggag
     2821 cgctcgtttc gcttttcgcg gtacgccgcc tcgaccagtg gaatctcctg gttgagagaa
     2881 ttgttgttgt tgaccacgac cacgaggttg atgccgtggc gcgccgcggt ctcgagctcc
     2941 ggcaggtgat agtacacgcc gccgtcgccg gtgaagcacg cgaccggccg gtcgggcagc
     3001 gcgcacttca cgcccagcgc gcccggcaag ccccatccca gcgagccctc gcagcggatg
     3061 tagcgctggc ccggctgggt caggtaaacc atctggcccg tccacatgcc ggcatgcccg
     3121 gtgtcggaaa ccaccacgcc gtcggccggc agcgcttccg tgacctcctt gcagaggcgt
     3181 tccggccgca gcggcaccat gcccgactcg cgcttcctgt ccgcctcctt gcgccattcg
     3241 gcgacaagct cgcgcacgcg cctggtccac gccgccgcgc tttcgggcga cttgcgctgc
     3301 gcggcgtccg ccatctgccg cagcgccacc ttggcatccc ccagcagcga caccgcgttc
     3361 gggtaattcc tgcccagttg ctgggcatcg atgtcgagct ggatcaccgg cgttcccgcg
     3421 gcggggattc tccagttcgc cgtcacctgc ccgccggcgt ggctgccgat gaaaaaaacg
     3481 agatcggact ccgacaccgc gcggttggcg cacgcgcgcg agtatatgcc gacgacgccg
     3541 accgacagcg ggtgggtgtc caggatggcg tccttggcgg agagcgaggt ggcgacgggc
     3601 aactggagct tctcggcgag ccccaccagt tcggcccgcg cctgggaagc gatcacgccg
     3661 ccgccggcga tgatgatggg cttctgcgcc ttggaaagca tcgccacggc atcgcgtact
     3721 tgctccggct caggggcggg acggaacgcc ggcacgcgcg cgaattgcgc ctcagtcagc
     3781 ggctgcaaat cggcttcgcc ctcgacgccc tggccgtgcg agccgcgcag ccgcagatgg
     3841 accggccccg gggcgcccga cgtcgctgtg cgaaacaggt ggcgcaacag gtccggaaag
     3901 cgcgcaaggt cgtcaagctg gatgttgagc ttcgtcaccg cgtcgaactg tgtgaaatcc
     3961 tcgacttcct ggtaggcgtg ccggtaacgt cccggcgtgg cgggcccacc ggtgacggcg
     4021 atgatcggcg agcaggccat gtagccgtcg cgcaggccgg cggcgagatt ggacgcgccg
     4081 atctgctggg cgaggcacac gccgggccgg ccgctcgcgc gcgcgtagcc gtccgccatg
     4141 tacgccgccg ctttctcgcc gtgcgtgagg attttgcgta tgcccagggt gtccatctcc
     4201 gcaagagcct ccagcaggat cgtgggcacg aagaacacgt gcgtgacccc gtaacctttc
     4261 atcatctccg cgaacagccg cgatccggtc atcctgggca tggttcctcc gtaatctggc
     4321 gatcagcttt cagctgtcag cactcagccg ttcgcgggca actgaacccg ttccattctt
     4381 ggctgaaagc tgagagctga cggctgacag cttctagtcc ggccgcgcgc cggactcctt
     4441 caccgccttg cgcatttttt ccatctgcac cggcaggtat tccgccagcg ccctctgcgt
     4501 cgagccgatc ggctcggcgc ccagcgcgga gaaccgatcg cggaattccg gggtcttcag
     4561 cgcacgcacg acctcggcat tgagccggga caatatgccc gcgggcgtct tcgccggcgc
     4621 gacaaggctg taccagccga tgaactcgta gcccgggacc gtttccgcga tcgtcggaag
     4681 gtccggcacg agcggcgtgc gttgcgcgct ggtgacgccg agcgccctca ccttgcccgc
     4741 cctgaccgtt gccaatatcg ccgggatcac ggaaatcaac atctgaacct gcccgcccgc
     4801 cgtgtcgatc agcgccggcg tgattccctt gtacggaaca tgcacgatgt cgatgcccgt
     4861 catgctcttg aacatctcgg cggacagatg cgggggcgaa cccgagccgc cggagccgta
     4921 atgaagctgc cccggcctcg acttcgcgag cgcgatcagt tctttgatcg agtgggccgg
     4981 aaccgcggga tggacggcga gtatgaacgg agtcgaggcc atcaggctga tgggggcgaa
     5041 atcccggatc aggttatagg gcagcttctc gtacatgacg gaaacgatga cggcctggga
     5101 agtcacgatc accagcgtct ggccgtccgg cgccgctttc gaagcgattt ccgcgccgat
     5161 gatgccggtc gcgcccgggc gcgtgtcgac aatcacctgc tgtccccagg cttcggtcag
     5221 cttctgcccg atcagccgcc cgacgatgtc gggacccgag ccgggcgagc tcaacgcgat
     5281 gatacggatc ggccgggtcg ggtatgcctg cgcgaacgtc actccggcaa cgagtgtgag
     5341 cagcaacgca ccgaccagtg gtcttagaga aataatcatg ctgcgttccg ttcgcgcgag
     5401 gcatgagtgt cgaggattga gtgtcgagtg cagtaagcgg cgaaccctga gccaaagctt
     5461 cttaccctcg acacccaaca ctctcactcc tcatgcgtca gcgcttgagc cctcggcggc
     5521 cggtattcgt cacgcgatac agcccgccgt cgccggccgt caggtacagg ctcgcaagac
     5581 ctgcctcgcc gaacgcgcag cgcatcggca tatccgccgg cgtggaatgg gtttccagca
     5641 cggtaccagc cggcgaaatc accaggatcg ccggtcccat gccgcctctt ttccagccgg
     5701 cgcaagcgag gatattgccc tcgctgtcga ggcacatgcc ctcgataccg cgctccgcgg
     5761 gcgcgaacgc gatcagcact ttgcacggtc cgaggccgcc gtccggtttc accggatagg
     5821 cgaagag
//
LOCUS       DBNGWZ010000326         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1423991, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000326 DBNGWZ010000000
VERSION     DBNGWZ010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1423991"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1805)
                     /locus_tag="AC16CX_09415"
     CDS             complement(<1..>1805)
                     /locus_tag="AC16CX_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3012767.1"
                     /translation="GEVLQPLDEERARTGLADAFRNGFRAAAIVLMHGYRYPAHEQRL
                     AALAREAGFSQVSVSHKVSPLMKLVARGDTTVADAYLSPLLRRYVGRVARDLPGARLM
                     FMQSNGGLTSAQLFQGKDSVLSGPAGGVVGVVRTALAAGFERIIGFDMGGTSTDVSHF
                     HGYFERTFDSNVAGVRLRAPMMSIHTVAAGGGSILHFDGARLRVGPDSAGAQPGPACY
                     RKGGPLTVTDANVLLGKIQPEFFPHTFGAAADAPIDSEVVREKFTALSEEIHRQTGKR
                     STPQEVAEGYVRIAVDNMANAIKKISIERGHDVTGYTLACFGGAAGQHACLVADALAI
                     PRVFIHPYAGVLSAYGMGLADLSALREQAIEARLDADLMPRLGETLAKLADTAREELG
                     GQGVPREGIHVARRVHLRYEGTDTAVIVGFGTPEEMTWQFESGYRGRFSFLMPGRALV
                     VEAVSVEAAGGGTPVRERFGELPARRGAAQPARRVRTHSGGAAHETPVYRRDALRPGD
                     VIPGPAIIAEANATTIVEPEWQAQVTTLNHLVLTRSRPRPQREAVGTHADPVMLEIFN
                     NLFMAIAEQMGVALAQTAHSVNIKERLDFSCALFD"
BASE COUNT          289 a          617 c          607 g          292 t
ORIGIN      
        1 catcgaacag ggcgcacgag aagtcgagcc gctccttgat gttgaccgag tgggcggtct
       61 gggcaagcgc cacccccatc tgctcggcga tcgccatgaa gaggttgttg aagatctcca
      121 gcatgacggg gtccgcatgg gtgccgaccg cctcacgctg gggtcttgga cggcttctag
      181 tcaacactag atggttgagt gtggtcacct gggcctgcca ctccggctcg acgatcgtcg
      241 tggcgttcgc ttccgcgatg atcgcgggtc ctggaatcac gtcgccgggt cgcagcgcgt
      301 cgcggcggta gaccggcgtt tcgtgcgccg ctccgccgct gtgcgtcctc acgcggcgag
      361 ccggctgcgc cgcaccccgg cgggcaggca gctcgccgaa ccgctcacgc accggcgtgc
      421 cgccgccggc cgcttcgacc gaaaccgcct cgacgaccag tgcgcgccct ggcatgagaa
      481 agctgaagcg gccgcggtag cccgactcga actgccaggt catttcctcc ggagtgccga
      541 agcccactat gaccgcggtg tcggtgcctt cgtagcgcag gtggacgcgt ctggcaacgt
      601 ggatgccttc ccggggcacc ccctgaccgc cgagttcttc gcgcgcagtg tccgccagtt
      661 tcgcgagcgt ttccccgagc cgcggcatca ggtccgcatc gagccgcgcc tcgatcgcct
      721 gctcgcgcaa agcgctcaaa tcggcaagac ccatgccgta ggcggaaagc actcccgcat
      781 agggatggat gaagacgcgc gggatcgcca gtgcgtcagc caccaggcag gcgtgctggc
      841 cggcggcccc gccgaagcag gcgagggtgt agccggttac gtcgtgcccg cgctcgatcg
      901 agatcttctt gatggcgttg gccatgttgt ccaccgcaat tcgcacgtaa ccctcggcga
      961 cctcctgtgg cgtggaccgc ttcccggttt gcctgtggat ttcttcggac agtgcggtaa
     1021 atttttcccg cacgacttcg gagtcgatcg gcgcgtcagc cgcggcgccg aacgtgtgcg
     1081 ggaaaaattc gggctggatc ttgccgagca ggacgttggc gtcggtgacc gtcagcggcc
     1141 cgcccttgcg atagcaggcc gggcccggtt gcgccccggc tgaatccggg cccacccgca
     1201 accgcgcgcc gtcgaaatgc aggatcgatc ccccgcccgc ggcgacggtg tgaatgctca
     1261 tcatcggcgc gcgcagccgc acgcccgcga cgttcgagtc gaaggtgcgt tcgaaataac
     1321 cgtgaaaatg cgacacgtcg gtggaggtgc cgcccatatc gaagccgatg atcctctcga
     1381 agccggcggc gagcgccgtg cgcaccacgc ccaccacgcc cccggcgggc ccggagagca
     1441 cgctgtcctt gccctggaac aactgcgccg acgtgagacc gccgttcgat tgcatgaaca
     1501 tgaggcgcgc gcccggcaga tcccgcgcga cgcgcccgac gtaacggcgc aggagcggag
     1561 agagataagc gtcggccacg gtcgtatcgc cgcgggcgac cagtttcatc agggggctca
     1621 ccttgtgcga gaccgagacc tggctgaagc ccgcttctcg cgcgagcgcg gcaagacgct
     1681 gctcgtgcgc ggggtagcgg tagccgtgca tcagcacgat ggcggccgcg cgaaagccgt
     1741 tccggaaggc gtcggcaaga ccggttcgcg cccgctcctc gtcgagcggt tgaagcacct
     1801 ccccg
//
LOCUS       DBNGWZ010000327         5143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_666026, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000327 DBNGWZ010000000
VERSION     DBNGWZ010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_666026"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="AC16CX_09420"
     CDS             <1..155
                     /locus_tag="AC16CX_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3012768.1"
                     /translation="FHFVVTGKGLKREQVERAIHLSAEKYCSASIMLGKTAEITHDFD
                     IREPQN"
     gene            complement(449..1084)
                     /gene="coq7"
                     /locus_tag="AC16CX_09425"
     CDS             complement(449..1084)
                     /gene="coq7"
                     /locus_tag="AC16CX_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HIR3012769.1"
                     /translation="MPALPSLDELIVAFDRGLRTVFAPAQSLRATPGENLPEAGLTEE
                     QRRLSASLMRVNHTGEICAQALYQGQALTARDTRARAALEQAAREETEHLAWTERRIE
                     ELGGRKSLLNPAWYAGSFALGALAGLVGDRWNLGFLAETERQVVAHLEGHLQRLPAED
                     SRSRAIVETMKEDEARHATSAIKHGAAELPPPAKAAMRLSSKVMTETAFWI"
     gene            complement(1117..1914)
                     /gene="trpC"
                     /locus_tag="AC16CX_09430"
     CDS             complement(1117..1914)
                     /gene="trpC"
                     /locus_tag="AC16CX_09430"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR3012770.1"
                     /translation="MADVLQRILAVKAKEVARAKGAKPPGALRDEARRVAPARDFIGA
                     LRSKITAGKPAVIAEIKKASPSRGVLRENFDPGAIAASFERHGAACLSVLTDERFFQG
                     GLEHLKQARAACKLPVLRKDFTIDPYQVYEARAAGADCILLIVAALDGPRMRELEAAA
                     SEVGMAVLVEAHDGGELEQALRLNTPLIGINNRNLRTFETRLETTLDLVAQVPPERMV
                     VTESGILSPDDVQTLRDGRVSCFLVGEAFMRAADPGAELARLFGAAP"
     gene            complement(1907..2935)
                     /gene="trpD"
                     /locus_tag="AC16CX_09435"
     CDS             complement(1907..2935)
                     /gene="trpD"
                     /locus_tag="AC16CX_09435"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3012771.1"
                     /translation="MTPQEALTRVIEHREIFREEMLSLMRAIMSGEVSPTLIAAIIIG
                     LRVKKETIGEIAAAAEVMREFATKVPIRDSDSLIDIVGTGGDGAHTFNISTAATFVCA
                     AAGGRVAKHGNRSVSSKSGSADVLEALGANINLKPEQIGRCIEEVGVGFMFAPNHHGA
                     MKHAAPVRRELGVKTIFNILGPLTNPAGARLELMGVFHPDLVGIQARVLQRLGSRHVM
                     VVHGLEGMDEISIAGPTMVSELKDGKISEYNIAPQDFGLKTHGGSAVRVESVEQSKAL
                     VLAALNNEPGPARDIVALNAGAGIYVAGLAPTLAGGVQKALAVMASTAPRKKLDAFVG
                     FTQTQANG"
     gene            complement(2932..3504)
                     /locus_tag="AC16CX_09440"
     CDS             complement(2932..3504)
                     /locus_tag="AC16CX_09440"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3012772.1"
                     /translation="MLLMIDNYDSFTYNLVQYLGELGQEVKVCRNDEITLEGIGKLRP
                     AHVVISPGPCTPNEAGISVPLIKEFAGRIPILGVCLGHQAIGQAFGGKIVHAKQLMHG
                     KTSAIRHDGRGVFRGLPDAFTATRYHSLVIERAGLPGCLEVSAWTDDGEIMGVRHKEL
                     AVEGVQFHPESILTEHGHHLLRNFLEGGKP"
     gene            complement(3614..5089)
                     /gene="trpE"
                     /locus_tag="AC16CX_09445"
     CDS             complement(3614..5089)
                     /gene="trpE"
                     /locus_tag="AC16CX_09445"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR3012773.1"
                     /translation="MTEAEFNRLAAAGYNRAPIVLETFADLDTPLSIYLKLANRRHSY
                     LLESVVGGERFGRYSFIGLPAQTRIEVRGETCAEVRDGQLVGEKRVSDPLAYVNDYLA
                     RFKVAQDASLPRFCGGLVGYFGYDTVRYIERRLASSRKQDEIGAPDILLLVSEEIAIV
                     DNLSGKLTLVVYAEPGKRDAFQSARARLKQLLAKLREPVAIPVDARGPSEPAVSGFGE
                     SAYHKAVERAKRYIFEGDIMQVVPSQRLSKPFRASPLALYRALRTINPSPYMYYFDFD
                     GFHVVGASPEILARLEGDLVTVRPIAGTRRRGATPAEDAALEKELLADEKERAEHIML
                     VDLGRNDAGRVAQTGTVRVTEQMVVERYSHVMHIVSNVEGRLRPGLNAIDVLRATFPA
                     GTVSGAPKVRAMEIIDELETVKRGIYAGAVGYLGFHGNMDVAIALRTAIVKNGTLYVQ
                     AGGGIVADSQPAAEWQETQSKARALLRAAEMAESGLDTRMD"
BASE COUNT          854 a         1716 c         1628 g          945 t
ORIGIN      
        1 acttccactt cgtcgtgacc ggaaagggcc tgaagcgcga gcaggtggaa cgcgccattc
       61 acctgtcggc ggagaagtac tgctcggcct cgatcatgct cggcaagacc gccgagatca
      121 cgcacgattt cgacatccga gaaccccaga attgaaccgc caagacgcca agagaagcag
      181 atacaaaaat agcacagaat atcaaaccct atccaacctc cgagactagg acagaaccaa
      241 gaacgagaac ggaaataaac tcaaaatgag cggttttcga aattaatata atttctgtct
      301 tcgctactct tcgcatctcc gcggttcaat tattgttctt gtttttctcg gcgtgcttgg
      361 cgtcttggcg gttcaatttt gttcttgtct tttctagtcg tctttggcgg ttcgttccgg
      421 atcttgatct tcccggcggt tctggcggtt aaatccagaa cgcggtttcg gtcatcacct
      481 tcgacgacag ccgcatcgcg gcctttgcgg gtgggggcag ctccgccgcg ccgtgcttga
      541 tcgcgctggt ggcatggcgc gcctcgtcct ccttcatggt ctcgacgatc gcccgacttc
      601 tgctgtcctc cgcgggcagc cgctgcaagt gcccttcgag gtgcgcgacg acctggcgct
      661 cggtctcggc gaggaaaccc aggttccagc ggtcgccgac gagccctgcg agcgcgccga
      721 gcgcgaacga gccggcgtac caggcagggt tcaacaggct cttgcgaccg ccgagttcct
      781 cgatccgccg ctcggtccag gcgaggtgct ccgtctcctc ccgcgccgcc tgttccagcg
      841 ctgccctggc ccgcgtatcg cgggcggtga gcgcctgccc ctggtagagc gcctgcgcgc
      901 agatctcgcc ggtgtggttg acccgcatca gcgacgcgga gagccggcgt tgctcctccg
      961 tcaggccggc ctcaggcaga ttttcgcccg gcgttgcgcg cagtgactgc gccggcgcga
     1021 acaccgtgcg caagcccctg tcgaaggcga cgatcagttc atcgagcgaa ggaagcgcgg
     1081 gcatgaatcg aggctctcgg tttcagtata gcggcctcac ggggcggcgc caaacaggcg
     1141 ggcaagctcc gcgccggggt ccgccgcgcg catgaatgcc tcgccgacca gaaagcagct
     1201 caccctgccg tcgcgcaggg tctgcacgtc gtccggcgac aggatcccgc tctccgtcac
     1261 taccatgcgt tccggcggga cctgcgcgac caggtcgagc gtggtctcaa gccgcgtctc
     1321 gaacgtgcgc aagttgcgat tgttgatgcc gatgagggga gtgttgagcc tgagcgcctg
     1381 ctccagctcc cccccatcgt gtgcctcgac cagcaccgcc ataccgacct cgcttgccgc
     1441 cgcctccagt tcccgcatgc gcgggccgtc aagcgccgcc acgatgagca ggatgcaatc
     1501 cgcgcccgcc gcgcgcgcct cgtatacctg gtagggatcg atggtgaagt ccttgcgcag
     1561 gaccggcagc ttgcacgccg cgcgcgcttg cttcaggtgc tcgagcccgc cctggaaaaa
     1621 ccgctcgtcg gtcagcaccg acaggcaggc cgcgccgtgg cgctcgaaac tcgcggcgat
     1681 cgctccggga tcgaagttct cgcgcaacac gccccggctc gggctcgctt tcttgatctc
     1741 cgcaataacg gccggcttgc ccgccgtgat tttcgagcgg agcgcaccga tgaagtcgcg
     1801 cgccggcgcg acccgccgcg cctcatcccg cagcgcgccg ggcggtttcg cgcccttcgc
     1861 gcgcgcaacc tccttggcct tcaccgcgag gatgcgctgc aggacgtcag ccattggctt
     1921 gcgtctgcgt gaagcccacg aaagcgtcga gcttcttgcg cggcgcggtg cttgccatca
     1981 ccgccagggc cttctgcacg cctcccgcca gcgtcggcgc gagtcccgcg acgtaaatgc
     2041 cggcgccggc gttcaacgcg acgatatcgc gcgcggggcc cggctcgttg ttgagcgcgg
     2101 ccagcaccag tgccttcgac tgctcgacgc tttccacccg gaccgcggag ccgccgtgcg
     2161 tcttcagccc gaaatcctgc ggcgcgatgt tgtactcgct gatcttgccg tccttgagct
     2221 ccgacaccat cgtcgggccg gcgatcgaaa tttcatccat cccttccagc ccgtgcacga
     2281 ccatcacgtg ccggctgccg agcctttgca gcacgcgcgc ctggatgccg acgagatcgg
     2341 ggtggaacac ccccatcagc tccagccggg cgcctgccgg attggtgagg ggccccagga
     2401 tgttgaagat ggtcttcacg ccgagttccc gcctcaccgg ggcggcatgc ttcatcgcgc
     2461 cgtggtgatt cggcgcgaac atgaagccca ccccgacctc ctcgatgcag cggccgattt
     2521 gctccggctt caggttgatg ttcgcgccga gcgcctccag cacgtcggcg ctgcccgatt
     2581 tgctggaaac cgagcggttg ccgtgcttcg cgacacggcc gccggccgcg gcgcagacga
     2641 aggtcgccgc ggtcgaaatg ttgaacgtgt gcgcgccgtc gccgccggtg ccgacgatgt
     2701 cgatgaggct gtcggaatcc ctgatcggca ccttggtggc gaattcgcgc atgacttccg
     2761 cagcggcggc aatctcgccg atggtttcct tcttgacccg caacccgatg atgatggcgg
     2821 cgatcagggt cggcgagacc tccccgctca tgatggcgcg catgagcgag agcatctctt
     2881 cacggaagat ctcgcggtgc tcgatcacgc gggtcagcgc ttcttgcggt gtcatggttt
     2941 tcctccctcc aggaaattcc ggagcaaatg gtgaccgtgc tcggtaagga tggactcggg
     3001 atggaactgc acgccctcga ccgcgagttc cttgtggcgc acgcccatga tctcgccgtc
     3061 atcggtccag gcgctcacct cgaggcagcc ggggaggccc gcccgctcga tcaccagcga
     3121 atgatagcgc gtcgcggtga aggcatccgg cagtccccgg aacacgccgc ggccgtcgtg
     3181 acgtatggcg gaagtcttgc cgtgcatgag ctgcttggcg tgcacgattt tgccgccgaa
     3241 cgcctggccg atcgcctggt ggccgaggca cacgcccaga atcggaatcc tgccggcgaa
     3301 ttccttgatg agcggcacgg agatgcccgc ctcgttcggc gtgcacggtc ccggtgaaat
     3361 gacgacgtgc gcgggtctga gcttcccgat gccctcgagc gtgatctcgt cgttgcggca
     3421 caccttcacc tcctgcccca gctcgcccag atactgcacc aggttgtagg tgaaggaatc
     3481 gtagttgtcg atcatcagca gcatgatttt caccacagag gcacagagaa cactgaggaa
     3541 actaaaattt cattctcctg gtcccgtgtt tcattacagt cactgtgcct ctgtgtctct
     3601 gtggttcaag gttctaatcc atgcgtgtgt cgaggccgga ctcggccatt tccgccgcgc
     3661 gcaggagcgc tctggccttg ctctgggttt cctgccattc ggcagcgggc tgggagtccg
     3721 ccacgatgcc gccgcccgcc tggacataga gcgtgccgtt cttgacgatc gcggtgcgca
     3781 gcgcgatcgc cacgtccatg ttgccgtgaa agccgaggta gccgaccgcc cccgcgtaga
     3841 tgccgcgttt cacggtctcc agctcgtcga tgatctccat cgcccgcacc ttgggagcgc
     3901 cggagacggt gccggcgggg aaggtcgcgc gcagcacgtc gatcgcgttg agcccgggcc
     3961 tgagcctgcc ttccacgttg gagacgatgt gcatcacgtg cgaatagcgc tcgaccacca
     4021 tttgctccgt gaccctgacc gttccggtct gcgccacgcg gcccgcgtcg ttgcggccga
     4081 gatcgaccag catgatgtgc tccgcgcgct ccttctcgtc cgcgagcaac tccttttcga
     4141 gcgcggcgtc ttcggcgggc gtcgcgccgc gccggcgcgt gccggcgatg gggcgcaccg
     4201 tcaccaaatc gccctcgagg cgcgccagga tttcgggcga ggccccgacc acatggaaac
     4261 cgtcgaaatc aaaataatac atgtagggag aagggttgat ggtgcgcagc gctcggtaga
     4321 gcgcgagcgg cgaggcgcga aacggtttgg acaggcgttg cgaaggcacg acctgcatga
     4381 tgtcgccctc gaagatgtaa cgcttcgcgc gctcgaccgc cttgtgatag gcggattcac
     4441 cgaagcccga gaccgcgggt tcggagggcc cgcgcgcgtc aacggggatc gccaccggct
     4501 cgcgcaattt tgcgagcagc tgcttgaggc gtgcgcgtgc cgactggaac gcgtcacgct
     4561 tgcccggctc ggcgtagacc acgagggtga gcttgcccga caggttgtca acgatcgcga
     4621 tctcttcgga gacgagcagc aggatgtcgg gggccccgat ctcgtcctgc ttccggctcg
     4681 aggcgagccg ccgctcgatg tagcgcaccg tgtcgtaacc gaagtagccg acgaggccgc
     4741 cgcaaaagcg cggcaggctc gcatcctgcg ccaccttgaa acgggcaagg taatcgttga
     4801 cgtaggccag gggatcggaa acccgctttt ccccgaccag ctgcccgtcg cggacctcgg
     4861 cgcaggtttc cccgcgcacc tcgatccgcg tttgcgccgg cagcccgatg aacgagtaac
     4921 ggccgaagcg ctcgccgccc accaccgact cgagcaggta ggagtggcgc cggttggcga
     4981 gcttgaggta gatggaaagc ggcgtgtcga gatcggcaaa ggtctcgagc acgatcggcg
     5041 cgcggttgta gcccgcggcg gcgagccggt tgaattcagc ttcggtcatc agtcacccca
     5101 caaaacggaa aacggtcgaa aaacgcgagc gcgcaaggcg cgc
//
LOCUS       DBNGWZ010000328         3052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2053222, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000328 DBNGWZ010000000
VERSION     DBNGWZ010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2053222"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC16CX_09450"
     CDS             complement(<1..747)
                     /locus_tag="AC16CX_09450"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3012774.1"
                     /translation="MTGAPPDTVRIACLQTNSGNDFAANLATLAAMTREAAAGGAKFV
                     FSAEYVLMMDGSGRTMRENALEPDAAPALSPLTALSRELGVWQLVGSLTLKSDDGRLF
                     NRSVLISAEGRVVATYDKIHMFDATLPSGTVIRESSAYRPGERAVIAGTPWGRLGMTV
                     CYDLRFPQLYRALAQGGAAILAIPSSFQRETGKAHWHVLLRARAIENASFVIAPALCG
                     DHPGKRMTYGHSLVVDPWGEVLADGGEAPGV"
     gene            complement(765..1373)
                     /locus_tag="AC16CX_09455"
     CDS             complement(765..1373)
                     /locus_tag="AC16CX_09455"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0042178 - xenobiotic catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HIR3012775.1"
                     /translation="MRLWFNPASPFARKVRVAARETGLAGRIEEVNAVVSPVKPNTDL
                     ARENPLIKIPALATPDEGTLYDSAVICEYLDSLHQGAPLFPETGPERWRALRLQALGD
                     GMLDAAVLRRYELAVRPQPLQWSDWLAGQNEKIRIGLDRLERECAGWGERFAIGQITA
                     ACVLGYLDFRFPAEGWRASHPELEKWYGLVSQRPSMKATEPK"
     gene            complement(1396..2388)
                     /locus_tag="AC16CX_09460"
     CDS             complement(1396..2388)
                     /locus_tag="AC16CX_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012776.1"
                     /translation="MENPVRHNVSAVLTGTLLAFAATASAWAAEAYPAKPIRLIVGFP
                     PGGANDLVARAVAPRLGARLGQQVIVENRAGAGGNIATELVARAVPDGYTLLLASVAS
                     FAMSPALPGKVPYDPINDFAPVTQAALVTSLLSVHPSLPPRSLKQFVALAKKAPGKIN
                     YATPGAGSIAHLSAELFWHTAGIKLTHVPYKGGGPAVVDAVAGHVESIFSLISTQTPH
                     VRAGKLHALAVSSAKRSVALPDVPSIAESGYPGFEASGWLGLAFPARTPKAIVDRIHK
                     ETAAVLGLREVQSQLEDLGLDAEASDPGAFHARIKSDHARWGKLIREAGIKGGE"
     gene            2525..2992
                     /locus_tag="AC16CX_09465"
     CDS             2525..2992
                     /locus_tag="AC16CX_09465"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR3012777.1"
                     /translation="MSGVTLEQSERIIDAVLARGRELDCRPLSVVVTDPGAHVKAFKK
                     EDGSAMMRFEMAVGKAYAALALGRSSSLVRVRAEERPLFMNYLINASGGRIFPEGGGR
                     LIRNERGEVIGAVGVTGDTEARDEELAAHGIRAAGLKTDEDCARLGKQVRLKN"
BASE COUNT          524 a         1048 c          961 g          519 t
ORIGIN      
        1 cacgcccggc gcctcgcccc cgtcggcgag cacttcgccc cacgggtcca ccaccagcga
       61 atggccgtag gtcatgcgct tgcctggatg gtccccgcac agggccggcg cgatgacaaa
      121 gctcgcgttt tcaatcgccc gcgcgcgcaa cagcacgtgc cagtgcgcct ttccggtctc
      181 gcgctggaac gacgaaggta tcgccagtat cgccgcgccg ccttgggcca acgcccggta
      241 cagctgcgga aaccggaggt cgtagcacac cgtcatcccg agccgtcccc aaggcgtccc
      301 cgcaatcacc gcgcgctcgc ccggccggta ggcactggat tcgcggatga ccgtgccgct
      361 gggcagcgtc gcgtcgaaca tgtggatctt gtcgtaagtg gcgacgacgc ggccttccgc
      421 cgagatcagg accgagcggt tgaacaggcg gccatcgtcg ctcttgagcg tcagcgagcc
      481 cacgagctgc cacaccccca gctcgcgtga aagggcggtg agcggggaaa gcgcaggcgc
      541 tgcgtccggc tccagcgcgt tctcgcgcat ggtgcgcccg ctgccgtcca tcatcagcac
      601 gtattccgcg gaaaagacaa acttcgcccc gccggcggcc gcttcccgcg tcatcgcggc
      661 gagcgtggca aggttcgccg cgaaatcgtt gcctgagtta gtctgaaggc aggcgatacg
      721 gacagtgtcc ggcggggctc cagtcacgag tcaccagtca cgagtcactt gggttcagtg
      781 gccttcatcg acggccgctg cgaaaccaat ccgtaccatt tctccagctc cggatgcgac
      841 gcgcgccagc cctcggccgg aaagcgaaaa tcgagatagc ccagcacgca ggccgcggtg
      901 atctgtccga tggcgaagcg ctcgccccag ccggcgcact cgcgttccag cctgtccagt
      961 ccgatcctga tcttttcgtt ctggccggca agccagtcac tccattgcag cggctgcggc
     1021 ctcaccgcca gctcgtagcg tctcagcacc gccgcgtcga gcatgccgtc acccagggcc
     1081 tgcaaccgca gcgcccgcca ccgttccggt cccgtctccg gaaacagcgg cgcgccctgg
     1141 tgcaggctgt cgagatattc gcagatcacc gccgaatcgt agagcgtgcc ctcgtcgggc
     1201 gttgccagag ccggtatctt gatcagggga ttttcgcgcg cgagatccgt attgggcttg
     1261 accgggctca caaccgcatt gacctcctcg atgcgtccgg ccaggcccgt ttcgcgggcg
     1321 gcgacgcgca ccttgcgcgc gaacggcgat gccgggttaa accacaatcg catggcgcgc
     1381 cccgcggtca cggtctcatt ctcctccctt gatgccggct tcccggatca gcttgcccca
     1441 tcgcgcatgg tcggacttga tccttgcgtg gaaagcgccg gggtcgctcg cctccgcatc
     1501 cagtcccagg tcttcgagct gggactggac ctcgcgcagg ccgaggaccg cggccgtctc
     1561 tttgtgtatt cggtcaacga tcgccttggg tgtccttgcc gggaacgcca gtccaagcca
     1621 gccgctcgct tcgaaccccg gatagccgga ttcggcaatg ctggggacat ccggcaacgc
     1681 aaccgaacgt ttggcgctgc tgacggcgag cgcatgcagc ttgccggccc tgacatgcgg
     1741 agtctgcgtc gagatcaatg aaaagatgct ctcaacgtgg ccggccaccg catcgaccac
     1801 agcgggtccg ccgcctttgt agggaacgtg agtcagcttg atgcccgccg tgtgccagaa
     1861 cagttcggcc gaaaggtgcg caatgctgcc ggcgccgggg gtggcgtaat tgatcttgcc
     1921 gggcgccttc ttcgccagcg ccacgaactg cttcagcgat cgcggcggca gcgacgggtg
     1981 aaccgacaag aggcttgtca ccaatgccgc ctgcgtgacc ggcgcaaagt cgttgatcgg
     2041 atcgtacggc accttgccgg gcagggctgg actcatcgca aaggaagcca ccgacgcgag
     2101 cagcagcgtg tagccgtccg gcacggcgcg cgcgaccagc tcggtggcga tattgccccc
     2161 cgcgcccgcg cgattctcca cgatgacctg ctgcccgagc cgcgcgccca gccgcggcgc
     2221 caccgcgcgc gcgaccagat cgttggcgcc gcccggcggg aaaccgacga tcagccggat
     2281 cggcttcgcc ggataggctt cagccgccca agcacttgcc gttgcagcaa acgccagcaa
     2341 ggttcctgtc aagacggcgg acacgttgtg tcgcacaggg ttttccatca tttgactcct
     2401 ccttgattat tttttgtgat catgggggtc attttatagg aaacgcgttt gaattttccg
     2461 ggcggcgatt taccattaag ccaccgcatg aaatcgtatc gggaaaattc gaggggaggt
     2521 tgaaatgtcc ggggttacgc tggagcaatc cgagcgcatc atcgacgctg tgctcgcccg
     2581 cggccgcgag ctcgactgcc ggccgttgag cgtcgtggtc accgaccccg gcgcccacgt
     2641 caaagccttc aagaaggagg acggctccgc aatgatgcgg ttcgagatgg ccgtgggcaa
     2701 ggcctatgcc gcgctcgcgc tcggccgctc ctccagcctg gtgcgggtcc gcgccgagga
     2761 gcggccgctg ttcatgaatt acctgatcaa cgcgtccggc gggagaatct tcccggaagg
     2821 cggcggccgg ctgatccgga acgaacgcgg ggaggtcatc ggcgccgtcg gcgtcaccgg
     2881 cgacaccgag gcgcgggatg aagaactcgc ggcgcacggc atccgcgcgg ccgggctcaa
     2941 gaccgacgag gactgcgcgc gcctcggcaa acaggtccga ctgaagaact aacctcacgc
     3001 cgcggaggaa tgatcctcca tccctcatcc ctcatccctc atccctcatc cc
//
LOCUS       DBNGWZ010000329         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2431287, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000329 DBNGWZ010000000
VERSION     DBNGWZ010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2431287"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16CX_09470"
     CDS             complement(<1..719)
                     /locus_tag="AC16CX_09470"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR3012778.1"
                     /translation="MTATAAPAPRARAVIDGLYYLTAVFFFVYLLVYYWTSEGGPTLL
                     AITLVPVTFILLTLDELRKNEFYPRLPAVAGYVVAAVYVVISIAVAVYMFVEYEEIGT
                     VRAGIWSAPDLTMGALMVVLIMEYTRKRHMPLFVLNVLLILYAVYGYVVPGMFFHPGL
                     SWERIASATSVEMSTGIFSNLPQLALTLIGSFILVLATLRAFGCVDSILKASKQIAVR
                     SPHALPQSAVLGSMAVGTVSG"
     gene            complement(798..>1604)
                     /locus_tag="AC16CX_09475"
     CDS             complement(798..>1604)
                     /locus_tag="AC16CX_09475"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3012779.1"
                     /translation="GYATGQFEGYYGADIAFYELANDLNRFKGFKASMKRQPVQSFWL
                     FTTEVGTAVRASDRDKFKKWADLSGKPVFTGPLPWDVRAHLERAYNALGVKHQYRQVD
                     LSAAGSLLQSGGIEGFIIYTNGEATTAPWITEASLATDWAALNPSAAELDALKKAGFA
                     ITEVKPDAFKKDVHADKVVLLPFYYGFHVGLEIPEDDVYQMLKVIEKNLPDLVKSDPG
                     YAQVAKDMPGFQRLGVMSSVNLVPVHPGLAKYMREKGVWDAKWDARIAKK"
BASE COUNT          312 a          491 c          524 g          277 t
ORIGIN      
        1 ctgccgctca cggtgccgac ggccatcgag ccgagcacgg cggactgggg cagggcgtgc
       61 ggcgaacgta cggcgatctg cttggaggct ttaaggatcg agtccacgca gccgaaggcg
      121 cgcagcgtgg cgagaaccag tatgaacgag ccgatcagcg tcagcgcaag ctggggcagg
      181 ttggaaaaga tgccggttga catctcgacg ctcgtcgcgc tggcgatgcg ctcccaggat
      241 agcccgggat ggaagaacat gccgggcacc acgtagccgt acaccgcgta gaggatcagg
      301 agaacgttca ggacgaacaa gggcatgtgg cgcttgcgcg tgtactccat gatcagcaca
      361 accatcaacg cacccatggt gaggtcgggc gcgctccaga tcccggcgcg caccgtgccg
      421 atttcctcgt attcgacgaa catgtacacc gcaacggcga tcgagatgac gacgtagacc
      481 gcggcgacaa cgtagccggc gacggccggc agccgcgggt aaaactcgtt cttgcgcagc
      541 tcgtccagcg ttaacaggat gaaggtgacc ggaaccagcg tgatcgcgag cagggtcggc
      601 cccccctcgc tggtccagta ataaaccagg agatagacga agaagaagac tgcggtcagg
      661 taatacagcc cgtcgattac ggcgcgtgcc cggggggcag gcgcggcggt ggcagtcatg
      721 gtcggagtcg ggtccctacg aacagcgggg ggtgagcgca tgcgagcgcc cacccccctc
      781 gattcaccag gccagtccta cttcttcgcg atccgggcgt cccacttcgc gtcccacacg
      841 cccttctcgc gcatgtattt cgccaggccc gggtgaaccg gcaccaggtt caccgacgac
      901 atgacaccca ggcgctggaa tccgggcatg tccttggcga cctgcgcgta acccggatcg
      961 gacttgacca gatcggggag attcttctcg atcaccttga gcatctgata cacgtcgtcc
     1021 tcggggatct cgagcccgac gtggaagccg taataaaacg gcaacaggac caccttgtcg
     1081 gcgtgaacgt ccttcttgaa tgcgtcgggt ttgacttcgg tgatcgcaaa gccggccttc
     1141 ttgagggcgt ccagctccgc ggcgctcgga ttgagtgcgg cccaatcagt cgcgagcgac
     1201 gcttcggtga tccagggcgc cgtcgtggcc tcgccgttgg tgtagatgat gaacccttcg
     1261 atgccgccgc tctggagcag cgatccggcg gcggacaggt cgacttggcg gtactggtgc
     1321 ttcacgccga gcgcgttata cgcgcgctcg agatgagcgc gcacgtccca gggcagcgga
     1381 ccggtaaaca cgggcttccc ggacagatcg gcccattttt tgaatttgtc cctgtcactc
     1441 gcgcggaccg cggtaccgac ctcggtcgtg aatagccaga aggactgcac cggctggcgc
     1501 ttcatgctgg ccttgaagcc cttgaaacga ttgaggtcgt tggcgagctc gtagaaggcg
     1561 atgtcggcgc cgtagtagcc ctcgaactgg cccgtggcgt aacc
//
LOCUS       DBNGWZ010000330         2194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2053438, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000330 DBNGWZ010000000
VERSION     DBNGWZ010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2053438"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="AC16CX_09480"
     CDS             <1..1104
                     /locus_tag="AC16CX_09480"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3012780.1"
                     /translation="VDCHMVRQPVGVCAGITPFNFPVMVPLWMFPVAIACGNAFVLKP
                     SEKVPSASVRMAELFREAGLPDGVLNVVHGDKEAVDAILNHPGINAVSFVGSTPIARY
                     IYETCAKNAKRVQALGGAKNHAVVLPDADLEFAADALTGAAYGSAGERCMAISAVVAV
                     GAAADPLVALLKKKAEAVMVGPGEKEGVDMGPLITRQHRDKVAGYIDQGVKEGAKLVV
                     DGRAHKVPGHEGGFFLGTTLFDNVTPEMKIYRDEIFGPVLVVLRAKTLEDAIALVNRN
                     PYANGTAIFTESGGAARRFENEIQVGMVGVNVPIPVPVAFYSFGGWKSSLFGDLHVHG
                     MEGVKFYTRTKTITTRWPHQDTPAPGMNMPTLG"
     gene            1216..1863
                     /locus_tag="AC16CX_09485"
     CDS             1216..1863
                     /locus_tag="AC16CX_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3012781.1"
                     /translation="MSARESILARIRARQGKSAVPDASEREAVRSHIAARLQSPRPRM
                     DWDPVVRFRERALGLASTMDEVETLTAVPAAVARYLRASSLPLSAVCWTEFENLDWRA
                     AGVEIAVREARESDLVGITGAFCAIAETGTLMTVSGPRTPAAASLLPETHIAIVSASR
                     IVRGMEEAWQLMRDEIVAPPRAVNFISGPSRTADIEQTVTLGAHGPYRVHILLTP"
     gene            2085..>2194
                     /locus_tag="AC16CX_09490"
     CDS             2085..>2194
                     /locus_tag="AC16CX_09490"
                     /inference="COORDINATES: protein motif:HMM:NF039842.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HIR3012782.1"
                     /translation="MTPGKEHPQPFVIKDCALIQIATGVRAQNVRELRDRL"
BASE COUNT          382 a          738 c          743 g          331 t
ORIGIN      
        1 gtggactgtc acatggtgcg ccagccggtc ggcgtctgcg ccggcatcac gccgttcaac
       61 ttcccggtga tggtgccgct gtggatgttc ccggtcgcga tcgcctgcgg caacgcgttc
      121 gtgctcaagc cttccgagaa agtgccgtcc gcctccgtgc gcatggccga gctgttcagg
      181 gaagcggggc tgcccgacgg cgtgttgaac gtcgtgcacg gcgacaagga ggcggtggac
      241 gcgatcctca accatcccgg catcaacgcg gtctccttcg tcggctcgac gccgatcgcc
      301 cgctacatct acgagacctg cgcgaagaac gccaaacgcg tgcaggcgct gggcggggcc
      361 aagaaccacg cggtggtgct gccggacgcc gacctcgagt tcgcggccga cgcgctgacc
      421 ggggcggcct acggctcggc gggcgagcgc tgcatggcga tctcggcggt ggtcgccgtg
      481 ggcgcggcgg ccgatcccct ggttgccctc ctcaagaaga aggcggaggc ggtcatggtc
      541 ggcccggggg aaaaagaggg cgtggacatg gggccgctca tcacgcgcca gcaccgcgac
      601 aaggtggcgg gctacatcga tcagggcgtc aaggagggcg cgaagctcgt cgtggacggc
      661 cgcgcccaca aggtcccggg gcacgaagga ggcttcttcc tcggcacgac gctgttcgac
      721 aacgtgacgc ccgagatgaa gatctaccgg gacgagatct tcggcccggt gctcgtcgtg
      781 ctgcgcgcca agaccctcga ggacgcgatc gcgctcgtca accgcaaccc ctacgccaac
      841 ggcacggcga tcttcaccga gtcgggcggg gcggcacgcc gcttcgagaa cgagatccag
      901 gtcggcatgg tcggcgtgaa cgtgccgatc cccgtgccgg tggcgttcta ttcgttcggc
      961 gggtggaaat cgtcgctctt cggcgatctt cacgtgcacg gcatggaggg cgtgaagttc
     1021 tacacccgca ccaagacgat caccacgcgc tggccgcatc aggacacgcc ggcgccgggc
     1081 atgaacatgc ccacactcgg ctagcgcccg atgcgttagg agtgaggcgt aaggagtgag
     1141 gcggacatcg ataaatcgtc caggcgacgg tgtgaattgc ttcctcacgc ctgacgcctc
     1201 acgcctcacg cattcatgag cgcacgcgaa agcattctgg cgcggatccg cgcgcggcag
     1261 ggaaagagcg ctgtgccgga cgcctccgag cgcgaagccg tacgctcgca cattgccgcg
     1321 cgcctgcaga gcccgcgccc gcgcatggat tgggaccccg tcgttcgctt ccgcgagcgc
     1381 gcgctggggc tggcgagcac gatggacgaa gtggaaacgc tcacggccgt gcccgccgcc
     1441 gttgcgcgct acctgagagc cagttcattg ccgctctccg cggtgtgctg gacggagttc
     1501 gagaacctcg actggcgcgc ggcaggggtc gagatcgccg tgcgcgaggc gcgtgagtcc
     1561 gacctggtcg gcatcaccgg ggcattctgc gccatcgctg aaaccggcac gctgatgacg
     1621 gtcagcggtc cgcgcacgcc ggccgcggcg agcttgctgc cggaaaccca catcgcgatc
     1681 gtctccgcct cgcgcatcgt gcgcggcatg gaagaggcgt ggcagctcat gcgcgatgaa
     1741 atcgtagcgc ccccgcgcgc cgtcaacttc atctccggcc cctcgcgcac cgccgatatc
     1801 gagcaaaccg tcacgctcgg cgcacacggg ccgtatcgcg tgcacatcct gcttacgccc
     1861 tgaaagatca acggcttgcg ctgcaccgtg gcggggcggg aacttttcgc cgcggcgctg
     1921 tcagaaaacc tgataaaaca ggcaattcca taccgtcccg cgcgctaacc gaggacgccg
     1981 cgggctgacc acaagaaaca aaccatcaca ggggaccgcg cccaccgggc gcggggcgct
     2041 ttcttgcgtc cgggccgtga agcggtgcaa aaccggagga ggccatgact cccggcaagg
     2101 aacatcccca acctttcgtc atcaaggact gcgcgctgat ccagatcgcg accggcgtgc
     2161 gcgcgcaaaa cgtgcgcgag ctgcgcgacc gcct
//
LOCUS       DBNGWZ010000331         2982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3311700, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000331 DBNGWZ010000000
VERSION     DBNGWZ010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3311700"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16CX_09495"
     CDS             complement(<1..376)
                     /locus_tag="AC16CX_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012783.1"
                     /translation="MSIGTVLLIVLCNMSSFRASKVLVSLFAIELGASQFTIGVMIAM
                     YSLFPALLAVYAGKLSDRLGVRLPMLAGSLGLSAGLLVPWLSSSLPGLYASAMMIGAS
                     HMFYNVSAQNLIGSIGGAEDRTR"
     gene            complement(524..1711)
                     /locus_tag="AC16CX_09500"
     CDS             complement(524..1711)
                     /locus_tag="AC16CX_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012784.1"
                     /translation="MPRAPEPISASTPHRLLSLYEILAIILLWNAGYKGVRVANTLYA
                     LELGASPFDTGILLAFYGLFPLLLAVHIGKLGDRYGVRLPVVAGAIISALGIVLPYFW
                     PVFPMLFVSAAVTGAGFILVQVSMQSLIGSLGSGPARTRNLNLYALIVSVSDLMGPVV
                     AGFSIDHFGHVRTYLVLVLLTLPSVLALVFLGHRFPRTATVGPDRSQHRMKDLFGIRD
                     LRRMLMTSALIMGGLDLFQLYLPIYGHSLALPASTIGVIMGAFAAAAFVTRALLSVLA
                     RRVGEEPALRYAMFLAAGTFFLIPAFENGIVLAAVCFVLGLGMGLGQPLTTILTYNYA
                     PAGRHGESLGLRIAINNTMHVSVPVVFGAVGSLLGLAPVFWASSAMLALGGYTARRSP
                     RDP"
     gene            1762..2718
                     /locus_tag="AC16CX_09505"
     CDS             1762..2718
                     /locus_tag="AC16CX_09505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3012785.1"
                     /translation="MNYNQRMKKESSVEDHSIRQQVLLALASNRTPGFHFPGHLLGCA
                     WPRIGEDDLEQTALDGPQSRNVDGSTSLAAFCVLLDTALATASRLKIERGARQATVHL
                     HAQFTGAPLRGELRAHARMGGYTAGASVRQSMTSGTVYAGGVPMCHASATFVVLPPPP
                     GVNLAPLPWQRESGELASPLNEKELDPDERSIMKACNSALKRANDRHAFIEYFWNIMP
                     SQTDTGARCTVKLGPQHNNRVRHVQGGLLLGLAAITARAAAPQHPQLSAIAAWYISPG
                     QGKALKIRSKRIHEGRSFAAVHTEVRNADGSRVLEVVSHHAA"
     gene            complement(2831..>2982)
                     /locus_tag="AC16CX_09510"
     CDS             complement(2831..>2982)
                     /locus_tag="AC16CX_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR3012786.1"
                     /translation="QYFPKGTDLSVHSQVQLNAVARRLNERPRKTLNYETPAERFAQC
                     VASVG"
BASE COUNT          627 a          933 c          972 g          450 t
ORIGIN      
        1 tgcgggtgcg gtcctcggcg ccgccgatcg agccgatcag gttctgcgcg gatacgttgt
       61 agaacatgtg cgaggcgccg atcatcatcg cggaggcgta gagccccggc agcgaggacg
      121 acagccatgg caccaagagc cccgcgctga ggccgagcga gccggccagc atcggcaggc
      181 gcacgccgag ccggtccgag agcttgccgg cgtacaccgc aagcaacgcc gggaacagcg
      241 agtacatcgc gatcatgacg ccaatggtga actgggaggc gccaagctcg atcgcgaaca
      301 gcgacaccag caccttgctc gcgcggaagc tcgacatgtt gcacagcacg atcaggagga
      361 cggtgccgat cgacatcgct cacgcttcag gaatttgccg ggccgttgtc cggcaaattc
      421 ctaacgagaa attccatgaa ggaaagtagt ctttcccaat aagcccgcgt gcagcaggct
      481 cttccgcgca cgattggcat ttatcgctcc aattccacgc cagtcacgga tcacgcgggc
      541 tgcggcgggc tgtgtaaccg cccagcgcga gcattgccga gctcgcccag aacaccggcg
      601 ccagccccaa cagggagcca accgccccga acaccaccgg caccgatacg tgcatcgtgt
      661 tgttgatcgc gatcctcaag cccaggcttt cgccgtgccg tccggcgggc gcatagttgt
      721 aggtcaggat ggtggtgagc ggctggccga gccccatgcc cagcccgagc acaaagcaga
      781 ccgcggccag cacgatgccg ttttcgaacg cgggtatcaa gaaaaaggtg cccgcggcaa
      841 ggaacatggc gtaacgcaac gccggctcct ctccaacccg gcgggcgagc acggacagca
      901 gcgcccgtgt gacgaacgcc gccgcagcaa acgcgcccat gatcacgcca atggtggaag
      961 ccggcagcgc gagcgagtga ccgtagatcg gcaggtagag ctggaaaaga tcgagaccgc
     1021 ccatgatgag agcgctcgtc atcagcatgc ggcgcaagtc ccggatgccg aacaggtcct
     1081 tcatgcggtg ctggctgcga tcggggccaa ccgtggcggt gcggggaaag cggtggccca
     1141 gaaaaaccag ggccagcacg gacgggaggg tcagcaagac gagaacaaga tacgtgcgca
     1201 cgtggccgaa gtgatctatc gagaagccgg ccacgaccgg ccccatcagg tcggagaccg
     1261 acacgatcag cgcatagagg ttgaggttgc gggtgcgcgc ggggccgctc ccgagcgagc
     1321 cgatcagcga ttgcatcgac acctgcacca ggatgaatcc ggcgccggtg acggcagcgg
     1381 aaacgaaaag catcggaaac acgggccaga agtacggcag cactatcccg agcgcggaga
     1441 tgatggcgcc ggcaacgacg ggcaggcgca cgccgtagcg gtcgccgagc ttgccgatgt
     1501 ggaccgcgag caacagcggg aacaatccgt agaaggcgag caatatcccg gtgtcgaaag
     1561 ggctggcgcc cagctccagc gcgtagagcg tgttggccac gcgcacgccc ttgtagcccg
     1621 cattccacag cagaatgatg gcgagaatct cgtaaaggct gaggagacgg tgaggcgtgg
     1681 aagcggaaat cggttcggga gctcgcggca ttttgagccc tgattttacc gcacatgcgc
     1741 gctacaaaag gcgcggcgcg catgaattac aatcagagga tgaaaaagga atcttcggtt
     1801 gaagatcatt cgattcgcca gcaggtgttg cttgccctcg cgtcgaatcg cacgccgggc
     1861 ttccactttc ccggccatct cctcgggtgc gcctggccgc gcatcggcga ggacgatctc
     1921 gaacagaccg cgctcgacgg cccgcaatct cgcaatgtgg acggctcgac cagtctcgcg
     1981 gcgttctgcg tcctgctcga caccgcgctc gccaccgcat cgcgcttgaa aatcgagcgc
     2041 ggcgcgcgcc aggccaccgt gcacctgcac gctcagttca ccggcgcgcc gctgcggggc
     2101 gagttgaggg cccacgcgcg catgggcggg tacacggccg gtgcgtcggt gcgccagtcg
     2161 atgacgagcg gcacggtata cgccggcggc gtgccgatgt gccacgcgag cgccacgttc
     2221 gtcgtgctgc cgccgccgcc cggtgtgaac ctcgcgccat tgccttggca gcgcgaaagc
     2281 ggcgagctgg cctcgccgct taatgagaaa gaactcgatc cggatgaacg tagcataatg
     2341 aaagcgtgca attcggcgct gaaacgcgcc aatgaccggc acgctttcat tgagtacttc
     2401 tggaacatca tgccgtcgca gaccgacacc ggcgcgcgct gcacggtgaa gctcggaccg
     2461 cagcacaaca accgcgtccg ccacgtgcag ggcggcctgt tgctcgggct cgcggcgatc
     2521 accgcgcgcg ccgcggcgcc gcagcaccct caactctccg caatcgcggc ctggtatatc
     2581 agcccggggc aagggaaggc gctcaagatc cgctcgaagc gcatccacga aggccgcagc
     2641 ttcgcggccg tgcacacgga agtccggaac gcggacgggt cacgagtgct ggaggtcgtc
     2701 agtcatcatg ctgcctgacg acggtgacga gatacgggtg acgagataga ggtgacgaga
     2761 tacggttgac gggtaatgtg aagtagccgc gacttaatct aaaggcgagt gtccgataac
     2821 ggctgcggat tcaaccgacc gacgcaacac attgtgcaaa tcgttcggct ggtgtttcgt
     2881 agtttagcgt tttcctcggc cgttcattta accgcctggc caccgcattc agttgcacct
     2941 gcgagtgaac cgagagatcc gtccccttcg ggaagtactg tc
//
LOCUS       DBNGWZ010000332         7344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3440254, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000332 DBNGWZ010000000
VERSION     DBNGWZ010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3440254"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16CX_09515"
     CDS             complement(<1..271)
                     /locus_tag="AC16CX_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3012787.1"
                     /translation="MKPTNLLIIMSDEHNPKVMGCAGHPIVKTPNIDALAARGTMFSS
                     AYTTCPVCVPARAAFAVGKYVHQIGFWDNADAYDGSIPSWHHRLRE"
     gene            complement(268..1086)
                     /locus_tag="AC16CX_09520"
     CDS             complement(268..1086)
                     /locus_tag="AC16CX_09520"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3012788.1"
                     /translation="MDTPFLIASGPEILTRLLVRAVPSLPKYAQLRDTLLAAIEGGYW
                     KPGAKLPTEQELARTMPFSLGTVQRALRALVDEGIVVRLQGSGTYVAKGRKPMDAPWH
                     CRFLDDSGRGFLPIYPKVVARRRIAERGSWSVHLAPDGGDAIRIDRVINVNDEFQVYS
                     QFYLDAGRFASMLTRPAAELDGANLKIIISREFGLPVTQVAQTMSTVRFPDAVRRALK
                     LRRAVTGTLLEIAASAGRDRPLYYQELYIPPNWRRLVISDTVPGQSISRRSPTK"
     gene            complement(1086..1988)
                     /locus_tag="AC16CX_09525"
     CDS             complement(1086..1988)
                     /locus_tag="AC16CX_09525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012789.1"
                     /translation="MAATITKDAPIGFIGLGVIGGAMARNLLKADFKVTVHNRSRGKV
                     EELVRDGAKDGGSPTGVARASDVVLLCLPDTPDVERVLFGDEGVVGGIKRDAVVIDCS
                     TISATATVEFARRLREHGAHMLDSPVSGGPKGAQDGTLSCMIGGEAAIVERCMPVLQA
                     IGKTFTRIGGNGSGQACKSCNQLVLTGTLMAVSEAFALAKKLGIDPNKVRDALLGGSA
                     QSFVLANHGKRLLDGTLQPGFRAGLLLKDIKLAQSTGRDAGTFMPMTALGLQLLTALC
                     NSGRDSLDSAALGLLFEEFSGIKR"
     gene            complement(2075..3058)
                     /locus_tag="AC16CX_09530"
     CDS             complement(2075..3058)
                     /locus_tag="AC16CX_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012790.1"
                     /translation="MSAKSMTLNVISAMGLVATFVIAQDAAAQPYPARPIRLIVAWGP
                     GGAPDIFARIVGQKLYEAMGQPVIVDNRPGATGNIGAEIAAKAPPDGHTIFNATLSLV
                     ISPGFYKKLPFDPIASFAPVTMLASVPLILVIHPGLPVKSVTELITLAKSRPGSLNYA
                     SVGIGSPAHVSAELFQIMGGGKLVHVPYKSGGAMATAILTGEAQLSFIAIAPALPHVN
                     ARRLRVLAITSAKRSPAVPDVPTFTEAGVPGIEVDNWNGILTPAGTAKSVVNRLHGEI
                     VKAVRAPEIADQFARQGAEVNTSSPAKFSTIIKAEVTKWAKVVRAAGIKPQ"
     gene            complement(3080..5458)
                     /locus_tag="AC16CX_09535"
     CDS             complement(3080..5458)
                     /locus_tag="AC16CX_09535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012791.1"
                     /translation="MKPANGAVETRPNYIGAPLPRPNAKRLLAGRGQYVDDVRLPRLV
                     HAAFVRSPYAHARITGVDAREAECSPGVVRVVTGADVAKLCRPYVGVLAHVRGMRSAP
                     QYPLAVDRARWQGEPVAAVVAETRAQAEDAAQLVKIEWDELPAVTDPETALDPATPAI
                     HPDLGGNLCFERVVDTGGVDEAFTRAHKVVEATFVTGRHTAVSLEPRAILADYNSADH
                     HLTVWHSTQVPYMMQWIIARHFGLPESAVRVIAPDIGGGFGLKIHIYGEDMATIAAAM
                     LTGRPVKFVADRLESFVGDFHARGHRVKARIAVSRNGDVTAIEMDDLYGIGPYSAYPR
                     GSANEGIQVVNLTGGPYRNRIYRGRTRAAFQNKPMYGQYRAVGHPIVCAVTEGLVDLA
                     ADALGMDPAEFRRRNYIAPDAYPYTLPSGLVFEKLSQHEALEKLLAMMGYQRLRHEQA
                     ELRNQGVYRGIGLASFVENSNPSSATYGQGGVSIASQDACTIKLTATGGITVAASVNE
                     IGQGAHTIAAQIAATALGVPIERVKMILGDTDATPYGGGNWGSRGTGIGGEAVLQAGK
                     ALRSNILSFVARLAGTEASLLDIRDGNVVEKASGAPCMTLEEVARTAYFRTERVPADF
                     QPELTVTRSYAQKAYTGVYTNGIQASYLEVDVETGFVKLLGHWVVDDCGTIVNPLLAD
                     EQIRGAVVQGIGAALYEECIYSPEGQLLNGSLMDYLVPMAGEMPDIEVAHTCTPTATS
                     ELGAKGAGEAGTAGAPAAVMNAINDALKPFGARVFEQPFTPGRILRALGKVG"
     gene            complement(5455..5952)
                     /locus_tag="AC16CX_09540"
     CDS             complement(5455..5952)
                     /locus_tag="AC16CX_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012792.1"
                     /translation="MTRPAEFVDIALSVNGAQVARRVAARQHLVDFLREELGLTGSHL
                     GCEHGTCGACTVRVNGEIVRGCLMFAAQCDGAHVETIEGLSDDGTLADLQRAFHERNA
                     LQCGFCTAGFLIAALELIERNPHARRAEIREHLSGNYCRCTGYQSIVEAVEETCRARV
                     SGETA"
     gene            complement(5962..6819)
                     /locus_tag="AC16CX_09545"
     CDS             complement(5962..6819)
                     /locus_tag="AC16CX_09545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3012793.1"
                     /translation="MKAPAFAYVKPALLAEALELLERYGDGAKILAGGQSLLPALNMR
                     LATPRVLIDINGLTELAGIRVAEDKLVIGALTRHRVVERSSEVARCAPLIHQAMRHVA
                     HAAIRNRGTFGGSIAFADPAAELPACCLALGAEFVIASTGGERRVAARDFFKGLYDTD
                     LRPGEVLLAGEIPVIRPGYRSVFQELARRRGDYAIVGLAAHGRVEDGVLSDLALAFFG
                     VGATSVAARHAARAIEGKMFSAAAVAVAQAALDRDLAPLDDIHHSAAAKLHLARVLLG
                     RALAALAQR"
     gene            complement(6948..>7344)
                     /locus_tag="AC16CX_09550"
     CDS             complement(6948..>7344)
                     /locus_tag="AC16CX_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3012794.1"
                     /translation="CFFGELIALGMKSRGCAGALVDGGIRDIEWIAKQQFPVYARYCT
                     PVQSIGRWKVTAWQVPAYLPGATKQHVIVNPGDFVLGDTDGVVVVPADAVEKALNEAE
                     RLTATEVKIRAELNAGATLEQVLAKYGHV"
BASE COUNT         1219 a         2482 c         2328 g         1315 t
ORIGIN      
        1 tttcgcgcag gcgatggtgc cagctcggga tcgagccgtc gtaggcgtcg gcattgtccc
       61 agaagccgat ctggtgcacg tacttgccga ccgcgaaggc ggcgcgcgcc ggcacgcaca
      121 ccgggcacgt cgtataggcg gaggagaaca tcgtgccgcg cgcggcgagt gcgtcgatgt
      181 tgggagtctt gacgatggga tgcccggcgc aacccatgac cttcgggttg tgctcgtcgg
      241 acatgatgat caggagattc gtcggcttca tttcgtcggg gatcgtctgg agatggactg
      301 cccaggaacg gtgtcggaaa tcaccagtcg gcgccagttg ggcggtatgt agagctcctg
      361 gtagtacaag ggccggtcgc gccccgcgct tgcggcgatc tccagcagcg tgccggtgac
      421 ggcgcggcgc agcttgagcg cccggcgcac ggcatcggga aagcgcacgg tgctcatggt
      481 ctgtgccact tgggtgaccg gtagcccgaa ctcgcggctg atgatgatct tgaggttggc
      541 gccgtcgagc tccgcggccg ggcgtgtcag catgctcgca aaacgcccgg catccagata
      601 gaactggctg tagacctgga actcatcgtt gacgttgatg acacgatcga tccggatcgc
      661 gtcaccgccg tccggggcaa gatgaaccga ccaggagccg cgctctgcga tgcgccggcg
      721 cgccaccacc ttgggataga tcggcaggaa tccgcggccg gagtcgtcga ggaagcgaca
      781 gtgccacggc gcatccatcg gcttgcgccc cttggcgaca taggtaccgc tcccctgcag
      841 gcgcaccacg atgccctcgt ccacgagcgc gcgcagcgcc cgctgcacag tgcccaggct
      901 gaaaggcatg gttcgcgcaa gttcctgctc ggtcggcagc ttcgcacctg gtttccagta
      961 gccaccctcg atcgcagcga gcagcgtgtc gcgcagctgc gcgtacttcg gcaggctcgg
     1021 caccgcgcgc acgagcagcc gcgtgagaat ctcgggaccg gatgctatta agaacggcgt
     1081 atccactatc gcttgatacc cgagaactct tcgaatagca gcccaagtgc tgcgctatcc
     1141 agtgaatcac gcccgctgtt gcacaaggca gtcagaagct gaagtccgag cgcggtcatt
     1201 ggcataaagg ttcccgcatc gcgcccggta gattgtgcga gcttgatgtc cttcagtaga
     1261 agccctgcac ggaaccctgg ctgcaacgtg ccatctagaa gccgtttccc atggttggcc
     1321 agcacaaagc tctgcgccga accgcctaac agcgcatccc gcaccttgtt cggatcgata
     1381 cccagtttct tcgcaagggc aaaggcttca gagacggcca tcaaggtccc agtcaggacc
     1441 agctggttgc acgatttgca tgcctggccc gatccgttgc ctccgatgcg cgtaaaggtt
     1501 tttccaattg cctgcaacac gggcatgcat cgctcgacga ttgccgcctc gccgccgatc
     1561 atgcaggaga gcgtgccgtc ctgcgctcct tttggcccgc cgctcaccgg cgagtcgagc
     1621 atgtgcgcgc cgtgctccct cagccgccgg gcgaactcca ctgttgcggt tgccgaaatg
     1681 gtgctgcagt cgattaccac cgcgtcccgc ttgatgcctc cgaccacgcc ctcgtcccca
     1741 aagaggacgc gctcgacgtc gggggtatcg ggcaaacaca acagcacgac atcggacgcg
     1801 cgcgctacgc ccgtcggcga accgccgtcc ttggcgccgt cgcgcacgag ctcctccacc
     1861 ttgccgcggc tgcggttgtg caccgtcacc ttgaagtcgg ccttcaacaa gttacgcgcc
     1921 atcgcgccgc caatgacgcc caggccgatg aagccgatcg gcgcatcttt cgtaatcgtt
     1981 gctgccacgt gtcctcctta gtccggcagg cccggcactg aacgcgccag caactcgacg
     2041 atcctgcaca cagcgatttt ttccctgtgc cagctcattg cggcttgatg ccggcggccc
     2101 tgaccacctt ggcccacttg gtgacttctg ccttgatgat ggtcgaaaat ttcgccgggc
     2161 tgctggtgtt gacctccgcg ccttgtcgcg caaactgatc cgcgatctcc ggcgcacgga
     2221 cggccttgac gatctcgcca tgtagcctgt tgaccacgct cttcgcggtc cccgccggcg
     2281 tgaggatccc gttccagttg tcgacctcga ttcccggcac gccggcttcg gtgaacgtcg
     2341 gcacgtcagg gaccgccggg gaacgcttcg cggacgtaat cgcgaggacg cgcaggcgcc
     2401 ttgcattcac gtgcggcagc gcgggtgcta tggcgatgaa agaaagctgc gcttcgccgg
     2461 tcagtatcgc ggtagccatc gcgccaccgc tcttgtaagg cacgtgcacc agttttccac
     2521 cccccatgat ctggaacagc tctgccgaca catgcgccgg actgccgatg cccaccgaag
     2581 cgtaattgag actgcccggc cgggatttgg cgagcgtgat cagctccgtc accgatttca
     2641 ccggcaggcc gggatggatg accaatatca gcggcaccga ggccagcatg gtcacgggcg
     2701 cgaagcttgc gatgggatcg aacggcagct tcttgtagaa gccggggctg atcaccagcg
     2761 acagcgtcgc attgaaaatg gtatgcccgt caggcggggc cttggcggcg atttcggcgc
     2821 cgatgttccc cgtcgcgccg gggcggttgt ccacgatcac cggctgcccc atcgcctcgt
     2881 agagcttctg tcccacaatg cgggcaaata tgtccggcgc cccgccgggc ccccacgcaa
     2941 caatcagccg aattggcctg gccggatacg gctgggccgc ggcgtcctgc gcgatcacga
     3001 atgtcgccac cagccccatt gctgaaatca cgttcagcgt catgctcttg gcactcattg
     3061 acgttttcct cctcacctgt cagccgactt ttcccagcgc gcgcagaatg cgcccgggcg
     3121 tgaacggctg ctcgaagacg cgcgcgccga acggtttcag ggcgtcgttg atcgcgttca
     3181 tgacggccgc cggtgcgcct gcggtgcccg cctcgcccgc gcccttggcg ccgagttcgg
     3241 aggtcgcggt gggcgtgcag gtgtgcgcga cctcgatgtc cggcatctcc cccgccatcg
     3301 gcacgaggta atccatgagg ctcccgttca ggagctgccc ttccgggctg taaatacact
     3361 cttcgtagag cgccgcgcct atgccctgaa ccaccgcgcc gcggatctgc tcgtccgcga
     3421 gcaaggggtt cacgatggtg ccgcagtcgt ccaccaccca gtgcccgagc agcttcacga
     3481 atccggtttc gacatccact tcgaggtagc tcgcctggat gccgttggta tagacgccgg
     3541 tgtaggcctt ctgcgcatag ctgcgcgtga ccgtgagctc cggctggaag tccgctggca
     3601 cgcgctcggt gcggaaatag gcggtgcgcg caacttcttc cagcgtcatg cacggcgcgc
     3661 ccgatgcctt ctcgaccacg ttgccatcgc ggatgtcgag tagcgaggcc tcggttccgg
     3721 ccagccgggc gacaaagctc aggatgttcg agcgcaaggc cttgcccgcc tggagcacgg
     3781 cctcgccgcc gatgcccgtg ccgcgcgagc cccagttgcc gccaccatat ggcgtggcgt
     3841 cggtgtcgcc caggatcatt ttgacgcgct cgatgggcac gccgagcgcg gtggcggcga
     3901 tctgcgccgc gatcgtgtgg gcaccttgcc cgatctcgtt gacgcttgcg gcgacggtaa
     3961 tcccgcccgt cgcggtgagc ttgatggtgc acgcgtcctg cgacgcgatt gagaccccac
     4021 cctggccgta cgtcgccgaa gacggattgg aattctcgac gaaactcgcg agcccgatgc
     4081 cgcggtatac gccctggttc ctcaactcgg cctgttcgtg gcgcagccgc tggtaaccca
     4141 tcatcgcgag cagcttctcg agcgcctcgt gctgcgacaa tttctcgaat acgagcccgc
     4201 ttggcagcgt gtagggatag gcgtcggggg cgatgtaatt gcgccggcgg aactccgcgg
     4261 ggtccattcc gagcgcatcc gccgcgaggt ccaccagccc ttcggtgacc gcgcacacga
     4321 tggggtgccc gaccgcgcga tactggccgt acatcggctt gttctggaac gcggcgcgcg
     4381 tccgcccgcg gtagatccgg ttgcggtacg gcccgccggt gagattgacc acctgtatgc
     4441 cttcattggc gcttccacgc ggataggcgg agtacggtcc gatgccgtag aggtcatcca
     4501 tctcgatcgc ggtgacgtcc ccattcctcg ataccgcgat ccgtgctttc acccggtgtc
     4561 cccgcgcgtg gaagtcgccg acgaacgact ccaggcgatc ggcgacgaat ttgacgggcc
     4621 tccccgtcag cattgctgcc gctatcgtcg ccatgtcctc gccgtagatg tggattttca
     4681 ggccaaatcc accgccgatg tcgggcgcga tcacccgcac ggccgattcc ggcagcccga
     4741 aatgccgcgc gatgatccac tgcatcatgt acggcacctg cgtcgagtgc cagacggtga
     4801 gatggtgatc cgcggagtta tagtccgcga gaatcgcccg cggctccagc gacaccgcgg
     4861 tatggcggcc ggtgacgaaa gtggcttcca ccaccttgtg cgcgcgcgta aacgcctcgt
     4921 ccacgccgcc cgtgtcgacc acgcgctcga aacataggtt gccgccgaga tccgggtgga
     4981 tggccggggt cgcaggatcg agcgcggtct caggatcggt gaccgccggc agctcatccc
     5041 attcgatctt gaccaattgc gcggcgtctt ccgcctgcgc ccgcgtttcc gcgacgaccg
     5101 cggccaccgg ctcgccctgc cagcgcgcgc gatccaccgc gagcggatac tgcggcgccg
     5161 agcgcatacc cctgacatgc gcgagcacac ccacgtacgg cctgcagagc ttcgccacgt
     5221 ccgcgcccgt gacgacgcgc acgacgccgg ggctgcactc ggcctcgcgg gcgtcgacgc
     5281 cggtgattcg cgcgtgggcg taggggctgc gcacgaacgc cgcgtgcacc agccgcggga
     5341 gccgaacgtc gtccacgtac tgcccacgcc ccgcgagcag tcgttttgcg ttggggcgcg
     5401 gcagtggggc gccaatgtaa ttcggacgcg tctccacggc gccgttcgcc ggtttcatgc
     5461 ggtttccccc gagacacgcg cgcgacacgt ctcttccacc gcctcaacga tggactggta
     5521 gccggtacag cggcagtaat tgcccgacag atgttcgcga atttccgcgc ggcgcgcatg
     5581 cggattgcgc tcaatgagct cgagcgcggc aatcaggaat cccgccgtgc agaaaccaca
     5641 ttgcagggcg ttgcgctcat gaaacgcccg ctgcagatcg gcgagcgtgc catcgtcgga
     5701 cagtccttcg atcgtctcga cgtgcgcgcc gtcgcactgc gcggcgaaca tgaggcagcc
     5761 gcgtacgatt tcgccgttga ctctgaccgt gcatgcgccg cacgtgccgt gctcgcagcc
     5821 caggtgcgaa ccggtcagcc ccagctcttc gcgcaggaag tccacgaggt gctggcgtgc
     5881 cgcaacgcgg cgcgccacct gcgcgccgtt cacgctgagc gcgatgtcga cgaattcggc
     5941 cggccgcgtc atgtcgttcg cctagcgttg cgcaagcgca gcaagcgccc gcccgagcag
     6001 cactcgtgcg agatgcagct tcgccgccgc gctgtggtgg atatcgtcga ggggcgcgag
     6061 atcccggtcc agcgccgcct gcgcgaccgc aactgcggct gcagagaaca tcttgccctc
     6121 gatcgctcgc gcggcatgcc gcgcggcaac ggaggtcgcc cccacgccga agaaggcgag
     6181 tgcgagatcg ctcaggacgc cgtcttctac cctgccgtgc gcggcgagcc cgacgatcgc
     6241 gtagtcgccg cggcgacgcg ccagctcctg aaacaccgag cggtatcccg gcctgattac
     6301 cgggatttca ccggcgagca ggacctcgcc gggcctgagg tccgtgtcgt acaagccctt
     6361 gaagaaatcc cgcgccgcca cccgccgctc gccgcccgtc gaggcgatca cgaactcggc
     6421 gccaagcgcc aggcagcagg cgggcagctc cgctgcggga tcggcaaacg cgatgctgcc
     6481 gccgaaggtg ccgcggttgc ggatcgctgc gtgcgcgaca tgtcgcatcg cctggtgaat
     6541 gagcggcgcg cagcgcgcaa cctcggacga gcgctcgacc acgcggtgac gggtaagcgc
     6601 cccgatgacc agcttgtctt ctgccacgcg gattccggcg agttcggtaa gcccgttgat
     6661 gtcgatcagc actcgaggag ttgcgagccg catgttgagc gccggaagca agctctgccc
     6721 gccggcgagg atcttcgcgc cgtccccgta gcgctcgagc agctcaagcg cttcggcgag
     6781 caatgcgggc ttgacgtagg cgaaagcggg ggctttcact tcctcctcgt gggaatatgg
     6841 acggaacgcc cagcgttttt cagcaaactt cgcgctggtc gctcgaatac tctattgcta
     6901 tatagtattt gccgtcaagc cgccaaagcc tgtggccaaa gagcgaatta cacgtgtccg
     6961 tacttggcga gcacctgttc gagggtcgcg ccggcattga gctcggcgcg gatcttcact
     7021 tcggtagcgg tgagccgttc ggcctcgttg agcgctttct ccaccgcgtc ggcggggaca
     7081 accactacgc cgtccgtgtc gccgagcacg aaatcgcccg gattgacgat gacgtgctgt
     7141 ttggtcgcgc cgggaagata ggctggcacc tgccacgccg tcaccttcca gcggccgatc
     7201 gactgcaccg gcgtgcagta acgggcgtac accggaaact gctgtttcgc gatccactcg
     7261 atgtcacgga tgcctccgtc caccagagcg cccgcgcagc cgcgcgactt catgccgagt
     7321 gcgatcaatt cgccgaagaa gcac
//
LOCUS       DBNGWZ010000333         1518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2180715, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000333 DBNGWZ010000000
VERSION     DBNGWZ010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2180715"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1496)
                     /locus_tag="AC16CX_09555"
     CDS             complement(318..1496)
                     /locus_tag="AC16CX_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_194713029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR3012795.1"
                     /translation="MTRPLFEIADVIRRADARITSRHRESLTWPQVKVLNAILRCRTA
                     ALGGHRDQCTRCGYQAISYNSCRNRHCPKCQTSAREKWLTARQRELLPVDYYHLVFSV
                     PHALVPLIWQNKRVLFRLLFEASAETLLEVAADPKHLGGEVGFLSILHTWGQTLQPHP
                     HVHCVVPGGGLSPDRQRWIRSPPRFFLPVRVLSRVFRGKFVAGLKRAFCRNKLAFHGA
                     YLPLADKITFGKFLRTLFREDWVVYAKRPFGGPEHVLQYLARYTHRVAISNHRLVDLT
                     DSHVTFRWKDYAHHNKRRTMTLTHEVFLRRFLQHVLPRGFPRIRYFGFLANRRRGEFL
                     PLCRTLLAAAPPADVANAAEPAVRRCPCCQGPMRILEFLTAKQVHYEETRQVPVLDSS
                     "
BASE COUNT          316 a          430 c          511 g          261 t
ORIGIN      
        1 gcccggcggg atggagccga ggcgaaggtc gaaaattgaa cgccgggcac ttcggataaa
       61 gtcgtaagaa gtgagccgct ggcgcgtgcg ccgcgaccgc ctcaagctat gggtcttgaa
      121 tggtggcgac tgcgccatgg tcaggcacaa catgaggtct gatgggcctg gatgtgccta
      181 tggttcggtt gaacggcttc gggaacatcg cgccaaacat gtgggcaagc ggcagaaagc
      241 gatgaagtgg gcgatgaaga cacacgtgcc gggggcgtgc cggcgggcaa gcctgatgat
      301 tcggcgaggc gcgtaactta cgaactgtca agaactggaa cctgacgggt ctcctcgtaa
      361 tgaacctgct tggccgtcag gaactccaga atccgcatgg gcccctggca acaaggacag
      421 cgccggaccg ccggttcagc tgcgttcgcc acgtctgctg gcggcgcagc cgccagcaga
      481 gttcggcaga ggggcaggaa ctcaccgcga cgccgattcg cgaggaagcc gaagtaccgg
      541 atgcgcggga agccccgtgg gagcacatgt tggaggaagc ggcgcaagaa gacttcgtga
      601 gtcagtgtca tcgttcgccg cttgttgtga tgggcgtagt ctttccaccg gaacgtgacg
      661 tgggagtcag tgagatcgac gagtcggtgg ttggagatcg cgactcgatg cgtgtaccgg
      721 gccagatact gcaaaacgtg ttcgggaccg ccgaagggac gcttcgcata taccacccag
      781 tcttcgcgaa acaaggtccg caaaaacttc ccgaaggtga tcttgtccgc tagcggaagg
      841 tacgcgccat ggaacgcgag cttgttccgg caaaacgcgc gcttcaagcc ggccacaaac
      901 ttgccacgaa agacgcggct caaaaccctc accgggagaa agaagcgcgg tggcgatcgg
      961 atccaccgtt gccggtccgg ggacaagccg ccgcccggca cgacacagtg aacatgggga
     1021 tgcggctgta aggtctgtcc ccaggtgtgg aggatactca agaagccgac ttcgcctccg
     1081 aggtgcttgg gatcggcggc gacttcgagc agcgtctccg cgctggcctc gaagaggagc
     1141 cggaaaagga ccctcttgtt ctgccagatc agcggtacca gcgcatgcgg cacgctgaaa
     1201 accagatggt agtagtccac gggcagcagt tctcgctgtc gcgccgtcaa ccacttctca
     1261 cgggcactgg tttggcattt cgggcagtgg cgattccggc aggagttgta ggagatagcc
     1321 tgatatccgc agcgcgtaca ttgatcgcga tggccgccga gcgccgccgt ccggcagcgc
     1381 aggatggcat tcagcacctt cacctgcggc cacgtcaagg actcgcgatg gcggctggtg
     1441 attcgggcat cggctctgcg gataacgtcg gcgatctcaa acagcggccg tgtcacggct
     1501 gctgttctcc ggcggctc
//
LOCUS       DBNGWZ010000334         3705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_667409, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000334 DBNGWZ010000000
VERSION     DBNGWZ010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_667409"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16CX_09560"
     CDS             complement(<1..347)
                     /locus_tag="AC16CX_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3012796.1"
                     /translation="MSTSHQSPVTSHPASAGPADPLLVEDLVAANRILAQEGVLDGWG
                     HVSVRHDRDPSRFLLSRSLAPELVSAEDIIEFDLDNNPVDARGRDLYTERFIHGEIYK
                     ARPDVKAICHTHAP"
     gene            complement(391..1479)
                     /locus_tag="AC16CX_09565"
     CDS             complement(391..1479)
                     /locus_tag="AC16CX_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3012797.1"
                     /translation="MKNNNLKLDIFPHIFPQSFFERMKGIAAGNPALAASIKRWLNIP
                     VLWDLDARLRMMREFKDYKQVLTLSLPAIEFLAGPEESPVLARLANDGMAGIVDRYPD
                     RFPAFVASLPMNNVPAALEEMDRAILKLGAKGIQVFTNVNGRPLDEPEFYPIFERCVR
                     KYDLPIWLHPVRTAKFADYAAESKSKYEIWWLFGWPYETSACMARIVFSGMLQKLPDM
                     KIITHHLGAMAPFFDERIGLGMDQMGTRTVDEDYGELLKRLGKRPLEYFKMFYGDTSV
                     NGSAPAIRCGLDFFGPDRVLFGTDCPFDPEGGPTFIRESIRAINSLKLAGPVRRKVYY
                     GNAIKMLRLDLPAAAPPARKAKAKARKK"
     gene            1715..2086
                     /locus_tag="AC16CX_09570"
     CDS             1715..2086
                     /locus_tag="AC16CX_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012798.1"
                     /translation="MRLTKTMLALAVVLAGIMASGSALAHHGGRFHHRSSVHFGVFLG
                     APAFWYYPPPYYYYPPAYYYPPVIAAPASPPAYIERGDANAPQEQPQGYWYYCPEAKA
                     YYPYVKQCAGGWQRVPAQPPS"
     gene            2224..2643
                     /locus_tag="AC16CX_09575"
     CDS             2224..2643
                     /locus_tag="AC16CX_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR3012799.1"
                     /translation="MSLPGTGKSFDQFRADDYECRQYASVQAGGTTPNQASADSGVKS
                     AAVGTAIGAAAGAAMDGSHGAATGAGVGLLVGALAGTGAANASAHTAQQRYDIGYTQC
                     MYAKGHKVPVSGRFVSANERPAPRYYTPPPPPPPPAR"
     gene            complement(2769..>3705)
                     /locus_tag="AC16CX_09580"
     CDS             complement(2769..>3705)
                     /locus_tag="AC16CX_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012800.1"
                     /translation="IGVDATDLGAWRAFGTDVLAMQIVERGADALALRIDERCQRVLV
                     QRAERDGPAFFGFETTGERALKETVGKLEAAGVSMTRASEKELMLRRVDDMAWCRDPA
                     GNRIEFYHGQAAGNGPFTPPRPHGGFRTGRLGMGHMVLYAEDIDAMSAFYRELLGLRL
                     SDYMVKPYRLMFLRANPRHHSVGLLQSSRSGVHHVMFEVLALDDVGRAYDFALEGWRI
                     GQTLGRHANDWMLSFYAFCPAGFMMEYGWGGRTVDEATWTPREVTHGGSMWGHDRLWL
                     DEDRRRAANEVRRATIMAGLGAPLQVTRGQYNEVE"
BASE COUNT          629 a         1230 c         1197 g          649 t
ORIGIN      
        1 ggcgcgtgcg tatggcagat cgccttgacg tcgggccgcg ccttgtagat ctcgccgtga
       61 atgaagcgct cggtgtaaag atcgcgcccc ctggcgtcca ccggattgtt gtcgaggtcg
      121 aactcgatga tgtcctcggc gctcaccagc tccggcgcga gcgaccgcga cagcagaaaa
      181 cggctggggt cgcggtcgtg ccgcacgctg acgtgccccc agccgtcgag cacgccctcc
      241 tgcgcgagga tgcggttcgc cgcgaccagg tcctcgacca gcagcggatc cgccggcccg
      301 gcggaagccg ggtgacttgt gactggtgac tggtgactgg tggacatgtg gccagtttac
      361 gccctgaatc gggtcagtcc ctgaacgatg tcacttcttg cgcgctttgg ccttcgcctt
      421 gcgcgcaggc ggggcggccg cgggcaggtc cagccgcagc attttgattg cattgccata
      481 gtacaccttg cggcgcaccg gcccggcgag cttgagcgaa ttgatcgccc ggatgctctc
      541 gcggatgaag gtgggccccc cttccgggtc gaaggggcag tcggtgccga acaacacccg
      601 gtcggggccg aagaaatcca gtccgcagcg aatcgcgggc gcggagccgt tgaccgaggt
      661 atccccgtag aacatcttga aatattccag cggccgcttg cccagtcgct tcagcaactc
      721 gccgtagtct tcgtccacgg tgcgcgtgcc catctggtcc atgccgagac cgatgcgctc
      781 gtcgaagaac ggcgccatcg cgccaaggtg gtgggtgatg atcttcatgt cgggcagttt
      841 ttgcagcatg cccgagaaca cgatgcgcgc catgcaggcg ctggtctcat agggccagcc
      901 gaacagccac cagatctcgt acttcgactt ggattccgca gcgtagtcgg caaacttcgc
      961 ggtgcgcacc gggtgcagcc agatcggcag gtcgtatttc ctgacgcagc gctcgaagat
     1021 cgggtagaac tccggctcgt cgagcggacg gccgttgacg ttggtgaaga cctggatgcc
     1081 cttggcgccg agcttgagta tcgcgcggtc catctcctcc agcgccgccg gcacgttgtt
     1141 catcggcagg gaggcgacaa aggccgggaa ccgatccggg tatcggtcaa cgatccccgc
     1201 catcccgtcg ttggcgagcc gggcgagcac cggcgattcc tccggcccgg cgaggaactc
     1261 gattgccgga agcgacagcg tcagcacctg cttgtagtcc ttgaactcgc gcatcatccg
     1321 cagccgcgcg tcgagatccc acagcaccgg aatgttgagc cagcgcttga tcgatgccgc
     1381 aagcgcggga ttgccggcgg cgatgccttt catgcgctcg aagaaggact gcgggaaaat
     1441 gtgcggaaag atatccagct tcaggttgtt gttcttcatg ggcaattccc ggtcaggatc
     1501 gaggatggaa aaagccccca ccttagacga tctcccgcgc cgccgcaaat tgcatggggc
     1561 tccgtgtcgc tggcccccga caggccgtgc agggacggaa tcaggacggt tccagggcgc
     1621 gcggctgcgt aatactttgt aacacttttt tacccgaacg gctgccaggc tctgatagtc
     1681 ttactgacag gtcttccgca aggaggatgc aatgatgagg ctcacgaaaa cgatgctggc
     1741 gctggccgtg gttctggcgg gaatcatggc gagtggatcg gcgctggctc atcatggagg
     1801 gcgtttccac caccgctcta gcgtgcactt cggcgttttc ctcggcgctc ccgccttctg
     1861 gtattaccca cccccctatt actactaccc gcctgcctac tattacccgc cggtgatagc
     1921 ggcgccggcc tcaccaccgg cgtatatcga gcggggtgat gccaatgccc cgcaggagca
     1981 gccccagggc tactggtact actgccccga agccaaggcc tattatccct acgtcaagca
     2041 atgcgcggga ggctggcagc gagtgccggc acagccgccc agctgatctt agggatgagg
     2101 gcggagggat gaaggatgag gattaacttc gatttatctt gtctgtcttg gcgccttggc
     2161 ggttcgatct tgtcgctgtt cgcgctcgcc gcgtgcgtgt cggtgccgag cggccccagc
     2221 atcatgtccc tgccgggcac cggcaagagc ttcgaccagt ttcgcgcgga cgactacgag
     2281 tgccggcagt acgccagcgt gcaggcgggc gggaccacgc ccaatcaggc gtccgccgat
     2341 agcggcgtaa agagcgccgc cgtcggcacg gcgataggcg ccgcggcggg cgccgccatg
     2401 gacggcagcc acggcgcggc caccggcgcc ggggtcggcc tgctggtagg cgccctcgcg
     2461 ggaacgggtg ccgcgaatgc ttctgcccac accgcgcagc agcgctacga catcggctac
     2521 acgcagtgca tgtacgccaa gggccacaag gtcccggtat ccgggcgctt cgtgtccgcg
     2581 aacgagcggc ccgcgcctcg ttactacacg ccgcctcccc cgccgccacc gcctgcgaga
     2641 tagaaacggc gtggatcgtg gttcgtgggt cgtgatacga cctcaataaa aaagcccgcg
     2701 ccaccgcggg ctttttaatg tggatgcgaa tccgttcatg aaaaaggccc ggacatgccg
     2761 ggcctttttc attcgacttc gttgtactgt ccgcgggtga cctgcagcgg cgcgcccagg
     2821 cccgccatga tggtcgcgcg ccgcacctca ttggcggcgc ggcggcggtc ttcatccagc
     2881 catagccggt cgtggcccca catgctcccg ccgtgcgtaa cctcgcgcgg cgtccaggtc
     2941 gcctcgtcga ccgtgcgccc gccccagccg tactccatca tgaagccagc cgggcagaac
     3001 gcgtagaacg aaagcatcca gtcgttggca tggcggccga gcgtctggcc gatgcgccag
     3061 ccctccagcg cgaagtcgta ggcgcggccg acgtcgtcga gcgcgagcac ctcgaacatg
     3121 acgtggtgca cgccgctcct ggacgactgc agcagcccga cgctgtggtg gcgcgggttg
     3181 gcgcgcagga acatcaggcg gtacggcttg accatgtagt cggagagccg caagccgagc
     3241 agctcgcggt agaacgcgct catggcgtcg atgtcctcgg catacaacac catgtgtccc
     3301 atgcccagcc gtccggtgcg aaagcccccg tgcgggcgcg gcggcgtgaa cggcccgttg
     3361 cccgccgcct ggccgtgata gaactcgatg cggttgcccg ccgggtcacg gcaccaggcc
     3421 atgtcgtcca cccggcgcag catcagttcc ttctcgctgg cacgcgtcat cgagacgcct
     3481 gccgcctcga gcttgccgac ggtctccttc aacgcccgct cgccggtggt ctcgaatcca
     3541 aaaaacgccg gcccgtcgcg ctcagcgcgc tgcaccagca cgcgctggca gcgctcatcg
     3601 atgcgcagcg cgagcgcgtc cgcgccgcgc tcgacgatct gcatcgccag cacgtcggtc
     3661 ccgaacgcgc gccacgcgcc gagatcggtc gcatcaacgc cgatg
//
LOCUS       DBNGWZ010000335        11871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_793106, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000335 DBNGWZ010000000
VERSION     DBNGWZ010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_793106"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1442
                     /locus_tag="AC16CX_09585"
     CDS             120..1442
                     /locus_tag="AC16CX_09585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3012801.1"
                     /translation="MDQPALAVSPQPLLVSWNITGLCNLACSHCYLDAARRRRGSRNE
                     LDTVAALGIVSQIAALAPGAMLVFSGGEPLLRRDLGMLVRVAAARGLTPVVGTNGTLL
                     SERRANRLKEAGAAGIGISLDSAETAFHDRLRGVSGAWRAARRGAVAARRAGLALLLQ
                     ATLFEENRNDLAALADMAESLGAVALNFFFLVCTGRGASQTDLTPAAYEETLARIIEL
                     QYAHPHLIIRARCAPHLRRRLGLHAGEGDHHYAAWSSACLAGRGYFRITPRGGVTPCP
                     YIPTIVGELATTPLREIWERHPLLLRLRTEHATGKCGDCNFRYSCGGCRARALARHED
                     LFAEDPNCLYVKPPEARPESPPAASVRREVDWEPEACALLERIPAFVRATVKARVEER
                     AAREGRECITLDFMCASRPPFESVGCTFGRHLQTESVIHSDLTKGVDR"
     gene            1492..2895
                     /gene="hemG"
                     /locus_tag="AC16CX_09590"
     CDS             1492..2895
                     /gene="hemG"
                     /locus_tag="AC16CX_09590"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR3012802.1"
                     /translation="MARVIVAGAGISGLTAAYLLHSRGHEVSVFDRDDTPGGRMRSER
                     LGGFLMEHGANSLVGPAVAAENLITALDLGGEKVMRNPAARHRYLVRDARARALPVEP
                     CRFFLSDFLSFAGRLRLLMEPFAAVHRDDETVAAFTRRRLGQEMLDYVMDPLVAGLYA
                     GDPQRLSVSALFPQFKRLECRYGSIILGAIGSRLRRGGEPAACGPGKRTLFSFRQGLG
                     MLPRAIAKQLGGRIFLGHRVESLRRGAGGCFRVSVRRGASVRWIMADSVVIALPAHAV
                     ASVLSGFDGPTAETLAEISHPPLAVVFLGYRASTIAHPLDGLGVLMPSVEKRGVLGML
                     FSSSLFPGRAPPGHVAITAFVGGSRQPWLATLEPDELTPLVHREVRELLGGSEAPVVT
                     RTRCWRHGLPQPGVDHARRLSRIAAAESEHTGLFLTGNYFSGVSTAACIEQAVNTARR
                     VERYLAAARSGGARKAA"
     gene            3073..3684
                     /locus_tag="AC16CX_09595"
     CDS             3073..3684
                     /locus_tag="AC16CX_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3012803.1"
                     /translation="MRELLYKSGTAIGVALLPAAALAVQEEVLTLTVSILPMYAFILF
                     VIIVIVGAYFMRSRDKRRTPLDMIFEEGEAIYSVGPDTLVTECVRTMTAEKIGALLVM
                     DGERLIGIFTERDALNKVLAAGLDPGNTKVFDVMTKDPYCIPPTTTVGDAMELVTKRR
                     FRHLPIVKNGKVVAVVSSGDLIHWLVKDQVREVQELVDFAARS"
     gene            complement(3778..4056)
                     /locus_tag="AC16CX_09600"
     CDS             complement(3778..4056)
                     /locus_tag="AC16CX_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012804.1"
                     /translation="MRYHIEKQETGISIRIDDVAGQEQAVLETIRQCRRQSVWACPSG
                     ECQNIGSMKERAEGGSVFLTLTPTPGAEITLSGIDECLRYVLHQAVKV"
     gene            complement(4173..4814)
                     /gene="nth"
                     /locus_tag="AC16CX_09605"
     CDS             complement(4173..4814)
                     /gene="nth"
                     /locus_tag="AC16CX_09605"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR3012805.1"
                     /translation="MNPQQRHEIFSRLRSANPAPRSELVYASPFELLVSVVLSAQATD
                     KSVNLATTDLFRTANTPAALLKLGRKGLERHIRRIGLYRTKAKNLLATCKLLIEQHGG
                     EVPRTRAELETLPGVGRKTANVILNTAFGEPTIAVDTHIFRVANRTGIAPGKDVRAVE
                     EKLLRFVPDEFRLDAHHWLILHGRYVCKARKPDCPACLIRDLCEFKGKTRVEG"
     gene            4935..5483
                     /locus_tag="AC16CX_09610"
     CDS             4935..5483
                     /locus_tag="AC16CX_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012806.1"
                     /translation="MNKPQIIGRIVLSMIRVLPSLRLILAAILSAAASAAAQQVTLER
                     DSPLYAEPRPEAAPVAQLKQGATGEVVGKQGGWLNLKTSAGTGWLFSFNVRFPAQKAE
                     SGESGAGSALGRVFGPHRPTTVVSAIGIRGLEEEDLKQASFDAGQMKLLDAYAASKQA
                     AEEGARAAGLAPAKVEYLDAKK"
     gene            5696..6631
                     /locus_tag="AC16CX_09615"
     CDS             5696..6631
                     /locus_tag="AC16CX_09615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3012807.1"
                     /translation="MVVIATLSGHAVAASIDLGRIFGAGKDLATAASGLDEKDEVAVG
                     REVAGRMLGAAPLVADPGLQSYVNRIGRWVATQTERPDLPWHFGVLDSPAINAFAAPG
                     GYVLITRGLYEILDNEAQLAGVLGHEIGHIVRRHHITVMQKSAAISAGAQLAQARDRS
                     VLVSNLIGTGAEIFARGLDKSAEFEADQIGIVLAARAGYNPYGLIEVLHKLSARGAED
                     ASLALLFKTHPHPNERLSQLGETLAPRAAGLPSGSEPAIRQVSAEAPPAPAKPVPAEG
                     ARALQQEEPGPAVRPGGRGGVGIDPGGILRGILGR"
     gene            complement(6743..7096)
                     /locus_tag="AC16CX_09620"
                     /pseudo
     CDS             complement(6743..7096)
                     /locus_tag="AC16CX_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
     gene            complement(7199..8224)
                     /locus_tag="AC16CX_09625"
     CDS             complement(7199..8224)
                     /locus_tag="AC16CX_09625"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006476114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR3012808.1"
                     /translation="MIYSLLRPLLFALEAETAHHFTLEALRRLYDLGLVPAPASAPST
                     CARSVMGLDFPNPVGLAAGLDKNGDCIDALARLGFGFLEIGTVTPRPQPGNPRPRVFR
                     LPAARAIINRLGFNSDGVDRLVENVKRARFHGVLGINLGKNADTPLERAAEDYVACLR
                     KVYSLASYVTINISSPNTRDLRQLQQASELDQLLGALKSEQRRLADVHGKHVPLAVKI
                     APDLDDAQIAAVAGLLQRHGVEAVIATNTTTTRDGVSGLAHADEAGGLSGAPLTMRST
                     KVVRELARALRGDIPIIAVGGIMSGADARDKIAAGASLVQLYTGLVYRGPELVRECVN
                     ALCAKGS"
     gene            8427..9485
                     /locus_tag="AC16CX_09630"
     CDS             8427..9485
                     /locus_tag="AC16CX_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3012809.1"
                     /translation="MIPEKRIEQIIQVAAITVLVIGCLMVLRPFLAAILSAAILCFST
                     WPVYEWIERKVGKRGWLAALVMTLLLILVLVLPLVAVALTYADEVPHLLERVRELLAG
                     GLPDPPSWVASIPLVGEPLDSGWRELAGSRAKLAEALQRLLQPAREVLVQTGILIGEG
                     ALQFILIAFIGYFFYSDGAALFKALRSALNRVAGDLTERLLQIVGGTINGVIYGIVGT
                     GIAQGLAALIGFLIAGVPGALMLGFFTVFLSIIPAGPALLWVGATVWLFYQDQPGWAV
                     FMALWGFFVISGIDNIVKPLLISRGASLPFALVLLGVLGGVLAFGFVGIFLGPVLLAL
                     GFNLARRWTQARAGLPSG"
     gene            complement(9501..10421)
                     /locus_tag="AC16CX_09635"
     CDS             complement(9501..10421)
                     /locus_tag="AC16CX_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3012810.1"
                     /translation="MRLALKIDFDTYRGTREGVPRLLRALERANARATFLFSVGPDHT
                     GRALRRVFKRGFLGKVARTSVIRHYGLRTLLYGTVLPGPDIGAACREEMRAVQAAGFE
                     TGLHAWDHVEWQDYVAVRGPAWTETEWRRGVDRYAQIFGELPRVHGAAGWQMNADAFR
                     LEEQGPIDYASDTRGRHPFIPVIEGSPMTRPQLPTTLPTLDELIGTQGCDASNVDQLL
                     LCHTAQDPASQAHVFTLHAELEGLQLLPVLERLIGGWRSQGYELVAMRQIFDRLDVAN
                     LPFHTVEPGTVPGRAGTLMVQGGRVPAGRP"
     gene            complement(10449..11492)
                     /locus_tag="AC16CX_09640"
     CDS             complement(10449..11492)
                     /locus_tag="AC16CX_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HIR3012811.1"
                     /translation="MKKILILGVNGFIGHHLSKRVIETTPWEIYGMDMNTDRIADLMG
                     EKRFHFFEGDITINKEWIGYHIRKCDTVLPLVAIATPATYVAEPLRVFELDFEANLPI
                     VRACVEYHKRLVFPSTSEVYGMCRDGQFDAETSELVLGPINKQRWIYSCAKQLMDRVI
                     WAYGVERRLDFTLFRPFNWIGPGLDNLHADKEGSSRVVTQFLGQIVRGDPIKLVDGGR
                     QRRSFTYVDDGIDALMKIIENPGRVASGKIYNIGNPKNNLSIRELAQLMVEIALTYPE
                     YRERARKVRIGTTTAAKYYGTGYQDMQNRVPKIDVTRRDLDWRPRIGMREALKRIYDA
                     YRGHIPDARRLVT"
     gene            complement(11501..>11871)
                     /locus_tag="AC16CX_09645"
     CDS             complement(11501..>11871)
                     /locus_tag="AC16CX_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012812.1"
                     /translation="QDQASATYFGARRPEDGRIDWTLGAHAIHNLVRGVAPPYPGAFT
                     QLAGRNLRVLRTRVERECGTPAAPSIHIDRNGCFACCADGGVLRLLDLEMDGLGVQPQ
                     ALEALLGRRAPFPLQQHPRN"
BASE COUNT         1937 a         3771 c         3971 g         2192 t
ORIGIN      
        1 caggaaggga cggcgaaagc ggggaaatgc ccgttcgcgc atcgcgccaa cgagggcagg
       61 cgcaagccgc gccggacgga gccgatcgcg gatcaaggac ggaaatgaca ctgccagcca
      121 tggatcagcc cgctctcgcg gtgtcaccgc aaccgctgct cgtttcctgg aacataacgg
      181 gtttgtgcaa tcttgcgtgc agccactgct atctcgatgc ggcgcggcgc aggcgcggga
      241 gccgcaacga gctggatacc gtcgctgctc ttggcatcgt gagtcagatc gccgcgctcg
      301 cacccggcgc aatgctggtt ttcagcggcg gcgagccctt gctgcgccgc gacctgggga
      361 tgctggtgcg ggtggccgcc gcgcgcggcc ttacaccggt cgtcggcacc aacggcacgt
      421 tgctgagcga acggcgggcc aaccggctga aagaggcggg tgccgccggg atagggatca
      481 gcctcgattc cgcggaaacc gcatttcatg accggctgcg cggagtgtcc ggcgcctggc
      541 gagccgcgcg gcgtggagcc gtcgccgcgc ggcgcgcggg cctggcgctg ctgttgcagg
      601 ccacgctgtt cgaggagaac cgcaacgatc tcgcggcgct cgccgatatg gccgaatcgc
      661 tgggggcggt ggcgctgaat tttttcttcc tcgtatgcac cgggcgcggc gcgtcgcaaa
      721 cggatttgac ccccgccgcg tacgaggaga cgctggcgcg catcatcgag ctgcaatacg
      781 cgcaccccca cctcattatt cgcgcgcgtt gcgcgccgca tttgcgccgg cggctcggcc
      841 tgcacgctgg cgaaggcgat catcactacg ccgcttggtc cagcgcgtgc ctcgcggggc
      901 gcggctactt ccgcatcacg ccgcgcggcg gggttacgcc atgtccctac ataccgacca
      961 tcgtcggcga actcgcgaca actccgttac gagagatctg ggagcggcat ccgctgcttc
     1021 tgcgcctgcg caccgagcat gccacgggca agtgcggcga ctgcaacttc cgctacagct
     1081 gcggcggctg ccgcgcgcgg gcgctcgcgc ggcacgaaga cttgtttgcg gaggatccga
     1141 actgcctgta cgtgaagccg cccgaggcgc ggccggagtc accgcctgcc gcttcggtac
     1201 gccgggaggt cgactgggag ccggaagcgt gcgcgctcct agaacggatt ccggcgttcg
     1261 tccgtgccac ggtgaaggcg cgcgtcgaag agcgcgccgc gcgcgaggga cgcgagtgta
     1321 tcacgctgga tttcatgtgc gcctcgcgcc cgccgttcga gtctgtcggc tgtactttcg
     1381 ggcggcactt acagacagaa tcggtgatcc atagcgacct taccaaagga gttgaccggt
     1441 gatcacccga tgctcggaat tcgcgatgtt cgacccgggt agcgtaacgc gatggcgaga
     1501 gtgatcgtcg ccggagccgg catctcgggc ctgaccgccg cttacctgct gcacagcaga
     1561 gggcatgagg tctcagtttt cgatcgggat gatacgccgg gcgggcgcat gcgttcggag
     1621 cggctcggcg gctttcttat ggaacacggc gcgaacagcc tggtcggtcc cgcagtggct
     1681 gccgagaacc tgatcaccgc gctcgacctc ggtggcgaaa aagtgatgcg caacccggcg
     1741 gcgcgccacc gctatctggt acgtgacgct cgtgcgcgtg cgttaccggt ggagccgtgc
     1801 cggttttttc tgtccgattt tttgtcattt gccggccgcc tgcggctgct catggagccg
     1861 ttcgcggcgg tgcaccgcga cgacgaaacg gtcgcggctt tcacgcgcag gcggctgggg
     1921 caggaaatgc tggactacgt gatggacccg ctggtagccg ggctatatgc cggggaccca
     1981 cagcgattga gcgtatctgc gttgtttccg cagttcaaac gcctcgaatg ccgctatggc
     2041 tcgatcatcc tcggtgcgat cggctcgcgc ttgcggcgcg gtggtgagcc ggccgcttgc
     2101 gggccgggaa agcgcacgtt gttttcgttc cgccagggcc tggggatgct gccgcgcgcg
     2161 atcgcgaaac agttgggagg acgcatcttt ctgggacacc gtgtcgagtc gctgcggcgc
     2221 ggtgccggcg gctgttttcg tgtgtcggtc cgccgtggtg catccgtacg gtggatcatg
     2281 gcggacagcg tggtcatcgc gctgccggcc cacgccgtgg caagcgtact cagcgggttc
     2341 gacggaccga cggcagaaac attggcggaa atcagccatc ccccgcttgc tgtggtcttt
     2401 ctgggttacc gggcgagcac aatcgcgcat ccgcttgacg gtctgggcgt gctgatgccc
     2461 tcggtcgaaa aacgcggcgt cttgggcatg ttgttttcgt cgtcgctttt tccgggtcgc
     2521 gcaccgccgg ggcacgtcgc cattacggcc ttcgtcggcg gctcgcgcca gccgtggctc
     2581 gcgacgctgg agccggatga gcttacgccg ctggtccacc gtgaagtgcg ggagctcctc
     2641 ggcggaagcg aggcaccggt ggtgacccgt acccgttgct ggcggcacgg gctgccccag
     2701 ccgggtgtgg atcacgcgcg gcgactcagc aggatcgcgg cggccgagag cgagcatacc
     2761 ggcctgttcc tgaccggcaa ttatttttcc ggcgtctcca cggcggcctg catcgaacag
     2821 gcagtgaata cggcccgtcg cgttgagcgt tatcttgccg ccgcgcgatc agggggcgcg
     2881 agaaaggcag cgtaaccaat ttcacgttgg gcatctggac tgttgcaacg cgcttgacgc
     2941 cgctcgtagc cagatgcaat gtataaacgt cgaaaacggc gcaggttgag gacgccggcc
     3001 cctgcccgtg acattgcggc aataaacgag agactggggc gtagtcggga ttgcaatcga
     3061 ggagccaaag cgatgagaga gcttctttac aaatcgggca ccgctatcgg tgttgcactg
     3121 cttcccgccg cagcgctcgc tgtgcaggag gaggtactga cgctgaccgt cagcatattg
     3181 cccatgtacg cgttcattct gttcgtgatc atcgtcatcg tcggcgcgta cttcatgaga
     3241 tcacgggaca agcgcaggac tccattggac atgatattcg aggagggtga agcgatatac
     3301 tccgttggac ccgataccct ggtcaccgag tgcgtgcgca cgatgaccgc cgagaaaatc
     3361 ggtgccttgc tcgttatgga tggcgaaagg ttgatcggca tcttcacgga gcgggatgcg
     3421 ctgaacaaag tgctggctgc cggccttgat cccggcaaca cgaaagtctt cgatgtgatg
     3481 acgaaggacc catactgcat cccgccgacg acgactgtcg gcgacgcgat ggagttggtc
     3541 accaagcggc ggttccggca cctgccgatt gtgaaaaacg gcaaggtggt cgccgtcgta
     3601 tcgagcggcg atctaatcca ttggctggtc aaggaccagg tgagagaggt tcaagagctt
     3661 gtcgattttg ccgcccggtc ttgaccgccg aatccgggcc ggacaacgtg accaagagcg
     3721 ggccctacct ggctagattc ggcgtcaggc cgcagcacgg tcatcttgga gcgcagccta
     3781 taccttgacg gcttggtgca gcacgtagcg taggcactcg tcgatgccgg agagggtgat
     3841 ctctgcgccg ggcgtagggg tgagggtcag gaaaacacta ccgccctctg cgcgttcctt
     3901 catcgatccg atgttctggc actcccccga agggcaagcc caaacgcttt gcctacgaca
     3961 ttgacggatc gtctcgagga cagcctgctc ctgccccgcc acgtcatcga tgcggatgct
     4021 gatcccggtc tcctgtttct cgatgtggta tctcataaag cccccgtctg tctggccgct
     4081 ccagccgaag aaggttcgaa agactataca cgggttcgga cgctgcttgt cagatcaagt
     4141 ctgaactact atagatcagg gatgtttcac gctcaaccct caaccctcgt ctttcctttg
     4201 aattcgcaca gatcccgaat caaacacgcc gggcaatcag gtttcctcgc cttgcagacg
     4261 tagcgaccgt gcaggatcag ccagtggtgg gcgtccagcc ggaattcatc gggcacgaat
     4321 ctgagcagct tctcctcgac cgcccgcacg tccttgcccg gcgcgatgcc ggtgcggttc
     4381 gccacgcgga agatgtgggt gtccaccgcg atggtcggct cgccgaacgc ggtgttgagg
     4441 atgacgttgg cggtcttgcg gccgacgccc ggcagcgtct caagttccgc gcgcgttcgc
     4501 ggcacctcgc cgccgtgctg ctcgatgagc agcttgcagg tggcgagaag gttctttgcc
     4561 ttggtgcggt agaggccgat gcggcggata tggcgctcca ggcccttgcg gccaagcttg
     4621 agcagggcgg cgggcgtgtt cgccgtcctg aacaggtcgg tcgtggcgag gttcacgctc
     4681 ttgtcggtgg cctgcgccga cagcacgacg gagaccagca gctcgaacgg cgaggcatag
     4741 acgagctcgc tccgcggcgc gggattggcg gagcgcagcc gggagaagat ctcgtggcgt
     4801 tgctgcgggt tcatgagggc tgccgaaacg tggcggaatt cacgccgtgg catggcgtgg
     4861 cgatccgccg ctcattataa tcagcaagga gtttgccgga atgcctccgg caaactcctg
     4921 aatctggccc ctgaatgaat aagccgcaaa ttatcggcag gatcgtgctt tccatgattc
     4981 gcgtgctgcc gtcgcttcgc ctgattctgg cagcgatact ttcagccgcc gcttcagcgg
     5041 cggctcagca ggtcacgctg gagcgcgact cgcccctcta cgcggaaccc cgccctgaag
     5101 cggccccggt ggcgcagctc aagcaaggcg cgaccggcga ggtcgtaggc aagcagggcg
     5161 gctggctcaa tctcaagaca tcggccggaa ccgggtggct cttttcgttc aacgtgcgct
     5221 tcccggccca gaaggcggag agcggggaat cgggcgccgg ctcggcgctg gggcgggtgt
     5281 tcggcccgca ccgccctacc accgtcgtca gcgccatcgg catacgcggc ctcgaggaag
     5341 aggatctcaa gcaggcaagc ttcgatgccg gacagatgaa gctgctcgat gcctatgccg
     5401 cctcgaaaca ggcggccgag gagggcgcgc gcgcggcggg actcgcgccg gcgaaggtcg
     5461 aatacctgga cgcgaagaaa tgaggaattt gctcgccttt ggccgacaaa ttcctgaatg
     5521 caaagccgcc cgaggacata ggatgaacca agaccagatg cttctttcgg accgctgtcc
     5581 gtggcggcaa cggggtcgaa tccgtgcggc tctcgtccat ttccagagat tcattccgga
     5641 tggttttgcc tcaggaattc gtcgggtctt ggcccgacga attccttggg ttgccgtggt
     5701 cgtgatcgcc accctatccg gtcatgcggt cgcggcctcg atcgacctcg gccgcatttt
     5761 cggtgcaggc aaggacctgg cgaccgccgc cagcggcctg gacgagaagg acgaggtcgc
     5821 ggtgggccgc gaagtagcgg ggcgcatgct cggcgcggcg cccctggtgg ccgaccccgg
     5881 gctgcaatcg tacgtcaacc gcatcgggcg ttgggtcgcg acgcagaccg agcgccccga
     5941 cctgccgtgg catttcggcg tgctagacag cccggcgatc aacgccttcg cggcgcccgg
     6001 cggttacgta ttgatcacgc gcggtctcta cgagatcctg gacaacgagg cgcagctcgc
     6061 gggcgtgctc gggcacgaga tcggccacat cgtgcggcgt catcacatca cggtgatgca
     6121 gaaaagcgcc gcgatctcgg cgggggctca actcgcgcag gcgcgggacc gctcggtcct
     6181 cgtcagcaat ttgatcggca ccggcgccga gatttttgcg cgcgggctcg acaagagcgc
     6241 cgaattcgag gccgaccaga tcggcatcgt gctcgccgcg cgcgccggct acaacccgta
     6301 tggcctgatc gaagtgctgc acaagctctc ggcgcgcggt gcagaggacg catcgcttgc
     6361 gctgctgttc aagacccacc cccatcccaa cgagcgcctg tcgcagcttg gcgaaacgct
     6421 tgcgccgcgc gcggcaggac tgccgtccgg aagcgagccc gcgatccggc aggtcagcgc
     6481 cgaggcgccg cccgccccgg cgaagccggt tcccgcggaa ggcgcgcgcg ccttgcagca
     6541 ggaagagccc ggccccgcgg tgcggccggg cggcaggggc ggcgtcggca tcgatccggg
     6601 cggcatcctg cgcggtattc ttgggcgcta aaggcggtga ttaagtgact aggtgattaa
     6661 gtgattaagc aaccaagtaa gcaaatcgct aggccactta gtcactcaat cacttagtta
     6721 ctcagtcact cgatcggcgg agccgatcgc gagcgcgctg cacggcgcgc tcgatggtcg
     6781 cgcgcttctt gcgcccggct gcccgcctcc gctcggcctc gatccgggtc ttgcgctcga
     6841 gccgagccgt tcgcgcatcg tggcgccgcc ggtactggct ggcgcgcctc aggcgctctt
     6901 cgtgcgccca ggtttcgccg gtttcgacca tcgcgatgca gtccacggga cacggcggca
     6961 ggcagcgctt acagccggtg cactcggccg cgatcacggt gtgcatgagc ttcggcgcgc
     7021 cgacgatggc gtccaccgga cacgcctcga tgcacaacgt gcagccgata caggtctgct
     7081 cgtcgatcac ggcaactgcg ggggttttaa ctgagtctgg agccataaga ccgtgattcg
     7141 ttaagagtgt cgggtgtcga gtgtaaaacc tctcgtccct cggaacttcg tcaccgcgtc
     7201 agcttccctt tgcgcatagc gcgttgacgc actcgcgcac cagttccggc ccgcgataga
     7261 cgagccccgt gtacagttgc acgaggctcg cccctgcggc gattttatcc cgcgcatcgg
     7321 cgccgctcat gatgccgccg accgcgatga tcggaatgtc gcccctcaaa gcccgcgcga
     7381 gctcgcgcac caccttcgtc gagcgcatcg tgagcggcgc accgctcaat ccgcccgctt
     7441 cgtcggcgtg cgccaggccg gaaacgccgt cgcgggtcgt cgtggtattg gtggcgatca
     7501 cggcctccac gccgtgccgc tgcagcaggc cggcgaccgc cgcgatctgc gcgtcgtcga
     7561 gatcgggcgc gatcttcacc gcgagcggaa cgtgtttccc gtggacgtcg gcgagccggc
     7621 gctgttcgga tttcaacgcg ccgagcagtt gatcgagctc cgacgcctgc tgcagctgac
     7681 gcaggtcccg ggtgttgggc gaagagatat tgattgtcac gtaactcgcg agggaataga
     7741 ccttgcgcag gcacgcgacg taatcttccg cggcccgctc gagcggcgta tcagcgttct
     7801 tgccgaggtt gatgccgagc acgccatgga agcgcgcgcg cttgacgttt tccaccaggc
     7861 ggtccacgcc gtcgctgttg aatccgaggc ggttgatgat cgcgcgcgcg gcgggcagtc
     7921 ggaacacgcg cggccgcgga ttgcccggtt gcgggcgcgg cgtcaccgtg ccgatctcga
     7981 ggaagccgaa gccgagccgc gcgagggcat cgatgcagtc gccgttcttg tcgagaccgg
     8041 ccgccagtcc caccgggttc ggaaaatcga ggcccatgac gctgcgcgcg caggttgacg
     8101 gcgcggaggc gggggcagga acgagcccca agtcatataa ccttcgcagc gcctcgagcg
     8161 tgaagtgatg cgcggtctcg gcttcgagcg cgaacagcag cgggcgcagc agcgagtaaa
     8221 tcatccgccc tattttacag gcgatcttgt tcttgccggc ctgtcagacc atcaaggccg
     8281 gagacttgcg cgggcggatg atttacttat ttggaattta catttaaggc gggcgacgcg
     8341 atgcgcgagt acacgcgatg tcgatgccga gcccgccttc ttatttgacc gtgcgcgcac
     8401 cgcgtgatag aacgcgcggg gaagccatga tcccggaaaa gcgcatcgag cagatcatcc
     8461 aggtcgccgc gatcacggtg ctcgtgatcg gctgcctgat ggtgctcagg cctttcctcg
     8521 cggcgatcct ctccgccgcg atcctctgct tttcgacctg gccggtctac gagtggatcg
     8581 agcgcaaggt cgggaaacgg ggctggctcg ccgcgctggt gatgaccctg ctgctcatcc
     8641 tggtgctggt gctgccgctg gtggcggtcg ccctcaccta tgcggacgag gtcccgcacc
     8701 tgcttgagcg cgtgcgcgag ctgctcgccg ggggcctgcc cgatccgccg tcctgggtcg
     8761 cctcgatccc gctcgtgggc gagccgctcg acagcggctg gcgcgagctg gcgggcagcc
     8821 gggcaaaact cgcggaggcg ctgcaacgcc tgttgcagcc ggctcgcgag gtgctggtgc
     8881 agaccggcat cctgatcgga gagggcgcgc tgcaattcat cctcatcgct ttcatcggtt
     8941 acttcttcta tagcgacggt gcggcgctgt tcaaggcgct gcgcagcgcc ttgaaccgcg
     9001 tggccggcga tctcacggag cgactgctcc agatcgtcgg cggtaccatc aacggcgtga
     9061 tctacggcat cgtcggcacc ggcatcgcac aggggctcgc tgccctgatc ggcttcctga
     9121 tcgcgggggt gccgggcgcg ctgatgctgg gatttttcac ggtttttctt tccatcattc
     9181 ccgcgggccc ggcgctgctc tgggtcggcg caacggtctg gctgttctat caggaccagc
     9241 cgggatgggc cgtgttcatg gcgctgtggg ggtttttcgt gatcagcgga atcgacaata
     9301 tcgtcaagcc gctgctcatc agccgcggcg cgagtctgcc gttcgcgctg gtgctgctcg
     9361 gcgtgctcgg cggcgtactc gcgtttggct tcgtcgggat tttcctgggt cccgtgctgc
     9421 tggccctggg cttcaacctg gcgcgccgct ggacccaggc acgggcggga ttgccatccg
     9481 gttagcttgc ctcgctgcga ttacggccga ccggcgggca cgcgcccacc ctggaccatg
     9541 agcgtgccgg cgcggccggg gacggtgccg ggctcgacgg tatggaacgg caggttcgca
     9601 acgtcgaggc gatcgaaaat ctgccgcatg gcaacgagtt cgtatccctg gctgcgccat
     9661 ccaccgatca gccgctcgag taccggcagc aactgcagtc cttccagctc ggcatggagc
     9721 gtgaacacgt gcgcctgcga cgccgggtcc tgcgcggtat ggcacagcag cagctggtcc
     9781 acgttggatg cgtcgcagcc ttgcgtgccg atcagttcgt ccagggtggg cagggtcgtg
     9841 ggcaattgcg ggcgcgtcat cggcgatcct tcgattaccg ggatgaaagg gtggcggccg
     9901 cgcgtgtccg atgcataatc gatcgggcct tgctcctcca gccggaacgc gtccgcgttc
     9961 atctgccagc cagccgcacc gtgcactcgg ggcagctcgc cgaatatctg cgcgtagcgg
    10021 tcgacgccgc gccgccactc ggtctcggtc cacgccgggc cccgcacggc gacgtagtct
    10081 tgccactcga catggtccca ggcgtgcaac cctgtttcga atcccgcggc ctgcaccgcc
    10141 cgcatttcct cccggcaggc ggcgccgatg tccgggccgg gcaggacggt tccgtaaagc
    10201 agcgttctca gcccgtaatg ccggatgacc gacgtgcgcg cgaccttgcc cagaaatccc
    10261 cgcttgaaca cccggcgcag cgcccggccg gtatggtccg gcccgacgct gaacaggaac
    10321 gtcgcccggg cattggcccg ttccagcgcg cgcagcaggc gcgggacacc ttcacgggtg
    10381 ccgcggtagg tgtcgaagtc aatcttgagc gccagcctca tgcgggcgtc gcagcgagcg
    10441 gaacggactc aggttaccaa gcgccgcgcg tccgggatgt gcccgcggta ggcgtcgtaa
    10501 atccgtttga gcgcctcgcg catcccgatc cgcgggcgcc agtcgagatc gcggcgggtg
    10561 acatctatct tcggcacccg gttctgcatg tcctggtagc ccgtgccgta atacttcgca
    10621 gcagtcgtgg tcccgatccg cacttttctc gcccgctccc ggtattcggg gtaggtcagc
    10681 gcaatttcga ccatcagctg ggccagctcc cggatcgaga ggttattctt cgggttgccg
    10741 atgttataaa tcttcccgct ggcgacgcgt ccggggttct cgataatttt catcagcgcg
    10801 tctatgccgt catcaacgta ggtgaaggag cggcgctgcc gcccgccgtc caccagcttg
    10861 atcgggtcgc cgcgcacgat ctggccgaga aactgggtga ccacgcgtga gctgccctcc
    10921 ttatcggcgt gcaggttgtc gaggccggga ccgatccaat tgaacggccg gaacagggtg
    10981 aaatcgaggc ggcgttccac gccataagcc cagatcaccc ggtccatgag ctgcttcgcg
    11041 caggagtaaa tccagcgctg cttgttgatc ggccccagca ccagctcgga agtctcggcg
    11101 tcgaactggc cgtcgcggca catcccgtac acttccgatg tcgacggaaa gaccagccgc
    11161 ttgtggtatt ccacgcaggc gcgcacgatc ggcaggttgg cctcgaaatc gagttcgaac
    11221 acccgcagcg gttctgcaac gtaggtggcc ggcgtggcga tcgccaccag cggcagcacc
    11281 gtgtcgcact tgcggatgtg gtatccgatc cattccttgt tgatggtgat gtcgccctcg
    11341 aagaaatgaa accgcttttc gcccatgaga tcggcgatcc gatcggtatt catgtccatg
    11401 ccgtaaatct cccacggcgt ggtttcgatg acacgcttcg acaggtggtg gccgatgaac
    11461 ccgtttactc cgagaatcag aattttcttc atggcccgcg tcaattgcga ggatgttgct
    11521 ggagcgggaa gggggcgcgg cggccgagga gcgcttcgag cgcctgcggc tgcacgccga
    11581 gcccgtccat ctcgagatcg agcaggcgca ggacgccgcc gtccgcgcag catgcaaagc
    11641 agccgttccg gtcgatatga atcgaaggcg ccgccggcgt gccgcattca cgttcgacgc
    11701 gcgtgcgcag tacgcgcaag ttgcggccgg ccagctgtgt gaatgcgccg gggtagggcg
    11761 gcgcgacccc gcgaaccagg ttgtggatcg cgtgcgcgcc cagtgtccag tcgatccgtc
    11821 cgtcctcggg gcgccgcgcg ccaaagtagg tcgccgacgc ctggtcctgc g
//
LOCUS       DBNGWZ010000336        15311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_544991, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000336 DBNGWZ010000000
VERSION     DBNGWZ010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_544991"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC16CX_09650"
     CDS             complement(<1..364)
                     /locus_tag="AC16CX_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3012813.1"
                     /translation="MKTSARRAAGKNSPNAVLRSTPETVSWGWIAADRPPVARVKSGQ
                     TVRIETVTHQGLNTGKDPVAFFGTAGIAPGEVLKDASEIYRSMKRGEGAGPHILTGPV
                     YVEGARPGDMLEVRVLDIE"
     gene            complement(387..989)
                     /locus_tag="AC16CX_09655"
     CDS             complement(387..989)
                     /locus_tag="AC16CX_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HIR3012814.1"
                     /translation="MAVKFEYPPGATPLDPDDAAGLIPSHIATQGQLNEWEFQNVAEG
                     RAWALSRKRKELLTADFARELHRRMFGKTWKWAGNFRKKETNPGIDPINIAVELKKLF
                     DDVAYQIKQKSMPLDEIAARFHHRLTQIHPFPNGNGRHARLMTDILLKLNGAEPFDWG
                     NSDLVASDEVRDRYIAALRAADGHDHSPLQEFVRSGRRNS"
     gene            complement(980..1441)
                     /locus_tag="AC16CX_09660"
     CDS             complement(980..1441)
                     /locus_tag="AC16CX_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein A"
                     /protein_id="HIR3012815.1"
                     /translation="MGQEYAKLKLKQTEELVQPFQRLAHAQTPRGGWVRAIREALGMS
                     AHQLGTRVHVKRQTIENLERSEASGKISLDSLNRVAKALGCRVVYALVPEKPLDEMQR
                     ERAQLIAESVVKPVSHSMRLEAQSISKREEQRQKEQLAQKLLQGNPKKLWQ"
     gene            complement(1782..2186)
                     /locus_tag="AC16CX_09665"
     CDS             complement(1782..2186)
                     /locus_tag="AC16CX_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012816.1"
                     /translation="MKTSARRAAGKNSPNAVLRSTPETVSWGWIAADRTPVARVKSGQ
                     TVRIDTVTHQGLNTGKDPVAFFGAADIAPGEVLKDTSEIYCSVKRGEGTRPHILTGPI
                     YVKTPRGQTRIFGAPVRRRAVAATTPVMSDEW"
     gene            complement(2231..5668)
                     /locus_tag="AC16CX_09670"
     CDS             complement(2231..5668)
                     /locus_tag="AC16CX_09670"
                     /EC_number="3.1.11.5"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR3012817.1"
                     /translation="MKTVSESPQGIETLRARDAKARCEALDIARSFIVQAPAGSGKTG
                     LLIQRYLALLARVDAPEEIVAITFTRKATAEMRARVLGALARAENDVPPAKPHERVTW
                     ELARAARERERAAKWGIAENPNRLRIQTIDSLCHWLTRELPLASGFGAQPETLEDADA
                     LYAEAAMRTLAGLDSKEPWARHVATVLKHLDNHVERAQVLIGGMLRVRDQWMRTVARA
                     HGRERAVLEAALANATRDALASLAALLPADARAEFPRLASYAAGNLPVEGNASPLTAC
                     RELRAMPGAAPDDLPAWRGIAELLLTKDGEPRRGVNARQGFPAATGAKGAERQRREDA
                     KKEFETLATKLAAHRTFLEQLHFTRSLPPPGYSDAQWEVVEALAALLPVAVGMLKVLF
                     QERGQVDFTEVSQRAVQALGEPDAPTDLALALDYRVRHVLVDEFQDTSWSQHDLLAGL
                     TVGWQPGDGRTLFVVGDPMQSIYRFRKAEVGLYLRAWHEGIGPVALERLVLSVNFRST
                     RAIVGWFNNVFEQVLPDEEDLASGAVLFAPSVADTDAPEGTAPRVHALLSTDRMAEAR
                     LVAGLVLEARAENPGEVALLVRSRAHLARIAPELKRAGVRFQAIEIEELRHRPVVQDL
                     LALTRALAHPADRVAWLSVLRAPWCGLTLADLDALAGHNHDAALWTLMNDAVILARLT
                     GDGRARLERLREALGAALARRRCGPFARWIEGAWVALGGPACAADATDIEDARVFFKL
                     VEELEEGGDLPALETLAERVAKLYALPDPGAEAARVQLMTVHKAKGLQFDTVILPGLG
                     YPPRRDDPKLLLWLERPRGHAEPDLLLAPIREASAERDAIYEYLKELESTKEKHETAR
                     LLYVAATRARSRLHLIGQVDWNEEGDARPRSRSLLEHLWPAVSNEFERAAGQAAATVD
                     LQAETPPHVIRRFVSGWRPPAPPAGLAWQPGAVPGYEGRSPHDEVEFSWASETAKHAG
                     TVVHRFLQLIAREGLERWDARRIGQLREVFARDLARLGVPEPERMGAVARVVDALASA
                     VAGERGRWVLGLHAEAGSELRLTGVIGDDIVSVAMDRTFVDERGARWIVDYKTGVHEG
                     GDVEAFLDRERERYRPQLERYAALMRGLDNRPIRLGLYFPLLGGWREWEYLPFDKHG"
     gene            complement(5652..8345)
                     /locus_tag="AC16CX_09675"
     CDS             complement(5652..8345)
                     /locus_tag="AC16CX_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3012818.1"
                     /translation="MRPDLAQRIEAGCVVVTPNRRLAAHLKREHDLAQAASGKTVWPS
                     ADCIPLNAVLERTYDELTHLAAGAALLSSQQEDVLWEQAVADSPQGVALLHPEAAARG
                     ARAAWQIQCAHRIELDRYRSALDEDGRAYLDWLEQYRKFLRAGKWLDAARVPEAIALA
                     LESGAEAAARPLVLYGFDQIAPQHRAVFDALAARGWQISEMAVQLRAGNARRVAYDDR
                     EAELTAVACQVREALANRPGTRIGVIVPDLAAARSDVQRIFDDALDPARVLGEARERP
                     RPYNVSLGLPLSDHPLVHTAFLILELARGELKLEQAGSLLRSPFIAAAEQELASRALL
                     DRDLRSDGRPLVELRALARRARGEGPHGTAGCRELAARLTAWAKLAADARKGRQSPSQ
                     WSTVFQRLLAGLGWPGERALDSGEYQTFAKWRETVSSLSALDLVAPRLAYDEALARLK
                     RITAETVFQPETPEVPVQVLGVLEANGLEFDRLFVTGLTDEAWPPAPRPNPFLPLALQ
                     RAHGVAHATGDWQLEYARRTTALWLGAAPEVRLSWPRRDGERALGMSPLLKPLVQAES
                     APSGVEGLLRNAIFAARALEEIGDFAAPRLPAGIDVKGGAEFFQNQAACPFRGFAAHR
                     LGARALEAARVGLDPRDRGGLVHAAAYNLWRELSSHARLAAATEGELKAAVGRAVAAA
                     AESMRRRRPDVMTESFAVLERGRVAALLLRLLELEKGRAPFTVMGREEPRPITVAGVS
                     VSTRLDRVDRLDGGGHVVLDYKTSRDVDLADWLGERPDEPQLPLYAASGRNEPAAVAF
                     VQLNARRVRFEGLSRAADVLPGVKLLAESRAAKLYPDWNAMMESWRAVLENLAREYLA
                     GRAAVAPKQYPETCKHCDFPTLCRVQELMDRGPVAGDENSE"
     gene            complement(8380..9636)
                     /locus_tag="AC16CX_09680"
     CDS             complement(8380..9636)
                     /locus_tag="AC16CX_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012819.1"
                     /translation="MKRTIGVIGSAAILSALPGLAVAQQQVVKPPVARYWMSVETAAG
                     MSMPGMAGMGSIMAGMMGGQAQSGRRMMLQLGSQRPSADSPRADHHIPPGMDMGASLP
                     LATPRREAAPRGGEAGERDLPQGMEQPRGRMLIYWGCGENVRAGQPVVIDFARVAQGQ
                     TPPNMVSRRVSVQTSPSPGRARTYGDWPNQQDSKAVPASASLKGDHRIAGNYTPDIQF
                     ALGDGYDFMDRVELSSSPRGAGARSVAWNAVPTATGYFATVMGAENQDEIIFWSSSEV
                     QEMGGMLMDYVPPAEVARLVREKVVMPAQQTECTVPAEVVKKAGGAPFLNFIAYGPEA
                     NFAQPPRPQDSKQPWEPLWTVKARFKSTASTLLGDAADGGRRRPARGEPAGEPREEQA
                     AQPAQKPAAPDPLKEGVNILRGIFGR"
     gene            complement(9932..10255)
                     /gene="fdxA"
                     /locus_tag="AC16CX_09685"
     CDS             complement(9932..10255)
                     /gene="fdxA"
                     /locus_tag="AC16CX_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HIR3012820.1"
                     /translation="MTYVVTETCIKCKYTDCVDVCPVDCFREGPNMLVIDPDECIDCT
                     LCVAECPVEAIFAEDDVPDKQKHWTKINAELAKEWKPIIEKKPAPPDADEWAKIKEKE
                     HLLEK"
     gene            10456..10782
                     /gene="trxA"
                     /locus_tag="AC16CX_09690"
     CDS             10456..10782
                     /gene="trxA"
                     /locus_tag="AC16CX_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HIR3012821.1"
                     /translation="MSEHIHYVTDQTFDPEVLQSPTPVLVDYWAEWCGPCKMIAPILD
                     EVAKEYSGRLKVAKLNIDENQATPPKYGIRGIPTLMLFKNGSVEATKVGALSKSQLTA
                     FIDSHI"
     gene            10913..12172
                     /gene="rho"
                     /locus_tag="AC16CX_09695"
     CDS             10913..12172
                     /gene="rho"
                     /locus_tag="AC16CX_09695"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR3012822.1"
                     /translation="MHLSDLKSLHVGELLEMAIKNEVDGANRMRKQELIFALLKNQAK
                     KGESIYGGGTLEVLPDGFGFLRSPDTSYLAGTDDIYVSPSQIRRFNLHTGDTIEGEIR
                     TPKDGERYFALVKVDKVNDEPPEASKHKILFENLTPYHPTEHLKLERDIKAEENITAR
                     IIDIIAPIGKGQRGLIVSPPKAGKTVLMQQLAHAITANHPAVVLIVLLIDERPEEVTE
                     MIRSVKGEVVSSTFDEPAARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALQRPKRFFGAARNMEEGGSLTILATALIDTGSRMDDVIYEE
                     FKGTGNMEIHLDRRMYEKRIFPAINVNRSGTRREELLIKQDVLQKIWVLRKLLYPMDE
                     LEATEFLLDKIRVTKGNADFFDSMRRG"
     gene            12580..13002
                     /locus_tag="AC16CX_09700"
     CDS             12580..13002
                     /locus_tag="AC16CX_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012823.1"
                     /translation="MIWRVLLIAVLLAGCAQLPPTPQDIQAKKFETVPDKAVIYLVRD
                     HPDFSDRAATIWLGDAAMITTYPGTYFRWEVAPGTHRITGYGADIGTITLHAEASRIY
                     YVQQQLSPFLRYPQSYFYLVSEPYGRSAVMRAVLVGGQ"
     gene            13002..13484
                     /locus_tag="AC16CX_09705"
     CDS             13002..13484
                     /locus_tag="AC16CX_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012824.1"
                     /translation="MRASRHLLGYARIASAAAALALGGCASTPEATSERDAEAKRFIV
                     HPTASTLYVYRNDFPADIDMHDTVLYVDGRIIGATLAGTFFRIDVRPGEHLLHGFAFD
                     QGRLKIAAGSGELVFVSLNVANGTSRFARVSPEAGKRQIARCCVLLENWAQGQRPLLR
                     "
     gene            13613..13840
                     /gene="rpmE"
                     /locus_tag="AC16CX_09710"
     CDS             13613..13840
                     /gene="rpmE"
                     /locus_tag="AC16CX_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3012825.1"
                     /translation="MKPKIHPQYSTITVTCSCGNTWETRSTLGRDLHVEVCSVCHPFY
                     TGKQKIVDTAGRVERFRQKYARKTPAKTASA"
     gene            complement(13925..14380)
                     /locus_tag="AC16CX_09715"
     CDS             complement(13925..14380)
                     /locus_tag="AC16CX_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012826.1"
                     /translation="MNKIACFSTLLIVLAATALASAAEPELNAVLSVMGYGHKVKVLV
                     NGADTGVTGGKSQSMRLFNKGHPWSAKATADMRARNFVLVPGANEVTIEFTRDPKAND
                     RLTIELEAEGYPEPLLKLVNQGKSSDKYIVKLHIEPKAPKGFKPVVIKD"
     gene            complement(14430..>15311)
                     /locus_tag="AC16CX_09720"
     CDS             complement(14430..>15311)
                     /locus_tag="AC16CX_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3012827.1"
                     /translation="PLALEHSLGTVYNLPPPTQGLVSLLILGMLDALEVGSTGPASAD
                     YVHLAVEATKRAFGVRDHCITDPAYMTVDAQALLAPGKIRELAANVDRGRAARWGAGK
                     PPADTVWMGVVDGSGRAVSMIQSLYHEYGSGVVLEETGINWQNRGCSFSLDTAALNSL
                     RSGRKPFHTLNPALALLRDGRTLVYGTMGGDGQPQTQSAVFTRIAVFGMDPQQAIAAP
                     RWLLGRTWGQTSDSLKLESRFSTGVVADLARRGHVMEVLADYDETMGHAGAIVRHADG
                     VLEGGADPRSDGAVAAF"
BASE COUNT         2410 a         5270 c         4965 g         2666 t
ORIGIN      
        1 tctcgatgtc gagcacgcgc acttcgagca tgtcgcccgg ccgcgcgccc tcgacgtaga
       61 ccgggccggt gaggatgtgc ggtcccgcgc cttccccgcg cttcatgctg cggtagatct
      121 ccgaggcatc cttcagcacc tcgcccggcg cgatgccggc cgtcccgaaa aacgcgacgg
      181 gatccttgcc ggtgttgagc ccctggtgcg tgaccgtctc gatcctgacg gtctgcccgg
      241 atttcacgcg cgcgaccggc gggcgatcag cggcgatcca gccccagctc acggtctcgg
      301 gggtagagcg cagaactgcg ttcggcgagt tttttccagc ggcgcggcgc gcgctcgttt
      361 tcatggctgc tcgattctag ttcgcatcat gaattcctgc gtcctgatcg cacgaattct
      421 tgcaacggcg agtgatcgtg gccgtcggct gcgcgcagcg cggcgatata gcgatcgcga
      481 acctcatcgg acgcaacgag gtcactgttc ccccaatcga acggctcggc accgtttaac
      541 ttgagcagga tatccgtcat caggcgcgca tgacggccgt tgccgttggg aaaaggatga
      601 atctgcgtga gccggtgatg aaatctggcc gcgatttcat cgagtggcat cgatttctgc
      661 ttgatctgat aggcaacgtc atcgaagagc ttcttgagct cgacggctat gtttatcggg
      721 tcgatgcccg ggttggtttc tttctttcgg aagttgcctg cccatttcca ggtcttcccg
      781 aacatccttc gatgaagctc ccgcgcaaag tcggccgtca ataactcctt tcgcttcctc
      841 gacagagccc atgccctccc ctccgctacg ttctggaact cccattcgtt gagctggcct
      901 tgggtcgcga tatgcgatgg aatcaggcct gccgcgtcgt cggggtcgag tggggtcgcg
      961 cccggcgggt attcgaattt cactgccata gctttttcgg gttcccctgg agcagttttt
     1021 gagccaactg ctctttctgg cgttgctctt cccgcttgct gatcgactgc gcttcgagcc
     1081 tcatcgagtg tgacacgggc ttcacgacgg attcagcgat aagctgagcg cgctcgcgct
     1141 gcatctcatc gaggggcttc tccggtacaa gcgcataaac gacgcggcat ccgagtgcct
     1201 tggcgactct gttcagactg tcgagcgaga ttttcccgct cgcttcactg cgctccaggt
     1261 tttcaatcgt ttgccgcttc acgtgtacgc gcgtgccgag ctggtgcgcg gacatgccga
     1321 gcgcctcgcg gatcgcgcgc acccagccgc cgcgcggcgt ctgcgcgtgc gcgagccgtt
     1381 ggaatggctg caccagttcc tcggtctgct tgagcttcaa cttcgcatat tcctgtccca
     1441 tgctttcctc atctggcagc atataggctg ccgcgatgga ttattatgca ggatataagc
     1501 tgccgatgca acttataagc gcagggtata agctgccgca tgcggggagg gccgtggcga
     1561 tgatgcccac gcgatggagc ctgcttccgg tggcagccgt ttgtcttaga gcgtcgtttt
     1621 caccgcgaaa aacgcgtcgt taagcgcgaa aaaggccacg attcggcgtt cttgttttgc
     1681 aggatcaaac tgttgcgttg gtccgacccg ggcggtaaac cgccggttta acttcgcgtg
     1741 ttcgaacgga caagtggcgc gccgttcaac acgcgacggg ctcaccactc atcactcatt
     1801 accggggtcg tagctgcgac tgctcggcgg cgaacagggg ccccgaaaat tcgagtctga
     1861 ccccgaggcg tcttgacgta gatcggtccg gtgaggatgt gcggcctcgt gccttccccg
     1921 cgcttcacgc tgcagtatat ttccgaggta tctttcagca cctcgcccgg cgcaatgtcc
     1981 gccgccccga aaaacgcgac gggatccttg ccggtgttga gcccctggtg cgtgaccgtg
     2041 tcgatcctca cggtctgccc ggatttcacg cgcgcgaccg gcgtccggtc cgcggcgatc
     2101 cagccccagc tcacggtctc gggggtagag cgcagaactg cgttcggcga gttttttcca
     2161 gcggcgcggc gcgcgctcgt tttcatggct gctcgattct agttctcact tagccctgaa
     2221 gggctgaacc ctaaccgtgt ttatcgaagg gtaaatattc ccactccctc catcctccga
     2281 ggagggggaa atacagcccc aaccggatgg gccggttgtc caacccgcgc atgagcgccg
     2341 cgtagcgctc gagctgcggc cggtagcgct cgcgctcgcg atcaaggaag gcctcgacgt
     2401 ccccgccctc gtgcacgccg gtcttgtagt ccacgatcca gcgcgcgccg cgctcatcca
     2461 cgaaggtgcg gtccatggcg acgctcacga tgtcgtcccc gatcacgccg gtcaggcgca
     2521 gctccgaccc ggcttccgcg tgcaagccca gcacccagcg cccgcgctcg ccggcgacgg
     2581 ccgaggcgag cgcgtccacg acgcgcgcga cggctcccat ccgctccggc tccggcacgc
     2641 cgagccgcgc gaggtcgcgc gcgaagactt cgcgcagctg cccgatgcgc cgcgcgtccc
     2701 agcgctcgag tccttcccgc gcgatcagct gcaggaagcg gtgcacgacc gttcccgcgt
     2761 gcttcgcggt ctccgacgcc cacgagaact cgacttcgtc gtgcgggctc cgcccctcgt
     2821 agccgggcac ggcacccggc tgccaggcga ggcccgccgg cggcgcaggc ggccgccagc
     2881 cggacacgaa gcggcggatc acgtggggcg gcgtttcggc ctggagatcg accgtggcgg
     2941 cggcctgccc ggccgcccgc tcgaactcgt tcgaaaccgc cggccataga tgttcgagca
     3001 gcgatcgcga tctgggcctg gcgtcgccct cctcgttcca gtccacctgg ccgatgaggt
     3061 gcagacgcga tcttgcgcgg gtcgccgcca catagagcag ccgcgcggtc tcgtgcttct
     3121 ccttggtgga ctcgagttcc ttcaagtatt cgtagatcgc gtcacgctcg gcgctcgctt
     3181 cgcggatcgg agcgagcagg agatcgggct cggcgtgacc gcgcggtcgc tcgagccaca
     3241 gcaggagctt ggggtcgtcg cgccgcggcg gatagccgag ccccggcagg atcacggtgt
     3301 cgaactgcag ccctttcgcc ttgtgaacgg tcatgagctg cacgcgtgcc gcctccgcgc
     3361 cggggtcggg cagcgcgtag agcttcgcaa cccgctccgc gagcgtctcg agcgcgggaa
     3421 ggtctcctcc ttcctcaagc tcctcgacaa gcttgaaaaa tacgcgcgcg tcctcgatgt
     3481 cggtcgcatc cgcggcgcac gcggggccac cgagcgcgac ccacgcgccc tcgatccagc
     3541 gggcgaatgg cccgcagcga cggcgggcga gcgccgcacc gagtgcttcg cgcaagcgtt
     3601 caaggcgtgc tcttccgtcc ccggtaagcc gcgccaggat cacggcatcg ttcatgagcg
     3661 tccacagcgc ggcgtcgtgg ttgtgtcccg ccagcgcgtc gaggtcggcg agcgtgagcc
     3721 cgcaccacgg cgcgcgcagc accgagagcc acgcgacgcg atcggccggg tgcgcaagcg
     3781 cgcgcgtgag cgcgagcagg tcctgcacga ccgggcggtg gcgcagttcc tcgatctcga
     3841 tcgcctggaa gcggacgccg gcgcgcttga gttccggcgc gattctcgcg agatgggcgc
     3901 ggctcctcac gagcagcgcc acctccccgg gattttccgc gcgcgcctcg agcacgaggc
     3961 cggcgacgag ccgggcctcc gccatccggt cggtggaaag cagcgcgtgc acgcgcgggg
     4021 cggttccttc tggcgcgtcg gtgtcggcga ccgagggcgc gaacagcacc gcgccgctcg
     4081 ccaggtcttc ctcgtccggc agcacttgct cgaatacatt gttgaaccag cccacgatcg
     4141 cgcgcgtcga gcggaagttg acggagagca cgaggcgctc cagcgccacc ggcccgatgc
     4201 cctcgtgcca ggcgcgcagg tagaggccga cctccgcctt gcggaagcgg tagatggact
     4261 gcatcgggtc gcccaccacg aagagggtac ggccgtcgcc cggctgccag ccgacggtga
     4321 gcccggcgag gagatcgtgc tggctccagg aggtgtcctg gaactcgtcc accaggacgt
     4381 gccggacccg gtaatccagc gcgagcgcga ggtcggtggg cgcatccggc tcgccgagcg
     4441 cctgcacggc gcgctgcgag acttcggtga agtccacctg cccgcgctcc tggaacagca
     4501 ccttgagcat cccgaccgcg accggcagca gcgccgccag cgcctcgacc acctcccact
     4561 gcgcgtcgct gtagcccgga ggcggcaggc tgcgcgtgaa gtgcagctgc tcgaggaagg
     4621 tgcggtgagc cgcgagcttc gtcgccagcg tttcgaattc cttcttcgcg tcctcgcgcc
     4681 gctggcgctc cgcgcccttc gcgccggttg ccgcggggaa gccttgcctc gcgttcacgc
     4741 ccctgcgcgg ctcaccgtcc ttcgtgagca gcagttccgc gattccccgc caggcgggaa
     4801 gatcgtcggg cgcggcgccc ggcatcgcgc gcagctcccg gcaggccgtg agcggtgagg
     4861 cgttcccctc taccgggaga ttcccggcgg cgtacgacgc gagccggggg aactcggcgc
     4921 gcgcatccgc cggtaggagc gcggcgaggc ttgcgagcgc gtcgcgcgtt gcgttcgcga
     4981 gcgccgcctc cagcacggct cgctcgcgtc cgtgcgcgcg tgcgaccgtg cgcatccact
     5041 ggtcgcgcac ccgcagcatg ccgccgatga gcacctgcgc gcgctcgacg tggttgtcga
     5101 ggtgcttgag cacggtcgcg acgtggcgcg cccacggctc cttcgagtcg agaccggcga
     5161 gcgttcgcat cgccgcttcg gcgtagagcg cgtccgcgtc ctccagcgtc tcgggttgcg
     5221 cgccgaagcc ggaggcgagc ggcagctcgc gcgtgagcca gtggcagagc gagtcgatgg
     5281 tctggatgcg cagccggttg ggattctcgg cgatgcccca tttcgccgcg cgttcgcgct
     5341 cgcgtgcggc acgggccagc tcccaggtca cgcgctcgtg cggtttcgcg ggcgggacat
     5401 cgttctctgc gcgggcgagc gcccccagca cgcgcgcgcg catctccgcc gtcgccttcc
     5461 tggtgaaggt gatggcgacg atctcctcgg gtgcgtccac gcgcgcgagc agcgcgagat
     5521 agcgctggat gagaaggccg gtcttgcccg aacccgccgg cgcctgcacg ataaaggagc
     5581 gcgcgatgtc gagcgcctcg catcgcgctt tggcgtcccg cgcgcgcaac gtttcgatgc
     5641 cttgcggcga ctcactcact gttttcatcc cccgccaccg gcccgcggtc catgagctcc
     5701 tgcacgcggc agagcgtcgg gaaatcgcag tgcttgcagg tttcggggta ttgcttcggc
     5761 gccaccgccg cgcggccggc gagatattcg cgcgcaaggt tttccagcac cgcgcgccag
     5821 ctttccatca ttgcgttcca gtccgggtag agctttgccg cccgggattc cgccagcaac
     5881 ttgacgccgg gcagcacgtc ggcggcgcgc gacagtccct cgaacctcac ccggcgcgcg
     5941 ttgagctgta cgaacgcaac cgcggctggc tcgttgcgcc cactcgcggc atagagcggc
     6001 agctgcggct cgtcggggcg ctcgccgagc cagtcggcga ggtccacgtc gcggctcgtt
     6061 ttgtagtcga gcacgacgtg gccgccgcca tcgagccggt ccacccggtc gagccgcgtc
     6121 gagacgctta cgcccgcgac ggtgatcggc cgcggctctt cgcgccccat caccgtgaac
     6181 ggcgcgcggc ccttctccag ctcgagcaga cgcaagagca gcgccgccac gcgcccacgc
     6241 tcgagcaccg cgaaagattc ggtcatgacg tcgggacggc gtcggcgcat gctctccgcc
     6301 gccgccgcaa cggcacgccc gaccgcggcc ttgagctccc cctcggttgc cgcggcgagg
     6361 cgggcgtggc tactcagctc gcgccacagg ttgtaggccg ccgcgtgcac cagcccaccg
     6421 cggtcgcgcg ggtcgaggcc gacgcgcgcc gcctccagcg cacgcgcgcc caggcggtgc
     6481 gctgcgaagc ccctgaaagg acaggccgcc tggttctgga agaattccgc gccgcccttc
     6541 acgtcgatgc ccgctggaag ccgtggcgcg gcgaaatccc cgatctcctc gagcgcgcgc
     6601 gcagcgaaga ttgcattgcg cagcaaccct tcgactccgc tcggggcaga ctcggcttgc
     6661 acgagcggct tcagcagcgg gctcatgccg agggcgcgct cgccgtcgcg gcgcggccag
     6721 gagagcctca cttccggcgc ggcgccaagc cagagggcgg tggtgcggcg cgcatactcg
     6781 agttgccagt cgccggtcgc gtgcgcgacg ccgtgcgcgc gttgcagcgc gagcggcagg
     6841 aacggattgg gacgtggcgc gggcggccac gcctcgtcgg tgagaccagt gacgaagagc
     6901 cggtcgaatt cgagcccgtt cgcctccagc acgccgagca cctgcaccgg cacctccggc
     6961 gtttccggct ggaacaccgt ctccgcggta atgcgcttca ggcgcgcgag cgcctcgtca
     7021 taggcgagcc gcggtgcaac gaggtcgagc gcggagagcg aagaaacggt ctcccgccac
     7081 ttggcgaagg tctggtactc cccggaatcg agtgcgcgct cgcccggcca gccgaggccc
     7141 gcgagcagcc gctggaacac cgtgctccac tgtgaaggcg attgcctgcc cttgcgcgcg
     7201 tcggcggcga gcttggccca cgccgtcagg cgcgcggcga gttcgcgaca gcccgcggtg
     7261 ccgtgcgggc cttcgccgcg cgcacggcgc gcgagcgcgc gcagctcgac gagcggccgg
     7321 ccgtcgcttc tcaagtcccg gtcgagcagc gcgcggcttg cgagttcctg ctccgccgcc
     7381 gcgatgaagg ggctgcgcag gagcgagccc gcctgctcga gcttgagctc cccgcgcgcg
     7441 agctcgagga tgaggaacgc ggtgtggacg agcgggtggt ccgaaagcgg cagcccgagc
     7501 gagacgttgt agggccgcgg gcgctcgcgc gcttccccca gcacccgcgc gggatcgagc
     7561 gcgtcgtcga agatgcgctg cacgtcgctg cgcgccgccg caagatcggg cacgatgacg
     7621 ccgatgcgcg tgccgggacg gttcgcgagc gcttccctca cctggcacgc taccgccgtg
     7681 agttccgctt cgcggtcgtc gtaggcgacg cgccgggcgt tccccgcgcg cagctgcaca
     7741 gccatttccg agatttgcca gccgcgcgcc gccagcgcgt cgaagaccgc gcggtgctgc
     7801 ggcgcgatct ggtcgaagcc atagagcacg agcgggcgcg cggcggcctc tgcgccgctt
     7861 tccagcgcca gcgcgatggc ctccggcacg cgcgccgcgt cgagccattt gccggcgcgc
     7921 aggaatttgc ggtactgctc caaccagtcg agataggcgc ggccgtcttc gtcgagggcg
     7981 gagcggtagc gatcgagctc gatgcggtgg gcgcactgaa tctgccacgc cgcgcgcgcg
     8041 ccgcgcgccg ccgcttcggg gtggagcagc gcgacgccct gcggcgagtc ggcaacggcc
     8101 tgctcccaca gcacgtcctc ctgctgggag gaaagcagcg ccgcgcccgc ggcgaggtgc
     8161 gtcagttcgt cgtacgtgcg ctcgagaacc gcgttaagcg ggatgcagtc ggccgagggc
     8221 cacaccgtct tgccggacgc ggcctgcgcg agatcgtgct cgcgcttgag gtgcgcggcg
     8281 agcctgcggt tgggagtgac gaccacgcac ccggcttcaa tccgctgcgc gaggtcgggt
     8341 cgcatggtgc tacatggcgt gaggcgcccg cagcggcgtt cagcggccga agatgccgcg
     8401 caggatgttg acgccttcct tcagcggatc gggagcggcc ggcttctgcg ccggctgggc
     8461 ggcctgctcc tcgcgcggtt cgcccgcggg ctcgccgcgc gccgggcggc ggcggccgcc
     8521 atcggccgcg tcgccgagca gcgtcgaggc ggtggatttg aagcgcgcct tcaccgtcca
     8581 cagcggctcc cacggctgct tcgaatcctg cgggcgcggc ggctgcgcga aattcgcttc
     8641 cggaccgtag gcgatgaaat tgaggaaagg cgcgccgccc gctttcttca ccacttcggc
     8701 gggtaccgtg cactcggtct gctgcgcggg catgaccacc ttctcccgca ccaggcgcgc
     8761 gacttccgcg ggcggcacgt agtccatgag catgccgccc atctcctgaa cttcgctcga
     8821 cgaccagaag atgatctcgt cctggttctc ggcccccatc acggtcgcga agtagccggt
     8881 cgctgtcggc accgcgttcc aggcaacgct gcgcgcgccc gcgccgcgcg gcgaggagga
     8941 gagctcgacg cggtccatga aatcgtagcc gtcacccagc gcgaactgga tatcgggcgt
     9001 gtagttgccg gcaatccggt gatcgccctt gagggaggcg cttgcgggca ccgctttcga
     9061 atcctgctgg ttcggccagt cgccgtaggt cctggcgcgc ccgggcgacg gcgaggtctg
     9121 cacggagacg cggcgcgaga ccatgttggg cggcgtctgt ccctgcgcga cgcgggcgaa
     9181 gtcgatgacg accggctgcc ctgctctcac gttctcgccg cagccccagt agatgagcat
     9241 gcgcccccgc ggctgctcca tgccttgcgg caggtcgcgc tccccggcct cgccgccgcg
     9301 cggtgccgct tcgcgtcgcg gcgtggcgag cggaagcgac gcgcccatgt ccatccccgg
     9361 cggaatatgg tggtcggcac ggggactgtc cgcagacggg cgctgcgagc cgagctgcag
     9421 catcatcctg cgaccgctct gcgcctgccc gcccatcatg ccggccatga tcgagcccat
     9481 gcccgccatg cccggcatgc tcatgccggc ggcggtctcg acgctcatcc agtagcgcgc
     9541 gaccggcggc ttcacgacct gctgctgcgc aaccgcgagt ccgggcagag cggaaaggat
     9601 cgcggcagag ccaatgactc caatggttct cttcatactc gggactcgcg gttcaaagtt
     9661 caaggtttca agttccaagt tcaaggttct aagttccaag taaccaacct ggaacattga
     9721 acttggaacc tgcgctagtg cggtggggtg cgtccactga gcacgtcccg atgcagattg
     9781 cgttgacgcc ggctatttct caagaagagg ctccttctct ttaatcttcg cccactcgga
     9841 agcgtggcgc gcggagggtt gagcaggggc cccgaactta ttcgtggatg cgaccccgga
     9901 gcgcgccccg acgcggattc gacgacgccg gctatttctc aagaagatgc tccttctctt
     9961 ttatcttcgc ccactcatcc gcgtcgggcg gggcgggttt cttttcgatg atcggcttcc
    10021 actccttggc aagctcggcg ttgatcttgg tccagtgctt ctgcttgtcg ggcacgtcgt
    10081 cctcggcgaa aatcgcctcc accgggcatt ccgcgacgca cagggtgcag tcgatgcact
    10141 cgtcgggatc gatgacgagc atgttggggc cttcgcggaa gcaatccacc gggcatacat
    10201 caacgcaatc ggtatacttg catttaatgc acgtctcggt gacgacgtaa gtcatagtat
    10261 tccttcggaa tttcggtccg gcttgaaatc acgcgggaga gtccatattt taacagacat
    10321 gtccggtcgt gccgatgcac ggacttgaca ttggccctat gcaaagcagg gaaatttcgc
    10381 tagcatagtg gggtaagtct cgctgccaca ccttcccgtg gcgttttccg acagatctcc
    10441 gagggtattg ttttcatgag cgaacatatc cattacgtga ccgaccagac cttcgatccc
    10501 gaagtgctcc agtcgccgac gccggtgctg gttgactact gggcagagtg gtgcggcccg
    10561 tgcaagatga tcgcccccat tctcgacgag gtggcgaagg agtactccgg gcggctcaag
    10621 gtcgccaagc tcaacatcga cgagaaccag gccacgccgc cgaaatacgg catccggggc
    10681 atccccacgc tgatgctgtt caagaacggc tccgtggagg ccaccaaggt cggcgccctc
    10741 tccaaatccc aactgaccgc ctttattgac agccacatct aaagctccta acctttcccg
    10801 ctagcgtccg ggcggcagcc gcccggcccc gcagcaagac acgggcgccc cggacgcggg
    10861 tgccgtttct cccctttttt ccccggtttc catccacaaa gagtcgcagt ttatgcattt
    10921 atcagatctc aagagcctcc acgtcggcga gctgctggaa atggccatca agaacgaggt
    10981 ggacggcgcc aaccgcatgc gcaagcagga gctgatcttc gcgctcctca agaaccaggc
    11041 gaagaagggg gaaagcatct acggcggcgg cacgctcgag gtgctgcccg acggcttcgg
    11101 gttcctgcgc tcgccggaca cctcctatct cgcgggcacc gacgacatct acgtctcgcc
    11161 gtcccagatc cgccgcttca acctgcacac cggcgacacg atcgagggcg agatccgcac
    11221 gcccaaggac ggcgagcgct acttcgcgct ggtgaaggtc gacaaggtca acgacgagcc
    11281 gcccgaggcc tccaaacaca agatcctgtt tgagaatctc accccctacc atccgaccga
    11341 acacctcaag ctcgagcgcg acatcaaggc cgaggagaac atcactgcgc gcatcatcga
    11401 catcatcgcg cccatcggca agggccagcg cgggcttatc gtctcgcccc ccaaggccgg
    11461 caagaccgtg ctgatgcagc agctcgcgca tgccatcacc gccaatcacc ccgccgtggt
    11521 gctgatcgtg ctgctgatcg acgagcggcc cgaggaagtg acggagatga tccgctcggt
    11581 caagggcgag gtggtgtcct cgacgttcga cgagccggcc gcacggcacg tgcaggtcgc
    11641 cgagatggtg atcgagaagg cgaagcggct ggtcgagcac aagaaggacg tggtgatcct
    11701 gctcgattcg atcacgcggc tcgcgcgcgc ctacaatacc gtggtccccg cctccggcaa
    11761 ggtgctcacc ggtggcgtgg acgcaaacgc cctgcagcgc ccgaagcgct tcttcggcgc
    11821 cgctcgcaac atggaggaag gcgggtcgct cacgatcctc gcgaccgcgc tgatcgacac
    11881 cggcagccgc atggacgacg tgatctacga ggaattcaag ggcaccggca acatggagat
    11941 ccatctcgac cggcgcatgt acgagaaacg catcttcccg gcgatcaacg tcaaccgctc
    12001 cggcacccgc cgcgaggagc tgctgatcaa gcaggacgtg ctgcagaaga tctgggtgct
    12061 gcgtaagctg ctctatccca tggacgagct cgaggccacc gaattcctgc tcgacaagat
    12121 ccgggtgacg aaaggcaacg cggacttctt cgatagcatg agaaggggct aatcaatttt
    12181 gtcagcctcg ccagatccat cgctttgtgg tccgaagtcc tgcccgcctc cacggtcgca
    12241 tccccgggaa ccggggcccc tgctccgccc tccgacgccg cggcctctga tcggtaatcg
    12301 gcaaggattc ggagaaatcg catcggccgc ttgccccgca gcctccgaac cgcgggtgac
    12361 aagtatctaa acttgtagtg tcggctgctt gcgcgcctcc gcggtcgcac ccgcaagcgg
    12421 gcacctgctc aacgctccgg cgcgctctcc ttgggtcgca tctccatcta gaaagaatgg
    12481 gacccctgcc tcttcgtaac atctccttac agactcaaga cctttccccc gcccgaagcg
    12541 tctaaactgg cagcgggccg ttgatccttg gggggtccca tgatctggcg agttttgttg
    12601 attgccgtcc tgctcgcggg gtgcgcgcag ctgccgccca cgccgcagga catccaggcg
    12661 aagaagttcg agaccgtccc ggacaaggcg gtgatctacc tggtgcggga ccaccccgat
    12721 ttcagcgacc gcgccgcgac gatctggctg ggggacgccg ccatgatcac gacctacccg
    12781 ggcacgtatt tccggtggga ggtggcgccg gggacgcatc gcattaccgg ctacggcgct
    12841 gacatcggca cgatcacgtt gcacgccgag gccagcagga tctactacgt gcagcagcag
    12901 ctgtccccgt tcctgcggta tccgcagtct tacttctacc ttgtcagcga gccctacggg
    12961 cgcagcgcgg tcatgcgcgc ggtgctggtg ggaggccaat gatgcgcgcg tcccgacacc
    13021 tgcttggtta cgcgcggatc gcgtcagcgg ctgcggcgct cgcgctcggc ggttgtgcgt
    13081 caacgcccga ggcgacctcc gagcgcgacg ccgaagcgaa gcgcttcatc gtgcacccca
    13141 cggcttccac cctctacgtc taccgcaacg actttcccgc cgacatcgac atgcacgata
    13201 ccgtgcttta cgtggacggc cgcatcattg gcgcaacgct ggccgggact tttttccgca
    13261 tcgacgtgcg cccgggcgag cacctgctgc acggctttgc cttcgaccag ggtcgcctca
    13321 agatagccgc cggctcgggg gagctcgttt tcgtgtcgct caacgtggcg aacggcacct
    13381 cgcgcttcgc gcgggtcagc cctgaagccg gcaagcgcca gatcgcgcgc tgctgcgtgc
    13441 tgctggagaa ctgggcgcag ggccagcgac cgttgctccg ctgaaggcgg aaggcgaaag
    13501 tcggaaggcg gaagtgttcg ttttcctttc gacattcgcc tttcgccttt cgccttccac
    13561 aatttagccg tgcttgccag cggcggctga attgtggata attcgctccc ttatgaaacc
    13621 gaagatccat cctcaataca gcaccatcac cgtcacctgc agctgcggca acacctggga
    13681 gacacgctcc acgctcggcc gcgacctgca cgtggaagtg tgctcggtgt gccacccgtt
    13741 ctataccggc aagcagaaga tcgtggacac cgccggccgc gtcgagcggt tccgccagaa
    13801 gtacgcgcgc aagacgcctg cgaagacggc aagcgcgtaa tcgtcacccc tcgcaaacaa
    13861 gaaagggcgg ccctggccgc cctttttgct ttacgccctc gcgggaactg cgcaccggtc
    13921 tcttctaatc cttgatcacc accggcttga agcccttcgg cgctttcggc tcgatgtgca
    13981 gtttcacgat gtacttgtcc gaagacttgc cctgattaac cagcttcagc agcggctcgg
    14041 gatagccctc cgcctccagc tcgattgtga gccggtcgtt cgctttcggg tccctggtaa
    14101 attcgatcgt cacctcgttc gcgccgggga cgaggacgaa gttgcgggcc cgcatgtcgg
    14161 ccgtggcctt ggccgaccac gggtgcccct tgttgaacag gcgcatgctc tgcgatttgc
    14221 cgccggtgac ccccgtgtcg gcgccgttga cgagcacctt gaccttgtgg ccgtagccca
    14281 tcaccgacag caccgcgttg agttcgggct cggccgcgct cgcgagcgca gtcgccgcca
    14341 gcacgatcag cagcgtggaa aaacacgcaa tcttgttcat gggtcttcct ttcgccggca
    14401 tcataccgca catcggatag cacgcaacgc tagaacgccg cgaccgcgcc gtcgctgcgc
    14461 gggtcagcgc ccccctcgag cacgccgtcg gcatgccgca cgatcgcccc ggcgtgtccc
    14521 atggtctcgt cgtaatcggc aagcacctcc atcacgtggc cgcgccgcgc gaggtcggcg
    14581 acgaccccgg tggaaaagcg cgactcgagc ttgaggctgt cgctcgtctg gccccaggtg
    14641 cgccccagca accagcgcgg cgcggcgatc gcttgctgag ggtccatgcc gaacacggcg
    14701 atccgcgtga ataccgcgct ctgtgtctgc ggctggccgt ccccacccat cgtgccgtag
    14761 acaagggtac gcccgtcgcg cagcagcgcc agcgccgggt tgagcgtatg gaagggcttg
    14821 cgcccggacc tgaggctgtt cagcgccgcg gtatcgagag aaaagctgca gccccggttc
    14881 tgccagttga tcccggtttc ctccagcacc acgccgcttc cgtattcgtg atagaggctc
    14941 tgtatcatgc tgaccgcgcg cccgctgccg tccacaacgc ccatccacac cgtgtccgca
    15001 ggcggtttgc ccgcgcccca tcgcgcggcc ctgccgcgat cgacgtttgc cgccagctca
    15061 cggatttttc ccggggccag cagcgcctgc gcgtcgacgg tcatgtaggc aggatcagtg
    15121 atgcaatggt cgcgcacgcc gaaagcacgc ttggtcgcct cgaccgcgag atgcacgtag
    15181 tccgcgctcg ccggtcccgt ggagccgacc tccagcgcgt cgagcatgcc caggatcagc
    15241 agcgatacca ggccctgggt cggcggcggc aggttgtaga ccgtgccgag cgagtgctcg
    15301 agcgcgagcg g
//
LOCUS       DBNGWZ010000337         7165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2810169, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000337 DBNGWZ010000000
VERSION     DBNGWZ010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2810169"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="AC16CX_09725"
     CDS             complement(<1..942)
                     /locus_tag="AC16CX_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012828.1"
                     /translation="MIRPIKSIVVALFIGLCHSAAAQTYPTKPIRVIVPFPAGESIDA
                     TARLIAQQWQTALGQQMVIDNRGGAGGTIGTEMTAKATPDGYTISYGNLGPLSIGPNL
                     YQKLGYNLFKDLAPVSQATSLPFVLFGSTTLPVTTARELVAYAKARPGQLNFGSTGVG
                     SGLHLMGELFKLTADVNIVHVPFKGVAQALPEMAAGRIQLAFNTIPAFLPHVKAGRLK
                     AFVITAQKRSPLLPDVPACTEVGLAGLDASAWHAVVAPAGTPKEIIRKLHQTLAATLA
                     QPDVRKQLAAVGAEPVASTPEEFAKFMRAESQKWARVI"
     gene            complement(967..2280)
                     /locus_tag="AC16CX_09730"
     CDS             complement(967..2280)
                     /locus_tag="AC16CX_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3012829.1"
                     /translation="MPVAARQRARALNEFTATEIVAEVASGRASCEAVAHACLERIAA
                     REPQVLAWAHLDTDHVIAQARALDRGARRGPLHGVPFGVKDIIDTCDLPTEYGTPIHK
                     GYQPAKDAACVALSRKAGGVLMGKTVTTEFANITPGPTRNPLDLSRTPGGSSSGSAAA
                     VADFHVPLAIGTQTTGSTTRPASFCGVFGYRPTYGDLRTAGVMEASGSLDTLGILARS
                     IDDVALYRDVLLGVEPAPLPEAPTAPRVGFCRPYFRDRLEPATQKLLEDGARRLARAG
                     AKVEEVMLPAADYEGLEAAHRWISGFEMARNLAWEIENHWERISEKFREGRLKDGLAC
                     SYERYCEMRDLVERCRERLAPVMNRHDVLLAPAAAGEAPAGVHPIPHPYVYMIWTTMH
                     VPSITLPVFEGPNGMPIGAQLVAKRCDDRRLLAVARWAYRVLRDS"
     gene            2416..3585
                     /locus_tag="AC16CX_09735"
     CDS             2416..3585
                     /locus_tag="AC16CX_09735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3012830.1"
                     /translation="MASDRPTWIDHFPGNFMWSNATLVTKGMAPYGAVALGEIDEVCE
                     RLRARQGEPHAWWEEWGALAASLEKAAGAAEAQGNRMSAGNLLLRAGMYYFTAERFIV
                     PGPGKREMGRKAIDCQTKGILGRYPNVERVEVPYEGGTLPALFMKAPGAKGRAPTMVA
                     FDGLDNCKEMSVLFNGLEFAARGWHTLAIDGPGQGESLRLRDVKTRHDYEAAGSAAYD
                     WVAARPEADAKRVAVMGYSFGGYYAPRIVAFEKRYCACVAFGAMHWNMVEWVDGIRRA
                     VKTDAKTSAQSHFQVPWAFGARDLDQAVEMAKRFNLAGVAGRIECPFLVVHGENDRIV
                     PLDSAKKLFDAVCSKRKHLKIFTAAEGAAEHCQVDNRQLGVDYIADWLAENAFGG"
     gene            complement(3659..4633)
                     /locus_tag="AC16CX_09740"
     CDS             complement(3659..4633)
                     /locus_tag="AC16CX_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012831.1"
                     /translation="MTHSRSLSGLAVVLVAAFTSLTASAQGYPAKTIRYVVPFPPAGA
                     TDILARWVAEKISPALGQTVVVENRPGAAGGVGTDFVAKSAPDGYTILMATAAQSISE
                     TLYVKQPFSFARDLAAVALIARVPNVMEVHPSVPARTVKEFIALAKARPGQLNYASSG
                     AGTTLHMSGELFKLMTGTDIVHVPYKGSAPAITDLMAGNVSVMWDNLPASMPYIKAGR
                     LRAIAITSARRYPAFPDLPTVSESGVPGYEASAWFGVVVPAATQREVIARLNSEINRA
                     VNLPDMKERFAQQGAIPAPGSPEDFAAWIHAEIAKWGKVVKASGARVE"
     gene            4852..5154
                     /locus_tag="AC16CX_09745"
     CDS             4852..5154
                     /locus_tag="AC16CX_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012832.1"
                     /translation="MTDFAMAVWAVGIAAWLVFVVYVYWIARQVWKDQLLKCPETGAI
                     TLVGIEYASQRAGSAPALTVQRCGLWPARAGCSRGCLARYEEILPGFAINLKSLRP"
     gene            complement(5235..5645)
                     /locus_tag="AC16CX_09750"
     CDS             complement(5235..5645)
                     /locus_tag="AC16CX_09750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012833.1"
                     /translation="MKIKRIEHVGIVVKDTEASRRLWEGCFGIPLGGVEENAQRRLAL
                     YYIGESMIELIAGTTPDSKHARMVAAGKGGLSHICFEVENIDEALAELKGKGIPLLDP
                     VPRIGHAGCRIAFIDPAATENCLIELAELPEGHT"
     gene            complement(5649..6089)
                     /locus_tag="AC16CX_09755"
     CDS             complement(5649..6089)
                     /locus_tag="AC16CX_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase"
                     /protein_id="HIR3012834.1"
                     /translation="MTKPFAQRIAAMNAMYKLPAHDRPTLPADVADRLAKFKATLLDE
                     VHEIDEIVQMSENGGAAIDLTVAIADLLSDIIVYCRSEALKYGLPLEEVLDVIMDSNE
                     SKLGADGKPIYDANGKFLKGPNYWRPEPKLKELLKARMKDDLKA"
     gene            complement(6378..>7165)
                     /locus_tag="AC16CX_09760"
     CDS             complement(6378..>7165)
                     /locus_tag="AC16CX_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3012835.1"
                     /translation="HPGKGTHNAIALNQEDYIELLAIRDPAEHEAAGRAGSWGADLAT
                     FIAAGGGIRYVILQSDDLAADVAAMRGRGVDVGDAIEGRRRTPAGQELRWKTAVLGPA
                     NPLPIFFIQHLTPMAERRKPVPDAGNHPNGVRFLERAYIVTPDVESAAAIYAKVLGIP
                     QPRRYKGTVIMADMAVFQFGPAGLTVAQPYAPGPAADALERRGPGPFQALYRTTSMSA
                     AARWMQEHGLPPPVRGVRDTGEQAMLVPPAEACGAYIGFVGPE"
BASE COUNT         1174 a         2349 c         2405 g         1237 t
ORIGIN      
        1 gatcacccgc gcccacttct gggattcggc gcgcatgaac ttcgcgaatt cctcgggcgt
       61 gctcgccacc ggctcggcgc ctaccgccgc gagctgcttg cgcacgtcgg gctgcgcaag
      121 cgtcgccgcc agcgtctggt gcagcttgcg gatgatctct ttcggcgtgc ctgcgggcgc
      181 gactaccgcg tgccaggcgg aggcgtcgag ccccgccagg ccgacctccg tgcaggcggg
      241 cacgtcgggc agcagcggcg agcgtttctg cgccgtgatg acgaacgcct tgagccggcc
      301 ggccttcacg tgcggcagga acgccgggat ggtattgaac gcaagctgga tccggcccgc
      361 tgccatctcg ggcagcgcct gggcgacgcc cttgaacgga acgtgcacga tgttgacgtc
      421 ggcggtgagc ttgaagagct cgcccatgag gtgcagcccg gagccgacgc cggtggagcc
      481 gaaattgagc tggccgggcc gcgccttcgc gtaggcgacc agctcccggg cggtagtcac
      541 gggcagcgtg gtgctcccga acagcacgaa cggcaggctc gtcgcctgcg aaacgggcgc
      601 gaggtccttg aacaggttgt agccgagctt ctgataaaga ttggggccga tcgagagcgg
      661 gccgagattg ccgtagctga tggtatagcc gtcgggcgtc gctttggcgg tcatttcggt
      721 gccgatcgtg ccgcccgctc caccgcggtt gtcgatcacc atctgctggc cgagcgccgt
      781 ctgccactgc tgcgcgatca atcgcgccgt ggcgtcaatt gactcgcccg ccgggaacgg
      841 cacgatcacg cggatcggct tcgtcggata ggtttgtgcc gccgcgctgt gacacaaacc
      901 tatgaataac gcgacaacta ttgacttgat cggccgtatc attattcctc cctgtcgaat
      961 cgcgattcac gaatcacgca gcacccggta cgcccagcgc gccaccgcaa gcagccgtcg
     1021 atcatcgcag cgcttcgcga cgagctgggc gccgatcggc atgccgttcg gcccctcgaa
     1081 caccggcagc gtgatcgatg gcacgtgcat cgtggtccag atcatgtaca cgtacggatg
     1141 gggtatcggg tgcaccccgg ccggcgcctc gcccgcggcg gccggcgcga gcaggacatc
     1201 gtgccggttc ataaccggcg ccaggcgttc gcggcagcgc tcgaccaggt cgcgcatctc
     1261 gcagtagcgc tcgtagctgc aggcgaggcc gtccttcagg cgcccctcgc ggaatttctc
     1321 gctgattctc tcccagtggt tctcgatctc ccacgcgagg ttgcgcgcca tctcgaagcc
     1381 cgagatccag cggtgcgccg cttccagccc ctcgtaatcg gcggccggca gcatcacctc
     1441 ctccactttc gcgccggcgc gcgcgaggcg ccgcgcaccg tcctcgagca gcttctgcgt
     1501 cgccggctcg agccggtccc ggaagtacgg gcggcagaag ccgacgcgcg gcgccgtcgg
     1561 cgcttcgggc agcggcgcgg gctcgacccc gagcagcacg tcgcgataga gcgccacgtc
     1621 atcgatcgag cgcgcgagga tgcccagcgt gtcgagcgat cccgacgcct ccatgacccc
     1681 cgccgtgcgc aggtcgccgt aggtgggacg gtagccgaac acgccgcaga acgaggcggg
     1741 ccgcgttgtc gagccggtgg tctgcgtgcc gatcgcgagc ggcacgtgga aatcggcgac
     1801 cgcggcggcc gagccgctcg aggagccgcc cggcgtgcgg gagagatcga gcgggttgcg
     1861 cgtcgggccg ggcgtgatgt tggcgaactc ggtggtgacg gtcttgccca tcaacacgcc
     1921 gcccgccttg cgcgagaggg cgacacaggc cgcgtccttc gccggctggt agcccttgtg
     1981 aatcggcgtg ccatattcgg tcggcaggtc gcaggtatcg atgatgtcct tcacgccaaa
     2041 cggcacgccg tgcagcggcc cgcgtcgggc gccgcggtcg agtgcgcgcg cttgtgcgat
     2101 cacgtgatca gtgtcgaggt gcgcccatgc cagcacctgc ggctcgcgcg ccgcgatgcg
     2161 ctcgaggcag gcgtgcgcga ccgcctcgca gctcgccctg ccggacgcca cctccgcgac
     2221 gatctcggtc gcggtgaact cgttcagtgc ccgcgcgcgc tgtcttgctg cgaccggcat
     2281 ggagcctcaa tgatgaatga tgaacgaact gcagtagaca gccatcagta taagcgctca
     2341 gtgcgcatgg gttaagattg gccgccggtg agatttcgga ccacttccat attgtgatca
     2401 ccacgagata accgtatggc ctccgaccgc ccgacctgga tcgatcattt ccccggcaac
     2461 ttcatgtggt cgaacgcgac cctcgtcacc aagggcatgg cgccgtacgg tgcggtggcg
     2521 ctgggcgaga tcgacgaggt gtgcgaacgg ctgcgcgcgc gccagggcga gccgcacgcg
     2581 tggtgggagg aatggggcgc gctcgccgcg agcctggaaa aagcggccgg tgccgccgaa
     2641 gcgcagggta accggatgag tgcgggcaac ctgttgctgc gcgccggcat gtattacttc
     2701 accgccgagc gcttcatcgt gccgggaccc gggaagcgcg agatggggcg aaaggcgatc
     2761 gattgccaga caaagggcat cctgggacgc tatccgaacg tcgaaagagt cgaagtgccg
     2821 tacgagggcg ggacgctgcc cgccctcttc atgaaggcgc cgggcgccaa gggacgcgcg
     2881 ccgacgatgg tcgcgttcga cgggctcgac aactgcaagg agatgagcgt cttattcaac
     2941 ggtctcgagt tcgcggcgcg tggctggcac acgctcgcga tcgacggccc cggccagggc
     3001 gagtcgctgc gcctgcgcga cgtcaagacg cgccatgatt acgaggccgc cggcagcgcg
     3061 gcctacgatt gggtcgccgc gcgcccggag gcggacgcga aacgcgtcgc cgtcatgggc
     3121 tacagcttcg gcggctacta cgcgccgcgc atcgtcgcgt tcgagaagcg ctattgcgcc
     3181 tgcgtcgcat tcggcgccat gcactggaat atggtggaat gggtggacgg catccgccgc
     3241 gcggtgaaga ccgacgcgaa gaccagcgcg cagtcgcact tccaggtgcc gtgggcgttc
     3301 ggcgcaagag acctcgacca ggcggtcgaa atggcgaagc gcttcaatct cgccggcgtc
     3361 gccggccgca tcgagtgccc gttcctggtg gtgcacggcg agaacgaccg tatcgtgccg
     3421 ctcgattcgg caaagaagct ctttgatgcg gtctgctcga agcgcaagca cctcaagatc
     3481 ttcaccgcgg ccgagggcgc ggcggagcac tgccaggtgg acaaccggca gctcggggtg
     3541 gactacatcg ccgattggct cgccgagaac gccttcggcg ggtgatgagt gatgggagcg
     3601 ggcggcagcg ccggctctaa cacctgtcac cgatcgcgag tcgcgagggt gaagtccatt
     3661 actcgacgcg cgctcccgac gccttgacca cctttcccca cttggcgatc tcggcgtgga
     3721 tccaggcggc gaagtcctcc ggcgagccgg gcgcggggat cgcgccctgc tgcgcgaagc
     3781 gctccttcat gtcgggcagg ttgaccgcgc ggttgatctc gctgttcagc cgcgcaatga
     3841 cctcgcgctg cgtggcggcc ggcaccacca ccccgaacca cgcgctcgcc tcgtagcccg
     3901 gcacgccgga ctcggagacc gtcggcaggt cggggaacgc cgggtagcgc cgggcggagg
     3961 tgatcgcgat cgcgcgcagc cgtcccgcct tgatgtaggg catcgaggcc ggaaggttgt
     4021 cccacatcac cgacacgttg ccggccatga ggtcggtgat cgccggcgca ctgcccttgt
     4081 acggtacgtg cacgatgtcg gtgccggtca tcagcttgaa cagctcgccc gacatgtgca
     4141 atgtggtgcc ggcgcccgag gaagcgtagt tgagctggcc gggccgggct ttcgcgagcg
     4201 caatgaattc tttcacggtc cgcgccggca cggatggatg cacctccatc acgttcggca
     4261 cgcgcgcgat caacgccacc gcggcgaggt cgcgggcgaa cgagaacggc tgtttgacgt
     4321 agagcgtttc gctgatcgac tgcgccgccg tcgccatcag gatcgtgtag ccgtcgggcg
     4381 cgctcttcgc gacgaaatcg gtcccgactc cgccagccgc gccggggcgg ttctccacca
     4441 ccaccgtctg gccgagcgcg ggggaaatct tctcggccac ccagcgcgcg agtatgtcgg
     4501 tcgcgcccgc gggcgggaac ggcacgacgt agcgtatcgt tttcgccgga tagccctggg
     4561 ccgaggcggt gagcgatgtg aaagcggcca ccagcacgac cgccagcccg gataacgaac
     4621 gggaatgagt catggcgttg ctccttgatc tgaaggccgc cagcctagca cttttccgga
     4681 tcgtggacta ttggagccaa tcttcgggct cgcgcggcca cggaacagcc tcccgccaga
     4741 gcgataaccg ggcgtgccgc caatgtgatt tcttgatctg gctcaatacc tagcgccccc
     4801 tcccggccgc agaatgacaa cggtcactca gtgccctggg aaggaggtca catgacggac
     4861 tttgctatgg cagtttgggc cgtgggaatt gcggcctggc tggtgtttgt cgtgtacgtc
     4921 tattggatcg ccaggcaggt ctggaaagac cagctgttga aatgccccga aacgggggcg
     4981 atcaccctgg tcggcatcga gtacgcgtca cagcgcgcag gaagcgctcc cgcactcacg
     5041 gtccagcggt gcggactgtg gccggcaagg gcgggctgtt ctcgcggttg cctcgctcgc
     5101 tacgaagaaa tcctgcccgg attcgcgatc aacctcaagt cgctgcggcc gtagagacgg
     5161 ctctaaaagg caaggatgaa ggcagaagga taacctggtc ctgactcccg cgctgcgggg
     5221 gtcatctttc tccgtcaggt gtgcccttcg ggcagctccg cgagctcgat tagacaattc
     5281 tccgttgccg ccggatcgat gaaagcgatg cggcacccgg catggccgat gcgcggcacc
     5341 gggtcgagca gcggaatccc ctttcccttg agctcggcca gcgcctcgtc gatgttctcg
     5401 acctcgaagc agatgtggct gagcccgccc ttgcccgccg cgaccatgcg cgcgtgcttg
     5461 ctatctggag tcgtccccgc aatcagctcg atcatcgatt cgccgatgta gtagagggca
     5521 agccgccgct gggcgttttc ctccacgccg ccgagcggaa tgccgaagca gccttcccaa
     5581 agccgccggc tcgcctcagt gtcctttacc actatcccta catgctcaat ccgtttgatc
     5641 ttcatgactt atgcctttag gtcatccttc atccttgctt ttagaagctc cttgagcttc
     5701 ggctcgggcc tccagtaatt cggccccttg agaaacttgc cgttcgcgtc gtagatcggc
     5761 ttgccgtccg cgccgagctt gctctcgttg ctgtccatga tgacgtccag cacctcctcc
     5821 agcggcaggc cgtacttcaa cgcctcggac cggcagtaca cgatgatgtc gctcagcagg
     5881 tccgctatgg cgaccgtgag atcaatcgcc gcaccgccgt tttccgacat ctgcacgatc
     5941 tcgtcgatct cgtgcacttc gtcgagcaac gtcgccttga atttcgccag ccggtctgcg
     6001 acatccgcgg gcagcgtcgg ccggtcgtgg gcggggagct tgtacatcgc gttcatcgcc
     6061 gcgatgcgtt gcgcaaatgg tttggtcatt ttgggaaaag gtgacgatca ccggtgacga
     6121 atgagtcaag tataagtcaa gttatcagtc aaccgcccgg acgatcgccc tccggcggag
     6181 ctgtcagccg tcagtaagca gcagctgcaa actgcgaact gattgccgca atctgtccac
     6241 tgcaaactgc ctactgaaaa ctgcaaatcc gggtaatcga ttcacgactt acgccattgc
     6301 acaatggcgg tgatgaagtg atgcagtgac gattcgaaga cggtgacacg gtgacggagt
     6361 ggcgaagtga cgggccgcta ctccggcccg acgaagccga tgtaggcgcc gcaggcctcg
     6421 gcggggggca cgagcatggc ctgctcgcca gtgtcgcgca cgccgcgcac ggggggcggc
     6481 aggccgtgct cctgcatcca gcgcgcggcc gcgctcatgc tggtggtgcg gtacagtgcc
     6541 tggaacgggc cggggccgcg gcgctccagc gcgtcggcgg ctggccccgg cgcgtacggc
     6601 tgcgcgacgg tgagcccggc ggggccgaac tggaacaccg ccatgtccgc catgatcacc
     6661 gtgcccttgt acctgcgcgg ctggggtatg cccagcactt tggcgtagat cgccgcggca
     6721 ctctcgacat ccggcgtgac gatgtaagcg cgctcgagaa agcgcacgcc gttgggatga
     6781 ttgccggcat ccggcaccgg cttgcggcgc tccgccatcg gcgtcaggtg ctggatgaag
     6841 aatatcggca gcgggttcgc ggggccgagc accgcggttt tccaccgcaa ctcctgcccg
     6901 gcaggggtgc gccggcggcc ttcgatcgca tcgccaacgt ccacgccgcg gccgcgcatc
     6961 gcggcgacgt ccgccgcaag gtcatcgctc tgcaggatga cgtagcggat cccgccaccc
     7021 gctgcgatga aggtcgctag gtcggcgccc cagctaccgg cccttcccgc cgcctcgtgc
     7081 tcggccggat cgcggatcgc gagcagctcg atgtagtcct cctggttgag cgcgatggcg
     7141 ttatgggtgc ccttgccggg gtgaa
//
LOCUS       DBNGWZ010000338         1562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1801987, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000338 DBNGWZ010000000
VERSION     DBNGWZ010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1801987"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="AC16CX_09765"
     CDS             complement(<1..936)
                     /locus_tag="AC16CX_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012836.1"
                     /translation="MKKRSVTSATFACFLLAAGVTNAQNYPTGPMRIVVPFAPGGSTD
                     IMARTIAQQLTERFRQQVLVENRPGASGTIGTAYVVKSPPDGHTMLLVQVSFVSNPSL
                     FKDLPYNQARDLTPVTNLASGPLTLTVHPSLPAKTVKQLIALGKARPGEINYGSPGVG
                     SISRLACALFNMMAGIEMTHVPYKGSGAAIADMLSGQVQFYAPNLFLSLPHIRAGKLR
                     ALGVTTAQRSPIAPDLPTIAEGGLPGYEVSTWFGIFVPSATPRENVNRLHQALAQIVK
                     SGEMQQRLAKDGLTPVASTPEEYAREVSRDTEKYGK"
     gene            complement(1126..>1562)
                     /locus_tag="AC16CX_09770"
     CDS             complement(1126..>1562)
                     /locus_tag="AC16CX_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HIR3012837.1"
                     /translation="TGEAREVAKVYGALAREVERHGAPWQPPVALISGGETTVTVRGR
                     GRGGRNCEFLLALTVDLNGADGIHALACDTDGIDGTEDNAGAILAPDSLKRAGKKKLD
                     AVTLLADNDGYGFFSALGDLVVTGPTRTNVNDYRVILVIGDE"
BASE COUNT          240 a          484 c          541 g          297 t
ORIGIN      
        1 cttgccgtac ttctcagtgt cgcgggagac ctccctggcg tactcctcgg gcgtgctcgc
       61 gaccggcgtc aggccatcct tggcaagccg ctgctgcatc tcgcccgact tcacgatctg
      121 cgcgagcgcc tggtggagcc ggttgacgtt ctcgcgcggc gtcgcgctcg ggacgaagat
      181 gccgaaccag gtcgaaacct cgtagccggg caatccgccc tcggcgatgg tcgggaggtc
      241 gggcgcgatc ggcgagcgct gcgcggtcgt cactcccagc gcgcggagct tcccggcccg
      301 gatgtgcggc aggctgagga acaggttcgg cgcgtagaac tggacctggc cgctcagcat
      361 gtcggcgatg gcggctcccg accctttata cggaacatgg gtcatctcga tgccggccat
      421 catgttgaag agcgcgcaag caagccgtga aatcgagccg acgcccggcg aaccgtagtt
      481 gatctccccc gggcgcgcct tgcccagcgc gatcagctgc ttgacggtct tcgcgggcag
      541 cgagggatgc acggtgagcg tcagcggccc ggaggcgagg ttggtcacgg gcgtcaggtc
      601 acgcgcctgg ttgtagggca ggtccttgaa gaggctggga ttcgacacga agctgacctg
      661 caccagcaac atcgtgtgcc cgtccggagg ggatttcacg acgtaggcgg tgccgatcgt
      721 gccgcttgcg ccgggacggt tttccacgag gacctgctgc cggaagcgct cggtgagctg
      781 ctgcgcaatg gtgcgcgcca tgatgtcggt ggagccgccc ggggcgaacg gcacgacgat
      841 gcgcatgggt cccgtgggat agttctgcgc gttcgtgacg cctgccgcga gcagaaagca
      901 agcaaacgtg gcggacgtga ctgatctctt tttcaccgga acctcctcgg gtgggtgtga
      961 ctggcgcgta ttattgatta cctggcgcgc cccgcttctc gcgggatcat gcgtacgctc
     1021 ggcgccgggt gtcaagccgt ctgattcggg tgactagtga tgcgtgagag gtgatatgtg
     1081 ataggtattg aattcccgcg cgaagcgcgg gcctcttatc acttatcact cgtcacctat
     1141 cacgagtata acgcggtaat cgttgacgtt ggtgcgcgtg gggccggtga cgaccaggtc
     1201 gccgagtgcg gagaagaagc cgtagccgtc gttgtcggcg agcaaagtca ctgcgtcgag
     1261 ttttttcttt cccgcgcgct tgagcgagtc gggcgcgagg atcgcgccag cgttgtcttc
     1321 ggtgccgtcg atgccgtcgg tgtcgcaggc gagcgcgtga atgccgtctg cgccgttgag
     1381 atccaccgtg agcgctagca ggaattcgca gttgcgcccg ccgcgtcctc ggccccgcac
     1441 ggtgacggtg gtttcgccgc ccgagatcag cgcgaccggc ggctgccacg gcgcgccgtg
     1501 ccgttcgact tcccgcgcga gcgcgccata aaccttcgcc acctcgcgcg cttcacccgt
     1561 ca
//
LOCUS       DBNGWZ010000339         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2937436, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000339 DBNGWZ010000000
VERSION     DBNGWZ010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2937436"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16CX_09775"
     CDS             <1..399
                     /locus_tag="AC16CX_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012838.1"
                     /translation="PAMLGGQVRIGFGPIVPAIPHVKAGRLKALGVTGTKRSLAAPDI
                     PTIAEQGLTGFGAASWYGVFVPAKTPKPVVARLNKEFAAALAAPEVKERLLRDGVEPE
                     ASSPEKLAALVLAERKTWAAVIKAAGIRIE"
     gene            695..1717
                     /locus_tag="AC16CX_09780"
     CDS             695..1717
                     /locus_tag="AC16CX_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3012839.1"
                     /translation="MIRAAIIGLGRWGQNLVNSVQGKSEAIRFVAGATRTVAKAEAYA
                     KEKGFPLHDSYEKALADPKVDAVVLATPHTRHAEQISAAAKAGKHVFTEKPLALTAKS
                     AAAAVRACAKAGVTLAVGYNWRFQPALQEIRKMLDDGRLGKPLHLEGNFCGPSAYRFP
                     ANHWRQEREEGPAGGMTGRGVHVVDAFLYLARRIESVHARSARQVLDHGLDDTTSMLF
                     RFRSGVTGYLGTVIATAETWRMQVFGSKGWVEVGDVEHLTTWEMRACFVDPRNLRVHH
                     KPQVITFPAVSTERAELEHFARAAEARRLLAVAGGDEVHGVEIFEAILKSAKSGKTVR
                     VGVEKA"
     gene            complement(1750..2046)
                     /locus_tag="AC16CX_09785"
     CDS             complement(1750..2046)
                     /locus_tag="AC16CX_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR3012840.1"
                     /translation="MDAGARVRAYDPVAAENTRRSVPSAWIGSGKLELVANQYDAVAG
                     ADALALVAEWKPFRNPDFEVMKKLMRTPIIFDGRNQYDPGHVRGLGFDYFGIGR"
     gene            complement(2157..3395)
                     /locus_tag="AC16CX_09790"
     CDS             complement(2157..3395)
                     /locus_tag="AC16CX_09790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3012841.1"
                     /translation="MSKKITIIGAGIVGMATASYLRRDGHDVTVVTQHPPGEYCSSGN
                     AGMLNNAGCVPQPMPGVLWKVPGYLIDPLGPLVVRWPYFAKALPWMLRFLANANVRQA
                     ERASKALYSLIKDTVITYERLVLHAGCSDLVRRSGYLVAYDSERSYKDDALAWKMRRD
                     RGVATEVLDTAGIHKLVPQLAPNYVLGVHILDQGYVMNPERLVKSLAAQFQKDGGVIQ
                     QRKVLYMETGESGVRALVTDGSRIPVETLVVCAGVHSGELTAKLGEPVPIEAERGYHV
                     TYSDPHLHLPLPVHVSDAKVFVTPMEMGVRVAGQAEFSGIYAEPDYRRADVLETHMKR
                     MFPQLRTADGTRWMGRRPAMPDSLPVIGPSVKQKNVYYAFGHGHLGLCGGAPTGRIVA
                     DLVSGRRPAIDLSPFRVDRF"
     gene            complement(3441..3515)
                     /locus_tag="AC16CX_09795"
     tRNA            complement(3441..3515)
                     /locus_tag="AC16CX_09795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3481..3483),aa:Glu,seq:ctc)
BASE COUNT          648 a         1177 c         1160 g          603 t
ORIGIN      
        1 ccggcgatgc tgggcggcca ggtgagaatc ggcttcgggc cgatcgtccc cgccataccc
       61 cacgtcaagg cgggccggct caaggcgctc ggcgtgaccg ggacgaaacg gtccctggcg
      121 gcgccggaca tcccgaccat cgccgaacag ggactgacgg gattcggagc ggcctcgtgg
      181 tacggcgtat tcgtcccggc aaaaaccccg aagcccgtgg tcgcgcggct caacaaggaa
      241 ttcgccgccg cgctcgcggc gccggaagtg aaagaacgcc tcctgcgcga cggtgtcgag
      301 ccggaggcaa gctcgcccga gaagctcgct gccttggttc ttgccgagcg caagacgtgg
      361 gccgctgtca tcaaggcagc cggcatccgg atcgagtgat ccggaaagga aaagcgtgat
      421 tcgtgactcg tgattcgtct ctcaccaccc cgcccgctgc gcgtgcaccc ctcctctgta
      481 aggaggggaa aatactcaaa ctcccctcct tttcaagggt gaggcggggt ttcgaagaag
      541 gtgcctgcgc accttcgagc cgccgaacgc cagccgcctc tgtgcggttg gccgggcggg
      601 gtggtgtaaa acgccggggt ggttgctgct ctcggcattt ttttcaggtc gaatcatgaa
      661 tcacaattca cgaatcacgc ttttgtgaaa tgaaatgatc cgcgcggcga tcatcggcct
      721 cggccgctgg ggccagaatc tcgtcaacag cgtgcaggga aaaagcgaag cgatccgctt
      781 cgtcgcgggc gcgacgcgta ccgtcgccaa agccgaggcc tacgccaaag agaagggatt
      841 cccactccac gactcgtacg agaaggcgct cgccgatccg aaggtggacg ccgtggtgct
      901 cgccacgccg catactcgcc acgcggaaca aatcagcgcc gccgcgaaag cgggcaagca
      961 cgtcttcacc gagaaaccgc tcgcgctgac cgcgaaaagc gcggcggcag cggtgcgcgc
     1021 ctgcgcgaag gccggcgtca cgctcgcggt cggttacaac tggcgttttc agcccgccct
     1081 gcaggaaatc cggaagatgc tggacgacgg ccggctcggc aagcccctcc atctggaggg
     1141 caatttctgc ggccccagcg cctatcgctt cccggcgaat cactggcgcc aggaacgcga
     1201 agaagggccc gccggcggca tgaccgggcg cggcgtgcac gtggtggacg ccttcctgta
     1261 tctcgccagg cgcatcgaat cggtgcacgc acggagcgcg cgccaggtgc tcgaccacgg
     1321 gctcgacgac acgacctcga tgctattccg cttcaggagc ggcgtgaccg gctatctcgg
     1381 gaccgtgatc gccaccgcgg aaacgtggcg catgcaggtg ttcggatcga aaggctgggt
     1441 ggaagtgggc gacgtcgagc acctgacgac gtgggagatg agggcttgct tcgtggaccc
     1501 gcggaacctg cgcgtgcacc acaagccgca ggttatcacc ttccccgccg tgagcaccga
     1561 gcgcgccgag ctcgagcact tcgcgcgcgc ggcggaagcg cgtcgcctgc tcgccgtcgc
     1621 cggcggcgat gaagttcacg gagtggagat attcgaggcg attctcaagt cagccaagtc
     1681 tggcaagact gtacgtgttg gtgttgagaa agcatgacat ggtgatgcgt gacatgtcac
     1741 gcttatcaat catctcccga tcccgaagta atcaaacccc aggccgcgga cgtgaccggg
     1801 atcgtactga ttgcggccgt caaagatgat cggcgttcgc atcagtttct tcatgacctc
     1861 gaagtcgggg ttgcggaacg gcttccattc cgcgaccagc gcgagcgcat ccgcgccggc
     1921 cacggcgtcg tattggttcg ccaccagctc cagctttccg gatccgatcc atgccgatgg
     1981 cacgctacgc cgggtgtttt cggccgcgac cgggtcgtaa gccctgaccc gcgccccggc
     2041 atccatcagc cgctcgtggc tgcgggtgaa cagcgcgtaa agcgcgcgca gctgttcgac
     2101 ggcatgctcg gacgatgatg ctcagcttca tgtcttgacc cggaaatccg ttagcttcag
     2161 aaccggtcga cgcggaacgg cgacaagtca atcgcggggc gtcgcccgga aaccaggtcg
     2221 gcgacgatgc gtcccgtcgg cgccccgccg cagagcccga ggtgcccgtg gccgaaggcg
     2281 taatagacgt ttttctgctt gaccgacggc ccgatcaccg gcagcgagtc gggcatggcc
     2341 gggcgccggc ccatccagcg cgtgccgtcc gcggtgcgta actgcgggaa catgcgcttc
     2401 atgtgggtct ccagcacgtc cgcgcgccgg taatccggct cggcgtagat gccggagaat
     2461 tccgcctgcc cggcgacgcg cacgcccatc tccatcggcg tcacgaacac cttggcgtcg
     2521 gacacgtgca ccggcagcgg caggtgcagg tgcggatcgg agtacgtgac gtgatagccg
     2581 cgctcggcct cgatcggcac cggctcgccg agcttcgccg tgagttctcc cgaatgcacg
     2641 ccggcgcaga ccacgagcgt ctccaccgga atcctgctgc cgtcggtgac gagcgcccgc
     2701 acgccgctct cgcccgtttc catgtacagc accttgcgtt gctggatgac gccgccgtcc
     2761 ttctggaact gcgcggcgag cgacttcacg agccgctccg gattcatgac atagccctgg
     2821 tcgagaatat ggacgccgag cacgtaattc ggcgcgagct gcggcaccag tttgtggatg
     2881 ccggccgtgt ccaaaacttc cgtcgccacg ccgcggtcgc gccgcatttt ccaggcgagc
     2941 gcatcgtcct tatagctccg ctcgctgtcg taggccacca ggtagccgct gcggcgcacc
     3001 aggtcgctgc agccggcatg cagcaccagc cgctcgtagg tgatcaccgt gtccttgatc
     3061 agcgaataga gcgccttcga ggcgcgctct gcctgcctca cgttcgcgtt ggcgagaaag
     3121 cgcagcatcc acggcagcgc cttggcgaag tacggccagc gcaccaccag cggccccagc
     3181 ggatcgatga gatagcctgg caccttccac agcacgcccg gcatgggctg cggcacgcag
     3241 ccggcgttgt tgagcatgcc ggcgttaccg gacgagcagt actcgccggg cggatgctgg
     3301 gtgacgaccg tcacgtcgtg cccgtcgcgg cgcaggtacg acgcagtcgc catgccgacg
     3361 atccccgcgc ctatgatggt gattttcttg ctcacggggt gatgctcagg gaggagcggg
     3421 aagtgttgga tcgggaaaaa tggtgggtgg tactgggttc gaaccagtga cccctgccgt
     3481 gagaagacag tgctctaccg ctgagctaac cacccacagc gaggcacgat tttatcacgg
     3541 ctcaatacgc accaccccgt caccttcggt gccactcctc ctcagaaa
//
LOCUS       DBNGWZ010000340         1971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2432757, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000340 DBNGWZ010000000
VERSION     DBNGWZ010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2432757"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC16CX_09800"
     CDS             <1..382
                     /locus_tag="AC16CX_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012842.1"
                     /translation="GTFLGTEGQAYRWMRRYGVIFYFGFTYLCMLITTGELWWLARAG
                     MITLPARFDRVLVVLCAVTLVIGLAQPFVPPFLDSEELKNRLENILEWYAALAFTLFF
                     LALAWLWRRARLTVRLESHGSLRR"
     gene            complement(371..1552)
                     /locus_tag="AC16CX_09805"
     CDS             complement(371..1552)
                     /locus_tag="AC16CX_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3012843.1"
                     /translation="MLGFVSLFMDVSSELIHSLLPVFMVTGLGATAFMVGVVEGIAES
                     TALIVKVFSGTLSDWLGKRKGLTVLGYGLGAVSKPVFALALSVHWVLAARFMDRIGKG
                     IRGAPRDALIADLTPPGIRGAAYGLRQSLDTVGAFLGPLLAIGLMLLWAGDFRTVFWF
                     AVIPAVLSVLILIFGVHEPASAREGRKAVSPIRWQTLGELGGAYWWVVVAGGVFTLAR
                     FSEAFLILRAQQLGLADTFAPLVLVVMNVVFAASAYPLGRLADRASHNTLLGAGLAVL
                     IVSDLVLALATGLAAVAAGVALWGLHMGMTQGLLATMVAGTAPPRLRGTAFGFFNLVS
                     GIAMLGASLLAGLLWDRLGAATTFYAGAVFSALALVLLARRRKRGAGSAPAKSGTASA
                     S"
     gene            complement(1600..>1971)
                     /locus_tag="AC16CX_09810"
     CDS             complement(1600..>1971)
                     /locus_tag="AC16CX_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3012844.1"
                     /translation="CQVTMFQGAARGMGASLAHGVSESRSADGWIVALADMPRIAPDT
                     IKRIIAGVEEGAQIVAPVYKGERGHPVGFGAQLRDELLALDGDQGARAIVERHRDVLR
                     LIECDDPGVLLDIDRKSDLPA"
BASE COUNT          361 a          681 c          625 g          304 t
ORIGIN      
        1 cggcaccttc ctcggcaccg agggacaggc ctaccgctgg atgcgccgct acggcgtgat
       61 cttctacttc ggcttcactt acctctgcat gctgatcacg accggggaat tgtggtggct
      121 cgcgcgcgcg ggcatgatca ccttgcctgc gcggttcgac cgcgtgctgg tcgtgctatg
      181 cgcggtcacg ctcgttatcg ggctcgctca gccgttcgtg ccgccctttc tggacagcga
      241 ggaactcaag aaccggctgg agaacatcct cgaatggtat gccgcgctcg cgttcacgtt
      301 gttcttcctt gcgctggcgt ggctgtggcg acgcgcgcgc ctgacagtgc gcctggaaag
      361 ccacgggtct ttacgacgct gaagcggttc cggatttcgc tggcgccgaa ccagcgccgc
      421 gcttgcgcct cctcgcgagg agcaccagag ccagcgcgga gaacacggcg ccggcgtaga
      481 acgtcgtggc cgcgccgagc cggtcccaga gcaggccagc aagcaggctt gcgccgagca
      541 tcgcgatgcc gctcaccagg ttaaagaaac cgaaggccgt gccacgcaag cggggcggcg
      601 cggtgcccgc caccatcgtc gccagcaggc cttgggtcat ccccatgtgc agcccccaca
      661 gggccacgcc ggccgcgacg gcggcaagcc cggttgccag cgccagcacc agatcggaaa
      721 cgatcaacac ggcgagcccc gctccgagca aggtgttgtg gctcgctcgg tcggcaagcc
      781 ggccgagcgg ataagcggag gcggcgaaga cgacgttcat gactaccagg accaacgggg
      841 caaatgtgtc ggcgagaccg agttgttgag ccctgagaat gaggaaggct tcgctgaagc
      901 gggcgagcgt gaacaccccg cccgcgacga cgacccacca gtaggcgccg cccagctccc
      961 cgagtgtctg ccagcgaatc ggggaaaccg ctttgcgccc ctccctcgct gacgcgggct
     1021 catgcacgcc gaaaatcagg atgaggacgg acaacaccgc cggtatcacc gcgaaccaga
     1081 ataccgtccg aaagtctcct gcccacagca acatcaggcc gatggccaga agcggcccaa
     1141 gaaatgctcc gaccgtgtcc agggattgcc tcaagccgta tgcggcgccg cggatcccgg
     1201 gcggggtcag gtcggcaatc agcgcgtcgc gcggcgcacc gcgtattccc ttgccgatcc
     1261 ggtccatgaa ccgcgcggcc agcacccaat gcacggacag cgcgagcgcg aataccggct
     1321 ttgacacggc gcccaggcca tagcccagca cggtcaagcc cttgcgcttc ccgagccagt
     1381 cgctgagggt ccccgaaaat actttcacga tcagggcggt ggactcggcg atcccctcga
     1441 ccacgccgac catgaacgcg gtagccccga gcccggtaac catgaagacg ggtaacagac
     1501 tgtggatcaa ctcggaggag acgtccatga acaggctgac gaagcccagc atccagacgc
     1561 cgcgcgggat tttcgtgggc gggatcaatt ggagcccgtt caagccggga gatcgctctt
     1621 gcggtcgatg tcgaggagca cgcccggatc gtcgcactcg atcaacctga gcacgtcgcg
     1681 gtgccgttcc acgatcgcgc gcgccccctg gtcaccgtcg agcgccagca gttcgtcgcg
     1741 aagctgtgcg ccgaagccga cggggtgacc gcgttcaccc ttgtaaaccg gtgcgacgat
     1801 ctgggcgccc tcttccaccc cggcgatgat cctcttgatc gtatccggcg caatgcgcgg
     1861 catgtccgcg agcgcgacta tccagccgtc cgcgcttcgt gattcgctta cgccatgcgc
     1921 caggctcgcc cccatcccgc gcgccgcgcc ctggaacatc gtgacctggc a
//
LOCUS       DBNGWZ010000341         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1551491, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000341 DBNGWZ010000000
VERSION     DBNGWZ010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1551491"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1336)
                     /gene="gcvPB"
                     /locus_tag="AC16CX_09815"
     CDS             complement(<1..1336)
                     /gene="gcvPB"
                     /locus_tag="AC16CX_09815"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3012845.1"
                     /translation="MLIFERSRRGRRSAAPAPAEAGDSAGIPERFLRTKPSLLPEASE
                     LDVVRHYTSLSQKNYSIDTHFYPLGSCTMKYNPRACNALAMLPSFLARHPSAPDDTGQ
                     GFLACLFELQEMLKAVTGMTQVSLAPMAGAQGEFAGVAMIRAYHDSRGDTARDEILVP
                     DAAHGTNPASAVMCGYAAREIATDANGNVDATALRNALGPRTAGLMLTNPSTLGVFER
                     HILEIGNAVHEAGGLLYYDGANLNAILGRVKPDDMGFDVIHINLHKTFSTPHGGGGPG
                     AGPVGVNAKLAPFLPLPVVGMAGDEYRLLDERDIPLSIGRLGAHLGNAGVLLRAYVYM
                     RMLGAEGMRRVADFATLNANYLMAELRRTGFEIAFPRRRASNEFIVTLKALKDETGVA
                     AMDVAKRLLDKGFHAPTAYFPLLVPECLLIEPTETESKQTLDAFVAAMKEILH"
     gene            complement(1340..>2741)
                     /gene="gcvPA"
                     /locus_tag="AC16CX_09820"
     CDS             complement(1340..>2741)
                     /gene="gcvPA"
                     /locus_tag="AC16CX_09820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR3012846.1"
                     /translation="MPFIPHTETDVREMLAAIGVASIEDLFEEIPRELRGAAFSGLPP
                     ALNEMEIARLMHERAGRDGTWLNFIGAGAYEHHIPAAVWQIATRGEFYSAYTPYQAEA
                     SQGTLQVLYEYQTMMASLTAHEVSNASLYDGASALAEAVLMAVRAHKASKTVLLPLTV
                     HPIWRKVVRTIVRNQGIELVEVPYYPDAGHTLPDALASLTGRDIAALVIPQPNFFGVL
                     EPVDELTDWAHANAALAVAAVNPTALALLKPPGEWGARGADIAVGDGQPLGAPLGSGG
                     PYFGFMTCRKDLVRQMPGRIVGATVDAEGRPGYVLTLQAREQHIRRSKATSNICTNQG
                     LMVTAATLYLALLGPAGLAGVARACHANTSALASRLTAIEGVARVFSGAFFHETVIRL
                     PVPVAPVLSALKAQGILGGRNLARDYPELGHALLVCATETRSEQDVALYAEHMARIIG
                     KRFRPAPCAVRPVNGT"
BASE COUNT          424 a          917 c          947 g          453 t
ORIGIN      
        1 cgtgcaggat ctccttcatc gcggcgacga aagcatcgag cgtctgtttc gactcggtct
       61 cggtcggctc gatcagcagg cattcgggca cgagcagcgg gaagtacgcg gtcggggcat
      121 ggaagccttt gtcgagcaga cgcttggcga cgtccatcgc ggcgacgccg gtttcgtcct
      181 tgagcgcttt cagggtgacg atgaattcgt tgctcgcccg ccgccgcgga aacgcgatct
      241 cgaatccggt tcttcgcaac tccgccatga ggtagttggc gttgagcgtc gcgaaatccg
      301 ccacccgccg catgccctcc gcacccagca ttcgcatgta gacgtaggcg cgcagcagca
      361 cgcccgcgtt gccgagatgc gcgcccaggc gcccgatcga gagcggaata tcgcgctcgt
      421 cgagcagccg atattcgtcc ccggccatcc ccaccaccgg cagcggcagg aacggcgcaa
      481 gcttcgcgtt gacgccgacc gggccggcgc cgggcccgcc gccgccgtgc ggcgtcgaga
      541 acgtcttgtg caggttgatg tggatgacgt cgaagcccat gtcgtcgggc ttgaccctgc
      601 cgagtatggc attgaggttc gccccgtcgt aatagagcag gccgcccgcc tcatgcacgg
      661 cgtttccgat ctccaggatg tggcgctcga acacgcccag cgtggacggg ttggtgagca
      721 tgagccccgc ggtacgcggg ccgagggcgt tcctcaacgc cgtcgcgtcc acgttcccgt
      781 tggcgtcggt ggcgatctcc cgcgcagcat agccgcacat cacggcggaa gcggggttgg
      841 tgccgtgggc ggcgtcgggc accaggatct cgtcgcgcgc cgtgtcaccc cgcgagtcgt
      901 ggtaggcccg gatcatcgcc acgccggcga actcgccctg cgcgcccgcc atcggcgcga
      961 ggctcacctg cgtcatgccg gtcacggcct tcagcatttc ctgcagctcg aacaggcagg
     1021 cgaggaagcc ctggccggta tcgtcgggcg ctgagggatg ccgcgcgagg aaggacggca
     1081 gcatggcgag cgcgttgcac gcgcgcgggt tgtacttcat ggtgcacgag ccgagcggat
     1141 agaagtgcgt gtcgattgaa tagttcttct gcgacaggct cgtgtaatgg cgcacgacgt
     1201 cgagctcgga cgcttccggc agcagcgacg gtttcgtgcg caggaatcgc tccgggatgc
     1261 cggctgaatc accggcttcg gcgggcgccg gtgccgcgct gcgcctgccc cgcctggagc
     1321 gctcgaagat cagcattgtt caggttccgt tgacgggtct gacggcacag ggcgccgggc
     1381 ggaaacgctt gccgatgatg cgcgccatgt gctccgcgta gagcgccacg tcctgctcgc
     1441 tccgggtctc cgtggcgcac accagcagcg cgtgcccgag ctcgggataa tcccgcgcca
     1501 gattccggcc gcccagtatg ccctgcgcct tgagcgcgct caacacgggc gcgaccggca
     1561 ccgggagccg gatcaccgtt tcgtggaaga acgcgccgct gaagacgcgc gcaacgcctt
     1621 cgatggcggt gaggcgggag gcgagcgcgg atgtgttggc atggcatgcg cgcgcgacgc
     1681 cggcgagccc tgccggcccg aggagcgcga gatagagcgt cgcggccgtg accatgagcc
     1741 cctggttggt gcagatgttg gatgtcgcct tggagcggcg gatgtgctgc tcgcgcgcct
     1801 gcagcgtgag cacgtagccg ggcctgccct cggcatccac cgtcgcgccg acgatgcgcc
     1861 ctggcatctg gcgcaccaga tccttacggc acgtcatgaa gccgaagtac ggccccccgc
     1921 tcccgagcgg cgcgccgagc ggctggccgt cgcccaccgc gatgtcggcg ccgcgtgccc
     1981 cccattcccc cggcggcttc agcagcgcga gcgcagtcgg gttgactgcg gcaaccgcga
     2041 gcgctgcatt ggcatgcgcc cagtcggtca actcgtccac cggctcgagc acgccgaaga
     2101 aattgggctg tggaatcacc agcgcggcga tatcccggcc cgtgagggac gcgagcgcgt
     2161 ccggcagcgt gtgtcctgcg tcggggtagt acgggacctc gacgagctca atgccctggt
     2221 tgcgcacgat ggtccgcacc accttgcgcc agatcgggtg cacggtgagc ggaagcagca
     2281 ccgttttcga tgccttgtgc gcgcgcaccg ccatcagcac cgcttccgcg agtgcggacg
     2341 ccccgtcgta gagactggcg ttcgaaacct cgtgcgcggt cagtgaagcc atcatggtct
     2401 gatactcgta gagcacctgc agggttccct ggctcgcttc cgcctggtac ggggtatacg
     2461 ccgaataaaa ctcgccgcgc gtggcgatct gccacaccgc cgccgggata tggtgctcgt
     2521 aggcgccggc gccgatgaag ttgagccagg tgccgtcgcg ccccgcgcgc tcgtgcatga
     2581 gcctcgcgat ctccatttcg ttgagcgcgg gggggaggcc ggagaacgcg gcgccgcgca
     2641 actcgcgcgg gatctcctcg aacagatcct cgatcgaggc aaccccgatc gccgcgagca
     2701 tctcgcgaac gtcagtctcg gtgtgtggga tgaacggcat a
//
LOCUS       DBNGWZ010000342         6435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1927632, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000342 DBNGWZ010000000
VERSION     DBNGWZ010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1927632"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16CX_09825"
     CDS             complement(<1..434)
                     /locus_tag="AC16CX_09825"
                     /inference="COORDINATES: protein motif:HMM:NF016246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3012847.1"
                     /translation="MIYTIGHGNRSIEEFLGLLREACIECLVDVRAYPASRRHPQFAR
                     EALERSLAEAGVRYMWEGKDLGGRRKLAKDSPNIALRSPGFRAYADHMATEEFREGIG
                     WLIELGRSTRAAVMCAERLPWECHRNLISDSLVARGIPVTHV"
     gene            complement(618..1346)
                     /locus_tag="AC16CX_09830"
     CDS             complement(618..1346)
                     /locus_tag="AC16CX_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3012848.1"
                     /translation="MIFLFTDFGSRDLYVGQVKAVLHQRAPEARVVDLLHEAPAFNVK
                     ASAHLLAALAARIPAGSITVAVVDPGVGGARKPIAVLADDRWFVGPDNGLISVLAARA
                     KKEEAYAIGWKPQSLAASFHGRDLFAPVAGMLARGDRRGARLEKKARLAANFGGDDLA
                     EIIYMDHYGNAMTGLRAGGLSRKTRLMVGDRWIARARVFSEVPRGEAFWYENSIGLAE
                     IAANSASVASKLGLAVGTLIGIST"
     gene            complement(1732..2091)
                     /gene="rplQ"
                     /locus_tag="AC16CX_09835"
                     /pseudo
     CDS             complement(1732..2091)
                     /gene="rplQ"
                     /locus_tag="AC16CX_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(2280..3266)
                     /locus_tag="AC16CX_09840"
     CDS             complement(2280..3266)
                     /locus_tag="AC16CX_09840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3012849.1"
                     /translation="MVSTAFLKPRIIDVQNISPYHAKLTMEPFERGYGHTLGNALRRT
                     LLSSMPGYAPTEVKITGVLHEYSTLDGVQEDVVDLLLNLKGIVLKLHNREEVLLTLRK
                     SGEGAVTAGDIEPVHDVEIVNPDHVIAHLAAGGKLDMQIKVEQGRGYIAATTRRTSVE
                     EGRNVGSILLDASFSPVRRVSYAVEAARVEQRTDLDKLVIDVETNGAIEPEEAVRYAA
                     RILVEQLSVFADLKGLPTHIEEKKATQIDPILMRPVDDLELTVRSANCLKAENIYYIG
                     DLIQRTETELLKTPNLGRKSLNEIKEVLATRGLTLGMKLENWPPAGLEKLTT"
     gene            complement(3287..3913)
                     /gene="rpsD"
                     /locus_tag="AC16CX_09845"
     CDS             complement(3287..3913)
                     /gene="rpsD"
                     /locus_tag="AC16CX_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR3012850.1"
                     /translation="MARNLDAKCRQCRREGEKLFLKGEKCFTDKCAIERRNYPPGQHG
                     QKHSRLSGYGVQLREKQKVRRIYGLLERQFRKIYHAAEQMKGITGESLLQLLESRLDT
                     VVYRMGFGSSRTEARQIVRHNGILVNGKRVNMPSFQCRPGDVIEVAEKAKSQLRIKGA
                     AEAAEGRGFPEWLEVDVKGLKGKFKARPQRTELPSTINESLVVELYSK"
     gene            complement(3927..4316)
                     /gene="rpsK"
                     /locus_tag="AC16CX_09850"
     CDS             complement(3927..4316)
                     /gene="rpsK"
                     /locus_tag="AC16CX_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR3012851.1"
                     /translation="MAKASTRVRKKAKKNVAEGIAHIHASFNNTIVTITDRQGNTLAW
                     ATCGSNGFKGSRKSTPFAAQVAADRAGRLAQECGVKNLEVHIKGPGPGRESAVRALNA
                     VGFKITQISDVTPVPHNGCRPPKKRRV"
     gene            complement(4336..4713)
                     /gene="rpsM"
                     /locus_tag="AC16CX_09855"
     CDS             complement(4336..4713)
                     /gene="rpsM"
                     /locus_tag="AC16CX_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR3012852.1"
                     /translation="MARIGGVNIPNHQHAEIALTAIFGIGRARARLICDAAGVGRSVK
                     IKDLSDTQMDKLREQVSKIPVEGDLRREVSMSIKRLIDLGTWRGKRHRLGLPVRGQRT
                     RTNARTRKGPRKAAVKIIRAVVA"
     gene            complement(4792..4905)
                     /gene="rpmJ"
                     /locus_tag="AC16CX_09860"
     CDS             complement(4792..4905)
                     /gene="rpmJ"
                     /locus_tag="AC16CX_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR3012853.1"
                     /translation="MKVLASVKKICRHCKIVRRKGVVRVICKNPRHKQRQG"
     gene            complement(5032..5250)
                     /gene="infA"
                     /locus_tag="AC16CX_09865"
     CDS             complement(5032..5250)
                     /gene="infA"
                     /locus_tag="AC16CX_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3012854.1"
                     /translation="MAKEEMIQMQGEIVETLPNATFRVKLENGHMVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLTRGRIVFRAK"
     gene            complement(5250..>6435)
                     /gene="secY"
                     /locus_tag="AC16CX_09870"
     CDS             complement(5250..>6435)
                     /gene="secY"
                     /locus_tag="AC16CX_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3012855.1"
                     /translation="RAGGTLFGLADLFSGGALRRLSIFALNIMPYISASIIFQLLAVV
                     VPQLEKLQKEGEQGRKRINQYTRYATVLLVAIQAVGIAFGLEGMRSPGGAAVILDPGW
                     GFRLMTTITLTAGTIFIMWLGEQITERGIGNGISLIIFAGIAAGLPHAVAGTLELVRT
                     GAFSILIALVIFTLVLVVTAFVCFVERGQRRVLVNYARRQVGNKIYGGQSSHLPLKLN
                     MSGVIPPIFASSIILFPATLVGWFGSSEGFTWLKNIAGTLSPGQPIYVILYSSAIIFF
                     CFFYTALVFNPKETADNLKKSGAFVPGIRPGDQTARHIERIMMRLTLTGAIYVTLVCL
                     LPEFLILKWNVPFYFGGTSLLIIVVVTMDFMAQVQAYMMSHQYESLLKKASFKGGMFP
                     NR"
BASE COUNT         1049 a         2082 c         2062 g         1242 t
ORIGIN      
        1 atgacgtggg tgaccggaat tccgcgcgcc accaggctgt cggagatcag gttgcggtgg
       61 cattcccacg gcagccgctc ggcgcacatg acggcggcgc gcgtcgatcg ccccagctcg
      121 atcagccacc cgatgccttc acggaattcc tccgtcgcca tgtggtcggc gtaggcgcga
      181 aagccggggc tcctcagcgc gatgttaggc gagtccttgg cgagcttgcg ccggccgccc
      241 aggtctttgc cttcccacat gtagcggacg ccggcttcgg cgagcgagcg ttccaatgcc
      301 tcgcgcgcga actgcggatg gcggcgcgag gccgggtaag cgcgcacgtc caccaggcac
      361 tcgatgcagg cctccctgag gagtccgagg aactcctcga tggagcggtt gccgtggccg
      421 atggtgtaga tcatgagacc cgtgattcgt gagtcgtgag tcgtgattcg agacagccgg
      481 gagatcgtga gatcgggaaa tcgggagatc gagaagccgg gtggtcggaa gatcgggaga
      541 tcgtgagagg gacttatctc gcgctcacgc cctcccgccc taacgccctc acggttagca
      601 tcgttcgcca cccgccatca ggtcgaaatg ccgatgagtg ttccgaccgc gaggcccagc
      661 ttgctggcga cgctggcgga attcgcggcg atctccgcga ggccgatgct gttctcgtac
      721 cagaatgcct cgccccgcgg cacctcggaa aagacccgtg cgcgggcgat ccagcggtcc
      781 ccgaccatca accgcgtctt gcgcgaaagc ccgcctgcgc gcagccccgt catcgcattg
      841 ccgtagtgat ccatgtagat gatttccgcg aggtcgtcgc ccccgaaatt tgccgcgagc
      901 ctcgcttttt tctccaatcg cgcgccgcgg cgatcgccgc gcgcgagcat ccccgccacc
      961 ggcgcgaaca gatcgcggcc gtgaaacgac gcggccaggg attgcggctt ccagccaatg
     1021 gcgtaggctt cctccttctt cgcgcgcgcg gcgaggacgg agatgaggcc gttgtctggc
     1081 ccgacgaacc agcggtcgtc agccagcacc gcaatcggtt tccgcgcgcc gccgacgccg
     1141 ggatccacca ccgcgaccgt gatgctcccc gccgggatcc gcgccgccag cgcggcgagg
     1201 agatgggcgc tggccttcac gttgaacgcc ggcgcctcgt gcaaaagatc caccacccgc
     1261 gcctcgggtg cgcgttggtg cagcaccgcc ttcacctgcc cgacgtagag gtcgcggctg
     1321 ccgaaatcgg tgaacagaaa aatcatgaat tagccgccaa gcttgccccg gacttgatcc
     1381 gggagtgcca agaacgccag gaaatccaaa agtcgaacga tttaacgaaa tcatcaaata
     1441 tcatacgcgc ctagccatta ttcgttgaag acaccgcgca gtgaacggcg attcgtatcg
     1501 tccgttcgac tgggcggagt taaaccggcg cgtgagcgtc cggatttaat ggcacctgga
     1561 agcgccgcgg cccgcagaaa agaaaaaagc cggctttcgg ccggctttga aaacgcagtg
     1621 tggtgcctat gcagccttgg cggccgcctc tttcttcgac cccttcttgg ccccctccct
     1681 gttgtccttt gtggtcttcg cttccttgcc ctgcgcgcct tccgcgccgg cctccggacg
     1741 gtccatcagc tccacgaacg ccatcggtgc gttgtcgccc ttacggaagc cggacttgag
     1801 gatgcggaga tagccgccat tgcgcttcgc gtagcgcggc cccagctcgt cgaacacctt
     1861 caccaccacg tcgcggtcgc gcaggcggtc gaaggcgagc cggcggttgg cgagcgaggg
     1921 cctcttgccg agcgtgatga tgggctcgac cacgcgccgc agctccttcg ccttgggcag
     1981 cgtggtctgg atcgcctcgt gccgcagcag cgagttcgcc atgttgcgga acatcgccag
     2041 gcgatggctg ctggtgcggt tgagcttgcg gagcccgtgt ctgtgacgca tcgtgagttc
     2101 ctcactgagg gatgagggat gaggggcgag ccgggaattc gacgaacgtg cctgcgcacg
     2161 ttctagccgg cgaacgcccg acgcgatgca acgcggcggg ccggggtcga agaccaggga
     2221 tgacccgaaa acccgggttt accttcatcc ttccgccttc atccttcatc cttgatttct
     2281 caagttgtca gcttctccag cccggctgga ggccaattct cgagtttcat tcccaaggtg
     2341 aggccgcgcg tggccagcac ttccttgatc tcgttcagcg acttgcgccc gaggttcggg
     2401 gtcttgagga gctcggtctc ggtgcgctgg atgaggtcgc cgatgtagta gatgttctcg
     2461 gccttgaggc agttcgccga gcgcacggta agctcgagat cgtccaccgg ccgcatcagg
     2521 atcgggtcga tctgcgtcgc cttcttctcc tcaatgtggg tgggcaggcc cttgagatcg
     2581 gcgaacaccg agagctgctc gaccaggatg cgcgcggcgt agcgcaccgc ttcctcgggt
     2641 tcgatcgcgc cgttggtctc gacgtcgatc accagcttgt cgaggtcggt gcgctgctcg
     2701 acgcgcgcag cctccacggc gtagctcacg cgccgcaccg ggctgaacga ggcgtccagc
     2761 aggatcgagc cgacgttgcg tccttcctcg acggaagtgc ggcgggtggt ggccgcgatg
     2821 tagccgcgcc cctgctcgac cttgatctgc atatcgagct tgccgccggc ggcgagatgc
     2881 gcgatgacgt ggtcgggatt gacgatttcc acgtcgtgca ccggctcgat gtcgcccgcg
     2941 gtgaccgcgc cctcgccgct cttcctgagt gtcaacagca cttcctcgcg gttgtggagc
     3001 ttcaacacga tgcccttgag gttcaacagg aggtcgacca cgtcctcctg cacgccgtcg
     3061 agcgtcgagt actcgtgcag cacacccgtg atcttgacct cggtcggcgc gtaacccggc
     3121 atcgaggaca ggagcgtcct gcgcagcgcg ttgcccaggg tgtgcccgta gccgcgctcg
     3181 aacggctcca tggtgagctt ggcgtggtac ggcgagatgt tctggacgtc gatgatgcgg
     3241 ggcttgagaa atgcagtgct aaccatggct aggatacctc tgatggttac ttcgagtaga
     3301 gctcgaccac gagcgattcg ttgatggtgg acgggagctc cgtgcgttgc ggccgcgcct
     3361 tgaacttgcc tttgaggccc ttcacgtcca cttccagcca ctccgggaag ccgcggcctt
     3421 cagccgcttc ggccgccccc ttgatgcgca gctggctctt ggccttttcg gcgacttcga
     3481 tcacgtcgcc cggacggcac tggaacgagg gcatgttgac gcgcttgccg ttcaccagga
     3541 tgccgttgtg gcgcacgatc tggcgcgcct cggtgcggga ggaaccgaag cccatgcggt
     3601 agaccaccgt gtcgagccgg ctctccagca gctgcagcag gctctcgccg gtgatgccct
     3661 tcatctgctc ggcggcgtgg tagatcttgc ggaactggcg ctcgagcagc ccgtagatgc
     3721 ggcgcacctt ctgcttctct cgcagctgca cgccgtagcc cgagagacgg ctgtgcttct
     3781 ggccgtgctg tcccggcgga taattgcggc gctcgatggc gcacttgtcg gtgaagcact
     3841 tctcgccctt gaggaagagc ttctcgcctt cccggcggca ttgcctgcac tttgcgtcaa
     3901 gatttctggc catattcgat gttctcctag acgcggcgct tcttcggcgg gcggcagccg
     3961 ttgtgcggca ccggcgtcac gtccgatatc tgcgtgatct tgaagcccac ggcgttcaac
     4021 gcccgcaccg cggactcgcg ccccggcccc ggccccttga tgtgcacttc caggttcttc
     4081 accccgcatt cctgcgcgag ccgcccggcg cggtcggccg cgacctgcgc ggcgaacggc
     4141 gtggacttgc gcgatccctt gaaaccgttc gagccgcagg tcgcccaggc aagcgtgttg
     4201 ccctggcggt cggtgatggt gacgatggtg ttgttgaagg acgcgtggat gtgggcgatg
     4261 ccctctgcca cgttcttctt cgccttcttg cgcacccggg tgctggcttt tgccatggtg
     4321 atgggtcctt gtcctttagg cgacgaccgc cctgatgatc ttcaccgccg ccttgcgcgg
     4381 gcccttgcgc gtgcgcgcgt tggtgcgggt gcgctggccg cgtaccggca gccccaggcg
     4441 gtggcgcttg ccgcgccagg tgccgagatc gatcaggcgc ttgatgctca tcgagacctc
     4501 gcggcggagg tccccttcga ccgggatctt cgagacctgc tcgcgcagct tgtccatctg
     4561 ggtgtccgag aggtccttga tcttcaccga gcggccgacg cccgccgcgt cgcagatcag
     4621 gcgcgcgcgc gcgcggccga tgccgaagat cgcggtcaac gcgatctcgg cgtgctgatg
     4681 gttgggaatg ttgactccgc cgatccgggc catgcctgtc ctcgtagcga aaagtttgcg
     4741 attctagcgt ctaacctgtt aattttcaat ctaaaacgag ccgcgcccgg tttacccctg
     4801 gcgctgcttg tgccgcgggt tcttgcagat cacccgcacc acgcccttgc ggcggacgat
     4861 cttgcagtgg cggcagattt tcttcaccga tgccagcact ttcatgtccg ttgtccttcg
     4921 ccaaaagtct cgacgcgaag ccgcaaagtc gcaaagaaca tcatctgact caacattcgc
     4981 cgcattgttc ctgtcttcct ttgcgtcttt gcgcctttgc gtcgtggtct tctacttcgc
     5041 gcggaacacg atccgcccgc gcgtcagatc gtagggcgtc agctccaccg tcaccttgtc
     5101 gcccggcagg atgcggatgt aatgcatgcg catcttgccc gagatgtgcc ccagcaccat
     5161 gtgcccgttc tcgagcttca cccggaaggt cgcgttgggc agggtctcca cgatctcgcc
     5221 ctgcatctgg atcatctctt ccttggccat cagcggttcg ggaacatccc ccccttgaaa
     5281 ctcgccttct tgagcaggct ctcgtactgg tgcgacatca tgtaggcctg cacctgcgcc
     5341 atgaagtcca tggtcaccac cacgataatg agcagcgagg tgccgccgaa atagaacggc
     5401 acgttccact tgaggatcag gaattcgggc agcaggcaca ccagcgtgac gtagatcgcg
     5461 ccggtgagcg tgagccgcat catgattctc tcgatgtggc gcgccgtctg gtcgcctgga
     5521 cggatgccgg ggacaaaggc accgctcttc ttcaggttgt cggcggtctc cttggggttg
     5581 aacaccagcg ccgtgtagaa gaagcagaag aagatgatcg ccgaggaata caggatcacg
     5641 tagatcggct gcccggggga gagcgtgccc gcgatgttct tgagccaggt gaacccctcg
     5701 ctcgagccga accagcccac cagggtggcc gggaacagaa tgatcgagga ggcgaagatc
     5761 ggcgggatca cccccgacat gttgagcttg agcggcaggt gcgagctctg gccgccgtag
     5821 atcttgttgc cgacctggcg cctggcgtag ttcacgagaa cccggcgctg gccgcgctcg
     5881 acgaaacaga cgaacgcggt caccaccagc acgagcgtga agatcacgag cgcgatcagt
     5941 atcgagaacg ccccggtgcg caccagctcg agcgtgccgg cgaccgcgtg cgggagcccc
     6001 gcggcgatgc ccgcgaagat gatgagggag atgccgttgc cgatgccgcg ctcggtgatc
     6061 tgctccccca gccacatgat gaagatggtg ccggccgtga gggtgatggt ggtcatgagc
     6121 cggaaccccc agccggggtc gaggatcacc gcggcgccac cggggctgcg catcccctcc
     6181 aagccgaagg cgatccccac ggcctggatg gcgaccagga gcaccgtggc gtagcgggtg
     6241 tactggttga tgcgcttgcg tccctgctcc ccctccttct ggagcttctc gagctggggg
     6301 accaccaccg ccaggagctg gaagatgatg gacgcactga tgtagggcat gatgttcagc
     6361 gcgaagatgc tcaggcgccg cagggctccg cccgaaaaca ggtccgccag gccgaagagg
     6421 gtcccgccgg cccgg
//
LOCUS       DBNGWZ010000343         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2937948, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000343 DBNGWZ010000000
VERSION     DBNGWZ010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2937948"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16CX_09875"
     CDS             <1..366
                     /locus_tag="AC16CX_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR3012856.1"
                     /translation="VALARLLGADETVVGGLYAKSLTAPIAMGVAERIGASPTLTAVF
                     TVTTGILGAALGRFVLDAVRCREWWQRGFAIGVASHGIGTSRAFSVHPEAGAYAGLAM
                     GLHGVIGAIVIPWAISALR"
     gene            complement(498..1205)
                     /locus_tag="AC16CX_09880"
     CDS             complement(498..1205)
                     /locus_tag="AC16CX_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012857.1"
                     /translation="MRNEPSRYQSALIVGAGQGLSASLARLFSREGMRVALASRNPDR
                     LAALCAETGARAFVCDVVNAGEVRRLFSEVESAIGEPDVVVYNASARMRGPLVELDPA
                     EVARTLTVTAFGGFLVAQQAAQRMLAKQHGAIFFTGASASVKGYPQSAPFAMGKFALR
                     GLAQSMAREFAPQGIHVAHFVIDGGIARPGRTPPSDMPDSLLEPDAIAANYLHVLRQP
                     RSAWTWEIELRPWVEKF"
     gene            complement(1195..1401)
                     /locus_tag="AC16CX_09885"
     CDS             complement(1195..1401)
                     /locus_tag="AC16CX_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012858.1"
                     /translation="MRQPMVALFIALLAGCGVDTATTAATGAAIKKQEMDAGKQTLEQ
                     TQQKLEQAAQQTQQRAEQAGGSEK"
     gene            complement(1460..2047)
                     /locus_tag="AC16CX_09890"
     CDS             complement(1460..2047)
                     /locus_tag="AC16CX_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3012859.1"
                     /translation="MIDTAEMLKVFEKHRGDAIVIPGRAGRHWVDISMQPNRDLPIGD
                     PAMGGHASFALGLALAQPREKVVVFDSEGDVLMGLGALATIAEQQPKNFYHFLLDNEC
                     YATTGGQPVPNAKKVSYDGLARACGYDRTHAFDSLERFAIEIEQILAGTGPVLVWMKI
                     RPEVENLPIKQRKQWRKRPPAQTLLDLRKELGVAD"
     gene            complement(2044..>2422)
                     /locus_tag="AC16CX_09895"
     CDS             complement(2044..>2422)
                     /locus_tag="AC16CX_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012860.1"
                     /translation="FSTAAGVWAGGGRPVVMIQNTGLMESGDSLRGWILGLNIPEVLL
                     VGYRGWTRHGVNTDSAAIYTERFLNAFGVNYYLVEGDADAPRISIAFEEAARTKRPVA
                     VLVADEYHGFNRQGERRKSKGGR"
BASE COUNT          355 a          827 c          801 g          439 t
ORIGIN      
        1 gtggcgctcg cgcggctgct gggcgcggac gaaacggtgg tcggcgggct ctacgccaag
       61 tcgctcacgg cgccgatcgc gatgggggtg gccgagcgca tcggcgcctc ccccacgctg
      121 acggcggtgt tcaccgtcac caccggcatc ctcggcgcgg cgctgggccg gttcgtgctc
      181 gacgcggtgc gctgccgcga atggtggcag cgcggctttg cgatcggcgt ggcctcgcac
      241 gggatcggca cctctcgcgc gttcagcgtg catcccgaag cgggggccta cgccgggctc
      301 gcgatggggc tgcacggcgt aatcggcgcg atcgtgatcc cgtgggcgat ctcggcgctg
      361 cgctaatgcc cgaaaggcgt gataggtgat gggtgatggg tcaagtaaat tgccggcagc
      421 gccggccctt cttatcactt atcacttatc acttatcact tatcacttat cacttatcac
      481 tcatcacttg ctactcctca gaacttctcc acccagggcc gtaactcgat ctcccacgtc
      541 caggcgctgc ggggctggcg cagcacgtga aggtagttcg ccgcaatggc gtcgggctcg
      601 agcagggagt cgggcatgtc cgacggcgga gtgcgtccgg gccgcgcgat gccgccgtcg
      661 atgacgaagt gcgcgacatg gatgccctgc ggcgcgaact cgcgcgccat gctctgggcg
      721 aggccgcgca gggcgaactt gcccatcgcg aacggcgccg actgcgggta gcccttgacg
      781 ctcgccgagg cgccggtgaa gaaaatcgcg ccgtgttgct tcgcgagcat gcgctgcgcg
      841 gcctgctgcg cgaccagaaa accgccgaag gcagtcaccg tcagcgtcct cgcgacctcg
      901 gccggatcga gctcgacgag cggcccgcgc atccgtgcgc tggcgttata cacgaccacg
      961 tccggctcgc cgatcgcgct ctccacctcg gaaaaaagcc gccgtacctc acccgcgttg
     1021 accacatcgc atacgaaggc gcgcgcgccg gtttccgcgc acagcgcggc aagccggtcc
     1081 gggttgcgcg aggcgagcgc gacgcgcatc ccttcgcgcg agaatagccg ggcgagggaa
     1141 gcgctgagcc cctgccccgc cccgacgatc agcgcgcttt ggtatcgtga tggttcattt
     1201 ctcactgccg ccggcctgct cggcgcgctg ctgcgtctgc tgcgccgcct gctcgagctt
     1261 ctgctgcgtc tgctccagcg tttgcttccc cgcatccatt tcctgcttct tgatcgcagc
     1321 gccggtcgcg gcggtggtgg cggtgtccac cccgcagccg gcaagcagag cgatgaagag
     1381 tgcaaccatc ggctgtctca tgggttctct ccttgaacgg cgtgaatcgt aagcgccttc
     1441 cccgtgacgc gtcgccgctt caatcggcga cgcccagctc tttcctgagg tccaggagcg
     1501 tctgggcggg agggcgcttg cgccactgct tgcgctgctt gatcgggagg ttctcgactt
     1561 ccggccggat cttcatccag acgagtaccg gccccgttcc ggccagtatc tgctcgatct
     1621 cgatcgcgaa gcgctccagg ctgtcgaagg cgtgggtccg gtcgtagccg catgcgcgcg
     1681 cgaggccgtc gtacgaaacc ttcttggcgt tgggcacggg ctgccctccg gtggtggcgt
     1741 agcactcgtt gtcgagcagg aagtgataaa agttcttcgg ctgctgctcc gcgatggtcg
     1801 cgagcgcccc gaggcccatc agcacgtcgc cttcggaatc gaacaccacc accttctcgc
     1861 gcggctgcgc cagcgcgagg cccagcgcga acgacgcgtg cccgcccatc gccgggtcgc
     1921 cgattggaag atcccggttg ggctgcatgc tgatgtcgac ccagtggcgg cccgcccgtc
     1981 ctgggatcac gatcgcatcg ccccggtgct tctcgaagac cttcagcatc tccgccgtat
     2041 cgatcatctt ccgcctttcg actttcgcct ttcgccttgg cggttgaatc cgtgatactc
     2101 gtccgcgacc agaacggcga ccggccgctt ggtgcgggcc gcctcctcga aggcgatcga
     2161 gatgcgcggt gcatcagcgt cgccctcgac caggtagtag ttgacgccga aggcgttgag
     2221 gaagcgctcg gtgtagatgg cggcgctgtc cgtgttgacg ccgtggcgcg tccagccgcg
     2281 gtagccgacc agcagcacct cggggatgtt gaggccgagg atccagccgc gcagcgagtc
     2341 acccgactcc atgagcccgg tgttctggat catgaccacg ggcctgccac cgcccgccca
     2401 cacgccggcg gcggtggaga ag
//
LOCUS       DBNGWZ010000344         3586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_416739, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000344 DBNGWZ010000000
VERSION     DBNGWZ010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_416739"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16CX_09900"
     CDS             complement(<1..283)
                     /locus_tag="AC16CX_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3012861.1"
                     /translation="MRAHLESHRFISQLTKTSFAFILAGGRGSRLAQLTSERAKPAVS
                     FGGKFRIIDFTLSNCVNSGVRRIGVVTQYKAHSLIRHIQRGWSFLDGRFD"
     gene            570..2735
                     /gene="glgB"
                     /locus_tag="AC16CX_09905"
     CDS             570..2735
                     /gene="glgB"
                     /locus_tag="AC16CX_09905"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3012862.1"
                     /translation="MQFTDERTERLLAATSHDPFACLGPQREGAGWVVRVFRPRAEAV
                     AMQGAAGAAAFARVHSAGIFEWRSPQPPPHPYRLLVTENGRARSEHDPYAFPPRPSAH
                     DLYLFSQGTNYQAYRILGARCETREGVAGVRFRVWAPNAERVSVVGDFNEWDGRVHPL
                     ATLGASGVWELFVPGLGPGALYQFEIRVRASGAVLLKSDPYAAMSELRPGKASRVAAP
                     ATHLWSDAAWLAARARWDWLHAPIAIYEAHLGSWRRHPDGCFYSYREAAEHLVPYVRE
                     MGYTHIELLPVTEHPLDESWGYQTTGYFAPTSRHGSPDDLRALVNVCHQAGIGVILDW
                     APAHFPRDESALAQFDGTALYEHEDPRLGFHPDWGTHVFNFGRNEVKSFLLSSAHCWL
                     AEFHADGLRVDAVASMLYLDYSRREGEWLPNRYGGRENLEAIDFLRELNVMVHREFPG
                     ALTLAEESTAWPMVSRPAHVGGLGFSMKWNMGWMNDTLAYFRHDPVHRRYHHDLLTFG
                     QLYAYTENFVLPLSHDEVVHGKASLLDKMPGDAWQKFANVRLLLTCQATAPGKKLGFM
                     GNEFAHGSEWRSDRELDWWLLDRPEHGGVQRLARDLNQLYHECAALHELDFEQAGFRW
                     VDCHDAEQSVVSFVRYARDGAFAVVVLNFTPVPRHGYRIGLPCAGTYRELFNSDSRHY
                     GGTDVGNGLVEARPEQWMGLPASAALTLPPLGGLILALA"
     gene            3009..>3586
                     /locus_tag="AC16CX_09910"
     CDS             3009..>3586
                     /locus_tag="AC16CX_09910"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3012863.1"
                     /translation="MTPELISKVGASPRLAPNLRDYDRSRAEFSWEQAAQSLSGLPGG
                     CGINIAHEAVDRHAASEHADHLALRWLGRDGASRDITFRELAELTDRFASVLRRLGIG
                     AGSRVFVLAGRIPELYVAVLGALKNRCVACPLFSAFGPEPIHTRLALGEGTVLVTTES
                     LYRRKVAGLRERLPGLRYILVISEGGEIPLTPG"
BASE COUNT          571 a         1239 c         1172 g          604 t
ORIGIN      
        1 cgtcgaagcg cccgtcgagg aagctccagc cgcgctggat atggcggatc aggctgtgcg
       61 ccttgtactg ggtgacgacg ccgatgcggc gcacgccgga attgacgcag ttcgaaagcg
      121 tgaaatcaat gatgcggaat ttgccgccga acgacaccgc gggcttggcc cgctcgctgg
      181 tgagctgcgc gagccggctg ccgcgcccgc ccgcgaggat gaacgcgaac gaagtcttgg
      241 tcagctggct gatgaacctg tggctctcca aatgcgcgcg catcgcctga tcctcctcaa
      301 tcgctcccgt cccgtcgtgc ggtcacgtag cagcctgtcg gacttatcag tccgcacagg
      361 ctgctaacag caaaaccggc tgaccgcttg agcaaaaacc cggccgaagg ggtcgaatgt
      421 gtagaaaagc gaaggtgatc ttcgcatatt cgtcatcctt ccttgcaatt ttcctggtgc
      481 cggttttgcg tcaggagttt gtcggggcca actccgtcaa actcctaccg gctacaatag
      541 cccgtaaccc cttctcggtc cccctcggca tgcaattcac ggacgagcgc acggaacgcc
      601 tgctggccgc gaccagtcac gaccccttcg cctgcctcgg gccgcagcgc gaaggcgcgg
      661 gctgggtcgt ccgcgtgttc cggccgcggg ccgaggcggt ggcgatgcag ggcgcagccg
      721 gcgccgcggc gttcgcgcgc gtgcattccg cgggcatctt cgagtggcgc agtccgcagc
      781 ctccccctca cccgtaccgc ctgctcgtca cggaaaacgg acgggcgcgc agcgagcacg
      841 acccctacgc gttcccgccc cggccttcgg cccacgatct ctatctgttc agccagggca
      901 ccaactacca agcctaccgc atcctcggcg cgcgctgcga gacccgcgag ggtgtcgcgg
      961 gcgtgcgctt ccgcgtgtgg gcgccgaacg ccgagcgggt gtcggtggtg ggcgacttca
     1021 acgaatggga cggccgcgtc catccgctgg ctaccctcgg agcgagcggc gtgtgggagc
     1081 tgttcgtccc cgggctcggt ccgggcgcgc tgtaccagtt cgagatccgg gtccgggcat
     1141 ccggcgcggt cctgctgaag agcgatccct atgccgcgat gagcgagctg cggccgggca
     1201 aggcctcgcg cgtcgccgcg cccgccaccc acctctggag tgacgcagcc tggctcgctg
     1261 cccgcgcgcg gtgggactgg ctgcacgcgc cgatcgccat ctacgaagca catctcgggt
     1321 cgtggcgaag gcatcccgat ggctgcttct acagctatcg cgaagccgcc gagcacctcg
     1381 ttccctacgt gcgcgagatg ggatacacgc acatcgagct gctgccggtg accgagcatc
     1441 ccctggacga atcgtggggc taccagacca cgggctactt cgcgccgacc agccgtcacg
     1501 gttcgccgga cgatctgcgg gcgctggtga acgtgtgcca tcaggccgga atcggcgtca
     1561 tcctcgactg ggcgccggcg cattttccgc gggacgagtc cgcgctggcg caattcgacg
     1621 gcaccgccct ctacgagcac gaggatccgc ggctcgggtt ccacccggac tggggcaccc
     1681 acgtcttcaa tttcgggcgc aacgaagtga agagctttct cctgtcgagc gcgcactgct
     1741 ggctcgcgga attccacgcc gacgggctgc gcgtggacgc cgtcgcctcc atgctctacc
     1801 tcgattactc gcgccgggag ggcgagtggc tgccgaaccg ctacggcggc cgggaaaacc
     1861 tcgaagccat cgacttcctg cgggagctca acgtgatggt gcatcgcgaa ttccccggcg
     1921 cgctgaccct ggcggaagag tccaccgcgt ggccgatggt atcgcggccg gcccatgtcg
     1981 gcggcctcgg cttctccatg aaatggaaca tgggatggat gaacgacacc ctcgcctatt
     2041 tccgccatga cccggtgcac cgccgctacc atcacgacct gctcacgttc ggccagctct
     2101 acgcctatac cgagaacttc gtgctgccgc tctcgcacga cgaggtggtc cacggcaagg
     2161 cctcgctcct cgacaagatg cccggcgacg cgtggcagaa attcgccaat gtgcgcctgc
     2221 tgctcacgtg ccaggcgacg gcgcccggca agaaactcgg gttcatgggc aacgaattcg
     2281 cccacggcag cgagtggcgc agcgaccgcg agctcgactg gtggctgctc gaccgtcccg
     2341 agcacggcgg cgtgcagcgc ctcgcgcgcg acctcaacca gctctatcac gaatgcgccg
     2401 cgctgcatga gctcgacttc gagcaggccg gctttcgctg ggtcgattgc catgacgcgg
     2461 agcagtcggt ggtgagtttc gtgcgctacg cgcgcgacgg cgcgttcgcc gtggtcgtcc
     2521 tcaatttcac gccggtcccg cggcacggct accgcatcgg tctgccctgc gccgggacct
     2581 accgcgagct tttcaacagc gattcgcgtc attacggtgg cacggatgtc ggaaacggcc
     2641 tcgtcgaggc gaggcccgag cagtggatgg ggctgcccgc ctcggcggcg ctcacgctgc
     2701 cgccgctcgg cggcctgatc ctcgccctgg cataaccgca gccctggccg gtgccgccgc
     2761 ggccggcctt ggttccagcc agctgcgact cgtcgggctc aacactcaac attcaacact
     2821 cataataata tggcacgcct tcgtggcagg cggttgacgt ggatcaatcc gacccgcatg
     2881 aagtagcgga aacgggttcc ggccgggggg cgggaaacgc cttgatctga gtcaataccg
     2941 tccggtgcgt gcgcgcctat gctgttgtgc ggtcgaagca tcgggcagga atcctcgaag
     3001 gcggcaaatt gacaccggaa ctcatcagca aggtgggggc gagcccgcgg ctcgcgccca
     3061 acctgcgcga ttacgatcgg tcccgcgccg aattctcgtg ggaacaggcg gcgcagtcgc
     3121 tgtcgggcct gcccggcggt tgcggcatca atatcgcgca tgaggcggtg gaccgccacg
     3181 ctgccagcga gcacgccgat cacctcgccc tgcgctggct tgggagagac ggcgcgagcc
     3241 gggacatcac attccgcgag ctcgccgagc tgacggaccg tttcgcgagc gtcctgcggc
     3301 ggctcggcat cggcgcgggg agccgcgtgt tcgtcctcgc cggccgcatt cccgagctgt
     3361 acgtggcagt gctcggcgcg ctgaagaacc gctgcgtcgc ctgcccgctg ttctccgcat
     3421 tcggacccga gccgatccac acgcgccttg ccctcggcga gggcacggtc ctcgtcacca
     3481 ccgagagcct ctaccgcagg aaggtcgccg gattgcgcga acgccttccc gggctgcgct
     3541 atatcctcgt catcagcgag ggcggtgaga taccgctgac gcccgg
//
LOCUS       DBNGWZ010000345         2984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2938104, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000345 DBNGWZ010000000
VERSION     DBNGWZ010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2938104"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1001)
                     /locus_tag="AC16CX_09915"
     CDS             complement(144..1001)
                     /locus_tag="AC16CX_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3012864.1"
                     /translation="MIICRFDNDRLGLVEGDAVRDVTPALDHLPRHAYPLPRFDPLIA
                     NLAAIRRQIESLSGNAPRRPIAAVKLLSPIANPGKILAAPVNYRKHLEEAREDPAIHH
                     QKQVGEIQKVGLFLKATSSLVGPGEGIALKHLDRRNDHEVELAVVIGKAGRDIPRAQA
                     FEHIAGYCIGLDMTVRGPEERSLRKSVDSFTVLGPWLVTADAVANPSALDLSLTVNGE
                     PRQKANTRDLILGIPELIEFATSFYTVHPGDVLLTGTPEGVGPVKPDDRLVAEIQGIG
                     RMEVLVRAA"
     gene            1291..>2984
                     /locus_tag="AC16CX_09920"
     CDS             1291..>2984
                     /locus_tag="AC16CX_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3012865.1"
                     /translation="MNLDTIKFSVLLYGMLQALRFTAWRRPSFARRLAEKNFTAQIKT
                     FDNEVGRYFTFREGKLASGRGIHPRPDISISVASAGLGARLFVPWVDQLQRIEAMKNF
                     DFKADGPDDLVVWFTQTLGMMVSVRWEYGTPMSDGTTRYVTNTNGGPLFVYVKDGKIV
                     RMTPLEFDETDARPWTIRARGKSFTPPHQTTIATHSLCSKTTLYSPDRLLYPMKRVDF
                     DPKGGRNPKNRGISGYERISWDEALDLVAAEIKRCKTQYGKGAIMVNHGSHHTWGNLG
                     YYISSAFKFFNIIGSTKVVHNPDSWEGWYWGAMHHFGYSMRLGQTETYSTVEDLMKEA
                     EMVVFWAADPEATSGSYSAYEGTIRRQWLHELGIEVVHIDPYYNHTAALLGGKWFAPR
                     PGTDAAMALAIAHVWITEGLYDRKFVAERSVGFEVWRDYILGKEDGVPKTPEWQEPET
                     GIPAKDVRALARRWGTKKTYLGAGGWGNGHGGACRSATGIQWARALACLLAMQGMGRP
                     GVNYGHLQWGTPIDLHFYFPGYADGGLSGDLEGTGMPISLYQRMPQLPTFNPSTQKV"
BASE COUNT          557 a          975 c          957 g          495 t
ORIGIN      
        1 ggcttggcgt caaccagctc gcgcggtgcg aaatgatgct gaaagtcgat gaccatcgcg
       61 ttgatcggct ggcgctgcgc ctctcctccg atcgttatcg caccatatta catgaacgaa
      121 acgggaaacc ggttggaacg gcactaagcc gcgcggacca acacctccat ccgcccaatg
      181 ccttgtatct ccgcgaccag ccggtcgtcc ggtttcacgg gaccgacgcc ctcgggcgtg
      241 cccgtcagca gcacgtcgcc cggatgcacc gtgtagaagg aggtcgcaaa ctcgatcagc
      301 tcgggaatgc cgagtatcag gtcgcgcgtg ttcgctttct gccgcggctc cccgttcacc
      361 gtgagcgaca agtcgagcgc ggaggggttg gcgacggcgt cggcggtcac gagccagggg
      421 cccaggacag tgaaactgtc cacggacttg cggaggctcc gctcttccgg cccgcgcacg
      481 gtcatgtcga gcccgatgca gtagcccgcg atgtgctcga acgcctgcgc gcgcggaata
      541 tcccgccctg ccttgccgat gaccaccgcc agctcgactt cgtggtcgtt gcggcggtcg
      601 agatgcttga gcgcgatccc ctcgccgggg cccacgagcg agctcgtcgc cttcaggaac
      661 aggcccacct tctggatctc gccgacctgc ttctgatggt ggatcgccgg gtcttcgcgc
      721 gcttcctcga ggtgcttgcg atagttcacc ggcgcggcaa gaatctttcc cgggttggcg
      781 atcgggctga ggagcttcac cgcggcaata ggccggcgcg gcgcgttgcc ggacaggctt
      841 tcgatctgcc gccggatcgc ggccagattg gcgatgagcg gatcgaagcg cggcagcggg
      901 taggcgtgac ggggcaggtg gtcgagcgcc ggcgtcacat cgcgcacggc gtctccctcg
      961 acaaggccaa gccggtcgtt gtcaaatcgg cagatgatca cgcgatatca acccggatgc
     1021 cattcagttg cccgacaata ccaccttgcc ggcgccgcat ccgggcgtgg acgttaccgg
     1081 atgatccgcc gtatctaaaa tggcgggcag cgcttcgacg tatccccctt cgaagccgaa
     1141 cggggggaca atatcgcaag ttgacagccg gcagctgtca gttggcagta actgcaaact
     1201 gcccaatgca aactggagac tttactgggg cagcgcccgg atgagaagct gggctggagc
     1261 gcacaagccc cccgattgcg gagagcggac atgaacctcg atacgatcaa gttctcggtg
     1321 ctgctgtacg ggatgctgca ggcgctgcgg ttcacggcgt ggaggcgccc gagctttgcc
     1381 cggcgcctgg cggagaagaa tttcaccgcc cagatcaaga ccttcgacaa cgaggtcggg
     1441 cgctacttca ccttccgtga aggaaagctc gcttctggac ggggcattca tcctcgcccc
     1501 gatatcagta tctccgtggc ctcggccggg ctgggcgcgc ggctgttcgt tccctgggtg
     1561 gaccagctcc agcgcatcga ggcgatgaag aatttcgact tcaaggcgga cgggcccgac
     1621 gatctcgtgg tctggttcac ccagaccctc gggatgatgg tgtcagtcag atgggagtac
     1681 ggcacgccga tgagcgacgg cacgacgcgc tacgtgacga acaccaacgg cggcccgctc
     1741 ttcgtctacg tcaaggacgg caagatcgtc cgcatgacgc cgctcgagtt cgacgagacc
     1801 gacgcccggc cctggaccat ccgcgcgcga ggcaagagct tcacgccccc ccatcaaacc
     1861 acgatcgcga cgcacagcct gtgctcgaaa accacgctct actccccgga ccggctcctc
     1921 tacccaatga agcgcgtgga tttcgacccg aagggcggcc gcaacccgaa gaaccgcggt
     1981 atctcgggct acgagcgtat cagctgggac gaggcgctcg atctcgtcgc ggccgagatc
     2041 aagcgctgca agacgcaata cggcaagggc gcgatcatgg tgaaccacgg gtcgcaccac
     2101 acctggggca acctcggcta ttacatcagc tccgccttca agttcttcaa catcatcggc
     2161 agtacgaaag tcgtgcacaa cccggacagc tgggaaggct ggtactgggg cgcgatgcac
     2221 cacttcggct acagcatgcg gctcggccag accgagacct acagcaccgt cgaggacctg
     2281 atgaaggaag cggagatggt ggtgttctgg gcggccgacc ccgaggcaac gagcggctcc
     2341 tacagtgcct acgagggcac catccgccgg cagtggctgc acgagctggg catcgaggtc
     2401 gtgcacatcg atccctacta caaccacacc gcggcgctcc tcggcggcaa atggttcgcg
     2461 cccaggcccg gcaccgacgc cgccatggcg cttgccattg cccacgtctg gatcaccgag
     2521 gggctctacg acaggaaatt cgtcgccgag cgctcggtcg gtttcgaggt gtggcgcgat
     2581 tacatcctcg gcaaggaaga cggcgttcct aagacccccg agtggcaaga gcccgagacc
     2641 ggcatcccgg cgaaggacgt gcgcgcgctc gccagaagat gggggacgaa gaaaacctac
     2701 ctcggcgccg gcggctgggg taacggccat ggtggggcgt gccgctcggc gaccggcatc
     2761 cagtgggcgc gcgcgctcgc gtgcctgctc gccatgcagg gcatggggcg ccccggcgtg
     2821 aactacggcc acctgcagtg gggtacccca atcgatttgc atttctattt cccgggctac
     2881 gccgacggcg ggctgtcggg cgacctcgag ggcacgggca tgccgatcag cctctaccag
     2941 cgcatgccgc agctgcccac cttcaacccc tcgacccaga aggt
//
LOCUS       DBNGWZ010000346        10314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3441253, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000346 DBNGWZ010000000
VERSION     DBNGWZ010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10314
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3441253"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1994
                     /gene="araD"
                     /locus_tag="AC16CX_09925"
     CDS             261..1994
                     /gene="araD"
                     /locus_tag="AC16CX_09925"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HIR3012866.1"
                     /translation="MARSRLKPEDLRSYRWFGGADLRATGHHSRALQMGYAREDFLGK
                     PVIGIINTWSDINPCHAHFRARAEEVKRGVWQAGGFPVEMPAIALAEPFQKPSTMLYR
                     NFLAMETEELLRSYPVDGAVLMGGCDKTTPALIMGATSMGLPFIYMPAGPMLRGNWRG
                     EPLGSGTDRWKYWAELRAGRITEQEWQEVVDGIARSHGHCMTMGTASTMTSAAEALGL
                     TLPGAASIPAADANHPRMATACGRRIVEMVWEDLRPAEILTPAAFDNAITVVHALSGS
                     TNAVIHLVAMAGRAGVRLGLGRFDEIARRVPVLANIRPAGQYLMEDFYYAGGLRALLA
                     NLKENLNLDCLTVNARTLGENIEGAHIYNSDVIRNRGNPISAIGGLAVLRGNLAPDGA
                     VIKPLAAEPRLLKHAGPAVVFSNYQDLLARVDDESLKVYENSVLVLQNAGPLGAPGMP
                     EWGQLPIPKKLLRRGVRDMVRISDARMSGTSYGTCVLHVAPEAYVGGPLALVCDGDLI
                     ELDVGARRLELRVGEAELAKRRAEWKPRPPHYARGYGAIFARHVTQANLGCDLDFLHH
                     GAPTPEPEVHH"
     gene            2109..2882
                     /locus_tag="AC16CX_09930"
     CDS             2109..2882
                     /locus_tag="AC16CX_09930"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3012867.1"
                     /translation="MELPVNRFKRALKEGRPQIGMWSSLSSHIVAEILAHAGFDWVLL
                     DTEHSPNELPMVQSQLHAMTGGTATPVVRPAWNDMVLIKRYLDIGAQSLLLPYVQTVE
                     EAQNAVRYTRYPREGVRGVAGSTRAAGYGRIKDYMKRAHEELCVLVQAETRLALKNLE
                     AIAAVEGVEGVFIGPNDLAADLGHLGNWQHPDVWKAMEDAAKRIRKAGKAPGILVGEA
                     DGQRCLDMGFLFVAVGADVGMLARGADALAAKFKQAGRS"
     gene            2977..4347
                     /locus_tag="AC16CX_09935"
     CDS             2977..4347
                     /locus_tag="AC16CX_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3012868.1"
                     /translation="MKASSILSFVVVAAAIAVYLTPPPAGATPEMMHAAALVVFTVGL
                     WAVGALPEHITGLLFFMFAMALAVAPAQVVFSGFASATLWLVLGGLIMAEAVNRTGLA
                     QRLAGALFDRFASSYAKLVAAVVVACIALSFIIPATTGRVLLLLPIVMALARRVGFEH
                     GSTGYNGLCVATITATYQCGTTVLPANAPNLVFAGSAEALYGVQFTYTEYLWVQLPVL
                     GLLKGASIVAFTRWLFPAEARPVTDAAVSVPLTAGQRRMAAILAAALALWATDFLHGI
                     NPGWIALAAGIACLLPAVGVMPVTAFHEVRLGPYFYVGATLGLGLVMQKTGLSDGLGR
                     LLHGLLPLEAGADFANFIMLSVLTTLAGLFTTNPAQPAVLAPLAEYFSQATGWPLKAA
                     LMVIAVGFTTFLLPYQVPPVMVGLQVGALRVTTALRLALPLSAFSLVVLLPLQYLWWR
                     LIGYFG"
     gene            complement(4547..5521)
                     /locus_tag="AC16CX_09940"
     CDS             complement(4547..5521)
                     /locus_tag="AC16CX_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3012869.1"
                     /translation="MFTRIFGVALFMALGSASGVLAQDYPAKLVTIVVPFAAGGPTDT
                     LTRNLAQAMTGSLKQQVIIDNTVGAGGTIGAAKVAKARPDGYMLLVYHIGMATAPALY
                     RKLSFDPLNDFEPIGLISDVPMTLIANEKFPPNNLKELIAYVKANKDKISYGNAGLGA
                     ASHLCGLLFMSAIRTELNTVPYKGTAPALTALLGNEVQFVCDQTTNTTGHIQAKKVKV
                     YGVTTRTRLKAMGDIPTLHEAGLKNFEVAVWHGLWAPKGTPKPVVDRLVSALQAALKD
                     PAFVANLAKLATEPVAQNRATPAALQKHLKAEIDKWGPIIKKAGVYAD"
     gene            complement(5635..6399)
                     /locus_tag="AC16CX_09945"
     CDS             complement(5635..6399)
                     /locus_tag="AC16CX_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012870.1"
                     /translation="MSTFADSAAVRPLPLDPVSAWVGRARHHDATRALAITTVIAGGL
                     ALDQSIEFWGQMLTNIGIWAVLLHWLRGAAPRDQLAMGLCVLYATLGEIFLSLVWGLY
                     EYRLANVPLFVPPGHALLFMLGRILAERAREWIVWFVPLAAVPFVYLLAWTGLGTLDA
                     LLFALFLLCLLSGRAGRLYAVMFVLSLAMEIYGTWLGNWTWAEQAPWLGFTTINPPLA
                     AGAFYCMLDMLIVATVARMRLVPLKTGASLAFASSR"
     gene            complement(6399..7361)
                     /locus_tag="AC16CX_09950"
     CDS             complement(6399..7361)
                     /locus_tag="AC16CX_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012871.1"
                     /translation="MDRFVIGVAAALGLAAASVTAQDYPTRPVRLVVPYAPGGPVDIV
                     GRLTAQKLSEALAQQVVVENRAGAGGNIAVELVARATPDGHTLLMGANGTNAINPSLY
                     KKLPVDPEKDLAPISMVASSAMILVVHPSLPVASVKQLIALARAKPGAITYASSGSGS
                     TAHLSSELFKSMARIDLLHVPYKGAGPALTDLVAGHVQTMITAISSTLPYVKAGRLKA
                     LGVSSEKRLPILPEIPAVAEQLPGYEVITWYGVFAPAGTPRPVIDKLNQALVRVLATP
                     DAQGKLAALGADARTNAPEQFAESVRKERAKWARIIQESGARPE"
     gene            complement(7377..8732)
                     /locus_tag="AC16CX_09955"
     CDS             complement(7377..8732)
                     /locus_tag="AC16CX_09955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3012872.1"
                     /translation="MNASYDPLTARTPGSGLAHPPSYWTATATTMPADDGPVPAQVET
                     EVAIIGGGYTGLSCALFLARRYGIRTLVLEANRAGWGCSGRNGGFARAAIGRYSYAEI
                     IERWGRETARHWFGESLTAVNTVRELVRDNAIDCDLTEAGHLKIAHRPSRAAALAQEA
                     ELLQREFEYPAEFLSADRVQRDHIGGAESHGALRFPDALAVHPLKLAFGMLAEARAAG
                     AQVHSASPVLGWDKNNGTHILVTPYGTVRAKTVVIATNGYTPERLFPCLRGTTLPVLS
                     HIIVTRPMTPGEKKASGFVTGHVLTDTRNLLYYWRRMPDDRILFGGRGLIVESPAGHG
                     RQREFLLAELKRKLPALADTEVEHDWWGWVCLTADFLPHVHHAEDDRTVHYAIGYQGS
                     GVAYGLHAGRLLAQRVAGGGVETTIPPVSTPLPRFPLAALRRAGQRAAYLWYRYLDGR
                     N"
     gene            8869..9594
                     /locus_tag="AC16CX_09960"
     CDS             8869..9594
                     /locus_tag="AC16CX_09960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3012873.1"
                     /translation="MEGNAVVTGASSGIGEAIARHLLDSGCTVITLQRRPPRIQHERL
                     RFHAVDLADGAAARRAAGEIATRYSVCYLVNNAGVNRPGTLEQATTEDLDYAMAVNVR
                     AAMILIQAFAPGMRAAGFGRIVNIASRAALGKTGRAVYSAAKAGLIGMTRMLCLELAG
                     AGITVNAVAPGPVATELFDRGHPPGGEKRQRVIDSIPVRRVGTPDDIARAVVFLLSPE
                     SGYITGQTLFVCGGTSISGSGGA"
     gene            9718..9803
                     /locus_tag="AC16CX_09965"
     tRNA            9718..9803
                     /locus_tag="AC16CX_09965"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9752..9754,aa:Tyr,seq:gta)
     gene            9850..9923
                     /locus_tag="AC16CX_09970"
     tRNA            9850..9923
                     /locus_tag="AC16CX_09970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9882..9884,aa:Gly,seq:tcc)
     gene            9989..10063
                     /locus_tag="AC16CX_09975"
     tRNA            9989..10063
                     /locus_tag="AC16CX_09975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10021..10023,aa:Thr,seq:ggt)
     gene            10211..>10314
                     /locus_tag="AC16CX_09980"
     CDS             10211..>10314
                     /locus_tag="AC16CX_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3012874.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTM"
BASE COUNT         1794 a         3222 c         3462 g         1836 t
ORIGIN      
        1 atctcgccga tgcggtgcgt gaaggcatcg caagcggcaa accatgcgtg atcgacgcca
       61 atatcgacgg cgacctgaat cctgccgggg cggggatctg ggagctgccg ggcatgggcc
      121 gcagcaagcc ggcaatcggc aagcagtatg tgcccgaggg ctgacagtag ctgtcagcca
      181 agtcgaaagt cggaaggcga aagtcgaaag gtcttcccgc tgggcgggca ccactaaccc
      241 cataacctgt tacctaatca atggcacgat cgagactgaa gccggaagac cttcgaagtt
      301 accgctggtt cggcggagcc gacctgcgcg cgaccggcca ccactcgcgc gcgctgcaga
      361 tgggctacgc gcgcgaggac ttcctcggca agcccgtcat cgggatcatc aatacctgga
      421 gcgacatcaa tccctgccac gcccacttcc gggcgcgcgc ggaggaggtg aagcgcggcg
      481 tgtggcaggc cggcggattt cccgtcgaga tgcccgccat cgcgctcgcc gagccgttcc
      541 agaaaccctc caccatgctc taccgcaact tcctcgcgat ggaaacggag gagctgctgc
      601 gctcctaccc ggtggacggc gcagtgctga tgggcggctg cgacaagacg acgccggccc
      661 tcatcatggg cgcgacctcg atgggattgc cgttcatcta catgccggcg gggccgatgc
      721 tgcgcggcaa ctggcgcggc gagccgctcg gctccggcac cgaccgctgg aagtactggg
      781 ccgagctgcg cgccgggcgc atcacggagc aggaatggca ggaagtggtg gacggcatcg
      841 cgcgctccca tggccattgc atgaccatgg gcaccgcctc gaccatgacc tccgccgccg
      901 aagcgctcgg cctcacgctg cctggcgcgg cctcgattcc ggccgcggat gcgaaccacc
      961 cgcgcatggc gaccgcctgc ggccggcgca tcgtcgagat ggtgtgggag gatctccgcc
     1021 ctgcggagat cctaacgcct gcggcatttg ataacgcaat caccgtggtc catgcactgt
     1081 cgggatcaac caacgccgtg atccatctcg tggcgatggc cgggcgcgcg ggcgtacgtc
     1141 tcggactcgg ccgcttcgac gagatcgcgc gccgcgtgcc ggtgctcgcc aacatccggc
     1201 cggccgggca gtacctgatg gaggatttct actatgccgg aggtttgcga gccttgctcg
     1261 caaaccttaa ggagaatctg aatctcgatt gcctcaccgt aaatgctagg acgctgggcg
     1321 agaacattga aggggcgcac atctacaata gtgatgttat tcgtaatcgc ggaaatccca
     1381 tttccgcgat cggcggcctc gcggtgctgc gcggcaatct cgcgccggac ggcgccgtga
     1441 tcaagccgct cgccgccgag ccgcgcctgc tcaaacacgc agggccggcg gtcgtattct
     1501 ccaattatca ggacttgctg gcgcgggttg acgacgagag cctcaaggtg tacgagaact
     1561 cggtactggt cctgcagaac gccggcccgc tcggcgcccc gggcatgccg gagtgggggc
     1621 aactgccgat tccaaagaaa ctgctcaggc gcggcgtgcg cgacatggtg cggatctcgg
     1681 acgcacgcat gagcggcact tcgtacggca cctgcgtgct gcacgtcgcg cccgaggcct
     1741 acgtcggcgg accgctcgcg ttggtgtgcg acggcgatct catcgagctc gacgtcgggg
     1801 cgcgcaggct cgagctccgg gtgggcgagg ccgagctcgc gaaacgccgc gccgagtgga
     1861 agccgcgccc gccgcattac gcgcgcggct acggcgcgat cttcgcgcgc cacgtcacgc
     1921 aggccaacct gggctgcgat ctcgatttcc tgcatcacgg cgcgccgacg cccgagcccg
     1981 aagttcacca ctgacaacgg gtgataggtg ataagtgata ggtgataggg gttgacttcc
     2041 gctgcgcggg aatccttgtc acttatcact catcacttat cacgcttttt caagaggagc
     2101 gcagctcgat ggaattgccg gtcaacaggt tcaagcgcgc gctgaaggag ggcaggccgc
     2161 agatcggcat gtggtcgagc ctgtcgagcc acatcgtggc cgagatcctc gcgcacgccg
     2221 gcttcgactg ggtgctgctc gacaccgagc actcgcccaa cgagctgccg atggtgcagt
     2281 cgcagctgca cgcgatgacc ggcggcaccg ccacgccggt cgtgcgtccg gcgtggaacg
     2341 acatggtgct catcaagcgc tacctcgata tcggcgcgca atccctgttg ctgccgtacg
     2401 ttcagaccgt cgaggaggcg cagaacgcgg tgcgctacac gcgctacccc cgggagggtg
     2461 tgcgcggggt ggcgggctcg acccgcgccg cgggctacgg ccggatcaag gattacatga
     2521 agcgcgcgca cgaagagctg tgcgtactgg tgcaggccga gacgcggctc gcgctgaaaa
     2581 acctcgaggc gatcgctgcg gtcgagggag tggaaggcgt gttcatcggc cctaacgatc
     2641 tcgccgctga tctcgggcat ctcggcaact ggcagcaccc cgacgtgtgg aaggcgatgg
     2701 aggacgcagc gaagcgcatt cgcaaggccg gcaaggcgcc gggcatcctg gtgggcgagg
     2761 cggacggcca gcgctgcctc gacatgggtt ttctgttcgt cgcggtcggg gcggacgtcg
     2821 gtatgctcgc gcgcggcgcg gatgcactcg ccgccaagtt caaacaagcc gggagatcgt
     2881 gagagcggga aatcgggagt gcgggaaatc gaaagcagga gcttccgacc cgtcgctctc
     2941 ccgctctgac gccctctcgc ccttccggct cttccgatga aggcgtcctc gattctcagc
     3001 tttgtcgtcg tcgcggctgc gattgcggtt tacctgacgc cgccgcctgc gggagccacg
     3061 cccgagatga tgcacgctgc ggcgctggtc gtgttcaccg tcgggttgtg ggcggtggga
     3121 gcgctgcccg agcacatcac cgggctgctc ttcttcatgt tcgcgatggc gctggcggtg
     3181 gcgccggcgc aggtggtgtt ctccggattt gcctccgcca cgctgtggct ggtgctgggc
     3241 gggttgatca tggccgaggc cgtgaaccgc accgggctgg cgcagcggct ggcaggggcg
     3301 ctgttcgacc gcttcgcatc gtcctatgcg aaactcgtcg ccgccgtagt ggtggcgtgc
     3361 attgcgctgt cgttcatcat tccggcgacc accgggcgcg ttctgctgct cctgcccatc
     3421 gtgatggcgc tcgcacggag ggtgggcttc gagcacggca gcaccggcta caacggactc
     3481 tgtgttgcga cgatcacggc cacttaccag tgcggtacga cggttctgcc cgccaatgcg
     3541 cccaacctgg tgtttgccgg ctcggctgaa gcgctctatg gcgtgcagtt cacctacacc
     3601 gagtacctgt gggtccagtt gccggtactg ggcctgctga agggcgcctc gatcgtcgcg
     3661 ttcacgcgct ggctgtttcc cgccgaggcg cggccggtga cggacgcggc cgtttcggtt
     3721 ccgctcacgg ccgggcagcg gcgcatggcc gcgatcctcg cggcggcgct cgcgctgtgg
     3781 gcgaccgatt tcctgcacgg gatcaatccg ggctggatcg cgctcgcggc gggcatcgcg
     3841 tgcctgctgc cggccgttgg cgtgatgccg gtcacggcgt tccacgaggt gaggctgggt
     3901 ccgtatttct acgtcggcgc aacgctcggg ctcggactgg tcatgcagaa gaccggcctg
     3961 agcgacgggc tcggcaggtt gctgcacggc ctgctgccgc tcgaagccgg cgccgatttc
     4021 gccaatttca tcatgctgtc ggtgctgacg acgctcgcgg ggctgttcac caccaatccg
     4081 gcgcaaccgg cggtattggc gccgcttgct gaatacttct cgcaggccac cggctggccg
     4141 ctgaaggcag ccctgatggt gatcgcagtg ggtttcacga ccttcctgtt gccgtatcag
     4201 gtgccgccgg tcatggtggg gctgcaggtg ggcgcgctgc gggtgacgac cgcgctcagg
     4261 ctcgcgctcc cgctctcggc gttcagcctg gtcgtgttgc tgccgcttca gtacctgtgg
     4321 tggcggctaa tcggctattt cggctgagca gccagcctgt cgaactgcag ttgatgagaa
     4381 tggtgcgctg taatgacgga cgcgctatgg acagccgcta tagcgacagg ctgtagataa
     4441 agtacatttt ctggcacttt cgttccagca ccctgttacg ccaaaatccc gacagcccac
     4501 tatccgatct cgttaaataa aatccccggg gcgccgggta ttttattcag tcggcgtaga
     4561 cgcccgcctt cttgatgatc ggtccccact tgtcgatctc ggccttgagg tgtttctgca
     4621 aggccgctgg cgtggcccga ttctgcgcaa ccggctcggt ggcaagcttc gcgaggttcg
     4681 cgacgaacgc cgggtccttg agcgccgcct gcagcgcgga aaccagcctg tcgaccaccg
     4741 gcttgggcgt gcctttcggc gcccacaggc cgtgccatac cgcaacctcg aagttcttga
     4801 gcccggcttc atgcagcgtc ggaatgtcgc ccatggcctt cagacgcgtg cgggtggtga
     4861 cgccgtacac cttcaccttt ttcgcctgga tgtggccggt ggtattggtg gtctggtcgc
     4921 atacgaactg gacttcgttg ccgaggagtg cggtcagcgc cggcgccgtg cccttgtacg
     4981 ggacggtgtt gagttcggtc cggatcgcgc tcatgaacag caggccgcac agatgcgaag
     5041 ccgcgcccag ccccgcgttg ccgtacgaga ttttgtcctt gttggccttc acgtaggcga
     5101 tcagctcctt caggttattg ggcggaaact tctcgttcgc gatcaatgtc atcgggacgt
     5161 cggagatgag cccgatcggc tcgaaatcgt tgagagggtc gaacgacagc ttgcggtaaa
     5221 gcgccggcgc agtagccatg ccgatgtgat atacgaggag catataacca tcgggcctcg
     5281 cctttgcgac cttcgccgcc ccgatcgttc cgcccgctcc cacggtgttg tcgatgataa
     5341 cttgctgctt cagcgatccc gtcatggcct gcgcaaggtt gcgtgtgagg gtgtcggtgg
     5401 gaccgccggc agcgaaggga acaacgatcg tgacgagttt ggccggatag tcttgggcga
     5461 ggacgcccga tgccgagcct agagccatga atagcgcgac gccgaaaatg cgagtgaaca
     5521 tggcgtgtct cctcagtgac gaatcgactg aaacaagctc ccaagagggg agggatattc
     5581 gcggcgtgtt gtctgccgca cgggaggata taaatcttac cagagaccgc gagcttaccg
     5641 tgatgaagcg aacgcaagcg aagccccggt cttgagcgga accagccgca tcctggcgac
     5701 ggtggccacg atcagcatat cgagcatgca gtagaacgct ccggctgcaa gtggcggatt
     5761 gatggtggtg aaaccgagcc agggggcttg ctccgcccat gtccagttgc cgagccaggt
     5821 gccgtagatc tccatcgcca gcgagagcac gaacatcacc gcgtatagcc ggcccgcgcg
     5881 cccggacagc agacagagca gaaacagggc gaacagcaac gcgtccaagg tacccagccc
     5941 ggtccatgcg agcaggtaca cgaaggggac ggcggcgagc ggcacgaacc acacgatcca
     6001 ttcgcgcgca cgttcggcca ggatgcggcc cagcatgaac agcagcgcgt gccccggcgg
     6061 cacgaacagg gggacattgg cgagccggta ttcgtagagt ccccacacca gggacaggaa
     6121 gatctctccg agcgtcgcgt acagcacgca aaggcccatc gccagctggt cacggggcgc
     6181 cgcgccacgc agccagtgca gcaggaccgc ccagatgccg atgttggtca gcatctgtcc
     6241 ccagaactcg atactctggt cgagcgcgag gcccccggca atgactgtgg tgattgcaag
     6301 tgcgcgggtg gcgtcgtggt gccgcgctcg gccgacccac gcggaaaccg ggtcgagggg
     6361 cagcgggcgc acggcggcgc tgtcggcgaa cgtgctcatc actcggggcg cgcgccggac
     6421 tcctggatga tccttgccca tttcgcccgt tccttcctga ccgattccgc aaattgctcc
     6481 ggcgcgttgg ttcgggcatc agcgcccagc gcggcgagct tgccctgtgc atccggcgtg
     6541 gccaggaccc tgacgagggc ctggtttagc ttgtcgatga ccggccgcgg cgtgcctgca
     6601 ggcgcgaaca cgccgtacca agtgattact tcgtatcccg gcagctgttc ggcgacggct
     6661 ggaatctcgg gcaggatcgg cagccgtttc tcgctcgaga cgccgagcgc cttgaggcgg
     6721 cccgctttca catagggcag ggtcgaagag atggcggtga tcattgtttg cacgtgcccg
     6781 gcgacgagat ccgtcaacgc cggccccgcg cccttgtatg gcacgtgcag cagatcgatc
     6841 ctcgccatgc tcttgaacag ctcgctcgaa aggtgcgccg tggacccact gccggaggag
     6901 gcgtaggtaa ttgcccccgg tttggcccgg gcaagcgcga tcagctgctt gacactggcc
     6961 accggcaacg aaggatgcac gaccaggatc atcgcgctgg aggccaccat gctgatcggc
     7021 gcaagatcct tttcgggatc caccggcagt ttcttgtaaa ggctggggtt gatcgcattt
     7081 gttccgttcg cgcccatgag cagcgtgtga ccgtcgggcg tggcgcgcgc gacgagttcc
     7141 accgcgatgt tgcctccggc gccagcgcgg ttctcgacga cgacttgctg ggcaagcgct
     7201 tccgagagct tctgtgcagt gaggcgcccc acgatgtcca ccggcccgcc cggcgcgtac
     7261 gggacgacca gccgcaccgg gcgcgtggga tagtcctgtg cagtgacgct cgcggcggca
     7321 agccccagcg ccgctgcaac accgataacg aagcggtcca tgtctgtccc ccgggtctag
     7381 ttcctgccgt ccagataacg gtaccacagg tacgccgcgc gctgaccggc gcggcgcaag
     7441 gcggccagcg gaaagcgcgg cagcggggtg gaaacggggg ggattgtcgt ttctacgcct
     7501 ccgccggcga cgcgttgtgc aaggagccgg cccgcgtgca atccgtaggc gacgccgctc
     7561 ccctggtagc cgatggcgta atgcaccgtc ctgtcgtcct cggcgtgatg gacgtgcggc
     7621 agaaagtcgg cagtaaggca cacccagccc caccagtcat gctcgacctc ggtgtcagcc
     7681 agcgcgggaa gcttgcgctt gagctcggcg agcagaaatt cccgctggcg cccatgaccc
     7741 gccggggact cgacgatcag ccctcgtcca ccgaagagga tgcggtcgtc cggcatccgc
     7801 cgccagtaat agagcaggtt acgggtgtcg gtgagcacgt ggcccgtgac gaagccgctt
     7861 gccttctttt cccctggcgt catggggcgg gtgacgatga tgtgcgacag caccgggagc
     7921 gtggtgccgc gcaggcacgg gaacaggcgc tcgggcgtgt agccgttcgt ggcgatgacg
     7981 accgtcttcg cgcgcaccgt tccgtatggc gttaccagta tgtgcgtgcc gttgtttttg
     8041 tcccagccga gaacggggct cgcgctgtgc acctgtgcgc ccgccgccct tgcctctgcg
     8101 agcatgccaa atgcaagctt gagcggatgc acggcgagcg catctggaaa gcgcagcgca
     8161 ccatgcgatt cggcgccccc gatgtgatcg cgttgcaccc gatcggcgga caggaactcg
     8221 gccgggtatt cgaactcgcg ctgcagcagc tccgcctcct gagcgagtgc ggctgcgcga
     8281 ctgggtcggt gcgcgatctt cagatgtccg gcctcggtca ggtcgcagtc gatggcgttg
     8341 tcccggacca gctcgcgcac agtgttgacg gctgtgaggc tttcgccgaa ccagtggcgt
     8401 gccgtctcgc gcccccatct ctcgattatt tccgcatagc tgtaacgccc gatcgcggcg
     8461 cgcgcaaagc ccccgtttct gccgctgcaa ccccagccgg cgcggttcgc ctccagcacc
     8521 agcgtcctta tgccgtagcg cctggcaagg aagagcgcgc aggagagacc ggtatagcca
     8581 ccgccgatga tcgctacctc ggtttcgact tgcgccggca cggggccgtc gtcggcgggc
     8641 atagtggtcg ccgtcgctgt ccagtacgaa ggcggatgcg cgagtccgct cccgggcgtg
     8701 cgcgcggtaa gcggatcgta ggaagcgttc acggcaaaac ggcggggcgg ctttgaatgc
     8761 cgagatcgag gaggcggggt cccatggtat cagaccggtg gcgggcggac ataaagaagc
     8821 gtgtcccggc aacgatataa tcgcgccaat ttgccgctgg gggaaatcat ggaaggcaat
     8881 gccgtagtga cgggagccag ctcgggcatc ggagaggcca tcgcgcggca tctgctcgac
     8941 agcggctgca cggtgatcac tttgcagcgc cgaccgccgc gcattcaaca cgagcggctg
     9001 cggtttcacg ccgtggacct cgctgacggc gctgctgcgc gcagagcggc tggtgaaatc
     9061 gcgacccgct attcagtctg ttacctggtg aacaatgccg gcgtcaaccg cccgggcacg
     9121 ctggagcagg cgacgaccga ggatctcgat tacgcaatgg ccgtaaatgt ccgcgccgca
     9181 atgatcctga tccaggcgtt cgcgcccggc atgcgcgccg ccggattcgg ccgcatcgtc
     9241 aacatcgcct cgcgcgcagc gctcggcaag accgggcgcg ccgtgtactc cgcggccaag
     9301 gcggggttga tcgggatgac acggatgctg tgcctggagc tcgccggcgc cggcattaca
     9361 gtcaacgcgg ttgcgcccgg accggttgcg accgagctgt tcgaccgtgg gcacccgccg
     9421 ggcggcgaga agcgccaacg cgtgatagac agcattcccg tcaggcgggt tggcaccccc
     9481 gacgacatcg cgcgcgcagt agtgttcctg ctttcgcccg agagcggcta catcacgggc
     9541 cagacgctgt tcgtgtgcgg gggaaccagc atcagtggct ccggcggggc ttagtagaag
     9601 agagtgtcgg cgggtccagg cctgcccccg cgccgatggg atgcagaaat tactccaaaa
     9661 tcggtgttag gcgtttgaca atatgggaga aaacaacaat aatgcccggt ttcgttcgga
     9721 ggggttcccg agcggtcaaa gggggcagac tgtaaatctg ttggcttacg ccttcgaagg
     9781 ttcgaatcct tccccctcca ccaccaggat tttcgcgggt aaagagggct caaggatact
     9841 cgaagcagcg cgggtgtagc tcaatggtag agcagaagcc ttccaagctt acgacgaggg
     9901 ttcgattccc ttcacccgct ccagaaattt cgaaggactg cggacgacgg gcgacagggg
     9961 ctcggacaag cggagattct ggcggcaggc ccatgtagct cagtggtaga gcactccctt
    10021 ggtaagggag aggtcgcgcg ttcaatccgc gccgtgggca ccaaaattcg ccttcgacaa
    10081 aattcgaagg tgacgaattc tgaatagagg aactactttt atttttttgc ggattcagcg
    10141 cgtcgaggtt ggttgatcgg gatagattgc gggcaagagg gcctaacggc acaaagagtg
    10201 gagcaaagag atggcaaagg gcaaatttga gcgcaccaag ccgcacgtga acgtgggcac
    10261 gattgggcac gtggatcatg gcaagacgac gctgacggcg gcgatgacga tgat
//
LOCUS       DBNGWZ010000347         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1048175, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000347 DBNGWZ010000000
VERSION     DBNGWZ010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1048175"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="AC16CX_09985"
     CDS             <1..413
                     /locus_tag="AC16CX_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3012875.1"
                     /translation="PTDAGTKSVVAAVSGSKPETPSEATDLLEARLVATREWLATEAH
                     TTFSIQIMGANDPEQLKEHLNSITKFVEINKVFVYRTVAKQKPSLTVLYGSFSSPRAA
                     QEALDKLPESLRANRPILRTVQGIRAEIRQHQSS"
     gene            593..2353
                     /locus_tag="AC16CX_09990"
     CDS             593..2353
                     /locus_tag="AC16CX_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HIR3012876.1"
                     /translation="MVLALILTATGCANIKPYIPPSEGHITAHPAAKAEPDRAIPPPA
                     RVSEFVPPPTPAVKPQTYSVVVNEVPVKDLLLALARDTKQNIDIHSGLTGLVSLNAIN
                     ETLPAILERVSKQVNMRYRLEGNTIMVSPDLPYVKTYRVNYVNMARDTTSTIGVTGEI
                     ATSSGGPGAAGAGATTSGSSTVVRTTSKNDFWEQLRDNIRAILNSTRLQSLTAEAKAE
                     RLALVKQEQEIRAKQIEAASRAGQGAPTLAASVISGPPGAAAQQQATLLPDDVIVNAI
                     SGTVTVNATEKQHQLVQQHIDSIVNAMQRQVLIEATIVEVQLSNNYQGGIDWAKVSQS
                     GGIAFTQSLLGGNLATAPFFALFYNQNNQRPQDIQLTVKLLEQFGNTRVLSSPKLMAL
                     NNQTALLKVVDNIVYFEVQAQTTQSQTTALTTATTTPKTVAVGVVMGVTPQINEDGRV
                     SLTVRPTISRVLRFINDPNPALAQAGVTNSVPEVQTREMESVLQVGNGQTVILGGLMQ
                     DDVRRSRDQIPGADRAGAAGELFRFRDEQVSKTELVIFLRPTVVANPSLDSDELRFFQ
                     RFLPKPEATPPQPEKTGAAQ"
     gene            2350..>2654
                     /locus_tag="AC16CX_09995"
     CDS             2350..>2654
                     /locus_tag="AC16CX_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012877.1"
                     /translation="MSLLMKALEKAAKDRGEARNEPAVAAAAPAQAKAELSLEPLAVE
                     APPPKAEAAVRPAVAPRAAAVPPRAAAAPAAPVREQAQAQAATVLQAQAASRPAAGGA
                     "
BASE COUNT          594 a          865 c          786 g          409 t
ORIGIN      
        1 ccccaacgga tgccggaaca aaatccgtag tcgctgctgt tagcggcagc aaacctgaga
       61 caccttcgga ggcgaccgat ctgctcgaag caaggcttgt cgccacgcgc gagtggctgg
      121 cgaccgaggc tcacaccact ttcagcatcc agatcatggg cgccaatgac ccggaacagc
      181 ttaaggagca cctcaattcg ataacaaagt tcgttgaaat aaataaggtt tttgtgtatc
      241 gtaccgtggc aaagcagaag ccgtccctga ccgtcttgta cggcagcttc agcagcccgc
      301 gtgcagcgca ggaagcgctt gataaactgc cggaatcgct ccgggcgaac cggccgatac
      361 tacgcacggt gcaaggcata cgcgccgaga tcaggcagca ccagtcgtcc tgaccgcaat
      421 gcatacgagc tcgtgacgag tcaagcacag caagagccaa ggaagaaggg gggaaatgcg
      481 aacgactgaa gcagcgcaag gaatttgccg gattttcaat cgacaaattc ctgaagtgtt
      541 ggaaccgggg aagagaaacg cagcgccgta ttttccgcgc tcctgtttcc cgatggtgct
      601 ggcgctgatt ctgaccgcaa caggctgcgc gaacatcaaa ccctacatcc ctccctcgga
      661 aggccacatc accgcgcatc ccgcggcaaa agccgagccg gacagggcca ttcccccgcc
      721 cgccagggtg agcgagttcg tgcccccgcc cacaccggcg gtgaagccgc agacctacag
      781 cgtggtggtc aatgaggtgc cggtgaagga cctgctgctc gcgctcgcgc gcgacaccaa
      841 gcagaacatt gacattcact ccgggctcac cggtctcgtc agtctcaatg cgatcaatga
      901 gacgctgccg gcgatcctcg agcgcgtctc gaagcaggtc aacatgcgct accgccttga
      961 aggcaacacc atcatggtgt cgcccgatct tccctacgtg aaaacctatc gcgtgaacta
     1021 cgtgaacatg gcgcgcgaca cgacctcgac catcggggtc acgggcgaga tcgcgacctc
     1081 cagcggaggt ccgggtgcgg cgggagccgg cgcaacaacc agcggctcaa gcacggtggt
     1141 gcgcacgact tcgaaaaacg atttctggga gcaattgcgg gataacatcc gcgccatcct
     1201 caattccacg cgcttgcaaa gcctgaccgc cgaagccaag gccgaacggc ttgccctcgt
     1261 caagcaggaa caggaaatcc gggcgaagca gattgaagca gcaagccgcg ctggccaggg
     1321 cgcgcccacc ttggccgcat cggtgatatc gggaccgcca ggtgccgcag ctcagcaaca
     1381 agctacgctg ttgcctgacg atgtgatcgt caacgcgata tccggcacgg taaccgtcaa
     1441 cgcgaccgaa aagcagcacc aactggtgca gcagcatatc gacagcatcg tcaatgccat
     1501 gcagcggcag gttttgatcg aagcgacgat cgtggaagtg cagctctcca acaactacca
     1561 ggggggcata gactgggcca aagtgtcgca gagcggcggc attgccttta cccaatcctt
     1621 gctcggcggc aatctcgcca ccgcaccgtt cttcgcgctt ttctacaacc agaacaacca
     1681 gcgcccgcag gacatccagc tcacggtcaa gctcctcgag cagttcggca atacgcgcgt
     1741 gctatccagc cccaagctga tggcactcaa caaccagacg gccttgctta aggtcgtgga
     1801 taacattgtt tatttcgaag tccaggcgca aaccactcaa agtcagacaa cggcgctgac
     1861 cacggcgacg accactccca agactgtcgc agttggcgtg gtcatggggg tgacgccgca
     1921 gatcaacgag gatggtcgcg tgtcgcttac cgtgcggcca acaatctcac gcgtgctgcg
     1981 gttcataaac gacccgaatc ccgcattggc gcaagcaggt gtgaccaact ccgtgccgga
     2041 agtgcaaacg cgtgaaatgg aatccgtgct gcaagttggc aatggccaga ccgtcatcct
     2101 gggggggctg atgcaggacg acgtgagacg cagccgcgat cagatccccg gcgcagaccg
     2161 ggccggcgcg gccggcgagt tgttccgctt ccgcgatgag caggtcagca agaccgaact
     2221 ggtgatcttc ctgcgcccga ccgtcgtcgc caatccttcc ctggacagcg acgagctcag
     2281 attcttccaa cgcttcctgc cgaaacccga ggctaccccg ccgcaacccg agaagaccgg
     2341 cgccgcgcaa tgagcctgtt gatgaaggcc ttggaaaagg ccgccaaaga ccggggggaa
     2401 gcacgcaacg agccggccgt ggccgcggcg gctccagcgc aggcaaaggc ggaactctcc
     2461 ctcgagccgc tggccgtcga agcgccccca cccaaggcgg aagccgcagt ccgtccggcc
     2521 gtcgcgcccc gggccgccgc cgtaccgccg cgggccgcgg ccgctcccgc cgcgcccgtc
     2581 cgcgaacagg cgcaagcgca agccgccact gttctccagg cgcaggcggc gagccgcccg
     2641 gccgcgggcg gtgc
//
LOCUS       DBNGWZ010000348         2050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_293364, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000348 DBNGWZ010000000
VERSION     DBNGWZ010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_293364"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16CX_10000"
     CDS             complement(<1..232)
                     /locus_tag="AC16CX_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012878.1"
                     /translation="MNKGMPVGLVLSACMAVAQLGVSPEASGQEAKGLRVVSEQTATG
                     FKFPESVACDAKAKVLYVGQFGSELKPAEKDGK"
     gene            complement(299..868)
                     /locus_tag="AC16CX_10005"
     CDS             complement(299..868)
                     /locus_tag="AC16CX_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3012879.1"
                     /translation="MVEIDHEALRKARLMEDVMFSRAAIGGPFSLTDHHGRRRSLEEF
                     RGKVVMLYFGYTFCPDVCPTDLAAISSMIEALGPSGEKVQPLFVTLDPEHDTVEQLGL
                     YVPHFHPRLIGLTGTLQEIGEVAERYKVYFKKVPAREGPHYYLDHSAHIYLLDGQGKY
                     YGTFPPGTSPKRLASVVWELMSVTPAAAR"
     gene            complement(968..>2050)
                     /locus_tag="AC16CX_10010"
     CDS             complement(968..>2050)
                     /locus_tag="AC16CX_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3012880.1"
                     /translation="GSAATLGSFGLLRHASSVTLAPGAAVPAVDSLSIKVLMDSSHDI
                     FLRAEAPKVVKVRRAPPAADYRKTLHNVWGLSLALESKAGADTRRIMLDFGYTPDTLL
                     NNIELLNVDPARLDALIVSHGHFDHFGGLVGFLDKYRASMKPDLTLYVGGEDNFCTRL
                     RRTPQPGHFAEWGILDRREISARQLNLVLCEKPTVIAGHAFTTGAIRRTGIEKVLPNT
                     MVVRGMKDGLGCNVSLDHFTEAEKLGKPTPDEHIHEHATCYNLKGRGLVVLNSCGHAG
                     ILNSIRQAMEVSGVNKLHALVGGFHLAPAPEDYLKQVVAQLKDLNPDVVIPMHCSGTN
                     FVQEVQRQMPDRLALSTTGSEFTLGA"
BASE COUNT          383 a          675 c          600 g          392 t
ORIGIN      
        1 ccttgccgtc cttttccgcg ggcttcaact cggatccgaa ctgccccacg tacaggacct
       61 tggccttggc gtcgcaggcg accgattcgg gaaacttgaa tcccgtcgcc gtttgctcgg
      121 agaccacccg aagccctttc gcttcctggc cggatgcctc cggagagacc ccgagttgcg
      181 cgacggccat acacgctgac agaaccagac caaccggcat tcccttgttc atggcgccct
      241 cctttccgat gacttctcgc ctcaatcgtc acttcgtcac tgcatcacct catcgcgttc
      301 agcgggcggc ggccggcgtc acgctcatca gttcccacac gacggacgcc agccgctttg
      361 gcgatgtgcc tggcggaaag gtcccgtagt atttgccctg tccgtcaagc aaatagatat
      421 gggcgctgtg atcgagatag taatgcggtc cttcgcgcgc cggcactttt ttgaaataca
      481 ccttgtagcg ctcggcgacc tcaccgattt cctgcaacgt cccggtcagc ccgatcaggc
      541 gcggatgaaa gtgaggaacg tacagaccca gctgctcaac cgtatcgtgt tctggatcga
      601 gcgtgacgaa cagcggttga actttttccc cggacgggcc gagcgcttcg atcatgctcg
      661 aaatcgccgc gagatcggtc gggcagacat cggggcagaa ggtgtagccg aaatacagca
      721 tcacgacctt gccccggaac tcctcgaggc tgcgccgccg accatggtgg tcggtcaatg
      781 aaaaagggcc gccgatcgcg gccctgctga acatgacgtc ttccatgagg cgcgccttgc
      841 ggagcgcctc atggtcgatt tccaccaccg gcttccactg ccacgccgcc atgacgccag
      901 ccacggcggc gagtacggcg acgcacgcaa ttgcaacggt gcgtctgccg gccagtctga
      961 ccacgcgcta cgcgccgagc gtgaactcac tgccggtcgt tgacagcgcg aggcgatccg
     1021 gcatctggcg ctgcacttcc tgcacgaaat tcgtgcccga gcaatgcatc gggatcacga
     1081 cgtccggatt caagtccttt aactgcgcga ctacctgctt gagatagtct tccggtgccg
     1141 gcgcaagatg gaatcccccc accagcgcgt gcagcttgtt caccccggaa acctccatcg
     1201 cctggcgaat cgaattgagg atcccggcgt gcccgcagga attcagcacg acaagccccc
     1261 gccccttgag gttgtagcac gtcgcatgct cgtggatgtg ctcgtccggg gtcggcttgc
     1321 ccaacttctc ggcttcggta aagtgatcga ggctgacgtt gcatcccagc ccgtccttca
     1381 tgccgcgcac gaccatggtg ttcggcagca ccttctcaat ccccgtccgg cgtatcgccc
     1441 cggtagtgaa agcgtggccc gcgatgacag tcggtttctc gcacagcacg agattgagct
     1501 gccgggccga tatctcgcgc cggtcgagaa tgccccactc cgcgaagtgg cccggctgcg
     1561 gcgtgcgtcg cagccgcgtg caaaaattgt cctcaccacc cacgtaaagc gtgaggtcgg
     1621 gtttcatcga ggcgcggtac ttgtcgagaa accccaccag gccgccgaaa tggtcaaagt
     1681 gtccgtgcga cacaatcagc gcatccagcc tcgcggggtc aacgttcagc aactcgatgt
     1741 tattgagcaa cgtatccggc gtgtagccaa agtccagcat gatgcggcgg gtatcggcgc
     1801 ccgccttcga ctccagggcc agcgacagac cccatacgtt gtggagcgtc ttgcggtaat
     1861 cggccgcagg cggggcgcgc ctgactttca ctaccttggg cgcttccgcg cgcaggaaga
     1921 tgtcatggct ggaatccatg agcaccttga tgctgaggct gtccacggcg ggcactgccg
     1981 cgccgggcgc aagcgttact gacgaggcat gcctgagcag gccgaatgaa ccgagagtcg
     2041 ccgcggatcc
//
LOCUS       DBNGWZ010000349         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2308778, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000349 DBNGWZ010000000
VERSION     DBNGWZ010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2308778"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="AC16CX_10015"
     CDS             complement(<1..188)
                     /locus_tag="AC16CX_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012881.1"
                     /translation="MTFSDAQGMRIGVHEPASDTIVDLSAGTRLPRRMTEFIALGKNG
                     LSRARRAVKSGEGRIPAG"
     gene            complement(197..1171)
                     /locus_tag="AC16CX_10020"
     CDS             complement(197..1171)
                     /locus_tag="AC16CX_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012882.1"
                     /translation="MNPLRSASLLVFAVSVLYGACAVAQTYPVKPIRVVVPFGAGGPA
                     DIYARYLGQQLQGPLGQPFVVEDRPGAGSIIGTDIVAKSAPDGYTLLVMSNTHTVNES
                     LVPKKPFVLMKDFVPVVPINYSDLLLVVHPSLPVKSVKELVALAKAKPRGLNYSSSGT
                     GTPYHMAGELFKALAGVDIVHVPHKGSGEARTSVMSGQTEMMLDAITTMLPVARAGRV
                     RPLGTSGRTRSTVAPDLPTIAEAGVKGYETTIWIGIMAPAGTPRAVIEKLNAEITKVT
                     NRPDVKKTWNDQGAQPMTMTVAEFEKYLHADIAKWAKVVKIAGARADQ"
     gene            1381..>2011
                     /locus_tag="AC16CX_10025"
     CDS             1381..>2011
                     /locus_tag="AC16CX_10025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3012883.1"
                     /translation="MIKNKAIKQDRRRFLQAAGTAAVGFVLGALPRALRAAGEPPKGL
                     RIGVIGSGRLGGTVGELWVKAGHQVMFSSLEIEHDRALAARLGSGARAGTPREAAAFG
                     EVLLVAVPYAALPAVGRDLGGLLKDKIVLDASNPIPSRDGDMAIEARAKGTGVASPLF
                     LPGSRLVRAFNSVGYSRLQSEAHRSGERVAIPLAADDAQALQTAARLVRD"
BASE COUNT          321 a          676 c          659 g          355 t
ORIGIN      
        1 cttccggccg ggatgcgccc ctcgccgctc ttcaccgcgc gccgggcgcg ggagaggccg
       61 ttcttgccga gcgcgatgaa ctccgtcatc cggcgcggca gccgcgtgcc ggccgaaaga
      121 tcgacgatgg tatcggaagc cggctcatgc acgccgatgc gcatgccctg cgcgtcggaa
      181 aacgtcacca gtcgcattac tggtcggccc gcgcgccggc gatcttgacc accttcgccc
      241 atttagcaat gtcggcatgc agatacttct cgaactcggc gaccgtcatc gtcatcggtt
      301 gcgcgccctg gtcgttccac gtcttcttca cgtccggccg gttggtaacc ttcgtgatct
      361 cggcattgag tttttcgatc accgcccgcg gcgtcccggc gggcgccatg atcccgatcc
      421 agatggtggt ttcatagcct ttcacgcctg cctccgctat cgtcgggaga tccggcgcca
      481 ccgtggaacg cgtccgtccc gaagtgccca gcggccgcac cctgccggcc cttgcgaccg
      541 gcagcatggt ggtgatcgcg tccagcatca tctcggtctg cccgctcatg acgctggtgc
      601 gcgcttcacc gctgcccttg tgggggacgt gcacgatgtc cacgccggcc agcgccttga
      661 acagctcccc cgccatgtgg tacggcgtgc cggtgcccga ggatgagtaa ttgagcccgc
      721 gcggcttcgc tttcgcgagc gcgaccagtt ctttcaccga cttgaccggg agcgagggat
      781 gcaccaccag cagcaggtcc gagtagttga tcgggaccac cggcacgaag tccttcatca
      841 gcacgaaggg cttcttcggg accagcgact cgttgaccgt atgggtgttg gacatgacca
      901 gcagcgtgta gccgtcgggg gccgatttcg ccacgatgtc ggtgccgatg atcgagcctg
      961 cgccggggcg atcctcgacg acgaacggct gccccagggg accctgcagc tgctggccga
     1021 ggtagcgggc gtagatgtcg gccggccctc ccgcgccgaa tggaacgacg acgcgtatcg
     1081 gtttcacggg ataggtctgc gcgaccgcgc aagcgccgta caaaacggat accgcaaata
     1141 cgagcagtga tgccgagcgc aacggattca tgtaccctcc tgctggattg ccggatgacg
     1201 tcccgttgta aatgttatcg agacgccgca gcgggcagta tccaatcccg cgttgtctgc
     1261 gtcaaccgcg gccggctgtt tgctatattg gcgcgccgaa aggacgccgc ggagtccgct
     1321 cgatcaaggt cgattccatt cgggcgccat ggacgaacgt tcacgccaag gagatcgctc
     1381 atgataaaga acaaggccat caagcaagac cgccgcagat ttctccaggc cgcgggaact
     1441 gccgcggtcg gtttcgtcct gggcgcgctg ccgcgcgcgc tgcgtgccgc gggcgagccg
     1501 cccaagggcc tcagaatcgg agtgatcggc tcgggcagac tcggcggcac ggtcggcgag
     1561 ctgtgggtga aggccggcca ccaggtcatg ttttcatcgc tcgaaatcga gcacgacagg
     1621 gcgctcgcgg cgcgcctggg ttccggcgcg cgcgccggca cgccgaggga agccgcagcg
     1681 ttcggtgaag tcctgctggt tgccgttcct tatgcggcgc tcccggcggt cgggcgcgat
     1741 ctcggcggcc tcctcaagga caagatcgtg ctcgacgcca gcaatccgat accgtcgcgc
     1801 gacggcgaca tggcgatcga ggcaagggcc aagggcaccg gcgtcgcgtc tccgctgttc
     1861 ctgccgggct cccgcctggt gcgagccttc aattccgtcg gctactcgcg cctgcaaagc
     1921 gaagcgcacc gctccggcga gcgggtcgct attccactcg ccgccgacga tgcgcaggcg
     1981 ctgcagaccg cggcgcgcct cgtgcgggac g
//
LOCUS       DBNGWZ010000350         9984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_794797, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000350 DBNGWZ010000000
VERSION     DBNGWZ010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_794797"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="cyoE"
                     /locus_tag="AC16CX_10030"
     CDS             <1..743
                     /gene="cyoE"
                     /locus_tag="AC16CX_10030"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3012884.1"
                     /translation="TLGIWLVAGAAAAINCLVEQRLDAVMTRTRGRPLPRGELTATQT
                     LAFAGIMGGAGLWLLYALVNPLTMWLTLGTFVGYAVIYTILLKPMTPQNIVIGGASGA
                     MPPVLGWAAVTGEIASDALLLFLIIFAWTPPHFWALALYRKREYAKAGVPMLPVTHGD
                     RFTRLHVLLYTVILTAVTLMPFATRMSGAIYLAAALVLDAVFLRHAVRIYFAYSDRLA
                     QRTFRYSIFYLTALFAALLLDHYFRVPL"
     gene            768..1358
                     /locus_tag="AC16CX_10035"
     CDS             768..1358
                     /locus_tag="AC16CX_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3012885.1"
                     /translation="MKRLATTVLSVFAAAWLAGCGGGMDTPKFRLTDVTGAGIGKELN
                     LTDHNGRLRTLADFRGKVVTVFFGFTHCPDACPTTLAEMAQVVRELGPDGGKVQVLFV
                     TVDPERDTPEVLGKYVPSFHPSFLGLYGDAEATAHAAKEFKVYYQKQPAKGGYSVDHS
                     AGTFILDREGRVRLFAQYGQGPAAILHDVRLLLAGA"
     gene            complement(1407..2258)
                     /gene="rpoH"
                     /locus_tag="AC16CX_10040"
     CDS             complement(1407..2258)
                     /gene="rpoH"
                     /locus_tag="AC16CX_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HIR3012886.1"
                     /translation="MDFALPVPSAAGSLETYIQAVNRFPILTREQETALARRYRNEGD
                     LEAARELVLSHLRVVVAIARGYLGYGLPQADLIQEGNIGLMKAVKRYDPERGVRLVSF
                     AVHWIRAEIHEFVLRNWRIVKIATTKAQRKLFFNLRSLKQNLASLGTEETKAVARQLG
                     VKPEEVVEMEMRLAGQDVALEPAGDDEEESYAPIAYLAAEDAEPGRILEHEESARLHS
                     AGLERALESLDARSRRIVEARWLNEKEPATLHDLAAEFGVSAERIRQIEAKALSRMKG
                     VITRQAA"
     gene            complement(2430..3338)
                     /gene="ftsX"
                     /locus_tag="AC16CX_10045"
     CDS             complement(2430..3338)
                     /gene="ftsX"
                     /locus_tag="AC16CX_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR3012887.1"
                     /translation="MRTWLWHHIDAFGTTFARLARTPVATLLNVGVIGIALALPVGLY
                     VALVNLQGLANSLASDPQLTAFLALDASRADVKQIETRLKQHAGVREARFVPREQALT
                     ELKAATGLADVVDSLPQNPLPDAFVLVPRETAPQNLEKLRDEVSSWPKVTHVQLDTAW
                     AQRLEAGLRLARLAVWLLATLFAFALVAVTFNTIRLQVLTRREEIEVSKLIGATDPFI
                     RRPFLYFGTLQGLAGGLAAWIIIWAGIQLLNGALSELAQLYAARFELRHLSPADSLSL
                     FVFSAGLGWFGSWLSVNRHLAEIEPQ"
     gene            complement(3335..3988)
                     /gene="ftsE"
                     /locus_tag="AC16CX_10050"
     CDS             complement(3335..3988)
                     /gene="ftsE"
                     /locus_tag="AC16CX_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR3012888.1"
                     /translation="MISFSHVTKRYPGGQEALRGITLAIKAGEMVFITGHSGAGKSTL
                     LKLAAAIERPTGGSVIINGQNVGALRARAVPYLRRNFGLVFQDQKLLFDRTVFDNVML
                     PLAITGFERREAARRVRAALDKVGLLAREKSFPVTLSGGEQQRLAIARAIVHRPAVLI
                     ADEPTGNLDSVHAAEIGELFRSFNQVGVTVVVATHDEAFAAQFRPRLVALREGRLAA"
     gene            complement(4082..5029)
                     /gene="ftsY"
                     /locus_tag="AC16CX_10055"
     CDS             complement(4082..5029)
                     /gene="ftsY"
                     /locus_tag="AC16CX_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3012889.1"
                     /translation="MLSFLKTSPENGAGGWFARLRNGLTKTREQLGNRLAGLFGASRP
                     LDESFYEELETALIMADVGVAASAHLLGELRRRTRHEGIPDSTRLKQALQAALRELLE
                     PVAVPLEVEAQRPFVIMLAGVNGSGKTTSIGKLAHYYQSRGKSVLLAAGDTFRAAARE
                     QLIAWGERNNVTVITQQSGDAAAVIYDAVNAAVARGIDVVLADTAGRLPTQLHLMEEI
                     KKVKRVIAKALPGAPHEVLLVLDANTGQNALAQVRAFDDALGVTGLVLTKLDGTARGG
                     VIAAIARERPIPVRFIGVGEGLDDLRPFDAADFVAALFD"
     gene            5150..6529
                     /locus_tag="AC16CX_10060"
     CDS             5150..6529
                     /locus_tag="AC16CX_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3012890.1"
                     /translation="MKFPGRLFCALLAVMIGATPGMAATDTAERTLANGMKVVVKPDH
                     RAPVAVAVVWYKVGSVDEVSGVTGVAHVLEHMMFKGTQTIAPGQYSRTIAVAGGRDNA
                     FTSNDFTGYFATLHKSQLELMLRLEADRMANLTIPPEEFAKEIKVVMEERRYRTDDRP
                     RSLTYEQLMATALTAHPYRNPIIGWMNDLENMRVEDVREFYHRWYAPNNAILTVVGDV
                     DPKEVFALAEKHFGAISPRELPVRKPQDEPPQLGEKRVTVKAPAELPYVLIGFRAPAL
                     RDAEKDWEPYALRMLAAVLDGNEAARLPRALVREQRLASAADASYDGIARGPDFFYLS
                     ATPTPGKSAAEVEQGLRREMARIINEGVTEEELNRAKAQAIASEVYQRDSMFFQARMI
                     GVLEATGLSHRTIDLQLKKLREVTPEQVREVARKYFRDDALTIAHLDPQPLEGRRPTA
                     PPAGLRHVQ"
     gene            6519..7847
                     /locus_tag="AC16CX_10065"
     CDS             6519..7847
                     /locus_tag="AC16CX_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3012891.1"
                     /translation="MCSKCISIGFVAAALTAAALPAQAILPIQHWQTPSGARVYFVEN
                     HDLPMLDVSVEFPAGSGYDRPEKTGVAAMTNRLLNTGADGMSEDDIARRIADVGAQLG
                     GRFDTDRAGMALRTLSSASERRQALDILARVLRRPVFPQDVLEREKVSLIGALKEADT
                     KPDTIASRAFYRLVYRDHPYALRATGEVETVEKIAREDLVDFYRRHYTSQHTVVALIG
                     DVTRAEAETIAEQVTRGLPAADGPEPLLPPVSELPAGTTRMIGHPALQAHILIGAPGI
                     RRDDPDYFPLFIGNYVLGGGGFVSRITEEVRQKRGLAYSAYSYFSPLRREGPFLIGMQ
                     TRRDQAGQALEVARTTLIDFVRDGPTEQELTAAKQNIIGGFPLRIDSNRKIHEYLALI
                     GFHRLPLTYLEDFVKNVERVTAAEIREAFQRRIHPDRMVTVVVGGDETKP"
     gene            7940..8497
                     /gene="rsmD"
                     /locus_tag="AC16CX_10070"
     CDS             7940..8497
                     /gene="rsmD"
                     /locus_tag="AC16CX_10070"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3012892.1"
                     /translation="MNRNRVRIIGGAWRSRLISFPARKDLRPTPDRVRETLFNWLGQD
                     LTGKSCLDLFAGSGALGFEAASRGARRVVMVESDATVFRALAASRAALNAASVELRKA
                     DALEFLRADADVYDVVFLDPPFRTDYWPRLSALLPPRLAPGALVYYEGAAPPDLPPGW
                     EVWRQDRAGQVRYQLLKPGMELLTR"
     gene            8877..9371
                     /gene="coaD"
                     /locus_tag="AC16CX_10075"
                     /pseudo
     CDS             8877..9371
                     /gene="coaD"
                     /locus_tag="AC16CX_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903422.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
     gene            9485..9739
                     /locus_tag="AC16CX_10080"
     CDS             9485..9739
                     /locus_tag="AC16CX_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013337947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR3012893.1"
                     /translation="MALKITDECINCDVCEPECPNDAISQGEEIYVIDPHRCTECVGH
                     FDEPQCRKVCPVDCIPLDPDFVETKEQLFAKYLKLTTEKA"
     gene            complement(9821..>9984)
                     /locus_tag="AC16CX_10085"
     CDS             complement(9821..>9984)
                     /locus_tag="AC16CX_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HIR3012894.1"
                     /translation="RDYVRSDGLAGNFQSQFLVYDRAGEPCRRCGTLIKGIRQGQRST
                     FYCPCCQRR"
BASE COUNT         1737 a         3236 c         3323 g         1688 t
ORIGIN      
        1 cgacgctggg gatctggctg gtggccggcg ccgcggcggc gatcaactgc ctggtggaac
       61 agagactcga cgccgtgatg acccgtacgc gcggacggcc gctgccgcgc ggagagctca
      121 ccgccacgca gacgctcgcg ttcgcgggca tcatgggcgg ggcgggcctt tggctgctct
      181 acgcgctcgt gaacccgctc acgatgtggc tgaccctcgg cacgttcgtc ggctacgccg
      241 tgatctatac gatcctgctc aagccgatga cgccgcagaa catcgtgatc ggcggcgcct
      301 cgggcgcgat gccgccggtg ctgggctggg ccgcggtgac cggcgagatc gcgtccgacg
      361 cgctgctgct gttcctgatc attttcgcgt ggacgccacc gcacttctgg gcgcttgcgc
      421 tctaccgcaa gcgcgaatac gcaaaagccg gcgtgccaat gctgccggtg acgcacggcg
      481 accggttcac gcgcctgcac gtgctgctct acaccgtgat cctcacggcg gtgacgctga
      541 tgccgttcgc cacccgcatg agcggcgcga tctacctcgc cgcggcgctg gtgctcgatg
      601 ccgtgtttct gcggcacgcg gtgcgcatct acttcgccta cagcgaccgt ctcgcgcaac
      661 gcaccttccg ctattccatc ttctacctca cggcgctgtt cgcggcgctg ctcctcgatc
      721 attatttccg cgtcccgctc tgaaagcggg cggcgcgggt gtctcgcttg aagcggctcg
      781 ctacgacggt tctttctgtt ttcgccgctg cctggcttgc cggatgcggc ggcggcatgg
      841 acacgcccaa attcaggctg accgacgtga cgggggcggg tatcggaaaa gaactcaacc
      901 tgaccgatca caatggtagg ctgcgcacgc tcgcggattt ccgcggcaag gtggtgacgg
      961 tgtttttcgg cttcacccac tgtcccgacg cgtgcccgac gacgctggcg gagatggcgc
     1021 aggtcgtcag ggagctcggc ccggacggcg gcaaggtgca ggtgttgttc gtgacggtgg
     1081 acccggaacg cgacaccccc gaagtgctcg ggaaatacgt gccgtcgttc catccgtcgt
     1141 ttctcgggct ctacggcgac gcggaggcaa ccgcgcacgc ggcgaaggaa ttcaaggtct
     1201 actaccagaa gcagccggcc aagggcggct acagcgtgga ccactccgcc ggcacgttca
     1261 ttctcgaccg tgaaggtcgg gtgaggctgt tcgcgcagta cgggcagggc ccggccgcca
     1321 tcttgcacga cgtccggctg cttctcgcgg gcgcataaaa aaagccccga aacacccggg
     1381 gcttttttaa agtggttcct gaaaggttac gccgcctggc gggtgatgac gcccttcatc
     1441 ctggagagcg ccttcgcttc gatctggcgg atgcgctcgg ccgagacgcc gaattcggcc
     1501 gcgagatcgt gcagtgtcgc cggctccttc tcgttcagcc agcgcgcctc gacgatgcgg
     1561 cggctgcgtg catccaggct ctccagcgcc ctctccagcc ccgcgctgtg caggcgcgcg
     1621 ctctcctcgt gctcgaggat acggccgggc tcggcatcct cggccgccag gtaagcgatc
     1681 ggggcatagc tttcttcctc gtcgtcgccc gccggctcga gcgcgacgtc ctggccggcg
     1741 agccgcattt ccatctcgac cacttcctcg ggcttgacgc cgagctgacg cgcgaccgcc
     1801 ttcgtctcct cggtgccgag gctcgcgagg ttttgcttga ggctcctcag gttgaagaac
     1861 agcttcctct gcgccttggt ggtcgcgatc ttcacgatgc gccagttgcg caggacgaat
     1921 tcatggattt cggcgcggat ccagtgcacg gcgaacgaca ccagccgcac gccgcgctcg
     1981 ggatcgtagc gcttgaccgc cttcatgagc ccgatgttgc cttcctggat gaggtcggcc
     2041 tgcggcaggc cgtaaccgag gtagccgcgc gcgatcgcga ccaccacccg caggtgcgag
     2101 agcacgagct cgcgtgccgc ttcgaggtcg ccctcgttgc ggtagcggcg ggcgagcgcc
     2161 gtctcctgct cccgggtcag gatcgggaag cggttgacgg cctggatgta ggtttccagg
     2221 ctgccggcag ccgatgggac gggcaacgca aaatccatcg tttaaccccc tttgctgaac
     2281 tattttagca ctcaaggtct gagagtgcca gaagctatga aagttccttc aattcatgga
     2341 gttgggattg aatatccgga tcaaaatccg cttcaaatca gaagcttgga cgcagtcagg
     2401 agcttgcccg gccgaggccc ggcaagatcc tactggggct cgatttcggc caggtgccgg
     2461 ttgaccgaga gccaggagcc aaaccagccg agaccagccg aaaacacgaa caggctcagg
     2521 ctgtccgcgg ggctcaggtg ccgcaattcg aagcgcgcgg catagagttg cgcgagttcg
     2581 gacagcgcgc cgttcaggag ctgaatgccg gcccagatga tgatccatgc ggcaaggccg
     2641 ccggccagcc cctgcagcgt cccgaagtag aggaagggac gccggatgaa ggggtcggtc
     2701 gcgccgatca atttcgatac ttcgatctcc tcgcggcggg tgagaacctg caggcgaatc
     2761 gtgttgaagg tcacggccac cagcgcaaag gcgaacagcg tcgcgagcag ccatactgcc
     2821 agccgcgcga gcctcaggcc cgcctccagg cgctgcgccc aggctgtgtc gagctgcacg
     2881 tgcgtgacct tgggccagct gctcacttcg tcgcgcagct tctccaggtt ctgcggtgcc
     2941 gtctcccgcg gcaccagcac gaacgcatcc ggcaacggat tctggggcag gctatccacg
     3001 acgtcggcga gcccggtggc cgccttgagt tccgtcaacg cctgctcgcg cggcacgaag
     3061 cgcgcttcgc gcacccccgc gtgctgtttc agccgggtct cgatctgctt cacgtcggcg
     3121 cggctggcat ccagcgcgag gaaagccgtg agctggggat cggaagcgag cgagttcgcc
     3181 aggccctgca agttgaccag agccacgtaa agcccgaccg gcagcgcgag cgcgatgccg
     3241 atcaccccca cattgagcag ggtcgccacc ggcgtgcgcg caagccgcgc aaatgtcgtg
     3301 ccgaaggcgt caatgtggtg ccacaaccag gttctcatgc cgcgagcctc ccctccctga
     3361 gcgcgaccag gcggggcctg aactgcgcgg cgaacgcctc gtcatgggtc gcgaccacca
     3421 cggtgacccc gacctggttg aacgaccgga acagctcgcc gatctcggcg gcgtggacgg
     3481 agtcgaggtt gccggtcggt tcgtcggcga tcagcacggc ggggcggtgc acgatggcgc
     3541 gcgcgatcgc gagccgctgc tgctcgccgc ccgagagcgt gaccggaaag gacttctccc
     3601 gggcgagcag gccgaccttg tcgagcgccg cgcgcacgcg ccgcgccgcc tcgcgccgct
     3661 cgaatccggt gatggcgagc ggcagcatca cgttgtcgaa gacggtgcgg tcgaacagca
     3721 gcttctggtc ctggaagacg agaccgaaat tgcggcgcag gtacggcacg gcgcgcgccc
     3781 gcaacgcccc cacgttctgg ccgttgatga tcacgctgcc gccggtcggc cgctcgatcg
     3841 ccgccgcgag cttgagcagc gtgctcttac cggccccgga gtggccggtg atgaacacca
     3901 tctcgccggc cttgatcgcg agcgtgatac ctctcaacgc ctcctgtccg ccggggtagc
     3961 gcttggtgac atggctgaag gaaatcatgc ggttaggtag ggcagctggg cgttagtagg
     4021 cactcactgg gggaggcggt acggccggtt tcggccattt cgtggtggaa gccgctccgc
     4081 atcagtcgaa tagcgcggcg acgaaatccg cggcatcgaa gggccgcagg tcgtccagtc
     4141 cctcgccgac gccgatgaag cgcacgggta tcggccgctc ccgcgcaatg gcggcgatga
     4201 caccgcccct ggcggtgccg tcgagcttgg tcagcacgag accagtcacg cccagggcgt
     4261 cgtcaaacgc ccgcacctgc gcgagcgcgt tctggccggt gttggcatcc agcaccagca
     4321 gcacctcatg gggcgcgccc gggagcgcct tggcgatcac gcgcttgacc ttcttgatct
     4381 cctccatgag gtgcaactgg gtggggagcc gccccgcggt gtcggccagc acgacatcga
     4441 tgccgcgggc gaccgcggca ttgaccgcgt cgtagatcac cgccgccgca tcgcccgact
     4501 gctgcgtgat cacggtcacg ttgttgcgtt cgccccacgc gatcaactgc tcgcgcgccg
     4561 ccgcgcggaa agtgtcaccc gcggcaagca gcacggattt gccgcggctt tggtaatagt
     4621 gcgcgagctt gccgatcgag gtggtcttgc cgctgccgtt gacgcccgcg agcatgatca
     4681 cgaacggccg ctgcgcctcc acctcgaggg gaaccgcgac cggctccagc agctcgcgca
     4741 acgccgcctg cagcgcctgc ttgagacgcg tcgaatcggg gatgccttcg tggcgggtgc
     4801 gccgccgcaa ctcgccgagc agatgagcgc tcgccgccac tccgacgtca gccatgatca
     4861 gcgctgtttc cagctcttcg tagaacgact cgtccagcgg tcgcgacgcg ccgaacaacc
     4921 cggcgagacg gttgcccagc tgctcgcggg tcttggtgag gccgttccgg aggcgcgcaa
     4981 accagccgcc ggcaccgttt tccggggatg tcttgaggaa acttagcacg cgcagagcag
     5041 tctacaggcg gatgggcgat tggcgggcga gatcgaaaag tcgaatatcc agcccgcgga
     5101 attggataca attctagccc gaaatccact gcggccgaag cctggactga tgaaattccc
     5161 tgggcgtctc ttttgcgcgt tgcttgccgt gatgatcggc gcaacgcccg gcatggcggc
     5221 caccgatacc gccgagcgca cgctcgcgaa cggcatgaag gtggtcgtca agcccgacca
     5281 ccgcgcgccg gtggcggtgg cggtggtgtg gtacaaggtg ggcagcgtgg atgaggtcag
     5341 cggcgtcacc ggcgtcgcgc acgtgctcga gcacatgatg ttcaagggca cccagaccat
     5401 cgcgcccggg cagtactcgc gcaccatcgc cgtggcgggc gggcgcgaca atgccttcac
     5461 cagcaacgac ttcacgggtt atttcgccac gctgcacaaa tcgcagctcg agctcatgct
     5521 gcggctggag gccgatcgca tggccaatct caccatcccg cccgaagagt tcgccaagga
     5581 gatcaaggtg gtgatggagg agcggcgcta ccgcaccgac gaccgcccgc gctcgctgac
     5641 ctacgagcaa ttgatggcga ccgcactcac ggcgcatccc tatcgcaacc cgatcatcgg
     5701 ctggatgaac gatctcgaga acatgcgggt cgaggacgtg cgcgagttct accaccggtg
     5761 gtacgcgccc aacaatgcga tattgacggt ggtgggggat gtggacccga aggaggtgtt
     5821 cgccctcgcg gagaagcatt tcggggcgat ttcgccgcgt gaactgccgg tgcgcaaacc
     5881 gcaggacgag ccgccacagc tgggcgagaa gcgggtgacg gtcaaggccc ccgccgagct
     5941 gccatacgta ttgatcggat tccgcgcgcc ggcgttgcgc gatgccgaga aggactggga
     6001 accgtacgcg ctgagaatgc tggccgcggt gctcgacgga aacgaggcgg cgcggctccc
     6061 gcgcgcgctg gtgagggagc agcgcctggc gagcgccgcg gacgcgagct atgacggcat
     6121 cgcgcgcggc ccggattttt tctacctcag cgcgacgccg acgccgggca agagcgccgc
     6181 ggaagtcgag cagggcctga gacgcgaaat ggccaggatc atcaacgagg gcgtgaccga
     6241 ggaggagctc aaccgcgcga aggcccaggc gatcgcctcc gaggtctacc agcgcgactc
     6301 gatgttcttc caggcgcgca tgatcggcgt gctggaagcc accggcttgt cgcaccggac
     6361 gatcgacctg cagctcaaaa aactgcggga agtcacgccc gagcaggtgc gcgaggttgc
     6421 ccggaaatac ttccgggacg atgccttgac catcgcccat ctcgacccgc agccgctcga
     6481 gggacggcgg ccgacagcgc ctccagcggg acttcgccat gtgcagtaaa tgcatctcga
     6541 tcgggtttgt tgcggcggca ttgaccgccg cggccctgcc ggcgcaggcg atcctgccga
     6601 tacagcactg gcagaccccg agcggggcgc gcgtctactt cgtcgagaac cacgacctgc
     6661 cgatgctgga tgtcagcgtg gaattccccg cggggtcggg ttacgaccgg cccgagaaga
     6721 caggcgtagc tgccatgacc aaccgcctgc tcaacactgg cgcggacggc atgagcgagg
     6781 atgacatcgc ccggcgcatt gccgatgtcg gcgcgcagct tgggggacgc ttcgacaccg
     6841 accgcgcggg gatggcgctg cgcacgctgt ccagcgcgag tgagcgccgc caggcgctcg
     6901 acatcctcgc gcgcgtgctg cggcgcccgg ttttcccgca ggatgtgttg gagcgcgaaa
     6961 aggtgagcct gatcggggcg ctgaaggaag cggataccaa acccgacacc atcgcctcgc
     7021 gcgccttcta tcgcctggtc taccgcgacc acccgtatgc gctgcgcgcc acgggcgaag
     7081 tggaaacggt cgagaaaatc gcgcgcgagg acctggtcga tttctaccgg cgccattaca
     7141 cctcgcagca cacggtcgtc gcgctgatcg gggacgtgac gcgcgctgag gccgagacga
     7201 tcgcggagca ggtgactcgc ggattgccgg cggcggacgg cccggaaccg ctcctgcccc
     7261 cggtcagcga gcttccggcg ggcacgacgc gcatgatcgg gcaccctgcc ttgcaggcgc
     7321 acatcctgat cggcgcgccc ggcatccgcc gcgacgatcc ggattacttt ccgctgttca
     7381 tcggcaacta cgtgctgggc ggcggcggct tcgtgtcacg catcaccgag gaggttcgcc
     7441 agaaacgcgg cctcgcttat tcggcttaca gctatttctc gccgctcagg cgcgagggcc
     7501 cgttcctgat cggcatgcag acccggcgcg accaggccgg gcaggcactc gaggtggccc
     7561 ggaccaccct gatcgatttc gtgcgcgacg gcccgaccga gcaggagctg acggcggcca
     7621 agcagaatat catcgggggt tttccgctgc gcatcgacag caaccgcaag atccacgagt
     7681 acctcgccct gatcggcttc caccgcctgc cgctcactta cctggaagac ttcgtgaaga
     7741 acgtcgagcg cgtcaccgcc gccgaaatcc gcgaggcctt ccagcggcgg attcaccccg
     7801 accgcatggt gaccgtggtg gtcggcggcg acgaaaccaa accttgagaa acggacggga
     7861 gggcgtgagc gcgcgagagc gggagagcga aaagagccac cttccatctc ccgatttcac
     7921 gatctcccgg cttttggtaa tgaaccgcaa ccgggtgcgg atcatcggcg gcgcgtggcg
     7981 cagccggctg atctcgttcc cggcgaggaa ggacctgcgt cccacgcccg accgcgtgcg
     8041 cgagacgctg ttcaactggc tcgggcagga tctcacgggt aagtcctgcc tcgacctctt
     8101 cgccggcagc ggcgccctgg gattcgaggc cgcctcgcgc ggggcgcggc gcgtggtgat
     8161 ggtggaaagc gacgcaacgg tattccgcgc gctcgccgcg agccgcgcgg cgctcaacgc
     8221 cgcctcggtg gaactgagga aagcggatgc gctggaattc ctgcgcgcgg acgccgacgt
     8281 ttatgacgtc gtctttctcg acccgccgtt taggaccgat tactggccgc gcctgtccgc
     8341 gctgctgcca ccgcgcctcg cgccgggcgc attggtctat tatgagggtg cggcaccgcc
     8401 cgatctgccg ccgggatggg aagtatggag gcaagaccgg gcgggacagg tcagatatca
     8461 actattgaaa ccggggatgg agctcctaac acggtgaaac gcgcgtgcgt tgcgaggcag
     8521 aattggcgat ggcgcgaagc gaggtgcagc caatatgtcg atattagcaa gccgagcgac
     8581 aaaggtcccg cggagggcgg agcaggttcc aagcgatgtg tgcttggatg cgaccccgta
     8641 gcgggacatg agcgcgcaat aaccgcgggc cagtgcacgc cttcacggtc gcatcccgca
     8701 agcgggtccc tgctccacgt tccggcgcgt cggcgattct ggccgcaacc cttgtggctg
     8761 gcgcggtttt ggctcgtggc ttatgctcaa tgcgttcctt atgaaatcta cgcgctttac
     8821 atgcggccaa aaccgtgaca gcgcagcgtg tgtttcatcg tgttaggagc tccttaatga
     8881 accataaagc ggtctacccg ggaacgttcg atccgatcac gctcggccac gaggacctga
     8941 tccggcgcgc ggcgcggctg ttcgacacgg tgatcgtggc ggtggccgac tcgagcgcca
     9001 agcggccgtt ctttacccgc gacgagcgcg tggacatggc gcgccgggtg ctgaaagacg
     9061 ccaagaacgt ggaggtcgtc ggcttctccg gtctgctgat gaagttcatc cggcagcaga
     9121 aagctcgggt ggtgatccgg gggctgcgcg cggtgtcgga cttcgagcat gagttccagc
     9181 tcgccggcat gaaccgcggc atgtatcccg atgtcgaaac cttgttcctg acgcccgggg
     9241 agtagtacat gttcgtttcc gcgaccatcg tgcgtgagat ctcgattctc ggcggcgacg
     9301 tgagaaagtt cgtgccgccg gtggtcgcgc ggcggctcaa ggccaagatc aagcagctcg
     9361 gcggcaagtg acagttgaga acctcgacgc aaagacgcga aggcgcaaag gaaacctgga
     9421 ttaaagttga tggaatgaat actttgcggc tttgcgactt tgcgtctagg gtttgggtgc
     9481 agagatggcg ctgaagatca ccgacgaatg catcaactgc gacgtgtgcg agccggagtg
     9541 cccgaacgac gcgatttcgc agggcgagga gatctacgtc atcgatcccc accgctgcac
     9601 cgagtgcgtc gggcatttcg acgagccgca gtgtcgcaag gtgtgcccgg tggactgcat
     9661 tcccctcgac ccggacttcg tggagacgaa ggagcagctg ttcgcaaagt acctcaagct
     9721 caccaccgaa aaggcgtgaa tcgtgagtgg tgagtggacc aaaccgtaac ctcagtccaa
     9781 tcactattca ctaatcacca ctcacgcttt ttgactgctg tcagcggcgc tgacagcagg
     9841 gacagtagaa cgtcgagcgc tgcccctgtc ggatcccctt gatcaacgtc ccgcagcgcc
     9901 ggcacggctc gccagcgcgg tcgtagacga ggaactggct ctggaagttg ccggcgagcc
     9961 cgtcgctgcg cacatagtcc ctca
//
LOCUS       DBNGWZ010000351         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1928776, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000351 DBNGWZ010000000
VERSION     DBNGWZ010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1928776"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16CX_10090"
     CDS             complement(<1..202)
                     /locus_tag="AC16CX_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3012895.1"
                     /translation="MAKVTIKVNGKKETRAVEDRTLLVNFLRDELGLTGTHVGCDTSQ
                     CGCCTVHVNGRAVKSCTMLAAQA"
     gene            complement(265..1071)
                     /locus_tag="AC16CX_10095"
     CDS             complement(265..1071)
                     /locus_tag="AC16CX_10095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3012896.1"
                     /translation="MYALNYVKAKSVNEAAEFLAANPEAKLLAGGMTLIPTLKQRLAR
                     PTHLVDIAQLAELRKIEAKGGKLVIGAGAKHHEVATSAIVKRAIPALAGLAGEIGDPQ
                     VRNQGTIGGSVANNDPAADYPAVVLGLAAAVVTTKRTIAADDFFQGLFTTALEEGEII
                     VRIEFPVPKRAAYAKFPHPASGYAMAGVFIAQTAGGVRVAVTGAGPGVFRWQEAEVAL
                     AKEGLKPAALDGAKLDASGLNSDIHADREYRANLVRVMAKRALAKLSGRK"
     gene            complement(1219..>3276)
                     /locus_tag="AC16CX_10100"
     CDS             complement(1219..>3276)
                     /locus_tag="AC16CX_10100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3012897.1"
                     /translation="MHTLTFPGKGGMKTRAPARPALAHRKVRFVGEALAMVVADTAAA
                     AQDAAELVEVEYRDLPPAVHPEEALAPGAPQLHEDVPGNLALEAEAGDAAAVESAFAR
                     AAHVTRLKVEVTRVAPNPMEPRAYVAAYDARSDSYTLHVCCQGITTLRRHLSAYTGVP
                     EEKLNFEVRDVGGGFGQRTPAYPEHCALMLAAKETGRPVKWVSTRSEGIACDTHGRAN
                     IIDGALALDGDGRFLAMRLDWVNDMGAYLSPASMGHIRNTSTCMTGVYRIPALYAHYR
                     VALTNTNPVSSYRGAGRPDIAYTVERLVSRAAAELKMDAAELRRRNFIPPEAFPYKTP
                     TGSTYENADLPGVFEKALKLADWKGYPKRRARAEKGGKLRGIGIATVIENTGAGQAAK
                     DEVELTVDAAGNVVAYTMAKAQGHGHETTFAMIVADALGIPLERVSIRQCAPGKQHEL
                     QGNHTGGSRSTVGAGSVCHLAARKLIEHGRALAALELGVEPSQVEYARGVFHTRESKR
                     KLRLGDLAKNGSMNVVAEGKFGSTFPNGCHIAEVEIDPDTGKAEIASYCAVDDIGTVI
                     NHAIVEGQLHGGVAMGAGQIFGEHVVYDRGSGQLLTGSFMDYYMPRAGLLPAIKGAEH
                     PTRSQVSPLGVKGVGESGCTGSLPAIANAVMDALKPLGIGHLDMPLTPAKLWHAIHTA
                     KRG"
BASE COUNT          454 a         1156 c         1069 g          597 t
ORIGIN      
        1 tcgcctgcgc cgcgagcatg gtgcacgact tcaccgcccg gccgttgacg tgcacggtgc
       61 agcagccgca ctggctggtg tcgcagccga cgtgggtgcc ggtgagcccc agctcgtcgc
      121 gcaggaagtt gacgagcagc gtgcggtcct cgaccgcgcg cgtttccttc ttgccgttga
      181 ccttgatcgt taccttggcc atgttctgtt cctggagtga ttcgtgggtc gtgggtcgtg
      241 gatcgagaac gctcccaacc cctgctattt cctgccggag agcttcgcga gcgcgcgctt
      301 cgccatcacc cgcaccaggt tcgcgcggta ctcgcggtcc gcgtggatgt cgctgttcaa
      361 tccggaggcg tcgagcttcg cgccgtcgag cgcggcgggc ttgaggccct ccttcgcgag
      421 cgcgacttcc gcttcctgcc aacggaacac gccgggaccc gcgccggtca ccgcgacccg
      481 cacgccaccc gccgtctgcg cgatgaacac gccggccatc gcgtagcccg aggccggatg
      541 ggggaacttg gcgtaggcgg cgcgtttcgg aaccggaaac tcgatgcgca cgatgatctc
      601 gccctcctcg agcgcggtcg tgaacaggcc ctggaagaaa tcgtccgccg cgatcgtacg
      661 cttcgtcgtg accacggcgg cggcgagacc gagcaccacg gccggatagt cggccgcggg
      721 atcgttgttc gccaccgagc cgccgatcgt gccctggttc cgcacctggg gatcgccgat
      781 ctcgccggcc aggcccgcga gcgccgggat cgccctcttc acgatggcgg aagtggcgac
      841 ctcgtgatgc ttcgcccccg cgccgatcac gagcttgccg cccttcgcct cgatcttgcg
      901 cagctcggcg agctgcgcga tgtccaccag atgcgtcggg cgcgcgagcc gctgcttgag
      961 cgtcgggatc agcgtcatgc cgcccgcgag cagtttcgct tccgggttgg cagccaggaa
     1021 ctccgccgcc tcgtttaccg acttcgcctt tacgtaattc agcgcataca tccgttcgtc
     1081 tcccttccta attctgcgat ccccaccacc cgcctgcgga atcatcccac gtcttgatgg
     1141 acatgagcag cgagcggtaa gccgtgagca gtcgattcca gcgcaacact tttcccctca
     1201 ccgctcaccg ctaacggctt accctctctt cgcagtatgg atcgcgtgcc agagcttcgc
     1261 cggggtcagc ggcatgtcga ggtgcccgat gccgagcggc ttgagcgcgt ccatcaccgc
     1321 gttggcgatc gcgggcagcg agccggtgca acccgactcg cccacgccct tgactccgag
     1381 cgggctcacc tggctccgcg tcggatgctc cgcgcctttg atggcgggca gcagccccgc
     1441 gcgcggcatg taatagtcca tgaagcttcc ggtgagcagc tgcccgctgc cgcggtcgta
     1501 gaccacgtgc tcgccgaata tctggcccgc gcccatcgcg actccgccgt ggagctggcc
     1561 ctcgacgatg gcgtggttga tcaccgttcc gatgtcgtcc accgcgcagt acgaggcgat
     1621 ctccgccttg ccggtgtcgg gatcgatctc gacctcggcg atgtgacagc cgttcgggaa
     1681 ggtggacccg aacttgcctt ccgccacgac gttcattgaa ccgttctttg caagatcacc
     1741 caggcggagc ttccgtttcg attctcgcgt atggaatacg cccctcgcgt attccacctg
     1801 cgagggctcg acgccgagct cgagggcggc gagcgcgcgg ccgtgctcga tcagcttgcg
     1861 cgcggcgaga tggcagacac tgcctgcgcc cactgtgctg cgcgagccgc cggtatggtt
     1921 accctggagt tcatgctgct tgccgggggc gcactggcga atgctgaccc gctcgagcgg
     1981 gatcccgagc gcgtcggcta cgatcatcgc gaaagtcgtc tcgtggccat gcccctgtgc
     2041 cttcgccatt gtgtaggcga cgacgttgcc tgccgcatcg accgtgagct ccacctcgtc
     2101 cttcgcggcc tggccggcgc cggtgttctc aatcacggtc gcgatgccga tgccgcgcag
     2161 cttcccaccc ttctccgcgc gcgcacgccg ctttggatag cccttccagt ccgcgagttt
     2221 gagcgccttc tcgaacacgc ccggcaggtc cgcgttttcg taggtcgatc cggtcggcgt
     2281 cttgtacggg aacgcctccg gggggatgaa attgcgccgc cgcagctcgg ccgcatccat
     2341 cttcagctcg gccgccgccc ggctcaccag gcgctccacg gtgtaggcga tgtcgggacg
     2401 gcccgcgccc cggtacgacg agaccgggtt ggtgttggtc agcgcgaccc gatagtgggc
     2461 gtagagcgca gggatgcggt agacgccggt catgcaggtc gaggtgttgc gaatgtggcc
     2521 catggaagcg ggcgacaggt aggcgcccat gtcgttcacc cagtcgagcc gcattgcgag
     2581 gaaacgcccg tcgccgtcga gcgcgagcgc tccgtcgatg atgttggccc ggccgtgggt
     2641 gtcgcacgca atgccctcgg agcgcgtcga gacccatttc accggcctgc cggtttcctt
     2701 cgccgcgagc atcagcgcgc agtgctccgg atacgccggc gtgcgctggc cgaatccgcc
     2761 gccgacgtcg cgcacctcga agttgagctt ctcctccggc acgccggtat aggccgacag
     2821 gtgccggcgc agcgtggtga tgccctggca gcacacgtgc agcgtgtagc tgtcgctgcg
     2881 cgcatcatag gcggcgacgt aggcgcgcgg ctccatcggg ttcggtgcga cccgggtcac
     2941 ctcgaccttc agccgcgtca cgtgcgcggc ccgcgcgaac gccgattcga cggcggcggc
     3001 atcacccgct tccgcttcca gcgccaggtt gcccggcacg tcctcgtgca actggggcgc
     3061 gcccggcgcc agcgcctcct cgggatggac tgcgggcggc agatcacggt actcgacttc
     3121 gaccagttcc gcggcgtcct gtgccgccgc tgcggtgtcc gccaccacca tcgccagtgc
     3181 ctcgccgacg aagcgcacct tgcggtgggc gagcgcgggc cgtgcgggag cgcgggtctt
     3241 catgccgccc ttccccggga aggtcagcgt gtgcat
//
LOCUS       DBNGWZ010000352         3181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1803801, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000352 DBNGWZ010000000
VERSION     DBNGWZ010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1803801"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1191
                     /locus_tag="AC16CX_10105"
     CDS             268..1191
                     /locus_tag="AC16CX_10105"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3012898.1"
                     /translation="MLMPIKLRGVMQAPVTPMKDDFSFDGDGFAKMVDFHVRHGAPAI
                     AWPHHKAESLNLTIAERKLGAEIAVKTVNKRVPVSIFVGTLSEEDSLDLACHAQKTGA
                     DAILTISPYCRRPSQEEIYDSVVRIGSSVELPVLTYNSPWRNGEGVEFTGELTRRLID
                     RLPNYIGMKDASFHGGKYLEISRVALKMRPGFAIIMGVEHLLASYPLGACGSFSSSGA
                     IAPNLCNRFFASLEAHDWDTARECQYKISGLWRLFKEQYPSSLKGAMIMMGRPVGPTR
                     SPLPTASKERLDFLRQQLEELDILQTEPHGW"
     gene            1374..2309
                     /locus_tag="AC16CX_10110"
     CDS             1374..2309
                     /locus_tag="AC16CX_10110"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3012899.1"
                     /translation="MTGFEPGLVHAPVTPFRRDQSVDYNTYAKLIEFHLRNGAEALAV
                     PMPEGEDMSLRNGEQREILEFAIKRVKGRVPVLAHVSDAGTQIAVDRAVHAEKAGAAA
                     IVSHPPYFWHPRPAMVVEHLARIGGAVRLPFYVCRPPVESAGTTLSTELVLQLIERVP
                     NLAGVVDASLDFVFMEEVISNGRRLRPGFQLLAGQDYLVPAAALGGNGAIVPLAGVAP
                     KLVRQVYDLCKQEKYVEARAMQENIAELHHLVKEAGESGLKDGLAGIKSAMAAMGRAC
                     GVPRPPVRPLGEVERGRFAEAIQAMAFLKAEPRGW"
     gene            2454..>3181
                     /locus_tag="AC16CX_10115"
     CDS             2454..>3181
                     /locus_tag="AC16CX_10115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3012900.1"
                     /translation="MPESSDRISQHYGSAGLADRILSALKQAGKNPSSVTVEDLAPLD
                     QFHTRGLAATRELITFAGVKPGWRVLDVGSGLGGPARVLASEKNCHVTGVDITKEFCE
                     VATLMSKWTALEQITEFRHGDATALPFENGQFDLVWTMQIQMSIENKRQFYDEIFRVL
                     KPGGRFVFQDIMGGPGGEVHLPVPWTNSRESSFLISIDALREMLKEVGFQIETLDDFS
                     EEALAWRKTQPAAAGLTLPTLGMHV"
BASE COUNT          619 a          941 c         1054 g          567 t
ORIGIN      
        1 tgggacagat tgtcaccggc gccgtaaccg tgccgaagga cgggatggtc gtattcaagt
       61 cggtcggcac cgcgttgcag gatcttgcgc tggccgcacg ctactacgag ctcatcggcg
      121 cgagcgcggg tgtgccgacc gcggcggacc tcgcaagcgc gaagtaacgc gcgttcacat
      181 tgtgacaagg cgttccggcg ccttgacccc gccagccggc tggagaagaa tgccagcgct
      241 cccgaacaac gttaaaggaa gctgaccatg ctcatgccga tcaagctcag gggcgtcatg
      301 caggccccgg tcacgccgat gaaggacgac ttcagcttcg acggggacgg attcgccaag
      361 atggtggact tccacgtgcg ccacggggcg cccgccatcg cctggccgca ccacaaggcg
      421 gaatcgctca acctcacgat cgcggagcgc aagctcggcg ccgagatcgc ggtcaagacg
      481 gtcaacaaac gggtgccggt ttccatcttc gtcggcacgc tgtcggagga ggactcgctc
      541 gacctcgcgt gtcatgccca gaagaccggc gctgacgcca ttctcacgat ctcgccgtac
      601 tgccggcgcc cgtcgcagga ggagatctat gattccgtcg tgcgcatcgg gagctccgtc
      661 gagctgcccg tgctcaccta caactcgccg tggcgcaacg gcgagggcgt ggaattcacc
      721 ggcgagctga cccggcgcct gatcgaccgg ctgcccaact acatcggcat gaaggacgcg
      781 agcttccacg gcggcaagta cctggagatc tcccgcgtcg cgctcaagat gcgccccggc
      841 tttgcgatca tcatgggcgt cgagcacctg ctcgcctcct atccgctggg ggcctgcggc
      901 tcgttctcgt cctccggcgc catcgcgccc aatctctgca acaggttctt cgcgtcgctg
      961 gaggcgcacg actgggatac ggcgcgcgaa tgtcagtaca agatttcagg gttgtggcgc
     1021 ctgttcaagg agcagtatcc ctcgtcgctc aagggtgcca tgatcatgat gggccggccg
     1081 gtcgggccca cgcgctcgcc gctgcccacg gcgagcaagg agcgcctgga tttcctgcgg
     1141 cagcagctcg aggagctgga catcctgcag accgagccgc acggctggta agagcaggga
     1201 tgaggggaga acacgtgagg gcgggagggc gtgagagcga tagaagccgt gagagcggga
     1261 gagcgtgagc gcgtgagatc gagttagtga gttacgattt ttccccctcc cgatctcccg
     1321 acttctcgat cttccgtttt ttggtaacca gtcatcatga ttaggcttat acgatgaccg
     1381 ggttcgagcc tggattggtg cacgcgccgg tcaccccgtt ccggcgcgac cagagcgttg
     1441 attacaacac ctacgcgaag ctgatcgaat ttcacctgcg caatggcgcc gaggccctcg
     1501 cggtgccgat gcccgaaggc gaggacatga gcctgcggaa tggagagcag cgggaaatcc
     1561 tcgagttcgc gatcaagcgg gtaaaggggc gcgttccggt gctcgcgcac gtgagcgacg
     1621 ccggcaccca gatcgcggtg gaccgcgccg ttcacgccga gaaagccggt gcggcggcga
     1681 tcgtgtccca cccgccgtat ttctggcatc ccagacccgc catggtggtg gagcacctgg
     1741 cgcggattgg aggggcggta cgcctgccgt tttacgtctg cagaccgccg gtcgagtcag
     1801 cgggaacgac gctttccacc gagcttgtgc tgcagttgat cgagcgcgtg ccgaacctgg
     1861 cgggagtggt ggatgccagc ctcgacttcg tgttcatgga ggaagtcatc tcgaatgggc
     1921 gccggctgcg cccgggcttc cagctgctgg ccggccagga ttatctcgtg ccggctgccg
     1981 cgctcggcgg caatggcgcg atcgtgccgc tggcgggcgt tgcaccgaag ctggttcggc
     2041 aggtttacga tttgtgcaag caggagaaat acgtcgaggc gcgcgcgatg caggagaaca
     2101 tcgccgagct gcatcatctg gtgaaggaag cgggcgagtc cgggctcaag gacggccttg
     2161 ccggcatcaa gtctgccatg gccgcgatgg gccgcgcgtg cggcgtgccg cggccgccag
     2221 tgcggccttt gggcgaggtc gagcgcggga ggttcgctga ggcgattcag gccatggcgt
     2281 tcctcaaggc cgagccgcgc gggtggtgag ggccgtgata agcaaccgca gataaacgca
     2341 gataaaagcg gataagaacg acagacgaac cgcagagacg ccgagagaac ctacaaataa
     2401 atcctacatt gggtaactgc gcttcttcta taccccggtg taggaatgcc tcaatgcctg
     2461 aatcgagcga ccggatatcg cagcactatg gcagcgcggg ccttgcggac cgaattcttt
     2521 ccgcgctgaa gcaggccggc aagaacccga gttccgttac tgtcgaggat ctggcgccgc
     2581 tcgatcagtt ccacacccgc gggctggccg ccacacgcga actcatcacc ttcgcgggcg
     2641 tgaaaccggg atggcgagtg ctcgacgtgg gaagcgggtt gggaggtccc gcgcgagttc
     2701 tggcatcaga gaagaattgc cacgtgacag gggtggacat caccaaggaa ttctgcgagg
     2761 tggccacact gatgtccaaa tggacggcgc tggagcaaat cactgagttc cggcacggcg
     2821 atgcgaccgc gttgcccttt gagaatggcc agttcgacct ggtctggacc atgcaaatcc
     2881 agatgagcat cgagaataaa cggcagttct atgacgagat ctttcgggta ctgaagccag
     2941 gggggcggtt cgtattccag gacataatgg gcggccccgg cggagaagtt catttaccag
     3001 tgccttggac aaacagtcgc gaatcgagct tccttatctc catcgacgcg ctgcgcgaaa
     3061 tgctaaagga agtgggcttt caaattgaaa cgttagacga tttctctgag gaggctttag
     3121 cgtggcgaaa aacccagcct gcggcagcgg gacttacgct gccgacattg ggtatgcatg
     3181 t
//
LOCUS       DBNGWZ010000353         4965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2939189, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000353 DBNGWZ010000000
VERSION     DBNGWZ010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2939189"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16CX_10120"
     CDS             <1..233
                     /locus_tag="AC16CX_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR3012901.1"
                     /translation="RALLSAVPVVDPGTKREVIRLQGDLPSPVNPPAGCHFHPRCPAA
                     FARCSQAYPDSARFTASHTTACHLYAAGAVKR"
     gene            complement(416..1762)
                     /locus_tag="AC16CX_10125"
     CDS             complement(416..1762)
                     /locus_tag="AC16CX_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR3012902.1"
                     /translation="MPLRRSLTIALVIAFAAGCAQMPQGEPDSLAQPPSAVIRVPAPQ
                     VAQAPTPPPAPPAPAAVPEPPPPPANVWERIRAGFRLSNMNGPLVQDWEQYYSSRPDY
                     VARMVERGSRFLYHVVEEVERRKMPMEIALLPMIESAYNPQAYSRSHASGMWQFIPST
                     GKLYGLRQNFWYDGRRDVLAATSAALDYLEKLHGQFGDWNLALAAYNWGEGAVSRAIA
                     KNQAKGLPTDYENLTMPVETRKYVPKLQAVKNIVANPEQYGITLADIPNRPYFATVAT
                     QRHIDVKLAASLAGISVEEFRFLNPAHNKPVIKADGSETIVLPMDKIAVFQRNLEGHD
                     RPLVSWQAYTMKRSDKPESIAARHGMTLAQLKEVNGIPGKKKIVAGQTLLVPLRGTAE
                     PHLPDLPAPKVTLARYKPRKARCVQVRNGVRKVVQCPAVKKRAPAKRTNVAVQARG"
     gene            complement(2034..2843)
                     /gene="gloB"
                     /locus_tag="AC16CX_10130"
     CDS             complement(2034..2843)
                     /gene="gloB"
                     /locus_tag="AC16CX_10130"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR3012903.1"
                     /translation="MSPESNHPLPITHHASWDVVPLRAYSDNYIWTIRDARCAAVVDP
                     GDARPVLDYLNREKLELVAILNTHHHADHVGGNTVLLGHWKVPVFGPHDNRIREVTHR
                     LKDGERSTLPHFGIEFEVFEIPGHTRTHIAFYGGGMLFCGDTLFAVGCGRLFEGTPGQ
                     MHDSLSRLMRLPDDTRVYCGHEYTLSNIRFAKAADPGNAALHELEAKAKKQRDQDLPT
                     LPSTIAQEKATNPFVRVREPAVIASANRYAGKTLNDPVSVLAAIREWKNRF"
     gene            2978..3742
                     /locus_tag="AC16CX_10135"
     CDS             2978..3742
                     /locus_tag="AC16CX_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3012904.1"
                     /translation="MTRNDTSRAAAEWFATPLGRYLLAREQPYFDRTVADIFGYNAFQ
                     LGLPGFDLLRASRIPFRARVDLSGPVGLRADFRDLPIASNSADLLVLPHVLEFSENPH
                     QVLREVARVLLPEAQVVIASFNPWSLWGFQRLFHRDGRYPWHGRFINLPRLKDWLTLL
                     GLEITGGQMGCYVPPCAAEKWLERFAFMDAAGDRWWPIAGGVYFLQAVKRVHGLRIIM
                     PRWTDRLASNKSFAPAPKKVARPEEALTARNGVSGE"
     gene            3842..4294
                     /gene="rnhA"
                     /locus_tag="AC16CX_10140"
     CDS             3842..4294
                     /gene="rnhA"
                     /locus_tag="AC16CX_10140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HIR3012905.1"
                     /translation="MSKVVEIYTDGACKGNPGVGGWGALLRYEGKTRELYGGEALTTN
                     NRMELTAVIRALEALKRGCRVRLHTDSQYVQLGIRSWIHDWKKRGWRTADKKPVKNED
                     LWRRLDELAAGHEIEWIWVRGHAGHPENERADELANLGVAQVGSKGVT"
     gene            4457..>4965
                     /gene="dnaQ"
                     /locus_tag="AC16CX_10145"
     CDS             4457..>4965
                     /gene="dnaQ"
                     /locus_tag="AC16CX_10145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR3012906.1"
                     /translation="MRQIVLDTETTGLEPELGHRIIEIAGVELVNRRFTGNDFHRYLN
                     PERESEAGALEVHGLTAEFLSDKPKFRDIAAEFLDYVSGAELVIHNSAFDVAFLDREL
                     DLADLKPLADYCPVVADTLRMAKDLHPGKRNSLDALCERYQIDNSARTLHGALLDARL
                     LAEVYLAMTR"
BASE COUNT          888 a         1531 c         1593 g          953 t
ORIGIN      
        1 cgcgtgcgtt gctctcggcg gtgccggtgg tggacccggg gacgaagcgg gaggtgatcc
       61 ggctgcaggg cgacctgccg tcgccggtga atccgccggc ggggtgtcac ttccaccccc
      121 gctgtccagc ggccttcgcg cgctgctcgc aggcctaccc ggacagcgcg cgcttcaccg
      181 cttcacatac gacggcgtgc catctgtacg ccgctggtgc ggtgaaacga taaaggcgtg
      241 aatggtgatt ggtgaatagt gaatggaccc catcccgggc cccgttgggg gaggtaaacc
      301 tcgatcctgc tggctcgtta aagcctcatg caagcagcgc tatgcgactg cctgatacag
      361 caccaatcac caatcactaa tcaccaatca cgccgttata gcgtgggccg gccgctcagc
      421 cgcgcgcctg gaccgcgacg ttcgtgcgct tcgccggcgc gcgcttcttg acggccgggc
      481 actgcacgac cttccggacg ccatttctca cctggacgca tctcgccttc ctgggcttgt
      541 agcgggcgag agtcactttc ggcgccggca ggtcgggaag gtggggctcg gccgtccccc
      601 tcagcggaac gagcagggtc tggccggcga cgatcttctt cttgccgggg atgccgttga
      661 cttccttcag ctgagcgagc gtcatgccgt gtctcgccgc gatgctctcc ggcttgtcgc
      721 tgcgcttcat ggtgtaggcc tgccatgaca ccagcggccg gtcgtgcccc tcgaggttgc
      781 gctggaacac agcgatcttg tccatgggga gcacgatggt ttccgagccg tcggctttga
      841 tcaccggctt gttgtgggcc ggattgagga agcgaaattc ctccaccgat atcccggcga
      901 ggctcgcggc gagcttgacg tcgatatggc gctgggtggc gacggtggcg aaatagggcc
      961 ggttcgggat gtcggcgagc gtgatgccat actgctccgg attggcgacg atgttcttaa
     1021 ccgcctggag cttgggcacg tatttgcgcg tttccaccgg catggtcagg ttctcgtaat
     1081 cggtgggcag ccccttcgcc tggttcttgg cgattgcccg gctcaccgcc ccctcgcccc
     1141 agttgtaggc ggcgagcgcg agattccagt cgccgaactg gccgtgcagt ttttcgaggt
     1201 aatcgagcgc ggcgctggtc gcggcgagca cgtcgcgccg gccgtcgtac cagaagttct
     1261 ggcgcagtcc gtagagcttg ccggtcgagg gaatgaactg ccacatgccg gaagcatggg
     1321 agcgcgaata agcctgcggg ttgtaggcgc tctcgatcat cggcaacagc gcgatctcca
     1381 tgggcatctt gcgccgctcg acttcctcga cgacgtggta gaggaagcgg ctgccgcgct
     1441 ccaccatgcg cgcgacgtag tccggccggc tggagtaata ctgctcccaa tcctgcacca
     1501 gcgggccgtt catgttggac aggcggaagc cggcacggat gcgctcccag acgttcgccg
     1561 gcggtggagg cggttcggga acggctgccg gggccggcgg cgcgggcggg ggcgtcggcg
     1621 cttgcgccac ctgcggtgcg ggaacgcgta tcaccgcgga tgggggctgg gcgagcgagt
     1681 caggctcgcc ctgcggcatc tgtgcacaac ccgccgcgaa agcgatcaca agagctattg
     1741 tgaggcttct tcttaacggc attcggttcc tcccgtcctc ggttccaggt atccaaaact
     1801 cccctcctta ccgaggaggg gtgcttacgc accgcccata cgctttttac atgacggcga
     1861 gatagtagcc aagagcacta gttccgtcaa gagaaatata cttaataatc atcatattct
     1921 aatggaaatc gaaagtggtt tcataaccct aaaatgcgtg aatggtgaat ggtgaatgga
     1981 tatggttttt caggtttact ccaatcacta atcaccaatc actattcacg ccgtcaaaac
     2041 ctgttcttcc actcccgtat cgccgccagc acgctcactg gatcgtttaa cgtctttccg
     2101 gcgtaacggt tggcagaagc gatcacggcg ggttcgcgca cccgcacgaa cgggttggtc
     2161 gctttctcct gagcgatggt ggagggcagg gtgggcaagt cctggtcgcg ctgcttcttc
     2221 gcttttgcct ccagttcgtg caacgccgcg ttgccggggt ccgcggcctt ggcgaagcgg
     2281 atgttggaca gggtgtattc gtgcccgcaa tacacccgcg tgtcgtccgg caggcgcatc
     2341 aggcgggaca gcgagtcgtg catctgcccg ggcgtgccct cgaacagccg cccgcagcct
     2401 acggcgaaca gggtgtcgcc gcagaacagc atgccgccgc cgtaaaacgc gatgtgggtg
     2461 cgcgtgtggc cggggatctc gaacacctcg aactcgatgc caaagtgcgg cagggtgctg
     2521 cgctccccgt ctttcagccg gtgcgtgacc tcgcggatcc ggttgtcgtg cggcccgaat
     2581 accggcacct tccagtgccc gagcaagacg gtgttcccgc cgacgtgatc ggcatgatgg
     2641 tgggtattga gaatcgcgac cagctcgagc ttttctcggt tgagatagtc gagcaccggc
     2701 cgtgcatcgc ccggatccac taccgcggcg cagcgcgcgt cgcggatcgt ccagatgtag
     2761 ttatcggagt aagcgcgaag ggggactacg tcccaggagg cgtgatgggt gatgggtaat
     2821 gggtgattgg actcagggct caagggcgtg attcgtgatt ggagatgagt gatttaagtc
     2881 aataaacccg aacaaggcat ccggcaagaa tccattcatc aatcactatt caccattcac
     2941 gcttttcaat gtcatgcggc gattataaac tcgcatcgtg accaggaacg atacctcccg
     3001 cgctgccgcg gaatggttcg ccacgccgct cgggcgctat cttctcgcgc gcgagcagcc
     3061 gtatttcgac aggacggttg ccgacatctt cggctacaac gcgttccagc tcgggctgcc
     3121 tgggttcgat cttctgcgcg cgagccgcat tccgttccgg gcgcgcgttg atctctcggg
     3181 cccggtcggg ctcagggccg atttccgaga cctgccgatc gcgagcaaca gcgccgatct
     3241 cctggtgctg ccgcacgtgc tcgagttcag cgagaacccg caccaggtcc tgcgcgaggt
     3301 ggcgcgggtg ctgctgccgg aagcccaggt ggtgatcgcc agcttcaatc cctggagcct
     3361 gtggggcttc cagcgcctgt tccaccgcga cgggcgctac ccgtggcacg gccgtttcat
     3421 caatctcccg cggctcaagg actggctgac gctgctcggg ctggaaatca cgggcgggca
     3481 gatgggctgc tacgttccgc cgtgcgccgc ggaaaaatgg ctggaacgct tcgcattcat
     3541 ggacgcggcg ggcgaccgct ggtggccgat cgcgggcggc gtctacttcc tgcaagcggt
     3601 gaagcgcgtg cacggcctgc ggatcatcat gccgaggtgg accgaccggc ttgcatcaaa
     3661 caagagcttc gctcccgcgc ccaagaaggt cgcgcggccg gaggaagcgc tgaccgcccg
     3721 caacggcgtg agtggtgagt agtgaatagt gagtggactg aacctgcttg gggccttcat
     3781 tgacgatagg caagggccgc ctttgtccaa tcactactca ccaatcacta atcacgcagt
     3841 catgagcaag gtggtggaaa tctatacaga cggcgcctgc aagggcaatc ccggcgtcgg
     3901 cggttggggc gcgttgctcc gttacgaagg caaaacgcgg gagctttacg gtggcgaggc
     3961 gctgaccacc aacaaccgga tggaactgac cgccgtcatc cgcgcgctcg aggcgctgaa
     4021 gcgtgggtgc cgcgtgcggc tgcacaccga ctcgcaatac gtgcaactgg gtatccgctc
     4081 gtggatccat gactggaaga agcgcggctg gcgcaccgcg gacaaaaagc cggtcaagaa
     4141 cgaggacctg tggcgccggc tcgacgagct cgcagccggg cacgagattg aatggatctg
     4201 ggtccgagga cacgccgggc acccggaaaa cgagcgtgcc gatgagctgg ccaatctggg
     4261 cgtagcccag gttggttcga aaggcgtgac atgatgaatc gtgacgtggt gattcgtgac
     4321 atgatcaacc gtgacatggt aaagcgtgag tttgttgacc acgccctgtc gcgtcacgct
     4381 tcatcacgtc acgccgcacc atgtcacgct gtaccacgtc acgcattacc atgtcatgcc
     4441 ctatcaagtt gagcaaatgc gacagatcgt attggatacc gaaaccaccg gcctcgagcc
     4501 ggaactcggc catcgcatca tcgagatcgc gggcgtggag ctcgtcaacc ggcgtttcac
     4561 cggtaacgac ttccaccgct atctcaatcc cgagcgcgag agcgaggcgg gcgcgctcga
     4621 ggtccacggc ctcaccgccg agttcctgag cgacaagccg aagttccgcg acatcgccgc
     4681 cgagttcctc gattacgtga gcggcgccga gctcgtcatc cacaactcgg ccttcgacgt
     4741 cgccttcctc gaccgggaac tcgacctcgc ggacctgaag ccgctcgccg actactgccc
     4801 ggtcgtggcg gacacgctgc gcatggcgaa ggatctccac cccggcaagc gcaacagcct
     4861 cgacgccctg tgcgagcgct atcagatcga caactccgcc cgcacgctgc acggcgcgct
     4921 gctggatgcg cggctcctcg cagaggtcta tcttgcgatg acgcg
//
LOCUS       DBNGWZ010000354         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_294260, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000354 DBNGWZ010000000
VERSION     DBNGWZ010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_294260"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC16CX_10150"
     CDS             <1..704
                     /locus_tag="AC16CX_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3012907.1"
                     /translation="HWSTFIASRVVTDYDEKLQDEIAALPGGEYIHRCWQCGSCTNSC
                     TVNAVNPDFNPRYWIYLIRMGMESELLRDKDIIWQCVSCHKCVYACPRDVFPEGVMKA
                     TAHWLELKGHTPKSKSMIFDEEFSEQVFATGKIEEGRVIRGFFRRTGQPLLQDWLVEM
                     ARRVARRLPVKLAMLLGLAALIRPRTRGWAGARAAIGEYVAEQEASQRKALGLDRLVE
                     MAKSELPGAPDAKRA"
     gene            728..1678
                     /locus_tag="AC16CX_10155"
     CDS             728..1678
                     /locus_tag="AC16CX_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR3012908.1"
                     /translation="MAGASTKGKKDQYRRVAYYPGCALEGSGHAYNRSTKEVGRALGL
                     ELDEVKNWNCCGAMEVKNIDPKIQTYLSSRVMSIAANEMGASVVMAPCNGCYHNLKKA
                     EYDLAQDGASREVVDRLSRKAGHATYQAGQVETIHALDWIKEGIGEGELKRRVRNSLK
                     GLKVANYYGCLYTRPRHIFPEKDRGPGSESTSQPHFMDDLLAAAGAENVDFPLKTACC
                     GGAHTLSDCDTSTKLVLNILRAAEAAGADVIATECPTCHSGLEMHQVRAEKVLGQKTS
                     VKMLYFTQLLGLALGLSPRKVGVHENVSGSLDLLKERGLA"
     gene            1675..2091
                     /locus_tag="AC16CX_10160"
     CDS             1675..2091
                     /locus_tag="AC16CX_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012909.1"
                     /translation="MRSLAAIEAELDSFAECADAREAARHLLAAGEEVLEHWVSARGE
                     TATTEQREGFRLLALHRQGAQGDPSFNACRETCREIAYHYNLLTLEADPEAAPRRQRM
                     MMMLVKHLALFVSGKMQVEKLGEFCCSSRPLRLESP"
     gene            2119..>2411
                     /locus_tag="AC16CX_10165"
     CDS             2119..>2411
                     /locus_tag="AC16CX_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033293 - monocarboxylic acid binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl attachment domain-containing
                     protein"
                     /protein_id="HIR3012910.1"
                     /translation="MATVRGCNLPDDLLYDVDNHIWFQELPDGNVKLGMTTVATAMAG
                     QLVAFTPKKVGRKVDPGKTCATVESGKWVGPAKSAAGGEVAAVNDELVNKPSLA"
BASE COUNT          448 a          789 c          821 g          353 t
ORIGIN      
        1 gccactggag cacgttcatc gcctcgcgcg tcgtcaccga ctacgacgag aagctgcagg
       61 acgagatcgc ggcgctgccg ggcggcgagt acatccaccg ctgctggcaa tgcggctcgt
      121 gcaccaactc ctgcaccgtc aacgcggtga atcccgattt caatccgcgc tactggatct
      181 atctcatccg catgggcatg gaatcggagc tgctgcgcga caaggacatc atctggcagt
      241 gcgtctcctg ccacaagtgc gtctacgcct gcccgcgcga cgtgtttccc gagggcgtga
      301 tgaaggccac cgcgcactgg ctggagctga aggggcacac gcccaagtcg aaatcgatga
      361 tcttcgatga ggagttctcc gagcaggtgt tcgcgaccgg gaagatcgag gaaggccgcg
      421 tcatccgcgg cttctttcgc cgcacgggac agccgctcct gcaggactgg ctggtcgaga
      481 tggcgcggcg ggtcgcgcgg cggctgcccg tgaagctggc gatgctgctg gggctggccg
      541 cgctgatacg cccgcgtacc cgcggctggg ccggcgcgcg cgcggcgatc ggcgagtacg
      601 tcgcggagca ggaggcgagc cagcgcaagg cactcggcct cgaccggctg gtcgagatgg
      661 cgaagagcga actccccggc gcgccggacg cgaagcgggc atagccgact gaagagagta
      721 accaggaatg gcgggagcca gcaccaaggg caagaaggac cagtaccggc gcgtcgcgta
      781 ctaccccggc tgcgccctcg aaggctcggg ccacgcctat aaccgctcga cgaaggaggt
      841 cggacgtgcg ctcggcctcg agctcgacga ggtgaagaac tggaactgct gcggggcgat
      901 ggaagtgaag aacatcgacc cgaagatcca gacttatctg tcgtcgcggg tgatgtcgat
      961 cgccgcgaac gagatgggcg caagcgtggt catggcgccg tgcaacggct gctaccacaa
     1021 tctcaagaag gccgagtacg acctcgcgca ggacggggct tcgcgcgagg tggtggaccg
     1081 cctgtcgcgg aaagccggcc acgccaccta tcaggccggc caggtcgaga ccatccacgc
     1141 gctcgactgg atcaaggaag gcatcggcga gggcgagctc aagcgccgcg tgcgcaactc
     1201 gctcaaggga ctcaaggtcg ccaactacta cggctgcctg tacacgcgcc cgcgacacat
     1261 ctttccggaa aaggaccggg gccccggcag cgagtccacc tcacagcccc atttcatgga
     1321 cgatctgctt gcggcggccg gtgccgagaa cgtggatttt ccgctgaaga ccgcctgctg
     1381 cgggggcgcc cacacgctct ccgactgcga cacctcgacc aagctggtgc tcaacatcct
     1441 gcgcgccgcc gaagccgccg gcgcggacgt gatcgcgacc gaatgcccga cctgccactc
     1501 ggggctcgag atgcaccagg tgcgcgcgga gaaggtgctg ggccagaaga cgagcgtgaa
     1561 gatgctctac ttcacccagc tgctcggcct tgcgctcggg ctttctccgc gcaaggtggg
     1621 cgtgcacgag aacgtgtcgg ggtcgctcga cctcctgaaa gaaagagggc tcgcgtgaga
     1681 tcgctcgctg ccatcgaagc ggagctcgat tcgttcgccg agtgcgcgga cgcgcgggag
     1741 gcggcgcggc acctcctcgc cgccggcgag gaggtgctcg agcactgggt cagcgcgcgc
     1801 ggcgagacgg cgaccacgga gcagcgcgaa gggttccgcc tgctggcgct gcaccggcaa
     1861 ggcgcgcagg gcgatccgag cttcaacgcc tgccgcgaga cctgccgcga gatcgcctat
     1921 cactacaatc tgctcaccct cgaggccgat ccggaggctg ccccgcggcg ccagcgcatg
     1981 atgatgatgc tggtcaagca cctcgcgctc ttcgtcagcg gcaagatgca ggttgagaag
     2041 ctcggcgagt tctgttgctc gtcgcgaccg ctccgcctag aatcgccgtg accatcgcaa
     2101 cagcagagga ctcggaacat ggccaccgtg cgcggctgca acctcccgga cgacctgctc
     2161 tatgacgtcg acaaccacat ctggttccag gagcttcccg acggcaatgt caagctcggc
     2221 atgaccacgg tggcgaccgc catggccggg cagctcgtcg cgttcacgcc caagaaagtc
     2281 ggccgcaagg tggatcccgg caagacctgc gcgacggtcg aatccggcaa gtgggtgggc
     2341 ccggccaagt cggcggccgg cggcgaggtg gcggcggtga acgatgagct cgtcaacaag
     2401 ccttccctcg c
//
LOCUS       DBNGWZ010000355         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2309399, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000355 DBNGWZ010000000
VERSION     DBNGWZ010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2309399"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..361)
                     /locus_tag="AC16CX_10170"
     CDS             complement(59..361)
                     /locus_tag="AC16CX_10170"
                     /inference="COORDINATES: protein motif:HMM:NF014092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR3012911.1"
                     /translation="MESRVIFIGDEVAAAGYRLAGLSVRAPGPGEETAALREELARAL
                     LILLSQRMTSRIARSELRDALARTSPLVLVVPDGAGLPPDIDIAARVRLQLGVGEA"
     gene            complement(366..716)
                     /locus_tag="AC16CX_10175"
     CDS             complement(366..716)
                     /locus_tag="AC16CX_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR3012912.1"
                     /translation="MNPRVRFAIGLTLAAALFSAAGTFLLAGPLAAAQPAVPGLSLTE
                     EAVGWGLFSAALATGLSSLGAGIAVGRLGTAAIGALAEKPELFGRLLIFVGLAEGIAI
                     YGLIVSILILNRLA"
     gene            complement(700..2508)
                     /locus_tag="AC16CX_10180"
     CDS             complement(700..2508)
                     /locus_tag="AC16CX_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR3012913.1"
                     /translation="MIRPHPAEWFEILVAKDDSVLLLEALAWNGCVELEGEAREPAAD
                     LQRSLARFDAMAQRFAPFWPEAETIARPAEIHEMAEHGVAVIEAWSAAATGSIEAIRA
                     LEAEQSNLALWRQVLLELRDNALDFSALVAPGKGVECALLMFPEDAAPALPPAVLGRR
                     LKLGADAAMLAVGPPEALAVLTQQVMSAQGKRIETPAWLRASAAESLAYASARIVAID
                     DKLVAARAELGKIATRYRLGEALGWLRHAQWMMRSIGVVDSGEHFCRVTGWTDDRGRL
                     SRALDASGARALVHFPVPPGGAQAPLLLQNPWWARPFEVFSRALGMPARYAADPSVLL
                     AVIVPLLFGYMFGDVGQGAVLIAAGLLLRRRAPVLGMLVAGGISAMLFGLMFGAVFGL
                     HGVVPALWLMPLEDPLPVLAVPLAGGAVLLLLGLAINALEAYWRGKLRHWLVSDAGLI
                     VVYVGILAGFLHTAGYVVAAIGTALPVIGYIALTRRLRPAIAAIGELLERTLQLLINT
                     ISFVRVGAFALAHAGLSAAIIALAEAASSAVGHGTVMVLGNVVVIAVEAMVVSIQTTR
                     LVLFEFFTRFFVSKGREFHPLPPPIFVRGELHESTS"
BASE COUNT          460 a          937 c          823 g          353 t
ORIGIN      
        1 tttgccttcc gcttgtcctc gacgatctgg agcaaggccg cggcggcctc tgcgggattc
       61 atgcctcccc cacgcccagt tgcagccgca cgcgcgctgc gatgtcgatg tccggcggca
      121 agcccgcccc atccggcacg acgaggacga gcgggctcgt tctcgcgagc gcgtcccgca
      181 gttccgatcg cgcgatccgg ctggtcatcc gctgcgagag caggatcaac agggcgcgcg
      241 cgagttcctc gcgtagcgcg gctgtctctt cgccgggacc gggtgcccgc acggaaagcc
      301 ccgccagccg gtagccggcc gcggcgacct cgtccccgat gaagataacg cggctttcca
      361 tccgctcacg cgagacggtt gagaatcagg atcgacacga tcagcccgta gatcgcgatc
      421 ccctccgcta ggcccacgaa gatcagcagc cgcccgaaca gctccggctt ctctgcgagg
      481 gccccgatcg cggcggtccc cagcctgccg actgcgatgc cggcccccaa cgaggacagg
      541 ccggtggcga gcgccgcact gaacagtccc catcccaccg cctcctccgt cagcgacagc
      601 ccgggtacgg cgggctgcgc ggcggccaat ggcccagcta ggaggaaggt acccgcggcg
      661 ctgaagagcg cggcggcgag tgttaaaccg atagcgaacc taactcgtgg attcatgcaa
      721 ctctcctcga acgaaaatcg gcggcggcag gggatgaaat tcgcgaccct tgctgacgaa
      781 aaaacgcgtg aaaaactcga acaggacgag ccgcgtggtc tggatcgaga ccaccatggc
      841 ctcgacggcg atcaccacga cgtttcccag aaccatcacg gtgccgtgcc cgacggcgct
      901 ggaggcggcc tcggcgagcg cgatgatcgc ggccgacagg cccgcgtgcg ccagcgcgaa
      961 ggccccgacg cgcacgaagg aaatcgtgtt gatgaggagc tgcagcgtcc gctccagcag
     1021 ttccccgatg gcggcgatcg ccggccgcag ccgtcgcgtg agcgcgatgt atccgatcac
     1081 cgggagagcc gtaccgattg ccgctaccac gtaaccggcc gtgtgcagga aaccggcgag
     1141 aatgccgacg tagaccacga tcaggccggc atcggacacc agccaatgcc ggagcttgcc
     1201 gcgccaatat gcttcgagcg cattgatggc gagccccagc agcaacagca ctgccccgcc
     1261 ggcgagcggc acggcgagca ccggcagtgg gtcctccagc ggcatcaacc acagcgcggg
     1321 cacgacgccg tgcaggccga agacggctcc gaacatcaac ccgaacagca tggccgagat
     1381 tccccccgca accagcattc ccagcaccgg cgcgcgcctg cgcagcagca ggccggcggc
     1441 gatcaggacc gccccctgtc cgacgtcgcc gaacatgtag ccgaacagaa gcggcacgat
     1501 gactgcgagc agcacgctcg ggtccgccgc gtagcgtgcc ggcatgccga gcgcgcggct
     1561 gaacacctcg aagggccgcg cccaccaggg attctgcagc aggagcggcg cctgcgcgcc
     1621 accgggcggc acgggaaaat ggacgagcgc acgcgcgccg gaagcatcga gtgcccggct
     1681 gagccgtccg cgatcgtcag tccacccagt gacgcggcag aaatgctcgc cgctgtccac
     1741 cacgccgatg ctgcgcatca tccattgcgc gtggcggagc caccccagcg cctcgcccag
     1801 gcggtagcgg gtggcaattt tgccgagttc cgcccgtgcg gcgaccagtt tgtcgtctat
     1861 cgcgacgatg cgggcggagg cgtatgcgag gctttcggcc gcgctcgcgc gcaaccaggc
     1921 cggcgtctcg atccgtttcc cctgcgcgct catcacctgc tgcgtgagca cggcgagggc
     1981 ttccggcggt ccgaccgcca gcatggcagc atccgccccg agctttagcc tccgcccgag
     2041 cacggcggga ggcagggccg gcgccgcgtc ctccggaaac atgagcagag cgcactccac
     2101 ccccttgccg ggcgcgacca gcgcagagaa atcgagggcg ttgtccctga gttccagcag
     2161 cacctgccgc cacagcgcca gatttgactg ctcggcctca agcgcccgta tcgcctcgat
     2221 cgatcccgtc gcggcagctg accatgcctc gatgacggcc acaccgtgtt ccgccatctc
     2281 gtggatttcg gccggacgcg cgatcgtctc cgcctccggc cagaacgggg cgaaacgctg
     2341 ggccatggca tcgaaacggg caagcgagcg ctgcaaatcc gccgcgggct cgcgcgcttc
     2401 gccctcgagc tcgacgcatc cgttccacgc aagcgcctcg agcaggagca cgctgtcgtc
     2461 cttcgcaacg aggatctcga accactccgc gggatgcggc cgtatcatgc cgctgccccc
     2521 tctgctccac cgctcatgcc gctgcctcca aacggatatc tgagttgcgt cca
//
LOCUS       DBNGWZ010000356         2700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_43243, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000356 DBNGWZ010000000
VERSION     DBNGWZ010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_43243"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..406
                     /locus_tag="AC16CX_10185"
     CDS             185..406
                     /locus_tag="AC16CX_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012914.1"
                     /translation="MCVGSFCYGPLVSPISASGLGEQADPPEVWRLAVIDGADFASLL
                     DRLGGIQTMSTSPRATERHGRISIALPMS"
     gene            complement(551..1795)
                     /locus_tag="AC16CX_10190"
     CDS             complement(551..1795)
                     /locus_tag="AC16CX_10190"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3012915.1"
                     /translation="MADRFYPRHLEPRLRAALRDTPVVMLSGARQSGKSTLALRIAAE
                     AGLPYVTLDDATTLAATRDAAAFLRGLGERAVIDEVQRAPWLFPAIKMAVDHARRPGR
                     FLLTGSANVLLVPKVSESLAGRMEILRLWPLSQGEILGIRERFLEALFARHRPALPPN
                     RTREQDIESVITRGGYPELVARDSAARREAWLSAYVTTLIERDVRNLANIEGLSDLPR
                     VLGLLAARTGALLNVAEVSRDSGVAHTTLKRYLALLKAAYIIAPLPAWSRNVGKRLIK
                     TAKLYFPDSAVAAYLGRASTEGLRADRGMLGRLLENFALGELRKQSDWAERAVEIHYF
                     RTAGGQEVDFVLEHRDGRIAGIEVKAARTLSGSEFGGLQMLAAATGGKFVRGVVLYLG
                     ERALQFADRLWAMPVSALWRMQ"
     gene            complement(1918..2328)
                     /locus_tag="AC16CX_10195"
     CDS             complement(1918..2328)
                     /locus_tag="AC16CX_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3012916.1"
                     /translation="MDRLLADTGFLIAAGQHGDPLHENAKSFLQDYSGRLVTVSAVIV
                     ETCFFFGSDGKLKLLDWVRSGGLAVAEIPVPAYPALAAIIEKYADHNIDFTDAALVWL
                     AGESGLRRILTVDRADFSAFRIKGGKRLELIDWY"
     gene            complement(2321..2572)
                     /locus_tag="AC16CX_10200"
     CDS             complement(2321..2572)
                     /locus_tag="AC16CX_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012917.1"
                     /translation="MPISVRLPLRVEQELSDYCVSHKVTKSEAVKRALEQMLDRSSGK
                     ASAFELGKRFIGVEKSRGDVARHSQRLLREHFRNKGSLG"
BASE COUNT          483 a          894 c          828 g          495 t
ORIGIN      
        1 ggtcgggcct gctctggcaa cgccacaaag aacccgtcgt cccggatatt ccttgctcgc
       61 ggccgcgcac gtcctgcctg tgcgtccgtc gccgtctact gttcaccatt caccattcac
      121 cattcaccag ccgtttacca ttcaccggtt ccatccgcgt ccatctgcgt ggatctgcgg
      181 tttcatgtgt gttgggtctt tctgctacgg ccctttggtt tccccgatct cagcgagcgg
      241 gctcggcgag caggcggatc cacccgaggt gtggcgcctt gcggtaatcg acggcgccga
      301 cttcgcctca ctgctcgatc ggctgggggg aattcagacg atgtccacct cgccgcgggc
      361 gaccgagcgc cacggcagga tctcgatcgc cttgccgatg tcgtaggcct tccgccgcgc
      421 cgcgagggat ccatttcata gcttggatta ctgcagtgac agttcataaa tacaaggcta
      481 ctttggaccc ggcccgggcc cgcctaccga aggccctccc gacggcgcgg tgaagtgggc
      541 ttcgtctgca ctattgcatg cgccacagcg cgctcaccgg catcgcccac aggcggtcgg
      601 cgaactgcag cgcgcgctcg cccaggtaaa gcaccactcc gcggacgaac ttgccaccag
      661 tcgccgcggc gagcatctgc agcccgccga attcgctgcc cgataaagtc cgcgccgcct
      721 tcacttcgat cccggcaatg cgtccatcgc gatgttccag cacgaaatcc acctcctggc
      781 cgcccgctgt tcgaaagtag tggatttcca cggcgcgttc cgcccaatcg gattgcttgc
      841 gaagctcgcc gagcgcgaag ttttcgagca gacgcccgag catcccgcga tcggcccgca
      901 gaccctcggt gcttgcgcgc ccgagatacg ccgcgactgc gctgtcggga aagtaaagct
      961 tcgcggtctt gatcagccgc ttgcccacgt ttcgcgacca ggcgggcagc ggcgcaataa
     1021 tgtaggccgc cttgagcaat gcgaggtagc gcttgagcgt cgtatgcgca acgccactgt
     1081 cgcgcgatac ctctgcgaca ttgagcaggg cgccggtgcg cgccgcgagc aagccgagca
     1141 cgcgcggcag atcgctcagc ccctcgatgt tggcgagatt gcgtacatcg cgctcgatca
     1201 gcgtggtcac ataggccgag agccacgctt cgcgccgcgc ggcggagtcg cgcgccacca
     1261 gctcgggata gcctccgcgc gtgatgacgg actcgatatc ctgttcgcgc gtccggttgg
     1321 gcggtagcgc cggccggtgt ctcgcgaaca gcgcttcgag aaaacgctcc cgaatgccca
     1381 ggatctcacc ctgcgagagc ggccacaggc gcaggatttc catgcggccg gcgagcgact
     1441 cggagacctt cggcacgagc agaacgttgg ccgatccggt gagaaggaac cgtccaggcc
     1501 ggcgcgcatg gtccaccgcc attttgatgg ccggaaagag ccacggggcg cgttgcacct
     1561 cgtcgatcac ggcgcgctcg ccgagtcccc gcaggaacgc cgccgcgtcg cgcgtggcgg
     1621 cgagcgtggt ggcgtcatcc agcgtgacgt agggaaggcc cgcctccgcg gcgatcctga
     1681 gagcgagggt gctcttgccg ctctggcgcg caccgctcaa catcaccacc ggggtgtcgc
     1741 gcaaggccgc gcgcagtctc ggctcgaggt ggcgcgggta aaaacgatct gccattgagt
     1801 ggcagattat ccactatata ttgggtatgc gtcaagtggg caccggccac gccctggcta
     1861 cgattcacga tttacgattc acggattctt gaaccgccaa gacaattgcg actggcgcta
     1921 ataccaatcg atcagctcca agcgcttccc gcccttgatg cggaatgccg aaaagtcggc
     1981 gcggtcaact gtcaggatcc tgcgcaagcc gctctcgccc gcgagccaga ccagcgcggc
     2041 gtccgtgaaa tcgatgttgt gatcggcgta tttttcgatg atcgcggcaa gcgccggata
     2101 cgccggaacc gggatttcag ccactgcgag gccgccggaa cgcacccaat ccagcagctt
     2161 tagctttccg tcgctcccga aaaagaagca tgtttcgacg atcacggcgg acaccgtgac
     2221 gagcctgccc gaatagtcct gcaggaatga tttcgcattc tcgtgcagcg ggtcgccgtg
     2281 ctgtcccgcc gcgatcagga agccggtgtc ggccagcagc ctatccaagc gagcccttgt
     2341 tgcggaagtg ctcgcgaagc aaccgctggg aatggcgcgc cacgtcgccg cggctcttct
     2401 cgacgccgat gaatctcttg cccagctcga aggcgctggc ttttccggaa gagcgatcga
     2461 gcatctgctc gagggcgcgc ttcacggcct cgctcttggt aaccttgtgc gagacgcagt
     2521 agtccgagag ctcttgctcg acacgcaagg ggaggcggac ggaaatgggc atggccgcgg
     2581 cctgtatgac aaaaccggag gtattgtact acactccctc cgttccgcgc aaatgagtaa
     2641 tgcgttattg ttggcgcatc actcatcacc catcaccacg gcaacgccgg caggactgag
//
LOCUS       DBNGWZ010000357         3244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2685859, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000357 DBNGWZ010000000
VERSION     DBNGWZ010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2685859"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC16CX_10205"
     CDS             <1..214
                     /locus_tag="AC16CX_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012918.1"
                     /translation="RREDIAVETERYGRHGYAVSGANVRSSRLHRMLADAGEKRPVAY
                     TTLGADERWVKDLILATWARESVSGE"
     gene            complement(313..924)
                     /locus_tag="AC16CX_10210"
     CDS             complement(313..924)
                     /locus_tag="AC16CX_10210"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3012919.1"
                     /translation="MPPVTKTLIIVIVAIFFVQQLADDALIIWFALWPLATTSPGAPG
                     VPAGFLPWQLVTYSFLHGNGLHLLVNMLGLYMFGSEVERVLGRRRYFAYYFVSVLSAA
                     VTQLVISALSAGPAYPTIGASGGVFGLLLAYGIFFPRRIVMLIFPPIPMPARMFVIVY
                     AAIELYLGVTGTQAGVAHFAHLGGMLGGFLLLRYWRRQRLPPG"
     gene            955..1275
                     /locus_tag="AC16CX_10215"
     CDS             955..1275
                     /locus_tag="AC16CX_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012920.1"
                     /translation="MAAGTECNSKKRRGRPGLYRKPERSIRGQLFCELQSTTPFFDFN
                     SLRKQQGYPYRTALQETRALLLQKTSQHPRRFLVPAARAQALADTSKGRRALNIRKAD
                     VSLH"
     gene            complement(1318..1788)
                     /locus_tag="AC16CX_10220"
     CDS             complement(1318..1788)
                     /locus_tag="AC16CX_10220"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HIR3012921.1"
                     /translation="MAIDIGIKPQDRKAIAQGLSRLLADTYTLYLKTHNFHWNVKGPM
                     FQTLHLMFETQYNELALAVDQIAERIRALGHPAPGSYADYARLSSIKDAKGVPSATRM
                     ITELVEGQEAVVRTAREIFPLVEKANDEPTADLLTQRMQTHEKTAWMLRSLLEK"
     gene            1901..2875
                     /locus_tag="AC16CX_10225"
     CDS             1901..2875
                     /locus_tag="AC16CX_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012922.1"
                     /translation="MPLRLLATAVLAAATCGAFAQAPATGAEGYPSRPVRFIVPLPPG
                     GSPDTIARTIANEINWPFPVVVDNRTAGGRVLAADLVAKSAPDGHTWFLTTDNIMVVI
                     PQLDKTPYHPLKDFTPVTQTARFQFLLVVHPSVPARSVKELVALAKSKPGSLNYGSSG
                     NGSAQHLGTALLQYLAGIEMTHVPYKGAGPALVDLLPGRIHVFIGAANQLLPQIKEGR
                     LRLLASAGAQRLPLLPDVPTIAESGVPGYHHDVWSGVMMPAKVPPAIVAKANADITRV
                     LNAPDVKARLASQGIDVKTGSPQELARLIREDYARWGKVIKATGIRAE"
     gene            complement(2961..>3244)
                     /locus_tag="AC16CX_10230"
     CDS             complement(2961..>3244)
                     /locus_tag="AC16CX_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR3012923.1"
                     /translation="YKYIHYVGMPPMLFDLEADPHERRELACESAHKKIIAECEGLLR
                     KIVDPEAVDKLARADQKAHIEKNGGEEAILKKGTFRYSPPPGAEAAYYY"
BASE COUNT          640 a         1013 c         1010 g          581 t
ORIGIN      
        1 gcggcgcgag gacatcgcgg tggaaacgga gcgctacggg cgccatggct acgccgtgtc
       61 gggtgccaac gtgcgttcgt cgaggcttca tcgcatgctt gccgatgccg gggaaaagcg
      121 gccggtggca tacaccaccc tcggcgccga cgagcggtgg gtgaaggacc tcatcctcgc
      181 cacctgggcg cgtgaatccg tgagtggtga atagtgagta gtgagtggac agaaccgttg
      241 ataacgccgc cttcaccgga taaaggcgta acctgaaaga atccactcac cattcaccaa
      301 tcactacacg ctttatccag gcggtagccg ctggcggcgc cagtagcgca gcagcagaaa
      361 gccgccgagc atgccgccga gatgggcgaa gtgggcgacg cccgcctgcg tgccggtgac
      421 gccgaggtag agctcgatcg ctgcgtaaac gatcacgaac atccgcgccg gcattggaat
      481 cgggggaaag atcagcatga cgatgcggcg cgggaagaag attccgtaag cgagcagcag
      541 gccgaacacg ccgcccgagg cgccgatcgt gggataggcc ggacccgcgc tcagcgcgga
      601 tatcacaagc tgggtaacag ccgcggaaag caccgacacg aagtagtatg cgaaatagcg
      661 gcggcggccc agcacccgct cgacctcgga accgaacatg tacaacccga gcatgttcac
      721 caggagatgc aacccgtttc cgtgcaggaa gctgtaggtc acgagttgcc agggcagaaa
      781 gccggcgggc acacctggag cgccggggga agtcgttgcc aatggccaaa gggcgaacca
      841 gatgatcagg gcatcgtcgg cgagctgctg cacgaaaaag atcgcaacga tgacgatgat
      901 cagggtcttg gtgaccgggg gcatctggcg tgtggaaggg cgcgcggcgg gctgatggcc
      961 gctggcaccg agtgtaacag caaaaaacgg cgtggtcgcc ccggtctgta tcgcaagccc
     1021 gaaaggtcaa ttcgcggtca acttttctgc gagcttcaga gcaccacgcc gttttttgat
     1081 ttcaattcac taagaaaaca gcagggttat ccgtaccgta ccgcgctgca agaaacgcgc
     1141 gccttacttc tccagaagac ttctcagcat ccacgccgtt ttttagttcc ggccgcgcgc
     1201 gcccaggcgc ttgccgacac aagcaaagga aggcgcgcgc ttaacatccg caaagcggat
     1261 gttagccttc actgaaagac ggcgttcaac tggcatcatg ccagcaaagt ccgtgcgtca
     1321 tttttctaat agacttctga gcatccaggc cgtcttttca tgcgtctgca tccgctgggt
     1381 gagcaggtcg gcagtcggct cgtcgttcgc tttctcgacg agcgggaaga tctcacgcgc
     1441 ggtgcgcacc accgcttcct gcccctcgac cagttcggtg atcatcctcg tcgcgctggg
     1501 cacgcccttc gcgtccttga tcgaggagag cctggcgtaa tcggcgtagc tcccgggcgc
     1561 ggggtggccg agcgcccgga tgcgctccgc aatctgatcc accgcaagcg cgagctcgtt
     1621 atactgcgtc tcgaacatga ggtgcagcgt ctggaacatg ggtcccttca cgttccaatg
     1681 gaaattgtgg gtcttcaggt agagcgtgta agtgtccgcg agcaggcgcg acagcccctg
     1741 cgcgatcgcc ttgcggtcct gcggcttgat cccgatgtcg atggccatga tggtctcctg
     1801 tatgggcgtt ggcagggaca atatagggga cggccagtaa cagagcaatc aaattaaact
     1861 acacgcactg atcgtaaaaa ccgatcatag gaggaagcgc atgccactac gactgctcgc
     1921 taccgccgtg ctcgccgctg cgacgtgcgg ggccttcgca caggctcccg cgacgggtgc
     1981 ggagggctac ccgtcgcgtc ctgtgaggtt catcgtgccg ctgccgccgg gcgggtcgcc
     2041 cgacaccatc gcgcgcacga tcgcgaatga gatcaactgg ccgttcccgg tcgtggtgga
     2101 taaccgcacc gccggcggcc gcgtgctcgc tgcagacctg gtcgcaaaga gcgcgcccga
     2161 cggtcacacg tggttcctca cgaccgacaa catcatggtc gtgatccccc agctcgacaa
     2221 gacgccgtac cacccgctca aggacttcac gcccgtgacg cagaccgcgc gcttccagtt
     2281 cctgctcgtg gtccacccct cggttccggc aagatcagtg aaggaactgg tggcgctcgc
     2341 caaatcaaag ccggggtcgc tcaactacgg atcgtccggc aacggcagcg cgcagcacct
     2401 cggcaccgcg ttgctgcaat acctcgctgg catcgagatg acccacgtgc cgtacaaggg
     2461 cgcgggcccc gcgctggtgg atcttctgcc cggacgcatc cacgttttca ttggagcggc
     2521 caaccaactg cttccgcaga tcaaggaggg gcggctccgg ttgctcgcct ccgccggcgc
     2581 gcagcggctg ccgctgctgc cggacgtgcc cacgatcgcc gagtcgggcg tgcccggcta
     2641 ccaccacgac gtctggtctg gtgtgatgat gccggcgaaa gttccgcccg cgattgtcgc
     2701 caaggcaaac gcggacatca ccagggtgct caacgcaccc gacgtcaagg cgcggcttgc
     2761 ttcgcaaggc atcgatgtga agaccggctc gccgcaagag ctggcgcggc tcatccgcga
     2821 ggactacgcg cgctggggca aggtcatcaa ggcgacggga atccgggccg aatagtccga
     2881 aggcgaaagg cgaaaggcga aaggggtaag aacactgcct gacggttctt acttccgcct
     2941 ttcgacttcc gactttcgcc ttagtagtag tacgcggctt ccgcccccgg cggcggcgaa
     3001 tagcggaacg tgcctttctt caggatcgct tcctccccgc cgttcttctc aatgtgcgcc
     3061 ttctggtcgg cccgcgcgag cttgtccacc gcttcgggat ccacaatttt tctcaggagc
     3121 ccttcgcatt ctgcaatgat tttcttgtga gcggactcgc atgcgagttc tctgcgctca
     3181 tgcgggtccg cttccaggtc gaacagcatc ggcggcatcc ccacgtagtg gatgtacttg
     3241 tacc
//
LOCUS       DBNGWZ010000358         3701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2310064, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000358 DBNGWZ010000000
VERSION     DBNGWZ010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2310064"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="AC16CX_10235"
     CDS             complement(<1..96)
                     /locus_tag="AC16CX_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR3012924.1"
                     /translation="MRYLQKGFTLIELMIVVAIIGILAAVAIPAYQ"
     gene            431..748
                     /locus_tag="AC16CX_10240"
     CDS             431..748
                     /locus_tag="AC16CX_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012925.1"
                     /translation="MGLFGSVSSKQVDEFAKSLAHKLAKQYPPEHERDGVQKLSPKRF
                     TAILDETFNKAAQFKKQHKLGVYKKARLGNTFRWELQDLGYSDNFVKTITEALIIHIA
                     KKD"
     gene            complement(761..2410)
                     /locus_tag="AC16CX_10245"
     CDS             complement(761..2410)
                     /locus_tag="AC16CX_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3012926.1"
                     /translation="MNDLSRSRGGDDLLKRLERLYQIGIALSKEKDITRLLEAILIAA
                     QEITRADGGTLYRMTEEKTLRFEIMRNDTLGMAMGGTSGVEIPFYPVHLYDKAGGPIL
                     SNVSAYAVHHDKSVNIADAYTEEGFDFTGTKNFDKKTGYRCRSFLTVPMKNHEGEIIG
                     VLQLINSKDPATGEVVAFSDTDQHLAESLASQAAIALTNRLLINHLENLFESFIGLIN
                     AAIDDKSPYTGGHCQRVPVLTMMLAEAVDHCKVGPLKDFIMSERDRYELKIAGLLHDC
                     GKVTTPVHVVDKATKLQTIFDRVALIDTRFEVVKRDVEIALLRERLKALDKGAAGVLG
                     AEIESRLKPIEDERDFLRRANIGGEAMKDEDITRVREIAAKYRWRDPEGDRADFLSED
                     EIKNLTIRYGTLTPEERQIINHHIEVTIQMLEALPWPKHLRNVPEYAGGHHERMDGKG
                     YPRGLTREQMSVQARCMGIADIFEALTAKDRPYKKGKTLTESLTILGRFKLNGHIDPD
                     LFDVFIWEKVYEKYARQFLDPDQIDEVDVSKIPGYRSPPSN"
     gene            complement(2419..>3701)
                     /locus_tag="AC16CX_10250"
     CDS             complement(2419..>3701)
                     /locus_tag="AC16CX_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR3012927.1"
                     /translation="EDVASAANDNELVKLVNKIIIDAYQQGASDIHIEPYPGKAKTEI
                     RFRKDGELMQYISVPGSYRSALAARLKIMCDLDISERRKPQDGKIKFKKFGPLDIELR
                     VATIPTQGGVEDIVMRILAAGEPLPLDKMGFSKRNLMLCKREVDKPYGLFFVCGPTGS
                     GKTTTLHSVLGYLNTPDMKIWTAEDPVEITQKGLRQVQMNPKAGLTFAIAMKAFLRAD
                     PDVIMVGEMRDKETVSTGIEASLTGHLVFATLHTNSAPESIIRLLDMGMDPFNFADAL
                     LGILAQRLARRLCKCKEAHVATPEEIQALMEEYSLELQSTATWKKDPKAAREKLYEEW
                     VKEYGNEKGELILHTAKGCDVCGKTGYKGRLGLHELLIGTDRMKKNIQEHARVAEMFA
                     TALEDGMRTLKQDGIEKVLQGITDIQQVRAVCIK"
BASE COUNT          635 a         1152 c         1111 g          803 t
ORIGIN      
        1 ctggtaggcc gggatcgcca ccgccgccag aataccgatg atcgcgacca cgatcatcag
       61 ttcgatcagc gtaaagccct tttggagata acgcattgag tgcccctttc gtggttgacc
      121 attcaaacgc gagcaatttt cattgctcgc ggcactatag cacgcaattt ccgtgcccag
      181 ccttgcatct cttcggaacg cggaatatta ccctgtacaa aatgggatgg ccggcttatt
      241 gcgcgccgtt tgcccctttt tgagtccctg tctcctgcag gcccgcgtcg gccaagggca
      301 ctatgtgaca aaattcgtca cttgcgggat ttcgcgaagc ggcagtccac tcggcgggat
      361 tcacgccgcg tccagtaaaa tccatgctgg cctggtttaa cacaatttgc cggcctacag
      421 gagcatatcc atgggattgt tcggttctgt ttcgagcaaa caagtggacg aattcgccaa
      481 gagcctggcc cacaagctgg caaagcagta tccgccggaa cacgaacggg acggcgtaca
      541 gaagctctcg cccaagcgct tcaccgcaat actcgacgaa acatttaaca aggcggctca
      601 attcaaaaag cagcacaagc tcggcgttta caagaaggcg cggctcggca acacgttccg
      661 ctgggaactg caggacctgg ggtacagcga caacttcgtg aagacgatca ccgaggcact
      721 catcatccat atcgcgaaaa aagactgaat gtccccggcg tcagttggac gggggcgatc
      781 ggtagccggg aatcttgctg acgtccactt catcgatctg gtcgggatcg aggaattgcc
      841 tggcgtactt ctcgtacacc ttttcccata tgaagacgtc gaagagatcc gggtcgatgt
      901 ggccattcag cttgaacctg ccgagtatgg tgagcgactc tgtgagcgtc ttgcccttct
      961 tgtaggggcg gtctttcgcg gtcagcgcct cgaaaatgtc ggcgatgccc atgcagcgcg
     1021 cctggaccga catctgctcg cgcgtgagcc cgcgcggata gcccttgccg tccatgcgct
     1081 cgtgatgtcc accggcgtac tcgggcacgt tcctcaggtg cttgggccac ggcagcgctt
     1141 ccagcatctg gatcgtaacc tcgatgtggt ggttgatgat ctgccgctct tccggcgtca
     1201 gcgtgccgta gcggatcgtc aggttcttga tctcgtcctc gctcaggaag tccgcccggt
     1261 cgccttccgg atcgcgccaa cggtacttcg ctgcgatctc ccgcacccgc gtgatgtctt
     1321 cgtccttcat cgcttcgccg ccgatattgg cccgccgcag gaagtcgcgc tcgtcctcga
     1381 tcggcttcag ccgcgactcg atctcggcgc cgagcacacc ggcggccccc ttgtcgagcg
     1441 ccttcaggcg ctcccggagc agcgcgatct cgacgtcccg cttcaccacc tcgaagcgcg
     1501 tatcgatgag ggcgacccgg tcgaagatgg tctggagctt ggtcgccttg tccaccacgt
     1561 gcaccggtgt ggtgaccttg ccgcaatcgt gcagcaggcc cgcgatcttg agctcgtagc
     1621 ggtcgcgctc gctcatgata aagtccttga gcgggccgac cttgcagtgg tcgaccgcct
     1681 cggcgagcat catggtgagc accggcacgc gctggcagtg accaccggtg tagggcgact
     1741 tgtcgtcgat cgccgcgttg atgagaccga tgaaggactc gaacaggttt tcgaggtggt
     1801 tgatgagcaa ccggttggtc agcgcgatcg ccgcctgcga cgccagcgac tcggccagat
     1861 gctggtcggt gtcggagaac gcgacgacct cgccggtcgc cgggtccttg gagttgatga
     1921 gctgcagcac gccgatgatt tcgccctcgt ggttcttcat cgggaccgtg aggaaggacc
     1981 tgcagcggta cccggttttc ttgtcgaagt tcttggtgcc ggtgaaatcg aatccttctt
     2041 cggtgtaggc gtcggcgatg ttgaccgatt tgtcatggtg cacggcgtac gccgacacgt
     2101 tcgaaaggat aggcccgccc gccttgtcat aaagatgaac cgggtaaaac gggatctcca
     2161 cgccgctcgt gccgcccatc gccatgccga gcgtgtcgtt gcgcatgatc tcgaacctga
     2221 gcgtcttctc ctcggtcatg cggtagagcg tgccgccgtc ggcgcgcgtg atctcctgcg
     2281 ccgcgatcag gatcgcctcg agcagccggg tgatgtcctt ctctttcgag agggcgattc
     2341 cgatctgata gagccgctcg agacgcttga gcaggtcgtc cccgccgcgg gaccggctca
     2401 ggtcattcat gacatgcgct acttgatgca caccgcacgc acctgctgga tgtccgtgat
     2461 tccctgcagc accttttcga tgccgtcctg cttcagggtt ctcatgccgt cttcgagggc
     2521 cgtggcgaac atttccgcca cgcgcgcgtg ctcctggatg ttcttcttca tgcggtcggt
     2581 gccgatcagc agctcgtgca ggccgagccg gcccttgtag ccagtcttgc cgcagacgtc
     2641 gcaccccttc gcggtgtgga gtatcaactc gcctttctcg ttgccgtatt ctttcaccca
     2701 ttcctcgtag agcttctcgc gcgcagcctt ggggtccttc ttccaggtcg ccgtactctg
     2761 cagctcgagg gagtactcct ccatcagcgc ctggatctcc tccggggtcg cgacgtgcgc
     2821 ttccttgcac ttgcacagcc gccgcgcaag acgctgggcg agtatgccga gcagcgcgtc
     2881 ggcgaagttg aacgggtcca tgcccatgtc gagcaggcgg atgatggatt cgggcgcgct
     2941 gttggtatgc agcgtggcga acaccaggtg gccggtgagc gaggcctcga tgcccgtcga
     3001 gaccgtctcc ttgtcccgca tttcccccac catgatcacg tccggatcgg cccgcaggaa
     3061 ggccttcatg gcgatcgcga aggtgagccc ggccttgggg ttcatctgca cctggcgcag
     3121 ccctttctgc gtgatttcga ccgggtcctc ggcggtccag atcttcatat ccggcgtgtt
     3181 gaggtagccg agcaccgagt gcagcgtggt ggtcttgccc gagccggtcg gcccgcacac
     3241 gaagaaaaga ccatagggct tgtcgacctc ccgcttgcac agcatcagat tgcgcttgga
     3301 aaagcccatc ttgtcgagcg gaaggggctc gcccgcggcg agtatccgca tcacgatgtc
     3361 ttccacgccg ccctgcgtcg gaatggtcgc gacccgcagt tcgatatcga gcggtccgaa
     3421 tttcttgaac ttgatcttgc cgtcctgcgg cttgcggcgc tcggagatat cgagatcgca
     3481 catgatcttc aaccgcgccg cgagcgcgct gcggtagctg cccggcaccg agatgtactg
     3541 catgagctcg ccgtccttgc ggaagcggat ctcggtcttg gccttgccgg ggtagggctc
     3601 gatgtggatg tccgaggccc cctgctgata ggcatcgatg atgatcttgt tgacgagctt
     3661 caccagctca ttgtcgttcg ccgccgaagc gacgtcttcc g
//
LOCUS       DBNGWZ010000359         3107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2686354, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000359 DBNGWZ010000000
VERSION     DBNGWZ010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2686354"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="AC16CX_10255"
     CDS             <1..862
                     /locus_tag="AC16CX_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012928.1"
                     /translation="RRDRLPAAVLAARLPRIMTARASSLRVPRQPRTPVVRRAMPGAG
                     RVHAIERPQSGRLGGPRHDVCGRLIDRYGGRYSTELGLRLAANRHREIFQWFLAAVLF
                     GAPIRATAAAATYRALQGAGIDSPRAVIATGWNGLVTLLDEGGYARYDYKTATKLLEV
                     CGAIQERYRGSLAALHAAASDSADLEARLLALGTGIGPVTANIFLREMRGIWPKAQPL
                     PLPIVIHAAHHLGAVPVRVSAPAAVLDALQRLWDRHPAAGRDFADFEAALVRAGLDLQ
                     HRLGDFSKPE"
     gene            876..1433
                     /locus_tag="AC16CX_10260"
     CDS             876..1433
                     /locus_tag="AC16CX_10260"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HIR3012929.1"
                     /translation="MTGPVNFREGFRPVRRDKLIQERVHDTYKTWGKLPEPTVCPDCG
                     AVYHKGRWQWISQPTAAHGQLCPACHRIRDRFPAGYVTLAGDFLTRHRDEILHLVRNH
                     GEKAQTEHPLERIIAVQEHNGGVLVTTTDIHLARSIGEALENAYQGTLEFHYNEGENL
                     LRVHWERNNSPRATRVAGARRILKS"
     gene            complement(1627..1908)
                     /locus_tag="AC16CX_10265"
     CDS             complement(1627..1908)
                     /locus_tag="AC16CX_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012930.1"
                     /translation="MKQVIPQTPRDYDAKRVIERPDGFYWQDAETGEFFGPFPTLLDA
                     VNDMHYSAEAEPEPGETLREAEDEIGVADWIDPETGEPAEEQVPRIEDR"
     gene            complement(1941..2783)
                     /locus_tag="AC16CX_10270"
     CDS             complement(1941..2783)
                     /locus_tag="AC16CX_10270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR3012931.1"
                     /translation="MRDGECTAFLQWALPRLEREWAGYRKVRRLVCKRVAQRLKSLGL
                     ADLDAYRRRLVADAEEWRELDALCSIPVSRFYRDRRVFDTLESTVLPVLAEAAAPRGA
                     IDAWSAGCASGEEPNTLALIWAVRLGPRFPRIGLRIVATDCDPGLIARARAGRYAPSS
                     LKELPEDLRAAGLEELDGMWRIRDRYRGVDFQQQDLRERMPEGPFDLILCRNVVLTYY
                     APAVQQALMARIVERLRPGGALVIGAHESPPPTIRNVAAWPGARMIYRKFDADQSGRS
                     RRCA"
     gene            complement(2803..>3107)
                     /locus_tag="AC16CX_10275"
     CDS             complement(2803..>3107)
                     /locus_tag="AC16CX_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005430990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR3012932.1"
                     /translation="QDAGIRIVLRALEEPLRQIVANAGEEPSVVLNRVAQGEGDFGFN
                     ALTNEYGDMVKMGVLDPCKVTHTALQNAASIAGLILTTDCMIARTPDNEHAPAQGF"
BASE COUNT          506 a         1091 c          987 g          523 t
ORIGIN      
        1 ccgccgtgat cgactacctg cagcggtatt ggccgctcga ttaccgcgca tcatgaccgc
       61 gcgcgcctcc agtttgcgcg tcccgcgcca gcctcgcacg ccggtggtcc ggcgcgccat
      121 gccgggcgca ggacgggtgc atgcaatcga acgcccgcaa tcaggccgcc tcggcggccc
      181 gcggcacgat gtttgcggcc ggctcatcga ccgctacggc ggacggtatt ccaccgagct
      241 cgggctgcgt ctcgccgcga accgccatcg ggagattttc caatggtttc tcgccgccgt
      301 cctgttcggc gcccccatcc gggcgacggc cgcggccgcg acctatcgcg cgttgcaggg
      361 ggccggaatc gacagcccgc gggcggtcat cgccaccggc tggaacggcc tcgtcaccct
      421 cctcgatgag ggcggatacg cgcgctatga ttacaagacg gcgaccaagc tgctggaagt
      481 ctgcggcgcg atccaggaac gctaccgtgg aagcctcgcc gcgctgcatg cggcggcttc
      541 ggacagcgcc gacctggagg cgagattgct ggcgctcgga acgggcatcg gccccgtcac
      601 cgccaacatc tttctgcggg agatgcgcgg catctggccg aaggcgcagc cgctgccctt
      661 gcccatcgtg atccacgcgg cacatcatct cggcgccgtg ccggtgcgcg tttcggcgcc
      721 agccgcggta ctcgatgcgc ttcagcggct ctgggatagg catcccgcgg cgggccgcga
      781 cttcgccgac ttcgaggctg cgttggtgcg ggcgggactc gatctgcagc accgcctggg
      841 cgatttttcg aaacccgagt gaggagacag accccatgac tggacccgtc aacttccgcg
      901 aaggcttccg ccccgtgcgc cgcgacaagc tcatccagga gcgcgtccac gacacctaca
      961 agacctgggg caagctgcct gagccgactg tgtgccccga ttgcggggcg gtctatcaca
     1021 agggccgctg gcagtggata tcgcagccga ccgcggcgca cgggcagtta tgcccggcct
     1081 gccaccgcat ccgcgaccgc tttccggccg gctacgtcac gcttgccggg gattttctca
     1141 cgcggcaccg cgacgaaatc ctccatctgg tccgcaatca cggcgaaaaa gcccagaccg
     1201 agcatccgct cgagcgcatc atcgcggtgc aggaacacaa cggcggggtc ctcgtgacga
     1261 ccaccgacat ccatctcgcg cgcagcatcg gggaagcctt ggaaaacgcc taccagggga
     1321 cgctcgagtt ccattacaac gaaggggaaa acctgctgcg cgtgcactgg gaacgcaaca
     1381 acagcccgcg tgctacacgc gtggcgggtg ctcgacggat cttaaagtct tgagcggcag
     1441 ccgagcgcgg ggcacgcggg ctgtaatttt ttgaacagaa ctccgcagca gacgagaatt
     1501 cccgcttatc cctgctgttt ctgtgcgttc ccttagtttt ttttctcagc ccgcatgtcg
     1561 ctggcgcctg ctcgcgcgcc tggatagcca gacctcaaaa ccgcgtcatt cgtgacacat
     1621 gcgggcttag cggtcctcga tacgcggcac ctgctcttcc gccggctcgc ccgtttccgg
     1681 gtcgatccag tcggccacgc cgatctcgtc ctcggcctcg cggagcgttt cgcccggttc
     1741 cggctccgct tcggcgctgt agtgcatgtc gttcaccgcg tcgaggaggg tcgggaacgg
     1801 cccgaagaac tcgccggtct cggcgtcctg ccaatagaat ccgtccggcc gctcgatcac
     1861 gcgcttcgcg tcgtagtccc gcggcgtctg aggtatcacc tgcttcacga tcctgcctgc
     1921 caagctcaca ttagcagcat ttaagcacac cggcgcgagc ggccactttg atccgcgtca
     1981 aacttgcgat agatcatccg cgcacccggc cacgcagcaa cgttccgtat ggtgggcggc
     2041 ggggattcgt gggcgccgat gacaagcgcc ccgcccggcc gcaggcgctc gacgatccgc
     2101 gccatcagag cctgctggac ggcgggcgcg taataggtca ggacgacatt gcgacagagg
     2161 atgagatcga acggcccttc aggcatgcgc tcccgcagat cttgctgctg gaaatcgacg
     2221 ccccgatacc ggtcgcgaat gcgccacatc ccgtccagct cctcgaggcc cgccgcgcgc
     2281 agatcctcgg gaagctcctt gaggctgctt ggcgcgtacc ggcctgcgcg ggcgcgcgcg
     2341 atgagccctg ggtcgcaatc cgtcgcgacg atacgcagcc cgatccgcgg gaaccgcggc
     2401 ccgagccgga ccgcccagat cagcgcaagc gtgttgggct cctcccccga cgcgcaaccc
     2461 gcgctccagg catcgatcgc cccgcgtggt gccgcggctt cggcgagcac gggcagaacc
     2521 gtgctttcaa gggtgtcgaa gactctgcga tcccggtaaa accgggaaac cggtatgctg
     2581 cacaacgcat cgagttctcg ccactcctcc gcgtcggcca cgaggcggcg ccggtaggca
     2641 tcgagatccg cgagcccgag cgatttcagc cgttgcgcga cccgcttgca gaccaggcgc
     2701 cggaccttgc gatacccggc ccactcgcgc tccaggcgcg gcaacgccca ttggagaaag
     2761 gcggtgcact caccatcccg cattcaaggg atcacatccc cgtcaaaatc cctgtgccgg
     2821 cgcgtgctcg ttgtccggtg tccgcgcgat catgcagtcc gtggtgagta tgagcccggc
     2881 gatcgaggcg gcgttctgca gcgccgtgtg cgtgaccttg cacgggtcga gcacgcccat
     2941 cttgaccatg tcgccgtact cgttggtgag cgcgttgaaa ccgaagtcgc cctcgccttg
     3001 cgcgacgcgg ttgagcacga cggacggctc ctcgcccgcg ttggcgacga tctggcgcag
     3061 cggctcctcg agcgcgcgca gcacgatgcg gatgccggcg tcctggt
//
LOCUS       DBNGWZ010000360         1741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2940274, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000360 DBNGWZ010000000
VERSION     DBNGWZ010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2940274"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16CX_10280"
     CDS             complement(<1..362)
                     /locus_tag="AC16CX_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012933.1"
                     /translation="MRARQWIVMALLGLACAAPASAGEKTEDPFAQVESLLNPGTLLG
                     GVVREEDVSLLFAHFRAALLAAHEGRETPPPPEALNRRAEAIAAELKTRGTLAGLLLL
                     TAFEAAAKQALREALAEPA"
     gene            complement(421..>1741)
                     /gene="thiC"
                     /locus_tag="AC16CX_10285"
     CDS             complement(421..>1741)
                     /gene="thiC"
                     /locus_tag="AC16CX_10285"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR3012934.1"
                     /translation="KTITPEFVRDEVARGRAIIPANINHPETEPMVIGRNFLVKINAN
                     IGNSAISSGIQEEVEKMTWAIRWGGDTVMDLSTGKHIHETREWIVRNSPVPIGTVPIY
                     QALEKVDGKAEELTWEIYRDTLIEQCEQGVDYFTVHAGVRLPFIPMTAKRMTGIVSRG
                     GSILAKWCLAHHQESFLYTRFEEICEILKAYDVSFSLGDGLRPGSIYDANDEAQIAEL
                     KTLGELTDIAWKHDVQVMIEGPGHVPMQLIKENMDLQLQYCREAPFYTLGPLTTDIAP
                     GYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDKEDVKDGIMAYKIAAHAADLAKG
                     HPGAQIRDNALSKARFEFRWEDQFNLGLDPDKAKAFHDETLPQEGAKLAHFCSMCGPH
                     FCSMKITQDVREYAQKQGLSEREALEKGMQEKAEEFKQQGGEIYTRV"
BASE COUNT          257 a          586 c          558 g          340 t
ORIGIN      
        1 gcgggctcgg ccagagcttc gcgcaacgcc tgtttagccg ccgcctcgaa cgcggtgagg
       61 agcagcagtc cagccagcgt cccgcgtgtc ttcagctcgg cggcgatcgc ctccgcgcga
      121 cggttgagtg cctccggcgg cgggggtgtc tcccggccct cgtgggccgc caacagcgcc
      181 gccctgaagt gggcgaaaag gagcgatacg tcttcctcgc ggaccacgcc cccaagcagg
      241 gtcccggggt tgagcagcga ttcgacctgg gcgaagggat cctccgtctt ttcgccggcg
      301 ctggccggcg cggcgcaagc aagcccgagt agcgccatca cgatccactg tctggcacgc
      361 atagaatacc ctcctctgcc tgggcgaacg gtactctaac gcccctcacc gccccccggt
      421 ttacacgcgg gtgtagatct cgcccccctg ctgcttgaac tcctctgcct tctcctgcat
      481 gcccttctcg agggcttccc gctcggacag gccctgcttc tgcgcgtact cgcgcacgtc
      541 ctgggtgatc ttcatcgagc agaagtgcgg cccgcacatc gagcagaagt gtgcgagctt
      601 cgcgccttcc tgcggcaggg tctcgtcgtg gaaggccttc gccttgtccg ggtcgagacc
      661 gaggttgaac tggtcctccc agcggaactc gaagcgcgcc ttggagagcg cgttgtcgcg
      721 gatctgcgcg cccgggtgcc ccttggcgag gtccgcggcg tgcgccgcga tcttgtaggc
      781 catgatgccg tccttcacgt cctccttgtc gggaagaccg aggtgctcct tgggcgtgac
      841 gtagcacagc atggcggtgc cgaaccagcc gatcatcgcc gccccgatcg cgctggtgat
      901 atggtcgtag ccgggcgcga tgtcggtggt gagcggcccc agcgtgtaga acggcgcctc
      961 gcggcagtac tggagctgca gatccatgtt ctccttgatg agctgcatcg gcacgtgccc
     1021 cggtccttcg atcatcacct gcacatcgtg cttccaggcg atgtcggtga gctccccgag
     1081 cgtcttcagc tccgcgatct gggcctcgtc gttggcgtcg tagatcgaac cgggacgcag
     1141 cccgtcgccg agggagaacg agacatcgta agccttgagg atctcgcaga tctcctcgaa
     1201 ccgggtgtag aggaagctct cctggtggtg cgcgaggcac catttcgcca ggatcgagcc
     1261 gccgcgcgac acgatcccgg tcatgcgctt cgccgtcatc gggatgaacg gcagccgcac
     1321 gccggcgtgc accgtgaaat agtccacgcc ctgctcgcac tgctcgatca gcgtatcgcg
     1381 gtagatttcc cacgtgagct cctccgcctt cccgtccacc ttttccagcg cctggtagat
     1441 cggcacggtg ccgatcggca ccggggaatt gcggacgatc cactcgcgtg tctcgtggat
     1501 gtgcttgccg gtggagaggt ccatcaccgt gtcgccgccc cagcggatcg cccacgtcat
     1561 cttctcgact tcctcctgga tgccggagga aatcgccgag ttgccgatgt tggcgttgat
     1621 cttcaccagg aagttgcggc cgatcaccat cggctcggtc tcgggatgat tgatgttggc
     1681 ggggatgatg gcgcgcccgc gcgcgacctc gtcgcgcacg aactcggggg tgatcgtttt
     1741 c
//
LOCUS       DBNGWZ010000361         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3444159, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000361 DBNGWZ010000000
VERSION     DBNGWZ010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3444159"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="argA"
                     /locus_tag="AC16CX_10290"
     CDS             complement(<1..898)
                     /gene="argA"
                     /locus_tag="AC16CX_10290"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003641.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR3012935.1"
                     /translation="MRRHRLSAQDRFIDWFQSVAPYIHAFRGKTFVIAFGGDVVADGR
                     FLDLTHDLNLLAALGVRLVLVHGARPQIEAKLKALRRRSRFHKGLRVTDDVALNCAKE
                     ASGRLRVEIEAALSMGLPGSPMAGADIRVASGNFITAKPIGVLDGVDFRHTGEVRKVD
                     AGAIQKRLEDNELVLLSPLGYSPTGEVFNLALEDVAASTAIALKAEKLIFLMDAVGVT
                     GRRGELLRDLTVRQAQALPAGMGPADSDAQIYLPYAVRACEHGVRRAHLISGHIDDGL
                     LLELFTHKGVGTMVTPDPIETLR"
     gene            1203..>2407
                     /locus_tag="AC16CX_10295"
     CDS             1203..>2407
                     /locus_tag="AC16CX_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012936.1"
                     /translation="MNARGLFVFISLFLLVPSGFAQTRDASTEKLGKVSFPTSCDPKV
                     QAQFERGVAMLHSFWFSAAEKTFREVVEQDPSCAIARWGFASILMLNPLAGTGAAPKR
                     VPLAQEAIEQARQIGAKTQRERDYVEAVAAYYEGWANRPERARQLTRAKAYEALAVRY
                     PDDDEAQIFYALYLAGTQTQADQTYAAYLKAASILEKQFAKYPDHPGVAHYLIHSYDA
                     PPIAQQGLPAARRYASIAPSAPHALHMPSHIFTRVGAWADSAATNKRSAEAAIKGNER
                     DEATHASDYRVYAYLQLARDSEARAVMEEIGQLSGFNPARNTAPYALAAMPARYAIER
                     GNWREAAQLQPRASNFLFPEALTYFARALGAARSGDPAAAEKDVQQLASLLDALKAAK
                     NTYWATEVEV"
BASE COUNT          438 a          796 c          770 g          403 t
ORIGIN      
        1 gccgcagcgt ctcgatcggg tcgggcgtga ccatggtgcc gacgccctta tgggtgaaga
       61 gctcgagcag cagcccgtcg tcgatgtgcc ccgagatgag gtgcgcgcgt ctcaccccgt
      121 gctcgcaagc gcgcaccgcg tacggtagat agatttgcgc atcgctgtcc gcaggaccca
      181 tgccggcagg cagcgcctgc gcctggcgta ccgtcaggtc ccgcagcagc tcgccgcgcc
      241 ggcctgtcac ccccaccgca tccatgagga agatgagctt ctcggccttg agcgcgatcg
      301 cggtggacgc agcgacgtcc tccagcgcca ggttgaacac ttcgccggtg ggcgaatagc
      361 cgagcggcga gagcagcacc agctcgttgt cctcgagccg cttctggatc gcgccggcgt
      421 cgaccttgcg cacttcgccg gtgtgcctga aatccacgcc gtcgagcact ccgatcggct
      481 tggccgtgat gaagttgccg ctcgccacgc ggatgtcggc gcccgccatc ggcgaaccgg
      541 gcaaccccat cgagagtgcc gcctcgatct cgacgcgcag ccgcccgctc gcctccttgg
      601 cgcagttgag cgccacgtcg tcggtgaccc gcaagccctt gtggaaccgg ctgcggcggc
      661 gcagcgcttt cagcttcgcc tcgatctgag ggcgcgcccc gtgcaccagc acgagccgca
      721 cgccgagggc cgcgagcaga ttcaggtcat gggtgaggtc gaggaaccgg ccgtcggcca
      781 cgacgtcgcc gccgaaggcg atgacgaagg tcttgccgcg gaaggcgtgg atatagggcg
      841 cgaccgactg gaaccagtcg atgaaccggt cctgcgcgga cagccggtgc cgccgcaagg
      901 ccctctgaac gggctcagcg atggtttcag ctgctccgct ttcggtttca gtcttgcgcg
      961 taatgaccgc cccctccccg tatcgtcagc gcggccggcc ttaaaaacca ccttagcgca
     1021 tttcgtcggg atttcaagca gatattaagc gagattgaag cagagggaaa gctggcgaga
     1081 cggcgcgatt gacaccccgc agagcgccgc gcagactttt tcacgcattc cgtgcgacat
     1141 tagctgcccg tggcgtgtgg gagcgggccc ggatcaataa gaattcttca agggagggga
     1201 ccatgaacgc tcgcggcctg ttcgttttca tttccctgtt tctgcttgta ccgtcaggct
     1261 tcgcacagac gcgcgatgcc tccacggaga aactcggcaa ggtgagtttc ccgacctcct
     1321 gcgatcccaa ggtacaggcc cagttcgagc gcggcgtggc gatgctgcac tcgttctggt
     1381 tcagcgcggc cgagaagacc tttcgcgagg tggtggaaca ggacccctcc tgcgcaatcg
     1441 caagatgggg cttcgcgtcg atcctgatgc tcaacccgct tgcgggaacc ggtgccgcgc
     1501 ccaagcgcgt tccgctggcg caggaggcga tcgaacaagc ccggcaaatt ggcgcgaaga
     1561 cccagcgcga gcgcgactat gtcgaagcgg tcgccgctta ctatgagggc tgggccaatc
     1621 ggcccgagcg cgcacggcag ttaacgcggg ccaaggcgta cgaggcgctt gccgtccgtt
     1681 atcccgacga tgacgaggcg caaatcttct acgcactcta tctcgccggc acgcaaacgc
     1741 aggctgatca gacctacgca gcgtatctaa aggcggcctc gatcctcgaa aaacaattcg
     1801 ccaagtatcc cgatcacccg ggtgtggcgc actacctgat ccacagttac gatgcgccgc
     1861 ccatcgcgca gcaggggctg cccgccgccc ggcgctacgc cagcatcgcc ccctcggcac
     1921 cgcacgctct gcacatgccc tcccatattt tcactcgcgt tggtgcgtgg gcggattccg
     1981 ccgcgaccaa taagcgctcc gcggaggcgg cgataaaagg taatgagcgc gatgaggcga
     2041 ctcatgcgag cgattacagg gtttacgcct atttgcagct cgcgcgggac agcgaggcgc
     2101 gagctgtcat ggaggaaatc ggacaactga gcgggttcaa tccagcacgc aacaccgcac
     2161 cctatgcgct cgcggcaatg ccggcgcgct atgcgatcga gcgcgggaac tggcgcgagg
     2221 cggcgcaact gcagccgcgc gcaagcaatt ttctcttccc agaggccctg acctatttcg
     2281 cgcgcgcgct cggggccgcc cgcagcggcg accccgccgc ggcggaaaaa gatgtgcagc
     2341 agcttgcgtc gctgctcgat gcgctaaaag cggcgaaaaa tacgtactgg gcgaccgagg
     2401 tggaggt
//
LOCUS       DBNGWZ010000362         3845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_44381, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000362 DBNGWZ010000000
VERSION     DBNGWZ010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_44381"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /gene="rmuC"
                     /locus_tag="AC16CX_10300"
     CDS             <1..554
                     /gene="rmuC"
                     /locus_tag="AC16CX_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3012937.1"
                     /translation="ENYHRMLAPGANDMERAAAQRQFRSDIRKHVDDIARKYILPDVT
                     SDGAVMFVPAEAVFAEIHAYHAEVVDDANAKRVWIVSPTTLMAVLNTARAVLKDVETR
                     KQIHVIREALKRLAVEFNRFDERMKDLARHIRQAADDADRIQVTGGKISQQFQRIESA
                     ELEPSDTAQLKVVELRDDGKGAK"
     gene            653..1456
                     /locus_tag="AC16CX_10305"
     CDS             653..1456
                     /locus_tag="AC16CX_10305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR3012938.1"
                     /translation="MQWFRNWRRERILKRSTLDERLWRVVLGRYPFTRALSAEERARL
                     RDWVILFLHDKSIVGAGGLALRDDMRMCIAAQACMLILNLDLDFYRGWLEVIVYPGEF
                     VAEYEYMDEDGVAHHVEEPMSGESWLAGPVILSWADTELRGDEHGYNVVIHEFAHKLD
                     MLNGDANGFPPLHAGMDRGEWSRAFSAAYEHFRGRVERGGPTGIDPYAAENPSEFFAV
                     LSEAFFEIPDIVLADYGEVYRQLAAFYRQDPAARRESRAAKTAGQVAAL"
     gene            1453..>3845
                     /locus_tag="AC16CX_10310"
     CDS             1453..>3845
                     /locus_tag="AC16CX_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3012939.1"
                     /translation="MTPSLADTKSRTLLLISFGLLLSVLTALIALGLARIESFNQQIN
                     ALTEVQGRRIGVISEVFLANGQRSALIDRLFAAESRQARQAMLTQYQAAVAAYTRAVG
                     KLRVLEVAAAAHAARDEAIAAAGTALALGDRIVALLMRGEIATASELNLTQAVMVDSR
                     LQETLYLLLEANHAQTAQSIAAANQGMRNGFLLIGAGGVLALIAGIVIAVLVIRIVST
                     TEARLEHEKELAEVTLHSIMDGVITTDAKGRIEYLNPVAEHYLGWSSAQAAGRRLPEV
                     FRIVDERTGSAIETLPLTAGHGAGETEPIAVRLIDRSGRECPVRYSHAPIRGRDGTVH
                     GMIVVFHDVSQIRAMAQQLIWQASHDALTGLVNRREFERRLFELIETARMQRREHALL
                     FMDLDNFKAVNDTCGHSAGDELLRQLTAIMLSRMRGSDTLARLGGDEFGALLEACPLE
                     QALRIANAMRETVREFRFVWEDKTFNVGVSIGLVPINAASGDVNQVLAAADACCYEAK
                     NRGRDRVQVHRPHDPDYSSRHEELQLVSQITHAFEHGRFRLYRQRIAPLDRAPRREPH
                     YEVLVRMLDRSGNPVPATGFMPAAERYNLLTSIERWVVSSLVEFLSRQWAAGAIPHDL
                     HGGEERGFYSVNISGASINDKSFPDFLRNLLTRYQLPSGLLCFEITETTAISNLSKAA
                     ELMHELKGMGCRFALDDFGTGMSSFAYLKYLPVDFLKIAGIFIKDMVTDPMDYAIVDS
                     INRISHILGMRTVAESVEDAATLTRITELGLDYAQGYFIAEPELLGDEPAQRQTALFA
                     "
BASE COUNT          680 a         1241 c         1300 g          624 t
ORIGIN      
        1 tcgagaacta ccataggatg ctcgcgccgg gcgcgaacga catggagcgc gcggccgcgc
       61 aacgccagtt ccgctccgac atcaggaaac acgtggacga catcgccagg aaatatatcc
      121 tgccggacgt gacctcggac ggggcggtga tgttcgtgcc ggcggaggcg gtgttcgccg
      181 aaatccacgc ctatcatgcc gaggtcgtgg acgacgccaa tgcaaaacgc gtctggatcg
      241 tgtcgccgac cacgctgatg gcggtgctga acaccgcgcg ggcggtgctg aaagacgtcg
      301 aaacccgcaa acagatccat gtcatccgcg aagcgctgaa gcgcctcgcg gtggagttca
      361 accgcttcga cgagcggatg aaggacctgg cgcgccacat caggcaggcg gccgacgacg
      421 cggaccggat ccaggtcacc ggcggcaaga tctcccagca gttccagcgc atcgagtcgg
      481 ccgagctgga gccgtcggat accgcccagc tcaaggtggt tgaattgcgg gacgacggca
      541 agggggccaa gtgacagggg cccgatcggt cagccgccgc ccccgctccc gggccggggg
      601 cccggcgcgg cacggtccag cgcgccggag atgaaacagg gccgcgtcaa ccatgcagtg
      661 gttcaggaac tggcgccggg aaagaatcct caagcgctcc actctcgacg agaggctgtg
      721 gcgcgtggtg ctcgggcgct atccctttac ccgtgcgctg tccgcagaag aacgtgcgcg
      781 gttacgagac tgggtgatcc tgttcctgca cgataagtcc atcgtaggcg cgggcggcct
      841 cgcgctgcgt gacgacatgc gcatgtgcat cgcggcccag gcctgcatgc tgatcctcaa
      901 tctggatctc gacttctacc gcggctggct ggaggtgatc gtctacccgg gcgaattcgt
      961 cgccgagtat gagtacatgg acgaggacgg cgtcgcgcat cacgtcgaag agccgatgag
     1021 cggggaatcc tggctcgcgg ggccggtgat cctgtcgtgg gcggacaccg agctgcgcgg
     1081 ggacgagcac ggttacaacg tggtgatcca cgagttcgcg cacaagctcg acatgctgaa
     1141 cggcgacgcc aatggttttc cgccattgca cgccggcatg gaccggggcg agtggtcacg
     1201 ggccttcagc gcggcctatg agcacttccg gggccgcgtg gagcggggag ggccgaccgg
     1261 aatcgatcct tatgccgccg aaaatccatc cgagttcttc gcggtgctga gcgaggcgtt
     1321 tttcgagatc cccgacatcg tgctcgccga ctacggcgag gtctaccgac aactggcggc
     1381 cttctatcgc caggatcccg cggcgcgccg cgagagccgg gccgcgaaga cggcagggca
     1441 ggtcgccgcg ctatgacgcc ctcgctcgcc gacaccaagt ccagaacgct gctgctcatc
     1501 agcttcggac tgctgctctc ggtgctcacc gcgttgatcg cgctcgggtt ggcacgcatc
     1561 gagtcgttca accagcagat caacgcgctc accgaggtac agggccgcag gatcggcgtg
     1621 atttcggagg tctttctcgc caacggccag cgatcggcgc tgatcgaccg gttgtttgcc
     1681 gcggaatcgc gacaggcgcg gcaggccatg ctcacccagt atcaggcggc cgtcgcagct
     1741 tatacccggg cggtcgggaa attgcgggtg ctggaggtcg ccgccgccgc gcacgcagcg
     1801 cgcgacgagg cgattgcggc ggcagggacc gctctggcac tcggcgaccg gatcgttgcg
     1861 ctgttgatgc ggggcgagat cgcgaccgcc tcggagctca atctcaccca ggcggtcatg
     1921 gtcgacagcc ggctgcagga aacgctttat ctcctgctgg aggccaatca tgcccagacg
     1981 gcgcagtcga tcgccgccgc caaccagggc atgcgaaacg gttttctgct gatcggcgcg
     2041 ggcggagtcc tcgcgctgat cgccggcatc gtgatcgcgg tgctggtgat ccgcatcgtg
     2101 tccacgaccg aggcgcgtct cgagcacgag aaggagctcg cagaagtcac tcttcactcg
     2161 atcatggacg gggtgatcac caccgatgcc aaaggccgca tcgagtatct gaacccggtg
     2221 gcggagcatt atctgggctg gtccagcgca caggcggccg gcagacggct gcccgaggtc
     2281 tttcgcatcg tcgacgagcg cactgggagc gcgatcgaga cgctgccgtt gaccgcagga
     2341 catggcgcgg gagagacgga gccgatcgcg gtgcggctga tcgaccgcag cggacgagag
     2401 tgcccggtgc gttactcgca cgccccgatc cgcgggcgcg acggaacggt gcacggcatg
     2461 atcgtggtgt tccacgacgt gagccagatc cgcgccatgg cccagcagct catctggcag
     2521 gcgagccatg atgcgctcac cggcctggtc aaccgccgtg aattcgagcg gcggctcttc
     2581 gagttgatcg agaccgccag gatgcagcga cgcgagcacg ccctcctgtt catggacctg
     2641 gacaacttca aggcggtgaa cgacacctgc gggcacagcg cgggagacga gctcctgcgc
     2701 cagttgaccg cgatcatgct ttcgcgcatg cgcggcagcg acacgctcgc gcgcctgggc
     2761 ggcgacgagt ttggcgcgct gctggaggcg tgcccccttg agcaggcgct gcgcatcgcc
     2821 aatgccatgc gcgagacggt gcgcgagttc cgcttcgtgt gggaggacaa gaccttcaac
     2881 gtcggggtga gcatcgggct cgtcccgatc aatgcggcga gcggcgacgt caaccaggtg
     2941 ctcgccgccg ccgacgcttg ctgctatgag gcgaagaacc gcgggcggga ccgcgtgcag
     3001 gtccatcgcc cgcacgatcc cgactacagc agccgccacg aggagctgca gctcgtgtcc
     3061 cagatcaccc acgcgttcga gcacggccgg ttccgcctct accgccagcg catcgcaccg
     3121 ctcgaccgtg cgccgcgccg cgagccgcat tacgaggtgc tggtgcgcat gctggaccgc
     3181 tccggcaacc cggtgccggc caccgggttc atgccggcgg cggaacgtta caacctgctc
     3241 acctcgatcg agcgctgggt ggtgagctcg ctggtggagt tcctgagccg gcagtgggcg
     3301 gcgggcgcga tcccccacga cctgcacggc ggcgaagagc gcggcttcta ctcggtcaac
     3361 atctcggggg cgagcatcaa cgacaagagc ttcccggatt tcctgcgcaa tctcctgacc
     3421 cgctaccagt tgccgagcgg attgctgtgc ttcgagatca cggagaccac ggcgatctcg
     3481 aatctttcca aggcggcgga actgatgcac gagctgaagg ggatgggctg ccgctttgcg
     3541 ctcgatgact tcggcaccgg catgtcctcg ttcgcctatc tcaaatacct gccggtcgat
     3601 ttcctcaaga tcgccggcat cttcatcaag gacatggtga ccgatcccat ggactacgcc
     3661 atcgtggatt ccatcaatcg catcagccac atactgggca tgcgcacggt ggccgagtcc
     3721 gtggaggacg ccgcgacgct cacgcgcatc accgagctcg ggctcgacta tgcccagggt
     3781 tatttcatcg cggagccgga gctgctgggt gacgagccgg cgcagcggca gaccgcgctg
     3841 ttcgc
//
LOCUS       DBNGWZ010000363         4952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3192340, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000363 DBNGWZ010000000
VERSION     DBNGWZ010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3192340"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1394
                     /gene="nirJ"
                     /locus_tag="AC16CX_10315"
     CDS             189..1394
                     /gene="nirJ"
                     /locus_tag="AC16CX_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HIR3012940.1"
                     /translation="MFRISQFMKEMAHPTSRAPRRDPSGPVVIWNLIRRCNLACRHCY
                     SISADTDFPGELTTAEVFAVMEDLKRFSVPVLILSGGEPLLRRDLYEIADHAKHMGFY
                     VGLSSNGTLIDESNIGRIAAVGFDYVGISLDGIEGTHDHFRRMPGAYRRSLNALRLAR
                     DAGIKVGVRFTLTQENHHDLPALLALIERERIDKFYLSHLNYAGRGNVNRGTDAYHET
                     TRRAMDLLFDTCWSYVERGVEKEFVTGNNDADGVYLLFWVRRRFPRWEGDLRVRLAQW
                     GGNASGVNVANIDNLGNVHPDTMWWHYSLGNIRERPFSEIWSDTSDPLIAGLKARPRR
                     VGGRCAACAYFDVCGGNTRVRAEQLTGDPWQEDPACYLTDEEIGVSAGRARLRVTPYS
                     RRREREPLS"
     gene            1467..3020
                     /locus_tag="AC16CX_10320"
     CDS             1467..3020
                     /locus_tag="AC16CX_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR3012941.1"
                     /translation="MSHAFLRWLLVALLSAAGGVLAQPRPDRLYAEHCAACHGADRLG
                     AMGPALLPENLARLRRQDAVEVILQGRPATQMPGFSGKFGVEEARALAEHIYAPLAAV
                     PQWGEAEIRASRIEHVRPETLPRKPVFKADPLNLFVVVETGDHHVTILDGDRFEPIHR
                     FPSRFALHGGPKFTPDGRYVFFASRDGWISKYDLYGLKTVAEIRAGINTRNLAVSGDG
                     RFVMVANYLPHSLVLLDAGLNLIKVLEVRDRDGKRTSRVSAVYDAAPRKSFVAALKDI
                     PEVWEVSYDEKAEPIPAGLIHDFRLQEGAFIPGRFNPRRSVLAEHLDDFFFTPDYTQL
                     MATSRDGGEGQVVHLDVRRKIATVPVPGMPHLASGITWNYQGRTVMATPNLKEGVVTV
                     IDIGSWRTVKEIRTLGPGFFLRSHEASRYAWVDAMMSPHRDTLQVIDKQTLEIAAELR
                     PVPGKTLAHVEFTRDGRYALASLWENDGALIVFDARTLKEVKRIPMTKPSGKYNVHNK
                     ITRSEGTSH"
     gene            3046..3690
                     /locus_tag="AC16CX_10325"
     CDS             3046..3690
                     /locus_tag="AC16CX_10325"
                     /inference="COORDINATES: protein motif:HMM:NF025244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HIR3012942.1"
                     /translation="MTADRGLPVIADPERFERALARLDAANALDPNREIVNGVAEPKE
                     LAYARRMTEMLERFAPEASEPVRLAVRAQHIRRWEIPRATYPRTPAGYRQWRTRLLAF
                     HAEAAAEILRAEGYDESVIERVGTLLRKEGIKRDPDVQLLEDVAGLVFLEHYLADFAR
                     RHPEYDEAKLIDILRKTLKKMSARARAAAGGLIRAPAAVTLLLLKAAGAADPES"
     gene            complement(3715..4407)
                     /locus_tag="AC16CX_10330"
     CDS             complement(3715..4407)
                     /locus_tag="AC16CX_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3012943.1"
                     /translation="MPTTPIATQTFLANIPLFKELAPEELDRIAAGTRQVHVARGDVL
                     FRRGDPCAGFHAVVYGQIKLAFTSPEGAEKVIEIIGPGQSFGEAVMFVEKPYPVYSQA
                     LADSLLLFIAKSVVFAETERDPKLARKMISGLSRRLHGLVTDLESYTLHSGTQRVIGY
                     LLRGVPDGEPASGPVRISLATSKTVIASRLNLTPEHFSRILHDLTQDGLIEVEGRSIS
                     ILDVEALRRYGG"
BASE COUNT          844 a         1618 c         1709 g          781 t
ORIGIN      
        1 atggcaggac cagcggcgaa gtcgagtcga aagtgcgcga gatcgcgacg atcgtcggcg
       61 atgccgaccg gggtcacgcg gtgctctaca gcacgcgcat cctcaagaag accggccttc
      121 gcctgacggc gagtgacgag taatgagtgt cgagggacga gtaacgagaa acgagtagcg
      181 gggaccggat gttcaggatt tcgcaattca tgaaggaaat ggctcacccg acgtcgcgcg
      241 cgccgcggcg ggatccatcc ggccctgtcg tgatctggaa cctgatccgg cgctgcaatc
      301 ttgcctgccg ccattgctac tcgatttccg cggacaccga tttccccggg gaactcacca
      361 ccgcggaagt gttcgccgtg atggaggacc tcaagcgatt cagcgtgccg gtcctgatcc
      421 tgtcgggggg cgagccgctg ctgcgtcgcg atctctacga aatcgctgac cacgccaagc
      481 acatgggttt ctacgtgggc ctgtcgagca acggcacgct gatcgacgaa tccaacatcg
      541 gccgcatcgc ggcggtggga ttcgactatg tcggcatcag tcttgacggc atcgagggca
      601 ctcacgatca tttccgccgc atgccgggcg cctatcggcg ctcgctcaac gcgctgcgcc
      661 ttgcacgcga cgcgggcatc aaggtcggcg tgcgcttcac gcttacccag gagaatcacc
      721 acgacctgcc ggccctgctg gcgctgatcg agcgcgagcg gatcgacaag ttctacctgt
      781 cgcacctcaa ttacgcgggc cgcggcaacg tgaaccgcgg gaccgacgcc taccacgaaa
      841 ccacgcggcg cgcgatggac ctgctgttcg acacttgctg gagttacgtc gagcgcggcg
      901 tcgagaagga attcgtcacc ggcaacaacg atgcggatgg ggtctatctg ctgttctggg
      961 tacgacggcg tttcccgcgc tgggagggcg acctgcgggt gcggctcgcg cagtggggtg
     1021 gcaatgcgtc gggcgtcaac gtggcgaaca tcgacaacct cggcaacgta catccggaca
     1081 cgatgtggtg gcactacagc cttggcaaca tccgcgagcg gccgttttcc gaaatctgga
     1141 gcgacacctc cgatccgctg atcgcggggc tcaaggcgcg gccgcgccgg gtcggtgggc
     1201 gttgcgcggc gtgcgcgtat ttcgacgtct gcggcggcaa cacgcgggtg cgcgccgagc
     1261 agctgaccgg cgatccctgg caggaggatc ctgcgtgtta tctaacggat gaggaaatcg
     1321 gcgtgtccgc ggggcgcgcg cgattgcggg tgacgccgta ctctcgccgg cgcgagcgcg
     1381 aacctttgag ctgaagcgtg tcctagccgc agaggtcacg gaaaacacag atcggcgtga
     1441 agccacgacg gatgactttt cattcgatgt cgcatgcgtt ccttcggtgg ctgctggtgg
     1501 cgctgctctc ggcggcgggc ggggtgctcg cgcagccgcg cccggaccgg ctctacgccg
     1561 agcactgcgc ggcgtgccac ggcgccgatc ggcttggcgc gatggggccc gcgctgctgc
     1621 cggagaacct ggcacggctg cggcgtcagg acgccgtgga ggtcattctc cagggacggc
     1681 ccgccacgca gatgccgggc ttttcgggga agtttggcgt agaggaggcg cgggcgctcg
     1741 cggagcacat ctacgcgccg ctcgcggccg ttccccagtg gggcgaggcg gaaatccggg
     1801 cttcgcgcat cgagcacgtc cggccggaga cgctgccgcg caaaccggtg ttcaaggccg
     1861 atccgctcaa cctcttcgtc gtggtggaga ccggcgacca ccacgtcacc atcctcgacg
     1921 gcgatcgctt cgagccgatc caccgctttc cctcgcgctt cgcgttgcac ggcgggccga
     1981 agttcacgcc cgacggccgt tacgtgttct tcgcctcgcg cgacggatgg atctccaagt
     2041 acgatctcta cggcctgaag acggtcgccg aaatccgcgc cggcatcaac acccgcaatc
     2101 tcgccgtatc gggcgacggc cggttcgtga tggtggcgaa ctacctgccg cacagcctgg
     2161 tgctgctcga tgccggcttg aacctcatca aggtgctgga ggtgcgcgac cgcgacggca
     2221 aacgcacctc gcgcgtgtcc gcggtgtacg acgccgcgcc gcggaagagc ttcgtggcgg
     2281 cgctcaagga catccccgag gtgtgggagg tgagctacga cgagaaggcg gagccgatcc
     2341 ccgccggtct catccacgac ttccgccttc aagagggcgc attcatcccc ggccgcttca
     2401 atccgcggcg cagcgtgctc gccgagcacc tcgacgactt cttcttcact cccgactaca
     2461 cccagttgat ggccacctcg cgcgatggcg gcgagggaca ggtggtccac ctcgacgtgc
     2521 ggcgcaagat cgcgacggtg ccggtgccgg gcatgccgca tctcgcctcc ggcatcacct
     2581 ggaactacca gggccgcacc gtgatggcga cgcccaacct caaggagggc gtcgtcaccg
     2641 tcatcgacat cggcagctgg cgcacggtca aggagatccg caccctcgga ccgggtttct
     2701 tcctgcgcag ccacgaggcg agccgctacg cctgggtgga cgcgatgatg agcccgcacc
     2761 gcgacacgct gcaggtgatc gacaagcaga cgctcgagat cgcggccgag ctgcggccgg
     2821 tccccggcaa gacgctcgcg cacgtcgagt tcacgcgcga cgggcgctac gcgctcgcca
     2881 gcctgtggga aaatgacggc gcgctgatcg tgttcgatgc gaggacattg aaagaagtga
     2941 agcgcattcc catgaccaag ccctcgggca agtacaacgt ccacaacaag atcacccgct
     3001 ccgagggcac cagccattga cgcctctcgc gtgggagcaa ccaccgtgac ggcggatcgc
     3061 ggcctgccgg tgatcgcgga ccccgagcgc ttcgagcgcg ccctcgcgcg cctggatgcg
     3121 gccaacgcgc tcgaccccaa ccgcgagatc gtgaacggag tggccgagcc gaaggagctc
     3181 gcgtacgcaa ggcgcatgac cgagatgctc gagcggttcg cgccggaagc gagcgagccg
     3241 gtgcggctgg cggtgcgggc ccagcacatc cggcgctggg agatcccgcg cgcaacctat
     3301 ccgcgcactc cggccggcta ccggcaatgg cgcacgcgcc tgctcgcgtt ccacgccgag
     3361 gcggccgcgg aaatcctgcg cgcggagggc tatgacgaaa gcgtgatcga acgggtcgga
     3421 acgctcctga ggaaggaggg catcaagcgc gacccggacg tgcagttgct ggaggacgtc
     3481 gcggggctgg tgtttctgga gcactacctg gccgatttcg cgcggcggca tcccgagtac
     3541 gacgaggcca agctgatcga catcctgcgc aagacgctca agaagatgtc cgcgcgcgca
     3601 cgcgcggccg ccggcgggct gatcagggct ccagcggcgg tgacgctcct tctgctcaag
     3661 gcggccggcg cggcggatcc ggaatcctga ggatcgggcc cggccgcccg cggctcagcc
     3721 gccgtagcgc cgcaacgctt cgacgtcgag aatgctgatg ctgcgcccct cgacctcgat
     3781 caggccgtcc tgcgtgaggt cgtgcaggat gcgcgagaaa tgctccggcg tgaggttcag
     3841 gcgcgaggcg atgacggtct tgctggtcgc gagcgagatc cgcaccggcc cgctcgccgg
     3901 ctctccgtcc ggcacgccgc gcagcaggta gccgatcacc cgctgcgtgc cggagtgcag
     3961 cgtgtaggac tcgaggtcgg tcaccaggcc gtgcaggcgg cggctcaggc cgctgatcat
     4021 cttgcgcgca agcttcggat cgcgctcggt ctcggcgaac accacggatt tggcgatgaa
     4081 gagcagcagc gagtcggcca gcgcctggga gtagaccggg tagggcttct cgacgaacat
     4141 gaccgcctcc ccgaaactct ggccgggccc gatgatctcg atcacctttt ccgcgccctc
     4201 cggcgaggtg aaggcgagct tgatctggcc gtagaccacc gcgtgaaagc ccgcgcacgg
     4261 atccccccgc cggaacagga cgtcgccgcg cgcgacgtgg acctgccgcg ttccggcggc
     4321 gatgcggtcg agttcctccg gtgcgagctc cttgaacaac ggaatgttgg cgagaaaggt
     4381 ctgggtcgct atcggcgtgg tcggcaagtc cgctctcctg ccgggtggtt gcggcgccgg
     4441 ccattatccc atcaacccgg gacccccggc gggtcgcccc ggccggggtg cgattgccgg
     4501 cgcgccgtag aatgtcgctg cggccgccgg gacagggcgg cgcggaaggc gaaaaacggg
     4561 ccggctagca gcctgtcgaa ctttaacggt ccgcacaggc tgctaacagc aaaaccggct
     4621 gacccttcag caaaaaaccc ggccgacggg gtcgaatgta tggaaaagcc aaggtgattt
     4681 tgcatatccg tcatcctctc tggcaatttt cctggtgtcg gttttgcatc aggagtttgt
     4741 cggggccagc gccagcggag ggcggagcag gttccaagcg atttgtgctt ggatggcgcg
     4801 tcgtagggcg gaagaggggc cccacagggg atctgacccc cgagacgcgc atagggccgg
     4861 atgcgacgcg tgcacggccg ggtccacggt cgcatcccgc cagcggggcg gcggacgagg
     4921 gggggcaggg gccccggtgc cgttcggggc gg
//
LOCUS       DBNGWZ010000364         1835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_44523, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000364 DBNGWZ010000000
VERSION     DBNGWZ010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_44523"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16CX_10335"
     CDS             complement(<1..218)
                     /locus_tag="AC16CX_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3012944.1"
                     /translation="MKYAVLMSAACACMVCQAADRTDYPARPVRVIIPQGPGSTTDLL
                     GRIAFTHTADRLGKQLVVDNRPGAGGTLG"
     gene            333..1247
                     /locus_tag="AC16CX_10340"
     CDS             333..1247
                     /locus_tag="AC16CX_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012945.1"
                     /translation="MSTVAGSTKLQYVYDFATLDTMPVAPTSARVAARRLLSGPSLMT
                     GKSSTVGAVLSGERIILTLGTQARGSGAKPHTHPNEQFNYILQGTMVNEIEGELVFAG
                     PGTLLHTPTLAVHTGLACPDEDLVFLAMKDTRHGIVGPPVDGQYTGPNYLPGFGTRAG
                     EPMKTTAQLIAGAGRDPAGEKVRYVYDFANLAEKPGRVSKVRVAPAGPMPRSGATGTV
                     LTGELLHIAVLRYPKGAAVSVNSSPAECFVFVVSGSLRGEVDGQAVMVRQRCIAHVPT
                     GVPHGFAAAGDGDTLVVIAQDNRHAFIA"
     gene            complement(1350..>1835)
                     /locus_tag="AC16CX_10345"
     CDS             complement(1350..>1835)
                     /locus_tag="AC16CX_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012946.1"
                     /translation="ASHFAGLLFAKAAGIEILHVPYKGGAPAIAAVLAGEAAFNFGPM
                     PATAMHIKSGRLKALAVSGATRSNAFPSVPTVAESGVPGYVVVGWFGLMAPKGTPKAI
                     VERLHAATVKAVNSPDTRSQLINVGADPAPNSPDEFGKFVRREYERYGKLIKEAGLKV
                     E"
BASE COUNT          298 a          612 c          630 g          295 t
ORIGIN      
        1 cccagcgtgc cgccggcgcc cggacggttg tccacgacca gctgtttgcc gagccggtcg
       61 gcggtgtgcg tgaacgcgat ccggcccaat agatcggtgg tgctgcccgg cccttgggga
      121 atgatcacgc gcacgggccg cgccggatag tcggtccgat cggctgcctg gcacaccatg
      181 cacgcgcagg cggccgacat caacaccgcg tatttcatga tccctcctcg ttgtgacctg
      241 cgtccatata gtgtctcatg gcgccgcgcc gcgtggcagg ccggcgatgc tggtggtaga
      301 ttagcggcgt ccacgactcc agcgcattca acatgagcac ggtcgccggg agcaccaagc
      361 tccagtacgt ttacgatttc gcgacgctcg acacgatgcc ggtcgcgccg accagcgccc
      421 gcgtcgcggc ccggcggctg ctgtccggcc cgtcactgat gaccggcaag tcgtccacgg
      481 tcggcgcggt tctcagcggc gaacgcatca tcctcacgct cggcacgcag gcccgtggct
      541 cgggcgcgaa gccgcacacg caccccaacg agcaattcaa ttacatcctc cagggcacca
      601 tggtcaacga gatcgagggc gagctggtgt tcgcgggacc gggaacgctc ctgcacacgc
      661 cgacgctcgc cgtgcacacc gggctcgcgt gcccggacga ggacctggtg ttcctcgcga
      721 tgaaggacac gcgccacggc atcgtcggcc ctcccgtcga cggccaatac accgggccga
      781 actatctgcc cgggttcggc acccgcgctg gcgagccgat gaagacgacg gcccaattga
      841 tcgccggagc ggggcgcgat cctgccgggg agaaagtgcg ctacgtctac gatttcgcca
      901 acctggcgga aaagccgggc cgggtatcca aggtgagagt ggcgcctgcg gggccgatgc
      961 cgcggtcagg cgcaaccggg actgtcttga ccggcgaact gctgcatatc gcggtgctgc
     1021 gctatccgaa aggtgcggcg gtgagcgtga actcgagccc cgccgagtgc ttcgtgttcg
     1081 tggtctccgg atcgctgcgc ggcgaagtgg acggccaggc tgtgatggtc aggcagcgct
     1141 gcatcgcgca cgtgccgacc ggcgtcccgc acggtttcgc ggcggcgggc gacggcgaca
     1201 cgctggtggt gatcgcgcag gacaatcgcc acgcctttat cgcgtaggcg agggacggag
     1261 tgtcgaggat tgagtgtcga ggagacaaac gaggaacggg gatggaaggt cgagggggta
     1321 accactcgtc actcgacacc cgacacttgt cactcgacct tgagtcccgc ttccttgatg
     1381 agcttgccat agcgctcgta ttcgcgccgc acgaacttgc cgaactcgtc gggcgagttg
     1441 ggcgcggggt ccgctccgac gttgatcaac tggctccggg tatcgggact gttgacggcc
     1501 ttcaccgtgg cggcgtgcag gcgctccacg atcgccttcg gcgtgccttt cggcgccatc
     1561 aggccgaacc agcccaccac cacgtagccg ggcacgccgg actcggccac cgtcggcacg
     1621 ctgggaaatg cgttcgagcg cgtggcgccg ctcaccgcga gcgccttcag ccggcccgac
     1681 ttgatgtgca tggccgtggc gggcatcggg ccgaagttga acgccgcctc gccggcgagg
     1741 accgcggcaa tagccggtgc gccgcccttg tagggcacgt gcaggatctc gattccggcg
     1801 gccttggcga agagcaggcc cgcgaagtgg ctcgc
//
LOCUS       DBNGWZ010000365        12115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_419807, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000365 DBNGWZ010000000
VERSION     DBNGWZ010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_419807"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1013)
                     /locus_tag="AC16CX_10350"
     CDS             complement(186..1013)
                     /locus_tag="AC16CX_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3012947.1"
                     /translation="MAELVGVYAASHGPMIVRNWPIIPPARKDTLTSAFSELGRRIRS
                     ARPDVLIVISPDHWVNFFIDNLPGICVGVGETHEGPPEPWLKEFPHRSLAGHPQLAGH
                     IVAAAFERDFEPSVSYRLALDHGFCIPLWKAGLDPLPPVIPVVFNDLEPPFPAVKRCF
                     AWGNVLAEAVATYPGSLRVAILATGGLSHSIGEPTMGEIDEAFDRECIRHFENGNPHA
                     LFDFLDQRLPHAGNGASEVRNWVAAHGAAHGRGFELIRYAAIPEVYVGCGFASWKVG"
     gene            complement(1006..1284)
                     /locus_tag="AC16CX_10355"
     CDS             complement(1006..1284)
                     /locus_tag="AC16CX_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012948.1"
                     /translation="MRDYWLSKLFFDLQSPPVADEYRADRKKVLGRYPLKPEVRAAVE
                     ADDVAYLSALVNPYLLRFYFHMAGMPEEEFLRRIRSAGARAATKAAHG"
     gene            complement(1495..2157)
                     /locus_tag="AC16CX_10360"
     CDS             complement(1495..2157)
                     /locus_tag="AC16CX_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3012949.1"
                     /translation="MPRYTLRLHTDRLEPKAEGRLPPCNRVIYVRDGEAIVRAGGQAA
                     GLAASSAWHGRGEVAVTAGATGTTLLRWELVADDRSTGELAGAGVTSTLTLAQAIELG
                     DPGGYLMRCDRVDFPLGGIAYTHVHRGPGIRCLLTGGIRVEVNGKSHDIAPGGAWFEA
                     GPDPVLAIASKTELAAFARVMILPRELRGKSSIRYVKPEDADKPKLQTYRVFVDEPIE
                     LR"
     gene            complement(2255..3223)
                     /locus_tag="AC16CX_10365"
     CDS             complement(2255..3223)
                     /locus_tag="AC16CX_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012950.1"
                     /translation="MLHTILRLAAAIILAGFAATVSAQSYPTKPIRLLVPFGPGGVGD
                     ITARVVAQKMSASLGQQVIIDNRPGAGGIVASELVAKAEPDGYTMLLLNNAHAISMSL
                     FKSLPYNTLRDFAPVSSLATFSIVLLVNPDSPIKSVKDLIASAKASPGKLNIGTIQIG
                     ATQHLSSELFKSMAGVDMVHVPFSNTGAVLTGLRGGSVQIAFEFIAPVVGQVKGGALR
                     ALAVTTRNRFSGLPDIPTVHEAGVRGYEVTSWNGIGVPAKTPRAIIDRLNRAMAEALV
                     ASDVKQRFQELAVEARPDTPEGFRKLIASEIAKWRKVIENAGIPKQ"
     gene            3350..4216
                     /locus_tag="AC16CX_10370"
     CDS             3350..4216
                     /locus_tag="AC16CX_10370"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA dioxygenase family protein"
                     /protein_id="HIR3012951.1"
                     /translation="MALKLRKLSYALGAEVCDVDVAKPMGERQFGEIYKAFLDDNILL
                     FRNQDITRSQHIEFSRRFGELDRHDALPRDRHPDYPEILMVTNEPKPDGSPSDTQYTG
                     RQWHSDLSFTTAPSLGSLLRCYECPDVGGDTLFANMYLAYDALSEGMKKLIADLHGIH
                     LSGTRKIANAETGVPRAEEQRRINPPVAQPVVRVHPETGRKALYIGEKVKRFDGMAEE
                     ESKPLIDYLVRHATRPEFVYRHQWKKNDILVWDNRCTLHQALGDFDETRLRHMERTTV
                     LGTPSGYVVANG"
     gene            4267..4665
                     /locus_tag="AC16CX_10375"
     CDS             4267..4665
                     /locus_tag="AC16CX_10375"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3012952.1"
                     /translation="MSSIVRQSVTLPAPSRELYAMYLSASRHGAITGGKVVISARHGS
                     RFSAFDGMLRGRTLHTVPGRLIVQAWRSMGWRKSDLDSTLILRFIDKGRSGRIDLVHV
                     NVPDHDYRGVNRGWKQYYWRPWRKYLARIR"
     gene            4671..5789
                     /locus_tag="AC16CX_10380"
     CDS             4671..5789
                     /locus_tag="AC16CX_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3012953.1"
                     /translation="MTRLSPSARSAAADDAIVTIDHIVPHVSTVPANLGQTVGLFVRE
                     KLPAGTQRPARPRVALFIHGGYSPAVVAYDLDYMGFSFMAALARAGFDVFAMTHTGYG
                     ASPKPMMDDPCNVDAAFQPLIIPHVLKAPCPPRYPFKLVSSRTEWDEIAAVVDFIREL
                     RGVERVSLIGWSTGAPRAGGFAALHPDQVDKLVLFGPSPFFAGDDPPAQMPEPGAPVI
                     LQTKEMLLEKRWRNDVRCDGQVDDPEVREVMWRELIRQDGLGAHWRPEGAMRAPNRMN
                     YGWRRNCAAIRAPTLVLLGEFDNYRQRRDAWKGLGVDRRALIKVACASHFMQYERGRH
                     VLHRATREWLETGSIEGMKHAEFHADAEGRLTKLREAG"
     gene            complement(5941..7560)
                     /locus_tag="AC16CX_10385"
     CDS             complement(5941..7560)
                     /locus_tag="AC16CX_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3012954.1"
                     /translation="MTAQLKAWYEPLTPLSFLERVAHVMPDRMAVIDKDRCWTWKEFF
                     GRVNRLSNALKGLGVERGDNVAFLSRNFPPLLEAHYGIPLTGGAIVAINYRLSAPEIA
                     TIVNLSEARVMFVDAGVADRVNPDALPKVETYINVFDGRDTYGETPTKEMPGVGYEEF
                     IAGASDEYVRLELKDENEVIAIDYTSGTTGNPKGCIYSHRSAYLHAIAKIIEHSVNAY
                     SVYLWTLPMFHCNGWCWTWVTSGVGATNVCLPVTDPDRIWRLIEKHGVTHMAGAPIVF
                     HRLGRYMDENGIDKFPNKLIMNVAAAPPPANMLRSMEVKGGEIRHAYGLTETYGPFTI
                     CEWHPEWDALPDDQRLGLKMRQGVPDITAGYIRIVDGDGNDVPRDGKTIGEIIMRGND
                     VVQGYHKAPQENAKAFRGGWFYSGDGGVFHPDGYVEVKDRFKDVIISGGENIIGVEVE
                     NCIYQHPDVNEVVCFGKPDETWGEVVKCLVHPKPGTSPTEEEIIAFCRDRLAHFKCPK
                     EIEFGEIPRTAAGKVQKFMLRNKEKRKADER"
     gene            7896..8882
                     /locus_tag="AC16CX_10390"
     CDS             7896..8882
                     /locus_tag="AC16CX_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012955.1"
                     /translation="MAPVPALIFALCALFAAVSAFAQTAVATYPNKPLRLVHGFPAGG
                     PADIFSRIIAQKLSEVVGQQVIVDSRPGAGSIIATEHVARAPADGYTAYFASSAILAL
                     YPNLYDKLPYDLNRDFVPVTLAVAVPELLVVHPSLPTRTAKEFVALAKANPKQLIYGS
                     TGNGNMPHLAMESFKIAAGVNILHVPYKGAAPAVADLLGGHVHTVILDVPVLLPHVKS
                     GKARALAIATGKRSPALPDVPTMAEAGYPSVNADNWYGIIVAAATPKDVVSRLHASLV
                     AALQAPETRQRMENQGANALSSSPQELAAHMRNETIKWGKIIKTAGIKLDAQ"
     gene            complement(8992..9192)
                     /locus_tag="AC16CX_10395"
     CDS             complement(8992..9192)
                     /locus_tag="AC16CX_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR3012956.1"
                     /translation="MAAKRRVFADCRLYPSEKNCSLYIAGTEEEVFEVALRHAITSHG
                     HKDTPEFREQLRSFLKEEPQSK"
     gene            complement(9402..10244)
                     /locus_tag="AC16CX_10400"
     CDS             complement(9402..10244)
                     /locus_tag="AC16CX_10400"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3012957.1"
                     /translation="MTSDPAALFSRDFIPMPRVVALYRYPVKGFTPEECEALTVLEEG
                     RIAGDRALAFRFADSGAPDDAWAPKQQCVVLMNTPGLARLNVRLNHTALRLRMSLDGA
                     VLAEAVLDVDGRRRLAAALADYVLKLDVNPLAGRAERLPLKLVGDGVTPRYQDNDGGH
                     ATLHSRESLAAVAAATGDPAIDEVRFRSNIAIEGVNAWEEQNWMGRRLRIGAVEFEVV
                     RRKIRCLATHANPRTGERDIAVMPTLMKAFSQKQPTFAIGMVTRGAGGRVRVGDKISV
                     VDQG"
     gene            10319..11383
                     /locus_tag="AC16CX_10405"
     CDS             10319..11383
                     /locus_tag="AC16CX_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3012958.1"
                     /translation="MKQQIRFCTTADGVRIAYATTGEGPPIVKVSNWLSHLEYDRESP
                     VWRHWIAELSRDHTYLRYDERGCGLSDWEVPELSFEAWVRDLETVVDAAGIERFPLLG
                     ISQGASIAIAYTVRHPERVSHLILHGAYARGRLKRNLTPQQCKEAETMAELAELGWGK
                     ENPAFRQFFTTQFIPDGTIEQHNWFNELERVSASPENAARFMRIFDQIDVTDLAPKVS
                     CPTLVLHPNRDARVPFDEGRLFAGLIPGARFVPLESRNHILLENEPAWRQWLDEVRAF
                     LPAESVGGAAFSELTPRERDLVELIAQGLDNAQIAARLSLSEKTVKNHITSIFTKLEV
                     ENRSRAIVLARDAGFGRKPR"
     gene            11548..11916
                     /locus_tag="AC16CX_10410"
     CDS             11548..11916
                     /locus_tag="AC16CX_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012959.1"
                     /translation="MNENRQQNGATPLQSNLLRLSERREIAIYLRDGAAWVADFNDGR
                     GELYTAGAWCGMGGSRMLAHAQRRCEVETISPLPDEVVQRIESLHRRMEKAVVGPALR
                     RALAILVAVFRHPGSSVQPY"
     gene            11929..>12115
                     /locus_tag="AC16CX_10415"
     CDS             11929..>12115
                     /locus_tag="AC16CX_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012960.1"
                     /translation="MQAAQHQIDTKRYAKCVEVSKRIRWDIDRDVIRGRTFDFSRKFL
                     PDGLSLVDKLDFLDANER"
BASE COUNT         2202 a         3882 c         3868 g         2163 t
ORIGIN      
        1 gttgatcgca aagcgaacag acatgctttc cctcctcttt tttatgaatt gcgtgagtcg
       61 tgagtggtga caaagatctt gttcaaagtt ccaagttcca agtcgaaacc ttcgaatcac
      121 agcgcgctcc aaccttgaac cttgaacggg actttcgtcg caactcacga atcacgattc
      181 acgcatcatc caaccttcca ggaggcgaag ccgcagccca cgtacacctc gggaatcgcg
      241 gcgtagcgga tcagctcgaa gccgcgcccg tgcgccgccc cgtgtgcggc gacccagttg
      301 cggacctctg atgcgccgtt gccggcatgg ggcaggcgct ggtcgaggaa gtcgaacagc
      361 gcgtgcggat tcccgttctc gaagtggcgg atgcattcgc ggtcgaacgc ctcgtcgatc
      421 tcgcccatgg tcggctcgcc gatcgagtgg ctgagccccc cggtcgcgag tatcgccacc
      481 cgcaggctcc cgggataggt cgcgacggct tccgcgagca cgttgcccca cgcaaagcag
      541 cgcttgacgg cgggaaacgg cggctcgaga tcgttgaaca cgaccgggat gacagggggc
      601 aggggatcga gcccggcctt ccacagcggt atgcagaatc cgtgatcgag cgcgagccgg
      661 tacgagaccg acggctcgaa atcgcgctcg aaggcggccg cgacgatgtg acccgcgagc
      721 tgcggatgtc ccgcgaggct gcggtgcggg aattccttca accagggctc gggcggtccc
      781 tcgtgcgtct cgcccacgcc gacgcagatg cccggtaggt tgtcgatgaa gaagttcacc
      841 cagtggtcgg gcgaaatcac gatcagcacg tccggccgcg cggagcggat gcgccgcccg
      901 agttccgaga acgccgacgt gagagtgtcc ttccgcgcgg gcgggatgat cggccagttg
      961 cgcacgatca tcgggccgtg gctggccgcg tagacgccga cgagctcagc catgggcggc
     1021 cttcgtcgcg gcgcgcgcgc cggcactgcg gatgcggcgc aggaactcct cctccggcat
     1081 gcctgccatg tggaagtaga acctcagcag gtagggattc accagcgcgg agagataagc
     1141 gacgtcatcg gcctcgaccg ccgcgcgcac ctcgggcttc agcggatagc ggcccagcac
     1201 cttcttgcgg tcggcgcgat actcgtccgc cacgggcggg ctctgcagat cgaaaaacag
     1261 cttggagagc cagtagtcgc gcatttgagt gtcgagtgtc gagtgtcgag tgatgagtga
     1321 tgagtggccg tcagcaaatc cgcgcgctcc attttccaac aaatccgggc tggggtcgat
     1381 gtgcgttggg gaacgaggga caaggcgtga taggtgataa gtgatagttg ataggtgttc
     1441 aaggccggta ttgccggaga ctcttatcac ttatcactcg tcacttatca cgctttatcg
     1501 gagttcgatg ggctcatcca cgaacacgcg gtaggtctgc agcttgggct tgtccgcgtc
     1561 ctcgggcttc acgtagcgga tcgagctttt gccccgcagc tcccgcggca ggatcatgac
     1621 ccgcgcgaag gcggcgagtt cggttttcga ggcgatggca agcaccggat cggggccggc
     1681 ctcgaaccac gcgccgccgg gcgcgatgtc gtggctcttt ccgttcactt ccacgcggat
     1741 tccgccggtg agcaggcagc ggattccggg gccgcggtgc acgtgcgtgt aggcgatgcc
     1801 gcccagcggg aagtccacgc ggtcacagcg catcaggtag ccgcccggat cgccgagctc
     1861 gatggcttga gcgagcgtaa gcgtcgaagt cacgcccgcg ccggccaact cgcccgttga
     1921 gcggtcgtcg gcgacaagtt cccagcgcag cagcgttgtg cccgtggcgc ccgcggttac
     1981 tgcaacttcg ccccgtccgt gccaggcgct gctcgcggcg agcccggccg cctgcccgcc
     2041 ggcgcgcacg atcgcttcgc cgtcgcgcac gtagatgacg cggttgcatg ggggcaaccg
     2101 tccttccgcc ttcggttcga ggcggtcggt gtgcaagcgc aacgtgtagc gcggcatggc
     2161 ctggacagag cgtgataggt gataagtgat agatgtgtaa agaggcccgc tgcgcgggag
     2221 acttatcact tatcactcgt cacttatcac gcatttactg cttgggaatg ccggcgttct
     2281 cgatcacctt cctccacttg gcgatctcgg acgcgatcag cttgcggaag ccctcgggcg
     2341 tgtccggacg cgcttccacg gcgagttcct ggaagcgctg cttgacgtcg gacgcgacga
     2401 gcgcctcggc catcgcccgg ttgaggcgat cgatgatcgc gcgcggcgtc ttggccggca
     2461 caccgatgcc gttccaggac gtgacctcgt atccgcggac accggcctcg tgcaccgtcg
     2521 ggatgtccgg caggcccgag aagcggttgc gcgtggtgac ggccagcgcc ctcagggcgc
     2581 cccccttcac ctgtcccacg accggcgcga tgaactcgaa cgcgatctgc acgctcccgc
     2641 cgcgcagtcc cgtcagcacc gcgcccgtgt tgctgaaggg aacgtgcacc atgtcgacgc
     2701 cggccatgga cttgaacagc tccgaggaaa gatgctgggt ggcaccgatc tggatggtgc
     2761 caatgttgag cttgcccggg ctcgccttgg cggacgctat caggtccttc acggacttga
     2821 tgggcgaatc cgggttcacc agcagcacga tgctgaacgt ggccagggac gacaccggcg
     2881 cgaagtccct gagcgtgttg taggggagag acttgaacag cgacatgctt atcgcgtgcg
     2941 cgttgttgag cagcagcatc gtgtagccgt cgggctcggc ctttgccacc agctccgacg
     3001 cgacgattcc gccggcgcct ggccggttgt cgatgatcac ctgctgtccc agcgaggcgc
     3061 tcatcttctg cgcgaccacc cgcgccgtga tgtctcccac gccgcccggg ccgaacggca
     3121 ccagcaggcg gatcggcttc gtgggatacg actgtgccga tacggttgcg gcgaaacccg
     3181 cgaggatgat ggcggccgcg agtcgcaata tcgtgtgcaa catgcctcct ccttcttgac
     3241 ccgtttgagc gaagttaacg cagcaggcgg ggcgggtcaa accgtccccg gctaccgcag
     3301 ccggcagcgg cggggatatg attacgacag gaaccgggag gaggtctcga tggcgctcaa
     3361 gctccgcaag ctctcgtacg cactcggcgc ggaggtgtgc gacgtggacg tcgcgaagcc
     3421 gatgggcgag cggcagttcg gcgagatcta caaggcgttt ctggacgaca acatcctgct
     3481 gttccggaac caggacatca cgcgcagcca gcacatcgag ttcagccgtc gcttcggcga
     3541 gctcgaccgg cacgacgcgc tgccgcgcga ccggcacccg gactatccgg aaatcctgat
     3601 ggtcaccaac gagcccaagc ccgacggcag tccctccgac acccagtaca ccggccggca
     3661 atggcactcc gacctgtcgt tcaccacggc gccttccctg ggctccctgc tgcgctgcta
     3721 cgagtgtccg gacgtaggcg gggacacgct gttcgccaac atgtatctgg cctacgacgc
     3781 gctctccgag gggatgaaga agctgatcgc cgatctgcat ggaatccacc tctcggggac
     3841 gcgcaagatc gcgaacgccg agaccggcgt cccccgcgcc gaggaacaga ggcgcatcaa
     3901 cccgccggta gcgcagccgg tagtgcgggt gcatccggaa accgggcgca aggcgctcta
     3961 tatcggcgag aaggtgaagc gcttcgatgg catggcggag gaggagagca agccactcat
     4021 cgattacctg gtgcgccacg ccacgcgccc ggaattcgtc tatcgccatc agtggaagaa
     4081 gaacgacatc ctggtgtggg acaaccgctg caccctgcac caggcgctcg gcgacttcga
     4141 cgagacccgg ctgcgccaca tggagcgcac caccgtgctc ggcacgccgt ctggttacgt
     4201 ggtggcaaac ggatagagca acaagcggcc cggacttcga acaggatccg aaccagggag
     4261 accgtcatgt ccagcattgt cagacaatcg gttacgctgc cggccccatc gagggagttg
     4321 tacgcgatgt acctcagcgc gagcaggcac ggcgccatca cgggcgggaa ggtggtgatc
     4381 agcgcgcggc acgggtcgag gttcagcgca ttcgacggca tgctgagagg gcggacgctc
     4441 cacaccgtgc cggggcggct gatcgtgcag gcgtggcgtt cgatgggctg gaggaagagc
     4501 gacctcgact cgaccctgat actgcgcttc atcgacaagg gcaggagcgg acgcatcgac
     4561 ctcgtgcacg tcaacgtgcc ggaccacgac tatcgcggcg tgaaccgggg gtggaagcag
     4621 tactactggc gcccctggcg gaaatatctc gcccgtatta gataagctcc atgacccgtt
     4681 tgtccccctc cgcgcgctcc gcagcggcgg atgatgcgat cgtcacgatc gaccacatcg
     4741 tcccgcacgt ctccacggtg ccggcaaacc tcggccagac cgtcgggctg ttcgtgcggg
     4801 agaaactgcc cgccggcacc caacgcccgg cgcggccccg cgtggcgctg ttcattcacg
     4861 gcggctactc gcccgcggtg gtcgcctacg atctcgatta catgggcttc agcttcatgg
     4921 cggcactggc gcgcgcgggc ttcgatgtct tcgccatgac gcacacgggc tacggcgcct
     4981 cgcccaagcc gatgatggac gatccgtgca acgtggacgc cgccttccag ccgctcatca
     5041 tcccgcacgt tctgaaggcg ccgtgcccgc cgcgctaccc gttcaagctg gtctcgagcc
     5101 gcaccgagtg ggacgagatc gcggcggtag tcgatttcat ccgcgagctg cgcggcgtcg
     5161 agcgcgtgag cctcatcggc tggtccacgg gcgcgccgcg cgccggcggc ttcgcggcgc
     5221 tccatcccga ccaggtggac aagctcgtgc tgttcggacc gtcgcctttc ttcgcgggcg
     5281 atgacccgcc ggcgcaaatg cccgagccgg gcgcgccggt gatcctgcaa acgaaggaaa
     5341 tgctgctcga gaagcgctgg cgcaacgacg tgcgctgcga cggccaggtg gacgacccgg
     5401 aggtgcgcga agtgatgtgg cgcgagctga tccggcagga cgggctcggc gcgcactggc
     5461 ggcccgaggg cgccatgcgc gcacccaacc gcatgaacta cggctggcgg cgcaattgcg
     5521 cggcgatccg ggcgccgacg ctcgtgctcc tgggcgagtt cgacaactac cggcagcggc
     5581 gggacgcgtg gaagggcctg ggcgtggacc gcagggcgct catcaaagtg gcctgcgcct
     5641 cgcacttcat gcagtacgag cgcggccgcc acgttctcca ccgcgcgacg cgcgaatggc
     5701 tggaaaccgg ctccatcgaa ggcatgaaac acgccgaatt ccacgccgat gcggaaggca
     5761 ggctcacgaa actcagggag gcaggctgac ggatgcggga tgcaagatgc gcgctgcggg
     5821 ctgcgggcgg aaggatgtgg caatgaggca gaaggcagaa ggaggaaggt aagtgatctg
     5881 cgccagggcc tcttgatgtt gaattcgtga tttgatgtgt catttcaacc gcaatctgcc
     5941 tcagcgctcg tctgctttcc gcttctcttt gttacgtaac ataaactttt gaacttttcc
     6001 agctgcggtc cggggtattt ccccgaattc gatttccttg gggcacttga agtgcgctaa
     6061 acggtcccga caaaaggcga tgatttcctc ctcggtggga gaggttccgg gcttggggtg
     6121 aaccaggcac ttgaccacct cgccccacgt ttcgtcaggc tttccgaaac aaaccacctc
     6181 attgacatcg gggtgctggt agatgcagtt ttccacttcg acgccgatga tgttctcccc
     6241 gccgctgatg atgacgtcct tgaagcgatc cttgacttcg acatagccgt cgggatggaa
     6301 aacgccgcca tccccggaat agaaccagcc gcccctgaat gccttggcgt tttcctgggg
     6361 agccttgtgg tagccctgga ccacatcgtt tccccgcatg atgatctcgc cgatagtctt
     6421 gccgtcccgg ggcacgtcat taccgtcacc atcgacaatt ctgatatagc ctgcggtgat
     6481 gtcgggcact ccctggcgca tcttcaatcc aagccgctga tcgtcgggga gggcgtccca
     6541 ttcggggtgc cattcacaga tggtgaaggg gccataggtt tcggtcaggc cgtaggcgtg
     6601 gcggatttca ccacccttga cttccatgct gcgtagcatg ttcgctggcg gaggagcagc
     6661 ggcgacgttc atgattagct tgttggggaa tttgtcgatc ccgttctcgt ccatgtaccg
     6721 gccaagccgg tggaaaacga ttggtgcgcc ggccatgtgt gttaccccgt gtttctcgat
     6781 cagcctccat atccgatcgg gatcggtgac cggcaggcag acattggtcg cccccacgcc
     6841 cgacgtaacc caggtccaac accagccgtt gcaatggaac atcgggagcg tccacagata
     6901 caccgagtag gcattgaccg aatgttcgat gatcttggca atggcgtgga ggtaggcgct
     6961 gcggtgggag tagatgcagc ccttgggatt gcctgtggtg ccgctggtgt aatcgatggc
     7021 gatgacctcg ttttcgtcct tgagctccag cctgacgtat tcgtcgctgg ccccggcgat
     7081 gaattcttcg tagccgacgc ccggcatttc cttggtgggc gtttccccat aggtgtcgcg
     7141 accgtcgaag acgttgatat aggtttcgac tttgggcagg gcatccgggt tgacccgatc
     7201 agcgacgccg gcatcgacga acatgacccg tgcttcggag aggttgacga tagtggcgat
     7261 ctccggggcg ctgaggcgat agttgatggc gacgatggcg ccgccggtca gggggatgcc
     7321 atagtgggcc tcgagcaagg gcggaaaatt gcgggacagg aaggcgacgt tgtcgcctct
     7381 ttcaacaccg aggcccttga gggcgttgct caaccggttc acccggccaa aaaactcctt
     7441 ccaggtccaa cacctgtctt tgtcgatcac cgccatcctg tccggcatca catgggcgac
     7501 gcgctcgaga aaactcaatg gcgtcaatgg ctcgtaccac gcttttaatt gagctgtcat
     7561 tcagccatcc tcccgtcagg cagtgctccg caatccgcaa aacacgctgc aaaacacccg
     7621 acccgcaacc agcttgtcct gcggcctccg caaccgatcg cccgcagaaa gcacggctag
     7681 catgttctcc cgatctgtgt gttgacatta atcaaatagc aggcaaagga cggtcgattt
     7741 ccaagccggg gctggaacca cagatctgaa gttacagcca gcggaaatga ccccttcggg
     7801 tcgcttaccg ccatgtcgca acgcggccgg ttcatgctca agacgtcgcg aaagcgtata
     7861 tagtcatcgc acgacacgcc atcaggagga ttcgtatggc cccggttccc gcattgatct
     7921 tcgccctttg cgctttgttt gcggcggtga gtgctttcgc acagacggcg gtcgccactt
     7981 atccgaacaa acccctaaga ctggttcacg gctttcctgc cggggggccg gcggacatct
     8041 tcagccggat aatcgcccaa aaactcagcg aagtcgtggg tcagcaggtc atcgtcgaca
     8101 gccgcccggg cgccggcagc atcatcgcga ccgagcacgt cgcaagagcg cccgccgatg
     8161 gctacaccgc gtatttcgcg agcagcgcca tactcgcgct gtatcccaat ctgtacgaca
     8221 agctgccgta cgacctcaac cgcgatttcg tgccggttac gctcgcggtc gccgtgccgg
     8281 aactgctggt cgtgcatcct tcgttgccga cgaggacggc gaaggagttc gtggcgctcg
     8341 cgaaggcgaa cccgaaacag ctcatttatg gctcgaccgg caacggcaac atgccgcacc
     8401 tcgcgatgga gtcgttcaag atcgcagccg gcgtcaacat cctgcacgtg ccgtacaagg
     8461 gcgccgcgcc ggcggtcgcg gatctgctcg gcgggcacgt gcacacggtg atactcgacg
     8521 tcccggtgct gctgccgcac gttaaatcgg gcaaggcgcg cgcgctcgcg atcgcgaccg
     8581 gcaagcggtc accggccctg ccggatgttc ccacaatggc cgaagcgggc tacccgtcgg
     8641 tcaacgccga caactggtac gggatcatcg tcgccgccgc gacgccgaaa gacgtcgttt
     8701 ccaggctgca tgcttcgctg gtcgcggcgc tgcaggcgcc cgagacgagg cagcggatgg
     8761 agaatcaggg cgccaacgcc ctgtcgtcga gtccccagga gctcgcggcg cacatgcgga
     8821 acgaaacgat caagtggggc aagatcatca agaccgccgg cattaaactc gacgcgcagt
     8881 aaccttctcc cgtctcaggg aaggcattcg tcgacgccgt cacgtgcggc atcgagcggc
     8941 attcgaagtt gttcactcgc tgcgcaataa aaaagtaccg cagcggggcc gttattttga
     9001 ttggggctct tctttcagga aggaacgcag ttgctcccga aactccggcg tatccttgtg
     9061 gccgtggctt gtgatcgcgt ggcgaagcgc gacctcgaat acttcttcct cagtacccgc
     9121 gatgtaaaga gagcagttct tctcgctggg atagagcctg caatcggcga aaacgcggcg
     9181 ttttgctgcc atgacgggcc tcctttctcg gtggaggcta aacgctatac cggcgttcac
     9241 gccgatgtca aattgggggt gtcttcccgc cgctggggcc ttcctggcca atgcggcccg
     9301 aagcctggct gcatcacggc gttgccccgt aactgatcga gaaagctcga gtaactacgt
     9361 gatgaagtga cgggtccaac cctcgttcct catccccctc ctcatccctg gtcaacgacg
     9421 ctgatcttgt cgccgacgcg gacccgcccg ccggcgccgc gggtcaccat tccgatcgcg
     9481 aaggtcggct gcttctggct gaacgccttc atcagcgtcg gcatgaccgc aatgtcgcgc
     9541 tcgccggtgc gggggttggc gtgcgtggcg aggcagcgga ttttccggcg cacgacctcg
     9601 aactcgaccg ctccgatgcg cagcctgcgc cccatccagt tctgctcttc ccaggcgttc
     9661 acgccttcga tcgcgatgtt ggaacggaag cggacctcgt cgatggcggg atcgccggtg
     9721 gccgctgcga ccgccgccag gctttcccgg ctgtgcagcg tcgcgtgccc gccatcgttg
     9781 tcctggtagc gcggcgtgac gccgtccccg acgagcttga gcggcaggcg ctcggcgcgg
     9841 ccggcgaggg ggttcacgtc gagcttcagt acgtagtcgg cgagcgccgc ggccagtcgc
     9901 ctgcggccat cgacgtccag caccgcctcc gcaagcacgg cgccatcgag gctcatgcgc
     9961 agccgcagcg ccgtgtggtt gagccgcacg ttcaggcgcg ccaggccggg tgtgttcatg
    10021 agcacgacgc actgctgctt gggggcccac gcgtcgtccg gcgcgcccga atccgcgaag
    10081 cggaaggcaa gcgcccggtc gcccgcgatc cgcccttctt cgaggaccgt gagcgcttca
    10141 cactcttccg gggtgaagcc cttcaccgga taacgataga gggcgacgac gcgcggcatc
    10201 ggtatgaaat cccgcgagaa gagagcagcg ggatcggatg tcattttata atgcttttgc
    10261 aacgaagccg cgcaacgata gggtaccccg ctgccccgtc accgaccaga agtcacgaat
    10321 gaagcagcag atccggttct gcaccaccgc cgacggcgtc cggatcgcct atgcgacgac
    10381 cggcgagggg ccgcccatcg tcaaagtatc caattggctg agccacctcg agtacgaccg
    10441 ggagagcccg gtgtggcgcc actggatcgc agagctctcc cgcgaccaca cctacctgcg
    10501 ctacgacgag cgcggctgcg ggctctcgga ctgggaagtc ccggagctgt cgttcgaggc
    10561 ctgggtgcgc gatctcgaga cggtggtgga cgcggccggc atcgagcgct tcccgctgct
    10621 cggcatctcg cagggcgcct ccatcgcgat cgcctacacg gtgcgccacc ccgagcgggt
    10681 cagccacctg attctgcacg gtgcttacgc gcgcggccgg ctcaagcgca acctgacgcc
    10741 gcagcagtgc aaggaagccg agaccatggc cgagcttgcc gagctcggtt ggggcaagga
    10801 aaaccccgcg ttccgccagt tcttcacgac gcagttcatt cccgacggca cgatcgaaca
    10861 gcacaactgg ttcaacgagc tggagcgggt gtcggcttcg ccggagaacg cggcccgctt
    10921 catgcggatc ttcgaccaga tcgacgtcac cgatcttgcg ccgaaggtat cgtgcccgac
    10981 gctggtgctg cacccgaatc gtgacgcgcg ggtgccgttc gatgaaggac ggctgttcgc
    11041 gggcctcatc cccggagcgc gcttcgtccc gctcgagagc cgcaaccata tcctgctcga
    11101 gaacgagccg gcatggcgcc aatggctcga tgaggtgcgc gcgttcctgc ccgccgaatc
    11161 cgtgggcggc gcggctttct cggagctcac cccgcgcgag cgcgatctcg tggaactcat
    11221 cgcgcagggg ctcgacaacg cgcagatcgc agcgcggctc tcgctcagcg aaaagaccgt
    11281 gaagaaccac atcaccagca tcttcacgaa gctcgaggtc gagaaccgct cacgggcgat
    11341 cgtgctcgcc cgcgacgccg gtttcggcag gaaaccccgc tagcaccctt ctctaggacc
    11401 gcggtcccgg ccctttttgg cccggaaagc cgcctgtttg atccggctgg gaccccggtc
    11461 tcattacgcg ccgtgcccgc ctcgcgacaa tgcgagccgt aggagccgcg aacggcggcg
    11521 agacggcaac caggacaagg aattaccgtg aacgaaaacc ggcaacaaaa cggcgcaacc
    11581 ccgctccaga gcaacctgct gcggctctcg gagcggcgcg aaatcgcgat ctacctccgc
    11641 gatggtgccg cgtgggtcgc tgacttcaac gacggccggg gcgagctcta taccgcgggc
    11701 gcatggtgcg gcatgggtgg cagccgcatg ctggctcacg cacagcgcag gtgcgaagtc
    11761 gaaaccattt caccgttacc cgacgaagtg gtgcaacgga tcgaatctct gcatcgccgc
    11821 atggagaaag cggtcgttgg cccggcgctg cggcgagcgc ttgccattct ggtcgccgtc
    11881 ttccgccatc cggggtcctc ggttcaacct tactgagagg agataaccat gcaagctgca
    11941 caacatcaga tcgacaccaa acgctacgcc aagtgcgtcg aagtctcgaa gcgcatccgc
    12001 tgggacatag accgcgacgt gatccgcggc cgcacgttcg atttctcgag gaaattcctg
    12061 cctgacgggc tgtcccttgt ggataagctc gactttctcg acgcgaacga gcgcc
//
LOCUS       DBNGWZ010000366        24122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2941014, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000366 DBNGWZ010000000
VERSION     DBNGWZ010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 24122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24122
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2941014"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..292)
                     /locus_tag="AC16CX_10420"
     CDS             complement(8..292)
                     /locus_tag="AC16CX_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR3012961.1"
                     /translation="MTKSELIAKLAVRYPQLVAKDAELAVKMILDAMGKSLAQGQRIE
                     IRGFGSFGLNYRPPRTGRNPKSGEKVQVPAKYVPHFKAGKELRERVDIKK"
     gene            complement(302..2011)
                     /gene="rpsA"
                     /locus_tag="AC16CX_10425"
     CDS             complement(302..2011)
                     /gene="rpsA"
                     /locus_tag="AC16CX_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3012962.1"
                     /translation="MKTSTPAATTENFAALFEESLARKEMRVGEVITAEVIRADENYV
                     VVNAGLKSESYIPIEEFRNDRGEIEVKTGDFVSVAIESLEDGHGETRLSRDKAKRLAA
                     WHELEEALEKGTVVTGVVNGKVKGGLTVTVNGIRAFLPGSLVDIRPVKDTAPYENKQM
                     EFKVIKLDRKRNNVVVSRRAVLEATQGAEREALLASLQEGAVVKGVVKNITDYGAFVD
                     LGGIDGLLHITDLAWRRVKHPSEILSVGDEVEAKVLKFDQEKNRVSLGLKQLGEDPWV
                     GLSRRYPPGTRLFGKVSNLTDYGAFVEIEAGIEGLVHVSEMDWTNKNVHPSKVAQLGD
                     EVEVMVLEIDEERRRISLGMKQCMPNPWEEFAMNRKKGDKVRGQIKSITDFGIFIGLP
                     GGIDGLVHLSDLSWSKPGEEAVRDYKKGQETDAVVLSIDVERERISLGIKQIEGDPFT
                     SYIQAHEKNSLVTGTVKSIDAKGVVIQLDSDVEGYLRASEVARDRVEDIHTRLKEGDK
                     VTAMVINIDRKNRTINLSIKAKDMAEEAEAMKKLSGEKQASTGTTNLGALLKAKLDVA
                     NQQ"
     gene            complement(2088..2792)
                     /gene="cmk"
                     /locus_tag="AC16CX_10430"
     CDS             complement(2088..2792)
                     /gene="cmk"
                     /locus_tag="AC16CX_10430"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3012963.1"
                     /translation="MPASSPSPVTRHSPFVPVIAIDGPSASGKGTVAQGVASVLGFHY
                     LNSGALYRTVAVAAIKAGIDLENESRVSDMALNLNANFSRDVVELDGCDISQATQTEE
                     VGTVASRIAALPKVRSALLERQRAFRQPPGLVADGRDMGTIVFPDAVLKIFLTASPEE
                     RATRRYKQLMEKGMNANMAALLQEVRERDQRDSTRATAPLQKAADAVLIDTTRRSAAD
                     AIAQVLALYKQAPRAP"
     gene            complement(2792..4102)
                     /gene="aroA"
                     /locus_tag="AC16CX_10435"
     CDS             complement(2792..4102)
                     /gene="aroA"
                     /locus_tag="AC16CX_10435"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3012964.1"
                     /translation="MAVNYQVEPGGVLKGRIRVPGDKSISHRSIMLGSIAEGVTRVTG
                     FLEGEDSIATMNVFRALGVKIDGPRQGNVTVHGVGLHGLKAPAGALDCGNAGTAMRLL
                     AGLLAGQWFDCELTGDDSLRRRPMKRVAEPLNRMGARIETAEDGRPPLRIYGGRKLAG
                     FSYAIPVASAQVKSALLLAGLYAAGRTCVTEPAPTRDHTERMLQALGCPVDKKDGTIC
                     VEGGKALRATDIQVPADISSAAFFMVGASIAQGSDITLEHVGINPTRTGAIEILRLMG
                     ADLEIRNERTAGGEPVADIRARSARLRGIEVPPRLVPLAIDEFPALFVAAANAEGETV
                     VRGAEELRVKESDRIQVMADGLATLGVKARPTPDGMRIRGGAYRGGTVTSGGDHRVAM
                     AFAMAALRASGVVTIHDCANVATSFPGFVELSRTAGLRIDESTN"
     gene            complement(4211..5095)
                     /locus_tag="AC16CX_10440"
     CDS             complement(4211..5095)
                     /locus_tag="AC16CX_10440"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR3012965.1"
                     /translation="MSSRLRINKLVVIGVGLIGSSFALALKRARAVGHVTGVGRTRRN
                     LAAALRLKAIDEASQDLPRAVRGADLVLLATPVGQMPAVMTAIAPHLPAHAVVTDAGS
                     TKRDVIACARRFLGGHFPRFVPGHPVAGTEKSGAAAALPELFRGKNIILTPQPETAAS
                     ATRLVRRAWETCGARVMRLDAEEHDAVLAAVSHLPHVIAFALVCMLSRRRNPARLFSL
                     SAGGLRDTVRIAGSSPEMWADICAANRDALLAVLEDYENELEEMRAAIESGDAAALRQ
                     MFEQARSAREKWLVKKRD"
     gene            complement(5210..6310)
                     /gene="hisC"
                     /locus_tag="AC16CX_10445"
     CDS             complement(5210..6310)
                     /gene="hisC"
                     /locus_tag="AC16CX_10445"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3012966.1"
                     /translation="MDICDLAPGYIRAIAPYQPGKPISELAREMGIEEAGIIKLASNE
                     NPLGVSPLARRAIEAVLADLARYPDGNGFELKQALSRRDGVAQECIVLGNGSNDVLEM
                     AARAFLAPGLEAVYSQHAFAVYPLAVQAAGASGVEVPARDYGHDLAAMRRAVTPKTRL
                     AFIANPNNPTGTLARPDELEAFIAGLPREVLVVLDEAYNEYLPDGIKPDSIRWLKRFP
                     NLVVTRTFSKVYGLAGLRVGYAYAAAGVADLMNRVRQPFNVNSAALVAATAALEDVDF
                     VRRSYELNRSGMRQVTDGFKRLGIEYIPSYGNFVSFKIRNAAGVFQKLLKAGVIVRPI
                     ASYGMPEHLRVTIGLESENARFLAALERAVAP"
     gene            complement(6411..7475)
                     /gene="pheA"
                     /locus_tag="AC16CX_10450"
     CDS             complement(6411..7475)
                     /gene="pheA"
                     /locus_tag="AC16CX_10450"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3012967.1"
                     /translation="MADKLKTIRDRIDALDKRLVKLLSARARLAQRIGRLKENAAYRP
                     EREAQVLRGILAENRGPLADAALARLFTEIMSACRALEDSMTVAYLGPPGTFSQEAAI
                     KHFGGITALAPCPSIDEVFRQVETGAAGYGVVPVENSTEGAIGRTLDLLLSTPAKICG
                     EVMLPIRQCLMSRSGKLAGVRRIYSHTQSLAQCQQWLARNLPRAERTAVVSNAEAARL
                     AARVRTAAAVGPRTAAALYGLKLIARDIEDEAKNTTRFLVIAERDATPSGRDKTSLIM
                     SARNVPGAMHDLLTPLAVNQVSMTRLESRPSRTGLWEYVFYVDVEGHQQDPNVARALA
                     ELKQKASFLKNLGSYPAAVA"
     gene            complement(7595..8785)
                     /locus_tag="AC16CX_10455"
     CDS             complement(7595..8785)
                     /locus_tag="AC16CX_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3012968.1"
                     /translation="MADSAYKILTLNSISSVGLKRFPSGRYVVGGQVESPDAILVRSH
                     DMHAMDIPARVRAIGRAGAGVNNIPVDRMSERGVPVFNAPGANANAVKELVIAGLLIA
                     TRNLVPALHFVEGLSGDDKTLHKLVEDGKKQFAGTELPRHTLGVIGLGKIGSLVADAA
                     IKLGMSVLGYDPEITVDAAWSLPSQVKKAHSIEEVLKAADFVTVHVPLLDVTRGMINA
                     GRIKLMKHGSILLNFAREGIVDNDAVLDALAAKRLRYYVCDFPAKKLAGHPHVITLPH
                     LGASTREAEDNCAVMVVDQVRDFLEHGNVLNAVNFPDVVMGREAPYRVGIANANVPNM
                     VGQISTAMAKAGLNIHNMINKSKGEMAYTLVDVDSKVPAKVIEQVGKIEGVLSVRYLP
                     AENP"
     gene            complement(8778..9860)
                     /gene="serC"
                     /locus_tag="AC16CX_10460"
     CDS             complement(8778..9860)
                     /gene="serC"
                     /locus_tag="AC16CX_10460"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HIR3012969.1"
                     /translation="MARVFNFGAGPAMLPEPVLRQAAEEMLDWRSSGMSVMEMSHRGK
                     EFIAIHAQAEADLRELLAIPKNYKVLFLQGGAAAQFALVPMNLLRAKGLADYVATGYW
                     SKKAIAEAKRYCEVNVAASSEDGKFTYVPKQDKWKLDPQAAYVHIASNETIEGVEWHW
                     IPDTGDVPLVADASSHILSRPLDVGRFGLIYAGAQKNIGQAGLTIVIVRDDLIGRAAP
                     DTPAVFDYKVQADADSMHNTPPTFAVYIAGLVFQWLKKLGGLRKMEEINIAKAKLIYD
                     YLDQTEFYHSPVAKEDRSRMNVQFRLRNEKLDAEFLKQAESSGLTQLKGHRALGGMRA
                     SIYNAMPIEGVQALVEFMRGFEKAHG"
     gene            complement(9953..12499)
                     /gene="gyrA"
                     /locus_tag="AC16CX_10465"
     CDS             complement(9953..12499)
                     /gene="gyrA"
                     /locus_tag="AC16CX_10465"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3012970.1"
                     /translation="MDQIAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRILYAMYEANNVWNRPYVKCARVVGDVLGKYHPHGDAATYEALVRMAQDFSMRYTL
                     IDGQGNFGSVDGDSPAAYRYTECRLERITSEMLADIDMETVDFVPNYDGKEQEPVTLP
                     TRIPNLLVNGSSGIAVGMATNIPPHNLSEVVDACQTLLKDPDVGIDDLIKIVPAPDFP
                     TRGIIYGVQGVKEGYRTGRGKVVMRARTHFEEAEKGGRTYIVIDELPYQVNKANLLMK
                     IGEMVREKKLEGIADIRDESDKTGMRAVIELKRGENADVILNNLWKETQLEETFGMNM
                     VALVDGQPRLLNLKQFLDAFLRHRREVVTRRTVFELRKARERGHILEGLAVALSNVDD
                     VIALIKAAATPADAKNELMARFWRSKLVEELLARSEGQGSRPEGLSADFGLQKGGYKL
                     SDVQAQRILEMRLQNLTALEQDKITSEYKETMDKIVDLIDVLAKPARVTQIISQELTD
                     VESHYGDERKSEIVTMTQELSTEDLIAAEDVVVTLSHGGYMKSQPVADYRAQKRGGRG
                     RQATATKEDDFVEKLFIANTHDYILCFSNRGRVYWLMVYEVPQGGRASRGKPIVNLVP
                     LMEHEKITAVLPVSAFDDEHFVFMATAMGTVKKTALSAFSRPMSKGIIAVSLDDGDHL
                     IGVALTDGKQDVMLFSNAGKAVRFAEDEVRAMGRSATGVRGMKLQKDQQVISLLTASD
                     EKLSVLTATENGYGKRTLLAEYTRHGRGTQGIIAIECSERNGKLIGAQLVGTEDEMML
                     ITTGGVLIRTRVSEVREQGRSTQGVRLINLDEGEKLAGLEKIVETNGEEE"
     gene            13031..13801
                     /locus_tag="AC16CX_10470"
     CDS             13031..13801
                     /locus_tag="AC16CX_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3012971.1"
                     /translation="MNSAIRMTLVGLAAAGVFLPAPASAQFKNQGYLTDSTGAVVMAP
                     GSGVCVRTSDWTPARAIAECDPDLVKAAPKAAPPKAAPAPKEKPKPEAKKAKPKILNI
                     EEKIELQGMPFGKAEMTADNKKELDNFLDGLRKATKARSAVRFGAVVVTGHTDRIGSL
                     KYNMKLSERRAVVVKDYLVSTGVDQKVIFWEGKGPKQPIPVTKFCDNKMKRKQLIECL
                     SPNRRVTVEVVGSAESLAKPKPKAEAKPKAKPKAKPSR"
     gene            13913..15181
                     /locus_tag="AC16CX_10475"
     CDS             13913..15181
                     /locus_tag="AC16CX_10475"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HIR3012972.1"
                     /translation="MDHSKAVLNDHAVVVANGRIRELLPARQARERYPAARRTELPGH
                     VLIPGLINLHTHAAMTLMRGLADDRALMDWLQNHIWPVEARLVSEAFVRDGTLLACAE
                     MLRGGITCFNDMYFYPEAAAHAALSAGMRAALGIIVIEMPSAYAADAQDYLSKGLAAR
                     DGLKNENLLSFCIAPHAPYTVSNKTFERIAMLQGELELPLHIHLHETRDELEQSLARY
                     GVRPLARLQDLGLVDASLIAVHAVHLTPQEGILLAEKGCHIAHCPSSNLKLASGFEPP
                     SRLLGNGVNVGLGTDSAASNNRLDLFMEMRLAALIAKGASGDPTLLPAHVVLEMATIR
                     AARALGIEDRVGSLVPEKSADMTAVNLTSLELSPCYDPVSHLVYAAGREHVSHVWVNG
                     ELLVENGRLTRLDTRALAAKAAYWKEKIQG"
     gene            15261..15956
                     /gene="ubiG"
                     /locus_tag="AC16CX_10480"
     CDS             15261..15956
                     /gene="ubiG"
                     /locus_tag="AC16CX_10480"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR3012973.1"
                     /translation="MINVDPLEIEKFSQLAHRWWDPGAEFKPLHDINPLRLDYIDGIA
                     GLRGKNVLDVGCGGGILAESMAARGARVTGIDLADKPLKVAQLHLLESGLPVDYRLIA
                     PETLAREMPASFEIVTCMELVEHVPDPAALVSACADLVQPDGRAFFSTINRNLKSYLL
                     AVIGAEYVLRLLPRGTHDYARFIKPSELAAMCRAAGLQVTGMTGMTYNPFTKVYALGP
                     DADVNYILHTIKA"
     gene            16039..16686
                     /gene="gph"
                     /locus_tag="AC16CX_10485"
     CDS             16039..16686
                     /gene="gph"
                     /locus_tag="AC16CX_10485"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050243.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HIR3012974.1"
                     /translation="MIKVVLFDLDGTFADTAPDLAYAINRMRETRGLPPLPPSATRPV
                     ASLGARGLLNAGFGIGPDNPHYRAMREEFLADYERNICRDTRLFPGIAELLEALEARG
                     LRWGIVTNKAEHLARLLLGMLQVAPRAACVIGGDSTPHIKPHPDPLLAACSAIGESPG
                     ACIYVGDDQRDIEAGRAAGMKVAAVNWGYLNGGDPESWNADWMLDTPQDLLKYLP"
     gene            16828..17190
                     /gene="ssrA"
                     /locus_tag="AC16CX_10490"
     tmRNA           16828..17190
                     /gene="ssrA"
                     /locus_tag="AC16CX_10490"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(17282..17485)
                     /locus_tag="AC16CX_10495"
     CDS             complement(17282..17485)
                     /locus_tag="AC16CX_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3012975.1"
                     /translation="MATGTVKWFNDAKGFGFITPDDGGEDVFAHFSEIQGNGHKSLKE
                     KQRVSYEVARGPKGLKAASIKPL"
     gene            complement(18029..18586)
                     /locus_tag="AC16CX_10500"
     CDS             complement(18029..18586)
                     /locus_tag="AC16CX_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012783.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR3012976.1"
                     /translation="MTDLAGKKVAYYSYAGIIDSSGVTRVAAALNAAVNNGYDEVYLC
                     LSSLGGYVGDGVYLYNHIRGLPIKITTHNTGSISSVAVAVFVGAAERLCSPHGIFMIH
                     PTTIGPFQEAVPWERLDAALKAALAEDQRTENILRERTGLPNEMLSARRVREVHITPD
                     EALKWGLVQGVREFSLPKGNEIIQI"
     gene            complement(18597..19427)
                     /locus_tag="AC16CX_10505"
     CDS             complement(18597..19427)
                     /locus_tag="AC16CX_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040609789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HIR3012977.1"
                     /translation="MNRLSLQDRARILGCLTEGNSLRATVRMIGCSINTVTKLLVDVG
                     TACAIYQNEILRNLTVRRVQVDEIWSFVGMKQKNVAPNRQGVFGQGDVYTWVALDADT
                     KLVPSWLVGTRDAEYAQVFINDLASRIKGRTQLTSDGHKAYLSAVEDAFGSEVDYSML
                     VKLYGAAEGNQQERRYSSGKCCGTITGTVCGDPDPAHVSTSFVERQNLTMRMSMRRFT
                     RLTNGFSKKVENHEHAVAMHYMHYNFGRIHKTLRVTPAMQAGVSDHVWTLEEIAKLAN
                     "
     gene            19564..19962
                     /locus_tag="AC16CX_10510"
     CDS             19564..19962
                     /locus_tag="AC16CX_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3012978.1"
                     /translation="MDYVSEAGTNQVKRWSKKTLTDQERSDMETLLSVLAKQKQWQMP
                     DFKTLSNMQGLGEIRWRSPQRTPLRLIGMRGEGAQYILLIGCSHKGTVYTPADALETA
                     LKRKRSLANGTGSICEHEEDDGTTEEEQAQ"
     gene            19931..20413
                     /locus_tag="AC16CX_10515"
     CDS             19931..20413
                     /locus_tag="AC16CX_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3012979.1"
                     /translation="MERLKRSKPNRAAFVASQINIGIPFQIRALRKQREWDQKKLAHE
                     SGMLQPRISAMEKPGYGSLTLETLKRLAAAFDVALVVRFAPFSELVRWHDKFSPDEFA
                     IPDFDEELSSVSESSEGNVIAFNYLSIVDAKVTSLPGAKAEANVATTEGTSVAFKQVA
                     "
     gene            20432..20800
                     /locus_tag="AC16CX_10520"
     CDS             20432..20800
                     /locus_tag="AC16CX_10520"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR3012980.1"
                     /translation="MADIKEQIAKLAAAIPLAKGQDHRVTYANSSRMGISPWDVRIIF
                     GQVIEAANGTQVNEDQTTVVMAPALAKQVLKNLESTVAQYEELFGEIKDPMAKSGLAQ
                     GAQQPQQQKTRKPPKLKKLS"
     gene            complement(21140..21751)
                     /locus_tag="AC16CX_10525"
     CDS             complement(21140..21751)
                     /locus_tag="AC16CX_10525"
                     /inference="COORDINATES: protein motif:HMM:NF022337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HIR3012981.1"
                     /translation="MAWFLFLLAVAFIAAAVVWNYRKKAALRESVSKERFERIFKTKA
                     STAPASESNPAGVPDTGVGSMPTAPVAAATRPAGERFLGRSETLIYFLLKTGLPDHEV
                     FANVSLASVVGAPGGESEREQQVRRLSQYRLDFVVCDRDMRITAVVEVEAPGGADAAG
                     TQRFKSDCLKTAGIRLVRINPASPPRRKEIRALICGGPGAAGT"
     gene            complement(21762..22994)
                     /locus_tag="AC16CX_10530"
     CDS             complement(21762..22994)
                     /locus_tag="AC16CX_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3012982.1"
                     /translation="MGSVAKAYNIADLREMALKRVPKGLFEFVDRGTEDEVSLRHNRV
                     IFERIRFKPRTLVDVSKRTQEITLFGKRHKMPIVIAPTGTAGLMWYQGEIGLARAAAE
                     AGIPFTLATGSMTAMETVAEQAGGTLWFQLYMWPDRSLSHKIVERAKAAGYEALVVTV
                     DGAVSGNREYNLRNGFTIPFKFTHRNVTDVMLHPRWLLQVLARYVLTTGMPRYENYPS
                     EIKNRITARPMGRSMMKCDSLNWEDLKALRRMWPHKLMVKGILHPQDAVLAADNGADG
                     VIVSNHGGRNLDGAISPLEALPDIVDAVGKRITVLVDSGFRRGSDVVKALAMGAHAVQ
                     IGRSTLYGVAAGGEEGAKRAVTIFREEIDRVMALIGCRSIAELSPEFLLAPDFPLRAA
                     DPVRPGLKLLDTAGSRTA"
     gene            complement(23088..23387)
                     /locus_tag="AC16CX_10535"
     CDS             complement(23088..23387)
                     /locus_tag="AC16CX_10535"
                     /inference="COORDINATES: protein motif:HMM:NF025363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HIR3012983.1"
                     /translation="MNRTEDRLIEIARRKERLIARAEAQRAAIGESIRRLEGPIGIVD
                     RGLEVARFLQAHPVLVGAAVAALVVFSRRGLPSLAGRVFSAWRLWRWVSAWAMPR"
     gene            complement(23384..23776)
                     /locus_tag="AC16CX_10540"
     CDS             complement(23384..23776)
                     /locus_tag="AC16CX_10540"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3012984.1"
                     /translation="MPEPALGEAGAGIVQSLRKLAATLVALLRTRLELLATELEEERI
                     RFLQLLFWAAAALFFFAISVLLLTVLIVVVFWDSYRTASIAVLAGIFLAAGIGTAIGV
                     RNRLNARPRLFSASLDELAKDKDQLISR"
     gene            complement(23790..24101)
                     /locus_tag="AC16CX_10545"
     CDS             complement(23790..24101)
                     /locus_tag="AC16CX_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3012985.1"
                     /translation="MTPDSAVTKEKLVQDLKIVISDAEELLRATASQAGEKVSAAREK
                     IQESLNRAKVKLADVEDVIIDKSKQAARATDEYVHDHPWRAVGIAAGIGFIIGLLISR
                     R"
BASE COUNT         4378 a         7754 c         7287 g         4703 t
ORIGIN      
        1 ggaatgctta tttcttgata tccactcgtt cgcgcaattc cttcccggcc ttgaagtgcg
       61 gcacgtattt ggccggcacc tgtaccttct cccccgattt tgggttgcgc ccggtgcgcg
      121 gagggcgata attgaggccg aaactgccga aaccccggat ctcgatgcgc tgaccctgcg
      181 ccaggctctt ccccatggcg tcgaggatca tcttcaccgc gagttccgca tccttcgcca
      241 ccaactgcgg gtagcgcacc gccagcttcg cgatcaactc ggatttggtc atggtgctcc
      301 cttattgttg attggctacg tccagcttgg ccttgagcag cgcgccgagg ttggtggtgc
      361 cggtgctcgc ctgcttttcc ccggagagct tcttcatggc ctcggcctcc tccgccatgt
      421 ccttggcctt gatcgataga ttgatggtcc ggttcttgcg gtcgatattg atgaccatcg
      481 ccgtcacctt gtcgccttcc ttgagccgcg tgtggatgtc ctccacgcgg tcgcgcgcca
      541 cttccgaggc ccgcagatag ccctcgacgt cggagtcgag ctggatgacg acgcctttgg
      601 cgtcgatgga tttcacggtt ccggtgacca gcgaattctt ctcgtgcgcc tggatgtagc
      661 tcgtgaaggg gtcgccctcg atctgcttga tcccgagcga gatgcgctcg cgctcgacgt
      721 cgatcgaaag caccacggcg tcggtctcct ggcccttctt gtaatcgcgc acggcctcct
      781 cgccgggctt gctccacgag aggtctgaca ggtgcacgag gccgtcgatg ccgccgggca
      841 gcccgatgaa gatcccgaaa tcggtgatgg acttgatctg cccgcgcacc ttgtcgccct
      901 tcttgcggtt catggcgaat tcttcccacg ggttgggcat gcactgcttc atgcccagcg
      961 agatgcggcg gcgctcctcg tcgatctcga gcaccatgac ctcgacttcg tcgccgagct
     1021 gcgccacctt ggaaggatgc acgttcttgt tggtccagtc catttcggag acgtgtacca
     1081 gcccctcgat cccggcctcg atctcgacga acgcgccgta gtcggtgagg ttggagacct
     1141 tgccgaacag ccgcgtgccg ggcggatagc ggcgggacag cccgacccag gggtcctcgc
     1201 cgagctgctt gagcccgagc gaaacgcggt tcttctcctg gtcgaacttg agcaccttgg
     1261 cttcgacctc gtcgcccacc gagaggattt cggagggatg cttgacgcgg cgccaggcga
     1321 ggtcggtgat gtgcaacagc ccgtcgatgc cgccgaggtc cacgaacgcg ccgtagtcgg
     1381 tgatgttctt gacgacgcct ttcaccaccg cgccttcctg caggctcgcg agcagcgctt
     1441 cgcgttcggc gccctgggta gcctcgagca cggcgcggcg cgacaccacc acgttgttgc
     1501 gcttgcggtc gagcttgatg accttgaatt ccatctgctt gttttcatac ggcgcggtgt
     1561 ccttgaccgg acggatgtcc acgagcgagc ccgggaggaa ggcgcggatg ccgttcaccg
     1621 tgacggtgag cccgcccttg accttgccgt tgaccacgcc cgtcacgacc gtgcccttct
     1681 ccagcgcttc ctcgagctcg tgccacgccg ccagccgctt cgccttgtcg cgcgaaagcc
     1741 gcgtttctcc gtgaccgtcc tccagggatt cgatcgcgac gctgacgaag tcgcccgtct
     1801 tgacctcgat ttcgccgcgg tcgttgcgga attcctctat cgggatgtaa ctctcggatt
     1861 taaggccggc attgacgacg acgtagttct cgtcagcgcg gatgacctcg gcggtgatca
     1921 cttcgcccac ccgcatttcc ttgcgggcca gactttcctc gaacagcgcg gcgaagtttt
     1981 ccgtggtggc tgccggggtg gatgttttca tcattcagat tcgggacgtt ggggttgacc
     2041 gtcgtggcga cggggttggt tgatcaaacc atgaaccggg gtgcggctca cggcgcccgt
     2101 ggtgcctgtt tatagagcgc cagcacctgc gctatcgcat ccgccgccga cctgcgcgtg
     2161 gtatcgatca ggacggcgtc tgcggccttc tgcagggggg ccgtggcgcg cgtgctgtcg
     2221 cgttgatcgc gctcccgaac ctcctgtaaa agggccgcca tattagcatt cattcctttt
     2281 tccatcaact gtttatatcg ccgtgtcgcc cgttcttccg ggctcgcggt gaggaagatt
     2341 ttgagcaccg cgtcggggaa aacgatggtg cccatgtcgc gcccgtccgc caccagtccg
     2401 ggcggctgcc ggaacgcgcg ctggcgctcg agcagcgcgc ttctgacttt gggcagcgcg
     2461 gcgatgcggg atgcaacggt accgacctcc tccgtttgcg ttgcctggct gatatcacaa
     2521 ccatcgagct cgacaacatc tcttgaaaag ttggcattta aattcagtgc catatccgat
     2581 actcttgatt cgttttctag gtctatacca gccttgattg cggccacggc tacggtccgg
     2641 tacagcgcac cgctgttcag gtaatgaaag cccagcacgc tcgccacacc ttgagccacc
     2701 gtgcccttgc cggacgccga tggcccgtct atggcgatca cgggtacgaa tggcgagtga
     2761 cgggtgacgg gtgacgggct ggaagccggc atcagtttgt cgactcgtcg atccgcagcc
     2821 ccgccgtgcg ggagagctcg acgaagcccg ggaaggacgt tgccacgttg gcacaatcgt
     2881 gaatcgttac cacgcctgac gcgcgcagcg ccgccatcgc aaacgccatc gccacgcgat
     2941 gatcgccgcc ggaagtcacc gttccaccgc ggtacgcccc gccccggata cgcatgccgt
     3001 ccggtgtcgg tcgcgccttc acgccgagcg tggcaaggcc gtccgccatc acctggatgc
     3061 gatcggactc cttgacgcgc agttcttcgg cgccgcgcac caccgtctcg ccctcggcat
     3121 tggccgcggc gacgaacaag gcgggaaatt catctattgc gagcggaacg aggcgcggcg
     3181 gcacctcgat gccgcgcaaa cgcgcgcttc gcgcgcggat atccgccacc ggctcgccgc
     3241 cggcggtgcg ctcgttcctg atctcgagat cggcgcccat caaccgcagg atttcgatgg
     3301 cgccggtgcg cgtcgggttg atcccgacat gctcaagcgt gatgtccgat ccctgtgcga
     3361 tcgaggcgcc caccatgaaa aaggcggccg atgaaatatc cgcgggcacc tggatgtccg
     3421 tcgcccggag cgctttcccg ccctcgacac agatggtgcc gtctttttta tccacgggac
     3481 agcccagtgc ctgcaacatg cgctcggtgt gatcgcgcgt cggagccggc tcggtgacac
     3541 aggtgcggcc cgccgcgtac agccccgcca gcagcaacgc cgatttcacc tgcgcgcttg
     3601 cgaccggtat ggcgtagctg aagccggcga gcttgcgccc gccgtaaatc ctgagcggcg
     3661 gacggccgtc ttccgcggtc tcgatgcgcg cgcccatgcg gttcaacggc tcggcgacgc
     3721 gcttcatcgg ccgccgccgc aggctgtcgt caccggtcag ctcgcaatcg aaccactggc
     3781 cggccagcaa cccggcgagc agcctcatgg cggttccggc attgccgcag tcgagcgctc
     3841 cggcgggcgc cttgagtccg tgcagcccga cgccgtgcac ggtgacgtta ccctgccggg
     3901 gaccgtcgat cttcactccc agggcgcgaa aaacgttcat ggtcgcaatc gagtcctcgc
     3961 cctccaggaa ccccgtgacg cgcgtgaccc cctcggcgat ggagcccagc atgatgctgc
     4021 ggtgggaaat ggacttgtca cccgggacgc ggatgcggcc cttcagtact ccaccgggtt
     4081 ccacctgata attgacagcc attgccctta cgccgcgtcc gatgactagg attgaggatt
     4141 gaggattgag gattgagcaa ccctagccaa actcattgga ggcattaact cgatcctcaa
     4201 tcctatcggc tcaatcccgc tttttgacca gccatttttc gcgcgcgctg cgcgcctgct
     4261 cgaacatctg cctgagggcg gccgcgtcgc cgctctcgat ggcggcgcgc atctcctcga
     4321 gctcgttctc gtaatcctcc agcacggcga gcagcgcgtc gcggttcgcg gcacagatat
     4381 ccgcccacat ttccggcgag ctgcctgcga tccggacggt atcgcgcaag cctcccgcgg
     4441 acaggctgaa aagccgcgcg gggttgcgac gtcgtgacag catgcacacc agtgcaaacg
     4501 cgatgacatg cggcagatgg ctcacggcgg ccaggaccgc gtcgtgttcc tccgcatcga
     4561 gccgcatcac gcgcgcgccg caggtttccc acgcccgccg caccagccgc gttgccgacg
     4621 cggcggtttc cggttgcggc gtcaggatga tgtttttgcc gcgaaacagc tccgggagag
     4681 ccgccgccgc gccgcttttc tcggtaccgg caaccggatg cccgggcacg aagcggggaa
     4741 agtgcccgcc gaggaaacgg cgggcgcagg cgatcacgtc gcgcttggtg ctgccggcgt
     4801 ccgtaaccac cgcatgcgcg ggcagatgcg gcgcgatggc cgtcatcacc gccggcatct
     4861 ggccgaccgg cgtggcgagc agcaccaggt ccgcgccgcg caccgcgcgc gggaggtcct
     4921 gcgacgcttc gtcaatcgcc ttgagcctga gcgcagcggc aagattgcgg cgagtgcggc
     4981 ccactcccgt cacgtgcccc accgcgcgcg cgcgcttcag agccagcgcg aacgagctgc
     5041 cgatgaggcc cacgccgatc acgacgagct tgttgatgcg caatctcgaa ctcatacacc
     5101 cgatttcacc acggagacac ggacggcatt ctgaaatcta ccacttattg attcaaggta
     5161 aagatttctt gccgcccccg gccgtcgcga tacctgcgcg gcaagtcatt caaggtgcaa
     5221 ctgctcgttc gagggcagca agaaatcttg cgttctccga ttccaggccg atggtgacgc
     5281 gcaggtgttc gggcatgccg tagctcgcaa tcggacgcac gatcacgccc gctttcagca
     5341 gcttctggaa cacgccggcc gcattcctga tcttgaagct cacgaagttg ccgtaggacg
     5401 gaatgtactc gatgccgagc cgcttgaaac cgtccgtgac ctggcgcatg cccgaccggt
     5461 tgagttcgta gctcctgcgc acgaaatcaa cgtcttcgag cgccgcggtt gccgcaacca
     5521 gtgcggcgct gttgacgttg aacggctggc gcacccggtt catgagatcc gcgacgccgg
     5581 ccgcggcata agcatagccc acgcgcagcc ccgccaggcc gtacaccttg gagaacgtgc
     5641 gcgtcaccac gaggttcggg aaacgcttca gccaccggat gctgtcgggc ttgatgccgt
     5701 ccggcaggta ttcgttgtag gcctcgtcca gcaccaccag cacctcgcgc ggcagcccgg
     5761 cgatgaaggc ttcgagctcg tccgggcgcg ccagggtgcc ggtcgggttg ttggggttgg
     5821 caatgaacgc gaggcgggtt ttcggcgtga ccgcgcgccg cattgccgca aggtcatggc
     5881 cgtagtctct cgccggcacc tcgacacccg acgcaccggc cgcctgcacc gccaggggat
     5941 agacggcgaa tgcatgctgc gaatagaccg cctccaggcc gggcgcgagg aacgcgcgcg
     6001 ccgccatctc cagcacgtcg ttcgagccgt tgccgagtac gatgcattcc tgcgccacgc
     6061 cgtcgcggcg tgagagcgcc tgcttgagct cgaagccatt gccgtcgggg tagcgcgcga
     6121 ggtccgcgag cacggcctcg atcgcgcgcc gcgcgagcgg gctcacgccg agggggttct
     6181 cgttcgaggc gagtttgatg atgcccgctt cctcgattcc catctcgcgc gcgagctccg
     6241 agatcggttt gccgggctgg tacggcgcga tcgcgcggat atagccggga gcgaggtcgc
     6301 agatgtccat tttggtgagg ggtgactcgt tactcggtac tcgttgctcg tttttcatta
     6361 cccgaccttc gctcgcgaga aacgaggaac gagaaacgag tcactttttt tcaggccacc
     6421 gccgcaggat aggagccgag gttcttgagg aacgacgctt tctgcttcag ctccgcgagg
     6481 gcacgcgcca cgttggggtc ctgctggtgg ccctcgacgt ccacgtagaa cacgtattcc
     6541 cacaggccgg tacgcgaggg ccgggattcc agccgggtca tgctcacctg gttcaccgcc
     6601 agcggcgtca acagatcgtg catcgcgccc ggcacgttgc gcgcggacat gatgagcgag
     6661 gtcttgtccc tgcccgacgg cgtcgcgtcg cgctcggcga tgaccaggaa gcgtgtggtg
     6721 ttcttcgctt cgtcctcgat atcgcgcgcg atcagcttca gcccgtaaag cgccgcggcg
     6781 gtcctgggcc cgaccgccgc cgcggtccgc acgcgcgccg cgagccgtgc cgcctccgcg
     6841 ttgctgacga ccgcggtccg ctcggcgcgc ggcagattgc gcgcgagcca ctgctggcac
     6901 tgggcgaggc tctgggtatg cgagtaaatc ctgcggacgc cggcaagctt tccgctcctg
     6961 ctcatcaggc actggcggat tggcagcatc acttcaccgc agatcttcgc aggggtcgaa
     7021 agcagcaggt caagcgtgcg gccgatcgcg ccctcggtcg agttctcgac cggaaccacg
     7081 ccgtagccgg cggcgccggt ttcgacctgg cggaatactt cgtcgatcga cgggcacggc
     7141 gcgagggcgg taatgccccc gaaatgcttg atcgccgcct cctggctgaa ggtacccggc
     7201 ggaccaaggt aggcgaccgt catcgagtcc tcgagcgcgc ggcacgccga catgatctcc
     7261 gtgaacaggc gggcgagcgc ggcgtccgcc agggggccgc ggttctcggc gaggataccg
     7321 cgcagaacct gcgcctcgcg ctcgggacga taggccgcgt tttccttgag cctgccgatg
     7381 cgctgcgcga gccgcgcgcg cgccgagagc agcttcacca gccgcttgtc gagcgcgtcg
     7441 atccggtcgc ggattgtctt gagcttgtcg gccatctatt ggtgactgag tgactaggta
     7501 attaagtgac tgagtgaatg agccacaagc ttagcagttg cgcgctctga gcgaccacaa
     7561 gcactctcag tcacccagtc atctaatcac ctagtcacgg attctccgcc gggaggtaac
     7621 gcaccgacag cacgccctcg atcttgccga cctgctcgat caccttggcg ggcaccttgc
     7681 tgtcgacatc caccagggta taggccattt cgcccttcga cttgttgatc atgttgtgga
     7741 tgttcaatcc agccttcgcc atcgccgtgg aaatctgccc caccatgttc ggcacgttgg
     7801 cgttggcgat gcccacgcga tacggcgcct cgcgccccat caccacgtcc ggaaaattga
     7861 ccgcgttcag gacgttgccg tgctcgagga agtcccgcac ctggtccacg accatcaccg
     7921 cgcagttgtc ttccgcctcg cgggtggatg cgccgaggtg cggcagcgtg atcacgtgcg
     7981 gatgcccggc cagcttcttc gccgggaaat cgcagacgta ataacgcaag cgcttcgccg
     8041 cgagcgcgtc cagcaccgcg tcgttatcca cgatgccctc gcgcgcgaag ttgagcagga
     8101 tcgagccgtg cttcatgagc ttgatgcggc cggcgttgat catgccgcgg gtcacgtcga
     8161 gcagcggcac gtgcaccgtg acgaagtcgg ccgccttcag cacctcctcg atgctgtgcg
     8221 ccttcttcac ctgcgaaggc aggctccacg ccgcatccac ggtgatctcg ggatcgtaac
     8281 cgagcacgct catgccgagc ttgatcgccg catccgcgac caggcttccg atcttgccca
     8341 gcccgatcac gcccagcgta tgccgcggaa gctcggtccc ggcgaactgc ttcttgccgt
     8401 cttcgaccag cttgtgcagc gttttgtcgt cgccgctgag tccctccacg aagtgcagcg
     8461 ccggtaccag gttgcgcgtg gcgatcaaca accccgcgat caccagttcc ttcacggcat
     8521 tggcgttcgc gcccggcgcg ttgaacaccg gaacgccccg ctcgctcatc ctgtccaccg
     8581 ggatgttgtt gacgcccgcg ccagcgcgcc cgatcgccct cacgcgggcc gggatgtcca
     8641 tggcgtgcat gtcgtgcgag cgcaccagga tggcatcggg gctctcgacc tggccgccca
     8701 cgacgtagcg gccggatgga aagcgcttca ggccgacgct ggagatggaa ttgagcgtaa
     8761 gtatcttgta agcggaatca gccatgtgcc ttttcgaacc cccgcatgaa ctcgaccagc
     8821 gcctgcacgc cctcgatcgg catcgcgttg tagatcgagg cgcgcatgcc gccgagggcg
     8881 cgatggccct tgagctgcgt caggccgctg ctctcggctt gcttgagaaa ctcggcatcg
     8941 agcttctcgt tgcgcaaccg gaactggacg ttcatgcgcg agcggtcctc tttcgccacc
     9001 ggagagtggt agaactcggt ctggtcgaga taatcgtaga tgagcttcgc cttggcgatg
     9061 ttgatctcct ccattttgcg gagcccgccg agttttttga gccactggaa caccaggccg
     9121 gcgatgtata ccgcgaaggt cggcggcgta ttgtgcatcg agtccgcatc ggcctgcacc
     9181 ttgtaatcga acaccgccgg cgtgtccggc gcggcccgcc cgatcaggtc atcgcgcacg
     9241 atgacgatgg tgagccccgc ctgcccgatg ttcttctggg cgccagcata gatcaggccg
     9301 aagcggccga cgtcgagcgg gcgcgacagg atgtgcgaag aagcatccgc caccagcggc
     9361 acgtcgccgg tatccgggat ccagtgccat tcgacgcctt cgatggtctc attggaagcg
     9421 atgtggacgt acgccgcctg cggatcaagc ttccacttgt cctgtttcgg aacataggtg
     9481 aacttgccgt cctctgagct cgcggcaacg ttcacctcgc agtagcgttt ggcttccgcg
     9541 atcgcctttt tcgaccagta gccggtcgcg acgtagtcgg cgagcccctt cgcccgcagc
     9601 aggttcatcg gaacgagcgc gaactgcgcg gccgccccgc cttgcaggaa cagcaccttg
     9661 tagtttttcg ggatcgccag caactcgcgc aggtcggcct cggcctgggc gtggatggcg
     9721 atgaactcct tgccgcggtg gctcatctcc atcaccgaca tgccgctcga gcgccagtcg
     9781 agcatttcct ccgcggcctg ccgcagcacc ggctccggca gcatcgccgg accggccccg
     9841 aaattgaata cccttgccat gaaattcccc aaggtaagcg gtgagccgtg agctgtgagc
     9901 cgtaaactcg gcgttgagca gtttcctccg cttaccgctt actgctttcc gctcactctt
     9961 cctcgccgtt cgtctcgacg atcttctcca gcccggcgag cttctcgccc tcgtcgaggt
    10021 tgatgagccg cacgccctgc gtggagcgtc cctgctcgcg cacctcggag acgcgcgtgc
    10081 ggatcagcac gccaccggtg gtgatcagca tcatctcgtc ctcggtcccg accagttgcg
    10141 cgccgatgag cttgccgttc cgctcgctgc actcgatcgc gatgatccct tgcgtgccgc
    10201 gcccgtggcg cgtgtactcc gccagtagcg tgcgcttgcc gtagccgttc tcggtggcgg
    10261 tcagcacgga cagtttctcg tcgctcgcgg tgagcagcga tatcacctgc tggtccttct
    10321 gcaatttcat gccgcgcacg ccggtcgcgc tgcggcccat cgcccgcacc tcgtcctcgg
    10381 cgaagcgcac tgccttgccg gcgttggaga acagcatcac gtcctgcttg ccgtcggtga
    10441 gcgccacgcc gatcagatgg tcgccgtcat cgaggctcac cgcgatgatg cccttcgaca
    10501 tcgggcgcga gaacgccgac agcgcggtct tcttcacggt tcccatcgcg gtagccatga
    10561 acacgaagtg ctcgtcatcg aacgccgaga ccggcagcac cgcggtgatt ttctcgtgct
    10621 ccatcagcgg caccaggttc acgatcggct tgccgcgcga ggcgcggccg ccctgcggca
    10681 cctcgtagac catcagccag tagacgcgcc cgcggttgga gaagcacagg atgtagtcgt
    10741 gggtattggc gatgaacagc ttctccacga aatcgtcttc cttggtcgcc gtcgcctgcc
    10801 tgccgcggcc gccgcgcttt tgcgcgcggt agtccgccac cggctgcgat ttcatgtagc
    10861 cgccgtggga gagcgtcacc accacgtcct cggcggcgat caggtcctcc gtcgagagct
    10921 cctgggtcat ggtgacgatt tcgctcttgc gctcatcgcc gtaatggctc tcaacgtccg
    10981 tgagctcctg cgagatgatc tgcgtgacgc gcgcgggttt ggcgagcacg tcgatgaggt
    11041 ccacgatctt gtccatcgtc tccttgtatt cggacgtgat cttgtcctgc tccagggccg
    11101 tgaggttttg caggcgcatc tcgaggatgc gctgcgcctg gacgtccgag agcttgtaac
    11161 ctcccttctg cagcccgaaa tccgccgaaa gcccttccgg acgcgacccc tggccctcgc
    11221 tcctggcgag gagttcctcc accagcttgg aacgccagaa gcgcgccatc agctcgttct
    11281 tggcgtcggc cggcgtggcg gcggccttga tcaacgcgat gacgtcgtcc acgttggaca
    11341 gcgcgaccgc caggccctcg aggatatggc cgcgctcgcg cgccttccgc agctcgaata
    11401 cggtgcgccg cgtgactacc tcgcggcgat gccgcaggaa ggcgtccagg aactgcttga
    11461 ggttgagcag ccgcggctgg ccgtccacca gcgcgaccat gttcatgccg aaggtttcct
    11521 cgagctgcgt ctccttccac aggttattca ggatcacgtc ggcgttttcg ccgcgcttca
    11581 gctcgatcac ggcgcgcatg ccggtcttgt cggactcgtc acggatgtcg gcgatgccct
    11641 cgagcttctt ctcgcgcacc atttcgccga tcttcatgag caggttcgcc ttgttgacct
    11701 ggtacggcag ctcgtcgatt acgatgtagg tgcgaccgcc cttctccgcc tcctcgaaat
    11761 gggtgcgcgc gcgcatgacc accttgccgc ggccggtgcg gtagccctcc ttgacgccct
    11821 gaacgccgta gatgatgccg cgcgtcggga aatcaggcgc cggcacgatc ttgatcaggt
    11881 cgtcaatgcc gacatcggga tccttcagca gcgtctggca ggcgtccacg acctcggaca
    11941 ggttatgggg ggggatgttg gtcgccatcc caaccgcgat gcccgaagag ccgttcacca
    12001 gcaggttcgg gatgcgggtg ggcagcgtga ccggttcctg ctccttgccg tcgtagttcg
    12061 gcacgaaatc gaccgtttcc atgtcgatgt cggccagcat ctcggaggtg atgcgctcga
    12121 gccggcattc cgtgtagcgg taggcggcgg gggaatcgcc gtcgaccgac ccgaagttcc
    12181 cctggccgtc gatcagggtg tagcgcatgg agaaatcctg cgccatccgc accagcgctt
    12241 cgtaggtggc ggcatcgccg tgggggtgat acttcccgag cacgtcgccg accacccgcg
    12301 cgcacttgac gtagggccgg ttccacacgt tgttcgcctc gtacatcgcg tagaggatgc
    12361 ggcggtgcac cggcttcagc ccgtcgcgca cgtcgggcag cgcccgaccc acgatcacgc
    12421 tcatcgcgta atcgaggtag gaacggcgca tctcctcctc gaggcttacc gggagtgttt
    12481 ccttggcgat ctggtccatg aaaaagcggg aaaaacgaag gtttttggcc gctgcttaaa
    12541 aggcgcaatt ttaccatgtg ccgacggcat gacttcgtcc acaccctcgc catttcgcgg
    12601 cagtcggccc tgcacgaaac agggtccggc cgggcttgac gaagctaccc ggcacaccgt
    12661 tcgatgcccg tgctccaaca aaccaagtcg agcatgatgt ctcacggtta cggttgctgg
    12721 ctgtggcgcg agagcccgcc ggacaggcac ccgtgtgcgg gccaatgatc gtttccgtga
    12781 caccccggaa cagggtgtca aaggccatcc gtcaaagcag tccaattcgt gaaacacccc
    12841 cctccttgag cggggcgttt cctatacgca acaggcaaat atgccttttc cctcagaacc
    12901 gtgttatgat ttctgcataa tttgccttgc caagtgggtt gtgatattag tagcccccgg
    12961 ggagactcca caggttgacc ggcgttccgg cgggtcccta accatcgcac aaacaggagg
    13021 atcaataatc atgaattcag caatccggat gaccctcgtt ggcctcgctg ccgcaggtgt
    13081 gttcctgccc gcgcccgcgt cggctcagtt caagaaccaa ggttacctga cggacagtac
    13141 aggcgcggtt gtcatggccc ctgggtctgg tgtctgcgtg cgcaccagcg actggacgcc
    13201 ggcccgcgcg atcgccgagt gcgatccgga tctggtgaag gctgcgccga aggctgcgcc
    13261 gccgaaggcc gcgcccgcgc cgaaggaaaa gcccaagccg gaagccaaga aggccaagcc
    13321 caagatcctc aacatcgaag agaagatcga gttgcagggc atgccgttcg gcaaggccga
    13381 aatgaccgcg gataacaaga aggaactcga caacttccta gacggattgc gcaaggcgac
    13441 caaggcccgc agcgcggtgc gcttcggcgc ggtggtcgtg accggccata ccgaccgcat
    13501 cggcagcctc aagtacaaca tgaagctgtc cgagcgccgc gcggtggtgg tcaaggacta
    13561 cctggtgagc accggcgtcg atcagaaggt gatcttctgg gaaggcaagg gaccgaagca
    13621 gccgatcccg gtcaccaagt tctgcgacaa caagatgaag cgcaagcagt tgatcgaatg
    13681 cctgtcgccg aaccgccgcg tgaccgtcga agtggtcggc agtgccgagt cgctcgcaaa
    13741 gccgaagccc aaggcggaag ccaagccgaa ggccaagcca aaggcgaagc cctcgcgata
    13801 agtcgaggaa cgcagtaacg aaagccctgc ctcggcaggg cttttttttt cggaggagga
    13861 ccgcctgtct gattctgccc tgactatcgt cgaggctggc tggatcgttc caatagatca
    13921 ctcgaaggcg gtcctaaacg atcatgccgt cgtggtagcc aatggccgta ttcgcgaatt
    13981 gctgcctgcg cggcaggcgc gggagcgcta ccctgccgcg cgccggaccg aacttccggg
    14041 gcatgtgttg atccccgggc tgatcaatct gcacacccat gccgcgatga cgttgatgcg
    14101 cgggctcgcg gacgaccgcg cgctcatgga ctggctgcag aaccacatct ggccggtgga
    14161 agcgcgcctg gtctcggagg cgttcgtgcg cgacggcacg ctgctcgcct gcgccgagat
    14221 gttgcggggg gggatcacct gtttcaacga catgtatttc tatcccgaag ccgcagcgca
    14281 tgcggcactg tcggcgggaa tgcgggcggc gctcggcatc atcgtgatcg agatgccttc
    14341 ggcctacgcg gccgacgccc aggactatct gagcaaagga ctggcggcgc gagacggcct
    14401 gaagaacgaa aacctgcttt ccttctgcat cgcgccgcat gccccgtaca cggtcagcaa
    14461 caagaccttc gagcgtatcg ccatgctgca gggcgagctc gagctgccgc tgcacatcca
    14521 cttgcacgag acgcgcgacg agctcgagca aagcctcgcc cgttacggcg tacgacctct
    14581 cgcccggctg caggacctgg ggctagttga cgccagtctg atcgccgtcc acgccgttca
    14641 cctcaccccc caggagggta ttctgctcgc ggaaaaaggc tgccatatcg cccattgccc
    14701 cagttcgaac ctgaaactcg caagcggctt cgagccgccg tcgcggctgc tcgggaacgg
    14761 ggtcaacgtg ggactgggca ccgacagcgc ggccagcaat aatcgcctgg acctcttcat
    14821 ggaaatgcgg ctcgccgccc tgatcgccaa aggcgcgagc ggagatccga cgctcctgcc
    14881 cgcccacgta gtgctggaaa tggccaccat tcgcgccgcg cgcgcgctcg gtatcgagga
    14941 ccgcgtcggc tcgctcgtgc cggaaaagtc cgccgacatg acggccgtga acctgacctc
    15001 gctggagctg tcgccctgct atgacccggt gtcgcacctg gtctatgcgg cgggccgcga
    15061 gcacgtgagc catgtctggg tcaatggcga gctgctggtg gaaaacggcc ggctgacgcg
    15121 gctcgacacg cgcgcgcttg ccgccaaggc ggcgtactgg aaggagaaga tccaaggata
    15181 gttttcagcg atcaggcgtc ggccggtccc tttgcctgat aatcttcaac gggtatgctg
    15241 agaacttaac gctgaaaact atgatcaacg tcgatccgct cgagatcgag aagttcagcc
    15301 agctcgccca ccgctggtgg gaccccggcg ccgagttcaa gccgctgcac gacatcaacc
    15361 cgttgcggct ggactatatc gacggcatcg ccggcctgcg cgggaaaaac gtcctcgacg
    15421 tgggctgcgg gggcggcata ttggccgaga gcatggcggc ccgcggagca agggtgaccg
    15481 gcatagacct cgcggacaag ccgctgaagg tggcgcaact gcatctgctc gaatcgggat
    15541 tgccggtcga ctaccggctg attgcgcccg agacgctggc gcgcgagatg ccggcgagct
    15601 tcgaaatcgt cacctgcatg gagctggtcg agcacgtgcc ggacccggcg gcattggtga
    15661 gcgcctgcgc cgatctcgtc caaccggacg gccgcgcgtt cttttccacc atcaaccgca
    15721 acctgaaatc gtacctcctc gccgtgatcg gcgccgagta cgtgttgaga ctcctgccgc
    15781 gcggcaccca cgattacgcc aggttcatca agccttccga gctggccgcg atgtgccgcg
    15841 ccgccggcct gcaagtgacg ggcatgaccg gcatgaccta caaccctttc accaaggtgt
    15901 atgccttggg ccccgatgcg gatgtcaatt acattctcca caccattaaa gcgtgataag
    15961 tgataggtga taggtgatag gagccaactt ccgcgcgcgc gcgggtaact tatcacttat
    16021 cactcgtcac gcatttcgat gatcaaggtg gtcctcttcg acctcgacgg cacgttcgcg
    16081 gacacggcgc ccgacctggc gtacgcgatc aaccgcatgc gtgaaacgcg ggggttgccg
    16141 ccgctgccgc cttccgccac ccgtccggtt gcatcgctgg gcgcgcgcgg cctgctgaac
    16201 gcgggtttcg gcatcggccc ggacaatcct cactatcgcg cgatgcgcga ggaattcctg
    16261 gcggactacg aacggaacat ttgccgcgac acccgcttgt tccccggtat cgccgaattg
    16321 ctggaggcgc tcgaggcacg cgggttgcgc tggggcatcg tgaccaacaa ggcggagcac
    16381 ctggcgcgcc tgctgctcgg aatgctccag gtggcgccgc gggcggcctg cgttattggc
    16441 ggggacagca ctccccacat caagcctcat cccgatcccc tgctggccgc gtgtagcgcc
    16501 ataggcgaga gtcccggcgc ctgcatttac gtcggcgatg accagcgcga tatcgaagcg
    16561 gggcgtgccg ccggcatgaa ggtggcggcc gtcaactggg gctacctcaa cggcggcgat
    16621 ccggaaagct ggaatgccga ctggatgctc gatacgcccc aggatttgct gaaatacctt
    16681 ccgtagcaat cggcgttcag cacccagcta tcagcttgcg gcacggcacg ctgaaggact
    16741 tgatttccct gccggtaatc cacatatggg ataatgttgc cgctactcac cgtatttggc
    16801 tgacagctga atgctgacgg ctcaaatggg ggcgacctgg cttcgacgcg ggtaacgaag
    16861 cagttcgggg catgccaagg tccagtcacc ttgtaaatcc gctggaaact ataaacgcca
    16921 acgacgagcg ttacgctcta gccgcctaat accggctaga cgctgcacta gcttgctgat
    16981 gggctaggcc gggaaaccgg tgcagcgtca ttgacatcag cccctccgcg tggtttggtc
    17041 gcttggccgc acggatccgt cgaagcgact ggctgagcgc cagcctgccg gccggcgggc
    17101 gcaaggcgag acccaaacga ccaggctaag catgtagtcc cggctgtgta ggacttgcgg
    17161 acggcggttc gattccgccc gcctccacca attcaacggc cacccccggg tggccgtttt
    17221 tctgtacacc aaatgaaaag gggctcctga gagccccttc cttttcgctg tgaaaccgcg
    17281 attagagtgg cttgatcgaa gccgccttca ggcccttggg tccgcgcgcc acttcgtagc
    17341 tgacgcgctg cttttccttc agcgacttat ggccgttgcc ctggatctcg gaaaaatgcg
    17401 cgaacacgtc ttcaccgccg tcatcaggcg tgatgaagcc gaagcccttc gcatcgttga
    17461 accacttcac ggtaccggtt gccatacaaa acaatctcct atacaaaaat ggaatgaaca
    17521 cgggccttga gcccgcactg cgcaaacgaa tcaagaagtg gttgagaact ggagacactc
    17581 cacccggacg ggggaagcac taggtcagca atacgccgct tgaaacgact gcggggcgaa
    17641 cgatacccgc attcgcgcaa aatggcaagc cgggcgatta cccggtagtg tctcagtttg
    17701 acttgtaaca gtgagatttc agcattgagt gcaaacttta agggttgggc caccgcttat
    17761 atccgtttcg taatctagcc gcccaccttc aacgtgaacg ctcagctgtc cacccaaaac
    17821 cctaacgggc attgtgcttt gcgcccatac ttccggaata gcctttctga gcaagctatc
    17881 cgcaactatt aatgttaagt tcacggcgat aattgtcaga attactttgg tatagcggtc
    17941 tatcattgaa gtccctccct ttaatcgtgt tacaattgcc gctcatcaat tctgtctagg
    18001 caacgggcag atggacagaa ctcgtaagtc aaatttggat aatctcgttc ccctttggga
    18061 gcgaaaactc ccgcactccc tgcacgagcc cccacttgag agcttcatcg ggagtgatgt
    18121 ggacttccct tacgcgcctg gcgctaagca tctcgttcgg gaggccggtc ctctcgcgca
    18181 agatattttc agtgcgctgg tcctcagcca acgccgcttt aagggccgcg tctagccttt
    18241 cccaaggaac tgcttcctga aacggcccga tagttgtcgg gtgaatcatg aatataccgt
    18301 gaggggagca aagtcgctct gccgctccta caaaaaccgc tactgcaact gaactaatac
    18361 ttccggtgtt gtgggtggtg atttttatcg gtagtcccct aatatggttg tagagatata
    18421 cgccatcacc tacgtaacct cctagagaac tgagacacaa gtaaacctca tcgtatccat
    18481 tgttcacagc agcatttagg gcggccgcaa ctcttgtgac tccacttgaa tcaatgattc
    18541 cggcgtatga gtagtaagca actttctttc cggcaagatc agtcatttcc ccccccttaa
    18601 ttcgctagtt tagcgatttc ttccagcgtc caaacgtgat ctgatacccc cgcttgcatt
    18661 gcaggagtaa cccgcagcgt cttatgaatg cgaccgaagt tgtaatgcat gtagtgcatc
    18721 gccacggcat gctcatggtt ctcaaccttc ttggagaatc cgttcgtcag ccgcgtgaac
    18781 cggcgcatgg acatacgcat cgtaaggttc tgccgctcca caaatgaggt tgaaacgtgc
    18841 gccgggtcag gatcgccgca caccgtcccg gtgatcgtgc cgcagcactt ccctgaacta
    18901 tagcgccgct cttgctggtt tccctcagcc gcgccataga gtttcaccag catgctgtaa
    18961 tcaacttctg agccgaaagc atcctctacc gcagagaggt aggctttatg cccatcgctg
    19021 gttaattgag tgcggccttt gatgcgagag gccagatcgt taatgaatac ctgcgcgtat
    19081 tcagcatccc gcgttcccac cagccaagat ggaaccagct tagtatcggc gtccaacgcc
    19141 acccaggtat aaacgtcacc ctgtccgaaa acgccctgac ggttgggcgc tacgttcttt
    19201 tgcttcatgc cgacgaatga ccaaatctca tcaacctgca cgcgcctgac ggtcaggtta
    19261 cgcaatattt cgttctggta gatagcgcag gctgttccca cgtccaccaa cagcttggtg
    19321 accgtgttga tgctgcaacc aatcatccgg accgtagcgc gtaggctgtt accctcggta
    19381 agacatccga gtattctggc gcggtcttgg aggctcaagc ggttcatact gaacattatg
    19441 cttgagaggg agaggggctg tcaagcaatt tataataata ttattatata ccggcatccc
    19501 ccaaacggcg cttgcaacca gataactttt ttgttatata atccagtgac ctattggatt
    19561 tttatggatt acgtgtcgga ggcgggcacg aaccaggtca agcgatggtc taaaaagacc
    19621 ctgaccgatc aggagcgctc cgacatggaa accctgctgt ctgtgctggc aaaacagaag
    19681 cagtggcaaa tgccggattt caagacgttg tcgaatatgc agggcttggg ggaaattcga
    19741 tggaggtcgc cacaacgcac accactccgg ctcataggta tgcgtggcga aggtgctcaa
    19801 tacatcttgc taatcgggtg ctctcacaag ggcacggtct atacacccgc tgatgcgctg
    19861 gaaaccgcac tgaaacgcaa gagatcgctg gctaacggaa cggggagcat ttgtgagcac
    19921 gaagaggacg atggaacgac tgaagaggag caagcccaat agggctgcct tcgtcgcatc
    19981 ccaaatcaat attgggattc cttttcagat aagagcgctg cgtaagcagc gcgaatggga
    20041 ccagaagaag ctggcccatg aatctgggat gttgcaaccc aggatttccg ctatggaaaa
    20101 acctggctac ggcagcctaa ccttggagac tctaaagcgg cttgccgcag cttttgacgt
    20161 tgctctggtt gttcgctttg cgccgtttag cgaattggtt agatggcatg acaagttttc
    20221 gccggatgag tttgcaattc ccgattttga tgaagagcta tcaagcgtat ctgagagctc
    20281 ggaaggaaac gtaatcgcgt ttaactacct tagtattgtt gatgccaaag tcacttccct
    20341 acccggagcc aaggcggaag cgaatgtcgc cacgaccgaa gggaccagtg tcgctttcaa
    20401 acaggtagct tgatagtaga ggtattacaa catggcagac attaaagagc agatcgcaaa
    20461 gctcgccgca gccattcccc ttgctaaagg gcaagatcat agagtcacct atgcaaattc
    20521 cagcaggatg ggcatatcgc cttgggatgt ccgaattatt ttcggacaag ttattgaggc
    20581 agctaacgga acccaagtta acgaggacca aactactgtc gtgatggcgc cggcgcttgc
    20641 aaagcaggta ctaaagaacc tggaaagcac ggtagcccaa tacgaggagc tgttcgggga
    20701 gattaaagac ccgatggcca aaagcggatt ggcgcaggga gcacaacaac cacaacaaca
    20761 aaagacgaga aaaccgccta agcttaagaa gctctcttag acttccaccg ggaaaccgct
    20821 gctttcttag cgatagccga gcgttgctct tgagagagcg cgccagccct agccctacct
    20881 ccttttgcgc cgcccaatct gccgcgtagg acggctgctt ggttcttgtt ctcgtcaggg
    20941 tctagctctg agacatcccc tattgacaga tccactatca gtttcgccaa ctgattgggg
    21001 tcacgcgggc gcttgggacg ctttgccatg ccccatttta tcccgcatca tcccggctag
    21061 tcccgaattg ttacgatcaa actgagacac taccgattac cctagcgttt ttcagaattt
    21121 ggcaagtatg gccacgggtc taggtcccgg ccgcgccggg cccgccgcag atgagtgcgc
    21181 gtatctcctt gcgccgcggg ggcgaggcgg gattgatgcg gaccaagcgt atgccggccg
    21241 tcttgaggca atcggacttg aagcgttgtg ttccggcggc atccgcaccg cccggcgctt
    21301 ccacctcgac cacggcggtg atccgcatgt cccggtcgca gaccacgaaa tcgagacggt
    21361 actgcgatag acgccgcacc tgctgctcgc gctcggattc cccgcccggc gcgccgacaa
    21421 cagacgccag cgacacgttc gcgaagacct catggtcggg aagcccggtc ttgagcaaaa
    21481 aatagatcag cgtttctgac cgtccaagga acctctcccc ggccggtcgt gtcgcggcag
    21541 ccaccggcgc tgtcggcatg gaaccaacgc cggtatccgg aacgcctgcc ggatttgatt
    21601 cagatgccgg cgcggtggat gccttcgtct tgaaaatccg ctcgaaacgc tccttgctga
    21661 cggattctct cagggcagcc ttcttgcggt aattccagac gacggcggcg gctatgaatg
    21721 ccaccgccag caggaacaga aaccacgcca aagacggtct ctcaggcagt cctgctgcca
    21781 gccgtgtcga gcagcttgag ccccggccgc acggggtcgg ccgcacgcag cgggaaatcc
    21841 ggcgcgagca ggaactccgg cgagagctcg gcgatgctgc ggcagccgat cagcgccatc
    21901 acgcggtcga tctcttccct gaaaatcgtg accgcccgct tcgcgccttc ctcaccgcca
    21961 gccgcgacgc cgtagagcgt cgagcgtccg atctgaaccg catgcgcccc catcgccagc
    22021 gccttgacca cgtcggagcc gcggcggaaa ccgctgtcca ccagcaccgt gatgcgcttg
    22081 ccgaccgcgt ccacgatatc cggcagcgcc tcgagcggcg aaatcgcccc gtcgaggttg
    22141 cgcccgccgt ggttcgagac gattacgccg tcagcgccat tgtcagccgc gagcactgcg
    22201 tcctgcgggt gcaggatgcc ctttaccatc agcttgtgcg gccacattct gcgcagcgct
    22261 ttcaggtcct cccagttgag cgagtcgcac ttcatcatgg agcgacccat cggccgggcg
    22321 gtgatccggt tcttgatctc ggacgggtag ttctcgtagc gcggcatgcc ggtcgtgagc
    22381 acgtagcggg cgagcacctg aagcagccag cgcgggtgca gcatcacgtc ggtcacgttg
    22441 cggtgggtga acttgaacgg gatggtgaag ccgttgcgga ggttgtattc ccggtttccg
    22501 gataccgcgc cgtccaccgt cacgaccaac gcctcgtagc cggcggcctt ggcgcgttcg
    22561 acgatcttgt gcgagaggct gcggtcgggc cacatgtaga gctggaacca cagcgtgccg
    22621 cctgcctgct cggcgaccgt ttccatcgcc gtcatggacc ccgtggcgag cgtgaacggg
    22681 atgcccgcct ccgccgcggc gcgcgcgagc ccgatctcgc cctggtacca catcaacccc
    22741 gccgtccccg tgggcgcgat cacgatcggc atcttgtgcc gctttccgaa cagcgtgatc
    22801 tcctgggtgc gcttcgagac gtccaccagc gtgcgcggct tgaagcggat gcgctcgaaa
    22861 atcacgcggt tatggcgcag cgacacctcg tcctcggtgc cgcggtccac gaactcgaac
    22921 agccccttcg gcacccgctt gagcgccatc tcccgcaggt ccgcaatgtt gtaggccttg
    22981 gctaccgatc ccatagtcca tctcctggaa cgcgtcccgg agctaccgcc ggtcgcggct
    23041 ggaatattaa gggaaaccgg aggagctcgc cagattccgt ggcgtgctca gcgcggcatc
    23101 gcccaggcgg acacccatcg ccacaggcgc cacgccgaaa acacacggcc ggccagagag
    23161 ggcaacccgc gccggctgaa cacgaccagc gcggcgacgg cggctcctac gagcaccgga
    23221 tgcgcttgga ggaagcgcgc aacttccagc ccgcggtcaa cgatgccgat tggaccttcc
    23281 agccggcgga tgctttcgcc gatcgccgcg cgctgcgctt cggcgcgcgc gatcagccgc
    23341 tccttgcgcc gcgcgatctc gatcaggcga tcctcggtcc ggttcatcgg gagatcaact
    23401 ggtccttgtc cttggccagc tcgtccagac tggcggaaaa cagcctgggc cgcgcgttca
    23461 ggcggttgcg tacgccgatc gccgtgccga tgccggccgc cagaaaaatc ccggcaagca
    23521 ccgcgatgga agcggtccgg taactgtccc agaacaccac tacgatgagc acggtcagca
    23581 acagcacgct gatggcgaag aagaacagcg ccgcggccgc ccagaacagc agctgcagaa
    23641 accgtatgcg ttcctcttcg agctccgtgg cgagcagctc gaggcgggtc cgcaacagcg
    23701 caaccagcgt cgccgccagc ttgcgcaacg actgcacgat tccggcgccg gcttctccaa
    23761 gggcgggctc tggcatgacc gcggtccgct cagcgccggc tgatcagcag gccgatgatg
    23821 aagccgatgc cggcggcgat ccccaccgcc cgccatggat gatcgtgcac gtattcgtcg
    23881 gtggcgcgcg cggcctgctt gctcttgtcg atgatgacgt cctcgacgtc ggcgagcttg
    23941 accttcgcgc gattcaggct ttcctggatc ttctcgcgcg cggcggagac cttttcgccc
    24001 gcctggctgg cggtggcacg cagcagttcc tcggcgtcgg aaatcacgat cttgaggtcc
    24061 tgcacgagct tttccttggt gacggcggaa tcgggagtca tggtgtcctc ttgacagggg
    24121 gg
//
LOCUS       DBNGWZ010000367         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_296273, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000367 DBNGWZ010000000
VERSION     DBNGWZ010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_296273"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC16CX_10550"
     CDS             <1..912
                     /locus_tag="AC16CX_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012986.1"
                     /translation="LAQPYPNRPVRLIAAQSAGSSLDTITRIITPRLSETLGQQLVID
                     NRGGAGGIIGVELAARATPDGYTVLVGAPSSMILSKFTYTKLAFDSAKDFDPVSLTVN
                     AESVMVVHPSLPAKSVKEFIALARAKPRSLNMASAGVGASSHLAGIMFTNLSGIDTVH
                     VPYKGGGPMAAAVVAGEAQWAVSPAAALMGHIKAGRLRALAISSASRSPLMPELPTIA
                     EAGVPGYEYTSWNGIFVPRGTPRAIIAKLHATTQKVLAELDVRQQYAYQGLVPLGSAS
                     PEEFVKFFHADFSRIARLVKVAGIKPE"
     gene            complement(1013..2092)
                     /locus_tag="AC16CX_10555"
     CDS             complement(1013..2092)
                     /locus_tag="AC16CX_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3012987.1"
                     /translation="MAKPAVRRQEKWVPLAKEQFRERFFSKFYDPAFDKVRPELEKVY
                     EVAWDGYIVYRKSPRTKPAGKGFAEPSFKVAAEWLETRAKIEAAEKRQKDPKSPSRIL
                     VVNGSTRSEHTCPGEISKTRRLAQHAQKAVEKLPGYEVDFLDISTLADEPWKVIHPCK
                     ACVSTSQPLCHWPCTCYPNHALGQTNDWMAEIYERWVAAHGVMILCPVHWYQAPASLK
                     LMIDRLVCADGGNPDPTSTHGKDPAMAKALELKGWDYPKHLAGRAFSIVAHGDAAGPE
                     NLRRMLADWLTDIGMISAGPSAVMDTWIGWYQPYATSHEDLDRDKDLFTEVDNAALAL
                     ANMVKQIRTGKYHAPDEELHDPREK"
     gene            complement(2346..>2695)
                     /locus_tag="AC16CX_10560"
     CDS             complement(2346..>2695)
                     /locus_tag="AC16CX_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease/endonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3012988.1"
                     /translation="VRQLKRIADELTRRVREGEPAFFMGDMNDAWHPQRILMQAGFMS
                     CFAALGMQSPPTFQCYPTANVQPGERVITEAIDLIVANKYARAIAASVPQCYAGDLAP
                     SDHWPVQAIYQFV"
BASE COUNT          490 a          863 c          828 g          514 t
ORIGIN      
        1 ctggcgcagc cctatcccaa caggccggtg cggctgatcg cggcccagtc ggctggctcg
       61 tcgctcgaca cgatcacccg catcatcacc cccagactgt ccgaaacgct tggccagcag
      121 ctcgtgatcg acaaccgcgg cggcgcgggc ggcatcatcg gcgtcgagct cgccgcgcgg
      181 gccaccccgg acggctacac ggtgctggtc ggggcgccga gctcgatgat tctttcgaag
      241 ttcacctaca cgaagctcgc cttcgactca gccaaggatt tcgaccccgt atcccttacg
      301 gtgaacgcgg aaagcgtgat ggtggttcat cccagcctgc ccgcgaagag cgtaaaggag
      361 ttcatcgcgc tcgccagggc caagccgcgg agtctcaaca tggcttcagc gggggtcggc
      421 gcctcgagcc atctggccgg catcatgttc acgaatctct ccggcatcga taccgtgcat
      481 gtgccctaca agggtggcgg accaatggcg gccgcagtgg tcgccggaga ggcgcaatgg
      541 gcagtcagcc cggccgcggc gctgatgggg cacatcaagg ccgggcgcct gagggcgctt
      601 gcgatttcct ccgcgtcgcg ctcgccgctg atgcccgagc tcccgaccat cgccgaggcg
      661 ggtgtgcctg gctacgaata caccagctgg aacggcatct tcgtgccgag aggcacgccg
      721 cgcgccatca tcgcgaaact gcatgccacg acccagaagg tgctcgctga gctggacgtg
      781 aggcagcagt acgcctacca gggcctagtg ccgctcggca gcgcgagccc cgaggaattc
      841 gtaaaattct tccacgcgga tttcagtcgc atcgccaggc tggttaaggt ggcgggaatc
      901 aagcctgaat agatgcagtg actaggtgac tgagtgatta agcgactgag tgtcgcctgc
      961 tgatgatgtt gatcttctca gtcacttggt tacctagtca cttagtcacc agttattttt
     1021 cgcggggatc gtgcaattcc tcgtccggcg cgtgatactt cccggtgcgg atctgcttca
     1081 ccatgttggc cagcgccagc gcggcgttgt ccacctcggt gaagaggtcc ttgtccctgt
     1141 cgaggtcctc gtggctggtc gcatagggct ggtaccagcc gatccaggtg tccatcaccg
     1201 cgcttggccc ggcggaaatc atcccgatgt cggtgagcca gtcggccagc atgcggcgca
     1261 ggttttcggg gccggccgca tcgccgtgcg cgacgatgga aaacgcgcgc ccggcgagat
     1321 gcttgggata gtcccagccc ttcagctcga gcgccttcgc cattgccgga tccttgccgt
     1381 gagtggacgt cggatcggga ttcccgccgt ccgcgcacac cagccggtcg atcatgagct
     1441 tgaggctcgc cggggcctgg taccagtgca ccgggcacag gatcatgacg ccgtgcgcgg
     1501 ccacccagcg ttcatagatc tcggccatcc agtcgttggt ctggcccagc gcatggtttg
     1561 ggtagcacgt gcaaggccag tggcagagcg gctgtgaggt ggaaacgcac gccttgcagg
     1621 gatgaatcac tttccacggc tcgtccgcaa gcgtcgagat gtcgaggaaa tcgacctcgt
     1681 agccgggcag cttttcgacc gccttttgcg cgtgctgcgc cagccgccgc gtcttcgaaa
     1741 tctctccggg acaggtgtgc tcgctgcggg tggagccgtt cacgaccagg atccgcgatg
     1801 gcgatttggg atccttctgt cgcttctccg cggcctcgat cttcgcccgc gtctcgagcc
     1861 actccgcggc gaccttgaac gacggctccg caaacccctt tccggccggc ttcgtgcggg
     1921 gcgacttgcg gtagacgata tagccgtccc acgcgacctc gtacaccttc tctagctcgg
     1981 gacgcacctt gtcgaaggct ggatcgtaga acttcgaaaa aaagcgctcg cggaactgct
     2041 ccttggcgag tggcacccac ttttcctgac gacgcacggc tggcttggcc atcttatgtc
     2101 tctccctggt gatgagtgat gggtgatggg taatgcgtca cccctcccgg ccaatgatac
     2161 ggcctggcgg gcaaatcaga tgccacggct gaaaccggaa aacagccacc aaggcgccaa
     2221 gaacgcaaga aaatctagac gagaataaga taaaaagacc agcgaccgac atagccgcaa
     2281 gaacctgtaa cccatgtccc ccgacgtttt tgtcacctat ctccccggcc gctcatcgcg
     2341 gcgactcata caaactggta tatcgcctgc acgggccaat gatccgacgg tgcgaggtct
     2401 ccagcgtagc actgcggaac gcttgcagcg atcgcgcgcg catacttgtt cgcgacaatg
     2461 agatcgatcg cctcggtgat cacccgctcc ccgggttgaa cgttcgccgt cggatagcac
     2521 tgaaaggtgg gcgggctctg cattcccagg gcggcgaagc agctcataaa tcccgcctgc
     2581 atgaggatgc gctggggatg ccaggcgtcg ttcatgtccc ccatgaaaaa tgccggctcg
     2641 ccttcacgca cgcgtcgcgt gagctcgtcg gcaattcgct tgagctgccg caccc
//
LOCUS       DBNGWZ010000368         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2814316, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000368 DBNGWZ010000000
VERSION     DBNGWZ010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2814316"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /gene="trmD"
                     /locus_tag="AC16CX_10565"
     CDS             <1..744
                     /gene="trmD"
                     /locus_tag="AC16CX_10565"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR3012989.1"
                     /translation="VITLFPQMFEAVTGWGITGRAREKGLYQLVLWNPRDYATNSYRT
                     VDDRPYGGGPGMVMMVEPLEKAVSAARQRQQSAGAKRTRVAYLSPQGRLFDHRLVMEF
                     AGGDGLVLLTGRYEGVDERLVKRQVDEEISIGDYVLSGGELAAMVVIDAVVRQMPGVL
                     GDAESAGQDSFATGLLDCPHYTRPEVFEGEAVPQVLLSGNHAEIRRWRLKQALGRTWQ
                     RRPDLLEKRALTDEERALLDEYRKEAQGA"
     gene            747..1136
                     /gene="rplS"
                     /locus_tag="AC16CX_10570"
     CDS             747..1136
                     /gene="rplS"
                     /locus_tag="AC16CX_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR3012990.1"
                     /translation="MDIIRQLEAEEVKRLGREIPEFSTGDTVVVNVNVVEGDRKRLQA
                     YEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQTYSPLIASIEVKRRGDVKRAKLYHL
                     RNRAGKAARIRERLEGGEEGAPGGTEE"
     gene            1339..>2909
                     /locus_tag="AC16CX_10575"
     CDS             1339..>2909
                     /locus_tag="AC16CX_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3012991.1"
                     /translation="MRVILALICIACAGASSALETARSLAASGAPHLALARVEQLQPP
                     GTGAAGWAEWEALRLTLLTELKRYDEALKRAAALPADAPQPALRQCLLAAARAGVAAG
                     QGATARAYAARLLWQLDATPEETRAARLLVIESHLAERQGEAAFRAMLRFEQDYRPLG
                     RNVAERFVEALLDLGLEKEAVNWLAGLDDAGALKLRLRLKAGLTAPDAAIAQARAQLA
                     KGGGADYWRVLAEAAARQGNSALRIEALERLLNQDGGSDARGQRALASELWRAYLSEA
                     QAAVNRNRLLTGDDAAWLDFAARQLGASPHLSRALYGHLARNGAARETRNIAQLQLAF
                     SLYQNGFDHAALHLFGDESLKPEALDPQARYLLGTIAETRNAPALAVRFWQGLAAPPD
                     AGAEEWQVRLAAVQWRAGMPEAAAGTMHALMKPARPLPDSTANRAFTLAREMRDGGKP
                     DLAQELLVALLPLAGREQAREMLFALGGIAESAAQFGRAADYFLRAALAGESQTADAL
                     ALRARLAAGMNLARAG"
BASE COUNT          468 a          969 c         1062 g          410 t
ORIGIN      
        1 gtcatcacgc tgttcccgca gatgttcgag gccgtgaccg gatggggcat caccggccgc
       61 gcgcgggaaa aggggctgta ccagctcgtg ctgtggaacc cgcgcgatta cgcgacgaac
      121 agctaccgca ccgtggatga ccggccgtac ggcgggggcc ccggcatggt gatgatggtg
      181 gaaccgctgg agaaagcggt ttcggcggcg cgccagcggc agcagagcgc gggagcaaaa
      241 cgcacgcgcg ttgcttatct ctcgccgcag ggacggttgt tcgaccaccg cctggtgatg
      301 gaattcgcgg gcggggacgg gctggtgctg ctcacgggac gctacgaggg cgtggacgag
      361 cgcctggtga agcgccaggt cgacgaggaa atctcgatcg gtgactacgt gctctcgggc
      421 ggagagctcg cggcgatggt ggtgatcgac gcggtcgtgc ggcagatgcc gggcgtgctg
      481 ggcgatgccg agtcggctgg gcaggactcg ttcgcgaccg gcctgctcga ctgcccgcac
      541 tacacccgcc ccgaggtgtt cgaaggcgag gcggtgccgc aggttctgct ctcgggcaac
      601 cacgccgaga tccggcgctg gcggctgaag caggcgctcg gtcgcacctg gcagaggcgg
      661 ccggacttgc tggagaagcg cgcgctcacc gatgaagagc gcgcgctgct ggacgaatac
      721 aggaaagagg cacaaggagc ctaaatatgg acatcatcag gcagctcgag gccgaagagg
      781 tcaagcgcct cggcagagag atacccgagt tctccaccgg cgataccgtg gtggtgaacg
      841 tcaacgtggt ggaaggcgac cgcaagcgcc tccaggccta cgagggcgtg gtgatcgcca
      901 agcgcaaccg cgggctcaat tcctccttca tcgtgcgcaa gatctcctcc ggcgaaggcg
      961 tggagcgcac gttccagacc tattcgccgc tgatcgccag catcgaggtc aagcgccgcg
     1021 gcgacgtgaa gcgcgccaag ctctaccatc ttcgcaaccg cgccggcaag gccgcgcgca
     1081 tccgcgagag gctcgaaggc ggtgaagaag gcgcgccggg cggaaccgaa gagtgaacgg
     1141 gagcacgacg ggaaaaaggc ggcgcaagcc gcctttttta ttgcccgtat gccagcgcgc
     1201 aaaaaagcgg catggccgct ttttttacgg ataccccgac cccggcgatg tcacgcctct
     1261 atcagctggc acgcactgcg tgtgttcctg gttgcgtgtt ctgacaaggc ggcgcccccc
     1321 gggggctaaa attggccgat gcgcgtaata cttgcactga tctgcatcgc ctgcgcgggc
     1381 gcctcctcgg cattggagac cgcgcgcagc ctcgccgctt ccggggcacc gcacctcgcg
     1441 ctcgcccggg tcgagcagct gcagccgccc ggcaccggcg cggcgggttg ggccgagtgg
     1501 gaagcgttgc gcctgaccct gctcaccgaa ttgaagcgtt acgacgaggc cctcaagcgc
     1561 gcggctgcat tgccggcaga cgcgccgcag ccggcgttgc gccagtgcct gctcgctgcc
     1621 gcgcgcgcgg gcgtggcggc ggggcagggc gcgacggcgc gcgcctatgc cgcgcgcctg
     1681 ttgtggcagc tcgatgcgac gcccgaggaa acgcgcgccg cgcggctgct ggtcatcgag
     1741 agccatctcg ccgagcgcca gggcgaagcc gcgttccgcg ccatgctgcg cttcgagcag
     1801 gactaccgac cgctcgggcg caacgtcgcc gagcgtttcg tggaggcgct gctcgacctc
     1861 gggctcgaaa aggaggcggt caactggctg gcgggtctgg acgacgcggg ggcgctcaag
     1921 ctccggctgc ggctcaaagc cggtctcact gcgcctgatg ccgccatcgc ccaggcgcgc
     1981 gcgcagctcg ccaagggcgg cggcgcggat tactggcgcg tgctcgcgga ggcggcggcg
     2041 cggcagggca acagcgcgct gcggatcgaa gcgctcgaac ggctgctgaa tcaggacggc
     2101 ggaagcgacg cgcgcgggca gcgggcgctg gcaagcgagc tgtggcgcgc atacctctcc
     2161 gaggcgcagg ccgcggtcaa ccggaaccgg ctgctgacgg gcgacgacgc cgcctggctg
     2221 gacttcgcgg cgcgccagct aggagcgagt ccccatctgt cgagggcgct ctacggccac
     2281 ctcgcccgca acggcgcggc gcgcgagacg cgcaacatcg cccagctgca actcgcattt
     2341 tccctttacc agaacgggtt cgaccatgcc gcgctgcatc ttttcggcga cgagagcctc
     2401 aagccggagg cgctcgatcc ccaggcgcgc tatctgctcg gcacgatcgc cgagacgcgg
     2461 aacgcgcccg cgctcgcagt caggttctgg caggggctcg ccgcgccgcc cgacgccggc
     2521 gcggaggagt ggcaggtgcg gcttgccgcc gtgcaatggc gcgccggcat gcccgaggcc
     2581 gccgccggca cgatgcacgc gctgatgaaa ccagccagac cgctgccgga ctcgacggca
     2641 aaccgcgcgt tcactctcgc ccgggagatg cgggatggcg gcaagcccga tctcgcgcag
     2701 gagttgctcg tggccctgct gccgctcgcg ggacgcgagc aggcgcgcga gatgcttttt
     2761 gcgttgggcg gaatcgcgga atccgcggcc cagttcgggc gcgccgcgga ctacttcctg
     2821 cgcgccgcgc tcgccggcga atcgcagacg gccgacgcgc tggcgctgcg ggcccggctt
     2881 gccgcgggga tgaacctcgc ccgcgccgg
//
LOCUS       DBNGWZ010000369        65491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_797239, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000369 DBNGWZ010000000
VERSION     DBNGWZ010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 65491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 65491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_797239"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1677)
                     /locus_tag="AC16CX_10580"
     CDS             complement(148..1677)
                     /locus_tag="AC16CX_10580"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3012992.1"
                     /translation="MALDASRPDLIVGVIGTGAMGRGIVQVAAAGGMRVLMTDSRPGA
                     ADEARDFVDKMLARAAEKGTITRDEAVAAVDRVQIVGGPADMKPCHMVIEAIVENLQA
                     KQELFAELESVVAPDCVLATNTSSLSVTTIASKLKTPGRLAGFHFFNPVPLMKLVEVI
                     EGLRTEGWVAEALMTIGRRMTREPVRLKDAPGFLVNQVGRGFTLEASHLVYEGIASFV
                     DVDRVMRDVGGFRMGPFELMELTGLDVTHPASELIYNQFFQEPRYRPNLIMRSRYEAG
                     VLGRKTRRGFYEYDAEMKPVLAPEPPAPAAYRGGVWVSPAEPKGHAVMTELLKKLGAR
                     LETGPKPAADALILVTPIGEDATTCAVEQGLDPKRTVAVDTLFPMAKRRTLMGTPLTG
                     DVYRQGAHGLLAADGIPVTVCRDSPGFIAQRIVAMIVNIGCSIAQSRTAQPADIDKAV
                     ALGLNYPNGPFKFGDVLGPDRVYKVLSGMYRVYGDPRYRPTIWLTRRARLGVSLQTPE
                     P"
     gene            complement(1713..2528)
                     /locus_tag="AC16CX_10585"
     CDS             complement(1713..2528)
                     /locus_tag="AC16CX_10585"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR3012993.1"
                     /translation="MNHPAAPIQTLTDKLIAKREGAIGWVIFNNPERHNATSYEMWQS
                     LPVVLETYVNDPEIRVIAFKGTGEKAFSAGADISQFKEKRTSAEAVKEYNAAADAANR
                     ALRECAKPTIAMIRGYCIGGGTAVAVGCDIRIAADDARFGVPAAKLGLGYRFEGIRRL
                     ASIVGPSFTAEIFFTARQFTAQEALQMGLVNHVVPTGELESYTLDYINTITVNAPLTI
                     AAVKRSLIELQRDAPQRDLAQCQRMVDACFESEDYKEGQAAFMDKRKPQFKGR"
     gene            2655..3623
                     /locus_tag="AC16CX_10590"
     CDS             2655..3623
                     /locus_tag="AC16CX_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3012994.1"
                     /translation="MRRLVMACAAIILGLACFAAPAQGYPSKPIRIIVTFPPGGPSDF
                     VARALAQKLTETWGQQVVVDNRSGAGGVVGVEAAARSSPDGYTLLQGQSGGMSINPAL
                     HAKLSYDPFRDFMPVSMLVINAQLLVAHPSLPVRSVKDLVALARHRPGQISYASAGYG
                     SAQHLGMEMLKVMTGIDLLHVPYKGTGPALIDLFSGQVSLQFTSMPAVLPHVKAGKLR
                     GIAVGSSKRSAAAPDIPTVSESGVSGFEYVAWYGLFAPAGTPQVLVNQINVHVVNALG
                     NVELAQRLASQGAEPSPSTPEGLAKYLREDHERWKKVIRSANIRIE"
     gene            complement(3668..4111)
                     /locus_tag="AC16CX_10595"
     CDS             complement(3668..4111)
                     /locus_tag="AC16CX_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3012995.1"
                     /translation="MFKHILLPTDGSTMSNKAVKRGIEFAKQSRARVTAIHVVPEFKL
                     MVDEGVTIFNSALKKRFEDEARTRAQKMLDGIARQARARGVRCATVSVASDLPYQQII
                     ATARKKKCDLIMMASHGRHGLSSLLLGSETAKVLTHSKIPVLVVR"
     gene            complement(4263..4712)
                     /locus_tag="AC16CX_10600"
     CDS             complement(4263..4712)
                     /locus_tag="AC16CX_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR3012996.1"
                     /translation="MIVFNLGCENSHRFEGWFASNEDFERQIGRKLISCPLCGNANVV
                     RLPHASHVKTGGKERSARTEPAAGTQQQYANVGSEALAKLIERIIENTDDVGAAFPEE
                     ARKIHYREAPERRIRGTASREEVESLKEEGIEVVALPIPAHRLAKTH"
     gene            complement(4737..4821)
                     /locus_tag="AC16CX_10605"
     tRNA            complement(4737..4821)
                     /locus_tag="AC16CX_10605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4785..4787),aa:Leu,seq:taa)
     gene            complement(4828..5364)
                     /locus_tag="AC16CX_10610"
     CDS             complement(4828..5364)
                     /locus_tag="AC16CX_10610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR3012997.1"
                     /translation="MAQVLRRLGLRAAGGNYELVKMRINALRLATEHWTGQAHLRGKT
                     NPHIRRYPLQDILRRGTYYQSNKLRKRLLREGVLEPKCSSCGGVEWLGRSIPLELDHV
                     DGDKTNNQLANIRLICPNCHALTPTYRGKNTKYPHIPAPEDVQEGIKRYGSMAAYARE
                     MSVSSDTVRSWLSRSAKG"
     gene            complement(5538..5611)
                     /locus_tag="AC16CX_10615"
     tRNA            complement(5538..5611)
                     /locus_tag="AC16CX_10615"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5577..5579),aa:Cys,seq:gca)
     gene            complement(5719..5794)
                     /locus_tag="AC16CX_10620"
     tRNA            complement(5719..5794)
                     /locus_tag="AC16CX_10620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5759..5761),aa:Gly,seq:gcc)
     gene            complement(5838..6407)
                     /gene="pgsA"
                     /locus_tag="AC16CX_10625"
     CDS             complement(5838..6407)
                     /gene="pgsA"
                     /locus_tag="AC16CX_10625"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR3012998.1"
                     /translation="MPFNLPNLLTWLRIILIPLFVGIFYFEKSWVSIPNQNLVATILF
                     TAAAVTDWLDGWLARKLNQMSAFGAFLDPVADKLMVAAALIILVQLERVDAIIALIII
                     GREIAISALREWMAQIGAAKSVAVSFLGKIKTTSQMLAIPLLLYHDSIGAFSPQQVGT
                     WLIYLAALLTLVSMAYYLKVALPQAWQRP"
     gene            complement(6541..8415)
                     /gene="uvrC"
                     /locus_tag="AC16CX_10630"
     CDS             complement(6541..8415)
                     /gene="uvrC"
                     /locus_tag="AC16CX_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3012999.1"
                     /translation="MVFDPATVVATLPHLPGVYRMLNSVGEVLYIGKALDLRQRVASY
                     FQKASGLPPRIQVMLEQVMAVETTVTRSEAEALLLESNLIKALSPRYNILFRDDKSYP
                     YLVITGHRYPRLGFHRGALDKRHRYYGPFPNAGAVRESIQLLQKVFRIRTCEDTVFSN
                     RSRPCLLHQIRRCTAPCVGLISEGDYAEDVGAAELFLAGKENEVIERLATRMHDVSAR
                     MNYEEAAVFRDRIAALRKVREKQFVSGEGDDADVVAWGRAEGAVCVNLVMIRGGRHLG
                     DKNFYPRNADECSAGQVIEAFVQQHYLQHGVPAQIVVGAKLDARPLEQLLSERAGRKV
                     RIHQHPAGVRRAWLEMAERNAQLGAAQALGLLATQEARLVALQQSLELPETARRIECF
                     DVSHIMGEATVAVCVVYDKSTLQKNEYRRYNIRVKTDDVSPLDSGAIERMQGAVHRAA
                     ITGGDDYAAMREALTRRYRKIVAGEGKMPDLVLIDGGKGQLAVAIDVFEELGMSDVMV
                     MAVAKGEDRRPGQEQLFLTGRANPLKLDAGHLGLHLIQQIRDEAHRFAIQGHRARRGK
                     TRNRSTLEKIAGVGAKRRQRLLARFGGLRGLLAAGVDELAQVEGISRTLAEKIYHELH
                     "
     gene            complement(8438..9112)
                     /locus_tag="AC16CX_10635"
     CDS             complement(8438..9112)
                     /locus_tag="AC16CX_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3013000.1"
                     /translation="MQEFTTACRRCPRLARYLDRIRDRHPDYHARPVPPFGPARAKLL
                     IVGLAPGLHGANRTGRPFTGDFAGILLYDTLHRYGFASRPVSVRAADGLRLENCRITN
                     AVRCLPPANRPLPDEVRRCNAYLAAEIRSMPDGAAILALGAIAHDAVLMALGLKRAAH
                     RFRHGALHALPRGLALYDSYHCSRYNTQTKRLTPEMFRSVFDAISRYLIHSRRRPRRR
                     TARGER"
     gene            9162..9728
                     /gene="efp"
                     /locus_tag="AC16CX_10640"
     CDS             9162..9728
                     /gene="efp"
                     /locus_tag="AC16CX_10640"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3013001.1"
                     /translation="MAKVLATEIRAGNLIEWDRRVWRVLKSYHVHVGGRGGAFMQIEM
                     KDIESGTKRNERIRTEDKIERAFVEPREMQYLYSDASGYVFMDKETFEQLTLPADFLE
                     GQTGYLIPSTDVQVNFHNERPIGVQLPPSVTLTVAETEPHLKSATATGSFKPARTETG
                     VTVMVPQFVMEGERIKVSTDTGEYIERA"
     gene            complement(9735..10631)
                     /locus_tag="AC16CX_10645"
     CDS             complement(9735..10631)
                     /locus_tag="AC16CX_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3013002.1"
                     /translation="MPVFLPALAITLAYALIELAGGLWTGSLALLGDAGHMFSDALAL
                     GLAAVAARLALRPVGHQHTYGWARAEVIGALLNSVLMLAIVVALVVEAVVRLHEPKPV
                     AAAGMVLIAFIGMLVNAAVAWLMSRGRVTLNVRAALLHVMGDLASSFAALLAGAVIYA
                     TGWLPIDPILSLVIAGLILAGTLKLLRDTLHVLMEGVPPAMELTAIGQTLAELPGVVS
                     VHDLHVWSIAPDKIALSAHIELDDLQRWPAILSTAGALLHDRYGIGHITLQPEVRDAP
                     GSRRTSVIRLWRGNSRPGSKFP"
     gene            complement(10664..11728)
                     /gene="nagZ"
                     /locus_tag="AC16CX_10650"
     CDS             complement(10664..11728)
                     /gene="nagZ"
                     /locus_tag="AC16CX_10650"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR3013003.1"
                     /translation="MAKTLALGPLMLDVSGTELTADDRRRLLHPLAGGVILFPRNYSS
                     SEQLMRLTAGIHALRNPPLIVAVDHEGGRVQRFREGFTAIPPMRELGRAWDTDPQQAR
                     QLASEAGFILAAELQAHGVDLTLAPVLDIDHGASSVIGDRALHSDPHAVAELASALLR
                     GFKQAGMSGVGKHFPGHGHVRGDSHHEVPVDERAYEEIEAADLAPFRRAIDNGLGGIM
                     PAHVIYPEVDSRPAGFSTVWLKQILRGRLGFGGVVFSDDLSMEGASAMGAVVERAEAA
                     LAAGCDMVLLCNNPAAVDELLGGLNYSMPAVSLARLARMHGRASAGGMIRLREDARYT
                     RALHAIAGIGLHDGELPLGS"
     gene            complement(11728..12105)
                     /gene="acpS"
                     /locus_tag="AC16CX_10655"
     CDS             complement(11728..12105)
                     /gene="acpS"
                     /locus_tag="AC16CX_10655"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3013004.1"
                     /translation="MIYGIGIDVVEPRRVARLMEKYGERFARRVLTPLEWPAYLRTRR
                     PVLFLANRFAAKEAFSKAMGTGFRYPVTLQCISVIPNTAGKPGFAFHPNLERLVTSRG
                     IVSHHLTISDEQNIACACVVLEA"
     gene            complement(12102..12824)
                     /gene="pdxJ"
                     /locus_tag="AC16CX_10660"
     CDS             complement(12102..12824)
                     /gene="pdxJ"
                     /locus_tag="AC16CX_10660"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR3013005.1"
                     /translation="MILLGVNIDHVATLRQARGTDYPSPVEAALAAETAGADYITLHL
                     REDRRHIQDRDVMVLRKVLKTRMNLEAAVTPQMIAFARRVRPQDVCLVPERRAELTTE
                     GGLDVARKSRRVERACRDLGAAGIRVSLFIDADKDQIDAALDAGAPVVELHTGAYADA
                     SAARRRGKELERIRAAAEYAASRGLRVNAGHGLNYRNVQPIAAIAQIRELNIGHAIVA
                     QSVFVGIDRAVREMKALIDSAR"
     gene            complement(12821..13552)
                     /gene="recO"
                     /locus_tag="AC16CX_10665"
     CDS             complement(12821..13552)
                     /gene="recO"
                     /locus_tag="AC16CX_10665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR3013006.1"
                     /translation="MAGPTDKSSQEARTAFVLHSYPFRETSLIVEAYSRDHGRVALVA
                     RGARRPKSSLRGVLLPFQPLLLSWAGRRELRTLTHAEWQGGYAPLKGRALICGFYLNE
                     LLLKLLPRDDPHERLFTIYQGTLAALGDGADQFALLRRFELELLRELGYAVILDRDVE
                     RDEPVARERNYVYVIERGPVRGGDAPRNGVELSGQTLIDMQSGIFASAVTQQQSRALM
                     RTLINHYLADQVLHTRQLLRDLQDL"
     gene            complement(13554..14411)
                     /gene="era"
                     /locus_tag="AC16CX_10670"
     CDS             complement(13554..14411)
                     /gene="era"
                     /locus_tag="AC16CX_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3013007.1"
                     /translation="MAIVGRPNVGKSTLLNRLVGCKLSITSSKPQTTRQRVTGIVTRS
                     DAQVVFIDTPGYQAGHGGALNRSMNRSVAASLQEVDVIVWMVEALKYDERDEKVSGLL
                     PARVPVVLAVNKTDSVKEKGRLLPFIDRLSKRREFEAIVPISAGNGSQVDELLEVVVS
                     LLPEGPPLHDADDLTTVDERFLAAELLREKLFRRLGDELPYSTTVEIERFREDGGLRH
                     IHASILVDKEGQRPIVIGTGGEKLKSIASQARRDMERLFGGRVFLEVRVKVRRGWAKS
                     DAMLKRLGF"
     gene            complement(14338..15111)
                     /gene="rnc"
                     /locus_tag="AC16CX_10675"
                     /pseudo
     CDS             complement(14338..15111)
                     /gene="rnc"
                     /locus_tag="AC16CX_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001013.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(15139..15498)
                     /locus_tag="AC16CX_10680"
     CDS             complement(15139..15498)
                     /locus_tag="AC16CX_10680"
                     /inference="COORDINATES: protein motif:HMM:NF027463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HIR3013008.1"
                     /translation="MHRQKGVSLSGFLMWAVVFIFAALLGFKIGPPYFEYLKIQKQMQ
                     AIADDPTLRSGQRRDIEESFVKRSTIEDINAISHKDLVITKEGDGFVLSAEYSVCVPI
                     VGNLKACMDFSPSSRIR"
     gene            complement(15511..16314)
                     /gene="lepB"
                     /locus_tag="AC16CX_10685"
     CDS             complement(15511..16314)
                     /gene="lepB"
                     /locus_tag="AC16CX_10685"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3013009.1"
                     /translation="MNFALVLFLLLVATGAIWLLDHYWLRRRRGPGRPEPWWVEYPKS
                     FFPVILVVFLLRSFLVEPFKIPSGSMLPTLLVGDFILVNKFTYGIRLPIINLKIADVK
                     QPRRGEVMVFRYPENPSLDYIKRVVGLPGDRIAYRNKRLWINGKEVKVAPDGEYNYIE
                     SGLNFVSAQRLIETLEEHRHAILVQSEVPSVHITGVRLFPFRDNCAYNETGFSCTVPE
                     EHYFMMGDNRDSSSDSRYWGFVPETNIVGKAFMIWWNFDDFKRIGQFIN"
     gene            complement(16411..18201)
                     /gene="lepA"
                     /locus_tag="AC16CX_10690"
     CDS             complement(16411..18201)
                     /gene="lepA"
                     /locus_tag="AC16CX_10690"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3013010.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRFIQLCGGLAEREMEEQVLDSM
                     ELERERGITIKAQTAALEYRAHDGRRYLLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYAAIELGVEVIPVLNKMDLPQAEPERVIGEIEDIIGLPARDALR
                     VSAKTGEGVAGILEAVISRIPPPRGDPDAPLKALIIDSWFDNYVGIVMLVRVVDGCLK
                     PKDRILLMSTGAGYLCEQLGVFTPRAKYKDVLAAGEVGFVTAGIRELKAARVGDTLTL
                     AQRPAAQPLPGFKEIKPQVFAGLYPVDEGDYGALRDALEKLHLNDASLRFEPESSQAL
                     GFGFRCGFLGLLHLDIVQERLEREYDMNLITTAPTVVYQVLTRDGTVMEIENPSRLPD
                     PARIAEIREPVIKASILVPQDYVGPVITLANQKRGIQRNLQYLGRQVMLTYELPLNEV
                     VMDFFDRLKSVSRGYASLDYEFLEYRAGDLVRLDILINGERVDALSQIIHRDNAQHRG
                     RDLAVRMRELIPRQMFDVAVQAAIGAHIIARETVKALRKNVLAKCYGGDVTRKRKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAILQVEK"
     gene            complement(18251..18511)
                     /locus_tag="AC16CX_10695"
     CDS             complement(18251..18511)
                     /locus_tag="AC16CX_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3013011.1"
                     /translation="MATEQRTLTLTVYSRNACHLCEEMIAALRNLQGRFRFEIEVVDV
                     DGDPALERLYGAKVPLLAHGKRELCHYVLERAAVTAYLAEFR"
     gene            complement(18501..19895)
                     /locus_tag="AC16CX_10700"
     CDS             complement(18501..19895)
                     /locus_tag="AC16CX_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3013012.1"
                     /translation="MFKRLQLRILVALFFIVPAAVAAQLPDFTELVEKQGPAVVNIST
                     TQSVRNPLLPQIPNLQEDDPFYEFFRRFIPQPGPREFQSQSLGSGFIISQDGYILTNA
                     HVVEAADEVTVKLNDKREFKAKVIGADRRTDVALIKIDASNLPAVRFGDPNRLRVGEW
                     VLAIGSPFGFENTVTAGIVSAKGRSLPQENYVPFIQTDVAVNPGNSGGPLFNLRGEVV
                     GINSQIYSRTGGFMGLSFAIPIDVANDIAQQLRTSGKVTRGRIGVVIQPVTKELADGF
                     GLSRLQGALVNSVEKGGPADKAGIEAGDVILRFDGKVVNASEDLPRVVGATKPGSRVT
                     LQIWRNKQARDLQVVVAEMQDDRAARQGRRGGKPPAASPSQYGLTLSDLTETQRAELK
                     VSGGVLVENAQGAAARAGIRRGDVILAINNQDVKSVEQFNQVMGQFDKGRIVALLIRR
                     GANSLYVPFRVDGN"
     gene            complement(19961..20941)
                     /locus_tag="AC16CX_10705"
     CDS             complement(19961..20941)
                     /locus_tag="AC16CX_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HIR3013013.1"
                     /translation="MKKAWFIVVLGLAAPVLAAETGAPQDGLAWLKKMASASRQLNYS
                     GTFVYRHPNQTETSRIVHYVNAAGGEFEKLETLEGPVREVIRTNDQVVCYLPASKTVI
                     IEKRSKRRFPAMLPEQLTGVSDNYVVNVDRKDRVAGHDCQVITLEPRDKLRYGHQFCA
                     DDASGLPLRARTFNEKNEQLESFVFTELKIGGSFNRDRVRSRYAAKSRNWKVDHAGFP
                     TTEIPAETGWVLTQQPAGFRKLTEQKRSIAGRAAMVSHIVYSDGLAAVSVFIEPLPKV
                     RPAQSLSHQGAVNIYIRPVSDYMVTVLGEAPAATVMQIANSLEFKGASPR"
     gene            complement(21044..21568)
                     /locus_tag="AC16CX_10710"
     CDS             complement(21044..21568)
                     /locus_tag="AC16CX_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015810.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HIR3013014.1"
                     /translation="MDKISALMDGELDKRQAHEQIARMKQDEELLYLWHAFHVAGDVM
                     RGERALSLNFSQDLIERLAKEPTVLAPRRSHLARRITAYALSAAASLSAVALVGWIAF
                     SNNPLAPQPELAKAQTAPPPVPTPSTQLASVPSDGKMNEYLIAHQEFSPSTAIQGLAP
                     YIRSVSSTQPATGR"
     gene            complement(21570..22169)
                     /gene="rpoE"
                     /locus_tag="AC16CX_10715"
     CDS             complement(21570..22169)
                     /gene="rpoE"
                     /locus_tag="AC16CX_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HIR3013015.1"
                     /translation="MSDREVDQQLVERAQRGDKKAFELLVSKYERKLARLLSRFIRDA
                     AEVEDVTQEAFIKAYRALPTFRGDSAFYTWLYRIGINTAKNYLVAMGRRAPTTTDIDS
                     EEAEGFENGDQLRDLNTPENELATRQIAETVNQTLDELPQELRTAITLREIEGLSYED
                     IANVMNCPIGTVRSRIFRAREAIAAKLRPLLGTRRDRRW"
     gene            complement(22388..23902)
                     /locus_tag="AC16CX_10720"
     CDS             complement(22388..23902)
                     /locus_tag="AC16CX_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HIR3013016.1"
                     /translation="MRLLPLLLFPALALAQPVINVGSKRFTESYILAEIVTRTLARPE
                     EARAVHHQGLGNTAILFAALKSGAIHAYPEYTGTIAFELLGLKSVPGPAGLNSELARH
                     GLAAGVPLGFSNSYALAMLESRAGALGVTRISDLSGHPQLRFALSQEFLNRGDGWRAL
                     QQRYQLPFVEARGLDHGLAYEALAAGQVDVVDVYSTDAKIARYRLRVLQDDRGFFPAY
                     DAVILYRADLPAKYPRSWRALQELTGRIDSEQMVRMNAAAELSALPFSRIAEDFLAGR
                     YSTGAAAPQRERRSFLSALFGADFWRLTAEHLLLVFASLALGVATGVPLGIWANRKPP
                     ARAWILGGAGILQTVPSLALLAFLIAALDRIGTVPAIIALFLYSLLPIVRNTETGLAG
                     VPRSLKDSATALGIGEGARLQLIELPLAARTILAGIKTSSVINVGTATIAAFIGAGGY
                     GERIVAGLAVNDHILLLAGAVPAAVMALGVQWGFDALDRLLIPPGLRQSGNRSG"
     gene            23992..25317
                     /gene="aptA"
                     /locus_tag="AC16CX_10725"
     CDS             23992..25317
                     /gene="aptA"
                     /locus_tag="AC16CX_10725"
                     /EC_number="2.6.1.-"
                     /EC_number="2.6.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     S-adenosyl-L-methionine:8-amino-7-oxononanoate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0016223 - beta-alanine:pyruvate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omega-aminotransferase AptA"
                     /protein_id="HIR3013017.1"
                     /translation="MPPRDYPLEPFWMPFTANRQFKAAPRILVSAKDMHYLAEDGRRI
                     LDGTAGLWCVNAGHCRPKIVEAIRRQAETLDYSPLFQMGHPGEFRAAALVAQLAPAGL
                     DHVFFTNSGSEAVDTALKIALAYHRARGEGHRNVLVGRERGYHGVGFGGISVGGIPAN
                     RKAFGNMLARVDHLPHTHYLEHNAFSRGQPAWGAHLADDLERIVALHDASNIAAVIVE
                     PVAGSTGVLVPPKGYLERLRAICDKHGLLLVFDEVITGFGRTGHAFAAQTFGVTPDMI
                     TFAKGVTSGTVPLAGVLVRRGIYEAILESAPPDTVELFHGYTYSGHPLATAAAEATLA
                     LYHEEKLFDRARELAPYFEQAVHSLRGASHVIDIRNFGLMAGIELEPLPGKPGARGHQ
                     VFLKCFEQGVMTRVTGDIVALSPPFIISRSQIDELAGTLGAAVRELEKK"
     gene            complement(25322..28852)
                     /locus_tag="AC16CX_10730"
     CDS             complement(25322..28852)
                     /locus_tag="AC16CX_10730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3013018.1"
                     /translation="MAADTIPVTGSGLAFDDLYRREGLLRLDRNFLTWLEQGDAGLHE
                     RLLRARAEPAALAPKQESELLLALAPHVEQFIARLFGIEAEVQVLAARHHELAPLYTV
                     KRQFVQRKAMHRIKPREASDYDGLALERELERAFGEPFTELAFARHVTQWQDDEAPHA
                     ARLETALRYAAWAVYSEAGRKRRRGGVLFKAPAKLDPQRLVPVVTEEAGGYKSHRLAA
                     DHLRRRDGFKLTDAGTDLTGALDEVNYCIWCHEQGKDSCSKGLREKGATGAGAASFKK
                     SPFGDTLAGCPLEEKISEFQQAKAEGHAIGALAIIVVDNPMVAATGHRICNDCMKSCI
                     YQKQEPVNIPQAETRTLKDVLELPWGFEIYSLLTRWNPLNLRAPYPKAPSGKRVLIAG
                     MGPAGIALAHYLMNDGHVAVGIDGLKIEPLPPTLSGVDARGARVPFQPVRDVRELYEA
                     LDRRVMAGFGGVAEYGITVRWDKNFLKILRLLLERRREFAMYGGVRLGGTLTAKEAFE
                     LGFDHVALAIGAGRPTVLELPNGLARGVRAASDFLMALQLSGAAKPGSIANMQLRLPV
                     VVVGGGLTAIDTATESLAYYPLQVEKFLQRYEKLAREHGETAARVAWDEEERRIADEF
                     LAHARALRSERMRAVAEDRAPRILELLQSWGGVTIAYRKRLIDSPSYTLNHEEVHKAL
                     EEGIRFAEGLSPLAIEVDEEGHARALKVSVQRRGENGKWAEIGQTELPARTILIAAGT
                     QPNTVLAREDPVNFRLDGRYFQACDENGDPVAVERAISKPARPRVLLSRRGDGRFMSY
                     FGDVHPSYFGNVVKALGSAKQGYPAVSRVLERRGPASMLSDDAFIARLNGELRATVHE
                     VVRLTPLIVEVVVRAPLAARRFRPGQFYRLQNYETSAREAEGTRLAMEGLALTGAWVD
                     RERGLVSTIVLEMGGSSDLCVLLKPGEPVVLMGPTGNPTEIPPGETVVLAGGGLGNAV
                     LFSIGQALRRAGSRVLYFAGYKHMTDRYKVHEIEAAADLVVWCCDEEPGFTPERPPDR
                     SFVGNIVQGMHAYASGALGEQPIAFKDADRLIAIGSDRMMAAVARARHDVLKPHLKAG
                     HVGIGSINSPMQCMMKEICAQCLQPHVDQATGKVSYVFSCFNQDQPLDRVDWKALEQR
                     LKQNSMQEKLTAEWLDHCLVALGERTMQRI"
     gene            29022..29168
                     /locus_tag="AC16CX_10735"
     CDS             29022..29168
                     /locus_tag="AC16CX_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013019.1"
                     /translation="MLDVRMSQGRRILLWLMLAVLAALVTYVAFRGYFSPELLLNFSN
                     TFSC"
     gene            complement(29183..30067)
                     /gene="folD"
                     /locus_tag="AC16CX_10740"
     CDS             complement(29183..30067)
                     /gene="folD"
                     /locus_tag="AC16CX_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR3013020.1"
                     /translation="MTASRCAAPAVVLDGAAAAREIYSRLKARVAALAGSGVRPGLAA
                     ILVGDNPASKIYLRNKVRACDEAGVHSEVHNLPADCPQEALLATLESLNRNPRIHGIL
                     LQLPLPAHLDAGRVMQAMAPEKDVDGLTWASLGALVAGRPSFEPCTPGGVIVLLERAG
                     VAVDGRDAVVVGRSPIVGKPMALMLMARGATVTICHSRTRDLAEHTRRADILVAAAGR
                     PRLIRDDMVKPGAAVIDVGINRLPDGKFAGDVDFEAVRRVAGWITPVPGGVGPMTVAM
                     VIVNTVRAAERSLTARAA"
     gene            complement(30069..30377)
                     /gene="ftsB"
                     /locus_tag="AC16CX_10745"
     CDS             complement(30069..30377)
                     /gene="ftsB"
                     /locus_tag="AC16CX_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HIR3013021.1"
                     /translation="MRVLALALAALIVLIQYPLWLGKGSWLRVWEVEQQIRAQRETNR
                     QLQARNNALEAEVRDLKVGLEAIEERARSELGMIRQDEIFIQVLEAAPGARASGPARP
                     "
     gene            complement(30513..31796)
                     /gene="eno"
                     /locus_tag="AC16CX_10750"
     CDS             complement(30513..31796)
                     /gene="eno"
                     /locus_tag="AC16CX_10750"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3013022.1"
                     /translation="MSAIVDVIAREILDSRGYPTVEADVLLESGVLGRSAVPSGASTG
                     SREAIELRDGDAARYGGKGVLKAVENINTEICEAVIGVDATEQSFVDKTLIDLDGTEN
                     KSRLGANAVLAVSLAVAKAAAEESGLPLHRYLGGVGAMSMPVPMMNVINGGAHADNSL
                     DMQEFMIVPVGFASFREALRCGAEVFHALKKLLAGRGLSTAVGDEGGFAPRLPKHEAA
                     IKIVLEAVEAAGYRPAEQVALALDCASSGFFEDGEYTLASEGLSMSAEQFVDYLAAWV
                     EKYPIISIEDGMAEDDWDGWKLLTRRLGRKVQLVGDDVFVTNTRFLKQGIEKGVANSI
                     LIKVNQIGTLTETFAAVEMAKRAGYTAVVSHRSGETEDTTIADIAVAANALQIKTGSL
                     SRSDRLAKYNQLLRIEEDLGETASYPGRAAFYQLR"
     gene            complement(31825..32679)
                     /gene="kdsA"
                     /locus_tag="AC16CX_10755"
     CDS             complement(31825..32679)
                     /gene="kdsA"
                     /locus_tag="AC16CX_10755"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR3013023.1"
                     /translation="MELCGFKAGLDQPLFLIAGPCVVESRDLALDTAGELKEICSQVG
                     LPLIYKSSYDKANRTSIKSFRGLGMEKGLQILDEVRNKIGVPVLTDVHEIDEIAAAAA
                     TADVLQTPAFLCRQTDFICAVAAAGKPVNIKKGQFLAPGDMKNVVEKAKAASRRDNIL
                     VCERGVSFGYNNLVSDMRSLMIMRNTGCPVVFDATHSVQLPGGQGSASGGQREFVPVL
                     ARAAVASGIAGLFMETHPNPEKALSDGPNAWPLKLMKPLLETLKALDATVKSRGFAEN
                     LLSGQPRC"
     gene            complement(32763..34439)
                     /locus_tag="AC16CX_10760"
     CDS             complement(32763..34439)
                     /locus_tag="AC16CX_10760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3013024.1"
                     /translation="MTTKYIFVTGGVVSSLGKGIAAASLAAILESRGLKISMVKLDPY
                     LNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFLHVKMSKRNNFTTGQIYESVIKK
                     ERRGDYLGGTVQVIPHITDEIKHFIRRGAAETEVAIVEIGGTVGDIESLPFLEAIRQM
                     AIELGRDNYCFIHPTLVPYVAAAGEIKTKPTQHSVKELREIGIQPDVLLCRADRKLPD
                     DERRKIALFTNVAVEAVISAVDVDSIYKIPAMLHAQKLDDIVCRKLGLNPPPANLSSW
                     ERLVHALENPQRTVNIALVGKYVDLTESYKSLTEALIHAGIHTGARVKIHYVDSEGIE
                     KNGTGCLGAMDAILVPGGFGKRGVEGKIKAIRYARENGVPYLGICLGMQLAIIEYARN
                     AAGLVGAHSTEFDPDTPHPVIALITEWQDRDGRIERRSAQSDLGGTMRLGGQECVLEP
                     DSAVRRIYGQERIMERHRHRYEVNNHYLPRLKSAGLKVSGVSAKDELCEMIELPGHPW
                     FVGCQFHPEFTSTPRHGHPLFKAFVQAALTYARPGEAGLAGDTGRLKSIA"
     gene            complement(34527..36920)
                     /gene="ppsA"
                     /locus_tag="AC16CX_10765"
     CDS             complement(34527..36920)
                     /gene="ppsA"
                     /locus_tag="AC16CX_10765"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HIR3013025.1"
                     /translation="MSNPPLVIDLATLRMNDVASVGGKNASLGEMISQLGAAGVRVPG
                     GFATTARAYREFLAQKKLDARIADRLGKLDVGDVAALARAGEEIRGWIVAQDFPQRLH
                     EEIAQAYERLADGAGADASFAVRSSATAEDLPDASFAGQQETFLNVHGLDNLYVAVKR
                     VFASLYNDRAISYRVHKGYLHDEIALSAGIQRMVRSDLGASGVIFTLDTESGFDQVVF
                     ITAAYGLGETVVQGKVNPDEFYVYKRALRDGKHAILRRGLGSKAVKMVYVDAPQPGRS
                     VGTVDVPERERRRFSISDAEVEELARYAMIIEDHYRRPMDIEWGKDGIDGRLYVLQAR
                     PETVKAGEKLDTLRRYRLKQRSEVLTTGRAIGQKIGAGPVRVVRKTDEMSAVRQGDVL
                     VTDMTDPDWEPVMKRAAAIVTNRGGRTCHAAIIARELGIPAVVGCGDATQVLKDGRPV
                     TVSCAEGDTGFVYRGVLETEVIDLSLSSMPKAPVKITMNVGNPELAFEFQRLPNEGVG
                     LARLEFIIARMIGVHPKAVLAYPDLPADVKVAVEEHSAGYPDPVTFYVDKLAEGVATL
                     GAAFWPKPVIVRLSDFKSNEYSSLTGGARYEPREENPMLGFRGAGRYVDPSFRDCFEL
                     ECRALKKVRDEMGLTNIEIMVPFVRTVNEAKKVLELLAGNGLRRGENGLRVVMMCEIP
                     SNAILADQFLEHFDGFSIGSNDMTQLTLGLDRDSSIIAEQFDERDPAVKQMLHLAIQA
                     CRRAGKYVGICGQGPSDHPDLAKWLVEEGIESLSLNPDSVVETWLYLAREAGSSRKT"
     gene            36993..37817
                     /gene="ppsR"
                     /locus_tag="AC16CX_10770"
     CDS             36993..37817
                     /gene="ppsR"
                     /locus_tag="AC16CX_10770"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HIR3013026.1"
                     /translation="MTPKRTAFFVSDRTGITAEMLGHSLLTQFDGVRFYEVTLPFVDT
                     VEKAREAVERINQQGATDGVRPLVISTLARTEIAQVIGQSNALFLDCFEIFISPLERE
                     LGIKASHAVGRTHSISDFVNYHHRIESVNYSLAHDDGVSYRDLNDADVILVGVSRCGK
                     TPTCLYLAMQFGIRAANYPLIPEDFRGTQLPSQLRPLRGKLYGLTIRPERLQEIRNER
                     RPGSRYATLANCEFEVREAEALMRQEGIPFLDATSKSVEELATTILQEAKLVRRIY"
     gene            complement(37849..38655)
                     /locus_tag="AC16CX_10775"
     CDS             complement(37849..38655)
                     /locus_tag="AC16CX_10775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3013027.1"
                     /translation="MKRIDSAANPRFKALRRLVESSSERRRAGRSVLDGVHLVAAYQE
                     HVGRPEQIAVSRSGMERPGIRALLDAMKGVDVVVMGDALFRQLAPVVTPTGILAVVET
                     PRPQPVPLEMDTCVMLEDLQDPGNLGSILRTSAAAGVRHVLLSRGSVHAWSPRVLRAA
                     MGAHFALAIYEGVDLQGVARAYPGKRVATQQRSPRSVFDADLTGSIALLLGNEGAGLS
                     AGLLSAADTVVSIPMPGAAESLNVAAAAAVCLFERVRQLRAVQSDGCRVG"
     gene            complement(38652..39098)
                     /locus_tag="AC16CX_10780"
     CDS             complement(38652..39098)
                     /locus_tag="AC16CX_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR3013028.1"
                     /translation="MKIAVMLHLLGAVVWIGGMFFAYLCLRPAASGLEPPLRLLLWAA
                     TLERFFQWVWVSIALILGSGFHMVAAVSELGRVPLNVHLMLYVGATMTLIFAYVFFSP
                     YPTLRHAVTARDWKAGAVALNQIRIAVAVNLALGLLNIVVATAGAM"
     gene            complement(39095..39673)
                     /gene="rnhB"
                     /locus_tag="AC16CX_10785"
     CDS             complement(39095..39673)
                     /gene="rnhB"
                     /locus_tag="AC16CX_10785"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR3013029.1"
                     /translation="MTLVCGVDEAGRGPLAGPVFAAAVILDRQCVIEGLADSKKLTAR
                     QREALAPEIRMRSAAWAVASASVEEIDALNILQASLLAMRRAVEALAVTPRRVLVDGL
                     HRPRLDVPVQAVVRGDATVAAIAAASILAKVARDDAMRALHQRHPQYGFDRHKGYPTR
                     LHLEALREHGVSVAHRRSFAPVRALLDKGTGA"
     gene            complement(39670..40833)
                     /gene="lpxB"
                     /locus_tag="AC16CX_10790"
     CDS             complement(39670..40833)
                     /gene="lpxB"
                     /locus_tag="AC16CX_10790"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR3013030.1"
                     /translation="MAEAPVTIGVVAGEASGDQLGVHLIRALRARLPHARFTGIGGPR
                     MQSAGMEVLYPMEKLAVRGYVEVLRHYFEILGIRRRLAARFLGQPPALFIGVDAPDFN
                     LGLELRLRAAGIPTAHLVSPQIWAWRARRMGTIKRAVDRMLVVFPFEQALYEQAGIPV
                     TYVGHPLAEMLSRVSNRAAAREQLRLPAAAKIIALLPGSRMSELEQMADLFIETAIQI
                     AAGLPDAQFLVPLVNRPTRDRFEAELYRRQRDGLNVTVLFGHAHEAMSAADVVLAASG
                     TATLEAALLGRPVVITYRMPRLSWWIMNNRRYQPYVGLPNIVAGEFVVPEFLQDEATP
                     ENLSQAVLNLMFDSAVRVRLEARFAAMAQALRQDSAGRIASALIPLIRNGIAP"
     gene            complement(40845..41618)
                     /gene="lpxA"
                     /locus_tag="AC16CX_10795"
     CDS             complement(40845..41618)
                     /gene="lpxA"
                     /locus_tag="AC16CX_10795"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR3013031.1"
                     /translation="MKIHSTAVVDPKARIASDIEIGPYAVIGPHVEIGPGCWIGPHAV
                     ITGHTCLGANNRVFQFASLGEAPQDKKYAGEATRLEIGNDNTIREFCTLNCGTAQDAG
                     VTRLGDDNWIMAYVHLAHDCQVGSHVVMANGTQLGGHVHVGEFAILGGLTAVHQYCHI
                     GAHSITGAGTVVLQDVPPFITVSGNPARPFGINAEGLKRRAFSAETITRLKRAYRTLY
                     RSGLTLDQARRELARQLKQCPEIQVVLDFLADSTRGIVR"
     gene            complement(41654..42109)
                     /gene="fabZ"
                     /locus_tag="AC16CX_10800"
     CDS             complement(41654..42109)
                     /gene="fabZ"
                     /locus_tag="AC16CX_10800"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3013032.1"
                     /translation="MSIMDINQIFKYLPHRYPFLLVDRVLSCEPGKKIVAVKNVTINE
                     PFFQGHFPNYPVMPGVLIIESMAQAAAILTFHSENAKPDERSVYFFVGIDHARFKKPV
                     VPGDTLRLEVTITRHVRGLWKFSAHAWVGEIMVAESEIMCTVRSIKGGE"
     gene            complement(42106..43158)
                     /gene="lpxD"
                     /locus_tag="AC16CX_10805"
     CDS             complement(42106..43158)
                     /gene="lpxD"
                     /locus_tag="AC16CX_10805"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR3013033.1"
                     /translation="MTLSERKSYSLREITARFGGEIAGDSGVEVRRVATLESATPESI
                     AFLTNERYLPQLRTTRAGAVIVGQSARDATRLPRIVCANPYAYFARVSALFNPARPPR
                     PGVHASAVVDSSAKIAEDTEIGPCVVVGRNARIGAGCVAGAGCCIGDDVTIGSGSTLH
                     ANVTIYHDCVIGERVILHSGVVIGADGFGIALEEDRWVKVPQIGRVVIGNDVEIGANT
                     TVDRGALDDTVIEEGVKLDNLIQIGHNVRIGAHTAIAGCAGVAGSAKIGRYCRIGGAS
                     GIAGHLVIADHVEISAYTLIAKSIDRPGTYTGAYPFEPHPQWRRNAAQLRHLYELTQR
                     IRALEGKPKKRKRSRS"
     gene            complement(43229..43732)
                     /locus_tag="AC16CX_10810"
     CDS             complement(43229..43732)
                     /locus_tag="AC16CX_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR3013034.1"
                     /translation="MKSLYATSAALMLGFAAATVGAAEIKIGYVDAERINRESAPAER
                     AARQIEKEFAPRQQELQRREAQIKALQGQLEKEAMTMSESERRAKEQEIARQGVEYQR
                     MLREYREDLNIRRNQEISSLLERANRVIRQIAEAEKFDLIVQEAVYRSPQIDITDKVL
                     KALADVK"
     gene            complement(43785..46052)
                     /gene="bamA"
                     /locus_tag="AC16CX_10815"
     CDS             complement(43785..46052)
                     /gene="bamA"
                     /locus_tag="AC16CX_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HIR3013035.1"
                     /translation="MRWFRAAWLLLALHAAAATAIEPFVIKDIRVEGIQRTEAGTVFS
                     YLPVKVGDTLTEEKAAQALRALFATGFFRDVSLEREGDVLVVVVQERPSIAQVDFTGM
                     HEFNKEQVITGFRQVGLAESRIFDKGLLDRAEQELKRQYLGRGYYAVNVSTTVTPLER
                     NRVSINFNVEEGRVAKIRQISIIGAQTFRESELLGQFVLRTPGWLTWFSKHDQYSRQR
                     LSADLESLRSFYLDRGYLEFRIDSTQVSITPDKQDIYLTISITEGSKYTVSDIKVAGE
                     MLIPEDEVRKLIQIKPGEVFSRARLTESTKLITDRLGNDGYAFANVNAVPELNREKQQ
                     AAFTFFIDPGRRVYVRRINISGNTRSRDEVIRREMRQLEGGWYSAERITLSKQRIDKL
                     GYFTEVNLETPAVPGTTDQVDVNISVVEKPTGLLLFGAGYGSGEGLILSGSISQDNIF
                     GSGKHVSASINSGKINTVYSLSYTDPYFTVDGISQGFDVYYRLVTPSDFGLARYETKT
                     LGGQLRLGVPVSEIDTIHYGLGYENVDITIFPDSPLIYKDYVTTFGPSNSNLFATAGW
                     TRDGRDSLIYPTKGTLHKAGIESGLPGGDLQYYKLSYQYQRYFPLTRHLTFLLNGEGG
                     HGDGYGAKPLPFFKNFFAGGVSSVRGFRSYTIGPKDSNGNPRGGSSKVVGNAELFFPF
                     PGLENDRSVRLSTFFDTAVVGDKIDSNELRYSAGLSLLWVSPLGPLKVSVAKPLRKQP
                     GDSTQAFQFTIGGVF"
     gene            complement(46057..47427)
                     /gene="rseP"
                     /locus_tag="AC16CX_10820"
     CDS             complement(46057..47427)
                     /gene="rseP"
                     /locus_tag="AC16CX_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR3013036.1"
                     /translation="MTLLTTLIAFAAALGCLIVVHEFGHYLVARACGVKVLRFSVGFG
                     KPLWARSLGRDGTEWVIAAFPLGGYVKMLDEREGQVAHEDLPRAFNRQSVWRRLAIVV
                     AGPAANFLLAIALYWGLFVHGVPGIRPVIDEPPAGSAARLVGFAAGDTLVRIGDEPVA
                     TWQDARWILLQGAVQKSVVTIEVRGAGGSIVWRKLDLSGLTPADLDSDFLRTLGLMRH
                     QPKLKPVIGDVVSGGAAHRSGLKAGDEIVAIGNRRIMTWDQVVNIVRSEPGAPLVVEV
                     RRDGVLQPAIAVTPEVVIENGRKIGRIGAAPRVDRSALAGMIVEVRYGTIEALAKALH
                     KTWDTSVFSLMMLGKMIVGDVSLKNLSGPITIADYAGQSAQSGVASYLLFLALISISL
                     GVLNLLPIPLLDGGHLMYYIVEIFKGHPISERAIEIGQHVGMALLFTLMAFALYNDIT
                     RLLVSG"
     gene            complement(47464..48690)
                     /gene="ispC"
                     /locus_tag="AC16CX_10825"
     CDS             complement(47464..48690)
                     /gene="ispC"
                     /locus_tag="AC16CX_10825"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3013037.1"
                     /translation="MNDKQRLTILGSTGSVGVNTLDVVARHRNRFDVVALTARNQAET
                     LFKQCLEFRPRTAVVLEAAAAQDLRQRVRQAGLDCEVRSGVEALEEVAGFPDVDTVMA
                     AIVGIAGLRPTLAAARSGKKVLLANKESLVTAGAVLMDAVHASRATLLPIDSEHNAIF
                     QALPRGYAAPDAAGVRRILLTASGGPFRSLPLRQLDAVTPDQACAHPNWAMGRKISVD
                     SATMMNKGLEVIEARWLFNVDPENIEVVIHPESVVHSLVEYIDGSVMAQLGYPDMRTP
                     IAFALGFPERIAAGVEYLDLARVGALHFERPDIQRFPCLRLAYEALKAGGSAPTVLNA
                     ANEIAVASFLSGALGFRRIPALIETVLSKVPCGPVTGLEDVFAADRLAREQAEVWLHA
                     SRSAGAAAHLRQTVRS"
     gene            complement(48704..49522)
                     /locus_tag="AC16CX_10830"
     CDS             complement(48704..49522)
                     /locus_tag="AC16CX_10830"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3013038.1"
                     /translation="MFTARLATAAVLLAVCISALLFLPNRWWAVLLLPVLLAASWEWG
                     ALAGLRRAPRWIFAGLVSVSAVAVWLLVTGAAPYTGRLTVAEALIYGSGCVFWLLIAL
                     PWLAQRWRLRSPLALCAAGWIVLVPAWLALARLQSDPEQLLAVLGVVWVADTAAYLSG
                     RAWGKHPLAPHISPGKTWEGLAGAGAAVAVYYVVLSSAAAGWGWWHGTGGVVLFAGVG
                     LMSVVGDLYESWVKRLAGVKDSGTLLPGHGGILDRIDSLTAAMPVAALLLPYAG"
     gene            complement(49558..50355)
                     /gene="uppS"
                     /locus_tag="AC16CX_10835"
     CDS             complement(49558..50355)
                     /gene="uppS"
                     /locus_tag="AC16CX_10835"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR3013039.1"
                     /translation="MKTFASSTLEIPDARRIPRHIAIIMDGNGRWAKQRFLPRVAGHR
                     RGLDAVRTVVRACVERGVEFLTLFAFSSENWRRPAEEVSFLMQLFAMALEQEVGKLHE
                     NGVRFKAIGDLGRFEPKLRQLVREAEALTAGNQRLTLTIAANYGGRWDVVQAVSRMLR
                     ERPELASGFGERDLAPYFALCYAPEPDLFIRTGGEQRISNFVLWQLAYTELYFTDILW
                     PDFDAAALDRAIVSYQQRERRFGRTSEQLPADAGLAAFRVENNRSAD"
     gene            complement(50355..50912)
                     /gene="frr"
                     /locus_tag="AC16CX_10840"
     CDS             complement(50355..50912)
                     /gene="frr"
                     /locus_tag="AC16CX_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3013040.1"
                     /translation="MIADVKKTAEQKMKKTVETLKSDLAKVRTGRAHAGLLDHIMVDY
                     YGTPTPIPQVANVTLLDARTIGVNPWDKKMTNVIEKAIRDSDLGLNPATSGETVRVPM
                     PPLTEERRKELTKVVRHEGENARVAVRNVRRDAIHHLKELLKQKKVAEDEERRAQDEI
                     QKLTDRHIVEIDKLLQQKEADLMAI"
     gene            complement(50939..51658)
                     /gene="pyrH"
                     /locus_tag="AC16CX_10845"
     CDS             complement(50939..51658)
                     /gene="pyrH"
                     /locus_tag="AC16CX_10845"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3013041.1"
                     /translation="MPQTPAYKRILLKLSGEALMGEDSYGINRETVDRIVGEIAEVVN
                     LGVEVAVVIGGGNIFRGVAPASSHMDRATADYMGMLATVMNALALQDAMRRVSLVSRV
                     QSALNIEQVVEPYIRGKAMRYLEEGKVVIFAAGTGNPFFTTDTAAALRGMEMNVDLVL
                     KATKVDGVYTDDPKAHADAMRYKRLTFDEAIIKNLKVMDATALTLCRDQKLPINVFSI
                     FKPAALKRVVMGEDEGTFLHN"
     gene            complement(51702..52586)
                     /gene="tsf"
                     /locus_tag="AC16CX_10850"
     CDS             complement(51702..52586)
                     /gene="tsf"
                     /locus_tag="AC16CX_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3013042.1"
                     /translation="MAEITAGMVKELREKTDAPMMECKKALTEANGDMGKAEEILRVK
                     LGNRASRAASRVTAEGAVAAYIAPDGKSGALVEVNCETDFVARNGEFLGFAGALAELA
                     ATQRPADVGALSRLPYRGATVEEARKALVGKIGENVSIRRFVRMTAQGKLATYIHGGA
                     RIGVLVDVAGGGDTLARDLAMHIAASKPVALSAATVPAELVQKERSIAAAKAAGSGKP
                     PNIVEKMVEGAVQKFLKEVTLLGQPFVKNDKQSIEQLLKSEGASVSGFALYVVGEGLE
                     KRKDDFVAEVMSQVSQAV"
     gene            complement(52650..53384)
                     /gene="rpsB"
                     /locus_tag="AC16CX_10855"
     CDS             complement(52650..53384)
                     /gene="rpsB"
                     /locus_tag="AC16CX_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3013043.1"
                     /translation="MSVTMREMLEAGVHFGHQTRYWNPRMAPYIFGHRNKIHIVNLEK
                     TLAMYQEALKFVRQLAANKGTILLVGTKRQAREIIREEASRCGSPYVDYRWLGGMLTN
                     YKTVKQSIKRLKDMEAMMQDGSIEKLPKKEALHYQREIAKLDRSLGGIKDMNGLPDAL
                     FVVDVGYQKGAVAEANKLGIPVIGVVDTNHTPEGVAYVIPGNDDSSRAIRLYVRGIAD
                     AVLEGRQDSLKEIVRGGDEFVEVEEA"
     gene            53665..54465
                     /gene="map"
                     /locus_tag="AC16CX_10860"
     CDS             53665..54465
                     /gene="map"
                     /locus_tag="AC16CX_10860"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3013044.1"
                     /translation="MPVSIKSPQEIEKMRIAGRLAAEVLDFIAPHVRSGTTTGELDRL
                     CHEFISQELKATPAPLNYAPPGYQPFPKSICTSVNHVVCHGVPGDKKLRTGDIINIDI
                     TVIKDGFHGDTSRMFYVGHPSIQARRLCEVTYECLWRGIESVRPGDHLGDIGHAIQNH
                     AESHGYSVVREFCGHGIGRKFHEEPQVLHYGRPGVGLKLEPGMTFTIEPMINAGKPDV
                     RQLADGWTVVTKDHSLSAQWEHTVLVTESGHEVLTLSAGSPAKPALNA"
     gene            54469..57063
                     /locus_tag="AC16CX_10865"
     CDS             54469..57063
                     /locus_tag="AC16CX_10865"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HIR3013045.1"
                     /translation="MAGLQAVTAAAPALQARVGQWKQKLAALRLQLRDQFFIHQSPKD
                     LLRRQSTTVDRQLKEMWASHAMPRNVALAAVGGYGRGQLFPCSDVDLLILLPATTDAA
                     LARKIEDLIGTLWDIGLEVGHSVRTLDECVALAATDVTVQTTLLEARLLAGKRDLFAR
                     FVKRMRDLLDPAEFMQAKLLEQQQRHVRYAATTLEPNIKESAGGLRDLQTILWIARAA
                     GIGKSWRELAHRGVITSAEAREINQHEQFLQSLRIRLHYRAGRREDRLLFDFQTALAH
                     EIGLHDRPHRLASEQLMQRYHRVAKAVSQLNAIVLQNLDARIVPPRDNEYRPINGRFG
                     IRDELLEARDEQLFERHPSAILESFLLLQQHHELKGMSAATLRALWRARRLINPAFRR
                     NPANREQFLNVLKSPSHVERALRRMNENDVLGRYLPTFGRIVGQMQHDLYHVHTVDEH
                     ILRVIRNLRRFSIPELAHEFPLCSRLMSGFARPEVLYVAALFHDIAKGRGGDHSLLGT
                     VDARRFCREHGLAREDVELAAWLVENHLVMSITAQKQDIADPAVVQAFARRVGDERHL
                     IALYLLTVADIRGTSPKVWNAWKAKLLEDLFWLTRRLLAGGDRPTLASSLQARQDEAR
                     AKLRLYAVPADAENKLWAQLDDAYFLRHEPQEIAWHTRLLYYRVQSHGPVVKARLSPA
                     GEGLQVMIYVPDQKELFARICSFFERISYDIFEAKIYTTRHGYALDSFQVQDPDNRRP
                     QYRDVIGLIEHDLAERLQHKTPLPPLSKPRLSRQLRHFPISPEVNIQPDEKGTSWVLS
                     VIAGDRPGLLSRIARVLTAYDVNLNTAKINTLGARAEDVFIIAGEALKDPKTVVRLES
                     DLVEQLRT"
     gene            complement(57148..57681)
                     /gene="def"
                     /locus_tag="AC16CX_10870"
     CDS             complement(57148..57681)
                     /gene="def"
                     /locus_tag="AC16CX_10870"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3013046.1"
                     /translation="MTVRTVLRMGDPRLLEPSRPVDRFNTPELHALIADMDDTMRALD
                     GAGLAAPQIGMGLQVAIFGVEHNPRYPDVEPVPYTVLINPVLMPVGNEMEEGWEGCLS
                     VPGMRGLVPRYRSLRYQGYDQYGGAIDRTVSGFHARVVQHECDHLAGILYPMRIRDLR
                     NFGFNEELFPGQDLQDE"
     gene            complement(57671..58417)
                     /locus_tag="AC16CX_10875"
     CDS             complement(57671..58417)
                     /locus_tag="AC16CX_10875"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HIR3013047.1"
                     /translation="MLAVIGGSGLTQLANLEVTRRLVVRTPYGEPSGALTFGTIGGRE
                     VVFLARHGYGHTIPPHEVNYRANIWALHAQNVKDVVSIASVGGIRADLGPGTLAVPDQ
                     VIDYTHGRNSTFFTRTDRAVTHIDFTHPYCEAMRQRLLKATAAASEPAIAGGTYAATQ
                     GPRLETAAEINRLERDGADMVGMTGMPEAALAREIGLCYAAIAVVVNYAAGRGTSTQG
                     VRLADINEVSQRAMARVRNILDRLAALDDG"
     gene            complement(58492..59064)
                     /locus_tag="AC16CX_10880"
     CDS             complement(58492..59064)
                     /locus_tag="AC16CX_10880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HIR3013048.1"
                     /translation="MALSREQALRVLDTADLLCSAADVSGAVARMAREITAALAERFP
                     LVLCVMRGSVVFAGQLLPQLRFPLEFDYLELTRYREATHGGEIEWKVTPGTSVAGRVV
                     LIVDDILDEGHTLAAIRDKLVGAGANRVLSAVLAEKNTGRAKPVSADFIGVRLPDRYV
                     FGFGMDVSGAWRNLPEIYALKEDGVVRIED"
     gene            complement(59065..59433)
                     /locus_tag="AC16CX_10885"
     CDS             complement(59065..59433)
                     /locus_tag="AC16CX_10885"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3013049.1"
                     /translation="MADKLMIVMVNTDPSNTAELGAPFFQATVAAAMDYEVEVILTAR
                     AGELAKKGVAEKLYVKEGSGKSVYDFIKDAHQAGVHFKVCTPALDLWGNDLIPEIEET
                     VGGAYVIQQAMDDRVVTFTY"
     gene            complement(59578..60459)
                     /gene="galU"
                     /locus_tag="AC16CX_10890"
     CDS             complement(59578..60459)
                     /gene="galU"
                     /locus_tag="AC16CX_10890"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR3013050.1"
                     /translation="MNRIIKAVFPVAGMGTRFLPATKASPKEMMPIVDKPLIQYAVEE
                     AMAAGMTEMIFVTGRGKRAIEDHFDKAFELETELESRGQKQLLKLVQGILPRRVSCTY
                     VRQPQALGLGHAILCTRPVVGDAPFAVVLADDLIDGTPPVLKQMCAVFARRGRSVIAV
                     QNVERHETRRYGIVKLDPRSRSPHRISAIVEKPEPAAAPSTLGVVGRYLLTPRVFHHL
                     QRVRPGAGGEIQLTDAIAALLQEEDVFAHEFAGTRYDCGSKLGYLQANLQYALRHPEV
                     GMEFRRYLRGLGRRLPK"
     gene            complement(60463..62490)
                     /gene="ligA"
                     /locus_tag="AC16CX_10895"
     CDS             complement(60463..62490)
                     /gene="ligA"
                     /locus_tag="AC16CX_10895"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR3013051.1"
                     /translation="MAVPKPVTARAARLRDEIERANYSYYVLDAPTLPDAEYDRLFRE
                     LQDLESRHPALATPDSPTLRVGTAPLTAFAQVTHATPMLSLNNALSIEEVEAFDRRVR
                     EALDLEGEVEYAVEPKFDGLAVSLTYERGVFAMGATRGDGYTGEDVTANLRTVRALPL
                     RLRGESVPGLLEVRGEVMMLKRDFEALNREQRGKGEREFANPRNAAAGSLRQLDSRVT
                     ASRRLTFFAYGLGRFTGRRVPRDRHSRQLDYLEALGFPVSRERRVVHGVKKLLGYYHE
                     VERHRAGLPFQIDGVVYKVDDLSQQDRLGYVSRAPRFAVAHKFPAEEEITQIVGIDVQ
                     VGRTGALTPVARLAPVAVGGVTVTNATLHNEDEVRRKDVHIGDYVVVRRAGDVIPEVV
                     GVVRERRPKAVRVFHMPAQCPVCGSRVERIEDEAIARCTAGLYCPAQRKQALLHFASR
                     RAMDIEGLGEKLVDQLVDQGVVENPAGLYRLDPGTLAGLEHMGEKSASNLLAAIAKSR
                     DTTLARFIFALGIRNVGEATARDLAQHFGGLDALIEADEAALQRVTDVGPVVAASIAR
                     FFREPHNREVVKQLRKHGVRWPEGKGGGAASLPLTGKTFVLTGTLPHLAREEAKEKIE
                     ARGGKVAGSVSEKTDYVVVGTDPGSKHDKARALGIALLDEDGLLKLLGEKA"
     gene            complement(62493..63569)
                     /locus_tag="AC16CX_10900"
     CDS             complement(62493..63569)
                     /locus_tag="AC16CX_10900"
                     /inference="COORDINATES: protein motif:HMM:NF016257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HIR3013052.1"
                     /translation="MSDLQLSLLVIGAVVVAAVYLYNWLQERSFHRRMREAFGAAHDD
                     VLLRAGVESTLADGRLEPQLAPQEPARAEGGRSAVRPSRDADSVAGFDDELDYVAEID
                     AGTPVSDTLIGELMSKTASCGKPVRIAGLALQNEQWEDVVRGSGGRYRKLRLSLQLVS
                     RSGIVNSAQLAAFCDAVRSCADKMPAVCPDAQAALKRARELDAFCAGVDVAVGVNVVA
                     TEGATFPGTRIRALAEAAGFKLEPDGVFRYRDEQRQTLFTLDNHEPAPFLPESIKGLS
                     TRGITLLLDVPRVASGVEVLDRMLEIANRFAAALGGRLVDDNRVALSEAGIERIKEQV
                     RSIHATMSARGIPAGSNRAQRLFS"
     gene            complement(63579..>65491)
                     /locus_tag="AC16CX_10905"
     CDS             complement(63579..>65491)
                     /locus_tag="AC16CX_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR3013053.1"
                     /translation="LEAVLHQRLNAIALERMEQSGRWFNEPPPGRITVFAPAREAAAP
                     ASPLAGCEPLRRYVTCNDARLEAVIGDWLHQVYMITDAATGLALRERLPAGAMLVTRE
                     GHIFTRHTASFHAPDSELHGVLSRQREIEQLDGAIGTARAELDRLREATAAVAKEVEQ
                     GRARLEELRDETNDRQQRHHALQLEALQLAELDHRITQRTERIASELVEIEAESAAAT
                     SNGEAAARRSAELQSEVSGLREQLTLATDLYRRAENVLELQRNALQQAREAAQQAVFH
                     QQSCNNKIIDITNSIKVISEGEQRLGTALDSRRKEFASYDEAPLQQRLQQALAARSER
                     EQALARARDALEGMEGSLKATDEERHTAEQKLEPLRERIADMRLKEQEARLNEEQYTQ
                     QLAEAGAREEEVAQQLEKGMRPGALQGEITRLNEDIKSLGAVNLAALEELQAARSRKE
                     YLDAQSRDLNEAMATLEDAIRRIDRETRERLQHTFDEVNRHFGQLFPALFGGGHARLV
                     LTGEEILDAGVQVTAQPPGKKNTTIHLLSGGEKALTALALVFSIFQLNPAPFCLLDEV
                     DAPLDDNNTERFCELVKKMSQQSQFLFISHNKITMEIAEQLLGITMQEPGVSRVVAVD
                     IEEAMKLREAAA"
BASE COUNT        11342 a        21701 c        20453 g        11995 t
ORIGIN      
        1 gattcttgat gacgtccgcc agaaggtcat ccggccgtac tttggcgagc acaccggcat
       61 ggcggccgaa cggggtgcgc acgccttcgt agagataggc atcgagcatg gcaatctcct
      121 ttcaggaaat cgttgcggtt gcggtcatca gggttccggg gtctgcagcg acacgcccaa
      181 ccgggcgcgc cgcgtgagcc agatggtagg acggtaacgc ggatcgccat agacacgata
      241 catcccggac agcaccttgt atacgcgatc cgggccgagc acgtcgccga atttgaacgg
      301 gccgttcgga taattgagac ccaacgccac ggccttgtcg atatctgcgg gctgggccgt
      361 gcggctctgc gcgatggagc aaccgatgtt gacgatcatc gcgacgatgc gctgcgcgat
      421 gaagcccggg ctgtcgcggc atactgtcac cgggatgccg tccgcggcga gcagcccgtg
      481 cgcgccctgg cgatagacgt cgccggtcag cggcgtgccc atgagggtgc ggcgcttggc
      541 catcgggaac agcgtatcca ccgctaccgt gcgcttcgga tcgagcccct gctccaccgc
      601 gcaggtagtt gcgtcctcgc cgatcggcgt caccaggatg agtgcgtcag cggccggctt
      661 ggggccggtc tcaagtcgcg cgcccagctt cttgagcagc tcggtcatca cggcatgccc
      721 tttcggttcg gccgggctca cccatacgcc gccgcggtaa gcggcaggcg cgggcggctc
      781 cggcgcgaga accggcttca tttccgcgtc gtactcgtag aagccgcgcc tggtcttgcg
      841 ccccagcacg cccgcctcgt agcggctgcg catgatgaga ttggggcgat agcgcggctc
      901 ctggaagaac tggttgtaga tgagctcgga tgcggggtgg gtgacgtcaa gcccggttag
      961 ttccatcagc tcgaatggcc ccatgcggaa cccacccacg tcccgcatca cgcggtccac
     1021 gtccacaaag ctcgcgatgc cttcatatac caagtgcgag gcctccagcg taaagccccg
     1081 tcccacttgg ttgactagga agccgggcgc atccttcagc cgcaccggct cgcgcgtcat
     1141 gcggcggcct atcgtcatca gcgcctctgc gacccagccc tcggtccgca atccctcgat
     1201 cacctctacc agcttcatca gcggcaccgg gttgaagaag tgaaagccgg ctaaccgtcc
     1261 gggcgtcttc agcttcgagg cgatggtggt gacggagagc gaggaggtat tcgtcgccag
     1321 cacgcaatcc ggagcgacga ccgattccag ttcggcaaac agctcctgct tggcctggag
     1381 gttttcgacg atggcctcga tcaccatgtg acacggcttc atgtccgctg gcccgccgac
     1441 gatctggacg cggtccaccg ccgcgaccgc ctcatcccgg gtgatcgtgc ctttttccgc
     1501 ggcgcgcgcc agcatcttgt ccacgaaatc gcgcgcctca tcggctgcgc ccggccggct
     1561 gtcggtcatc aatacccgca tgccgcccgc ggcggcgacc tgcacgatgc cccgccccat
     1621 tgcccccgtg ccgatcacgc ccacaatgag atcggggcgg cttgcgtcga gtgccatgat
     1681 ttcccctgta gatgttcctg gaacggcgcc gctcagcgac ccttgaattg cggcttgcgc
     1741 ttgtccatga atgcagcctg cccttccttg tagtcctcgc tctcgaagca ggcgtcaacc
     1801 atgcgctgac actgcgccag gtcccgttgc ggagcgtccc tttgcagctc gatcaacgaa
     1861 cgcttcaccg ccgcgatggt gagcggcgcg ttgacggtga tggtgttgat gtagtcgagt
     1921 gtatagctct ccagttcgcc tgtaggcacg acatgattca ccaggcccat ctgcagtgcc
     1981 tcctgcgcgg tgaactggcg cgccgtgaag aagatttcgg cggtgaacga agggccgaca
     2041 atactggcca agcgcctgat gccctcgaac cggtatccga gcccgagctt ggcggcgggc
     2101 acgccgaagc gcgcgtcgtc cgcggcgatg cggatgtcgc aacccaccgc gacggcggtg
     2161 ccgccaccga tgcaataacc gcggatcatc gcgatcgttg gcttggcgca ttcccgcagc
     2221 gcccggttgg cggcgtcggc tgcagcgttg tattccttca ccgcttccgc gctggtgcgc
     2281 ttctccttga actgggagat atccgcgcca gcgctgaacg ccttctctcc agttcccttg
     2341 aaagcgatga cgcggatttc cgggtcgttg acgtacgtct ccagcacgac cggcagcgac
     2401 tgccacatct cgtaagaggt tgcattgtga cgttccgggt tgttgaagat gacccagccg
     2461 atcgcccctt ctcgtttggc gatcagcttg tccgtgaggg tctgaatcgg cgcagccgga
     2521 tgattcatgg ctgtatgtat cctgtatgct ggcgaggccg gtagtgtagc cgattcgggg
     2581 cgcccattga ggggcgggag accacgttgt gatagatttg agcaaggcat cctgaaaaac
     2641 tacggagagg gaccatgcga cggttggtta tggcatgcgc tgccatcatc ctgggcctgg
     2701 cttgtttcgc cgcgccggcg cagggctatc cctccaaacc gatccgtatc atcgtcacct
     2761 tccctcctgg cggtcccagc gatttcgtgg cccgggccct ggcacagaaa ttgaccgaga
     2821 catgggggca acaggtcgtc gtcgataatc gcagtggcgc tggcggtgtt gtcggagtgg
     2881 aagccgccgc gcgctccagc ccggacggct ataccctgct gcaaggccag tcgggtggta
     2941 tgagcatcaa tcccgccttg cacgcgaagt tgtcatacga cccgtttcgc gatttcatgc
     3001 ccgtcagcat gctggtgatc aatgcgcagc tgctcgtggc gcacccctcg ctgccggtca
     3061 gatcggtgaa ggacctcgtt gcgctggcaa gacatcggcc gggacagatc agctacgcat
     3121 cggccggcta tggcagcgcc cagcatctgg gcatggaaat gctgaaagtg atgacaggca
     3181 ttgacctgtt gcacgttcca tacaagggca cagggcccgc gctgattgac ctgttctcgg
     3241 ggcaagtttc gctgcaattt accagcatgc ccgcggtgct gcctcacgtg aaggccggca
     3301 agctcagggg cattgcagtg ggcagttcca agcgctccgc ggcagcgccg gacattccca
     3361 ccgtctcgga atcaggagtg tccggtttcg agtacgtagc gtggtacggt ctgttcgcgc
     3421 ccgccggaac tccgcaagtc cttgtcaatc agatcaatgt ccacgtcgtc aatgctctcg
     3481 gtaatgtgga actggcacag cgcctggcca gccagggagc agagccctcg cccagcactc
     3541 cggaggggct tgccaagtac ctgcgcgaag atcacgagcg ctggaagaaa gtcatccgaa
     3601 gcgccaacat tcgcatcgag tgatcgccgc gttggcacgc ggcgatcaac gcgtcgacga
     3661 ttccccgtca gcgcacgacc agcacgggga ttttcgagtg ggtgagcacc ttggcggttt
     3721 cgctgccgag caggaggctc gacaggccgt ggcgaccgtg agaagccatc atgatgagat
     3781 cgcacttctt tttccgcgcg gtcgcgatga tctgctgata gggcaagtcg cttgccacac
     3841 tcacggtcgc gcaccgcacg ccccgggcgc gtgcctgccg cgcaatccca tcgagcattt
     3901 tctgcgcccg cgtccgcgcc tcatcctcaa atcgtttctt caaggcggaa ttgaatatcg
     3961 tgacgccttc atccaccatg agcttgaact cgggcacgac gtgaatcgcg gtcacgcgcg
     4021 ccctgctttg cttcgcgaac tcgatgccgc gcttgaccgc cttgttggac atcgttgagc
     4081 cgtcagtggg taacaatatg tgcttgaaca tgatgcctcc gtccgactct ggaactacgg
     4141 acctcaacga agattaccat gctatcactg agggggactg atctaggtca accgcctccc
     4201 gcccgtgccg ctgcggggag tcaaaaatag gatcggcggc aggaggccgt gccgctacgc
     4261 agtcagtgcg tcttggccag acgatgggcg ggaatgggaa gggccacgac ttcgatcccc
     4321 tcctccttca gggactcgac ctcctcgcgc gatgccgtgc cgcggatccg acgctcgggc
     4381 gcctcgcggt agtggatttt gcgcgcttcc tcagggaaag cggcgccgac gtcatcggta
     4441 ttctcgatga tacgctcgat gagcttggcc agcgcctcac tgcccacgtt ggcatactgt
     4501 tgctgagtgc cggcagccgg ctcggttcgc gccgagcgtt ccttgccgcc ggtcttgacg
     4561 tgggaggcat gcggcaggcg cacgacattg gcattgccgc acaacggaca gctgatgagc
     4621 ttgcgtccga tctgacgctc gaaatcctcg ttcgaggcga accaaccctc gaagcggtgg
     4681 ctgttctcgc agcctaaatt aaaaacgatc ataatgctcc taaactcacc ccgaaatggt
     4741 gggcgagacc ggattcgaac cggtatggct ttcgccgagg cattttaagt gccttgtgta
     4801 tgccaattcc accactcgcc cgtcatccta tccctttgcg gaccttgaca gccagcttct
     4861 aacggtatct gacgagacgc tcatttccct cgcgtatgct gccatgctgc cgtaacgctt
     4921 gattccttcc tgtacatctt ccggagcggg gatatgcgga tatttcgtgt tcttgccccg
     4981 atacgttggc gtaagcgcgt gacaattggg gcagatcaac cttatgttgg cgagctggtt
     5041 attggtcttg tcgccatcaa catgatccag ttccagcggg atcgatctgc ccagccattc
     5101 aaccccgcca caactcgagc atttcggctc tagcacacct tcacgcaaaa gcctttttct
     5161 cagtttgttg ctttgatagt aggtgccgcg acgcaagatg tcttgcaagg gataccggcg
     5221 gatatgcgga ttggtcttgc ctcgcagatg cgcctgcccc gtccagtgtt cggtggcaag
     5281 ccgcaacgcg ttgatccgca tttttaccaa ctcgtagttg ccgccggccg cacgcagtcc
     5341 caatcgacgt aagacctgcg ccatgctggt tgattccgca acggcagcga ccagattcgt
     5401 atcggtccag gtcctgcgtc tgccccgcat atcggtgtct gctgtccctt cgaaaacaaa
     5461 attatagcag acatgccgga tagctatccg aagtctcttc agaatatcca gctgctttca
     5521 tggcagcagc tggcgattgg aggcgggggt cggaatcgaa ccggcgtcca cggctttgca
     5581 ggccgctgca tgaccactct gccaccccgc cgcggataaa aaaagcaaaa ggggaaaacg
     5641 gcaatccgtt tcagcggggg ctaaccttta ggttatgccg gaactaaaac gacctggtgc
     5701 cggtttcccc ggaaaacctg gagcgggaaa cgagtctcga actcgcgacc tcaaccttgg
     5761 caaggttgcg ctctaccaac tgagctattc ccgcatttcg taagaaaaat tataggacag
     5821 ggcccccttc cgggcaacta tggccgctgc catgcctgcg gcagggcgac cttgagatag
     5881 tacgccatcg agaccagcgt cagcagcgcc gcgagataga tcagccaggt gcctacctgc
     5941 tgcgggctga aggcaccgat gctgtcgtgg tagagcagca gtggtatcgc cagcatctgg
     6001 gaggtggtct tgatcttgcc gagaaacgat accgcgacgc tcttcgcggc cccgatctgc
     6061 gccatccatt cacgcaacgc cgagatcgcg atctcgcgcc cgatgatgat gagcgcgatg
     6121 atcgcatcca cgcgctcgag ctgcaccagg atgatcaagg ccgccgccac catcagcttg
     6181 tcggcgaccg gatcgagaaa agcgccgaag gcggacatct gattaagctt gcgcgcgagc
     6241 cagccgtcaa gccagtcggt gaccgccgcg gcggtgaaga ggatggtcgc caccaggttc
     6301 tggttgggta tcgacaccca gctcttctcg aaatagaaga tgcctacaaa aagcggtatc
     6361 aggatgatcc gcagccaggt cagcaggtta ggcaggttga acggcatcga gatcgatcag
     6421 gttgttgaaa aatgtcccgc ggatcacatt gtgaacccaa acaccaccgc aggttccctc
     6481 gcaaccaccc cctgcttcta gggacgggaa agggggcctc gctccctttt ccacatcccg
     6541 ctaatgcagt tcatgataaa tcttttcggc gagcgtgcgg ctgatgccct cgacctgagc
     6601 gagttcatcc acgccggcgg caagcagccc gcgcaggccg ccgaaacggg ccagcagccg
     6661 ctggcggcgc ttcgcgccga cccctgcgat tttctccagc gtcgagcggt tgcgcgtctt
     6721 gccgcgtcgg gcgcgatgtc cctggatggc gaaacggtgt gcctcgtcgc ggatctgctg
     6781 aatgaggtgc aggcccaggt ggcccgcatc cagcttcagc gggttcgctc gaccggtgag
     6841 gaatagctgt tcctgccccg gtcttcgatc ttcacccttt gcgaccgcca tgaccatcac
     6901 atcgctcatg ccaagctcct caaagacatc aatcgcgacg gcaagctgcc ctttgccacc
     6961 gtcgatgagc acaagatcgg gcattttgcc ctcacccgca acgatcttgc gatagcggcg
     7021 cgtcaatgct tcgcgcatgg ccgcataatc gtcgcccccc gttatggcgg cgcgatgaac
     7081 ggcgccttgc atccgttcga tcgcgccgga gtcaagcggc gatacatcgt ccgtcttgac
     7141 tcgaatgtta taacgccggt actcgttttt ctgcagcgtg gacttgtcgt acaccacgca
     7201 cacggcgaca gtggcttcgc ccatgatgtg gctcacgtcg aaacactcga tgcgtcgcgc
     7261 cgtctcgggc aactcaagcg actgctgcag cgcaaccagg cgtgcctcct gggttgccag
     7321 cagtcccagc gcctgcgcgg cgccaagctg cgcgttgcgt tccgccattt ccagccaggc
     7381 tctgcgcacg ccggccggat gctgatggat tctcaccttg cgaccggcac gctccgacag
     7441 cagttgctcg agtggccgcg catccagttt cgccccgacc acgatctgcg ccggcacgcc
     7501 gtgctgcaga tagtgctgct gcacgaatgc ctcgatgacc tgcccggcgc tgcattcatc
     7561 ggcgttgcgc ggataaaaat tcttgtcgcc gagatggcga ccgccccgga tcatcaccag
     7621 gttgacacag acggcgcctt ctgcgcgccc ccatgcaacg acgtcggcat catcgccctc
     7681 cccgctcacg aactgcttct ccctcacctt gcgcaaggcg gcgatccggt cccggaacac
     7741 cgcggcttcc tcgtaattca tgcgggcaga cacgtcgtgc atgcgcgttg ccagacgttc
     7801 gatcacctcg ttttccttgc ccgccaggaa tagttcggcg gctccgacgt cctccgcata
     7861 atcgccctca ctgatcaggc cgacgcacgg cgcggtgcag cgccggatct ggtgcaacag
     7921 gcacgggcgg gagcgattgc tgaagacggt gtcctcgcag gtgcggatgc gaaacacctt
     7981 ctgcagcagc tgtatgctct cgcgcaccgc gcccgcgttg gggaacgggc cgtagtagcg
     8041 atggcgcttg tcgagtgctc cccggtggaa gccgaggcgc gggtagcggt gccctgtgat
     8101 gaccagatac gggtaggact tatcgtcacg aaacagtatg ttatatcgag gacttaaagc
     8161 ctttatgagg ttgctttcca ggagcaaagc ctccgcctcg gaacgggtga cggtggtctc
     8221 caccgccatc acctgttcga gcatgacctg gatgcggggc ggcaggccgc tcgctttctg
     8281 aaagtaggaa gcgacccgct gtctgaggtc gagggccttg ccgatataga gcacctcccc
     8341 cacgctgttc agcatgcggt agacgccagg cagatgcggc agagtggcaa caacggtggc
     8401 gggatcgaac accacggcgg caggaaaaag agcgcggtta ccgctcccct ctcgctgtcc
     8461 ggcgacgcgg gcggcgacgg gaatggatca aatatcggct gatggcgtca aacacgctcc
     8521 ggaacatctc cggggtaaga cgcttggtct gcgtattata gcggctgcaa tgatagctgt
     8581 cgtagagcgc caatccgcgc gggagcgcat gcagggcgcc gtggcggaac cggtgtgcgg
     8641 cgcgcttgag gccgagcgcc atcagcaccg cgtcatgcgc gatcgccccc aacgcaagta
     8701 tcgccgcgcc gtccggcatc gaacggattt ctgccgctag ataggcgttg cagcggcgca
     8761 cctcgtcggg caagggccgg ttggcgggag gcaggcacct gacggcgttc gtgatgcggc
     8821 agttctcgag ccggagcccg tcggccgccc ggacggacac cggccggctg gcgaagccgt
     8881 agcggtggag cgtgtcgtag agcaggatgc cggcgaaatc ccccgtaaac ggccggccgg
     8941 tgcggttggc gccgtgcaga ccaggcgcca atccgacgat cagcagcttg gcgcgcgccg
     9001 gaccgaacgg aggaaccggg cgcgcgtgat aatccggatg ccggtcgcgt atccggtcca
     9061 gatatcgcgc cagccgcgga caacgccggc atgcggtcgt gaattcctgc atcgagtcaa
     9121 acagtgtaga attacgccct tttttcaggc aacgcagcga aatggctaaa gttctggcaa
     9181 ccgaaatccg cgccggcaac ctgatcgaat gggacaggcg cgtctggcgc gtgctcaagt
     9241 cctatcacgt tcacgtcggc ggacgcggcg gcgctttcat gcagatcgag atgaaggaca
     9301 tcgagtcggg caccaagcgc aacgagcgca tccgcaccga ggacaagatc gaacgcgcgt
     9361 tcgtcgagcc gcgtgagatg caatatctct acagcgacgc cagcggctac gtcttcatgg
     9421 acaaggagac gttcgagcag ctcacccttc ccgccgattt tctcgagggg cagacgggct
     9481 acctcattcc cagtaccgat gtacaggtca actttcacaa cgagcggccg atcggcgtgc
     9541 aactgccgcc gagcgtgacg ctcacggtgg cggaaaccga gccccacctc aagagcgcca
     9601 cggcgaccgg ctcgttcaag ccggccagga ccgaaaccgg ggtgacggtg atggtgcccc
     9661 agttcgtcat ggagggcgag agaatcaagg tcagcaccga caccggcgag tacatcgagc
     9721 gcgcatagcg gtcctcaggg aaacttggac cctggcctgg aatttcctcg ccacagccgg
     9781 atcaccgagg tgcggcggga gcccggggca tcgcgaacct cgggttgcag cgtgatgtgc
     9841 cctattccgt agcgatcgtg caacaacgcg cccgccgtgg aaaggatcgc gggccaccgc
     9901 tgcagatcgt cgagctcgat gtgcgcggaa agcgcgatct tgtcgggcgc gatgctccag
     9961 acgtgcaagt catgcaccga aacgaccccc ggcagttccg ccagcgtctg cccgatggcc
    10021 gtcaactcca tcgccggagg cactccctcc atcaggacgt gcagcgtgtc gcgcaatagc
    10081 ttcagggtgc cggcgaggat caatcccgcg atcacgagag agaggatggg atcgatgggc
    10141 agccagccag tcgcgtagat caccgcccct gccagcaggg cagcgaacga actcgcgaga
    10201 tcacccatga cgtgcaacaa agccgcgcgc acgttcaagg tgaccctgcc ccggctcatc
    10261 agccaggcca cggcggcatt caccagcatg ccgatgaagg cgatcagcac cattcccgcc
    10321 gcggcgaccg gcttcggctc gtgcaggcgg actacggctt ccactaccag cgccacgacg
    10381 atcgccagca tgagcacgct gttgagcagc gcaccgatca cctccgcccg cgcccatccg
    10441 taagtgtgct ggtgcccgac cggccgcagc gcaagtcgcg ccgcgaccgc tgcaagcccc
    10501 agggcgaggg cgtcggagaa catgtgtccg gcatcgccca gcagcgcaag cgagccggtc
    10561 cacagcccgc ccgccagctc gatcagcgca taggcgagag tgatcgccag agcgggcaaa
    10621 aagaccggca tgcccggctc gcgtggtagg tggcgatgag tgctcatgag ccgagcggca
    10681 attcaccgtc gtgcaaccca atccccgcta tcgcatgcag cgcgcgcgtg taacgggcgt
    10741 cctcccgcag tctgatcatg ccaccagccg atgcccttcc gtgcatgcgc gcgagccgcg
    10801 cgagactcac cgccggcatc gagtaattca ggccgcccag aagctcgtcc accgccgcgg
    10861 gattgttgca taacaacacc atgtcgcagc cggccgctag cgccgcttca gcgcgttcga
    10921 ccaccgcgcc catggcgctc gccccttcca tgctgagatc gtcgctgaac accacgccgc
    10981 caaacccgag ccttccgcgc agaatctgct tcagccatac cgtcgaaaag cccgccggcc
    11041 ggctatccac ctcgggatag atcacgtgtg cgggcatgat gccgcccagc ccgttgtcga
    11101 ttgcgcgccg gaacggcgcc agatcggcgg cctcgatttc ctcataagcg cgctcgtcga
    11161 ctggtacttc gtgatgggaa tcgccgcgga cgtgaccgtg tcccgggaaa tgcttgccga
    11221 ctccgctcat gccggcctgc ttgaatcctc gcaacaaggc gcttgccaat tcggcaacgg
    11281 catgcgggtc ggagtgcagc gcacggtcgc cgatgacgct gctcgccccg tggtcgatgt
    11341 cgagcaccgg ggccagcgtc aggtccaccc cgtgggcctg cagctcggcc gccagaatga
    11401 agcccgcttc gctggcgagc tgccgggcct gttgcggatc ggtgtcccat gccctgccga
    11461 gctcccgcat ggggggtatc gccgtgaatc cctcgcgaaa gcgctgcact cttccgcctt
    11521 cgtgatcgac ggcgacaatg agcggcgggt tgcgcaaggc gtgaatgccc gcggtcaacc
    11581 gcataagctg ctcggagctt gagtaattcc gggggaacag gatgacgccc cccgccaggg
    11641 ggtgcaggag gcgccggcga tcgtccgccg tcagctccgt cccggagaca tccagcatga
    11701 gcgggccgag cgcgagcgtc ttcgccatca tgcttccagc accacgcagg cgcaggcaat
    11761 attctgctcg tcgctgatcg tcagatggtg gctgacgatg ccgcggctcg taacgagccg
    11821 ttcgaggttg gggtgaaacg cgaaacccgg cttgcccgcg gtgttcggta tcacgctgat
    11881 gcactgcagg gtcacgggat agcggaatcc cgtgcccatg gccttggaga aggcttcctt
    11941 cgccgcgaaa cggttggcca ggaacaacac ggggcgcctc gttctgaggt aggcgggcca
    12001 ttcgagcggc gtcaacacgc gccgcgcgaa gcgctccccg tacttttcca tcagccgcgc
    12061 gacgcgccgg ggttccacga cgtcgatgcc gatgccgtag atcatcgcgc agagtcgatg
    12121 agcgccttca tttcgcgcac ggcccggtcg atgcccacga acaccgattg ggcgacgatg
    12181 gcatggccga tattgagctc gcgtatctgc gcgatcgcgg cgatcggctg gacattgcgg
    12241 tagttcaacc cgtgcccggc gttgacgcgc aagcccctgc tcgcggcgta ttcggccgcg
    12301 gcgcggattc tttcaagctc ctttccccgt ctcctggctg cgctggcatc cgcataagcc
    12361 ccggtatgca attcgaccac cggtgcgccg gcgtcgagcg cggcgtcgat ttggtccttg
    12421 tcggcgtcta tgaacagcga cacgcggata ccggccgctc caagatcgcg gcacgcccgc
    12481 tccacgcgcc tggatttgcg cgccacgtcg agcccgcctt cggtggtgag ctcggcgcgc
    12541 cgctccggca ctaggcagac atcctgcggc cgcacgcgcc gcgcaaacgc gatcatctgc
    12601 ggggtcaccg ccgcttccag gttcatgcgc gtcttcaaca ccttgcgcag gaccatcacg
    12661 tcccggtcct ggatgtgccg gcggtcctcg cgcaggtgca gcgtgatgta gtcggcgccg
    12721 gcggtctccg ccgccagggc cgcctccacc gggctgggat agtcggtgcc gcgcgcctgg
    12781 cgcaaagtcg cgacgtggtc gatattgacg cccaggagga tcataaatcc tgcaggtcgc
    12841 gcagcagttg ccgggtgtgc aggacctggt cggcgaggta atgattgatc agcgtgcgca
    12901 tcagcgccct gctctgctgt tgcgtcactg cgctcgcgaa aatgccggac tgcatgtcga
    12961 tcagcgtctg gcccgacaat tctaccccgt ttcgcggtgc gtcaccgccg cgcaccgggc
    13021 cgcgctcgat gacgtacacg taattgcgct cgcgcgcgac cggctcgtcc cgctccacgt
    13081 cgcggtccag tatcaccgcg taacccagct cgcgcagcag ctcgagttcg aaacgccgca
    13141 gcagagcgaa ctggtccgcg ccgtcgccga gcgcggcaag cgtgccctgg taaatcgtga
    13201 acagccgctc atgcggatcg tcgcgcggca agagcttcag aagcagctcg ttgaggtaaa
    13261 aaccgcagat cagcgcccgt cccttgagcg gggcatagcc gccttgccac tccgcatggg
    13321 tcagcgtgcg cagctcacgc ctgccggccc acgacaggag cagcggctgg aacgggagca
    13381 atacgccgcg caacgaggac ttcgggcggc gcgcgccgcg cgccaccaac gcgacccgtc
    13441 cgtggtcccg gctgtacgct tccacgatca ggctggtttc ccgaaaggga tagctgtgca
    13501 ggacaaacgc cgtccgggct tcctggctgg acttgtcggt cgggcccgcc attctaaaac
    13561 cccaggcgct tgagcatcgc atcgctcttc gcccagccgc gcctgacctt gacccggact
    13621 tccaggaaca ccctgcctcc gaacagccgt tccatgtccc gccgcgcctg cgaagcgatg
    13681 gacttgagct tttctcctcc ggtcccgatc acgatcggtc tctggccttc cttgtccacc
    13741 agtatcgacg cgtggatgtg acgcaagccg ccgtcctccc tgaagcgctc gatttcgacg
    13801 gtggtcgagt acggcaactc atcgcccagt cggcggaaca gcttctccct caacagctcc
    13861 gccgccagga accgctcatc gaccgtggtg agatcatccg catcatggag cggcggccct
    13921 tccggcagca acgatacgac cacttccagc agctcgtcca cttgcgatcc gttcccggcc
    13981 gatatcggaa cgatcgcttc gaattcacgc cgcttggaaa gccggtcgat gaaaggcagg
    14041 agccggccct tctcctttac gctgtcggtc ttgttgaccg ccagtaccac cggcactcga
    14101 gccggcagca accccgacac tttctcgtcg cgctcgtcgt atttcagcgc ctccaccatc
    14161 cacacgatca cgtccacttc ctgcaggctg gcggcgacgc tgcggttcat cgaccggttc
    14221 agcgcgccgc cgtgcccggc ctgatagccc ggcgtgtcga tgaacacgac ttgtgcatcg
    14281 gaacgagtga cgatgccggt gacccgctgc cgcgtggtct ggggcttgct tgaggtgatg
    14341 ctgagcttgc agccgacgag acggttcagc agcgtggatt tgccgacgtt gggacgcccc
    14401 acgatcgcca cggctccggc gcggtgtgcg gacgccgctt caggcgcgga tagcaagctc
    14461 ataagcctga cgcgcggcct cctgctcggc gccgcgccgg ctggcgcctt cccccagcga
    14521 ccgtatcccg agttcgggga tggcgcattc gacctggaac tgctgttcgt gcgattcgcc
    14581 gcgggtggcc accaccgcgt actgtggcag cgcaagcttg cgcgactgca gatattcctg
    14641 cagcagcgtc ttggggtcct tgccggaggt cgcgggatcg atggtatcga gcacattgcc
    14701 gagcaatgtg cgcacgacgc gccgtgcgga gtcgaaaccg ctgtcgagaa aaaccgcccc
    14761 gatcacggcc tccaccgagt cggccaggat ggacggccgg cgcgctcctc cgcttttcag
    14821 ctcgccctcg cccaggcgca actgctcgcc gaaggcgaac tgctgcgcgg cggcggcgag
    14881 cgattgctgg ttgacgatgt tggcgcgcaa acgcgacagc tcgccttcgg tcagccgggg
    14941 gaatttagcg taaagctcca gcgcgacggc gcaattcacc acgctgtctc cgagaaattc
    15001 gaggcgctcg ttatgggacg tgccgtagct gcggtgcgtc aaggcgcggc gcagcagttc
    15061 gagatcggta aagcgatagc cgattctgag acagaagcca tcaagatcca tcaggccgcc
    15121 ggatgccggg ccattggcct atcgtatccg tgaggacgga ctgaagtcca tgcacgcctt
    15181 gaggttgccc acgataggta cacagaccga gtactcggcg ctgagcacga agccgtcacc
    15241 ctccttggtg atcaccaggt ccttgtggga aatcgcattg atgtcctcga tcgtcgatcg
    15301 tttcacgaag gactcctcga tatcgcgccg ctgcccgctg cggagcgtgg ggtcgtccgc
    15361 gatcgcctgc atctgcttct ggatcttgag atactcgaaa taaggcggac cgatcttgaa
    15421 gcccagcagc gcggcgaata tgaatacgac cgcccacatc aggaacccgg acaaggacac
    15481 gcctttttgt ctgtgcatga tcgcccctta tcagttgatg aactgtccga tccgcttgaa
    15541 atcgtcgaaa ttccaccaga tcatgaacgc cttgccgacg atgttggttt ccggaacgaa
    15601 cccccagtaa cggctgtcgc tgctgctgtc ccggttgtcc cccatcataa agtagtgttc
    15661 ctccggtacg gtgcagctga agcccgtctc attgtaggcg caattgtcgc ggaatggaaa
    15721 gagccgcacg ccggtaatat gcacgctcgg cacctcggat tgcaccagta tggcgtggcg
    15781 gtgctcttcc agcgtttcga tcagccgctg cgccgagacg aaattgagcc cggactcgat
    15841 gtaattatac tcgccgtccg gcgcgacctt gacctccttg ccgttgatcc acagccgctt
    15901 gttgcggtag gcgatccggt cgccgggcag cccgaccacg cgcttgatgt agtccagcga
    15961 ggggttctcc ggataacgaa acaccatcac ctctccacgg cgcggttgtt tgacgtcggc
    16021 gatcttgaga ttgatgatcg gcagccggat gccgtaagtg aacttgttca caagtatgaa
    16081 gtcgcccacc agcagcgtcg gcagcatgga gccggacggg atcttgaacg gctcgaccag
    16141 gaacgaccgc agcaggaata ccaccaggat caccgggaaa aagcttttcg gatattcaac
    16201 ccaccagggc tcgggacggc ccggcccgcg ccgacggcgc agccagtagt gatccagcag
    16261 ccatatggcg ccggttgcga ccagcagcag gaacaggacc agcgcgaagt tcattgctgg
    16321 ggactccgct gtgaggcgtg aggggtgagg cgtgaggtga aaacctcaag atcacgccgg
    16381 ctcgtcgcct ctctcctcac tcttcactcc tcacttctcg acctgcagga tggcgagaaa
    16441 ggcctcctgc ggaatttcaa cgctgcccac ttgcttcatg cgtttcttgc cggctttctg
    16501 cttttccagg agcttgcgct tgcgcgtgac gtcgccgccg tagcactttg ccagcacgtt
    16561 cttcctcaac gccttcaccg tctcgcgcgc gatgatgtgc gccccgatcg cggcctgaac
    16621 cgccacgtcg aacatctggc gcgggatgag ttcgcgcatg cggaccgcga gatcgcggcc
    16681 gcggtgctgg gcgttgtccc ggtggatgat ctgggacagc gcatccaccc gctcgccgtt
    16741 gatgaggatg tcgagtcgca ccaggtcgcc ggcgcgatac tccaggaatt cgtaatcgag
    16801 cgaggcgtag ccgcgcgaga ccgattttag cctgtcgaag aaatccatca cgacttcgtt
    16861 gagcggcagc tcgtaggtca gcatgacctg tcgcccgagg tattgcaggt tgcgctggat
    16921 accgcgcttc tggttggcga gcgtgatgac cgggccgaca taatcttggg gcaccaggat
    16981 cgaggccttg atgacgggct cccggatttc ggcgatccgg gccgggtcgg gcagcctcga
    17041 ggggttttcg atctccatga ccgtgccatc gcgcgtgagc acctggtaga ccacggtggg
    17101 cgcggtggtg atcaggttca tgtcgtactc acgctcgagc cgctcctgga cgatatcgag
    17161 atggagcagc ccgaggaagc cgcagcggaa gccgaatccg agcgcctgcg acgattccgg
    17221 ctcgaagcgc agcgaggcgt cgttcaagtg cagcttttcc agcgcgtcgc gcagcgcgcc
    17281 gtaatcaccc tcgtcaaccg ggtagaggcc tgcgaacacc tgcggcttga tttccttgaa
    17341 cccgggcaac ggctgcgcgg cggggcgctg cgccagcgtc agcgtgtcgc cgacgcgcgc
    17401 ggctttcaac tccctgatgc cggccgtgac gaaaccgact tcccccgccg cgagaacgtc
    17461 cttgtatttc gccctgggcg tgaacacccc cagctgctcg cacagatagc cggcgccggt
    17521 cgacatcaac aggatccggt ctttgggctt caggcatccg tccacgaccc gcaccagcat
    17581 cacgatgccg acgtaattgt cgaaccagga atcgatgatg agcgccttca gcggggcgtc
    17641 cgggtcgccc ctgggcggcg gaatgcggct gatgacggct tccaggatgc cggcgacgcc
    17701 ttccccggtc ttggcgctca cccgcagcgc gtcccgggcg ggcaaaccga taatatcctc
    17761 gatctcgccg atcacgcgct cgggttcggc ctggggcagg tccatcttgt tgagcaccgg
    17821 aatcacctcc acgcccagct cgatcgcggc gtagcaattg gccaccgtct gcgcctccac
    17881 gccctgcgat gcatccacca ccagcagcgc gccctcgcag gccgagagcg agcgcgacac
    17941 ctcataggaa aaatcgacgt gccccggcgt gtcgatcagg ttgagcaggt agcgcctgcc
    18001 gtcgtgcgcg cggtattcga gcgccgcggt ctgcgccttg atggtgatgc cgcgctcgcg
    18061 ctcgagctcc atggaatcga gcacctgctc ttccatctcg cgttcggcca gcccgccgca
    18121 cagctggatg aagcggtcgg ccagcgtcga cttgccgtgg tcgatgtggg cgatgatgga
    18181 gaaattgcgg atgtgctgca tgtaaaaggg cacctgacgg tgcccttccg ttcatctaaa
    18241 tctgcgcatt ttatcggaat tcggccagat aagcagtaac ggcggcgcgc tcgagaacgt
    18301 agtgacaaag ttcccgcttg ccatgcgcga gcagcggcac ctttgcgccg tagagccgtt
    18361 cgagcgccgg atcgccgtcc acgtccacca cttcgatctc gaagcgaaag cggccctgca
    18421 aattccgcag ggccgcgatc atttcctcgc agagatggca ggcgtttcgg ctgtaaacgg
    18481 tgagcgtgag cgttcgctgc tcagttgcca tcgacgcgga atggtacgta gagcgaattg
    18541 gcgccgcggc ggatcagcag cgcaacgatc cggcccttgt cgaattgtcc catcacctgg
    18601 ttgaactgtt cgacggactt cacgtcctgg ttgttgatcg cgaggatcac gtcgccgcgg
    18661 cggatgccgg cccgcgccgc cgcaccctgc gcgttctcca ccagcacgcc tccggagacc
    18721 ttgagttcgg ctcgctgggt ctcggtcagg tcggacaggg tcaacccgta ctgactgggc
    18781 gaagctgcgg gcggcttgcc gccgcgccgc ccctggcgcg cggcgcggtc atcctgcatc
    18841 tccgccacca ccacctgcag gtcacgcgcc tgcttgttgc gccagatctg caacgtcacc
    18901 ctcgatccgg gcttggtcgc accgacgacg cgcggcaggt cctccgaagc gttcacgacc
    18961 ttgccgtcga aacggaggat gacatcgccg gcctcgatgc ctgccttgtc cgccggcccg
    19021 cccttctcca ccgagttgac cagcgcgccc tggagccggg acagaccaaa gccgtcggcg
    19081 agctccttgg tgacgggctg gatcacgacg ccgatccggc cgcgcgtcac cttgcccgag
    19141 gtgcgcaact gctgtgcgat gtcgttcgcg acgtcgatcg ggattgcgaa ggagagtccc
    19201 atgaaaccgc cggtgcggct gtagatctgc gaattgatgc cgaccacctc gccccgcagg
    19261 ttgaacagcg gcccgcccga gttgccgggg ttgaccgcca cgtcggtctg gatgaacggc
    19321 acgtaatttt cctgcggcag cgagcgccct ttggcgctga cgatgcccgc ggtcacggtg
    19381 ttctcgaaac cgaacggaga gccgatcgcc agtacccatt cgccgaccct gagccggttc
    19441 gggtcgccaa accggacggc cggcaggttc gatgcatcga tcttgatgag ggcgacatcg
    19501 gtgcggcggt cggcgccgat gaccttggcc ttgaattcgc gcttgtcgtt gagcttgacg
    19561 gtgacttcat cggccgcttc gaccacgtgg gcgttggtca ggatgtaacc atcctgactg
    19621 atgatgaagc cggagccgag cgattgcgac tggaattcgc gcggcccggg ttggggtatg
    19681 aagcgccgga aaaactcgta gaagggatcg tcctcctgca ggttcggtat ctgcggcaac
    19741 aggggattgc gcaccgactg cgtggtgctg atgttcacga ccgcggggcc ctgtttctcc
    19801 acgagttcgg tgaaatcggg gagttgcgcc gcgacggcgg ccggcacgat gaaaaacagc
    19861 gcaacaagga tgcgcaactg caggcgcttg aacataaacg atctctacgg ttatagcttg
    19921 ttgtgtcggg aatgacttcg ggtgcggcaa gccgcgaatc ctaccggggc gaagctccct
    19981 tgaattcgag cgaattggcg atctgcatga cggtggcggc gggggcttct cccagcaccg
    20041 tcaccatgta atccgaaacc ggacggatat agatgttgac ggctccctgg tgcgacaggc
    20101 tctgggctgg acggaccttg ggcagcggct cgatgaacac cgacacggcg gcgaggccgt
    20161 cggaatagac gatatgcgac accatggcgg cgcgcccggc gatcgatcgc ttctgctcgg
    20221 tgagcttcct gaagccggcc ggctgctggg tcagcaccca tccggtctcg gccgggatct
    20281 cggtggtcgg gaatcccgcg tggtcgactt tccagttgcg gctctttgcg gcgtagcggg
    20341 atctcaccct gtcgcggttg aagctgccgc cgatcttcag ctcggtaaag acgaacgatt
    20401 ccagttgctc attcttctcg ttgaacgtgc gcgcgcgcaa cggcagtccg gaagcgtcgt
    20461 cggcgcagaa ttggtggcca tagcgcagct tgtccctcgg ctcgagcgtg atcacctggc
    20521 aatcgtggcc cgccacccgg tccttcctgt ccacgttcac gacatagttg tcggacacgc
    20581 cggtgagctg ctcgggcagc atcgccggga agcgacgctt gctacgcttc tcgatgatga
    20641 ccgtcttgct ggccgggaga tagcagacga cctggtcatt ggtgcggatc acctcgcgca
    20701 ccggtccttc aagcgtttcc agcttctcga attcccctcc ggcagcgttg acgtaatgga
    20761 cgatgcggga agtctcggtc tggttggggt ggcggtaaac gaaagtgccg ctgtaattga
    20821 gctggcgcga ggcgctcgcc atcttcttga gccaggcgag cccgtcctgt ggcgcgccgg
    20881 tctcggcggc caggaccggc gcggcgagcc ccaggacgac gatgaaccac gccttcttca
    20941 cgctagcgcc gttccggctt gacgaaaaca attgcccccg cctcttgcgc cttgcgatgt
    21001 gccttgacca tacacggcgg atggcgccaa ggttggaacg gcactagcgt cccgtggccg
    21061 gttgcgtact cgatacgctg cggatatagg gggcaagtcc ctggatcgcg gtgctgggcg
    21121 aaaactcctg gtgcgcgatc aggtattcgt tcattttccc gtcgctgggg acgctggcta
    21181 attgtgtcga gggggttggg acgggagggg gggcggtttg ggctttggcg agttccggct
    21241 ggggggcgag gggattgttg gagaaggcga tccagccaac cagcgcgacc gcggacaggg
    21301 aggcggccgc agacagggca taggcggtga tgcgcctggc cagatggctg cggcggggcg
    21361 ccagcaccgt gggctccttg gcgaggcgct caatcaggtc ctggctgaaa ttcagcgaca
    21421 gggcgcgctc accgcgcatc acgtcgccgg ccacatggaa agcatgccag agataaagca
    21481 actcttcgtc ctgcttcatc cgcgcgatct gctcgtgcgc ctggcgcttg tccagctcgc
    21541 cgtccatcaa tgccgatatc ttgtccatct caccatctcc tgtccctccg ggtgccgagc
    21601 agcggccgca gttttgccgc gatcgcctcg cgtgcccgga aaatgcgcga ccggacggtg
    21661 ccgatcgggc agttcattac attcgctatg tcctcgtagc tcaggccctc aatctcgcgc
    21721 agcgtgatcg cggtcctcag ctcctgtggc aactcgtcca gcgtttggtt cacggtttcc
    21781 gcgatctgcc gggtcgcgag ctcgttctcc ggcgtgttga ggtcgcgcag ctgatcgccg
    21841 ttttcgaagc cctccgcctc ctcactgtcg atgtcagtcg tggtcggcgc ccgccgcccc
    21901 atcgccacca gatagttctt ggcggtgttg atgccgatcc ggtagagcca agtataaaac
    21961 gcgctgtcgc cgcggaacgt cggcaacgcg cggtaggcct tgatgaaggc ctcctgcgtc
    22021 acgtcctcga cctcggccgc gtcccggatg aatcgcgaca gcagccgggc gagcttgcgc
    22081 tcgtacttgc tgaccagcag ctcgaacgcc ttcttgtcgc cgcgctgcgc gcgctcgacc
    22141 agctgctgat cgacttcgcg atcgctcatc aaacccgtcc ctgatcttcg aattgttacc
    22201 caggtcgagc ggtggcgctg gctggacaaa acccacagta tacccgcccg gccggtccac
    22261 gcggaacgcg aagcctgatc gttaatgcac tgacagggct gcataaattt agttcactcc
    22321 cggaaagggt tatttcacaa gaacgccccc tcccccggtc cccgaggtgg aaacaggacc
    22381 tcggctgtca gccggagcgg ttgccggact ggcgcagacc cggcgggatc agcagtcggt
    22441 cgagggcgtc aaagccccat tggaccccca gcgccatcac agcggccggc accgcccccg
    22501 cgagcagcag aatgtgatcg ttgaccgcga gcccggcaac gatgcgctcg ccgtagccgc
    22561 cggccccgat gaaagcggcg atggtggcgg tgccgacgtt gatgacggag gaagtcttga
    22621 tgccggcgag gatggttcgc gccgccagcg gtagctcgat cagctgcagc cttgcgccct
    22681 cgccgattcc cagcgccgtt gccgaatcct tgagcgagcg cggcacgccg gcaagcccgg
    22741 tttccgtgtt gcggacgatg ggcagcagcg agtacaggaa cagcgcaatg atggccggca
    22801 cggtgccgat gcgatcgagc gccgcgatca aaaaggcgag cagcgcgagc gacggtaccg
    22861 tctgcagtat gcccgcccct cccagtatcc aggctcgcgc cggtggcttg cggttggccc
    22921 atatgcccag gggcactccc gtcgcgacgc ccagcgcgag cgaagcgaag accaggagca
    22981 gatgctccgc tgtcaggcgc cagaagtccg cgccgaacag cgcggagagg aatgacctgc
    23041 gctcgcgctg cggtgccgca gcgcccgtcg agtaacggcc ggccaggaaa tcctcggcaa
    23101 tcctggaaaa cggcagcgcc gacaactcgg ctgccgcgtt catgcgcacc atctgttccg
    23161 aatcgatccg gcctgtcagc tcctgcaatg cccgccagga gcgcggatac ttcgcaggca
    23221 gatcggcgcg gtacagaatc accgcatcat atgccgggaa gaagccccga tcgtcctgca
    23281 gcacccgcaa ccggtagcgc gcgatcttgg cgtccgtcga atagacgtcc acgacgtcga
    23341 cctggccggc cgcgagcgcc tcgtacgcca gcccgtgatc gaggccgcgc gcttccacga
    23401 acggtaactg atagcgctgt tgcagcgcgc gccagccatc cccgcggttg aggaattcct
    23461 gggaaagcgc gaaccgcagt tgcgggtgtc cggacagatc ggatattcgc gtgaccccga
    23521 gcgccccggc gcggctctcc agcatcgcca gtgcgtaact gttgctgaat ccaagcggca
    23581 cgcctgcggc gagcccgtgg cgggcgagct cgctgttgag cccagccggc ccgggcacgc
    23641 ttttcagccc cagcagctcg aaggcgatcg tgccggtgta ttcggggtag gcgtggatcg
    23701 cgccgctttt cagggccgcg aacaggatgg cggtatttcc cagcccctga tggtggacgg
    23761 ctcgcgcctc ctccggccgg gccaacgtgc gcgtgacgat ctcggccagg atgtaggact
    23821 cggtgaagcg cttggatccc acgttgatga cgggctgggc cagggccaac gcgggaaaca
    23881 gcaacaatgg aaggagacgc atcatggcaa gcgtcggcga ttcccgccca gtatggctga
    23941 acttcggcgg cgctggcaaa atacccctga ctcttccgcg aaagccgcgc catgccgcca
    24001 cgcgattatc cccttgagcc gttctggatg ccgttcaccg ccaaccgcca gttcaaggcg
    24061 gccccgcgca tcctggtttc ggcgaaggac atgcattacc tcgccgagga cggccgcagg
    24121 atactcgacg gcaccgcggg cctgtggtgc gtgaatgcgg ggcactgccg cccgaagatc
    24181 gtcgaggcga tccggcgcca agccgagacc ctggactatt cccccctgtt tcagatggga
    24241 caccccgggg aattccgcgc cgccgccctc gtcgcccagc ttgccccggc cggcctcgat
    24301 cacgtgttct tcaccaactc gggctccgaa gcggtcgata ccgcgctgaa gatcgcgctg
    24361 gcctatcacc gcgcccgcgg cgagggccat cgcaatgtgc tcgtcggtcg cgaacggggt
    24421 tatcacgggg tcggcttcgg gggcatctcc gtcggaggca ttccggccaa ccggaaggct
    24481 ttcggcaaca tgctcgcgcg ggtcgatcac ctgccccata cccactacct cgagcacaac
    24541 gccttcagcc gggggcagcc ggcgtggggc gcccatctcg ccgatgatct ggaacgcatc
    24601 gttgcgctgc acgacgcctc caacatcgcg gcggtgatcg tggagccggt cgcgggatcc
    24661 accggcgtgc tggttccgcc caagggttac ctggaaaggc tgcgcgcgat ctgcgacaag
    24721 cacgggctgc tgctcgtttt cgacgaggtg atcaccggct ttggccggac cggccacgct
    24781 tttgccgcgc aaaccttcgg cgtgacgccc gacatgatca cgttcgccaa aggcgtcacg
    24841 agcggcaccg tgccgctcgc cggcgtactg gtgcgccgcg ggatctatga agcgatactc
    24901 gagagcgcgc cgcccgatac agtcgagctc ttccacggct acacctactc ggggcacccg
    24961 ctcgccaccg ccgccgccga ggcgacgctc gcgctctacc acgaggaaaa gctcttcgac
    25021 cgcgcccggg aacttgcgcc ctatttcgag caggcggtgc acagcctgag gggcgcaagc
    25081 cacgtgatcg acatccgcaa cttcggcctg atggcgggaa tcgagctcga accgctgccg
    25141 gggaaacccg gggctcgcgg ccaccaggtg ttcctcaagt gcttcgagca gggcgtcatg
    25201 acccgcgtca cgggcgacat cgtcgcgctt tcaccgccat tcatcatcag caggagccag
    25261 atcgacgagc tggcgggaac gctgggggcc gccgtgcgcg aactggagaa gaaatagccc
    25321 cctagatgcg ctgcatcgtg cgctcgccga gcgcgacgag acagtggtcc agccactccg
    25381 cggtgagttt ttcctgcatc gaattctgct tgaggcgctg ctcgagcgcc ttccagtcca
    25441 cccggtcgag cggctggtcc tggttgaagc aggaaaacac gtagcttacc ttgccggtcg
    25501 cctggtccac atgcggttgc aggcactgcg cgcagatctc cttcatcatg cactgcatcg
    25561 gcgaattgat cgagccgatc ccgacatggc ccgccttgag gtggggcttc agcacatcgt
    25621 ggcgcgcgcg cgccaccgcc gccatcatgc ggtccgagcc gatggcgatc aggcggtcgg
    25681 catccttgaa cgcgatgggc tgctcgccca gcgcgccgct cgcgtaggcg tgcatgcctt
    25741 gcacgatgtt gccgacgaaa ctcctgtccg gtggtcgctc cggagtgaaa ccgggctctt
    25801 cgtcgcagca ccaaaccacg aggtccgcgg ccgcctcgat ctcatgcacc ttgtagcggt
    25861 ccgtcatgtg cttgtagccg gcgaaataga gcacccggct gccggcgcgg cgcagcgcct
    25921 ggccgattga gaacagcacg gcgttgccga ggccgccccc ggcgaggacg acggtctcgc
    25981 ctgggggaat ctcggtggga ttgccggtgg gccccatcag gactaccggc tcgcccggct
    26041 tgagcagcac gcaaagatcg ctcgatccgc ccatctccag cacgatggtc gagacgagcc
    26101 ctcgctcgcg gtccacccac gcgccggtga gcgcgaggcc ttccatcgcc agccgggtac
    26161 cttcggcctc gcgcgcggac gtctcgtagt tctgcagacg gtagaactgt cccggccgga
    26221 agcgccgcgc agcgagcggc gcccgcacca cgacttcgac gatgagcggc gtcagccgca
    26281 ccacttcgtg aacggtcgcg cgcagctccc cgttgaggcg ggcgatgaac gcgtcgtcgc
    26341 tcagcatgga tgccggcccg cggcgttcga gcacgcggct gaccgccgga tagccctgct
    26401 tggcgctgcc cagcgccttg accacattgc cgaagtacga cgggtggacg tcgccgaagt
    26461 agctcatgaa gcgaccgtca ccgcgccgtg acagcaacac gcgcgggcgg gccggtttgg
    26521 agatcgccct ttccacggca accggatccc cgttttcgtc gcacgcctgg aagtagcggc
    26581 catcgagcct gaaattcacg ggatcctcgc gcgcgaggac cgtgttgggc tgggtgccgg
    26641 cagcgatgag gattgttctc gccggcaact cggtttgccc gatctccgcc cacttgccgt
    26701 tttcaccgcg tcgctgcacc gagaccttga gcgcacgggc gtgcccctct tcgtccacct
    26761 cgatggcgag cgggctcagg ccttccgcga aacggatgcc ctcctccagc gccttgtgca
    26821 cttcctcatg gttgagggta taggagggac tgtcgatcag ccgcttgcgg taggcgatcg
    26881 tgaccccgcc ccacgattgc agcagttcga gtatgcgcgg ggcgcggtcc tcggccaccg
    26941 cgcgcatgcg ctccgagcgc agggcacgcg cgtgagccag gaactcgtcg gcgatgcgtc
    27001 tttcctcctc gtcccaggcg accctcgccg cggtctcgcc gtgctcgcgc gccagcttct
    27061 cgtatcgttg caggaacttc tccacctgta gcgggtagta agcgagagac tcggttgcgg
    27121 tgtcgatcgc ggtcagaccg cctccgacca ctaccaccgg cagccgcagt tgcatgttgg
    27181 cgatcgagcc cggcttggcc gcgccactca gctgcagggc catgagaaaa tcggacgccg
    27241 cgcgcacgcc gcgcgcgagc ccgttcggca gttcgagcac tgtcgggcgt cccgccccga
    27301 tggcgagggc cacatggtcg aagcccagct caaacgcttc tttcgcggtg agcgtgccgc
    27361 ccaagcgcac gcccccgtac atcgcgaact cgcgccggcg ctcgagcagc agccgcagta
    27421 tcttgaggaa gtttttgtcc cagcgcacgg tgatcccata ttcggcgacg ccaccgaatc
    27481 ccgccatgac gcgccggtcg agcgcttcgt agagctcgcg cacgtcgcga accggctgga
    27541 acggcactcg cgccccgcgc gcatccacgc ccgagagcgt gggcggcagc ggctcgatct
    27601 tgaggccgtc gatgccgacc gccacatggc cgtcattcat caggtagtgg gcaagcgcga
    27661 tgcccgccgg ccccatccct gcgatcagca cgcgcttgcc gctcggggcc ttgggataag
    27721 gcgcgcggag gttgagcggg ttccagcgcg tgagcaacga atagatctcg aatccccacg
    27781 gcagttcgag cacatccttc aacgtccgcg tctcggcctg cgggatattg accggctcct
    27841 gcttctggta gatgcacgac ttcatgcagt cgttgcagat gcggtgaccg gtcgccgcga
    27901 ccatcgggtt gtccacgacg atgatggcga gcgcgccgat ggcatgcccc tcggccttgg
    27961 cctgttgaaa ctcggaaatt ttctcctcca gcggacagcc cgcgagcgtg tcgccgaacg
    28021 gacttttctt gaacgaagcc gctcccgcgc cggtggcgcc tttttccctg aggcccttgg
    28081 agcaggaatc cttgccctgc tcgtggcacc agatgcagta attcacttcg tccagggcgc
    28141 cggtgaggtc ggtgccggcg tcggtcagct tgaatccgtc acgccgccgc agatggtccg
    28201 cggcgaggcg gtggctcttg tagccgccgg cttcctcggt gacgacgggg acgagccgtt
    28261 gggggtcgag cttggcgggc gccttgaaca gcacgcctcc ccgacgccgc ttcctgcccg
    28321 cctcggaata cacggcccac gcggcatagc gtaacgccgt ctcgagccgc gccgcgtgcg
    28381 gcgcctcgtc gtcctgccac tgggtgacgt gacgcgcgaa ggcaagctcg gtaaagggct
    28441 cgccaaacgc ccgctcgagc tcccgttcga gcgcgaggcc gtcgtagtcc gaggcctctc
    28501 gcggcttgat cctgtgcatc gctttgcgct gcacgaattg ccgcttcacg gtgtagagcg
    28561 gcgcgagttc gtgatgtctt gcggcaagca cctggacctc cgcctcgatt ccgaagagcc
    28621 gcgcgatgaa ctgctccacg tgcggcgcca gcgccagcag cagctcggac tcctgtttcg
    28681 gcgccagcgc tgcgggctcg gcacgggcgc gcagtaaccg ctcgtgcagc cctgcgtcgc
    28741 cctgctccag ccaagtgagg aagttgcggt cgagcctcaa caatccttcg cgacggtaga
    28801 ggtcgtcgaa ggcgagtccc gagccggtta caggaatggt gtcagcagcc acggagggcg
    28861 cccggtcagg gtcaaaaata aaacattata tcaatcggtt agccgctcgc tcataaccgt
    28921 gcgttctatt catatatgac gcgccggatg ccgtccggtt gcggatatgc gtgtgcaata
    28981 atgcggacgg ccgaaaccgg gagacttgga gcacgacggc gatgctcgat gttcgcatga
    29041 gccagggaag gcgcatcctg ctgtggctga tgctggccgt gcttgccgcc ctcgtgacct
    29101 atgtggcgtt ccgcggctat ttcagccccg agttgctgct gaatttctcg aatacctttt
    29161 cctgctgaag ggacgcgggg cgctatgcgg cgcgcgccgt gagactgcgc tccgctgctc
    29221 tgaccgtgtt gacgatgacc atggcgaccg tcatcggccc taccccaccc ggcacgggag
    29281 taatccagcc ggcgaccctg cgtaccgctt cgaaatccac gtcgccggcg aacttgccgt
    29341 ccggcaggcg gttgatgccg acgtcgatca ccgcggcgcc tggcttcacc atgtcgtcgc
    29401 ggatgagccg cgggcggccg gccgcggcca ccaggatgtc cgcgcgtcgc gtgtgttccg
    29461 cgagatcccg cgtgcggctg tggcagatgg tcacggtcgc gccgcgtgcc atcagcatga
    29521 gcgccatcgg cttgccgacg atggggctgc gtcccaccac caccgcatcg cggccgtcca
    29581 cggcgacgcc ggcgcgctcg aggagaacga tcacgccgcc tggcgtgcag ggctcgaacg
    29641 aaggccggcc ggccaccagc gcgcctagac tcgcccaggt cagcccgtcg acatcctttt
    29701 caggcgccat ggcctgcatg acccgtccgg catcgaggtg ggctggtaac gggagctgca
    29761 gcaggatgcc gtggatgcgc gggttgcggt tgaggctttc cagcgtggcg agcaatgcct
    29821 cctgcgggca atcggcgggt aaattgtgca cttcggaatg aacgccggct tcgtcacagg
    29881 cccgtacttt gttgcggagg tagattttcg aggcgggatt gtcgcccacg aggatcgcag
    29941 ccagcccggg gcgcacgccg gatccggcca gcgcggcgac gcgcgccttg agccgggagt
    30001 agatttcacg tgcggcggcg gccccatcga gcaccaccgc gggcgccgcg catcgtgatg
    30061 ccgtcatgtc agggtctggc gggcccggaa gcgcgcgccc ccggcgcggc ttccagcacc
    30121 tggatgaaaa tctcgtcctg ccggatcatg ccgagctcgc tgcgcgcccg ctcttcgatc
    30181 gcctcgagtc ccactttaag atcgcgcacc tcggcttcca gcgcgttgtt gcgggcctgt
    30241 aactggcggt tggtctcgcg ctgggcgcgg atctgctgct ccacctccca cacccggagc
    30301 cagctgccct tgcccagcca cagcggatac tggatcagca cgatcaacgc tgcaagcgcc
    30361 agggccagca ctctcatcgg gtgaggagtg aggggtgagg agtagtaagg cgcaaaaggc
    30421 ggggggaaat atcaccggca acccctgcca ggccaggaac gacggggttt gctcggcgct
    30481 gctgacaccc ctctcgcctc acccctcaca cctcaccgta gctggtaaaa ggccgcacgg
    30541 cccggatagc tcgccgtttc ccccaagtcc tcctcgatgc gcagcagctg gttgtacttg
    30601 gcaagacggt ccgagcgcga cagcgagccg gtcttgatct gcagggcgtt cgccgccacg
    30661 gcgatgtcgg cgatggtggt gtcctcggtc tcgcccgagc ggtgcgatac caccgcggtg
    30721 tagccggcgc gcttggccat ttcgaccgcg gcaaacgtct cggtcagcgt gccgatctgg
    30781 ttaaccttga tcaggatcga gttggccacg cccttttcta tgccctgctt caggaagcgg
    30841 gtgttggtca cgaagacatc gtcgcccacc agctgcactt tccggcccag gcgccgggtg
    30901 agcagcttcc acccgtccca gtcgtcttcc gccatgccgt cctcgatgct gatgatcgga
    30961 tacttctcga cccaggcggc gagatagtcc acgaactgct cggcgctcat ggagagccct
    31021 tccgaagcga gtgtgtattc gccgtcctcg aagaacccgg agctggcgca atccagcgcg
    31081 agcgccacct gctcggcggg ccggtaaccc gccgcttcga ccgcttccag gacgatcttg
    31141 atcgccgctt cgtgcttggg cagccgcggc gcgaagccgc cctcgtcgcc caccgcggtc
    31201 gaaagcccgc gcccggcgag cagcttcttc agcgcgtgaa acacctcggc gccgcagcgc
    31261 agcgcctcgc ggaacgaggc aaaccccacc gggacgatca tgaactcctg catatcgagg
    31321 ctgttgtcgg cgtgcgcgcc gccgttgatc acgttcatca tcggtaccgg catggacatc
    31381 gcccccaccc cgcccaggta acggtgcagc ggcaagcccg attcctcggc cgctgccttg
    31441 gctaccgcaa gcgacaccgc gagcacggcg ttggcgccga ggcgcgattt gttctcggtg
    31501 ccgtcaagat cgatcagcgt cttgtcgacg aagctctgct ccgtggcatc taccccgatg
    31561 accgcctcgc agatttcggt gttgatgttt tccaccgcct tcagcacgcc cttcccgcca
    31621 tagcgggcgg catccccgtc gcgcaactcg atcgcttcgc ggctgccggt ggacgccccc
    31681 gacggcacgg cggagcgccc cagcacgccc gattcgagca gcacgtcggc ctcgacggtg
    31741 ggatagccac gcgagtccag gatctccctc gcaatcacgt caacgatcgc actcattgtt
    31801 gttcctgtct tggttcgatg tttttcagca ccgcggctgt cccgacagaa ggttttccgc
    31861 gaacccgcga ctcttgaccg ttgcgtcgag cgccttgagc gtctcgagca gcggcttcat
    31921 cagtttcagc ggccaggcgt tgggcccgtc cgagagcgcc ttctccggat tgggatgggt
    31981 ctccatgaac aggcccgcga tgccgctcgc caccgccgcg cgcgccagca ccggcacgaa
    32041 ttcgcgctga ccgccgctcg cgcttccctg cccgccgggc agctggaccg aatgcgtcgc
    32101 atcgaatacc accgggcagc cggtattgcg catgatcatg agcgagcgca tgtccgagac
    32161 caggttgttg tatccgaacg acaccccgcg ctcgcacacc aggatgttgt cgcgccggct
    32221 cgccgcctta gctttctcta ccacgttttt catgtcgccg ggcgcgagga actggccctt
    32281 cttgatgttg accggcttgc cggcggcggc tacggcgcag atgaaatcgg tttgccggca
    32341 cagaaaagcc ggtgtctgca ggacatccgc cgtcgccgca gcggccgcga tctcgtcgat
    32401 ttcgtggacg tcggtcagga ccggcacacc gatcttgttc ctgacttcat ccagaatctg
    32461 aagacctttt tccatgccca ggccgcgaaa ggattttatg gatgtcctgt tggccttgtc
    32521 ataggacgac ttgtagatca aaggcagccc cacctggctg cagatttcct tgagttcgcc
    32581 ggcggtgtcg agcgcgaggt cccgggattc gaccacgcac gggcccgcta tcaagaacaa
    32641 cggctgatcg aggccggctt tgaacccgca cagctcgatc acgtcgcggg agggctgtcc
    32701 accgcgctgc ggcgacggtg ccgattgcga tcgtcgcggg cgagaagtca cggtgccggt
    32761 cctcaggcga tgcttttcag ccgcccggtg tcccctgcca gcccggcctc ccccggccgg
    32821 gcgtaagtca acgcggcctg cacgaacgcc ttgaacagcg ggtggccatg acgcggggtc
    32881 gaagtgaatt ccggatggaa ctggcagccg acgaaccacg gatgaccggg caactcgatc
    32941 atctcgcaca gctcgtcttt ggccgacacc ccgcttacct tgagacccgc tgacttcagc
    33001 cgcggcaggt agtgattgtt gacctcgtag cgatggcgat gacgttccat gatgcgttcc
    33061 tgcccgtaga tgcggcgtac cgccgaatcg ggctcgagca cgcactcctg cccgccgagg
    33121 cgcatggtgc cgccgagatc cgactgggcg ctgcgtcgct cgatgcgtcc gtcccggtcc
    33181 tgccactcgg tgatcaaggc aatcacgggg tgcggcgtat cgggatcgaa ttcggtgctg
    33241 tgggcgccga ccagccccgc cgcgttgcgg gcgtactcga tgatcgcgag ctgcatgccg
    33301 agacagatcc cgaggtacgg gaccccgttc tcacgcgcgt agcggatcgc cttgatcttg
    33361 ccttccaccc cgcgcttgcc gaagccgccc ggcaccagta tcgcgtccat ggcgccgagg
    33421 cacccggtgc cgttcttctc gatgccttcc gaatccacat aatggatctt gaccctggcg
    33481 ccggtgtgga tgccggcatg gatcagggcc tcggtgagcg atttgtacga ctcggtgagg
    33541 tccacatact tgcccacgag ggcgatattg accgttcgct gcgggttctc cagcgcgtgg
    33601 accaaccgct cccagctcga gaggttggcg ggcggcggat tcagccccag cttgcggcac
    33661 acgatgtcgt cgagcttctg ggcgtgcaac atcgccggga tcttgtagat gctgtccacg
    33721 tccacggcgg agatcaccgc ctccactgcg acgttggtga acaaggcgat cttccggcgc
    33781 tcgtcgtcgg gcagcttccg gtcggcgcgg cacagcaaca cgtcgggctg aatgccgatc
    33841 tcgcgcaact ccttgaccga gtgctgcgtc ggcttggtct tgatctcgcc ggccgccgcg
    33901 acgtagggca ccagcgtcgg gtggatgaag cagtaattat cgcgcccgag ttcgatcgcc
    33961 atctggcgga tcgcctcgag gaagggcagc gattcgatgt cgcccaccgt gccgccgatc
    34021 tcgacgatcg ccacttccgt ctccgccgcc ccgcggcgta tgaagtgctt gatctcgtcg
    34081 gtgatgtggg gaatcacctg gacggtgccg ccgaggtagt cgccgcggcg ttccttcttg
    34141 atcacgctct cgtagatctg gccggtggtg aagttgttgc gcttcgacat cttgacgtgc
    34201 aggaagcgct catagtggcc gagatcgagg tcggtctcgg cgccgtcctc ggtgacgaaa
    34261 acctcgccgt gctggaacgg gctcatggtc cccggatcca cgttgaggta gggatccagc
    34321 ttgaccatcg agattttgag gccgcgggac tcgaggatgg cggccagtga ggcggcggcg
    34381 atgcctttgc ccagggagga aacgactcca cctgtgacaa agatgtattt agtcgtcatc
    34441 gcataagccg gcagtttcgc tattttatcc aaaaatggcg cggcgtccaa tgatttggat
    34501 gcgatagcgc cgtgcggaca ttggggctag gtcttgcggg aactgccggc ctctcgtgcg
    34561 aggtagagcc aggtctcgac tacgctgtcg ggattgagag acaggctttc gatgccctcc
    34621 tccaccagcc acttcgcgag gtccggatgg tcggaagggc cctgcccgca gatgccgacg
    34681 tacttgccgg cgcgccggca cgcctggatc gcaaggtgca acatctgctt caccgccggg
    34741 tcacgctcgt cgaactgctc cgcgatgatg ctggaatccc ggtcgagccc cagggtcagc
    34801 tgggtcatgt cgttggaacc gatggagaac ccgtcgaaat gctcgaggaa ctggtctgcg
    34861 aggatcgcat tcgagggaat ttcgcacatc atcacgacgc gcaacccgtt ctcgccgcgc
    34921 cgcagtccgt tcccggccag cagttccagc actttttttg cttcgttcac ggtgcgcacg
    34981 aaaggcacca tgatctcgat attggtcagt cccatctcgt cacgcacctt cttgagcgcc
    35041 cggcactcga gctcgaagca gtcgcggaac gaggggtcca cgtagcggcc cgcgccgcgg
    35101 aaaccgagca tcgggttctc ctccctcggc tcgtagcgcg cgccgccggt gaggctcgag
    35161 tactcgttcg acttgaagtc ggacagacgc acgatgaccg gtttgggcca gaatgccgcg
    35221 cccagcgtcg ccacgccttc cgccagcttg tctacgtaaa acgtaaccgg atcgggatag
    35281 ccggcgctgt gctcctcgac ggccaccttg acgtccgccg ggaggtcggg ataggcgagc
    35341 acggccttgg ggtgcactcc gatcatgcgg gcgatgatga attcgagccg tgccagcccc
    35401 accccttcat tgggcaggcg ctggaactcg aacgccagct ccgggttgcc gacgttcatc
    35461 gtgatcttca ccggtgcttt cggcatgctg gagagcgaga ggtcgatcac ttcggtctcg
    35521 aggacgccgc ggtagacgaa gccggtgtcg ccctcggcgc aggaaacggt gacgggacgc
    35581 ccgtccttca gaacctgcgt cgcgtcgccg caccctacca ccgccgggat gccgagctcg
    35641 cgtgcgatga tcgcggcgtg gcaggtgcgc cccccgcggt tggtgacgat cgccgcagcg
    35701 cgtttcatca ccggctccca atccggatcg gtcatgtccg tgaccagcac gtcaccctga
    35761 cgcaccgccg acatctcgtc ggtcttcctg acgacgcgca ccgggccggc tccgatcttc
    35821 tggccgatcg ccctgccggt cgtcaacact tcggaacgct gcttgaggcg gtaacggcgc
    35881 agcgtatcga gcttctcgcc cgctttcacc gtctcggggc gggcctgcag gacgtacagc
    35941 ctgccgtcga tgccgtcctt gccccactcg atgtccatcg ggcgccggta atggtcctcg
    36001 atgatcatcg cgtagcgcgc cagttcctcg acctccgcgt cgctgatcga gaagcggcgc
    36061 cgctcgcgct cgggcacatc caccgtcccc accgaccggc cgggctgtgg cgcgtccaca
    36121 tacaccatct tgactgcctt tgaaccgagc cccctgcgca ggattgcatg cttcccgtcg
    36181 cgcagcgcgc gcttgtaaac gtagaactcg tcagggttga ccttaccctg cactaccgtc
    36241 tcgccgagcc cgtaggccgc agtgatgaaa acgacctggt cgaagcccga ttcggtatcg
    36301 agggtgaaga tcacaccgct ggccccgaga tcactgcgca ccatgcgctg aatccccgcg
    36361 gacaatgcga tctcgtcatg caggtagcct ttgtgcactc ggtaggagat ggcgcggtca
    36421 ttgtagaggg aggcgaacac ccgtttcacg gcgacgtaca ggttgtccag accatgcacg
    36481 ttgaggaagg tttcctgctg gccagcgaag gaggcgtccg gaaggtcctc ggcggtggca
    36541 gaagagcgca ctgcgaacga ggcgtccgcc ccggcgccgt cggcgagtct ctcgtaggcc
    36601 tgcgcgatct cctcatggag tcgctgcgga aaatcctgcg cgacgatcca gccgcggatc
    36661 tcctccccgg ccctcgcgag ggccgccacg tcgccaacgt cgagcttgcc gagacgatcg
    36721 gcaatgcgcg cgtccagctt tttctgggca aggaactcgc ggtaggcacg ggcagtcgtg
    36781 gcgaaaccgc ccggtacccg cacgccggcg gcgccaagct gactgatcat ctcgccgagc
    36841 gaggcgttct tgccgccgac gcttgccaca tcattcatgc gcagcgtcgc gaggtcgatt
    36901 accagggggg ggttgctcat attgagccgc tgaattgtgc atcgcaggaa aaaaagttat
    36961 tttaccggca aaagccgctg ctgccagccc cgatgacgcc caaacgcacc gcgttcttcg
    37021 tatccgaccg caccggcatc accgccgaga tgctgggaca cagcctgctc acccagttcg
    37081 acggcgtgcg tttctacgag gtgaccctgc cgttcgtgga tacggtggaa aaagcccgcg
    37141 aagcggtgga gaggatcaat cagcagggcg caacggacgg cgtgcgcccg ctcgtgatca
    37201 gcacgcttgc gcgcaccgag atcgcgcaag tcatcggcca gtcgaacgcg ttgtttctcg
    37261 actgcttcga gatcttcatt tcccccctcg agcgcgagct cggcatcaag gcgtcgcacg
    37321 ccgtcggccg cacccacagc atttcggatt tcgtcaacta ccaccaccgc atcgaatcgg
    37381 tgaattactc tctcgcgcac gacgacggcg tgtcctaccg cgacctcaac gacgccgacg
    37441 tcatcctggt cggggtctcg cgttgcggca agacgccgac ctgtctgtat ctcgccatgc
    37501 aattcggcat tcgcgcggcc aattatccgc tgataccgga ggatttccgc ggcacgcagc
    37561 tgccgtccca gctgcgaccg ctgcgcggca agctctacgg gttgaccatc cggcccgaac
    37621 gcctgcagga gatccgcaac gagcgccggc ccggcagccg ctacgcgacg ctcgccaact
    37681 gcgagttcga ggtgcgggaa gccgaggcgc tgatgcggca ggaaggcatt ccgtttctcg
    37741 acgccaccag caaatcggtg gaggaactgg caacgaccat cttgcaggag gcgaagctgg
    37801 tgcggcgcat ttattagcag tgatgaatgc agagtgcaga atgcagaatt atcccactct
    37861 gcacccatca ctctgcactg cccttagctg ccgtacccgc tcgaacaagc acacagctgc
    37921 cgccgctgcg acgttgagcg actcggccgc gcccggcatt gggatcgaga ccacggtgtc
    37981 cgctgccgac agcaggcccg cgctgaggcc ggcgccctcg tttccgagca gaagggcgat
    38041 gctgcccgtc aggtccgcgt cgaatacgga ccgcggcgag cgctgttgcg tcgcgacccg
    38101 cttgcccggg taggcgcgtg cgacgccctg caggtcgact ccctcgtaaa tcgcaagcgc
    38161 gaaatgcgcg cccatcgccg cccgcagcac cctaggcgac caggcgtgca ccgatccgcg
    38221 cgacaacaat acgtgcctga cgcccgctgc ggcgctcgtg cgcaggatgg agccaaggtt
    38281 gccgggatcc tgcaggtctt ccagcatcac gcaggtgtcc atttccagcg ggaccggttg
    38341 cggacgcggc gtttcgacca cagccagtat tccggtcggc gtcaccaccg gagcaagctg
    38401 ccggaacagc gcgtcgccca tcacgacgac atccacccct ttcattgcgt ccagcagcgc
    38461 ccgtattccc ggcctctcca tcccggaacg gctgaccgcg atctgctcag gccgcccgac
    38521 gtgctcttgg taggcggcga caagatgcac gccatcgaga actgaacgac ccgcccgcct
    38581 gcgctcgctt gaggattcca cgagccggcg cagggccttg aagcgcggat tggcggccga
    38641 gtcgatccgt ttcacatggc tcctgcggtg gcgacgacga tattgagcaa cccgagtgcc
    38701 aggttgaccg caaccgcgat gcggatctga ttgagcgcca cggcgcccgc tttccagtcc
    38761 cgcgcggtga ccgcgtgcct gagcgtgggg tatggcgaga agaatacata agcgaaaatc
    38821 agcgtcatcg tcgcgccgac gtagagcatg agatggacgt tgagcggaac ccgcccgagt
    38881 tccgagactg cggccaccat gtggaaaccg ctgcccagaa tcagtgcaat ggacacccat
    38941 acccactgga aaaagcgctc cagtgtcgcc gcccacagca gcaggcgcag cggcggctcg
    39001 agccctgaag cggccggtcg caggcaaagg taggcaaaga acatgccgcc gatccacacc
    39061 accgcgccga gcaaatgcag catgaccgca attttcatgc ccctgttcct ttgtcgagca
    39121 gcgcgcgcac cggggcgaaa ctgcggcggt gcgcgaccga gacgccgtgc tcgcgcagcg
    39181 cctccaggtg caagcgggtg ggatacccct tgtggcgatc gaaaccgtat tgggggtgcc
    39241 gctggtgcag ggcccgcatt gcatcgtcgc gcgccacctt ggctagtatc gaggccgcgg
    39301 cgatcgccgc caccgtcgca tcgcctcgca cgacagcttg caccggcacg tccagtcgcg
    39361 gccgatgcag gccgtccacc agtacccgcc gcggcgtcac cgcgagtgct tccaccgcgc
    39421 gccgcatggc gagaagactg gcctgtagaa tattgagggc gtcgatttcc tccacgcttg
    39481 cacttgccac cgcccatgcc gctgagcgca tgcggatttc cggggccagt gcctcacgct
    39541 ggcgcgcagt aagcttcttg gaatcggcga gcccttctat gacgcattgg cggtctagta
    39601 tgacggccgc tgcaaacacc ggtcccgcga gcgggccccg ccccgcttca tccactccgc
    39661 aaacaagagt catggcgcaa tgccgttccg tatcaggggg atcagcgcgg acgcgatgcg
    39721 cccggcgctg tcctggcgca gcgcctgggc catggcggca aagcgcgcct cgagccggac
    39781 gcgcaccgcg ctgtcgaaca tcagattgag caccgcctgc gagaggttct cgggagtcgc
    39841 ctcgtcctgg aggaattctg gaacgacgaa ttccccggcg acgatgttgg gcagcccgac
    39901 ataaggctgg tagcggcggt tattcatgat ccaccacgac aggcgcggca tgcgataggt
    39961 gatcaccacc ggccgtccca gcagcgccgc ttccagcgtc gcggtgcccg aagccgccag
    40021 caccacatcc gccgcggaca tcgcctcgtg cgcgtggccg aagagaacgg tgacgttcaa
    40081 tccgtcacgc tggcgccggt agagctcggc ctcgaaccgg tcccgcgtgg gccggttcac
    40141 cagcggcacg agaaactgcg cgtcaggcag gcctgccgca atctggatcg cggtctcgat
    40201 gaaaagatcg gccatctgtt cgagctcgct catccggctg ccgggcagca acgcgatgat
    40261 ctttgccgcg gccggcaggc gcagttgctc gcgcgcggcg gcgcggttcg aaactcggct
    40321 cagcatttcc gccaggggat gccccacgta ggtaacggga atgccggcct gttcgtagag
    40381 cgcttgctcg aacggaaaca ccaccagcat gcggtcgacc gcgcgtttga tcgtgcccat
    40441 gcgacgggcc cgccatgccc agatctgggg gctcaccagg tgtgcggtcg gaatgcccgc
    40501 ggcccggagc ctgagctcga ggccgaggtt aaagtccggt gcgtccaccc cgatgaacag
    40561 cgccggcggt tgcccgagga accgcgccgc cagccgtcgg cggatcccga ggatctcgaa
    40621 atagtggcgc agcacctcta catagccgcg caccgcgagc ttttccatcg gatatagcac
    40681 ttccatgcca gccgactgca tgcgcgggcc gccgattccg gtaaagcggg cgtgcggcag
    40741 ccgcgcccgc aacgccctga tgaggtgcac gccgagctgg tcaccggacg cctcgcccgc
    40801 aaccacgccg atggtgaccg gagcctcggc catgtgacgc cgcgttaccg gacgatgccg
    40861 cgcgtggaat ccgcgaggaa gtccagcacg acctggatct cagggcactg cttcagctgg
    40921 cgcgccagtt cccgccgggc ctggtcaagc gtcaggcccg atcgatagag cgtccggtat
    40981 gcccgtttca gcctagtgat ggtctcggcg ctgaatgccc ggcgcttcaa cccctcggcg
    41041 ttgatgccaa atggtctggc cgggttgccg gaaaccgtga tgaacggggg tacgtcctgc
    41101 agcaccaccg tgccggcgcc ggtgatgctg tgcgcgccga tatggcagta ctgatgcact
    41161 gcggtcaggc cgccgagaat cgcaaattcg ccgacgtgca cgtggccgcc cagctgtgtg
    41221 ccgttggcca tcacgacgtg gctcccgacc tggcagtcgt gcgcgaggtg cacgtacgcc
    41281 atgatccagt tgtcgtcgcc gaggcgcgtc acgccggcgt cctgcgcggt cccgcagttc
    41341 agggtgcaga actcgcggat ggtgttgtcg ttcccgatct cgagccgggt cgcctcgccg
    41401 gcgtatttct tgtcctgcgg cgcctcgccc agcgacgcga actgaaagac gcggttgttg
    41461 gcgccgaggc acgtgtgacc ggtgatgaca gcgtgcggcc cgatccagca accgggtccg
    41521 atttccacat gagggccgat cacggcgtac gggccgattt caatatcgga cgcgatgcgc
    41581 gccttgggat cgacgacggc ggtggaatgg atcttcacgg gagctgcgga aggaggacag
    41641 gcacgaaggc agtttattct ccccccttga tcgaacgcac cgtgcacatg atctcggact
    41701 cggccaccat tatttcgccc acccaggcgt gggcagagaa tttccagagg ccacggacat
    41761 ggcgcgtgat cgtgacttcc agccgcaacg tgtcgccggg aacgaccggt ttcttgaagc
    41821 gggcatggtc gataccgacg aaaaaataca ccgagcgctc gtccggcttg gcattttcgc
    41881 tgtggaacgt cagtatggcg gccgcctgcg ccatggactc gatgatgagc acgccgggca
    41941 tcaccggata gttgggaaaa tgcccctgga agaacggctc gttgattgtc acgttcttga
    42001 ccgcgacgat cttctttccc ggctcgcagg aaaggacgcg gtcaaccagc aggaacgggt
    42061 aacggtgggg caggtacttg aatatctgat tgatgtccat gatgctcacg acctgctcct
    42121 tttccgcttc ttcggtttcc cttccagggc gcggatgcgc tgtgtcagtt cgtagaggtg
    42181 gcggagctgg gcggcgttcc tgcgccattg cgggtgcggc tcgaacggat aggcgccggt
    42241 ataggtccca gggcggtcga tcgacttggc tatgagcgtg tacgcggaaa tctccacgtg
    42301 gtcggcgatg acgagatgcc cggcgatgcc cgatgctccg ccgatcctgc aatagcgccc
    42361 gatctttgcg ctgccggcga caccggcgca gccggcgatc gcggtgtggg cgccgatgcg
    42421 gacgttgtgg ccgatctgta tcaggttgtc aagcttgacg ccctcctcga tcacggtgtc
    42481 atcgagcgct ccgcggtcca ccgtggtatt ggccccgatc tccacgtcat ttccgatcac
    42541 tacgcgcccg atctgcggca ccttgaccca gcggtcctct tccaacgcga tgccgaatcc
    42601 gtcggcgccg atgacgaccc cggagtggag tatcacacgc tccccgatca cgcagtcgtg
    42661 atagatcgta acgttggcgt gaagcgtcga accagagccg atcgtcacgt cgtcgccgat
    42721 gcagcagcca gccccggcaa cgcagccggc gccgattcgt gcattgcgtc cgactaccac
    42781 gcaaggcccg atctcggtgt cctccgcgat cttcgcggag gagtcgacca ctgcgctggc
    42841 gtgcacgccg ggccgggggg ggcgcgccgg attgaacaaa gccgacacgc gcgcaaagta
    42901 ggcgtacgga ttggcacata cgatgcgcgg taaccgcgtg gcatcgcgtg ccgactggcc
    42961 cacgatcacg gcgcctgcgc gagtcgtcct gagctggggg aggtaacgtt cattggtgag
    43021 gaaggcgatg ctttcgggcg tggcgctttc caacgtggcg acccggcgca cctcgacccc
    43081 ggaatcgcct gcgatctccc ccccgaaacg cgccgtgatc tcgcgcaggc tgtaggattt
    43141 gcgttcgctg agtgtcatga ccgccaggcc ggggatgccg cgaattgcat gaaggacgcc
    43201 gcgtgctcga cgcgctgtcg ggcgccgttt atttgacgtc ggccagcgcc ttgagcacct
    43261 tgtccgtaat atcgatctgg ggactgcgat acaccgcttc ttggacgatc aaatcgaatt
    43321 tttcagcctc cgcgatctgc cggattacgc ggttggcacg ttccaataac gacgatattt
    43381 cctgattgcg gcgaatgttg aggtcctcac ggtattcacg cagcatgcgc tggtactcga
    43441 cgccctgtcg cgcgatctcc tgctccttgg ccctgcgttc actctcgctc atcgtcatcg
    43501 cttccttttc gagctggccc tgcagcgcct tgatctgggc ctcgcggcgt tgcagttcct
    43561 gctggcgcgg agcgaattct ttttcgatct gcctggccgc gcgttccgcc ggggccgact
    43621 cgcgattgat gcgctcggcg tcaacgtaac cgatcttgat ttcggccgcc ccgaccgtcg
    43681 cggcggcgaa gcccagcatc agggcggctg atgtcgcata tagggacttc aatgtactct
    43741 ccttgcggtt ggtttgcccg tcctgcgggc aacgcgtcgc gggctcagaa taccccgccg
    43801 atggtaaatt gaaacgcctg tgtgctgtcc ccgggctgct tgcgcaacgg cttggcgacg
    43861 ctgaccttga gcgggcccag gggcgatacc cataatagcg acagtcccgc cgaataacga
    43921 agctcgttgc tgtcgatctt gtcaccgacc actgctgtgt cgaaaaatgt gctcagccgc
    43981 accgagcggt cgttctccag gcccgggaac ggaaaaaaaa gttcggcgtt tcccaccacc
    44041 ttgctgctgc cgccacgggg gttaccattg ctgtccttgg ggcctatagt ataagacctg
    44101 aagccacgta ccgaactgac gccgcccgca aagaagttct tgaagaacgg cagcggtttg
    44161 gcgccgtaac cgtccccgtg gcccccctct ccgttgagca ggaaggtgag atgacgagtc
    44221 agcgggaaat aacgctgata ctggtaggat aatttgtagt actgcaagtc accgcccggc
    44281 agtccagatt cgatgccggc cttgtgcagc gtccctttgg tagggtaaat caggctgtcg
    44341 cgcccgtccc gcgtccagcc ggcggtcgca aacaggttgc tattgctggg gccgaaagtc
    44401 gtcacgtaat ccttgtaaat cagcgggctg tcgggaaaaa tcgtgatgtc gacattctcg
    44461 tatcccaacc cgtaatggat ggtgtcgatc tcggacaccg ggacgcccag gcgcagttgc
    44521 cctcccagcg tcttggtttc gtaacgggcg agaccgaagt ccgatggggt aaccagccgg
    44581 tagtacacgt cgaagccctg gctgatgccg tccactgtga agtacggatc ggtgtaggac
    44641 aaggagtaga ccgtgttgat cttgccgctg ttgatcgatg ccgatacgtg tttgccgctt
    44701 ccaaaaatat tgtcctgcga aatcgagccg gaaagaataa gcccttcgcc gctgccatag
    44761 cctgcgccaa acagcagcaa tccggtgggc ttctcgacca ccgagatgtt gacgtccacc
    44821 tggtcggtcg tgcccggcac cgccggtgtc tcgagattca cctcggtgaa gtaaccgagt
    44881 ttgtcgatac gctgcttgga cagggtgatt ctctccgcgg aataccaccc tccttccagc
    44941 tgccgcattt cgcggcggat gacctcatcg cgcgagcggg tgttgccgct gatgttgatg
    45001 cgacgcacgt agacgcggcg gccgggatcg atgaaaaaag tgaaggcggc ctgctgcttt
    45061 tcccggttga gctccggaac ggcgttgacg ttggcgaagg cgtagccgtc gttgccgagg
    45121 cggtcggtaa tgagcttggt cgattcggta aggcgcgccc gggagaacac ctcccccggt
    45181 ttgatctgga tcaacttgcg cacctcgtcc tccgggatca gcatctcgcc cgccaccttg
    45241 atgtcggaca ccgtgtactt gctcccctcc gtgatgctga tcgtgaggta gatgtcctgc
    45301 ttgtccgggg taatcgagac ctgcgtcgaa tcgatgcgga actcgagata accccggtcg
    45361 aggtagaacg agcgcagcga ctcaagatcg gcggaaagcc tctggcgcga atattggtcg
    45421 tgcttgctga accaggtgag ccagcccggt gtgcgcagca cgaactggcc caacagctcg
    45481 ctctcgcgga aggtctgggc gccgatgatg ctgatctggc ggatcttcgc aacccgcccc
    45541 tcctcgacgt tgaagttgat cgagacgcgg ttgcgctcca ggggggtcac ggtggtgctg
    45601 acgttgaccg cataatagcc gcgacccaga tactggcgtt tcagctcctg ctccgcgcgg
    45661 tcgagcaggc ccttgtcgaa gatgcggctt tcggcaaggc cgacctggcg aaatccggtg
    45721 atgacctgct ccttgttgaa ctcgtgcatg ccggtgaagt cgacctgggc gatcgacggc
    45781 cgttcctgca ccaccaccac cagcacgtcg ccctcccgtt ccaggctgac gtctcggaag
    45841 aatccggtcg cgaacagcgc ccgtagcgcc tgggccgctt tttcctctgt cagggtatcc
    45901 ccgaccttga ccgggaggta gctgaagacg gtgcctgcct cggtgcgctg gatgccttct
    45961 acgcggatgt ccttgatgac gaagggctcg attgcggttg ccgctgccgc atgcagcgca
    46021 agcagcagcc aggcggcgcg gaaccagcgc atgaagtcag ccgcttacca ggaggcgggt
    46081 tatgtcgtta tagagggcga aggccatcag cgtgaacagc aacgccatgc caacgtgctg
    46141 gccgatctcg atggcccgct ccgaaatcgg atggcctttg aaaatctcga cgatataata
    46201 catcaaatgc ccgccgtcca ataacgggat cggcagcaga ttcaacacgc cgaggctgat
    46261 gctgatcagc gccagaaaca gcagatacga ggccaccccg ctttgcgctg attgtcccgc
    46321 gtagtcggcg atggtgatcg gaccggagag gttcttgagc gagacgtcgc cgacgatcat
    46381 cttgccgagc atcatcaggc tgaacaccga ggtgtcccag gtcttgtgca gggccttggc
    46441 gagggcttcg atggtgccat agcgcacttc gacgatcatc ccggccagcg ccgagcggtc
    46501 cacccgcggc gcggcgccaa tcctgccgat cttccggccg ttctcgatca cgacttcggg
    46561 agtgaccgcg atggccggtt gcagcacgcc gtcccgcctg acctcgacga cgagcggtgc
    46621 gcccggttca ctgcgcacga tgttcaccac ctggtcccag gtcatgatgc gccggttgcc
    46681 gatcgcaacg atctcgtcgc ctgccttcaa tcccgagcga tgagccgcgc caccgctcac
    46741 cacgtctccg atgaccggtt tcagcttcgg ctgatgccgc atcaggccaa gcgtgcgcag
    46801 aaaatcgctg tcgaggtccg ccggcgtcag ccccgacagg tcgagcttgc gccagacaat
    46861 gctgccaccg gcgccgcgca cctcgatcgt gacgacggac ttctgaacgg ctccctgcag
    46921 cagtatccag cgcgcgtcct gccaggtcgc gaccggctca tcgcctatcc tcaccagcgt
    46981 atccccggcg gcaaagccga ccagacgggc cgcgctgcca gcgggcggct cgtcgatcac
    47041 cggcctgatg cccggcaccc cgtgaacgaa caatccccag tagagcgcga tcgcgagcag
    47101 gaaattggct gccggccccg cgacgacgat ggccagacgc cgccagactg actggcggtt
    47161 gaacgcccgc ggcaagtctt cgtgcgccac ctgtccctcg cgttcatcca gcatcttgac
    47221 atagccgccg agggggaagg cggcaatgac ccactcggtt ccgtcgcgcc ccagcgatct
    47281 ggcccacagc ggcttcccga acccgaccga gaaacgcagc accttcacgc cgcaggcgcg
    47341 cgccacgagg tagtggccga actcgtggac cacgatcagg cagcccagcg ctgccgcaaa
    47401 ggcgatgagg gtggtgagca gtgtcatgat ccggcgctcg tgctcgatgg atcgcctgga
    47461 agctcaggac cttaccgtct ggcgcaagtg cgccgcggcg ccggcggaac gcgacgcatg
    47521 cagccagacc tcggcctgct cgcgggcgag gcggtccgcg gcaaacacgt cttcaaggcc
    47581 ggtcaccggc ccgcacggga ctttcgagag caccgtctct atcaatgcgg gaatccggcg
    47641 gaatccaagg gcgccggaaa ggaaactcgc aaccgctatc tcattggcgg cgttcaacac
    47701 ggtcggcgcg ctgccccccg ccttgagcgc ttcgtaggcc agtcgcaggc agggaaaacg
    47761 ctgaatatcc gggcgctcga aatgcagtgc gccgacgcgc gcgaggtcga gatactcgac
    47821 gccggccgcg atgcgctcgg gaaagcccag cgcaaacgcg atgggggtgc gcatgtcagg
    47881 atagccgagc tgcgccatca ccgacccgtc gatgtactcg accagcgagt gcaccacgct
    47941 ctcgggatgg atcacgacct cgatgttttc gggatccacg ttgaacaacc agcgcgcctc
    48001 gatcacttcc agccccttgt tcatcatggt cgccgagtcc accgagatct tgcgccccat
    48061 cgcccagttg ggatgagcgc aagcctggtc gggggtcact gcatcgagct ggcgcagcgg
    48121 aagggagcga aagggccccc cggacgcggt cagcaggatg cgccgcacgc cggctgcgtc
    48181 cggggcggca taaccgcgcg gcaatgcctg gaatatcgca ttatgctcgc tgtcgatcgg
    48241 cagcagcgtc gcccggctcg cgtgcaccgc gtccatcaat acggcgcctg ccgtgaccaa
    48301 cgattccttg tttgccagca ggactttctt gcctgaccgc gccgcggcca gcgtcgggcg
    48361 tagcccggca atgcctacga tcgccgccat caccgtatcc acgtccggaa accccgccac
    48421 ttcctcaagc gcttccacgc cgctgcgcac ctcgcaatcc agccccgcct gccgcactcg
    48481 ttgccgcaag tcctgcgccg cagcggcttc cagtacaacg gcggtgcgcg ggcggaattc
    48541 gaggcattgc ttgaacaagg tttccgcctg gttgcgggcg gtcaacgcca ccacgtcgaa
    48601 ccggttgcgg tggcgcgcca ccacgtcgag cgtgttgacc cccacgctgc ccgtggatcc
    48661 cagtatggtc aggcgctgct tgtcgttcat ggaacgggaa aacctagcct gcatatggca
    48721 gcaacagcgc tgcaaccggc atggctgcgg tcaggctgtc tatccggtcg aggatgccgc
    48781 cgtggccggg cagcagcgtg ccgctgtcct tgaccccggc taggcgcttg acccacgact
    48841 cgtataggtc ccccacgacg ctcatcaacc ccacacccgc gaacaacacc accccgccgg
    48901 tgccatgcca ccaaccccat cccgctgcgg cgctggatag gacgacataa tacactgcca
    48961 ctgccgcccc ggcgcccgct aacccttccc atgtttttcc cggactgatg tgaggtgcca
    49021 aagggtgctt gccccacgcc ctgccgctaa gatacgccgc ggtatccgcc acccacacga
    49081 cgcccagcac agcgagcagc tgctcgggat ccgactgcaa tcgggccagg gcgagccagg
    49141 ccggcaccag aacgatccaa cctgcggcgc acagtgcgag cggcgaccgc aagcgccagc
    49201 gttgtgccag ccaaggcaag gcgatgagca accaaaatac gcagccggag ccatagatca
    49261 aggcttccgc aacggtgaga cggccggtat aaggcgcggc gcccgtgacg agcagccaga
    49321 ccgcgaccgc cgaaacggac acgaggccgg cgaaaatcca gcgtggggcg cgcctcaggc
    49381 ctgccagcgc gccccattcc cagctggcag ccaggagcac tggcaggagc agcaccgccc
    49441 accatcggtt gggcaggaac aacagcgccg agatgcatac tgcgagcagt acggccgctg
    49501 tggccagtcg cgcggtgaac atgaatccga caagccgtaa cggccgccgc cgagatctca
    49561 atcggcggag cggttgttct cgacgcggaa cgccgcaagc ccagcatcgg ccggcagctg
    49621 ttcgctggtg cggccgaaac ggcgctcgcg ctgctggtag gaaacgatgg cacggtcgag
    49681 cgccgcggcg tcgaaatccg gccacaaaat atcggtgaaa tagagctcgg tgtacgccag
    49741 ctgccacaac acgaagttgc tgatgcgctg ctcccccccg gtccggatga aaaggtcggg
    49801 ctccggcgca tagcacagcg cgaagtacgg ggccaggtcg cgctcgccga aaccggaagc
    49861 cagttccggc cgctcgcgca gcatacggct cactgcctgc acgacatccc agcggcctcc
    49921 gtaattggcc gcgatggtga gcgtcaagcg ctggtttccg gcggtgagcg cctcggcttc
    49981 ccggaccagt tgccgcagct tcggctcgaa gcggccgagg tcgccgatcg ccttgaagcg
    50041 gacgccgttt tcatggagct ttccgacctc ctgctcgagc gccatggcga acagttgcat
    50101 caggaacgac acttcttccg ccggtcggcg ccagttctcg ctgctgaacg cgaacagggt
    50161 gaggaactcc acgccccgct cgacgcaggc gcgcacgacg gtgcgcaccg cgtccagccc
    50221 gcggcggtgc ccagccacgc gcggcagaaa acgctgctta gcccagcgac cgttgccatc
    50281 catgatgatg gcgatgtgac gcggaatacg gcgcgcgtcg ggtatctcga gtgtggagct
    50341 ggcgaaggtc ttcatcagat ggccatcagg tcggcttcct tctgttgcag cagtttgtcg
    50401 atctccacga tgtggcgatc ggtgagcttc tggatctcat cctgcgcccg ccgctcttcg
    50461 tcctccgcca ccttcttctg cttgagcagc tccttcagat gatggatggc gtcacgtcgc
    50521 acgttgcgca ccgccacgcg ggcgttttcc ccctcgtgtc gcaccacctt ggtgagctcc
    50581 ttgcggcgct cctcggtgag cggcggcatc ggaacccgta ctgtctcgcc gctggtcgca
    50641 gggttgaggc cgaggtcgga atcgcgtatc gccttttcga tcacgttcgt catcttcttg
    50701 tcccacggat tgacaccgat ggtccgcgca tcgagcagtg tcacgttcgc cacctgcggg
    50761 atcggggtcg gggtgccgta gtagtccacc atgatgtgat cgaggagccc tgcgtgggcg
    50821 cggccggtgc ggaccttcgc gaggtcgctc ttgagggtct ccaccgtctt tttcatcttt
    50881 tgttccgcgg tcttcttgac gtcggcaatc atggtccacc tcccgcgtgt gtcttccctt
    50941 aattatgcag gaaagtcccc tcgtcctcgc ccatcaccac gcgtttcagc gcggccggct
    51001 tgaagatgct gaacacgttg atcggcagct tctggtcgcg gcacagtgtc agcgccgtgg
    51061 catccatcac cttgaggttc ttgatgatcg cctcgtcgaa ggtcaggcgc ttgtaacgca
    51121 tggcgtcggc atgggctttc gggtcgtcgg tgtagactcc gtccaccttg gttgccttga
    51181 gcacgagatc cacgttcatc tccatgccgc gtaatgcggc agcggtatca gttgtgaaga
    51241 acggattgcc ggtgccggcg gcgaagatga ccaccttccc ttcctccaga taacgcatcg
    51301 ccttgccccg gatgtagggc tcgacgacct gctcgatgtt gagcgcggac tgcacgcggc
    51361 tcaccagact cacgcgccgc atcgcatcct gcagcgccag cgcgttcatc accgtcgcga
    51421 gcatgcccat gtaatccgcc gtggcccggt ccatgtgcga ggaggccggc gccacgccgc
    51481 ggaagatgtt gccgccgccg atcaccacag ccacctcgac accgaggttc acgacctcag
    51541 cgatctcgcc gacgatgcgg tcgacggtct cccggttgat gccgtagctg tcctcgccca
    51601 tcagcgcttc cccgctcagc ttgagcagga tgcgtttgta ggcgggtgtc tgcggcacgg
    51661 tgttctcggg ttgcgttgcg gggacggact gagcagcgcc ctcacaccgc ctggctgacc
    51721 tgcgacatga cttctgctac gaaatcgtcc tttcttttct ccaggccctc gcccaccacg
    51781 tagagcgcga aaccggacac cgaggcgcct tccgacttga gcagttgctc gatgctctgt
    51841 ttgtcgttct tgacgaacgg ctggccgagc aacgtcacct ctttgaggaa cttctgaacg
    51901 gcgccttcca ccattttctc gacgatgttg gggggcttcc ccgatccggc ggccttggcg
    51961 gccgcaatcg agcgttcctt ctgcaccagt tcagccggca cagtggccgc agacagcgcg
    52021 accggcttgc tggcggcaat atgcatggcg agatcccggg ccagcgtatc gccgcctccg
    52081 gccacgtcca ccagcactcc gatcctggcg ccgccgtgga tgtaggtcgc cagcttcccc
    52141 tgtgcggtca tgcgcacgaa acggcggatc gacacgttct cgccgatctt gccgacgagt
    52201 gctttgcgcg cctcttccac cgtcgccccc cggtacggga gccgggacag cgctcccacg
    52261 tccgcaggtc gctgagtcgc cgccagttct gccagcgcgc cggcaaaacc caggaattcc
    52321 ccgttcctgg cgacgaaatc ggtttcgcag ttgacctcga ccagggcacc gctctttccg
    52381 tccggggcaa tataggctgc aaccgcgcct tccgccgtga cgcgggaggc cgccctgctc
    52441 gcccggttgc cgagcttgac ccgcaggatt tcttcggcct tgcccatgtc gccattggcc
    52501 tcggttaggg ccttcttgca ctccatcatc ggagcatcgg tcttttcgcg cagctccttg
    52561 accatgcccg cggtaatttc cgccattccc cgccctcact ggttaaaaaa aaggggctcg
    52621 cgccccggaa gcagcttccc gagaccgctt caagcctcct cgacctcaac gaattcgtcg
    52681 ccgccgcgga cgatctcctt caagctgtcc tgccggccct cgagcaccgc gtcggcgata
    52741 ccgcgcacgt agaggcggat cgcccggctc gaatcgtcgt tgcccggtat cacgtaagcc
    52801 acgccttccg gcgtatggtt ggtatccaca acgccgatca cggggatgcc gagcttgttg
    52861 gcctcggcga ccgcgccctt ctggtagccg acgtcgacga cgaacaacgc gtctggcaga
    52921 ccgttcatgt ccttgatgcc gccgaggcta cggtcaagct tggcgatctc ccgctggtag
    52981 tgtagcgcct ctttcttcgg cagtttctcg atcgagccat cctgcatcat ggcttccatg
    53041 tccttcagcc gtttgatcga ctgcttgacg gtcttgtagt tggtgagcat gccgcccagc
    53101 cagcggtaat cgacatacgg cgagccgcaa cggctcgcct cttccctgat gatctcgcgc
    53161 gcctggcgtt tggtgccgac gagcaggatc gtgcccttgt tggccgcaag ctggcgcacg
    53221 aatttcagcg cctcctggta catcgccagc gttttctcga ggttgacgat atggatcttg
    53281 ttgcgatgcc cgaaaatgta cggcgccatc ctcgggttcc agtagcgagt ctgatgcccg
    53341 aagtggaccc cggcttcgag catctcacgc atcgtgaccg acatgcttcc tgtctcctgt
    53401 aagggttgag tctccacccg ccgcggggtc gccacgatgg cgaccaccct cacgctatgg
    53461 cggatgtgcg aactgggctt ttccgcagtg gattcaaacc aactgagtaa ggcggaaaaa
    53521 gctaactaat tattttaaca caataaaaga tcgctcttca agattatcgg cgcgagtgcg
    53581 tggggcgccg atctggacta caatccgctg tccttgaaaa accaggcatc aactcttccg
    53641 tcgccgcggc gcggtccgcg ctcaatgccc gtcagcatca agtccccgca ggaaatcgag
    53701 aagatgcgca ttgcggggcg gctcgctgcg gaagtcctcg acttcatcgc ccctcacgtc
    53761 aggtccggca cgaccaccgg cgaactcgat cgtctttgcc acgagtttat ttcccaagag
    53821 ctgaaagcga cccctgcgcc tctcaattac gccccacccg gctatcaacc gtttcccaag
    53881 tcgatctgta cctcggtgaa ccatgtcgtc tgccacggcg tgcccggcga caagaagctg
    53941 cgcaccggcg acatcatcaa tatcgatatc acggtcatca aagacggctt ccacggcgac
    54001 accagccgca tgttctacgt cggccatcct tcaatccaag cgcggcggct gtgcgaggtg
    54061 acgtacgagt gtctgtggcg cgggatcgag tcggtgcgtc cgggagacca tctgggcgat
    54121 atcgggcacg cgatacagaa ccacgcggag agtcatggtt acagcgtggt gcgcgagttc
    54181 tgcggccacg gcattggcag gaaattccat gaggagccgc aggttctgca ctacggcagg
    54241 ccgggcgtcg ggttgaagct ggagccgggc atgaccttca caatcgagcc catgatcaac
    54301 gccggcaagc cggacgttcg ccagctcgcc gatggctgga cggtggtgac caaggaccac
    54361 agtctgtcgg cgcaatggga gcataccgtg ctggtgaccg aatccggcca cgaagtgctc
    54421 acgctctcgg cgggctcacc ggcgaagcct gcgctgaatg cctgacgcat ggctggactc
    54481 caggccgtga ccgctgctgc gcctgcgttg caggcgcgcg tcggccagtg gaaacagaag
    54541 cttgccgcgc tgcgcctgca actgcgcgat cagttcttca tccaccaatc gccaaaagac
    54601 ctgctccggc gccagtcgac gacggtcgac cgccagctca aggaaatgtg ggcaagccac
    54661 gccatgccgc gcaacgtagc gcttgctgcc gtgggcggct acgggcgcgg ccagctcttt
    54721 ccctgctccg atgtcgacct gctgatcctc ctgccggcga cgaccgacgc cgccctcgcg
    54781 cgcaagatcg aagacctgat tggcacgtta tgggacatcg ggctcgaggt cggccacagc
    54841 gtgcgcacct tggacgaatg cgtcgccctg gcggcgacag acgtcacggt tcagacaacc
    54901 ctgctggagg cccgcttgct cgccggcaag cgcgatttgt tcgcgaggtt cgtcaagcgc
    54961 atgcgcgatc tactcgatcc cgcagagttc atgcaggcga agctgctgga acagcagcag
    55021 cgccacgtac gctacgctgc gaccaccctt gaacccaaca tcaaagagag cgccggcggt
    55081 ctgcgtgatc tccagacgat actctggatc gcgcgcgcgg cgggaatcgg caagagctgg
    55141 cgggagctcg ctcaccgcgg cgtgataact tctgcggagg cacgcgaaat caaccagcac
    55201 gagcagttcc tgcaatcgct gcgcatccgg ctgcactatc gcgcgggccg gcgcgaggac
    55261 cggttgctgt tcgacttcca gaccgccctg gcgcacgaga tcggtttgca cgacaggccg
    55321 catcgcctgg cgagcgagca gctgatgcag cgctaccacc gcgtcgccaa ggcggtctcg
    55381 cagctcaacg ccatcgtgct gcagaacctg gatgcgcgca tcgtcccccc ccgggacaac
    55441 gagtatcgcc cgatcaacgg gcgcttcggc atccgggacg agctcctgga agcgcgcgac
    55501 gagcagctgt tcgagcgcca cccctccgcg atcctggaaa gcttcctgct gcttcaacag
    55561 caccatgaac tgaagggcat gagtgcggcg actctgcgcg cgctttggcg tgcgcggcgg
    55621 ctgatcaacc cagcgttccg gcgcaacccg gccaatcgcg aacaattcct gaatgtcctg
    55681 aaaagccctt ctcacgtgga acgggcgctg cggcgcatga acgagaatga cgtgctcggc
    55741 cgctatctgc ccactttcgg gcgcatcgtc ggccagatgc agcatgacct ctatcacgtg
    55801 cacacggttg acgagcacat tctgcgagtg atccgcaatt tgcggcgctt ctcgataccc
    55861 gagctcgcgc acgagttccc tctgtgcagc cggctgatga gcggctttgc ccgccccgaa
    55921 gtgctgtacg tcgcggcgtt gtttcacgac atcgccaagg ggcgcggtgg cgaccattcg
    55981 ctgctcggga ccgtggacgc gcgccgcttc tgccgggaac acggattggc gcgggaggat
    56041 gtcgagctcg ccgcctggct ggtcgagaac catctggtga tgtccataac cgcccagaag
    56101 caggacatcg cggacccggc ggtggtgcag gcattcgcga ggcgcgtcgg cgacgagcgc
    56161 catttgatcg cgctgtacct gctgactgtc gccgacatcc gcggcacgag ccccaaggtg
    56221 tggaacgcgt ggaaggcaaa gctgctcgag gatctcttct ggttgacgcg caggctgctc
    56281 gcgggcggcg atcgtccgac tcttgccagc agcctgcagg cgcgccagga cgaagcgcga
    56341 gccaagctgc ggctttacgc cgtaccggcg gacgccgaga acaagctatg ggcccagctc
    56401 gatgatgcct atttcctgcg ccacgaaccg caggagatcg cctggcacac gcgactgctc
    56461 tactaccggg tgcagtcgca cgggcccgtg gtcaaggcgc gcctgtcgcc ggccggggaa
    56521 ggcctgcagg tgatgatcta cgtacccgac cagaaagagc tgttcgcgcg catctgcagc
    56581 ttcttcgagc gcatcagcta cgatatcttc gaagccaaga tctacaccac ccgccacggc
    56641 tatgcgctcg acagcttcca ggtgcaggat cccgacaacc gccgcccgca gtaccgtgac
    56701 gtcatcggcc tgatcgagca tgatctggcc gagcgcctgc agcacaagac gccgctgccg
    56761 ccgctctcca agccgcgcct ttcccgccag ttgcggcatt tcccgatctc gcccgaggtc
    56821 aacatccaac ccgacgagaa aggcacttcc tgggtgctgt cagtgatcgc cggcgaccgg
    56881 cccggcttgt tgtcgcggat cgcgcgcgtg ctgaccgcct atgacgtcaa cttgaatacg
    56941 gccaagatca acaccctcgg cgcgcgcgcc gaggacgtgt tcatcatcgc gggcgaggcg
    57001 ctcaaagacc ccaaaacggt cgtgcgtctc gagagcgacc tcgtcgagca gctgcgcacc
    57061 tgacggagtg ctgagttttt aatgttgagt gttaagtgag cccgaattgc ggcccaccac
    57121 ccaaaactca acattcaaca ctcaacacta ttcatcttgc aggtcctgcc cggggaagag
    57181 ctcttcgttg aagccaaaat tacgcaggtc tcggatacgc atggggtaga gaatacccgc
    57241 gagatggtcg cattcatgct gcaccactcg cgcgtgaaag ccgctcacgg tgcggtcgat
    57301 cgctcctccg tactggtcgt agccctggta gcgcaggctc cggtagcgcg gcaccagtcc
    57361 ccgcatgccg ggaacgctga ggcagccttc ccacccttcc tccatttcgt tccccacggg
    57421 cattagcacg gggttgatca gtaccgtgta cggcaccggc tcgacgtcgg ggtaacgggg
    57481 attgtgctcc accccgaaaa tcgccacctg cagtcccatg ccgatctgcg gcgcggcaag
    57541 gcccgcgccg tccaacgccc tcatggtgtc gtccatatcc gcgatcagcg cgtgcagttc
    57601 cggggtgttg aagcgatcga ccggacgcga cggctcgagc agtcgcggat cgcccatgcg
    57661 cagtactgtt ctaaccgtca tcgagcgccg ccagccggtc gaggatgttg cgcacgcgcg
    57721 ccatggcgcg ctgcgagact tcgttgatgt ccgccagacg gaccccctgc gtactggtgc
    57781 cgcggccggc tgcgtaattg acgacgaccg cgatcgccgc gtagcacaag ccgatctcgc
    57841 gcgcgagcgc cgcttccggc atgccggtca tgcccaccat gtcggcgccg tcgcgttcca
    57901 ggcggttgat ctcggccgcc gtctcgagcc tcgggccctg cgtggcggca taggtaccgc
    57961 ccgcgatggc cggctcgctt gccgctgccg tcgccttgag cagccgctga cgcatggctt
    58021 cacaataggg atgggtaaaa tcgatatgcg tcacggcacg gtcggtgcgc gtgaagaagg
    58081 tggaattgcg tccgtgggtg tagtcgatga cctggtccgg gaccgccagc gtgccggggc
    58141 cgaggtccgc gcggatcccg cccaccgagg cgatcgacac cacgtccttc acattctgcg
    58201 catgcaacgc ccagatgttg gctcgatagt tcacctcgtg cggggggatg gtgtggccgt
    58261 atccgtgacg cgccaggaac accacctcgc gtccgccgat cgtgccgaat gtgagcgcgc
    58321 ccgagggctc gccgtacggc gtgcgcacca ccagacgccg cgtcacctcg agattggcga
    58381 gctgggtcag cccgctgccg ccgatgaccg ctagcatagg atcgaggatc gaggatcgag
    58441 gattgagcga aacagaatcg cagatagcgc aatcattgcg acagccaccc ctcaatcttc
    58501 aatcctaaca actccatctt ctttgagggc atagatttcc ggcaggttgc gccacgcccc
    58561 gctgacgtcc atgccgaaac cgaacacgta gcggtcgggc aggcggacgc cgataaaatc
    58621 ggcgctgacg ggcttggcgc ggccggtatt cttttcggcc aacaccgcgc tgaggacacg
    58681 gttggctccg gccccgacga gtttgtcgcg tatcgccgcc agcgtatgcc cctcgtccag
    58741 gatgtcgtcc acgatcagca ccacgcgccc cgccacggac gtgcccggcg tcaccttcca
    58801 ctcgatttca ccgccgtgag tggcttcccg gtagcgggtc agctcgagat agtcgaattc
    58861 gagcgggaac ctgagttgcg gcagcagttg ccccgcgaag accacgctgc cgcgcatcac
    58921 acacaacacg agcgggaagc gctcggccag ggcggccgtg atctcgcgcg ccatgcgtgc
    58981 caccgccccg gacacgtcgg cggcagaaca caacagatcc gccgtgtcca gtacgcgcaa
    59041 ggcttgttcg cgggagagcg ccactcagta ggtgaacgtc acgacccggt cgtccatcgc
    59101 ctgctggatg acgtaggccc cgcccaccgt ttcttcgatc tcgggaatca gatcattgcc
    59161 ccacaggtcc agggcgggcg tgcagacctt gaaatgcacg ccggcctggt gggcgtcctt
    59221 gatgaaatca taaacgctct tgccggagcc ctccttgacg tagagcttct ccgcgacgcc
    59281 cttcttggca agctcccccg cgcgcgccgt caggatcacc tcgacctcgt agtccatcgc
    59341 cgccgccacc gtcgcctgga agaacggcgc accgagttcg gcggtgttgc tggggtcggt
    59401 gttcaccata acaatcatca acttgtcggc catggccatt cctcttgcga ctgcgttctt
    59461 tcccgcctgg tcgcgcgatg aacggcgagg atttcgtccg ttcgatcggg cgcggctcga
    59521 accggcgcct tgcgtccggt ttgagcatga ttataggacc gcggtcggag gcgcgcgcta
    59581 ttttgggagt cggcgtccca agccgcgcag gtaacgcctg aactccatgc cgacttcagg
    59641 atgccggagg gcgtactgga ggtttgcctg cagataaccg agcttgctgc cgcagtcgta
    59701 gcgcgttccg gcaaactcgt gggcgaatac gtcctcctct tgcagcagcg ccgcgatcgc
    59761 gtccgtgagc tggatttcgc cgcccgcgcc gggccggacg cgttgaagat gatggaatac
    59821 ccgcggcgtc aggagatagc gccccacgac acccagcgtc gagggcgcgg ctgcgggttc
    59881 cggtttctcg acgatggcgc tgatgcgatg cggcgacctc gagcgcggat cgagcttgac
    59941 gatgccgtag cggcgcgtct cgtgccgttc cacgttctgc acggcaatga ccgaacgccc
    60001 ccgccgcgcg aacaccgcgc acatttgctt gaggacgggg ggggtgccgt cgatcaggtc
    60061 gtcggcgagc acgaccgcga acggcgcgtc gccgaccacc ggcctggtgc acagaatcgc
    60121 gtgccctagg cccagcgcct gcggctggcg cacgtaagtg cacgaaaccc ggcgcgggag
    60181 gatgccctgg accagtttga gcaactgttt ctgcccgcgg ctctcgagtt cggtctcgag
    60241 ctcgaacgcc ttgtcaaaat ggtcctcgat cgcgcgcttg ccgcggccgg tgacgaaaat
    60301 catctcggtc atgccggctg ccatcgcctc ttccaccgca tactggatca gcggcttgtc
    60361 cacgatcggc atcatttcct tagggcttgc cttggtcgcc ggaagaaacc gcgtgcccat
    60421 ccccgccacg ggaaacaccg ccttgatgat cctgttcatc gttcaggcct tttcacccag
    60481 caatttcagc aatccgtcct cgtcaagcag cgcgatgccg agcgcgcgcg ccttgtcgtg
    60541 cttggatccg gggtcggttc ccacgaccac gtaatcggtt ttttccgaca cgctgcccgc
    60601 gaccttgccg ccgcgcgctt cgatcttttc cttcgcctcc tcgcgcgcga ggtgtggcaa
    60661 ggtgcccgtc agcacgaatg tcttgccggt cagcggcagg ctcgctgccc ctcccccctt
    60721 gccttcgggc cagcgcacgc cgtgcttccg caattgtttc acgacttccc ggttgtgcgg
    60781 ctcccggaaa aaccgcgcga tgctcgccgc aacgaccggc ccgacgtcgg tcacccgctg
    60841 cagtgccgcc tcgtccgcct cgatcagggc atccaggccg ccgaagtgct gcgcgaggtc
    60901 cctggcggtg gcctcgccca cattgcggat acccaacgcg aagatgaacc gggcgagcgt
    60961 ggtgtcccgg cttttagcga tcgccgcgag gaggttcgac gccgattttt cgcccatgtg
    61021 ctcgagtccg gccagcgtcc cggggtcgag ccggtagagg ccggcggggt tttcgacgac
    61081 gccctgatcc accagctggt ccacgagttt ttcgcccagg ccctcgatat ccatcgcgcg
    61141 gcgcgaggcg aaatggagca gcgcctgctt gcgctgagcg ggacagtaca ggcccgccgt
    61201 gcaacgcgca atcgcctcgt cctcgatccg ttccacccgc gacccgcaaa ccgggcattg
    61261 cgcgggcatg tggaacaccc gcaccgcctt cggccggcgc tccctgacca cgccgacgac
    61321 ttccgggatc acgtctccgg cgcgacgcac gacgacatag tcgccgatgt gcacgtcctt
    61381 gcgccgcact tcgtcctcgt tgtgcagcgt ggcgttggtg accgtgacgc cgccgaccgc
    61441 tacgggggcg agacgcgcga ccggcgtcaa cgcgccggtg cgcccaacct gcacgtcgat
    61501 ccccactatc tgagttatct cctcctcggc cgggaatttg tgggcgacgg caaagcgtgg
    61561 cgcccgcgaa acgtacccca gccgatcctg ctggctcagg tcatcgacct tgtagaccac
    61621 gccgtcgatc tggaaaggaa gaccggcgcg atgccgctcg acctcgtggt aatagccgag
    61681 cagcttcttc accccatgca cgacacgccg ttcccggctg accggaaaac ccagcgcctc
    61741 cagataatcc agttggcggc tgtggcggtc gcgcggcacc cgcctgcccg tgaaacggcc
    61801 gaggccgtag gcgaaaaacg tgaggcggcg tgaggcggtc acccgcgagt cgagctggcg
    61861 gagcgatccg gcggcggcgt tgcgaggatt ggcgaattcc cgttcgccct tgccgcgctg
    61921 ctcgcggttc agcgcctcga agtcgcgctt gagcatcatg acctcccccc ggacctccag
    61981 caggccgggg acggattcac cccgcaggcg caacggcagc gcgcgcaccg tacgcaggtt
    62041 ggcggtgacg tcctctccgg tgtagccgtc gccgcgggtt gcgcccatgg cgaatacgcc
    62101 gcgctcatag gtcaggctga ccgccaggcc gtcaaacttg ggttcgaccg catattccac
    62161 ttcgccctcc aggtccaggg cctcccggac gcgccgatcg aaggcttcga cctcctcgat
    62221 gctcaaggcg ttgttgagcg aaagcatggg cgtggcgtgc gtgacctggg cgaatgcggt
    62281 cagcggggcc gtacccaccc gcagcgtcgg tgaatccgga gtggccagag ccgggtggcg
    62341 gctttccaga tcctgcagtt cgcggaacaa gcggtcatac tcggcgtccg gcagggtcgg
    62401 cgcgtcgagc acgtagtaac tgtagttggc gcgctcgatt tcgtcgcgca gccgggcggc
    62461 gcgcgctgtg acgggtttgg ggaccgccat cgttacgaga acagtcgctg cgcgcggttg
    62521 ctgccggccg ggatgccgcg cgccgacatc gtcgcatgaa tcgaacgcac ctgctccttg
    62581 atccgttcga tgccggcttc gctcagggca acgcggttgt catccaccag ccgcccgccc
    62641 aatgccgcgg cgaaacggtt cgcgatctcc agcatgcggt ccaggacctc gacgccgctc
    62701 gccacccgcg gcacgtccag caggagcgtg ataccgcgcg tggacaggcc cttgatgctc
    62761 tccggcagga acggggccgg ttcgtggttg tccagcgtga acagcgtctg ccgctgctcg
    62821 tcgcggtagc ggaacacgcc gtcgggctcg agcttgaagc ccgcggcttc cgcaagcgcc
    62881 cgaatccgcg tgcccgggaa cgttgcacct tccgtcgcga cgacgttgac gccaaccgca
    62941 acatcgacac cggcgcagaa cgcatcgagc tcgcgcgcgc gcttcagggc ggcctgggca
    63001 tcggggcaca ccgccggcat cttgtcagcg cagcttctga ccgcgtcgca aaaagcggcg
    63061 agctgggctg aattgacgat gccggatcgg ctcaccagct gcagcgacaa ccgcagcttg
    63121 cggtagcgcc ctccgctgcc acggacgacg tcttcccatt gctcgttttg gagggcgagc
    63181 ccggcgatac ggacaggctt gccgcaggag gcggtcttgc tcatcaactc gccgatcagc
    63241 gtgtccgata ccggggtacc cgcatcgatt tcggccacgt aatcgagttc atcgtcaaaa
    63301 ccggccacgg aatcggcgtc ccgcgagggg cggacggcgg agcgcccgcc ttctgcacgg
    63361 gccggctctt gcggcgccaa ctgcggctcc agacgaccgt ccgcgagcgt ggactcgact
    63421 ccagccctca acagcacatc gtcatgggcg gccccgaacg cctcacgcat gcggcggtgg
    63481 aagctgcgct cttgcagcca gttgtagaga tagaccgccg cgaccaccac cgcgccgatg
    63541 acaagcaggc tcaactgcag gtcgctcatc gagcaccttc aggccgcggc ttccctcaat
    63601 ttcatcgcct cttcgatgtc aaccgccacc acgcgcgaga cgcccggctc ctgcatggtg
    63661 atgccgagca gctgctcggc gatctccatg gtgatcttgt tgtggctgat gaacaggaac
    63721 tgcgattgct gtgacatctt tttgaccaac tcgcagaagc gttcggtgtt gttgtcatcg
    63781 agtggcgcgt ccacttcgtc gagcaggcag aacggcgcgg gattgagctg gaatatcgag
    63841 aacacgaggg ccagcgccgt gagcgccttc tcgcctcccg acagcaggtg gatggtggtg
    63901 ttcttcttgc cgggcggctg cgcggtcacc tgcacgcccg catccagtat ttcctcgccg
    63961 gtcagcacca gccgcgcatg cccgcccccg aacaacgccg ggaacagctg tccgaagtgg
    64021 cggttgacct catcgaaggt atgctgcaac cgctcgcggg tttcgcggtc gatgcgccgg
    64081 atcgcgtcct cgagcgtcgc catcgcctcg ttgagatcgc gcgattgggc atcgaggtat
    64141 tccttgcggc tgcgcgccgc ttgcagctct tccagcgccg cgaggttgac ggccccgaga
    64201 ctcttgatgt cctcgttcaa ccgggtgatc tcgccttgca gcgcgcccgg ccgcatgccc
    64261 ttctccagtt gctgcgccac ctcctcttcg cgtgccccgg cctcggcgag ctgctgcgta
    64321 tactgctcct cgttgagcct cgcctcctgc tccttcaacc gcatgtcggc gatccgctcg
    64381 cgcaacggct cgagtttctg ctcggcagtg tgacgctcct cgtcggttgc cttgagactg
    64441 ccttccatcc cttccagcgc gtcgcgcgcc cgcgcgagcg cctgctcgcg ctcgctgcgc
    64501 gccgcgaggg cctgttgcaa ccgttgctgc agcggcgcct cgtcatagct ggcgaactcc
    64561 ttccgccgcg agtccagcgc cgtaccgagt cgctgttcgc cttcagagat aactttaatt
    64621 gagttggtta tgtctatgat tttattatta cacgactgtt ggtgaaacac cgcctgctgg
    64681 gccgcttcgc gggcctgctg cagggcattc cgctgcagct ctaggacgtt ttccgcacgt
    64741 cgatacaggt cggtggcaag cgtcaactgc tcgcgcagtc cgctcacttc cgactgcagt
    64801 tcagcgctgc gccgcgccgc ggcctcccca ttggaggtcg cggcggcgga ctcggcctcg
    64861 atttcgacga gctcagacgc gattctttcg gtgcgctgcg tgatcctgtg gtcgagctcg
    64921 gccagctgca aggcctccag ctgaagcgca tgatgacgct gctgcctgtc gttggtctca
    64981 tcgcgcaatt cctcgagccg cgcccgcccc tgctcgacct ccttcgccac cgccgctgtc
    65041 gcctcacgca accggtcgag ctcggcgcgc gctgttccga tggcgccgtc gagttgctcg
    65101 atttcccgct gccgggagag cacgccgtgc aactcgctgt ccggggcatg aaaactcgcc
    65161 gtgtggcgcg tgaatatgtg gccttcccgc gtcaccagca tggcgcccgc cggcagccgc
    65221 tcgcgcagtg caagcccagt tgcggcgtcc gtaatcatat agacctggtg caaccagtcc
    65281 ccgatcaccg cttccaggcg cgcgtcgttg catgtgacat agcgacgcag cggctcgcag
    65341 ccagcgaggg gcgatgcggg cgcagccgcc tcgcgtgcgg gcgcgaacac ggtgatcctc
    65401 cccggtggcg gctcgttgaa ccagcgtcca gactgttcca tgcgctcgag cgcgatggca
    65461 ttcaggcgct gatgaagcac cgcctccagc g
//
LOCUS       DBNGWZ010000370         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_296524, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000370 DBNGWZ010000000
VERSION     DBNGWZ010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_296524"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="AC16CX_10910"
     CDS             complement(<1..154)
                     /locus_tag="AC16CX_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3013054.1"
                     /translation="MNGALFIVSSPSGGGKTSLVKALLEAEPEVRLSVSHTTRPARPG
                     EVNGRDY"
     gene            complement(264..1130)
                     /locus_tag="AC16CX_10915"
     CDS             complement(264..1130)
                     /locus_tag="AC16CX_10915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR3013055.1"
                     /translation="MIHSMTGYATVAREFPFGMLSVELRSVNHRYLDIQFRLPDDLRA
                     IEPALREMLNEHIGRGKVECRVTFSATPGAHKTLKLNENLMLQLEELELKVRTMLAGA
                     GQLSAADVLRWPGVLAAEPLPLEELQAACKELLAAVLREFNAARGREGEKLKMVLLER
                     AAGMERRIAEIIPRLPQVVAAFQERLAARLREALGSGEDERIRQEVAVFATKIDVDEE
                     LSRLSAHVAELRRALANGGTVGKKLDFLMQELNREANTLASKSVDLAVTRAALDLKLL
                     IEQMREQIQNIE"
     gene            1300..>1965
                     /gene="rph"
                     /locus_tag="AC16CX_10920"
     CDS             1300..>1965
                     /gene="rph"
                     /locus_tag="AC16CX_10920"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR3013056.1"
                     /translation="MRPSGRKPDQLRAVRIQRGYTRHAEGSVLIEFGDTRVVCTASVL
                     DRQPPHLKGSQQGWVTAEYGMLPRATNTRGEREAARGKQSGRTQEIQRLIGRALRSVV
                     DLARVGERTIHLDCDVIQADGGTRTAAITGAFVALHDATDFLHQRRLIAASPILDFVA
                     AVSVGVHEGVPVLDLDYAEDVACDTDMNVVMTGSGGLVEVQGTAERAPFSRGEMDAML
                     DLAQ"
BASE COUNT          328 a          638 c          667 g          332 t
ORIGIN      
        1 ggtagtcgcg tccgttgacc tcgcccgggc gcgccggccg cgtggtgtgc gacaccgaca
       61 gcctgacctc gggctcggcc tccagcagcg ccttgaccag gctggtcttg ccgccgccgg
      121 agggggagct gactataaaa agcgcgccat tcatgagagc cgtgactcgt gactcgtgac
      181 tcgtgactcg aacattgttg atgaatgcag cttgaaaatc ggattttcaa ccaggtaacg
      241 agcgacgagc aacgagcaac gagtcactcg atgttctgga tctgctcgcg catctgctcg
      301 atgagcagct tcaagtcgag cgcggcccgg gtcacggcca gatccaccga cttggaggcg
      361 agggtgttcg cctcgcggtt cagctcctgc atcaggaaat cgagcttctt gccgaccgtg
      421 ccgccgttcg cgagcgcgcg tctgagctcc gccacgtgcg cgctgaggcg cgaaagctcc
      481 tcgtccacgt cgatcttggt cgcgaacacc gccacttcct gccggatgcg ctcgtcctcg
      541 ccgctgccca gcgcctccct gaggcgggct gcgagccgtt cctggaatgc ggcgaccacc
      601 tggggaaggc gggggatgat ctccgcgatg cgacgttcca tcccggccgc acgctcgagc
      661 aaaaccattt tcagcttctc gccttcgcgg ccgcgcgcgg cgttgaattc ccgcaggacg
      721 gcggcgagga gttccttgca agccgcctgc aattcctcga gcggcagggg ctcggccgcg
      781 agcacgcccg gccagcgcag cacgtcggct gcgctcagct ggcccgcgcc cgcgagcatg
      841 gtgcgcacct tgagctccag ctcctccaac tgcagcatca ggttctcgtt cagcttgagt
      901 gtcttgtgcg cgccgggcgt ggcggagaag gtcacgcgac attcgacctt gccgcggccg
      961 atgtgctcat tcagcatttc gcgcagagcg ggctcgatcg cgcgcaggtc gtccggcaac
     1021 cggaactgga tgtcgagata gcggtggttg accgagcgca gctcgacgct gagcatgccg
     1081 aatgggaatt cccgcgccac cgtggcgtaa ccggtcatgc tgtgaatcat ggaaaagcgg
     1141 tgaatagtga atggggaatg gggaatggaa gaaaatcagg gcgcgcggcc gcgccgctcc
     1201 cgatccagcc atttcctatt tcccatttcc catttaccat tgacgccgct tcgcagtgcg
     1261 gcggcgtcaa tcggaacgat agcatactca gggagttaca tgcgacccag cggaagaaag
     1321 cccgaccagt tgcgcgcggt gcgcattcag cgcggctaca cgcgccatgc ggagggttcg
     1381 gtgctgatcg agttcggcga cacgcgggtg gtctgcacgg cgagcgtgct cgaccggcag
     1441 ccgccgcact tgaagggctc gcagcagggc tgggtcaccg ccgagtacgg catgctgccg
     1501 cgcgccacca acacgcgcgg cgagcgggag gccgcgcgcg gcaagcagtc cggccgcacc
     1561 caggaaatcc agcggctcat cggtcgcgcc ctgcgctcgg tggtggacct cgcgagagtc
     1621 ggggagcgca cgatccatct cgattgcgac gtgatccagg ccgacggcgg cacgcgcacg
     1681 gcggccatca ccggcgcgtt cgtagcgctg cacgacgcga cggatttcct gcaccagcgg
     1741 cggctgatcg cggcctcgcc gatcctcgat ttcgtggccg cggtctcggt cggcgtccac
     1801 gagggtgtgc cggtgctcga tctcgactac gccgaggacg tcgcgtgcga caccgacatg
     1861 aacgtggtga tgaccgggag cggcgggctg gtggaagtgc agggcacggc ggagcgcgcg
     1921 ccgttctcgc gcggggagat ggacgcgatg ctcgacctcg cgcag
//
LOCUS       DBNGWZ010000371         1724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_797327, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000371 DBNGWZ010000000
VERSION     DBNGWZ010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_797327"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="AC16CX_10925"
     CDS             complement(<1..560)
                     /locus_tag="AC16CX_10925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013057.1"
                     /translation="MKSQEFLETKTKGPVKAISCGTLIDGRGGKPVKDAVVVIQGNRI
                     VKVGEKTRVKAPPGAESIDCSRYTVMPGMMDLHIHTAMYNCMTFHNHRVAQFETMPHL
                     QQMYALFHAQNCFDMGFTTLRDLGMNGPYGLLVNEMCAVRDAINAGIVEGPRMLIAGF
                     TTMTGSHLELIQPRAMVRSGFNTADGP"
     gene            complement(584..>1724)
                     /locus_tag="AC16CX_10930"
     CDS             complement(584..>1724)
                     /locus_tag="AC16CX_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3013058.1"
                     /translation="RSLRLCVQGPSVTDRPPVSIITGFLGSGKTTLLNRLLQDPGMAR
                     AAVIINEFGEIGLDHLLVATPNENTVLLANGCLCCTIRGDLVETLSGLLAQRGAGGIP
                     AFDRVLVETTGLADPVPILRTLASDETITPRLRLGTVITLVDGVNGAGQLDDCPESVK
                     QAAVADCLLVSKIDLAGPAQLRDLRERLFRINPGAQLHEVAHGAIAPERLFEATLDDP
                     AAKREQVERWLNEAAFVKAERGPRLSRRGRGADRHGDNIRAFAVRREGVVTRTGLAAW
                     LDLLAALKGASLLRVKGIVNVDGMPVAVHAVQSVIHEPVALESWPAADRSTRIVFITR
                     DIERAEIEATLPALELDARRAGDGAAIDPEAYARFVRAATGFRAG"
BASE COUNT          263 a          593 c          560 g          308 t
ORIGIN      
        1 gggccgtccg cggtattaaa gccagagcgg accatcgcgc gcggctggat gagttcgaga
       61 tgcgagccgg tcatcgtggt gaagcccgcg atcagcatgc gcggcccttc cacgatgccg
      121 gcattgatgg cgtcgcgcac cgcgcacatt tcgttcacca gcagtccgta gggcccgttc
      181 atgccgaggt cgcgcagcgt ggtaaagccc atgtcgaagc agttctgggc atgaaacagc
      241 gcgtacatct gctgcaggtg cggcatggtc tcgaactgcg cgacccggtg gttgtggaag
      301 gtcatgcagt tgtacatggc ggtgtggatg tgaaggtcca tcattcccgg catcacggtg
      361 tagcgcgagc agtcgatcga ctcggctccc ggcggcgcct tgaccctggt tttttcgcca
      421 accttcacga tgcggttgcc ttgaatcacg accacggcgt ccttcaccgg cttgccgccg
      481 cgtccgtcga tcagcgtgcc gcagctgatc gccttcaccg gccccttcgt cttcgtttcc
      541 agaaattcct gcgacttcac tttccgtttt ctcctttcct gtctcagcct gcgcggaagc
      601 ccgtcgccgc ccgcacgaag cgtgcataag cttccgggtc gatcgccgcg ccatcgcccg
      661 ctcggcgcgc gtccagctcc agcgccggaa gcgtcgcctc gatctccgcc cgctcaatgt
      721 cgcgcgtgat gaacacgatg cgggtgctgc ggtccgccgc aggccacgac tcgagcgcga
      781 ccggctcgtg gatcacgctt tgaacggcgt gtaccgcgac cggcattcca tcgacgttga
      841 cgatgccttt cacgcgcaac aaactggctc ccttgagcgc tgcgagcaga tcgagccacg
      901 ccgccagccc ggtcctcgtc accacgcctt cgcgccgcac cgcaaaggcc cggatgttat
      961 cgccgtggcg atcggcgccg cgtccacgcc ggctgaggcg cggcccgcgc tcggccttga
     1021 cgaacgccgc ctcgttgagc cagcgctcga cttgctcccg cttcgccgcc ggatcgtcga
     1081 gcgtggcttc gaacagccgc tccggcgcga tcgcgccgtg cgcgacctcg tggagctgag
     1141 cgccggggtt gatgcggaag aggcgctcgc gcagatcgcg cagttgcgcc ggccccgcca
     1201 ggtcgatctt gctcaccagc aggcagtccg cgacggccgc ctgcttcacc gactccgggc
     1261 agtcatcgag ctggcctgcg ccgttcactc cgtccaccag cgtgatcacg gtcccgagcc
     1321 gcagccgcgg cgtgatggtc tcgtcggagg cgagggtgcg caggatcggc accgggtcgg
     1381 cgaggccggt ggtctcgacc agcacgcggt cgaacgccgg gatgccgcct gcgccgcgtt
     1441 gcgccagcaa accggacagc gtctcgacaa gatcgccgcg gatcgtgcag cacaggcagc
     1501 cgttggcgag cagcaccgtg ttctcgttgg gggtggcgac gagcaggtga tcgagcccga
     1561 tctcgccgaa ttcgttgatg atgaccgccg cccgcgccat gccggggtcc tggagcaagc
     1621 ggttgagcag cgtcgtcttg ccgctgccca agaagccggt gatgatggat accggcggcc
     1681 ggtctgtcac gctaggacct tggacgcaaa gtcgcaaaga tcgc
//
LOCUS       DBNGWZ010000372         2743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_420494, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000372 DBNGWZ010000000
VERSION     DBNGWZ010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_420494"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16CX_10935"
     CDS             complement(<1..266)
                     /locus_tag="AC16CX_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3013059.1"
                     /translation="MYVIIVGGGRTGSHLASLLLQEGHEVRLIDHRPDILATLHREIP
                     TEVIFEGDGTDPQTLEAAGIQRAQVVAATTADDAENLVVASLAR"
     gene            complement(269..946)
                     /locus_tag="AC16CX_10940"
     CDS             complement(269..946)
                     /locus_tag="AC16CX_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3013060.1"
                     /translation="MGTGTQQQIIIVGCGRVGGELALSVFRKGHSVAVVDSNPRAFDR
                     LGSEFRGRTVQGDGFAEDVLKRAGIETADAVAAVTTSDSVNIIVARVARDLYHIPHVV
                     ARVYNPRRAPIYEKLGLQTIASSSWGAQRIEQLILHPGLQSVYSAGNGEVQIYEVSVP
                     HGWDGGAVGDLVPDVNALPVAIARGGRACLPDRSTILRAHDVLQVSATDEGAAILRQR
                     LQSRGEK"
     gene            complement(1004..>2743)
                     /locus_tag="AC16CX_10945"
     CDS             complement(1004..>2743)
                     /locus_tag="AC16CX_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR3013061.1"
                     /translation="IVLLLVIVTVSYEQTIHAYPGGGGAYIVARDNLGEAPAQTAGAA
                     LLTDYILTVSVSISSGVAQIASAFPALNPFRVEIAVGIVIFMMMVNLRGVKESGITFA
                     IPTYFFLVVAVLTLVVGFFRYLTGSLAPVQGMIPAEASATQALTLFLILRAFASGCTA
                     LTGVEAISNGITAFKEPKSRNAGITLVWMSAILGVMFLGITFLSHEIGAQFSLKETVI
                     SQVGRAIWGVTPQLVDKTGEVTAWNTGAFGFIWYVLLGGTTLILIMAANTSFADFPRL
                     GALHAGDGFLPRQLTYRGSRLVFTYGIVALAAFASLLIIIFRAEVTALIPLYAIGVFL
                     SFTLSQTGMAVRWLRSSRLKEGEEIVQQGGVVRYDPKWRLKLVINGLGAVVTAVVMVV
                     FAVAKFAQGAWVIVLLVPTLVLIFFRIHHHYTDVAKHLSLESFGAPPRIRRNRVLLLI
                     GGVHSGALHALRYARSLSQDVTAVYVAADPAETEKVKGKWEHWGDGMRLVVIDSPYRR
                     LIEPLLDYIDELAAGRQPQEMLTIVVPQFVPTHWWQRFLHMNTAQILQQALLRKSDIV
                     VMEVPYHIGDETH"
BASE COUNT          534 a          854 c          889 g          466 t
ORIGIN      
        1 tggcgcgcca gcgacgcgac gaccagattc tcggcgtcgt ccgccgtggt cgcggcgacg
       61 acctgcgccc gctggatgcc cgccgcttcc agcgtttgcg ggtcggtccc gtcgccttca
      121 aagatgacct cggtggggat ttcgcggtgc agggtggcga ggatgtccgg gcgatggtca
      181 atcaaccgga cctcgtgacc ctcttgcagc aagagtgaag ccagatgaga gccggtgcgg
      241 ccgccgccga cgatgatgac atacatggct atttctcccc tcggctctgg aggcgctggc
      301 gcaaaattgc cgccccttcg tcggtcgcgc tcacctgcag gacgtcgtgg gcgcgcagga
      361 tggtgctgcg gtccggtagg caggcccgcc ccccgcgcgc gatcgccacc ggcagggcat
      421 tcacgtcagg gaccagatcg ccgaccgcgc ccccatccca cccatgtgga acggagacct
      481 cgtagatctg cacttcgccg tttccggcag agtacacgct ctgcaaacct ggatgcagga
      541 tcaattgctc gatgcgttgc gcgccccacg aggaggaggc gatggtttgc aggcccagct
      601 tttcgtagat gggcgcgcgg cgcgggttgt acacgcgggc gacgacgtgc gggatgtgat
      661 agaggtcgcg cgccacgcgc gccacgatga tgttgacgct gtccgaggtc gtgaccgccg
      721 cgacggcgtc agccgtctcg atcccggcgc gcttcagcac gtcttcggcg aagccgtccc
      781 cttgcacggt gcggccccga aactccgatc ctagccggtc gaaagcgcgc gggttggagt
      841 cgaccacggc cacggagtgg cctttgcgga agaccgacag cgccaattcg ccgcccacgc
      901 gcccgcagcc gacgatgatg atttgttgtt gcgtgcctgt tcccatgacg tatcagtcct
      961 tgtcgagaaa ccccggattg gaattgaagg cggctggccg actttagtgc gtttcgtccc
     1021 cgatgtgata gggcacttcc atcaccacga tatcgctctt gcgcagcaac gcctgctgca
     1081 ggatttgagc cgtgttcatg tgcaggaatc tctgccacca gtgcgtgggc acaaattgcg
     1141 gcaccacaat ggtgagcatt tcctgcggct ggcgccccgc cgccagttcg tcaatgtagt
     1201 cgagcagcgg ctcaattagc cgccgatagg gcgagtcaat gacgaccagc cgcatgccgt
     1261 cgccccaatg ctcccatttg cccttgacct tttcagtctc cgccgggtcg gcggccacgt
     1321 acacggccgt gacatcctgc gacagcgagc gggcgtagcg taacgcgtgc agcgccccgc
     1381 tgtgcacacc gccgatgagg agcagcacgc ggttgcgccg gatgcgcggc ggcgcgccga
     1441 aggactccag cgacagatgc ttggctacgt ccgtgtagtg gtggtggatg cggaagaaga
     1501 tcaagaccag cgtgggtacg aggagcacaa tgacccatgc gccttgcgcg aacttggcga
     1561 ctgcgaacac gaccatcacc acggcggtga cgaccgcacc cagaccgttg atgacgagct
     1621 tgagacgcca tttgggatcg tagcgcacca caccgccctg ctggacgatc tcctcacctt
     1681 ccttgagccg gctggagcgc agccagcgca cggccatgcc ggtctgcgac agcgtaaagg
     1741 ataggaacac gccgatggcg tagagcggga tgagcgcggt aacctcagcg cggaagatga
     1801 taatgagcaa cgaggcaaac gccgcgagcg cgacgatccc gtacgtaaac accagccgac
     1861 tgccgcgata cgtcagttgg cgcggcagga agccgtcgcc cgcgtgcagc gcccccagcc
     1921 gggggaaatc ggcgaacgac gtattagccg ccataatcag gatgagtgtc gtcccgccga
     1981 gcaggacgta ccatatgaat ccgaacgcgc cggtgttcca cgccgtgacc tcgccggttt
     2041 tgtccaccag ttgaggggtc acgccccaga tggcgcgccc gacctgcgag ataacggttt
     2101 ccttcagcga gaattgtgcg ccaatctcat gagacaggaa ggtgatgccg agaaacatga
     2161 cgcccagaat cgcgctcatc cacacgagcg tgatgcccgc gttccggctt ttcggttcct
     2221 tgaaggccgt gatgccgttg gagatcgctt ctacgcccgt gagcgcagtg cacccgctgg
     2281 caaaggcgcg caggatcaga aacaaggtca atgcttgagt ggcgctggct tcggccggga
     2341 tcatcccctg cacgggggcg agtgagccag tgagatatct gaagaagcca acgaccagcg
     2401 tgagcactgc gacaacgagg aaaaaatagg tcgggatcgc gaaggtgata ccggattctt
     2461 tcaccccacg caagttgacc atcatcatga agatgacgat gcccacagcg atttccacgc
     2521 ggaaggggtt cagagccgga aaagcggaag cgatctgagc cacgcccgaa gaaatgctca
     2581 ccgagacggt gaggatgtaa tcggtgagga gcgcggcccc ggcggtctgc gcgggggctt
     2641 cgccgaggtt gtcgcgggcg acgatgtacg cgccgccgcc gcccggatag gcgtggatcg
     2701 tctgctcgta cgagacggtg acgatgacca gcaacaggac aat
//
LOCUS       DBNGWZ010000373         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1931513, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000373 DBNGWZ010000000
VERSION     DBNGWZ010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1931513"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..274
                     /locus_tag="AC16CX_10950"
     CDS             38..274
                     /locus_tag="AC16CX_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013062.1"
                     /translation="MNTLSQFLFWVGILSIPLSWVMWYLGPKIEIGRHVAGKIADPAL
                     REALMEAHAERWGIFVATWPATLLVLSYLVSQYK"
     gene            789..>1947
                     /locus_tag="AC16CX_10955"
     CDS             789..>1947
                     /locus_tag="AC16CX_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013063.1"
                     /translation="MKNATENGNTGRRSFLKGTVVAAGAAIAATTKLAQAAESATADK
                     PAAGKAAAEKKLPSETFITNPGSDFMADVLKTIGLQYVAINPAAGFRSLQESIINYLG
                     NRNPEIITCLHEESATHMAQGYAKAAGKPIGVMLHGTVGLQHASMALYNAWVDRVPLM
                     VFAGNGIDAATRRPGTETIHSAQDPAAMVRDFVKWDDQPGSLQHFAESTVRAYKFAMT
                     APMEPVMICLDIDLQEHAIAHPERLRIPKVTRVSQPQGDTAALREAAKLLVAAEHPLI
                     IADRAVQSAQGLKLMVELAEALAAPVVDNGGRMNFPTTHDLECSFIRASLVREADVIL
                     LLEVNDPWGNLNSFSDPYKTYRSITKPGAKVITISMQDVYHKSNYQDFQRYM"
BASE COUNT          417 a          611 c          597 g          322 t
ORIGIN      
        1 ggggggaacc gaggggaagc cgtaaaagga ggaacacatg aacacgctat cgcaattcct
       61 gttctgggtg ggaattcttt cgatcccact atcgtgggtg atgtggtacc tgggtccgaa
      121 aatcgaaatc ggaaggcatg tcgcaggcaa gatcgccgat cctgcgctga gagaagccct
      181 gatggaagct cacgcggaac gttgggggat cttcgtcgcg acctggccgg cgacgcttct
      241 ggtgctgagt tatctcgtca gccagtacaa atagaaacgc ccaggagttt gtcggaacct
      301 ggtccgacaa actcctgata taaaaccacc agcaaaaacg caggcaaaaa ggacgatagc
      361 agtgctaagc gacgccgggg cggcgtcgtt gatggcgttt agtcagcgcc tgcggaaccg
      421 gagcgagacg gcgcccacgg ccaggccaag cgcgaacgtg actccatccc tcatcccttc
      481 cccttctccc gagtggagaa gggaaagctc gagcctcccc tctcagcttg ggagaggggc
      541 gggggtgagg atgggctccg aggccgggtc ctgccacgct gcccgggtcc agcctcgagc
      601 gctgcgcatg cggacagacc acgcgggcga gtttcgcgct cttgatttgc gtcatctgcg
      661 ggatctgctc gatgccgtag acgcagatct caccgctcgg cagcgcgcag aaacggtccg
      721 acagattgac tataatcgtg ccgacaccga tgccagtccc gacgccttcc ccgatccagg
      781 agaccgccat gaagaacgct accgaaaacg gcaatactgg ccgccgctcc ttcctcaagg
      841 gcacagtggt agccgccggc gccgcgattg cggcaacgac caagctcgca caggccgccg
      901 aaagcgcgac cgccgacaag cctgcggctg gcaaggctgc tgcagagaaa aagctcccga
      961 gcgagacctt catcacgaac ccgggctcgg atttcatggc cgacgtgctg aagacgatcg
     1021 ggttgcagta cgtcgcgatc aatcccgccg ccggcttccg cagcctgcag gaatccatca
     1081 tcaattatct cggcaacagg aatcccgaaa tcatcacctg cctgcacgag gaatcagcaa
     1141 cgcacatggc gcagggctac gccaaggcgg cgggcaagcc gattggcgtc atgctccacg
     1201 gtaccgttgg ccttcagcac gcctcgatgg ccctctacaa cgcctgggtg gaccgggtgc
     1261 cgctgatggt gttcgcgggc aacggcatcg acgccgcgac gcgccggccc ggtacggaaa
     1321 cgatccatag cgcgcaggat ccggccgcga tggttcgcga tttcgtcaaa tgggacgatc
     1381 aacccggctc gctgcagcat ttcgcggaat ccacggttcg cgcttacaag ttcgccatga
     1441 ccgcgccaat ggagccggtc atgatctgcc tcgacataga cctgcaggag cacgcgatcg
     1501 cccaccccga acggctgcgc ataccgaagg tcacacgcgt atcccagcct cagggcgata
     1561 ccgccgcact tcgcgaggcg gcaaaactgc tcgtcgcggc ggaacacccg ctcatcatcg
     1621 ccgaccgggc ggtgcagtcg gcgcagggcc tgaaactgat ggtcgagctg gcggaggccc
     1681 tcgccgcgcc ggtcgtggac aacggcggga ggatgaattt tccgacgacg cacgatctcg
     1741 aatgttcctt catacgggcg tcgctggtgc gggaagccga cgtgatcctg ctgctcgaag
     1801 tgaacgaccc gtggggcaat ctgaattcct tcagcgatcc ctacaagacc taccggtcga
     1861 tcaccaagcc gggcgcgaag gtgatcacga tctcgatgca ggacgtgtat cacaagtcga
     1921 attaccagga cttccagcgc tacatgc
//
LOCUS       DBNGWZ010000374         4044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1052559, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000374 DBNGWZ010000000
VERSION     DBNGWZ010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1052559"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="AC16CX_10960"
     CDS             <1..162
                     /locus_tag="AC16CX_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR3013064.1"
                     /translation="KKLLADAKLAVAPGIGFGHYGDDHVRFALIENEARTRQALRGIR
                     DMFKKDGLL"
     gene            276..1586
                     /locus_tag="AC16CX_10965"
     CDS             276..1586
                     /locus_tag="AC16CX_10965"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3013065.1"
                     /translation="MRPINVGLLGIGVVGGGTFKVLTRNQEEIARRAGCAITMKMVAD
                     KELDKARRIVGERAIVTADAYEVVGNPDIDIVVELIGGTTVAKDLILKAIANGKHVVT
                     ANKALVAQHGNEIFAAAQKQDVMVAFEAAVGGGIPIIKSLREGLTANRIEWIAGIING
                     TSNFILSEMRERGASFQDALKEAQQRGYAEADPTFDIEGVDAAHKLTIMAAIGFGIPM
                     QFKSAYTEGISKLTQEDIRYAGELGYRIKLLGIAKRKTKGIELRVHPTLIPERRLIAN
                     VEGVMNAILVKGDAVGQTMYYGAGAGSEPTASAIVADLVDVARMLTADPEHRVPHLAF
                     QPDQLSDIPILPMGEVETRYYLRMRVLDKPGVLADITRILADLAISIEAMVQKEPHEG
                     EDQADIIMLTHLTIEKNVDAAIARIEGLPVVVDKVTRIRLEELN"
     gene            1709..2833
                     /gene="thrC"
                     /locus_tag="AC16CX_10970"
     CDS             1709..2833
                     /gene="thrC"
                     /locus_tag="AC16CX_10970"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3013066.1"
                     /translation="MAHYTGLIDKYRDRLPVAADTRAISLNEGATPLIALRKLPGLVR
                     KKVRIYAKFEGLNPTGSFKDRGMTMAVTRAMEEGAGAIICASTGNTSASAAAYAARAG
                     IACFVLIPEGKIALGKLSQAMMHGSVVLQIRGNFDDGMRLVQEVAKAQPVNIVNSINP
                     YRIQGQKTVAFEVIEALGEPPDYHALPVGNAGNITAHWIGYSEAAGENTKALTIPAEM
                     REYAKPRIAQQRPIMLGYQASGAAPIVRGRPVENPETVATAIRIGNPVSWQQALAAGR
                     ESGGWFAECTDEEILHVQKLLAQTEGIFCEPASAIALAGVLKDLELGKIGEGSTIVCT
                     LTGHGLKDPDVAIAQSAKPMTVDAELKVIEKAIREHLTKT"
     gene            3136..3321
                     /locus_tag="AC16CX_10975"
     CDS             3136..3321
                     /locus_tag="AC16CX_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013067.1"
                     /translation="MSTVKEELTRLIQGQPEDSSREEIVRELAFYLMVERGLADSDAK
                     RVISNEDMARRIRSWQK"
     gene            3309..3602
                     /locus_tag="AC16CX_10980"
     CDS             3309..3602
                     /locus_tag="AC16CX_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3013068.1"
                     /translation="MAEITWTAEAQRWLEDIFEYVASDNPRAAASTVQGIYERAQELA
                     TFPEIGHRYSASSRHVRILLYGHYRIAYLVKDDGNVDVLGVFHGSLDITRYQL"
     gene            3822..>4044
                     /locus_tag="AC16CX_10985"
     CDS             3822..>4044
                     /locus_tag="AC16CX_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3013069.1"
                     /translation="MARLLIISTHGSEDPTRAGLAFFMAKGAVEAGHKPEIILAGDAA
                     VLARKQVADSVLPVGIPPLKELIQFALDNG"
BASE COUNT          867 a         1182 c         1320 g          675 t
ORIGIN      
        1 aaaaagcttc tggcggacgc caagctcgcg gtcgcgcccg gaatcggttt cggacattac
       61 ggcgacgatc atgtacgctt cgcattgatc gagaatgaag cccgcacccg gcaagcattg
      121 cggggaataa gggacatgtt caagaaggac ggactgcttt gaaggcaagg cgaaaggcga
      181 aaggcgaaag tcggaaggga tgcgcaactc cggcgcgatg cgccggtcgg gcccactttc
      241 gcctttcgcc ttccgacttt gtgaggtgga atgaaatgag gccgatcaac gtaggactgc
      301 tggggatcgg ggtcgtgggc gggggcacgt tcaaggtgct gacccgcaac caggaggaga
      361 tcgcgcgccg cgccggctgc gccatcacca tgaagatggt cgccgacaag gagctcgaca
      421 aggcgcggcg catcgtgggc gagcgcgcca tcgtgaccgc ggacgcctac gaggtcgtgg
      481 gcaaccccga catcgacatc gtggtcgagc tgatcggcgg caccaccgtc gcgaaggacc
      541 tgatactcaa ggcgatcgcg aacggcaagc acgtcgtcac cgcgaacaag gcgctggtgg
      601 cgcagcacgg caacgagatc ttcgccgccg cccagaagca ggacgtgatg gtggcgttcg
      661 aggcggcggt gggcggcggc attccgatca tcaagtcgct gcgggagggg ctcaccgcga
      721 accgcatcga gtggatcgcg ggcatcatca acggcaccag caacttcatc ctgtcggaga
      781 tgcgcgagcg gggggcgagc ttccaggacg cgctcaagga ggcgcagcag cggggctacg
      841 ccgaggctga ccccaccttc gacatcgaag gggtggacgc cgcccacaag ctcaccatca
      901 tggcggcgat cggcttcggc atccccatgc agtttaaatc cgcctacacc gagggcatct
      961 cgaagctcac gcaggaggac atccgctacg cgggagagct gggttaccgc atcaagctgc
     1021 tcggcatcgc caagcggaag accaagggga tcgagttgcg ggtgcacccg acgctgatac
     1081 cggaacgccg gctgatcgcc aacgtggaag gggtgatgaa cgcgatcctg gtcaagggcg
     1141 acgcggtggg acagaccatg tactacggcg caggcgcggg gtcggagccg acggcctccg
     1201 cgatcgtcgc ggacctggtg gacgttgcgc gcatgctgac cgccgacccg gagcatcgcg
     1261 tgccgcacct cgcgttccag ccggaccagc tgtccgacat cccgatcctg ccaatgggcg
     1321 aggtcgagac gcgctactac ctgcgcatgc gcgtgctcga caagccgggg gtgctggcgg
     1381 acatcacgcg catcctcgcg gaccttgcga tctcgatcga ggccatggtg cagaaggagc
     1441 cgcacgaagg cgaggaccag gcggacatca tcatgctcac ccatctcacc atcgagaaga
     1501 acgtcgacgc ggcgatcgcc aggatcgagg ggctgccggt ggtggtggac aaggtgaccc
     1561 gcatccggct tgaagaactg aattagacgc aaaggcgcga aggcgcgaag acgcgaagaa
     1621 aaacaagaat ggtccagaaa gaattcgccg tcagcggttc taattttcgt attcctttgc
     1681 gtctttgcga ttttgcgtcg agattttgat ggctcattac actgggttga tcgacaagta
     1741 tcgcgaccgg ttgccggtcg cggcggacac gcgcgcgatc tcgctcaacg agggcgcaac
     1801 cccgctcatc gcgttgcgga agctgccggg cctggtgcgg aagaaggtcc ggatctacgc
     1861 caaattcgag gggctcaacc ccaccggctc gttcaaggac cgaggcatga cgatggcggt
     1921 cacgcgggcg atggaggagg gcgccggcgc gatcatctgc gcttccaccg gcaatacctc
     1981 ggcctccgcc gccgcctacg ccgcgcgcgc cggcatcgcc tgcttcgtgc tgatcccgga
     2041 gggcaagatc gcgctgggaa agctctcgca ggcgatgatg cacggatcgg tggtgctgca
     2101 gatccgcggc aatttcgacg acggcatgcg gctggtgcag gaggtcgcga aggcgcagcc
     2161 ggtcaacatc gtcaattcca tcaatcccta ccgcatccag ggccagaaga ccgtcgcgtt
     2221 cgaggtgatc gaggcgctgg gcgagccgcc ggactaccat gcgctgccgg tcggcaatgc
     2281 cggcaacatc accgcgcact ggatcggcta ctcggaggcg gccggcgaga acacaaaggc
     2341 gttgacgata ccggcggaga tgcgcgagta cgcgaagcct cgcattgcgc agcagcgccc
     2401 catcatgctg ggctaccagg cttcaggagc ggcgccaatc gtgcgggggc ggccggtgga
     2461 gaatccggaa acggtagcga ccgccatccg catcggcaac ccggtatcgt ggcagcaggc
     2521 gctcgcggca ggacgggagt ccggcggctg gtttgcggag tgcaccgatg aagagatcct
     2581 tcacgtgcag aagttgctcg cgcaaacgga gggcatcttc tgcgagccgg cttcggcgat
     2641 cgcgctcgcc ggcgtgctga aggacctgga gctcggcaag atcggcgaag gcagcaccat
     2701 cgtctgcacg ctgaccggtc atgggctgaa ggaccccgat gtcgcgatcg cgcagagcgc
     2761 gaagccgatg acggtcgatg ctgaactgaa ggtgatagaa aaggcaatac gcgaacacct
     2821 gacgaaaacc tgaatcgccg agagaagcaa gattgattat tgaaccgcca agacgccaag
     2881 agaatcgata attgaaccgc agagataggg agagaaacaa actaaaattg aaccgccaag
     2941 cccgccaagt aaagttaaat ccggacgctc cgcgccggtt taactccgcg tgttcgaacg
     3001 gacgagatca tcgccgctca acacgcggtt acgagtgaag aatattagcg ggcgcgaaat
     3061 cgagcctgtc ttgctttctg gcggcaaatg ccgggcgcct gtggcagaat tagcggtgac
     3121 acggaggttt tacccatgtc cacagtaaag gaagagctta ctcgccttat tcaggggcag
     3181 cctgaggaca gttcgcgaga agaaatcgtg cgcgagcttg ccttttacct catggtggag
     3241 cgcgggctcg ccgattcgga cgcgaagcgc gttatctcga acgaggacat ggcgcggcgc
     3301 atccgctcgt ggcagaaata acgtggacgg cggaagctca gcgttggctc gaagacattt
     3361 tcgagtacgt cgcttcggat aaccctcgcg ccgcagccag caccgttcaa ggcatttacg
     3421 aacgagcgca agaacttgcc acgtttcccg agatcggcca tcgatattcg gcatcctctc
     3481 gccacgtacg cattcttctc tacgggcact accggattgc atacctcgtc aaggatgatg
     3541 gaaacgttga cgtcctcggt gtctttcacg gttcgcttga catcaccagg tatcagcttt
     3601 gagcagcaaa caaaaacatc cagtaaggca gcagcttgac ggtgtccggg tcgggagaag
     3661 attatttgta tcctgcgagt tgcttcgcgc caatcgagct tctaccgccg gtccgtaaag
     3721 ctgtgctggc ttccgtctag ccgcggtttc gagaacggac gcgccaggaa gcggcgcagc
     3781 gttgaacgcc gacattatgc cgattgaaaa ggaggtgctt catggcacgt ttactcatca
     3841 ttagcacgca tggttccgaa gaccccaccc gcgcaggcct ggcattcttc atggccaagg
     3901 gcgcggtgga ggccggacac aagcctgaaa taatcctggc gggcgatgcc gccgtgttgg
     3961 cgcgcaagca agtcgccgat tccgtattgc cggttgggat tccgccgctc aaggagctga
     4021 tccagtttgc tctcgacaac ggcg
//
LOCUS       DBNGWZ010000375         6674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2815409, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000375 DBNGWZ010000000
VERSION     DBNGWZ010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2815409"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="AC16CX_10990"
     CDS             <1..590
                     /locus_tag="AC16CX_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3013070.1"
                     /translation="IGGNHLVHAMRRNIGLTAIVVNNSVYGLTGGQSSPTTPVGGRTT
                     TARPGSFERPMNLEVLARAAGAAFFARATVNHTAGLTKMIERALGVSGFAMVEVISNC
                     HVLYGKMNELGDAAEMMKSMDRSTRRINPVLLRRAERPLRLTKPDAPSWSAEAAPLEG
                     VAEDDRTPRGVIFERKGSVDFGRRYYAMLDEIKAR"
     gene            670..921
                     /locus_tag="AC16CX_10995"
     CDS             670..921
                     /locus_tag="AC16CX_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3013071.1"
                     /translation="MAQIVRGIYQINVYKELCKACGLCVAWCPQDVLAADAEGYPITP
                     HLSQCINCKLCERHCPDFAIEIIPPEVEVADVQSILHAR"
     gene            893..2014
                     /locus_tag="AC16CX_11000"
     CDS             893..2014
                     /locus_tag="AC16CX_11000"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3013072.1"
                     /translation="MSKASFMHGNEAAAEGAIAAGCRFYAGYPITPSSEIAEILSGRL
                     PEVGGVFIQMEDEIASMAAIIGASLGGLKSATATSGPGFSLMQENLGFAVISEIPCVI
                     INVQRVGPSTGLPTKAAQGDVMQARWGTHGDHPIIALTPSSAQEAFTLTVRAFNLSEQ
                     FRTPVILLMDAVVAQLKEKVETPDPDELTIVHRITPTVPPKEYKPFAFKRGEAAPLAV
                     FGYGYRYHVTGLLHDEYGYPTEAPEVVSQWWEHMTTKIGDHLDEVLQWEEVQLDDAEI
                     VLVAYGGTARAASHAVTLARERGVKLGLLRPITLWPFPEAAVRRVAERVKRIVVPEMN
                     LGQIRFEVERLSAGKAVVEGVNRADGLQITPEQILEKVM"
     gene            2011..3258
                     /locus_tag="AC16CX_11005"
     CDS             2011..3258
                     /locus_tag="AC16CX_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3013073.1"
                     /translation="MTYDRAFATTWQLLLDAICTLMGLGQDRDPPIVLSSLPDLPYPV
                     RGRAPLSFIQGPPGRNIALAIGLRAALPNTPLILIMNADSVTLGTNHLIHAARRNIGM
                     TLLLLRSEVTAAADGDSLDRMRWGLPSYQQTIETAPTPLDWVSALDAAFVGRASLHDP
                     DHLAALIHEAVEASGFSVIGVTAEAGLQLGMLSRTEWPEYFAAYREWARPFQQVGETV
                     FGKGKPGLHQSSRSVPRCEVRIAGFGGQGVKLAGTVLSEAAGLHEGLWATQRGDYGSA
                     TRGGPSMVDVVIGSDPITYAGADHPDVLVVLSRGAAERYARAMKPNARLVADPGEVGA
                     LPPGAIPVPVTALAREHTGKPIAAGMVSLGCVAALSEAVSLDSLRQSIAEHVPRAAVA
                     KNLAAMEAGYAATRRALEGGTHE"
     gene            3251..4537
                     /locus_tag="AC16CX_11010"
     CDS             3251..4537
                     /locus_tag="AC16CX_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3013074.1"
                     /translation="MSRVVIVGGGWAGCSAALTARMGGADEVIVLERTDSLLGTGLVG
                     GIMRNNGRYTATEEMIALGAGQLFEICDATSRHRNVDFPGHQHASLYDVAMIEPAVRK
                     CLLEHGVEIWLMSRVAAIERSNGSIRAVALENGRAIPGDVFVDTTGTVGSQSFCTEHG
                     NGCVMCIARCPTFGPRVSLTGLAGIPEKEGKKADGTIGAMSGSCKLNKDTIAPHLMRQ
                     LEETGLAVIAMPQHLIHQEKLGAKACQQYALREFADNVILLDTGQVKFMAPYMPLDQL
                     RQVPGLENVRYEDPYSGTVGNSMRYSALSPRDDAMRVLGPVDNLFCGGEKAGLLVGHT
                     EAMVTGALAGHNAVRAVAGQELLTLPDSLAVGDAISHVRTEMETEAGLQKKYTFSGSV
                     YFERMKARGLYTTDHAAIAARVASAGLTNIYGQKVL"
     gene            4540..4827
                     /locus_tag="AC16CX_11015"
     CDS             4540..4827
                     /locus_tag="AC16CX_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3013075.1"
                     /translation="MSYMITAECINCSACEMDCPVRAISPAASQYVIDPQVCVECEGY
                     FDEPRCKHVCPVNACVPARETYLFKAKALTHRGAPPLIYKPTALPEVDYGR"
     gene            4817..5029
                     /locus_tag="AC16CX_11020"
     CDS             4817..5029
                     /locus_tag="AC16CX_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013076.1"
                     /translation="MADEREPQRLEELRRRLDALQKKLEIVTNKETRAEVRYEIAKIQ
                     WQLGLISDEEFHQIEEFYESFTYEWC"
     gene            5113..5640
                     /locus_tag="AC16CX_11025"
     CDS             5113..5640
                     /locus_tag="AC16CX_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3013077.1"
                     /translation="MMPIIKGRVWKYGDNVNTDVIFPGKYTYTVSKPSETAQYALEDL
                     DPRFAKEVQPGDVIVGGKNWGNGSSREQAVTCLKESRVGAIIAKSFARIYSRNCINMA
                     LPAITCPEAVDAIQPGEVVEVDMEAGEIRCAAGTFKFAPLPPSVLGIFAAGGLVPYTR
                     KKLEAMKAAKAAETK"
     gene            5684..>6674
                     /locus_tag="AC16CX_11030"
     CDS             5684..>6674
                     /locus_tag="AC16CX_11030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012407.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3013078.1"
                     /translation="MTKYKIAWMPGDGIGVDVMDAARIVLDKLRLDAEYVHADIGWEF
                     WCKEGDALPRRTIDTLKNTTCALFGAITSKPKDEAERELIPELRGKGLSYRSPIVRLR
                     QMFDLYCNLRPCKAYPGNPLNYRGDIDLVVFRENTEGLYAGVEFRPLPDEVRATLLKH
                     SSAMSRFKDTPANDIAISARIITRRGAHRIVRSAFDYALGNGYKSVTLVEKPNILRET
                     SGLMVEAAREVAKDYPDIQLWDTNIDAQVMWLIKNPKDYGVLVTSNMFGDILSDLCAQ
                     LVGGLGFASSGNIGDEYAVFEPTHGSAPKYAGQHKVNPMAMLLAAKLMLDWLGE"
BASE COUNT         1278 a         2061 c         2226 g         1109 t
ORIGIN      
        1 ccatcggcgg caatcatctg gtgcacgcca tgcgccgcaa catcggcctg accgccatcg
       61 tcgtcaacaa cagcgtctac ggcctcacgg gcggccagtc gtcgccgacc acacccgtcg
      121 gcgggcggac gacgacggcg cggcccggct ccttcgagcg cccgatgaat ctcgaagtcc
      181 tggcgcgcgc ggcgggcgca gcgtttttcg cccgcgcgac cgtcaaccac accgccgggc
      241 tgacgaaaat gatcgagcgc gcgctgggcg tgtccggatt cgcgatggtc gaggtcatca
      301 gcaactgcca cgtcctttac gggaagatga atgaactggg ggacgccgcc gagatgatga
      361 aaagcatgga tcgcagcacg cgccggatca atccggtgct gctgcgccgc gccgagcggc
      421 cgctgcgact gacgaaacca gatgcgccct cgtggtcagc ggaggcggcc ccacttgagg
      481 gcgtcgccga ggacgaccgc accccgcgcg gcgtgatctt cgagcgtaag ggttcggtgg
      541 atttcggccg gcgctattac gcgatgctgg acgagatcaa ggcacggtga cggttccggg
      601 aatccgcaga tttcgcagac ggcacagatc gttatcctcg gcgaaatctg cgccatctgc
      661 ggataaaaca tggcacagat cgttcgcggt atctatcaaa tcaacgtcta caaagagcta
      721 tgcaaagcct gcgggctgtg cgtggcgtgg tgtccgcagg acgtactcgc ggcggatgcg
      781 gaagggtatc cgatcacgcc ccatctgtcg cagtgcatca actgcaagct gtgcgagcgg
      841 cactgccccg atttcgcgat tgaaatcatc ccgccggagg ttgaggtagc cgatgtccaa
      901 agcatccttc atgcacggta acgaggcggc ggcggagggc gccatcgcgg cgggctgccg
      961 cttttacgcc ggatacccga tcacgccctc gagcgaaatc gccgaaatcc tctcgggccg
     1021 cctgcccgag gtgggcggcg tgttcatcca gatggaagac gagatcgcca gcatggccgc
     1081 catcatcggc gcatcgctcg gcggactgaa gagcgccacg gccaccagcg gccccggctt
     1141 ttcgctcatg caggagaacc tcggcttcgc ggtgataagc gagattccgt gcgtgatcat
     1201 caacgtccag cgcgtggggc cgagcaccgg cctgcccacc aaagccgcgc agggcgacgt
     1261 gatgcaggcg cgttggggca cgcacggcga ccatcccatc atcgccctca cgccgagcag
     1321 cgcgcaggag gcgttcacgc tcacggtgcg cgccttcaat ctctcggagc agtttcgcac
     1381 acccgtcatc ctgctcatgg acgccgtcgt ggcgcaactc aaggagaaag tggaaacccc
     1441 cgatccggat gaactcacaa tcgtccaccg catcaccccg accgttccgc cgaaggagta
     1501 caagccgttc gccttcaagc gcggtgaggc cgcgccactc gccgtgttcg ggtacggcta
     1561 tcgctatcac gtcaccggcc tgctgcacga cgagtacggg tatccgaccg aggcccccga
     1621 ggtcgtcagc cagtggtggg aacatatgac caccaaaatc ggcgatcacc tcgacgaggt
     1681 tttgcaatgg gaggaggtgc aactcgacga cgccgagatc gtgctcgtgg cctatggagg
     1741 aaccgcgcgc gccgcttccc atgcggtgac gctcgcccgc gagcgcggcg tcaaattggg
     1801 gctgcttcgg cccatcaccc tgtggccttt cccggaggcc gccgtgcgcc gagtggcgga
     1861 gcgtgtgaag cgcatcgtcg tccccgaaat gaatctcggc cagattcgat tcgaggtcga
     1921 acgcctcagc gcgggcaagg ccgtggtgga gggcgtgaac cgcgcggatg gattgcagat
     1981 cacgccggag caaatcctgg agaaggtgat gtgacttacg accgggcttt tgcgacgacg
     2041 tggcagttgc tgctggacgc catctgcacc ttaatggggc tgggccagga ccgcgatccg
     2101 cccatcgtcc tctccagcct gccggacttg ccctatccgg tgaggggccg tgcaccgctg
     2161 tcgttcattc aggggccgcc gggccggaac attgcgctcg ccatcgggct gcgcgcggca
     2221 ttgccgaata cgccgctgat cttgatcatg aacgccgaca gcgtgacgct ggggacaaat
     2281 cacctgatcc atgccgcgcg gcggaatatc ggcatgacgt tgctgctgct gcggtcggag
     2341 gtcacggcgg cagcggatgg ggattcgctc gaccggatgc ggtgggggtt gccctcgtat
     2401 caacagacga tcgaaaccgc gccgacgccg ctggattggg tctcggcgct tgacgcggct
     2461 ttcgtcgggc gggcctcgct gcacgacccc gatcacctgg cggcgctaat tcacgaggcc
     2521 gtggaagcgt ccggcttctc cgtcatcggc gtcacggctg aagccggttt acaactcggg
     2581 atgttgagcc gcaccgagtg gccggaatac ttcgccgcgt atcgtgagtg ggcgaggccg
     2641 tttcagcagg tgggcgagac ggttttcggg aaaggaaaac ccgggctgca ccaatcatcc
     2701 cggagtgtac cgcgctgcga ggtgcgcatc gccgggttcg gcgggcaggg cgtcaaactg
     2761 gcgggcacgg tgctgagcga ggccgccggg ctgcatgagg gattgtgggc cacacagcgc
     2821 ggcgactacg gttcggcgac gcgcggcggg ccgagcatgg tagatgtggt gattgggagt
     2881 gacccgatca cgtatgcggg cgcggatcac cccgacgtgc tggtcgtgtt gagccggggc
     2941 gcggcggagc gttacgcgag agcgatgaaa ccgaatgcgc ggctggttgc cgatccgggc
     3001 gaggtgggcg cgctgccgcc gggcgcgatc cccgtgcccg tcaccgcgtt ggcgcgcgaa
     3061 cacaccggca agccgattgc ggcggggatg gtgtcgctcg gctgcgtcgc ggcgctgtcg
     3121 gaggcggttt cgctcgattc gttgcggcag agcatcgccg agcacgtgcc gcgcgcggcg
     3181 gtcgctaaaa atctggcggc gatggaagcg gggtatgcgg cgacgcgacg cgcattggaa
     3241 ggaggcacac atgagtaggg ttgtgattgt gggcggcggg tgggccgggt gctcggcggc
     3301 gctgacggcg cgcatgggcg gtgcagacga agtcatcgta ttggaacgca ccgacagcct
     3361 gctcggcacg gggctggtcg gcggcatcat gcgcaacaac gggcgctaca cggccaccga
     3421 ggaaatgatt gcgctcggcg cgggccagct cttcgagata tgcgacgcga cctcccgcca
     3481 ccgcaacgtg gatttccccg gccaccagca cgcgagcctg tacgacgtgg cgatgatcga
     3541 accggcggtg cgcaaatgcc tgcttgagca cggagtcgaa atctggctga tgagccgggt
     3601 ggcggccatc gagcgcagca acggctctat ccgtgcggtc gcgctggaaa acgggagggc
     3661 gatcccgggt gacgtattcg ttgacacgac cggcacggtc gggtcacaaa gtttctgcac
     3721 ggagcacggg aacggctgcg tgatgtgcat cgcgcgctgc ccgaccttcg gcccgcgtgt
     3781 gagcctgacc gggctggccg gtatcccgga gaaggagggc aagaaagccg acgggacgat
     3841 tggcgcgatg agtggctctt gcaaattgaa caaggacacc atcgcgccgc acctcatgcg
     3901 ccagttggag gagactggcc tcgccgtcat cgccatgccg cagcatctga tccaccagga
     3961 aaaactgggc gccaaggcct gccagcagta cgcgctccgc gagttcgccg acaacgtgat
     4021 tctgctcgac accggacagg tcaagttcat ggccccgtac atgccgctcg atcagctgcg
     4081 gcaggttccg gggctggaga acgtgcgcta cgaagacccg tactccggca cggtcggcaa
     4141 ctcgatgcgg tactcggcgc tctcgccgcg cgacgacgcg atgcgcgtgc tcggcccggt
     4201 ggacaatctg ttctgcggtg gcgagaaggc cgggctgctg gtcggccaca ccgaggcgat
     4261 ggtgacgggc gcgctcgccg ggcacaacgc ggtgcgggcg gtggcgggcc aggaactgct
     4321 caccctgccc gactcgctcg ccgtgggcga tgcgatcagc cacgtgcgca cagagatgga
     4381 aacggaagcg ggcctgcaaa agaagtacac cttctccggc tcggtctatt tcgagcgcat
     4441 gaaagcgcgc ggcctgtata ccaccgatca cgccgccatc gccgcgcggg tagcctccgc
     4501 tgggttgacg aacatttacg gccagaaggt cctctgacga tgtcatacat gatcacggcg
     4561 gagtgcatca attgcagcgc gtgcgagatg gattgcccgg tgcgcgccat ttctccggcg
     4621 gcgagccagt acgtgattga cccgcaggta tgcgtcgaat gcgagggcta tttcgacgag
     4681 ccgcgctgca agcacgtctg cccggtgaac gcctgcgtcc cggcgcgtga gacgtacttg
     4741 ttcaaggcca aagccctgac gcaccggggc gcgccgccgc tgatctataa gccaaccgct
     4801 ttgccggagg tggactatgg ccgatgagcg tgagccacag cgcctcgagg aacttcgccg
     4861 ccgcctcgac gctttgcaga agaaactgga gatcgtcacc aacaaagaga cgcgggccga
     4921 agtgcgttac gagattgcga agatccagtg gcagctcggc ctcatcagcg atgaggagtt
     4981 tcaccagatc gaggagttct acgagagttt cacttacgag tggtgctgag ggatgtgcga
     5041 cgcactgctc atctcctccg ctacgctgcg ggggcctgcg gagtgcgtcg cacgtaaagg
     5101 aaggaggtca gcatgatgcc catcatcaaa ggccgcgtgt ggaagtacgg cgacaacgtc
     5161 aataccgacg tgatctttcc aggcaaatac acatataccg tcagcaagcc gtcggagacg
     5221 gcgcaatatg cgctcgaaga cctcgacccg cgcttcgcca aagaagtcca gccgggcgac
     5281 gtcattgtcg gcggcaagaa ctggggcaat ggctcgtcgc gcgagcaggc ggtgacgtgt
     5341 ttgaaggaat cgagagtcgg agccatcatc gccaaatcgt tcgcgcgcat ctactcgcgt
     5401 aattgcatca acatggcgct tcccgccatc acctgcccgg aggccgtgga cgcaatccag
     5461 ccgggtgagg tcgtggaggt ggatatggaa gcaggcgaaa tccgctgcgc cgccgggacg
     5521 ttcaagttcg ctcctctgcc cccgagcgtg ttgggcatct tcgcggcagg tgggctggtt
     5581 ccgtacacgc gcaagaaact ggaagcgatg aaggcggcga aggcggctga gacgaaatga
     5641 tgaccttgtc agggaatacc caagcacttg gagaataaag acgatgacca aatacaaaat
     5701 cgcctggatg cccggtgacg ggatcggggt ggacgtgatg gatgcggcgc gcatcgtgct
     5761 cgacaagctc aggctcgacg ccgagtacgt tcacgccgac atcggctggg aattctggtg
     5821 caaggaaggc gatgcgctcc cccgacgcac gattgatacc ctcaagaaca ccacctgcgc
     5881 gctgttcggc gcgatcacct ccaaaccgaa agacgaagcc gaacgcgaac tcatcccgga
     5941 actacgcggc aagggcctct cgtatcgtag ccccatcgtc cgcctgcggc agatgttcga
     6001 cctgtattgc aacctgcgcc catgcaaggc ttatccgggc aatccactca attatcgcgg
     6061 cgacattgac ctcgtcgtct tccgcgagaa cacggagggg ctatatgcgg gcgtcgagtt
     6121 tcgcccactg ccggacgaag tgcgcgcaac gctcctcaag cacagttcgg cgatgagccg
     6181 cttcaaggac acgcccgcca acgacatcgc catctccgcc cgcatcatca cccgccgcgg
     6241 cgcgcaccgc atcgtgcgca gcgcgttcga ctatgccctg ggcaacggtt acaagtcggt
     6301 gacgctggtg gagaagccca acatcctgcg cgagacgagc ggtttgatgg tggaggccgc
     6361 gcgcgaagtc gccaaagact accccgacat ccaactgtgg gacacgaaca ttgacgcgca
     6421 ggtgatgtgg ctgatcaaaa acccgaagga ctacggcgtg ctcgtcacca gcaatatgtt
     6481 cggcgacatc ctctccgacc tgtgcgcgca gctggtgggc ggactgggtt tcgcctccag
     6541 cggcaacatc ggcgacgaat atgcggtctt tgagccaacg cacggctcag ccccgaaata
     6601 cgccgggcaa cacaaggtca atccgatggc gatgctgctc gccgcgaaac tgatgctcga
     6661 ttggctgggc gaag
//
LOCUS       DBNGWZ010000376         6225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2563064, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000376 DBNGWZ010000000
VERSION     DBNGWZ010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2563064"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="AC16CX_11035"
     CDS             <1..722
                     /locus_tag="AC16CX_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013079.1"
                     /translation="VIKDFAGAGVEALLVALDLETTLGTPPCSNAYVHAMWKRHPERI
                     IQAWGAIDPAKGEAAITEARKAVRELGMIGFHFHPIMQHFAVNDRRCYPLFEAINELK
                     AAVMIDIGTTGMGAGMPGGMGARIRHAHPSAIDDLAADFPDLKIIMAHPGWPWVDEAT
                     AVALHKGNVYWEMSGWAPKHFPAQLKVDMRARLQDKVLFGSDYPSMPYARILGEWQEL
                     GYGDAVMEKIFHRNAERILGL"
     gene            complement(1053..2024)
                     /locus_tag="AC16CX_11040"
     CDS             complement(1053..2024)
                     /locus_tag="AC16CX_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013080.1"
                     /translation="MARSALRMLLLCLALACPALALGQAYPARPIRLVVPYTPGTGID
                     IIARLVGPKLNERWNQPVIVDNRPGASGNLGADIVAKSQPDGHTLMVTVNTFTITPAL
                     YKDLPYDPLRDFAPIGKAATGSLALVVNSTALPVKKLDELTAAARARPGKLSYSSPGN
                     GTPQHLAVELLKQRLGLDVLHVPYKGAAGALADLLGGQVQLAVLPVHTALPHAKAGRL
                     NVIAVSGESRSVLAPDSPSFAESGLKNLDIDLYFWFAGPTGIPRDVVLKVNRELAVAL
                     ALSDVRESLLKQGLVPQTSSPEAIAAQIRADVARWKKFIAAAKITAD"
     gene            complement(2039..3130)
                     /locus_tag="AC16CX_11045"
     CDS             complement(2039..3130)
                     /locus_tag="AC16CX_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013081.1"
                     /translation="MACAGRAPARDAAFAANMKIDLYNHVMPARYLEKVKQHSKEPGM
                     VKRMSSLRMLWDIESRVTMLDQWPDLQQVLTLSLPAPELLGGPELSPELARIANDGMA
                     EMCARWPKKFPAFVASLPMNNVPAALEEMDRAVARLGARGVQILTSVNGRPLDDPEFL
                     PVFERATRKHDLPIWMHPARPASRPDYVNETGSKYEIWQVLGWPYETSVAMARIVFSG
                     LFEALPDMRIVTHHCGAMIPFFAGRCETLWAQLGSRSVDENYEELLRRMAKKPIEYFR
                     MFYADTVLGGSASALRCGLDFFGADRVVFASDCPFDPEGGPMFIREGMRSVEDLKLPE
                     SDKRKIYFENAMRLLRMAVASGKQAGPAP"
     gene            3292..3831
                     /locus_tag="AC16CX_11050"
     CDS             3292..3831
                     /locus_tag="AC16CX_11050"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3580..3582,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3013082.1"
                     /translation="MSRKVVLVVPEALVPESAVTAESRKAGGSGLRLGVLDNSKGNAD
                     HLLKFIVEGVKAAVPVASVVSLRKASVSLPAPGAILDQLTVEADFVVSAMADUGSCTS
                     WSVHDMCELRKRGLVTAVICSEPFLKLGKTQARVFGVPDLPLLLIPHPLGGLSLEQVE
                     GRAAHAIPKVLKLIGKHFK"
     gene            3828..4943
                     /locus_tag="AC16CX_11055"
     CDS             3828..4943
                     /locus_tag="AC16CX_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013083.1"
                     /translation="MTDLADFLRAPGASLECEDDFDTVNELVRARGWSDGLPVVPPTA
                     QRVERMLAYCDRPWGEPVAKVAPRYGEATPLRLAANAVMAGCRPEYFPLLVLAIEAMC
                     EEPFNLYGVQATTHLCAPLVIVNGPVAKELDINSGHNAFGPGWRANATIGRAIRLALV
                     NIGGAIPALGDMSTFGAPSKFSYLAAENEEASPWEPLHVERGLPREASAVTVIGAECP
                     HNVNDHESISAEGILTTIAGTMLGTGANDVYYEAQPLIVMGPEHAKTVAGGGYSKADA
                     KRFLHEHAHLPLGRFSRENIERRLRVTWKERYANAGPDAPVYMVQRPEDILIAVIGGA
                     GKHSAVIQTFGATKAVTRALKTRDGSYARSIADFRKK"
     gene            5061..6035
                     /locus_tag="AC16CX_11060"
     CDS             5061..6035
                     /locus_tag="AC16CX_11060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR3013084.1"
                     /translation="MKCYYIKSVNGRTVFEPREAPKPQPKAGEMLVRVRAASLNRGEI
                     LASISLHQVHEPHPAGGDCAGEVEAVGEGVTAFKPGDAILGRARGSFAEYVVMGAAQA
                     AAKPQRLTWEQAAAVPIVSVTAYEAICQYGKLKSGETLLVAGAASGVGVIAVQAGKYL
                     GAKVIGISRSNDKLEKLKVIGLDVGIQAAGGGFADKVLEATGGKGVNLALNLIGGSAF
                     PDLVRSLANQGRLAIIGYVDGQHHAEIDLETVHGKRLQIFGVSNALMTAPQRAEAMRG
                     FARDLLPGFAEGRITPVVDKVFPFNELPAAKAYVETNAQLGKVVVTVP"
BASE COUNT         1081 a         2029 c         2098 g         1017 t
ORIGIN      
        1 aggtcatcaa ggacttcgcc ggcgcggggg tcgaagcgct tctggttgcg ctcgatctcg
       61 agacgaccct gggcacgccg ccgtgctcca acgcctatgt tcacgcgatg tggaagcgcc
      121 atccggaacg catcatccag gcgtggggcg cgatcgaccc ggcgaagggc gaagccgcga
      181 tcaccgaggc gcggaaagcg gtgcgggaac tcgggatgat cggcttccac tttcatccga
      241 tcatgcagca tttcgcagtc aacgaccggc gctgctatcc cctgttcgag gcgatcaacg
      301 agctgaaggc cgccgtgatg atcgacatcg gcacgaccgg catgggagcg gggatgcccg
      361 gcggcatggg cgcccgcatc cggcacgcgc atccgtccgc gatcgacgat ctcgcggcgg
      421 acttcccgga cctgaagatc atcatggcgc atcccggctg gccgtgggtg gacgaggcga
      481 ctgcggtggc gctgcacaag ggcaacgtct attgggagat gtcgggctgg gcgcccaagc
      541 atttcccggc ccagctcaag gtggacatgc gcgcgcggct ccaggacaag gtcctgttcg
      601 gcagcgatta ccccagcatg ccgtacgcgc gcatcctcgg ggagtggcag gaactcggct
      661 acggcgatgc ggtgatggag aagatctttc accgcaacgc cgagcggatc ctgggcctct
      721 agcagcctgt cggactatgt ccgatgagcg gtcggtgtga acacatagga agtcgatcct
      781 gggtaacgcc gagccgacag gctgtaatgg ggcatttatt ggccgggaac cgttgttcag
      841 gtgccctgca tgcgctctac gttgtaggca aggcacacca aattccattc ccctctgacg
      901 cgattcacgc cccgcgcgag gaagtgctgg agcttcatca ccggcctgat aatcccaatg
      961 ttccatttag cagtgtgtcg tccgcggaca ccggagatca tgatgcgctc ggcgcacgaa
     1021 acccggtgcg gaaaccgaag tccgacacac tgctagtcgg cggtgatctt cgccgcggcg
     1081 atgaacttct tccagcgcgc cacgtcggcc ctgatttgcg ccgcgatggc ctccggagag
     1141 ctggtttgcg ggacgaggcc ctgcttgagc agcgattcgc ggacgtcgga cagcgcgagc
     1201 gccacggcga gctcgcggtt gaccttgagc acgacatccc gcggaatgcc ggtcggcccc
     1261 gcgaaccaga agtagaggtc gatgtcgagg ttcttgagcc ccgactcggc gaaactcggc
     1321 gaatccggcg ccagcaccga gcggctctcg cccgataccg cgatcacgtt gagccggccg
     1381 gccttcgcgt gcggcagcgc ggtgtggacc ggcagcacgg caagctggac ctggccgccg
     1441 aggagatcgg cgagcgcgcc cgccgcaccc ttgtacggca cgtgcagcac gtcgagtccg
     1501 agccgctgct tgagcaactc cacggcgagg tgctgcggcg tgccgttgcc gggagaggag
     1561 tagctcagct tgcccggccg cgcgcgcgcc gcggcggtga gttcgtcgag ctttttcacc
     1621 ggcagcgccg tggagttgac cacgagcgcc aggctgccgg tggcggcctt gccgatcggg
     1681 gcgaagtccc tgagcgggtc gtagggcagg tccttgtaga gcgccggggt gatggtgaac
     1741 gtgttgacgg tcaccatcag cgtatggccg tccggctggg atttggcgac gatgtcggcg
     1801 ccgaggttgc cgctcgcgcc cggccggttg tccacgatca cgggctggtt ccagcgctcg
     1861 ttgagcttgg gcccgacgag gcgggcgatg atgtcgatgc ccgttccggg cgtatagggc
     1921 acgaccagcc ggatcggccg ggcgggatag gcctggccga gggcgagcgc cggacaagcc
     1981 agcgcgaggc acagcagcag catccgcaat gcagagcgag ccatttgcct ttcctccctc
     2041 agggtgcggg gccggcctgc ttgcccgacg caaccgccat tcgcagcagc cgcatcgcat
     2101 tctcgaaata gatcttgcgc ttgtcggatt cgggcagctt gaggtcctcg accgagcgca
     2161 tcccctcgcg aatgaacatc ggcccgccct ccggatcgaa cgggcaatcg ctcgcgaaca
     2221 cgacccggtc cgcgccgaag aaatcgagcc cgcagcgcag cgcggaggcc gacccgccga
     2281 gaacggtgtc cgcgtagaac atcctgaaat actcgatcgg cttcttcgcc atgcgcctga
     2341 gcagttcctc gtagttctcg tccactgagc ggctgccgag ttgcgcccaa agcgtctcgc
     2401 agcgtcccgc gaagaacggg atcatcgccc cgcagtggtg ggtgacgatc cgcatgtccg
     2461 gcagcgcctc gaacaggccg gaaaacacga tgcgcgccat cgccacgctg gtctcgtacg
     2521 gccagccgag cacctgccag atctcgtact tcgaccctgt ctcgttcacg tagtcggggc
     2581 gcgacgcggg gcgcgccggg tgcatccaga tcggcagatc gtgcttcctc gtggcgcgct
     2641 cgaacaccgg gaggaactcc ggatcatcga gcggccggcc gttcacgctg gtgagaatct
     2701 gcaccccgcg cgcgccgagg cgggccacgg cgcggtccat ctcctccagc gccgccggca
     2761 cgttgttcat cggcagcgag gccacgaacg cgggaaattt cttcggccac cgcgcgcaca
     2821 tctccgccat gccgtcgttc gcgatgcgcg caagctcggg cgagagctcg ggcccgccca
     2881 ggagctccgg ggcgggcagc gaaagcgtca gcacctgctg gagatccggc cactgatcga
     2941 gcatcgtgac gcgcgactcg atgtcccaca gcatgcgcag gctcgacatg cgcttcacca
     3001 ttccgggctc cttcgagtgc tgcttcacct tctcgaggta gcgcgcgggc atcacgtggt
     3061 tgtagaggtc gatcttcatg ttcgcggcga acgcggcatc gcgcgccggg gcgcgcccgg
     3121 cacaggccac tgcgcgttca tattaacatc gttccccgcg tcggcgcagg cggccatcgg
     3181 caacgacaaa cagcgcgcgc gtcgcgtgga atcggtaccg atgcggtcat gcgcgcgccg
     3241 ttttaatgtt aggatttcgt ttgtcgccca tgtaaagttc aaggagaagc gatgagtcgc
     3301 aaggtcgtcc tggtcgtgcc ggaagcgctg gtgcccgagt ccgcagtcac cgccgagagc
     3361 cgcaaggccg gcggcagcgg gttgcggctc ggcgtgctcg acaacagcaa gggcaacgcc
     3421 gaccacctcc tcaagttcat cgtggagggc gtgaaggcgg cggtgccggt tgcgtcggtc
     3481 gtgtcgctga ggaaggcgag cgtcagtctg ccggcgcctg gagccatcct cgaccagctc
     3541 accgtggaag ctgatttcgt cgtcagcgcg atggccgact gaggctcatg cacgtcgtgg
     3601 agtgtccacg acatgtgtga gctccggaag cgcggcctcg tcaccgccgt gatctgctcc
     3661 gagcccttcc tcaagctggg gaagacgcag gcgcgcgtgt tcggcgtgcc ggacttgccg
     3721 ctcttgctga tcccgcaccc gctgggcggc ctctcgctgg agcaggtgga ggggcgggcc
     3781 gcgcacgcga tcccaaaagt gctcaagctc atcgggaaac atttcaaatg acggacctcg
     3841 ccgattttct gagggcgcct ggcgcgagcc tcgaatgcga ggacgatttc gacaccgtga
     3901 acgagctcgt gcgggcccgc ggctggagcg acgggctgcc ggtcgtgccg ccaaccgctc
     3961 agcgcgtcga gcgcatgctc gcctactgcg accggccgtg gggcgagccg gtggcgaagg
     4021 tggcgccgcg ctacggggag gcgacgccgc tgcggctcgc cgccaacgcg gtgatggcag
     4081 gctgccgccc ggaatatttt ccgctgctcg tgctcgcgat cgaggcgatg tgcgaggagc
     4141 cgttcaacct ctacggcgtc caggcgacga cgcatctgtg cgcgccgctc gtgatcgtca
     4201 atgggccggt ggcgaaggag ctcgacatca attccggcca caacgcgttc gggccgggct
     4261 ggcgggcgaa cgccaccatc gggcgcgcga tccggctcgc actggtcaac atcggcggcg
     4321 cgatccctgc gctcggcgac atgtccacct tcggcgcacc gtccaagttc agctacctcg
     4381 ccgccgagaa cgaggaggcg agcccgtggg agccgctgca cgtcgaacgg gggctcccac
     4441 gcgaggcaag cgcggtgacg gtgatcggtg cggaatgccc gcacaacgtg aacgaccacg
     4501 agagcatcag cgccgaaggc atcctcacca ccatcgccgg caccatgctc ggcaccggcg
     4561 cgaacgacgt ctactacgag gcgcagccgc tcatcgtcat gggaccggag cacgcgaaga
     4621 ccgtggcggg cggcggatac agcaaggccg acgccaagcg tttcctccac gagcatgcgc
     4681 acctgccgct gggccgcttt tcgagggaga acatcgaacg caggctccgc gtcacgtgga
     4741 aggagcgcta tgctaacgcc ggtccggacg cgccggtgta catggtgcag cgccccgagg
     4801 acatcctgat cgcggtgatt ggcggcgcgg gcaagcactc ggccgtgatc cagaccttcg
     4861 gcgctaccaa ggccgtcacc cgcgccctca agacgcgcga cggaagctac gcccgctcca
     4921 tcgcggactt tcggaagaag tgataagtga caagtgatgg gtgataggag ttgaatacgg
     4981 ccagtggccg ttcgcccaaa ccgcttttca ctcagtccct atcacttatc acctatcact
     5041 catcacgcct catcaagaat atgaagtgct actacatcaa atccgtcaac ggcagaaccg
     5101 tgttcgagcc gcgagaagcg cccaagccgc aacccaaggc gggagagatg ctcgtgcgcg
     5161 tgcgcgcggc ctcgctcaac cgcggcgaga tcctcgccag catcagcctg caccaggtgc
     5221 acgagccgca tcccgcgggc ggggactgcg cgggcgaggt ggaagcggtg ggcgaaggcg
     5281 tcaccgcgtt caagcccggc gatgcgatcc tggggcgcgc gcgcggctcg ttcgccgaat
     5341 acgtcgtgat gggcgccgcg caggcggcgg cgaagccgca acggctcacg tgggaacagg
     5401 cggcggcggt gccgatcgtg tcagtcacgg cttacgaggc catctgccag tacggcaaac
     5461 tcaagtcggg cgaaacgctg ctcgtcgccg gcgcggcctc cggcgtgggc gtgatcgccg
     5521 tgcaggcggg caaatatctc ggcgcgaaag tgatcggcat ctcgcgctcg aacgataagc
     5581 ttgagaagct caaggtcatc ggcctcgacg tcggcatcca ggcggcggga ggcggcttcg
     5641 cggacaaggt gctggaggcg accggcggca agggcgtcaa cctggcctta aatctgatcg
     5701 gcggcagcgc gtttcccgac ctcgtgcgct cgctcgccaa ccaagggcgg cttgcgatca
     5761 tcggctacgt ggacggccag caccatgccg agatcgacct cgagacggtg cacggcaagc
     5821 gcttgcagat cttcggcgtt tcgaacgcgc tgatgaccgc gccgcaacgg gcggaagcga
     5881 tgcgcggatt tgcccgcgac ctgctgcccg gcttcgcgga agggcgcatc acgcccgtgg
     5941 tggacaaggt gtttcccttc aacgagctgc cggcagccaa ggcctacgtc gagaccaacg
     6001 cgcaactcgg caaggtcgtc gtcacggtcc cgtgacgacc aaaggatgaa ggatcaagga
     6061 tgaaggatga cctgctcttt actcccgcgc tgcgggggtc atccttccac gttcagcctt
     6121 catccttcag gcgaagccgt ctgtaggtca tccttccgcc ttctgccttc atccttcgtc
     6181 gattgacgcg cggccgcgcg tcaatcgatt ttcgctccgg atcgc
//
LOCUS       DBNGWZ010000377         3659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1430898, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000377 DBNGWZ010000000
VERSION     DBNGWZ010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1430898"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC16CX_11065"
     CDS             complement(<1..629)
                     /locus_tag="AC16CX_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7125 family protein"
                     /protein_id="HIR3013085.1"
                     /translation="MAKAKSIYSCTECGGQVLKWQGQCPHCQAWNTLVETVAEAAHTA
                     NRFGLAAEAGKLQRLSEIEAREESRVPTGIEEFDRVLGGGLVPGGVVLLGGDSGIGKS
                     TLLLQSLAGIGGARKVLYVTGEESTEQVALRARRLALDARGVHVLPEINLEKIQATLT
                     AERPEIAVIDSIQTLYSGQLQSAPGSVAQVRECAAQLTRVAKSGGTAIVL"
     gene            complement(683..1648)
                     /locus_tag="AC16CX_11070"
     CDS             complement(683..1648)
                     /locus_tag="AC16CX_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013086.1"
                     /translation="MRFVLRAAVSIVVLCVAPVAWGQSFPTKPIRMIMPWNPGGTTDT
                     TARILGQKMTERWGQPVVIDGRPGASGIIGTEIAMRAPADGYTLLHANMSQWATAPSL
                     YKTNYDVLRDFAPISLVATAPQLLVVPLSLRATTVQELIQLAKAEPGKLNFGSGGAAT
                     LAYLGGELFKKLAGVNVVHVPYKGTIFAVTDVMGGRMQIVFSDMPIALPHARSGKLRA
                     LAVTGAKRTPLVPDMPTVAETGLPGYVIENSWGVVAPKGVPAPIIAKLNAEIVRVHNL
                     QDVKDRYAVLGLDAVSSTPEKFTEIIRADTDRFSKLIKETGAKAD"
     gene            complement(1788..2195)
                     /locus_tag="AC16CX_11075"
     CDS             complement(1788..2195)
                     /locus_tag="AC16CX_11075"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR3013087.1"
                     /translation="MALSLGIRQRPSTFHRYAVAVGVVALAFALRYGIYGTLDHRLPF
                     AFFLPATIIAAWYGGLGPGLLATLAGLLLGDYFFLPKQEAGGSVGEPERTVIGLYALN
                     SALIVLLFWHLHTRLRDVDDELRKLKGGEDQAK"
     gene            complement(2256..3323)
                     /gene="alr"
                     /locus_tag="AC16CX_11080"
     CDS             complement(2256..3323)
                     /gene="alr"
                     /locus_tag="AC16CX_11080"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3013088.1"
                     /translation="MMRPLFATIHLDALRQNLAVVRKHAPHSTVLAVIKANAYGHGLL
                     RAAAALSEAEGYALLELEEAVRLREAGFRQRIVLLEGFFEPGELRVLLQHRLTAVVHS
                     FDQVAVLDGLPVDAKLEVLLKVNTGMNRLGFSPADYPAALERLRRNPGIGAITLMTHF
                     ANADDARGVEWQMERLRQLAGHDTLPLSLANSAAILRYPETHADSVRPGIMLYGCSPF
                     PGKTGDEAGLRPAMTLESSLIAVQQLKPGDTVGYGDLFRAERAMRIGVVACGYADGYP
                     RHAPNGAPVRVAGRMTGTVGRVSMDLLCVDLTEVPQARVGSRVVLWGEGMPVERVAQA
                     AGTVGYQLLCALAPRVRIVEQ"
     gene            complement(3391..>3659)
                     /locus_tag="AC16CX_11085"
     CDS             complement(3391..>3659)
                     /locus_tag="AC16CX_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013089.1"
                     /translation="AADEATTASEPLAIPRPPGETVTMAIRRLVRTYPMLNRRKLMAE
                     ASQFMAQHALQGRPAAEVIDELEVVFTRHYEQHKSKGERRKSKG"
BASE COUNT          629 a         1240 c         1141 g          649 t
ORIGIN      
        1 agaacgatcg cggtgccccc gcttttcgcg acgcgggtga gctgcgccgc gcactcgcgc
       61 acctgcgcga ccgagccggg cgccgactgc aactgtccgg agtaaagcgt ctggatcgag
      121 tcgataaccg cgatttccgg tctttccgcc gtcagcgtcg cctggatctt ctcgaggttg
      181 atctcgggca gtacgtgcac gccgcgcgca tcgagggcga gccggcgcgc gcgcagcgcg
      241 acctgctcgg tggattcctc tccggtcaca tacaggacct tgcgcgcgcc tccgatcccg
      301 gcaagcgatt gcagtagcaa agtggacttg ccgatgccgg aatcgccgcc gaggagcacc
      361 actccgcccg gcacgagccc gccgcccagc acccggtcga actcctcgat gccggtcggg
      421 acgcgggatt cctcgcgcgc ctcgatctcg gaaagacgct gcagcttccc cgcttccgcg
      481 gccagaccga agcggttggc ggtgtgcgcg gcttccgcga cggtctcgac cagcgtgttc
      541 caggcctggc agtgcggaca ttggccctgc cacttcagca cctggccacc gcactccgtg
      601 caggaataaa tggatttggc ctttgccatt cgtggcttgt ggctggtggc tcgctgctga
      661 cggctgacag ctgacggctc gttcagtcgg ccttcgctcc ggtttctttg atgagcttcg
      721 agaacctgtc cgtgtcggcg cggatgattt cggtgaactt ttcaggcgtg ctgctgacgg
      781 cgtccagccc caataccgcg tagcggtcct tgacgtcctg caggttgtgc acgcgaacga
      841 tttccgcgtt gagcttcgcg atgatgggcg cgggcactcc tttcggcgcg acgacgcccc
      901 acgagttctc gatcacgtat cccggcaggc cggtctccgc cacggtcggc atatcgggta
      961 cgagcggcgt gcgcttcgcg ccggtgaccg caagcgcgcg gagcttgccg gatcgggcgt
     1021 gaggcagcgc gatcggcatg tcggaaaaaa cgatctgcat ccggccgccc atcacgtcgg
     1081 tgaccgcgaa gatcgtcccc ttgtacggga catgcaccac gttcaccccg gcgagcttct
     1141 tgaacaattc gcctccgaga tacgcaagcg tcgccgctcc gcccgaaccg aaattgagct
     1201 tccccggctc cgcctttgcg agctggatca gctcctgtac ggttgtcgcc cgcaagctca
     1261 ggggaaccac cagcagctgc ggcgcggtgg cgaccaggct gatcggcgca aaatcgcgca
     1321 aaacgtcgta gttggtcttg taaagactcg gggccgttgc ccactggctc atgttggcgt
     1381 gcaacagcgt gtagccgtcg gcgggggccc gcatcgcgat ctcggtaccg atgatcccgc
     1441 tcgccccggg acgcccgtcg atcacgaccg gctggcccca cctctcggtc atcttctgac
     1501 ccagaatccg cgccgtcgtg tccgtcgtcc ctcccgggtt ccacggcatg atcatccgta
     1561 tcggcttggt gggaaagctc tgcccccacg ccacgggcgc aacacacagc accacgatgg
     1621 agaccgctgc acgcaagaca aatctcattc gggtccccct cctgttcgtg attcgtggct
     1681 cgtggctgac ggctagatgc agggtatcaa cgcccgctcg ctctgtcacg cgtacacaac
     1741 cgctcccccc gctgtgcgcg atccccgggg tagccctagc gtgccgctta tttggcctgg
     1801 tcttcgccgc ccttcagctt ccgcagctcg tcgtcgacgt cgcgcaggcg cgtatgcaga
     1861 tgccagaaca acaacacgat caacgcgctg ttcagggcgt agaggccgat gacagtgcgc
     1921 tcaggctccc ccaccgaccc gcccgcttcc tgcttcggca gaaagaaata atcgcccagc
     1981 agcaggccgg ccagtgtcgc aagtaacccc ggtccaaggc cgccgtacca cgcggcgatg
     2041 atggtggcgg gaaggaaaaa ggcaaacggc aggcggtggt cgagcgtgcc gtagatgccg
     2101 tagcgcagcg caaatgcaag agccaccacg ccgacagcaa cagcgtatct gtggaaggtg
     2161 gagggccttt gccgtatgcc gagggaaaga gccatggctg aacgataata caccccgcga
     2221 tacccgcttt caccgtgcca ccaaaccgcg ggcgctcact gttccacaat acgaacccgc
     2281 ggcgcaagcg cgcacagcag ttgatagccc acggtgcccg ccgcttgcgc gacccgttcc
     2341 acgggcatgc cttcgcccca cagcacgacg cgcgatccga cgcgcgcctg cgggacctcc
     2401 gtgaggtcca cgcacagcaa gtccatggag acgcgcccga cggtgcccgt catccggccc
     2461 gccaccctga ccggcgctcc gttcggcgca tggcgcgggt agccatcggc gtagccgcag
     2521 gcgacgactc cgatccgcat cgcccgctcc gcccggaaca ggtcgccata accgacggtg
     2581 tcgcccggct tcaattgctg aaccgcgatg aggctgcttt cgagcgtcat ggcgggccgc
     2641 aaccccgctt catcaccggt tttcccggga aacggcgaac agccgtagag catgatgccg
     2701 ggtcgcaccg aatctgcatg ggtttcggga taacgcagga tcgctgcgga gttcgcaagg
     2761 ctgagcggga gcgtatcgtg tccggccagc tgccgcaggc gttccatttg ccattccacc
     2821 ccgcgcgcgt cgtcggcgtt cgcgaaatgc gtcatgagcg tgatggcgcc gataccgggg
     2881 ttgcgccgca gccgctcgag cgctgctgga taatccgctg gagagaatcc cagccggttc
     2941 atgccggtat tcactttgag cagcacttcg agtttcgcgt ccacaggcag cccatcgagc
     3001 actgccacct ggtcgaagct gtgaaccacg gcggtcagcc ggtgctgcaa cagcacccgc
     3061 agctcgcctg gttcgaaaaa tccttcgagc agcacgatgc gctgcctgaa cccggcttcg
     3121 cgcaaccgca ccgcctcctc cagctcgagc agggcatagc cctccgcctc cgaaagcgcg
     3181 gcagccgccc tcagcagacc atggccgtag gcattggcct tgatgacggc caacaccgtc
     3241 gagtgcggcg cgtgcttgcg caccaccgcg aggttttgcc tcagtgcgtc aagatggatg
     3301 gtggcgaaaa gggggcgcat cagccgaagg cgaaaggcaa aaggcgaaag gcgaaagtga
     3361 aaccgttttt gtggaagtcc aacaagggca ctaccctttc gactttcgcc tttcgccttt
     3421 cgacttgtgc tgctcgtagt gtcgcgtgaa aacgacctcg agctcgtcga ttacctcagc
     3481 ggcgggccgc ccctggagcg cgtgctgcgc catgaattgc gaggcttccg ccatgagctt
     3541 gcgccggttg agcatgggat aggtgcgtac gaggcgtcgt atcgccatcg tgaccgtttc
     3601 gccgggtggg cgcgggatgg cgagcggttc cgacgcggtg gttgcctcgt ccgccgcgc
//
LOCUS       DBNGWZ010000378         6428 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_672356, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000378 DBNGWZ010000000
VERSION     DBNGWZ010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_672356"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="AC16CX_11090"
     CDS             complement(<1..969)
                     /locus_tag="AC16CX_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3013090.1"
                     /translation="METRRNVMVVTAALLALLILTPLTGSYVIVLVTHALIFAILAMS
                     VDILLGYTGLPSLGQAAYLGMGAYLTAILAARFQFGLDWTFWLVIVMGILIGAATAAL
                     FGLLALRAAGVYFLMITLALAMCVWGLAYRWNSLTGGDNGIVLPPRPAFGIELADDVT
                     YFYLVFGFFAVSLAMLYVLVRSPFGRSLVGIRENELRMRILGYNTWLHKYIAFIIAGG
                     FGGLAGVLWAHAHGLVSPEDVTLATSVDALLMVVLGGSGTLVGGAIGAGIVVFLREYL
                     STLVPWWQYVLGGVYVLTILYLPGGLMSIPERVRSWRAQSPGAGEAK"
     gene            complement(980..1840)
                     /locus_tag="AC16CX_11095"
     CDS             complement(980..1840)
                     /locus_tag="AC16CX_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3013091.1"
                     /translation="MDAQFWLIQAFNGISYGALLFLLASGLSLIFGVMRIVNLAHGSF
                     FLLGGYVALTVIWTTGSWLLALPVAALVIAVVGLLMERVFLRPLGFDPLRQVLLTVGF
                     AYLFQQAALDIWGGDNLDITPPHVLTESIVLGGLHLPAYRIFMICMAVVMGLILWLTM
                     EKTRIGAMIRATVDDSEMARGVGIDTSRVSMFIFALGAFLAALGGVIGGAFLGVYPGL
                     DFAILPIAFAVVIIGGMGSLTGAAVGSLLVGLADNFGKALFPEVSYFTLYAPMVLILA
                     LKPTGLFGRD"
     gene            complement(1874..2935)
                     /locus_tag="AC16CX_11100"
     CDS             complement(1874..2935)
                     /locus_tag="AC16CX_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3013092.1"
                     /translation="MTGEMPLTQTSAVDTRDALAGLIPVSIVTGFLGSGKTTLINRLI
                     KRPEMNRVAVIVNELGEIGVDNELVQVSSEQMMLLNNGCLCCVLRGDLQETLRELFVK
                     RRNGEVIDYDRVVIETTGLADPAPVMQTLITDTMLLEQYRLDCVVTLIDAVNSPGQLK
                     QFSEPVKQVALADRLVVTKSDLVGEKQLAALTERLHEINPNAPVRIALNGEIELSFLV
                     DVGLRRTRAQLDEVERWLGAEEEDEHGHRHDETVQSFVLRLEKPMQWATFTQCMEVLT
                     ALRGPDLLRVKGLVNVEGRSGPMVVQGVQHLFHPPLELAAWPGTDRSTRLVFITRGIP
                     RQTVADLFAAIGAIGSVTN"
     gene            complement(2949..4196)
                     /locus_tag="AC16CX_11105"
     CDS             complement(2949..4196)
                     /locus_tag="AC16CX_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3013093.1"
                     /translation="MAAEGALRPGGQATPLALNMKLLAHHELAGFGGIGEGVNMQKTK
                     DGRRILWLAHESAPKNFTAVDVTDPRNPKMVVQTDLPHADVRSNSLDVVGDIMAVAYQ
                     TRKWGLKPAGFDLFDISKPEQPRLISHFDTSGPCSRGAHCVWFVDGEYVHLTSGAPDF
                     EPTHEKDDQFYRIIDVRNPSKPTEVGRWWYPGTRKGDSDAPPPRHPKFDGGYRPHNTN
                     VYPRRPDRAYIGYIDGGAWILDISDKSRPRPVGHLQYSPPYNGFTHTVLPLLERGFLI
                     ISDECVRDNGEDWPKLAWVVDARVESNLVPVATLPLPPAEVFIRRGGRFGAHNLHENL
                     PGPCSFRSETLIFGTFFNGGVRAYDVSNPYQPKEVGYFVPGTPRLAPKGSAQVNDVWV
                     DENRLVYAVDRFTGGLYTLETTF"
     gene            complement(4424..5356)
                     /locus_tag="AC16CX_11110"
     CDS             complement(4424..5356)
                     /locus_tag="AC16CX_11110"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013094.1"
                     /translation="MRNRRSSTARKRGSKGVVGQIGLGIMGGAFAKHLLAAGFNVIGY
                     DPAAAAARALARAGGRPAGSCAEVAARTRIVITSLPSPAAMEEAFFGKQGIATGARPG
                     SIVIEASTMTLELKESIRRRLARRGVVLLDCPISGTGAQAAAKDIAVYASGDRRAFNR
                     CRRVFDGFARSTYYCGEFGIGSKLKFVANLLVTIHNLSTAEAMVVGEKAGIHLGLLYR
                     VIADGAGSSRMFQVRGPMMVRGRYLPPHMKSKIYQKDIDIIRAFVRRLRCPTPLFDGS
                     IPYYTAALRQGWGMHDTAAIHSVLRKRAGLRGRP"
     gene            complement(5367..5507)
                     /locus_tag="AC16CX_11115"
     CDS             complement(5367..5507)
                     /locus_tag="AC16CX_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013095.1"
                     /translation="MTAPPDSTGAVIPLELAPAGRKPEAGIQETLNLESGCHRNDDSL
                     MR"
     gene            complement(5504..>6428)
                     /locus_tag="AC16CX_11120"
     CDS             complement(5504..>6428)
                     /locus_tag="AC16CX_11120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013096.1"
                     /translation="AALAQGWKPERNVELIVPASAGGSLDLTGRTVHKIWDDLKLLPV
                     SSTVANRSGGGHAMAYNFLNQRAGDPHYLSITSSTILTNQINGRIPMTYSDFTPLAVL
                     LTEHIAFVVRSDSPLKTGKDMIEALRKNPASLSIALGSAIGGTHHIAVGLPLQSAGVD
                     IKQVKLVGFNSTAEAFTALLGGHVDVANGGTANAAILVESGKLRVLTVTSSKRMQGPL
                     AAAATWPELGYKGVFDNWRGVIGTKGMTAAQVAYWENVFKRIASSEEFLQYAAKNQWE
                     NSYRGPAEALRFMEAQYGELKSVMTFLGLAK"
BASE COUNT         1176 a         2158 c         1984 g         1110 t
ORIGIN      
        1 ctttgcctcc cccgcccccg gtgattgtgc acgccagctg cgaacccgct ccggtatgct
       61 catcaggccg cccgggagat agagtattgt aagcacgtac accccgccca atacgtactg
      121 ccaccacgga acgagcgtac tcaggtactc ccgcagaaag acgacgattc cggcaccgat
      181 ggccccccca accagagtcc ccgagccccc cagcaccacc atcaagagcg cgtccaccga
      241 agtggcgagc gttacgtctt cgggactgac gaggccgtgc gcgtgcgccc acagcactcc
      301 cgcgagcccc ccgaagccgc cggcgatgat gaacgcgatg tacttgtgca gccacgtgtt
      361 gtatcccagg atgcgcatcc gcagctcgtt ctcgcggatt cccaccaggc tgcgcccgaa
      421 cggggatcgg accagtacat acagcatcgc cagcgaaacc gcgaagaaac caaacaccag
      481 atagaaatag gtcacatcgt ctgcgagctc gatgccgaac gccggccgcg gcggcaggac
      541 gatcccgttg tccccgccgg tcagggaatt ccaccggtag gcgagccccc atacgcacat
      601 cgccagtgcc agtgtgatca tcaggaaata gacgccggct gcgcggaggg cgagcaggcc
      661 gaacagcgcc gccgtcgccg ccccgatcag tattcccatc acgatcacca gccagaacgt
      721 ccagtccaga ccgaactgga accgcgcggc cagaatcgcg gtcaggtagg cgcccatccc
      781 caggtacgcg gcctggccca gcgaaggcaa ccccgtgtat cccagcagga tatcgacgct
      841 catcgccagg atggcgaaaa tcagcgcgtg cgtgacgaga acgatcacgt acgaaccggt
      901 gagaggcgtg aggatcagga gcgccagcaa ggccgcggtg accaccatga cgttgcggcg
      961 ggtttccatg gatacagcct caatcccgtc caaaaagacc ggtcggtttc agcgccagga
     1021 tcaggaccat tggcgcgtag agtgtgaaat aggaaacctc cgggaacagc gctttcccga
     1081 agttatcggc gagcccgacg agaaggctcc ccacggcggc cccggtcagg cttcccatgc
     1141 cgccgatgat cacgacggcg aaagcgatcg gaagaatcgc gaagtccagt cccggataca
     1201 cgccaaggaa ggcgccccct atgacgccgc ccagcgcggc caggaaggcg cccaatgcga
     1261 agatgaacat ggaaacgcgg gaagtgtcta tgccaacgcc gcgggccatt tccgaatcat
     1321 cgacggttgc gcggatcatg gccccgatgc gcgtcttctc catcgtgagc cacaggatca
     1381 aacccatcac gaccgccatg cagatcatga agatccgata cgccggcaaa tgaagtccgc
     1441 cgaggacgat gctctcggtc agtacgtggg gcggcgtgat gtccagattg tcgccgcccc
     1501 agatgtcgag agccgcctgc tggaacagat acgcgaaacc taccgtcagc aatacttgcc
     1561 ggaggggatc gaaccccaag ggcctcagaa agacccgctc catgagcagg cccaccacgg
     1621 cgatgaccag cgcagccacg gggagcgcca gcaaccacga gcccgtggtc cagatcaccg
     1681 tcagcgcgac atagcctccc agcaggaaaa aggagccatg cgcaagattc acgatgcgca
     1741 tgacgccgaa aatcagggac aggccactcg ccagcaggaa cagcagggcg ccgtaggaga
     1801 tcccgttgaa ggcctgtatc agccagaatt gcgcatccaa tcccccctcc tccgcctcgg
     1861 accggctgac agtctagttc gttacagatc cgattgcacc gatcgccgcg aacaggtcgg
     1921 cgaccgtctg gcgcggtatg ccgcgcgtga tgaacacaag ccgcgtgctg cggtcggttc
     1981 ccggccacgc cgccagctcg agcggcgggt ggaacaggtg ctgtacgccc tgcaccacca
     2041 tcggcccgct cctgccttcc acgttgacca gcccctttac gcgcagcagg tccgggccgc
     2101 gcagcgcggt caggacctcc atgcactgcg tgaacgtggc ccactgcatc ggcttttcga
     2161 ggcgcagcac gaacgattgc acggtctcgt cgtgccggtg gccgtgctca tcttcctcct
     2221 ccgcacccag ccagcgctcg acttcgtcga gctgcgccct cgtgcgccgc aacccgacgt
     2281 ccaccaggaa cgaaagctcg atctcgccgt tcagcgcgat gcgcaccggc gcgttcggat
     2341 tgatctcgtg gagccgttcc gtgagcgccg caagctgttt ttcgcccacg aggtcggatt
     2401 tggtcaccac caaccggtcc gcgagcgcga cctgcttcac cggctccgag aactgcttga
     2461 gctggccggg gctgttcacc gcgtctatca gcgtcaccac gcagtcgagg cggtactgtt
     2521 cgagcagcat cgtgtcggta atcaacgtct gcatcaccgg cgccgggtca gcgagcccgg
     2581 tggtctcgat caccacgcgg tcgtagtcga tcacctcgcc gttgcggcgc ttgacgaaga
     2641 gttcacgcag cgtctcttgc aggtcgccgc gcagcacgca gcacaagcac ccgttgttga
     2701 gcagcatcat ctgctcggaa gacacctgta cgagctcgtt gtccacgccg atctcgccca
     2761 gttcgttcac gatcaccgcg actcggttca tctcgggccg cttgatgagc cggttgatga
     2821 gcgtggtctt gccgctgccg aggaagccgg tgacgatgga caccgggatc agacccgcga
     2881 gcgcgtcgcg ggtatcaact gcacttgttt gcgtcagcgg catttcacca gtcacgagtc
     2941 acgcctcatc agaatgtagt ctccagcgtg tacaggccgc cggtaaagcg gtccaccgcg
     3001 tagaccagcc ggttctcgtc cacccagacg tcgttgacct gcgccgagcc cttgggcgcg
     3061 agcctgggcg tgccgggcac gaagtagccg acttccttcg gctggtacgg gttggagacg
     3121 tcgtaggcgc gcacgccgcc attgaagaag gtgccgaaga tcagcgtctc ggaacgaaag
     3181 gaacacgggc cgggcaggtt ctcgtgcaga ttgtgcgcgc cgaagcggcc gccgcgccgg
     3241 ataaacacct ccgccggcgg cagcggcagc gtggccaccg gcaccaggtt gctttcgacc
     3301 cgcgcgtcca ccacccaggc gagcttgggc cagtcctccc cattgtccct gacgcactcg
     3361 tccgagatga tcaggaaccc gcgctcgagg agcggcagca cggtgtgcgt gaagccgttg
     3421 tagggcggcg aatactgcag atgcccgacc ggcctggggc gcgacttgtc gctgatgtcc
     3481 agtatccagg ccccgccgtc tatgtaaccg atgtaggcgc ggtcggggcg ccggggatag
     3541 acgttggtat tgtgcggccg atagccgccg tcgaacttcg gatggcgcgg cggcggcgca
     3601 tccgaatcgc ccttgcgcgt gccgggatac caccagcgtc cgacctcggt cggcttcgac
     3661 gggttgcgca cgtcgatgat gcggtagaac tggtcgtcct tctcgtgggt cggctcgaaa
     3721 tccggggcgc cggaggtgag gtgcacgtat tctccatcca cgaaccacac gcaatgcgcg
     3781 ccgcgcgagc acgggccgga ggtgtcgaag tgcgaaatca gcctgggctg ctccggtttg
     3841 ctgatgtcga acagatcgaa gcccgccggc ttcagccccc acttcctggt ctgataggcg
     3901 accgccatga tgtcgcccac cacgtcgagc gagttcgacc gcacgtcggc atgcggcagg
     3961 tcggtctgca ccaccatctt cgggttgcgc gggtcggtca cgtccaccgc cgtgaaattc
     4021 ttgggcgcgg actcgtgcgc gagccacagg atgcgccggc cgtccttcgt cttctgcatg
     4081 ttgacgccct cgccgatgcc gccgaagccg gcaagctcgt gatgggcgag cagtttcatg
     4141 ttgagggcga gcggtgtcgc ctgcccgccc ggtcgtaaag cgccttcggc tgccatttac
     4201 cttcctttcc tggttatgcg ccaaggagtt cgccagcacg aaagccggcg aactcctgag
     4261 gcaaaaccgc ctgccagaaa acggcaggac ggactaaaca agctgacccc accctcccat
     4321 ttccacgatg gcagcgaaat attcttttct atccagcctc attgtaggtc ttcaggcggt
     4381 tttgcgttta gcagcttgtt tcgccgccgg gcgcgacaag ctgctaggga cgtcccctca
     4441 agccggcgcg ttttctgaga accgagtgta tcgcggccgt atcgtgcatg ccccacccct
     4501 gcctgagcgc cgcggtgtaa tacggaatgc tgccgtcgaa cagcggcgtc gggcatctca
     4561 gccgccgcac gaacgcccgg atgatgtcga tgtccttctg gtagatcttc gacttcatgt
     4621 gcggcggcag atagcgcccg cgcaccatca tcgggccgcg cacctggaac atgcgcgagc
     4681 tgcccgcgcc gtcggcgatg acccggtaca gcagcccgag gtggatgccc gccttttccc
     4741 ccactaccat cgcctcggcg gtcgagagat tgtgtatcgt caccagcagg ttggcgacga
     4801 acttgagctt ggagccgatg ccgaattccc cgcagtaata ggtcgagcgc gcgaagccgt
     4861 cgaacaccct gcggcagcga ttgaatgcgc ggcggtcccc gctcgcatac accgcgatgt
     4921 ccttcgcggc cgcctgggca ccggtaccgc tgatcgggca gtcgagcaga accactccgc
     4981 gccgtgccag gcgccggcgg atgctctcct tgagctccag cgtcatcgtg ctcgcttcga
     5041 tcacgatcga gccgggacgg gcgcccgttg cgatgccctg ttttccgaaa aacgcttctt
     5101 ccatcgccgc gggcgacggc agcgacgtga tgacgatccg cgttcgcgcc gcgacttcgg
     5161 cgcatgagcc cgccgggcgt ccgccggccc gggccaaagc cctggctgcg gcggctgcgg
     5221 ggtcgtagcc gatgacattg aaacccgccg ccaacaggtg cttcgcgaaa gccccgccca
     5281 tgatgccgag gccgatctgc cctacgacgc ctttgctgcc cctcttccgg gctgtgctcg
     5341 atctgcggtt tcgcatcccc gaattcctag cgcatcaacg aatcgtcatt ccggtggcag
     5401 ccggattcca gattgagcgt ttcctggatc ccggcctccg gcttacggcc tgccggggca
     5461 agttccagcg ggatgaccgc gcccgtgcta tcgggtggcg cggtcatttc gcaagcccca
     5521 ggaacgtcat cacgctcttg agttccccgt actgggcttc catgaacctg agcgcctcgg
     5581 ccgggccgcg atagctgttt tcccactggt tcttcgctgc gtactgcaga aactcctcgc
     5641 tcgaggcgat acgcttgaac acgttttccc agtaagcgac ctgcgccgcc gtcatgccct
     5701 tggtgccgat caccccgcgc cagttgtcga acacgccttt gtagccgagc tcgggccagg
     5761 tcgccgcggc tgcgagcggc ccctgcatac gcttcgacga cgtcaccgtc agcacgcgca
     5821 gctttccgct ctcgaccagg atggccgcgt tggcggtacc cccgttggcg acgtccacgt
     5881 gcccgccgag caatgccgtg aaggcctcgg ccgtggaatt gaaaccgacg agcttgacct
     5941 gcttgatgtc cacgcccgcc gactgcagtg gcaggccgac ggcgatgtgg tgcgtgccgc
     6001 cgatcgcgct gccgagtgcg atgctgaggg aggccgggtt cttgcgcaac gcctcgatca
     6061 tgtccttgcc ggtcttgagc ggagaatcgg agcgcaccac gaatgcgatg tgttccgtca
     6121 acagtaccgc aagcggcgtg aagtcggaat acgtcatcgg gatgcgcccg ttgatctggt
     6181 tggtcagtat ggttgaggac gtgatgctca ggtaatgcgg gtcgccggcg cgctgattga
     6241 gaaagttgta ggccatggca tgcccgccgc cggaacggtt ggccaccgtg ctcgacaccg
     6301 gcagcagttt gaggtcgtcc cagatcttgt gaacggtgcg tccggtcagg tcgagcgagc
     6361 cgccggcgct cgccggcacg atcagctcga cgttgcgctc gggcttccag ccttgcgcca
     6421 aggcggcg
//
LOCUS       DBNGWZ010000379         6804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_798405, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000379 DBNGWZ010000000
VERSION     DBNGWZ010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6804
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_798405"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /locus_tag="AC16CX_11125"
     CDS             complement(<1..1958)
                     /locus_tag="AC16CX_11125"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HIR3013097.1"
                     /translation="MNSNPPLLSVRGLRTWLDSGDAVVRAVDGVSFDIHHGETFALVG
                     ESGCGKSMTALSIMRLLPDAGSVVGGTVKLDGTDLLKLPETRMREVRGGRIGMIFQEP
                     ALSLNPVMTSGEQIAEALRRHTDLAGDALESRVLELLEAVHLPDAARRRHEYPFQLSG
                     GMKQRVMIAMALACDPELLIADEPTTALDVTIQAEVLELLRELQKTRRMSLMLITHDL
                     GVVAGMAHRVAVMYAGEIVETAARDAFFSRPAHPYSRKLFDSLPTAGKRDGALAVIRG
                     AVPSLAREFTGCRFAERCDSAWDLCRRSTPGLYDAGSGQIARCHLYAPGADKAEGRRQ
                     KAEVEVKAEGRRQKAEGQDQPRVTSHESLLQVKDLKVHFPVYGGLLKREVARVRAVDG
                     VSLAIEAGRTLGLVGESGCGKTTVGKGILRLTEPTGGAVLFDGVDLARLKRGELRPRR
                     KDAQIIFQDPYSSLNPRMRVMETLEEGMAALGIGDGREARQARVDALLAEVGLDPAVK
                     YRYPHEFSGGQRQRIAIARALSVEPRLIVCDEPTSALDVSVQAQILNLLRDLQRKRGL
                     AYLFISHNISVIEFLAHEVAVMYLGRIVEHGRVDEVLSDPKHPYTRALLSAVPVVDPG
                     TKREVIRLQGDLPSPVNPPAGCHFHPRCP"
     gene            complement(2103..3545)
                     /locus_tag="AC16CX_11130"
     CDS             complement(2103..3545)
                     /locus_tag="AC16CX_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013098.1"
                     /translation="MPFQIVVLWTDALVYVLVGAVGLFAWYVRRHEHLLVPWRKVGES
                     ASGVVGLTVLAAFVIVGLLDSLHFRPRLPAQERGAKAAYAVEVQSVLDLALGSLRARR
                     EKTYSAPLATELYAKETVDLAGGRQERVFPRLKHGGAHLADPGRDFAPDVTRRALWGL
                     AAGVLAWLAAAALLCAYLAWRGGVDIDTAWTRIWRGETTARWSAALITLAVIAACSGV
                     TIALSAKYFVFGTDKVGQDVLYQALKSVRTALVIGTLTTLVMLPFAIALGIMAGYFGG
                     WIDDVIQYVYTTLNSIPGVLLIAAAVLLVQLRIDLNPEAYPTSLERADLRLLWLCFIL
                     GITTWTGLCRLLRGEALKVKQLDYIQAALAFGVGHTRIITRHILPNVMHIVLISVVLE
                     FSGLVLAEAVLSYVGVGVDPSMNSFGTMINNARLEMAREPMVWWSLAAAFLLMVTLVL
                     AANLFADAVRDAFDPRIRAARRFRLAAQKA"
     gene            complement(3551..4531)
                     /locus_tag="AC16CX_11135"
     CDS             complement(3551..4531)
                     /locus_tag="AC16CX_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013099.1"
                     /translation="MLAYIVRRVLYAVPILVGVNVVTFALFFLVNTPDDMARMHLGVK
                     RVTPEAIAKWKQERGYDKPVLFNAAAPQLEGKFTQTIFFEKSLRMFAFDFGRADDGRD
                     IGREIRTRMVPSLALALPVFLVGLLVYVTFALGFAFFRATYIDFWGVVLCVAAMSISS
                     MFYIIGGQYLVSKLWHLTPISGFSGELDAARFLLLPAAIGVIAGFGGATRLYRTFFLE
                     EMSKDYVRTARAKGLPETGVLFRHVLKNAMIPILTGVVAAIPLLFIGSLVSESFFGIP
                     GLGSYVVDAINSQDFAIVRSMVFLGSVFYIFGLLLTDISYTLVDPRVRFE"
     gene            complement(4531..6699)
                     /locus_tag="AC16CX_11140"
     CDS             complement(4531..6699)
                     /locus_tag="AC16CX_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013100.1"
                     /translation="MLAFSHRAAVIAVALVAGCSGAPWNDPYPARESDANVLYSSFAE
                     RPKHLDPVQSYSSNEITFTAQIYEPPLQYHYLKRPYALTPLVAEAVPEPEYYDASGRR
                     LPADAAQRDIAHTVYEVRLKRGILYQPHPAFARTADGRPLYLGLGREGLAGRHQLGDF
                     KATGTRELKAQDFEYQIKRLAHPRLHSPIFGLMADYIVGLREYAEVLKRANQELTARA
                     GNGAWLDLSRYPLGGVEVLDDYTYRIRLHGQYPQFLYWLAMPFFAPVPIEVDRFFDQP
                     GMAERNLTLDWWPVGTGPYMLIENNPSSRMVLARNPNWRGETYPAEGEPGDALAGLLE
                     DAGKPLPFIERAVYSFEKEGIPRWNKFLQGFYDASGIASDTFDQAVQFSAQGDAIVTE
                     KMQRQGIRLSTSVAVSTFYVGFNMLDPVVGGLDERSRKLRQAISIAVDYEEYISIFQN
                     GRGIPAQGPIAPGIFGYREGATGINPVVYDWDGREARRKPIEAARKLLAEAGYPNGVS
                     ATTGEPLVLNFDVTARSAEAKSTLDWMQRQFEKINIQLRVRPTDYNRFQDKIRKGNTQ
                     IYEWGWHADYPDPENFLFLLHGPQSKVRSQGENASNYASPEFDRLFEQMKNMPNSPER
                     QRIIDRMLEIARRDAPWLWGYHPKDYALYHSWMKNVKANKIAYNTLKYQRLDPVARMQ
                     LRREWNRPVLWPLGLALIGLAIFTWPAVVSYRRREREAAR"
BASE COUNT         1219 a         2278 c         2164 g         1143 t
ORIGIN      
        1 ggacagcggg ggtggaagtg acaccccgcc ggcggattca ccggcgacgg caggtcgccc
       61 tgcagccgga tcacctcccg cttcgtcccc gggtccacca ccggcaccgc cgagagcaac
      121 gcacgcgtgt agggatgctt cggatcggag agcacctcgt ccacgcgacc gtgctcgacg
      181 atgcgcccga ggtacatgac cgccacttcg tgcgccaaga actcgatcac cgagatgttg
      241 tggctgatga agaggtaggc gagccctctt tttctctgaa gatccctcaa caggttcagg
      301 atctgcgcct gcaccgagac atcgagcgcg ctggtcggct cgtcgcacac gatgaggcgc
      361 ggctcgaccg acagcgcgcg cgcgatcgca atgcgctggc gctgcccgcc cgagaactcg
      421 tgcgggtagc ggtacttgac cgctggatcg agtcccactt cggcgagcag cgcgtccaca
      481 cgagcctggc gcgcctcgcg cccgtcgccg atcccgagcg cggccatgcc ctcctcgagg
      541 gtttccatca cgcgcatgcg cgggttgagc gaggaatacg gatcctggaa gatgatctgc
      601 gcgtccttgc ggcgcggccg caactcgccg cgcttgagcc gcgcgagatc cacgccgtcg
      661 aacagcaccg cgccgccggt cggctccgtg agccgcagga tgcccttgcc caccgtggtc
      721 ttgccgcagc ccgattcgcc caccagtccg agcgtccggc ccgcttcgat cgcgagcgag
      781 acgccgtcca ccgcccggac gcgcgccacc tcacgcttca ataagccgcc atagactgga
      841 aaatgaacct tcaggtcctt cacctgaagg agtgactcgt gactcgtgac tcgtggctgg
      901 tcctgtcctt ctgccttctg tcttctgcct tctgccttta cttccacttc tgccttctgc
      961 cttctgcctt ctgccttgtc cgcgcctggt gcgtagaggt ggcaccgcgc aatctgcccg
     1021 ctgccagcat cgtagagacc cggcgtggac ctgcggcaga ggtcccacgc ggaatcgcag
     1081 cgctcggcga atcggcagcc ggtaaactcg cgcgccagcg aaggcacggc tccgcggatc
     1141 acggcgagcg cgccgtcccg cttgcccgcc gtcggcagcg agtcgaagag cttcctcgaa
     1201 taagggtgcg cggggcggga gaagaaggcg tcgcgcgcgg cggtttccac gatctcgccg
     1261 gcgtacatca ccgccacccg gtgcgccatc cccgcaacca cacccaggtc gtgggtgatc
     1321 agcatcagcg acatgcggcg ggtcttctgc agctcgcgca gcagctccag cacctccgcc
     1381 tggatcgtga cgtcgagcgc ggtggtcggc tcgtcggcga tcaacagctc cgggtcgcag
     1441 gcgagcgcca tcgcgatcat gacgcgctgc ttcatgccgc ccgagagctg gaacggatac
     1501 tcgtgcctgc gccgcgcggc gtccggcagg tgcaccgcct ccagcagctc cagcacgcgt
     1561 gactccagcg catcgcccgc gaggtccgtg tggcgcctga gcgcttccgc tatctgctcg
     1621 ccggaggtca tcacgggatt caacgagagc gcgggttcct ggaagatcat gccgattcga
     1681 cccccgcgca cctcgcgcat ccgtgtctcc ggaagcttga ggagatcggt cccgtcgagc
     1741 ttaaccgtcc cgccgacgac gctgcccgcg tccggcagca gccgcatgat cgagagcgcg
     1801 gtcatggact tgccgcagcc cgattcgccc accagcgcga aggtctcgcc gtggtggatg
     1861 tcgaacgaga cgccgtccac ggctctcacg acagcatcgc cgctgtcgag ccaggttcta
     1921 agaccgcgga cgcttagtaa cgggggattg ctgttcatcc aatcccccga agcgagacac
     1981 gatgccccgt gacatggtga tgcgtgacgt gatacggcgt gacgtgatga tgcgacgatt
     2041 tgcaaacata gcgccaacca cgcttcacca tgtcacgccc taccatgtca cgctttacca
     2101 cgtcacgctt tctgggcggc aaggcggaag cgccgcgccg cccgtatgcg cgggtcaaag
     2161 gcatcgcgca cggcatcggc gaagaggttc gccgccagca ccagcgttac catgagcagg
     2221 aacgccgcgg ccagcgacca ccacaccatc ggctcgcgcg ccatctccag ccgggcgttg
     2281 ttgatcatgg tgccgaagct gttcatcgac ggatcgaccc cgaccccgac gtaggagagc
     2341 accgcctccg cgagaaccag cccgctgaac tccaacacta ccgaaatcag cacgatgtgc
     2401 atcacgttgg gcaggatgtg ccgcgtaatg atgcgcgtgt ggccgacccc gaaggcaagc
     2461 gccgcctgga tgtaatcgag ctgcttcacc ttcagcgcct ccccgcgcag caggcggcag
     2521 aggccggtcc acgtcgtgat gccgaggatg aagcaaagcc agagcaatcg caggtccgcg
     2581 cgctccagag acgtcggata cgcctcggga ttgaggtcga tccggagctg caccaggagc
     2641 accgcggcgg caatgagcaa cacgccggga atcgagttga gcgtggtgta tacatactgg
     2701 atcacgtcgt cgatccagcc cccgaagtag ccggccatga tgccgagcgc gatcgcgaac
     2761 gggagcatca cgagcgttgt caacgtgccg atcacgagtg cagtgcggac gctcttcagg
     2821 gcctggtaga ggacgtcctg gccgaccttg tcggtgccga agacgaaata cttcgcgctc
     2881 agcgcgatcg tcaccccgga gcacgccgcg attaccgcta gggtgatgag ggccgcgctc
     2941 caccgcgccg tggtctcgcc gcgccagatc cgggtccacg ccgtgtcgat gtcgaccccc
     3001 ccgcgccacg ccagatacgc gcagagcaag gcggcggccg ccagccatgc gagcactccc
     3061 gccgcaaggc cccacagagc gcgccgggtg acgtcggggg cgaaatcgcg ccccggatcg
     3121 gccagatgcg cgcccccgtg tttcagccgc gggaagaccc gttcctgccg tccgccggcg
     3181 agatccacgg tttccttggc gtaaagctcg gtggccagcg gagcggaata agtcttttcg
     3241 cgccgcgccc gcagcgaacc cagggcgaga tcaagcacgc tctgcacttc gaccgcgtag
     3301 gccgctttgg cgccgcgttc ctgggccggc aaacgaggcc ggaaatgcag cgagtcgagc
     3361 agccctacga ttacgaacgc cgcgagcacc gtgagcccca ccacgccgct cgcgctctcc
     3421 cccaccttgc gccacgggac cagcagatgc tcgtgacgcc ggacgtacca ggcgaagaga
     3481 ccgaccgccc cgaccaggac gtagacgagc gcgtcggtcc acagcacgac gatctggaaa
     3541 ggcatgtcgg tcactcgaac ctgacccgcg gatcgaccag cgtgtacgag atatccgtga
     3601 gcagcaatcc gaagatatag aacaccgagc ccaggaacac catcgaacgc acgatcgcaa
     3661 agtcctgcga gttgatcgcg tccaccacgt agctgccgag ccccggtatg ccgaaaaagg
     3721 actccgacac gaggctgccg atgaacagca gcggaatcgc cgccaccacg ccggtcagta
     3781 tcgggatcat ggcgttcttg aggacgtggc ggaacagcac gccggtctcc gggagtccct
     3841 tggcccgggc ggtgcgtacg tagtccttgg acatctcctc gaggaagaac gtgcggtaga
     3901 gccgggtcgc gccgccgaaa ccggcgatga cgccgatcgc cgcgggcagc agcaggaacc
     3961 gcgccgcgtc gagctcgccg ctgaatcccg agattggcgt gaggtgccac agcttgctca
     4021 ccaggtactg cccgccgatg atgtagaaca tgctggatat cgacatcgcc gccacgcaca
     4081 ggaccacgcc ccagaaatcg atgtaggtgg cgcgaaagaa cgcaaagccc agggcgaaag
     4141 tgacgtacac cagcagcccg accaggaaca cgggcagcgc gagcgcgagg ctcggcacca
     4201 tgcgggtgcg gatctcccgg ccgatgtcgc gtccgtcgtc cgcccgtccg aagtcgaacg
     4261 cgaacatgcg cagcgatttt tcgaagaata tcgtctgcgt aaatttcccc tcgagctgcg
     4321 gtgccgccgc attgaaaagg actggcttgt cgtagccgcg ctcctgcttc catttggcga
     4381 tcgcctcggg ggtcacgcgc ttgacgccga ggtgcattcg ggccatgtcg tctggcgtat
     4441 tgaccaggaa aaacagcgcg aacgtgacca cgttgacccc gacgagtatc gggaccgcgt
     4501 agagcacgcg ccgcacgatg taggccagca tcagcgggcg gcctcccgct cgcgccggcg
     4561 gtaggaaacg accgccggcc aggtgaagat cgccagcccg atgagcgcga ggccgagcgg
     4621 ccagagcacc gggcggttcc actccctccg caactgcatg cgcgcgaccg gatcgaggcg
     4681 ctggtacttg agcgtgttgt aggcgatctt gttggccttg acgttcttca tccacgagtg
     4741 gtacagcgcg taatccttgg gatggtagcc ccacagccag ggcgcatcgc gccgcgcgat
     4801 ctcgagcatg cggtctatga tccgctggcg ctcgggcgaa ttcggcatgt tcttcatctg
     4861 ctcgaacagg cggtcgaact cggggctggc gtaatttgac gcattctcgc cctggctcct
     4921 caccttgctc tgtggtccat gcagcaggaa caggaagttt tcggggtcag gatagtcggc
     4981 atgccatccc cattcgtaga tctgcgtgtt gcccttccgg atcttgtcct gaaagcggtt
     5041 gtagtcggtc gggcgcacgc gcagctggat gttgattttt tcgaactgcc gctgcatcca
     5101 gtcgagcgtc gacttggcct ccgccgagcg ggcggtcacg tcgaagttga gaacgagcgg
     5161 ctcgccggtc gtggcgctca cgccgttcgg gtagccggct tccgccagca gtttcctggc
     5221 ggcttctatc ggcttgcgcc gtgcttccct gccgtcccaa tcgtacacga ccggattgat
     5281 gccggtggct ccctcccggt agccgaagat gcccggcgcg atcggcccct gcgccggtat
     5341 gccgcgtccg ttctggaaga tggagatgta ctcctcgtaa tcgacggcga tcgagatcgc
     5401 ctgccgcagc ttccgcgagc gctcgtcgag gcctcccacc accgggtcga gcatgttgaa
     5461 cccgacgtaa aaggtggaca ccgccaccga cgtcgacagg cgtatccctt gccgctgcat
     5521 cttttcggtg acgatcgcgt cgccctgagc cgagaactgc accgcctggt cgaacgtatc
     5581 cgaggcgatc cccgatgcgt cgtagaagcc ctgcaggaac ttgttccagc gcggaatgcc
     5641 ttccttttcg aagctgtaga ccgcacgttc gatgaacggc agcggcttgc ccgcatcctc
     5701 gagcagcccc gccagcgcgt cgccgggctc gccttcggcc ggataggttt cgccgcgcca
     5761 gttgggattg cgcgcgagca ccatgcggct gctcggattg ttctcgatca acatgtaggg
     5821 cccagtccca accggccacc agtcgagcgt caagtttctc tccgccatgc cgggctggtc
     5881 gaaaaagcgg tccacttcga tcgggaccgg cgcgaaaaac ggcatggcga gccagtagag
     5941 aaactgcggg tactgcccgt gaagccgaat gcggtaggtg tagtcgtcga ggacctcgac
     6001 gccgccaagc gggtaacggc tcaggtcgag ccacgcgccg ttgcccgccc tggcggtcag
     6061 ctcttggttc gcgcgcttga gcacttcggc gtattccctc aggcccacga tgtaatcagc
     6121 catcagcccg aagatcggcg aatgcaaccg ggggtgtgca agccgcttga tctggtactc
     6181 gaagtcctgc gccttcagct cgcgcgttcc ggtcgccttg aaatcaccga gctggtggcg
     6241 gcccgcgagc ccctcgcgtc ccagcccgag gtagagcggc ctgccatcgg ccgtgcgcgc
     6301 gaacgccggg tgtggctgat aaaggatgcc gcgcttcagg cgcacctcgt agacggtatg
     6361 cgcgatgtcg cgctgcgcgg catcggccgg gaggcgcctg ccgctggcgt cgtaatactc
     6421 gggctcggga accgcctccg ccacaagcgg ggtcagcgca tagggccgct tgaggtagtg
     6481 gtactgcagc ggcggctcgt agatctgcgc ggtgaaggtg atctcgtttg agctgtagga
     6541 ctggacggga tcgaggtgct tcgggcgctc ggcaaaagag gagtacagga cgtttgcgtc
     6601 cgattcgcgc gccgggtacg ggtcgttcca gggtgcgccg ctgcatccgg cgacaagcgc
     6661 gaccgcgatc accgccgcgc ggtgggaaaa agccagcatg gcgaaaagtg tagcccattt
     6721 tacgaactgc gtgaccagta accagtaatc tgacaaggcc tgcgtttttg aagtcctccg
     6781 gtcaccggtt acgctactcg ttat
//
LOCUS       DBNGWZ010000380        10099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_297529, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000380 DBNGWZ010000000
VERSION     DBNGWZ010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_297529"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16CX_11145"
     CDS             <1..183
                     /locus_tag="AC16CX_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013101.1"
                     /translation="LREGGAPDLGEGVGVGAETGRSRRDYGTALFGELLSHWSRRRRS
                     QQGHRSLNRTRRSRYD"
     gene            180..1049
                     /locus_tag="AC16CX_11150"
     CDS             180..1049
                     /locus_tag="AC16CX_11150"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013102.1"
                     /translation="MIVPFAPGGGVDFIARLVGQKLAEALGQQIVVENRPGAGSAIGT
                     EVALRSAPDGYTLLEISPSYVINSALRPGKFDPTIDYTPIVLIGKGPFVVVAHPSLPA
                     RDARQLIALAKARPGEIVFGSSGQGTIVHLSTELFLYMAGVKMTHVPYKGGGPALIDV
                     MAGQVQLVFSPPQTGLPHVKAGRVRAIGMTSARRIPTEPGIPALAEAVPGYEATNWHA
                     MVGPKGLPRAVVDRINAEVRRIVAQAEMEKTLHASGVFPDGGGTPEQLSELVRRDFEQ
                     WRKVIVQAGVKAE"
     gene            complement(1182..2138)
                     /locus_tag="AC16CX_11155"
     CDS             complement(1182..2138)
                     /locus_tag="AC16CX_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3013103.1"
                     /translation="MKPEILVLTRIHPPTLAELEREFTVHRLWTTADPDALIREAAGS
                     VRGVVTNGLTGCSRGQIEALPRLEIIACLGTPHGTVDLAAARERGVIVTNTPDSITES
                     VADVAMGLLIAVMRRICENDRFVRAGKWLAGLPPLGRELGGKTCGIVGLGRIGNGVAR
                     RASACGMTVCYHGPRPKPEIAWTYFSDLEALARRSDCLVVTCTLTPATRGLVNARVLD
                     ALGPAGFLVNVARGPIVDQEALIAALRDKRIAGAALDVYWDEPRVPEDLIGMDNVVLT
                     PHIGSSTREVREARGAKVLANLRAHFAGKPVPHPFTGRSGEP"
     gene            complement(2249..2713)
                     /locus_tag="AC16CX_11160"
     CDS             complement(2249..2713)
                     /locus_tag="AC16CX_11160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3013104.1"
                     /translation="MKVKSTLKVINEKNYKGRRGVTKGQTYTRLVGWPEVFMTDRVRL
                     GRASYKPGSYEQLHWHPIEACYYVISGHATVRDINGKEYPVGPGSIIYAPPGIAGAHE
                     WKVKESLELIDIRATNETNRKMQFTVDKKTKRSYIELHELAYRDAISFKSHY"
     gene            complement(2853..3653)
                     /locus_tag="AC16CX_11165"
     CDS             complement(2853..3653)
                     /locus_tag="AC16CX_11165"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR3013105.1"
                     /translation="MDTPSMTEKLIATKDGAIGWVIFNNPQKRNAMSLDMYEATAAAL
                     EDYAKDPAIRVVILKGEGDKAFISGADISQFKDKRANLEQVRAADAISERCNQAIREC
                     PKPTIAMIRGYCMGGGVSIAVVCDLRIASEDSRFGIPAAKLGLGYRHAGVRRLMDLVG
                     PSFTAEIFYTGRQFSAQEALQMRLVNRVLPVAELEKYVLDCAATLAGNAPLTIAAVKR
                     SLIELRKDPNERDVALCQKMVDNCFASEDYKEGQAAFMEKRKPAFKGK"
     gene            complement(3685..4701)
                     /locus_tag="AC16CX_11170"
     CDS             complement(3685..4701)
                     /locus_tag="AC16CX_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3013106.1"
                     /translation="MINAAIVGLGRWGRNLVGSIQGRSEHLRFTQAVVRRPDAACDFA
                     RQHALELFADYGRVLADRSVQAIVLATPHSLHAEQIIAAAGAGKPVFCEKPLALKRAD
                     AVRAIDACRRAGVPLGLGYNKRFWPSMRELKRIVASGELGEILHVEGHFSNESTGRFY
                     TGWRGEDAESPAGGLTATGVHVLDAFVDLVGPARRVSAQMVARGSSPEPLDTLSVFIE
                     FANRASGVLACVRTTPQYWRIHVFGRNGSAEALEDTDLVVRKSDAPPRRLTFERVDAL
                     RLELEAFAHSVETGAPYPIPFGQMLDGVSVLEAITDSLETGSAVMLSEEGKSTSGRFR
                     RGNR"
     gene            complement(4750..5109)
                     /locus_tag="AC16CX_11175"
     CDS             complement(4750..5109)
                     /locus_tag="AC16CX_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013107.1"
                     /translation="MAITGNWIFTAAMDVDPDKEALFNEVYDTEHVPHLGKVPGVVSV
                     TRAKLDTLRVTMGGATKTVEPQAQGRPRYACYVELDNPDVLTSAAWAKAVDAGRWPTQ
                     VRPFTRNRQHTLHKVIK"
     gene            complement(5524..6072)
                     /locus_tag="AC16CX_11180"
     CDS             complement(5524..6072)
                     /locus_tag="AC16CX_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HIR3013108.1"
                     /translation="MARAESQALLSAQERLVDSYSSDHRFVADDIRGIHRAWLGSIYS
                     WAGEYRSVNVSKGGFHFAAAREVPRLMRDFEQRELANYTPCRFPAELEIVRALAVVHA
                     ELVLIHPFREGNGRCARLLALFMGFQAGLPPLDFGGWSGKVERPYVAAVHAAVGSNYQ
                     PMVDLFSEVIRQSYDRFGRRGR"
     gene            complement(6532..6831)
                     /locus_tag="AC16CX_11185"
     CDS             complement(6532..6831)
                     /locus_tag="AC16CX_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR3013109.1"
                     /translation="MAAKLLDPIPPGEILYEEFMKPLGVSINALAREIAVPPNRISEI
                     VSGKRAITADTALRLGKYFGVSPEIWLGLQADYDLRVAKRTTWPKTEPRVRAHAD"
     gene            complement(6840..7121)
                     /locus_tag="AC16CX_11190"
     CDS             complement(6840..7121)
                     /locus_tag="AC16CX_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR3013110.1"
                     /translation="MIKSFRSPDAEALFNDRPVVRFRAIERPARRKLLYLNQARSLRD
                     LLVPPGNRLEALKGDRKGQHSIRINDQWRICFRWTEGHAYDVKIVDYHD"
     gene            complement(7262..8491)
                     /locus_tag="AC16CX_11195"
     CDS             complement(7262..8491)
                     /locus_tag="AC16CX_11195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3013111.1"
                     /translation="MMRKLRTQRDNQQWMLDLALNMRGRVQNFERDDLEVPPGKRARN
                     YRMLPKVWREAAERHEKLARRADAMGARETATYHYDHAIEAYRMAQHPIYFDDHPVKI
                     ELHRKLSEMVDRRSKVASYPIERVEVPFDGGKTISCLLHLLPDRRRAPVVIYVPGMDQ
                     TKEVFPKAYHNIALSRGFHVLAMDGPGQGSSNMRKIRAVKDNYERAGAAVISYLQMRP
                     EVDPKRIAIYGISMGSYWSLRLSSYDHRAAAVVSAVACFNPDNTIFTQSSPRFKQMFM
                     YMAGYEDEAKFDREVASGMTVRGYLGKVQCPTLLVTGEFDPLCPLEDAVEAFHDLKVP
                     KEMWVFENQYHPLWGISNLGGMDTHDYVMDWLKGLFSGKRAPTKRGRIAYIREGGDGP
                     WGRCEWVPTIKPGQAYF"
     gene            complement(8606..9196)
                     /locus_tag="AC16CX_11200"
     CDS             complement(8606..9196)
                     /locus_tag="AC16CX_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3013112.1"
                     /translation="MKTISRSLLAALMAAASAALAQLDPLSIAGSVVTTTMDVRTKTE
                     VKNDVNIATSANKRLIDDKQAEWKGVTVLTFAQHVVLAGAVKSDDAKKLVEDIVRKDK
                     RIRSLVNDLVVIRKKGDEGDLVKDKVIDTKVNATLTATKGIASVNMRWKTVNGNVVLM
                     GAAQSEGEAKLAVSKIRELEGVKGVKNRLRIVPKKK"
     gene            9489..>10099
                     /locus_tag="AC16CX_11205"
     CDS             9489..>10099
                     /locus_tag="AC16CX_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013113.1"
                     /translation="MLRIVLQAVLSTFLALSSLGSIAQGYPSRPIRVVVPFTPGTGMD
                     IIARNVGPKLSERLGQPVVIDNRPGASGNLGAELVAKAAPDGYTMMVSGNPLVTSPHL
                     YRNVPYNPLTDFAPISLAAWGTLMLVANPGAKINSVADLISQAKANPGRINYGSPGIG
                     TPHHMSMELFKDLTGANLLHVPYKGSAGAVTDVLGGQINVMFMP"
BASE COUNT         1736 a         3309 c         3107 g         1947 t
ORIGIN      
        1 ttgcgcgagg gcggcgcacc ggatctcggc gagggggttg gtgttggggc ggagacgggg
       61 cgcagccgcc gggattatgg cactgcgctc ttcggcgagt tgctatcgca ctggtcgcgg
      121 cggcgtcgct cgcagcaggg gcatcgttcg ctcaaccgta cccgtcgaag ccggtacgat
      181 tgatcgttcc gtttgcgcca ggcggcggcg tggacttcat cgcccgcctg gtcgggcaga
      241 agctcgcgga agcgctgggg cagcagatcg tcgtcgagaa ccgcccgggg gcgggcagcg
      301 cgatcggcac cgaggtcgcc ctccgctccg cgccggacgg ctacacgctg ctcgagatct
      361 cgccgagcta cgtcatcaat tccgccctgc gcccgggcaa gttcgatccc accattgact
      421 acacgccgat cgtgctcatc ggcaagggcc ccttcgtcgt cgtggcgcac ccgtcgctgc
      481 ccgcccgcga cgcgcggcag ctgattgcgc tcgcaaaagc gagaccgggc gagatcgtct
      541 tcggcagcag cgggcaaggc accatcgtgc acctttccac cgagctgttc ctgtacatgg
      601 cgggcgtgaa gatgacgcac gtcccgtaca agggcggcgg cccggcgttg atcgatgtca
      661 tggcgggcca ggtgcaactc gtcttctcgc cgcctcaaac gggacttccg cacgtgaaag
      721 ccggccgcgt gcgcgcgatc ggcatgacga gcgcgcggcg cattcccacc gagcccggca
      781 tcccggcgct cgccgaggcg gtgcccggct acgaggccac caactggcac gcgatggtcg
      841 gtccgaaggg cctgccgcgc gcggtggtgg accgcatcaa cgccgaggtg aggaggatcg
      901 tggcgcaggc ggaaatggaa aaaacgctgc acgcgagcgg cgtcttcccc gacggcggcg
      961 gcacgcccga gcagctctcc gaactcgtcc gcagggattt cgagcagtgg cggaaggtca
     1021 tcgttcaggc aggagtcaaa gctgagtgaa gcgtgatagg tgataggtga taggtgatag
     1081 gtgataggaa ttttaggccg gcggcgccag ccgctcttat cacttatcac gccgttcgag
     1141 tcctatcact tatcactcgt cacttatcac gcatttaagg cttatggctc gccgctcctt
     1201 cccgtgaacg ggtgggggac cggtttcccg gcgaaatgcg cgcgcaggtt cgccagcacc
     1261 ttcgccccgc gcgcctcgcg gacctcgcgg gtggatgagc cgatgtgcgg ggtaagcacg
     1321 acgttgtcca tgccgatcag atcttccggc acgcgtggct cgtcccaata gacgtcgagc
     1381 gccgccccgg cgatccgctt gtccctgagc gccgcaatta gcgcctcctg gtccacaatc
     1441 ggcccgcgcg cgacattcac gaggaagcct gccgggccca gcgcatcgag cacccgcgcg
     1501 ttgaccaggc cgcgggtcgc gggtgtcagc gtgcatgtca cgaccaggca atcggaacgc
     1561 cgcgccagag cttcgagatc ggaaaaatac gtccaggcga tctccggctt cggcctcggc
     1621 ccgtggtagc acacggtcat cccgcaagcc gaagcccgcc tcgcgacacc gttcccgatc
     1681 ctcccgaggc cgacgatgcc gcaggtcttc ccacccagct cgcggcccag cggcggcaac
     1741 ccggccagcc atttcccggc gcgcacgaag cggtcgttct cgcagatgcg gcgcatgacc
     1801 gcgatgagca ggcccatcgc cacatccgcc acggactccg tgatggaatc cggcgtgttc
     1861 gtcacaatca ccccgcgttc cctcgccgca gcgaggtcca ccgtgccgtg cggcgtgccg
     1921 aggcaggcga tgatctccag ccggggcagg gcctcgatct ggccgcggct gcacccggtc
     1981 agaccgttgg tcaccactcc gcgcacgctt ccggcggcct cccggatcag cgcgtccgga
     2041 tcggctgtgg tccacagcct gtgcacggtg aactcacgct cgagctcggc gagcgtcggt
     2101 ggatggatcc gcgtgagcac taaaatttct ggcttcatgt cctcatccag aaattggtga
     2161 taggtgataa gtgataggtg ataggagcca aatcccgcgc gagcgcgggt accttatcac
     2221 ttatcactcg tcacttatca ctcaaatctc agtagtgaga tttgaagctg atcgcgtcgc
     2281 ggtaagcgag ctcgtgcagc tcgatgtagg agcgcttggt cttcttgtcc accgtgaact
     2341 gcatcttgcg gttggtctcg ttggtggcgc ggatgtcgat cagctccaag ctctccttga
     2401 ccttccattc gtgggcgccc gcgatgccgg gcggcgcgta gatgatggag ccgggaccga
     2461 cggggtattc cttgccgttg atgtcgcgca cggtggcgtg tccggaaatc acgtaatagc
     2521 aggcctcgat cggatgccaa tgcagctgct cgtagctccc gggcttgtag ctcgcccggc
     2581 cgagccggac ccggtccgtc atgaacactt ccggccaccc gaccagccgc gtataggtct
     2641 ggcccttggt cacccctcgc cggcccttgt agtttttctc gttgatcacc ttcaacgtcg
     2701 atttcacctt catcggcctg cctccttaaa tgcgtgatgg gtgatgggat acccgaagga
     2761 tgagggatga aggatgaggg atgacccccg cagtgcggga gtcaaaacca ggtcatcctt
     2821 ccgccttccg ccttcatcct tcagtttgcc cgctacttac ccttgaaagc tggcttccgt
     2881 ttttccatga aggccgcctg cccttccttg tagtcctcgc tcgcgaagca gttgtccacc
     2941 atcttctggc acagcgcgac gtcgcgctcg ttcgggtcct tgcgcagctc gatcaggctg
     3001 cgcttcaccg cggcgatcgt gagcggggca ttgccggcga gcgtggcggc gcagtccaac
     3061 acgtatttct ccagctcggc gaccggcagc acgcggttca caagccgcat ctgcaaggcc
     3121 tcctgcgcgc tgaactggcg tcccgtgtag aaaatctcgg ccgtgaacga cgggcccacg
     3181 agatccatca gccgcctcac gccggcatga cgatatccca ggccgagctt cgccgcgggg
     3241 atgccgaagc gcgagtcctc cgacgcgatg cggagatcgc agaccacggc gatgctgacg
     3301 ccgccgccca tgcagtagcc gcggatcatg gcgatcgtcg gcttggggca ttcgcggatc
     3361 gcctggttgc agcgctcgga gatggcatcg gccgcacgca cctgctcgag gttcgcgcgc
     3421 ttgtccttga actgcgagat gtccgcgccg gaaatgaacg ccttgtcgcc ctcgcctttc
     3481 aggatcacga cgcggatcgc gggatccttc gcgtaatctt ccagcgccgc ggcggtcgct
     3541 tcgtacatgt cgagcgacat ggcgttgcgc ttctgcgggt tgttgaagat cacccagccg
     3601 atcgcgccgt ccttcgtggc gatcagtttt tcggtcatgg atggcgtgtc catgtgcggt
     3661 ctccgacccg taattcggct cattctacct gttgccgcgg cggaatcgtc ccgaagtcga
     3721 tttcccctcc tcgctcagca tcacggcgct cccggtctca agcgaatccg tgatcgcctc
     3781 cagcaccgag acgccgtcca gcatctgccc gaacgggatc ggatacggcg cgccggtctc
     3841 gacgctgtgc gcaaatgctt ccagctccag gcgcaacgcg tcgacgcgct cgaaggtcag
     3901 ccgccggggc ggcgcatcgc tcttgcgcac cacgagatcg gtgtcctcca gggcttccgc
     3961 cgagccgttc ctgccaaaaa cgtgaatgcg ccagtactgc ggcgtggtcc ggacgcaggc
     4021 gagcacgccg ctcgcgcgat tcgcgaactc gatgaagacc gagagcgtgt cgagcggctc
     4081 cgggcttgat ccgcgggcca ccatctgcgc ggacactcgg cgcgcgggtc cgaccaggtc
     4141 gacgaaggca tcgagtacgt gcacgccggt agccgtgagc ccgccggccg gtgattccgc
     4201 gtcctcgccg cgccagccgg tatagaaccg gccggtgctc tcgttgctga aatgcccttc
     4261 cacgtgcagg atctcgccga gctcgccgct cgccacgatc cgcttgagct cgcgcatcga
     4321 cggccagaag cgcttgttgt aacccagtcc cagcggaacg ccggcgcgcc ggcaggcatc
     4381 gatcgcgcgc acggcatcgg cccgtttgag cgccaggggc ttctcgcaga acaccggctt
     4441 gcccgctccg gccgccgcga tgatctgctc cgcgtgcagc gagtgcggtg tggccagcac
     4501 gattgcctgt acgctcctgt cggccagcac gcggccgtaa tcagcgaaca gctcgagggc
     4561 atgctgtctg gcgaagtcgc aggccgcgtc aggccgtctg accactgcct gagtaaaccg
     4621 cagatgctcg ctcctgccct ggatcgagcc gacgaggttc cgtccccacc ggccgagacc
     4681 gacgattgcc gcgttgatca tctctcagtg agtgaggaaa aggagtgaca agtgcgttac
     4741 tccctcccgt cacttgataa ccttgtgaag cgtatgctgc ctgttccgcg taaagggtcg
     4801 gacctgcgtc ggccagcgtc ccgcatcgac cgctttcgcc cacgcggcgc tggtgagcac
     4861 gtccgggtta tcgagctcga cgtagcaggc atagcggggc ctgccctgcg cctgcggctc
     4921 gacggtcttg gtcgccccgc ccatcgtgac gcgcaacgtg tcgagcttcg cccgcgtcac
     4981 cgagaccacg cccggcacct tgccgaggtg cggaacgtgc tcggtgtcgt acacctcgtt
     5041 gaacagggcc tccttgtccg gatcgacgtc catggcggcg gtgaagatcc agttgccggt
     5101 gatggccatg cgtttctctt tcttccttga acggctccag ggcgagcatc ctaacaggaa
     5161 aggaaaaata agacttttaa accgagcctg gtaaattcag gggtggcccg tgaaggggcg
     5221 gctcaggggt cccaacaaca gaaacggcgc taatcgtgat tcgtaagccg tgattcgact
     5281 cgcaatactt tcctcctgtc gaaggaggga tgggcaggtc accacctcat tcaaggctcg
     5341 catccttcag gagtataatt gagcctatgg gttcgaagct ttccgacgtc aatccctact
     5401 tgcgcgatcc cgcaattcgc aaggaaggct tgtgggtctc cgctaagacc tcttccgcga
     5461 tcgagggcat ccacgagcct tttgccaagc ggaatattgc tgcggtctcg cgcaagcgca
     5521 gctctaacgt ccgcgccgcc cgaaacggtc gtaagactgg cggatcactt cgctgaataa
     5581 atcgaccatc ggctggtagt ttgagccgac tgcggcatgc acggccgcga cgtagggtcg
     5641 ttccaccttc cccgaccaac cgccgaagtc gagcggcggc aaaccggctt gaaaccccat
     5701 gaacaaggct agcagccggg cgcagcggcc gtttccctcg cggaatggat gaatcagcac
     5761 caactcggcg tgcacgacgg caagtgcccg cacgatttcc aattccgcgg gaaaccgaca
     5821 aggagtgtaa ttcgcaagct cccgttgctc gaagtcacgc atcaatcgcg gcacttccct
     5881 tgcggcggcg aagtgaaacc cacctttgga aacgttcacg ctgcgatact ctcctgccca
     5941 ggaatagatg cttcccagcc aagcacgatg gatcccgcga atgtcatcgg cgacaaaacg
     6001 atggtcggac gaataactgt caactaatcg ttcctgggcg ctcaggagag cctgcgactc
     6061 ggccctcgcc atcgcccgca tcgagcggat tccaagcagg tttcgaagaa cgcggttgcg
     6121 agaccccggc tcgaactccg cctccgggcc gtgcgccgcg tagcggccag tgttacgagt
     6181 cacctatcac ctatctggaa aggcgtgagc cgtgattcgt gaatcgtgat tcgactggtc
     6241 ctcttatcgg ccttcttccc cgcttcttca tccctcaaga aatcaatcct caatcgattt
     6301 ctttccggct gctcggcggc gaacaggggc cccgaaagaa atggtcgggg ttatctcgcc
     6361 gagctgcggt cgttccagta tacgtccgct aatcgcgaga cgtaggcatc caccaactgc
     6421 ggcgtctgca gaaggtagcc agcgaattga tgaaacattc tagttttgct actggggccg
     6481 ccccccgaca acgcgcgacc ccgcgcgttg cgtgcttccc gcgaacctcc ttcagtctgc
     6541 gtgcgcccgc acccgcggct cggtcttcgg ccaggtcgtg cgcttcgcga cgcgcaaatc
     6601 gtagtcggct tgcaggccca gccagatttc cggggagacg ccgaaatact tgccaagccg
     6661 cagcgccgtg tcggcggtga tcgcgcgctt gccgctgacg atctcgctga tgcggttggg
     6721 cggcacggcg atctcgcgcg cgagcgcgtt gatgctcacg ccgagaggct tcatgaattc
     6781 ctcgtagagg atttcgccgg ggggaatcgg atcgagcagt ttggcggcca tggtcgctcc
     6841 taatcgtggt aatcgacgat cttgacgtcg tacgcgtgtc cttcggtcca gcggaagcag
     6901 atccgccact ggtcgttgat ccggatgctg tgctggccct tgcggtcgcc cttgagcgct
     6961 tcgagacgat tgcccggagg aacgagcaaa tcccggagcg agcgcgcctg attcaggtaa
     7021 agaagcttgc gccgcgccgg ccgctcgatc gctcgaaagc gcacaaccgg acgatcgttg
     7081 aacagcgctt ccgcgtcagg cgaccggaag gacttgatca cgcggcgagg gtattacgtc
     7141 tcgcgtaata cgtcaagcgg aaggcgccgg ccccgccggc tcaccctcag gtaggagggg
     7201 gattttctgg gagagccacc cccatcccaa ccttccccct gagggggaag gagggtggtg
     7261 gctagaagta ggcctgccca ggtttgatgg ttggaaccca ttcgcacctg ccccatggcc
     7321 cgtcgccgcc ctcccggata taggcgatgc ggccgcgctt cgtcggcgcg cgcttgcccg
     7381 agaacagccc cttcagccag tccatgacgt agtcgtgggt gtccatgccg cccaggttcg
     7441 aaatgcccca cagcgggtgg tactggttct cgaacaccca catctccttc ggcaccttga
     7501 ggtcatggaa cgcctcgacc gcgtcctcga gcgggcacag cggatcgaac tcgccggtga
     7561 cgagcagcgt cgggcattgc accttgccga ggtagccgcg gacagtcatg ccgctcgcca
     7621 cttcccggtc gaacttcgcc tcgtcctcgt agcccgccat gtacatgaac atctgcttga
     7681 agcggggcga ggactgcgtg aagatggtgt tgtccgggtt gaagcacgcg actgcgctca
     7741 ccaccgccgc ggcgcggtgg tcgtagctcg agagccgcag cgaccagtag ctccccatgc
     7801 tgatgccgta gatcgcgatc cgtttcggat ccacctcggg ccgcatctgt agatagctga
     7861 tcaccgcggc gcccgcgcgc tcgtagttgt ccttcaccgc gcggatcttc cgcatattgg
     7921 agctgccctg ccccgggccg tccatcgcga gcacgtggaa gccgcgcgag agcgcgatgt
     7981 tgtggtaggc cttgggaaag acctccttgg tctggtccat cccgggcacg tagatgacaa
     8041 cgggagcccg ccgccggtcg ggcagcaggt gcagcaggca cgagatggtc ttgccgccgt
     8101 cgaacggcac ctcgacgcgc tcgatcgggt aggacgcgac cttcgagcgg cgatccacca
     8161 tctcggacag ttttcgatgc agctcgatct tgaccggatg gtcgtcgaag tagatcgggt
     8221 gctgcgccat gcggtaggcc tcgatcgcgt ggtcgtaatg gtaggtcgcg gtttccctgg
     8281 cacccatggc gtccgcgcgc cgggcgagct tttcgtgccg ctccgccgct tcgcgccaca
     8341 ccttgggcag catgcggtag ttgcgcgcgc gcttgcccgg cggcacctcg aggtcgtcgc
     8401 gctcgaagtt ctgcacccgc ccgcgcatgt tgagcgcgag gtcgagcatc cactgctggt
     8461 tatcgcgttg ggtcctcagc ttgcgcatca tcggtttccc tcccaaaagc gtgataggtg
     8521 ataggtgata ggtgatagga gtttaaggcc ggcaacgccg gccgttctaa tcacttatca
     8581 ctcgacactc gtcactcgac actcgtcact tcttcttggg cacaatcctg aggcggttct
     8641 tgacgccctt caccccctcg agttccctga tcttggacac ggcgagcttc gcctcgcctt
     8701 cggactgcgc cgctcccatc agcaccacgt tgccgttgac cgtcttccag cgcatgttga
     8761 ccgatgcgat gcccttggtc gcggtgaggg tggcgttgac cttggtgtcg atcaccttgt
     8821 ccttcaccag atcgccttcg tcgcctttct tgcggataac gacgagatcg ttcacgagcg
     8881 aacggatacg tttgtccttc ctgacgatgt cctcaacgag ctttttcgca tcgtcggact
     8941 tgaccgcgcc ggcgaggacg acgtgctggg cgaaggtgag gaccgtgacc cccttccatt
     9001 ccgcttgctt gtcgtcgatc aggcgcttgt tcgccgaggt cgctatgttc acgtcgttct
     9061 tgacctcggt cttggtgcgc acgtccatcg tcgtggtcac caccgagccg gcgatggaga
     9121 gcggatcgag ctgcgccagc gcggccgatg ccgccgccat cagtgctgcc agcaatgatc
     9181 tggaaatggt cttcatcgat ttcccaccca aatgcgtgat aagtgacggg tgataagtga
     9241 taggactcga accgcgtgat aagtgataag tgataagagc ggccggcact gccggcctta
     9301 actcctgtca cctatcacct atcacgcttt ttggcctcag ctcctgtcac ccatcactta
     9361 tcacccttca cgcctcttcg ctcgccgata tgctacctcc aaagaacgaa cacacggtgt
     9421 ggaaaatttg cgaaaatgct tcacccgtcc accatccgag acgggggttg cccgcggagg
     9481 agtcgtccat gctgcgcatt gttcttcaag ccgtcctttc aacttttctg gcattgtcga
     9541 gcctgggctc catcgcccaa ggttatccgt cgcggccgat ccgggtcgtc gttccgttca
     9601 cgcccggcac cgggatggac atcatcgccc gcaacgtcgg gccgaagctc tcggagcgcc
     9661 tcggacagcc ggtggtgatc gacaaccggc cgggggcgag cggcaatctc ggcgccgagc
     9721 tggtcgccaa ggcggcgccg gacggttaca cgatgatggt atcggggaac ccgctcgtca
     9781 cgtctcccca tctctaccgg aacgtcccgt acaacccgct gaccgacttc gcgccgattt
     9841 cgctcgccgc gtggggcacg ctgatgctgg tcgccaaccc cggggcgaag atcaacagcg
     9901 tggccgacct catttcccaa gcgaaggcca atccgggcag gatcaactac ggctcgcccg
     9961 gcatcggcac gccgcaccac atgtcaatgg agctgttcaa ggatctcacc ggcgcgaacc
    10021 tcctgcacgt gccgtacaag ggcagcgcgg gcgcggtcac cgacgttctc ggcgggcaga
    10081 tcaacgtgat gttcatgcc
//
LOCUS       DBNGWZ010000381         4352 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_551215, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000381 DBNGWZ010000000
VERSION     DBNGWZ010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_551215"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="AC16CX_11210"
     CDS             <1..617
                     /locus_tag="AC16CX_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3013114.1"
                     /translation="HARASAPVSITPLMLSQLEGVLTRERHPLQSTDFSFSRFLTPHL
                     SGYAGWSVFMDCDMLVLDDIANLYRLLDDRYAVMVEKHHHVPKETVKFLGEPQSKYEK
                     KNWSSVMLFNNARCRALTPEYVNQASGLELHQFKWLASDELIGALPDRWNHLVGYSPP
                     RKDASLVHYTLGGPYFDDYRDCEYSKEWFAERDAMLRVASRAMA"
     gene            complement(614..2329)
                     /gene="soxB"
                     /locus_tag="AC16CX_11215"
     CDS             complement(614..2329)
                     /gene="soxB"
                     /locus_tag="AC16CX_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HIR3013115.1"
                     /translation="MSITRREFLQMLAVAAAHGMPLDGRAADTARGEVIYDLPQASGN
                     VTLMHFTDCHAQLLPAYYREPDVNLGVNDAWGKPPHLVGEALLKHFKIAPKSRFAHAY
                     THLDFERAARRYGKVSGFAHLATLVKRVRAQRPGALLLDGGDNWQGSATALWTKAQDM
                     VEVSKLLGVDMMTGHWEFTFGAGRVKEVLARDFAGRIEFVAQNVTTADFGDPVFKAYM
                     MREVNGVPVAIVGQAFPYTPIAHPRHFVAEWTFGIQEENLQKVVDEVLRKGAHAVVLL
                     SHNGMDVDLKLASRVTGIDAILGGHTHDAVPDPVTVRNARGATLVTNAGCNGKFLAVL
                     DLEVRGGAVKGHQYRLLPVFSNLLEPDQEMAALITKLRAPYEKQLAEKLAVTESLLYR
                     RGNFTGTFDQVILDALMEVKDAEIAFSPGFRWGTTILPGQAITMEHVMDQTAVTYPYT
                     TVTEMSGETIKNILEDVCDNLFNPDPYYQQGGDMVRVGGMRFSVDPTRGIGGRISNMT
                     LGGKPVGARKTYKVASWAPVAEGASGEPVWEVVSRYLRAKKVVRSVQPNRPALTGVQG
                     NPGLS"
     gene            complement(2448..3089)
                     /gene="soxX"
                     /locus_tag="AC16CX_11220"
     CDS             complement(2448..3089)
                     /gene="soxX"
                     /locus_tag="AC16CX_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR3013116.1"
                     /translation="MRKKRWLAWAMSVAPVLIVAGCASFPDEATTRQIAEKMMGEAYS
                     GTSPAFSGRVVQDRSQQICSKIGNAKLTQQESAEVVRLARSSIKYPASGKLVGDWRVG
                     AKLAYNGQGSRILGGKLEKSKENGALCSNCHQLDPKEFNVGNIGPSLTGYGAQRGNSE
                     AVAKLTYERIYNAWAHSPCSNMPRLGSSGHLTPEQIANVVAYLVDPQSPVNRK"
     gene            complement(3108..3920)
                     /gene="soxA"
                     /locus_tag="AC16CX_11225"
     CDS             complement(3108..3920)
                     /gene="soxA"
                     /locus_tag="AC16CX_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR3013117.1"
                     /translation="MPITRGGVAAILLACLAAGGAVLAQEKPGDGLAIGRQMLAEDNP
                     GELWVELGKRLFYEKRGPKNASLEQCDFGLGPGKLEGAYAQLPRYFPDTDKVQDLESR
                     LVTCMITLQGLKREDLDKTAMGNLDRMSDTEALVAFIASRSNGMKMNVRLSHQKEYEM
                     YKAGEYIFFRRNGPTDFGCINCHGENGKRIRLQDLPNMTDRQQLQAVVSTWPAYLGTQ
                     SNLRTMQWRLKICYWQMRLPDLAYLSDVTVAITSYLNYQGNDAVISVPGVKR"
     gene            complement(3961..4281)
                     /gene="soxZ"
                     /locus_tag="AC16CX_11230"
     CDS             complement(3961..4281)
                     /gene="soxZ"
                     /locus_tag="AC16CX_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR3013118.1"
                     /translation="MAEPMKIRATLQGDVADVRILMLHPMETGQRKDSKGEIVPLHFI
                     QSVVITHNGRTVLDAQWSQAVSRNPFLGLRIKGAKAGDKISVTWVDNKGDRRTDEATV
                     AAAS"
BASE COUNT          702 a         1417 c         1370 g          863 t
ORIGIN      
        1 tccacgcgcg cgcttccgcg ccggtctcga tcacaccgct catgctgtcc cagctggaag
       61 gagtgctcac ccgcgagcgg caccccctgc agagcacgga tttctctttt tcgcgcttcc
      121 tcacgccgca cctgtcgggg tatgcgggat ggtcggtgtt catggactgc gacatgctgg
      181 tgctcgacga catcgccaat ctctaccggc tgctcgacga ccgctacgcg gtcatggtgg
      241 agaagcacca ccacgtgccg aaggaaaccg tgaagttcct cggcgagccg cagagcaagt
      301 acgagaagaa gaactggtcg agcgtgatgc tgttcaacaa cgccaggtgc cgggcgttga
      361 cgcccgagta cgtgaaccag gcctcggggc tcgagctgca ccagttcaaa tggctggcga
      421 gcgacgagct gatcggcgcg ctgccggacc gctggaacca tctcgtgggt tacagcccgc
      481 cgcgcaagga cgcctccctc gtgcactaca cgctgggcgg accgtacttc gatgactacc
      541 gggattgcga gtactcgaag gagtggttcg ccgagcgcga cgcgatgctg cgcgtggcga
      601 gccgggccat ggcctaggaa agccccgggt tgccctgcac gccggtgagc gccgggcggt
      661 tgggctgcac cgagcgcacc accttcttcg cgcgcaggta gcgggaaacc acttcccaga
      721 ccggctcgcc gctcgcgcct tccgcaaccg gcgcccagct cgccaccttg tatgtcttgc
      781 gcgctccgac cggcttgccg ccgagcgtca tgttgctgat gcggccgccg atcccgcgcg
      841 tgggatcgac gctgaatcgc atgccgccca cgcgcaccat gtcgccgccc tgctggtagt
      901 aggggtccgg gttgaacagg ttgtcgcaca cgtcctcgag gatgttcttg atcgtttccc
      961 cgctcatctc cgtaacggtg gtataggggt aggtgaccgc tgtctggtcc atcacgtgct
     1021 ccatcgtgat ggcctggccg gggaggatcg tcgtgcccca gcggaatccc ggtgagaagg
     1081 cgatttcggc gtctttcact tccatcagcg cgtccaggat gacctggtcg aacgtgccgg
     1141 tgaagttgcc acgccgatag agcagactct cggtcaccgc gagcttttcg gcgagctgct
     1201 tctcgtacgg cgcccgcagc ttcgttatga gggcggccat ctcctggtcg ggctcgagga
     1261 ggttggaaaa caccgggagc agccggtatt gatggccttt cacggccccg ccccggacct
     1321 ctaggtccag caccgcgaga aacttgccgt tgcagccggc gttggtgacc agcgtggcgc
     1381 cccttgcgtt cctcacagta acgggatcgg gtactgcgtc gtgcgtgtgg ccgccgagga
     1441 tcgcgtctat gccggtcacg cgcgaagcga gcttgagatc cacgtccatg ccgttgtgcg
     1501 agagcagcac cacggcgtgc gcacctttcc tgagcacctc gtccaccacc ttctgcaggt
     1561 tttcttcctg gatgccgaag gtccattccg ccacgaagtg ccgcgggtgg gcgattggcg
     1621 tatagggaaa tgcctggccg acgatcgcga ccgggacgcc gttcacctcg cgcatcatgt
     1681 aggccttgaa caccgggtcg ccgaaatcgg cggtcgtgac gttctgcgcg acgaactcga
     1741 tcctgccggc gaagtccctg gcgaggactt ccttgacgcg cccggcgccg aaggtgaatt
     1801 cccagtgacc ggtcatcatg tccaccccga gcagcttcga gacctcgacc atgtcttggg
     1861 ccttggtcca cagtgcggta gctgagccct gccagttgtc gccgccgtcc aagagcaacg
     1921 cgcccggccg ttgcgcacgc acccgtttca ccagcgtagc caggtgcgca aagccgctga
     1981 ccttgccgta gcgacgtgcc gcccgctcga aatcgagatg agtgtaggca tgcgcaaacc
     2041 gcgatttcgg ggcgatcttg aagtgcttga gcagcgcctc gcctaccaga tgcggcggct
     2101 tgccccaggc gtcgttgacg ccgagattga cgtccggctc gcggtagtaa gccggcagca
     2161 gttgagcgtg acagtcggtg aaatgcatca gggtgacgtt gccgctggct tgcggcaggt
     2221 cgtagatgac ctcgccgcgc gccgtgtccg ccgcgcgccc gtcgagcggc atgccgtggg
     2281 ccgccgccac cgcgagcatt tgcagaaatt cacggcgagt gatggacatt accccagcct
     2341 ggcaacggaa atgtcgatga gatgatcagc gattgccgcg cgggtcgggc ggcgaccgcg
     2401 cccccgctcc agcgggcgca aatcaagcgg cgctttcaac gaccacgtta tttccggttt
     2461 accggcgatt gcggatccac gaggtacgcc acgacgttgg caatctgctc gggcgtgagg
     2521 tgcccgcttg agccgagccg cggcatgttg gagcaggggg agtgtgccca tgcattgtat
     2581 atccgttcgt aagtgagctt cgcgaccgct tccgaattgc cgcgctgcgc gccatacccg
     2641 gtcaggctcg gtccgatgtt gccgacgttg aattccttcg ggtcgagctg gtggcagttg
     2701 gagcagagcg cgccgttctc cttgctcttt tcgagcttgc cgccgagaat gcggctccct
     2761 tggccgttgt aggcgagctt cgcgcccacc ctccagtcac ccacgagctt cccggaggcc
     2821 gggtacttga tcgaggaccg cgcgagcctg accacctcgg cagattcctg ctgggtgagc
     2881 ttcgcattgc cgatcttgct gcagatctgc tgggaccggt cctgaaccac acgcccggaa
     2941 aatgctggcg acgtgccgga gtacgcctcg cccatcatct tctccgcgat ttgccgcgtc
     3001 gtcgcctcgt cgggaaagct cgcgcagccc gcgacgatca acacgggagc gacggacatc
     3061 gcccacgcca gccatctttt tttgcgcatt gtctcctcct ccgcccctca gcgtttcacg
     3121 ccgggcaccg agatgacggc gtcgttgccc tgatagttga gatagctggt gatggcgacc
     3181 gtgacatcgg acaggtaggc gaggtcgggc aggcgcatct gccagtagca gatcttgagt
     3241 cgccattgca tggtgcgcag gttgctctgc gtcccgagat acgccggcca ggttgaaaca
     3301 accgcctgca gttgctggcg gtcggtcatg ttcggcaggt cctgcagccg gatgcgcttg
     3361 ccgttttcgc cgtggcagtt gatgcagcca aaatcagtcg ggccgttgcg gcgaaaaaaa
     3421 atgtactcgc ccgccttgta catctcgtac tccttctgat ggctcaggcg cacgttcatt
     3481 ttcatcccgt tggagcggga ggcgatgaaa gcgaccagcg cttccgtgtc ggacatacgg
     3541 tcgaggttgc ccatcgcggt cttgtcgagg tcttcgcgct tgagtccctg cagggtgatc
     3601 atgcaggtta ccagccgcga ctccaggtcc tgcaccttgt cggtgtcggg gaaataacgc
     3661 ggcagttgcg cgtatgcgcc ctcgagcttt ccaggtccca gcccgaagtc gcactgttcc
     3721 agcgacgcat tcttgggccc gcgtttctcg tagaaaagcc gcttgccgag ttccacccac
     3781 agttcgcccg gattgtcctc ggcgagcatc tggcgcccga tcgccagtcc gtcaccgggc
     3841 ttttcctgtg cgagcaccgc accccctgct gcgaggcacg caagaagaat ggcggcaacg
     3901 ccgccgcgcg ttatcggcat gatttcctcc tcctgctgtt accggcccgg ccggcggcca
     3961 tcacgacgcc gcggccacgg tcgcttcgtc ggtgcgccgg tcgcccttgt tgtccaccca
     4021 ggtgacgctg atcttgtctc ccgccttggc gcccttgatg cgcagcccga ggaacgggtt
     4081 gcgcgaaacc gcctggctcc attgcgcgtc gagcacggtc ctgccgttgt gggtgatcac
     4141 cacgctctgg atgaagtgca gcggcacgat ttcgcccttg gagtccttgc gctgccccgt
     4201 ctccatcggg tgcagcatca ggatgcgcac gtcggcgacg tcgccttgca gcgtagcgcg
     4261 gatcttcatc ggttcagcca tggcttaagt cctttcacag atttccagtt ggccaaagtc
     4321 cgatcagccg catccgccca cggtgatctt ga
//
LOCUS       DBNGWZ010000382         7282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_798856, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000382 DBNGWZ010000000
VERSION     DBNGWZ010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7282
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_798856"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..3034)
                     /gene="uvrA"
                     /locus_tag="AC16CX_11235"
     CDS             complement(113..3034)
                     /gene="uvrA"
                     /locus_tag="AC16CX_11235"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3013119.1"
                     /translation="MQTAGPDAARATEFIRVRGARTHNLKNVNLELPRNRLIVITGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSTYARQFLQLMEKPDVDLIEGLSPAISIEQKAAS
                     HNPRSTVGTVTEIHDYLRLLFARIGDPHCPEHGIQLSAQSIAQMVDHVLELPEDTRLM
                     ILAPLIVGRKGEQADLFEELRAQGFVRLRIDGEVHEIDAIPKLKKTVKHTVEVVVDRL
                     KVRQDAKQRLAESFETALRHADGRVIALQMDEGKPLTPDPSPDKRGEVPDRSRDGKGI
                     EHLFSAKFACPVCSYSLPELEPRLFSFNNPMGACPRCDGLGTISFFDPKRVAAFPDLS
                     LASGAIKGWDRRNQFYFQMLQCLAGHYGFDIETPFGELPEKAREAVLHGSGGEKIRFV
                     YVGDRGNKHVREHAFEGVIPNLERRYRETDSIVVREELAKYLNTRACPECDGTRLRRE
                     ARHVTVAGRTIYEISALTLAHASRFFDVLSLAGSKQAIAEKIVKEILTRITFLNNVGL
                     DYLSLDRAADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQRDNARLLATLKR
                     LRDTGNTVIVVEHDAEAILSADHVVDMGLGAGDHGGNVIAQGTPQDVAKHPESLTGQY
                     LSGRRRIEVPALRHRANPGRQLVISGASGNNLKSISLSVPTGLFVCVTGVSGSGKSTL
                     VNDTLYAAVARALNDSPLEPAAFEAISGVELIDKVVNVDQSPIGRTPRSNPATYTGLF
                     TPIRELFAGVPEARARGYGAGRFSFNVKGGRCEACQGDGVLKVEMHFLPDIYVQCDVC
                     HGERYNRETLDVRYKGKNIHEMLQMTVEQAAEFFSAVPAIARKLQTLLDVGLGYIKLG
                     QSATTLSGGEAQRVKLSLELSKRDTGRTLYILDEPTTGLHFHDIDLLLRVLRRLRDHG
                     NTVVVIEHNLDVIKTADWIIDLGPEGGDGGGRVIAEGAPEDVAKNKASYTGQYLKSVL
                     T"
     gene            3151..4506
                     /locus_tag="AC16CX_11240"
     CDS             3151..4506
                     /locus_tag="AC16CX_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013120.1"
                     /translation="MSRDELRAAASLSSIFGLRMLGMFVILPVFAIYAESLPGGENLT
                     LTGIAIGAYGLTQAILQIPFGWWSDRYGRKPVIYAGLAIFAAGSFIAALGTHIYVIIL
                     GRLLQGAGAIAAPVMAMIADLTRDEHRTKAMAMVGSTIGATFAASLAISPWLNGVIGV
                     PGLFAMTGVLVLIAMGVVYALVPDAPQPHRRSDGRGLDQFMAALRDPQLARLYYGVFA
                     LHAVLMALFIAVPLDLRDSGLPVEAHWKVYLPVMLGSFVLMLPAVLGFTGPERTKRVF
                     VGSVAVLLALHIAMPWFAHGLWAIVVHLLVFFTAFNVLEAALPALVTRIAAPRNKGVA
                     IGVFTSLQFFGAFIGAAAGGFLYSRWGASGVVIFDSALLVIWFIAALGTRVPHPVSTR
                     AYALPALDPIRAERLAAGLGSLPGVHEARVASGEGRVYLVVDSTAFDEQNALKLIPRD
                     S"
     gene            4531..4992
                     /gene="ssb"
                     /locus_tag="AC16CX_11245"
     CDS             4531..4992
                     /gene="ssb"
                     /locus_tag="AC16CX_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3013121.1"
                     /translation="MASVNKVILIGNLGADPETRYLPSGDAVTNIRIATTEKWRDKGG
                     EQQEHTEWHRIAFFGKLAEIAGEYLKKGSPVYVEGRIRTRKWQDKESGQDRYSTEIVA
                     DRMQLLGGRGGGAEPMAREREPASAPAGGNNKPQQQKKGGAFDTMDDDIPF"
     gene            complement(5112..6311)
                     /locus_tag="AC16CX_11250"
     CDS             complement(5112..6311)
                     /locus_tag="AC16CX_11250"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016774 - phosphotransferase activity,
                     carboxyl group as acceptor [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HIR3013122.1"
                     /translation="MSGEILVLNTGSSSIRFALYTAAREPALVLHGMISGIDATPGFG
                     AEDADGKPLPAALPEGGAAPLTHETALARLFDWFADKGYGKELLGAGHRVVHGGVRYA
                     EPIRIDAGTLAELEGLVPLAPLHQPHNLAAIRVLMKLQPGLSQVACFDTAFHRTQPLI
                     AQSFALPREITAGGVLRYGFHGLSYEYIAGILPQYLGDRADGRVVVAHLGAGASMCAM
                     RGRRSVATTMGFTALEGLVMGTRSGAIDPGVVFYLMREKSMTLAEVEDLLYHRSGLLG
                     VSGVSSDMRLLLASNDPHAREAIELFCYRAARELGALAATGSGLDALVFTAGIGEHAA
                     PVRAIICEQARWLGVELDEAANREHRAVISTPSSRVTVCVIPTDEEIVIARHTRRLLR
                     GGARDEG"
     gene            complement(6395..>7282)
                     /locus_tag="AC16CX_11255"
     CDS             complement(6395..>7282)
                     /locus_tag="AC16CX_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HIR3013123.1"
                     /translation="PLLTAVVHPCDPESLRGAVQAVRRNLIRAVLVGPQAKIRRAAEE
                     AGVSIRGLQIITTEHSEAAAEAAVAMAREGRAECLMKGSLHTDELMSAVVAESSGLRT
                     ARRISHAFVLDVPAYPRPLIITDAAINIYPSLETKRDIVQNAIDLAHALGIARPRVAI
                     LSAVETVTPRIHSTIDAAALCKMADRGQITGAILDGPLAFDIAISEAARRTKHVHSAV
                     AGRPDILVVPDLEAGNMLAKQLQYLAGAKSAGVVLGARVPIALTSRADLAESRLASCA
                     IASLLRAHGTKRKSVIGDK"
BASE COUNT         1128 a         2396 c         2470 g         1288 t
ORIGIN      
        1 ggagggcgag agagcgacag aatccgtgag tgcgggaggg cgcgagtgcg cgagggcgaa
       61 aagcaggagt attgaatgcc tttccatttc acgatctccc gatctcacgc cctcacgtca
      121 gtacggactt gaggtactgg cccgtatagc tcgccttgtt cttcgcgacg tcctcgggag
      181 cgccttccgc gatcacgcgc ccgccgccgt caccgccttc gggtccgagg tcgatgatcc
      241 agtcggcggt cttgatgacg tcgagattgt gctcgatcac caccacggtg ttgccgtggt
      301 cgcgcagccg gcgcagcacg cgcagcagca ggtcgatgtc gtggaagtgc agtcccgtcg
      361 tcggctcgtc gaggatgtag agcgtgcgcc cggtgtcgcg cttggaaagc tcgagcgaca
      421 gcttcacccg ctgcgcctcg cccccggaga gcgtcgtggc gctctgcccg agcttgatgt
      481 agccgaggcc cacgtcgagc agcgtctgga gcttgcgcgc gatcgccggc accgcgctga
      541 agaattccgc cgcctgctcc accgtcatct gcagcatttc gtggatgttc ttccccttgt
      601 agcgcacgtc gagcgtctcg cggttatagc gctcaccgtg gcacacgtcg cactgcacgt
      661 agatgtcggg caggaagtgc atctcgacct tgagcacgcc gtcgccctgg cacgcctcgc
      721 agcgcccgcc cttgacgttg aacgagaagc gcccggcgcc gtagccgcgg gcgcgcgcct
      781 cgggcacccc ggcgaagagc tcccggatcg gcgtgaacag cccggtgtag gtggcggggt
      841 tggaacgggg cgtcctcccg atcgggctct ggtccacgtt caccaccttg tcgatgagct
      901 ccacgccgga tatcgcctcg aaggccgcgg gctcgagcgg gctgtcgttg agggcgcgcg
      961 ccaccgccgc ataaagcgtg tcgttgacga gcgtggactt gcccgagccc gagacgccgg
     1021 tcacgcacac gaacaggccc gtcggcacgc tcagactgat gctcttcagg ttgttgccgc
     1081 tcgcgccgct gatgacgagc tggcgcccgg ggttggcccg gtggcgcagc gccggcacct
     1141 cgatccggcg gcgccccgac agatactggc cggtgagcga ctccgggtgc ttcgcgacgt
     1201 cctgcggcgt gccctgcgcg atgacgttgc cgccgtggtc gccggcgcca aggcccatgt
     1261 cgacgacgtg atcggcgctc agtatcgctt ccgcatcgtg ctcgaccacg atgaccgtgt
     1321 tgccggtgtc gcgcaggcgc ttcagggtcg cgagcagccg cgcgttgtcg cgctggtgca
     1381 gcccgatcga gggctcgtcg agcacgtaca tcacgcccgt caggcccgag ccgatctggc
     1441 tcgcgagccg gatgcgctgc gcttcgcccc ccgacagcgt gtccgccgcg cggtcgagcg
     1501 agagatagtc gaggccgacg ttgttgagga aggtgatgcg ggtgaggatc tccttgacga
     1561 tcttctccgc gatggcctgc ttgctgccgg ccagggacag gacgtcgaaa aaccggctcg
     1621 cgtgcgcgag cgtcaacgcg ctgatctcgt agatggtccg gcccgcgacc gtgacgtggc
     1681 gcgcctcgcg ccgcagccgc gtgccgtcgc actcggggca ggcgcgggta ttcaggtact
     1741 tggcgagctc ctcgcgcacc acgatcgagt cggtctcgcg gtagcggcgc tcgaggttgg
     1801 ggatcacgcc ctcgaacgca tgctcgcgca cgtgcttgtt gccgcggtcg ccgacgtaga
     1861 cgaaccggat cttctccccg cccgagccgt gcagcaccgc ttcccgcgcc ttctccggca
     1921 gctcgccgaa cggcgtctcg atgtcgaagc cgtaatggcc cgccagacac tgcagcatct
     1981 ggaaatagaa ctggttgcgc cggtcccagc ccttgatcgc tcccgaggca agcgagaggt
     2041 cggggaaggc ggccacccgc ttcgggtcga agaaactgat ggtgccgagc ccgtcgcagc
     2101 gcgggcaggc gcccatgggg ttgttgaatg agaacagccg cggctcgagc tccggcagcg
     2161 agtagctgca caccgggcag gcgaacttcg cggagaacag gtgctcgatc ccttttccat
     2221 cgcgtgagcg atctggtacc tcccctctct tgtctggaga ggggtcgggg gtgaggggtt
     2281 tgccctcgtc catctggagt gcgatcaccc ttccatcggc gtggcggagc gcggtttcga
     2341 acgattccgc gagacgctgc ttggcgtctt gcctcacctt gagccggtcg acgacgacct
     2401 cgacggtgtg cttgacggtc ttcttcagtt ttggaatcgc gtcgatctca tggacctcgc
     2461 cgtcgatcct cagccgcacg aagccctgcg cgcgcagctc ctcgaagaga tccgcctgct
     2521 cgcccttgcg gccgacgatg agcggcgcga ggatcatgag gcgcgtgtcc tcggggagtt
     2581 ccagcacgtg gtccaccatc tgcgcgatgc tttgcgccga gagctggatc ccatgctcgg
     2641 ggcagtgggg atccccgatg cgcgcgaaca gcagccgcag gtagtcatgg atctcggtga
     2701 cggtgccgac cgtcgagcgc gggttgtggc tcgcggcctt ctgctcgatc gagatcgccg
     2761 gcgacagccc ctcgatcagg tccacgtcgg gtttttccat cagttgcagg aactgccgcg
     2821 cgtaggtgga gagcgactcc acgtagcggc gctgcccctc cgcgtagagc gtgtcgaacg
     2881 cgagcgagga tttccccgac cccgacaggc cggtgatcac gatcaaacgg ttcctcggca
     2941 gctcgaggtt gacgttcttg aggttgtgcg tgcgggcgcc gcgcacgcgg atgaactcgg
     3001 tcgcgcgggc ggcatcgggt cccgcggtct gcagccgtac aacggtcggg cgatgggtgg
     3061 ccaatgtgag gtctggaacg aagctaaacc tgctaatata ccgaagatcc ccgccttttc
     3121 ccaacctgtt tgatgccccc cgcagaccgg atgagccgcg acgaattgcg cgcggcggcg
     3181 agcctttcca gcatcttcgg gctgcgcatg cttggcatgt tcgtgatcct gccggtgttc
     3241 gcgatctatg ccgagagcct gcccggcggc gagaacctca cgctcaccgg catcgccatc
     3301 ggggcctatg gactcacgca ggcgatcctg cagattccgt tcggctggtg gtcggatcgt
     3361 tacggcagga agcccgtgat ttacgccggg ctcgcgatct tcgccgccgg cagcttcatc
     3421 gcggcgctcg gcacccacat ttacgtcatc atcctcggcc gcctgctgca aggcgcgggc
     3481 gcgatcgcgg cgccggtgat ggcgatgatc gccgacctca cgcgggacga acaccgcacc
     3541 aaggcgatgg cgatggtcgg ctcaaccatc ggcgccacct tcgccgcctc cctggcgatc
     3601 tcgccgtggc tcaacggcgt gatcggcgtg ccgggcctct tcgccatgac cggcgtgctg
     3661 gtgctgatcg cgatgggggt ggtgtacgcg ctcgtgcccg atgcgccgca gccgcaccgg
     3721 cgcagcgacg ggcgcggcct ggaccagttc atggcggctt tgcgtgatcc ccagctcgcc
     3781 cgcctctact acggggtgtt cgcgttgcac gctgtattga tggcgctttt catcgccgtg
     3841 ccgctcgacc tgcgggattc aggattgccc gtggaggcgc actggaaggt ctaccttccg
     3901 gtcatgttgg gctcctttgt gctgatgctg cctgccgtac tgggttttac cgggccggag
     3961 cggacgaagc gcgttttcgt cggctcggtt gctgtgctcc tggcgctgca catcgcgatg
     4021 ccgtggttcg cacacgggct gtgggcgatc gtcgtccatt tgctggtgtt tttcacggca
     4081 ttcaacgtgc tggaggccgc gctgccggcg ctggtcaccc ggattgccgc accccggaac
     4141 aagggggtgg cgatcggcgt gttcaccagc ctccagtttt tcggcgcctt cattggcgcc
     4201 gccgccggcg gcttcctcta cagccggtgg ggcgcgagcg gggttgtcat cttcgactcc
     4261 gctctgctcg ttatctggtt tatcgccgcg cttggcaccc gggtgccgca cccggtatcg
     4321 acacgcgcct acgcgctgcc tgcgctcgac ccgatccggg ccgaacggct tgcggccgga
     4381 ttggggtcgc tacccggggt gcacgaggcg cgggtggcga gcggcgaggg gcgggtgtat
     4441 ctcgtggtgg acagcacggc ctttgacgaa caaaatgcgc taaaactgat accacgtgat
     4501 tcgtgaatcg tgattcgtta ggagaagaaa atggcctcag tcaacaaggt gatactcatc
     4561 ggcaatctcg gcgccgaccc ggaaacgcgc tatctgccga gcggggacgc cgtcaccaac
     4621 atccgcatcg ccaccaccga gaaatggcgc gacaagggcg gcgaacagca ggagcacacg
     4681 gaatggcacc gcatcgcgtt cttcggcaag ctcgccgaga tcgcgggcga gtacctcaag
     4741 aagggctcgc cggtctacgt cgagggacgc atccgcaccc gcaagtggca ggacaaggag
     4801 agcgggcagg atcgctattc gaccgagatc gtcgccgacc gcatgcagct cctgggcggg
     4861 cgcggcggcg gcgccgagcc gatggcgcgg gagcgcgagc ccgcttcagc ccccgccggc
     4921 ggcaacaaca agccgcagca gcagaaaaag ggcggggcgt tcgacacgat ggacgacgac
     4981 attccgttct agggatgagg gcggagggcg gagggatgag ggaacaatcc gaagccccgt
     5041 ctcacggttt ggatagccaa tggcgccctt cctcggaagg gcgctttggt tttaaggtca
     5101 tccctcatcc ttcatccctc atccctcgcg ccgccacgca gcaggcggcg cgtgtggcgc
     5161 gcgatgacga tctcctcgtc ggtcggaatg acgcagaccg tgacgcggct tgacggcgtg
     5221 ctgatgacgg cgcggtgctc gcggttggca gcctcatcga gctcgacgcc gagccagcgc
     5281 gcttgctcgc agattatcgc gcgcaccggc gccgcgtgct caccgatgcc tgcggtgaag
     5341 accagggcgt cgagcccgct tcccgtcgcg gcgagcgcac ccagctcgcg cgcggcccga
     5401 tagcagaaca gctcgatcgc ctcacgggcg tgggggtcgt tgctggcgag gaggagccgc
     5461 atgtcactgc tcacgcccga tacgccaagc aggcccgagc ggtggtagag cagatcctcc
     5521 acttccgcga gcgtcattga tttttcccgc atcaggtaga acaccacgcc gggatcgatc
     5581 gcgccgctgc gcgtgcccat taccaggcct tccagagcgg tgaagcccat ggtggtcgca
     5641 acgctccggc gcccgcgcat cgcgcacatg cttgccccgg cgccgaggtg cgcgacgacg
     5701 accctgccgt ccgccctgtc gccgaggtat tgcggcagga tgcccgcgat gtactcatac
     5761 gacagcccgt ggaagccgta gcgcaatacg cctcccgccg tgatctcccg cggcagcgcg
     5821 aagctttgtg cgatcagcgg ctgggtgcgg tggaaggctg tatcgaagca ggcaacttgg
     5881 gagaggcccg gctggagctt catcaacacg cggatggcgg cgaggttgtg cggctggtgc
     5941 agcggcgcga gcggcaccag cccctcgagc tcggcgagcg tgccggcgtc gatacggatg
     6001 ggctcggcgt agcgcacgcc gccgtgcact acgcggtggc cggcgccgag cagctccttg
     6061 ccatatcctt tgtccgcaaa ccagtcgaac agccgtgcga gcgccgtctc gtgggtgagc
     6121 ggggccgcgc caccttcggg cagcgccgcc ggcagcggct tgccgtccgc atcctcggcg
     6181 ccgaagccgg gcgtggcatc gatcccggag atcatgccgt gcaatacgag cgccggttcg
     6241 cgcgccgcgg tgtagagcgc gaacctgatg ctcgacgacc ccgtattcag gacgagtatt
     6301 tcgccgctca tttttgtaaa gaggcgtgat aagtgacaag tgataagtga taagagaccc
     6361 gcgctccgcg cggcaccagc ttacctatca cccatcactt atcacctatc acgctttttc
     6421 gttttgtgcc gtgggcgcgc aggagcgagg cgatcgcgca tgacgcaaga cgcgattccg
     6481 ccaggtcagc gcggctggta agcgcgatcg gcacccgcgc gccgagcacg acgccggctg
     6541 atttcgcgcc ggcgaggtac tggagctgct tcgccagcat gtttccggcc tccagatcgg
     6601 gcacgacgag gatgtcggga cggccggcca ccgcggagtg cacgtgcttg gtgcgccgcg
     6661 ccgcctcgga gatcgcgatg tcaaaagcga gcggcccgtc cagtatcgcg cccgtgatct
     6721 ggccgcggtc cgccatcttg cacagcgccg cggcatcgat ggtggaatgg atccgcggcg
     6781 tcaccgtttc cacggcggac aatatcgcga cccgcgggcg ggcaatcccg agcgcgtggg
     6841 cgagatcaat ggcgttttgc acgatgtcgc gcttcgtttc gaggctggga tagatgttga
     6901 tcgccgcgtc ggtgatgatc agcgggcgcg gataagccgg cacatcgagc acgaaggcat
     6961 ggctgatgcg ccgcgcggtg cgcaacccgc tgctttcggc caccacggcg gacatcagct
     7021 cgtcggtgtg caggctaccc ttcatcaggc actccgcgcg tccctcgcgg gccattgcga
     7081 ccgcagcctc tgcggccgct tcgctgtgtt cagtcgtgat gatctggaga ccgcggatgc
     7141 tcacccccgc ttcctccgcc gcgcgccgga tcttcgcctg cgggcccacg agcacggccc
     7201 ggatcaggtt cctgcgcacc gcctggaccg caccgcgcag ggactcggga tcgcagggat
     7261 gcacgaccgc ggtgagcagg gg
//
LOCUS       DBNGWZ010000383         7693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3318907, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000383 DBNGWZ010000000
VERSION     DBNGWZ010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3318907"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="AC16CX_11260"
     CDS             <1..1148
                     /locus_tag="AC16CX_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3013124.1"
                     /translation="NVIKVEPLDGEHLRSRGDLGGAALPFAMLNSNKKPVTLNLKTEK
                     GRELFREMVARADILVENFAPGVMDRLRIGPADLHKINPRLIYGSSSGYGKSGPYRDY
                     PAMDLVMQAMSGVINSTGFPDQPPVKSGAAICDFMAGIHLYAAIVTALYERERTGKGR
                     VVEVSMQDATYASLASNLGMVHARGAAAPERTGNRHGGLGISPYNVYPTKDGYVVLNA
                     PGDHHFRAILDVMGRPDLKEDPCFLTRSARVVNFAAVDELIKGWTKTLTKNEVAQRML
                     AAKVPCAPVRNLMEVMHDENMHARGSLQWIDHPELGRVVLLHSPLVFEGTERRPIEPS
                     LPLGASNDAIFGEWLGHSKGELAAYKSEGVIGRSQAELPAHEAEGFI"
     gene            1224..2576
                     /locus_tag="AC16CX_11265"
     CDS             1224..2576
                     /locus_tag="AC16CX_11265"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013125.1"
                     /translation="MTQSLTLTLAQYAASTSIKAIPGNVRERAKQVILDEMASACFGR
                     RRTAGELAARYATSFGGPTECRILGTRLRAPAPYAALANGTAGHADEIDGAHVVGGHP
                     GAAIVHAALAVAERQRASGAELLNAVVLGYDVGIRLVEACGSKLPFKKRTHLYSDFLY
                     AGGAAAAGCRLLGLDPVRHCHAMALVTFQTNSLASLYQERRHISKSLCNGQFSFAGVS
                     AALMSATGLEGCDDILGAKDGVLDAWGVEHGAELVTGGLGQDYAVMGANFKFLNAGYP
                     IHAAVEAAMTLVADHKIGADAIASVHVGMPANAMRVVDNREMHNICVQDMVSVALVRG
                     GLRLRESYFPAILSDPAFGRMRSRITVGVDRGLEADQPNGRGANVTITTVHGSTVSQR
                     VDHPRGHSRRGAVAWSDLSEKWHDALPGCDVDRMLALAQRLDDLEDVNDLSDAFGTKS
                     "
     gene            2910..3101
                     /locus_tag="AC16CX_11270"
     CDS             2910..3101
                     /locus_tag="AC16CX_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013126.1"
                     /translation="MLDKVRVMEGKETTLAGPLFAAEFLPVARYSPPRLGEHTQEVLQ
                     SLGYSDAVIRALRDKGAVG"
     gene            complement(3254..4054)
                     /locus_tag="AC16CX_11275"
     CDS             complement(3254..4054)
                     /locus_tag="AC16CX_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3013127.1"
                     /translation="MNRDSKSSQLVTVRLEVREAGHVAYISVNNPEKRNALGIAGKKQ
                     LAGAFRNLAKDKTLRVAVLTGAGDRSFIAGADIAEMKDLTPKGAKDEHTWTHRACEAI
                     RTLPVPVIARINGYCFGAGMELAASCDVRIGVTTAKFGMPEVRFGIPSGMEACLLPQL
                     IGWGKTRELVYTGDHIDAAEAHRCGFLEKRVEPSGLDSSVERWVSSILAAGPRAIRLQ
                     KKLVRDWERMTIEQAVRAGIRACVEARRTDEPRRLMAAFLERKKGGKA"
     gene            complement(4102..4578)
                     /locus_tag="AC16CX_11280"
     CDS             complement(4102..4578)
                     /locus_tag="AC16CX_11280"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3013128.1"
                     /translation="MTAAKSIRIGLISDTHGLARDEALAALEGCRHIIHAGDIDTPDV
                     LAKLRRLAPVTAVRGNNDRGSWAQAIRAFEVVEFGEVSVYVRHDQSELDIDLMTAGFR
                     VIVFGHSHRPVVESRDGVLFVNPGSAGPRRFRLPVAVGELLISGDRVDARIIELVA"
     gene            complement(4599..5336)
                     /locus_tag="AC16CX_11285"
     CDS             complement(4599..5336)
                     /locus_tag="AC16CX_11285"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3013129.1"
                     /translation="MTVVLLSLATFFSTLAGGLFALRFRDRLHFLLSFTAGVLLGVVS
                     LDILPEIFELAHEHGFDASDAMIALVVGFLLFHGLEKFVLVHHIHEADYAQHHHPRVG
                     VLSALALIGHSFMDGVGIGLAFQVAPAVGVAVAIAVIAHDFCDGLNTVGLMLVHHNTP
                     LRSAAMLALDALAPVLGAASTLVVQVPPHLLMLYLGFFAGFLLYIGASDVLPEAHSQA
                     NPAIALSLIGLTCLGAAFIYMVVRLAG"
     gene            complement(5542..6141)
                     /locus_tag="AC16CX_11290"
     CDS             complement(5542..6141)
                     /locus_tag="AC16CX_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3013130.1"
                     /translation="MKNKLWYAVLGAVLMSAGSCLAQQKPPEVRRIVTGLDASGKAIV
                     MFDSAVQLTSFRSPNPAGEMWVTPKSPPDFSWTEDRAKIKVPLTPPPGGTIFRIVDFV
                     PLTPEAEKKMDINMMMRVVGDHAPRKGLPPKHPMMHRTRSLDYAIILSGEIDMMLDDA
                     VVHLKAGDVVVQQATNHAWVNRGKEVCRIAFILMDSQEP"
     gene            complement(6204..>7693)
                     /locus_tag="AC16CX_11295"
     CDS             complement(6204..>7693)
                     /locus_tag="AC16CX_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR3013131.1"
                     /translation="AGTLTKYKMFIGGEWIDSASGETFPSDNPYTGKPWALIPKGGAR
                     DADRAVRAAHKAFTSGEWPKLNASKRGTLLRKLGDLTAEKSKFLAEVEVRDNGKLFAE
                     MSAQTAYMAQWYYYYGGLADKIEGHVIPTDKPEMFNYTRYEPLGVVVAITPWNSPLLL
                     TAWKLAPALAAGNTVVLKPSEFTSASVLEFMKLVEQAGFPPGVVNVVTGFGPDVGTPL
                     VEHPLVAKVAFTGSDRTGTLIYQTAAKGLKRVSMELGGKSPNIVFDDAHLENAIKGVV
                     SGIFAATGQTCIAGSRLLVQQSIHDEFVEKLVAFAKTAKMGNPMNLDTQVGPVTNPPQ
                     FKKILDYIDIARGEGAKPVLGGGKASRPECGDGWFVEPTIFTGVRNSMRIAQEEVFGP
                     VLAVIPFKDEEEAVAIGNDVVYGLAAGVWTQNMRRALMMAERLQAGTVWVNTYRAVSY
                     LSPFGGYKRSGLGRESGQEMIKDYMQVKSVWISTAMEVPNPFILR"
BASE COUNT         1424 a         2428 c         2467 g         1374 t
ORIGIN      
        1 cgaacgtgat caaggtcgag ccgctcgatg gcgagcatct gcgcagccgc ggcgatctcg
       61 gaggggctgc ccttccgttc gcgatgctca actccaacaa gaagccggtc acgctcaatc
      121 tcaagacaga aaaaggccgc gaactcttcc gggagatggt ggcccgcgcc gacattctcg
      181 ttgagaactt cgctccgggc gtcatggacc gcctcaggat cggccctgcg gatctccaca
      241 agatcaatcc gcgactgatc tatgggtcga gctcgggtta cgggaagtcc ggtccgtatc
      301 gcgactaccc tgccatggat ctggtcatgc aggcgatgag cggcgtcatc aattcgaccg
      361 gctttccgga ccagccgccg gtcaagtccg gcgcggcgat atgtgacttc atggccggca
      421 ttcatctcta tgccgcgatc gtgaccgcgc tttacgagcg cgaacgcacc ggcaaaggcc
      481 gcgtcgtcga agtctccatg caagacgcga cctatgcatc gcttgcctcg aatctgggca
      541 tggtgcatgc gcgcggcgcc gccgcgccgg aacgcaccgg caaccggcat ggcggcctcg
      601 gcatctcgcc ctacaacgtc tatccgacga aggacggcta tgtggtgctc aacgcgccgg
      661 gcgaccatca tttccgcgcg atcctcgacg tcatgggccg gcccgatctc aaggaggatc
      721 cgtgcttcct tacccgctcc gcgcgagtcg tcaatttcgc cgccgtggac gagctaatca
      781 aggggtggac gaagacgctt accaagaacg aggtggcgca acgcatgctt gcggcgaagg
      841 tgccctgcgc gccggtgcgc aatttgatgg aggtcatgca cgacgagaac atgcatgcgc
      901 gcggcagctt gcaatggatc gaccatcccg agcttggccg cgtcgtcctg ctccattcac
      961 ccctcgtctt cgagggaacg gagcgccggc cgatcgagcc cagcctgccg ctgggcgcga
     1021 gcaacgacgc catcttcggc gaatggctcg gccattcgaa gggggagctg gcggcttaca
     1081 agtcggaagg cgtgatcggc cgctcgcaag cggagttgcc ggctcacgag gcggaaggct
     1141 ttatctgagg gcgcgcaagc ccgtcagctc cgaggcagat agcgggcacc ggcggcgaca
     1201 acacaggaag acgcaggcaa gtcatgaccc agtcccttac gctgacactc gcgcaatacg
     1261 cagcctccac ctcgatcaag gcgattcccg gcaacgtcag ggagcgtgcc aagcaggtga
     1321 tcttggacga gatggcgagc gcctgctttg gcaggcgtcg cacagctggc gaactggcgg
     1381 ctcgctacgc cacgagcttc ggcggtccga cggagtgccg gatactgggg actcggctgc
     1441 gggcgcctgc gccgtatgca gcgctggcga acggcacggc gggccatgct gacgagatcg
     1501 acggcgcgca cgtcgtcgga gggcaccccg gtgcggctat cgtccacgct gcgctagcag
     1561 tggctgaacg acagcgcgcg tcgggcgccg aactgctcaa cgcggtcgtg ctcggatacg
     1621 acgtgggtat ccggctggtc gaggcctgtg gcagcaagct cccgttcaag aagcgcaccc
     1681 acctgtattc cgacttcctg tatgccggcg gcgcggcggc ggcgggctgc cgcctgctgg
     1741 ggctggatcc ggtccggcac tgccatgcga tggccctggt caccttccag accaacagcc
     1801 tggcgtcgct gtaccaggag aggcggcaca tcagcaagtc gctgtgcaac ggccagttct
     1861 ccttcgctgg cgtcagcgcg gcgctgatgt ctgccaccgg gctcgagggc tgtgacgaca
     1921 tcctgggcgc gaaggatggc gtgctcgatg cgtggggcgt cgagcacggc gcggagctcg
     1981 tgaccggagg actcggtcag gactacgcgg tcatgggcgc gaacttcaag ttcctgaacg
     2041 cgggttatcc gattcatgcc gcggtcgagg cggccatgac gctggtcgcc gatcacaaga
     2101 tcggcgccga cgcgatcgcg tcggtccacg tcggcatgcc agcaaacgcg atgcgggtcg
     2161 tcgacaaccg agagatgcac aacatctgcg tccaggacat ggtcagcgtg gctctagtgc
     2221 gtggtggcct gaggctgagg gaatcgtact tcccggcaat cctcagcgat ccggctttcg
     2281 gtcgcatgcg gtcgcgcata acggtcggcg tcgaccgcgg gctcgaagcc gatcagccta
     2341 acgggcgcgg agcgaatgtc actatcacca ccgtacacgg gtcaaccgtg tcgcagcgcg
     2401 tcgaccaccc acgaggccac agccgccgcg gcgcggtcgc atggtccgat ctctcggaga
     2461 agtggcacga cgcgctgccg ggatgcgacg tcgacaggat gcttgcactc gctcaacggc
     2521 tcgacgacct tgaggacgtc aacgatctgt ccgacgcgtt cggcacgaag agttgatgtg
     2581 ccaacgatgg cagcccgtgc cggatggctc caccggtctt gcgaccacct gtggccgcag
     2641 gcgatgcggc atacgtattg gcccggcagc ggcgtcgcgg gcgattccgg attcgatcag
     2701 ttctatgcgt cgcaggtgcc gcgcgtgacg gatatgaccg cgcttggagc catgatgtac
     2761 gaggctggtg tggccgggcg ccagcaacga cgccgtcatg agcgaataat tctgccattc
     2821 ggagcaggcg ttggcggctt acaaggcaag aggcgtcaac taaaggagcg ccagcccgtc
     2881 gaccccgagg ttatccagag ccgacctgcg tgctggacaa ggtgcgggtc atggaaggga
     2941 aggagacgac gctggcggga cccctgttcg cggcggaatt cctgcctgta gcccgttact
     3001 cgccgccacg cctcggggag cacacgcagg aagtgctgca gtccctcggt tacagcgacg
     3061 cggtaatccg ggcgctgcgc gacaagggcg ccgtcggcta ggtgcccccc gcgcacgccg
     3121 cgaggagagt cttggcgtcg tgctctagca gatacatttt tcaaaggcgt gataggtgac
     3181 gagtgatagg tgataggaaa gcttcaagac cacaaccgac gttcaaccct taccacttat
     3241 cacctatcac ttatcacgct tttccgcctt tcttgcgttc aagaaaggcg gccatgagcc
     3301 gcctcggctc atcagtgcgg cgcgcttcga cgcacgcacg tatgcccgcc cgtaccgcct
     3361 gctcgatggt catccgctcc cagtcgcgca ccagcttctt ctggagccgg atcgcgcgcg
     3421 ggccggccgc gaggatggaa gagacccacc gctcgacgct ggagtcgagc cccgagggct
     3481 cgacccgctt ttccagaaaa ccgcaccgat gcgcctcggc agcatcgatg tgatcgccgg
     3541 tatagaccag ctcgcgcgtc ttgccccagc cgatcaattg cggcagcagg caggcttcca
     3601 tgcccgaagg aatgccgaag cgcacttccg gcatgccgaa tttggcggtg gtgacgccga
     3661 tacgcacgtc gcacgaggcg gcaagttcca tccccgcgcc gaagcaatag ccgttgatgc
     3721 gcgcgatcac tggaacaggc aaggtgcgga tcgcttcgca cgcgcggtgg gtccaggtgt
     3781 gctcgtcttt tgctcccttc ggcgtcaggt ccttcatttc ggcgatatcg gcgccggcaa
     3841 tgaaggagcg gtctcccgcg ccggtgagga cggcaacacg cagcgtcttg tccttggcga
     3901 gattgcgaaa ggctccggcg agctgcttct tgccggcaat gccgagcgcg ttgcgttttt
     3961 ctgggttgtt gaccgagata taagcaacgt gcccagcctc ccgcacctcg aggcgcacgg
     4021 tcaccagctg actggatttc gaatcacgat tcacgggttt tcgcggcgac ccacgaccta
     4081 caatccacga tctacgttct ttcaagcaac cagctcgatg atccgtgcgt caacccggtc
     4141 gcccgagatc agcagctcgc cgaccgcgac cggcagcctg aagcggcgcg gtccggcgct
     4201 gccaggattg acgaataaca cgccgtcccg ggattccacg acgggcctgt gcgaatgtcc
     4261 gaacactatg acccggaatc cagctgtcat caggtcgatg tccagctccg actggtcatg
     4321 ccggacgtag accgacacct cgccgaactc gaccacttcg aaagcgcgga tcgcttgcgc
     4381 ccacgatccc ctgtcgttat taccgcgcac tgccgtgaca ggcgcaagcc tgcgcagctt
     4441 tgccaggacg tccggcgtgt cgatatcgcc ggcatgaatg atgtgccggc atccttcgag
     4501 cgccgcgagc gcctcatccc gcgcgaggcc atgcgtgtcg gaaatgaggc cgatgcggat
     4561 ggatttcgct gcggtcatcc cagggctcga ttccggcgct atccggcgag gcgaacgacc
     4621 atgtagatga acgcggcccc caggcaggta agccctatga ggctgagcgc gatggccggg
     4681 ttggcctgag agtgcgcttc gggcaggacg tcggaagctc caatatacag gaggaacccg
     4741 gcgaaaaatc ccagatagag catcaagagg tgagggggaa cttgaacgac cagggtcgat
     4801 gccgcgccca gaacaggagc gagcgcatcc agcgcaagca tcgccgcgga acgcagaggc
     4861 gtgttgtggt gaaccagcat caggccgacc gtgttgaggc catcgcaaaa gtcatgcgcg
     4921 atcacggcta tggccacagc gaccccgacg gctggagcga cctggaacgc cagaccgatg
     4981 ccgacgccgt ccatgaagct gtgtccaatc aacgcaaggg cggagaggac gcccactcgc
     5041 ggatggtgat gctgggcgta atcggcctcg tggatgtgat ggaccagcac gaatttctcg
     5101 aggccatgaa atagaagaaa cccgaccacc agcgcaatca tcgcgtcact ggcatcgaac
     5161 ccgtgctcgt gggccagctc gaagatctcg ggaaggatgt caagactgac gacacccagc
     5221 aggacaccgg cagtgaagct cagaaggaag tgcagccggt cgcggaacct gagcgcaaac
     5281 agccccccgg ccagggtcga aaaaaacgtt gcgagagaca gcaatacgac ggtcattgca
     5341 ttatacgcag tggtcagcca cggcgcttga agtactggat cgcgcgttca tgcttcacgg
     5401 ccttggcacg agtcctgaac gttccaaaga ccagcgcaag gggtcaggcg tgcatagttg
     5461 catatttgaa ctcttgaccc actctcccgg tgttgcaatt ggccaggcct gccctccttc
     5521 tcccggattt cgttcatgcg gtcagggttc ctgcgaatcc atcaggatga aggcgatgcg
     5581 gcagacctcc ttcccacggt tgacccaggc gtggttggtc gcctgctgga ccacgacgtc
     5641 gcccgccttg aggtgcacga cggcgtcgtc gagcatcatg tcgatttcgc cggacagaat
     5701 gatggcgtaa tcgagactgc gcgtgcggtg catcatcgga tgcttgggcg gaaggccttt
     5761 cctgggtgcg tggtcgccga ccaccctcat catcatgttg atgtccattt ttttctcggc
     5821 ctcgggcgtg aggggcacga aatccacgat ccggaagatc gtgccgcccg gcggcggcgt
     5881 aaggggaacc ttgatcttcg cgcggtcctc ggtccaggaa aagtcgggcg ggctcttggg
     5941 ggtgacccac atttcaccgg cggggttggg cgagcggaag ctcgtcaact gcaccgcgct
     6001 gtcgaacatc acgatcgcct tgccggaggc atcaagcccg gtcacgatgc ggcggacctc
     6061 gggcggcttc tgctgcgcaa ggcaagaacc cgcggacatc agcacggctc ccagaaccgc
     6121 ataccagagc ttgtttttca tgggctactc cctgagttgg ttgatcaaaa agccgacgcg
     6181 cgctggatgg aacctgccgc tcgtcaccgt agtatgaacg gattcggcac ttccatcgcc
     6241 gtcgagatcc acacgctctt cacctgcatg tagtccttga tcatctcctg cccgctctcg
     6301 cggccaagtc cgctgcgctt gtatccgccg aagggagaga ggtaggacac cgcgcggtag
     6361 gtgttgaccc acaccgtgcc ggcctggagg cgttccgcca tcatgagcgc gcggcgcatg
     6421 ttctgcgtcc agacgccggc ggcaaggccg taaaccacgt cgttgccgat cgcgaccgct
     6481 tcctcttcgt ccttgaacgg gatcaccgct agaaccgggc cgaacacctc ctcctgcgcg
     6541 atgcgcatgg aattcctgac cccggtgaaa atcgtgggct cgacgaacca gccgtcgccg
     6601 cattcggggc ggcttgcttt ccctcctccc agcacaggct ttgcgccctc gcctctggca
     6661 atgtcgatgt agtcgaggat cttcttgaac tggggcggat tggtgaccgg tcccacctgg
     6721 gtgtcgagat tcatcgggtt gcccatcttc gcggtcttgg cgaaggccac gagcttctcg
     6781 acgaactcat cgtgaatgct ttgctgtaca agcagtcgcg agcctgcgat gcaggtctgc
     6841 cccgtcgcgg cgaagatgcc cgagaccacg cccttgatcg cgttctccag gtgcgcgtcg
     6901 tcgaacacga tgttgggcga cttgccgccg agctccatgc tgacgcgctt caagcccttg
     6961 gcggcggtct ggtagataag agtgccggtc cggtccgagc cggtgaacgc caccttggcg
     7021 accagcgggt gctcgaccag cggcgtgccg acgtcgggcc cgaagccggt gacgacattg
     7081 actacgccag gcgggaaacc ggcttgctcg accagcttca tgaactccag caccgaggcc
     7141 gaggtgaact cggaaggctt cagcaccacc gtattgccgg ccgcgagcgc gggcgcgagc
     7201 ttccacgcgg tgagcagcag gggagaattc cagggcgtaa tggcgactac gacgcccagg
     7261 ggctcgtagc gcgtgtagtt gaacatctcc ggcttgtcgg tgggaatgac gtgcccttcg
     7321 atcttgtctg cgagcccgcc gtagtagtaa taccactgcg ccatataggc ggtctgcgcg
     7381 ctcatctcgg cgaagagctt gccgttgtcg cgcacttcga cctcggcgag gaatttcgac
     7441 ttctcggcgg tcaggtcacc gagcttgcgc aacagcgtgc cgcgcttcga ggcgttgagc
     7501 ttcggccact cgccggaggt gaacgccttg tgcgccgcgc gcaccgcgcg gtccgcgtcc
     7561 cgcgcgccgc ccttcgggat cagcgcccac ggcttgccgg tgtacgggtt gtcgctcggg
     7621 aaggtttcgc cggaggcgga atcgatccat tcgccgccga tgaacatctt gtatttcgtg
     7681 agagtgccgg ctg
//
LOCUS       DBNGWZ010000384         2336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3319001, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000384 DBNGWZ010000000
VERSION     DBNGWZ010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3319001"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC16CX_11300"
     CDS             <1..193
                     /locus_tag="AC16CX_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013132.1"
                     /translation="VQSHWHASSTETLMAKLGIDWQAKKAEFVGYLEEVKAGKQENLY
                     DPRKMITSGPGYIPIAKRP"
     gene            190..1245
                     /locus_tag="AC16CX_11305"
     CDS             190..1245
                     /locus_tag="AC16CX_11305"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3013133.1"
                     /translation="MTQPILIVGAGPAGLAAAHALATVGQKPILVEKEQRLGGAPILS
                     GYAKLVPSGEWAKDAIGGMVSRVESNSLVTVHRGCTVKEFGGEPGAFRAVLSNGAAVD
                     AGAAILCTGFTHFDSVNKPEWGFGTFPDVVTTTQIEQMISSGKGVRCPSDGRKPERVA
                     ILLCVGSRDRQIGREWCSKICCTVSCNLAMEIREELPDAHVYIYYMDIRTFGLYETNY
                     YWRSQEEFKVKYIKARIAEVTSDGKRLIIKGEDTLVKRPITIPFDLVVHAIGMDPNVD
                     NKSLSRTFGFELERHGFIAKGNSYGALGETSRPGVFVAGAATSPETIDDSIAQAKAAA
                     LSAYALVSRAVEPAAAA"
     gene            1257..2117
                     /locus_tag="AC16CX_11310"
     CDS             1257..2117
                     /locus_tag="AC16CX_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013134.1"
                     /translation="MVRPAASHASSAAGLERPQLELNGPKLALAFETLVSGTEEHGGV
                     ERYVEALKLKSELFRRALGAGKARSLELATFKALATLIAPVRRRAAAYLVNPSFEQAR
                     ARLACLLDGAEDTTTTDRRIAEFCAGFPADRQHRWVRDLAADVLHNVDPERYPLMCRW
                     VWDAGTNTGAIRELWHAGDADHIRIDVPDRYGTFVMLREELAQFLAANGVFRDVIQYV
                     DLLTAQVYAGYISEQGGSYLRTDFSAPADPVEHTRRLLGLDGVDARGRTRLKSFDGEA
                     FVVDDVKLLD"
     gene            2125..>2336
                     /locus_tag="AC16CX_11315"
     CDS             2125..>2336
                     /locus_tag="AC16CX_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013135.1"
                     /translation="MPIHEQSLIRPENIQSHDKLVIDGVDVSGHWSTFIASRVVTDYD
                     EKLQDEIAALPGGEYIHRCWQCGSCT"
BASE COUNT          397 a          800 c          786 g          353 t
ORIGIN      
        1 cgtgcagtcg cactggcacg cctcctccac cgaaacgctg atggcgaagc tcggcatcga
       61 ctggcaggcc aagaaggccg agttcgtggg ctacctcgag gaggtcaaag ccggcaagca
      121 ggagaacctc tacgacccac gcaagatgat cacctccggc ccgggctaca ttccgatcgc
      181 gaaacggccg tgactcaacc gattctgatc gtcggcgcgg ggccggccgg gcttgccgcc
      241 gcgcatgcgc tggccaccgt cggccagaag cccatcctgg tggagaaaga acaacgcctg
      301 ggcggagcgc cgatcctctc gggttacgcg aagctggtgc cctccggcga gtgggcgaag
      361 gacgcgatcg gcggcatggt gagccgcgtc gagtccaatt ccctggtgac ggtccatcgc
      421 ggctgcacgg tgaaggagtt cggcggcgag cccggggcgt tccgtgccgt gctttccaat
      481 ggggccgccg tggacgccgg cgcggccatc ctttgcaccg gcttcaccca cttcgactcg
      541 gtcaacaagc cggaatgggg cttcggcacg tttccggacg tggtgacgac gacccagatc
      601 gagcagatga tctcctcggg caagggcgtg cgttgcccct ccgacggccg caagcccgag
      661 cgcgtggcga tcctgctctg cgtcggctcg cgcgaccggc agatcgggcg cgagtggtgc
      721 tcgaagatct gctgcacggt gtcctgcaac ctcgcgatgg agatccgcga agagctgccg
      781 gacgcccacg tctacatcta ctacatggac atccgcacct tcggcctgta cgagacgaac
      841 tactactggc gcagccagga ggagttcaag gtcaagtaca tcaaggcgcg catcgcggag
      901 gtcacctccg acggcaagcg gctgatcatc aagggcgagg acaccctggt caagcgcccg
      961 atcacgatcc cgttcgacct ggtggtgcac gcgatcggca tggaccccaa cgtcgacaac
     1021 aaatcgctca gcaggacctt cggcttcgag ctcgagcgcc acggcttcat cgccaaggga
     1081 aattcatacg gcgcgctggg ggagacctcc cgtccgggcg tcttcgtcgc cggtgccgcg
     1141 accagtccgg aaacgatcga cgactccatc gcccaggcaa aggccgctgc cctatccgcc
     1201 tacgcgctgg tgagccgcgc ggtggaaccc gcggcggcgg catgaccgga gcgtgtatgg
     1261 ttcgcccggc ggcatcgcac gcgtcgagcg cggcggggct ggagcggccg cagcttgagc
     1321 tgaacgggcc caagctcgcg ctggcgttcg agacgctggt ctcgggcacc gaggagcacg
     1381 gcggggtcga gcgctacgtc gaggcgctca agctcaagtc ggagctgttc cggcgcgcac
     1441 tcggcgcggg caaggcgcgc agcctcgagc tcgcgacatt caaggcgctc gccaccctga
     1501 tcgcgccggt gcgccggcgc gcagccgcct atctcgtcaa cccctcgttc gagcaggcgc
     1561 gcgcgcgcct cgcctgcctg ctcgacggcg ccgaggacac cacgaccacc gaccggcgca
     1621 tcgccgaatt ctgcgccgga tttcccgcgg accggcagca tcgctgggtg cgcgatctcg
     1681 cggccgacgt cctgcacaac gtcgacccgg agcgctatcc cctgatgtgc cgctgggtgt
     1741 gggacgcggg gaccaatacc ggcgcgatcc gcgagctgtg gcacgccggg gatgccgatc
     1801 acatccggat cgacgtgccg gaccgctacg gcaccttcgt catgctgcgc gaggagctcg
     1861 cccagttcct cgccgcgaac ggcgtgttcc gcgacgtgat ccagtacgtc gatctgctga
     1921 ccgcgcaggt ctacgccggg tacatctccg agcagggcgg cagctacctg cgcaccgatt
     1981 tcagcgcgcc cgccgatccc gtcgagcaca cgcggcggct gctcgggctg gacggcgtgg
     2041 acgcgagggg gcgcacgcgg ttgaagtcct tcgacggcga ggccttcgtg gtggatgacg
     2101 tgaagctgct cgactagccc ggccatgccg atccacgaac agtcgctgat ccgtcccgag
     2161 aacatccagt cgcacgacaa gctcgtgatc gacggggtgg acgtctccgg ccactggagc
     2221 acgttcatcg cctcgcgcgt cgtcaccgac tacgacgaga agctgcagga cgagatcgcg
     2281 gcgctgccgg gcggcgagta catccaccgc tgctggcaat gcggctcgtg caccaa
//
LOCUS       DBNGWZ010000385         4529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_421449, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000385 DBNGWZ010000000
VERSION     DBNGWZ010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_421449"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1059)
                     /locus_tag="AC16CX_11320"
     CDS             complement(1..1059)
                     /locus_tag="AC16CX_11320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3013136.1"
                     /translation="MVNIPVPRCSRWPAGLRAMSYLAFAGVAVGVCTPLAAQDKPPSA
                     PPAAQTQPELTVDALSVVKVRSKAVANARTLRSLGTQREGTGVVIDSDGLVLTIGYLI
                     IEAETVELSTADGRSFPATVVGYDSVTGFGLLKALRPLPVKPVQFGHSAHIGEREMVL
                     IVGFDGVAPAYVVSRRPFVGYWEYLLEEAIYTAPATVNWSGAALLSREGKLLGIGSLV
                     VGDAMGTRTQVPGNMFVPIDLLKPMLGDLIATGKSTARPRPWIGINTQEVQGNVIVSR
                     VSPESPADDANIRTGDVIVGVGGQPIKGQADFYTRLWGRGEAGTSIPLEVLRGGRVET
                     ITVKSIDRDRYYRPKPTY"
     gene            1242..2054
                     /locus_tag="AC16CX_11325"
     CDS             1242..2054
                     /locus_tag="AC16CX_11325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013137.1"
                     /translation="MIYNPAFFLQGSIVELGLKGKVAAVTGGTEGIGRATALRLAQEG
                     ASVAICARRQEPLDKMAAEIRKTGAGVLAISADMSKAADVERFMKAVIERFGRIDILV
                     NNAGTSARGKFLEVDDGTWNADLDLKVFGAIRCARLAVPHMRKQGGGRIINITISSAK
                     QPGAESMPTSVSRAAGLAITKALSKEFAPDNIRVNTVCIGKIKSGQHERRYTKQGISA
                     DEYYRQVSTDIPLGRVGEAEEVANVIAFLVSDAASYVTGTSINLDGGISGTL"
     gene            2295..3236
                     /locus_tag="AC16CX_11330"
     CDS             2295..3236
                     /locus_tag="AC16CX_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013138.1"
                     /translation="MLFGLMGLESRAQEYPAKPIRLIVPFPPGGGNDTIARLMGQKLA
                     PVLGQQILVDNRPGAGGTIGAELAAKSPPDGYTLFLAGVASHGINPNLRKKLPYDPLR
                     DFHGVSLIASAPLLVVVHPSLPVTSVKQLVALAKGKPGLINFASNGTGGSSHLAGELF
                     MMMTGTAMVHIPYKGLSPALTDLLSGQVQLMFSSAVAMLPQVKAGKLRAIAMTGAKRS
                     QAIPDIPTVAEAGVPGYETGSWYGIVAPAKTPRPVIERLSREIAAVTRSPEISGRLVD
                     EAVIPIGSSPDEFDAHIKRELARWAKVIAKARISEEQ"
     gene            complement(3358..3433)
                     /locus_tag="AC16CX_11335"
     tRNA            complement(3358..3433)
                     /locus_tag="AC16CX_11335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:Thr,seq:cgt)
     gene            complement(3630..4046)
                     /locus_tag="AC16CX_11340"
     CDS             complement(3630..4046)
                     /locus_tag="AC16CX_11340"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3013139.1"
                     /translation="MRLMNYTRDNLAEVLRRHDINPTHQRIEIAHALFSRGEHLSADQ
                     VLAIVNGRHSETSKATVYNTLNLFLEKRLIREVIVDPNKVFYDPNTEPHYHFYNVDTG
                     ELTDIDAAGVEVHGLPALPEGMVTEGVDVIVRIRSR"
     gene            4359..>4529
                     /locus_tag="AC16CX_11345"
     CDS             4359..>4529
                     /locus_tag="AC16CX_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3013140.1"
                     /translation="MTRNNNSLPAAIGKHGGFTLIELMIVVAIIAILAMIAFPSYDRY
                     IIRSNRAVAKQFM"
BASE COUNT          852 a         1458 c         1426 g          793 t
ORIGIN      
        1 tcagtaggtg ggcttgggcc ggtaatagcg gtcgcggtcg atggacttca ccgtgatggt
       61 ctcgacgcga ccgccgcgca ggacctcgag gggaatgctg gtgcccgctt cgccgcgccc
      121 ccacagcctg gtgtagaagt cggcctgccc cttgatcggc tgaccgccga cgccgacgat
      181 cacgtcgccc gtgcggatgt tggcgtcgtc cgcggggctt tccggcgaga cccggctgac
      241 gatcacgttg ccctgcactt cctgggtgtt gatgccgatc cacgggcgcg ggcgcgccgt
      301 ggacttgccg gtggcgatga ggtcgcccag catgggcttc aacaagtcga tcggcacgaa
      361 catgtttccg ggaacctgcg tgcgcgtgcc catcgcgtcg cccaccacca gcgagccgat
      421 gccgagaagc ttgccttcgc ggctgagcag cgccgcccca ctccagttga ctgtggcggg
      481 agcggtgtag atcgcctcct cgaggagata ttcccagtag ccgacgaagg gccggcgcga
      541 caccacgtag gccggcgcaa cgccatcgaa gccgacgatc agcaccatct cgcgctcgcc
      601 gatatgggcg gaatgcccga actgcaccgg cttgacgggc agcgggcgca acgccttgag
      661 caggccgaag ccggtgacgc tgtcatagcc gacgaccgtg gccgggaacg agcgtccatc
      721 ggcggtggac agttccaccg tctcggcctc gatgatcaga taaccgatgg tgagcaccag
      781 gccgtcggag tcgatcacca cgccggtgcc ctcgcgctgc gtgcccagcg agcgcagggt
      841 gcgggcattg gcgaccgcct tgctccgcac cttgacgacc gagagcgcgt cgaccgtgag
      901 ctccggctgg gtttgcgccg caggtggggc cgatggcggc ttgtcctgcg cggcaagggg
      961 agtacatacc ccgaccgcca cccctgcaaa cgcgagatag ctcatcgcgc gcaaaccggc
     1021 tggccagcgg ctgcagcggg ggactggaat gttcaccatg taatcgttct cctgataaca
     1081 gcatatccta cgaccattcg gcgcgtttgg taatgggcgt acggcacacg gatcgtcata
     1141 agcagtcctg caagcgtgtc acagttcaca tcgacgcgac atcccggggc aggcgccagc
     1201 acagcctgcc ggtgcccgcg catcgatacg gccgcgcagg catgatttac aatccggctt
     1261 tcttccttca ggggtccatc gtggagctgg gattgaaagg caaggtcgcc gcggtcacgg
     1321 gcggcaccga aggcatcgga cgcgcaacgg cgttgaggct cgcgcaggag ggcgcaagcg
     1381 tggcgatctg cgcgcggcgg caggaaccgc tcgacaagat ggcggctgaa atcaggaaga
     1441 ccggcgccgg cgtgctcgcc atttccgccg acatgagcaa ggccgccgac gtcgagcgct
     1501 tcatgaaagc cgtgatcgag cgcttcggcc gcatcgacat cctcgtcaac aacgccggca
     1561 cttcggcgcg cggcaagttc ctcgaggttg acgacggaac gtggaatgca gacctcgatc
     1621 tcaaggtgtt cggcgcgatc cgctgcgcgc ggctcgccgt gccgcacatg agaaaacagg
     1681 gcggcggccg catcatcaac atcacgatct cgagcgccaa gcagccaggc gcggaatcca
     1741 tgccgacctc ggtttcgcgc gccgcgggtc tcgccatcac caaggcgctg tcgaaggaat
     1801 tcgcgcccga caacatccgc gtcaacacgg tttgcatcgg caagatcaag tcgggacagc
     1861 acgaacggcg ctacaccaag cagggcatca gcgccgacga gtattaccgg caggtgtcca
     1921 cggacatccc gctcgggcgc gtcggcgagg cggaggaggt cgcgaacgtc atcgcctttc
     1981 tcgtctcgga cgcggcgagc tacgtcaccg ggaccagcat caatctcgac ggaggcatct
     2041 cggggaccct atgaaaagcc ggatcgggcc aggagtttgc cggatgaggc gacaaactcc
     2101 cgaggcaaaa ccgcctgctg aaaaacagcg aagagggtca ttgatatgcg aattcgaccc
     2161 ccagtctcct cgccaatgac cgggcctaag ggcttgttcc attacatccc atgttaaatt
     2221 tccagacggt tttgctcata gcagcctgtc ggagcgctaa gcccgacagg ctgctatttg
     2281 cgctggcggc gtgcgtgctc ttcgggctca tggggctcga atcgcgcgcc caggaatatc
     2341 cggcgaaacc gatccggctc atcgttccgt tcccgcccgg cggcggcaac gacaccatcg
     2401 cgcggctcat gggccagaag ctcgcgccgg tgctcggcca gcagatcctg gtggacaacc
     2461 ggccgggcgc gggcggcacg atcggagccg agctggcggc caagtcgcca cccgacggct
     2521 atacgctgtt cctcgccggc gtggcaagcc atggcatcaa tcccaacctg cgcaagaagc
     2581 tgccctacga tcccctgcgc gacttccacg gcgtgagcct gatcgcctcc gcgccgctgc
     2641 tggtggtggt gcacccctcg ctccccgtga cgtccgtcaa gcagctcgtc gcgctcgcga
     2701 aaggcaagcc gggcttgatc aatttcgcct ccaacggcac cggcggctcg tcccacctcg
     2761 ctggagagtt gttcatgatg atgaccggca ctgcgatggt ccacatcccc tacaaggggc
     2821 tgtcgccagc gctgaccgac ctcctgtccg gccaggtgca gctcatgttc tcgagcgcgg
     2881 tggcgatgct gccgcaggtc aaggccggga agctgcgcgc gatcgccatg accggcgcga
     2941 agcgctcgca ggccatcccc gatattccga ccgtggccga ggcgggtgtg cccggctacg
     3001 agaccgggtc gtggtacggc atcgtcgcgc ccgcgaagac gccacggccc gtcatcgagc
     3061 ggctgtcgcg cgagatcgcc gccgtcaccc ggtccccgga gatctccggc cgcctggtgg
     3121 atgaggccgt gatacccatc ggcagctcgc cggacgaatt cgatgctcat atcaagcgcg
     3181 agctcgcgcg ctgggccaag gtcattgcaa aggccaggat cagcgaggag cagtaagcga
     3241 cgatccgcga tgagcgaccg cgcagtccgg cgtggtgtaa ccggcaagac cgcagcggct
     3301 gcctgatttc ctgttaccgg gtcggtataa aaaaggttgc cggacggcaa ccttttttgg
     3361 tgctggcgag tggactcgaa ccaccgacct actgattacg aatcagttgc tctaccagct
     3421 gagctacgcc agcttaggaa tttgccggaa accaacgcgg caaattcctt ataaaaatta
     3481 cctgcgagat ctgattatcc tcgattcccg ccatcgctgg aatatcggga ccacaatcaa
     3541 ttggtgtttt ctcaagcccg cacgcccagc cgtcaccacg cgaaatttcc ttgtcttgct
     3601 cagaccgtgg cagtcgcgat acgtgcgggc tagcgcgaac ggatgcgtac gatgacgtcc
     3661 acgccctcgg tcaccatccc ctcgggcagc gccggcaggc cgtgaacctc gacgccggcg
     3721 gcgtcgatgt cggtcagctc accggtgtcc acgttgtaga aatggtaatg cggctcggtg
     3781 ttgggatcgt agaacacctt gttggggtcc acgatcacct cccggatcag cctcttctcc
     3841 aggaacaggt tgagcgtgtt ataaaccgtc gccttggagg tctcggagtg gcggccattg
     3901 acgatcgcca gcacctggtc ggccgacagg tgctcgccgc gcgagaacag agcgtgcgcg
     3961 atctcgatgc gctggtgcgt cggattgatg tcgtggcgcc gcagcacctc ggccaggttg
     4021 tcgcgcgtgt aattcatcag cctcatgaag ctcgataccg gattatgagc caagatctgg
     4081 actgagtcta attcggcccc cggctcgctt ccgtgacgga ttgccgcacg ggcgcgagaa
     4141 atcgtgcaca tcctcgaaat caggctgtcc tatcggccgc cggatgcccg ctcccggcca
     4201 gcgcgtagcg cattgttccg aatgctaaaa tcaccacgcg acgagtgagg cagcggatgc
     4261 ggaaaccgtt cgttcaccaa ccgggcccac ttatcggctg gctttgccgg agcgcttcat
     4321 ccctgattaa tatttgaacc gcctgaaagg acaacgacat gacacgcaat aataattcgt
     4381 tgccggctgc gatcgggaaa cacgggggat tcacgctgat cgagctgatg atcgtggtgg
     4441 cgatcatcgc aatattggcg atgatcgcct tcccgtcgta cgaccgatat atcatccgct
     4501 cgaatcgcgc cgtcgccaag caattcatg
//
LOCUS       DBNGWZ010000386         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_672853, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000386 DBNGWZ010000000
VERSION     DBNGWZ010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_672853"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..815
                     /locus_tag="AC16CX_11350"
     CDS             30..815
                     /locus_tag="AC16CX_11350"
                     /EC_number="4.1.3.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HIR3013141.1"
                     /translation="MSKRKTLRTDIAWSTTDRITVKGFDLCKDILGGMSLGDMAFLEL
                     TDRLPTPKESVVFNAIAVTLVEHGLTPSAIATRMTYAGAPEAMQAAVAAGLCGLGSVF
                     VGSMETAARMLQEALPDPAKKADLEQLAHEIVGRFHSRKQAVPGIGHHFHKPVDPRAP
                     RLFEIATENGFDGPYIKLMKRVAQEAEAVYGKSLPVNATGAIAAIACELKLPWRIVRG
                     IGVMARAIGLVGHIREEMREPIAREIKTRAEEEATAHLRSRDS"
     gene            877..1098
                     /locus_tag="AC16CX_11355"
     CDS             877..1098
                     /locus_tag="AC16CX_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013142.1"
                     /translation="MSDAELKRWAETWERAGRELARIRREELQRMTDEQARAAALDLL
                     SMPIPEGLPERESGLVEQQRLFSKLRTPR"
     gene            complement(1326..>2489)
                     /locus_tag="AC16CX_11360"
     CDS             complement(1326..>2489)
                     /locus_tag="AC16CX_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3013143.1"
                     /translation="VAAPFATRHLADLGARVIKIERPKVGDFARDYDRTVMGMSSHFV
                     WLNRSKESLTLDVKHPGAKEILMRLIARADVFVQNLAPDATTRLGLAAETLLEMHPRL
                     IVCDISGYGDSGPYASKKAYDLLVQSEAGVLSVTGTPEIPSKVGISIADIATGVYAFS
                     GVLTALLQREKTGKGGRVEVTMLEALGEWMGYPLYYAHFSGAAPPRTGPDHATIVPYG
                     RYRTGDGKSVMLGLQNEREWVVFCDKVLGQPALATDSRYDSNFKRNEHRAEITALIEK
                     VFLTLTTEQVLEKLDAAGIANARLNTPEEVWQHAQLKARERWRDVGSPIGEIPALLPP
                     ATISGVEARMDPIPEIGEHTESILAELGFSGREINELNAAGAISFERRKPPIK"
BASE COUNT          459 a          784 c          806 g          440 t
ORIGIN      
        1 cgactgagat aaccgccagg gagcatcaca tgagcaagag gaaaacactg cgcaccgaca
       61 tcgcctggag cacgaccgac cggatcacgg tcaagggctt cgacctgtgc aaagacattc
      121 ttggcggaat gtcgctcggc gacatggcgt tcctcgaact gaccgatcgg ctgccgacgc
      181 cgaaggagtc ggtcgtgttc aacgcgatcg cggtgacact ggtcgagcac ggtttgacgc
      241 cgagcgccat cgcgacccgc atgacctacg ccggcgcgcc ggaagcgatg caggcggcgg
      301 tcgcggccgg cctgtgcggg cttggcagcg tattcgtggg cagcatggaa acagctgcgc
      361 ggatgctgca ggaggcgctg cccgatccag cgaagaaagc ggatctcgag cagcttgcgc
      421 atgaaatcgt cgggcgcttc cacagccgca agcaagccgt tcccggcatc ggccatcatt
      481 ttcacaagcc ggtggacccg cgcgcgccgc ggctattcga gatcgccacg gaaaacggct
      541 tcgacgggcc ttacatcaag ctgatgaagc gcgtggcaca ggaggcggaa gcggtttacg
      601 ggaaatccct gccggtcaac gccaccggcg ccatcgccgc gatcgcctgc gagttgaagc
      661 tgccatggcg gatcgtccgc ggcatcggcg tcatggcacg cgccatcggg ctggtgggcc
      721 acatccgcga agaaatgcgc gagccaatcg cccgcgagat caagacccgt gcggaggagg
      781 aggccaccgc ccaccttagg agccgtgatt cgtgattcgt gggtcgtggg tcgtgggtcg
      841 tggttcgcaa gcaaatagca gaggtaaaac agggttgtga gcgacgctga actcaagcga
      901 tgggccgaga cctgggagcg cgccggacgc gagctggcgc gcattcggcg cgaggaactc
      961 cagcgcatga cggatgagca ggctcgagcg gccgccttgg acctgctcag catgcccata
     1021 cccgaagggc tgccagagcg cgaatccggc ctcgtagagc agcaacgcct attctcgaaa
     1081 ctgcgcactc cccgatgaaa accgtcctcg gggcagccct cgaggtgcag tgtttcctgt
     1141 cccgcagtgg cgaattggaa gcaggtgttc gacgagattg aaccgctcgc ggcggtgcgt
     1201 gaggtgcccg gattgctgga acggctgcgg caacttcgga gcagcgcttc tgagcagggc
     1261 tgagtgagtg cgtacgccgc gtgatgggga ggcgtgagtc gactcacgac ccacgatcca
     1321 cgctcttact tgatcggcgg tttgcgccgt tcgaatgaaa tcgcgcctgc ggcgttcagt
     1381 tcgttgattt ccctaccgct gaagccgagc tctgccagta tgctctcggt gtgctctccg
     1441 atctccggaa tcgggtccat gcgcgcctcc acgccggaaa tggtcgccgg cggcagcaag
     1501 gccgggattt cccctatggg cgaccctacg tcgcgccacc gttcacgcgc cttgagctgg
     1561 gcatgctgcc agacttcttc gggggtgttc aagcgcgcgt tggcgatgcc cgcagcgtcc
     1621 agcttctcga gcacctgctc cgtcgtcagg gtaaggaaca ccttttcgat gagcgcggtg
     1681 atttcggcgc ggtgctcatt gcgcttgaaa ttgctgtcgt agcgcgaatc ggtcgccagc
     1741 gcgggctggc caagcacctt atcgcagaac accacccact cccgctcgtt ctgaagcccg
     1801 agcatcacgc tctttccgtc cccggtccga tagcggccgt acggcacgat ggttgcatga
     1861 tccggacccg tgcgcggcgg cgcggccccg ctgaaatgag cgtaataaag agggtagccc
     1921 atccattctc ccagcgcctc gagcatcgtc acttccacgc gcccgccttt tccggttttc
     1981 tcccgctgga gaagcgcggt cagtactccg gaaaaggcat acacgccggt cgcgatgtcg
     2041 gcgatcgaga tgccgacctt gctcggtatt tccggcgttc cggtcaccga aagcaccccc
     2101 gcctcgctct gcaccagaag gtcataggct ttcttcgatg cgtacggccc ggagtccccg
     2161 tagcccgaaa tatcgcagac gataagccgc ggatgcattt ccagcaacgt ttccgccgcg
     2221 agcccaagac gtgtcgtggc gtccggcgcg aggttctgga cgaacacgtc ggcgcgcgcg
     2281 atgagccgca taagtatttc cttcgcgccg ggatgcttga cgtcgagcgt gaggctttcc
     2341 ttggagcggt tcagccagac gaaatgggag gacatcccca tgaccgtgcg gtcgtaatcg
     2401 cgcgcgaaat cgcccacctt cgggcgctcg atcttgatca cgcgcgcgcc gagatcggcg
     2461 aggtggcgcg tggcgaacgg cgccgccac
//
LOCUS       DBNGWZ010000387         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2564139, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000387 DBNGWZ010000000
VERSION     DBNGWZ010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2564139"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="AC16CX_11365"
     CDS             <1..1164
                     /locus_tag="AC16CX_11365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3013144.1"
                     /translation="EFARRHEAARKLMARDGFDALVLTGGPDIYSHGSGVTWGAGLID
                     ERGMCQYMVLPLKGEPTLIYPHYGCHIEAARKMVSVRDVRSGQHGKYGKAVAERLIEL
                     GLQKGRIGITAANRTGPEYMGVQAYQDMLKLLPQATFVFCPALLHELTYRKSAEELRA
                     MARAGQLAIKALEAVKAAAKPGVREYQLAGAVANTVLSAGGRHHLLMIGSTSMHDPKL
                     IFPNPNPSARVLKEGDIILSELAMSYMGYSAKIGHPVSIGRPTERYQAFFKDVVLGGF
                     KEIAALLKPGTTLEDVRKVGAQAFRKRGAQSRPTIMHGLDLITSVPFVRADRVRSEPY
                     EATMQPGMTYNIEITPVDKEGVFGIFYSRSFAITETGKQDLTPYDVDEILVAG"
     gene            1355..2221
                     /locus_tag="AC16CX_11370"
     CDS             1355..2221
                     /locus_tag="AC16CX_11370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013145.1"
                     /translation="MPNAPGSSADTMGRIAAARLGEALGQQIVVDNRAGAGGVVGLEI
                     GKNSNPDGYTLITTSLGALTVSPHIRKNLPYDPIRDFEYVALYSRQGNVLVVNPALPV
                     KSVRDLIDYAKAKQGKLNMATAGIGAQSHLNGTALMMAGKFESVAVPYKGGGPSMAAV
                     IANEAQWSIAPAGAMMSHVRAGRLRAIGHTLPQRSPLMGDIPAIAETLPGFKYIAFSG
                     FLAPKGTPRAVIQKINATMAKVVSAPEMKEQLAFQGAEPATGTPEVFRKAIADEIVET
                     GKLVKAIGLKAE"
BASE COUNT          427 a          718 c          792 g          359 t
ORIGIN      
        1 gaattcgcgc gccggcacga ggcggcgcgc aagctcatgg cccgggacgg cttcgacgcc
       61 ctcgtcctga ccggagggcc ggacatctac agccacggca gcggcgtgac ctggggcgcg
      121 gggctgatcg acgagcgcgg catgtgccag tacatggtgc tgccgctcaa aggtgagccg
      181 acgctgatct atccccatta cggctgccat atcgaggccg cgaggaaaat ggtatcggtg
      241 cgcgacgtgc gcagcggcca gcacgggaag tacggcaagg ccgtcgccga gcggctgatc
      301 gagctcggcc tgcagaaagg ccgcatcggc atcaccgcgg cgaaccgcac cgggcccgag
      361 tacatgggag tccaggccta ccaggacatg ctgaagctgc tgccgcaggc cacgttcgtt
      421 ttctgcccgg cgctgctgca cgagctgacc taccgcaaga gcgcggagga gctgcgcgcg
      481 atggccaggg ccggccagct cgcgatcaag gcgctcgagg cggtcaaggc ggccgcgaag
      541 ccgggcgtgc gcgagtacca gcttgcgggc gcggtggcga atactgtgct gagcgcgggc
      601 ggccgccacc acctgctgat gatcggctcg acctcgatgc acgatcccaa gctcatcttt
      661 ccgaatccca atccgtcggc gcgcgtgctg aaggagggcg acatcatcct gtcggagctt
      721 gcgatgtcct acatgggcta ctcggcgaag atcgggcacc cggtcagcat cggcaggccg
      781 accgagaggt accaggcctt cttcaaggac gtggtattgg gcggcttcaa ggagatcgcg
      841 gcgctgctca agcccggcac cacgctcgag gacgtgcgca aggtcggcgc ccaggccttc
      901 cgcaagcgcg gggctcaatc acggcccacc atcatgcacg ggctcgatct catcacgtcg
      961 gtgccgttcg tccgtgccga ccgggtgcgc agcgagccgt acgaagcgac catgcagccg
     1021 ggcatgacct acaacatcga gatcacgccg gtggacaagg agggcgtgtt cggcattttt
     1081 tactcgcgct cgttcgcgat caccgagacc ggcaagcagg acctcacgcc gtacgacgtg
     1141 gacgagattc tggtcgccgg atgacatcgg tgacgagtga cgagtgtcga gtgacgagtg
     1201 atgaggtgat gaagtgaagt cgaaagtcga aagtcgaaag tcgaaaggga aggtgttagg
     1261 ccgatctttc ttttgccttt cgactttcgc cttttgcctt ggtattgctg gggcgagcgc
     1321 ccagcaatac ccggttaggc cgatccgtct cgtcatgccc aacgcgccgg ggagctcggc
     1381 ggacaccatg ggccgcatcg ccgccgcgcg cctgggcgag gcgctcggcc agcagatcgt
     1441 ggtggacaac cgcgcgggcg cgggcggcgt cgtgggtttg gaaatcggca agaactcgaa
     1501 tccggacggc tatacgctca tcacgacctc gctcggcgcg ctgacggtgt cgccccacat
     1561 ccgcaagaac ctgccgtacg acccgatccg ggatttcgag tatgtcgcgc tctactcgcg
     1621 gcagggcaac gtgctggtcg tcaacccggc gctgccggtg aagagcgtca gggatctcat
     1681 cgattacgcg aaggcgaagc agggcaagct caacatggcc accgcgggca tcggcgcgca
     1741 gagccatctc aacgggaccg ccctgatgat ggcgggcaaa ttcgagtccg tagcggtgcc
     1801 gtacaagggc ggcggtccct cgatggccgc cgtgatcgcg aacgaagcgc agtggtcgat
     1861 cgcgccggcc ggcgcgatga tgtcccatgt cagggccggg cggctgcgcg cgatcgggca
     1921 caccctgccg caacgctcgc ccctgatggg tgacatcccg gcgatcgccg aaaccctgcc
     1981 ggggttcaag tacatcgcgt tttccggatt cctcgcgccg aagggaacgc ccagggccgt
     2041 gatacagaag atcaacgcga ccatggcgaa ggtcgtcagc gcgccggaga tgaaggagca
     2101 gctcgcgttc cagggcgccg agcccgcgac cggcactccc gaggtgttcc gcaaggcgat
     2161 agccgacgag atcgtggaaa ccggcaagct ggtgaaggcc atcggactca aggctgagtg
     2221 accccctccc tcaccctccc ccttggcaag ggggagggat agggtggggg tagagtgtcg
     2281 agtgtcgagt gacgag
//
LOCUS       DBNGWZ010000388         4934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3068482, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000388 DBNGWZ010000000
VERSION     DBNGWZ010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3068482"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16CX_11375"
     CDS             complement(<1..407)
                     /locus_tag="AC16CX_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013146.1"
                     /translation="MAVVHNQGIEIFFEDSGAGLPVVLGHSFLCSGKMWREQVPALCA
                     KFRVINPDLRGHGRSGHARRPFSLYDALSDVIAVLDRLGIERATWCGLSIGGMVALRA
                     ALTHPDRVAGLILLDTDAGPETGLRKLKFRAMGA"
     gene            complement(800..1366)
                     /locus_tag="AC16CX_11380"
     CDS             complement(800..1366)
                     /locus_tag="AC16CX_11380"
                     /inference="COORDINATES: protein motif:HMM:NF025595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HIR3013147.1"
                     /translation="MSAHGSHVDPSNKRVALLIAILAAALAFSETLGKSAQTAGLGYH
                     IEVSNLWSFFQAKTIRMTTLRTSAEALETDLAREQNARAKEAMEKRIAEWRKTAARYE
                     SEPDTQEGRKELATRAKETEKKRDRSLAAYHQYEMSSAALQLAIVLATANIITGVAFL
                     LWGSMGLGVVGVAFILIGFFAPTSVHLF"
     gene            1507..1941
                     /locus_tag="AC16CX_11385"
     CDS             1507..1941
                     /locus_tag="AC16CX_11385"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3013148.1"
                     /translation="MKFEGFLHLNIRCSESDLPAIEKFYGDVLGLKKGYRPNFKFGGL
                     WLYDGDDPIIHVGARFPKGSIVKDKHSGSVDHIAFKASGAADFRKRLKKLGIEFEEQN
                     IENAGYQVFLYDPVGTKLEFNFLNEHVTDAVPLGTTAEANLR"
     gene            complement(2103..2756)
                     /locus_tag="AC16CX_11390"
     CDS             complement(2103..2756)
                     /locus_tag="AC16CX_11390"
                     /inference="COORDINATES: protein motif:HMM:NF008228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HIR3013149.1"
                     /translation="MFEAFVHKIFWPFFLGGLVGLMSISNPLSKIPLFVSLTEDMAER
                     ERRTIARKACLYGFIMLTFTLFFGVFILEAFGISYGALRIAGGLTIAALGYRMLFGYG
                     YVGLTSKGGQRNIAVFPLALPAISGPGAIAVVIGISTEIAELRTAVNQAVAYSAAVTS
                     ILLTCIAIWLTFRSARFISRVTGPDAMEATTRLMGFLLVCIGVQFVASGVRTFIAGQ"
     gene            complement(2887..3519)
                     /locus_tag="AC16CX_11395"
     CDS             complement(2887..3519)
                     /locus_tag="AC16CX_11395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3013150.1"
                     /translation="MLESTAPTRLEKPAPVNRPIHDHFVRRWSSRAFDLKRPVPRELL
                     MTLLEAGRWAPSCNGDEPWRYLIWDRNRDPEGFQKAFDCLSDNNKKWVKNVPLLMLSC
                     AGSIFQATGKPNRWTQHDTGAASVCMALQAVALGLMVHQMGGFDAEKARAAFNIPAEY
                     TPMAMIAVGYQAGAETIDDEDVKKKELAPRARKPVAERFFEGGWGKGVAA"
     gene            complement(3756..4553)
                     /locus_tag="AC16CX_11400"
     CDS             complement(3756..4553)
                     /locus_tag="AC16CX_11400"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3013151.1"
                     /translation="MTGLLAQYGLALVFVNVFLTQVGVPVPSIPTLIVAGALAQQGRL
                     SAALVLGTAVAASLLGDVPWFVGGRVFGYRVLNTLCRVAIEPDSCVKQTENIFERWGA
                     PSLVFAKFIPGFSIVAPPIAGALRLATLPFFVYSAIGAAVWAGVAIAAGMIFHAQVDW
                     LIKWLAEMGSRAMLIIGFAVALYVAVKWVERWLLIRWLRMARISVDELHELLKRDGDT
                     VILDARSATARKLDPRRIPGAVAVDIGALPAALAAVPPDRDVVVYCT"
     gene            complement(4699..>4934)
                     /locus_tag="AC16CX_11405"
     CDS             complement(4699..>4934)
                     /locus_tag="AC16CX_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HIR3013152.1"
                     /translation="WTVEFNEWLADAVKSRRMDDLVAYRERAPHAVRNHPTDEHLIPL
                     FVALGAGGPDAAARRLHTSIESGVISMDAYRFD"
BASE COUNT          948 a         1595 c         1500 g          891 t
ORIGIN      
        1 gcccccatgg cgcggaactt gagcttgcgg agtccggtct ccggaccggc gtccgtgtcc
       61 aggagaatca gcccggcaac tcggtcgggg tgagtcagtg ccgcgcggag agccaccatg
      121 ccgccgatgg acaggccgca ccaagtcgcc cgctcgatgc caagccgatc gagtacggcg
      181 atgacgtctg acagggcgtc gtacagcgag aaaggacgtc tcgcgtgacc ggaccgtccg
      241 tgcccacgga ggtcgggatt gattactcgg aacttggcgc aaagtgccgg tacttgctcg
      301 cgccacatct tgccggagca cagaaaacta tggccgagga caacaggtag ccccgcaccg
      361 ctgtcctcga agaagatctc aatgccttgg ttgtggacga ccgccactgg tatccccgaa
      421 gcgtgagagc gggtgagccg ggcgccccgc catcttcaaa tggccgtcgc ctaactcgta
      481 ttagttgccc gagggagaga cgggggtcaa gtctcgcatt gacgcaaaat atactatttt
      541 gcaagacctg actccgtttt cgcgttcgtc accccggcat acgccggggg tccagtgaaa
      601 tcaaatcctt tctggatccc ggctttctcc gggatgactc atgctgagct tccttaaccc
      661 tttctctttg cttttcttgg cggtgttggt gtccccggat cgagtccggg gcaagtttag
      721 cggttcaata attcggtgat tcgcaggtcg cggatcgtgt ttttcgaatc ctgtaaatcc
      781 tgttgatcct gtctattttc tagaacaagt gcaccgacgt cggggcaaag aacccgatca
      841 ggatgaacgc caccccaacc acgcccagcc ccatcgagcc ccacagcagg aacgcgacgc
      901 cggtgatgat gttagcggtg gcgagcacga tcgcaagctg cagcgccgcc gatgacatct
      961 catactggtg ataggccgcg agcgagcggt cgcgcttctt ctcggtctcc ttcgcgcgcg
     1021 tggcgagctc cttgcggccc tcctgcgtgt ctggctcgga ctcgtaacgc gcggcggtct
     1081 tgcgccattc ggcgatgcgc ttctccatcg cctccttcgc ccgcgcgttc tgctcgcgcg
     1141 cgagatcggt ctcgagcgcc tccgccgagg tgcgcagcgt cgtcatgcgg atcgtcttcg
     1201 cctggaagaa cgaccaaagg ttagagacct cgatgtgata gcccaggccc gcggtctgcg
     1261 cgctcttccc cagcgtctcc gaaaacgcga gcgccgcggc cagtatggcg atcagcagcg
     1321 cgaccctctt gttggacgga tccacgtggc taccgtgtgc tgacatgttc tcactccttt
     1381 gtattgaatg aatgtgtatt ttgccccgac tgctcctggt aaaaaagcgt cgtgcgggag
     1441 accggccgcg gcgctttttt tgtgctagga tttcggccag caaattctgg cgccaaggag
     1501 ggcatcatga aattcgaggg cttcctgcat ctcaacatcc gctgctccga gagcgacctg
     1561 cccgcgatcg agaaattcta tggtgacgtg ctcggcctga agaaaggcta ccggcctaac
     1621 ttcaagttcg gcggcctctg gctctacgac ggcgacgacc ccatcatcca cgtcggggca
     1681 cgcttcccga aaggctcgat cgtcaaggac aagcacagcg gcagcgtgga ccacatcgcg
     1741 ttcaaggcct cgggcgcggc ggacttccgc aagcgcctga agaagctcgg catcgagttc
     1801 gaggagcaga acatcgagaa cgcgggctat caggtcttcc tctacgaccc ggtcggcacc
     1861 aagctcgagt tcaacttcct caacgagcac gttaccgacg ccgttccgct cggcaccacc
     1921 gccgaggcca acttgcgtta gcggcgcgcg tctggaagca aaaaagcccg cctcacggcg
     1981 ggcttttttt atcacgcgcg tcgctaactt agaaaggcgt gataggtgat aggagccggc
     2041 ttccgcgcga agcgcgggta ccttatcact tatcactcgc cacttatcac tcattgccgg
     2101 gctcactggc cggcaatgaa agtccgcacg ccggaggcga cgaactgcac cccgatgcac
     2161 accagcagga agcccatcag gcgggtcgtc gcttccatcg catcgggtcc cgtgactctc
     2221 gagatgaagc gcgccgagcg gaacgtgagc cagattgcga tgcaggtgag gaggatgctc
     2281 gtcacggccg cgctataggc gaccgcctgg ttgaccgccg tcctcagctc ggcgatctcg
     2341 gtcgagatgc cgatcaccac cgcgatcgcc cccggcccgc tgatcgccgg cagcgcgagc
     2401 gggaataccg cgatgttgcg ctggccgccc ttcgacgtga gaccgacgta gccgtacccg
     2461 aacagcatgc ggtagccgag tgccgcgatg gtgaggccgc ccgcaatgcg cagcgcgccg
     2521 tacgagatgc cgaacgcctc caggatgaac accccgaaga acagcgtgaa ggtcagcatg
     2581 atgaaaccgt agaggcaggc cttgcgtgca atggtgcgcc gctcgcgctc cgccatgtcc
     2641 tcggtcagcg agacgaacag ggggatcttc gacaggggat tgctgatgga catcagtccc
     2701 accagcccgc cgaggaagaa cggccagaag atcttgtgga cgaaagcctc gaacatgaag
     2761 gcggaaggcg aaagtcgaag ggcggaagga aatgccaggc ttgttgcttc gcgtcgcctt
     2821 ccccttcgga ccttggcctt tgctttaaac ccgctacttt cgactttcgc cttccgactt
     2881 tcgccttcag gcggcgacgc ccttgcccca gccgccttcg aaaaagcgct cggcgacggg
     2941 cttgcgcgcg cgcggcgcga gttccttctt cttgacgtct tcgtcgtcta tcgtttcggc
     3001 cccggcctga tagccgaccg cgatcatcgc catcggggtg tactcggccg ggatgttgaa
     3061 agcagcgcgc gccttctcgg cgtcaaagcc gcccatctgg tgcaccatca atccgagcgc
     3121 gaccgcctgc aatgccatgc agacgctcgc cgcgccggta tcgtgctgcg tccagcgatt
     3181 gggctttccc gtcgcctgga aaatgctgcc cgcgcaggag agcatcagca gcggcacgtt
     3241 cttgacccat ttcttgttgt tgtcggacag gcaatcgaac gccttctgga agccctccgg
     3301 gtcgcggttg cggtcccaga tgagatagcg ccacggctca tcgccgttgc aggagggcgc
     3361 ccagcgcccc gcttcgagca gcgtcatcag cagctcgcgc ggaaccggcc gcttgaggtc
     3421 gaaggcgcgc gagctccagc ggcgcacgaa atgatcgtgg atggggcggt tgaccggtgc
     3481 gggtttttca agtctggtcg gtgcggtgct ttcaagcata taggcaactc cattctcatg
     3541 tttgaaacaa aggttgatga cgcctcaagg ctaccacgat tgcgggccgg ctatcagcca
     3601 tcagcgtccg gcctgggtca ctcggtcgtt tcaaaaactt cggtctgccc gcctgcctcg
     3661 agccaggctt caaggccgcc cgcgagcggg cgcaccttgc ggaacccctt ctccatcagc
     3721 aggcgggcga cacgcgccgc gctcgcttcg ttcggtcacg tgcagtaaac gaccacgtcg
     3781 cggtcgggcg gcaccgcggc cagcgccgcc ggcaacgcgc cgatatccac ggcgacggcg
     3841 ccgggaatgc ggcggggatc gagcttacgc gcggtcgcgg agcgcgcgtc cagtatcacc
     3901 gtatcgccgt cgcgcttcag gagctcgtgc aactcgtcaa ccgagatccg cgccatgcgc
     3961 agccagcgta taagcagcca ccgctcgacc catttgaccg cgacatacag agcgacggcg
     4021 aagccgatga tgagcatcgc gcgcgagccc atctcggcaa gccacttgat cagccagtcc
     4081 acctgggcgt ggaagatcat tcccgccgca atcgcaacgc ccgcccagac agccgccccg
     4141 atcgcgctgt aaacgaaaaa cggcaaggtc gcgaggcgca acgcgcccgc gatgggcgga
     4201 gccacgatgg aaaaaccggg aatgaacttc gcgaatacga gcgaaggcgc gccccagcgt
     4261 tcgaaaatgt tctcggtctg cttgacgcag gaatcaggtt cgatcgccac gcggcacagc
     4321 gtattgagca cgcggtagcc gaacacccgg ccccccacga accacggcac atccccgagg
     4381 agcgacgcgg caaccgccgt gcccagcacc agcgcggccg acagtcgccc ctgttgcgcc
     4441 agcgccccgg cgacgatcag cgtcgggatg gacggcaccg gcacgccgac ctgggtcagg
     4501 aacacgttga cgaacacgag cgcgaggccg tattgcgcga gcaggccggt catcccttcc
     4561 aactgatctc tccccgctta gggaatgatt tacgacatct tatcgcacag cgatcagcga
     4621 tcagcgatca gcgatcagcg tgagaccaag agcggctcta agcttcaagc tgagggctga
     4681 cggctgacag ctgaccgctc agtcaaagcg gtaggcatcc atgctgatca cgccagactc
     4741 gatgctcgta tgcaggcgtc gcgcggcggc gtccggaccg ccggcaccga gggcgacgaa
     4801 caatggaatc aggtgttcat cggtaggatg attgcgcacc gcgtgcggcg ctctctcacg
     4861 gtaggcaact agatcatcca tgcgccgcga cttcaccgcg tcggccagcc attcattgaa
     4921 ctcgacggtc cacg
//
LOCUS       DBNGWZ010000389        18334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2438254, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000389 DBNGWZ010000000
VERSION     DBNGWZ010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2438254"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16CX_11410"
     CDS             complement(<1..663)
                     /locus_tag="AC16CX_11410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR3013153.1"
                     /translation="MNKLVLKPGREKSLKRCHPWVFSGAVAKVQGEPGPGETVGVWSA
                     TGEFLAVAAYSPKSQIIARVWGWKERAIDRGFLNELIARAVEQRRPLFDGSDTDAFRL
                     IHGESDGLPGVVADRYGDTIVLQLTSAGAERWRDAIADALLQASGAARIWERSDAEVR
                     ALEGLAPVTAALRGAREPTRIAVTENGLRFEIDLEHGHKTGFYLDQRDNRRRLRELAR
                     GRD"
     gene            complement(757..1677)
                     /gene="xerC"
                     /locus_tag="AC16CX_11415"
     CDS             complement(757..1677)
                     /gene="xerC"
                     /locus_tag="AC16CX_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015856086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR3013154.1"
                     /translation="MGANSKPEKPAKPAVHPLLRGYLDHLGNERRLSRHTLENYGRDI
                     AALLVLSGDTPLKQLQVHHVRRHVAQLHSRGLSGKTLARMLSAWRGFFHYLARDHGFK
                     HNPCVGIRPPRSPKRLPHALSPDEAGRLMEIGATDPLAVRDKAIMELLYSSGLRLSEL
                     TGLAQDDVNFRDATVRVTGKGAKTRIVPVGSYALAALTAWLPAREQLAHQGEKALFIG
                     RDGNALGPRAVQARLKHWALKLGLADKVHPHALRHSFASHLLQSSGDLRAVQEMLGHA
                     SISTTQVYTHLDFQHLAKAYDAAHPRAKKK"
     gene            complement(1758..2405)
                     /locus_tag="AC16CX_11420"
     CDS             complement(1758..2405)
                     /locus_tag="AC16CX_11420"
                     /inference="COORDINATES: protein motif:HMM:NF016243.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HIR3013155.1"
                     /translation="MKPEEVAGYLKEHPEFFEHYADLMAEIYIPHPHGGRAIPISERQ
                     ILTLREKNKQLEGKLREVIQFGEENDAISEKVHRISVALLAASDIRGVLNTVYLNLRE
                     DFSVPHVALRVWRANGHADLPEFKPMSAASREFTTSLTNPYCCGHAMVDTAGLFGEAS
                     AHLRSFSYLPLRDNETFGLLALASGDPQRFYPEMGTLYLKRLGDLVGAAIARHLK"
     gene            complement(2504..3334)
                     /gene="dapF"
                     /locus_tag="AC16CX_11425"
     CDS             complement(2504..3334)
                     /gene="dapF"
                     /locus_tag="AC16CX_11425"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3013156.1"
                     /translation="MRLKFTKMHGLGNDFVVLNALTWPLSLTPQQLRLLADRHFGVGC
                     DQILQVEPPRAPDTDFYYRVFNSDGGEVEQCGNGARCFVRYVRDRGLTTKTEIRVGTR
                     SGVIVPRLEEDGSVTVDMGLPEFEPVRIPFDAGARAPTYGLDLDGRSVEVSVLSMGNP
                     HAVQIVTDVERAPVATEGPLIERHPRFPQRVNAGYMQIVSRRRILLRVYERGAGETLA
                     CGTGACAAVAAGITRGLLDREVTVTTRGGDLGISWAGEGRPVMMTGPAVTVFEGEMEI
                     "
     gene            complement(3335..3748)
                     /locus_tag="AC16CX_11430"
     CDS             complement(3335..3748)
                     /locus_tag="AC16CX_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3013157.1"
                     /translation="MFENPKVEERRALLKRVKTIAVVGLSPNPTRPSFGVARAMQGFG
                     YTIIPVHPVAREVLGAEAFRRLADIVVPIDLVNVFRRAEHIDGVVGECLALGLKNLWI
                     QEGIVNEPAALRAREGGMTVIMDRCIYRDYIQYCS"
     gene            complement(3766..4284)
                     /locus_tag="AC16CX_11435"
     CDS             complement(3766..4284)
                     /locus_tag="AC16CX_11435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3013158.1"
                     /translation="MENKVRIEPLEPDDVKALVALAREIWYAHYPAIIGTAQIEYMLG
                     QRYEPAVVRAELGRDGIWWDKLMAGEKMAGFASYFLTEAPGEMKLDKVYVHPRHQRHG
                     YGGMMIARACEVARQRGCSRLVLAVNKNNRSAIAAYLKYGFRVADAVVKDIGGGFVMD
                     DYVMEKPVIGDK"
     gene            complement(4427..5386)
                     /locus_tag="AC16CX_11440"
     CDS             complement(4427..5386)
                     /locus_tag="AC16CX_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3013159.1"
                     /translation="MSKLRAAVIGAGRLGRHHAAKYAARDDVVLVAVADLLEANAREL
                     AAALGCRAVRDFREVLHDVDLVSITVPTVSHYEVARACLEAGIHVLVEKPITRSLDEA
                     DALIALAARRNVRLAVGHVERFNPAFAAMREMLARPVFIESERLAEYQPRVTDVDVVL
                     DLMIHDIDLALALTDAEIATVIACGYKVLTDSIDIATAHIEFADGTVAILSASRVSQA
                     PVRKLRAFARDAYASADLQGASLRIARRANGMQIASEERTFPRPDALNAEIDSFVAAV
                     RGSRTATVSGAEGRRALALALEVSARIDERLARLPSPAPQERA"
     gene            complement(5425..6294)
                     /locus_tag="AC16CX_11445"
     CDS             complement(5425..6294)
                     /locus_tag="AC16CX_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR3013160.1"
                     /translation="MLTRVALGLVWLLRFLPLAVLAPLGRALGLLGYALVAERRRVTL
                     TNLGLCFPHWSETQRRRLARRHFQAVGRAVLEHGILWWSSAKRVQRLVRIEGLEHWQA
                     VADRPVIWLAPHFIGLDMGGVRLGSEYRVVSVYSHQKDPLFDAVLYRGRTRFVMPELF
                     SRQQGIRPVVRALRKGLPFYYLPDMDFGGRDSIFVPFFGVPAATITALSRIASLAGAA
                     VVPAVTRQLPGTGGYVLSFYPAWRDFPSDDIERDTRRMNAFIEERVLEMPEQYFWLHK
                     RFKTRPPGEARYY"
     gene            complement(6356..7240)
                     /locus_tag="AC16CX_11450"
     CDS             complement(6356..7240)
                     /locus_tag="AC16CX_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3013161.1"
                     /translation="MFVALFRLLARLPLSWLQGAGAVLGWLVYSASPSYAARLRENLY
                     ASGVCDGEERCQAMLRAAVAETGKGVTELIAIWFGSEQSVARLVVECERWDIVEAARA
                     RGNGIIFLTPHLGCFEISALYGAQRMPLTVLYRPPKKRWIEPLMVAGRQRWQAVVAPA
                     NLKGVRMLYKALARGEAVGLLPDQAPGVGEGVWADFFGRPAYTMTLVRRLQSASGAAV
                     IMAFAERLPASRGYRLHLEELSTSPLDEAALNRAIEAQVRRCPEQYLWSYNRYKTPAG
                     AEPPPAKSKVESRKSKVS"
     gene            7309..8472
                     /gene="metK"
                     /locus_tag="AC16CX_11455"
     CDS             7309..8472
                     /gene="metK"
                     /locus_tag="AC16CX_11455"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3013162.1"
                     /translation="MSDFLFTSESVSEGHPDKIADQISDAVLDAVLAQDRHGRVAAET
                     LVATGLVVMAGQITTHAAVNYGDLARDVIRRIGYDDSSMGFDYRSCAVLTAFDRQSAD
                     IALGVDEGKGLDLDQGAGDQGLMYGFACNETPQLMPMPIYYAHRLMERHGELRRDGRL
                     PWLRPDAKSQVSVRYVDGKPHHIDTVVISTQHREDISHEPLTEAVIEEMVKPVLPKNM
                     ITQETRYLINPTGRFVIGGPLGDTGLTGRKIIVDTYGGYSRHGGGAFSGKDPSKVDRS
                     AAYAARHVAKNIVSAGLAKKCEIQVAYAIGVARPVSVLVETFGTGRIPDDKLAKLVEK
                     HFDLRPKGIIRALDLLRPIYFKTATYGHFGRDEPEFTWEATDKAAALKADAGV"
     regulatory      8670..8738
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            8776..10194
                     /gene="ahcY"
                     /locus_tag="AC16CX_11460"
     CDS             8776..10194
                     /gene="ahcY"
                     /locus_tag="AC16CX_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR3013163.1"
                     /translation="MNAAVTESKPADYFVADIKLAGFGRKEIAIAETEMPGLMAVREE
                     YRGKQPLKGARIAGSLHMTIQTAVLIETLVRLGAQVRWASCNIFSTQDHAAAAVAAAG
                     IPVFAYKGESLAEYWDFTHRIFEWADGGTPNMILDDGGDATLLATLGARAEKDPALVA
                     RPGNEEETVLFSAIRKRLESKPGWYSKALAGIRGVTEETTTGVHRLYQMEKEGRLPFP
                     AFNVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVIAGYGDVGKGCAQSLRG
                     LGANVWVTEIDPICALQAAMEGYRVVTMDYAADKADIFVTATGNVRVITHEHMKRMKH
                     NAIVANIGHFDSEIDIASLKQYPWENIKPQVDHVLFPDGKRLIVLAEGRLVNLGCATG
                     HPSFVMSNSFTNQVLAQIELFGNAARYEKKVYVLPKQLDERVARLHLKKLSADLTVLT
                     EEQCRYIGVNIDGPYKPDYYRY"
     gene            10287..11132
                     /gene="metF"
                     /locus_tag="AC16CX_11465"
     CDS             10287..11132
                     /gene="metF"
                     /locus_tag="AC16CX_11465"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR3013164.1"
                     /translation="MQSQKRHPRIFSFEFFPPKTAEGKEKLRATWKQLGQLKPRFFSC
                     TYGAGGSTREGTLDTVLEIRSAGFEAAPHISCVASSRADLKAQLEKYRSHGIRHVVAL
                     RGDLPSGLASLGELRYANELVAFIRELTGDWFHIEVACYPEYHPETRSAADEIRHFKG
                     KVDAGANSAITQYFYNADAYFRFVDDCEAAGITVPIVPGVMPIGNFAQLARFSDACGA
                     EIPRWLRHRLESYRDDTASIRSYGLDVVTRLCDDLLSAGAPGLHFYTLNQAGLTTTIW
                     QRLGL"
     gene            11366..12604
                     /locus_tag="AC16CX_11470"
     CDS             11366..12604
                     /locus_tag="AC16CX_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3013165.1"
                     /translation="MNAEQSNTQANPQEPPHKALIPEVKARSPQEAAKLLAEHPAPVI
                     AATLLEINPGIAQDILSELSGGLMDAVLHAVAPEMALQWQRNQTYPQNSVGRLMEPAY
                     AVFHPGMTVAQTVERLRPLIKVAFITYGYVIDESGKLGGLITMRDLLFADDDARLESL
                     MLRDVFTLRPETPLNEAMKLVLDRHYPVYPVCDGNGVLIGLVRGQAMFEEQAIEITAQ
                     YGSLVGVEKEERLATSLKQSFKFRHPWLQLNLLTAFVAGAVVALFQDTVDRLVILAVF
                     LPVLAGQSGNTGCQALAVTLRGLTLGELKTGKERNLVAKEAWLGFYNGALTGLVAGLG
                     MLAMAIYQGNPNAILLAVVVWMALVGACVTSGVCGAMIPLTLKRFGLDPATASSIFLT
                     TATDVVSMGMLLGLAALLIR"
     gene            complement(12687..13466)
                     /locus_tag="AC16CX_11475"
     CDS             complement(12687..13466)
                     /locus_tag="AC16CX_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013166.1"
                     /translation="MTIVTVNGADLDIVQIGSGRDLVLLHSLLTDRSAFDRVVPALSK
                     SRRLTLVNLPGYGASSPAGAAVEDYADRIAAMLAAMRLPPQTDVLGNGFGGFVSIALA
                     ARHGAKFDRLIVADALATFPEPAKQPLRNLAAKVAQEGMAGALDVAIRRMFPEAFIAA
                     NPQIVAERKRVLEREADPACFQQACVALARLDFTPVLGKLRNPALVMVGALDQTTPPV
                     LARELAAKIAGARFLEIAGCGHCPQIERPEAFVEAVNAFLA"
     gene            complement(13534..15072)
                     /locus_tag="AC16CX_11480"
     CDS             complement(13534..15072)
                     /locus_tag="AC16CX_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3013167.1"
                     /translation="MIIFRAMNCAERLVALFGTQAEVARQLRLDRAVVSNWVKSGYVP
                     ARWAMEVEALSSGQITAVEVLNEANTRKPLRFKSRPDGESLFGSPLTGKDVMSDYAPV
                     KRITSFHPPQRTLMGPGPTEIHPRVLTTMSQPAIGYLDPVFVEMMEELKALLRYVYQT
                     KNPLTFPISGPGSVGMEYCFVNLVAPGDKVIVCRNGVFGGRMIENVERMGGVPVVVDD
                     KWGEPIDPDKLEDALKKNRDARVVAFVHAETSTGVQSDAKTLVGIAHRYDALTIVDAV
                     TSLGGTPVLVDEWGIDAIYSASQKCLSCTPCLSPVSFSERVVEYVKARKDKIHSWFMD
                     MNLLLGYWGATNRTYHHTAPTNALFALHEALLLIHEEGLENSWARHRRHHTALKAGLE
                     AMGLKFLVKPEYQLPQMNAVLCPEGVEEAAVRRTLLTEFNLEIGAGLGPLAGKIWRFG
                     IMGYSCRPDNVMLCLAALGSVLSDMGLPVHVGDAEAAAHAAYAAMHAKAAQAKAKTKA
                     RVAA"
     gene            15168..16118
                     /locus_tag="AC16CX_11485"
     CDS             15168..16118
                     /locus_tag="AC16CX_11485"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR3013168.1"
                     /translation="MLKEFNNLNELEAAFPTEESCIEHYAEIRWPRGVACPHCGVMNV
                     YELARTGYYKCAESRCGKKFSWRYGSIFADAKIELRTWFRAIFLMTSHKKGISSMQLA
                     RDLGITQKSAWHMLHRVREATMTEEFRRPLLTGTIQADETLIGGKEQFKHANKRTPFT
                     EGRGSMRTKTMVFGMVQPGGELRMQKLPVMSRRAIERIVKKNVRLGSTVHTDDATHYL
                     WMNAHYEHSLVKHRMGEYVAKDGTTTNAIEGAFSHFKRTVTGVYHKVSDEQIDRYLGM
                     FSWRWTRRKMAEGERVNALLKFTPGHQLSYRKLTRKDEVQ"
     gene            16407..17084
                     /gene="dcd"
                     /locus_tag="AC16CX_11490"
     CDS             16407..17084
                     /gene="dcd"
                     /locus_tag="AC16CX_11490"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3013169.1"
                     /translation="MLMTDRQIAEARTKGDVKIDPFEDKQLQPASYDLRVGEQGITTT
                     SKKKINIKADGFLVIHPGDFAVVLVHEEIRLGPQYAARFGLRSKYARRGLVATTGLQV
                     DPGYYGNLIVGITNLTPRPITLPYKDDLLSIEFHRLEEPASKPYNGPYQGRKELGPEE
                     IEFIIESEGMALSEVLTTLRSLSANVGVLTNDVTALTNEMKYYKWVVPALLAIGMTII
                     GGIVAIK"
     gene            complement(17397..17876)
                     /locus_tag="AC16CX_11495"
     CDS             complement(17397..17876)
                     /locus_tag="AC16CX_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013170.1"
                     /translation="MSKVSPNSEKVMKLLLEGKIEQARKFVEKDIAKQIEAKRAELTE
                     LNRERSVLSNMVMLQKGEQDATPTSTKISPPQKRVIPKEERSVLIKQYAHEAARQAAN
                     QTITLQAVVDQLEADGIDLGTDVPGTAIGNVLFKSNDWRRIERGIFQYVGAQLDLRH"
     gene            complement(18099..18302)
                     /locus_tag="AC16CX_11500"
     CDS             complement(18099..18302)
                     /locus_tag="AC16CX_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013171.1"
                     /translation="MSRHDREVVKRRSQAVNEVVRAPDVLARMAAIGMEPVGPAPEQY
                     EKNLREEHERLGTLIRRIGLRID"
BASE COUNT         3480 a         5863 c         5665 g         3326 t
ORIGIN      
        1 gtcgcgcccg cgcgcaagct cccgcaacct ccggcggtta tcgcgctggt cgagatagaa
       61 accggtcttg tgcccgtgct cgaggtcgat ctcgaaccgg aggccgtttt cggtgaccgc
      121 gatccgcgtc ggctcccgcg cgccacgcag cgccgcagtc acgggcgcca gcccttcgag
      181 cgctctcact tcggcgtccg agcgctccca gatacgcgcc gcgccgctcg cctgaagcag
      241 cgcgtccgca atcgcatcgc gccagcgctc ggcacccgcg ctggtcagtt gcagcacaat
      301 cgtatcgcca tatcgatcgg cgaccacgcc cggtaggccg tccgattcgc cgtgaatcaa
      361 ccggaaagcg tcagtgtcag atccgtcgaa caacggtcgg cgttgttcta cggctcgagc
      421 aatgagttcg ttaaggaacc cacggtctat cgcgcgttct ttccagcccc agacccgggc
      481 gatgatctgg gacttcgggc tgtaggcggc aacggccagg aattccccgg tggcggacca
      541 gacacccacg gtttcgcctg gacccggttc cccctgtacc ttcgccaccg cccccgagaa
      601 cacccacggg tggcagcgct tgagcgactt ctcgcggccc ggtttgagta ccagtttgtt
      661 cattgttaag ccgccagttc ccccgcgttg agcgacgatg gactcgtccg ttccagcgcg
      721 cggagttaaa ccggcgcctc gtgtccggct ttaacgctat ttctttttcg cccggggatg
      781 cgccgcgtcg taggctttcg cgagatgctg aaagtccaag tgcgtgtaga cctgcgtcgt
      841 cgagatgctc gcgtgcccca gcatctcctg caccgcgcgc aggtccccgc tcgactgcag
      901 caggtgcgag gcgaaggagt gccgcagcgc gtgcggatgc accttgtcgg cgagcccgag
      961 cttcaacgcc cagtgcttga gccgcgcctg caccgcgcgc ggacccaggg cattgccgtc
     1021 gcgcccgatg aacagcgcct tttcgccttg gtgcgcaagt tgttcacgcg ccggtagcca
     1081 ggccgtgagc gccgcgagcg cgtagctgcc caccggcacg atgcgcgtct tggcgccctt
     1141 gccggtcacc cgcaccgtcg cgtcacggaa gttgacatca tcctgcgcga gcccggtcaa
     1201 ctcggagagc cgcaggccgg aggagtaaag cagctccatg atggccttgt cgcgcacggc
     1261 aaggggatcg gtggcgccga tctccatgag gcgtcccgcc tcgtcgggcg agagcgcgtg
     1321 cggcagccgc ttgggcgacc tggggggacg gatgccgaca caggggttgt gcttgaagcc
     1381 gtgatcgcgc gcgaggtagt ggaagaagcc gcgccacgcg gagagcatgc gcgcaagcgt
     1441 cttgccgctc agcccgcgcg aatgcagctg ggccacgtgg cggcgcacgt ggtgcacttg
     1501 aagctgcttg agcggggtgt cgcccgagag cacgagcagc gctgcgatgt cgcgtccgta
     1561 attctccagg gtgtgacggc tcagccggcg ctcgtttcca aggtggtcca gatagccgcg
     1621 caggagcgga tgcacggccg gtttggcggg tttttcaggc tttgagttag cgcccactta
     1681 catcccgccg caacgggcca attcactgta ccgcagggct ttttcagctc ttcccctcac
     1741 ccctcactcc tcacccctta cttgaggtgc cgcgctatcg cggcaccgac cagatcgccg
     1801 agccgtttga gatagagcgt gcccatctcg gggtagaaac gctgcggatc cccgctcgcc
     1861 agcgcgagca gtccgaaggt ctcgttgtcc cgcagcggca agtaggaaaa ggaacgcagg
     1921 tgcgcgctgg cttcaccgaa cagacccgcg gtatccacca tggcgtggcc gcagcaatag
     1981 gggttggtga ggctcgtcgt gaattcgcgc gaggcggcgc tcatcggctt gaattccggc
     2041 aggtcagcat ggccgttggc ccgccatacc cgcaacgcca cgtgcggcac cgagaaatcc
     2101 tcgcgcaggt tgagatagac cgtgttcagc acgccgcgga tgtcggaggc cgcgagcagg
     2161 gccaccgaaa tgcggtgcac cttttcgctg atcgcgtcgt tctcctcgcc gaactggatc
     2221 acctcgcgca gcttgccctc gagttgttta ttcttctcgc gcagggtcag gatctggcgc
     2281 tcgctgatcg ggatcgcgcg cccgccgtgc ggatgcggga tgtagatctc cgccatcaga
     2341 tcggcgtagt gctcgaaaaa ttccgggtgt tccttgagat agcctgcgac ctcttccggt
     2401 ttcatcgttc tccctgtcgt tagctttcag cgttcagcga tcagctttca gcttgataaa
     2461 accgtgttgc tttggctgac agctgacggc tgggtgctga ccgctatatc tccatctcgc
     2521 cttcgaagac cgtgaccgcc ggcccggtca tcatcaccgg cctgccctcc cctgcccatg
     2581 atataccgag gtcgccgccg cgcgtcgtta ccgtgacctc gcgatcgagc aacccgcgcg
     2641 tgatgccggc ggcgacggcg gcgcaggcgc ccgtgccgca cgcgagcgtc tcacccgcgc
     2701 cgcgctcgta gacgcggagc aggatacggc ggcggctgac gatctgcatg tagccggcgt
     2761 tgacccgctg cgggaagcgc ggatggcgct cgatcagcgg gccctcggtc gcgaccggcg
     2821 cgcgctcgac gtcggtgacg atctgcaccg catgcggatt gcccatggac aagacgctca
     2881 cctccacgct gcggccgtcc aggtcgagcc cataggtcgg tgcccgcgcg ccggcatcga
     2941 agggtatgcg caccggctcg aactccggta accccatgtc gaccgtcacg ctgccgtcct
     3001 cctccagccg cggcacgatc accccgctgc gggtaccgac ccggatctcg gtcttggtgg
     3061 tgagcccccg gtcccggaca taacgcacga agcagcgtgc cccgttccca cactgttcca
     3121 cctcgccgcc gtcggagttg aaaacgcggt agtagaagtc ggtatcgggg gcgcgcggcg
     3181 gctcgacctg gaggatctga tcgcaaccca ccccgaaatg ccggtcggct agcaagcgga
     3241 gctgctgagg ggtcagggaa agcggccagg ttagggcatt cagaaccacg aagtcattgc
     3301 ccaatccgtg cattttagtg aattttaggc gcatttagct gcaatattgt atgtaatcgc
     3361 ggtagataca gcgatccata atcaccgtca tgcccccttc ccgagcccgc aaagcggccg
     3421 gctcgttgac gataccttct tgaatccaaa ggtttttcag gcctagcgct aggcactcgc
     3481 ccaccacgcc gtctatgtgc tcagcccggc ggaaaacgtt gaccaagtct atcgggacca
     3541 cgatatcggc taaccgacgg aaggcctcgg ctcccaagac ctcccgagcg acaggatgca
     3601 ccgggatgat ggtgtaaccg aaaccctgca tcgcccgcgc cacgccgaaa ctggggcggg
     3661 tggggttggg tgaaagcccc accaccgcga tcgttttcac gcgcttcagg agggcgcggc
     3721 gttcctcgac cttggggttt tcaaacatgc tgggttcgac acctatcact tatcacctat
     3781 cacgggtttt tccatcacgt agtcgtccat cacgaagccg ccgccgatat ccttgaccac
     3841 ggcatcggcc acgcgaaacc cgtatttcag gtaggcggca atcgcgctgc ggttgttctt
     3901 gttcaccgcc agcacgagcc ggctgcaacc gcgctgccgc gccacctcgc atgcgcgtgc
     3961 gatcatcatg ccgccgtagc cgtggcgctg atgccgcggg tgcacgtaaa ccttgtcgag
     4021 cttcatctca ccgggcgctt cggtcagaaa gtacgaggca aatccggcca ttttctctcc
     4081 ggccatcagc ttgtcccacc agatgccgtc gcgcccgagc tcagcccgca ccaccgccgg
     4141 ctcatagcgc tgcccgagca tgtattctat ctgcgcggtg ccgatgatgg cgggatagtg
     4201 ggcgtaccag atctcccggg cgagcgcgac gagcgctttc acgtcgtcag gctcgagcgg
     4261 ctctatgcgc accttgtttt ccattccgcg atccaatcga ggttcccggc gcaacgggcg
     4321 cgaaggaaaa gcagcggcag atttcctgct tcgcgctttg atgatcaagt gagtattacc
     4381 ccgcccgtcg ccgcgtgcgc gacgccagga tcgggcatcg ggacgttcac gcccgctcct
     4441 gcggtgccgg cgagggcagg cgcgcgagcc gctcgtcgat gcgcgcgctc acttcgagcg
     4501 cgagcgccag ggcccggcgc ccctccgctc cggacaccgt cgctgtccgg ctcccgcgca
     4561 ctgcggcaac gaacgagtcg atctccgcgt tcagggcatc cggtcgagga aatgtgcgct
     4621 cctcgctcgc gatctgcatt ccgttcgctc ggcgcgcgat tctcaggctg gcgccctgca
     4681 ggtccgcgga agcataggcg tcacgggcga acgcgcgcag cttgcgcacc ggcgcctggc
     4741 tgacacggct cgccgacagg atggccaccg tgccgtcggc gaattcgatg tgcgcggttg
     4801 caatgtcgat cgaatcggta aggaccttgt agccgcaggc aatgaccgtc gcgatctcgg
     4861 cgtccgtgag cgccagcgcg agatcgatat cgtggatcat caggtcgagc acaacatcga
     4921 catccgtgac cctcggctga tactcggcga gccgttccga ttcgatgaag accgggcgcg
     4981 cgagcatctc ccgcatggcg gcaaaggccg ggttgaagcg ctcgacgtgg ccgaccgcga
     5041 ggcgcacatt gcgtcgcgcg gcgagggcga tcagcgcatc ggcctcgtcg agcgagcggg
     5101 tgatcggctt ctccacgaga acgtgtatgc ccgcttcgag gcaagcgcgc gccacctcgt
     5161 agtggctcac ggtcggaacc gtgatcgaaa ccagatcgac gtcgtgcaat acctcgcgga
     5221 aatcgcgcac ggcgcggcaa ccgagcgccg ctgcgagttc gcgcgcattg gcctccagca
     5281 aatcggccac cgcgacgagc accacgtcgt cgcgggcggc atatttcgcg gcatggtggc
     5341 gccccaggcg cccggccccg atgacggcgg cgcgcagctt gctcatgggc gaaattgtct
     5401 cccagtcacg gctcctccgc cacgtcagta atagcgggcc tcccccggag gacgggtctt
     5461 gaaacgcttg tgcagccaga agtattgctc cggcatctcg agcacgcgct cctcgatgaa
     5521 ggcgttcatg cgccgggtgt cgcgttcgat gtcatcgctc gggaaatcgc gccacgccgg
     5581 gtaaaagctg agcacgtagc cgcccgtccc cggcagctgg cgcgtcactg cgggcaccac
     5641 ggcggcgccc gcaaggctgg cgatccgcga caaggccgtg atcgtcgctg ccggcacgcc
     5701 gaagaacggc acgaagatcg agtcgcggcc gccgaaatcc atgtccggca ggtaatagaa
     5761 cggcaggcct tttcgcaacg ccctcaccac cggccgtatg ccctgttgcc gggagaacag
     5821 ttcgggcatc acgaagcgag tccgtccccg gtagaggacc gcgtcgaaca gcggatcctt
     5881 ctggtggctg tagacggaga ccacgcgata ctcggagccc agacggacgc cccccatgtc
     5941 gagcccgatg aaatggggcg cgagccagat gaccggacgg tcggcaaccg cctgccagtg
     6001 ttcgaggcct tctatccgca ccagccgctg cacccgcttc gccgatgacc accacaggat
     6061 gccgtgctcg aggacggcgc gcccgaccgc ctggaagtgg cgccgtgcga ggcgccgccg
     6121 ttgtgtttcg ctccagtgcg ggaagcacag cccgagattg gtgagcgtta cccgcctgcg
     6181 ctcggcgacc agcgcataac ccagcaaacc cagggcacgg cccaggggcg ccagcacagc
     6241 aagcggcagg aagcgcagca gccagacgag accgagcgcg acccgtgtta acacgtcgcg
     6301 ctcttaagtc gaaaggcgaa aggcgaaagg cggaagggca gaaaagcgac catggctaag
     6361 acactttcga cttccgactt tcgactttcg attttgccgg cggtggctca gctcccgccg
     6421 gcgtcttgta gcggttgtag ctccacaaat actgctccgg acagcgccgc acctgggcct
     6481 cgatcgcgcg attgagcgcg gcctcgtcga gcgggctcgt ggagagctcc tccaggtgca
     6541 gacgatagcc gcgactggcg ggcaggcgct cggcgaacgc catgatcacc gcagcgccgc
     6601 ttgcgctctg cagccgcctg acgagcgtca tggtgtaggc gggccggccg aagaagtccg
     6661 cccacacgcc ttcgcccacg cccggcgcct gatcgggcag caaccccact gcctcgccgc
     6721 gcgccagcgc cttgtagagc atgcgcacgc ccttgaggtt tgccggcgcc accaccgctt
     6781 gccagcgttg ccgtcctgcg accatcagcg gctcgatcca gcgctttttc ggcggccggt
     6841 agagcacggt gaggggcatg cgctgcgcgc cgtagagcgc cgagatctcg aaacagccga
     6901 ggtgcggcgt gagaaaaatg atgccgttgc cgcgcgcgcg cgcggcttcc acgatatccc
     6961 agcgctcgca ctccacgacc agccgcgcaa cgctttgttc acttccgaac cagatcgcga
     7021 tcagctcggt gacgcccttg ccggtttcgg ccaccgcggc gcgcaacatc gcctggcacc
     7081 gctcttcgcc gtcgcatacg ccgctcgcgt agagattttc cctgagtcgc gccgcgtacg
     7141 agggtgacgc cgagtacacc agccagccga gcaccgcccc cgcgccttgc agccacgaaa
     7201 gcggcagccg cgcgagcagc cggaacagcg cgacaaacat gaatttggct ttgtaaatcg
     7261 agggttatct gctattctaa gcgccttttt ccgtttcgag aaaaataaat gagcgatttc
     7321 ctgttcactt ccgagtccgt atccgaaggg caccccgaca agatcgcgga ccagatttcc
     7381 gacgcggtgc tcgatgcggt gctcgcgcag gaccggcatg gccgcgtcgc cgcggagacg
     7441 ctggtcgcga ccggcctcgt cgtgatggcc ggccagatca ccactcacgc ggcggtcaat
     7501 tacggcgacc tcgcgcgcga cgtgatcagg cgcatcggct acgacgactc gtcaatgggc
     7561 ttcgattacc gctcgtgcgc ggtcctcacc gctttcgacc ggcagtccgc cgacatcgcg
     7621 ctgggcgtgg acgagggcaa ggggctcgac ctcgaccagg gcgccggcga ccagggcctc
     7681 atgtacggct tcgcctgcaa cgagacgccg cagttgatgc cgatgccgat ctactacgcg
     7741 caccggctga tggaacgtca cggcgaattg cggcgcgacg gccggttgcc gtggctgcgt
     7801 cccgacgcca agtcccaggt ttccgtgcgc tacgtggacg gcaaaccgca ccacatcgac
     7861 accgtggtca tctcgacgca gcaccgcgag gacatctcgc acgagccgct caccgaggcg
     7921 gtgatcgagg agatggtgaa gccggtgctc ccgaagaaca tgatcacgca agagacgcgc
     7981 tatctcatca accccaccgg tcgcttcgtc atcggcgggc cgctgggcga caccgggctt
     8041 accggccgca agatcatcgt ggacacctac ggcggctatt cgcgccacgg cggcggcgcg
     8101 ttctccggca aggatccctc caaggtggac cgctcggcgg cgtatgcggc ccggcacgtc
     8161 gccaagaaca tcgtctccgc ggggctcgcg aagaaatgcg agatccaggt cgcctatgcg
     8221 atcggcgtcg cgcgcccggt gagcgtgctg gtcgagacat tcggcaccgg caggatcccc
     8281 gacgacaagc tcgcgaagct cgtcgagaaa cacttcgacc tgcgccccaa gggcatcatc
     8341 cgggcactcg acctgctcag accgatctac ttcaagaccg cgacctacgg ccacttcggc
     8401 cgcgacgagc cggagttcac gtgggaggcc accgacaagg cggcggcact caaggccgac
     8461 gcgggagtgt aaagagcaga aacccgatta gccgcagata aacgcaccca ttagccggcc
     8521 ggcgtcaaga gtgatacaca cggattaaat cccctgctaa tgccctcttc gggtttgcaa
     8581 gcatctgtgt tcatctgcgt gtatctgcgg tttcattagg ggtttcaggc ggactggccg
     8641 gaggaccggc ggctgcggta taattccgcc tcgctgagga gcgttgcgac cggggaatcc
     8701 cggccaggct cagcaaaaat caacggcgct cgcatcggaa acgatgcgag cgctttgctt
     8761 ttaggagctc gatccatgaa cgcagccgtg acggagtcga agccggccga ctatttcgtc
     8821 gccgacatca agctggccgg cttcggccgc aaggaaatcg ccatcgccga gaccgagatg
     8881 cccggcctca tggcggtgcg cgaggagtac aggggcaagc agccgttgaa gggcgcgcgc
     8941 atcgccggtt ctctgcacat gacgatccag accgcggtgc tgatcgagac cctggtcagg
     9001 ctcggggcgc aagtgcgctg ggcctcgtgc aacatcttct cgacgcagga tcacgccgcg
     9061 gccgccgtcg ccgccgccgg catcccggtg ttcgcctaca agggggagag cctggccgag
     9121 tactgggatt tcacgcaccg catcttcgaa tgggcggacg gcggcacgcc caacatgatc
     9181 ctcgacgatg ggggcgacgc gacgctgctc gcgacgctcg gcgccagagc ggagaaggac
     9241 cctgcgctcg tcgcgcggcc gggcaacgag gaagagaccg tgctcttcag cgcgatccgg
     9301 aagcggctgg aatccaaacc cggctggtac tcgaaggcgc tcgcgggcat ccgcggcgtg
     9361 accgaggaaa ccaccaccgg cgtgcaccgc ctctaccaga tggaaaagga ggggcggctg
     9421 ccgttccccg cgttcaacgt caacgattcc gtcaccaagt cgaaattcga caacctctac
     9481 ggctgccgcg agtcgctggt ggacggcatc aagcgcgcca ccgacgtcat gatcgcgggc
     9541 aaggtcgcgg tgatcgccgg ctacggcgac gtcggcaagg gctgcgcgca atcgctgcgc
     9601 gggctcgggg ccaacgtatg ggtgaccgag atcgacccga tctgcgcgct gcaggcggcg
     9661 atggagggct accgcgtggt gaccatggat tacgcggccg acaaggcgga catcttcgtc
     9721 accgcgaccg gcaacgtgcg ggtcatcacg cacgagcaca tgaagcgcat gaagcacaat
     9781 gcgatcgtcg ccaacatcgg gcacttcgat tccgagatcg acatcgcgag cctgaagcag
     9841 tacccgtggg aaaacatcaa gccgcaggtg gaccacgtgc tcttccccga cggcaagcgg
     9901 ctcatcgtgc tcgccgaggg gcgcctggtg aacctcggtt gcgccaccgg acatccgagc
     9961 ttcgtgatgt ccaactcctt caccaaccag gtgctggcgc agatcgagct tttcggcaac
    10021 gcggcccgct acgagaagaa ggtctacgtg ctgcccaagc agctcgacga gcgcgtggcg
    10081 cggctgcacc tcaagaaact cagcgccgac ctcacggtcc tgaccgagga gcagtgccgc
    10141 tacatcgggg tgaacatcga cgggccgtac aagccggact actaccgtta ttaaccgcgt
    10201 gaggtgtaag gcgtgaggtg tgaggagaaa aaccttatgc tccccgccta acgcctcacg
    10261 cctgacgcct cactcgccac cggacgatgc agtcgcagaa gagacatccc cggattttca
    10321 gcttcgaatt ttttcccccg aaaacggcgg aaggcaagga gaagctgcgc gcgacctgga
    10381 agcagctcgg gcagttgaag cccaggttct tctcgtgcac gtacggcgcg ggcggctcca
    10441 cgcgcgaggg cacgctcgac actgtgctcg agatccgctc cgccggtttc gaggcggcgc
    10501 cgcacatctc ctgtgtcgcc tcctcgcgcg cggacctcaa ggcccagctc gaaaagtacc
    10561 ggtcgcacgg catccggcac gtcgtcgccc tgcgcggcga cctgccctcg gggctcgcct
    10621 cgctcggcga gctgcgctac gcgaacgagc tggtcgcctt catccgggag ctcaccggcg
    10681 actggtttca catcgaggtc gcctgctatc ccgagtacca cccggagacg cgctcggccg
    10741 ccgacgagat caggcatttc aagggcaagg tggacgccgg cgccaattcc gcgatcaccc
    10801 agtacttcta caacgcggac gcctacttcc gcttcgttga cgattgcgag gcggcgggaa
    10861 tcacggttcc catcgttccc ggcgtcatgc cgatcggaaa tttcgcccag ctcgcgcgct
    10921 tctcggatgc ctgcggcgcc gagatcccgc gctggctgcg ccaccggctg gaaagctacc
    10981 gcgacgacac cgcctcgatc cgttcctatg ggctcgacgt cgtgacgcgc ctgtgcgacg
    11041 acctgctctc cgccggcgcg ccgggcctgc atttctacac cctcaaccag gccggactca
    11101 ccaccactat ctggcaaaga ctgggtctct gataaggggg attcggggct cgggactcgg
    11161 agctcgctcc ctccggctcc cggaagaacc cgggttgttt caagtcccaa gtcccgagtc
    11221 tccagtaccg cttttacaat ttctggggca caattaccgt taaagcggac ggcgctgccg
    11281 ccgctttaac tgcgccggtt cgaacggaca cccagtgccg ctcaaccggc gccactttac
    11341 gtagcgggac cagaaagggg caacggtgaa cgccgaacaa tccaacacgc aagcaaaccc
    11401 gcaggaaccg ccgcacaagg ccctgatacc cgaggtcaag gcgcgctcgc cgcaggaagc
    11461 agcgaagctg ctcgccgagc acccggcgcc ggtaatcgcc gccacgctgc tcgagattaa
    11521 cccaggtata gcgcaggaca ttctctccga gctgtcgggc gggctgatgg acgcagtcct
    11581 gcatgcggtc gcccccgaga tggcgttgca gtggcagcgc aaccagacct atccgcagaa
    11641 cagcgtcggg cgcctgatgg agccggccta cgcggtattc caccccggca tgacggtcgc
    11701 ccagaccgtg gaacggttgc ggccgctgat caaggtcgcg ttcatcacct acggctacgt
    11761 gatcgacgag agcggcaagc tcggcggcct gatcaccatg cgcgacctgc tgttcgccga
    11821 cgacgatgcg cggctggagt ccttgatgct gcgcgacgtg ttcaccctca ggccggagac
    11881 gccgctcaac gaggcgatga agctcgtgct cgaccgccat tacccggttt acccggtatg
    11941 cgacggtaac ggcgtgctga tcggactggt gcgcgggcag gcgatgttcg aggagcaggc
    12001 gatcgagatc acggcgcagt atggttctct ggtgggcgtc gagaaggagg aacgtcttgc
    12061 cacgtccctg aaacaaagct tcaagttccg ccatccgtgg ctgcagttga acctcctcac
    12121 cgccttcgtc gcgggggcgg tggtcgccct gttccaggac acggtggacc ggctggtgat
    12181 cctggcggta ttcctgcccg tgctcgcggg tcagtccggc aacaccggct gccaggcgct
    12241 ggcggtcacg ctgcgcgggc tcaccctggg cgaactcaag accgggaagg aacgaaacct
    12301 agtcgcaaaa gaggcttggc ttggcttcta caacggcgcg ctgaccggcc tcgtcgccgg
    12361 gctcggcatg ctggcgatgg cgatctacca gggcaatccc aacgccatcc tgctggcggt
    12421 cgtggtgtgg atggcactgg tcggcgcctg cgtcacgagc ggcgtttgcg gcgcgatgat
    12481 cccgctgact ttgaagcgct tcggcctcga ccccgccacc gcttccagca tcttcctcac
    12541 caccgcgacc gatgtcgtca gcatgggcat gctgctcggc ctcgccgccc tgctcatccg
    12601 gtaatgcgtg aatggtaagt agtgaatagt gagcagacgc agcgcgcggg ttgcttcctc
    12661 gactcaccgc tcactaccca cgcctttcag gcaaggaagg cgttaaccgc ttccacaaaa
    12721 gcttccggac gctcaatttg cgggcaatgg ccgcagccgg cgatctcgag gaatctcgcg
    12781 ccggctattt tcgccgcgag ctcgcgcgcg agtacgggcg gcgtggtctg atcgagggcc
    12841 ccaaccatca ccagcgccgg gttgcggagc ttgccgagca cgggcgtgaa gtccagtctg
    12901 gcaagcgcca cgcacgcctg ctgaaagcac gccggatcgg cctcccgttc caggacgcgc
    12961 ttgcgttcgg ccacgatctg cggattggcg gcgatgaaag cctcggggaa catgcggcgg
    13021 atcgcgacgt cgagcgcgcc ggccattccc tcctgcgcga ctttcgccgc gaggttgcgc
    13081 agaggctgct tggccggttc cgggaacgtg gcaagcgcat ccgcgacgat cagccggtcg
    13141 aacttcgcgc cgtggcgtgc cgccagcgcg attgaaacga atcccccaaa gccattaccc
    13201 aggacgtccg tctggggcgg taatcgcatc gcggcgagca tcgctgctat gcgatcggcg
    13261 tagtcctcga cggcagcccc agcggggctc gaggcgccgt agccgggcaa attgacgagc
    13321 gtcaggcggc gcgatttaga caacgccgga acgacccgat cgaatgccga gcggtcggtg
    13381 agcagcgaat gcagcaggac gagatcgcgg cccgagccga tctgcacgat gtcgagatcg
    13441 gccccgttaa ccgtgacgat ggtcatggcg ggatgcgacc cggagtgacc actaacttac
    13501 aatccctctt ccctatgaaa aggggggtag cgatcaggcg gccactcttg ctttggtctt
    13561 ggctttggcc tgcgctgcct tggcgtgcat ggccgcataa gccgcgtgcg ccgcggcctc
    13621 cgcatcgccc acgtgcaccg gcaaccccat gtcggagagc acggaaccca gagcggcgag
    13681 gcacagcatc acgttgtcgg gacggcacga gtagcccatg atgccgaagc gccagatctt
    13741 gcccgcgaga ggccccaggc ccgcgccgat ctcgaggttg aattcggtga gaagcgtcct
    13801 gcgcaccgcg gcctcctcca caccttcggg gcagagcacc gcgttcatct gcggcaactg
    13861 gtactcgggc ttcaccagga acttgagccc catcgcctcg agtccggcct tgagcgcggt
    13921 gtggtgccgc cggtggcgcg cccagctgtt ctccagccct tcctcgtgaa tcagcagcag
    13981 cgcctcgtgc aacgcgaaca acgcgttggt gggcgcggta tggtggtaag tgcggttggt
    14041 cgcgccccaa tagccgagca gcaggttcat atccatgaac caggaatgga tcttgtcctt
    14101 gcgcgccttc acgtattcga ccacgcgctc ggagaacgac acgggcgaca ggcacggcgt
    14161 gcaggagagg cacttctggc tcgccgagta aatggcgtct atgccccact cgtccaccag
    14221 caccggggtg ccgccgagcg aggtgacggc gtccacgatg gtgagcgcgt cgtagcggtg
    14281 cgcgattccg accagcgtct tcgcgtccga ctggactccc gtggaggtct cggcgtgaac
    14341 gaacgcgacc acgcgcgcgt cacggttctt cttcaacgcg tcctcgagct tgtccggatc
    14401 gatcggctcg ccccatttgt cgtccaccac caccggaacg ccgcccatgc gctcgacgtt
    14461 ctcgatcatg cgcccgccga acacgccgtt gcggcacacg atcaccttgt cgcccggcgc
    14521 gaccaggttg acgaagcagt actccatgcc gaccgagccg gggcccgaga tcgggaaggt
    14581 gagcgggttc ttcgtctgat agacgtagcg cagcagcgcc ttcagctcct ccatcatctc
    14641 gacgaagacc ggatcgagat agccgatcgc cggctggctc atggtggtga gcacgcgcgg
    14701 gtggatctcg gtcggccccg gtcccatgag cgttctctgt ggcggatgga aggaggtgat
    14761 gcgcttgacg ggggcgtaat cgctcatgac gtctttccct gtaagaggcg aaccgaacag
    14821 gctctcgccg tccggccgcg atttaaatcg gaggggcttg cgggtgttgg cttcgttcag
    14881 gacctcgact gcggtgatct ggccggagct gagcgcctcc acttccatcg cccagcgcgc
    14941 cggcacgtag ccggacttca cccagttgct gaccactgcc cggtcaagcc gcaactggcg
    15001 cgcgacctcc gcttgggtgc caaacagggc gacgaggcgt tccgcgcagt tcatagcgcg
    15061 gaagattatc atgacaatat tattttgaca aattaggagg taaatatata cttgacagga
    15121 ctaaatcaat atgttagcat gtctccacag taggtaagga gacgaaaatg cttaaagagt
    15181 tcaacaacct aaacgagctt gaagcggcat tccccacaga ggaatcctgc atcgagcatt
    15241 acgcggagat tcgctggccg cgtggtgttg cttgcccaca ctgcggagtg atgaacgttt
    15301 acgagcttgc gaggaccggc tactacaagt gtgccgagtc gcgctgcggc aagaagttta
    15361 gctggcgcta cggctcaatc ttcgcggatg ccaagatcga gctgcggacc tggttccggg
    15421 ccatcttctt gatgacctcg cacaagaagg gtatttcgtc catgcagctt gcgcgggatc
    15481 tgggtatcac gcagaaaagc gcgtggcaca tgctccaccg tgtccgcgag gcaacaatga
    15541 ccgaagagtt taggcggccc ctgttgaccg gcacgattca ggcggacgag actttaatcg
    15601 gcggtaagga gcaattcaaa cacgccaaca agcgcacgcc gtttactgag gggcgcggct
    15661 ccatgcggac caagactatg gtattcggca tggttcaacc tggtggcgaa ttgcggatgc
    15721 aaaagcttcc agttatgtcg aggagggcaa tcgagcgcat cgtcaagaaa aacgtacgcc
    15781 ttggttccac ggttcacacc gatgatgcca cgcattatct atggatgaat gctcactatg
    15841 agcattcttt agtcaaacac cgcatgggcg aatacgtcgc caaggatggc accacgacca
    15901 acgcgatcga gggcgcgttc tcgcacttca agcggaccgt gaccggcgtt tatcacaagg
    15961 tcagtgacga gcagatcgac cgctacctgg gcatgttttc gtggcggtgg acccggcgta
    16021 agatggccga gggggagcgt gttaacgcgc tgctcaagtt cacaccgggc catcaattga
    16081 gctatcggaa actaactcgg aaggatgagg tgcaatgaac gtgttatggg ccgcttgtga
    16141 tagctccact taaaacccgg ttaaaaccgg gttttgtggt aaaattagct gatggttaaa
    16201 aggaaaaatg gccggccgaa aaacgaagag gcgaccgcta cgattgaggt aacggggacg
    16261 gagccgaagc tacttgccta tctagaagat ttgcggaaac ttcaggggtt tgggaattca
    16321 atatcagcaa tcgcccgcgg ttttatttgg aaagaagtga accgccttat agaggcgaaa
    16381 cgtctgaaac agaggtagaa actaatatgc tcatgacaga ccggcagatt gccgaggccc
    16441 gcactaaggg cgacgtcaag attgacccgt ttgaagataa gcagttgcaa cctgcgagtt
    16501 acgatttgcg cgtcggtgaa caaggcatta caactacttc aaagaaaaaa atcaacataa
    16561 aagctgatgg atttctggtt atacacccag gagattttgc tgtggtgttg gtacacgaag
    16621 aaattcgtct gggccctcaa tatgctgcgc ggtttggcct acggtcgaag tacgctcgta
    16681 ggggacttgt tgctactacc ggattgcagg ttgatcctgg atattatggc aatctgattg
    16741 tcggtatcac taacctcacg ccgaggccga tcacgctgcc gtacaaggac gatttattgt
    16801 ccatcgaatt tcatcggttg gaagaaccag ccagcaaacc atataacggc ccttaccaag
    16861 gacgaaaaga attggggcca gaagaaatcg agttcattat tgaaagcgag ggcatggcgc
    16921 tgtcagaagt gcttactaca ttgcgatcac tcagcgctaa tgtcggagtg ctgacgaacg
    16981 atgtgacagc acttactaac gagatgaagt attataagtg ggtggtgcca gccctcttag
    17041 ctatcggcat gacaataatc ggcgggatag tggcaataaa gtaaaacact cgcttctaat
    17101 gaatgagaag ccattccacc tatctatccc gttcgatgaa gcgcttgaac gtttagcgcg
    17161 tgtatcgaaa tcggttctac cggcaaaaaa aaagaaaaag cccgactcgc gccgggcttc
    17221 ccctacgaaa gggccagtta aatcacgtta ataatggttg gcgtatactc agcatccgat
    17281 tacctcctgg gcgggacagt ccccccacct tggggtgccg cagcagcagc acgggattag
    17341 cggcgtaagc cgcgaccgag gccggtagtg ttcacgcatt accggcctca cttttttcag
    17401 tgacggagat caagttgcgc tccgacatac tgaaaaatcc ctctttcaat ccgcctccag
    17461 tcgttcgact tgaacagcac gttaccgatg gcggtgcccg gcacgtccgt cccgaggtca
    17521 atgccgtcag cttccagttg atcaacgact gcttgaagcg taattgtctg gttggcggct
    17581 tgtctggcgg cttcgtgcgc gtattgcttg attaaaacgc tccgctcctc cttgggaatc
    17641 actctttttt gcggcgggct aatcttagta ctggtgggcg ttgcgtcttg ctcgcccttc
    17701 tgcaacatca ccatgttgga gaggacgctg cgctcacggt tcagctccgt caactcggca
    17761 cgtttcgcct caatctgctt ggcgatgtcc ttctcgacaa atttgcgggc ttgctcgatc
    17821 ttgccttcga gcaggagctt catcactttt tcggaattcg gagaaacttt actcatggcg
    17881 gcctcttgaa attcagaggc attttacctc tgtacgcaga ggtacaccag agggcttaga
    17941 ggcgcggtct agaccgctgt gaacttaccg cattatgaaa cgatttgtcc cgtcaggtat
    18001 atcattgcca attaggattt cgcaattgct acccttaccc acataccata tatctattta
    18061 aaatcaatcg cttaatttat agcgccgagg cgaaatcctc agtcgatccg taagccgatg
    18121 cgccggatca gggtgccgag gcgctcgtgc tcctcgcgga ggttcttctc gtattgctct
    18181 ggcgccgggc cgaccggctc cataccaatc gcggccatgc gcgcgaggac gtcgggggcc
    18241 cggaccactt cgttcaccgc ctggctgcgc cgcttgacga cttcgcggtc gtgcctcgac
    18301 accaaatgaa attaagtcac acgtctgcta ggac
//
LOCUS       DBNGWZ010000390         1610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2187061, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000390 DBNGWZ010000000
VERSION     DBNGWZ010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2187061"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1610
                     /locus_tag="AC16CX_11505"
     CDS             <1..>1610
                     /locus_tag="AC16CX_11505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013172.1"
                     /translation="SGVQEATLFSESGRILAFAGNERVGLMPEPPGAAALRQMRRDRA
                     YSAIEAIPDKGLYLRALVPVLPIAGEPRALQLLHPVPAQLARDAEAVQSGYREYQELT
                     LSRRGLKRLYGLTLTLTLLLALLSAVALAFVLSDRLSAPLGELVEGTRAVASGDFSQR
                     AAVASRDEIGVLIQSFNSMTLQLAEARAQAERHQAALVQAKAYLESLLANLSAGVLAF
                     DEELSLRAANRSAGAILGIDFTPLVGAPTARWPATAPGVAELGREISAAFERAGGSEW
                     EQQVERPLRDRTQLLLLRGTRLPQGAEGGCVVVFDDITQLAEAQRTAAWAEVARRLAH
                     EIRNPLTPIQLSAERLQVKLSGKLADADAEVLARSTQTIVNQVAALKNMVDAFSQYAR
                     TPEPSMRELDLNVITREVLTLYESSLGSSVRLDLDGTLPPVVGDAAQLRQVIHNLLQN
                     SQDALTETVEPRIIIASGVEGGMVRLTVTDNGCGFPEHVIQRAFEPYVTTKPKGTGLG
                     LAIVKKIVEEHGGTAAIANIAPRGARVT"
BASE COUNT          250 a          549 c          572 g          239 t
ORIGIN      
        1 tccggcgtgc aggaggcgac gctgttcagc gagagcggca ggatcctggc cttcgccggc
       61 aacgagcgcg tggggctgat gccggagccg ccgggcgccg cggcactgcg gcagatgcgg
      121 cgcgaccggg cctacagcgc gatcgaggca attcccgaca agggccttta cctgcgcgcg
      181 ctggtaccgg tgctgcccat cgccggggaa ccgcgcgcgc tccagttgct gcacccggtg
      241 ccggcgcaac tcgcgcggga cgccgaggcg gtgcagtcgg ggtaccgcga ataccaggag
      301 ctgaccctgt cgcggagggg gctcaagcgt ctctacgggt tgacgctgac gctgacgctg
      361 ctgctcgctc tgctctcggc ggtcgcgctt gcgttcgtgc tctcggaccg cttgtcggcg
      421 ccgcttggcg aactggtgga gggcacgcgc gcggtggcga gcggcgactt cagccagcgc
      481 gccgcggtcg cctctcgcga cgagattggc gtgctcatcc agtcgttcaa cagcatgacc
      541 ctgcaacttg ccgaggcacg cgcgcaggcc gagcgccacc aggcggcgct cgtccaggcc
      601 aaggcctatc tcgagagcct gctcgctaac ctttccgccg gcgtgctggc gtttgacgaa
      661 gagctcagcc tccgtgctgc aaaccgcagc gcgggcgcca tactcggaat cgatttcacc
      721 ccgctcgtcg gggcgccgac ggcgcgctgg ccggcaacgg ctcccggcgt cgcagagctg
      781 ggacgggaga tttcggcggc tttcgagcgc gccggcggaa gcgagtggga gcagcaggtc
      841 gagcgccccc tgcgcgacag gacccagttg ctgctgctgc gcggcacgcg cctgccgcaa
      901 ggagcggaag gcgggtgcgt cgtggtgttc gacgacatca cgcagctcgc cgaagcgcag
      961 cgtacagcgg cgtgggccga ggtggcgcgg cgcctcgcgc acgagatccg caacccgctc
     1021 acgcccatcc agctctccgc agagcggctg caggtgaagc tctcgggcaa gctcgcggac
     1081 gccgatgccg aggtgctcgc gcgctcgacg cagaccatcg tcaaccaggt tgcggccctc
     1141 aagaacatgg tggacgcctt cagccagtac gctcgcacgc ccgagccctc gatgcgcgag
     1201 ctcgacctca acgtgatcac gcgcgaggtg ctcacgctct acgagtcctc actgggttcc
     1261 agcgtgcggc tcgatctcga tgggacgctg ccgccggtgg tgggcgacgc ggcgcagctg
     1321 cggcaggtga tccataacct gctgcagaat tcgcaggatg cgctcaccga gaccgtggag
     1381 ccgcgcatca tcatcgcgag cggcgtcgaa ggcggcatgg tgcggctcac ggtcaccgac
     1441 aacggctgcg ggtttcccga acacgtgatt cagcgcgcgt tcgagcctta tgtcaccacc
     1501 aagcccaagg gcaccgggtt ggggctggcg atcgtcaaga agatcgtcga ggagcacggc
     1561 ggcacggcag ccatcgcgaa tatcgcgccg cgcggcgcgc gcgtcaccat
//
LOCUS       DBNGWZ010000391        13130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1557823, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000391 DBNGWZ010000000
VERSION     DBNGWZ010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1557823"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="AC16CX_11510"
     CDS             complement(<1..702)
                     /locus_tag="AC16CX_11510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013173.1"
                     /translation="MDRRKKRQLCGREPQAYERHVVPRLAAPLAMRLLEQLPLRAGQR
                     LLDVACGTGIVGRLAAPRVAPSGSVAGVDLDENMLAVARTRAADEGVSIDWKQADAGE
                     LPFADASFDIVVCQQGLQFFPDRVRAVREMRRVVSDGGIVALAVTGAPTAYNTALAQA
                     LARRGHATAARRSLAPFVLADPLMIRTIVSDAGFSAIDLRTEMIMRRVEPTQEWLLQE
                     SAGMPYGGVIVGMDDA"
     gene            complement(827..1930)
                     /locus_tag="AC16CX_11515"
     CDS             complement(827..1930)
                     /locus_tag="AC16CX_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013174.1"
                     /translation="MIGATQQIRFCTTSDGVRIAYAITGSGPPLVWAATRFTHIEFDQ
                     ASPLWRHWLAELSRGRTLVRYDERGCGLSDWNAEDLSFDAWVRDLEAVVDAAGIERFA
                     LIGILKGGVVAVAYAARHPERVSHLVLYGACARGKLARAATPKEREEEEVILKLVELG
                     WGEGNEAFRHLFAKEFIPDGMPEHHRSIEELFRLTTSPANAARISREYSMVDVRKLAA
                     KVRCPTLVMHSRGSLRHSLEEGRQLARLITDVRFVPLESRNHLLIEDEPAWGRFVAEL
                     RAFLPDAAPAVRTGALEGSFASLSARERDVLGLIAEGLDNRQIADRLELSEKTVRNHI
                     NSIFAKLDVPTRAQAIVRARDAGFGRRGAGTPH"
     gene            complement(1942..3105)
                     /locus_tag="AC16CX_11520"
     CDS             complement(1942..3105)
                     /locus_tag="AC16CX_11520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3013175.1"
                     /translation="MRKYALGYEATSSLEVIAVAEKVQAAVRTGPSKTEFREFPMPDI
                     PEDSALLKMEVAGICGTDVKLYAHPPTKSPVIMGHENIGTIAKAGREFTRRKGFREGD
                     LVFVEHYVMCGKCEWCHLGQYRHCENTDWRKNPDAIRYGYTSAEKAPHLWGGFAQYVY
                     LPWNAVVHHVPKGVTPELAGLVTPMANGVEWSLFDGGVGYNSTVLIQGPGQQGLSQTV
                     ICRQAGASLIIVTGTSKDRARMEVAKTLGADYVIDVSKEDPLARIMEITGGKGVDVAL
                     DCTAGAGTTPILLGVDALKRKGGTIVVQGEMKEFPNFPLEKVTVKFITIKSARGHSYK
                     ACELALEQLASKRFPLEKVTTHRFGLKDVDLAIRSVGGQGVPDVIHASLMPWK"
     gene            3235..3840
                     /locus_tag="AC16CX_11525"
     CDS             3235..3840
                     /locus_tag="AC16CX_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR3013176.1"
                     /translation="MKRLSVVTLLFAAALIVASCATMSPDSGWTTLIDGEKGLENFNR
                     IGDANWRAEDGAIVADKGKGGFLVSKNSYKDFQIRAEFWAGLNANSGIYMRCANPAVI
                     TDKSCYEANIYDERADPSFGTGAIQHITKASPMLKAGGKWSTYEITVKGTQITLVLNG
                     VQTATAQHSQFAQGPIALQFGSHGKEPGGVIKFRKVQIKGL"
     gene            4007..4921
                     /locus_tag="AC16CX_11530"
     CDS             4007..4921
                     /locus_tag="AC16CX_11530"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013177.1"
                     /translation="MSTVETRTKPRLAIMNAVHDLPVLVARDKGFFKDEGLDIEFVTT
                     PGMAQVTTSHSVKFDSVFDRPLDSVYNEGGIDQYRMCEWGIMKRAVEAECSGLRRRKI
                     VALGASMSKFAIAVARDSRIYEPEMLKNKEIAVTPNNGSHFTTLKMMEGFLKPEHVKT
                     TTAGSMPKRIEALKQGKVAAVTLMEPWISVAQKMGLRILIESHSTRSEAASDDLDGKT
                     LAAMFRAQARAVKEIEKDPTPYVHYFVRETGGLLEPHEFQAWRLLHAAPQPYTRERWE
                     DTYNWSVKWNMVVPGATYENIVDNRAWQ"
     gene            complement(5107..5970)
                     /locus_tag="AC16CX_11535"
     CDS             complement(5107..5970)
                     /locus_tag="AC16CX_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013178.1"
                     /translation="MPIAVIDGITTRYEVIGSGPPLLMYAPGGFDATVEKWTTLGVYA
                     RIKLLDHLPKKYTCIVFDRRECGQSGGRVEHITWAHYVAQGRGLLDHLNIKRAHLMGG
                     CMGCCPVMAFGVAYPEATLSMVLWWPVGGAKYRIRGHQRFAEHLAYVQQNGLAQVISL
                     VSKDGKPFGADPRGGPWASVIRNDRAFAESYAKLDVEQYKLVVSGMARSLLDRDTAPG
                     AEPEDLLRLNIPALVVPGRDESHATSAARYLEECLPRSEYWDVLPEAQTEDTAPARVL
                     QFLDSVSARAR"
     gene            complement(6073..6804)
                     /locus_tag="AC16CX_11540"
     CDS             complement(6073..6804)
                     /locus_tag="AC16CX_11540"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3013179.1"
                     /translation="MEKVTIRLLRQMKVAGRKIVGVVAWDYQIAQIAERAGVDFVSVG
                     DSVGVNLWGQLTETEVTMDEMLVVCKAVRRGVKRALVSCDIPFGPVHEGTGAAMSAAI
                     RLVKEGGADLVKVHGVPDTVRAIVRAGIPVFAEFHGKQVPAEKLVEEAKRLEDAGASL
                     LDFRDSGPVAGSAVVKAVSIPVIGGLGGGPWLDGRVRLAHTAVGYGAKWLDSKTETYG
                     NTARLCLEAFTALVNDVRAGRQIKG"
     gene            complement(6945..7802)
                     /locus_tag="AC16CX_11545"
     CDS             complement(6945..7802)
                     /locus_tag="AC16CX_11545"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3013180.1"
                     /translation="MPLPRLNGIIRALESGQHAFTTFMPPDAEAAIALQASKYDGVVF
                     EGEHNGWDIRVLRDCLQYLLNRAQIAKTGSVAPAVTPTVRIPPNGNEMNQFFAKQALD
                     LGYYGIIWPHVSTVEEAYNAVAACRYPRLKGKPLYEPAGIRGDGPTQAVRYWGITQQE
                     YYERADVWPLNPQGEIFVVIMIEDTTGIANLDDILKNVPGIGAVLIGEGDLSQELGYP
                     RQYEHKAVLDAMAQIVATCKKHNVVVGHPHVEAANAERIIKEGYRYLMCAAPRSFATL
                     EKARALAGR"
     gene            complement(7905..8942)
                     /locus_tag="AC16CX_11550"
     CDS             complement(7905..8942)
                     /locus_tag="AC16CX_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013181.1"
                     /translation="MIIDIHGHYTTEPQALHAFRDKQLAGLADAARRPASTDLGISDE
                     VLVKSVEPQLRFQNERGSDLTIFSPRASGMAHHVGTEATSIEWTRISNDLIHRICKLL
                     PDNFVGVGQLPQFPGASPKNCIPELERIVKELGFVGVNLNPDASGGNWTGPPLTDKYW
                     YPLYEKLCELEVPAMIHVSSSCNPNFHATGAHYINGDTTAFMQLIQGNLFKDFPTLRL
                     VIPHGGGAVPYHWGRYRGLAQDMKRPPLAEHLLKNVFFDTCVYHLPGIELLTKVIPVD
                     NVLFASEMVGAVKGIDPETGHYYDDTKRYIDQLKGLSEKDFYKIFEGNARRVYPRLDA
                     LLKKRGHAGKA"
     gene            complement(9037..10035)
                     /locus_tag="AC16CX_11555"
     CDS             complement(9037..10035)
                     /locus_tag="AC16CX_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3013182.1"
                     /translation="MAKAKLRLTLSIWDYDRTRALADGSVRPDGIELDVLELPVEETF
                     FRMARYREFDVAEMSMSSYTVSLHRDPQPFIAIPIFPSRFFRHSCIFISTKSGIREPK
                     DLIGRRIGTPEYQMTAPVWIRGILSDDYGVKPDSVEYWTGGEEETRRDEKIKLDLPPR
                     FKVRPIGPDKTLSAMIAAGEIDALHTARAPSTFYSKPDAVKRLFPDFVAVEREYYRRT
                     RIFPIMHVVAIRREVYEKNRWIAMSLHKAFVEAQRRVYEGFAQTAAHKAMLPWQNAHV
                     EDAVSEFGDDWWPYGFHGNRHVLDTFLRYHHEQGLSKRRLQPEEMFAPETLEAFKI"
     gene            complement(10244..11533)
                     /locus_tag="AC16CX_11560"
     CDS             complement(10244..11533)
                     /locus_tag="AC16CX_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HIR3013183.1"
                     /translation="MAITREQNEQLTRTGAGTLIGDLFRRYWIPALLAEEIAERDGPP
                     VRVQLLGEKLVAFRDTEGRIGMIDEFCAHRGVSLWFGRNEECGLRCPYHGWKYDVNGQ
                     CVDLPSEPEETGMRQRIKLKSYPCIEHGGVIWTYMGPPGLEPEPPALEWTLVPAANRF
                     VSKRLQECNYLQAIEGGIDSSHVSFLHSGALNSDPLFREGKRANIYNLGDRMPIFEVV
                     EYPGGLLIGARRRAENDRYYWRITPFIMPWNTLIPPRAGHPIGGHAWVPIDDENCWAW
                     SINYHPNRALKAAELNAMKEGKGIHVKYVPGTFIPLANKSNDYLMDRSTQKSGANYSG
                     IEGIAMQDASLQESMGPLQDRTQENLCSTDNGIIMARRMLIKAAKDNREGKPPPGLTA
                     DSQRVRSCSIELPKNVKFKDGAWDGLFRPLNTDPVTV"
     gene            complement(11651..11980)
                     /locus_tag="AC16CX_11565"
     CDS             complement(11651..11980)
                     /locus_tag="AC16CX_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HIR3013184.1"
                     /translation="MTDRKLVPLCRASDVAPGCALRAEAGGCVYAVFNVEGTIYVTQD
                     ECTHGPGSLSEGYVLDDEVECPFHQGRFHIPTGRATCAPATEPLKLWKVQIVDGKVCI
                     NPAEHEG"
     gene            12217..>13130
                     /locus_tag="AC16CX_11570"
     CDS             12217..>13130
                     /locus_tag="AC16CX_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HIR3013185.1"
                     /translation="MRTQIAIVGAGPAGLLLSHLLHLQGIRSVVLENRSRQYIEERIR
                     AGLLEQGTVDLLVESGVGERLKRESLVHHGIQLRFGGRGHRIDLSGLTGGRTVTIYAQ
                     HEVIKDLVKARLDAGGEILFEVSDVSVHDVTGATPRVRFRRDGVAHDLECDFIAGCDG
                     FHGICRDSIPAGALTVHDQHYPFAWLGVLAETPPVSPELIYTHHERGFALFTMRTTRI
                     SRLYLQCAPDEDIANWPDDRIWQELRTRLGTGGGVRLQDGPMLQKGITAMRGFVVEPM
                     QYGRLFLAGDAAHIVPPTGAKGLNLAVA"
BASE COUNT         2338 a         4249 c         4101 g         2442 t
ORIGIN      
        1 cgcgtcatcc attcccacga tgacaccgcc gtacggcatg ccggcgcttt cctggagcag
       61 ccattcctgc gtcggctcga cgcggcgcat gatcatctcg gtacgcagat cgatcgcgct
      121 gaatccggcg tcgctaacga tcgtacgaat catcaatgga tccgcgagaa cgaagggcgc
      181 gaggctgcgc ctcgcggccg ttgcatggcc gcgccgggca agcgcctggg caagcgcggt
      241 gttgtaggcg gtaggcgcac ccgtgacagc cagcgcgacg atgccgccgt ctgagactac
      301 gcgccgcatt tcgcgaaccg cacgcacccg gtccggaaag aactgcagac cctgctggca
      361 gacgacgatg tcgaagctcg catcggcgaa cggcaattcg ccggcgtcgg cctgtttcca
      421 gtcgatggac acgccttcgt ccgccgcgcg agtgcgcgcg acggcgagca tgttctcatc
      481 gaggtcgacg ccggctacgc tgccggaagg cgcgacccgc ggcgccgcga gtcggcctac
      541 gatgccggtg ccacaggcga catcgagcag gcgctgacct gcgcgcagtg ggagctgctc
      601 gagcaggcgc atggcgagcg gcgcggccag acgggggacg acatggcgct cataggcctg
      661 gggctccctg ccgcagagct gccgtttctt gcgtcgatcc actgttgccc gcgctgttgt
      721 cggggttcgg tccatgcaat cagtgtgcag gactcaacct cgcttgtctt gaggcgggtg
      781 acctagcgag cgtacgcgat ccgcaccgct atctgggact agcggcctaa tgcggcgtac
      841 ccgcgccgcg gcggccgaag cccgcgtcgc gcgcacgcac gatggcctgc gcgcgagtgg
      901 gcacgtcgag cttcgcgaag atgctgttga tgtgattcct gaccgtcttc tccgacagtt
      961 ccagtcgatc ggcgatctgc cggttgtcca acccttcggc gataagcccg agaacgtccc
     1021 gctcgcgcgc cgagagcgag gcgaagctgc cctcaagcgc ccccgtccgg accgcgggcg
     1081 ctgcgtcggg taggaacgcg cgcagctcgg ccacgaaccg gccccaggcc ggttcatcct
     1141 cgatcaggag gtggttgcgg ctttcgagcg gaacgaagcg cacatccgta ataagcctgg
     1201 caagctggcg accctcttcc aaagagtgtc gcagacttcc acgagagtgc atgacgagcg
     1261 ttggacatcg aaccttcgca gccagcttgc ggacgtcgac catgctgtac tcgcgcgaaa
     1321 tccgcgccgc attcgccggc gaagtcgtaa gacggaacag ttcttcgatc gagcgatgat
     1381 gctccggcat cccgtcgggg atgaactcct tggcgaaaag gtggcggaac gcttcgtttc
     1441 cctcgcccca tccaagctcg acgagcttga ggatgacttc ctcttcttcg cgctctttcg
     1501 gcgtcgcggc gcgcgccagc ttgccgcgtg cgcacgcacc gtacaatacg aggtggctga
     1561 cccgctcggg atgccgtgct gcatacgcaa ctgcgactac tccgcctttc aaaataccga
     1621 tcaacgcgaa gcgctcgatg cctgcggcat cgacgacggc ttcgagatcg cgaacccacg
     1681 cgtcaaagga gaggtcctcc gcgttccagt cggacagtcc gcagccgcgc tcgtcgtagc
     1741 gcacgagcgt gcggccgcgc gaaagctcgg cgagccaatg gcgccatagc gggctggctt
     1801 gatcgaactc gatgtgggta aaccgcgtcg cggcccaaac gagcggcggt ccgctgccgg
     1861 ttatcgcata cgcgatccga acgccgtcgc tcgtcgtgca gaaacggatt tgctgcgttg
     1921 cacctatcac actatgcctg tttacttcca cggcataagg gacgcgtgga tgacgtccgg
     1981 cacgccctgc ccgcccaccg accggatggc aaggtccacg tctttcagcc cgaatcggtg
     2041 agttgtcacc ttttccagcg ggaagcgttt tgacgcaagc tgctccaggg cgagctcaca
     2101 tgccttgtag ctatggccgc gcgcgctctt gatggtgatg aatttcaccg tgaccttttc
     2161 gagcggaaaa ttcgggaact ccttcatctc gccctggacc acgatcgtcc cgcctttgcg
     2221 cttcaacgca tccacgccga gcagaatcgg tgtagttccg gcgcccgccg tgcagtcgag
     2281 agcgacgtcc acgcccttgc cgccggtgat ctccatgatg cgcgcgagtg ggtcttcctt
     2341 gctcacgtca atcacgtagt cggcgccgag tgtcttggcg acctccatgc gcgcgcggtc
     2401 ctttgaagtg cccgtgacga tgatgaggga ggcgcccgcc tgcctgcaga tcacggtctg
     2461 ggacagtccc tgctggcccg ggccctggat gagcaccgtg gagttatagc ccacgccgcc
     2521 gtcgaacagg gaccattcga cgccgttcgc catgggcgtc accagccccg ccaattccgg
     2581 cgtcacgccc ttcggaacgt ggtgcacgac ggcgttccag ggaaggtaaa cgtactgggc
     2641 gaagccgccc cacagatggg gcgccttctc ggccgaggtg tagccgtagc ggatggcatc
     2701 ggggttcttg cgccagtccg tgttttcaca gtggcggtac tggcccagat ggcaccactc
     2761 gcatttgccg cacatgacgt agtgctcgac gaataccagg tcgccctccc taaagccctt
     2821 gcgccgggtg aactcgcggc ccgccttggc gatggtgccg atgttctcgt ggcccatgat
     2881 caccgggctc ttggtcggcg gatgcgcata caatttgacg tcggtgccgc agatcccggc
     2941 gacctccatc ttcagaagcg cgctgtcctc cgggatatcc ggcatcggga actcgcggaa
     3001 ctcggtcttg ctcgggccgg tccttacggc cgcctgcact ttttccgcca cggcgatcac
     3061 ctccaatgac gacgttgctt catagccaag tgcatatttt ctcatgcatt gcgcccacca
     3121 tcccacccgt agacggacgc cggactcgag accggcaagc cgatgtcttg acggtcaggc
     3181 accgggtggc ccatcctttg tgcggtatcg tcatcaacta cgggggaggt cgtcatgaaa
     3241 cgactgtctg tcgtcacctt gctgtttgcc gccgcgctga tcgttgccag ctgcgcaacc
     3301 atgtcgccgg actcgggctg gaccacactg atcgacggcg agaagggcct ggagaacttc
     3361 aaccgcattg gcgacgccaa ctggcgggcg gaggacggcg ccatcgtggc cgacaagggc
     3421 aaggggggct tcctggtttc gaagaactcc tacaaggact ttcagatcag ggccgagttc
     3481 tgggccggtc tcaacgcaaa cagcggcatc tacatgcgat gcgccaatcc cgccgtcatc
     3541 accgacaaga gctgctacga ggcgaatatt tacgacgagc gcgccgaccc gagtttcggc
     3601 acgggagcca tccagcatat tacgaaagcg tcacctatgc ttaaggccgg cggcaaatgg
     3661 agcacctacg agatcacggt aaaaggcacg caaatcaccc tggtgctgaa cggcgtccag
     3721 accgctactg ctcagcacag ccagttcgcg cagggaccga tcgccctcca gtttggcagc
     3781 cacggcaaag agcccggcgg cgtcatcaag ttccgtaagg tgcagatcaa ggggctgtaa
     3841 cccgcatttg atcgacccgc cgtcttcggc ggtcccctca ccccaaccct ctccccacct
     3901 tcgcggggag agggagtttt cttgtccgct tccggactcc accgttgaca ggccaatgac
     3961 cgaagcggat cattttcagc cgtccgcttt cacaggagga atacctatgt ctaccgtgga
     4021 aaccaggacg aaaccccggc tcgcgatcat gaacgcggtc cacgacctgc cggtgctggt
     4081 cgctcgcgac aaggggttct tcaaggacga gggcctggac atcgagttcg tgaccacgcc
     4141 ggggatggcc caggtcacga cctcgcactc cgtcaagttt gattccgtct tcgaccgccc
     4201 gctcgactcg gtttacaacg agggcggcat cgaccagtac cgcatgtgcg agtggggcat
     4261 catgaagcgc gccgtcgagg cggaatgctc ggggctccgc cggcggaaga tcgtggcact
     4321 gggcgcatcg atgtcgaagt tcgccatcgc cgttgcccgc gactccagga tctacgagcc
     4381 cgagatgctc aagaacaagg agatcgcggt gacgccgaac aacggctcgc acttcaccac
     4441 gctcaagatg atggaagggt tcctgaagcc ggagcacgtc aagacgacca ccgcgggatc
     4501 gatgcccaag cgcatcgagg cgttgaagca gggcaaggtc gcggcggtga ccctgatgga
     4561 gccctggatc agcgtggcgc agaagatggg cctgcgcatc ctgatcgagt cgcactccac
     4621 ccgcagcgag gcggccagcg atgatctcga cggcaagacg ctcgcggcga tgttccgggc
     4681 ccaggcgcgg gcggtgaagg aaatcgaaaa ggaccccacg ccgtacgtcc actacttcgt
     4741 ccgcgagacc ggcgggctcc tggagcccca cgagttccag gcctggcgcc tgctgcacgc
     4801 cgcgccgcag ccctacacgc gcgagcgctg ggaagacacc tacaattggt cggtcaagtg
     4861 gaacatggtg gtgcccggcg ccacctacga gaacatcgtc gacaatcgag cgtggcagta
     4921 gcgcgccctg ttccgttcgt ccttgttgag gacgacaagc agacaaagcc gccgcagggc
     4981 ggctttgttt tttcacgctg gcgcgtgaat ggccgggggg cggtcccggc gccggtgact
     5041 ttcttattgc tcgcccataa tggagaaagt cactaggccc cggcagggga ctatatgggg
     5101 gaggcgctac cgcgcccgtg cggacacgct atccaagaac tggagcaccc gcgccggggc
     5161 ggtgtcctcg gtctgggcct ctggcagaac atcccaatac tcggatcgcg gcaggcattc
     5221 ttccagatac ctcgccgccg aagtcgcgtg cgactcatcc cggcccggaa cgacgagagc
     5281 gggaatattc aggcgcagca aatcctcggg ttcagccccg ggagcggtat cgcgatccag
     5341 cagagaacgg gccattcctg acacgacgag cttgtactgc tccacgtcca gcttcgcata
     5401 ggactcggca aacgcgcggt cattccggat gaccgaggcc cagggtccgc cccgggggtc
     5461 ggctccgaaa ggcttgccgt ctttactgac cagcgagatc acctgcgcaa gaccgttctg
     5521 ctgcacgtac gccagatgtt ccgcgaagcg ctgatgcccc cggatgcggt acttggcgcc
     5581 gcccacgggc caccagagca ccatgctcaa cgtcgcctcc gggtacgcta cgccgaaagc
     5641 catgaccggg cagcacccca tgcagccgcc catgaggtgc gcgcgcttga tgttcaggtg
     5701 gtccagcaac cccctgccct gcgccacgta gtgagcccag gtgatgtgtt ctactcggcc
     5761 gcccgattgg ccgcattcgc gccggtcgaa gacgatgcag gtgtatttct tcggcagatg
     5821 gtccaggagt ttgatccggg cgtagacgcc gagcgttgtc catttttcca cggtcgcgtc
     5881 gaatccaccg ggcgcgtaca tcagcaaggg cggcccggag ccgatcacct cgtagcgtgt
     5941 cgtgatgccg tcgatgaccg caattggcat gatgcgtgat gggtgatgag tgatgggtga
     6001 tgggattccc gcactgcggg agccttatca cttattacct atcacttatc accctgttcg
     6061 aagtaaatgc catcaaccct tgatctgacg gccggcacgc acgtcgttga ctagcgccgt
     6121 gaaagcttcg aggcacagcc tggcggtatt cccgtaggtt tcggtcttcg aatcgagcca
     6181 ctttgctcca tagccgaccg cagtgtgcgc gaggcgtacc cgcccgtcga gccatgggcc
     6241 cccaccgagc ccgccgatca caggaatgga gacggcctta acgaccgcgg agcctgccac
     6301 cggcccggag tccctgaagt cgagaagcga tgcgccggca tcttccagcc gctttgcctc
     6361 ttccacgagc ttctctgcgg ggacctgctt cccatggaac tcggcaaaga ccgggatccc
     6421 cgcgcgaacg atcgcacgca ccgtgtcggg aacgccatgg accttcacca ggtcggctcc
     6481 tccctccttg acaaggcgta tggcagcgct catcgcggcc ccggtgccct catgcacggg
     6541 accaaaagga atgtcgcagc tgacgagcgc gcgctttacg ccacgccgca ccgccttgca
     6601 caccacgagc atctcatcca tcgtgacttc agtctccgtg agctggcccc ataaattgac
     6661 gcccaccgag tcccccacgg aaacgaaatc cacacccgcg cgttccgcga tctgcgcgat
     6721 ctggtagtcc cacgcaacca cgccgacgat cttgcggccg gcgactttca tttgccggag
     6781 gagacggatg gttaccttct ccacggcgtc gactcccaac tgactgcgcc ggccaaaagg
     6841 atgagggatg agggatgagg gatgaccccc gcagtgcgga aacaaaatac caggccagcc
     6901 ttcatccttc cgccttcatc cttcaggcgc agccgcgctg gttactagcg gcccgccagg
     6961 gcacgggcct tctccagggt cgcgaaactg cgcggcgcgg cgcacatcag gtagcggtaa
     7021 ccttccttga tgatgcgctc tgcattcgcg gcctccacgt gcggatggcc gacgaccacg
     7081 ttgtgcttct tgcaggtggc gacaatctgc gccatcgcgt cgagcacggc cttatgctcg
     7141 tactggcgcg gatagccgag ctcctggctc aaatcgcctt cgccgatcag cacggcgccg
     7201 atgcccggca cgtttttcag gatgtcgtcc aggttggcga ttcccgtggt gtcctcgatc
     7261 atgatcacga cgaagatctc tccctgcggg ttgagcggcc acacgtcggc gcgctcgtag
     7321 tattcctgct gcgtgatgcc ccagtaacgc accgcctgcg tcggcccatc gccgcgaatg
     7381 cccgccggct cgtagagcgg cttccctttc aggcgcgggt atcggcaggc ggcgaccgcg
     7441 ttgtaggcct cctcgaccgt gctgacgtgc ggccagatga tgccgtagta gccgagatcg
     7501 agcgcctgct tggcgaaaaa ctggttcatt tcgttgccgt tgggaggaat gcgcaccgtg
     7561 ggcgtcaccg ccggcgccac cgagccggtc ttggcgatct gcgcgcggtt cagcaggtac
     7621 tgcaggcaat ccctcagcac gcgtatgtcc cagccgttat gctcgccctc gaacacgaca
     7681 ccgtcgtatt tcgacgcctg cagggcgatc gccgcctcgg cgtccggcgg catgaaggtc
     7741 gtgaaggcgt gttggccgga ttcgagcgcg cggatgatgc cgttcaagcg gggcaaaggc
     7801 atgcgggttc ctcccgatga tgcgtgataa gtgataggtg acaggtgata ggagtttagc
     7861 aagcccgcta cgcgggagcc ttatcactta tcactcatca cttatcacgc ctttccggca
     7921 tgtccgcgct tcttgagcag cgcatcgagc cgggggtaga cacggcgagc attgccttcg
     7981 aagatcttgt agaagtcctt ctcgctcaag cccttcaact ggtcgatgta gcgcttggtg
     8041 tcgtcgtagt agtgcccggt ctccggatca atgcccttca ccgcgccgac catttccgag
     8101 gcgaacagga cgttgtccac ggggattacc ttggtgagca actcgatgcc cggcaggtga
     8161 tagacgcaag tgtcgaaaaa gacgttcttc agcaggtgct cggcgagcgg cggccgcttc
     8221 atgtcctggg caaggccgcg gtagcggccc cagtggtacg gcaccgcgcc acccccgtgc
     8281 gggatgacaa gcctgagcgt cgggaaatcc ttgaagagat tgccctggat gagctgcatg
     8341 aatgccgtcg tgtcgccgtt gatgtaatgt gcgcccgtgg cgtggaaatt cggattgcag
     8401 gacgaggaca cgtggatcat tgccggcact tccaactcgc acagcttctc gtagagcgga
     8461 taccagtact tgtcggtcag cggcggaccg gtccagttgc cgcccgaagc atcgggattc
     8521 aggttgacgc cgacgaaacc gagctccttc acgatgcgct cgagctcggg aatgcagttt
     8581 ttcggcgacg cgcccgggaa ctgcggcagc tggccgacac cgacgaaatt atcgggcaac
     8641 agcttgcaga tacggtggat gaggtcgttg gagatccggg tccattcgat gctggtcgcc
     8701 tcggtgccta cgtgatgggc catgccggag gcgcgcggcg agaagatcgt gagatcgctg
     8761 ccgcgctcgt tctggaacct gagctgcggc tcgacgctct tcaccagcac ctcgtcactg
     8821 atgccgaggt cggtcgaggc cggcctgcgc gcggcatcgg cgagtcccgc gagctgcttg
     8881 tcgcggaacg cgtggagcgc ctgcggctcg gtcgtgtaat ggccgtggat gtcgatgatc
     8941 atgagagttg gctttccgca atcagcgttc agctatcagc ttaatacaag tcccgggttg
     9001 gctgaaagct gatggctgac ggctaacagc tggcgttcag atcttgaagg cttccagggt
     9061 ctccggcgca aacatctcct cgggctggag acggcgcttg gacaatccct gctcgtgatg
     9121 gtagcgcagg aaggtgtcga gcacgtggcg gttgccgtga aagccgtacg gccaccaatc
     9181 gtcgccgaac tcgctcaccg cgtcctcgac gtgcgcgttc tgccagggca gcatcgcttt
     9241 gtgcgccgca gtctgggcga aaccctcgta gacgcggcgt tgtgcctcga cgaaggcctt
     9301 gtggagcgac attgcaatcc agcggttctt ctcgtagacc tcgcgccgga tcgccaccac
     9361 gtgcatgatc gggaaaatgc gggtgcgccg gtaatattcg cgctcgaccg cgacaaagtc
     9421 cggaaacagg cgtttcaccg cgtcgggctt cgagtagaag gtcgagggcg cgcgcgcggt
     9481 gtgcagcgcg tcgatctcgc ccgcggcgat catcgccgaa agcgtcttgt cgggcccgat
     9541 cggacgcacc ttgaacctgg gcgggaggtc gagcttgatc ttttcgtcgc gccgggtctc
     9601 ctcctcgccg ccagtccagt actcgacact gtcaggcttc acgccgtaat cgtcggacag
     9661 gatgccgcgg atccacaccg gcgccgtcat ctggtactcg ggcgtcccga tcctcctgcc
     9721 gatgagatcc ttcggctcgc ggatgccgct ctttgtggat atgaaaatgc aggaatggcg
     9781 gaagaaacgc gaagggaata tcgggatcgc gatgaacggc tgcggatcgc ggtggagcga
     9841 caccgtgtag gacgacatcg acatctccgc gacgtcgaac tcgcggtagc gcgccatccg
     9901 gaagaaggtt tcctcgaccg gcagctcgag cacgtccaac tcgatgccgt ccggccgcac
     9961 cgagccgtcg gcgagcgcgc gggtgcggtc gtagtcccag atggacaggg tcaggcgcag
    10021 cttggccttg gccacgataa gcctttatgg agctgacagc tgacggctga cgactgacgg
    10081 cggacggggc cggcccgaag gatgagggat gaaggatgag ggatgacccc cgcagtgcgg
    10141 gttttcaaca cggtcatcct tccgccttca tccttcatcc ttggtcgtga ccggtcaccg
    10201 gtcacagaac ccgagtcgag ccatgatcct cgacctaggg gctctagacc gtgacggggt
    10261 cggtattcag cgggcggaac agcccgtccc aggcgccgtc cttgaacttg acgttcttgg
    10321 gcagctcgat cgagcacgag cgcacgcgct gcgagtcggc cgtgaggccg ggcggcggct
    10381 tgccctcgcg gttgtccttc gccgccttga tgagcatccg acgcgccatg atgatgccgt
    10441 tgtcggtcga gcagagattc tcctgcgtgc ggtcctggag tggtcccatg ctctcctgca
    10501 gcgaagcgtc ctgcatcgcg atgccctcga tgccgctgta gttggcgccc gacttctgcg
    10561 tgctgcggtc catcaggtaa tcgttgctct tgttggcgag cgggatgaaa gtgccgggca
    10621 cgtacttgac gtgtatgccc ttcccctcct tcatcgcatt caactcggcc gccttcagcg
    10681 ctcggttcgg atggtagttg atggaccacg cccagcagtt ctcgtcgtcg atcggcaccc
    10741 aggcatgccc gccgatcgga tggcccgcgc gcggagggat cagcgtattc cagggcatga
    10801 tgaagggagt gatgcgccag taatagcggt cgttttcggc ccggcggcgc gcgccaatca
    10861 gcagccctcc cgggtactcg accacttcga agatcggcat gcgatcaccc aggttgtaga
    10921 tgttggcccg cttgccttcc ctgaacagtg gatcgctgtt cagcgcgccg ctgtgcagga
    10981 acgagacgtg gctcgaatcg atgccgccct ctatcgcctg cagatagttg cactcctgga
    11041 gccgcttgga tacgaagcgg ttcgcggccg gcaccagcgt ccattccagc gccggcggct
    11101 cgggctcgag ccccggcggc cccatgtagg tccagatcac gccgccgtgt tcgatgcagg
    11161 ggtaggactt cagcttgatg cgctggcgca tccccgtttc ctccggttcg gacggcagat
    11221 ccacgcattg tccgttcacg tcatacttcc agccgtggta cgggcagcgc aggccgcatt
    11281 cctcgttgcg cccgaaccac agcgagacac cgcggtgcgc gcagaattca tcgatcattc
    11341 cgatgcggcc ttccgtatcg cggaacgcca cgagtttttc tcccagcagc tgcacgcgga
    11401 cgggcggacc gtcccgctcg gcgatttcct cggcgagcaa cgccggaatc cagtagcggc
    11461 ggaaaaggtc gcctatcaag gtcccggcgc cagtgcgggt caactgctca ttctgctctc
    11521 tggtaattgc catgtttcaa gtcctctact ttccaaacga tctgagtggt gggtgacgag
    11581 tgtcgggtgt cgaggggtaa accccattca cgttttcctc actcgacact caatcctcga
    11641 cactcgcttc tcacccctcg tgttccgccg gattgatgca gaccttgccg tcgacgatct
    11701 gcactttcca cagcttcaac ggctcggtgg ccggcgcaca cgtcgcccgc ccggtcggaa
    11761 tgtgaaaccg tccctgatgg aacgggcact cgacttcgtc gtcgagcacg tagccctcgg
    11821 agagcgagcc ggggccatgg gtgcattcat cctgggtcac gtaaatggtg ccctcgacgt
    11881 tgaacacggc gtagacgcaa cccccggctt ccgcgcgtag cgcgcacccc ggagccacgt
    11941 ccgacgcgcg gcacagcggc accagtttcc ggtccgtcat agctgacgcg tattgtaccc
    12001 cgccaccgcg gatggacacc gttgttccgc atgggggaac cagccatctc ctcgccgtca
    12061 ttgcggcggc attgtaaacc cggagcgcgc gactttgttg accaggatca aggaatcttt
    12121 tctgcgcacg cgtccactcg cggggccgcc ccttccggat tatcattccg gcagtcgtta
    12181 ccataatcac aagtccgggc tcaccagcaa tactcgatgc ggacacaaat cgccatcgtc
    12241 ggggcggggc ccgccggttt actgctctcg cacctgctgc acctgcaggg catccgctcg
    12301 gtcgtgctcg aaaatcgcag ccgccagtac atcgaggagc ggattcgtgc gggcctcctc
    12361 gagcagggca cggtcgacct cctggtcgaa tccggcgtag gcgagcggct caagcgcgaa
    12421 agcctggtcc atcacggtat ccagttgcgt ttcggcgggc gcggacaccg catcgacctg
    12481 agcgggctca ccggcgggcg cacggtcacg atctacgcgc agcatgaagt catcaaggac
    12541 ctcgtcaagg cgcgcctcga cgcgggcggt gagatcctgt tcgaggtcag cgacgtcagc
    12601 gtgcacgatg tcaccggtgc gacgccgcgg gtccgcttcc gcagggacgg cgtcgcgcac
    12661 gatctggaat gcgacttcat cgccggctgc gacggctttc acggcatatg ccgggattcc
    12721 atccccgcgg gcgcgcttac cgtgcacgac cagcactatc ccttcgcgtg gctcggcgtg
    12781 ctggccgaga cgccgccggt atcgcccgag ctcatttaca cgcatcacga gaggggcttc
    12841 gcgctcttca ccatgcgcac cacgcggatt tcgcgcctgt acctccagtg cgcgccggac
    12901 gaggacatcg ccaactggcc cgatgaccgg atctggcagg agctgcgcac gcgcctcggc
    12961 accggtggcg gcgtgcggct gcaggacggg cccatgctgc agaagggaat caccgcgatg
    13021 cgcggcttcg tcgtcgagcc gatgcagtac ggcaggctgt tcctcgccgg cgacgccgcg
    13081 cacatcgtac ccccgaccgg cgcaaaggga ctcaacctgg ccgtcgccga
//
LOCUS       DBNGWZ010000392         4070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_673772, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000392 DBNGWZ010000000
VERSION     DBNGWZ010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_673772"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..2854
                     /locus_tag="AC16CX_11575"
     CDS             2..2854
                     /locus_tag="AC16CX_11575"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3013186.1"
                     /translation="MMKQMLGNSYLFGANAPFIEALYDAWLKDPGSVEPRWRDYFDEL
                     QRLDQGAADISHFDVQERFAQLASARRAAAPTVVINRPQLGEKQFAVLQLISAYRFQG
                     ARHADIDPLKRREKPSIPELDPAFYGLNDADMEMVFNTGTLVGPREMKLKDILRFLRD
                     TYCRTIGVEYMYITDMAHKRWVSERLESIRSTPSYDAAYKKHILERLTAAETLEKYLH
                     TRYVGQKRFSLEGGESLIPQLDNLLQQAGKAGVQELVIGMAHRGRINVLVNTMGKMPK
                     DLFAEFEGKHHDDLLAGDVKYHQGFSSNVMTPGGPMHVTLAFNPSHLEIVNPVVEGSV
                     RARQHRRSDKTGDQVLPVLIHGDAAFAGQGVNMETFEMAQTRGYGTGGTVHIIANNQI
                     GFTCSDLRDARSTLYCTDIAKMLEIPIFHVNGDDPEAVCLATEIALDYRMKFHKDVVV
                     DLVCYRRLGHNEQDEPMVTQPLMYKTIAKLPTTRKLYADRLEREGTVQPGEGDEMVKT
                     FRTALDGGHHTNKTILSDYRPPHEIDWSKYKDGKWNEHDDTRLPLEKLKRLGECITTV
                     PENFKLHPSVQRIVENRRLMAQGKLPLDWGMAEHLALATLLDEGYSVRLSGQDSGRGT
                     FFHRHAVLHDQNREKWDQGTYVPLQHVSDKQGDFVVIDSLLSEEAVLGFEYGYATSAP
                     HELVIWEAQFGDFANGAQVVIDQFIASGEAKWGRLCGLVLMLPHGYEGQGPEHSSARI
                     ERYMQLCADYNMQVCIPATPVQLYYLLRRQMIRPFRRPLILFMPKSMLRHKESVSPLE
                     EFAIDKFKPVNEDWESKDINPDNVKRVIFCSGKIYYDLRAERKARGSKDLPIARIAQL
                     YPFPHEDFQAQIERYRKASEIVWCQEEPRNQGAWHRIQHYLQRHMLPHQKLIYAGRDS
                     SASPAAGYKSLHDRQQKELVELALSVGVVSRADGVR"
     gene            2854..>4070
                     /gene="odhB"
                     /locus_tag="AC16CX_11580"
     CDS             2854..>4070
                     /gene="odhB"
                     /locus_tag="AC16CX_11580"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR3013187.1"
                     /translation="MLVEVKVPQLAESVAEATLLTWHKKPGEAVKRDENLVDIETDKV
                     VLETPAPESGVLVRIVKGDGGTVTSNEVIAQIDTEAKAAAAAPAAAKEEAKAGAKSPA
                     KATAAPAMPAAQKIAVEKHVDTAAIPGTGRGGRVTKEDVLQAVGGGVQPAAKTPAATS
                     VPQRAPAPAVDLTALGERPEQRVPMSRLRARVAERLVQSQHTAAILTTFNEVNMQPVM
                     ELRKKYQERFEKQHGIRLGFMSFFVKAAVHALKRYPIVNASVDGSDILYHGYYDIGIA
                     VGSPRGLVVPILRDADQLSLADIEKQIADFGKRAQEGKLTIEELTGGTFSISNGGVFG
                     SMLSTPIINPPQSAILGVHATRERPVAENGQVVIRPINYLALSYDHRIIDGREAVLSL
                     IAIKEALEDPARML"
BASE COUNT          802 a         1327 c         1334 g          607 t
ORIGIN      
        1 catgatgaag cagatgctcg gcaactcgta tctcttcggc gccaacgcgc cgttcatcga
       61 ggcgctctac gatgcctggc tcaaggaccc gggctcggtc gagccgcgct ggcgcgacta
      121 tttcgacgag ctgcagcgcc tcgaccaggg cgccgcggat atttcgcact tcgacgtcca
      181 ggagcgcttc gcgcagctgg ccagcgcgag gcgcgcggcc gcgccgacgg tggtgatcaa
      241 ccggccgcag ctcggcgaga agcagttcgc ggtgctgcag ctcatctcgg cctaccgctt
      301 ccagggcgcg cgccatgccg acatcgaccc tctcaagcgc cgcgagaaac cgagcattcc
      361 ggaactcgac cccgcgtttt acgggctgaa cgacgcggac atggaaatgg tgttcaacac
      421 cgggacgctg gtcgggccgc gggagatgaa gctcaaggac atcctgcggt tcctgaggga
      481 tacctactgc cggaccatcg gcgtcgagta catgtacatc acggacatgg cgcacaagcg
      541 gtgggtttcg gagcgcctgg aaagcatccg ttccacgccc agctacgatg ccgcttacaa
      601 gaaacacatc ctcgagcgcc tgaccgcggc cgaaacgctg gaaaagtacc tccatacccg
      661 ctacgtcggg cagaagcgct tctcgctgga agggggagag tcgctcatcc cgcagctcga
      721 caacctgctg cagcaggcgg gcaaggccgg cgtgcaggag ctggtgatcg gcatggccca
      781 ccgcgggcgc atcaatgtgc tggtcaacac catgggcaag atgcccaagg atctcttcgc
      841 cgagttcgag ggcaagcacc acgatgatct cctcgccggc gacgtcaagt accaccaggg
      901 cttctcctcc aacgtcatga caccgggcgg cccgatgcac gtgacgctgg cgttcaaccc
      961 ctcccatctc gaaatcgtca atcccgtcgt cgagggctcg gtgcgggcgc gccagcaccg
     1021 ccgcagcgac aagaccggcg accaggtgct gccggtcctc atacacggcg atgccgcgtt
     1081 tgcggggcag ggcgtcaaca tggaaacgtt cgagatggcg cagacgcggg gctatggcac
     1141 cggcggcacc gtgcacatca tcgccaacaa ccagatcgga ttcacctgtt ccgacctgcg
     1201 cgatgcgcgt tcgacgctct attgcacgga catcgccaag atgctcgaaa taccgatctt
     1261 tcacgtgaac ggcgacgatc cggaggcggt gtgcctggcc acggaaatcg cgctcgacta
     1321 ccgcatgaag ttccacaagg acgtggtggt ggacctggtg tgctaccggc gcctcgggca
     1381 caacgagcag gacgagccga tggtgaccca gccgctgatg tacaagacga tcgcgaagtt
     1441 gccgaccacg cgcaagctct acgccgaccg gctcgagcgc gagggcacag tccagccggg
     1501 cgagggcgac gagatggtga aaaccttccg caccgcgctc gacgggggcc accacaccaa
     1561 caaaaccatc ctttccgact acaggccgcc gcacgagatc gactggagca agtacaagga
     1621 cggcaaatgg aacgagcatg acgacacgcg gctgccgctc gaaaagctga agagactggg
     1681 cgagtgcatc accaccgttc ccgagaactt caagctgcac ccctcggtgc agcggatcgt
     1741 cgagaaccgg cggctgatgg cgcagggaaa gctcccgctc gactggggca tggccgagca
     1801 cctggcgctt gccacgctgc tcgatgaagg ctactcggtc cggctctcgg gccaggacag
     1861 cgggcgcggc acgttcttcc accgccacgc cgtgctgcac gaccagaacc gggagaaatg
     1921 ggatcagggt acctatgtgc cgctgcagca cgtgagcgac aagcagggcg acttcgtcgt
     1981 catcgactcg ctgctgtcgg aggaggcggt gctgggcttc gaatacggct acgcgacttc
     2041 cgcgccccac gagctggtga tctgggaggc gcagttcggc gatttcgcca acggcgcgca
     2101 ggtggtgatc gaccagttca tcgcctcggg tgaggccaag tggggacggc tgtgcgggct
     2161 ggtgctgatg ttgccgcacg gctacgaggg gcaggggccg gagcattcct ctgcgcgaat
     2221 cgagcgctac atgcagctct gcgccgacta caacatgcaa gtatgcatcc cggcgacgcc
     2281 ggtgcagctg tactatctgc tgcggcggca gatgatccgg ccgttccgca ggccgctcat
     2341 cctgttcatg ccgaagagca tgctgcggca caaggagtcg gtgtcgccgc tggaggagtt
     2401 cgccatcgac aagttcaagc cggtcaacga ggattgggag tcgaaggaca tcaaccccga
     2461 caacgtgaag cgcgtcatct tctgcagcgg caagatctat tacgacctgc gcgccgagcg
     2521 caaggcgcgc gggtcgaagg acctgccgat cgcgcgcatc gcccagctct acccgttccc
     2581 gcacgaggac ttccaggccc agatcgagcg ctaccggaag gcgagcgaga tcgtatggtg
     2641 ccaggaagag ccgcgcaacc agggcgcgtg gcaccgcatc cagcactacc tgcagcggca
     2701 catgctgccg caccagaagc tgatctatgc gggccgtgat tcatccgctt cgccggcggc
     2761 gggctacaag tctctgcacg accggcagca gaaggagctg gtggaactcg cgctctcggt
     2821 gggcgtcgtt tcgcgcgcgg acggggtgcg gtaatgctgg tcgaagtcaa ggtcccgcag
     2881 cttgcggaat cggtggcgga agccacgctc ctcacctggc acaagaagcc gggcgaggcc
     2941 gtcaagcgcg acgaaaacct ggtggacatc gagaccgaca aggtggtgct cgagacgccg
     3001 gcgccggaat ccggcgtgct cgtcaggatc gtcaagggcg acggcggaac ggtcacgagc
     3061 aacgaggtga ttgcgcagat cgacaccgaa gccaaggctg cggcggcggc gccagccgcg
     3121 gccaaagaag aagcgaaagc gggggcaaag tcccccgcaa aggcgactgc ggcgccggca
     3181 atgccggcag cccagaagat cgcggtggaa aaacacgtgg acaccgccgc gatccccgga
     3241 actggccgcg gcggccgcgt caccaaggaa gacgtgctgc aggcggtcgg gggcggggtc
     3301 cagcccgccg cgaagacccc ggccgccacg tccgttccgc aacgcgcgcc ggcgcctgcg
     3361 gtggatctca ccgcgctggg cgagcggccc gagcagcgcg tgccgatgtc gcggctgcgg
     3421 gcgcgcgtcg ccgagcgtct ggtgcagtcg cagcacacgg cggcgatcct caccacgttc
     3481 aacgaagtca acatgcagcc ggtgatggag ctgcgcaaga agtaccagga acggttcgag
     3541 aaacagcacg gcatccggct cggcttcatg tcgttcttcg tcaaggcggc ggtgcacgcg
     3601 ctcaagcgct atcccatcgt caacgcctcc gtggacggca gcgacatcct ctaccacggc
     3661 tattacgaca tcggcatcgc ggtcggcagc ccgcgcggct tggtggtgcc gatactgcgc
     3721 gatgccgacc agctgtccct cgcggacatc gagaagcaga tcgcggattt cggcaagcgc
     3781 gcgcaggaag gcaagctcac gatcgaagag cttaccggcg gcacgttctc gatctccaac
     3841 ggtggcgtct tcggctcgat gctgtccact cccatcatca accccccgca gagcgcgatc
     3901 ctcggcgtac acgccacgcg cgagcgcccc gtcgccgaga acggccaggt ggtgatccgg
     3961 ccgatcaact acctcgcgct gtcctacgac caccgcatca tcgacgggcg cgaggcggtg
     4021 ctctccctga tcgcgatcaa ggaagcgctg gaggatcccg cccgcatgct
//
LOCUS       DBNGWZ010000393         4650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2187664, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000393 DBNGWZ010000000
VERSION     DBNGWZ010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2187664"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..775)
                     /locus_tag="AC16CX_11585"
     CDS             complement(2..775)
                     /locus_tag="AC16CX_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HIR3013188.1"
                     /translation="MPSGWRMAAAALAAALLGGCASLRDAVTLPYVSSFSDERPGEEL
                     PRGWEPWTLSRFKKPTQYQLVAQDGRTVVKASAHASASGLIHPISVNPKSYPLLSWRW
                     KVTQLIARADNTRKQTEDSPVRVVVSFAGDHDKLSLNDRIFMDNIRLLTGQQLPYATL
                     MYIWENRAPKGTVIPNLHTSRIKMIVAESGRDKLGNWQEVTRNVYEDFKRAFGEEPGR
                     ITAIGIMTDTDNTGENVHAYYGDIVFRRVAPPRVTFASD"
     gene            complement(897..1502)
                     /locus_tag="AC16CX_11590"
     CDS             complement(897..1502)
                     /locus_tag="AC16CX_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013189.1"
                     /translation="MGDLIDALAAAQAALVADAPDALDQAASKLVFCHWVSGSAWRRY
                     LVELREFEMGVKIAAALADDVGPIAGELARRLAALPAIAVSRHEGASALACAICGKDA
                     VTFRTSAQGLQCSTLSPINAGPECRDEIAARLTALLDNGDGRAVLGLLTGPDGPGCDA
                     YCPECDRVYCKDHYAVETTWSGSWHESTSATCPLAHAREFE"
     gene            complement(1879..2931)
                     /locus_tag="AC16CX_11595"
     CDS             complement(1879..2931)
                     /locus_tag="AC16CX_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011205693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR3013190.1"
                     /translation="MAQTTRIPRILPTMGQPASERRHVAILAFDDCQLLDVAGPLQAF
                     ATASELAGPHAFPGYTVSVYSRGGGPVRTSSGLVLLTRPLRALRVDGRTTVMLPGGRG
                     VRDAASDRKLIARIQQANRRAERICSVCTGAFLLAATGLVDGRRVTTHWRSCEQLQEL
                     HPRLRVERDPIFVRDGRYWTSAGVTAGIDLALALIEADAGKALALETARHLVVFFKRP
                     GGQAQFSDLLAAQSRESARFAQLHAWIAAHLRGDLTVASLAERACMSPRNFQRVYKAT
                     MGATPARMIERIRVEAARRLIEDGAARVGAVAKAVGFGDDETMRRAFLRQIGVSPVHY
                     RRGFLSPQSGVRQPSP"
     gene            3014..3586
                     /locus_tag="AC16CX_11600"
     CDS             3014..3586
                     /locus_tag="AC16CX_11600"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR3013191.1"
                     /translation="MRAAIIVFDNMTTLDFVGFYDPLTRLKSMNFMPDLEWRICARQP
                     RVTDDRGLAVVADSVGEGLEAFDLVFVPGGFGTRPLQHDRSFVDWIGSAQRARLKISV
                     CTGAMLLGAAGFLRGRRATTHPSALKELEPYCAAVVAQRIVDEGEVITAGGVTASIDL
                     GLHVVERLGGADTRARIARQMDYPYRPDAR"
     gene            complement(3589..4107)
                     /locus_tag="AC16CX_11605"
     CDS             complement(3589..4107)
                     /locus_tag="AC16CX_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3013192.1"
                     /translation="MSKAAPLLLSLLVFPLLLAAAPADADILARVVSVHDGDTLTVLI
                     ERRQMKVRLSDIDAPELGQPFGTRSRQSLAELCFGKPASLDVRGRDRYQRTIARVTCA
                     GTDANAEQVRRGYAWTFTRYARADSPLYALQREARAARRGLWNDPSPVPPWDWRRYGQ
                     HSSRTNGIRPGS"
     gene            complement(4325..>4650)
                     /locus_tag="AC16CX_11610"
     CDS             complement(4325..>4650)
                     /locus_tag="AC16CX_11610"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR3013193.1"
                     /translation="LKQTLLDLSQTATINDSLKLGRAVLNELGGVNTLHKPQVEQAGF
                     AVLKAPDIPSILVETAFISNPEEERKLRNDAYQEKMAEAVFVGIKRYFAKNPPLARER
                     LVQNP"
BASE COUNT          771 a         1501 c         1574 g          804 t
ORIGIN      
        1 atcagtcgct tgcgaaggtg acgcggggcg gagcgacgcg gcgaaaaacg atgtcgccgt
       61 agtaggcgtg gacgttctcg ccggtgttgt cggtgtcggt catgatgccg atcgcggtga
      121 tgcggccggg ctcctccccg aacgcgcgct tgaaatcctc gtacacgttg cgcgtcactt
      181 cctgccagtt ccccagcttg tcgcgcccgc tctcggcgac aatcatcttg atgcgcgagg
      241 tgtgcaggtt ggggatgacc gtgcccttgg gcgcgcggtt ttcccagatg tacatcaagg
      301 tcgcgtaggg caattgctgg ccggtgagga ggcgaatgtt gtccatgaag atccggtcat
      361 tgagggacag cttgtcgtga tcccccgcaa aactcacgac cacgcgcacc ggggaatcct
      421 cggtctgctt ccgggtgttg tccgccctgg cgatgagctg cgtcaccttc cagcgccagg
      481 agagcagcgg ataggacttg gggttgacgc tgatcgggtg gatcagcccg gaggccgagg
      541 catgcgcgct cgccttgacg acggtcctgc cgtcctgggc gaccagctgg tactgggtcg
      601 gcttcttgaa cctggacagc gtccacggct cccagccccg cggcagctct tcgcccgggc
      661 gttcgtccga gaagctgctg acgtagggca gcgtcaccgc gtcgcgcagc gacgcgcaac
      721 cgccgagcag cgccgcggcg agcgccgccg cggccatcct ccatccactg ggcatcttat
      781 cgttgttttg caagcgtttt gccggatgtt accccaaata tcgtgcgggc gaccaattcg
      841 ggtcgttccc gaccgggcgg caagcggcct gggcggtcat tcagggcatt gggagttcac
      901 tcgaactccc tggcatgtgc cagtggacag gtcgcgcttg tggactcgtg ccagcttccg
      961 ctccatgtgg tctcgacggc gtagtggtcc ttgcagtaga cgcgatcgca ctcagggcaa
     1021 taagcgtcgc accccggacc gtcgggcccc gtcagcagcc ccaacacggc gcggccgtcg
     1081 ccgttgtcga gcagcgcggt caggcgcgcg gcgatctcgt cgcgacattc gggacccgcg
     1141 ttgatcggcg agagcgtgct gcactggagc ccttgtgctg aggtgcggaa cgttacggcg
     1201 tccttgccgc agatggcgca tgcgagggcg ctggcgcctt catgcctgga aaccgcaatc
     1261 gcgggaagcg ctgccaagcg gcgggcgagc tcgcccgcaa tcggcccaac gtcgtccgcc
     1321 agcgcggcgg cgattttcac gcccatctcg aattccctga gctcgaccag atagcgccgc
     1381 cacgcgctgc cgctgaccca gtggcagaag acgagcttgc ttgccgcctg gtcgagcgca
     1441 tcgggcgcgt cggccacgag cgccgcctgc gcggcggcga gcgcgtcgat cagatcaccc
     1501 acgcgctcct ccaccgccgc ttttacttcg gcggaaagcg gctccgaccc tgataccacc
     1561 ctgctccgga gcgtggtcag cgccggtgcg ggcgggttag ccgcaaggtc cacgcgggca
     1621 ggagggcgga gcggagttat ggacgcgcgt cgcgggcgct gtggcaccgc tgcggacttc
     1681 cgccatcggt ttctgatcgt ttccacgacg gcggccagga ccaggaggcc catgccagcc
     1741 cagaaaataa tcttgatcgc gtctaacaag atatcaaaca cgtgatgggt gatgggtgat
     1801 gagtgatggg catcccgcgc tgcgggggtc gtcccccatc cctcatcctt catccttcgt
     1861 cgtattcccc gtcatcattt atgggctggg ttgacgcacc ccgctttgcg gcgacagaaa
     1921 cccgcgccgg tagtgaacgg gcgatacgcc gatttggcgc agaaatgcgc gccgcatggt
     1981 ctcatcgtcg ccaaagccga cggctttcgc caccgcgccg acgcgggccg cgccatcctc
     2041 gatcagccgg cgcgccgcct cgacgcgaat gcgctcgatc atgcgggccg gcgtggcgcc
     2101 catggtcgct ttgtagactc gttggaaatt gcgcgggctc atgcacgcgc gctcggccag
     2161 gctcgcgaca gtcaggtcgc cgcgcaaatg ggccgcaatc cacgcgtgca actgggcgaa
     2221 tcgcgcgctc tcgcgcgact gcgccgcgag caggtcgctg aactgggcct ggcctcccgg
     2281 gcgcttgaag aacacgacga ggtgccgggc cgtctccagc gccagcgcct ttccggcatc
     2341 ggcttcgatc agcgccagcg cgaggtcgat gcccgcggtc acgccggccg aagtccagta
     2401 gcgcccgtcc cggacgaaga tcggatcgcg ctccacccgc aaccggggat gcagttcctg
     2461 aagctgctcg caggaccgcc agtgggtcgt gacgcggcgt ccatccacga gcccggtggc
     2521 ggcgaggaga aacgcgccgg tgcagacgga gcatatgcgc tcggcgcggc ggttcgcttg
     2581 ttgaatccgc gcaatcagct tccggtcgga cgccgcatcg cgcacgccgc gcccgcccgg
     2641 gagcatgacc gtggtgcggc cgtccacccg cagggcgcgc agcgggcgcg tcagaagcac
     2701 gagcccggaa ctggtgcgaa ccgggccgcc gccgcgcgag tacaccgaga ccgtatagcc
     2761 gggaaaggcg tgcgggccgg caagctccga ggcggtggcg aacgcctgga gcggcccggc
     2821 cacgtccagc agctggcaat cgtcgaaggc gaggatggca acgtggcgtc tctctgacgc
     2881 gggctggccc atggttggca gaatacgagg gattcttgtc gtttgcgcca tccacggtat
     2941 tgctaagatg gccgcgtgtc aatcccgccg gggccggacg cgccgccagc ctctcgcgaa
     3001 aggagaccgc gccatgcgcg ccgcaatcat cgtgttcgac aatatgacga cgctcgattt
     3061 cgtcggcttc tacgatccgt taacgcggct caagtcgatg aatttcatgc cggacctcga
     3121 atggcggatc tgcgcccgcc agccgcgggt caccgacgat cgcggcctgg cggtcgtcgc
     3181 ggattccgtc ggcgaagggc tcgaggcgtt cgacctggtt ttcgttccgg gcggattcgg
     3241 gacgcgcccg ctccagcacg accgttcatt cgtggactgg atcggctccg cgcagcgggc
     3301 aaggctcaaa atatccgttt gcacgggtgc catgctgctc ggcgccgcgg gcttcctgcg
     3361 cggccgccgc gcgacgaccc atccgagcgc gttgaaggag ctcgaaccct attgtgcggc
     3421 agtcgtggcg cagcgcatcg tcgacgaggg cgaggtgatc acggccggcg gcgtcacggc
     3481 ctcgatcgac ctcggcctgc acgtggtgga gcggctcggc ggcgccgata cgcgcgcccg
     3541 gatcgcgcgc cagatggact acccgtatcg cccggacgca cggtaaggtc acgagccggg
     3601 tcgaataccg tttgtccgcg aggagtgctg gccgtaccgc cgccagtccc acggcggcac
     3661 cggcgaaggg tcgttccaca ggccgcgacg cgcagcccgg gcttcacgct gcagggcata
     3721 taggggcgaa tcggcgcggg catagcgcgt gaacgtccac gcgtagccgc ggcgcacctg
     3781 ctcggcattg gcgtcggtgc cggcgcaggt gacccgggcg atggtccgct ggtagcggtc
     3841 ccggccgcgg acgtcgagcg aagccggttt tccgaaacag agctcggcga gggactgccg
     3901 cgagcgcgtg ccgaacggct ggcccagctc gggcgcgtcg atgtccgaca gccgcacctt
     3961 catctggcgg cgctcgatca acaccgtcag ggtatcgccg tcgtggaccg acaccacgcg
     4021 cgcgagaatg tccgcgtcag caggagcggc ggcgagaagg agagggaata cgagtaacga
     4081 aagtaggagg ggggccgcct tactcaagcg gatgcccaag tagagtaatg gaaccgccga
     4141 tgaacgcaga tgaacacaaa tcaatcaaaa ccgaagcgtt cattcacagt aatcctgatc
     4201 ggcgtctatc tgaggtcgga attgaaccgc agataatcac tgataaacgc tgatatataa
     4261 cagcaaccag aaagcaggtt atttataatt tttctgcgtt tatcggtgtt tatcggcggt
     4321 ttcattacgg attttggacg aggcgctcgc gcgcgagcgg cgggttcttc gcgaaatagc
     4381 gcttgatgcc gacgaacacc gcctccgcca tcttttcctg gtaggcgtcg ttccggagct
     4441 tgcgctcctc ctcgggattg ctgataaagg cggtctccac caggatcgag gggatgtcgg
     4501 gcgccttcag caccgcgaag ccagcctgct ccacctgcgg cttgtgcagg gtgttgacgc
     4561 cgcccagctc gttcagcacc gcgcggccga gtttcaggct gtcgttgatg gtcgcggtct
     4621 gcgagaggtc gagcagcgtc tgcttgagat
//
LOCUS       DBNGWZ010000394         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_299186, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000394 DBNGWZ010000000
VERSION     DBNGWZ010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_299186"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16CX_11615"
     CDS             <1..609
                     /locus_tag="AC16CX_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013194.1"
                     /translation="LGRRLRDYVGATWPEFDWNAAYHEFYESYRPRSGLSGRHGGVAL
                     EALGLCVTTAQAAVFYRALADCADEPALRVLAREAACDHAVYFDFFRALFERCKRIER
                     VGFAAAWRTAIATCRSARDDDVAAAFQPLGRNWNGTPIVPDLDYPEYRQRLAQLVRRR
                     AALGPVERLLFRPWLERSRAAPAPQLSSAPPGRWLPLAPQPA"
     gene            750..1316
                     /gene="msrA"
                     /locus_tag="AC16CX_11620"
     CDS             750..1316
                     /gene="msrA"
                     /locus_tag="AC16CX_11620"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR3013195.1"
                     /translation="MHLDPATAVPPSKETATLAGGCFWCLEAVYDGMKGVASVESGYM
                     GGRTPNPTYEQVCGGDTGHAEVVRITFDPAVVSFKELLEVFFVIHDPTTLNRQGNDSG
                     TQYRSAIFYLTPRQKADAEEVIARLNAAKLWNGPIVTEVTPASTFYMAEGYHQEYFAR
                     NPYQPYCQFVVAPKVAKFRKHFLEKLRK"
     gene            1737..2051
                     /locus_tag="AC16CX_11625"
     CDS             1737..2051
                     /locus_tag="AC16CX_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HIR3013196.1"
                     /translation="MAKKRKSTKKSKKVGKAKKSGRKPNPAFMKPMTPSSTLAAVIGG
                     SAMPRTEVTKKLWAYIKRNGLQDRVNKRMINADDKLRPVFGGRSQVSMFEMTRLVSNH
                     LH"
     gene            complement(2109..3239)
                     /locus_tag="AC16CX_11630"
     CDS             complement(2109..3239)
                     /locus_tag="AC16CX_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013197.1"
                     /translation="MSIRLIVFLTVLTHLSFAGCRVIISLTAIHYQASPFTVGVVMSL
                     LTVIPMLFAVRWGRYVDRIGVRGPMLAGVAAILAAMVLGWAVPSLETLFVVSPLAGSG
                     FFLYHIASGQAAAVIGRPQDRVRNFSLLALAYSASGFLGPTLAGFAIDGIGHDNAMLA
                     LSAGVLVNAAVLAAWKAEVPRHEAVAAQGGKRRVADLLRIPGFRLVFIVSGTLSMTWD
                     LFAFVTPIYGSQIGLSASTIGVMLGMFGAAVFVVRLALPLVAHRVSEWRMLISAMALS
                     GATLLAFPLVSGVPLLMALAFLLGVGLGGTQPLIMVLLYNKAPAGRGAEVLSVRTWLI
                     NLSQTSVPLTFGALGTALGMLPVFWAMGLSLLVAGFVAARGK"
BASE COUNT          655 a         1173 c         1134 g          505 t
ORIGIN      
        1 ctcggccgcc ggctgcgtga ttacgtcggg gcgacctggc ccgagttcga ctggaacgcc
       61 gcctatcacg agttctacga gagctatcgt ccgcgctccg ggctgtcagg ccggcacggc
      121 ggcgtcgcac tcgaagcgct tgggctgtgc gtgacgacgg cccaggccgc ggtgttctac
      181 cgcgcgctcg ccgactgcgc cgacgagccg gctttgcgcg tgctcgcgcg cgaggcagcg
      241 tgcgatcacg cggtttattt cgactttttc cgcgcgcttt tcgagcgctg caagcggatc
      301 gagcgcgtcg gctttgccgc cgcctggcgg acggcgattg cgacctgccg ctcggcgcgc
      361 gatgacgacg tggccgccgc gtttcagccg ctcggccgga actggaacgg cactccgatc
      421 gtgcccgacc tcgactatcc cgagtaccgg caacgcctgg cgcaactggt ccggcgccgc
      481 gccgcgctgg ggccggtcga acgcctgctg ttccggccgt ggctcgagcg cagccgcgcc
      541 gcgccggcgc cgcaattgtc ttccgccccg ccgggccggt ggctgccgct ggcgccgcaa
      601 ccggcgtgac ggtcgagtct tgcggaattg tcttgtcaat ccgttccgca accatcgacg
      661 atggcacggc gcgggcggcg agccgcccgc gccgtgaatc cgctacactc ggtgcaccgt
      721 atcaagggac tcttgatctg ataggagcga tgcatttgga tcccgccacc gcagtccccc
      781 ccagcaagga aaccgccacg ctcgccggcg gctgcttctg gtgcctcgag gcggtgtacg
      841 acggcatgaa gggagtggcc tccgtcgaat cgggctacat gggcgggcgc acgcccaacc
      901 cgacctacga gcaggtctgt ggcggcgaca cgggacacgc cgaagtcgtg cgcatcacct
      961 tcgatcccgc ggtggtgtcg ttcaaggagc tgctggaggt gttcttcgtg atccacgacc
     1021 cgaccacgtt gaaccgccag ggcaacgact cgggcaccca gtaccgttcg gcgatcttct
     1081 atctcacgcc gcggcagaaa gccgatgcgg aggaggtgat cgcgcgcctc aatgcggcga
     1141 agctgtggaa cggccctatc gtcaccgagg tcacgcccgc atccaccttc tacatggccg
     1201 agggctacca ccaggagtac ttcgcgcgca acccctacca gccgtactgc cagttcgtgg
     1261 tcgcgccgaa agtggccaag ttcaggaaac acttcctgga aaagctcagg aagtagcgcc
     1321 gccgcgcgga tgccgggtcg cccggccgcc catcgcgctg cgcaccgcat cccggcgtcc
     1381 gtatccgcct ggtgcgaagg gaactgccga gtcaaccggc ggggcccttc tcgctccaga
     1441 aacgcttgtc cagacttgaa cttctgcgga taagtccttg acttcatgct gcggcgggac
     1501 ggatacgcgc tacgggggcc ggtggctacg cgcgcggcaa ggcgtgcgct gcatggttgc
     1561 gacagccttt gacacagctc aaaagtacgt ggcgaaacgc cccgcgcaca gcgaaaaacc
     1621 cctaaaaacc ccttgcaagg ttggctggac attggcatcc tctactcgcc gaattcacgg
     1681 ttagacggcg ttaccaacct ccgcgctgtt tcagcgcaat caacgggaga aaatcgatgg
     1741 ccaagaaaag gaaaagcacc aagaagtcca agaaggtcgg gaaggcgaag aagagcggcc
     1801 gcaagccgaa ccccgccttc atgaaaccga tgaccccgag ctccacgctc gcggcggtga
     1861 tcggcggcag cgcgatgccg cgcaccgagg ttaccaagaa actgtgggcc tacatcaagc
     1921 gcaacggcct gcaggaccgc gtcaacaagc gcatgatcaa cgccgacgac aagctgcgcc
     1981 cggtgttcgg cggcaggtca caggtgtcca tgttcgagat gacccggctg gtgagcaatc
     2041 acttgcattg accggacttc aagtcgaaac cggggctgca ttcatgcagc cccggttttt
     2101 tttcgccgtc acttcccgcg cgcggcgaca aaaccggcga cgaggagcga caggcccatc
     2161 gcccagaaca ccggcagcat gccgagcgcg gtcccgagcg cgccgaaggt gagcggcacg
     2221 ctggtctggc tcaggttgat gagccaggtg cgcacgctca gcacctcggc gccgcgcccc
     2281 gccggcgctt tgttgtagag cagcaccatg atcaacggct gggtgcctcc gagcccgacg
     2341 ccgagcagga aggcaagcgc catcagcagt ggcacgccgc tcaccagggg gaacgcgagc
     2401 agcgtcgcgc ccgaaagcgc catcgccgag atcagcatgc gccactcgct cacgcggtgc
     2461 gcgaccagcg gcagcgcgag cctcacgacg aacacggcgg cgccgaacat gccgagcatg
     2521 acgccgatgg tggacgcgga gagcccgatc tgcgaaccgt agatcggggt gacgaaggca
     2581 aacaggtccc aggtcatcga cagcgtgccg ctcacgatga acaccagccg gaagcccggg
     2641 atgcgcagca ggtcggcgac gcgtcgttta cccccttggg cggcgaccgc ctcgtgccgg
     2701 gggacctcgg ccttccacgc tgccagcacc gcggcgttca ccagcacgcc cgccgagagc
     2761 gcgagcatcg cgttgtcgtg gccgatgccg tcgatggcaa agccggccag cgtcggcccg
     2821 agaaaaccgg aggccgagta ggcgagcgcg agcaggctga aattcctgac gcggtcctgc
     2881 ggccgcccga tcaccgccgc cgcctggccg gacgcgatgt ggtagaggaa gaagcccgat
     2941 cccgcaagcg ggctcaccac gaacagcgtc tccaggctcg gaacggccca cccgagcacc
     3001 atcgccgcga ggatcgccgc gacgccggcc agcatcggcc cgcgcacgcc gatgcggtcc
     3061 acgtagcgcc cccagcgcac cgcaaacagc atcggaatca ccgtgagcag cgacatcacc
     3121 acgccgacgg tgaaagggga ggcctggtag tggatcgccg tgagcgaaat gatgacgcgg
     3181 cagccggcga acgacaggtg cgtgaggacg gtgagaaaga cgatgaggcg tatgctcaaa
     3241 gcgcgcgcac ccgtaaaaat ttgattttac ccgcgcagcg tcgtgccttt ccgcaatgtg
     3301 gattgccgat gagcgcgctc aaacttttcg ccatgcgacc agcgcgacgc cgcagcaggt
     3361 gacggcgatg ccggcgaccg aaagcgcgct cggcacgact ccgaacatcg ccagctccag
     3421 caccaccgcg aagatcggcg tgagatagag gaggctggtg acgcggg
//
LOCUS       DBNGWZ010000395        11334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_47967, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000395 DBNGWZ010000000
VERSION     DBNGWZ010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_47967"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC16CX_11635"
     CDS             complement(<1..657)
                     /locus_tag="AC16CX_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013198.1"
                     /translation="MSLTTVAHDWTDLTHTVLRALTIEEGRSGFGQIAEDLATRAVGT
                     IMAYSLKWQTVAAKLLLEAWSDAQREQGDIVGQLAATGISDLFDIPVSPAEAVPGRGA
                     AGAGLRNKVGQAVLDGLLSKIRPTGALGPDDGLRAAEAVLGQTVGLGIEGWLEGLYFG
                     GPLGDYFNGAIADLDDVVAASAGLGSAARRAIGPVLRVLVAEPLTRWLNAQYRPRIYN
                     E"
     gene            complement(696..1466)
                     /locus_tag="AC16CX_11640"
     CDS             complement(696..1466)
                     /locus_tag="AC16CX_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013199.1"
                     /translation="MGAVLGWIWNAIVYVAEKAAELAIFLWEAAKQAAMQIWHVVKEA
                     ARYAAEFARWVRNGVDYLWTRYVKPIAQALWTAAEKVSAALKRVFGPVIEILVKLQKA
                     LTWLWTHVVSPILDAIGMARRVLQLLGKLGLEWALKADAWLADLERRIVQGFSKLTGW
                     LNEVLDFLWQITTPAGLLNPKWLFGSMAPYVSTIGRWIVQNGVSTFEDHEKDKLLREY
                     PPNTHADLDRQLATGEIRRRPVADVTHAIVLRIFRDKV"
     gene            complement(1469..1678)
                     /locus_tag="AC16CX_11645"
     CDS             complement(1469..1678)
                     /locus_tag="AC16CX_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013200.1"
                     /translation="MTEAKIVRVDETFRLSPRGETQRVVRVSFFVGEQGPFSKDYTPA
                     EFIPERVKRDLADEARKFEELGISG"
     gene            complement(1678..2223)
                     /locus_tag="AC16CX_11650"
     CDS             complement(1678..2223)
                     /locus_tag="AC16CX_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013201.1"
                     /translation="MDPFEVSPFDSRRGGTGLVSAAEALAAELMSLAPIIEAHASRAD
                     QARHSGIAELASGADESERHMRELHAYDTEEEADAAGGTLTGGDFTQLVNGRYPVHPG
                     EAEQPPPGYYLTDHVTGPAHRMRGDTERMRGKAPWRDDDERGGGDRGAGRREPAEEPP
                     PPPPAETPPAEPPTRERNGGR"
     gene            complement(2249..2491)
                     /locus_tag="AC16CX_11655"
     CDS             complement(2249..2491)
                     /locus_tag="AC16CX_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013202.1"
                     /translation="MSDEEYQRTQESLVSLAALALLLPDLSRFLERIVEAHALGPFVD
                     PTRYRRGMANLAKIETLARAAHAFQREARRQAGQPG"
     gene            complement(2528..2845)
                     /locus_tag="AC16CX_11660"
     CDS             complement(2528..2845)
                     /locus_tag="AC16CX_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013203.1"
                     /translation="MSATPPVGRRQLSRGGRRGFVFSIRLTAEELAELAKAAYRERPW
                     YTTSPALGPWIKYAALERARDGAAKLGKAPGDRPGTTHDQAGDPKKGTTGRRRRVVPR
                     RGK"
     gene            complement(2842..3192)
                     /locus_tag="AC16CX_11665"
     CDS             complement(2842..3192)
                     /locus_tag="AC16CX_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013204.1"
                     /translation="MIRSAAVLVLVAALAAPAAAGDAAWIFAGNGLNAGTTLWGRAQG
                     ARELNPLLQDRGALVAWELGMAAGTTLACRELRRRDHPRAAKWFSRVGFALNVVVAAH
                     NVRQGVLARQRRGQ"
     gene            complement(3224..3463)
                     /locus_tag="AC16CX_11670"
     CDS             complement(3224..3463)
                     /locus_tag="AC16CX_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013205.1"
                     /translation="MIGAIVGAAARILLPRAAARLVLWVGRVRLAKRILGWGDEDPET
                     DRDEAREDLDYAVLWREAQDELEDEEDREDLTGPL"
     gene            complement(3487..4368)
                     /locus_tag="AC16CX_11675"
     CDS             complement(3487..4368)
                     /locus_tag="AC16CX_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013206.1"
                     /translation="MWTAEEYAQMAQALREAMGRPDPVAFDRFRAAFPGALYVSRDDE
                     LELRTYGSVAGMTAVARVRMLSPGGDVVPNTWRLATSANGTAGTLRVQVGEGFLLALT
                     VENEGGAPMRGQHYAAVRVFHGGPITPERVQVLVEGYITTETLLAWPAPTSAAGPDPR
                     RALVVTAVANPAAGAEISITIPTARRARLQLLYFDLVTSVTVANRSLELIIDDGVDIL
                     WREVAFDGQPASTTRHYMVAPIGFEHAVRANDAVITVPAEFWMRGGWRIRTITALLQA
                     ADQFSAIRLVTEEHLDV"
     gene            complement(4372..4659)
                     /locus_tag="AC16CX_11680"
     CDS             complement(4372..4659)
                     /locus_tag="AC16CX_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013207.1"
                     /translation="MSARRFRCNSCGYVYVEATPGHSHRLHRCVPAVNPGYDPDPGSP
                     TYDPRDYLPIDNPRDENPEYDLDFDPDHPRPRRPTRQRAEGKGRTLVSEEE"
     gene            complement(4656..5513)
                     /locus_tag="AC16CX_11685"
     CDS             complement(4656..5513)
                     /locus_tag="AC16CX_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013208.1"
                     /translation="MTHALGNPELARVIFARRNVQPPTTLYVGRDDYLTVTEIHSELA
                     QDPPLVLVRLLTPLGVVQHMTYRTPAFPASSGFSKTRFPLREGQLLGVEVRAAAGGAK
                     RGDLYAIVELTHSLGPIDQEHAILCADYVTLYQPVGWPQGMVRSAEEGPGHERTFTQP
                     APFAGQPIQLSIPSLLRWRIKGIRASLTTTAVAGNRLVSLAVIALGGQQVTIALSTVE
                     HPPSGTRAYHFSDRATSNVQGLIATELLPIPLNVWWNANATLQVRVSGAGGDQFSASD
                     WFVEEAISA"
     gene            complement(5613..6416)
                     /locus_tag="AC16CX_11690"
     CDS             complement(5613..6416)
                     /locus_tag="AC16CX_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013209.1"
                     /translation="MGLGLELLTGFTTGATAAMTGVQMAAGNSLTVRNSRPDADIRLL
                     QMWADVQEATVLHRLRSPKMHDNVQGLRFLNAASTVQLLLPDHFDQRLYAQDTLILES
                     SGATVVGDVNTFCALVFYEDIQGIEGRFISEGELMTRMVHLVTVANTLTLGAAGGYSG
                     EEAINAEFDLLKANTDYALVGYQVSAECAAIRWRGPDSGNLGVGGPGSETDKDVTGAW
                     FLRLARFYQMPLIPVFNSANKGGTFLDGAQDENAAAVTVNSIFAELAPR"
     gene            complement(6488..6913)
                     /locus_tag="AC16CX_11695"
     CDS             complement(6488..6913)
                     /locus_tag="AC16CX_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013210.1"
                     /translation="MAIKVKPLADISEKFVRVAATRQQDYEAGVKDPGTDWATATAAA
                     AETYNAGIQEAVQRNAFAKGVNNAGTAKWQKNVAGKGVQRWAPGIRDSRADYEAGFGP
                     YREALERVTLPPKGPRGDERNYQRSVAVGRALADVRRKS"
     gene            7275..7496
                     /locus_tag="AC16CX_11700"
     CDS             7275..7496
                     /locus_tag="AC16CX_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3013211.1"
                     /translation="MGEMSITVRKIIRTGDSLAVVLPKAYLKLMQLHHGDSVVLSATR
                     LHNATALELYKLDEGLLLSLRADRAAPKV"
     gene            7500..8363
                     /locus_tag="AC16CX_11705"
     CDS             7500..8363
                     /locus_tag="AC16CX_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3013212.1"
                     /translation="MAKPRLLTLPIALLDEPSNPARTAMDTAQLESLAQSLREVGLIE
                     PLVVEATDGGRYRVSAGHRRLLAARLAGLLELPCVLHARAGLADAVKIHENVEREELS
                     PVDEGRYYHELYLANGEDLGKVASLVRKALAYVDRRIQLVINDPDVREALRDGKIALT
                     VAEELIRVRDPEERSYYLEYAIRGGCSARQAREWRAQANARTEQRERAAAAAFGTTAT
                     SPAAAAAPLPGSQYLHLAAPHELSGSKDLRRCLYCDDEHEEWRMYRKFVCGACADRHL
                     APLEKERPSHG"
     gene            8356..8703
                     /locus_tag="AC16CX_11710"
     CDS             8356..8703
                     /locus_tag="AC16CX_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013213.1"
                     /translation="MGNKRSACGCRLIASPIIGAPAISEQCADHALGPSALVLLKRLC
                     GARELLDTIESCPVVEGEVPGSTIVRQLALIGLAVEEMESGAGLSGATVARLRNARLL
                     PDWMMMAAKRVAS"
     gene            8749..8973
                     /locus_tag="AC16CX_11715"
     CDS             8749..8973
                     /locus_tag="AC16CX_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013214.1"
                     /translation="MPVRKRRHTEAEFDLVAVRKLRRLARRVVAGLDEIERYLVKMNR
                     PDKPQAGSPAEIEAATEVYRAAADFVDGRQ"
     gene            8980..9219
                     /locus_tag="AC16CX_11720"
     CDS             8980..9219
                     /locus_tag="AC16CX_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013215.1"
                     /translation="MRARVLARGERTCHWRVLFATGTEVIVSGATARRAGAAAKAAGY
                     RRGGRGAIVGVEHACPAGCTRPLRDRVLLFDGEEG"
     gene            9223..9441
                     /locus_tag="AC16CX_11725"
     CDS             9223..9441
                     /locus_tag="AC16CX_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013216.1"
                     /translation="METPEDVARRTARVSQDLRAGCELGRCVDVSTDALTRATFRPGD
                     RVRDTVTGLEVTVVHSRFVLAVVPPSDR"
     gene            9726..9977
                     /locus_tag="AC16CX_11730"
     CDS             9726..9977
                     /locus_tag="AC16CX_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013217.1"
                     /translation="MTVVLVPAEWRFARQRYAWHQAERVVTRWRLSAGLVVITRRETA
                     PIGVSLGRHMRALDEQLETLGSTADVELRDRRLAKRRRP"
     gene            10393..10611
                     /locus_tag="AC16CX_11735"
     CDS             10393..10611
                     /locus_tag="AC16CX_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013218.1"
                     /translation="MSDEATRLPPIPDRLLVPAAKNLVIAWLRGLVLPERYASRALRE
                     WSRYVGVQLEAQDYVGLRGGPEHLGPEE"
     gene            10612..11082
                     /locus_tag="AC16CX_11740"
     CDS             10612..11082
                     /locus_tag="AC16CX_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013219.1"
                     /translation="MPEGERLKPWYEGLPEHEARIWRRYLEQYERDFHRFEYNVRVGR
                     GLSPAVPAPGAERPDEAAYRAMWTQLTQRRIDVVGFRLDETWLIEVEGRPSARAVGQL
                     VMYEELYTQQRPDTGPVQLAVVANAVSPDVATVLEQLGVLVFLYPEAPEEPSAA"
BASE COUNT         1690 a         3838 c         3926 g         1880 t
ORIGIN      
        1 ctcgttgtag atccgcgggc gatactgcgc gttgagccat cgcgtgagcg gttccgctac
       61 cagcacgcgc agtacgggcc ctatcgcgcg ccgggccgca gagccgaggc ccgcggaggc
      121 ggccaccacg tcgtcaaggt cggcgattgc cccgttgaag tagtcgccga ggggcccgcc
      181 aaagtagagc ccctcgagcc agccctctat cccgagccct accgtctgcc cgagtaccgc
      241 ttccgccgcg cgaagtccgt cgtccgggcc gagcgcgccc gtggggcgga tcttcgacag
      301 cagcccgtcg agcacggcct ggccgacctt gttgcgtagg ccggccccgg cggcgccgcg
      361 gcccggcacc gcttccgcgg gcgacacggg gatgtcgaag aggtctgaaa tcccggtcgc
      421 tgcgagctgc cccacgatgt cgccctgttc gcgttgcgcg tccgaccacg cctcgagcag
      481 tagcttggcg gcgaccgttt gccacttgag cgagtacgcc atgatggtcc cgacagcgcg
      541 cgtcgccagg tcctcggcga tctgcccgaa acccgaacgg ccctcctcga tcgtgagggc
      601 ccgtagcacc gtgtgcgtga ggtccgtcca gtcgtgggcg accgtcgtca ggctcacgtt
      661 tgggatttgg atccctggcg gcggcatgcc cccccctaca ccttgtctcg gaagatccgc
      721 aggacgattg cgtgcgtcac gtcggcgacg ggacggcgcc ggatctcgcc cgttgcgagc
      781 tgccgatcga ggtctgcgtg cgtgtttggc gggtactcac gaagaagctt gtccttctcg
      841 tggtcctcga acgttgacac gccattctgt acgatccacc gcccgatcgt tgagacatag
      901 ggcgccatcg agccgaagag ccacttggga ttgagcagcc ccgccggggt cgtgatctgc
      961 cacaggaaat cgagtacctc attgagccag cccgtgagct tgctaaagcc ctggacgatg
     1021 cggcgctcga gatccgcgag ccaggcgtcg gccttgagcg cccattcaag gccgagcttg
     1081 ccgaggagct gtagcacccg ccgcgccatg ccgatcgcgt cgaggatggg cgagacgacg
     1141 tgtgtccaca gccacgtgag cgccttctgg agcttgacga ggatttcgat tacgggcccg
     1201 aaaacgcgct ttagggccgc ggacaccttt tcggcggccg tccagagtgc ctgagcgatg
     1261 ggcttgacgt atcgcgtcca gaggtagtca accccgttgc gaacccaccg cgcaaactcg
     1321 gccgcgtagc gcgccgcttc cttcacgacg tgccagatct gcatggcggc ttgcttcgcc
     1381 gcttcccaca ggaaaatggc gagctcggcc gccttctcgg cgacgtaaac gatcgcgttc
     1441 cagatccagc cgagaaccgc gcccatggct atccgctgat gccgagctct tcaaacttgc
     1501 gagcctcgtc cgccaggtcc cgcttgacgc gctcggggat gaactcggcg ggcgtgtagt
     1561 ccttcgagaa cggcccctgc tctccgacga agaatgaaac gcgcacgaca cgctgtgtct
     1621 cgccgcgggg cgagagtcgg aacgtctcgt caacgcgcac gatctttgct tcggtcatta
     1681 cctgcctccg tttcgttccc gcgtgggcgg ctctgccggc ggggtttctg cgggcggggg
     1741 cggcggcggc tcttcggcag gctcgcgcct gccggcgccg cggtcccctc cgccgcgctc
     1801 gtcgtcgtct ctccacggcg ccttgccgcg catgcgttcg gtgtcgccac gcatgcggtg
     1861 cgccgggccc gtgacgtgat cggtgaggta atacccgggg ggcggttgct ctgcctcgcc
     1921 gggatgcacg ggatacctgc cgttcacgag ttgcgtaaag tcgccgcctg tgagggtccc
     1981 gcccgctgcg tccgcttcct cttcggtgtc gtaggcgtgg agctcgcgca tgtggcgctc
     2041 gctctcgtcc gcgccgctcg cgagctcggc aatcccggag tggcgggcct ggtcggcacg
     2101 gctggcgtgt gcctcgatga tcggcgccag gctcatgagc tcggccgcga gcgcctcggc
     2161 ggcagagacg aggcccgtac cgccgcggcg agaatcgaag gggcttacct caaagggatc
     2221 caccgcgcca ctccccgagc atcataggct atcccggctg gcccgcctgg cgccgcgcct
     2281 cgcgctgaaa ggcgtgcgcc gctcgagcca gggtttctat cttagccagg ttggccatcc
     2341 cgcgccggta acgcgtggga tccacaaacg gcccgagtgc gtgggcctcg acgatccgct
     2401 ctagaaagcg cgacaggtcc ggcaggagca acgcgagcgc ggccagcgag acgaggcttt
     2461 cctgcgtccg ctggtactcc tcgtcactca tgcgggcggc ggggacggcc gtggcgtcag
     2521 ccatcgccta cttgccccgc ctgggtacta cccggcgccg gcggccggta gtacctttct
     2581 tgggatcccc cgcctggtcg tgggtagtac ccgggcgatc gcccggggcc ttgccgagct
     2641 tggcggcgcc gtcccgcgca cgctcgaggg cggcgtactt gatccacggc ccgagggcgg
     2701 ggcttgtcgt gtaccacggc ctctcgcggt aggcggcctt ggcgagctcg gcgagctcct
     2761 cggccgtcag tcggatgctg aacacgaagc cgcggcgccc gccgcgggag agctgccggc
     2821 gccccaccgg gggggtcgcg ctcactggcc cctccgctgc cgcgcgagca cgccttgtct
     2881 gacgttgtgc gcggcgacca cgacgttgag ggcgaagccc acgcgagaaa accacttcgc
     2941 agcgcgggga tggtcacgac gccggagctc gcggcaggcg agcgtggtgc cggccgccat
     3001 gccgagctcc cacgcgacga gggcgccgcg gtcttggagt agtgggttga gctcgcgggc
     3061 gccctgggcg cggccccata gcgtcgtgcc cgcgttgagc ccgttgcccg caaagatcca
     3121 agctgcatcg ccggccgcag ctggcgccgc gagggcggcg acgagaacga gaacggcggc
     3181 cgatcggatc atgggtcctc cctggtaata cccgtcatgg gtatcatagg gggcctgtca
     3241 agtcctcgcg gtcttcctcg tcctcgagct cgtcttgcgc ctcgcgccac agcaccgcgt
     3301 agtcgagatc ctcgcgggcc tcgtcgcggt ctgtctcggg gtcctcgtcg ccccatccga
     3361 ggattcgctt cgccaggcgc acgcggccga cccagagcac gaggcgtgcc gccgcgcgcg
     3421 ggagaagaat ccgcgcggca gcgccgacga tggccccgat catccgtgag tcccgcggta
     3481 gtgccgctag acgtcgaggt gctcttcagt caccagccgg atcgccgaaa actggtccgc
     3541 ggcctgtagt agcgccgtga tcgtgcgaat gcgccagccc ccgcgcatcc aaaactctgc
     3601 cgggacggtg attaccgcgt cgttggcgcg taccgcatgc tcgaagccga tgggcgccac
     3661 catgtagtgt cgcgtggtgc tcgcgggctg gccgtcgaac gcaacctcgc gccacagaat
     3721 gtctaccccg tcgtcaatga tgagctccag cgagcggttg gctacggtga cgctggtcac
     3781 taggtcgaag taaagcaact gaagccgcgc gcgccgggcg gtcgggatgg tgatgctgat
     3841 ttccgcgccg gccgcagggt tggcaacggc cgtcacgacg agggcgcgcc gcgggtccgg
     3901 gcccgcggcc gatgtgggcg ccggccaagc aagaagcgtt tcggtcgtga tgtagccttc
     3961 tacgagcacc tgcacgcgct cgggcgtgat gggcccgccg tggaacacgc gcacggctgc
     4021 gtagtgctgc ccgcgcatcg gcgccccgcc ctcattctcg acggtgagcg cgagcaagaa
     4081 tccctcaccc acttgcacgc gaagcgttcc cgccgtgccg ttagcgctcg tcgccaggcg
     4141 ccacgtgttc ggcacgacgt cgccgccggg cgagagcatc cgcactcgcg ccactgcggt
     4201 catgcccgcg acggatccgt aggtccggag ctcgagctca tcatctcgcg agacgtagag
     4261 cgcgcccggg aacgccgcgc ggaagcgatc gaaggcaacc ggatccgggc gccccatcgc
     4321 ttcgcgaagc gcctgcgcca tctgtgcgta ctcttcagcg gtccacatgg gtcactcctc
     4381 ttccgaaact agagtgcgtc ccttgccctc cgcacgctgg cgcgtcgggc gccgtgggcg
     4441 cgggtggtcg ggatcaaagt cgaggtcgta ctctggattc tcgtcgcgcg ggttgtcgat
     4501 cggtagatag tcgcgcgggt cgtaggtcgg cgagcccggg tccgggtcgt accccggatt
     4561 gaccgcgggc acgcagcgat gcagccggtg cgagtggcct ggtgtcgcct cgacgtagac
     4621 atagccacac gagttgcagc ggaagcgccg ggcgctcatg cggagatggc ctcttcaaca
     4681 aaccagtccg aggccgaaaa ctggtccccc cccgcgccgc tcacccggac ttggagtgtt
     4741 gcgttggcgt tccaccacac attgagcgga atcggcagaa gctcggttgc gatcagcccc
     4801 tgcacgttgc tcgtggcccg gtcgctaaag tgatatgccc gcgttccgct cggcgggtgc
     4861 tccaccgtgc ttagcgcgat ggtgacttgc tgcccgccga gcgcaataac tgccaagctg
     4921 acgagtcggt ttcccgccac agcggtggtg gtcaggctgg cgcgaatccc cttgatgcgc
     4981 caccgtagca gcgatggaat gctcaactgg atcggctgcc cggcgaaggg cgccggctgt
     5041 gtgaatgtgc gctcatggcc cggcccctcc tcggccgagc gcaccattcc ttgcggccag
     5101 cccaccggct gataaagcgt cacgtagtcg gcgcagagga tcgcgtgctc ttggtcgatc
     5161 ggcccgagcg aatgggtgag ctcgacaatc gcatagaggt caccgcgctt ggcgcccccg
     5221 gctgccgcgc gtacctcgac gccgagtagc tgcccctcgc gaagcggaaa ccgggtcttc
     5281 gagaaaccag acgacgccgg gaacgcgggt gttcgatagg tcatgtgctg gacgacgcct
     5341 aggggcgtga gcaatcgcac gagcactaaa ggcgggtctt gcgcgagctc tgagtggatc
     5401 tcggtcacgg tcaagtagtc gtcgcgcccc acatacagcg ttgtcggcgg ctggacgttc
     5461 cggcgggcga agatgacgcg cgcaagctcc gggtttccga gtgcgtgagt caacgggcct
     5521 ccgcgcccga ggtattaccg ccctcgagct cgagcatggt actaccgggg cccggagatc
     5581 taaaaccccg ggccccggct gtcgagcggg ccctagcggg gcgcgagctc ggcgaagatg
     5641 ctgttgaccg tgacggccgc ggcgttttcg tcctgggcgc cgtcgaggaa ggtgccgccc
     5701 ttgttcgcgc tgttgaacac ggggatgagc ggcatctggt agaagcgcgc caggcgcagg
     5761 aaccacgcgc ccgtcacgtc cttgtccgtc tcggagcccg ggcctccaac gccgaggttc
     5821 ccggagtccg ggccgcgcca gcgaatcgcg gcgcactcgg ccgacacctg gtagccgacg
     5881 agcgcgtagt ccgtgttcgc cttgagcagg tcgaattcgg cgttgatcgc ctcttcgccc
     5941 gagtacccgc ccgcggcgcc gagcgtgagg gtgttggcga ccgtgacgag gtgcaccatg
     6001 cgcgtcatga gctcgccttc gctgatgaag cgcccttcga tgccctgtat gtcctcgtag
     6061 aacacgaggg cacagaaggt gttgacgtcg ccgaccaccg tcgcgccgga gctctcgagg
     6121 atcagcgtgt cctgggcgta gagcctctgg tcgaagtgat cgggaagcag cagctgcacc
     6181 gtcgaggcgg cgttgaggaa gcgcagcccc tgcacgttgt cgtgcatctt cggggagcgc
     6241 agccggtgca gcacggtcgc ctcctggacg tccgcccaca tctggagcag cctgatgtcc
     6301 gcatcggggc ggctgttgcg gacggtgagg gagttgccgg ccgccatctg gacgcctgtc
     6361 atggcggctg tggcgccggt ggtgaagccg gtgaggagct cgagtccgag tcccattttc
     6421 gtttgcctcc aaagcgaagg gcgccggtag tacccggcac cagtgaacgg ttacgctcga
     6481 gctcggacta gctcttgcgc cggacgtccg cgagggcgcg gcccacggcc acgctccgct
     6541 ggtagttgcg ctcgtcgccg cgcggcccct tcggcgggag cgtcacgcgc tcgagggctt
     6601 cgcggtaggg cccgaagccc gcctcgtagt ccgcgcgcga gtcgcggatg ccgggcgccc
     6661 agcgctgcac gcccttgccg gcgacgttct tctgccactt cgccgtgccc gcgttgttga
     6721 cgcccttggc gaacgcgttg cgctgcaccg cttcctggat gcccgcgttg tacgtctcgg
     6781 ccgcggcggc ggtcgccgtg gcccagtccg tcccggggtc cttcacgccc gcctcgtaat
     6841 cctgttgcct ggtcgcggcg acgcgcacga acttttccga gatgtccgcg agcggcttga
     6901 ccttgatcgc cattgcttcc cccctggcgg cccgtgctat ggtcgggccg catgacgagc
     6961 gccaggggcg gcgtgcgggg gatgggcccg cgaagccggg ccacctggcg cccgagtgtc
     7021 caagctgagc ctacagacgt tttccacagg ctgtcaacag cttttcaaca gcttttcaac
     7081 agattcatcc acaggctacc gtcgtcgacc cattagagtt agcggtcaga gggccgtttt
     7141 ctgttcgcat tcgcagtgga cgggggggct tgcatctctc tttaaactga gggaaggcgg
     7201 ggcgggggag gtgggccatc ccgcctgacg cgttcgcgag ctcttgactt ccgcccgtcc
     7261 ctggtgtgag catcatgggc gaaatgtcta tcactgttcg aaagatcatc aggacggggg
     7321 actctctcgc cgtcgtgctg cccaaggcat acctcaagct gatgcagctc caccacggcg
     7381 acagcgtggt gctgagcgcg acgaggctgc acaacgccac ggcgctcgag ctctacaagc
     7441 tcgacgaggg actattgcta agcctccgcg cggatcgcgc cgcgcccaag gtctgagccg
     7501 tggcgaagcc gaggctgctc acgcttccga tcgcgctact cgacgagccg tcgaaccccg
     7561 cgcgcacggc gatggatacg gcacagctcg aatcgctcgc acagtcgctc cgcgaggtcg
     7621 ggttgattga gcctctcgtc gtcgaggcga cggacggcgg gcgctaccgg gtctcggcgg
     7681 ggcatcggcg actcttggcg gcgcgcctcg cgggactgct cgagctgccg tgcgtgctcc
     7741 acgcgcgcgc gggcctcgcg gatgcggtca agatccacga gaacgtagag cgcgaggagc
     7801 tctcgccggt ggatgagggc cggtattacc acgagctcta cctggccaac ggcgaggatc
     7861 tagggaaagt cgcctcgctc gtccgcaagg cgcttgccta cgtagaccgg cggatccagc
     7921 tcgtcatcaa cgaccccgac gtgagggagg cgttgcgtga cggcaagatt gcgctcacgg
     7981 tcgccgagga gctcatccgc gtccgcgacc ccgaagagcg cagctattac ctcgagtacg
     8041 cgatccgtgg cggctgctcg gcgcgccagg cgagggagtg gcgagctcaa gcgaacgctc
     8101 ggacggaaca gcgggagcgg gcggccgcgg ccgcgttcgg tactaccgcg acgtcgccgg
     8161 ccgcggccgc cgcgcctctc ccgggtagcc agtaccttca cctggcggcg ccgcacgagc
     8221 tctcggggtc gaaggatcta cgtcgatgcc tatactgcga cgatgagcac gaagagtgga
     8281 ggatgtaccg caaatttgtt tgtggcgcgt gcgctgatcg gcacctggcg ccgctcgaga
     8341 aagagaggcc gtctcatggg taacaagcgt tcggcgtgcg ggtgcaggct catcgcgtcg
     8401 ccgatcatcg gcgcgccggc catctcggag cagtgcgcgg accacgcgct cgggccgagc
     8461 gcgctcgtgc tcctcaagcg actgtgcggc gcgcgagagc tcctcgacac gatcgaatcg
     8521 tgtcccgtcg tcgagggtga ggtaccgggg agcacgatcg tccgtcagct cgccctgatc
     8581 ggcctcgccg tcgaggagat ggagagcggg gccggcctct cgggcgcgac cgtcgctcgc
     8641 ctccgcaatg cgaggctgct gcccgactgg atgatgatgg cggcgaagcg ggtggcgtct
     8701 tgacaggcgt gctatgatat agaagatagg tattaccgga tgggtggcat gccagtcaga
     8761 aagcgccggc acacggaagc ggagtttgat cttgtcgccg tccgcaagct gagacgcctc
     8821 gctcgtcgcg tcgtcgcggg cctcgatgaa atcgagaggt atctcgtcaa gatgaaccgt
     8881 ccggacaaac cgcaagcggg tagccccgcc gagatcgagg cggcgaccga ggtatatcgg
     8941 gcggcggccg acttcgttga tgggcggcag taggacctta tgcgcgctcg cgtgctcgcc
     9001 cgcggcgaga gaacgtgcca ctggcgcgtg ctcttcgcga cgggcaccga ggtaatcgtg
     9061 tccggcgcaa ccgcaaggcg ggccggcgcc gcggccaagg ccgccggcta ccgccgcggc
     9121 gggcgcggcg ccatcgtcgg cgttgagcat gcctgcccgg ccggttgcac gcgcccgctc
     9181 cgcgaccgcg tgctactctt cgacggcgag gagggctagc ccgtggaaac ccccgaggat
     9241 gttgcacggc gcaccgcccg ggtgagccag gaccttcgcg ccggctgcga gctcggccgt
     9301 tgcgtagacg tctcgactga cgcgctcacg cgcgcgacgt ttcggcccgg cgatcgcgtc
     9361 cgcgataccg tgacgggcct cgaggtgacc gttgtccact cccgtttcgt ccttgccgtc
     9421 gttccgcctt ccgaccggtg aggagctcga ggccgtagcc gtgcggctac cggacggccg
     9481 gatcgtcctc cgcacggcga gcgagctcga gcgcttgccg ggaagtgcgg ccgcacttcc
     9541 gccccccgca aaacctgcgg ctcgctagga tgccgccagg ggcgcgcggg cggggggcct
     9601 gggcctggaa ccttcggcgg gtcgctgtga gcgccctggc gggaaccctg gcggccgtcg
     9661 ctcggccgtg ggccgtggtg cgtgtgctcg cgggcctcgc gaggtcccct tgctggccct
     9721 tttcggtgac agtcgtgctc gtcccggccg agtggcggtt tgccagacag cgctacgcct
     9781 ggcaccaagc cgagcgcgtc gtgacgcgat ggcgcctgag cgccggactg gtcgtcatca
     9841 ccagacggga aacggcgccg atcggtgttt ccctcgggcg ccacatgcgc gccctggatg
     9901 agcagctcga gacgctcggt agtaccgcgg atgtcgagct ccgcgatcgg cggctcgcga
     9961 aacggaggcg tccatgagtg acgatcggcc ggcggccggc gcaacggcca ggcccccaaa
    10021 tgctctcgaa gctggcggga tgggccggac gcgccacggg gcgcggccgg cgctgtccgt
    10081 ggtcgcggcg ggtcttttgg agcccttggt gaacgccgct gtgcggcgcg cccaccaggt
    10141 cctcggtaat accgcagccg gcgcggcgca ggccgccgcg ccggcgtgct cgatctgtgg
    10201 ccagggcgcc cgcaagggct tacacgtggc gcccgtcttt cgcctccgcg ccgcaaccgg
    10261 cccagacgcg ccagacttcc tgctcgtccc ggttgaccat gccgggcgcc aggcccggcc
    10321 gctatgcctc gcgtgcgcta caagcatggt cgccgcgctc actgtacccg agctcaccct
    10381 cgaggtagaa gcgtgagcga cgaagcgaca cgtctgccgc cgattcccga tcggctgctc
    10441 gtgccggccg ccaaaaacct ggtgattgcg tggctgcgcg gcctggtgct gcccgagcgc
    10501 tacgcatcgc gcgccctccg cgagtggtcg cgatatgtgg gggtccaact cgaggctcaa
    10561 gactatgtgg gcctccgcgg cggcccggag cacttgggcc cggaggagta gatgcccgaa
    10621 ggcgaacggc tcaagccgtg gtatgagggc ctccccgaac acgaggcccg catctggcgc
    10681 cgctacctcg agcaatacga gcgcgacttc catcgtttcg agtacaacgt gcgcgtaggg
    10741 cgcggactct cccccgcggt gccggcgccg ggcgccgagc ggcctgacga agccgcgtat
    10801 cgcgccatgt ggacgcagct aacgcaacgc cgcatcgacg ttgtgggctt tcgcctggac
    10861 gaaacgtggc tgatcgaggt cgaaggacgg ccgtccgcgc gcgcggtcgg ccagctcgtc
    10921 atgtacgagg agctctacac gcagcagcgg cccgacacgg gccccgtcca gctcgcggta
    10981 gtggcaaacg ccgtctcccc tgacgtggcc acggtgctcg agcagctcgg ggtcctggtg
    11041 ttcctgtatc ccgaagcgcc cgaagagccc tccgcggcgt aggctcgggc ccgccgctcg
    11101 agcgggtaat acccggcaaa tccgttgtgg ccatgacgcg aaaaatgcgg cgttggcgtt
    11161 cggccgcgcc acacagccta gcccgtcagc cccggagaaa accgcgcgag ggcgaaggct
    11221 ttgccggccg cgaaacggcc cgggaaccgc gcaagttgcg cagaccatgg tctgcttgcg
    11281 acgcgaggcg attcggcgaa tcccaagatc tccaccgcac cggggccctt cacg
//
LOCUS       DBNGWZ010000396         6896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3070218, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000396 DBNGWZ010000000
VERSION     DBNGWZ010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3070218"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="AC16CX_11745"
     CDS             complement(<1..1290)
                     /locus_tag="AC16CX_11745"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HIR3013220.1"
                     /translation="MSAQAVRYEHDLLGDRPVPAGAYYGVHTLRALENFPITGTPISI
                     YPDLIAALARVKQAAALANNELGLLDDARAQAIVKACEEIREGSLLEEFVVDVIQGGA
                     GTSTNMNANEVIANRALELMGREKGEYRSLHPVDHVNMSQSTNDVYPTAVKVALHFGI
                     QRLTAAMAELRVAFTAKSVEFADVIKMGRTQLQDAVPMTLGQEFSTYAVMLEEDEQRL
                     KEAALLIHEINLGATAIGTGITAHPDYAPLVCRRLAEVTGVALVTASNLVEATQDAGA
                     FVQLSGVLKRVAVKLSKTCNDLRLLSSGPRAGLNEIHLPAVQAGSSIMPGKVNPVIPE
                     VVNQIAFEVIGNDVTVSFAAEAGQLQLNAFEPIIAHSLFKSLTHLRNGCDTLRARCVT
                     GITANREHLRRTVENSIGIVTALNPYIGYANATQVAQE"
     gene            1529..2440
                     /locus_tag="AC16CX_11750"
     CDS             1529..2440
                     /locus_tag="AC16CX_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3013221.1"
                     /translation="MTRVSTLFVALLVAGALASTASAQELTGTLKKIKDTGTITLGHR
                     DTSIPFSYYDDKQQVVGYAMDICYKVVDAIKAQLKLPKIDIKLNPVTSATRIPLMANG
                     TIDLECGSTTNNLERQKQVWFATTHFVTANRFVSKKASKLKTVNDLKGKTVVSTSGTT
                     NIKNATEINVEKKLGMNIIAAKDHAEAFLTVESGRAVAFFMDDILLYSLVANSKNPSE
                     WVISADALTVEPYGIMMRKDDVEFKKVVNGAINNLYKSGEINKIYDKWFLKPVPPKGI
                     NLNVPMSAVFKKVVAKPTDSGDPKDYQ"
     gene            2546..3304
                     /locus_tag="AC16CX_11755"
     CDS             2546..3304
                     /locus_tag="AC16CX_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3013222.1"
                     /translation="MKYNWNWSIFWDQAPDGTGVYFWWLISGLGWTLSTALLAWVIAL
                     ALGSMVGTLRTTPLAWVVRLGNAYVEFFRNVPLLVQMFLWYFVLPELLPKGLGDAMKQ
                     MTPPWASFWPAVLCLGFYTSARVAEQVRAGIQSLPRGQSMAGTAVGLTLPQTYRFVLL
                     PQAFRIILPPLTSEFMNIIKNSSVALTIGLVELTARARSMQEFSFQVFEAFTAATIIY
                     IMITLIVVFGMRWLEKRVAVPGLISAAGAPLPGH"
     gene            3354..4046
                     /locus_tag="AC16CX_11760"
     CDS             3354..4046
                     /locus_tag="AC16CX_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3013223.1"
                     /translation="MFSGFDFDVVARSAPYLFQEGMTFTLTLTAMAMSGGIIFGTLLA
                     MGRLSSIPLFNVPAAGYVNLMRSVPLVLVIFWFYFLVPWLGGWIIGAKEPIQVGAFWS
                     SVITFTMFEAAYYAEIMRAGIQSVARGQVSAGQALGLNYWQTMGQVVLPQAFRNMLPV
                     LLTQTIILFQDTSLVYVLSITDFLGAAARIAQRDGRLVEMYLFVAVVYFIISFLLSYL
                     VKHLQQRIAIIR"
     gene            4153..4881
                     /locus_tag="AC16CX_11765"
     CDS             4153..4881
                     /locus_tag="AC16CX_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3013224.1"
                     /translation="MIEFKNVSKWYDKFQVLTNCTTKVEKGEVVVVCGPSGSGKSTLI
                     KCVNGLEPFQQGEVIVNGESVGAKETNLSRLRSKIGMVFQSFELFPHMKVTANINLGQ
                     VKVLGRSVEEATAKSMSLLKRVGLETHAHKFPGQLSGGQQQRVAIARALAMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELAQEGMTMMVVTHEMGFARKVAHRVIFMDRGEIVEDA
                     TKEDFFGKPRSERAVQFLSKILHH"
     gene            complement(5010..5468)
                     /locus_tag="AC16CX_11770"
     CDS             complement(5010..5468)
                     /locus_tag="AC16CX_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013225.1"
                     /translation="MGAVMRAFSMVAVSWALALSGWATVPTHERTGMDVGGVLNRALE
                     TRSDEDHGGTVATIMRTMVNALVGEAVSHSMDDTDLMMTVLALENGSAGEPSRWRNPR
                     TGNVYSITSRRTYDETGGPCLEYSMEAVSGGKRDEVHGTACRQPDGSWTK"
     gene            complement(5538..5921)
                     /locus_tag="AC16CX_11775"
     CDS             complement(5538..5921)
                     /locus_tag="AC16CX_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013226.1"
                     /translation="MALIEHLEAFSRRDNRRFAEALSLPFVHLWQDGEIWRYEGRSDV
                     DLLKQYARAEIDEKTFGCTELDEARLILDWEDLKAFYVRFTRYSAGGDKAGRAEAIWV
                     AIRDGGFWKLKLRIGARRIESPREA"
     gene            complement(5948..6325)
                     /locus_tag="AC16CX_11780"
     CDS             complement(5948..6325)
                     /locus_tag="AC16CX_11780"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3013227.1"
                     /translation="MLQQTAADPADTDTLRRLNENFVRSVEMSDVRYFEEALAEDFLN
                     SNPDGSLVDRAGFLAQIARPAMISNLEAHDVRIRIMGDIAIIHARTTYRKPDGGVGAG
                     RYTDVWARQRGQWLCVSAHVTRG"
     gene            6404..>6896
                     /locus_tag="AC16CX_11785"
     CDS             6404..>6896
                     /locus_tag="AC16CX_11785"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3013228.1"
                     /translation="MDDDPGIARIANLLADPGRAKILWALIDGTTRPAGELAFAANIS
                     AQSASAHFAKLVDGGLLASEAQGRHRYYRIASADIADAIEALSSLSAARPRAPRSVLP
                     SSSVPVQFLRARTCYDHLAGETAVQILEGMLKAKWLVPEGRDFRVTRLGEKELAAFGV
                     DLDT"
BASE COUNT         1244 a         2208 c         2132 g         1312 t
ORIGIN      
        1 ctcctgcgcc acttgcgttg cgttggcgta gccgatgtag gggttgaggg cggtgacgat
       61 gccgatcgag ttctcgacgg tgcggcgcag gtgctcgcgg ttggcggtga tgcccgtgac
      121 gcagcgcgct ctcagcgtgt cgcagccgtt ccgcaggtgc gtgaggctct tgaacaggct
      181 gtgggcgatg atcggctcga aggcgttcaa ctgcaattgc cccgcttcgg cggcgaagga
      241 gaccgtcacg tcgttgccga tcacctcgaa ggcgatctga ttgaccacct cgggaatcac
      301 cgggttgacc ttccccggca tgatcgagga gcccgcctgc accgcgggaa gatggatctc
      361 gttcagcccc gcgcgcggtc ccgaggaaag cagccgcagg tcgttgcagg tcttggacag
      421 cttcaccgcc acgcgcttca gtacccccga cagctgcacg aacgcgccgg cgtcctgggt
      481 cgcctcgacg aggttgctcg ccgtgacgag cgcgacgcca gtgacctcgg cgagcctgcg
      541 gcacacgagc ggcgcgtagt cgggatgcgc ggtgatgccg gtgccgatgg cggtcgcgcc
      601 gaggttgatc tcgtggatca gcagcgccgc ctccttgagc cgctgctcgt cttcctccag
      661 catcacggcg taggtcgaga attcctgccc cagtgtcatc ggcaccgcgt cctgcagctg
      721 cgtgcgcccc atcttgatca cgtccgcgaa ctccacggac ttggccgtga acgccacgcg
      781 cagctcggcc atggccgcgg tcaaccgctg gatgccgaag tgcagcgcga ccttgaccgc
      841 ggtcggatag acgtcgttgg tgctctggct catgttgacg tggtccaccg ggtgcagaga
      901 ccggtattcg cccttctcgc ggcccatcag ctcgagcgcg cgattcgcga tcacctcgtt
      961 cgcgttcatg ttggtggaag tgcccgcgcc gccctggatc acgtccacca cgaattcctc
     1021 cagcaggctg ccctcgcgga tctcctcgca cgccttgacg atcgcctgcg ccctggcgtc
     1081 gtcgagcagc cccagctcgt tgttggcgag cgccgccgcc tgcttcacgc gcgccagcgc
     1141 cgcgatcagg tcgggataga tcgagatcgg cgtgccggtg atggggaagt tctcgagcgc
     1201 gcgcagggtg tgcacgccgt agtaggcgcc ggcgggcacg gggcggtcgc cgagcagatc
     1261 gtgttcgtaa cgcacggctt gggcggacat ggctggctcc tgagcggttt tgctgttagc
     1321 agcctgtaga gaccctaagt ccgacaggct gctgggcgga ctgcctctat gatatgaaaa
     1381 ttcagcgatg ttccccggcc cggcgtgggg cgtagcggcc cgcgctgcgc tggacaaggc
     1441 ggggccatta ggctagatta aattcccgtt tcaggcgcaa tacccccacc gaaccaacca
     1501 gttaagacca gccataaggg agtccaccat gacgcgcgtt tccacactgt tcgttgcgct
     1561 gctcgttgcg ggtgccctgg ccagcaccgc atccgcgcag gagctgaccg gcaccctcaa
     1621 gaaaatcaag gacaccggca ccatcacgct gggccaccgc gacacgtcca ttcccttctc
     1681 ctactacgac gacaagcagc aggtcgtcgg ctacgcgatg gacatctgct acaaggtcgt
     1741 ggacgcgatc aaggcccagc tcaagctgcc gaagatcgac atcaagctca acccggtgac
     1801 ttccgccacc cgcatcccgc tgatggcgaa cgggacgatc gatctcgagt gcggctcgac
     1861 caccaacaac ctcgagcgcc agaagcaggt gtggttcgcc accacgcact tcgtgaccgc
     1921 caaccgcttc gtgtcgaaga aagcgtcgaa actcaagacc gtcaacgact tgaagggcaa
     1981 gaccgttgtc tccacctcgg gcaccaccaa catcaagaat gcgaccgaga tcaacgtgga
     2041 gaagaagctc ggcatgaaca tcatcgccgc caaggaccat gccgaagcgt ttctgacggt
     2101 ggaatccggc cgcgcggtcg ccttcttcat ggacgacatc ctgctctaca gcctggttgc
     2161 gaactcgaag aacccctccg aatgggtgat ctcggcggac gcgctgacgg tcgagcctta
     2221 cggcatcatg atgcgcaagg acgatgtgga gttcaagaag gtcgtgaacg gcgcgatcaa
     2281 caatctctac aagagcggcg agatcaacaa gatctacgac aagtggttcc tcaagccggt
     2341 cccgcccaag ggcatcaatc tcaacgtgcc gatgagcgcg gtattcaaga aagtcgtggc
     2401 gaagccgacc gattcgggcg atccgaagga ctaccagtag ttaaaacgga cgcgctgcgc
     2461 cgttttaact gcgcgcgata cagcggacgg agcttgccgc tgatcgcgcg cttttctttt
     2521 cagagttcac gccaatccag gccacgtgaa gtacaactgg aactggagca tcttctggga
     2581 ccaggcgcct gacgggaccg gcgtctattt ctggtggctg atcagcgggc tggggtggac
     2641 gctgtccacg gccctgctgg catgggtcat cgccctcgct ctgggctcga tggtcggtac
     2701 gctgcgcacg acgccgttgg cgtgggtggt gcggctcggc aacgcgtatg tcgagttctt
     2761 ccgcaacgtc ccgctgctgg tgcagatgtt cctgtggtac ttcgtgctgc cggagctgct
     2821 gccgaagggc ctgggcgacg cgatgaagca gatgacgccg ccgtgggcgt cgttctggcc
     2881 ggcggtgctg tgcctcggtt tctatacctc ggcccgcgtg gccgaacagg tgcgcgccgg
     2941 tattcaatcg ctcccacgcg ggcaaagcat ggccggcacc gcggtagggc tcacgctccc
     3001 gcaaacttac cgcttcgtgc tgctgccgca ggcgttccgc atcatcctgc cgccgctcac
     3061 ctccgagttc atgaacatca tcaagaacag ctcggtcgca ctgaccatcg ggctggtgga
     3121 gctgaccgcg cgcgcgcgct cgatgcagga attcagcttc caggtgttcg aggccttcac
     3181 cgccgcgacg atcatctaca tcatgatcac gctcatcgtg gtgttcggca tgcgctggct
     3241 ggaaaagcgg gtcgcggttc ccggtctcat cagcgccgcc ggcgcccccc tgccgggaca
     3301 ttgattgagc gacgagtgac ggctgacgac tggcgaattg atcaaaccat aaaatgttct
     3361 ctggttttga ttttgatgtc gttgcgcgct cggcgcccta cctgttccag gaagggatga
     3421 cgttcacgct gacgctgacc gcgatggcga tgagcggcgg gattatcttc ggcacgctgc
     3481 tcgcgatggg gcggctctcc agcattccgc tgttcaacgt gccggcggcg gggtacgtga
     3541 acctgatgcg ctcggtgccg ctggtgctcg tgattttctg gttctatttt ctcgtgccct
     3601 ggcttggcgg ctggatcatc ggcgcgaaag aaccgatcca ggtgggcgcg ttctggtcgt
     3661 cggtcattac cttcaccatg ttcgaggccg cctactacgc cgagatcatg cgcgcgggca
     3721 tccagagcgt cgcgcgaggg caggtgtcgg ccgggcaggc gctgggcctc aactactggc
     3781 agaccatggg ccaggtggta ttgccgcaag ccttccgcaa catgctgccg gtgctgctca
     3841 cgcagaccat catcctgttc caggacacct cgctcgttta cgtgctttcg atcaccgact
     3901 tcctcggcgc cgcggccagg atagcgcagc gcgacggccg gctggtggag atgtacctgt
     3961 tcgtggcggt ggtgtatttc atcatttcgt tcttgctgtc gtatctggtg aagcacctgc
     4021 agcagcgcat cgcaattatc agatgaagct ttcagccatc agctttcagc tatcagccaa
     4081 gacaaacctg actgcctcaa gctgatagct gattgctgac cgctgaccgc taaaaggatt
     4141 gataccatgc caatgatcga gtttaaaaac gtcagcaagt ggtacgacaa gttccaggtg
     4201 ctgacgaact gcaccaccaa ggtcgagaag ggcgaggtgg tcgtggtgtg cgggccctcc
     4261 ggctccggca agagcacgct gatcaagtgc gtcaacgggc tggagccgtt ccagcagggc
     4321 gaggtcatcg tcaacggcga gtcagtcggc gcgaaggaga ccaacctgtc ccggctgcgc
     4381 tcgaagatcg gcatggtgtt ccagagcttc gagctgttcc cccacatgaa ggtgaccgcc
     4441 aacatcaatc tcgggcaggt caaagtgctc ggacgcagcg tggaggaggc gactgccaag
     4501 tcgatgagcc tcctgaaacg cgtcgggctc gaaacccacg cgcacaagtt ccccggccag
     4561 ctctcgggcg gccagcagca gcgggtggcg atcgcgcgcg cgctcgccat ggacccgatc
     4621 gcaatgctgt tcgacgagcc gacttcggcg ctcgatcccg agatgatcaa cgaggtgctc
     4681 gacgtcatgg tcgagctcgc gcaggaaggc atgaccatga tggtggtgac gcacgagatg
     4741 ggcttcgcgc gcaaggtcgc gcaccgcgtg atcttcatgg accgcggcga gatcgtcgag
     4801 gacgcgacca aggaggactt cttcggcaag ccccgatccg agcgcgcggt gcagttcctc
     4861 tcgaagatcc tgcaccactg atgccgaaac gggtgatagg tgataggagt tgacttccgc
     4921 gcgagcgcgg gtctcttatc acttatcact cgtcacttat cacgcctttc cgcaaaggcg
     4981 ggccgtcgat cggcgcaccc cggttcagct cacttcgtcc agctcccatc cggctggcgg
     5041 caggcggtgc cgtgaacctc gtcccgcttt ccgccgctca ccgcctccat cgagtattcg
     5101 aggcaaggtc ctcccgtctc gtcgtaggtg cgcctcgagg taatgctgta cacattgccg
     5161 gtgcgcggat tgcgccagcg ggacggctcg cccgcgctgc cgttttccag cgccagcacc
     5221 gtcatcatca ggtccgtatc gtccatcgaa tgggatacgg cctcgccaac cagcgcgttg
     5281 accatggtgc gcatgatcgt cgcaaccgtc ccgccatggt cttcgtccga ccgtgtctcg
     5341 agcgcccggt tcagcacgcc gccgacatcc attccggttc gctcgtgcgt ggggacggtc
     5401 gcccatcccg acaaagccag cgcccaggac acggcaacca tggaaaaagc cctcatgacc
     5461 gcgcccatgc cttcctctgt ccgcgtcaac taccatcaga gcctacagca gggcggaatt
     5521 gggcttgttg atatacatca agcctcgcgc ggtgactcga ttcgtcttgc gccgattctg
     5581 agcttcagct tccagaatcc gccgtcgcga atcgcgaccc atattgcttc cgcccttcct
     5641 gccttgtcgc ctcccgccga ataccgggtg aatctgacgt aaaacgcctt gagatcttcc
     5701 caatcgagaa tcaggcgcgc ctcgtccagt tcagtgcacc caaaggtttt ctcatcgatt
     5761 tccgccctgg cgtattgctt caataaatca acgtcgctcc ggccctcata gcgccagatt
     5821 tcgccgtcct gccagagatg gacgaatggc agcgacaggg cctcagcgaa tctccggttg
     5881 tccctgcgag aaaaagcctc gagatgctcg atcagcgcca tcaacggcgt gactgccacc
     5941 ggcaacctca gccgcgggtg acgtgggcgg agacgcagag ccactggccc cgctggcgcg
     6001 cccagacgtc ggtgtagcgg cccgctccca ccccgccgtc gggcttccta tatgtcgtcc
     6061 gcgcatggat gatggcgata tcgcccatga tcctgatgcg cacatcgtgg gcctcgaggt
     6121 tcgagatcat cgccggccgc gcgatctgcg cgaggaaccc cgcacggtcg acgagcgagc
     6181 cgtcgggatt gctgttcagg aagtcctcgg ccagggcctc ctcgaagtaa cggacgtccg
     6241 acatttcgac tgaccggacg aagttctcat tcagccgacg cagggtgtcc gtgtcggcag
     6301 ggtccgcagc agtttgctgt agcatggtcc acgcctccat caataagtga tggacaggat
     6361 tatccttggg gtgtaacgtc aacgtttcat ggacaagtga aatatggatg acgaccccgg
     6421 catcgcccgc atcgccaacc tcctcgccga tcctggccgc gcgaaaatac tctgggcgct
     6481 gatcgacggc acgacgcgcc ccgcaggcga actggcattc gccgccaata tctcggcaca
     6541 gtcggcaagc gcgcacttcg ccaagctggt ggacggcggg ctgctcgcgt cggaagcgca
     6601 gggacggcac cgttactacc ggatcgcgag cgccgatatc gcggacgcga tcgaagcctt
     6661 gtcctcgctc agcgcggccc gcccgcgcgc gccgcgcagc gtgctgcctt caagctcggt
     6721 gccggtccag ttcctgcgtg cccgcacctg ctacgaccac ctggccggtg agacggcagt
     6781 gcaaatcctc gaggggatgc tgaaagcgaa atggctggtc cccgaggggc gggatttccg
     6841 tgtcacccgg ctcggcgaga aggagctggc ggcgttcggc gtggacctcg acaccg
//
LOCUS       DBNGWZ010000397         3587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_674758, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000397 DBNGWZ010000000
VERSION     DBNGWZ010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_674758"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1307)
                     /locus_tag="AC16CX_11790"
     CDS             complement(186..1307)
                     /locus_tag="AC16CX_11790"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HIR3013229.1"
                     /translation="MLQFGEQQTTGAGSAPGLQPAARAPAPQPDDVQQLRRVNVADKR
                     VINGQTDVNQLVPFKYKWAWEKYLSACANHWMPQEIQMSRDIELWKDPRGLTEDERRI
                     VKRNLGFFVTADSLAANNIVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLQ
                     LDDGEIFNAYHEIPSIRDKDEFLIPFIDTLTDPSFRTGTPEADQKLLKSLIVFACLME
                     GMFFYVGFTQILALGRQNKMTGSAEQYQYILRDESMHCNFGIDLINTIKLENPHLWTP
                     EFRDELREIFKKGVELEYRYAEDTMPRGVLGLNAAMFKEYLRFIANRRCQQIGLDPLF
                     EGVANPFPWMAEMIDLKKERNFFETRVLEYQTGGALNWD"
     gene            complement(1453..3579)
                     /locus_tag="AC16CX_11795"
     CDS             complement(1453..3579)
                     /locus_tag="AC16CX_11795"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HIR3013230.1"
                     /translation="MSVRYAQYLPEFVKKGIESELLDRRLAQYDLKCLGAALDANRDL
                     KFTYLGLQILYDRYFLHVRGRRIELPQIFFMRVAMGLALNEPDDQREARAIEFYNVLS
                     SFDFMSSTPTLFNSGTRSSQLASCYLTTVSDDLDGIYEAIKENALLQKFAGGLGNDWT
                     PVRALGSHIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCSYLETWHLDIEEFL
                     ELRKNTGDDRRRTHDMNTANWVPDLFMKRVMEGAEWTLFSPSDVPDLHDKFGKSFEQA
                     YLEYETKCARGEMKLFKKVPALQLWRKMITMLFETGHPWLTFKDPCNLRSPQQHVGVV
                     HSSNLCTEITLNTGPDEIAVCNLGSVNMPAHLDISTGRMDMEKLRRTVGTAMRMLDNV
                     IDLNFYSVGKARNSNFKHRPVGLGIMGFQDCLHMLRIPYGSQAAVTFADRSMEAIAYT
                     AYQASTDLAEERGPYSTFKGSLWDRGIMPCDTLKLLAEERGGYVECDMSTALDWEGLK
                     KRVQQVGMRNSNCLAIAPTATIANITGLSQCIEPTYQNLYVKSNLSGEFTVVNEFLVR
                     DLKKLNLWDEVMIADLKYFDGSLAKIDRIPPEIRNLYATAFEIEPRWLVECAARRQKW
                     VDQSQSLNIYMAGASGKRLDDTYKLAWIRGLKTTYYLRTLGATSAEKSTVTTGQLNAV
                     PTGGFDPTATDPKFCSVDNPECESCQ"
BASE COUNT          583 a         1107 c         1153 g          744 t
ORIGIN      
        1 ctcccctcca gcccgtcgtc gaaacggagc cacagggtgt aattgctctc ggcacggcat
       61 tcggtgatgg tgtgcatcat gctctgtctg cgtctgcggt aacgccggtt cagcaggctg
      121 gaacccacag cccctccctg ttggttgata aacgagcgtc cttccccgga atctcctgct
      181 ccgcgtcagt cccagttgag cgccccgccc gtctggtact cgaggacgcg ggtctcgaag
      241 aaattgcgct ccttcttgag gtcgatcatc tccgccatcc acgggaacgg gttggcgacg
      301 ccctcgaaca gcggatcgag cccgatctgc tggcaccggc ggttggcgat gaagcgcagg
      361 tactccttga acatcgccgc gttcagtccc agcacgccgc gcggcatggt gtcctccgcg
      421 tagcggtact cgagctcgac ccctttcttg aaaatctcgc gcagctcgtc gcggaattcc
      481 ggcgtccaca ggtgcgggtt ctcgagcttg atggtgttga taaggtcgat gccgaaattg
      541 cagtgcatgg actcatcgcg caggatgtac tggtactgct cggcggagcc cgtcatcttg
      601 ttctgccggc cgagcgcgag gatttgcgtg aagccgacgt agaagaacat gccctccatc
      661 aggcacgcga acacgatcag gctcttcagg agcttctggt ccgcttccgg cgttccggtc
      721 ctgaacgagg ggtcggtcag cgtgtcgatg aacgggatca ggaactcgtc cttgtcgcgg
      781 atcgagggaa tctcgtgata agcgttgaag atctcgccgt catcgagctg caggctctcc
      841 acgatgtact ggtaggcgtg cgtgtggatc gcctcctcga acgcctgccg cagcaggtac
      901 tgccggcatt ccggcgcggt gatgtggcgg taggtgccga gcacgatgtt gttcgccgcg
      961 agcgaatccg cggtcacgaa gaagccgagg ttgcgcttga cgatgcgccg ctcgtcctcg
     1021 gtgagaccgc gcgggtcctt ccacagctcg atgtcgcggc tcatctggat ttcctgcggc
     1081 atccagtggt tggcgcaggc ggagagatac ttctcccacg cccacttgta cttgaacggc
     1141 accagctggt tgacgtcggt ctggccgttg atgacgcgct tgtcggcgac gttgacgcgc
     1201 cggagctgct gcacgtcgtc cggctgcggc gcgggcgcgc gcgcggcggg ctgcagcccg
     1261 ggtgcggaac cggcgccggt cgtctgttgt tctccgaatt gcagcatgtt cagtctcctt
     1321 tatcgctgtt ttaggtggac actttccggc gccgccaccg cttcttgatc agtgttgtcg
     1381 gtgacgcggt tattggcgcc gatgaagcat tcctcaaaaa aaccgtgaat cgtgagtctt
     1441 gattcaccct ccttactgac aggactcgca ctcggggttg tcgaccgagc agaacttcgg
     1501 atccgttgcc gtcgggtcga aaccgccggt cggcaccgcg ttcaactggc ccgtcgttac
     1561 cgtggatttc tcggccgagg tcgcgccgag cgtgcgcagg taatacgtcg tcttcagccc
     1621 ccggatccag gcgagcttgt aggtgtcatc gagccgcttg cccgaggcgc ccgccatgta
     1681 gatgttgagc gactgcgact ggtccaccca cttctgccgc cgcgcggcgc actcgaccag
     1741 ccacctcggc tcgatctcga acgcggtcgc gtagagattg cggatctcgg gcgggatgcg
     1801 gtcgatcttg gcgagcgagc cgtcgaagta cttcaggtcc gcgatcatca cctcgtccca
     1861 caggttgagc tttttcaggt cgcgcaccag gaactcgttc accaccgtga actcgcccga
     1921 gaggttcgat ttcacgtaca ggttctggta ggtcggctcg atgcactgcg acagccccgt
     1981 gatgttggcg atcgtcgcgg tgggtgcgat cgcgaggcaa ttggagttcc tcatgccgac
     2041 ctgctggacg cgcttcttca ggccctccca gtcgagcgcg gtggacatgt cgcactcgac
     2101 gtagccgccg cgctcctcgg cgagcagctt cagggtgtcg cacggcatga tgccgcggtc
     2161 ccacagcgag cccttgaagg tcgagtacgg gccgcgttcc tcggcgaggt cggtcgaggc
     2221 ctggtacgcg gtgtacgcga tcgcctccat cgaacggtcg gcgaacgtca ccgctgcctg
     2281 cgagccgtag gggatgcgca gcatgtgcag gcagtcctgg aagcccatga tgccgagccc
     2341 gaccggccgg tgcttgaagt tggagttgcg tgccttcccg accgaataga aattgaggtc
     2401 gatgacgttg tcgagcatgc gcatcgccgt gccgacggtg cggcgcagct tctccatgtc
     2461 catgcggccg gtggagatgt cgagatgcgc cggcatgttg accgaaccca gattgcacac
     2521 cgcgatctcg tcggggccgg tgttcagcgt gatctcggtg cagagattgg agctgtgcac
     2581 cacgcccacg tgctgctgcg ggctcctcag gttgcacggg tccttgaagg tgagccaggg
     2641 atgcccggtc tcgaacaaca tggtgatcat cttgcgccac agttgcagcg cgggaacctt
     2701 cttgaagagc ttcatctcgc cgcgcgcgca cttcgtctcg tactcgaggt aggcctgctc
     2761 gaagctcttc ccgaacttgt cgtgcaggtc cggcacgtcg gagggcgaga acagcgtcca
     2821 ttcggccccc tccatcacgc gcttcatgaa aaggtccggc acccagttgg cggtattcat
     2881 gtcatgggtg cggcggcggt catcgccggt gttcttgcgc agctccagga attcctcgat
     2941 gtcgaggtgc caggtctcga ggtacgagca caccgcgccc ttgcgcttgc cgccctggtt
     3001 caccgccacg gcggtgtcgt tcaccacctt gaggaacggc acgacgccct ggctcttgcc
     3061 gttggtgccc ttgatgtgtg agccgagcgc gcgcaccggc gtccagtcat tgccgagccc
     3121 gccggcgaac ttctgcagca acgcgttttc cttgatcgcc tcgtagatgc cgtcgagatc
     3181 gtcggaaacg gtggtgagat agcagctcgc gagctgcgag ctgcgcgtgc cggagttgaa
     3241 cagcgtcggc gtcgagctca tgaagtcgaa gctcgagagc acgttgtaga actcgatcgc
     3301 gcgcgcctcg cgctggtcgt cgggctcgtt cagggcaagg cccatcgcca cgcgcatgaa
     3361 gaaaatctgg ggcagctcga tgcgccggcc gcgcacgtgc aggaagtagc ggtcgtagag
     3421 gatctgcaac ccgagatagg tgaatttgag gtcgcgattg gcatcgagcg ccgcgccgag
     3481 gcatttgaga tcgtactggg cgagtcgccg gtcgagcagt tccgactcga tgcccttctt
     3541 cacgaattcc ggcaggtact gcgcgtagcg cacgctcatt tccgcct
//
LOCUS       DBNGWZ010000398        14979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1178899, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000398 DBNGWZ010000000
VERSION     DBNGWZ010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1178899"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /gene="asd"
                     /locus_tag="AC16CX_11800"
     CDS             <1..1155
                     /gene="asd"
                     /locus_tag="AC16CX_11800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3013231.1"
                     /translation="GWRGMVGSVLMERMRAERDFELIEPVFFSTSRMGAKGPEIGKSA
                     LPVRDARDLTGLAEMDILVSCQGSEYTKEIHPRLRAAGWEGYWIDAASALRMEQDAVI
                     ILDPVNKNVIEQGLAEGIRNYIGGNCTVSLMLMALGGLFKAGFVEWMTAMTYQAASGA
                     GAQHMRELVQQMGKLHGAAKHLADDPAAAILEIDKAVTQAMRSESLPTEHFEFPLAGS
                     LLPWIDKDLGNGQSREEWKGMAEANKILGRASNPIPVDGICVRVGAMRGHSQALTITL
                     NKNLPLKEIEERLAAANDWVKVVPNEQGATLRELTPAAVSGTLTIPVGRLRKLPMGDH
                     YLAAFTVGDQLLWGAAEPLRRMLRILLEAGSGQPRAKKGRAARRKAAVSA"
     gene            1356..3875
                     /locus_tag="AC16CX_11805"
     CDS             1356..3875
                     /locus_tag="AC16CX_11805"
                     /inference="COORDINATES: protein motif:HMM:NF051932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="motility hub landmark protein FimV"
                     /protein_id="HIR3013232.1"
                     /translation="MRDFHGKTWLVALLTALLPCVAGAAGLGRLNVLSALGQPLVAEI
                     DLVAVTKEELGSLSARLAHPEAYQLANLQYNVALTGARLTLERRPSGQHYIKIVTTRS
                     VNEPFIDLLVELTWAAGRLSREYTALLDPPGITPAPPAPVISAVPESRPAPEPETQPT
                     VAATEPAVTRPVMTAPIAGGNEYGPVQRGETLGKIAKSVMPEGVTLEQMLVALYRNNP
                     EAFIRKNLNLVRTGRILRVPDRDEVASVARGEAVKEYRTHVADWNAYRQKLADAAGTV
                     PAEGRTAVSGKITTKVEDKAAPGEPKDVVRLSKGEPPGGAGKGKPRSGAERIRMLEEE
                     AVARERALKEANERIVQLEKTIKDMQKLVELKSPGMAAVQQQAQQATASKPEAKAAAK
                     PEAKKPEAVAVAKPEPAKPGTAKPEAAKPVEAPKPEAAAKPETPKPQAVAPAPKPAAK
                     PKPKPAPPPKKEPELMDQIMEALTDPLYLGAGGGVIVIGGLAFWMVRRRRAQAEAGYE
                     PPVPPKLSMAAAAAAGAAAGTAAATAAPEPAAAPAEDVDPLAEAEVYIAYGRDGQAEE
                     ILKEALGKNPKREDVQLKLLEIYSARKDKGAFGKLAGELNKLTGGAGDNWIKAAAMGY
                     ALDPANSLYAAGKDAAAVVTSTAAAADVDLDLGLGEAGTSTDITLEAGAAGAAAGTDT
                     AILDLGVSEKAREQPAAMPDFALEVPPAGGAQTDIALEAAAPAQDAGMIDFQIELPKS
                     AEEPATAAAPPPAPSADAGLDFKLEVPDLDLGEKTKAEPAAAGGEKDGHWYDVQTKFD
                     LAKAYQEMGDKDGAKEILQEVIKEGDAEQKAQAKTLLDSLG"
     gene            3969..4805
                     /gene="truA"
                     /locus_tag="AC16CX_11810"
     CDS             3969..4805
                     /gene="truA"
                     /locus_tag="AC16CX_11810"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR3013233.1"
                     /translation="MRIALGVEYNGSRFCGWQTQPQGCSVQDALERALAEVAGEPVAT
                     VSAGRTDAGVHALAQVVHFDTHAERPETAWVRGVNALLPAACAVNWSRQVADGFHARY
                     SAVARCYRYLLLNHPVRPAADQSRVGWFHAPLDLETMQHAARLLIGEHDFSAFRSAEC
                     QARTPVRTLTRLDIARRGDHLVFDVCANAFLHHMVRNIVGCLVYVGKGKYPPEWLGEV
                     LAGRDRSRAAPTFDAAGLYLSQVVYDARWGLPETPRGTLIDLGIPAQATGKRKPRARS
                     AV"
     gene            4842..5465
                     /locus_tag="AC16CX_11815"
     CDS             4842..5465
                     /locus_tag="AC16CX_11815"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3013234.1"
                     /translation="MATAVKICGITRPEDALAAARAGAHAIGLVFYAKSPRCVTPARA
                     AEIIRALPPFITTVGLFVDARAEEVRAALAQAPVELLQFQGSETPEFCRQFRRPYVKA
                     VRMRAGVDLLQYARDYHDAKALLLDNYVEGLHGGSGAAFDWSLIPRGLQLPVILSGGL
                     TPENVMEAVRRVRPSAVDVSSGVESAKGVKDAQKIAAFIKGVRNADV"
     gene            5458..6651
                     /gene="trpB"
                     /locus_tag="AC16CX_11820"
     CDS             5458..6651
                     /gene="trpB"
                     /locus_tag="AC16CX_11820"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3013235.1"
                     /translation="MYDLPDASGHFGPYGGVFVAETLVQALDELKAAYARYRADPEFQ
                     AEFAYELKHYVGRPSPVYHARRWSERFGGAQVYLKREDLNHTGAHKINNVIGQALLAR
                     RMGKPRVIAETGAGMHGVAAATIAARYGMECVVYMGTEDVKRQVQNVYRMKLLGATVV
                     PVESGSKTLKDALNEAMRDWVTNVESTFYIIGTVAGPHPYPMMVRDFQSVIGREALEQ
                     MQELAGRQPDAVLACVGGGSNAMGIFHAYVPDEKVRLIGVEAAGHGLESGKHAATLCT
                     GKPGVLHGNRTYLLQDENGQILETHSISAGLDYPGVGPEHAWLKDSGRAEYVSVTDEE
                     ALQAFHDLCRLEGIIPALESAHALAYAAKLAPRMKKDQILLVNLSGRGDKDMHTVAER
                     SGIKI"
     gene            6743..7558
                     /gene="trpA"
                     /locus_tag="AC16CX_11825"
     CDS             6743..7558
                     /gene="trpA"
                     /locus_tag="AC16CX_11825"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3013236.1"
                     /translation="MSRIAATFEALKRQGRKALIPFITAGDPDPKLTVPLMHALAKAG
                     ADVIELGVPFSDPMADGPVIQRSSERALQHHVSLRQVLGQVAEFRRTDRTTPVVVFGY
                     ANPIEALGLERFADAARAADADGVLVVDYPPEEARLLVELLDARGLDTIFLLSPTTTD
                     ERLTEVAKLGRGYLYYVSLRGVTGASHIDIADVAARVKHVRGYTRLPVGVGFGIRDAQ
                     SARAIGAVADAVVIGSALIQEIEKTPRDKVMARVEQFLSPIREALDSLSAVRA"
     gene            7617..8483
                     /gene="accD"
                     /locus_tag="AC16CX_11830"
     CDS             7617..8483
                     /gene="accD"
                     /locus_tag="AC16CX_11830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3013237.1"
                     /translation="MSWLQKLLPPKIKRDTGSPRKVVPEGLWSKCESCEAVLYRADLE
                     KNLHVCPKCSHHNRISARERLDCLLDREGRYEIGAEVVPVDSLKFKDSRRYTERLEEA
                     RSETSEEDALVVMQGSIKTIPVVAAAFEFGFLGGSMGSVVGERFVRGVQVCLEQRLPF
                     LCITASGGARMQEGLLSLLQMAKTCATLTQLARERLPFLTVLTDPTMGGVSASFAMIG
                     DVVIAEPGALIGFAGPRVIEQTMRETLPEGFQRSEFLLKHGAIDMIVDRREMRDKLAS
                     LVTILLKLPPAA"
     gene            8593..9876
                     /gene="folC"
                     /locus_tag="AC16CX_11835"
     CDS             8593..9876
                     /gene="folC"
                     /locus_tag="AC16CX_11835"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR3013238.1"
                     /translation="MNLESERRTLQQWLEYIERLHPKQIALGLERVSRVKQALGLEPR
                     FPIITVGGTNGKGSVCAMLEAILHHAGYCIGCYTSPHLLRYNERVRVARAEASDADLV
                     RAFSAVDSARGSVTLTYFEMGTLAAMRLFAERGVEAAVLEVGLGGRLDAVNAFDADCA
                     VITTVDIDHVDFLGGDREAIGYEKAGILRGGRPAVCADPAPPASLIRHAAEIGAQLLR
                     IGADFGASAQGRQWQYWGPRGKRNALPYPALRGACQLANAAAAVTAIECLRERLPVTM
                     NDIRAGLLQTDIPGRFQVLPGRPAVILDVAHNPQAARVLAASLDAMGRSGRTLAVFAM
                     LNDKDIAGVVAALKAHVAHWFVAGLGGPRGARVAELGHILAAAAISAVTRCEDVAVAY
                     AQACDMATENDRIVVFGSFYTVATVMQLRASRPGG"
     gene            9934..10626
                     /locus_tag="AC16CX_11840"
     CDS             9934..10626
                     /locus_tag="AC16CX_11840"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3013239.1"
                     /translation="MARTISDEELQLKRRARRRLIGAIVLVTAIVVVLPMVLDSEPKP
                     ASQEISIQIPSPDSGVFTSKVVPLPASPEAKPAPKTPQGKPAAAPPAEEAKKVAAAPP
                     PVAPAAPAPKQVAPEPAAKPEPSKPDDAAPAKKPAKATAKTAKKARPSPKPAAGRFVV
                     QVIALADAEKAKQMQEKIAASGIKSYTEVVKTAKGDVTRVRAGPFASRAAAEKAREQL
                     KTLGMNGNITTR"
     gene            10623..11114
                     /locus_tag="AC16CX_11845"
     CDS             10623..11114
                     /locus_tag="AC16CX_11845"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR3013240.1"
                     /translation="MTLFDIAVLVIIGLSVLLSVIRGLMREVLALAAWVVAFLAANLL
                     AGQVAPWLPAAIPTEELRLLAGFVGVFLAVLVAMSVLAIVASKLVKSAGLGLEDRLLG
                     GMFGLARGLLIVVILVLLAGLTSLPRQAVWRNAVLSDPLVAFAGEVKHWLPADLAQRI
                     TYD"
     gene            11124..12650
                     /gene="purF"
                     /locus_tag="AC16CX_11850"
     CDS             11124..12650
                     /gene="purF"
                     /locus_tag="AC16CX_11850"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3013241.1"
                     /translation="MCGIVGVVAKNPVNQLLYDGLMVLQHRGQDAAGIVTAEGPTFHM
                     HKGPGMVRDVFRTRNMRALTGFSGIAHTRYPTAGSAWSAAESQPFYVNSPFGIVLGHN
                     GNLTNTEQLKQELFRQDLRHVNTNSDSEVLLNVLAHELQENATNYKLDPATIFTAASG
                     VYRRCRGAYAVVAMIAGYGLLAFRDAHGIRPLVFGRAEAGQGYEYMVASESVALDALG
                     FQVVRDVAPGEAIFIDQDGTFYSRQCVPNATLNPCIFEFVYLARPDSVIDGISVYETR
                     LRMGVSLAEKIRRQYRHLDIDVVIPIPDSSRPAAMELSKQLSLPYREGFIKNRYIGRT
                     FIMPGQGMRRRSVRQKLNSIAMEFRGKNVLLVDDSIVRGNTSREIVQMARESGARRVF
                     FASAAPPVRYPNVYGIDMPTREELIACGRSEAEIAREIGCDELIYQDLDALREDVRSV
                     NPRIVNFEDSCFSGVYVTGDVTQEYLAGVEAQRRDGAKMAEMAAAASAQLDLGLELVE
                     "
     regulatory      12826..12903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            12959..14134
                     /locus_tag="AC16CX_11855"
     CDS             12959..14134
                     /locus_tag="AC16CX_11855"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071269 - L-homocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HIR3013242.1"
                     /translation="MSEDYQLDTLAVRAGTERSQFGEHSEALYLTSSFVFKSAAQAAA
                     RFSNQEPGNIYSRFTNPTVTMLEQRLAAMEGAECCVATSSGMSAILACVMGLLKSGDH
                     IVCSAGVFGATVQLLGGILKRFGVETTFVSGSDADAWQKAMQANTRLLYLETPSNPLM
                     EVYDIAALAKISKKASALLAVDNVFCTPALQRPLELGADIVIHSATKFLDGQGRVLGG
                     AVLGRRDTIYEGVFGFLRTAGPTLSPFNAWVILKGLETLKIRLQAQSASALELARWLE
                     QQPKIERVYYPGLPSHPQHELAKKQQKGFGAIVSFDVKGGRDAAWRVVDATRLLSITA
                     NLGDTRTTITHPASTTHGRISQEARNAAGIGDGLLRVAVGLESVEDIKADLARGLSA"
     gene            14158..>14979
                     /locus_tag="AC16CX_11860"
     CDS             14158..>14979
                     /locus_tag="AC16CX_11860"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3013243.1"
                     /translation="MGEAQFHGVFPYLVSPVEASGEVKSEVLERLCNDLIEAGVHGLT
                     PLGSTGEFAYLSWPQKKRIVEAVVSAARGRVPVVAGVASTTIADAVFQAREMERIGCN
                     GILAILEAYFPVTENGVFEYFKAVAEAVSLPVVLYTNPIFQRSDLTLPVIDRLSRIPN
                     VRYLKDASYNTGRLLSVINRVEGRLKVFSASAHIPACVMLIGGVGWMAGPSCLVPRQS
                     VRLYELCLRKDWDAAMRLQQDLWNLNEAFAKHNLAACIKGGLELQGYPVGVPLPPQ"
BASE COUNT         2692 a         4756 c         5111 g         2420 t
ORIGIN      
        1 ggctggcgcg gcatggtggg ctccgtgctg atggagcgca tgcgcgcgga acgcgacttc
       61 gagctgatcg agccggtgtt tttctccacc tcacggatgg gtgccaaagg accggagatc
      121 ggcaaaagcg cgctgccggt cagggacgcg cgcgacctga cgggcctggc ggagatggat
      181 atccttgtct cctgtcaggg cagtgagtac acgaaggaaa tccatccccg gctgcgcgcg
      241 gcgggctggg aaggttactg gatcgacgcc gcttccgcgc tgcgcatgga gcaggatgca
      301 gtcatcattc tcgatccggt caataagaac gtgatcgagc aggggctcgc ggagggtatc
      361 cgcaactata tcggtggaaa ctgcacggtg agcctgatgc tgatggcgct tggcggcctg
      421 ttcaaggcgg gattcgtcga gtggatgacg gccatgacct accaggccgc ctccggcgcc
      481 ggcgcacagc acatgcgcga gctggtgcag cagatgggca agctccatgg ggcggcaaag
      541 catctggcgg acgaccccgc cgccgccatc ctcgaaatag acaaggcggt cacgcaagcg
      601 atgcgcagcg agagcctgcc gaccgagcat ttcgaatttc cgctcgccgg cagcctgctg
      661 ccgtggatcg acaaggatct cggcaacggc cagagccgcg aggagtggaa aggcatggcc
      721 gaggccaaca agatcctcgg ccgcgcttcg aatcccattc cggtggacgg catctgcgtg
      781 cgcgtcggtg cgatgcgcgg ccacagccag gcgttgacca tcacgctcaa caagaatctg
      841 ccgctcaagg aaatcgagga acggctcgcg gccgccaacg actgggtgaa ggtggtgcca
      901 aacgagcagg gagccacatt gcgcgagctg acccctgcgg cggtttccgg gaccctgacg
      961 attccggtcg ggcggctgcg caagctgccg atgggggatc actatctcgc cgcattcacc
     1021 gtgggcgacc agctcttgtg gggcgccgcc gagccgctgc gccgcatgtt gcgcatcctg
     1081 ctcgaagccg gatcggggca gccccgcgcc aagaagggcc gtgcggccag gcgcaaggcg
     1141 gcggtgagtg cttgaaaccc cggccagcag ttattagacg ctggatcgca ttacagcgtc
     1201 ataacccgct tgcccggacg gcaaggcgtc ctatcccgct gaaatttgat taaaaatagt
     1261 cctgtcacgc gaaacactta gagggactct gagcttgcat cgctaactga ttgatgttag
     1321 cctgtttacc cctaaaaaat cataagggta ccatcgtgcg tgatttccac gggaagacat
     1381 ggttggtggc gctgctgacc gcgctgctgc cgtgcgttgc aggcgctgcc ggcttgggga
     1441 ggcttaacgt cttatcggcg ctgggtcaac cgctagtcgc ggagatcgac ctggttgcgg
     1501 taaccaagga ggaactcggc tcactcagcg cccgcctcgc gcatcctgaa gcctatcaac
     1561 tggcgaacct gcagtacaac gtggcgctga ccggcgcgcg cctgaccctc gaacggcgcc
     1621 ccagcgggca acattacatc aagatcgtca cgacccgctc ggtcaacgag cctttcatcg
     1681 acctgctggt cgagttgacc tgggctgcag ggcgcctctc gcgcgaatac accgcgctgc
     1741 tcgatccgcc gggcatcaca ccggcgccgc ccgcacccgt gatttccgcg gtgccggagt
     1801 cgcgtcccgc ccctgaaccc gagacgcaac ccacggtcgc ggcaaccgag ccggcggtca
     1861 ccaggccggt catgaccgcg ccgatcgctg gcgggaacga gtacggcccg gtccagcgtg
     1921 gcgagacgct cggcaagatt gccaagagcg tcatgccgga aggcgtcacg ctggagcaga
     1981 tgctggtcgc cctctaccgc aacaatcccg aggcgttcat caggaaaaac ctcaacctgg
     2041 tgcgcacggg caggatcctg cgcgtgccgg acagggacga ggttgcgtcg gttgcgcgag
     2101 gcgaggcggt gaaggagtac cgtactcacg tcgccgactg gaacgcctac cggcagaagc
     2161 tggccgatgc ggcgggaacg gtcccggccg aggggcgcac cgcggtgagc ggcaagatca
     2221 ccaccaaggt cgaggacaag gccgcccccg gcgagccgaa ggacgtagtg cggctgtcga
     2281 agggcgagcc tcccggcggg gcgggcaaag gcaagccacg cagcggcgcc gagcgcatcc
     2341 gcatgctgga agaggaagcg gtggcgcgcg agagggcgct gaaagaggcc aacgagcgca
     2401 tcgtccagct cgaaaagacc atcaaggaca tgcaaaagct cgtcgagttg aagagcccgg
     2461 ggatggccgc ggttcagcaa caggcgcagc aagcgaccgc gtccaagccg gaagcgaagg
     2521 ctgccgcgaa gcctgaagcg aaaaagccgg aagccgtggc tgtcgcaaaa cccgagcccg
     2581 caaaaccggg gacagccaag ccggaagccg caaaaccggt cgaggcaccc aaacctgagg
     2641 ccgccgccaa gcccgaaaca ccaaagccgc aagccgttgc accagcgccc aagcccgcgg
     2701 ccaagccgaa gccgaaaccc gctcctccgc ccaagaagga gcccgagctg atggatcaga
     2761 tcatggaggc gctgaccgat ccgctctacc tgggcgcggg cgggggtgtc atcgtcatcg
     2821 gcggcctggc gttctggatg gtgcgacggc gtcgcgcgca ggccgaagcc ggatacgagc
     2881 cgccggttcc gcccaagctg agcatggcgg ctgccgctgc ggcgggggca gccgccggga
     2941 ccgcagcagc aaccgccgcg ccggaacctg ccgccgcgcc tgccgaggat gtcgatccat
     3001 tggcggaggc ggaagtctac atcgcctatg ggcgcgacgg gcaagccgag gaaattctga
     3061 aggaggcgct tggcaagaat cccaagcgcg aggacgtgca gctgaagctg ctcgagattt
     3121 attccgcgcg caaggacaag ggcgctttcg gcaagctcgc cggcgagctc aacaagctga
     3181 ccggtggcgc tggcgacaac tggatcaagg cggcagcgat gggctatgca ctcgatccgg
     3241 ccaattccct gtacgcagcc ggcaaggacg cagcggcggt tgtcaccagt acggcggctg
     3301 ccgccgacgt cgatctcgat ctcggcctgg gggaagccgg cacttcgacc gatatcacgc
     3361 ttgaagcggg cgccgcggga gcggcagccg gcaccgacac cgcgatactc gatctgggcg
     3421 tgagcgaaaa ggcacgagag cagccggcgg caatgcccga ctttgcgctt gaggtgccac
     3481 ccgcaggagg tgctcaaact gacatcgcgc tcgaggcggc tgcgccggcc caggacgccg
     3541 gcatgatcga tttccagatc gagctgccca agtccgccga ggaacccgct accgcggcgg
     3601 cgccaccgcc agctccgagc gcggacgcag ggctcgactt caagctggag gttcccgacc
     3661 tggatctcgg ggaaaagacc aaagcggaac cggcggcagc tgggggagaa aaggacggtc
     3721 actggtacga tgtacagacc aagttcgatc tggccaaggc gtaccaggag atgggcgaca
     3781 aggacggcgc caaggaaatc ctgcaggaag tcatcaagga aggcgacgcc gagcaaaagg
     3841 cgcaggccaa gaccctgttg gacagtctgg ggtaatcggg aagacaaggc gacagatcga
     3901 accgcggcag tgtgaactgt cgcggttttt gtttttggga gccgcgatag caacaggact
     3961 gtgaaaacgt gagaattgcg ttgggtgtgg aatacaacgg cagccgcttc tgcggctggc
     4021 agacccagcc gcagggctgc tcggtgcagg acgcgctcga gcgggcgctt gccgaagtcg
     4081 ccggcgagcc cgtcgccacg gtcagcgccg ggcgcacgga tgcaggcgta cacgcgctcg
     4141 cccaggtggt gcacttcgat acccatgccg agcgcccgga aacggcgtgg gtgcgcggcg
     4201 tcaacgcgct gctgcccgct gcctgcgccg taaactggtc gcggcaggtt gcggacggct
     4261 ttcatgcgcg ctactcagcg gttgcgcgat gctaccgtta cctgctgctc aaccatccgg
     4321 tgcggccggc ggccgaccag tcgcgggttg ggtggttcca tgcgcctctc gatctggaga
     4381 cgatgcagca cgcggcgcga ctgttgatcg gtgagcacga tttcagcgcc tttcgcagcg
     4441 ccgagtgcca ggcgcgcacg ccggtgcgca cgctgacgcg gctcgacatc gcccgccgtg
     4501 gcgaccacct ggtgttcgat gtttgcgcaa atgcgtttct tcaccacatg gtgcgcaaca
     4561 tcgtcggctg cctggtctac gtcgggaagg gcaagtaccc gccggagtgg ctgggcgagg
     4621 tcctggcggg ccgcgaccgc agccgcgccg cgccgacatt cgatgcggcg gggctgtacc
     4681 tgtcgcaggt cgtgtacgac gcgcggtggg gactgccgga aacgccgcga ggcacgctga
     4741 tcgatctggg aatcccggca caggcaacag ggaaacggaa accgcgcgcg cgaagcgcgg
     4801 tatagttgga tcacgagtca ccgctcacga ttcacgagaa aatggcaacc gccgtcaaga
     4861 tctgcggcat tacccgcccg gaggatgcgc tcgccgccgc gcgcgccggt gctcacgcga
     4921 tcgggctggt gttctacgcc aagagcccgc gttgcgtcac cccggcgcgc gcggcggaga
     4981 tcatccgcgc cctgccgccg ttcatcacga cggtcgggct gttcgtggac gcgcgggcgg
     5041 aggaagtgcg ggcggcgctg gcacaggcgc cggtcgagtt gctccagttc cagggttcgg
     5101 agacccctga attctgccgg caattcaggc ggccctatgt caaggcggta cggatgaggg
     5161 cgggggtgga tttgctacaa tacgcgcggg attatcacga tgccaaggcg ctgctgctcg
     5221 ataactacgt cgaggggttg cacggcggca gcggcgccgc cttcgactgg agcctgattc
     5281 cgcgcggttt gcagctgccg gtcattctgt caggcggcct gaccccggag aacgttatgg
     5341 aagctgtgcg ccgcgtgcgg ccgagtgcag tggacgtatc gagcggcgtc gagtccgcga
     5401 agggcgtcaa ggacgcccag aagatcgccg cgttcatcaa gggagtgcgc aatgcggatg
     5461 tatgatctcc ccgatgcgag cggccatttc gggccgtacg ggggagtatt tgttgccgag
     5521 acgctggtcc aggcgctgga cgagctgaag gccgcgtacg cgcgctaccg cgctgacccc
     5581 gagttccagg cggaattcgc ctacgagttg aagcactacg tcgggcggcc gagtccggtt
     5641 taccacgcgc ggcgctggtc cgagcgcttc ggcggcgcgc aggtctacct caagcgcgag
     5701 gacctcaacc ataccggtgc gcacaagatc aataatgtga tcggccaggc actgctggca
     5761 cggcgcatgg gcaagccgcg cgtgatcgcc gagaccgggg ccggcatgca cggcgtggcg
     5821 gccgccacga tcgccgcgcg ctatggcatg gaatgcgtcg tctacatggg cacggaagat
     5881 gtcaagcgcc aggtgcagaa cgtctaccgc atgaagctgc tcggggcgac cgtcgtgccg
     5941 gtcgaaagcg gctcgaagac gctcaaggac gcgctgaacg aggcgatgcg cgactgggtg
     6001 accaacgtcg agagcacgtt ctacatcatc ggtaccgtgg ccggcccgca tccctacccg
     6061 atgatggtgc gtgatttcca gtcggtgatc ggacgcgaag ccctggaaca gatgcaggag
     6121 ctcgccggcc gccagcccga tgcggtcctc gcctgcgtcg gcggcgggtc caatgcgatg
     6181 gggattttcc acgcctacgt cccggacgag aaggtgcggc tgatcggcgt ggaggccgca
     6241 ggccacggcc tcgagagcgg caagcacgcg gcgacgctct gtacgggcaa gccgggcgtg
     6301 ctgcacggca accgcactta tctgctgcag gacgagaacg gccagattct cgagacccac
     6361 tcgatctcgg ccggcctcga ctatcccggc gtcggccccg agcatgcctg gctgaaggac
     6421 agcgggcgcg ccgagtacgt gtcggtcacc gacgaggagg cattgcaggc gttccacgac
     6481 ctgtgccgcc tggaaggcat catacccgcg ctcgaatcgg cgcacgcgct cgcctacgcc
     6541 gccaagctcg ctccgcgcat gaagaaggac cagatcctgc tcgtcaacct ctccggccgc
     6601 ggcgacaagg acatgcacac ggtggccgag agatcgggca tcaagattta aggaggaagg
     6661 atgaaagcgg aaggcagaag gcagaaggat tggtttgctg ttcgtctgtt acttcatcct
     6721 tcatccttct gccttgattg acatgtcgcg tattgcagcc accttcgagg cgctgaaacg
     6781 gcaggggagg aaagctctca tcccgttcat caccgccggc gaccccgacc cgaagctcac
     6841 cgtgccgctg atgcacgcgc tcgcgaaggc cggcgccgac gtgattgagc tcggcgtacc
     6901 gttctcggac cccatggccg acggcccggt gatccaacgc tcgagcgagc gggcgttgca
     6961 gcaccacgtc tcgctccggc aggtgctggg gcaggtcgcc gagttccgca ggaccgaccg
     7021 caccactccg gtggtggtat tcggttatgc aaacccgatc gaagcgctgg gcctggagcg
     7081 cttcgcggac gcggccaggg cggcggatgc cgacggcgtg ctggtcgtgg actacccgcc
     7141 cgaggaagcg cgactgctgg tcgagttgct cgatgcgcgc ggtctcgaca cgatattcct
     7201 gctgtcgccg accaccaccg acgagcggct caccgaggtc gcgaagctcg gccgcggata
     7261 cctgtattac gtttcgttga ggggcgtgac cggcgcctcc catattgaca tcgccgacgt
     7321 tgccgcccgc gtgaagcacg tacgcggcta cacgagattg ccggtgggcg tgggcttcgg
     7381 catccgcgac gcccagagcg cacgcgccat cggcgcggtc gccgatgcgg tcgtcatcgg
     7441 cagcgcgctg atccaggaaa tcgaaaaaac gccgcgggac aaggtgatgg cgcgggtcga
     7501 acagttcctg tcccctattc gcgaagcgct ggacagtctt tcggcggtga gggcatgagt
     7561 aagggccgcc gacatagcga ggcccatgtg atcaagacca tgtccgaggc ggccttatga
     7621 gctggctcca gaagctgctt ccgcccaaga tcaagcgcga caccggcagc ccccggaagg
     7681 tcgtgccgga ggggctgtgg agcaagtgcg aatcgtgcga ggccgtgctc taccgcgccg
     7741 acctcgagaa gaacctccac gtctgcccca agtgctcgca tcacaaccgc atctcggcgc
     7801 gcgagcggct cgactgcctg ctcgaccgag aagggcgcta cgagatcggc gcggaggtcg
     7861 tgccggtgga cagcctcaag ttcaaggaca gccggcgcta caccgagcgg ctggaggagg
     7921 cgcgcagcga gacctcggaa gaggacgcgc tggtggtgat gcagggatcg atcaagacca
     7981 ttccagtggt ggcggccgcc ttcgaattcg gcttcctcgg cggctcgatg ggctcggtgg
     8041 tgggcgagcg cttcgtgcgc ggcgtccagg tttgtctgga gcagcggctg cccttcctct
     8101 gcatcaccgc gagcggcggg gcgcgcatgc aggaggggtt gctctcgctc ctgcagatgg
     8161 cgaagacctg cgccacgctg acccagcttg cgcgcgagcg gctgccgttc ctcaccgtcc
     8221 tgaccgatcc cacgatgggc ggggtgtcgg cgagcttcgc catgatcggc gacgtcgtga
     8281 tcgctgagcc cggcgccctc atcgggtttg ccggcccgcg ggtcatcgag cagaccatgc
     8341 gcgagacgct gccggagggt ttccagcgct ccgagttcct gctcaagcac ggcgcgatcg
     8401 acatgatcgt ggaccgccgc gagatgcgcg acaagctcgc gagcctcgtc acgattctgc
     8461 tcaagctgcc acccgcggct tgagtgttcc cggtttccgg ttccaggctc caaattccaa
     8521 gttcaaagct caatgttccg ggttcgacgt tggattcccg aaccttgagc cttgaacttt
     8581 gagtcttgaa ccttgaacct tgaatctgaa cgtcgaacgc tccagcaatg gctcgagtat
     8641 atcgagcgct tgcatcccaa gcagatcgcg ctcgggctgg agcgggtcag ccgggtgaag
     8701 caggcgctcg ggctggaacc gcgcttcccg atcatcaccg tcggcggcac caacggcaag
     8761 ggctcggtgt gcgcgatgct ggaggcgatc ctgcatcacg ccggctactg catcggctgc
     8821 tacacctcgc cgcacctgct gcgctacaac gagcgggtgc gcgtcgcgcg cgccgaagcg
     8881 tccgacgccg acctcgtgcg cgcgttctcg gccgtggatt ccgcgcgggg gagcgtgacg
     8941 ctcacgtatt tcgagatggg aacgctcgct gcgatgcggc tgttcgccga gcgcggcgtc
     9001 gaagccgccg tgctggaagt ggggctcggc ggccggctcg acgcggtcaa cgccttcgat
     9061 gccgactgcg cggtgatcac cacggtggac atcgaccatg tggatttcct gggcggcgac
     9121 cgcgaggcga tcggttacga gaaggccggc atactgcgcg gcggccggcc ggccgtatgc
     9181 gccgaccccg cgccgcccgc gagccttatc cgccatgcgg cggagatcgg ggcgcaattg
     9241 ctgcggatcg gggccgactt cggcgccagc gcgcaaggca ggcaatggca gtactggggc
     9301 ccgcgcggca agcgcaacgc cctgccgtac cccgctctgc gcggcgcgtg ccagctcgcc
     9361 aacgcggcgg cggcggtcac cgcgatcgag tgcctgcgcg agcggttgcc ggttaccatg
     9421 aacgacatcc gcgccgggct gttacagacg gacatccccg gccgcttcca ggtcctgccg
     9481 gggcggccgg cggtcatact cgatgtcgcc cacaatccgc aggcggcgcg ggtgctggcg
     9541 gcgagcctcg atgcgatggg ccgctcgggc cgcacgctcg ccgtgttcgc gatgctgaat
     9601 gacaaggaca tcgccggcgt ggtcgccgcg ctgaaagcgc acgtcgctca ctggttcgtg
     9661 gccggtctgg gcgggccgcg cggggcgagg gttgcggagc ttgggcacat cctcgccgcc
     9721 gctgccatca gcgcggtcac gcgctgtgaa gatgtcgccg tcgcctacgc gcaagcctgt
     9781 gatatggcga cggaaaatga tagaattgta gtgttcggat cgttctacac cgtggccacg
     9841 gtgatgcagt tgcgagcgtc ccggcccggg gggtagagcc gagttggtcc gaagcccgtt
     9901 tctgcccggg ggcggcggaa ttaacggcgg cagatggcgc gaacgatcag cgacgaagag
     9961 ctgcagctta agcgacgcgc acggcggcgc ctgatcggcg ccatcgtgct ggtgaccgcc
    10021 atcgtagtag tgctgcccat ggtgcttgac tcggagccca agccagccag ccaggagatc
    10081 tccatccaga tcccttcgcc cgactcgggc gtcttcacct ccaaggtggt gccgcttcct
    10141 gcgtccccgg aggccaaacc tgcgccgaaa acgccgcagg gcaagccggc ggccgcgccg
    10201 cctgcggagg aagcgaaaaa ggtcgccgcc gcgccgccgc cggtcgcacc ggcagcaccg
    10261 gcgccaaagc aggtcgctcc tgaaccggcg gcgaaacccg agccttccaa accagacgac
    10321 gcagcccccg ccaagaagcc ggccaaggcc actgcgaaga ctgcgaagaa ggccaggccc
    10381 tcgcccaagc cggcagccgg gcggttcgtg gtgcaggtga tcgcgctcgc cgacgcggag
    10441 aaggcgaagc agatgcagga gaaaatcgca gcctcgggca tcaagtccta caccgaagtg
    10501 gtcaagaccg cgaaaggcga cgtgacgcga gtgcgggctg gcccgttcgc ctcgcgtgcg
    10561 gccgcggaaa aggcgcgcga gcagctgaaa acgctgggca tgaacggcaa catcacaacg
    10621 agatgaccct ttttgatatc gctgtgcttg tcatcatcgg attgtcggtt ctgctcagcg
    10681 tgatacgggg tctcatgcgc gaggtgctgg cgctcgccgc gtgggtggtg gcgttcctcg
    10741 ccgcgaatct gctcgccggg caggtcgcgc cgtggctgcc ggcggcgata ccgactgagg
    10801 agctgcggct gctggccggc ttcgtgggcg tgttcctggc ggtgctggtc gcgatgagcg
    10861 tgcttgccat cgtggcctct aaactggtaa aaagcgccgg tctcgggctg gaagaccgcc
    10921 tgctgggagg catgttcgga ctcgccaggg gcctgctgat agtggtgatc ctggtgttgc
    10981 tggcggggct gacctcgctg ccccgccagg cggtgtggcg caatgcggta ttgagcgatc
    11041 cgctggtggc gttcgccgga gaggtcaagc actggctgcc ggcggacctg gcgcaacgca
    11101 tcacttatga ctgagagcac acgatgtgcg ggatagtcgg ggtcgtcgcc aagaacccgg
    11161 tcaaccagct gctctatgac gggctgatgg tgctgcagca ccgcggccag gacgcggccg
    11221 gcatcgtcac cgccgagggc cccactttcc atatgcacaa agggcccggc atggtgcgcg
    11281 acgtgttccg cacccgcaac atgcgcgcgc tgacgggttt ctccggaatc gcccacacgc
    11341 gctaccccac tgcggggtcg gcgtggtcgg cggcggagtc gcagccgttc tacgtcaact
    11401 cgccgttcgg catcgtgctc ggtcacaacg gcaatctgac caacaccgag cagctcaagc
    11461 aggagctgtt ccgccaggac ctgcggcacg tcaacaccaa ttccgactcc gaagtgctgt
    11521 tgaatgtcct tgcgcacgag ctgcaggaga acgccaccaa ctacaagctc gaccccgcga
    11581 ccatcttcac ggcggcgtcc ggcgtctacc gccgctgccg tggcgcctac gccgtggtgg
    11641 cgatgatcgc cggctacggg ctgctcgcct tccgcgacgc ccacggcatc cggccgctcg
    11701 tcttcggccg cgccgaggcc ggccagggct acgagtacat ggtcgcctcc gagagcgtgg
    11761 cgctcgacgc gctcggcttc caggtggtgc gtgacgtcgc gccgggggag gcgatcttca
    11821 tagaccagga cggcactttc tacagccgcc agtgcgttcc gaacgccacg ctcaatccgt
    11881 gcatcttcga gttcgtctat ctcgcgcggc cggactcggt gatcgacggc atttcggtct
    11941 acgagacgcg gctgcggatg ggcgtaagcc tcgcggagaa gatccggcgc cagtaccgcc
    12001 acctcgacat tgacgtcgtg atcccgatcc cggactcgag ccggccggcg gcgatggagc
    12061 tctccaagca gttgagcctc ccgtaccgcg agggcttcat caagaaccgc tacatcggcc
    12121 gcaccttcat catgccgggg caggggatgc gccggaggtc cgtgcgccag aagctcaact
    12181 cgatcgcgat ggagttccgc ggcaagaacg tgctgctggt ggacgactcc atcgtacgcg
    12241 gtaataccag ccgggagatc gtccagatgg cgcgcgagtc cggcgcgcgc cgcgtgttct
    12301 tcgcctcggc cgcgccgccg gtgcgctatc ccaacgtcta cggcatcgac atgcccacgc
    12361 gcgaggagct gatcgcctgc ggacgcagcg aggccgagat cgcgcgcgag atcggctgcg
    12421 acgagctgat ctaccaggat ctggacgcgc tgcgggagga cgtgcgcagc gtgaatcccc
    12481 ggatcgtcaa cttcgaggac tcctgcttca gcggcgtcta cgtgacgggc gacgtgacgc
    12541 aggagtatct cgccggcgtg gaggctcagc ggcgcgacgg ggcaaaaatg gccgaaatgg
    12601 ccgcggcggc aagcgcccag ctcgacctcg gactcgaact ggtcgagtga gcgctgccgg
    12661 tgattcgaca agagcgtgaa tagtgattgg tgattggtga atggtgagct gaacccactc
    12721 gcaattcacg cttcgcggtt tgctccattc accattcact attcaccatt cacgccgttg
    12781 gatctgtaag ggtgacatgt tgacaattcc caagacgcag agtaattttc cttccgcgcc
    12841 gatttgaggg tcagcttgcg ggcgcgttac aagtacagct aaagcgacgg agaaagaacc
    12901 cgttcgcgga agctgaacgg gtttttttat gccttgagcg gacgaaagag cgtgacatat
    12961 gagcgaagac tatcaactgg ataccttggc ggtgcgggcg gggaccgagc gcagccagtt
    13021 cggcgagcac tcggaggcgc tctacctcac ctcgagcttc gtgttcaagt cggcggcgca
    13081 agctgccgcg cgcttctcga accaggagcc ggggaacatc tactcgcgct tcaccaatcc
    13141 gacggtgacc atgctcgagc agcggctcgc cgccatggaa ggcgcagagt gctgcgtcgc
    13201 cacgtcgtcc ggcatgtcgg cgatcctcgc gtgcgtgatg gggctgctca agagcggcga
    13261 tcacatcgtg tgctccgccg gcgtgttcgg cgcgacggtg cagctgctcg gcggcatcct
    13321 caagcgcttc ggcgtagaga ccaccttcgt gtccggttcc gacgccgacg cgtggcaaaa
    13381 ggcgatgcag gccaacaccc ggctgctgta cctggaaacc ccgtccaacc cgctgatgga
    13441 ggtctatgac atcgcggcgc tcgctaagat ttcgaaaaag gccagcgcgc tgctcgcggt
    13501 ggacaacgtc ttctgtacgc ccgcgttgca gcgcccgctc gaactcggcg ccgacatcgt
    13561 catccactcc gccaccaagt ttctcgacgg gcagggcagg gtgctgggcg gggcggtgct
    13621 gggcaggcgc gacacgatct acgagggcgt gttcggtttt cttcgcaccg cgggaccgac
    13681 gctctccccc ttcaacgcgt gggtgatact caaggggctg gaaacgctca agatccggct
    13741 ccaggcgcaa tcggcctccg cgctggagct tgcgcgctgg ctggagcaac agccgaagat
    13801 cgagcgcgtc tactacccgg ggctgccgtc gcatccccag catgagctgg cgaagaagca
    13861 gcagaaaggc tttggcgcga tcgtgtcttt cgacgtcaag ggcggcaggg acgccgcctg
    13921 gcgcgtcgtg gacgccaccc ggctgctgtc gattaccgcc aatctcggcg acacccgcac
    13981 caccatcacg caccccgctt ccaccaccca cgggcgcatc tcccaggaag cgcgtaatgc
    14041 cgcagggatc ggcgacggcc tgctgcgcgt cgcggtgggc ctggagagcg tcgaggacat
    14101 caaggcagat ttggcgcgtg gcctgagcgc gtagctttct gatgccggag tgctgcgatg
    14161 ggcgaagcgc aattccacgg tgtttttccc tacctggtct ctcccgtcga ggcgtccggc
    14221 gaggtcaaga gcgaggtcct tgagcggctg tgcaatgatc tgatcgaggc cggtgtccac
    14281 ggcctgacgc ccttgggctc gacaggcgag tttgcctacc tgtcgtggcc gcagaagaag
    14341 cggatcgtgg aagcggtggt cagcgcggca cgcgggcgcg tgccggtggt cgccggagtg
    14401 gcctcgacaa cgatcgcgga tgccgtattc caggcgcgtg aaatggagcg catcggctgc
    14461 aacggcatcc tcgccattct cgaggcttat ttcccggtta cggagaacgg cgtattcgag
    14521 tacttcaagg cggtcgcgga ggcggtttcc ctgccggtcg tgctttatac caacccgatt
    14581 ttccagcgct ccgacctgac cctgcccgtc atcgaccgcc tgagccggat cccgaatgtg
    14641 cgctatctca aggacgcgtc atacaacacg ggtcgtctcc tctcggtgat caaccgggtc
    14701 gaggggcggc tgaaagtgtt ctccgcttcg gcgcacattc ccgcttgcgt catgctgatc
    14761 gggggggtgg gatggatggc gggcccctcg tgcctggtgc cgcggcaaag cgtcaggttg
    14821 tacgaactgt gcctgcgcaa ggactgggat gccgcgatgc ggctccagca ggatctgtgg
    14881 aatctcaacg aggcgttcgc aaagcacaat cttgcagcct gcatcaaagg cgggctggaa
    14941 ttgcagggct accccgtcgg cgtcccgctg ccgccacag
//
LOCUS       DBNGWZ010000399         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2188617, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000399 DBNGWZ010000000
VERSION     DBNGWZ010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2188617"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="AC16CX_11865"
     CDS             complement(<1..1637)
                     /locus_tag="AC16CX_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR3013244.1"
                     /translation="MKTLLLVDGSSYLYRAFHALLRANLRSKKGEPTGAIYGVLNMLR
                     RLRKEIPADYIACVFDAKGKTFRDEIYPDYKAHRPPMPDDLAVQIAPLKEAIVALGWP
                     LLEVSGVEADDVIGTLAKHAESQGLRVIIASGDKDLTQLVNPVITIENADPKTGQREI
                     LDEAGVANKFGVKPERMVDYLTLVGDSVDNVPGVEKVGPKTAVKWLSQYGTLENVVRH
                     APEIEGVVGENLRKALDWLPRARELLTIKSDVELPVNVQELTARPRDAAKLAELFDRF
                     DFKSWRRELEEHAPTGTEQAQHSAAANGVAADAARAPQLAPDRNYETILTEKQLDAWI
                     GEIGKADLVAFDTETTSLDPMRAELVGLSFSVEAGAAAYVPLAHRYAGAPRQLEREAV
                     LARLRPWFEDAERAKLGQNAKYDMHVLANHGIRLAGFAHDTLLQSYVLESHRPHDMDS
                     LAERHLGVKTITYDEVTGKGASRIGFEQVAVERATEYAAEDADVTLQLHQALHPKIEA
                     DDKLARIYREVEMPLIAVLFNMERHGVLLDTALLRDLS"
     gene            1780..2478
                     /locus_tag="AC16CX_11870"
     CDS             1780..2478
                     /locus_tag="AC16CX_11870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3013245.1"
                     /translation="MSPELAEKRWSARESWRVFGIMSEFVEATERLSAIRPAVSFFGS
                     SRILPGHAYYALAESIARQLSDAGFSVVSGGGPGIMEAANKGAYFGKSPSVGLNIQLP
                     HEQASNAYQDISQTFRHFFARKVMFVKFATAWVVLPGGFGTLDELMEALTLVQTGKTR
                     RMPIILVHEPFWRGLLDWFRRMLVAEGMIDAADVDLIQVHNEPQAVVEAIFNYYEKRG
                     FEPSAAEREILLNL"
     gene            2621..2965
                     /locus_tag="AC16CX_11875"
     CDS             2621..2965
                     /locus_tag="AC16CX_11875"
                     /inference="COORDINATES: protein motif:HMM:NF022639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HIR3013246.1"
                     /translation="MNARLLLAALLLAIALPVAAQQSKPPALQPVPEPPPPPPGYELD
                     PALEPQVTIIKRGTDTVEEHRIGGKLYMIKITPARGAPYYLVDHRGDGTFVRQDNYDS
                     GIRPPMWVIFSF"
     gene            3053..>3660
                     /locus_tag="AC16CX_11880"
     CDS             3053..>3660
                     /locus_tag="AC16CX_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR3013247.1"
                     /translation="MSVFTPVSRKQLSAWLKNYSIGTLIAFEGIPAGIENTNYFVTTA
                     QGRFVLTLFEKLKARELPFYLRLMAHLSGHGIPCPRPIASRKGRLLGRLNGKPAALVS
                     FLPGREVAAPTAGQCAQAGAMLANIHVAGASYAGRMDNPRGPKWWTSVLPRIVPFLDA
                     EDAALLRGEARFQSRFRLRGLPRGAIHADLFRDNVLFEDGRIS"
BASE COUNT          632 a         1203 c         1128 g          697 t
ORIGIN      
        1 gaaagatccc tcaggagcgc ggtgtcgagc agcacgccgt ggcgctccat gttgaagagc
       61 accgcgatca gcggcatctc cacctcccgg tagatgcgcg cgagcttgtc gtcggcctcg
      121 atcttagggt gcagtgcctg gtgcaattgg agcgtcacgt cggcatcctc ggccgcgtac
      181 tctgtggcgc gctcaaccgc cacctgctcg aaaccgatcc ggctagcgcc cttgccggtg
      241 acttcgtcgt aggtgatggt cttcacgccg agatggcgct ctgcgaggct gtccatgtcg
      301 tggggccgat ggctctccag cacgtaggac tgcagcagcg tgtcgtgcgc gaagccggcg
      361 agccggatgc cgtggttggc gagcacgtgc atgtcgtatt tcgcgttctg gccgagcttg
      421 gcgcgctccg cgtcctcgaa ccagggccgg agccgcgcga gcaccgcttc gcgctcgagc
      481 tgccgcggcg cgccagcgta acggtgcgcg agcggcacgt acgcggcggc gcccgcttcc
      541 accgaaaacg acaagcccac cagctccgcc cgcattggat cgaggctggt ggtttcggta
      601 tcgaacgcga caagatcggc cttgccgatc tcgccaatcc aggcgtccaa ctgtttctct
      661 gtgagtattg tttcgtagtt tcggtcaggc gcgagttgag gggcgcgcgc cgcatcggca
      721 gccacgccgt tggcggcggc tgaatgctgt gcctgctccg tgccggttgg cgcgtgctcc
      781 tccaactcgc gcctccagga tttgaaatcg aagcggtcaa acagctcggc gagcttcgcc
      841 gcgtcgcgcg gccgcgcggt gagctcctgg acgttcaccg gcaactcgac gtcgctcttg
      901 atggtgagca gttcgcgtgc ccgcggcagc cagtcgagcg ctttgcgcag gttttccccg
      961 actactccct cgatttctgg tgcgtgccta acgacattct caagtgtccc gtactgggaa
     1021 agccacttta cggcggtctt aggaccgact ttctcaacgc ccggcacgtt gtccacgcta
     1081 tctccgacga gcgtaaggta gtcgaccatt cgttcgggct tgacgccaaa cttgttcgca
     1141 acgccagctt catcaagaat ctcgcgttgg ccggtcttgg gatcggcgtt ctcgatggta
     1201 atgacaggat tgaccagttg cgtaagatcc ttgtcgccgc tggcaatgat cacgcgcagc
     1261 ccttggctct cagcatgctt ggcgagcgtg ccgatcacgt catcggcctc cacgccgctc
     1321 acctcgagca ggggccagcc gagcgccacg atcgcctcct tgagcggcgc gatctgcacg
     1381 gccagatcgt cgggcatggg cgggcgatgg gccttgtagt cgggatagat ctcgtcgcgg
     1441 aaggtcttgc cttttgcgtc gaatacgcag gcgatataat cggcggggat ttccttgcgc
     1501 agccgacgca acatgttgag cacgccgtag atcgcgccgg tcggctcgcc ttttttactg
     1561 cgcaggttgg cacgcagcag cgcatggaac gcgcggtaga gataggatga gccgtcaacc
     1621 agcagcagcg tcttcatttc aaaagccgga gaggtgtaag gagtgagggg tgaggggaag
     1681 aaccttcact tgcagccacc tcgcactcaa ccccttactc ctcactcttc actcctcact
     1741 ggggtcaaaa tgacagagaa agacaagctc gccaacgcca tgtcacccga gctcgccgaa
     1801 aaaagatggt cggcgcgcga atcgtggcgg gttttcggca ttatgtcaga attcgtcgag
     1861 gcgacggaac gcctgagcgc gatccgtccc gcggtgagct tcttcggcag ctcccgcatc
     1921 ctgcccgggc acgcctacta cgcccttgcc gagagcatcg cccgccagct ctccgacgcc
     1981 ggcttcagcg tcgtctccgg cggcggcccc ggcatcatgg aggcggcgaa caagggcgcc
     2041 tatttcggaa aatcccccag cgtcgggctc aacatccagc tgccgcacga gcaggcttcc
     2101 aacgcctacc aggacatcag ccagaccttc cgtcacttct tcgcccgcaa ggtgatgttc
     2161 gtcaagttcg caaccgcgtg ggtggtgctg cccggcggct ttggcacgct cgacgagctg
     2221 atggaagcgc tcaccctggt gcagaccggc aagacccgca ggatgccgat catcctggtg
     2281 cacgagccgt tctggcgcgg cctgctcgac tggttccgcc gcatgctggt cgcggagggc
     2341 atgatcgacg ccgcggacgt ggacctcatc caggtccaca acgagccgca ggcggtggtg
     2401 gaggcgatct tcaactacta tgagaagcgc ggtttcgagc cttccgcggc ggagcgtgag
     2461 atattgctca atttatagga ttgaggaatg agccatcagg attgagccgg cccgggcaaa
     2521 acccgaatct tgctcaatcc tcagtcctca atcctccgcc ctcgatcctg cttttaataa
     2581 cagcgtgaaa ctccgataat agcgccgggt ctaataagac atgaacgctc gcctgcttct
     2641 cgccgccctg ctcctggcca tcgcgctccc ggtggcggcc cagcagtcca agccgcccgc
     2701 tctgcagccg gtgccggagc cgccgccccc gccgccgggc tacgagcttg atcccgcgct
     2761 ggagccgcag gtgaccatca tcaagcgcgg caccgatacc gtcgaggaac atcgcatcgg
     2821 cggcaagctc tacatgatca agatcacgcc ggcgcgcggt gcgccctatt acctcgtgga
     2881 tcaccggggc gacggcacgt tcgtgcgcca ggataattac gacagcggca tccggccgcc
     2941 gatgtgggtg atcttcagct tctagcattc agctgtcagc attcagcttg tttgtaacga
     3001 agccgtcatg gtttaagctg gcggctgact gctgatggct gacagctaga acatgtccgt
     3061 tttcaccccc gtctcccgca aacagctcag cgcctggctc aagaactact ccatcggcac
     3121 cctcatcgct ttcgagggca tacccgccgg gatcgagaac accaactact tcgtcaccac
     3181 cgcgcagggc cgcttcgtcc tcaccctgtt cgagaagctc aaggcccggg aactgccgtt
     3241 ctatctgcgg ctgatggccc acctgtcggg ccacggcata ccttgtccgc ggccgatcgc
     3301 aagccgcaag ggcaggctgc tcggccgcct caacggcaag ccggccgccc tggtgagctt
     3361 tctccccggc agggaggtcg ccgcgcccac cgccgggcag tgcgcccagg ccggggcgat
     3421 gctcgccaat atccacgtgg ccggcgcttc gtacgccgga agaatggaca atccgcgcgg
     3481 gccgaagtgg tggacgtcgg tgctgccccg gatcgtgccg ttcctcgacg cggaggacgc
     3541 cgcgctgttg cgcggggagg cgcgattcca gtcccgattc cgcctccggg gattgccgcg
     3601 cggagcgatc cacgccgacc tgttccggga caacgtgctg ttcgaggacg ggcgcatctc
//
LOCUS       DBNGWZ010000400         3261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1056134, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000400 DBNGWZ010000000
VERSION     DBNGWZ010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1056134"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1252
                     /locus_tag="AC16CX_11885"
     CDS             335..1252
                     /locus_tag="AC16CX_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3013248.1"
                     /translation="MSRGEVRRGWFVAGLLLAIGGVVFFSLRPIFIKLAYAYVVDPVT
                     LIALRMVFSAPFFVGAAVWVRSGSEHRPLAGRDLALVAGLGLLGYYAASFLDFLALQY
                     IPAGLGRLLLFTYPTIVVILSALLIDKRVTGREIAALALTYAGLALVLAHSLEGEVAN
                     VWLGAALAFASSVCYALYLVAGAEVIMRVGSVRFSAYAMSAASAACILQFLLLRPLSA
                     LDLPAPVYLYAVAMAVVSTAIPVFMTSEALRRIGANTVAIIGALGPVTTIAFGWLGLE
                     ERMTPLQLAGSALVLVGVVVISLRPAQPA"
     gene            complement(1320..2087)
                     /locus_tag="AC16CX_11890"
     CDS             complement(1320..2087)
                     /locus_tag="AC16CX_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3013249.1"
                     /translation="MDAAPSLVEVAALVGDTARATMLSALMGGQSLTAKELAYYANVS
                     RSTASGHLSKLVAARLLTVIRERRFSYYRIASPLVASMLESIKVVAAIEVPPRHQPRS
                     ANDDALRFARSCYDHLAGQVGVAVTDALVAMGHIVLTDEGGEVTRSGSRFLSAFGADL
                     TPRTRRIFCQPCLDWSERRYHLRGLVGARILDRLLELGWLKCVSGTRALKLTPSGSAG
                     LSEIFQIGINKEGVSTSSPMERTDSWGSPRVRSGYVA"
     gene            2257..2550
                     /locus_tag="AC16CX_11895"
     CDS             2257..2550
                     /locus_tag="AC16CX_11895"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3013250.1"
                     /translation="MVAYWVARAKVNNPDQYKKYTDLIPGILQKFGGKILARGGKFKI
                     VEGPEKFHRFVVIEFPSFEKAVACHESAEYLEAAAHRKKDGASELEIVIVESI"
     gene            2594..>3261
                     /locus_tag="AC16CX_11900"
     CDS             2594..>3261
                     /locus_tag="AC16CX_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3013251.1"
                     /translation="MGMAAVIYKKGAPDNFVWEEIKVGSPERGQVRLRSTAVGVNFAD
                     TYHRAGIPHPMIVGDPPVVLGFEGVGEIEELGLGVTGFSIGERVCTCLPPLGAYSQER
                     LYPADKLIKIPKDLPLDDVQLAGLILKGMTAQYLLHRTHKVQPGDYVLIHAAAGGMGH
                     FLCPWARHLGATVIGTVSTDAKAEIARNLGCHHVINYSTEDFVAVTRKITDGKGVDVV
                     YESIG"
BASE COUNT          641 a          984 c         1011 g          625 t
ORIGIN      
        1 ccaacccaac gtgaacgcaa acatatgctg gccgacgacc tgcgcgacca gggccagcca
       61 gtaaaggcga aagtcgcgca ctctcagcgc gaggagggga ttggctttat cttccacgaa
      121 tgatgcgttt ttggttgtat cccaagctta ccgcaccatt gaaaccggga gtgcgggaga
      181 tcgggagagc gggagcggga agagccgggt tcgatctcac gatctaccga cctcccgagc
      241 cacggagccg gatagagagc ctcacgggta caattgccgt cattccaatg ggtaaacagc
      301 gtacttcagc aggcatgacc gggcggtgcc tgccttgagc cgcggcgaag taaggcgtgg
      361 ctggttcgtc gccgggctgc tgctcgccat cggcggggtc gtgttcttct cgctgcggcc
      421 gatcttcatc aagctcgcgt atgcttacgt cgtcgacccg gtcacgctga tcgcgctgcg
      481 catggtgttt tcggcgccgt tcttcgtcgg ggcggcggtg tgggtgcgca gcggctcgga
      541 gcaccggccg ctcgcggggc gggacctggc cttagtcgcc gggctcggac tcctgggcta
      601 ctacgcggcg agcttcctcg acttcctcgc ccttcagtac attccggcgg gactcgggcg
      661 gctgctgctc ttcacctacc ccaccatcgt ggtcatcctg tcggcgctgt tgatagacaa
      721 gcgcgtgacc ggccgcgaaa tcgcggcgct cgccctcacg tatgccgggc ttgcgctcgt
      781 actcgcgcac tcgctggagg gggaagtcgc caacgtctgg ctcggcgcgg cgctcgcgtt
      841 cgcgagctcc gtctgctacg ccctctacct cgtcgccggc gccgaagtca tcatgcgcgt
      901 cggctcggtg cgcttttccg cctacgcgat gagcgcggca agcgccgcgt gcatcctgca
      961 attcctgctg ctgcgcccgc tctcagcttt ggatctgccg gcaccggttt acctctacgc
     1021 ggtcgcgatg gcggtggtga gcacggcgat accggtgttc atgacctcgg aggcgctgcg
     1081 ccggatcggc gccaacaccg tcgcgatcat cggcgcgctc ggccccgtca ccacgatcgc
     1141 gttcggctgg ctcgggctgg aggaaagaat gacgccgctg caactcgcgg gttccgccct
     1201 cgtgttggtt ggcgtggtgg tgatcagcct aaggccggcg cagccagcct aacaggcact
     1261 gtccgcttgc ggccagaacc ggacattcga ctacggcaag aaatgggcaa cgatatgggc
     1321 taagcaacgt agcctgaacg cactcggggc gaaccccatg agtccgtgcg ctccattgga
     1381 ctcgatgtcg acacaccctc cttgttgatc ccgatctgga agatttcgga caagccggcc
     1441 gatccagacg gcgttagttt gagtgcgcgg gtgcccgaca cacatttgag ccatccgagc
     1501 tccaatagac gatccagaat ccgagctccg acgagtcccc tgaggtgata acgacgttcg
     1561 ctccagtcga gacaaggctg gcagaaaatc cgccgagtcc gcggcgtcag atcagcgccg
     1621 aacgcggaaa ggaaacggct gccagaacgc gtgacctcac cgccttcatc ggtgagcacg
     1681 atgtgcccca tggccacaag tgcatcggta acagcgactc ctacctggcc agccaagtga
     1741 tcgtagcagc ttcgggcaaa acggagcgca tcgtcgtttg ctgaccgcgg ctgatggcgg
     1801 ggcggcacct ctatggcggc gacgaccttg atgctctcca gcatgctcgc gaccagcggg
     1861 gacgcgatcc ggtaatagct gaagcggcgc tcgcgaatga cagtcaaaag ccgcgcggca
     1921 accagcttgc taaggtggcc gctcgccgtc gatcgagaga cattggcgta gtaagcaagc
     1981 tctttagccg tcagcgactg gccgcccata agggcgctaa gcatcgtagc tcgcgcagtg
     2041 tcacccacga gggcggccac ttccaccaga cttggtgccg cgtccatata taacccctat
     2101 tgccgataca ccctatcgct gataccggaa gcatgtcaat cgaaaacagt tcggcatgga
     2161 ccgaactatt cgagcgagac aattgcgcac gtcgcagcaa caatgcgctg tacccctagt
     2221 ctagcaaggg tgcacacggt tggcatggag gatgagatgg tcgcctattg ggtcgcgcgc
     2281 gcgaaggtca acaatcccga tcagtacaag aagtacaccg acttgatacc aggcatcctc
     2341 caaaagttcg gcggcaagat cctggcgcgc ggcggcaagt tcaagatcgt cgagggcccg
     2401 gagaaattcc accgcttcgt cgtcatcgag ttcccgagct tcgagaaggc ggtcgcgtgc
     2461 cacgaatcgg ccgagtacct tgaggccgcg gcgcaccgca agaaggacgg cgcgagcgag
     2521 ttggagatcg taatcgtcga gagcatctga gatccaccga tgtgacgatg tttaacagca
     2581 ggaggaacgc atcatgggaa tggcggcggt aatttacaag aaaggtgcac ccgacaactt
     2641 cgtatgggag gagatcaagg tcgggtcacc cgagcgtggc caggtacgtc ttcgtagcac
     2701 ggccgtcggc gtgaatttcg ctgacacgta ccatcgagcg ggtatcccgc acccgatgat
     2761 cgtcggcgat ccgccggttg ttctcggttt cgaaggagtt ggcgagatcg aggagttggg
     2821 tcttggcgtc accgggttct ccatcggtga acgagtttgc acctgcctcc ccccactcgg
     2881 cgcctacagt caggagcgcc tttacccggc tgacaagttg atcaagattc caaaggactt
     2941 gccattggac gacgtgcaac tggcaggact aatcctgaag ggaatgaccg cgcaatatct
     3001 cctgcatcga acgcacaagg tgcagccggg agattatgtt ctgatccatg cggctgctgg
     3061 cggcatgggc catttccttt gtccttgggc gcggcatctc ggagccactg tgattggtac
     3121 cgttagtacc gacgcgaagg ccgagatcgc tcgaaatctc ggttgtcatc acgttatcaa
     3181 ttactcgacc gaagacttcg tagctgtcac ccgtaagatt acggacggaa aaggcgtaga
     3241 cgttgtctat gagtcgatcg g
//
LOCUS       DBNGWZ010000401         2594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2566460, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000401 DBNGWZ010000000
VERSION     DBNGWZ010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2566460"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1927)
                     /locus_tag="AC16CX_11905"
     CDS             complement(374..1927)
                     /locus_tag="AC16CX_11905"
                     /inference="COORDINATES: protein motif:HMM:NF025126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3013252.1"
                     /translation="MPRYKAYSYEQGKLLPVAFERQILPGTFEYTLNHLIDHDVDLSA
                     FDGHYQNEDTGAPAYDPRILLKMVLFAYSRGLFTSRRIEQACRENVVFMALSADTCPH
                     WTTIADFISTRPEAIARLFREVLLVCDTLGLLGKELFAIDGCKLSSNAAKEWSGTHAD
                     LQKKQQKIEQAIQVMLDRHRDEDVRGGEAPGRERDAQYLTTVRRQVTKIKAFLATQPE
                     NVSPKGTIRQSNITDPESAKMKTAHGVLQGYTGVAAVDAKHQVIVHAEAHGEGQEHGL
                     LQPTVDAVRATFRAIAHDDNIFTTAKLTADAGYHTEANMHYVVEEGIDAYIADRAMRQ
                     RDPRFADADRYKVRHRRERRRVVAPRPRLFKPADFDHDPTKQTCVCPAGKRLYRNGSN
                     ITIRGFTGVKFTAPQSACLPGPLRAQCLKDPSRTKVRQVVFFTGRSPTAPETFTAKMQ
                     RKIDSVLGRLIYRQRLATAEPPFAHLRHAKGLHRFTVRGKTKVNAQWRLYCLVHNLFK
                     VHQFGPGYT"
     gene            complement(2020..2220)
                     /locus_tag="AC16CX_11910"
     CDS             complement(2020..2220)
                     /locus_tag="AC16CX_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013253.1"
                     /translation="MSAGQSMTRRLFVRTIIGLLLIMTWIGASAQPARADLTGNVNVF
                     LGAKGLDSLRSLGLCAAAQFWR"
     gene            complement(2381..>2594)
                     /locus_tag="AC16CX_11915"
     CDS             complement(2381..>2594)
                     /locus_tag="AC16CX_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013254.1"
                     /translation="TEGLQDVTADAIVNGVIVEALGVAEIEIPAKAKVVLRFTAEKPG
                     EFRVFCGAEQPKSHLKEGMSATLILR"
BASE COUNT          442 a          811 c          822 g          519 t
ORIGIN      
        1 tcgaatgaga cattccattt tctggattct tttgtttcaa ctatgcgatt cgctccagcg
       61 atgagccggt cgaccggact ggaagacacc aagtatggcg tctttggcgc accgcgtgat
      121 gttctacccg gtaggtccaa ttgcaactcg caggagcgac tcgcaaacta gatacctaac
      181 ctgaacgaac tgtgatcgcc aagcaatcgc ataagtcagc taacgagtct gtagaaaaac
      241 cccatcgcaa tgccgtccca cgaggcgaac cggtcgagcg gcaggccggt tcgccggtgt
      301 cgtcaccgct tcgtgccggt cttgccggtt gtcctcattg ccccgtgact ggcggccagg
      361 ccatgtggct tcatcatgta tatcccggcc cgaactggtg gaccttgaac aggttgtgca
      421 ccaggcaata gagccgccac tgggcgttga ccttggtctt cccgcgcacc gtgaaccggt
      481 gcagcccttt cgcatggcgc aggtgcgcaa acgggggctc ggcggtcgcg agccgctgcc
      541 ggtaaatcag tcgccccagc acggaatcga tcttgcgctg catcttcgcg gtgaaggtct
      601 cgggggccgt gggacttcgg ccggtgaaga acaccacctg ccggactttg gtgcgactcg
      661 ggtctttcag gcactgcgcc cggagtgggc ccggcaggca cgccgattgc ggggcggtga
      721 acttcacccc ggtgaaaccc cggatcgtga tgttcgaacc attccgatat aagcgttttc
      781 cggcgggaca cacgcaggtc tgcttcgtgg gatcgtggtc gaagtccgcc ggtttgaaga
      841 gacgcggccg gggtgcgacg acccggcgtc gttcccggcg gtggcgcacc ttgtagcggt
      901 ccgcatcggc aaagcgcgga tcgcgctggc gcatcgcccg gtcggcgata taggcgtcga
      961 tgccttcctc gacgacgtag tgcatgttgg cttcggtgtg gtagccggca tcggcggtga
     1021 gcttcgccgt cgtgaagata ttgtcgtcgt gggcgatcgc tcggaacgtc gcgcgcaccg
     1081 cgtcgaccgt cggctggagc agcccgtgtt cctgcccctc gccgtgggcc tcggcatgca
     1141 cgatcacctg atgcttggca tccaccgccg ccacgccggt gtagccttgc agcaccccgt
     1201 gagccgtttt catcttggcg ctctcggggt cggtgatgtt gctctggcgg atggtgccct
     1261 tggggctgac attctccggc tgggtggcca gaaaggcctt gatcttggtc acctgccggc
     1321 gcaccgtggt gagatactgc gcgtcacgct cccgaccggg cgcctcgccc ccgcggacat
     1381 cttcgtcgcg gtggcggtcg agcatcacct ggatcgcctg ctcgattttc tgctgcttct
     1441 tctggaggtc ggcatgggtg ccgctccatt ccttggcggc gttcgaggag agcttgcacc
     1501 catcgatcgc gaagagctcc ttgcccagga ggcccagggt gtcgcagacc agcagcacct
     1561 cccggaacaa ccgcgcgatg gcctcgggcc gggtactgat gaagtccgcg atcgtggtcc
     1621 agtgcgggca ggtatcggcc gacaacgcca tgaacaccac gttctcccgg cacgcctgtt
     1681 cgatccgccg gctggtgaag agcccgcgtg agtaggcgaa cagcaccatc ttcaagagaa
     1741 tgcgcggatc gtaggccggc gcgcccgtgt cctcgttctg gtagtgccca tcaaacgcgg
     1801 acaggtccac gtcgtggtcg atgagatggt tgagggtgta ttcgaaggtg ccggggagga
     1861 tctggcgctc gaaggcgacc gggagcaact tgccttgctc gtaggagtac gccttgtatc
     1921 tcggcatccg cacctccttc gccgcctgag cggtctgcgt ggtgtggggg caccagcccg
     1981 atgcggcatt atagcggttt tcggactttt tctacagact caacgccaga actgagccgc
     2041 cgcgcacaga ccaagcgagc gaagcgaatc aaggcccttt gcgccgagaa agacgttcac
     2101 gttacccgtc aagtcggccc gggcaggctg cgcagaagca ccgatccacg tcatgatcag
     2161 caagaggccg atgatcgtcc tgacgaataa tcgcctcgtc atcgattgcc cggcggacat
     2221 gagggacctc ctcggtcagt gcgtcgagtc acggtgaacc atctgacgct gaacggtggg
     2281 aagcggcgcg agcctagcac gcgcatcgat tgtcggtcaa tctccaacct gccgcccttg
     2341 cgcggaagac gctgcactgg gaacttcgcc tcccctcggt ctatcggagg atcaacgtgg
     2401 cgctcatgcc ttctttgagg tgcgatttgg gctgctccgc cccgcagaac acgcgaaact
     2461 ccccgggctt ttcggccgtg aaacgcaaga ccactttggc cttggcggga atttcgatct
     2521 cggccacgcc cagcgcctcg acgatcacgc cgttgacgat cgcatccgcg gtgacgtcct
     2581 gaagcccctc ggtg
//
LOCUS       DBNGWZ010000402         1535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2063911, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000402 DBNGWZ010000000
VERSION     DBNGWZ010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2063911"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..625
                     /locus_tag="AC16CX_11920"
     CDS             41..625
                     /locus_tag="AC16CX_11920"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3013255.1"
                     /translation="MFTGIVTAVGTIREARPARGDRRLVINGGSLDLRDVAIGDSIAV
                     NGACLTVVARKSRAFEVDVSRETLACTAGFDAGSRVNLEKALRLADRLGGHLVTGHVD
                     GVGTVKRIERAGSNRVMAVKAPAALAKYIARKGSIAVNGVSLTVNTVKGPEFAVNLIP
                     YTLASTTLGELRAGDKVNLEVDMLARYIERLKNR"
     gene            782..>1535
                     /gene="ribB"
                     /locus_tag="AC16CX_11925"
     CDS             782..>1535
                     /gene="ribB"
                     /locus_tag="AC16CX_11925"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR3013256.1"
                     /translation="MVVLVDEAERENEGDLLIAAEFVTPESINFMARHGRGLVCLTLT
                     EEKCRQLHLPLMVVNNRSPLGTNFTVSIEAAEGVTTGISAADRARTVQVAIRPDASAA
                     DLVQPGHIFPLMAQDGGVLVRAGHTEAGCDLARLAGLIPAAVICEILKDNGEMARLPD
                     LIEFATQHKLRIGTIVDLIEHRSRTESLVTRVAEREVETVHGKFRLYVYSDRIARETH
                     LALAKGRPAPGTEALVRVHEPVSVIDLLDTASR"
BASE COUNT          287 a          471 c          535 g          242 t
ORIGIN      
        1 ggatcgggcc ggatatccgc atcgtggcga ggttcgtata atgttcacgg gcatcgtcac
       61 ggcagtgggc acgatccgcg aggcgagacc ggcccgaggg gaccgaaggc tcgtcataaa
      121 cggcggcagc ctcgatctcc gcgatgtcgc gatcggcgac agcattgcgg tgaacggcgc
      181 ctgcctcacc gtggtggccc ggaagtcgcg ggccttcgag gtggacgttt cgcgggaaac
      241 actcgcttgc accgcgggtt tcgacgccgg cagccgcgtc aacctggaga aagcgctgcg
      301 tctggccgac cgcctcggcg gtcatctggt gaccgggcac gtggatggcg tggggacggt
      361 gaagcggatc gagcgggcgg gaagcaaccg tgtgatggcg gtgaaggcgc ccgcggcgct
      421 tgcgaagtac atcgcgcgca agggctcgat cgcggtcaac ggcgtgagcc tgaccgtcaa
      481 cacggtgaag ggaccggaat ttgcagtcaa cctgatcccg tacacgctcg cctcaaccac
      541 gctcggcgag ctgcgcgcgg gcgacaaggt caacctagaa gtggacatgc ttgcacgcta
      601 tattgagcgc ctcaagaacc ggtaatggat aatgagtaat gggtaatgcg cgaaaaccgc
      661 cggcacgaat aggggttcac ccatcactca tcactcatca cccatcacga ttatgacttc
      721 gagaccggaa acgggcgtga gcccgatcac cgacatcatc gccgacttca aggccgggcg
      781 gatggtggtg ctggtggacg aggcggaacg cgagaacgag ggcgatctcc tgattgccgc
      841 ggaattcgtc acgcccgaat ccatcaattt catggcgcgc cacgggcgcg ggctggtctg
      901 cctgacgctg accgaagaga aatgccggca gttgcatctg ccgctgatgg ttgttaacaa
      961 ccgctcgccg ctcggcacca atttcaccgt ctcgatcgag gcggcggaag gcgtcaccac
     1021 gggcatttcc gcggcggacc gcgcgcgcac cgtccaggtc gcgatccggc cggacgccag
     1081 tgccgccgac ctggtgcagc cggggcacat cttcccgctg atggcccagg acggcggcgt
     1141 gctggtgcgc gcgggccata ccgaggcggg ctgcgacctg gcgcggctgg cggggctcat
     1201 tccagccgcg gtgatctgcg agatcctcaa ggacaacggg gagatggcgc ggctccccga
     1261 cttgatcgaa ttcgccacgc agcacaagtt gaggatcggc accatcgtcg atctcatcga
     1321 gcatcgcagc cgtaccgagt cgctggtgac gcgggtggcg gagcgcgagg tcgagaccgt
     1381 gcacgggaag ttccggctct acgtttacag cgaccggatc gcgcgggaga cgcatctcgc
     1441 tctggcgaag ggcaggccgg ccccgggaac ggaagccctg gtgcgcgtgc acgagccggt
     1501 gtcggtgatc gacctgctcg acacggcgag ccgca
//
LOCUS       DBNGWZ010000403         9440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1687291, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000403 DBNGWZ010000000
VERSION     DBNGWZ010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1687291"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16CX_11930"
     CDS             <1..513
                     /locus_tag="AC16CX_11930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3013257.1"
                     /translation="FFEGVPPPGKEVPPEPVVRQPRRPPFTPPAKPVPPPAVPEKPPT
                     PDWTALFQKLPKVADGSVEWVRALNEKLIAPRPGIAPEAKDEQPVIDLNVELIPRGQP
                     AFKVIFPHKAHTQWLACANCHPAIFQMQRGADPITMAKVFAGEYCGRCHGKVAFALTG
                     CARCHAAMPK"
     gene            513..836
                     /locus_tag="AC16CX_11935"
     CDS             513..836
                     /locus_tag="AC16CX_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3013258.1"
                     /translation="MRSSLALAVALLLALLPARYGEAEVGDIPFTRKAAGTEDFPPAV
                     FPHWLHRMQFKCHVCHENVFQMKAGTNPVTMDAIYQGKYCGACHNGKTAFAPMFEHCS
                     RCHRQ"
     gene            863..2854
                     /locus_tag="AC16CX_11940"
     CDS             863..2854
                     /locus_tag="AC16CX_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013259.1"
                     /translation="MGMMIVFILLRPAEIPAQELWGLRLGTWGGEVQLGYGSDRQQAR
                     TEGGVTSDFAHQRYREQLTIRNQGSYFVDPRLVTGNLALTFGLFQDQERSDGSLNSRQ
                     GKLIGYATDFTFLAEKPYSGTLYANRTQNFATQPFGGHTDLTYENHGAVLRLREDSIL
                     RDWGVPYFSSNLRVYQENITESTTSLGQTFQRNELRNVLSVDGHKGFETSDLDLRYEF
                     TDHNDAAFPLGAFQTQMASLSYSLDFGPTLNRRWDSRLNYYSRTGLSPTTFLTADEHV
                     RIDHFKNLSTDYRYLLTRIDTQAGTTTTHNGIFHLQHRLYRNLTTNALLSGLRQELPD
                     GVVTGYAGQLDFNYRRGLPRDGRLFASAGGRYQLNDNDLGASQINVIDAAFSAPSPLG
                     GGAGFLLEQPFVIASTIVVVDTRGGARLPTTLGMDYDIVPEGNLIRIVPLPASLVIQP
                     GDPLAVSYSYRVDRSLKYTTASWWLRSGVDFRWIAFSAGHEQTDQTALSGGSSQFLEN
                     RRNDTAQLDLRGAWKMAEGQVGAAYQRYNATFLAYTRQRFNQHASYRPGNNFALAFNA
                     EESHTDFTLPLRQSDTRSAQLTTTWLAPGGWWTTALLGRRVYKDSLSPTETVNEAKAQ
                     ARKTYGKLMINSAFTRGDRVRGAFETDFWRVDVTITRRF"
     gene            2860..3912
                     /locus_tag="AC16CX_11945"
     CDS             2860..3912
                     /locus_tag="AC16CX_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3013260.1"
                     /translation="MMRNFPLLLMTLVTALSMSGCETPPSASVTKEVRAGLPVWPTPP
                     DEPRIRYVRSVSGPEDFGIARSFFQRLIDVFSGAGAVHFIRPTGVAEHDAALYVADPG
                     ARALWILDPVKNRFVKVEKVGELALVSPVAVAVRADGAVFLADTGLKKVFLLDREGRF
                     IRIAAEQGLSRPAGLAYDAAGERLYVADSIHHWIGIYDLNGALIRTWGERGTRNGEFN
                     SPTHLALDQTGTLLVTDALNFRIQAFDREGRFQWKLGRHGDGSGDFAAPKGLAADSEG
                     HVYVVDALFDAVQIFERDGTLLLAFGGQGIQAGQFWLPGGIFITAQDKIYVADAYNQR
                     VQVFVFGLNAGKETNR"
     gene            3996..5657
                     /locus_tag="AC16CX_11950"
     CDS             3996..5657
                     /locus_tag="AC16CX_11950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3013261.1"
                     /translation="MPLLVALAGFLALSTPTRAQTGISNTKHNLTTKVSQPTGVCVFC
                     HTPHNANPTRALWNRALPGVTYQLYASSTLKAQLDQPTGTSRLCLSCHDGILALGDLR
                     VPPPGGPLALGPLTGETVLGTDLSDDHPISFTYDSTLALNRGGLADPATLPSTIHLDN
                     GQLQCTSCHDPHTDQRPKFLRMDNRYGALCTACHRPAHWNGSTHSTSSATWNGTGTNP
                     WPAGAYPTVAENACLSCHRPHTAGHPQRLLAQNVEPTVCTVCHNGAVASKNIEAEFQT
                     KPYYHPIQSAQWTHDPKENPLIMQRHVACMDCHNPHAVTATPAAPPLVPGRLQGVPGL
                     TLGGSPVAESSFVYEVCFKCHGVSEPTTQGIARWDSTRNIRLKIDPANPSYHPIAATG
                     KNTTMDGFEAGYNTSTIITCTSCHNNDDWTPTGTSPVGPHGSRYEPILERRYESNDPT
                     TESSAVYAMCYKCHNRDYEINDQANTFKHQRHVVRVGAPCAACHDPHGSRQYPHLINF
                     MRFDRTGKNVVTASSGGRLEYIQTGPGKGTCYLTCHGNNHNPRSY"
     gene            complement(5671..6438)
                     /locus_tag="AC16CX_11955"
     CDS             complement(5671..6438)
                     /locus_tag="AC16CX_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013262.1"
                     /translation="MKICHTSGLIMAPLMLVLFIVLGVPLSALADLRPLSRSMLANLQ
                     ATNTIGESLAIEDYAGIEAAAQDLQNRARELQTWDASQLGFRPDSRAQFDGYLKLQEE
                     ISAKIIRFARQKDAPAIVAGFGELLEKSCLACHSNFRDRQGMLKASTLFMTSFVGAWR
                     EINRGLLLNDFTLVERAARALVAIAQVMSWDPVIQASFNLPKDAQRNKFREHLAKMIG
                     AAGRIEDGASRGESGTVQKALTEMWSMGCLSCHKEFR"
     gene            complement(6752..7078)
                     /locus_tag="AC16CX_11960"
     CDS             complement(6752..7078)
                     /locus_tag="AC16CX_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013263.1"
                     /translation="MKPIIAAFFAVASLSLASAAQAADPKAAEELAKKNACMACHAMD
                     KKLVGPAYKDIAAKYRGQKDAEAKLIEKVKKGGQSVWGQVPMPPNAQVKDEDVKTLVE
                     WVLSLQ"
     gene            complement(7214..7987)
                     /locus_tag="AC16CX_11965"
     CDS             complement(7214..7987)
                     /locus_tag="AC16CX_11965"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR3013264.1"
                     /translation="MKLIKLALLVLAMFAVTLATIGVMPSNSWGAPGDGLAGTAHDFS
                     GKAASGGFTTGSCTFCHTPHKAISTLLLWNHTLSTNTFSWDVPTTTAGTNFSTFKGDT
                     YKGPTAKCLSCHDGSVAIGDIAWFGEATRTGVDGLDAMKHGAGDTFNVGFGGSMAGNH
                     PVAMPYPYLQGANTYNGVTTGGAATLTEWQATPLSPVRVFNDDGSGNITGGAVATKTG
                     IECSSCHDPHNKAAVDDLFLRGTIGGNTTAYLCLKCHIK"
     gene            8411..8656
                     /locus_tag="AC16CX_11970"
     CDS             8411..8656
                     /locus_tag="AC16CX_11970"
                     /inference="COORDINATES: protein motif:HMM:NF048679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI/CycH"
                     /protein_id="HIR3013265.1"
                     /translation="MPRLAVLRKRVRDLPAAFTLDDSMAMAAGMKLSDHPRVIVGARV
                     SKSGSPTPQAGDLEGLISTPVTAGADRLNVVIDTVVR"
     gene            complement(8803..>9440)
                     /locus_tag="AC16CX_11975"
     CDS             complement(8803..>9440)
                     /locus_tag="AC16CX_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR3013266.1"
                     /translation="GIFLAQLVYFAIVLAPKWRTFKWFGHTSFVPSWQDFKDIYAMFK
                     WFLGKGPRPVFGRWSYWERFDYWAPFWGLTIVGGSGLMLWFKELTAAVWPGWIFNVAM
                     LAHGEEAFLAIVFLFTVHFFNNHFRPDKLPPPDISMFTGVVPLEEFAREHILEYEELK
                     KTGQLEKYLVDAPSPQMTLGSKILGIVLLAFGLALLALVLIGFVGTLAAGK"
BASE COUNT         1869 a         2997 c         2740 g         1834 t
ORIGIN      
        1 ttcttcgagg gcgtgccgcc gcccggtaaa gaagtgccgc cggaaccggt cgtgcgccag
       61 ccgcgccgcc cgccgttcac cccccctgcg aagccggtgc cgccgccggc cgtgccggaa
      121 aagcctccta cacccgactg gaccgccctg tttcagaagc tcccgaaggt cgccgacggc
      181 agcgtggaat gggtgcgggc gctcaacgaa aaactgatcg cgccgagacc cgggattgcc
      241 cctgaagcca aggacgaaca acctgtgatc gaccttaacg tggaactcat tcccaggggc
      301 cagccggctt tcaaggtgat atttccccac aaggcccaca cgcaatggct cgcatgcgct
      361 aactgccatc cggcgatttt ccagatgcag cgtggcgccg acccgatcac gatggcgaag
      421 gtcttcgccg gagaatattg cggacgttgt cacggcaagg tggcgtttgc gctcaccggc
      481 tgcgcgcgct gccatgccgc catgcccaaa tgatgcgcag ttcgcttgcc cttgccgtcg
      541 ctctgcttct ggccctcctg cccgcgcgct acggggaggc cgaagtgggc gatattccat
      601 tcacccgcaa ggcagccggg acggaggatt tcccaccggc agtgttcccc cactggctgc
      661 accggatgca gttcaagtgt cacgtgtgcc acgagaacgt gtttcagatg aaggcgggga
      721 ctaatccggt cacgatggac gcgatttacc aaggcaagta ttgcggggcc tgccataacg
      781 gcaagacagc gtttgcaccg atgttcgagc attgctcgcg ctgccatcgc caatgagaca
      841 aggggcggaa gttaccctcg cagtgggaat gatgatcgtc tttatactgc ttcgtccggc
      901 cgagatccct gcccaggagc tgtggggtct gcgcctgggc acctggggag gcgaagtgca
      961 actgggctac ggaagcgacc gccagcaagc gcgcaccgaa ggtggtgtga cgagcgactt
     1021 cgcgcaccag cgctatcggg agcaattgac tatccgcaat caaggctcgt acttcgttga
     1081 cccgcggctc gtcaccggca acctggcgct gaccttcggt ctgtttcagg accaggaacg
     1141 cagcgatggc tcgctgaatt cacgccaggg caaactcatc ggttacgcga ccgatttcac
     1201 cttccttgcg gagaaacctt acagcggcac gctttacgcg aaccgcactc aaaacttcgc
     1261 gacccagccg ttcggtggac acacggatct gacctatgag aatcacggcg ccgtgttacg
     1321 gctgcgcgaa gacagtatcc tcagggactg gggagtcccc tatttcagct ctaacctgcg
     1381 cgtgtatcag gaaaatatca cggagtcgac gacgagcctc ggccagacgt tccagcgcaa
     1441 cgaactccgc aacgtcctta gcgtcgatgg ccacaaaggc ttcgagacct cggacctgga
     1501 cctgcgctac gagtttaccg accacaatga cgccgccttc ccgctgggcg cttttcagac
     1561 ccagatggcg agcctcagct acagcctgga tttcgggccc actttgaatc gccgctggga
     1621 ctcccgcctc aattattatt cccgcaccgg cctcagtcca acgacgtttc tcaccgcgga
     1681 tgagcacgtg cgcatcgatc atttcaagaa cctgtcaacg gattaccgct atctgctcac
     1741 gcgtatcgat acacaggccg gcactaccac gacccataat ggaatctttc acctgcaaca
     1801 ccggctttat cgaaacctca ctaccaatgc gctgctgtcg ggcctgcgcc aggaactgcc
     1861 tgatggcgtc gtcaccggct atgcgggtca gctcgacttc aattatcgac gcggcctgcc
     1921 gcgggacggg cggctgtttg cgtccgccgg cgggcgctat caactgaatg acaacgacct
     1981 cggggcttcg cagatcaacg tcatcgacgc cgcgttcagc gcgccctcgc cgcttggtgg
     2041 cggggccgga tttcttctcg aacaaccttt cgtaattgct tccacaatcg tggtggtgga
     2101 cacacggggc ggcgcgcggc tcccgaccac gctcggcatg gactacgaca tcgtcccgga
     2161 aggcaatctg atcagaattg ttccactgcc ggcgagcctg gtgatccagc cgggcgatcc
     2221 attggcggtg agttacagtt accgggttga tcgcagtctc aaatacacga cggcctcctg
     2281 gtggctgaga agcggtgtgg acttccgctg gattgccttc tccgcagggc acgagcagac
     2341 tgaccaaacg gcgctctcag gcggctccag ccagttcctg gagaaccgac gcaacgacac
     2401 ggcgcaactg gacttgcggg gggcctggaa gatggccgaa gggcaagtag gggccgccta
     2461 ccagcggtat aacgccacct ttctcgccta cactaggcag cggttcaatc agcacgcctc
     2521 gtaccggccg ggaaataact tcgcactggc gttcaacgcg gaagagtcgc atacggattt
     2581 cacgcttcct ttgcgtcaaa gcgatacgcg ctccgcgcag ttgacgacga cctggcttgc
     2641 gcccggcggt tggtggacga cggcccttct cggccggcgt gtttacaaag acagcctgtc
     2701 gcccacggag accgtgaacg aggcgaaggc ccaggcgcgc aagacttacg gcaagttaat
     2761 gatcaattcg gccttcacac gcggtgaccg ggtgcggggc gcgttcgaaa ccgacttctg
     2821 gcgtgtggac gtgaccatca cacggcgatt ctaaggtgga tgatgcgcaa ctttccgctg
     2881 ttgctgatga ccctggtcac agcgctgtcc atgagtggtt gcgagacccc gccctccgcg
     2941 agcgtgacga aggaagttcg cgccggattg ccggtctggc caacacctcc tgatgaaccc
     3001 cgcatccgct acgtgcgcag cgtgtccggg ccggaggatt tcggcatcgc gcgatccttt
     3061 ttccagcgcc tcatcgatgt tttttccggc gcaggcgcgg ttcatttcat tcgccctacg
     3121 ggcgtggccg agcatgatgc ggcgctctac gtcgcagatc cgggtgcccg agcgctgtgg
     3181 atcctcgatc ccgtgaagaa ccggttcgtc aaagtggaga aagtgggcga attagcgctg
     3241 gtctcgccgg tggcggtcgc cgtgcgcgcc gacggcgcgg tttttctcgc cgacaccggg
     3301 ctgaaaaaag tctttcttct ggaccgtgaa ggcaggttca tccgcattgc cgcggaacaa
     3361 ggtctttcac ggccggcggg attggcctat gacgcggcgg gcgagcgact ttacgttgcc
     3421 gactcgatcc atcattggat cggcatttac gacctgaacg gtgccctgat ccggacgtgg
     3481 ggcgaacgcg gcacccggaa tggcgagttc aattccccga cccacctggc gctggaccaa
     3541 acgggaacgc tgctcgtgac ggatgcgctc aattttcgga tccaggcatt tgaccgtgag
     3601 ggccggtttc agtggaagct gggccgccat ggtgacggct cgggcgattt cgcggcgccc
     3661 aagggattgg cggccgacag cgaggggcat gtctacgtcg tggatgcgct gttcgatgcg
     3721 gtgcagatct ttgagcgcga cgggaccctg ctgctggcgt tcggtggaca gggaatccag
     3781 gcaggccaat tctggctccc ggggggcatc ttcatcaccg cccaggacaa aatctatgtg
     3841 gccgatgcct acaaccagcg cgtacaggtg tttgtcttcg gtctcaacgc gggtaaagag
     3901 actaaccgat gacgccggca tcgatgagta atccacgagt gctgcgaacg gtttggcccc
     3961 tgtgcacaac aatcgtgtac agtctcggtg tagtcatgcc gttgctggtg gcgcttgctg
     4021 gttttctggc gctgtccacg ccgacccggg cccagaccgg gatctcgaac accaaacaca
     4081 atctcacgac gaaagtgtcc cagccgacgg gcgtctgcgt cttctgccat accccgcaca
     4141 atgccaaccc gacgcgcgcg ttgtggaacc gggcactgcc gggcgtcacc taccagctct
     4201 acgccagcag cacgctcaaa gcgcaacttg atcagccgac cggcacctcg cgcctgtgcc
     4261 tgtcctgcca cgacggtata ctggctttgg gcgatctgcg cgtgccgcct cccggtgggc
     4321 cgcttgcgct cggaccgctc accggtgaga ccgtcctcgg caccgacctc tcggacgatc
     4381 acccgatctc ttttacctat gacagcacgc tcgcgctgaa ccgcggcggg ctggccgatc
     4441 ccgccacctt gccttccacc atccatttgg acaacggcca gcttcagtgc acgagctgtc
     4501 acgatcccca taccgaccag cgaccgaagt tcctgcgcat ggacaaccgt tacggggcgt
     4561 tatgtaccgc gtgccatcgc ccggcccatt ggaatggttc gacccactcc acctcgagcg
     4621 ccacctggaa tgggaccgga accaatccgt ggccggctgg cgcttacccc actgtcgcgg
     4681 agaacgcctg cctgagctgc caccgtccgc acactgccgg ccatccgcaa cggctgctgg
     4741 cccaaaacgt ggagccgaca gtctgcaccg tctgccacaa cggtgcagtc gcatccaaga
     4801 acatcgaggc ggagttccag acgaagccgt actaccatcc aatccagagc gctcagtgga
     4861 cccacgaccc caaggaaaat cccctgatca tgcagcggca cgtcgcctgc atggattgcc
     4921 ataatcccca cgccgtgacg gccacgccgg ctgccccacc gcttgttccc ggacggctgc
     4981 aaggcgtccc cggcctcacg ctcggcggct caccggttgc cgagtcgagt ttcgtgtacg
     5041 aggtctgctt caaatgccat ggagtaagcg aaccgaccac gcagggcatc gcgcgctggg
     5101 acagcacgcg caacatccgg ctcaagatcg acccggccaa tccgtcgtat catcccatcg
     5161 cggccaccgg caagaacacc acgatggacg gtttcgaggc ggggtataac acctcgacga
     5221 tcatcacttg cacgagctgc cacaacaacg acgactggac gcccacggga acgagtccgg
     5281 tagggccgca cggctcgcgc tacgagccga ttctggaacg ccgttacgag tccaacgacc
     5341 ccacgaccga gtcaagcgcg gtttacgcga tgtgctacaa gtgccacaac cgcgattacg
     5401 aaatcaatga tcaggccaac accttcaagc accagaggca cgtcgtccgc gtaggcgcgc
     5461 cttgcgccgc gtgccatgat ccccacggct cgcgccagta tccgcatctg atcaatttca
     5521 tgcgcttcga ccgcaccgga aagaacgtgg tcactgcttc aagcggcggc cgtcttgaat
     5581 acattcaaac ggggccaggc aaaggcactt gttatctcac gtgccacggg aacaatcaca
     5641 acccacgtag ctactaggta cggaaccgga tcagcgaaac tccttatgac aggaaaggca
     5701 gcccatgctc cacatttcag tcagcgcttt ctggactgtc cccgactctc cgcgactcgc
     5761 gccatcctcg atgcggccgg cggctccaat catcttggcc agatgctcac ggaacttgtt
     5821 gcgctgcgcg tctttcggga ggttgaacga ggcctgtatc accggatccc atgacatcac
     5881 ttgagcaatc gctaccagcg ctcgcgcagc cctttccaca agcgtgaagt cgttcagcag
     5941 cagacctcgg ttgatctctc tccaggcgcc gacgaagctg gtcatgaaca gggtcgaagc
     6001 tttcaacatc ccttggcgat ccctgaaatt gctgtggcat gccaggcagg atttttcgag
     6061 cagctcgcca aagcccgcaa cgatcgcagg ggcgtctttc tggcgggcga atcttatgat
     6121 cttggcggaa atttcttcct gtagcttcag atagccatca aattgcgctc tactgtcggg
     6181 tctgaaaccg agctgagagg catcccacgt ctgcagttcg cgtgcgcggt tttgaaggtc
     6241 ctgagcggcg gcctctatgc cggcgtaatc ctctatggca agggactccc ctatggtatt
     6301 ggtcgcttgc aggttcgcga gcattgatcg tgacagcggt ctcaggtcgg cgagcgcgct
     6361 gagaggcacc cccaacacaa taaacaggac caacatcagc ggggccatta ttaaaccgct
     6421 ggtatggcag attttcacct gcggccctcc ttcgttgaac atatgggcac cggccttacg
     6481 gctttgcagt ccttagacgt ttcttcagac tgaataccag cttgcgctga atcgacgagc
     6541 aaataataaa cgaagatatt tgcggaacat cataccggaa cctgccccga aaatccgtcg
     6601 gtcgcgttcg cacctcgatt cgttaagcga acagcaggga tgtccccgga tcaaatccgg
     6661 ggaatactga ccgtatatgg gcctgcagat atggtggctt gagaggatgg cgggggtgac
     6721 cccgcaagct gctcgattga acgcttgcag gttactggag cgaaagcacc cattccacca
     6781 gcgtcttgac gtcctcgtcc ttaacctgcg cgttgggcgg catcggcacc tggccccaga
     6841 cgctctgccc gcctttcttg accttctcga tcagtttggc ttcggcgtct ttctgcccgc
     6901 ggtacttggc ggcgatgtcc ttgtacgcgg gaccgaccag cttcttgtcc atggcgtggc
     6961 acgccatgca cgcattcttc ttggcgagtt cttcggccgc cttcggatcg gccgcttgcg
     7021 cggcgcttgc cagcgacaga ctggccacgg cgaagaaggc cgctatgatt ggcttcatgc
     7081 ttgctccttt acaaggaaaa aaactgtgtt taaccggcac ggccggcccg gattttcccg
     7141 cgcccacggg cccgcttgac gcaaaaaaat gggggctgac agcgcatcag cccccatatt
     7201 tcttcctaca ttcttacttg atgtggcact tcaggcagag gtaggccgtg gtgttgcccc
     7261 cgattgtgcc gcgcaggaaa agatcgtcca ctgcagcctt attgtggggg tcgtgacagg
     7321 atgaacactc aataccggtc ttggtggcca cagccccgcc ggtgatattt ccactcccgt
     7381 catcattgaa cacacggacg gggcttagtg gagtcgcctg ccactccgtc aacgtggctg
     7441 caccgccggt ggtgacgcca ttgtaggtgt tcgcgccttg caggtaagga tacggcatgg
     7501 ccaccggatg gttacccgcc atgctgccgc cgaagccaac gttaaacgtg tcccctgccc
     7561 cgtgcttcat tgcgtcaagg ccgtcaacgc ctgtgcgcgt ggcttcgccg aaccacgcaa
     7621 tgtcaccgat tgcgacgctg ccgtcatgac agctcaggca ctttgctgtg ggtcccttat
     7681 acgtgtcacc tttgaaggtc gagaagtttg tgcccgccgt ggtggtgggg acgtcccacg
     7741 agaaagtgtt ggtcgaaagc gtgtggttcc agagtaagag ggtactgatc gccttgtgcg
     7801 gcgtatggca gaacgtgcac gaccccgtgg taaacccccc cgaggcggcc ttccccgaga
     7861 aatcgtgtgc tgttccggca agcccgtcgc ccggcgcacc ccacgaattc gagggcatta
     7921 ccccgattgt cgcaagcgtc accgcgaaca tcgcgagcac cagaagtgct aatttgatca
     7981 atttcatgac gtgtctcccg tttgggttat cgcattcccg catttcgcat cactacacgc
     8041 acaatgcact ccgggcagca ctactgccct caccgggatg accagcatcg tttatgccag
     8101 tcatcaaaaa agtccgttga gaaaacgacc ggcgtgcgcc ctccttgcaa gttactgtgc
     8161 ggtaaggatt tttcccggaa aaggggatga atactgacgg gcttaacgag taattctcat
     8221 tcctcgcgcg atgcgcctga ccttcttttc ccgcgaataa gaaaatgcgg ctaggatttc
     8281 tcactgataa gaagacagaa atgagctcgc gcttattact tttctagatc aagctcagcc
     8341 atacgaagta acgcgcgtga tgagtctccg cgacacacgg aggctgccgc gctttcacaa
     8401 cgaaaaccgg gtgccgaggc tcgctgtcct gcgcaagcgg gttcgcgacc ttcccgccgc
     8461 cttcacgctc gacgactcga tggcgatggc ggcggggatg aaactctccg atcacccgcg
     8521 ggtgatcgtg ggcgcgcggg tctcgaagag cggcagcccc actccgcaag cgggcgatct
     8581 cgagggattg ataagcacgc cggtgacagc cggcgccgac cgcttgaacg tcgtgatcga
     8641 caccgtcgtt cggtagctcc gctctccgga gtcactcggc gccgccgatg ccgctgcaat
     8701 cttgcggctc gcgcgtcact cctggcccga attcattcag cggtggcggg attgcgcgcg
     8761 gcacgcttcc gcctctcgcg ggacgccgat ttcgtcaggc gatcacttgc cggcggccag
     8821 agttccaaca aagccgatca gcaccagggc gagcagcgcc agcccgaagg cgagcagaac
     8881 gatgcccagt atcttcgagc ccaaggtcat ctgcggcgac ggcgcgtcca ccagatactt
     8941 ttcgagctgg ccggttttct tgagctcctc gtactccagg atgtgctcgc gcgcgaattc
     9001 ctccagcggc accacgccgg tgaacatcga gatatccgga ggaggaagct tgtccggcct
     9061 gaagtggttg ttgaagaagt gcacggtgaa caggaacacg atggcaagga acgcctcctc
     9121 cccgtgagcg agcatagcga cgttgaaaat ccatcccggc cagaccgcgg cggtcagctc
     9181 cttgaaccag agcatgagcc cgctgccccc gacgatcgtc aagccccaga acggcgccca
     9241 gtagtcgaac cgttcccagt aggaccaccg gccgaacacc ggtcgcggcc ccttgccgag
     9301 gaaccacttg aacatggcgt agatgtcctt gaaatcctgc cagctcggca cgaacgaggt
     9361 gtgcccgaac cacttgaagg tccgccactt cggcgcgagc acgatggcga agtagacgag
     9421 ctgcgcgagg aagattccca
//
LOCUS       DBNGWZ010000404         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1935821, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000404 DBNGWZ010000000
VERSION     DBNGWZ010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1935821"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC16CX_11980"
     CDS             complement(<1..571)
                     /locus_tag="AC16CX_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013267.1"
                     /translation="MLNSLAQTIIKASGLGKQVTTGDSELTILSDVSFEVRAGEAVAI
                     VGVSGSGKSTLLGLLAGLDTPSLGSVRIDGHDLFALDEDGRAALRGRMVGFVFQSFQL
                     LPAMTALENVMLPLELAGADDARAEAQIMLKRVGLGDRLHHYPKQLSGGEQQRVAIAR
                     AFVTRPKLLFADEPTGNLDATTGAQVIDLL"
     gene            543..1181
                     /locus_tag="AC16CX_11985"
     CDS             543..1181
                     /locus_tag="AC16CX_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR3013268.1"
                     /translation="MMVCARLFSIVVYSVVACALALSAASSFAATILVYGDSLSSGYG
                     LPVEQGWVSLLERRLHAEKFDYKVVNASISGETTFGGVSRIQDALKTHRPVIVILELG
                     ANDGLRGADIDAVRSNLEAIADACRRAGARTLLIGMHLPPNYGTAYAEKFRLVFSETA
                     RRKKLPLVPLLLEGFAGKREYFQADGIHPTAEAQPMMLNTVWKELLPLLKRR"
     gene            complement(1229..1891)
                     /locus_tag="AC16CX_11990"
     CDS             complement(1229..1891)
                     /locus_tag="AC16CX_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011326331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3013269.1"
                     /translation="MSDHNFKSDLFEQFARIGKALSSGKRLEMLEFLAQGERSVEQLA
                     RMTALSIANASQHLQQLRQVGLVTARKEGLYVYYRIAGDGIVRLLTALRAVGEEHVAD
                     VDRLVKLFLNSKDNLEPVPAKELWQRVKQGLVTVLDVRPPEEYAQGHIRGAINVPVTE
                     LKKRLHQIPAGQEVVAYCRGPYCLLAYEAVQLLRQKGIKARRLEEGYPEWKSAGLPVD
                     AP"
     gene            1998..2840
                     /locus_tag="AC16CX_11995"
     CDS             1998..2840
                     /locus_tag="AC16CX_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR3013270.1"
                     /translation="MDAWLLTHATGVRLGVFFGVFALMAACEIMAPRRTLRESKRVRW
                     TSNLGITILNGVLLRILFPAAAVGAAAFVAGRGAGLLNIFAVPYPLALVLSLLALDLA
                     VYLQHLVFHAVPILWRVHRMHHADLDFDVTTGSRFHPIEMILSMLIKLAVIVLLGPPV
                     LAVLVFEVVLNATAMFNHLNVRIPQPWDRMLRWLIVTPDMHRVHHSVDASECNSNFGF
                     NLPWWDRLFGTYRRQPAAGHEGMVIGIRQFRSSRDLWLDQMLLQPFRDQSGGYAIERP
                     QEAR"
     gene            2840..3190
                     /locus_tag="AC16CX_12000"
     CDS             2840..3190
                     /locus_tag="AC16CX_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HIR3013271.1"
                     /translation="MKTLFILNDPPYGSERSYNALRLARSLLKTSDAEVKVFLIGDAA
                     ACAKAGQKVPQGFYNIGDMLGMAQRAGAEVGVCGTCIDARGIAEADLVQSAHRSTMDE
                     LTAWTQWADKVLVF"
BASE COUNT          578 a         1035 c         1001 g          654 t
ORIGIN      
        1 acagcaggtc gatcacctgc gctccggtcg tggcgtccag gttgccggtt ggttcgtctg
       61 cgaacagcag cttgggccgg gtcacgaacg cgcgcgcgat cgccacgcgt tgttgctccc
      121 cgcccgagag ctgcttcggg tagtgatgaa gacgatcgcc gagacccacg cgcttcagca
      181 tgatctgcgc ctccgcgcgc gcgtcgtccg caccggcgag ctcgagtggc agcatcacgt
      241 tctccagcgc ggtcatcgcc ggcagcaact ggaatgactg gaacacgaac ccgaccatgc
      301 gcccccgcag cgcggcgcgt ccgtcctcgt cgagcgcgaa cagatcatgg ccatcgattc
      361 tcacggatcc caggctcggc gtgtcgagcc ccgcgagcag cccgagcaaa gtggacttcc
      421 ccgatcccga aacaccgacg atggcgaccg cctccccggc acggacctcg aaactcacgt
      481 cgctcaagat ggtcagctcg gaatcaccgg tggtaacctg cttgccgagt cccgaggcct
      541 tgatgatggt ctgcgccagg ctgttcagca tcgtcgtgta ttcagtggtc gcatgcgcgc
      601 ttgcgctgtc ggccgcgtcg tctttcgccg cgaccatcct ggtctatggc gacagccttt
      661 cctccggata cggcttgccc gtggagcaag ggtgggtcag cctgctggag cggcgcctgc
      721 atgcggaaaa atttgattat aaggtagtca atgcgagcat cagcggcgaa accacgttcg
      781 gcggcgtgag ccgcattcaa gacgcgctca agacacatcg gcccgtgatc gtcatcctgg
      841 aattgggcgc caacgacggc cttcgcggcg cggacatcga tgcagtgagg tcaaacctag
      901 aagcgatcgc tgatgcctgc cgccgcgccg gtgcgcgtac cctgctcatc ggcatgcacc
      961 tgcctcccaa ttacggcacg gcctacgccg aaaagttccg gcttgtcttc agcgaaaccg
     1021 ccaggagaaa gaagctgccg ctggtgccgt tgctgctcga ggggtttgcc gggaagcggg
     1081 agtatttcca ggccgatggc atccacccga ccgcggaggc gcagccgatg atgctcaaca
     1141 cggtgtggaa ggaattgctg ccgctgctca agcggcggtg aatcgtgaat ggtgagtagt
     1201 gaatggacaa cagcctgtgt tgcgggccct acggcgcgtc caccggcagc cccgcgcttt
     1261 tccattccgg gtaaccctct tccagacggc gcgccttgat ccctttctgg cgcagaagct
     1321 gcactgcctc gtaggccagc aggcagtagg gaccacggca gtaggcgact acttcctggc
     1381 cagccgggat ctgatgcagc ctttttttca gttcagtcac tggcacgttg atcgcaccgc
     1441 ggatgtgccc ctgggcgtat tcctcgggcg gacgcacgtc cagaaccgtc accagtccct
     1501 gcttgacccg ctgccacagc tccttggccg gcacgggttc caggttgtct ttgctgttga
     1561 gaaacagctt gacgagccga tccacgtcgg ccacgtgctc ctctcccacc gcgcgtaggg
     1621 cggtcaacag ccggacgatg ccgtctccgg cgattcgata gtagacgtaa aggccttcct
     1681 tgcgcgccgt caccagcccc acctggcgca actgctgaag gtgttgcgag gcgttggcaa
     1741 tggacagcgc cgtcatgcgc gccagctgct cgacgctgcg ctcgccctgc gccagaaact
     1801 ccagcatttc caggcgcttc ccgctggaca acgccttgcc gatacgcgcg aactgctcga
     1861 acaagtcgct cttgaaattg tgatctgaca tcgcaccacc ctgtgcgccg cttgactatt
     1921 caagtaatca attgaataat actaccacaa tatttgcccc ggtttaaacc gcaacagaaa
     1981 cctccggttc tccatccatg gatgcgtggc tgctcactca tgcaacgggc gtccggctcg
     2041 gcgtgttctt cggggtcttc gccctgatgg cggcgtgcga aatcatggcg ccgcggcgca
     2101 cattgcggga atccaagcgg gtccggtgga caagcaatct gggcatcacc atcctcaacg
     2161 gcgtcctgct gcgaatcctg tttccagcgg ctgcggtggg tgcggccgcg ttcgtcgccg
     2221 ggcgcggcgc ggggctgctc aacatcttcg cggtgcccta cccgcttgcg ctggtgcttt
     2281 cgctgctggc actggacctg gcggtctatc tgcagcacct ggtgttccac gcggttccca
     2341 tcctgtggcg ggtgcatcgc atgcatcatg ccgacctcga cttcgacgtg accaccggca
     2401 gccgctttca tccgatcgag atgatcctgt ccatgctcat caagctcgcc gtgatcgtcc
     2461 tgctcggccc gccggtgctg gcggtcctgg tattcgaagt ggtgctcaat gccacggcga
     2521 tgttcaacca cttgaacgtc cgcattccgc agccgtggga ccggatgttg cgatggctta
     2581 tcgtgacgcc cgacatgcac cgcgttcatc attccgtcga cgcgtcagaa tgcaacagta
     2641 acttcggctt caatcttcca tggtgggatc ggctgtttgg cacctatcgg cggcagccgg
     2701 cagccgggca tgagggaatg gtcatcggga tcaggcaatt ccgctcatcg cgggatctat
     2761 ggctcgacca gatgttgctt cagccatttc gcgatcagag cgggggctac gccatcgagc
     2821 gtccgcagga agcgcggtga tgaaaacatt gtttattctg aacgacccgc catacggcag
     2881 cgaacgcagc tataacgcgt tgcgtctcgc gcggtccctg ctcaagacaa gcgacgccga
     2941 ggtcaaggtg ttcttgatcg gcgatgctgc cgcctgcgcc aaggccggcc agaaagtgcc
     3001 tcagggtttt tacaacattg gcgacatgct gggcatggca cagcgtgccg gtgccgaggt
     3061 tggcgtatgc gggacctgca tcgatgcgcg tggtatagct gaagccgatc tggtgcagag
     3121 cgcccatcgc agcacgatgg acgaattgac cgcgtggacg caatgggccg acaaggtcct
     3181 ggtgttttga ggcacgcatg agtgctgcgg gcaagaccat cctgattttg ggcgctggat
     3241 ttggcggcat cgtcgcggcg tgcaggct
//
LOCUS       DBNGWZ010000405         5145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1936549, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000405 DBNGWZ010000000
VERSION     DBNGWZ010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1936549"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC16CX_12005"
     CDS             <1..393
                     /locus_tag="AC16CX_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3013272.1"
                     /translation="DVAFTRLVKRDPEMRLPVNRLLQEHRVIAMAGEQLLQYLDGIAA
                     DVFVERATVEAAAATYLTYYRHHLAAEEAEVLPRAAQLLTRADWAAVTAAVPAGRDPL
                     FGDDFEARYRELRRRIAREGSKSVNLDS"
     gene            complement(527..1786)
                     /locus_tag="AC16CX_12010"
     CDS             complement(527..1786)
                     /locus_tag="AC16CX_12010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR3013273.1"
                     /translation="MSPFDRNANRRQFLRFLAGSPLLACGGLEALAAEGPKAGTKLPD
                     PIMWAPLRTEDLIKSPKEAINVFDFEPVMRQNVPPAHFGYMASGIDDEVTLRANREGF
                     LKFQLRPRRLVDVSKVDMSTEILGVKYGNPIVIAPTGSNRTYHPDGEIEVAKAAKAGN
                     HLQILSTVATTSVEDATEARGAPIWFQLYATNKWEVARAFVMRAEKAGCLAVAVTVDR
                     NGGRNQETLFRLRRTDTRECAGCHDRSSLAANYKRRPMFDGVDLSGLTNIQSSAMNWE
                     FMKRLRDTTRMKIVIKGILAHEDAVLAADNGIDAIIVSNHGARSEDSGRSTIDALPEI
                     VEAVKGRVPILIDSGFRRGTDIVKALCMGATGVCIGRPYLWGLGAFGQPGVERVLELL
                     RIELRAAMQQVGAPTIKHLVPAMVRRA"
     gene            1884..1960
                     /locus_tag="AC16CX_12015"
     tRNA            1884..1960
                     /locus_tag="AC16CX_12015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1918..1920,aa:Arg,seq:acg)
     gene            complement(1980..3032)
                     /locus_tag="AC16CX_12020"
     CDS             complement(1980..3032)
                     /locus_tag="AC16CX_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004200089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3013274.1"
                     /translation="MSLCKRGETWWIDVTAPNGERIRRTAGTSSKALAQEFHDKLKAE
                     LWRIAKLGEKPRRTWNESVVRWLKEASHKATIGSDKMHLRWLDRFLGGKHLDQISRGT
                     VDHITDARLAEGVSNATVNRTLEVLRAILRKCVNEWEWLDRAPQIRMLKEPTRRIRFL
                     MRAEAQRLLAVLPEHLADMVAFTLATGLRRSNVTGLQWSQVDLVRRLAWIHPDQAKAR
                     KAIAVPLNAEAVLILRRWIGKHQTHVFSFRGKPITQVSTKAWYAALDRAGIENFRWHD
                     LRHTWASWHVQQGTPLYALQELGGWESPEMVRRYAHLAAEHLAPYADRLCALRVVEES
                     GNDTNTAQAEKEKGLA"
     gene            complement(2933..3241)
                     /locus_tag="AC16CX_12025"
     CDS             complement(2933..3241)
                     /locus_tag="AC16CX_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3013275.1"
                     /translation="MRTLSLIEAARFLKMHPEEVRRRAKAGIIPGAKAGRAWVFIEED
                     LAAYLRSLYSRTRQALQVTWGKEVAECHFANAVKPGGSTLPLQTENEYAELLGLAAKP
                     "
     gene            complement(3219..3476)
                     /locus_tag="AC16CX_12030"
     CDS             complement(3219..3476)
                     /locus_tag="AC16CX_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013276.1"
                     /translation="MTFKRSDLDWAMGELLELFSQANRLGIFPHELNPNIFHSAWSVL
                     SAQDFEVAQRCYELLMAQITRPLQHLDQNPKKKRYAYSLAH"
     gene            complement(3466..4464)
                     /locus_tag="AC16CX_12035"
     CDS             complement(3466..4464)
                     /locus_tag="AC16CX_12035"
                     /inference="COORDINATES: protein motif:HMM:NF014537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HIR3013277.1"
                     /translation="MDTPYHNEGRSAAPSAEAQPRAGAPRTVIRGESREDSPTSHKAL
                     TDWLNVSFPFDPKRLDPKFFFDCFSEVTGGVFGGMTDRERGLHGWRSSFAFDRGNVLF
                     ACGGQRDTAFLSLSGEGCAFVTDWGKLSNLLKADLRGHITRWDGAADDFEGKHSVDLA
                     VVLYQLGGFKSGGRQPNPRQFGNWLTPDTLGRTFQVGSRENGKLARIYEKGKQLGEPS
                     NPWVRWEVELHAVDRVIPWDVLENPGYYVAGAYPCFDWISSEASRIRTVKAQDAISYS
                     RLKHVSSVAYGALINVMLQREGSAERVVELLRRDGIPRRLAFSEDYLRLQGGGDDV"
     gene            complement(4524..4889)
                     /locus_tag="AC16CX_12040"
     CDS             complement(4524..4889)
                     /locus_tag="AC16CX_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013278.1"
                     /translation="MVKPRKPVHIPPLSAKEAQDLVKQIEQATVNFRGLFDELETAIG
                     MLMIGRLVGWKVLVLIHNKRTIRKYEEILGGISIREMFPEEGPLAHKSLGLEIAKKLG
                     NFWKAVSGELKIQERRELT"
BASE COUNT         1010 a         1663 c         1420 g         1052 t
ORIGIN      
        1 gatgtggcgt ttacccgcct cgtcaagcgg gatcccgaga tgcgattgcc ggtaaaccgg
       61 ctgttgcagg agcatcgcgt gattgccatg gccggggagc aacttctcca gtacctcgat
      121 ggcatcgccg cggatgtttt cgtcgagcgg gcaacggtgg aagccgcggc cgcgacttac
      181 ctcacgtatt accgtcacca cctcgccgca gaagaggcgg aagtcctccc ccgtgcggcg
      241 caactactga cacgagctga ctgggcggcc gtaacggccg ccgttccggc aggcagggat
      301 ccgctgttcg gagacgattt cgaagcgcgc taccgggaat tacgccggcg gatcgcgcgc
      361 gaaggatcaa aaagcgtcaa tctcgattca tgacttgccg ccttctgact tctgactttt
      421 gccctagacc caggcgctcc gccctcatcc ccaggcaaaa gacccgcatg ttgcaggcct
      481 tcttgcgcgt ctcgcgcatc tggccatgat gggaaataca accgtttcaa gccctgcgca
      541 ccatggccgg aacgaggtgc ttgatggtcg gggcgccgac ctgttgcatc gcggcacgca
      601 gctcgatgcg cagcagctcg agtacgcgct cgacgcccgg ctggccgaag gcgccgagtc
      661 cccagagata cggccggccg atgcacacgc cggtcgctcc catgcagagc gccttgacga
      721 tgtcggtgcc gcggcgaaag ccgctgtcta tgagaatcgg cacgcggcct ttcacggcct
      781 cgacgatctc cggtagcgcg tcgatggtcg agcgcccgga atcctcgctg cgcgcgccgt
      841 gattggagac gatgatcgcg tcgatcccgt tgtcggcggc gagcacggcg tcctcgtgag
      901 ccaggatccc cttgatgacg atcttcatcc gcgtcgtgtc gcgcagccgc ttcatgaatt
      961 cccagttcat ggcggaggac tggatgttgg tgaggcccga gaggtcgacg ccgtcgaaca
     1021 tcggccggcg cttgtagttg gccgccaggc tgcttcggtc gtggcagccc gcgcattcac
     1081 gcgtgtcggt gcgccgcagc cggaacaagg tttcctggtt gcgcccgcca ttgcggtcca
     1141 ccgttaccgc gacggcaagg caccctgcct tttccgcccg catcacgaag gccctcgcga
     1201 cctcccattt gttggtggca tagagctgga accagatcgg cgcgccacgc gcctcggtcg
     1261 catcctcgac cgaagtggtc gcgaccgtcg agaggatctg aaggtggttg ccggccttcg
     1321 cggccttggc cacctcaatt tctccgtcgg gatgataggt cctgttgctc ccggtcggcg
     1381 cgatgacgat cgggtttccg tatttcacgc cgaggatctc ggtgctcatg tcgaccttgc
     1441 tgacatcgac cagccggcgc ggccgcaatt ggaacttgag gaagccctcg cggttcgccc
     1501 gcagcgtcac ctcgtcgtct atcccggagg ccatgtagcc gaaatgcgcc ggcggcacgt
     1561 tctggcgcat caccggctcg aaatcgaata cgttgatcgc ttccttgggc gatttgatca
     1621 gatcttctgt tctgagcggc gcccacatga tggggtcagg aagtttcgta cccgccttgg
     1681 gaccctcagc cgcgagggct tcgaggccgc cacaggcgag cagcggactt ccagcgagga
     1741 accgcagaaa ttgacgacga ttggcgttcc ggtcgaatgg cgacatgatg ggtttcctcc
     1801 cttggatgaa cgacgacgcg ccgtatgcag gagcgctgac tctgatccct tgtcgcattt
     1861 tgctataatc tcgcctccaa tacgcgcccg tagctcagtt ggatagagta cctggctacg
     1921 aaccaggggg tcgtgggttc aaatcctgcc gggcgcgcca gacacatcaa gggcttgcat
     1981 catgcgagcc ctttttcttt ttcggcttgt gccgtatttg tgtcattgcc cgactcttcc
     2041 actacccgca aagcacatag acgatccgcg tacggagcta gatgctcagc cgcaagatgg
     2101 gcataacgcc gcaccatctc tggactttcc catccaccca actcttgcag ggcatagagc
     2161 ggcgttcctt gctgcacgtg ccagctcgcc caggtatgcc gcaggtcatg ccatcgaaag
     2221 ttctcgatac cggcccgatc cagcgccgca taccaagctt tcgtgctcac ctgggtaatc
     2281 ggcttacccc tgaagctaaa cacatgcgtc tgatgcttcc cgatccagcg ccgcaggatt
     2341 agcaccgctt ccgcattgag cggcaccgcg attgcctttc ttgccttcgc ctgatccgga
     2401 tgtatccacg caagccgccg cactaagtcc acctgagacc attgcagacc agtaacgtta
     2461 ctccggcgca gacccgttgc cagtgtgaac gccaccatat ccgctagatg ctcaggcaac
     2521 acggctaaca accgctgtgc ctcagcacgc atcaggaacc gaatacgccg cgtaggttcc
     2581 ttcagcatcc ggatctgtgg agctcgatcc agccattccc attcgttaac gcattttctg
     2641 agaatggccc gcagcacttc tagcgtacgg ttaaccgtgg cattactaac accctcagca
     2701 agtctcgcat ccgtgatgtg gtcaaccgtc ccgcggctga tctgatccag atgcttaccg
     2761 cccaagaacc gatccagcca gcgtaaatgc atcttgtctg acccaatcgt ggccttgtgc
     2821 gaagcttcct tcagccatct gacgaccgac tcgttccaag tccggcgcgg tttctcaccg
     2881 agcttggcaa tgcgccacaa ctccgctttg agcttgtcgt ggaactcttg cgctagggct
     2941 ttgctgctag tcccagcagt tcggcgtatt cgttctccgt ttggagcggt aacgtcgatc
     3001 caccaggttt caccgcgttt gcaaagtgac actcagcgac ctcctttccc caggtcactt
     3061 gcaacgcttg ccgagtacga gaatagagcg aacgcagata agccgcaagg tcttcctcaa
     3121 tgaaaaccca tgccctcccc gctttcgcac ctggaatgat tccggcctta gccctccggc
     3181 gtacttcctc cggatgcatc ttgaggaacc tagcggcctc aatgagcgag agagtacgca
     3241 tagcgcttct ttttcggatt ctggtctagg tgctgcagtg gccgcgttat ttgcgccatc
     3301 aataactcgt agcagcgttg tgccacttcg aaatcctgcg cgctcagcac cgaccaagcc
     3361 gaatgaaaaa tatttggatt caattcatgc gggaatatgc caagccggtt agcctgcgag
     3421 aaaagctcca gcagctcccc catggcccaa tcaagatcag agcgtttaaa cgtcatcgcc
     3481 cccaccctga agccgcaaat aatcctcgga gaacgcgaga cgacgcggga taccatcgcg
     3541 gcgaagcagc tccacaaccc gctccgcgct tccctcgcgc tgaagcatga cgttgatgag
     3601 tgcgccgtag gcgacggagc taacgtgctt caggcgcgaa tagctgatgg catcctgggc
     3661 ctttacggtg cggatgcggg aggcttcaga ggatatccaa tcgaagcagg gatacgcccc
     3721 cgctacgtag tagccggggt tctccagaac atcccacgga atgacccgat ccaccgcgtg
     3781 cagctcgacc tcccaccgca cccagggatt tgaaggctcg ccaagctgct tgcccttctc
     3841 gtagatgcgg gcgagcttgc cattctcgcg gctccccacc tgaaacgtgc ggcccagcgt
     3901 gtccggggtg agccagttcc caaactgccg gggattgggc tgccgcccgc cgctcttgaa
     3961 gccgccgagc tgatacagca ccaccgccag gtctaccgag tgcttcccct cgaaatcgtc
     4021 tgccgcaccg tcccagcgcg ttatatggcc ccgcagatcg gcttttagga ggttggagag
     4081 ctttccccaa tcggtaacga acgcgcagcc ctcccctgaa agagacagaa aggccgtatc
     4141 ccgctgcccg ccgcaggcaa acaggacgtt tccccgatcg aaggcgaacg acgagcgcca
     4201 cccgtgcaga ccgcgctccc ggtcggtcat gccgccgaaa acgcccccgg tgacctcgct
     4261 gaagcaatca aagaagaact tcggatcgag ccgcttggga tcgaagggaa aggagacgtt
     4321 gagccaatcg gtcagcgcct tgtgggatgt gggggaatcc tcccgacttt ccccccgtat
     4381 taccgtacgg ggggcgccgg cgcgaggctg cgcctctgcg ctcggcgccg ccgaccgtcc
     4441 ttcgttgtga taaggggtat ccatcacccg taatctacgg gtgatagtta ccccttgtca
     4501 agggtatcgg ataccgcttt ccttcaggtg agctcgcggc gctcctgaat cttaagttcg
     4561 ccgcttacag ctttccagaa attgcccagt ttcttggcga tctccagacc cagagatttg
     4621 tgagcgaggg ggccttcctc ggggaacatt tcccggatac taatcccccc taatatttcc
     4681 tcgtacttgc gaattgtccg cttattatga atcagaacca acaccttcca gccaaccaag
     4741 cgtccgatca tgagcatgcc aatagccgtt tccagctcat caaacaggcc tcgaaagttg
     4801 accgtcgctt gctcgatttg cttcaccagg tcttgagctt ccttggcgct tagcggggga
     4861 atatggaccg gcttgcgggg cttgaccata gtagcctcgt gtcagtgaga gaggtaatga
     4921 taaccccgta aggggtaatg agcaaccaga gcagacaatt ccacagaccc cgcaccccag
     4981 tctacagcga aaacagaacc ccacgcctgc gccgccacac actacggcgc ctcgaaacag
     5041 cgcatacctg gacaccaacc acgctgccgc gcaagcttct agaccatcct gagcatcttc
     5101 ttaagctgcc catccccact ttgcccccga ttacatatcc gccca
//
LOCUS       DBNGWZ010000406         1642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1811991, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000406 DBNGWZ010000000
VERSION     DBNGWZ010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1811991"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="AC16CX_12045"
     CDS             <1..593
                     /locus_tag="AC16CX_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3013279.1"
                     /translation="SRREFFSGITRATARAIVPVVVGKGGERGQSGGVRRSKIETAAR
                     SQILAQAAALGEHHGCPLPAELFPALRISDACRNHQVCAATCPTGALQAYANGDGATA
                     GITFDAAACIACGDCTRACPEQALALAPQGDGKVPQGATVLTRWALRECHDCGREFTD
                     FGPGNVCPTCRETRELSRALFGQPLAPAQGQPSERI"
     gene            656..>1642
                     /locus_tag="AC16CX_12050"
     CDS             656..>1642
                     /locus_tag="AC16CX_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3013280.1"
                     /translation="MNPDILNTAISRRRFLQLSGATGAAAAAGAGLEGVTGQPVPEAR
                     AASAESKTVITKNVCNQCPARCGIDVYTTDGRVHAIYGSLESPISNGKLCPKGYLGAY
                     MLYDPDRFTGPMKRTNPRKGRDEDPKFVPISWDEALKTVADRLNALRDKGESHRFALF
                     EGRGWGASDAGLHDAFGKLYGSPNVAIGHSATCADASKKAKLLLEGNYSYNAYDYANT
                     NYMLIFGASFLDAYRPYNNNLQVWGHIRTKSPKTKVTAVDTHLNTTLAAADRALLIKP
                     GTDGALALAIAHVILTEGLWDRNFVGDFKDGVNRFKVGTATLKPEDFNEKWTK"
BASE COUNT          323 a          545 c          525 g          249 t
ORIGIN      
        1 tgagccgccg ggagtttttc tccggaatta cgcgcgccac cgcgcgtgcc atcgtgccgg
       61 tggtggtggg caaaggcggc gagcgcgggc aatcaggggg cgtacgccgc agcaagatcg
      121 aaaccgccgc gcgttcgcag atattggcac aggctgctgc actcggcgag caccacggct
      181 gtccgctccc cgccgagctg tttcccgctc tgcggatctc ggacgcatgc cgcaatcacc
      241 aggtttgcgc cgccacttgt ccaaccggcg cgctgcaagc ctatgcaaac ggcgatggcg
      301 caacagcggg catcacgttt gatgcggcgg cgtgcattgc gtgcggcgat tgcacgcggg
      361 cttgcccgga acaggcgctc gccctggctc cgcaaggcga cggcaaggtc ccgcagggcg
      421 ccaccgtatt gacgcgatgg gcgctgcgtg aatgccatga ctgcgggcgt gagtttacgg
      481 atttcggccc aggcaatgtc tgcccgacct gccgtgagac gcgcgagttg agccgcgcct
      541 tgttcggaca accgcttgct ccggcgcagg gccagccatc ggagaggatt tgatccggag
      601 catcgtttcg cagagaccga aggagaccat cgacttgaac gggaaaggaa cgagcatgaa
      661 ccccgacatt ctcaacaccg cgatctcacg ccgccgcttc ctgcaactga gcggggcaac
      721 tggcgccgcc gcggctgccg gcgcagggct ggagggcgtc accggccagc cggtgcccga
      781 agcaagggcg gcctccgcgg aaagcaagac ggttatcacc aagaacgtct gcaatcaatg
      841 tcctgcgcgc tgcggcattg acgtctacac gaccgacggc cgcgtgcacg cgatctacgg
      901 ctcgctggag agccccatct ccaacggcaa gctgtgcccg aaaggctacc ttggcgccta
      961 catgttgtac gaccccgatc gcttcacggg gccaatgaag cgcaccaatc ccaggaaagg
     1021 ccgcgacgaa gacccgaaat tcgtgccgat atcgtgggac gaagcgctca agacggtggc
     1081 cgaccggctc aacgcgttgc gcgacaaggg cgaatcgcac cggttcgcgc tttttgaagg
     1141 ccgcggctgg ggcgcgtccg acgcgggatt gcacgacgcg ttcggcaagc tctacggctc
     1201 gcccaacgtc gcgatcggcc actccgcgac ctgcgccgac gcgagcaaga aggcgaagtt
     1261 gctgctggaa gggaactact cctacaacgc ctacgactac gccaacacca actacatgct
     1321 gatcttcggc gcgagtttcc tcgatgccta ccgcccgtac aacaacaacc tgcaggtctg
     1381 gggccacatc cgcaccaaga gcccgaagac caaggtcacg gcggtggaca cgcacctgaa
     1441 caccacgctc gctgccgccg accgcgcgct gctgatcaag ccgggaaccg acggcgcgct
     1501 ggcgctcgcg atcgcgcacg tcatcctgac cgagggcttg tgggaccgga acttcgtcgg
     1561 cgacttcaag gacggcgtga accgcttcaa ggtcgggacg gctaccctca agcccgagga
     1621 cttcaacgag aagtggacca ag
//
LOCUS       DBNGWZ010000407        16309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1936926, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000407 DBNGWZ010000000
VERSION     DBNGWZ010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1936926"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(676..2256)
                     /gene="ggt"
                     /locus_tag="AC16CX_12055"
     CDS             complement(676..2256)
                     /gene="ggt"
                     /locus_tag="AC16CX_12055"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3013281.1"
                     /translation="MAVASAHPLATAAGHEILKRGGNAFDAAVAVAAVLAVVEPYASG
                     LGGGGFWLLHRERDGRQVMVDARETAPAGVKRELFLDRSGKPVPGATRQGGTAAAIPG
                     APAALIYVAERHGTLSLAETLAPAIRHARDGFAVDDRYTRVSGLRERFLQNGAGTSRI
                     FLDGNRAPRAGWKLRQPELAGTLERLAKEGRNGFYAGPVAGALVESVNRVGGVWRLSD
                     LAGYRVIERAPLKFRYRNATVTTVALPSAGGIALAQCLNMLERYDLAERRSPETAHLV
                     IEALRRVFHDRARYLGDSDFVAVPVDHLTSKVYAKQRAATINPAVATRSEKLTHEPVA
                     VGGGNTTHLSVVDAEGNRVAATLTINLQFGAGIVAEGTGVLLNNEMDDFTLAPDVPNA
                     FRLRGGDANAIAPGKRPLSSMTPAFVEDEKGVLVLGAPGGSRIVSQVLLAVLDYLRVQ
                     AVDLSAIVGAPRYHHQWWPDRVEIEPAGFAGDWRAALEAKGHRIEVARRGWGNMQAVF
                     KSKQTGAAEAASDPRGAE"
     gene            complement(2371..3021)
                     /locus_tag="AC16CX_12060"
     CDS             complement(2371..3021)
                     /locus_tag="AC16CX_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013282.1"
                     /translation="MIRNLRRALAVSLLLYVLPLKADAAGLYAIDTFVRSDIVTEPTP
                     GNFSVCHAGTCTIVSQVSLDDGQWQRIAAAFPVPARDAQEERARIAEAIARFETVVGP
                     IADTSDDRAGNQRGNQWWSQMDCIDESTNSTTYLRILARMGLLWWHRVEARVTRGYFL
                     FGWPHTTAVVSELGTGAKWAVDSWFHENGRPPEIVPLELWKTGWRPEKPPVSAFPK"
     gene            complement(3104..4237)
                     /locus_tag="AC16CX_12065"
     CDS             complement(3104..4237)
                     /locus_tag="AC16CX_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013283.1"
                     /translation="MNVFSWNRFIAVLVKEFVQMRRDRLTFAMMVGIPIIQLILFGFA
                     INTDPKALPTAVLAADNSIFVRTFVRAMENSGYFRVIRQVAGEHEAERVLAVGEVQFV
                     LTLPENFSRKLVRGERPVLLLEADASDPAATSNALTALINLNQGALARDLTGSLANLQ
                     NGPPPYEVRVHRRYNPEGITQYNIVPGLIGVVLTMTMIMMTALAMTRERERGTMENLL
                     ATPVRPGEVMAGKIIPYIIVGYVQVTIILLAAKLLFEVPIVGSLALLSVVLVLFMAAN
                     LAVGFTFSTLARNQMQAMQMTFFFFLPSMLLSGFMFPFRGMPEWAQWLGEILPLTHFL
                     RVVRGILLKGNEAAEVLPNLWPIAAFLLVAAIIALQRYRETID"
     gene            complement(4234..5163)
                     /locus_tag="AC16CX_12070"
     CDS             complement(4234..5163)
                     /locus_tag="AC16CX_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013284.1"
                     /translation="MSADTAVIDVKGLCKSFGGRRVVDDVSIRVGRGRICGFLGPNGS
                     GKTTTIRMLCGLLKPDSGAGTCLGFDIVRQAGEIKRRAGYMTQRFSLYEDLSIRENLD
                     FIARVYAVPDRGHAVTEALERLGLAQRSDQLAGQLSGGWKQRLALAACLLHDPQLLLL
                     DEPTAGVDPKARREFWDQIHDLAAGGITVLVSTHYMDEAERCHELAYIAYGRLLVTGT
                     GEEVVAHSGLATWTVTGGGLFELAQELRTRPAVAMVVPFGNTLHVSGEDAQALERAVA
                     AYRNRPDLEWRRAEPGLEDAFIHFMREAQDNFQ"
     gene            complement(5250..6227)
                     /locus_tag="AC16CX_12075"
     CDS             complement(5250..6227)
                     /locus_tag="AC16CX_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR3013285.1"
                     /translation="MRSMIRVGAPGIALAVLVVAACSNEVSRSHQGYAEGEYVRVAAP
                     FAGTLQQLAVKRGMQVKAGDTLFTLEQENEAAARREAQERLRNAEAQLANLQKGRRPT
                     EIEAVRAQLAQAESALKLSAVQLKRQEQLVAQNFISKERLDEARASYDRDTQRVVELR
                     AQLATARLAARPDEIKAAEFNVEAAKAALAQAEWRLAQKSVEAPVAGLVQDTYFVAGE
                     WVTANQPVVSLLPPQNIKVRFFAEERHLGAVKIGQKLVISCDGCAAPVAASVSFISPQ
                     AEFTPPVIYSRQERAKLVFLIEARLSPEDAVKLHPGQPVEVRFEASGKP"
     gene            complement(6253..7326)
                     /locus_tag="AC16CX_12080"
     CDS             complement(6253..7326)
                     /locus_tag="AC16CX_12080"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HIR3013286.1"
                     /translation="MKWFDIAVIPGDGIGREVVPAGCRVLDTVAEVHGGFRLRYRSYP
                     WSCAWYLKHGKMMPDDGLKRIGDSDAIFLGAVGFPGVADHVSLWGLLIPIRRGFQQYA
                     CVRPVKLLPGVRSPLAGRGPQDIDFIVVRENNEGEYSSMGGRIYSNTPHEVVTQLNLF
                     TRRGTERIIRYAFGLARRKRKKKVTSATKSNGIIFSMPFWDEVFWQVAKEYPGIAAEQ
                     MHIDALSARFVTHPQNFGVVVGSNLFGDILTDLGAAIAGSIGIGASANLNPEKKFPSC
                     FEPVHGSAPDIVGKGIANPLGQIWSGALMLEHLGIPAASDLVVKAMSSVLAAGIKTPD
                     LGGKASTRQVTQAVAREVRRLAA"
     gene            7482..8456
                     /locus_tag="AC16CX_12085"
     CDS             7482..8456
                     /locus_tag="AC16CX_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013287.1"
                     /translation="MFARIFTQSLAALALLYCVVVPASGQTYPVKPIRIVVGFTPGGG
                     VDINARLLAPELTKQFGQQVIVENRPGAGTNIANELVARAAPDGYTLLINTAAIVINM
                     SLYKKVAYDAMRDFAPISLFSMAPNILVVHASVPVKSVKELVALAKSRPGKLNYSSAG
                     AGTTQHLAGELFNLRAGTKIVHIPYKGSAPSLTALISGEVDLTYANIPAISAHVKAGR
                     LRPLANLGPKRSDQLPGVPTMREAGIKGVEVVVWYGVLAPAATPRDIVTKLADAIGRA
                     ARTSDMKQRLIDLGAEPVGNTPEEFGKLLREELTRWAEVVRVSGARAD"
     gene            8654..10411
                     /locus_tag="AC16CX_12090"
     CDS             8654..10411
                     /locus_tag="AC16CX_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013288.1"
                     /translation="MTPKESASSIAHDAPVRSRESAEYWGSDAFAAILRELDIPYISL
                     NPGASYRGLHDSIVNYLGNRTPQMVLSIHDENAVTIAHGYAKASDRMMAAALHSNVGL
                     MHATMSIFDAWCDRAPVLILGATGPWDAAKRRPWIDWVHTCSDQGALIRDYTKWDNQP
                     GSVEAAMEAMLRGSQIAQTAPRGPVYINLDISLQMGKIGPMPALPDVKRYAAPEPVRP
                     APEQVAAAAKLLSGAKNPLLLAGRCSRSLDAWKARVALAEKLNLRVLTNLKVAAAFPT
                     DHRLHAAPPANRYGPETLKMIADADVILSLDWLDLGGLLKSAYGSKPVTAKIIQVSCD
                     QHSHRGWSMDYQGLPPIDVYLMCEPEAAVPMLLEAAKARTASVPDPVAPKRPAAPADS
                     VSMTGVADALKTATRGIDVCYAHLPIGWSGANSCFRHPLDYLGAEGGAGVGSGPGITV
                     GAALALKGSGRLPIGVLGDGDFLMANTAVWTAAHYQIPCLIVVCNNRSFFNDELHQER
                     VAKERGRPVENRWIGMRIGDPDIDLAALARAQGATGIGPVTRPADLPKAIDQGVEIAS
                     KGGVCVVDARVLPGYDAEG"
     gene            complement(10422..11306)
                     /locus_tag="AC16CX_12095"
     CDS             complement(10422..11306)
                     /locus_tag="AC16CX_12095"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3013289.1"
                     /translation="MAMIPRERVPYSAIVDRPRLVLPDSGRIIVWTIVNLEVWDISKP
                     MARQVIPAPTGAVLLPDVPNWSWHEYGMRVGFWRFHALYERLGMRPTLAVNARVCEDY
                     PRVAQACRDAGWEFMGHSYQQGPIHKEDDQPAMIKRSLDVIEKFTGKRPVGWLGPGLT
                     QTYETPDYLAAAGIRYIGDWVYDEEPTEIRTEHGPLITLPYSVETNDIPMMLVQHHEA
                     DYWTRKCIDAFDRLYLEGMERPRIMAIAIHPYISGQPFRIKYLEAVYDYMARFPSVIH
                     WNGEQILEWYLKARGKAN"
     gene            complement(11326..12282)
                     /locus_tag="AC16CX_12100"
     CDS             complement(11326..12282)
                     /locus_tag="AC16CX_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR3013290.1"
                     /translation="MKICVAGQGAFGQKHVDALKRIPGVEVVSLIGGNPAGTEAVAKK
                     CNISHWGHDLAEGIKRADAVILATPTKMHFRQSEQVMRAGKHVLVEIPVTDSVEDAEN
                     LVKIARETGVTAMGGHVRRFNPSHQWVHKRIEKGELKIQQMDVQTYFFRRKNINAAGN
                     PRSWTDHLLWHHAAHTIDLFAHQCGEAISDCFAVQGPIHPQLNIAMDMSIVAKVPSGA
                     ILTLSLSFNNDGPLGSFFRYICDNGTYKAFYDDLSDGKDNKIDVSKVDVSMDGIELED
                     REFIAAIQQKREPNGSLAQLLPCMRVLGKLEKMIDPDRKAHA"
     gene            complement(12295..13140)
                     /locus_tag="AC16CX_12105"
     CDS             complement(12295..13140)
                     /locus_tag="AC16CX_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HIR3013291.1"
                     /translation="MARIVAGVAASHIPAVGAAWDNGKAGEPYWRPIFQGFERSKEWI
                     AKVKPDVCIVVYNDHASAFSLEIIPTFAIGCAAEFPIADEGWGPRPVPPVKGHPELAS
                     HIVQSVVLDEFDLTIVNKMEVDHGLTVPLTLMFGQPKEWPCPVIPISVNVVVYPVPTG
                     HRCYLLGKAIRKAVESFDRDMRVVIFGTGGMSHQLQGPRAGLINREFDTRFLDNLSKD
                     PQALVKISHFEYLRETGSEGIEMVMWLIMRGALDDEVREVRRFYHVPASNTALGHIIL
                     ENTRR"
     gene            complement(13133..13489)
                     /gene="ligA"
                     /locus_tag="AC16CX_12110"
     CDS             complement(13133..13489)
                     /gene="ligA"
                     /locus_tag="AC16CX_12110"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HIR3013292.1"
                     /translation="MFDADRARLGYHLNMFCMSLMKPENRTAFKADQRAYLDRWPMTE
                     DQKQAVLRRDWNGMLELGGNIYFTSKIAATDGLSFQQIAAIMTGSTQEQYAQMMLNGG
                     RPVDGNRSKSEWKNRG"
     gene            complement(13681..14703)
                     /locus_tag="AC16CX_12115"
     CDS             complement(13681..14703)
                     /locus_tag="AC16CX_12115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3013293.1"
                     /translation="MRILVYGAGAVGGYIGGILTAAGADVTLVARGAHHDAMKQRGLI
                     LEGPKSGRPDPIRVRVCRPGEEKPPYDLIIVGLKSHQIAGAAAHMVGLLARDGMMLLG
                     QNGLPYWYFEKLDSPLRGAQLKSVDPDGTLAKTIPIDAVIGGVMNKPADLVEPGRIRL
                     ADQLTDRLVIGELDNRITPRLAAIKAVIEPAGWPVLISDNIRAAKWRKLLSNAVFNPL
                     GAITQSSARQIADFEPTRRVARRMMDEVVAVAASVGVTPDMTAGQMMEDVQKRVGILS
                     STLQDVRFGRELELDALVNAVIDVGRLTGVPTPCLEMASACAGLLNQRIVDDGVAFAP
                     TALRKA"
     gene            complement(14781..15050)
                     /locus_tag="AC16CX_12120"
     CDS             complement(14781..15050)
                     /locus_tag="AC16CX_12120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR3013294.1"
                     /translation="MEKWLTDGHGHLIAATHGRATLDSAEAAIRGALAGRYDVVAAAL
                     TIGTPVLFSSVGHGSALDIAGQNRASPSALIEAIERLAGARAYNP"
     gene            complement(15087..16046)
                     /locus_tag="AC16CX_12125"
     CDS             complement(15087..16046)
                     /locus_tag="AC16CX_12125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013295.1"
                     /translation="MKPVYILAAILVTLASASAALGQSFPSKTIRLIVPYAAGSVNDT
                     VARIIAPALSDALGRPVVIDNRPGGRGIIGAEVAAKSPPDGYTLMMGNVSHAINVTLY
                     EKLAYDFVKDFAPVSMLATGSYMLVAHPSVPAKSVKELIAFAKARPGQLNFSTSGSGV
                     YLAGKLFESMTGVRMTFITYKSTPQVMTALVSGEGSIAFPGTTAALPHVKSARVRGLA
                     VTSAQRSLVAPDVPTLAEAALPSFEAVPWYGLLVPVGAPKEIISRYHAETVKALNRPD
                     VKERLGATDLVPTGTAPERFATHISSEVAKWGKLVKTYNLHPE"
     gene            complement(16075..>16309)
                     /locus_tag="AC16CX_12130"
     CDS             complement(16075..>16309)
                     /locus_tag="AC16CX_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013296.1"
                     /translation="GFSAIDLRTEMIMRRVEPTQEWLLQESAGMPYGGVIVGMDDADR
                     AAMVREIAAALSQYWKNDSFSIPREIHFVLARK"
BASE COUNT         2917 a         5308 c         5070 g         3014 t
ORIGIN      
        1 gagagaagcg aaacaataat taaactgcca agaccgccaa gaattacgga aggggcatag
       61 ggcgagaatt ctaggattgc tcctcaccga cacccgcagt ctctcccagg ttgattcggc
      121 gttaacgtca acctttccta gattggcctc ccttctaatg cgcgccttcg gagtagtggg
      181 tatgtgaggt tatggagccg ctctccgacc tcacccgata cttccagaaa ctcgcgggaa
      241 tactctcttg acataggcac gtctgaatcc gctcgaagcg tagccacgtg caccaatcgg
      301 cccaatagca tttgggcacg ctcaaatcgg acaatgatcc acgcaacggg ctcgttaata
      361 aataggggcg gctcacaccg ctcctattta tttggcggtt cgattcttgt tcttggttct
      421 ctccgcgtct ctgcggttca attattgtcc tggttctctt ggcatcttgg cggtttaatt
      481 ttgatttatt cttggcggtt cgtatttgtt tgatcggcgt ctatcggcgt tcatcggcgg
      541 ctcgattctt gtttttcttg gcgtcttagc gtcttggcga cttggcggtt caatattgtt
      601 cttggttctc tcggcgtctc ggcggttcaa ttttagtttt gggtttttct ctgtgttctc
      661 tgtggctgat gtttttcact ccgcgccgcg cggatcgctc gccgcctccg ccgcgccggt
      721 ttgcttcgat ttgaacaccg cctgcatgtt gccccatccg cgcctcgcga cctcgatacg
      781 gtgacctttc gcttcgagcg ccgcgcgcca gtcgcccgca aatcccgccg gctcgatctc
      841 cacccggtcc ggccaccact ggtggtgata gcgcggcgcg ccaacgatgg ctgaaaggtc
      901 caccgcctgc acgcgcagat aatccagcac tgccagcagc acctggctga cgatgcgcga
      961 gccgcccggc gcccccagca ccagcacgcc cttctcgtct tccacgaaag ccggcgtcat
     1021 gctggagagc ggtcgtttcc cgggggcgat ggcgttggcg tcgccgccgc gcagccggaa
     1081 cgcgttcggc acgtcgggcg cgagcgtgaa atcgtccatc tcgttgttga gcagcacgcc
     1141 ggtcccctcc gcgacaatgc cggcgccgaa ctggaggttg atggtgagcg tcgccgccac
     1201 gcgattgcct tcggcgtcca ccacggagag atgcgtggta tttccgcctc ctacggcgac
     1261 gggctcatgc gtcaacttct cgctgcgcgt cgcgacggcc gggttgatgg tcgcggcccg
     1321 ctgcttcgca tagactttgc tcgtgagatg atccaccggc acagcgacga aatcgctgtc
     1381 gccgagatac cgggcgcggt cgtggaagac gcggcgcagc gcttcgatca ctaagtgggc
     1441 ggtctcgggc gagcgtcgct ccgcaaggtc gtagcgctcc agcatattga ggcactgggc
     1501 gagcgcaatg ccgcccgcgg aaggcagcgc cacggttgtg acggttgcgt tgcgataccg
     1561 aaacttgagc ggcgcacgct cgatcacgcg gtagccggcg agatcggaaa gccgccacac
     1621 gccgcccacc cggttcactg actcgacgag ggcaccggca accggccccg catagaaccc
     1681 gttgcgtccc tccttcgcca gccgctcgag ggtgccggcg agttcgggct gccgcaactt
     1741 ccagcccgcc cgtggagcgc ggttaccatc gaggaagata cgggaggttc cagccccgtt
     1801 ttgcaggaac cgctcgcgca gaccggagac gcgcgtgtaa cgatcgtcca ccgcaaatcc
     1861 atcgcgggcg tggcggatcg caggcgcgag tgtctcggca agcgataacg tgccgtggcg
     1921 ctccgcgaca tagatcagcg ccgcgggagc gcctggaatt gcggctgccg tgcctccctg
     1981 cctggtggcg ccagggaccg gcttgcccga tctatcgagg aacagctccc gtttcacgcc
     2041 ggccggcgcg gtctcgcgcg catccaccat gacctggcgt ccgtcgcgtt cacggtgcag
     2101 cagccagaac cctcccccgc cgagcccgga agcatagggc tcgaccaccg ccagcactgc
     2161 tgcgacagcg acggctgcat cgaaggcatt gccgccgcgt ttgagaatct catgaccggc
     2221 tgcggtggcg agcgggtgcg cggaggcgac cgccacattt ttttcctgcg cgaccgcgca
     2281 ggaaaaaaat aaaagtacaa aaatcaatga cacgcagccc agcgcgtacc gctgaaccgt
     2341 ctgcataggc attgccatcc ggaaattatt tcatttcgga aaagccgaca ctggaggctt
     2401 ttccggacgc caacccgtct tccacagctc gagcggcacg atttccggcg gccgaccgtt
     2461 ctcgtggaac caggaatcca cggcccattt cgcgccggtc ccgagctcgc tcaccaccgc
     2521 ggtggtgtgc ggccagccga acaggaaata accccgggtg acgcgggctt cgactcgatg
     2581 ccaccagagc aggcccatac gcgcaaggat gcgcaggtag gtcgtcgagt tggtggactc
     2641 gtctatgcaa tccatctggc tccaccattg gttgccgcgc tggttcccgg cgcggtcatc
     2701 cgaggtatcg gcaatcggcc cgacgacggt ttcgaaccgg gcaatggcct cggcgatacg
     2761 ggcgcgttct tcctgagcgt cgcgggcggg aaccgggaac gccgccgcga tccgttgcca
     2821 ttgcccgtca tccaggctca cctggctgac gatggtgcag gtgccggcgt ggcacaccga
     2881 gaaattccct ggcgtcggtt ccgtgacgat gtcgctgcgg acgaaggtgt cgatggcgta
     2941 cagtcctgcg gcgtcggctt tcaacggcag cacatacagc agtaacgaga cggcgagtgc
     3001 tcttcgtagg ttacggatca cgggccacga tcatcaaaac cggtttccaa cacgtcgatt
     3061 ggacaggttc aaggaaccta tgcgcagtca gcgcagaggt tcctcagtcg atcgtctcgc
     3121 ggtagcgctg gagcgcgatt atcgcggcga ccagcaggaa agccgctatc ggccacaggt
     3181 tgggcaggac ttccgcggct tcgttgccct tgagcaggat gccgcgcacc acgcgcagga
     3241 aatgcgtgag cggcaggatt tcgcccagcc attgcgccca ttccggcatg ccgcggaacg
     3301 ggaacatgaa gccggagagc agcatcgaag gcagaaagaa gaaaaacgtc atctgcatcg
     3361 cctgcatctg gttcctcgcc agcgtggaaa acgtaaagcc gaccgccagg ttggcggcca
     3421 tgaacagcac cagcaccaca gacaacagcg ccaagctgcc cacgatcggc acctcgaaca
     3481 ggagcttcgc cgcgagcagg atgatcgtca cctgcacgta gccgacgatg atatagggaa
     3541 tgatcttgcc cgccatcacc tcccccgggc ggaccggcgt cgcaagcagg ttttccatcg
     3601 tgccgcgctc gcgctcgcgc gtcatcgcaa gcgcggtcat catgatcatg gtcatggtca
     3661 gcaccacgcc gatcaacccc ggaacgatgt tgtactgcgt gatgccctcc gggttgtagc
     3721 gccgatgcac tcgcacttcg tacgggggcg gcccgttctg caggttcgcg aggctgccag
     3781 tcagatcacg cgcgagcgcg ccctggttca gattgatcag cgccgtcagc gcattgctgg
     3841 tggcggcggg gtcgctcgcg tcggcctcga gcagcagtac cggccgctcc ccgcgcacga
     3901 gcttgcgcga gaaattctcc ggaagagtca gcacgaactg gacttcgccg acagccagta
     3961 cccgctcggc ctcgtgttcg ccggcaacct gccgtatcac ccggaaataa ccgctgttct
     4021 ccatcgcgcg cacgaaggtg cgcacgaata tgctgttgtc cgccgccagc accgcggtgg
     4081 gcagcgcctt gggatcggta ttgatcgcga aaccgaacag gatcagctgg atgatcggga
     4141 tgccgaccat catcgcgaag gtgaggcggt cgcggcgcat ctggacgaat tccttgacca
     4201 gcaccgcgat gaaccggttc caggagaaca cgttcactgg aagttgtcct gcgcttcgcg
     4261 catgaagtgg atgaaagcgt cttccagccc cggctcggcc cgccgccatt cgagatcggg
     4321 ccggttgcgg taggccgcga cggcgcgctc gagcgcttgc gcgtcctccc cgctcacatg
     4381 cagcgtgttg ccgaacggaa ccaccatcgc gaccgccggc cgtgtcctca gctcctgcgc
     4441 cagctcgaac aacccgccgc ccgttacggt ccaggttgca agccctgaat gcgccacgac
     4501 ttcctccccg gttccggtga cgagcagcct gccgtaggcg atgtaggcga gctcatggca
     4561 acgctcggcc tcgtccatgt aatgcgtgga aacgagcaca gtgatgccgc cggcggccag
     4621 gtcatgaatc tggtcccaga attcgcggcg cgccttggga tcgacccccg cggtcggctc
     4681 gtcgagcaac agcagttgcg gatcgtggag caggcatgcg gcgagcgcga gccgctgctt
     4741 ccagccgccc gagagctggc ccgcgagctg gtccgagcgt tgagcgagtc ctaaccgctc
     4801 gagcgcctcc gtcacggcgt ggccgcggtc tggcacggcg taaacgcgcg cgatgaagtc
     4861 gaggttctcg cggatggaca ggtcctcgta aaggctgaag cgctgcgtca tgtagccggc
     4921 gcggcgcttg atctcccccg cctggcgcac gatgtcgaaa ccgaggcagg tgccggcgcc
     4981 gctgtcgggc ttgagcaggc cgcacagcat gcggatagtg gtggtcttgc cgctgccgtt
     5041 cggaccgaga aaaccgcaaa tgcggccgcg ccccacccgg atcgagacgt cgtccaccac
     5101 ccggcgaccg ccaaagctct tgcagaggcc cttgacgtcg atgaccgcgg tatctgcgct
     5161 cacgctacca tttctcaaaa aaacgtaagc agtgagcggt aagcggtcag ccgtggcgac
     5221 gtgatggcta gattcatacg gcttatcgtt tacggtttac cgcttgcctc gaatctgact
     5281 tccaccggct ggccgggatg gagcttcacc gcgtcctccg gcgagaggcg ggcctcgatc
     5341 aggaacacga gcttcgcccg ctcctgccgg ctgtagatca ccggcggcgt gaattccgcc
     5401 tgcggcgaga tgaagctcac actcgccgcg acgggcgcag cgcagccgtc gcagctgatc
     5461 acgagcttct gtccgatctt caccgcgccc agatgccgct cctcggcgaa aaagcgcacc
     5521 ttgatgttct gcggcggcag cagcgaaacc acgggctggt tggcggtcac ccattcgccc
     5581 gcgacgaaat aggtgtcctg caccaggccg gctaccggcg cctcgaccga tttttgcgcc
     5641 agccgccact ctgcctgggc gagggccgcc ttggcggcct cgacattgaa ctcggccgcc
     5701 ttgatctcgt caggacgcgc cgccagtcgc gcagtggcga gctgagcgcg caattcaacc
     5761 acgcgttgcg tatcgcggtc gtaggaagcg cgggcctcgt cgagccgctc cttcgaaatg
     5821 aaattctggg caaccagttg ctcctggcgc ttgagctgaa ctgcggacag cttgagcgcg
     5881 gattcggcct gcgccagctg cgcgcgcacc gcctcgattt cggtggggcg ccgccctttc
     5941 tgcaggttcg caagctgggc ctcggcgttc ctgagccgtt cctgggcctc gcgccgtgcg
     6001 gccgcctcgt tctcctgctc cagcgtgaac agagtgtctc ccgccttgac ctgcatgccg
     6061 cgcttcacgg caagctgctg cagcgtgccc gcaaacggcg cggcaacccg cacgtactcc
     6121 ccttctgcat agccctggtg actgcgcgag acttcgttcg agcacgccgc caccaccagc
     6181 acggccagcg caatacccgg cgcaccgacg cggatcatcg aacgcatagc agcctgcgga
     6241 cgcctaagcc cgtcaggctg ccaggcggcg gacttctctt gccaccgcct gcgtcacctg
     6301 ccgggtactg gccttgccgc ccagatcggg agtcttgatc ccggccgcga gcaccgagct
     6361 catcgccttc acgaccagat cactcgcggc cggaataccc aggtgctcca gcatcagcgc
     6421 cccgctccag atctggccca gcggattggc gatgcctttg ccgacgatgt cgggcgcgga
     6481 gccgtgcacc ggctcgaagc acgacgggaa tttcttctcg gggttgaggt tggccgaggc
     6541 gccgatgccg atcgagccgg cgatggccgc gccgagatcg gtcaggatgt cgccgaagag
     6601 gttgctgccg accaccacgc cgaagttctg cggatgcgtg acgaaacgcg ccgagagcgc
     6661 gtcgatgtgc atctgctccg ccgcgatgcc gggatattcc ttcgcgacct gccagaacac
     6721 ctcgtcccag aacggcatgc tgaagatgat accgttcgac ttggtcgcgg aggtgacttt
     6781 cttcttgcgc ttcctgcgcg caagtccgaa cgcgtagcgg atgatgcgct cggtgccgcg
     6841 ccgggtgaag agattcagct gcgtcacgac ctcgtgcggc gtgttgctgt agatgcgccc
     6901 gcccatgctc gagtactcgc cctcgttgtt ctcgcgcacg acgatgaagt cgatgtcctg
     6961 tggcccgcga ccagcgagcg gcgagcgcac gccgggcagc agctttaccg gccgcacgca
     7021 ggcgtattgc tggaagccgc gccggatcgg tatcagcaag ccccaaagcg agacgtggtc
     7081 cgccacgccg ggaaaaccga ccgcgccgag aaagatcgca tccgagtcgc cgatgcgctt
     7141 caagccgtcg tcgggcatca tcttgccgtg cttgagatac caggcacaac tccacggata
     7201 cgagcggtag cgaaggcgga agccgccgtg tacctccgcg acggtatcca gtaccctgca
     7261 tccggcgggc accacctcgc ggccgatgcc gtctcccgga atcaccgcga tatcgaacca
     7321 tttcatcaac gcttctccct catgaaatgg ctgttatcat agcacccggc atttttcaaa
     7381 aagcgcggcc cgcaggtagt gactcgttac tggtgttcac ttcacgaacc gccggtcggc
     7441 tttcacagaa cgctcacatc ggacaaaccg ggaagacaat catgttcgca agaatattca
     7501 cgcaatcttt ggccgcgctg gcgctcttgt actgcgtcgt cgtccccgct tcgggccaga
     7561 cgtatccagt caagccaatc cgcatcgtgg tcggattcac gccgggaggc ggcgtggaca
     7621 tcaacgcgcg gctgctggcg cccgagctga cgaaacagtt cggccagcag gttattgtcg
     7681 aaaaccgtcc cggggccggc accaacatcg ccaacgagct ggtagcccgt gcggcacccg
     7741 acggttacac cctgctgata aatacggcgg cgatcgtcat caacatgagc ctatacaaga
     7801 aggtggcgta cgatgccatg cgcgactttg cgccgatctc cctgttctcg atggcgccca
     7861 acatactggt ggtgcatgcc tccgtgccgg tgaagagcgt caaggaattg gttgcgctcg
     7921 cgaagtcgcg cccgggcaag ctcaactact cgtccgccgg cgccggcact acccagcatc
     7981 ttgccggcga actgttcaac ctgcgcgccg gcaccaagat cgtgcatata ccctacaagg
     8041 gcagcgcgcc ttccctcacg gcgctcatca gcggcgaggt tgacctgact tacgccaaca
     8101 tccccgccat ttccgcgcac gtcaaggcag ggcggctccg cccgctcgcc aatctgggac
     8161 cgaagcggtc cgaccagtta ccgggagttc cgaccatgag ggaggccggt atcaagggcg
     8221 ttgaagtggt cgtgtggtac ggcgtgctcg ctcccgccgc tacgccgcgc gacatcgtga
     8281 ccaagctcgc cgacgccatc ggtcgcgcag cgcgcacgtc ggacatgaaa cagcgcctga
     8341 tcgatctcgg cgccgaaccg gtgggcaaca cgccggaaga gttcggcaag ctgctgcgcg
     8401 aggaactgac gcgctgggcc gaagtggtgc gggtgtccgg cgcccgggcg gattaaggga
     8461 tcgacgggag agcgggaggg cgtcagggcg tgagatcgac gcagatctcg ctctcacgat
     8521 ctcccgatct cgcgacctcc cggacttttc cagtcatcgg tggtcgcaac tcattaccca
     8581 tcacccatca ctcatcaccg ttttttaggt taagctacgc cctcccattt tccatcgaca
     8641 tcaggtacag gtaatgacac ccaaagaaag tgcctcgtcc atcgcccacg acgcgccggt
     8701 gcgctcgcga gagagtgccg agtactgggg cagcgacgcg ttcgccgcga tcctgcgcga
     8761 gctcgacatc ccctacatct cgctcaaccc aggggcgagt taccgcgggc tgcacgacag
     8821 catcgtcaac tatctcggca accgcacgcc gcagatggtg ctctcgatcc acgacgaaaa
     8881 cgcggtcacc atcgcgcacg gctacgcgaa ggcatccgac cgcatgatgg cggcggcgct
     8941 gcactccaac gtcggcttga tgcacgccac gatgtcgatc ttcgacgcct ggtgcgaccg
     9001 cgcgccggtg ctgatactgg gcgcgaccgg cccgtgggac gccgcaaaac gccgaccctg
     9061 gatcgactgg gtccacacct gctccgacca gggcgcactg atccgcgact acaccaagtg
     9121 ggacaaccag ccggggtcgg tggaggcggc gatggaagcg atgctgcgcg ggtcccagat
     9181 cgcacaaacc gccccgcgcg ggccggtgta catcaacctc gatatttccc tgcagatggg
     9241 aaagatcggc ccgatgcccg cgctgcccga cgtcaagcgc tacgccgcgc cggaaccggt
     9301 gcggccggcg cccgagcagg tcgcggcggc ggcgaagctg ctgtccggcg cgaagaatcc
     9361 gcttttgctc gcaggccgct gctcgcgcag cctggatgcg tggaaggcgc gcgttgcgct
     9421 cgcggaaaag ctcaatctgc gcgtgctgac caacttgaag gtcgctgccg catttccgac
     9481 agatcaccgc ctgcacgccg cgcctcccgc gaaccgctac gggccggaaa cactgaagat
     9541 gatcgcggat gcggacgtga tcctgtcgct cgactggctc gatctgggcg gcctgctcaa
     9601 aagtgcatac ggctccaagc cggtcacggc aaagatcatc caagtctcgt gcgaccagca
     9661 cagccaccgc ggctggagca tggactacca gggactgccg cccatcgatg tctacctcat
     9721 gtgcgagccg gaggccgcgg tgccgatgct gctggaggcg gcgaaggcgc ggaccgccag
     9781 cgtgccggat cccgtggccc ccaagcggcc ggcggcgcct gcggactcgg tttcaatgac
     9841 cggggtcgct gacgcgctca agaccgcgac ccggggaatc gatgtctgtt acgcccatct
     9901 gccgatcggc tggagcggtg ccaacagctg cttccggcac ccgctcgact atctcggcgc
     9961 cgagggtggc gccggcgtgg gttcaggacc cggcatcacg gtgggcgccg cgctggcgct
    10021 caagggtagc gggcggctgc cgatcggcgt tcttggagac ggggacttcc tcatggccaa
    10081 taccgccgtg tggaccgcgg cgcactacca gattccgtgc ctgatcgttg tctgcaacaa
    10141 ccgctcgttc ttcaacgacg agctgcacca ggagcgcgtg gcgaaagagc gcggccggcc
    10201 ggtcgagaac cgctggatcg gcatgcgcat cggcgacccc gacatcgacc tcgcggcgct
    10261 ggcgcgcgcc cagggcgcga ccggcatcgg cccggtcacg cgcccggccg acctcccgaa
    10321 ggccatcgat cagggcgtcg aaatcgcgag caagggcggc gtatgcgtcg tggatgcgcg
    10381 cgtgctgccg ggctacgacg ccgaagggtg acgaattgcg gtcaattcgc cttcccgcgc
    10441 gccttcaggt accactcgag aatctgctcg ccattccagt ggatgacgct cggaaaacgc
    10501 gccatgtagt cgtacaccgc ttcgagatac ttgatgcgga atggctggcc tgagatgtac
    10561 ggatggatgg cgatcgccat gatccgcggc cgctccatgc cttccaggta gagccggtcg
    10621 aaggcgtcaa tgcacttccg cgtccagtag tccgcttcgt gatgctggac gagcatcatc
    10681 gggatgtcat tggtctcgac cgagtagggc agcgtgatga gcgggccgtg ctcagtccgg
    10741 atttctgtcg gctcctcgtc gtagacccag tcgccgatgt accggatgcc ggccgcggca
    10801 aggtaatcgg gtgtctcgta ggtctgcgtg aggcccggac cgagccagcc caccggacgc
    10861 ttgccggtga acttctctat cacgtcgagt gagcgcttga tcattgcagg ctggtcgtct
    10921 tccttgtgga tgggcccctg ctggtaggag tgccccatga actcccagcc cgcgtccctg
    10981 cacgcttgcg ccacgcgcgg atagtcctcg cacacccggg cgttgacagc cagcgtcggc
    11041 cgcatgccga gccgttcgta gagcgcgtga aagcgccaga aaccgacgcg catgccgtac
    11101 tcgtgccagc tccagttcgg gacatcggga agcagcaccg cgccggtcgg cgcaggaatg
    11161 acctgacgcg ccatcggttt cgagatgtcc cacacctcga ggttcacaat cgtccatacg
    11221 atgatgcgcc cgctgtccgg caagacgagc ctcggtcgat cgacgatggc gctgtaaggg
    11281 acgcgctcgc gcgggatcat ggccatcccg tcacccctct ggtgctcagg cgtgcgcctt
    11341 gcggtcgggg tcgatcattt tctcgagctt gccgagcacg cgcatgcacg ggagcagctg
    11401 ggcgaggctg ccattcggct cccgcttctg ctggatcgca gcgatgaact cgcggtcctc
    11461 cagctcgatg ccatccatgg atacgtccac cttcgacacg tcgatcttgt tgtccttgcc
    11521 gtcgctcaag tcgtcgtaga acgccttgta ggtgccgttg tcgcagatgt agcggaagaa
    11581 cgacccgagc ggcccgtcgt tgttgaatga cagcgacagc gtcaggatcg cacccgacgg
    11641 caccttggcg acgatgctca tgtccatcgc gatattgagc tgcgggtgga tcggcccttg
    11701 caccgcgaag caatcggaaa tggcttcccc gcactggtgc gcgaacagat cgatggtgtg
    11761 ggcggcgtga tgccagagca ggtggtcggt ccagctgcgc gggttgccgg cggcattgat
    11821 gttcttgcgg cggaagaagt aggtctgcac gtccatctgc tggatcttga gctcgccctt
    11881 ctcgatgcgc ttgtgcaccc actgatggct cgggttgaag cgccgcacat ggccgcccat
    11941 ggcggtgacg ccggtctccc tggcgatctt caccaggttc tcggcgtcct cgacgctgtc
    12001 ggtgaccggg atttcgacca gcacgtgctt gccggcgcgc atcacctgtt cgctctgccg
    12061 gaagtgcatc ttggtcggcg tggcgagaat caccgcgtcg gcgcgcttga tgccttcggc
    12121 gagatcatgc ccccaatggg agatgttgca tttttttgct actgcctcgg taccggcggg
    12181 attaccaccg atgagcgaca cgacttcaac gccgggtatg cgcttcagcg catccacatg
    12241 cttctgaccg aaagcgccct gtccggctac gcaaattttc atcgcttctc ccttttagcg
    12301 tcgcgtattt tcgagaatga tatggccgag cgccgtgttt gacgccggca cgtgatagaa
    12361 gcgccgcact tcccttacct cgtcatcgag cgcgccgcgc atgatcagcc acatcaccat
    12421 ttctatgcct tccgagccgg tctcgcgcaa atattcgaag tgggaaattt tcaccagcgc
    12481 ctgggggtcc ttgctcagat tgtcgaggaa tcgcgtgtcg aattcgcgat tgatcaggcc
    12541 ggcgcgcggg ccctgcaatt ggtgcgacat gccgccagtg ccgaaaatca ccacgcgcat
    12601 gtccctgtca aaggactcga ctgccttgcg aatggctttg cccagcaggt agcagcgatg
    12661 cccggtcggc accggataga ccaccacgtt gacggaaatc ggaatgaccg ggcacggcca
    12721 ttccttgggc tggccgaaca tcagggtcag tggcacggtc agtccgtgat ccacttccat
    12781 cttattgacg atggtgaggt cgaactcgtc cagaaccacg gactgcacga tatgcgaggc
    12841 gagctcgggg tggcccttga cgggaggaac cggacgcggc ccccagcctt cgtcggcgat
    12901 ggggaactcg gcggcacagc caatggcaaa cgtcggaatg atctcgagcg agaaagccga
    12961 agcatggtcg ttatagacga cgatgcagac gtccggcttc actttggcga tccattcctt
    13021 ggaacgctcg aaaccctgga atatcggccg ccagtagggc tcaccggcct tgccgttgtc
    13081 ccacgccgcg ccgacggcgg ggatatgcga tgccgccacg cccgcgacga ttctagccac
    13141 ggtttttcca ctccgatttg ctgcggtttc catccaccgg gcggccgcca ttgagcatca
    13201 tctgggcgta ttgctcctgc gtcgacccgg tcatgatggc agcaatctgc tggaacgaca
    13261 gcccatcggt cgccgcgatc ttggaggtaa agtaaatatt gcctccgagc tcgagcatac
    13321 cgttccaatc gcgcctcagg acagcctgct tctgatcctc ggtcatcggc catcggtcga
    13381 gataggcgcg ctgatcggcc ttgaaggcgg tgcggttctc cggcttcatc agcgacatgc
    13441 agaacatgtt gagatggtag cccaggcgcg cccggtcggc gtcgaacacg accgttccgg
    13501 gaagatcgtc atattctttc gggagtggca tcgtccttac gcccaacaaa gccgcagcgg
    13561 atggtcaacc ggcggcgccc tcggcagtgc cgacgcttgc gtgccaatga tagcaaaatc
    13621 aatccgatcg cgacgcgcgc gggattgggg actttcgacc cgcgatccgc gactcgcgac
    13681 tcacgccttc ctgagtgcgg tgggggcgaa tgccacgccg tcgtcaacga tgcgctggtt
    13741 gagcagcccg gcgcaggcgg aggccatttc cagacagggc gtcggcacgc cggtcaggcg
    13801 tccgacgtcg atcacggcgt tgacgagcgc gtcgagctcc agctcgcggc cgaaacgcac
    13861 gtcctgcagc gtcgaggaca ggattcccac ccgcttttgc acgtcttcca tcatctggcc
    13921 ggcggtcatg tccggcgtga ccccgaccga cgccgccacg gcgacgactt cgtccatcat
    13981 ccgtcgtgcg acgcggcggg tcggctcgaa atccgcgatc tgccgcgccg acgattgcgt
    14041 gatggcgccc agcggattga aaacggcgtt tgaaaggagc ttgcgccact ttgccgcccg
    14101 gatgttgtcc gagatcagca ccggccagcc ggcgggctcg atcacggcct tgatcgcggc
    14161 gagccgcgga gtgatgcggt tatcgagctc gccgatcacg aggcggtcgg tgagctggtc
    14221 ggcaagccgt atgcgtccgg gctcgactag gtcggcgggc ttgttcatga cgccgccgat
    14281 cacggcgtca atgggtatgg tcttcgcgag cgtgccgtca gggtcgaccg atttgagttg
    14341 cgccccgcgc agcggcgagt cgagcttctc gaagtaccaa tacggcagcc cgttctgccc
    14401 gaggagcatc atgccgtccc gcgcgagcag gcccaccatg tgcgcggcgg cgccggcgat
    14461 ctggtgcgat ttcaggccga cgatgatgag gtcatagggc ggcttttcct cccccggccg
    14521 gcacacgcgc acgcggatcg gatcggggcg cccgcttttc gggccctcga ggatgagtcc
    14581 gcgttgcttc atggcgtcat ggtgcgcacc gcgcgcaacc agcgtgacgt ctgctccggc
    14641 cgccgtcagg atgccaccga tatagccgcc gaccgcgccg gcgccataga cgagaattcg
    14701 cattcgctga atgtgacgat tactggtgac aattaatggt gacgaaaaac ggttgcgatt
    14761 tgtacgtcac tcgtatctca tcacggatta taggcacggg cgccggccag ccgctcaatg
    14821 gcctcgatga gcgcggaggg cgaggcgcgg ttctggcccg cgatgtcgag cgccgaaccg
    14881 tgccccaccg atgagaacag caccggcgtg ccgatcgtga gtgcggccgc caccacgtca
    14941 taccggccag ccagcgcacc gcgaatggca gcttccgccg aatcgagcgt cgcgcgtccg
    15001 tgcgtggcgg caatcaggtg accatgacca tcagtgagcc atttctccat ccccccaatc
    15061 cgttgggaac acggcggttc caaccctcac tcggggtgca ggttgtatgt cttcaccaat
    15121 ttcccccatt ttgcaacttc gctgctgata tgggtcgcga agcgctccgg ggcggttcct
    15181 gtcggcacga ggtcggtggc accgagccgc tcctttacgt ccggccggtt gagcgccttg
    15241 acagtctcgg catgataccg agaaatgatt tcctttggtg cgccgacagg gaccaataac
    15301 ccataccaag gaaccgcttc gaaactcggc aacgcggcct cggccaacgt cggaacgtcg
    15361 ggagccacaa gcgaacgctg cgcgctggtg accgcaagcc cgcgaaccct cgcggacttg
    15421 acatgcggta atgccgcggt cgtcccggga aacgcgatcg acccctcacc gctcacaagc
    15481 gcagtcatga cctggggagt acttttgtag gttatgaacg tcatcctaac gcccgtcatg
    15541 ctttcgaaga gtttccccgc aaggtaaacg ccgcttcccg aggttgagaa attgagctgc
    15601 cccggccgcg cctttgcaaa cgctattagc tccttcaccg actttgccgg cacggatgga
    15661 tgcgcaacca gcatgtagga accggtcgcg agcatcgaga ccggcgcgaa atccttgacg
    15721 aaatcgtagg cgagcttttc atagagggtc acattgatgg catgagagac gttgcccatc
    15781 atcagggtgt agccatcggg cggagacttg gccgctactt ccgcaccgat aatgccgcgt
    15841 cctccgggcc ggttgtcgat cacgaccggc cgcccgagag catccgaaag agctggcgcg
    15901 atgatgcgtg caactgtgtc gtttacgctt cccgccgcgt acggcacgat gagccgtatc
    15961 gtcttgctcg ggaagctttg cccgagcgcg gcggatgccg aagcgagtgt cacgaggatt
    16021 gccgcgagga tatatacagg tttcatttgc gaatccctct cccaaagagc ctggttactt
    16081 ccgggcaaga acgaagtgga tttcgcgggg aatcgagaag ctgtcgttct tccagtattg
    16141 cgacagtgcg gccgcgattt cccgcaccat ggcggcccgg tccgcgtcat ccattcccac
    16201 gatgacaccg ccgtacggca tgccggcgct ttcctggagc agccattcct gcgtcggctc
    16261 gacgcggcgc atgatcatct cggtacgcag atcgatcgcg ctgaatccg
//
LOCUS       DBNGWZ010000408         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2065637, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000408 DBNGWZ010000000
VERSION     DBNGWZ010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2065637"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          296 a          521 c          550 g          232 t
ORIGIN      
        1 cgtcaatcag caagcgcccc tcctgaaact cgtagaagcg cgcgatgagc ctggcgatgg
       61 aactcttgcc cgcgcccgtg tgcccgacga gcgcaatcgt ctcgcccgcg cggatgccga
      121 gcgagaagtc gcgcaggacg actgccttcg aggtgtagtg gaagccgacg cggtcgaact
      181 cgatctcgcc gcgcagaggc ggaacgggcc gttgggccgc ctgcttcacc gccggttcgg
      241 cgtcaatcag cgcgaacacg cgctcggcgg cggatagccc ggcctgcacc tgcggccaga
      301 acgaggcgag gttgaggaac gggaagtaaa accggtcaac gctctggacg aacaggaacc
      361 acgccgaggc ggcgagcgcg cccgcgatta cggcgtgccc gccccggtag acgagcagcg
      421 ccatcgcgcc gcccgcgatg gcccagagca cggggaacac caccgagagc accaacccgc
      481 gccgcacgtt aatggtgtag gcctgccggt tgacgccgcg gaagtcgtcg tagaccacgc
      541 cctcctgccg gaaattcttg gcgatggcga tgccggagat cgtctcctga atcgtggcgt
      601 tcacgttcgc catggcgcgg ttgccctgcc gggtgacgta ccgcgccagt cggcggaagc
      661 ctatcgcgat gaacagcacg ggcagggtcg agagccacaa cgccagcatg agtccggggt
      721 cggtccgcag caggatgacg gtgagaatga gcaccgccgt caactggctg accgtgtcgc
      781 ccaccagcac agcgacgttg gcaaagtcct gcgtgtcgga cgtgatgcgg ctgacgatgc
      841 ggcccgaact gctctcgtcg aagaacgaca ggtcgtgcgc gacggtggcg cggaaggcgt
      901 ccgagcgcat cgcgagaatc acgtcgccga cgacgcgcgt gagcagccgc cgccggaagt
      961 agttgagcac ccactgcccc acgcctgctc cgcccaccaa ccccacggcc accacgatcg
     1021 tctgcgccga caacgcgccc gacagcgcgc cgatgctccc cgcgatcacc accggcagcg
     1081 acgtgtccag gagcgccgag aggaacagcg tgaccgccac gatggcgatg ccgcgccggt
     1141 acggggcgag gtagcgaagg atgcggcgca cgatctgctc gtcgccgtag cggcggtcgt
     1201 aggcttccgg gtcgaggtag ccgaagaagg acataggcaa agtaggcagc cggcagaaag
     1261 cagtaggcgg ttgactgccc tctgcctact gtcttctgcc ttctgaactc tcgtaccgcg
     1321 cgaatatccg ccggtaagcg tctgagcttg ccatcaattc gtcgtggttc cccatcgcgg
     1381 cgacgcggcc cttgcgcagg acgaggatct ggtcggccca gcgaatctgc gagagacggt
     1441 gcgtgatgag cagggtggtg cggccctcgg cagcgcggaa gatggctttc tgaatctggt
     1501 cttcggtcgc gctgtcaatc gcggaggtcg agtcgtcgag gatcaggatg cgcggattgg
     1561 tgaggaacgc gcgcgcgagc gcgatccgct gccgctgcc
//
LOCUS       DBNGWZ010000409         2095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2947635, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000409 DBNGWZ010000000
VERSION     DBNGWZ010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2947635"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="AC16CX_12145"
     CDS             complement(<1..796)
                     /locus_tag="AC16CX_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR3013297.1"
                     /translation="MQAMEPVYFDHNATTPVDERVLEAMLPYLRGRYGNASSRHELGT
                     RARRAVDVAREQVASLVNVQPSQVIFVSGGTEANNLFIKGAAGYLKPAQVAVSAIEHP
                     CVAKPAQELARQGWKLRKLAVSRNGEIELDDVEHALKEPTGIVSVMLANNETGVIQDV
                     ASVAAHARAAKAWMHTDAVQALGKITVDFAALGAHAMTISAHKIYGPKGAGALVMDKR
                     LQLRPIIHGGGHEQGLRSGTENVPAIVGFGAACQLAAQRMQELAGRQ"
     gene            complement(826..1317)
                     /gene="iscR"
                     /locus_tag="AC16CX_12150"
     CDS             complement(826..1317)
                     /gene="iscR"
                     /locus_tag="AC16CX_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HIR3013298.1"
                     /translation="MRLTTKGRFAVTAMIDLAMHHGSGPVTLAEISGRQKISLSYLEQ
                     LFGKLRRHHLVESVRGPGGGYCLARDMGQVSVSDIILAVDEPIDATQCGGKENCQDDQ
                     KCLTHDLWAALNERIFEFLGGVSLRQLVESQKAKQVTKESGIAQVHDMRETVAKRPTP
                     VSA"
     gene            complement(1409..>2095)
                     /gene="cysE"
                     /locus_tag="AC16CX_12155"
     CDS             complement(1409..>2095)
                     /gene="cysE"
                     /locus_tag="AC16CX_12155"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR3013299.1"
                     /translation="RDPAARNTWEVITCYPGFHAMLFHRMAHRLWNVGLKWLARLVSH
                     LSRWLTGIEIHPGATIGRRFFIDHGMGIVIGETAEIGDDCTLYHGVTLGGTSWNKGKR
                     HPTLGKGVVVGAGAKILGPIMVGDGARIGSNAVVVKEVPPGATAIGIPARIVDARETA
                     QNGRFAAYAVVRDLNDPVAKAIHELIGHCADTDKRIEQILLELKRLGGRIDEQQAHKL
                     DANSLNKMVD"
BASE COUNT          347 a          699 c          630 g          419 t
ORIGIN      
        1 cctgccgtcc tgcaagctcc tgcatccgtt gcgcggcaag ctggcatgcc gcgccgaagc
       61 cgacgatcgc cggcacgttc tcggtgccgg agcgcagccc ctgctcgtgg ccgccgccgt
      121 gaataatagg gcggagctgc aatcgcttgt ccatgacgag cgcgccggcg cccttgggcc
      181 catagatctt gtgcgccgag atcgtcatcg cgtgcgcgcc gagcgcggcg aagtccaccg
      241 ttatcttgcc gagcgcctgc accgcatcgg tgtgcatcca ggctttcgcc gcccgcgcgt
      301 gcgcggccac ggacgccacg tcctggatca cgccggtttc attgttggcg agcatcacgg
      361 aaacgatgcc ggtcggttcc ttgagcgcat gctcgacgtc atcgagctcg atctcgccgt
      421 tgcggcttac ggcaagcttg cgcagcttcc agccctggcg ggcaagctcc tgcgcgggct
      481 tcgccacgca gggatgttcg atcgcgctca ccgcgacctg cgcgggcttc aaatagcccg
      541 ctgccccctt gatgaacagg ttgttcgcct cggtgccgcc ggaaacgaaa atcacctgcg
      601 aaggctggac attgacgagc gaggcgactt gttcccgcgc gacatccacc gccctgcgtg
      661 cccgcgtgcc gagctcgtgc cggctggagg cattgccgta gcggccgcgc aggtacggca
      721 gcatcgcttc cagcaccctt tcgtccacgg gcgtggtcgc gttgtgatcg aaatagaccg
      781 gttccattgc ttgcatctgg atgcggccgg gattgccgaa ccggtctatg ccgagaccgg
      841 cgtcggccgc ttggccaccg tctcgcgcat gtcgtgcact tgggcgatcc cggattcttt
      901 cgtcacctgc ttggctttct ggctttcgac cagctggcgc aggctcaccc cgccgagaaa
      961 ctcgaaaata cgctcgttca gcgcggccca caggtcgtgg gtcaggcatt tctggtcgtc
     1021 ctggcagttt tccttgccgc cgcattgcgt ggcatcgatc ggctcgtcca ccgcgaggat
     1081 gatgtcggag acggacacct gccccatatc ccgggcgaga cagtagccgc cgccgggacc
     1141 gcgcacgctc tccaccaggt gatggcggcg cagcttgcca aacagctgct cgagatagga
     1201 cagcgaaatc ttctgccggc cgctgatctc ggcgagcgtc actggtccgc tgccatggtg
     1261 catcgccagg tctatcatgg ctgtgaccgc aaaacgccct ttcgtcgtca gtctcatggg
     1321 ccacctctca aagtatcgtt aaatcaataa cttatataat acactcaata tattagtatt
     1381 ttcgtcaact atatctatcc cgagtaattt agtcaaccat tttgttaaga gagttcgcat
     1441 cgagtttgtg cgcctgctgc tcgtcgatcc gccctcccag ccgcttcagc tcgagcagga
     1501 tttgctcgat gcgcttgtcg gtgtcggcgc agtgaccgat cagctcgtgg atcgcttttg
     1561 caaccgggtc gttgagatcc ctcacgaccg cgtacgcggc gaagcgcccg ttctgcgccg
     1621 tctcgcgcgc atcaacaatg cgcgccggaa ttccgatcgc cgtcgcgccc ggcggcactt
     1681 ccttgacgac caccgcattg gagcctatgc gcgcgccgtc gccgaccatg atcgggccca
     1741 ggatcttggc acccgccccc accacgacgc ccttgccgag cgtgggatgg cgcttgccct
     1801 tgttccatga ggtgccaccg agcgtgacgc cgtggtagag cgtgcaatcg tccccgatct
     1861 cggcggtttc gccgatcacg atgcccatcc catgatcgat gaagaagcgg cggccgatcg
     1921 tggcgccagg atggatctcg atgccggtga gccagcggga cagatgggaa accagccgcg
     1981 ccagccactt caggccgacg ttccacagac gatgggccat gcggtggaac aacattgcgt
     2041 ggaacccggg atagcacgtg atcacctccc aggtattccg ggccgccggg tcgcg
//
LOCUS       DBNGWZ010000410        12140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3198791, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000410 DBNGWZ010000000
VERSION     DBNGWZ010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3198791"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /gene="ybeY"
                     /locus_tag="AC16CX_12160"
     CDS             complement(<1..292)
                     /gene="ybeY"
                     /locus_tag="AC16CX_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3013300.1"
                     /translation="MNSELGTRSPKLDLTVQYAVRARNLPTRGEFRLWTQAALERDAQ
                     VTVRIVGGGEARALNRHFRGKDHATNVLTFVMRSQPRLEGDLALCAPVIAREA"
     gene            complement(309..1262)
                     /locus_tag="AC16CX_12165"
     CDS             complement(309..1262)
                     /locus_tag="AC16CX_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3013301.1"
                     /translation="MKPVELAFRPVDNQRLANLCGVLDENLRQIETALDVSIARRGER
                     FAVTGRPVRTHLAAQVLQSFYERAKSGLTLEDIQLGLIEAVSPRKPKAADAGPTLITR
                     RPDLHGRTPRQVQYLQNILTHDLTFSIGPAGTGKTYLAVACAVDALEREAEKRIVLVR
                     PAVEAGERLGFLPGDMAAKVDPYLRPLYDALYDLMGFDKVARMFERGAIEIAPLAFMR
                     GRTLNHSFVILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDVTQIDLPKGQKSGLVEAR
                     DVLKKVRGIAFTHFDSEDVVRHPLVQRIVNAYGNYKFRVPS"
     gene            complement(1259..2593)
                     /gene="miaB"
                     /locus_tag="AC16CX_12170"
     CDS             complement(1259..2593)
                     /gene="miaB"
                     /locus_tag="AC16CX_12170"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3013302.1"
                     /translation="MPSRLYIKTFGCQMNEYDSGKMADVLRLAHGMEPTADPAAADVI
                     LFNTCSVREKAQEKVFADLGRVKHYKRANPRLLIGVGGCVASQEGAAIVERAPYVDLV
                     FGPQTLHRLPQMIEARRATGKPQVDVSFPEIEKFDNLPPARVECAAAFVSVMEGCSKY
                     CSFCVVPYTRGEEVSRPFEDVLTEVAELAQQGVREVTLLGQNVNAYRGAMTDGETADL
                     ALLLEYVAAIPGVERIRYTTSHPKEFTQRLIDAHARIPQLAPHVHLPVQAGSDRVLAA
                     MKRGYTVLEYKSIVRRLRARRADISISSDFIVGFPGETEADFEATMKLVEEIGFDASF
                     SFIYSRRPGTPAAELPDLTPHEVKLARLQRLQARIEDQARALSRGMVGTRQRVLVEGA
                     ARKDAAELCGRTANNRVVNFPGPRHFAGRFVDVTITAALPHSLRGEIAAAPH"
     gene            complement(2619..2942)
                     /locus_tag="AC16CX_12175"
     CDS             complement(2619..2942)
                     /locus_tag="AC16CX_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013303.1"
                     /translation="MRRWAWILALAAAWAATSHAQLPRQLPANGKLGELIGGQQPFPL
                     LQINNKILRLSPGGRIIDQHNRTILHGTLPEHAYVLFVEDMNGDVSRIYLLLPEELEQ
                     LQRAR"
     gene            complement(3036..3111)
                     /locus_tag="AC16CX_12180"
     tRNA            complement(3036..3111)
                     /locus_tag="AC16CX_12180"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3076..3078),aa:Met,seq:cat)
     gene            3364..4434
                     /locus_tag="AC16CX_12185"
     CDS             3364..4434
                     /locus_tag="AC16CX_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR3013304.1"
                     /translation="MRTPYKILIMGASYGSLLAAKLLFARHTLKLVCLPAESELINKE
                     GIRVRLPVKGREGLVEIDSRKLPGKLSADGPQAVNPADYDLVALAMQEPQYRSPGVRE
                     LLDGVARARVPCMSIMNMPPLTYLKRIPGLNADACRGAYTDPTVWDGFDPKLMTLCSP
                     DPQAFRPPEEKVNVLQVGLPTNFKAARFDSEAHTAILRQLEADIEAVRFDTGSGKIEL
                     PVKLKVHDSVFVPLAKWPMLIAGNYRCVQKDAMRSIKETVHADIETSRAVYNWVVKLC
                     TSLGADEKDLVPFEKYANAALSLGKPSSAARALFGGAPNIERVDRLVKAIAAQKGMQS
                     RVLDETVALVDARLETNRKKAA"
     gene            4536..5705
                     /locus_tag="AC16CX_12190"
     CDS             4536..5705
                     /locus_tag="AC16CX_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HIR3013305.1"
                     /translation="MRQAFDIVIVGGGLVGLSLARALARSGLSLALVEPQPLAPIPED
                     GSWDSRIYAVSPGCAAFLERCGAWNRLPQERVTRVEAMRVYGDDADARLEFDAYDAGL
                     RELAFIVESRQLQRALWETLFSQDARLYCPANWTALEFQADHAVLRLDDGTELAARLL
                     VGADGSESRVRAEAGITVTPTDYRQLGLVANFFCEKPHRDTAFQWFMRDGVLALLPLP
                     GNRVSMVWSVAQERGRSLLAAPAGELATEVESASRGALGALRLMTPAVAFPLKLQRVR
                     RFVKPRLALVGDAAHNVHPLAGQGVNLGFRDARVLAGVLAERGTERDCGDYALLRRYE
                     RARREDVLATQLTTDGLQKLFNNDAVLLTRARNLGLRLVNWQPLLKNLLVRHAVA"
     gene            5728..6438
                     /locus_tag="AC16CX_12195"
     CDS             5728..6438
                     /locus_tag="AC16CX_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR3013306.1"
                     /translation="MYRRIAICLLGIFGLATGMAGADENRVRQLLQAKFPMMKVESVV
                     RTPYSGLYEAVLDGEIVYTDEGATFLFGGNIYDVRTLPPRNVTQERTEQLAAKILTDA
                     REMAIKRVRGGGARTLFTFEDPNCAYCKALQKELAKVENVTIYTFLMPVLSQDSVEKS
                     VAVWCATDRARAWDELMSNGIVPENDKSCSHPLARISAIARRFQIQGTPAIFLGDGKQ
                     IGGMRSALDIEKALSAVK"
     gene            6425..7183
                     /locus_tag="AC16CX_12200"
     CDS             6425..7183
                     /locus_tag="AC16CX_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003756 - protein disulfide isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HIR3013307.1"
                     /translation="MRSSNRAHAPILIRALRALLPVLAAAWTCSALSAQDIESGIRRA
                     FQAKFPKSAVESVTRTPFTGLYEVVFNGQIAYTDEKLNFVFFGNLYDVRSGVERNLTR
                     ERSAQLAAQTLRKSTDSAIKRVRGNGRRVLYTFEDPDCPYCRELQKELLKLSDVTIYT
                     FLWPILSPDSVEKSKAVWCAKDRAKAWDDLMSRGVAPPGGAKCDTPLEKNAELAQRFG
                     VRGTPAVYLADGNMIGGLVPADKLEQALNTVHSR"
     gene            7763..8119
                     /locus_tag="AC16CX_12205"
     CDS             7763..8119
                     /locus_tag="AC16CX_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3013308.1"
                     /translation="MPQVPDPTYYSARDLDVYPRPIVPLELDRLAGGVPGEAAARFRL
                     VLLIDERGIVSEIAVVEADPPGRLEEALRARLAATGFFPAMKDGRAVKSRVELSVTIG
                     RKGADRQPEKSVSGEW"
     gene            complement(8202..9284)
                     /gene="mltA"
                     /locus_tag="AC16CX_12210"
     CDS             complement(8202..9284)
                     /gene="mltA"
                     /locus_tag="AC16CX_12210"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR3013309.1"
                     /translation="MKPPPAGTAGLLRQVSWSDLPGWQAEDPGEAWDAFLVSCRALAK
                     QDAWRDICVVAESIKSPDREMTRRFFESNLTPFQLSNADGSEEGLITGYYEPLLRGSR
                     KPSARYRHPLYGLPDDLLVVDLTDVYPELKGMRLRGRLEGRRIVPYYDRAQIEQGRGA
                     LTGKEIVWVDDAIELFFLQIQGSGRIALENGETLRVGYADQNGYPYRSIGRFLVESGE
                     LPLEKASMQGIKAWARANPERVTELLNQNASYVFFRELPADTPGPPGALGVPLTPRRS
                     VAVDPGFVPLGAPVYLATTWPLSSRPLNRLMLAQDTGGAIRGAVRADFFWGFGEEAAR
                     EAGRMRQLLRMWVLLPNAFRVPNSQE"
     gene            9592..9726
                     /locus_tag="AC16CX_12215"
     CDS             9592..9726
                     /locus_tag="AC16CX_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013310.1"
                     /translation="MDGFLLLLLEAGVALALLVFIVWWTWPRRRPSDDDDRAGRDGKR
                     "
     gene            complement(9730..10326)
                     /locus_tag="AC16CX_12220"
     CDS             complement(9730..10326)
                     /locus_tag="AC16CX_12220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013311.1"
                     /translation="MSLRISYTLLAPLYDLVARPAFRAARRASLAALPREGHATVLLN
                     GVGTGLDFPFLPRCHRYVGLDLTRAMLRRCARRADGREFVLVQGDSLTLPFRDASFDH
                     AVLHLILAVVPDAVRALSETVRVVRAGGSILVFDKFLKGNQRAPLRRLLSPLAARLVT
                     RLDVVLDDVLPAVPGVRVTSDTPAAARGWFRLLRLKRL"
     gene            complement(10434..10814)
                     /gene="apaG"
                     /locus_tag="AC16CX_12225"
     CDS             complement(10434..10814)
                     /gene="apaG"
                     /locus_tag="AC16CX_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HIR3013312.1"
                     /translation="MTDKKYDIVVEARTAFIPDQSDLRKGRYVFAYTITITNTGTVSA
                     QLVSRHWIITDANNQVQEVRGLGVVGEQPLLRPNESFQYTSGTAIATPVGTMRGSYQM
                     MAEDGVQFDAAIPEFTLSMPRVLH"
     gene            10887..11555
                     /gene="rpe"
                     /locus_tag="AC16CX_12230"
     CDS             10887..11555
                     /gene="rpe"
                     /locus_tag="AC16CX_12230"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3013313.1"
                     /translation="MAKTYRIAPSILSADFARLGEEVQKVVAAGADLVHFDVMDNHYV
                     PNLTVGPLVCEAIRPLTRAIIDVHLMVKPVDRIVPDFAKAGANIISFHPEASEHVDRT
                     IALIKDQGCRAGLVFNPATPLSHLDHVIDKLDLVLVMSVNPGFGGQKFIPQALKKLAA
                     VRTRIEATGRDIWLEVDGGVKVENIAEIARAGADTFVAGSAIFGAKDYKATISTMRAE
                     LAKA"
     gene            11772..>12140
                     /locus_tag="AC16CX_12235"
     CDS             11772..>12140
                     /locus_tag="AC16CX_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3013314.1"
                     /translation="MIDLDGTLADTIPDLAAAANMMLRELGRPELDESLIRNFVGKGI
                     PRLVERALAGGIDGTVPAPMLERALPVYERCYASVNGRRTTMFPGAREGLEALRAAGF
                     PLACVTNKAERFTRPLLDGMG"
BASE COUNT         2154 a         3871 c         3962 g         2153 t
ORIGIN      
        1 gcgcctcgcg agcgatcacc ggcgcgcaca gcgcgagatc gccttcgagc cgcggctggc
       61 tgcgcatcac gaaagtcagc acgttggtcg cgtgatcctt gccgcggaag tgacggttga
      121 gcgcgcgcgc ctcgccaccg ccgacgatcc ggaccgtgac ctgtgcgtcg cgctcgagcg
      181 ccgcctgggt ccagagcctg aattcgccgc gcgtgggcag gttgcgcgct ctcaccgcgt
      241 attgcacggt taaatccagt ttcgggctcc gggttccgag ttccgagttc atgcttccgg
      301 cttgcttttc aacttggaac tcggaacttg taattcccgt aggcgttgac gatgcgctgc
      361 accagcggat ggcgcaccac gtcctcggaa tcgaagtggg tgaaagcaat gccgcgcact
      421 ttcttcagca cgtcgcgggc ctcgacgagc ccgctcttct gccccttcgg caggtcgatc
      481 tgggtgacgt cccccgtcac caccgccttg ctgccgaagc cgatccgcgt gaggaacatc
      541 ttcatctgct ccggcgtggt gttctgcgcc tcgtcgagga tcacgaagga atggttgagc
      601 gtgcgcccgc gcatgaaggc gagcggggcg atttcgatcg cgccgcgctc gaacatcctg
      661 gccaccttgt cgaaccccat caggtcgtag agcgcgtcgt agagcgggcg caggtacgga
      721 tcgaccttgg ccgccatgtc gccggggagg aaaccgagcc gctcgccggc ctccacggcg
      781 gggcgcacca gcacgatgcg cttctccgct tcgcgctcga gcgcgtccac cgcgcacgcc
      841 acggcgagat aagtcttgcc cgtgccggcc gggccgatgc tgaaggtgag gtcgtgggtg
      901 aggatgttct gcaggtactg gacctggcgc ggggtgcgcc cgtgcaggtc cgggcgtcgc
      961 gtgatgagcg tcggaccggc gtccgccgct ttcggcttgc gcgggctcac cgcctcgatc
     1021 agcccgagct ggatgtcctc cagcgtgagg ccgctcttgg cgcgctcgta gaacgattgc
     1081 agcacttgcg cggcgaggtg agtccgcacc ggcctgccgg tcacggcgaa gcgctcgccg
     1141 cggcgcgcga tgctgacgtc gagcgcggtt tcgatctgcc gcaggttctc gtcgagcacg
     1201 ccgcacagat tggcgaggcg ctggttgtcg actggcctga aggcgagctc gacgggcttc
     1261 aatggggtgc ggctgcgatt tcgccgcgca acgagtgcgg caacgcggcg gtgatcgtga
     1321 cgtcgacgaa ccggccggcg aagtgcctcg gccccgggaa gttgaccacg cggttgttgg
     1381 cggtgcggcc gcaaagctcg gcggcatctt tccgggccgc accctccacc agcacgcgct
     1441 ggcgcgtacc gaccatgccg cggctcagcg cccgggcctg atcctctatc ctcgcctgca
     1501 gacgctgcag ccgcgcgagc ttcacctcgt gcggcgtgag gtcgggcaat tctgccgccg
     1561 gcgtgccggg gcgccggctg tagatgaagc tgaaactcgc gtcgaagccg atctcttcga
     1621 cgagtttcat cgtcgcctcg aagtcggcct cggtttcgcc gggaaagccg acgatgaagt
     1681 cggaggagat cgagatgtcg gcgcggcggg cgcgcagccg gcgcacgatg gacttgtact
     1741 ccagcacggt ataaccgcgc ttcatcgcgg ccagcacccg gtccgagccc gcctgcacag
     1801 gcaggtgcac gtggggtgcg agctggggga tgcgggcatg cgcgtcgatc aggcgctgag
     1861 tgaactcctt cggatgggag gtggtgtagc ggatccgctc gacgccgggg atcgccgcca
     1921 cgtactcgag cagcagggcg agatcggcgg tttcgccgtc cgtcatcgcg ccgcggtagg
     1981 cgtttacgtt ctggccgagg agcgtgactt ccctgacgcc ctgttgcgcc agctcggcca
     2041 cctcggtgag aacgtcctcg aacggccgcg atacctcctc gccgcgggta tacggcacca
     2101 cgcagaaact gcagtacttg ctgcagcctt ccatgaccga gacgaaagca gccgcgcatt
     2161 cgacccgggc gggcggcagg ttgtcgaatt tctcgatctc ggggaacgac acgtccacct
     2221 gtggcttgcc ggtcgcgcgg cgcgcctcga tcatctgcgg cagccggtgc agcgtctgcg
     2281 gaccgaacac gaggtccacg tacggggcgc gctccacgat ggccgcccct tcctggctgg
     2341 cgacgcagcc gcccacccca atcagcagcc gggggttggc gcgcttgtag tgcttgacgc
     2401 gcccgaggtc ggcgaacacc ttctcctgcg ccttctcgcg caccgagcag gtgttgaaca
     2461 ggatcacatc ggccgcggcg gggtctgcgg tcggctccat gccgtgggca aggcgcagaa
     2521 cgtcggccat cttgcccgag tcgtactcgt tcatctggca gccgaaagtc ttgatgtaca
     2581 gcctggaggg cacgacgcga aacggtaaaa agcgcgggtc agcgagcgcg ctggagctgc
     2641 tccagctcct cgggcagcaa caggtatatg cgcgagacgt cgccgttcat gtcctcgacg
     2701 aacagcacgt aggcatgctc cgggagggtg ccgtgcaata tcgtacggtt atgctggtcg
     2761 atgatgcgcc cgcccggtga gagacggagt atcttgttgt tgatttgaag gagcgggaag
     2821 ggctgttgcc ctccgatcaa ctcgccgagc ttgccgtttg ccggcaactg gcggggcagt
     2881 tgagcgtgac tcgtcgcggc ccatgctgct gccagcgcca ggatccaggc ccatcgtcgc
     2941 atcgattcac agtaacacaa cgtaatcaaa tacttcaacc gtgatctgga gccgttttcg
     3001 aacgcaggcg gcaaccgccg caatctgctt aaaactggtg gcgagtcagg gatttgaacc
     3061 ccggaccaac ggattatgat tccgctgctc taaccgctga gctaactcgc cgtaggaagc
     3121 cggcgagtct aaagagcggg gccggcattg tcaagttaag ccactgaatt tctgccgaat
     3181 tcctgttaaa tatccggtgc ttgtagtagt ccaatggaaa gtgctatgct aattttgaag
     3241 tgcgaatcag tattccacat cacgaaattt acaacttgag tcagacggcg aaggtacgga
     3301 agtcgtaatc cgggttcgct ggttccacct gttttactac tcgacacgaa gaggaggttt
     3361 tgcgtgcgca ccccttacaa aattctgatc atgggcgcct cttacggctc gctgttggcg
     3421 gccaagctgc tgttcgcgcg ccatacgctg aagctggtct gcctgcccgc cgagtcggaa
     3481 ttgatcaaca aggaaggcat ccgggtacgg ttgccggtga aaggcaggga ggggctcgtc
     3541 gagatcgatt cgcggaaact gccgggcaag ttgtcggccg atggtccgca ggcggtcaat
     3601 ccggccgact acgatctcgt cgcgctggcc atgcaggagc cccagtatcg ttcgcccggc
     3661 gtgcgcgagc tgctggatgg ggtggccaga gccagggttc cgtgcatgtc aatcatgaac
     3721 atgccgccgc tgacttacct gaaacgcatc ccgggcctca atgcagatgc atgcagaggc
     3781 gcttataccg accccacggt ctgggatggc tttgatccca agctgatgac gctgtgcagc
     3841 ccggacccgc aggcattccg cccgccggag gaaaaagtca atgtgctgca agtcgggctg
     3901 cccaccaact tcaaggcggc gcggttcgat tcggaagctc acaccgccat cctgcgccag
     3961 ctggaggcgg acattgaagc ggtgcggttt gacaccggtt ccggcaagat cgagctgccc
     4021 gtcaagctca aggtgcacga ttccgtcttc gtgccgctgg cgaagtggcc catgttgatc
     4081 gccggcaact atcgctgcgt gcagaaagac gccatgcgtt ccatcaagga aaccgtgcat
     4141 gccgacatcg aaacatcgcg ggcggtttac aactgggtgg tcaagttgtg cacgtctctc
     4201 ggtgcggatg agaaggacct ggtgccgttt gaaaagtacg ccaacgccgc gctatcgctt
     4261 ggcaaaccgt cctccgcggc gcgggccttg ttcgggggcg cacccaatat cgagcgggtg
     4321 gaccgtctgg tgaaggccat tgcggcgcag aagggcatgc agtccagggt gcttgacgag
     4381 acggtcgccc tggtcgacgc ccggctggag accaatcgga agaaggcggc ctgattcagc
     4441 gccacgcgtg cagttggtca aagcgcggaa gcaagcgtag tcaggccgca accacggcgt
     4501 taaactgcgc gggccatcat gaaagccgtc cacgcatgcg ccaagctttc gatatcgtca
     4561 tcgtcggcgg cgggctggtc ggcttgagcc ttgcgcgagc gctcgcccgc agcgggctct
     4621 ctctggctct cgtcgaacct cagccgctcg caccgattcc cgaagacggc tcgtgggaca
     4681 gccgcatcta cgcggtgagc cccggctgcg ccgcgttcct cgagcgttgt ggcgcgtgga
     4741 atcggttgcc gcaggagcgg gtgacgcgag tcgaggccat gcgcgtctac ggcgatgacg
     4801 cagacgcccg tctcgagttc gatgcctatg acgccgggct gcgcgaactc gctttcatcg
     4861 tcgaaagccg tcagctgcaa cgcgcgctgt gggaaacgct gtttagccag gatgcccgcc
     4921 tctattgtcc cgccaactgg accgccctcg agtttcaggc cgatcatgcc gtgctgcggc
     4981 tcgatgacgg caccgaactc gcggcgcgcc tgctggtcgg cgccgatggt tcggagtcgc
     5041 gggtgcgtgc ggaagcggga atcacggtga cgcccaccga ctaccgacag ctcgggctgg
     5101 tcgccaattt tttctgcgag aagccgcacc gcgacaccgc gttccagtgg ttcatgcgcg
     5161 acggcgtact ggcgctgctg ccgctgccgg gcaaccgcgt ttcgatggtg tggtcggttg
     5221 cgcaggagcg cgggcggagc ctgcttgcgg cgccggccgg ggaactcgcc acggaagtcg
     5281 agtcggccag ccgtggcgcg ctcggcgcgc tgcggctcat gaccccggcc gtcgcattcc
     5341 cgctcaagtt gcagcgcgtg agacggttcg tgaagccgcg ccttgcgctg gtgggagacg
     5401 ccgctcataa tgtgcacccc cttgcgggtc agggagtgaa cctcggcttc cgggacgctc
     5461 gcgtgctcgc tggagtgctg gcggaacgcg ggacggagcg cgactgcggc gattacgcgc
     5521 tgctccggcg ctacgagcgc gcgcgccggg aggacgtgct cgccacgcag ctcacgaccg
     5581 acggcctgca gaaacttttc aacaacgacg ctgtcttgct cacgagggcg cgcaacctgg
     5641 ggctgagact ggtcaactgg cagccattgc tcaagaacct gctggtgcgg cacgccgtgg
     5701 cgtgacgcca tacgacgaag gaacgacatg tatcgcagga tcgcaatctg cctgctgggc
     5761 attttcggcc ttgccaccgg catggcgggc gcagacgaaa accgggtgag acaactgctg
     5821 caggcgaagt ttccgatgat gaaagtcgag agcgtcgtca ggacaccgta cagcgggctg
     5881 tacgaggccg tgctcgacgg cgaaatcgtc tataccgacg agggcgcgac cttcctgttc
     5941 ggcggcaata tctacgacgt gcgcacgctg ccgccgcgca acgtgaccca ggaacgcacc
     6001 gagcaactgg cggcgaaaat actcactgac gcgcgtgaaa tggcgatcaa gcgcgtcagg
     6061 ggcggcggcg cgcgcacgct gttcactttc gaggacccga actgcgctta ctgcaaggcc
     6121 ctgcagaagg agctggccaa ggtcgagaac gtgaccatct acaccttcct gatgccggtg
     6181 ctttcgcagg attcggtcga gaaatcggtc gccgtgtggt gcgccacgga tcgcgccaga
     6241 gcgtgggacg agttgatgtc aaacggcatc gtgcccgaaa acgacaaatc ctgttcgcac
     6301 ccgcttgcgc ggatctccgc catcgcgcgc cgcttccaga tccagggcac gccggcgatt
     6361 ttcctgggcg acggcaagca gatcggcggc atgcgcagcg ccctggacat cgaaaaggcg
     6421 ctcagtgcgg tcaagtaacc gcgcgcacgc gccgatcctg attcgcgcgc tgcgggcgtt
     6481 gcttcccgtc ctggctgcgg cgtggacgtg ttcggcactc tccgcccagg acatcgaatc
     6541 cggcatccgg cgggcgtttc aggcgaagtt tcccaagtcc gcggtggaaa gcgtcacgcg
     6601 cacgccgttc accggtctct acgaagtggt gttcaacggg cagatcgcct ataccgacga
     6661 gaagctcaat ttcgtctttt tcggcaatct ctacgatgtt cgcagcggcg tcgagcgcaa
     6721 cctcacgcgc gagcgcagcg cccagctcgc cgcccagacg ctcaggaagt caaccgactc
     6781 cgcgatcaag cgcgtgcgcg gcaacggcag gcgcgtgctc tacaccttcg aggaccccga
     6841 ctgcccctac tgcagggagc tgcagaagga gctgctcaag ctgagcgatg tcaccatcta
     6901 taccttcctg tggcccattc tctcgcccga ttcggtggaa aagtcgaagg cggtatggtg
     6961 cgccaaggac cgcgccaagg cgtgggacga tctcatgtcg cgcggcgtgg cgccgccggg
     7021 cggggccaag tgcgataccc cgctcgaaaa gaatgccgaa ctcgcacagc gtttcggcgt
     7081 acgcggcacg cccgcggtct atcttgctga cgggaacatg atcggcgggc tggttccggc
     7141 cgacaagctc gagcaggcgc tcaacacagt tcactcccgg taggaattcg ccgaacgggt
     7201 tccggcgaat tccttaacga aaaccacttc gagcaactgg ttcgaaaggc tgccggtgcc
     7261 ttccgggtgc cctccttttt tgcggacggt ggtgaagcag cgtcttgtcg acaggtagcg
     7321 caagtcggat ggagttctgg ttatggaagt ctgccggact gccgtccagc agacttccat
     7381 gtgtcaacag gggataacgg cagccgttat cccccttatg acgagcgcgg ttctttcccc
     7441 ttcttccccg ccccgcctgc aggccgctgg ccgtttcggg ctggcgctca tggcatccgc
     7501 ggcctttcat ttcctgctgc ctgtggtcat gatgtccgat acgccgtggc ggagcgcgcg
     7561 gcaaggaatc gctgctccga tcacggtgcg actcgaatcc cagccgtgga tggcgtcagc
     7621 caacccgacc cctatcgata gtaaagaacc ttccgggccg cgtccgacga gacgccctgt
     7681 cgcggttccc gaagacgtac gacacaaggc ggcgcctggg ccggattcgg gaaagacacg
     7741 ggacacggcg ggcccgcttg cattgccgca ggtcccggac ccgacctatt attccgcgcg
     7801 cgatctcgat gtttacccaa gaccgattgt gccgctcgag ctcgatcggc tggcgggcgg
     7861 cgttccagga gaggctgcgg ccagattcag gctcgtactg ctcatcgacg agcgcggcat
     7921 cgtgagcgag atcgccgtcg tcgaggccga tccgccgggc cgccttgagg aggcgttgcg
     7981 cgccaggctc gcggcgaccg gtttttttcc cgcgatgaag gacggccgcg ccgtgaagag
     8041 ccgagtggag ctgagcgtga ctattggccg gaaaggcgct gaccggcaac ccgagaagag
     8101 cgtgagtggc gagtggtgag tggcggtacg aagaaaaacc gtctcgtcga atcacgattc
     8161 acgaatcaca cttttccgtc gctctcacgg cgcccggaaa ttcactcctg gctgtttggc
     8221 acgcgaaagg cgttgggcag cagcacccac atgcgcaaca actgacgcat ccgcccggcc
     8281 tcgcgcgcgg cttcctcgcc gaagccccag aaaaagtcgg cgcgcaccgc gcctcgaatc
     8341 gcgccgcccg tatcctgcgc cagcatgagg cggttgagcg gccggctgga aagcggccag
     8401 gtggtcgcaa ggtagaccgg ggcgcccagc ggcacgaaac ccgggtccac cgccacgctg
     8461 cggcgcgggg tgagcggcac gccgagcgcg ccgggcgggc ccggcgtatc ggccggcagc
     8521 tcgcggaaga acacgtagct cgcgttctgg ttgagcagct cggtcacccg ctcggggttc
     8581 gcgcgcgccc acgccttgat gccctgcatt gaggcctttt ccagcggcaa ctcgccgctc
     8641 tcgaccagga acctcccgat cgagcgatag ggatagccgt tctggtcggc gtagccgacg
     8701 cggagcgtct cgccgttctc gagcgcgatg cgacccgagc cctggatctg gaggaagaac
     8761 agctcgattg cgtcgtccac ccagacgatc tccttcccgg tgagcgcgcc gcggccctgc
     8821 tcgatctgcg cccggtcgta gtagggcacg atgcgccgcc cttcgagccg gccgcgcagc
     8881 cgcatgccct tgagctccgg atacacgtcg gtcagatcga ccaccagcag gtcgtccggc
     8941 aatccgtaca ggggatggcg gtagcgcgcc gatggtttac ggcttcctct gagcagcggc
     9001 tcgtaatagc cggtgatcag accttcttcg ctgccgtccg cgttgctcaa ctgaaatggc
     9061 gtcaggttgg actcgaaaaa gcggcgcgtc atctcgcgat ccgggctctt gatgctttcg
     9121 gccactacgc agatgtcacg ccaggcgtcc tgcttggcga gcgcccggca gcttacaaga
     9181 aacgcgtccc aggcctcgcc cgggtcttcg gcctgccatc cgggaagatc gctccaggaa
     9241 acctggcgaa gcaggcccgc cgtgccggcg ggtggaggct tcatcggtgt gatttccgcc
     9301 ggtggcgggg gcttcggcgc ggggggcggg ggggccggtt gtggcttgag ggtttcgcaa
     9361 cccgccaagg caagcagcgc gcacaaggaa aacacgtgca cgcagcgtac ttggtcttgg
     9421 aacggcaatt cgctagagtg cgacatcatg aatccggtga gcagtaaaca gtgagcagtg
     9481 agcgggaact tcctgaagaa gcgccgccca attttaccgc ttaccgctta ccgcttaccg
     9541 cttaccgctt accgcttacc gcttaccgct taccctatgg cagtataagc aatggatggt
     9601 ttcctcctgc tgctgctgga agcgggggtc gcgctcgccc tgctcgtatt catcgtgtgg
     9661 tggacctggc cgcggcgcag gccgagcgac gacgacgatc gggcgggcag ggatggaaag
     9721 cgctgaccct cacagtctct tcagcctgag caggcgaaac cagcccctgg cggcggccgg
     9781 tgtatcgctc gtcacccgca cgccgggcac cgccggcaac acatcatcca aaaccacgtc
     9841 gagtcgagtc accagccgcg ccgcgagcgg gctgagcagg cggcgcaagg gggcgcgttg
     9901 attgcccttg aggaatttgt cgaacaccag aatactgccg cccgcgcgca caacgcgcac
     9961 ggtttcggag agggcgcgga ctgcatccgg caccaccgcc aggatcagat gcaataccgc
    10021 gtggtcgaag cttgcgtcgc ggaaaggcag ggtcagactg tcgccttgca ccaatacgaa
    10081 ctcacgcccg tcggcccggc gcgcgcaacg ccggagcatg gcgcgcgtca gatccagccc
    10141 gacgtaacgg tggcagcgcg gcaggaacgg aaaatcaagg ccggtgccga caccattcaa
    10201 aagcacggtg gcatgaccct cgcgcggcag ggcggcgagg cttgcgcggc gggccgcacg
    10261 aaaagccggt cgcgcgacga ggtcataaag aggcgcaagg agggtgtagc tgatccgcag
    10321 gctcatcgag attcaagtgt tgagttttta gtgttgaatg ctgagttata acgtcgtccc
    10381 gcccactcaa cactcaacac tcaacactca acactcaaaa ctcatccctc acgtcagtgg
    10441 aggacgcgcg gcatggagag cgtgaactcg ggtatcgcag cgtcgaactg cacgccgtcc
    10501 tccgccatca tctggtagct gccgcgcatg gtgccaaccg gcgtggcgat cgcagtgccg
    10561 ctcgtgtact ggaagctctc gttgggacgc agcaacggct gctcgccgac cacgccgagc
    10621 ccgcgcactt cctgcacttg gttgttggcg tcggtgatga tccagtgccg gctcaccagc
    10681 tgcgccgaca ccgtgccggt gttggtgatg gtaatggtgt aggcgaacac gtaacgcccc
    10741 ttccgcaggt ccgactggtc cgggatgaaa gcggtgcgcg cttcaaccac gatgtcgtac
    10801 ttcttgtccg tcataccgtc gattcttgat catttcaccg ccgcgcgcaa taacgggtag
    10861 aatccccgcg tcgtcgagga acgtgcatgg cgaaaaccta ccgcatcgct cccagcatac
    10921 tgtcggccga cttcgcccgg ctgggcgaag aagtgcaaaa agtggtcgcg gccggcgccg
    10981 acctcgtgca tttcgacgtg atggacaacc attatgttcc caatctcacg gtgggcccgc
    11041 tggtgtgcga ggcgatccgg ccgctcaccc gcgccatcat tgacgttcac ctgatggtga
    11101 agccggtgga ccgcatcgtt cccgacttcg ccaaggccgg cgccaacatc atctcgttcc
    11161 accccgaggc ctccgagcac gtggaccgca ccatcgcgct gatcaaggac cagggctgca
    11221 gggcaggtct cgtgttcaat cccgccacac cgctctcgca tctcgaccat gtcatcgaca
    11281 agctcgacct cgtcctcgtc atgtcggtga atcccggctt cggcgggcag aaattcatcc
    11341 cccaggcgtt gaagaagctc gccgccgtgc gcacccgcat cgaggcgacc ggacgcgaca
    11401 tctggctcga ggtggacggc ggcgtgaagg tggaaaacat cgcggaaatc gcgcgcgccg
    11461 gggccgacac cttcgtcgcc ggctccgcca tcttcggcgc caaagactac aaggccacca
    11521 tcagcacgat gcgcgccgag ctcgcgaagg cgtagaagga ctcgacgcga agccgcaaag
    11581 tcgcaaagaa agccggtgca agattttcga catcaagacc gatacgtcga agaccgtgat
    11641 accgctcgcc atcgggtttt ccaagccgca gcgatggttt cggcctggat cttgatgaat
    11701 tcctttgcgg ctttgcgcct ttgcgtcaag ttttgactac tcagcgcttt ccgctgcgcg
    11761 tcagggcggt catgatcgac ctcgacggca cgctcgccga caccatcccg gatctcgcgg
    11821 ccgcggccaa catgatgctg cgcgagctgg ggcggcccga gctcgacgaa agcctgatcc
    11881 gcaacttcgt cgggaagggg ataccccggc tggtggagcg ggcgctcgcc ggcggcattg
    11941 acggcaccgt gccggcgcca atgctcgagc gcgccctgcc ggtttacgag cgctgctacg
    12001 ccagcgtgaa cggcaggcgc acgacgatgt ttcccggcgc aagggaaggg ctcgaagccc
    12061 tgcgcgcggc ggggttcccg ctcgcgtgcg tcaccaacaa ggcggagcgt ttcacgcggc
    12121 cgctgctcga cggcatgggg
//
LOCUS       DBNGWZ010000411        12474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2066296, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000411 DBNGWZ010000000
VERSION     DBNGWZ010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2066296"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16CX_12240"
     CDS             <1..391
                     /locus_tag="AC16CX_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3013315.1"
                     /translation="ELGLTGTHVGCDTSQCGCCTVHVNGRAVKSCTMLAAQANGADVV
                     TIEGLAKKGKLHPIQRAYSECHGLQCGFCTPGMIMATAGLLKDKPNPTEEEIYKGLEG
                     NLCRCTGYINIVKAVKRSAKLLRGKAR"
     gene            680..1672
                     /locus_tag="AC16CX_12245"
     CDS             680..1672
                     /locus_tag="AC16CX_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013316.1"
                     /translation="MKKLTIAALVLAGAGITTTDTLAQGTAADYPTRPIRVLVGFPPG
                     GATDILARILAGHLASSWGQQVVVDNRGGAGGTLAASIAAKATPDGYTLMMVPSGPFT
                     ISVSTYEKLPYDAVKDFAAISLLAWVTNAIVINQASPVQTLQDLIRLAKEKPGQVTNG
                     SSGNGSLHHLAGEVFKRLTGTNIIHVPFKGGGPMIVAVAANEVTFAFASVPSAMPLIQ
                     GKRIRPLVVTSLKRSTALPDVPTIAEAGVPLPAGLEMREWYGMLAPARTPPAIVDKLN
                     AEMVKIFRRPDVQTRLNEMGAELAGSSPKELAAQIASDVKTWAAVAKEAGVRAD"
     gene            complement(1695..2405)
                     /locus_tag="AC16CX_12250"
     CDS             complement(1695..2405)
                     /locus_tag="AC16CX_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013317.1"
                     /translation="MPETPLIEARALQTYYGASHILHGVSLSVGRGETIGLMGRNGMG
                     KSTLIRTLMGLVRPRKGSVLVNGRDMSAAPTHEIARQGIAYVPEGRAIFPNLSVRENL
                     VMAARPGRRRDWTYERVMRTFPRLAERRNHGGGQLSGGEQQMLAIGRALMTNPELLIL
                     DEATEGLAPLIAREIWSIIREIRESGMATIIVDKNHAAVTAVSSRNIILVKGRIVFEG
                     TSDALRAQPGVLHRHLGV"
     gene            complement(2398..3156)
                     /locus_tag="AC16CX_12255"
     CDS             complement(2398..3156)
                     /locus_tag="AC16CX_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013318.1"
                     /translation="MADATLRISGLSKRFGGVVAVDDVTLEIAGNAVHAVIGPNGAGK
                     TTLANLLSGDLRPSAGRITFEGAEISHLPADTRSRMGIGRSYQKTNVFREFTALENCR
                     LAAQSRNPRPYNWFRDALSYRDVTAAAEKALATVGLSARTTVIAGHLSHGEQRQLEIA
                     MCLATNPRVLILDEPLAGMGAEESAAMVALLRTIAADHAVLLVEHDMDAVFALAAVLT
                     VMVNGVVLDTGAPEKVRANPDVQNAYLGASEDHA"
     gene            complement(3149..4141)
                     /locus_tag="AC16CX_12260"
     CDS             complement(3149..4141)
                     /locus_tag="AC16CX_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3013319.1"
                     /translation="MQPAPKITRVLLLLGLASLALFPLAGSTFYIQLFTKIMIMAIFA
                     MSLDLIVGYTGLVSLGHSAFFGFAGYLLALTAPKYETVSLWLTLPACMAATGVLALAI
                     GVLVLRTSGIYFIMVTLAFAQMLYYLFHDTKLGGGSDGIYLHARPSLAIGGFNPLDLG
                     DHAHFYYLVLALMLAVYLLLRQVLASPFGRVLVSIQANEARVRALGFQVFNYKLGAFV
                     LAGTLAGLADYLAACQFGFVNPEILSWHYSGVVLMMLILGGMGRLYGAVIGAFAFVLV
                     QEFFSYQDWFGDYARRWQLAMGCFIVLVVLLLPQGLADLLERLGRKPAPEPRNG"
     gene            complement(4141..4998)
                     /locus_tag="AC16CX_12265"
     CDS             complement(4141..4998)
                     /locus_tag="AC16CX_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3013320.1"
                     /translation="MEFLIQILNGIQYGLLLFLLASGLTLIFGIMGVINLAHGSFYMV
                     GAYLAYWLVAETGSLWLALPIGVVISLVIGVLLESALIRTLYERDHLYQVLLTFGLIL
                     IFEELRSILFGNDVHGVPVPAVLDYSIPLTETLSYPVYRLFISGVCLALAAALYFVIQ
                     KTRIGMMVRAGNSNREMAQVLGVNIPLIYSLVFALGVALAAFAGMIAAPVSSVFPGMG
                     NQILIICFVVVVIGGIGSIKGAMVAALLIGIADTLGKVLLPEYSGMAVYLLMAAVLIW
                     RPQGLFQAT"
     gene            complement(5116..6297)
                     /locus_tag="AC16CX_12270"
     CDS             complement(5116..6297)
                     /locus_tag="AC16CX_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013321.1"
                     /translation="MTIYRRGFLQTLGTLALGSAIPAGPLFAQQPKLRIGLMLPYTGT
                     YAALGTSITNAFKLAVAERGGKLGGREIDYFTVDDESDPAKAPENTNRLVQRDRVDVV
                     VGTVHSGVLMGMVKVVRETGTLLVCPNAGAGDATGAACAPNIFRTSFSNWQTTYPLGK
                     VLTDKGLKTAVFITWNYAAGKEMLDGFKEAFEKRGGRLVKELLLPFPQVEFQALLTEA
                     AATKPDAVVAFFAGGGAAKFLRDYAAAGLKARIPLYGSGFLTDGVLDAAGDAADGVET
                     TLHYGDGLETPRNNAFRVAYVRAYKSPPDVYAVQGYDAALLLDVGLNAVKGDVSRKKE
                     LIAAMEKAKIDSPRGAWTLSKAHNPVQDFYLRKVIGKENKIVGVAHKALADPARGCKM
                     S"
     gene            complement(6332..7927)
                     /locus_tag="AC16CX_12275"
     CDS             complement(6332..7927)
                     /locus_tag="AC16CX_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008652169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HIR3013322.1"
                     /translation="MPGLSSADHRAHPPVVSYPRDYNAAHDLIERNLRAGRAGNIAYI
                     DDGGSYSYGELAERVNRWANALTGLGLQMDQHVLLCLLDTIDFPTAFLGSIKAGIVPI
                     AANTLLTSGDYEYMLKDSRARALVVSEPLLPVFAPLLGRFPTLKHVIVSGRNSAGHLS
                     LSGLLAQSSAAFDAAPTTADDACFWLYSSGSTGLPKGTVHVHTSMILTAELYARAVLG
                     IQESDVVFSAAKLFFAYGLGNALSFPLAVGATAVVMAERAAPPAVFARLRKHQPTIFY
                     GVPTLYAAMLANPELPRREELRLRRCVSAGEALPQDIGRRWSDCFGVDILDGLGSTEM
                     LHIFLSNRPGDVRYGTSGKPVPGYALRVVDENSADVAPGQQGELLVSGPTSAGYYWNN
                     RERSRSTFLGAWTRSGDKYTVNEQGYYVYAGRTDDMLKVSGMYVSPVEVESALITHQS
                     VLEAAVVGKEDDQKLVKPLAFVVLKPGAEGSEQLAEELKQHVKARLAPYKYPRWIEFV
                     SDLPKTATGKIQRFKLRELAARR"
     gene            7998..9656
                     /gene="boxC"
                     /locus_tag="AC16CX_12280"
     CDS             7998..9656
                     /gene="boxC"
                     /locus_tag="AC16CX_12280"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HIR3013323.1"
                     /translation="MATQPPVTYETHPDRYLHWRLSFDGPVATLTLDVDENRGFRPGY
                     KLKLNSYDLGVDIELHDALQRIRFEHPEVRSVILTSGKDRIFCAGANIFMLGQSSHAW
                     KVNFCKFTNETRNAIEDASAHSSLKFLAACNGTTAGGGYELALACDEIVLIDDRSSAV
                     SLPEVPLLGVLPGTGGLTRLTDKRKVRRDLADLFCTTAEGVRGARAKEWGLVDDVVKP
                     QQFAGQVKHRALELAARSDRPRDAQGVTLAPLERRVDGAGYHYPFIDVRIDRGARHAT
                     ITVSAPDAPQPQDLEGILARGAGWWPLRVARELDDAVLMLRTNDLDIGIWLLKTRGSA
                     DHALAVDASLGRHRDHWFVREVTGMLRRTLNRLEVSSRSLFALIDAGSCFAGTLLELA
                     LAADRSYMQQRVAEGAGAPVIALSPMNFGAYPAVNRVNRLESRYYGDAGQVAAARRAV
                     GRKLAAGEALELGLITVAPDELDWSDEIRLALEERASLSPDALTAMEANLRCGGAETT
                     ETRIFGRLSAWQNWVFSRPNAVGDAGALKVFGTGNKPRFNWERV"
     gene            9692..11116
                     /gene="boxB"
                     /locus_tag="AC16CX_12285"
     CDS             9692..11116
                     /gene="boxB"
                     /locus_tag="AC16CX_12285"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HIR3013324.1"
                     /translation="MTIDYSAKIPNNVDLSGDKALQRALERWQPAFVGWWGEMGPEAA
                     RGCDVYLRTAIGVDRDGWANFGYVRMPDYRWGIFLNPGEPGRTIGFGDHKGEPAWQEV
                     PGEYRSNLRRIIVTQGDTEPASVEQQRRLGFSCPSLYDLRNLFQINVEEGRHLWAMVY
                     LLHRYFGRDGREEAEALLERRSGDRDNPRILGAFNEATPDWLSFFMFTFFTDRDGKFQ
                     LTALAESGFDPLARTTRFMLTEEAHHMFVGVTGITRILQRTAQVMAQHKTDDPARLRA
                     LGVIDLPTIQRYLNFHYSITLDLFGSDVSSNAATFYTTGLKGRFDEARLDDDHRLKDL
                     TYPVPEVAGGRIVQKEAPALNAVNERLRDDYIQDTLAGVARWNRVVQKGGVGFQLQVP
                     NKGFNRRIGSFAGLRISPDGRVVSEKEWEANVAAWLPTAEDRAFVQSLMGRVVEPGKF
                     ANWIAPPERGIDGQPVNFEYVRFN"
     gene            complement(11195..11596)
                     /locus_tag="AC16CX_12290"
     CDS             complement(11195..11596)
                     /locus_tag="AC16CX_12290"
                     /inference="COORDINATES: protein motif:HMM:NF017913.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HIR3013325.1"
                     /translation="MIERIEAFGGVLVKGFHLLALFVIGGTVVWSAAHFYLVEIMQRG
                     AAKLHDILLLFIYLEIGAMVGIYFKTHRLPVRFLIYVAITALTRMLTIDIKDLNNEQI
                     LTATGAILVLALAALAVDFSAARFRSPDEDV"
     gene            complement(11927..>12474)
                     /locus_tag="AC16CX_12295"
     CDS             complement(11927..>12474)
                     /locus_tag="AC16CX_12295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3013326.1"
                     /translation="RHDYEVAGSACINWLEKRADVDPRKIGIVAISLGGYYAPRCAAM
                     EPRFAACIAWGAQWDYHDTWKKRVAASFKTALSVPGHHIEWILGAASLDEALKKLEPF
                     RLDGVMQKMRCPFLLVHGADDEQIPLTDAQKQFDACGSRDKTFRVFTAAEGGAQHCQR
                     DYLTLVVAEMWNWFEEKLVRA"
BASE COUNT         2147 a         4050 c         4278 g         1999 t
ORIGIN      
        1 cgagctgggg ctcaccggca cccacgtcgg ctgcgacacc agccagtgcg gctgctgcac
       61 cgtgcacgtc aacggccggg cggtgaagtc gtgcaccatg ctcgcggcgc aggcgaacgg
      121 ggcggacgtc gtgacgatcg agggcctggc gaagaagggc aagctccacc cgatccagcg
      181 cgcctactcc gagtgtcacg gcctgcagtg cggcttctgc acgccgggca tgatcatggc
      241 gacggcgggg ctcctgaagg acaagcccaa ccccaccgag gaagagatct acaaggggct
      301 ggagggcaac ctctgccgct gcaccggcta catcaacatc gtgaaggcgg tgaagcgcag
      361 cgcgaagctg ctccgcggca aagcgcgctg acccgacgca gcagccgagg gcggcacgtc
      421 agcaatccac ttggctggtg tcatacccgc ggaattggtc cgcgcgcttt gtaaacccgc
      481 ctgcggcggg ctgcgcttta caacgatatt cgcgggggaa aaaagcgcct cggggcagcc
      541 gggctgcggg aaggagcttt ccgcgccttc gcggttggta tagattcctt ggttccaggg
      601 ctaccaagcc ctcgaggagc gcggcaggat acgcgaggcc gcggtgaagg gcggcgtgca
      661 gctgccgagg aggaggatga tgaaaaaatt gacgattgcc gccctggtgc ttgccggcgc
      721 cggcatcacg acgaccgaca cgcttgcgca gggcacggcg gcggattacc cgacgcggcc
      781 gatccgtgtc ctggtcggct ttccgcccgg cggtgccacc gacatcctcg cgcggatatt
      841 ggccggccat cttgcgtcgt cgtggggtca acaggtcgtg gttgataacc gcggtggcgc
      901 cggtggcacc ctcgcagcca gcatcgcagc gaaggcaacc ccggacggct ataccctgat
      961 gatggtgccc agcgggccgt tcactatcag cgtcagcacc tacgagaaac tgccctacga
     1021 cgcggtgaag gatttcgcgg cgatttcatt gcttgcgtgg gtaaccaacg cgatcgtcat
     1081 aaaccaggcc tctcccgtgc aaacgctgca ggacctgatc cgtctggcaa aggagaagcc
     1141 tggccaggta accaatggct cgtcaggcaa cggctcgctg caccaccttg cgggggaggt
     1201 gttcaagcgg ctcaccggca ccaacatcat ccatgtgccc ttcaagggcg gcggcccgat
     1261 gattgtggcg gtcgccgcca acgaggtgac gttcgcgttc gcgtcagtgc cctcggcgat
     1321 gccgctcatt cagggcaagc gcatcaggcc gctcgtcgtg accagcctga agcgctcgac
     1381 agcgctgccc gacgtgccga cgatagcaga agccggcgtg ccgttgccgg cggggctgga
     1441 aatgagggag tggtatggca tgctcgcacc ggcgcggacg ccaccggcca tcgtggacaa
     1501 gctcaatgcc gaaatggtga agatcttcag aaggcccgat gtgcagacgc ggctcaacga
     1561 gatgggcgcc gagctcgcgg gcagctcccc caaggagctg gcggcgcaga tcgcctccga
     1621 cgtcaagacc tgggccgcgg tggcaaagga ggccggcgtg cgtgccgact agcctgacta
     1681 tttcatgaag gcgttcaaac acccaggtgc cggtgcagga cgcccggctg cgcgcgcagc
     1741 gcgtcgctgg ttccctcgaa gacaatccgc cccttgacca ggatgatgtt gcgggagctg
     1801 accgcggtga cggccgcgtg gttcttgtcg acgatgatcg tggccatgcc gctttcccgt
     1861 atttcacgga tgatcgacca gatctcgcgc gcgatgagcg gcgccaggcc ttccgtcgcc
     1921 tcgtcgagta tcagcagttc cgggttggtc atcagcgcgc ggccgatggc gagcatctgc
     1981 tgctctccgc cggagagctg gccgccgccg tggttgcgcc gctcggcgag gcgcgggaag
     2041 gtccgcatga cgcgctcgta ggtccagtcg cgccggcggc ccgggcgcgc cgccatgacg
     2101 aggttctcgc gcacgctcag gttggggaag atggcgcgac cttccggcac gtaggcgatg
     2161 ccctggcgcg cgatctcgtg ggtcggggcg gcgctcatgt cgcgcccgtt gacgagcacg
     2221 ctgcccttcc gcgggcgcac caggcccatg agcgtgcgga tgagcgtgga cttgcccatg
     2281 ccgttccggc ccatgaggcc gatggtctct ccgcggccca cgctgaggct gacgccatgg
     2341 agaatgtggc ttgcgccgta gtaagtctgc agcgcgcgcg cttcaatgag cggggtttca
     2401 ggcatggtct tcggacgccc cgaggtaggc gttctgcaca tccggattgg cgcggacttt
     2461 ctccggggcg ccggtgtcga gcacgacccc gtttaccatc accgtgagca ccgcggccag
     2521 cgcgaacacg gcgtccatgt cgtgctccac cagcaatacc gcgtggtcgg cggcgatggt
     2581 cctcaacagc gcgaccatcg cggcggactc ttccgccccc atgccggcga gcggctcgtc
     2641 caggatgagc acgcgggggt tggtggcgag gcacatggcg atctccagct gccgctgctc
     2701 accgtggctg aggtgcccgg cgatgaccgt cgtgcgcgcg ctcagcccca cggtggcaag
     2761 ggctttctcc gcggccgcgg tgacgtcgcg ataggaaagc gcgtcgcgga accagttgta
     2821 cgggcgcggg ttgcgcgatt gcgcggcgag ccggcagttc tccagcgccg tgaattcccg
     2881 gaacacgttc gttttctggt agctgcggcc gatgcccatg cgcgagcggg tgtcagccgg
     2941 caggtgcgag atctccgctc cttcgaacgt gattctcccc gcggacggcc tcaggtcccc
     3001 ggagagcagg ttggcgagcg tggtctttcc cgcgccgttc gggccgatca cggcgtgcac
     3061 ggcattgccg gcgatctcca gcgtcacgtc gtcgacggcg acgacgccgc caaaccgctt
     3121 gctcaggccg gaaatgcgga gcgtggcgtc agccattgcg cggctccggc gccggcttgc
     3181 ggcccaggcg ctccagcagg tcggcaaggc cctggggcag cagcagcacg acgagcacga
     3241 tgaagcagcc catggcgagc tgccagcgcc tggcgtagtc gccgaaccag tcctggtagg
     3301 agaagaattc ctgcaccagc acgaaggcga aggcgccgat caccgcgccg tagagccggc
     3361 ccatgccgcc cagtatgagc atcatgagca ccacgccgga ataatgccag gagaggatct
     3421 ccgggttgac gaagccgaac tggcaggcgg cgagatagtc ggcgagaccg gccagcgtgc
     3481 cggccagcac gaatgcccca agcttgtagt tgaagacctg gaagccgagc gcgcgcacgc
     3541 gcgcttcatt cgcctggatg ctgacgagca cccgtccgaa cggcgaagcc agcacctggc
     3601 gcagcaggag gtagacggcc agcatgaggg cgaggacgag gtagtagaaa tgcgcgtgat
     3661 cgccgaggtc gagcggattg aacccgccga tggcgagcga aggcctggca tggagataga
     3721 tgccgtcaga accgccgccc agcttggtgt cgtggaacag gtagtagagc atctgggcga
     3781 acgccagcgt gaccatgatg aaatagatgc cggaggtgcg caggaccagg acgccgatgg
     3841 cgagggcgag gacgcccgtt gcggccatgc aggcaggcaa cgtcagccac aggctcaccg
     3901 tttcgtactt gggggccgtc agcgcgagca ggtatccggc gaaaccgaag aacgcggagt
     3961 gcccgaggct caccagcccg gtgtagccga cgatgaggtc gaggctcatt gcgaagatcg
     4021 ccatgatcat gatcttggtg aagagctgga tgtagaacgt cgagccggcc agcgggaaga
     4081 gcgcaagcga cgccagtccc aggagcagga gcacgcgcgt gatttttggg gccggttgca
     4141 tcaggtcgcc tggaacagtc cctgcggccg ccagatcagc acggccgcca tcagcaggta
     4201 taccgccatg ccggaatact cgggcagcag caccttgccc agggtgtcgg cgatgccgat
     4261 caggagcgcc gcgaccatcg cgcccttgat cgagccgatg ccgccgatca ccacgaccac
     4321 gaagcagatg atcaggatct gattgcccat gcccgggaac accgaggaca ccggcgccgc
     4381 gatcattccg gcgaaagcgg cgagcgccac acccaacgcg aacaccaggc tgtagatgag
     4441 cgggatgttc acgcccagca cctgggccat ctcgcggttc gagttgccgg cgcgcaccat
     4501 catgccgatg cgcgtcttct ggatgacgaa gtagagcgcc gcggcgagcg ccaggcacac
     4561 gccggagatg aacagccggt agaccgggta gctcagcgtc tcggtgagcg ggatggagta
     4621 atccaggacg gcgggaaccg gcacgccgtg cacatcgttg ccaaacagga tgctgcgcag
     4681 ctcctcgaag atgaggatga ggccgaaggt gagcaggacc tggtacaggt gatcgcgctc
     4741 gtagagtgtg cggatcagcg ccgattccag cagcacgccg atgaccaggg agatcacgac
     4801 gccgatggga agcgcgagcc acaggctgcc ggtttcggcg acgagccagt aggcgaggta
     4861 ggcgccgacc atgtagaagc tgccgtgcgc gaggttgatg acgcccatga tcccgaagat
     4921 gagcgtcagg ccgctcgcga gcaggaacag cagcagcccg tactggatgc cgttgaggat
     4981 ctggatcagg aattccatcc gtcgctcacg caaaagcgcc gaccgaagga agcgcggtcg
     5041 gcgcgaagtg taaaatcgtt acgcgcggtt tgcccggctc ttccccacgg cggggaagag
     5101 cggcggcctg cggcgctatg acatcttgca gccgcgcgcc gggtcggcga gcgccttgtg
     5161 cgccacgccc acgatcttgt tctccttgcc gatgactttg cggagataga agtcctgcac
     5221 cgggttgtgg gccttggaca gggtccaggc gccgcgcggg ctgtcgatct tggccttctc
     5281 catcgccgcg atcagttctt tcttcctgga gacgtcgcct ttcacggcgt tgaggccgac
     5341 gtcgagcagg agcgccgcgt catagccctg cacggcgtaa acgtccggcg gggacttgta
     5401 ggctctcacg taggcgacgc ggaacgcgtt attgcgcggt gtttccagcc cgtcgccgta
     5461 gtgcagggtc gtctccacgc catcggccgc gtctcccgct gcgtccagca cgccgtccgt
     5521 gaggaaaccg gagccataga gcgggatcct cgccttcagg ccggcggccg cgtagtccct
     5581 caggaatttc gcggcgccgc cgccggcgaa gaacgcgacc acggcatcgg gcttggttgc
     5641 cgctgcctcg gtcagcagcg cctggaactc gacctgcggg aagggcagga gcagctcctt
     5701 cacgagcctg ccgccccgct tctcgaaggc ttccttgaag ccgtccagca tctccttgcc
     5761 cgcggcgtag ttccaggtga tgaagaccgc cgtcttgagg cccttgtccg tcagcacctt
     5821 ccccagcggg taggtggtct gccagttaga gaaggaagtg cggaagatat tcggcgcgca
     5881 cgcggccccc gtggcatcgc cggcgccggc gttcgggcag acgagcagcg tgccagtctc
     5941 gcgcacgacc ttgaccatgc ccatgagcac gccggagtgc acggtgccga ccacgacgtc
     6001 caccctatcg cgctgcacca gccggttggt gttctccggc gccttggcgg ggtcggattc
     6061 atcatccacc gtgaagtaat cgatttcgcg gccgccgagc ttgccgccgc gctcggcgac
     6121 ggccagcttg aaggcattgg tgatcgaggt gccgagggcg gcatacgtgc cggtgtaggg
     6181 cagcatcagg ccgatcctga gcttcggttg ctgcgcgaac agtggcccgg ccgggatggc
     6241 gctcccgagc gccagcgtgc cgagtgtttg caggaatcca cggcgataga tggtcatgat
     6301 gtcccctcct ggatagacag ggtaaacgcg tttaccgcct ggcggcaagc tcgcgcagct
     6361 tgaagcgctg gatcttgccg gtggcggtct tcggcaggtc gctgacgaac tcgatccagc
     6421 gcgggtactt gtacggcgcc aggcgcgcct tgacgtgctg cttgagctcc tcggcaagct
     6481 gctccgagcc ctcggccccg ggcttcagca cgacgaaggc gagcggcttg acgagtttct
     6541 ggtcgtcctc cttgccgacc accgccgcct ccagcacgga ctggtgcgtg atcagcgccg
     6601 actcgacctc gaccggtgac acgtacatgc cgctcacctt cagcatgtcg tcggtgcggc
     6661 cagcgtaaac gtagtagccc tgctcgttca ccgtgtactt gtcgccgctg cgcgtccagg
     6721 caccgaggaa ggtggagcgc gagcgctcgc ggttgttcca gtagtagccc gcgctggtcg
     6781 gcccgctcac caggagctcg ccttgctgcc ccggggcgac gtccgccgag ttctcgtcca
     6841 cgacgcgcaa cgcgtagccg ggcacgggct tgccgctggt gccgtagcgc acgtcgccgg
     6901 ggcggttgga caggaagatg tgcagcatct cggtcgagcc gaggccgtcc aggatgtcca
     6961 cgccgaaaca atcgctccag cgcctgccga tatcctgcgg cagggcctcg cctgccgaaa
     7021 cgcagcggcg cagccgcagc tcctcccggc ggggcagctc ggggttggcg agcatcgcgg
     7081 cgtagagcgt cgggacgccg tagaagatcg tcggctggtg cttgcgcagg cgggcgaaca
     7141 ccgcgggcgg ggccgcgcgc tcggccatga cgaccgccgt ggcgccgacc gccagcggaa
     7201 agctcagggc gtttcccagc ccgtacgcga agaacagctt ggcggcggag aacacgacat
     7261 cgctttcctg gatgccgagg accgcccggg catagagctc cgccgtcagg atcatgctcg
     7321 tatggacgtg gacggttcct ttgggcagcc cggtcgagcc ggacgagtag agccagaagc
     7381 aggcgtcgtc ggcggttgtc ggggcggcgt cgaacgcggc ggacgactgc gcgaggaggc
     7441 cggataacga caggtgcccg gcgctgttcc tgccggacac gatcacgtgc ttcagggtgg
     7501 gaaacctgcc gaggaggggg gcaaacacgg gcaacaacgg ctcggacacg accagggcgc
     7561 gcgcccgact gtccttgagc atgtactcgt agtcgccgga ggtgagcagc gtatttgccg
     7621 cgatcggcac gatgccggcc ttgatgctgc cgaggaaggc ggtcgggaag tcgatcgtgt
     7681 cgagcaggca cagcagcacg tgctggtcca tctgcagacc caggccggtc agcgcgtttg
     7741 cccaccggtt cacgcgttcg gcaagttccc cgtagctgta cgagccgccg tcgtcgatgt
     7801 acgcgatatt cccggcgcgg ccggcgcgca ggttacgctc gatcaggtca tgcgccgcgt
     7861 tgtagtcgcg cggatagctg acgaccggcg gatgggcgcg atggtcggcc gagctcagtc
     7921 cgggcattgc ctccccctgt ggcggatcag caatataatg catggacaat cgccacatag
     7981 ctagatgggc atgagccatg gccacgcagc caccggttac ctatgaaacg caccccgatc
     8041 gctacctgca ctggcgcctg tcgttcgatg gccccgtggc gaccctgacc ctggacgtcg
     8101 acgagaaccg cggcttccgg cccggctaca agctcaagct gaactcctac gacctgggcg
     8161 tggacatcga gctccacgac gcgctgcagc ggatccgctt cgagcacccg gaggtgcgca
     8221 gcgtgatcct gaccagcggc aaggaccgca tcttctgcgc cggggccaac atcttcatgc
     8281 tcggccagtc cagtcacgcc tggaaagtca acttctgcaa gttcaccaac gagacgcgca
     8341 acgccatcga ggacgcgagc gcgcattctt ccctcaagtt cctcgccgcg tgcaacggca
     8401 ccacggccgg cgggggctac gagctcgcgc tggcttgcga cgagatcgtg ttgatcgatg
     8461 accgctcctc cgccgtgagc ctgccggagg tcccgctgct cggcgtgctg ccgggcacgg
     8521 gcgggctgac gcgcctgacc gacaagcgca aggtgaggcg cgacctcgcg gatctgttct
     8581 gcacgacggc cgagggcgtg cgcggcgcgc gggcgaagga gtgggggctg gtggacgacg
     8641 tggtgaagcc ccagcagttc gccgggcagg tgaaacaccg ggcgctggaa ctcgccgcgc
     8701 ggagcgaccg gccgcgggac gcccagggcg tcaccttggc gccgctcgag cgccgcgtgg
     8761 acggcgccgg ctaccattac ccgttcatcg acgtgcgcat cgaccgcggc gcgcggcacg
     8821 ccacgatcac ggtgagcgcg ccggatgcgc ctcagccgca ggacctggag ggcatactgg
     8881 cccgcggcgc aggctggtgg ccgctcaggg tggcgcgcga gctcgacgac gccgtgctca
     8941 tgttgcgcac caacgatctc gacatcggca tctggttgct gaagacgcgc ggcagcgcgg
     9001 atcacgcgct ggcggtggac gccagcctgg gccggcaccg ggaccactgg ttcgtgcgcg
     9061 aagttaccgg catgctgcgc cggacgctca accggctcga agtgtcatcg cgcagcctgt
     9121 ttgcactcat cgatgcgggc tcctgcttcg ccggcacgct gctggagctc gcgcttgccg
     9181 cggatcgcag ctacatgcag cagcgtgtgg ccgaaggggc cggcgcgccg gtaatcgcgc
     9241 tctcgccgat gaactttggc gcctaccccg cggttaaccg cgtaaaccgc ctggagtccc
     9301 gctactacgg ggacgccggg caggttgccg cggcgcggcg cgcggtgggg cgcaagctcg
     9361 cggcgggcga ggccctggag ctgggactga tcactgtggc gcccgacgag ctcgactgga
     9421 gcgacgagat ccgcctcgcg ctggaggagc gcgcgagctt gtcgcccgat gcgctgactg
     9481 ccatggaggc gaacctgcgt tgcggcggcg ccgagaccac ggagacgcgc atcttcggcc
     9541 ggctgtctgc ctggcagaac tgggtattca gcaggcccaa cgccgtgggg gacgcgggcg
     9601 cgctcaaggt attcggcacc ggtaacaagc cccgattcaa ctgggaacgg gtgtaggcgg
     9661 gctgtcgccg ggaagcgctc cacagcatcc gatgacgatc gactatagcg cgaagatacc
     9721 caacaacgtc gacctttcgg gcgacaaggc gctgcagcgc gcgctcgagc gctggcaacc
     9781 cgcgttcgtc ggctggtggg gcgagatggg gcccgaggcc gctcgcggtt gcgacgtcta
     9841 cctgcgcacg gccatcggtg tcgaccggga cggctgggcg aatttcggct acgtgaggat
     9901 gcccgactat cgctggggca tcttcctcaa cccgggcgag cccggccgca cgatcggctt
     9961 cggggaccac aagggcgaac cggcctggca ggaggtgccg ggcgaatacc gctccaacct
    10021 gcggcgcatc atcgtcacgc aaggcgatac cgagccggcc tcggtcgagc aacagcggcg
    10081 gctggggttc tcgtgcccct cgctctacga cctgcgcaac ctgttccaga tcaacgtcga
    10141 ggagggccgc cacctgtggg cgatggtgta cctgctgcac cgctacttcg gccgcgacgg
    10201 ccgggaggaa gcggaagcgc tgctcgagcg ccgctccggc gaccgcgaca acccgcgcat
    10261 cctcggcgcg ttcaacgagg ccacgccgga ctggctgtcg tttttcatgt tcacgttctt
    10321 taccgaccgc gacggcaagt tccagctcac cgccctcgcc gagtcgggct tcgacccgct
    10381 ggcgcgcacc acgcggttca tgctgaccga ggaggcgcat cacatgttcg tcggggtgac
    10441 gggcatcacg cgcatcctgc agcgcacggc gcaggtgatg gcgcagcaca agaccgacga
    10501 cccggcgcgg ctgcgcgccc tgggcgtgat cgacctgccg accatccagc gctacctgaa
    10561 tttccattac agcatcacgc tcgacctgtt cggctccgat gtttcgtcca acgccgccac
    10621 gttctacacg acggggctca agggacgctt cgacgaggcg aggctggacg acgaccaccg
    10681 gctcaaggac ctgacctacc cggtccccga agtggccggc ggccggatcg tgcagaagga
    10741 agcgcccgcg ctgaatgcgg tgaacgagag gctgcgcgac gactacatcc aggacaccct
    10801 ggccggggtc gcccgctgga atcgcgtcgt gcagaaaggc ggcgtaggct tccagctcca
    10861 ggtcccgaac aaggggttca accggaggat cgggtccttc gcgggcctga gaatttcacc
    10921 tgacggtcgc gtggtgagcg agaaggaatg ggaagcgaac gttgcggcgt ggctgccgac
    10981 cgcggaagac cgcgccttcg tgcaatcgct catggggcgc gtggtcgagc cgggcaagtt
    11041 cgcgaactgg atcgcgccgc cggagcgcgg catcgacggg cagccggtca acttcgaata
    11101 cgtgagattc aactgatcgc gaccggcgac ggcgcttcgg gcagcgtcag gccaagcttc
    11161 gccagccggg cttcgacatc gatgctcatg gttgctagac gtcctcgtcc ggggagcgga
    11221 agcgcgccgc gctgaagtcc acggccagcg ccgccagcgc gagcaccagg atcgcgccgg
    11281 ttgcggttag gatctgctcg ttgttcaggt ccttgatgtc gatggtcagc atgcgggtga
    11341 gcgccgtgat cgcgacgtag atcaggaagc gcaccggcag gcggtgggtc ttgaaataga
    11401 tgccgaccat cgcgccgatc tccaggtaga tgaacagcag caggatgtcg tggagcttgg
    11461 ctgcgccccg ctgcatgatc tcgacaaggt agaagtgcgc ggcggaccac accaccgtgc
    11521 cgccgatcac gaacagcgca agcagatgga agcccttgac caggacacca ccgaaggcct
    11581 cgatcctctc gatcatggtt cctcccgttt catgtcatga cgcgcgcggc gcccccaccg
    11641 cgccatgtgc ttgggttcgc gcggctgcct tgcgccacag cgcgggcaga acacgtcctt
    11701 cttgccgagc ggctgaccgc attcgaagca tctgcggagc agattcatgg aatccgtgag
    11761 atcgtgagat cgggaaatcg ggaggtcgaa ttgccgggag atcgtaaggt cggataccca
    11821 aagttctgct ttgctctccc gcactcacgt tctcccgctc tcacggattt tatcgctctc
    11881 acgatctccc gccctcacgc tctcccgtct tttctcgtgg gcgccgtcag gcgcgcacga
    11941 gcttctcctc gaaccagttc cacatctccg ccaccaccag cgtgaggtag tcacgctggc
    12001 agtgctgcgc gccgccttcc gcggcggtga acacgcggaa ggtcttgtcc ctcgagccgc
    12061 aggcgtcgaa ctgcttctgc gcgtcggtga gcgggatctg ctcgtcgtcc gcgccgtgca
    12121 ccagcaggaa cgggcagcgc atcttctgca tcacgccgtc gagcctgaac ggctcgagct
    12181 tcttcaacgc ctcgtcgagc gatgccgcgc cgagaatcca ctcgatgtgg tgccccggca
    12241 ccgagagcgc ggtcttgaag gaggccgcga cgcgcttctt ccaggtgtcg tgatagtccc
    12301 actgcgcgcc ccaggcgatg caggcggcga aacgcggctc catcgctgcg cagcgcggcg
    12361 cgtagtagcc gccgaggctg atcgcgacga tgccgatttt cctcgggtcc acatcggcgc
    12421 gcttttcaag ccagttgatg cacgcggaac ccgcgacctc gtagtcgtgg cgca
//
LOCUS       DBNGWZ010000412        17886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_177432, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000412 DBNGWZ010000000
VERSION     DBNGWZ010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_177432"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16CX_12300"
     CDS             <1..474
                     /locus_tag="AC16CX_12300"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3013327.1"
                     /translation="EHAPRNGLVVASLDRQAVMELYAMREVLEGTAARLCARHASDME
                     VLDLLDLVEQERQMQGDFDALARHNLRFHEAIRRGAHNRYLVKSLSAVNDSMVLLGRP
                     LMLLPHRAQAALAEHESIAQAIQRRDPEAAEEVARQHVRAAQRERLKRLFPDSAA"
     gene            508..1680
                     /locus_tag="AC16CX_12305"
     CDS             508..1680
                     /locus_tag="AC16CX_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3013328.1"
                     /translation="MAIDRIEVRVTDLTVRLQRETSSGAYDTGPAGTLLGKPVLVKIH
                     ADGVTGYGQIRPISPGHFMPDTVHSMVGAITDVYGPRLIGRDIHDWASMHDMFEQALP
                     ANMNARAALDHALHDAAGKALNLPVYKLLGGLCQESIPLEWSVSMASDPGKMVEDAER
                     AMRQYGIRVLCLKAGGKGGWQRDVKNFITVRKAMGEEPVIGVDPNEGWTVSETIRAVG
                     ELAPHRLDYLEQPVKRQNIAGMAAVRRALSGVPLMADEGVMTLGDAYALAKAEAVDVF
                     CVKLYKMGGLRPAKKIAAVADAAGIQLNIGGLAVLSQLEAAAGAHFYASSPARSVMPA
                     AEFIFGLGVLGPDPLVPETDFVIRNGHVTPPGRPGLGIAVDERALERYTLKREIVK"
     gene            1733..3013
                     /locus_tag="AC16CX_12310"
     CDS             1733..3013
                     /locus_tag="AC16CX_12310"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HIR3013329.1"
                     /translation="MGQTMAEKLLTRHNLAGKAARAGDIVEARIDGAMCHYHAADPMH
                     DMAVQSGFEGGLPRVWDPERIFVLVDHHQPALSQKLADENAVIRREVARLGIQCFHDA
                     EPGIAHQMMLDYGLVRPGELVVGNDSHTLSYGAVNAGATGIARADMFYVLLFGELWFQ
                     VPSSIKVVLDGRQPGYPIAKDIILYLAGRYGDDFAGNLSIEYSGSLVPQLSIDSRMCL
                     SAHGVEVGAKFAMFPEDDKTRAFLKGRTDQRYEPLAPDPGAEYEKEIRLDVDAMPFVV
                     AKPHQFGNVAPVDEVKGTRIHQAQIGSCANGRFEDIEIAARMLAGRKVAKGVRFIISP
                     ASQQVYLQCLKAGLIEKLVEAGAQVVTPGCGVCQPRVGFLSDGEVCITATTRNFKGRK
                     GSLNAEIYLAGPLTVTAAAVAGEIVNPKEVFRGL"
     gene            3003..3530
                     /locus_tag="AC16CX_12315"
     CDS             3003..3530
                     /locus_tag="AC16CX_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR3013330.1"
                     /translation="MGFDLNRPIRGRIWKFGDSIETDAINPYYRYPTMEELKQHTMEA
                     YRPEFPREVRPGDLIVAGRNFGCGSSRPGLVLREVGVAAIVAETVSRLFLRNSIARAV
                     PIFMAPGVTGIVQDGETLEVDYPQGVVRNPATGAAVALRKFPPLIERIFQCGGLPEFA
                     RERYLKEAALASRNG"
     gene            3619..5124
                     /gene="tcuA"
                     /locus_tag="AC16CX_12320"
     CDS             3619..5124
                     /gene="tcuA"
                     /locus_tag="AC16CX_12320"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3013331.1"
                     /translation="MENASYDVIVVGGGNAALCAALSAREEGASVLVLERAPVEKRGG
                     NSAFTGGGFRFVHHGAEDIRKAVPDLTDEEIARTDFGQYTAEDYLDDLGRVTQYYIDP
                     DLAETIVRNSADTVQWLRGRGVRFVPNYGRQAFNVNGRFKFFGGVVIHANSGGRGLVE
                     YLYKAVEKHGIAVRYGAQAKDLLRGPGGIEGVRVILDGKEEVIRARSVVLACGGFEAN
                     REWRTRYLGPGWDMAKVRGTRYNTGDGIRMALEIGAQSYGQWSGCHSVAWERYAPDFG
                     DLESRSSSYRHSYPFGIMVNAEGRRFVDEGADFRNYTYAKYGRIVLQQPGGYAWQIFD
                     AQVAHLLREEYKLRGATKAEADTLEALVDRMHDVHPARFLETVREYNAAVKRDVPFDP
                     NVKDGRCTTGLAINKSNWANTLEVPPFQAYSVGCGITFTFGGLKINPAAHVLDMEDQP
                     IPGLYAAGELVGGLYYFNYPGSAGLMGGAVFGRIAGRESARHARGAQSAAA"
     gene            5127..6062
                     /locus_tag="AC16CX_12325"
     CDS             5127..6062
                     /locus_tag="AC16CX_12325"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3013332.1"
                     /translation="MNPGKKTSRLRRLIHRRDKVLVVLHPPTATHARIMEQAGCEAGF
                     VGTSGVVGSYTGLADVGTATMTECVQIAGWIAQSVNFPVIMDGDTGHGGIMAVRRMVR
                     ECIRAGIAGVRIDDQPIEGKRRTQSAGIEVVPLEQAIARYRAAVDMKNELDPDFVVMA
                     QCYARDAANGGLEECIGRLRAYKNEAGVDWVQLESPHSVEEIKQARAAVEGPFSFMKG
                     KLPRYLGLDEHLALGVNIAWYPGFTHQVMWAALWDFMQDFQKRGIAAWEDFVESRKDR
                     PFPRPDVGPEGEGGEKQRALEERYFSSAALEKYRK"
     gene            6101..7213
                     /locus_tag="AC16CX_12330"
     CDS             6101..7213
                     /locus_tag="AC16CX_12330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3013333.1"
                     /translation="MKDEVVLLGCGDVGPIHEPVDGYATLAKPTLAGADIRFAQVERV
                     YSEGGALQVHSGGAHSRVKPHMASVFSDCGFDVVSLASNHAMDWGEDALLDTIELFQA
                     RGMQVIGAGRNLHEARRPAILERNGVRIAALAYCSILQTGYAAGPDKAGIAPLRAHTY
                     YEPFDYQPGVPPRVVTVQYEEDLSGMMEDIAEARKTAHVVVLSLHWGIHFIPRVIADY
                     QVAVAKAAFEAGADLILGHHAHAPKAIGVYGGKACFFSLSNFIMSSTAKTPAKAAEFM
                     KRYGVVMDSDYPHLAYGTDAKRSLIAKAVLTREGVKRVSFLPVLIDKQLRPEVLRRGD
                     PRFDDAVRFMEWVSEGFEHQFSVEGDEVRVTGDGGA"
     gene            7233..8228
                     /locus_tag="AC16CX_12335"
     CDS             7233..8228
                     /locus_tag="AC16CX_12335"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013334.1"
                     /translation="MNAMTGIRKLGVLVLLACAASPALAQQKAARLPDGFPSKPIRII
                     VSSAPGGGQDLTTRPVAQKLGEDLGVSVVVDNRGGGSGVIAMDITRQAAPDGYTLLVG
                     ATTMILTSVTKRVPYDVRTAYEPVVQMTTQPYFLVVHPSVPVKTPEEFVAYAKGRPGA
                     LSYGTSGQGSLHFYGMELFNSIVGIKTVHVPYKGSGPALIDLLSGQIQLMFTSTVSAI
                     PHLRSGKLKAVAFTGRNRTQIFPQAPTLSETVAPGFELKNMYCLFAPAKTPKPVLALL
                     NREVAAIVNAGEVRNKLAADGAEPAPPVSVDEFRSTYHREVGTWEKLVKNLKAEM"
     gene            8265..9254
                     /locus_tag="AC16CX_12340"
     CDS             8265..9254
                     /locus_tag="AC16CX_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013335.1"
                     /translation="MKTVRERAYAVFLALVISLAPAASLAQQKDTRGGYPRKPVRVLV
                     GSPAGSGSDLMMRAVAQKLTERWGQSVIVDNRAGAAGAISLQLATQAVPDGYTLATLS
                     AQNVTAMILGTVTTDIPKELTPVVLMITQPYLMVATPSLPANSVKELIALAKAKPLVY
                     ASSGIGSVVHLGMELFKSMAGVEMTHIPYKGSGLSMIDVMSGRVQLAITNTLTATPLV
                     RGGKLKALAITSPQRVQAMPELPTVSEAGVPGYELRSWYGLLVPNKTPPSLALMLNRE
                     VSETMNAPEVRDRLAADGAEAAAPNTPAQFRGLIAGEIAKWEKLIKALGMQAK"
     gene            9268..10263
                     /locus_tag="AC16CX_12345"
     CDS             9268..10263
                     /locus_tag="AC16CX_12345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013336.1"
                     /translation="MKTGTPISKAALCVLLAGVATAAHGERPSGLPGGYPAKAVRVMV
                     GTSPGGGVDTITRGVAQKLSEQWGQSFVVENRTGGGGVLAVTLLAQAAPDGYTLYGGG
                     SQVVTATPLKKVPFDTRVVLQPVVQMTSSWYFLLVHPSLPVRSVKELVALAKKRPGTL
                     NYGSAGTGSASHLGGELFKFKTGIEVMHIPYKGNGQALIDLVSGQTQMMFTSTISGSP
                     HVRSGKARGIAVTASKRLAAYPDVPTVSESGVPGFELDNIYGIYAPTGLAPAILGALN
                     REIGQIIASPEMRGRITADGAEPAPPHSAAEFRDRFAREIGTWEKFIWTSGIKVE"
     gene            10286..11734
                     /locus_tag="AC16CX_12350"
     CDS             10286..11734
                     /locus_tag="AC16CX_12350"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013337.1"
                     /translation="MNPETAMNPLADSDAGRDATRKLARHAATLAYESLPRALVELTK
                     QCVLDTLGVTIGASSLAPEAKIVAEYVSDLDGKPEATVLGFGGKAPAPWVVFANGSLG
                     HMLDYDDLGGGHVSIVTVPVALALAEKAGGMSGRDLITAIAAGADVMTRLTLAIRIPD
                     WTMTEGWFATQLLGFIGGAATAGRLLGLDEEQMENALGIGFNQMSGSRQMAVGAATHM
                     RSMQAGFSGQGAVLAAELARRGIIGSKEILEGRYGLFKTYVRTNEPDWNAILGGLGAR
                     FPLLDTHGFKVWPACGYTRPVNAALLQLREELGLRPDDVESISVVGGTRATQLLSEPL
                     ERKRRPETSIDGKYSIPFTTAVMMVKGNVTLLDYTDEGLRDPAVLAMADRVSYRAGGA
                     FPDVIGGSSSVSRPTVEIRTRDGRMHARKAEHLPGDPKQPVKREFLEAKFRDCARFSA
                     KPVATGNVDRAVEMIWDLENVADATEVVRLLA"
     gene            11767..12924
                     /locus_tag="AC16CX_12355"
     CDS             11767..12924
                     /locus_tag="AC16CX_12355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3013338.1"
                     /translation="MAQGSSEVTFIGTGDCGPVHGPREGFPIERYTELVRPVLEKVDL
                     RFGNCERQYSSRGTASERAPHGRQPPEMAKIFTDCGFDAVTIANNHMYDYGPDALLDT
                     RVLLLEKGIKVTGAGKNLDEAREPAIVEKNGVKVGFLGYCSVIPEGGEAGPGKVGIAP
                     LRVRTYYEPRGPHAPVRVLTEPDEQDIRMILDDIAALRKRVDIVIPVFHWGVIWVPRV
                     IADYQVTVAHACIDAGADMVLGHHAHVPKAIEMYRGRAIFYSLSNFCMTKPFPSPKWK
                     EEPWKHGALRNHADQDPDMPLLPYGRDAKRSLLAKAVFTKSGVRRVSFLPMMIDKLYR
                     PEVLRGGDPRFEDMVRYMEWASEGFDHHFEIAGDEVIVTAQPAAPARKEAA"
     gene            12921..14318
                     /locus_tag="AC16CX_12360"
     CDS             12921..14318
                     /locus_tag="AC16CX_12360"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013339.1"
                     /translation="MSDAGLKSDGLQQRLADYARALTYDALTPAAAHAAKVRVIDTLA
                     ALIGGFDGEPCRIARAMAARAPLADGATVYGTATRTTPEMAAFVNATAARYVELNDVY
                     HWPGSAGGHPSDVIMPLLGVAEYARATGRDLITAVVLAYEIYLRLSDAIGRTAFDCAN
                     FACLGVAAGSARLLGLAPEQLAHSLSMAVVPNNILRQVRTGHLSMWKAVTAGHAGKAG
                     VFAALLAREGMNGPFLPFEGRAGWCDHIAGRRFSLDAMGGNGAPFKIQDTMIKPRASC
                     ATTISSILAAEKVAPAVRGRIAEVERVTVETYERAKAGMGTGEHHWNPDSRETADHSI
                     PYVVAAALMDGTVTPRQFDEAHLHDPGLHALLGKIDVVANEEFTRAYERLPVEHRTRV
                     NVVMRGGERFTGEAGGDKGDLSEHKSDAQIAEKFLGVTEAFLGARRARATLARLWELD
                     GLDDVARLPEGFVQG"
     gene            14334..15485
                     /locus_tag="AC16CX_12365"
     CDS             14334..15485
                     /locus_tag="AC16CX_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013340.1"
                     /translation="MAKNGFKIFDSDTHVGPYVDVLEKYLTEAEKSKLPAWEQYKSST
                     SRGYAIYARGQRHYRRRLGAADPQDTRGKYMAGFTGAHGARKPSPRVDYDPAERIADM
                     DYEGVDVNLMLPSGWFGTWTASDDVALEMGMYRAYHRWMEDYCSAYPERLGGVILACA
                     RDIEGSLAEIRRWGKSRWAWGLMVYAPHGMPLDHPDLEPVWAAAQDHDLSIALHTFTV
                     MPPYAPGGLDTWDNLFLQRSAAHPWCGMRNMAALIGAGVMDRYPRLRIGTLEAGHGWL
                     PFWMERLNEHARTIRSALPRLKQTPGEYVTGGRYFQSIEAPEGAKLTNAVIDLIGEEV
                     LMYASDYPHGESHFPHTVEMVMGWEMPEARKRKLFWDNAIRYYARCGLK"
     gene            15526..16506
                     /locus_tag="AC16CX_12370"
     CDS             15526..16506
                     /locus_tag="AC16CX_12370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013341.1"
                     /translation="MKHRRVTAVLAAAAALSPCAVSAQSPADSYPVKPVRVIVGLAPG
                     GGTDIQTRLLAQKLSSNLGRSFVVENRTGAGGTVAYALVAKSPADGYTLLGVASGYSI
                     TPAVYPKLAYDPIKDFAPISLVVQAPLLLMTHPSLPVRSTRELVALAKRKPGMLDSAS
                     AGHGTSNHMALELFNLLAGVKITHVPYKGTGPALIDAMAGQVHMIFGNILSSLQYVRQ
                     GRLKALAVTSATRSKAVPELPTIAESGVPGYETTTWHGWLAPAGTSAAIVNRMSVELA
                     RAVKSPDVQGHLAGDGGEAIGSGPEQFAQHIATEIARWRKVVQASGMRVE"
     gene            16523..17512
                     /locus_tag="AC16CX_12375"
     CDS             16523..17512
                     /locus_tag="AC16CX_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013342.1"
                     /translation="MTRQHSFAAAWIVAAAVGLPGAVSAQRTDGFPTKPVRVVVPQAP
                     GGATDIQARLFAVRMGQNLGQQFIVDNRAGGGAAGVVAFSTVARATPDGHTLLAIIPS
                     FTFSPALYKNYPVDPVKDFAPISLLTRAPYLLVVNASMPAKSVKEFIAHARAQPEGPL
                     FGAGNTGSGTHLVTMWFLTAANVRASYVPYRSVGLAMLDLIGGRIDATLANVLSVGPY
                     VRAGKLRALGISTARRSRVYPDLPTIAEQGVPGYDASTFHGYAAPAGTPAAVVNKLSA
                     EFAKVVKSSDVAERISEDGGEPIGGTPKEFRQFIAAEILVWHKVVKDAGISVQ"
     gene            17588..>17886
                     /locus_tag="AC16CX_12380"
     CDS             17588..>17886
                     /locus_tag="AC16CX_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3013343.1"
                     /translation="MLKAAIYGMGRWGTRLVESVQPSEKIRIVKGISRDPEKHREFSE
                     KTGIRFISSYGRVLKDPEIGAVVLATPHSLHWKQIVQAAKAGKHVFVEKPLTLTR"
BASE COUNT         3154 a         5810 c         6162 g         2760 t
ORIGIN      
        1 gaacacgcgc cgcgcaacgg gctggtggtc gcgagtctcg accgccaggc ggtcatggag
       61 ctctacgcca tgcgcgaggt gctggagggc acggcggcgc gcttgtgcgc gcggcacgcc
      121 tccgacatgg aagtgctcga tctgctcgat ctggtcgagc aggagcggca aatgcagggc
      181 gacttcgatg ccctggcgcg ccacaacctg cgtttccacg aggccattcg tcgcggcgcg
      241 cacaaccgct acctggtgaa gagcctctct gcggtgaacg actcgatggt gctgctcggc
      301 aggccgctga tgctgctgcc ccaccgcgcg caggcggcgc tcgccgagca cgagagcatc
      361 gcgcaggcca tccaacggcg cgatcctgag gcggccgagg aggtcgcgcg ccaacacgtg
      421 cgcgccgcgc agcgcgagcg gctgaagcgc ctgttccccg attccgcggc ctgatccggt
      481 ccacgccgcg acgagaggag gccggtcttg gccattgacc gcatcgaagt ccgggttacc
      541 gacctgaccg tccggctgca gcgcgagacc tcgagcgggg cgtacgacac cgggcccgcc
      601 ggcacgctgc tcggaaaacc cgtgctggtg aaaatccacg ccgacggcgt cacgggctac
      661 gggcagatcc ggccgatcag tcccggccat ttcatgcctg acaccgtgca cagcatggtc
      721 ggcgcgatca ccgacgtcta tgggccgcgt ctcatcggcc gggacatcca cgactgggcg
      781 tccatgcacg acatgttcga gcaggcgttg cccgccaaca tgaacgcgcg cgccgcgctc
      841 gaccacgccc tgcacgacgc ggccggcaag gcgctgaatc tccctgtgta caagctgctg
      901 ggcggcctgt gccaggaaag catcccgctc gagtggtcgg tcagcatggc gtctgatccc
      961 ggcaagatgg tggaggacgc cgagcgcgcg atgaggcaat acggcatccg cgtgctgtgc
     1021 ctgaaagccg gcggcaaggg cggatggcag cgcgacgtga agaatttcat caccgtgcgc
     1081 aaggcaatgg gtgaggagcc tgtcatcggc gtggacccga acgagggctg gaccgtgtcg
     1141 gagacgatcc gcgccgtcgg cgagctggca ccgcaccgcc tcgactacct cgagcagccg
     1201 gtgaagcgcc agaacatcgc cggtatggcc gcggtccgcc gcgcgctttc cggcgtgccg
     1261 ctcatggccg atgaaggcgt catgacgctg ggggacgcgt acgcgctcgc gaaagcggag
     1321 gcggtcgacg ttttctgcgt gaagctctac aagatgggcg gcctgcgtcc cgccaagaaa
     1381 atcgccgccg tcgccgacgc agccggcatc cagctcaata tcggcgggct cgcggtgctc
     1441 tcgcagctcg aggccgcggc cggggcccac ttttacgcga gttcgcccgc gcgcagcgtg
     1501 atgcccgccg cggaattcat cttcggactc ggcgtactgg gtcccgatcc gctggtgccc
     1561 gaaaccgact tcgtgatcag gaacggtcac gtcacgccgc cgggccgtcc gggcctgggg
     1621 atcgccgtgg acgagcgcgc gctggagcgt tacacgctca agcgggagat cgtcaaatga
     1681 gatccactgc ggcggcacgc ggcgacgagt gagtcaattc gagggagcac gtatgggaca
     1741 gaccatggcg gaaaagctgc tcacacggca caacctggcg ggaaaagcgg cgcgcgcggg
     1801 agacatcgtc gaggccagaa tcgacggcgc gatgtgccat taccacgccg ccgatccgat
     1861 gcatgacatg gcggtgcagt ccggattcga ggggggattg ccgcgggtgt gggacccgga
     1921 gcgcatcttc gtcctggtgg accaccatca gccggcgctc tcgcagaagc tcgccgacga
     1981 aaacgccgtg atccgccgcg aggtggcgcg gctcggcatc cagtgcttcc acgacgccga
     2041 gcccggcatc gcccaccaga tgatgctcga ctacggcctg gtgcgcccgg gcgagctggt
     2101 ggtgggcaat gactcgcaca cgttgagcta cggggcggtc aacgcgggcg cgaccggcat
     2161 cgcccgcgcg gacatgttct acgtgctgct gttcggcgag ctgtggttcc aggtgccgtc
     2221 ctcgatcaag gtcgttctcg acggccgcca gcccggctat cccatcgcca aggacatcat
     2281 cctttatctc gccggccgct acggggacga tttcgccggc aacctctcga tcgagtactc
     2341 gggatcgctc gtgccccagc tctccataga cagccgcatg tgcctgtccg cgcacggcgt
     2401 ggaagtggga gccaagttcg cgatgttccc ggaggacgac aagacccgcg cgttcctcaa
     2461 ggggcgcacg gaccagcgct acgagccgct cgcgcccgat cccggcgccg aatacgagaa
     2521 ggagatccgc ctcgacgtgg acgcgatgcc gttcgtggtc gcaaagcccc accagttcgg
     2581 caacgtcgca cccgtggacg aggtcaaggg aacgcggatc caccaggcgc agatcggatc
     2641 ctgcgccaac ggccgcttcg aggacatcga gatcgcggcg cggatgctcg cggggcgcaa
     2701 ggtggcgaaa ggcgtgcgct tcatcatttc gccggcctcc cagcaggtct acctgcagtg
     2761 cctcaaggcc gggctgatcg agaagctggt cgaggcgggc gcccaggtcg tcacgccggg
     2821 ttgtggggtc tgccagccgc gcgttgggtt cctctccgac ggcgaggtct gcatcacggc
     2881 gaccacgcgc aacttcaagg gccgcaaggg cagcctcaat gcggaaatct atttggcggg
     2941 tccgctgacg gtgacggccg ccgcggtggc cggcgaaatc gtgaacccga aggaggtgtt
     3001 ccgtgggctt tgatctcaac cgcccgatcc gcgggcgcat ctggaagttc ggcgactcga
     3061 tcgagaccga tgcgatcaat ccctactacc gctatcccac catggaggag ctcaagcagc
     3121 acaccatgga ggcgtaccgg ccggagtttc cgagggaggt cagaccggga gacctcatcg
     3181 tcgccggccg caacttcggc tgcggctcga gccggccggg gctggtgctg cgcgaggtcg
     3241 gcgtggccgc gatcgtcgcc gagaccgtct cgcggctgtt cctcaggaac agcatcgcgc
     3301 gcgcggtacc gatcttcatg gcgcccggcg tgaccggcat cgttcaggac ggcgagacgc
     3361 tggaagtgga ttacccgcag ggcgtggtcc gcaacccggc gaccggcgcg gccgtggccc
     3421 tgcgcaagtt cccgccgctg atcgagcgta tcttccagtg cggcgggctg cccgaattcg
     3481 cgcgcgagcg ctacttgaag gaagccgcgc tcgcaagccg caatggctga ccggccggcg
     3541 agcgcggatc acccgaaaac accaacccgg caagcctgcg gcccggcatc cggcgcaggc
     3601 gggcaaacag gagaacacat ggaaaacgca tcgtatgacg tgatcgtcgt cggcggcgga
     3661 aacgccgcat tgtgcgcggc gctgtccgcc cgcgaggaag gagcgagtgt gctggtgctc
     3721 gagcgcgcgc cggtggaaaa gcgcggcggc aacagcgcgt ttaccggtgg cggcttccgt
     3781 ttcgtgcatc acggcgccga ggacatcagg aaagccgttc ccgatctcac cgatgaagag
     3841 atcgcgcgca ccgatttcgg ccagtacacc gcggaagact acctggacga cctcggccgc
     3901 gtcacgcagt actacatcga tcccgacctc gccgagacca tcgtgcgcaa cagtgccgat
     3961 accgtgcagt ggctgcgggg gcggggcgta cgcttcgtgc ccaactacgg gcgccaggcg
     4021 ttcaacgtga acggccgctt caagttcttc ggtggcgtcg tgatccatgc caacagcggc
     4081 gggcgcgggc tggtcgagta cctctacaag gcggtggaga agcacggcat cgcggtgcgc
     4141 tacggggcgc aggcgaagga cctgctgcgc ggtccgggcg gcatcgaagg cgtgcgggtc
     4201 atcttggacg gcaaggagga agtcatccgc gcgcgctcgg tcgtgctcgc ctgcggcggc
     4261 ttcgaagcga accgcgagtg gcgcacccgt tatctcgggc ccggctggga catggcgaaa
     4321 gtccgcggca cccgctacaa caccggcgac ggcatccgca tggcgctcga gatcggcgcg
     4381 cagtcgtacg gccagtggtc gggctgccac tcggttgcct gggagcgcta cgcgccggat
     4441 ttcggcgacc tcgagtcgcg ctcgagcagc taccggcaca gctatccctt cggcatcatg
     4501 gtcaacgcgg agggcaggcg cttcgtggat gaaggcgcgg acttccgcaa ctatacctac
     4561 gccaagtacg gacgcatcgt gctgcagcag ccggggggct acgcgtggca aatattcgac
     4621 gcgcaggtcg cgcacctgtt gcgcgaggag tacaagctgc gcggggcaac caaggcggaa
     4681 gccgacacgc tggaagcgct ggtcgaccgc atgcacgacg tgcatccggc gcggttcctc
     4741 gaaaccgtgc gcgagtacaa cgccgcggtg aagcgcgacg tgccgttcga ccccaacgtg
     4801 aaagacggcc gctgcaccac ggggctggcg atcaacaagt ccaactgggc caacaccctg
     4861 gaagtgccgc cgttccaggc ctactcggtc ggctgtggca tcaccttcac attcgggggc
     4921 ctcaagatca acccggcggc gcacgtgctc gacatggaag accagcccat ccccgggctc
     4981 tacgcggcgg gcgagctggt cggcggtctc tactatttca actatccggg cagcgccggt
     5041 ctcatgggcg gcgcggtgtt cgggcgcatc gccgggcgcg agagcgcgcg ccacgcgcgc
     5101 ggcgcgcaat cggcggccgc ttgagcatga atcccggcaa gaagaccagc cgcctgcggc
     5161 gcctcatcca ccgcagggac aaggtgctcg tcgtgctgca cccgccgacg gcgacgcacg
     5221 cgcgcatcat ggagcaggcg ggctgcgagg ccgggttcgt cggcacgagc ggcgtcgtcg
     5281 gctcctacac cggtctcgcc gacgtcggca cggcgaccat gaccgagtgc gtgcagatcg
     5341 ccggctggat cgcgcaaagc gtcaattttc ccgtgatcat ggacggcgac accgggcacg
     5401 gcggcatcat ggcggtgcgg cgcatggtgc gcgagtgcat ccgtgcgggg atcgccggcg
     5461 tgcgtatcga cgaccagccg atcgagggca agcgcaggac gcagagcgcc gggatcgaag
     5521 tggtaccgct cgagcaggcg atcgcgcgct accgcgccgc cgtggacatg aagaacgagc
     5581 tcgacccgga tttcgtcgtc atggcccagt gctatgcgcg cgacgccgcg aacggcgggc
     5641 tcgaggagtg catcgggcgt cttcgggcgt acaagaacga ggcgggcgtg gactgggtgc
     5701 agctcgaatc gccccattcc gtggaagaaa tcaagcaggc gcgcgcggcg gtggagggcc
     5761 cgttctcctt catgaaggga aagctgccgc gctatctggg gctcgacgag catctcgcgc
     5821 tgggcgtcaa catcgcgtgg tatcccggct tcacccacca ggtgatgtgg gcggcgctgt
     5881 gggacttcat gcaggatttc cagaagcgcg gcatcgcggc gtgggaggac ttcgtcgagt
     5941 cccgcaagga ccggccgttc ccgcgtcccg acgtcggccc cgagggcgag gggggcgaga
     6001 agcagcgcgc gctagaggag cggtattttt cgagcgctgc gctcgaaaaa taccggaaat
     6061 gacaagtgac gacaaacgag tgacgagaaa cgagtaacgg atgaaagatg aagtcgtcct
     6121 cctcggttgc ggcgacgtcg gcccgataca cgagccggtg gatggctatg caacgctcgc
     6181 gaagccgacg ctagccggag ctgacatccg cttcgctcag gtcgagcgcg tctactccga
     6241 gggcggcgcg ttgcaggtcc actcgggcgg cgcccacagc cgcgtcaagc cgcacatggc
     6301 ttcggttttc agcgactgcg gtttcgacgt ggtctcgctc gccagcaacc acgcgatgga
     6361 ctggggggag gacgccctgc tcgacaccat cgagctcttc caggcgcggg ggatgcaggt
     6421 gatcggggcg ggccgcaacc ttcacgaagc gcgtcgtccg gcgatcctgg aacgcaacgg
     6481 cgtgcgcatc gccgctctcg cgtattgctc gatcctgcaa acgggctatg cggccggtcc
     6541 cgacaaggcc gggatcgcgc cgctgcgggc gcacacctac tacgagccgt tcgattatca
     6601 accgggcgtg ccgccgcggg tcgtcaccgt tcagtacgag gaagacctct ccgggatgat
     6661 ggaggacatc gccgaggcga gaaaaaccgc ccatgtcgtg gtgctgtcgc tccactgggg
     6721 catccatttc attccccgcg tgatcgccga ctaccaggtt gcggtggcga aggcggcatt
     6781 cgaggcgggc gcggatctca tactcggcca tcacgcgcac gcgcccaagg cgatcggggt
     6841 ctacggcggg aaagcctgct ttttcagctt gagcaacttc atcatgtcct cgaccgccaa
     6901 gacgcccgcg aaggcggccg aattcatgaa gcgctacggg gtcgtcatgg actccgacta
     6961 cccgcatctc gcgtacggca cagacgccaa gcgcagcctg atcgccaagg cggtgctcac
     7021 gcgcgagggc gtgaagcgcg tctcgttcct gccggtgctg atcgacaaac agctgcggcc
     7081 cgaggtgctg cgccggggcg atccgcgttt cgacgacgcg gtgcgcttca tggagtgggt
     7141 gtcggaaggt ttcgagcacc agttctcggt cgagggcgac gaagtccggg taaccggcga
     7201 cggcggcgcg tagccaggac acaggagagg tcatgaacgc aatgactgga atccggaagc
     7261 tgggtgtgct ggttctcctt gcgtgcgcgg cgagcccggc actcgcccag cagaaagctg
     7321 cccggctgcc ggacggcttc ccgagcaagc ccatccgcat catcgtcagc tccgcgcccg
     7381 gcggcggcca ggacctgacg acgcgcccgg tggcgcagaa gctgggcgag gatctcggtg
     7441 tatccgtcgt cgtcgacaac cgcggcgggg gctcgggcgt catcgcgatg gacattacgc
     7501 ggcaagcggc accagacggc tacacgctgc tcgttggcgc aacgaccatg atcctgacga
     7561 gcgtcacgaa gcgagtgccg tacgacgtgc gcaccgctta cgagccggtc gtgcagatga
     7621 ccacgcaacc ctatttcctg gtggttcacc cttcggttcc ggtcaagacg ccggaggagt
     7681 ttgtcgccta tgccaagggc cgtcctggcg cgctgagcta cgggacgtcg gggcagggca
     7741 gcctccattt ctacggcatg gagctgttca actcgatagt tggcatcaag accgtgcacg
     7801 tgccgtacaa gggcagcggg ccggcgctca tcgacctgct gtcgggacaa atccagctca
     7861 tgtttaccag cacagtgtcc gcgattccgc atctgcgcag cggcaagctc aaggcggtcg
     7921 cgttcaccgg gcgcaaccgc acgcagatct tcccccaggc gcccacattg tccgaaacgg
     7981 ttgcgccggg attcgagttg aagaacatgt attgcctgtt cgcccccgct aaaacgccga
     8041 aaccggtcct cgctcttctc aaccgggaag tagcggccat cgtcaatgcc ggcgaggtca
     8101 ggaacaagct cgccgccgat ggcgccgagc cggcgccgcc ggtgagcgtc gacgagttca
     8161 ggagcaccta tcaccgtgaa gtcgggacat gggagaaact ggtcaagaac ctgaaggcag
     8221 agatgtgatc gcgagtgccg ggtaattgaa tcggaggtca cgagatgaaa acagtccgtg
     8281 aacgtgctta tgccgtattt ctagcgctgg tgatctcgct tgcgccggct gcgagtctcg
     8341 cccagcagaa ggacacacgc ggcggctatc cgcgcaagcc ggtgcgcgtg ctggtgggct
     8401 cgcctgccgg cagcggcagc gacctcatga tgcgcgccgt cgcccagaag ctgaccgagc
     8461 gctggggcca gtcggtcatc gtggacaacc gcgcgggcgc cgcgggagcg atctcgctcc
     8521 agctcgcgac gcaggcggtg ccggacggct atacgctcgc gaccctcagc gcgcagaacg
     8581 tgaccgcgat gatcctcggg acggtgacga ccgacatccc gaaggagctg acgccggtcg
     8641 tgctgatgat cacccagccg tacctgatgg tcgccacgcc gtcattgccg gccaattcgg
     8701 tgaaggagct gatcgcgctc gcgaaggcga agccgctcgt ctacgcgtcc agcggcattg
     8761 gttcggtggt gcatctcggc atggagctgt tcaaatccat ggcgggcgtc gagatgacgc
     8821 atatcccgta caagggcagc ggcctgtcga tgatcgatgt catgagcggg cgcgtgcagc
     8881 ttgcgatcac gaacacgctg accgccacgc cgctggtgag aggcggcaag ctgaaggcgc
     8941 tcgcgatcac cagcccccag cgggtgcaag ccatgcccga gctgccgacc gtctccgagg
     9001 cgggcgtgcc gggctatgaa ctgcgcagct ggtacggcct tctggtaccg aacaagacac
     9061 cgccgtccct cgcgctcatg ctcaaccgcg aagtcagcga gacgatgaac gcaccggagg
     9121 tgagggacag gctggctgcc gacggggcgg aggcggcggc gccgaatacg ccggcgcagt
     9181 tccgcggcct gatcgccggc gagatcgcca aatgggaaaa gctcatcaag gcgctcggca
     9241 tgcaggcgaa ataaaggacc ttgcaacatg aaaaccggaa cacccatctc gaaagccgcg
     9301 ctctgtgtgc tcctggccgg tgttgcaacg gctgcgcacg gcgagcgccc gtccgggctc
     9361 ccgggaggct acccggccaa ggcggtgcgg gtcatggtcg ggacctcgcc cggcggcggc
     9421 gtggatacga tcacccgcgg tgtggcgcag aagctctccg agcagtgggg ccagtccttt
     9481 gtggtggaaa accgcacggg cggcggcggt gtcctcgccg tgacgctact cgcgcaggcg
     9541 gcgccggacg gctacacgct ctacggcggg ggcagccagg tcgtgaccgc cacgccgctg
     9601 aagaaagtcc ccttcgacac gcgcgtggtg ctccagccgg tggtgcagat gacctcctcg
     9661 tggtacttcc tgcttgtgca cccctcgctg cccgtgaggt cggtcaagga gctcgtcgcg
     9721 ctcgcaaaaa agcggccggg cacgctcaac tacgggtcgg cgggaacggg ttcggcgtcg
     9781 cacctcggtg gcgaactctt caagttcaag actggcatcg aagtgatgca catcccgtac
     9841 aagggcaacg gccaggcgct cattgatctt gtgagcgggc agacccagat gatgttcacg
     9901 agcacgatct ccggctcgcc gcacgtgaga agcggcaagg cgcggggcat cgcggtgacg
     9961 gcgtcaaagc gcctggcggc gtatcccgat gtgccgaccg tttccgagtc cggcgtcccg
    10021 ggcttcgagc tcgacaacat ctacggcatc tacgcgccga cgggcctggc gccggcgatc
    10081 cttggcgcgc tcaaccggga gatcggccag atcatcgcct cgcccgaaat gaggggacgg
    10141 atcaccgcgg acggcgcgga gcccgcgccg ccccattccg ccgcagagtt cagggaccgc
    10201 ttcgcccggg aaatcgggac gtgggagaag ttcatctgga cctcgggaat caaggtggaa
    10261 tgacctcaat gcgcaggagc gcttgatgaa tccggagaca gcgatgaacc cgctggccga
    10321 cagcgatgcc ggccgggacg ccacgcgcaa gctggcgcgc cacgcggcca cgctcgccta
    10381 cgaatcgttg ccgcgggcgc tggttgagct caccaagcaa tgcgtgctcg atacgcttgg
    10441 cgtcacaatc ggcgcaagca gccttgcgcc cgaggcgaag atcgttgccg aatacgtgag
    10501 cgacctcgac ggcaagcccg aggccaccgt gctgggcttc ggcgggaaag cgccggcgcc
    10561 ctgggtggtg ttcgcgaacg gcagcctcgg ccacatgctc gactacgatg atcttggcgg
    10621 cgggcacgtg agcatcgtca ccgtgccggt ggcgctcgcg ctcgcggaaa aagcgggcgg
    10681 catgagcggg cgcgacttga tcacggcgat cgccgccggc gccgacgtaa tgacgcgcct
    10741 caccctcgct atccgcattc ccgactggac catgaccgag ggatggttcg ccacccagct
    10801 gctcggcttc attggtggcg cggccactgc cgggcggctc ctcgggctcg atgaggagca
    10861 gatggagaac gcgctgggca tcggcttcaa ccagatgagc ggcagccgcc agatggcggt
    10921 cggcgccgcg acgcacatgc gctccatgca ggcgggtttc tcggggcagg gcgcggtgct
    10981 cgcggccgag ctcgcgcgtc gcggcatcat cggctccaag gaaatcctcg agggccgcta
    11041 cgggttgttc aagacctacg tccggaccaa tgagccggac tggaacgcca tcctcggcgg
    11101 cctgggcgcc cggttcccgc tgctcgacac gcacggcttc aaggtatggc cggcgtgcgg
    11161 ctacacgcgg ccggtcaacg cggcgctgct gcagctgcgc gaagagctcg gcttgcgccc
    11221 agacgacgtg gagtcgatca gcgtcgtcgg cggcacgcgc gcgacccagc tcctgtcgga
    11281 gccgcttgag cgcaagcgca ggcccgagac gagcatcgac ggcaagtaca gcatcccctt
    11341 caccacggcg gtcatgatgg tgaagggcaa cgtcacgctg ctcgattaca ccgacgaggg
    11401 attgcgcgat cccgccgtgc tggcgatggc cgaccgggtg agctaccgcg ccgggggcgc
    11461 gtttcccgac gtgatcggcg gcagctcgtc ggtgtcgcgc ccgacggtcg agatccgcac
    11521 ccgcgacggg aggatgcatg cgcgcaaggc cgagcacctg cccggtgatc cgaagcagcc
    11581 ggtcaagcgc gagtttctcg aggcgaaatt ccgggactgc gcgcgtttct cggcgaagcc
    11641 ggtggcgacg ggcaacgtgg accgcgccgt cgagatgatc tgggacctcg agaacgtcgc
    11701 cgacgcgacg gaggtcgtcc ggctgctggc gtagagcggg gaaccaacgc ggaaaaggag
    11761 cgagacatgg cacaaggttc gtccgaagtc acttttatcg gcaccggcga ctgcggcccg
    11821 gtgcacgggc cgcgcgaggg attcccgatc gagcgctaca cggaactcgt ccgcccggtg
    11881 ctcgagaagg tggatttgcg attcggcaac tgcgagcgcc agtactcctc gcgcggtacc
    11941 gccagcgagc gtgcgccgca cggccgccag ccgccggaga tggcgaaaat cttcaccgac
    12001 tgcggcttcg atgcggtgac catcgccaac aaccacatgt atgactacgg ccccgacgcg
    12061 ctgctcgaca cccgcgtgct gctgctggag aaaggcatca aggtgacggg ggccggcaag
    12121 aacctcgatg aagcgcgcga gccggcgatc gtggagaaga acggcgtcaa agtcggcttt
    12181 ctaggctact gctcggtgat ccccgagggc ggcgaggcgg gcccgggcaa ggtcggcatt
    12241 gcgccgctgc gcgtgagaac ctattacgag ccgcgcgggc cgcatgcgcc ggtgcgggtg
    12301 ctcaccgagc cggatgagca ggatatccgc atgatcctcg acgacatcgc cgcgctgcgc
    12361 aagcgggtcg acatcgtgat cccggtgttc cactggggcg tgatctgggt gccgcgggtg
    12421 atcgcggact accaggtcac cgtcgcgcac gcctgcatcg acgccggcgc cgacatggtg
    12481 cttgggcacc acgcgcacgt gcccaaggcg atcgagatgt acaggggcag ggcgatcttc
    12541 tacagcctga gcaatttctg catgaccaag ccgtttccct cgccgaagtg gaaggaagag
    12601 ccgtggaagc acggcgcgct gcgcaaccat gccgaccagg atcccgacat gccgctgctc
    12661 ccgtacggcc gcgacgcgaa gcgctcgctg cttgccaagg cggtctttac caagtccggc
    12721 gtcaggcggg tctcgttcct gccgatgatg atcgacaagc tctaccgccc ggaggtcctg
    12781 cgcggcggcg acccgcgctt cgaggacatg gtgcgctaca tggagtgggc gtcggaaggg
    12841 tttgaccacc acttcgagat cgctggcgat gaagtcatcg tcaccgcgca acccgcggcc
    12901 ccggcgcgga aggaggccgc ttgagcgacg ccggtctcaa atcggacggt cttcagcagc
    12961 gcctcgcgga ctacgcgcgc gccctgacct acgacgcgct gaccccggcg gcggcgcacg
    13021 ccgcgaaagt gcgcgtgatc gacaccctcg cggcgctgat cggcggcttt gacggcgagc
    13081 cttgccgcat cgcccgcgcg atggcggccc gcgcgccgct cgccgacggc gcaacggttt
    13141 acggcacggc gacccggacc acgcccgaga tggcggcttt cgtcaacgcg acggccgccc
    13201 gctacgtgga gctgaacgac gtctatcact ggccgggcag cgccggcggc catccgagcg
    13261 atgtgatcat gccgctgctc ggcgtggccg agtatgcgcg cgcgaccgga cgcgatctca
    13321 tcaccgcggt cgtactcgcg tacgaaatct acctgcgcct ttccgatgca atcggccgga
    13381 cggcgttcga ctgcgccaat ttcgcctgcc tcggcgtcgc ggcaggctcg gcgcggctcc
    13441 tgggcctcgc gccggaacag ctcgcgcata gcctgtcgat ggcggtcgtc ccgaacaaca
    13501 tcctgcggca ggtccggacc ggacacctat ccatgtggaa ggcggtgacg gccggtcatg
    13561 cgggcaaggc gggcgttttc gccgcgctgc tggcgcgcga aggtatgaac gggccgttcc
    13621 tgccgttcga ggggagggcg ggctggtgcg accacatcgc gggcaggcgt ttctcgctcg
    13681 atgcaatggg cgggaacggc gcgcctttca agatccagga cacgatgatc aagccgcgcg
    13741 catcgtgcgc cacgacgatc tcctcaatcc ttgcggccga gaaggtggcg ccggcggttc
    13801 gcggccgcat cgcggaggtc gagcgcgtga cggtggaaac ctacgagcgg gccaaggccg
    13861 gcatgggcac cggcgagcac cactggaacc cggattcgcg ggagaccgcc gaccacagca
    13921 ttccctacgt ggtcgccgcc gcgttgatgg acgggacggt gaccccgcgg cagttcgacg
    13981 aagcgcacct gcacgacccc ggactgcacg cgctgctcgg gaaaatcgac gtcgtggcga
    14041 acgaggaatt cacgcgcgcc tacgagaggc tgccggtgga gcatcgcacg cgggtcaacg
    14101 tcgtgatgcg cggcggcgag cgtttcaccg gcgaagcggg cggcgacaaa ggcgatcttt
    14161 cggagcacaa gagcgacgcg cagatcgcgg agaaatttct cggcgtcacg gaagctttcc
    14221 tgggcgcgcg gcgcgcgagg gcgacactcg cgcggttatg ggagctggac ggtctcgatg
    14281 acgtcgcgcg gcttccggag ggttttgttc aaggctgaca aggagcgcag ggaatggcca
    14341 agaatggctt caagattttc gattccgaca ctcacgtcgg cccctacgtc gacgtgctgg
    14401 agaagtacct gaccgaggca gaaaagtcga agctgcccgc atgggagcag tacaagtcgt
    14461 ctaccagccg cggctacgcg atctacgcca ggggccaacg gcactaccgc cgccggctgg
    14521 gcgctgccga tccccaggac acgcgcggca agtacatggc cggattcacc ggcgcgcacg
    14581 gcgcgaggaa accgagcccg cgcgtggact atgaccccgc cgagcgcatc gccgacatgg
    14641 actacgaggg cgtggacgtc aatctgatgc tgccctccgg ctggttcggc acctggacgg
    14701 cgagcgacga cgtggcgctg gagatgggca tgtaccgtgc ctaccaccgc tggatggagg
    14761 attattgctc cgcctatccc gagcggctgg gcggcgtgat cctcgcttgc gcgcgcgaca
    14821 tcgagggcag cctcgcggag attcgccgct ggggtaaatc gcgctgggcg tgggggctga
    14881 tggtgtacgc gccgcacggc atgccgctcg atcaccccga tctcgagccg gtgtgggccg
    14941 ccgcgcagga ccacgacctt tcgatcgcgc tgcacacctt caccgtcatg ccgccgtacg
    15001 cgccgggcgg gctcgacacc tgggacaacc tgttcctgca gcgctccgcc gcgcacccct
    15061 ggtgcggcat gcgcaacatg gccgccctga tcggcgccgg tgtgatggac cgttacccgc
    15121 ggcttcgaat cggcacgctc gaagcggggc acggctggct gccgttctgg atggagcgac
    15181 tgaacgagca cgccaggacg atccgttccg cgctaccccg gttgaagcaa acgccgggcg
    15241 aatacgtcac cggcgggcgc tatttccaga gcatcgaggc cccggagggc gcgaagctca
    15301 ccaacgcggt gatcgacctc atcggcgagg aggtgctgat gtacgcctcc gattatccgc
    15361 acggggagag ccattttccc cacacggtgg agatggtgat gggctgggaa atgccggagg
    15421 cgcgcaaacg caagctgttc tgggacaacg cgattcgcta ttacgcgcgc tgcgggttga
    15481 aatagcgacg ccgcgcgatc aagaacccgt acgaggagaa agaccatgaa acatcgccgt
    15541 gtgacggcag tgctggccgc ggcggcagcg ctgtcgccct gcgcggtctc cgcgcagagt
    15601 ccggctgatt cttatcccgt caaacccgtc cgggtgatcg tcgggctcgc acccggcggc
    15661 ggcaccgaca tccagacgcg gctgctcgct caaaagctca gcagcaatct cggccgttcc
    15721 ttcgtcgtcg agaaccgcac cggcgcgggc ggcacggtcg cctacgcgct ggtggcaaag
    15781 tcgcctgccg acggctatac gctgctcggc gtcgcgagcg gctattcgat cacgcccgcg
    15841 gtctatccaa agctggcgta cgacccgatc aaggatttcg ccccgatctc gctcgtggtc
    15901 caggcgccgc tcctgttgat gacgcatcct tcgctgccgg tgaggtccac gcgcgagctg
    15961 gtcgcgctgg cgaaacggaa gccgggcatg ctggatagcg cttctgcggg gcacggcact
    16021 tccaaccaca tggcgctgga actcttcaac ctgctcgccg gcgtcaagat cacccatgtg
    16081 ccgtacaagg gtaccgggcc cgctctcatc gacgcgatgg cgggccaggt gcacatgatc
    16141 ttcggcaaca tcctgtcatc cctgcagtac gtgcgccagg gccggctgaa agcgctggcg
    16201 gtgacgagcg ccacgcgctc gaaagcggtt cccgagctcc ccaccatcgc ggaatcgggg
    16261 gttcccggtt acgagacgac cacctggcac ggctggctcg ctcccgccgg cacgtccgcc
    16321 gcgatcgtca accggatgag cgtggagctc gcgagggccg tgaaatcgcc cgacgtgcag
    16381 ggccacctcg caggcgacgg cggcgaggcg atcggaagcg gccccgagca gttcgcccag
    16441 catatcgcga cggagatcgc gcgctggcgc aaggtcgtcc aggcatccgg catgcgcgtc
    16501 gagtgagacg ccgggagcgg gaatgacgcg acagcattcg ttcgcggccg cgtggattgt
    16561 ggcggcagcc gttggactgc cgggcgcggt ttccgcgcag cgaaccgatg gctttccgac
    16621 caagccggtg cgcgtcgtcg ttccccaggc gccgggcggc gcgaccgaca tccaggcgcg
    16681 cctgttcgcg gtcaggatgg gccagaacct gggccagcaa ttcatcgtcg ataaccgcgc
    16741 gggaggagga gcggcgggcg tggtggcgtt ttccacggtg gccagggcta cgccggacgg
    16801 ccatacgttg ctcgcgatca ttcccagctt tacgttttcg ccggcgttgt acaagaacta
    16861 tccggtcgac ccggtgaagg atttcgcgcc aatctcgctt ttgacgcggg ccccgtacct
    16921 gctggtggtc aacgcttcga tgccggcaaa gtcagtgaag gaattcatcg cgcatgcgag
    16981 agcgcagccc gagggtccgc tttttggcgc gggcaacacc ggcagcggca cccatctcgt
    17041 gaccatgtgg ttccttaccg cggcgaacgt cagggcttcc tacgttccct accgcagcgt
    17101 agggctcgcc atgctcgacc tgatcggcgg gaggatcgat gcgacgctgg cgaacgtctt
    17161 gagcgttggg ccgtacgtga gagccggcaa attgcgcgcc ctaggaatca gcaccgcccg
    17221 gcgttccagg gtgtatccgg acctgccgac catcgcggaa caaggcgtgc ccggctatga
    17281 cgcgtccacg ttccacggct atgccgcgcc ggcgggcacg cccgcggccg tcgtgaacaa
    17341 gctcagcgcg gaattcgcga aggtcgtcaa gtcttctgac gtggcagaga gaatttccga
    17401 agacggcggc gagcccatag gcggcacgcc aaaggaattc cggcagttca tcgccgcgga
    17461 aattctcgtc tggcacaagg tcgtgaagga cgccggcata tccgtgcagt agggcggcga
    17521 tccgatcaac gccgcgcgga agaacgtgtt atcgttgtcg ccttctcaat caaggcaagg
    17581 cgaagacatg ctgaaggcgg cgatctacgg aatgggccgg tggggaaccc ggctggtcga
    17641 gtcggtgcag ccgagcgaga agatccgcat cgtcaagggc atctcgcgcg atcccgaaaa
    17701 acaccgcgag ttttccgaga agaccggcat ccgcttcatc tcctcctacg gcagggtgtt
    17761 gaaggacccg gagatcggcg ccgtggtgct cgcgaccccg cactcgctgc actggaagca
    17821 gatcgtccag gcggcgaagg cgggcaagca cgtcttcgtc gagaagccgc tcacgctcac
    17881 gcgcaa
//
LOCUS       DBNGWZ010000413        17987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_804492, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000413 DBNGWZ010000000
VERSION     DBNGWZ010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_804492"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..150
                     /locus_tag="AC16CX_12385"
     CDS             <1..150
                     /locus_tag="AC16CX_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3013344.1"
                     /translation="EEIINNAEKRKCDLIMMASHGRRGIDALLLGSETAKVLTHSKIP
                     VLVVR"
     gene            291..527
                     /gene="rpmB"
                     /locus_tag="AC16CX_12390"
     CDS             291..527
                     /gene="rpmB"
                     /locus_tag="AC16CX_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3013345.1"
                     /translation="MSRVCQVTGKKPMLGNNVSHANNKTRRRFLPNLQYRRFWVEAEN
                     RWVSLRISQAGLRTIDRKGIEAVLAEMRARGEEI"
     gene            538..693
                     /gene="rpmG"
                     /locus_tag="AC16CX_12395"
     CDS             538..693
                     /gene="rpmG"
                     /locus_tag="AC16CX_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3013346.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKRTTPDKMEIKKYDPVARKHVI
                     YKETKIK"
     gene            complement(871..2034)
                     /locus_tag="AC16CX_12400"
     CDS             complement(871..2034)
                     /locus_tag="AC16CX_12400"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR3013347.1"
                     /translation="MFTGVWDLPWWGYVIYTLAITHVTIAAVTIFLHRCQAHRALDLH
                     PVISHFFRFWLWLTTGMITKAWTAIHRKHHARCETEEDPHSPQILGINKVLWEGAELY
                     RKEALNAETLDKYGRGTPDDWIERNVYAKRDTFGIYVLMAVNFALFGFIGLTIWAVQM
                     AWIPFLAAGVINGIGHYWGYRNFQPEDASTNIVPWGILIGGEELHNNHHAYASSARFS
                     NRWYEFDLGWYYIRGLALLGLAQIKKVAPEIKLDTAKTRCDLATVQAVITHRYDVLAK
                     YANSLRQTCADEIRHLKTRAVSVDRAAIKRMLHAGADALPANERAKISEALSKSKVLA
                     TVCTMRQELVSLWQRSSVSKEQLVRQLEDWCARAEASGIIALQEFSRRLRCYA"
     gene            2214..5060
                     /locus_tag="AC16CX_12405"
     CDS             2214..5060
                     /locus_tag="AC16CX_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3013348.1"
                     /translation="MTPRAILGPTRAAMVQRALRGGRTVQGMNLALRLRNLEPPRRCW
                     RAGPPAHVPVRMRQACSERRRMNEYDPLTRDELISLIRELKADPRRERGPVPGNPEGF
                     PVFGEQQLRQFEASPHAMRIAEHGTLRYLAVNAAALKLYGYNREEFLKLTLRDTRRPD
                     EHAEQAASFAAPTGSLRHSAIRRHIKKSGEIIFVEVITQDILFDGRPARLSLTVDVTA
                     RLRVQELLWQRQQEFESLAENLPDLVARFDRERRYVYVNAAVEKLTGKARDDIIGRTQ
                     RELGMPDDLITTFEESLTDVLSAGRTHSLEFRFPAPDGERLFEAFHVPERDQTGEIAT
                     VLCVARDITRRKRTHDALRESREQYRAVTETANDAIVSADGNGNIIYFNQAASRTFGY
                     AVEEAIGRPLTLLMPARFHADHKAGLRRFLDGQTPTMIGKTMELVGRRKDGTEFPVEV
                     SLASWRTSASVFFTGILRDITERKRAAQALSETNEFLRSVIDSSRDCIKVLHLDGRLQ
                     WISTGGQRLMETDDEAPFLNRSYLDFWSGAERELARNALAAACSGSTSNFEGYCPTVK
                     GTPKWWDEIMTPILDAEGKPEKVLVVSRDITRRRAVENALRESEERFRQLAENIREVF
                     WISHPNGDNIYVSPAYEEIWDRSRESLYRNPRSWMEPIHPDDLPSVQECQKKMARGQL
                     TDVEYRITRPDGTPRWIRDRSYPMKRDDGTRLVCGIAEDITNRKRAEEERLAHAILQR
                     DALVREVHHRIKNSLQGIVGLLRQRIRNKPAIAPEIEETIAQLQSVALVYGLQETRPD
                     GLLSLADITEAICSSAESLIGGRVERAFERKSQRPACLAGAEAVSVAVALNELVFNAL
                     KHQPAAAGKKRARLVLREAKDEAEIRISNRGRLPKGFDFAENRAVGSGLGLVRTLLAP
                     PGGSIAFNGGRNEVEVVLKLQPPLLAERQTMGGR"
     gene            5068..5721
                     /locus_tag="AC16CX_12410"
     CDS             5068..5721
                     /locus_tag="AC16CX_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR3013349.1"
                     /translation="MATAQAGKETRRHILVVDDDRLVLAALAEGLRATGYRVTGAVNG
                     EDALRAAARDLPDLALLDVRMPGMNGIELGRRLRERTGTPFLYLSAYGDRDIVQQAAE
                     EGALGYLVKPLDIQQIVPSIEAALKRAREIRTLRDKETQLTSALAGSREISMAVGLLM
                     MRDSLNRKQAFELLRSHSRSQRRQVAEVAKALLGSAENLYTIKDPRAPSRRRKQRDT"
     gene            complement(5808..6671)
                     /locus_tag="AC16CX_12415"
     CDS             complement(5808..6671)
                     /locus_tag="AC16CX_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013350.1"
                     /translation="MRVLVNGVRLYFDVEGAGLVPDGSVMREKPTLLLLHGGPGMDHS
                     TYKPRFSQLADIAQVIYLDHRGNGRSDRGPRETWTLAQWGDDVHAFCEALGIARPIVM
                     GTSFGGMVALAYATRHPEHPAKLILASTEAAGGRHLERRVALFERLGGMEVGALARRR
                     LIDGRLDEAILEEWVRLAFPVYTRTRRDPAWVERAIRNPEVHMWFMRPGGEGLTFNLL
                     PVLRRVQCPTLVLGGEEDPMIPIVCQEEIVAALPARLARFERFPGCGHGVINDAPERA
                     MAVIREFITAR"
     gene            7239..8150
                     /locus_tag="AC16CX_12420"
     CDS             7239..8150
                     /locus_tag="AC16CX_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HIR3013351.1"
                     /translation="MQARVTTKPEQEVIAAIQGLNLESVKMRVMDPELGEGWTREYAD
                     NIEMAYKTYLTMLVKYQDHAEDILLSKDVDEFWHTHILQTMKYEDDCQSIFGRFLHHQ
                     PHIGELTQADHEKRTLLAEKTLRLYQREFGNEQDSAVAWSGMPVRAENSETSEAAVRA
                     ESAAVSGVAIRRESAAVSGVAIRAESAAVSGVAIRRENAAVSGVAIRAEGAAVSGVAI
                     RKENAAVSGVAIRAESAAVSGVAIRKENAAVSGVAIRAEGAAVSGVAIRKENAVVSGV
                     AIRAESAAVSGVAIRKENAAVSGVAIR"
     gene            8439..10286
                     /locus_tag="AC16CX_12425"
     CDS             8439..10286
                     /locus_tag="AC16CX_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HIR3013352.1"
                     /translation="MEYMPLAAPKAGRWALYSSFLPWLVLAIGIPASVFLYVLIQDSI
                     ENVARLRFEREAQDASSVIQGRLRSYSDVLYALRALFASESPVNRLRFHRYLDSLNLK
                     LRYPGFISLNYAAYVRNSDKRRFEEAVRNDTSLNPRGYPQFSIKPPGERPEYFVMVYL
                     EPVANYEFAFGLDLGMNPSAENPGKVAAAMRLGRDSGELLSSAQPLRIKVRKETIYLS
                     MRLAVYQNGMPVDTVDQRRAAYLGSVGAGFDVESLIREALSEELLRYIRLRLYDIGSS
                     IDEGDTKPPGGKRLLFDSDRVTAHSSKETAADDFSMAFVHALPIEIAGRVWEFEYSAR
                     KSAIISETDRILPPVILVGGLLLSALLFGVLYSLASSRRRAVEIANEITRDLRESEKE
                     LRATAEQLQAMSRRLVEIQESERRQFSRELHDRVGQNLTALSISLDILKSQLSNNGNE
                     ALRSRLDDAATLLESTAGAIENVMSELRPPMLDDYGLLPALQWYANEFSGRTGIRVRV
                     EGDERMERLTQASEIALFRIVQEALNNVAKHAHAGRVHIALERSGAQLVMSVSDDGVG
                     FDAATTPASRRRPGLGMVTMRERTQAVGGRFEIGPASGRGTRVVVRVPC"
     gene            10288..10932
                     /locus_tag="AC16CX_12430"
     CDS             10288..10932
                     /locus_tag="AC16CX_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013353.1"
                     /translation="MAIRVLIADDHAVVAEGLRFIVEAQADMEVIACVQESREAVRRA
                     IEMRPDVVLIDHAMPVLNGAEATHLIRERCPETRVIILSMYSNRVHVLRALQAGATGY
                     VVKKSAAKEVVDAIRAVHKGGRYLSREVADSVIDQVVGKAATDDPMKRLSSRERQVLQ
                     MLVEGHAVADIATTLSLSPKTVETYRARMMEKLEIHDFATLVKFAIQHGITSLE"
     gene            11289..11681
                     /locus_tag="AC16CX_12435"
     CDS             11289..11681
                     /locus_tag="AC16CX_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013354.1"
                     /translation="MNTDSPQGPIALVVEDNDTVRRALCDRIHTSFAQFRLREAGTVD
                     DALKIVESERVDLVLMDFRLPGRNGVDGTRMVLERSPHTSVVMVSVFDDSSHRSAARQ
                     AGARAFVSKRAIGRELIPVIESLVNCGQ"
     gene            11669..12337
                     /locus_tag="AC16CX_12440"
     CDS             11669..12337
                     /locus_tag="AC16CX_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR3013355.1"
                     /translation="MRAIAEMAARDNIETQQHILVVDDDRLVLAALAEGLRDAGYRVT
                     GAVSGEDALEIVGRDAPNLALLDVRMPGMDGIELGRRLREQANVPFLYLTAYGDWDIV
                     KQAAEQGALGYLVKPLDIHQIVPSIEAALRRAREIKKLREREAQLNAALAGTREISMA
                     VGLLMMRDRLDREQAFELLRFHARSQRRTVAELARELLSSAENLNVVSKLKQNAPRGK
                     RGNT"
     gene            12734..14494
                     /locus_tag="AC16CX_12445"
     CDS             12734..14494
                     /locus_tag="AC16CX_12445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013356.1"
                     /translation="MPVTDMIHRATNNSESVRRFMSHVHTRWGYAIVFSLTLLAIIVT
                     AVQSYRAIDRELTEVALSRRIAVAQLAAATLSEKLGRLVDVTLSLATRVRFRQLVASG
                     EWGEAIKILRDVPRDFPFTDRLVLFDARGTVMADIPEQLEVRGTNFAYRDWYQGVSRD
                     WKPYVSTVYRRAAAPQSNVFAVAVPVRRDSDGEVAGILLLQTRLDAYFEWVRDAEVGP
                     SGFVYVVDQKGQVAFHPKFPPQGTIVDLAAVPVVQRVLRGDRGIEIAFDSTEKEDAIS
                     AYAPVAKYGWGVIVRQPKHAAFAAKDQQIERLLIAYGLTFLFFVLGIYLASRIVIQRR
                     QAEEDRRMKAELERMVAERTAQLEVSNKELESFSYSVSHDLRSPLRAIDGFSRILQEE
                     HGDKLGDEARRLLGIIRDNSQKMGRLIDDLLKYSRLGRKPLSTAEIDMKQLAEEVFGE
                     LQAGNERRPKLMLGVLPLAHGDATLVKQAWVNLLDNAIKFSSKREQPMIEVSGYGNSA
                     ESVFCVKDNGAGFDMRYHDKLFGVFQRLHAADEFPGTGVGLAIVHRVVTRHGGRVWAE
                     GKVGEGAAFYFALPGRVRDD"
     gene            14487..15974
                     /locus_tag="AC16CX_12450"
     CDS             14487..15974
                     /locus_tag="AC16CX_12450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013357.1"
                     /translation="MTEPVRLSPPLPAKAPGRFGLPASLVGILLPFAAFAVQWTFWDA
                     ISPYVWFLFYPTVFFAPLFGGLWSGIVATVISTLLVWYVFIPTAFSFRIEDPISIVSI
                     ALFTLMGVIFSLFHEHLRKLGLKVAQQESNERFRRALDNMMEGCQIIGFDWRYLYVNA
                     TAAKQGRSTVAELTGRTMMEAYPGVETTAAFAALKRCMEARLPEQMENEFVYPDGTLA
                     WFQLAIQPVPEGVFVVSLDITRRKRAEEEIRTLNAELEERVAARTAQLEFANRELEGF
                     AYSVSHDMRTPLRAIDGFSRIFQEDYAGKLDDEARRLLGVIRDSSRKMGQLIDDLLEY
                     SRLSRKTLSTSGINMTRMVEEALEELRTAGGRWPELVLGALPPACGDAALVKQVWLNL
                     LANAIKFSSKRERPVIEVSGREDDAESVYCVKDNGIGFDMQYYDKLFGVFQRLHGVEE
                     FEGTGVGLAIAQRVIARHGGRVWADGKVGEGAAFCFSLPRGAQHG"
     gene            15967..16404
                     /locus_tag="AC16CX_12455"
     CDS             15967..16404
                     /locus_tag="AC16CX_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013358.1"
                     /translation="MDKFEKVDVLLIEDNPTDAELTIRALRKGRLANNVTWVKDGAEA
                     LEFIFRNGAYAGRPDDNPRLILLDLKLPKVDGIEVLKRVKADERTRAIPVVMVTSSQE
                     GRDIIESYKLGVNSYVVKPVEFEQFSETVAKAGFYWMLVNKAP"
     gene            16435..>17987
                     /locus_tag="AC16CX_12460"
     CDS             16435..>17987
                     /locus_tag="AC16CX_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR3013359.1"
                     /translation="MAKELRILMIEDVPADAELEIRELKRAGLRLAHRLVETEAAFRD
                     ALRDFPPDVIISDFSMPHFDGMWALELARVLAPDVPFIFVSGTIGEEYAIRALKSGAT
                     DYVLKDNLVRLPPAVERALQDSAERAARRKTERELEETRSRLDSIVTSLSDVVWSFSL
                     RENRMLYVSSAARNMYRQPVNTFYAEPMAWMDAIHPEDRERVMQEWNASIAARRFDST
                     YRIVLPDGEELWLHNRGNLITDEQDAPVRIDGLARDITERKRDEEALRESESRLDLAL
                     QASGLATWEWNLTTGEVRFSRHWWPILGYGSDEVPLRVEAWEKLTHPDDLQKVKSMLA
                     SAVKGSDPVLDIEYRMRAKSGEWCWIRTVGQVVERDATGRTSRMTGTHGDVTERKRAE
                     EKIRRLNRVYAVLSGINSAIVRIRDRQELFEEACRIAVEGGNFGMAWIGTLDPAAQEV
                     TPVAWAGLGAGDYLGGIKSRIRDDLPTGRGVLAQALKTRKPVFDNDITINPEMGGARR
                     QEAIRRGYRS"
BASE COUNT         3585 a         5255 c         5844 g         3303 t
ORIGIN      
        1 gaggagatca tcaacaacgc cgagaagcgc aaatgcgatc tgatcatgat ggcctcgcac
       61 gggcgcaggg gtatcgatgc cttgctgctc ggcagcgaaa ccgccaaagt gctgactcac
      121 tcgaaaatac ccgtgctggt cgtgaggtag ggcaccgaag ttccaagtta caagttcaaa
      181 gtcgaaacat cgaatcgcaa cccgctccaa ccttgaacct tgaatcggcg cttgagccgg
      241 gggcaaaaac cctgtagaat ccgcgccttt cgagatttcc ggagccttcc atgtcacgcg
      301 tctgccaggt caccggcaaa aagccgatgc tggggaacaa cgtgtcccac gccaacaaca
      361 agacgcggcg gcgcttcctg ccgaacctgc agtaccgccg tttctgggtc gaagccgaga
      421 accgctgggt cagtctcagg atctcgcagg cggggctgcg aacgatcgac cgcaagggga
      481 tcgaggccgt gctggccgag atgcgcgcgc gcggcgagga aatctgagga gcccgtcatg
      541 cgcgagaaaa tcaagctcga atccaccgcc ggcaccggcc acttctacac caccaccaag
      601 aacaagcgca ccacacccga caagatggaa atcaagaagt acgacccggt ggcgcgcaag
      661 cacgtcatct acaaggaaac aaaaatcaag taaatccgga cggctttcgc cgccggttta
      721 cctcctcgcg ttcgaacggc cggtaaaccg ccgttcaacg ctcagcgatc acagaacaag
      781 gattagggaa taaaaaagcc cgcttgaaag cgggcttttt gctttttcag aatttgctaa
      841 attctcaatc ctcggtcctc aatcctgtat ctaggcgtaa cagcgtagcc ggcgcgagaa
      901 ttcctgaagc gcgatgatgc cgctcgcttc cgcacgcgcg caccaatctt cgagctgacg
      961 cacgagttgc tccttggata cgctcgagcg ctgccacagt gacaccagct cctggcgcat
     1021 ggtgcagacg gtggcgagca ccttgctctt gctcaacgcc tcgctgatct tggcgcgctc
     1081 gttggccggc agcgcgtcgg cgccggcgtg cagcatgcgc ttgatcgccg cgcggtccac
     1141 gctcacggcg cgcgtcttga ggtgacggat ctcgtcggcg caggtctggc gcagcgagtt
     1201 cgcgtacttg gcgagcacat cgtaacgatg ggtgatcacg gcctggaccg tggcgagatc
     1261 gcagcgggtc ttggcggtat cgagcttgat ctcgggcgcg accttcttga tctgcgccag
     1321 ccccagtaac gccagccccc ggatgtagta ccagccgaga tcgaactcgt accagcggtt
     1381 ggagaagcgc gcggagctcg cgtaggcatg gtgattgtta tgcaactcct cgccaccgat
     1441 caggatgccc cacggcacga tgttagtgct cgcgtcctcc ggctggaaat tgcggtagcc
     1501 ccagtaatgc ccgatgccgt tgatgacgcc ggcggccaag aacggtatcc acgccatttg
     1561 caccgcccag atggtgagcc cgatgaagcc gaacagcgcg aaattgaccg ccatcagcac
     1621 gtagatccca aacgtgtcgc gcttcgcgta cacgttgcgc tcgatccagt catccggcgt
     1681 gccgcggccg tacttatcga gcgtctcggc gttcagcgcc tccttgcggt agagctcggc
     1741 gccttcccac agcaccttgt tgatgcccag gatctgcggg ctgtgcggat cttcctcggt
     1801 ttcgcacctg gcgtggtgtt tgcggtggat cgcggtccac gccttggtga tcatgcccgt
     1861 ggtgagccat agccagaaac ggaaaaaatg gctgatgacg ggatgcagat cgagcgcgcg
     1921 gtgcgcctgg cagcggtgca ggaaaatggt gactgcggcg atggtaacgt gagtgatggc
     1981 cagcgtgtaa atgacatagc cccaccatgg aagatcccag acccctgtga acattggaag
     2041 atcccagacc cctgtgaaca ttggcccccc tctagggaag tttttagtgt gcccggtgct
     2101 acgggttggc gcagtctacc gaaattcatc cccgaggcat agcgtttttt gacgcacccc
     2161 gggccgggct cgcgctgtag cgtactgatt aaaaagccaa acttcggaat ccagtgacgc
     2221 cgcgcgcgat cctgggacca actcgcgcgg cgatggttca aagggcgctt cgagggggtc
     2281 gaaccgtcca ggggatgaac cttgcgttgc ggctgcggaa tctggaacca cctcgccgct
     2341 gctggcgagc cggtccaccg gcgcatgtac ccgttagaat gcgtcaggca tgctcggagc
     2401 gacggcggat gaacgagtac gaccccctca cgcgcgatga gctgatctcc ctcatacgcg
     2461 agctcaaagc ggacccgagg cgcgaacgcg gcccggtgcc cggaaatccg gagggcttcc
     2521 cggttttcgg cgagcaacaa ctgcggcagt tcgaggcgag cccgcatgcc atgcgtatag
     2581 ccgagcacgg cactttgaga tatctcgcgg tcaacgccgc cgccctgaag ctctacggct
     2641 ataaccgcga ggagttcctc aagcttacgc tgagggatac gcggcgtccg gacgaacatg
     2701 ccgagcaggc cgcgagtttc gccgcaccca caggcagcct caggcattcg gcaatccgcc
     2761 gccacatcaa gaaatccggc gagatcatct tcgtggaggt cattactcag gatatcctgt
     2821 tcgacggacg ccccgcgcgt ctgagcctga cggtggatgt gacggcgcgc cttcgcgtgc
     2881 aggagttgct atggcagcgc cagcaggaat tcgaatcact ggccgaaaat ctgcctgacc
     2941 tggtagcgcg cttcgatcgg gagcggcgtt atgtctacgt caacgctgcc gtcgagaagc
     3001 ttaccggcaa ggctcgcgac gacatcatcg gcaggacaca gcgcgagctg ggaatgccgg
     3061 atgacctgat taccacgttt gaggaaagtc tgaccgacgt gttatcggcc ggccggactc
     3121 actcgctcga gttccggttt ccagcgccgg acggcgaacg actgttcgag gcctttcacg
     3181 tgccggagcg tgaccagacc ggtgagattg ccaccgtgct gtgcgtcgcg cgcgacatca
     3241 cgagacgcaa gcgcacccac gacgcgttgc gcgagagccg ggagcagtat cgggccgtta
     3301 ctgaaaccgc caacgacgcc attgtttcgg cagatggaaa cggaaatatc atctatttca
     3361 atcaggcggc gtcgaggact ttcgggtatg ccgtcgaaga agctatcggc cggccgctta
     3421 cgctcctgat gcctgcccgc ttccatgcag atcacaaggc gggcctgcgg aggtttctgg
     3481 acggacagac gccaaccatg attggaaaga cgatggagct ggtgggacgg cggaaagacg
     3541 gcaccgaatt tccggtggaa gtatctttgg ccagctggcg caccagcgcc agcgtcttct
     3601 ttaccggcat cctccgtgat atcaccgagc gcaagcgtgc cgcgcaggcg ttgagcgaga
     3661 ccaatgagtt tctccgcagc gtgatcgata gcagccgcga ctgcatcaag gtgctccatc
     3721 tcgacgggcg tctgcaatgg ataagcaccg gcggccaacg cctgatggag accgacgatg
     3781 aggcgccgtt cctgaacaga tcgtatctcg atttctggag cggcgccgag cgcgagttgg
     3841 cgcgcaacgc attagcggcc gcatgctcag gaagcacgag taacttcgag ggttattgcc
     3901 ccacggtgaa aggcacgcct aagtggtggg atgagatcat gacacccatt ctggacgcgg
     3961 agggcaagcc ggagaaagtg ctcgttgttt cgcgcgatat caccagacgc cgggcagtgg
     4021 aaaacgcgct gcgggaaagc gaggaacgct tccgtcagct cgcggagaac atccgggagg
     4081 tgttctggat cagccatcct aatggcgaca acatttacgt aagtccggcc tacgaagaga
     4141 tctgggacag gagccgcgaa tcactgtatc gcaacccccg cagctggatg gaaccgatcc
     4201 atcccgatga cctgccgtcc gttcaggagt gtcagaagaa gatggcgcgc ggccagctca
     4261 ccgatgtcga gtaccgcatc acccgcccgg acgggacgcc gcgctggatc cgggatcgtt
     4321 cctatccaat gaagcgcgac gacgggactc ggctcgtgtg cggcatcgcg gaagatatca
     4381 ccaaccggaa gcgggcggag gaggaacggc ttgcccatgc gatactccaa cgcgacgcgc
     4441 tggtgcggga agttcatcac aggatcaaga atagcctgca gggcatcgtc ggactgttgc
     4501 gccagagaat caggaacaaa cccgcgatag cacccgaaat cgaggaaacg attgcacaac
     4561 tgcagtcggt ggcgctggtt tacgggctgc aggaaacgcg cccggacgga ttgctcagcc
     4621 tggccgatat aacggaagcc atctgttcct ccgcggaaag cctgatcggg ggccgcgtgg
     4681 aacgggcatt cgagcgcaaa tcgcagcgcc cggcctgtct cgccggcgcg gaggcggtgt
     4741 cggtggccgt cgcgctcaac gagctggttt tcaacgccct gaaacatcaa ccggctgcgg
     4801 cgggcaagaa gcgggcacgg ctcgtgctgc gcgaagcgaa ggacgaagcg gaaatacgca
     4861 tttccaaccg cgggcgcctg ccgaagggct tcgatttcgc cgaaaaccgc gccgtcggca
     4921 gcggcctggg actggtgcgc acgctgctcg ccccgcctgg cggcagcatc gctttcaacg
     4981 gcggccgcaa cgaagtggag gtcgtgctga agctgcaacc gccgttgctg gcagaacggc
     5041 aaacgatggg cgggagatga gcgcaggatg gcgacggcgc aagccgggaa ggaaacccgg
     5101 cgccacattc tggtagtgga cgacgaccgg ctggttctgg ctgcgctggc tgaaggactg
     5161 cgcgccaccg gctaccgcgt caccggcgcg gtgaacggcg aggatgccct cagggccgca
     5221 gcgcgcgacc tgcccgatct cgcgttgctc gacgtgcgca tgccgggcat gaacggaatc
     5281 gaattgggca ggcggttgcg cgagcggacc ggcacgccgt tcctgtacct ctccgcgtat
     5341 ggtgatcgtg acatcgttca gcaggccgcc gaggaaggcg cgctcggcta tctcgtcaag
     5401 ccgctcgaca tccagcagat cgtgccttca atcgaagcgg cgttgaagcg tgcgcgtgaa
     5461 atcaggacgc tgcgcgacaa ggagacgcaa ctcaccagcg cgctcgccgg atcgcgtgag
     5521 atcagcatgg cggtggggct gctcatgatg cgcgacagcc tcaatcgcaa acaggcgttc
     5581 gagctgctgc ggagccactc ccgttcgcag cggcggcagg tggcggaggt ggcgaaggca
     5641 ttgctggggt ccgctgaaaa tctttatacg atcaaagatc cgagagcacc gtcgcggcgc
     5701 cggaagcagc gggatacctg attcaggttc aaagttcaag gttccgggtt caaggtcctg
     5761 ggtcgaactt tgaacttcga attttgaacg tcgaactccg ccaggcccta tcgagcggtg
     5821 atgaactccc gtatgaccgc catggcccgc tctggcgcat cgttgatcac gccgtgcccg
     5881 cacccgggaa accgctcgaa gcgcgccagg cgcgccggga gcgccgcaac gatctcctcc
     5941 tgacagacga tggggatcat gggatcttcc tcgccgccga gcacgagcgt ggggcactgc
     6001 acacgccgga gcaccggcag cagattgaac gtaagcccct ctccgcccgg ccgcatgaac
     6061 cacatgtgga cttcgggatt ccggatcgcg cgctcaaccc acgccggatc gcgccgggtc
     6121 cgcgtgtaga cagggaacgc caggcggacc cactcctcga ggatcgcctc gtccaggcgc
     6181 ccgtcgatca gacggcgccg cgcgagagcg cccacctcca tgccgcccag ccgctcgaac
     6241 agcgcaaccc gtcgctcgag atgccgcccg cccgcggcct cggtgcttgc cagaatcagc
     6301 ttggcgggat gttcgggatg gcgtgtcgcg tacgccagcg cgaccatgcc gccgaacgac
     6361 gtgcccatga cgatcgggcg cgcgatgccg agcgcctcgc aaaatgcgtg cacgtcgtcg
     6421 ccccattgcg ccagcgtcca ggtttcgcgc gggccccggt cactgcgtcc attgccgcga
     6481 tggtcgaggt aaatgacctg ggcgatgtcg gcgagctgcg agaagcgcgg cttgtaggtg
     6541 gaatggtcca tgcccgggcc cccgtgaagg aggagaagcg tcggcttttc ccgcatcacc
     6601 gagccgtcgg gcacgaggcc ggcgccctcg acgtcaaaat agagccgcac cccgttgacg
     6661 agcacgcgca tatcgttatc ccaggatttt gtcggcttcg aggccggcaa aatccttaat
     6721 cggactttct cctggcatcc tgtcagcctc cggtccgaca gaccggcccg gtgattgccg
     6781 agtttactag aagcggtctc aagccccgcg catccgccga tggacgccca ttcctcgttc
     6841 cttccctctc tcccgatggg ggaagtggag caattgtcac ccctttctta tcaggatttt
     6901 gtcgggccta agtccgacaa aatcctcctc tgtcaggaaa ttcctgacat caattgtcgg
     6961 ctacgcccga ctgcacgcgc ttccctcctt aggtaacttt aagtggaatt gcctgcgccc
     7021 caaccggccg caggtgccgc cggtccgcgc gttacgcgcg ggccccgagc acaagcgatt
     7081 tcggcaaccg aaacgctgaa gaggaggtga tcggtcttcc gaagagagct acccgcaacc
     7141 cattcccatt gcagtgacag cacgacaaca ggccgatgcg gcccgcgcag gggcggccgc
     7201 cagtcctgtc ccgttatttc aagcaaacag gatggaacat gcaagcgaga gtgactacga
     7261 aacccgaaca ggaggtcatt gcagcgatcc aggggcttaa cctcgaatcc gtcaagatga
     7321 gggtgatgga ccccgagctg ggagaaggct ggacgcgcga gtacgccgac aacatcgaga
     7381 tggcgtacaa gacctacctt accatgctcg tgaaatacca agaccacgcg gaggacattc
     7441 tgctcagcaa ggacgtggac gagttctggc acacgcacat cctgcagacg atgaagtatg
     7501 aggatgactg ccagagcata ttcggcaggt tcctgcacca tcagccgcat atcggagaac
     7561 tcactcaggc tgaccacgaa aagaggacct tacttgccga aaagaccctg cgcctgtacc
     7621 agagggaatt cggcaacgag caagactctg ctgtggcgtg gtccggaatg ccggtaaggg
     7681 ccgaaaactc ggagacgtcg gaagcggccg ttcgggccga aagcgcggcc gtgtcaggcg
     7741 ttgccatccg cagggagagt gcggcggtgt cgggcgttgc gattcgtgcc gaaagtgcgg
     7801 ccgtgtcagg cgtggctatc cgcagggaga atgcggcggt atcgggcgtt gcgattcggg
     7861 ctgaaggcgc ggccgtgtca ggagtggcca tccgcaagga gaacgcagca gtatcgggcg
     7921 ttgcgattcg tgccgaaagt gcggccgtgt caggcgtggc tatccgcaag gagaatgcgg
     7981 cggtatcggg cgttgcgatt cgggctgaag gcgcggccgt gtcaggagtg gccatccgca
     8041 aggagaacgc agtagtatcg ggcgttgcga ttcgggctga aagtgcggcc gtatcaggcg
     8101 tggctatccg caaggagaat gccgcggtgt caggcgtagc gatccggtag caagacatga
     8161 gcgggaatga gacccttcca tctaccagac acgcggatct gaaggcctct gtggaaataa
     8221 tggccgtggc cgcttgattc gctatgcgtt tccttcctcg ttgggaaggc caactcgaag
     8281 gctggagaaa tccggccttc tttattgcgt tcgcgcagtc gacagagacc aaggatcgga
     8341 gttggtcggt tgctgcataa ttggagcatg gcgtcgacca acgaccgata tctgtccagc
     8401 cccgcctaat ctcggtacct agttgcactg agacgaagat ggagtacatg cccttagccg
     8461 cccctaaggc aggtcgctgg gcgctctatt caagttttct tccatggctg gtattggcta
     8521 tagggatccc ggcatctgtt tttctttatg tgctgatcca ggattcgatt gagaatgtcg
     8581 cgcggctgcg gttcgagcgt gaagcacagg atgcgagcag cgtcattcaa gggcgccttc
     8641 gttcctacag tgacgttttg tacgcgttgc gggctttgtt cgccagtgaa agtccggtca
     8701 atcggcttcg atttcaccgc tatctggatt cccttaacct gaagctgcga tacccagggt
     8761 tcatctcgct gaattacgcc gcgtacgtac gcaacagtga caagaggcgg ttcgaggaag
     8821 cggtgcgtaa cgacaccagc ctcaacccgc gtggttatcc tcagttttcg atcaagcccc
     8881 ccggggaacg gcccgaatat ttcgtaatgg tgtatctcga gccggtggct aactatgaat
     8941 ttgcgtttgg cctcgacctt gggatgaacc cgtcggctga gaatccggga aaagtggctg
     9001 cagcgatgcg cttggggagg gatagcggag aacttctcag ctctgcgcaa ccgctccgga
     9061 tcaaagtaag gaaggagact atctatttgt caatgcgcct cgcggtatac caaaacggga
     9121 tgccggtcga caccgtggat cagcgacggg ccgcgtattt gggatcggtt ggcgcaggtt
     9181 tcgatgttga aagtcttata cgggaagcac taagcgagga attgctgcgg tacatacgac
     9241 tccggctata cgacatcggg tcttcgatcg acgaaggcga cacaaaaccg ccggggggga
     9301 agaggctatt gttcgatagc gatcgggtga ccgcgcactc ttcaaaagag actgcagcgg
     9361 atgatttcag tatggcattc gtgcacgcat taccgattga gattgctggc cgtgtctggg
     9421 aattcgagta cagcgcgaga aaaagcgcca taatcagtga gaccgataga atattgccgc
     9481 cggttattct ggttggtgga ttgcttttga gcgcgttgct gttcggagtg ctttattcgc
     9541 ttgcgtcttc gcgcaggcgc gcagtcgaga tcgccaacga aatcacccgg gacctgcgag
     9601 agagcgagaa ggaattgcgg gcgactgccg agcagctcca ggcgatgtcg cggcggctgg
     9661 tcgaaataca ggaatccgag cggcggcagt tttcccggga gctccacgat cgggtgggcc
     9721 agaatctcac cgcgctcagc atcagcctcg acatcctgaa gtcgcaactc tcgaacaacg
     9781 ggaacgaggc gttgcgttca cgtctcgacg acgccgccac gctcctcgag tccaccgcgg
     9841 gggccatcga gaacgtgatg tcggaactcc gcccgccgat gctcgacgat tacggattgc
     9901 tgccggcgct gcagtggtac gcgaacgagt tctccggccg caccggcatc cgggtgaggg
     9961 tggagggtga tgagcggatg gagcgactga cgcaggcaag cgagatcgcg ctgttcagga
    10021 tcgtgcagga ggcgctcaac aacgtcgcga agcacgctca cgcagggcgc gtccacatcg
    10081 cgctcgagcg ttcgggcgcg cagctcgtca tgtccgtgtc ggacgacggc gtgggcttcg
    10141 atgcggcgac gaccccggct tcgagacgca ggccgggact gggcatggtg acgatgcggg
    10201 aacggacgca ggcggtcggg ggccgatttg aaatcggacc cgcgtcaggc cgcgggacgc
    10261 gggtcgtggt gcgcgttccc tgctgacatg gcgatccgag tcctcatcgc cgatgaccac
    10321 gcggtcgtgg cagaggggct gcgcttcatc gtcgaggcgc aggcggacat ggaggtcatc
    10381 gcctgcgttc aggagagccg tgaggcggta cgccgcgcga tcgagatgcg cccggacgtc
    10441 gttctgatcg atcacgcgat gcccgtgctg aacggcgccg aagcaacaca cctcattcgc
    10501 gagcgctgtc cggagacccg cgtgatcatc ctgtcgatgt actccaatcg ggtgcatgtt
    10561 ctacgcgcac tgcaggccgg tgccaccgga tacgtggtca agaagtcggc cgcgaaggaa
    10621 gtcgtcgatg ccatcagggc ggttcacaag ggtgggcgct accttagcag ggaggtggcc
    10681 gacagcgtga tcgaccaggt tgtcggcaag gccgccaccg atgatccgat gaaacgcctc
    10741 agttcccgcg agcgtcaggt gttgcagatg ctcgtggagg gacatgccgt ggcggatatc
    10801 gccacgaccc tgtcgctttc acccaagacc gtggaaacct accgcgcccg gatgatggaa
    10861 aagctcgaaa tccacgactt cgccaccctg gtcaagttcg ccatccagca cggcatcacg
    10921 tcgctcgaat agcccgcatg tttcaccagt agccaacgca cgacggattg cgaaggctgg
    10981 agcggcgaat cggtgcaggg gcatgcggac tgcgtagttt ttgagcaaaa accccgcttg
    11041 gggcgacacg accttctgcc ccgttgtccg attctctgtt ttcggtttcc cggaggcgtt
    11101 cttttgtcag gagtttgtcg ggcttgcatc cgacaaactc ctcgcctgtc agggaaatcc
    11161 cgacgctgat tgtcggccac gcccgacagc gggcgtttcc cttcttggat agatttccgg
    11221 tcgttggcgg tgctccggca gaatgccggc tgcgcgacat cattacttga acgagtggag
    11281 cgaaatcgat gaacacggat tccccgcagg gacctattgc attggttgtg gaggataacg
    11341 acaccgtcag acgggccctg tgcgaccgga tccatacctc tttcgcccag ttccggctgc
    11401 gcgaggccgg caccgtggac gatgcgctga agatcgtcga gagcgaaaga gtggatctcg
    11461 ttctcatgga tttccggctg ccgggcagga acggggtcga cgggacgcgg atggtccttg
    11521 aacgctcgcc ccacacatcg gtggtcatgg tttcggtctt cgacgactca agccatcgtt
    11581 ccgctgcgag gcaggcgggc gcgagggcgt ttgtttccaa gcgcgcgatc ggcagggaac
    11641 tcatcccggt gatcgagagc ctggtgaatt gcgggcaata gccgagatgg cggcgcggga
    11701 caacatagag acgcagcagc atattctggt agtggacgac gatcggctgg tgctggctgc
    11761 gctggccgag ggattacgcg acgccggcta ccgtgtcacc ggtgcggtga gcggcgagga
    11821 cgcactcgaa atcgttgggc gtgacgcgcc caatctcgcg ttgctcgacg tgcgcatgcc
    11881 gggcatggac gggatcgaac tgggcaggcg gttgcgcgag caggccaacg tgccgttcct
    11941 ctacctcacc gcgtacggcg actgggacat cgtcaagcag gccgccgagc aaggcgcgct
    12001 cggctatctc gtcaagccgc tcgatatcca ccagatcgtg ccttcgatcg aggcggcgct
    12061 gaggcgcgcg cgcgagatca aaaaactgcg tgagagggaa gcgcagctta acgccgcgct
    12121 cgccggcacc cgcgagatca gcatggcggt gggcctgctc atgatgcgcg accgcctcga
    12181 ccgcgagcag gcattcgagt tgctgcgttt ccacgcccgt tcgcagcggc ggacggtcgc
    12241 ggaactcgcc agggagttgc tcagctcggc ggaaaatctc aatgttgtca gcaagttgaa
    12301 gcagaacgcc ccgcgcggaa agcgggggaa tacttgattc ccattactcg gacttgcgac
    12361 ctactgatca gagggatata aaaaacatgc agcggaaggc cgttaccccg ccgtccgcat
    12421 tctggtcgtt tcgcttaacc gcagtgcgcg ccgccccgtc cccgcagcgc gcctgaccta
    12481 ggcccagctc cccgcctgta agtgttggtg ttgcgtcgtc gcgcgtgtgt ggcgtgtgga
    12541 ggagtttccc gactatgtgg ggtgaacaac aagaacaaga agacaggcgc gatgcgcctc
    12601 gccgcaaatg cgggctatcg aggcgtgcga agctttgcaa gcgcggtctt cgttttagca
    12661 aggcgtcgcc agagctgacg cgcggcccgg aaaagggtcc tgaccccttt ttcctggttg
    12721 cggggatgcg cttatgcccg tgaccgacat gattcaccgg gcaacaaaca acagcgaatc
    12781 ggtgagacgg ttcatgtctc acgtccacac ccgctggggt tacgcgatcg tattctccct
    12841 gacgcttttg gcgatcatcg tcactgcggt gcaaagctac cgcgcgatcg accgcgagct
    12901 gaccgaagtg gcattgtcgc gcaggatagc ggttgcgcag ctcgccgccg ccacgttgtc
    12961 ggagaagctc gggcggctcg tggacgtcac tctttccctc gccacccgtg tccggttccg
    13021 ccagctcgtc gcctcagggg aatgggggga agcgatcaaa atcctgcgcg atgttccacg
    13081 cgactttccg tttaccgacc gcctggtcct tttcgatgcc cggggcacag tgatggcgga
    13141 cattccggaa cagctcgagg taagagggac gaattttgcc tatcgcgact ggtatcaagg
    13201 ggtcagccgc gactggaagc cctacgtctc cacagtttac agaagggcgg ccgcgccgca
    13261 gtccaacgta ttcgcggttg cagtcccggt tcgtagagat agcgacggtg aggtcgcagg
    13321 cattctgctg ttgcagacgc ggctcgacgc ctattttgaa tgggtgaggg atgccgaggt
    13381 cggccccagc gggtttgtct atgtcgtgga ccagaaagga caggttgcct ttcatccgaa
    13441 gtttccgccg caaggaacga ttgtcgacct tgcggccgtg ccggtggtgc agcgggtatt
    13501 gcgcggggat cgcggcatag aaatcgcgtt cgattcgacc gagaaggagg acgccatctc
    13561 ggcctatgcg ccggtcgcga agtacggctg gggggtcatc gtgcggcagc caaaacacgc
    13621 tgcctttgcg gccaaggatc agcagatcga acgcctgctg atcgcatacg gcctgacctt
    13681 tctgttcttc gtgctcggga tctatctggc ttcgcggatc gtcatccagc gcaggcaggc
    13741 cgaggaggac cgccggatga aggccgagct tgagcgaatg gtcgcggagc gcaccgccca
    13801 gctcgaagtg agcaacaagg agctcgaaag ctttagctac tccgtgtcgc acgacctgcg
    13861 ctcgccgctg cgcgcgatag acggcttttc gcgcatcctg caggaagaac acggtgacaa
    13921 gctgggcgat gaggcccggc gcctgcttgg catcatccgt gacaacagtc agaagatggg
    13981 ccgtttgatt gacgacctgt tgaagtactc gcgtcttggc cgcaagccgc tgtcaaccgc
    14041 tgagattgat atgaagcagt tggccgaaga agtattcggg gaattgcagg cgggaaacga
    14101 gcgaaggccc aagctgatgc tcggtgtgct gccgctggcg cacggcgatg cgacgttggt
    14161 gaagcaggca tgggtgaacc tgctggacaa tgcgatcaag ttcagcagca agcgtgaaca
    14221 accgatgatc gaggtgagcg gatacgggaa cagcgcggag agcgtgttct gcgtgaagga
    14281 caacggcgcc ggtttcgaca tgcgttacca cgacaagctg ttcggcgtgt tccagcgact
    14341 ccacgcggca gacgagtttc cggggaccgg agtcgggctc gccatcgtcc accgggtggt
    14401 tacccggcac ggcgggcgag tctgggctga aggtaaggtc ggcgaaggcg cggcattcta
    14461 ttttgcgttg ccggggaggg tccgggatga ctgagcctgt gcgtttgtcg cccccgttgc
    14521 ccgcaaaggc gccgggaaga ttcggccttc ccgcaagtct cgtcggcatt ctgctgccgt
    14581 ttgccgcctt cgcggtgcaa tggacgttct gggacgcaat cagcccttac gtctggttcc
    14641 ttttctatcc gacggttttc ttcgcaccgc tattcggcgg gctgtggagc ggcatcgtgg
    14701 cgacggtcat ttcgacgttg ctggtgtggt acgttttcat cccgacggca ttctccttca
    14761 ggatcgagga ccccatttcc atagtttcga tcgcgctgtt caccttgatg ggcgtcatct
    14821 tcagtctctt ccacgaacat ctgcgcaagc tcggtctgaa agtagcgcag caggaaagca
    14881 acgaacggtt caggagagct ctggacaaca tgatggaggg ttgccagatc attggtttcg
    14941 actggcgcta cctgtacgtc aatgcgaccg ccgccaaaca gggccgctcc accgtggcgg
    15001 agctgacggg acgcacgatg atggaagcgt acccgggcgt cgaaacaacg gcggcatttg
    15061 ccgcgctcaa acggtgcatg gaagccaggc ttccggaaca gatggaaaat gaattcgttt
    15121 atcctgacgg caccttggcc tggtttcagc ttgccattca gccggtgccg gaaggggtct
    15181 ttgtcgtctc gcttgacatc accaggcgca agcgggcgga ggaagaaatc cgtacgctca
    15241 acgccgagct ggaggagcgg gtggccgccc gcactgctca actggagttc gccaacaggg
    15301 agcttgaagg gtttgcctat tcggtttctc acgacatgcg cacgccgttg cgggcgattg
    15361 acgggttctc ccgcatcttc caggaggact atgccggcaa gctggacgat gaagcccggc
    15421 gcctcctcgg cgtcatccgt gacagcagcc ggaagatggg ccagttgatc gatgacttgc
    15481 tggagtactc gcgcctcagc cgcaagaccc tctcgacctc cgggatcaat atgacgcgca
    15541 tggtcgagga ggcgctcgaa gaattgcgga ctgccggggg gcggtggccc gaactggtgc
    15601 tcggggcttt gccgccagcg tgcggcgacg cggcgctggt caagcaggtg tggctgaatc
    15661 tccttgccaa cgccatcaag ttcagcagca agcgcgagcg gccggtgatc gaggtcagtg
    15721 gccgcgagga cgatgcggag agcgtgtact gcgtgaagga caacggcatc ggcttcgaca
    15781 tgcagtatta cgacaagctg ttcggcgttt tccagcggct ccacggcgtg gaggagttcg
    15841 agggaacggg ggtggggctc gccatcgccc agcgcgtgat cgcccggcac ggcgggcgcg
    15901 tctgggccga tggcaaggta ggtgaggggg cggcgttctg tttttctctg ccgagaggag
    15961 cacagcatgg ataaattcga gaaagtggac gttctgctga ttgaggacaa cccgaccgac
    16021 gcggaactga cgatacgggc gctgcgcaag ggcaggctgg caaacaacgt cacctgggtc
    16081 aaggacggcg cggaggcgct ggagttcatc tttcgcaatg gcgcctacgc cggtcgtccc
    16141 gacgacaacc cgaggctgat cctgctcgat ctcaagctgc cgaaggtgga tggtatcgag
    16201 gtgttgaagc gggtcaaggc ggacgagcgc acgcgggcca ttccggttgt tatggttacg
    16261 tcgtcgcagg aggggcgcga catcatcgag agctacaagc tcggcgtcaa cagctacgtc
    16321 gtcaagccgg tggaattcga gcaattcagc gaaaccgtgg ccaaggcagg tttttactgg
    16381 atgcttgtga acaaagcgcc gtaggcgggg ctagtcggag aaggggaagg ccggatggcg
    16441 aaggaactgc gtatcctgat gatcgaggac gtgccggcgg acgcggagct cgaaatccgc
    16501 gagctcaagc gcgccggctt gcgcctcgcg catcgtctcg tcgagaccga agcggcattt
    16561 cgcgatgcgc tgcgggattt cccgccggac gtgatcatct cggacttctc catgccgcat
    16621 ttcgacggca tgtgggcgct cgagcttgcg cgcgtgctcg ctccggacgt cccgttcatt
    16681 ttcgtctcgg gcacgatcgg cgaggagtac gcgatccgcg cgctgaagag cggcgcgacc
    16741 gactacgtgc tgaaggacaa tctggtgcgg ctgccgccgg cggtggagcg cgccctgcag
    16801 gacagcgcgg agcgcgccgc gcggcgcaag acggagcgcg agctggagga gacgcgcagc
    16861 cggctcgaca gtattgtcac ttcgctaagt gacgtcgtct ggtcgttttc cttgcgcgag
    16921 aatcgcatgc tctacgtgag ttccgcagcc aggaacatgt accgccagcc cgtcaatacc
    16981 ttctacgccg agcccatggc gtggatggac gcaatccatc ctgaagaccg cgagcgcgtc
    17041 atgcaggagt ggaacgcgtc gattgcggcg cgccgtttcg actccaccta ccgcatcgtt
    17101 cttcctgacg gggaggagct gtggctccac aatcgcggca acctcatcac ggatgaacag
    17161 gacgcgccgg tgcgtatcga cgggctggcg cgcgatatca ccgagcgcaa gcgagatgaa
    17221 gaagctttga gagaaagcga gtcgcggctc gatctcgcgc tgcaggcttc cggtctcgcg
    17281 acctgggagt ggaatcttac cactggagaa gtgcgcttca gccggcactg gtggccgatc
    17341 ctgggttacg gatctgatga ggttcccctg cgcgtcgagg cctgggagaa gctcacgcac
    17401 ccggacgacc tccagaaggt gaagtcgatg ctggcatccg ccgtgaaggg atcggatccg
    17461 gtactcgaca tcgagtaccg gatgcgcgcg aagagcggcg aatggtgctg gatccggacg
    17521 gtgggccagg tagtcgaacg cgatgcgacc ggacggacgt cgcggatgac tggcacgcac
    17581 ggtgacgtca ccgaacgcaa gcgggcggag gagaagatca ggcgcctcaa tcgggtgtac
    17641 gcggtgttga gcggcatcaa ttccgcgatc gtgcgcatcc gcgaccggca ggagctgttc
    17701 gaggaggcct gccgcatcgc tgtagaaggc gggaatttcg gcatggcctg gatcggcact
    17761 ctcgatccgg cggcgcagga agtgactcca gtggcgtggg cagggctcgg agccggcgat
    17821 tatctaggcg gcatcaagtc ccggatcagg gacgatcttc cgaccggtcg gggcgttctg
    17881 gcgcaggcgc tcaagacacg aaaacccgtc ttcgataacg acatcacgat caatcccgag
    17941 atgggcggag cgcggagaca ggaagcaata agacggggct accggtc
//
LOCUS       DBNGWZ010000414         9825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1938163, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000414 DBNGWZ010000000
VERSION     DBNGWZ010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9825
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1938163"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1270
                     /locus_tag="AC16CX_12465"
     CDS             275..1270
                     /locus_tag="AC16CX_12465"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013360.1"
                     /translation="MMIHPRLPPAILLSVALCTGMPDAVAQTKSGRSGSGFPTKPLRI
                     IVPYSPGGGLDFLARLVSQPLNERWGVPVIVDNRPGASGMVGNQIVAKAAPDGYTLAM
                     NSVEFITAPLVYRKSPYDTIKDFTGVVQTASQSYLLVVHPSVPAQSVRELVALSKTRP
                     LNYTSSGLGGMGHLSGELLKRMTGIEMLYVNYKGTGPALTDTLAGQVHIMFVNPLPAV
                     PHIKSGRLRLLASTDARRIALMPDVPTIAESGVPGFSTNGWNGMLAPAGTPRSVIRAI
                     NEAVAAIIKSPYGTERILAGGAEPAGGTPEQLNALMRDDHKRWSEIISTMKIGAE"
     gene            1377..2813
                     /locus_tag="AC16CX_12470"
     CDS             1377..2813
                     /locus_tag="AC16CX_12470"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3013361.1"
                     /translation="MANPDLRDWLAKIEAAGALQRVSGAERMEEIGGIVDIYQRQTGR
                     PTLLFDDIPGYPRGYRVAANILMGVKQIALTFGLPADSTEMDLVNYWRKYLGESKAIP
                     PVTVETGPVFENVATGQDINLAKIPTPKWHEDDGGPYIGTGCMVIMKDPDSGWINYGA
                     YRVQVYDDPRIASVMISKGKHGDILMRRYHERGERCPIAVVAGVHPMLFSIAGIEIPY
                     GKNEYDAVGGMMGNPVEVVLGPQTGLPIPANAEIAFEGYISPDDRIEEGPFGEWTGYY
                     AGGKEMQPVIRIETFMHRNDPILLGAIPAVPPCDDTFYRGTYRCAAVWRQLEGAGVPG
                     VQGVWAHEAGGSRMWLIVSINQMYPGHAKQAGLVASQCHAGAYANKWTIVVDDDIDPA
                     SINDVIWSLCTRVDAREDLEILRGCWSTTLDPTSYPEEERRLNSRVVIDACKPWTRRN
                     TFPRIARSSRQLDERIRAKWAHVLPKGG"
     gene            2816..3793
                     /locus_tag="AC16CX_12475"
     CDS             2816..3793
                     /locus_tag="AC16CX_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013362.1"
                     /translation="MKTTRTITSTLLILASLAFASAAGAQQIYPNKAIRVVVAFAPGG
                     ISDFVARSVSQKLSEVLAVPVVVENRAGAGGIVGAEAVAKSPPDGYTLLVTSISHTIN
                     PSVRKNLPFDAVRDFAPVTLLADAPNLLVVHSSLPVKSVKDVVALARSRPGQVTYASA
                     GAGTSTHLSGELFKAMTRIDIVHVPYKGGGPAVIDLIGGHVLMMFATLPSVLQHVRAG
                     KLRGLAVTGDRRFPAAQEFPTMIEAGIPGYEVSGWSGMFAPAGTPKDAVGRVAGEISK
                     ILRAPEMKERFFAQGAEPVGNTPEEFAAFVRSEIAKWRKVVEFSGMRAD"
     gene            complement(4012..4971)
                     /locus_tag="AC16CX_12480"
     CDS             complement(4012..4971)
                     /locus_tag="AC16CX_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013363.1"
                     /translation="MRRSDFDITDTIRTTDPVEVGSEVMRLYLGLYSGGTSPQIERAF
                     EDMVQLYRGEHPDYHPCDTPYHDIQHVLDVTLTTARLLDGYERGRQDGAPLPPEYFGI
                     GVVTALFHDVGYLRRRSDHKHRYGAEYTLTHVSRGSEFLLGYFPKLGWTQYAAAASEL
                     VHFTGYERPVDTIRLDDPVLHRVGEILGTADIIAQMSDRCYLEKCRDRLYPEFVLGGL
                     ARRKQPDGQVVTVYSSGSDLVHKTPGFYFNAMKRLDEQLHRAYTYAERHFGGRNAYLE
                     EMDKNIRYAQVMATGGGRDTLRRMMPITLPHLVRAYPEDLVIR"
     gene            complement(5114..6127)
                     /locus_tag="AC16CX_12485"
     CDS             complement(5114..6127)
                     /locus_tag="AC16CX_12485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3013364.1"
                     /translation="MKVYRLDPLGTLDGLTLGEEEDRRPGPGEVAIRVRATSLNYRDL
                     KVATGAYERGAIKPRVIPLSDGAGELVEAGPGVTRVKVGDRVAAIFVQRWLAGRVDPA
                     YASSALGGPSDGVLSERVVLPEEGIVLVPAHLNFEEAATLPCAAVTAWHALFARGGLT
                     AGETVLTLGTGGVSLFAAQFARMAGARVIVTSGSEEKIARLRAMGITDVVNYRTTPDW
                     ERAVMDLTGGQGADHVVELGGAGTFRKSLLALRVGGRLYAIGNLAGEAQINPQMILAK
                     RANVHGIQVGSREMFEAMNRAISQAKLKPVIDRQFEFGDARAAYDYLAGRAHFGKIVI
                     RGA"
     gene            6407..7846
                     /locus_tag="AC16CX_12490"
     CDS             6407..7846
                     /locus_tag="AC16CX_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013365.1"
                     /translation="MSVHKIDRRTVLKTAAAFAATGLGSCLPLVQTGPGGRSAGPLPG
                     RGEFVIRGATVLTLDAGIGDFARGDVHVRDGAIVAVAAEIVVATAPVIDGRGMICMPG
                     FIDTHWHLWTSVLRPLMRIDDPKLGYFPVSNRLGRHYTPQDSYRSVRLGLSEALSAGA
                     TTVHNWAHNVRTPGHADAELRAMRDMGMRGRFSYGPAQGMPDDQPMDLDGLARIKREW
                     MPNDGTLTLGICSRNVGKSQTTLRGTISLDIVRKDWGGARALGLPITLHTSGPSPIKL
                     LDDANLLGPDVQLVHPLLTTAEDRRILRERGVSYSTSPVGESRRPASAGVIQLAELME
                     AGVKVSLSIDHTGTFNCDFFEAMRILYNLHQHRVGARVPLTAKRLVQLATLDGAVDLG
                     IAGRTGSLTPGKRADIILVRTNDINIAPVGDPYEALVSFAQPRNVDTVIADGRILRRA
                     GEFTALDHAGVLKEAAESAAALVARANSS"
     gene            complement(7915..8544)
                     /locus_tag="AC16CX_12495"
     CDS             complement(7915..8544)
                     /locus_tag="AC16CX_12495"
                     /inference="COORDINATES: protein motif:HMM:NF015858.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HIR3013366.1"
                     /translation="MDGIIQKLSSAATLVFVIFLGGCAFGTRQATLIYPPAPEEGQLP
                     IARAAAKPNSKNIQIAIVQFNDQRSDKNVVGTVRNTFGMKTADVVAVNSVTEWVSKAL
                     ATELQNSGYTVRSGAPGDSSATAGAVVSGDILNIFCDMYMTYTGQVSLVARVSRGGKE
                     LFSNHYAGEGSAGVAWAATAESFAQSLAIALSSAVKKLVSDLDARLTAQ"
     gene            complement(8757..9611)
                     /locus_tag="AC16CX_12500"
     CDS             complement(8757..9611)
                     /locus_tag="AC16CX_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013367.1"
                     /translation="MRFIPLLALFLAPFLATLAIDPGVAKGSLQVGAETIPLKFAAAQ
                     MHDNAEKLLDRPRELRIVLADREIPQDALSGIAFPPVVQMAREGKVKGLFLRLDPGNR
                     RRVSVTVLRPMPSAQISLLNQTLTASRDAFKALDVGGNRVTGEIEYRDTSSTPSDYPK
                     IGYAVRFGAPLFNEPAVTADLRGKEAYDSPQVRVLRERARALAKGDIAIARQFSTERA
                     NRTADAFLARAGSQAPVYVREAAADLERSLKKLQRVVVRGDRAVVIFSGKEWMNLVRA
                     GGQWLLDD"
BASE COUNT         1702 a         3240 c         3119 g         1764 t
ORIGIN      
        1 catctccgcg tcccaatggg cgccgctcac cttgagcagc tcgcccatct tgtccacctg
       61 ctcgatccat ccccgcaggt cgccgtagtt gcccaccgtt ttggagacgg gcgctccgct
      121 gtcgccggcc atgaatgctc tcccggattt tgtcggaccg ggatccggca aaatcccgat
      181 gtaattcggt tacaatcccg ggaaactcta cagcagcgtc tggtggcgat caacccgcca
      241 tgcgtgcaac gccgggtgca cggaggagga caaaatgatg attcacccgc ggctgccgcc
      301 ggccattctt ctttccgtcg cactgtgcac ggggatgccc gatgcggtgg cgcaaacgaa
      361 atccgggcgg tccggctccg gcttcccgac gaagccgctg cgcatcatcg tcccgtactc
      421 gcccggcggc gggctcgatt tcctggcgcg cctggtctcg cagccgctga acgagcgctg
      481 gggcgtgccg gtgatcgtgg acaatcgtcc gggcgcgagc ggcatggtcg gcaaccagat
      541 cgtggcgaag gccgcgccgg acggttacac gctggcgatg aactccgtcg aattcatcac
      601 cgcgcccctg gtctaccgga aatctccgta cgacaccatc aaggacttca ccggggtcgt
      661 ccagaccgcc tcgcaatcct acctgctggt cgtccacccc tcggtgcccg cacagtcggt
      721 gcgcgagctg gtcgcgctca gcaagacgcg ccccctcaac tatacctcct ccggcctcgg
      781 cggcatggga catctgtccg gcgagctgct gaaacgcatg accggcatcg agatgctcta
      841 cgtgaactac aagggaaccg gtcccgcgct caccgatacc ctcgccggcc aggtgcacat
      901 catgttcgtc aatcccctgc ctgcggtgcc gcacatcaag tccggaaggt tgcggctgct
      961 cgcgagcacc gatgccaggc gcatcgcctt gatgccggac gtgccgacga tcgccgagtc
     1021 cggcgttccc ggcttctcga cgaacggctg gaacggcatg ctcgcgccgg ccggtacgcc
     1081 caggagcgtc attcgcgcga tcaacgaggc cgtggcggcg atcatcaagt cgccatacgg
     1141 cacggaacgc atcctggccg ggggagccga accggccggc ggcacgccag aacaactgaa
     1201 tgcgctcatg cgggacgacc acaagcgctg gtcggagatc atcagcacca tgaaaatcgg
     1261 ggccgagtaa aacgagctat cggccatcag ccttcagcta tcagctagga ccgttcttct
     1321 ggttgtaagc tgacggctga gagctgacag ctgagagctt gcgaaaggag caggcagtgg
     1381 caaatccaga ccttcgagac tggctcgcga aaatagaagc cgcgggcgcg ctgcagcgcg
     1441 tcagcggcgc cgaacgcatg gaggaaatcg gcggcatcgt cgatatctac cagcggcaga
     1501 cggggcgccc cacgctgctg ttcgacgaca ttccgggcta tccccgcggc taccgggtgg
     1561 cggcgaatat cctgatgggt gtcaagcaaa tcgccctgac gttcggcttg cccgccgatt
     1621 ccaccgagat ggacctcgtc aactactggc gcaagtacct gggcgaatcg aaggccattc
     1681 cccccgtaac ggtcgaaacc ggtccggtat tcgagaacgt cgccaccggc caagacatca
     1741 atctggcgaa gatccccact ccgaaatggc acgaggacga tggtggcccg tacatcggca
     1801 ccggctgcat ggtcatcatg aaggatccgg attcgggctg gatcaattac ggcgcctacc
     1861 gcgtgcaggt ctacgacgat ccgcgcatcg catccgtgat gatttccaag ggcaagcacg
     1921 gcgacatcct gatgcgtcgc taccacgagc gcggcgagcg ctgcccgatc gcggtcgtcg
     1981 ccggggtgca cccgatgctg ttctcgatcg ccggtatcga gattccttac ggcaagaacg
     2041 aatacgacgc cgtcggcggg atgatgggaa atccggtcga agtcgtgctg ggtccgcaga
     2101 ccggtttgcc gattcccgcc aacgccgaga tcgccttcga aggctacatc tcgcccgacg
     2161 accgcattga ggaaggcccg ttcggggaat ggaccggcta ctacgccggc ggcaaggaga
     2221 tgcagccggt catccggatc gagaccttca tgcaccgcaa cgatccgatc ctgctcggcg
     2281 ccatcccggc ggtgccgccg tgcgacgaca ccttctaccg cggcacctat cgctgcgccg
     2341 ccgtctggcg ccagctcgag ggcgcgggcg ttcccggcgt gcagggcgta tgggcgcacg
     2401 aggcgggcgg cagccgcatg tggctgatcg tttccatcaa tcagatgtac cccggccacg
     2461 cgaagcaggc ggggctggtc gcctctcagt gccacgccgg cgcctatgcc aacaagtgga
     2521 cgatcgtggt ggacgacgac atcgacccgg ccagcatcaa cgacgtgatc tggtcattgt
     2581 gcacgcgggt cgatgcgcgc gaggacctcg agatcttgcg cggctgctgg agcaccacgc
     2641 tcgatccgac gagctatccg gaagaggagc gccggctgaa ttcccgggtt gtcatcgacg
     2701 cctgcaagcc gtggacgcgg cgcaacacgt tccccaggat cgcccgctcg agccggcagc
     2761 tcgacgagcg catccgggcg aagtgggcgc acgtgctgcc caagggaggg taacgatgaa
     2821 aacaacgcgc acgattacga gcactctact gatactggcg agtctggcct ttgcgagcgc
     2881 tgcgggtgca cagcaaattt accccaataa ggcgatccgt gttgtcgttg cgtttgcgcc
     2941 tggcggcatc tcggacttcg tcgcgcgcag cgtgtcgcaa aagctgtcgg aggtcctcgc
     3001 cgtgccagtc gtcgtcgaga accgtgcggg tgcgggcggg attgttggcg ccgaggccgt
     3061 cgccaagtcg ccgccggacg gctacacgct gctggtgacg agcatctcgc acacgatcaa
     3121 ccccagcgtc agaaagaatc ttccattcga cgccgtgcgc gacttcgcgc cggtcaccct
     3181 gttagccgat gctcctaatc tgcttgtagt ccattcgtcc ctgccggtga aatccgtcaa
     3241 ggatgtcgtc gcgctcgccc gcagccgccc cggccaagtt acctacgcct cagccggcgc
     3301 cggcacttca acgcacctgt cgggcgagct gttcaaggcc atgacgcgga tagacatcgt
     3361 ccacgtgccc tacaaaggcg gcggtcccgc cgtcatcgac ttgatcggcg ggcacgtgct
     3421 gatgatgttc gctacgctac cctcggtgct gcaacacgtc cgagctggga agttgcgcgg
     3481 gcttgcggtg accggcgacc ggcgcttccc ggcggcccag gaattcccga ccatgatcga
     3541 ggccggcatt ccgggttacg aggtcagcgg ctggagcggc atgttcgcgc ctgccggcac
     3601 gcccaaagat gccgtcggcc gggtcgccgg cgagatttcg aagatcctgc gcgcgcccga
     3661 gatgaaggag cgattcttcg cccagggcgc cgagccggtc ggcaacacgc ccgaggaatt
     3721 cgctgccttc gtgcgttccg agatcgcgaa gtggaggaaa gtcgtggaat tcagcgggat
     3781 gcgggcggat tgatattttg tcgccttatc ggccgacaaa ttccccacat gtagtcccct
     3841 gccggggcct ggcgtcatcc cgctgatcgc gcctttcatc tgatagaggc tgagattcag
     3901 gccgactgat ttcatctgcc ggaaatgggg gtctgagctg ctccggtttt cttgtgcctt
     3961 ttcccggggc tgacccccga cacgccgcgg cctggaaggt gtgcgcaccg ctcagcggat
     4021 gaccaggtcc tccgggtacg cacgcacgag gtgcggcagc gtgatgggca tcatgcggcg
     4081 cagcgtatcg cgcccgccgc cggtcgccat cacctgcgcg tacctgatgt tcttgtccat
     4141 ctcctcgagg tacgcgttcc ggccgccgaa gtgcctttcc gcgtacgtgt aggcgcggtg
     4201 cagctgctcg tcgagccgct tcatcgcgtt gaaatagaac ccgggcgtct tgtgcaccag
     4261 gtcggagccg gatgagtaca cggtgacgac ctggccatcg ggctgcttgc ggcgcgcgag
     4321 cccgcccagc acgaactcgg ggtacaggcg gtcgcggcac ttctccagat agcagcgatc
     4381 ggacatctgc gcgatgatgt cggcggtgcc gaggatctcg ccgacgcggt gcagcaccgg
     4441 gtcgtccagg cggatggtat caaccgggcg ctcgtaaccg gtgaagtgca ccagctccga
     4501 ggccgccgcg gcgtactgcg tccagcccag tttcgggaaa tagccgagca ggaattccga
     4561 tccgcgcgac acgtgcgtga gcgtgtattc ggcgccgtag cggtgcttgt ggtcgctgcg
     4621 ccgccgcagg tagccgacat cgtggaacag cgcggtgacg acgccgatgc cgaagtactc
     4681 cggcggcagc ggcgccccgt cctggcggcc gcgctcgtaa ccgtcgagca ggcgcgccgt
     4741 cgtcagcgtc acgtcgagta cgtgctggat gtcgtggtac ggggtgtcgc aggggtggta
     4801 atcggggtgc tcgccgcgat agagctgcac catatcctcg aacgcgcgct cgatctgggg
     4861 cgacgtcccg cctgagtaca ggccgaggta gaggcgcatg acctcgcttc cgacctcgac
     4921 ggggtcggtg gtgcggatcg tgtcggtgat atcgaaatcg ctacgtcgca tcccgggaat
     4981 tttaaccccg ccgccatcaa tgagggctgt ctcttcttag gaacggcgtg attcgtgaat
     5041 cgtgattcgg ctcccgcagt gcgagagtca tccttcatcc ttctgcctta gctcctcgtc
     5101 acccgttact cggtcaggcg ccgcggatga cgatcttgcc gaagtgcgcg cggccggcga
     5161 gatagtcgta ggccgcccgg gcgtccccga actcgaactg ccggtcgatg accggcttga
     5221 gctttgcctg ggagatcgcc cggttcatgg cttcgaacat ctcgcggctg ccgacctgga
     5281 tgccgtgcac gttggcgcgc ttggcgagga tcatctgcgg gttgatctgc gcctcgcccg
     5341 ctagattgcc gatcgcgtag agccgcccgc ccacgcgtag cgcgaggagc gatttccgga
     5401 acgttcccgc gccgcccagc tcgaccacgt ggtcggcgcc ctgcccgccc gtcaggtcca
     5461 tgaccgcgcg ctcccagtcg ggcgtggtgc ggtagttcac cacgtcggtg atacccatcg
     5521 ccctgagccg cgcgatcttc tcttcgctgc ccgaggtgac gatcacgcgc gcccccgcca
     5581 tgcgcgcgaa ttgcgccgcg aacagcgaca caccgccggt gcccagcgtc agcacggtct
     5641 cgcccgcggt cagacccccg cgggcaaaca gggcgtgcca tgcggtgacg gctgcgcacg
     5701 gcagcgttgc cgcctcctcg aagttgaggt gcgcgggaac caatacgatg ccttcttccg
     5761 ggagcacgac gcgctcggac agcacgccat ccgacgggcc gccgagcgcg gaggaagcgt
     5821 aggccggatc gacgcgcccg gcgagccagc gctgcacgaa gatcgccgcg acgcggtcgc
     5881 ccaccttgac gcgcgtgacc ccggggccgg cttccacgag ttcgccggca ccgtccgaaa
     5941 gcggaatcac ccgcggcttg atcgcccccc gctcgtaggc gccggtggcg accttgaggt
     6001 cgcggtaatt gagcgaggtc gcgcggacgc ggatcgcaac ttcccccgga ccggggcggc
     6061 ggtcctcctc ctcgccaagc gtcaggccgt cgagggtgcc gagtgggtcg agtctgtaga
     6121 ctttcatgcg ctgttccttc ccctttgacc cgatcttaca atgcgaccct ggccgctggc
     6181 cgttctggtg cgggatttcg caaggggtct gaccccatgg atttcgttct cggcggccgc
     6241 aagggtcgag gccggagtgc tgctgcgtcg attgcagcgc ggcgagttca ttagaaattg
     6301 cagctttgtc gtgtcgcgtc aactggttgt ggtggtccga cccgggagtg ggaaaagctt
     6361 gccccttttc ccttagaatt ggccttcgca cagggggagg tccggcatga gcgtacacaa
     6421 gatcgatcgt cggacggttc tcaagacagc ggcggcgttc gcggcaacgg gcctcggatc
     6481 ctgcctgccg ctggtgcaaa cgggtccggg cggccggtcc gcggggccgc tgcccggtcg
     6541 cggcgaattc gtgatccgcg gcgccaccgt cctgaccctg gacgctggga tcggcgattt
     6601 cgcgcgcggc gacgtgcacg tgcgcgacgg cgcgatcgtg gcggtggcgg cggagatcgt
     6661 tgtggcaacc gcgcccgtca tcgacggccg cggcatgatc tgcatgcccg ggttcatcga
     6721 cacgcactgg cacctgtgga cgagcgtctt gcggccgctc atgcgcatcg acgatcccaa
     6781 gctcggctac tttccggtga gcaaccgcct cggccgccac tacacgccgc aggacagcta
     6841 tcgcagcgtc cggctcgggc tctccgaggc gctgagcgcc ggcgccacca cggttcacaa
     6901 ctgggcgcac aacgtccgca cgcccgggca cgccgacgcc gagctgcgcg ccatgcgcga
     6961 catgggtatg cgcggccgct tctcctacgg cccggcgcag ggcatgcccg acgaccagcc
     7021 gatggatctc gacggcctcg ccaggatcaa gcgcgagtgg atgcccaacg acggcacgct
     7081 tacgctgggc atctgctcgc gcaacgtcgg caagtcccag accaccctgc gcggcacaat
     7141 ctcgctcgac atcgtgcgca aggactgggg cggcgcacgc gcgctcgggc tgccgatcac
     7201 gctgcacacg tccggtccga gcccgatcaa gctgctcgat gacgcgaacc tgctggggcc
     7261 cgacgtgcag ctcgtgcatc cgctgctcac caccgcggag gatcgccgca tcctcaggga
     7321 gcgcggcgtg agctacagca cctcgccggt cggcgagtca cggcgcccgg cgagcgccgg
     7381 cgtcattcaa ctggcggagc tcatggaggc cggcgtaaag gtgagcctgt cgatcgacca
     7441 caccggaacc ttcaattgcg acttcttcga ggcgatgcgc atactctaca acctgcacca
     7501 gcatcgcgtc ggcgcgcgcg tgcccctgac ggcgaagcgg ctggtccagc tcgccacgct
     7561 ggacggcgcg gtcgatctcg gcatcgccgg ccggaccggc tcgctcacgc ccggcaagcg
     7621 ggcggacatc atcctggtgc gcacgaacga catcaacatc gcccccgtgg gcgacccgta
     7681 cgaggcgctg gtctcgttcg cacaaccgcg caacgtcgac accgtgatcg cggacggacg
     7741 gatcctgcgg cgcgcgggtg agttcaccgc gctcgaccac gccggggtgc tcaaggaggc
     7801 cgccgagtcc gccgccgccc tggttgcccg cgcgaactcg tcgtgatcct cgggcaaccg
     7861 gaaaaccggg gttacctcaa ggcccttttc aaggggtctg accccctgga ttccctattg
     7921 agccgtgagt ctcgcgtcca aatccgagac cagcttttta acggcggaag acaaggctat
     7981 tgccaaggat tgggcgaagg attccgcagt cgcagcccaa gccacccctg ccgacccttc
     8041 gcccgcgtaa tgattggaga acagctcttt tccacccctg ctgactcgtg cgaccagcga
     8101 aacttggccg gtgtaagtca tgtacatgtc gcaaaagatg ttcagaatgt ctccagacac
     8161 gacagcaccc gccgtcgctg aactgtcgcc gggcgctccg ctgcggaccg tgtaaccact
     8221 gttctgcagt tctgttgcca gggcctttga cacccactcc gtaacgctgt ttaccgcgac
     8281 gacgtcagcc gtcttcatgc cgaaggtgtt tctgacagta ccgacaacgt tcttgtccga
     8341 acgctggtca ttgaactgaa cgatggcaat ctggatgttc ttggaattcg gctttgccgc
     8401 cgcccgcgca atcggcaatt gaccctcctc aggtgccggc ggataaatca aagtcgcttg
     8461 ccgggttcca aaggcacaac cgccgaggaa aatgacaaag accagtgttg ctgcggagct
     8521 taacttttga atgattccgt ccatgttcga cattccatgg tttttatcac gcgtcccggt
     8581 attcggcaaa ttctactggt ttaaagagaa atcatcacac cagtctgaga cagtatctga
     8641 cacgcctttt tgacccatgt caaatgttca ttgactgcat agctgcaagc atggaaggat
     8701 ggctcccgcc gcgcgggaaa cctgtcactc gtcacctatc acgcttcttc ctgagttcag
     8761 tcatccaaaa gccattgccc gcctgcgcgc acaagattca tccactcctt cccggagaaa
     8821 atcaccacgg cgcggtcgcc gcgcaccact acgcgctgca gcttcttgag cgagcgctcg
     8881 aggtcggcgg ccgcctccct cacataaacc ggtgcctgtg agcccgcccg cgcgagaaag
     8941 gcgtcggccg tgcggtttgc gcgttcggtg gaaaattgcc ttgcaatggc gatatcgccc
     9001 ttcgccaggg cgcgggccct ttcgcgcagt acccgcactt gcgggctgtc gtacgcctct
     9061 ttgcccctga gatcggccgt gacggccggc tcgttgaaga gcggcgcgcc aaaccgaacc
     9121 gcgtagccga ttttcggata atccgacggg gtcgagctcg tatcgcgata ctcgatttcc
     9181 ccggttacgc gattgccgcc gacgtcaagc gccttgaacg cgtcccggct ggcggtgagc
     9241 gtctggttca ggagcgagat ctgcgcgctg ggcatcggcc tgagcacggt aacggatacg
     9301 cgcctgcgat tgccgggatc cagccgcaga aacaggcctt tcaccttgcc ctcgcgcgcc
     9361 atttgcacga ccggggggaa agcgattcca gatagcgcat cctgcgggat ctcgcgatcg
     9421 gcaagcacga tccgcaattc cctgggccgg tcgagtagct tctccgcgtt gtcgtgcatc
     9481 tgggcggccg cgaacttgag cgggatcgtc tcggcgccca cctggagcga gcctttcgcc
     9541 accccgggat cgatcgccaa cgtcgccagg aacggagcga ggaacagtgc cagcagcgga
     9601 atgaatcgca tggtcaatgg tcgtaaatgg tagtaaatgg gtggcctcct ttctgcgttg
     9661 gagtcatatt cgaggggcgc aatgtttgac gtacgctaat gcaagacctg acccccgcat
     9721 tgctcgttat gcgcgcagga cacgcgtctg gtcgttgaaa agttcgttga cctcgtactg
     9781 ccgggggatg agcccctgct gcgccgcgta gcggatgatc aactc
//
LOCUS       DBNGWZ010000415        14244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1182306, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000415 DBNGWZ010000000
VERSION     DBNGWZ010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14244
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1182306"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="AC16CX_12505"
     CDS             complement(<1..935)
                     /locus_tag="AC16CX_12505"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3013368.1"
                     /translation="MAELTYRDAVAAGIAQEMERDERVVFLGEDVAAAGGVFKATVGL
                     LEKFGPKRVRDCPISEQAIIGAAMGAAMTGLRPIAEIMFSDFLAVCWDMVANEIAKTR
                     YMTNGQISLPLVIRTANGAGSRFGAQHSQAVENWAMAVPGLKVVAPATPADVKGLIAA
                     AVRDPDPVIVFEQKSLYSMKGEVPDGEHVDQLGKAKVLRQGKDCTIVALALMVHRALD
                     AAEILEKEHGIACTVVDVRSLVPLDTRTILAEVAKTGRLVTVEENPRLCGWGAEIASI
                     VADECFWDLDGPIVRITTPHVPLPAADQLEDNAIPS"
     gene            complement(923..1948)
                     /locus_tag="AC16CX_12510"
     CDS             complement(923..1948)
                     /locus_tag="AC16CX_12510"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3013369.1"
                     /translation="MDRSAKRKKTTDSPGEIGRETRLELYRLQVELRYCEKRAYDLFL
                     QNLVKGTSHLSLGQEAIAAAFGVAMRPDDWTFCTYRGHAHTLARGASMAGVLGELMGR
                     ECGLLGGKGGSMHLTDVAKGAMGSYAIIGAHLPVAAGAAWSAQYRGTEQVAVCFFGDG
                     TVNIGAFHEALNFAAVWKLPVVYVCENNLYMEYTPIHTVTAVDHPAADRAAAYGLEPV
                     IVDGNDADEVYRAAVKHMNRARKGGGPALIEAKTYRHSGHSRADPAKYRPEGELEKWL
                     ERDPIKLYRERLTKLGVAEATLKDIEAESMRKVDEATAIAKASPVPSLDGIEKNVWAD
                     GGAAWRN"
     gene            complement(2075..3433)
                     /locus_tag="AC16CX_12515"
     CDS             complement(2075..3433)
                     /locus_tag="AC16CX_12515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3013370.1"
                     /translation="MQRKTTKPQARLVTRDSVQPKRRPKEAPDVLYDPANPGHRWERP
                     LQAPGRIQVDFEERIDFRRLHEYRLARTRQALAKSGLGALLVFDQYNIRYISSTVIGE
                     WARDKLTRWCLLTGNGEPWVWDFGSAARHHRLYAPWLPKNHWLAGLAGLRGAVSPRVG
                     LFEAAAKEIKDILVKEGVAKMPLGIDVVEPPFLFALQKLGLTVRDGQQVMLDAREIKS
                     HDEITLLNMAAAMGDGVYQDIYEALKPGVRENEIVAMATRRLYEMGSDCVEAINAIAG
                     ERCSPHPHNFTDRLIRPGDQAYFDIIQTYMGYKTCYYRTFTVGSATPPQHDAYRKARE
                     WMDRAIELLKPGVSTDEVAKAFPKCTEIGFETEMSAFGLNFCHGLGLGLHERPLISRV
                     NSFKDPYELKAGMVFAVETFCPATDGISAARIEEEVVLKPDGAQIITLFPAQELPIAN
                     RY"
     gene            complement(3464..4444)
                     /locus_tag="AC16CX_12520"
     CDS             complement(3464..4444)
                     /locus_tag="AC16CX_12520"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HIR3013371.1"
                     /translation="MLAAWYEKNGAAREVLRYGEQPDPLPGKGEVRVRLRASAVNPSD
                     VKARAGSRPVIPPRVIPDSDGAGEIDRVGAGVPRRRLRQRVWVYNGQWQRPFGTSAQY
                     IVLPSAQAVALPRKVSYAEGACLGIPVMTAHRCLFADGPVRGRNVLVTGGAGVVGHYA
                     VQLARWAGARVIASVSTEEKAAHAKQAGAHAVINYHSEDVASRLRELTGGEGIDRVVD
                     VEFGVNLPGYAKALHPDAVVATYASMRVREPQLPFYDLMRLNFTLRLVFVYTMLKSAK
                     TRALADIARWLAAGRPKFAIAARFPLNEIVAAHELVESGNKIGHVILDIP"
     gene            complement(4675..5772)
                     /locus_tag="AC16CX_12525"
     CDS             complement(4675..5772)
                     /locus_tag="AC16CX_12525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013372.1"
                     /translation="MSEVVERKATKDPRVEAAISHWAPRFVANGVPLTDFQEVTANCD
                     RWEDWCAAWSARAAVHEELGNKALAEGFKLSAGEHYTRAAVCYHFGKFLFVNDHEQMR
                     QAHRKAVECRNKALPLLDPPGERVAIPYEGKHLYGNLRKPRGVPKPPVVVMCMGLDST
                     KEEMDDYEKRFLKRGLATLAFDGPGQGEAEYDFAICPEYEKPVKAVIDFIETCKDLDA
                     GRVGIWGVSFGGYYAPRAAAFDKRIKACVALSGAYQRSASFEGRPVINVEAFRVRSKS
                     KDLEEAGKVALRVSLKGIAKNITCPIYIVAGTKDRLTPVEAARQLAAEVSGPCVLSIV
                     EGGNHVVNNLWYRYRDQTADWMAVQLGVCPA"
     gene            complement(5794..6762)
                     /locus_tag="AC16CX_12530"
     CDS             complement(5794..6762)
                     /locus_tag="AC16CX_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013373.1"
                     /translation="MYRLFVLSVAFTLSAAAWSADPLASYPSRPVRLITGSPGSTSDI
                     SARFIAQKLSERWGQQVVVDNRPGAGGIIGTEIGAAAVPDGYTLTVGHIGTHASAQFL
                     FKKLAYDPIKDFAPISLMTNSGIALAVHPSVPARDLKEFVALAKSKPGSLNYGSAGGG
                     TSSQLSGELFNQMTGARLVHIPYKGAGFALTALVAGETQAAFLSTTTIAVQAKAGKVR
                     PLAVLSPRRFPAAPDIPSAVEAGFPGLDSSVWFGLFAPAKTPRSIVVKINKEVVAALQ
                     LPEAKAALLSQGAEAVPTTPEEFDAFLKREIAKWGKVIKEAGLKAN"
     gene            complement(6773..7864)
                     /locus_tag="AC16CX_12535"
     CDS             complement(6773..7864)
                     /locus_tag="AC16CX_12535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3013374.1"
                     /translation="MDTAAKSQKDKRVEIAIHHWAPRFVSSDVPLADFEEVTAGISRW
                     DEWCAAWSARAAVHEELGRKALAEGFRLSAGEHLTRAAILYHFGKFMFVHDLEQMKRA
                     HRKVIECRNLALPHLEPPGERVLIPYEGAHLAGTLRKPAGVARPPVVVMCVGLDSTKE
                     EMDVYEDIFLARGMATLSFDGPGQGEAEYDIPIRGDYEVAVKAVVDYIETRSDLDPAR
                     IGLWGVSLGGYYSARAAAFEKRARACISLSGPYSWVETFDGRNELSREAFRVRSHSKT
                     MEEAREKAKTLTLEGVAGNITCPIFVVGGELDNLTPPHNAKRIASEVSGPCELLIVKG
                     GNHVANNRRYMYQTQTADWMAERLGAPGR"
     gene            complement(7975..8952)
                     /locus_tag="AC16CX_12540"
     CDS             complement(7975..8952)
                     /locus_tag="AC16CX_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013375.1"
                     /translation="MKTSTIRVCVVAIALAGGLAAPAAAQQKYPSKPIRMLVPFSAGS
                     QTDILARWIGEKVLESWGQQVVVDNRPSAGGTIASEYVLAANPDGHTLMMVSTGHAGN
                     ATLYSKLSYDTIRDFSGISRVASVPNLLVVAPTLGPKTMKELIAYAKGRPGQINFSSA
                     GIGSGTQINGEMFKLAAGIQATHVPYKGAPESLNETATGRVHFTFSPIVVAMGQAKAG
                     RVLVLAVSTAKRSPLFPDVPTVAEAGIPGFEYDQWYGLLASAKTPRAIIGQVNKEVVR
                     ILNLPDIKERMLTQGATPAPTTPEEFDAFIRSEVKRFAKVLIAAGARIN"
     gene            complement(8993..9961)
                     /locus_tag="AC16CX_12545"
     CDS             complement(8993..9961)
                     /locus_tag="AC16CX_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013376.1"
                     /translation="MIRKLGITLFTAVLAASGAAGAAEQYPTKPIRLMVPFPPGAASD
                     FLARVVGARMSEIYGQQAVIDNRPGAGGLIGSQIVAKAIPDGYTIALVGQPHLSNTLL
                     RDPHPYDPLKDFASVTLVAVIPNVIVLGNGVPATNVKELIALAKAKPGQLNFGSAGVG
                     SSSHLAGEMMVAAAGIKAVHVPFKTLGDIFSEMIAGRVHFYVFPLPAVMPMLKESRLK
                     AIATATPKRTPSLPNVPTTAEAGFGQYQSESWFGFVTPAGVPKWLIGKLNADFAKILQ
                     QPDIRERFQQGGAEASYGTPEQFLALQRDEYQRLAKLIKDLGIRVQ"
     gene            complement(9981..11000)
                     /locus_tag="AC16CX_12550"
     CDS             complement(9981..11000)
                     /locus_tag="AC16CX_12550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3013377.1"
                     /translation="MSNGVPLTDFEEVTAGLSRWEDWCRAWSARAAIHENLGRAALAD
                     GFRMSAGEHLTRAAVCYHFAKFVFVIDYEQMKQAHGKAVACRNLALPHLRPPGERVLI
                     PYEDKHLAGNLRKPAGAVRPPVVVMCMGLDSTKEEMESNESIFLARGMATLAFDGPGQ
                     GEGEYDFAIRGDYEAPVKAVLDFLEGRGDVDATRVGLWGVSLGGYYAPRAAAFEKRAK
                     ACIALSGPFDWIEFFDRLPELTRHTFRVRSKSRTDEEARSRAATLTLKDVAGNITCPL
                     FVVAGKQDRIVPWQEGERLAVAASGPVVRCFIEDGNHVANNRVYRYRAQSGDWMARQL
                     GAMAA"
     gene            11156..11752
                     /locus_tag="AC16CX_12555"
     CDS             11156..11752
                     /locus_tag="AC16CX_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR3013378.1"
                     /translation="MDVSFTSAVVILLLVMDPIGNIPLFVSLLRSAEPARRTRIILRE
                     CAIAFVVLLVFIFFGRPILGLLGLSDSSLTIAGGVILFLIALRMIFRRPEGVFGDVVE
                     GEPFIVPLAIPSIAGPTAIATVVLLVSRAPQRLLEWVAALTVAMAATLLLLVFAERIS
                     KLVGERGLLAFERLMGLILTAIAVEMLLRGIQTFVRGL"
     gene            complement(11870..12598)
                     /locus_tag="AC16CX_12560"
     CDS             complement(11870..12598)
                     /locus_tag="AC16CX_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013379.1"
                     /translation="MKTVVGPLLVVLALVPAYAVGADGVAIVLGEEIGGVELVQAGGG
                     PAQAARLLDLVWRRVARHYVERNGLAATAAEVAELAAYHREFDRKDRAQRARKIQQLN
                     QRLAADGLKAEERAWLEEFRAVLRRLERRDAENERSPPPDPAQQATLLTPWVEMWKMN
                     RALYEEYGGVVALTRFGPDPHGARRALIEDYERRGLLKFSDASLRERLFARLSAPPSM
                     IVAPGEVDFTPYWKRPIPPSYFPD"
     gene            complement(12943..>14244)
                     /locus_tag="AC16CX_12565"
     CDS             complement(12943..>14244)
                     /locus_tag="AC16CX_12565"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3013380.1"
                     /translation="LTQNIASIPFLWVVPLSLYLITFILCFDHPRWYHRGVFLALAAA
                     LLPAMAWYSDSLDLWTAAPLYAAGLFVCCMFCHGELTLLKPGPRYLTTFYLMVSVGGA
                     IGALLVGVAAPHLLSGYYELGIALTACAVLLYYRTLASRWWIAGASLAVVMATAAFTV
                     INISDYLANTRVKMRNFYGVIRTRDYPTPVPFRVMYHGGINHGGQLLDAEMRNRPTSY
                     FAPSSGYGRVFASLPDGPRRVGVIGLGAGAIAAYSRPGDLFRFYEIDPQVAAVAVTEF
                     TFLRDSPAQTDIILGDGRLSLEREPDQHYDLLAIDAFSGDSIPMHLITREAMASYVRH
                     LKPAGAIVFQATNRFVDIAPVVERLAAEFGLTAVLVSDVPESNEGADYWISGTDQIIV
                     TRNARLLASEPIRSVSEPLPTRPDFRVWTDDFYNLLRILKR"
BASE COUNT         2287 a         4784 c         4628 g         2545 t
ORIGIN      
        1 gacgggatcg cgttgtcctc cagctggtcg gccgcgggca gcggcacatg cggcgtggtg
       61 atgcgcacga tcggcccgtc gaggtcccag aagcactcgt cggcgacgat ggaagcaatt
      121 tcagcgcccc aaccgcagag ccgcgggttc tcttccaccg tcacgaggcg gccagtcttg
      181 gcgacttccg ccaggatagt ccgcgtgtcg agcggcacga gcgagcgcac gtccacgacc
      241 gtgcaagcga tgccgtgctc tttctccagg atctcggccg cgtccagcgc gcggtgcacc
      301 atgagagcga gcgcgacgat ggtgcagtcc ttgccctgtc gcagcacttt cgccttgccg
      361 agctggtcta cgtgttcgcc gtccggtacc tctcccttca tcgaatagag cgacttctgc
      421 tcgaacacga tcaccgggtc cgggtcgcgc accgcggccg cgatcaggcc cttgacgtcc
      481 gcgggcgtgg ccggcgccac cactttcagc cccggcaccg ccatcgccca gttctcgacc
      541 gcctgcgagt gctgcgcgcc gaagcgcgag cccgcgccgt tcgcggtgcg gatcacgagc
      601 ggcaggctga tctggccgtt ggtcatgtag cgcgtcttgg cgatctcgtt ggcgaccatg
      661 tcccagcata ccgcgaggaa atcggaaaac atgatctcgg cgatcggcct cagccccgtc
      721 atcgccgcgc ccatcgccgc gccgatgatc gcctgctccg agatcgggca gtcgcgcacg
      781 cgtttcggcc cgaatttctc gagcaggcca acggttgcct tgaacacgcc gccggcggcc
      841 gccacgtcct cgcccaggaa caccacgcgc tcatcgcgct ccatctcctg cgcgatgccg
      901 gccgcgaccg cgtcgcggta ggtcagttcc gccatgcggc gcctccgtcg gcccagacgt
      961 ttttctcgat gccgtccagc gacggaacag gagacgcctt ggcgattgcg gtcgcctcgt
     1021 ccaccttgcg catggactcg gcttcgatgt ccttcagcgt cgcctcggca acaccaagct
     1081 tcgtgagccg ctcgcggtag agcttgatcg ggtcgcgttc gagccacttc tccagctcgc
     1141 cctcggggcg gtatttcgcc gggtccgcgc gtgagtgccc gctgtggcga taggtcttgg
     1201 cttcgatcag ggcggggccg ccgcccttgc gcgcgcggtt catgtgtttc actgcggcgc
     1261 ggtacacctc gtcagcatcg ttgccatcta cgatgacggg ctccagcccg taggccgcgg
     1321 cgcgatccgc tgcggggtga tccacggccg tcacggtgtg gatcggggtg tattccatat
     1381 agaggttgtt ctcgcacacg tacaccaccg gcagcttcca cacggcggcg aaattgagcg
     1441 cctcgtggaa ggcgccgatg ttgacggtgc cgtcgccgaa gaagcagacc gcgacctgct
     1501 cggtgccgcg gtactgcgcg ctccaggcgg cccccgccgc gaccggaagg tgcgcgccga
     1561 tgatggcgta ggaacccatc gcgcccttgg cgacgtcggt gagatgcatc gagccgccct
     1621 tgccgccgag aagcccgcat tcgcggccca tcagctcgcc cagcacgccc gccatcgaag
     1681 cgccgcgcgc cagcgtgtgc gcgtggccgc ggtaggtgca gaaggtccag tcgtccggac
     1741 gcatcgcgac gccgaatgcg gcggcgatcg cctcctgacc gagcgacagg tggctcgtgc
     1801 ccttgaccag gttctgcagg aacaggtcgt aggcgcgctt ctcgcagtag cgcagctcga
     1861 cctgcaggcg gtagagctcg agccgcgtct cgcgcccgat ctctcctggg gaatcagtcg
     1921 tcttcttacg tttggcagat ctgtccattc tatggatccg gtgaggcgtt aggcgttagg
     1981 cgttaggcgt taggcgttag gcgttaggcg ttaggcgaac gacagcattg gtttgcagcg
     2041 gttctgtcgc ctcacgcctc acgcctcacg tatttcagta tctgttggca atgggcagct
     2101 cctgcgccgg gaacagcgtg atgatctgcg cgccgtcggg cttgagcacc acttcctcct
     2161 cgatgcgcgc ggccgagatg ccgtccgtcg ccgggcagaa ggtctccacc gcgaacacca
     2221 tccccgcctt cagctcgtac gggtccttga acgagttcac ccgcgagatg agcggccgct
     2281 cgtgcaatcc gagcccgagc ccgtgacaga aattgagccc gaacgcggac atctcggtct
     2341 cgaatccgat ctcggtgcac ttcgggaacg ccttcgccac ctcgtcggtg gagacgcccg
     2401 gcttgagcag ctcgatcgcg cggtccatcc actcgcgggc cttccggtag gcgtcgtgct
     2461 gcggcggggt cgcgctgccc accgtgaagg tgcggtagta gcaggtcttg tagcccatgt
     2521 aggtctggat gatgtcgaaa taggcctggt cgcccgggcg gatcagccgg tcggtgaaat
     2581 tgtgcgggtg cgggctgcag cgctcgccgg cgatggcgtt gatagcctcg acgcagtccg
     2641 agcccatctc gtagagccgc cgggtcgcca tcgcgacgat ctcgttctcg cgcacgccgg
     2701 gcttgagcgc ctcgtagatg tcctggtaga cgccgtcgcc catcgcggcg gccatgttga
     2761 gcagcgtgat ctcgtcgtgg ctcttgatct cgcgcgcgtc cagcatcacc tgctggccgt
     2821 cgcgcacggt cagcccgagc ttctgcagcg cgaacaggaa cggcggctcc acgacgtcga
     2881 tgccgagcgg catcttcgcc accccttcct tgaccaggat gtccttgatc tccttcgccg
     2941 cggcctcgaa caggccgacc cggggcgaaa ccgcgccgcg cagcccggcg agccccgcga
     3001 gccagtggtt cttcggcagc cacggcgcgt agagcctgtg atggcgcgcg gcggagccga
     3061 agtcccacac ccagggctcg ccgttgccgg tgagcaggca ccagcgcgtg agcttgtcgc
     3121 gcgcccactc gccgatcacc gtgctcgaga tgtagcggat gttgtactgg tcgaacacga
     3181 gcagcgcgcc gaggcccgat ttggcgagcg cctggcgcgt gcgcgcgaga cggtattcgt
     3241 gcaagcggcg gaaatcgatg cgttcctcga aatccacctg gatgcggccg ggcgcctgca
     3301 gcgggcgctc ccagcggtgc cccgggttgg ccggatcgta gagaacgtcc ggcgcctcct
     3361 tcgggcggcg cttgggttgg acgctgtccc gcgtgacgag acgcgcttgc ggtttggtgg
     3421 tcttgcgttg catggttatc tccggagacg ggcggatttg cgctcaggga atgtccagta
     3481 tgacgtggcc gatcttgttc cccgactcca ccagctcgtg agcggccacg atctcgttca
     3541 acgggaagcg cgcggcgatc gcgaacttcg ggcggcccgc agcaagccag cgcgcgatat
     3601 cggccagtgc gcgcgttttc gctgacttca gcatcgtgta tacgaaaacc aggcgcagcg
     3661 tgaagttgag ccgcatgaga tcatagaaag gaagctgggg ctcgcgcacc ctcatcgaag
     3721 cataggttgc gaccacggcg tccgggtgca acgccttcgc gtaaccgggc aggttgacgc
     3781 cgaactcgac gtcaacgaca cggtctatgc cctcgccgcc ggtcagctcg cgcagccggc
     3841 tcgcaacgtc ctcgctatgg taattgatca ccgcgtgcgc gcccgcttgc ttcgcgtgcg
     3901 cggccttttc ctcggtgctc acgctggcaa tgacgcgggc gcccgcccag cgcgcgagct
     3961 gcaccgcgta atgcccaacg acgccggcgc cgcccgtcac caagacgttg cgcccgcgca
     4021 ccggcccgtc ggcaaacagg cagcggtgcg cggtcatcac cgggatgccg agacaggccc
     4081 cttcggcgta ggaaacctta cgcggcaacg ctaccgcctg cgcggagggc agaacgatgt
     4141 attgcgccga cgttccgaac ggacgctgcc actgcccgtt gtatacccac acccgctggc
     4201 gcagccggcg tcgcggcacg ccggctccga cgcggtcgat ttcgccggcg ccgtcgctgt
     4261 ccggaatcac ccgcggcggg atcaccggac ggctgcctgc tcgcgccttg acgtcggagg
     4321 ggttcaccgc ggacgcgcgc aaccggacac gcacctcccc ttttcccggc aggggatcgg
     4381 gctgctcgcc gtagcgcagc acctcgcggg cggcgccgtt cttttcgtac cacgctgcaa
     4441 gcaccgtgtt cctccgatga cccgcctgaa gcgccctcac gtctttggcg cgctcagcag
     4501 cctgtcggag ctgaaagtcc gcacaagctg ctgacagcaa gaccgcccga catttcagaa
     4561 gaaaacgctt cgcaggcgga caaatatgaa caagagctga ttcgaattcg catatacgtt
     4621 atccctgtat tgcgtttttc cttgtgccgg tcttgcatca ggagtttgcc ggggctatgc
     4681 cgggcaaact cctagctgca ccgccatcca gtcggcggtc tggtcgcggt agcggtacca
     4741 cagattgttg acgacgtggt tgccgccttc gacgatcgac agcacgcacg ggcccgacac
     4801 ttccgccgca agctgcctcg ccgcttcgac cggggtcagg cggtccttgg tgccggcgac
     4861 gatgtagatc gggcaggtga tgttcttcgc gatgcccttc agcgacacgc gcagggccac
     4921 cttgcccgct tcctcgaggt ccttgctctt ggagcgcacg cggaacgctt ccacgttgat
     4981 gaccgggcgc ccctcgaagc tcgcggagcg ctggtaggcg ccggacagcg cgacgcacgc
     5041 cttgatgcgc ttgtcgaacg cggccgcgcg cggcgcatag tagccgccga aggacacgcc
     5101 ccagatgccg acgcggccgg cgtcgaggtc cttgcacgtc tcgatgaaat cgatgaccgc
     5161 cttcaccggc ttctcgtatt cggggcagat cgcgaaatcg tactcggcct cgccctgccc
     5221 cggcccgtcg aaggcgagcg tcgcgagccc gcgcttgagg aaccgcttct cgtagtcgtc
     5281 catctcctcc ttggtcgagt cgaggcccat gcacatgacg acgaccgggg gcttcggcac
     5341 gccgcgaggc ttgcgcaagt tgccgtagag atgcttacct tcgtagggaa tcgccacgcg
     5401 ttcaccggga ggatcgagca gcggcagcgc cttgttgcgg cattccacgg ccttcctgtg
     5461 cgcctgcctc atctgctcgt ggtcgttcac gaacaggaat ttgccgaagt ggtagcacac
     5521 ggcggcgcgc gtgtagtgct cgccggcgga gagcttgaat ccctccgcca gcgccttgtt
     5581 gccgagctcc tcgtggacgg cggcgcgcgc gctccacgcc gcgcaccagt cttcccagcg
     5641 gtcgcagttc gcggtcactt cctggaaatc cgtgagcggc acgccgttcg cgacgaagcg
     5701 cggcgcccag tgcgagatcg ccgcttccac ccgagggtct ttcgttgctt tcctttccac
     5761 cacttcagac atgttcacac tctcctgaat gagtcagttc gccttgaggc ccgcttcctt
     5821 gatcaccttg ccccacttcg cgatttcgcg cttgagaaaa gcatcgaact cctcgggcgt
     5881 ggtcggcacc gcctcggcgc cctgggacag caacgccgcc ttcgcttcgg gaagttgcag
     5941 cgccgccacg acttccttgt tgatcttcac cacgatcgac ctgggtgtct tggccggagc
     6001 gaaaaggcca aaccaaacgc tggagtcgag gccggggaag cccgcttcca ctgcactcgg
     6061 aatatccggg gccgccggga atcgcctggg gctgaggacg gcgagcggcc tgaccttgcc
     6121 ggccttcgcc tgcacggcga tcgtcgtcgt cgacaggaac gccgcctggg tttcgcccgc
     6181 caccagcgcg gtcagtgcaa atcccgcacc cttgtacggg atgtgcacca gcctggcgcc
     6241 ggtcatctgg ttgaacagct cgccggagag ctggctgctt gtgccgcccc cggccgagcc
     6301 gtaattcagg ctgccgggct ttgacttcgc caaggcgacg aattccttga ggtctctcgc
     6361 cgggaccgat ggatgcaccg ccagcgcgat ccccgagttc gtcatgagcg agatcggtgc
     6421 gaagtccttg atcgggtcgt aggccagctt cttgaacagg aattgggcgc tggcgtgcgt
     6481 gccgatgtgg ccgacggtaa gcgtgtagcc gtcgggcact gccgcagccc cgatctcggt
     6541 gccgatgatg ccgcccgcgc cggggcggtt gtcgaccacg acctgctggc cccagcgctc
     6601 ggagagcttc tgcgcgatga agcgcgccga gatatcggac gtgctgcccg gcgatccggt
     6661 gatgaggcgc accggccggc tcgggtagct tgccaggggg tcggcgctcc aggcggctgc
     6721 cgataaagta aaagccacgc tcaaaacaaa aagtctgtac atgccgtttt cctcagcgcc
     6781 cgggcgcgcc caggcgttcc gccatccagt ccgcggtctg ggtctggtac atgtagcggc
     6841 ggttgttggc gacgtgattg ccgcccttca cgatcaagag ctcgcatgga cccgaaacct
     6901 cggatgcgat gcgcttcgcg ttgtgcggcg gcgtgaggtt gtcgagctcg ccgccgacca
     6961 cgaagatcgg gcaggtgatg ttccccgcca cgccctcgag cgtcagcgtc ttcgccttct
     7021 cccgcgcttc ctccatggtc ttgctgtggg agcgcacgcg gaacgcctcg cgcgagagct
     7081 cgttgcggcc gtcgaaggtc tccacccagc tgtaggggcc ggagagcgag atgcaggccc
     7141 tggcgcgttt ctcgaacgca gccgcccgcg ctgagtagta gccgccaagc gagacgcccc
     7201 ataacccgat gcgcgcgggg tcgaggtcgg aacgagtctc gatgtaatcc acgaccgcct
     7261 ttaccgccac ttcgtaatcg ccgcggatcg ggatgtcgta ctccgcctcg ccctggcccg
     7321 gcccgtcgaa cgagagcgtc gccatgccgc gcgcaaggaa gatgtcctcg tagacgtcca
     7381 tctcctcctt tgtcgaatcg agcccgacgc acatcaccac gaccggcggc ctggcgacgc
     7441 ccgccggttt acgaagggtg cccgcgaggt gtgcgccctc ataggggatc agaacgcgct
     7501 cgccgggagg ctccagatgg ggcagcgcga gattgcggca ttcgatcacc ttgcggtgcg
     7561 cgcgcttcat ctgctccaga tcgtgcacga acatgaactt gccgaagtgg tagagaatcg
     7621 ccgcacgggt caggtgctcg cccgcggaca gcctgaaccc ctcggcgagc gcttttcggc
     7681 cgagctcctc gtgaacggcg gcgcgggcgc tccacgccgc gcaccactcg tcccagcgcg
     7741 agatgcccgc cgtcacctcc tcgaagtccg caagcggcac gtcgctcgag acgaagcgcg
     7801 gcgcccagtg atggatcgcg atctcgacgc gcttgtcctt ctggcttttt gccgccgtgt
     7861 ccactctcat tcagtccaag gaaaaaactt cgacgcaaag tcgcgaaggc gcaaaggaga
     7921 atcacaatta ttcatttctt tgcggctttg cggcttcgcg tccaatgcct tcacctagtt
     7981 gatccgcgcg ccggcggcga tcagcacctt ggcgaagcgc ttcacttcgg agcggatgaa
     8041 ggcgtcgaac tcctccggcg tggtgggtgc cggcgtcgcg ccctgcgtca gcatccgctc
     8101 cttgatgtcg ggcaggttga ggatgcgcac gacctccttg ttgacctgcc cgatgatggc
     8161 gcgcggcgtc ttggccgatg ccagcagccc gtaccactgg tcatattcga agcccggtat
     8221 gcccgcctcc gccaccgtgg gcacgtcggg aaacagcggc gagcgtttcg cggtgctgac
     8281 cgcgagcacg agcacccgcc ccgccttggc ctgccccatc gcgaccacga tcggcgaaaa
     8341 cgtgaaatgc acgcgcccgg tcgccgtctc gttgagcgat tcgggggcgc ccttgtaggg
     8401 aacgtgggtc gcctggatgc ccgcagcgag cttgaacatc tcgccgttga tctgggtgcc
     8461 gctgccgatg cccgcggagc tgaagttgat ctggccgggc cggcctttcg cgtaggcgat
     8521 cagctccttc atcgtcttcg gccccagcgt gggcgccacc accagcaggt tgggaacgct
     8581 tgccacgcgc gatatgccgg agaaatcccg gatggtgtcg taggacagct tcgagtagag
     8641 cgtcgcgttg ccggcgtggc cggtggacac catcatcagc gtatggccgt cgggattcgc
     8701 ggccagcacg tactccgacg cgatggtgcc gcccgcgctc gggcgattgt ccaccacgac
     8761 ctgctggccc cagctctcga gcaccttctc cccgatccag cgcgcgagga tgtcggtctg
     8821 gctgccggcc gagaacggca ccagcatccg gatcggcttc gacggatact tctgctgggc
     8881 cgcggccggg gccgcgagcc cgcccgccag cgcaatcgcc acgacgcata cgcggatcgt
     8941 cgatgtcttc atgtcccctc ccttttttag aacggtcccc cgcggcgccg gatcattgca
     9001 ccctgatgcc gaggtccttg atgagcttgg cgagccgctg gtactcgtcg cgctgcaggg
     9061 cgaggaactg ctccggcgtg ccgtagctcg cctccgcgcc gccctgctgg aagcgctcgc
     9121 gaatgtccgg ctgctggagg atcttcgcga aatcggcgtt gagcttgccg atcagccact
     9181 tcggcacgcc cgccggcgtc acgaatccga accacgattc cgactggtac tgcccgaatc
     9241 cggcctccgc cgtggtgggc acgttgggca gggacggcgt gcgcttcggc gtcgccgtcg
     9301 cgatcgcctt gaggcggctc tccttcagca tcggcatcac ggccggcagc gggaacacgt
     9361 agaaatgcac gcgcccggcg atcatctcgc tgaaaatgtc gccgagggtc ttgaacggca
     9421 cgtgcaccgc cttgatgccg gccgccgcca ccatcatctc gcccgcgaga tgcgaggagc
     9481 ttcccacgcc agccgagccg aagttgagct gccctggctt ggctttggcg agcgcgatga
     9541 gctccttgac gttggtcgcg ggcacgccgt tgccgagcac gatgacgttg gggatgacgg
     9601 cgaccagcgt gacggaagcg aaatccttga gcggatcgta cgggtgcggg tcgcgcagca
     9661 gcgtgttcga gaggtgcggc tggccgacga gggcaatcgt gtagccgtcg ggtatcgcct
     9721 tcgcgacgat ctggctgccg atcaacccgc cggcgccggg acggttgtcg atcaccgcct
     9781 gctggccgta gatctccgac atgcgggcgc ccaccacgcg cgccaggaaa tcgctcgccg
     9841 cccccggcgg gaacggcacc atgaggcgga tcggcttggt gggatattgt tccgcggccc
     9901 ctgccgcgcc ggacgccgcg agaaccgccg tgaaaagcgt gatgccgagc ttgcgtatca
     9961 tagcctcctc cttgttgttt ctaggcagcc atggccccca gctgccgcgc catccagtcg
    10021 ccggactgcg cgcgataccg gtaaacgcgg ttgttggcga cgtgattgcc gtcctcgatg
    10081 aagcaccgca ccacgggccc ggacgcggcc accgcgaggc gctcgccttc ctgccacggc
    10141 acgatgcggt cctgctttcc cgcgacgacg aacagcgggc acgtgatgtt cccggcgacg
    10201 tccttgaggg tcagcgtcgc ggcgcggctg cgggcctcct cgtcggtgcg gctcttggag
    10261 cgcacccgga acgtgtggcg cgtcagctcg ggcagccggt cgaagaattc gatccagtcg
    10321 aacggtcccg acagcgcgat gcacgccttc gcccgtttct cgaacgccgc cgcccgcggc
    10381 gcgtaatagc cccccagcga cacgccccac aggccgacgc gcgtggcatc cacgtcgccg
    10441 cggccctcga gaaagtcgag cacggccttt accggagcct cgtagtcgcc gcggatcgcg
    10501 aagtcgtact cgccttcgcc ctgtcccggc ccgtcgaaag cgagggtggc catgccgcgc
    10561 gcgaggaaga tgctttcatt ggattccatc tcctccttgg tcgagtcgag ccccatgcac
    10621 atcaccacga ccggcggtct gacagctccc gccggtttac gcaggttgcc cgcgagatgc
    10681 ttgtcctcgt aggggatcag cacccgctcg ccagggggtc gcagatgggg cagcgcgaga
    10741 ttgcggcaag cgaccgcctt tccgtgcgcc tgcttcatct gctcatagtc gatgacgaac
    10801 acgaacttgg cgaagtggta gcagaccgcg gcgcgcgtca ggtgctcgcc ggcggacatc
    10861 ctgaatccgt cggccagggc ggcgcggcca agattctcgt ggatcgccgc gcgcgcgctc
    10921 caggcgcggc accagtcctc ccagcgggaa aggccggcgg tcacctcctc aaagtcggtc
    10981 agcggaacgc cgttcgacac gaagcgcggc gcccaatgcg caatcgccga ctgtaccctt
    11041 gaatccggca ttgacgattt ttgactggga ttgctggaca gggaccaacc aattgccaat
    11101 ggtatcaata atcggtccgt tatgcgcgta aactggccat ttcaggcgtc tttccatgga
    11161 tgtctcgttc acctcggccg tcgtgatcct gctgctggtc atggacccga tcggcaacat
    11221 tccgctgttc gtgtcgctgc tgcggagcgc ggagccggcg cggcgcacgc ggatcatcct
    11281 gcgcgagtgc gcgatcgcgt tcgtcgtgct gctcgtcttc attttcttcg gtcggcccat
    11341 actcggactg ctggggcttt ccgattcctc gctgaccatc gccggcggcg tgatcctgtt
    11401 cctgatcgcc ctgcggatga tcttccgccg gccggagggc gtctttggcg acgtcgtgga
    11461 aggcgagccg ttcatcgtgc cgctcgctat cccctcgatc gccggcccga cggcgatcgc
    11521 gaccgtggtg ctgctggtgt cgcgcgcgcc gcaacgattg ctcgaatggg tcgcggcgct
    11581 cacggtcgcc atggcggcca cgctgctcct gctcgttttc gccgagcgca tcagcaagct
    11641 ggtgggcgag cgggggttgc ttgccttcga gcgcctgatg ggcctgatcc tcaccgcgat
    11701 cgcggtggaa atgttgctgc gcggcatcca gaccttcgtg cgcggcctgt gaacgcgagt
    11761 gactaagtga ctaggtaatc aggtgattaa gcaaaagcac cggctcacgc ggctacactc
    11821 acactcaatc acttagtcac tcagtcacct agtcactcct tcccgggcgt cagtcgggaa
    11881 agtaggaggg cggtatgggc cgcttccagt agggagtgaa atccacctcc ccgggagcga
    11941 ctatcatcga gggaggcgcc gacagccgcg cgaacagccg ctcccgcagg gaggcgtcgg
    12001 aaaatttcag caggccccgg cgctcgtaat cctcgatcag cgcccggcgc gcgccatggg
    12061 ggtcgggacc gaagcgggtg agcgcaacga caccgccata ttcctcgtag agcgcccggt
    12121 tcatcttcca catctccacc cacggcgtga gcagggttgc ctgctgcgcg gggtcgggtg
    12181 gcggcgaccg ttcgttctcg gcgtcgcgtc gctccaggcg ccggagaacg gcgcgaaatt
    12241 cctcgagcca ggcgcgttcc tcggctttca gtccatcggc agccaggcgc tggttcaact
    12301 gctggatctt gcgcgcacgc tgggcccggt ccttgcggtc gaattcgcgg tgataggccg
    12361 ccagctcggc cacctccgcg gcggtggcgg cgagcccgtt ccgctcgaca taatgcctgg
    12421 ccacccggcg ccacaccagg tcgaggagcc tggcggcttg ggccggcccg cctccagctt
    12481 gaacgagctc cacaccgccg atctcctcgc ccagcacaat tgccaccccg tccgcgccta
    12541 cggcataggc gggcaccagg gcgagcacaa cgagtaacgg gccaaccacg gtcttcatcc
    12601 ggcaaattct agcgcgacgc ggggcgaata cggtcccggg taacacccgg tccggtcatc
    12661 gcctcccggc ataggccgtt gatttatttg catgatgggc atggcagcgc tttgcatcgg
    12721 ctcgcgcgag aaataattca caatttttgt ggataaccat gtgggtaacc ggtaacgata
    12781 agcgcgaatg aggcgtaacg aggggctcgc gtctggtttg cacaaaaatt catcaaacag
    12841 ccgtaagcag taaacggtta gcggtgagcg gtcaagttga tggccgctcg cgttagccgt
    12901 tccgcgcttt cggctcactg cttactgctt accgcttgcg gctcaacgct tgaggatgcg
    12961 cagcaggttg tagaaatcgt cggtccaaac gcggaaatcg gggcgggtcg gcagcggttc
    13021 ggacaccgag cggatcggct cggaagccag cagccgcgcg ttcctcgtga cgatgatctg
    13081 atcggtgccg gaaatccagt aatcggcgcc ctcgttgctt tccggcacgt cggagaccag
    13141 caccgcggtc aggccgaact cggcggcaag gcgctccacc accggcgcga tgtccacgaa
    13201 ccggttggtc gcctggaaca cgatcgcgcc cgcgggtttg aggtggcgca cgtagctcgc
    13261 catcgcctcg cgcgtgatca gatgcatcgg tatcgagtct cccgaaaagg cgtcgatcgc
    13321 aaggaggtcg tagtgctggt cgggttcgcg ctcgagcgag agccgcccgt cgccgaggat
    13381 gatgtccgtc tgggccgggc tgtcgcgcag gaaggtgaac tccgtgaccg cgaccgcggc
    13441 cacctggggg tcgatctcgt agaagcggaa caggtcgccc ggccgcgagt aggcggcgat
    13501 cgcccctgct ccgaggccga tcacgccgac acgccgcggg ccgtccggca ggctcgcgaa
    13561 tacgcgcccg tagccgctcg acggcgcgaa gtagctggtg ggccggttgc gcatctccgc
    13621 gtcgaggagc tggccgccgt ggttgatgcc gccgtggtac atgacgcgga acgggaccgg
    13681 ggtcgggtaa tcccgggtgc ggatcacgcc gtagaaattg cgcattttca cacgggtgtt
    13741 ggccagatag tcgctgatat tgatcaccgt gaacgcggca gtcgccatca ccaccgcgag
    13801 cgaggcgccg gcgatccacc agcggctcgc gagcgtgcgg taataaagca gcaccgcgca
    13861 cgcggtgagc gcgatcccca gttcgtaata gcccgagagc agatgggggg ccgcgacacc
    13921 caccagcagc gcgccgatcg cgccccccac cgacaccatc agatagaacg tggtcagata
    13981 gcgcgggccg ggcttgagca gggtcagctc gccgtggcag aacatgcaac acacgaacaa
    14041 tcccgccgcg tagagcggcg cggcggtcca cagatcgaga gaatcgctgt accaggccat
    14101 tgccggcagc agcgccgccg ccagcgcgag gaacacccca cggtgatacc agcgcggatg
    14161 gtcgaagcac aggatgaagg tgatgagata gagcgaaagc ggcaccaccc acaggaacgg
    14221 gatcgaagcg atgttttgcg tgag
//
LOCUS       DBNGWZ010000416         6255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3199585, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000416 DBNGWZ010000000
VERSION     DBNGWZ010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3199585"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16CX_12570"
     CDS             <1..411
                     /locus_tag="AC16CX_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HIR3013381.1"
                     /translation="LEKDATGWDWIGINLDNGGALMAFRMRDKQGGHFWAGGALRDPN
                     GRLKIFSSDEVRFIPRREWKSALTGTAYPVAWLVRAGDREIGIEPLFDDQEHDTRAST
                     GTIYWEGAARALVEGKPVGRGYLELTGYWRRLNL"
     gene            complement(443..778)
                     /locus_tag="AC16CX_12575"
     CDS             complement(443..778)
                     /locus_tag="AC16CX_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013382.1"
                     /translation="MKCRSYECAALRRHCAGETRTPQSPASAGLSIFEVLSKAQGKKG
                     GDGMQVLSLHPQQGHGIDLTVDARLLHLFVKMLRDAVARADWEIPLALHEEGEQTAAA
                     EAVSPRKLN"
     gene            complement(942..3545)
                     /gene="clpB"
                     /locus_tag="AC16CX_12580"
     CDS             complement(942..3545)
                     /gene="clpB"
                     /locus_tag="AC16CX_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR3013383.1"
                     /translation="MRFDKFTTKFQQAFADAQSLAVGNDNPYIEPQHLLAALVSQQDG
                     GTASLLARAGVNVGGLRTALKSAIERLPKVEGAGGEVNVSRELGNLLNLTDKEAQKRG
                     DQFIASELFLLALIQDKGETGRLLKQHGGGRDAIEKAIHAVRGAETVQNPEAEGSREA
                     LKRYTLDITERARTGRLDPVIGRDDEIRRVIQILQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLKNKRVLSLDMAAMLAGAKYRGEFEERLKSVLKEIAQDAGQTIVF
                     IDELHTMVGAGKAEGAIDAGNMLKPALARGELHCVGATTLDEYRKHVEKDAALERRFQ
                     KVLVDEPSVEATIAILRGLQERYELHHGVDITDPAIVAAAELSHRYISDRFLPDKAID
                     LIDEAASRIKMEIDSKPEEMDRLDRRLIQLKIEREAMKKERDEASRKRLAALESEIKT
                     LEKDYSDLEEIWKAEKAQVQGAAHIKEEIDRVRAEIAQLQRQGKFDKVAELQYGKLPQ
                     LEAQLKQADKPKAGKLRLLRTQVGAEEIAEVVSRWTGIPVSKMMQGEREKLLQMEDKL
                     HQRVIGQDEAVKLVSDAIRRSRAGLSDPKRPYGSFLFLGPTGVGKTELCKALAEFLFD
                     SEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTELVRRKPYSVILLDEIEKA
                     HHDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGSQMIQQMAGDEYQLIKTAV
                     LGEVKAQLRPEFVNRIDEIIVFHALDEKQIQDIAKIQLRHLEGRLAALDIRLEVTATA
                     LKELAAAGFDPVYGARPLKRAIQAGIENPLAKAILEGRFAARDMVRVDHKKSGFTFEK
                     AELPQKQSVHA"
     gene            3786..4025
                     /locus_tag="AC16CX_12585"
     CDS             3786..4025
                     /locus_tag="AC16CX_12585"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR3013384.1"
                     /translation="MGAVCLRSGDLRARLPRYSPTTLFPYVVMDRMMIWDAATHTSSL
                     TVVFAGALMVPPFIIGYTAYAYRVFRGKASARPYD"
     gene            4532..6061
                     /locus_tag="AC16CX_12590"
     CDS             4532..6061
                     /locus_tag="AC16CX_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015558.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR3013385.1"
                     /translation="MAAQKWRTEDWVAVYLGFFIILATLVIFSGKFFDLRNVTSSFRW
                     TTDAQIASRTADWSAALDGVAKEAEAKGEKGKQLAARAADMKAALQKGDRKAIEKAAG
                     DLSKAGGRNTVAGTLGSEIRGHATATAATKVFTWDNLQKAVWLGVAWLVVATVGIAVV
                     GGKAGAFVIGFPVVFVLAWIGRVLAGNGLFIEYGVEYVIFALLAGLLISNTIGVPDWL
                     KPAVQTEFYIKTGLVILGAGLLFLEIIQAGALGIIQAVLVVFVIWYVCFWIARKLRVD
                     DDFAAMLSSSVAICGVSAAIATCGAIQGDKKKLSYVTSLVLIVAVPMMIIMPWLVKAF
                     QIPEIVAGAWLGGTLDTTATVVAAGALISDAAMKTGVIVKFSQNALIGVAAFILVVWW
                     TYKAGAKSGQKPDAHIIWERFPKFVLGFLVASIVFSFFVPTAMVDGTKGALAEFRTWW
                     FALAFVSIGLETKFVDLATMEGGRPAVAFIAAQIVNVFWTLLLAYLLFGGIIFPVPDI
                     K"
BASE COUNT          983 a         2001 c         2016 g         1255 t
ORIGIN      
        1 ctcgagaagg acgccacggg atgggactgg atcggcatca acctcgataa cggcggcgcg
       61 ctgatggcct tccgcatgcg cgacaagcaa ggcggccatt tttgggcggg tggcgcactg
      121 cgcgatccca atggccgcct taaaatattt tcatctgatg aagtgcgttt catcccccgc
      181 cgcgagtgga aatcagcgct taccgggacg gcgtacccgg tcgcgtggct cgtgagggcg
      241 ggagatcggg aaatcgggat cgaaccgctg ttcgacgacc aggagcacga cacccgtgcc
      301 tcgaccggca cgatctactg ggaaggcgcg gcacgtgccc tggttgaagg caaacccgtg
      361 ggacgcggtt atctggaact caccggttac tggcggcgac tgaatctcta gccgatccga
      421 cgcgacggct gcgcgatccg tctcagttca gtttgcgggg cgacacggct tcggcggctg
      481 cagtttgctc accctcctcg tgcagagcca gagggatctc ccagtcggcg cgggcaaccg
      541 catcgcgcag catcttcacg aagagatgga ggagcctcgc gtcgaccgtc aggtcgatgc
      601 cgtgcccctg ctgcggatgc aggctcagca cctgcatgcc gtcgccgccc ttcttgccct
      661 gcgccttcga taggacctcg aaaatggaaa gccccgccga agcggggctt tgtggcgtcc
      721 gggtttcccc ggcgcagtgc cgccggagag cggcacactc gtacgaacgg catttcaccg
      781 ttccggggat gcgctgggtc cggtgtaccg ccgaaccttc tcaccgatat ccggacggtc
      841 gcccggaatt tcggcttgcc cccggaccag gtacccgatc cggaagttcg cgcgctttcc
      901 tgattccttt ttacacgtcc cgcgcggcgc gctccgcatc gtcaagcatg aacgctttgt
      961 ttctgcggca gttccgcctt ttcgaaggtg aagccgcttt tcttgtggtc cacgcggacc
     1021 atgtccctgg cggcaaacct gccttcgagg atcgccttgg cgagaggatt ctcgatgccc
     1081 gcctggatcg cgcgcttcaa ggggcgcgcg ccgtaaaccg ggtcgaaacc ggccgcggca
     1141 agttccttta gcgccgtggc agtcacttca agccggatgt ccagtgccgc aagccggccc
     1201 tcgagatgcc gcagctggat tttcgcaatg tcctggattt gtttttcatc cagggcatgg
     1261 aacacgatga tctcgtcgat ccggttgacg aactccggcc gcaactgcgc cttcacctcg
     1321 cccagcactg cggtcttgat gagctggtac tcgtcgcccg ccatctgctg gatcatctgg
     1381 ctgcccaggt tggaggtcat cacgatcacg gtgttcttga agtccacggt gcggccctgc
     1441 ccgtccgtca tgcggccgtc gtcgagcacc tgcagcagca cgttgaacac gtcgtggtgc
     1501 gccttctcga tctcgtcgag caggatcacc gaatagggct tcctccgcac cagctcggtg
     1561 agatatccgc cctcctcgta acccacgtag ccgggaggcg cgccgatcag ccgcgcgacc
     1621 gagtgctttt ccatgaattc cgacatgtcg atccggatca gatggtcctc ggagtcgaac
     1681 aggaattcgg cgagcgcctt gcacagctcg gtcttgccca cgccggtcgg gcccaggaac
     1741 aggaacgagc cgtacgggcg tttggggtcg gagagccccg cgcgcgagcg gcggatagcg
     1801 tcggagacga gtttcaccgc ctcgtcctgg ccgatcacgc gctggtggag cttgtcctcc
     1861 atctgcagca gcttctcgcg ctcgccctgc atcatcttgg acaccgggat gccggtccag
     1921 cgcgaaacca cttcggcgat ctcttccgcg ccgacctggg tgcgaagcag cctgagtttg
     1981 cccgctttgg gtttatcggc ctgcttcagc tgcgcctcga gctgcggcag cttcccgtac
     2041 tgcagctcgg cgaccttgtc gaacttgccc tgccgctgca gttgcgcgat ctcggcgcgc
     2101 acgcggtcga tttcctcctt gatgtgcgcg gccccctgca cctgggcctt ttccgccttc
     2161 cagatctcct cgagatcgga gtagtccttc tcgagcgtct tgatctcgct ctccagcgcc
     2221 gcgagccgct tgcgggaggc ctcgtcccgc tccttcttca tcgcctcacg ctcgatcttc
     2281 agctggatca gcctgcggtc gagccggtcc atctcctcgg gcttggagtc gatctccatc
     2341 ttgatgcgcg aggccgcctc gtcgatgagg tcgatcgcct tgtccggcag gaagcggtcg
     2401 gaaatgtagc gatgcgagag ctccgccgcc gcgacgatcg ccgggtcggt gatgtccacc
     2461 ccgtggtgca gctcgtacct ttcctgcagg ccgcgcagga tggcgatggt cgcctcgacc
     2521 gacggctcgt ccaccagcac tttctggaag cggcgctcga gcgcggcgtc cttttccacg
     2581 tgcttgcggt actcgtcgag cgtggtggcg cccacgcaat gcagctcgcc gcgcgcgagc
     2641 gcgggcttga gcatgttgcc ggcgtcgatt gcgccctcgg ccttgcccgc gcccaccatg
     2701 gtgtgcagct cgtcgatgaa gacgatggtc tggccggcgt cctgggcgat ttccttcagc
     2761 accgacttca accgttcctc gaattcaccg cgatacttgg cgccggccag catcgcggcc
     2821 atgtccaggg agagaacccg cttgttcttg agcgattccg gcacttcgcc gttcacgatg
     2881 cgctgcgcga gcccttccac gatcgcggtc ttgccgacgc ccggctcgcc gatcagcacc
     2941 gggttgttct tggtgcgccg ctgcaggatc tggatcacgc gccggatctc gtcgtcgcgc
     3001 ccgattaccg gatcgagcct gccggtgcgg gcgcgttcgg tgatgtccag cgtgtacctc
     3061 ttcagcgcct cgcggctccc ttcggcctcg ggattctgaa cggtctcggc cccgcgcacg
     3121 gcgtggatcg ccttctcgat cgcgtctcga ccgccgccgt gctgcttgag cagcctcccg
     3181 gtttcgccct tgtcctggat cagcgcgagc aggaacagct ccgaggcgat gaactggtcg
     3241 ccgcgcttct gcgcttcctt gtcggtgagg ttgaggaggt tgccgagctc gcgcgagacg
     3301 ttgacctcgc cgcccgcgcc ttcgaccttg ggcaggcgtt cgatcgcgct cttgagcgcc
     3361 gttcgcagcc cgccgacatt gacgcccgcg cgggcaagca gcgacgccgt gccgccgtcc
     3421 tgctggctga ccagcgcggc gagcaggtgc tggggctcga tgtacggatt gtcgttgccg
     3481 accgccaggc tctgggcgtc ggcaaacgcc tgctgaaact tggtcgtgaa tttgtcgaag
     3541 cgcatatcgt tgccctaaca gtcggcccgc gtgtgttgaa cggcagcatc gctgcccgct
     3601 cgaacacgcg cagttaaacc ggcgcgtaag cgtccggttt taatgggata ttgggcatgg
     3661 tacgccgttt tcaaggccat ggccggcaat tatcgctgaa gcgcgctgcc tggcgcgaga
     3721 gcagcggcct ggctgtgggt ggggttcctc accgggcggt tgaaacggaa cgagcccgcc
     3781 cctgaatggg ggctgtttgc ctgcgcagtg gcgatctgcg tgctcgcctt cctcggtact
     3841 cgcctacaac cctgtttccc tacgtggtga tggaccgcat gatgatctgg gatgccgcga
     3901 cgcatacgtc ttcgctcacg gtggtgttcg ccggcgcgct catggtgccg ccgttcatca
     3961 tcggttatac cgcgtacgcc taccgcgtct tccgcgggaa ggcctcggca aggccatacg
     4021 attaagaaaa caaacgttgt cattgaagat atttatgcgt tgccaggggt tgtaggcgcg
     4081 aagaccgcat gccacatgac ggaaacatgc ttgacttcca agtgggggcg actaacattg
     4141 ttcgcgttcg actgttgctc ttgcagcttg aagcgtgcag ccgcaacacc ggctgtgccg
     4201 acaacaacca gcgcgcgcgg tgcatgcgcg tggcataagc ggctgtccgg gccgcctgca
     4261 atcgctccgc agcgcctggc atgcgacgtt gcagaatagg aggagcatat ggtctatccg
     4321 caaaagatcc cgtaggtgca tttcccccgc ttggccgtca tgctcgcgcc ccttccaccc
     4381 aggaggaccc tgttggcgag ctaagcacat cccggacccc ggtcaatgcg ccggcgggat
     4441 gcagaccatg tgccgcgttg ccgggaggcg gcagccatca gcttggcaat gaagctgatt
     4501 acgggaggca aatcagataa ggaggcagac catggcagca caaaaatggc gcacggagga
     4561 ctgggttgcg gtctatctcg ggtttttcat catcctcgcc acgctggtaa tcttcagcgg
     4621 gaagttcttt gatctcagga acgtgacgtc gtcgttccgc tggacgaccg acgcgcaaat
     4681 cgcctcgcgc accgccgatt ggagcgcagc tctggacggt gtcgccaagg aggccgaggc
     4741 gaagggcgag aagggtaagc agctcgcagc cagggctgcc gacatgaagg ccgcgctgca
     4801 gaagggcgac cgcaaggcga tcgagaaggc ggcgggcgac ctctccaagg cgggcggacg
     4861 caataccgtg gccggaacgc ttggctccga gattcgcggg cacgcgaccg cgaccgcggc
     4921 aaccaaggtc ttcacctggg acaacctgca gaaggcggtc tggctcggcg tggcgtggct
     4981 cgtcgtcgcc accgtcggca ttgcggtggt cggcggcaag gcgggcgcgt tcgtgatcgg
     5041 gttcccagtc gtgttcgtcc tggcgtggat cggtcgcgtg ctggcgggaa acggcctgtt
     5101 catcgaatac ggcgtggagt acgtgatctt cgcgctgctc gcggggctgc tcatcagcaa
     5161 taccatcggc gtcccggact ggctcaagcc cgcggtgcag accgagttct acatcaagac
     5221 cggtctcgtg atcctcggtg ccgggctgtt gttcctggag atcatccagg ccggcgcgct
     5281 cgggatcatc caggcggtcc tcgtggtgtt cgtgatctgg tacgtgtgct tctggatcgc
     5341 gaggaagctg agggtcgacg acgattttgc ggcgatgctc tcatcctcgg tcgcgatctg
     5401 cggcgtgtcg gcggcgatcg ccacctgcgg cgcgatccaa ggcgacaaga agaagctctc
     5461 ctacgtcacc tcgctcgtgc tgatcgtcgc ggtgcccatg atgatcatca tgccttggct
     5521 ggtgaaggcg ttccagatcc ccgaaatcgt cgccggcgcc tggcttgggg gcacgctgga
     5581 caccactgcc accgtcgtcg ccgcgggagc gctcatcagc gacgcggcga tgaagaccgg
     5641 cgtgatcgtg aagttctcgc agaacgcgct gatcggggtc gcggccttca tcctcgtggt
     5701 ctggtggacc tacaaggccg gagcaaagag cggccagaag cccgatgcgc atatcatctg
     5761 ggagcgcttc ccgaaattcg tgctcgggtt cctggtggcc tccatcgtgt tctcgttctt
     5821 cgtgcctacc gccatggtag acggcaccaa gggcgcgctt gccgagttca ggacctggtg
     5881 gttcgccctg gcgttcgtgt cgatcgggtt ggagaccaag ttcgtcgacc tcgcgaccat
     5941 ggaaggcggg cgcccggcgg tggcgtttat cgcggcgcag atcgtgaacg tcttctggac
     6001 gctgcttctc gcctacctgc tgttcggcgg catcatcttc ccggtgcccg acatcaagta
     6061 gccgcggcgc cgtgaattca agccccgcgc gggcggccgg tccgcctgcg cggggcttat
     6121 catttcagga agccgccgag tgcctcggag tgaccgccat gcccggctgg gtcgcgcgcc
     6181 tgttctcgaa ggagaggaac ctcaggatgg tctgggaggt ggagaaacgc gggcggcgtt
     6241 cgatgctcgt cggca
//
LOCUS       DBNGWZ010000417         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1059045, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000417 DBNGWZ010000000
VERSION     DBNGWZ010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1059045"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="AC16CX_12595"
     CDS             complement(<1..881)
                     /locus_tag="AC16CX_12595"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3013386.1"
                     /translation="MNETVANDKLGAFCRENHAEVKGAGTGPLAGLTFGVKDIYDVAG
                     AKTGFGSPDWLRTHDAAARTAPVVRRLLDAGADLVGKTHTDEITWSLNGENAHYGTPV
                     NVNAPGRIPGGSSSGSAAAVAGGLCDFALGSDTGGSVRAPASYCGIYGIRPTHGRVLL
                     EGACPLAPSFDTCGWFARSAELLERVGRVLLGEEAGATPRRLLLAQDAFALAGEAVTR
                     ALDPAVKRIGALLGKPESVIVAADGLPQWFNVFRILQGAEAWAAHGEWVKRVKPDLGP
                     GIKQRVQWAADLDPSLVG"
     gene            complement(1018..>2211)
                     /gene="mpl"
                     /locus_tag="AC16CX_12600"
     CDS             complement(1018..>2211)
                     /gene="mpl"
                     /locus_tag="AC16CX_12600"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HIR3013387.1"
                     /translation="PDIFVIGNVVTRGNPLMEEILDRGLPYISGPQWLRENILAGKWV
                     VAVAGTHGKTTVSAMIAWILEHTGLEPGFLIGGVPENFGVSARLTNSRFFIVEADEYD
                     TAFFDKRSKFVHYPARTAVLNNLEFDHADIFPDLAAIETQFHHFVRTLPASGLIVANG
                     TDEALTRVLARGCYTPFERFGAADGWSAASAARGGFDVLWRGEQQGRVQWPLQGAHNQ
                     ANALATIAAARHCGVAPARAIAALGAFDGVKRRMEVRGTVNGITVYDDFAHHPTEIAI
                     TLEGLRDKMNSGRLIAVFEPRSNTMKLGVMKERLAASLAAADRVFCYSAGLKWDARAA
                     LAPLGARATATGDFDQLLEAVAAVSRPGDHIVIMSNGGFNGIHDKLLARLARGKPGER
                     EGGRA"
BASE COUNT          363 a          769 c          707 g          372 t
ORIGIN      
        1 ccgaccaggc tcgggtcgag gtcggcggcc cactggacgc gctgtttgat cccgggcccg
       61 agatcgggct tgacgcgctt gacccactcc ccgtgcgcag cccacgcctc ggccccctgc
      121 aatatgcgga acacgttaaa ccactgcggc aggccgtcgg cagccacgat cacggactcg
      181 ggttttccca gcaacgcgcc gatccgcttg actgcgggat cgagcgcgcg agtgaccgcc
      241 tcgcccgcca gcgcgaaggc gtcctgcgcc agcagaagcc ggcgcggcgt agcgcctgct
      301 tcctcgccga gcagcacgcg gccgacgcgc tcgagcaatt cggcgctgcg cgcgaaccag
      361 ccgcaggtat caaagctcgg cgcgagcgga cacgcgccct ccagcaaaac gcgcccatgc
      421 gtggggcgta tgccgtagat cccgcagtag ctcgccggcg cgcgcactga accgcccgtg
      481 tcgctgccca gcgcaaagtc gcacaggccg cctgcaaccg ccgccgccga gccgctcgac
      541 gagccaccgg gaatgcgccc cggcgcgttg acgttgaccg gcgtgccgta atgcgcgttc
      601 tccccgttca ggctccaggt gatctcatca gtatgggtct tgcccaccag atcggcgccg
      661 gcgtcgagca ggcgccgcac caccggcgcg gtccgcgcag ccgcgtcgtg cgtgcgcagc
      721 cagtcggggc tgccgaagcc ggtctttgcg cccgcaacgt cataaatgtc cttcacgccg
      781 aaagtgaggc cggcgagcgg ccctgtaccc gcacccttta cctcggcgtg attctcgcgg
      841 cagaacgcgc cgagcttgtc gttggcgacg gtttcgttca tagaaacatg tgagatcggg
      901 agatcgggag atcgagaaac cgggagatcg tgaagtcggg agatcgggag atcgaaagaa
      961 ggctccgttt ccctctcacg ctctcacgcc ctgacgccct cccgggttct atcgctctca
     1021 cgctctcccg ccctcccgct ctcccggctt ccctcgggcc agccgggcca ggagcttgtc
     1081 gtgtatgccg ttgaaaccgc cgttgctcat gatgacgatg tggtcgccgg gacgcgatac
     1141 cgccgcaacc gcctcgagaa gctggtcgaa atctccggtc gccgtcgccc ttgcgccaag
     1201 cggcgcgagc gcggcgcgcg cgtcccattt gaggccggcg ctgtagcaga acacgcgatc
     1261 cgctgcggcg aggctcgccg ccagccgctc tttcatcacg ccgagcttca tggtgttcga
     1321 gcgcggttcg aacaccgcga ttagccgccc gctattcatt ttgtcgcgca ggccctcgag
     1381 tgtaatcgcg atctccgtcg gatgatgcgc gaaatcatcg taaacggtga tgccgttcac
     1441 ggtgccgcgc acttccatgc ggcgcttgac gccgtcgaag gcgccgagcg cggcgatcgc
     1501 ccgcgccggc gcgacgccgc aatgccgcgc cgccgcgatc gtggcgagcg cgttggcctg
     1561 gttgtgcgcg ccttgaagcg gccattgcac gcggccctgt tgttccccgc gccacagcac
     1621 gtcaaagccg cctcgcgcag cggatgccgc gctccagccg tcagccgcgc cgaagcgctc
     1681 gaacggcgta tagcatccgc gcgccagcac gcgcgtgagc gcctcatcgg ttccgttcgc
     1741 cacgatcagg ccgctggccg gcagcgtgcg cacgaaatgg tggaactgcg tctcgatcgc
     1801 cgcgaggtcg gggaagatgt cggcgtggtc gaattccagg ttgttgagca ccgccgtgcg
     1861 cgccgggtag tgcacgaact tcgagcgctt gtcgaagaac gcggtgtcgt actcgtcggc
     1921 ctcgactatg aaaaagcggc tgttggtgag tcttgcggac acgccgaagt tttccggaac
     1981 accgccgatc aggaagcccg gctcgaggcc ggtgtgctca aggatccagg caatcatggc
     2041 ggagacggtg gttttgccat gcgtcccggc caccgcaacc acccattttc cggcaagaat
     2101 gttttccctc agccattgcg gacctgaaat gtaaggcaat ccccggtcga ggatttcctc
     2161 catcagcgga ttgccgcgag taacgacgtt cccgatcacg aaaatgtcgg g
//
LOCUS       DBNGWZ010000418         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2569593, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000418 DBNGWZ010000000
VERSION     DBNGWZ010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2569593"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16CX_12605"
     CDS             <1..329
                     /locus_tag="AC16CX_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013388.1"
                     /translation="PIDPHFPHPLVPFFGAYPQPLKALLMTASGLGAGKRSGSYADAV
                     KRLRHYNPLCASDLKRLFPSGTLLKEQTFGLTLSLVIHRGLGYEGARRAPPYDYADDG
                     RLIDSA"
     gene            complement(343..939)
                     /gene="ahpD"
                     /locus_tag="AC16CX_12610"
     CDS             complement(343..939)
                     /gene="ahpD"
                     /locus_tag="AC16CX_12610"
                     /EC_number="1.11.1.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0160319 - lipoyl-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase, AhpD family"
                     /protein_id="HIR3013389.1"
                     /translation="MSMQTLKDRLPEYAKDLRLNLSTLASESILDEEKKAGTFVAAAI
                     ASRNTHVIRAALAEFGPKMDPAALKAAQAAAAIMAMNNVYYRFTHLIETEEYAKLPAK
                     LRMNAMANPGTAKENFELWSLAVSAVNGCGKCLVAHERVLRQAGLSTEQIQAAVRIAA
                     AVHAAAVTLEAEDAVADILIQRMQQHEKNAWMLRSLQR"
     gene            complement(951..1490)
                     /locus_tag="AC16CX_12615"
     CDS             complement(951..1490)
                     /locus_tag="AC16CX_12615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3013390.1"
                     /translation="MLTVGDRVPSFDLQAVVSTDQEKAFIRITEESDAGKWKVIFFWP
                     KDFTFVCPTEIAAFGRLHEEFKDRDAVIYGVSIDSEFVHLAWRRSHPDLKDLPFPMLA
                     DVKREITGAFGVLDKKEGVALRATFIVDPDGIIRFVSVNDLNVGRNVDEVLRTLDALQ
                     TDELCPCNWHKGEEFLKAA"
     gene            1691..2638
                     /locus_tag="AC16CX_12620"
     CDS             1691..2638
                     /locus_tag="AC16CX_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HIR3013391.1"
                     /translation="MPALPTLRQLGYLVALADRLNFRAAAEARFVTQSTLSAGIKELE
                     TVLGVQLVERDKRRVRLTAVGEEVTARARELLAAATDLAEAARSAAAPLSGPLRLGAI
                     PTIAPFLLPEVLPPLRRIHRALKLYLREDLTERLLERMRAGSLDIALIALPYDTGDLH
                     VRELFKDEFWFVAREGDPAAREKEIVIRKLDTSEVLLLEEGHCLRDHAIAACGPRRDR
                     WEAKVEATSLYTLIQMVEGGLGVTLLPEITLKAGILKGTRLIARPFAPPAPSRTLALV
                     ARRTSPRRRDAELLAEFILEQRRLSGRPGIPALRRAPGG"
     gene            complement(2663..3007)
                     /locus_tag="AC16CX_12625"
     CDS             complement(2663..3007)
                     /locus_tag="AC16CX_12625"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HIR3013392.1"
                     /translation="MNKSIVKGWKIALITGAFAFLVNPAWAEVVSTEQIVTQSDRDRV
                     ETFLKRDGVVKELKALGIAPELATQRVNALTDEEVRVIAGKMDTLAAGGALDKTDWVI
                     IILLVVVLLLAL"
     gene            3272..4558
                     /locus_tag="AC16CX_12630"
     CDS             3272..4558
                     /locus_tag="AC16CX_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3013393.1"
                     /translation="MKRIVFATFGSLGDLYPYVAIARELNRRGHRPVVASFAEFREAV
                     KTAGVEFAPLRPAMSTFGERAAMMERLVDPWGGPELLVRGIFMPHLRESYEDLARACR
                     DADLLVTHPLAFAGPLLAQKEGVRWVSTALSPMTMFSAIDPPLFPAAPWMRWARRLGV
                     SAYRMVFRIPRAMVRRWESPLRAFRAELGLPPTDALAQLEGQYSPLRHLALFSHVLAA
                     PQPDWPPNTVVCGFPRYDGAPRDARTHSELEAFLASGEPPVVFGLGSSAVMVAGGFWR
                     AATEAARNLGRRAILLTGDPPGSLGPVPPTVKVFQYLPYSAVFPRAAAAVHSGGIGTL
                     AQALAAGRPQLIVPVAFDQPDNARRAVGLGLARSLPFRKAGAGPLARALRELLASADA
                     AARARTIGEEVAREDGARRSADALEEILRTGARQIP"
     gene            4619..5023
                     /locus_tag="AC16CX_12635"
     CDS             4619..5023
                     /locus_tag="AC16CX_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013394.1"
                     /translation="MAQRPDERGGEPKMDPASLYREEIITDRKVGTIRMLTPVKSDGS
                     ADASRAVLYIGEAQLLTSVGTLPINFEIEAQSLDEAVKKFAEVAKDAVEHTVKDLQEL
                     RRQAASSIVVPQGGMGGLGPGGMPGGGKIQIP"
     gene            5151..5819
                     /locus_tag="AC16CX_12640"
     CDS             5151..5819
                     /locus_tag="AC16CX_12640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013395.1"
                     /translation="MKRISVLLRALALVLFTLATAPLVAQSVEHSQHSFGGAEKWAKV
                     FDDPERDAWQKPHEVIQALKLAPDASVADIGSGTGYFAVRLAHRVPQGRVYGVDTEPD
                     MVRYLGERAKREGLANLTAVAAKPSDPRIPSAVDLAILVDTYHHVPDRERYFRNLQKS
                     LKPGGRLAIIDFKPDSPVGPPKQGRIPADQVKQELSRAGYVLEKEHGFLPYQYFLEFM
                     PRKR"
     gene            complement(5843..6817)
                     /locus_tag="AC16CX_12645"
     CDS             complement(5843..6817)
                     /locus_tag="AC16CX_12645"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013396.1"
                     /translation="MKSSFFVIFVGAAAVSLLPGMIGHAQNYPTRPVRIIAASTGTTG
                     DLLARYLAQQLTERWGKQVVVENRSGAGAVIAAEVAAKAAPDGYTLHLGQLASFSAAV
                     SLYRKLPYDPVKDFAPITLYAQMPLMFIAHPSVPAANLRELIDYMKNRPGAINVSSAG
                     NGTGSHLTTELLSHAAGLKLVHVPYKGTGAAITALVSGEAQVSSIVLPSALPQVKAGK
                     VKAYAVTSRNRFAGAPDIPTAGEAGLPGFESTTWFGMFAPVRAPEALVGRLNRDMVEI
                     LRTPATQAWLLAQGAEPSPTTPQEFAAFIKSETAKWKKVIEASGARVD"
BASE COUNT         1140 a         2368 c         2186 g         1259 t
ORIGIN      
        1 cgccgatcga cccgcatttt ccccatcccc tggtgccttt cttcggcgcg tatccccagc
       61 cgctgaaggc gctgctcatg acggcgagcg gcctgggcgc gggcaagagg tccgggagct
      121 atgccgacgc cgtgaaacgc ctgaggcact acaatccgct ctgcgcttcg gacctgaagc
      181 ggctgtttcc ctccggtacg ctgctcaagg agcagacttt cggcctcacg ctgtcgctcg
      241 tcatccatcg cggcctcggg tacgaggggg cgaggcgggc gccgccgtac gactacgccg
      301 acgacggccg gctcatcgat tcggcgtaga ccgattcgag gatcagcgct gcaggctgcg
      361 gagcatccag gcgttcttct cgtgctgctg catgcgctgg atcaggatgt cggcgaccgc
      421 gtcctcggcc tcgagcgtca ccgccgcggc gtgcaccgcg gcggcgatgc gcacggccgc
      481 ctggatctgc tcggtgctta gccccgcctg gcgcagcacg cgctcgtgcg cgaccaggca
      541 cttgccgcag ccgttcaccg cggacacggc gagcgaccac agctcgaagt tctccttcgc
      601 ggtgccggga ttcgccatcg cgttcatgcg cagcttcgcc ggcagcttgg cgtactcctc
      661 cgtctcgatg agatgcgtga accggtagta cacgttgttc atcgccatga tggccgcggc
      721 ggcctgcgcc gccttgagcg ccgcgggatc catcttgggc ccgaactcgg cgagcgcggc
      781 gcggatcacg tgggtgttgc gcgacgcgat tgcggccgcg acgaacgtgc cggccttctt
      841 ctcctcgtcc aggatggact cggatgcgag cgtcgagagg ttcaggcgca ggtccttggc
      901 atactcgggc aggcggtctt tcagcgtttg catcgacatc gtgctctccc tcatgcggcc
      961 ttcaggaact cctcgccctt gtgccagttg cacgggcaca gctcgtcggt ctgcagcgcg
     1021 tccagcgtgc gcagcacctc gtcgacgttg cgcccgacgt tgaggtcgtt caccgacacg
     1081 aagcggatga tcccgtccgg atcgacgatg aaggtcgcgc gcagcgccac gccttccttc
     1141 ttgtcgagca cgccgaacgc gccggtgatc tcgcgcttga cgtcggcgag catcgggaag
     1201 ggcaggtcct tcaggtcggg atgcgagcgg cgccacgcca ggtggacgaa ctcgctgtcg
     1261 atcgagacgc cgtagatcac cgcgtcgcgg tccttgaatt cctcgtgcag cctgccgaag
     1321 gccgcgatct cggtcgggca cacgaaggtg aagtccttgg gccagaagaa gatcactttc
     1381 cacttgccgg cgtcggactc ctccgtgatg cggatgaatg ccttctcctg gtcggtggac
     1441 accaccgcct ggaggtcgaa gctcgggacg cggtcgccta ctgtcaacat tgccatctcc
     1501 attcatcagg ttgatcaaaa cggatgcgag tatgacgcgc cccttttgat cgctccaacg
     1561 aataatgtga gttagaatga tcgagattat cgattaagcg tgatgggtga tgagtgatgg
     1621 gtgacgggtc tcccgcactg cgggggtcat ccttcatcct tctgccttca tccttcacgc
     1681 gaagcgtccg atgcccgccc tcccaaccct gcgccagctc ggctacctgg tcgcgctcgc
     1741 cgaccgcctc aacttccgcg cggcggcgga ggcgcggttc gtcacccagt ccacgctctc
     1801 ggccggcatc aaggaactcg agaccgtgct cggcgtgcag ctggtcgagc gcgacaagcg
     1861 ccgcgtgcgc ctcaccgccg tgggcgagga agtcaccgcc cgtgcccggg aactgctggc
     1921 ggccgcgact gacctggcgg aagcggcgcg gagcgctgcc gcgccgctct ccggcccatt
     1981 gcgactcggt gcgatcccca cgatcgctcc gtttctgctg cccgaggtcc ttcccccgct
     2041 gcgtcggatc caccgggcgc tgaagctcta tctgcgcgag gatctgaccg agcgcctgct
     2101 cgagcgcatg cgcgcgggaa gcttggacat cgcgctgatc gccctaccct acgacaccgg
     2161 ggaccttcac gtgcgcgagc tgtttaagga tgaattctgg ttcgtggcgc gcgagggcga
     2221 ccccgccgcg cgggagaagg aaatcgtcat ccgcaagctc gacacgagtg aggtcctgct
     2281 gctggaggaa ggccattgcc tgcgcgatca cgcgatcgcc gcctgcggcc ctcgtcgcga
     2341 caggtgggaa gccaaggtgg aagccaccag cctctatacc ctgatccaga tggtcgaagg
     2401 ggggctcggc gtgaccctgt tgcccgagat cacgttgaag gccggcatac tcaagggcac
     2461 gcggctcatc gcccgcccgt tcgcgccgcc cgctccttcg cgcacgctcg cgctggtggc
     2521 gcgccgcacg tctccgcgcc ggcgggatgc ggaattgctc gccgagttca tcctcgagca
     2581 gcggcgcctc agcgggcggc ccgggatacc ggcgctccgc cgcgcgcccg gcggttgatg
     2641 cgccgcgcgg cggagccgca ccttacaacg cgaggagcag cacgaccacg agcaggatga
     2701 taatgaccca gtcggtcttg tcgagcgcgc cgcctgccgc cagcgtgtcc atcttgccgg
     2761 cgatcacccg tacctcctca tcggtcagcg cattgacccg ctgcgtggcg agctcggggg
     2821 cgataccgag cgctttcagc tcctttacca cgccgtcgcg cttgaggaaa gtctccaccc
     2881 ggtcgcgatc ggactgcgtc acgatctgtt cagttgaaac gacttcggcc cacgccggat
     2941 tgaccaggaa tgcgaatgca cccgtaatga gcgctatctt ccagcctttc acaatgcttt
     3001 tgttcatggc gtatcctcct gtaaaaacgt gcaaacttgg cggttactct gctcccgtcc
     3061 ggcaaccatt atgccgttaa catcccacaa taggacaacg acggagtcat tttgatcccg
     3121 cacggacggc caaaaccgtc tccatccggc gacagtttcg accgccgacg cccgcccgcc
     3181 aaccccgaaa gcatgaatgg tgaatagtga atcgacgacc cttcatgcct tcgaatcccg
     3241 actcacgacc cacgaattac gctgttccgc catgaagcgg atcgtcttcg ccaccttcgg
     3301 ctcgctcggc gacctgtatc cctacgttgc gattgcacgc gagttgaatc gccgcggcca
     3361 ccggccggtg gtcgcgagct tcgccgagtt ccgcgaggcg gtcaagacgg cgggcgtcga
     3421 gttcgcgccg ctgcggcccg ccatgtccac cttcggcgag cgcgcggcga tgatggaacg
     3481 gctggtggat ccctggggcg ggcccgaact cctggtgcgc gggatattca tgccgcacct
     3541 ccgcgaaagc tacgaggacc tcgcgcgcgc gtgccgcgac gcggatctcc tggtcaccca
     3601 cccgctcgcc ttcgccgggc cgctgctcgc gcagaaggaa ggcgtgcgct gggtctcgac
     3661 cgccctctcg ccgatgacga tgttctcggc gatcgatcct ccgctctttc ccgccgcgcc
     3721 gtggatgcgc tgggcgcgcc ggctcggagt ctccgcctat cggatggtgt tccgcattcc
     3781 gcgcgcgatg gtcaggcgct gggagtcgcc gctgcgcgcg ttccgcgccg agctcgggct
     3841 gccgccgacg gacgcgctcg cgcagctcga aggtcagtac tccccgcttc ggcacctggc
     3901 gttgttctcc cacgtgctcg ccgcgccgca gcccgattgg ccgccgaata ccgtcgtatg
     3961 cggcttcccg cgctacgacg gcgcgccgcg cgatgcccgc actcactccg agctcgaggc
     4021 tttcctcgct tcgggggagc caccggtcgt attcgggctg ggttcctccg ccgtcatggt
     4081 cgcgggcggg ttctggcggg ccgcgaccga agccgcgcgc aatctggggc ggcgcgccat
     4141 cctactgacc ggcgatccgc ccgggtctct cggtcccgtg cctcctaccg tgaaagtttt
     4201 ccagtacctt ccctattcgg cggtattccc gcgcgcggcg gcggcggttc actccggggg
     4261 catcggcaca ctcgcgcaag cgctcgcggc aggccgcccg caactgatcg tgccggtggc
     4321 attcgaccag cccgacaacg cgcggcgcgc ggtggggctc ggtcttgcgc gcagcctccc
     4381 cttccgcaag gccggcgccg gcccgctcgc gcgggcgctc cgcgaattgc tggcgagcgc
     4441 cgatgccgcg gcgcgcgcac ggacgatcgg cgaggaggtc gcgcgcgagg acggcgcccg
     4501 tcgatcggcc gacgcgctgg aagaaattct ccggaccgga gcccggcaaa ttccttaaaa
     4561 caggcggtga aattctgcta cgcttgcggt tatcgcaacg ctgaatgacg aggcagacat
     4621 ggcccaaaga cccgacgagc ggggcggcga gccgaaaatg gatcccgcgt ccctttaccg
     4681 cgaggagatc atcaccgacc gcaaagtggg cacgatccgc atgctgacac cggtgaaaag
     4741 cgacggctcg gccgacgcca gccgggccgt gctctacatc ggcgaggcgc agctgctcac
     4801 cagcgtcggc acgctgccga tcaacttcga gatcgaagcc cagtcgctcg acgaggcggt
     4861 gaagaagttc gccgaggtgg cgaaggacgc cgtcgagcac accgtcaagg acctgcagga
     4921 gctgcgccgc caggcggcct cctcgatcgt cgtgccgcag ggcggcatgg gcgggctcgg
     4981 gcccggcgga atgccgggcg gcggcaagat ccagattcca taagttcccg ccgcttcgcc
     5041 actccccgcc cgcgcaccaa gtatcaccgg gcggggaacc tatgttcgtc ggatcgatct
     5101 atgacagcgg gcgctttgcg cccgtatctc attcccgaaa ggcatccacc atgaagcgga
     5161 tttccgttct cctgcgcgcg ctcgcgctcg ttcttttcac gctggccacg gcccccctcg
     5221 tcgcgcagag cgtagaacat tcccagcaca gcttcggcgg cgcggaaaag tgggcgaagg
     5281 tattcgacga tcccgagcgc gacgcctggc agaagccgca tgaagtgata caagcgctca
     5341 agctcgcgcc cgacgcatca gtcgccgaca tcggctcggg caccggttat ttcgcagtgc
     5401 gcctcgcgca cagggtgcca caaggccgcg tctatggagt ggataccgaa cccgacatgg
     5461 ttcggtacct cggcgagcgc gcgaagcgcg agggcctcgc caacctcacc gccgtcgccg
     5521 ccaaaccgag cgacccgcgc attccgtccg cggtggacct cgccatcctc gtggatacct
     5581 atcaccacgt gcccgaccgc gagcgttatt tcaggaacct ccagaaatcg cttaaaccgg
     5641 gcggccggct cgcgatcata gacttcaagc cggattctcc cgtcggcccg ccgaagcaag
     5701 gccgcatccc ggccgaccag gtcaagcagg aactctcgcg cgcgggatac gtgctcgaga
     5761 aggagcacgg ttttctgccc taccagtact tcctcgagtt catgcctcga aagcgttgaa
     5821 tcgtggcacg gcaagaagta agtcaatcaa cccgtgcgcc ggatgcctcg attaccttct
     5881 tccatttggc ggtttcgctc ttgatgaacg cggcgaactc ctgaggcgtg gttggggagg
     5941 gttcggcgcc ttgcgcgagc agccaagctt gggtggcggg cgtacgcaga atttcgacca
     6001 tgtcacggtt gagcctgccg accaaggctt ccggcgcgcg caccggcgcg aacattccga
     6061 accaggttgt cgattcgaaa ccgggcaggc ccgcttcgcc ggcagttggg atgtcgggcg
     6121 cccccgcaaa acggttcctg ctggtaactg cgtacgcctt taccttgccg gccttcacct
     6181 gtggcaaggc actgggcaat acgatcgagg atacctgcgc ttcaccgctg actaatgccg
     6241 tgatcgcggc gccggtgcct ttgtaaggga catgcaccag cttgaggcca gcggcgtggc
     6301 tgagcagctc cgtcgtcaga tggctccccg tgccgttgcc tgcggaggaa acgttgatcg
     6361 cgcccgggcg atttttcata taatcgatga gttcccgcaa gttcgccgcc ggcactgagg
     6421 gatgcgcgat gaacatcaac ggcatttgcg cgtacagggt aatcggcgcg aaatccttga
     6481 cgggatcata cggcagtttc cggtagagac tgaccgcagc agagaatgat gcgagctgtc
     6541 caaggtgcaa ggtgtaacca tccggcgctg ctttggctgc cacttcagcg gcgatgacag
     6601 cgccggcgcc ggagcggttc tccaccacaa cctgcttccc ccaccgctcg gtaagctgct
     6661 gcgccagata acgcgctaga agatctccgg tcgtgcctgt cgacgcagcg atgatgcgaa
     6721 ccggcctcgt cgggtagttc tgtgcatgcc cgatcatccc gggcagcagg ctcaccgccg
     6781 ccgcgcctac gaatatcacg aagaacgagc tcttcatggc tcctccccat ggacgttggg
     6841 aagtaacgag tatatgaccg caggggttct tatttcaaat tctgaatttc cctggctccc
     6901 tttcagttca gctttgcttc tcatcactgc gtcacttcgt cactacgtca cct
//
LOCUS       DBNGWZ010000419         5139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1311918, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000419 DBNGWZ010000000
VERSION     DBNGWZ010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1311918"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="AC16CX_12650"
     CDS             <1..977
                     /locus_tag="AC16CX_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR3013397.1"
                     /translation="FPELAGYKLLPFGKGMCSDDTEHTCMLAQSLIETAGYSDADTMA
                     KKFASNFGWRLRFWLLGLPAGIGLATLRAILKLWIGFPARWSGVFSAGNGPAMRVALI
                     GVCHGDDVPRMRALVRAATRVTHTDPRAEHGALAVALAAHLASTGKDVAAGDYARKLR
                     ELLGDDGRELLELVDDVAQSVGRKETAADFAARIGCATGVTGYMLHTVPAALHVWLRR
                     QTDYRPALIETVRLGGDTDTVAAVVGALIGARVGKEGIPTEWLRDLWEWPRTKTWLDQ
                     LSVRLAERCKDHASGGAVSLNWVALLLRNALFIPLVLAHGFRRLLPPY"
     gene            complement(1100..2080)
                     /locus_tag="AC16CX_12655"
     CDS             complement(1100..2080)
                     /locus_tag="AC16CX_12655"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013398.1"
                     /translation="MSEGLSLRILAAVLAGGSMLFSACAFAQAWPGKPVRIIVPFGPG
                     GGTDIQARVLTTVFHEATGQTFVIENRTGASGLIGAEIAANSPPDGYTLLFSTATLTI
                     NTTLYGSRMKTDPVKDFAPVSWLTSTPLVLVVHPSVPANSVKELIALAKQKPAFLNSG
                     INVPGSTSHLSAEMLKQRAGIEAIIVPYKGGGPAIAALMSGEIDYLFATGPVASAALK
                     SGRVRALAVTTANKASAFPDLPTMNTFFPDFESDNWYAMWFPANTPRDIVTKMNGLIV
                     NALKNGKVREFMVKEGLDPVGSTPEELAAQIKREIVKYAEVIRKGNIKLQ"
     gene            complement(2272..3240)
                     /locus_tag="AC16CX_12660"
     CDS             complement(2272..3240)
                     /locus_tag="AC16CX_12660"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013399.1"
                     /translation="MNRSLLPQLVAVAVGFALGGAAYAQAWPVKPVRIIVPFGPGGGT
                     DIQARLLSKNFFESMGQTFIVENRSGAGGIIGAELTARAPADGYTILFTTASLAVNVT
                     LYGTRIKFDPLKDLAPVSWISSTPLVLVTHPSVPVKSVKELVALAKKSGTLNAGSNGA
                     GTTSHLSVEMLRQMTGVKVTHIPYKGGGPSMVALVSGETDLLFGTGPVATPQIKAGKV
                     KALAVTTAKKSSAFPELPTMTSIYPGFESDNWYAMFFPAGTPRPIIDKLNAEIAKALK
                     AKDILEFMPREALDPVASSPEELHANFKREIARYAKIITAGNIKVD"
     gene            complement(3298..4212)
                     /locus_tag="AC16CX_12665"
     CDS             complement(3298..4212)
                     /locus_tag="AC16CX_12665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013400.1"
                     /translation="MDQPKAAAKITGQEHWTHKGEVRLFLWEKRAAKPRGTILFVHGS
                     SMGSQPTFDLQVPGRPHSSAMDFFAERGFDAWCVDMEGYGRSDKKRDINCDIANGADD
                     LEAATDYIMKTRGTRQLLVYGISSGALRAALFAQRHPGRVKRLALDAFVWTGEGSPTL
                     AERRKKLPEFQAKNRRPIDRAFVRSIFNRDHPGSAEDKVIEAFADHILKLDDSVPTGT
                     YVDMCSKLPVVDPAKITVPTLIMRGQFDGIAGFDDLTEFFKRLPNPDKQFAVMAGIAH
                     ASFQQINYLMVYHILHSFFTQPEPIYRG"
     gene            4339..>5139
                     /locus_tag="AC16CX_12670"
     CDS             4339..>5139
                     /locus_tag="AC16CX_12670"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013401.1"
                     /translation="MTRLYEKARIGDIDIHYHLADYTDPWRKPPAETFLCYSGYCRSL
                     EFWHAWVPLLARDYRVLRMDPRGYGHSGKPGPGYRFEIEQLARDALGLMDHLGIERAH
                     WVGDSTGGAVGMSAALLAPRRISSITACNTTAKMAQQTVDTYAIGEQDQAAAIEKFGV
                     YEWCRRTLKHRVDISKIPPELGEWWARDMDRTPRHVATAVFRCFSTADLWPRLKEIRA
                     PTLLIVGDNCPEIRKRQLAEMAKTLPRGKLVNLEGYDYGIHFLAPERCV"
BASE COUNT          890 a         1653 c         1626 g          970 t
ORIGIN      
        1 tgtttcccga gctcgccggc tacaagctcc tccccttcgg caagggcatg tgctccgacg
       61 acaccgagca cacctgcatg ctggcgcagt ccctgataga aaccgccggc tatagtgacg
      121 cggacaccat ggcgaaaaag ttcgcttcga acttcggctg gcggttgcgc ttctggctgc
      181 tggggctgcc ggcgggcatc gggcttgcca cgctgcgcgc gatcctcaag ctgtggatcg
      241 gctttcccgc gcgctggagc ggcgtgttct cggcaggcaa cgggcccgcg atgcgggtcg
      301 ctctgatcgg agtgtgtcac ggagacgatg ttccccggat gcgcgcgctg gtgcgcgccg
      361 cgacgcgcgt cacgcacacc gaccccaggg ccgagcacgg cgctctggcc gtggcgctcg
      421 ccgctcacct cgcctcgacc ggcaaggatg tcgcggctgg ggactacgcg cgcaaacttc
      481 gcgagcttct gggagacgac gggcgcgagc ttctggaatt ggtggacgat gtcgcgcaaa
      541 gcgtgggccg caaggaaacc gccgccgatt tcgcagcccg catcggctgc gcgaccggcg
      601 tcaccggcta catgctacac accgtgcccg ccgcgctcca cgtatggctg cgccgacaga
      661 ccgactatcg ccctgcactg atcgaaacgg tgcggctggg tggagacacc gacactgtcg
      721 cggcggtagt cggcgcgctg atcggcgcgc gcgttggcaa ggaaggaatc cctaccgagt
      781 ggctgcgcga cctgtgggaa tggccgcgca cgaagacgtg gttggatcag ctcagcgtta
      841 ggctcgcgga acgctgcaag gaccacgcga gcggcggcgc cgtttcgctc aactgggtgg
      901 cgctgctgct gcgcaacgcg cttttcatcc cgctcgtgct tgcgcacggc ttccggcggc
      961 tgctcccgcc gtactgaagg aggcgtgata agtgacgagt gataagtgat aagagacccg
     1021 cgctcgcgcg gcttctttca cccgtcacct atcacttatc acctatcacc ctcaagctgc
     1081 aggacgccgc tcgcggcgct tactgcagct tgatgttgcc cttgcggatg acctccgcgt
     1141 acttgacgat ttcgcgcttg atctgggcag cgagctcttc cggcgtgctc ccgaccgggt
     1201 cgagcccttc cttcaccatg aattcgcgga cctttccgtt cttcagcgcg ttcacgatta
     1261 acccgttcat cttcgtcacg atgtcccggg gcgtattggc cggaaaccac atggcgtacc
     1321 agttatcgga ctcgaaatcc gggaagaacg tgttcatcgt gggcaggtcc ggaaacgctg
     1381 aagccttatt cgccgtggtc accgccagcg ccctgacccg ccccgacttg agcgccgccg
     1441 aggcgaccgg ccccgtcgca aagaggtagt cgatttcgcc gctcatcagc gccgcgatcg
     1501 ccggcccccc gcccttgtag ggtacgatga tcgcctcgat gcccgccctt tgcttcagca
     1561 tctcggccga aagatggctg gtgctgccgg ggacgttgat accggagttg agaaaagcgg
     1621 gtttctgctt ggcgagcgcg atcagctcct tcaccgagtt agcgggcacg ctcgggtgca
     1681 cgaccagcac cagcggcgtg gacgtgagcc agctcaccgg cgcgaagtcc ttcaccggat
     1741 cggtcttcat ccggctgccg tagagcgtgg tgttgatcgt gagcgtggcc gtggagaaca
     1801 ggagggtata gccgtcgggc ggcgagttgg cggcgatttc cgccccgatc aacccgctcg
     1861 cgccggtgcg gttctcgatc acgaaagtct ggccagtcgc ctcgtgaaac accgtcgtca
     1921 gcacccgcgc ctggatgtca gtgccgccgc cggggccaaa cggcacgatg atgcgcaccg
     1981 gcttgcccgg ccatgcctgg gcgaatgcgc aagcagaaaa caacattgac ccgcccgcca
     2041 gaacggcggc gaggatacgc aatgacaggc cctcactcat ggaaccctcc tcgctttgtt
     2101 ggaatgatgg tgatgcaggg atatggttcc atgtattgga ctcaattgca acccttagat
     2161 tcttaaccta aaaaatgctt caatttcagg acttcaatgt cgggactgga ccccttgccg
     2221 aaaaaaacgg ggccagcagg ccccgttctc cagttgcttg gcaagcgacg cttaatcgac
     2281 tttgatgttg cccgccgtga tgatcttcgc gtagcgagcg atctcgcgct tgaagttggc
     2341 gtgcaattcc tccgggctgc tcgcgaccgg atcgagtgcc tcccgcggca tgaattccag
     2401 gatgtccttc gccttcagag ccttggcgat ctcggcgttg agcttgtcga taatcggcct
     2461 gggcgtgcct gccgggaaaa acatcgcgta ccagttatcc gactcgaagc cgggataaat
     2521 ggaggtcatc gtcggcagtt cgggaaaagc cgacgacttc tttgccgtcg tcaccgccag
     2581 cgccttgacc ttgcccgcct tgatctgggg cgtggctacc ggccccgtgc cgaacaggag
     2641 gtccgtttcg ccgctcacga gcgccaccat cgaaggcccg ccgcccttgt agggaatgtg
     2701 cgtcactttg acccccgtca tctgtctgag catctcgacc gacagatggc tcgtggtccc
     2761 ggcgccgttc gagcccgcgt tgagcgtgcc gctcttcttg gccagcgcca ccagctcctt
     2821 taccgatttc accggcacgc tcggatgcgt gaccagcacg agcggcgtgg atgaaatcca
     2881 gctaacgggc gccaggtcct tgagcgggtc gaacttgatc cgggtgccgt agagggtcac
     2941 gtttactgcc agcgacgcgg tcgtgaacaa tatcgtgtag ccgtcggccg gggcacgtgc
     3001 ggtgagctcg gcgccgatga tgccgcctgc accggagcgg ttctcgacga tgaacgtctg
     3061 ccccatgctc tcgaagaaat tcttgctgag gagcctggcc tggatgtcgg tgccgccccc
     3121 gggaccgaat ggcacgatga tgcgcaccgg cttgaccggc caggcctggg cgtaagccgc
     3181 gcctccaagc gcgaaaccca cagcgacggc gacaagttgc ggcaataggc ttctgttcat
     3241 tgctctctcc tcgtgtcgta ttgagttcta ttttggccga tgcttccgtt cgcccggtta
     3301 accgcgatat atcggctcgg gctgagtgaa gaacgaatgc aggatatggt aaaccatcag
     3361 gtagttgatc tgctggaagc tcgcgtgcgc gatgcccgcc atcacggcga actgcttgtc
     3421 gggattgggc aggcgcttga aaaactcagt gaggtcgtcg aagccggcaa tgccgtcgaa
     3481 ctgcccgcgc atgatgaggg tcggcaccgt gattttcgcg ggatccacca ccggcagctt
     3541 cgagcacatg tccacgtagg ttccggtggg caccgagtcg tcgagcttga ggatgtgatc
     3601 ggcgaacgcc tcgatcacct tgtcctcggc gcttcccggg tgatcgcgat tgaagatgct
     3661 gcgcacgaag gcgcggtcga tcgggcggcg gttcttcgcc tggaactcgg gcagcttctt
     3721 gcgccgctcc gcgagcgtgg ggctgccctc cccggtccac acgaaggcgt cgagcgcgag
     3781 gcgcttcacg cgcccggggt gccgctgcgc gaacagcgcg gcgcgcaacg cccccgatga
     3841 aatgccgtag acgagaagct gccgggtccc gcgcgtcttc atgatgtaat cggttgccgc
     3901 ctcgagatcg tcggcgccgt tcgcgatgtc gcagttgatg tcgcgcttct tgtccgagcg
     3961 cccgtagcct tccatgtcca cgcaccacgc gtcgaaaccg cgctcggcga agaaatccat
     4021 cgcggacgaa tgcgggcgtc ccggcacctg gagatcgaag gtgggctggg agcccatcga
     4081 ggacccgtgc acgaacagta tcgttccccg cggcttggcg gcgcgctttt cccacaggaa
     4141 gagcctgacc tcccccttgt gggtccagtg ttcctgtccg gtgattttcg ctgcggcttt
     4201 cggctgatcc acgatgacct ctttgaacgg ggatgactcg gcgaaggaag gcgaagtcta
     4261 ggatacggac ccggcagcgt caatttcggt ttgctgacga agtaagttgg gccatactca
     4321 gcactcagca cacagaaaat gacccggctc tacgaaaaag ctcgcatcgg cgacatcgac
     4381 atccactacc acctcgccga ctacaccgat ccctggcgca agccgcccgc ggaaaccttc
     4441 ctgtgttatt ccggctactg ccgcagcctc gagttctggc atgcgtgggt gccgctgctc
     4501 gcgcgcgatt accgtgtgct gcgcatggac ccgcgcggtt acggccacag cggcaagccc
     4561 ggtcccggat accggttcga gatcgaacag ctcgctcgcg acgcgctcgg actgatggac
     4621 cacctcggca tcgagcgcgc gcactgggtg ggggattcca ccggcggcgc ggtcggaatg
     4681 tccgcggcgc tcctcgcccc gcggcgcatc tcatcgatca cggcctgcaa caccaccgcg
     4741 aaaatggcgc agcagacggt ggacacctac gccatcggcg agcaggacca ggccgccgcg
     4801 atcgagaagt tcggtgtcta cgaatggtgc cggcggacgc tcaagcatcg ggtagacatc
     4861 tcgaagattc cgcccgagct cggcgaatgg tgggcgcgcg acatggaccg cacgccgcgc
     4921 cacgtggcga ccgccgtgtt tcgctgcttc tccaccgccg acctgtggcc gcggctgaag
     4981 gagatccggg cgccgacgct gctgatcgtc ggcgacaact gcccggaaat ccgcaagcgg
     5041 cagctcgccg agatggcgaa aacgctgccg cggggcaagc tcgtgaatct cgagggttac
     5101 gactacggca tccacttcct cgcgcccgag cggtgtgtc
//
LOCUS       DBNGWZ010000420        11004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2193036, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000420 DBNGWZ010000000
VERSION     DBNGWZ010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11004
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2193036"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..940
                     /gene="rfbD"
                     /locus_tag="AC16CX_12675"
     CDS             26..940
                     /gene="rfbD"
                     /locus_tag="AC16CX_12675"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR3013402.1"
                     /translation="MGTILLTGRDGQVGHELVTALAPLGRLVATSRAELDLSSERSVR
                     AAMRDIAPDIIVNAAAYTAVDKAESEEELAMLVNGAAPRVIAEEAERLGSLLIHYSTD
                     YVFDGTSTQPYTEEDRPNPLNAYGRSKLAGEEAIAATGAAHLILRTSWVYSARGSNFL
                     LTILKLVRERKQLRVVDDQIGSPTWARSLAQATARLLADGERCRANTGIYHLSASGST
                     SRFEFVRAIIEISREKLGPKEGWAELRPISSAEYPLPAARPLNSATSKEKIKRVFGIE
                     MPPWRRQLEDCLGDPAARDAFRRVMQGG"
     gene            975..1151
                     /locus_tag="AC16CX_12680"
                     /pseudo
     CDS             975..1151
                     /locus_tag="AC16CX_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
     gene            1310..2443
                     /locus_tag="AC16CX_12685"
     CDS             1310..2443
                     /locus_tag="AC16CX_12685"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3013403.1"
                     /translation="MTPPKGSFFLFGVRGAGKSTWAREQFPDAYVVDLLEESRYQALL
                     ADPGLLALELRGLPAQRTVVLDEVQRVPALLNEVHRAIESARRRFVLLGSSARRFKTA
                     ATNLLAGRATLRTLYPLVPAELGQDFDLERVLRFGSISLVWQADDPRATLEAYVQLYV
                     REEIRAEALVRNLPGFLRFLPVAALFHGQVVNVAGLARDAATARTTVEGYLGILQDTL
                     LATLLPAFEPRLRMRERRHPKLYWVDPGLVRAAKRQLGPVGAEERGLLLEGWILTVLL
                     AHNERSALFEEVTYWAPLQARQTEVDFLLRRGREYLALEVKAQPRFSSPQLSGLRAIA
                     DLPRIARRVLVYLGERRLRTDDGIEVWPLDFFLEKLAGGRLWP"
     gene            2774..2977
                     /locus_tag="AC16CX_12690"
     CDS             2774..2977
                     /locus_tag="AC16CX_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013404.1"
                     /translation="MNSQGEESILEELKGLPPERVAEVEDFVDFLRVRAEEQRLVKSA
                     TKASEPAFRKIWDNPEDADYDRL"
     gene            2989..3135
                     /locus_tag="AC16CX_12695"
     CDS             2989..3135
                     /locus_tag="AC16CX_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013405.1"
                     /translation="MPTYSFGDVIFVPFPFTDQTGTKKRPAVIVSNAAYKSARSCADV
                     PTNI"
     gene            3211..3438
                     /locus_tag="AC16CX_12700"
     CDS             3211..3438
                     /locus_tag="AC16CX_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR3013406.1"
                     /translation="MNYRNRITIEPGKRGGRPCIRSMRITVYDVLGWLASGMTEQEIL
                     RDFPELEQEDIRACLEFAADREHHLISVRDT"
     gene            3444..3767
                     /locus_tag="AC16CX_12705"
     CDS             3444..3767
                     /locus_tag="AC16CX_12705"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR3013407.1"
                     /translation="MLDQNISYRLLDKLDATYPGSAQVRLAGMAAASDAALWQYAKEN
                     GFVIVTKDSDFHELGLVRGFPPKVIWLQCGNTSSQYILNILLNNHKAVQNFIKDTENA
                     CLELY"
     gene            4223..4573
                     /locus_tag="AC16CX_12710"
     CDS             4223..4573
                     /locus_tag="AC16CX_12710"
                     /inference="COORDINATES: protein motif:HMM:NF027249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HIR3013408.1"
                     /translation="MRYPIEVFWSEEDEGYIAIAPDLPGASAWGKTEVEAINELHLAI
                     DLWIKAAKKVGNSIPKPSDRADVNYSSKFLMRVPKRLHSELAHAAKAQGVSLNQYVLY
                     LLTKGRGERGKRAA"
     gene            4769..6100
                     /locus_tag="AC16CX_12715"
     CDS             4769..6100
                     /locus_tag="AC16CX_12715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR3013409.1"
                     /translation="MKLTIIGTGYVGLVTGACFAEMGNSVTCVDTDAAKIEGLKRGRL
                     PIYEPGLEPLVISNVSEERLRFITSLAEAAADCSVYFIAVGTPPGEDGSADLTHVLDV
                     ARELGKLIAKDCIVVGKSTGPVGTAEQVRATIRSELDRRGVKIRLDVVSNPEFLKEGD
                     AVNDFMRPDRVIVGADSERAVEIMRTLYAPYIRNHERLMVMGVRDAEMTKYAANAMLA
                     TRISFMNEMALLCEKLGIDVENVRRGIGSDSRIGHSFIYAGCGYGGSCFPKDIQALIH
                     IAEGAGVTPGILRAVEDRNRAQKERLFEKIHGRFGGRLKGLAFAVWGLAFKPGTDDIR
                     EASSLTLIERLLAAGASVKAYDPVAMDNARRSLPRDWFSPGKLSLVAHQYDAVAGADA
                     LALVTEWKPFRFPDFSAMKKAMKQPVIFDGRNQYDPQQVRAAGFEYFGIGR"
     gene            6097..6657
                     /locus_tag="AC16CX_12720"
     CDS             6097..6657
                     /locus_tag="AC16CX_12720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3013410.1"
                     /translation="MTVATFDHTATKWIFPPFRPGFVGRHYLIRRTSWVYAGRGINFV
                     LTVLRLAREKPELPMVNDQIGSPTWARALAEVTAELLKKKALREQGGTYHLSASGAAT
                     RYEFANEILTLAQELSEQTAGWATLRPILTAEYPLPARRPLTCITSKEKIKRVFGIEM
                     PDWRSQLRSCLAEFFASVEWQQRLRA"
     gene            6730..7707
                     /locus_tag="AC16CX_12725"
     CDS             6730..7707
                     /locus_tag="AC16CX_12725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3013411.1"
                     /translation="MPEPIASHKASVAIIIVNYNAGELLKRCLEALARQTLRPRRIYV
                     VDNASQDSSCDSIGSDFPEVDLLRLDRNAGFAAANNLAVRRAEECEWVALLNPDAFPE
                     PAWLERLMAAAAGHPEYHFFGSRMLMADTPELLDGAGDAYHVSGLVWREGHGVPANGA
                     YPEPREIFSPCAAAALYHRDEFLEAGGFDEDYFCYVEDVDLGFRLRLRGHRCLYVPDA
                     IVLHKGSALTGRRSDFSIYHGHRNLTWTFVKNFPSWWFWVALPLHLCACAFSLVWFSL
                     RGNSAAIWKAKIDAAKRVPEAWRKRRQIQAGRTAKPASVISLMTFALWP"
     gene            complement(7771..8748)
                     /locus_tag="AC16CX_12730"
     CDS             complement(7771..8748)
                     /locus_tag="AC16CX_12730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013412.1"
                     /translation="MLYENLTDRLFGTEGRWDIRACGNATCGLLWLDPMPIPEDIAKA
                     YADYYTHEDAPERSGHPLYRIFVAAKEGYLAAHYGYFAQGLSAWKRLAGWLLTLHPGR
                     RAVVDFSVMWLSALPGSRLLDVGCGSGWLLETMQRLGWAVEGVDLDPEAVKAARTKGL
                     RVHQGTLAGQQLPAGSFDAVTMSHFIEHVHDPGATLAECRRLLKHGGRLVVVTPNTES
                     LGHRLYGRSWMHLDPPRHLHLFNPDTLSEVVRRAGFGNVRAWTSLRDAYGIFLGSRSI
                     RRHGAYVMGSPQGGLIRLWARSAELVEWLALKFNSRLGEEITLIAEKTG"
     gene            complement(8813..10342)
                     /locus_tag="AC16CX_12735"
     CDS             complement(8813..10342)
                     /locus_tag="AC16CX_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR3013413.1"
                     /translation="MSETQFQPPMSHARLVRNSLFNVAGQGLPLLAAIVAFPWLIHGL
                     GADRFGVLALAWVLVGYAGLFDFGLGRALTQLVAQRLGSGHANDVPALAWTALAVMSA
                     AGVAVAAILMVASTWLVTRGLRIPADLQAESLEAVLILSISIPFVTCSAGLRGLLEAK
                     QQFGPVNVIRGSLGILTFLGPVAVLFASNHLGAIMWVLVTIRIAAWAASLMLCVNSFP
                     ELRSGLTFRRNAIPVLIATGGWITVSNVVSPVITYLDRFLIGALVSTVAVAYYVTPYE
                     VITKVVIFPLAFATALFPEFAVKAASDARQATILYVSSLKYVFVLLFPIVLVAVAFTP
                     EALALWLGSDFSKNSSTVLRVLAAGVLLNGLAQVPFALTQAAGRADWTAKLHLAELIV
                     YVPVLYAAIAAYGIVGAATAWSARVAIDLLALAGMARSLLSPHPLHTRSVWLWAGVTG
                     VALAGAWLVQGLAAKALFLGAVLTAFALVSWRVWLSAEEKTGLMTWFARSPESQDRSG
                     H"
     gene            complement(10339..>11004)
                     /locus_tag="AC16CX_12740"
     CDS             complement(10339..>11004)
                     /locus_tag="AC16CX_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3013414.1"
                     /translation="GPLESLSRECLKDISGCRAEVVSGHGNLGYGRGHNLAIARSSAE
                     YHLIVNPDVIVDAQALVEAVRFMEAYPDVGLLAPAVRGEDGATEHLCKRYPSVLDLAL
                     RGFARESVKRAFRERLERYEMQAETGESVHLDVPIASGAFMFCRGRVLRLVGGFSEAF
                     FLYFEDFDLSMRLRRIAKIAYVPAVKIVHLGGYAAEKGLKHMWMFTLSGIRFFNRHGW
                     RWW"
BASE COUNT         2118 a         3495 c         3440 g         1951 t
ORIGIN      
        1 gctgcggacg gccgaaacgt tttgaatggg cacgatactt ctgacgggta gggacggcca
       61 ggtcgggcac gagctcgtga cagcgctggc gccattgggc cggctcgtcg ccaccagccg
      121 cgccgagctg gacctgtcga gcgagcgatc ggtgcgcgcc gcgatgcgcg acatagcgcc
      181 ggacatcatc gtcaacgcgg cggcatacac cgcagtggac aaggcggaat cggaggagga
      241 actcgcgatg ctcgtgaacg gggcagcgcc gcgcgtcata gccgaggaag ccgagcgcct
      301 cgggtcgctg ctcatccact attccactga ttacgtgttc gatggaacaa gcacgcagcc
      361 ttacaccgaa gaggaccggc ccaatccgct caacgcctac ggccgatcca agctcgccgg
      421 cgaggaggcg atcgccgcga cgggcgccgc ccacctgatc ctgagaacga gctgggtcta
      481 cagcgcgcgc ggcagcaatt ttcttctgac catcctcaag ctcgtccggg agcgcaagca
      541 gttgcgcgtc gtggacgacc agatcggctc gcccacctgg gcgcgctccc tcgcgcaggc
      601 gaccgcccgc ctcctcgccg acggcgagcg ctgccgggcg aacaccggca tctaccatct
      661 gtcggcgagc ggctccacct cgcgcttcga gttcgtgcgg gcgattatcg agatctcgcg
      721 ggaaaagctg ggaccgaagg aaggatgggc cgagttgcgg ccgatctcga gcgcagagta
      781 ccccttgccg gccgcgcgcc cgctcaactc ggccaccagc aaggagaaga tcaagcgcgt
      841 cttcggcatc gagatgccgc cctggcggcg gcaactggag gactgtctcg gcgatcctgc
      901 cgcgcgcgac gctttccggc gcgtgatgca gggaggatga tcgtcagatg aaattgctgg
      961 tcatcggcgc cgccggcttc ggcaacgtgc tcgagggcgc gcggcgctcc ggcgttagac
     1021 acctcgtctt tgcatcctcg agctcgatct acggcgcgaa cgcaagactg ccgttctccg
     1081 agcatgacaa cgtggaccat ccggtgttgc gctatgccgc caccaagaag gcgaacgagc
     1141 tgatggcgca ctgggagcac ccgctgcggg actttctcgc cgggttgagc ggcacagcgc
     1201 cgcccgcgga ggccggccgc tgaaccgtgg gcggccggga cgttagaagg atcgccgtca
     1261 ttttgcgtgg taaaatgacg acatacctgg cattcattat cgccgcctga tgacgccgcc
     1321 gaagggaagc ttcttcctgt tcggcgttcg cggcgcgggc aagagcacct gggcgcggga
     1381 gcagtttccc gatgcctacg tcgtcgatct cctcgaagaa tcgcgttatc aggcgctcct
     1441 cgccgatccc ggtctgctcg ccctggagct gcgcggcctg ccggcgcaac gcaccgtcgt
     1501 gctcgacgaa gtgcagcgtg tgcccgccct gctgaacgag gtgcaccggg ccatcgagag
     1561 cgcgcgccgc cggttcgtgc tgctcggatc cagcgcgcgc cggttcaaga ctgcggccac
     1621 caatcttctc gcggggcgcg cgacgctgcg aacactgtat ccgctggtac cggcggagct
     1681 gggccaggac ttcgacctcg aacgggtgct gcgctttggc agcatttcgc tcgtgtggca
     1741 ggccgatgat ccgcgcgcga cgctcgaggc ctacgtgcaa ctctacgtgc gcgaggaaat
     1801 ccgcgccgaa gctctcgtca gaaacctgcc gggattcctg cggttcctgc ccgtggcggc
     1861 gctgtttcac gggcaggtcg tcaacgtggc cgggttggcg cgcgatgcgg cgaccgcgcg
     1921 caccaccgtc gagggctacc tcggcatcct ccaggacacg ttgctcgcga cgctgttgcc
     1981 cgccttcgag ccgcgcctgc gcatgcgcga gcgccggcat cccaagcttt actgggtcga
     2041 tcccggcctc gtgcgggcgg cgaagcggca actgggtccg gtgggtgccg aagagcgcgg
     2101 cctgctgctg gaaggttgga tccttacggt gctgctggcc cacaatgagc gatccgccct
     2161 gttcgaagag gtgacgtatt gggcgcccct gcaggcccgc cagaccgagg tcgatttcct
     2221 gctgcggcgt ggccgggaat accttgcgct cgaggtcaag gcgcagcccc gtttctcgtc
     2281 gccgcagctc tccgggttgc gggcgatcgc ggaccttccg cgcatcgcgc gccgcgtgct
     2341 ggtctatctg ggcgagcgcc ggttgcgcac tgacgacggc atcgaggtat ggccgctcga
     2401 ttttttcctg gagaaactcg ccggtggccg tctctggccg tgagacaatc gcgtaagatc
     2461 tcagccacgg aaacgcacgg aaggtcacgg aagtcttctg aatgattttc gatctgaatc
     2521 cttttccgtg catttccgtg tgattccgtg gctaatggtt ttgccgattc acgatttacg
     2581 attcacaggt tctgtccgac tcacgattta cgattcacgg ccctttcccg attcaggagt
     2641 catggattac ggggtttaac cagccactag ccacaagccg cggcatccgc ttccctaagg
     2701 tagtctggaa ctcgcgtgag aatacgggat aactgggaag ctggaccgct tacccgcaga
     2761 aggagcaacc accgtgaaca gccagggcga agaaagcatt cttgaagagc tcaagggcct
     2821 accgcccgag cgagtggcgg aggtggagga ctttgtcgat ttcctgcgtg tgcgcgccga
     2881 ggagcaacgc ctcgtgaaat ccgcaacaaa ggcctcggag cctgcgttca ggaagatctg
     2941 ggataacccc gaggatgccg actacgatcg gctatagacg gggtaatgat gccgacctac
     3001 agcttcgggg acgtcatctt cgtacctttt ccgttcaccg accagaccgg aaccaagaag
     3061 cgtcccgctg tcatcgtcag taacgcggcc tacaaatcgg cgcggtcttg tgctgacgtg
     3121 ccgacgaaca tctgaggcta cgacccacga accacgataa gcgtataatt tatcgagtca
     3181 atcttgttgt tgccctgaat agggataggt atgaattatc gtaaccgcat taccatagag
     3241 ccggggaagc gaggcggcag accttgcatt cgttccatgc gaatcaccgt ctatgatgtg
     3301 ctggggtggt tggcctctgg catgacggag caggaaatcc tgcgcgactt tccggaactg
     3361 gaacaggagg atattcgtgc ctgccttgaa tttgccgccg accgcgaaca tcatctcatc
     3421 agtgtccgcg acacttgaag ctcttgctgg accagaatat ctcttatcgg ctgctcgaca
     3481 agcttgatgc gacctatcct ggttccgcgc aggtacggct tgccggtatg gcagcggctt
     3541 cggatgcggc gctttggcaa tatgccaagg aaaacggttt tgtcatcgtc accaaggact
     3601 cggacttcca tgaacttggc ttggtgagag ggtttccccc gaaagtgatc tggcttcagt
     3661 gcggtaacac atcctcgcag tacatcctga acatcctctt gaataaccac aaggcggttc
     3721 agaatttcat aaaagacacg gaaaacgcat gtttggaatt atattgacca aacccgtgcc
     3781 acgagccacg gctcacggat tctgtcgaat cacgactcac cggtttaatt cactcaccac
     3841 tcaccgctca cgactcaccg tttcttcgtc ggctcacggc tcacggggtt tcggatcacg
     3901 attcacgatc tccgattcac gattcacggc ctttgaccac cagccacgat ccacgacccg
     3961 ctcgccgctt gactctgcta tatcgatgcc ctttgggagc ggatcggaca aggcctgaaa
     4021 tccgcggggg tgggtgtcga agacgtggca aaggcggtac gcgaagtcag gaaatcaaaa
     4081 gcacggaatg cggctcgtcg ttgacacgaa cggctcgtga cttgtgacgg gtagctggtc
     4141 acccgcaagt ggattgatac catatgtagt aatcaggccc ggcagttgat tgacatgttg
     4201 gataaatatg agggttgaca tcatgcgcta tccgatcgag gttttctgga gcgaggaaga
     4261 cgaaggctat atcgccattg cccccgacct tcctggcgcg tcggcctggg gcaagaccga
     4321 ggtcgaagcg atcaatgagc tgcatcttgc gatcgatctc tggatcaagg cggccaagaa
     4381 agtgggaaac tccattccca agcccagcga ccgcgccgac gtcaattaca gtagcaagtt
     4441 cctgatgcgt gtaccgaagc gactgcattc ggaacttgcg catgctgcca aggcgcaagg
     4501 cgtcagcctc aaccagtacg ttctctatct cttgacaaaa ggccgcggcg agcgtggcaa
     4561 acgcgcggcc tgaacctccg tgagtggtga catcctcagc gggctggcac ggcgtaaact
     4621 cttgcggtca cctgcatcat tccccgcttc cgcaagcggt ccattacaag tgcctcattg
     4681 ccgcaggggc gcgcagatca gcgggttcta tgagaaaatt ctccgctcct gaagaataag
     4741 cggtccattt cagaggaaat atccgggctt gaagctcacc atcataggca ccggctacgt
     4801 cgggctcgtc accggcgcgt gcttcgccga gatgggcaac tcggtgacct gcgttgacac
     4861 cgatgccgcg aagatcgagg gcttgaagcg ggggcggctg ccgatctacg agcccggcct
     4921 cgagccgctc gtgatttcca atgtgagcga ggaaagattg cgtttcatca cttcgctcgc
     4981 cgaagcggcg gcggattgca gcgtttattt catcgccgtc ggcacgcccc cgggcgagga
     5041 cggctcggcg gatttgacgc acgtgctcga cgtggcgcgg gagctgggga agctcatcgc
     5101 gaaggactgc atcgtggtcg ggaaatcaac cggccccgtc ggcaccgccg agcaggtgcg
     5161 cgcgacgatc cgctcggagc tcgaccggcg cggcgtgaag atccgtctgg acgtggtgag
     5221 caacccggag ttcctcaagg aaggcgacgc ggtcaatgat ttcatgcggc ccgaccgggt
     5281 gatcgtggga gccgattccg aacgagcggt ggagatcatg cggacgctct acgcgccata
     5341 catccgcaac cacgagcggc tgatggtgat gggggtgcgc gacgccgaga tgacgaagta
     5401 cgcggcgaat gccatgctcg cgacgcgcat ctccttcatg aacgagatgg cgctcctctg
     5461 tgagaagctg ggcatagacg tggagaacgt gcgccgcggc atcggctccg acagccgcat
     5521 cggacactcc ttcatctacg cgggatgcgg ctacggcggc tcctgttttc ccaaggacat
     5581 ccaggcgctg atccatatcg ccgagggcgc cggcgtcacc cccggcatcc tgcgcgccgt
     5641 ggaagaccgc aaccgggcgc agaaggaacg cctgttcgag aagatccacg gccgcttcgg
     5701 cgggcggctc aaggggcttg cgtttgccgt gtggggactc gccttcaagc cgggaacgga
     5761 cgatatccgc gaggcgtcat cgctcaccct gatcgagcgg ctgctcgcgg cgggcgccag
     5821 cgtcaaggcc tacgacccgg tggcgatgga caatgcgcgg cgcagcctgc cccgcgattg
     5881 gttctctccc ggaaaactct ccctggtggc tcatcagtac gatgcggtcg cgggcgcgga
     5941 cgccctggcg ctggtcaccg agtggaagcc gttccgattc cccgacttca gcgccatgaa
     6001 aaaggcgatg aagcagccgg tgatcttcga cggtcgcaac cagtacgacc cgcagcaggt
     6061 acgcgcggcg gggttcgagt atttcgggat cgggcgatga ccgtggccac tttcgatcac
     6121 accgcaacca agtggatctt cccacccttc cgtccgggtt tcgtcggcag gcactacctg
     6181 atccgacgca cgagctgggt gtacgcgggc cgcggcatca acttcgtcct gacagtgttg
     6241 cggctggccc gtgagaaacc ggagctgccg atggtgaacg accagatcgg ctcgccgacc
     6301 tgggcgcgcg ctctggccga ggtcaccgcc gagctgctca agaagaaggc gctgcgcgag
     6361 cagggtggca cctatcacct gtcggcgagc ggggcggcca cacggtatga attcgcgaat
     6421 gagattctca ctttagccca ggagctttcc gagcagaccg cgggctgggc gacgctccgc
     6481 ccgatcctca cggccgagta tccgttaccc gcccggcggc cgctcacctg catcacgagc
     6541 aaggagaaga tcaagcgcgt gtttggcatc gagatgccgg actggcggag tcagttgagg
     6601 tcctgcctgg ccgagttttt tgcgagcgtc gaatggcagc aacgattgcg cgcctgagcc
     6661 tggaatccag gggaattggt ctcccaggtc gattcttcgc agcatcacca cctattcacc
     6721 gagtccgccg tgcctgagcc gattgcatcg cacaaggcca gcgtcgcgat catcatcgtc
     6781 aactacaacg cgggcgagct cttgaagcgc tgccttgaag cgctcgcgcg ccagacgctc
     6841 cggccgcgcc gcatctacgt cgtggacaac gcgagccagg acagttcatg cgacagtatc
     6901 ggcagcgatt tcccggaagt cgatctcctc cggctcgatc ggaacgcggg gtttgccgca
     6961 gccaacaacc tcgctgtgcg ccgcgccgag gaatgcgagt gggttgcgct gctcaatccc
     7021 gacgcgttcc cggaaccggc gtggcttgag cggctgatgg cagcggccgc cgggcaccct
     7081 gagtaccatt tcttcggctc gcgcatgttg atggctgaca cgccggagct gctcgatggg
     7141 gcgggcgacg cctaccacgt gagcgggctg gtatggcgtg aaggacacgg tgtgccggcc
     7201 aatggcgcct atcccgagcc acgtgagatt ttctcgccgt gtgcggcggc cgcgctttat
     7261 caccgcgacg agtttctgga agcgggcggc ttcgacgagg actacttctg ctacgtggaa
     7321 gacgtcgacc ttggtttccg cctgcgcctg cggggacacc gttgcctgta cgtgccggac
     7381 gcgatcgtcc tacacaaggg atccgcgctc accggccgcc gtagcgactt ctcgatctat
     7441 cacgggcacc gcaatctcac ctggacgttc gtgaagaact ttccgtcgtg gtggttctgg
     7501 gtcgcgctac cgctgcacct ttgcgcgtgc gctttcagcc tcgtctggtt ctcgctgcgc
     7561 ggaaatagcg ccgcaatctg gaaagccaag atcgacgccg cgaagcgcgt gcccgaggcg
     7621 tggcgcaagc ggaggcagat tcaagcgggc agaaccgcaa agcccgccag cgtgatctcg
     7681 ctcatgacat tcgccctgtg gccgtaacaa gttcacgctg atcgctcgat ggctacgcgg
     7741 cgccgagcaa gttctggcgt tgcgcaagat ctaacccgtt ttctcggcga tcaaggtgat
     7801 ctcctcgccg agacgcgagt tgaacttgag cgccagccac tcgaccagtt cggcgcttct
     7861 cgcccataat cggatgagcc cgccctgcgg cgagcccatg acgtaggcgc cgtgacgacg
     7921 gatcgagcgg ctgcccagaa aaattccgta ggcgtcgcgc aatgacgtcc acgccctgac
     7981 gttcccgaat cccgcgcgcc gcacgacctc ggacagcgtg tccgggttga acaggtgcaa
     8041 gtggcgcggc ggatcaaggt gcatccagct tcttccatag agccggtgtc cgaggctctc
     8101 ggtgttcggc gtgacgacga caagccgccc gccgtgcttc agcagccgcc ggcattccgc
     8161 gagcgtggcg cccgggtcat gcacgtgctc gatgaaatgg ctcatcgtca cggcgtcaaa
     8221 gcttccggcc gggagctgct gccccgccag ggtaccctgg tgcacgcgaa ggcctttcgt
     8281 acgcgcagct ttgaccgctt ccgggtcgag gtccacgccc tcaaccgccc agccgaggcg
     8341 ctgcatcgtc tctaacaacc acccgctgcc gcagcccacg tcgagcagcc gactacccgg
     8401 gagcgcgctc agccacatca cggaaaaatc cacgactgcc cgccgccccg gatggagggt
     8461 gagcagccaa ccggccaggc gcttccaggc ggacaggcct tgcgcgaaat agccgtaatg
     8521 cgctgcgagg tagccttctt ttgcggcgac gaatatgcgg tatagaggat ggcccgaccg
     8581 ctccggcgcg tcctcatggg tgtagtagtc ggcatacgcc ttggcgatgt cttccggaat
     8641 cggcatcgga tcgagccaca gcagcccgca ggtcgcgttt ccgcaggcgc gaatgtccca
     8701 cctgccttct gtcccgaaca gacggtcagt gagattttcg taaagcacct ttccggcgct
     8761 tcggcagaga tagcaatcgg ggcgggggtc ggagcggatc acgcgggata tcttagtgcc
     8821 cggaacggtc ctgactttcg ggagaccggg caaaccacgt catcaagccg gtcttttctt
     8881 ccgccgataa ccacacgcgc cagctcacga gcgcgaatgc ggtcagcacc gcgccgagaa
     8941 aaagcgcctt ggcggccagc ccctgcacca gccatgcgcc cgcgagggca acaccggtga
     9001 cacccgccca gagccagacg ctgcgggtgt gcaatggatg aggcgacaac aggctccgcg
     9061 ccattcccgc cagcgcgagc aggtcgattg cgacgcgggc gctccaggcc gtggccgccc
     9121 ccacgatgcc gtaggcagca atcgctgcat acaaaacggg aacgtagacg atcagctccg
     9181 caagatggag cttggccgtc cagtccgcgc gacctgcagc ctgggtcaag gcgaatggaa
     9241 cctgcgccag gccgttgagc agcacgcccg cggcaagcac gcgcaggacg gtcgagctat
     9301 tcttggagaa atccgaaccc agccaaagcg cgagcgcctc gggtgtgaac gcaaccgcca
     9361 cgagcacgat gggaaacaag agcacgaaca catacttcag cgatgagaca tacaggatgg
     9421 tcgcttgacg ggcatcgctc gccgccttca ccgcgaattc cgggaatagc gctgtcgcga
     9481 aggccaaggg aaagatcacc accttggtga tcacctcata aggagtgacg tagtacgcca
     9541 ccgccaccgt ggaaaccagt gcaccgatca ggaaccggtc aaggtaggta atgacaggac
     9601 tcaccacgtt cgacaccgtg atccagccgc cggtcgcgat cagtaccggg atcgcattgc
     9661 gccggaacgt gagccccgaa cgcagctccg ggaagctatt gacgcaaagc atcaggctcg
     9721 cagcccacgc tgcgatccgg atcgtcacca gcacccacat gatcgcgccg aggtggttgg
     9781 aggcgaagag taccgccacc ggccccagga acgtcaggat ccccagcgag ccgcggatga
     9841 cattgaccgg accgaactgt tgtttcgcct caagcagccc gcgcaggccc gcggagcagg
     9901 tgacgaatgg aatggaaatg ctcaggatca gcaccgcttc gagcgactcg gcttgcagat
     9961 cagcgggtat tctcaaccct cgcgtgacga gccacgtcga cgccaccatc aatatcgccg
    10021 ccaccgcaac gcccgcggca ctcatcaccg ccagcgcggt ccaggcgagc gccgggacgt
    10081 cattcgcgtg acccgaccct aatctttgtg cgacgagttg ggtgagagcc cgccccagcc
    10141 cgaaatcgaa caggccggcg tacccgacga gaacccaggc gagcgcgagc acaccgaagc
    10201 gatcggcccc gaggccgtgg atcaaccatg gaaaggcgac gatcgctgcg agcagcggca
    10261 atccctggcc ggccacgttg aagaggctgt tgcgaaccag cctcgcgtgg ctcatcggcg
    10321 gttggaactg cgtctcgctc accaccatct ccacccgtgc cggttgaaga acctgatccc
    10381 cgaaagggta aacatccaca tgtgcttgag acccttttcc gcggcgtagc cgccgaggtg
    10441 gacgattttc actgcgggca catacgcgat cttcgcgatc cggcgcaaac gcatgctgag
    10501 atcgaagtcc tcgaagtaga ggaagaacgc ttccgagaag ccgcccacca gccgcagcac
    10561 ccgtccccgg cagaacatga aggccccgct cgcgatgggc acgtcgaggt gaacgctctc
    10621 gcccgtctcc gcctgcatct cgtagcgctc caggcgctcg cgaaaggctc gcttcacgct
    10681 ctcccgcgcg aaccctctca gtgcgagatc caggaccgaa ggatagcgct tgcagagatg
    10741 ctccgtggcg ccgtcctcgc cccgcaccgc gggggccagc agtcccacgt ccgggtacgc
    10801 ctccatgaac cgcacggcct ctacgagcgc ttgcgcgtcc acgatcacgt cggggttcac
    10861 gatcaaatgg tattccgcgg acgagcgcgc gatcgcgagg ttgtggccgc ggccgtatcc
    10921 gagattgccg tgccctgaaa caacctctgc gcgacagcct gaaatgtcct tcaggcactc
    10981 gcgcgacaaa gactcaagtg ggcc
//
LOCUS       DBNGWZ010000421         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2193330, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000421 DBNGWZ010000000
VERSION     DBNGWZ010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2193330"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="AC16CX_12745"
     CDS             <1..316
                     /locus_tag="AC16CX_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR3013415.1"
                     /translation="SYEEKQFGTGEKKGQLRLIASPDGREGSVTIHQDAYVYASMLNG
                     GDAATHWLAPGRRAYLHVARGTVRANGTSLKGGDGVKIENEPSIELKDAREAEVLLFD
                     LP"
     gene            370..954
                     /locus_tag="AC16CX_12750"
     CDS             370..954
                     /locus_tag="AC16CX_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3013416.1"
                     /translation="MTKVAIVYHSGYGHTAEVAKAVARGAAGVDGIEAKLIRVEDVEK
                     HWQDLDEADAIIFGAPTYMGSVSAPFKAFMDASSKVWFKQGWKDKIAAGFTHSGSQSG
                     DKFNSLVQMFTFAMQHGMIWAGLGLMPGNNSSKGSLNDLNRLGGFGGSMSQSNVDQGA
                     EAILQSDLRTAEHLGKRVALVTLQFAGERALAAA"
     gene            1080..>1659
                     /locus_tag="AC16CX_12755"
     CDS             1080..>1659
                     /locus_tag="AC16CX_12755"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016701 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR3013417.1"
                     /translation="MTERQPAIFVSHGSPTLPLERGEAVNFLRGLGTALGRPEAILVV
                     SAHWDTTRPAVSAAEHPETIYDFYGFPPELYRLRYPAPGAPQLAPRVAELLQAGGLKA
                     DIDPERGLDHGAWTPLILMYSEADIPVTQLSIQSRLGPGHHVRLGEALRPLRDEGVLI
                     FASGGATHNLRELSRQRGNPLPQPWTVEFNEWL"
BASE COUNT          323 a          509 c          548 g          279 t
ORIGIN      
        1 gagctacgag gaaaagcagt tcgggacggg cgagaagaag ggacagctgc gcctgatcgc
       61 ctcgcccgac gggcgcgaag gctcggtcac gatccaccag gacgcgtacg tctatgcctc
      121 gatgctcaac ggcggggacg cggcgacgca ctggctggcg ccgggacgcc gcgcctatct
      181 gcacgttgca cgtggaacgg ttagggccaa tggcacatcg ctgaagggcg gcgatggcgt
      241 caagatcgag aacgagccct cgatcgagct gaaggatgcg cgggaagccg aagtgctgct
      301 ctttgacttg ccgtaaaggc gctcatcacc catcacccat tactcatcac acatctgaaa
      361 ggaacgaagg tgacaaaagt agcaatcgtg tatcacagcg gttacggcca tacggccgag
      421 gtggcaaagg cggtcgcgcg cggcgcggct ggcgtggatg gcatcgaagc caagctgatc
      481 cgggtcgaag acgtcgagaa acactggcag gacctcgacg aggccgacgc catcatcttc
      541 ggcgctccga cctacatggg cagcgtgtcc gccccgttca aggcgttcat ggatgcgagc
      601 tcgaaggtct ggttcaagca gggctggaag gacaagattg cagcgggctt cacgcattcc
      661 ggcagccaga gcggtgacaa attcaactcg ctggtgcaga tgttcacttt cgccatgcag
      721 cacgggatga tctgggcggg cctggggctg atgcccggca acaactcgag caagggcagt
      781 ctgaacgacc tcaaccgtct cggtggattc ggcggctcga tgtcgcagtc gaacgtggac
      841 cagggcgcgg aggcgatact gcagagcgat ctcaggaccg ccgagcatct cggcaagcgc
      901 gtagcgctgg ttacgctgca gttcgcgggc gagcgcgcgc tcgcggcggc ataacttcaa
      961 cctctaatcg ccaagggcgc caaggaacgc caaggaacgc caaggaaaca caatcaaggg
     1021 taggataacc cccctgattt taggtttcct ttgcgtcctt cgcgttgaga gtttgggtta
     1081 tgaccgaacg ccagcccgct atcttcgtct cgcacggctc gccgacgctg ccgctcgagc
     1141 gtggcgaagc cgttaatttc ttgcgcggtc tcggtacggc gctgggccgg ccggaagcaa
     1201 ttctcgtggt ttcggcgcac tgggacacca cgcgcccggc cgtaagcgcg gcggagcatc
     1261 cggaaacgat atacgacttc tacggcttcc cgcccgagct ctaccggctg cgctaccccg
     1321 cgccgggtgc gccgcagctg gcgccgcgcg tcgcggaact gctccaggcc ggcggactga
     1381 aggctgacat cgatccggag cgcggcctcg atcacggcgc gtggacgccg ctcatcctga
     1441 tgtactcgga agcggacatc ccggtcacgc agctctcaat ccagtcgcgg ctcggtcccg
     1501 gtcatcacgt gcgcctgggc gaagcgctgc ggccgttgcg cgacgagggc gtgctgatat
     1561 tcgcttccgg gggcgctacc cacaaccttc gcgagttgag ccgccagcgg ggcaatcctt
     1621 tgccgcagcc gtggaccgtc gagttcaatg aatggctgg
//
LOCUS       DBNGWZ010000422         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3200408, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000422 DBNGWZ010000000
VERSION     DBNGWZ010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3200408"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="AC16CX_12760"
     CDS             complement(<1..944)
                     /locus_tag="AC16CX_12760"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3013418.1"
                     /translation="MPPPRLLILASAHTYRLEPFLEAARKLGVEVVRGLDVPPTHVIS
                     KEDFLGLDFRDVERSLAQAQTYALQHRLRAVIPTDDATVGLAAHLSEALDLPHNSIEA
                     AEAARDKYRMRQLFAQAGLPTPWFRLASIASDPARLAAQVKYPCVLKPTCLAGSRGVI
                     RANDARQFAAAFARLKAILVRAGLNDVLVEGYIPGSEVALEGLLTHGNLKVLALFDKP
                     DPLEGPFFEETIYVTPSRLPPETQAAIHTSAAGAAGALGLREGPVHAELRLNDEGVWP
                     LEIAGRSIGGLCSQTLRFGTDLSLEELILRQALGEEIES"
     gene            complement(979..>1975)
                     /locus_tag="AC16CX_12765"
     CDS             complement(979..>1975)
                     /locus_tag="AC16CX_12765"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3013419.1"
                     /translation="KARSAGTAYNSRREEVIPLRGVRMPHITYQNEAVVEVDTTTPIL
                     QASLQHGIPHTHVCGGNARCSTCRVLILEGLDYCCPRNEKEQKMATRRNFSPMVRLAC
                     QTTVSGDVALRRLVLDDEDVQLVDQEMAGGATPRSVGEERHLAILFADVRNFTAFAEA
                     HLPYDVVHVLNRYFSRVGAIINHHHGQINNFIGDGLMALFDADDSTGPTLNAVRAGLE
                     MLRAVEAMQPYFEAQFKINFRIGIGVHYGEVVLGTVGSGDSRRLTAIGDTVNLASRIE
                     SANKEAGTEFLISEEAHQQVKDRVRIGKHCDLAIKGKTGTYSLHEVVALTDTDHD"
BASE COUNT          335 a          633 c          649 g          358 t
ORIGIN      
        1 gattcgatct cctcccccag cgcctgccgc aagatcaatt cctccagcga caaatccgtc
       61 ccgaagcgca gcgtctgcga gcacagcccg ccgatggagc ggcccgcaat ttcgagcggc
      121 cagacacctt cgtcgttgag ccgcagctcg gcatgcaccg gcccttcgcg caaaccgagc
      181 gcgccggcgg ccccggcggc gctcgtgtgg atggccgctt gcgtctccgg cggcaggcgc
      241 gagggtgtca cgtaaatggt ctcctcgaag aacggccctt cgagcgggtc gggcttgtcg
      301 aacagcgcca acactttcaa gttgccgtgc gtcaggaggc cctccagcgc gacctcgctg
      361 cccggaatat aaccttccac caacacgtcg ttcagcccgg cccggacgag gatcgccttg
      421 aggcgcgcaa acgcggcggc gaactgccgt gcgtcgttgg cgcggatcac gccccggctg
      481 ccggcgaggc acgtcggttt gagcacgcac ggatatttca cctgcgcggc aagtcgggcc
      541 gggtcgctgg cgatggaggc gaggcggaac cacggggtgg gcaatcctgc ttgcgcgaag
      601 agctgccgca tgcggtattt gtcgcgggct gcttccgcgg cttcaataga attatgcggc
      661 aggtcgagcg cctcgctcag gtgcgcggcc agccccaccg tcgcatcgtc ggtggggatc
      721 accgcccgta accgatgctg caaggcatac gtctgcgcct gcgcgaggga gcgctcgacg
      781 tcgcggaaat ccagcccgag gaaatcctct ttggaaatta catgggtcgg gggaacgtcg
      841 aggccgcgga cgacttcaac tcccagcttg cgcgccgcct ccagaaacgg ctcaagccgg
      901 tacgtgtgcg ccgaggcgag gatgagtaag cgtggagggg gcatcggagt gggctgatca
      961 ccgatgacgg atgacggctc aatcgtggtc agtatcagtc aatgccacta cttcgtgcag
     1021 cgagtacgtg ccggttttgc ccttgatggc gaggtcgcaa tgcttgccga tgcgcacgcg
     1081 gtctttgact tgctggtgcg cctcctcgga gatgaggaac tcggtcccgg cctccttgtt
     1141 ggcgctctcg atgcggctgg cgaggttcac ggtgtcgccg atggcggtga gccgccggct
     1201 gtcgccggag ccgacggtcc cgaggaccac ttcgccgtaa tggacaccga tgccgatacg
     1261 gaagttgatt ttgaactgcg cctcgaaata gggctgcatg gcctctacgg cccggagcat
     1321 ctccaacccg gcgcggacgg cattgagcgt cgggccggtg gaatcgtcgg cgtcaaagag
     1381 cgccatcaac ccgtcgccga tgaagttgtt gatctgtccg tggtgatggt tgatgatcgc
     1441 gcccacccgg ctgaaatatc ggttcagcac gtggacgacg tcgtaaggca agtgcgcctc
     1501 ggcgaacgcg gtgaagttgc ggacgtcggc gaacaggatg gcgaggtgac gctcctcgcc
     1561 cacgctgcgc ggcgtcgcgc cgcccgccat ctcctgatcc accaactgca cgtcctcgtc
     1621 gtccagcacc aggcggcgca gggccacgtc gccgctcaca gtcgtctggc aggccaggcg
     1681 caccatcggg ctgaaattgc ggcgcgtcgc catcttctgc tctttttcgt tgcgcgggca
     1741 gcaatagtcc aggccctcca gaatcaacac ccggcacgtc gagcagcgcg cattcccgcc
     1801 gcagacgtgc gtgtgcggga tgccatgctg gagcgaggcc tgcaggatgg gcgtggtcgt
     1861 gtctacttca accacagctt cgttttgata cgtgatatgc ggcatacgga ctccccgaag
     1921 cgggatcact tcctcgcgac gcgaattata cgccgttccg gcgcttcgcg ctttg
//
LOCUS       DBNGWZ010000423         6312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3452339, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000423 DBNGWZ010000000
VERSION     DBNGWZ010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3452339"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /gene="otsB"
                     /locus_tag="AC16CX_12770"
     CDS             <1..808
                     /gene="otsB"
                     /locus_tag="AC16CX_12770"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3013420.1"
                     /translation="SGEGMQVLSPRVDLAAFYDRLAAAHSRVLMLDYDGTLAPFRIRP
                     EQAIPYPGVAEALRALIDEGDIRVVIVSGRRASEVAALLPPGKQPEIWGAHGWERIAP
                     SGEIRVQEPDAAVCAALAQAETHAAELICEGARLERKPASIAVHWRGLPVLTVARLRE
                     QLSSAWKAVAEEGVLEPLPFDGGLELRARGTNKQHAVKVVLSETAPESPIAYLGDDVT
                     DEDAFRAVKAQGLAVLVRSEYRETAADVWIRPPHELLAFLDRWRRKEDAR"
     gene            805..1872
                     /locus_tag="AC16CX_12775"
     CDS             805..1872
                     /locus_tag="AC16CX_12775"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3013421.1"
                     /translation="MKLLFQAWHANQPFDLAVAAGIALGVMLAFAALRFGATRALKAL
                     AARTSAVVFEIAVDVAGATKLWLLLPVALYAGASAIELPGRIERLIDVAALVALLLQA
                     AILANRFILSWLQRKVARQRVVDGGVVTALELIGFAARAVVWGLTGLFILGHLGFDVT
                     ALVAGLGIGGVAVALAVQNILGDLFASLSIVLDKPFVVGDFIVVDHLRGTVERVGVKT
                     TRLRSLDGELLVFSNADLLKSRIRNFKRMFDRRVEFTVGVVYQTPLEKLRLVPQWLRE
                     CVEAQQRVRFDRAHFKQYGDSALVFEVVYYVLDPNYNAYMDTHQAVNLAIFERFAREG
                     VGFAYPTRTLHLDTALAAAAG"
     gene            1876..3399
                     /locus_tag="AC16CX_12780"
     CDS             1876..3399
                     /locus_tag="AC16CX_12780"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR3013422.1"
                     /translation="MNLLHEQAMMRRRDRLIVVSNRLPYAFRRDDAGRWKAEPSGGGL
                     VTALLPVLRHRGGTWIGWPGSTDEAPGLDVALAGVGEDTGYTLQAVRLTAEEVRNFYG
                     GFSNEVIWPLFHDMQLLCNFDPLYWRTYREVNRKYAEVVHDSSGPGDFIWVHDYHLMN
                     VAAELRRLGSQSRIGFFLHIPFPSPDIYFKLPWRNALIETLLQYDLIGFQTLRDRRNF
                     VQCVRTLLRGVEFEGRGQVLQATVSGRQVRVGNFPISIDFNAFMRQAATAEVAARARE
                     LHRLLPNRKLVLGIDRLDYTKGIPLRLRAFHDVLTRYPDLRERVSLIQVVVPSREEIG
                     QYRDLKTEIEQLAGKINGSFVRPGGWVPVWYEYRTLSRTDLLAYYRAADIALVTPLKD
                     GMNLVAKEYCACSIEEDCALILSEFAGAAAQLARGALLVNPYDIEGVAETIRRAYHMD
                     KAERRARMRRLRRSIRDNDVFWWVDSFLRAAITKDLRAFPLPQNYVANESADYQLLF"
     gene            3548..4741
                     /locus_tag="AC16CX_12785"
     CDS             3548..4741
                     /locus_tag="AC16CX_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3013423.1"
                     /translation="MPSFLPQYPFPASPLLLFGLLLLAGYAGGEVVRRMLGLPRIVGY
                     VLIGMALGASGLNVLDAKLMREAWIFVDIALGLILFELGRRLDFAWLGRDPWLAATGV
                     LESALAFACVFLALLYFEVKPLHAAAAASIAIATSPAVVLVVAQELKAEGQVTERMLI
                     LTAMNSIIAFIASTMLLAWLHHEYRASWMTVVLHPAYLLAGSALLGYAASIAAMALAR
                     WLGKSERGHFVMLLALIVATVGAARVFELSVVLALLAFGVLSRNLDERHDLMPVDVGI
                     IGQLFVVVLFVVSGASLKIGEFVTGGTLALVYVLARFAGKSLGVMSLTYLSGVRRGAA
                     GLLCLALVPMSGTALGMVHRTSLLYPEFGAKLSAIVLSAVLILELIGPVAVQFALKRA
                     GEAAQ"
     gene            4753..5874
                     /locus_tag="AC16CX_12790"
     CDS             4753..5874
                     /locus_tag="AC16CX_12790"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HIR3013424.1"
                     /translation="MEFKQSEACSVGVELELQILNSRDYDLARDAADLIGLLEKTGHP
                     GAVKPEITESMIELNTSIHKGHDTLVAELKAIRDAVTHAAQRLNLRIAGGGSHAFHQW
                     TDRRIYPTERFTHLLELYGYLAKQFTIFGQHVHIGCPHGDAAIYLTHILSRFVPHFIA
                     LSASSPFQQGEDTSYASSRLNTVSAFPLSGQIPFVHSWSEFLAYFGKMHGYGIVESMK
                     DFYWDIRPKPEYGTVEIRVFDTPLTVERAAGLAAYAQTLARHILNDRPLAPERDVYTL
                     YNHNRFQACRYGLEGKIVDAYTLRHVSLKDDIMEMLMVIAPHAADLGNGEALARLAAD
                     VEAGRSDAAWLRQTHHERGSLNDVARVQSDLWMGLVQPA"
     gene            5967..>6312
                     /locus_tag="AC16CX_12795"
     CDS             5967..>6312
                     /locus_tag="AC16CX_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease/crotonase-like domain-containing
                     protein"
                     /protein_id="HIR3013425.1"
                     /translation="MRLLRMKLRRGFPAALVVIIASGLAGSLPAGESAAPAGVVQLAI
                     EGAVSPATADYAVRGLRRAADRAAQLVVLRLDTPGGLDTSMRQVIKEILASPVPVAAY
                     VAPSGARAASAGT"
BASE COUNT          960 a         2053 c         2173 g         1126 t
ORIGIN      
        1 ctcgggtgag ggcatgcagg tactgagtcc acgcgtcgac ctggcggcct tctacgaccg
       61 gctcgccgcg gcccacagcc gcgtgctgat gctcgactac gacggaacgc tcgcgccgtt
      121 caggatccgg ccggaacagg cgatcccgta ccccggtgtc gccgaagcgc tgcgcgcgct
      181 catcgacgag ggtgatatcc gcgtcgtgat cgtgagcggc aggcgcgcct ccgaggtcgc
      241 ggcattgctg ccgcccggca aacagcccga gatctggggc gcccatggtt gggagcgcat
      301 tgctccatcg ggagagatac gcgtccagga gccggacgcg gcggtgtgcg cggcgcttgc
      361 acaagcggaa acgcacgcgg ccgaattgat atgcgaaggg gcgcggctcg agcgcaagcc
      421 cgcgagcatc gcggtgcact ggaggggact gccggtactg accgtggcgc ggctgcggga
      481 gcaattgagc tcggcttgga aggcggtcgc tgaagagggc gttctggagc cgctgccgtt
      541 cgacggcggg ctcgagctgc gcgcgcgcgg caccaacaag cagcatgcag tgaaagtcgt
      601 gctctcggaa actgcgccgg aaagtccgat agcctatctg ggggacgacg tcaccgacga
      661 ggacgcattc cgcgcggtga aggcgcaggg gcttgccgtg ctggtccgct ccgaataccg
      721 cgagaccgcg gccgacgtgt ggatccggcc tccgcatgaa ctcctggcgt ttctcgaccg
      781 ctggcggcgg aaggaagacg cgcgatgaaa ctcctgttcc aggcctggca cgccaaccag
      841 ccgttcgatc tggcggtcgc cgccggcatc gcgctcggcg tcatgctggc gttcgcggcc
      901 ttgcggttcg gcgcgacgcg ggcgctcaag gcgctggcgg cgcggacctc ggccgtggtt
      961 ttcgagatcg cggtggacgt agcaggggcg accaagctgt ggctgctgct gccggtcgct
     1021 ctctatgccg gggcttccgc aatcgagctt cccggcagga tcgagcgtct gatcgacgtg
     1081 gcggcgctgg tggccctgct tctgcaggcc gccatattgg ccaaccgctt catccttagt
     1141 tggctgcaac gcaaggtggc gcggcagcgg gtcgtcgacg gcggcgtggt cactgcgctc
     1201 gagctgatcg gttttgcggc gcgcgcggtg gtgtgggggc tgacggggct tttcatactc
     1261 ggccacctcg gcttcgatgt caccgcgctg gtggcggggc tcggaatagg gggggtcgcg
     1321 gttgcgctgg cggtgcagaa catcctcggc gacctgttcg cgtcgctttc catcgtgctc
     1381 gacaagccgt tcgtggtcgg cgatttcatc gtggtggacc acctgcgcgg cacggtcgag
     1441 cgcgtcggcg tcaagaccac ccgcctgcgc agcctcgacg gcgagctgct ggtgttctcg
     1501 aacgccgatc tgctgaaaag ccgcatccgc aacttcaagc gcatgttcga ccgccgcgtc
     1561 gaattcaccg tgggcgtggt ttatcagacg ccgctggaga agcttcggct cgtcccgcaa
     1621 tggctgcgcg agtgcgtgga agctcagcaa cgggtgcgct tcgaccgggc gcacttcaag
     1681 caatacgggg attcggccct cgttttcgag gtcgtgtatt acgtgctcga ccccaattac
     1741 aacgcctaca tggacacgca ccaggcggtc aacctcgcca tcttcgagcg cttcgcgcgc
     1801 gagggcgtcg gattcgccta tcccacgcgc acactccatc tggatacggc gcttgccgcg
     1861 gcggcggggt gacgcatgaa cctcttgcat gagcaggcga tgatgcgccg ccgcgaccgg
     1921 ttgatcgtgg tgtcgaatcg gcttccgtac gcgttccgcc gcgacgatgc cgggcgctgg
     1981 aaagcggagc cgagcggcgg cggcctggtg accgccttgc tgccggtgct gcggcaccgc
     2041 ggcggcacct ggatcggctg gcccggcagc accgatgagg cgccgggcct cgacgtcgcg
     2101 ctggccgggg tcggtgagga cacgggctac acgttgcaag ccgtgcggct cacggcggag
     2161 gaggtgcgca acttctacgg cgggttctcg aacgaggtca tctggccgct gttccacgac
     2221 atgcagctgc tgtgcaactt cgatcctctc tactggcgca cctaccgcga ggtgaaccgg
     2281 aagtacgcgg aggtggtgca cgattcgagc ggtcccggcg acttcatctg ggtgcacgac
     2341 taccatctca tgaacgtcgc cgccgagctg cgccggctcg gttcacagtc ccgcatcggc
     2401 ttcttcctgc acatcccttt tccgtcgccc gacatctatt tcaagcttcc ctggcgcaac
     2461 gccctgatcg agacgctgct gcagtacgat ctcatcgggt tccagaccct gcgcgaccgg
     2521 cggaacttcg tgcagtgcgt gcgcacgctg ctgaggggtg tcgagttcga aggacgcggc
     2581 caggtgctgc aggcgacggt ttccgggcgc caggtgcgcg tcggaaactt ccccatcagc
     2641 atcgacttca acgctttcat gcgccaggcc gccacggcgg aagtcgcggc gcgcgccagg
     2701 gaactgcacc gcctgctgcc caaccgcaag ctcgtcctgg gcatcgaccg ccttgactac
     2761 accaagggca tcccgctgcg gctgcgggcc ttccacgacg tgctgacgcg ctatcccgac
     2821 ctgcgcgagc gcgtctcgct catccaggtg gtggtgccga gccgcgagga aatcggccag
     2881 taccgcgacc tcaagaccga gatcgagcag ctggctggca agatcaacgg cagcttcgtg
     2941 cggccggggg gctgggtgcc ggtgtggtac gagtaccgca ccctcagccg caccgatctg
     3001 ctggcctact accgcgccgc ggacatcgcc ctggtgacgc cgctcaagga cggcatgaac
     3061 ctggtggcga aggagtactg cgcctgcagc atcgaggagg attgcgcgct gatcctgtcc
     3121 gagttcgcgg gagccgcggc gcagctcgcg cgcggcgcgc tgctcgtgaa tccgtacgac
     3181 atcgagggtg tcgccgaaac gatccgccgg gcttatcata tggacaaggc cgaacgccgt
     3241 gcgcggatgc gccgcctgcg ccgctcgatc cgcgacaacg acgtattctg gtgggtggac
     3301 tcgtttctgc gcgcggcgat caccaaggac ctgcgcgcgt ttccgctgcc gcagaactac
     3361 gtcgccaacg aaagcgcgga ctatcagctg ctgttctgag cgcgcggcgc cgggaacttt
     3421 tccggcgctc ggggttttgt gaacgaaagg cgagagggag cgccggcccg cgaccggtcc
     3481 gcgccacatt cagcaagccc ctctccatgc tggaggcata acagatccga taaggggacc
     3541 gccagtcatg ccgagcttcc tgccccagta tccattcccg gccagcccgc tgttgctgtt
     3601 cggcctgctg ctgctcgcgg gatacgcggg cggcgaggtg gtgcggcgca tgctgggact
     3661 gccgcgcatc gtcggttacg tgctgatcgg gatggcgctc ggcgcgagcg ggctcaacgt
     3721 gctggacgcg aagctgatgc gggaggcctg gatcttcgtc gatatcgcgc tcgggctgat
     3781 cctgttcgag ctcggcagac gcctcgactt cgcctggctc gggcgcgatc cctggctcgc
     3841 ggcgaccggg gtgctcgaga gcgctctggc gttcgcctgt gtcttcctcg cgctcctcta
     3901 cttcgaggtc aagccgctcc atgcggcggc agccgcctcg atcgcgatcg ccacctcgcc
     3961 cgccgtggtg ctggtcgtgg cgcaggagct gaaagcagag ggacaggtga cggagcggat
     4021 gctcatcctc accgcgatga acagcatcat cgccttcatc gcatccacga tgctgctcgc
     4081 gtggctgcac catgaatacc gcgccagctg gatgaccgtc gtgctgcatc cggcctatct
     4141 gctggccggc tcggcgttgc tgggctatgc ggcgagcatc gccgccatgg cgctggcgcg
     4201 ttggctcggg aagagcgagc gcgggcattt cgtgatgttg ctcgccctca tcgtggcgac
     4261 ggtcggcgcc gcgcgcgtgt tcgagctctc ggtcgtactc gcgctgctcg cattcggggt
     4321 gctgtcgagg aacctcgacg agcggcacga tctgatgccc gtggacgtgg gcatcatcgg
     4381 gcagctgttc gtggtcgtgc tgttcgtggt gagcggcgca agcctcaaga tcggtgagtt
     4441 cgtgaccggc ggcacgctgg cgctcgtcta cgtgctggcc cggttcgccg gcaagtcgct
     4501 cggcgtcatg agcctgacct acttgagcgg cgtgcgccgc ggcgccgcgg gcttgctgtg
     4561 cctggcgctc gtgcccatgt cgggaacggc gctcgggatg gtgcaccgga catcgcttct
     4621 gtacccggaa ttcggcgcga agctttccgc gatcgtgctt tcggcggtgc tgatcctgga
     4681 gttgatcggg ccggtcgcgg tgcagttcgc gctcaagcgc gcgggggagg cggcgcagtg
     4741 agcgatgccg gaatggaatt caagcagtcc gaggcctgct cggtgggcgt cgagctcgag
     4801 ctgcagatcc tcaactcgcg cgactacgat ctcgcgcgcg acgccgcgga cctgatcggg
     4861 ctgctggaga agaccggcca tcccggggcg gtgaagcccg agatcaccga gagcatgatc
     4921 gaattgaaca cctcgatcca caagggccac gacacgctgg ttgcggaact gaaggcgatc
     4981 cgcgacgcgg tgacgcacgc cgcgcagcgg ctcaacctgc gcatcgcggg cggcggctcc
     5041 cacgccttcc accagtggac cgaccgccgc atctatccca ccgagcgttt cacgcacctg
     5101 ctcgagctct atggctacct cgccaagcag ttcacgatct tcgggcagca cgtgcatatc
     5161 gggtgcccgc acggcgacgc ggcgatctac ctgacccata tcctctcgcg cttcgtgccg
     5221 cacttcatcg cgctttccgc ctcctcgccg ttccagcagg gcgaggacac ctcctatgca
     5281 tcgtcgcggc tcaatacggt cagcgcattc ccgctgtcgg ggcagatccc gttcgtgcat
     5341 tcctggagcg agtttctcgc ctatttcggg aagatgcacg gctacgggat cgtcgagagc
     5401 atgaaggatt tctactggga catccggccc aagcccgagt atggaacggt ggaaatccgc
     5461 gtattcgaca cgccgctcac cgtggagcgc gcggcagggc tcgcggccta cgcccagacg
     5521 ctcgcgcggc acatcctgaa cgaccggccg ctggcgcccg aacgcgatgt ctatacgctc
     5581 tacaatcaca accggttcca ggcctgccgc tacgggctcg aggggaagat cgtcgatgcc
     5641 tacaccctgc ggcacgtcag cctcaaggac gacatcatgg agatgctgat ggtcatcgcg
     5701 ccgcacgccg ccgatctcgg caacggcgag gcgctggcgc gcctcgcggc cgatgtcgag
     5761 gcgggccgct cggacgcggc atggctgcgc cagacccacc acgagcgcgg ctccctgaac
     5821 gacgttgcgc gcgtgcagag cgacctctgg atggggctcg tccaacctgc ctgaatgatc
     5881 ttttattttt gtcatggcgc tggttttgca ttaggaattt gccggatctc agtccggcaa
     5941 attcctgggg ggagtatgat ggttccatgc gattactgcg catgaaactg cggcgcggat
     6001 tcccggccgc gcttgtcgtc atcatcgctt caggactggc ggggagcctg ccggcgggcg
     6061 aatccgcggc gccggcgggg gtagtgcagc ttgcgatcga gggggcggtg agcccggcga
     6121 cagccgacta tgccgtgcgc ggcttgcgcc gggcggccga tcgcgccgcg cagctggtgg
     6181 tgctgcggct cgatacgccc ggcggcctcg atacctcgat gcgccaggtg atcaaggaaa
     6241 tcctggcttc gccggttccg gtcgcggcgt acgtggcgcc gagcggcgcg cgcgcggcga
     6301 gcgccgggac ct
//
LOCUS       DBNGWZ010000424         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_933470, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000424 DBNGWZ010000000
VERSION     DBNGWZ010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_933470"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="AC16CX_12800"
     CDS             <1..327
                     /locus_tag="AC16CX_12800"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3013426.1"
                     /translation="TTQRGGYRVFSTPSMTQFVEMTAQQLAAPHLKPGQGQVGTLVTI
                     RHMAPTPIGKKVRAEAELVGIDRRKLLFKVKVYDDVEQVGEAEHERFIIDEDKYIERL
                     KKKIEA"
     gene            complement(405..1286)
                     /locus_tag="AC16CX_12805"
     CDS             complement(405..1286)
                     /locus_tag="AC16CX_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013427.1"
                     /translation="MPFASSKGVQLYYEEAGRGFPAVFVHEFAGDLRSWEPQLRYFSR
                     RYRCIAFNARGYPPSDVPEKVSQYSQAIATDDIANVMRSLGIRKAHIIGCSMGGYATL
                     HFGLRYARMARSLTVIGAGYGSDPDKRAQFLRDTEVMARRFETLGTPEAISIYQVGPA
                     RVQLQNKDPRAFREFCEEFAEHPALGSANTLRGVQARRPTIYNLERGLRRMRVPTHII
                     TGDEDNQCLDPGIFIKRVCPAARLTVVPATGHAVNIEEPDLFNRITDDFLTVVDSGRW
                     RPRDPRSYNPSTMARKA"
     gene            complement(1445..2296)
                     /locus_tag="AC16CX_12810"
     CDS             complement(1445..2296)
                     /locus_tag="AC16CX_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3013428.1"
                     /translation="MAALLAGAAAIATAPLFVRVSETGPIATAFWRIALALPLLWAWS
                     AVEQRGNLAASFAAERRLIIAAGLFFAGDLAVWHWSIMLTSVANATLLANLAPIFVTL
                     AVWALFSRRPTAMFLAGLSTALAGVAVLIGGDFRLGEKALAGDALGVITAMFYAAYQL
                     TITRLRERVATSSIMAWSGIFMAVPLLVLALVTGEQIMPLTTSGWVKLAALAVIAQVA
                     GQSLIAYAMAHLPATFSSVGLLLQPVMAALYAWVLLGEALGWLEIAGGVAVLVGIRIA
                     QKAELGR"
     gene            complement(2363..>3121)
                     /locus_tag="AC16CX_12815"
     CDS             complement(2363..>3121)
                     /locus_tag="AC16CX_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3013429.1"
                     /translation="LLERPAESVVLLRINRPEARNALNLEVRKLLAKHLSELGEDTTT
                     RAIVLTGNEKSFAAGADIKEMADLGTIEALQRGVHKLWRAIAGCPKPVIAAVSGYALG
                     GGCELAMTCDIIVAGESARFGQPEVNIGIIPGGGGTQRLTRAMGKYKAMKYILTGEMF
                     TATEAFEMGLVSEVVPDPEVEGRAIDLARQIAVLPPLAVQLAKEAVLAGMDAPLTSAL
                     ALEAKSSQILFSSQDQKEGMRAFIEKRKPKFSGR"
BASE COUNT          554 a         1018 c         1043 g          506 t
ORIGIN      
        1 accacccagc gcggcggcta ccgggtgttt tccacgccgt cgatgacgca gttcgtggag
       61 atgacggcgc agcagcttgc cgcgcctcac ctgaagcccg gccaggggca agtcgggacc
      121 ctcgtcacga tccgccacat ggcgccgacg cccatcggga agaaagtgcg cgccgaagcc
      181 gagttggtcg gcatcgaccg gcgcaagctc ctgttcaagg tgaaggttta cgacgacgtc
      241 gagcaggtgg gcgaagccga gcacgagcgc ttcatcatag acgaggacaa gtacatcgag
      301 cggctcaaga agaaaataga ggcgtgataa gtgacgattg acaagggata aggcacccgc
      361 gctcgcgcgg aagctggctc ctatcaccta tcacttatca cccgtcacgc ttttctggcc
      421 atcgtagatg gattgtagga gcgcggatcg cgcggccgcc agcggcccga gtcaacgacc
      481 gtcaagaagt cgtccgtgat gcggttgaag agatcgggct cctcgatgtt gacggcatgc
      541 ccggtcgcgg gcaccaccgt gagccgcgcc gcggggcaca cgcgcttgat gaaaatcccc
      601 ggatcgagac actggttgtc ctcgtcgccg gtgatgatgt gggtcggcac cctcatgcgg
      661 cgcaagcccc gctccaggtt gtagatcgtc ggacgccgag cctgcacgcc gcgcagcgtg
      721 ttggcggagc cgagcgccgg gtgctcggcg aactcctcgc agaattcgcg gaaggcgcgc
      781 gggtccttgt tctgcaactg cacgcgcgcc ggtccgacct ggtagatcga gatggcctcc
      841 ggcgtgccca gcgtctcgaa gcggcgcgcc atcacctcgg tgtcgcgcag gaattgcgcg
      901 cgcttgtcgg ggtcggagcc gtaccccgcg ccgatcaccg tgagcgagcg cgccatgcgc
      961 gcatagcgca atccgaagtg cagcgtggca tagcccccca tcgagcagcc gatgatgtgc
     1021 gccttgcgga tgccgaggct ccgcatgacg ttggcgatgt cgtcggtcgc gatcgcctgc
     1081 gagtactgcg acaccttttc cggcacgtcg gaaggcggat agccccgcgc gttgaacgcg
     1141 atgcagcggt agcggcggct gaaatatcgc agctgcggct cccagctgcg caggtcgccc
     1201 gcgaactcgt ggacgaacac ggccgggaat ccgcgtcccg cctcctcata gtagagctgg
     1261 acgcctttcg aactggcaaa tggcattttg ccccctaaaa aagtgattcg tggatcgtgg
     1321 atcgagtctt actgatgacc tggttgtaga ggatcgtgaa gttacgaccc acgacccacg
     1381 cctttctata ggcgccttcc aagccggata ggataattaa acacgctact gtgcggcaag
     1441 cgcgttatcg cccgagctcg gctttttggg cgatgcggat gccgacgagg acggcgacgc
     1501 cgccggcgat ctcgagccac cccagcgcct cgccgagcag gacccaggcg tagagcgcgg
     1561 ccatgaccgg ctgcagcagc aacccgaccg aggagaacgt ggcgggcagg tgcgccatgg
     1621 cgtaggcgat caggctctgg cccgcgacct gcgcgatgac ggccagcgcg gcgagcttca
     1681 cccagcccga cgtcgtgagc ggcatgatct gctcgcccgt gacgagcgcg agcacgagca
     1741 gcggcaccgc catgaagatg ccgctccagg ccatgatgct ggaagtcgcc acacgctcgc
     1801 gcaacctcgt gatggtgagc tggtaggcgg cgtagaacat cgcggtgatc accccgaggg
     1861 catcgcccgc gagcgccttt tcgccgagac ggaaatctcc gccgatcaac accgcgacgc
     1921 ccgcaagcgc ggtcgagagc cccgcgagga acatcgcggt cggccgcctg ctgaacagcg
     1981 cccataccgc gagcgtgacg aagatcggcg cgaggttggc gagaagcgtg gcattggcaa
     2041 ccgaggtgag catgatggac cagtgccaca cggcgaggtc cccggcgaag aacaggccgg
     2101 ccgcgatgat gagacgccgc tcggcggcga agctcgcggc aaggttgccg cgctgctcga
     2161 ccgcagacca tgcccagagc aagggcaggg cgagcgcaat gcgccagaac gcggtcgcga
     2221 tcggcccggt ttcgctgacc ctgacgaaca gcggcgcggt ggcaatcgcc gccgcgcccg
     2281 cgagcagcgc cgccacggcg attgcgcgcc tggagtcctg cgaatttaga tctggcggat
     2341 tcaatcaaat tttccggccg tgtcagcggc cggaaaattt gggcttgcgc ttctcgatga
     2401 acgcgcgcat gccctctttc tgatcctggg aggagaacag gatctgcgac gacttcgctt
     2461 cgagcgcgag cgcgctcgtg agcggcgcgt ccatcccggc gagcaccgcc tccttggcga
     2521 gctgcacggc aagcggcggc agcacggcga tctggcgcgc gaggtcgatc gcgcggcctt
     2581 ccacttcagg atcgggcacc acttcgctca ccagccccat ttcgaaggcc tctgtcgccg
     2641 tgaacatctc gccggtcaat atgtacttca tggccttgta cttgcccatg gcgcgcgtca
     2701 gccgctgcgt gccgccgccg ccgggaatga tgccgatgtt gacctcgggc tgcccgaagc
     2761 gggccgattc cccggcgacg atgatgtcgc aggtcatggc gagctcgcag ccgccgccga
     2821 gcgcgtagcc gctgaccgcc gcaatgaccg gcttgggaca gcccgcgatc gcccgccaca
     2881 gcttgtgcac cccgcgctgg agcgcctcga tggttccgag gtcggccatc tccttgatgt
     2941 cggcgccggc ggcgaagctc ttctcgttgc cggtgagcac gatggcgcgc gtcgtcgtgt
     3001 cttcccccag ctcggagagg tgcttcgcca gcagcttccg cacctcgagg ttgagcgcgt
     3061 tgcgtgcctc ggggcggttg atgcgcagca gcaccacgct ttctgccggg cgttcgagga
     3121 g
//
LOCUS       DBNGWZ010000425         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_427849, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000425 DBNGWZ010000000
VERSION     DBNGWZ010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_427849"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="AC16CX_12820"
     CDS             <1..1206
                     /locus_tag="AC16CX_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3013430.1"
                     /translation="RLDTPGGLDTSMRQVIKEILASPVPVAAYVAPSGARAASAGTFI
                     LYASHIAAMAPATNLGAATPVPIGIAPGGDQPKERGKREGKDAKKSGKSEAESGQEPD
                     SKSSMARKQMNDAAAYIRSLAQLRGRNAEWAEQAVREAVSLSAEEARKRKVIDLIATD
                     VTDLLKQLHGKKLTVQGVQRTLETQGAKVTAVEPDWRSRLLAVITDPSIALILMMIGI
                     YGLIFEFSNPGYVLPGVAGGICLLLALYAFQLLPVNYAGLALILLGLAFIVAEAFLPS
                     FGSLGIGGVVALVIGSVILFDPDVAAGYTLPLPFVVTLAATSGTVVFFTVWFALKARR
                     RPVVSGREEIVDASGVALADFEGEGWAQVRGETWRVVSTTPLARGQKLRVTGIEGLTL
                     RVEPEDGNR"
     gene            1276..>1997
                     /locus_tag="AC16CX_12825"
     CDS             1276..>1997
                     /locus_tag="AC16CX_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR3013431.1"
                     /translation="MIEQLLGTGGVIIFIVILVVASLRILREYERGVVFLLGRFWKVK
                     GPGLIVIVPGIQQMVRIDLRIVVLDVPAQDVVTRDNVSVKVSAVVYFRVIDPQKAVIQ
                     VANFLEATNQFAQTTLRAVLGKHELDELLAERDKLNLDIQKILDAQTDGWGIKVSNVE
                     IKHVDIDVSMVRAIAQQAEAERTRRAKVIHAEGELQAAEKLLQAARMLAQQPQAMQLR
                     YLQTLGSIAGDKSNTIVFPVPM"
BASE COUNT          343 a          624 c          700 g          330 t
ORIGIN      
        1 cggctcgata cgcccggcgg cctcgatacc tcgatgcgcc aggtgatcaa ggaaatcctg
       61 gcttcgccgg ttccggtcgc ggcgtacgtg gcgccgagcg gcgcgcgcgc ggcgagcgcc
      121 gggaccttca tcctgtacgc cagccacatc gcggcgatgg cgcccgccac caatctcggc
      181 gccgccaccc cggtgccgat cggcatcgcg cccggcgggg accagcccaa ggagcgcgga
      241 aagagggaag ggaaggatgc gaagaagtct ggcaagtccg aggcggagtc ggggcaggag
      301 cccgattcca agagcagcat ggcgcgcaag cagatgaacg atgccgccgc gtacatccgc
      361 agcctcgcgc agctgcgggg acgcaacgcc gagtgggcag agcaggcggt gcgcgaggcg
      421 gtgagcctgt cggcggaaga agcccgcaaa cgcaaggtca ttgacctgat cgcgaccgac
      481 gtgacggacc tcctcaagca gctccacgga aagaagctca ccgtccaggg cgtgcagcgc
      541 acgctcgaga cgcagggcgc caaggtcaca gcggtggagc cggactggcg cagccggctc
      601 ctcgcggtca tcaccgatcc gagcatcgcg ctcatcctga tgatgatcgg gatctacggc
      661 ctgatcttcg agttttccaa tcccggctac gtgcttccgg gggtcgcggg cgggatctgc
      721 ctgctgctgg cgctctacgc cttccagctg ctgccggtga actacgcggg gctggcgctg
      781 atcctgctcg ggctggcgtt catcgtcgcc gaggcgttcc tgcccagctt cggctcactc
      841 ggtatcgggg gtgtggtggc gctggtgatc ggctcggtga tcctgttcga tcccgatgtc
      901 gccgccggct acactctgcc gctgcccttc gtggtgacgc tcgcggcgac aagcgggacc
      961 gtcgtgttct tcaccgtgtg gttcgcgctc aaggcgcggc gccggccggt ggtgagcggt
     1021 cgcgaggaaa tcgtggacgc gagtggcgtc gcgcttgccg atttcgaggg cgagggctgg
     1081 gcgcaggtgc ggggcgagac gtggcgcgtg gtgagcacga caccgctcgc ccgcggccag
     1141 aagctgcggg tgaccggcat cgaaggcctc accctgcggg tcgagcctga agacggtaac
     1201 cggtagctcg gttccttcca gtcactagtc accagtcacg agtcacgagt cactgttctt
     1261 gcagggaggt gcgtcatgat cgaacagttg ctgggtacgg gcggcgtcat catcttcatc
     1321 gtaatcctcg tcgtcgcgtc gctgcgcatc ctgcgcgagt acgagcgcgg cgtcgtgttc
     1381 ctgctcggcc gcttctggaa ggtcaagggg ccggggctga tcgtcatcgt ccccggtatc
     1441 cagcagatgg tgcgtatcga tctgcgcatc gtggtactgg atgtgccggc gcaggacgtg
     1501 gtgacgcgcg acaacgtatc ggtgaaggtc agcgcggtag tgtatttccg cgtgatcgac
     1561 ccgcagaaag cggtgatcca ggtggcgaat tttcttgaag ccacgaacca gttcgcgcag
     1621 accacgttgc gcgcggtgct cggcaagcac gagctcgacg agctgctcgc cgagcgcgac
     1681 aagctcaatc tcgacatcca gaagatcctc gacgcgcaga ccgatggctg gggcatcaag
     1741 gtgtccaacg tggagatcaa gcacgtcgac attgatgtct cgatggtgcg ggcgatcgcg
     1801 cagcaggccg aagccgagcg cacacggcgc gccaaggtga tccacgccga gggcgagctg
     1861 caggccgcgg aaaagctcct gcaggcggcg cgcatgctcg cccagcagcc gcaggcgatg
     1921 cagctgagat atttgcagac cctcggcagc atcgccggcg acaagagcaa caccatcgtg
     1981 ttcccggtgc cgatgga
//
LOCUS       DBNGWZ010000426         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1313095, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000426 DBNGWZ010000000
VERSION     DBNGWZ010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1313095"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="AC16CX_12830"
     CDS             <1..1311
                     /locus_tag="AC16CX_12830"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3013432.1"
                     /translation="CPVCVPARAAFAVGKYVHQIGFWDNADAYDGSIPSWHHRLRESG
                     HRVVSIGKLHFRGHDGDDNGFSEEIIPMNIIEGKGDLMGLVRKDLPERGLSWKIAKLA
                     GPGETPYTAYDREITTRAQIWLREEAPGYRDKPWVLFVSFVCPHFPLVAPADFYYQYP
                     LDKMPLPKQYAKEERPDHPYLRDYAGSFVYDRYFDPGKLRKAVAAYYGLCSFLDDNIG
                     RVLRTLEAVGLAGSTRVLYTSDHGDNLGARGLWGKSTMYEEVAGVPLIIAGTGVPQGK
                     KVSTPASHVDTYPFILECAGEARPAMYDGHPGVSLTKLARGERPDRNVLSEYHGMGST
                     TGAFMIRHGKYKYVHYVAYRPQLFDLEADPEELHDLAGEPGHARVLEECRARLYAICD
                     PAEVDARAKQRQAELLQEAGGREAVIRRGDLGFTPPPGYPAVFG"
     gene            1350..2324
                     /locus_tag="AC16CX_12835"
     CDS             1350..2324
                     /locus_tag="AC16CX_12835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013433.1"
                     /translation="MIRNSVMFFMLSAVAGVTQTALAQEFPARPIRMLVAFAPGGNTD
                     ILARAVGIRMTESWGKPIVVDNRPGGSGVLAAEIVAKASPDGHTIFVASTGELSINPS
                     LFKKLPYDVQRDYAPVSLGTVSPLLIVVHPSFAVTTLKGLLDAARAKPGALSYASVGV
                     GTPQHLSGELFKMATGTDIVHVPYKGGAPATTALLGGREVQFGFVGMGPVIPHVKAGR
                     LRPLAISTAKRSTHLPEVPTLQEQGVKDFDTSIWFAFVVPAKTPKGVIGKLNAEIGRI
                     LRKTEARDYLLGTGVEIAPTTPDELARFIKSEAEKYRRIIEVSGTKAD"
     gene            2350..>2978
                     /locus_tag="AC16CX_12840"
     CDS             2350..>2978
                     /locus_tag="AC16CX_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3013434.1"
                     /translation="MRKTCAIVSCAIGLAAPVAALAQAYPAKPIRVVVPYAPGGATDL
                     TARLVGQKMQEAMKQNVLVENRPGAGGVIGADNVARAAPDGYSVLLAVPAEIAILPHL
                     QKMPYNVEKDLAPVSLAAVTPLVLVVHPSLPVKTVKELVEFIRKRPGQLSYASAGTGG
                     VQHLSGELLKITMKLDMVHVPYKGAGPVMPDLIGGHVPMFFSGMPPAMP"
BASE COUNT          541 a          939 c         1018 g          480 t
ORIGIN      
        1 tgcccggtgt gcgtgccggc gcgcgccgcc ttcgcggtcg gcaagtacgt gcaccagatc
       61 ggcttctggg acaatgccga cgcctacgac ggctcgatcc cgagctggca ccatcgcctg
      121 cgcgaaagcg gccaccgcgt agtgtcgatc ggcaagctcc atttccgcgg gcacgacggg
      181 gacgacaacg gcttcagcga agagatcatc cccatgaaca tcatcgaggg caaaggcgac
      241 ctcatggggc tcgtgcgcaa ggacctgccc gagcgcggcc tgtcgtggaa aatcgcaaag
      301 ctcgccgggc cgggcgagac gccgtatacc gcgtacgacc gcgagatcac gacacgcgcg
      361 cagatctggc tgcgagaaga ggcgcccggg taccgcgaca agccgtgggt gctgttcgtc
      421 tccttcgtgt gcccgcattt tccgctggtg gcgcctgcgg atttctacta tcagtacccc
      481 ctcgacaaaa tgccgctgcc gaagcagtac gcgaaggagg agcggcccga ccatccgtat
      541 ctgcgggatt acgctggcag cttcgtttat gaccgctact tcgatccggg aaagctcagg
      601 aaggcggtgg ctgcatacta cgggctgtgc agtttcctcg atgacaacat cggtcgggtc
      661 ctgcgcacgc tggaggcggt cggcctcgcc ggttccacgc gcgtgctgta cacgagcgac
      721 catggcgaca acctcggcgc gcgcggattg tggggtaaat ccacgatgta cgaggaagtg
      781 gcgggcgtgc cgctcatcat cgccgggacc ggcgtgccgc aaggaaagaa ggtgagcacc
      841 ccggcaagcc acgtggacac ctaccccttc atcctggaat gcgcgggtga ggcccgtccc
      901 gcgatgtacg acgggcatcc cggcgtgtcg ctcacgaagc tcgcgcgcgg cgagcggccc
      961 gatcgcaacg tcctgtccga gtatcatggc atgggctcga cgaccggcgc gttcatgatc
     1021 cggcacggga agtacaagta cgtccactac gtcgcctacc ggccgcagct gttcgatctg
     1081 gaggccgacc ccgaggagtt gcacgacctc gccggcgaac cgggccatgc tcgcgtgctg
     1141 gaggagtgcc gcgcgcggct ctacgcgatc tgcgacccgg cggaagtgga tgcgcgcgcg
     1201 aaacagcgcc aggcagagct gctgcaggag gccggcggcc gcgaagcggt catcaggcgc
     1261 ggagacttgg gctttacgcc gccgccgggc tatccggccg tgttcggctg agggatgagg
     1321 gaagaagacc tgaaagcgcg gaggaaaaga tgattaggaa ttccgtgatg ttcttcatgc
     1381 tgtctgcggt cgcaggggtg acgcagacag cattggcgca ggaatttcca gccagaccga
     1441 tcaggatgct ggtcgcgttc gcgcccggcg ggaacaccga tatcctcgcg cgcgcggtgg
     1501 gcatacgcat gacggaaagc tggggcaagc cgatcgtggt ggataaccgt cccggtggca
     1561 gcggcgtgct ggccgcggaa atcgtggcga aggcgtctcc cgacggccac accattttcg
     1621 tcgcgtcgac cggcgagctg tccatcaacc ccagcctgtt caagaagctg ccatacgacg
     1681 tgcagcgcga ttacgccccg gtctcgctcg gcaccgtctc gccgctgctg atcgtcgtcc
     1741 atccatcgtt cgcagtgacg actttgaagg gactgctgga tgccgcccgt gccaagccag
     1801 gcgcactgtc ctacgcctcc gtcggcgtcg gcacgccgca gcatctgtcc ggcgagcttt
     1861 tcaagatggc gacggggacc gacatcgtgc acgtgccgta caagggcggc gcaccggcga
     1921 ccacggccct gcttggcggc cgggaagtgc agttcggctt tgtcggcatg gggcccgtca
     1981 ttccgcacgt gaaggccggc aggctgaggc cgcttgcgat ctccaccgcg aagcgttcga
     2041 cccatctgcc ggaggtgccc acgctccagg agcagggcgt gaaggatttc gacaccagca
     2101 tctggtttgc cttcgtcgtt ccggcgaaaa cgccgaaagg cgtcatcggc aagctcaacg
     2161 ccgagatcgg gcgcatcctg aggaagacgg aagcgcgcga ttacctgctc ggcaccggcg
     2221 tcgagatcgc gcccaccacg ccggatgagc tggcgcgctt cataaaatcg gaagcggaga
     2281 agtatcgcag gatcatcgaa gtgtccggca cgaaagcgga ctgaccgctg ggacaggagg
     2341 aggtggcaga tgaggaagac ctgtgcgatc gtaagctgcg cgatagggct ggcggcgcca
     2401 gtcgccgctc ttgcgcaagc ctatccggcc aagccgatcc gcgtggtggt gccgtacgcg
     2461 ccggggggcg cgaccgatct caccgcgcgc ctggtcggcc agaagatgca ggaagcgatg
     2521 aagcagaacg tgctggtgga aaaccggccc ggcgcgggcg gcgtgatcgg ggcggacaac
     2581 gtggcgaggg cggcgccgga cggctacagc gtgctgctcg ccgtgcccgc ggaaatcgcg
     2641 atactgccgc acttgcagaa aatgccgtac aacgtggaga aagacctcgc gccggtgtcg
     2701 ctcgcggcgg tgacgcccct cgttctcgtg gtgcatcctt cgctgcccgt gaaaaccgtg
     2761 aaggagctgg tggagttcat ccgcaagcgc cccggccagt tgagctacgc ttctgcgggc
     2821 accggcggcg tgcagcatct ctccggcgaa ttgctcaaga tcacgatgaa gctcgacatg
     2881 gtgcacgtgc cgtacaaggg cgcgggaccg gtgatgccgg acctcatcgg cgggcacgtg
     2941 ccgatgttct tctcgggcat gccgccggcc atgcccca
//
LOCUS       DBNGWZ010000427         3834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1184026, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000427 DBNGWZ010000000
VERSION     DBNGWZ010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1184026"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(461..2530)
                     /gene="uvrB"
                     /locus_tag="AC16CX_12845"
     CDS             complement(461..2530)
                     /gene="uvrB"
                     /locus_tag="AC16CX_12845"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR3013435.1"
                     /translation="MTHPKPENPAARNAGPSLVSFPESPYRLHHLFTPAGDQPEAIEK
                     LVAGVRDGLAFQTLLGVTGSGKTYTMAHVIARLGCPALILAPNKTLAAQLYSEMREFF
                     PENAVEYFVSYYDYYQPEAYVPSKDLYIEKDSSINEHIEQMRLSATKSLLERRDAIIV
                     CTVSAIYGIGDPVDYHSMILHLREREKLTQRDAIQRLAVMQYERNELEFQRGVFRVRG
                     DVLDIFPAENSETAVRLTLFDDEIESIHLFDPLTGHVLQRVPRFTVYPSSHYVTPRAT
                     TLRAIEAIKEELRGRIDWFQKEGKLVEAQRIEQRTRFDLEMLAEMGFCKGIENYSRHL
                     SGRPPGEPPPTLIDYLPHDALMIIDESHVTIPQLGGMYRGDRSRKENLVDYGFRLPSA
                     LDNRPLRFDEFEKVIRRTIFVSATPAEYEKEHQAQVVEQVVRPTGLVDPAVEVRPAAT
                     QVDDLLSEISDRAKKSERILVTTLTKRMAEDLTDYLGEHGVKVRYLHSDIDTVERVEI
                     IRDLRLGQFDALVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARHI
                     NGAAILYADTVTGSMRRAIDETERRRRKQTAYNAAHGITPKGVSKRIKDIIEGVYDRE
                     EARGQLKAAQAEARYGAMDEKALAQEVKRLEKEMLEAARNLEFERAAQLRDQLKALKQ
                     RLFLEVV"
     gene            2711..>3834
                     /locus_tag="AC16CX_12850"
     CDS             2711..>3834
                     /locus_tag="AC16CX_12850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HIR3013436.1"
                     /translation="MSHSLLAGVEMAPRDPILGVTEAFNADTNPNKVNLGVGVYYDDN
                     GKVPVLECVRRAEQQLAESPLPRNYLPIDGLQAYDRAVQELLFGADSPALRDQRIVTV
                     QTLGGTGGLKVGADLLRRLNGSAQVWISDPSWENHQALFDYAGFKVQAYPYYDAATHG
                     VKFDAMIGALNKLPAGTVVVLHACCHNPTGVDLSADQWERVIEAVNARGIVPFLDIAY
                     QGFAEGFDADAAAVRRFAQACPVVFVSSSFSKSLSLYGERVGALSLVAETADEAARAL
                     SQLKRVIRTNYSNPPTHGGRAVVTVLTTPELRALWDRELSGMRERIKTMRRQLVEKIR
                     AIRSDFDFGFVVRQRGLFSYSGLSRGQVKRLREEYSLYAI"
BASE COUNT          625 a         1247 c         1261 g          701 t
ORIGIN      
        1 atcggcggtc agggtgatgg tgcaccatgc cgctagattc ctcatcgacg ttggtggtgt
       61 gggtggaaga tctctctcag agtctgtccg caacgcggca gataaccgcc gaagtctctc
      121 caccccatct ctttccagtg tcccaaagaa gagcgattcc gaccatacaa gccgccgagc
      181 agcgcagacc agcccggggt ttcggtcgag cactgggcga gcctcggctg cggtcgccct
      241 tctgaaaaga tcacccgccc gaagcctgaa tggaaagatg gtctggcgcg agttgcgcag
      301 gccgccgggc tggtcgagca gcggagggac accacacgag ttcacgaggt agtggcggcg
      361 aaccggggat cccctttctt tggtgacttt ctttggggaa gcaaagaaag tcaccggcac
      421 ccgggccgcc cccggcaatg cgcgacgcag tcgcgctaga ttacacaacc tccaaaaaca
      481 ggcgctgttt cagcgccttc aactggtcgc gtagctgcgc cgcgcgctcg aactcgaggt
      541 tgcgcgccgc ctccagcatc tccttctcga gccgcttcac ctcctgcgcg agcgccttct
      601 cgtccatcgc cccgtagcgc gcctcggcct gcgccgcctt cagctggccg cgcgcctcct
      661 cgcggtcgta caccccctcg atgatgtcct tgatgcgctt cgagacgccc ttcggcgtga
      721 tgccgtgcgc ggcgttgtag gcggtctgct tcctgcggcg gcgctcggtc tcgtcgatcg
      781 cccggcgcat cgagccggtg acggtatcgg cgtacaggat cgcggcgccg ttgatgtgcc
      841 gcgccgcccg cccgatagtc tggatcagcg accgctctga gcgcaggaag ccttccttgt
      901 cggcgtcgag gatcgccacc agcgacacct ccggaatgtc gagcccctcg cgcaacaggt
      961 tgatgccgac cagcgcgtcg aactgcccca gccgcaggtc gcggatgatc tcgacgcgct
     1021 ccacggtgtc gatgtcggaa tggagatagc gcaccttgac gccgtgctcg cccaggtagt
     1081 cggtgaggtc ctcggccatg cgcttggtga gcgtggtcac cagaatgcgt tcgctcttct
     1141 tcgcccggtc gctgatctcg gacagcagat cgtccacctg cgtcgcggcg ggccgtactt
     1201 ccaccgccgg gtcgaccagc ccggtggggc gcaccacctg ctccaccacc tgcgcctggt
     1261 gctccttctc gtactccgcc ggggtcgcgg aaacgaagat cgtgcgccgt atcaccttct
     1321 cgaactcgtc gaaccgcagc ggccggttgt cgagcgccga gggaaggcga aagccgtagt
     1381 ccacgaggtt ctccttgcgc gagcggtcgc cgcggtacat cccgcccaac tgagggatgg
     1441 tgacgtggct ctcgtctatg atcatcagcg catcgtgcgg caggtagtcg atcagcgtcg
     1501 ggggcggctc gcccggcggg cggcccgaga gatgccgcga atagttctcg atgcccttgc
     1561 agaagcccat ctcggcgagc atctcgaggt cgaagcgggt gcgttgctcg atgcgctggg
     1621 cttccaccag cttgccctcc ttctggaacc agtcgatgcg gccgcgcagc tcctccttga
     1681 tcgcctcgat cgcccgcagc gtggtcgcgc gcggggtgac gtagtggctg gagggataga
     1741 cggtgaagcg cgggacgcgc tgcagcacgt gcccggtcag cggatcgaac aggtggatgg
     1801 attcgatctc gtcgtcgaac agggtcagcc gcaccgcggt ttccgagttc tccgccggga
     1861 agatgtcgag cacgtcgccg cgcacgcgga acacgccgcg ctggaactcg agctcgttcc
     1921 gctcgtactg catcacggcg aggcgctgga tggcgtcgcg ctgcgtgagc ttctcccgct
     1981 cccgcaggtg caggatcatg ctgtggtaat ccacggggtc gccgatgccg tagatcgcgg
     2041 agacggtgca gacgatgatg gcgtcgcggc gctcgagcag cgacttggtg gcggagagcc
     2101 gcatctgctc gatgtgctcg ttgatgctgg agtccttctc gatgtagagg tccttcgacg
     2161 gcacgtaggc ctcgggctga tagtagtcgt agtacgatac gaagtactcg accgcgttct
     2221 ccgggaagaa ctcgcgcatc tcggaataga gctgcgccgc cagcgtcttg ttcggcgcca
     2281 ggatcagcgc cgggcagccg agccgcgcga tgacgtgcgc catggtgtag gtcttgccgg
     2341 agccggtgac gccgagcagc gtctggaacg cgaggccgtc gcgcacgccg gcgacgagct
     2401 tttcgatcgc ctcgggctgg tcccccgccg gggtgaacag atggtgcagc cggtacgggc
     2461 tctccgggaa gctgaccagg ctcgggcctg cgttcctggc cgccgggttc tcgggcttgg
     2521 gatgggtcat tacgaaaatg ggcgcgcagc tgaaccgata attgtcccac agcgacacgc
     2581 tccgccgcca gcatagttgc tggcggagcg cgaatatgtg cattccagtt acttcatggc
     2641 tcgccagcag gtagaatagg tttcgttgaa aatccctcgc cgctcttcca cgacttccca
     2701 cccgagaaaa atgagccatt cccttctcgc cggcgttgaa atggcgccgc gtgatccgat
     2761 tctgggcgtc accgaggcct tcaacgccga taccaatccc aacaaggtga acctgggcgt
     2821 cggcgtctac tacgacgaca acggcaaggt gcccgtcctg gagtgcgtcc ggcgcgccga
     2881 gcaacagctc gcggaaagtc cgctgccgcg gaactatctg ccgatcgacg gcctgcaggc
     2941 atacgaccgg gcggttcagg agttgctgtt cggcgcggac agcccggcct tgcgcgatca
     3001 gaggatagtg accgttcaga ctctgggcgg cactggcggt ctcaaggtcg gcgccgacct
     3061 gctgcggcgg ctgaacggca gcgcccaggt ctggatcagc gacccgagct gggaaaacca
     3121 ccaggcactc tttgattacg cgggattcaa ggtccaggcc tacccttatt acgacgcggc
     3181 cacgcatggc gtgaagttcg acgcaatgat cggcgccctg aacaagcttc ccgccgggac
     3241 cgtcgtggtg ttgcacgcct gctgccacaa cccgaccggg gtggacctgt cggccgatca
     3301 atgggaacgg gtcatcgagg ctgtcaacgc gcgcggcatc gttccgtttc tcgacatcgc
     3361 ctaccagggc ttcgccgaag gcttcgacgc cgatgccgcg gcggtgcgcc gcttcgcgca
     3421 ggcctgcccg gtcgtgttcg tgtcgagctc gttctcgaag tccctgtccc tttacgggga
     3481 acgggtgggc gcgctttccc tcgtcgccga aaccgccgac gaggcggccc gggcgctgag
     3541 ccagctgaag cgcgtgatcc ggaccaacta ctccaatccc ccgacccacg gcgggcgggc
     3601 ggtcgtgacg gtgttgacga caccggagct gcgcgcgctg tgggacaggg agctgagcgg
     3661 gatgcgcgag cgcatcaaga cgatgcggcg ccagctggtg gagaagatcc gcgccatccg
     3721 ctcggatttc gacttcggct tcgtcgtgcg gcagcgcgga ctgttttcct actcgggcct
     3781 ttcgcgcggg caggtgaaac gcctgcgcga ggaatattcc ctgtacgcca tcga
//
LOCUS       DBNGWZ010000428        13534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2823908, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000428 DBNGWZ010000000
VERSION     DBNGWZ010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2823908"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1671)
                     /locus_tag="AC16CX_12855"
     CDS             complement(232..1671)
                     /locus_tag="AC16CX_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013437.1"
                     /translation="MPNGTEPPPEPVGPPIKVTVTDVADSEVDYRKQGELQALGSKDS
                     GWIERLAAALWTALIRLLDPLIELAARAADKLLSAIAHFIDKAEAESSPEFYELLAAV
                     IEDLTGVHVDGAQLFQEFQRRGRVGSMQNVGKQIFDLLASEFAGVPQQQGPGGFETPA
                     GAGIGGLPATQLTPEQGLAAARAFLGFAMSWAIREGNQAMLAGLIPFGLGEHFRDYGT
                     DLARNIGLGRLTRIVFRPLFQAMVATPAEWAMNKQYRPKLLAPGDAWRGFNAGFLDGP
                     QLLEEFARQGYDETRRGVVQLLNTRMPNELQLFILELTGQMQRGEREIWLKRMGYTEE
                     TAGLVLKAQEKDFIRRLSFELAHRLLALVLSGAIDADTYKSIIDRFTFTESEKTALVG
                     MVSELLSHPTRRITLAQMESAFKQGVVTVVELEQYLQTRGYPEDDRGILLQLALLDLA
                     KVEEAAKVKAAREAAKKPTTGGSAGTPTG"
     gene            1736..2005
                     /locus_tag="AC16CX_12860"
     CDS             1736..2005
                     /locus_tag="AC16CX_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013438.1"
                     /translation="MLLDSVTYAALVVGIWLFVLLMPLLLFLAVRFLWYRHVYLGHSR
                     AVEELVHELRRIARALEERRAADAPAPKPDGAAVEHHVANSMFGR"
     gene            complement(2002..2829)
                     /locus_tag="AC16CX_12865"
     CDS             complement(2002..2829)
                     /locus_tag="AC16CX_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013439.1"
                     /translation="MPNGDGFGFGFDPSAFFAGILAAIEAVINAIISALVFLFNLLVF
                     AANFLLNGLLTVAKYLLDGFKVLLRGLKHVLSDIFHGRFKHLFEDYLKLKETLKRWFG
                     PLLKWLQRIRKWFDLYVLRPMKIIVNIIQRIRQILVIFRIFHFKFAEKLDAFLARVES
                     KLVKNVLFLRKVLNQVISLIDILVDPSLIFRRNVVGATLLSWLGAVKRIVGFGSNRAL
                     GADEVDSIEKDKALLKPGTTLVTRTGNSDPVFHPALARIMENIDRESKDFLPSGAGG"
     gene            complement(2833..3057)
                     /locus_tag="AC16CX_12870"
     CDS             complement(2833..3057)
                     /locus_tag="AC16CX_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013440.1"
                     /translation="MDAMVKIINVKDAVGIDPRTATPIRDKEITYTVGTHGPFTLRVH
                     ETDYNDANVTAQIEQNVATLRAIGAIPASG"
     gene            complement(3085..3873)
                     /locus_tag="AC16CX_12875"
     CDS             complement(3085..3873)
                     /locus_tag="AC16CX_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013441.1"
                     /translation="MATQPLIPLITDADLDLEPLTPDFLSTATSLIGNAGDDTDGFPS
                     LLLDLASSFDNMETDLAAGDPEIFDFLSIAGEVNPSSLDSDALTLGDSIAAGNAVLGD
                     FENLVDTGGGPTPPTGEGPLVPIEAATGPLRACGVTFAFPSASVQPAPVTRRLQFRNV
                     GTTPLRISRVTLVQADPTVITATTNLHETLPAGGAAVFTLTLTRDVPDGTTAQLVVET
                     TGPDSPNVICFTVGGGGTGGGGGGGGGGGGGEFGGIDSGGIQRR"
     gene            complement(4072..4263)
                     /locus_tag="AC16CX_12880"
     CDS             complement(4072..4263)
                     /locus_tag="AC16CX_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013442.1"
                     /translation="MNWKKILAFAASGAAAGSLGHYVTEIQQGHQMAFTAGNIVVPMV
                     TSLASTLLALFVKPPQDGK"
     gene            complement(4692..4967)
                     /locus_tag="AC16CX_12885"
     CDS             complement(4692..4967)
                     /locus_tag="AC16CX_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013443.1"
                     /translation="MDDGNPTRKDNGNGEHYTQLVLTLDVLPTWHLHIGGNVENADVA
                     VAMLDQAKRYFEGQLRAAAAMQLQRQMVRAAEDARIAAAVRGGKAPA"
     gene            complement(4979..5839)
                     /locus_tag="AC16CX_12890"
     CDS             complement(4979..5839)
                     /locus_tag="AC16CX_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013444.1"
                     /translation="MRPEEFKSASIPNVVSFALQGITPPSPLYIQRDDQLQVYAFTSV
                     AGDVLTVTARLLQADTNRITTIQFQVVLNATYSAIGTQRALAEGYLLSLTILSAAATQ
                     SGQTHVTALISRGLETIPPVVPGQILFSGYVTDRFLAAWPGGLFMQPTDGPGNPRVIT
                     IANPAAGADWTQTVQVVTNWELVCFNAVLTTSAAVANRQPQLALDDGATAYFTGPPVS
                     SLVASTTNTVSGAQTSGPASVITTDLMLPIPPGNFLRGGDRIRVVTAGIQAADQWSAI
                     RLLVREWLQI"
     gene            complement(5979..6842)
                     /locus_tag="AC16CX_12895"
     CDS             complement(5979..6842)
                     /locus_tag="AC16CX_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013445.1"
                     /translation="MPRAMEVVAGFATNPGATLTTLTPSTGTSFTVRGTDASKGTWLL
                     SAWAFNATAGEQRITSPKLHDQVQGIRNRITAAATFPLYAGPSDALFAQRLYAQDNIT
                     VQQSGGAAELDCGALLIAYDDLQGAAGRFIDFPTLKKIGQNVVTIEVTVTAVATGNFG
                     GAVAVNSTFDNLIANTDYAILGGLTDTRGCAVGITGIDFANLRVGFPAEPTLRELTNH
                     WFMALSVSANAPYIPVFNSANKAATTVDVETNGAGGTFIINLECVQLAPGAVGPAATG
                     ATVAGAAQPGA"
     gene            complement(6853..7203)
                     /locus_tag="AC16CX_12900"
     CDS             complement(6853..7203)
                     /locus_tag="AC16CX_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013446.1"
                     /translation="MATPQEPDFPPGHPARFDYNPASPEAKEWARIHVHPKGERDWPV
                     DHPKAVDTQGNKNAQVIEPGVDPDHPELEAFTGRTPEQAAAVKAFNAELAKVAKESPA
                     LEPVIAPPPPAPQP"
     gene            complement(7248..7670)
                     /locus_tag="AC16CX_12905"
     CDS             complement(7248..7670)
                     /locus_tag="AC16CX_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013447.1"
                     /translation="MAIQIKPIAAIADKFVKRAGAAGADYASGVQNPRTDQATAAAAA
                     APVWAQAVSDAAARDAFAKGVRAAGSAKWMDKAKSIGAQRYPQGVQAAKADFEAGFAP
                     YAQVLAGLTLQPRGIKGTNVGRVQQVVDALRKAKTGAA"
     gene            8146..8364
                     /locus_tag="AC16CX_12910"
     CDS             8146..8364
                     /locus_tag="AC16CX_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3013448.1"
                     /translation="MRETYAGRFVKRGNSHEVLIPKPVMERLRLTPGDLVVMRLFGPL
                     LIMRRLAPEMVVDVNEIPADALPSAARS"
     gene            8372..9247
                     /locus_tag="AC16CX_12915"
     CDS             8372..9247
                     /locus_tag="AC16CX_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3013449.1"
                     /translation="MAEYGQIPLEQVLEPPDPIRVSMDEKKLEELASDIRFQGVLQPI
                     IVVAEMRATGEHLTGPRDGTEEEVLEPTGRFEILAGHRRFLASRMAGLKQIPALIYPT
                     GEVAKEAVMLAENVCREDITAAEEGWLFCQMAEKYQLNEEQLCARVGRRPEYIYARMD
                     LVRKDAELAKLVAERKINFSVANALLKVSDEAHRRYLAQMAADSGCSTRVANMWVEQW
                     KAQQQVTAQSATASPSADTGSRPVEDPFKCFCCGKGGDPQNLRMLYVHWYELETWAKI
                     LREAGMEVDMSAVAK"
     gene            9254..9892
                     /locus_tag="AC16CX_12920"
     CDS             9254..9892
                     /locus_tag="AC16CX_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013450.1"
                     /translation="MAVEPRRGCGYRKVGGLYLVCKGSGMPCDRLPIPLTVCPTCSAG
                     FKQTRGWTWINVVGLTGGVHRNCADQFPCPLCMATSDMGRAGLLWIGERFYATPAAFT
                     YEADQLGISRRVKAVPRGFELGKTWVLLAHPKAITLADVEGDDALHYAPGIFRVFKPT
                     AVEKLITGSQSRDATVTAELQKQGITPVVVPDGDRDHQGTVYDKFEEQELFT"
     gene            9898..10080
                     /locus_tag="AC16CX_12925"
     CDS             9898..10080
                     /locus_tag="AC16CX_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013451.1"
                     /translation="MAEKRIPAAKPTAELTELYTQQPLVPDGDKVTRFETRFGKIFER
                     VESPLRGVSWFQVVQQ"
     gene            10077..10772
                     /locus_tag="AC16CX_12930"
     CDS             10077..10772
                     /locus_tag="AC16CX_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR3013452.1"
                     /translation="MSDKLTREQILEKVKNKESLSDADLSGADLSGADLSDAYLRGAY
                     LSDADLSGANLSDAYLSGAYLSGAYLRGAYLSGADLSDAYLSGAYLRGAYLSGADLSD
                     AYLRGANLRGANLRGAYLSDAYLSGADLSDADLSDADLSGAYLSGAYLRGANLQDIKH
                     NWLSHDLIAELLRRAARDDQELAVAGFVLMRREWCWQKFLECDLKGKLWALSVLRDLK
                     CPHVPTEPVEATT"
     gene            10769..11047
                     /locus_tag="AC16CX_12935"
     CDS             10769..11047
                     /locus_tag="AC16CX_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013453.1"
                     /translation="MIALLIWLAGCAFLGWVVWHWNLWQQLFGLVVWTAVFAWVVGGA
                     SWLGKHLGASAMRRGAVASSNPLIDALNRAAKRAGEKPASHAEPQQPQ"
     gene            11311..11553
                     /locus_tag="AC16CX_12940"
     CDS             11311..11553
                     /locus_tag="AC16CX_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013454.1"
                     /translation="MTKKKRRSTEGAVESAAERVAEQNGSIGAFRKATESWREERRGY
                     MAIKRACQELQRVGVGDPANLDKFVERVFRSAGVPQ"
     gene            11554..11802
                     /locus_tag="AC16CX_12945"
     CDS             11554..11802
                     /locus_tag="AC16CX_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013455.1"
                     /translation="MKDRLTTAIETVTGARQAVDAALMECSKYPKLALLSRPLLAASW
                     ALRSADFATRLAKSQLDELEYSQHSSEAAAPKPRGAAP"
     gene            11799..12014
                     /locus_tag="AC16CX_12950"
     CDS             11799..12014
                     /locus_tag="AC16CX_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013456.1"
                     /translation="MMDLDFIVQIIEDVKERYQREEEQAILQRNMDKAIAALAGKDAC
                     ARLFQEIMSRKAMFDNIERAQQRRKAG"
     gene            12236..12433
                     /locus_tag="AC16CX_12955"
     CDS             12236..12433
                     /locus_tag="AC16CX_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013457.1"
                     /translation="MASPTGIIPEEYFYPRRLGDVMEYLKKLPMPGDEKEKLFVGWAR
                     WVGVKINGSQRAAVRNSGIDR"
     gene            12653..13183
                     /locus_tag="AC16CX_12960"
     CDS             12653..13183
                     /locus_tag="AC16CX_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013458.1"
                     /translation="MATLAQRMEADRRKYPALLPDEVLVLRAWLRLHELEYDTLPLWW
                     LEREAAYPPPHPLITDRFDYNVRIGQGTDPGPSFTPNIRRDNILNTQLRLDALAFRGG
                     VPTIIEVKRRAGPSNVGQLLTYGAAWRQEFTTRPDPHLILLCADCGSNILPIVREAGI
                     DLQKVQVDFSPLKVPR"
BASE COUNT         2386 a         4421 c         4245 g         2482 t
ORIGIN      
        1 cgcgggcgcg tcgagttctt ctcctgggat ttgaattggt ttgtgtttgg gtttgttaac
       61 gttggatctc atgcagttct gagaatttcg ttacgaatgt ggacaaaatg gctcgttcag
      121 tgcaggtgga aaggtgtttc gaagcgtcaa aaatgcaggc ggcggcgcag ccttgcacga
      181 ggagtgcgcc caagctgctg ggggcgtagg accgcgcgac gccgtccgtg cctatcctgt
      241 aggcgtgccg gccgagccgc ctgtggttgg cttcttcgct gcctcgcgtg ctgccttaac
      301 cttcgccgct tcctcaacct tcgccaagtc caggagggcg agctgtagca ggatgccgcg
      361 gtcgtcctcg gggtacccgc gggtttgcag gtactgctcc agctcgacca cagtcaccac
      421 gccctgtttg aacgcgctct ccatctgcgc gagcgtgatc cgccgcgtcg ggtgggacag
      481 cagctcactg accatcccca ccagcgccgt tttctcggac tccgtgaacg tgaagcggtc
      541 gatgatcgac ttgtaggtgt cggcgtcgat ggccccggac aacaccaggg cgagcagccg
      601 gtgcgccagc tcgaagctga ggcgccgaat aaaatccttc tcctgcgcct tcagcaccag
      661 cccggcggtc tcttccgtgt agcccatgcg cttgagccag atctcccgct cgccgcgctg
      721 catctgcccc gtgagttcca ggatgaagag ctgcagctcg ttcggcatcc gcgtgttcag
      781 gagctggacc accccgcggc gggtttcgtc gtagccctgg cgagcaaact cctcgagcag
      841 ttggggtccg tcgaggaacc cagcgttgaa cccacgccaa gcatcgcccg gagccagcag
      901 cttcggccgg tactgtttgt tcatcgccca ctcggccgga gttgcaacca tcgcctggaa
      961 cagcgggcga aagacaattc gcgtcagcct tccgagcccg atgttccgcg ccaggtccgt
     1021 cccgtagtcg cggaagtgct cgccaagccc gaagggaatc agcccggcga gcatcgcctg
     1081 gttgccttcg cggatcgccc agctcatcgc gaagcccagg aacgcccgcg ctgccgcgag
     1141 tccctgctcg ggcgtgagct gcgtcgctgg cagcccgccg atgccagcgc cggccggcgt
     1201 ctcgaatccg ccaggtcctt gctgctgggg cacgccggca aactcactgg ccagcaggtc
     1261 gaagatctgc tttcccacgt tctgcatcga ccccacgcgc ccgcgccgct gaaactcctg
     1321 gaagagctgc gcgccgtcca cgtgcacgcc ggtgagatcc tcgatcaccg ccgccagcag
     1381 ctcataaaac tccgggctgc tctccgcctc ggccttgtcg atgaagtggg caatcgcgga
     1441 cagcagcttg tcggccgcgc gcgccgcgag ctcgatcagc gggtcgagca gccggataag
     1501 cgccgtccac agcgccgcgg caagccgctc gatccagccg gagtctttcg acccgagcgc
     1561 ctgcagctcg ccctgcttcc tgtagtccac ctcagagtcg gccacgtccg tgaccgtcac
     1621 cttgatgggc ggtcccaccg gctcaggggg tggttcggtt ccgttcggca cctattgcct
     1681 ccgctctatc tttcctggta cgctatccgc aaggtcccca tcgagaggtg cccccatgtt
     1741 gctagattcg gtcacctacg cggcccttgt ggttggcatc tggcttttcg ttctgctcat
     1801 gcctctgttg ctcttcctgg ccgttcgctt cttgtggtat cgccacgtgt acctcggcca
     1861 ctcccgggct gtcgaggagc tggtccatga gctgcggcgc atcgcgcgcg ccctggaaga
     1921 gcgccgcgcg gccgacgcgc cggccccgaa gcccgacggc gccgccgtcg agcatcacgt
     1981 cgccaactcc atgttcggcc gctaaccacc ggcgccgctt ggcaggaagt ctttcgactc
     2041 cctgtcgatg ttctccatga tccgcgcgag cgccgggtga aacaccgggt ctgagtttcc
     2101 ggtccgtgtc accagtgtcg tccctggctt cagtagcgcc ttgtccttct cgatgctgtc
     2161 cacctcgtcc gcgccgagcg cccgattgct cccgaacccg acgatgcgct ttaccgctcc
     2221 cagccatgag agcagcgttg ccccgacgac attccgccga aagatcagcg atgggtccac
     2281 caggatgtcg atcagcgaga ttacctggtt gagcaccttc ctaagaaaca atacgttctt
     2341 cactagcttg ctctcgactc gcgccaggaa ggcgtcaagc ttctccgcaa acttgaagtg
     2401 gaagatgcgg aagataacca ggatctgcct gatccgctgg atgatgttga cgatgatctt
     2461 catcggccgc agcacgtaca ggtcaaacca cttccggatg cgctgcagcc acttcagcag
     2521 cggcccaaac caccgcttca gcgtctcctt gagcttcagg tagtcctcga acaggtgctt
     2581 gaaccgcccg tggaagatgt ccgagaggac gtgcttcaga ccgcgcagca gcaccttgaa
     2641 gccgtccagc aggtacttcg ccacggtcag caggccattc agcaggaagt tggccgcgaa
     2701 caccagcagg ttgaacagga acaccagggc cgagatgatg gcgttgatca ccgcctcgat
     2761 ggcggcgagg atcccggcga agaaagcgct ggggtcgaag ccgaagccga acccgtctcc
     2821 gttcggcatg ggttagccgc tcgcggggat agccccgatg gcgcgcagcg tcgccacgtt
     2881 ctgctcgatc tgcgcggtca cgttggcgtc gttgtaatcg gtctcgtgca cgcgcagggt
     2941 gaacgggccg tgcgtgccca ctgtgtaggt gatctctttg tcgcggatcg gcgtcgccgt
     3001 cctcgggtca atgcctaccg cgtccttcac gttgatgatc ttcaccatcg cgtccatggc
     3061 tatctccccg cccgcagctc gccactaccg ccgctgaata ccgcctgagt caattccacc
     3121 aaactcgccg ccaccgccgc cgccaccacc gccaccgcca ccgcccgttc cgccacctcc
     3181 caccgtgaag cagatcacgt tcggcgaatc cggcccggtc gtctcgacca cgagctgcgc
     3241 cgtggtgcca tcaggcacgt cgcgcgtcag cgtcagtgtg aacacggccg cgccgcccgc
     3301 cggcagcgtc tcgtgcaggt tcgtcgtcgc ggtgatcacc gtcgggtcgg cttgcacgag
     3361 tgtcaccctc gagattcgca gtggcgtcgt gccgacgttg cggaactgca gcctgcgcgt
     3421 gaccggcgcc ggctgcacgc tcgcgctcgg aaacgcgaag gtcaccccgc aggcgcggag
     3481 cggtcccgtc gcggcctcga tggggaccaa tggaccttca cccgtcggcg gtgttggccc
     3541 gccacccgtg tcgactaggt tctcgaaatc gcccaggacg gcgttgcctg cagcaatcga
     3601 gtcacccagc gtcagcgcgt cggagtcgag cgagctgggg ttcacctctc ccgcaatgga
     3661 caaaaagtca aagatctcgg gatctcccgc ggccaggtcc gtctccatgt tgtcaaacga
     3721 gctggcgaga tccagtagaa gggaagggaa gccgtcggta tcatccccgg cgttgccaat
     3781 caaactcgtg gctgtgctga ggaagtcggg cgtgagcggt tcgagatcca gatccgcgtc
     3841 ggtgatcagc gggatgagcg gctgtgtcgc catcgtgcct cacgcttgct cgtcctcgtc
     3901 gtcctcttcg tcgtcgtcgt cctcgagttc aagctgtgtt tgccaccgct cccggccctt
     3961 ccggaaccac gaaagcacca tctccaccaa aaatgccacg tggtacccct ttgggccgtt
     4021 gcgttcgtcg ctctacgcgc ctcgcaaagc ccaaaaccgc ccctcgcgag gtcacttccc
     4081 gtcttgcggc ggcttcacga acagcgccag cagggtcgag gctagcgaag tcaccatcgg
     4141 caccacgatg ttccccgcgg tgaacgccat ctgatgcccc tgctgaatct ccgtcacgta
     4201 gtgccccagc gagcccgctg cggcgcccga cgccgcgaaa gccagaatct tcttccagtt
     4261 catcgcttcc gcctttccct ctctctcaca gcgcaaggac taggatcgag acatcctgaa
     4321 cggtgacggc ggtggctgcg ttgctgatgt tacatgagag cgagtactgc aaatttgcca
     4381 cgatccccgg cgagtcgagc gtattgccgg accgctggta ccgcacagag gccacctcgg
     4441 tacacacttg gcctgatgtg atcaacgtgc tcgacgtaag cggcccccgc cggatgttgt
     4501 acgcaatctg cggaccgacg gcacccccca agtacgtgat gctccaaatg atgaggaaca
     4561 cctgcccgtc gattgactgg ttgatgggcg gtgacgttgc cgctatccac tcggagggcg
     4621 gggcactcgg agttccagcc gtcgttgacc ctacacttgg aaacctaact cccatcactc
     4681 acctcccgca tctacgccgg cgctttgccg ccgcgcaccg ccgcggcgat ccgcgcatcc
     4741 tcagccgcgc gcaccatctg ccgctgcagt tgcatcgccg ccgcagcgcg aagctgtcct
     4801 tcgaagtagc gctttgcctg gtccagcatc gccacagcca cgtcggcatt ctcgacgttt
     4861 ccaccgatgt gcaggtgcca cgtcggcagc acatcgagcg tcagcacgag ctgcgtgtag
     4921 tgctcgccat tgccgttgtc cttccgcgtt gggttgccat catccatcgc ctgcctcact
     4981 agatctgcag ccactctcga acaagcaacc gaatcgcgct ccactggtcc gccgcctgaa
     5041 tcccggcggt caccacacgg atgcggtcgc cgccgcgcag gaagttccct ggcgggatcg
     5101 gcagcatcag gtccgtggtg atcaccgacg ctgggcccga agtctgcgcg ccactcaccg
     5161 tgttcgttgt gctcgccacc aggctcgaca caggcgggcc tgtgaagtac gcagtcgcac
     5221 cgtcgtccag cgcgagctgt ggctggcgat ttgctaccgc ggcgctcgtc gtcaggacgg
     5281 cgttgaagca caccaactcc cagttggtga ccacctgcac cgtctgcgtc cagtcggcgc
     5341 cggctgcagg attcgcgatg gtgatcacgc gcggattccc agggccgtcg gtcggctgca
     5401 taaacagccc gcccggccat gcagcaagaa atcgatctgt gacgtacccg gagaagagaa
     5461 tctgccccgg caccactggg gggatggttt caagcccgcg actgatcagc gccgtgacgt
     5521 gtgtttgtcc gctctgcgtc gcagctgcgc tcaggatggt gagcgatagt agatagccct
     5581 cggccaatgc gcgctgcgtc ccaatggccg agtacgtggc gttcagtacc acctgaaact
     5641 gaattgtggt gatgcggttc gtgtcggctt gaagcaggcg cgccgtcaca gtgagaacat
     5701 cacccgcgac tgaggtgaaa gcgtacacct ggagctggtc gtctcgctgg atgtacagcg
     5761 gggaaggcgg agtaatgcct tgcagggcaa acgagacgac gttaggaatg cttgccgact
     5821 tgaactcttc cggcctcatg ggcgtcggct gccctgcgcc cggaagtggc ggaatgccac
     5881 cgctgcccat tcgctcagct cctaagagaa aaggccgagc cgacgttcat cagcccggcc
     5941 tcttccgccc gatcccgctc cgaggaagga aacggtcgtt acgctcccgg ctgtgcggcg
     6001 cccgccaccg ttgcgcccgt cgctgccggg cctaccgcgc ccggtgcgag ctgcacgcac
     6061 tcgaggttga tgatgaacgt tccgccagca ccgttcgtct ccacgtccac cgtagtcgct
     6121 gccttgttgg ccgagttgaa cactgggata tacggcgcgt tcgcggaaac cgacagcgcc
     6181 atgaaccagt ggttggtcag ctcgcgcagc gtcggctcgg cagggaagcc cacgcgcagg
     6241 ttcgcgaagt cgatccccgt gatgcccacg gcgcagccgc gcgtgtcggt cagcccgccg
     6301 agaatggcat agtccgtgtt cgcgatcagg ttgtcgaacg tgctgttcac cgccacggcg
     6361 ccgccgaagt tgccggtagc gaccgcggtg accgtcacct cgatggtcac gacgttctgc
     6421 ccgatcttct tgagcgtggg gaagtcgatg aatcgccctg ccgcgccctg caggtcgtcg
     6481 tacgcaatca gtagggcgcc gcagtcaagc tccgcagcgc cacctgactg ttgcaccgtg
     6541 atgttatcct gggcgtacag tcgctgcgcg aataacgcgt cgctcgggcc tgcatagagc
     6601 gggaatgtgg cggccgcggt gattcggtta cgaatgccct gcacctggtc gtgcaatttc
     6661 ggagaggtga tgcgttgctc acccgccgtg gcgttgaatg cccacgcaga gagcagccag
     6721 gtgcctttgc tcgcgtcggt gccgcgcacc gtgaaagacg ttccagtgct cggcgtcaag
     6781 gtcgtcagcg tcgcgccggg gttggttgcg aatcctgcca ctacttccat agctcgcggc
     6841 atgggtctct cctcacggtt gcggcgctgg cggaggcgga gcaatcaccg gctcgagtgc
     6901 cggagattct ttcgccacct tcgccagctc cgcgttgaat gccttcactg cggcggcttg
     6961 ctccggggtt cgcccagtga acgcctcgag ctccgggtgg tcgggatcca cccctggctc
     7021 gattacctgc gcgttcttgt tcccctgtgt gtccacagcc ttcggatggt caaccggcca
     7081 gtcgcgctcg cctttcgggt gcacgtggat acgcgcccac tctttcgctt ccggggacgc
     7141 cggattgtag tcgaatcgcg ccgggtgtcc tggcgggaag tcgggttctt ggggtgtcgc
     7201 catgcgcctg ccttcgtttc gtgctcagtc caccactcgc ggggcaatta agccgcgccc
     7261 gtcttggcct tgcgcagcgc gtccaccacc tgctgcaccc ggccaacgtt cgtgcccttg
     7321 atcccgcgcg gctgcaaagt aagccccgcc aggacctgcg cgtagggcgc gaaccccgct
     7381 tcgaagtcgg ccttcgccgc ctgcacgccc tgcggatacc gctgtgcccc gatggacttt
     7441 gccttgtcca tccacttcgc gctcccggcc gcacggactc ctttggcgaa agcgtcgcgc
     7501 gccgcggcat cgctcacggc ttgtgcccaa acgggcgccg ccgcagcggc cgcggtggct
     7561 tggtccgtcc gcgggttctg cacgccgctg gcatagtccg ccccggccgc gccagcacgc
     7621 ttcacaaact tgtccgcaat cgcggcaatt ggcttgatct ggatcgccat ctcaaccctc
     7681 cgcccgcgtg tgcttcgcga gccactgtgg aagaaactgt ggaaactgtg agtatttccg
     7741 gtgcacgact gcggaaagct cacccacgac gagccgaact tcacagacga aaaacacttg
     7801 tcaagagaat tctcacggga aatccacagg tgggcagggc agtggaatca gttagttgca
     7861 gggcgtctgt agggtggtga ttttaggggt taaatgcttg caggtcagtt ggttgacata
     7921 atataacgtt gtcggttctc tagcctgaat tccaccgcac tcatcatcac aacaatcttc
     7981 tacgcatagg taaatacagt tgttgttgtg gaacccatgg tagggtgcgc acagtgtcat
     8041 caggtatggg tgggcgcgtg gcggcgcgat ggagcacgtc ccaaggtgtt acgcctctag
     8101 agagctcgtg gtcgtcaccc ccacccagga tgctactgag gaaccgtgcg agagacgtac
     8161 gccgggcgat tcgtgaagcg tggcaacagc cacgaggtgc ttatccccaa gccggtgatg
     8221 gagcgcctgc ggctgacacc tggcgacctc gtcgtgatgc ggctcttcgg cccactactg
     8281 atcatgcgcc ggctcgcgcc ggaaatggtg gtggatgtga atgagatccc agccgacgcc
     8341 ttaccgtcgg cggcgaggag ctgagcccgc cgtggctgag tacggccaaa tcccactcga
     8401 acaggtcctc gagccgcccg atcccatccg cgtctcgatg gacgaaaaga agctcgaaga
     8461 gctcgcctcc gacattcgct ttcagggcgt gctccaaccc atcatcgtcg tggcggaaat
     8521 gcgcgccacc ggcgagcacc tgacgggccc gcgcgacggc acggaagagg aagtgctgga
     8581 acccaccggc cgcttcgaga tccttgccgg ccaccgccgc ttcctcgctt cccgcatggc
     8641 cggactgaaa cagatccccg cactgattta ccccaccggc gaagtcgcga aggaagcggt
     8701 gatgctcgcc gagaacgttt gccgcgagga tatcaccgcc gccgaggaag gctggctgtt
     8761 ttgccagatg gccgagaagt accagctcaa cgaggagcag ctctgtgctc gtgtcggccg
     8821 tcggcccgag tacatctacg cgcgcatgga cctggtgcgc aaggatgccg agctggccaa
     8881 gctcgtggcc gagcgcaaga tcaacttcag cgtcgccaac gccctgctca aggtatccga
     8941 tgaagcccac cgccgctatc tcgcgcagat ggccgcggac tccggctgct ccacgcgcgt
     9001 ggccaacatg tgggtcgagc agtggaaagc tcagcagcag gtgaccgcgc agtcggcgac
     9061 ggcgagtccc tcggccgaca ccggctcacg acccgtcgag gaccccttca agtgcttctg
     9121 ctgcggcaag ggcggcgacc cgcagaacct gcggatgctc tacgtccatt ggtacgagct
     9181 cgaaacctgg gccaagattc tccgtgaggc ggggatggaa gtggacatgt cggcggtggc
     9241 gaagtgacac caaatggcgg ttgaacctag gcgcggctgt gggtaccgca aggtcggagg
     9301 gttgtacctc gtctgcaagg gcagcgggat gccgtgcgac cggctgccca tcccactcac
     9361 cgtttgcccg acgtgctcgg ccgggttcaa gcaaacccgt ggatggacct ggattaatgt
     9421 tgtgggcctg acgggtggcg tgcatcgcaa ctgcgcggac cagtttccgt gtccactttg
     9481 catggcgacg agcgacatgg ggcgcgctgg ccttctatgg atcggggaga ggttctacgc
     9541 tacgccggca gcgttcacct acgaggcgga ccagctcgga attagccggc gcgttaaggc
     9601 cgtgccgcgc ggcttcgagt tgggcaagac ctgggtactt cttgctcacc cgaaggccat
     9661 cacgctcgct gatgttgaag gcgacgacgc tctgcactac gcgccgggta tcttccgcgt
     9721 gttcaagccc accgccgtcg agaagctcat caccgggagt cagagtcgcg atgctacggt
     9781 aaccgcggag ctgcaaaagc agggcatcac tccggttgtc gtaccggacg gcgaccgcga
     9841 ccaccagggt acggtgtacg acaagttcga agagcaggag ctgtttacat agccccgatg
     9901 gctgagaagc gcatcccggc agcaaagccc acggcagagc tcaccgagct ctacacccag
     9961 cagccgctgg tgccggacgg tgacaaagtg acgcgctttg agactcgctt cggcaagatc
    10021 ttcgagcggg tggagtcgcc gcttcgcggc gtctcctggt ttcaggtggt gcagcagtga
    10081 gcgacaaact gacgcgcgag caaattctcg agaaggttaa gaacaaagaa tccctgagcg
    10141 acgcggacct gagcggcgcg gacctgagcg gcgcggacct gagcgacgcg tacctgcgcg
    10201 gcgcgtacct gagcgacgcg gacctgagcg gcgcgaacct gagcgacgcg tacctgagcg
    10261 gcgcgtacct gagcggcgcg tacctgcgcg gcgcgtacct gagcggcgcg gacctgagcg
    10321 acgcgtacct gagcggcgcg tacctgcgcg gcgcgtacct gagcggcgcg gacctgagcg
    10381 acgcgtacct gcgcggcgcg aacctgcgcg gcgcgaacct gcgcggcgcg tacctgagcg
    10441 acgcgtacct gagcggcgcg gacctgagcg acgcggacct gagcgacgcg gacctgagcg
    10501 gcgcgtacct gagcggcgcg tacctgcgcg gcgcgaacct gcaggacatc aagcacaact
    10561 ggttgagcca cgacctcatt gctgagctgc tgcgcagggc cgcgcgagac gaccaggaac
    10621 ttgccgttgc ggggttcgtc ctcatgcggc gcgagtggtg ctggcagaaa tttctcgagt
    10681 gcgacctgaa ggggaagctg tgggcgctgt cggtgcttcg cgacctaaag tgcccgcacg
    10741 tgccgacgga gccagtcgag gcgacgacat gatcgcgctg ctcatctggc tggccggctg
    10801 cgccttcctc ggctgggtgg tgtggcactg gaacctctgg cagcagctct tcggcctggt
    10861 ggtttggacg gcagtgttcg cctgggtagt cggaggagca tcgtggctcg gaaagcatct
    10921 cggcgcgtcg gccatgaggc gcggcgccgt cgccagcagc aacccgctca tcgacgcgct
    10981 caaccgcgcg gcgaaacgtg cgggagagaa gccggcgtcg cacgcggagc cgcagcagcc
    11041 gcagtagctc gcgctctcgg cgaagctgcg ccggccgtgc aagttgaggc ggggcgagtc
    11101 ctgtgtccac actgccgcca accgatggaa cgctgcgagc tgcagatcgg agtggacttg
    11161 ccacacttcc gcagcaggga gtgcggagtg atttggagcc tgcacctggg gaaactctcc
    11221 ccgtgcgtga ccccggagct gcacgtgaag ccagtgacca agatctcggg ccagcctacg
    11281 ggctaactca cgacctagcg gagtgaaacc atgaccaaga aaaagaggcg cagtacagaa
    11341 ggtgcagtcg agtcagcagc ggagcgcgtc gcggagcaga acggctcgat tggagccttc
    11401 cgcaaggcga cggagagctg gcgcgaagag cgccgcggct acatggcgat caagcgcgcc
    11461 tgtcaggagc tgcagcgcgt cggcgttgga gatcccgcca acctcgataa gtttgtcgag
    11521 cgcgtgttcc gttccgccgg cgtgccgcag tagatgaagg accggctcac caccgcgatc
    11581 gaaaccgtca ctggcgcgcg ccaggccgtg gacgcggcgc tcatggagtg cagcaagtac
    11641 ccgaagctag cgctgctctc ccggccgctg ctcgccgcct cgtgggcgct gcgctcggcg
    11701 gacttcgcaa cgcggttagc aaagtcgcaa ctggacgaat tggagtacag tcagcactct
    11761 tccgaagccg cagccccaaa accacgaggt gctgcgccat gatggatcta gacttcatcg
    11821 tacaaatcat cgaggacgtg aaggaacgct accagcgcga agaggagcag gccattcttc
    11881 agcgcaacat ggacaaggcc atcgcagcgc tcgccgggaa ggatgcctgt gcccggctct
    11941 ttcaggagat tatgagccgc aaggccatgt ttgacaacat cgagcgggca cagcagagga
    12001 gaaaggccgg atagatgccg cggggtcctg ggggagcgaa tcaccggccg gtttggccgg
    12061 aaggcacggc ggtgactgaa gtcatggcct gggcgagtag ggtgctcagc aacgagctta
    12121 tcctgttcgc ggccgcagct tctgacggtg accgcgacct gatggccgtg cagtgccgcg
    12181 cgcttcgcgg cgtggtcacg cgcatcgacc agttccggcg aatgaaggtg cgacagtggc
    12241 gagcccaaca gggatcatcc ccgaagagta cttctatccc cgccgcctcg gcgacgtgat
    12301 ggagtacctg aagaagctgc ccatgccggg cgatgagaag gaaaagctgt tcgtcggttg
    12361 ggcgcgctgg gtcggggtga agatcaacgg cagtcagcgc gccgccgtgc gcaacagcgg
    12421 gatcgacaga tgagtatccc tattaagcct accagcccta ccttgccagg tccacctgca
    12481 atggaagaca cgctggtgct tacgctttcg gttaccccca agggcgaagt tgtttggtgg
    12541 atatcgagtc cgtttgcctt gcagatgaac cacggacttg ttgatgagct atccgccact
    12601 cttcgccact tcctgcagga tttcgtcagg gcgagagggt agaggaaagc gcatggcgac
    12661 actagcacaa cgcatggaag ctgaccggcg gaagtacccc gcgctgctgc ctgacgaggt
    12721 gctggtgctc cgcgcctggc tgcggctgca cgagctcgaa tacgacacgc tcccgctgtg
    12781 gtggcttgag cgtgaggcgg cttacccacc gccgcatccg ctcatcaccg accgcttcga
    12841 ctacaacgtg cgcatcggcc agggcacgga ccctggcccg agttttacgc caaacatccg
    12901 ccgcgacaac atcttgaaca cgcaacttcg cctcgacgcc ctggccttcc gcggcggcgt
    12961 gcccacgatc atcgaggtga agcgccgcgc cggcccgtcg aacgtcgggc agctcctcac
    13021 gtacggcgcg gcctggcgcc aggagttcac cactcgcccg gatccccacc tgatcctcct
    13081 ctgtgccgac tgcggctcga acatcttgcc gatcgtcagg gaagccggca tcgacctgca
    13141 gaaggtccag gtcgatttct cgcccctcaa ggtcccgcgg taggtgttac acttagccga
    13201 gtaggagagc gccccgttga gcagtgaagc gcacgagcct gcggcaatca agggtctaac
    13261 gtcggaccta gccaagagct acgagctcgc ctactggacg tgtcgtgtgt gtggcaaccc
    13321 gttcgcgatg gtgatcggcc acaggccaca cgcctgccct gtatgcggcg tcgcgtacac
    13381 acagcttcta atcgacggtg agtacgtaac acctggctag ggcggcagcc gcgtaggtcc
    13441 gagcacttct acggcaggac caacccgccg cttttttgac cttgaaggtt ttcgaccaac
    13501 tcctaccttc tcccaacgcg cggcgcccgc gttt
//
LOCUS       DBNGWZ010000429         6631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2319799, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000429 DBNGWZ010000000
VERSION     DBNGWZ010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2319799"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16CX_12965"
     CDS             <1..368
                     /locus_tag="AC16CX_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013459.1"
                     /translation="TAQEILKRQAESHRYRLLLAQSSQALLDHMRADNRTGAWVQEAV
                     KAKERVNKDYRGYNLAAGALDKLLESFPASQAKIMPYVGPPAIIPETLAGDARKRVQQ
                     DTRQVAAEFEKLQQLGALR"
     gene            complement(469..1182)
                     /locus_tag="AC16CX_12970"
     CDS             complement(469..1182)
                     /locus_tag="AC16CX_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3013460.1"
                     /translation="MSTEQSLRHDVALANRIIERFGLSNAFGHVSARIPGTSTFYIPT
                     RRSPGLADENNLLVIDTDGKIVSGDGTPNSEFWIHARIYAARPDVGGVVHAHPLACIA
                     LTQIGQPHRVVHNQGGAFADGVPEYERIGLIRSRELGDRVAQRLGGGVAVMMRGHGIS
                     TAFQDVRAATVAACFLEESAALQMRMLTAAGGDAARLRAFSREEAVLVREQTGPAGKI
                     SARAWEYYAMAAEQRPLGG"
     gene            1376..1975
                     /gene="pcp"
                     /locus_tag="AC16CX_12975"
     CDS             1376..1975
                     /gene="pcp"
                     /locus_tag="AC16CX_12975"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR3013461.1"
                     /translation="MKVLVTGFEPFGGESVNASLEAVRRLPQRIGALEIVTADLPASY
                     ARSIPALEAAIARSRPEIVLCVGQAGDRAALCVERVAVNLQDARIPDNDGAQPVDIPV
                     VAGGPAAYLSTLPVRAAVAALRAAELPAQLSMSAGTFVCNHVFYGLMHLAATRNHPFR
                     GGFLHVPALPRQPAGAASPVALDDIVRGIETVLESAAKA"
     gene            complement(2101..3660)
                     /locus_tag="AC16CX_12980"
     CDS             complement(2101..3660)
                     /locus_tag="AC16CX_12980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HIR3013462.1"
                     /translation="MQLLEVILFLMLCSVALGWCARHFRFPYPIALVIGGGALGFVPQ
                     LPQLPFDPQFILVLVLPPILYQAALLTSWRDFRANIRPIGLLAVGLVIATTLAVGAAL
                     KFLIPEVPWAAAFVLGAIVSPPDAVAATAILSRLSIPRRVVTVLEGESLVNDASGLVL
                     YKFAVAAVLTGAFSLLDASVQFVGVSIGGIVIGILMGLLFVFVHRHLGDAFIEVLMTL
                     SVPYITYIFAESLHASGVLAVVAAGLVRGRYAPEIVSAEMRIIARSVWNLLVFLLNSL
                     VFILIGLQLSDIATRLTGHSPAQLFLYGTLISVMAILVRFAWIYPAIYLPRMLSARLR
                     SREPPPPEAEVFIMSWCGMRGIVSLAAALALPLALADGAPFPQRDLIVFLTFVVIAVT
                     LVLQGLTLAPLIRRLRVGTDWSGQEEHLRARIALTKAAVAAIDGLAADEGIPETVAER
                     IRAEFAEKLALAVPGGVVMSGGADPARRLRRAAIEAERSELIRIWRDNQISDDVLHHL
                     EEDLDYQESRL"
     gene            complement(3758..3946)
                     /locus_tag="AC16CX_12985"
     CDS             complement(3758..3946)
                     /locus_tag="AC16CX_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013463.1"
                     /translation="MAKSNFRYEKRQRELAKKRKQEEKQQRKLEKNSPQPPEQGGQPP
                     DSPQSGGAGDGQSGPASQ"
     gene            complement(4022..4336)
                     /locus_tag="AC16CX_12990"
     CDS             complement(4022..4336)
                     /locus_tag="AC16CX_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013464.1"
                     /translation="MAPAVAQNEVFAALPGVGLKADDAMVLREEIGQYLIEASAWRPS
                     EKDKWRPRLTITRTGGGGPVQLSQTFRDSSAVFDEAEGARRNAYERGKRLVSEGPPNL
                     KV"
     gene            4417..4839
                     /locus_tag="AC16CX_12995"
     CDS             4417..4839
                     /locus_tag="AC16CX_12995"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3013465.1"
                     /translation="MKLSAKNPLPLASRSAATGVLVFALAGCGQEPPPQPAAKATPPA
                     APAAPKAEIKAEPAKPAAVESGQASADKTLAERVKAALLEDRSLKAHGVDVVVKDGIV
                     TLFGTTETPVRRELAGRIAAGVQGVKSVENKLAVVAGS"
     gene            5045..6559
                     /locus_tag="AC16CX_13000"
     CDS             5045..6559
                     /locus_tag="AC16CX_13000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3013466.1"
                     /translation="MTVAARHPVRVLLVNPRYPESFWSFQWALDRILRGKRAVNPPLG
                     LATIAALTPPHWRVEIVDENVEPVPAWPRADIVGVCGMAIQFPRQKELLERYRRAGYY
                     TVTGGSYASLCPEAYHDIADCVVAGEAEYVWREFCADWERGRLRSLYRETGVVSLEDS
                     PTPRFDLLRLERYTTATLQFSRGCPFRCDFCDIIVMFGRKPRYKTVRQIEAELDALRS
                     LGAGNVFFVDDNLIGHKAASKELLGFLAGYQAKHGRPFRFGAQVSINVAHDPAFLKLL
                     RAANFTWVFIGIESPDLEALKHTRKTQNTQQDLLTCVRRVYAHGIDVLGGFIVGFDTD
                     TPRAFDAQFQFIMRSGIQAAMVGLLTALPRTPLYMRLEKEGRLRAGAAASDNTKPATN
                     IIPKLMSYDELVEGYRALLAKLTQDRHIAGRIRNKLKYLHSPTYQGEYGWGERLAILW
                     RLLTRGVLPGGPRRILSFARSLPWFSPRKLPIAVMDWIAGLALRDFLVRRFPGQ"
BASE COUNT         1145 a         2263 c         2164 g         1059 t
ORIGIN      
        1 tgaccgccca ggaaatcctg aagaggcagg ccgagagcca ccgctaccgc ctgttgctgg
       61 cacaaagctc gcaggcgttg ctcgatcaca tgcgcgccga caaccgcacc ggtgcctggg
      121 tccaggaagc ggtcaaggcc aaggagcggg tcaacaagga ttaccgcggc tacaacctcg
      181 ccgccggcgc gctcgacaag ctgctcgaat cgttcccggc ctcgcaggcg aaaatcatgc
      241 cgtatgtcgg gccaccggcg ataatccctg aaacgctggc aggcgacgcg cgcaagcggg
      301 tgcagcagga cacaaggcag gttgcggccg aattcgagaa gctgcagcag ctcggcgctt
      361 tacgctgaga ccgaaaagcg cgggtcgtga gtggtgaata gtgaatgggc tttgttgcct
      421 tccgccttcc gccttccgcc ttccgccctc tgttctcctt cctcctggct agccgcccag
      481 cggtctctgc tcagcggcca tggcgtagta ctcccacgcc cgcgctgaaa tcttaccggc
      541 ggggccggtc tgctcgcgca cgagcacggc ttcctcgcgg ctgaaggcgc ggagccgcgc
      601 ggcgtcgccg cccgccgccg tcagcatgcg catctgcagc gcggcgctct cctcaagaaa
      661 gcaggccgcg accgtcgccg cgcgcacgtc ctggaacgcc gtgctgatgc cgtggccgcg
      721 catcatgacg gcgacgcccc cgccgaggcg ctgggcaacg cggtcgccca gctcgcgcga
      781 gcggatcagg ccgatgcgtt cgtactccgg aacgccgtcg gcaaaagcgc cgccctggtt
      841 gtgaacgacg cggtggggct gcccgatctg ggtgagggcg atgcaggcga gcggatgcgc
      901 gtggaccacc ccgccgacgt cgggccgcgc ggcgtagatg cgcgcatgga tccagaattc
      961 gctgttcggc gtgccgtcgc ccgacacgat ctttccgtcg gtgtcgatga cgagcaggtt
     1021 gttctcgtcg gcaaggcccg ggctgcggcg ggtcgggata tagaacgtgc tcgtgcccgg
     1081 aatccgcgcg ctcacgtgac cgaacgcgtt ggacagcccg aaacgctcga tgatgcggtt
     1141 ggcaagcgcg acatcatgcc gcagggactg ctcggtgctc acaatgatcc tcctgtatcg
     1201 atggaagcgg cgtcattctc ccacacccat gaggtgacgg agtgacgagt gtcgggtatc
     1261 gagtgaggag tgacgagtgt cgagtaacga gtatttaccc ccctcgaccc tccatcctcg
     1321 tccctcgttt cttccctcga cactcaatcc tcgacacttt attctcatcc ctcccatgaa
     1381 ggtcttagta acgggtttcg agccgttcgg tggcgaaagc gtcaacgctt cgctcgaagc
     1441 agtgcgtcgg ctgccgcagc ggatcggcgc gctggagatc gttacggcgg acctgccggc
     1501 ttcttacgcg cgctcgatcc ccgcgctgga agccgcgatc gcacgctcgc ggccggagat
     1561 cgtgctgtgc gtgggacagg cgggcgaccg cgcggcgctg tgcgtcgaac gggtcgcggt
     1621 caacctgcag gacgcgcgta tcccggacaa cgacggcgcg cagccggttg acattcccgt
     1681 cgttgcgggc gggccggcgg cctatctctc gacgctaccg gtgagagccg ccgtcgccgc
     1741 gctgcgcgcc gcggaactgc cggcgcagct gtccatgagc gccggcacct tcgtctgcaa
     1801 ccacgtcttc tacggcctca tgcacctcgc cgcaacgcgc aaccatccct tccgcggcgg
     1861 gtttctccac gtgcccgccc tcccccggca gccggcgggc gcggcctccc ccgtggcgct
     1921 cgacgatatc gtgcgcggca tcgagaccgt gctggaatcg gcggccaagg cctgactcgg
     1981 ccgccgatca atacaaatcc tgcatacgaa caaaaaaatt accgttaaga gtcgcgtttc
     2041 atcggtcgcg tatcacctgt tacacaatgt caggcgcgaa acgcccgcta tgccgtcaag
     2101 tcataagcgc gattcttggt agtcgaggtc ttcctcaagg tggtgcagca cgtcgtcgct
     2161 gatctggttg tcccgccaga tgcggatcag ctcgctccgc tccgcctcga tcgccgcgcg
     2221 tctcagccgc cgggcggggt cggcgccgcc gctcatcacg acgccgcccg gcaccgcgag
     2281 cgcgagcttc tcggcgaatt cggcccggat gcgctcggcc actgtttccg ggatcccctc
     2341 atccgccgcc agcccgtcga tggcggcgac cgccgccttg gtcagggcaa tccgggcgcg
     2401 cagatgctcc tcctgcccgc tccagtcggt gccgacgcgc agcctgcgga tcagcggcgc
     2461 gagcgtcagc ccctgcagca ccagcgtgac cgcgatcacc acgaaggtga ggaacacgat
     2521 gagatcacgc tgggggaacg gcgcgccgtc cgccagcgca agcggcagcg ccagcgcggc
     2581 cgcgagcgac acgataccgc gcatgccgca ccagctcatg atgaaaacct cggcctcggg
     2641 cgggggcggc tcgcggctgc ggagccgcgc gctcagcatg cgcggcaggt aaatggcggg
     2701 ataaatccac gcgaacctga cgaggatcgc catcacgctg atcagcgttc catagaggaa
     2761 aagctgcgcc ggcgaatgac cggtgagcct cgtggcgatg tccgagagct gcaggccgat
     2821 caggatgaaa accaggctgt tcagcaggaa cacgaggagg ttccagaccg agcgcgcgat
     2881 gatgcgcatc tccgccgaga cgatctccgg ggcatagcgc ccgcgcacca gccccgccgc
     2941 gaccacggcg agcacgcccg aggcgtgcag ggattcggcg aagatgtagg tgatgtacgg
     3001 caccgagagc gtcatcagga cctcgatgaa ggcgtcgccg agatggcggt ggacgaagac
     3061 gaacagcagc cccatcagta tgccgatgac gatgccaccg atcgaaacgc ccacgaactg
     3121 gacgctcgcg tcgagcagcg agaaggcgcc ggtcagcacc gccgcgaccg cgaacttgta
     3181 gagcaccagc cccgaggcgt cgttcaccag gctctcgccc tcgagcacgg tcaccacccg
     3241 gcgcgggatg ctgagccgcg acaggatcgc ggttgccgcc accgcgtcgg ggggcgagac
     3301 gatggcgccg agcacgaagg ccgcggccca gggaacttcc ggtatcagga acttgagcgc
     3361 ggcgccgacc gcgagcgtag tcgcgatgac gagcccgacg gcgagcaggc cgatggggcg
     3421 gatattggcc ctgaaatcgc gccacgaggt gagcagcgcc gcctggtaga ggatgggcgg
     3481 cagcaccagg accaggatga actggggatc gaacggcagc tgcggcagct gcggcacgaa
     3541 accgagcgcg ccgccaccga tgaccagcgc gatcggatag gggaaacgaa agtgccgcgc
     3601 gcaccagccg agcgcgaccg agcacagcat caggaacagg atgacttcca gcagttgcat
     3661 ggcgccccgc tcgaaaagcc gccattaaac catgcccggc ggtttttgat gaagcgtgac
     3721 gggtaacgag tgaccggcga cgggagaaaa ccaccggtca ctgcgaagcg ggaccggact
     3781 gcccgtcccc tgccccgccg gactgggggc tgtctggagg ctgcccgccc tgctccggcg
     3841 gctgtggact gtttttctca agcttgcgct gctgtttttc ctcctgcttt cttttctttg
     3901 ccagctctct ttggcgcttc tcgtatcgaa aattggattt tgccaatggg cacccttttc
     3961 tcgcggctgc tgtcgatgga aaaaccggcc ctaacctcgg agccgggtgt atcaggcgcg
     4021 gctacacctt caaattggga ggcccctcgc tgaccaatct cttgccgcgc tcataggcgt
     4081 tgcggcgcgc gccctcggct tcatcgaaca ccgccgaact gtcccgaaac gtctggctga
     4141 gctgaacggg gccgcctccg ccggttcgag tgatcgtgag gcggggacgc cacttgtcct
     4201 tttccgacgg gcgccaggcg ctggcctcga tcaggtattg cccgatttcc tcacgcaata
     4261 ccattgcatc atccgctttc aagcctaccc ccggcaaggc cgcaaagacc tcattctgcg
     4321 ccaccgcggg cgccaacgga actcaacagg aattttccgc gatcggatga atcagaggct
     4381 ttaacgacca gccgtacatc cccgaaggag gaagccatga agctcagcgc gaagaatccg
     4441 ctcccactcg cgtcccggtc tgcggccacg ggcgtgctcg tgtttgccct cgccggctgc
     4501 ggccaggaac caccgcccca accggccgcc aaggccaccc cgccggcggc accggcggcg
     4561 cccaaggccg agatcaaagc cgagccggca aagccggccg cggtcgagag cggccaggca
     4621 tccgccgaca agaccctcgc cgagcgggtg aaggccgccc tgctcgagga tcgcagcctc
     4681 aaggcgcatg gggtagatgt cgtcgtcaag gatggcatcg tcacgttgtt cgggacgacg
     4741 gaaacgcccg tccggcgcga actggccggc aggatcgcgg ccggcgtgca gggcgtcaaa
     4801 tccgtcgaga acaaacttgc ggtcgtggcc ggttcctgaa gccggcgcag ggtgaccgat
     4861 gaagcatgac ggtcgacgcc gggaacgttg acctttgtca acgaagccgc ggaaaacggc
     4921 gcggggtgcg gctccctcat tgaccccgcg cgcgcgttgc gtatagtcgc ccgctgatcc
     4981 tgctgaaacg gttcgcgctg ccggcggcat ccgcgattcg gtccggtagg gggaggtgcg
     5041 tggcatgact gtggcggcga gacatccggt acgcgtgttg ctcgtcaatc cgcgctatcc
     5101 ggaaagcttc tggagcttcc agtgggcgct cgaccgcatt ctgcggggca agcgcgccgt
     5161 caatccaccg ctcgggctcg ccacgatcgc cgcgctcacg ccgccgcact ggcgggtcga
     5221 gatcgtggac gaaaacgtgg agccggtccc ggcctggccg cgggccgaca tcgtcggcgt
     5281 gtgcgggatg gcgatacagt tcccgcgcca gaaggagctg ctcgaacgct accggcgcgc
     5341 cggttactac accgtcaccg ggggcagcta cgcctcgctg tgccccgagg cctaccacga
     5401 tatcgccgat tgcgtggtgg ccggcgaggc cgagtacgtc tggcgcgagt tctgcgcgga
     5461 ttgggagcgc ggccggctgc gcagtctcta ccgcgaaacc ggggtggtga gcctcgagga
     5521 ttctcccacg ccgcggttcg acctgctcag gctcgagcgc tacaccaccg cgacgctgca
     5581 gttctcccgc ggctgcccgt tccgctgcga tttctgcgac atcatcgtga tgttcgggag
     5641 aaagccccgc tacaagaccg tgcggcagat cgaggcggag ctcgatgccc tgcggtcgct
     5701 cggcgccggc aacgtgttct tcgtggacga caacctgatc ggccacaagg cggcctcgaa
     5761 ggagctgctc ggctttctcg ccggctacca ggcgaagcac gggcggccgt tccgcttcgg
     5821 ggcgcaggtg tcgatcaacg tggcgcacga cccggcgttc ctcaagctgc tgcgcgcggc
     5881 gaatttcacc tgggtcttca tcggcatcga gtcccccgac ctcgaggcgc tcaagcacac
     5941 gcgcaagacc cagaacaccc agcaggacct gcttacctgc gtgcggcgcg tctacgccca
     6001 cggcatcgac gtgctgggcg ggttcatcgt cggtttcgac accgacaccc cgcgcgcctt
     6061 cgacgcgcag ttccagttca tcatgcgctc gggcatccag gcggcgatgg tgggactgct
     6121 caccgcgctg ccgcgcacgc cgctctacat gcgcctggag aaagaaggac ggctgcgcgc
     6181 cggcgccgct gccagcgaca acacgaaacc cgccaccaac attatcccca agctcatgag
     6241 ctacgacgag ctggtggagg gctaccgcgc gctgcttgca aagctcacac aggaccgcca
     6301 catcgcgggg cgcatccgca acaagctcaa gtacctccac tctcccacct accagggcga
     6361 gtacggctgg ggcgagcgcc tcgcgatcct gtggaggctc ctcacgcgcg gggtgctgcc
     6421 gggcgggccg cggcgcatcc tcagcttcgc gcgcagcctg ccatggttct ccccgcgcaa
     6481 gctgccgatt gcggtcatgg actggatcgc ggggcttgcg ctgcgcgact tcctcgtgcg
     6541 ccgcttcccc gggcagtgac gcctcgctcg cgcggccgcg cagtgccggg attcatcccg
     6601 cgtcggcgta acgaaccgct ccgccttcaa t
//
LOCUS       DBNGWZ010000430         5493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_180471, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000430 DBNGWZ010000000
VERSION     DBNGWZ010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_180471"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1442
                     /gene="chrA"
                     /locus_tag="AC16CX_13005"
     CDS             99..1442
                     /gene="chrA"
                     /locus_tag="AC16CX_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR3013467.1"
                     /translation="MDKAVPSEKTPQAGVSFGDAFRVWFKIGCIGFGGPAGQIALMHR
                     ILVDERKWVDEQRFLHALNFCVLLPGPEAQKLATYIGWLLHGTRGGLAAGILFVLPGA
                     LVILGLSLLYALGRGVPAVDGALFGIKAAVLVIVVEALFRIGRRSLKTRHLIGLAGLA
                     FIAIFFLALPFPLIVAGAAVIGFLVARSSPGLLGLQAGAHGLAEPLPGRWRQAAIAVA
                     IGLGLWWAPVALAVVMLGPGHVLVDIGAFFSKLAVVSFGGAYALLAYMAQEAVESYHW
                     MSAPEMVDGLGLAETTPGPLILVTQFVGFLAAYRDASPFSPVAAGIVGSAMTTWVTFV
                     PPMLLIFAGAPFVEQLRSNQRLSGALAAITAAVVGVILNLTVWFALHVLFGSVEEMRA
                     GPLRWYAFDPAALDFKVSALALVAAVLAFVLHRGLVELVLVMAVLGIAVRLLLGT"
     gene            1593..2933
                     /locus_tag="AC16CX_13010"
     CDS             1593..2933
                     /locus_tag="AC16CX_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3013468.1"
                     /translation="MDFSRATSAPGVPPVTEKIGKYEVIKEIGKGATSAVYQAYDPFQ
                     SRLVAIKVVFPEALGDKEHGKRYRKLFVTEASLAGKLSHPHIVAIYDAVADEEANYIV
                     MEYVDGTTLEQYTRHDSLLPVQRIVEIVYKCARALEYASREGVIHRDIKPANILLAGE
                     SDIKISDFGAALTQTAETTQVSGVGSPAYMSPEQVKEQPLTYQTDIFSLGVVMYQLLT
                     GRLPFQGANNYSMIYQIINAEPPRPIQVRPDLPPRVDAIVMRALAKDTAARYQTWEEF
                     AHDLATALGREEKREQGFAESEKFDTLRKLEFFRQFTDVELWEVLRLATWHQHPRDTV
                     LIQENDIGSSFFILVSGEVKVVKQERLLNVLKRGECFGEMAYLGKQKFQRSASVVALS
                     EITVIEIRAETLSRASELCRHHFHGAFLELLVDRLAMANMRLSQLLADRSVSVF"
     gene            3298..3525
                     /locus_tag="AC16CX_13015"
     CDS             3298..3525
                     /locus_tag="AC16CX_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3013469.1"
                     /translation="MHFDKELDARGLNCPLPILRTKKALTDMASGQVLKVLATDPGSV
                     KDFQAFSKQTGNPLLSSEAIEKEFVFFMKKK"
     gene            3726..4391
                     /locus_tag="AC16CX_13020"
     CDS             3726..4391
                     /locus_tag="AC16CX_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3013470.1"
                     /translation="MTRISRRAWLARAAGGAALALEMARLRAALAAETVAKGVARVRG
                     EARIGDRPARRGMMVGPGDVVTVGRDAELVFVVGRDAFLARAGSRIELSGDAARAVVS
                     GLRLVTGALLSVFEPGGGEKRIRTATAAIGIRGTGIYVEIEAVRTYVCTCYGEAELVP
                     VDEPKEAETVRTTHHDQPRYIYPKSMPRMIEKAPVINHTDAELILLESLVGRKVPFDP
                     SLY"
     gene            4416..4778
                     /locus_tag="AC16CX_13025"
     CDS             4416..4778
                     /locus_tag="AC16CX_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013471.1"
                     /translation="MRLIEELERKLDGEQSLFRAQLLREDIARLNRLRELARTAGDLA
                     ACKKASLRLGWTQGDLRTHELRAPLEALVEAVYACEAGAGGGEQEARAIEAWTELNRV
                     RMERLVGCLATPVPKPSD"
     gene            complement(4798..5136)
                     /locus_tag="AC16CX_13030"
     CDS             complement(4798..5136)
                     /locus_tag="AC16CX_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3013472.1"
                     /translation="MKAAELQKSASRATSLLKAMSNSSRLLILCQLAEGEKSVGELER
                     SVGLSQSGLSQHLALLRRKGIVATRREAQSIYYSLASKEAEAVMATLYEVFCAKGARN
                     RPARLRETFA"
     gene            complement(5207..>5493)
                     /locus_tag="AC16CX_13035"
     CDS             complement(5207..>5493)
                     /locus_tag="AC16CX_13035"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR3013473.1"
                     /translation="KVDPGKTCATVESGKWVGPAKSAAGGEVAAVNDELVNKPSLANE
                     DPYGAGWMVIIKPENWAAVKPTLVIGSQVAPKYETKMQADGFAGCACPPK"
BASE COUNT          872 a         1794 c         1837 g          990 t
ORIGIN      
        1 ctcgtgtcac gcgggggtcg ggcagacccc ccgcttccca agacgccctg cgtccaagcg
       61 ctgcctttgc gcgacgttgc gtacgcaaag gagatgacat ggacaaagcg gtcccatccg
      121 aaaaaacgcc ccaggctgga gtgtctttcg gcgacgcatt tcgcgtctgg ttcaagatcg
      181 gctgcatcgg cttcggcggc ccggccggcc agatcgcgct gatgcaccgc atcctggtcg
      241 atgagaggaa atgggtcgac gagcagcgtt tcctgcacgc gctcaacttc tgcgtgctgc
      301 tgcccggccc ggaagcgcaa aagctggcga cctatatcgg ctggctcctg cacggcactc
      361 gcggcgggct tgccgccggc atcttgttcg tgctgccggg cgcgctcgtg attctgggcc
      421 tgagtctgct ctatgcgctc gggcgtggcg taccggcggt ggacggcgca ttgttcggca
      481 tcaaggctgc cgtgctggtg atcgtggtcg aagcgctgtt tcgcatcggc cgccgttcac
      541 tgaaaacccg tcacttgatc ggattggcag gcctggcctt catcgcgatc ttctttcttg
      601 ccctgccgtt tcccctgatc gtcgccggcg cggccgtcat cggctttctg gtcgcgcggt
      661 cgtcgcccgg cctgctcggc ctgcaggccg gcgcccacgg tctggccgag cccctgcccg
      721 ggcgctggcg acaggcggcg atcgccgtgg cgatcggcct cggcctgtgg tgggcgccgg
      781 tcgcgctcgc cgtcgtgatg ctcgggccgg gtcatgtgct ggtggacatc ggggcgttct
      841 tctccaaact cgcggtggtg agcttcggcg gcgcctatgc gctgctcgcc tacatggcgc
      901 aggaggcggt cgagagctat cactggatga gcgcgcccga aatggtggac ggcctcgggt
      961 tggcggaaac cacgcccggg ccgctcatcc tggtgacgca gttcgtgggc ttcttggccg
     1021 cgtatcgcga tgcgtcgccg ttctcgccag tcgccgccgg catcgtcggc tcagcgatga
     1081 cgacctgggt caccttcgtg ccgccgatgc tgctgatctt cgctggcgcc cccttcgtcg
     1141 agcagctgcg ctccaaccag cggctctcgg gcgcgctcgc cgccatcacc gccgcggtcg
     1201 tcggcgtcat cctcaacctc acggtctggt ttgcgctgca cgtcctgttc gggagcgtgg
     1261 aggaaatgcg cgcaggaccg ctgcgctggt acgcgttcga tcccgcggcg ctcgatttca
     1321 aggtctctgc gctcgcgctg gttgcggcgg tgctggcttt cgtgctgcac cgcggcttgg
     1381 tcgaactggt cctggtgatg gccgtgctcg ggatcgcggt gcggttgctc ctcggcacgt
     1441 gatcggcgcc gggaccagaa cctgataatt tggcagaatt cggcggccgg cgaacggaga
     1501 cggttgcccg accgcgaggg gcgtgtgacg ggcttcacgc cggcgcggtc gctccgcgcg
     1561 ttccgggtgg gggcgttatg gtatcgtgcg gcatggactt ttcgcgcgcg acctccgctc
     1621 ccggcgtgcc gcctgtaacg gaaaagatcg ggaaatacga ggtcatcaag gagatcggaa
     1681 agggcgccac cagcgccgtc taccaggcgt acgacccgtt ccagagccgc ctggtcgcga
     1741 tcaaggtggt gtttcccgag gcgctcggcg acaaggagca cggcaagcgc tatcgcaagc
     1801 tgttcgtcac ggaagcctcc ctcgccggca agctctcgca cccgcacatc gtcgcgatct
     1861 acgacgcggt ggcggacgag gaggccaact acatcgtcat ggagtacgtg gacggcacca
     1921 cgctcgagca gtacacgcgc cacgacagcc tgcttcccgt tcagagaatc gtcgagatcg
     1981 tctataagtg cgcgcgcgcg ctcgagtacg cctcccggga gggggtcatt catcgcgaca
     2041 tcaagcccgc caacatcctg ctcgccggcg agtccgacat caagatctcg gatttcggcg
     2101 cggcgctgac gcagaccgcg gagacgaccc aggtcagcgg cgtcggatcg ccggcctaca
     2161 tgtcgccgga gcaggtgaag gagcagccgc tcacctatca gaccgatatc ttctcgctcg
     2221 gcgtcgtgat gtatcaactc ctcaccggcc ggctgccgtt ccagggcgcc aacaactaca
     2281 gcatgatcta ccagatcatc aacgccgagc cgccgcggcc gatccaggtc cgtcccgatc
     2341 tcccgccgcg cgtcgacgcg atcgtgatgc gcgcgctcgc gaaggacacc gccgcgcgct
     2401 atcagacgtg ggaggagttc gcgcacgacc tcgccaccgc cctcggcagg gaggagaaac
     2461 gggagcaggg cttcgccgag agcgagaagt tcgacacgct gcgcaagctc gagttcttcc
     2521 ggcagttcac cgacgtcgag ctgtgggagg tgctgcggct cgcgacctgg caccagcacc
     2581 cgcgcgacac ggtgctgatc caggagaacg acatcggctc ctcgttcttc atcctggtct
     2641 ccggcgaggt caaggtcgtc aagcaggagc ggctgctgaa cgtcctgaag cgcggcgagt
     2701 gtttcggcga gatggcctat ctcggcaagc agaagttcca gcgctcggcg agcgtcgtgg
     2761 cgctttccga gatcaccgtg atcgagatcc gcgccgaaac gctttccagg gcctcggagc
     2821 tgtgccggca ccacttccac ggtgcgttcc tcgagctgct ggtggaccgg ctggcgatgg
     2881 ccaacatgag gctgtcccag ctgcttgccg accgcagcgt cagcgtattc tgagcgccgg
     2941 cgcgtccgct gccttgcttg cggcggcggg gaaatgaacc tatattaaga gcccctaaca
     3001 taatgaaaca cacgctgcgc tgtcacgatt ttggctgggg acatgtagca gatgcttggg
     3061 cggccggatg catgatgagt cagtgggtgt gccgtgagcc aaaatcgcgc cagcccacaa
     3121 ggggttgcgg cccaaatcgc cgctagcttt gtcgctcggc ttgccaatac agcaagtatt
     3181 ggcttcacct cgcttcgcgc tatcgacgat tttggctcgc accgcacgcg cgtttcatta
     3241 tgttaggggc tctgagttca ccgatgactt actgaattcc gcgaggcgaa gatggcgatg
     3301 catttcgaca aggaactgga cgcgcgcggg ctcaactgcc cgctgccgat actgcgcacc
     3361 aagaaggcgc tcaccgacat ggcctccggg caggtcctga aggtgctggc gaccgacccc
     3421 ggctcggtga aggatttcca ggcgttttcc aagcagaccg gcaacccgct gctctcgtcg
     3481 gaagcgatcg agaaagaatt cgtttttttc atgaagaaga agtagcgttg ttgcggacag
     3541 gggggctgcc gcaatcactc cgcgcgttcg aacggacgca gacgccgttc aacgcgcggg
     3601 gcgtgttgat gagcgcggca caggccgccg tgaacgctcc cggcgccggt ccgcccctaa
     3661 gcacggcgtt gaaacagcat ccggtgcgcc gcccgcaacg tagcatgccc ggtgaggacg
     3721 aagcgatgac gcgaatctcg cgcagagcct ggctcgcgcg cgcggcgggc ggcgcggcgc
     3781 ttgcactaga aatggcccgg ctgcgggcgg cgctggcggc ggagaccgtg gcgaagggcg
     3841 ttgcccgggt gcggggcgaa gctcgcatcg gagaccggcc cgccaggcgg gggatgatgg
     3901 tcgggcccgg cgacgtcgtc accgtggggc gcgacgcgga actcgtcttt gtcgtcggtc
     3961 gcgacgcgtt cctcgcgcgc gccggtagcc gcatcgagct ctcgggagac gccgcccggg
     4021 ccgtcgtcag cggcctgcgc ctggtgacgg gggcgctgct ttcggtgttc gagccgggcg
     4081 gcggggaaaa gcgcatccgc accgcgacgg cggcgatcgg catccgcggc accggcatct
     4141 acgtcgagat cgaggccgtc cgcacctacg tctgcacctg ctatggagag gcggagctcg
     4201 tgccggtgga cgagccgaag gaagcggaga cggtgcgcac cacccaccac gaccagccgc
     4261 gctacatcta tccgaagagc atgccgcgca tgatcgagaa ggcgcccgtg atcaaccaca
     4321 ccgacgccga gctgatcctg ctcgaaagcc tggtcggccg caaagtcccg ttcgatccct
     4381 cgctctatta gaccggggtc cgccggaaaa ccgccatgcg gctgatcgaa gagctggagc
     4441 gcaagctgga cggggagcaa agcctgtttc gcgcccagct gctgcgcgag gacatcgcac
     4501 gcttgaaccg gctgcgggag ctcgcacgaa ccgccggaga tctcgccgcg tgcaagaagg
     4561 cgtcgctgcg cctcggctgg acgcagggcg atttacgcac tcacgagctg cgtgcgccgc
     4621 tggaggcgct ggtggaagcc gtctatgcct gcgaggcggg agccggcggc ggcgaacagg
     4681 aggcgcgcgc catcgaggcc tggactgagc tcaatcgcgt ccggatggag cggctggtgg
     4741 gctgcctcgc cactcccgta ccgaaaccga gcgactgacg cgaggggtga acgcccttca
     4801 ggcgaaggtt tcccgcagcc gcgccgggcg gttcctcgcg cctttggcgc agaacacctc
     4861 gtagagggtg gccatgaccg cttcggcttc cttgctggcg agcgaataat agatggactg
     4921 cgcctcccgg cgcgtcgcca cgattccttt ccgccgcagc aacgcgaggt gctgggacag
     4981 gcccgattgg ctcaagccga ccgagcgctc cagttcgccc accgatttct ctccttccgc
     5041 gagctggcac aggatcagca ggcgcgagga attcgacatc gccttgagca gcgaggtggc
     5101 acggctggcg ctcttttgca gttcggctgc tttcactgta acgtttcctt ccgatggttg
     5161 atcgaggcgg cgcggcttgc tcgccgccga tttcatttcg gtccctttat ttcggcgggc
     5221 acgcgcatcc ggcaaatcca tccgcctgca tcttcgtctc gtatttcggc gcaacctggc
     5281 tgccgatgac gagcgtcggt ttgaccgcgg cccagttttc cggcttgatg atcaccatcc
     5341 atcccgcgcc gtagggatct tcgttcgcga gggaaggctt gttgacgagc tcatcgttca
     5401 ccgccgccac ctcgccgccg gccgccgact tggccgggcc cacccacttg ccggattcga
     5461 ccgtcgcgca ggtcttgccg ggatccacct tgc
//
LOCUS       DBNGWZ010000431         2254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_559905, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000431 DBNGWZ010000000
VERSION     DBNGWZ010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_559905"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          396 a          697 c          751 g          410 t
ORIGIN      
        1 aatttctcca gcatgggcag aggcacggcc atcctcgtcg tcgcctccga cctcgaagaa
       61 gaagcacccg tgtggtggct gcgcgtgaag caggccgccg agcgcggggc gacgctcatc
      121 gtcgcgaatg gccgcgagac gaagaccgac cgctacgccc aatacacgat ccgttaccgg
      181 tacggcgcgg aagtcgcgac ggtgctgggg atgctcgtcg ccggttcgag cgaggcggcg
      241 gacgcgtttg cgaaagccga gaacggcgtg atcctcttcg ggcgcgaggg cctcgacttc
      301 gcgggttcgt cggccctcgc ccaggcctgt gctaacctgc tcgtgaccac taaccattac
      361 ggcaagccca acaacggcct cgtggctgtg tggccgcatg cgaacacgat gggcgcgtgg
      421 gacatgggcg tgaaggcccg cgacctgtca cggccgattc cgggtgtcac cgcgtcgtgg
      481 atcgtcgccg ccgacccggt gggcgatggt ctcgtcgccg ccgaagattt gtggaaggca
      541 gggttcgtgg tcgttcagga actcttcctc accgaaaccg cgcaggccgc cgacgtagtt
      601 ctacccgcgg cggcgtgggc cgagcgcgat ggcacgttca ccaacggcga gcggcgcgtc
      661 cagcgcttct atcccgccgt gcccgccaga ggcaaacccg atttccagat cgcggcggag
      721 attggggcgc gcgtcggcgt cccattgccg aagctcgcat cacagatctt caacgaaatc
      781 gcgcaggccg ccccggccta cgccggcctg acctatcagg accttgccaa agtcgagccg
      841 cagtggccgg acatcggcgg ccgggatttc tatgggcgca actactatta cggcggcacg
      901 tcgtacgaaa attcacaggg gctgggcgtg cagctcaagt ccggcgcgga gcgcggcgag
      961 ccggtggcgg taggcaaggt ggagacgcac gaggcccggc acgatggcct cacgctcgtg
     1021 ccgaccacgc tgctctacga ccgcggcgcg acgttcgtgc gttcgctgct gatgcaccca
     1081 cgcctgccgc acccgtacgt ggaattgaac tcagccgacg cggcgaagtt gaacgtgaaa
     1141 gacggcgaca cggtgacggt ggccgccgac gggctgaagg cacaggtgac ggcccgggtg
     1201 gatggtcgcg cccccgaggg cgtggcgctg atgccggaga gcctcggccc gagcgttccg
     1261 gcgcgcgcgg tgagagcagt gatcagcaaa cagtgatcag tgaacagtca tcggtaatcg
     1321 agcgcactga ttcaccgatg actgataact gttgacagat aaccgggtga tgaaaccatg
     1381 acgactgacg aatggacgtt tcgcatcatc gaatgggtga tcaaggcggt catcaacgcg
     1441 tttgtattga tgactggctt cgcctatatg acctacgccg agcgcaaagt gtcggctctg
     1501 ttgcagacgc gccacggccc caaccgggtc ggtccgttcg gtttttttca gcccctggct
     1561 gacggcctca agctcttctt caaggaagag ctgatcccgg ggggcgcgga caagttcatc
     1621 tttctcatcg cgcccatcat cacggtcgtg cccgcgctgg tgatcctcgc cgtggtgccg
     1681 tgggggccga aggtgtacat cctcgggcgc gaggtggagc tcggagtcaa ggatctcaat
     1741 gtagggatgc tctacattct ctcggtggcc tcgattgcgg tgtacggcgt ggtgctggga
     1801 ggctggtcgt cgaacaataa gtatgcgatg ctcggcggca tccgctccac ggcgcagatg
     1861 gtgagctacg aactcgcgct gggactgtcc accatcggcc cgctcatgct tgccggctcg
     1921 atgagcatga acgacatcgt cgttgcgcag aagggcgtgt ggtacgtctt ctaccagccg
     1981 gtcgccgccc tcatcttcta cgtcgccgcg ctggccgagg tcaaccgcgc gccgttcgac
     2041 atgcccgagg ccgagcagga gttgacggcc gggtatcaca ccgaatactc cgggatgaag
     2101 ttcgcgctgt tcttcatggg cgagtatatc aagatgatcg gcgtgagcgc gatcttcgtc
     2161 acgctgttct tcggcggctg gcaaggcccg ttcgtcgagc aaatcccgtg gctcggcctc
     2221 ctgtacttct ggctcaaggt cgccggatcg ctgc
//
LOCUS       DBNGWZ010000432        15512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_560382, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000432 DBNGWZ010000000
VERSION     DBNGWZ010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_560382"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1041)
                     /locus_tag="AC16CX_13050"
     CDS             complement(1..1041)
                     /locus_tag="AC16CX_13050"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HIR3013474.1"
                     /translation="MHIDRDIKLDFKDVLIRPKRSALPSRAEVDVHREFVFKHSQRKY
                     RGIPIIAANMDTVGTFEMARALAAFDLSVALHKHYGVDELVRYFKSLKVKSSAFYSMG
                     ITRDDHEKFKRVVQRAGEAIEYVCIDVANGYTEGFVKFVEKFRQDHPKITVMAGNVVT
                     GEMTEELILCGVDIVKVGIGPGSVCTTRSITGVGYPQLSAVIECADAAHGLGGRICAD
                     GGCAAPGDIAKAFGGGADFVMLGGMLAGHKECAGKIVGKGGKQYKRFYGMSSRTAMEK
                     YAGGVAHYRAAEGREILVEYHGPVAVTVREILGGVRSACTYVGARQLRELTKRTTFVR
                     VRGEADEVFDES"
     gene            1325..2245
                     /locus_tag="AC16CX_13055"
     CDS             1325..2245
                     /locus_tag="AC16CX_13055"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013475.1"
                     /translation="MAVILIENFRAVFYAPFYAAAALGAYEAEGVEVDMKMSAAADRT
                     LQTLAAGAGQVSWGGPLRIMTALDRDPKGGYVAFCEVVGRDPFFLVGRTPNPGFALGQ
                     LPRWTLATVTEVPTPWICLQHDLRRAGFDPSSVRRAPGRTMAENAAALRAGEAELIQV
                     FQPYARILTDEGAGHVWYAAASRGPTSYTTFNTTRSFIERNPDTVLRMTRAMYRTLKW
                     IAARDGRDLAEAIARYFPDVPVAVLAACCNDYRSLGLWNRTPVVQREGLEWLRDAMLA
                     AGAIRSRFAYEDCVETRFAEQVVSEDPPAF"
     gene            2366..3229
                     /locus_tag="AC16CX_13060"
     CDS             2366..3229
                     /locus_tag="AC16CX_13060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013476.1"
                     /translation="MTMAYNPAAQYEVKVWDVEFRRAPTRTLMARVYQPQGAGPFPTL
                     LDLHGGAWNDKDRLANAPMDQSLAASGVLVVAIDMTLASEAPYPASVQDANYGIRWLK
                     AKAREWKGDPATVGALGSSSGGHVIELCGMRPRDPRYNALHLPGASGMDAAPAYIATR
                     SPISDPYARFQQAQKMKRDEMIQKTRTYFDPWETIYEGNPQQMLDRGEAVSLPPLLIM
                     QGELDDNVLPALQEKFAASYRAAGGECQLEVFPGCEHMWVAQPGPQTERANEMVKAFI
                     ARQLRALRRAA"
     gene            3294..4130
                     /locus_tag="AC16CX_13065"
     CDS             3294..4130
                     /locus_tag="AC16CX_13065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3013477.1"
                     /translation="MSGVNTLYRVTLHHVTFHHVTLHHVTPYLIQEKRTMPSKNIRIR
                     SSEGGEFDCYLATPDAGGKAPAIVLASAVHGVDRDIRSIADEFAAHGFIAAAPDLFWR
                     SIPGPLSRDDDRTKQRSQPRLEKIKAGEADMADTLAEVRKLPQFNGRAAAMGFCYGGP
                     YAILGPKRLGYDAGISCHGTQMLDFFRELDGVAQPVCIIWGDQDHAAPPAVQNAYRGV
                     PARMKNVEVHIFPGVLHGYMMRGNAKAFSQETRDFSMGRALAILDGLRGEGRRQSLRK
                     AV"
     gene            complement(4287..5249)
                     /locus_tag="AC16CX_13070"
     CDS             complement(4287..5249)
                     /locus_tag="AC16CX_13070"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013478.1"
                     /translation="MSDKFRAAAALLAALACGDVHAQAYPQRPIRMVIGLPPGGSTDI
                     VGRVVAAKLSERIGQQVVVDNRPGASGIIGITLVVNSLPDGYTLIMAAGSWGTISSLY
                     KLPFDLQRDLAPVALIGTSPYLLVVQPTLPVKTVADLIAYAKANPGKLNYAGSTPGSL
                     QRLAGELLKRTAGIDMLYVPYKGTGAVMPDLLAGRLHAAFDNVLVLTPYVRTGQLRAL
                     GVTSTKRSVIFPELPTIAETGVPGFHAVGWFGVFTAAKTPQRIVTRLNAEISAFMKEP
                     DMRERLLKQGAEPLSGPPEDLRNYLAGEIEKWGRVIREAGIKAE"
     gene            5369..6343
                     /locus_tag="AC16CX_13075"
     CDS             5369..6343
                     /locus_tag="AC16CX_13075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013479.1"
                     /translation="MRLTLGISHAVAIAAACALGMPAAAQSWPARPVRIIVPYSPGGG
                     IDTVARVLAQKLTEQTGGSFVVENRPGGAGVLGAELVARAAPDGHTLLASATEFAINP
                     GVRSKLPYDPFKDFTHISQLASVQFILGSHPSVPVRNVKQLVALAKARPGQLTYGSSG
                     TGAGPHLAGELLQSMAGIRWVHVPFKGAAPAAVAVISGEIDFVFGATIGLLGNVQAGK
                     LRAVAVTGLKRFMELPQVPTVAESGVPGYSATGWYGFYAPAGTSPELVRRLHAEATRA
                     LGAADVKEKLARSGNEYVMSPPEEFVAFLRAEIVKWTKVVREANIRIE"
     gene            complement(6431..7393)
                     /locus_tag="AC16CX_13080"
     CDS             complement(6431..7393)
                     /locus_tag="AC16CX_13080"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013480.1"
                     /translation="MTGRWLLAALASLACGAAAAQAYPAKPVRIIVGFTPGGGNDAIS
                     RLVANKLTAAFGRQFIVENRPGAGGMIGAELAAKSPPDGYTLFLAGVATHAINPNLQK
                     NVPYDPVRDFDPVCLMASAPTLLAVHPSLPARSVKQFIALARARPGEINFASSGHGSS
                     AQLATELFKSMTGTRMQHVPYKGIGSALSDLLSGQVQMMFNAAAALLPHTRSGRLRAL
                     GVGALQRVAAIADIPTIAESGVPGYQAGSWYGFVAPAGTPRAIIDRLSRETAAAIKSP
                     EISSKLTAEAVLVIGSSPEEFAAHLKDELARIGKVIREAGITME"
     gene            complement(7422..8387)
                     /locus_tag="AC16CX_13085"
     CDS             complement(7422..8387)
                     /locus_tag="AC16CX_13085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013481.1"
                     /translation="MRQFVGSLVAGVVVAMASVGVSAQAFPTRPVRLVVPFPTGGATD
                     TFSRATAAEMSKSLGQQVIVENRPGAGTTIAAELVAKAAPDGHTLFFTDLSTHAITAS
                     LYTKLSYHPLRDFAAVAPVSASPLLMVAHPSVGVKSARELIALAKKRPGITCGNAGIG
                     TVTHMAAEKFRMRAGIELTPVNYKGGATSTISLLTGEIALVVTTIPAAIEYVRVRRLV
                     ALGVTAERRSALLPDIPALGETVKGVDAAVIAGVLAPAVTPRAVIERLNAEFAKAVDS
                     PKVKEIFAVNAADAMKASPAELQQALERDVKVWAEVVKATGVPLQ"
     gene            complement(8689..9075)
                     /locus_tag="AC16CX_13090"
     CDS             complement(8689..9075)
                     /locus_tag="AC16CX_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3013482.1"
                     /translation="MDKQLMQAGLAARRAVLGDEYVDRAMKSADSFNQPFQELVSEYC
                     WGVCWTDDKLTRRERSILNLGMIAALGKMHEFELHFRGALRNGLTEDELRSVLIQIAV
                     YCGIPVGVDCFRTARQVLADVKGGGK"
     gene            complement(9253..10605)
                     /gene="ffh"
                     /locus_tag="AC16CX_13095"
     CDS             complement(9253..10605)
                     /gene="ffh"
                     /locus_tag="AC16CX_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3013483.1"
                     /translation="MFDSLSGRLSRVIKTLRGEARLTESNIQDALREVRVALLEADVA
                     LPVVRVFIERVRERALGEEVAGSLTPGQAAVGVVYRELVRLMGEKNDSLDLATRPPAV
                     ILLAGLQGSGKTTTAGKLARWLKSELGKKVLLASCDVYRPAAIEQLATLARQVEVEFF
                     PSAAGEKPLDIATRALDYARKHYADVLIVDSAGRLAIDEAMMREIGELHAALKPIETL
                     FVVDAMQGQDAVNTAKAFADALPLSGVILTKLDGDARGGAALSVRQVTGKPIKFAGVG
                     EKLAALEPFHPDRMASRILGMGDVLSLIEDVSKSVDREEAEKLAKKVKSGKDFDLEDF
                     RQQVAQMKKMGGLAALADKLPGDLGRLAQGAPGMDEKTIRRIEGIINAMTPQERAKPG
                     ILKASRKRRIAAGAGVTVQEVNRLLNQFEQTQKMMKMMAKGGIAKMMRNMKGMFPGMR
                     "
     gene            10648..11478
                     /locus_tag="AC16CX_13100"
     CDS             10648..11478
                     /locus_tag="AC16CX_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR3013484.1"
                     /translation="MPEILPYLANALLYAALAFYFWRTRWSPAGNAQAAEASFNVFEH
                     YAVLVPLGLHTVLLARAAFAQDGLHLGVGNAVSAILWLTVLIYWLGNFHYRLDGLQSL
                     VLPVAAAASLLPAVFPSLKPLPNTDLMVFKVHLLIAMLAYSLFTIASLHVLLMALLER
                     RLHDGSLPRALRGLPPLLAMEALLFRIIWAGFILLTLTLATGVVYSEELFGRAAKFNH
                     KTVFGVLSWIIFAALLGGRHVYGWRGRVAVRWTLTGFLMLVLAYVGSKFVLEIILGRA
                     "
     gene            11478..12749
                     /locus_tag="AC16CX_13105"
     CDS             11478..12749
                     /locus_tag="AC16CX_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR3013485.1"
                     /translation="MEDIPLWAELATLFVLLLISAYFSLAETAMMALNRYRLNHLVAQ
                     GHGGARLAADLLKRTDKLLGAILIGNTLVIAAATTITSLVAVRLLGEDKVVYAISTVV
                     ISFLIIVFSEITPKVVGATYPERTALPLAYVLGPTMWLLEPAAWFVNLFVRPLLALLR
                     IRPEEHTEAPKLTPEEIRTLVLESSSFMPKKHVSILLNLFDLEAITVDDVMVPRNHIE
                     AVDLSAPLEEIRQQIATAHHRRVLVYRGYLDEVVGTLRTRDVLNLVQNEELTKEKLGE
                     IIREPYFVPAGTPLFSQIQSFQEQQDRMSLVVDEYGELMGLVTLEDILEEMVGEFTTH
                     SPLQTGGFTRQPDGSYLVEGATPLRELNRKLGFCFPLDGPKTLNGLILEHLQDIPEPN
                     TSIKIAGHPLEIVQTQGRAVKAVRMFPATSA"
     gene            12871..14100
                     /locus_tag="AC16CX_13110"
     CDS             12871..14100
                     /locus_tag="AC16CX_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3013486.1"
                     /translation="MATAAAAVRKPDTKEFNFSWVGTDKANKTVRGEMKATGEAQVNA
                     TLRRQGIRVVEIKKQKLGRGGSITDKDITLFVRQLATMMKSGVPLLQAFDIVGRGHSN
                     PAVARLLLEIKTEVETGSSLRQAFEKHPLYFDALFCNLVGAGEQAGILDSLLDRLATY
                     KEKILAIKSKIKSALFYPVAIVIVAFIITAVIMIFVIPAFKQVFSSFGADLPAPTLIV
                     MSISEFFVTYWYAIFGGIGGAGYAFFYTWKRSKAMQIFMDRVMLRLPVFGNLVRISCI
                     ARWCRTLSTMFAAGVPLVEALESVAGASGNYEYYAATKKIQGEVSTGSSLTSSMQGTD
                     VFPSMVIQMVQIGEEAGSLDAMLSKVADFFEAEVDDAVEALSSLMEPMIMVVLGTLIG
                     GLVVAMYLPIFKLGGAV"
     gene            14336..15187
                     /locus_tag="AC16CX_13115"
     CDS             14336..15187
                     /locus_tag="AC16CX_13115"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3013487.1"
                     /translation="MLSALQQPAVLVPMCIFFGLCVGSFLNVVIHRLPQMLERQWRAE
                     CAELTGSAPPPATKFNLLAPGSACPKCGYRITAFENIPIASYIALGGKCSKCKAPIPL
                     RYPVVEALAGLLAGYAAWRYGLSAALLGALVFAWAMVALAFIDLEKLYLPDSITLPLL
                     WAGLLLNLGATFTDLRSAVIGAAAGYLALWAVFWAFKLATGKEGMGYGDFKLLAAIGA
                     WLGWKMLPLTILLSSFIGAAVGIGLIVFARHGRDKPIPFGPYLALAGLIALFWGEGIN
                     RRYLAFF"
     gene            15189..>15512
                     /gene="coaE"
                     /locus_tag="AC16CX_13120"
     CDS             15189..>15512
                     /gene="coaE"
                     /locus_tag="AC16CX_13120"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668272.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3013488.1"
                     /translation="MPYCVGLTGGIGSGKSTAAKMFEALGVAVVDTDALSHELTAAGG
                     AAMAAISERFGPEYVAADGSLDRERMRRFVFRDAAAREKLEAILHPMIRAAARARGAA
                     ARAPYV"
BASE COUNT         2515 a         5258 c         4993 g         2746 t
ORIGIN      
        1 ctagctttcg tcgaagactt cgtccgcctc cccccgcacg cgcacgaacg tggtccgctt
       61 ggtcagttcc ctcagctgcc gcgcgccgac gtaagtgcag gcggagcgca cgccgcccag
      121 gatctccctc acggtgacgg cgaccggtcc atgatattcc accaggattt ccctcccctc
      181 cgccgcgcgg tagtgcgcga cgccgccggc gtacttctcc atcgcggtgc gcgagctcat
      241 cccgtagaag cgcttgtact gcttgccgcc cttcccgacg atcttgccgg cgcattcctt
      301 gtgcccggcc agcatgcccc ccagcatcac gaagtccgcc ccgccgccga atgccttggc
      361 gatgtcgccg ggggcggcgc agcctccgtc ggcgcagatc cggccgccga ggccgtgcgc
      421 ggcgtccgcg cactcgatca cggccgagag ttgaggatag cccacgccgg ttatgctgcg
      481 cgtggtgcag accgagccgg ggccgatgcc gaccttgacg atatcgacgc cgcacaggat
      541 cagctcctcg gtcatttcgc cggtcacgac attgccggcc atgacggtga tcttcgggtg
      601 gtcctggcgg aacttctcga cgaacttgac gaagccttcg gtatagccgt tggcgacgtc
      661 tatgcacacg tactcgatcg cctcgccggc ccgctgcacc acgcgcttga acttctcatg
      721 gtcatcccgg gtgatgccca tcgaatagaa cgcgctcgat ttcaccttga gcgatttgaa
      781 atacctgacg agctcgtcca ccccgtagtg cttgtgcaac gccaccgaca ggtcaaacgc
      841 cgccagcgct cgcgccatct cgaacgtgcc gacggtgtcc atgttcgcgg cgatgatggg
      901 gatgccgcga tacttgcgct gcgagtgctt gaagacgaac tcgcggtgaa catcgacttc
      961 cgcgcgcgag ggcagcgccg agcgcttggg gcggatcagc acgtccttga agtcgagctt
     1021 gatgtcccgg tcgatgtgca tggcatcctg aacgatgacg ccgcagcctg ccgccgagtg
     1081 tcaacgaagg gcaaccgtga catgtttcat cggcctgcgg cctggcggga acccaaacga
     1141 ggacaacata acacgggatt tgcgattttc ttaccgggct ctgcagcgcg acccgtgaag
     1201 tagcggcaat ctactgatcc aaaaagtatt tatgtgattc gcgcgatttc acttgctcat
     1261 acgccgatgt cccgatgctt tatattgacg cctccgaccg actccgtcgg aaacagcaca
     1321 agcaatggcc gtcatcctga tcgagaactt ccgcgcggtg ttctacgcgc cgttctacgc
     1381 ggccgccgcg ctcggcgcgt acgaggcgga gggcgtggag gtggacatga agatgtcggc
     1441 ggcggcggac cgcaccctgc aaacgctcgc cgccggggcg gggcaggtat cgtggggcgg
     1501 gccgctgcgg atcatgaccg ccctcgaccg ggatccgaaa ggcggatatg tcgcgttctg
     1561 cgaggtggtg gggcgcgatc cgttctttct ggtcgggcgc accccgaacc ccgggtttgc
     1621 gctcgggcag ctcccgcgat ggacgctcgc gacggtcacc gaggtgccga cgccgtggat
     1681 ctgcctccag cacgatctgc gccgggccgg gttcgacccc tcctcggtcc ggcgcgcgcc
     1741 cgggcgcacc atggcggaga atgccgccgc tttgcgcgcg ggcgaggcgg agctgatcca
     1801 ggtgttccag ccttacgcga ggatcctgac cgacgaaggc gcagggcacg tttggtacgc
     1861 cgccgcgagc cgcggaccga cgtcgtacac cacgttcaac acgacgcgca gtttcatcga
     1921 gcggaacccc gacaccgtgc tgcgcatgac gcgggccatg taccggacgc tcaagtggat
     1981 cgcggcgcgt gacgggcgcg acctcgctga ggccatcgcc cgctattttc cggacgtgcc
     2041 ggttgcggtg ctcgccgcct gctgcaacga ttaccggtcg ctcgggctgt ggaaccgcac
     2101 gcccgtcgtg caacgcgagg gcctcgagtg gctgcgcgac gccatgctcg cggcgggagc
     2161 gatccggagc cggttcgcct acgaagactg cgtggaaacg cgtttcgccg agcaggtcgt
     2221 gagtgaagat ccgcccgcgt tttgattctt cgcgatccag cgtcgggcaa cgaacgggaa
     2281 aaagcggggt ccgcccggtc tgttttgggg taagatttcc ttctttcgaa tatcggctct
     2341 gccggaacgg aagggggatc gcaagatgac gatggcttac aatcccgcgg ctcaatacga
     2401 ggtcaaggtg tgggacgtcg agttccggcg cgcgccgacg cgcacgctga tggcccgcgt
     2461 ctatcaaccg cagggcgcgg gaccgttccc gaccctgctc gacttgcacg gcggcgcctg
     2521 gaacgacaag gaccgcctgg ccaacgcgcc gatggaccag agcctggcgg ccagcggcgt
     2581 gctggtggtg gccatcgaca tgacactggc ctcggaggcg ccatatccgg cttcggtcca
     2641 ggatgctaac tacggcatcc gctggctcaa ggcgaaagcg cgcgaatgga aaggcgaccc
     2701 cgccacggtc ggcgcgctcg gcagctccag cggcggtcac gtgatcgagc tctgcggcat
     2761 gcgtccccgc gatccgcgct ataacgcgct gcacctgccc ggggcttcgg gcatggacgc
     2821 ggcgccggcc tacatcgcga cccgttcgcc gatcagcgac ccgtacgccc gcttccagca
     2881 ggcgcagaag atgaagcgcg acgagatgat ccagaaaacg cggacttact ttgacccgtg
     2941 ggagacgata tacgaaggca atccgcagca gatgctcgac cgcggcgagg cggtttcact
     3001 gccgccgctg ctgatcatgc agggcgagct cgacgacaac gtgctgccgg cgctgcagga
     3061 gaaattcgcc gcctcttatc gggcggccgg cggcgagtgc cagctggagg tttttcccgg
     3121 ttgcgagcac atgtgggtcg cgcagccggg gccgcaaacc gagcgcgcca acgagatggt
     3181 caaggctttc atcgcccgcc agctgagggc gttgcgccgc gcggcgtgat atccctgata
     3241 cttcgtgaat ggtgaatggt gaatagtgaa tggtaagcgg tgagcagtga gcagtgagcg
     3301 gtgtaaacac gctttaccgc gtcacgctcc atcacgtcac gttccatcac gtcacgctgc
     3361 atcatgtcac gccttatctg attcaggaga aaagaaccat gcccagcaag aacatcagga
     3421 tccgctcgag cgagggcggc gagttcgatt gctacctggc gacgcccgac gccggaggca
     3481 aggcgccggc catcgtgctc gcctccgccg tgcatggcgt cgaccgggac atacgctcga
     3541 tcgcggacga gttcgctgcg cacggcttca tcgcggcggc gcccgatttg ttctggcgct
     3601 cgattccggg tccgctgtcg cgcgacgacg accgcaccaa gcagcgttcg cagccgcgcc
     3661 tcgagaaaat caaggcgggc gaagccgaca tggcggacac cctggccgag gtgcgcaaac
     3721 tgccgcaatt caacgggcgc gccgcggcga tgggtttctg ctacggcggc ccctatgcga
     3781 tcctcgggcc gaagcgcctc ggctatgacg ccggtatctc ctgccatggc acgcagatgc
     3841 tggacttctt cagggagctc gacggggtcg cccagcccgt ctgcatcatc tggggcgacc
     3901 aggaccacgc ggcgcccccc gcggtgcaga acgcctaccg cggcgtgccg gcgcgcatga
     3961 aaaacgtgga agtgcacatc ttcccgggcg tgctccatgg ttacatgatg cgaggcaacg
     4021 cgaaggcgtt cagccaggag acacgcgact tctccatggg gcgcgcgttg gcgatcctcg
     4081 acgggcttcg cggcgagggc cggcggcagt cgctgcgcaa ggcggtttga gtcggcccaa
     4141 gcagaagggc gtgacgggtg ataagtgata ggactttaac ggcgtaattc ctgacaagtg
     4201 ataaaaaaag gtcggcgacg ccggccgcga cacttatcac ttatcaccta tcacgttttt
     4261 tccctcatcc ccccgtccct cgggggtcac tcggccttga tgcccgcctc gcgtatcacc
     4321 ctgccccact tttcgatttc gcccgcgaga tagttgcgca gatcctcggg cgggccggac
     4381 agcggctccg cgccctgctt gagcaggcgc tcgcgcatgt ccggctcctt catgaacgcc
     4441 gagatttcag cattgagcct ggtcacgatg cgttgcgggg tcttcgccgc cgtgaaaacg
     4501 ccgaaccagc ccaccgcgtg gaagcccggc acgccggttt ccgcgatcgt cggcagctcc
     4561 ggaaaaatga ccgagcgctt cgtgctggtc acccccaggg cgcgcagctg gccggtccgg
     4621 acatagggcg tgagcaccag cacgttgtcg aatgccgcgt gcagccggcc ggccagcagg
     4681 tccggcatca ccgcgccggt gcccttgtac ggcacgtaga gcatgtcgat gcccgccgtg
     4741 cgcttgagca gctcgccggc caggcgctgt aacgatcccg gcgtggaccc ggcgtagttc
     4801 agtttgcccg ggttcgcctt ggcgtaggca atcaggtccg caaccgtctt caccggcagc
     4861 gtcggctgga ccaccagcag gtacggcgag gtcccgatga gcgcgaccgg cgccaggtcc
     4921 ctctggaggt cgaacggcag cttgtagaga ctgctgatcg tgccccacga gcccgccgcc
     4981 atgatcagcg tgtaaccgtc cggcagcgaa ttgaccacga gcgtgatgcc gatgatgccg
     5041 ctcgcgcccg gccggttgtc caccacgacc tgctggccga tacgctcgga gagcttggcc
     5101 gcgacaacgc ggcccacgat gtcggtgctg ccgccgggcg gaagccctat gaccatgcgg
     5161 atcgggcgct gcggataagc ctgcgcgtgc acatcgccgc aggcaagcgc cgcgagcagc
     5221 gcggctgcgg cgcggaattt atcgctcatg gcctcttccc gtcgctttct ccgtcactcg
     5281 acacccgaca cccaacactc atcactcgga ttctcgattc aggctaatat gcgcgctcgc
     5341 gtgccgtcaa caaccgacag ggggagacat gaggctcact ctaggcattt ctcatgcggt
     5401 cgcgatcgcc gccgcgtgcg cgctcggaat gcccgccgcg gcgcagagct ggcccgcccg
     5461 cccggtgcgg atcatcgttc cctacagccc ggggggcggg atcgacaccg tggcgcgcgt
     5521 gctggcgcag aagctgaccg agcagaccgg cggctcgttc gtggttgaaa accgtcccgg
     5581 cggggccggc gtcctcggcg ccgagctcgt cgcgcgggcg gcgccggacg gccacacgtt
     5641 gctggcgtcg gcgacggaat tcgccatcaa ccccggcgtc cgctcgaagc tgccgtacga
     5701 cccgttcaag gatttcacgc acatctcgca actcgcctcc gtgcagttca tattggggag
     5761 ccacccctcg gtgccggtga gaaacgtcaa gcaactcgtc gcgctggcga aggcccgccc
     5821 cggccagctc acctatggct cctccggcac cggcgccggt ccgcacctgg ccggcgagct
     5881 gctgcagtcg atggcgggta tccgctgggt gcacgtgccg ttcaagggag cggcgcccgc
     5941 ggctgtcgcg gtcatcagcg gcgagatcga cttcgtgttc ggcgccacca tcggactgct
     6001 cggcaacgtg caggccggca agctgcgcgc agtcgccgtc accggcttga agcgcttcat
     6061 ggagctgccg caagtgccga ccgtcgccga atcgggcgta ccggggtaca gcgcaacggg
     6121 ctggtacggc ttctatgctc ccgccggcac ttctccggaa ctcgtgcgcc ggcttcacgc
     6181 cgaggcgacg cgggcgctcg gcgccgccga cgtcaaggag aagctggcgc gcagcggcaa
     6241 cgagtacgtc atgagcccgc ccgaggaatt cgtggctttt ctccgcgccg agatcgtcaa
     6301 gtggacgaag gtggtcaggg aagcgaatat acgcatcgag taaaaccgcc ctgaactgcg
     6361 gtgaggcgtg ccgtgtgagg aggagctttc tgcggacgcc ggtttcatcc ctaatccccc
     6421 ggatgcctcg tcactccatg gtaatgccgg cttcccggat caccttgccg atgcgcgcaa
     6481 gctcgtcctt gaggtgagcg gcgaattcct cgggtgagct gccgatcact agcacggcct
     6541 cggcggtcag cttcgaggaa atctccggcg acttgatcgc ggcggcggtt tcccggctca
     6601 gccggtcgat gatcgcccgc ggcgttccag ccggcgcaac gaacccgtac cacgatcccg
     6661 cctggtagcc gggcacgccc gactcggcga tggtcggaat gtcggcgatc gcggcgacgc
     6721 gctgcagcgc gccgacgccg agcgcgcgca gccggcccga ccgggtgtgg ggcagcagcg
     6781 cggcggcggc gttgaacatc atctgcacct gtccgctcag caggtcgctc aacgcggagc
     6841 cgatgccctt gtacggcacg tgctgcatcc gggtgcccgt catcgacttg aacagctcgg
     6901 tggcgagctg ggccgagctg ccgtggccgc tcgaagcgaa attgatctcg ccgggccgcg
     6961 ccctggcgag cgcgatgaac tgcttcaccg agcgcgccgg cagcgacgga tgcaccgcca
     7021 gcagcgtcgg tgcgctcgcc atcaggcaca ccgggtcgaa atcccgcacc gggtcgtacg
     7081 ggacgttctt ctgcaggtta gggttgatcg cgtgggtcgc gaccccggcg aggaacagcg
     7141 tgtagccgtc gggcggcgat ttcgccgcca gctcggcgcc gatcattcca cccgcccccg
     7201 ggcggttctc gacgatgaac tgccggccga aggcggcggt cagcttgttc gcgaccaggc
     7261 gcgaaatggc gtcgttgccg ccgccgggcg tgaacccgac gatgatgcgg accggcttcg
     7321 ccgggtaggc ctgcgccgcg gccgcgccgc aggcgagcga tgcgagcgcg gcaagcaacc
     7381 atctccctgt cattttcgca cccgataaag tgtgtaaccc gttactgcaa tgggacgccg
     7441 gtcgccttca ccacctccgc ccacactttc acgtcccgtt ccagcgcctg ctgcagctcg
     7501 gcggggctcg ctttcatggc gtccgcggca ttgacagcga agatctcttt caccttgggg
     7561 ctgtccacgg ccttcgcgaa ctccgcattg agccgctcga tcacggcgcg cggcgtgacg
     7621 gcgggcgcca gcaccccggc gatcacggcg gcatccacgc ctttcacggt ctcgccgagg
     7681 gcggggatgt cgggcagcag cgccgaacgt cgctccgcgg tcacgcccag cgccaccagc
     7741 ctgcgcacgc gcacgtattc gatcgccgcc gggatggtgg tgacgacgag ggcgatctcc
     7801 ccggtgagga gggagatcgt cgaggtggcc ccgcccttgt agttgaccgg cgtcaactcg
     7861 atgcccgcgc gcatccggaa cttctcggcc gccatatggg tgacggtgcc gatgcccgcg
     7921 ttgccgcagg tgatccccgg ccgcttcttc gctagcgcga tcagctcgcg ggccgacttc
     7981 acgccgaccg aggggtgcgc gaccatcagc agtggggagg cgctcaccgg cgcgaccgcg
     8041 gcgaaatccc gcagcggatg gtacgagagc ttcgtgtaga gcgaggccgt gatggcgtgc
     8101 gtcgagaggt cggtgaagaa cagcgtgtgg ccgtcgggcg cggccttggc gacgagctcg
     8161 gcggcgatcg tggtgcccgc cccgggtcgg ttttcaacga tcacctgctg ccccaggctc
     8221 ttgctcatct ccgccgccgt cgcgcgggag aacgtgtcgg tcgccccgcc agtcggaaac
     8281 ggcaccacca gccgcaccgg ccgggtcgga aacgcctgcg cggaaacgcc gaccgacgcc
     8341 atcgcgacga caaccccagc aaccagactg ccgacgaatt gtctcatcgc cttctcctcc
     8401 ctaagagtcc ctaacataat gaaacacacg ctgcgctgtc acgattttgg ctcgaaacgt
     8461 tcagcatgtg cgggaaaagc atattgaagc aaggccgatg gttgtgcctc aggccaaaat
     8521 cgcgccagcc cacaaggggt tgcggcccaa atcgccgcta gctttgtcgc tcggcttgcc
     8581 aatacagcga gtattggctt cacctcgctt cgcgctatcg acgatttcgg ctcgcaccgc
     8641 acgcgcgttt catcatgtta gggactctaa atgatctcca accggtgtct atttcccgcc
     8701 acccttcaca tcggccagca cctgccgcgc cgtgcggaag caatccacgc cgaccggtat
     8761 gccgcaatac accgcgatct ggatcagcac cgagcgcagc tcgtcttccg tgagcccgtt
     8821 gcgcagcgcg ccgcgaaaat gcagctcgaa ctcgtgcatc ttcccgagcg ccgcgatcat
     8881 gcccaggttg aggatgctgc gctcgcgccg cgtgagcttg tcgtcggtcc agcacacgcc
     8941 ccagcagtac tcgctgacca gttcctggaa cggctggttg aagctgtcgg cgctcttcat
     9001 cgcccgatcg acgtactcgt cacccagcac cgcacggcgc gcggccaggc ctgcttgcat
     9061 cagctgcttg tccataggtt ctcctttcga gggtcaatgc aacaaaatgg ccgcggcgtc
     9121 ggatccggaa tttccgcagc ccctgctccg cgccccgcac cagtcaagct ggcgccttgt
     9181 gtccggtttc aaccctgccc gggcgcgtaa aacaaagtcg ccggtccttc agcgctctga
     9241 acgggatgct ggctaccgca tccccgggaa catgcccttc atgttccgca tcatcttggc
     9301 gatgccgccc ttcgccatca tcttcatcat tttctgcgtc tgctcgaact ggttgagcag
     9361 ccggttcact tcctggaccg tgacgccggc gccggccgcg atgcggcgct tgcgcgaggc
     9421 cttgaggatc ccgggcttcg cccgctcctg cggcgtcatc gcattgatga tgccctcgat
     9481 gcggcggatc gtcttctcgt ccatgcccgg cgcgccctgc gcgagccgcc cgaggtcccc
     9541 cgggagcttg tccgcgagcg cggcgagccc gcccatcttc ttcatctgcg cgacctgctg
     9601 cctgaaatcc tcgaggtcga agtcctttcc cgacttcacc ttcttcgcga gtttctccgc
     9661 ctcctcgcgg tccacgctct tgctcacgtc ctcgatcagc gagagcacgt cgcccatgcc
     9721 gaggatgcgc gaggccatgc ggtccgggtg gaacggctcc agggcggcga gcttctcgcc
     9781 gacgccggcg aacttgatcg gcttgccggt cacctgccgc accgagagcg ccgcgccgcc
     9841 gcgggcgtcg ccgtcgagct tggtgaggat gacgccgctc aacggcagcg cgtcggcgaa
     9901 cgccttggcc gtgttgaccg cgtcctggcc ctgcatcgcg tccaccacga acagcgtctc
     9961 gatcggcttc aacgccgcgt ggagctcgcc gatctcgcgc atcatcgcct cgtcgatggc
    10021 gagccgcccc gcgctgtcca cgatcagcac gtccgcatag tgcttgcgcg cgtaatccag
    10081 ggcgcgcgtc gcgatgtcga gcggtttctc gccggcagcg gacgggaaga actcgacctc
    10141 aacctgccgc gcgagcgtgg cgagctgctc gatcgccgcc gggcgataga cgtcgcagct
    10201 cgcgagcagc accttcttcc cgagctcgct cttcagccag cgcgcgagct tgcccgccgt
    10261 ggtggtcttg cccgagccct gcagtcccgc cagcaggatc accgcgggcg ggcgggtcgc
    10321 gagatcgagc gagtcgttct tctcgcccat gaggcgaacg agctcgcggt agaccacgcc
    10381 caccgccgcc tgccccggcg tgaggctgcc ggccacctcc tcgcccagcg cccgctcgcg
    10441 cacgcgctcg atgaagacgc gcaccaccgg cagcgcgacg tcggcttcca gcagcgccac
    10501 gcgcacttcg cgcagcgcat cctggatgtt ggactcggtg aggcgcgcct cgccgcgcag
    10561 cgtcttgatc acgcgcgaca ggcgcccgct caggctgtcg aacatggact tatcatcact
    10621 ccgccggggt tggtgtagac tcgaaacatg ccggaaattc taccttacct cgctaacgcg
    10681 ctgctctacg cggcgctcgc gttctatttc tggcgcacgc gctggagccc ggcgggcaac
    10741 gcgcaggccg ccgaggcttc cttcaacgtc ttcgagcatt acgcggtgct cgtgccgctc
    10801 ggcctgcaca cggtgctgct ggcgcgcgcc gcattcgcgc aggacgggct gcatctcggc
    10861 gtcggcaacg cggtctcggc gatcctgtgg ctcacggtgc tgatctactg gctcggcaat
    10921 ttccattacc ggctcgacgg cctgcagtcg ctggtgctgc cggtggcggc ggcggcctcg
    10981 ctcctgccgg cggtattccc ctcgctcaag ccgctgccca acaccgatct catggtgttc
    11041 aaggtccatt tgctgatcgc gatgctcgcc tacagcctgt tcaccatcgc ctccctgcac
    11101 gtgctgctga tggcgctcct cgaacggcgc cttcacgacg gcagcctgcc gcgggcgctg
    11161 cgcgggctgc cgccgttgct cgcgatggag gcgctgttgt tccgcatcat ctgggcggga
    11221 ttcatcctgc tgacgctcac gctcgcgacc ggcgtggtct actcggagga gctgttcgga
    11281 cgcgcggcga agttcaacca caagacggtg ttcggcgtgc tgtcgtggat catattcgcg
    11341 gcccttctcg gcggccggca cgtctatggc tggcgcgggc gcgtcgccgt gcgctggacg
    11401 ctcaccggct ttctcatgct ggtgctcgcc tatgtcggca gcaagttcgt gctcgaaatc
    11461 atcctcgggc gcgcctgatg gaagacatcc cgctctgggc ggaactcgcc acgctctttg
    11521 tgctgctcct catttcggcg tacttttcgc tcgccgagac ggcgatgatg gcgctcaacc
    11581 gctaccggct caaccatctt gtcgcccaag gccacggcgg cgcccggctc gcggcggacc
    11641 tgctcaaacg caccgacaag ctgctcggcg cgatcctgat cggcaacacc ctcgtgatcg
    11701 cggccgcgac cacgatcacg agtctggtcg cggtgcggct gcttggggag gacaaggtgg
    11761 tgtacgcgat cagcaccgtc gtgatctcgt ttctcatcat cgtcttttcc gagatcacgc
    11821 cgaaggtggt cggcgccacc tatccggaac gcaccgcgct gccgctcgct tacgtgctcg
    11881 ggccgaccat gtggctgctc gagcccgcgg cgtggttcgt gaaccttttc gtgcggcctc
    11941 tgctcgctct gttgcgcatc aggccggagg agcataccga ggcgccgaag ctcacgcccg
    12001 aggaaatccg cacgctcgtg ctcgagtcct cgagcttcat gccgaaaaag cacgtctcga
    12061 tactgctgaa cctgttcgac ctcgaggcga tcacggtgga cgacgtgatg gtgccgcgca
    12121 atcacatcga agcggtcgat ctctccgcgc cgctcgagga aatccgccag cagatcgcca
    12181 ccgctcatca ccggcgggtg ctggtctacc ggggctatct ggacgaggtg gtgggcacgc
    12241 tgcggacgcg cgatgtgctc aacctggtgc aaaacgagga actgaccaag gagaagctcg
    12301 gggaaatcat ccgcgagccg tacttcgtgc cggccggcac gccgctgttc tcccagattc
    12361 agagcttcca ggaacagcag gaccggatga gcctggtggt ggacgagtac ggcgagctga
    12421 tggggctggt gacgctggaa gacatcctcg aggagatggt cggcgagttc accacgcact
    12481 cccccctgca gaccggcggc ttcaccaggc agcccgacgg cagctatctc gtggagggcg
    12541 ctacgccgct gagggagctc aaccgcaagc tcggcttctg ttttccgctc gacgggccga
    12601 agacgctgaa cgggctcatc ctcgagcacc tgcaggacat tcccgagccc aataccagca
    12661 tcaagatcgc cggccacccg ctcgaaatcg tccagaccca ggggcgggcg gtgaaagcgg
    12721 tgcggatgtt tcccgcgacg agcgcctgag tgcaccgcgg cccgcggcga ctgtggcaaa
    12781 ctaggcttta caaagtcagc gaagatgggc atcattttac ggataagcga agctaacctc
    12841 ttggttcatt agggtaatcg ggggatcacc atggcaactg cggccgcagc agtcagaaaa
    12901 ccggatacca aggaattcaa tttctcctgg gtcggcacgg acaaagccaa caagaccgtg
    12961 cgtggcgaga tgaaggcgac cggcgaagcc caggtcaacg ccaccctgcg ccgccagggc
    13021 atccgggtgg tcgagatcaa gaagcagaaa ctgggccgcg gcggctcgat caccgacaag
    13081 gacatcacgc tgttcgtgcg gcagctcgcg accatgatga aatcgggcgt gccgctgctg
    13141 caggctttcg acatcgtcgg caggggtcac agcaatccgg ccgtcgcgcg gctgctgctg
    13201 gagatcaaga ccgaggtcga gaccggttcg agcctgaggc aggccttcga gaagcacccg
    13261 ctctatttcg acgcgctgtt ctgcaacctg gtgggcgcgg gcgagcaagc cggtattctg
    13321 gattcgctgc tcgaccggct cgcgacctac aaggaaaaga tcctggcgat caagagcaag
    13381 atcaagtccg ccctgttcta tccggtggcc atcgtcatcg tggccttcat catcacggcg
    13441 gtgatcatga tcttcgtgat cccggcgttc aagcaggtgt tctcgagctt cggcgccgac
    13501 ctgccggcgc cgacgctgat cgtgatgtcg atatccgaat tcttcgtgac atactggtac
    13561 gccatcttcg gcggcatcgg cggcgcgggt tatgccttct tctacacctg gaagcgctcc
    13621 aaggcgatgc agatcttcat ggaccgcgtg atgctgcggc tgccggtgtt cggcaacttg
    13681 gtcagaatat cgtgcatcgc gcgctggtgt cgcacgctct ccaccatgtt cgctgccggc
    13741 gtaccgctgg tcgaggcgct cgagtcggtg gccggcgctt ccggcaacta cgaatactat
    13801 gccgcgacca agaagatcca gggcgaggtc tcgaccggat cgagcctgac ctcctcgatg
    13861 cagggcaccg atgtcttccc gagcatggtg atccagatgg tgcagatcgg cgaggaggcc
    13921 ggctcgctcg acgccatgct gtccaaggtc gccgacttct tcgaggccga agtggacgac
    13981 gcggtcgagg cgctctcgag cctgatggag cccatgatca tggtggtgct cggcacgctc
    14041 atcggcggcc tggtggtcgc gatgtacctg cccatcttca agctgggcgg cgcggtctag
    14101 aaaccggtga ggagtcaggg gagaggtgtg aggcataaac cacccctgac caccgaaccc
    14161 cgccacccgc cacccacctc ccgacttccg cctcacccgg cgatgatgct cgtcgaggca
    14221 tttcgccgat cgcctgcgct cctcgtcacc ctcgcggtgc tgcccggcct gatggtcggc
    14281 ggctttggtt ccggctcaat atgcgcaatc gcatgttaac ctccactgga accgtatgct
    14341 gagcgcactg cagcagccgg cggttttggt gccgatgtgc atcttcttcg gcctgtgcgt
    14401 cggcagtttt ctgaacgtcg tcatccaccg cctgccgcag atgctggagc ggcagtggcg
    14461 cgccgagtgc gccgagctga ccgggagcgc gcccccgccg gcgacgaagt tcaatctgct
    14521 cgcgccgggc tcggcctgcc cgaagtgcgg gtaccggatc accgcgttcg agaacatccc
    14581 gatcgcgagc tacatcgccc tcggtggaaa atgctcgaaa tgcaaggcgc ccatcccgct
    14641 gcgttacccg gtcgtggagg cgctcgccgg gctgctcgcc ggctacgccg cctggcgcta
    14701 tggcctgagc gccgccctgc tcggtgcgct cgtcttcgcc tgggcgatgg tcgcgctcgc
    14761 cttcatcgat ctcgagaagc tctacctgcc ggacagcatc acgctgccgc tgctctgggc
    14821 cggattgctg ctcaatctcg gcgccacttt caccgacctg cgctcggccg tgatcggcgc
    14881 cgcggccggc tatctcgcgc tgtgggccgt gttctgggcc ttcaagctcg ccaccggcaa
    14941 ggaaggcatg ggctatggcg acttcaagct gctcgccgcg atcggcgcct ggctcggctg
    15001 gaaaatgctg ccgctcacga tcctgctctc ttccttcatc ggcgcggccg tgggcatcgg
    15061 gctgatcgtc ttcgcgcgcc acgggcgcga caaaccgatc ccgttcggcc cctacctggc
    15121 actcgcaggg ttgatcgcgc tgttctgggg cgagggaatc aaccggcgct atctcgcctt
    15181 tttctagcat gccctactgc gtcggcctca ccggcggcat cgggtcgggc aaatcgaccg
    15241 ccgcgaagat gttcgaggcg ctcggcgtgg cagtggtgga caccgacgcg ctttcgcacg
    15301 agctcactgc cgcgggtggc gcggcgatgg ccgcgatcag cgagcggttc gggccggagt
    15361 acgtcgccgc ggacggcagt ctcgaccgcg agcggatgcg gcgcttcgtg ttccgggacg
    15421 cggcggccag ggagaaactc gaggcgatcc tgcacccgat gatccgcgcc gccgcgcgcg
    15481 cgcgcggcgc cgccgcgcgc gcgccctacg tc
//
LOCUS       DBNGWZ010000433         3979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3327772, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000433 DBNGWZ010000000
VERSION     DBNGWZ010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3327772"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16CX_13125"
     CDS             complement(<1..404)
                     /locus_tag="AC16CX_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3013489.1"
                     /translation="MKKLQERLVDTVVATIIAVACFTNAAAQTVIDEWESAKFPPPPA
                     LKPATLNPAETALLVMDFTNQTCSSARRPRCAASVPKVQKLVAEARARGALVIYSVAV
                     AGSTAADILKDLTPAAGELVLPPLGPDKFINS"
     gene            775..1143
                     /locus_tag="AC16CX_13130"
     CDS             775..1143
                     /locus_tag="AC16CX_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3013490.1"
                     /translation="MTLKLNHLHLKTKDPEKTAKFYVDNLGATVVSKSPNGGYRLDLH
                     GLHLNVSDFLVNQKREQKYGMEHIAIETDELDALVEKLKAQGIHILEQTVVSGGRRVC
                     FFEGPDGVQLEFIEMKGSGK"
     gene            1286..2392
                     /locus_tag="AC16CX_13135"
     CDS             1286..2392
                     /locus_tag="AC16CX_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3013491.1"
                     /translation="MAPSNPLRVGLIGAGGRWGPWAHVPALQGLPETELYAVCTAHAD
                     TAQAAADKYGVKHAYSDVEALGKDPQVEAALVAVRVPAHYALTKTALEAGKHVYCEWP
                     LGANTKEAEELAAMARKKKLQTVVGLQRRASPAYLYMRELIRDGYVGEMLSVNMMLMN
                     SGVLTRTSDRTWQRDAKLGANPFTITFGHSIDALCMVVGELIEVSAIVSTRVPQWFET
                     DTKKYVDTTAPDNIMIQGRLENGAVVSAYVGVQPYLGSGYRFEIYGKDGTLMMLGGGE
                     AGQEANRRIFGGHKEDKALQELPVPERLKWVPEAVRKVGRAYDVGQMWVNFSKAIRTG
                     KNVEPDFDHAVRRHRMLDAIMRASETGQRQKVAL"
     gene            2487..3593
                     /locus_tag="AC16CX_13140"
     CDS             2487..3593
                     /locus_tag="AC16CX_13140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013492.1"
                     /translation="MALKRPKGPSGTLVVLEHASKILKDNPLGDPHVRKLGVWLPPQY
                     DERGGRRRFPVLFDLVGFTGSGLSHTNWKPFGDNVPERAARLIRERKMGPAIFVFPDC
                     FTALGGNQYVNSSAIGDYADYLTREIVPFVDREFRTLAGREHRGCFGKSSGGYGAIIH
                     GMRYAKYWGAIADHSGDAYFDFVYWHDWPNTLNELAKHRLPKRRAGAYDARRESSRKG
                     LAEGLDDGRIKRFLAHVWKKEKLSTAEGHCIMNLCMAATYDPDPGVPPGFRVPFNLES
                     GEVIEKRWRRWREHDPINLVTRHRDNLKVLRGIYIDCGWRDQYHIHYGTRILSKRLAL
                     AGVRHRYEEFDDNHSDIDYRMDVSLPFLYRALKP"
     gene            3644..>3979
                     /locus_tag="AC16CX_13145"
     CDS             3644..>3979
                     /locus_tag="AC16CX_13145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3013493.1"
                     /translation="MLRIYGSAKSRTLRVLWMAGELGLSYDHKDYLPRSPETRTPEYR
                     TLNPNGRVPTIDDDGFVLSESMAINFYLAKKHKSPLYPTDPGHEALVLQWSLWETDRL
                     DRQIVNYCRH"
BASE COUNT          771 a         1271 c         1276 g          661 t
ORIGIN      
        1 tcgctgttga tgaatttgtc cggcccgagc ggcggcagca cgagctctcc ggccgcgggg
       61 gtgaggtctt tcaggatgtc ggcggcggtg gagcccgcga ccgcgacgct gtagatcacg
      121 agcgcgcctc gcgcccgcgc ctcggcgacg agcttctgga ccttgggcac cgaggctgcg
      181 cagcgcgggc gccgtgccga gctgcaggtc tggttggtga aatccatcac cagcagcgcc
      241 gtctccgccg gattgagggt tgctggtttc agagcgggcg gcggcggaaa cttcgcgctt
      301 tcccactcgt cgatgacggt ctgtgccgcc gcgttcgtga aacaggcaac cgcaatgatc
      361 gtggcgacga ccgtatcgac cagccgctcc tggagctttt tcatgtttcc cccttccgag
      421 aaagatgagt cgtgggtgaa atgcgggcac actcgacttt gctcggaagt atcgcgccaa
      481 accccggccg gcgccacagg ggacgaagcc cccttgtggc gggtgatgcg tgatgggtga
      541 tgagtgatgg agcggaagga tgacccgagg gactccctgg ataccatcct tggcgttgtc
      601 ttagcggcct ctcaagagcc atgactggtg actcgtgact gggctccgcg cggtgcgcgc
      661 aagactcatc actcatcacc catcacgcat caccgatatt gggcgtcctt tgcagtgagg
      721 ctatttttac gttagctttg gctacttgat ttgccacgcc ggaggaggtg tctcatgacg
      781 ctgaagctca accacctgca tctgaagacc aaggacccgg agaagacggc gaagttctac
      841 gttgacaacc tcggcgccac ggtcgtgagc aagtccccga acggcggcta ccgcctggat
      901 ctgcacgggc tgcacctcaa tgtctcggac ttcctcgtga accagaagcg cgagcagaag
      961 tacggcatgg agcacatcgc catcgaaacc gacgagctgg acgcgctcgt ggaaaagctc
     1021 aaggcccagg gcatccacat cctcgagcag accgttgttt caggcggccg ccgggtgtgc
     1081 ttcttcgagg ggccggatgg cgtgcagctc gagttcatcg agatgaaggg gagtggcaag
     1141 tgaggaggta tctccaggga tgaaggcaga aggcagaagg atgacctagc cagtatcgtc
     1201 ttcccgcact gcggggatca tcccccatcc ctcatccttc gaggcggccc atcacccatc
     1261 actcgtaagt aaaggagcag gaacaatggc gccatcaaat ccattgcgcg tggggctgat
     1321 cggagcgggc gggcgctggg gcccatgggc gcatgtcccg gcccttcaag ggctgccgga
     1381 gaccgagctt tacgccgtct gcacggcaca cgcggacacg gcgcaggcag cggccgacaa
     1441 gtacggcgtg aagcacgcgt acagcgacgt cgaggcgctg ggcaaggacc cgcaggtcga
     1501 ggcggccctc gtcgccgtgc gcgttccggc gcactatgca ctcacgaaga ccgcgctcga
     1561 ggcgggcaag cacgtctact gcgagtggcc cctgggcgcg aacacgaagg aggcagagga
     1621 gcttgccgcg atggcgcgca agaagaagct gcagacggtg gtcggcctgc agcggcgcgc
     1681 gtcacccgcg tacctctaca tgcgcgagct gatccgggac ggctacgtcg gggaaatgct
     1741 gtcggtgaac atgatgctga tgaacagcgg cgtgctgacc cgcacctcgg accgcacctg
     1801 gcagcgcgac gcgaagctcg gcgcgaaccc gttcaccatc acgttcggac actcgatcga
     1861 tgccctgtgc atggtcgtgg gtgagctcat cgaggtgtcg gctatcgtca gcacgcgggt
     1921 gccgcagtgg ttcgagaccg atacgaagaa gtacgtggac accaccgcgc ccgacaacat
     1981 catgatccag ggccggctcg aaaacggcgc agtggtcagc gcgtacgtgg gcgtccagcc
     2041 ctaccttggc agcggctacc ggttcgagat ctacggcaag gacggcacgc tgatgatgct
     2101 gggcggcggg gaagcggggc aggaggcgaa ccgcaggatc ttcggcggcc ataaggagga
     2161 caaggcgctg caggaactgc ccgtgccgga gcggctcaag tgggtgccgg aggcggtacg
     2221 gaaagtgggc cgcgcctacg acgtgggcca gatgtgggtc aatttctcca aggcgatccg
     2281 caccggcaaa aacgtcgagc ccgacttcga ccacgcggtg cgccgccacc ggatgcttga
     2341 cgccatcatg cgcgcttcgg agacggggca acggcagaaa gtcgcgctgt aagccggtga
     2401 caactaatga ctgacgactg accgggcccc cgcgcggagc gggggaaact catcacccat
     2461 tacccatcac tcatcaccga tttcagatgg ctctcaagcg cccgaaaggt ccttccggca
     2521 cgctggtcgt gctggagcac gcttcgaaga ttctcaagga caacccgctc ggcgatccgc
     2581 acgtgcgcaa gctcggcgtg tggctgccgc cgcagtatga cgaacgcggg ggcaggcgcc
     2641 gtttccctgt gctcttcgac ctggtcggct tcaccggctc cggtctttcg cataccaact
     2701 ggaagccgtt cggcgacaac gtcccggagc gcgcggcgcg gctcatccgc gagcggaaga
     2761 tggggccggc gatctttgtc tttccggact gcttcacggc gctcggcggc aaccagtacg
     2821 tgaactcgtc cgccatcggc gattacgcgg actacctcac gcgcgagatc gttcccttcg
     2881 tggaccgcga attccgcacg ctcgctggcc gcgagcaccg cggctgcttc ggcaagtcat
     2941 ccggcggcta cggcgcgatc attcacggca tgaggtacgc gaaatactgg ggcgccatcg
     3001 ccgatcattc cggcgacgcg tatttcgatt tcgtctactg gcacgactgg ccgaacacgc
     3061 tcaacgagct cgcgaagcac cgcctgccga agcgaagagc cggcgcgtac gacgcgcgcc
     3121 gcgagtcaag ccgcaagggc ctcgccgaag gcctggacga cgggcgcatc aagcgcttcc
     3181 tcgcgcacgt ctggaaaaag gaaaagctct cgaccgcaga aggccattgc atcatgaacc
     3241 tctgcatggc cgccacctac gacccggatc cgggggttcc gcccggcttt cgcgtcccgt
     3301 tcaacctcga atcgggcgag gtgatcgaga agcgctggcg ccggtggcgc gagcacgacc
     3361 cgatcaacct cgtcacaaga caccgggaca acctcaaggt gctgcgcggc atctacatcg
     3421 attgcggctg gcgcgaccag tatcacatcc attacggcac gcgcatcctc tccaagcggc
     3481 tcgctctggc cggcgtgcgg caccgctacg aggaattcga cgacaaccac tccgacatcg
     3541 attaccggat ggacgtgagc ttgccgtttc tctacagggc gttgaagccc tgaacgccaa
     3601 gcctcgtgta gactcccgtt tccacccggc tccggaggac atcatgctgc gcatctacgg
     3661 ctccgccaaa tcccgcacgc tccgcgtact gtggatggcg ggcgaactcg gactttccta
     3721 cgatcacaaa gactacctgc cgcgctcgcc ggaaaccagg acgccggaat accgcacgct
     3781 caatcccaac ggccgcgtgc cgacgatcga cgacgacggg ttcgtgctct cggagtcgat
     3841 ggcgatcaac ttctatctcg cgaagaagca caagagcccc ctctacccca ccgaccccgg
     3901 gcacgaagcg ctggtgctcc agtggagcct gtgggagacc gaccgcctcg accgccagat
     3961 cgtaaactac tgcaggcac
//
LOCUS       DBNGWZ010000434         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2070487, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000434 DBNGWZ010000000
VERSION     DBNGWZ010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2070487"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1739)
                     /locus_tag="AC16CX_13150"
     CDS             complement(87..1739)
                     /locus_tag="AC16CX_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR3013494.1"
                     /translation="MARGGAQQGQGSIHPLRAVACAASGRCLVAVLALVFVAGGALGA
                     CSRAPIGTEAIAPTTVVPQRDWSDAIIYFALIDRFADGDSANNAGVDGRNPGAYHGGD
                     LTGLLQQLDEIASLGATALWVNPVVKQIDKPVWAQGPPGSGWERGFEHWGFHGYWADD
                     FERLEPHFGGEAELRALVEAAHARGLKVLLDVVYNHPGYGAHYLTDPATRNWLRVPDG
                     DCGADPLTCQVGGLPDFRTELPEVREYLFRAHLGLARRVGLDGFRLDTVKHVEHDFWQ
                     AHRARSRAELGPDFFLLGEVWGGSYEVLDPWFANGEMDAGFDFSFRGSCQGFVEGKGR
                     TVAFAAYLKKRHQVRHGYHLAQYLSSHDEPMLLHELGDDPQKFKLCVALQMTSSGIPV
                     IYYGEEVGRRGGAWPTNRGDMPWGERAVAPGHGIPRDEALRTYYRHLIALRRTHPALT
                     RGTFRELSTAGDLLVFAREDEASGDAVIVAINRGTDAAAAAVPPPERWRGLAVREALS
                     GEPAMLEDGRLAIKAPPRTALVYITEAGTRGAIVWPTFASRT"
     gene            1969..>2431
                     /locus_tag="AC16CX_13155"
     CDS             1969..>2431
                     /locus_tag="AC16CX_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013495.1"
                     /translation="MCKVRGVAVSSKLCPKIILEGTRLTLKTEIAFALNEHSRIVGPR
                     KYRYPSPLISAEWCAFTNVPWGRGLVNFDPSEEALAIETYRTWVRLFELLRYYSWIVD
                     RFHISTQAYQLQAHGKKYDFGWLEERLLPLGFRIVFCTRAPGSFEAARRERL"
BASE COUNT          413 a          846 c          779 g          393 t
ORIGIN      
        1 cgacggcccg accagcacga tgaattcctc gtcggcgatc tcgaggctga ggtcctcgat
       61 cacgctcacc gcgccgaaac gcttggctac gttcttgaag cgaatgtcgg ccatacgatc
      121 gcgcccctcg ttccagcctc cgtgatataa accagcgccg tgcgcggcgg tgccttgatc
      181 gccagccgcc cgtcctccag catcgccggc tcgccactca gcgcttcacg caccgccagc
      241 ccgcgccagc gctccggcgg cggcaccgct gccgccgcgg cgtccgtgcc gcggttgatg
      301 gcgacgatga cggcgtcgcc agaggcctca tcctctcgcg cgaacaccag caggtcgccg
      361 gcggtcgaca gctcgcggaa tgtcccgcgt gtgagcgccg ggtgtgtccg gcgcagtgcg
      421 atcagatgcc ggtagtaggt gcgcagcgcc tcgtcgcgcg gtataccgtg gccgggcgcc
      481 acggcgcgct cgccccacgg catgtcgccg cggttggtcg gccacgcgcc cccgcgcctt
      541 cccacctcct cgccgtaata aatcaccgga atgccggagc tggtcatctg cagcgccacg
      601 cacagcttga acttttgcgg gtcgtcgccg agctcgtgca acaacatcgg ctcgtcgtgc
      661 gacgacaggt actgcgcgag gtgatagccg tgccgcacct gatggcgttt cttgagatag
      721 gccgcgaagg caacggtgcg ccctttaccc tcgacgaacc cctggcagct gccccggaag
      781 ctgaagtcga agccggcgtc catctcgccg ttggcgaacc acgggtcaag cacctcgtac
      841 gagccgcccc agacctcgcc aagcaggaag aagtccggtc ccagctcggc gcggctgcgc
      901 gcgcggtgcg cctgccagaa gtcgtgctcg acgtgcttga cggtgtcgag gcgaaaacca
      961 tcgaggccga ctcgccgcgc gagaccgaga tgggcgcgaa acagatactc ccgtacctcg
     1021 ggcagttcgg tccggaaatc cggcaggcca ccaacctggc aggtgagcgg atcggcgcca
     1081 cagtcaccgt cgggcacgcg cagccagttg cgggtggcgg gatcggtgag gtaatgcgcc
     1141 ccgtaaccgg gatggttata gaccacgtcg agcagcacct tgagaccgcg cgcgtgtgcg
     1201 gcctcgacca gcgccctgag ctcggcctcg ccgccgaaat gcggctcgag gcgctcgaag
     1261 tcgtcggccc agtagccgtg aaagccccag tgctcgaagc cacgttccca tccgctgccc
     1321 ggcggcccct gcgcccacac cggtttgtcg atctgcttga ccaccggatt gacccagagg
     1381 gcggtagcgc cgagtgaggc gatctcgtcg agctgctgca aaaggcccgt gaggtcgccg
     1441 ccgtgatagg cacccgggtt gcgcccgtcg acgcccgcgt tattcgccga gtcaccgtcg
     1501 gcgaagcgat cgatcagcgc gaagtagatg atggcatcgg accagtcgcg ctgcgggacg
     1561 accgtagtcg gggcgatcgc ttccgtgccg atcggggcac gggaacaggc ccccagtgcg
     1621 ccacctgcaa cgaacaccag cgcaagcacc gccaccaggc aacggccgga cgcagcgcaa
     1681 gccacggcac gcagcgggtg gatggagccc tgtccttgtt gtgctccgcc tcgtgccatc
     1741 gctccctatg gccccgaaca tgcgaatgga tcgagcctat gcggcgacac gaccgacaga
     1801 ccctggtggt gccccgtatc ctgaccgccc gtgattgtgc gtcagcggcg cttctcgggt
     1861 caacgccggc acccgcgaca aacgtacaga cgagttatac tgcgatcctg ccgtgccatc
     1921 ggcgtttgcg cgcggtgcgc cgtataccga gacgcgagcg gatcggacat gtgcaaagtg
     1981 agaggcgtcg ctgtgtcgtc aaaactctgc cccaagatca tcctcgaggg gactcggctt
     2041 accctcaaga ccgagatagc ctttgcactc aacgagcatt cgcgcattgt cggcccgcgc
     2101 aagtaccgct acccctcgcc gctgatctcc gccgaatggt gcgcgttcac caacgtccca
     2161 tggggacgcg ggcttgtcaa cttcgacccg agcgaggagg cgctcgccat cgagacctac
     2221 cgcacctggg tgcggctgtt cgaactgcta cgctactact cgtggattgt ggaccgcttt
     2281 cacatctcga cccaggccta ccaactgcag gcccacggca agaagtacga ctttggctgg
     2341 ttggaggagc gcctgctgcc gctcgggttc cgcatcgtgt tctgtacgcg agcgccgggg
     2401 tcgttcgagg cggcgcggcg cgagcggctc a
//
LOCUS       DBNGWZ010000435        12733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3203730, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000435 DBNGWZ010000000
VERSION     DBNGWZ010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3203730"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1714)
                     /locus_tag="AC16CX_13160"
     CDS             complement(38..1714)
                     /locus_tag="AC16CX_13160"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3013496.1"
                     /translation="MPLSDIEIAQRAKMLRITQVAGKLGIAEEHLVPYGHYKAKVSLD
                     FVDSLNNRKDGKLVLVTAITPTPAGEGKTTTTVGLCDALNRIGKKTVLCIREPSLGPV
                     FGIKGGAAGGGYAQVVPMEDINLHFTGDFGAISLANNLLAAAIDNHIHHGNELGIDVR
                     RITWRRVMDMNDRALRDITVGLGGVGNGYPRQDGFDIVPASEVMAIFCLATSLKDLKN
                     RLGNIVVGYTRDQKPVRARDLKVDGAMTVLLKDALAPNLVQTLENNPAFIHGGPFANI
                     AHGCNSVIATRTALRLADYCVTEAGFGADLGAEKFIDIKCRKAGLRPDAVVIVATIRA
                     LKYHGGVNIKEVNKENLAALEKGIANLERHVNNIRNHYGLPCVVSINRFNFDTPAEIE
                     LLKKKMAHHEAPVILATHWADGGKGAEEVARTVVNLIENVPTDFKFVYDDALPLWDKI
                     KTIATKIYGAADVAAEGKVRTQIKKLQDDGYGHYPVCVAKTQYSFSADASLRGAPSGY
                     MVNVREVRLAAGAEFIVMICGDIMTMPGLPKVPSAEKIDLADDGKVVGLF"
     gene            complement(1877..4687)
                     /gene="fdhF"
                     /locus_tag="AC16CX_13165"
     CDS             complement(1877..4687)
                     /gene="fdhF"
                     /locus_tag="AC16CX_13165"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR3013497.1"
                     /translation="MTAMSKEEQAAMPVEFKLNGSEVVGDANETLIQTAKRYGIEIPH
                     LCYKEGMRPDGNCRACMVEIKGERVLTPSCCRKPSKGMEVFTDNARSLASQKMVLELL
                     LSDIPKRRHTLNSELDRWAAKLKVGKPRFAPRHQPKPDLSHYGIAVNLDHCIQCTRCV
                     RACREEQVNDVIGYAFRGEHSKIVFDLDDPMGESTCVACGECVQACPTGALMPARGVG
                     MVKPDKQVHSVCPYCGVGCQLTYAVKDNRILYVDGRDGPSNHGRLCVKGRYGFDYVHH
                     KQRLTKPLVRKPGVPKHADFTMDPANPLEYFREATWEEALDLAAGRLKEIRDQNGKHA
                     LAGFGSAKGTNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAALMEGISSGAVSNQV
                     NDVARAEVIFLIGANPVSNHPVAATWIKNAAKQGAKLIYADPRRSDLSRHAAYYLQFK
                     PDTDVALLNAMMHTIVHEGLVDEDFIKGRTIGFEELQKNVAGYSPEAMAPVCGIPAGT
                     IREVARLYAKSRASMILWGMGISQHVHGTDNARCLIALCLMTGQIGRPGTGLHPLRGQ
                     NNVQGASDSGLIPMVFPDYQRVDNNDARSRFEKLWGTTLDPKPGLTVVEIMHAVLDGK
                     IRGMYVMGENPAMSDPDAHHAREALARLDWLVVQEIFLTETCYYADVILPATAWPEKD
                     GTVTNTDRMVQLGRRALDAPGEARPDLWIIQEIARRIGLDWNYGGAADVFNEMRKAMP
                     SIAGITWQRLEAESCVTYPCEKEGDPGQPVVFTEKFPTPTGRAKFVPADIIPANERPD
                     RHYPFVLITGRQLEHWHTGAITRRAAVLDAIEPEPTASLHPLDIEALGAKPGDVITVE
                     SRRGIISLYARADDGTPRGAVFIPFCFYEAAANLLTNPALDPFGKIPEFKYCAVKVMR
                     GGELMPRSSYGGGRALAELERV"
     gene            complement(4784..6481)
                     /locus_tag="AC16CX_13170"
     CDS             complement(4784..6481)
                     /locus_tag="AC16CX_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR3013498.1"
                     /translation="MQGGTLEGGIVKKRRDGDARGKPKGRLVDPRALAEVQALLGDGP
                     RRRDLLVEHLHKIQDRYGQLSAAHLVALAREMNLAPAEVYEVATFYHHFDVVKKGETA
                     PPPLTVRVCDSLSCDLAGAHELIGELKRALGPSVRMIPAPCVGRCEQAPVAVVGQNPV
                     AQATVGKVKPLAAAGKTQCPVSRYITYAEYRKKGGYRIAAECLAGKRDAESIMKTMED
                     SGLRGLGGAGFPAGRKWRIVRAEQAPRLLAINLDEGEPGTFKDRYYLERDPHRFLEGA
                     IIAAWTVQIAQVYIYLRDEYAGCREILERELKALQADPPCALPPLFLRRGAGAYICGE
                     ESAMIESIEGKRGMPRLRPPYVAQVGLFGHPTLEHNMETLYWVREILEKGAAWFASQG
                     RHGRKGLRSFSVSGRVKKPGVHVAPAGITVKELIEEYCGGMLPGHAFYAYLPGGASGG
                     ILPASMGDIPLDFDTLNQYGCFIGSAAVIILSDRDQARAAAANVMKFFADESCGQCTP
                     CRVGTAKALTLMQAPRWDQGLLEELSGAMADASICGLGQAAPNPIRCVVKYFPHEIK"
     gene            complement(6520..7398)
                     /gene="sucD"
                     /locus_tag="AC16CX_13175"
     CDS             complement(6520..7398)
                     /gene="sucD"
                     /locus_tag="AC16CX_13175"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3013499.1"
                     /translation="MSILINRNTRVMTQGITGKTGQFHTKMGKEYANGARCYVAGVTP
                     KKGGQSFEGIPIFETVREAKEKTGATVSVIYVPPPFAAAAIDEAVEAGLELVICITEG
                     IPVRDMIRTRYKMQGSKTLLVGPNCPGVITPDEIKIGIMPGHIHRKGPVGVVSRSGTL
                     TYEAVGQLMEAGLGQSTCVGIGGDPVNGLKHVDVLEMFNDDPQTEAVVMVGEIGGTNE
                     EEAARWVRANMKKPIVGFIAGVTAPPGKRMGHAGAIISGGKGTAAEKLAVMEECGIKI
                     TRNPAEMGRLLKSVLK"
     gene            complement(7485..8651)
                     /gene="sucC"
                     /locus_tag="AC16CX_13180"
     CDS             complement(7485..8651)
                     /gene="sucC"
                     /locus_tag="AC16CX_13180"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3013500.1"
                     /translation="MKIHEYQAKEILRKYGIATPRGYPCFSVEEAVEAAKKLGGSVWV
                     VKAQIHAGGRGKGGGVKLTKSLEEVQRHAAQILGMQLKTHQTGPQGQKVRRLLIEEGA
                     DIRKELYLGMVVDRGSQRVVLMASSEGGMDIEEVAAKSPEKIHKVTIDPAMGLADRDA
                     EEIARKIGVPQPAVPQARDFLKGLYRAFDETDASLAEINPLIVTGDERVLALDAKMNF
                     DDNGLFRHPEIVAMRDLDEEDPAEVEASKFDLSYISLDGNIGCLVNGAGLAMATMDII
                     KLYGGSPANFLDVGGGASTEKVTEAFKIMLHNPGLKAILVNIFGGIMKCDVIAEGVVT
                     AARQVSLKVPLVVRLEGTNVELGKKILAGSGLPIISANNMADAAQKVVAAARGK"
     gene            complement(9245..9685)
                     /locus_tag="AC16CX_13185"
     CDS             complement(9245..9685)
                     /locus_tag="AC16CX_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3013501.1"
                     /translation="MFKNILIPTDGSELSQRAVRTAVGLAKLHQARITGIHVIPDYHL
                     LIAYEGAFDPVTEERIEEEAKTRAEGYLAFVRKTAEDAGVPCDTLCETSDHPYEAILK
                     SAEARDCDLIVMTSHGRKGLAAVLLGSETRKVLTHAKIPVLIVR"
     gene            9855..10892
                     /locus_tag="AC16CX_13190"
     CDS             9855..10892
                     /locus_tag="AC16CX_13190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3013502.1"
                     /translation="MRHQLLLTGDVNLMKVGDPRVPFARVAETLRTADLVFGNLECCL
                     YSPPAERSLADEGFYAPPAAGEALRLAGYHVVGTANNVNYGFEAITHSLRELDRLGIA
                     HAGSGANRVAAHAPAVIERNGLRFGLLQRTSVYWPTDHEAGEHSAGVAVIRGHTAYQV
                     PAHKTRPEIPPMNRPGVPPVILTWADADYLARFREDLTALRGRADIVVASHHWGLHQE
                     VLQYMTEVAHMAIDAGADLVVGHGPHFVLPVEVYKGKPIFYGMGNFSFHTGHGGRKHG
                     DWVGLLARVELESRAIRRVALTFVRHNDRNETLLRMPDEESGALRDLVERCAPFGTRI
                     SVEKGEAVIAL"
     gene            complement(10889..12046)
                     /locus_tag="AC16CX_13195"
     CDS             complement(10889..12046)
                     /locus_tag="AC16CX_13195"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3013503.1"
                     /translation="MKITSVRSYTVHPGWRKNLIFVKVETDAGVHGWGEAYSQYDRDT
                     AIAAQLESLGRYAVGRSPLDIKHFTQIAFDDYAQRRGSVELFCAISGIEQALWDIAGK
                     ACKQPVYNLLGGRCRDKIRVYANGWSYGLKEPADYARAAEKVVEQGFDALKLDPLPSP
                     WRTWIPREHEARAVSVVKAVREAVGPKVDILIDQHRRLAPMHAIRLDKRLAEFGLYWM
                     EEPNLAEMPEALAEVRRETGLPIVIGEATYTKTGFRPLLEARCADILNPDVACCGGIL
                     ELKEIAAMAEPFLVAVSPHNYNSTLVALASTVHVSATMPNFIITEYFLPFVEFCDRIS
                     PNQLKPKAGMIPLPTAPGLGVDVNEDALAQHAGKPYPLRALRRPEDEPGGI"
     gene            12176..>12733
                     /locus_tag="AC16CX_13200"
     CDS             12176..>12733
                     /locus_tag="AC16CX_13200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3013504.1"
                     /translation="MSTSVLGGALPQTETGLQDLVTALLPQGFAKIVQPIASTTITTD
                     TRGLIAGEVRIQTMTGAVPAYRALPEKGGKLPVVLVVQEIFGVHEHIKDVCRRLAKLG
                     YFAISVELYYRQGDVTKLTDNQEIFAKVVNHVPDSQVMSDLDAAVVYAESTGKADTAR
                     LGITGFCWGGRLAWTYCVHNPKVKAG"
BASE COUNT         2045 a         4197 c         4039 g         2452 t
ORIGIN      
        1 cttcgccgcg tcttcgcagg cggcgcatta taccggatca gaacagcccg accaccttgc
       61 cgtcatcggc gagatcgatt ttctccgccg acggcacctt gggcagcccc ggcatggtca
      121 tgatgtcgcc gcagatcatc acgatgaact cggcgccggc ggcaagccgc acctcgcgga
      181 cgttcaccat gtagccggag ggcgcgccgc gcaggcttgc gtcggcagag aacgagtact
      241 gggtcttcgc cacgcagacc gggtagtggc cgtagccgtc gtcctgcagc ttcttgatct
      301 gcgtgcgcac cttcccctcc gccgcgacgt cggccgcgcc gtagatcttg gtggcgatgg
      361 tcttgatctt gtcccacagc ggcagcgcgt cgtcgtagac aaacttgaaa tcggtgggga
      421 cgttttcgat gagattgacc acggtgcgcg cgacctcctc ggcgcccttg ccgccgtcgg
      481 cccagtgggt ggcgaggatc accggcgctt cgtggtgcgc catctttttc ttgagcagct
      541 cgatctcggc gggcgtgtcg aagttgaagc ggttgatcga gaccacgcac ggcaggccgt
      601 agtgattgcg gatgttgttg acatggcgct caaggttggc gatgcccttc tccagcgccg
      661 cgaggttttc cttgttgact tccttgatgt tgacaccgcc gtgatacttg agcgcgcgga
      721 tggtggcgac gatgaccacc gcgtccggcc tcaatccggc cttgcggcat ttgatgtcga
      781 tgaatttctc cgcgccgaga tcggcgccga agccggcttc cgtcacgcag tagtccgcga
      841 gcctgagggc ggtccgggtg gcgatgaccg agttgcaacc gtgggcgatg ttggcgaacg
      901 gcccgccgtg gatgaaggcg ggattgttct cgagcgtttg caccagattg ggtgcaagcg
      961 cgtctttcag cagcaccgtc atcgcgccgt ccactttcag gtcacgcgca cgcaccggct
     1021 tctggtcgcg ggtgtagccg acgacgatgt tgccgaggcg gttcttgagg tccttgagcg
     1081 aagtcgcaag gcagaagatc gccattactt cggaagccgg cacgatgtcg aagccgtcct
     1141 ggcgcgggta gccgttgcca acgccgccca ggccgaccgt gatgtcgcgc agcgcgcggt
     1201 cgttcatgtc catcacgcgg cgccaggtga tgcggcgcac gtcaattcca agctcgttgc
     1261 cgtggtggat gtgattgtcg atcgccgcgg cgagcaggtt gttggcgagc gaaatcgcgc
     1321 cgaaatcgcc ggtgaaatgc agattgatgt cctccatcgg caccacctgc gcgtagccgc
     1381 cgccggctgc tccgcccttg atgccgaaca cggggcccag gctcggctcg cgtatacaca
     1441 gcaccgtctt ttttccgatg cggttgagcg cgtcgcacag gccgacggtg gtcgtggtct
     1501 tgccctcgcc cgcgggcgtg ggcgtgatcg cggtcaccag gacaagcttc ccgtccttgc
     1561 ggttgttcag gctgtcgacg aagtcgagcg agaccttcgc cttgtagtgg ccgtagggca
     1621 cgagatgctc ttccgcgatg ccgagcttcc cggcaacctg ggtgatgcgc agcatcttcg
     1681 cccgctgcgc gatttcgatg tccgacaatg gcatgttgtt tttcctctgt aattgctgac
     1741 aattggttgc caagacgcca agagcgccaa ggttatctgc cttgtatatc accgccggct
     1801 tgcgctggcg gtggattgct gccggggatt accttgttgt gactttgact ttcttggcgt
     1861 ccttggcggc tattttctag actctctcca gctccgcgag cgcccgcccg ccgccgtagc
     1921 tcgagcgcgg catgagctcc ccgccgcgca tgaccttgac cgcgcagtac ttgaactccg
     1981 gtatcttgcc gaaaggatcg agcgcgggat tggtgagcag gttcgccgcc gcctcgtaaa
     2041 agcagaacgg gatgaacacg gcgccgcgcg gcgtgccgtc gtcggcccgc gcatagagcg
     2101 agatgatgcc gcgccgcgat tccaccgtga tgacatcgcc gggcttagcg ccgagcgcct
     2161 cgatatccag cgggtgcagc gacgcggtcg gctccggctc gatcgcgtct agcactgcgg
     2221 cgcggcgcgt gattgcgccg gtgtgccagt gctcgagctg gcggccggtg atgagcacaa
     2281 acgggtagtg ccggtcgggc cgctcgttgg ccggaatgat gtcggccggc acgaactttg
     2341 cgcgtcccgt cggcgtcggg aatttttccg taaacaccac cggctggccg gggtcgcctt
     2401 ccttctcgca tggataggtg acgcagcttt ccgcttccag ccgctgccag gtgatccccg
     2461 cgatcgaggg catcgccttg cgcatctcgt tgaaaacgtc cgccgcgccg ccgtagttcc
     2521 agtcgagtcc gatgcggcgc gcgatctctt ggatgatcca gagatcgggc ctcgcctcgc
     2581 ccggggcgtc cagcgctctt cgaccgagct gcaccatgcg gtcggtgttg gtcacggtgc
     2641 cgtctttctc cggccacgcg gtcgcgggca ggatcacgtc ggcgtaatag caggtctcgg
     2701 tgaggaagat ttcctgcacc accagccagt cgaggcgggc gagtgcctcg cgcgcgtgat
     2761 gcgcgtcggg atccgacatc gcagggttct cgcccatgac gtacatgccg cggatcttgc
     2821 cgtcgagcac cgcgtgcatg atctcgacca cggtgaggcc gggtttggga tcgagcgtcg
     2881 tgccccacag cttctcgaag cgcgaccggg cgtcgttgtt gtccacgcgc tgatagtccg
     2941 ggaacaccat cgggatcagg cccgagtcgg aggcgccctg cacgttgttc tgcccgcgca
     3001 gcggatgcag cccggttccc ggccgcccga tctggccggt catcaagcac agcgcgatca
     3061 ggcagcgggc gttgtccgtc ccgtgcacgt gctgcgagat gcccatgccc cacaggatca
     3121 tcgaggccct cgatttcgcg tagagccgcg ccacctcccg gatggtcccg gccgggatgc
     3181 cgcagaccgg cgccatcgcc tccgggctgt agcccgcgac gttcttctgc agttcctcga
     3241 aaccgatggt gcggcccttg atgaaatcct cgtccaccag accctcgtgg acgatggtgt
     3301 gcatcatggc gttcagcagc gcaacgtcgg tatcgggctt gaactggaga tagtacgcag
     3361 cgtggcgcga cagatccgag cggcgcggat cggcgtagat cagcttggcg ccctgcttcg
     3421 ccgcgttctt gatccaggtg gcggcgaccg ggtggttcga caccgggttg gcgccgatca
     3481 ggaaaatcac ttccgcgcgg gcgacatcgt tcacctggtt cgacaccgcg ccggaactga
     3541 tgccctccat cagcgccgcg acgctggatg cgtggcacag ccgcgtgcaa tggtccacgt
     3601 tgttcgagcc gaagccggtg cgcaccagct tctggaacag gtatgcctct tcgttggtgc
     3661 ccttggccga gccgaatccc gccagcgcgt gcttgccatt ttggtcgcgg atttccttta
     3721 gacggccggc ggcgagatcg agcgcctcct cccacgtcgc ttcgcggaaa tactcgaggg
     3781 ggttggccgg atccatcgtg aagtcggcat gtttcggcac cccgggcttg cggacgaggg
     3841 gcttcgtaag ccgctgcttg tggtgcacgt agtcgaagcc gtagcgtccc ttgacgcaca
     3901 accgcccgtg gttggacggg ccatcgcgcc cgtcaacgta aaggatcctg ttgtccttca
     3961 cggcataagt aagctggcag ccgacgccgc agtacgggca taccgagtgc acctgcttgt
     4021 cgggcttcac catgcccacg ccgcgcgccg gcatgagcgc ccccgtgggg caggcctgca
     4081 cgcactcgcc gcaggccacg caggtggact cgcccatcgg gtcgtcgagg tcgaacacga
     4141 tcttcgagtg ctcgccgcgg aaggcgtagc cgatgacatc gttcacttgc tcctcgcggc
     4201 aggcgcgcac gcaacgcgtg cactggatac agtggtcgag gttgaccgcg ataccgtagt
     4261 gcgagagatc cggcttgggc tggtgccgcg gcgcaaagcg cggcttgccg accttgagct
     4321 tggccgccca tcggtcgagc tcggaattca gggtgtgtcg ccgtttcgga atgtcggaga
     4381 gcaacagctc cagcaccatc ttctgcgagg caagcgagcg cgcgttgtcg gtgaagacct
     4441 ccatgccctt gcttggcttg cggcagcacg agggcgtaag cacgcgctcg cccttgatct
     4501 cgaccatgca ggcgcggcag ttgccgtcgg gacgcatgcc ttccttgtag cagaggtgcg
     4561 ggatctcgat gccgtatcgc ttggcggtct ggatgagggt ttcgttcgcg tcaccgacca
     4621 cctcgctgcc gttcagcttg aattcgaccg gcatcgccgc ttgctcttcc ttgctcatcg
     4681 ccgtcataag ctcgctccgc ttgcagccgt cagcagccag ttatcagcgt tcagccagac
     4741 caaacgtacc tggctgaaag ctgacggctg acagctgagg gctttacttg atctcatggg
     4801 ggaagtattt caccacacat cgtatgggat taggcgccgc ctgccccagc ccgcatatcg
     4861 atgcgtccgc catcgccccg gaaagttcct caagcaggcc ctggtcccag cgcggcgcct
     4921 gcatcagagt gagcgccttg gcggtgccca cgcggcacgg cgtgcactgg ccgcaggatt
     4981 cgtccgcgaa gaacttcatc acgttggccg ccgcggcccg cgcctggtcc cggtccgaca
     5041 ggatgatgac cgcggccgag ccgatgaagc agccgtactg attgagggtg tcgaaatcga
     5101 gcgggatgtc gcccatcgag gcgggcagga tgccgcccga ggcgccgccc ggcaggtagg
     5161 cgtagaaggc gtgccccggc agcatcccgc cgcagtattc ctcgatcagc tccttcaccg
     5221 tgatgcccgc gggcgcaaca tgcacccccg gtttcttcac gcggcccgac accgagaacg
     5281 agcgcagccc cttgcggccg tgccggccct gggaggcaaa ccaggccgca cccttctcca
     5341 ggatctcgcg cacccagtag agcgtttcca tgttgtgctc gagcgtggga tggccgaaca
     5401 gcccgacctg cgccacgtac ggcggccgca accgcggcat gccgcgcttg ccttcgatcg
     5461 attcgatcat cgcggattcc tcaccgcaga tgtaggcgcc cgcgccgcgc cgcaggaaaa
     5521 gcggcggcag cgcgcacggg ggatcggcct gcaacgcctt cagctcgcgt tcgaggatct
     5581 cgcggcagcc ggcgtactcg tcgcgcaggt agatgtagac ctgcgcgatc tgcacggtcc
     5641 acgcggcgat gatcgcgcct tcgagaaaac gatgcggatc gcgctcgagg tagtagcggt
     5701 ccttgaaggt tcccggctcg ccctcgtcga ggttgatcgc gagcagccgc ggcgcctgct
     5761 ccgccctcac gatgcgccac ttgcgcccgg cggggaagcc cgcgccgccg agtccccgca
     5821 agccggagtc ttccatcgtc ttcatgatgc tctcggcgtc gcgcttcccg gcgaggcatt
     5881 ccgcggcgat ccgatagccg cccttcttcc ggtactccgc gtaggtgatg tagcgggaga
     5941 ccggacactg ggtcttcccg gcggccgcca gcggcttcac tttccctacc gtcgcctgcg
     6001 ccacggggtt ttgccccacc accgcgaccg gcgcctgctc gcagcgcccg acgcaagggg
     6061 cggggatcat gcgcacgctc gggccgagcg cacgcttgag ctcaccgatc aactcgtggg
     6121 cgccggccag atcgcaggag agcgaatcgc acacccgcac ggtgagcggt gggggcgctg
     6181 tctcgccctt cttgacgacg tcgaaatggt gatagaaggt cgcgacttcg tagacttcgg
     6241 ccggcgcgag gttcatctcg cgcgcgagcg caacgaggtg ggccgctgac agttggccgt
     6301 aacggtcctg gatcttgtgc aggtgctcga ccaggaggtc gcggcgccgc ggtccgtctc
     6361 ccagcagcgc ctgcacctcg gccagcgcgc gcgggtcgac caggcggccc ttgggcttgc
     6421 cgcgcgcgtc cccgtcgcgc cgtttcttga cgataccgcc ctccaacgtc cctccttgca
     6481 tgcgggccat aaacggtccg cacgacagaa actccccgat tatttcaata ccgacttgag
     6541 caacctgccc atctccgctg ggttgcgcgt gattttgatg ccgcactctt ccatcaccgc
     6601 cagcttctcg gccgccgtgc ccttgccccc ggagatgatc gcgcccgcgt gccccatgcg
     6661 cttgcccggc ggcgcggtca cgcccgcgat gaatccgacg atgggtttct tcatgttcgc
     6721 cctgacccag cgcgccgctt cttcctcgtt ggtgccgccg atctcgccca ccatgaccac
     6781 ggcttcggtc tgcgggtcgt cgttgaacat ctccagcacg tctacgtgct tcaggccgtt
     6841 caccggatcg ccgccgatgc ccacgcaggt ggactggccg aggcccgctt ccatcagctg
     6901 gcccacggct tcgtaggtga gcgtgccgga acgcgacacc acaccgaccg gccctttcct
     6961 gtggatgtgg ccgggcatga tgccgatctt gatctcgtcc ggcgtgatca ccccggggca
     7021 attggggccg accagcagcg ttttgcttcc ttgcatcttg tagcgggtgc ggatcatgtc
     7081 tcgcaccgga atgccctcgg tgatgcagat gacgagctcg agccccgcct ccaccgcctc
     7141 gtcgatcgcc gcggcggcga aggggggagg aacatagatc acggacacgg tggcgccggt
     7201 tttctccttc gcctccctca ccgtttcgaa gatcgggatg ccctcgaaac tctggccgcc
     7261 cttcttcggt gtcacgcccg cgacgtagca gcgggcgccg tttgcgtact ccttgcccat
     7321 cttggtgtgg aactggccgg tcttgccggt gatgccttgc gtcatcaccc gggtgttcct
     7381 gttgatcagt atgctcataa cgcgtcgcca aagaacggaa gtgagtggtt actctccccg
     7441 gctcatccaa gagaatgaat accccctatc gcccgatcac tcgttcactt tccccgggcg
     7501 gccgcgacca ccttttgagc ggcatcggcc atgttgttgg cggagatgat gggcaacccc
     7561 gacccggcga gtatcttctt gcccagctcg acgttggtgc cctcgagccg caccaccagc
     7621 gggaccttga ggctcacctg gcgcgcggcg gtgaccactc cttcggcgat gacgtcgcac
     7681 ttcatgatgc cgccgaagat gttgaccagg atcgccttca gcccggggtt gtgcagcatg
     7741 atcttgaacg cctccgtcac cttttcggtg gaggcgccgc cgccgacatc gaggaagttg
     7801 gcggggctgc cgccgtagag cttgatgatg tccatggtgg ccatcgcgag ccccgcgccg
     7861 ttcaccaggc agccgatgtt gccgtcgagg gagatgtagg aaagatcgaa cttggaagcc
     7921 tcgacttcgg ccgggtcttc ctcgtcgagg tcgcgcatgg cgacgatctc gggatggcgg
     7981 aacaagccat tgtcatcgaa gttcatcttg gcgtcgagcg cgagcacgcg ctcgtctccg
     8041 gtcacgatca gcggattgat ctcggcgagc gaggcatccg tctcgtcgaa tgcacggtac
     8101 agtcccttca agaagtcgcg cgcttgcggg acggccggtt gcggaacgcc gatcttgcgc
     8161 gcgatttctt ccgcgtcccg gtcggccagc cccatggcgg gatcgatcgt caccttgtga
     8221 attttttccg gcgacttcgc cgcgacctcc tcgatgtcca tgccgccttc gctggaagcc
     8281 atcaggacca cccgctgcga gccgcggtcc accaccatgc cgaggtagag ttccttcctg
     8341 atgtcggccc cttcctcgat cagcagccgt cgcaccttct ggccttgcgg gccagtctgg
     8401 tgcgtcttca actgcatgcc cagaatctgc gcggcgtggc gctgcacctc ttcgagcgat
     8461 tttgtgagct tgaccccgcc gcccttgcca cggccgccgg cgtggatctg ggccttgacc
     8521 acccacacgc ttccaccaag cttcttcgct gcctccaccg cctcctccac gctgaaacag
     8581 gggtaaccgc gaggcgtggc gatgccgtat ttgcgcagga tttccttggc ctgatactcg
     8641 tggattttca tctgtgcccg tgtcgttcgg cccgtcgctg cgcgccggtc aggactgtat
     8701 tacagccctt tgcgcgccgg ccttgaccga ggtcaatatc cgggggggac cctctccgga
     8761 agctgtgtgg ggcgcatctt gtccggtgcg cgcgccacgg tgtcgagcgg ggcagtataa
     8821 caaaccgcgt cggcgtatcc ggtcctaccg gacgggtcgc tcacaagcaa tgcctctgcg
     8881 gttacagctc tcgcgttcgg ggcgccgacg cggttatagc gcgcgaccct gttcccaatg
     8941 aaaaccctgc tggaactctc cgggatgttg cgtcgcatta atcacggtga gctgcgagga
     9001 acaacatatc caatgtgcat ctatcgccgc gattgggggc aaaaaaaagc gcaacccgtg
     9061 agggttgcgc ttaagttcca ccaaaggagg aggatttgga ggagtgtgcc gacagggtgt
     9121 cgacacgaac tcattatgct gacttccaag acaaaatgca agcgtttact gctgctctgc
     9181 aatacccgac gcctaaagcc atcgatgcta catctacaaa attattatta tcaaatagtt
     9241 atgactatct aacgatgagg acaggtatct tggcatgggt taaaacttta cgggtctcgc
     9301 tgcccagcag cacagctgca agccccttgc gtccatggga tgtcatcacg atgagatcgc
     9361 agtcgcgcgc ttccgcgctc ttcagaatcg cttcgtaggg atgatcgctg gtttcacaca
     9421 gggtatcgca gggcacgccc gcgtcctcgg cggtcttgcg cacgaacgcc agataaccct
     9481 ccgcgcgcgt cttcgcttct tcctcgatgc gctcctcggt gaccggatca aaggccccct
     9541 catacgcgat caggagatgg taatcgggga tgacgtgtat gccggtgatc cgggcctggt
     9601 gcagcttcgc gagccccacc gcggtgcgca ccgcccgctg cgagagctcc gaaccgtcag
     9661 tcggtatcaa aatattcttg aacatcggcg cctcctccgc gctgccgtga ccgggttcca
     9721 gcatctggac ccggcgtcaa gcttgtcgtt cctcgactgg gctgtcaagg gtttcggagc
     9781 gcatcctcgc gccacgcatt ccctggggcg ttcgctataa tccgatcggc ttgcaacccc
     9841 ccgttcctca tcccatgcgg caccagttgc ttctcaccgg cgacgtcaat ctcatgaaag
     9901 tgggcgaccc gcgcgtgccg ttcgcgcgcg tcgccgaaac gttgcgcaca gccgacctgg
     9961 ttttcggcaa tctcgaatgc tgtctgtact cgccaccggc cgagcgttcc ctcgccgacg
    10021 agggcttcta cgcgccgccc gccgcgggcg aagcgttgag gctcgccggc tatcacgtgg
    10081 tcggcaccgc caacaacgtg aactacggtt tcgaggcgat cacgcattcg ctgcgagagc
    10141 tggaccggct cggtatcgcg cacgccggca gcggcgccaa ccgcgtcgcc gcgcacgcgc
    10201 ccgcggtcat cgagcgcaac gggttgcgtt tcgggctttt gcagcgcacc tcggtgtact
    10261 ggccgaccga ccacgaggcg ggcgaacact cggccggcgt cgccgtgatc cgcggtcaca
    10321 ccgcgtacca ggtgccggcg cacaagaccc ggcccgagat cccgccgatg aaccgcccgg
    10381 gcgtgccgcc ggtcatcctg acctgggccg acgcggatta cctcgcgcgc tttcgcgagg
    10441 atctcaccgc gctgcgcggg agagcggata tcgtggtggc ctcgcatcac tgggggttgc
    10501 accaggaggt gctccagtac atgaccgaag tcgcgcatat ggcgatcgat gccggcgccg
    10561 acctcgtggt cggacatggc ccgcacttcg tcctgccggt cgaagtctac aagggcaagc
    10621 cgatcttcta tgggatgggc aacttctcgt tccataccgg ccacggcggc cgcaagcacg
    10681 gcgactgggt gggcctgctg gcgcgcgtcg agctggagag tcgcgcgatc aggcgcgtcg
    10741 cgctcacctt cgtgcgccac aacgaccgca acgagacgct gctgcggatg ccggacgagg
    10801 aatcgggggc gttgcgtgat ctcgtcgagc gctgcgcgcc cttcggcacc cgcatctcgg
    10861 tggagaaggg cgaggcggtg atcgcgcttt agatcccgcc cggctcgtct tcgggacggc
    10921 gcagcgcgcg cagcggatag ggcttgccgg catgttgtgc cagcgcgtct tcattcacgt
    10981 ccacgcccag ccccggcgcc gtcggcagcg ggatcatgcc ggccttcggc ttcaactggt
    11041 ttggcgagat ccggtcgcag aattcgacga atggcaggaa gtactcggtg atgatgaagt
    11101 tcggcatcgt ggcggaaacg tgcaccgttg acgccagcgc gaccagggtc gaattgtagt
    11161 tgtgcggcga caccgcgacg agaaacggtt cggccatcgc ggcgatctcc ttcagctcca
    11221 ggatcccgcc gcagcacgcc acgtccggat tgaggatgtc ggcgcagcgg gcttccagca
    11281 gcggccggaa accggtcttg gtgtaagtcg cctcgccgat cacgatcggc aatcccgtct
    11341 cgcggcgcac ttccgccaag gcctccggca tctccgcgag attcggctct tccatccagt
    11401 acagcccgaa ctcggcgagc cgcttgtcga gccggatcgc gtgcatcggc gcgagccgcc
    11461 ggtgctggtc gatcaggatg tccaccttgg ggcccaccgc ctcgcgcacc gccttcacca
    11521 cgctgacggc acgcgcttcg tgctccctgg gaatccaggt gcgccacggg ctcggcagcg
    11581 gatcgagctt gagcgcgtcg aagccctgct cgactacctt ctccgccgcc cgcgcgtaat
    11641 cggcgggttc cttcaggcca tagctccagc cgttggcgta gacgcggatc ttgtcgcggc
    11701 agcgcccgcc gagcagattg tagaccggct gcttgcaggc cttccccgcg atgtcccaca
    11761 gcgcctgctc gatgccggaa atcgcgcaga aaagctcgac cgagccgcgg cgctgcgcgt
    11821 aatcgtcgaa ggcgatctgc gtgaaatgct tgatgtcgag ggggctcctg cccaccgcgt
    11881 agcggccgag cgactcgagc tgcgccgcga tcgcggtatc gcggtcgtac tgcgaatagg
    11941 cttcgcccca gccgtgaacg ccggcgtcgg tctcgacctt gacgaagatc aggttcttgc
    12001 gccatcccgg atggaccgtg tagctcctga ccgaagtgat cttcatcgtt gcgccggctc
    12061 gtgaaaggag aggagagcgg gtgaatctat gatgcgccgc tttgacgtgt caagcgcggt
    12121 gctatactca cgcgcgactt ttcccgccat cgtaatttcc gaacctggag aaatcatgag
    12181 cacatccgta ctgggcggcg cgctgccgca aactgaaacc ggactgcagg atctcgtcac
    12241 ggcgttgctg ccgcagggct tcgcgaagat cgtgcagccg atcgcgtcca ccaccatcac
    12301 gaccgacacc cgcgggctca tcgccggcga agtgagaatc cagacgatga ccggggcggt
    12361 gcccgcgtac cgcgcgctgc cggaaaaagg aggcaagctc ccggtcgtgc tggtggtgca
    12421 ggaaatcttc ggcgtgcacg agcacatcaa ggacgtgtgc cgccggctcg ccaagctcgg
    12481 ctacttcgcg atctcggtcg agctctacta ccgccagggc gacgtcacga aattgaccga
    12541 caaccaggag atcttcgcca aggtggtgaa ccacgtgccg gacagccagg tcatgtcgga
    12601 cctcgacgcc gccgttgtat atgccgaatc caccggcaag gccgacacgg cgcggctcgg
    12661 catcaccggt ttctgctggg gcgggcgcct cgcctggact tactgcgtgc acaacccgaa
    12721 ggtgaaagcg ggc
//
LOCUS       DBNGWZ010000436         5044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_306531, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000436 DBNGWZ010000000
VERSION     DBNGWZ010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_306531"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC16CX_13205"
     CDS             <1..382
                     /locus_tag="AC16CX_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013505.1"
                     /translation="IPGNDMTFPGIKDDKARRDLIAYLKAADTTPGMQRMSPRMPNLK
                     KAKPDSIVKAIRHCGDTYFVTTEDGKTEKIWEFNLRFKTDTSDTGPAPGRPVLIGVGM
                     RGDRAAIIFAGPSEIGALVKEQCS"
     gene            648..1037
                     /locus_tag="AC16CX_13210"
     CDS             648..1037
                     /locus_tag="AC16CX_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013506.1"
                     /translation="MRAILLRHLLRRLALPLAGLLLFGQLALATQACMLRPQSGSMQM
                     SSDAMVMDECIGVPADTITRLVNCLKAEQPANPSADYHFPAILPPLTAVVGLSAPWQL
                     HSSAIPSPVARYPGAPPLQILFCSYQV"
     gene            1204..1440
                     /locus_tag="AC16CX_13215"
     CDS             1204..1440
                     /locus_tag="AC16CX_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013507.1"
                     /translation="MKALTAILLTGAALVVATPALAGRDESQMMLLQQAIAKQKAERV
                     QQAQTGLAGPVGMTGKIGPGTQPVKARRDPTAHP"
     gene            complement(1529..2428)
                     /locus_tag="AC16CX_13220"
     CDS             complement(1529..2428)
                     /locus_tag="AC16CX_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013508.1"
                     /translation="MLPAHMRLVRTFTSFAAAFLIGESALAADLAPPSRIAIQVEHGD
                     WGTARAQDIEKVLGSVAGVLLPSFPQRASERVVVAFNRQGPRVLLEKSPDGAHRVFLN
                     VQDTRWDQFAYQFSHELCHIFTNFEHREISRDAASRDHQWFEEALCEAVSLFTLNRLA
                     SSWERSPPYPGWEQYAPAFREYVNLLLSKRHRHLQSSETIARWYRANQMDLDGNPYLR
                     EKNEVVATRLLSLLEGMPGSLEAIGYLNLEGSPSATSLRTYLESWYDCCPEKYRAFIS
                     RVIALFEERDNSGQTAVALALSH"
     gene            2563..2946
                     /locus_tag="AC16CX_13225"
     CDS             2563..2946
                     /locus_tag="AC16CX_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013509.1"
                     /translation="MRERTGKVQQEVEQGSNAQAGEAAQDRRFSVLLDPAAWSRVYPV
                     AVGGRIVDGLTIARVARSAWLAIDTMRYYGHEKPLKRHVALHAVYFRISGAHVQGLRA
                     HEGKSAHINSFSVCLTFLSESAIRI"
     gene            complement(2886..3545)
                     /locus_tag="AC16CX_13230"
     CDS             complement(2886..3545)
                     /locus_tag="AC16CX_13230"
                     /inference="COORDINATES: protein motif:HMM:NF023287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HIR3013510.1"
                     /translation="MRTSEQERHLAECATCASLEREMEDFEARVHDAMRVPVPEGLAD
                     RVFLRRKIRAPAVRAWALAASLVAALGVGIYFYRAPVGEDERMLTAAVLGASHPAVKA
                     IAHVLEDEPQMLKENRGVDPAAMRAAFTRLGLNAPAGGTTVLYFDKCPMTGGAGEHVV
                     MQTPFGRVTVILIPDQPFASRVVVADRDKTAIAAPHGTGGYILIADSLRNVRQTEKLL
                     M"
     gene            complement(3587..4123)
                     /locus_tag="AC16CX_13235"
     CDS             complement(3587..4123)
                     /locus_tag="AC16CX_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3013511.1"
                     /translation="MARDRLSGMLSNQKKPQFEALVRAYSAELYRYAYWLCRDRFVAE
                     DLVQETFTRAWQSWEGLRDDKAARSWLYTILRNEHARLYERKRLEIDDVQELDDLVDV
                     AGMGPHEDLEMRQLLATLPVGYREPLVLQVLGGYSCAEIAGIMDISEGAVMTRLTRAR
                     LALRKLPGVVSARKVKNL"
     gene            complement(4205..4501)
                     /locus_tag="AC16CX_13240"
     CDS             complement(4205..4501)
                     /locus_tag="AC16CX_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013512.1"
                     /translation="MLKRYMLASLVAGLTLGAQSAVASDLWFYEGHGWLPQPPFSSST
                     DARDSRSPAVIAGPTSSIAGSEPRQVVKPQTGASPRVAVPAPYDKDGFDAGYFN"
     gene            complement(4706..>5044)
                     /locus_tag="AC16CX_13245"
     CDS             complement(4706..>5044)
                     /locus_tag="AC16CX_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HIR3013513.1"
                     /translation="PVPQRWRRRMLALGSGEPTRAICSTLIAQLFQSVGYPILPRIET
                     IPAPRLGCNDCVQEILHIRHYSLFAPRDFDISPYFQVVKPTLEGGFDYKSLVWDRADE
                     RFRLEKSPAA"
BASE COUNT         1043 a         1555 c         1497 g          949 t
ORIGIN      
        1 catccccgga aacgacatga cgttcccggg catcaaggac gacaaggcgc ggcgcgatct
       61 cattgcctat ctcaaggcgg cggacacaac gcctggcatg cagcgcatga gccctcgcat
      121 gccgaacctc aagaaggcga agccggacag tatcgtcaag gcgatccgtc attgcggcga
      181 tacctatttc gttacgaccg aagacggaaa gaccgaaaaa atctgggaat tcaacctccg
      241 cttcaagacc gacaccagcg ataccgggcc ggcccccggc aggcccgtcc tgatcggcgt
      301 cggcatgcgc ggcgaccgcg cggcgatcat cttcgccggt cccagcgaaa tcggcgcgct
      361 ggtgaaggaa caatgcagtt gatcccgtcc atgacccgat tctgcctcga cccacgtcat
      421 ccccaaaaac cggcgagtgc cggagaagac gcgattgcca cctccgggcg cagcatccat
      481 cactcgcgct acgccgggca cacgtaggtg cagttagcgc accacgatgg tgcgagatga
      541 gggcgaagta agccacggac gaggttacgc gcttgaccta gatcaacttt cgctgggatg
      601 aagctggacc caggaaccgc ccggtttaga atcggatcga aatcgctatg cgggcaattc
      661 tcttgagaca tctgctcagg cggctggccc tgccactggc cggtttgctg cttttcggac
      721 agctggcgct tgccacgcag gcatgcatgc tgcgaccgca atccggctcg atgcaaatga
      781 gcagtgacgc catggtgatg gacgagtgca taggcgttcc ggcggacacg ataacgcgcc
      841 tggtcaactg cctcaaagcg gaacaacccg cgaatccctc tgccgattat cactttcctg
      901 caatcctgcc gccattgact gccgttgtgg ggttgtctgc gccatggcag ttacattctt
      961 cagcgattcc atcgcccgta gcgcgttacc caggtgctcc accgctgcag atccttttct
     1021 gcagctacca ggtttaagcc gaccgtttaa cgagcgcgtc gaaaagccgc ggctcgcctg
     1081 tcgctccgga gaattggctt cgggcgcacg cactgctctt cgaccgcttt agaggcgcca
     1141 tgcaaacgat gacgaacaac cgcatggctg ggttgaagca gtaactcaaa gtggaggcat
     1201 atcatgaagg cacttacagc aattcttctc actggtgcgg cattggtcgt ggccacccct
     1261 gctcttgcgg gccgcgatga gtcgcagatg atgttgttgc aacaagcgat cgcgaagcaa
     1321 aaggctgaaa gagtgcagca agcacaaacg gggttggcgg gacccgtcgg catgacaggc
     1381 aagattggac ccggaacgca accggtcaag gccagaagag atccgaccgc tcacccgtaa
     1441 ataacagcgg ccgttgaaag aggaacagcc cgggataccg ggctgttttt ttatgcgcgc
     1501 ctttgagcgc gccgatcgca acaggcgact aatgtgaaag ggccaaagcg acagcggttt
     1561 gaccgctatt gtcgcgctcc tcaaaaagag cgatcacccg gcttatgaac gctcggtatt
     1621 tttccgggca gcagtcatac cacgactcga gatacgttct caggctcgtg gcggagggcg
     1681 acccttccag gttcaggtat ccaatggctt cgaggctgcc gggcatgcct tcgagcaagg
     1741 aaagcaacct tgttgcaacg acttcgtttt tctccctgag atacggattg ccgtcgagat
     1801 ccatctgatt agcccgatac caccgtgcga tcgtctcgct cgactgcaag tgccggtgcc
     1861 ttttgctcag cagaaggttc acatactcgc ggaatgcagg ggcgtattgt tcccagcccg
     1921 gatacggtgg tgagcgctcc caactcgatg ccagccgatt gagcgtaaac agcgacaccg
     1981 cttcacacag cgcctcctca aaccattgat ggtcccggct tgcggcgtcg cggctgattt
     2041 cccgatgctc gaagttggtg aagatgtggc acagctcgtg cgaaaactga tacgcgaact
     2101 gatcccaacg cgtgtcctgg acgttcagaa agacccggtg cgcgccgtcc ggagatttct
     2161 cgaggaggac ccgtggccct tgcctattga acgccaccac cactcgttcc gaggcgcgct
     2221 gcggaaaaga aggcagcagc acgccggcga cggagccgag aaccttttca atgtcttgtg
     2281 cccgcgccgt tccccagtcg ccgtgctcga cctggatagc gattcgagag ggtggcgcca
     2341 aatcggctgc gagtgccgat tcgcctatca agaaagccgc tgcaaagctc gtaaaggtcc
     2401 gaactaatcg catgtgcgct ggaagcattc cggttccacg agggtttacc cagcaactag
     2461 cgggccatgc agtgatgagc cactaattca ttgatagaat gaagaaagta ttgaggatct
     2521 ttgagactag cctgtttcat tcgccctaaa ctggtgcacg gaatgcgcga gcgcaccggg
     2581 aaagtgcaac aggaagtcga acagggttca aacgcccagg caggtgaagc cgcacaagac
     2641 cgacgttttt cggtgctgct tgaccctgcg gcatggtcca gggtttatcc agtagcggtt
     2701 ggaggacgaa tcgtggacgg attgaccatc gcccgagtgg cgcgcagtgc ctggctggcg
     2761 atcgacacca tgcgctatta cgggcatgag aaaccgctca aaaggcacgt cgcactgcat
     2821 gcggtgtatt tccgtatttc cggcgcgcac gtgcaaggat tgcgggcgca tgagggaaag
     2881 tccgctcaca tcaacagctt ttcggtctgc ctcacgttcc tgagcgaatc agctataaga
     2941 atataaccac cggtcccatg cggggccgcg atcgcggtct tgtcccggtc cgcgaccaca
     3001 acgcgtgagg cgaacggctg gtccggaatc aggatcacgg tgacccgtcc gaatggcgtt
     3061 tgcattacga cgtgctcgcc ggcaccgccc gtcatcgggc acttgtcaaa atagagcacc
     3121 gtcgtcccgc cggcgggagc gttcaggccg aggcgcgtga atgccgcgcg catcgctgcc
     3181 ggatcgacac cgcgattctc cttgagcatc tgcggctcgt cctccagtac atgggcgatc
     3241 gccttcaccg cggggtggct ggcgccgagc accgctgccg tcagcatgcg ctcgtcttct
     3301 cccacgggtg cacgataaaa atagatcccc acacccagcg cggcgaccag ggaagccgcc
     3361 agcgcccacg cgcgcaccgc cggcgcgcgg atcttgcggc gcagaaacac gcggtccgcc
     3421 agtccttccg gtaccggcac gcgcatcgca tcgtggacgc gggcttcgaa gtcctccatt
     3481 tcacgctcca gactcgcgca ggtggcgcac tccgcaagat gccgttcctg ctcggacgtt
     3541 ctcatacgag ggtccgcgag caacagtttc cggaattcga agcagttcat agatttttta
     3601 ccttgcgcgc cgagactacg ccgggcagtt tccgcagagc cagtctggcg cgcgtcaagc
     3661 gtgtcataac cgccccctcg ctgatgtcca tgattcccgc gatctccgcg caactgtaac
     3721 cgcccaacac ctgcaggacc agcggttcgc ggtagccgac cggcagggtc gcgagaagtt
     3781 gtcgcatttc caggtcttca tggggaccca tcccggctac gtccaccaga tcgtccagtt
     3841 cctgcacgtc atcgatctcc agacgcttgc gctcgtacag ccgcgcgtgc tcgtttcgaa
     3901 gaatggtgta aagccagctc cgcgccgcct tgtcgtcgcg caacccttcc cagctctgcc
     3961 aggcgcgcgt gaaggtctcc tggaccaggt cttcggcgac gaatcggtca cggcacaacc
     4021 agtatgcata gcggtacagc tccgccgaat aggcacggac cagggcctcg aactgcggtt
     4081 tcttctggtt gctcagcatg ccggacagcc ggtcccgtgc caagccatac cccgcgccgt
     4141 ctgctcagta agtgcgcagg gtggcgcctg atcggcgcca ccatggatac tcatgcgcac
     4201 gtgcctagtt gaagtatcca gcatcgaaac catccttgtc gtacggcgcg ggcacggcca
     4261 ctcgcggtga agcgcctgtt tgcggcttca cgacctgacg cggttcggac ccagcgatcg
     4321 aagacgtcgg cccagcgatt actgccggcg accggctgtc acgcgcatcc gtggacgacg
     4381 aaaacggcgg ctgcggcagc caaccatggc cctcatagaa ccacaggtca gacgcaaccg
     4441 cggattgggc gccgagcgtg agcccggcaa cgagtgaagc aagcatgtag cgtttcaaca
     4501 tggtaagtct cctgtcagtc agtagagttc atcggtgatt ggccgcattc gctgtcggca
     4561 cgttcggttt gccgggtgcg acgttgcctt gcctacatac caatagaccg tcgcggggcg
     4621 gaaaacctta caggcgtgcc cgcaggtctg gacgaatcag ggcacttgtg cggtgggcat
     4681 gcgttgattt aacgccggca caaattcacg ctgccgggga cttttccagt cggaatcgtt
     4741 cgtcggcgcg atcccacacc agcgacttgt agtcgaatcc gccttcgagg gtcggcttca
     4801 cgacctgaaa atagggcgag atgtcgaagt cacgcggcgc gaacaggctg taatggcgga
     4861 tatgcaggat ctcctggacg caatcgttgc agccaagacg cggcgccggg atcgtttcga
     4921 tgcgcggcag gatcgggtag ccgaccgact ggaacagttg cgcgatcagc gtagagcaaa
     4981 tggcccgcgt cggctcgccg ctgcccagcg cgagcatgcg ccgtcgccag cgctgcggaa
     5041 ccgg
//
LOCUS       DBNGWZ010000437         1601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2573796, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000437 DBNGWZ010000000
VERSION     DBNGWZ010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2573796"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="AC16CX_13250"
     CDS             complement(<1..110)
                     /locus_tag="AC16CX_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006483852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3013514.1"
                     /translation="MRTVTITRPDDWHLHLRDGEQLRAVLPDTARRFARA"
     gene            complement(207..1028)
                     /gene="rsmI"
                     /locus_tag="AC16CX_13255"
     CDS             complement(207..1028)
                     /gene="rsmI"
                     /locus_tag="AC16CX_13255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3013515.1"
                     /translation="MATPIGNLADISGRALEVLKSVDAIAAEDTRITRRLLAHYGIAK
                     RLIAVHEHNEKRVAGQVLALLAAGQSLALTCDAGTPGISDPGAALVAAARAAGHPVTP
                     VPGPNAAITALSASGFAAPHFLFYGFLPERAAARRRALADLAQLPHTLVFYEAPHRVE
                     ACVADLRAALGESRRIVLARELTKLFETLHECTLAEAGKWLAADPVRRKGEFVLIVEG
                     ADASEAQDPRAAWRALAALLDELPLRQAVDLAAKITGRRKNELYRMALAMKKRDS"
     gene            1346..>1601
                     /locus_tag="AC16CX_13260"
     CDS             1346..>1601
                     /locus_tag="AC16CX_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013516.1"
                     /translation="MASEEAVSLPAPPPPALAAPVELPPAPAARRDKPVPPAAADEDP
                     HIALLLPLNSKSFGKHAEAVRDGFLAAAKVQRGAALPVRTY"
BASE COUNT          251 a          550 c          524 g          276 t
ORIGIN      
        1 atggcgcgcg cgaaacgacg cgctgtatcc ggcagcaccg cgcgcagctg ctcgccatcg
       61 cgcagatgca ggtgccagtc gtcgggacgc gtgatcgtta ccgttctcat ccgggaagca
      121 atggtagtgg taacggcgta aatcgtgaat cgtgattcgt gaatcgtgtg ccgctgtcca
      181 gttcgggtcg aatcacgaat caccaatcac gaatcacgct tcttcatggc aagcgccatg
      241 cggtagagct cgttctttcg tctgcccgtg atcttcgcgg cgagatcgac cgcctgcctg
      301 agcggcagct catcgagcag cgccgcgagc gcgcgccacg cggcgcgcgg gtcctgcgcc
      361 tcgcttgcgt ccgctccctc cacgatcagg acgaattccc ctttgcgacg caccgggtcg
      421 gcggcaagcc attttcccgc ttccgccagg gtgcattcgt ggagagtttc gaacagcttc
      481 gtgagctcgc gcgcgagcac gatgcgccgg ctctcgccga gcgccgcgcg caggtcggcg
      541 acgcacgcct cgacgcggtg cggcgcctca tagaacacca gcgtgtgcgg caactgcgcc
      601 aggtcggcaa gcgcccggcg ccgggccgcc gcgcgctcgg gcagaaatcc gtaaaagagg
      661 aaatgcggcg cggcgaaccc ggaagcggac aaggcggtaa tcgccgcgtt ggggcccggc
      721 accggcgtca ccggatggcc ggctgcgcgc gccgcggcga ccagggccgc gcccggatcg
      781 gagattccgg gcgtgccggc gtcgcaggtg agcgccagcg actggccggc ggcgagcagc
      841 gccagcacct ggcccgcgac gcgcttttcg ttgtgctcgt ggaccgctat cagccgcttg
      901 gcgatgccgt aatgggcgag cagccgcctc gtgattcgcg tatcttccgc cgcaattgcg
      961 tctacgctct tcaggacctc cagcgcccgc ccgctgatgt ccgcgaggtt tccaatcggg
     1021 gtggccacta catataatgt cgctctcggt ctccccccgg tgcccccatg cgctgcggcg
     1081 tgctccatcg ctggctcgta gtgcccctag gagtttgtcg gccctaggtg cgtcaaactc
     1141 ctaacgcaaa accgccccag gaaaacgagg aatgtagcaa agaccgtctc tggaccccac
     1201 tcgcatatcc cattggaggc ctagattggt cattctccga agctttatcc gaaatcatcg
     1261 ggcggttttg cttttagcag cccgtcggat gcttgcgtcc gtcgggctgc tagctgcggc
     1321 cgtactatac gggggcgcct gcgcattggc cagcgaagag gcggtgagct tgcccgctcc
     1381 gccgccgccc gcgctggcgg cgcccgtaga gcttccgccc gcacctgccg cgcgtcgcga
     1441 caagccggtg ccgcccgcgg cggccgacga ggacccgcac attgcgctgc tgctgccgct
     1501 caactcgaag tcattcggga agcacgccga agccgtcagg gatggctttc tcgcggccgc
     1561 caaggtacag cgcggcgcgg cgctgccggt gcgcacgtac g
//
LOCUS       DBNGWZ010000438         9792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3078608, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000438 DBNGWZ010000000
VERSION     DBNGWZ010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3078608"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16CX_13265"
     CDS             complement(<1..466)
                     /locus_tag="AC16CX_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013517.1"
                     /translation="MSRWWLAARIAVVGLVFGSYSATVMAADPLVLLLLRMMRDQTIS
                     TAIESGVTASQQASKPESDIAARAAPQPFSEGQWLKSLIDESFVHLGPQQREELHASL
                     VRILSDPKNDAARSAILAEFTAQAIAVRDAHRQLSRLTEADMKLIAAEARLEF"
     gene            complement(627..1007)
                     /locus_tag="AC16CX_13270"
     CDS             complement(627..1007)
                     /locus_tag="AC16CX_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013518.1"
                     /translation="MTRILLTLASLVVICPAFAINMNGFKEAPIKRLAGAERKAFRAA
                     LIQVLNEAPDRTTVEWNAPKTRFASKLTPLRSFDEGKLRCRETLVESGVRDKYQRGLY
                     VFCKNPKGDWQFKRAAPKAVKKKS"
     gene            complement(1107..1182)
                     /locus_tag="AC16CX_13275"
     tRNA            complement(1107..1182)
                     /locus_tag="AC16CX_13275"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1146..1148),aa:Glu,seq:ttc)
     gene            complement(1210..1285)
                     /locus_tag="AC16CX_13280"
     tRNA            complement(1210..1285)
                     /locus_tag="AC16CX_13280"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1250..1252),aa:Ala,seq:ggc)
     gene            1530..2579
                     /gene="ubiU"
                     /locus_tag="AC16CX_13285"
     CDS             1530..2579
                     /gene="ubiU"
                     /locus_tag="AC16CX_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis protein UbiU"
                     /protein_id="HIR3013519.1"
                     /translation="MRFPSTKTAAGDSPSPASFELVCPAGGLPALRAAVDNGADCVYI
                     GFRDDTNARNFTGLNFDETAAREGLRYAHAHGTKVLLAINTYPQPANWKRWTGAVDRA
                     AGMGFDAIIIADAGLMEYAARVHAQLRLHLSVQASATNYEAINFYHERFGIRRAVLPR
                     VLALEQVEQVVRNTPVEIEVFGFGSLCIMVEGRCALSAYVTGDSPNTTGACSPARAVR
                     WEQTPQGMESRLNGVLVDRFADGERAGYPALCKGRYTVRDETYYAIEEPASLNAMELL
                     PRLIAMGVRAVKIEGRQRSPAYVAQVTRVWRAAIDACRRRPGVFVPQPDWIQALDKVS
                     EGQSHTLGAYHRAWK"
     gene            2581..3477
                     /locus_tag="AC16CX_13290"
     CDS             2581..3477
                     /locus_tag="AC16CX_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HIR3013520.1"
                     /translation="MKISLGPLLYYWPRETVHAFYDEIAGSAADVVYLGEVVCSRRRE
                     LRPADWLAIGERLAAAGKEVVLSTQVLIESEADLRGLRKVVANGRFRVEANEWGAVRL
                     LAKRAPFVAGPHLNVYNPATLSLLTELGAARWVAPVETTRETLAGVRDALPLTMETEV
                     FAFGRLPLAHSARCFTARHHNVPKDNCGFVCRDYPQGLELRTRENHTFLTLNGIQTQS
                     AAVYNLLTEADSLRALRVDVLRVSPQAQGTAAVLSLFRRWANGEVSAADASNLVQAAV
                     PGQHCNGFWHGLPGMELGRGTV"
     gene            3505..3984
                     /gene="ubiT"
                     /locus_tag="AC16CX_13295"
     CDS             3505..3984
                     /gene="ubiT"
                     /locus_tag="AC16CX_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110142 - ubiquinone biosynthesis complex
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone anaerobic biosynthesis accessory
                     factor UbiT"
                     /protein_id="HIR3013521.1"
                     /translation="MEPLPFVVPPLVARVNAALPQTPPAAALCLVLNLARERLFPDDV
                     LRALESKRLRLQVSDAGLVLDFTVRRGWFVPWLGAGAADLVIAATAHDFLCLALRKED
                     PDTLFFARRLLMQGDTDLGLLVKNTLDAAEFPALTLADLAPHRVLCRLAGLVLRNRL"
     gene            complement(4066..5454)
                     /gene="gltX"
                     /locus_tag="AC16CX_13300"
     CDS             complement(4066..5454)
                     /gene="gltX"
                     /locus_tag="AC16CX_13300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3013522.1"
                     /translation="MIRTRFAPSPTGFLHVGGARTALFCWAYAKKHGGAFILRIEDTD
                     RERSTRESVMAILESMQWLGLDYEGPYSQMDRLARYRLVAEQLIKKDQAYHCYASREE
                     LDALREQQRARGEKPRYDGRWRPENAKRLGLKPPPGAQPVVRFRNPDEGEVTYNDLVK
                     GLITVANGELDDLVLLRADGIPTYNFGVVVDDIDMNITHVIRGDDHVNNTPRQINIYR
                     ALGATLPRYAHVPMILGADGERLSKRHGAVSVTQYRDDGYLPEALVNYLARLGWSHGD
                     EEMFSREQLVEWFDLEHISASPARFDPGKLAWLNAQYLKSADDARLAGLAEPFLAKNG
                     CDPKRGPPLARIVALLKERVNTVEELADAAVYFYRALEPSEALLRQHLGAEARPVLAE
                     LARSLEWSEWTRQHLQAALKSVAAAQNLKMNKVAMPLRVAVTGESETPSIDAVLELLG
                     REEVLGRLRRWL"
     gene            complement(5601..6443)
                     /locus_tag="AC16CX_13305"
     CDS             complement(5601..6443)
                     /locus_tag="AC16CX_13305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3013523.1"
                     /translation="MKRHIVCVTFDFDAISGFIARGQTSPSWISRGEYGPRVGAPRLL
                     ALLGKYGIKSTWFVPGHTIETYPQACEKVVAAGHEIAHHGWTHRPPASLTREQEEAEL
                     VRANDSIKKLTGRYARGYRSPSWDLSPHSIELFLKHGLLYDSSLMGDDYTPYYARSGD
                     VIELEKPAHFGKETKLVEMPIHWATDDAPHFEFVRTETTVRQGLMNANSVGENWINDF
                     LYMRDTMEWGVLTYTCHPFVIARGARLMMLERVIKTAAENGAVFMTMEQAAEEFRSRA
                     APKS"
     gene            6593..8170
                     /locus_tag="AC16CX_13310"
     CDS             6593..8170
                     /locus_tag="AC16CX_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR3013524.1"
                     /translation="MSEHLTQNWHVRKPLARSRGGVVATQNRVAGEVGARVLAAGGNA
                     VDAAVATGFALAAVEPWNSGLGGIGFMLVYSAKQGRVSVVDFGPVSPRRLDPAAYPLT
                     GGGATSRDLFNWPAVKDDRNVHGPLSFAVPGYMDGLGLALEQFGTLKLTDAMKPAIEL
                     AERGIAVDWFLTLKIATMAKELARYPTTREIWLPGGYPPVTAPGALLERLKLKGLAGT
                     LKQLAKDGRRDFYEGEIAAAIVKDVQAMGGVLGAGDLRGYRACVVEPLTAEYRDAQFS
                     LAPGLTAGPSMLHALNGLRGTRFRRNGPHADAFLAYAEVLRKAYAERLATMGDTADRR
                     DPSTTTHLNVVDRHGNMVALTQTLLSVFGSKVVLPSTGILMNNGIMWFDPRPGSPNGL
                     APARRPLTNMCPVIALHHGKPWFAIGASGGRKIFPAVLQISSFLVDHGMFLEDAFHQP
                     RIDASEGEYVALDPRLPATVRKALTEKFTTRLNELVVYPTNYACPSAVLHDAKSRENL
                     GVTDVMSPWSGAVAEGE"
     gene            8465..9310
                     /locus_tag="AC16CX_13315"
     CDS             8465..9310
                     /locus_tag="AC16CX_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013525.1"
                     /translation="MDIMSRAIGERLTVSLGQQVVVDNRGGAGGSIGAEIAARAVPDG
                     HTLLTGHIGTHAINVSLYPKVGYDPIRDFAPVTLIATLPLGLFVNASVPAQSVKELVA
                     LARAKPGSINFASAGSGGPTHMAGEMLKAMARIEIIHVPYKGNAAALTDLVAGRVQMM
                     FSNLLTAGPHARAGRLRAIAISSGKRSQQAPELPTVAESGVPGYDLTPWYGVFAPAGT
                     PRAVVMRLNREIGAIVTAPDTRERFRAQGIDLTTSTPEAFSALIKSEIPKWRKVVKES
                     GAKAG"
     gene            9485..>9792
                     /locus_tag="AC16CX_13320"
     CDS             9485..>9792
                     /locus_tag="AC16CX_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013526.1"
                     /translation="MIRLVVALVFAAATPLQAADTYPSRPVRFVVGFLPGGPSDTIAR
                     VLAGKLGEGLAQTVIVDNRAGAGGNISAEIVAKAQPDGHTLLLGTGGPLVIAPITGQK
                     V"
BASE COUNT         1693 a         3190 c         3114 g         1795 t
ORIGIN      
        1 cgaattcgag acgggcttcg gcggcgatca gcttcatgtc ggcctcggtc agacgcgaaa
       61 gctgccggtg cgcgtcgcgc accgcaattg cctgggcggt gaattcggcg aggattgctg
      121 agcgcgccgc atcgttcttc gggtcgctca gtatcctgac caggctcgcg tgcagttcct
      181 cgcgctgctg cggtccgagg tggacgaagc tctcgtcaat caagctcttc agccactggc
      241 cttccgaaaa gggttgcggc gccgcgcgcg cggcgatgtc gctttcgggt ttcgaggcct
      301 gctgcgacgc ggtgacgccg gattcgatcg ccgtcgaaat cgtctggtcg cgcatcatcc
      361 ggagcagaag cagcacgagc gggtcggccg ccataacagt ggccgagtac gagccgaaaa
      421 ccaatccgac gacggcaatg cgagcagcca gccaccagcg gctcatcggt ctttcctcca
      481 tcatccccga cgaaaaaacg ggcgtttttt ctttaaaaat atagcactag aaagggatct
      541 tggaaacatc tccaagatcc cttgattgaa caaatataat tattgttgct tacttgatga
      601 atgcgtgacc ggcaacggtc acgaattcat gatttcttct tcaccgcctt cggggcggcc
      661 cgcttgaact gccagtcgcc cttcgggttc ttgcagaaca cgtagaggcc gcgctgatac
      721 ttatcccgga cgccggattc gaccaatgtt tcgcggcacc tgagcttgcc ttcgtcgaag
      781 ctcctgagcg gcgtgagctt gctcgcgaac cgggtcttag gcgcattcca ttcgaccgtc
      841 gtgcggtcgg gcgcctcatt cagcacctgg atgagcgccg cgcggaatgc tttccgctcg
      901 gctcctgcaa gccgcttgat tggcgcctcc ttgaagccgt tcatgttgat cgcgaatgcc
      961 gggcaaatca ccacgaggga tgccaatgta agcaatattc gggtcataaa atccgatcct
     1021 ttccgtgccc ggcattatcg cccgagttgc gagcggcgtc attgttgggt tttaaattga
     1081 aaaatccgcc ggtcggcgga tttttttggc gtccccacgg ggattcgaac cccggttacc
     1141 gccgtgaaag ggcgatgtcc taggcctcta gacgatgggg actgacgaca acctgccgaa
     1201 gggttatgct ggtggaggta accgggatcg aaccggtgac ctcttgcatg ccatgcaagc
     1261 gctctcccag ctgagctata cccccacgag agagggcgca ttataccggc gcggccgtca
     1321 caggtaaacc aacgtccgcc ccgtaccgag gtcccgcgcg cggcggcgtg tgacgcgtta
     1381 gtcgcaacat ccgttttgac ctgcgtcaag ttccaaggcc tcttactgcg gtattttaac
     1441 ggcggtcacg aacgcatcgg gccgcggcgt tcgtcacgag agcatccacc atcggaacca
     1501 gcccgctcat acgccggaga gccaagcaag tgcgattccc ctccacgaaa accgcagccg
     1561 gcgacagccc gagtcccgct tctttcgagc tcgtgtgccc ggcgggcggc ctgccggcgc
     1621 tgcgggcagc ggtggacaac ggcgccgact gcgtttacat cggattccgc gatgacacca
     1681 acgcccgcaa cttcaccgga ctgaatttcg acgaaacggc ggcccgggaa ggcctgcgtt
     1741 acgcgcacgc ccacggcacg aaggtattgc tcgcgatcaa cacctatcct caacccgcga
     1801 actggaagcg ctggaccggc gccgtggaca gggcggccgg gatgggtttt gacgccatca
     1861 tcatcgccga cgccggtctc atggaatacg ccgcacgagt gcacgcacag ttgcgcctcc
     1921 acctatcggt gcaggcgtcc gcgaccaatt acgaggccat caacttctat cacgagcggt
     1981 tcgggatccg gcgggcggtg ctgccgcgcg tgctcgcgct ggagcaggta gagcaggtcg
     2041 tgcgcaatac gccggtggag atcgaggtgt tcggattcgg cagcctttgc atcatggtcg
     2101 agggccgctg tgcattatcg gcctatgtga ctggcgattc ccccaacacc accggagcgt
     2161 gctctcccgc gcgcgcggtg cgctgggagc agacaccgca aggtatggaa agccgcctca
     2221 acggcgtgct ggtggaccgc ttcgccgatg gtgagcgcgc cggttatccc gcgctgtgca
     2281 agggacgtta cacggtgcgg gacgaaacct attacgccat cgaggaaccc gcgagcctca
     2341 acgccatgga attgctcccg cggcttatcg ccatgggcgt acgggcggtc aagatcgaag
     2401 gccgtcagcg cagccccgca tacgtcgccc aggtgacgcg ggtatggcgc gcggcgatcg
     2461 acgcctgccg gcgcaggccg ggagttttcg tgccgcaacc ggactggatt caggcgctcg
     2521 acaaggtctc ggaaggccag agccacacgc tcggcgccta ccatcgcgcc tggaaatgac
     2581 atgaaaattt cactggggcc gctgctctat tactggccgc gcgaaacggt ccacgcgttc
     2641 tacgacgaga tcgccggctc cgctgcggat gtcgtctatc tcggcgaagt cgtgtgctcg
     2701 cggcggcgcg agctgcggcc cgccgactgg ctcgcgatcg gcgagcggct cgccgccgcc
     2761 ggcaaggagg tcgtgctctc gacccaggtg ctgatcgagt ccgaagccga tctgcggggg
     2821 ttgcgcaagg tcgtggccaa cggccgcttc cgggtcgaag ccaacgagtg gggcgcggtg
     2881 cgcctgctcg cgaagcgggc accgttcgtg gcggggccgc atctcaacgt ctacaatcca
     2941 gcgaccctgt cgctgttgac cgagcttggc gccgcgcgct gggttgcgcc ggtggagacg
     3001 acacgcgaga cgctcgccgg cgtgcgggac gcgctgccgc tgacgatgga aaccgaggtt
     3061 tttgcgtttg gccgtctgcc gctggcgcat tcggcgcgct gtttcaccgc ccgtcaccac
     3121 aacgtcccca aggacaactg cggcttcgtt tgccgcgact acccgcaggg cctggaactc
     3181 cggacccggg agaaccacac cttcctgaca ctgaacggga tccagacgca gtccgcagcc
     3241 gtctataacc tccttacgga ggcggattcg ttgcgcgcgc tgcgggttga tgtcctgcgc
     3301 gtgagcccgc aagcgcaggg aaccgctgcg gtgctcagcc tgttccggcg ctgggcgaac
     3361 ggcgaagttt cagcggcgga cgcgtcgaac ctggttcaag cggcggtacc cggccagcac
     3421 tgcaacggct tttggcatgg cctgcccgga atggaactcg ggcgcggcac agtctgagac
     3481 cgggcaccac cgcgggaaaa aataatggaa cccctaccat tcgtcgtccc cccgctcgtt
     3541 gcaagggtga acgctgcgct tccacaaacg ccccccgccg ccgcgctttg cctcgttctc
     3601 aacctggcgc gcgagcgcct gttcccggat gatgtcctgc gcgcgctcga atccaagcgc
     3661 ctgcggcttc aggtgtcgga cgccgggctc gttcttgact tcacggtgag acgcggatgg
     3721 ttcgtcccgt ggcttggggc cggcgcggcc gatctcgtga tcgccgcaac ggcccacgat
     3781 ttcctgtgcc tggcgctgcg caaggaagac cccgataccc tgtttttcgc gcgccggctg
     3841 ctgatgcagg gcgataccga tctaggcctg ctcgtaaaaa acacgctgga tgcggcggaa
     3901 ttccccgcgc tgactcttgc cgatctggcg ccgcaccgtg tgctgtgccg tctcgcgggc
     3961 ctcgtgcttc gcaaccggct ttaagtggta acaaaatgac attcccctcc ttgaaaaagg
     4021 aggggatact cattttgtta cgacttcctt agaccgcatt ctccattaca gccagcggcg
     4081 cagcctgcca agaacctcct cacgaccgag cagctccagc accgcgtcga tcgacggcgt
     4141 ttcgctttcg ccggtaaccg cgacccgcag cggcatggcc accttgttca tcttgaggtt
     4201 ctgcgccgcg gcgacgctct tcagcgccgc ctggaggtgc tggcgggtcc actcggacca
     4261 ttccagggat cgtgcgagct cggcaagcac cgggcgcgcc tccgcgccca ggtgctgtcg
     4321 cagcaaggct tcggacggct cgagcgcacg gtagaaataa accgccgcgt cggccagttc
     4381 ctcgactgtg ttgacgcgct ccttgagcag cgcgacgatc cgggcgagcg gcgggccccg
     4441 cttcggatca cagccgttct tggcgagaaa cggctccgcg agcccggcaa gccgcgcatc
     4501 gtcggcgctc ttcaggtact gtgcgttgag ccacgccagt ttcccgggat cgaaacgcgc
     4561 gggcgaggcg ctgatgtgct ccaggtcgaa ccactcgaca agctgctcgc gcgagaacat
     4621 ttcctcgtcg ccgtgcgacc agccgagccg cgcgaggtaa ttcaccagcg cctccggcag
     4681 atagccgtcg tcgcggtact gggtgacact caccgcgccg tggcgcttgg aaagccgctc
     4741 gccgtccgcc ccgaggatca tcggcacgtg cgcgtagcgc ggcagtgtcg ccccgagcgc
     4801 gcggtagatg ttgatctggc gcggcgtgtt gttgacgtga tcgtcgccgc ggatgacgtg
     4861 ggtgatattc atgtcgatgt cgtccaccac cacgccgaag ttgtaggtgg ggatgccgtc
     4921 ggcacgcagc agcacgaggt cgtccagttc tccgttcgcg accgtgatca ggcccttcac
     4981 gaggtcgttg taagtcacct cgccttcgtc cgggttgcgg aagcgcacca ccggctgcgc
     5041 gccgggcggc ggcttcagcc cgagcctctt cgcgttttcc ggccgccagc ggccatcgta
     5101 gcgcggcttc tcgccgcgcg cgcgctgctg ctcgcgcaag gcgtcgagct cttcccggct
     5161 ggcatagcag tggtaggcct ggtccttttt tatcaactgc tccgcgacca ggcgatagcg
     5221 cgcaagccgg tccatctggg agtacggccc ctcgtagtcg agcccgagcc attgcatgct
     5281 ctcgaggatg gccatgaccg actcgcgcgt ggagcgctcg cggtcggtgt cctcgatccg
     5341 caggatgaaa gcgccgccgt gcttcttcgc gtacgcccag cagaacagcg cggtgcgcgc
     5401 gccgccgacg tggaggaagc cggtggggct gggggcgaag cgggttctta tcattttccg
     5461 aggcgtgatt ggtgggtggt gattggtgaa tagactgaaa ccatcagcaa aatctgcatc
     5521 cgcagggatc tttgtataac accgggtacc tcacacgatg tgccggtttc aaggtccact
     5581 cactattcac caatcaccat tcacgatttt ggagcagcgc ggctccggaa ctcttccgcc
     5641 gcctgctcca tggtcataaa aacggcgcca ttctcagccg ccgtttttat gacgcgctcg
     5701 agcatcatca ggcgggcgcc gcgcgcgatg acgaacgggt ggcaggtgta ggtcagcacg
     5761 ccccactcca tcgtgtcgcg catgtaaaga aagtcattga tccagttctc gcccaccgag
     5821 ttggcgttca tcaagccctg gcgcacggtg gtctcggtgc gcacgaactc gaaatgcggc
     5881 gcgtcgtcgg tggcccagtg gatcggcatc tcgacgagct tcgtctcctt gccgaagtgg
     5941 gcgggcttct ccagctcgat cacgtcgccg ctgcgcgcgt agtacggcgt gtaatcatcc
     6001 cccatcaggc tgctgtcgta gaggaggccg tgcttgagga acagctcgat cgaatgcggg
     6061 ctgagatccc acgacggcga gcggtagccg cgcgcgtagc gccccgtcag cttcttgatc
     6121 gagtcgttcg cgcgcacgag ttccgcctcc tcctgctcgc gcgtcaggct cgccggcggg
     6181 cgatgcgtcc atccgtggtg cgcgatctcg tgacctgccg ccaccacctt ctcgcacgcc
     6241 tggggatagg tttcaatggt atggccggga acgaaccagg tgctcttgat gccgtatttc
     6301 ccgagcagcg caagcaggcg cggggcgccc acccgcgggc cgtattcccc gcgcgagatc
     6361 caggacggcg aagtctggcc gcgcgcgatg aagcccgaga tcgcgtcgaa gtcgaatgta
     6421 acgcaaacga tgtgtcgttt cactttataa agagcgtgat tagtgagtag tgaatggtga
     6481 gtcgaccaaa cccaaacccg ctgttctcca attactactc accactcact attcacgcct
     6541 tttcggctaa gattcggctt gattatatcg tctgcatcgc cctttcccct acatgtccga
     6601 gcacctcact caaaactggc acgtgcgcaa gccgctggcg cgctcgcgcg gcggcgtcgt
     6661 cgcgacccag aaccgcgtgg ctggtgaagt cggtgcccga gtgctcgcgg ccggcggcaa
     6721 cgcggtggac gcggcggtcg caaccggttt cgcgctcgcc gcggtggagc cgtggaacag
     6781 cgggctgggc ggcatcggct tcatgctggt ttattccgcg aaacaggggc gcgtttcggt
     6841 cgtggacttc gggccggtct cgccgcgcag gctcgacccc gccgcctacc cgctcacggg
     6901 cggcggcgcc acctcgcgcg acctcttcaa ctggccggcg gtgaaggacg accgcaacgt
     6961 gcacggcccg ttgtcctttg ccgtgcccgg gtatatggac ggtctcgggc ttgccctgga
     7021 gcaattcggg acgctcaagc tgaccgacgc catgaagccc gcgatcgagc tggcggaacg
     7081 cgggatcgcg gtggactggt tcctcacgct caagatcgcg accatggcga aggagctggc
     7141 gcgttacccg acgacgcgcg agatctggct gccgggcggc tatccgccgg tgaccgcgcc
     7201 cggcgctcta ctcgaacggc tgaaactcaa gggacttgcc ggcacgttga agcagctcgc
     7261 aaaggacggc cgccgcgact tctatgaagg cgaaatcgcc gccgccatcg tcaaggacgt
     7321 gcaggcgatg ggcggtgtcc tcggcgccgg ggacctgcgc ggctaccggg cgtgcgtcgt
     7381 cgaaccgctg acggccgaat accgcgacgc gcaattctcc ctcgcgccgg gactcaccgc
     7441 cggtcccagc atgctgcacg ccctgaacgg gctgcgcggc acgcgcttcc gccgcaacgg
     7501 cccgcacgcc gacgctttcc tcgcctacgc cgaagtgctg cggaaggcgt atgccgagcg
     7561 gctcgcgacc atgggcgaca ccgccgatcg ccgcgacccc tctaccacca cgcatctcaa
     7621 tgtggttgac cgccacggca acatggtggc gctcacgcaa acgctgctct cggtgttcgg
     7681 atcgaaggtg gtgctgcctt cgaccggcat cctgatgaac aacggcatca tgtggttcga
     7741 cccccgcccc ggctcgccca acggcctcgc gcccgccagg cgtccgctta ccaacatgtg
     7801 cccggtgatc gcactccacc acggcaagcc ctggttcgct atcggcgcat ccggcgggcg
     7861 caagatcttc ccggcggtgc tgcagatttc ctcgttcctc gttgaccacg gcatgttcct
     7921 cgaggacgcg ttccaccagc cgcgcatcga tgcgagcgag ggtgagtacg tggcgctcga
     7981 tccgcgcctg cccgccaccg tgcgcaaggc gctcacggag aaattcacaa cgcgcttgaa
     8041 cgagctggtc gtgtatccga ccaactatgc ctgcccgagc gcggtgctgc acgacgcgaa
     8101 gagccgcgag aacctcggcg tgacggatgt gatgtcgccg tggtccggag cggtagcgga
     8161 gggagaatag acaggactta cgatgttgaa atcaacccgc aaaacctcaa tggacaggat
     8221 taacaggatt tacaggatta attcgaaaga caaaaagaaa ttacatcatc gaatttagta
     8281 ttggctctaa tcttgttaat cttgtgaatc ctgtctattt gcttttcaaa tgaatttcag
     8341 gaccggcaga tttctagtct taattctcgg ggtcatcctg tccgttgtga tctgcgcagc
     8401 aggcgcgcag gaatatccga caaagcccat aaggcttatt gtcccgttcg ccgcgggcgg
     8461 gccgatggac atcatgtcgc gcgcgatcgg cgagcggctt accgtgtcgc tggggcagca
     8521 ggtggtggtg gacaaccgcg gcggcgccgg cggctcgatc ggcgccgaga tcgcggcacg
     8581 cgcggtgccc gacggccaca ccctgctcac cgggcacatc ggcacgcatg cgatcaacgt
     8641 cagcctctac ccgaaggtgg gttacgatcc gatccgggat tttgctccgg taaccctgat
     8701 cgccaccctg ccgctcgggt tgttcgtcaa cgcgtcggtg cccgcgcagt ccgtcaagga
     8761 gctcgtcgcg ctcgccaggg cgaagccggg gagcatcaac ttcgcctccg ccggcagcgg
     8821 cggcccgacc catatggcgg gcgagatgct gaaggcgatg gcgcgcatcg agatcatcca
     8881 cgtgccgtac aagggcaacg ccgccgcgct caccgacctc gtcgccgggc gcgtgcagat
     8941 gatgttcagc aacctgctga ccgcggggcc gcacgcgcgc gccgggaggc tccgcgccat
     9001 cgcgatctcg agcggcaagc gctcgcagca ggcgcccgag ctgccgaccg tcgccgaatc
     9061 cggcgtgccg ggctacgatc tcacgccgtg gtacggcgtg ttcgccccgg ccgggacacc
     9121 gcgcgccgtc gtcatgcggc tcaaccgcga gatcggcgcc atcgtgaccg cgcccgatac
     9181 cagggagcgg tttcgcgcgc aggggataga cctcacgacc agcacgccgg aagcgttctc
     9241 ggcgctgatc aagtccgaga tacccaagtg gcggaaggtg gtcaaggaat ccggcgccaa
     9301 agctggataa ggcatgaatc gtgattcgtc tctcaccacc ccgcccgttg cgcgtgcacc
     9361 cctcctctgt aaggagggga agttcttgtc ttgaaacccc ctccttgata tgggggggtg
     9421 gcgcgaagcg ccggggtggt ttgcaccggc cagcggtcgc aggttactcg gcaggagaaa
     9481 tgaaatgata cgactcgtag tagcgctcgt gtttgcagcc gcgacgccgc tgcaggccgc
     9541 tgacacctac ccttcgcgcc cggtgcgctt cgtggtgggc ttcctgccgg gcgggccgag
     9601 cgacaccatc gcccgcgtgc tcgccggcaa gctcggcgag gggctcgccc agaccgtcat
     9661 cgtggacaac cgcgccggcg ccggcggcaa catcagcgcc gagatcgtgg cgaaagcgca
     9721 gccggatggg cacacgctgc tgcttgggac cggcgggccg ctggtgatcg cgccgatcac
     9781 cggccagaag gt
//
LOCUS       DBNGWZ010000439         1958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3204209, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000439 DBNGWZ010000000
VERSION     DBNGWZ010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3204209"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1958
                     /locus_tag="AC16CX_13325"
     CDS             <1..>1958
                     /locus_tag="AC16CX_13325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3013527.1"
                     /translation="ILTALAQIVAEELDVSLERIRLAPADTTYSPNEGVTSGSQSITD
                     GGVALRYAAAEARDLILQRAAARLGASLEQLSVADGLVRARSGGSVTYWDLATDDLLA
                     REATAEIEPKAASLHTIIGTSAPRRDIPAKVSGAPIYVHDLDLPGMLHGRVARPPSYG
                     ARLASLDEAEVRALPGVVAVVRDGSFVGVVAEREEQALRALNRVKRIARWEESAVLPE
                     TTDPRFLFKEATEDQVISEKQDAAAAARAMKTLEAEYTRPYIAHAALGPSCSVARCDN
                     GRYTVWTHSQGIYPLRHDLAVVLGIEKERIAVTHMDGAGCYGHNGADDAALDAVLLAR
                     AVPGRAVRVQWMREDEFMWEPYGPAMVVKLKAALDPAGNIVSWNEDVWSNSHSTRPNG
                     LGGANLLAAWYLAEPFPPSPPRNPPLPGGGSHRNAIPIYEFPKERVTNRLVKRMPVRV
                     SALRSLGAMANVFAIESFMDELAAAAGADPVEFRLRHLKDPRARAVIETVAAKAGWHK
                     GEKGDGARGRGIGFARYKNGACYVAVIVEVEVGREVAVKRAWAAIDAGLVINPDGIAN
                     QTEGGIIQTVSWALKEAVRYDRSRILTSNWDDYPILNFEEAPQVEVTVVNRPDLPPLG
                     AGEGAQGPTAAAVANAIHNAMGVRLRDLP"
BASE COUNT          328 a          646 c          691 g          293 t
ORIGIN      
        1 atcctgaccg cgcttgccca gatcgtcgcc gaggagcttg atgtatcgct cgaacgtatc
       61 cggctcgcgc ctgccgatac cacctacagt cccaacgagg gcgtgacctc cggcagccag
      121 tcgatcacgg acggcggcgt cgcgctgcgc tatgcggcgg cagaggcgcg cgacctcata
      181 ttgcagcgcg cagccgcgcg gctgggcgcg tcgctcgagc agctttcggt ggctgacggc
      241 ctcgtcaggg cccgctccgg aggcagcgtc acttattggg atctcgccac cgatgacctg
      301 cttgcgcgcg aggccaccgc tgaaatcgaa cccaaagctg cttccctgca taccatcatc
      361 ggcacatccg ccccccggcg cgacatcccc gccaaggtga gcggcgcgcc gatttacgtc
      421 cacgacctgg atctgccggg catgttgcac gggcgcgttg cgcgtccccc gagttacgga
      481 gcgcgcctcg cgtcgttgga cgaagctgaa gtgcgtgcgc tgccgggcgt ggtggccgtg
      541 gtgcgcgacg gcagttttgt gggcgtcgtc gccgagcgcg aggaacaggc gctgcgagcc
      601 ctcaaccggg tgaagcgcat tgcacgctgg gaggagtccg ccgtgctgcc ggagacgacg
      661 gacccgcgct tcctgttcaa ggaggcgacc gaggatcagg tgatcagcga gaagcaagac
      721 gccgcagcgg cggctcgcgc catgaagacg ctcgaggcgg agtacacgcg gccctacatc
      781 gcgcatgccg ctctcgggcc gtcctgttcg gtggcgcgct gcgacaacgg ccgctacacc
      841 gtgtggacgc acagccaggg catctacccg ctgcgccacg acctcgcggt ggtgctgggc
      901 atcgagaagg agcgcattgc ggtgacccac atggacggcg ccgggtgtta cgggcataac
      961 ggagcggacg atgccgccct cgatgccgtc ctgctggcgc gcgccgtgcc cgggcgggcg
     1021 gtgcgggtgc aatggatgcg cgaagacgaa ttcatgtggg agccctacgg cccggcgatg
     1081 gtggtgaagc tcaaggcagc gctcgacccg gccggcaaca tcgtcagctg gaacgaggac
     1141 gtctggagca attcccacag cacgcgcccc aacggactgg gcggcgccaa cctgctggcg
     1201 gcatggtatc tcgccgaacc gttcccgccc tctccaccgc gcaacccgcc gctgcccggc
     1261 ggcggcagcc atcgtaacgc catcccgatc tatgaattcc ccaaggaacg cgtcaccaac
     1321 cgcctggtga agcgcatgcc ggtgcgggtg tccgcgctgc gctcgctggg cgcgatggcc
     1381 aacgtcttcg ccatcgaatc attcatggac gagctggccg ctgctgcggg cgccgatccg
     1441 gtcgagttcc ggctgcgcca tctcaaggat ccgcgcgcgc gggcggtgat cgaaacggtc
     1501 gcggcgaagg cggggtggca caaaggcgag aagggcgatg gcgcgcgcgg gcgcggcatc
     1561 gggttcgcgc gctacaagaa cggcgcctgc tacgtggcgg tgatcgtgga agtggaggtc
     1621 ggccgcgagg tcgcggtcaa gcgcgcctgg gcggcgatcg acgccggcct tgtgatcaat
     1681 ccggacggca tcgcgaacca gaccgaaggg ggcatcatcc agaccgtgag ctgggcgctc
     1741 aaggaagcgg tccgctatga ccgcagtcgc atcttgacga gcaactggga tgactatccg
     1801 atattgaatt tcgaggaagc gccgcaagtg gaagtgacgg tcgttaaccg gcccgatctg
     1861 ccgccgctcg gcgcgggcga gggggcacag ggaccgaccg ccgccgcggt cgccaatgcg
     1921 atccacaacg cgatgggcgt gaggttgcgc gacctgcc
//
LOCUS       DBNGWZ010000440         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3329056, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000440 DBNGWZ010000000
VERSION     DBNGWZ010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3329056"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="AC16CX_13330"
     CDS             <1..816
                     /locus_tag="AC16CX_13330"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013528.1"
                     /translation="AQQCSGISCWMRDEEHIEKAFDFGGMPARNGVAAAAMVSAGFTA
                     VEDVFSGERNFFVAYDETRRIGRSPEPQRLAQGLGTTYEILNTNIKRWSVGSPIQAPL
                     DSLLELIRAHGLRADDVERLTVRVAKQGANTTDNRAMPDICMQHMCAVMLMDGIVTFA
                     SSHDEKRMKDPKVLAIRSRVELIGDEELTQALPSRQGIVELRLKNGRELRHHTKAVRG
                     TAASPMTRPEVDEKAFHLMAPILGSARARKLCDAVWALERLSNMRNLRPLLRA"
     gene            865..2028
                     /locus_tag="AC16CX_13335"
     CDS             865..2028
                     /locus_tag="AC16CX_13335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3013529.1"
                     /translation="MNFELTPEQRAFRDSVRGFAEKHLREGALARAHNPDHPWDVARL
                     MARQGLLGITIPEQDGGQGGTLMDAIIAIETVAQVCPRSADVVQAGNFGAIRVLAEYG
                     TADQKSRYLKRLLSGDGVICVGMTEPEAGSAVTDLKTTAKPDGAGYRVSGEKVFTTHA
                     QYADVMVAYVRFGPGVGGIGSVLIERNTPGVKQGKPSRFMNGEEWVTTWFDNVYVPPE
                     NVVLKEGGFKKQMAGFNVERLGNTARSLALGRYCYETARQWALQRKQFGRLLAEFQGV
                     QWKFAEMKIKLDAGQLLLYRAAVNADRGLPSAEETAIAKAYCNQAGFEICSEAMQIMG
                     GMGYTEETLVEWCFRKCRGWMIAGGSIEVLKNRIAESVFERTFSQRPPKADKA"
     gene            2119..>2978
                     /locus_tag="AC16CX_13340"
     CDS             2119..>2978
                     /locus_tag="AC16CX_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR3013530.1"
                     /translation="MSDQKLAQALFAPRSVALIGASGDAAKNTSRPQRFLRKHGYNGR
                     ILPINRGRDQIFGEKAYADLRAVPEPVDHAFVMVPAAAVPEAIEQCCEKKVPVVTIFS
                     DGFAETGAAGREKQEALLKLARASGVRLLGPNCIGLLDLHSHLVLSVNAVLEKAEVTP
                     GATAIVSQSGSMMGGLMSRGLGRGAGFSKLVSVGNESDLSVGEITDMLVDDADTRVIL
                     LFMETIRDAGRLARAARRAFDADKPVIVYKLGRSEVGVELATSHTGAMTGSDDTAGAF
                     FRAHGMLRVE"
BASE COUNT          521 a          986 c         1007 g          464 t
ORIGIN      
        1 gcgcagcagt gctccgggat ctcgtgctgg atgcgcgacg aggagcacat cgagaaggcg
       61 ttcgacttcg gcggcatgcc ggcgcgcaac ggcgtcgcgg cggcggcgat ggtgtcggcg
      121 gggttcaccg cggtggagga cgtgttttcg ggcgagcgca acttcttcgt cgcctacgat
      181 gagacgcgcc gcatcggaag atcgcccgag ccgcagcgcc ttgcacaggg gctcggcacg
      241 acctacgaga tcctgaacac caacatcaag cgctggtcgg tgggctcgcc catccaggcg
      301 ccgctcgatt ccctgctcga gctcatccgc gcgcacgggc tgcgcgccga tgacgtggaa
      361 aggctcaccg tgcgcgtggc gaagcagggc gccaacacca ccgataaccg tgcgatgccc
      421 gacatctgca tgcagcacat gtgcgcggtg atgctgatgg atggcatcgt tactttcgcc
      481 tcctcgcacg acgagaagcg catgaaggac cccaaagtgc tcgcgataag aagccgcgtc
      541 gagctgatcg gcgacgagga actgacacag gcgctcccct cgcgccaggg catcgtggag
      601 ctgcggctga agaacggccg cgaactgcgc caccacacca aggcggtgcg cggcaccgcc
      661 gccagcccca tgacgcgccc ggaagtggat gagaaggctt tccacctgat ggcgccgatc
      721 ctgggaagcg cgcgcgcccg caagctgtgc gacgcggtgt gggcgctgga acgcctgtcc
      781 aatatgcgaa atctgcgtcc gctgttgagg gcatagcccc cgtgcggata gaatgacgat
      841 ccgcgtttcc cggccttcca tcatatgaac ttcgagttga cccccgagca gcgggcgttc
      901 cgcgactcgg tgcgcggctt cgccgagaag cacctgcgcg agggggcgct cgcgcgggcg
      961 cacaatcccg accacccgtg ggacgtcgcc cgcctgatgg cgcggcaggg gctgctgggc
     1021 atcaccatcc cggagcagga cggcggccag gggggcacgc tgatggacgc gatcatcgcg
     1081 atcgagaccg tcgcccaggt ttgcccgcgg agcgccgacg tggtgcaggc cggtaacttc
     1141 ggcgcgatcc gcgtgctcgc cgagtacggc accgccgacc agaagagccg ctatctcaag
     1201 cgcctgcttt caggcgatgg cgtgatatgc gtcggcatga ccgagcccga ggcaggctcc
     1261 gcggtgaccg atctgaagac caccgcgaaa cccgacggcg ccggctaccg cgtgagcggc
     1321 gagaaagtgt tcaccaccca cgcgcagtac gccgacgtga tggtcgccta cgtgcgcttc
     1381 ggaccgggcg tgggcggcat cggctcggtg ctgatcgaga ggaacacgcc cggcgtcaag
     1441 cagggcaagc cctcgcgctt catgaacggc gaggaatggg tcaccacctg gttcgacaac
     1501 gtctacgtgc cgcccgagaa cgtggtgctc aaggaaggcg gcttcaagaa gcagatggcc
     1561 ggcttcaacg tcgagcgcct cggcaacacc gcgcgctcgc tcgccctcgg gcgctactgc
     1621 tacgagaccg cgcgccagtg ggcgctgcag cgcaagcagt tcgggcgcct gctcgccgag
     1681 ttccagggcg tgcagtggaa gttcgccgag atgaagatca agctcgacgc cgggcagctc
     1741 ctgctctacc gcgccgcggt caacgccgac cgcgggctgc cctcggcgga agagaccgca
     1801 atcgccaagg cctactgcaa ccaggccggc ttcgagatct gctcggaagc gatgcaaatc
     1861 atgggcggca tgggctacac cgaggaaacg ctggtcgagt ggtgcttccg caaatgccgc
     1921 ggctggatga tcgccggcgg ctcgatcgag gttctcaaga accgcatcgc cgaatccgtc
     1981 ttcgagagga cgttctcgca gcgtccgccg aaggcggata aagcgtaagc tattcaagtt
     2041 cattgcgttc atcattctcc ggtccctttc gataccgcgc ttaacgcggc gcttccagtt
     2101 ttggcagata caagcacgat gtccgaccag aagctcgctc aagcactgtt cgcgccgcgt
     2161 tcggtggcgc tgatcggcgc ctccggcgac gcggccaaga acacttcgcg cccgcagcgc
     2221 ttcctgcgca agcacggcta caacggacgc atcctgccga tcaaccgcgg ccgtgaccag
     2281 atcttcggcg agaaggccta tgccgacctg cgcgccgtgc cggaaccggt ggaccacgcg
     2341 ttcgtcatgg tgccggccgc ggccgtgccc gaggcgatcg agcagtgctg cgagaagaag
     2401 gtgccggtcg tcaccatctt cagcgacggc ttcgccgaaa ccggcgcggc cggtcgcgag
     2461 aaacaggagg cgctgctcaa gctcgcgcgg gcgagcggcg tgcggctgct cgggcccaac
     2521 tgcatcgggc tgctcgacct gcattcgcat ctcgtgctct cggtcaacgc agtgctcgag
     2581 aaggcggagg tcactcccgg cgccactgcg atcgtctcgc agagcggaag catgatgggc
     2641 gggctcatgt cgcgcggcct cggccgcggc gcgggcttct ccaagctggt gtcggtcggc
     2701 aacgagtccg acctttcggt gggcgagatc accgacatgc tggtggatga cgccgacacg
     2761 cgggtgatcc tgctgttcat ggagacgata cgcgatgcgg gtcgcctcgc gcgcgccgcg
     2821 cgccgcgcct tcgacgccga caagcccgtt atcgtctaca agctcggccg ctccgaggtc
     2881 ggtgtcgagc tcgccacctc gcacaccggc gcgatgaccg gctccgacga cacggcgggc
     2941 gctttttttc gcgcccatgg catgctgcgg gtggagat
//
LOCUS       DBNGWZ010000441         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_562103, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000441 DBNGWZ010000000
VERSION     DBNGWZ010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_562103"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2192
                     /locus_tag="AC16CX_13345"
     CDS             <1..>2192
                     /locus_tag="AC16CX_13345"
                     /inference="COORDINATES: protein motif:HMM:NF024767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013531.1"
                     /translation="GRIRLLSLTGTRTLEYSPEFRWQQTNNLTRYQFRLLDDMGKPLH
                     EAEVEGVAYRLPPSVKLNESVAYTWELSAQFADGRRYTSTGDFSLASSELHAQVEALR
                     PAAGVPFAERVMFAAWLEQAHLRDEARKHWQALLAERPDEPALLALAGETVHPPRTAA
                     DIAALLDQYRPDPEKVAAFKATLDREPPAGANRDALLRFYIERAQAAQNLGMPARQLA
                     DLRRALEFVKGTEDEWTVMQDIFITEIQSGNFATALKIKEQMPRLVGRLRGRLPADYG
                     NAAFMYTLIGDVPAARSALRNLEGVMWDLNRSRNWAQFGDAWTAHLERTRAEVLNAEG
                     KYVEAEGFYRKALAAAERNLEIRLRDTSDPLVPFERYYNWVESNEQRLANNLILQGRL
                     AEAELSVRNVLQRQLSRLGRYAPTTAGVVGQFSRLLNEQGRFRDAEAMARAAIEIYQA
                     IGTAPAASGYIGARRQLGRALVAQLRWKEALPVFEAMGADIAQDPVLIRMGRGDPNWG
                     LALVKTGQPDKALPMLEGGLKRTIERLGAAHYEVAERRGFLAMALAESGQRQRAFGEF
                     QEAVKVLLARDRAEGDDEGGASARAWRLALILEGYIKLLYEIRNELGGQRAGFDPAAE
                     AFRVADAARGQSTQRALTASAARAAAGDPALADLVRREQDAKQQVSVLYNTLLRLLNA
                     PSDQQLPQVAAQMRARIQELEKERRTLLAELEKRFPAYADLVNPRPAT"
BASE COUNT          394 a          690 c          775 g          333 t
ORIGIN      
        1 cggggcgcat ccggctgctc tcgctcaccg ggacgcggac gctcgaatac tcgcccgaat
       61 tccgctggca gcaaaccaat aacctgacgc gctatcaatt caggcttctg gacgacatgg
      121 gcaagccgct gcacgaggcc gaggtcgaag gcgtggcgta ccggctgccg ccctcggtca
      181 aactcaatga aagcgtggcc tatacgtggg agctgtcggc gcagtttgcc gatggcaggc
      241 gctacaccag cacgggtgat ttcagcctgg catccagtga gctgcacgcc caggtcgaag
      301 cgctgcgtcc ggcggccggg gtgccgtttg ccgagcgcgt catgtttgca gcgtggctgg
      361 agcaggcgca cctgagggac gaggcgagga agcactggca ggcgctgctc gccgagcggc
      421 cggacgagcc ggcactcctg gcgctcgcgg gagaaacagt ccatcccccg cgcaccgccg
      481 ccgatatcgc ggcgttgctc gaccagtacc ggcccgatcc cgagaaagtg gcagcgttca
      541 aggcgacgct cgaccgggag cctccggctg gggcgaatcg ggacgcgttg ctgcgctttt
      601 acatcgaacg cgctcaggcc gcgcagaatc tcggaatgcc ggcgcgccag ctcgccgatc
      661 ttcgccgcgc gctcgagttc gtgaagggca cggaggacga gtggaccgtc atgcaggata
      721 tcttcattac cgagatccag tcgggcaatt tcgccactgc cctgaagatc aaggaacaga
      781 tgccgcggct ggttggcaga ctgcgcggcc ggctgccggc ggactacggc aatgctgcgt
      841 ttatgtatac gctgattggt gatgtaccgg ctgcgcgcag cgctctccgc aacctcgagg
      901 gagtcatgtg ggatctcaac cgcagccgta actgggcgca gttcggcgat gcgtggaccg
      961 cacatctcga acgcacgcgc gcggaagtgc tgaacgccga gggcaagtac gtggaagccg
     1021 aaggcttcta tcgcaaggcg ttggctgccg ccgaacgcaa tctcgagatc cggctgcgag
     1081 acacgagcga tcccctggtt cccttcgagc ggtactacaa ttgggtggaa tccaacgagc
     1141 agcgactcgc gaacaacctg atcctgcaag ggcggctggc cgaggcggag ctctcggtgc
     1201 gcaatgtgct gcagcgccaa ttgagccgcc tcgggcgtta cgcgcccacg accgcggggg
     1261 tggtcgggca gttttcacgg ctcctcaacg agcaggggcg tttccgcgac gcggaggcga
     1321 tggcgcgcgc cgctatcgag atctaccagg cgatcggcac ggcgccggcg gcttccggtt
     1381 acatcggcgc gcgccggcag ctcggcaggg cgctggtggc gcaattgcgc tggaaagaag
     1441 cgctgccggt gttcgaggcg atgggcgcgg acatcgcgca ggatccggtg ctcatcagaa
     1501 tgggtcgcgg tgacccgaat tgggggctcg cgctggtcaa gaccggccag ccggacaagg
     1561 cgctcccgat gctggaagga gggctcaagc gcacgatcga acgactgggc gcggcgcact
     1621 acgaggttgc cgaacggcgc ggatttctcg caatggcgct cgccgaaagc gggcagcggc
     1681 agcgggcgtt tggcgagttc caggaagccg tgaaagtgct gctggcgcgc gaccgtgcgg
     1741 aaggcgatga cgaaggcggc gcctcggcgc gggcctggcg cctcgccctg atactcgaag
     1801 gctacatcaa gctgctctac gaaatccgca atgaactcgg ggggcagcgt gccggcttcg
     1861 atccggcggc ggaagcgttc cgagtcgcgg atgccgcgcg cggacaaagc acacaacggg
     1921 ctctcaccgc ttccgcggcg cgcgcggcgg cgggcgatcc ggcgctcgcc gacctggtcc
     1981 gcagggaaca ggacgcgaag cagcaggtat cggtgctgta taacacgctg ctgcggctcc
     2041 tcaacgcgcc gtcggaccag cagctgccgc aagtcgccgc gcagatgcgc gcccgcatcc
     2101 aggaactgga gaaggagcgg cggacgctct tggcagaact ggagaagcgc ttcccggctt
     2161 acgccgatct ggtcaacccg cggccggcga cg
//
LOCUS       DBNGWZ010000442         5404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2954638, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000442 DBNGWZ010000000
VERSION     DBNGWZ010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2954638"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..258)
                     /gene="rpmA"
                     /locus_tag="AC16CX_13350"
     CDS             complement(1..258)
                     /gene="rpmA"
                     /locus_tag="AC16CX_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR3013532.1"
                     /translation="MAHKKAGGSSRNGRDSQSKRLGVKRYGGERVSAGAIIVRQRGTQ
                     VHPGANVGMGRDHTLFAKVTGHVQFAQKGPDGNKTVSVVPA"
     gene            complement(353..661)
                     /gene="rplU"
                     /locus_tag="AC16CX_13355"
     CDS             complement(353..661)
                     /gene="rplU"
                     /locus_tag="AC16CX_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014110913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR3013533.1"
                     /translation="MYAVVRTGGKQYRVSAGEKLRIEQIPAEVGQEILLEEVLLVADG
                     DALKVGAPLVPGAKVKVRVLKHGRGEKVRIFKLKRRKHYRKSQGHRQNYTEIEILGIA
                     "
     gene            847..1815
                     /gene="ispB"
                     /locus_tag="AC16CX_13360"
     CDS             847..1815
                     /gene="ispB"
                     /locus_tag="AC16CX_13360"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HIR3013534.1"
                     /translation="MTLEAIRGFMEADMRAVDNVIRDRLDSDVLLIRQVAEYIVGGGG
                     KRLRPMLLLLSAGAFGYRGPHRHELAAVIEFIHTATLLHDDVVDESDLRRGRATANAS
                     FGNAAAILVGDFVYSRAFQMMVEVDSMRVLQVLADATNVIAEGEVMQLMSCHNPDIEE
                     NGYLEVIRCKTAKLFEAATRLGAILAGAPRGEEDAVADYGTHLGTAFQLIDDVLDYSG
                     EQAMIGKNVGDDLAEGKPTLPLIYSIKHGTPKQAQLVRHAVEHGGRDELAAVIEAIRS
                     TGALDYAREQARRESRAACDALARLPHSRHREYLLQLADFAVTRTF"
     gene            1929..2005
                     /locus_tag="AC16CX_13365"
     tRNA            1929..2005
                     /locus_tag="AC16CX_13365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1963..1965,aa:Pro,seq:cgg)
     gene            2066..2305
                     /locus_tag="AC16CX_13370"
     CDS             2066..2305
                     /locus_tag="AC16CX_13370"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HIR3013535.1"
                     /translation="MAKIILLVLGLLLVYWILKSYRRRVDSGESRTPPAGGEDMVQCA
                     QCGVHLPRSESITTQGRFFCSAEHQRTHQSGQRSG"
     gene            2302..3918
                     /locus_tag="AC16CX_13375"
     CDS             2302..3918
                     /locus_tag="AC16CX_13375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013536.1"
                     /translation="MSPVGTQRPALWEADAAAPAGEQLEPFWRSLFYFNVYRLLVTLL
                     LLMSVAIWGANLWFGSRDLTLFIVADVAYLVFGVACFVLISARWRFNLQLTLQVAADV
                     GFIVLLLYASNGISSGLGLLLLTTLAGAGLISRGRLTLFFAALASIGVLLEHTYEVLK
                     LDAPETQFVQAGLLSAAYFAIAWLAHALARYTVASEQLAAQREIDLANMEQVNQLVIR
                     DMQDGVLVVDERGAIRQCNPRAERLLGPLPRGHGEATLAEYAPALAARFEAWRARAGV
                     DTSPHTPFVNNVNAHFVPIGGSRQVGAVIFLEDLSRIQAEARQLKLAALGRLTANIAH
                     EVRNPLGAISHAAQLLQEEPGVGDTAARLITIINENSHRLDRMVNDVLRLNRGERAHC
                     ERFKLADFLKGFVEQFSQTGKIDPGIFRIELMADPEILFDRSHLNQVMWNLCRNALRH
                     CRHGPASIVIRVSAERAGSIVKLDVMDDGPGVAPETRAQLFEPFFTTATGGTGLGLYI
                     AREVCEANGATLDHVETLRGAQFTVQCWAG"
     gene            4020..5402
                     /locus_tag="AC16CX_13380"
     CDS             4020..5402
                     /locus_tag="AC16CX_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR3013537.1"
                     /translation="MSAKSHILVVDDEADIRELLGMTLARMGLEAHCASSTSEAYALL
                     AKNSYDLCLTDMRLPDGDGLTVVDYVAKHHPNLPVAVITAHGSTENAVAALKAGAFDY
                     LAKPVSLDQLRTLVRSALKLPRAAQRRAQEEEAPVPAGASLLIGGSGPMQAVRAMIDR
                     LARTQAPIHVSGESGSGKELAARLIHVKGSRADGPFIPVNCGAIPENLMESEFFGYKK
                     GAFTGAAADRDGFFQAASGGTLFLDEVAELPLPMQVKLLRAIQEKKVRKVGSTGEESV
                     DVRLISATHRNLGEEVKNGKFRQDLYYRLNVIELKMPALRAMREDIPLLAAAILRRQG
                     DTSGGKASRLSAAALERLGTYDFPGNVRELENVLERALALTTGGEIRAEDLQLAPTRL
                     AQEAAPAALSGLPLQERLDAVERQAILAALEQTRYNRTAAAKLLGITFRALRYRMERL
                     GIKEELDSKG"
BASE COUNT          890 a         1822 c         1766 g          926 t
ORIGIN      
        1 tcaggcggga acgacgctca ccgtcttgtt gccgtccggc cccttctgcg cgaactgcac
       61 atgccccgtc accttggcga acagcgtatg gtcgcgcccc atgccgacgt ttgcgccggg
      121 gtgcacctgg gttccgcgct ggcgcacgat gattgcgccc gcgctcaccc gctcgccgcc
      181 gtagcgcttc acgccgaggc gcttcgactg cgagtcccgg ccgttacgcg agctgccgcc
      241 tgccttcttg tgtgccattg agcctgtctc ctgtctctat caggctgttt cgtggccacc
      301 gaaacagccc ctgatgtggg gagcacgagg ggagtatccc ttcgttcccg acctaggcga
      361 tgccgaggat ctcgatctcg gtgtagttct ggcggtgccc ctggctcttg cggtagtgct
      421 tgcggcgctt gagcttgaag atgcgcacct tctcgccgcg gccgtgcttg agcaccctca
      481 ccttgacctt ggccccgggc acgaggggcg cgcccacctt gagcgcgtca ccgtcggcca
      541 ccagcagcac ctcctcgagc aggatttcct gcccgacctc agcgggaatc tgctcgatcc
      601 tgagtttctc gccggcactg acccgatact gcttgccgcc ggtcctgacc accgcgtaca
      661 tgccgccgcc cgttgaacct ggattggaaa gccgcgcatt ctacaaaaag cacgcccgca
      721 agtcaaaaaa acgcgccggc ggatctccgg gcggcgtttg acatcaaacg gttggcaggc
      781 ccggacgcgc aagcccggcg cggttttttt actgtatcat ccaccctctt ttcacgaggc
      841 gcagcggtga cactggaggc gatacgcggg ttcatggagg cggacatgcg cgccgtggac
      901 aatgtgatcc gcgaccgcct ggattccgac gtgctgctga tccgccaggt ggcggaatac
      961 atcgtcggcg gcgggggcaa gcgcctgcgc ccgatgctgc tgctgctctc cgccggcgcc
     1021 ttcggttacc ggggaccgca ccgccacgag ctcgccgcgg tgatcgagtt catccatacc
     1081 gcgacgctgc tgcacgacga tgtcgtggac gaatccgatc tgcggcgcgg gcgtgccacg
     1141 gccaacgcgt cgttcggcaa cgccgccgcc atcctggtcg gcgatttcgt ctattcgcgc
     1201 gcgttccaga tgatggtgga agtggacagc atgcgcgtgc tgcaggtgct ggcggacgcg
     1261 accaacgtca tcgccgaagg cgaggtgatg cagctcatga gctgccacaa tcccgacatc
     1321 gaggaaaacg gctacctcga ggtgatccgc tgcaagacgg cgaagctgtt cgaggccgcc
     1381 acgcggctcg gcgcgatcct ggccggggcg ccgcgtggcg aagaggacgc ggtcgccgac
     1441 tacggcacgc acctcggtac cgcgtttcag ctgatcgacg acgtgctcga ctactcgggc
     1501 gagcaggcga tgatcggcaa gaacgtgggc gacgatctcg cggagggcaa gcctaccctg
     1561 ccgctcattt actccatcaa gcacggcacg ccaaaacagg cgcagctcgt gcggcacgcc
     1621 gtcgagcacg gcgggcgcga cgagctcgcc gcggtgatcg aggcgatccg ttccaccggc
     1681 gcgctcgatt acgcgcgcga gcaggcgcgc cgggaatcgc gcgcggcgtg cgacgcgctc
     1741 gcgcgcctgc cgcattcaag gcaccgcgaa tatttgctac aattggcgga ctttgcggtg
     1801 acgcggactt tttaaactgt cagcacccag ctgtcagctt tcagctaaac catgcgcggc
     1861 catgccgcgt gtttttttcc gcaggctgat ggctgacggc tgaccgccga tagctgattg
     1921 cttgcagtcg gggagtagct cagcctggta gagtactgcg ttcgggacgc aggtgtcgga
     1981 ggttcaaatc ctctctcccc gaccactttt ttccgggaac ggtgaccggc aaacggccac
     2041 acctgaaccg ccggcggagc ctctaatggc aaagatcatt cttctcgtcc tcgggcttct
     2101 gctcgtctac tggatcctga agagctaccg ccgccgcgtt gacagcggcg agtcgcggac
     2161 gccccccgcg ggcggggaag acatggttca atgcgcccag tgcggcgtgc acctgccgcg
     2221 tagcgaaagc atcaccacgc agggcaggtt cttctgctcc gccgagcacc agcggacgca
     2281 ccagagcggg cagcgttccg gatgagcccg gtcggcaccc agcggcctgc gctgtgggag
     2341 gcagacgccg ccgcgcccgc aggcgagcag ctcgagccgt tctggcgctc gctgttctat
     2401 ttcaacgttt accgcctgct cgtcacgttg ctgctgctca tgagcgtggc gatctggggg
     2461 gccaacctct ggttcggctc gcgcgatctg acgctgttca tcgtcgccga tgtcgcctac
     2521 ctggtgttcg gcgtcgcgtg cttcgtgctg atcagcgcgc gctggcgctt caacctgcaa
     2581 ctgacgctcc aggtggcggc cgacgtcggc ttcatcgtgc tcctgctcta cgcgagcaac
     2641 ggcatctcga gcggactggg cctgttgctg cttaccacgc ttgccggcgc ggggctgatc
     2701 agccgcggcc ggctgacgct ctttttcgcc gcgctcgcca gcatcggtgt gctgctcgag
     2761 catacctacg aggtgttgaa gctcgacgct cccgagaccc agttcgtgca ggccggtttg
     2821 ctgtcggcgg cctatttcgc gatcgcctgg ctcgcccacg ccctcgcgcg ctataccgtg
     2881 gcaagcgagc agctcgccgc gcagcgcgag atcgacctcg ccaacatgga gcaggtgaat
     2941 cagctcgtga ttcgcgacat gcaggacggc gtgctggtgg tggacgaacg cggggcgatc
     3001 cgccagtgca acccccgggc cgagcgcctg ctggggccgc tgccgcgcgg gcacggcgaa
     3061 gccacgctcg ccgaatacgc gcccgcactc gccgcgcgat tcgaggcgtg gcgcgcgcgc
     3121 gcgggcgtgg ataccagccc gcacacgccg ttcgtaaaca acgtcaacgc acacttcgtg
     3181 ccgatcggcg gcagccggca ggtcggcgcg gtcatctttc tcgaggacct gagccgcatc
     3241 caggccgagg cgcgccagtt gaagctcgcc gcgctgggac gcctcaccgc caacatcgcg
     3301 catgaggtca ggaaccccct gggcgcgatc agccacgccg cgcagctgct gcaggaggag
     3361 ccgggcgtgg gcgacaccgc ggcgcgcctc atcaccatca tcaacgagaa cagccaccgc
     3421 ctcgaccgca tggtgaacga cgtgctgcgg ttgaaccgcg gcgagcgcgc gcactgcgag
     3481 cgtttcaagc tcgccgattt cctgaaggga ttcgtcgagc agttctcgca gactgggaaa
     3541 atcgatcccg gcatcttccg catcgagctc atggcggacc ccgagatcct gttcgaccgc
     3601 agtcacctca accaggtgat gtggaacctc tgccgcaacg cgctgcgcca ttgccggcac
     3661 ggccctgcga gcatcgtgat ccgcgtctcg gcggagcgcg cgggcagtat tgtaaaattg
     3721 gacgtgatgg acgacgggcc gggtgtcgcc cccgagacgc gggcacagtt gttcgagccg
     3781 ttcttcacca ccgccaccgg cggcaccggt ctcggtctct acatcgcgcg tgaggtatgc
     3841 gaggcgaacg gcgccacgct cgatcacgtc gagaccctgc gcggcgcgca gttcaccgtg
     3901 caatgctggg ccggctgacg tgcgagtgat taggtgatta agtgactgag tgattaagtt
     3961 gttgcgcact cggcgaggca agatgcttaa tcacttaatc actcaatcac tgggtttgaa
     4021 tgagtgcgaa atcccacatc ctcgtcgttg acgacgaagc ggatatccgc gagctgctcg
     4081 gcatgacgct cgcgcgcatg gggctcgagg cgcactgcgc ctcgagcact tccgaagcct
     4141 atgccctgct ggccaagaac agctacgact tgtgcctcac cgacatgcgg ctgcccgacg
     4201 gcgacgggct cacggtggtg gactacgtcg ccaagcacca tcccaacctg ccggtggcgg
     4261 tgatcaccgc tcacggcagc accgagaacg cggtggccgc gctcaaggcc ggcgcgttcg
     4321 actatctcgc caaacccgtc tcgctcgacc agttgcggac gctggtgcgc tccgcgctca
     4381 aactgccgcg cgcagcccag cggcgggcgc aggaggaaga ggcgcccgtg ccggcgggtg
     4441 catcgctgct gatcggggga tccggcccga tgcaggccgt gcgcgcgatg atcgaccgcc
     4501 tcgcgcgcac ccaggcgccg atccacgtca gcggcgaatc cggcagcggc aaggagctcg
     4561 cggcgcgtct gatccatgtc aaaggctcgc gggccgacgg tccgttcatc ccggtcaact
     4621 gcggcgcgat cccggagaac ttgatggaga gcgagttctt cggctacaag aagggcgctt
     4681 tcaccggcgc cgcagccgat cgcgacgggt tcttccaggc cgcgagcggc gggacgctgt
     4741 ttctcgacga ggtggcggag cttccgctgc cgatgcaggt gaagctgctg cgcgcgatcc
     4801 aggagaagaa agtgcgcaag gtcggatcca ccggcgagga gtccgtggac gtgcgtctca
     4861 tcagcgccac ccaccgcaat ctcggggagg aagtcaagaa cggcaagttc cggcaggacc
     4921 tctattaccg gctgaacgtg atcgagctca agatgccggc gttgcgcgcc atgcgcgagg
     4981 acatcccgct gctcgccgcg gccatcctca ggcggcaggg tgacacgagc ggcggcaagg
     5041 cgtcgcggtt gtcggcggcc gcactcgaac ggctcgggac ctatgacttt cccggcaacg
     5101 tgcgcgagct cgagaacgtg ctcgagcgcg cgctcgcgct gaccaccggg ggcgagatcc
     5161 gcgccgagga tctccagctc gcgccgaccc gccttgcgca ggaggcggcg ccggccgctc
     5221 tgtccggtct gcccctgcag gaacggctcg atgcggtcga acgccaggcc atcctcgccg
     5281 cgctcgagca aacgcgctac aaccgcaccg cggcggcgaa gctgctcggc atcaccttcc
     5341 gcgcgctgcg ctatcgcatg gagcggctcg ggatcaagga agagctcgac tccaaaggct
     5401 gacc
//
LOCUS       DBNGWZ010000443        11809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_183306, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000443 DBNGWZ010000000
VERSION     DBNGWZ010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11809
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_183306"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /gene="tuf"
                     /locus_tag="AC16CX_13385"
     CDS             <1..1106
                     /gene="tuf"
                     /locus_tag="AC16CX_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HIR3013538.1"
                     /translation="AITRVLGKKDPKIQFRSFDSIDNAPEEKARGITIAVAHVEYSTA
                     KRHYAHVDCPGHADYIKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYI
                     VVFMNKADMVDDKELLELVEVEVRELLSKYDFPGDKTPIVIGSALKAMEGDKGELGEG
                     AIMKLAEALDAYIPTPKRVIDKPFLMPVEDVFSISGRGTVVTGRVERGVVKVGDEIEI
                     VGLRPTQKTVCTGVEMFRKLLDEGQAGDNVGILLRGTKREEVERGQVLAKPATITPHT
                     KFTAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGSIELAQGAEMVMPGDNVSITV
                     SLIQPIAMEEGLRFAIREGGKTVGAGVVAKVIE"
     gene            1193..1510
                     /gene="rpsJ"
                     /locus_tag="AC16CX_13390"
     CDS             1193..1510
                     /gene="rpsJ"
                     /locus_tag="AC16CX_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR3013539.1"
                     /translation="MATVKSQKIRIRLKAFDYRLIDQSALEIVDTAKRTGAVVKGPVP
                     LPTRKQRFDLLRSPHVDKKSREQLEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVE
                     IKL"
     gene            1569..2252
                     /gene="rplC"
                     /locus_tag="AC16CX_13395"
     CDS             1569..2252
                     /gene="rplC"
                     /locus_tag="AC16CX_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR3013540.1"
                     /translation="MSLGLVGRKVGMTRVFTDEGDAVPVTVVDVSNNRVSQIKTAETD
                     GYAAVQVAFGRRRPARVNRAAAGHFAKAGVEAGHTLREFRVKPDEVANLKVGGAIGAD
                     LFKIGQKVDVSGTTQGKGFTGVIKRHHFSSNRASHGNSVSHNKPGSIGQNQDPGRVFP
                     GKRMAGHMGAARRTVQNVEIIRIDTERQLLLIKGALPGSRGGGVTVKPTTRAARAAAP
                     AKPAAKPAK"
     gene            2262..2882
                     /gene="rplD"
                     /locus_tag="AC16CX_13400"
     CDS             2262..2882
                     /gene="rplD"
                     /locus_tag="AC16CX_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR3013541.1"
                     /translation="MELPVINEQGKPTATVAASDAAFAREYNESLVHQVVTAYLANAR
                     QGTRAQKARGEINKSNKKPWRQKGTGRARAGRASSPLWRGGGKIFPNLPDENFSQKLN
                     RKMYRAGVSSILSQLAREERLAVVEKFTVDAPKTKLLMAKLKGMGLDDVLIITDSLDE
                     NLRLSSRNLPNVDVVTVGLADPLSLMRHAKVLVTKGAVAKIGELFA"
     gene            2879..3208
                     /gene="rplW"
                     /locus_tag="AC16CX_13405"
     CDS             2879..3208
                     /gene="rplW"
                     /locus_tag="AC16CX_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR3013542.1"
                     /translation="MTTATPNIQQQARLMQIVLAPVISEKSTFVADKHNQVIFRVASG
                     ATKPEIKAAVEFMFKVQVQSVTVANVRGKSKRFGQYTGRRNHWRKAYVRLKPGQEINF
                     AEQAEAK"
     gene            3208..4044
                     /gene="rplB"
                     /locus_tag="AC16CX_13410"
     CDS             3208..4044
                     /gene="rplB"
                     /locus_tag="AC16CX_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR3013543.1"
                     /translation="MLVKIRPVTPGQRQQVKVVNPELHKGGPVPHLVERKIRKGGRNM
                     HGEITMRHQGGGHKQHYRKVDFKRDKDAITARVERIEYDPNRSAFLALLCYADGERRY
                     IIAPKGVSAGAQLVSGPDAPIKAGNTLPLRNIPVGTTVHCVEMLPGKGAQLARAAGAS
                     VQLLAREGDYAQVRLRSGEIRKVHINCRATIGEVGNEEHSLESIGKAGRVRWRGIRPT
                     VRGVAMNPVDHPHGGGEGKKAAARDPVSPWAVLTKGYKTRKNKRTDAMIIRRRNAKTV
                     GG"
     gene            4048..4299
                     /gene="rpsS"
                     /locus_tag="AC16CX_13415"
                     /pseudo
     CDS             4048..4299
                     /gene="rpsS"
                     /locus_tag="AC16CX_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            4444..4782
                     /gene="rplV"
                     /locus_tag="AC16CX_13420"
     CDS             4444..4782
                     /gene="rplV"
                     /locus_tag="AC16CX_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR3013544.1"
                     /translation="MNTTAKLRGVRLSAQKGRLVADLIRGKPVEQALNILQFAPKKGA
                     VIIRKVLESAIANAEHNDGADIDELKITNILVERGTFLRRFRAQSKGRGARINKPTCN
                     IFVTVGDGRK"
     gene            4785..5603
                     /gene="rpsC"
                     /locus_tag="AC16CX_13425"
     CDS             4785..5603
                     /gene="rpsC"
                     /locus_tag="AC16CX_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR3013545.1"
                     /translation="MGQKINPIGFRLAVNRNWASRWYANNKNFSSMLLEDIRVRDYLK
                     KKLAHAAVSRVVIERPAKNARITVHSARPGVVIGKKGEDIELLKGELQKMLGVPVHVN
                     IEEVRKPETDAQLIADSIAQQLQKRIMFRRAMKRAITNAMRLGAQGIKIASAGRLNGI
                     EIARYEWYREGRVPLHTLRAEIDYGVSEAKTSYGVIGIKVWVFKGEVLAKSDQPAAAI
                     AAALAAPAAAPAAVDKPAPAAAPGGAAKDKAKKPRAPRKSPAKSGAKHAATRKT"
     gene            5581..5997
                     /gene="rplP"
                     /locus_tag="AC16CX_13430"
     CDS             5581..5997
                     /gene="rplP"
                     /locus_tag="AC16CX_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011901907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR3013546.1"
                     /translation="MLQPARRKYRKEQKGRNRGIATRGNKVSFGEYGLKATTRGRLTA
                     RQIEAARRAMSRHVKRGGRIWIRIFPDKPVSQKPAEVRMGNGKGNPEFWVSEIKPGAV
                     LYEMDGVNEQEAREALRLAASKLPLRTTFVSRLAGG"
     gene            6001..6198
                     /gene="rpmC"
                     /locus_tag="AC16CX_13435"
     CDS             6001..6198
                     /gene="rpmC"
                     /locus_tag="AC16CX_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR3013547.1"
                     /translation="MKAAELKAKSPDELRQELNALLKAQFSLRMQKATQQLTNTSQLG
                     KVRRDIARVRTALAGKGQGKA"
     gene            6195..6458
                     /gene="rpsQ"
                     /locus_tag="AC16CX_13440"
     CDS             6195..6458
                     /gene="rpsQ"
                     /locus_tag="AC16CX_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3013548.1"
                     /translation="MSEAANKRQLTGRVVSDKMAKTVTVLVERRMKHPLYGKFVTRSR
                     KYHAHDEANEFKEGDLVLIEECRPISRTKAWRVIRLVEKAKIV"
     gene            6658..7026
                     /gene="rplN"
                     /locus_tag="AC16CX_13445"
     CDS             6658..7026
                     /gene="rplN"
                     /locus_tag="AC16CX_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3013549.1"
                     /translation="MIQMQSVLDVADNTGARSVMCIKVLGGSKRRYAGIGDVIKVSVK
                     DAAPRGRVKKGEIYDAVVVRTAKGVRRNDGSVIKFDSNAAVLLTPKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            7038..7355
                     /gene="rplX"
                     /locus_tag="AC16CX_13450"
     CDS             7038..7355
                     /gene="rplX"
                     /locus_tag="AC16CX_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3013550.1"
                     /translation="MRKIRKGDDVVVLAGRDKGKRGTVLRIVDAEHVLVEGANRVKKH
                     QRPIPMKGITAGIIDKEMPLHVSNVALYNPGTKKGDRVGIRVLEDGRRVRYFKSNGEV
                     IDA"
     gene            7577..8116
                     /gene="rplE"
                     /locus_tag="AC16CX_13455"
     CDS             7577..8116
                     /gene="rplE"
                     /locus_tag="AC16CX_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014112130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3013551.1"
                     /translation="MPRLQIHYRDAVVKQLMQQFGYKTAMQVPRIEKIVLNMGVSEAV
                     ADKKIMDNAVADMAKIAGQKPVVTKSRKSIAVFKIRSGYPIGCKVTLRGRRMYEFLDR
                     LVNIAMPRIRDFRGISGRAFDGQGNYNMGVKEQIIFPEIEYDKIDAIRGMNITITTTA
                     KSDNEAKALLSAFRFPFRN"
     gene            8137..8442
                     /gene="rpsN"
                     /locus_tag="AC16CX_13460"
     CDS             8137..8442
                     /gene="rpsN"
                     /locus_tag="AC16CX_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR3013552.1"
                     /translation="MAKTAVINRDKKRRETVKKYAARRKELLEIVNNQKLSPEERYAG
                     REKLQKLPRDASPVRLRNRCALTGRPRGVYRKFGLARGKLRLIALHGEIPGVIKASW"
     gene            8455..8850
                     /gene="rpsH"
                     /locus_tag="AC16CX_13465"
     CDS             8455..8850
                     /gene="rpsH"
                     /locus_tag="AC16CX_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3013553.1"
                     /translation="MAMTDPIADMLTRIRNAQCAEKQSVAIPASKVKTAIAQVLKDEG
                     YVEDFAVRELDGRPTLEINLKYYAGKPVIEKIERVSRPGLRIYRPKDELPKVMNGLGV
                     AIVSTSRGVMTDRKARGLGVGGEVLCIVA"
     gene            8863..9396
                     /gene="rplF"
                     /locus_tag="AC16CX_13470"
     CDS             8863..9396
                     /gene="rplF"
                     /locus_tag="AC16CX_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3013554.1"
                     /translation="MSRIGNAPVTLPDKVEVAITPGEISVKGPLGALSRKLSPEVKVE
                     KVENRIQFKPAGNSRLANAMSGTLRALVANMVTGVTKGFERRLTLVGVGYRAQAQGDK
                     LNLTLGFSHPVVYPMPKGIKVETPTQTEIVIKGMDRQLVGQVAAEVRAWRSPEPYKGK
                     GVRYTGEEVVIKETKKK"
     gene            9408..9764
                     /gene="rplR"
                     /locus_tag="AC16CX_13475"
     CDS             9408..9764
                     /gene="rplR"
                     /locus_tag="AC16CX_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3013555.1"
                     /translation="MFDRNRQRLRRARQTRSKIAELAAVRLTVHRSNSHIYAQVIDPS
                     GAKVLAAASTIEPEVRGAVKYGGNVKAAAEVGRRIAEKAKKLGIEKVAFDRAGYRFHG
                     RVKALADAAREAGLKF"
     gene            9785..10309
                     /gene="rpsE"
                     /locus_tag="AC16CX_13480"
     CDS             9785..10309
                     /gene="rpsE"
                     /locus_tag="AC16CX_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR3013556.1"
                     /translation="MAKLQASKMQSGEDRGDGLREKMVAVNRVTKVVKGGRVLGFAAL
                     AVVGDGDGGIGMGKGKAREVPVAVQKAMEEARRKLVKVSLKHGTLQHAVIGHHGSSRV
                     YMQPASDGTGIIAGGPMRAVFEVMGVHNVLAKCLGSTNPYNVVRATINGLTSMNTPAQ
                     IAAKRGKSVEDITG"
     gene            10312..10500
                     /gene="rpmD"
                     /locus_tag="AC16CX_13485"
     CDS             10312..10500
                     /gene="rpmD"
                     /locus_tag="AC16CX_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008540439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3013557.1"
                     /translation="MAKAQGKTLKVTLVRSPIGTKQSHRQCVRGLGLRKLHQTVELPD
                     TPATRGLINTVAYLVECE"
     gene            10566..11063
                     /gene="rplO"
                     /locus_tag="AC16CX_13490"
     CDS             10566..11063
                     /gene="rplO"
                     /locus_tag="AC16CX_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR3013558.1"
                     /translation="MKPNEIKPARGSKHPKKRVGRGFGCHGKTAGRGHKGQKSRAGGF
                     HKVGFEGGQMPLQRRVPKRGFASQLRDDTAEVRLSDLQKLAAEVIDLAALKSAGVVPQ
                     LALRAKVILAGEIKRKVALKGLLASKGARAAIEAAGGSVEAPVAVEPGRLKSKTAGVA
                     AGEGK"
     gene            11110..>11809
                     /locus_tag="AC16CX_13495"
     CDS             11110..>11809
                     /locus_tag="AC16CX_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3013559.1"
                     /translation="MGDLKQRLLFLLGALIVFRVGSFIPVPGIDPEQLAELFRSQRGG
                     ILDMFNMFSGGALSRFTIFALGIMPYISASIIMQLMSVVSPTLEALKKEGESGRRKIT
                     QYTRYGTAALALFQSMGIAIALESQRGLVLEPGLAFRLTTMLTLTTGTLFLMWLGEQI
                     TERGIGNGMSLIIYAGIVVGLPRAVLQTFDQMRTGQIGLIRVLFLLILMVLVVAAIVF
                     VERGHRRVTVQYAKR"
BASE COUNT         2418 a         3676 c         4080 g         1635 t
ORIGIN      
        1 cggcgatcac gcgggtgctg gggaagaagg accccaagat ccagtttcgg tcgttcgact
       61 cgatcgacaa cgcgccggag gagaaggcgc gggggatcac catcgcggtg gcgcacgtgg
      121 agtactcgac ggccaagcgg cactacgcgc acgtggactg tccggggcac gccgattaca
      181 tcaagaacat gatcaccggg gcggcgcaga tggacggggc gattctggtg gtctcggcgg
      241 ccgacgggcc gatgccgcag acgcgcgagc acattctgct ggcgcgccag gtgggggtgc
      301 cctacatcgt ggtgttcatg aacaaggcgg acatggtcga tgacaaggag ctcctggagc
      361 tggtggaggt ggaggtgcgg gagctgctgt cgaagtatga ctttccgggg gacaagaccc
      421 cgattgtgat cggttcggcg ctcaaagcga tggagggcga caagggggag ttgggcgagg
      481 gggcgatcat gaagctggcg gaggccctgg atgcctacat tcccacgccc aagcgggtga
      541 tcgacaagcc gtttttgatg ccggtggagg acgtgttctc gatttcgggg cgcggcacgg
      601 tggtcaccgg gcgggtggag cgcggggtgg tgaaggtggg cgatgagatc gagatcgtgg
      661 ggctgcgccc cacgcagaag acggtgtgca cgggggtgga gatgttccgc aagctcctcg
      721 atgaggggca ggcgggggac aacgtgggga tcctgctgcg cggcaccaag cgcgaagagg
      781 tggagcgcgg ccaggtactg gcgaagcccg ccacgatcac gccccacacc aagttcaccg
      841 ccgaggtcta cgtgctgtcc aaggaagagg gcgggcggca cacgccgttt ttcaacggct
      901 atcggccgca gttctatttt cgcaccaccg atgtcaccgg ctcgatcgag ctggcacagg
      961 gcgccgagat ggtgatgccg ggggacaatg tgtcgatcac ggtctcgctg atccagccca
     1021 ttgcgatgga ggaaggcctg cgcttcgcca tccgcgaggg cggcaagacg gtcggcgccg
     1081 gcgtggtggc gaaagttatc gagtgattga gtgactaagt gattgagtga ttaagaggca
     1141 gacgatccac acaatcacta aatcactatt cacctaatca ctggggttga aaatggcaac
     1201 ggtaaaaagt cagaaaatcc gcatccggtt gaaggcgttc gactatcgcc tgatcgatca
     1261 gtcagcgctc gagatcgtgg acaccgccaa gcgcaccggc gcggtggtga agggaccggt
     1321 gccgctgccg acgcgcaagc agcgcttcga cctgctgcgc tcgccgcacg tggacaagaa
     1381 atcgcgcgaa cagctcgaga tccgcacgca cctgcggctg atggacatca tcgaccccac
     1441 cgacaagacg gtggacgcgc tgatgaaact cgatctgccg gcgggagtgg acgtcgagat
     1501 caaattgtaa atgcgtgagt cgtggatcga atcacgaatc acgaatcacg gggttaagag
     1561 gcaagaacat gagtttgggt ttagtagggc gcaaggttgg catgacccgg gtgttcaccg
     1621 acgagggcga cgccgtcccg gtgaccgtgg tggacgtgtc gaacaatcgc gtgtcccaga
     1681 tcaagacggc cgagaccgac ggctatgccg cggtgcaggt cgcgttcggc cggcggcgcc
     1741 cggcccgggt caaccgggcc gcggcgggac atttcgccaa ggcgggggtg gaggccggcc
     1801 acacgctgcg ggagtttcga gtcaagccgg acgaggtcgc caacctgaag gtcggcggcg
     1861 cgatcggcgc ggacctcttc aagatcgggc agaaagtgga cgtctccggg accacccagg
     1921 gcaagggttt caccggcgtc atcaagcgcc accacttttc ctcgaaccgc gcgagccacg
     1981 gcaattcggt gtcgcacaac aaaccgggct cgatcggcca gaaccaggac ccgggccgcg
     2041 tttttccggg caagcgcatg gccggccaca tgggcgccgc ccgccgcacc gtgcagaacg
     2101 tcgagatcat ccgcatcgac accgagcgcc agttgctgct gatcaagggc gcgctgcccg
     2161 gctcccgcgg cggcggcgtg acggtgaagc cgaccacccg cgcggcgcgg gcggctgcgc
     2221 cggccaagcc tgccgccaag ccggcgaagt aaggggacac catggaactg ccggtgatca
     2281 acgagcaagg caagcctacc gcgacagtcg ccgcctccga cgcggcattc gcgcgcgaat
     2341 acaacgaatc gctggtgcat caggtcgtca cggcctacct ggccaacgcg cggcagggca
     2401 cccgggcgca gaaggcgcgc ggcgaaatca ataaatccaa caagaagccg tggcgacaga
     2461 agggcaccgg ccgcgcgcgc gccgggcggg cttcctcgcc cctgtggcgc ggcggcggca
     2521 agatctttcc gaacctgccg gacgagaact tcagccagaa gttgaaccgc aagatgtacc
     2581 gcgccggggt ttcctcgatc ctgtcccagc tcgcgcgcga ggaacggctg gcggtggtgg
     2641 aaaagttcac ggtggacgcg cccaagacca agctcctgat ggcgaagttg aagggcatgg
     2701 ggctcgatga cgtgctgatc atcaccgaca gcctggacga gaacctgcgg ctgtcctcgc
     2761 gcaacctacc caacgtcgac gtggtgacgg tgggtctcgc cgatccgctg tcgctgatgc
     2821 ggcacgccaa ggtgctggtg accaagggcg cggtcgcgaa gatcggggag ctattcgcat
     2881 gacaaccgca acgccgaata tccagcagca agcccgcctg atgcagatcg tcctcgcgcc
     2941 ggtgatctcc gaaaaaagca cctttgtcgc ggacaagcac aaccaggtga tcttccgggt
     3001 cgcctcgggg gccaccaagc ccgaaatcaa ggcggcggtc gagttcatgt tcaaggtcca
     3061 ggtgcagtcg gtgacggtcg ccaacgtacg cggaaagagc aagcgtttcg gccagtacac
     3121 cggtcgccgc aaccactgga ggaaggctta cgtccgcctc aagccgggcc aggaaatcaa
     3181 ctttgccgaa caggcggagg ccaagtaatg ctggtcaaga tcagacccgt cacacccggc
     3241 cagcgccagc aggtcaaggt cgtcaatccg gaactccaca aaggcgggcc ggtgccgcac
     3301 ctcgtcgagc gcaagatccg gaaaggcggc cgcaacatgc acggcgagat caccatgcgc
     3361 caccagggcg gcggccacaa gcagcattac cggaaagtcg acttcaagcg cgacaaggac
     3421 gccatcacgg cgagggtcga gcgcatcgag tacgatccca accgcagcgc gtttctcgcg
     3481 ctgctgtgct acgcggacgg tgagcggcgc tacatcatcg cgccgaaggg cgtctcggcg
     3541 ggcgcgcaac tcgtctccgg gccggacgcc ccgatcaagg ccggcaacac gctgccgctg
     3601 aggaacatac cggtcggcac caccgtccac tgcgtcgaga tgctccccgg caagggcgcg
     3661 caacttgcgc gcgcggccgg cgcctcggtg cagctgctcg cgcgcgaggg cgattacgcg
     3721 caggtgcggc tgcgctcggg cgagatccgc aaagtgcaca tcaattgccg ggccaccatc
     3781 ggcgaggtcg gcaacgagga gcattcgctc gaatcgatcg gcaaggcggg gcgcgtgcgc
     3841 tggcgcggca tccggcccac ggtgcgcggc gtggcgatga acccggtcga tcatccgcac
     3901 ggcggcggcg agggcaagaa ggccgccgcg cgggacccgg tttcgccgtg ggcggtgctg
     3961 accaaaggct acaagacgcg caagaacaag cgcaccgacg cgatgatcat ccgccgccgc
     4021 aacgccaaga ccgtcggagg ctgatccatg gcacgctcga tcaagaaggg gccgttcgtc
     4081 gatctccacc tcatcaagaa agtggaggcg gcgcaaaaga cgaacgacaa acgcccgatc
     4141 aagacctggt cgcgccgttc gaccatcctg cccgacttcg tgggcctcac catcgccgtg
     4201 cacaacggca ggcagcacgt gccggtgtac gtcaccgaaa acatggtcgg ccacaagctg
     4261 ggcgagtttt cgcacacccg tatcttcaag ggccactcca aggccggttc cgcgcaggct
     4321 gccgcggcgg ccgcggcggc cgcggcgggt ggcgacagga gatccgcgcc gacgccggcc
     4381 gctgcgccgg ccgctgcgcc agcggccccg gctgccccgg cacccaaggc ataaggggca
     4441 ggcatgaata ccactgccaa attgcgcggc gtccgtctct cggcccagaa gggccggctg
     4501 gtggcggatc tcatccgcgg aaaaccggtg gagcaggcgc tcaacatcct gcagttcgcg
     4561 cccaagaagg gggcggtgat catccgcaag gtgctggagt cggccatcgc caacgccgag
     4621 cacaacgatg gcgccgacat cgacgagctg aagatcacga acattctcgt cgagcgcggg
     4681 actttcctgc gccgcttccg cgcgcaatcg aagggccgcg gcgcgcgcat caacaagccg
     4741 acctgcaaca tcttcgtgac cgtcggcgac gggagaaagt agagatgggc cagaagatca
     4801 atccaatcgg tttccggctc gcggtgaacc gcaactgggc ctcgcgctgg tacgccaaca
     4861 acaagaactt ctcgagcatg ctgctcgagg acatcagggt gcgcgactac ctcaagaaaa
     4921 agctcgccca tgcggcggtg tcgcgggtcg tgatcgagcg cccggcgaag aacgcgcgca
     4981 tcacggttca cagcgcgcgc cccggcgtgg tgatcggcaa gaagggcgag gacatcgagt
     5041 tgctcaaggg cgagctgcag aagatgctgg gcgtgccggt gcacgtcaac atcgaggaag
     5101 tgcgcaaacc cgagaccgac gcgcagctga tcgcggattc gatcgcccag cagctgcaga
     5161 agcgcatcat gttccgccgc gcgatgaagc gcgccatcac caacgcgatg cgcctgggcg
     5221 cccagggcat caagatcgcg agcgcgggca ggctcaacgg tatcgagatc gcgcgctacg
     5281 agtggtatcg cgaggggcgc gtgcccctgc acacgctgcg cgcggaaatc gattacggcg
     5341 tctccgaggc aaagaccagc tacggcgtca tcggcatcaa ggtctgggtg ttcaagggtg
     5401 aagtgctggc gaagagcgat cagccggcag cggcgatcgc cgccgcgctc gcggcgccgg
     5461 ccgcggcgcc tgcggcggtg gacaagcccg cacccgcggc cgcgcccggc ggtgccgcca
     5521 aggacaaggc gaagaaaccg cgcgcgccgc gcaaatcgcc ggccaagtca ggagcaaagc
     5581 atgctgcaac ccgcaagacg taaataccgc aaggagcaga agggccgcaa ccgcgggatc
     5641 gcgacccgcg gcaacaaggt gagcttcggc gagtacgggc tcaaggcgac cacgcgcggg
     5701 cgcctcacgg cgcgccagat cgaggcggcg cggcgcgcga tgtcgcgcca cgtcaagcgc
     5761 ggcgggcgca tctggatccg catcttcccg gacaagccgg tgtcgcagaa accggcggaa
     5821 gtgcgcatgg gcaacggcaa gggcaacccc gagttctggg tatccgagat caagcccggc
     5881 gcggtgctct acgagatgga cggcgtgaac gagcaggaag cccgcgaagc gctccgcctg
     5941 gcggcctcca agctcccgct gcgcaccacg tttgtttcgc ggctggcggg ggggtaaacg
     6001 atgaaggcag ccgaactgaa ggcgaaatca ccggatgagc tgcggcagga gcttaatgcc
     6061 ctgctcaagg cgcagttcag cctgcgcatg cagaaggcca cccagcagct caccaatacg
     6121 agccagctgg gcaaggtacg gcgcgacatc gcgcgcgtgc gcacggctct ggccgggaaa
     6181 gggcagggca aggcatgagc gaagcggcga acaagcgcca gctgaccggc cgcgtggtca
     6241 gcgacaagat ggcgaagacg gtgaccgtgc tggtggagcg gcgcatgaag cacccgctct
     6301 acggcaagtt cgtcacgcgc tcccggaagt accacgccca cgacgaggcg aacgagttca
     6361 aggagggcga tctcgtgttg atcgaggaat gccgcccgat ctcgaggacc aaggcctggc
     6421 gcgtgatccg gctggtggag aaggcgaaga ttgtttgaca gggtgatttt gcgttgcgga
     6481 accaggggga tgcccgtata attcgcaccc ctccagcgac ccggctgatc cgcgcggggc
     6541 gcactgcagg aagcagcatc gccgccccgt tgagggcgcg gcgaccgacc cggacgggct
     6601 ccaatactgg ccgcgaggcg gcaaagttgg agaaaaagga ttcaagcgag acgagcaatg
     6661 atacagatgc aatccgtatt ggacgtggcc gacaacaccg gcgcccgttc ggtgatgtgc
     6721 atcaaggtac tgggcggctc gaagcgccgc tacgccggca tcggcgacgt catcaaggtg
     6781 agcgtgaagg acgcggcgcc gcgcgggcgc gtgaagaagg gcgagatcta cgacgccgtg
     6841 gtggtgcgca ccgcgaaggg cgtgcgccgc aacgacggct cggtgatcaa gttcgattcc
     6901 aacgcggcgg tgctgctgac gcccaaactc gagccgatcg gcacgcgcat cttcgggccg
     6961 gtgacgcggg agctgcggac cgagcgcttc atgaagatcg tctcgctcgc gccggaagtg
     7021 ctgtgaggga gcgggccatg cgcaagatcc gaaaaggcga cgacgtcgtc gtgctcgcgg
     7081 ggcgcgacaa gggcaagcgc ggcaccgtgc tcagaatcgt ggacgcggag cacgtgctgg
     7141 tcgagggggc gaaccgggtg aaaaagcacc agcgcccgat cccgatgaag ggcatcaccg
     7201 cgggcatcat cgacaaggag atgccgctgc acgtctcgaa cgtggccctc tacaacccgg
     7261 gaaccaagaa gggcgaccgc gtcggcatcc gggtgctgga agacggccgc cgcgtccgct
     7321 atttcaagtc caacggcgaa gtgatcgacg catgagctca gccaagacca gggacgcgag
     7381 ggacgccaag gaagccaagg gcgccaagga agccaaaggc cagaaggaag ccaaagccgg
     7441 cgcgaaagac gcccgggctc cgaaagaagc caggcagccc gcgccgccca aagaggcgag
     7501 ggaagcgaaa ccggaagcaa gagaggctcc ggcgcaaaag gagcagaaag aacgcaagcc
     7561 agagcgcccc gctcccgtgc cgcggctgca gatacattac cgcgacgcgg tagtgaagca
     7621 attgatgcaa cagttcggtt acaagaccgc gatgcaagtg ccgcggatcg agaagatcgt
     7681 gctcaacatg ggcgtgagcg aggcggtcgc cgacaagaag ataatggaca acgcggtcgc
     7741 cgacatggcc aagatcgccg gccagaagcc ggtcgtcacc aagtcgcgca agtcgatagc
     7801 ggtattcaag atccgcagcg gctacccgat cggctgcaag gtgacgctgc gcggccggcg
     7861 catgtacgag ttcctcgacc gcctggtcaa catcgcgatg ccgcgtatcc gcgacttccg
     7921 cggcatctcg gggcgtgcct tcgacggcca gggcaactac aacatgggcg tcaaggagca
     7981 gatcatcttt cccgagatcg agtacgacaa gatcgacgcg atccggggca tgaacatcac
     8041 catcaccacc accgcgaaat ccgacaacga ggcgaaggcg ctgctcagcg ccttccgctt
     8101 cccgttcagg aactgacagg ccccgaggta taacctatgg caaaaaccgc cgtgatcaac
     8161 cgcgataaga agcgccgcga aaccgtcaag aagtacgccg cgcggcgcaa ggagctgctg
     8221 gagatcgtca acaaccagaa gctttctccg gaagagcgct atgcggggcg cgagaagctg
     8281 cagaagctgc cgcgcgacgc ctcgccggtc aggctcagga accgctgcgc gctgaccggg
     8341 cgcccgcgcg gcgtctaccg caagttcggg ctcgcgcgcg ggaagctgcg cctgatcgct
     8401 ctgcacggcg agatcccggg cgtgatcaag gcgagctggt aacaaggagt cagtatggcc
     8461 atgaccgatc ccatcgccga catgctcacg cgcatccgca acgcccagtg tgcggaaaag
     8521 cagtcggtgg cgatccccgc gagcaaggtg aagacggcga tcgcgcaggt gctcaaggac
     8581 gagggctatg tcgaggactt cgcggtgcgc gagctggacg gcaggcccac gctcgagatc
     8641 aacctcaagt actacgccgg caagccggtg atcgagaaga tcgagcgggt gagccgcccg
     8701 ggactgcgga tctaccgccc caaggacgag ctgccgaaag tgatgaacgg gctcggcgtc
     8761 gcgatcgtct cgacctcccg cggcgtgatg accgaccgca aggcgcgcgg gctgggcgtc
     8821 ggcggcgaag tgctgtgcat cgtggcgtaa cggagcatcg tcatgtcgag aatcggaaac
     8881 gcccccgtca cgttgcccga caaagtcgag gtggcgatca cgccgggcga gatctcggtg
     8941 aagggcccgc tcggcgcgct cagccggaag ctctcgcccg aggtcaaggt ggaaaaggtg
     9001 gaaaaccgca tccagttcaa gcccgcggga aactcacggc tggccaacgc catgtccggc
     9061 acgctgcggg cgctcgtcgc caacatggtg accggggtca ccaagggctt cgagaggcgg
     9121 ctcacgctgg tgggcgtggg ctaccgcgcg caggcgcagg gcgacaagct gaacctcacg
     9181 ctcggtttct cgcaccccgt cgtgtatccg atgccgaaag gcatcaaagt cgagacaccg
     9241 acgcagaccg agatcgtgat caagggcatg gaccggcagc tcgtcggcca ggtggcggcg
     9301 gaagtgcgcg cctggcgcag ccccgagccg tacaagggca agggcgtgcg ctataccggc
     9361 gaggaggtcg tgatcaagga aaccaagaag aagtaacggg gacgctcatg tttgacagga
     9421 accggcagcg tttgcgcagg gcgcgccaga cccgctccaa gatcgcggag ctggcggcgg
     9481 tgcggctcac cgtgcaccgc tcgaacagcc acatctacgc gcaggtgatc gacccgagcg
     9541 gcgcgaaggt gctcgcggcc gcctccacga tcgagccgga agtgcgcgga gccgtgaaat
     9601 acggcggcaa cgtcaaggcg gcggccgagg tcggccgtcg catcgccgag aaggcgaaga
     9661 agctcggcat cgaaaaggtc gctttcgacc gcgcgggcta caggttccac ggccgggtca
     9721 aggcgctggc ggatgccgcg cgcgaggccg gcctcaagtt ttaagggctc caagggactg
     9781 aaccatggcg aaactacagg catcgaaaat gcagagcggc gaggaccggg gcgacggtct
     9841 gcgcgagaaa atggtcgcgg tcaaccgcgt caccaaggtg gtgaaaggcg gccgcgtgct
     9901 cggcttcgcg gcgctggcgg tggtcggcga cggcgacggc ggcatcggca tgggcaaggg
     9961 caaggcgcgc gaagtgccgg tcgcggtgca aaaggcgatg gaagaagcgc ggcgcaaact
    10021 ggtcaaggtg agcctcaagc acggcacgct gcagcacgcg gtcatcggcc atcacgggtc
    10081 gtcgcgcgtc tacatgcaac cggcctccga cggcaccggc atcatcgccg gcggcccgat
    10141 gcgcgcagtg ttcgaggtca tgggcgtgca caacgtgctt gccaagtgcc tgggctcgac
    10201 caatccgtac aacgtggtgc gcgccaccat caacgggctc accagcatga acacgccggc
    10261 ccagatcgcc gccaagcgcg gcaagtcggt cgaggacatc acggggtaac catggccaag
    10321 gcacaaggca agacgctgaa agtgacgctg gtgagaagcc cgatcggcac caagcagtcg
    10381 caccgccagt gcgtgcgcgg gctgggcttg agaaagctcc accagaccgt ggaactgcct
    10441 gacacgccgg ccacccgcgg cctgatcaac acggtggcct acctggtgga gtgcgagtag
    10501 gaatttctgc gcaagtgact gcgcagaaat tccttgattg aatacatctg gatcagaacg
    10561 gtactatgaa gcccaatgag atcaagccgg ctcgcggctc caagcatccg aagaagcggg
    10621 tggggcgcgg cttcggctgc cacggcaaga ccgcggggcg cggccacaag gggcagaagt
    10681 cgcgcgccgg cggcttccac aaagtcggct tcgagggcgg ccagatgccg ctgcagcgcc
    10741 gggtgcccaa gcgcggtttc gcctcgcagc tgcgcgacga caccgccgag gtgcggcttt
    10801 ccgacctgca gaagctcgcg gcggaggtga tcgatctcgc ggcgctgaag agcgcgggcg
    10861 tggtgcctca gctcgcgttg cgcgccaagg tgatactcgc cggagagatc aagcgcaagg
    10921 tcgcgctgaa aggactgctg gcgtccaagg gcgcccgcgc cgcgatcgag gccgccggcg
    10981 gcagcgttga agcgccggtc gcggtggagc cgggcaggct caagagcaag acggccggcg
    11041 tcgcggctgg cgaaggcaaa taaggaccgg gctttggctg ccaccgattc cctgctgggc
    11101 ctgggcaaga tgggcgacct gaagcagcgc ctgctgttcc tgctcggcgc gctcatcgtc
    11161 tttcgcgtcg gctccttcat cccggtgccg ggcatcgacc ccgagcagct cgcggagctg
    11221 ttccgctccc agcgcggggg catcctcgac atgttcaaca tgttctcggg gggcgcgctc
    11281 tcgcgcttca ccatcttcgc gctcggcatc atgccctaca tttcggcctc gatcatcatg
    11341 cagctgatga gcgtggtctc gcccacgctg gaggcgctca agaaggaggg ggaatcgggg
    11401 cggcgcaaga tcacccaata cacccgctac ggcaccgccg cgctcgccct gttccagagc
    11461 atgggtatcg cgatcgcgct cgaatcgcag cgcggtctcg tgctcgagcc gggcctcgcg
    11521 ttccggctca ccaccatgct caccctcacc accggcaccc tgttcctgat gtggctcggc
    11581 gagcagatca ccgagcgcgg catcggcaac ggcatgtcgc tcatcatcta cgccggcatc
    11641 gtcgtcggcc tgccgcgcgc cgtgctgcag accttcgatc agatgcgaac cggccagatc
    11701 gggctcatcc gggtcctgtt cctgctgatc ctgatggtgc tcgtcgtggc cgcgattgtg
    11761 ttcgtcgagc gcgggcatcg gcgggtcacc gtgcaatacg ccaagcgcg
//
LOCUS       DBNGWZ010000444         5715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1188115, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000444 DBNGWZ010000000
VERSION     DBNGWZ010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1188115"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16CX_13500"
     CDS             <1..1010
                     /locus_tag="AC16CX_13500"
                     /inference="COORDINATES: protein motif:HMM:NF052847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3013560.1"
                     /translation="FAPLAGRLERIRLKAGDPVEQGQVVALLTPVAPALLDARTAREL
                     QARVGAAEAQQLRARAEVARVLAQRDQAKADFDRQVKLYKEGFLSATAREQAELALRT
                     AERTVEASRFAEDAAGHELNQARAALARYRSGEAGSKWEVTSPVKGSVLKVVQESEGA
                     VALGAPLLELADARSLEAVVDVLSQESVAIRPGMPARIELGRGVAPLAARVRLVEPAA
                     FTKISALGVEEQRVNVVLDFAEPLDRIQTIGDGFRVDAAIITHRVADAVKVPVGALFR
                     DGEGWAVFVADGGRAVKRAVRATQRNGTEAMVEDGLRPGERVVVYPSDALRDGSRIRA
                     R"
     gene            1093..2082
                     /locus_tag="AC16CX_13505"
     CDS             1093..2082
                     /locus_tag="AC16CX_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013561.1"
                     /translation="MKTVLDINQLALYALTACLIAVSISAFAQDYPTKPVRLVVGSTP
                     GGGTDISARMIAPKLSEYLGQQVVVENRPGATTMIGVEFVARAAPDGYTLLMGVSSLT
                     INPYFQTKVPYDAVKDFAPVSQVIVAPNVIVAHPSLPVRTVKELVAFAKTRPGQLNFA
                     AGGVGSNQHLAIELFMSMTGLKIVHVPYKGQGPALIDVVAGHVHLMMSNILSALPHIK
                     NRKIRAIGVTSLKRATVAPDIPTIAEAGVPGYEVVQWYGVLAPANTPRDIIARLHAGT
                     VRAVQDPLIRQRVINDGGETVGSTPEEFAAVIRADLRKWGQLVKDAGIKPDQR"
     gene            2376..3293
                     /locus_tag="AC16CX_13510"
     CDS             2376..3293
                     /locus_tag="AC16CX_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013562.1"
                     /translation="MSSAQPYPSKPVRLIVPTAPGGGTDISARLIAPKLSELLGQPVV
                     VEDRAGGNTSVGNEFVAKSPPDGYTLLMGISSIAINPHTQSKVPYDPVKDFAPISQVV
                     TVPLVLASHPSLPTRTLKELIAFTRARPGQLNYGTGSVGSNPHLAMELFLTMTGLKVT
                     HVPYRGQGPAMIDLLAGHLELMMANLLTALPHMRSNRIRSYGVTSTARSAVAPEVPTI
                     AEAGVPSYEVVQWFGILAPAGTPREIINRLHAATVRALQDPVVKQRFLADGADTIGNT
                     PEQFAAIIRSDLTKWGKLVKAAGIKPDQR"
     gene            complement(3420..3584)
                     /locus_tag="AC16CX_13515"
     CDS             complement(3420..3584)
                     /locus_tag="AC16CX_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013563.1"
                     /translation="MSGMRLFWSTCPKCMKAFVVAWELRHANRQLICPYCGHRYLPDE
                     SAAIDERHAD"
     gene            complement(3581..5029)
                     /locus_tag="AC16CX_13520"
     CDS             complement(3581..5029)
                     /locus_tag="AC16CX_13520"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HIR3013564.1"
                     /translation="MPEPKKSGAKAGGGSAPRSLGSYLDRIRDDPAEFRTVSRTVNPA
                     NFDVTALLEHLDRRREYPAVQFNNPLNLHGEPSKFPIVTNLFGTRERCAEAVGLARSE
                     AGARLGSLLCERIDRKIEPVVVSSDSAPVHANVLQGEKADMWMLPAVRHFEMDLGTVL
                     TMGMIAHAPGENFYNITFVKAFPETGQRAGLTVHTLHMSRMLKLWEHRGERLPVAYVL
                     GHHPAFWLGTLNNTPWGDNEYATCGGFLQEPVRLAPSVTWGKDFLVPADAEIIIEGEI
                     VPGERTVVNPFGDISTQYQAQQLGPVMEARAITYRDGALFQNIFSGHREHMLLGSIPR
                     EGTIYNQLQHKLGNVVAVHMPYSGCARFTAYISIRKTEEGQAKTAGLQALAHVPNLLA
                     AVVVDEDIDVFNEEHVLWAVNFQVDPSRDISLITNMRPPSDPRGLGSARVIIDATRPT
                     HIAFPTRLRVPQDAMTRVRLEEWLDAVKGGVR"
     gene            complement(5212..>5715)
                     /locus_tag="AC16CX_13525"
     CDS             complement(5212..>5715)
                     /locus_tag="AC16CX_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3013565.1"
                     /translation="FASAGNLSVGHMAGELFKHYAKVDMTHIAYKGGGPAVIATVSGE
                     TAIYFGGPSVVQQSKAGQLRAVAATGTRRSRVFPELPTVAETLPGYAIAQWVGVLAPA
                     ATPRDIVSILHATIAKVLSSARVTEQFTNAGSERIASSPEEFAAHIKTEIAKWRKVLK
                     GSNVPLE"
BASE COUNT          953 a         1884 c         1901 g          977 t
ORIGIN      
        1 tgtttgcgcc gctcgccggg cgcctcgaga ggatccgcct caaggcgggc gacccggtcg
       61 agcagggaca ggtggtggcg ctgctgacac cggtggcccc cgcgttgctc gatgcgcgca
      121 ccgcccgcga gctgcaggcg cgcgttggcg cagccgaggc gcagcagctg cgcgccaggg
      181 ccgaggtcgc gcgcgttctg gcgcagcgcg accaggcgaa agccgacttc gatcgccagg
      241 tgaagctcta caaggaaggc tttctctccg cgaccgcgcg cgagcaggcc gagctcgcgc
      301 tgcgcaccgc cgagcgcacc gtcgaggcgt cgcgattcgc cgaggacgcc gcggggcacg
      361 agctcaacca ggcgcgggcg gcgctcgccc gctatcgcag cggcgaggcg ggctcgaagt
      421 gggaggtcac ctcgcccgtc aaaggctcgg tcctgaaagt cgtgcaggaa agcgagggcg
      481 ccgtcgccct cggcgcgccg ctgctcgagc tcgccgatgc ccgcagcctc gaggcggtgg
      541 tggacgtgct ttcgcaggaa tcggtcgcga tccggcctgg catgccggcg cgcatcgagc
      601 tggggcgcgg cgttgcgccg ctcgccgcgc gcgtgcggct cgtcgagccg gcggcgttca
      661 ccaagatttc ggcgctcggc gtcgaggagc agcgcgtgaa cgtggtgctc gatttcgcgg
      721 agccgctcga ccggatccag acgatcggcg acggattccg cgtcgatgcg gcgatcatca
      781 cgcaccgcgt ggcggacgcg gtcaaagtgc cggtgggggc gctgttccgg gatggcgagg
      841 gctgggcggt attcgtggcg gacggcggtc gggcggtcaa gcgcgccgtg cgggcgacgc
      901 agcgcaacgg caccgaggcg atggtcgagg acgggttgcg gccgggcgaa cgcgtagtgg
      961 tctatccatc ggacgcgcta cgcgacggct cccgcatccg cgcgcggtga aggcaaggaa
     1021 gcacaaaggc tttaccgtaa agcgcgccaa gaaaacacca agagtggtat taatataacg
     1081 gttcggaatc atatgaaaac cgttctcgac ataaatcaac ttgcactcta tgcgttgacc
     1141 gcgtgcctca tcgcggtgtc gatcagcgct ttcgcccagg attaccccac caaaccggtg
     1201 cggctcgtcg tgggctcgac gccgggcggc ggcaccgaca tctcggcgcg catgatcgcg
     1261 ccgaagctct ccgagtatct cggccagcag gtcgtggtcg agaaccgccc cggggcaacc
     1321 acgatgatcg gtgttgagtt cgtcgcgcgc gcggcacccg acggctacac gctcctgatg
     1381 ggcgtcagct cgctcaccat caatccctat ttccagacga aggtcccgta cgatgccgta
     1441 aaggacttcg cgccggtctc gcaggtgatc gtggcgccca acgtcatcgt cgcgcatccg
     1501 tcgctgccgg tgcgaacggt aaaggaactc gtcgcgttcg ccaagacgcg ccccgggcag
     1561 ctcaacttcg ccgccggcgg cgtcggttcc aaccagcacc tcgcaataga actcttcatg
     1621 tccatgaccg ggctgaaaat tgtccacgtg ccgtacaagg gccagggtcc cgccttgatc
     1681 gatgtggtgg cgggtcacgt gcacctgatg atgtccaata tcctgagcgc gctgcctcat
     1741 atcaagaacc gcaagatccg cgcaatcggc gtgaccagcc tcaaacgcgc aaccgtcgct
     1801 cccgacatcc cgaccatcgc cgaagccggc gtgccgggct acgaggtcgt gcagtggtac
     1861 ggcgtcctcg cgccggcgaa tacgccacgc gacatcatcg ccaggctcca cgcgggcacg
     1921 gtgcgcgcgg tgcaggatcc cctcatcagg cagcgcgtca tcaacgacgg cggtgaaacg
     1981 gtcggcagca cgccggagga attcgcggcc gtgatccgcg ccgatctcag aaaatggggt
     2041 cagctggtga aggacgcggg gatcaaaccg gatcaacgct gaaagccggc atgcatgccg
     2101 gtcgggggga tatgaaaacc gttctcaaga caattcaact cgcgctccgt gctttgaccg
     2161 cgtgcctcat tgcggtgtca actgccgctc atgcgcaggg ctacccgacc aggccggtgc
     2221 ggctgatcat tccgtttgcg cgagggcggc gcaccggatc tcggcgaggg ggttggtgtt
     2281 ggggcggaga cggggtgcgg ccgccggatt tatggcattg cgctcttcag cgacttgcca
     2341 tcgcgctggt cgcggcggcg tcattcttgg cgggagtgtc gtccgcgcag ccctatccat
     2401 cgaagccggt gcggctgatc gtaccgacgg cgccaggcgg cggtaccgac atctcggcgc
     2461 gtctcattgc tccgaagctc tccgagctcc tcgggcagcc ggtcgtggtt gaagatcggg
     2521 ccggcggcaa cacgagcgta ggcaacgagt tcgtcgccaa gtcgccgccc gacggctaca
     2581 cgctgctgat gggcatcagc tccatcgcca tcaatcccca cacccagtcg aaggtcccgt
     2641 acgatcccgt caaggatttc gcgccgattt cgcaggtggt gacggtgccc ctggttcttg
     2701 cttctcatcc gtcgctcccg acgcggacgc tcaaggagct gattgcgttc acgcgggcgc
     2761 ggcccggcca gctcaactac ggcaccggct cggtcggcag caacccgcac ctcgcaatgg
     2821 agctgttcct caccatgacc gggctcaaag tgacccacgt gccgtaccgg ggccaaggcc
     2881 ccgccatgat cgacctcttg gcggggcacc tggagctgat gatggccaat ctgctcacgg
     2941 cgctgcccca catgcggagc aaccgcatcc gctcgtacgg cgtgaccagc accgcccgtt
     3001 cggcggtagc tcccgaggtt ccgaccatcg cagaggccgg cgtgcccagc tatgaggtgg
     3061 tccagtggtt cggcattctt gcgccggcgg ggacgccgcg cgaaatcatc aaccgcctcc
     3121 atgctgcgac ggttcgtgcg ctgcaggacc cggtcgtcaa gcagcggttt ctcgccgacg
     3181 gcgcggatac gatcggcaac acgccggagc aattcgcggc gatcatccgc tccgatctca
     3241 cgaagtgggg caagctggtc aaggccgcgg ggatcaagcc cgaccagcgc taacgaaggc
     3301 gaaagtcgaa agtcgaaagg cgaaagtatc acccccgcag tgcggggtta cagggctgag
     3361 gtggtcatcc agcgaagctg ggatccctcc gactttcgcc tttcgccttt cgccttgtcc
     3421 tagtcggcgt gccgctcgtc gatggcggcc gactcgtcgg gcaggtagcg gtgaccgcag
     3481 tacgggcaga tgagctggcg gttggcgtgg cgcagttccc acgccaccac gaatgctttc
     3541 atgcacttcg ggcaggttga ccagaagagc ctcatcccgc tcatcgcaca cctcctttga
     3601 cggcgtcgag ccactcttcc agccgcaccc gcgtcatggc gtcctgcggc acccgcaacc
     3661 gcgtcgggaa ggcgatgtgc gtgggacgcg tggcgtcgat gatcacccgg gccgagccga
     3721 gtccccgcgg gtcgctcggg gggcgcatgt tcgtgatgag cgagatgtcg cggctcggat
     3781 cgacctggaa attgaccgcc cacagcacgt gctcctcgtt gaacacgtcg atgtcctcgt
     3841 cgacgacgac cgcggcgagc aggttcggca cgtgcgccaa tgcctgaagg ccggcggtct
     3901 tcgcctggcc ctcctcggtc ttccggatgg aaatgtaggc ggtgaagcgc gcgcaacccg
     3961 aatacggcat gtgcaccgcg acgacgttgc cgagcttgtg ctggagctga ttgtagatcg
     4021 tgccttcgcg cgggatgctg ccaagcagca tgtgctcgcg gtggcccgag aagatgttct
     4081 ggaacagggc gccgtcgcga taggtgatcg ccctcgcctc catcacgggc cccagttgct
     4141 gcgcctggta ctgggtgctg atgtcgccga agggattgac cacggtgcgc tcgccgggaa
     4201 cgatctcgcc ctcgatgatg atctcggcgt cggccggcac caggaaatcc ttgccccatg
     4261 tgaccgaagg cgcgaggcgc acgggctcct gcaggaaacc cccgcacgtc gcgtactcgt
     4321 tgtcgcccca cggcgtgttg ttgagcgtcc cgagccagaa tgcgggatgg tgcccgagga
     4381 cgtacgccac cggtaagcgc tcgccccggt gctcccacaa tttcagcatc cggctcatgt
     4441 gcaaggtgtg gacggtaagt cccgcgcgct ggccggtctc ggggaacgcc ttcacgaagg
     4501 tgatgttgta gaagttctcg cccggggcgt gcgcaatcat gcccatcgtg agcacggtgc
     4561 cgaggtccat ctcgaagtgg cgcacagccg gcagcatcca catgtcggcc ttctcgccct
     4621 gcagcacgtt cgcgtgcacg ggcgcgctgt cgctgctgac gacgacgggc tcgatcttgc
     4681 ggtctatgcg ctcgcacagg agcgacccga gacgggcgcc cgcctcgctg cgcgccagcc
     4741 ccaccgcctc ggcgcagcgc tcccgggtgc caaacaggtt ggtgacgatc gggaacttcg
     4801 agggctcacc gtgcaggttg agcgggttgt tgaactgcac cgccgggtac tcgcgccgcc
     4861 ggtcgagatg ctccagtagc gcggtgacgt cgaagtttgc cggattcacg gtgcggctca
     4921 ccgtgcggaa ttccgccggg tcatcgcgaa tccggtcgag gtaagagcca aggctcctgg
     4981 gcgcgcttcc gccaccggcc ttcgctcccg actttttcgg ctccggcatg cgtgttctcc
     5041 tcattcacgt agctttctgt gaatagtgaa cggccgatca atatccgcac cgactctacg
     5101 ggccagggtt gcccctgtca acacactcgc ggcaagaacg ctgaagcaaa ccaatttcgc
     5161 cctcaagttc gaactgcgtg gtcgcgcggc gcaaccgcca ggcaggcatt tctactcgag
     5221 cggcacattg gaacccttca gcacctttct ccatttggcg atctccgtct tgatgtgcgc
     5281 cgcaaattcc tccggcgagc tggcgatgcg ctcggagccg gcgttggtga attgctcggt
     5341 caccctagcg ctgctcaaga ccttcgcgat cgtagcgtgc aggatcgata cgatgtcgcg
     5401 cggcgtggcg gcgggcgcaa gaaccccgac ccactgggcg attgcgtagc ccggcagggt
     5461 ctcggcgacg gtcggcaatt ccgggaagac ccgcgagcgc ctggtccccg tggcagcgac
     5521 cgcccgcagc tggcccgcct tggactgctg gacgacgctc gggccaccga aatagatcgc
     5581 ggtctcgccg ctcaccgtcg cgatgacggc gggtccgcca cccttgtacg cgatgtgcgt
     5641 catgtccacc ttcgcgtagt gcttgaacag ctcgcccgcc atgtggccga cggagagatt
     5701 gcctgcggag gcgaa
//
LOCUS       DBNGWZ010000445         5124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_938049, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000445 DBNGWZ010000000
VERSION     DBNGWZ010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_938049"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16CX_13530"
     CDS             <1..212
                     /locus_tag="AC16CX_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013566.1"
                     /translation="ATGFSSDFVRLYDAAARKELARVEVGRGSSHSAFLGDTAWIGCS
                     VSDHVAHVDLGARRCTARIQLEDRD"
     gene            251..1036
                     /locus_tag="AC16CX_13535"
     CDS             251..1036
                     /locus_tag="AC16CX_13535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013567.1"
                     /translation="MENGKPRILMSGAGKHFGALDVFRGISLEVGEREVVAIIGPSGC
                     GKTTLLRCIGGLIPLTTGEITVEGEPVTGPREGVAVVFQHFGLFPWKTVFDNVAYGLK
                     LAGASRAEIEEKVPKFISLVGLTGFEDYYPYQISGGMQQRCGVARALSVEPRVLLMDE
                     PFGAVDAQTREILQFEMLRIWETRPTTMVFVTHAIDEAVLMGDRVVVLKGRPSSVHEI
                     VEVDLPRPRDRSTLLQPRFAELREHVWGTLMADARRAERELTG"
     gene            1072..2088
                     /locus_tag="AC16CX_13540"
     CDS             1072..2088
                     /locus_tag="AC16CX_13540"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013568.1"
                     /translation="MKKGRSGCIRNGNVQVACAALALMLLPALAAAQKLTFAIPGIPP
                     VFAGTIALVAEKEGMFKKHGVDVTVRAFETGAAASRAVASGEIAVALSPTPLIVSQVA
                     NTDVKLVGIWGMEHPDWLIGATDPDATCASLKDAPVGVDSVGGARSIALRTMLVACKM
                     KIEEVQQVALSSNVGAAMVAGQLKFGVLHIDDIPVIESESKKPLKIVVRQKDSRPVDH
                     YLLLVAHQDHLAKNRGAYVRIVAALIDAERFMRNPANYAKVAQYAAPTGRSGEIALQA
                     LKDYIAMEFWPKDHDGLGRKNIEVVGNIQVKVGNIKADKKPAPYERIVDTSVWKDASA
                     MVKK"
     gene            2118..2882
                     /locus_tag="AC16CX_13545"
     CDS             2118..2882
                     /locus_tag="AC16CX_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013569.1"
                     /translation="MGPETRRGRYGTMIASLLAGAAVWEIAGRVVSGAFWAPLSATLD
                     SLAETARSGALVRQILSSFELYFSGLALAILAGAPFGLLLARVRWLRIALENYIMMLY
                     ATPMVALIPFILSMMGFGFAPKVLVVFLFAVFPVLYNTVEGARSIRPELVEVAHSFRS
                     SEWRLWCDVMIPYTLPFTMTGIRQATARGLVGMVAAEIFLSPSGLGQMIMMGSQNFDT
                     PGLLAAILIIVILGVALMDMGRFLENRFAAWRGYSR"
     gene            2861..3685
                     /locus_tag="AC16CX_13550"
     CDS             2861..3685
                     /locus_tag="AC16CX_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013570.1"
                     /translation="MAGLQPMTAQTLAAGAGAARAGPRAWVASPLFLKVAAGAALLLA
                     WEATVRALAPAYVAKPSGVAMVIVPVIGDPQFLAAAWATLAAVVKGLAISIVLGTAVG
                     FTMGRVDLAERGLRYYVNSLFATPMVAILPLVTLWFGYSADARLAVVVFAAFFSVAVN
                     AADGARSVPLEYLEIARSFRARPRHTLFDIIFPSSLPYLLAGLRLAAGRALVGAVVAE
                     FFISIPGLGYYILFNSRTFRHNEAFVAVIVLVALGVSFEVLVNWLTRRYLPWARRD"
     gene            3687..4682
                     /locus_tag="AC16CX_13555"
     CDS             3687..4682
                     /locus_tag="AC16CX_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3013571.1"
                     /translation="MNYRRLGTGGPSVSAISMGRGSQAIKFEDDAMIQAFNTTIRRAC
                     ELGINFFDSSDAYWGTRHEVLLGRAIKGFRDQVLISSKFGNIDLPDGKKATNGRPEYV
                     YQSCDASLKRMNVDVIDVYYLHRVDPAVPIEETIGAMARLIEQGKVRYLGVCEAGPQT
                     LRRAYKTHPLAALQTEYSLWFREVERDILPTCRELGISYVAYAPLGRGLLTGRIKTVN
                     DLPPNDRRRRHPRFAPENLARNVKLVEELEAMARGQGVTAAQLALAWLLAQGDDIVPI
                     PGTNHVKNVELNAAAADIRLSAEKIARLSEVFAIGAGAGARYHDVVLKQIAGQLR"
     gene            4711..>5124
                     /locus_tag="AC16CX_13560"
     CDS             4711..>5124
                     /locus_tag="AC16CX_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013572.1"
                     /translation="MKAKGERRKAKGQQMGWFHFRLSTSAFLLCCGLALSAAPVAAQP
                     YPAKPIRLVVPWSAGSGTDLMARAFAQELTKAIGQQVVLDNRGGAGATIGTELAAKSP
                     PDGYVIYVGGSVSMVISPVLYSKVGYDPIRDFAPVS"
BASE COUNT          835 a         1613 c         1786 g          890 t
ORIGIN      
        1 tcgcgacggg gttctcctcc gattttgtac ggctttacga tgccgccgca cgcaaggagt
       61 tggcccgtgt cgaagtgggg cgtggctctt cgcacagcgc cttcctgggg gacactgcct
      121 ggatcggttg ctcggtgagc gaccacgtgg cgcacgtcga tctcggcgcg cgccgctgca
      181 cggcgcggat ccagctcgag gaccgagatt gactgtcaat tggcaaaacc ctaacctgat
      241 gatcgattcg atggaaaacg gaaaaccgcg catcctgatg tcgggcgccg gcaagcattt
      301 cggcgcgctg gacgtatttc gcggaatcag cctcgaagtg ggagagcgcg aggtggtggc
      361 catcatcggc ccctcgggct gcggaaaaac gacgctcttg aggtgcatcg gcgggctgat
      421 cccgctcacc accggcgaaa tcacggtgga gggcgagccg gtcaccgggc cccgcgaagg
      481 tgtggcggta gtcttccagc atttcggcct gtttccgtgg aagaccgttt tcgacaacgt
      541 ggcctacggc ctcaagctgg ccggcgcatc gcgcgccgag atcgaggaga aggtcccaaa
      601 attcatcagt ctggtgggac ttacgggatt cgaggactat tatccgtacc agatctccgg
      661 cggcatgcag cagcgctgcg gtgtcgcgcg cgctctctcg gtcgagcccc gggtcctgct
      721 catggacgag ccgttcggcg ccgtggacgc gcagacccgc gaaatcctgc aattcgaaat
      781 gctgcggatt tgggagacgc gcccgacgac gatggtgttc gtgacgcacg cgatcgacga
      841 ggcggtgctg atgggcgacc gggtcgtggt gctgaaaggg cgcccgagca gcgtgcacga
      901 gatcgtcgag gtcgatttgc cgcgtccacg cgaccgcagc acgctgctgc agccgagatt
      961 cgcggaattg cgcgagcacg tctggggcac attgatggct gatgcgcgcc gggcggagcg
     1021 cgagctgacg gggtagggga acttggttcc actaacgagg aggagaggac gatgaaaaag
     1081 ggcagatcag gatgcatccg caacggcaac gttcaagtcg cttgcgccgc gctggcgctg
     1141 atgttgctcc cggcactggc cgcggcgcag aagctcacgt tcgcgatacc cggcattccg
     1201 ccggtctttg ccgggacgat cgcgctcgtc gcggaaaagg aaggcatgtt caagaagcac
     1261 ggggtcgacg tgacggtccg ggcgttcgag accggtgcgg ccgcgtcgcg cgcggtggcc
     1321 tccggggaga tcgccgtggc gctctcgccg actccgctga tcgtcagcca ggtcgcaaac
     1381 acggatgtga agctggtcgg catctggggc atggagcatc ccgactggct gatcggcgcg
     1441 acggaccccg acgcgacgtg cgcgagcctg aaggacgccc cggtcggcgt ggattccgtg
     1501 ggcggcgcgc gctcgatcgc gctgcggacc atgctggtcg cttgcaagat gaagatcgag
     1561 gaggtgcagc aggtggcctt gagctccaac gtcggagcgg ccatggtggc gggccagctc
     1621 aagttcggcg tgctgcacat agacgacatc cccgtgatcg agtccgagag taagaaaccg
     1681 ctgaagatcg tggtccggca gaaggattcc cgccccgtcg accattacct gctgctggtc
     1741 gcgcaccagg atcacctggc gaagaaccgc ggcgcctacg tgcgcatcgt ggccgcgctg
     1801 atcgacgccg agcgcttcat gcgcaatcct gccaattacg cgaaggtcgc gcagtacgcg
     1861 gcgcccaccg gccgcagcgg cgagatcgcc ctgcaggcgc tgaaggacta catcgccatg
     1921 gaattctggc ccaaggacca cgacggcctg gggcggaaga acatcgaagt ggtcggcaat
     1981 atccaggtca aggtcggcaa tatcaaggcc gacaagaagc cggcgccgta cgagcgcatc
     2041 gtcgacacgt cggtgtggaa ggacgccagc gcgatggtca agaaatagcg ctggataccg
     2101 ccaccgaggg ggtgacaatg ggtccggaaa cgcgccgcgg gcgatacggc acgatgatcg
     2161 cgagcctcct cgccggagcg gcggtgtggg agatcgcggg tcgcgtcgtg tccggcgcgt
     2221 tctgggcgcc cttgtccgcg accctggaca gcctcgccga gacggcgcgc agcggcgccc
     2281 tggtgcgcca gatcctgagc tcgttcgagc tctacttctc cgggctcgcg ctggcgatcc
     2341 tggcgggcgc gccgttcggg ctgctgctcg cgcgcgtgcg ctggctgcgc atcgcgctcg
     2401 agaactacat catgatgctg tacgccaccc ccatggtggc gctgatcccg ttcatcctgt
     2461 cgatgatggg gttcggcttc gcgcccaagg tactggtggt gttcctgttc gcggtattcc
     2521 cggtcctcta caacacggtc gaaggggccc gcagcatacg tccggagctg gtggaggtgg
     2581 cgcattcgtt ccgttcgagc gaatggcgcc tgtggtgcga cgtgatgatc ccgtacacgc
     2641 tgccgttcac catgaccggg atccgccagg cgaccgcgcg ggggctggtc ggcatggtcg
     2701 cggcggagat cttcctgagc ccctcgggcc tggggcagat gatcatgatg ggctcgcaga
     2761 actttgacac cccggggttg ctggcggcga tcctgatcat cgtcatcctc ggcgtggcgc
     2821 tgatggacat gggccggttc ctcgagaacc ggttcgccgc gtggcggggc tacagccgat
     2881 gacggcgcag acgctcgccg cgggcgccgg cgctgcgagg gccggcccgc gcgcgtgggt
     2941 cgcgtcgccc ctgttcctga aggtcgcggc cggcgccgcg ctgctgctcg cgtgggaggc
     3001 caccgtgcgg gcattggcgc cggcgtacgt cgcgaagccg tccggcgtcg cgatggtgat
     3061 cgtcccggtg atcggggacc cccagttcct ggcagccgcg tgggcgacgc tcgcggcggt
     3121 ggtgaaaggc ctcgcgatct cgatcgtgct cggcacggcg gtgggcttca cgatgggccg
     3181 cgtcgatctc gccgagcgcg gattgcgcta ctacgtgaac agcctgttcg cgacgcccat
     3241 ggtcgcgatc ctgccgctgg tgacattgtg gttcggctac agcgccgacg cgcgcctcgc
     3301 ggtggtggtg ttcgccgcgt tcttttcggt cgcggtcaac gctgccgacg gggcgcgctc
     3361 ggtgccgctc gagtacctcg aaatcgcgcg ctcgttccgc gcgcgccccc gccacacgct
     3421 cttcgacatc attttcccgt cgtcgctgcc gtacctgctc gccgggctcc ggctcgcggc
     3481 ggggcgggcg ctggttggcg ccgtggtggc ggagtttttc atctccatcc ccggcctcgg
     3541 ttattacatc ctgttcaatt cccgcacgtt caggcacaac gaggcgttcg tcgcggtgat
     3601 cgtgctcgtc gcgctggggg tcagtttcga ggttctggtc aactggctca cgcgccggta
     3661 tctgccgtgg gcgcgaaggg actgaggtga actatcgccg gctcggcacg ggcggcccga
     3721 gcgtttccgc catcagcatg ggacgcggca gccaggcgat caagttcgaa gatgacgcga
     3781 tgatccaggc gttcaatacg accatccgcc gcgcctgcga gcttggcatc aacttcttcg
     3841 actcgtccga cgcctactgg ggcacgcgcc acgaggtgct gctcggccgt gcgatcaagg
     3901 gctttcgtga ccaggtgctg atttcaagca agttcggcaa catcgacctg cccgacggca
     3961 agaaggcgac caacggccgc cccgagtacg tgtaccagtc ctgcgacgcg agcctcaagc
     4021 gcatgaacgt ggacgtgatc gacgtctact acctgcaccg ggtggatcca gccgttccga
     4081 tcgaggaaac gatcggcgcg atggcgcggt tgatcgagca agggaaggtt cgatacctcg
     4141 gagtctgcga ggcggggccg cagactctca ggcgcgcata caagacacac ccgcttgcgg
     4201 cgctgcagac cgagtattcg ctgtggttcc gcgaggtcga gcgcgacatc ctgccgacct
     4261 gccgcgagct cggtatctct tatgtcgcgt acgcgccgct cgggcggggg ctcctcaccg
     4321 gccggatcaa gaccgtgaac gacctgccgc cgaacgaccg caggcgccgc cacccgcgct
     4381 ttgcgcccga gaaccttgcc cgcaacgtga agctggtgga ggaactggaa gccatggcgc
     4441 ggggacaggg cgtgacggcg gcccaacttg cgctcgcctg gctgctcgcg cagggcgatg
     4501 acatcgtgcc gatacccggc accaaccatg tcaagaacgt ggagctgaac gcggccgccg
     4561 ccgacatccg gctgtcggcg gagaaaatcg cccggctcag cgaggtcttt gcgatcggcg
     4621 ccggcgccgg cgcgcgctat cacgatgtcg tgctgaagca gatcgccggc caacttcggt
     4681 gatgactgcc gagtgacgag tgaggaaatc atgaaggcga aaggcgaaag gcgaaaggcg
     4741 aaagggcaac agatgggctg gtttcacttc cgactttcga cttccgcctt cctactttgc
     4801 tgcgggctgg cgctctctgc tgcgccggtc gcagcacagc cgtatccggc gaagccgata
     4861 cggctggtcg tgccgtggtc tgccggcagc ggcaccgacc tcatggcgcg cgcgttcgcg
     4921 caggagctga ccaaggcgat cggccagcag gtcgtgctgg acaaccgcgg cggcgccggc
     4981 gcgacgatcg gaacggaact tgccgcgaag tcgccgcccg acggttacgt gatctacgtc
     5041 ggcgggtcgg tctcgatggt gatcagcccg gtgctctact ccaaggtcgg ctatgacccg
     5101 atccgcgatt tcgcgccggt ctcg
//
LOCUS       DBNGWZ010000446        23980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1568611, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000446 DBNGWZ010000000
VERSION     DBNGWZ010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23980
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1568611"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16CX_13565"
     CDS             <1..265
                     /locus_tag="AC16CX_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013573.1"
                     /translation="QYPVTYECNPGTGALRRYWNYAIAAAQSTPPVTPNNALLATSIS
                     ACGFSYITGAGTGRTGLVSLSLQVSRDGETVRLFQQVHVSNVP"
     gene            285..803
                     /locus_tag="AC16CX_13570"
     CDS             285..803
                     /locus_tag="AC16CX_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX family protein"
                     /protein_id="HIR3013574.1"
                     /translation="MIRDSSIRTGFAERGFSIVTAVFILVVLALLAAFIVSVTGLQQS
                     TAQLDLQGVRAYQAARAGIEWGAYQTLDPNNTIGTPALPACPASPTNMSGLAASLSVF
                     TVTVTCSLTSTTEGNRNVGAYQIVSTACNQPSGGACPNPTPSTGYVERQLQATLSKCK
                     DPTAAGPRFACG"
     gene            912..3962
                     /locus_tag="AC16CX_13575"
     CDS             912..3962
                     /locus_tag="AC16CX_13575"
                     /inference="COORDINATES: protein motif:HMM:NF042738.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HIR3013575.1"
                     /translation="MAANKTWLSGGGANATNWNRGANWSPAGAPGSADDIIIPTTPAN
                     GTGFPVLNATSTIASLTIQSGASLTGASGFNLTVSGNVSITSTGVLNVNASTITVGGA
                     ITGTGTVNGNTSTLNVGGNMTVTTFTAGTSTVVYNGTGAQTAGGAYTYNNLTINKGGG
                     TATLGGATTVNGTLTLTSGTLAVGANTLTLNGPAIAGTPSNLSTTSSSSLSFGGTAAG
                     VSVPSSVTQLNNLTINNGNGITLNSSPTINGTLTLSAGVVSTGANTLITTANCPGSVA
                     RTSGHVAGFLRLQVPTGSPTCTFDVGDSTTYRPMNLTFSSVTTAGNLAGSVSQSAGDH
                     PNISTSGLDATQSVNRFWTLTNSGIALSSYNATFNFVAGDVDAGADTAQFEVERWDGT
                     SWSTTTAGTRTATSTQATGITSFSDFAVAKKKPAAVTPSSYNAFETSTSAGAITGVIK
                     TKIAGASFSLDVVAIAGGAQLAGFTDQVIVELLGNNSLGVPLDAQNCPTSFTLVQTVS
                     PNPTITGGRSTVNFAAVPNSWRDVRVRVRWPTSSPTVTGCSTDNFAIRPNAFSNFSVT
                     DNDAQTAGTGRTLNDTAFGTVTHKAGRPFSVRATAVNAAGTPATTTNYSESPSTTITT
                     CSAGAGTEACTVSFGTLSLNTTFSSGQLVSDVATYGEVGSFQLQLVDSAFSSVDASDG
                     STAAERNIVSSTINVGRFVPDHFAVSLNTPVFGTACSAGTFSYVGQAFNYTVQPVVTV
                     TAQDFGNSTTMLYAGSWWRITNSSLTGKSYTAAAGTVDTSGLPGTDPVIAPAGSGTGT
                     LTFSSGTGLLFTRTAPVAPFDAEIRLEINVLDSDGVAASSNPIAFGAASPGNGISFNN
                     GKQMRFGRLQLGNANGSQLINLRVPLEAQHWNGTSFLTNTADSCTAIGGASIALSNYT
                     QNLNACETSVSISGSFSSGRGNLQLTAPGNANNGGVVLTVNLGASGSGSTCIAGASTP
                     VTGANKPFLQGNWTGGAYDQNPAARATFGVYKGSEEFIYFRENF"
     gene            4082..5035
                     /locus_tag="AC16CX_13580"
     CDS             4082..5035
                     /locus_tag="AC16CX_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="agglutinin biogenesis protein MshI"
                     /protein_id="HIR3013576.1"
                     /translation="MRLFGSTRQRPGWLCINLGPDRVDVSHVLATGRARPEVLLCESY
                     RKEGDDVATLAHLRRELHLDRYRCTTLLRSGDYQMVQVDAPNVPAAEAKNAVRWRIKD
                     AIDYPAEAATVDALFVPAVEGAAGRNPQMFAVAARNELIAATVKPFNDADIPLEAIDI
                     PELAQRNVAHCFEQEGRGLAVLAFGPHGGLITFTCGGELYQYRRIQYRPIDASPAGSA
                     DVHAEQKQGFYNRVVLELQRSLDNFDRQFRQIAIARVLVTPVPGADDMQQYLAAHLDI
                     PVMLLDLSEVMDFPHIPELQGPLRQAQCLQLIGAAMREEAI"
     gene            5139..5798
                     /locus_tag="AC16CX_13585"
     CDS             5139..5798
                     /locus_tag="AC16CX_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013577.1"
                     /translation="MSQNINLFNPAFRKKRQLLTLATVMQCLVITLVALFGYHYFLQQ
                     QVRGLTEELQIAEGLLKSQRVYVDRLKGRAAAPKTDAALDADIARLENELKLAREAME
                     ALKGGAFGNQQGFAEYLRAFSRQSLNGLWLTGFTIAGSGEIEIRGRVLSPDLVPSYIQ
                     RLNREQVLAGRSFARLEMSRPKAEPAADKDKEAKKALRPPRYLEFSLATTDAAKAEKT
                     P"
     gene            5795..6484
                     /locus_tag="AC16CX_13590"
     CDS             5795..6484
                     /locus_tag="AC16CX_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013578.1"
                     /translation="MKEQWQKLAARFDALMMRERLLVLVAAVVGVALIADTIAIRPLE
                     ARKKRLEQQVAESRQNIRAADTVMKAQEAVVDPDTVKRSYRDALRKQLAEIDKSMQGL
                     QRGLVPPERMAKLLEEMLSRSRGLQLISLRTLPAQRFETPGAAPPPKAGDKGAKPAPN
                     APERAIYQHSFEIMLQGSYADLHDYLAQLEKLPWQMFWGRISVNTEQYPRLRVMLTVQ
                     TLSLSKAWLIV"
     gene            6469..7059
                     /locus_tag="AC16CX_13595"
     CDS             6469..7059
                     /locus_tag="AC16CX_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013579.1"
                     /translation="MADRMKTGRRKAEGRGQKKIARSAGSSLVSDPGPPRCIASGVRR
                     LERAQARSSNSRLTLHPSFFALCLALFAPGTAAAQVVTDPTRPPAGYAAPETEFAGDS
                     SVMVLQSVMISPTHKAAIINGVVVKLGEKYGDAVLIKVTENEVVLKSDGASQVLKLHP
                     AVDKREIAPAAEKSGPRRGKARGGAAPAASGSAQPR"
     gene            7073..8806
                     /gene="mshL"
                     /locus_tag="AC16CX_13600"
     CDS             7073..8806
                     /gene="mshL"
                     /locus_tag="AC16CX_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HIR3013580.1"
                     /translation="MRRRILVLAMAIAAVAGCAEPPARRDATYERISAEIDKAVKERA
                     KPAAPDTVSQALLPPLVVEMPRVEAKPLDQRFDLNVNNAPAAQVFMAIVSGTRYSMIV
                     HPDVRDTITVNLKDVTVLEALDTLRDLYGYEYRVQGNRITVSPVTMQTRVFKVNYLQA
                     QRVGRSDVRVSSGSITDAPTTTTGAPGATTPGAPAQRLTESTRVTTSYDSNFWGDIVK
                     SLNAIVGTADGRSVIVNAQSGVIVARALPAELRSVESFLKAMQLIVERQVVLEAKIID
                     VTLRDSYGAGINWAAFRDGSTLAAGGLIRPGATIGNTGALSTPTARAPDGSPAADSTF
                     TATLGAAASSLAAGVGAPGAVFGLALQTSNFAALLTFLETQGSVNVLSSPRVATINNQ
                     KAVLKVGQDDFFVTNVSTTSTTTGTSATVSPTITVQPFFSGIALDVTPQIDENNNIIL
                     HIHPQVSRVVEQRKVVDLGTLGTFTLPLASSSINETDTIVRVQDGNIVAIGGLMRQES
                     TSDVSQVPGIGDQPGIGALFRQRGRTNAKSELVILLKPTIVHSDRNWQQDLAETRDRV
                     RALEPPQPPQQ"
     gene            8825..9715
                     /locus_tag="AC16CX_13605"
     CDS             8825..9715
                     /locus_tag="AC16CX_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR3013581.1"
                     /translation="MYRSHFGLREMPFGITPDTSFAYACNTHQEALNTLLVAVKNGEG
                     FIKITGEVGTGKTLLCRRFLAKLDQNHVSAYIPNPYLEPRTLLFALAEELGVALARST
                     DQHQLMKELTRALLNFARQGKTVVLCLDEAQAMPVETLEALRLLTNLETEKRKLLQVV
                     LFGQPELDQKLEHPSIRQLRQRITFQYRLTALTRDEVDHYITHRLRVAGYRGNRLFTW
                     QATRLVHRFSGGVPRLVNIMAHKAMLLAYGEGMQQVLPLHVRKAAADTPLTHQRRPWW
                     WLGFAMLLISVGGIGWMVLS"
     gene            9819..10997
                     /locus_tag="AC16CX_13610"
     CDS             9819..10997
                     /locus_tag="AC16CX_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013582.1"
                     /translation="MSVINQVLLDLEKRRASGSELGALPNHVRALPEGERTLHWGWVA
                     GGGVAVAAVVAVSWALLSGFDWTRQRTTAVQTPQRGNEVAIGKVATAAAGTATDVRPR
                     DEGGGAQMEAPASRLSLELSSPSAAAGQAAEQVVADERSPLPTTRVVGRTESDSSPRS
                     DTPAAPAGRPAPAKSAAVVASSKAMASTPAARPEIQKEVRQPTPRDLAENEYRKATAS
                     LHQGRQAEAQEGFQAALNLHAGHHGARQALVGLLLDGKKYSEAERVLQEGLKLAPSQT
                     GFTMTLARLQVDRGDNKQAIATLQRGLEYAQGNADYAAFLAALLQRDGQHEEAIEQFQ
                     AALRLRPASGVWWLGLGISLQAINRAAEAQDAYRRARATNNLSPDLQAFADQRLLQLQ
                     "
     gene            complement(11108..11815)
                     /locus_tag="AC16CX_13615"
     CDS             complement(11108..11815)
                     /locus_tag="AC16CX_13615"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR3013583.1"
                     /translation="MSNFIPGLLNNCLSLARILLPQSCLLCGADSTDEPLCPPCLTDL
                     PWLPRRCCEVCALPLASGKVCGTCLERPPRFDRVEAIFAYRFPVDALIHAFKYRGRLA
                     LARILGELLAQRVARDVDAIVPMPLARGRLAERGFNQALEIAREVAACTGMPLLPAAC
                     RKVADTPPQAALPWKERARNVRRAFVCDVDLDGKRIAVVDDVLTTGATLNELARVLRR
                     AGAREVRGWVVARTLPR"
     gene            11918..12883
                     /gene="bioB"
                     /locus_tag="AC16CX_13620"
     CDS             11918..12883
                     /gene="bioB"
                     /locus_tag="AC16CX_13620"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR3013584.1"
                     /translation="MTAADNASVARRWDPREVEGLYELPFNDLIHRAHGVHRRHFDPN
                     AVQLSTLLSVKTGGCPEDCAYCPQAARYQTGVANEEMLSVERVVAAARLAKRNGATRF
                     CMGAAWRGPKQRELERVIEMVRAVRAEGLETCATLGLLKKGQAEQLKAAGLDYYNHNI
                     DTSPEFYGGIITTRTQDDRLETLERVRQAGLHVCCGGIIGMGESRRTRAALIAQLASM
                     DPYPESVPINNLVQVPGTPLHGTEKLDPLEFVRTIACARITMPRAMVRLSAGRQEMSE
                     AVQALCFFAGANSIFYGEKLLTTGNPDVEQDRALFAKLGLRPLHR"
     gene            12935..14185
                     /gene="bioF"
                     /locus_tag="AC16CX_13625"
     CDS             12935..14185
                     /gene="bioF"
                     /locus_tag="AC16CX_13625"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HIR3013585.1"
                     /translation="MTNSTLDTRHSTLQQALAAELGELESRDLKRHRRLLESPQGARV
                     RVDGRDYIAFCSNDYLGLASHPELVEAARQGAARYGVGAGASHLILGHSAAHHELEQR
                     LAAFVGLPRALLFSTGYMANIGVVSALVGREDAVFADRLNHASLNDAALLSRAAFKRY
                     PHLDLGSLARLLASSRARRKLIVTDAVFSMDGDIAPVPGLIDLCDRYDAWLLLDDAHG
                     FGVLGPEGRGVLARFRARSPCVIYMATLGKAAGVFGAFVAGETEVVELLIQRARAYIY
                     TTATPALLAQALMKSLELVRAEEWRRERLRALAQRLRECLQPARWRLAHSDTAIQPLV
                     IGGNDEAVAVSEALARRGLLVPAIRPPTVPQGTARLRISLSAAHSEQDVEKLAAALRE
                     IERTVPGSEFRVPSSALKADGSTR"
     gene            14169..14954
                     /locus_tag="AC16CX_13630"
     CDS             14169..14954
                     /locus_tag="AC16CX_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013586.1"
                     /translation="MALPGNTQRPTRNPKRRHDIRDVVLLHGWGSGPGIWDDFARLLK
                     PRFRVHALPLPGYGADTEPATRSLRATVAAVARAAPRRCGVVGWSLGGEVALAWARCA
                     RHQVRRVALIASTPCFTSRPGWPSATAPAVLQEFGRSLLADRAGTLARFTEVQAKGDA
                     LARRVACVLQRFYEQSASTDVLVAGLDVLASTDLRRELSRVRQPALVLHGARDRIVPP
                     AAGRRLAAALPDARFVLLRACAHAPFLSKPGEMAAALREFFDE"
     gene            14947..15825
                     /gene="bioC"
                     /locus_tag="AC16CX_13635"
     CDS             14947..15825
                     /gene="bioC"
                     /locus_tag="AC16CX_13635"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HIR3013587.1"
                     /translation="MSDALALDKRLVRRSFEQAAATYDAAAVLQNEVCRRMLGRLDYI
                     RLAPATILDAGCGTGNAVAGLLARYPRARIVAIDLALAMARRARARHPWWRGWLERGG
                     PRLTAVCGDIEQLPLAPGCADLVWSNLAVQWINEPRRAFAEMHRALAAGGLLLFSSFG
                     PDTLKELRASFEGLDHHTHVHRFIDLHDVGDMLVACGFADPVMDMEIVTLTYADVQGL
                     MRDLKAIGAHNMTCGRPAGLGGRSLLARVAQNYEAFRREGRFPATFEVVYGHAWKPQP
                     RMTADGKPIIPLKPLR"
     gene            15963..16640
                     /gene="bioD"
                     /locus_tag="AC16CX_13640"
     CDS             15963..16640
                     /gene="bioD"
                     /locus_tag="AC16CX_13640"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HIR3013588.1"
                     /translation="MAKGYFVTGTDTGVGKTLVACALLRALAARGLAAVGMKPVASGA
                     ELRGNELVNNDVEQLIAASSVVAPRSHVNPYCFTPPVAPHIAAQQAGVTIMLDRIEQS
                     FHALAARAQVVVVEGVGGFRVPLGAGSDTSQLAVRLALPVVLVVGARLGCLNHALLTV
                     EAITTRGLRLAGWVANHLDPQMAAADENVLALQALIAAPLLARIGYASNPEPALIATS
                     LDIGKLL"
     gene            16711..17235
                     /locus_tag="AC16CX_13645"
     CDS             16711..17235
                     /locus_tag="AC16CX_13645"
                     /inference="COORDINATES: protein motif:HMM:NF021517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR3013589.1"
                     /translation="MIHSTGFRRMWAGHTMAAGMQCSETAHAAEFSWVARRNNSLSSS
                     GRHLVFAFLLVVSVGIAAAFAYFGAWLVLPFAGLEMLVLYLAFRYIERHAADYELVAI
                     DGDRVKIEICEAGRARSHELNRYWAQLVSRDGGRLALRSHGREVEIGRHMNEDERLAL
                     ARELGRQLRGGVVH"
     gene            17293..18417
                     /gene="coxB"
                     /locus_tag="AC16CX_13650"
     CDS             17293..18417
                     /gene="coxB"
                     /locus_tag="AC16CX_13650"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR3013590.1"
                     /translation="MISKGLRSFAAALAWLAAETALANRYNLQEPQSIIAREIYDLHT
                     LIMWIIVGIFVVVFGSMTYAIVKHRKSVGHKAEQFHENTTVEIIWTIIPFFILIGMAY
                     PATRTVIAMKDTSGSDITIKATGYQWKWGYDYLQEGISFYSNLATPREQIEGRAPKGE
                     NYLVEVDNPVVVPVGKKVRIIATANDVIHSWWVPALGIKQDAIPGFVRDTWFKADSPG
                     IYRGQCAELCGKDHGFMPIVVEAVTPERYASWVAEQKKKMAAAAPEPGKQWTLDELKA
                     HGEKIYAGNCVACHQPTGMGVPGTFPALSGSKVVNGPKEGQIQVLLNGVVKDGKPTAM
                     VSFKHLSDVDIAAVITYTRNNWANKTGEMVAPADVKAARK"
     gene            18453..20042
                     /gene="ctaD"
                     /locus_tag="AC16CX_13655"
     CDS             18453..20042
                     /gene="ctaD"
                     /locus_tag="AC16CX_13655"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR3013591.1"
                     /translation="MEAAHAHNVHGHGEHGHHYTGIMRWITTTNHKDIGTLYLWFALT
                     MFFVGGVLALMLRAELFEPGMQFWRPEFFNQLTTMHGLIMVFGAIMPAFVGFANWQVP
                     MMIGAPDMAFARMNNWSFWLLPVGATLLVASFFVPGGATAAGWTLYAPLSTQMGPGMD
                     LAIFALHIMGASSIMGSINIITTILNMRAPGMTLMKMPLFVWTWLITAYLLIAVMPVL
                     AGAVTMVLTDRHFGTHFFNAAGGGDPVLFQHVFWFFGHPEVYIMILPGFGIVSQIVPT
                     FARKPLFGYSSMVYATASIAILSFIVWAHHMFTVGMPAAAQLFFMYATMLIAVPTGVK
                     VFNWIATMWKGALTFETPMLFACGFIFVFSMGGFTGLVLAITPVDIQLQDTYYVVAHF
                     HYVLVAGSLFALFGGTYYWLPKWIGRMYDETLGKWHFWLSMIFFNVTFFPMHFTGLAG
                     LPRRVPDYNLQFADFNMISTIGAFGFGLSQLIFVYVLWKCISGAGEKVSDRVWEGAHG
                     LEWTLPSPPPYHTFETPPVVK"
     gene            20283..20372
                     /locus_tag="AC16CX_13660"
     CDS             20283..20372
                     /locus_tag="AC16CX_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HIR3013592.1"
                     /translation="MTSNLKTALILASIAAAFFIGVVIRHWLW"
     gene            20366..20905
                     /locus_tag="AC16CX_13665"
     CDS             20366..20905
                     /locus_tag="AC16CX_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR3013593.1"
                     /translation="MVSHSHANQQTLVKLLVVAVLMFGFGFALVPFYRAICDALGLNS
                     VVKADAVANTQVDTGRSLTVEFDTNLRSNLPWTFTPLEKSVRIHPGALTQVTFEIRNR
                     SDHPVTGQAIPSFGPQLAGRYFKKLECFCFTQQTLAPGEVRRMPVVFVVEPGLPRDVN
                     TVTLSYTFFEVEGKTGKGS"
     gene            20905..21120
                     /locus_tag="AC16CX_13670"
     CDS             20905..21120
                     /locus_tag="AC16CX_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HIR3013594.1"
                     /translation="MLMAEQAQPPRKATPLQVAKAVLSAFIGIRKRAAHEQDAVTLSP
                     LQVIVAGVIAAAVFVLSLVMLVRYITS"
     gene            21136..21984
                     /locus_tag="AC16CX_13675"
     CDS             21136..21984
                     /locus_tag="AC16CX_13675"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR3013595.1"
                     /translation="MLNQSARYFIPDPSTWPITGSIALLCMASGAASWFNGWSPGKFI
                     LLAGVAILAYMLVGWFHTVSHESEGGKYNKQVDVSFRWGMSWFIFSEVMFFGAFFGAL
                     FYMRVLSVPWLGDFEHKLIWPDFTATWPATGPGLKDPFTPMAAWGIPAVNTLLLLSSG
                     VTVTLAHWALIRNDRTKLIAWMFATIGLGVMFLAFQIYEYGHAYSELNLKLTTGAYGS
                     TFFMLTGFHGFHVTIGCIMLTTILFRCVAGHFRPDHHFGFEGVAWYWHFVDVVWLGLF
                     IFVYWL"
     gene            complement(22188..22385)
                     /locus_tag="AC16CX_13680"
     CDS             complement(22188..22385)
                     /locus_tag="AC16CX_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HIR3013596.1"
                     /translation="MKFVVILFLVFIVGSLASALYFMVKDRGQGERTVKALTVRVALS
                     VALFALLMLGFYFGLIPGKLE"
     gene            22437..23159
                     /locus_tag="AC16CX_13685"
     CDS             22437..23159
                     /locus_tag="AC16CX_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR3013597.1"
                     /translation="MSARYRFQPRLITTLAAAGGIVLTLALANWQLGRAHEKEALGRR
                     LEALAKDPPVWLPAAEVRAEDVEWRRVTARGRFEPKHAVFIDNRVRHGVAGYHVVMPL
                     AIGGGSRYVLVNRGWVTGSPDRARLPQVKTPEGTVEITGLAVTPSRRFLELSTHVAEG
                     NVWQNLTLERYRQAVPIALQPVVIQQESPLDDGLVREWDPPNLGVEKHYGYAFQWLAL
                     ALTILVFYLVTHVRRRSPEEAH"
     gene            23125..23700
                     /locus_tag="AC16CX_13690"
     CDS             23125..23700
                     /locus_tag="AC16CX_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3013598.1"
                     /translation="MSDAAPRKRLTSLWLIIAVAAAPVVASYLLYYFWPPERTVNHGE
                     LVEPRPLPDPELALADGRPFRLSQLKGKWVLITVDSGRCDAHCERKLTYMRQLRLTQG
                     KEMERVERAWLISDGIAPRPETVAPYHGTWLVRAAGTELLKAFPAQGAASDHIYIVDP
                     LGNLMMRFPRDPDPRLMIKDLARLLRASRIG"
     gene            23773..>23980
                     /locus_tag="AC16CX_13695"
     CDS             23773..>23980
                     /locus_tag="AC16CX_13695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760735.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3013599.1"
                     /translation="MSSSLARQPLASRFSQFYQLTKPRVVSLIVFTAVIGMFLATPDM
                     VPLQALVAGTLGIALTAGAAAAVNC"
BASE COUNT         4314 a         7782 c         7759 g         4125 t
ORIGIN      
        1 gcaatacccg gtgacctacg aatgcaaccc cggaaccggg gcgctgcggc gctactggaa
       61 ctatgcgatc gccgcggcgc agtcgacgcc gcccgtcacg ccaaacaacg cactactcgc
      121 gaccagcatc tccgcgtgcg ggttttccta cataacgggc gcggggacgg ggcgcaccgg
      181 cctggtgtcg ttgagcttgc aagtatccag ggacggcgaa accgtgagac tgttccagca
      241 ggtgcatgta agcaatgttc cctaacatcc gtgagtcgtg attcgtgatt cgtgattcga
      301 gtatcaggac tggctttgcg gagcgcggct tcagcatcgt gaccgcggtc ttcatcctcg
      361 tagtgctcgc gctgctcgcc gcgttcatcg tctcggtgac cggcctccag caatcaaccg
      421 cacagctcga tctgcagggc gtacgggcct atcaggcagc gcgcgccggg atcgaatggg
      481 gcgcctatca gacgctcgac cccaacaaca cgattggcac gccggcgctg ccggcatgtc
      541 ctgcgtcgcc gaccaacatg agcggtctcg cggcttcgct gtcggtcttt accgtgaccg
      601 tgacctgcag tctgacctcc accaccgagg gtaaccgcaa tgtcggcgcc taccagatcg
      661 tttcgacggc gtgcaatcag ccgtccggcg gcgcgtgtcc caatccgacg ccctccaccg
      721 gttacgtcga gcgccagttg caggcgacgc tttccaagtg caaggacccg accgcagccg
      781 gaccgcggtt cgcatgcggt tgatgcggga tgaaacagat tatgtatctc gtccaaagac
      841 tgccaggaat gttcgctagg ctcgctctat cgagtctttt cgggctcgtg ttgctgggtg
      901 ccgagcctgg cttggccgcg aacaagactt ggttgagtgg aggcggggca aatgcgacca
      961 attggaaccg aggggcgaac tggtcgccgg ccggcgctcc gggttcagcc gatgacatca
     1021 tcattccgac gactcccgcg aatggcaccg gattcccggt cctcaacgcg acctccacca
     1081 ttgcgagcct cacgatacaa agcggcgcga gtctcaccgg cgcgagcggc ttcaacctaa
     1141 ccgtgagcgg taacgtatcc attacgagta ccggtgtgct aaatgtcaat gcaagcacga
     1201 tcacggtggg cggcgcaatt accggtactg gcactgtcaa tgggaataca agcacgctga
     1261 atgtcggcgg caacatgacc gttacgacgt ttacggcggg cacgtcaact gtggtctata
     1321 acggcactgg cgcacagacg gccggggggg catacaccta caacaacctg acaatcaaca
     1381 aaggaggggg caccgcgact ctcggcggcg ccacgacggt gaatggcacc ttgacactga
     1441 cgagcggtac gctggcggtg ggggcgaaca cgctcaccct caacggtccg gccattgcgg
     1501 gaacgccttc caacctctcg accacctcat cctccagcct ctctttcggg ggcaccgcgg
     1561 ccggagtgag cgtgccgagc agcgtgacgc aactcaacaa cctgacgatc aacaacggca
     1621 acggcattac cctgaatagc agcccgacca tcaacggcac gcttaccctc agcgccgggg
     1681 ttgtgtctac cggggcgaac accttgatca ccacggccaa ctgcccgggc agcgttgcgc
     1741 gcactagcgg gcacgtggca ggtttcctca ggctgcaagt cccaaccggg tcaccaacct
     1801 gcacgtttga tgtgggagac agcaccacct acaggccaat gaatctgacg ttttccagtg
     1861 tcaccacggc ggggaacctg gcggggtcag tcagccaaag cgcaggcgac catcccaaca
     1921 tcagcacatc gggcctcgac gccacccaga gcgtcaaccg cttttggacg ctgaccaata
     1981 gcgggatcgc tctgtccagc tacaacgcca cgttcaactt cgttgccggc gatgtggacg
     2041 cgggcgcgga taccgcccag ttcgaggtgg agcgctggga cgggaccagt tggtccacaa
     2101 ccactgcggg cacgcgaacg gccacgagca cccaggcgac ggggataaca tccttcagcg
     2161 acttcgcggt ggcgaagaag aagccggcgg cggtcacacc gagcagctac aacgccttcg
     2221 agaccagcac gtccgccggc gcgatcaccg gcgtgatcaa gacgaagatt gccggcgcga
     2281 gcttcagctt ggacgtcgtg gcgatcgcgg gcggcgcgca gctcgccggc ttcaccgacc
     2341 aggtgatcgt cgagctgctc ggcaacaaca gtctgggtgt cccgctcgat gcccagaact
     2401 gcccgacctc tttcacgctg gtgcagacgg tttcgcccaa ccccacgatc acgggcggac
     2461 gttcgacggt gaacttcgcg gcggtgccga attcgtggcg cgacgtgcgg gtgcgggtga
     2521 gatggccgac ttcgtctccg accgtgaccg gctgctcgac cgacaacttc gcgatccggc
     2581 ccaatgcctt cagcaatttc agcgtcaccg acaacgacgc gcaaacggcg ggaaccgggc
     2641 gcaccctgaa cgatactgca ttcgggaccg tcacccacaa ggccggccgg ccgttcagcg
     2701 tgcgggccac ggcggtcaat gccgccggca ccccggcgac caccacgaac tacagcgaat
     2761 ctccgagcac aacgatcacg acctgttctg cgggcgcggg aaccgaagcg tgcacggtga
     2821 gcttcgggac gctctccctc aacaccactt tttcttcggg gcagctcgtt tccgatgtcg
     2881 caacctatgg cgaagtcggc tcgttccagc tgcaactcgt ggattcggct ttctccagcg
     2941 tggatgcgtc cgacggctca acggcggctg agcgcaatat cgtctcctct acgatcaatg
     3001 tcggccgttt cgtgcccgac cacttcgcgg tgtcgctcaa tacgccggtg ttcgggaccg
     3061 cgtgcagtgc cggcaccttc tcctatgtgg gccaggcgtt caattacacg gtgcagccgg
     3121 tggtcacggt gacagcgcag gatttcggca acagcacgac gatgctctac gcaggctcat
     3181 ggtggcgcat cacgaattcc agtctgaccg gcaagagcta cacggctgcc gccggcaccg
     3241 tcgataccag cgggctgccc ggaaccgacc cggtgattgc gccggccggt tccggcaccg
     3301 gcacgctgac ctttagctct ggcaccgggc ttctcttcac ccgcacggcg cctgttgcgc
     3361 cattcgatgc ggagatccgg ctggagatca atgtgctcga tagtgacggc gttgccgcat
     3421 cgagcaaccc catcgcattt ggcgcagcga gccccggcaa cggcatcagt ttcaacaacg
     3481 gcaagcagat gcgcttcggg cgactgcagc tgggcaacgc caacggatcc cagctcatca
     3541 acctgcgggt gccgctcgaa gcccagcact ggaacggcac atcgttcctc accaacaccg
     3601 ccgacagctg caccgcgatt ggcggggcaa gcatcgcgct gtccaattac acgcaaaacc
     3661 tcaatgcctg cgaaaccagc gtgtccatca gcggcagctt ttcatccggc cggggcaacc
     3721 tgcagctcac tgcgccgggc aacgccaaca acggcggggt ggtcctcacg gtgaatctcg
     3781 gcgcaagcgg tagcggctcg acctgcatcg ctggtgcttc gaccccggtg acaggagcaa
     3841 acaagccgtt tctacagggg aactggacgg gtggcgccta tgaccagaat ccggcagcgc
     3901 gcgcgacttt cggcgtttat aaaggcagcg aggaattcat ttatttccgg gaaaacttct
     3961 agttattaca gtccgttatc agtgtagtcc tgcgggtccg ctcaaaaaat attcgccaag
     4021 ggtttgacat tattgaattt tatggtagtt tctgccaatt agtcgtaata ggccctgaaa
     4081 aatgaggctg ttcggttcca ccaggcaaag accgggctgg ttatgcatca acttgggacc
     4141 tgaccgcgtg gatgtgtcac acgtgctggc cacggggcgg gcgcggccgg aagtgttgtt
     4201 gtgcgagtcc tatcgcaagg aaggcgatga cgtagcgact cttgcgcacc tgcgccgcga
     4261 gctgcacctc gaccgctatc gatgcaccac gctgctgaga agcggcgact accagatggt
     4321 gcaggtggac gcccccaacg tgccggcggc ggaagcgaag aacgcggtgc gctggcgcat
     4381 caaggacgcg atcgactatc cggcggaggc ggcaacggtg gatgcgctgt tcgtcccggc
     4441 ggtcgaggga gcggccgggc ggaaccccca gatgttcgcg gtggccgcgc gcaacgagtt
     4501 gatcgccgcg accgtcaagc cgttcaacga cgccgacatc ccgctcgagg cgatcgacat
     4561 accggaactc gcgcagcgca acgtcgcgca ctgcttcgag caggagggcc ggggactcgc
     4621 cgttctcgcg tttggcccac acggtggctt gatcaccttc acctgcggcg gcgagctcta
     4681 tcagtaccgc cgcattcagt accgccccat cgatgcctcg cctgcgggtt cagccgatgt
     4741 ccacgccgag caaaagcaag gcttctacaa tcgcgtcgtg ctcgagctgc agcgctcgct
     4801 cgataatttc gatcgccagt tccgccagat cgcgatcgcc agggtgttgg tgacgccggt
     4861 accgggcgcc gacgacatgc agcaatatct ggcagcgcat ctcgatatcc cggtaatgct
     4921 gctcgacctc tccgaggtga tggatttccc gcacataccc gagctgcagg ggccgttgcg
     4981 tcaggcgcaa tgcctgcagt taatcggcgc cgcgatgcgg gaggaggcca tatgagctgc
     5041 ctcctcttgg gtttcaagtt tcaagtttca agtttcaagt tatgccggcg ccgacacgaa
     5101 ggcgcttgtc cgtcctggag agtatttgag gtgccggcat gagccagaac atcaatctgt
     5161 tcaatcccgc cttccgcaag aagcggcaac tgttgacgct cgccacggtg atgcagtgtc
     5221 tagtgattac gttggtcgcg ctgtttggct atcactactt cctgcagcaa caggtcaggg
     5281 gattgaccga ggaactgcag atcgcggagg gcctgctcaa gtcgcagcgg gtctacgtgg
     5341 acaggctcaa gggcagggcc gcggcgccca agaccgacgc ggcgctggat gctgacatcg
     5401 cacggctgga aaacgagctc aagcttgcgc gcgaggcgat ggaggcgctg aaaggcggcg
     5461 cgttcggcaa ccagcagggc ttcgccgaat acctgcgcgc cttctcaagg cagtcgctaa
     5521 acgggctgtg gcttaccgga ttcaccatcg ccggcagcgg cgagatcgag attcgcggac
     5581 gcgtgctgag cccggatctc gtgcctagct atatccagcg actgaaccgg gagcaggtgc
     5641 ttgcgggccg cagcttcgcg cggctcgaga tgagccggcc caaggccgaa cccgcggccg
     5701 acaaggacaa ggaggccaag aaagccctgc gtccgccgcg ctatctcgaa ttcagcctgg
     5761 ccaccacgga cgcggccaaa gcggagaaga cgccatgaag gagcaatggc aaaagctcgc
     5821 cgcccggttc gatgccctga tgatgcgtga gcgcttgctg gtgctggtgg cggcggtcgt
     5881 cggcgtagcc ctgattgcag atacgattgc aatccgtccc ctggaagcgc ggaaaaaacg
     5941 cctggaacag caggtcgccg aatcgcgcca gaacatcagg gccgcggaca ccgtgatgaa
     6001 ggcgcaggaa gcagttgttg accccgacac cgtcaagcgc tcgtatcgcg acgcgctgcg
     6061 caagcagctc gccgagatcg acaagagcat gcagggactg cagcgcggcc tggtgccgcc
     6121 cgagcgcatg gcgaagctgc ttgaggaaat gctctcgcgg agccgcgggc tgcagctcat
     6181 ttcgctgcgc accttgccgg cgcagcgttt cgagacaccc ggtgcggcgc cccctcccaa
     6241 agcgggcgac aagggcgcga agcccgcgcc aaacgcaccc gagcgcgcca tttatcagca
     6301 cagctttgaa atcatgctgc aaggctctta cgccgacctg cacgattacc tcgcgcagct
     6361 cgagaagctg ccgtggcaaa tgttctgggg ccgcatcagc gtgaataccg agcaatatcc
     6421 caggctgcgg gtgatgctga cggtgcagac gctgagcctg agcaaagcgt ggctgatcgt
     6481 atgaagaccg gaaggcggaa ggcagaaggc agagggcaga agaagatcgc gcggagcgcg
     6541 gggtcatcct tggtcagtga ccccggcccg ccgcgctgca tcgcgtctgg cgttcgccgg
     6601 ctcgaacgtg cgcaggcacg ttcttcgaat tcccggctca cccttcatcc ttcattcttc
     6661 gcgctgtgcc ttgcgctgtt tgcgcccggc acagcggcgg cgcaggtggt gaccgacccc
     6721 acgcgcccgc ccgcaggata cgcggcgcct gaaaccgaat ttgcgggcga ttcaagcgtg
     6781 atggtgctgc agtccgtgat gatttcgccg acgcacaagg ccgcgatcat caatggcgtg
     6841 gtggtgaaac tcggcgaaaa gtacggagat gccgtcctga tcaaggtgac ggagaacgaa
     6901 gtggtgctca aaagcgacgg cgcgagccag gtgctcaagc tgcacccggc cgtggacaag
     6961 cgcgagatcg cgccggccgc ggagaaaagc ggcccgcgac ggggcaaggc gcggggcggc
     7021 gccgcgcccg cggcgagcgg cagcgcgcaa cctcgataga ggaggggcgg acatgagacg
     7081 acgaatcctg gtgcttgcga tggctattgc cgccgtggcg ggctgcgcgg agccgccggc
     7141 gcggcgcgac gccacgtacg aacggatctc cgccgagatt gacaaagcgg tgaaggagcg
     7201 cgccaagccg gcggcgccgg acacggtcag ccaggcgctg ctgccgccgc tggtggtgga
     7261 aatgcccagg gtggaggcca agccgctcga ccagcgtttc gatctgaacg tcaacaacgc
     7321 gccggcggcc caggtattca tggcgatcgt ctccggcacg cgctacagca tgatcgtgca
     7381 ccccgacgtg cgcgacacca tcacggtgaa tctcaaggat gtcaccgtat tggaggcgct
     7441 cgacaccttg cgcgatcttt acggctacga gtaccgcgtg cagggcaacc gcattacggt
     7501 gagcccggtc accatgcaga cccgcgtctt caaggtcaac tacctgcagg cccagcgcgt
     7561 cggccgcagc gacgtgcgcg tgagctccgg ctccattacc gatgcaccga ctaccacgac
     7621 cggggcgccc ggcgctacga cgcccggcgc gccggcgcag cggctcaccg agagcacccg
     7681 cgtcacgact tcgtacgaca gcaatttctg gggcgacatc gtgaagtcgc ttaacgccat
     7741 cgtgggcacg gctgacgggc gcagcgtgat cgtcaatgcg caatccggcg tgatcgtggc
     7801 gcgcgcgctg ccggccgagc tgcgctcggt cgaatcgttt ctcaaggcga tgcaactgat
     7861 cgtcgagcgg caggtggtgc tggaggcgaa gatcatcgac gtcacgttgc gtgacagcta
     7921 tggggcgggc atcaattggg cggcattcag ggatggcagc accctcgctg ccggcggcct
     7981 gatacggccg ggcgcgacca tcggcaacac cggcgcgctc agcacgccga ccgcacgcgc
     8041 gcccgacggc tcccctgccg ccgactccac attcacggcc acgctcggcg cagcggcgtc
     8101 gtcgctggcc gccggcgtgg gcgccccggg ggccgtcttc gggctcgcgc tgcagaccag
     8161 caatttcgcg gcgctgctga cattcctcga aacgcagggg tcggtcaacg tgctgtcgag
     8221 cccgcgtgtc gcgaccatca acaaccagaa ggctgtgctc aaggtcggcc aggacgattt
     8281 cttcgtcacc aacgtctcaa ccacctcgac caccaccggc accagcgcga cggtctcgcc
     8341 gaccatcacc gtgcagccgt tcttctctgg catcgcgctt gacgtgacgc cccagatcga
     8401 cgagaacaac aacatcatct tgcacatcca cccgcaggtg agccgagtgg tggaacagcg
     8461 caaggtcgtg gatctcggca cgctcggcac cttcacgctg ccgctcgcct ccagcagcat
     8521 caacgagacc gacaccatcg tgcgcgtgca ggacggcaac atcgtcgcga tcggcggcct
     8581 gatgcgccag gaatcgacga gcgacgtctc ccaagtgccc ggcatcggcg accagcccgg
     8641 catcggcgcg ctgttccgcc agcgcggccg gacgaatgcg aagagtgaat tggtgattct
     8701 gctcaaaccc acgatcgtgc acagcgaccg caactggcag caggacctgg ccgagacgcg
     8761 cgaccgcgta agggcgctgg agccgccgca gccgccgcag caatgaaccg acgggcctga
     8821 caccatgtac cgcagccatt tcggactccg tgaaatgccg ttcgggatca cccccgacac
     8881 gagcttcgcc tacgcctgca acacgcacca ggaggcgctc aacacgctgc tggtggcggt
     8941 caagaacggc gaaggcttca tcaagatcac cggtgaggtc ggcaccggca agacgctgct
     9001 ctgccgccgc ttcctggcca agctcgacca gaaccacgtc tctgcctata ttcccaaccc
     9061 ctatctcgag ccgcgcacgc tgttgttcgc gctggccgag gagctcgggg tcgcgctcgc
     9121 caggagcacc gaccagcatc agctgatgaa ggagctcacg cgcgcgctgc tcaacttcgc
     9181 tcggcagggc aagacggtgg tgctgtgcct ggacgaggcg caggcgatgc ccgtcgagac
     9241 gctggaagcg ctgcggctgc tcaccaatct cgaaaccgag aaacgcaagc tgctgcaggt
     9301 cgttctcttc ggccagcccg agctcgacca gaagctggag catccctcga tccggcaatt
     9361 gaggcagcgc atcaccttcc agtaccggct gacggcgctc acgcgcgatg aggtcgacca
     9421 ctacatcacc caccggctgc gcgtggcggg ctaccgcggc aaccgccttt tcacctggca
     9481 ggcgacgcgc ctggtgcacc ggttcagcgg cggcgtgccg cggctcgtca acatcatggc
     9541 gcacaaggcc atgctgctcg cttacgggga aggcatgcag caggtgctgc cgctccacgt
     9601 gcgcaaggcg gccgctgaca ctccgcttac gcatcagcgg cggccgtggt ggtggctcgg
     9661 ttttgccatg ctgctgattt cggttggcgg catcggctgg atggtgctgt catgagcggc
     9721 ggagcagggg ccctgcttgc ggggatgcga cccgcgaagg acgcagggcc gccgacgcga
     9781 taccccacga attgcgacta cgtgtcagag gcggcggcat gagcgtgatc aaccaggtac
     9841 tgctcgacct ggaaaagcgc cgtgcgtccg gatcggagct cggggcgctg ccgaatcacg
     9901 tgcgggcgct tccggagggc gagcgcacgc tgcactgggg gtgggtcgcg ggaggcggcg
     9961 tcgcggtggc ggcggtggtc gcggtatcgt gggcgttgct ttccggattc gactggacgc
    10021 gccagcgcac cacggccgtg cagacacccc agcgcggcaa cgaggtcgcc atcggaaaag
    10081 tggcgacggc cgccgcagga acggctacgg acgtaaggcc ccgtgatgag ggcggaggcg
    10141 cacaaatgga agcgccggcc tcgcgtctca gtctcgagct ttccagtccc tccgccgccg
    10201 cgggtcaagc cgcggaacag gtcgtagcgg atgagcgctc cccgctgccg acaactcgcg
    10261 tggtcggccg gacggaatcg gactcctccc cccgcagcga tacgccggca gcaccggctg
    10321 gacgtcctgc gccggccaag tcagccgcag tggttgcgag ctccaaggcc atggcgagca
    10381 ctccggcggc gagaccggaa atccagaaag aagtgcggca gcccacgccg cgcgacctcg
    10441 ccgaaaacga gtatcgcaag gcgaccgcct cgctgcatca gggacgccag gccgaggccc
    10501 aggaaggctt ccaggcggcg ctgaatctcc atgccgggca ccatggcgcg cgccaggcgc
    10561 tcgtgggatt gctgctggac gggaagaagt acagcgaagc cgagcgcgtc ctgcaggaag
    10621 gcctgaaact cgcgccgtcg cagactgggt tcacgatgac gctcgcccga cttcaggtcg
    10681 accgtggcga caacaagcag gcaatcgcca cgctgcaaag aggtctcgag tacgcccaag
    10741 gcaacgccga ttatgccgcc ttcctcgccg cgctgctgca gcgcgatggg cagcacgagg
    10801 aggcgatcga gcagttccag gctgcattgc ggctgagacc cgcttcgggc gtgtggtggc
    10861 tggggcttgg catttcgctg caggcgatca atcgtgccgc cgaggcgcag gacgcctacc
    10921 gccgcgcccg ggccactaac aatctcagtc ctgatttgca ggcattcgcc gatcagcgcc
    10981 tgctgcaatt gcaatagcac tgcaattgca aatcccaagt cccaaatccc aagttccaac
    11041 cctcaaatcc gaacttccaa agattgacgc ttggaatttg tggtctggtg cttggaactt
    11101 gccgtggcta gcgcggcagc gtccgcgcca ccacccaccc cctgacttcc cgggcacccg
    11161 ccctgcgcaa gacgcgcgcg agttcgttca gcgtcgcccc ggtggtaagc acgtcgtcca
    11221 ccacggcgat ccgttttccg tcgaggtcga cgtcgcagac gaaagcacga cgcacgttcc
    11281 ttgctcgttc cttccacggc agcgccgcct gcgggggcgt atcggccacc ttgcggcaag
    11341 cagcgggcag caacggcatc ccggtacatg cggcaacttc ccgcgcgatc tcaagcgcct
    11401 ggttgaaccc gcgctcggca agccgtccgc gcgccagagg cattggcacg atagcatcca
    11461 cgtcgcgcgc aacgcgctgc gcgagcagct cgcccaatat tcgcgccagc gccagcctgc
    11521 cgcggtactt gaatgcgtga atcagcgcat ccaccgggaa gcggtaggcg aaaattgctt
    11581 ccacccggtc gaagcggggc ggccgctcga ggcaggtgcc gcacactttg ccgctcgcca
    11641 gaggcagggc gcatacctcg caacagcggc gcggcaacca cggcagatcc gtcaggcatg
    11701 gcgggcacag cggctcgtcc gtgctgtcag ccccgcacag caggcacgat tgcggcagca
    11761 ggatccgcgc aagagataga cagttgttta ataatcccgg gatgaaattt gacaagctgc
    11821 gcgtccggcc cgatgataca agtgtctcaa aacgcagcat tttttctgat cgcatggggg
    11881 aagccgcgtg gatacttcag accttaacga atcccgggtg actgcggctg acaacgcgtc
    11941 cgttgcgcgg cgatgggacc cgcgcgaggt cgagggcctg tacgagctgc cgttcaacga
    12001 cctcattcac cgggcgcatg gcgtgcaccg gcgccatttc gatcccaacg ccgtgcagct
    12061 ttccaccctg ctgtcggtga agaccggcgg ctgtccggag gactgcgcct attgcccgca
    12121 ggctgcccgc tatcaaaccg gcgtcgccaa cgaggaaatg ctttcggtcg agcgggtggt
    12181 agcggcggcg cgactcgcca agcggaacgg cgcgacgcgc ttttgcatgg gcgcggcgtg
    12241 gcgcggcccg aaacagcgcg agctcgagcg ggtgatcgag atggtgagag ccgtgcgcgc
    12301 ggaagggctg gagacctgcg cgacgctggg gctgctcaag aaaggacagg ccgagcagct
    12361 caaggccgca gggctcgact attacaacca caacatcgat acctcccccg agttctacgg
    12421 cgggatcatc acgacgcgca cccaggacga ccgattggag acgctcgagc gagtgcggca
    12481 ggccggcctc cacgtctgct gcggaggcat catcggcatg ggcgaatcgc gccgcacccg
    12541 cgcggccctc atcgcccagc tcgcgagcat ggacccgtac ccggagtcgg tgccgatcaa
    12601 caacctggtg caggtgccgg gcacgccgct gcacgggacg gaaaagctcg accccctcga
    12661 attcgtgcgc accatcgcct gcgcgcgcat caccatgccg agggcgatgg tacggctttc
    12721 cgcgggccgg caggaaatgt cggaagcggt ccaggcgttg tgcttcttcg ccggcgccaa
    12781 ctcgatcttc tacggcgaga agctgctcac taccggcaat cccgacgtcg agcaggaccg
    12841 cgcgctcttc gcgaagcttg gcctgcgccc gcttcaccga tgagttttga gtgttgagct
    12901 ttgcggcgac cgtcaagact gctggacgtt cgaagtgacc aactcgacac tcgacactcg
    12961 acactcgaca ctccagcaag cgcttgcggc ggaactcgga gaactcgaat cccgagacct
    13021 gaaacgccac cggcgcttgc tggaatcgcc acaaggtgcg cgggtcaggg tcgacggccg
    13081 ggattacata gcgttctgca gcaacgatta cctcgggctt gcctcccatc ctgaattggt
    13141 cgaggccgca cgccagggcg cggcccgcta tggcgtcggc gccggcgcgt cccatctgat
    13201 actcgggcat tctgcggcgc atcacgagct ggaacagcgg ctggcggcgt tcgtcggcct
    13261 gccgcgtgcg ttgttgtttt ccaccggcta catggcgaac atcggcgtcg tgtcggcgct
    13321 ggtgggacgg gaggacgcgg tgtttgccga ccgtctgaac catgcgtcac tcaatgacgc
    13381 ggcgctgctc tcgcgcgcgg cgttcaaacg ctacccgcac ctcgacctcg gctcgctcgc
    13441 gcggctgctt gcctcatcgc gcgcgcgccg caagctcatc gtcaccgatg cggtgttcag
    13501 catggacggg gacattgcgc cggtgccggg cctgatcgac ctgtgcgatc gctacgacgc
    13561 ctggttgttg ctcgatgacg cgcacggctt cggcgtgctg gggccggaag gacgcggcgt
    13621 gctcgcgcga ttccgcgcca ggtccccgtg cgtgatctat atggctacgc tgggcaaggc
    13681 cgcgggcgtg ttcggcgcct tcgtagcagg tgaaaccgaa gtggtcgagc tgctcatcca
    13741 acgcgcgcgc gcctatatct atacgaccgc aacgccggcg ctgctcgccc aagccttgat
    13801 gaagagcctg gaactcgtcc gtgcggagga atggcggcgc gagcgcctgc gggctctcgc
    13861 gcagcggctg cgggaatgcc tgcaacccgc gcgctggagg ctcgcgcatt cggatacggc
    13921 gatacagccg ctcgtgatcg gcggcaacga tgaggccgtc gccgtgtcgg aggccctcgc
    13981 gcgccgcggt ctgctggtgc cggcgatccg cccgcccacc gtgcctcagg gcacggcgcg
    14041 cttgcggata tcgctatcag ccgcgcactc cgagcaggat gtcgaaaagc tggccgcagc
    14101 gctgcgtgaa atcgagcgca ccgtgcccgg ttctgagttt cgagttccga gttccgcgct
    14161 caaggccgat ggctctaccc ggtaacacgc aacgcccaac ccgcaacccg aaacggcggc
    14221 acgacatccg ggatgtcgtg ttgctgcatg gctgggggag cgggcccggg atatgggacg
    14281 atttcgcgcg gctgctgaaa ccacgatttc gcgttcacgc tctgccactg cccgggtacg
    14341 gcgcggatac agagcccgct acgcgctcgc tgcgggcgac cgtcgctgcc gttgcgcgcg
    14401 ctgcgccgcg ccgctgcggc gtcgtcggct ggtcccttgg cggcgaagtc gcgcttgcct
    14461 gggcgcgatg cgctcgacac caggtgcgac gcgtcgcgct gatcgcgtcc acgccgtgct
    14521 tcacgagccg gcccggttgg ccgtccgcaa cggctccggc agtgctgcag gaattcgggc
    14581 ggtcgctgct cgctgaccgc gctggcacgc tggcgcgttt caccgaggta caggcgaaag
    14641 gcgatgcgct cgcgaggcgg gttgcctgcg tgctgcaacg attctacgaa caaagcgcat
    14701 cgaccgacgt gctcgtggcg ggcctcgacg tcctggcgag cactgatctg cggcgcgagc
    14761 tgtccagggt gcggcaaccc gcactggtat tgcatggcgc gcgcgatcgc atcgtgccgc
    14821 ctgccgcagg acgccggctt gccgcggcgc ttcccgacgc gcgttttgta ctgctgcggg
    14881 cctgcgccca cgcgccattt ctgtcgaaac cgggggagat ggcggcggcc ctgcgggaat
    14941 tcttcgatga gtgacgcgct cgcgctggac aaacggctcg tgcggcgctc gttcgagcag
    15001 gccgcggcga cctatgacgc ggccgcggtg ctgcaaaacg aggtgtgccg gcgcatgctc
    15061 gggcgactcg attacatcag gctcgcgccg gcgacgatcc ttgacgcagg ctgcggcacc
    15121 ggaaacgccg tcgcgggatt gctcgcgcgc tacccgcggg cgcgtatcgt cgcgatcgat
    15181 ctcgcgctcg ccatggcgcg gcgcgcgcgc gcgcggcatc cgtggtggcg tggctggctg
    15241 gagcggggcg ggccgcggct caccgccgtg tgcggcgaca tcgagcagct gccgcttgcg
    15301 cccggatgcg ccgacctcgt ctggtccaac ctggcggtgc agtggatcaa cgaaccgcgg
    15361 cgggcctttg cggaaatgca ccgcgcgctg gccgcggggg ggctgcttct cttcagcagc
    15421 ttcgggcccg acacgctgaa ggagctgcgg gcgtcattcg agggcctgga ccaccacacc
    15481 cacgtgcacc gtttcatcga cctgcatgac gtcggcgaca tgctggttgc ctgcggtttc
    15541 gccgacccgg tgatggacat ggagatcgtg acgctcactt acgccgacgt gcaggggctc
    15601 atgcgcgatc tcaaggcgat cggcgcgcac aacatgacgt gcgggcgtcc cgcgggcctg
    15661 ggcggcaggt cgctgctcgc cagggtggcg cagaactacg aggcgtttcg ccgcgagggg
    15721 aggttccccg cgaccttcga ggtcgtctac ggccacgcct ggaaaccgca gcctcgaatg
    15781 actgccgacg ggaaaccgat aatccctctc aaacccttgc ggtagggcgg gctaaggatg
    15841 aaggcagaag gcagaaggca gaaggaaaca agacgcgcgg agcgcggggt catccttcat
    15901 ccttcatcct tcatcctttc ccaagtcgct tgtttgggct tattcgccgc accacccaga
    15961 gcgtggccaa gggctacttc gtaactggaa ccgacaccgg cgtcggcaag acgctggtag
    16021 catgcgcatt gcttcgcgcg ctcgcagcgc gaggactcgc ggcagttggc atgaagcccg
    16081 tcgcgagcgg tgccgaactc cgcggcaacg aactcgtgaa caatgatgtc gagcagctga
    16141 tcgcggcgag cagcgttgtc gcaccgcgca gtcacgtcaa tccttattgc tttacgccac
    16201 ccgtcgcgcc gcatatcgca gcgcagcagg cgggagtcac gatcatgctc gaccggatcg
    16261 agcaaagctt ccacgcgctc gccgcgcgcg cgcaggtggt cgtggtggag ggtgtgggcg
    16321 gttttcgcgt gccgcttgga gcgggcagcg atacctcaca actggccgtg cggctcgccc
    16381 tgcccgtggt gctggtggtc ggcgcgcggc ttggctgcct gaatcacgcc ttgttgacgg
    16441 tggaagccat taccactcgc gggctgaggc tcgccggttg ggtcgccaat caccttgatc
    16501 cgcagatggc cgctgcagac gagaacgtac tggcgttgca ggcgcttatc gccgcgcctt
    16561 tgctcgcccg gatcgggtac gcgtcgaacc cggaacccgc gttgatcgca acctcgctgg
    16621 acatcggcaa actgctgtga cgcgcgcgct gcaaatcgtt gcgaatgcag cgttttgccc
    16681 gccctgtcgg ttgcctagcg agggcggcct atgatacatt ccaccggctt caggcggatg
    16741 tgggcggggc acacaatggc cgcaggaatg caatgcagcg agacggcgca cgcggccgag
    16801 ttttcgtggg tcgccaggcg caataactcg ctcagttcca gcggcaggca tctcgttttt
    16861 gcgttcctcc tcgtcgtttc ggtgggcatc gcggcagctt ttgcgtattt cggcgcatgg
    16921 ctggtcctgc cgtttgccgg gctggaaatg ctggtcctct acctggcatt ccgctacatc
    16981 gagcgccacg ccgcggatta cgagctggtt gcgatcgacg gtgacagggt caagatcgaa
    17041 atctgcgaag cgggacgcgc gcgcagccat gagctcaatc gctactgggc gcagttggtg
    17101 agccgcgatg gcgggcggtt ggcgctgcgc tcccacggcc gcgaggtcga gatcggccgg
    17161 cacatgaacg aggatgagag gctcgcgctc gcgcgcgagc tcggacgcca gctgcggggc
    17221 ggcgtggtgc actgaaaacc gcagcggccg cgcggccacg acaacacatc tggattcttg
    17281 ggtgaggaga gcatgatcag caaggggtta cgatcgtttg cggccgcact agcgtggctc
    17341 gccgcggaaa cggcgcttgc caaccgctac aatctgcagg agccgcaatc catcatcgcg
    17401 cgcgagatct acgacctgca cacgctgatc atgtggatca tcgtcggtat cttcgtggtc
    17461 gtgttcggct cgatgaccta cgccatcgtc aagcaccgca aatccgtcgg ccacaaggcc
    17521 gagcagttcc acgagaacac cacggtcgag atcatctgga ccatcattcc cttcttcatc
    17581 ctgatcggca tggcctaccc ggcgacccgg accgtcatcg cgatgaagga cacctccggc
    17641 tcggacatca cgatcaaggc gaccggctat cagtggaaat ggggctacga ctacctgcag
    17701 gaaggcatca gcttctacag caacctcgcc acgccgcgcg agcagatcga gggaagagcc
    17761 cccaagggcg agaactacct ggtcgaggtg gacaacccgg tggtggtgcc ggtcggcaag
    17821 aaggtccgca tcatcgccac ggccaacgac gtgatccact cctggtgggt gccggcgctg
    17881 ggcatcaagc aggacgcgat cccgggcttc gtgcgcgaca cctggttcaa ggcggacagc
    17941 cccggcatct accgcgggca gtgcgcggaa ttgtgcggca aggaccatgg cttcatgccg
    18001 atcgtggtgg aggcagtcac gcccgagagg tacgcctcat gggttgccga gcagaagaaa
    18061 aaaatggccg cggcggcgcc ggagcccggg aagcaatgga cgctcgacga gctcaaggcc
    18121 cacggcgaga agatctacgc cggcaactgc gtcgcctgcc accagccgac cggcatgggc
    18181 gtgccgggca cgttcccggc gctctccggc tcgaaggtcg tgaacgggcc gaaggaaggc
    18241 cagatccagg tccttctcaa cggcgtcgtc aaggacggca agccgaccgc gatggtttcc
    18301 ttcaagcacc tctcggacgt ggacatcgcg gcggtgatca cctacacgag gaacaactgg
    18361 gccaacaaga ccggagaaat ggtcgccccg gctgatgtca aggctgcgcg gaagtagacg
    18421 gcacccgaac ggtttagcgg agaacgaacc ttatggaagc tgcacacgca cacaacgtcc
    18481 atggtcacgg cgagcatggt caccattaca cggggatcat gcgctggatc accacgacca
    18541 accacaagga catcggcacg ctctacctgt ggttcgcgct caccatgttc ttcgtgggcg
    18601 gcgtgcttgc gctgatgctt cgcgccgagc tgttcgagcc cggcatgcag ttctggcgtc
    18661 ccgagttctt caaccagctc accaccatgc acgggctcat catggtgttc ggcgcgatca
    18721 tgccggcgtt cgtcggtttc gccaactggc aggtgccgat gatgatcggc gcgcccgaca
    18781 tggcgttcgc gcgcatgaac aactggagct tctggctgct gccggtcggg gcgaccctgc
    18841 tggtcgcttc cttcttcgtg ccgggcggcg cgaccgccgc gggctggacg ctgtatgcgc
    18901 cgctctcgac gcagatgggg ccggggatgg acctcgcgat cttcgcgctg cacatcatgg
    18961 gcgcctcctc gatcatgggc tcgatcaaca tcatcaccac catcctcaac atgcgcgcgc
    19021 ccgggatgac gctcatgaag atgccactgt tcgtctggac ctggctgatc accgcctatc
    19081 tgctgatcgc ggtgatgccg gtgctcgcgg gcgcggtcac gatggtgctc accgaccgcc
    19141 acttcggcac ccacttcttc aacgcggcgg ggggcggcga tccggtgctg ttccagcacg
    19201 tgttctggtt cttcggtcat cccgaggtct acatcatgat cctgccgggc ttcggcatcg
    19261 tctcgcagat cgtgccgacg ttcgcgagga agccgctgtt cggctattcc tcgatggtct
    19321 acgcgaccgc ctcgattgcg atcctctcct tcatcgtgtg ggcgcaccac atgtttacgg
    19381 tgggcatgcc cgcggcggcg cagctcttct tcatgtacgc gaccatgctg atcgcggtgc
    19441 cgaccggcgt gaaagtgttc aactggatcg cgaccatgtg gaagggggcg ctgaccttcg
    19501 agacgccgat gctgttcgcg tgcggattca tcttcgtgtt ctcgatgggc ggattcaccg
    19561 gcctggtact ggcgatcacc cccgtggaca tccagctgca ggacacctat tacgtggtcg
    19621 cgcacttcca ctacgtgctg gtcgcgggct cgctgttcgc cctcttcggc ggcacctatt
    19681 actggctgcc gaagtggatc ggccgcatgt acgacgagac gctcggcaag tggcacttct
    19741 ggctgtcgat gatcttcttc aacgtcacct tcttcccgat gcacttcaca gggctcgcgg
    19801 ggctgccgcg gcgggtgccg gactacaacc tgcagttcgc cgacttcaac atgatctcga
    19861 ccatcggcgc gttcggcttc gggttgtccc agctcatctt cgtgtacgtg ctgtggaagt
    19921 gcatcagcgg cgcgggagag aaggtgagcg acagggtatg ggaaggcgcg cacggcctgg
    19981 agtggacgct gccttcgccg ccgccgtacc acaccttcga gaccccgccg gtggtcaagt
    20041 agcgtcatga gcggcggagc agggatcggt ggaacgtgga gcaggggccc gcttgcggga
    20101 tgcgaccgtg aagccgatcg aaggcgcgtc gtgctgatgc cgatcccatg cgcgtctccg
    20161 gggtcagatc cccagagggg cccctcttcc gccctgcgac gcgccccttt ggggatgtga
    20221 cccgcgaagg acgcgccgat gcgatggcct aggcattgcg agagcatgat tgtggggatg
    20281 tcatgaccag caacctcaag accgcgttga tcctggcctc catcgcggcc gcgttcttca
    20341 tcggggtcgt gatccggcac tggctatggt gagccactcg cacgccaacc agcagacgct
    20401 ggtcaagctt ctggtcgtcg ccgtcctgat gttcggcttc ggcttcgcat tagtgccgtt
    20461 ctaccgcgcg atctgcgatg cgctgggcct gaacagcgtg gtcaaggccg acgccgtggc
    20521 gaatacccag gtggataccg ggcggtccct cacggtcgag ttcgacacca acctccgcag
    20581 caacctgccg tggaccttca ccccgctcga aaaaagcgtg cgcatccatc ccggcgcgct
    20641 gacgcaggtc actttcgaga ttcgcaaccg ttccgaccac ccggtcacgg gacaggcgat
    20701 cccgagcttc ggaccgcagc tggcgggccg ctatttcaag aagctcgagt gcttctgctt
    20761 cacccagcag acgctcgcgc cgggcgaggt caggcgcatg ccggtcgtgt tcgtggtcga
    20821 gccgggcctc ccgcgggacg tcaataccgt gacgctctcc tacaccttct tcgaggtgga
    20881 gggcaagacc gggaagggca gttgatgctg atggcggaac aggcgcaacc cccgcgcaag
    20941 gcgacgccgc tgcaggtcgc aaaggccgtg ctgtccgcgt tcatcggcat ccgcaagcgc
    21001 gcggcgcatg agcaggacgc ggtgacgctg tcgccgctgc aggtgatcgt cgcgggcgtg
    21061 atcgccgcgg cggttttcgt attgagcctg gtgatgctgg tgcgttatat caccagttga
    21121 aagggagaga ccaagatgtt gaatcaatcg gcgcgctatt tcataccgga tccttccacc
    21181 tggccgatca ccggatccat cgcgctgctg tgcatggcat cgggagccgc ctcctggttc
    21241 aacggctggt ctcccggcaa gttcatcctg ctcgcaggcg tggccattct tgcctacatg
    21301 ctggtcggct ggttccacac cgtttcgcac gagagcgagg gcggcaagta caacaagcag
    21361 gtggacgtct cgttccgctg gggcatgagc tggttcatct tctccgaggt catgttcttc
    21421 ggggcgttct tcggggcgct gttctacatg cgggtgctgt cggtgccttg gctgggcgat
    21481 ttcgagcaca agctgatctg gccggacttc accgccacct ggccggccac gggccccggg
    21541 ttgaaagacc cgttcacgcc aatggcggca tggggcatcc cggcggtcaa cacgctgctg
    21601 ctgctctctt ccggcgtgac cgtgacgctc gcgcactggg cgctgatccg caatgaccgt
    21661 accaagctca tcgcgtggat gttcgcgacc atcggcctgg gcgtgatgtt cctcgccttc
    21721 cagatctacg aatacggaca cgcctactcc gaactgaatc tcaagctcac caccggcgcg
    21781 tacggttcga ctttcttcat gctgaccggc ttccacggct tccacgtcac catcggctgc
    21841 atcatgctga cgacgatcct gttccgctgc gtcgccgggc atttccggcc cgatcaccac
    21901 ttcggcttcg agggcgtggc gtggtactgg cacttcgtgg acgtggtgtg gctggggctc
    21961 tttatcttcg tctactggct ataagccgat aacacaacaa gaaagcacaa cacacgacaa
    22021 ggcgcaccaa caagaagggg ggaacccgtc agtaggggag gggagcaggc gccgcgccac
    22081 aagtgcggcg tttttttgga ttacgccgaa aatctcgacg caaagacgca aagacgcaaa
    22141 gggtgcagaa taatttaggg tttctttgcg cccttgcgtc aaggttctca ctcgagcttg
    22201 ccggggatga gcccgaagta gaatccgagc atcagcagcg cgaacagcgc cacggacagc
    22261 gccacccgca ccgtaagcgc cttgaccgtg cgctcgccct ggccccggtc cttgaccatg
    22321 aagtagagcg ccgaggcgag gctgccgacg atgaagacga ggaacaggat gacgacgaat
    22381 ttcatggaca ggcggacggg caattgaatt tgtctcaagc atataccctt accaccttgt
    22441 ccgcccgtta ccgttttcag ccccgtctta ttaccacgct cgccgccgcc ggcggcatcg
    22501 tgctgacgct ggcgcttgcc aactggcagc tcggccgcgc gcatgagaag gaagcgctgg
    22561 gccggcggct tgaggccttg gcgaaggatc ctccggtgtg gctgccggcg gccgaggtga
    22621 gggcggaaga cgtggaatgg cggcgggtga cggcgcgcgg ccgcttcgag cccaagcacg
    22681 cggtgttcat cgacaaccgc gtgcgccacg gtgtcgccgg ctaccacgtg gtgatgccgc
    22741 tcgccatcgg cggcggcagt cgctacgtgc tggtgaaccg cggctgggtc acggggtccc
    22801 ccgaccgcgc caggctgccc caggtgaaga cgccggaggg gacggtggaa attaccggcc
    22861 tcgcggtcac gccgagccgg cgctttctgg aactctccac ccacgtcgcg gaaggcaacg
    22921 tctggcagaa cctgacgctc gagcgctacc ggcaggcggt gccgatcgcg ttgcagccgg
    22981 tggtgatcca gcaggagagc ccgctcgacg acggcctcgt gcgcgagtgg gatcccccga
    23041 acctcggcgt cgagaagcat tacggctatg cgttccagtg gctcgcgctc gccctcacga
    23101 tactcgtgtt ctacctcgtg acccatgtcc gacgccgctc cccggaagag gctcactagc
    23161 ctgtggctga tcatcgcggt ggcggcggcg ccggtcgtgg cctcgtacct gctctattat
    23221 ttctggccgc ccgaacgcac cgtgaaccat ggcgaactcg tcgagccgcg gccgctgccg
    23281 gaccctgagc tggcgctcgc cgacgggagg ccgttccggc tctcgcaatt gaaggggaaa
    23341 tgggttctga taaccgtgga ctcgggacgc tgcgatgctc actgcgagag aaagctcacc
    23401 tacatgcggc agcttcggct cacccagggc aaggagatgg aacgcgtgga gcgggcgtgg
    23461 ctcatcagcg atgggattgc gccgagaccc gagacggtcg cgccgtacca cggaacctgg
    23521 ctcgtgcgcg ccgccggaac cgaattgctc aaagccttcc cggcgcaggg tgcggcctcc
    23581 gaccatatct acatcgtgga tcccctcggt aatctcatga tgcgctttcc acgcgatccc
    23641 gatccccggc tcatgatcaa ggacctcgcg cggctgctca gggcgtcgcg catcggctga
    23701 tgcgcggtgt tttgccggcg gcgcggggga tgctaaactt tgccgtctcg aaacgaaaac
    23761 accacgactt agatgtcctc cagcctcgcg cgacaaccgc tcgcctcacg gttcagccag
    23821 ttttaccagc tcaccaagcc gcgcgtggtg tcgctcatcg tcttcaccgc ggtgatcggc
    23881 atgttcctcg ccacccctga catggtgccg ctgcaggcgc tcgtcgcggg cactctcggc
    23941 atcgcgctca ccgcgggcgc ggcggcggcg gtgaactgcc
//
LOCUS       DBNGWZ010000447         1741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_308288, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000447 DBNGWZ010000000
VERSION     DBNGWZ010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_308288"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="AC16CX_13700"
     CDS             complement(<1..941)
                     /locus_tag="AC16CX_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3013600.1"
                     /translation="MKPVERIRCTLARQPIDQLPVGPYLANWTASFAGIPLSSYCTDA
                     RQMAAVQLAAWEHIGQDVIFPDADNYYLAEGFGCTSCFYEDNFPTLARPALEHPQQVF
                     ELEIPDPYRDGRMPVYLEATQRIAEQVGDQAVIRVPGTGPFAVASYLIGIEPFLVEVG
                     LIQKGRDLSNAAAVERMLDLAAEAVSRFGLAQLRAGAHILQCGDSLASGSVISPATFR
                     RFVLPRHQKIFSTWKQAGAITVLHICGNNTRMLDLLAETGADIVAIDSLVDLRLAKET
                     IGDRVCLIGNIDPVAVMLQGSAKRVEAVAQECVNAAAA"
     gene            complement(955..1719)
                     /locus_tag="AC16CX_13705"
     CDS             complement(955..1719)
                     /locus_tag="AC16CX_13705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013601.1"
                     /translation="MAETKHFLADKVVLITGAARNLGAVVATEFASHGARLAIVDRRF
                     TDEAEERAEMLNRGPGRAAHFVGDVSDATQITAVIKRVGDTFGPVDVLINNAGPFAID
                     PFLKLRVEDWDFVMNSNVRAIYLFAQQVAPGMNERGWGRILNIAAGSAFLRNHSVYGL
                     AKAAVMFLTETLALELKPGVTVNAIAPGQIAESAPDISAYDPTFVERAIRITPTGRLV
                     ARADIARMMVELCSPAFDMVTGMTLPMDGGARLPRF"
BASE COUNT          328 a          560 c          552 g          301 t
ORIGIN      
        1 gccgcggcgg cgttgacgca ctcctgcgca accgcctcca ctcgcttggc gctcccctgg
       61 agcatcaccg ccaccggatc aatgttgccg atgagacaca cacggtcgcc aattgtctct
      121 ttggccaagc gcaaatccac cagtgagtca attgcgacga tgtctgcacc tgtctcggcg
      181 agcaggtcga gcatacgggt gttgttccca cagatgtgca agacggtgat ggccccggcc
      241 tgcttccagg tgctgaagat tttctggtgc cggggaagga cgaagcggcg aaacgtggcc
      301 ggggagatga cgctaccgga ggcgagtgaa tcgccgcact gcaggatgtg cgcgccagcg
      361 cgcaactggg ccagcccgaa ccggctgacc gcctcggccg ccagatccaa catgcgctcg
      421 accgccgctg cgttcgacag gtcccggccc ttttggatca gcccgacttc gaccagaaat
      481 ggctctatgc cgatgagata cgaggcgacg gcgaacggtc cggtgcctgg cacacggatg
      541 acggcctgat ctcccacctg ctctgcgata cgttgcgtgg cttcgagata gactggcatc
      601 cgcccatccc gataggggtc agggatttcc aattcaaaga cctgctgcgg gtgttccagc
      661 gccggccgcg cgagcgttgg gaaattgtct tcataaaagc atgaggtgca gccaaacccc
      721 tcggccagat agtagttgtc ggcgtccggg aaaatcacgt cctgcccgat gtgctcccag
      781 gcggcgagtt gcacggcggc catctgccgc gcgtcggtgc agtaggacga gaggggaatc
      841 ccggcgaacg aggcggtcca gttcgccaga taggggccga ctggcaactg gtcaatcggc
      901 tggcgcgcaa gcgtgcagcg gatccgttcg acgggcttca tcgccgggtt ctgctcaaaa
      961 gcgcggcagg cgcgcgccgc cgtccatcgg aagggtcatc ccggtcacca tgtcgaaggc
     1021 tggggagcag agctcgacca tcatccgggc gatatccgcg cgcgcgacca ggcgtccggt
     1081 cggcgtaatg cggatcgcgc gctccacaaa cgtaggatca taggcgctga tgtccggcgc
     1141 gctttcggca atctgccccg gcgcgatggc attgaccgtc acccccggct tgagttccag
     1201 cgccagggtc tcggtcagga acatcaccgc cgccttagcc aggccataga ccgaatgatt
     1261 gcgcaggaaa gcggaaccgg ccgcgatgtt gaggatgcgc ccccagccgc gctcgttcat
     1321 gccgggcgcc acctgttggg cgaacaggta gatcgcgcgc acgttggagt tcatcacgaa
     1381 gtcccaatcc tcgacgcgca gcttaagaaa cggatcgatg gcgaaagggc cagcgttgtt
     1441 gatcagcacg tcaacgggcc cgaaggtgtc ccccacccgt ttgatcaccg cggtgatctg
     1501 ggtcgcgtcc gaaacgtcgc cgacgaagtg ggcagcccgg cccggtccac ggttcagcat
     1561 ttcggcccgc tcctccgcct cgtcggtgaa gcggcgatcg acgatcgcca gacgggcacc
     1621 gtgagaggca aactcggtcg cgacgacggc ccccaggttg cgcgccgcgc cggtgatcag
     1681 taccacttta tctgccaaaa agtgtttcgt ctcagccata gctcacgaat cctccttcca
     1741 c
//
LOCUS       DBNGWZ010000448         7595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2701215, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000448 DBNGWZ010000000
VERSION     DBNGWZ010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2701215"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /gene="recR"
                     /locus_tag="AC16CX_13710"
     CDS             <1..127
                     /gene="recR"
                     /locus_tag="AC16CX_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671285.1"
                     /note="involved in a recombinational process of DNA
                     repair, independent of the recBC complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3013602.1"
                     /translation="EALRARGLKVTRIARGLPVGGELEHVDSGTLAQAVLERREA"
     gene            206..1048
                     /gene="rluB"
                     /locus_tag="AC16CX_13715"
     CDS             206..1048
                     /gene="rluB"
                     /locus_tag="AC16CX_13715"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF007976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HIR3013603.1"
                     /translation="MKIIRHAAGDVATAKLQKVLAQAGLGSRREMEQWISAGRVTVNG
                     VTARLGARVSASDVVKLDGRPVQAMASSRSPQVLLYHKPEGEIVSRDDPAGRPSVFDK
                     LPPLRGAKWMAVGRLDFNSCGLLVFTTSGELANRMMHPGQELEREYAVRVLGKLSDEQ
                     IDRLRSGIRLEDGVARCDSVASEGGEGANRWYRIVLKEGRNRIVRRLFEALGLRVSRL
                     LRVRFGPIALPPRLRRGRFQELSPTEIRQLLISLGMPESAPLPRVARGLAGTHRRRRP
                     YRAR"
     gene            complement(951..1841)
                     /gene="prmB"
                     /locus_tag="AC16CX_13720"
     CDS             complement(951..1841)
                     /gene="prmB"
                     /locus_tag="AC16CX_13720"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR3013604.1"
                     /translation="MGGLVRSGTRRFRAAGLAFGHGTTNARDEAAYLTLHALKLPLDA
                     ARGAFARRVGPRAARHVLNLFERRIRERKPAAYLTREAWLGGLRFYVDERVIVPRSHI
                     AGLLLDRLDPWISDPAGVRTALDLCTGSGCLAILLAKSFLRSRIDAADVSSEALAVAR
                     VNVRRYRLGRRIRLVESDVFSALRGKRYDLIVCNPPYVTTRSMRRLPREYRHEPRAAL
                     AGGKDGLVIVQRILRAASRHLYPGGHLVVEVGAGRNAVERACPRLQFTWLDTDGGGDV
                     FLLTRGQLEAAAPIPASRAR"
     gene            complement(1877..3034)
                     /locus_tag="AC16CX_13725"
     CDS             complement(1877..3034)
                     /locus_tag="AC16CX_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR3013605.1"
                     /translation="MFVTPLLKLMADKQASDLFFTAGAPIQIKINGVVMPINNQLLDA
                     EQAKKICYELMSEAQIKEFEATHEMNFAQSGGEIGNFRVNIFRQKNTVAMVIRFVKPD
                     VPVFDSLGLPPILKEVIMEKLGLILVVGATGSGKSTTMASMIDYRNNIKTGHILTVED
                     PVEFMFRHKKSIVNQREVRVDTQSYGNALVSAMREAPDLIMIGEIRDRPTLENALLFA
                     QTGHLCMSTLHANNSYNALNRIINFFPRESREALLADLAIALRAVISQRLVRTVDGKR
                     VPAVEILLNTKHIQELIKSGEISQIKDALEQSLAPGSQSFEQALFNMITSGKVAIEEA
                     MVHSDSPTNLHWLLNNAPKGTLAAAAQSAGVTKQPAPDDISSIKLNLDALG"
     gene            complement(3189..4010)
                     /gene="dapD"
                     /locus_tag="AC16CX_13730"
     CDS             complement(3189..4010)
                     /gene="dapD"
                     /locus_tag="AC16CX_13730"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3013606.1"
                     /translation="MTELQSIIDQAWERRAEINPANRDAELKGAVEQTIDLLDRGKLR
                     VAEKIRGEWVTHQWVKKAVLLSFRLQDNFVMKDGYTRYYDKVAAKFSDYTPQAFQQGG
                     FRVVPPAAVRKGVYIGKNVVLMPSFVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVVVEEGSVISMGVFIGASTRIYHRQ
                     TGEILYGRVPALSVVVPGSLPAADGRCSLYCAVIVKQVDARTRAKTSINELLRSE"
     gene            complement(4072..5265)
                     /gene="dapC"
                     /locus_tag="AC16CX_13735"
     CDS             complement(4072..5265)
                     /gene="dapC"
                     /locus_tag="AC16CX_13735"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 -
                     succinyldiaminopimelate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR3013607.1"
                     /translation="MNPDLELLQTYPFQKLRALLQGVEPCPGRDPIAMHIGEPKHPTP
                     AFIQQALIEHLDGLASYPQTLGGQTLRSAIAGWLIRRYGLESVDPETEIIPVNGSREA
                     LFAIAQAVVDASRTEPAVVCPNPFYQIYEGAALLAGARPHYLNNLPLRGYACDFDELP
                     EKTWKRVQLVYVCSPGNPTGHVMSLEEWKRLFALSDRYGFIIASDECYSEIYFNEARP
                     PLGSLQAARQLGRQGYPRLVMFSSLSKRSNVPGMRSGFVAGDAAVLKKFLLYRTYQGC
                     AMGPAVQAASVAAWKDEDHVAENRRLYREKFAAALEILRPVLDVEMPDAAFYLWPETP
                     LPDPEFARRLHQEQAVSVLPGSYLAREAEGVNPGANRVRIALVSSHAECVEAARRIRA
                     FVESL"
     gene            complement(5357..5779)
                     /gene="queF"
                     /locus_tag="AC16CX_13740"
     CDS             complement(5357..5779)
                     /gene="queF"
                     /locus_tag="AC16CX_13740"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR3013608.1"
                     /translation="MPKTPSRRLATFANPHPARDYRIHMEIPEFTCLCPRTGQPDFAT
                     LLLDYVPDRRCVELKSLKLYVWSYRDEGAFHEAVTNRILDDLVRATRPRYMRLTARFN
                     VRGGIFTTVVAEHVKPGWERHTVVDLAGLPEISSARSG"
     gene            5857..>7595
                     /locus_tag="AC16CX_13745"
     CDS             5857..>7595
                     /locus_tag="AC16CX_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3013609.1"
                     /translation="MRLKQINLAGFKSFVDPTHIPVPGQLSGIVGPNGCGKSNVIDAV
                     RWVLGETSAKHLRGDTMQDILFNGSGQRQPVNRASVELIFDNSLGRAGGQWASYAEIS
                     VKRVLQRDGEATSYYINNVHVRRRDVADIFLGTGLGSRAYAIIEQGMISRVIEAKPEE
                     LRIFLEEAAGVSKYRERRRETEYRLKDTRENLLRVDDVRMELDKQLEHLQSQAEVAAR
                     YHELQARLRTVQNLLWLLRKQEAGAQRARHLKEIERLTLELEAETARLRETEKRLEDM
                     RAQHYRGSDEAHAAQGAFYEANAETARLEQELSHLRENRQRVERELSDLHAQVGRGRE
                     QLAAAQASLAELRNERDRAAESVMAREAAVGSEGDKLPVAEEAFRATRNRYDELQRAL
                     ALSEQAMGVEQTKRAHAEQMLEQLALRAQRLREEDTALQPADAPRLQRLRGDIAALDE
                     ELHRLHAAMESEEERLPGAEQAMRERGVAQELAGQRLAGLEAQRQALTRLQERLARGA
                     NTKDWLETHGLGKAPRLWQQIRIESGWEDALEAVLHQRLNAIALERMEQSGRWFNEPP
                     PGRITVFAPAREAA"
BASE COUNT         1340 a         2386 c         2570 g         1299 t
ORIGIN      
        1 cgaagccctg cgggcccgcg gcctgaaagt cacgcgcatc gcgcgcggac tgccggtcgg
       61 gggcgagctc gagcacgtgg acagcggaac gctggcccag gcggtgctcg agcggcgcga
      121 ggcctgagga ttttgccggc tcctggccgg taaaatcctt gcaagggatc gtctctggga
      181 atgcaacgaa agcgacctcc ctgtcatgaa aatcatccgt cacgctgccg gggacgttgc
      241 aactgccaag ctgcaaaagg tgctggcgca ggccggattg ggctcgcgtc gcgaaatgga
      301 gcagtggata agcgcaggcc gcgtgacggt gaatggcgtg acggcgcggc tcggcgcacg
      361 cgtcagcgca agcgacgtgg tgaagctcga cgggcgtccc gtgcaggcga tggcgtcctc
      421 gcgctccccg caggtgctgc tctatcacaa gcccgagggg gagatcgtca gccgcgatga
      481 ccccgccggc cgtcccagcg tgtttgacaa gctgccgccg ctgcgcggcg cgaaatggat
      541 ggcggtcggc cgtctcgatt tcaacagctg cgggctgctg gtgttcacca cctcgggtga
      601 gctcgccaac cgcatgatgc atccgggcca ggagctggag cgggaatacg cggtgcgcgt
      661 gctcggcaag ctctccgacg agcagatcga caggctgcgc tccggcatcc ggctggaaga
      721 tggcgtggcg cgctgcgatt ccgtcgcgtc cgagggcggc gagggcgcca atcgctggta
      781 ccgtatcgtg ctcaaggaag gccgcaaccg catcgtgcgc cgactgttcg aggccctggg
      841 attgagagtc agccgactgt tgcgagtgcg gttcggcccg atcgcgctcc cgccgcggct
      901 gcggcgcggg cggttccagg agctttcccc cacggagatc cgccagctgc ttatctcgct
      961 cgggatgccg gaatcggcgc cgctgcctcg agttgcccgc gggttagcag gaacacatcg
     1021 ccgccgccgt ccgtatcgag ccaggtgaac tgcagccgcg ggcaggccct ctctaccgcg
     1081 ttgcgaccgg cgccgacctc taccaccaga tggccgcctg gatagaggtg ccggctggca
     1141 gcgcgaagta tgcgctgcac gatgacgagg ccgtccttgc cgccggcgag cgccgcgcgg
     1201 ggctcgtgcc ggtactcgcg tggcagccgc cgcatgctgc gtgtcgtcac gtacggcgga
     1261 ttgcagacga tgaggtcgta acgcttcccg cgcagcgcgg aaaacacatc ggattcaacc
     1321 aggcggatgc gacggcccag ccggtacctc cggacattga cccgcgccac ggcaagcgct
     1381 tccgaagaga catcggcggc atcgatcctc gaacgcagaa agcttttcgc cagcaggatg
     1441 gcgaggcagc ccgagccggt gcatagatcg agcgccgtcc tgaccccggc ggggtcggaa
     1501 atccagggat cgaggcggtc gagcagcaag ccggcgatgt gggagcgggg gacgatgact
     1561 cgctcgtcca cgtagaagcg cagaccgccg agccaggctt cgcgcgtcag gtatgcggcc
     1621 ggtttgcgct cacgaatgcg ccgctcgaaa agattcaaaa cgtgccgcgc cgcccgcggg
     1681 ccgacgcgcc gggcgaaagc gcccctcgct gcatcgagcg gcagcttgag cgcatgcagg
     1741 gtgagataag ccgcttcgtc gcgagcgttg gtggtgccgt gtccgaaagc gaggccggcg
     1801 gcgcgaaagc ggcgcgtgcc gctgcgcacc agtccgccca cggtccacgg gccggccgct
     1861 cgtggttggc gtgcgctcaa ccgagggcgt cgagattcag cttgatgctg gaaatgtcgt
     1921 caggggccgg ctgtttggtg acgccggcag attgcgccgc cgcggccagc gtgcccttcg
     1981 gggcgttgtt gagcagccag tgcaggttgg tgggtgagtc ggagtgcacc atggcttcct
     2041 cgatcgccac cttgcccgag gtgatcatat tgaacaaggc ctgctcgaac gactgcgatc
     2101 cgggcgcgag gctctgctcg agggcgtcct tgatctggct gatctcgccg ctcttgatca
     2161 gttcctggat gtgcttggtg ttgagcagga tctcgacggc aggcacgcgc ttgccgtcca
     2221 cggtgcgcac caggcgctgg gaaatgaccg cccgcagcgc gatcgcgagg tccgcgagca
     2281 gcgcttcgcg cgactcgcgc gggaagaagt tgatgatgcg gttcagcgcg ttgtagctgt
     2341 tgttggcgtg cagggtggac atgcacaggt gtccggtctg ggcgaacagc agcgcattct
     2401 ccagcgtcgg gcggtcgcgg atctcgccga tcatgatcag gtcgggggcc tcgcgcatcg
     2461 cgctgaccag cgcgttcccg tagctctgcg tgtcgacgcg aacctcgcgc tggttgacga
     2521 tcgatttctt gtgcctgaac atgaactcga ccggatcctc gacggtgagg atgtggccgg
     2581 tcttgatgtt gttgcggtaa tcgatcatag aggccatggt ggtggacttg ccggacccgg
     2641 tcgcgcccac cacgaggatg aggccgagct tttccatgat cacttccttc aggatcggcg
     2701 gcaacccgag ggaatcgaac accggcacgt cgggcttgac gaagcggatc accatcgcca
     2761 cggtgttttt ctgccggaag atgttcacgc ggaagttgcc gatctcgccg ccgctctgcg
     2821 cgaaattcat ctcgtgcgtg gcctcgaatt ccttgatttg cgcctcgctc atcagctcgt
     2881 agcagatctt cttcgcctgc tcggcgtcga gcagctggtt gttgatcggc atcaccacgc
     2941 cgttgatctt gatctggatc ggcgccccgg ccgtgaaaaa caggtcggag gcctgcttgt
     3001 cggccatcag cttcaacagt ggtgtcacga acatgaccgt ctccttcccc cgcgtgtatg
     3061 ctctcgtcct gaccgcgcgt tgagcggtgc cgggcatccg ctccaacgcg cggagtcaaa
     3121 ccggcatttt atgtccagtt taatgataat tgtgccaatt aatggccggg ttgcctagcg
     3181 gcgcggagtt attcgctgcg cagcagctcg ttgatgctgg tcttggcgcg ggtcctggcg
     3241 tccacctgct tgacgatgac cgcgcaatat aggctgcagc gcccgtcggc ggcgggcagg
     3301 ctgcctggca ccaccaccga gagcgccggc acgcgcccat agaggatctc cccggtctgg
     3361 cggtggtaga tccgggtgct cgcgccgatg aacacgccca tcgaaatgac cgagccttcc
     3421 tcgacgacga cgccctcgac gatctcggaa cgcgccccga tgaagcagtt gtcctcgatg
     3481 atggtgggat tggcctgcaa cggctcgagc acgcccccga tgcccacacc gcccgagaga
     3541 tgcacgtttt tcccgatctg ggcgcaggag ccgacggtcg cccaggtgtc caccatggtc
     3601 ccttcatcga cataggcgcc gatgtttacg aagctgggca tcagcaccac gttcttgccg
     3661 atgtagacgc ccttgcgcac ggctgccggc ggcaccaccc ggaacccgcc ttgctggaac
     3721 gcctgcggcg tgtaatcgga aaatttcgcc gcgaccttgt cgtagtagcg ggtataaccg
     3781 tccttcatca cgaagttgtc ctgcagccgg aacgacagca gcaccgcctt cttgacccat
     3841 tgatgagtga cccactcgcc ccggattttc tccgcgacgc gcagcttccc ccggtcgagc
     3901 aggtctatcg tttgttccac cgcgcccttc agttccgcgt cgcgattggc cgggttgatt
     3961 tcggcgcggc gttcccacgc ctgatcgatg atggattgca gctcggtcat gtgggcgatt
     4021 ctacctccag ttaaaacgga ccgctcacgt gcttgccgct caaaccgtgc atcagaggga
     4081 ttccacgaag gcgcgtatgc gccgcgccgc ctcgacgcat tcggcatggg aagagaccag
     4141 cgcgatgcgc acgcggtttg cgccggggtt cacaccttct gcttcgcgcg cgaggtagct
     4201 gccgggcagt accgatactg cttgttcctg atggaggcgg cgggcgaatt ccgggtccgg
     4261 caatggggtt tccggccaca ggtagaaggc ggcatcgggc atctcgacat cgagcacggg
     4321 gcgtaatatc tcgagcgccg cggcaaactt ctcgcggtac aggcggcggt tttcggcgac
     4381 gtggtcctcg tctttccagg ccgccacgct ggcggcctgc accgcgggtc ccatggcgca
     4441 gccttggtag gtgcggtaga gcaggaactt cttgagtacg gcggcgtcgc ccgcgacgaa
     4501 ccccgagcgc atgcccggca cgttggagcg cttggacagg ctggaaaaca tcaccagccg
     4561 gggataaccc tggcgtccga gctgccgcgc cgcctgcaac gagccgagcg gcggccgcgc
     4621 ctcgttgaaa tagatctcgg aatagcattc atcggaggcg atgatgaagc cgtagcggtc
     4681 ggagagcgcg aacagccgct tccattcttc gagcgacatg acatgaccgg tcgggttgcc
     4741 cggcgagcag acataaacga gctggacgcg cttccacgtc ttctccggca gctcgtcgaa
     4801 atcacaagca tagcctcgca gcggcagatt gttgagatag tgcggcctgg cccctgcgag
     4861 cagcgcggct ccctcgtata tctggtagaa gggattggga cagacgaccg ccggctccgt
     4921 acgggacgcg tcgaccacag cttgggcgat cgcgaacagc gcctcgcggc tgccgttgac
     4981 cgggatgatt tcggtctcgg gatccaccga ttccagcccg taacgccgta tcagccatcc
     5041 ggcgatggcc gatcgcagcg tctggccgcc cagcgtctgg ggatagctcg ccagtccgtc
     5101 gagatgctca atcagcgcct gctgaatgaa agccggcgtg ggatgcttgg gctcgccgat
     5161 gtgcattgcg atgggatcgc gtcccgggca gggctccacc ccttgcagca aggctctcag
     5221 cttctgaaac ggataggttt gcagcaactc gaggtcggga ttcacaatga ggagacgtac
     5281 acggtcagcg gttcgcaggc aaagtcccga attataaggg cttatgtcgc ccggcccgta
     5341 ggctcatgag gccgggtcat ccactgcgcg cactggatat ttctggaagg ccggcaagat
     5401 ccacgaccgt gtggcgttcc caaccgggct tgacgtgctc ggcaacgacg gtggtgaaaa
     5461 tgcctccccg gacgttgaat cgggcggtga gccgcatata gcgcggtcgc gtggcccgca
     5521 ccaggtcgtc gagaatgcgg ttggtcacgg cttcgtggaa tgcgccctcg tcgcggtacg
     5581 accagacgta gagcttgagg ctcttcagct ccacgcagcg ccggtccggc acgtagtcga
     5641 gtagcagggt cgcgaagtcg ggctgcccgg tgcgcgggca caggcaggta aactccggta
     5701 tttccatgtg aatccggtag tcgcgcgcgg gatgggggtt ggcgaaggtc gcgagcctcc
     5761 ggctcggtgt tttcggcatt atcgcggcgg cccaaatccg ttaaaatatc gttcccaaat
     5821 agcctgcgga ttataacctc acgccagaac gctaaattgc gactcaaaca aatcaatctc
     5881 gcaggcttca agtctttcgt agaccctacc cacatccccg tccccggaca actgtcaggc
     5941 atcgtcggcc ccaacggctg cggcaagtcc aacgtcatcg acgccgtgcg ctgggtgctc
     6001 ggcgaaactt ccgcaaagca cctgcgcggg gacaccatgc aggatatcct gttcaacggc
     6061 tccggccagc gccagccggt gaaccgcgcg agcgtcgagc tgatcttcga caacagcctc
     6121 ggccgggccg ggggacagtg ggcgagctat gccgaaatct cggtgaagcg cgtgctgcag
     6181 cgcgacggcg aagccacctc gtactacatc aacaacgtcc acgtgcggcg gcgcgacgtc
     6241 gccgatatct tcctcggcac cgggctcggc agtcgcgcct acgccatcat cgagcagggc
     6301 atgatctccc gggtgatcga ggccaagccg gaggagctgc gcatcttcct cgaggaggcg
     6361 gccggcgtat ccaagtatcg cgagcgccgg cgcgagaccg aataccgcct gaaggatacg
     6421 cgtgagaacc tgctgcgcgt ggacgacgtg cgcatggagc tcgacaagca gctcgagcac
     6481 ctgcagtccc aggcggaggt cgcggcgcgc tatcacgagc tgcaggcccg gctgcggacg
     6541 gtacagaacc tgttgtggct gctgaggaag caggaggcgg gcgcgcagcg ggcgcgtcac
     6601 ctcaaggaaa tcgagcggct taccctggag ctggaggccg aaaccgcgcg gttgcgcgag
     6661 accgagaagc ggctcgagga catgcgagcg caacactatc gtggcagcga cgaggcgcat
     6721 gccgcccagg gcgccttcta cgaggccaat gccgagaccg cacgcctcga gcaggaactc
     6781 agccacttgc gcgagaaccg ccagcgcgtg gaacgggagc tctcggatct gcacgcccag
     6841 gtcggacgcg gccgcgagca gctcgctgcg gcacaagcga gtcttgccga attgagaaac
     6901 gagcgtgacc gcgcggcgga aagcgtcatg gcgcgcgaag ctgcggtcgg gagcgaaggc
     6961 gacaagctgc cggtggcgga agaggcgttc agggcgaccc gcaaccgtta cgacgagctg
     7021 cagcgcgcgc tcgcgctgag cgagcaggcc atgggcgtgg agcagaccaa gcgcgcacat
     7081 gccgaacaaa tgctggagca gctggcgctg cgcgcgcaac gtctgcgcga agaggatacg
     7141 gcgctgcaac cagcggacgc cccgcggctc cagcggctgc gcggggatat tgcggcactc
     7201 gacgaagagc tgcatcgcct gcacgctgcc atggagtccg aggaggagcg gctgcccggg
     7261 gcggagcagg cgatgcgcga aaggggcgtc gcgcaggagc ttgccggcca gcgtctcgcc
     7321 ggtctggaag cgcagcggca ggccttgacc cggttgcagg aacgccttgc gcggggcgcc
     7381 aatacgaagg actggctcga gacgcatggc ctcggcaagg cgccgcggct ttggcagcaa
     7441 atccgcatcg aatccggctg ggaggacgcg ctggaggcgg tgcttcatca gcgcctgaat
     7501 gccatcgcgc tcgagcgcat ggaacagtct ggacgctggt tcaacgagcc gccaccgggg
     7561 aggatcaccg tgttcgcgcc cgcacgcgag gcggc
//
LOCUS       DBNGWZ010000449         2630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3331373, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000449 DBNGWZ010000000
VERSION     DBNGWZ010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3331373"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC16CX_13750"
     CDS             <1..780
                     /locus_tag="AC16CX_13750"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR3013610.1"
                     /translation="VRIVHETPVSALLDGGNNVGYIAAYRAAEVAIRKARDAGIASVG
                     TYNSYFSGRNAYFVEKIVRERLVAFHIASAYPRVLPPGAARPMLGTNPICFGFPSANG
                     PVIVDMGTASIMWGEVMLHAHLGKPLPEGIGFDEHGNPTRDAEAVLKGGVAPFGGHKG
                     YALSFAIQALGLLAGAALTRGQVRDYGFLFWVLDPEVMLPGGQFMSQMSEYVRQIREA
                     PRRHGVAEIRVPSERAFRERERRRKEGIVIEHKVVEALRAL"
     gene            803..1771
                     /locus_tag="AC16CX_13755"
     CDS             803..1771
                     /locus_tag="AC16CX_13755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013611.1"
                     /translation="MINRPLLSLASLLAALLCGSALAQSYPSKPIRLILPFPPGSPSD
                     MVGRTIGQKIASQMGQNIVPDNRPGAGGTLGLTVAAKSPPDGYNILVTSPTIAISPLL
                     YANLPFDSLRDFTPVARLATIENVMVVHPSVPAKTLKEFIALARRYPGKLNYGSGGAG
                     TTNHLANALLQSLQKIKMTHVPYKGATLAATALMSGEVDEVIIGVAPALPFIKAGRVR
                     PLAVLSEKRVKTLPDVPTGEEAGVPGFKISIWYGMFAPAGTPQEAVRRLHAEVTKALQ
                     DPELLQRMSNAGMDPWLGTPEDLDKHLRSEIQRFSRLAKTAGLKPQ"
     gene            complement(2098..>2630)
                     /locus_tag="AC16CX_13760"
     CDS             complement(2098..>2630)
                     /locus_tag="AC16CX_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HIR3013612.1"
                     /translation="NMQVAITIAILATFMAICKVKDDNIVQAMQQAQANKLDHWAFYQ
                     ARNIREEVAKSTIAHLQLAAAGSAPAQQAAYQEAIQKYEKFASEQRAKKDELMKQAEQ
                     DQKDYDAANFIDDQFDLSDALLAIAISLLAVTALTHQRWLYYVALVPTGFGVLMGLSG
                     LLGWGIHPDALIRLLS"
BASE COUNT          463 a          842 c          852 g          473 t
ORIGIN      
        1 gtgcgcatcg tgcacgagac gccggtgtcc gcgctgctcg acggcggcaa caacgtcggc
       61 tacatcgccg cctaccgtgc cgcggaagtc gccatccgca aggcgcgaga cgccggcatc
      121 gcgtcggtcg ggacctacaa cagctacttc agcggccgca acgcctactt cgtcgagaag
      181 atcgtgcgcg agcgactggt cgcgttccac attgccagcg cctacccgcg tgtcctcccg
      241 ccgggcgccg cgcgccccat gctcggcacg aatcccatct gcttcggctt tccctctgcg
      301 aacggtcccg tgatcgtgga catgggcacc gcctcgatca tgtggggcga agtcatgctg
      361 cacgcgcacc tcgggaagcc gctgcccgaa ggcatcggct ttgacgagca cgggaatccc
      421 acgcgcgatg ccgaagcggt gctgaagggc ggagtggcgc cgttcggcgg ccataagggc
      481 tacgccctgt cgttcgccat ccaggcgctg ggcctgctgg cgggtgccgc cctgacccgc
      541 ggccaggtgc gcgactacgg gttcctcttc tgggtcctcg acccggaagt catgcttcct
      601 ggcgggcagt tcatgagcca gatgtcggaa tacgtgcgcc agattcggga ggcgcctcgc
      661 cggcacggcg tcgccgagat ccgcgtgccg tccgagcgcg cctttcgcga gcgcgagcgg
      721 cgccggaagg agggcatcgt catcgagcac aaggtcgtcg aagcgcttcg cgccctgtag
      781 agttcatctc tgggaggaga agatgatcaa tcgaccactg ctgagtctcg cctcgctgct
      841 cgcagcgctg ctgtgtggca gtgcgctcgc gcaatcctat ccgagcaagc ccatccggct
      901 gatcctgccg tttccgcccg gctcgccgag cgacatggtg gggcgcacga tcgggcagaa
      961 aatcgcctcg cagatggggc agaacatcgt gccggacaac cgccccgggg cgggcggcac
     1021 gctggggctg accgtcgcgg cgaagtcccc gccggatggc tacaacatcc tcgtcacttc
     1081 gccgacgatc gccattagcc cgttgctcta cgcgaacctg ccgttcgact cactacgcga
     1141 ctttaccccg gtcgcgcgcc tcgccaccat cgagaatgtc atggtggtac atccctcggt
     1201 gcctgcgaag accttgaagg agttcatcgc actcgcccgc aggtatcccg gcaagctcaa
     1261 ttacggctcc ggcggcgcag gcacgacgaa ccacctcgcg aacgcgctgc tgcagagcct
     1321 gcagaagatc aagatgaccc acgtgccgta caagggcgcg acgctggccg ctaccgcgct
     1381 catgagcggg gaagtggacg aagtgatcat cggcgttgcg ccagcgctgc cgttcatcaa
     1441 ggcgggacgc gtgcgaccgc tcgcggtttt gtcggagaag cgcgtgaaga cgcttcccga
     1501 cgtgccgacc ggcgaggaag ccggggtacc cggcttcaag atctcgatct ggtacgggat
     1561 gttcgcgccc gccggcacgc cgcaggaggc ggtgaggcgc ctgcacgcgg aggtgacgaa
     1621 agcgctgcag gacccggagc tgctccagcg catgagcaac gctggcatgg atccctggct
     1681 cggcacgccc gaagacctcg acaagcacct gcgcagcgag atccaacgct tctcgcgcct
     1741 cgcgaagacg gcgggcctca agccgcaatg actttggcct taccttggcc ttaccgtgtg
     1801 aataaatcgg gttgaataaa tcgggtcagt gtaacttttt cattgtcttc gcggccttgg
     1861 gcttgggttc tagttatcgt cctcttagcg cctttgcggc tttggcgatc cgcagtacgc
     1921 ctttccttca cggacttggc gtgcttagcg acccgtcgta atcaaaggca gaaggcagaa
     1981 ggatgaaggc agaagagccc agagcggcct aaggcagaag gcagaagggg agcggaagag
     2041 cagggctgag ggaactcgtc actgcatgac cgccaattca atgacgcgat ggaacgccta
     2101 cgacaggagg cgtatcaggg cgtcggggtg tatgccccaa cccaacaggc cggacaagcc
     2161 catgagaacg ccgaacccgg tggggaccag cgcgacgtaa tagagccagc gctggtgggt
     2221 cagcgccgtg accgcgagca gggaaatggc gatcgcgagc agggcgtcgg acaggtcgaa
     2281 ctggtcgtcg atgaagttcg ccgcgtcgta gtctttctgg tcctgctcgg cctgcttcat
     2341 cagctcgtct tttttcgcgc gctgctcgga cgcgaatttt tcgtacttct gtatcgcttc
     2401 ctgataggcc gcctgctgcg ccggcgcgct tccggcggcc gccagttgca gatgcgcgat
     2461 ggtggacttg gcgacttcct cgcggatatt gcgcgcctgg tagaacgccc aatgatcgag
     2521 cttgttcgcc tgcgcctgct gcatggcctg cacgatgttg tcgtccttca ccttgcagat
     2581 ggccatgaag gtggcgagta tcgcgatcgt gatcgccacc tgcatgttca
//
LOCUS       DBNGWZ010000450        17491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3207100, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000450 DBNGWZ010000000
VERSION     DBNGWZ010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17491
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3207100"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16CX_13765"
     CDS             <1..287
                     /locus_tag="AC16CX_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3013613.1"
                     /translation="AAGDTAYDRVWRLPLWDDYQEQLKSNFADFANIGGRPAGVVTAA
                     CFLARFTKKYKWAHLDIAGTAWKTGKEKGATGRPVPLLTQFLIGRAQRGR"
     gene            378..812
                     /locus_tag="AC16CX_13770"
     CDS             378..812
                     /locus_tag="AC16CX_13770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR3013614.1"
                     /translation="MTQIDFYTHVEDKLRTACQLSAKAFAHGLRITIFCPDPETAHRF
                     DRMLWTTSSIGFVPHCAPDDPLAQETPVIVDCQGEKLLHDEVLINLRPEWPPFFSRFQ
                     RLIEIVSLDDGDRASARERFRFYRDRGYEIRTHDLSKPGNAQ"
     gene            1365..4316
                     /locus_tag="AC16CX_13775"
     CDS             1365..4316
                     /locus_tag="AC16CX_13775"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR3013615.1"
                     /translation="MPAFSDPATALAKSFEPAAIEAHWYPIWENNSHFAAGLDETNPN
                     NYCILLPPPNVTGTLHMGHAFQQTLMDALTRYHRMRGFNTLWQPGTDHAGIATQIVVE
                     RQLEAEGASRQALGRDRFVERVWKWKEESGSAITRQMRRLGASCDWGRERFTMDEGLS
                     RVVTEVFVRLYENGLVYRGKRLVNWDPVLQTAVSDLEVESNEEEGKLYHIRYPLADGT
                     GHVTVATTRPETLLGDVAVAVHPEDERYRGLIGKKVRLPLTERQIPVIADAYVDREFG
                     TGCLKITPGHDFNDFNVWLRHREVIAQSLKNAGYQQAYPPSIFKKDARLKDSPVDDFE
                     FGRRAAAGGPTHADDGSGLPGKSSSELLPAAYRGLDRFEARSRVLADLEAGGLLAEAK
                     PHKMMVPRCGRTDAVVEPMLTDQWFVKMESLAKSALEAVARGDIRFVPENWTTTYNQW
                     LTHIQDWCISRQLWWGHRIPAWYDSEGNVYVGRGKQEAQAKHRQAMLGRVRTGGKVTP
                     SELKQDEDVLDTWFSSALWPFSTLGWPADNPALRLYLPSSVLVTGFDIIFFWVARMVM
                     MTLPFTGKAPFREVYVHGLVRDAEGQKMSKSKGNILDPLDLIDGIGLEALLEKRTSNL
                     LNPKQAEAIAAATRRQFPNGIPAFGTDALRFTFASLASHGRDIKFDLSRCDGHRNFCN
                     KLWNATRFVLMNCDGKDTGLDEKLPVELSVADRWIVSRLQRAETEAHQAFRDYRFDNL
                     ARAIYELTWNEYCDWYVELAKVQLADGDEARQRATRRTLTRVLEAILRLAHPVIPFIT
                     EELWQKVAPLAGKHGPSIMLARYPEPQPEKLDEPAEREMDQLMALTNACRTLRAEMNI
                     APSQKLPLLIQGDRDRLAPFVPYLTALARLSEVVIVEGALPAAEAPVSIVGEFRLMLK
                     VEVDVTAERERLHKERLRLEAEIEKCEAKLANPDFVRRAPPLIVAQEKERLARFKAML
                     DKINEQLAKLA"
     gene            4355..5602
                     /locus_tag="AC16CX_13780"
     CDS             4355..5602
                     /locus_tag="AC16CX_13780"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013616.1"
                     /translation="MIHSLRAFNHRNFRLFFFGLSLGVIGYWVQQIAMSWLVYRLTGS
                     AWLLGVTAFAGQIAVLVLAPFGGLWADRVDRRKLLIVTQAAAAMPAFALAVLAYLDVI
                     EVWHVIVLAAVLGVVMAIDAPIRQSFLPEIVPGTQDLPSAIAFNSGMYNAARMIGPTI
                     AGVLLTATSEAFCFLVNGMTKIAALIPLVMMVIRPHERPSAQVPMRRAFREGVVYAWE
                     LVPVRLLLTVVALVSFMATPYQALMPIFAAEVLAGGADMLGFLMGAAGLGGLLGIILL
                     ASRKELRGLLRWIVAACFCAGAALAVFSYSTQLALSLAMLMVVGASIVIAVNGISTIT
                     LTIVEDRMRGRVSGFYSMAFLGMYPLGALAAGALASWIGAMRTLTIGGACCVLCALWL
                     WPKLPGLRRHLRPIYVRLGIINE"
     gene            complement(5680..6384)
                     /locus_tag="AC16CX_13785"
     CDS             complement(5680..6384)
                     /locus_tag="AC16CX_13785"
                     /inference="COORDINATES: protein motif:HMM:NF022041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3013617.1"
                     /translation="MIRLYQFPPAFGLPNASPFCMKVETYLRMAGLAYECPRGASHFK
                     APKGKLPYIEDNGRVVADSTFIIEYLKQAYGDPLDAHLNAPERATALAYQRLLEENFY
                     WAVIYSRWMDEAGFSRAREAFFAKLPAPLKLVLPHLARRGIRGELRGHGMGRHAREEI
                     YAIGCRDLSALADFLGDKPYFMGRQPTSLDASAYGFLANVLWVPVESPLKAHARQRPN
                     LEAYCRRMKERYYGDE"
     gene            complement(6498..7739)
                     /locus_tag="AC16CX_13790"
     CDS             complement(6498..7739)
                     /locus_tag="AC16CX_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR3013618.1"
                     /translation="MRLRKLVVVLVATGCFVPGLATATNGYFQHGYGIKSKSMAGVGI
                     ALPQDAIAAATNPAGMVWVGDRIDFGLDWFRPVRNTRLSLGPGPFGAEYDGNGDQNFL
                     IPEFGYNKMLNPNSSLGVSVYANGGMNTSYDTLNPATGGAIFGQGRLGVDLSQLFIAP
                     TYSVKLNPNHSLGASLNLAYQRFKAYGIQNFDNAFVSSAPGAVTNNGYDSSVGWGIRI
                     GWLGQVTPAVSVGATYQTKTHMQKFDKYRGLFAEQGGFDIPDNYGVGIAVKTTPKLTL
                     AADVQQINYSKVKSVGNTGNIATQLGTSNGPGFGWRDMTVLKLGASYAMSDRLVLRGG
                     LSHGRQPIPSTETFFNVLAPGVMEDHLTLGLTWTLPNKSELTFGYMHAFKKSVTGTGP
                     TTGFNFNMYEDALGVAYGWRM"
     gene            complement(7784..8401)
                     /gene="dsrE2"
                     /locus_tag="AC16CX_13795"
     CDS             complement(7784..8401)
                     /gene="dsrE2"
                     /locus_tag="AC16CX_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein DsrE2"
                     /protein_id="HIR3013619.1"
                     /translation="MGVNPDTALLDDLIKRRLDEILPQKLEEKLAELREKKTPSMTII
                     ATKGTLDWAYPPFILASTAGALGWEVSVFFTFYGLNLLKKDLSDLKVSPLGNPGMPMK
                     MPFGPDWFKSINWNIPNIVEAGIPGFESLATTLMKQTIKNKGVAGIEELRQACIEADV
                     KLIGCQMTVDLFGYTRDDFIPEVKDFVGAATFLPMARDADVSLFI"
     gene            complement(8491..8724)
                     /locus_tag="AC16CX_13800"
     CDS             complement(8491..8724)
                     /locus_tag="AC16CX_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR3013620.1"
                     /translation="MQASNAVLDAKGLNCPLPILKAKKALQGVPAGGLLEIHATDPGA
                     VQDFQAFCRSTGNELIASSQEGNVFKFVIKRIK"
     gene            8882..10087
                     /locus_tag="AC16CX_13805"
     CDS             8882..10087
                     /locus_tag="AC16CX_13805"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HIR3013621.1"
                     /translation="MTDSRMALPARSNRLAPVFVGHEIYRQAAYGNHHPLAIPRVAPV
                     VDLCRALGWLVDGEFVVSPRASDEQLAWFHAPEYVSALRHAAEAGAVATPARQRFAIG
                     TMENPLFPGVFERAATSVGGSMRAAELALEGRLTYHPAGGTHHGRPDRASGFCYFNDP
                     VFAILVLLRAGMERVLYVDLDAHHGDGVQDAFERDPRVHTISIHEQGRWPHTGGLSDT
                     GAGRACNLPVPPRFNDRELDVLVGDVVLPLAHRVSPQVVVITCGADALDGDPLSSMAL
                     SNVALWSVVERLAALAPAVVVLGGGGYNPWTVARYWTGLWGRLSGRDLPATLPRQAHE
                     ILCRLRCDLIDEEDMRAEWLTTLADKPHQGAVRDEIGALAEQALASVNEAFWQPGMAY
                     TAPRRFPVS"
     gene            10094..11137
                     /locus_tag="AC16CX_13810"
     CDS             10094..11137
                     /locus_tag="AC16CX_13810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3013622.1"
                     /translation="MITSWIDRLAAIEELEGAGLDAGLIAECECEARALPGREIRFST
                     PTFKTYSSCELSGCGKNSFPAFSVTAAGCALMCDHCQAKILEPMIPAVSPEALDRKVR
                     EMIESQELRGFLLSGGSNRRNEIRYERFYPVVEKLKHDFPHLKIAVHSALLDEPRARA
                     MEAAGVDTAMMDVIGSRKTIAEVYHLDRPVEDFEATLAALCATSMEVVPHIVIGLHYG
                     RILGEPNALDIVSRHRVHSLVLVVVMPFYARAGTFKTPETSEVGRVFLEARRRVSDRQ
                     VLLGCARPPGMHRRVTDAYAVMAGLDGIAFPAEGTLAVATAIGRPAVQEHACCSIRIG
                     NAIRLGNRAAVAV"
     gene            11137..12207
                     /locus_tag="AC16CX_13815"
     CDS             11137..12207
                     /locus_tag="AC16CX_13815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR3013623.1"
                     /translation="MASERIEVLVIGLGPAGASAALEAAGRGCNVIAVDRKREPGKPV
                     QCAEFVPAMIGVEARKLAASVRQPIRAMVTFVEDDAPDVKEDFPGYMLDRTVFDATLV
                     SAAMQAGARCRFGAAVRSITREGCVELADGQRVAASVLIGADGPRSIVGRRIGQRNRQ
                     LVETRQITVPLRLRHEATDIFLSADIPGGYGWLFPKGEVAHLGAGVDPAHKSRLKKIL
                     ADLHAALVDSGRAGAEILALTGGAIPVGGMLKPWGTLGDTLALLAGDAAGLTNPVTGA
                     GIAAAVHSGTLAGQAAAEWVGGDREAGADFEEELRAVFQSALDRAAARRRELAALSRA
                     GALPDKAALRRGWIAYPEYWAA"
     gene            12254..13483
                     /locus_tag="AC16CX_13820"
     CDS             12254..13483
                     /locus_tag="AC16CX_13820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3013624.1"
                     /translation="MASTATAERDYNPPELMQPRAPAATPREMKNGARVKANNVAPQG
                     VYLPNYNILTPHMRSPEYVQMSTAAAITLGIMRGRMYRCSCTRCLNLLLTYPEGCRAN
                     CAYCGLARHREAERDYADRNFIRVDWPAVPLDTMIDIVARDGTASPFHRMCISMITHP
                     RSDADTVTVLRKWTDRVDPSAIPVSILSNPTTMTRDDVKRLRELGADIFTVALDAATP
                     EIFDRTRGKGVQSPHSWAKYWEILMDARNIFGPGKFGAHIIVGMGETEHDVLSLVQRL
                     VDLGGHSHMFCFFPEKGSLMDHLPATPRDQWRRVQLGRYLIDYRGARVERMLFDGQGR
                     VTGYGVAASEVEAVIEEGIAFRTSGCPGKFRDDVSACDRPYGDSPPSDIASYPFQPGK
                     GDRRRIRRQLRLVETRD"
     gene            13488..14552
                     /locus_tag="AC16CX_13825"
     CDS             13488..14552
                     /locus_tag="AC16CX_13825"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR3013625.1"
                     /translation="MAAVFRVIDTGIRDGRRQIAFDQAMIDAHKADAIPDSIRFLRFP
                     PTALIGRHQVLAREINLDYCRANGVGIVRRITGGGAIYFDPGQLGWELVFHRSTLGIG
                     SLAELTRGICEAAAAGLSRLGVSANYRPRNDIEVDGRKVSGTGGFFDEDTLFYQGTVL
                     VDMNPADMIAALNVPQAKLAKRSLDSAAQRVVTLKELLGAAPAIESVKLSLLAGFAEQ
                     LGITPVPGEITSHEEALAAKNHDEEIGTEAFVAGIDDPGADEGVLSASRTGAGGTIQA
                     YVRLEGARRDRIREALITGDFFVTPPRTILDLEASLRNVAVAEAGATIERFFADTKVG
                     LLTAAPADFRKVVEDAIASA"
     gene            14710..15420
                     /locus_tag="AC16CX_13830"
     CDS             14710..15420
                     /locus_tag="AC16CX_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013626.1"
                     /translation="MRLISVVQHTSAEYLGLIEDHLEGRGIRFKYFRPFTEGGGLPDR
                     DAIRDGLVLLGGGVWGAAGTRDVPTLKQETGLAHAALMYGSPVIGIGLGAQILAIAAG
                     GRSADGPLTFEVTTARRLDASALNGYLPEQYPLAIYMRDWPVPPAYARVLAEDPKGRP
                     ALWQIGDCAFGFAGHPGLKVAMVEDLIMEFTEAPADPGPELAKLRSAQRALEDALVPI
                     MTGLVQLTKWMQPRGPAV"
     gene            15596..15973
                     /locus_tag="AC16CX_13835"
     CDS             15596..15973
                     /locus_tag="AC16CX_13835"
                     /inference="COORDINATES: protein motif:HMM:NF014672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3013627.1"
                     /translation="MAGKLIIVMANTDPRNGEELGAPIFQATVAAAMEYEVEVICTAT
                     AGRLMKKGVAEKLHVKEGSPKSVYDFIKDAHEAGVKFYCCSPNLDLFDMKESDLIPEC
                     SGIIGGAHLIEEVMEGDCKVMTY"
     gene            15983..16738
                     /locus_tag="AC16CX_13840"
     CDS             15983..16738
                     /locus_tag="AC16CX_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3013628.1"
                     /translation="MAHATATRVQEHIGDPVSEKPAVARAKLEEIFKDIQSDLRYDHE
                     LNGCLNCGICTATCPAAHYYDFSPREIVQLLWTENLEGIYDAMQEKIWACAQCYTCAA
                     RCPFGNSPGGLVMLMREAAIKHGMESAKNVLRPFSRVLLKLISTGNQLSPDMINRDHF
                     ADWGPNVSKIDAPLKVLRKAIPMPTLNTTVTAWEVNLKTSVELYTIWEETGVLKQLET
                     VDENLFDVIQDIMDEKREDWKDFLAEQEEAKDD"
     gene            16748..>17491
                     /locus_tag="AC16CX_13845"
     CDS             16748..>17491
                     /locus_tag="AC16CX_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013629.1"
                     /translation="MDKQTPGNERFTGHGSEWRDANLSPTEARMATAWVEQKIDRRSM
                     LTNKERVQDVRDIMWQLEKDGEIVVHRVSDEHEPVMVKTLYGWDKKIPTRRLWHHKSC
                     GQCGNIPGYPASLLWLMNRLGIEYLDETDQTSCTAWNYHGSGIGNLESLAAVFLRNFH
                     QAYVAAKAQGLPEAYFYPLVHCGTSFGNYKEVRSYLLLSAKLRERVRQSLGKLGRLVD
                     GKLLIPEEVVHYSEWVHVMRHRIASLQEID"
BASE COUNT         3075 a         5754 c         5661 g         3001 t
ORIGIN      
        1 cagccgcggg cgacaccgca tacgacaggg tatggcgcct gccgctgtgg gacgattacc
       61 aggaacagtt gaagagcaac ttcgccgact tcgccaacat cggcgggcgc cccgccggcg
      121 tggtgacggc ggcctgcttt ctagcccgct tcacgaagaa atacaaatgg gcccacctcg
      181 acatcgccgg cactgcctgg aagaccggca aggaaaaggg cgcgactggc cgaccggtgc
      241 cgctattgac ccagtttctc atcggccggg cgcagcgcgg ccgatgagga gtgctgagtg
      301 caaagtgctg agtgcaaagt ttacggcttt gccgctttcc gctcgtcact catcacacat
      361 cactcatcac tgaaggtatg acgcaaatcg acttctatac ccacgtcgag gacaagctgc
      421 gcaccgcctg ccagctttcg gcgaaggcat tcgcgcacgg gctcaggatc acgatcttct
      481 gccccgatcc cgaaaccgcc catcgcttcg accgcatgct gtggacgaca tcctcgatcg
      541 gattcgtgcc gcattgcgcg ccggacgatc ccctggcgca ggagacgccg gtcatcgtgg
      601 attgccaggg cgaaaaactg ctgcacgacg aggtgctgat caacctgcgt ccggagtggc
      661 cgccgttctt cagccgcttc cagcgcctga tcgagatcgt cagcctcgac gatggtgacc
      721 gcgccagcgc ccgcgagcgt ttccgcttct atcgtgatcg cggctacgag atccgcacgc
      781 atgatttgag caagccggga aacgcccagt agccggccat gccagaatcc cggttcccac
      841 gcgacgacgg cgacgatctt ctcaagcgcg ccgatgcgct gctcgcgcgc caccgcggcg
      901 cggcgaagtc cgatgccgcg gccggcgcgg acctcccggt gctgacggaa ccggcggcgg
      961 gtcctgccgg ggataccgag attcccacgc tcacggagat cgttcccgca ggcgagctgc
     1021 ccgccctcct cgccgcaccc ggcaccggct cgagggaaac atccgccgcc ggcgaggtga
     1081 tctcgcgcgt gcaggtgcag aacctcgagc acagtgtcta ccagaagctg aagcgcgatc
     1141 tcgatcgcca catcgaacag gtcatgcagg accgcttctt gccggacatc ggggcggcgc
     1201 tcgacgcggc gctcgccaag gtgggccagg acatccgttc cgacatcaac cagatggtgc
     1261 gcgcctcgat cgaggagacg ttgcgcaccc agatcaagaa tctgcgcgtc gcggtggagg
     1321 ccaaggccgc gatgcgggaa gccgccgcgg ccgctcccgg tatcatgccc gctttttccg
     1381 atccagcgac ggcgctcgcc aagagtttcg agccggcggc gatcgaggcc cattggtatc
     1441 cgatctggga gaacaacagc cacttcgcgg ccggcctcga cgaaaccaat cccaacaact
     1501 actgcatcct gctgccgccg cccaacgtga cgggcacgct gcacatgggc cacgcgttcc
     1561 agcagacgct gatggacgcc ctcacccgct accaccgcat gcgcgggttc aacacgctgt
     1621 ggcagccggg cacggaccac gccggcatcg cgacgcagat cgtggtcgag cgccagctcg
     1681 aggccgaagg cgcctcgcgc caggccctcg gccgcgaccg attcgtcgag cgggtctgga
     1741 agtggaagga ggagtcgggc tcggccatca cgcgccagat gcgccggttg ggcgcctcct
     1801 gcgactgggg gcgcgagcgc ttcaccatgg acgaggggct ctcgcgcgtg gtgacggagg
     1861 tgttcgtgcg gctctacgaa aacgggcttg tctaccgggg aaaacgcctg gtcaactggg
     1921 accctgtgct gcagacggcg gtgtccgacc tggaggtcga gtcgaacgag gaggaaggca
     1981 agctctacca catccgttac ccgctcgccg acggcacggg gcatgtcacg gtcgcgacca
     2041 cgcggccgga gacgttgctc ggcgacgtcg ctgtcgcggt gcatcccgag gacgagcgct
     2101 accggggctt gattggcaag aaagtgcgcc tgcccctcac cgagcggcag attcccgtga
     2161 tcgcggacgc ttacgtcgac cgcgagttcg gcaccggttg cctgaagatc acgccggggc
     2221 acgatttcaa tgacttcaac gtctggctgc gccataggga agtcattgcg cagtccctca
     2281 agaacgccgg ctatcaacaa gcctaccccc ccagcatctt caaaaaagac gcccgactga
     2341 aggattctcc cgtcgacgac tttgaattcg gccgcagggc ggcggcagga ggcccgactc
     2401 acgcggacga cgggtcgggt cttccgggca agtccagttc cgaactgctg cccgccgcct
     2461 accgggggct cgaccgtttc gaggcgcgca gccgcgtgct ggccgacctc gaggccgggg
     2521 gactgctcgc cgaggcgaag ccgcacaaga tgatggtgcc gcgctgcggg cgcaccgacg
     2581 ccgtggtcga gccgatgctg acggaccagt ggttcgtgaa gatggagtca ttggcgaagt
     2641 ccgcgctgga ggcggtggcg cgcggcgaca tccggttcgt gccggaaaac tggaccacca
     2701 cctacaacca gtggctcacc cacatccagg actggtgcat ctcgcggcaa ctctggtggg
     2761 gccaccgcat tccggcgtgg tacgactcgg aaggcaacgt ctatgtcggc cgcggcaaac
     2821 aggaggccca ggcgaagcac cggcaggcga tgctcgggcg ggtgcggacg gggggcaaag
     2881 tgacgccctc ggagctcaag caggacgaag acgtgctcga cacctggttc tcctccgccc
     2941 tgtggccgtt ctcgacgctc ggctggcccg cggacaaccc ggcgctgcgt ctgtatctgc
     3001 cctcgagcgt gctggtcacg ggattcgaca tcatcttctt ctgggtggcg cgcatggtga
     3061 tgatgacgct gcccttcacc gggaaggcgc cgttccgtga agtctacgtg cacggcctgg
     3121 tgcgggatgc cgaggggcag aagatgtcga agtcgaaggg caacattctc gatcccctcg
     3181 atctcatcga cggtatcggg ctcgaagcgc tcctcgagaa gcgcacttcc aacctcctta
     3241 acccgaagca ggccgaggcg attgccgcgg ccacccggcg ccagtttccg aacggcatcc
     3301 ccgccttcgg caccgatgcg ctgcgcttca ccttcgcgag cctcgcctcg cacggccgcg
     3361 acatcaaatt cgatctgtcc cgctgcgacg gtcaccgcaa cttctgcaac aagctgtgga
     3421 acgcgacgcg cttcgtgctg atgaattgcg atggcaagga caccggcctc gacgagaagc
     3481 tgccggtcga gctctcggtg gccgatcgct ggatcgtgag ccgactgcag cgggccgaga
     3541 cggaggcaca tcaggcattc cgcgattatc gcttcgacaa ccttgcccgc gccatctacg
     3601 agctcacgtg gaacgaatat tgcgactggt acgtcgagct ggcgaaagtg cagctcgccg
     3661 acggcgatga agcccgccag cgcgctaccc gccgcacgct cacgcgcgtg ctggaggcga
     3721 tcctgcggct cgcgcatccg gtgatcccgt tcatcaccga ggagctgtgg cagaaggtgg
     3781 cgccgcttgc gggcaaacac gggccttcca tcatgctcgc ccgctacccc gagccgcagc
     3841 cggagaagct ggacgagccg gcggaacggg aaatggatca gctcatggcg ctcaccaacg
     3901 cctgccggac gctccgcgcc gagatgaaca tcgccccctc ccagaagctg ccgctgctga
     3961 tccagggcga tcgggaccgg ctcgcgccgt tcgtgcccta tctcaccgcg ctggcgcggc
     4021 tgtcggaggt cgtgatcgtc gagggcgcgc tgccggcggc ggaggcgccg gtgtccatcg
     4081 tcggcgagtt ccgcctgatg ctcaaggtcg aggtggacgt caccgccgag cgcgagcggc
     4141 tgcacaagga gcggctgcgg ctggaagccg agattgagaa atgcgaggcc aagctcgcca
     4201 atcctgattt cgtaaggcgc gccccgcccc tgatcgtggc ccaggaaaag gaacgacttg
     4261 cccgcttcaa ggcgatgctc gacaagatca acgaacagct cgccaaactc gcctgacggc
     4321 gggctcccgt cccgcaggca cctgggccgg cgcgatgatt cactccctgc gcgcgttcaa
     4381 tcaccgcaac ttccggctgt ttttcttcgg cctgtcgctc ggcgtcatcg gctactgggt
     4441 gcagcagatc gccatgagct ggctggtgta ccgcctgacg ggctcggcct ggctgctggg
     4501 cgtgaccgcc ttcgccggac agatcgcggt gctcgtgctc gcgccgttcg gcggactgtg
     4561 ggccgaccgc gtggatcgca ggaagcttct gatcgtgacc caggcagccg cggcgatgcc
     4621 ggctttcgcg ctcgccgtgc tcgcctacct ggacgtgatc gaagtgtggc acgtcatcgt
     4681 gctggcagcg gttctcggcg tggtgatggc gatcgacgcg cccatccgcc agtcctttct
     4741 gcccgagatc gtgcccggca cgcaggatct gccgagcgca atcgccttca attccggcat
     4801 gtacaacgcc gcacgcatga tcggcccgac cattgccggc gtgctgctca cggccacaag
     4861 cgaagcgttc tgtttcctgg tgaatggcat gaccaagatc gccgcgctca ttcccctcgt
     4921 catgatggtg atcaggcccc acgagcggcc ttccgcgcag gtaccgatgc ggcgggcatt
     4981 cagggagggc gtggtttacg cctgggagct ggtgccggtc cggctgctgc tgacggtcgt
     5041 cgcgctggtg agcttcatgg cgaccccgta ccaggcgttg atgccgattt tcgcggcgga
     5101 agtgctggcc ggcggcgcgg acatgctcgg gttcctgatg ggcgcggcgg gactgggcgg
     5161 ccttctcggc atcatcttgc tcgcctcgcg caaggagctg cgcgggttgc tgcgctggat
     5221 cgtcgcggcc tgcttctgcg ccggcgcagc gctcgccgtt ttttcctatt cgacccagct
     5281 cgcgctctcg ctcgcgatgc tgatggtcgt cggggcaagc atcgtgatcg ccgtcaacgg
     5341 catatccacg atcacgttga ccatcgtcga ggaccgcatg cgcgggcgcg tgagcggctt
     5401 ctacagcatg gctttcctcg gcatgtatcc cctgggcgcc ctcgccgcgg gcgcgctggc
     5461 gtcctggatc ggtgccatgc gcacgctgac gatcggcggc gcgtgctgtg tgttgtgcgc
     5521 gctgtggctg tggccgaagc tccccgggct acggcgccac ctccggccga tctacgtgag
     5581 gctggggatc atcaacgagt gaggcgtgaa aggtgataag tgataggtga taggaacaaa
     5641 cctccacgcg agcgcgggta acttatcacc tgtcacccgt cactcatcac cgtagtatcg
     5701 ctccttcatc ctgcgacagt acgcctcgag attcggtcgc tgccgcgcat gtgccttgag
     5761 cggggattcc accggcaccc acagcacgtt ggcgagaaag ccgtaagcgc tggcgtcgag
     5821 tgaagtcggc tgccgcccca tgaaatacgg cttgtcgccg aggaaatcgg cgagtgcgga
     5881 taggtcgcga cagccgatcg cgtagatttc ctcacgcgcg tgacggccca tgccatggcc
     5941 gcggagctcc ccgcggatgc cgcgccgtgc gagatgcggc agcacgagct tgagcggcgc
     6001 cggcagtttc gcaaaaaagg cttcccgcgc cctcgaaaag cccgcttcgt ccatccagcg
     6061 cgaatagatt accgcccagt agaaattttc ttccagcagc cgctggtagg cgagcgccgt
     6121 cgcgcgttcc ggcgcgttga gatgcgcgtc aagcgggtcg ccgtacgcct gcttgaggta
     6181 ttcgatgatg aaggttgagt ccgcgacgac gcgcccgttg tcctcgatgt agggcagctt
     6241 gcccttcggc gccttgaagt ggctcgcgcc gcgtgggcat tcataggcca gtcccgccat
     6301 gcgcagatac gtttccacct tcatgcagaa ggggctcgcg ttgggcaggc caaacgccgg
     6361 cgggaattgg tagaggcgga tcacacgctc agtatagcga gcgagccggc ccgcgggccc
     6421 agccgcctgg gttgcgtcaa aaaaagaggc cgggtgaccg gcctccgctg atcgatgacg
     6481 tcccgaggga gcgagcgcta catcctccag ccataggcaa cgcctagggc gtcctcatac
     6541 atgttaaagt tgaagccggt agtcgggccg gtgccggtca cgctcttctt gaaagcgtgc
     6601 atgtaaccga aggtcagttc cgacttgttc ggcagcgtcc acgtcagccc caatgtcaga
     6661 tggtcctcca tcactcccgg cgcgaggacg ttgaagaagg tctccgtgct cgggatcggt
     6721 tgccgcccgt ggctcaaacc gccgcgcaac acgagccggt cgctcatggc atagctcgct
     6781 ccgagtttaa gcactgtcat atcgcgccat ccaaacccgg gaccgttgct ggtcccgagc
     6841 tgcgtcgcga tgttgccggt gttgccgacc gacttcacct tgctgtaatt gatttgctgc
     6901 acatcagcgg ccagcgtcag cttcggcgtt gtcttcactg caatgccgac gccgtaattg
     6961 tccggaatgt cgaaaccgcc ttgctcggcg aacagtccgc ggtacttgtc gaatttctgc
     7021 atatgcgtct tggtctgata ggtcgcgccg acgctcactg cgggcgtaac ctgaccgagc
     7081 cagccgatac gaatgcccca gcctaccgaa gaatcgtagc cgttgttggt aaccgcgccc
     7141 ggggccgaag agacgaaggc gttgtcaaag ttctgaatgc cataggcctt gaagcgctgg
     7201 taggcgaggt tgagcgacgc gccgagggaa tggttagggt tcaacttgac cgaataggtc
     7261 ggcgcaataa acagctgcga gaggtcgacc ccgagcctgc cttgaccgaa tatcgcgccg
     7321 cccgtcgcgg gattcaatgt atcgtagctc gtattcatgc cgccgttggc gtagacggag
     7381 acaccgagtg acgagttcgg gttcagcatc ttgttgtagc cgaactcggg tatcaggaaa
     7441 ttctgatcgc cgttgccgtc gtactccgcg ccaaacggcc cggggcccag actcaaccgg
     7501 gtattccgta ccggacgaaa ccagtcaagg ccgaagtcaa tgcggtctcc cacccaaacc
     7561 atgccggcag ggttggtcgc cgccgctatt gcatcttgag gcagagcaat tcccactccc
     7621 gccatgcttt tcgatttgat gccgtagccg tgctggaagt agccgttggt tgccgtggcc
     7681 agtcccggaa cgaagcagcc ggtggcgaca agaacaacaa cgagtttgcg caatctcatg
     7741 atgtctccct tttttgattg ccggccgctc agccgggatt cggtcaaatg aacaaactca
     7801 catcggcgtc ccttgccatc ggcaggaacg tcgccgcgcc gacaaagtcc ttcacttccg
     7861 gaatgaaatc gtcgcgcgtg tatccgaaca ggtcgaccgt catctggcag ccgatcagct
     7921 tgacgtcggc ctcgatacag gcctggcgca gctcttcgat gccggcgaca cccttgttct
     7981 tgatcgtctg cttcatgagg gtggtggcga gcgactcgaa gccgggtatg cccgcttcga
     8041 cgatgttcgg aatgttccaa ttgatggact tgaaccagtc gggaccgaaa ggcattttca
     8101 tcggcatgcc gggattgccg agcgggctta ccttcaggtc gctcaagtcc ttcttgagca
     8161 ggttcaggcc gtagaacgtg aagaacaccg acacctccca tcccagcgcc ccggcggtgg
     8221 aggcgaggat aaagggcgga taggcccagt cgagcgtgcc cttggtcgcg atgatggtca
     8281 tcgagggcgt cttcttctcg cgcagctccg cgagcttctc ctccagcttc tgcggcagga
     8341 tctcgtcgag gcggcgcttg atcagatcat cgagcaatgc ggtatccggg ttcacgccca
     8401 tggccacgct ccttagagac ggtaacgagt ggatactccc ctcgtgctcc cctacgtcag
     8461 cagccgttcc agtcattttg aaacggcctc ttacttgatg cgcttgatga cgaacttgaa
     8521 cacgttgccc tcctggctcg aggcgatcag ctcgtttccg gtcgagcggc agaacgcctg
     8581 gaaatcctgc accgcgcccg gatcggtggc atggatctcg agcagtccgc ccgccggcac
     8641 gccttgcagg gccttcttcg ccttgaggat cggcaacggg caattcaggc ctttcgcatc
     8701 gagcaccgca ttggacgctt gcatccgtcg tctccgagtt ggaagatcat gcggacgctc
     8761 aatgcatttc cccctgcgcc cgccccgcgt gcatattaga tggtctttat attagaaaaa
     8821 ttgaatacta taatgcggtt cttgtcaaga acaatcgcga tgatcacgag gcaggatagc
     8881 cgtgactgac tcaagaatgg cgctcccggc ccgttccaac cggctcgctc cggtattcgt
     8941 cggtcacgag atctatcgcc aggccgcgta cggaaatcac cacccgcttg ccattccccg
     9001 cgtcgccccg gtcgtggatc tctgccgcgc gctcggatgg ctcgtggacg gcgagttcgt
     9061 ggtgagcccg cgtgccagcg atgagcaact cgcgtggttc cacgcgcccg agtacgtgag
     9121 cgcgctgcgc cacgccgccg aagcgggtgc ggtcgccacg ccggccagac agcgcttcgc
     9181 catcggcacg atggagaacc cgcttttccc cggggtcttc gagcgcgcgg cgaccagcgt
     9241 cgggggatcc atgcgcgcgg cggaactcgc gctggaaggc cgcctcacct accacccggc
     9301 gggcggcacg caccacggtc gcccggatcg cgcgagcggt ttctgttatt tcaacgatcc
     9361 ggtgttcgcc attctcgtgt tgctccgggc tggaatggaa cgcgtccttt acgtggatct
     9421 cgacgcgcac cacggcgacg gggtgcagga tgcattcgag cgcgatccgc gcgtgcatac
     9481 catctcgatc cacgagcagg ggcgctggcc gcacaccggc ggtctttccg acacgggcgc
     9541 cggcagggcg tgcaatctcc cggtaccccc gcgcttcaac gaccgcgaac tcgatgtgct
     9601 cgtgggcgac gtcgtgctgc cgctcgccca tcgggtctcg ccccaggttg tcgtgatcac
     9661 ctgcggggcc gatgcgctcg acggcgatcc gttgtcatcc atggcgctgt cgaacgtcgc
     9721 gctctggtcg gtggtcgaac gtctcgcggc gcttgcgccg gcagtcgtcg tgctgggcgg
     9781 aggcggctac aatccatgga cggttgcgcg ctactggacg gggctatggg gacgattgtc
     9841 aggccgcgac cttcccgcaa cgctgccgcg gcaagcgcac gaaatcttgt gccggctccg
     9901 gtgcgatctc atagacgagg aggacatgcg cgcggaatgg ctcactacgc tcgcggacaa
     9961 accacatcag ggtgccgtgc gggatgagat cggcgcgctc gccgagcagg cgctcgcgtc
    10021 cgtgaacgag gcgttctggc aaccggggat ggcctatact gctccccggc gcttcccggt
    10081 aagctgacgg aatatgatca cgagctggat cgatcgactt gccgccatcg aggagctgga
    10141 gggcgccgga ctcgacgccg gcctcatcgc cgagtgcgag tgcgaagcgc gcgccctgcc
    10201 cggccgcgag atccggttct cgacccctac cttcaagacc tactcgagct gcgagctctc
    10261 cggctgcggc aagaacagct ttcccgcctt ctccgtcacc gcggcgggct gcgcgctgat
    10321 gtgcgaccac tgccaggcga agatcctcga gccgatgatc ccggccgtga gccccgaggc
    10381 gctcgatcgc aaggtgcgcg agatgatcga gtcacaagag ctgcggggtt ttcttctctc
    10441 ggggggttcc aaccgtcgca acgagatccg ctacgagcgt ttctacccgg tcgtcgagaa
    10501 actcaagcac gacttcccgc acctcaagat cgcggtccac tcggccctgc tcgacgagcc
    10561 gcgcgcgagg gccatggagg ccgcgggtgt cgataccgcc atgatggacg tcatcggctc
    10621 gcgcaagacg atcgccgagg tctatcacct cgaccgtccg gtcgaggact tcgaggcgac
    10681 gcttgcggcc ctgtgcgcga cctccatgga agtcgtacct catatcgtga tcggactcca
    10741 ttacgggcgc atcctcggcg agccgaacgc gctcgatatc gtgagccgcc accgcgtcca
    10801 ctccctggtg ctggtggtcg tcatgccgtt ctatgcccgc gccggcacct tcaagacgcc
    10861 ggagacgtcg gaagtcgggc gggtctttct cgaggcgcgg agaagggttt ccgaccggca
    10921 ggtgctcctc gggtgcgcgc gcccgcccgg catgcaccgg cgggtgaccg acgcctacgc
    10981 ggtcatggcg ggtctcgacg gcatcgcctt cccggccgag ggcacgctcg cggtcgcaac
    11041 cgcgatcggc cggccggcgg tccaggagca cgcctgctgc tcgatccgga tcgggaacgc
    11101 gatccggctt ggcaatcgcg cggcggtcgc agtgtgatgg caagcgaacg gatcgaggtt
    11161 ctcgtcatcg gcctcggccc ggccggcgcg agcgccgccc tagaggccgc ggggcgcggc
    11221 tgcaacgtca tcgcggtcga tcgcaagcgc gagccgggca aaccggtcca gtgcgcggaa
    11281 ttcgtgccgg cgatgatcgg cgtcgaagcg cggaaactcg ccgcttcggt gcgccagccg
    11341 atccgggcga tggtcacctt cgtcgaggac gacgcgccgg acgtcaagga agatttcccc
    11401 ggctacatgc tggatcgcac ggtgttcgac gcaacgctgg tctctgccgc catgcaggcg
    11461 ggcgcgcgtt gccggttcgg cgcagccgtg cgctcgatca cgcgcgaggg gtgcgtggag
    11521 cttgccgacg gccagcgcgt cgcggcttct gttctcatcg gagcggatgg cccccgctcc
    11581 atcgtcggac gccgaatcgg ccagcgcaac cggcaattgg tggagacgcg gcagatcacg
    11641 gtgccgctgc ggctgcgcca cgaggcaacg gacatcttcc tctcggccga catccccggc
    11701 ggatacggct ggctcttccc caagggtgag gttgcacatc tgggcgcggg tgtcgatccc
    11761 gctcataaat cccggctcaa gaaaatcctc gcagacctgc atgcggccct ggtcgacagc
    11821 gggcgggccg gcgccgaaat cctcgctctc accggcggcg cgattccggt cggcggcatg
    11881 ctgaagccgt ggggaacgct cggcgacacg ctcgcgctgc tcgccggcga tgcggccggc
    11941 ctgaccaatc ccgtgaccgg ggccggcatc gctgctgccg tccattcggg aacactcgcc
    12001 ggccaggctg cggcggagtg ggtcggcggc gatcgcgagg cgggtgcgga ctttgaggaa
    12061 gaattgaggg ccgtatttca gtcggcgctc gaccgggcag cggcacgcag gcgcgagctc
    12121 gccgcgttgt cgcgcgccgg cgcgttgccg gacaaggcgg cgctgcgccg cggctggatc
    12181 gcctatccgg aatattgggc ggcctgacga aggagatgcg aagatgagct gtcaccggtc
    12241 cgaagccgag tccatggcca gtactgcgac agcggaacgg gactacaacc cgcccgagct
    12301 catgcagccg agggcgccgg ccgcgacgcc ccgggaaatg aaaaacggcg cgcgggtgaa
    12361 ggccaacaac gtggcgccac agggcgtcta tctcccgaac tacaacatcc ttaccccgca
    12421 catgcgctcg ccggagtacg tgcagatgtc cacggcagcg gcgatcacgc tcggcatcat
    12481 gaggggccgg atgtaccgct gctcctgcac gcgctgcttg aatctcctgc tcacctaccc
    12541 ggaagggtgc cgcgcgaatt gtgcgtactg cggccttgcc cgccaccgcg aggccgagcg
    12601 cgactatgcc gaccgcaact tcatccgggt cgactggccg gcggtgccgc tcgacaccat
    12661 gatcgacatc gttgcgcgcg acggcacggc aagccccttc caccggatgt gcatctcgat
    12721 gatcacccac ccccgttccg acgcggacac ggtgacggtg ctcaggaagt ggaccgaccg
    12781 cgtcgatcca tcggcgatcc cggtttcgat cctctccaac cccacgacca tgacgcgcga
    12841 cgacgtcaag cgactgcgcg agctgggtgc ggacatcttc accgtcgcgc tggatgcggc
    12901 cacgccggaa atcttcgatc gcacgcgcgg caagggcgtc cagtcgccgc actcgtgggc
    12961 caagtactgg gaaatcctca tggacgcccg caacatcttc gggcccggga agttcggggc
    13021 gcacatcatc gtcggcatgg gcgagaccga gcatgacgtg ctctccctcg tccagcggct
    13081 ggtggacctc ggcggccaca gccacatgtt ctgcttcttt cccgagaagg gctcgctgat
    13141 ggaccacctg ccggcgacac cccgcgacca gtggcgccgc gtccagctcg gacgctacct
    13201 gatcgattat cgcggcgcgc gggtcgagcg gatgttgttc gacgggcagg gccgcgtcac
    13261 cggctacggc gttgcggcaa gcgaggtgga agcggttatc gaggaaggca tcgcgttccg
    13321 cacctcaggc tgccccggca agttccgcga cgacgtgtcc gcgtgcgacc gcccctacgg
    13381 cgactcgccc ccgagcgata tcgcgagcta tccatttcag cccggcaagg gcgaccggcg
    13441 caggatccgc cgccagctgc gcctggtcga aacaagggat tgacggcgtg gcggcggtct
    13501 ttcgcgtgat cgacaccggc atacgcgacg gccggcggca gatcgcgttc gaccaggcca
    13561 tgatcgacgc ccacaaggcg gacgccattc ccgactcgat ccgcttcctg cgctttcctc
    13621 ccacagcgtt gatcggccgc catcaggtgc tcgcgcgcga gatcaacctc gactactgcc
    13681 gggcgaacgg cgtcggcatc gtccgccgca tcaccggcgg cggcgcgatc tacttcgacc
    13741 cgggccagct cggctgggaa ctggtgttcc accgctccac tttgggaatc ggctcgctcg
    13801 ccgagttgac gcgcggcatc tgcgaggccg cagccgcagg gttgagccgc ctcggcgtca
    13861 gcgcgaatta ccggccgcgc aacgacatcg aggtggacgg gcgcaaggtg agcggcaccg
    13921 gcggattctt cgatgaggac acgctgttct accagggcac ggtgctcgtg gacatgaatc
    13981 ccgcggacat gatcgccgcg ctcaacgtcc cccaggcgaa actcgccaag cgttcgctcg
    14041 attcggccgc gcagcgcgtc gtgacgctga aggagctgct cggcgccgcg cccgccatcg
    14101 aatccgtcaa gctatcgctg ctcgcgggtt tcgcggagca gctcgggatc acccccgttc
    14161 ccggcgagat caccagccat gaggaagcgc tcgccgcgaa gaatcacgac gaggagatcg
    14221 gcaccgaggc gttcgtcgcc gggatcgacg atccgggcgc ggacgaaggc gtgctgtcgg
    14281 cgagccgcac cggcgccggc ggcacgatcc aggcgtacgt ccgtctcgag ggcgcacggc
    14341 gcgatcgcat ccgtgaagcg ctcatcaccg gcgatttctt cgtcaccccg ccgcgcacca
    14401 tcctggacct ggaggcgtcg ctccggaacg tcgccgttgc cgaggccggc gccacgatcg
    14461 agcgcttctt cgccgatacc aaggtcgggc tgctcaccgc ggcgccggcc gatttccgca
    14521 aggtcgtgga agacgccatt gcatcggcct aggggtttgt cggacttcgg tccgataaac
    14581 tcctaagagg ctgtttcaaa atgaacgaaa cagcccctgt tgtaggggag tatgagggga
    14641 gtatcccccc atattgttac cgtctctaat gcaaaaccgg cgtctggaaa aaacacaaaa
    14701 agaggaggaa tgaggctcat ctccgtcgtc cagcacacct ccgccgagta tctcggcctg
    14761 atcgaagacc atctcgaggg acgcgggatc cgcttcaagt acttccggcc gttcaccgaa
    14821 ggcggagggc tgcccgaccg cgatgcgatc cgcgacggcc tggtgctgct cggcgggggc
    14881 gtgtggggcg ccgcagggac gcgcgatgtt ccgacgctca agcaggagac ggggctcgcc
    14941 cacgccgcgc ttatgtacgg cagtcccgtg atcggcatcg ggctcggcgc gcagatcctc
    15001 gcgatcgcgg ccggcggccg ttccgcggac ggcccgctca ccttcgaggt gaccaccgcg
    15061 cgccggctcg acgcgtccgc cttgaacggc tacctgcccg agcagtaccc gctcgccatc
    15121 tacatgcgcg actggccggt tccgccggcg tatgccaggg tgctcgccga ggaccccaaa
    15181 gggcggcccg cgctctggca gatcggcgat tgcgcgttcg ggttcgccgg gcaccccggc
    15241 ctcaaggtcg ccatggtgga agacctgatc atggaattca ccgaagcgcc cgcggatccc
    15301 ggccccgaac tggcgaaact gcggtcggcg cagcgcgccc tggaggacgc cctcgtgccg
    15361 atcatgaccg gcctcgtgca gctcacgaag tggatgcagc cgcggggccc ggcagtctag
    15421 accccaccgc cagaagggag aagacgacgc gtgaaacggg cttgccttta ttagaattct
    15481 ctaatatatt gcttttgcct gcggcccgat gtcccgcacg ggatgggccg cggtccgccg
    15541 cggatgcccc aacccagcgg cggtgcacta gactgcgact ggaggggatg gccctatggc
    15601 cggcaagctc atcatcgtga tggccaacac cgatccgcgc aacggcgagg agctcggcgc
    15661 gccgatcttc caggcgaccg tggcggcggc catggaatac gaggtcgagg tcatctgcac
    15721 cgccaccgcg gggcgcctca tgaagaaggg cgtggccgag aagctccacg tgaaggaagg
    15781 ctcgccgaag tcggtctacg atttcatcaa ggacgcgcac gaggccggcg tcaagttcta
    15841 ctgctgctcc cccaacctcg atctgttcga catgaaggag agcgatctga tccccgagtg
    15901 ctcgggcatc atcggcggcg cccacctcat cgaggaagtg atggagggcg actgcaaggt
    15961 gatgacctac tgacgtgagg ccatggcgca cgcgacagcc acccgcgttc aggagcacat
    16021 cggcgacccg gtctccgaga agcccgcggt cgcgcgcgcg aagctcgagg agatcttcaa
    16081 ggacatccag tcggacctcc gttacgacca cgagctgaac gggtgcctga actgcggcat
    16141 ctgcacggcc acctgtcccg ccgcccacta ctacgacttc agcccgcgcg agatcgtcca
    16201 gctgctctgg accgaaaacc tcgagggcat ctacgacgcg atgcaggaaa agatctgggc
    16261 ctgcgcccag tgctacacct gcgccgcgcg ctgcccgttc ggcaattccc ccggcggcct
    16321 cgtcatgctg atgcgcgagg cggcgatcaa gcacggcatg gaatcggcaa agaacgtgct
    16381 gcgaccgttc agccgcgtcc tgctgaagct gatctcgacc ggcaaccagc tctcgccgga
    16441 catgatcaac cgcgatcatt tcgccgattg gggcccgaac gtctccaaga tcgacgcgcc
    16501 cctcaaggtg ctgcgcaagg cgatcccgat gcccacgctc aataccacgg tcaccgcgtg
    16561 ggaagtcaat ttgaagacct cggtcgagct ctacacgatc tgggaggaga ccggcgtgct
    16621 gaagcagctc gagaccgtcg atgagaacct gttcgacgtg atccaggaca tcatggacga
    16681 gaagcgcgag gactggaagg acttcctcgc ggagcaggaa gaagcgaagg acgattgagg
    16741 aggcagtatg gacaagcaaa cccccggaaa cgaacgcttc accggccacg gatccgagtg
    16801 gcgcgacgcg aatctctcgc ccaccgaggc gcggatggcg accgcgtggg tcgagcagaa
    16861 gatcgaccgg cgctccatgc tcaccaacaa ggagcgcgtg caggacgtcc gcgacatcat
    16921 gtggcagctg gagaaggacg gcgagatcgt cgttcatcga gtctccgacg agcacgagcc
    16981 ggtgatggtc aaaacgctct atggctggga caagaagatt ccaacgcggc gactctggca
    17041 ccataagtcg tgcgggcagt gcggcaacat cccggggtat ccggcatcgc tcttgtggct
    17101 gatgaaccgg ctcggcatcg agtacctgga tgaaaccgac cagacctcgt gcaccgcgtg
    17161 gaactaccac ggctcgggca tcggcaacct ggaaagcctg gccgcggtgt tcctgcgcaa
    17221 cttccatcag gcctatgtgg ccgcgaaggc gcaaggattg cccgaggcct atttctatcc
    17281 gctcgtgcat tgcggcacct cgttcggcaa ctacaaggag gtgcgcagct atctgctgct
    17341 atcggcgaag ctgcgtgagc gcgtgcggca aagtctcggc aagctcgggc ggctggtgga
    17401 cggcaagctc ctcatccccg aggaggtcgt ccactactcg gagtgggtcc acgtgatgcg
    17461 ccatcgcatc gcgagcctgc aggaaatcga c
//
LOCUS       DBNGWZ010000451         4474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_433188, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000451 DBNGWZ010000000
VERSION     DBNGWZ010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_433188"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          834 a         1407 c         1447 g          786 t
ORIGIN      
        1 tgcttgagca cgtacccgac cgccccggcg cggatggcgg cgaagagcat ttcgtcctca
       61 gcaaagctgg tgagcatgat gattttgatg ccggggagct ggttgttgat ttcacggcag
      121 gcgtcaatgc cgctggagcc gccgaggcgg atgtccatga cgatcacgtc cggcttgaag
      181 gcgaggcttt tggtcaaagc ctcgggcgcg gtggcggcct cggccacgac ttccatctcg
      241 ggttggcgct cgatgagcgc cttcaacccg agacgcacca cctcgtggtc gtccaccagc
      301 atcacccgtt gtcgccgcat gctcgtcatt ttacccgctc acgccgtcat tcgacaagcg
      361 cgaaaacgct cagattggcg tgggttaacg accggattgg cgcccgttgc cagcaggcgg
      421 cgagtgtttt acaatcacca gtgatggaaa ccgccccgga aacgccacaa acacctcccg
      481 cggctgtggc gatcaccaat gatcaattga tagcgctgcg cgaagcggcg cttgccatct
      541 cgtccgatct ttcgctgacc gaaacgctga agcgcgtcgt cgccgtcgcc gcggagctgg
      601 tccacgcgcg ctacgcggcg ttgggggtgc cggacgagtc cggcgaactg ctggccgagt
      661 tcgtcaccac cgggctgtcg ccagaggccg aggcgcgcat cagccaccgt ccgcgcggcc
      721 acggcctgct ggggctgatc atgcgcgagg gccggccgct gcgcctgcgc gatttgaccc
      781 agcacctgaa gtcggtggga ttcccgccga atcacccgcg catgacgagc tttctggggg
      841 tgcctatcct ctttcagggc aagtcggtgg gcaatcttta tctcaccgac aagcggggcg
      901 cggaggagtt cagcgaaacg gatcaaacgc tcgtcgaatt gctcgcgacg cacgcggcca
      961 acgccgtcca caatgcccac ctgtatcaga ccactgtggc gcacggcgag gaattggagc
     1021 agcgcaaccg cgaactggcc gcactcaacg cggtcgccat cgccacgagc cagcatctcg
     1081 acctgaatcg ggtcctgagc gaggcgctcg atcacgcgtt ggccatctcc ggcgcggagg
     1141 cgggcgaaat ctttctgctc gaccaggcca gcggcgacct ggtgctggcc ttgcatcggg
     1201 gaacatttcc ggaaagcttt cggaccattc gccgcttccg gcgcggcgag ggtttccccg
     1261 gcgaagtcgc ggtgaccggg cagcctctgg tcacccacaa cgtcgctcgc gacctgaact
     1321 ttctgcgcga tcaggtcgtc caggcgggct tcacctcgtt tgcctgcctg ccactcatcg
     1381 ccaaaggcaa ggtggtcggc acgctggaca tggcctcgca cgacccccag accttcgacg
     1441 agagggacct gagtctgctg acggccatct gccatcagat tggcgtcgcc gtcgaaaacg
     1501 cccggctgta cgggcaggtg cagcagctcc gcgtagtcga ggagcgccag cgcatcggca
     1561 tggacttgca cgacggcgtc atccaatcca tctacgccgt ggggctgacg ctggaataca
     1621 tcaccagtca actggcagac ggggacgtta cgggcgcgag cgagcgactg cggggcgcga
     1681 ttgaggcgct caacgccacc atccgcgata tccgcgcgta catcctcgac ctgcgcccgc
     1741 gccgattcga gggcgacgat ctggccctcg gcctggaacg gctgctggcc gagttcaaag
     1801 cgaatacgct gatgcaggtg gatttcgccg ccgacgcgga cgcggaccag cagctggtgc
     1861 cggaagcgcg cctcgcgctc tttcacatcg cgcaggaagc gctgagcaac gccgcccggc
     1921 acagccgcgc gtcgcgggtg agggtcagat tattggatgg cggggccgcc gtgacgcttt
     1981 cgcttaagga caacgggcgc ggcttcgacc ccgagcgggc cgagcggcgc gtgggccacg
     2041 gtctgctgaa catgcgcgac cgggcgtcgg ccattggggg cgagttaacg attgaaagcc
     2101 ccgatcgtca ggggactgaa gtgcgtgtgc gcgttcccaa acgcaccggg atttaatact
     2161 tctcccggca cgttcagggg tctgccgaac caggccggct aggactgcac gggaacagcc
     2221 accggatggc cgttcgatct catcaagtgc ccaaacttcg tatcggccaa ccgccggacc
     2281 aaatcggcct gagcgtcaca ccatacatct cgcaccgtgc aatcgtcccc gagggggcat
     2341 tggcccgggt cggtggtgca gttgttgagc atcaccgggc cgtcaatcgc ttcgacgatt
     2401 tccagcaggc tgatttcctg cgggggacgg gcgagcgtta cgccgccacg cgcgccgcgg
     2461 acggcgcgca ggatgccggc ggccgagagc tgggagataa tcttggccag gaacgtcgcc
     2521 gggatgcgct gttcgtccgc gacctgggcc gtggacgcgc gtgcgcccgg gccaagctcg
     2581 gcgaggtaaa gaactgcacg aaccgcgtaa tctgcttgtc gtgtgatttg cacaacaacc
     2641 tccattcttg cgcaaggcga taaaaccagt ctggattaac tgtacggcat gtcgcccttt
     2701 ggcgggcagt gacgaatatc actctcagag aggacagtta tcctcttcac aatccaggtc
     2761 aggctggctt gggagcgcaa gcccaatcgc agcccagtgg agccgtttat agtcaacttg
     2821 aggccaatgt tcctgaacaa ccgtcacgta accatttcga acaggctgtg ggcggattcc
     2881 gcgtgggtga ggtgaagcag ggtggggggc gctggcgatc acaggcttgt ggtggccggt
     2941 gggaagtgac gagaagctga gcgcgaggcg tcgccaggtc gcttctttct ccggcggcgg
     3001 gagcgccgaa gccttgcgcg gccttatgac atttatcggc acaggagagg atagccttca
     3061 ctgagcggag ggggaagctg agattagact gtacacatgt ccgggccgtt gcggattctg
     3121 atcattgacg atcacgacga cgttcggcgc gcgctgacgg cgcggttgag ctccgcgccg
     3181 ggaatggcgg tcatcgggga aacggccgac gccgaagatg gtctcttgaa aacgcggaca
     3241 ctccgtccgg acgtggtggt ggtggaaacc aagcggatgg acgggcgcgg gctggagatc
     3301 gtcagctgga tcgcccagag cgggatcggg gcgcgcgtca tcgtcttgac gtcgtacccc
     3361 agcgagtggg aacggtgggc ggctcaccgt gcaggcgccg cgcggtatct gctcaaagac
     3421 atcgcctccc cgctgctcat cgagcacatt cgcagcgccg ccgcccatct ctcgtctcac
     3481 gactactatc atccacaccc gacctcaacc tgatcctcgc gaaacagccc tcgatccagg
     3541 taaaaggggc ggcgcggggg gcaggggaaa gaccttcggg gcagcaggtt gcgcctgcct
     3601 gccgacggct tttgggctat aatacgccca tccttccgcc cacccggagt ggttatatgc
     3661 gcctcatcac ccgcgccgac tgggatggcc tggtctgtgc cgttctcctc tctaccgtcg
     3721 agcacattca ggcgatccag ttcgcttacc ccaaggacat ccaggacggg aaagtcaacg
     3781 tccctgagaa ttcggtcatc gccaacctgc cctatcaccc gaactgcacg atctggttcg
     3841 accatcacat ctccgaagag gaattgggcg cgcccatggc ggccttcacg ggcatcaagg
     3901 gcaagttcgg gctgtcaccc tcggcggcgc ggctggtcta cgagtattac ggcggcgacg
     3961 gaaacgagaa gctggcgcgg tacaaaacgc tggtggaggc gaccgaccgg gtggatagcg
     4021 cacagttgac gatggaggac gtgacccatc cgtccgacta cgtcctgctc gcctacacga
     4081 ttgacccgcg caccggcctg aaggacatcg aactgcagat gtacttcatc atgatgattg
     4141 actggctgaa gagcaagacg ccggaagaca tcctggagat tccggaagtg aaaatgatgg
     4201 tggaacgcct gcgctccgac gaccaggctt tccgcgaggc gctgctccag ttctcccggc
     4261 tcgagggcaa cgtcgtcgtc accgacttcc gaggcgtggc cgtcccggcg ggcaaccggt
     4321 tcatcatcta caccctcttc ccgcaggcca acgtctcggc gcggctgttc gacggcaagg
     4381 acgggacgtt cagcaccatc tcgctcgggc acagcatctt caaccggacg tgcaagacga
     4441 acgtgggcaa gctgctggcc gagtacggcg gcgg
//
LOCUS       DBNGWZ010000452         4391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3207155, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000452 DBNGWZ010000000
VERSION     DBNGWZ010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3207155"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="AC16CX_13875"
     CDS             <1..806
                     /locus_tag="AC16CX_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3013630.1"
                     /translation="KDPEIGAVVLATPHSLHWKQIVQAAKAGKHVFVEKPLTLTRKTA
                     EKAVEACRAAGVTLGVGFNRRHAPAFVDMMRRIRAGEIGDVLHVEATHSGPSGYRLKG
                     GNWRATRAEAPAGGMTARGIHTLDGMIQIAGLVTSVFAFSEKRKLPPEVEIDDTTSML
                     LRFASGATGSQSTVFITGDIYCTRVFGSQGWLEMRGDTELVARGLDGAPQSFTFPAVD
                     KERAELEAFADAVEAKQPFLVPPEQAVNEIAVLEAIVASAARGKPVKIN"
     gene            1284..2168
                     /locus_tag="AC16CX_13880"
     CDS             1284..2168
                     /locus_tag="AC16CX_13880"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR3013631.1"
                     /translation="MRFDLTTLRLFVAVIDHGSITKAAEQEHIVASAVSRRLSDLEMQ
                     LGIPLLMRQHAGVVATRAGEALAQHARDVIHAVDRIPSALSTAAGDDSLTLRVWANMT
                     ATVGYLLDDLNVFLSRHPQVKIRLEERRSLPTIDAVVDGSADLGVIGYFQPADRLQVR
                     PYRRVPLMLVVPPAHPFSGRKEIRFAEVLEFDLITLMEGTAIHSWTVEAGARIARKPR
                     LVMEVTSYESMRKMVQAGLGIAVIPAPNIVPYIDLFGLRAIPLSDSWAAMDLNLIFNE
                     ARSDVPGLAELIEHLSRA"
     gene            2295..3254
                     /locus_tag="AC16CX_13885"
     CDS             2295..3254
                     /locus_tag="AC16CX_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013632.1"
                     /translation="MSTHRFALTLLALAAATASAQTYPNKAIRIVVPYPPGGGADMTA
                     RPIAQKLSERWGQPVVIDNRGGASGMIGADIVAKAPPDGYTLMVSASAEAALNVALFP
                     KMPYDPVKDFAPITLAIVIPMALVVHPSVPIKSVKEYIAIARAKPGSMTYGSAGAGST
                     HHFAGEWLKMLTKIDIVHVPYKGMGPAITDLLGGHLPSGFATLLPALQHVKTGKLRAL
                     AVTTQKRAPLLPDVPALAETLPGFNVTQWNAVWAPAATPKEVLDKLSAEITRIVQSPD
                     YKARMVEQAAEAIGDPPSELAAFQKAEIDKYRKIAQQANIKVD"
     gene            3281..4063
                     /locus_tag="AC16CX_13890"
     CDS             3281..4063
                     /locus_tag="AC16CX_13890"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HIR3013633.1"
                     /translation="MDDVVTRPKEAYHQRFRAEMERWFPTADWRLQDRIALACRMLHR
                     DGHDSALAGQISARGKQPGTYWTLSFGLGLDEARSSNVLLVDDDLSVLAGDGMANPAN
                     RFHLWIYRARPEVNAIVHTHAPYASALSLLAEGLVVAHMDTCVLYESCAYLAEWPGVP
                     IGDEEGRIISEALGDKPAVLLAHHGLLTATESIERAAVLAFYLERAAKMHLRARAVGP
                     IRTVNPELAREARGYRGSPQYISATFNYLARCVLRETPECLT"
BASE COUNT          872 a         1388 c         1395 g          736 t
ORIGIN      
        1 tgaaggaccc ggagatcggc gccgtggtgc tcgcgacccc gcactcgctg cactggaagc
       61 agatcgtcca ggcggcgaag gcgggcaagc acgtcttcgt cgagaagccg ctcacgctca
      121 cgcgcaaaac cgccgagaag gcggtggaag cgtgccgtgc cgcgggcgtc acgctcgggg
      181 tgggattcaa ccgccgccac gctcccgcgt tcgtggacat gatgcgccgc atccgcgccg
      241 gcgagatcgg cgacgtgctg cacgtcgagg cgacacattc gggcccgagc ggctaccggc
      301 tgaagggggg caactggcgc gccactcgtg cggaggcccc ggcgggcggg atgacggcgc
      361 gcggcatcca cacgctcgac ggcatgatcc agatcgcggg actggtgaca agcgtcttcg
      421 cgttcagcga gaagcgcaag ctgccgcccg aagtggaaat cgacgacacc acctcgatgt
      481 tgttgcggtt cgcgagcggc gcgaccggca gccaaagcac ggttttcata acgggcgaca
      541 tctactgcac gcgggtcttc gggtcccagg gctggctcga gatgcgcggc gacaccgagc
      601 tcgtcgcgcg cggcctcgac ggcgccccgc agtcgttcac cttcccggcc gtggacaagg
      661 agcgcgccga gctcgaggcg tttgccgacg cggtggaggc caagcagccc ttcctggtgc
      721 cgcccgagca ggccgtcaac gagatcgcgg tgctggaggc gatcgtcgcc tcggcggcgc
      781 gcggcaagcc ggtcaagatc aattgaaccg ccaagacgcc aaggagaaca aagtttgaac
      841 cactaaggac acaaaggaca cgaaggaaca acgcttcccc gaatcctcga tcccgaatcc
      901 tgcctttctg aagccgccga tgaacgcaca taaacgcggc tacgaccaac cccgaaaagc
      961 cttagccaca gaggacacag agttcacaga gaagaaacaa accagacgac aaattggttc
     1021 tggtccttca actgtagtag ctcagacttg atctgacaga cagtgtctga ccaggtcggc
     1081 tctaatgcat ttattaaggg ctgaaaacgg ctaaaagcag ccgttcgatg agggggccac
     1141 gaacaccggg tgggcgctta atagcggggt ttctggtagc ttattgtggg tctgacctgg
     1201 tcagacccgc tcggagtaag tttccctgtg gccgaaatct tatcctgacc agctctgacc
     1261 ccatcaccca ttccctatcg cccatgcgct tcgacctgac gactctgcgc ctgttcgtgg
     1321 cggtcatcga ccacgggagc atcacgaagg ccgccgagca ggagcacatc gtggcgtcag
     1381 cggtgagcag acgtctcagt gatctcgaga tgcagctcgg aataccgctg ctgatgcggc
     1441 agcacgcggg cgtcgtcgcc acgcgtgcgg gcgaagcgct cgcgcaacat gctcgcgacg
     1501 tcatccatgc ggtcgatcgc atcccgagcg cgttgagcac ggcggcaggc gacgactcgt
     1561 tgacgctgcg cgtgtgggcg aacatgacgg cgactgtcgg ctacttgctg gacgatttga
     1621 atgtcttcct ctcccgacac ccgcaggtca agattcggct ggaggagagg cggagcctgc
     1681 cgactatcga tgcggtggtc gacggcagcg ctgaccttgg cgtgatcgga tacttccagc
     1741 cggcggaccg gctgcaggtg cgaccgtacc gaagagtgcc gctcatgctc gtcgtgcccc
     1801 ctgcgcaccc gttctccgga cgaaaagaga tccgcttcgc ggaagtcctc gagttcgacc
     1861 tgatcacgct gatggaggga acagcgatcc acagctggac agtcgaggcg ggcgcccgta
     1921 tcgcccgcaa gccgcgactc gtaatggagg tcactagcta cgagtcgatg cgcaagatgg
     1981 tgcaggccgg cctcggaatc gcggtgatcc ctgcgccgaa cattgttcct tatatagatc
     2041 tattcggact tcgcgccatc ccactctccg acagctgggc cgccatggat ctcaatctga
     2101 tcttcaatga ggcccggagc gacgtgccag gcctcgcaga gcttatcgag catctttcgc
     2161 gcgcgtgagt ggccatcgcg agccgcgatg gccgccatgc ccaaagatta gttctccgcc
     2221 agccgggatc ctcgtatgct ggcctcggtt cgatcgcggt gatcggaccg atactaacga
     2281 ccgaggaggc tactatgagt acccacagat tcgctctgac actgctcgcg ctggcagccg
     2341 cgacggcgtc ggcacagacc tacccgaaca aggcgattcg catcgtcgtg ccgtacccgc
     2401 cgggcggcgg cgccgacatg accgcgcggc cgatcgcgca gaagctcagc gaacgctggg
     2461 gccagccggt cgtcatcgac aaccgcggcg gcgcgagcgg catgataggc gcggacatcg
     2521 tcgccaaggc gccgcccgac ggctataccc tcatggtcag cgcctcggcc gaagccgcgc
     2581 tcaatgtcgc gctcttcccg aagatgccgt acgacccggt caaggatttc gcgccgatca
     2641 cgctggcgat tgtgatcccg atggcgctgg tcgtgcatcc gtcggtaccc atcaagtcgg
     2701 tgaaggaata catcgcgatc gcaagggcga agcccgggtc gatgacgtac ggttcggccg
     2761 gcgccggctc cacccatcac ttcgccggcg agtggctgaa aatgctgacc aagatcgaca
     2821 tcgtgcacgt gccgtacaag ggcatgggcc cggcgatcac cgatctgctc ggcgggcatc
     2881 taccaagcgg gtttgccacg ctgctgccgg cgctgcagca tgtgaagacc ggcaagttgc
     2941 gcgccctggc ggtgacgacg caaaagcgcg cgccgctgct gccggacgtg ccggcgctcg
     3001 ccgagacgct gcccggattc aacgtcaccc agtggaacgc cgtgtgggcg ccggccgcga
     3061 cgccgaagga agtgctcgac aagctgagcg cggaaatcac gcgcatcgtg cagtcgcccg
     3121 actacaaggc gcgcatggtg gagcaggccg ccgaggccat cggcgatccg ccatcagaac
     3181 tcgcggcctt tcagaaggcg gaaatcgaca agtaccgcaa gatcgcgcag caggcgaaca
     3241 tcaaggtcga ctgaactgca accataaaga ggatactcag atggacgacg ttgttacgcg
     3301 gccgaaagag gcttatcacc agaggttccg cgcagagatg gagcgttggt tcccgactgc
     3361 ggactggcgg ctgcaggatc ggattgctct cgcctgccgc atgcttcacc gcgacggcca
     3421 cgactcggcg ctcgcggggc agatctcagc acgcggcaag cagcccggaa cctactggac
     3481 gctcagcttc gggttgggcc ttgacgaagc gcgttcttcg aacgtgctgc tcgtcgacga
     3541 cgacctaagc gtgctcgccg gagatggaat ggcgaacccg gcaaaccgct ttcatctatg
     3601 gatctaccgc gcccggcccg aagtgaatgc gatcgtgcat acccatgcgc cgtacgcctc
     3661 agcgctgtcg ctgctcgcag aaggactcgt cgtcgcccac atggacactt gcgtgctgta
     3721 tgagagctgc gcgtatctgg cggaatggcc tggcgtgccg atcggcgacg aggagggcag
     3781 aataatttcc gaggccctgg gcgataagcc ggctgtgctg ctcgcccacc acggcctgct
     3841 caccgcgacc gaaagcatcg aacgcgctgc cgtccttgcg ttctacctgg agcgcgcagc
     3901 gaagatgcac ctgcgcgctc gagcggtcgg cccgatccgc acagtcaacc cggagcttgc
     3961 ccgcgaggcg cgcggttatc ggggcagccc tcagtacatt tcggcaacgt tcaattatct
     4021 cgcgcgttgc gttctgcgcg agacgcccga atgcctcact tgaaaccagt cgtacaccgt
     4081 gtccgagtta tgcgctgggt cgacaacagc ctcgatcacc gtagacccat cgacttgaaa
     4141 tgcgtgcgcg agctcgcgtt cgagctgagc cgggctccgc accccaacgg cgggcacacc
     4201 gagataatgc gcaggcgatg cgtgcgtatc gaacaaatta aaactcgtgg aataggccgc
     4261 tctcggctgc ggtttcaatc ggtcatcgca acattctatt ctctgacgag agagggagga
     4321 tgttgcaaat ggagcacaga ccgaggatct attacacgga agctcagaag gcattgatgt
     4381 gggatcgctg g
//
LOCUS       DBNGWZ010000453         4627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_685436, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000453 DBNGWZ010000000
VERSION     DBNGWZ010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_685436"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="AC16CX_13895"
     CDS             <1..1129
                     /locus_tag="AC16CX_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3013634.1"
                     /translation="AQRFYSDSGHVLRVDRAMNQQFDLKQSKILVVDDESGIRRLLGM
                     ELRAAGYDDVTLCSDPRKVVELYRKNRYELILLDLRMPEMDGYQVMSALKEIETDGYL
                     PVLVITAGPEEKMKALRSGAKDFITKPFERPELLTRVKNLLEVRLLYRKLRDYSGDLE
                     YKVQERTARLTGSYHETLRTMTRAAEYRDDNTGAHVRRISFYSRELAERLGMDGEFCE
                     RIFHASPMHDIGKIGIPDSILMKKGPLTPRQWDVMRTHAIIGARMLMDGESPYLKMGA
                     QIAAAHHERWDGTGYPNQLKGEETPLAARIMAICDVYDALRSARPYKPAFEHDKAVKI
                     IIKGDNRTKPRHFDPAVLDAFQRGAAVCRDIYAANADLPQG"
     gene            complement(1199..2071)
                     /locus_tag="AC16CX_13900"
     CDS             complement(1199..2071)
                     /locus_tag="AC16CX_13900"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013635.1"
                     /translation="MARIAFIGLGVMGYPMAGHLKKKGGHEVTVFNRTTGKAAGWVKE
                     HGGASAPTPREAAREADFVFCCVGNDDDVRSVVYGENGALAGMKKGAILIDHTTASAI
                     LARELDARTRELGLGFLDAPVSGGQAGAQNGQLGIMVGGEPATFERARPVLDLYARSC
                     ALIGPAGSGQLTKMVNQICVVGVIQGLAEGLDFGRRAGLDMDKVLSVITKGAATSWQM
                     ENRSKTMLEGRFDFGFASDWMRKDLSICLAQARAIGARLPVIALIEQFYAQVQARGGG
                     RWDSSSLINLLAKD"
     gene            2254..3306
                     /gene="recA"
                     /locus_tag="AC16CX_13905"
     CDS             2254..3306
                     /gene="recA"
                     /locus_tag="AC16CX_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3013636.1"
                     /translation="MDDNKAKALAAALSQIEKQFGKGSIMRLGESEISKDIMVVSTGS
                     LGLDLALGIGGLPRGRVVEIYGPEASGKTTLALSVIAQMQKLGGTAAFIDAEHALDPQ
                     YAGKLGVDVDQLLISQPDNGEQALEIADMLVRSSSVDVVAIDSVAALVPKAEIEGEMG
                     EPQMGLQARLMSQALRKLTANIKRSNTLVVFINQIRMKIGVMFGNPETTTGGNALKFY
                     ASVRIDIRRIGSIKKGEEVIGSETRAKVVKNKVAPPFRQAEFDILYGEGISREGEIVE
                     LGTIHRILDKTGAWYTYGKERIGQGKDNARDYLKEHPDIAREIEAKIRAAAGAAQPAA
                     VLPKTEPRAEARKAAH"
     gene            3452..3895
                     /gene="recX"
                     /locus_tag="AC16CX_13910"
     CDS             3452..3895
                     /gene="recX"
                     /locus_tag="AC16CX_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HIR3013637.1"
                     /translation="MAESLRERALKLLARREHSRAELARKLSAHAEDPDEIGRVLDDF
                     ERRGWLSERRVVEQLMHAKRSRFGARRIEQELLRKGISGDAVAAALTELRSGELEAAR
                     EVWRRKFGGRLPRAAAERARQVRFLQGRGFELDVIMKVIKNKDEG"
     gene            3906..4295
                     /locus_tag="AC16CX_13915"
     CDS             3906..4295
                     /locus_tag="AC16CX_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013638.1"
                     /translation="MKEAALATSHESRVASHARAAVLALAAALALAGCDYLPFGYTPV
                     KDIHATPAQFEGKEVKLKGRAKGVTKLLGLKSYTLQDETGEISVVTEGQLPAENSDVA
                     LKGTVKSAMIVGGKSVGLRVEETQRLR"
     gene            4401..>4627
                     /locus_tag="AC16CX_13920"
     CDS             4401..>4627
                     /locus_tag="AC16CX_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3013639.1"
                     /translation="MKSSEIRSKFLEYFADRGHAVVASSPLVPANDPTLLFTNSGMVQ
                     FKDVFLGQDKRPYARAATAQRCVRAGGKHNDL"
BASE COUNT          924 a         1399 c         1507 g          797 t
ORIGIN      
        1 cgcgcagcga ttttattccg acagcggcca cgttctccgg gtggaccggg cgatgaatca
       61 acaattcgat ttgaaacaat cgaaaattct ggttgtcgat gacgagtccg ggatcaggcg
      121 gctgctcggc atggagctgc gcgctgcggg ctatgatgac gtgacgcttt gcagtgatcc
      181 cagaaaagtc gtggagcttt acaggaagaa ccgttacgag ctgatcctgc tcgacctccg
      241 catgcccgag atggacggtt accaggtgat gtcggcgctc aaagaaatag aaaccgacgg
      301 ttacctgccc gtgctggtga ttaccgccgg gccggaagaa aaaatgaaag ctctgcggtc
      361 aggcgccaag gatttcatca ccaagccatt cgaacgcccc gaactgctca cccgtgtgaa
      421 aaacctgctg gaggtccggt tgctgtatcg caagctgcgc gactacagcg gggatctcga
      481 atacaaagtt caggagcgca cggcacgcct taccggcagc tatcatgaaa cgctgcggac
      541 catgacgcgt gcggcggagt accgtgacga caacaccggc gcacatgtgc ggcgaatcag
      601 cttttactcg cgggagctcg ccgagcggct cggcatggat ggcgaatttt gcgagcggat
      661 tttccacgcg agcccgatgc acgatatcgg caaaatcggt atcccggatt cgatcctgat
      721 gaaaaagggg ccgctcacgc cccggcagtg ggatgtgatg cggacgcacg cgataatcgg
      781 ggcgcggatg ctcatggatg gcgaatcgcc ttatctgaag atgggcgctc aaattgcggc
      841 tgcgcatcac gagcgctggg acggcaccgg ttatccgaat cagttgaaag gcgaagagac
      901 accgcttgcg gcacggataa tggcgatatg cgatgtgtac gacgcgctgc gttcagcgcg
      961 tccttacaag cctgcgttcg aacacgacaa agctgtgaag atcatcatca agggcgataa
     1021 ccgaaccaag ccgagacatt tcgatcccgc cgtcctcgat gcattccagc gtggcgcggc
     1081 agtgtgtcgc gacatctacg cagccaatgc cgacttaccg caaggctgat ccgcgatacg
     1141 tcgttttcct gaaagacacg ccgcctttcc cgcgcgctgc gctccatccg atttctgatc
     1201 aatccttcgc cagcagattg atcaggctcg aagagtccca ccgcccgccg ccgcgcgcct
     1261 gcacctgggc gtagaactgc tcgatcaacg cgatcaccgg cagccgcgcg ccgatcgccc
     1321 gggcctgggc gaggcagatg ctgaggtcct tgcgcatcca gtccgatgca aagccgaaat
     1381 cgaacctgcc ctcgagcatg gttttcgacc ggttctccat ctgccaggat gtcgcggcgc
     1441 ccttcgtgat taccgacagc accttgtcca tgtcgaggcc ggcgcgccgg ccgaagtcga
     1501 ggccttcggc gagtccctga atcacgccga ctacgcagat ctggttgacc atcttggtga
     1561 gctgtcccga tcccgcgggt ccgatcaggg cgcagcttct ggcgtagagg tcgagtacgg
     1621 gcctggcgcg ctcgaacgtt gcaggctcgc caccgaccat gatgccgagc tggccgtttt
     1681 gcgctcccgc ttgcccgccg gataccggcg cgtcgaggaa ccccaagccc agctcccgcg
     1741 tccgggcgtc gagctcgcgc gcgaggatgg ccgaggctgt ggtgtggtcg atgaggatcg
     1801 cccccttttt catgccggcc agcgcgccgt tttcgccgta gaccacagaa cgcacatcgt
     1861 cgtcgtttcc cacgcagcag aacacaaagt ctgcctcgcg cgctgcctcg cgtggggtgg
     1921 gggcgctggc gccgccatgt tctttgaccc agccagcggc cttcccggtg gtgcgattga
     1981 agacggtaac ctcatggccg ccctttttct tcaggtgccc ggccatcggg tagcccatga
     2041 cgcccaatcc gatgaatgca atcctggcca tgcgcgcatc tcccatctcg agaaaaaagc
     2101 aatatggtaa ggatgttgga acactttgcc tatgcgacga ctgaggtccg tgggcgcgcg
     2161 tcgtcaactg agctttcgcg ccgcgcgtta ttcccctgtc gggcgatgcg agtggtttag
     2221 aatagacgcc aatgaacgac aggggaatcg cacatggatg acaataaagc caaggcgctc
     2281 gccgcggcgc tgtcccagat cgagaagcag ttcggcaagg gctcgatcat gcgcctcggc
     2341 gagtccgaaa tatcgaagga catcatggtc gtgtccaccg gctcgctcgg gcttgacctc
     2401 gcgctcggca tcggcgggct gccgcgcggg cgggtggtgg agatctacgg gccggaggcc
     2461 tcgggcaaga ccacgctcgc gctctcggtg atcgcccaga tgcagaaatt gggtggcacc
     2521 gccgccttca tcgacgccga gcacgcgctc gaccctcagt acgcgggcaa gctcggggtc
     2581 gatgtcgacc agttgctgat ttcccagccc gacaacggcg agcaggcgct cgagatcgcc
     2641 gacatgctgg tgcgatcgag ctcggtggac gtggtggcga tcgattcggt ggcagcgctg
     2701 gtgcccaagg ccgagatcga aggcgagatg ggcgagccgc agatgggcct gcaggcccgg
     2761 ctcatgagcc aggcgctgcg caagcttacc gccaacatca agcgctccaa cacgctggtg
     2821 gttttcatca accagatccg catgaaaatc ggcgtcatgt tcggcaaccc ggagaccacc
     2881 accggcggca acgcgctcaa gttctacgcc tctgtgcgca tcgacatccg ccgcatcggc
     2941 tcgatcaaga agggcgagga ggtgatcggc tccgagaccc gcgcgaaggt ggtgaagaac
     3001 aaggtggcgc cgccgttccg ccaggccgag ttcgacatcc tctacggcga gggcatctcg
     3061 cgcgagggcg agatcgtcga gctcgggacg atccaccgca tcctcgacaa gaccggtgcc
     3121 tggtacacct acggcaagga gcgcatcggg cagggcaagg acaacgcgcg tgactacctc
     3181 aaggagcacc ccgacatcgc ccgggagatc gaggccaaga tccgtgcagc ggcgggcgcc
     3241 gcccaaccgg ctgcagtgct acccaagacg gagcccaggg cggaggcgcg gaaggccgcc
     3301 cactaacgaa ggatgaaggc ggaaggcgga aggatgacct gccaacggct ccgcctgaag
     3361 gatgaaggcg gaaggatgat ggctgacctt gttcccagcc tccgcactgc gggggtcatc
     3421 cttcatccct catccttcat ccttccgggc gatggctgaa agcctgcggg agcgggcgct
     3481 gaagctgctc gcgcggcggg agcattcgcg agcagagctg gcgcgcaagc tctcggctca
     3541 tgccgaggac cctgacgaaa tcgggcgggt gctcgacgat ttcgagcgac gcggctggct
     3601 gtccgagcgc cgcgtggtgg agcagctcat gcatgccaaa cggtcccgct tcggggcgcg
     3661 tcgcatcgag caggagttgc tgcggaaggg catatcaggg gatgcggtag ccgcggcgct
     3721 gacggagctc aggagcggag aactggaagc tgcgcgcgag gtgtggcgca ggaagttcgg
     3781 tggccgcctg ccgcgcgcgg ccgccgagcg cgcgcgccaa gtgcgctttc tgcaggggcg
     3841 cggcttcgag ctcgacgtga tcatgaaggt catcaagaac aaggatgaag gatgaaggag
     3901 gaaggatgaa ggaagcagcg ttagcaacca gtcacgagtc acgagtcgcg agtcacgcgc
     3961 gagcggcagt gctggcgctg gccgccgcgc tggcgctcgc cggctgcgac tatctcccgt
     4021 tcggctatac gccggtcaag gacatccacg ccaccccggc ccagttcgag ggcaaggaag
     4081 tcaagctcaa gggcagggcg aagggggtga cgaagctgct ggggctcaag tcgtacacgc
     4141 tgcaggacga gacgggggaa atctcggtgg tgaccgaagg gcagcttccc gcggaaaatt
     4201 ccgacgtggc gctcaagggc acggtgaaaa gcgcgatgat cgtgggcggc aaatcggtgg
     4261 ggctgcgcgt ggaagaaacg cagcgtttgc gctaacgttg cggcgattga gtgattaggt
     4321 gattaagtga ttgagttgag cgcggtcctc tatattctga tttacctggt cactcagtca
     4381 cttagtcact cagtcacggt atgaagagca gcgagatccg cagcaagttc ctcgaatatt
     4441 tcgccgacag aggccacgcc gtcgtggcct cgagccctct ggtgccggcg aacgacccga
     4501 cgctgctgtt caccaattcc ggcatggtgc agttcaagga cgtgttcctc gggcaggaca
     4561 agcgtcccta cgcgcgcgcg gccaccgccc aacgctgcgt gcgcgccggc ggcaagcaca
     4621 acgatct
//
LOCUS       DBNGWZ010000454         7069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1067149, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000454 DBNGWZ010000000
VERSION     DBNGWZ010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1067149"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="ychF"
                     /locus_tag="AC16CX_13925"
     CDS             complement(<1..827)
                     /gene="ychF"
                     /locus_tag="AC16CX_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR3013640.1"
                     /translation="MSLRCGIVGLPNVGKSTLFNALTRAAIPAENYPFCTIEPNVGVV
                     PVPDPRLARIAEIARPAKVTPAVVEFVDIAGLVAGASKGEGLGNQFLAHIRETDGIID
                     LVRCFEDENVVHVTGRIDPVADIETVQLELALADLATVDKVLDRVAPKARSGDKEAIR
                     FEGVLARARARLDQGKPVRGLGLEAEELKLLQPLCLLTTKPAIYVANVGEHGFLNNPL
                     LERVREFAAKEGAPVVAICAAIEAEMAGMGEEDKKAFLADLGLEEPGLDRVVHAAYDL
                     "
     gene            complement(824..1585)
                     /gene="pth"
                     /locus_tag="AC16CX_13930"
     CDS             complement(824..1585)
                     /gene="pth"
                     /locus_tag="AC16CX_13930"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3013641.1"
                     /translation="MKLIVGLGNPGDRHQGTRHNAGFWLVERFAAQTGTVLRKDAKFQ
                     ALVGRHEASGAWLLLPQNYMNASGRPVQMLASFFKIAPAEILVAHDELDFPPGIVRLK
                     QGGGVAGHNGLKDVSARLGSHDYWRVRIGIGHPGDKRAVTDYVLHKPAQEERAEIDAA
                     VGRALEALPLMLAGDLQGAMLKLHSGEKAETAQRPEKPKADKPEKPAKTGKPEKPGKA
                     EKAETAADAGKTEKTGRLGSFLRKFLPDSDPARKK"
     gene            complement(1741..2496)
                     /locus_tag="AC16CX_13935"
     CDS             complement(1741..2496)
                     /locus_tag="AC16CX_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR3013642.1"
                     /translation="MEVTAYPRAQQGSGASRRLRVAGRVPGVVYGADKPAQNVEFDHN
                     ALLHHLRLEAFHASILDLTLNGEKMRVLLRDYQMHPWKKQVLHVDFQRVDRNRKIRMK
                     VPLHFVNGEISPGVKTGGGVVQHVMNEIDIQCLPDDLPEFVAVDLKDMQLNQTLHLAD
                     LALPKGVEPVAKLKHENPPVVSVHVPKEIVIEEEAPAPVTEIIGEVPAEGEEAAAEAV
                     EGEKAEKAEKKEPVAEKKEPAAEKKEPGKKGKE"
     gene            complement(2646..3578)
                     /locus_tag="AC16CX_13940"
     CDS             complement(2646..3578)
                     /locus_tag="AC16CX_13940"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR3013643.1"
                     /translation="MVFTGNANPRLAEDVVRHLNIHLGRAKVGRFSDGEVMVEILENV
                     RGHDVFVLQSICSPTNDNLMELLVMVDALKRASAGRVTAAIPYMGYARQDRRPSSARV
                     PISAKVVANMMSSVGVDRLLTMDLHSEQIQGFFDIPVDNIYSGPIMLGDVWKLGYSDL
                     VVVSPDVGGVVRARALAKRLEADLAIIDKRRPRPNVATVMNIIGEVEKRTCVIVDDLV
                     DTANTLCEAAGALKERGAARVLAYCTHAVLSGKAVERITGSVLDEVVVTDTIPLPADA
                     KNCRKIRVLTVASLLAETMRRISNEDSVSSLFVE"
     gene            complement(3677..3750)
                     /locus_tag="AC16CX_13945"
     tRNA            complement(3677..3750)
                     /locus_tag="AC16CX_13945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3714..3716),aa:Gln,seq:ttg)
     gene            complement(4014..4859)
                     /gene="ispE"
                     /locus_tag="AC16CX_13950"
     CDS             complement(4014..4859)
                     /gene="ispE"
                     /locus_tag="AC16CX_13950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3013644.1"
                     /translation="MSETASPGYPAPGKLNLMLRVTGRRADGYHLLQTVIRFIDYGDT
                     LTFRLREDGVIARGNEVAGVPAGDDLALRAARLLQQAAGARLGAEISLEKQLPVGGGL
                     GGGSSDAATALLALNHLWRTGLPRERLLALALEIGADVPVFVGGENALAEGIGERLTP
                     LDLPPAWYLVLEPQVAVPTARIFAHPELKRDSKPIKISAFSVEQAGNDLEPVVCREYP
                     EVARHLEWLRRAAPAWVTGSGACVFAAFPTESAARQVWEQAPNDMRGFVTQGLARHPL
                     RDLAR"
     gene            complement(4856..5434)
                     /gene="lolB"
                     /locus_tag="AC16CX_13955"
     CDS             complement(4856..5434)
                     /gene="lolB"
                     /locus_tag="AC16CX_13955"
                     /inference="COORDINATES: protein motif:HMM:NF015512.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HIR3013645.1"
                     /translation="MTWLKSCVAVAAGATLAACAALPLPGERVPRATAFDLVGRVAVN
                     YDGRAFSSNVRWQHLAERDEIWLLTPMGQALAYIVADADGATLTGSDRNQYRAGDVES
                     LTRRALGWELPLERLAWWVRGEIMPGGLIGEVLRDQQGRLVRLGQDGWHITFVHPPAN
                     ERGDLPQRLELVRDAHQIRLVIDGWRDDAGVP"
     gene            complement(5453..>7069)
                     /locus_tag="AC16CX_13960"
     CDS             complement(5453..>7069)
                     /locus_tag="AC16CX_13960"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013646.1"
                     /translation="PEPRKPALPNVELSEELMLKMMLAEVAAQRGQPHIAVQAYLELA
                     RETRDPRVAQRATEVAWNARFTSAALEAAGIWLQADPGSTQARQIIAALLVNQAQLAE
                     ARPHLEKWIAADRDNVGPSFLQLNSLFARHKDKAEVFKLMQSLAKPYPEVPEARLAVA
                     QAAWNAGEQQASLDEARAALKLRPDWEIAALFVAQVLARRSEEEAARYLGEYLRNHPK
                     ARDVQLNYARLLVNLKNYAEARKQFEALVAEYPQNADVGMAVALLAIQATDYDAAETQ
                     LKRVLELNYKDPDVARFYLGQLNEERKRFDEALKWYATVTEGEQYINAQSRYAGILAK
                     QGKLPEARRHLQEATAQNNQQRVQLTQAEAQLLRDASEYQAAFELLGQALEKMPNTPD
                     LLYDHAMAAEKVNRLDVLEGNLKRLIKLRPDHAHAYNALGYTFADRNQRVGEAYALIE
                     TALKLAPEDPFIMDSMGWVLYRMGRNQEGLGYLQRAFKQRPDPEIAAHLGELLWVLGQ
                     RDEARKIWAESLKDHPNNEVLQSTIKRFVP"
BASE COUNT         1070 a         2426 c         2247 g         1326 t
ORIGIN      
        1 agatcgtacg cggcgtgaac aacccggtcg agtcctggtt cttcgagccc gagatcggcc
       61 aggaaagctt tcttgtcttc ctcgcccatg ccggccatct ccgcctcgat ggcggcgcag
      121 atcgccacca ccggcgcgcc ctccttcgct gcgaactccc gtacccgctc aagcagcggg
      181 ttgttcaaga agccatgctc gcccacgttg gcgacgtaga tcgcgggctt ggtggtgagc
      241 aggcacaagg gttgcagaag tttcagttcc tcggcctcga gcccgaggcc gcgcacgggc
      301 ttgccctgat cgaggcgagc gcgtgcccgg gccagcacgc cttcgaacct gatcgcctcc
      361 ttgtctcccg acctggcctt gggagcaacg cggtccagca ccttgtccac cgtcgcgaga
      421 tcggccagcg cgagttcaag ctggacggtc tcgatgtccg cgaccggatc gatcctgccc
      481 gtgacgtgca ccacgttctc gtcttcaaag cagcgcacca gatcgatgat gccgtcggtc
      541 tcgcggatgt gcgcgaggaa ctggttgccc aggccctcgc ctttcgacgc ccccgccacc
      601 agccccgcga tgtccacgaa ctcgacgacg gcaggggtca ccttggcggg ccgggcaatc
      661 tccgcgatcc gcgccaggcg cgggtccggc accggcacca ccccaacgtt gggctcgatc
      721 gtgcagaacg ggtagttctc ggccgggatg gccgcccgcg tgagcgcgtt gaacaacgtg
      781 gacttgccga cgttgggcag gcccacgatg ccgcacctga ggctcatttc ttgcgggccg
      841 ggtcggagtc cggcaggaac ttcctgagaa acgagccgag tctcccggtt ttttcggttt
      901 tgccggcgtc agcggccgtt tccgcttttt cggccttccc cggtttctcc ggtttgcctg
      961 tcttggccgg tttttccggc ttgtccgctt tcggcttctc gggacgttgc gccgtctcgg
     1021 ctttctcccc gctgtgcagt ttcagcatcg cgccctgcag gtccccggcg agcatgagcg
     1081 ggagcgcttc gagcgcacgc ccgacggcgg cgtcgatctc ggcgcgctcc tcctgcgccg
     1141 gcttgtgcag cacgtagtcg gtgacggccc gcttgtcgcc cgggtggccg atgccgatgc
     1201 gcacccgcca gtaatcgtgc gagccgaggc gggccgagac gtccttcagc ccgttatggc
     1261 ccgcaacgcc gccgccttgt ttcaggcgga cgatgcccgg cgggaaatcg agctcgtcgt
     1321 gcgccacgag gatctcggcg ggcgcgatct tgaagaagct tgccagcatt tgcaccggcc
     1381 gcccgctcgc gttcatgtag ttctgcggga gcagcagcca cgccccgctc gcctcgtgcc
     1441 gtccgaccag cgcctggaac ttcgcatcct tgcgcagcac ggtgcccgtt tgcgccgcga
     1501 agcgctcgac gagccagaag cccgcgttgt gccgcgtgcc ctggtggcgg tcccccggat
     1561 tgccgagccc gacgatcagt ttcataggat ccgtgaggcg tgaggggtga ggagtgaggc
     1621 gtcaaaccca aacctgttca ttgcattgcc tccgctcgcc tcacgcctga cgcctcacgc
     1681 cgttgcgcga aaaagcccgc catgccatac ggactggcgg gctttttcgg caggcggagc
     1741 ttattccttc cctttcttgc ccggttcctt cttctcggca gctggctcct tcttctcggc
     1801 gaccggctcc ttcttctcgg ctttttcggc cttttcgccc tccacagcct cggcggcagc
     1861 ctcctcgccc tcggccggga cttcgccgat gatctcggtc accggggcgg gcgcttcttc
     1921 ctcgatcacg atctccttcg gcacgtgcac cgacaccacc ggcgggttct cgtgcttgag
     1981 cttcgccacg ggctcaacgc ccttgggcag cgcgaggtcg gcgaggtgaa gcgtctggtt
     2041 gagctgcatg tccttgaggt ccaccgcgac gaactccggc aggtcgtcgg gcaggcactg
     2101 gatgtcgatc tcgttcatca cgtgctgcac caccccgccg ccggtcttca cgcccgggct
     2161 gatctcgccg ttgacgaagt gcagcggcac tttcatgcgg atcttgcggt tgcggtcgac
     2221 gcgctggaag tccacgtgca gcacctgctt cttccacggg tgcatctggt agtcgcgcag
     2281 cagcacccgc atcttctcgc cgttcagagt caggtcgagt atcgatgcat ggaaagcctc
     2341 gagcctgaga tggtgcagca gcgcattgtg gtcgaactcg acgttctgcg ccggcttgtc
     2401 cgcgccgtag acgacgcccg gcacgcgtcc ggctacgcgc aggcggcggc tcgcacccga
     2461 tccctgttgc gcgcgcggat aggcggttac ttcgatcttc attgcgcttc tcctgtcaat
     2521 tcgggacgtc cgcgaccaga ctgtcccggg gttaaaaatc agtgttgagt attgagtgtt
     2581 gagtgctaag ttgtgaatcg ctcttcaact caaaactcaa cactaaaaac tcatcactca
     2641 gttcgctatt cgacgaagag cgaactgacg gagtcctcgt tgctgatgcg gcgcatggtc
     2701 tccgccagca ggctcgcgac cgtgagcacc ctgatcttcc ggcaattctt cgcatctgcg
     2761 ggcagcggga tggtgtcggt caccaccacc tcgtcgagca ccgagccggt gatgcgttcc
     2821 accgcctttc ccgagagcac ggcgtgcgtg cagtaggcga gcacgcgcgc cgccccgcgc
     2881 tccttcagcg cccctgccgc ctcgcacagc gtgttggcgg tatcgaccag gtcgtccacg
     2941 atcacgcagg tgcgtttctc cacttcgccg atgatgttca tcaccgtcgc gacattcggg
     3001 cgcggccgcc gcttgtcgat gatggcaaga tcggcctcga gccgcttcgc cagcgcacgc
     3061 gcccgcacga caccgccgac gtccggcgac actaccacca ggtcggaata gcccagcttc
     3121 cacacgtcgc cgagcatgat cggtcccgag tagatgttgt ccaccgggat gtcgaaaaac
     3181 ccctggatct gctccgagtg caggtccatg gtgagcagcc ggtccacccc gacgctcgac
     3241 atcatgttgg cgaccacctt ggcggagatc ggcacccggg cggaactcgg gcgccggtcc
     3301 tgccgcgcgt atcccatgta cgggatggcg gcggtcacgc gacccgcgga ggcgcgcttc
     3361 agagcgtcca ccatcaccag cagctccatc aggttgtcgt tggtcggcga gcagatcgac
     3421 tgcagcacga acacgtcgtg cccgcgcacg ttctcgagga tctccaccat cacctcgccg
     3481 tcgctgaaac ggccgacctt ggcgcggccg aggtgaatgt tgagatgccg gacgacgtcc
     3541 tcggcaagcc tggggttggc gttgccggtg aacaccatca agcggtcgaa tcccgcgctt
     3601 tcagctttat gtgcctgcag catctcgatc tcgattcatg tccgggcgac tgctcgccgt
     3661 gcccggcaat attaggctgg ggaggtagga ttcgaaccca cgcatgccgg aatcaaaatc
     3721 cggtgcctta accagcttgg cgactcccca acatatggcg tcaggaaccc cttggccggc
     3781 attcgcgccg cccgcccgaa atcccgggtc cgccaaggat tttgccggat tgcgctccgg
     3841 caaaatcctt tggcaaaacc gccttcaaaa accggcaccg cgaacacctc aagttacgtc
     3901 tttaggtcgc cccacctgtc gccaaaatca gttagggacg tccggtctat agtactgata
     3961 ctgaggcggt tttgctcata gcagcctgtt cacgcgctga gtctggcagg ctgctatcgc
     4021 gccaggtcgc gcagcggatg ccgcgccaaa ccctgcgtga cgaacccccg catgtcgttg
     4081 ggcgcctgtt cccacacctg gcgagccgcg ctttcggtcg ggaatgcggc aaacacgcag
     4141 gccccgctgc cggtcaccca tgccggcgcg gcccgcctga gccactccag gtgtcgcgcc
     4201 acttccggat attcccggca caccacgggc tccagatcgt tgcctgcctg ctcgaccgaa
     4261 aaggcggaga ttttaatggg ttttgagtcc cgtttcaatt caggatgggc gaaaatccgg
     4321 gcggtcggca ccgccacctg gggctcgagc acgagatacc aagccggggg caggtcgagc
     4381 ggcgtcagac gctcgccgat gccctcggcc agcgcgtttt cgccgcccac gaataccggc
     4441 acgtcggcgc cgatctcgag cgcgagtgcg aggagccgct cgcgtggaag gcctgtacgc
     4501 cacaggtggt tcaatgcgag cagcgccgta gcggcatccg agctgccgcc gccgagcccg
     4561 ccacccaccg gcagctgttt ctcgagggag atttcggcgc cgagccgcgc gccggcggcc
     4621 tgctggagca gccgcgccgc ccgtaacgca aggtcgtcgc cggcagggac gccagcgact
     4681 tcattccccc gggcgatcac cccgtcctca cgcaggcgga aggtgagcgt gtcgccgtaa
     4741 tcgatgaagc ggatcacggt ttgcagcagg tgatagccat cggcgcgccg cccggtgacg
     4801 cgcagcatca ggttgagctt gcccggcgcg gggtagcctg gagacgctgt ctcgctcatg
     4861 ggacgccggc gtcgtcgcgc cagccgtcga tcaccagccg gatctggtgc gcatcgcgta
     4921 ccagctccag tcgctgcggc aggtcgccac gctcgttcgc gggaggatgg acaaaggtga
     4981 tgtgccagcc gtcctgcccg aggcggacca gccggccctg ctggtcgcgc aacacctccc
     5041 cgatcaggcc gccgggcatg atctcacccc tcacccacca cgccaggcgc tcgagcggca
     5101 attcccagcc cagggcccgg cgcgtgaggc tctcgacatc gccggcacga tattggttgc
     5161 ggtcggatcc ggtgagggtg gcaccgtccg cgtcggccac gatatacgcg agcgcctggc
     5221 ccatcggcgt caacagccag atttcgtcgc gctccgcgag gtgctgccag cgcacgttgg
     5281 aggagaaggc gcgtccgtcg taattcaccg ccacgcgtcc gacgaggtcg aaagcggtcg
     5341 cgcgcggcac ccgctcgccc ggaaggggca gcgcggcaca ggcggcaagc gtggcgcccg
     5401 ccgccaccgc gacgcacgac ttgagccagg tcatctgagg atcgcttcgc ggctacggta
     5461 cgaagcgctt gatggtgctt tgcaacacct cgttgttggg atggtccttg agcgactcgg
     5521 cccagatttt cctcgcctca tcgcgctggc ccagcaccca cagcagctcg ccgaggtggg
     5581 ccgcgatctc gggatcgggg cgttgcttga acgcgcgctg caggtagccg agaccttcct
     5641 ggttgcggcc catgcggtac agcacccagc ccatgctgtc catgatgaag gggtcctccg
     5701 gggccagctt caacgccgtc tcgatcagcg cataggcctc cccgacgcgc tggttgcggt
     5761 cggcgaacgt gtagccgagc gcgttgtagg cgtgggcatg gtcggggcgg agcttgatca
     5821 accgtttcag gttgccctcg agcacgtcga gacggttcac cttttcggcc gccatcgcgt
     5881 ggtcgtagag caggtccggg gtgttgggca ttttctccag cgcctgcccc agcagctcga
     5941 acgccgcctg atactcactg gcgtcccgca gcagctgcgc ctcggcctgc gtgagctgca
     6001 cgcgctgctg gttgttctgg gcggtcgcct cctgcaggtg cctgcgcgcc tcgggcagct
     6061 tgccttgctt ggcgagtatg ccggcgtagc gcgactgggc gttgatgtac tgctcgccct
     6121 cggtgacggt ggcgtaccac ttgagcgcct cgtcgaagcg cttgcgctcc tcgttgagct
     6181 gcccgaggta gaagcgcgcg acatcggggt ccttgtagtt cagctccagc acgcgcttca
     6241 actgcgtttc ggccgcgtcg tagtcggtcg cctggatcgc gagcagcgcg accgccatgc
     6301 cgacgtccgc gttctgggga tattcggcga ccagcgcttc gaactgcttg cgcgcctcgg
     6361 cataattctt gaggttgacc agcaggcgcg cgtaattgag ctgcacgtcg cgcgctttgg
     6421 ggtggttccg gagatactcg cccaggtagc gggccgcctc ctcttcggag cggcgggcaa
     6481 gcacctgcgc gacgaacagt gcggcgattt cccagtcggg cctcaacttg agcgcagcgc
     6541 gcgcctcatc cagcgacgcc tgctgctccc cggcgttcca ggcggcctgt gccaccgcga
     6601 gccgcgcctc cggcacctcg ggatagggct tcgccagcga ctgcatcagc ttgaacacct
     6661 cggccttgtc cttgtggcgc gcgaacagcg agttcagctg caggaagctc ggaccgacat
     6721 tgtcgcggtc ggcggctatc catttctcca ggtgcggccg ggcttccgcg agctgcgcct
     6781 ggttgaccag caaggcggcg atgatctggc gcgcctgcgt cgagcctgga tcggcctgca
     6841 gccagatgcc ggctgcctcc agcgccgccg aggtgaagcg cgcgttccac gccacctcgg
     6901 tcgcgcgctg cgcgacgcgc gggtcgcggg tttcgcgcgc gagctcgaga taggcctgca
     6961 ccgcgatgtg cggctgcccg cgctgcgccg cgacctccgc cagcatcatc ttgagcatca
     7021 gctcctcgga gagctcgacg ttgggcaacg cgggcttgcg cggctccgg
//
LOCUS       DBNGWZ010000455         3995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1821931, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000455 DBNGWZ010000000
VERSION     DBNGWZ010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1821931"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="AC16CX_13965"
     CDS             <1..1862
                     /locus_tag="AC16CX_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HIR3013647.1"
                     /translation="DLIIRWVRGEDNFEDENSNASKADIRASVHGDVLHSRPAVVNYN
                     RFANSDDDVFIFYGANDGIFHAVKGGYTVPAGDVSGLTPGREAWGFVPQEFFGQFKRM
                     RNNAPTISSAFKKPYYMDGPIGIYTSDACCLADSSPGPNNKLGDSGDTVNLYIANRRG
                     GRFINALDVNNPTNPRFLWKISNDTAGFGELGETWSQPTVITNLGGLTVPVLVFGAGY
                     DPAVEDLDPSVVLSVNTLTGAVTTTTVGTVNRTMGRGIYVVNALTGALIWRALGASDG
                     TNIDKTTIVPDMIYAIPSDVTVIKNESGGTTNRAYVGDTGGNVWRIDFGSPDGTTLPT
                     CDGVTNVRPNCTLVTKLASVADQSTASGRRKFLFPPDVVGETGFDAVLIGSGDREHPF
                     DTSVTNRFYMFKDKGPDTGPITGTTTCDPTITEAPILDLADACPPAPDPGATPPQTGD
                     IPGLSDLTSNCIQDAAACAAAGQDQAVVKAKLGTDRGWFITLLPGEKVVGNAVSISGT
                     TFFNTNQPSSTAGGGTCGSNLGVARQYQVATADATATSDLNAVGGLTGADRSLIHAGG
                     GYLPSPVHVVVMLGGKPVEAVISGIQVSTPAGATLSARLRKYWYKEVDLRPGP"
     gene            1981..2619
                     /locus_tag="AC16CX_13970"
     CDS             1981..2619
                     /locus_tag="AC16CX_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR3013648.1"
                     /translation="MVSAVMWSPDSIPRSTNRLIVGLALSVLAHLALIAGVRPMVAAY
                     VPFVPLEVEIRHLATEPDAPFSSPAPSDYSADAAMAPAEARKIEPTPPDKAAVPDPRF
                     DPRLPLEHYYTARELDVRAEPLNDVALVYPQLAYQQRIRGKVMLRILINERGGIDQVS
                     VLESEPRDVFEEAALTATRALEFSPALKNGRRVKSQKTIEVTFDPYESINIP"
     gene            2742..2981
                     /locus_tag="AC16CX_13975"
     CDS             2742..2981
                     /locus_tag="AC16CX_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013649.1"
                     /translation="MNKLIAGLCCLMLAVSPLALAQGADAGKKSAPPSAKEQAEQKKA
                     IHAQASRCANDAQRNKLKQGSKEFVNFMTQCLSQK"
     gene            complement(3078..>3995)
                     /gene="ispH"
                     /locus_tag="AC16CX_13980"
                     /gene_synonym="lytB"
     CDS             complement(3078..>3995)
                     /gene="ispH"
                     /locus_tag="AC16CX_13980"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3013650.1"
                     /translation="LLANPRGFCAGVDRAIEIVERALALHGAPIYVRHEVVHNKFVVA
                     DLGAKGAVFVEDLSEVPGGATVIFSAHGVSQAVRREAEARRLKVFDATCPLVTKVHVE
                     VAKMREHGREIVMIGHRGHPEVEGTMGQSAGGMYLVEKPEDVARLRVKDENNLAFVTQ
                     TTLSVDDAQQTISALKARFPAIVGPKKDDICYATQNRQDAVKALARRCDVVIVVGSPN
                     SSNSNRLREVAENQGIAAYMVDNASELRPEWIAGKGVVGITAGASAPEVLVQQVIERL
                     RALGAENVSQLEGIEEGVTFPLPKGLVAV"
BASE COUNT          714 a         1360 c         1280 g          641 t
ORIGIN      
        1 gcgatctgat catccgctgg gtgcgcgggg aggacaactt cgaggacgag aactcgaacg
       61 cctcgaaggc cgacatccgc gcctcggtgc acggcgacgt gctgcattcg cgcccggcgg
      121 tggtcaatta caaccgcttc gccaattcgg atgacgatgt cttcatcttc tacggcgcga
      181 acgacggcat cttccacgcc gtcaagggcg gctacaccgt gcccgccggc gacgtctcgg
      241 ggttgacgcc aggccgggaa gcgtggggct tcgtcccgca ggagttcttc ggccagttca
      301 agcgcatgcg caacaacgcg ccgacgatct cgagcgcgtt caagaagccg tactacatgg
      361 acgggccgat cggcatctac acctcggatg cgtgctgcct ggcggacagc agtcccgggc
      421 ctaacaacaa gctcggagac agcggcgaca ccgtgaacct ctacatcgcg aaccggcgcg
      481 gcgggcgctt catcaacgcg ctcgacgtga acaacccgac caatcccagg ttcctgtgga
      541 agatcagcaa cgacacggcg ggcttcggtg agctcgggga gacctggtcg cagccgaccg
      601 tcatcaccaa tctcggcggg ctgaccgttc cggtcctggt gttcggcgcc ggttacgacc
      661 cggcggtgga agatctggac ccctccgtgg tactcagcgt caacaccctc accggcgcgg
      721 tcacgaccac cacggtcggc accgtcaatc gcaccatggg ccgcggcatc tacgtcgtca
      781 atgcgctcac cggcgcgctg atctggcgcg cgctcggcgc ctcggacggg acgaacatcg
      841 acaaaacaac gatcgtgccc gacatgatct acgcgatacc gtcggatgtc acggtgatca
      901 agaacgagag cggcggcacg accaaccggg cctatgtcgg cgataccggc ggcaacgtct
      961 ggcgcatcga cttcggaagc cccgatggga cgactctgcc cacttgtgac ggggttacga
     1021 acgtacggcc caattgcacg ctcgtcacca agctggcgtc ggtcgccgac cagtccaccg
     1081 cctcgggcag gcgcaagttc ctgttcccgc ccgacgtcgt cggggaaacc gggttcgacg
     1141 cggtgctgat cggctccggc gatcgcgagc atccgttcga cacctcggtg acgaaccgct
     1201 tctacatgtt caaggacaag ggccctgaca cgggacccat taccggcact acgacgtgcg
     1261 atccgacgat cacggaagcg ccgatcctgg acctggccga tgcgtgcccc cccgcccctg
     1321 accccggcgc aacccccccg cagaccggcg atattccggg attgtccgac ctcacctcca
     1381 actgcatcca ggatgccgcg gcgtgcgcgg cggcggggca ggatcaagcg gtcgtcaagg
     1441 ccaagctcgg caccgaccgg ggctggttca tcacgctcct gccgggcgag aaagtggtcg
     1501 gcaacgcggt ctcgatcagc ggcaccacct tcttcaacac caaccagccc tcgtccacgg
     1561 cgggcggcgg cacctgcggc agcaacctcg gcgtggcgcg gcagtaccag gtcgccacgg
     1621 cggacgccac cgcgaccagc gacctgaacg cggtcggcgg attgaccggc gcggaccgct
     1681 cgctgatcca cgcgggcggc ggctacctgc cctccccggt gcacgtcgtc gtcatgctcg
     1741 gcggcaagcc ggtggaggcg gtcatttcgg gcatccaggt gagcacgccg gccggcgcca
     1801 cgctgtcggc gcggctaagg aagtactggt acaaggaagt ggacctcaga cccgggccat
     1861 aaacccgtgc cagccgccgc ttacgttgac gcggtaacag ggcagcgcgg ctgccctgtc
     1921 ttccatttgg cggcggaaag tggcaggggc ttcgcgaagc tcgaagcacc gctcccttct
     1981 atggtttcag cagtcatgtg gtcgccggac tcgattccac ggtccaccaa ccggttgatc
     2041 gtcggattgg ctctctcggt gctggcacat ctcgcgctga tcgcgggcgt gcggccgatg
     2101 gtggccgctt acgtcccgtt cgtgccgctg gaggtggaaa tccggcacct cgcgaccgag
     2161 ccggacgccc cgttctcaag cccggccccg tccgattact cggcggatgc cgccatggcg
     2221 ccggcggagg ccaggaaaat cgagcccacg ccgccggaca aggccgccgt gcccgatccc
     2281 cgtttcgacc cgaggctgcc gctcgagcac tactacacgg cgcgcgagct ggacgtgcgc
     2341 gcggagccgc tgaacgacgt cgcgctcgtg tacccgcagc tcgcttacca gcagcgcatc
     2401 aggggcaagg tcatgctgag gatcctcatc aacgagcgcg gcggcataga ccaggtgtcg
     2461 gtgctggagt cggagccgcg cgacgtgttc gaggaggccg cgctcaccgc gacgcgggcg
     2521 ctggagttct cgcccgcgtt gaagaacggg cgcagggtca agagccagaa aaccatcgaa
     2581 gtgacgttcg acccttacga gagcatcaac attccctgag gaaagcgaca aagtcagcgt
     2641 gacggagact ggcgcgttat cagtagaagc cgagcctgct gacgccaggt gtccttccag
     2701 agagatggcg tgttggagat taacgagatg aggagccgag aatgaacaag ctgatcgcag
     2761 ggttgtgttg cctgatgctg gcggtgtcgc cgctcgcgct ggcgcagggc gcggatgccg
     2821 ggaaaaagtc cgcacccccg tccgcgaagg aacaagcgga acagaagaag gccattcacg
     2881 cgcaggcgtc acggtgcgcc aacgacgcgc agagaaacaa gctgaagcag ggcagcaagg
     2941 aattcgtcaa cttcatgacg cagtgcctga gccaaaaata acggtcgcga cgcaaggatt
     3001 tcccctgccc cgcgcgaagc atggcgcggt gatccgcact ccccgcttgc cttatccctg
     3061 actcggggcc gcagaatcta aaccgcaacc agccccttcg gcagggggaa ggtgactccc
     3121 tcttctattc cctcgagctg gctgacgttc tctgcgccca gcgcccgcag gcgttcgatc
     3181 acctgctgaa ccagcacttc aggggctgaa gcgccggcgg tgattccgac gacgcccttg
     3241 ccggcgatcc attccggcct gagctcggag gcattgtcca ccatgtaggc ggcgatgccc
     3301 tggttctccg ccacctcgcg cagccggttc gagttcgagc tgttgggcga gcccaccacg
     3361 atcacgacgt cgcaccgcct ggcgagcgcc ttgaccgcgt cctggcggtt ctgcgtcgcg
     3421 tagcagatat cgtccttctt cggcccgacg atggcgggaa agcgcgcttt cagcgcactg
     3481 atcgtctgct gcgcgtcgtc caccgacagc gtggtctggg tcacgaaggc gagattgttc
     3541 tcgtccttca ccctgagccg cgcgacgtcc tccggcttct cgaccaggta catgccgccc
     3601 gcgctctggc ccatggtgcc ctcgacctcg ggatggccgc gatggccgat catgacgatc
     3661 tcgcggccgt gctcgcgcat cttggcgact tcgacgtgca ccttggtcac cagcgggcag
     3721 gtcgcgtcga acaccttcag ccggcgcgcc tcggcctcgc gccgcaccgc ctgcgacacg
     3781 ccgtgggcgc tgaagatgac ggtcgcgccc ccgggcactt cgctcaggtc ctcgacgaat
     3841 accgcgccct tggccccgag gtcggcgacc acgaatttgt tgtgcaccac ctcgtggcgc
     3901 acgtagatcg gcgcaccgtg caacgcaagc gcgcgctcga cgatttcgat cgcgcggtcg
     3961 acgccggcgc agaatccgcg gggattggcg agcag
//
LOCUS       DBNGWZ010000456         2544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3459968, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000456 DBNGWZ010000000
VERSION     DBNGWZ010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3459968"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="AC16CX_13985"
     CDS             complement(<1..944)
                     /locus_tag="AC16CX_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3013651.1"
                     /translation="MTQDKQSARRPCSRREFLALGAGLAASALGMAPVRAQIQTAKPL
                     LARKIPLTGEPLPVIGLGTAIVFDIGDDAVRRAERAAVIRALLEGGARLIDTAPSYGT
                     AESVVGDLLAEMKVRGRVLIATKVRVAGRDRAVAEMRQSQRQLRTEKIDLMQIHNVGF
                     ADRGEVGAQLALLREWKDRGVFRYIGVTHSQDQASANARLVELMQREKLDFMQVNYSM
                     AERSVEQKLLAAAADTGTAVLVNLPFARGRLFRAVSGRRVPEWAREFDAPTWGQFFLK
                     YILASEAVNAVLPGTDKPEYMLDNLAAARTRLPDAAQR"
     gene            complement(946..2286)
                     /locus_tag="AC16CX_13990"
     CDS             complement(946..2286)
                     /locus_tag="AC16CX_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP/NDP exchange transporter"
                     /protein_id="HIR3013652.1"
                     /translation="MRESVLHRWLASVVAVRPGEARAMCWSFAYFFCLLAGYYVLRPL
                     RDEMGIAGGVRNLQWLFTATFFVMLAAVPVFGAVVAHLPRRRFIPLIYHFFVANIAIF
                     WVLLTLDVGTVHVARVFFVWISVFNLFAVSVFWSFMADLYASEQGKRLFGFIAAGGSA
                     GALLGPALTVGLAVPLGPVNLLIVAALLLEAAVFCASRVENAASQLKSAADTPAAAVQ
                     AAGSDAAGLGGGWLDGIAMVLRSPYLAGIALWVALLSLAGTFLYFQQANIVAAASDDP
                     AVRTRIFATIDLAVGILTLLVQCFATGRLITRFGAGPAAAFLPLAFSLGFLALWLTPM
                     LWVVIAFQAVQRAANFAISNPAREVLFTVLDREEKYKAKNVIDIVVFRGADAASGWLF
                     AALRGAGLELGAISLATVPVTAGWLALALALGRVHERRTRGSALTVPPPTTRGA"
     gene            complement(2293..>2544)
                     /locus_tag="AC16CX_13995"
     CDS             complement(2293..>2544)
                     /locus_tag="AC16CX_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR3013653.1"
                     /translation="AFNSVGYSRLQSEAHRSGERVAIPLAADDAQALQTAARLVRDAG
                     FEPVVVGPLARAKEFDAGTPVFGKALTAAELRSGLGINP"
BASE COUNT          453 a          851 c          883 g          357 t
ORIGIN      
        1 ctgcgttgcg ccgcgtcggg caggcgggtg cgcgcggcgg cgaggttgtc cagcatgtac
       61 tcgggcttgt cggtcccggg caggacggca ttcaccgcct cgctggcgag gatgtatttc
      121 aggaagaact ggccccaagt cggcgcatcg aattccctcg cccattcagg cacccgcctg
      181 ccgctcacgg cgcggaacag ccggccgcgg gcgaacggca ggttgaccag cactgcggtg
      241 ccggtatcgg cggccgccgc gagcagtttt tgctcgacgc tgcgctcggc catcgagtag
      301 ttaacctgca tgaaatcgag cttttcgcgc tgcatgagct cgaccaggcg cgcgttggcg
      361 cttgcctggt cctgggaatg ggtcaccccg atgtagcgga aaacgccgcg gtctttccat
      421 tcgcgtaaca gcgccagctg cgcgccgacc tcgccgcggt cggcgaatcc gacgttgtgt
      481 atttgcatca agtcgatttt ttcagtgcgc agctggcgct gcgattgccg catctcggcg
      541 accgcgcggt cgcgcccggc cacgcgcacc ttggtcgcga tcaggacccg tccgcgcact
      601 ttcatttcgg caagcaagtc cccgaccacg gactccgccg tgccgtagga cggcgcggtg
      661 tcgatcaggc gcgcgccgcc ctcgagcagg gccctgatga ccgcagcgcg ctcggcgcgc
      721 cggaccgcat cgtcgccgat gtcgaagacg atcgcggtgc ccaggccgat taccggcagc
      781 ggctcgccgg tgagaggaat cttccgcgcg agcaacggtt tagcggtctg gatctgggcg
      841 cggactggcg ccatgccgag cgcgctggcg gcaagcccgg cgccgagcgc caggaactcg
      901 cggcgcgagc aaggacggcg ggcggattgc ttgtcttgtg tcacgtcagg ctcctcgcgt
      961 ggtgggaggt ggaacggtca atgcggaacc gcgcgtgcgg cgctcgtgca ctcgtccgag
     1021 cgccagtgcg agagcgagcc agccggcggt gaccggcacg gttgccagag aaattgcgcc
     1081 cagctccagg ccggcgccgc gcagcgccgc gaacagccat ccgctcgccg catcggcgcc
     1141 gcggaagacg acgatgtcga tcacgttctt ggccttgtac ttttcctcgc ggtccagcac
     1201 cgtgaacagg acttcgcgcg ccgggttcga gatcgcgaag ttggcggcgc gctggacggc
     1261 ctggaaggcg atcacgaccc acagcatggg cgtgagccag agcgcgagga aacccaggga
     1321 gaatgcgagt gggaggaacg cggccgccgg gccggcgccg aagcgcgtga tcagccgccc
     1381 ggttgcgaag cactgcacca ggagggtcag tatcccgacc gccaggtcga tcgtcgcaaa
     1441 gatgcgcgtg cgcaccgccg ggtcgtcgga ggcggcggca acgatgttcg cctgctggaa
     1501 ataaaggaaa gtgcccgcga gggacagcag cgccacccac aacgcaatgc cggcgaggta
     1561 gggcgagcgc agcaccatgg cgatgccgtc cagccatccg ccccctaatc ccgcggcatc
     1621 ggacccggca gcctgcacgg cggctgccgg tgtatcggct gcggacttga gttgcgaagc
     1681 ggcgttctcc acgcgcgatg cgcaaaacac ggccgcctcg agcagcaatg ccgcgacgat
     1741 caggaggttg accgggccga ggggcacggc aagcccgacc gtcagcgcgg gtcccagcag
     1801 cgcgcccgcg gatccgccgg ccgcgatgaa gccgaacagc cgcttgccct gctcggacgc
     1861 gtagaggtcg gccatgaacg accagaacac cgagaccgcg aacaggttga acacgctgat
     1921 ccacacgaaa aacacccggg cgacgtggac cgtgccgacg tcgagcgtca gcagcaccca
     1981 gaagatcgcg atattggcga cgaagaaatg gtagatgagc gggatgaagc gccggcgcgg
     2041 caggtgggcg accaccgcgc cgaacaccgg caccgcggcg agcatgacaa aaaacgtcgc
     2101 cgtgaacagc cattgcaggt tgcgcacccc tcccgcgatg cccatctcgt cgcgcagcgg
     2161 ccgtaacacg tagtagccgg ctagcaggca gaaaaaatag gcaaacgacc agcacatcgc
     2221 gcgcgcttcg cccggccgca ccgcgaccac gctggcaagc cagcggtgaa gcacgctttc
     2281 gcgcattaac gcctacgggt tgatgccgag gccgctgcgc agttcggccg cggtcagcgc
     2341 cttgccgaag accggcgtgc cggcgtcgaa ttccttcgcg cgcgcgagcg gtccgaccac
     2401 gacgggctca aaaccggcgt cccgcacgag gcgcgccgcg gtctgcagcg cctgcgcatc
     2461 gtcggcggcg agtggaatag cgacccgctc gccggagcgg tgcgcttcgc tttgcaggcg
     2521 cgagtagccg acggaattga aggc
//
LOCUS       DBNGWZ010000457         4565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1445428, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000457 DBNGWZ010000000
VERSION     DBNGWZ010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1445428"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC16CX_14000"
     CDS             <1..675
                     /locus_tag="AC16CX_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3013654.1"
                     /translation="SSGFGVHYRFLEYHDMKEVIFNAMRAEKEGYDAFLIGNSSDAGI
                     REAREVANIPVLGLSETSLHIACMMGATFGLLTVSEKWTPRNIENVRRAGLESRLVGC
                     VPLNTSPLELKQAMAEKARRDKVMSDVMAGARSLIERGAEVIIPAGGDIIVFLAEDGI
                     FEIDGAPILNGIFELVKMGEVAAKLRAISGGRFKSKRLGYAAPSGDFLRRIRDFYGPD
                     VYPGAQ"
     gene            complement(817..1779)
                     /locus_tag="AC16CX_14005"
     CDS             complement(817..1779)
                     /locus_tag="AC16CX_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013655.1"
                     /translation="MWRALPIAFSFMLFPLLAGAQDYPSRPVRIIVPTPAGSATDMSM
                     RLVAPGLAGLLGQQFVIDNRAGAGGRIGTEVAAQAAADGYTLVLGTPGTLTIMQYVQK
                     NVPYQTLRDFAPIGLISQGPYLLVAHPSVPVRSVKDLLALAKAQPGKLNYGSAGTGAT
                     NHLAMELFKSMSRANIIHVPYKGGALATTAILSGEIDLTMLSIGPLLPHVRAGKLRAL
                     GITSAKRFALLPDVPTIGESGVPGYEAITWFGLLAPAGTPSAVLARLGDTLQKVLRAP
                     EMREKFQQTGAEPGERYGDEFAELIRRETEVYGKLVKQSGMQAN"
     gene            complement(1994..2791)
                     /locus_tag="AC16CX_14010"
     CDS             complement(1994..2791)
                     /locus_tag="AC16CX_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3013656.1"
                     /translation="MKLWYQSLARQAEAGTYLAVLEKIIGSAADPGTSVHVRAITQSA
                     GIGQHYRFLEYQDTREIIYNAIEAERGGYDAFLIGNISDAGLREVREMVNVPVLGMCE
                     TCLHLACMMGASFGLVAISERWFPKLMENVARCGLDRRLAGIEAMRTSPVDLKKGFVD
                     TGHRHDLIAQFTNATQKLLARGAEVIIPAGGELVALLSQTGTYEIERAPILNGIVELT
                     KMGELAAKLKALTGRFTSKQLHYAPPSGDYLERIRSFYGPEIYPGAE"
     gene            complement(2788..4248)
                     /locus_tag="AC16CX_14015"
     CDS             complement(2788..4248)
                     /locus_tag="AC16CX_14015"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HIR3013657.1"
                     /translation="MREVRIIDTTLRDAPQCLWATRMTAAMMMPVAEMMDRAGFGRID
                     LAAAIQFDVCVRYLKENPWEKFRLLRERIVNTPLQATVRGRSLVSFDILPDDIIGLWV
                     ERLVANGFRVVCAMDGLNDVDNLAVSLKLAKALGVTTEASLAYSLSPVHTDEHYAAAA
                     AEVIGKIGVDIVKIRDAGGLLTPDRVRTLVPALKRVLGKTRLALHGHCLTGLAPLVYL
                     EAIKLGVDEVATSVAPLANGPAQPATQTFARNARLLGFETGVDLELVDRVGEHFRRVA
                     EQEGLPLGVPVEYDEFHFAHQVPGGMLTNLKFQLAQAGLSERFDAVLEECARVREELG
                     WPIMITPFSQLVGTQAVLNVVQGERYRVVPDEVKKYALGHYGRLLAPVKPDVLDRIVE
                     NGSSAIRQTAPAPEPAVPRLRKQCPGKSDDERLLRFMFAGNQVDEMLAAGPLRTEYAF
                     KNQAQRLLETALKTKKGRYISIAEGNVKLEAVRRPK"
     gene            complement(4245..>4565)
                     /locus_tag="AC16CX_14020"
     CDS             complement(4245..>4565)
                     /locus_tag="AC16CX_14020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014804.7"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3013658.1"
                     /translation="PGAIVEWQQPDAAGVRVDTHCYPGYAIPPFYDSMIGKLIVRGAD
                     RREAIEKTLSALGRFVIGGVHTTLPFSRAIVSDDDFRRSAITTDWLERVILPRYLNDS
                     GAQR"
BASE COUNT          788 a         1457 c         1467 g          853 t
ORIGIN      
        1 tcgtccggct tcggcgtgca ttatcgcttt ctcgaatacc acgacatgaa ggaagtcatt
       61 ttcaacgcga tgcgcgccga gaaggaggga tacgacgcgt tcctcatcgg caattcgagc
      121 gatgcaggca tacgggaagc ccgcgaggtc gcgaacatcc cggtgctcgg actgtcggaa
      181 accagcctcc atatcgcatg catgatgggg gcgaccttcg ggctcctgac ggtgagcgaa
      241 aaatggacgc cgcgcaacat cgagaacgtc cgccgcgcgg ggctggagag ccgcctcgtc
      301 ggatgcgtgc cgttgaacac ttcgccgctg gagctcaagc aagccatggc ggagaaggcg
      361 cggcgggaca aggtgatgtc ggatgtcatg gcgggagcca ggagcctcat cgagcgcggg
      421 gcggaagtga tcattcccgc cggcggcgac atcatcgtgt ttcttgccga agacggcatt
      481 ttcgagattg acggcgcgcc gatcctgaac ggcatattcg agctcgtgaa gatgggggaa
      541 gtcgcggcca agttgagggc gatttcgggc ggacgattca agagcaagcg cctcggttac
      601 gccgcgccga gcggggactt tctcaggcgc atccgggatt tttacggacc cgacgtctac
      661 ccgggtgcgc agtgaaatga aaccggcgtc gcgagaccgc gccggcctgc ggctggatcc
      721 aggggctggt gcaatggctg cttggtctgc gtggcgcgca gccgttggcg ctcgcggccg
      781 atcccggcga tccgggggcc tgcagcgcgg atgcgatcag ttggcctgca ttccggattg
      841 cttgacgagc tttccgtaga cctcggtctc ccggcggatc agctccgcaa actcgtcccc
      901 gtacctttcc cccggttccg ctccggtctg ctggaatttc tcccgcattt ccggcgcgcg
      961 gaggactttc tgcaaggtat cacccaggcg agcgagaacg gcactgggcg tccccgcagg
     1021 cgcgagcagc ccgaaccagg taatggcttc atagccgggc acgcctgatt cgccgatggt
     1081 gggcacgtcg ggcagcagcg caaagcgctt cgcgctcgtg atgccgagcg cgcgcagctt
     1141 ccccgcccgg acatgcggca gcaagggccc gatggaaagc atggtcaggt caatttcgcc
     1201 gctcaggata gcggtggtcg ccagcgcgcc gcccttgtaa ggcacgtgaa taatgttggc
     1261 gcgcgacatg ctcttgaaca gctccatcgc caggtgattg gtggcgccgg tgccggccga
     1321 gccgtagttg agcttgccgg gctgtgcctt tgcgagcgcg aggaggtcct tcacgctacg
     1381 caccggcacc gacgggtgcg caaccagcag ataagggcct tgggagatca gtccgatcgg
     1441 ggcgaaatcg cgcagcgtct ggtagggaac gtttttttgc acgtactgca tgatggtgag
     1501 cgtgcctggc gtgcccagca cgagcgtata tccgtcggct gcagcctggg ccgccacttc
     1561 ggtgccgatc cggccgccag cgcccgcgcg gttgtcgatc acgaactgct ggccgaggag
     1621 gccggccaat ccgggggcga ccagccgcat cgacatatcg gttgccgacc cggcgggagt
     1681 cggaacgatg atgcggaccg gccggctcgg atagtcctgg gctcccgcga gcagcggaaa
     1741 cagcatgaag gaaaatgcga tgggcagcgc gcgccacatg gtgaatgacc tcccctgatt
     1801 tatttaactc gacccgcggc agcgaactca atagcccttt cggtagcgat ggcgtactga
     1861 atgtacgcgc gcacgcgcgg atcgtgaagt tcaatcaata gtctatatcc gcggcgttgc
     1921 tcgtgccatt tccagcccga agccttttcg ccggcgcaca cgccggctgg acgcgggttg
     1981 ctcaccgtgg cggtcactcg gcgccagggt agatctcggg gccgtagaag ctcctgatgc
     2041 gctcgagata atcgccagag ggcggcgcat agtgcagttg cttgctggtg aagcgccccg
     2101 tcagagcctt cagcttggcc gccagttctc ccatcttggt caattcaacg atgccgttga
     2161 ggatcggcgc gcgctcgatt tcataggtgc cggtctgcga gagcagcgcg accagctcgc
     2221 cgccggccgg aatgatcacc tcggcgccgc gggcgagcag tttctgcgtc gcgttcgtga
     2281 actgggcaat gaggtcatgg cggtgcccgg tgtcgacgaa gcctttcttc aggtctaccg
     2341 gtgaggtgcg cattgcctcg atcccggcca ggcgccggtc caggccacag cgggcgacgt
     2401 tttccatcag cttcgggaac cagcgctcgc tgatcgccac gagcccgaag cttgcgccca
     2461 tcatgcacgc cagatggagg caggtctcgc acatgcccaa taccgggacg ttgaccatct
     2521 cccgcacctc ccgcaggccc gcgtcgctga tgttgccgat gaggaacgca tcgtagcctc
     2581 cacgttccgc ctcgatcgcg ttgtagatga tttcccgggt gtcctgatat tcgagaaacc
     2641 ggtaatgctg gccgattccg gcggattgcg tgatcgcgcg gacgtgcacg ctcgttccgg
     2701 ggtcggcggc cgagccgatg atcttctcca gcacggccag gtacgttcct gcttccgcct
     2761 gtcgggccag gctctgatac caaagcttca tttcgggcgg cgaaccgcct ccagcttcac
     2821 gttgccttcg gcgatactga tgtagcgccc tttcttcgtc ttcagcgcgg tttccaacag
     2881 cctctgcgcc tggttcttga acgcgtactc ggtgcgcagg gggccggcgg caagcatctc
     2941 gtcgacctga ttcccggcga acatgaatcg cagcaggcgc tcgtcgtcgc ttttccccgg
     3001 gcattgcttg cgcaatcgcg gtaccgccgg ctcgggcgca ggcgcagtct gcctgatcgc
     3061 gctcgacccg ttttcgacga ttctgtcgag cacatccggc tttaccggag ccaggagcct
     3121 gccgtaatgc cccaaggcgt atttcttgac ttcgtccgga accacccggt agcgctcccc
     3181 ctgcacgacg ttgaggaccg cctgggtgcc gaccagctgg gagaagggcg tgatcatgat
     3241 cggccaaccc aattcctccc ggacgcgcgc gcattcttcc agcaccgcat caaagcgttc
     3301 gctcaacccg gcctgggcca gctggaactt gaggttggtg agcatgccgc ctggaacctg
     3361 atgcgcgaag tggaactcgt cgtattcgac cggcaccccg agcggcaggc cttcctgctc
     3421 ggcgacccgc cggaagtgct ccccgacgcg atcgacgagc tcgagatcga caccggtttc
     3481 gaaaccgagc aggcgggcgt tgcgcgcgaa cgtctgcgtg gccggttggg caggaccgtt
     3541 cgcaaggggc gcaaccgatg tcgccacctc atcgacgccg agcttgatgg cttccagata
     3601 cacgagcggc gcgagcccgg tgagacagtg gccgtgcagc gccagtctgg ttttcccgag
     3661 gacgcgcttg agcgcgggaa cgagggttct gacgcggtcc ggcgtgagca ggccgcccgc
     3721 atcgcgaatc ttgacgatat cgaccccgat cttgccgatg acttccgcag cggcggcggc
     3781 gtagtgctcg tcggtgtgga cggggctcag ggaatatgcc agcgacgcct ccgtcgtcac
     3841 gcccagcgcc ttggccagct tgaggctgac cgccagattg tccacgtcgt tcaacccgtc
     3901 catcgcgcac accacgcgaa agccgttggc gaccagccgc tcgacccaca gcccgatgat
     3961 gtcgtcgggc aggatgtcga agctcacgag actgcggccg cgcacggtgg cctggagcgg
     4021 cgtattcacg attctttccc gcagcagcct gaacttctcc cacggattct ccttgagata
     4081 ccgcacgcag acatcgaact ggatcgccgc cgcgaggtcg atgcgcccga accccgcccg
     4141 gtccatcatc tccgccaccg gcatcatcat ggcggcggtc atcctcgtcg cccacaggca
     4201 ctgtggagca tcacgcaggg tggtatcgat tattcgtact tccctcatcg ttgcgccccg
     4261 gaatcattca aataccgcgg aagaataacg cgttcgagcc aatccgtcgt tatcgcggat
     4321 cgcctgaaat cgtcgtcgga gacgatcgcc cggctgaagg gcagtgtcgt gtgcacgccg
     4381 ccgataacga agcggcccag cgcagacaac gttttttcga tcgcttccct cctgtctgcg
     4441 ccgcgcacga tcagtttgcc gatcatcgag tcgtagaacg gcgggatggc gtatcccgga
     4501 tagcagtggg tgtccacgcg taccccggct gcgtcgggct gttgccactc gacgatcgcc
     4561 cccgg
//
LOCUS       DBNGWZ010000458         3546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2201396, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000458 DBNGWZ010000000
VERSION     DBNGWZ010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2201396"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="AC16CX_14025"
     CDS             complement(<1..809)
                     /locus_tag="AC16CX_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3013659.1"
                     /translation="MQPLAGIRVLDFSTLLPGPLATLLLAEAGAEVIKIERPGAGDEM
                     RSYEPKFGADSVNFALLNRGKRSIAVDLKQPAAVGRLMPLVKRSDIIVEQFRPGVMDR
                     LGLGYEAVSRVNPRIIYCTITGYGQSGPRAMTAAHDLNYAAEAGMLALAAGADGAPVP
                     PAALVADIGGGSYPAVINILLALRERERSGRGCKLDIAMADNLFTFMYWAMGDGLAAR
                     RWPQPGKALVTGGSPRYRVYRTRDDRFIAAAPLEERFWQNFCDAIGLAPGDR"
     gene            complement(845..1516)
                     /locus_tag="AC16CX_14030"
     CDS             complement(845..1516)
                     /locus_tag="AC16CX_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3013660.1"
                     /translation="MKHQIIRAGVSRSFPDDHPFLASATDIAEVVVIVGANAGDAFRK
                     LGKTDRRAAVLIELGTECLGTHTGEDRGLEGSNVLGFARFRLGGAGPSNLVELVRQPR
                     TPESAVTAARQVFESAGLQVAVCNDFAGRIVDRLVRPYYNAALRRLDEGLASAADLDT
                     TLKLGLGYPEGPIELLERTGLAQHFDVTQALFEAFGEAAYAPARRARVAKQRREPQIS
                     ADERR"
     gene            complement(1606..2487)
                     /locus_tag="AC16CX_14035"
     CDS             complement(1606..2487)
                     /locus_tag="AC16CX_14035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3013661.1"
                     /translation="MTGDGIRTVGVAGSGTMGAGIAIVTARAGFRTVVYDTRAEALAR
                     ARKQTEGFFAKSVERKKLTQEQVSKILSDLSGTTDIAALAACDIVIEAVFEDLEVKHG
                     LLAQLDKVCPPHAIFASNTSTLSITEIAAGSGRDDRFVGMHFCLPAQLMKLVEMSPGL
                     KTSEATLQAAWDFCKALGQTPVKTQDSPGFILNYFLIPFNNDAIRLVEAGAAAPADID
                     RAVKAGLGYPMGPLELLDLVGLDTHLLVAEALYASTRDPRAAVPALVKRMIAAGKLGR
                     KTGEGFYKYQGSSMFGG"
     gene            complement(2512..3390)
                     /locus_tag="AC16CX_14040"
     CDS             complement(2512..3390)
                     /locus_tag="AC16CX_14040"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013662.1"
                     /translation="MRLVVGFPPGGAADFVSRALQEPLSRALGQPVVVENRPGGSSTI
                     GLDLAAKSPPDGHTWGTPNITFGANPSLMKKMPFNSERDLIPVSQVTVVTMVLSVHPS
                     VPARSVKQLIALAKAHPGALNFGSAGNASANHLATAKFMHMSGTNLTHVPYKGGGPAV
                     ISLVSGETSMLFATIPSAIQHIQGGRLRGLAVSRAQRNPALPDVPTVAEAGVPGYEAI
                     EWNGVMVPAGTPPAVVRRIYQALAKVLAATAMKERIVGVGAEVVGSSPEEFTAFIKSE
                     FSAWARVIKEVGITVE"
BASE COUNT          573 a         1180 c         1172 g          621 t
ORIGIN      
        1 cgatcgccgg gagcgagccc gatggcgtcg cagaagtttt gccagaatcg ctcctcgagc
       61 ggcgcggcgg cgatgaagcg gtcatcgcgc gtgcggtaga cgcggtaacg cgggctgccg
      121 ccggtcacga gcgccttgcc gggctgcggc cagcgccgtg cggcaagtcc gtcgcccatc
      181 gcccagtaca tgaaggtgaa gagattgtcc gccatggcga tgtcgagctt gcagccacgg
      241 ccgctgcgct cgcgttccct caacgccagc aggatattga tcaccgcggg ataggagccg
      301 ccgccgatgt ccgcgaccag cgccgccggc ggcacgggcg cgccgtcggc gcccgccgcc
      361 agcgcgagca tcccggcttc ggcggcgtaa ttcaagtcgt gcgccgccgt cattgcgcgc
      421 gggccggact gcccgtagcc cgtgatcgtg cagtagataa tgcgcgggtt gacgcgcgac
      481 accgcctcgt agcccagccc cagccggtcc attacgccag ggcggaactg ctcgacgatg
      541 atgtcggagc gcttcacgag cggcatcagg cgcccgaccg ccgccggttg cttgagatcg
      601 accgcgatcg atcgttttcc ccggttgagc aacgcgaagt tgacgctgtc ggcgccgaac
      661 ttcggctcgt aactccgcat ctcgtcgccc gcgcccgggc gctcgatctt gatcacttcc
      721 gccccggctt ccgcgagcag gagcgtcgcg agcggccccg gcagcagcgt gctgaagtcg
      781 agtaccctta ttcccgcgag cggttgcatt gtgaacctga gatcaagccg ccgatgtccc
      841 gattttatct gcgttcatcc gcgcttatct gcggttcccg tcgttgtttt gccactcgcg
      901 cgcggcgcgc cggcgcgtac gccgcctcac cgaatgcttc gaacaatgcc tgcgtcacgt
      961 cgaagtgctg ggcgaggccc gtgcgttcca gcagctcgat cggcccctcg ggatagccca
     1021 gcccgagctt gagcgtggta tcgaggtctg cggcgctcgc cagaccctcg tcgagccgcc
     1081 tgagcgcggc gttgtagtac ggccgcacca gccgatcgac gatgcggcct gcgaaatcgt
     1141 tgcacaccgc gacctgcagc cccgcgctct cgaacacctg ccttgccgcc gtgaccgccg
     1201 actcgggtgt ccgcggctgc cggacgagct cgaccaggtt ggaaggcccg gcgcctccca
     1261 gccggaagcg cgcaaaaccg agcacgttcg agccttcgag gccgcgatct tcacccgtgt
     1321 gcgtgcccag gcattcggtc ccgagctcga tcaggacagc ggccctccgg tccgtctttc
     1381 ccaatttgcg gaacgcatct cccgcgttag cgccgacaat cacgacgacc tcggcgatgt
     1441 ccgtcgcact agcgaggaac gggtggtcat cgggaaacga tcggcttact ccggcgcgaa
     1501 taatttggtg tttcatcagc gtttatctgc gtgtatctgt ggctgaggcc gcctgttgtt
     1561 tgtttggatg gtttctattt aagaatgaac gcttgcgttc attcttcatc ctccgaacat
     1621 gctgctgccc tgatacttgt agaaaccttc ccccgttttc cggccgagct tgccggcggc
     1681 aatcatgcgc ttcaccagcg ccggaaccgc cgcgcgcggg tcgcgcgtcg aggcgtagag
     1741 cgcctcggcg accagcaggt gcgtgtcgag cccgacgagg tcgagcagct cgagcggccc
     1801 catcgggtag ccaaggccgg ctttgaccgc gcggtcgatg tcggcgggcg cggcggcgcc
     1861 ggcctcgacc agccggatgg cgtcgttgtt gaacggtatc aggaagtagt tgaggatgaa
     1921 gcccggactg tcctgggtct tcaccggcgt ctggccgagt gccttgcaga aatcccacgc
     1981 ggcctggagc gtcgcctcgc tggtcttcag ccccggcgac atctcgacca gcttcatgag
     2041 ctgcgccggc aggcagaagt gcatgccgac gaagcggtcg tcgcgtcccg agccggcggc
     2101 gatctcggtg atcgagaggg tggaagtgtt ggaagcaaaa atggcatggg gcgggcacac
     2161 cttgtcgagc tgcgcgagca gcccgtgttt cacctcgagg tcctcgaaca ccgcttcgat
     2221 cacgatgtcg caggcggcga gcgccgcgat gtcggtggtg cccgagagat ccgagaggat
     2281 cttcgagacc tgctcctgcg tgagcttctt gcgctcgacc gatttcgcga aaaagccctc
     2341 ggtctgcttg cgcgcccggg ccagcgcttc cgcccgcgtg tcgtagacaa ccgtcctgaa
     2401 tccggcgcgg gcggtgacga tggcgatgcc ggcgcccatg gtgccgctgc cggccacgcc
     2461 tacggttctg atgccgtcac ccgtcactcg tcactcgtca ctggtcacgc atcattcaac
     2521 ggtgatgccg acttccttga tcaccctcgc ccaggcggaa aactcgctct tgatgaacgc
     2581 cgtgaattcc tcgggtgagc tgcccacgac ctccgcaccg acgccgacga tgcgctcctt
     2641 catcgccgtg gccgcgagaa ctttggcgag cgcctggtag atccgccgga ccaccgcggg
     2701 cggcgtgccg gccggaacca tcacgccgtt ccactcgatg gcctcgtagc cgggaacgcc
     2761 ggcctcggcc accgtcggca catcgggcaa cgcgggattg cgctgcgccc ggctcacggc
     2821 gaggccgcgg agcctcccgc cttgtatatg ctggatcgcg gacggaatgg tggcgaagag
     2881 catggaggtt tcaccgctga ccagcgaaat caccgccgga ccgccgcctt tgtagggaac
     2941 gtgagtgagg ttcgtgccgc tcatgtgcat gaacttcgcg gtggcgagat ggttcgcgct
     3001 ggcattgccg gcggatccga agttgagcgc ccccggatgc gccttggcga gcgcgatcaa
     3061 ctgcttcacc gagcgcgccg gcaccgacgg gtgaaccgac agcaccatcg tgacgacggt
     3121 gacttggctg accggtatga gatccctctc cgaattgaac ggcatcttct tcatgaggct
     3181 cgggttggcg ccgaacgtga tgttgggcgt gccccacgta tggccgtcgg gcggcgactt
     3241 cgcggcgaga tcaaggccga tggtggagga accgccgggg cggttctcga ccaccaccgg
     3301 ctggccgagc gcgcgactga gcggctcctg caaggcgcgc gacacgaagt ctgccgcgcc
     3361 gcccggcggg aagccgacca ccagccgcac cgggcgcgtg ggccaagact gggaaaaagc
     3421 agattcctga gcaaaaaaaa catacagcag gaatagccag cgcacgggtc agcgtgccag
     3481 gcgggtgttg gtgagggcca gccggtcgga gacgttgcgc accagcgcgc gcgtgagctg
     3541 cagctg
//
LOCUS       DBNGWZ010000459         4613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2830922, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000459 DBNGWZ010000000
VERSION     DBNGWZ010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2830922"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16CX_14045"
     CDS             complement(<1..352)
                     /locus_tag="AC16CX_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013663.1"
                     /translation="MPEKKEPAYFMYFPGNYRWSAAFINMIGSIAYGGAEIGELHKIG
                     RALKDKGPEDDEAWFKACVKVADGVRAYAETWDKAGHRYAAAHAYLRACNYYQMGERF
                     RTPKDKTGLDAFRKG"
     gene            complement(550..1518)
                     /locus_tag="AC16CX_14050"
     CDS             complement(550..1518)
                     /locus_tag="AC16CX_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013664.1"
                     /translation="MKIVAGLILVFCSLGLSAYGLAQAYPVRPVRLIVPFPPGGPTDT
                     HSRWAAQQLNAAFGQPVVVENRAGAAGVIGTEAVAKAAPDGYTLLGGNPGPLTIAPSV
                     RKQLSYDPVKDFAPITLIARSASCMCVHPSIPARGLKAFVALAKAGPGKINYATPGVG
                     TVGHLAIEHFATLAGIKMTHVPYKGAALYTVDLIAGNMDFAQIQIFQAAPYVRGGKLR
                     ALAVTAVARSPLLPEVPTVQEQGIKGFSSYNWNGILAPAGAPKAVVDRIHAALSRPLT
                     NPDIRKQMEAVGYEVSGDGPAEYAAFIRAETEKWAKVAKAAGIKPE"
     gene            1673..2611
                     /locus_tag="AC16CX_14055"
     CDS             1673..2611
                     /locus_tag="AC16CX_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3013665.1"
                     /translation="MPAKPRKAALKAAPKPAVPSTTRSYQDILYEVKDQVAWVTINRP
                     RVLNAFREQTLDEMIDALKSTREDPSIVCAVVTGAGDKAFSAGGDFFAMKRLNFVNAS
                     MWNDRMLGLAMTIRGLPIPVIAMVNGWCMGGGHELALWCDLVIASENAVLGQTGAKVG
                     ACPTVGATQYMPRILGERLAREMIFCARRFTAKEAVEVGLINKCVPQKDLLKETLKWC
                     ETMKGHSALTLRMTKRSLNFESDNLYASWQHGMELLAHVWGSDEAKEGMNAFLAGRPP
                     NFQKFRMRAKRELDEYLRGLEKNLNEPPIMRRKAKN"
     gene            3178..3561
                     /locus_tag="AC16CX_14060"
     CDS             3178..3561
                     /locus_tag="AC16CX_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013666.1"
                     /translation="MTDSVREHWLETYKSLITISLEGFKFSALANGGAAIALLAYLGN
                     VAGKGVPTPDMREPMFAFLFGLTACGLSMLFAYLTQLKLLNEIGRSERLTFKHGWLLW
                     SAIVLFAGSIVAFGVGSWQAVARFH"
     gene            3677..3961
                     /locus_tag="AC16CX_14065"
     CDS             3677..3961
                     /locus_tag="AC16CX_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013667.1"
                     /translation="MSKLACGFITLLLLSGCASSSGVFRVDDNKYQITTRATWELGGR
                     AGAKRMALEEATRHCESQRKVVRVLRSSEDYGHFEGGTVDMTFSCEQEKQ"
     gene            4081..4575
                     /locus_tag="AC16CX_14070"
     CDS             4081..4575
                     /locus_tag="AC16CX_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013668.1"
                     /translation="MAFRLNAEVLAGRIDAGIFKRQVSVITDGTGVFLPPFPEGTNED
                     LKLGAFNIVLIALSASALTVDETLDQVFGKLSADTNANRVGIRVLVNQLRNAFAHNPW
                     RPKWLVFPKYRNKYPIMLDDGTTFTFNATVLDGDGVKPEHIGGLEFWVKLLQHTERLV
                     DGAV"
BASE COUNT          910 a         1326 c         1457 g          920 t
ORIGIN      
        1 cccccttgcg gaaggcgtcg agcccggtct tgtccttcgg cgtgcggaag cgctcgccca
       61 tctggtagta gttgcaggcg cggagatacg cgtgcgcggc ggcatagcgg tggccggcct
      121 tgtcccaggt ctcggcgtag gcgcgcacgc cgtcggcgac tttcacgcag gccttgaacc
      181 aggcttcatc gtcctccggg cccttgtcct tgagcgcgcg gccgatcttg tgcagctcgc
      241 cgatctccgc tccaccgtag gcgatcgagc cgatcatgtt gatgaacgcc gccgaccagc
      301 ggtagttgcc ggggaagtac atgaagtatg ctggctcttt tttctctggc atggatttct
      361 ctacagttgc gttggctggt ggtggttgac cacgggagat cgtgagatcg ggaaatcggg
      421 agatcgaaat accgggaggt cgggaaatcg ggagatcggg agatcgaaga aagccttgtc
      481 cctctcccgc cctcccgctc tcacggaact tgtcgctctc ccgctctccc gccctcccgc
      541 tctcccgtac tattcgggct tgatgcccgc cgccttcgcc accttcgccc atttctcggt
      601 ctcggccctg atgaacgcgg cgtattccgc cggcccgtcg cccgacactt catagccgac
      661 cgcttccatc tgcttgcgga tgtcggggtt ggtgagcggc ctggagagcg cggcgtggat
      721 acgatcgacg acggccttgg gcgcaccggc gggcgcgagg atgccgttcc agttatagct
      781 ggagaaaccc ttgatgccct gctcctgcac cgtcggcacc tcgggtagaa gaggcgaacg
      841 cgcgacggcg gtgacggcaa gggcgcgcag cttcccgccg cgcacgtagg gcgcggcctg
      901 gaagatctgg atctgggcga agtccatgtt gcccgcgatc agatcgacgg tgtagagcgc
      961 cgcgcccttg tacggcacat gggtcatctt gatcccggcg agcgtcgcga aatgctcgat
     1021 ggcaaggtgc cccaccgtgc ccacgcccgg cgtcgcgtaa ttgatcttac cggggccggc
     1081 cttagcgaga gcgacgaaag ccttcaggcc tcttgccgga atgctcggat gcacgcacat
     1141 gcagctcgcg ctgcgggcga tcagcgtgat cggcgcgaaa tccttgaccg ggtcgtacga
     1201 cagctgcttg cgcacgctgg gggcgatggt gagcgggccg ggattgccgc cgagcagggt
     1261 gtagccgtcc ggggccgctt tcgctacggc ctcggtgccg atcacgccgg cggccccggc
     1321 gcggttttcc acgaccaccg gctggccgaa ggccgcgttc agctgctgcg cggcccagcg
     1381 tgagtgggtg tcggtcggac cgcctggagg aaatggcacg atgaggcgga ccggcctgac
     1441 gggataagcc tgggcgagcc cgtacgcgga gagcccgagc gagcagaaaa cgaggatgag
     1501 tccagcgacg atcttcatgg tgctctcctc ccggtacgac cgcggatgcg ggtagacggt
     1561 cttccgcgcg cttggcacac gatagtagcg cacccgcggt acggcctcaa tgcgatggta
     1621 aactctggcc tccccgcttc gtcatcaaca gccgattcaa tcgaggagtc ccatgcccgc
     1681 caagcccagg aaagcggcgt tgaaagcagc gccaaagccg gccgtcccgt ccacaacccg
     1741 gagctaccag gacatcctct atgaagtgaa ggaccaggtc gcgtgggtca cgatcaaccg
     1801 cccgcgcgtg ttgaatgcgt ttcgcgagca gacgctcgac gagatgatcg acgccctcaa
     1861 gtccacccgt gaggatccct cgatcgtgtg cgcggtggtg accggcgcgg gcgacaaggc
     1921 gttctcggcg ggaggcgact ttttcgccat gaagcgattg aacttcgtga acgcctccat
     1981 gtggaacgac cgcatgctgg ggctcgcgat gacgatccgg gggctgccga tcccggtgat
     2041 cgcgatggtg aacggctggt gcatgggcgg cggtcatgag ctcgcgttgt ggtgcgacct
     2101 ggtgatcgcc tccgagaatg cggtgctcgg ccagaccggc gccaaggtgg gcgcctgccc
     2161 gacggtcggc gccacgcagt acatgccgcg catcctgggc gagcggctcg cgcgcgagat
     2221 gatcttttgc gcgcggcgct tcaccgcgaa ggaggcggtg gaagtcggcc tcatcaacaa
     2281 atgcgtgccg cagaaggacc tgctcaagga aacgctcaag tggtgcgaga ccatgaaggg
     2341 gcacagcgcg ttgacgctgc gcatgaccaa gcgctcgctc aacttcgagt ccgacaacct
     2401 ctatgcgtcc tggcagcacg gcatggagct gctcgcgcac gtctggggat ccgacgaggc
     2461 gaaggagggc atgaacgcgt tcctcgccgg gcggccgccc aacttccaga agttccggat
     2521 gcgggccaag cgcgagctcg acgaatacct gcgcgggctg gagaagaacc tgaacgagcc
     2581 gccgatcatg cgcaggaagg cgaaaaactg aaccgccaag gcccaaagga agagaagacg
     2641 ggagagcgtg agggcgtgag agcgggagag cgacaaaatc cgtgagtgcg tgagagcgtc
     2701 agagcgtaag tgcgacaatc catctcccga tctcacgatt tcccgatctc acgggttttg
     2761 ggaaccgcca agagcgccaa gcacgccaag aaaatcaaca acaatcagaa tcaaaccgca
     2821 agagagcggt acggcggcat gatatttggc gatttcgctg actattggat gttaggcact
     2881 taggagcaca gaatgagccg agtagcatgg gttggagttt ctctgctagt cgcggcatgt
     2941 gcagctatcg agccgcagca attcgtcggt cctagtggca agattgctta ttccatgcgc
     3001 tgtagcggca tggggcgcac tctcgacgcc tgttacaaga aggcgggaga ggtttgccct
     3061 ggcggataca cgatcattga tcgcgcgtct ggcacggtcg cggttccggt tcagggtggg
     3121 attattgcgg ccccacagca caaccttgcg atcgagtgca aatagggcac atcgtcaatg
     3181 accgactcag tgcgggagca ttggctagaa acatacaagt ccctcattac catttcactt
     3241 gagggcttca agttttccgc gctcgccaac ggtggggcgg ccatagccct gctcgcttat
     3301 ctcggcaatg tggccggcaa gggcgtgcca acgcctgata tgcgcgaacc aatgttcgct
     3361 ttcttatttg ggcttacggc atgcggtctt tcgatgcttt ttgcgtacct gacacaactg
     3421 aagctactca acgagattgg ccgctcagag aggttgacgt tcaaacatgg gtggctcctc
     3481 tggagcgcga ttgtgctatt cgctggcagt atcgttgcgt ttggcgttgg atcatggcag
     3541 gcagtcgctc gctttcattg atcgtgccta accgccgcat caacgccgac cgcaatgcgg
     3601 tgggttatgc gggacgttag aggtttcaat atttactcaa tccgaagcga ctactcatag
     3661 ccatttggag tatttcatgt ccaagctcgc atgcggtttc ataactcttc tattgttgtc
     3721 aggctgcgcc agtagttcgg gcgtatttcg tgtggatgac aacaaatacc agatcacgac
     3781 gcgggctact tgggagttgg gcgggcgtgc tggtgcaaag cgtatggcac tcgaagaagc
     3841 aactcgccac tgtgagtctc aacggaaagt cgtgcgcgtc cttcgatcgt ctgaagacta
     3901 cggacacttc gagggcggta cggtcgatat gacttttagc tgtgaacaag aaaaacagtg
     3961 agatggtcgg gccccacctc taactttccg gttacctcca agtgttagac cgctatgacg
     4021 aacggtcacg gtgcagagac tccggcaaac aagatagcta cggcccaggc ggttctcagc
     4081 ttggcgtttc gcttaaatgc cgaggtgttg gctggtcgga tcgatgcagg gatattcaag
     4141 cgccaagtca gtgtcatcac tgatggcact ggagtctttc tcccgccatt tcctgaaggc
     4201 acaaatgaag atctcaagct cggagcgttc aatatcgttc tcatagcgtt aagtgcttct
     4261 gcgcttacgg tcgacgaaac gctcgaccaa gtatttggca agctgtcagc tgacactaac
     4321 gccaatcggg ttggcatcag agttctggtt aaccaacttc gcaatgcgtt cgctcacaac
     4381 ccatggcgtc cgaagtggct tgtttttccc aaatacagaa acaagtatcc aatcatgctt
     4441 gacgacggta cgacgttcac gtttaacgct acagtactcg acggcgatgg cgtcaaaccg
     4501 gagcacattg gaggcttgga gttctgggtt aagctgttgc agcacaccga aagactggtc
     4561 gatggagcgg tctgacccca cagtcgagag gagcgtgcgg aagcggtctt tcg
//
LOCUS       DBNGWZ010000460         6487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1571737, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000460 DBNGWZ010000000
VERSION     DBNGWZ010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1571737"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /gene="typA"
                     /locus_tag="AC16CX_14075"
     CDS             <1..1777
                     /gene="typA"
                     /locus_tag="AC16CX_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR3013669.1"
                     /translation="DHGKTTLVDQLLRQSGTFAAHQHLVERVMDSNDLERERGITILA
                     KNCAIDYAGTHINIVDTPGHADFGGEVERVLSMVDGVLLLVDAVEGPMPQTRFVTRKA
                     LAQGLKPIVVVNKVDRPGARAEWVVNHTFDLFDKLGATEAQLDFPVVYASALQGWATT
                     RSGETGTDMKPLFEAILAQVPVRADDPDGPLQLQICSLDYSSYVGRIGIGRINRGRIR
                     SGQPVTVLRGPDSAPVMAKINQVLVFKGLERVVTDEAQAGDIVAINGVEEIGIGVTLA
                     DPERPEALPLLKVDEPTLVMNFQVNTSPFAGREGRFVTSRQLRERLAREVMGNVALRV
                     EDTADPDVFKVSGRGELHLTILLENMRREGYELAVSRPRVLMKVISGIQCEPFELLAV
                     DVEETHQGAVMEALGARRGDLQDMQPDGKGRVRLDYRIPARGLIGFQSEFLTMTRGTG
                     IMSHVFDDYGPMKPEITERRNGVLISAEDGEAVAYALWKLQERGRMFVDPGEPLYEGM
                     IVGLHTRDNDLVVNPVKTKQLTNIRAAGKDEAILLTPPIRHTLESAVEFIADDELVEI
                     TPKSIRIRKRLLLEHERKRASRVAA"
     gene            complement(1976..3358)
                     /locus_tag="AC16CX_14080"
     CDS             complement(1976..3358)
                     /locus_tag="AC16CX_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3013670.1"
                     /translation="MSVFFAPPRGTMLARLGEAMRRHRRFILAGQWMVVVVYLVLLTV
                     PAFAPLPPAGASILSNLTLFAQFAFWGIWWPFVMLSMMLVGRVWCGVLCPEGALSEWA
                     SRHGLGKSIPRWMRWSGWPFAAFVMTTVYGQLVSVYEYPKAALLVLGGSTVAAVVIGF
                     LYGKGKRVWCRHLCPANGVFALLAKIAPVHFRTNEEAWKRFPGRAASVDCAPLVDLRH
                     LHSASQCHACGRCSDHRSAIELGARSPNREILSLAPQPDAGAAAALLIFGVLGVALGA
                     FQWTLSPWFVAMKLASAEWLIERDSLWLLQDNAPWWILTHYPEASDVFNWLDGLCIVA
                     YIFGTAIVVGTATLGALLGAQFLAGRGRYDWKSLAMTLIPIGGIGVFLGLSMLTLGQL
                     RAEGVTFAWLGEVRAVLLLLGLGWSGWLGTRLVFASPVPRLRRLAALALHTLPVALIG
                     YSWYLAFYVW"
     gene            complement(3378..3716)
                     /locus_tag="AC16CX_14085"
     CDS             complement(3378..3716)
                     /locus_tag="AC16CX_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3013671.1"
                     /translation="MAQNLRRALVLLATVALTAAATAQDLPVFRIVMKDGRFSPERLE
                     VPAGKRLKLVLKNEGRGPAEFENLSMRVEKVLAPGVTSFVVLHALKPGEYRFVDEFRP
                     DGGSLTLVAK"
     gene            complement(3807..4355)
                     /locus_tag="AC16CX_14090"
     CDS             complement(3807..4355)
                     /locus_tag="AC16CX_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HIR3013672.1"
                     /translation="MSLLRPAGVALVSLAALILHSAAAIEYPIGTPRNVAGMEIAAVY
                     LQPIEMEPEGMMAKASESDIHLEADIHALANNANGFPEGTWIPHLVVKYELTKQGSNE
                     KIAGEMMPMVASDGPHYGANVKLRGPGRYRLKYFIAPPSANKQVYFGRHTDRATGVRP
                     WFKPFEVQYEFTFAGVGKKGGY"
     gene            complement(4417..5262)
                     /locus_tag="AC16CX_14095"
     CDS             complement(4417..5262)
                     /locus_tag="AC16CX_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR3013673.1"
                     /translation="MFGSAIVVFRETLEAALIIAIVMGASRGIAARGRWITGGAALGI
                     AGALVVALFAGALAQAVEGRGQELFNAGVLLAAVAMLAWHNIWMSSHARKHVAQMQHL
                     GHDVRVGLKPLSAMLVVTALAVLREGAETVLFLYGLWAGGGGRGELALGAMVGLSAGA
                     LVGVLLYLGLLRIPLRHFFTVTGLIVLLLAAGLAAGAAGYLTQAGLLEPISEQVWDTS
                     SLLSVESFPGRVLHVLVGYRDRPTGIELLFYAVTFITIAALMRIVGARAARVARLEAK
                     AGSSA"
     gene            5727..5924
                     /locus_tag="AC16CX_14100"
     CDS             5727..5924
                     /locus_tag="AC16CX_14100"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3013674.1"
                     /translation="MYVCICHAVTNCKIRACIEGGADSMRDLREHLAVGTQCGKCARH
                     VREILKGEQKVGLQNYIQSQM"
     gene            6011..>6487
                     /locus_tag="AC16CX_14105"
     CDS             6011..>6487
                     /locus_tag="AC16CX_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HIR3013675.1"
                     /translation="MPFDMNLIQRGARLRRSPFFEATQRHGAKAYTVYNHMLFPICFG
                     DLEEEYWHLLNHVTLWDVSVERQVEITGPDAFTFTNMLTPRDLSRCAVGQGKYVVITA
                     EDGGIINDPVLLRLGENHFWLALADSDVLLWARGVALNSGLHVSITEPDVSPLQIQG"
BASE COUNT         1262 a         2116 c         2057 g         1052 t
ORIGIN      
        1 cgaccacggc aagaccacgc tggtggacca gctgctgcgc cagtccggca ccttcgccgc
       61 gcaccagcac ctggtcgagc gcgtgatgga ctcgaacgac ctcgagcgcg agcgcggcat
      121 caccatcctt gccaagaact gcgcgatcga ctacgccggc acgcatatca acatcgtgga
      181 cacgccgggc cacgccgact tcggcggcga ggtcgagcgg gtgctgtcga tggtggacgg
      241 cgtgctgctg ctggtggacg cggtcgaggg cccgatgccg caaacgcgct tcgtcacgcg
      301 caaggcgctg gcgcaggggc tcaagcccat cgtcgtggtg aacaaggtgg accgccccgg
      361 cgcgcgcgcc gagtgggtcg tcaatcacac cttcgatctg ttcgacaagc tcggcgcgac
      421 cgaagcccag cttgatttcc cggtcgtgta cgcgtccgca cttcaaggct gggccacgac
      481 gcggtccggg gaaaccggaa cggacatgaa gccgctgttc gaggcgatcc tcgcgcaggt
      541 gccggtgcgc gcggacgatc ccgacggtcc gctgcagctg cagatctgct cgctcgatta
      601 ctcgagctac gtcgggcgca tcggcatcgg gcgcatcaac cgcggacgca tccgctccgg
      661 gcagccggtg acggtgctac gcggccccga cagtgcgccg gtgatggcca agatcaatca
      721 ggtgctggtg ttcaaggggc tcgagcgcgt cgtcaccgac gaggcgcagg ccggcgacat
      781 cgtcgccatc aacggcgtcg aggaaatcgg catcggtgtc acgcttgccg accccgagcg
      841 cccggaagcg ctgccgctgt tgaaggtgga cgaaccgacg ctcgtgatga atttccaggt
      901 gaacacctcg cctttcgccg ggcgtgaagg ccgcttcgtc accagccgtc aattgcgcga
      961 gcggctcgcg cgcgaagtga tgggcaacgt cgcgctgcgc gtcgaggaca ccgccgatcc
     1021 ggacgtgttc aaggtgtccg ggcgcggcga gctgcacctc accatcctgc tcgagaacat
     1081 gcgccgcgaa ggctacgagc tcgcggtatc gcgcccgcgc gtgctgatga aagtgatcag
     1141 cggcatccaa tgcgagccgt tcgaactgct cgccgtggac gtcgaggaaa cgcaccaggg
     1201 cgcggtgatg gaagcgctcg gcgcacgccg cggtgacctt caggacatgc agcctgacgg
     1261 caagggccgc gtccgcctcg attaccgtat cccggcccgt ggcttgatcg ggtttcagtc
     1321 cgagttcctc acgatgacgc gcggcaccgg catcatgagc cacgtgttcg acgattacgg
     1381 cccgatgaag ccggagatca ccgagcggcg caatggtgtg ctcatttcag ccgaggatgg
     1441 cgaagcggtc gcctacgcgc tgtggaagct gcaggagcgc ggccgcatgt tcgtggaccc
     1501 cggcgagccg ctctacgagg gcatgatcgt cggactgcac acccgcgaca acgacctagt
     1561 cgttaatccg gtgaagacca agcagctcac caacatccgc gccgccggca aggacgaggc
     1621 gatcctgctg acgcccccga tccgccacac gctggaatcc gccgtcgaat tcatcgccga
     1681 cgacgagctg gtcgagatca ccccgaagtc catccgcatc cgcaagcgcc tcctgctgga
     1741 acacgagcgc aaacgcgcat cacgcgttgc ggcgtaatcg cttatcaggc gtgccgacgc
     1801 cgggatcgat gccagcttcg cgataacgac actcagcaca aacgtttgtt tgctgatcgt
     1861 gtgcggccag gaacgcaacc ggcaccaacg cctggcacag tgcaccggcg ttggttctag
     1921 cggcttgggt cgcggctttg taacccgttt cggcggcgga atcatggcgc aacgactacc
     1981 agacgtagaa cgccaggtac caggaatagc cgatcagtgc gaccggcagc gtgtggagcg
     2041 cgagcgctgc gagccggcgc aaacgaggca ccgggctcgc gaatacgagg cgcgtaccca
     2101 accaccccga ccagcctagg ccgaggagaa gcagcacggc gcgcacttcg ccgagccaag
     2161 caaatgtgac cccctcggca cgcaactggc cgagggtcag catcgacagc ccgagaaaca
     2221 cgccgatgcc gccgatcgga atgagcgtca tcgcgagcga cttccagtcg tagcgcccgc
     2281 ggcccgccag aaactgcgct ccgagcagcg cgcccagcgt cgctgtgccg acgacgatcg
     2341 ccgtgccgaa gatgtacgcg acgatgcaca ggccgtcgag ccagttgaag acgtcgctcg
     2401 cctccgggta atgggtcagg atccaccagg gcgcgttgtc ctggagcagc cagagcgagt
     2461 cgcgctcgat cagccattcg gccgacgcca gtttcatcgc gacgaaccag gggctcagcg
     2521 tccactggaa cgcgccgagc gccacgccga gcacgccaaa gatcagcagc gcggcagccg
     2581 cgccggcatc gggttgcggg gcgagcgaca ggatttctcg gttgggagac cgggcgccaa
     2641 gttcgatcgc gctccggtgg tcactgcagc ggccgcacgc gtggcactgg gaagcgctgt
     2701 gcaggtggcg caggtccacg agcggcgcgc aatcgacgct cgcggcacgc cccggaaagc
     2761 gtttccacgc ctcctcgttt gtgcgaaagt gcaccggcgc gattttcgcc agcagcgcga
     2821 acacgccgtt cgccggacag aggtggcggc accagacgcg cttgcccttg ccgtagagga
     2881 agccaatgac gacagccgct accgtcgagc cgcccagcac cagaagcgcc gctttgggat
     2941 actcgtagac gctcacgagc tggccataca ccgtcgtcat gacgaacgcc gcgaagggcc
     3001 acccgctcca gcgcatccag cggggaatcg actttcccag accgtggcgg ctcgcccact
     3061 cgctgagcgc gccctcggga cacagcacgc cgcaccagac ccggcccacg agcatcatgc
     3121 tcagcatgac gaatggccac cagatgcccc agaacgcaaa ctgggcgaac agcgtgagat
     3181 tggacagaat gctcgcgccg gcgggcggca acggcgcgaa cgcgggcacc gtcaagagaa
     3241 ccaggtaaac gacgaccacc atccattgac cggcaagaat gaaacggcgg tggcggcgca
     3301 tcgcctcgcc cagtcgcgcc agcatggtgc cgcggggcgg agcgaagaag acgctcactg
     3361 aatcagccat gatccgatca cttcgcgacg agcgtcaggc ttccgccgtc ggggcgaaac
     3421 tcgtcgacga aacggtattc gcccggcttc agggcgtgca gcaccacaaa ggaggtgacg
     3481 cccggcgcga gaaccttttc cactcgcatg ctcaggttct cgaactcggc ggggccgcgg
     3541 ccttcgtttt tcagcaccag cttgaggcgc ttgcctgccg gtacttccag ccgctcggga
     3601 gaaaagcggc cgtctttcat cacgatccga aagacgggca agtcctgcgc cgtcgccgcg
     3661 gcggtgagcg ccaccgttgc gagcaggacc agcgcgcggc gaaggttttg cgccatgacg
     3721 ttcaatatcc gactcttgct ctcagcgcga cgacgggcac ggacccgctg aacgtcagaa
     3781 cgggcggtgt agcagacact gccatctcag tacccgccct tcttgccgac gccagcgaaa
     3841 gtgaattcgt attgcacctc gaacggcttg aaccagggac gcacgccggt ggcgcgatcc
     3901 gtatggcggc cgaagtagac ctgcttgttg gcgctgggcg gcgcgatgaa atacttgagc
     3961 ctgtatctgc ccggacccct gagcttgacg ttggcgccat agtgcgggcc gtcgctcgcc
     4021 accatgggca tcatttcgcc tgcgattttt tcgttgctgc cctgctttgt aagctcgtat
     4081 ttcacgacca agtgcggaat ccaggtgcct tcggggaagc cgttggcgtt gttcgcaaga
     4141 gcatggatgt cagcctcgag atggatgtcc gattccgagg ccttggccat catgccctcg
     4201 ggctccatct cgatcggctg caggtaaacg gccgcgattt ccattcccgc cacgttgcgt
     4261 ggggtgccga tcgggtattc gatcgcggcg gcggaatgca ggatgagcgc agcgagcgac
     4321 acgagcgcga cgccggcggg acgaagcaac gacatgaatt ttctcctgag gtctggtttt
     4381 cggatgactt cccggtctgc gcgaacgcga gagcggtcat gcggaactcc cggctttcgc
     4441 ttccaggcgc gcaacgcgcg ccgcgcgcgc gcccacgatc cgcatgagtg ccgcgatggt
     4501 gatgaacgtc acggcgtaaa agagcagctc aatgccggtc ggacggtccc ggtagccgac
     4561 aagcacgtgc aggacgcgac cgggaaaact ctcgacgctg agcaagctgg aagtgtccca
     4621 gacctgctcg gagatcggct caagcagtcc ggcctgcgtg aggtagccgg cggcgcccgc
     4681 ggcaagcccc gccgcgagca acagcacgat gagtccggtg accgtgaaaa agtgccgcaa
     4741 tggtattctg agcagaccca gatagagcag gacgccgaca agcgcgcccg cgctcaggcc
     4801 caccatcgcg ccgagcgcaa gctcaccgcg tccgccgccg cccgcccaca gaccgtacag
     4861 gaagagcacc gtttcggcgc cctcgcgcag gacggcgagc gcggtcacga cgagcatggc
     4921 cgaaagcggc ttgaggccga cacgcacgtc gtgcccgaga tgctgcatct gggcgacgtg
     4981 cttgcgcgcg tgcgaactca tccagatgtt gtgccaggcg agcatcgcga cggctgcgag
     5041 caggacgcca gcgttgaaca gttcttggcc gcgtccttcc actgcctgtg cgagcgcacc
     5101 agcgaacagc gcgaccacca gcgcgccggc gattccgaga gcggcgccgc cggtgatcca
     5161 ccgcccgcgc gccgcaatgc cgcggcttgc gcccatgacg atcgcaatga tcagcgcggc
     5221 ttcaagcgtt tcgcggaaaa cgacgatggc agatccgaac atggttactc gtcctcgttc
     5281 ctcactttgc cgccttccac cgcttccggg taatccacac cggtgtaatc gaacaggtgc
     5341 agaatcgttt gcgcgggatt ctcttgcgag atcaccggca tcgcggacag cgcgaacgcc
     5401 agtagtaggc accgcgcaac cgaaaatgaa tgaacgcggc caggagtaca gcgtgtaacg
     5461 cggtcacagc tatcaacacg atcgaagatt gcttgcgatc cctatgaacc attggttttg
     5521 ctggctgtgg agagcgaccg ttatggctaa ccgtgtgccc tacaggcatc tttcgaagct
     5581 caattccttg atcgggcagg atattcctgt ctcaaaaggt ctctagagag gtagaatatc
     5641 acggatgaga atgattcgca atactattta caacgatgtt ccttttggct atatttcgag
     5701 cgtccggacg ccatcctcta agacttatgt acgtttgcat ctgccatgcc gtgaccaatt
     5761 gcaaaatccg cgcctgtata gagggtggcg cggattcgat gcgcgatctg cgcgagcacc
     5821 tcgccgtcgg cacgcaatgc ggcaaatgcg cccgtcacgt gcgagaaatc ctcaagggtg
     5881 agcagaaagt cggtttgcag aactacattc agtcccagat gtaaggcatt gcggggcttc
     5941 aggaccgcga caagccggcc taggcaaggg atatcattgc ccttcatcca ttacctgccc
     6001 cgatcccgca atgcctttcg acatgaatct catccagcgc ggcgcgcgcc tgcgccggag
     6061 cccgttcttc gaagccacgc aacgccacgg cgcgaaggct tacaccgttt ataaccacat
     6121 gctgttcccg atctgcttcg gcgacctgga ggaggagtac tggcatctgc tcaaccacgt
     6181 cacgctgtgg gacgtctcgg tcgagcggca ggtcgagatc accgggccgg acgcgttcac
     6241 cttcaccaac atgttgacgc cgcgcgacct ctcgcgctgc gccgtgggcc agggcaagta
     6301 cgtggtgatc acggccgagg acggcggcat catcaacgac ccggtgctgc tgcgcctggg
     6361 cgagaaccac ttctggctcg cgctcgccga cagcgacgtg ctgctgtggg cacgcggcgt
     6421 agcactcaat tctggcctgc atgtgagcat cacagagccc gacgtctcgc ccctgcagat
     6481 ccagggc
//
LOCUS       DBNGWZ010000461         2606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1947965, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000461 DBNGWZ010000000
VERSION     DBNGWZ010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1947965"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC16CX_14110"
     CDS             complement(<1..929)
                     /locus_tag="AC16CX_14110"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3013676.1"
                     /translation="MSDIDALHPLDFDAPRLTVKRPAVRHDAQGELSDSERAARAMAA
                     FTRDMSSTVATYMESCIHCGHCAEACHFFQVSQEPQYTPIRKLEPFKQAYQREAGPFA
                     WFYRTFDLKPAVTAQELREWQELLYDSCTMCGRCTLACPMAIDIAGLVALARHAMFAA
                     GLVPHELWAVAERAEREMSPLGATPKVLRERVEWLADEHEVEIPFDRERADVLMTLSS
                     IEIMKYPASIVSVARVMRHAGISWTLRSDGYEATNFGLFSGNLEWQRDMSMRLINAAV
                     ACGARLLVLPECGHAYGALRWQGANLYGKPLPFR"
     gene            complement(926..1609)
                     /locus_tag="AC16CX_14115"
     CDS             complement(926..1609)
                     /locus_tag="AC16CX_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013677.1"
                     /translation="MTLLEFARGPALTFSAAVFLLGVAWRLYGIFRRPARVDLSEPRR
                     TDTVAGALRAILSRMFPRREFWKAGAFQTVNAYAYHLGLFFIVFTFAPHIGFIERMLG
                     PSWTALPDVVTYVATAITVVALLIALLMRLTSPVQKLLSGFDDYFTWFVTLLPILTGM
                     GLIERRFVPEPLVAPALPTTPELLAVHLLSLELMLVWFPFGKLMHAVLVFVSRARTGA
                     EFTRKGAAV"
     gene            1969..>2606
                     /locus_tag="AC16CX_14120"
     CDS             1969..>2606
                     /locus_tag="AC16CX_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR3013678.1"
                     /translation="MSDSNRQGRAMHEIESWSEEKRSAHLYRVVAESEAGTARHALFS
                     ELAREAEKQAAIWERLARAAGREVPAGFVPDLRTRLVAALVRRFGARPLRGVLAAMKV
                     RGMSLYSTAAHGHALPRSVEEVGGRHRGVESGGNLRAAVFGVNDGLISNASLIMGIAG
                     ATADNQIVLLSGAAGLIAGAFSMAAGEYVSVRSQREMYEYQIGLEREELEHYP"
BASE COUNT          462 a          836 c          903 g          405 t
ORIGIN      
        1 cggaacggca gcggcttgcc gtacaggttc gcgccctgcc agcgcagcgc gccgtatgcg
       61 tgcccgcact ccggcagcac gagcagtcgc gccccgcacg ccactgcggc gttgatcagc
      121 cgcatgctca tgtcgcgttg ccactcgagg ttgcccgaga acagcccgaa gttggttgcc
      181 tcatagccgt cgctgcgcag agtccaggag atgccggcgt ggcgcatgac gcgggcgacc
      241 gagacgatgg aggccgggta cttcatgatc tcgatcgagg acagcgtcat cagcacgtcg
      301 gcgcgctcgc ggtcgaaggg gatctccacc tcgtgctcgt cggccagcca ttcgacgcgc
      361 tcgcgcagca ctttgggcgt ggcgccgagc gggctcatct cgcgctcggc gcgctcggcg
      421 accgcccaca gctcgtgcgg gacgagtccc gcggcgaaca tcgcgtgccg cgccagtgcg
      481 accagccccg cgatgtcaat cgccatcggg cacgccagcg tacagcgccc gcacatcgtg
      541 cacgagtcgt agagcagctc ctgccactcg cgcagctcct gggccgtgac cgccggcttc
      601 aggtcgaacg tgcggtagaa ccaggcgaac ggcccagcct cgcgctggta cgcctgcttg
      661 aacggctcga gtttccggat cggcgtgtat tggggctcct ggctgacctg gaagaagtgg
      721 cacgcttcgg cgcaatggcc gcaatggatg cacgattcca tgtaggtggc gacggtggag
      781 ctcatgtcgc gggtgaacgc ggccatggcg cgagcggcac gctcgctgtc gctcaactcg
      841 ccctgggcgt cgtggcgcac cgcgggccgc ttgaccgtga gccgcggcgc gtcgaaatcg
      901 agcgggtgca aggcgtcgat gtcgctcata cggcggctcc cttgcgcgtg aactcggcgc
      961 cggtgcgggc gcgcgagacg aagaccaaca ccgcgtgcat gagcttgccg aacgggaacc
     1021 acaccagcat cagctcgagg ctcagcaggt gcacggcaag cagctccggc gtggtcggca
     1081 acgccggagc gacgagcggt tccggcacga aacggcgctc gatcaagccc atgcccgtga
     1141 gaatcggcag cagggtcacg aaccaggtga aatagtcgtc gaagccggac aacagctttt
     1201 gcacggggct ggtgaggcgc atcagcagcg cgatcaggag cgcgacgacc gtgatcgcgg
     1261 ttgccacgta agtgacgacg tcgggcaatg cggtccagga gggaccgagc atgcgctcga
     1321 tgaagccgat gtgcggggcg aacgtgaaga cgatgaagaa caaaccgagg tggtaggcat
     1381 aggcattcac cgtctggaac gctcccgctt tccagaactc acgccgcgga aacatccgcg
     1441 agagaatcgc gcggagcgcc ccggccaccg tatcggtgcg gcggggctcg gaaagatcca
     1501 ccctggccgg ccgcctgaaa atcccgtaca gacgccacgc gacgccgagg agaaacacgg
     1561 cggcggaaaa ggtgagcgcc gggccgcgcg cgaattcgag caacgtcaag atcaggacct
     1621 ttgttgtcgg ggcgtgccgc ggccttattc catccgattt tcgaccgcgg cgtttgattt
     1681 ggatcaagcg gttcaccgtc tgtcggcgtg ccgcccggcg gcagccctag gagtttgtcg
     1741 gacttagctc ggacaaactc ctaatgcaaa accgcccgca cgaaaactga agcaaaaggc
     1801 taaaacctca tttctcgcat aatcgagtac ccatcggagg ctaatcgggg ccgtcactct
     1861 gggctttatt cgaaatccac aggcggtttt gctattagca gcctgtcgga cacctgaagc
     1921 cgacaggctg ctagttgttg ccggccggtc gaatgtcgtg gatgattcgt gtcggattcg
     1981 aaccggcagg gacgcgccat gcacgagatc gagagctggt cggaggagaa acgctcggcc
     2041 catctctatc gcgtcgtggc ggagagcgaa gccggaacgg cccgtcacgc gttgttctcg
     2101 gagctggcgc gggaggccga aaagcaggcg gcgatctggg agcggctcgc gcgcgccgcg
     2161 gggcgcgagg tccccgccgg gttcgttccc gatctgcgca cgcggctcgt cgccgcgctg
     2221 gtgcggcgct tcggagcgcg gccgctgcgc ggcgtgctgg cggcgatgaa ggtgcgcggg
     2281 atgtcgctct actcgaccgc cgcgcacggc cacgcgctgc cgcgctcggt ggaggaggtc
     2341 ggcgggcgcc accgcggcgt ggaaagcggc gggaatctcc gcgccgccgt gttcggcgtc
     2401 aacgacgggc tgatctccaa cgcgagcctc atcatgggca tcgccggcgc caccgccgac
     2461 aaccagatcg ttctcctttc gggcgccgca ggcctcatcg cgggggcgtt ctcgatggcg
     2521 gccggcgagt acgtctcggt gcgctcgcag cgcgagatgt acgagtacca gatcgggctc
     2581 gagcgcgagg agctcgaaca ctaccc
//
LOCUS       DBNGWZ010000462         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2831326, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000462 DBNGWZ010000000
VERSION     DBNGWZ010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2831326"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="AC16CX_14125"
     CDS             <1..894
                     /locus_tag="AC16CX_14125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013679.1"
                     /translation="YPAGPVRVIVPFPPGGGVDGMARIVCQKLSESLGKTFPIENRSG
                     ANGNIGTELAAKAPKDGYTLLFTGAGLVTNPSLYSKVPYDPVRDFEPISLLSIAPNVL
                     VVHPSLPAKSVKELAALARARPGQVLFAGSGSGSTPHLAAELFNALAKVKMVHVPFRG
                     TGPAIIGLMSGEASVMFMPSTNAVQLVRSGRLRALAVTSRERLAALPELPTVAESGLK
                     GYESSQWYGALAPAGAPADVLTLLNSHIAKIMQSPDMKQRLGAEGVIPVGSTREQFAS
                     HIKVETVKWARVVKESGARVD"
     gene            980..1975
                     /locus_tag="AC16CX_14130"
     CDS             980..1975
                     /locus_tag="AC16CX_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013680.1"
                     /translation="MRSNPALMMKASHAMAALAIASSLAYANDAAAQAFPSRPIRMIV
                     PQSAGGSTDVIARVVAQKLSDALGQTVVVDNRPGAGSINGTEMVANATPDGHTLLAVA
                     ASFTINPALHKKLPFDPVRDFAPITQVATLPHILIVHPSVPVKSVKELIALIKSKPGE
                     LNYASSGVATSTHLAAELFMYMTGTKMVNVPYKGGSPAMTAMLGGQCQVNFATISTAL
                     PHVRSGKIRALAVTSAKRSVAAPEYPTIAEAGVPGYSHASWVGLLAPAKTPQSIIASL
                     NAEAVKAAHSQDVKAVLLRDGLEATGTTPGEYSAIIKSEVAKWFKVVKAAGIVAQ"
     gene            2037..>2590
                     /locus_tag="AC16CX_14135"
     CDS             2037..>2590
                     /locus_tag="AC16CX_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012804.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3013681.1"
                     /translation="MSLVEDLVVAYRTLAQEGVIDAYGHVSVRSEKDPNRYLMARQLA
                     PELVTEEDILEFDLDSNPIDPRGRKFYNERYIHGEVYKARPDVMAVVHNHAPAVVPFS
                     CTSRSSLKPIFHMSAFIGMGVPNWDIREAQKGSDMLVRTPYLGQSLAKKLGKYPAVLM
                     RGHGATVVGENLQRAVGRGVYLEV"
BASE COUNT          495 a          853 c          834 g          408 t
ORIGIN      
        1 taccccgccg gcccggtgcg cgtgatcgtg cccttcccgc cgggcggcgg cgtggacggc
       61 atggcccgca tcgtctgcca gaagctgagc gaaagcctcg gcaagacctt cccgatcgag
      121 aaccgcagcg gcgcaaacgg caatatcggc accgagcttg ccgccaaggc gcccaaggac
      181 ggctacacgc tgctcttcac cggcgcgggg ctcgtcacca acccgagtct ctacagcaag
      241 gtcccctatg acccggtcag ggacttcgag ccgatcagcc tgctctccat cgcgcccaac
      301 gttctggtcg tgcacccgtc gcttccggcg aaatcggtaa aggagctcgc cgccctcgcg
      361 cgagcgcgcc cggggcaggt gctgttcgcg ggctcgggca gcggcagcac gccgcacctg
      421 gccgcggagt tgttcaacgc gctcgcgaag gtcaagatgg tgcacgtccc tttccgcgga
      481 accggccccg cgatcatcgg tttgatgagc ggggaagcca gcgtcatgtt catgccctcg
      541 accaacgccg tgcagctcgt caggtcaggc cggctaaggg cgctcgcggt gaccagccgg
      601 gagcggctcg cggccctgcc cgagctgccg accgtcgcgg agtccggtct gaagggttac
      661 gaatcgagcc agtggtacgg cgcactcgct cccgccggcg cgccggccga tgtgctcacc
      721 ctgctcaact cgcacatcgc gaaaatcatg caaagcccgg acatgaagca gcgcctaggc
      781 gccgagggag tcattccggt cggcagcacg cgcgagcaat tcgcatcgca catcaaggtc
      841 gagaccgtca aatgggccag ggtcgtcaag gagtccgggg cacgggtcga ttgagggatg
      901 cgagatacgg gatccgggat tcgtgatgtg ggataagtgc tggactcaga ccaaaaagag
      961 ctgcttcgag gagatgaaaa tgcgatcgaa cccggcactc atgatgaaag cgagccacgc
     1021 aatggccgct ttggccattg catcgtcctt ggcatatgcc aatgacgcgg cggcgcaggc
     1081 ttttccgagc cggccgatac gaatgatcgt ccctcagagc gcgggcggtt ccaccgacgt
     1141 gatcgcgcgc gtcgtggcgc aaaagctgag cgacgcgctt ggccagaccg tggtcgtgga
     1201 caaccgtccc ggggcgggca gcatcaacgg cacggaaatg gtggccaacg cgacgccgga
     1261 cggtcacacg ctgctcgccg tcgcagcctc cttcaccatc aatcccgccc tgcacaagaa
     1321 gctgcccttc gatccggtcc gcgatttcgc gccgatcacg caggtggcga ccctgcccca
     1381 catcctgatc gtccacccgt cggtcccggt gaagtcggta aaggagctga tcgcgcttat
     1441 taaatccaag cctggcgagc tcaactacgc ctccagcggg gtggcgacga gcacgcacct
     1501 ggcggccgag ctgttcatgt acatgaccgg caccaaaatg gtgaacgtcc cgtacaaggg
     1561 cggctcgccc gcgatgaccg cgatgctcgg cggccagtgc caggtcaact tcgcgacgat
     1621 ttcgacggca ctgccgcacg tgcgctccgg caagatacgg gcgctggcgg tcaccagcgc
     1681 gaagcgctcc gttgccgcgc cggagtatcc caccatcgcg gaagccggcg ttcccggcta
     1741 cagtcacgct tcgtgggtcg gcctgctcgc gcccgcgaaa acgcctcaat ccatcatcgc
     1801 cagtctcaac gccgaggcgg tcaaggccgc ccactcgcag gacgtgaagg ccgtgctgct
     1861 gcgggacggc ctcgaagcga ccggcaccac gcccggcgag tactccgcca tcatcaagtc
     1921 ggaagttgcc aagtggttca aggtggtgaa agcggccggc attgtggctc agtaatgggt
     1981 aatgagtgat gggtaatggg cgtccagtct cgagtaacag aaaaggtgaa tgaaccatga
     2041 gcctcgttga agaccttgtc gtcgcctacc gcacgctcgc gcaggaaggc gtgatcgacg
     2101 cctacggaca cgtaagcgtg cgctcggaga aggaccccaa ccgctacctg atggcgcgcc
     2161 agctcgcccc cgagctcgtg accgaggaag acatactgga gttcgacctc gacagcaatc
     2221 ccatcgaccc gcgcggcagg aagttctaca acgagcgcta catccacggc gaggtctaca
     2281 aggcgcggcc ggacgtgatg gcggtggtgc acaaccacgc gccggcggtc gtgcccttca
     2341 gctgcacttc gcgctcctcg ctcaagccga tcttccacat gtcggcgttc atcgggatgg
     2401 gcgtgcccaa ctgggacatc cgcgaagcgc agaaaggctc cgatatgctg gtgcgcacgc
     2461 cctacctcgg gcagtcgctg gcgaaaaagc tcggcaagta tccggcggtg ctgatgcgcg
     2521 ggcatggagc cacggtggtc ggcgagaacc tgcagcgcgc ggtcggccgc ggtgtctacc
     2581 tggaagtgaa
//
LOCUS       DBNGWZ010000463        26563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1068510, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000463 DBNGWZ010000000
VERSION     DBNGWZ010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 26563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1068510"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="AC16CX_14140"
     CDS             <1..1357
                     /locus_tag="AC16CX_14140"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014751353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HIR3013682.1"
                     /translation="SKEAYNTARLCLMDTLGCGLEALEYPACTKLLGPVVPGTVLPNG
                     AKVPGTSYQLDPVTAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILATADWLSRTR
                     IARGGKPLVVRDVLTAMIKAHEIQGVIALENSFNRVGLDHVVLVKVASTAVVTRMMGG
                     TREEIVNALSNAWIDGQSLRTYRHAPNAGSRKSWAAGDATSRGVRLALMALKGEMGYP
                     SALTAKTWGFYDVLFRGKPLRLKQPYGSYVMENVLFKISFPAEFHAQTAVEAAIRLHP
                     RVKDRLDAVKKIVITTHESAIRIIDKKGPLHNPADRDHCIQYMTAVGLIKGNLTAADY
                     EDAAASDPRIDRLRSKMACVENRQWSRDYLNPAKRSIANGVQVFFGDGSATDKIAVEY
                     PVGHRRRRKEGVPLLEAKFRANLARRLAPKQQQAIIALCGNPRRLEAAPVSEFVDLFV
                     I"
     gene            1363..4053
                     /gene="acnA"
                     /locus_tag="AC16CX_14145"
     CDS             1363..4053
                     /gene="acnA"
                     /locus_tag="AC16CX_14145"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3013683.1"
                     /translation="MPRDPFNALREFTPASGRTGKYYSLARLEAAGLGKVSRLPVSLR
                     IVLESLLRNCDGRRVTEEHVRNLAAWQPNGRRTAEIPFVVARILLQDLTGFLTLNDFA
                     GMRATARRLGSDPTRIEPKVPVDIVVDHQVEIDVRNSPDALRRNMELEYRRNGERITL
                     LKWAKQAFAGIRVVPPGNGIVHQVNLEYLARGVWEKDGIWYPDTLVGSDSHTPMVNGI
                     GVLGWGVGGIEAEAGMLGEPICFLTPDVVGVHMTGRLLEGVTATDLVLTVTELLRKSK
                     VVDKFVEFFGEGAASLSPTDRATVANMAPDYGATVGFFPVDEKTIDYFRVTGRDPDLV
                     DAIEGYNRAQGLFGIPRKGEIDYSQVVELDLSMIVPSVSGPRLPQQRLDLGAIKARFS
                     ELFSKPAAEAGYGKAAAELGRRYATLRAGMDVGHGDILIAAITSCTNTSNPHLLLGAG
                     LLAKKAVEKGLKVNPRVKTSLAPGSKVVTAYLRDAGLLPYLDQLGYGVVAYGCTTCMG
                     NSGPLDPAIEEAVVNNDLIGCAVLSGNRNFEARIHNNIKANFLMSPPLVVAFAIAGTV
                     DIDMTREPLGTGADGKAVHLRDIWPSDVEINAVLKFANNARNFRREYGDLMVAKDLWD
                     AIPERRGALYEWDPASSYIVEPPFFDGFTMKPGKVSNVTGARALAVFGDSLTTEHISP
                     VAAITPGSPAGRWLQSKGVKVADLTSFGARRCNHEVMVRGTFANLRIRNLMAPGKEGG
                     YTVHQSGGEVMGIYDAAERYRNEAVPLVVLAGEEYGTGSSRDWAARGPQLMGVKAVIA
                     RGFERIHRSNLVGMGILPCQFKGTDSVQSLGLDGTEGYDILGLDDSISPNQDVTLVVH
                     RKDGGIREVPLTLRVDTEIEVEYLRHGGIMQYVLRGLLAA"
     gene            complement(4076..4312)
                     /locus_tag="AC16CX_14150"
     CDS             complement(4076..4312)
                     /locus_tag="AC16CX_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013684.1"
                     /translation="MDYILDRLVICGGVNSVVKQLLAFREAVGDFGTLLYCGHDWADA
                     KLARRSMELMAEKVMPAINAAISDQPSAIRKSRR"
     gene            4556..5626
                     /gene="pdhA"
                     /locus_tag="AC16CX_14155"
     CDS             4556..5626
                     /gene="pdhA"
                     /locus_tag="AC16CX_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR3013685.1"
                     /translation="MTVIASFEIRYSQFLDPHGKAVQSLPEFARDPKNLVPLYRWMVL
                     MRSYDAKAIALQRTGQLGTFASLVGHEAINAGVGSAMAKDDVFLMTYRENGVQLMRGV
                     TLKELFLYWGGDERGSDFSGPRRDFPICITIAAHVTHAVGVAYAMKLKREQRAAVCAL
                     GDGATSKGDFYEGINAAGAWKLPVVFVVTNNQWAISVPRAKQSAAQTLAQKAIAAGFE
                     GMQVDGNDVIAVRHAMDAALAKARSGGGPTMIEALTYRLADHTTADDATRYRSPDEVA
                     EAWKLEPILRLRTYLMASGAWDKTKEDALLRDCNEQVQAAAQAYLDTPPPAAGQMFDH
                     LYATLPAALQKQRAEALEKNES"
     gene            5709..6689
                     /locus_tag="AC16CX_14160"
     CDS             5709..6689
                     /locus_tag="AC16CX_14160"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3013686.1"
                     /translation="MAQITLVEAINIALKHEMERDPDVVVLGQDVGVDGGVFRATLGL
                     TERFGAERVIDTPLAEALITGMTIGMAVEGLKPVAEIQFAGFAYTTIDQLINHAGRMR
                     HRTQGRLSCPMVLRTPCGAGIHAPEHHSESPEAMFAHIPGIRVVYPSSPRRAYGLLLA
                     AIRDPDPVVFLEPTRLYRLFKEEVADDGQVAPLDACYTAREGSDITLVAWGAMVQEAL
                     AAADDLVEQGVAAEVIDVSTLKPLDMDTILASVAKTGRCVIITEAAHTCGFASEIAAS
                     LAEDGLLTLLAPVRRVTGYDVVVPLPRLERQNLPDKERIIAAARKALAFA"
     gene            6832..7935
                     /locus_tag="AC16CX_14165"
     CDS             6832..7935
                     /locus_tag="AC16CX_14165"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3013687.1"
                     /translation="MKIFKLPDLGEGLQEAEIVKWHVEVGDEVKADVPIVSVETAKAV
                     VDIPAPYSGRVAKLYGKPGDIAHIGAPLIAFEGESDAPDTGTVVGKVEVGQKVVADTP
                     AAPVPVTGGIKATPAVRALARKLNVDLAMVTPTGADGLLTVHDVQRAARILAETGPIE
                     MMRGVRRAMAQNMELATAEVASTTIMDDADIEAWPDGTHTMLRLIRALVAGCRAEPSL
                     NAWYDSEKIGRWVLKKIDIGIAVDTPDGLLVPVLRNCGERSPEDLREALAKLIVDARA
                     RKVPPEELRANTITLSNYGSIAGRYASPVVLPPTVAILGAGRIQKQVVVMDDKPAVRR
                     TLPLSLTFDHRAVTGGEAGRFLNAVIADLQLAK"
     gene            complement(8037..9077)
                     /locus_tag="AC16CX_14170"
     CDS             complement(8037..9077)
                     /locus_tag="AC16CX_14170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3013688.1"
                     /translation="MLHFPELNRGKCKTYLVACARTKKALLIDPVRDHIGRYLGFLAY
                     QRLTLDAVVDTHTHADHPTGSFQLRDLVGARLIMHRRAPAPIVTHHVEDGDDIELGES
                     RLRVLYTPGHTPDSISLYGDGRVFTGDVLLIGGTGRADFAGGDAGQQYDSITNKLFTL
                     PDETLVFPAHDYRGNTSSTIAKEKRTNPRIAGRARAQYIELMASLNFPMPDKIQEVLQ
                     PNQSAIDDDRARFPDLAELNRVRQLAAEEVIALLASASPPLVLDVREESEYHGDLGHI
                     PASILIPLRELAARVTEIETYRGRQIVAVCRSGVRSTTAAAMLYGLGFERVYNLKDGM
                     VEWNDRKCPVER"
     gene            complement(9237..10472)
                     /locus_tag="AC16CX_14175"
     CDS             complement(9237..10472)
                     /locus_tag="AC16CX_14175"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR3013689.1"
                     /translation="MAKQPWKTDNWFVSQWNFADEVRKDFKFAKQIKIHDITLRDGEQ
                     QTGIILSKDDKIRIAEALAEAGVHRIEAGMPIVSPSDAEAIKEIARRNLGPQIFAFSR
                     CMKEDVQRAIDTGVNGVVMEIPSSQHMVDLAYKWPMEKAIETSIEATKFAQKNGLEVV
                     FFPIDFSRSEMNWVLNLINRVATEGHMDALALVDTFGVVSPHAMRYFVREVKKRINKR
                     LEAHFHQDFGMGVANTIMAAAEGVEVLHTTVLGVGERAGNTPMEETVMALLTMYGVDV
                     GLDYSKLTPLANLVQKLTGVDVPSNKPVVGRQLYQIESGIIASWYKNCGEENATELFP
                     VRAKFVGQPPAEIVMGKGSGIDSVKAWLAQMGIEASDDEALKMTAAVKGYSLKNKRLL
                     TEAEFRKISQDVLGQRAAA"
     gene            complement(10711..11214)
                     /locus_tag="AC16CX_14180"
     CDS             complement(10711..11214)
                     /locus_tag="AC16CX_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013690.1"
                     /translation="MQSARRICLLAVAAGAAITVPAFAATPDPTPSDDVLMVQAAPDA
                     IHFRPDSGHAKYSWLVGGEWQRPSRWLAGYAYFNNSFDQKSHYIYGGRWWPLHEGDPG
                     WYFKLTGGLILGYKGPHEDKIPFNHNGVAPGLIPGLGFKAGRANVQFNLLGTAGMMIT
                     FGYDLPR"
     gene            complement(11257..12144)
                     /locus_tag="AC16CX_14185"
     CDS             complement(11257..12144)
                     /locus_tag="AC16CX_14185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3013691.1"
                     /translation="MIPAKRLRYSAIVDRPPLVLPDRARVVVWPVVNVEVWDVGRSMP
                     RQVLPPPTGVTQLPDVANWGWHEYGMRVGFWRLKAALDGLGIVPSLSINARVCLDYPK
                     VAKAARDGGWEFMGHSYDQRPMHGEPDPGEMVRRSVRVIREFTGKRPVGWMGPGLTEQ
                     LDTPELLAAAGIRYIADWVIDDEPCMIKTRNGSLVTMPYTLELNDIAMMVVQHHAARE
                     WETRCMDYFDRVYREGRERAKIMAIAVHPYISGVPHRIKYFETVLRRLKKQKGVLFWT
                     GGEILKWYLAARGRPAAGR"
     gene            complement(12362..12820)
                     /locus_tag="AC16CX_14190"
     CDS             complement(12362..12820)
                     /locus_tag="AC16CX_14190"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3013692.1"
                     /translation="MFLASVIMICSGWAVVAAWDWPWKAKLFPLVIGIPVFCLATAEV
                     LWVMFGTTRQDQAMDFQLSRDLPARVAFWRTLRAAGWMLGFFGAIVLVGFPIAVPLFV
                     LLYLKVEGREGWGLSLLITLAAWAFFYALFDRLLHVPFPAGWIQSWAGLG"
     gene            complement(12831..14342)
                     /locus_tag="AC16CX_14195"
     CDS             complement(12831..14342)
                     /locus_tag="AC16CX_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3013693.1"
                     /translation="MIDALFEGLLLVLQWKAFAFMLVGIGLGFWVGLLPGLGGATTLA
                     LMLPFVYAMEPVEGFAFLLGMHSVVATTGDITSVLFGVPGEATTAATILDGHAMAKKG
                     EAGRALGAVLASSLIGALVGALALALSVPVVRPLVLSFGSPELFMLAIVGLAFIASLS
                     SQGGRGMLRGFLAGGVGLMLATVGQDPQAGIARYTFDSLYLWGGIDLVPVLIGIFAIP
                     EIIDLAVRGSSIAGDVPSGRLSKGALEGVKDTFRHFWLTIRCSLIGTFIGIMPGLGGA
                     VAQWVAYGHATQSARNAEERKQFGRGDVRGVLGPGAANNSKEGGSLIPTVAFGVPGSS
                     AMAILLGGFFLMGLVPGPDMLTKHLVVTFSMVWTIVLANIITVGACFLFLNHLANLTA
                     VRGHLLIPLILLLVFIGSYTSNNHYGDLLVTLLFGGIGYLMVMAGWPRAPFVLGLVLG
                     KIAENYLYISVARYETAWLYRPIVLVLFALAIAVILYPLLQARRSSLKKETHA"
     gene            complement(14339..15349)
                     /locus_tag="AC16CX_14200"
     CDS             complement(14339..15349)
                     /locus_tag="AC16CX_14200"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013694.1"
                     /translation="MRSTLALIAVFTLSALAPRVGEAQTSPFEGKTITIVVGFKTGGG
                     YDRAARILARHLPKHVPGKPNVIVQNMPGANSIISANHVYSVARPDGLTIGTFNRNLV
                     LAQLTSVSGVKYDMRKFAWIGSAASETTILTIRSDLPYKSFDELRKAGKTVVIGATGP
                     GANTYDFPLLLKELLGVDLKIVTGYSSSSDIMLAVERKEVDGRAGSYSSLKPFIDRNL
                     VRPLVRARAIEAGIEQLPVDENLAPNPRAKAIMALRSAPEVIGRPYVMPPGTPDNVVR
                     MMREAFRNAIKDPELAAEATKAKMEFEYVSADETLKVLNEVLSQPKDIVDAFSKYIKF
                     GE"
     gene            complement(15476..16528)
                     /locus_tag="AC16CX_14205"
     CDS             complement(15476..16528)
                     /locus_tag="AC16CX_14205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3013695.1"
                     /translation="MGKRIVFIGAGALGGYVGGHLTRAGHDVTLIDPWPEHVEYMRRN
                     GIQLSGTTPAERYSVPVNARHVCDVQGLVKQKPIDIAFVSVKSYDTEWATLLIRPYLA
                     PDGFVVSLQNCINEERIAQHIGWGRTVGCIASLIAVELYQAGHVKRTVPMGGERHTVF
                     RVGEPHGRVTRRVEEVAELLRSVDSAKVTTNLWGERWSKLVVNSMRNGLSAATGLYGN
                     QRDALEGPRWVSIRLGSEAVRVGQTLGFQLERMQGMEPEALARAGEGHRDALAEITDI
                     IVEDATRRSDDQRPSMAQDIAKGRRTETDFINGFVAARGAEIGVPAPMHAKMNEAVKR
                     VERGEVKPSPELIAGW"
     gene            complement(16625..17677)
                     /locus_tag="AC16CX_14210"
     CDS             complement(16625..17677)
                     /locus_tag="AC16CX_14210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012407.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3013696.1"
                     /translation="MRAITLIPGDGIGPEVTAAARAVVLASGAPVQFEEVVAGQRAER
                     EHGTPLPRSVFDSIDRTRLALKGPTQTPFGGDYRVNIERADASGKIGKRTYPSVAIAL
                     RKELGLFVNVRPVKNYSGVASRFEHVDLLIFRENSEDLYVGMERMVDEGTAEAVKIIT
                     ARGTERVTRFALDAMRRLGRRRVTIVHKANVLKMTDGLFLKTAQDVARGSGFTVDQRV
                     VDALCMELVMKPEAFDCLLLPNLYGDIVSDLAAGLVGGLGVAPGSNIGAECAMFEAVH
                     GSAPDIAGKDIANPMAMILSAVMLLRYIGETVTADRIETALVQALRERRTVTRDLGGT
                     AGTRAMADAIIEKLKS"
     gene            complement(17759..19270)
                     /locus_tag="AC16CX_14215"
     CDS             complement(17759..19270)
                     /locus_tag="AC16CX_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR3013697.1"
                     /translation="MDTLAHILNGFSVCLQPVNLWYTFLGCFLGTIIGVLPGIGPSAT
                     IALLIPVTYGMNPTSALIMMSGIFYGARYGGSTTAILINTPGEASSVMTTLDGYQMAK
                     KGRAGAALAISALGSFFAGTLGVVGLTLFAVPLASMALKFGPAEYFTLMFFAMTAVSS
                     LAGKSPAKAMISTILGLMIATIGIDLQSGQPRYTMGVPELQDGVGFIIAVVGLFAIGE
                     VFSTMEDMTKGIRPEIIRLSGRLWLTREEWNRSVMPMLRGGIIGFIIGVLPGAGGTIA
                     AILSYVWEKRLSKHPEQFGTGAIEGVAGPEAANNSDTAGAMVPLLTLGIPGGGATAVM
                     LGAFIMYGIQPGPLLFQNRPDLVWGLIDSMYIGNIMLLILNLPLIGLFVRLLYIPSGI
                     LMALIMAISTIGIYAINGNPVELYIGLVFGVMGYVFRKVDIPVAPMVLSLVLGGIMEQ
                     SFRQAMTISGSNPKIFVGSVICVTLVALSILSILLPFLLPRLKAWREGSSDTA"
     gene            complement(19270..19734)
                     /locus_tag="AC16CX_14220"
     CDS             complement(19270..19734)
                     /locus_tag="AC16CX_14220"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3013698.1"
                     /translation="MMTDRVIFVCTIIVAAVYLYATTLIPSLEIGDPLGPKAFPRLLG
                     IGLLLGAGLLFVEMWKDRKARTSQSASAEAAQWRYAGVIAAVTVWTGIYYAMFEKLGY
                     IVATAIYLFALMAWFNRGKWPANALTSVLFCVLSYWMFVKLDVNLPKGILPF"
     gene            complement(19820..21433)
                     /locus_tag="AC16CX_14225"
     CDS             complement(19820..21433)
                     /locus_tag="AC16CX_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013699.1"
                     /translation="MPKTADILARALKEHGVRHAFGIPGGEVLELLEAFRKAGIRFIL
                     TKQELGAGFMADAAFQLTGKPGVLVATLGPGLTNTVTAVAQALLDRSAMIVITGEIAA
                     ALRSIYTHQIIDQCGILGPLTKWSATFAARGALEQARKGIAIALTPMPGPVHFNVPTD
                     VAAMEQETGGRFATASIAGQPPAQDLSRAIGWLRTARRPLALVGVGVQLDSGAKELRY
                     FLERWRVPVVTTYKAKGVIPEDHPLCIGGAGLSPVVDAIHMARIRDADLVLCIGFDPV
                     ELRSDWIAPWDRSKRTMSFDLVPNTHHVYRCGLEYAGAITAALGQLAAAAPAKPPRRW
                     PDGELQRYREAIRESVAHQPRRGLGPYQATTTLREVFPRETIATVDTGSHRILINHVW
                     RSYEPRRLLQSNGLGSMGYALPAAIAAKLLHPKRPVLAMTGEAGLDMVIGEMALLAHH
                     RLAVTIVVFRDDTLSLIKLKQERMKLPETGVATGSPDYATLAKAYGGNGLVAESVAEL
                     KKAARSALASRRFTLIEARIDPAEYRKQM"
     gene            21787..24471
                     /gene="acnA"
                     /locus_tag="AC16CX_14230"
     CDS             21787..24471
                     /gene="acnA"
                     /locus_tag="AC16CX_14230"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3013700.1"
                     /translation="MSHNLFNSLQEFKYASKTGKYYSLPALEKAGLGKISRLPISLRV
                     VLESALRNCDGKKITEAHLKNMAGWKPKAKRTEEVPLVVARVLLQDMTGVPLVVDFAA
                     MREAAKVMGKNPEIIEPLCPGHLIMDHSVQIDNYGTPDALRKNMEIEFKRNRERYQFL
                     KWSASAFKTFRIIPPGNGICHQINLEYVSQVVWQKDGMFYPDSLVGIDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPMYFLEPDVVGVHLTGKLNDGVTATDLVLTIVKLLRDTKV
                     VGKFVEYFGEGAASLPVPDRATIGNMSPEYGATCGFFPVDDVSCDFLKMTGRGNIVPA
                     VKAYWKAQGMYGMPKKGDIDYSQIVELDLSTVKPAVSGPRRPHDHLDLPKIGAKYREL
                     MGKPFTDGGYGKPNELGKRVATSRSGVDVGHGDVLISAITSCTNTSNPGVLLGAGLLA
                     KKAVEKGLKPHPRVKTSLGPGSLAVTEYLKNAGLLPYLEQLGYYVAGYGCTTCIGNSG
                     PLDAHIDEAVAKNDIVAAAVLSGNRNFEARIHQNIRANFLMSPPLVVAFGLAGTIDID
                     MSKDPIGTGKDGKPVYLKDIWPSTREVGAAMKYATDPEMYRKLFSAFGEGNPMWDEIP
                     ASVGATYQWDEKSTYVRLPPYFEGFSMKPGKVTDIRGARVLGIFGDAITTDHISPAGG
                     IRPTAPAGKYLQDHGVKIPDFNTYGARRGNHEVMMRGTFANIRIKNLMVPGTEGGITI
                     HHPSGEQTSIYEAAMRYKKEGVPSVVFGGDEYGQGSSRDWAAKGPQLLGVKACIVRNF
                     ERIHRANLVFMGVLPLEFKDGATAQSLKLDGSETIDLLGIEKGITPMQDVTMVIHRKN
                     GDATKVPLKLRIDTPIEVDYYQHGGILPYVLRQLIAAA"
     gene            24572..26014
                     /locus_tag="AC16CX_14235"
     CDS             24572..26014
                     /locus_tag="AC16CX_14235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011325457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3013701.1"
                     /translation="MASSARSAVPASLLERVGSIVGPQGLITDLRDIEPHIVDWRGLY
                     RGATPAVVRPASSAEVAEVVKACAETRTAVVPQGGNTGMCGASVPRAEGGEIVLNLAR
                     MNRILEVDALNNAVSVQAGCILANIQQAARDAGRLFPLSLGAEGSCQIGGNLSTNAGG
                     VNVLRYGNARDLALGLEVVLPDGRLWDGMRSLRKDNTGYDLKQVFIGAEGTLGIITAA
                     VLKLFPLPHSGATAWAAVSSPEAALKLLALLRQQCGDKVTAYELISRNCLDLVLRHIP
                     GTRDPLAGRHAWYVLADLSDERESGAIRGELEQVLAAAIERGLASDAVIAESQSQAQG
                     LWRLREAIPEAARAEPGMLYRHDIAVAVSRVPAFIAEAQAALQLRFPGVDVICFGHLG
                     DGNLHYNAFVPGRRREDASAREARDVTQMVYDIVSRYHGSFSAEHGIGQYKLAEIIRY
                     KGGVELEMMHALKRAFDPHNIMNPGKVLPE"
     gene            26040..26435
                     /gene="msrB"
                     /locus_tag="AC16CX_14240"
     CDS             26040..26435
                     /gene="msrB"
                     /locus_tag="AC16CX_14240"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3013702.1"
                     /translation="MTAKIEKSDAEWRKQLTPEQFHVAREKGTERAFSGAYWNHHEPG
                     VYRCVCCGVELFSSEHKFDSGTGWPSFWQPVGRENVATEDDRSFFMHRTEVLCGRCDA
                     HLGHVFDDGPQPTGLRYCINSASLKFERR"
BASE COUNT         4938 a         8728 c         8309 g         4588 t
ORIGIN      
        1 gagcaaggag gcctataaca cggcgcggct gtgcctgatg gacacgctcg gctgcgggct
       61 ggaagcgctc gagtaccccg cctgcaccaa gctgctcggc cccgtcgtcc ccggcacagt
      121 gctgccgaac ggcgcgaaag tgcctggcac gtcctaccag ctcgacccgg tgaccgcagc
      181 tttcaacatc ggcgcgatga tccgctggct cgatttcaac gacacctggc tcgccgccga
      241 gtggggccac ccctccgaca acctgggcgg catcctcgcc accgccgact ggctctcgcg
      301 cacccgcatc gcccgtggcg ggaaacccct cgtcgtgcgc gacgtgctca ccgccatgat
      361 caaggcgcac gagatccagg gcgtgatcgc gctcgagaac agcttcaacc gcgtgggcct
      421 tgaccacgtg gtcctggtga aagtcgcctc caccgccgtc gtcacccgca tgatgggcgg
      481 cacgcgcgaa gaaatcgtca acgcgctgtc gaatgcgtgg atcgacgggc agagcctgcg
      541 cacctatcgc cacgcgccca acgccggctc gcgcaagtcg tgggcggcgg gcgacgccac
      601 cagccgcggc gtgcggcttg ccctcatggc gctgaagggc gagatgggct acccttcggc
      661 gctcaccgcg aagacctggg gcttctacga cgtgctgttc cgcgggaagc ccctccgcct
      721 caagcagccc tacggttcgt acgtgatgga aaacgtgctg ttcaagattt cgtttcccgc
      781 ggaatttcac gctcagaccg cggtagaggc cgcgatccgt ctgcatcccc gggtaaagga
      841 ccgcctggac gcggtgaaaa agatcgtcat cacgactcac gaatccgcga tccgcatcat
      901 cgacaagaag ggccctcttc acaatcccgc ggaccgcgac cactgcatcc agtatatgac
      961 ggcggtcggg ctcatcaagg gcaacctgac cgcggccgac tacgaggacg ctgccgcctc
     1021 cgatccgcgc atcgaccggc tgcgatcgaa aatggcgtgc gtcgagaaca ggcaatggag
     1081 tcgcgactat ctgaaccccg cgaagcgctc catcgccaac ggggtgcagg tgttcttcgg
     1141 cgacggctcg gcaaccgaca agatcgcggt cgaataccct gtcggccatc gccgccgccg
     1201 caaggaaggt gtgccgttgc tcgaggcgaa gttccgggcc aatctcgccc gccgcctggc
     1261 gcccaagcag cagcaagcca tcatcgcgct gtgcggcaac ccgcggcggc tcgaagcggc
     1321 acccgtcagc gagttcgttg acttgttcgt gatttgactg tcatgccacg cgatccattc
     1381 aacgccctcc gcgaattcac tcccgcctcg gggcggaccg gaaaatatta ctcactcgcc
     1441 aggctggagg cagcgggact cggcaaggtc tcgcgcctgc ccgtgagctt gcgtatcgtg
     1501 ctcgagtccc tgctcaggaa ctgcgacggc aggcgcgtga ccgaggaaca cgtcagaaac
     1561 ctggccgctt ggcaaccgaa cggccggcgc actgcggaga tccccttcgt cgtggcgcgc
     1621 atcctgctgc aggacctgac cggttttctc acgctcaacg atttcgcggg gatgcgcgcc
     1681 accgcccgcc gcctcgggtc ggaccctacg cgcatcgagc cgaaagtgcc cgttgacatc
     1741 gtggtggacc accaggtcga gatcgacgtc cggaattccc cggacgcgct ccggcgcaac
     1801 atggagctcg agtaccggcg caacggcgag cgcatcacgc tcctcaagtg ggcgaagcag
     1861 gcgttcgccg gcatccgcgt cgtgccgccg ggcaacggca tcgtgcacca ggtcaacttg
     1921 gaatacctgg cgcgcggcgt gtgggagaag gatggcatct ggtatcccga caccctggtc
     1981 ggttccgact cgcacacccc catggtcaac ggcatcggcg tgctcggctg gggtgtgggc
     2041 gggatcgagg cggaagccgg gatgctgggc gagccgatct gcttcctcac gcccgacgta
     2101 gtgggcgtgc acatgaccgg ccggttgctc gagggcgtca ccgccaccga tctcgtgctt
     2161 accgtcaccg agctgctgcg aaaatcgaaa gtggtcgaca agttcgtcga gtttttcggc
     2221 gaaggcgccg cctcgctctc ccccaccgac cgcgcgacgg tcgccaacat ggcaccggac
     2281 tacggcgcca ccgtcggctt ttttccggtg gacgagaaaa ccatcgatta tttccgtgtc
     2341 accgggcgcg atcccgacct cgtagacgcg atcgagggct acaacagggc ccaaggcctg
     2401 ttcgggatcc cgcgcaaagg cgaaatcgac tactcccagg tcgtcgagct ggatctctcg
     2461 atgatcgtgc cgagcgtctc cgggccaagg ctgccgcagc agcgcctcga cctcggggcc
     2521 atcaaggcgc gtttttcgga gcttttctcg aagcccgccg cggaagccgg gtacggcaag
     2581 gcggccgcgg aactcggcag acgctacgca accttgcgcg cgggcatgga tgtcggccac
     2641 ggcgacatat tgatcgccgc catcacgtcc tgcaccaaca cttccaaccc gcacctgctt
     2701 ctcggcgccg ggctgctggc gaaaaaggcc gtcgagaagg ggctgaaagt gaatccgcgt
     2761 gtgaagactt cgcttgcgcc cggatccaag gtcgtcaccg cctatctgcg cgacgccggg
     2821 ctgctgccgt acctggatca gcttggctat ggcgtggttg cctacggctg taccacctgc
     2881 atgggcaact cgggaccgct cgatcccgcg atcgaggaag ccgtcgtgaa caacgacctc
     2941 atcgggtgcg cggtgctctc gggcaatcgc aacttcgagg ctcgcatcca caacaacatc
     3001 aaggcgaatt tcctgatgag cccgccgctg gtggtggcct tcgcgatcgc cggcaccgtg
     3061 gatatcgaca tgacgcgcga gcccctcggc accggcgccg acgggaaggc ggtccacctc
     3121 cgtgacatct ggccgagcga cgtcgaaatc aacgcagtgc tcaagttcgc gaacaacgcg
     3181 cggaacttcc gccgcgagta cggcgacctg atggtggcga aagacctgtg ggacgcgatt
     3241 cccgagcgga ggggcgcgct ctacgaatgg gaccccgcgt ccagctacat cgtcgagccg
     3301 ccgttcttcg acggatttac catgaaaccc gggaaggtct cgaacgtcac cggggcccgc
     3361 gcacttgccg tattcggtga ttcgctgacc accgaacaca tcagcccggt ggcggcgatc
     3421 acgcccggct cgccggcggg ccgctggctc cagagcaagg gcgtcaaggt tgccgatctg
     3481 accagcttcg gggcgcgccg ctgcaaccac gaagtcatgg tacgcgggac tttcgccaac
     3541 ctgcgcatca ggaacctgat ggcccccggc aaggaaggcg gctacaccgt gcatcagtcc
     3601 ggcggcgaag tcatgggcat atacgacgct gcggaacgct accggaatga agccgtgccg
     3661 ctggtggtgc tcgccggcga ggaatacggc accggctctt cgcgcgactg ggccgcgcgc
     3721 gggccccaac tgatgggcgt gaaagccgtg attgcccgcg gcttcgagcg catccaccgc
     3781 tccaacctgg tcggcatggg catcctgccc tgccagttca aaggaaccga ctccgtgcaa
     3841 tcgctcggcc tggacgggac cgaaggttac gacatcctgg gcctggacga cagcatcagc
     3901 ccgaatcaag atgtgaccct ggtagtccat cgcaaggacg gcggcatccg ggaagtgccg
     3961 ctcaccctgc gcgtggacac tgagatcgag gttgaatacc tcaggcacgg cggaatcatg
     4021 cagtacgtgc tacgcggctt actggccgca tagcacgtca gccgtcagca tacagctatc
     4081 ggcgcgattt tctgatcgct gacggctgat cgctgatcgc cgcattaatg gcgggcatca
     4141 ccttctccgc cattagctcc atcgagcgcc gcgcgagctt cgcatcggcc cagtcgtggc
     4201 cacagtagag cagcgtgccg aaatcgccga ccgcctcgcg gaaggcaagc agctgtttca
     4261 ccacgctgtt gacgccacca cagatcacca gccggtcgag gatgtaatcc accgtcaccg
     4321 cggcgaccgg tcgcggattt cgttccagcc aagcggcatt gcgccgtccc tctccccgta
     4381 acgctccgag cctaagttgt agtctaatag ctgtcaaatg cgtcggcgta ccaggacggc
     4441 gcaaagtgcg ataatctccc ccggctccgc gcagacgagc cagaggaaag aggatgaaag
     4501 cacaataccc gccctctcga atcacgactc accactcacg attcacggcc gtcaagtgac
     4561 cgtcatcgcc tccttcgaga tccgctattc gcaattcctc gacccgcacg gcaaggccgt
     4621 gcaatcgctg ccggaattcg cgcgcgatcc gaagaacctc gtccccctct atcgctggat
     4681 ggtgctgatg cgctcctacg acgccaaggc gatcgcgctg cagcgcaccg gccagctcgg
     4741 caccttcgcc tcgctggtgg ggcacgaagc catcaacgcc ggcgtcggtt ccgcgatggc
     4801 aaaggacgac gtgttcctca tgacctaccg cgagaacggc gtgcagttga tgcgcggcgt
     4861 cacgctgaag gagctgtttc tatactgggg tggagacgag cgcggctcgg acttttccgg
     4921 accgcgccgg gattttccga tctgcatcac cattgccgcg catgtcacgc acgcggtggg
     4981 tgtcgcctac gccatgaagc tgaagcgcga gcagcgcgcc gcggtctgcg cgctcgggga
     5041 cggcgcaacc tccaagggcg atttctacga gggcatcaac gcagcaggcg cttggaagct
     5101 gccggtggtg ttcgtcgtca ccaacaatca gtgggcgatc tcggtaccgc gcgcgaagca
     5161 gagtgcggcg caaacgctag cccaaaaagc gatcgccgcc ggcttcgaag gcatgcaggt
     5221 ggacggcaac gacgtcatcg ccgtgcggca cgccatggat gccgcacttg ccaaggcgcg
     5281 ctcgggcggc ggccccacga tgatcgaagc cctcacctac cggctggcgg accacacgac
     5341 agcggacgat gccacgcgct accgcagtcc cgacgaggtc gccgaagcat ggaagctcga
     5401 gcccattctg cggctgcgga cctatctcat ggcaagcggc gcgtgggaca agacgaaaga
     5461 ggacgcgctg ctcagggatt gcaacgagca ggtgcaggcg gcggcacaag cgtatctcga
     5521 tacgcccccg cctgctgcag ggcagatgtt cgatcatctc tacgcgacgc ttccggcagc
     5581 gctgcagaag cagcgtgcgg aagcgctcga aaagaatgag tcgtgaatca aaaaaataca
     5641 ttgactcaga aacctatcac ttatcaccta tcacttatca cctatcactc atcacgcagt
     5701 aataggcaat ggcccagata acgctcgttg aagcgatcaa catcgccctc aagcacgaaa
     5761 tggagcgcga ccccgacgtc gtggtcctcg gtcaggacgt gggcgtggac ggcggcgtgt
     5821 ttcgcgcgac gctggggctc accgagcgtt ttggagccga gcgtgtcata gacaccccgc
     5881 tcgccgaggc gctgatcacc ggcatgacga tcgggatggc cgtcgaaggc ttgaagccgg
     5941 tcgccgagat ccagttcgcc ggcttcgcct acaccaccat agaccagctc atcaatcacg
     6001 ccgggcgcat gcgtcatcgc acccagggcc ggctctcctg cccgatggtg ttgaggacgc
     6061 cctgcggggc cggcattcac gcgcccgagc accactcgga gagtccggag gcgatgttcg
     6121 cacacatacc cggcatacgg gtcgtctatc cctcctcacc gcgccgcgcc tacggactgt
     6181 tgcttgccgc gatccgcgat ccggacccgg tggtgttcct cgagccgacg cggctctacc
     6241 gcctgttcaa ggaggaagtc gcggacgacg gccaggtcgc gcccctggat gcctgctaca
     6301 cggcgcgcga gggcagcgac atcacgctgg tggcatgggg cgcgatggtc caggaggcgc
     6361 ttgccgccgc cgatgacctc gtcgagcagg gggttgcagc cgaagtgatc gacgtttcca
     6421 cgctcaagcc gctcgacatg gacaccattc tcgcttcggt cgcgaagacg ggccgctgcg
     6481 tgatcatcac tgaagccgcg cacacttgcg gcttcgcctc agagatcgcg gcgagtcttg
     6541 cggaggacgg gctgctcacg ctgctcgcgc ccgtgcggcg cgtaaccggt tacgatgtcg
     6601 tcgtgccgct gccgcgcctc gagcggcaaa acctgccgga caaggagcgc attatcgctg
     6661 cggcgagaaa ggcgctcgcc ttcgcctgac accggtgact gagtgactaa gtgattgaat
     6721 gattaagtaa gtgcttttag cctggatcca aacgttactt gcgatctgcc gccagccgct
     6781 cagtcgctta atcacttaat cacctaatca cttaatcact gctttcatac catgaagatc
     6841 ttcaaattgc ccgatctggg tgaaggcctg caagaggccg aaatcgtcaa gtggcacgtc
     6901 gaggtcggcg acgaagtgaa ggctgacgtg ccgatcgtgt cggtggagac ggcaaaggca
     6961 gtggtggaca ttcccgcgcc ttattcaggc agggtcgcca aactctacgg caagccgggg
     7021 gacatcgcgc acatcggcgc accgctcatc gcgttcgagg gcgagagcga cgcgccggac
     7081 accggcaccg tcgtcggcaa ggtcgaggtc ggtcagaaag tagtcgccga tactccggct
     7141 gcccccgtgc cggtcaccgg cgggatcaag gccacgcccg cggtgcgcgc gctcgcgcgc
     7201 aagctcaacg tggacctcgc catggtcacg ccaacgggag ccgacggcct gctcaccgtc
     7261 cacgacgtgc agcgcgcggc gcgcatcctg gccgaaaccg gaccgatcga gatgatgcgc
     7321 ggggtgcggc gtgcgatggc gcagaacatg gaactcgcca ccgccgaggt cgcttccacc
     7381 acgatcatgg acgacgccga catcgaggcc tggcccgatg gcacccacac catgctgcgg
     7441 ctgattcgcg cgctcgtcgc cggctgtcgc gccgagccct cgctcaacgc ctggtacgac
     7501 agcgagaaga tcggccgctg ggtgctgaag aaaatcgaca tcggcatcgc ggtggacacg
     7561 cccgacggat tgctggtgcc ggtgctgcgc aattgcggcg agcgcagccc ggaggatctc
     7621 agggaagcgc ttgcgaagct gattgtcgac gcccgcgcgc gcaaggtgcc ccccgaggag
     7681 ctgcgcgcca ataccatcac actttccaac tacggctcga tcgccgggcg ctatgcttct
     7741 cccgtagtgc tcccccccac ggtagcgatc ctcggcgcgg gccgcatcca aaagcaggtc
     7801 gtggtgatgg acgacaagcc cgctgtgcgc cgcacattgc cgctctcgct cacgttcgat
     7861 caccgcgcag taaccggcgg cgaagcagga cgttttctaa acgccgtgat cgcggatctg
     7921 cagttggcaa aatgatcgtg agcggtaagc ggtaagaaat ttgcccccct ctgttggaag
     7981 ttccccgctc tctcaccacc actcactgct tactgctcac cgctcgcggc ctttggtcag
     8041 cgctcaaccg ggcatttccg gtcgttccat tcaaccatgc catccttgag gttatagacg
     8101 cgctcgaagc ccaggccata gagcatggca gccgccgtcg tactgcgcac accggagcgg
     8161 cacacggcca cgatctgccg gcctcggtaa gtctcgatct cggtaacgcg tgccgccagt
     8221 tcgcgcaatg ggatcagtat gctggcggga atgtgcccta ggtcgccgtg gtactcgctt
     8281 tcctcgcgca catcgagcac cagcggtgga ctggcggatg cgagcagcgc gatcacctcc
     8341 tcagcggcaa gttgccgcac ccggttcagc tcagcgaggt cggggaacct ggcccggtcg
     8401 tcgtcgatcg cgctttgatt gggctgtagc acctcttgga tcttgtccgg catcgggaaa
     8461 ttgaggctcg ccatcagctc gatgtattgc gcgcgtgcgc gtcccgcgat gcgcggattg
     8521 gtgcgttttt ccttcgcgat ggtgctgctg gtgttgccgc ggtaatcgtg ggccgggaac
     8581 accagtgtct catcgggaag tgtgaagagc ttgttcgtga tcgagtcgta ctgctgtccg
     8641 gcatcgccgc ccgcgaaatc cgccctcccg gttcccccga tgagcaggac gtcgccggtg
     8701 aaaacgcggc cgtcaccata gaggctgatg ctgtcgggcg tgtggcccgg cgtataaagt
     8761 acccgcagcc gggactcgcc cagctcgata tcgtcgccgt cctcgacgtg atgcgtcact
     8821 atgggagccg gcgcgcgccg gtgcatgatc agccgcgcgc cgaccaggtc gcgcagttgg
     8881 aacgagcctg ttggatggtc ggcgtgcgta tgggtatcca cgaccgcgtc caacgtgagc
     8941 cgctgatagg cgaggaagcc gagataacgg ccgatgtggt cccgcaccgg gtcaatgagc
     9001 agcgccttct tcgttcgcgc gcaggcgaca aggtaggtct tgcacttgcc tcggttgagt
     9061 tccgggaaat gcagcatgcc ttcccccact tcgataatac cggacctgtg cctggttagc
     9121 ggtacaaaac aaaacggggg gatataggac gtatcccccc gttccccgcc atgccgtcag
     9181 agccgggcgt cgctaacggc gcccccagcg tgaccggctg gctgtcgcag ttcgcgttac
     9241 gcagcggcgc gctgcccgag gacgtcctgc gaaatcttgc ggaattcggc ttcggtcagc
     9301 agccgcttgt tcttcagaga ataccccttc accgcggccg tcatcttcag ggcctcatcg
     9361 tcgctcgcct cgatgcccat ctgcgcgagc caggccttga ccgaatcgat accgctgccc
     9421 ttgcccatca cgatctcggc gggtggttgc ccgacgaact tcgcgcgcac ggggaacagc
     9481 tcggttgcgt tttcctcgcc gcagttcttg taccagctcg cgatgatgcc agactcgatc
     9541 tgatacagct gcctgcccac gacgggcttg ttcgaaggga catccactcc ggtgagtttc
     9601 tgcaccaggt tggcgagcgg cgtcaacttg ctgtagtcga gcccgacgtc cacgccatac
     9661 atcgtcagca acgccatgac ggtctcttcc atcggcgtgt tgccggcgcg ctcgccgacg
     9721 ccgagcacgg tcgtgtgcag cacctcgacg ccttcggcag ccgccatgat cgtgttcgcg
     9781 acgcccatgc cgaagtcctg gtggaaatgc gcttcgaggc gcttgttgat gcgcttcttg
     9841 acctcgcgga cgaagtagcg catggcatgg ggcgacacga ccccgaaagt gtccaccagg
     9901 gcgagcgcgt ccatgtggcc ctcggtcgcg acccggttga tcaggttcaa cacccagttc
     9961 atctcggagc gggagaagtc gattgggaag aacacgactt ccagcccgtt cttctgagcg
    10021 aacttggtcg cctcgatcga ggtctcgatc gccttctcca tcggccactt gtaagcgagg
    10081 tccaccatgt gctggctgga cgggatctcc atcaccacgc cgttgacgcc ggtatcgatc
    10141 gcgcgctgga cgtcctcttt catgcaccgc gaaaaggcaa agatctgcgg gccgaggttg
    10201 cgcctggcga tctccttgat ggcttcggcg tcggagggcg agacgatcgg catacccgct
    10261 tcgatgcggt gcacgccagc ctccgccagc gcttcggcaa tgcggatctt gtcgtccttg
    10321 ctgaggatga tgccggtttg ctgctccccg tcacgcagcg tgatgtcgtg gatcttgatc
    10381 tgcttggcga acttgaaatc cttgcgtact tcgtccgcga aattccactg gctgacgaac
    10441 cagttgtctg tcttccaggg ttgttttgcc atttgatgct ccttgcgtgt tgagtgaggt
    10501 tcgttcgaac agtttcccta cgaccgcgtg gtcaactcat tgcgtatggg gaaatttccc
    10561 ttggctgctc gcagagaccg cgtggttaaa accggcgctt tcccggtagc ccggtatgag
    10621 aggctaacat gttagttttc aacaaaaatc gcgctgctgt caaggcaagc cgggaaagca
    10681 tgacgtcgac gtttaacgtc gccgtcactg tcagcggggc agatcgtagc caaacgttat
    10741 catcatcccg gcggtgccca gcagattgaa ctggacattg gcccgaccgg ccttaaaccc
    10801 caggcccgga atcagtcccg gcgcgacgcc gttgtgattg aagggaatct tgtcctcgtg
    10861 cggcccttta tatcccagga tcaaccctcc ggtcagtttg aaataccagc ccgggtcgcc
    10921 ctcgtgcagc ggccaccacc tgccaccgta gatataatgg ctcttctggt cgaatgagtt
    10981 gttgaaatag gcataaccgg cgagccagcg cgaggggcgt tgccactcgc cccccaccag
    11041 ccacgaatac ttcgcgtgcc cagaatccgg acgaaagtgt atggcgtccg gcgccgcctg
    11101 aaccatgagg acatcgtcgg aaggggtcgg gtcgggcgtc gcggcaaacg ctggaacggt
    11161 tatcgcagca ccggcggcca ccgcaagcag gcaaattcgt cgggcggatt gcatggacgc
    11221 gttatggcaa cgtcggactc aaacgccgtt ttcccgtcag cgcccggccg ccggccgccc
    11281 tcgggctgcc agataccatt tgagaatttc tccgccggtc cagaacaaga cacccttctg
    11341 cttcttgagc cgcctcaaca cggtttcgaa atattttatg cggtgcggca cgccggagat
    11401 gtagggatgc accgcgatcg ccatgatttt cgcgcgctcc ctgccctcgc ggtagacgcg
    11461 gtcgaaataa tccatgcagc gcgtttccca ctcccgggcg gcatggtgct ggaccaccat
    11521 catcgcgatg tcgttgagct ccagcgtgta cggcatcgtc acgagggagc cgttgcgggt
    11581 cttgatcatg cacggctcgt cgtcgatcac ccagtccgcg atgtaccgga tgcccgccgc
    11641 cgccagcagt tccggcgtat cgagctgctc ggtcagcccc ggccccatcc atcccaccgg
    11701 ccgtttgccg gtgaattccc ggatcaccct gaccgatcgc ctgaccattt cacccggatc
    11761 gggctcgccg tgcatcggac gctggtcgta ggaatggccc atgaattccc agccgccgtc
    11821 gcgagcggcc ttggccactt tcgggtaatc caggcacacg cgggcattga tggaaagcga
    11881 cggcacgatg cccaggccgt cgagcgccgc tttcaggcgc cagaatccga cgcgcatgcc
    11941 gtactcgtgc cagccccagt tcgccacgtc gggaagctgg gtgacgccgg tcggcggcgg
    12001 cagcacctgg cgcggcatcg accgcccgac atcccatacc tcgacgttga cgaccggcca
    12061 taccaccacg cgcgcccggt ccggcagtac gagcggcggg cgatcaacga tggcggagta
    12121 gcgcaggcgt ttggcgggga tcatggattt tttctttccg gcaggggaac ttgcggctgc
    12181 cattataact gccgaagcca tcttctcacc ctgacatgct cctcctcatc aagccggaaa
    12241 gaaaaaaatg aatgaacggt tggaaattac aaagcggggc cgaggccgat gatcttgtgg
    12301 tgcgggcgtt gaagtagctg gcgttcggtc atcgcgctcc ccgctttggg gccggccggc
    12361 atcagccgag gccggcccag ctctgtatcc acccggctgg aaagggaaca tggaggagac
    12421 ggtcaaacag cgcgtagaaa aacgcccacg ccgcaagggt gatgagcagg gacaaccccc
    12481 acccttcccg tccctcgacc ttgaggtaaa ggagcacgaa gagcgggacg gcgatcggga
    12541 aaccaaccag cacgatcgca ccgaagaagc cgagcatcca gccggctgcc cgcagcgttc
    12601 tccagaaagc aacccgtgcc ggcagatctc tggacaactg gaaatccatc gcctggtcct
    12661 ggcgcgtggt gccgaacatt acccacagga cttcggccgt cgccaggcag aagaccggga
    12721 tcccgatcac gagtgggaac agtttggcct tccacggcca gtcccaggcg gcaacgactg
    12781 cccatcccga gcagatcatg atgactgaag ctaggaacaa ggacgccctg ctatgcatgc
    12841 gtttccttct tcaagctgga acgccgcgcc tgtagcagcg ggtagagaat caccgcaatc
    12901 gccagggcga agagcaccag cacgatcggc cggtagagcc aggccgtttc gtagcgggcg
    12961 accgagatgt aaaggtagtt ctccgcgatt ttccccagga ccaggccgag gacgaacggc
    13021 gcgcgcggcc agccggccat gaccatgaga tagccgatgc cgccgaagag cagcgtgacg
    13081 agcaagtcgc cgtaatgatt gttggacgtg tagctgccga tgaaaaccag gagcaggatg
    13141 agcgggatga gcagatgtcc gcgcacggcc gtcaggttgg cgaggtgatt gagaaacaga
    13201 aagcacgccc cgacagtgat gatgttggcg agaacaatcg tccaaaccat cgagaacgtg
    13261 acgaccagat gcttcgtcag catgtcgggg ccggggacca accccatcag gaagaaaccg
    13321 ccgagcagaa tcgccatcgc cgagcttcct gggacgccga acgccaccgt cgggatgaga
    13381 gaaccgccct ctttggagtt gttggccgcc cccgggccga ggacgccgcg cacgtcccct
    13441 ctgccgaact gcttgcgctc ttcagcattg cgagcgctct gcgtggcgtg cccgtacgct
    13501 acccactgcg ccacggcccc accaagtccg ggcatgatcc cgatgaaggt gccaatcagg
    13561 ctgcagcgga tcgtgagcca gaagtgccgg aaggtgtcct tgacgccttc aagcgcgccc
    13621 ttgctcaacc ggccggaggg gacgtcgccc gcgatcgagc tgccgcgaac cgccagatcg
    13681 atgatctccg ggatggcgaa aatgccgatc agcaccggca ccaggtcgat gccgccccag
    13741 aggtagagcg agtcaaaggt atagcgcgcg attccggcct gagggtcctg gcccaccgtg
    13801 gcgagcatga gaccgacacc acccgcaaga aagccgcgga gcattcctcg tccgccttgc
    13861 gagctcagcg acgcaatgaa cgcgaggccc acgatggcca gcatgaagag ctcgggcgag
    13921 ccgaagctca ggaccagcgg gcgcaccacc gggaccgata gcgccagagc caatgcgccg
    13981 accagcgcgc cgatcaggga gcttgcgagg actgccccca aagcgcgccc ggcttcgccc
    14041 tttttcgcca tagcgtgccc gtcgagtatc gtggccgccg tggtggcttc accggggacg
    14101 ccgaacaaca ccgaggtgat gtcgccggtc gtcgcaacca cggagtgcat cccgagcaag
    14161 aacgcgaagc cctcgaccgg ctccatcgcg tagacgaacg gcagcatcag cgccagcgtg
    14221 gtcgcgccgc ccagcccggg aaggagaccg acccagaagc cgagcccgat cccgaccagc
    14281 atgaaggcga acgccttcca ttgcaggacg aggagcagac cctcgaagag cgcgtcgatc
    14341 actcgccgaa cttgatgtac ttgctgaacg cgtccacgat gtccttcggc tgggacagaa
    14401 cctcgttcag gaccttgagc gtttcgtcgg cggaaacgta ctcgaactcc atcttggcct
    14461 tcgtggcctc cgcggccagt tccggatcct tgatggcgtt gcggaacgcc tcccgcatca
    14521 tcctgacgac gttatcgggc gtgccgggcg gcatcacgta aggacgcccg atcacctccg
    14581 gggccgagcg cagcgccatg atcgccttgg cgcgcgggtt gggggccagg ttctcgtcca
    14641 ccggaagctg ctcgatcccg gcctcgatgg cacgggcgcg gaccaggggg cggaccaggt
    14701 tgcggtcgat gaaaggtttc agggaagaat acgagcccgc ccgcccgtcc acctccttgc
    14761 gctcgaccgc cagcatgatg tcggagctgc tggaatagcc ggtcacgatc ttgagatcga
    14821 cgccaaggag ttccttcagg agcaggggaa aatcgtaggt gttggccccc ggcccggtcg
    14881 cgccgatcac caccgtcttg ccggctttcc tgagctcgtc gaaactcttg tacggaagat
    14941 cgctgcggat cgtcaggatc gtggtctcac tggcggccga cccgatccag gcgaactttc
    15001 tcatgtcgta cttcaccccg ctcacgctcg tgagctgcgc aagcacgaga ttgcggttga
    15061 atgtcccgat ggtgagcccg tcgggcctgg ccacgctgta gacgtggttg gccgagatga
    15121 tgctgttcgc gcccggcatg ttctgcacga tcacgttggg ctttccggga acgtgcttcg
    15181 ggagatgcct ggcgaggatg cgggccgcgc ggtcgtagcc cccgccggtc ttgaaaccga
    15241 ccacgatcgt gatggttttc ccttcgaagg gggaagtctg ggcctccccg acccgcggcg
    15301 cgagcgccga taacgtgaaa accgcgatga gcgcgagcgt acttctcata aacctctcct
    15361 ccatttcatg tcttaacccg ttatgcgccg gtcctcctcg agcgccggcc ggcccgggac
    15421 gtgttttggc ccgatctccg ggctggggaa cgcatgatat atcacgtttt cccggctacc
    15481 aacccgcaat gagctccggc gacggtttca cttcgccgcg ctcgacgcgc ttcactgctt
    15541 cgttcatctt cgcgtgcatg ggcgcgggca cgccgatctc cgcgccgcgg gcggcgacga
    15601 agccgttgat gaaatcggtc tcggtgcggc ggcccttggc aatatcctgc gccatggacg
    15661 gacgctgatc gtcggagcgc ctcgtagcgt cctccacgat gatatcagtg atctctgcaa
    15721 gcgcgtccct atggccctct ccggcgcgtg cgagagcttc cggttccatg ccttgcatcc
    15781 gctcgagctg gaaacccagc gtctggccca ccctcaccgc ttcgctcccg agacgtatcg
    15841 agacccacct cggcccttcc agcgcgtcgc gctggttgcc gtagagaccg gttgccgcag
    15901 aaagcccgtt cctcatcgag ttgacgacga gcttggacca gcgctcgccc cacaggttgg
    15961 tcgtgacctt tgcgctgtcc accgagcgca gcagctccgc gacctcctcc acgcgccgcg
    16021 tgacgcgccc gtgcggctcg ccgacccgga acacggtgtg ccgctcgccg cccatcggca
    16081 cggtgcgctt gacatggccg gcctgataaa gctccaccgc gatcaggctg gcaatgcagc
    16141 ccaccgtgcg gccccagccg atatgctggg cgatgcgctc ctcgttgatg cagttctgta
    16201 gcgagacgac gaacccgtcg ggcgcgaggt atggccggat caggagcgtt gcccattcgg
    16261 tgtcgtacga cttgacgctc acgaacgcga tgtcgatggg cttttgcttc accaggcctt
    16321 gcacgtcgca cacgtgccgg gcgttgaccg gcaccgaata gcgctccgcc ggcgtcgtgc
    16381 ccgagagctg gatgccattg cgacgcatgt actcgacgtg ttccggccac ggatcgatca
    16441 gcgtcacgtc gtgcccggcg cgcgtgagat gtccgccgac gtagccgccc agggcgcccg
    16501 ctccgatgaa aacgatacgc ttgcccatat gtacccctca aaggcgtgag ccgtgatccg
    16561 tgatccgtga tccgtgatcc gacttcctcc ggttctattc ggctcacggc tcacggttca
    16621 ctgatcacga tttcagtttc tcaatgatgg catcggccat cgcccgcgtg cctgcggttc
    16681 cgccgaggtc gcgcgtcacg gtgcgtcttt cacgcagggc ctgcacgagt gcggtctcga
    16741 tacgatcggc cgtcaccgtt tctccgatgt aacgcagcag catcactgcg gagagtatca
    16801 tcgccatcgg gttggcgatg tccttgccgg caatgtccgg cgccgagccg tgcaccgcct
    16861 cgaacatcgc gcattcggcg ccgatgttcg acccaggcgc gacaccgaga ccgccgacca
    16921 gccccgcggc gagatcggag acgatatcgc cgtagaggtt gggcagcaac aggcagtcga
    16981 aggcttccgg cttcatgacc agttccatgc acagggcatc caccacgcgc tggtccacgg
    17041 tgaatccgga tcccctggcg acatcctgcg cggtcttgag gaacagcccg tcggtcatct
    17101 tcagcacgtt ggccttgtgc acgatggtca cccgccggcg tccgagccgc cgcatggcat
    17161 cgagcgcaaa gcgggtgacg cgctcggtcc cgcgcgccgt gatgatcttg accgcctcgg
    17221 ccgtgccctc gtccaccatg cgctccattc cgacgtagag gtcttcgctg ttctcgcgga
    17281 agatcaggag gtccacatgc tcgaagcgcg aagcgacgcc ggaatagttc ttgaccggcc
    17341 ttacgttgac gaagaggccc agctccttgc gcagcgcaat cgcgacgctg ggataggtcc
    17401 gcttcccgat cttgccgctg gcgtccgcgc gttcgatgtt gacgcggtaa tcgccgccaa
    17461 acggcgtctg ggtcgggccc ttgagcgcga gcctggtcct gtcgatcgaa tcgaacaccg
    17521 agcgcggcaa cggcgtgccg tgctcccgct ccgcgcgctg gcccgcgacg acttcctcga
    17581 actgaaccgg cgcgccggaa gcgagcacga cggcccgcgc cgcggcggtc acctcggggc
    17641 cgatgccgtc accaggtatc aatgtgatcg cgcgcatgca agtaagcggt aattcggact
    17701 tgcgttctgg cggaagttct ccgcttactg ctcacctctc actgcttgcc atgagcaatc
    17761 acgctgtatc gctggaaccc tcccgccagg ccttgagccg cggcagaagg aaaggcaaga
    17821 ggatggacag tatcgaaagc gcgaccaggg tgacgcagat tacgctgccg acgaagattt
    17881 tggggttact gcccgagatc gtcatcgcct ggcggaacga ctgctccatg atgccgccga
    17941 gcaccagtga cagcaccatt ggggcgaccg ggatgtccac cttgcggaag acgtagccca
    18001 tcacgccaaa gacgagcccg atgtagagtt cgaccggatt gccgttgatg gcgtagatgc
    18061 caatggtgga gatcgccatg atcagcgcca tcaggatgcc gctcgggatg tagagcaggc
    18121 gcacgaacag cccgatcagg ggcaggttca gaatcagcag catgatgttg ccgatgtaca
    18181 tgctgtcgat caagccccac accaggtccg ggcggttctg gaacagcaat ggcccgggct
    18241 ggatgccgta catgatgaac gcgccgagca tgaccgcggt cgcgccgccg cccgggatgc
    18301 cgagggtgag caggggcacc atcgcccctg cggtgtcgga attgttcgct gcctcgggtc
    18361 ctgccacgcc ctcgatcgcg ccggtcccga actgctcggg gtgcttggac aggcgctttt
    18421 cccagacata ggaaaggatc gcggcgatcg tgccgccggc gccaggcagg acaccgatga
    18481 tgaagccgat gatgccgccg cgcagcatcg gcatcaccga gcggttccac tcctcgcgcg
    18541 taagccacaa cctgccgctc aaccggatga tctcggggcg tatgcccttg gtcatgtcct
    18601 ccatggtgga aaacacttct ccaatcgcga acagccccac tacggcgatg atgaagccga
    18661 ccccgtcctg cagctccggt actcccatgg tatagcgcgg ctggccactc tgcaggtcga
    18721 tgccgatggt tgcgatcatg aggccgagga tggtcgagat catcgccttc gccggggatt
    18781 taccggcgag cgaggaaacc gccgtcatcg cgaaaaacat cagggtgaaa tattccgcag
    18841 gtccgaactt cagcgccatg ctcgccagcg gcaccgcgaa cagcgtcagt cccacgacgc
    18901 cgagcgtgcc ggcaaagaac gagcccagcg ccgatatcgc gagcgcggcg cccgcgcgtc
    18961 ccttcttcgc catctggtag ccgtcgagcg tggtcatcac cgaggacgct tcgcccggcg
    19021 tgttgatcag gatcgcagtc gtcgaaccac catagcgcgc gccatagaag atgcccgaca
    19081 tcatgatcaa agcggacgtc gggttcatgc cgtaggtgac ggggatcagc agcgcgatgg
    19141 tggccgacgg cccgatgccc ggcagcacgc cgatgatggt accgaggaag cagccgagaa
    19201 aggtatacca gagattgacc ggctgcaggc agaccgagaa gccgttgagg atgtgggcca
    19261 gggtgtccat caaaacggca aaatgccctt cggcaagttg acgtcgagct tgacgaacat
    19321 ccagtagctc agcacgcaga acaacaccga ggtgagcgcg ttcgccggcc atttgccgcg
    19381 gttgaaccaa gccatcagcg cgaacaggta gatcgcggtc gcgacgatgt agcccagctt
    19441 ctcgaacatc gcgtaataga tcccggtcca gaccgtcacg gccgcgatta cacctgcgta
    19501 gcgccattga gcggcttcgg cactggccga ttgcgacgtt cgcgccttgc ggtccttcca
    19561 catctcgacg aacaaaaggc ccgccccgag cagcagaccg atgccgagca ggcgcggaaa
    19621 cgccttggga cccagcgggt caccgatctc gagcgaaggt atcaacgtgg tggcatagag
    19681 atacaccgcg gccacgatga tggtgcagac gaagatgacc cgatcggtca tcatgaaccc
    19741 gctctcctca gccggcgaag cgttgtcgtg attggaaaat gcgccgccgc gtccgcctcg
    19801 tccggcgtgc gacatattac tacatctgtt tgcggtactc cgccggatcg atgcgcgcct
    19861 cgatcagcgt gaagcgccgc gatgcgagcg ctgaacgcgc ggcctttttg agctcggcaa
    19921 cgctttcggc caccaagccg tttccgccgt aggctttcgc aagcgtcgca tagtcgggac
    19981 tgccggtggc gacgccggtt tctggcagct tcatgcgctc ctgcttgagc ttgatcagcg
    20041 acagcgtgtc gtcgcggaag accacgatgg tcacggcaag gcgatgatgg gcgagcaggg
    20101 ccatttcgcc gatcaccatg tcgagaccgg cctccccggt catcgccagc accggccgtt
    20161 tcggatggag cagcttggct gcgatcgccg ccggcaacgc gtatcccatc gagccgagcc
    20221 cgttcgactg cagcagccgc cgcggctcgt acgaccgcca gacgtggttg atcaggatgc
    20281 ggtgcgagcc cgtgtccact gttgcaatgg tttcgcgcgg gaacacctcg cgcagcgttg
    20341 tcgtggcctg atacgggccg agaccgcgcc gcggttggtg cgcgaccgac tcccgtatcg
    20401 cctcacggta gcgctgaagc tcgccatccg gccaccgccg cggcggcttc gccggcgctg
    20461 ccgctgcgag ctgcccgagc gcggcggtga tggcgcctgc atactcgagc ccgcagcggt
    20521 agacatgatg cgtgttcgga acgaggtcga acgacatcgt gcgcttgctc ctgtcccacg
    20581 gagcgatcca gtcgctcctc agttccaccg gatcgaagcc gatgcacagg acgaggtcgg
    20641 cgtcccgaat ccgcgccatg tggatcgcgt ccaccaccgg ggagagaccg gcgccgccga
    20701 tgcagagcgg gtggtcctcg gggatcacgc cttttgcctt gtaggtggtg acgacgggta
    20761 cgcgccatcg ctcgagaaaa tatctcagtt ctttcgcccc gctatcgagc tgcacgccga
    20821 ccccgaccag cgcgagcggc ctgcgagccg tcctgagcca gccgatcgcg cgcgacaaat
    20881 cctgtgccgg cggctggcct gcaatgcttg ccgtcgcaaa gcgcccgccg gtctcctgct
    20941 ccatggccgc aacatcggtg ggaacgttga agtgcaccgg ccccggcatg ggcgtgagcg
    21001 cgatggcaat gcctttgcga gcctgctcga gcgcgccgcg cgcggcgaag gtcgcggacc
    21061 acttggtgag cggcccgagg atcccgcact ggtcgatgat ctggtgcgtg tagatgctcc
    21121 tcaacgccgc ggcgatctcg cctgtgatga cgatcatggc ggagcgatcg agcagtgcct
    21181 gagcgaccgc cgtcacggta ttggtcaggc ctgggcccag cgtcgccacc agcacgccgg
    21241 gcttgccggt gagctgaaag gccgcatcgg ccatgaaacc ggcgcccagc tcctgcttgg
    21301 tgagaatgaa gcggatgccg gccttgcgga aagcctcgag cagctcgagc acttcgccgc
    21361 cgggaatgcc gaaagcatgg cggacgccgt gctctttcag cgcgcgggcg aggatgtcgg
    21421 cggtcttggg caatgcggtc ttcccgtggt gttacgaacg gaagcgctgg atagcatgtg
    21481 cggcgccggt ggatggtagc acagccgcac gcggcgtctc gtgcgccggc cacaggcatc
    21541 agttcacatc gtgtcggccg catgaacgcc cgcgcggttt cattctcccc ggggacaagt
    21601 ttcctgggcg ccgaacccga tctcgattga ccctttgtcg aatcggtctt ttccatatcg
    21661 tggagaacgc tatcccgccg gcgtggcgca atatataatt tcctttcccc gacggcaggc
    21721 gggtgtaacg ccacgaatcg aaacgccaag cgccgtcgct ggcgaaaaga atatccagga
    21781 gcccacatga gccacaacct gttcaactcc cttcaggaat tcaagtacgc cagcaagacc
    21841 ggaaagtatt actcgctgcc ggcgctggaa aaggccgggc tcggcaagat ttcccgcctg
    21901 ccgatttcgc ttcgcgtcgt gctcgagtcc gcgctgcgca actgcgacgg caagaagatc
    21961 accgaggcgc acctgaagaa catggccggc tggaagccca aggctaagcg caccgaggag
    22021 gtccccctgg tcgtcgcgcg cgtgctgctg caggacatga cgggggtgcc gctggtcgtg
    22081 gacttcgccg ccatgcgcga agccgcgaaa gtcatgggca agaaccccga gatcatcgag
    22141 ccgctctgcc ccgggcacct gatcatggac cactcggtgc agatcgacaa ttacggcacc
    22201 cccgacgcgc tcaggaagaa catggagatc gaattcaagc gcaaccgcga gcgctaccag
    22261 ttcctgaaat ggagcgcgag cgcgttcaag accttccgga tcatcccgcc cggcaacggc
    22321 atctgccacc agatcaatct cgagtacgtt tcgcaggtgg tgtggcagaa ggacggcatg
    22381 ttttaccccg acagcttggt cggcatcgat tcccacacca cgatggtcaa cggcctgggt
    22441 gtgctgggct ggggcgtggg cggcatcgag gccgaggccg cgatgctggg ccagccgatg
    22501 tacttcctcg agcccgacgt cgtcggcgtg cacctgaccg gcaaactgaa tgacggcgtc
    22561 accgccaccg acctcgtgct cacgatcgtc aagctgctgc gcgacaccaa ggtcgtcggc
    22621 aagttcgtcg agtatttcgg cgagggggcg gcctcgctcc cggtgccgga tcgcgccacg
    22681 atcggcaaca tgtctccgga atacggcgcg acgtgcggct tcttcccagt ggacgacgtc
    22741 tcctgcgact tcctcaagat gaccgggcgc ggcaacatcg tgccggcggt caaggcgtac
    22801 tggaaggcgc agggcatgta cggcatgccg aagaagggcg acatcgacta cagccagatc
    22861 gtcgagctcg atctgtccac ggtcaagccc gcggtgtcgg gcccgcgccg cccgcacgac
    22921 catctcgacc tgccgaagat cggcgcgaag taccgcgagc tgatggggaa gcccttcacc
    22981 gacggcggct acggcaagcc caacgaactc ggcaagcggg tggcaacctc ccggtccggc
    23041 gtggacgtgg gccacggcga cgtgctgatc tcggccatca cctcctgcac caatacctcc
    23101 aaccccggcg tgctcctcgg cgcgggcctg ctcgccaaga aggcggtcga aaagggcctg
    23161 aagccccacc cgcgcgtcaa gacctcgctc gggccgggct cgctcgccgt gaccgagtac
    23221 ctgaaaaacg cgggtctgct gccgtacctc gaacaacttg gctattacgt cgcgggctac
    23281 ggctgcacca cctgcatcgg caactcgggt ccgctcgacg cccacatcga cgaggcggtc
    23341 gcgaagaacg acatcgtcgc cgcggcggtc ctgtcgggaa accgcaattt cgaagcacgg
    23401 atccaccaga acatccgagc gaatttcctg atgagcccgc cgctggtggt cgcattcggg
    23461 ctggccggca ccatcgacat cgacatgtcg aaggacccga tcggcaccgg caaggacggc
    23521 aagccggtct acctcaagga catctggccc tcgacccggg aggtcggcgc ggcgatgaag
    23581 tacgcgaccg atcccgaaat gtaccgcaag ctgttctccg ctttcggcga aggcaacccg
    23641 atgtgggacg agatccccgc gtcggtcggc gccacctacc agtgggacga gaaatccacc
    23701 tacgtgcggt tgccgcccta cttcgagggc ttctcgatga agcccggcaa ggtcaccgac
    23761 atcaggggcg cgcgggtgct gggcatcttc ggcgacgcga tcacgaccga ccacatcagc
    23821 ccagccggcg gcatccgccc caccgcaccg gctggcaagt acctgcagga ccacggggtg
    23881 aagatccccg acttcaacac ttacggagcg cgccgcggca accacgaggt catgatgcgc
    23941 ggcaccttcg cgaacatccg catcaagaat ctcatggtgc ccggcactga aggcggcatc
    24001 accatccacc acccgtccgg cgagcagacc tcgatctacg aggccgccat gcgctacaag
    24061 aaggaaggcg tgccgtcggt ggtgttcggc ggcgatgagt acggccaggg ctcctcgcgc
    24121 gactgggcgg ccaagggacc gcagttgctc ggcgtgaagg cgtgcatcgt gcgcaacttc
    24181 gagcgcatcc accgcgcgaa cctcgtgttc atgggcgtgc tgccgctgga attcaaggac
    24241 ggcgccactg ctcaatcgct gaagctcgac ggaagcgaga ccatcgatct cctcggcatc
    24301 gagaaaggca tcaccccgat gcaggacgtc accatggtga tccaccgcaa gaacggtgac
    24361 gcgacaaagg tgccgttgaa gctgcgcatc gacacgccga tcgaggtgga ctactaccag
    24421 cacggcggca tcctgccgta cgtcctgcgc cagctgatcg cggccgcgta acgagcaagg
    24481 ctcgagccgg caccatacgc ggtaccggct gtctcgcagg ccggcatcca caagatgccg
    24541 gccggttttt tccagacttt ataatcatcg catggccagt tctgcgcgct cggcggtccc
    24601 tgcctccctc ctggagaggg tcggttccat cgtcggtcca cagggcctga tcaccgacct
    24661 tcgagacatt gagccgcaca tcgtggactg gcgtggcctt taccgcggcg ccacacccgc
    24721 cgtggtgcgt cccgccagtt ccgccgaagt cgccgaggta gtaaaggcct gcgccgagac
    24781 gcgcacggcc gtcgtgccgc aaggcggcaa caccggcatg tgcggcgcct cggtgccgcg
    24841 cgcggaaggc ggggaaatcg tgctcaatct cgcgcgcatg aaccgcatac tcgaagtaga
    24901 tgcgctgaac aacgccgtca gcgtgcaagc cggctgcatc ctcgccaaca tccagcaggc
    24961 ggcgcgcgac gccggacgat tgttcccgct ttcgctcggc gcggaaggca gctgccagat
    25021 cggcggcaat ctctcgacca acgcgggcgg agtgaacgtc ctgcgctacg gcaacgcgcg
    25081 cgacctcgcg ctgggactcg aggtcgtcct gcccgacggc cgcctgtggg atggcatgcg
    25141 cagcctgcgc aaggacaaca ccggctacga tctcaagcag gtgttcatcg gcgccgaagg
    25201 cacgctgggc atcatcaccg ccgccgtgct gaagctcttc cccctgcccc attccggcgc
    25261 gacggcctgg gccgcggttt cctcgccgga agccgcgctg aagctgcttg cgctgctgcg
    25321 gcagcagtgc ggcgacaagg tgacggctta cgagctgatc tcgcgcaact gcctcgatct
    25381 cgttctgcgc cacatccccg gcacgcgcga tcccctggcc gggcggcacg cgtggtacgt
    25441 gctcgccgat ttgagcgatg aacgcgaaag cggcgcgatc cgcggagagc tcgagcaagt
    25501 gctcgcggcg gcaatcgagc gtgggttggc gagcgacgcg gtgatcgccg agagccagtc
    25561 ccaggcgcag gggctgtggc ggctgcgcga ggcgataccc gaggccgcgc gcgccgagcc
    25621 cggaatgctc taccgccatg acatcgcggt cgcggtgagc agggtcccgg cgttcatcgc
    25681 ggaggcacag gccgccctgc aattgcggtt ccccggcgtg gacgtgatct gcttcggcca
    25741 cctgggcgac ggcaacctgc attacaacgc cttcgtcccc gggcgccgcc gcgaggacgc
    25801 ctcggcgcgg gaggcccgcg atgtcacgca aatggtctac gacatcgtga gccgctacca
    25861 cggcagcttc agcgccgagc atggcatcgg ccagtacaag ctcgcggaga tcatccgcta
    25921 caagggcggc gtggaactcg agatgatgca cgcgctcaaa agggctttcg acccccataa
    25981 catcatgaat cccggtaagg tccttcctga gtgatgggtg atgcgtgatg ggtgatgaga
    26041 tgacggccaa aatcgagaag agcgacgcag agtggcgcaa gcagctcacg cccgagcagt
    26101 ttcatgtcgc tcgtgaaaaa ggcaccgagc gtgcgttctc cggcgcctac tggaaccacc
    26161 acgagcccgg cgtctaccgc tgcgtgtgct gcggggtcga gctgttcagc tccgagcaca
    26221 agttcgattc cggcaccggg tggccgagct tctggcaacc ggtgggccgt gagaacgtcg
    26281 cgaccgagga cgaccgcagc tttttcatgc atcgcaccga ggtgttgtgc ggccgctgcg
    26341 acgcccacct cgggcacgtc ttcgacgacg gaccgcagcc cactggactg cggtattgca
    26401 tcaactccgc ttcgctcaag ttcgagagac ggtaagcagt gagcggtaag cggaaaacac
    26461 cgattcttga gttccgttcc gccaaccgcc agctgctcac cgctgaccgc ttacggttat
    26521 aatacgcgcc catgaaattc ctgttcgaca tcttcccggt cgt
//
LOCUS       DBNGWZ010000464         1717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2076508, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000464 DBNGWZ010000000
VERSION     DBNGWZ010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2076508"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /gene="accB"
                     /locus_tag="AC16CX_14245"
     CDS             <1..524
                     /gene="accB"
                     /locus_tag="AC16CX_14245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR3013703.1"
                     /translation="HQGRYSMEWTEQQILETLRLIEDSDFDEVKLETENFKLHVRKTG
                     TPAAWAEIQAPPGASDARASPENATAKKPAATAQPGAGRAGNEEAAIPEGMVAIRAPM
                     IGTFYRAPAPHLAPFVEVGASVKPEDTVCLVEVMKLFNTIKAGVAGKVVKILAQNAAT
                     VERDQVLMLIEPS"
     gene            535..>1717
                     /locus_tag="AC16CX_14250"
     CDS             535..>1717
                     /locus_tag="AC16CX_14250"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3013704.1"
                     /translation="MTIKRVLIANRGEIAVRIARACRKLGVEAVLACSEADRASLASR
                     MVDRIVCIGPAQAMQSYLDQDAVITAAIGAGCDALHPGYGFLSERASFRRLCDEGGVR
                     FIGPHAEAIATMGDKLTALRLAKANGVPVIPGCDELKSARDAKTEARRAGLPVILKAS
                     AGGGGRGMRVVRALKEIEGAFASASAEARAAFDDGTLYMENYIERARHIEVQVFGDTH
                     GNIVHLGERDCSVQRRHQKLVEEAPSPVVDGPLRKSLTASALRLAKAASYAGAGTVEF
                     IVDLDSKAYYFLEMNTRIQVEHPVTEEITGIDLVSEQIRVAAGEPLSFSQGDVKLSGH
                     AIECRINAEEVGAGFMPCPGAIVEWQQPDAAGVRVDTHCYPGYAIPPFYDSMIGKLIV
                     RG"
BASE COUNT          357 a          506 c          567 g          287 t
ORIGIN      
        1 atcatcaggg ccgatacagc atggaatgga ccgaacaaca aatactcgaa acgctccggc
       61 tgatcgaaga ctccgatttc gacgaagtca agctcgaaac ggaaaatttc aagctgcatg
      121 tgcgtaagac ggggacgcct gcagcctggg cggagattca agcaccaccc ggcgcgtcgg
      181 acgcacgcgc gtcgcctgag aacgccaccg cgaagaaacc cgctgcaacc gctcagccag
      241 gcgcgggccg tgcaggcaat gaggaagccg ccatcccgga agggatggtg gcgatccggg
      301 ctccgatgat cggaaccttt taccgcgccc cggcgccgca tcttgcaccg ttcgtcgaag
      361 ttggagccag cgtcaagccg gaagataccg tttgcctggt ggaggtcatg aagcttttca
      421 atacgatcaa ggccggggtc gcaggcaaag tcgtgaagat actcgcgcag aatgccgcca
      481 cggttgagag ggatcaggtc ctgatgctga tcgaaccgag ctgagggcgc cacggtgacc
      541 ataaaaaggg tgctgatcgc gaaccgcggc gagattgcgg tgcgtatcgc gcgcgcttgc
      601 cgcaagctgg gagtcgaggc cgttctggcc tgttccgagg cggaccgggc ttcactcgcg
      661 agcaggatgg tcgatcgcat cgtatgcatc ggccccgcgc aggcaatgca aagctacctc
      721 gatcaggatg cggtcatcac tgcggcgatt ggagccggct gcgatgccct gcatcccggg
      781 tatggctttc tttccgagcg cgcctcgttc cggcgtctgt gcgacgaggg gggagtaaga
      841 ttcattgggc ctcacgccga agcgattgcc accatgggcg acaagctcac ggctctgagg
      901 ttggccaagg cgaatggtgt gccggtcatt ccaggctgcg atgagctgaa gagcgccaga
      961 gatgcgaaga ccgaagccag acgagcaggg ttgccggtta tcctgaaggc gagcgcgggc
     1021 ggcggcggca gaggcatgcg tgtggtgcgc gccctgaagg aaatcgaggg cgccttcgcg
     1081 agcgcatcgg ccgaagcacg ggcagcgttc gacgacggca ctctttacat ggaaaactac
     1141 atcgaaaggg ctcgccatat cgaagtgcag gtcttcggcg atacccatgg caacatcgtt
     1201 catctcggag agcgcgattg ctccgtgcag cgcagacatc agaaactcgt cgaggaggcg
     1261 ccgtcacccg tggtcgatgg tccgctgcgc aagtcgttga ccgcttccgc cctccggctt
     1321 gcgaaggccg cgtcctatgc cggagcagga accgtcgaat tcatcgtgga cctggacagc
     1381 aaggcctact atttcctgga gatgaacacc cggatccagg tcgagcaccc ggtcaccgaa
     1441 gaaatcaccg gcatagacct ggtgagcgag cagatccgcg tcgcggcagg cgagccgttg
     1501 tccttctcgc agggcgacgt caagctgtcc gggcatgcca tcgagtgcag gatcaacgcc
     1561 gaggaagtgg gcgccgggtt catgccctgt ccgggggcga tcgtcgagtg gcaacagccc
     1621 gacgcagccg gggtacgcgt ggacacccac tgctatccgg gatacgccat cccgccgttc
     1681 tacgactcga tgatcggcaa actgatcgtg cgcggcg
//
LOCUS       DBNGWZ010000465        12116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_942728, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000465 DBNGWZ010000000
VERSION     DBNGWZ010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_942728"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2049)
                     /locus_tag="AC16CX_14255"
     CDS             complement(1..2049)
                     /locus_tag="AC16CX_14255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR3013705.1"
                     /translation="MNPLLDFSGLPRFADFKPELVTPAVEQLLAENHALIGRISVPDV
                     PSTWDGFVQPLEDANERLHRAWGVVGHLNGVMNSPELREVYNANLPRVTQYYTELGQH
                     EGLFAKFKALRADPGFEGLARAQKKIVENELRDFRLGGVELPPEKKARFKEISERLSQ
                     LSSRFSDNLLDATNAFAHYLTDAQEAAGIPQDVLAAAREAAQADGKPGWKFTLHAPSY
                     LPVMQYADNRALRERMYRAYVTRASEFGKPEWDNTSLITEILRLRAEQAPLLGFANYA
                     EYSLEPKMADSPRQVLDFLDELASRAKPYAERDLKEVTEFARAELGLTRLETWDLAYA
                     SEKLRLKRYAFSDQEVKQYFPETRVLPGMFKLVETLYGLRIEPAQAPVWHPDVRFYAI
                     RDSAGSLVGQFYVDLYARPSKRGGAWMDDAITRRARDGRVQTPVAYLNCNFSAPVGGK
                     PALFTHEEVNTLFHEFGHGLHHLLTRVDYLGVSGINGVEWDAVELPSQFMENFCWEWD
                     VLAPMTSHVDTGASLPRALFDKMRAAKNFQSGMQIVRQIEFALFDLRLHHDFDPHGAR
                     TALDLLNEVRSRVAVVFPPDYNRFPHNFSHIFAGGYAAGYYSYKWAEVLSADAYSLFE
                     ENGVLDARIGQRFWNEILGMGGSRPAHESFVAFRGREPKIDALLRHSGMSGEAGAQTL
                     "
     gene            complement(2151..3425)
                     /locus_tag="AC16CX_14260"
     CDS             complement(2151..3425)
                     /locus_tag="AC16CX_14260"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3013706.1"
                     /translation="MKIHIKGGRLIDPKSGVDARKDVYVAAGKIVGIGEPPNGYAANR
                     VIDAAGLIVCPGLVDLAARLREPGFEYMATLESEMEAAIAGGVTSLACPPDTDPPLDE
                     PGLVEMLKFRAKNLNQARVYPVGALTQGLKGERLTEMAELVDAGCVAFSQADAAFADS
                     QVFYHALQYAATFGFPVWLRPQDPALARHGVAHDGQVATRYGLPGIPVCAETVALSAI
                     LILARETGARVHLARLSSAEGVEMVRQAKRQGLPVTCDVAIHHAHLTEMDIGYFDSNA
                     RVDPPLRSQRDRDSLRAGLADGTVDALCSDHTPVDEDAKHLPFAEAEAGATGLELLLP
                     LTLKWAEQSKVPLASALAKITSEAARVLNVDAGLLAAGHAADICVFDPERHWKVEPSA
                     LKSQGKNTPFTGLELKGRVRYTLVDGQVVYEL"
     gene            complement(3514..4467)
                     /locus_tag="AC16CX_14265"
     CDS             complement(3514..4467)
                     /locus_tag="AC16CX_14265"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3013707.1"
                     /translation="MWLHPSNPQLNKKGQLHHLLTLEGLPTEILWQILDTAKTFVGVK
                     VKKLPLLRGKSVFNVFFEPSTRTRTTFEIAAKRLSADVINLNVAVSSQSKGESLLDTV
                     DNLSAMHADMFVVRHGESGAPHFIARHVAPDIHVINAGDGRHSHPTQGLLDMYTIRHY
                     KGDFSNLRVAIVGDVLHSRVARSQIHALTTLGCPEIRVIAPKTLLPANVEQLGVHVHH
                     DMRRGLKDVDVVMMLRLQNERMQGALLPSAQEFYKYYGLTDDKLALARPDAIVLHPGP
                     MNRGVEIDSSVADGKQSVILPQVTFGIAIRMAVMSIMAGNK"
     gene            complement(4469..4978)
                     /gene="pyrR"
                     /locus_tag="AC16CX_14270"
     CDS             complement(4469..4978)
                     /gene="pyrR"
                     /locus_tag="AC16CX_14270"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3013708.1"
                     /translation="MPGNPLPDAEQLLKALIEQMRPAVGPETGLIGIHTGGVWVAERL
                     HQALKLQTPLATLDVSFYRDDYEARGLHRDVKRSEIPFEVEGRDLILVDDVLNTGRTI
                     RAALNEIFDYGRPAAVRLATLVDRGGRQLPIAAQFAGATLALKPGQSIGLKRDAKGRL
                     TLELREEAK"
     gene            complement(5136..5591)
                     /gene="ruvX"
                     /locus_tag="AC16CX_14275"
     CDS             complement(5136..5591)
                     /gene="ruvX"
                     /locus_tag="AC16CX_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3013709.1"
                     /translation="MKTPHASHLTPRPRTGTVLAFDFGTQRIGVAVGDFETRLAHPLT
                     TVSAAENRARFAAIERLIVEWRPALLVVGLPAHPDGAEHPVGRLARRFAQRLQGRFGI
                     RTELVDERLTSHEAEGMLRAAGARGARLKAGLDAVAAQRILETYFATAK"
     gene            complement(5662..6222)
                     /locus_tag="AC16CX_14280"
     CDS             complement(5662..6222)
                     /locus_tag="AC16CX_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3013710.1"
                     /translation="MQKMNLTQHFLIAMPNMADPHFTKTLTYICEHNDQGALGIVVNR
                     PIEMTLHALLEQVSIPLASEAFKSIPIHFGGPVQVDRGFVLHTPLGQWQSTLAVSSEI
                     GLTTSKDILQAVARGEGPGKLLVTLGYAGWAPGQLEHELTQNAWLTVQARPEVIFDLR
                     AEERLPAAMGLLGIDFASLSEQAGHA"
     gene            complement(6544..8286)
                     /locus_tag="AC16CX_14285"
     CDS             complement(6544..8286)
                     /locus_tag="AC16CX_14285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3013711.1"
                     /translation="MRKRSRTAEPEAPLAGLQAWSEQVRALNDTTDPFDIAASFNKVA
                     REWLGHPRELSETLSTLAVELHRLQLHAWQVAAGLEPQRPAVATHNDERFADPAWSEV
                     PTFSLLKQYYLLYTRWLEDALYDTPGASARERRRAAFWARQWLDAVAPGNYLLTNPVA
                     LRKFWETGGESLVRGLKNWLDDVRVGDIQMVDRSAFKVGGNLATTPGAVVYRNSLMEL
                     IHYAPATGEVHALPVVFVPPWINKYYILDLTEKKSLVRHLVNQGFDVYMVSWKNPTAD
                     MAETTFDAYLMDGIRRAVDVARAVSGQPRVHAAGYCIGGTALAAMMAWYSREHANPDD
                     VPVAHWTLLTTLLDFSRPGAVEVFIDESTISVLEDMMARQGYLDGREMGRTFRMLRPN
                     TLIWHYFVHGYLYGETPPAFDVLYWNTDVTRMARAMHSFYLREFYLHNRLIRKDGVTL
                     AGHPIDLALIRQPLYAVGCEEDHIAPWKATFKNTSTISAPSRYTLTSSGHILGIINPP
                     VKPPKRSYWTGAATDAPPEDWLKEQKEIHGTWWEDWVQWLAARCGGRVKLPPTATDRY
                     PKLADAPGTYVQEG"
     gene            complement(8297..9718)
                     /locus_tag="AC16CX_14290"
     CDS             complement(8297..9718)
                     /locus_tag="AC16CX_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3013712.1"
                     /translation="MVIMIFSSTMLVPLAVAFFYHDAALYAYDQSVLITLVCGAVLWI
                     ATRQARRELQVRDGFLLVTLVWALLPAFSALPLMFFLPGLSFTDAYFECMSGMTTTGA
                     TVLSDLDQLPPSINIWRGFLQWLGGMGVIVLAVAILPLLGVGGRQVFRAETPGPMKDT
                     KLTPRITETAKGLWFVYSLITLACIFSYWAAGMTPLDAVMHSFTTMSLGGFSTHDASF
                     GFFDSPLIEAVSTIFMLVAGMNFATHFLALRKRSFEPYRLDPEVRWLLIIVLGSCALI
                     AVFLWQKGVYPDFLTALRRATFNTVSLATTTGYASVDFNQWPVFAPMVMLMLSCIASC
                     AGSTGGGIKMMRFQLLFLQGLREMVKLLHPQAQVPVKLGGQVVANQIVFAVLAFMTLW
                     GASVTVMTLLLLVSGLDFVSAFTAVIACITNTGPGLDKVGPATTFAVLSDFQTWVCAI
                     AMLIGRLELFTVLVIFTPAFWRK"
     gene            complement(9825..11240)
                     /gene="trkA"
                     /locus_tag="AC16CX_14295"
     CDS             complement(9825..11240)
                     /gene="trkA"
                     /locus_tag="AC16CX_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HIR3013713.1"
                     /translation="MKIVILGAGQVGSTVAESLASEANDITVVDIEPGRLKRLQDRLD
                     LRTVTGNAAHPAVLEEAGARDADMLLAVTQSDETNMVACKLAATMFNIPTKIARIRSA
                     DYLSHPEIFSPENFAVDHSICPEQILCDYIVKLLEFPESLQVLEFADGKVSLAAVRAF
                     HGGPLVGHEIQHLRQHMPQVDTRVAAIFRQDSPIAPEGRTVVEAGDEVFFIAATGNIR
                     NVLRELRRMDRPVKRVMVGGGGNIGRRLAQAIEDKYEVKIIEFNKTGAERLAAELDKT
                     LVLSGDVTDEELLDAENVSEMDVYCAVTNDDETNIMSSLLAKRRGARKVISLINRSSY
                     VNLVQSGQIDIAISPAQATIGTLLAHVRRGDCAVVHSLRRGAAEALELIAHGDRKSSK
                     VVGRRIEELDLPKGVTIGAIVRRRDRSGTPAERNGDDSDVIIAHHDTIIESDDHVIVF
                     VVNKRIVPKIEKLFQVDVGFF"
     gene            11371..12105
                     /locus_tag="AC16CX_14300"
     CDS             11371..12105
                     /locus_tag="AC16CX_14300"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR3013714.1"
                     /translation="MTRIYFSGAITAGARCELPPVQARHVARVLRLRTGDAVTLFNGD
                     GAEYAAVVERVAKGGVTAKVTGRRLVERESRLEIVLGQALSSGERMDYTVQKAVELGV
                     SAVHPLAAARSVVRLSAERAGKRVSHWQAVAIAACEQCGRNRVPPVAPVVALDEWLSR
                     HNANHDAMLSVLLSPEAETRLRDLPKPDGAIVLLAGPEGGFNPEEESLARASGFTPVR
                     LGPRVLRTETAAIAALAAMQALWGDF"
BASE COUNT         1995 a         3886 c         4148 g         2087 t
ORIGIN      
        1 ctaaagcgtt tgggcacctg cttcgccgct catgccgcta tgccttaaca acgcgtcgat
       61 cttcggttcg cgcccgcgga aggcgacgaa tgactcgtgc gcggggcggc tgccgcccat
      121 cccgaggatc tcgttccaga agcgctgccc gatccgggca tcgagcacgc cgttctcctc
      181 gaacaggctg taggcgtccg ccgaaagcac ctcggcccac ttgtagctgt agtagcccgc
      241 cgcgtagccg ccggcgaaga tgtgcgagaa gttgtgggga aagcggttgt agtccggcgg
      301 gaacacgacg gcgacgcggc tgcgcacctc gttcagcagg tcgagcgcgg ttctcgcgcc
      361 gtgcgggtca aagtcgtggt gcaggcgcag gtcgaacagc gcgaactcga tctggcgtac
      421 gatctgcatg ccgctctgga agttcttggc ggcgcgcatc ttgtcgaaca gcgcgcgcgg
      481 cagcgatgcg ccggtgtcca cgtggctcgt catcggggcc agcacgtccc actcccagca
      541 gaagttctcc atgaactggg aggggagctc caccgcgtcc cactcgaccc cgttgatgcc
      601 ggagacgccg aggtaatcca cgcgcgtgag gaggtggtgc aggccgtggc cgaattcgtg
      661 gaacagcgtg ttgacctcct cgtgcgtgaa cagcgcgggc ttgccgccca ccggcgccga
      721 gaagttgcag ttcaggtaag cgaccggggt ctgcacgcgc ccgtcccggg ccctgcgcgt
      781 gatcgcatca tccatccacg cgcccccgcg cttggagggg cgggcgtaaa ggtccacgta
      841 aaactgcccg accaggctgc ccgcgctgtc gcgtatcgcg tagaagcgca cgtccgggtg
      901 ccagaccggc gcctgcgccg gctcgatcct gagcccatag agcgtttcca ccagcttgaa
      961 catgcccggc agcacccgcg tctccgggaa gtactgcttc acttcctggt cggaaaaggc
     1021 gtagcgcttg agccgcagct tctccgaggc gtaagcaagg tcccaggttt cgagccgggt
     1081 gagcccgagc tcggcgcggg cgaactcggt cacttccttg aggtcgcgct cagcgtaggg
     1141 ctttgcccgg gacgcgagtt catcgagaaa atcgagcacc tgccgcggcg aatccgccat
     1201 cttgggctcg agggaatatt ccgcgtaatt ggcgaaaccg agcagcggcg cctgctccgc
     1261 gcgcagcctg aggatctccg tgatgaggga ggtgttgtcc cattccggct tgccgaactc
     1321 ggaggcgcgc gtcacgtagg cacggtacat ccgctcgcgc agcgcgcggt tatccgcgta
     1381 ctgcatcacc ggcaggtagg agggcgcgtg cagggtgaat ttccaccccg gttttccgtc
     1441 ggcttgcgcg gcttcgcgcg cggcggcgag cacgtcctgc gggatgccgg cggcctcctg
     1501 cgcgtcggtg aggtagtgcg cgaacgcgtt ggtggcgtcg agcaggttgt cggaaaagcg
     1561 cgaggaaagc tgcgacagcc gctcgctgat ctccttgaag cgcgctttct tctccggcgg
     1621 cagttccacg ccgccgagcc ggaaatcgcg cagctcgttc tcgacgatct ttttctgcgc
     1681 gcgcgcgagg ccctcgaaac cgggatccgc ccgcagcgcc ttgaacttgg cgaacagccc
     1741 ctcgtgctgg ccgagctcgg tgtagtactg ggtgaccctg ggcaggttgg cgttgtagac
     1801 ctcgcgcagc tccgggctgt tcatcacccc gttcaggtgg ccgactacgc cccatgcgcg
     1861 gtgcaggcgc tcgttggcgt cctccagcgg ctgcacgaag ccgtcccagg tggacggaac
     1921 gtcgggcacc gagatgcgcc cgatcaacgc gtggttctcg gcgagcagct gctccaccgc
     1981 cggcgtgacc agctcgggct tgaaatcggc gaagcggggc aggcccgaga aatcgagcag
     2041 gggattcatg gaatcagtgt tgagtgttga gtgttgagtg ttgagtgttt agttggtgat
     2101 gcggctgccg ggttcaagcc gccgaaaact caacactcaa cattcaacac tcataactca
     2161 tagacgacct ggccgtcgac cagggtgtag cgcacccgtc ctttgagctc gaggccggtg
     2221 aagggggtgt tcttgccctg gctcttcagc gcgctgggct cgactttcca gtggcgctcg
     2281 gggtcgaaga cgcagatgtc ggcggcgtgg cccgctgcca ggagaccggc gtcgacgttc
     2341 agcacgcgcg ccgcctccga agtgatcttg gcgagcgcgg atgcgagcgg caccttggac
     2401 tgctccgccc atttgagcgt gagcggcagc aggagttcga gcccggtcgc accggcctcg
     2461 gcttccgcga acggcaggtg cttcgcgtcc tcgtccaccg gcgtgtgatc cgagcacagc
     2521 gcgtccaccg tgccgtcagc gagccccgcg cgcagtgaat cgcggtcgcg ctggcttctc
     2581 aacggcgggt caacgcgggc gttggagtcg aagtagccga tgtccatctc ggtcaggtgc
     2641 gcgtggtgga tggcgacgtc gcaggtaacg gggagtccct gccgcttcgc ctggcgcacc
     2701 atctccacgc cttcggcgct ggagagccgc gcgagatgca cgcgcgcgcc ggtctcgcgc
     2761 gcgaggatca ggatggccga gagcgccacg gtttccgcgc acaccgggat gcccggcagc
     2821 ccgtagcgcg tggcgacctg cccgtcgtgg gcgacgccgt gccgcgcgag cgcggggtcc
     2881 tgcgggcgca gccataccgg gaagccgaag gtcgcggcgt actgcagggc gtggtagaac
     2941 acctggctgt cggcgaaggc ggcgtcggcc tgcgagaacg cgacgcagcc cgcgtccacc
     3001 agctccgcca tttcggtcag gcgctcgccc ttcaggccct gggtgagcgc gcccaccggg
     3061 tagacccgcg cctggttcag gttcttcgcc cggaacttga gcatctcgac cagtcccggc
     3121 tcgtcgagcg gcgggtcggt gtccggcggg caggcgaggc tcgtcacgcc gccagcgatc
     3181 gccgcctcca tctcagattc cagcgtcgcc atgtattcga agccgggctc gcgcagccgc
     3241 gcggcgaggt ccaccagccc ggggcagacg atgaggccgg ccgcgtcgat cacgcggttg
     3301 gcggcgtagc cgttcggggg ctcgccgatt ccgacgattt tgccggcggc gacgtagacg
     3361 tccttcctcg cgtcgacgcc gctcttcgga tcgatcagcc ggccgccctt gatgtggatt
     3421 ttcatgatcc agctgtcagc gtacagctat cagcgatcag caaacagcta gaagcaggtt
     3481 caagctgaac gctgaatgct gattgctgat tgctcatttg ttccccgcca tgatgctcat
     3541 caccgccatg cggatcgcga tgccgaaggt gacctgcggc aggatcaccg actgcttgcc
     3601 gtcggcgacg ctggaatcga tctccacgcc gcggttcatc gggcccgggt gcagcacgat
     3661 ggcgtcgggc ctggcgagcg cgagcttgtc gtcggtgagc ccgtagtact tgtagaactc
     3721 ctgggcggaa ggcagcagcg ccccctgcat gcgctcgttc tgcagccgca gcatcatcac
     3781 gacgtccacg tccttcaggc cccgccgcat gtcgtgatgg acgtgcacgc cgagctgctc
     3841 gacgttggcg ggcagcagcg tcttgggggc gatgacgcgg atctcggggc agccgagcgt
     3901 ggtgagcgcg tggatctgcg agcgcgccac ccgcgaatgc aggacatcgc cgacgatcgc
     3961 cacccgcagg ttcgagaagt cccccttgta gtggcggatg gtgtacatgt cgagcaggcc
     4021 ctgcgtcggg tgcgaatgac gcccgtcgcc ggcgttgatg acgtgaatgt cgggcgcgac
     4081 gtgacgcgcg atgaagtgcg gcgcgccgct ctcgccgtgc cgcaccacga acatgtcggc
     4141 gtgcatcgcc gacaggttgt ccaccgtgtc gagcaggctc tcgcccttgg actgcgagga
     4201 cacggccacg ttgaggttga tgacgtcggc cgaaaggcgc ttcgcggcga tctcgaaagt
     4261 ggtgcgggtg cgcgtggaag gctcgaagaa gacgttgaat accgacttgc cgcgcagcag
     4321 cggcagcttc ttcaccttga cgccgacgaa cgttttcgcc gtatcgagga tctgccagag
     4381 gatctcggtg ggcagcccct cgagcgtgag caggtggtgc aactgccctt tcttgttgag
     4441 ctgggggttg gaggggtgga gccacattct atttggcctc ctcgcgcagc tcgagggtga
     4501 gccgcccctt ggcgtcgcgc ttgagcccga tgctctggcc gggcttgagc gcgagcgtgg
     4561 cgccggcaaa ctgcgcggcg atcggcagtt gccgtccgcc gcggtccacc agcgtggcga
     4621 gcctcacggc tgccggccgc ccgtagtcga aaatctcgtt cagcgccgcg cgtatggtgc
     4681 ggccggtgtt gagcacgtcg tcgaccagga tgaggtcacg gccttcgacc tcgaacggta
     4741 tctccgagcg cttgacgtcg cggtgcagcc cacgggcctc gtagtcgtcg cggtagaagg
     4801 aaacgtcgag ggtggcgagc ggcgtctgga gcttcaacgc ctggtgcagc ctttcggcga
     4861 cccagacgcc gccggtatgg atgcctatca aacccgtttc cggccccacc gcgggccgca
     4921 tctgctcgat cagcgctttc agcagctgct cggcgtcagg taacgggttg ccgggcatgt
     4981 gtaatcgttc ttgcggcgcg ctagggtatc acaattcgcc gctccctggt gagcggcggc
     5041 gagagctggt gactaagtga ctaggtgatt aagtgattga gcggcgagag ccggaaggcc
     5101 gactcggggt tacctaatca ctcagttact ctgacctatt tcgccgtggc gaaataggtc
     5161 tccagtatgc gctgcgccgc gacggcgtcg aggccggcct tcagccgcgc gccgcgcgcg
     5221 cccgcggcgc gcagcattcc ctcggcctcg tgcgaggtga gccgttcgtc caccagctcg
     5281 gtgcgtatgc cgaagcggcc ttgcagccgc tgcgcgaaac ggcgggcgag ccggccgacc
     5341 gggtgctcgg cgccatcggg atgcgcgggg agtccgacca ccagcaacgc cggccgccat
     5401 tccacgatga gccgctcgat tgccgcaaac cgggcgcggt tttccgcggc cgacaccgtg
     5461 gtaaggggat gagcgagccg ggtttcgaag tcgccgaccg cgacgccgat gcgctgggtg
     5521 ccgaaatcga aagcgagaac ggtgcccgtc ctggggcgag gcgtgaggtg tgaggcgtga
     5581 ggcgttttca gaatggagcg tcgaggttgg tcaaggccca ggtcttccgc ctaacgcctt
     5641 acgcctgact cctcaccgcc gttaggcgtg tcccgcctgc tcggagaggc tcgcgaaatc
     5701 gatgccgagc agccccatgg cggcgggcag ccgctcttcc gcccgcaaat cgaagatcac
     5761 ctccggcctg gcctggacgg taagccacgc gttctgcgtg agctcgtgct cgagctgacc
     5821 gggcgcccag ccggcatagc cgagcgtcac caggagcttc cccggcccct cgccccgcgc
     5881 caccgcctgc aggatgtcct tggaggtggt gaggccgatc tcggaactca cggcgagggt
     5941 ggactgccac tggccgagcg gcgtgtgcag cacgaacccg cggtccacct ggaccgggcc
     6001 gccgaaatgg atcgggatcg acttgaaagc ctcgctcgcg agcgggatgc tcacctgttc
     6061 gagcagcgcg tgcagggtca tctcgatcgg gcggttcacc acgatgccga gcgcgccctg
     6121 atcgttgtgc tcgcagatgt aggtgagcgt tttggtaaag tgcgggtccg ccatgttggg
     6181 catggcgatg aggaagtgct gtgtcaggtt catcttttgc atggcttccg ctgtttcccg
     6241 ccttccgctc ccgcgcgttg aacggcgctc aatgcgtcca ttcgaacgcg cgggcttaaa
     6301 ccggagcttt gcttccggtt caaggtttaa ttgtaaatgc cgcggccccc cttcacaatt
     6361 gcgcgcgacc cggccccgct gaaccgttac gcatcaagcc cctgccgatt ccgccgcgct
     6421 ctacgccgtc ctcgatgcga gctggcgggt ccattgcgct ttcggctttg acacggaaat
     6481 cccggacaag ctcccttcgc gccgcgaccg cgggatcggg ggcagccacc cgagcgtgac
     6541 gcttcagccc tcctgcacgt aagtgccggg cgcgtcggca agcttgggat aacgatcggt
     6601 cgccgtgggc gggagcttga ctcgaccgcc acagcgcgcc gcgagccatt gcacccagtc
     6661 ttcccaccac gtgccgtgga tttccttctg ctccttgagc cagtcctcgg gcggggcgtc
     6721 ggtggcggcg ccggtccagt aactgcgctt gggcggcttc accggggggt tgatgatgcc
     6781 gagaatgtgg ccagagctgg tcagcgtgta gcgggagggc gcgctgatcg tcgacgtgtt
     6841 cttgaacgtg gccttccacg gcgcgatgtg atcttcctcg cagcccaccg cgtagagcgg
     6901 ctggcggata agcgcaaggt cgatcgggtg ccccgcgagc gtgacgccat ccttgcggat
     6961 gagccggttg tgcaggtaaa actcccgcag gtagaacgag tgcatcgcgc gcgccatgcg
     7021 ggtgacgtcg gtgttccagt agagcacgtc gaacgcgggc ggcgtctcgc cgtagagata
     7081 gccgtgcacg aaatagtgcc agatgagggt gttgggacgc agcatccgga acgtccgtcc
     7141 catctcgcgg ccgtcgaggt agccctggcg ggccatcatg tcctcgagca cgctgatcgt
     7201 ggactcgtcg atgaacacct cgaccgcccc cgggcgcgag aagtcgagca gcgtggtgag
     7261 cagcgtccag tgcgcgaccg gcacgtcgtc agggttcgcg tgttcgcggc tgtaccacgc
     7321 catcatcgcg gcgagcgcgg tgccgccgat gcagtagccg gctgcatgca cgcgcggctg
     7381 gccgctcacg gcgcgggcca cgtccaccgc gcggcggatg ccgtccatga ggtaggcgtc
     7441 gaaggtggtc tccgccatgt cggcggtcgg attcttccag ctcaccatgt agacgtcgaa
     7501 gccctggttc acgaggtgcc ggaccaggct tttcttttcg gtgaggtcca agatgtagta
     7561 cttgttgatc cacggcggca cgaacacgac cggcagcgcg tgcacctcgc cggtcgcggg
     7621 cgcgtagtgg atgagctcca tcagggaatt gcggtacacg accgcgccgg gcgtggtcgc
     7681 gaggttcccg ccgaccttga acgccgagcg atccaccatc tgaatgtcgc cgacgcgcac
     7741 atcgtcgagc cagttcttca gcccccgcac caggctctct ccgccggtct cccagaactt
     7801 gcgcagcgcc accgggttgg tgagcaggta attgccggga gcgaccgcat cgagccactg
     7861 gcgcgcccag aacgcggcgc ggcggcgctc cctagccgag gcgccgggcg tatcgtagag
     7921 cgcgtcctcg agccagcgcg tgtagagcag gtagtactgc ttgagcagcg agaacgtcgg
     7981 cacctccgac caggcgggat cggcgaagcg ttcgtcgttg tgagtggcca ccgccggacg
     8041 ctgcggctcg agcccggcgg cgacctgcca ggcgtgaagc tgcaggcggt gcagttccac
     8101 ggcgagcgtg gacagcgtct cggagagctc ccgcgggtgg ccgagccatt cccgggcgac
     8161 cttgttgaac gaggcagcga tgtcgaaagg atccgttgtg tcgttcagcg cccggacctg
     8221 ctcgctccag gcctgcagac cggcaagcgg ggcttcgggt tcggcggtcc tggaacgctt
     8281 cctcatggcg ggtatcctac ttgcgccaga aggcgggggt aaagatcacc agtacggtga
     8341 agagttcgag ccgcccgatc agcatcgcga tcgcgcagac ccaggtctgg aaatcgctaa
     8401 gcactgcgaa cgtcgtggcg gggccaacct tgtcgagccc cgggccggtg ttggtgatgc
     8461 aggcgatgac tgcggtgaag gcgctcacga agtcgaggcc ggagacgagc agcagcagcg
     8521 tcatcacggt gacgctcgca ccccacagcg tcatgaaggc gagcaccgca aatacgatct
     8581 ggttcgccac cacctggccg ccgagcttga cgggcacttg ggcctgagga tgaaggagct
     8641 tcaccatctc ccgcagcccc tgcaggaaca gcaactggaa acgcatcatc ttgatgccgc
     8701 cgccagtgga gcccgcgcag gacgcgatgc agctcagcat tagcatgacc atcggcgcga
     8761 aaaccggcca ctggttgaag tccacgctgg cgtagccggt cgtagtggct agggaaaccg
     8821 tgttgaacgt cgcgcgacgc agggcggtga ggaaatccgg ataaacgcct ttttgccaca
     8881 ggaataccgc aatcaaggcg caactgccca gaacgattat cagaagccag cgaacctctg
     8941 gatcgagacg atacggctcg aagctgcgct tgcgcagtgc aaggaaatgg gtggcgaaat
     9001 tcatgcccgc gaccagcatg aagatggtcg agaccgcctc gatcagcggg gagtcgaaaa
     9061 atccaaagct cgcgtcgtgg gtcgagaacc cgcccaggct catggtggtg aaactgtgca
     9121 tcacggcgtc gagcggcgtc atgccggccg cccagtagct gaagatgcag gcgagagtga
     9181 tgagcgagta gacgaaccag agccccttgg cggtctcggt gatgcgcggc gtcagcttcg
     9241 tgtccttcat cggtcccggc gtctcggcac ggaagacctg ccggccgccg acgccgagca
     9301 gcggcaggat cgccaccgcc agcacgatga cgcccatgcc gccgagccat tgcaggaagc
     9361 cgcgccagat gttgatggag ggcggcaact ggtcgagatc ggacaggacc gtcgcgccgg
     9421 tcgtggtcat ccccgacatg cattcgaagt aggcgtcggt gaagctcaat ccaggcagga
     9481 agaacatcag cggcagcgcg gagaacgccg gcagcaatgc ccagaccagc gtcaccagca
     9541 ggaagccgtc gcgcacctgc aactcacgcc gcgcttggcg cgtcgcgatc caaagcactg
     9601 cgccgcagac gagcgtgatc agcaccgact ggtcgtaggc atatagcgcg gcgtcgtgat
     9661 agaagaacgc caccgccagc ggcaccaaca tggtgctgct gaaaatcatg atgaccatgg
     9721 cgagcacgtg cagtaccgcg agcacgctct gcgccggccg cgcctgctct agggtgaggg
     9781 tatccgccac gtcgttgcgg gtggttgcgg cagcagcgaa gccgtcagaa gaagccgaca
     9841 tccacctgga acagcttctc gatcttcggc actatgcgct tgttcacgac gaacacgatg
     9901 acgtgatcgt cggactcgat gatcgtgtcg tggtgggcga tgatgacgtc ggagtcatcg
     9961 ccgttgcgct cggccggtgt tccggagcgg tcgcggcggc gcacgatcgc gccgatggtt
    10021 acgcctttgg gaagatcgag ctcttcgatt cggcggccga caaccttgga ggacttccgg
    10081 tcgccatgcg cgatcagctc gagggcttcc gccgcgccgc ggcgcaacga gtgcaccacg
    10141 gcgcagtctc cacgccgcac gtgcgcgagc agcgtgccga tggtggcttg cgccggcgag
    10201 atcgcgatgt cgatctggcc ggactggacg aggttcacgt aggagctgcg gttgatgagc
    10261 gagatcacct tgcgcgcgcc ccggcgcttg gccagcagcg agctcatgat gttggtctcg
    10321 tcgtcgttgg tgaccgcgca gtagacgtcc atctcggaca cgttctccgc gtcgagcagc
    10381 tcctcgtcgg tgacgtcgcc cgaaagcacc agcgtcttgt cgagctcggc ggcgagccgc
    10441 tcggcccccg tcttgttgaa ctcgatgatc ttgacctcgt acttgtcctc gatcgcctgc
    10501 gcgaggcgcc ggccgatgtt gccgccgccg ccgaccatca cgcgcttcac cggccggtcc
    10561 atgcggcgca gctcgcgcaa cacgttgcgg atgttcccgg tggcggcgat gaaaaacacc
    10621 tcgtcgccgg cttcgaccac cgtccggccc tcgggcgcga tcggactgtc ctggcggaag
    10681 atcgcggcca cgcgcgtatc gacctgcggc atgtgctggc gcaggtgctg gatctcgtgc
    10741 ccgaccagcg gcccgccgtg gaacgcgcgc accgcggcga ggcttacctt gccgtcggcg
    10801 aactcgagca cctgcagcga ttcggggaat tcgagcagct tcacgatgta atcgcacagg
    10861 atctgctcgg gacagatcga atggtccacc gcgaagttct cgggcgagaa gatctcggga
    10921 tgcgaaagat agtcggcgga acggatgcgc gcgatcttgg tcggaatgtt gaacatggtg
    10981 gcggcaagct tgcacgccac catgttggtc tcgtcgcttt gcgtcaccgc gagcagcatg
    11041 tcggcgtcgc gcgcacccgc ctcctccagc accgcggggt gggcggcgtt gccggtgacg
    11101 gtccgcagat cgagacggtc ctgaaggcgc ttcagccgcc ccggctcgat gtcgaccacg
    11161 gtaatatcat tggcctcgga ggccaggctt tcggcgaccg tcgagccgac ctggccagcc
    11221 cccaggatca cgatcttcac gcgggtcctt ccgcaaaaag cgcgattcta gccccctttg
    11281 cccccgcgcg gcagcgcgtg tcgatgagcg atcgggcagg cggcgcgtac agcatggcat
    11341 aatttcgctc ccgaacgcgg gggcaagaac ttgacgcgca tatatttttc cggcgccatc
    11401 acggccggcg cgcggtgcga actgccgccc gtccaggcgc gtcacgtcgc gcgggtgctg
    11461 cggttgcgca ccggggatgc ggtcacgctc ttcaacggcg acggcgcgga atacgcggct
    11521 gtcgtggagc gcgttgcgaa ggggggcgtg acggcgaaag tgaccgggcg ccgtctcgtc
    11581 gagcgcgaat cccggctcga aatcgtgctc ggccaggcgc tctccagcgg cgagcgcatg
    11641 gactatacgg tgcagaaagc ggtcgagctg ggggtctccg ccgtccaccc ccttgccgcg
    11701 gcccgcagcg tcgtgcgcct gagcgcggag cgggcgggga agcgcgtttc gcactggcag
    11761 gcagtggcga tcgcggcatg cgagcaatgc ggccgcaacc gcgttccgcc ggtcgcaccg
    11821 gtcgtggcgc tcgatgaatg gctctcacgc cacaacgcca atcacgatgc gatgctgtct
    11881 gtattgctgt cgccagaggc cgaaacccgg ctgcgcgacc ttcccaaacc ggacggcgcc
    11941 atcgtgctgc tggcggggcc ggaaggcggc ttcaaccccg aggaggaatc cctggcgcgg
    12001 gcgagcggct tcacgccggt taggctgggt ccgcgcgtgc tgcgcaccga gaccgccgcg
    12061 atcgcagcgc tcgccgcgat gcaagcgctg tggggggact tctgaaggca gaaggc
//
LOCUS       DBNGWZ010000466         7794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_188588, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000466 DBNGWZ010000000
VERSION     DBNGWZ010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_188588"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1387)
                     /locus_tag="AC16CX_14305"
     CDS             complement(20..1387)
                     /locus_tag="AC16CX_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3013715.1"
                     /translation="MKPDPGWECSTAADARYQSIYVPFDNSAHALRGLDLAVAIARET
                     GACITGSHVYAAGLHDRRFRQMESGLPERYQEEEKLAEQREVHDDLITRGLGLISECY
                     LDVVSRQCAEAHVPYRRVMLEGKNWQRLVEDIHASSHDLVIMGALGLGAVASSAVGSV
                     CERVARRIDRDLLVVRAAGGKPPGAIVVGVDGSTHAFGALKSALELARTFERPVEAVA
                     VYDPFFHYIAFHGIAGVLSPAAASVFRFREQERLHEEIIDAGLARIYQSHLEVAAKIA
                     AAEGVELHSRLLAGKAFEQLLNYVREREPWLLAVGRIGAHSDATMDLGSTTENLLRAA
                     PCNLLLAARTFAPTVEQVADVTMTWTREAEARMQRVPERARGMARRAVVMHAAAHGHT
                     MVTSDVIDACLAAFARTGAAHAAPGKTLQEGTAKAGKCPFAHRANEGRRKPRRTEPIA
                     DQGRK"
     gene            complement(1359..1640)
                     /locus_tag="AC16CX_14310"
     CDS             complement(1359..1640)
                     /locus_tag="AC16CX_14310"
                     /inference="COORDINATES: protein motif:HMM:NF019968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HIR3013716.1"
                     /translation="MALLTNPQETRLVRSLGLTIGGRTTLSRSPEWMPGALTEAKLLW
                     TEEAERRLERVPEVVRPMVRDSIERHARERGCREITCEVMDEARSRMGM"
     gene            complement(1765..2202)
                     /locus_tag="AC16CX_14315"
     CDS             complement(1765..2202)
                     /locus_tag="AC16CX_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013717.1"
                     /translation="MPRAVEHRELQRAANRDIAWAAVWAGFAIALGGTLAAAAWAFAF
                     AGKGTWWAANLGTLSLFCGALFAGWRAGTVEPLNGAYIAVFYFSAAAAVIFGGEFLGV
                     LPDPLPGLPRGDSTFFFVWPLAQLAAGTIGAIVGGVLARRRST"
     gene            complement(2186..2980)
                     /locus_tag="AC16CX_14320"
     CDS             complement(2186..2980)
                     /locus_tag="AC16CX_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013718.1"
                     /translation="MTDAVDGRTAPTSQRNTAAPMPTWRERGQRVRSPAIFAACALLV
                     IWGGLPWLPAAWGVRFGAPYLTFFSLAAVGAACFFMLLNWGPIRQPRSALATFASILL
                     VYVVTVGGMVAFGYWYYPQFETPATRTTGPAAQVSAAEQRGRAVFLNPQHACFACHTI
                     EALGIRGGSRGPDLSSVGRQAEGRKRGVPAEDYLRESIVDPWACFTPLPASGLIECQA
                     AADPAKTYPQLMPPGAKERITAQELGDLIAFLKSLRGAGERNAKGG"
     gene            complement(2973..4382)
                     /locus_tag="AC16CX_14325"
     CDS             complement(2973..4382)
                     /locus_tag="AC16CX_14325"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3013719.1"
                     /translation="MPLDFPLIGARTAIWLVAQLHLFFAAFVLGAPIFIVVSEWLGLR
                     TGDPRYERLAKETMKVVAIAYSLTAIFGVLFAFVLMGPYQPVTNYLFRALYPVWVAYG
                     AFILLETVCMYLYWYTWEPLSRSAGGKWTHIGIGVLLNVLGTVVMLLMNAVTGFMNTP
                     PKALESASLWAMIDNAAWWPLNLHRFLGNVTFGGFVVALFAAFMFLTSRKDEDWAFYD
                     WMGYIGNLIGVGALITMPLAGYILSKELFVYDAQLATFMMADKLSGYFVMQGLLVVLL
                     FLGANFYIWLSMQRIEDGARFAPHMKVVFGVLLAGGIVWVVPQNFFPDLLAKAPAGMS
                     EQALILPERLTFLGLMVAKALAVTAIIIMTFVTYLLYRRARATGTIRWGRIDPMAQYV
                     LIFIPAVAVYLMGLMGAIRSFTRMDYHIYGAVKDVTPYWYTATLGHSSIVVAVTTLLF
                     FMLVAFVFWVGFVVGKAHD"
     gene            complement(4384..5043)
                     /locus_tag="AC16CX_14330"
     CDS             complement(4384..5043)
                     /locus_tag="AC16CX_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013720.1"
                     /translation="MAGAFVTQGERQTTLLKRTAVTACAATVLLGTLVLMGWMLARVA
                     EHPGVPREISELPSWFTSAMPVPGARQRSPASAAAAGEAAAAHRYEAGTTIASVKPGA
                     AAARPPAKAAPAEGNYTDFASGQVEIALKEYEFVPGRIRTRPGTVTFVLRNEGRFAHD
                     FHVEGPGVDTHADKFSPGRTIRVQVTMQEGEYKVSCPLSNHDERGMHATLIVTSKLAG
                     G"
     gene            complement(5057..5701)
                     /locus_tag="AC16CX_14335"
     CDS             complement(5057..5701)
                     /locus_tag="AC16CX_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013721.1"
                     /translation="MFKQKLLVVVLGAAIAALCGTAAAQTAPKRTAGLSPNCAANAPV
                     RDYLVTVGEWDTVPKGHDGKPVAYNDRGFARDANSKLERYNFDPSVIIVKRGDCVNLR
                     IHDVKGSHHNVMIEGTEIGSEGAPLIDDAGKVIGKAAARANPNAFEGPDKLKAGQFVR
                     GEQVMVKFQANQPGTYRMICDVHTFIGPNGELRGYDDKGKPVQGPMIAYITVLP"
     gene            5874..7283
                     /locus_tag="AC16CX_14340"
     CDS             5874..7283
                     /locus_tag="AC16CX_14340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013722.1"
                     /translation="MDLLARFSLVRLLLAGGAVILLAGMLVVGTWVEREIESGVTSRA
                     ALVTSLYVDSLVSANLQSLAGNGELSEADRAALGRLLSGTPLGQHIVAFKVWALDGRI
                     LYSTNPALTGRRFPVGKALASALAGHVYSRIADLTEPENEFERQQWPRLIETYAPVRA
                     ANTGTIIAVSEFYQTTDELAQEVRAARLRSWLMVGTSTLVMFLLLAWLVRRASNTITA
                     QQSELSEKVTQLTTLLAQNEQLHERVRRAAARTTALNERFLHRIAADLHDGPGQSLAL
                     ALMRMETLADACSACPATIARERTVGEEFRTLHAALQSALEDLRAISGGLHLPEIERL
                     SVADTARRAVHDYERKAGLAIALSVDDVPPEAPLPVKITLFRLLQESLANGFRHGGGA
                     DQRVTVNVRDGHLLVEVADGGAGFDLQIAAAEGHLGLAGMRERVEILGGTFGVQSAPG
                     RGTVIRASLPMAPLETGRE"
     gene            7276..>7794
                     /locus_tag="AC16CX_14345"
     CDS             7276..>7794
                     /locus_tag="AC16CX_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013723.1"
                     /translation="MSDPVRILVADDHPLFRDGVVHSLASEPDFAIVGQAASGEEALR
                     LARDFLPDVVLLDIGMPGWDGLVTAEKITTACPATKIVMLTVAEDEDKLLAAFKAGAR
                     GYVLKGVSARELANVVRAAAQGEVYVSPSLAAGILVAHTRARPADPLEELTERELEIL
                     KLVGQGLTNREIG"
BASE COUNT         1356 a         2682 c         2489 g         1267 t
ORIGIN      
        1 tgatccatgg ctggcagtgt catttccgtc cttgatccgc gatcggctcc gtccggcgcg
       61 gcttgcgcct gccctcgttg gcgcgatgcg cgaacgggca tttccccgct ttcgccgtcc
      121 cttcctgcag ggtctttcca ggcgccgcgt gcgcggcacc ggtccgcgca aaggcggcga
      181 ggcaggcgtc gatcacgtcg gaagtgacca tggtatgtcc gtgagcggcg gcatgcatca
      241 cgaccgcgcg ccgcgccatg ccgcgcgcgc gttctggcac gcgttgcatg cgcgcttccg
      301 cctcgcgcgt ccaggtcatg gttacgtccg cgacttgctc caccgtcggc gcaaacgtgc
      361 gcgccgcgag tagcaggttg cacggcgcag cgcgcagcag gttctcggtt gtactgccga
      421 ggtccatcgt ggcatccgag tgggcgccga tgcgaccgac ggcgagcagc cacggttcgc
      481 gctcacgcac gtagttcagg agctgctcga aggccttgcc ggcgaggagc cggctgtgca
      541 gttccacgcc ttccgcggcc gcgatctttg ccgcaacctc gagatgcgac tggtagatcc
      601 tcgcgagacc ggcgtcgatg atctcctcgt gcagtctttc ctgctcccgg aaacgaaaga
      661 cacttgcggc ggctggagag agcaccccgg cgatgccgtg aaaggcaatg tagtggaaga
      721 acggatcgta gacggccacc gcctccaccg gccgttcgaa ggtccgcgcc aactcgagtg
      781 ccgacttgag cgcgccgaac gcatgcgtgc tgccgtccac gcccaccacg attgcgcccg
      841 gcggcttccc gcccgctgcc ctgacgacga gcagatcgcg gtcaatgcgc cgcgctaccc
      901 gctcgcaaac gctacctacc gccgacgacg cgaccgctcc gagccccagt gcgcccatga
      961 tcacgagatc gtggctcgaa gcgtggatgt cctccacgag cctttgccag ttcttgccct
     1021 ccagcatcac gcgccggtac ggcacgtgcg cttccgcgca ttgtctgctg acgacatcga
     1081 gatagcactc ggagatcagg ccgagtccgc gggtgatcag atcgtcatga acctcgcgct
     1141 gctcggcaag cttctcctcc tcttggtagc gctccggcag gccgctttcc atctggcgga
     1201 agcgccggtc gtgcaagccc gcggcgtaga cgtggcttcc cgtgatacag gcgccggttt
     1261 ccctggcgat ggcgacggcg agatcaaggc ctcgcaacgc atgcgccgaa ttgtcgaagg
     1321 gaacgtagat cgactggtag cgcgcgtcgg cagcggtgct acattcccat cctggatctg
     1381 gcttcatcca tcacctcgca agtgatttcg cggcagcccc gctcccgcgc gtggcgctca
     1441 atgctgtcgc gcaccatcgg cctcacgacc tcgggaacgc gctcaagccg ccgctccgcc
     1501 tcctcggtcc acagcagctt cgcctccgta agcgcacccg gcatccattc cggcgaccgg
     1561 gagagcgtgg tccgccctcc gatcgtcagc cccaggctgc gcaccagccg tgtctcctgg
     1621 gggttggtca gcagtgccac gcgtcgttcg cagcgcggac agcgaaagat cgcggtgagc
     1681 gaatctccac tcggagtgtc cgccgcttcg atcaacttca tcgctgcatc acacgccacg
     1741 cacaggaacc tcatcggcag ccccctacgt gctccgtcgc cgcgccaaga ccccgcccac
     1801 gatcgcgcca atcgtgcccg cggcgagttg cgcgagcggc cacacgaaga aaaacgtcga
     1861 atccccgcgc ggcaggcccg gcagcggatc cggcaggact ccgagaaatt cgccgccgaa
     1921 aatcacagcc gccgcggcgc tgaagtagaa caccgcgatg tacgctccgt tcagcggctc
     1981 caccgtcccg gcccgccacc ctgcaaacag agcgccgcag aacaaggaca gggtgcccag
     2041 attggcggcc caccacgttc cctttccggc gaacgcaaag gcccaggccg ccgcagccag
     2101 cgtgccgccg agcgcgatcg caaagcccgc ccacaccgcg gcccaagcga tgtcacggtt
     2161 tgccgcgcgt tgcagttccc gatgctcaac cgcccttggc attgcgctca cccgcgcctc
     2221 gcaggctttt cagaaaggcg atgagatcgc cgagctcctg cgccgtgatc cgctccttgg
     2281 cccccggcgg catcagctgg ggataggtct tcgccggatc cgccgccgcc tggcattcga
     2341 tgagcccgct cgcgggcagg ggcgtgaagc acgcccaagg gtccacgatc gactcgcgca
     2401 gatagtcctc ggccggtacg ccccgcttgc gtccctcggc ttgcctgcca acggaagaca
     2461 gatcgggacc gcggctaccg ccgcggatgc cgagcgcttc gatcgtatgg caggcaaagc
     2521 acgcgtgctg gggattcaag aacaccgccc tcccgcgctg ttccgcggcg ctcacctgcg
     2581 ctgccggtcc ggtcgtcctt gtcgcgggcg tttcgaactg ggggtagtac caatagccga
     2641 acgccaccat gccgcccacc gtgacaacgt agacgagcag gatgctcgcg aacgtggcga
     2701 gcgccgaccg gggctggcgg atcgggcccc agttaagcag catgaaaaag caggccgccc
     2761 cgaccgcggc gaggctgaag aacgtgagat agggcgcgcc aaagcgcacg ccccacgccg
     2821 ccggcaacca cggcaaaccg ccccagatca cgagcagtgc acacgccgcg aatatcgccg
     2881 gacttctcac gcgctgcccg cgctctctcc acgtcggcat gggagcagcg gtgttgcgct
     2941 ggcttgtggg agcggtgcgg ccgtccactg catcagtcat gagctttccc cacgacgaag
     3001 ccgacccaga agacgaacgc caccagcatg aagaacagca gcgtggtgac ggccaccacg
     3061 atcgacgaat ggcccagcgt cgccgtgtac cagtacggcg tgacgtcctt gaccgcgccg
     3121 tagatgtggt agtccatgcg cgtgaacgag cgaatcgccc ccatcagccc catcaggtag
     3181 accgccacgg cgggaatgaa gatgagcacg tactgcgcca tcggatcgat gcggccccac
     3241 cggatcgtgc cggtcgcgcg cgcccgccgg tagagcaggt aggtgacgaa ggtcatgatg
     3301 atgatcgcgg tcacggcgag cgccttcgcc accatcaggc cgagaaacgt gaggcgttcc
     3361 ggcaggatca gcgcctgctc gctcatcccg gcgggagcct tcgccagcag atcggggaag
     3421 aaattctgcg gcaccaccca gacgatgccg cccgcgagca gcacgccgaa gaccaccttc
     3481 atgtgcggcg cgaagcgcgc gccgtcctcg atgcgctgca tggaaagcca gatgtagaag
     3541 ttcgcgccga gaaagagcag caccaccagc agcccctgca tcacgaaata acctgagagc
     3601 ttgtccgcca tcatgaaggt ggcgagctgc gcgtcgtaga cgaacagctc cttggagagg
     3661 atgtagccgg cgagcggcat cgtgatcagc gcgcccacgc cgatcaggtt gccgatgtag
     3721 cccatccagt cgtagaacgc ccagtcctcg tccttcctcg aggtgaggaa catgaaagcc
     3781 gcgaacagcg cgaccacgaa gccgccgaag gtgacgttgc cgagaaagcg gtgcaggttg
     3841 agcggccacc acgcggcgtt gtcgatcatc gcccacaggc tggcgctctc gagcgccttg
     3901 ggcggcgtgt tcatgaaacc ggtcaccgcg ttcatcagca gcatgacgac ggtgccgagc
     3961 acgttgagca gcaccccgat gccgatatgc gtccatttgc cgcccgccga gcggctgaga
     4021 ggctcccagg tgtaccagta gaggtacatg cacacggttt cgagcaggat gaacgcccca
     4081 taggcgaccc acaccgggta gagcgccctg aacaggtagt tggtgaccgg ctggtacggg
     4141 cccatcagca cgaacgcgaa cagcaccccg aagatcgcgg tgagcgagta ggcgatcgcc
     4201 accaccttca tcgtctcttt cgccaggcgc tcgtatctcg ggtcgcctgt gcgcagcccc
     4261 agccattcgc tgaccacgat gaagatcggc gcgcccagca caaaagcggc aaagaacaga
     4321 tgaagctgcg ccaccagcca tattgccgtg cgcgcgccga ttagcggaaa atcgagcggc
     4381 acgtcagccc ccggcaagct tcgaggtcac gatcagcgtg gcgtgcatgc cgcgctcgtc
     4441 gtgattggaa agcgggcagc tcaccttgta ctcgccttcc tgcatcgtga cctgcacgcg
     4501 gatggtgcgg cccgggctga acttgtcggc atgggtgtcg acccccggac cctcgacatg
     4561 gaagtcgtgc gcgaaacggc cctcgttgcg caacacgaag gtgaccgtgc ccggccgcgt
     4621 gcggatcctg cccggcacga attcgtattc cttgagcgcg atctcgacct ggccgcttgc
     4681 gaaatccgtg taattgccct ctgctggggc ggcctttgcc ggcggccgcg ccgcggccgc
     4741 accgggcttg accgaggcaa ttgtcgtgcc cgcctcatac cggtgcgcgg cggcggcctc
     4801 cccagccgcc gccgctgacg caggagaccg ctgccgcgca ccgggcaccg gcatcgcgct
     4861 ggtgaaccag gagggcaact cacttatttc gcgcggcacc cccggatgct cggccacgcg
     4921 ggcaagcatc cagcccatca gcaccagcgt gcccaggaga accgtcgccg cgcaggcggt
     4981 tacggcggtt ctcttcagga gtgttgtctg ccgttctccc tgagtgacga aagcgcccgc
     5041 cacactgcgc cgcccgtcac ggcagcacgg tgatgtaggc gatcatcggg ccctgcaccg
     5101 gcttgccctt gtcgtcgtag ccccgcaact ctccgttcgg cccgatgaag gtgtgcacgt
     5161 cgcagatcat gcggtaggta ccgggctggt tggcctggaa cttcaccatg acttgctcgc
     5221 cccgcacgaa ctggccggcc ttcaatttgt ccggaccttc gaacgcgttg gggttcgccc
     5281 gggcggccgc cttgccgatc actttgcccg cgtcatcgat gagcggggct ccctcggagc
     5341 caatctcggt tccctcgatc attacgttgt ggtgagatcc cttcacgtcg tgtatccgca
     5401 ggttcacgca gtcgccccgc ttcacgatga tgaccgaagg atcgaagttg taacgctcga
     5461 gctttgagtt cgcgtcacgg gcgaaccccc ggtcgttgta cgcaacgggt ttcccgtcat
     5521 gacccttggg cacggtgtcc cattcgccga cggtgacgag gtagtccctg accggcgcat
     5581 tggccgcgca gttcggcgac aatccagcgg tgcgcttcgg tgcggtctgc gccgcggccg
     5641 tgccgcatag cgccgcgatc gcggcgccga gtactacaac caataacttt tgcttgaaca
     5701 tgctgctctc ctttcagaaa agtcagtctc ctgacctccg tctatcgtgc aaaaaacaag
     5761 gcgggagaac aacggctgat gggcgccaat gtgtcgggac cttgggccct ccggcgcggg
     5821 ccattggtcc atagggtgca tccaaccctg cctgctacaa tgaattcgcc gccatggacc
     5881 ttctcgcccg gttcagcctc gtgcgcctgc tccttgccgg aggcgccgta atcttgctgg
     5941 ccgggatgct ggtcgtcggc acctgggtgg aacgcgagat cgaaagcggg gtcaccagcc
     6001 gcgcggccct ggtgaccagc ctctacgtgg acagtctcgt ttccgccaat cttcagtcgc
     6061 tcgccggcaa cggcgagctg agcgaggcgg accgcgcggc gctggggcgt ctgctctccg
     6121 gcacgccgct cggacagcac atcgtggcat tcaaggtgtg ggccctcgat gggcgcatcc
     6181 tctacagcac caatccggcc ctcaccggac ggcggtttcc ggtagggaag gcgctggcct
     6241 ccgcgcttgc cggccacgtg tactcccgga tcgccgatct caccgagccg gaaaacgagt
     6301 tcgagcggca acagtggccg cgcctgatcg agacctatgc gccggtgcgc gcggcgaaca
     6361 cgggcacgat catcgcggtc tcggagttct accaaacgac cgatgaactg gcgcaggagg
     6421 tgcgcgccgc gcggctgcgc agctggctca tggtaggcac gtcgacgctg gtgatgtttc
     6481 tgctgctcgc ctggctcgtg aggcgtgcaa gcaacaccat caccgcgcag cagagcgagc
     6541 tgagcgagaa ggtgacacaa ctcaccactt tgctcgccca gaacgagcag ctgcacgagc
     6601 gggtgcggcg cgccgccgcg cgcaccacgg cgctcaacga gcgattcctg caccgcattg
     6661 ccgccgacct ccatgacggc ccgggccaaa gcctggcgct cgcgctgatg cgcatggaaa
     6721 cgttggccga cgcctgcagc gcctgccccg caaccatcgc cagggagcgc acggtgggcg
     6781 aggaattccg aacgctccat gccgcgctgc agtccgcgct cgaggatctg cgcgccatct
     6841 ccggcgggct gcatctgccc gagatcgagc ggctctcggt cgccgacacc gcgcggcgcg
     6901 cggtgcacga ttacgagcgt aaggcaggcc tggcgatcgc attgagcgtg gacgacgtgc
     6961 cgccggaggc gccgctgccg gtcaagatca ccttgtttcg cctgctgcag gagtcactcg
     7021 ccaacggctt ccgccatggc ggaggagcag atcagcgagt caccgtgaac gtgcgtgacg
     7081 ggcacctgtt ggtcgaggtc gccgatggcg gagcgggttt cgatctgcag atcgcggccg
     7141 cggaaggcca cctggggctc gccggcatgc gcgagcgggt ggaaattctc ggtgggacgt
     7201 tcggcgtgca aagcgccccc gggaggggaa ccgtgatccg ggcatcactg cccatggcgc
     7261 cgctggagac ggggcgtgag tgatccggtc cggatcctgg tcgcggacga tcacccgctg
     7321 ttccgggacg gcgtcgtgca ctcgctcgcc tcggaaccgg atttcgccat cgtcggccag
     7381 gccgccagcg gcgaggaggc gctgcgcctg gcgcgcgact ttctgcccga tgtggtgctg
     7441 ctcgacatcg gcatgcccgg ctgggacggg ctggtcaccg ctgaaaaaat caccaccgcc
     7501 tgtcccgcga ccaagatcgt catgctgacc gtcgccgagg atgaagacaa actgctggct
     7561 gcgttcaagg ccggagcgcg cggctacgtg ctcaaggggg tgtccgcacg cgagctggcc
     7621 aacgtggtcc gcgccgccgc ccaaggggaa gtttacgtgt cgccgtcact cgcggcgggc
     7681 atactggtcg cacacacgcg tgcccgaccg gccgatccgc tcgaagaact gaccgaacgg
     7741 gagctcgaaa tcctgaagct cgtgggacaa gggctcacca accgcgagat cggt
//
LOCUS       DBNGWZ010000467         5886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2579704, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000467 DBNGWZ010000000
VERSION     DBNGWZ010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2579704"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1244)
                     /locus_tag="AC16CX_14350"
     CDS             complement(267..1244)
                     /locus_tag="AC16CX_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013724.1"
                     /translation="MKLRYWLLGLALAGVSAVCGAQTAWSPQRNVEIVVGSAPGGSND
                     KTARQVERILVEKKLVSATLTVVNKPGGGSNIAFTYVNQHPADAHYLLIGTTALLSNH
                     IVGLSKVSYQDFTPIASLFNDYVVFAVNAESPIRTGKDLIERLKKNPNSVATGFATTL
                     GSHNHVAAGLLVKAVGGNARDLKAVAFKGSAEAITALLGGHIDLVTTAAGNAAPHVAA
                     GRMRVVGVAAPKRFGGALAAVPTWKEQGVDLVFGGWRAIMGPKGLSPAQVAYWEDALG
                     KATRVPEWKTDLEKNFWSDDFVTSQQFRKDLEKDYADMKTVLVDLGLAK"
     gene            complement(1325..2293)
                     /locus_tag="AC16CX_14355"
     CDS             complement(1325..2293)
                     /locus_tag="AC16CX_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013725.1"
                     /translation="MTVRAMTPALAMALFCALPSSYAQNYPVRPVRVIVPYAAGGNTD
                     FTARAIGAKLSDVFSQQVIVDNRPGGATNIGTEVVAKAPADGYMLLMGGASNAINMSL
                     FAKPPYNTLRDFAPVILCVKGANVLTTHPSVPARNLKELMALARAQPGKLNFASSGIG
                     SSNQMAGELFKVMAKINIVHVPYKGNAPAITDTIAGHVEMMFSGVPAIVPHLQSGRLR
                     AIGIGSLKRFPAIPEVPTFDESGLKGYEATTWFGLMAPIKTPKEIVGRWNVEVGKILA
                     SADIKERFINDGLEPMGGSPEAFGKFIHSEIDKYAGVIKAAGIPQQ"
     gene            complement(2330..3343)
                     /locus_tag="AC16CX_14360"
     CDS             complement(2330..3343)
                     /locus_tag="AC16CX_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3013726.1"
                     /translation="MTGNPIVTTLLVSHRLNAGYGARVAAAADREKITAEFLVLPPDP
                     AARLPDAECERVEVAFFSGDVFPDYSRQFFSAVRKAPRLKWLHVFNVGVDHPIYAEML
                     ARGVRLTTSAGSTAEPIAQTAIMGLLALARGLPHWLAAQRRRQWEPMRHGALPRDLRG
                     QIAIIVGLGKIGAEIARLARALGLHVIGVRRSPRHGGDPVDELHPPAALPDLAARCDW
                     LVIACPLTPETRGLVDAELIARLPKGARLINIARGEIVNEQALTAALRSGHLAGAYLD
                     VFEAEPLPPESPLWDLPNVLVTPHNSSAAAGNDERVLEIFLDNLGRWHRGEPLVNEVF
                     TGS"
     gene            3473..4606
                     /gene="dapE"
                     /locus_tag="AC16CX_14365"
     CDS             3473..4606
                     /gene="dapE"
                     /locus_tag="AC16CX_14365"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR3013727.1"
                     /translation="MAADTLELARQLIARRSVTPQDAGCLDLISSRLKPLGFHCERIS
                     ANGVENLWARRGEAAPLICFAGHTDVVPTGPLEHWASDPFIPTIREGALYGRGAADMK
                     TSLAAFVMAIEAFVAAHPRHAGSIAVLFTSDEEGIAVDGTVKVVEALRDRGEKMDYCV
                     VGEPTCVERLGDMIKNGRRGSLSGNLVVKGVQGHVAYPHLARNPVHEFGPVLAELAAT
                     EWDRGNEFFPPTTWQVSNISAGTGANNVIPGELHVKFNFRFSTASTLESLQTRVVGIL
                     DRHGLDYDLEWRYDGRPFLTKPGNLVDAVGRAIRTVTGIDTRLSTSGGTSDGRFIADI
                     CPQVVEFGPTNATVHKVNECIRVDEIEPLSRIYHQILVNLLVK"
     gene            4812..5054
                     /locus_tag="AC16CX_14370"
     CDS             4812..5054
                     /locus_tag="AC16CX_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013728.1"
                     /translation="MKQKVGTVLEQDVLRRAKRRAADENRPLSDVIQDALERYLSEGM
                     PEPARRDAAYQLFCERPMRLAPGQLKAVLEHDSWDL"
     gene            5051..5557
                     /locus_tag="AC16CX_14375"
     CDS             5051..5557
                     /locus_tag="AC16CX_14375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR3013729.1"
                     /translation="MNLDDIPAGSLCVVDTNVLIYAEQGVSPQAQRFVRRCSGGELKA
                     VLPQTVWQELAHKLMLAEAMMRGLTSGGNPAARLAAKPESIRGLTLHRAKVKSLLDLG
                     FGFEACQLTDLTERAFALQEKHGLLTNDAVVLAVAQRLKADALVSSDKAFRGITEPAV
                     YYPTDLRL"
     gene            5602..>5886
                     /locus_tag="AC16CX_14380"
     CDS             5602..>5886
                     /locus_tag="AC16CX_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3013730.1"
                     /translation="MRSDNIHELPPDLPVPEDDGACDHLAGMRLPQLALRSTAGRRVD
                     LSALEGRTVVYCYPRTGRPDREVPTGWNEIPGARGCTPQSCAFRDHFEELK"
BASE COUNT         1031 a         1883 c         1898 g         1074 t
ORIGIN      
        1 tcgtgtcttc ccccagctcg gagaggtgct tcgccagcag cttccgcacc tcgaggttga
       61 gcgcgttgcg tgcctcgggg cggttgatgc gcagcagcac cacgctttct gccgggcgtt
      121 cgaggaggac gagatcagtc atgatggcga gggatgaggg atgagggatg agggatgagg
      181 gatgaggagc cgcgcgcgtt gaacggcgcg ttccatgtcc gttcgaacgc gcgcagttaa
      241 accggagctt tgcttccggt ttaacatcat ttggcgagcc caaggtccac cagcaccgtc
      301 ttcatgtcgg cgtagtcctt ctccaggtct ttcctgaact gctggctggt gacgaaatcg
      361 tcggaccaga aattcttctc gagatcggtc ttccactccg gaaccctggt cgccttgccg
      421 agcgcatcct cccagtacgc gacctgggcc ggggacagcc ccttgggtcc catgatagcg
      481 cgccagccgc cgaaaacgag gtccaccccc tgctccttcc aggtggggac ggccgcgagc
      541 gcgccgccga agcgcttggg cgcggcgaca ccgaccaccc gcatgcggcc cgcggcgacg
      601 tggggagcgg cgttgccggc ggcggtggtg acgaggtcga tgtggccgcc gagcagggcc
      661 gtgatcgctt ccgccgagcc cttgaacgcg accgccttga ggtcgcgcgc attgccgccg
      721 accgccttga ccagcagtcc cgcggcgaca tggttgtggc tgccgagcgt ggtcgcgaag
      781 ccggtcgcga ccgagttcgg gttctttttc aagcgctcga tcagatcctt cccggtcctg
      841 atcggcgact cggcgttcac cgcgaacacg acgtagtcat tgaacaacga tgcgatcggg
      901 gtgaagtcct ggtagctcac cttcgacagc ccgacgatgt ggttggacag cagcgccgtc
      961 gtgccgatca acaggtaatg cgcgtcggcc ggatgctggt tgacgtaagt gaaggcgatg
     1021 ttgctgcccc cgccgggttt gttgacgacg gtgagcgtcg cgctgaccag cttcttctcg
     1081 accaggatgc gttccacctg gcgcgcggtc ttgtcattgc tgccgccggg ggcggagccg
     1141 accacgatct cgacgttccg ttgcgggctc caggcggttt gtgcgccgca cacggcgctc
     1201 acgccagcga gcgcaagccc gagcagccag tagcggagtt tcatgcttct cctcgtcagt
     1261 tgttgaagtc gggaaatgac attgtaagcg cggcgcggtg atgcgaccaa ggagggtcac
     1321 cgcattactg ctgcgggatg ccggccgcct tgatcacccc ggcgtacttg tcgatctcgg
     1381 agtggatgaa cttgccgaac gcctccggcg aaccccccat cggctccagg ccgtcgttga
     1441 tgaagcgttc cttgatgtcc gcgctggcga ggatcttgcc gacctcgacg ttccaccgtc
     1501 ccacgatctc cttcggtgtc ttgatcggcg ccatcaggcc gaaccaggtg gtggcttcgt
     1561 agcctttcag gccggattcg tcgaaggtcg gaacctcggg tatcgccgga aaacgcttca
     1621 agctgccgat gccgatcgcg cgcaaccggc cggactggag gtgcggcacg atcgccggca
     1681 cgccgctgaa catcatctcg acgtggccgg ctatcgtgtc ggtgatcgcc ggcgcgttac
     1741 ccttgtacgg cacgtgcacg atgttgatct ttgccatcac cttgaacagc tcgcccgcca
     1801 tctggttgga gctgccgatg cccgacgagg caaaattgag cttgccgggc tgggcgcggg
     1861 cgagcgccat cagctccttc aggttcctcg ccggcaccga aggatgcgtc gtcaacacgt
     1921 tggcgccctt cacgcacagg atgacgggcg cgaaatcgcg cagcgtgttg tagggcggct
     1981 tcgcgaacag gctcatgttg atggcgttgg atgcgccgcc catgagcagc atgtagccgt
     2041 cagcgggcgc cttcgccacg acctccgtgc cgatgttggt ggcgccgccg ggccggttgt
     2101 ccacgatcac ctgctggctg aagacgtcgg agagcttggc cccgatcgcg cgcgcggtga
     2161 agtcggtatt gccgccggcg gcgtacggca cgatcacgcg caccggcctc acggggtagt
     2221 tctgcgcgta agaggaggga agagcacaga atagcgccat cgcaagtgcg ggagtcattg
     2281 cgcggacagt catggttgtt ccctcctctt gaaataaccc acattcaaat catgacccgg
     2341 taaacacttc gttgaccagc ggctcgccgc ggtgccagcg gccgaggttg tcgagaaaga
     2401 tctccagcac ccgttcgtcg ttgcccgcgg ccgccgagga attgtgcggc gtgacgagca
     2461 cgttgggcag gtcccacagc ggcgactccg gcggcagcgg ctcggcctcg aagacatcga
     2521 gataggcgcc tgccaggtgc ccgctcctca atgccgccgt cagggcctgc tcgttaacga
     2581 tctcgccgcg cgcgatgttg atgaggcggg cgcccttggg cagcctggcg atcagctccg
     2641 cgtcgaccag cccccgcgtc tcgggcgtga gcggacaggc tatcacaagc caatcacagc
     2701 gcgcagccaa atccgggagc gctgccggcg ggtgcagctc gtccaccggg tcgccgccat
     2761 ggcgggggct gcggcgcacg ccgatgacat gcaggccgag tgcgcgcgca agccgagcga
     2821 tctccgcgcc aatcttgccg agccccacga ttatggcgat ctggcctctc agatcgcgcg
     2881 gcaacgcccc atgccgcatc ggctcccact ggcgccggcg ctgggctgcg agccaatgcg
     2941 gtaaaccgcg cgcaagcgcg agcagtccca tgatcgccgt ctgcgcgatc ggctcggcgg
     3001 tggagccggc agaggtggtg agccggacgc cgcgcgcgag catctcggcg tagatcgggt
     3061 ggtccacccc gacgttgaac acgtgcagcc atttgagccg cggcgctttc ctcaccgcgg
     3121 agaagaactg gcgtgaataa tcgggaaaca cgtcgccgga gaagaacgca acctcgacgc
     3181 gctcgcactc ggcatcgggc agacgtgctg cgggatcggg cggcagcacg aggaattccg
     3241 cggtgatctt ttcccggtcg gccgcggcgg cgaccctggc gccgtaacct gcgttcaatc
     3301 ggtgcgatac gagcagggtg gtcacaatgg gatttccggt cattcaaaaa atcggtgcgg
     3361 ttgggcccca ccactgcgcc gattttttgc gggcatttta cccgctctgc taagatgcga
     3421 gtctgctttg ctcatcaccc atcacccatc actcatcacg ctttccaggg aaatggccgc
     3481 tgatacccta gagctcgccc gccagcttat cgcgcggcgc tcggtgaccc cgcaggacgc
     3541 cggctgcctc gatctcatca gctcacggct caagccgctc ggcttccatt gcgagcggat
     3601 cagcgcgaac ggagtggaaa acctgtgggc gcgccgcggc gaagcagcgc ctctgatctg
     3661 cttcgccggc cacaccgacg tcgtgccgac cgggccgctc gagcactggg caagcgatcc
     3721 ctttattccc acgatccgcg agggagcgct ctacgggcgc ggcgccgccg acatgaagac
     3781 ctcgctcgcc gcgttcgtca tggcgatcga ggcgttcgtc gcagcgcatc ctcgccacgc
     3841 gggttcgatc gccgtgctgt tcacttccga cgaggaggga attgcggtgg acggcacggt
     3901 caaggtggtg gaagcgttga gggaccgcgg cgagaagatg gattactgcg tcgtcggcga
     3961 gccgacctgc gtcgaacgcc tcggcgacat gatcaagaac ggccgccgcg gctcgctctc
     4021 cggcaacctg gtggtgaaag gcgtgcaggg ccacgtcgcc tatccgcacc tcgcgagaaa
     4081 cccggttcac gagttcggcc ccgtgctcgc cgagctcgcg gccaccgaat gggaccgggg
     4141 caacgagttc ttcccgccca ccacctggca ggtctcgaac atcagtgccg gaaccggcgc
     4201 caacaacgtg atccccggcg aattacacgt gaagttcaac ttccgcttct ccaccgccag
     4261 cacgctggaa tcgctgcaga cgcgcgtcgt cggcatcctc gaccggcacg ggctcgatta
     4321 cgatctcgag tggcgctacg acggccggcc tttcctgacg aagccgggga acctggtgga
     4381 cgccgtcggc cgcgcgatca ggaccgtcac cggcatcgac acccggctct ccaccagcgg
     4441 cggcacctcc gacgggcgct tcatcgccga catctgcccg caggtggtgg agttcgggcc
     4501 gaccaacgcc accgtccaca aggtcaacga gtgcatccgc gtcgacgaga tcgagccttt
     4561 gagcaggatt taccaccaga ttctcgtgaa tctgctcgta aaataaggaa tttgctagtc
     4621 gactgccgat atgactggct gtagcgacct gattacaaac actatgtcat ggtattgcct
     4681 gctcgtatgc actgctgatc aggtagagtt cttgacgcgt ctagatttct agactattct
     4741 tgaaatcatg catggaagct tagaccacct ggcttgtgct cgggcggatc agaccgccgt
     4801 aggaggcaga aatgaaacag aaagtcggga ctgttttgga acaagacgtt ctgcgccgcg
     4861 cgaagcgacg cgccgccgat gaaaaccgtc cattgagcga cgtgatacag gacgcgctgg
     4921 agcgctatct ctccgaaggt atgcccgagc cggcgcggcg cgacgcagcc tatcaactct
     4981 tttgcgagcg gccgatgcgg ctcgcgcccg ggcagttgaa ggccgtgctc gaacacgaca
     5041 gttgggacct gtgaacctcg acgacatacc tgccggtagc ctgtgcgtgg tggacacgaa
     5101 cgtgctcatc tacgccgagc agggcgtttc gcctcaggcg cagcgcttcg tgcgccgttg
     5161 ttcgggcggc gaactcaagg cggtattgcc gcagaccgta tggcaggaac ttgcgcacaa
     5221 gctgatgctg gcggaagcga tgatgcgcgg ccttacctcg ggcgggaatc ccgcagcgcg
     5281 ccttgctgcg aaaccggagt cgatccgtgg actcacactc caccgtgcca aggtgaaatc
     5341 acttcttgat ttgggtttcg gatttgaagc ttgccagctc accgacctga cggagcgtgc
     5401 attcgccttg caggagaaac atgggcttct taccaacgac gccgtggtcc tggcagtcgc
     5461 acaacgtctg aaagccgacg ccctggtctc aagcgacaag gcgtttcgtg gtattaccga
     5521 gcccgctgtt tactacccga cggatctccg cctctgatat gagcgcgctc gagaaagcga
     5581 attcgtacgc ttgttcctca attgcgcagc gataacatcc acgagttgcc gccggacctg
     5641 ccggtgccgg aggacgacgg cgcgtgcgac catctcgccg gcatgcggct gccgcagctt
     5701 gcgcttcgct ccactgctgg gcgccgcgtg gatttgtcgg cgctcgaggg aagaaccgtc
     5761 gtgtactgct acccgcgcac cggccggccc gaccgggagg tgccgacggg atggaacgag
     5821 atacccgggg cgcgcggctg tacgccgcaa tcgtgcgcgt tccgcgatca cttcgaggaa
     5881 ctcaag
//
LOCUS       DBNGWZ010000468         4722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_62828, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000468 DBNGWZ010000000
VERSION     DBNGWZ010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_62828"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2111
                     /locus_tag="AC16CX_14385"
     CDS             36..2111
                     /locus_tag="AC16CX_14385"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3013731.1"
                     /translation="MSGGVIFALACAAIAILYGVVSINWILAQYEGNERMREIAAAVR
                     EGAIAYLKRQYTTIAVVGAVLFLVIGFTPALGWATAAGFLIGAVLSGLSGVIGMNVSV
                     RANVRTAEAARSGLNEALSLAFRGGAITGMLVVGLGLLGVTAFFWILHESAAHGAPIK
                     TILEPLVGLAFGGSLISIFARLGGGIFTKVADVGADLVGKVEAGIPEDDPRNPAVIAD
                     NVGDNVGDCAGMAADLFETYAVTIIATMLLGALLLSKPAVAPLAVAYPLALGGVSIIA
                     SIIGCFFVKATPGGKIMNALYKGVVWSGAIAAIGFIIVTYLMMGGISEQYPELTWWKL
                     LLSALVGLVLTALMVVITEYYTATEFSPVRHIAAASTTGHATNIIAGLALSMRATAWP
                     VIAVCASIYAAYTIAGLYGLAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMSHLPR
                     EVRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAAGKAVDFNLADPA
                     VLIGLFIGGLVPYLFGAMALEAVGRAAGSVVVEVRRQFKEVKGIMEGKAKPDYSRAVD
                     LLTRAAIKEMMVPSLLPVLVPIVVALGMNWLMGPGAGVKALGGVLMGTIVTGLFVAIS
                     MTTGGGAWDNAKKYIEDGNHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINI
                     VALLIVPLL"
     gene            2213..2737
                     /locus_tag="AC16CX_14390"
     CDS             2213..2737
                     /locus_tag="AC16CX_14390"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3013732.1"
                     /translation="MKYSFEDRKVICKGDYWIAPDAIVIGSVVLEANASVWFGCIVRG
                     DDDTITIGENSQLQDGCVLHVDRGFPITIGKNVSVGHMAMLHGCTIGDGTLIGIKAVI
                     LNGAKVGRNCLIGANALIAEGKEIPDGSLVIGSPGKVKRQLSEEEIRMINSAADHYVQ
                     EFRRYRETFKPDGA"
     gene            2775..2999
                     /locus_tag="AC16CX_14395"
     CDS             2775..2999
                     /locus_tag="AC16CX_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013733.1"
                     /translation="MNKYGFRIRTRGGSVVENLQVQARDRAEAEHKINQIYHHCEILE
                     CRELTPTLKKEGLDLEDVVSLINKQESGER"
     gene            complement(3123..3887)
                     /locus_tag="AC16CX_14400"
     CDS             complement(3123..3887)
                     /locus_tag="AC16CX_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3013734.1"
                     /translation="MAKIAVFNQKGGVGKTTTTLNLAALLARRGADPLVIDLDPQAHL
                     TGICNATVAASERSLYGHFSVGAALSGLIKFTGGGLKIIPSHLDLSKVDTQFGKGPHV
                     LNRLNAGIIRENLNTDRPILMDSCPMLGVLSLNGIFAADRVLVPISTDFLAIKGALQM
                     ERTLKALEQVLKKRVERRYVVTRFDARRKMSWDIDRELRERFGTEVCETRIAENVSVA
                     ESPSCDRDVFEHAPDSRGARDYTALLDELESSGFLR"
     gene            4121..4639
                     /locus_tag="AC16CX_14405"
     CDS             4121..4639
                     /locus_tag="AC16CX_14405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3013735.1"
                     /translation="METIRPEQADDIAAVQRVYHSAFPTAAEARLVDRLRANGKAVVA
                     LVAEDEGRVVGHILFSPVTLEPSLPVTAFGLAPMAVLPDHEKHGVGRRLVQNGLAECH
                     ARGAGFVVVLGDPAYYGRFGFEPAERHGLHNEYGAGDAFMVFELVAGALPRPGTLVKY
                     ASEFSALPKIQG"
BASE COUNT          829 a         1502 c         1549 g          842 t
ORIGIN      
        1 agttagaatt ctccccctca ccaaggggag atcgcatgtc cggtggcgtc atcttcgcgc
       61 tcgcgtgcgc cgcgatcgca atcctgtacg gcgttgtctc gatcaactgg atcctcgcgc
      121 agtacgaggg caacgagcgc atgcgcgaga tcgcggccgc ggtgcgcgaa ggcgcgatcg
      181 cgtacctgaa gcgccagtac accaccatcg ccgtcgtcgg cgcggtgctg ttcctcgtga
      241 tcggcttcac cccggcgctc ggctgggcga ccgccgccgg cttcctgatc ggcgcggtgc
      301 tctcgggcct gtcgggcgtc atcggcatga acgtctcggt gcgcgccaac gtgcgcaccg
      361 cggaggccgc gcgcagcggc ctgaacgagg cgctctccct cgccttccgc ggcggcgcca
      421 tcaccggcat gctggtggtg ggcctggggc tgctcggcgt caccgccttc ttctggatcc
      481 tgcacgagtc ggccgcgcac ggggccccga tcaagacaat cctcgagccg ctggtgggac
      541 tcgctttcgg cggctcgctg atctcgatct tcgcgcgtct cggcggcggc atcttcacca
      601 aggtggcgga cgtcggcgcc gacctcgtgg gcaaggtcga agccggcatc cccgaggacg
      661 acccgcgcaa ccccgcggtg atcgccgaca acgtgggcga caacgtcggc gactgcgccg
      721 gcatggcggc ggatctgttc gagacctacg ccgtgaccat catcgccacc atgctgctcg
      781 gcgcgctgtt gttgtcgaag ccggcggtcg ccccccttgc cgtggcctat cctctcgcgc
      841 tgggcggcgt ctcgatcatc gcttccatca tcggctgctt cttcgtcaag gccacgcccg
      901 ggggcaagat catgaacgcg ctctacaagg gcgtggtctg gtccggtgcc atcgcggcga
      961 tcggcttcat catcgtcacc tacctcatga tgggcgggat cagcgagcag tatccggaac
     1021 tcacgtggtg gaagctgctg ctctcggcgc tggtcggact ggtcctgacg gccctgatgg
     1081 tagtcatcac ggagtactac accgcgaccg agttctctcc cgtgcgccac atcgccgcgg
     1141 cctccaccac gggccacgcg accaacatca tcgccgggct cgccctctcc atgagggcga
     1201 ccgcctggcc ggtgatcgcg gtgtgcgcga gcatctacgc cgcctacaca attgccggtc
     1261 tctacgggct ggcaatcgcc gcaaccgcga tgctctcgat gaccggcatg atcgtcgcgc
     1321 tcgacgccta cggcccgatc accgacaacg cgggcggcat cgcggagatg tcgcacctgc
     1381 caagggaagt gcgcgccatc acggatccgc tcgacgcggt aggcaacacg accaaggcgg
     1441 tgaccaaggg ttatgccatc ggctccgcgg ggctcgcggc gctggtgctg ttcgccgatt
     1501 acacgcacgc gctggaggcg gcgggcaagg cggtggattt caacctcgcc gatccggccg
     1561 tgctcatcgg actgttcatc ggcggcctcg tgccctacct tttcggcgcg atggcgctgg
     1621 aggccgtggg ccgcgcggcg ggctcggtcg tggtcgaggt gcgccgccag ttcaaggaag
     1681 tcaagggcat catggaaggg aaagcgaagc ctgattactc gcgcgcggtg gacctgctga
     1741 cgcgcgccgc gatcaaggag atgatggtgc cgtcgctgct gccggtgctg gtgccgatcg
     1801 tggtggcgct ggggatgaac tggctgatgg gaccgggcgc gggcgtgaag gcgctgggcg
     1861 gagtgctgat gggcacgatc gtcaccggtc tcttcgtcgc gatctcgatg acgaccggcg
     1921 gcggcgcctg ggacaacgcc aagaagtaca tcgaggacgg caatcacggc ggcaagggct
     1981 ccgacgcgca caaggcggcg gtgaccggcg acacggtcgg cgatccctac aaggacaccg
     2041 ccgggccggc ggtcaacccg ctcatcaaga tcatcaatat cgtcgcgttg ctcatcgtgc
     2101 cactgctgta gcgcggcatg atcgctagca ttcagcgctc agttttcagc cgtcagcgaa
     2161 atccattgaa cgatttacgc tgaccgctga tagctgtacg ctgacagccg atatgaaata
     2221 ctcctttgag gaccgcaagg tcatctgcaa gggcgactac tggatcgcgc cggatgcgat
     2281 cgtgatcggc tcggtggtgc tggaggcgaa tgccagcgtg tggttcgggt gcatcgtgcg
     2341 cggcgacgac gacaccatca ccatcggcga gaattcgcag ctgcaggacg gctgcgtgct
     2401 gcacgtggat cgcggctttc cgatcacgat cgggaagaac gtcagcgtcg gccacatggc
     2461 gatgctgcac ggctgcacca tcggcgacgg gacgctgatc ggcatcaagg cggtgatctt
     2521 gaacggcgca aaggtcggca ggaactgcct gatcggggcc aatgcattga tcgccgaggg
     2581 caaggagatc cccgacggct cgctcgtcat cggctcgccg ggcaaggtca agcggcagct
     2641 ctcggaagag gagatccgca tgatcaactc cgccgccgat cattacgtgc aggaattcag
     2701 gcgctaccgg gaaactttca agcccgatgg ggcttgagtg actcacgact cctgactatg
     2761 tcccagaacc cgccatgaac aagtacggat tcagaatcag gacacgtggc ggctccgtcg
     2821 tggaaaacct ccaggtgcag gcgcgcgatc gcgccgaggc cgagcacaag atcaaccaga
     2881 tctaccacca ctgcgagatc ctggagtgcc gggagctcac gccgaccttg aagaaggaag
     2941 gactcgatct cgaggacgtg gtttcgctga tcaacaagca ggaaagcggt gagcggtgag
     3001 cggtgagcgg tgagcggtga gcggtgagcg gtgagcggtg agcggtaaat tggacgcccc
     3061 gcgtggccgg taattcaccg tctgcggctt accgcttacg gcttaccgct tacctttacg
     3121 atttaccgaa ggaatccgct ggattccagt tcgtcgagca gcgccgtata gtcccgggcg
     3181 ccgcggctgt cgggcgcgtg ctcgaacacg tcgcgatcgc aggaagggct ttccgcgacg
     3241 ctcacgttct ccgcgatccg cgtctcgcac acttccgtac cgaagcgctc gcgcaactcc
     3301 cggtcgatgt cccacgacat cttgcggcgc gcgtcaaagc gcgtcacgac gtagcgccgc
     3361 tcgacgcgct tcttcaacac ctgctccagc gccttcagcg tgcgctccat ttgcagcgcc
     3421 cccttgatcg cgaggaagtc cgtcgagatc ggcaccagca cgcggtcggc ggcgaagatg
     3481 ccgttcaaag agagcacgcc cagcatgggg cacgagtcca tcaggatcgg ccggtcggta
     3541 ttgaggttct cccggatgat gccggcgttg aggcggttga ggacatgcgg gcccttgccg
     3601 aactgcgtgt ccaccttcga caggtcgaga tgcgaaggga tgatcttcag accgccaccg
     3661 gtgaacttga tgaggccgga aagcgcggcg cccaccgaaa aatggccgta caggctgcgc
     3721 tcggacgccg ccaccgtggc gttgcagatc cccgtgagat gggcctgtgg gtcgagatcg
     3781 atcaccagcg gatcggcgcc acgccttgcc agcagcgcag caaggttgag cgtcgtggtc
     3841 gtcttgccca caccgccttt ttggttgaat accgcgatct tcgccactgc ttccctcccc
     3901 agaaggggat cgttagatta gtcctcgaat caggaggctg gcaagaactt ttgtcacgcc
     3961 tatttaagaa acggccggaa ggccctaggg aaaaaccatt aaagtcaaaa ggaattgcca
     4021 ccagacgagc tgccgcgccg accgggcaaa acaaaatatg gttcggacgg ggtggccgct
     4081 gcgtttggct gtgataatcg agctttgccg ctccgacccc gtggagacca tccgacccga
     4141 gcaagccgat gacatcgccg cggtgcagcg tgtctatcat tcggccttcc ccaccgctgc
     4201 cgaggcgcgc ctggtggacc ggttgcgcgc gaacggaaaa gcggtggtgg cgctcgttgc
     4261 ggaagacgaa ggccgggtgg tcggccatat ccttttcagc cccgtgacgc tcgaaccgtc
     4321 gctccctgtc acggcattcg ggctggcccc gatggcggtg ctgccggacc acgagaagca
     4381 cggcgtcggc cggcggctgg tccagaacgg tctcgccgag tgccacgcgc gcggggccgg
     4441 ctttgtcgtc gtgctcggcg atccggctta ctacggccgg ttcggcttcg aaccagcgga
     4501 gcggcacggt ttgcacaacg aatacggcgc cggcgatgcg ttcatggtgt tcgagctggt
     4561 tgccggcgcg cttccccggc cgggaacgct ggtcaaatac gcctccgaat tttcggccct
     4621 gccgaaaatt caaggatgag ggatgaagga tgacccccgc cgtacgggag ggaaaatctc
     4681 agccttctat cttcatcctt ccgccttcat ccttcaggcg tg
//
LOCUS       DBNGWZ010000469        14521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2454904, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000469 DBNGWZ010000000
VERSION     DBNGWZ010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2454904"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="recR"
                     /locus_tag="AC16CX_14410"
     CDS             complement(<1..287)
                     /gene="recR"
                     /locus_tag="AC16CX_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080181.1"
                     /note="involved in a recombinational process of DNA
                     repair, independent of the recBC complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3013736.1"
                     /translation="MKTPSSLEELISALRCLPGVGPRSAQRMAYHLLQRDPSGAARLA
                     AALTQTLSRIRHCERCNNYAEEAVCALCSSSRRDPALLCVVEGPGDLLMME"
     gene            complement(380..703)
                     /locus_tag="AC16CX_14415"
     CDS             complement(380..703)
                     /locus_tag="AC16CX_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR3013737.1"
                     /translation="MKGGLGNLMKQAQLVQENMKKLQEQIAIMEIEGQAGSGLVKVVM
                     TGRHEVKRVTIDPGLLSEDKDMLEDLIAAAFNDAARRVEAVTQEKMAGMTAGLPLPPG
                     MKLPF"
     gene            complement(818..2377)
                     /gene="dnaX"
                     /locus_tag="AC16CX_14420"
     CDS             complement(818..2377)
                     /gene="dnaX"
                     /locus_tag="AC16CX_14420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR3013738.1"
                     /translation="MTQVLARKWRPRTFSELVGQEHVVRALENALAQQRLHHAYLFTG
                     TRGVGKTTLARIIAKALNCEPGVTAAPCGRCSACLEIDAGRFIDLIELDAASNTQVDN
                     MRELLENALYAPTAGRYKVYIIDEAHMLSRNAFNAMLKTLEEPPQHVKFILATTDPQK
                     IPVTVLSRCLQFNLKQIPQQQIRNRLQHVLEAEDIRYEPPALALIARAAQGSLRDALS
                     LLDQAIAHGGGSVGQESVRAMLGVVGNDHLHAILRALAARDGPALADQAARMEALSLS
                     FDSALQDLASLLHRLSLAQTVPDALAEDDPDRAVIEELARLFSPEDLQLYYQIAIQGR
                     GDLGLAPDERTGFTMTLLRMLAFVPVEEGTGIPASPRAVAPRTEHASPPVPAAASKKN
                     SGDAAWQELVEELGLSGMARMLAQHCELVARDATRIELKIAQAHQHLLDGPYQERLKS
                     ALEQRFGGRLRLVIRPADSAGASPAAIADREKQQRQAQAIAAIEQDPLVRELVENFDA
                     RIVESSIKPVQ"
     gene            complement(2457..2963)
                     /locus_tag="AC16CX_14425"
     CDS             complement(2457..2963)
                     /locus_tag="AC16CX_14425"
                     /EC_number="3.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF020965.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HIR3013739.1"
                     /translation="MAAVLLLVAVPAVAVDAIAVEAGSGDSTDMGRVAIQWDWGKRWF
                     QGADWHVGGYWDVALGYWHRNALAGQNGDITEIGLTPVFRLQRNGLVGPYLEAGIGAH
                     LLSDTTIGDKRLSTRFQFGDHLGLGYRFGAKRTLDLGYRFQHLSNAGIKQPNNGINFH
                     QARLQYHF"
     gene            complement(3048..3146)
                     /gene="ffs"
                     /locus_tag="AC16CX_14430"
     ncRNA           complement(3048..3146)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16CX_14430"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3304..5361
                     /gene="acs"
                     /locus_tag="AC16CX_14435"
     CDS             3304..5361
                     /gene="acs"
                     /locus_tag="AC16CX_14435"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3013740.1"
                     /translation="MPKKKLLRPSKEQQSADISEAQIAVHWREEGYFSPPAKFIAQAN
                     LTDKAVLKRFSLDKFPEYYKEFADLLDWYKRWKKTLDTSNPPFWRWFVGGEINACYNC
                     VDRHLARHKNKTAIHFVPEPEHEAVQHITYQELFVRVNEFAVLLRDFCGLKAGDRVTL
                     HMPMVTELPIAMLACARLGVIHSQVFSGFSGKACADRVVDSESRVLITMDAYYRAGAL
                     LDHKEKADIAVTEAAKERHKVDKVLIWQRYPGKYSSPTKLVEGRDVIVNDLMRKYRGK
                     RVEPVRLRAEAPLFLMYTSGTTGRPKGCQHSIGGYLAYVTWTSKYIQDIHPEDVYWCM
                     ADIGWITGHSYIVYGPLALAASSVVYEGVPTHPDAGRPWRIAEDLGVNIFHTSPTAIR
                     ALRRAGPDEPLKYHHHFKHMTTVGEPIEPEVWKWYYKVVGKSEAVIVDTWWQTENGGF
                     LCSTVPGIHKMKPGSAGPGIPGIHPVIYDDQDKELPAGSGKAGNICIRNPWPGGFQTI
                     WKDPDRFVRQYYTRYCKNPKSKDWRDWPYMAGDGAVQAADGYYRILGRIDDVINVAGH
                     RLGTKEIESAALIIPEVAEAAVVPVADPIKGRVPDLYVSLKPGFTASKEIADKVSGAV
                     VSEIGPIARPRAVVIVPDMPKTRSGKIMRRVLAAISNHKDPGDVSTLANPEVVDKIKE
                     LVK"
     gene            5504..5596
                     /locus_tag="AC16CX_14440"
     tRNA            5504..5596
                     /locus_tag="AC16CX_14440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5538..5540,aa:Ser,seq:gga)
     gene            complement(7151..8194)
                     /locus_tag="AC16CX_14445"
     CDS             complement(7151..8194)
                     /locus_tag="AC16CX_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3013741.1"
                     /translation="MARLRIIGKSYVLDWRDVEGTRHRDPLGRVGIFPKDETRRILRN
                     KQLALQPGHKLIQRRAPLFDDFAKEYVKWHALEFPTSHYRIEQILEQYLIPAFSGDRL
                     DEITPKRAEEYKRARGTAETPPRSQTLTKEIRTLKAILNKAVEWKDLEANPIARVVGP
                     KNLDSKPPRFFTAAELARIYAACRATVNAGEGPQPNPLHAHWWRLYANTGLRRAEGLM
                     LRWDWIGREAMKILSTDKERTKSGKWREIPLTAGAQEALEGLPRDGAHVLPRISLPSL
                     SRAATRDIARADVEGGSLHTFRHTYCSHLVMAGVPLRTVQQLAGHSTIAVTERYAHLT
                     PDHLAGAGRAISL"
     gene            complement(8693..8851)
                     /locus_tag="AC16CX_14450"
     CDS             complement(8693..8851)
                     /locus_tag="AC16CX_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013742.1"
                     /translation="MSDLILSLDELKPAVGSALLGTRSCPEGAHPTGSRVHRRKALQA
                     KAKSEAKI"
     gene            9382..10299
                     /locus_tag="AC16CX_14455"
     CDS             9382..10299
                     /locus_tag="AC16CX_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR3013743.1"
                     /translation="MSAEQRNLYDLIGMSRDASQESIVQRCLSLGEELGPDKNAGNLR
                     TALLFAQIEKAYEILGDPVRRAAYDAELREKEAGYASSVGPRIDRGPDYPANARRWGQ
                     FSRSKLIAIAVVVIAAIALTGGIAAISVRNKEAARAAEAQRVAEETARRAAEERAREL
                     EARAKAAEERVRMAAVRAAEESAPRVRRMTASELRADKEDLARVENVRAQCEAAGRSV
                     ERSRGGGTFSDSIGTFVGAQLAIPICMQSERAVKDRLRYEALRSEDTAAAMKELELQR
                     AARIERQRNRPVITNCTTDRYGNTNCISR"
     gene            10377..10832
                     /locus_tag="AC16CX_14460"
     CDS             10377..10832
                     /locus_tag="AC16CX_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013744.1"
                     /translation="MTMQFITTLAEYTRPWKLVTLAIGVALLIVGSFYYQAPDWDIPI
                     SLIMAILAYLTAPWSLRVILERRWRLWPAMLLATWFTVDGSYWLYWSFKDPVALELMR
                     EANFFASLALYGMCGVAWLYRGSLRQLILEIGALRRRAASAHPDRRPPR"
     gene            10848..12131
                     /locus_tag="AC16CX_14465"
     CDS             10848..12131
                     /locus_tag="AC16CX_14465"
                     /inference="COORDINATES: protein motif:HMM:NF016488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013745.1"
                     /translation="MRYTEAQAQEFDAAYQSLTPHLRVAEGRLLELVQRAIARIDDKR
                     LVRAQVRGTRIKALDSVRRKAEEHGVSPKEACLSIGDLVGARVVCNTTEDVRRFKELL
                     RRELPSGEPIEEDDHMANPRTSGYRGLHLKFRINIGEGLIPVLIPCEVQLRTLLQDSW
                     AELVHSDIYKEGATLPEDLRGRTQDLAGVLASADQIASRIRARVMQETKVEPESIRLD
                     TVTKEGLAYVFAEVFGRTPPDYVVQAAYEACLDAGLAAVSRLRGKLSEKSFIDAVTKA
                     YRDELRLGFDISTEDIFTLAPGAATKGDKAAAKKARRLARADRSEIETVWRNQVLGEL
                     PDNWERFLEELEAKHVDVEAVAEALGVASACTICGATIVDHYSFFEAAARHYGVDAEY
                     DHDVIQALSNATDCWADDDYPNLCSYHGYKASKDD"
     gene            complement(12264..13232)
                     /locus_tag="AC16CX_14470"
     CDS             complement(12264..13232)
                     /locus_tag="AC16CX_14470"
                     /inference="COORDINATES: protein motif:HMM:NF018684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR3013746.1"
                     /translation="MAKNRTVQDLLEIKAREKYGTRASIYYGRISALKSAFEAGSFKA
                     GDEVLRYFPIGAIAAVESYFRAAYAELLDAGEPYASRIEPLIRDLRIDWRAVRAIEGK
                     AVSSGEVVAHSLSLSSLGDIQRVMNGLLGVDFLDLLRRTPKSGMAEAQSAHDLVLPYP
                     DQCFKYVSRAFELRHIFCHESATSVTIDDLEVLACLNWCQSFLYATGELLLRALFTDW
                     GLSQAELTNKASIKLDSARQELARVENELRRVFVGYDRAKTLFREVQDKWHDFANANA
                     DLVVFLWEGGSGYPMAHSLALEEATKRRTEELTKQLREWAFVRDPA"
     gene            complement(13610..14338)
                     /locus_tag="AC16CX_14475"
     CDS             complement(13610..14338)
                     /locus_tag="AC16CX_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013747.1"
                     /translation="MSSNDEKPAKIVTSKDLIRLKQLIALLCIQREMKIPEACDYIIA
                     LLERATDFKIYDTGYEGLPEVLELNDGDRRSNALLTLFNSHYWDRAEPLPENVNDWSR
                     DDRKAEFLAIEQLDAATLLGLSPAQWDELISRYAARHWRPSHDAAKLIVSLTEQDCLN
                     RDHAHFSLKLAAAIEAWRAVSTHPESFRGKSVKQALIEWLQRNAEKFALVKNDGSPNE
                     QGIEEIAKIANWDTKGGAPKTPEK"
BASE COUNT         2819 a         4289 c         4457 g         2956 t
ORIGIN      
        1 tgctccatca tgaggaggtc tcccggccct tccaccacgc acagcagcgc gggatcgcgc
       61 cgggacgagc tgcacagcgc gcaaacggcc tcctcggcat agttgttgca cctttcgcaa
      121 tgccggatcc tggaaagcgt ctgggtcagg gctgcggcga ggcgcgccgc gccagaggga
      181 tcgcgttgca acaggtgata ggccatgcgt tgcgccgacc tcggccccac gccaggcagg
      241 cagcgcaggg cggaaatcag ctcttcgagc gatgaaggcg tcttcattcc agaagctgtg
      301 agcggtaagc agtcagcagt gagtcgttta atccgggaac aagaaaaccg ctcaccgcta
      361 accgttcact gcttacgggc taaaacggca gtttcattcc cggcggcagc ggtaagcccg
      421 ccgtcatgcc cgccatcttt tcctgcgtga ccgcctccac ccgccgcgcc gcgtcgttga
      481 atgccgcggc gataaggtct tcgagcatgt ccttgtcctc ggagagcaag ccggggtcga
      541 tggtcacgcg cttcacttcg tgacggccgg tcatgacgac cttcaccagc cccgagcccg
      601 cctggccttc gatctccatg atcgcgatct gctcctgcag cttcttcatg ttctcctgca
      661 cgagctgcgc ctgcttcatc agattgccaa gcccgccttt catttgctgc tccttcgggg
      721 tgagcggtta gcggtgagca gtgagcagtg agcggtagaa tcaaacttat ccgtttgccg
      781 ttgaccgctt accgcttact gtttaccgct taccgtttca ttgcaccggt ttgatcgagg
      841 actccacgat gcgcgcgtcg aagttctcca ccagctcgcg caccaacggg tcctgctcga
      901 tggcggcgat ggcttgcgcc tgccgctgct gcttttcccg gtccgcgatc gcagccggcg
      961 aagcgccagc gctgtcggcg ggccggatga ccagcctgag cctgccgccg aagcgctgtt
     1021 cgagcgccga tttcagccgc tcctggtacg ggccgtcgag caggtgctgg tgagcctggg
     1081 cgatcttcag ttcgatgcgc gtcgcatcgc gcgccaccag ctcgcaatgt tgcgccagca
     1141 tgcgcgccat gccgctcagg ccgagctcct ccacgagctc ctgccaagca gcgtcaccgg
     1201 agtttttttt tgatgccgcg gctggcacgg gcggggaagc gtgctcggtg cggggggcga
     1261 cggcgcgagg cgatgccggg atgcccgttc cctcctccac cggaacgaaa gccagcatcc
     1321 tgagcagcgt catcgtgaaa ccggtgcgct cgtcgggcgc cagcccgaga tcaccgcggc
     1381 cctggatcgc gatctggtag taaagctgca ggtcctccgg cgaaaacagc ctcgccagct
     1441 cctcgatcac ggcgcggtcg ggatcgtcct ccgcgagcgc gtccggcacc gtttgcgcca
     1501 gcgagagccg gtgcaacaac gatgcgagat cctgcagggc ggaatcgaac gaaaggctga
     1561 gcgcctccat gcgggcggcc tggtcggcca gggcgggccc gtcccgcgcc gcgagcgcgc
     1621 gcaggatggc gtggagatgg tcgttaccga ccacgccgag catggcgcgc acggattcct
     1681 gcccgacgct gccgccgcca tgggcaatcg cctggtcaag caggctcaac gcatcgcgca
     1741 ggctgccctg ggcggcgcga gcgatcagcg ccagcgccgg cggctcgtag cggatgtcct
     1801 cggcctccag tacgtgctgc agccggttcc ggatctgctg ctgcgggatc tgcttgaggt
     1861 tgaattgcag gcagcgcgac agcaccgtga ccgggatttt ctggggatcg gtcgtggcga
     1921 ggatgaactt gacgtgctgc ggcggctcct ccagcgtttt cagcatcgcg ttgaacgcgt
     1981 tgcgcgacag catgtgcgct tcgtcgatga tgtaaacctt gtagcgtcca gcggtgggag
     2041 catagagcgc gttttccagc agctcgcgca tgttgtccac ctgggtgttg gaggcggcat
     2101 cgagctcgat caaatcgatg aagcgccccg catcgatctc caggcatgcg gagcatcgtc
     2161 cgcagggggc ggcggtcacg ccgggctcac agttgagcgc cttggcgatg atgcgcgcca
     2221 gcgtcgtctt gcccaccccg cgcgtcccgg tgaaaagata ggcgtgatgc agccgctgct
     2281 gcgcgagcgc gttctcgagc gcccgcacca cgtgctcctg ccccaccagc tcggaaaaag
     2341 tccgggggcg ccacttgcgg gcgagaacct gggtcatacg gaagcgatca gcactcagag
     2401 attagcgatc agccgtattt tactgctgac tgccgattgc tgaccgctgg tggccgtcaa
     2461 aagtgatatt gcagccgggc ctggtggaaa ttgatgccgt tgttgggctg cttgatgccg
     2521 gcgttcgaca ggtgctgaaa gcggtaaccc aggtccaggg tgcgcttcgc gccgaaccgg
     2581 taaccgagcc cgaggtgatc gccgaactgg aaccgcgtgc tcaagcgctt gtcgccgatc
     2641 gtggtgtcgg acaggaggtg ggccccgatt ccggcctcga gatacggccc caccaggcca
     2701 ttacgctgaa ggcgaaatac cggcgtcagg ccgatttcgg tgatgtcccc gttctggccg
     2761 gcaagcgcat tgcgatgcca gtagccgagc gccacgtccc agtaaccgcc gacgtgccaa
     2821 tcggccccct ggaaccaacg cttgccccag tcccactgga tggcaacccg ccccatgtcg
     2881 gtgctgtcgc cgctgccggc ttcgactgcg atcgcgtcca cagcaaccgc cggcacggcc
     2941 accagcaaga gaacggcagc catcacccct gcagataatt tcttcttcat cattcttgtt
     3001 gttcgctcct gtgcgggacg cgcgggatga taacccatgg cgaaatggga ggcgagcctt
     3061 gcccccggca cttgcaaaaa atagctgtgg ctgcttcctt ccggacctga ccaggttcac
     3121 taccttgcaa tgcggggagg cccgccatgt gagttgtcgg catattatgc ccgcgcgccg
     3181 tattccgcgc cagaaacaag cgcatcttta caacaagccg ctttgaagta aaatcgggag
     3241 cccgaggcgc accggatgcg cctcccgagc gcgtttcgtg gaagatcaag gagaggaatt
     3301 gccatgccga agaaaaagct tctccgcccg tccaaggaac agcagagcgc cgatatttcc
     3361 gaagcgcaga tagcggtgca ttggcgcgag gagggatatt tctcgccgcc ggccaagttc
     3421 atcgcgcagg ccaatctcac agacaaggcc gtgttgaagc gcttcagcct cgacaagttt
     3481 ccggaatact acaaggaatt tgcggacctc ctcgactggt acaagcgctg gaagaaaact
     3541 ctcgatacca gcaatccgcc gttctggcgc tggttcgtcg gcggcgagat caacgcctgc
     3601 tacaactgcg ttgaccggca tctcgccaga cacaagaaca agacggcgat ccattttgtg
     3661 ccggagcccg agcatgaggc agtccagcac atcacctatc aggagctgtt tgtccgcgtc
     3721 aacgagttcg cggtcctgtt gcgcgatttc tgcgggctca aggcgggaga ccgggtgacg
     3781 ttgcacatgc cgatggtgac ggagctgccc atcgccatgc tcgcctgtgc gcggcttggt
     3841 gtcatccact cccaggtgtt cagcggcttc agtggcaaag cctgcgccga ccgcgtcgtc
     3901 gattccgaaa gccgggtgct gatcacgatg gacgcctatt accgtgcggg cgccctgctc
     3961 gaccacaagg agaaagcgga tatcgcggtc accgaggccg ccaaggaacg ccacaaggtc
     4021 gacaaggtgc tgatctggca gcgctacccg ggcaagtact cgagcccgac gaagctcgtg
     4081 gaagggcgcg acgtcatcgt caacgatctc atgaggaaat atcgcggcaa gcgcgtcgag
     4141 cccgtgcgcc tgcgggccga ggctccgctt ttcctcatgt acacaagcgg caccacggga
     4201 cggcccaagg gatgccagca ctccattggg ggttacctcg cctacgtcac ctggacgtcc
     4261 aaatacatcc aggacatcca tcccgaggac gtttactggt gcatggcgga catcggctgg
     4321 attacaggtc attcatatat cgtttacggt ccgcttgcgc tcgccgcctc ctcggtcgta
     4381 tacgagggcg tgccgaccca tccagacgcg ggacggccgt ggcgcatcgc cgaggacctc
     4441 ggcgtcaata tcttccacac ctcgcccacg gcgattcgcg cgctgcgccg ggccgggccg
     4501 gacgagccgc tgaagtacca ccaccacttc aagcacatga cgacggtagg cgagccgatc
     4561 gaacccgagg tctggaagtg gtattacaag gtagttggca agagcgaagc ggtgatcgtg
     4621 gacacctggt ggcagacaga aaacggcggc ttcctttgca gcacggtgcc ggggatccac
     4681 aagatgaaac ccggcagtgc gggtcccgga attcccggca ttcatccggt tatttatgac
     4741 gaccaggata aggaactgcc ggccgggtcg ggcaaggcgg gcaacatctg catccgtaac
     4801 ccctggcctg gcggattcca gacgatttgg aaggatcccg accgcttcgt gcggcagtac
     4861 tacacgcgct actgcaagaa tcccaagagc aaggactggc gcgactggcc ctacatggcg
     4921 ggcgacggcg cagtgcaggc ggcggacggc tattaccgca tcctcggccg catcgacgac
     4981 gtgatcaacg tggccggcca tcgtctcggc accaaggaaa tcgagtcggc agcgctcatc
     5041 atccccgaag tggcggaagc ggcggtcgtc ccggtcgccg acccgatcaa gggcagggtg
     5101 ccggatcttt acgtctccct taagccgggc ttcacggcat ccaaggagat cgcggacaag
     5161 gtgtcggggg cggtggtttc cgagatcggc ccgatcgcca ggccgcgtgc cgtggtcatc
     5221 gttcccgata tgcccaagac gcgctcgggc aagatcatgc gccgggtgct cgcggcgata
     5281 tccaaccaca aagatccggg cgacgtctcc acgctcgcca accccgaggt cgtggacaag
     5341 atcaaggagc tggtcaagta acgactgcac ccggacgagc cggcgcgttt ctcctgcgat
     5401 cgaacggatc gatcggtcgc tcgtccccgg gaaatcccac ccgcgttgaa aacgctgccg
     5461 ccggcccgac cgcgccgggc gatctgttaa agtacgcgcc ttcggagagg tgaccgagcg
     5521 gttgaaggtg ctcgcctgga aagcgagtat acggtcaaaa gccgtatcgc gggttcgaat
     5581 cccgccctct ccgccagaat aatatataac taattgatca taaagtatat tgttatctgc
     5641 ttagcacttt acccacgctt ctacccacaa atcagacccg cggctagcta ggggtcgctg
     5701 ttactgaatc gtactgcctg cgcccccgcc agtcgtcgcc gtgcccaagc aatgagcatg
     5761 catgttagcc gacccgcctt agggaatgaa tggaatgtat ggaaactttg atcctgtatt
     5821 ggtcgtgtgg gccggcgcag ctcgggtctg accgatactt ccccctgcca ccgacttccg
     5881 cagttacgtc cgctcgtgtc ccgtcggact tccgcaggaa ggaaccgctg agcgtgcggg
     5941 cggtctctgg atgctacgtg cggagatcag gccgcgtcgc cgctaaccgc gcgatgtttg
     6001 acaatgtcat acaaatcaag aagatagcta ttgacggtgc atttaaacgc gcgtagtgtt
     6061 gtgtctggct ctagaaaagc cgacgggacg caccaccgcg cagtctgttt tactgttcct
     6121 ggccgggggt gtggctgaat gcaagactcg gcgtaagccg ggaaataagc cgcacagcct
     6181 cctatcgctg tttctagacg cccggccgcc tcagtctgtt actgggcatt ggtctagaaa
     6241 accagatagg agcgcaccat gtttagcacg caacccgcac gcagcgcact taacgacgcg
     6301 cttaagctcg cagtccttca aaccgtcctg caagtcgagc gcgactcacg cacggtcgat
     6361 accgcacgta tccgcgcggt ggcagacgaa ctacacgccg tagtggatcg cgctgccgcc
     6421 tgagcagtcc ggcgacatca cgaccggggc ggcgtatcgc cccggttttt tctttcccgc
     6481 ttccccctag ctacccgcgc cgcccgttcc tgctggactg tcagcccgcc cgacgctggc
     6541 cggcgctgca acgagcgaat ttcaaatcga gctggcgagg ggtgcgtcct gggggtaagc
     6601 gcgtgtgacc gccgggcgcg ttggatctgt cccgggaaaa tttgggtcat tttctaagct
     6661 cgtaaagaaa tttactggcc ccgtaaggga gtcgctgcct caccgcctga cactccaccg
     6721 gctcacctcg aaacggcccg aaaaatttgc ccccgcgacg cagcctgtaa agatcttgac
     6781 tatgtaactg tatgtcgcaa cgagtaattc tcaaggacgt aaagaaagtg acatgcgcga
     6841 cgtcgcgttc tttacgtgcg gcgcggtgtc gcggtggggc gcggcagcag gtccatgggc
     6901 gaaggggtcc gtgtttcgga cggcgccggg actgcatgcg atgggtgcgc cgggggttgg
     6961 gatgaaaccg gagctcgcgc cggctaaatg cttacgctta gctggaaccc tcgtctggtg
     7021 aaccggtggc gcgcggcctc gagaccggtc ttcgcgggcc gctgccggat agtgggacgg
     7081 tagcgcctgc cgttgtactg gacgtcgaac tggatgcgcg ctttgccctt cggggaccgc
     7141 gcccgccatg ctacagactg atcgcccgcc ccgcgccggc caagtggtcc ggcgtgaggt
     7201 gcgcgtagcg ctcggtgacg gcgatcgtgg agtgcccggc gagctgctgc acggtgcgca
     7261 acggcactcc ggccatgacg aggtgcgagc agtacgtgtg gcggaacgtg tggaggctcc
     7321 cgccctcgac gtcggcgcgc gctatatcgc gcgtcgcggc ccgtgagagc gatggcaggc
     7381 tgatccgagg caacacgtgc gcgccgtcgc gggggagccc ttcgagagcc tcctgtgccc
     7441 ccgcggtgag cgggatctca cgccacttcc cggacttcgt gcgctctttg tcggtcgaga
     7501 ggattttcat cgcctcgcgg ccgatccagt cccaccggag cataagtccc tcggcgcgcc
     7561 gcaggcccgt gttcgcgtag agccgccacc agtgcgcgtg cagcgggttc ggctgcggcc
     7621 cctcgccggc gtttacggtt gcccggcagg cagcgtagat gcgggcgagc tcggcagccg
     7681 tgaagaaacg agggggcttt gagtcgagat tcttcggccc gaccacgcgc gctatagggt
     7741 tcgcctcaag atccttccac tctaccgcct tgttgaggat tgccttcaac gtgcggatct
     7801 ctttggtgag cgtctgtgac ctgggcggag tttcggccgt gccgcgagca cgcttgtatt
     7861 cttccgcccg tttgggtgtt atctcatcca gtcggtcccc ggagaacgct gggataaggt
     7921 actgctcgag gatctgctcg atgcggtagt ggcttgttgg aaattccagc gcgtgccact
     7981 tgacgtactc ctttgcgaag tcgtcgaaca gtggcgcgcg gcgctggatg agcttgtgcc
     8041 cgggctgaag ggctagttgc ttgttgcgga ggatgcggcg cgtttcgtcc ttcgggaaga
     8101 tgccgacgcg gccaagcggg tcgcgatgtc gcgtcccttc cacgtctcgc cagtcgagca
     8161 cgtaggactt accaatgatt cgaagtctcg ccatgtccgc tccatcgcgc gcgctagatc
     8221 ggtgcgccgg tatacaacct taccgagcca cgggaaaccc atgatgccct ttcccgcggc
     8281 gcacttatcg aactgagaga ggctcacgca gcagtagtgc gccgcctcgg cccgagtgaa
     8341 gtagtctttg cctgagagat ccagcggtcg gcgttggggc gcctctgtcg ccccgcgcgg
     8401 agcggtttct gtttcggtag ggcgagggtt cacgtttgtc actcctgttc agcgagtcgt
     8461 ccccttcgct tggaccccgc tggtggtcga cctgcggccc atttgtgata gccctccatg
     8521 aggatgtgga tgcgcccgtc cggggctcga tgccacacct tgtcctcttc ccatacaccc
     8581 tcctctatcc tgcttcgaat cgagctttca gagtacccgg tgagctgcgc ggccagcgcg
     8641 attgtgacac tgcgcgccgg tgcgacgacg atctgctcta cgattctatt cgtcatattt
     8701 ttgcctcgga ttttgccttc gcctgcaacg ccttcctacg gtggactcgt gatccggtgg
     8761 gatgcgcgcc ctcgggacac gatcgggtac ccagcaaggc cgatccgacg gccggcttga
     8821 gctcgtcgag cgagaggatc agatcgctca tagaaagctg ctcctgcacc gtgttttcgc
     8881 tttcccaact ggcctgcgat gctctcccag attcgtgggg tcttctaagg atctccttgc
     8941 cctgtgtatc gtcggtggcg agacggccat cccgataccg aatggccctc cgcccatttc
     9001 aagtagccct gcagatctac cagtatgcgg ccgtccgggg cgcgtctcca ctgcttgaac
     9061 tcctcccaaa cgccgtgatg aattttcgct tcgatcgcgc gtactgtgta tccggtgagc
     9121 tgacttgcaa gtttaattgt cacgtagcgc atcggcgtta gtagcgggcc aacgaatccg
     9181 attgtgttct ctggggcggc catacttttc tcctctggtt ggggctttga gttcagtgtg
     9241 ccggagagca aacatagaac ggcccctagg catagcgcca gtgttatgct atcgggatga
     9301 tgcgccagtc caacggcgcg caaaaatgta ccgcactgtg tgacgaacgg gccgccgcgc
     9361 attgccaatc ggagcaatca aatgagcgcc gagcagcgaa atctttacga cttaattgga
     9421 atgtcgcgcg atgccagcca agagagcatc gtgcagcggt gcttgagcct cggtgaggaa
     9481 ctaggaccgg acaagaacgc gggtaacttg cggaccgcgc tcctgttcgc acagatcgag
     9541 aaagcctacg aaatcctcgg cgacccagtg aggcgggccg cttacgatgc ggagttgcga
     9601 gaaaaagaag ccgggtatgc gtccagtgtt ggcccccgta tcgaccgagg tcccgactat
     9661 ccagccaatg cccggcggtg gggacagttc tctaggagca agttaatagc catcgcggta
     9721 gtagttattg ctgccattgc tcttaccggt gggatagccg cgataagcgt aagaaataaa
     9781 gaggcggcga gggctgcgga agcccagaga gtcgcagagg agacggcaag gcgcgcggcc
     9841 gaagagagag caagagaact tgaggcgcgc gccaaggccg ccgaggagag agtgagaatg
     9901 gccgcagtga gggctgcgga agagagcgcg ccgcgagtaa ggagaatgac cgcttcagag
     9961 ctgcgcgcgg ataaagagga tctggcgcgc gtggaaaacg tgcgcgctca gtgtgaggcc
    10021 gcaggtaggt cggttgaaag aagtaggggt ggcggtacgt tcagcgatag cattgggaca
    10081 tttgtaggcg cacaactcgc tataccaatc tgcatgcagt ccgaacgggc ggtgaaagac
    10141 aggctgcgct acgaggcgtt gcgtagcgaa gacaccgctg ccgcgatgaa ggagcttgaa
    10201 ttgcagcgcg ccgctcgcat cgagcgccag cggaaccgcc cagtaatcac taattgcaca
    10261 acggaccggt atggaaacac gaactgtata tcgcgctaaa gcgcgcggca gtggaacctc
    10321 accctgggcg gcgggcggtt cagcccgcgc ggccccacga taagcgacga gcagaaatga
    10381 cgatgcagtt catcacaacg ctggcggaat acacgaggcc gtggaagctc gtgacgcttg
    10441 cgatcggggt cgctttgctg atcgtcggtt ctttctacta tcaggccccg gattgggaca
    10501 tacccatcag cctcataatg gcgatcctgg cctacctcac ggcgccgtgg agtcttcgtg
    10561 tgatccttga gcgtaggtgg cggctctggc cggcgatgct gctcgcgacc tggttcaccg
    10621 tcgacggaag ctattggctg tattggagct tcaaggaccc cgtcgcgctc gagctgatgc
    10681 gcgaggcgaa tttcttcgcg tcgctcgccc tctatgggat gtgcggcgtc gcctggctct
    10741 atcgcggcag cctccgccaa ctaattttgg agattggagc actgcgccgg cgcgcggcca
    10801 gcgctcatcc cgaccgaagg ccccccagat aggagtcagt agcgcccatg aggtataccg
    10861 aggcacaggc gcaagagttt gatgcggctt accaaagcct tacgccgcac cttcgcgtgg
    10921 cagagggtcg ccttctggaa ctcgtgcagc gagctatcgc gcgcattgat gataaaaggc
    10981 tagttcgcgc acaggttcgg ggaactagaa tcaaggcgct cgatagtgtg cgccgcaagg
    11041 cggaggagca tggagtaagt ccaaaagaag cgtgtctttc gatcggtgac cttgttgggg
    11101 caagggtagt atgcaatacg acagaagatg tgcgtcgatt caaggaacta cttcgtcgag
    11161 agctgccctc tggtgaaccc atagaagaag acgaccacat ggctaatccc agaacatctg
    11221 gttaccgcgg gcttcacctc aagttcagaa ttaatattgg agaggggctc atacccgtac
    11281 tcattccgtg cgaggtgcag ctacggacac ttcttcagga tagctgggca gagctggtcc
    11341 atagcgatat ttacaaagag ggcgcaacgc tgcccgaaga cttgcgcgga agaactcagg
    11401 atttggctgg cgtgctcgcg tcggcagacc agatcgcaag cagaatccgc gcgcgcgtta
    11461 tgcaggagac gaaagtcgaa ccagaatcta tacgcctcga taccgtgacg aaggagggcc
    11521 ttgcctacgt gtttgcagag gttttcggcc gcacgccccc cgattatgta gttcaggcag
    11581 cctatgaagc gtgcttagat gcggggttag cggcagtatc ccgtttgcgg ggcaagctgt
    11641 ccgagaagag ttttatagat gcagtgacta aggcttatcg agatgaactt cgccttggat
    11701 tcgatattag taccgaggac attttcaccc ttgcgccggg ggcagcgaca aaaggggata
    11761 aggcggcggc caagaaggcg cgcagattgg cgagagcgga tcgtagcgaa atcgaaacgg
    11821 tttggcgaaa tcaagtattg ggcgagctgc ctgataattg ggagcgtttc cttgaagaac
    11881 ttgaagcgaa gcacgttgac gtggaggcag tagccgaagc gctgggcgtc gcatctgcgt
    11941 gtaccatctg cggtgcgaca atagttgacc actattcgtt tttcgaggcg gctgctcgtc
    12001 attacggggt ggacgccgag tacgaccacg acgttatcca agcgctgagc aacgcaacag
    12061 attgttgggc ggacgacgac tatccgaatc tatgctccta ccacggctac aaggccagca
    12121 aggatgacta acaggcgcgc gaggctaagg gagcagcatg cttccatgct catagcaccg
    12181 atgcgattga gaagtgcctg cgtcggggag tcggtggcta tcgagagact gagtgggcgg
    12241 caggcgacta agctacggtt tgcttacgcc gggtctcgaa caaaggccca ctctctgagc
    12301 tgcttagtca attcctcggt tcgccgtttt gtcgcctcct ctaaagctaa cgaatgggcc
    12361 attgggtagc ctgaaccacc ctcccataga aacacaacga gatccgcgtt cgcgttcgcg
    12421 aagtcgtgcc atttatcttg aacctctctg aataatgtct ttgcgcggtc gtagccgacg
    12481 aatacccgcc gaagttcgtt ttccactctt gcaagttcct ggcgagcgct gtcgagttta
    12541 atgcttgctt tgtttgtcag ctctgcctgg ctcagccccc aatcggtaaa aagagcccgg
    12601 agaagcagtt ctcccgttgc gtacaggaat gattggcacc aattcaagca agccaatacc
    12661 tctaagtcat caatggttac gctagtggcc gactcgtggc agaagatatg tcttagctca
    12721 aacgctcgcg atacatattt gaagcattga tcagggtaag gcagaactag gtcgtgcgcg
    12781 ctctgagctt ctgccatgcc agacttagga gttcgtctca gtaggtcaag aaaatccaca
    12841 ccgagcagac cattcattac gcgctgaata tctccgaggc tgcttaacga caacgagtgt
    12901 gcaactacct ctccagagct tacggccttc ccttcgattg ctcgaacggc gcgccagtcg
    12961 attctaaggt ctctaatgag gggttcgatt cgactcgcgt agggctctcc ggcgtcaagc
    13021 agctcagcgt aagcggcacg gaagtaagat tccaccgcag cgattgcacc gattgggaaa
    13081 tatctcagca cctcgtcacc agctttgaaa ctccccgcct cgaaggcgct cttaagggcc
    13141 gaaatacggc cataatagat actggcgcgc gttccgtact tctctcgggc tttgatttcc
    13201 agcaggtcct gaacggtcct gttcttagcc atgtgcgctc cttaccctaa cagagtcaat
    13261 tcgtgaaacg aggtgtgcta ttctaaaaag gttagccccc gcgcatcggc tttggaggcc
    13321 gatgcccata ggtttctctc gggtcacgct ggcggttgga cggcctgcgc tatgggcgag
    13381 agtcgcctat ggtgggggct aattgttgtt ctctgcgccg gagattctgt cgtttcttgt
    13441 tgaataccga ttgcaatttt attgcgcgca gtcgtcctga ataggtaggt ggcttcaaaa
    13501 aacctaccta gaaagcttat acaaggggca ggcaggtttc tgtgcaggcc gcgggtgggt
    13561 atgcataccg ctgccggcac acaagcagca gctatttttt gggaggtggt tatttctccg
    13621 gggttttggg cgcgccgcct ttggtgtccc aatttgctat cttcgcaatc tcttcgatgc
    13681 cttgctcatt tggactgcca tcgttcttga cgagagcaaa cttttccgca ttgcgttgta
    13741 gccattctat tagggcttgc ttgaccgact tgccgcggaa agattctggg tgggtgctaa
    13801 ccgcgcgcca cgcttcgatc gccgcggcta gcttcaagga aaagtgcgcg tgatcgcggt
    13861 taaggcaatc ctgctctgtt aatgacacaa ttagcttggc ggcgtcgtga ctcgggcgcc
    13921 aatgacgcgc agcgtagcga ctgataagct catcccactg tgctggagat agcccaagca
    13981 gtgtggcagc atcgagctgt tctattgcca agaactccgc ttttctgtca tccctgctcc
    14041 aatcgttcac attctcaggg agcggttctg cgcggtccca ataatgggag ttaaacaatg
    14101 tcaatagggc atttgagcgc ctgtcgccgt cgttaagctc gagaacttct ggtaatccct
    14161 cataacccgt gtcgtagatc ttgaaatccg tggcgcgctc taatagggca attatgtagt
    14221 cgcacgcctc gggtatcttc atctctcgtt gtatacagag caaggcaatc agttgcttaa
    14281 gacggatcaa atctttcgac gttacgatct tcgcaggttt ctcgtcgttg ctgctcatgg
    14341 tttgcgcccc ctttggcgcg gctcgtattt gaatgctacg ccgccaggga aatagtatct
    14401 cggttttttg ccccggcggg ctaggctggg ttgacgaacc tttatttctg ccggtggacg
    14461 ctcggcaccc cccacggaca tggtgcggtg ttttcccccg cgaagcactg atgcgatcga
    14521 g
//
LOCUS       DBNGWZ010000470        12676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1701158, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000470 DBNGWZ010000000
VERSION     DBNGWZ010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1701158"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="AC16CX_14480"
     CDS             <1..1004
                     /locus_tag="AC16CX_14480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013748.1"
                     /translation="YLADRFGAKRLFFLGMLGWSVMSLAFGLIHSFWLAVANQLVAGV
                     FRALLFAPGLALLASSFGPERRATAMSFFWVGCFCGNIVLALLGPFLAARYGWRGAYM
                     FFAALGFVAVFGYYLSPPAAPHGAAQRFAVADALQLLRHGILWICAAIQFVRFAVATA
                     FNFWLPSLLVSERGFSLQAAGLAIAMASALTALGNPVGGYVSDRTRNPPLVIGVSLAV
                     LACTSALLVLVDSIAMVWVVVAVNAIFVGLYFGPLFQVPLEVLGARAAGTATGFGNFF
                     ANVGGLLAAYALGAIKDHAGSFEWGFLGISVLCIAGVALSVALARARAAAAAPATQR"
     gene            complement(1153..2805)
                     /locus_tag="AC16CX_14485"
     CDS             complement(1153..2805)
                     /locus_tag="AC16CX_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013749.1"
                     /translation="MAELTGSEILARSLKNEGTKEFFFIMGGPMMLAESSCAKEGIRP
                     IDVRHEQAAALMAQAYSRLTQKPSVCMAASGPGVINLTTGVANALIDCAPVVAVGGSS
                     AIRQFGRQVFQEIDQLAIMKGCTKWADRVVNLKRIPEMVNTAFQKAMGGKPGPVYLDF
                     PADILYDKIPESEVDWGLSGRSLLRPRPMGDPARVDELIAALSKAKQPIILSGSGVIW
                     SQAWTDLQAFVEKAGIPFYTTPQGRGVLPDDHPLSFLTMRSTAFRDADLILIIGTRMN
                     YIIGHAAPPRFAANATIARIDIDADEIAASPRKVDVGIVGDCKAVLQQLLAAMPGRVD
                     ESRFADWRKKLGEGEAAKRKETGGGMAMNATPIHPLRLCEEVKNFMRRDAILVVDGQE
                     ILNYGRQSMPTFVPAHRLNSGPFGMMGVGLPFGLGAKVACPDKQVIVVHGDGSFGLNA
                     MELDTAVRHKIPVLVVISLNGGWTADPKREKVGRDLGFTRYDKMAEGLGCYGEYVEKP
                     EDIGPALQRAQKKVDQGMVALVNVKTDDKARAATVAFSSHTT"
     gene            complement(2892..4121)
                     /locus_tag="AC16CX_14490"
     CDS             complement(2892..4121)
                     /locus_tag="AC16CX_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3013750.1"
                     /translation="MTDTNAVAPGCLNGVRVLDFTQFEAGTSCTQVMAWLGADVVKVE
                     NPAGGDPGRRTGATRPHRDDPYFLNYNSNKRSITVNLKSPRGLEVVKNLARKADVFIE
                     NFAPGAIERLGLGYNVLSDLNPGIIYGQVKGFGEGSPYENNLAFDMIAQACGGTMSIT
                     GQADGPPAKPGATLGDSGTGMLMAISIIASLYKRKETGQGVRLGVGMQDAMLHYIRNS
                     FGVTTKTGKAAPRAGAKTIEGGNPPCNIYPCAPGGPNDYIYVYTSRANPQHWPRLLEA
                     IGRKDLIGDKRYDTREARVEREAEVDAIIGEWTRKHDKYEAMRVIGAAVPAGAVRDTL
                     ELLNDPDFQERGIIQTIQHPTNGPFKMAGWPVRFNGKPPVVKPAPLLGQHSSEVLKDW
                     LGMSEEQIAALRAEKVI"
     gene            complement(4377..5597)
                     /locus_tag="AC16CX_14495"
     CDS             complement(4377..5597)
                     /locus_tag="AC16CX_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3013751.1"
                     /translation="MDQEILKREDRRRYSLSQQELERRWKAVRERMAAKGMSHLVVQS
                     QQRYVGGYFRWFTDLPGANYHITAVFPLDEDMTVISHGAGAPAPASGPPEWALRGVKE
                     RINTPAFPNVWWEDAWDADKVVEAIKRKKPGTVGLVGLGNMSAALYENLKKGLPGVNV
                     VNATDLVDEVRMVKSEEELKLHRDAAYLHEMSWDLAKKAIQPGRIAREVIAEIREAQY
                     LAGSEEQQIDISFGRPGTAFYSQSSWGNTFVRRTLQEGDVVNILIESSAAGGYWYDLR
                     RFLFIGKPPGELQEAYAIVKEARSLLATNLKPGVVPAAALEESNLFLKGRGSPPEARV
                     AGHGQGLDLVERPVVRPEETARLQAGMVLSIHPTAKVKNVAACISDTYVIGGSGAVPI
                     YQNLFEDDELVAVS"
     gene            complement(5686..8448)
                     /locus_tag="AC16CX_14500"
     CDS             complement(5686..8448)
                     /locus_tag="AC16CX_14500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3013752.1"
                     /translation="MHQRLAKIHLDLKVNGEERQVLVEPQQTLLGVLRDGLGLTGTKQ
                     GCDDGTCGTCVVTVNGAMARACRVPIDRVRGKDILTIEGLGTRGRLHPLQAAFINAGA
                     VQCGFCTPGAIMAAKALLDRNPFPTRAQIAKALGSNLCRCTGYVSILEAVTRVADLGR
                     GVVLPEPPAWGKVPGQADARDKVLGTALYAADLTMPAMLHGAILRSPHPHAEILHIDD
                     SEARALPGVVAVVTAKDVPGLNRYGRAIKDQRVLADGRVRQIGEAVAAVAATSPEAAT
                     QALSLIRVNYRPLPPILDPAEALKEGAPLVHDSGNLLDNREIHWGDVEAGLAQADIVV
                     DETYTTPFCEHAYLEPESALAYLDNEGRLVLRSATQHSHLHRETVAETMGLPEEQVRI
                     HPTVVGGAFGAKTDVSCECIVALLVLKTGQPVKIVYSRAESFASTTKRHPFRMRCRTG
                     ATRDGRLTALAADLLADTGAYASAGPSIMVRAFVSLVGPYHFPNVSIHGRVVYTNNTL
                     AGAMRGYGAPQVAFAIESQMDIMAAKLRIDPLEFRLMNRRRSSAAEATPQDREQEAAY
                     RETVEAVRPYYEQALRARQASVPGDGRWRRGVGIASMRYGIGYADRQSATATLELETD
                     GRVRLFTGAMDMGQGTDTALKLIVAGELALPLESVSVVSGDTDVTPDTGPSSGSRVIY
                     TDGNAVKEAAVLLREAILSTASELLELSYEKLELRDGRVAPRSETGISGSTISLAEVA
                     RARLSAGAPLRFEGAFHPVPVRDTLSGRPSPYLSHVSATHVAEVEVDMEEGTVRVLRV
                     VAAHDVGRVIYSLGLKGQIEGAVSMGMGYALKEEFHPGETTGFKQYRIPTARETPEVI
                     PLTVEMVDPSASIGAKGAAECATVAVAPAIVNAIADATGIRVHHLPATPPRLLALIAG
                     KEAKLI"
     gene            complement(8527..8829)
                     /locus_tag="AC16CX_14505"
     CDS             complement(8527..8829)
                     /locus_tag="AC16CX_14505"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR3013753.1"
                     /translation="MNSKNQASHGSRVTGVKAPARVVFTGDFSDRYTGGVKEFEIDAK
                     NLRGVIKALDELYPGLGEHLEKETTVAIDGEIHEVGYYQPVRPGCEIFFIPKIEGG"
     gene            complement(8819..11098)
                     /locus_tag="AC16CX_14510"
     CDS             complement(8819..11098)
                     /locus_tag="AC16CX_14510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3013754.1"
                     /translation="MPATIETEERKLKVVGTRPVRPDGVPKVTGLARYGADYSLPGML
                     WGKILRSPHAHARIRSIDTSKAQALPGVKAVMTSADLPEQKFEYVGPERVAVNFWHMT
                     RNVMAREKVLYEGHAIAAVAAISKTVADEALRLIEVDYEVLPHVIDVEEAMRPDAPLL
                     FEDMITRGVEPAPDKPSNISKRLEFKIGDLEAGFAAADEIVEMSFKTAPVHQGYIEPQ
                     ACLARFDTDGQGEVWASSQGHFVIRALTARLLGMEIGNLRVHPAEIGGGFGGKNVPYV
                     EPVALALARKTGHPVKIVMSREEVFKACGPAAGSVMTVRIGVRKDGKIVAADGVFKFQ
                     AGAFPGSPVMNACMCAFAPYDIPNVRSVGYDVVCNRPKTAAYRAPGSPISAYAVESVM
                     DVVAARIGMDPLQFRLNNIARPGTPTVWGPKLAHEGFAETIQALMKHPGYAAPLGKNQ
                     GRGVASGYWFNNGGESSATVHVNEDGTVLVATGSSDLSGTRASMAIMAAETLGIDYDK
                     VRPIVADTASIGYTHLAAGSRTTFATGIAVVEACKKVIGELRARAAMIWDVSAEDVIW
                     EDGHARPAGKSKGRFEPLSLKAIAAKRAVTGGPISAEVSVNAGGVAPGFATEFCDVEV
                     DPETGQVKVLRFVAAQDVGRAIHPGYVEGQIQGGAAQGIGWALNEEYIYDEAGRLANA
                     GFLDYRVPVASDLPRIEAVLVEVPNPSHPFGVKGVGEANIVPSLAAVSNAVSRAIGRR
                     MTELPMSPPKILKAVNSEQ"
     gene            complement(11101..11577)
                     /locus_tag="AC16CX_14515"
     CDS             complement(11101..11577)
                     /locus_tag="AC16CX_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3013755.1"
                     /translation="MAKHHVTATVNGDVVEFLCDTRETLLEVLRDELGLTGTKEGCVT
                     GDCGACSVAIDGRLMCSCLVLGVEAEGKSIQTIEGMAAGTELHPLQRKFLEHAALQCG
                     FCTPGILIAARSLLERNPDPTEEEVRYWLAGNLCRCTGYHKIVDAVLDAAREMRRG"
     gene            complement(11577..12440)
                     /locus_tag="AC16CX_14520"
     CDS             complement(11577..12440)
                     /locus_tag="AC16CX_14520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3013756.1"
                     /translation="MRYEAPKTLGAALALLAGANGQARVLAGGTDLLVGMRSGRFEPE
                     VLIDIKGIPEMTSIVAENGGFRFGAAVTCMELVEHEAFAKAWPGVIEGIRLIGSIQVK
                     GRATVGGNLCNASPAADSVPALIAAGASVSIVGPSGRREARVEDIATGPGKISLAKGE
                     IVASFLLPRRPPHSGDAYLRFTPRTEMDIAVVGAGVNLTLDGSGVCSQARVSLGAVAE
                     RALLVPEAAAALIGTKIDAAALKRLAAAASAACRPIDDKRGTREFRIKIAGVMARRAA
                     EIALARARKRN"
BASE COUNT         2097 a         4288 c         3844 g         2447 t
ORIGIN      
        1 gctatctcgc ggaccgcttc ggcgcaaaac gcctgttctt cctcggaatg cttggatggt
       61 ccgtgatgtc gctcgccttc ggcctcattc actccttctg gctcgccgtg gccaatcaac
      121 tggtggccgg cgtgttccgc gcgctgctgt tcgcgccggg gctggcgctg ctcgcatcgt
      181 cgttcggacc cgagcgccgc gctaccgcga tgagcttctt ctgggtggga tgtttctgcg
      241 gcaacatcgt gctcgcgctg ctcggaccgt tccttgcggc gcgctacggc tggcggggcg
      301 cctacatgtt cttcgccgcg ttgggcttcg ttgcggtgtt cggctactac ttgtcgccgc
      361 ctgcggcgcc tcacggcgcg gcccagcgct ttgccgttgc cgatgccctg caactgctcc
      421 ggcacggcat cctgtggatc tgcgccgcca tccagttcgt ccgctttgcc gtggccaccg
      481 cgttcaactt ttggctgccg tcgctgctcg tgtccgagcg cgggttctcc ctgcaggcgg
      541 ccgggctcgc catcgcgatg gcctccgccc tcaccgcgct cggcaatccg gtggggggct
      601 acgtctcgga ccgcacgcgc aatccgccgc tcgtgatcgg cgtctccctc gccgtgctcg
      661 cctgcacctc cgccctgctg gtgctggtgg attcgatcgc gatggtatgg gtggtcgtcg
      721 cggtcaacgc gatcttcgtg ggtctttatt tcggtccctt gttccaggtg cccctggaag
      781 tgctgggtgc ccgcgcggcg ggcacggcca ccgggttcgg caacttcttt gccaacgtgg
      841 gcgggctgct ggcggcctac gcgctgggcg cgatcaagga ccacgcgggc tcgttcgagt
      901 ggggcttcct cgggatcagc gtcctgtgca ttgccggcgt ggccctgagc gtcgcgctcg
      961 ctcgcgctcg cgccgctgcc gcagcgccgg cgacgcaaag atgaaggatg aagctacgtt
     1021 gtcgtgatct gcttcaacaa ttttcttagt ggtttgtccc ctaagcgctc ttacattagc
     1081 gaatcaatgc attgcgcagg tccgacccga aagtggtgcc acggctccct cgagccaagc
     1141 ggcggcttcg tgtcacgtcg tatgagatga gaacgctacc gtggcagcgc gcgctttatc
     1201 gtcggtcttg acgttgacga gggccaccat gccttgatcc accttcttct gcgcacgttg
     1261 cagtgcgggt ccgatgtctt ccggtttctc cacgtactcc ccgtagcagc ccaacccctc
     1321 ggccatcttg tcgtagcgcg taaagccgag atcgcgcccg actttctcgc gcttcggatc
     1381 ggcggtccag ccaccgttca gactgatcac cacgagcacc gggatcttat gccgcacggc
     1441 cgtgtccagc tccatcgcgt tcagcccgaa ggaaccatcg ccatgaacga cgatgacctg
     1501 cttgtccggg cacgcgacct tggcgccgag cccgaacggc agacccacgc ccatcatccc
     1561 gaacggtccg gaattcagcc ggtgcgccgg cacaaaggtc ggcatggatt ggcggccgta
     1621 gttcaggatc tcctgcccgt ccaccaccag gatggcgtcg cgccgcatga agttcttgac
     1681 ctcttcgcac aaacgcaagg ggtgaatcgg cgtcgcattc atcgccatgc cgcctccggt
     1741 ctccttgcgt ttggccgcct cgccctcgcc gagcttcttc cgccaatccg cgaaacggct
     1801 ctcgtccaca cggcccggca tcgccgccaa gagctgctgc agcacggcct tgcagtctcc
     1861 gacgatcccg acgtccacct tgcgcgggga cgctgcgatc tcatcggcat cgatatcgat
     1921 ccgtgcgatc gtggcattgg cggcaaagcg cggaggcgca gcatgaccga tgatgtaatt
     1981 catgcgcgtg ccgatgatca gaatgaggtc ggcatcgcga aacgctgtgg agcgcatggt
     2041 gaggaaggac agcggatgat cgtcgggcag cacgccgcgg ccctgcggcg tcgtgtagaa
     2101 cgggatgccc gccttctcta cgaacgcctg caggtcggtc catgcctgcg accagatcac
     2161 gccgctgccg ctgagtatga tcggctgctt cgccttcgag agcgcggcga tcagctcatc
     2221 gacgcgcgcg ggatcgccca tcggccgcgg gcgcagcagc gagcgtccgc tgaggcccca
     2281 gtcgacctcg ctttccggaa tcttgtcgta aaggatgtcg gccggaaaat cgagatagac
     2341 cggcccaggc ttgccgccca tcgccttctg gaaggccgtg ttcaccatct cgggtatccg
     2401 cttcaggttc acgacgcggt ccgcccactt ggtgcagccc ttcatgatcg cgagctggtc
     2461 gatttcctgg aacacctgcc tgccgaactg cctgatcgcg cttgagccac cgaccgccac
     2521 gaccggcgcg cagtcgatca gcgcgttggc gacgccggtc gtgagattga tcacgccggg
     2581 accgctcgcc gccatacaga cgctcggctt ctgggtgagc cggctgtaag cctgcgccat
     2641 cagcgcagcg gcctgctcgt gacgcacgtc gatcgggcgt atcccctcct tggcgcacga
     2701 actttcggcg agcatcatcg gcccgcccat gatgaagaaa aactccttcg tcccctcgtt
     2761 cttcagggag cgcgccagga tttcagagcc tgtcagttca gccatcgtcg atactccttg
     2821 ttcaaatacg aggtagtgag ccgtcggcgc cgcctcgctg aaaatagcgc ccccaaagca
     2881 cgtcggcgac cctagataac cttctctgcg cgcagcgcgg cgatctgctc ctcactcatg
     2941 ccgagccagt ccttgagtac ctccgaactg tgctggccga gcaagggcgc cggtttcacc
     3001 acgggcggct tgccgttgaa tcggacgggc cagcccgcca tcttgaacgg cccgttggtc
     3061 gggtgctgta tggtctgtat gattccccgt tcctggaaat cgggatcgtt cagcagttcc
     3121 agtgtatccc gcacggcgcc cgcaggaacg gccgcgccga tcacgcgcat cgcctcgtac
     3181 ttgtcgtgct tgcgggtcca ttcgccgatg atcgcgtcca cttcggcttc ccgttcgacc
     3241 cgcgcctccc gcgtgtcgta gcgtttgtcg ccgatcagat ccttgcgccc gatcgcttcg
     3301 aggagtcgcg gccagtgctg cgggttggca cgactggtat agacgtaaat gtagtcattc
     3361 ggcccccccg gtgcgcacgg atagatgttg cagggaggat tgccgccctc gatcgtcttc
     3421 gctccggcac gcggggcagc ctttccggtc ttggtggtga cgccaaacga attgcggatg
     3481 tagtgcagca tcgcgtcctg catgcccact cccagccgca caccctggcc ggtttccttg
     3541 cgcttgtaga gggacgcgat aatactgatc gccatcagca tcccggtccc cgaatcgccc
     3601 agcgtcgctc ccggcttggc gggtggcccg tcagcctgcc ccgttatgct catcgtgcca
     3661 ccgcacgcct gggcgatcat gtcgaaggcg agattattct cgtacgggct accctcgccg
     3721 aagcctttga cctgtccgta gatgattccc ggattgagat cgctcaagac gttgtagccc
     3781 agacccaggc gctcgatggc cccgggcgcg aagttctcaa tgaaaacatc ggccttgcgc
     3841 gccaggttct tgacgacctc gagcccgcgc ggggatttca ggttgacggt gatcgagcgc
     3901 ttgttggagt tgtaattcag gaaataagga tcatcccggt gcggcctggt ggcgcctgtc
     3961 cgacgcccgg gatcgccgcc ggccggattc tccaccttca ccacatcggc accgagccac
     4021 gccatgacct gggtgcagga ggtgcccgcc tcgaactgcg taaagtccaa tacccggacg
     4081 ccgttcaggc aacccggcgc cacggcgttc gtatctgtca tcggttgctc caacgatggg
     4141 gaaataagaa atgggaatag aaatgcgagg cgcaggcaat cttagcgtat ggtggcaatt
     4201 ccatctatct tccgatgcgc cgcattctga gcttcccgcg aaatttcgtc gtccgggaag
     4261 cggagtgcaa cgtcaactga ctggaaggag agacatgacg atgagcctca tggtgaggcc
     4321 gattacccgg ctcaagcgct accctccttc gaccttcgga atgaaaaaga tctcgctcaa
     4381 ctcacggcga cgagttcatc gtcttcgaac aggttctggt agatcggaac ggcgcccgag
     4441 cctccgatca cgtaagtgtc cgaaatgcag gccgcgacgt tttttacctt tgcggtcgga
     4501 tgtatcgaca acaccatgcc ggcctggagc ctggccgttt cttccggacg aacaacgggc
     4561 cgctccacga gatccaggcc ctggccgtgt cccgcgacgc gcgcctcggg cggagatccc
     4621 ctgcccttca ggaacaggtt gctttcctcc agagccgccg ccgggaccac gccgggcttg
     4681 aggttggtcg ccaggagact ccgcgcctcc ttgacgatcg cataggcctc ttgaagctcg
     4741 ccgggaggct tgccgataaa aaggaacctt cgcaggtcgt accagtaccc gcctgccgcg
     4801 ctggactcga tcaggatgtt gacgacatcg ccctcttgca gcgttctgcg cacgaaagtg
     4861 ttgccccagc tcgactgcga ataaaacgcg gtgcccgggc gcccgaagga aatatcgatc
     4921 tgttgttcct cgcttcccgc caaatactgg gcttcccgga tttccgctat cacctcccgc
     4981 gcgatcctcc ccggctggat cgccttcttc gccaagtccc agctcatctc gtgcaagtag
     5041 gcggcgtccc ggtgaagctt gagctcttcc tcgctcttta ccattctgac ttcatcgacg
     5101 agatcggtcg cattgacgac attcacgcct ggcaggccct tcttcagatt ttcgtacagc
     5161 gccgccgaca tgttgcccag tcccaccaaa ccaaccgtgc ccggcttctt gcgcttgatc
     5221 gcttccacca ccttgtccgc gtcccacgca tcctcccacc agacgttcgg aaaagccggg
     5281 gtgttgatcc gctctttcac gcctcgcaac gcccattcgg gcggtccgct ggccggtgcc
     5341 ggcgcgcctg cgccgtggct gatgacggtc atgtcctcat ccagcggaaa gacggcggtg
     5401 atgtggtaat tcgcgcccgg caagtcggtg aaccagcgga aatagccgcc gacgtatcgt
     5461 tgctggctct gaacgacgag atggctcatt cccttggcgg ccatccgctc acgcacggcc
     5521 ttccatctgc gctcgagctc ctgctgggac agcgaatacc gtcttctgtc ctctctcttc
     5581 agaatttcct ggtccatgat ggatgtcctt tcgtcagaaa gcgcgataac gattgcttcc
     5641 acaacacttg ccatgaattc gctgaacctg gacggggccg gcgaactaaa tgagcttggc
     5701 ctcttttccc gcgatcaggg ccagtagacg aggaggcgta gccggcagat ggtgaactcg
     5761 aattcccgtg gcgtcggcaa tggcgttgac gatggcgggg gccacggcca ccgtcgcaca
     5821 ttcggccgcg cccttggccc cgatgcttgc cgaggggtcc accatctcga ccgtcagcgg
     5881 gatgacttca ggggtttccc gcgcggtggg gatgcggtat tgcttgaagc ccgtcgtctc
     5941 gccgggatgg aattcctcct tgagggcgta acccatgccc atggacacgg cgccttcgat
     6001 ctgtcctttc agccccagag aatagatcac gcgtcccaca tcatgcgcag ccacgacgcg
     6061 aagcacccgc accgtcccct cctccatgtc gacctccacc tccgcaacgt gggtcgcgga
     6121 cacatgcgac aggtagggac tcgggcgccc gctcagggtg tcgcgcacag ggacgggatg
     6181 aaaggctccc tcaaaccgca ggggcgcccc tgccgacagc ctggcccgcg cgacctccgc
     6241 cagggagatg gtcgagccgg agatgccagt ctcgcttcgt ggcgccaccc gcccgtccct
     6301 cagctccagt ttctcgtagg acaattccag caattcggac gccgtggaga ggatggcctc
     6361 cctcagcagg accgccgctt ccttcaccgc gttgccatcg gtatagatca ccctgctgcc
     6421 tgaggagggg ccggtatcgg gggtaacgtc ggtgtcgccg ctcaccaccg acaccgattc
     6481 cagcggcaac gccagttctc cggcgacgat gagtttcaag gccgtgtcgg ttccctggcc
     6541 catgtccatg gcaccggtaa aaaggcgcac gcggccatcg gtttccagct ccagggtcgc
     6601 cgttgccgat tgcctatcgg cgtagccgat gccatatcgc atggaggcta ttcccacccc
     6661 gcgccgccac cggccatcac cggggacgct ggcctgacgt gcccgcaacg cctgctcata
     6721 gtagggacgg acagcctcaa cggtctcacg gtatgccgct tcctgctccc tgtcctgagg
     6781 cgtcgcctca gccgcgctcg acctgcggcg gttcatcaac cggaattcca gagggtcgat
     6841 gcgaagcttg gcggccatga tgtccatctg ggactcgatg gcaaaggcca cctgcggggc
     6901 accgtagccc cgcatggcgc ctgccagggt gttgttggtg tacaccactc gcccgtggat
     6961 cgacacgttg gggaagtggt agggacccac gagcgacaca aaggcccgaa ccatgatcga
     7021 gggacccgct gacgcgtagg cccccgtatc agccagaaga tccgcagcga gggcagtgag
     7081 tcgaccgtcc cgggtcgcgc cagtccggca gcgcatgcgg aagggatggc gcttggtggt
     7141 ggaggcgaac gattcggcgc gactatagac gatcttcacc ggctgacccg tcttcaggac
     7201 gagcaacgcc acgatgcact cgcaggaaac gtcggtcttg gcgccgaagg caccgcccac
     7261 cacggtggga tgaatgcgca cctgctcctc gggcaggccc atggtttcgg ccaccgtctc
     7321 gcggtggagg tgagagtgct gcgtggccga tcgcaacacc agtcgccctt cgttgtccag
     7381 gtaggccagt gccgactccg gctcaaggta agcgtgctcg caaaagggcg tcgtgtacgt
     7441 ttcatcgacc acgatatctg cctgcgcgag gcctgcttcc acatcacccc agtggatttc
     7501 cctgttgtcc agcaagttgc cgctgtcgtg gacaagtggg gcgccctcct tcagggcctc
     7561 ggcgggatcg aggattggcg ggagaggccg gtagttcaca cgaatcaatg agagagcctg
     7621 ggtcgctgct tccggcgatg tcgcggcaac ggcagccact gcctcgccga tctgccgcac
     7681 ccgaccgtca gccagcacgc gctgatcttt gatggcgcgc ccgtaccgat tgagtccggg
     7741 cacgtccttc gcggttacta cggccaccac acccggaagc gcccgggcct cgctatcatc
     7801 gatgtgaagt atctcggcgt gagggtgagg gctccgcaga atcgctccgt ggagcatggc
     7861 gggcatggtg aggtcggcgg catagagggc ggttcccaac accttgtcac gcgcatcagc
     7921 ctgcccgggc acctttcccc acgctggcgg ctccggtaaa acgacacccc ggccaagatc
     7981 ggccacgcgg gtgaccgctt ccaagatgct cacgtagccg gtgcagcggc acagattgct
     8041 ccccaaggcc ttggcgatct gcgctctcgt ggggaacgga ttgcggtcca gcaaggcctt
     8101 ggccgccatg atcgcgccgg gcgtgcagaa accgcactgg acagcaccgg cgtttatgaa
     8161 ggctgcctgc aacgggtgaa ggcgcccgcg ggtgcccagc ccctcaatcg taagaatatc
     8221 cttgcctcgc acgcgatcga tgggcacgcg gcacgcccga gccatcgcgc cgttcacggt
     8281 aactacgcag gttccgcacg taccgtcatc gcacccctgc ttggtgccgg tgaggccaag
     8341 accgtcacgt aacaccccaa ggagagtctg ctgaggctcc accaaaacct ggcgctcctc
     8401 gccgttgacc ttcaggtcca aatggatctt ggctaaacgt tggtgcaccg cggtgcactc
     8461 ctctcctgga tcccgcctcc ggcagtgata ccgctcgccc gctggttaat cccggccttc
     8521 ccgcttctac ccgccttcga tcttcggaat gaagaagatc tcgcaacccg gccgcaccgg
     8581 ctggtaataa ccgacctcgt ggatctcgcc gtcgatcgcg acggtggttt ccttctccag
     8641 gtgctcgccc aggcccgggt agagctcgtc cagcgccttg atgacgccgc gcaggttctt
     8701 cgcatcgatc tcgaattcct tcaccccgcc cgtatagcgg tcgctgaaat cgccggtgaa
     8761 aacgacccgc gcgggcgcct tgacaccggt gactcgtgat ccatgactcg cctggttttt
     8821 actgttcact gttcacggcc ttcagaattt tgggcggcga catcggcagt tcggtcatgc
     8881 gccggccgat cgcacggctg accgcgttgg agaccgccgc cagcgacgga acgatgttgg
     8941 cctcgcccac gcccttcacg ccaaacgggt ggctggggtt cggcacttcg accaggaccg
     9001 cctcgatcct cggcaaatcg gaggcgaccg ggacccggta gtcaaggaag ccggcattcg
     9061 caagtcgccc cgcctcgtcg tagatgtatt cctcgttcaa tgcccagccg atgccctgcg
     9121 cggcgccgcc ctggatttgc ccctcgacat agcctggatg aatcgcgcgg cccacgtcct
     9181 gggcggccac gaagcgcagg actttcacct ggcccgtctc ggggtcgacc tcgacgtcgc
     9241 agaattcggt ggcgaagccg ggcgcgacac cgcccgcgtt gaccgacacc tcggcgctga
     9301 tcggcccgcc cgtcactgcc cgcttggcgg cgattgcctt gagcgacagc ggctcgaacc
     9361 ggcctttgct cttgcccgcg ggcctggcgt ggccgtcctc ccagattacg tcctccgcgc
     9421 ttacgtccca gatcatcgcg gcacgggcgc gcagctcacc gatcaccttc ttgcacgcct
     9481 cgactaccgc gatgccggtc gcgaaagtgg tgcgcgagcc ggcggcgaga tgggtgtagc
     9541 cgatcgaggc ggtgtcagcg acgatcggcc ggactttgtc gtaatcgatc cccagcgttt
     9601 cggccgccat gatcgccatc gatgcccgcg tgccgctgag gtccgaactg ccggtggcga
     9661 cgaggaccgt cccgtcctcg ttgacgtgta ccgtcgcact cgattcgccg ccattgttga
     9721 accagtagcc cgaggctacg ccgcgtccct gattcttgcc gagcggcgcg gcgtagcccg
     9781 gatgcttcat caaggcctga atcgtttcgg cgaagccttc gtgcgcgagc ttcggccccc
     9841 acaccgtcgg cgtaccgggc ctggcgatgt tattcagcct gaactgcagc ggatccatgc
     9901 cgatcctggc cgcgaccacg tccatgacgc tctcaaccgc gtacgcggag atcggcgagc
     9961 cgggggcgcg gtatgcggcc gtcttgggcc ggttgcaaac cacgtcgtag cccaccgagc
    10021 gtacattcgg aatgtcataa ggcgcgaagg cgcacatgca ggcgttcatg accggcgagc
    10081 cggggaaagc gcccgcctga aacttgaaga cgccgtcggc cgcgacaatc ttgccgtcct
    10141 tcctgacgcc gatcctgacc gtcatgaccg acccggcggc cggtccacag gccttgaaca
    10201 cttcctcccg gctcatcacg attttcaccg gatggccggt cttgcgggcg agagcgagcg
    10261 ccaccggctc gacataggga acattcttgc cgccgaagcc gccgccgatt tccgccggat
    10321 ggacgcgtag gttcccgatc tccatgccga ggagccgtgc cgtcaacgcg cgtatcacga
    10381 agtggccctg gctcgacgcc cagacctccc cctggccgtc ggtatcgaag cgcgcgaggc
    10441 aggcttgcgg ctcgatatag ccttgatgca ccggcgcggt cttgaagctc atctccacga
    10501 tctcgtcggc tgcggcgaag ccggcctcga ggtcgccgat cttgaactcc agcctcttgg
    10561 agatgttcga gggcttgtcc ggcgcgggct cgacgccccg ggtgatcatg tcctcgaaca
    10621 gcaggggcgc atcgggccgc atcgcctctt cgacgtcgat gacgtgcggc agcacctcat
    10681 aatcgacctc gatcaggcgc agcgcctcgt cggcgacggt cttgctgatc gcggcgaccg
    10741 cggcgatggc gtggccctcg tagaggacct tctcgcgcgc catgacgttg cgcgtcatgt
    10801 gccagaaatt cacggcgaca cgctcggggc cgacatactc gaacttttgc tcgggaaggt
    10861 ccgccgacgt catcacggcc ttgacgccgg gcagcgcctg cgctttcgag gtgtcgatcg
    10921 agcggatgcg ggcgtgagca tgcggagagc gcaggatctt gccccacaac atgcccggca
    10981 gagaataatc cgcgccgtag cgggcgaggc ccgtcacttt cggcacgccg tcgggccgta
    11041 ccggacgggt gccgacgacc ttcagtttcc tttcctcggt ctcgatcgtc gcaggcatgc
    11101 tcagcccctc cgcatttcgc gtgcggcgtc gagcaccgcg tccacgatct tgtgatagcc
    11161 ggtgcagcgg cacaggttgc cggccaacca atagcggact tcttcctcgg tcggatccgg
    11221 gttgcgctcc agcagcgagc gggcggcgat caatatcccc ggcgtgcaga aaccgcactg
    11281 cagcgccgcg tgctcgagaa atttgcgctg caacgggtgc agctccgtgc cggcggccat
    11341 gccttcaatg gtttgtatgg atttgccttc ggcctcgacg ccgaggacga ggcaggagca
    11401 catcaggcgc ccatcgatag ccacgctgca ggcaccacaa tcgcccgtga cacagccttc
    11461 cttggttccg gtgaggccga gttcgtcgcg cagcacttcg agcagggtct cgcgcgtgtc
    11521 gcaaagaaac tcgacgacat ccccgttgac cgtcgccgtt acatgatgtt ttgccattag
    11581 ttcctcttcc tcgcccttgc gagcgcaatt tcagcggcgc gtcgcgccat cacaccggca
    11641 atcttgatcc ggaattccct ggtcccgcgc ttgtcgtcga tcggccggca ggccgcgctt
    11701 gcggcggcgg ccagccgttt gagcgcggca gcgtcaatct tggtgccgat cagcgcagcc
    11761 gcggcctcgg gcaccagcaa agcgcgctcg gcgaccgcgc cgaggctgac ccgcgcctgg
    11821 ctgcagacgc cgctgccgtc cagcgtgagg ttgacccccg ccccgacaac cgcaatgtcc
    11881 atctcggtgc gcggggtgaa gcgaagatag gcatcgcccg aatgcggagg gcgccgcggc
    11941 agcaggaatg aggcgacgat ctcgcccttc gccagcgaaa tcttgcctgg tcctgtggcg
    12001 atatcctcta ctcgcgcttc gcgcctgccg ctgggcccca caatgctcac ggacgctccg
    12061 gcggcgatca gggcgggaac gctgtccgca gccggtgagg cgttgcacag attgccgccc
    12121 acggtcgcgc gtccctttac ctggatcgaa ccgatcagcc tgatcccttc gatcacgccg
    12181 ggccatgcct tcgcgaatgc ctcatgctcg accagctcca tgcaagtcac agccgcgccg
    12241 aaacgaaacc cgccgttttc ggcgacgatc gaggtcattt cggggatgcc cttgatgtcg
    12301 atcagcacct cgggctcaaa gcggccggat cgcatcccga ccagcaagtc tgtgccgccg
    12361 gccagcaccc tcgcctgccc gttcgcgccc gcgagcaggg caagcgccgc ccccagcgtt
    12421 ttgggcgcct cataacgcat ttagtgcacc tcccccggca aattattgtg caatttcaga
    12481 atcctccatg acccggaccg atcgtcacga ccaagctgcg caacgatcac gggacgcggt
    12541 cgttcctgaa atgtagcgaa tcgtggcgct acaaactttg cgctaaatca aggtttgcgg
    12601 gcttgctgcg atgaacaggc aagaaatgac ctacatcccg cgctattgca gcgcggatca
    12661 ggcggcggtc agcccg
//
LOCUS       DBNGWZ010000471         6101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1701204, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000471 DBNGWZ010000000
VERSION     DBNGWZ010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1701204"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="AC16CX_14525"
     CDS             <1..1016
                     /locus_tag="AC16CX_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR3013757.1"
                     /translation="FTARAFQAVGISARESKSIAELMVRADLQGSEGHGVFRLPQYVR
                     RIKGGAVNIKPNIRVAREAAGMALVDGDNGMGHLVMRFATEKAIEKARSAGVAWVGVK
                     WSNHAGPASLYASMPLEHDMTGLYLAVGNANHLPPWGGLDMLLSTNPIAVAVPAGEEP
                     PIVLDMATTVAAYGKVKTKAQRGEMMPEGWMMDREGLPLTDPRRANEGFLLPIGGYKG
                     YGLALIFGLLAGTLNGAAMGKDVVDFNADDTTPTNTGHVIVAISVEAFQPVAEFKKSV
                     DALIRDIRDSKRLPGVDRIRLPGEGSHAARADRMKNGIPLPAPLAASLNQLASELKLP
                     PLA"
     gene            1121..2092
                     /locus_tag="AC16CX_14530"
     CDS             1121..2092
                     /locus_tag="AC16CX_14530"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3013758.1"
                     /translation="MLFWLTLIAATAWLLWYMVKVPGVSYTGALKPLTSEEQLIAGNL
                     RRHVAAIASREHNLFRPAELEAAAQYIEKALAGVDHAVAAQRIQAGPMEVRNIEIEVT
                     GEGRRDEIIVVGAHYDSVSGAPGANDNGSGVGAIIELARLFRDARPARTLHLVAFVNE
                     EPPFHHSDAMGSRQYSRRSKERGENIVAMFSLETIGYYSDRPGSQHYPFPLGFFYPST
                     GSFIAFVSNLSSRALLHEVIASFRRHAEFPSEGVAAPAFIPGVDWSDHWSFWQEGWPA
                     LMVTDTAPYRYPHYHTAQDTPDQVDYERAARVVIGLFHMLRALAQSE"
     gene            2311..2550
                     /locus_tag="AC16CX_14535"
     CDS             2311..2550
                     /locus_tag="AC16CX_14535"
                     /inference="COORDINATES: protein motif:HMM:NF017269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3013759.1"
                     /translation="MRTLTRMLAIAGIAAMTAGCASAPQTRQGCAEPNYGGAVIGGLA
                     GGLLGAQVGKGSGRTAAAAVGAGTGAVIGSQVGCE"
     gene            2576..3169
                     /locus_tag="AC16CX_14540"
     CDS             2576..3169
                     /locus_tag="AC16CX_14540"
                     /inference="COORDINATES: protein motif:HMM:NF017319.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaC/ttg2D family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3013760.1"
                     /translation="MKILAHVASGLVLVTSLAAQAADTAPDALVKSTVDEVLAVIKQT
                     KDRQTLVELAEKKVLPHFDFKHMTQLAVGKSWTQANAAQQEALERGFRALLMRTYTAA
                     LSQSSGGAKVEVKPSQIKPGDPETVVRTLVNEPGRKPFSIDYRMRNGSGGWKVYDVVV
                     ENLSLVTNYRASFQAEVNRSGIDGLIKAIESKNQQQG"
     gene            3370..4611
                     /locus_tag="AC16CX_14545"
     CDS             3370..4611
                     /locus_tag="AC16CX_14545"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013761.1"
                     /translation="MNEPILPPLATRKKVLLIDDDPDLLSIRTLRLQADGYTVKTVNS
                     SATAMDAIASFQPDLIILDLIMPAISGETLLKQVRGDGCFRAIKIIVNSAKSFECDQK
                     LCLESGADAYLAKPAEHDALVSLIGKLLRDEVTVTFWGTRGSIPRPGKDTLRFGGNTP
                     CVSVELGDDRLFIFDAGTGIVDLGRALVAANKHYKFNLFVSHPHWDHIQGLPFFQPLY
                     LQGNEMVIHGTSHGKLSLREVISGQMDSLYFPITIKEYASRVYFKELEEGDYEIEKLP
                     LSTITLNHPGRTLGYRLGNGNGKSVAYITDNEIFPEGDQYNRRRLAAFLSGVDVLIHD
                     ATYFDEEYPSRARWGHSALSEVLRLADEARVKRLYLFHHDPSHDDDAVDKKETFGRRF
                     FEKRNSDVQCSAAREGVSVTL"
     gene            4729..>6101
                     /locus_tag="AC16CX_14550"
     CDS             4729..>6101
                     /locus_tag="AC16CX_14550"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013762.1"
                     /translation="MRRLYPRTFPRLLAVGFTLVALPLVFALVNNAISVDRVANRSQN
                     AVYQAVQATQNSRRLAQLLTELERTARQMVILDDRSLLDTYAANRRRFEQTVAEFARL
                     PLDAEQKSALDDVVRGETEIFSALSDAGSKRAQLRNVIERFDGLAEQAQAIVARSNAQ
                     IDREVEAMRTTADQAQRITLWQLLALVPVVVFLVIGFSILITRPIRQVDAAIRRLGAG
                     EFRVPVTVSGPQDLEQLGERLEWMRRELVDLEEQKNRFLRQVSHELKTPLTALREGAE
                     LLSEEAVGKLTPEQREIAEILRHNSVELQRLIEDLLSFGASQFRKVTVDLEPVEIRRI
                     VERVTTDQRLAARARGLTLDVAAEDIMVPADPEKLRVVLDNLVSNAIRFSPEGGCVRV
                     VACLDRGALKLDVIDQGPGIPPEERTRIFDPFYQGRHAGAGPVRGTGIGLSVVKEYVF
                     AHGGSVEV"
BASE COUNT         1164 a         1906 c         1998 g         1033 t
ORIGIN      
        1 agttcacggc gcgcgcgttc caggccgtag gcatctcggc gcgggaatcg aaaagcatcg
       61 ccgagctcat ggtgcgcgcg gacctgcagg gctcggaagg ccacggcgtg tttcgcctgc
      121 cgcagtatgt ccgccgcatc aagggaggag cggtcaacat caagccgaat attcgcgtcg
      181 cgcgcgaggc ggccggcatg gcgctggtgg acggcgacaa cggcatgggg catctcgtca
      241 tgcgcttcgc caccgagaag gcgatcgaga aggcgagatc cgcgggcgtc gcctgggtgg
      301 gcgtgaaatg gagcaaccat gccggccccg cctcgctgta cgccagcatg ccgctcgagc
      361 acgacatgac ggggctctat cttgctgtcg gcaacgccaa ccacctgccg ccgtggggcg
      421 ggctcgacat gctgctctcc accaacccga tcgcggtcgc ggtgccggcg ggggaggagc
      481 cgccgatcgt gctcgacatg gcgacaacgg tggccgccta cggcaaggtg aagaccaagg
      541 cgcagcgcgg cgagatgatg cccgaaggct ggatgatgga ccgcgaaggc ctgccgctca
      601 ccgatccccg gcgcgccaac gagggctttc tgctgccgat cggcggctac aagggctacg
      661 ggctcgcgct gatcttcggg ctccttgcgg gaacgctcaa tggcgccgcg atggggaagg
      721 acgtggtgga tttcaacgcc gacgacacca cgcccaccaa caccgggcac gtcatcgtcg
      781 cgatcagcgt ggaagcgttc cagccggtcg cggaattcaa gaagagcgtg gatgcgctga
      841 ttcgggacat ccgcgactcg aaacgcctgc cgggtgtgga ccgcatccgc ctgccgggcg
      901 aaggcagtca tgccgcgcgc gccgaccgca tgaagaacgg catcccgctg cccgcgccgc
      961 tcgcggcgag cctcaaccag ctcgccagcg aactgaagct cccgccgctc gcttgagtga
     1021 ttaagtgact gagtgactaa gtgattgagt gccaccgcgc ctaggtctta atcacttaat
     1081 cacctaatca cctagtcact cgatgctacg agcgacccgc atgctcttct ggctgacgct
     1141 cattgccgcg accgcctggc tgctgtggta catggtcaag gtgcccggcg tctcatacac
     1201 gggcgcgctg aagccgctga ccagtgaaga gcagctcatc gccggcaacc tgcgccgcca
     1261 cgtcgcggcg atcgcgagcc gcgagcacaa cctgttccgg cccgcggagc tcgaggccgc
     1321 ggcgcagtat atcgagaaag ccctggccgg cgtggaccat gcggtcgcgg cgcagcgcat
     1381 tcaggcgggc cccatggaag tgcgcaatat tgaaatagaa gtaaccggtg aggggcgccg
     1441 cgatgaaatc atcgtggtcg gtgctcatta cgactcagta agcggcgccc ctggggccaa
     1501 cgacaacggc tcgggtgtcg gcgccatcat cgagctggcg cggctgttcc gggacgcgcg
     1561 gccggcgcgc acgctgcact tggtggcgtt cgtcaacgag gagccgccct tccaccacag
     1621 cgacgccatg ggcagccgcc agtactcgcg ccgatcgaag gagcgcggcg agaacatcgt
     1681 cgccatgttc tcgctcgaga ccatcggtta ttactccgac cgtccgggca gccagcacta
     1741 tccgttcccg ctcggcttct tctacccctc gactggaagc ttcatcgcct ttgtctcgaa
     1801 tctgtcctcc cgtgctctgc tgcacgaggt catcgcgagc ttccgccgcc acgccgaatt
     1861 cccctccgag ggcgtggccg cgcccgcctt catcccgggc gtggactggt ccgaccactg
     1921 gtcgttctgg caggagggct ggccggcgtt aatggtgacc gacaccgcgc cctatcgcta
     1981 tccgcactac cacacggcgc aggacacccc cgaccaggtg gactacgagc gcgccgcgcg
     2041 cgtggtgatc gggctctttc acatgctgcg cgcgctggcg cagtccgaat agccgccgac
     2101 cccacactgt tgcggcgcgg tgacagcgga agcgcctgaa acttcgagac ttgcgcgtgc
     2161 cgtggcccga tcgcgtcgtc cgacagcgac agtttcgcgc ccgaaaaact ccacgcgtac
     2221 gcgcaaagcc cttgatttgg ctggactaga gctgttggca tggcagttgc gccggcaggt
     2281 catgcgtcac ccaatgcggg agatgaagcc atgagaaccc ttaccaggat gctggcaatt
     2341 gcaggtatcg ccgcgatgac agcgggttgt gccagcgcgc cgcagacgcg gcaaggctgc
     2401 gcggaaccga actacggcgg cgcagtgata ggcggattgg ccggcggatt gctcggcgcg
     2461 caggtcggca aaggctcggg ccgcaccgcg gcggcggcgg tcggcgcggg cacgggcgcc
     2521 gtgatcggca gccaggtcgg ctgcgagtga tgtccctcga accgggagct caatcatgaa
     2581 aatattggcg catgttgcaa gcgggctggt tctcgtgact tcccttgccg cgcaggcggc
     2641 ggatacggcg cccgacgcgt tggtgaagag cacggtggac gaagtgctcg ccgtgatcaa
     2701 gcaaaccaag gaccgccaaa cgctggtgga actcgccgaa aagaaggttc tgccgcactt
     2761 cgatttcaag cacatgaccc agctcgcggt gggcaagtcg tggacgcagg ccaatgccgc
     2821 gcagcaggaa gcgctcgagc gtggattccg cgcactgctg atgcgcacct acaccgccgc
     2881 gctgtcacag agcagcggcg gggcgaaggt ggaggtgaag ccctcgcaaa tcaagccggg
     2941 cgacccggaa acggtggtgc gaaccctggt gaatgagccc ggtagaaagc ccttcagcat
     3001 cgattaccgc atgaggaacg ggtccggcgg ctggaaggtg tatgacgtgg tggtggaaaa
     3061 cctgagtctc gtcaccaact atcgtgcctc gttccaggcc gaagtcaacc gctcgggcat
     3121 agacgggttg atcaaggcca tcgaatccaa gaaccagcag caggggtagt cgctgtcagg
     3181 cgcgggggcg ggagcgcgcc ttttatcgcg gcacctgctt cgcataaaaa aaggccgccc
     3241 agcgtgaact aagtcacggc acttttgggc gcggtttcgg tctatgcggg aaggccaaaa
     3301 ttcgcccatg ccggtctatt actcgcgctc ctgtccgagg ctgaggccgc gtagctcgga
     3361 gaccatgcca tgaacgaacc gattcttccc ccgctggcta cgcggaagaa agtcctcctt
     3421 atcgacgacg accccgatct tctgtcgatc agaacgttgc ggttgcaggc ggacggttac
     3481 acggtgaaaa cggtgaacag cagcgcgacg gcgatggacg cgatcgcctc gtttcaaccg
     3541 gacctgatca ttttggacct catcatgccg gctatctccg gcgagaccct gctcaagcag
     3601 gtccgcggag atggatgctt cagagcaatc aagatcatag tcaacagcgc aaaaagtttt
     3661 gagtgtgacc agaagctctg ccttgaatcg ggcgcggatg cgtatctggc gaagcctgcc
     3721 gaacatgacg cgctggtcag ccttatcgga aaattgttgc gtgacgaggt gaccgttacg
     3781 ttctggggaa cgcggggcag cataccgaga cccggcaagg acaccttgag attcggcggc
     3841 aacacgccgt gtgtttcggt cgagctgggc gatgaccgcc tgttcatatt tgacgcgggc
     3901 accggcatcg tggacctggg acgggccctc gtcgccgcaa acaagcatta caaattcaac
     3961 cttttcgtgt cgcaccctca ctgggaccac atccaggggc tcccgttttt ccagcccttg
     4021 tacctgcagg ggaacgaaat ggtcatccat ggcaccagcc acggaaaact gagtctgcgg
     4081 gaagtcatca gcggacaaat ggacagcttg tattttccga tcaccatcaa ggagtacgcc
     4141 tcacgggtgt acttcaagga gctcgaggag ggcgactacg agattgagaa actgccgctc
     4201 agcactatca ccttgaacca ccccggccgg accctggggt atcggctggg taacggcaac
     4261 gggaagtccg tggcgtacat cacggataac gagatttttc cggaaggcga tcaatacaac
     4321 aggcgccggc ttgccgcttt cctgagcggc gtcgatgtcc tcatccacga tgcgacttat
     4381 tttgacgagg aatatccttc acgcgcaaga tgggggcatt cggcgctgtc ggaagttctc
     4441 aggctcgccg acgaagcgcg ggtgaaaagg ctgtacctgt ttcaccacga ccccagtcac
     4501 gacgacgacg ccgtggacaa gaaggaaacg ttcggcaggc gcttcttcga gaagcgcaat
     4561 tccgatgttc aatgttcggc cgcaagggaa ggagtgtcgg taacgctttg acggcggggg
     4621 tgctgccgca gctcttcaaa acaagggagg gttatgaaat cgggtgagcc aggtcacggc
     4681 gctttccggg agttgtcctg gtctacgggc tggggctaac cttcccccgt gcgacgcctg
     4741 tacccccgta cctttccccg cctgctggcg gtcgggttca cgctcgttgc gctgccgctg
     4801 gtgttcgcgc tcgtcaataa cgcgatctcg gtggaccggg tcgccaaccg cagccagaac
     4861 gcggtgtacc aggcggtgca ggccacccag aacagccggc ggctcgcgca actgctcacc
     4921 gagctcgagc gcaccgcgcg ccagatggtg atcctcgatg accgctcgct gcttgacacc
     4981 tatgccgcga accgccgtcg attcgagcag acggtggccg aattcgcccg cctgccgctc
     5041 gacgcggagc aaaagagcgc gctcgacgat gtcgtgcgcg gggagacgga gatcttttcg
     5101 gcattgtccg acgccggctc aaaaagggcg cagctgagga acgtcatcga gcgcttcgac
     5161 ggcctggccg agcaggctca ggccatcgtg gcgcgctcga acgcgcagat cgaccgcgag
     5221 gtcgaggcga tgcgcaccac cgccgaccag gctcaacgca tcacgttgtg gcaactgctg
     5281 gcgctggtcc cggtggtggt gttcctggtg atcggctttt ccatcctcat cacgcgcccg
     5341 atccgccagg tggacgccgc gatccgccgc ctcggcgccg gggagttccg cgtgccggtt
     5401 acggtcagcg ggccacagga cctcgaacag ctcggcgaac ggctggagtg gatgcggcgc
     5461 gagctggtcg atctggaaga gcagaagaac cgcttcctgc ggcaggtatc gcacgagctg
     5521 aaaacgcctc tcaccgcgct gcgcgagggc gccgagctgc tctccgagga ggcggtggga
     5581 aagctcaccc cggagcagcg cgagatcgcg gagattctgc gccacaacag tgtagaattg
     5641 cagaggctga tcgaagacct gctctccttc ggagcaagcc agttccgcaa ggtgacggtg
     5701 gatctggagc cggtggagat tcggcgcatc gtcgaacgcg tgaccaccga ccagcggctg
     5761 gcggcgaggg cccgcggcct gacgctcgac gtggcggccg aagacatcat ggtgcccgcg
     5821 gacccggaga aactgcgcgt cgtgctcgac aatctggtat ccaacgccat caggttctcc
     5881 cccgaggggg gctgcgtcag ggtcgtcgca tgcctcgaca ggggcgcgct caagctggac
     5941 gtgatcgatc aagggccggg catcccgccc gaggaacgca cgcgcatctt cgatcctttc
     6001 taccaggggc gccatgccgg tgcgggcccg gtacggggaa ccgggatcgg gctctccgtc
     6061 gtgaaggagt acgtattcgc ccacggcggc agcgtggaag t
//
LOCUS       DBNGWZ010000472         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2454986, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000472 DBNGWZ010000000
VERSION     DBNGWZ010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2454986"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..465
                     /locus_tag="AC16CX_14555"
     CDS             178..465
                     /locus_tag="AC16CX_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013763.1"
                     /translation="MSNPTIRPAPSAAKSNIPPVCPILTLGVLSPPSAIVGAPTKIAT
                     LPCLGPGCAFFHAAGACSVLVMADALLAQAHMMAANEGEETPTDAKPANPS"
     gene            479..>1698
                     /locus_tag="AC16CX_14560"
     CDS             479..>1698
                     /locus_tag="AC16CX_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013764.1"
                     /translation="MNNEREQQIAILAQRAQQDGDDWRVKIARGDSPGGRIEQLATFD
                     GATVEQIANADTWIPPLMGGTGNVGVYYLYVQHAKDRAVGRTSALLQLPGISGAPTPS
                     AQIPWTRIEAPGWIGPRILVYPNPADLGTRPGAPVSASPVPPTGAGAGSPFFSSVPPG
                     TPEALAAELRAEQRKLDEARQRAELEAIKRANEEAQRRSDAHMAEMREMVKSLVAIAQ
                     RPQTDLVELIAKGAAVLAPILTPLLAGRGATEQARVAAEERRWEREEARRIEERKREE
                     DRAAESAKERNAMMERIANQGAEQSKAQAALSEAHATSMRTMIQTVTSLMELGIGRPA
                     QEENPWAEMGKAFAAAFGERMAMGSGNMTPPALPPRSSGPLGTPPTTQPAAASTQAAP
                     AAPSQPASDGNAVSL"
BASE COUNT          303 a          611 c          546 g          238 t
ORIGIN      
        1 atcgatcacc cttccctcat ggagaaatga ccatgaaagg cacggtactc gtgaagccgt
       61 gcgcctgtcg aagggtgtta cttcatcggc cgcgcagcgg gcaaacatgg cgccccgtca
      121 tcgcgcacgg cttcaagcac ggccgatcct actgcgttgc actcaaggag aaatgacatg
      181 tcgaacccca caattcgtcc cgctccgtct gcggcgaagt caaacattcc ccccgtctgc
      241 ccgattctca cgctcggcgt tctctccccg ccctccgcga tcgtgggcgc gcccacgaag
      301 atcgcgactc ttccctgcct cgggcccggg tgcgcgttct tccacgcggc cggcgcgtgc
      361 tccgtgctcg tcatggccga cgcgctcctc gcgcaggcgc acatgatggc ggccaacgaa
      421 ggcgaagaga cgccgaccga cgccaagccg gcgaacccga gctagaaagg ctccccccat
      481 gaacaacgaa cgagagcagc agatcgcgat cctcgcgcag cgcgcacagc aggacggtga
      541 cgactggcgc gttaagatcg cgcgcggcga ttcccccggc ggccggatcg aacagctcgc
      601 gaccttcgat ggtgccaccg tcgagcagat cgcgaacgcc gacacctgga tcccgccact
      661 catgggcggc accggcaacg tcggtgtcta ttacctttac gtccagcacg cgaaggatcg
      721 cgcggtcggt cgtacctccg cgcttctcca gctccccggc atctcggggg caccgacccc
      781 ctccgcacag atcccatgga cgcggatcga ggcaccgggc tggatcgggc cgcgcatcct
      841 cgtctatccc aaccccgccg acttggggac ccgccccggc gctccggtga gcgcctcgcc
      901 cgtgccaccc actggcgccg gggcagggtc cccttttttc tcgtcggtgc cgccgggcac
      961 ccccgaagca ctcgccgccg agctgcgcgc cgagcaacgc aagctcgacg aggctcgaca
     1021 acgcgccgag ctggaagcga tcaaacgcgc gaatgaagaa gcgcaacggc gctcggatgc
     1081 tcacatggcc gagatgcgcg agatggtgaa gagcctcgtt gccatcgcgc agcgtcccca
     1141 gacggatctc gtcgagctga tcgcgaaggg cgcagccgtg ctcgccccga tccttacgcc
     1201 gctcctggcg ggacgtggcg ccactgaaca ggcgcgcgtt gctgccgagg agcgtcgatg
     1261 ggaacgcgag gaggcgcgcc gcatcgagga acgcaagcgc gaggaggatc gcgcggccga
     1321 gtccgcgaag gagcgcaacg cgatgatgga gcgtatcgcg aaccagggcg cggagcagtc
     1381 gaaggcgcag gccgcgctct ccgaggcgca cgccacgtcc atgcggacga tgatccagac
     1441 cgtcacatcg ctgatggagc tgggcatcgg gcgtccggcg caagaagaga acccgtgggc
     1501 cgagatgggc aaggcgttcg ccgccgcctt cggagagcgc atggcgatgg gctcggggaa
     1561 tatgactccg cccgcgctgc ctccgaggag tagcggtccg ctcggaacgc ctcccacgac
     1621 gcagccggcc gcggcttcga ctcaggccgc ccccgctgct ccgtcacagc ccgcttcgga
     1681 cggcaacgcc gtgagcct
//
LOCUS       DBNGWZ010000473         9691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2204090, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000473 DBNGWZ010000000
VERSION     DBNGWZ010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2204090"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16CX_14565"
     CDS             <1..218
                     /locus_tag="AC16CX_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3013765.1"
                     /translation="QVFAEENALDKLEAFASLNGPRHYGLPPNREAITLERAPWTAPL
                     EVKVNGPEERALVHRGGETIAWRVTSA"
     gene            231..818
                     /locus_tag="AC16CX_14570"
     CDS             231..818
                     /locus_tag="AC16CX_14570"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3013766.1"
                     /translation="MEFDNTLEVPLPPDEAWKVLLDIRRIATCIPGAELTEVVDERTY
                     KGQVAVRLGPVALSLVGQARLEEIDHANRKARVKAQGSDPKGRGSTDSVIEFRLEPAG
                     NGTRILIHSDVRLAGSIAQYGRGSGMIQSVASQLIGQFGEALKAQIAQSRPSTAATAE
                     PAPAPPAKPISGLSLIARALWDSIARLFRKPPPKR"
     gene            823..1680
                     /locus_tag="AC16CX_14575"
     CDS             823..1680
                     /locus_tag="AC16CX_14575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3013767.1"
                     /translation="MKPAPFEYHAPKTIDEAVALLAEVASRDGRVLAGGQSLVPAMAL
                     RLALPAHLVDINGVTGLDRVAVEENELSIGACVRHAVFHRPVADGPLGELLSHVARHI
                     AHYPIRTRGTFCGSLAHADPASEWCLVAATLGAKMVARSTRGTRILQAADYFQGPMTT
                     ALALDELLLEVRLPLLPPDTRFGFCEFSRRAGDFAIAMALAAYRLEEGVIAEPRIGVG
                     GVEGRPRRIAEAEAALEGKHPSSNVFRAAADTAARAVEPLDDIKYPAGYRRDLARAMT
                     RRALESAAA"
     gene            1677..2153
                     /locus_tag="AC16CX_14580"
     CDS             1677..2153
                     /locus_tag="AC16CX_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3013768.1"
                     /translation="MKVGVTLNINGKNHAFQVEPRRTLADAIREECGQTGTHIGCEHG
                     VCGACTILLDDEPVRSCLMFAVQAAGRKIRTVEGLADGEKLHPLQRAFVEHHGLQCGY
                     CTPGFLMLAAGTLERNPDIGDAELLEILSSNLCRCTGYKNIIKAVRAAAAEMKPRR"
     gene            2150..4456
                     /locus_tag="AC16CX_14585"
     CDS             2150..4456
                     /locus_tag="AC16CX_14585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3013769.1"
                     /translation="MKTKFVGQPVLRLEDPPLVTGRGRYAADINFPGQLHMRVVRSSH
                     AHGRIRSIDVERARAAPGVVAVWTGADVAGLKPIALREGPDEKLDPYLQPVLARHQVR
                     YVGEPVAAVFAEDAYLAEDAADLVSMEMEERPVVLDAHEPPSEFESGRSTEPLVIRKG
                     YGDIEAAFRAAHAIVELDLSIGRHSGVPLETRGAVARYDAGRDLIQMYGAAKVPHRTR
                     DGIARVLGRDPAKIHLYEGHVGGGFGVRGEVYPEDVLVCLGAMRLGRPVKWIEDRYEN
                     LIATNHSRQQRHRVRAAVDREGRILALDDEFYLDQGAYPRTHGARVADLTAGQLPGPY
                     HVPAYRVVAHYRLTNKTPAATYRSPGRFEGSFVRERLMDAIAQRLGLDSIEVRHRNLI
                     PKTAMPYRRDLDTIGDQVVLDSGDYSGLLDKALAGIGWDALQKDLKRRRAAGEAVGAG
                     VAMYLEKSGLGPRDGARISVDGSGGVEVVTGSASLGQGVETVMAQICADALGVDYRRV
                     RVIHGQTDRIAEGIGAHASRATVMTGSAVHVAASNVRAKAIEAAAKLMRVPADALDIV
                     NGKVVRKDSPDAISMDLGEVARSLASGRDASGLSAEGVFETEHMNYPYGVHVAVVKVD
                     RETGGVDVERYLIAYDVGRAVNPMLVEGQMAGGFAQGLGGALLEEFQYDERGQPLSVT
                     FADYLQPTVHETPMVEIIVSEDAPSPLNPLGIKGAGEGGVTAVGAAIAAAIDDAIGVP
                     GAVTRLPVTPVRLKEILDRADNRHGAAS"
     gene            4453..5772
                     /locus_tag="AC16CX_14590"
     CDS             4453..5772
                     /locus_tag="AC16CX_14590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013770.1"
                     /translation="MSTVSGISALAPFRVRSFRFQWPADLATSWAFEMESVILGWYVL
                     VESRSVFMLTVFASLLYVGTLLAPISGVIGDRIGNRNLLGAMRGLYTVLSTTLMTLAF
                     LDAVTPALVLAIATVLGLVRPSDIGVRAALVGETMPAERLMGAISIQRTTLDSARIVG
                     ALTGAGLVATLGMGPAYLVVASFYAISVLLTLQIHSTRSTHRAAGDEAGGFKRPRDTL
                     AEYQASEAGAKARPSPWRDLKEGLGYVWSTPHLLAIMCLAFLVNLTAFPLFLSLMPYV
                     AKEIYQADQTMLGYMVASAAFGALAGSIALSRHGGAIRPARMMLVFCAVWYATLLAFA
                     HTQHPSAGIPVLVLAGCAQSLGLVPMAVLLLRTSDARFHGRIMGIRMLAIYGNLPGLL
                     LSGPLIARFGYPFTATLYCAIGLAFTLLVAVRWRAHLWRSNAPANAR"
     gene            complement(5791..6756)
                     /locus_tag="AC16CX_14595"
     CDS             complement(5791..6756)
                     /locus_tag="AC16CX_14595"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013771.1"
                     /translation="MNTITRALACVALASAAALAHGQGYPTKSIRLIVPYAPGGIVDY
                     VGRLIGQRLTEGFGQSVVVDNRPGAGGIIGIETASRANGDGYTLVIMDPAIVINPSLL
                     PKVPYDIHKDLVPVTILSNSPLVLAINAKVPAASVQELVNLAKSQPGKLSFASAGVGT
                     TPHMAGELFKARIQQNIVHVPYKGSGPAMTDLIGGQVQMTFSSITAALPFIKDGRLRG
                     LATTGAKRAAALSGTPTMVESGFPGFEVNLWLGLFVQSSTPKSIVAALNAETRKALQH
                     PAVAGGFEKVGAEPLGNSSQDAQAYVKSEFTKWAKVIRDGKLRAD"
     gene            6907..9273
                     /locus_tag="AC16CX_14600"
     CDS             6907..9273
                     /locus_tag="AC16CX_14600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3013772.1"
                     /translation="MNDFADIRAAYTAIGAPVERVEDERLLRGQGKYVDDVRRPDLLH
                     AAVLRSPLAHGRIRSIDASAALAMPGVHAVITAADVVFELGAVPVIPQRQEALAELEP
                     FYQPVIVQSKVRYAGEPITVVVARDRALAEDALARIKVDIEALPVVGDCAAAARNESL
                     LFEEAARNCAVTLTASKGDADAVFASAPYTRRERFRVHRHMAAPMETRGLLADWDAAA
                     GKLVVYGAAKTAFRNRKVLAGQMRMNEADIELVEGDVGGGFGARGEFYPEDFLIPFAA
                     RRIRRAVKWIEDRREHFIATNHAREAYCELEIACTTDGIILGLRGRAQTDAGAYVRTN
                     GVTPGRNLAQMCTGPYRVPHVRVEVSLMLTNKTPVGTYRAPGRFEADYFRERLLDIAA
                     HDLAIDRVEFRRRNFIAPHEMPYPLATVMPLNGKTATDSGDYRATLERCLAEFDWAGK
                     ARLNGKLIDGRYHGIGIGCYIEGGASGPAEGARLVVETDGTVSVYTGSSSLGQGLETV
                     FTQIAADALGMPMSRIRGVFHGSTSGVSEGWGAYSSRSVVMGGSAIVETAKRLLERIR
                     ELAAARLGCAPAEVEIRDGCHSLAWAGMSVTLGELAAASAQSGGISEEGKFLSNKRTY
                     SYGTHAAHVAVDPDTGRVEVIDYVAVEDVGRIINPHTLHGQAVGAIVQGLGGVLSEHL
                     VYNEDGQLQTGTLADYLMPTAGEFPRIKVIALEQYPCPNNPLGAKGAGEGGIIPVGGT
                     ICNAVAAALAPLGVEPRELPLSPPRVWKMIEDARARTAPESMEPQAIR"
     gene            9270..>9691
                     /locus_tag="AC16CX_14605"
     CDS             9270..>9691
                     /locus_tag="AC16CX_14605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3013773.1"
                     /translation="MKPPPFLYHDPETVADAIGLLGRLENAKLLAGGQSLMPMLSMRY
                     VLPDHVIDLNRVKGLSYLRETGDALEIGAMTRQRDIEFSDLVRARCPLMHEAILQVGH
                     RQTRNRGTLGGSLCQLDPSAELVAVAAALDATVSVTGP"
BASE COUNT         1542 a         3201 c         3335 g         1613 t
ORIGIN      
        1 ctcaggtgtt cgcggaggag aacgcgctcg acaagctcga agcgtttgcc tcgctcaatg
       61 gaccgcggca ctatggattg ccgccaaacc gggaggcgat cacgctcgag cgcgcgccgt
      121 ggaccgcgcc gttggaagtg aaggtcaatg gccccgaaga acgggcgctg gtccaccgcg
      181 gcggcgagac gatcgcgtgg cgggtgacct cggcctgacc gggttagagc gtggagttcg
      241 acaacacgtt ggaagtaccg ctgccgccgg acgaggcgtg gaaagtgctg ctcgacatca
      301 ggcgcatcgc cacctgcatt ccgggcgcgg aattgacgga agtggtggac gagcgcacct
      361 acaagggcca ggttgccgtg cgcctcggcc cggtcgcgct gtctctggtc gggcaggccc
      421 ggctcgaaga gatcgatcac gcgaaccgca aggcccgggt caaggcgcag ggatcggatc
      481 ccaagggacg cggcagtacg gattcggtga tcgagttccg tcttgagccg gccggcaacg
      541 ggacccggat attgatccat tccgacgtga ggctggcggg ctcgatcgcc cagtacggcc
      601 ggggctccgg catgatccag agcgtcgcct cgcaactgat cgggcagttc ggggaagcgc
      661 tgaaagcgca gatcgcgcaa tcacgacctt caacggcggc caccgcggaa ccagcgccgg
      721 cgcccccggc gaagccgatc tcggggctgt cgctgatcgc gagggctctg tgggactcga
      781 tcgcgcggct gttcaggaaa ccgccgccga agcggtaaag agatgaagcc cgcgcccttc
      841 gaatatcatg cgccgaagac aatcgacgag gcggtggccc tgctcgccga agttgcgtcg
      901 cgggacgggc gggtcctggc cggcggccag agcctcgtgc cggcgatggc gttgcgactc
      961 gcgctacccg cgcatctcgt ggacatcaac ggcgtaaccg gtctcgatcg cgtggcggtg
     1021 gaagagaacg aactttcgat tggcgcctgc gtgcggcacg ctgtcttcca tcggccggtt
     1081 gccgacgggc cgttgggaga gctgctctca cacgtagccc gccacatcgc gcactatccg
     1141 atccggaccc gcggcacctt ctgcggcagc ttggcccacg ccgaccccgc atcggagtgg
     1201 tgtctcgttg ccgcgacgct cggcgcgaag atggtggcca ggagcacgcg cggcacgcgc
     1261 atcctccagg ctgccgatta tttccaggga ccgatgacga cggcgcttgc cttggacgag
     1321 ctgctcttag aagtgcgcct gccgttgctt cctccggata cgcggttcgg tttttgtgag
     1381 ttcagccgcc gagccggcga ttttgcgatc gccatggcgc tcgcggccta ccggctcgag
     1441 gaaggcgtca ttgccgaacc gcgcatcggc gtcggcggcg tcgagggccg gccgagacgg
     1501 atcgccgaag ccgaagccgc actggaagga aagcacccct ccagcaatgt attccgggcg
     1561 gctgcggaca ctgcggcccg tgccgttgag ccactcgacg acatcaagta tcccgccggc
     1621 tatcggcgcg atctcgcgcg cgcgatgacg cgccgggcac tggagagcgc tgcggcatga
     1681 aagtgggcgt cacgctcaac atcaacggca agaaccacgc attccaggtg gagccgcggc
     1741 gcacgctcgc cgacgcgatc cgcgaggaat gcggccagac cggtacccat atcggctgcg
     1801 agcacggcgt gtgcggcgcc tgcaccatcc ttctcgacga cgagccggtg cgttcgtgtc
     1861 tcatgtttgc cgtgcaggcg gcggggcgga agatccgcac ggtcgaaggg ctggccgacg
     1921 gcgaaaagct gcacccgctt cagcgcgcct tcgtcgagca ccacggcctg caatgcggct
     1981 actgcacccc gggctttctg atgctcgccg ccggcacgct ggagcgcaat cccgatatcg
     2041 gtgacgcgga gctgctcgaa atcctgtcct cgaacctgtg ccgctgcacc ggctacaaga
     2101 acatcatcaa ggccgtgcgc gcggcggcgg ctgaaatgaa gccgcgccga tgaagacgaa
     2161 gttcgttggc cagccggtcc tgcgcctgga ggatcccccg ctcgtcaccg gccgcggccg
     2221 ctacgccgcg gacatcaatt ttcccggcca gcttcacatg cgcgtcgtgc gctccagcca
     2281 cgcgcacggc aggatccggt ccatcgacgt ggagcgggca cgcgcggcgc cgggtgtggt
     2341 cgcggtgtgg accggggctg acgttgcggg tctgaagccc attgcgctcc gcgaggggcc
     2401 cgatgaaaag ctcgacccct atctccagcc ggtactggcg cggcaccagg tgcgttacgt
     2461 aggtgagccg gtcgcggccg tgttcgcgga ggacgcgtac ctcgcagagg acgcggcaga
     2521 cctcgtcagc atggagatgg aggagcggcc cgttgtgctg gacgcgcacg agccgccctc
     2581 cgaattcgag tccgggcgct cgaccgagcc gctggtgata cgcaagggct acggcgacat
     2641 cgaggcggcc ttccgcgccg cgcacgcaat cgtcgagctc gacctctcca tcggccgcca
     2701 ttcgggcgtg ccgctggaga cgcgcggcgc cgttgcgcgc tacgacgcgg ggcgcgacct
     2761 cattcaaatg tacggcgcgg ccaaggtgcc gcaccgcacc cgcgacggga ttgcgcgcgt
     2821 gctcgggcgc gatccggcga aaatccattt gtacgagggg cacgtgggcg gcggcttcgg
     2881 agtgcgcggc gaggtctatc ccgaggacgt gctggtctgc ctgggcgcga tgaggctcgg
     2941 gcgcccggtg aagtggatcg aggatcgtta cgaaaacctg atcgcgacca atcattcccg
     3001 tcagcagcgg caccgggtgc gcgcagcggt ggaccgcgaa ggacgcatcc tggccctgga
     3061 cgacgagttc tacctcgacc agggcgctta cccgcgtact cacggcgcga gggtggcgga
     3121 cctgacggcc ggccagttgc cggggcccta tcacgtaccg gcttaccgcg tcgttgcgca
     3181 ttaccgtctc accaacaaga ccccggcggc gacctatcgc tcgccgggcc gcttcgaagg
     3241 cagtttcgtg cgggagcggc tgatggatgc catcgcacag cggctcggcc tcgattcgat
     3301 cgaagttaga caccgcaacc tgatcccgaa aacagcgatg ccgtaccggc gcgacctgga
     3361 caccattgga gaccaagtcg tgcttgactc gggcgactac agcgggctgc tcgacaaggc
     3421 cctggccggc atcggctggg atgcgctgca gaaggacttg aagcgccgcc gcgcggcggg
     3481 cgaggcggtc ggcgcgggcg tggccatgta cctggagaag agcggattgg gtccgcgcga
     3541 tggcgctcga atctcggtgg atggctctgg cggcgtcgag gtcgtcaccg gttcagcttc
     3601 gctggggcag ggcgtggaga cggtgatggc gcagatctgc gccgatgcgc tgggcgtgga
     3661 ctaccggcgg gtgcgcgtga tccacggcca gaccgaccgc atcgccgagg gcatcggcgc
     3721 gcacgcctcg cgcgcgacgg tcatgaccgg ctcagcggtc cacgtcgcag cctcaaacgt
     3781 gcgcgccaag gcgatcgaag ccgccgccaa gctgatgcgg gttcccgccg acgcgctgga
     3841 tatcgtcaac gggaaggtcg tgagaaagga cagtcccgac gcgatctcga tggaccttgg
     3901 cgaggtcgcg cggtcgctgg catcgggccg ggatgcatcc ggtctctcgg ccgagggcgt
     3961 gttcgagacc gagcacatga attatcccta tggcgtccac gtcgccgtgg tgaaggtgga
     4021 ccgggaaacc ggtggggtgg acgtggagcg ctatctcatc gcctacgacg tcgggcgcgc
     4081 ggtcaacccg atgctggtcg agggccagat ggccggcggc ttcgcgcagg ggctgggcgg
     4141 cgcgctgctg gaggagttcc agtatgacga gcgcggccag ccgctgtcgg tcaccttcgc
     4201 tgattacctg cagcccaccg tgcacgagac gccgatggtg gagatcatcg tctcggaaga
     4261 cgcgccgagc ccgctcaatc cgctcggcat caagggggcg ggcgagggcg gcgtcaccgc
     4321 ggtgggcgcg gcaatcgccg cggcgatcga cgatgcgatc ggcgttccgg gcgccgtgac
     4381 gcgcctgccc gtcacgccgg tgcggctcaa ggaaatcctg gaccgcgcag acaaccgcca
     4441 cggtgccgcg tcttgagcac ggtttcggga atctcggcgc tcgcgccgtt ccgggtccgc
     4501 agcttccggt tccagtggcc cgccgacctg gcgacatcgt gggcgttcga gatggagtcg
     4561 gtgattctcg gctggtacgt gctggtcgag agccggtcgg tgttcatgct caccgtgttc
     4621 gcgtcgctgc tgtacgtcgg caccctgctc gcgccgatat ccggcgtgat cggggaccgc
     4681 atcggcaacc gcaacctgct cggcgccatg cggggcctct acacggtgct ctcgacgacc
     4741 ttgatgaccc tcgctttcct ggacgccgtc acgccggcgc tcgtgctggc catcgccacg
     4801 gtgctgggcc tggtgcgtcc ttcggacatc ggggtgcgcg ccgcactggt cggcgaaacc
     4861 atgccggccg aacgcctgat gggggcgata agcatccagc gcactaccct ggattccgcg
     4921 cggatcgtgg gcgctttgac cggagcggga ctggtcgcga cgctcggcat gggaccggcc
     4981 tacctggtgg tcgcgagctt ctatgcgatc agcgtactgc tgaccctgca gattcacagc
     5041 acgcgctcca cgcaccgtgc agctggagat gaggcgggcg ggttcaagcg cccccgcgac
     5101 acgttggcgg aatatcaggc ttcggaagcg ggggccaagg cgcgcccctc gccctggcgc
     5161 gatctcaagg aagggctcgg ctacgtctgg agtacgccgc atctccttgc aattatgtgt
     5221 ctcgcctttc tggtcaacct gacggcgttc ccgctgttcc tcagcctgat gccgtacgtg
     5281 gcgaaggaga tctaccaggc cgaccagacg atgctcggct acatggtcgc gagcgcggct
     5341 ttcggcgccc tggcgggttc gatcgcgctg agccgccatg gcggcgccat ccgccccgcg
     5401 cgcatgatgc tcgtcttctg cgcggtgtgg tacgcgacgc tcctcgcgtt tgcgcacacg
     5461 cagcatccct cggccggcat cccggtcctt gtcctcgccg gctgcgcgca aagccttggc
     5521 ctggttccga tggcggtgct gctactgcgc acctcggacg cgcggttcca cggccgcatc
     5581 atggggatac ggatgctggc gatctacggc aacctgcccg ggctgctgct ctccggtccc
     5641 ttgatcgcgc gcttcggcta cccgttcacc gccacgcttt attgcgcgat cggcctggcg
     5701 ttcaccctgc tggttgccgt gcgctggcgt gcgcacctct ggcggagcaa tgctccggcg
     5761 aatgcacggt gaaatctcgg tggttcgctc tcaatcggcg cggagcttgc cgtccctgat
     5821 gactttcgcc cacttcgtga attcgctctt cacgtaagct tgagcgtcct gggacgaatt
     5881 acccaggggt tcggcgccga ctttctcgaa tccacccgcc accgcggggt gttgcagcgc
     5941 tttccgggtt tccgcattga gcgctgcgac gatggacttc ggcgtgctcg attgcacgaa
     6001 caggccgagc cagagattga cctcgaatcc cggaaaaccg gattccacca tggttggcgt
     6061 gccggatagg gcggcggcgc gtttcgcgcc ggtcgtcgcc aggccgcgca gccggccgtc
     6121 cttgatgaag ggcagggcgg cggtgatgct gctgaaggtc atctgcacct gcccgccgat
     6181 gagatccgtc atcgcgggcc cgctcccctt gtacggcacg tgtacgatgt tctgctggat
     6241 cctcgccttg aacagctcgc ccgccatgtg cggcgtcgtg ccgacgccgg ccgaggcaaa
     6301 gctcagtttc ccgggctgcg atttcgcgag attgaccagc tcctgcacgc tcgcggccgg
     6361 caccttggcg ttgatcgcca acaccagcgg cgagtttgac aggatggtga ccggcacgag
     6421 gtccttgtgg atgtcgtacg gcaccttcgg cagcaggctg ggattgatca cgatcgccgg
     6481 gtccatgatc acgagcgtgt aaccgtcgcc gtttgcgcgg ctcgcggtct cgatgccgat
     6541 gatgccgccg gcgccgggtc ggttgtcgac caccacgctt tgcccgaatc cctcggtgag
     6601 ccgctggccg atcagccgtc cgacgtaatc gacgatgccg cccggcgcgt agggcacgat
     6661 caggcggatg cttttcgtgg gatagccctg gccgtgcgcg agagcggccg cgctcgccag
     6721 cgccacgcag gcgagcgcgc gagtaatcgt attcatgcgg aaactccggg gatgaatgtt
     6781 cttgatcggg acgcgggcgg ctttcaccgc ggtccgatcc tgctactatt cggctaactt
     6841 tacctgatgg tcacgactcc ttcaattcga tttttaggac gcggcgggcg ctagaatgcc
     6901 acggacatga atgacttcgc ggacatccga gccgcataca cggcgatcgg cgcgcccgtc
     6961 gaacgcgtcg aggacgagcg gctgctgcgc gggcagggaa agtatgtcga tgacgtgagg
     7021 cgcccggact tgctacacgc ggcggtcctg cgcagcccgc tcgcgcacgg ccgcatccga
     7081 tcgatcgacg cttccgccgc actcgccatg cccggcgtcc acgccgtcat caccgcggcg
     7141 gacgtcgtat tcgagctggg cgcggtgccc gtcattccgc agcgccaaga ggcgctcgcc
     7201 gagctcgagc ccttttatca gccggtcatc gtgcagtcca aggtgcgcta tgccggcgag
     7261 ccgatcacgg tcgtcgtcgc gcgcgaccgc gcgctggcgg aagacgccct ggcccgcatc
     7321 aaggtcgata tcgaagcgct gcccgtggtc ggtgactgcg ccgccgccgc gaggaacgag
     7381 tccctgctgt tcgaggaggc ggcgcggaac tgcgcggtca cactcacggc cagcaagggc
     7441 gatgccgatg cggttttcgc atcggcgccc tacacccggc gcgagcgctt tcgcgtgcat
     7501 cgccacatgg cggctccgat ggagacgcgc ggactgcttg ccgattggga cgctgcggct
     7561 ggcaagctcg tggtttacgg agcggccaag acggcgttcc ggaatcgcaa ggttctcgcc
     7621 ggccagatgc ggatgaatga ggccgacatt gagctggtgg aaggagatgt gggaggagga
     7681 ttcggagcgc gcggcgagtt ttacccggaa gacttcctga tcccgttcgc cgcgcggcgg
     7741 atccggcgcg ccgtgaaatg gatcgaggac cggcgcgagc acttcatcgc caccaatcac
     7801 gcgcgcgagg cgtactgcga gctggaaatc gcgtgcacca cggacggcat catcctcggg
     7861 ctgcgcggcc gcgcgcaaac cgacgccggc gcctacgtgc gcaccaacgg cgtcacgccg
     7921 ggacgcaacc tggcgcagat gtgcaccggt ccctatcgcg taccgcacgt gcgggtggaa
     7981 gtgtcgctga tgctgaccaa caagacgccc gtcggcacct accgcgcgcc cggacgcttc
     8041 gaggccgact acttccgcga gcggctgctc gacatcgcgg cccacgatct cgccatcgat
     8101 cgcgtcgagt tccgccgccg caatttcatc gccccccacg agatgccgta tccgctcgcc
     8161 accgtgatgc ccttgaacgg caagactgcg accgacagtg gcgattaccg cgccacgctc
     8221 gagcgctgcc tggcggaatt tgactgggcc ggcaaggcgc ggctcaacgg caaattgatc
     8281 gatggccgtt accacggcat cggcatcggc tgctacatcg agggcggcgc ctcgggtccg
     8341 gcggaagggg cgcggctcgt cgtcgaaacc gacggcacgg tttcggtcta caccgggtca
     8401 tcttcccttg gccaggggct cgaaacggtg tttacccaga tcgccgccga cgcgctcggc
     8461 atgccgatgt cgcgcatccg cggcgtgttc cacggctcga cctccggcgt gagcgagggc
     8521 tggggcgcct acagctcgcg cagcgtggtc atgggcggct ccgcgatcgt cgagacggca
     8581 aagcggctgc tcgagcgcat tcgcgagctc gccgccgccc gcctgggctg cgcgccggca
     8641 gaagtcgaaa tccgcgacgg atgccactcg ctcgcgtggg ctggcatgtc ggtcacgctc
     8701 ggcgagctgg cggcggcgag cgcgcaatcg ggcggcatct ccgaggaagg caagttcctg
     8761 agcaacaagc gcacctacag ctacggtacg cacgccgccc acgtcgccgt ggatccggac
     8821 accggccgcg tggaagtgat cgactacgta gcggtggagg acgtcggccg catcatcaat
     8881 ccgcatacct tgcacggtca ggctgtcggc gccatcgtgc aggggctggg cggcgtgctc
     8941 tccgagcacc tcgtctacaa cgaggacggc cagcttcaga ccgggaccct tgccgattac
     9001 ctgatgccga ccgcgggcga gttcccgagg atcaaggtga tcgcgctgga gcagtacccg
     9061 tgcccgaaca atcctctggg cgcgaagggc gccggcgaag gcgggatcat tccggtgggc
     9121 ggcaccatct gcaatgcggt cgcggcagcg ctcgcgccgc tcggtgtcga gccgcgcgag
     9181 ctgccgttat caccgccgcg ggtctggaag atgatcgagg acgcccgcgc gcgcaccgct
     9241 ccggaatcga tggagccgca agcgattcga tgaaaccgcc gccattcctc tatcacgatc
     9301 cggagacggt cgccgacgcc atcgggctgc tcggccgtct cgagaacgcc aagctgctcg
     9361 cgggcggaca atcgctgatg ccgatgctca gcatgcgcta cgtgctgccc gatcatgtga
     9421 tcgatctcaa ccgggtcaaa gggctttctt atctgcgcga gaccggagat gcgctggaga
     9481 tcggcgcgat gacccgccag cgcgacatcg agttttccga tctcgtccgt gcgcgctgcc
     9541 cgttgatgca cgaggcgata ctgcaggtcg gccaccgcca gacgcgcaat cgcggcacgc
     9601 tcggcggctc gctgtgccag ctcgatccct cggcggagct ggtcgcggtc gccgccgcgc
     9661 tcgacgcgac ggtcagcgtc accggcccga a
//
LOCUS       DBNGWZ010000474         5146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2961171, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000474 DBNGWZ010000000
VERSION     DBNGWZ010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2961171"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..347
                     /locus_tag="AC16CX_14610"
     CDS             165..347
                     /locus_tag="AC16CX_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013774.1"
                     /translation="MADVTRPPNPSEMDELEEGIPVMQQLLDNPFLLLFLGIAMPTVL
                     YIIWGVMEIVGIPVAK"
     gene            387..926
                     /locus_tag="AC16CX_14615"
     CDS             387..926
                     /locus_tag="AC16CX_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR3013775.1"
                     /translation="MSAIQPPGARVWWKQPLDRIELVWILLALVWCLVMFFMMPYWHV
                     FGKQNLANEAYRTTPEAYMKKATDVVEKYTVRKETERQIPVIHPPAGSDVYLVARLWE
                     WWPLLELEAGKSYRLHLMSMDWLHGFSLQPENINIQVHPGYDHVLTITPTSPGTYSIV
                     CNEYCGVNHHTMSSRLYVK"
     gene            939..2660
                     /locus_tag="AC16CX_14620"
     CDS             939..2660
                     /locus_tag="AC16CX_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR3013776.1"
                     /translation="MALNRMFRTCPTTGLQFHEPSEALIKANAVAGVVSLLVGGVLAL
                     LVVLTRWPAVHLLPADWFYLVLTGHGLDMLVFWIIFFEIAILYFCSSTLLRCRLATPK
                     MAWAGFALMVIGALMNNFAVFKGDSSVMFTSYAPMGAHPTFYLGLILFAVGALIGCFV
                     FLGTLVVGKAEGTYNGSIPLVTFGALTACIIAVFTIAAGAIILIPTFLWSVGLIGNID
                     SLMYRTVWWAFGHSSQQVNVSAHVSVWYAVAAIVFGAKPLSEKVSRTAFFLYILFLQL
                     ASAHHLLADPGLTSNWKIFNTSYAMYLAVLASMIHGFTVPGAIEVAQREKGYTKGLFE
                     WLRKAPWGNPVFSGMFMSLLGFGFLGGISGVVLGTEQLNLIMHNTFYVPGHFHATVVV
                     GTTLAFMAITYFLIPVLFRREVMFPKLAQWQPYLFGIGMSAFALFMMGAGTLGVPRRH
                     WDMTMAGNLMPHEWPGTAYLMVGLMAISGLVAIVGGVAYLLITVGSIFFGKRIETPGY
                     RHMRVAEGRAAPAPAQPVAAAITGHGGTGIAVAPGTFVLAMVLLVSFVLYYFVNWKYL
                     SQVWPLS"
     gene            2725..3588
                     /gene="cyoE"
                     /locus_tag="AC16CX_14625"
     CDS             2725..3588
                     /gene="cyoE"
                     /locus_tag="AC16CX_14625"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR3013777.1"
                     /translation="MAAMRLWFGLFKLRIGTLIAITALAGAAVTPGPPLTAWQYVVLA
                     LSVLAASAAAGAFNQFYERDLDLRMARTRRRPFVTGALQPHAAWLALIALLLAAATVF
                     AATVLNAASAAYVFLGAFVYGVVYTVWLKRRSWLNIVIGGLAGSFAVLAGAAAVTPAL
                     DATPIILAVVLFLWTPPHFWCLAIAFHRDYAAAGVPMLPVVIGDAGAARVIFHNTVML
                     VATSLLPLAFGMGALYAAGAVLGGALFLGRGYVLMQEPTPAAAMRCFHASLAQLALLL
                     VGAMLDVALRS"
     gene            3605..4516
                     /locus_tag="AC16CX_14630"
     CDS             3605..4516
                     /locus_tag="AC16CX_14630"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR3013778.1"
                     /translation="MWYAVLQPEMLSALLGISASAGAALPPSAPRDAAVPVRAEALAA
                     PFPRPMPLEKARATAGLDMDQALRVSQAAMGREIGDYAFFDREGRRVRLSDYRGKPLV
                     VNFVYTGCSQVCPTATRFLARAIQNAQQALGPDKFNAVTIGFNVPFDTPLAMKNFAAR
                     HGVSLPNWEFLSPDRETVAALTADFGFSYALTAGGFGHITQLTLVDPRGRIVRQIYGE
                     GFELPILVEPLKQLVAGRPTLADGAAQALDKVRILCTIYDPALGRYRLNYGLFIAILV
                     GLSVLGSTGYLVYYEWRRGRRTGTPGG"
     gene            4630..>5146
                     /locus_tag="AC16CX_14635"
     CDS             4630..>5146
                     /locus_tag="AC16CX_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR3013779.1"
                     /translation="MLKRIQSLGQWTFLRVEGLFNLAFGDRLNPLYYLGPLSYFMFWI
                     VVASGLYLYVFFKTGVTEAYESVEYLTHTQWYLGGVMRSLHRYASDAMVLTMLLHLTR
                     HFTFDRYRSFHWFSWVSGVVLLWLVYAAGINGYMLPWDRLAQFVVVATAEWFDVLPLF
                     KGALIRNFIYTE"
BASE COUNT          765 a         1686 c         1693 g         1002 t
ORIGIN      
        1 cggcttgatc tgcatcaagc cggttttgac gcatcgcagc gccgcgggcg gctccggttt
       61 ttcccgaaag cttgatctga atcaaattcc ggtgtgcggc gcctgaatta tcgttggcca
      121 gatcgaatac cgcgaatttc gctttcaaag caaaggagac cgccatggca gacgtcaccc
      181 gcccccccaa cccgtccgag atggacgagc tcgaagaggg catccccgtg atgcagcaac
      241 tcctcgacaa cccgttcctg ctcctgttcc tggggatcgc gatgcccacc gtgctctaca
      301 tcatctgggg cgtcatggag atcgtgggca tcccggtcgc gaagtagagt ccgcattctt
      361 aaccaatcct cggaggagac gccgcgatga gcgcaattca gccccctggc gcgcgggtgt
      421 ggtggaaaca gccgctggac cgcatcgagc tcgtctggat cctgctcgcc ctggtctggt
      481 gcctggtgat gttcttcatg atgccgtact ggcacgtgtt cggaaaacag aatctcgcga
      541 acgaggccta tcgcacgacg cccgaagcct acatgaagaa ggcgaccgac gtcgtcgaga
      601 agtacaccgt gcgcaaggag accgagcggc agatcccggt catccacccg cccgcgggca
      661 gcgacgtgta cctggtggcg cgcctgtggg aatggtggcc gctgctcgag ctcgaggccg
      721 gcaagagcta tcggctgcac ctcatgtcca tggactggct gcatggattc tcgctgcagc
      781 cggaaaacat caacatccag gtgcatccgg gatacgacca cgtgctcacc atcacgccga
      841 cctcgcccgg gacctactcg atcgtctgca acgagtattg cggcgtcaac caccacacga
      901 tgagcagcag gctttacgtc aagtaaaggg gggcaacgat ggcactcaat cgaatgttcc
      961 gtacctgccc cacgacgggg ctgcagttcc acgagccgtc cgaggccttg atcaaggcca
     1021 atgccgtcgc cggcgtggtg tcgctgctgg tgggcggcgt gctcgcgctg ctggtggtgc
     1081 tcacgcgctg gccggcggtg cacctgctgc ccgcggattg gttctatctc gtgctcaccg
     1141 ggcacggcct cgacatgctg gtgttctgga tcatcttctt cgagatcgcg atcctttact
     1201 tctgctcctc gacactgctg cgctgccgcc tggcgacgcc gaagatggcg tgggcgggat
     1261 tcgccctgat ggtgattggc gcgctgatga acaactttgc ggtgttcaag ggcgattcca
     1321 gcgtgatgtt cacctcgtat gcgccgatgg gcgcgcaccc gactttctac ctcgggctga
     1381 tcctgtttgc cgtcggcgcg ctgatcggct gcttcgtgtt cctcggcacg ctggtggtgg
     1441 ggaaggcgga gggtacctac aatggctcga tcccgctggt gaccttcggc gcgctcaccg
     1501 cctgcatcat tgccgtcttc accatcgcgg cgggcgcgat catcctgatc cccaccttcc
     1561 tgtggtcggt cgggttgatc gggaacatcg actcgctgat gtaccgcacc gtgtggtggg
     1621 ccttcgggca ctcctcgcag caggtcaacg tgtccgctca cgtctcggtc tggtatgcgg
     1681 tcgccgccat cgtgttcggc gcgaagccgc tctccgagaa ggtgagccgc acggcgttct
     1741 tcctctacat cctgttcctg cagctcgcct ccgcccatca cctgctggcg gacccgggcc
     1801 tcacgtcgaa ctggaagatc ttcaacacca gctacgcgat gtacctcgcg gtgctcgcga
     1861 gcatgatcca cggcttcacg gtgccgggcg cgatcgaggt ggctcagcgc gagaagggct
     1921 acaccaaggg cctgttcgag tggctgcgca aggcgccatg gggcaacccg gtgttctccg
     1981 gcatgttcat gtcgctgctc gggttcggat tcctcggcgg catctcgggc gtggtgctcg
     2041 gcaccgagca gttgaacctc atcatgcaca acaccttcta cgtgcccgga cacttccacg
     2101 ccacggtggt ggtgggcacg acgcttgcgt tcatggcgat cacctatttc ctgatcccgg
     2161 tgctgttccg gcgcgaggtc atgttcccga agctcgcgca atggcagccg tacctgttcg
     2221 gcatcggcat gagcgcgttc gcgctgttca tgatgggcgc gggcaccctc ggcgtgccgc
     2281 gccgccactg ggacatgacc atggccggca acctgatgcc gcacgaatgg ccggggactg
     2341 cgtacctgat ggtcggcttg atggcgatca gcggcttggt cgcaatcgtg ggcggcgtcg
     2401 cctacctgct gatcacggtg ggctcgatat tcttcggcaa gcgcatcgag acgcccggct
     2461 accggcacat gcgcgtcgcc gaaggccggg cggcgcccgc cccggcacag ccggtcgccg
     2521 cggcgattac cgggcatggc gggaccggca tagccgtggc gccgggaacc ttcgtgctgg
     2581 cgatggtgct gctggtgtcg ttcgttctct actacttcgt caactggaag tatctgtcgc
     2641 aggtctggcc tttgagttga ggaaagcggc gcgggacgca cggcgcaagc cgggccccct
     2701 cgcgcaacgg gtgggagaca ggcaatggcg gcaatgcggt tgtggtttgg gttgttcaag
     2761 ctccggatcg ggacgctgat cgccatcacc gcgctcgccg gagccgcggt cacgccgggg
     2821 ccgccgctca ccgcgtggca gtacgtggtg ctcgcgttga gcgtgctggc ggcctccgcg
     2881 gcggccggcg cgttcaacca gttctacgaa cgcgatctcg atctcaggat ggcgcgcacg
     2941 cgccggcgcc cgttcgtgac cggcgccctg caaccgcacg cggcgtggct ggcgctgatc
     3001 gcgttgctgc tcgccgcggc gaccgtcttc gccgcgacgg tgttgaacgc cgcgagcgct
     3061 gcatacgtgt ttctcggggc gttcgtgtac ggcgtggtgt acaccgtgtg gctcaagcgc
     3121 cgcagctggc tcaacatcgt gatcgggggg ctcgccggca gcttcgctgt gctggccggc
     3181 gccgccgcgg tgacgccggc cctggacgcg acgccgatca tcctcgcggt ggtgctgttt
     3241 ctgtggacac cgccgcactt ctggtgcctg gctatcgcct tccacaggga ctacgccgcc
     3301 gcgggagtgc cgatgctgcc ggtggtgatc ggggatgccg gcgccgcgcg ggtgatcttt
     3361 cacaatacgg tgatgctggt cgcaacatcg ttgctgccgc tcgcgttcgg catgggcgcg
     3421 ctttatgcgg ccggggccgt gctcggcggc gccttgttcc tgggacgcgg atacgtgctc
     3481 atgcaggagc cgacgccggc ggcggcgatg cgctgcttcc atgcctcgct cgcgcagctt
     3541 gcgttgctgc tcgtcggcgc gatgctggac gttgcgctgc gaagctagag gagaagccgg
     3601 ccccatgtgg tacgcggtgc tccaacctga gatgctctcg gcgctgctgg ggatttccgc
     3661 aagcgccggc gcggccctgc cgccttccgc cccgcgcgat gcggccgtcc cggttcgcgc
     3721 ggaagcgctc gccgcgccgt ttcccaggcc gatgccgctc gaaaaggcgc gcgccaccgc
     3781 cggtctcgat atggatcagg ccctgcgcgt gagccaggcc gccatgggca gggagatcgg
     3841 ggactacgca ttcttcgacc gcgaggggcg gcgcgtgcgc ctgtccgact atcgcggcaa
     3901 gccgctggtc gtgaacttcg tctacaccgg ctgctcccag gtgtgcccga ccgcgacccg
     3961 cttcctcgcg cgggcgatcc agaacgcgca gcaggcgctc ggccccgaca agttcaacgc
     4021 ggtgacgatc gggttcaacg tgccgttcga cacgccgctc gcgatgaaga atttcgccgc
     4081 gcgccacggc gtgagcctgc cgaactggga gttcctcagc ccggaccgcg agacggtggc
     4141 ggcgctcacc gccgatttcg gcttcagcta cgcgctgaca gcgggcgggt tcggccacat
     4201 cacgcagctc acgctggtgg atccgcgggg acgcatcgtg cgccagatct acggcgaggg
     4261 cttcgagctg ccgatactgg tggagccgct caagcaactc gtggccggcc ggccgacgct
     4321 ggcggacggc gccgcgcagg ccctcgacaa ggtccgcatc ctgtgcacga tctacgaccc
     4381 ggcgctcggc cgctatcgcc tgaactacgg attgttcatc gccatcctcg tcggcctgtc
     4441 ggtcctcggc agcaccggct acctggtgta ctacgaatgg cgccgcgggc ggcgcaccgg
     4501 gaccccgggc ggttgatcca catcagaccg ggcgggtatt ccacgcgtta gactgcatcg
     4561 cacgctgcag ccgtcgcacg gcagtttgcg aggcttcagc ggagatttcg tgcgtaccat
     4621 cggagcaacg tgctgaagcg tatccagtca ctggggcaat ggacgttcct gcgcgtcgag
     4681 ggcctgttca acctggcctt cggcgaccgt ttgaatcccc tgtactacct cgggccgctc
     4741 tcctacttca tgttctggat cgtggtggcg agcggccttt acctgtacgt cttcttcaag
     4801 accggcgtga ccgaggccta cgagtcggtg gaatacctga cgcacacgca gtggtatctc
     4861 ggcggcgtca tgcgcagcct ccaccgctac gcttccgacg cgatggtgtt gacgatgctg
     4921 ctgcatctca cgcgccattt cacgttcgac cgctaccgca gcttccactg gttctcctgg
     4981 gtgagcggcg tggtgctgct gtggttggtg tacgcggccg gcatcaacgg ctacatgctg
     5041 ccgtgggacc gcctggcgca gttcgtggtg gtcgcgaccg ccgagtggtt cgacgtcctg
     5101 ccgctgttca agggcgcgct gatccgcaac ttcatctaca ccgagg
//
LOCUS       DBNGWZ010000475         9454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2455167, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000475 DBNGWZ010000000
VERSION     DBNGWZ010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9454
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2455167"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="AC16CX_14640"
     CDS             <1..196
                     /locus_tag="AC16CX_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3013780.1"
                     /translation="AMRLQQDLWNLNEAFAKHNLAACIKGGLELQGYPVGVPLPPQAP
                     LSKEGMEDVRRALAAVGALE"
     gene            complement(346..1686)
                     /locus_tag="AC16CX_14645"
     CDS             complement(346..1686)
                     /locus_tag="AC16CX_14645"
                     /inference="COORDINATES: protein motif:HMM:NF023165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HIR3013781.1"
                     /translation="MLKSVILRALALLMAASAALAQDRDAFRQEELDQMLAPIALYPD
                     PLLSQILMASTYPLEVVQAARWSRSNPHLQGQDAVKAVEPMDWDPSVKSLTAFPQVLA
                     MMDEKLDWTQRLGEAFIAQQPQVMDTIQGLRQRAAAAGNLRSDERARVTRQGETILIE
                     SASPQVVYVPYYDPWLVYGPWWWPAYPPVYWAPPPGYYLRPAYAPGFFWSSGIVIAAG
                     FFFGHFDWPHRYVKVVHVAPTARSATWQHDPIHRRGVPYRHAALHQWPGHFQAFTGDT
                     RRDVRGPDGNTAVVRGHEERQAGPPVPRSNAWGDTATGQSERARLADRLTPQASERQR
                     EIRGDTARQRSLVTAPLPAARTPSAPAVTRGDGGTAGSQSHWRKPAASEGGRRPETRS
                     VRDRGNTVHQSTMAVRIPTPALRPSASHDFRSTPRMESRRQRNESRPRDGRHRQ"
     gene            complement(1957..2676)
                     /locus_tag="AC16CX_14650"
     CDS             complement(1957..2676)
                     /locus_tag="AC16CX_14650"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR3013782.1"
                     /translation="MHSIFVSDLHLAPERPRASELFLDFTARTAARAEALYVLGDLFE
                     YWVGDDDLDNEFNASVADALGALSGKGVALHFMHGNRDVLVGKAFAARCGAKLMRDPT
                     LLDLYGTRTLVMHGDTLCTDDVEYQKFRAFAHDPDNQRRLLAQPLAERRKQIVDLRAV
                     SEEAKRGKSAEIMDVAPATVEKTLREFGYPRLIHGHTHRPARHVHDVDGHTCERWVLT
                     DWYGRAGYLRCDASGCKAVAL"
     gene            complement(2781..3272)
                     /locus_tag="AC16CX_14655"
     CDS             complement(2781..3272)
                     /locus_tag="AC16CX_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3013783.1"
                     /translation="MVKLHTNHGTITIELDEKRAPQTVANFLNYIKDGHYVNTLFHRV
                     IDGFMIQGGGFEPGMRQKPTRAAVKNEADNRLKNAAYTIAMARTSEPHSATAQFFINV
                     ADNAFLDHTAPTPQGWGYCVFGRVVEGTDVVDRIKGVKTGRKGGHQDVPQEDVVIEKA
                     EVI"
     gene            complement(3340..3927)
                     /locus_tag="AC16CX_14660"
     CDS             complement(3340..3927)
                     /locus_tag="AC16CX_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR3013784.1"
                     /translation="MLRTAVAVLSLLVSFGVGAADPQVELKTTLGTIVIELFPDKAPL
                     TVENFLQYVKGGHYDGTLFHRVIPGFMIQGGGFDRDFKEKPTRKPVRNEAANGLRNEA
                     GTIAMARTSEPHSATAQFFINVADNARLNFRFPTVEGYGYCVFGKVVKGMETVRRIEK
                     VQTGPGPAPHQNVPIKPVTIESARVLGASAKPAAK"
     gene            complement(4021..5316)
                     /locus_tag="AC16CX_14665"
     CDS             complement(4021..5316)
                     /locus_tag="AC16CX_14665"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3013785.1"
                     /translation="MKLRSLLSLRPLRYTAATLLLAAACGGALVLSSRTGQAVSAASA
                     ISTVPAPGINGNPAAVGPEALLFRTLHEVSNRRLDAAISEIDKVINAYPNFRLAHLIK
                     GDLLLARARPLTTVGNAPGAPRDLLEDLRQEARARLARAQHLRPVDRVPRYLLQMQPE
                     QRQALVVDTARSTLYVFENHNGEPRYLADYYVSIGKNGIDKFREGDNKTPLGVYHVTG
                     SLSREKLDSTYGKLAEQYGVGALPISYPNEWDRREGRNGYGIWLHGVPFDTYSRPPRA
                     SNGCVALTNEDFTAISRSVQIGLTSVIIANGVDWVAPGETRALRQELAQRLESWRHDW
                     ESLDTEKYLKHYAAGFSSGKMDLAQWSDQKRRVNAGKSWIKLKLDKVSIFLYPGRDEL
                     AVVSFDQDYASSNLANQMRKRQYWIRERGTWRILHEGSA"
     gene            complement(5313..6335)
                     /locus_tag="AC16CX_14670"
     CDS             complement(5313..6335)
                     /locus_tag="AC16CX_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase Cds6 family protein"
                     /protein_id="HIR3013786.1"
                     /translation="MAHAAARLIALLATLFIALLVPAPAIAQGDELQDANQLFKQGQL
                     DRALDRVDAYLKSRPKDARGRFLKGLILAEQNKPNDAIKVFTELTHEFPELPEPYNNL
                     AVLYASQGQYDKARTALEMAIRTHPSYATAHENLGDIYAKMASQAYDKALQLDKSNTP
                     LQTKLNLIRDLFSSSPRPPKVAATRPDATKLAVATPSQKPPAPAPQPSVKAPEPTAKP
                     SPAKKEPARNADEVLKAVNQWAKAWSDNDVAGYLGYYATDFQTPKGESRSDWETQRKS
                     RIAKPRKIRVDIDTPRVVFNDSGRVTVTFRQHYRSDNLKVSSTKTLVMVKAGDKWLIQ
                     QERVGG"
     gene            6511..7944
                     /gene="cysS"
                     /locus_tag="AC16CX_14675"
     CDS             6511..7944
                     /gene="cysS"
                     /locus_tag="AC16CX_14675"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3013787.1"
                     /translation="MLKIYNSLTRDKQEFVPITPGRVRMYVCGMTVYDYCHLGHARVM
                     VVFDVVRRWLRALGFAVTYVRNITDIDDKIIKRAQESGEDVGALTARFIQAMDEDNAA
                     LGVEKPDFEPRATEHVPGMLKMIGVLEQRGLAYRAANGDVNYSVRKFPAYGKLSGKSL
                     DDLRAGERVEVDITKQDPLDFVLWKNAKEGEPFWDSPWGKGRPGWHIECSVMSSALLG
                     GHFDIHGGGQDLQFPHHENEIAQSEGANQSKFVNYWMHNGFVRVQKGEMSEKMSKSLG
                     NFSTVREVLAHYDAEVIRFFIVRAHYRSPLNYSDLHLEDAKQALTRLYTALKGFDARP
                     GAVDWSNAHAHRFREAMNDDFNTPEAIAALFDLANDLNRTRDPEHARLLKSLSGVLGL
                     LKRDPVAFLQGRVTGQGTLTSGPATVRGTGTVSHTPESIEKLISARTAARKARNFADA
                     DRIRKELLDAGIVLEDLPDGRTTWRRS"
     gene            8044..9297
                     /locus_tag="AC16CX_14680"
     CDS             8044..9297
                     /locus_tag="AC16CX_14680"
                     /inference="COORDINATES: protein motif:HMM:NF019285.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HIR3013788.1"
                     /translation="MLSVLHSAFGFVAIFLIAWLISEDRRAVPWRIVVSGALLQIVLA
                     AALLKVPLFRDLFLALNEMLSALERATQEGTRFVFGFLGGGAQPFEERPGASSFVLAF
                     RALPLILVVSALSSLLFYWRILPAIVRAASALLEKTMGVGGAVGLSAAANIFVGMVEA
                     PLFVRPYLKEMSRGELFIVMTGGMAGIAGTVMVLYATILGPVVPDAMGHILSASIVSA
                     PAAILIAAVMVPPRAAPTSGRLEPPQPATSSMDAVTRGTLDGLALLLSIVAMLIVLIA
                     LVTLVNLVLGLLPQWGDTPMTLQRLLGWLMAPFVWLAGVPWTEATTAGALMGTKTVLN
                     EFIAYVDMAKLPADALSPRSRLIMTYALCGFANFGSLGILIGGMATMVPERRDEIVAL
                     GLRSIVSGTLATLMIGAVVGMLVTE"
BASE COUNT         1496 a         2966 c         3212 g         1780 t
ORIGIN      
        1 cgcgatgcgg ctccagcagg atctgtggaa tctcaacgag gcgttcgcaa agcacaatct
       61 tgcagcctgc atcaaaggcg ggctggaatt gcagggctac cccgtcggcg tcccgctgcc
      121 gccacaggca ccgctgtcaa aagagggcat ggaagacgta cggcgcgcgc tcgcggccgt
      181 cggcgctctt gagtaaccga ttcgatcttc ctccgcgttt ttatatgttg atccccgctg
      241 gcagtaagcc agcaaccgcg gacgcccggc ggaaatggct gccgcacagt ggccgagcca
      301 gagccggccc ggagcttttc gctgttaact ccctcccccc gcgggttact gccggtggcg
      361 gccgtcacga ggccgtgact cgttgcgttg acgccggctt tccattcggg gcgtggaacg
      421 gaaatcatgg ctcgcggagg gccggagcgc aggcgtaggg atgcgcaccg ccattgtgct
      481 ttgatggacg gtgttcccgc ggtcccggac gcttcgtgtt tccggccggc gcccgccctc
      541 tgatgcagca ggcttccgcc agtgactttg gctgccggcg gtaccgccat cgccgcgcgt
      601 cacggcggga gccgagggcg ttcttgctgc gggtagtggc gcggtgacca gagagcgctg
      661 ccgtgcggta tccccgcgaa tttcccgttg cctttcactt gcctgtggcg tgaggcgatc
      721 cgctagccgc gcgcgctccg actgtccggt ggcggtgtcc ccccacgcat tcgaacgtgg
      781 gaccggcgga cccgcctgac gttcctcgtg tccccgcacc acggccgtgt tcccgtcggg
      841 cccgcgtacg tcgcgccggg tgtcgccggt aaatgcctgg aaatggccag gccactggtg
      901 gagtgccgcg tgccggtagg gaacgccccg gcgatggatc gggtcgtgtt gccatgtcgc
      961 cgaccgcgca gtcggcgcga cgtgcacgac cttcacgtac cggtggggcc agtcgaaatg
     1021 gccgaagaaa aatcccgccg caatcacgat accgctgctc cagaagaagc ccggtgcgta
     1081 cgcgggcctg aggtagtacc ccggtggggg cgcccaatag acaggcggat aggccggcca
     1141 ccaccacggc ccgtagacga gccacggatc gtagtacggg acatagacga cctgcgggct
     1201 ggcagactcg atgaggatgg tctcgccctg ccgcgtgact cgcgcccgct cgtcggaccg
     1261 gagattgccc gcggccgccg cccgttgacg caggccctgt atggtgtcca tgacctgcgg
     1321 ctgctgcgcg atgaacgcct cgcccagccg ctgcgtccag tcgagcttct cgtccatcat
     1381 ggcgaggacc tgcgggaacg cggtcagcga cttgacgctg ggatcccaat ccatcggctc
     1441 caccgccttc accgcgtcct gcccctgaag gtggggattg gaccgcgacc agcgcgcagc
     1501 ctgcaccacc tcgagggggt aggtggacgc catcaggatc tgcgacagca gcggatcggg
     1561 atacagcgcg atcggggcca gcatctggtc gagctcttcc tgcctgaacg cgtcgcgatc
     1621 ctgcgcgagc gcggccgatg ccgccatcag cagggcgagc gctcgcagga tcaccgactt
     1681 cagcatcgtt ttcaccgctg tcacctcgtg tcatcgtgcc gtcccgcccc cgggcggcct
     1741 gttttccggg ggcgatgggc aaccacttga gggtacgaac gcggcagggc cgccggcgtt
     1801 cacaatcttc cccgtttccg gcgttacaag atgtaaaagt ttgtaagccg cggtgaagcc
     1861 gccgtcggtg tggccgccga gagagggggg tgaggagcga aatgggacgg ggcgtgccgc
     1921 caaacccggc cggagtcagg agcggtcgcc gcccggctac agcgccacgg ccttgcagcc
     1981 gctggcgtcg cagcggagat agccggcgcg cccgtaccag tccgtcagca cccagcgctc
     2041 gcaggtgtgg ccgtccacgt cgtgaacgtg gcgtgcgggg cggtgggtgt ggccgtggat
     2101 gaggcgggga tagccgaact cccgaagcgt cttctccacg gtggcgggcg cgacgtccat
     2161 gatctcggcg cttttcccgc gcttcgcttc ctcgctcaca gcccgcaggt caacgatctg
     2221 cttccggcgc tccgcgagcg gttgggcgag gagcctgcgc tggttatccg ggtcgtgcgc
     2281 aaacgcgcgg aacttctggt actcgacgtc gtcggtgcac agcgtgtcgc cgtgcattac
     2341 gagcgtcctc gtgccgtaga ggtcgagcag ggtggggtcc cgcatcagct tcgcgccgca
     2401 gcgcgccgcg aacgccttgc cgaccagcac gtcgcggttg ccgtgcatga aatggagcgc
     2461 gacgcccttt ccgctcaatg cccccaacgc atcggcgacc gaggcattga actcgttgtc
     2521 cagatcgtcg tccccgaccc agtattcgaa cagatccccg aggacgtaga gcgcctcggc
     2581 ccgcgccgcg gtgcgcgcgg tgaaatcgag gaacagttcg ctcgcgcgcg gccgctcggg
     2641 agcgaggtgc aggtccgaga cgaaaatgct gtgcattttc gtctaagtcg gaaggcggaa
     2701 ggcgaaaggc gaaaggacgc ggccagccgc ccggtcaaga ccgtatctta ctttcgactt
     2761 tcgcctttcg actttcgcct tcagatgacc tccgctttct cgatgacgac gtcctcctgc
     2821 ggcacgtcct ggtgcccgcc cttgcggccg gtcttgacgc ccttgatcct gtccacgacg
     2881 tcggtgcctt ccaccacgcg cccgaacacg cagtagcccc agccttgcgg cgtgggggcg
     2941 gtatggtcga ggaacgcgtt gtcggcgacg ttgatgaaga actgcgcggt cgcggagtgc
     3001 ggctcggaag tgcgcgccat ggcgatggtg taggccgcgt tcttgagccg gttgtccgcc
     3061 tcgttcttca cggcggcgcg cgtgggcttt tgccgcatcc cgggctcgaa gccgccgccc
     3121 tgtatcatga agccgtcgat gacccggtga aaaagcgtgt tcacgtaatg accgtccttg
     3181 atgtagttga ggaagttggc caccgtctgc ggagcgcgct tttcatcgag ttcgatcgtg
     3241 atggtgccgt gattggtgtg aagtttgacc atataaatcg ggtgatgagt gatgggtgat
     3301 gggtgatgag tcaaacccgg aagccgggtc gcgccacatt catttcgcgg cgggtttcgc
     3361 ggacgccccc aggactctcg ctgattcgat ggtgaccggc ttgatcggga cgttctggtg
     3421 aggcgccggt cccggaccgg tctgcacttt ttcgatgcga cggaccgtct ccatgccctt
     3481 caccaccttc ccgaacacgc agtagccgta accctccacg gtgggaaagc ggaaattgag
     3541 acgggcattg tccgccacgt tgatgaagaa ctgcgcggtc gcggagtgcg gctcggaagt
     3601 gcgcgccatc gctatcgtgc ccgcctcgtt gcgcaggccg tttgcggcct cgttgcgcac
     3661 cggcttgcgc gtcggcttct ccttgaagtc ccggtcgaat ccgccgccct ggatcatgaa
     3721 gcccgggatc acgcggtgga aaagggtccc gtcgtagtgg ccgcctttca cgtactggag
     3781 gaagttttcc accgtgagcg gcgccttgtc ggggaacagt tcgatgacga tggtccccag
     3841 ggtggtcttc agttccacct gcggatccgc cgctccgact ccgaaggaga cgagcaggga
     3901 aaggactgcg acagcggttc tcagcatgtg tctgagagcg gcggatcaat aaatgggaag
     3961 cgcagcatcc gctgcgtcat gccctcaacc atctaacagg ttcattgatg aaggcagcgc
     4021 tcaggcgctg ccttcatgca ggatgcgcca cgtgccgcgt tcccggatcc agtactggcg
     4081 cttgcgcatc tggttggcga ggttgctgct cgcgtagtcc tggtcgaagc tcaccaccgc
     4141 gagttcgtcc ctgccggggt agaggaagat gctgaccttg tcgagcttga gcttgatcca
     4201 cgacttgccg gcgttgaccc tgcgcttctg gtcggaccac tgcgcgagat ccatcttgcc
     4261 ggaggagaag ccggcggcgt agtgcttgag gtatttctcg gtgtcgaggc tctcccagtc
     4321 gtggcgccag ctttcgagcc gctgtgcgag ctcctgccgc agcgcgcggg tttcccccgg
     4381 cgcaacccag tccacgccgt tggcgatgat cacggacgtg agcccgatct gaacgctcct
     4441 tgagatcgcc gtgaagtctt cattggtgag cgccacgcag ccattgctcg cgcgcggcgg
     4501 ccggctgtag gtgtcgaagg gcacgccgtg cagccagatg ccgtagccgt tgcggccttc
     4561 ccggcggtcc cattcgttgg gatagctgat cggcagcgcg cccaccccgt actgctcggc
     4621 gagcttgccg taggtcgagt ccagtttttc acgcgagagg ctgcccgtga cgtggtagac
     4681 gccgagcggc gtcttgttgt cgccctcccg gaacttgtcg atgccgttct tgccgatgct
     4741 gacgtagtaa tcggcgaggt agcgcggctc gccgttgtgg ttctcgaaca cgtagagcgt
     4801 ggacctggcg gtgtcgacga cgagggcctg gcgctgctcc ggttgcatct gcagcagata
     4861 acgcggcacg cggtcgacgg gacgcaggtg ctgggcgcgc gccagccgcg cacgcgcctc
     4921 ctggcgcagg tcctcgagca ggtctctcgg cgccccgggc gcattgccga cggtggtgag
     4981 cggccgcgcc cgggcgagca gcaggtcgcc cttgatgagg tgcgccagcc tgaagttggg
     5041 gtacgcgttg atgaccttgt cgatctcgga gatggcggcg tcgagccgcc tgttgctgac
     5101 ttcgtgcagc gtcctgaaca acaacgcttc gggcccgacg gcggcggggt ttccgttgat
     5161 gccgggtgcc ggcacggtgg aaatggcgga agccgcggaa acggcctgtc cggtgcggct
     5221 gctcaggacg agcgcgccgc cacacgcggc tgccagaagc agcgtcgcgg ccgtgtaacg
     5281 caacggacgc aagctgagca gcgatcgcag cttcaaccgc caaccctttc ctgctggatg
     5341 agccacttgt ctcccgcctt gaccatcacc agcgtcttgg tgctggaaac cttgaggttg
     5401 tccgagcggt aatgctggcg gaaggtcacc gtaacgcgtc cgctgtcgtt gaacaccact
     5461 ctgggcgtgt cgatatccac ccggatcttg cgcggcttgg cgatgcgcga cttgcgctgg
     5521 gtctcccaat cgctgcgcga ttcgcccttc ggggtctgaa agtccgtcgc gtagtacccg
     5581 aggtagccgg cgacgtcgtt atccgaccac gccttggccc actggttgac cgctttcagc
     5641 acttcgtccg cgttgcgcgc gggctccttc ttggccgggc tgggcttggc agtcggttcc
     5701 ggcgccttga ccgacggctg cggagccggc gccggcggct tctgggaggg ggttgcaacc
     5761 gcaagcttgg tcgcgtccgg ccgggtcgcg gccaccttcg gcgggcgtgg gctgctcgag
     5821 aagaggtccc tgatgaggtt gagcttggtt tgcagggggg tattgctctt gtcgagctga
     5881 agcgccttgt cgtaggcctg gctcgccatc ttggcgtaga tgtcgccgag gttctcgtgc
     5941 gcggtggcgt agctggggtg ggtgcggatc gccatctcaa gcgcggtgcg ggccttgtcg
     6001 tattggccct gcgaggcgta gagcaccgcc aggttgttgt acggctccgg cagctcggga
     6061 aactcgtgag taagctcggt gaagaccttg atcgcgtcgt tcggcttgtt ctgctcggcg
     6121 aggatcagcc ccttgagaaa acgcccgcgc gcgtccttgg gccggctctt gagataagcg
     6181 tcgacgcggt cgagcgcgcg gtcgagctgt ccctgcttga acaactgatt ggcgtcctgc
     6241 aactcgtcgc cttgagcgat cgccggcgcc gggacgagaa gagcgatgaa cagcgtcgca
     6301 aggagcgcga tcaggcgggc ggcggcgtga gccattgtgt tatacttttc cgagaatttc
     6361 agtggcttag tcgctaaatc ctagaaattt taccaagaac gccgggcaat tcaaactgcg
     6421 catttccgcg cgccgcctgc gccctcccgc cagtcgggcc ggtgagcccg cggataccgg
     6481 ccaggacgcc ctgagggacg ggcgcaagcc atgctgaaga tctacaactc gctcacccgc
     6541 gacaagcagg aattcgttcc gattaccccg ggccgggtcc gcatgtacgt ctgcggcatg
     6601 acggtctacg actactgcca tctcgggcac gcgcgggtga tggtggtgtt cgacgtggta
     6661 cggcggtggc tgcgcgcgct gggcttcgcg gtcacctacg tgcgcaacat caccgatatc
     6721 gacgacaaga tcatcaagcg cgcgcaggag agcggcgagg atgtcggggc gctcaccgcg
     6781 cgcttcatcc aggcgatgga cgaggacaac gccgcgcttg gggtggagaa gcccgatttc
     6841 gagccgcgcg cgaccgagca cgtgcccggc atgctgaaaa tgatcggggt tctagagcaa
     6901 agaggcctcg cctaccgggc ggcgaacggc gacgtcaact actccgtgcg caagtttccg
     6961 gcctacggca agctctccgg caagtcgctc gatgacctgc gcgccggtga gcgcgttgaa
     7021 gtggatatca cgaagcagga ccccctcgat ttcgttctgt ggaaaaacgc gaaggagggc
     7081 gagccgttct gggactcgcc gtggggaaag ggccgcccgg gctggcacat cgagtgctcg
     7141 gtgatgtcga gcgcgctgct cggcggccac ttcgacatcc acggcggtgg ccaggacctg
     7201 cagtttcccc atcatgagaa cgagatcgcg cagtccgagg gggcgaacca gtccaagttc
     7261 gtcaactact ggatgcacaa cggcttcgtg cgcgtgcaga aaggggaaat gagcgagaag
     7321 atgtcgaaat cgctcggcaa cttctccacc gtgcgcgaag tgctcgcgca ttacgacgcc
     7381 gaggtgatcc gcttcttcat cgtccgcgcg cactaccgca gcccgctcaa ctattccgac
     7441 ctgcacctgg aggatgcgaa gcaggcgctg acgcgcctct acacggccct gaagggcttc
     7501 gacgccagac cgggcgcggt ggactggagc aatgcccatg cccaccgttt ccgcgaggcg
     7561 atgaacgacg acttcaacac gccggaagca atcgccgcgc tgttcgatct cgcgaacgac
     7621 cttaaccgga cgcgagatcc ggagcacgcg cgattgctta aatcactcag cggcgtcctg
     7681 ggattgctga aaagggatcc agtcgctttc ctgcaggggc gcgtgacggg tcaagggacg
     7741 ctcacatcgg ggccggcaac tgtgcgcggt acggggaccg taagtcacac acccgaatcc
     7801 attgagaagc tcataagtgc ccgcacggcc gctcgtaaag cgagaaactt cgccgacgcc
     7861 gaccgcatcc ggaaggagct gctcgatgca ggaatcgtgc ttgaggattt gcccgacggc
     7921 cggacgacat ggcggcgtag ctagaccttg aatccgcgtg ttgccgggtt tgttgatgca
     7981 ggacgggttt gatcccggaa aaaactgcgt taagcgtgat ctttagtgga ggtgagaaaa
     8041 cgcgtgttat ctgtcctgca tagcgctttc ggcttcgtcg ccatcttcct gatcgcctgg
     8101 ctcatctccg aggaccgccg cgcggtgccg tggcgcatcg tcgtctccgg cgcgctgttg
     8161 cagatcgtgc tggcggcggc gctcctcaag gtcccgctgt tccgggacct gttcctcgcg
     8221 ttgaacgaga tgctctccgc gctggaaagg gcgacgcagg aaggcacgcg cttcgtgttc
     8281 ggctttctcg gcggcggggc gcagccgttc gaggagcgcc ctggggcttc gagctttgtg
     8341 ctcgccttcc gcgcgctgcc gctgatcctc gtcgtgagcg cgctgtcttc gctgctcttc
     8401 tactggagaa tcctgcctgc catcgtgcgc gcggcctccg cgctgctcga gaagacgatg
     8461 ggggtgggcg gggcggtggg tctgtcggcc gcggccaaca tcttcgtcgg catggtggag
     8521 gcgccgctgt tcgtccgccc gtacctgaag gagatgagcc gcggtgagct gttcatcgtc
     8581 atgaccggcg gcatggccgg catcgccggc acggtgatgg tgctgtacgc gacgatcctg
     8641 ggaccggtgg tgccggatgc gatggggcac atcctctcgg cttccatcgt cagtgccccg
     8701 gcggcgatcc tcatcgcggc agtcatggtg ccgccgcgcg cggcgccgac ttccgggagg
     8761 ctcgagcccc cgcagccggc gacgagcagc atggacgcag tgacccgcgg cacgctcgac
     8821 ggtctcgcgt tgctgctcag catcgttgcg atgctgatcg tgctgatcgc gttggtcacg
     8881 ctcgtcaacc tcgtgctcgg cctgctgccc cagtggggcg atacgccgat gacgttgcaa
     8941 cggctgctgg gctggctgat ggcgccgttt gtctggctcg cgggtgttcc gtggaccgag
     9001 gcgaccacgg ccggcgcgct gatgggcacc aagaccgttc tcaacgagtt catcgcctac
     9061 gtggacatgg cgaagctgcc ggcggacgcc ttgagcccgc gcagccggct catcatgact
     9121 tacgccctgt gcggcttcgc caatttcggc agcctcggca tcctgatcgg cggcatggcg
     9181 accatggtgc cggagcggcg cgacgagatc gtcgcgcttg gcctgcgctc gatcgtctcc
     9241 ggcacgctcg ccacgctcat gatcggcgcg gtggtcggca tgctcgtcac cgagtaacga
     9301 gtgacggggc gcgccgaggg gcggagcagg attccatcgg cttttgccga tggatgcgac
     9361 ccccggtggc gcgcagggcg gccgacatga ttgagcagat atctagcgat gacatgtcgg
     9421 aggctgccgt cgagtgacgg agtgagggag gggg
//
LOCUS       DBNGWZ010000476        14233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1323527, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000476 DBNGWZ010000000
VERSION     DBNGWZ010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1323527"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="AC16CX_14685"
     CDS             <1..487
                     /locus_tag="AC16CX_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013789.1"
                     /translation="KACPGRVMGVNFAGTNDQGVKIKTDMAMRMKQRFEKHLDRIPPD
                     PEIRQALLAIKRETTGTAIRFDAPRIEVDSPVAGGEKRKRFGHADEFWAKAMASLAAA
                     GAPAAAFAGADADRSWYHGEARGAMRAAAEAQRVRELPEEESPSAPLVVRERRSALGI
                     R"
     gene            484..1980
                     /locus_tag="AC16CX_14690"
     CDS             484..1980
                     /locus_tag="AC16CX_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HIR3013790.1"
                     /translation="MSFVLLNLGEARKAERARALARNDGEVRLTRAAISPQLFDLGAA
                     ADERGFRRITSPATLRDLNPLMQERMQQVCFFLAVTTPFGKRIVEIITSYVIGEGFQL
                     AAEDPAAQEVLDRFAKDPINNLRGNLREWCRELAIFGELCLPTAVNPVDGFVRLGYVD
                     PQEIDAIAFGGLQTARAQELSIPVAVRLRQRIGESEGRRLAIVRAQEDSLSEDYGRLS
                     GECFYLAINKAKSASRGISELFSLADWIDVFDQMVFDFADKVRFLNTFVWEFVLKGAD
                     QAAVEAFKKAMLKNPPRQGGFQVHNESVEMKPQTPDLKSADMHEAARMVKLYGLGGAG
                     LPAWFFADPMDANRSTAIEMEGPTGKKLTDWQNVLRELVHQIGAYALDQAVGHGVLPE
                     GGDLNFKVIAPDMSIRDMTKAASVLQVGGNALALAEDRGWIRKETAARAFHTALGQLG
                     VEVESKKEFEEAQQEREDRMAQDVNQFAPQKNLADALKQLPPAKETVQ"
     gene            1977..2867
                     /locus_tag="AC16CX_14695"
     CDS             1977..2867
                     /locus_tag="AC16CX_14695"
                     /inference="COORDINATES: protein motif:HMM:NF016148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HIR3013791.1"
                     /translation="MSQQSDFAAKVHELVRQSQQLSPEARRQVLELLDEARKRILGEL
                     ATLDPANFQAAQLRTLKLSIDSAFDNFRAEATRAVNGLQQRGYSFGQAAINEPLDAAG
                     LPSFAGVSRGTLAIAQGYTADLISGLAKDSAAKVNAAIQRAFLGGQSLTDIIGQIGKA
                     LAGGDFTGLFSPIGERATTIAINEVLRVHSIAAQERLDQAADHLPGLKKRWRHVPAAR
                     MPRITHIEADGQVREVDEAFEVGGEELMYPRDPAGSPENTINCHCLTEPYFEPEALEP
                     TEKQRGLLSDLGIEISVAPR"
     gene            2886..3251
                     /locus_tag="AC16CX_14700"
     CDS             2886..3251
                     /locus_tag="AC16CX_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013792.1"
                     /translation="MADEAKIDIPEVPPVPGLPEQVKAAWRKAYADAFKRAQVDHKDD
                     PLMQRQVALGEANRIFRLDAPVDYDDAMDLAPWQVIKREAREGELKVVTIDGQKFSFP
                     VPAPKPAAVAGPTRGGEKK"
     gene            3335..5851
                     /locus_tag="AC16CX_14705"
     CDS             3335..5851
                     /locus_tag="AC16CX_14705"
                     /inference="COORDINATES: protein motif:HMM:NF051749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR3013793.1"
                     /translation="MAKTKMVDGRELTASDFAFVGNPDDPETWKLPVHDEGHARAALG
                     RFNQTDMPADAKAATARKIVAACKKFGIDASGFMEEHKAMMAASSWFAVSQAAQFLAE
                     EGAGAEEGYVWPVQIIEAGRAQGSLVDGDGKSAGKTPHVFTEEVVAQFAAAANGARFG
                     RRHPAALESDDDPARIAGWFSEGVVAGKAARAKLHLLKNEQALRDKLLAAKDAGQLGL
                     FGLSVLAYVAFQRGKHDDLDALVSERLGKLISVDLVTEAGAGGKFLVAASKSLAAGLR
                     ELVESDEVKNQAAADAQTAAETAALKAQEAAAAAAAAARAAEANAKRALPVTNRQENG
                     AAPTARGGRNMGGAMKERILKVLETLRKFDSGRADELSAEFNALPEEQHVEFLGKVKE
                     ALDSVDEAQRTRGAEAATALEKRLAKAIREDKLIEAKLPAEAANLVREHLDGLGRVYT
                     EAEIDAEIKRVRTAFASFSNVGRVTEGSGITVGRDSREKVQLAMDAMLGVKEARADKS
                     VRAFRGIREAYGWITGDHELTFGNGGFGRVSEAIATADFPNILLNSLTKKLLEDYTEL
                     GMGGLELLISPADISDYKTQDRVRMGYLGDLATVAEAGAYLEITKPTDEKIQYAVGKR
                     GGLLTISEETIRNDDTGKIRQFPNRLARAARRTLKQFITDFFVNNPNFDPDTLAWFVA
                     GHSNLGSTAFSDVELDAREIALMKQTEKDSTKRLGLPLEWIMIPVDLKSAAYQVNNNN
                     DGANSWYHRFGMNETKPERIIVNELLTDANDWFGGCLPQTAPFLEIGFLDGRQEPQIL
                     LANDPQTGLAFTNDQVVYKAKFVFGGDITDYRPVFKNVVP"
     gene            5971..6369
                     /locus_tag="AC16CX_14710"
     CDS             5971..6369
                     /locus_tag="AC16CX_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013794.1"
                     /translation="MPRDRQHARTFVVALPIGDGALTAATDKAIWPVRKPCRLLECSL
                     SLRATGGTSGNTDVDVNKNGTSILAAPGLRIAQGATTKYVNTKPTGAAGMPGGVALKP
                     GDVVSVDVDAIPGAASTGGLVLLHCEATDI"
     gene            6405..7103
                     /locus_tag="AC16CX_14715"
     CDS             6405..7103
                     /locus_tag="AC16CX_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013795.1"
                     /translation="MAKSLDQFRQELALRVQDAAAKLSQEARDAMIAQAILQRYSKDR
                     PAEKVSDVDGNGTASLATPAGFEDGFSIVRSVEYPIGKVPPALLEDEGWMMYRTPTAL
                     QILLTSARPAASEKLRVTWTARHNADGSTVPEPDFEAVCDYAAALAFEALAAIHAQTG
                     DSTLMADAVNYRTKSQEYLGLAKAARKRYFDHIGVSEDRAGSPAGGPAMSIGDLDQLM
                     GSGVDRMTHPKGSR"
     gene            7100..7633
                     /locus_tag="AC16CX_14720"
     CDS             7100..7633
                     /locus_tag="AC16CX_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013796.1"
                     /translation="MSPVIRITVKGGEEAVAAIQTAVREGVTGGLEAAGLRGEQLVKE
                     SILAPPFGPPVVATGTLVNSITHQLEANDVLAAREVVFAQAPADQYAAYVEAGTGPHF
                     PPPRALLLWVKKKFGIRTEKEALSAAFAVARAIARRGTLARGVFHAAFGNLTAELGGI
                     FSRAIGAALEKAGAAGR"
     gene            7634..8089
                     /locus_tag="AC16CX_14725"
     CDS             7634..8089
                     /locus_tag="AC16CX_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013797.1"
                     /translation="MALKDIIAEVAAILATVPDIGRVHAYRRAVAAQTDIEAVFKDVV
                     TGQIRGWEVSREATASQDMLVAGTRERHQIVVYGYMSINDAKRSEETFQELVEAVRDA
                     FRVKRNLNGKAFDTTPMSARTVTAAMKAGVLVHYCELTMEAQQFPVTTA"
     gene            8162..8413
                     /locus_tag="AC16CX_14730"
     CDS             8162..8413
                     /locus_tag="AC16CX_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013798.1"
                     /translation="MARDDYVEIRLTKKGAEAAGVGTVRIVNALVDVEVRAGETHRVS
                     RAEWTEIFSLARLDGEPLFEITAAAEPAKPAMRQKAERS"
     gene            8417..9412
                     /locus_tag="AC16CX_14735"
     CDS             8417..9412
                     /locus_tag="AC16CX_14735"
                     /inference="COORDINATES: protein motif:HMM:NF039819.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR3013799.1"
                     /translation="MATFAAQQIGAREIVISPNKQNAYGTVLGDVSLVHNLRFDGGAF
                     ADLAKEYMTDMDLAKGHPWATFKNPIRQNMRLSLGGLQVYDFLAGWLAAFLFGKVQTS
                     GVGPYTHLFTADLSTTIAPVTTAWIHDTADVKSKVPDLAINSLRFNGAAIGPVTADAE
                     LVGSGRFTDIAMAAPALAVPTYLLNSDADILLGPQGAPVSIKERVRSWSVSLTANLED
                     YRHPGSGLYAGFTRRGDLRITVQLGIAAKDVDDIRTLGVLDTKRELQINVNSGAAAQL
                     KFKFPAVILRVTGGAEGTFVLWTIEADDTGVLKDGVNEPIEVTAINGQATYLVGA"
     gene            9623..10411
                     /locus_tag="AC16CX_14740"
     CDS             9623..10411
                     /locus_tag="AC16CX_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013800.1"
                     /translation="MEEALATTVGIPTERKAEALLPLDLAERAVTWSANGRVRTHVFR
                     RLTAADWEDYIAHIRFEVETGAPPRIDVTVAGLRLYERAVLLAEGYPVRGDAKLESLP
                     RWKDLLPIGERLQAVRLLMQVDRHEGDDGVMIEPDIQIAALDAIWGITGPGRTQTYRG
                     LIHRFTPPTGEHLARYSREISRAKIIGGSRTGLTVYQPREKILVKLYDELIQSVEGYS
                     VRGEPLTDREATVREMDPFHKAAAASIIFDLLGRRDEEEPEDRP"
     gene            10408..10806
                     /locus_tag="AC16CX_14745"
     CDS             10408..10806
                     /locus_tag="AC16CX_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013801.1"
                     /translation="MIEVWRDPAGLRTAMAEMLENSLRARVRRSLAQRMKGATNADRA
                     LAGLEDHRTLADGYYAWAGHLVLLDRVVRQAAPRTALRIEELRGLADLGGVEAEFERA
                     HPRCPRCGEMQTDAAELRCWNCGKEFGAPR"
     gene            10951..13398
                     /locus_tag="AC16CX_14750"
     CDS             10951..13398
                     /locus_tag="AC16CX_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013802.1"
                     /translation="MRKQQEQARDAAQLLSRAVGAEVPRALEKVLARSNTVGPALATA
                     FNAAVVTSFGVAVIAAVDKLFGFSEGLARTRRQADELFAAVAKANEILAGPKTLQGVN
                     AQIRATESSINAAQATIEVLKQILGGGLRGELTARGLQTLGILEDLNGELDKQKKLLN
                     EQYVSQAQLMDQQMREEPVEVLKRENEARLAGMVGIGKIRAEEAMTLDLLEKQLKARI
                     ITLPIYQAEVNAAHAKAKAELEAYQDLQRGMTAAAVAARNAAVLRERMPVAELPPAIT
                     ELPKWMQEMADQLDKEWASEKEIRGRQMELAGELADAESRAAIASLEPWQKAYAQIQG
                     DAERATREIAQRYGGLSEEADKAARLTTAVWAEAGLRMRDQLAGDLESAFDDITSGNI
                     GKRFLENFKKMVFQMVATWMLGMQQMRTASGFSFGSFLSGLFGFSLPGAGGAATVGAA
                     AAGAAPAFSWTAENALATSGALPLNLGGRGTSGVSLSMAALPLSAGQGAGAGIGAALP
                     AGAAVVGAAGAAKTSWLAALGGPAGLAAMGGMFGIGLLGNRSTLARGIGGALTGATAG
                     LFGGAVLASLALGIPLSAGLAVSLAAAPFTLGLSALVGGLLAVFGFGGPHKGDTARIQ
                     VMEPAQRQIKNILDEYNLFQTTLDQAISQLEQLRSQGIERLSQIGGRQKSSNSATLTR
                     WVDEAENQMKSTEAERQRRVALQFGGAMFHSGGYVNPSLARQTVPAWAAGAPRYQSGG
                     AVPALLEPGEYVVRSQAVQRIGRGRLEQMNAGGEGGGITIHINALDARSFSAWLRDGG
                     MKEMVRELRRARAEGAF"
     gene            13399..14211
                     /locus_tag="AC16CX_14755"
     CDS             13399..14211
                     /locus_tag="AC16CX_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013803.1"
                     /translation="MSEQNILNPSATSLLNYDWGYSEGLPELRSVWQAQSGKIYSRLQ
                     MARGRVYDLTWNNRDLATMHALRQWEEQYRNDFFTLADWERGRYFSGRFEGPLAFNPA
                     GFEKWNIRGRFVELPGLAMYAYPSNWARDAIFYEERDGFGADLVKLAGTWIFSSSPGV
                     FHGDAAGRYYSVTTSDTAEWIFLGYGWRVWANTENDAGIMEMKVTKVRDGSVVANANI
                     DLYSAATQPASALWTGVGIPLDLYRVRLRITGTKNPLSSNYCGFADAVEVLR"
BASE COUNT         2786 a         4030 c         5333 g         2084 t
ORIGIN      
        1 caaggcgtgc cccgggcgcg tgatgggagt gaactttgcc ggcacgaacg accagggcgt
       61 gaagatcaag accgacatgg ccatgcggat gaagcagcgg tttgagaaac atctcgaccg
      121 cattcccccg gacccggaga tccgccaggc gctgctggcg atcaaacggg aaaccaccgg
      181 aacagcgatc cggttcgatg cgccgcgaat cgaggtggac tcgccggtgg ccgggggcga
      241 aaagcggaag cgtttcggac acgccgacga gttctgggcg aaagccatgg cgagtctggc
      301 ggcggcggga gcgccggcgg cggcgtttgc gggagcggac gcggaccggt cgtggtacca
      361 cggcgaagcg cgcggggcga tgcgcgcggc ggccgaggcg cagcgcgtgc gggaattgcc
      421 ggaggaggag tcgccgtcgg cgccgctggt ggtccgcgag cggcgaagcg cgctggggat
      481 tcgatgagtt tcgtgctgct caatctcgga gaggcgcgga aagccgagcg ggcgcgcgca
      541 ctggcgcgca atgacgggga ggtgcggctg acgcgagcgg cgatctcgcc gcaactgttc
      601 gatctgggcg ccgcggcgga cgagcgcggg ttccggagaa tcacctcgcc ggcgacgctg
      661 cgcgatttga atccgctgat gcaggagcgc atgcagcagg tgtgtttctt tctggcggtg
      721 acgacgccgt ttgggaagcg gattgtcgag atcatcacga gctacgtgat cggcgagggt
      781 tttcaactcg cggcggagga tccggcggcg caagaggtgc tcgaccgatt cgcgaaggac
      841 ccgatcaaca acctgcgggg caacctgcgc gagtggtgcc gcgagctggc gatcttcggg
      901 gaattgtgcc tgccgacggc ggtgaatccg gtggacggtt tcgtgcggct cggctacgtc
      961 gatccgcagg agatcgacgc catcgcgttc ggcggcttgc agacggcgcg ggcgcaagaa
     1021 ctctcgattc cggtcgcggt gaggttgcgg cagcgcatcg gggagagcga agggcggcgg
     1081 ctggcgatcg tgcgggcgca ggaggactcg ctctccgagg attacgggcg gctgagcggc
     1141 gagtgcttct acctggcgat caacaaggcg aagagcgcaa gccgcgggat ctcggaactg
     1201 ttctcgctgg cggattggat cgacgtgttc gaccagatgg tgttcgactt cgccgacaag
     1261 gtgaggttcc tgaacacgtt cgtctgggag ttcgtgctga agggcgcgga tcaggcggcg
     1321 gtcgaggcgt tcaagaaggc gatgctgaag aatccgccac ggcagggcgg tttccaggtg
     1381 cacaacgagt cggtcgagat gaagccgcag accccggacc tgaagagcgc ggacatgcat
     1441 gaggcggcgc ggatggtgaa actctacggc ctgggcggcg cgggcttgcc cgcgtggttc
     1501 ttcgccgatc cgatggatgc caaccggtcc acagcgatcg agatggaagg accgaccggg
     1561 aagaagttga cggactggca aaatgtgttg cgcgaactgg tgcaccaaat tggggcctat
     1621 gcgctggacc aggcggtcgg gcacggcgtt cttcccgagg gcggcgatct gaacttcaaa
     1681 gtgatcgcgc cggacatgtc gatccgcgac atgacgaagg ccgccagcgt gctccaggtc
     1741 gggggcaacg ccctggcgct ggccgaggac cgggggtgga tccggaagga gacggcggcg
     1801 cgggcgttcc acacggcgct cggtcaactc ggggtcgagg tggaatcgaa gaaggaattc
     1861 gaggaggcgc agcaggagcg cgaagaccga atggcgcagg acgtcaacca gttcgcgccg
     1921 cagaagaacc tggcggatgc gctcaagcaa ctgccgccgg cgaaggagac ggtgcagtga
     1981 gccagcagag cgacttcgcg gcgaaggtgc acgagctggt gcggcagtcg cagcagcttt
     2041 cgccggaggc gcggcgacag gtgctggagc tgctggacga ggcaaggaag cggatcctgg
     2101 gtgagctggc gacgctggat ccggcgaact tccaggcggc gcagttgcgg acgctgaaat
     2161 tgtcgatcga ctcggcgttc gataattttc gcgcggaggc aacgcgcgcg gtgaacggcc
     2221 tgcagcagcg cggatattcg ttcgggcagg cggcgatcaa tgaaccactc gatgccgccg
     2281 ggttgccgag cttcgcggga gtctcgcgag gcacattggc gatcgcacag gggtacacgg
     2341 cggacctgat ctcggggctg gcgaaagact cggcggcgaa ggtgaacgcg gcgatccagc
     2401 gagcgtttct cggcgggcag tcgctcaccg acatcatcgg ccagatcggg aaggcgctgg
     2461 ccggcggaga tttcaccgga ctgttctcgc cgatcggaga gcgcgcgacg acgatcgcga
     2521 tcaacgaggt gctgcgagta cattcgatcg cggcgcagga aaggctcgac caggctgcag
     2581 accacctgcc agggctcaag aagcggtggc ggcacgttcc ggcggcgcgg atgccgcgga
     2641 tcacgcacat cgaggccgac ggacaggtgc gcgaggtgga cgaggcgttc gaggtaggcg
     2701 gggaggaact gatgtatccg cgcgacccgg ccggctcgcc ggagaacacg atcaactgcc
     2761 actgcctgac ggagccctac ttcgagccgg aggcgctcga gccgacggaa aagcaacgcg
     2821 ggttgctgag cgatttgggg atcgaaatct ctgtcgcgcc cagataggcg cgggaaagga
     2881 tcatcatggc cgacgaggcg aagatcgaca ttccggaagt gccgccagtt ccggggctgc
     2941 cggagcaggt gaaagccgcc tggcggaagg cgtacgcgga cgcgttcaag cgagcgcagg
     3001 tggaccacaa agacgacccg ctgatgcagc ggcaggtggc gctcggcgag gcgaaccgga
     3061 tcttccgcct ggacgctccg gtggactatg acgacgcgat ggacctggcg ccgtggcagg
     3121 tgatcaagcg cgaggcgcgc gagggcgagc tgaaggtcgt gacgatcgat gggcagaagt
     3181 tcagcttccc agtgccggca ccaaagccgg cggcggtcgc gggcccgacg aggggcggcg
     3241 agaagaagta ggggaagcga gcgacttccc ttaacgcgac ggtctgccgc cgcccgaggc
     3301 gcggctggag gggcggcggg ccaggaggca agcgatggcg aagacgaaga tggtggacgg
     3361 cagggaactc acggcgagcg acttcgcgtt cgtgggcaac cccgacgatc cggaaacgtg
     3421 gaagctgccg gtccacgacg agggtcacgc gcgcgcggcg ctgggacggt tcaaccagac
     3481 cgacatgccg gcggatgcga aggccgcgac ggcgcggaag atcgtggcgg cgtgcaagaa
     3541 gttcgggatc gacgccagcg ggttcatgga ggagcacaag gcgatgatgg cggcgagctc
     3601 gtggttcgcc gtgtcgcagg cggcgcaatt cctggcagag gagggtgcgg gcgccgagga
     3661 ggggtacgtc tggccggtgc agatcatcga ggcggggcgc gcgcaaggtt cactcgtaga
     3721 cggtgacgga aagtccgccg gtaagacgcc acacgtcttc actgaagagg tggtggcgca
     3781 attcgcggcc gcagcgaacg gggcgcggtt cggccggcgg catccggcgg cgcttgaatc
     3841 ggacgacgat ccggcccgga tcgccggctg gttctccgag ggtgtggtgg ccgggaaggc
     3901 ggcgcgggcg aaactgcacc tgttgaagaa cgagcaggcg ctgcgggata agctcctggc
     3961 ggcgaaggac gcagggcagc tcggcctgtt cggtctctcg gtgctggcgt atgtcgcctt
     4021 ccagcgcggc aaacacgacg acctggatgc gctggtctcg gagcggctcg ggaaactgat
     4081 cagcgtggac ctggtgaccg aagcaggcgc gggcggaaaa ttcctggtgg cggcatcgaa
     4141 gtcgctggcg gcggggctgc gcgagttggt cgagagcgac gaagtaaaga accaggccgc
     4201 cgcggatgcg caaacggcgg cggagacggc tgcactgaaa gcccaggaag cggccgcggc
     4261 ggctgccgca gcggcaaggg ccgccgaagc caatgcgaaa agagctttgc cggtcacgaa
     4321 ccggcaagag aacggcgcag ctccgactgc tcggggcggc cggaacatgg gaggagcgat
     4381 gaaggaacgc attttgaaag tgctcgagac gctccggaag ttcgactccg gccgcgccga
     4441 tgagctgagt gcggagttca atgcgctgcc ggaagagcag cacgtcgaat tcctagggaa
     4501 ggtgaaggag gcgctggact cggtggacga agcgcagcgg acgcggggcg ccgaggcggc
     4561 gacggcgctc gagaagcggc tagccaaggc catccgggag gacaaactca tcgaggccaa
     4621 acttccggcg gaggccgcga acctggtgcg cgagcacctg gacggactcg gccgggtcta
     4681 cacggaggcg gagatcgacg cggagatcaa gagggtgcgg acggccttcg cgtcgttcag
     4741 caacgtcggt agggtgacgg aaggctcggg aatcacggtc ggccgggact cgcgggagaa
     4801 ggtccaactc gcgatggacg cgatgctggg cgtgaaggag gcgcgggcgg acaagagcgt
     4861 ccgggccttc cgcgggatcc gcgaggccta cggttggatc accggcgacc acgaactgac
     4921 gttcggcaac ggagggttcg gccgcgtgtc agaagcgatc gcgacggctg atttcccgaa
     4981 catcctgctg aactcgctca cgaagaagct gctcgaggac tatacggagc taggcatggg
     5041 cggcctcgaa ttgctcatca gcccggcgga catcagcgac tacaagacgc aggaccgggt
     5101 gcggatgggc tacctgggcg acctcgcgac ggtggcggaa gcaggtgcct acctggaaat
     5161 cacgaagccg accgacgaga agatccaata cgccgtgggg aagcgcggcg gtttgctgac
     5221 gatctcggag gagacgatcc gcaacgacga cacggggaag atccgccagt tcccgaaccg
     5281 gctggcgcgg gcggcgcggc ggacgctgaa gcagttcatc accgacttct tcgtcaacaa
     5341 cccgaacttc gatccggaca ccttggcgtg gttcgtggcg gggcactcga acctggggtc
     5401 gacggctttc tccgacgtgg agctggatgc ccgcgagatc gcgctgatga agcagacgga
     5461 gaaagactcg acgaagcggc tgggtctgcc gctcgagtgg atcatgatcc cggtggacct
     5521 caagtcggcc gcctaccagg tgaacaacaa caacgatggg gccaactcgt ggtaccaccg
     5581 gttcgggatg aacgagacga agcccgagcg gatcatcgtg aacgaactgc tgacggacgc
     5641 gaacgactgg ttcgggggct gcctgccgca gacggcgccg ttcctcgaga ttgggttcct
     5701 cgatgggcgg caggagccgc agatcctgct ggcgaacgac ccgcagaccg gcctggcgtt
     5761 caccaacgat caggtggtct acaaggcgaa gttcgtcttc ggcggcgaca tcaccgacta
     5821 ccggccggtg ttcaagaacg tcgtgccgta atcccgcgag tgcgggccgg tggttgagcg
     5881 gaccgcggcg gaagtgacat cctgggcccc cggtccgact tcctcatcgg aagccggacc
     5941 ggggcgagct cctagaggag gaaaagggca atgccaaggg acagacaaca cgcgcggacc
     6001 tttgtggtgg cgcttccgat cggcgacggc gccttgacgg cggcgaccga caaagcaatc
     6061 tggccggtga ggaagccttg ccggctgctc gagtgcagtt tgtcgctgag agccaccggc
     6121 ggcacgagcg gcaacaccga cgtggacgtg aacaagaacg ggacgtcgat cctggcggcg
     6181 ccgggtttgc ggatcgcaca gggcgcgacg acgaagtacg tcaacacgaa acccacgggc
     6241 gcagcgggga tgcccggcgg cgtggcgctg aagccgggcg acgtcgtctc ggtggacgtg
     6301 gacgcgattc cgggggcggc ttcgaccggc ggactcgttc tgctgcactg cgaagcgacc
     6361 gacatctgag cggtcgccgg cggctgaacc gcgggaagta aagcatggcg aagagtctcg
     6421 atcaattccg gcaggaactc gcgctgcgcg tccaggacgc ggcggcgaag ctctcgcagg
     6481 aagcacggga tgccatgatc gcccaggcca tcctgcaacg gtactcgaag gaccggccgg
     6541 ccgagaaagt gagcgacgtg gacggcaacg gcacggcgag cctggcgacg cccgccggct
     6601 tcgaggatgg tttctcgatt gtgcggtccg tcgaatatcc gatcggaaag gtcccgccgg
     6661 cgctgctcga agacgagggc tggatgatgt accggacgcc gacggcgctg cagatcctgc
     6721 tgacgtcggc cagaccagcg gcgagcgaga aactgcgcgt cacctggacg gcgaggcaca
     6781 acgccgacgg gagtacggtc cccgaaccgg acttcgaggc ggtctgcgac tatgcggcgg
     6841 cgctggcatt cgaggcgctg gcggcgatcc acgcgcagac gggcgactcg acgctcatgg
     6901 ccgacgcagt gaactacaga acgaagagcc aggagtacct cgggctggca aaggcggcgc
     6961 gaaagcgata cttcgatcac atcggcgtga gcgaggaccg ggcgggctcg ccggcgggcg
     7021 ggccggcgat gagcatcggc gacctcgacc aactgatggg ctccggggtg gaccggatga
     7081 cgcatccgaa gggcagccga tgagcccggt gatccgcatc acggtcaagg gcggcgagga
     7141 ggcggtcgcg gcgatccaga ctgcggtgcg cgagggcgtg accggaggac ttgaggcggc
     7201 gggattgcgc ggcgagcaac tggtcaagga aagcattctg gcgccgccgt tcggcccgcc
     7261 ggtggtggca acgggaacgc tggtgaactc aatcacccac cagctcgagg cgaacgatgt
     7321 cctggcggcg cgcgaggtcg tgttcgcgca ggcgccggcg gatcaatacg cggcctacgt
     7381 cgaggcgggc accgggccgc actttccgcc gccgcgggcg ctgctgctct gggtaaagaa
     7441 aaaattcggg atccgcacgg agaaggaggc gctgtcggcg gcgttcgctg tggccagggc
     7501 gatcgcgcgg cgcgggacgc tggcgcgcgg agtgttccac gcggccttcg ggaacttgac
     7561 ggcggagctc ggtggaatct tcagccgggc gatcggcgcg gcgctcgaga aggccggggc
     7621 agcaggcaga tagatggcgc tgaaagacat catcgcggag gttgccgcaa tcctagcgac
     7681 ggtgccggac atcgggaggg tgcatgccta ccggcgggcg gtcgcggcac agacggacat
     7741 tgaggcggtg ttcaaggatg tagtgaccgg acagatccgg gggtgggaag tgagccgcga
     7801 ggcgacagcg tcgcaggaca tgctggtggc gggaacccgc gagcggcatc agatcgtcgt
     7861 atacgggtat atgtcgatca acgacgcgaa gcgatcggag gaaacgttcc aggagctggt
     7921 cgaggcggtg cgggatgcgt tccgcgtcaa acgcaacctg aacggcaaag cgttcgacac
     7981 gacgccgatg agcgcgcgga ccgtgactgc ggcgatgaaa gcaggcgtac tcgtgcacta
     8041 ctgcgaactg acgatggagg cgcaacagtt tccggtaacg acggcgtgag gaagagatga
     8101 cggcggagga cttcgcggac gtggggttga cggaggcggg cgcggcctag ggagcggaga
     8161 gatggcgaga gacgactacg tcgagatcag gctgacgaag aagggcgccg aagcagccgg
     8221 cgtcgggacc gtgcggatcg tgaatgcact cgtggatgtg gaggtccgcg caggcgagac
     8281 tcaccgagtg agccgcgcag aatggaccga gattttcagt ttggcgcggc ttgatggcga
     8341 accgctgttc gagatcacgg ccgcggccga gccggcgaaa ccggcgatgc gacagaaagc
     8401 ggagaggagt tagacgatgg cgacctttgc ggcacaacaa atcggggcgc gggaaatcgt
     8461 gatcagcccg aacaagcaga acgcgtacgg cacagtgctg ggcgacgtga gtctggtgca
     8521 caacctgcgg ttcgacggcg gagcgttcgc cgatctggcg aaagagtaca tgacggacat
     8581 ggacctggcg aaggggcacc cctgggcgac gttcaagaac cctatccggc agaacatgcg
     8641 gttgtcgctc ggcggtttgc aagtctacga tttccttgct ggatggctcg cggcgttcct
     8701 gttcggcaag gtgcaaacca gcggcgtggg gccttacacg catctgttca ccgcggacct
     8761 ttcgaccacg attgccccgg tgacgacggc gtggatccac gataccgcgg acgtgaagtc
     8821 caaggtgccg gacctggcga tcaactcgct gcggttcaac ggcgcggcga tcgggccggt
     8881 cacggccgat gcggagctgg tcgggtcggg ccgcttcacc gacatcgcga tggcggcacc
     8941 ggccctggcg gttccgacgt acctcctgaa cagcgacgcg gacatcctgc tggggccgca
     9001 gggcgcgccg gtctcgatca aggagcgggt gcggagctgg tcagtgagcc tgacggcgaa
     9061 cctcgaggac taccggcacc cgggcagcgg actgtatgcg ggattcacgc ggcgaggcga
     9121 cctgcggatc accgtgcagc tcgggattgc ggcgaaggac gtggacgaca tccggacgct
     9181 cggcgtgctc gacacgaaac gcgaattgca gatcaacgta aattcgggcg cggccgcgca
     9241 gctcaagttc aagtttccgg cggtgatcct gcgggtgacc ggaggtgccg aggggacgtt
     9301 cgtcctgtgg accatcgagg cggatgacac cggcgtgctg aaagacggcg tgaacgaacc
     9361 gatcgaagtc acggcaatca acggccaggc aacgtacctg gttggagcat agttaccgcg
     9421 ccttccaaag cgccggcaac ccgggccttt ggaaggcgaa ccccggcacg ggcgcttcct
     9481 cagcgcaacg ccaatttcgg ccggggaaga ggacgcacac tgcgtccgaa ctcagggctg
     9541 ctgagcttga ccacagcggg cacggcaaca ccacgcacgg ccccgagcct gagagacctg
     9601 aaaacttttg tgggggtctc tcatggaaga agccctggca acgacagttg ggatcccaac
     9661 cgaaaggaaa gcagaggcgc tgctgccgct ggacctggcg gaacgcgcgg tgacctggtc
     9721 ggcgaacggg cgtgtgcgga cgcacgtctt tcgccggctg acagcggcgg attgggagga
     9781 ctatatcgcg cacatccggt tcgaggttga gacgggcgcg ccgccaagaa tcgacgtgac
     9841 tgtggccggg ctgcgcctgt acgaacgtgc agtgctcctg gcggaggggt atccggtacg
     9901 cggcgacgca aaactggaaa gcctgccgcg gtggaaggac ctgcttccca tcggggagcg
     9961 cctgcaggcg gtgcggctgc tgatgcaggt ggatcggcat gagggcgatg acggggtgat
    10021 gatcgagccc gacatccaga tcgcggccct cgatgctatc tggggcatca ccgggccggg
    10081 aagaacgcag acctatcgcg gcctgatcca ccggttcaca ccaccgaccg gagagcatct
    10141 ggcccggtac agccgcgaaa tcagccgggc gaagatcatc gggggcagtc gaaccggcct
    10201 caccgtgtac cagccgcggg aaaaaatcct ggtaaagctc tacgacgaat tgatccagag
    10261 cgtcgaaggc tactcggtca gaggcgagcc gttgaccgat cgcgaggcga ctgtgcggga
    10321 gatggaccca ttccacaagg cggcagcggc cagcattatt ttcgacctgc tggggcgacg
    10381 ggacgaggag gaaccggaag acaggccatg atcgaggtct ggcgagatcc ggcaggattg
    10441 cgtacggcga tggccgagat gctggagaac tcgctgcgcg cgcgcgtgcg gcgaagtttg
    10501 gcgcagcgga tgaagggcgc gacgaatgcg gaccgcgcgc tggctggact cgaggaccac
    10561 cggacgctgg cggacggata ctacgcctgg gccggccatc tggtgttgct ggaccgggtg
    10621 gtgcggcaag cggcgccgcg gaccgcgttg cgcatcgagg agctgcgcgg cctggcggac
    10681 ctgggcggag ttgaggctga gttcgagcga gcgcatccgc ggtgcccgcg atgcggcgag
    10741 atgcagacgg acgcggcaga actgcgctgc tggaactgcg ggaaggaatt cggggccccg
    10801 aggtagacga tgccgacgac agtggaaatc cagatcgtgg tggaccagga gggcgcgagc
    10861 cacgccttcc agcgggtcgg caacgaattc aaggagttgg aaaagtcggc gggcaaggtt
    10921 ggcggaacga gcaacgccgt attcacgcag atgcggaagc agcaggaaca ggcgcgcgac
    10981 gccgcgcaac tactatcgcg ggcagtcggg gcggaggtgc cccgggcgct ggagaaagtg
    11041 ctggcgcgga gcaataccgt aggcccggca ctggctacgg cattcaatgc ggctgtggtg
    11101 actagtttcg gagttgcggt gattgcggcg gttgacaaac tcttcgggtt cagtgagggg
    11161 cttgctcgga cgcgccgcca ggcagatgaa ctgttcgccg ctgtcgcgaa ggccaacgaa
    11221 attctggccg gtccaaagac tttgcaaggc gtcaatgcgc aaattcgcgc gaccgagtcc
    11281 agcatcaacg ccgcacaggc cacgatcgaa gtgctgaaac agatactcgg cggagggctt
    11341 aggggcgaac tgacagcccg gggccttcag acgctcggca tcttggagga cctcaacggg
    11401 gaactggata agcagaagaa gttgctgaac gagcagtacg tctcacaggc tcaactgatg
    11461 gatcagcaga tgcgtgagga gccggtggaa gtcttaaaac gagagaacga ggcgcggctc
    11521 gccggcatgg tggggattgg caagattcgc gccgaggaag ccatgacgct ggacctgctg
    11581 gagaagcaac taaaagcccg catcatcacg cttcccatct atcaggccga agtgaatgcc
    11641 gcccatgcga aagcgaaggc ggagttggag gcataccagg acctccaaag aggaatgacg
    11701 gctgcggcag tggccgcacg gaacgcggcg gtgctgcgcg agcggatgcc tgtggccgaa
    11761 ttgccgccag ccatcacgga gttgccgaaa tggatgcaag agatggccga ccagctcgac
    11821 aaggagtggg cgagcgagaa ggaaatccgc gggcggcaga tggagttggc cggcgaactc
    11881 gcggatgcag agagccgcgc ggcgatcgca tcgttggaac cctggcagaa ggcatacgcg
    11941 cagatccagg gggatgccga gcgggcgacg cgagaaatcg cgcagaggta cggaggcctg
    12001 tcagaggagg cggacaaggc agcgcggcta accaccgcgg tgtgggcgga ggcggggctg
    12061 aggatgcgcg accagctcgc cggagacctc gagagcgcgt tcgacgacat cacgagcggg
    12121 aacatcggga agaggttcct cgagaacttc aagaagatgg tcttccagat ggtcgcgacg
    12181 tggatgctcg gaatgcagca gatgcgcacg gcgtcgggtt tctcgttcgg ctcttttttg
    12241 agcggtttgt tcggcttctc gctgccaggc gcgggaggcg cggctacggt cggcgcagcc
    12301 gctgccggcg cggcgccggc tttctcgtgg acggccgaaa atgcccttgc gacatcgggc
    12361 gctttgccgc tgaatctcgg cggacgcgga acaagtggag tttcactttc gatggcggcc
    12421 ctgccgctct cggcggggca gggagcgggc gcaggcatcg gcgccgctct tccagcgggg
    12481 gccgcagtgg tcggcgcagc tggcgccgcg aaaacctcgt ggcttgcggc attgggcggg
    12541 ccggcaggac tggctgccat gggaggcatg ttcgggatcg gcctgctcgg caaccgttcg
    12601 accctcgcgc gcggaatcgg cggtgcgctg acaggcgcca cagcgggttt gtttggcggc
    12661 gctgtcctgg cttccctcgc actcgggatc ccactgtccg ccggtcttgc cgtttcgctg
    12721 gcggctgcgc ctttcacctt gggcctcagc gcactcgtcg gcggcctcct cgcggtgttc
    12781 ggattcggcg ggccacacaa gggcgacacc gcgcggatcc aggtgatgga gccggcgcaa
    12841 cggcagatca agaacatcct ggacgagtac aacctatttc agacgacgct cgaccaggcc
    12901 atctcgcagc tcgagcagct ccgctcgcag gggatcgagc ggctcagcca aatcggcggc
    12961 aggcagaaga gcagcaattc ggcgacgctg acgcgctggg tggacgaagc cgagaaccag
    13021 atgaagagca ccgaggcaga gcggcaacga agggttgctt tgcaattcgg cggagcgatg
    13081 ttccacagcg gcgggtacgt gaacccatca ctcgcgcggc agacggtgcc ggcgtgggcg
    13141 gcgggcgcgc cgagatacca atcgggtggg gctgtgccgg cgctgctcga gccgggggaa
    13201 tacgtggtgc gatcgcaggc cgtccagcgg atcgggcgcg gtaggctcga acagatgaac
    13261 gccggcggcg agggcggcgg gatcacgatc cacatcaacg cgctcgatgc gaggagcttt
    13321 tcggcgtggt tgcgcgacgg cgggatgaag gaaatggtgc gcgagctgcg tcgggccagg
    13381 gctgagggag cgttctagat gagcgagcag aacatcctga acccgagcgc aacgagtctg
    13441 ctgaactatg actggggcta cagcgaagga ctgccggaac tgcggtcggt ctggcaggca
    13501 cagtcgggca agatttattc gcggctgcag atggcgcgcg gacgggtcta cgacctcacc
    13561 tggaacaacc gcgacctggc gaccatgcac gcgctcaggc agtgggaaga gcagtaccgc
    13621 aacgacttct tcacgttagc cgactgggag cgcgggcggt atttcagcgg gcggttcgag
    13681 gggccgctgg cgttcaatcc ggcgggcttc gagaagtgga acatccgcgg gcgcttcgtg
    13741 gagctgccag gactggcgat gtacgcctat ccgagcaact gggcgcgcga tgcgattttc
    13801 tacgaggaac gcgacgggtt cggcgcggat ttggtaaaac tcgcgggaac atggatcttt
    13861 tccagctcac cgggggtctt ccacggggac gctgccggcc gatattactc agtcacgacg
    13921 agtgacacag cggagtggat tttcctgggt tacggatggc gggtctgggc caacactgag
    13981 aacgacgccg gcatcatgga gatgaaggtc accaaagtgc gcgatggctc ggtagtggcc
    14041 aatgccaaca tcgacctcta cagcgccgca acccagccgg cttcagcgtt gtggacggga
    14101 gtcggcattc cgctggatct ttatcgtgtc aggttgcgga tcaccggcac gaagaacccg
    14161 ctctcctcca actactgtgg cttcgcggac gccgttgagg tccttcgctg atgcggacgc
    14221 tgccggcggg act
//
LOCUS       DBNGWZ010000477         3723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_944527, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000477 DBNGWZ010000000
VERSION     DBNGWZ010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_944527"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="AC16CX_14760"
     CDS             <1..532
                     /locus_tag="AC16CX_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3013804.1"
                     /translation="DISEQDAHVAGFILEAGRALVVAVNKWDRLPQQERELVKRALVR
                     KLDFLGFARLHFISALAGQGLAPLMRSVDEAYAAAMARLPTPRLTRALYAAVAQQAPP
                     RAAYIRPKLRYAHQGGVNPPRIVVHGNALEHVPETYRRYLERFFRDAFKLQGTPLRVD
                     FKSAPNPYAERARNRR"
     gene            603..842
                     /gene="hfq"
                     /locus_tag="AC16CX_14765"
     CDS             603..842
                     /gene="hfq"
                     /locus_tag="AC16CX_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR3013805.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHIPVSIYLVNGIKLQGQIDSFDQYV
                     VLLKNTVTQMVYKHAISTVVPARPVNLSLDQSSDS"
     gene            864..1991
                     /gene="hflX"
                     /locus_tag="AC16CX_14770"
     CDS             864..1991
                     /gene="hflX"
                     /locus_tag="AC16CX_14770"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HIR3013806.1"
                     /translation="MFDRPGNGTEAVLVGLDFGEAGYTDSLEEIRQLAASAGVIARAM
                     VRGRRARPDPALFAGKGKVEEVARTLATTGARLAIFNHELSPVQERNLERELGCRVVD
                     RVSLILDIFAQRARSHEGKLQVELAQLEHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ESDRRLIGKRVKVLKEKLARVRSQREVQRRARSRAHLISVSLVGYTNAGKSTLFNRLT
                     RAGAHAADQLFATLDTTTRRVHTANGARLVLSDTVGFIRRLPHTLVAAFRATLEETVH
                     ADLLLHVVDAASEDREGQIAAVNQVLADIGAHAIPQMLVMNKIDRTPLAARVERDEYG
                     RISRIWASAQSGEGVEFIRLALEEHAAAAQAPAEYKSPAAA"
     gene            2079..3245
                     /gene="hflK"
                     /locus_tag="AC16CX_14775"
     CDS             2079..3245
                     /gene="hflK"
                     /locus_tag="AC16CX_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR3013807.1"
                     /translation="MSLNDPQWGRRPGQGPPDLDEIWRDFNRKLGGLFGRRGGGGGDE
                     PQGPDAKRVGGGAGLLLGLIFAVWLASGFYIVYEGQRGVVLRFGKYLELTMPGPRWHL
                     PYPIETAEVVNVAGVRSVEVGYRNNVKSKVLKESLMLTDDENIVDVQFAVQYILKSPT
                     EYLFRNRAPDEAVLQAAETAIREIVGKSSMDFVIFEGRAEVAAHAHKLMQEILDRYGT
                     GISISKVTMQNAQPPEQVQAAFDDAVKAGQDRERQKNEGQAYANDVIPKARGMAARLT
                     QEAEGYRQRVIEQAEGDAARFRQIVTEYNKSPQVTRDRLYIEAMQQIMTNTTKVLIDQ
                     KGGSNLIYLPLDKIMQMTGTGPAEAAPKPESAPAPEPSTARSREAFRSRERESR"
     gene            3245..>3723
                     /gene="hflC"
                     /locus_tag="AC16CX_14780"
     CDS             3245..>3723
                     /gene="hflC"
                     /locus_tag="AC16CX_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR3013808.1"
                     /translation="MKSNLGILLVGVLVALIVSSLSLFVVDQRQNAIVFRLGEVVNIK
                     KEPGLYFKIPLLDNVRNFDVRILTIDTAEPERFLTSEKKNVLVDLFVKWRITDVRQYY
                     VSVQGNESLAQTRLLQTINDGMRAEFGNRTVHEVVSGERDKIMDLMREKANEDAKTIG
                     "
BASE COUNT          726 a         1201 c         1231 g          565 t
ORIGIN      
        1 ggacatctcg gagcaggacg cacacgtcgc cggtttcata ctcgaagcgg gacgggcgct
       61 ggtggtggcg gtcaacaaat gggaccggct tccccagcag gagcgcgagc tcgtgaagcg
      121 cgcgctcgtg cgcaagctcg attttctggg atttgcacgg ctgcatttca tctcggcgct
      181 cgccgggcag ggcctggcgc cgctcatgag gtcggtggac gaggcctatg cggcggcgat
      241 ggcgcgcctg ccgacaccgc gattgacccg agcgctgtac gcggcggtcg cgcagcaggc
      301 gccgccccgg gccgcgtaca tccgccccaa gctgcgctac gcgcaccagg ggggcgtcaa
      361 cccgccccgt atcgtagtcc acggcaacgc gctcgagcac gtgccggaaa cctaccgtcg
      421 ctacctggaa cgatttttcc gcgacgcttt caaattgcag ggcaccccgt tgcgagtgga
      481 tttcaagtcc gctcccaacc cgtatgcgga gcgcgccaga aaccgcaggt aaccctatgg
      541 cgtaacagta aaaaaacgag taaagtagaa accttaaaaa cactacaaaa gcgaggctcg
      601 acatgagcaa caaagggcag ctgctacaag accccttcct caacaccctg cgcaaggaac
      661 acatcccggt gtcgatctac ctggtgaacg gcatcaagtt gcagggccag atcgattcct
      721 ttgaccagta cgtggtcctg ctgaagaaca cggtgaccca gatggtctac aagcacgcca
      781 tctcgaccgt ggtgccggcg cgccccgtca atctttcgct cgaccaaagc tccgacagtt
      841 gagcggtatt tatcttccga tcgatgtttg accgtcccgg caacggcacg gaggcggtgc
      901 tggtcggcct tgacttcggc gaggccggct acacggacag cctggaggaa atccggcagc
      961 tcgccgcgag cgccggcgtc atcgcccgcg ccatggtccg gggccggcgc gcccgccccg
     1021 acccggcgct cttcgccggc aagggcaagg tggaggaggt ggcccggacg ctcgccacca
     1081 ccggcgcgcg cctcgcgatc ttcaaccacg agctctcgcc ggtgcaggaa cgcaaccttg
     1141 agcgcgagct cggctgccgg gtagtggacc gggtcagcct gatcctggac atcttcgcgc
     1201 aacgtgcccg cagccacgag ggcaagctcc aggtcgagct ggcccagctc gagcacctcg
     1261 cgacccggct ggtgcggggc tggacccacc tcgagcggca aaagggcggt atcggcctgc
     1321 gcgggccggg cgagactcag ctcgaaagcg accggcggct catcggcaag cgggtgaagg
     1381 tgctcaagga gaagctcgcc agggtcaggt cgcagcgcga ggtgcaacgg cgcgcacgca
     1441 gccgcgcgca cctgatttcg gtgtcgctgg tgggctacac caacgcaggc aagtccacgc
     1501 tgttcaaccg cctgacccga gccggcgccc acgccgcgga ccagttgttc gccacgctcg
     1561 ataccacgac ccgccgggtg cataccgcca acggcgcccg cctcgtgctc tcggacaccg
     1621 tcggattcat ccggcggttg ccccataccc tggtcgccgc attccgcgcc acgctcgaag
     1681 aaaccgttca tgccgatctg ctgctgcacg tggtggatgc ggcgagcgag gaccgcgagg
     1741 ggcaaatcgc ggcggtgaac caggttctgg ccgatatcgg cgcgcacgcg atcccgcaga
     1801 tgctggtgat gaacaagatc gaccgcacgc cgctcgcagc gcgggtcgag cgtgatgaat
     1861 atggtagaat ttcacgcatc tgggcgagtg cacaatcggg cgagggcgtc gagttcatcc
     1921 gcctcgcact cgaggaacac gccgccgctg cgcaggcacc cgcagaatac aaaagccccg
     1981 ccgcagcgta acacgcatcc atatccacga tgcccggcaa cttgagtact ttccgcgact
     2041 atttgaggga ccgcttggcg cgcctgctcg gcgcattgat gtcgctgaat gatccgcaat
     2101 gggggcggcg tcccggtcag gggccgcccg atctggacga gatctggcgc gatttcaacc
     2161 ggaaactggg cgggctgttc ggccgccgcg gcggcggtgg cggcgacgag ccccagggtc
     2221 cggatgcgaa gagggtcggc ggcggggccg ggctgctcct tggcctcatt ttcgcggtgt
     2281 ggctggcaag cggcttctac atcgtctacg aaggccagcg cggcgtggtg ctgcgcttcg
     2341 gcaagtatct cgagctcacc atgccgggcc cgcgctggca cctgccgtac ccgatcgaaa
     2401 ccgccgaggt ggtgaacgtg gcgggcgtgc gcagcgtgga ggtgggctac cgcaacaacg
     2461 tcaagagcaa ggtgctcaag gaatcgctca tgctgaccga cgacgagaac atcgtggatg
     2521 tccagttcgc ggtccagtac atcctgaaga gccccaccga atatctgttc aggaaccgcg
     2581 cgcccgatga ggccgtgctg caggcggcgg aaaccgcgat ccgcgagatc gtcggcaaga
     2641 gcagcatgga tttcgtgatt ttcgaaggcc gggccgaggt cgcggcgcac gcccacaagc
     2701 tgatgcagga gatcctcgac cgctacggca ccggcatcag catcagcaag gtgacgatgc
     2761 agaacgccca gccgccggag caggtgcagg cggcgttcga cgacgcggtc aaggccgggc
     2821 aggaccgcga gcgccagaaa aacgagggtc aggcctacgc caacgatgtc atacccaagg
     2881 cgcgcggcat ggcggcgcgg ctcacgcagg aggccgaagg ctatcgccag cgcgtgatcg
     2941 agcaggccga gggcgacgcc gcgcgcttcc gccagatcgt gaccgagtac aacaagtcgc
     3001 cgcaggtcac ccgcgaccgg ctctacatcg aggcgatgca gcagatcatg accaacacca
     3061 ccaaggtgtt gatcgaccag aagggcggca gcaacctgat ctacctgccg cttgacaaga
     3121 tcatgcagat gacgggcacg gggccggccg aggccgcgcc caagcccgag agcgcgcccg
     3181 cgccggagcc ctccaccgcg cgctcgcgcg aagcattccg ctcgcgcgag cgggagtccc
     3241 gctgatgaaa agcaatctcg gcattctgct ggtgggcgtg ctggtcgcgc tgatcgtaag
     3301 ctcgctctcg ctgttcgtgg tggaccagcg ccagaacgcg atcgtgttcc ggctgggcga
     3361 ggtggtgaac atcaagaagg agccagggct ctacttcaag attccgctgc tcgacaacgt
     3421 gcgcaatttc gacgtgcgca tcctcacgat agacaccgcc gagccggagc gctttctcac
     3481 gtccgagaag aagaacgtcc tggtggatct cttcgtgaag tggcgcatca cggacgtgcg
     3541 gcagtactac gtgagcgtgc agggcaacga atcgctggcg cagactcggc tgctgcaaac
     3601 gatcaacgat ggcatgcgcg cggagttcgg caaccgcacc gtgcacgagg tggtgtctgg
     3661 cgagcgcgac aagatcatgg acctgatgcg cgagaaggcg aacgaagacg cgaagacgat
     3721 cgg
//
LOCUS       DBNGWZ010000478         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_690258, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000478 DBNGWZ010000000
VERSION     DBNGWZ010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_690258"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="AC16CX_14785"
     CDS             complement(<1..1083)
                     /locus_tag="AC16CX_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3013809.1"
                     /translation="MAGPLSHIRVLDLSRVLAGPWCGQNMADLGAEVIKIERPGTGDD
                     SRAFGPPWLKDWGGRDTKESAYFASANRGKKSVTVNLSKLEGQKIVRELARQTDVLIE
                     NYKVGDLARYGLGYDDLKKTNSRLIYCSVTGFGQTGPYRERPGYDFMIQGMGGVMSIT
                     GERDELPGGGPQRVGIPIADIMTGMYATIAICAALAHREKSGAGQHLDLALLDIQVGI
                     LANQGMNYLATGEVPGRIGNAHPNIVPYQPFKTKDGDVILACGNDNLFNKFCEVAGCR
                     HLAQDPRFATNSKRVENREEITKLLAAIFSKRTTREWCDALEAAGVPNGPINDLRQVF
                     EEPQVIARGMKIELEHPLAGKLPLIAS"
     gene            complement(1199..1996)
                     /locus_tag="AC16CX_14790"
     CDS             complement(1199..1996)
                     /locus_tag="AC16CX_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3013810.1"
                     /translation="MSSDGKPRIAVERDGAIATVVLSNPEKLNALTVSMWQELRRAMD
                     TLSADDSLRCIVLRGAGREAFAAGADIAEFATARDNAEQGRRYHREYVHGALKAVGEC
                     RHPTVAMIHGPCVGGGLEIACMCDLRIAGESGRCGVPINRLGFSIAYDELAALLPLTG
                     RAVALEILVEGRVWDAKEALEKGLLTRVVPDARLEEEAYAAARRIAEGAPLVNRWHKR
                     FMRRLTPEPAPLTEAEIEESFAYFATEDYRIGMDAFAKKRRPRFRGK"
     gene            2083..>2460
                     /locus_tag="AC16CX_14795"
     CDS             2083..>2460
                     /locus_tag="AC16CX_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3013811.1"
                     /translation="MTAIETIRHDVRICMFDLYGTVVDMQDGLTRAVTPYLRAKGWTG
                     DPSRLVTWWRRTHFENSMIDALLQRAHIPYREIGRISLSYTLERAGIAHTQDEVRRLV
                     AEIERLKPFPDVVEALARLKRKYR"
BASE COUNT          400 a          819 c          777 g          464 t
ORIGIN      
        1 gctcgcgatg agcggcagct tccccgcaag cgggtgctcg agctcgattt tcatgccgcg
       61 cgcgatgacc tgcggctcct cgaacacctg cctgagatcg ttgatcggac cgttcggcac
      121 gccggcggct tctagcgcgt cgcaccactc gcgcgtggtg cgcttggaga agattgcggc
      181 gagcagcttg gtaatttcct cgcggttctc gacgcgcttc gaattggtgg cgaagcgcgg
      241 atcctgcgcg aggtgccggc agccggcgac ctcgcagaat ttgttgaaga ggttgtcgtt
      301 gccgcaggcg aggatgacgt ctccgtcctt cgtcttgaat ggctggtacg gcacgatgtt
      361 ggggtgcgcg ttgccgatgc ggcccggcac ctcgccggtc gcgaggtagt tcatgccttg
      421 gttcgcgaga atgccgacct ggatgtcgag cagcgcgagg tcaaggtgct gacccgcgcc
      481 gcttttctcg cggtgcgcaa gcgccgcgca aatcgcgatc gtcgcgtaca tcccggtcat
      541 gatgtcggcg atcgggatgc ccacgcgctg cgggccgccg ccgggcagct cgtcgcgctc
      601 gccggtgatg ctcatcaccc cgcccatgcc ctggatcatg aagtcgtagc cgggacgctc
      661 gcggtacggc ccggtctggc cgaagccagt aaccgagcag taaataagcc gcgagttggt
      721 cttcttgagg tcgtcgtagc caagcccgta gcgcgcgagg tcgcccacct tgtagttctc
      781 gatgagcaca tcggtctggc gcgcgagctc gcgcacgatt ttctgtccct cgagcttcga
      841 tagattgacg gtgaccgatt tcttgccgcg attggcggag gcgaagtaag ccgactcctt
      901 cgtgtccctg ccgccccagt ccttcagcca cggcgggccg aacgctcgcg agtcgtcgcc
      961 ggtgccgggg cgctcgatct tgatcacctc ggcgccgaga tcggccatgt tctgcccgca
     1021 ccacggcccc gcgagcacgc gcgagagatc cagcacacga atatgtgaca ggggtccggc
     1081 catgaattac ctctggtaaa gcgtgataag tgacaagtga taggtgatgg gttctgtgcg
     1141 aacagtccag cgcctctttt gctcttcact tatcacttat cacctatcac gcctttcttc
     1201 atttgccccg gaaccgaggc ctccgcttct tcgcgaaggc atccatgccg atgcggtaat
     1261 cctcggtggc gaagtaggcg aagctctcct cgatctcggc ctcggtgagc ggcgcaggct
     1321 cgggcgtgag gcgccgcatg aagcgcttgt gccagcggtt gaccagcggc gcgccctccg
     1381 cgatgcggcg cgcggcggcg taggcttctt cttcgagcct ggcatcgggc acgacccgcg
     1441 tcagcagtcc tttttcgagc gcttccttcg catcccacac gcggccctcc accaggatct
     1501 ccagcgccac cgcgcggccc gtgagcggca gcagcgccgc gagctcgtcg taggcgattg
     1561 aaaacccgag ccggttgatc ggcacgccgc agcgccccga ttcgcccgcg atccgcaggt
     1621 cgcacatgca ggcgatctcg aggccgccgc ccacgcacgg gccgtggatc atcgccaccg
     1681 tcggatggcg gcactcgccc accgccttga gcgcgccgtg gacgtactcg cggtgataac
     1741 gcctgccctg ctctgcgttg tcgcgagcgg tggcgaattc ggcgatgtcg gcgcctgccg
     1801 cgaacgcttc gcgtcccgcc ccgcgcagga cgatgcagcg cagcgagtcg tccgcggaca
     1861 acgtatccat cgcgcgcctg agctcctgcc acatggatac agtcagcgcg ttgagctttt
     1921 ccggattgct cagcacaacg gtcgcaatcg cgccgtcacg ctcgactgct atccgcggtt
     1981 tcccgtcact cgacactcga cacccgacac tttgtaaggg cgcgttattc tacaatgcgc
     2041 cgagcaaacg cccgcgccgg cgccctccac ggagaaggac ccatgaccgc gatcgagacg
     2101 atcagacacg acgtcaggat ttgcatgttc gacctctacg gcacggtggt ggacatgcaa
     2161 gacgggctta cccgggcggt gacgccttat ctcagggcca agggatggac gggcgatcct
     2221 tcacggctcg tgacctggtg gcgccgtacc cacttcgaga actcgatgat cgatgccttg
     2281 cttcaacgag cgcatattcc ataccgcgag atcgggcgca tctcgctgtc atacacgctg
     2341 gagcgcgccg gcattgcgca cacccaggac gaggtgcggc gcctcgtcgc cgaaatcgag
     2401 cgcctgaagc cctttccgga cgtcgtggaa gcgctcgcgc ggctcaagcg gaagtaccgc
//
LOCUS       DBNGWZ010000479         4036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1951035, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000479 DBNGWZ010000000
VERSION     DBNGWZ010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1951035"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2859)
                     /locus_tag="AC16CX_14800"
     CDS             complement(<1..2859)
                     /locus_tag="AC16CX_14800"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3013812.1"
                     /translation="MNPRDPRLAAAGSFLVVGKDVPRTDAVPKVTGAAQYVADLHFPG
                     MLHAAVLRSPHPHARIVSIDTGAAAALPGVKAVAIGADTAKRKWGAFRPDLYPLAMDK
                     VRYVGDEVAAVAAVDPETARAAVDKIAVQYEALPAVLSLDQALAPGAALVHDDAPGNV
                     AHQFGFERGDVEAGFKAADVIVEGTWESARQWHTALETIGCVARWDNGRVTMWCNTQT
                     PFLARGRYATALGMSESQVRVIQTEVGGGFGGKSGDDNASVICALLARKSGRPVKLIH
                     TREEEFLASHPRMPMRYWVRLGFRKDGRILAKEIKMWADNGAYTGKSQAILGAATVRH
                     DALYKYPAVRAHSMLIYTNLVPTGAFRGFGNPSADWAVEQAWDLAAEKLGMDVLDLLR
                     MNAVDPGDVSPHNHKITSCELKQCMDKAATLIRWKEKRKARKPNREINEPAREIKEAA
                     RGLGMGCSVHVNGRRSFGDWDGASAIVRVNEDGRATVITGEGEIGQGNLTVLRQIAAE
                     ELGLPYEDVDITRPDTDVQPHSLGALASRLTYVAGNAVKNAAAAAAKQLLDAAAEQMN
                     RPTSELTIINGEIGPRNGSETDFKAVGAVVRANIYKRGGQPVIGVGTFDNPSEFPDHS
                     RYGNESGAYNYAAQAAEVEVDPATGEVKLLEVAAVVDCGTVINPATAEGQVQGAVTQG
                     IGLAMIEYFDWWNGMPTDPQLKDYPIPSAAMMPKLHIAFADSYEPSGPFGAKGLGEIG
                     LDAIPAVIANAVADAVGVRVHELPITAEKIHRAMHPELYANEKPATAAAPKGGTWTRL
                     SAGKPSGARPFSPEFVFAKTVEEALQLLAAGDAALVAGGMSHALRRERTGYPQAKRLV
                     SIMRIPELNEFSIDERGTLRAGAAVRQQAFFEDARVKNNWQAIDDAMEAVGHTRIRRM
                     LTVGGSVGPLIGGFDLPLALLALNARVTLAGSKGHRTVT"
     gene            complement(2856..3380)
                     /locus_tag="AC16CX_14805"
     CDS             complement(2856..3380)
                     /locus_tag="AC16CX_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3013813.1"
                     /translation="MITEYRRGDEIAQVLIKFRINGDPVEVNLKSGDRLLVDVLRDDL
                     RLTGTKHGCGIGACGTCTVIMDGYPIASCLQLAAMCDGADIRTVEGLAGTTALHPVQQ
                     AFVNRSALQCGICIPGHVMAACALLEKNPDPTEEEIRASADSVYCRCTGYIRILEAYR
                     EAIALARKARGPQP"
     gene            complement(3696..>4036)
                     /locus_tag="AC16CX_14810"
     CDS             complement(3696..>4036)
                     /locus_tag="AC16CX_14810"
                     /inference="COORDINATES: protein motif:HMM:NF024806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013814.1"
                     /translation="KAIQEKDWNTAIQSLTAAARRDTRNADVQNLLGYAYRNAGQLEP
                     AFKHYQHALQLDPRHLGAHEYIGEAYLMVNNLAKAEEHLAALKNLCAMVCEERDDLST
                     KIEEYRANRK"
BASE COUNT          617 a         1299 c         1353 g          767 t
ORIGIN      
        1 cgtcacggtg cgatgtccct tcgatccagc caaagtgacg cgcgcgttga gagcgagcag
       61 ggcgagcggc agatcgaagc cgccgatcag cgggccgacg ctgccgccca ccgtgagcat
      121 gcggcggatc cgcgtatggc ccaccgcttc catcgcatcg tctatcgctt gccagttgtt
      181 cttgacgcgc gcatcctcga aaaacgcctg ttgtcgcacc gcagcgcctg ctcttagcgt
      241 accgcgttca tctatagaga actcgttcaa ttcagggatg cgcatgatcg acaccaggcg
      301 cttcgcctgc gggtagccgg tgcgctcgcg gcgcaacgcg tgcgacatac cacccgcgac
      361 aagcgccgca tcgcctgcgg cgaggagctg caacgcttcc tccacggtct tcgcgaaaac
      421 gaattccggg ctgaaggggc gcgccccgga aggcttgccg gcggagagcc gcgtccatgt
      481 gccgcccttg ggtgcggcgg cggtggcggg cttctcgttc gcatagagct cgggatgcat
      541 tgcgcggtgg attttctcgg cggtgatggg cagctcgtgg acgcgcacgc cgactgcatc
      601 ggcgacggcg ttcgcaatga cggcggggat ggcgtcgagc ccgatctcgc cgagcccttt
      661 cgcgccgaac gggccggacg gctcatagga atcggcgaac gcgatgtgga gcttgggcat
      721 catcgcggcg ctcgggatcg ggtagtcctt gagctgcgga tcggtcggca tgccgttcca
      781 ccagtcgaaa tactcgatca tcgcaagccc gatgccctgc gtcaccgcgc cttgcacctg
      841 gccctccgcg gttgcggggt tgatgaccgt gccgcaatcc accaccgcgg ccacttccag
      901 cagcttcacc tcgccggtgg cggggtccac ttcgacctcc gccgcctgcg ccgcatagtt
      961 ataagcgccc gactcgttgc cgtaacggct gtgatccggg aactcggagg gattgtcgaa
     1021 cgtccccacg ccgatgaccg gctgcccgcc gcgcttgtaa atattcgcgc gcaccaccgc
     1081 gccgaccgcc ttgaagtcgg tctccgaacc gttgcgcgga ccgatctcgc cgttgatgat
     1141 ggtgagttcc gaggtgggac ggttcatttg ttccgcggcc gcatccagga gttgcttcgc
     1201 ggctgcggct gcggcgttct tcactgcgtt gcccgctaca taggtgagcc ggctagcgag
     1261 cgcaccaaga gagtggggct gcacgtcggt atcgggccgg gtgatgtcca catcctcgta
     1321 cggcagcccc agctcttccg ccgcgatctg gcgcagaacc gtgagattgc cctggccgat
     1381 ctcgccctcg ccggtgatga cggtggcgcg gccgtcctcg ttgacgcgga cgatggccga
     1441 ggctccgtcc cagtcgccga agctgcggcg gccgttgacg tggacgctgc agcccatgcc
     1501 cagcccgcgt gcggcctcct tgatctcgcg tgcgggctcg ttaatctcgc ggttgggctt
     1561 ccgtgccttg cgtttttcct tccagcggat cagcgtggcc gccttgtcca tgcactgctt
     1621 cagctcgcag cttgtgatct tgtggttgtg cggcgagacg tcgcccggat cgactgcatt
     1681 cattcgcagc agatccagaa cgtccatgcc gagcttctcc gcggcgagat cccatgcctg
     1741 ctccaccgcc cagtccgcgg aaggattgcc gaagccccgg aacgcgccgg tcgggaccag
     1801 gttggtatag atgagcattg aatgggcgcg cacggcggga tacttgtaga gggcgtcgtg
     1861 gcgcacggtg gccgcgccga ggatcgcctg cgacttgccg gtgtaggcgc cgttgtcggc
     1921 ccacatcttg atttccttcg ccaggatgcg gccatccttg cggaatccca gccgcaccca
     1981 gtagcgcatc ggcatgcgcg ggtggctcgc gaggaattcc tcctcgcgag tgtggatgag
     2041 cttgacggga cggccgctct tgcgggcgag aagggcgcag atgacggagg cgttatcgtc
     2101 gccggacttg ccgccgaaac cgccgccgac ctcggtctgg atcacccgca cctggctctc
     2161 ggacatgccg agcgcggtgg cgtagcggcc gcgcgcgagg aaaggcgttt gcgtgttgca
     2221 ccacatcgtc acgcggccgt tgtcccaccg ggcgacgcag ccgatggtct cgagcgcggt
     2281 gtgccactgg cgcgcggatt cccaggtgcc ttcgacgatc acgtcggccg ccttgaaccc
     2341 cgcctcgacg tcgccgcgct cgaagccgaa ctggtgcgcg acgttgccgg gcgcgtcgtc
     2401 gtgcaccagc gctgcgcccg gcgcgagcgc ctgatcgagc gagagcacgg cgggcagcgc
     2461 ctcgtactgg accgcaatct tgtccaccgc cgcgcgcgct gtttccgggt ccaccgccgc
     2521 gaccgccgcg acctcgtcgc ccacgtagcg caccttgtcc atcgcgagcg ggtagaggtc
     2581 cgggcgaaag gcgccccact tgcgcttcgc cgtgtccgcg ccgatcgcga ccgctttcac
     2641 tcccggcagg gccgctgcgg cgccggtgtc gatggaaacg atccgggcgt gcgggtgagg
     2701 gcttctcagc acggccgcgt gcaacatgcc ggggaagtgc aggtcggcga cgtactgagc
     2761 cgcgccggtg actttcggca cggcgtcggt gcgtggaacg tccttgccca cgacgagaaa
     2821 gctgccggct gccgccagcc tcgggtcgcg cgggttcatg gttggggacc ccgggccttg
     2881 cgcgcgaggg cgatcgcttc ccggtaggct tcgaggatgc ggatatatcc cgtgcagcgg
     2941 caatagacgc tgtccgcgct ggcgcggatc tcctcttcgg tgggatcggg attcttctcg
     3001 agcagggcgc acgcggccat gacgtggccc ggtatgcaga tgccgcattg caaggcggac
     3061 ctgttcacga aggcctgctg cacggggtgc agtgcggtag tgcccgccag accctcgacc
     3121 gtgcggatgt cggcaccatc gcacatcgcg gcgagctgca ggcaggaagc gatggggtaa
     3181 ccgtccatta tcaccgtaca ggtgccgcag gcgccgatgc cgcagccgtg cttggtgccg
     3241 gtcaggcgca ggtcatcccg cagcacgtcc accagcaggc ggtcgccgga cttgagattc
     3301 acctcgaccg ggtcgccgtt gattcgaaac ttgatcagca cttgcgcgat ttcgtcccct
     3361 cgccggtatt ctgtgatcac cgcgcgttga acggcggtga ttccgtccgt tcgaatgcgc
     3421 ggagttaaat cggcggcctc cgtccgattt aacgggaatt atatggcatc cctgcatctg
     3481 cctcgcatcg accgtgagcg cgcaataaaa gaggctcgcg tagcgtaagc ctttttcagg
     3541 acgcgtgatg gcggttacag gagcagggaa agggcttctt gcagcttgtc gagcagatag
     3601 tagaacagga acgaggatga ataaacgata gccacgcggc cttcgtgctt attgaggcgg
     3661 gcgaacgtag aagagcagat gggagtctaa ggccgctatt tccggttggc gcggtactcc
     3721 tcgatcttcg tgctaaggtc atcgcgctct tcacaaacca tcgcgcacaa gttcttcagt
     3781 gctgcgaggt gttcttcggc cttggcgagg ttgttgacca tcagatacgc ttcgccgatg
     3841 tactcgtgtg caccgaggtg gcgcgggtcg agctgcagcg cgtgctggta gtgcttgaag
     3901 gcgggctcaa gctggcctgc gttgcgatac gcgtagccca gcaggttctg gacgtcggcg
     3961 ttgcgcgtgt cgcgccgcgc cgcggccgtg agtgattgga tcgcggtgtt ccagtctttc
     4021 tcctggatcg ctttcc
//
LOCUS       DBNGWZ010000480         5171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_817670, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000480 DBNGWZ010000000
VERSION     DBNGWZ010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_817670"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC16CX_14815"
     CDS             complement(<1..428)
                     /locus_tag="AC16CX_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR3013815.1"
                     /translation="MPELPEVETTRRGLEPLLVGQRIRSVVVRNPRLRRPVPRRLPQL
                     VAGAMIRALARRGKYLLVDCGTGTLILHLGMSGRLWVVRDGAAPTPHDHFDLVLDNGT
                     VVRLRDPRRFGLVLWQAGDPFAHALLADIGPEPLSAGFDGA"
     gene            complement(518..1375)
                     /locus_tag="AC16CX_14820"
     CDS             complement(518..1375)
                     /locus_tag="AC16CX_14820"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR3013816.1"
                     /translation="MSRASWLNALLAAVVAALGAWAYFKPAKDAPVEYPLSALKTSGA
                     KSIRIERANAAPVALENKQGAWFITAPFAARGDEVRVRRLLEILEARSPHRLAATDLG
                     RFDLEQPRARVAIDGQVFGFGMVSPVASEQYVLTNGAVFAVHPRYGAALPANAAELAS
                     RQLFGPSETPVRLELANFTVEQRDGKWRLNPNAGALSQDDLIRWVEEWRLASAVRVEP
                     HVKGKLLAEIRIQLKEGGALSLGLLAREPELVLLRSDEKLQYHFRAETAKRLLSPHGA
                     AGIEPAGKK"
     gene            complement(1372..2718)
                     /locus_tag="AC16CX_14825"
     CDS             complement(1372..2718)
                     /locus_tag="AC16CX_14825"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HIR3013817.1"
                     /translation="MEITRKRRLQMMVHHGLFVVLLVLAAALLAYLAREYRVEYDLTR
                     AHRNTLSAATLDVLKQLNGPVGVTAYAMARDARGEHLHRRIEEFLQPYQRAKPDITLT
                     LVDPREQPRAAAQAGVRAPVELVVEFKRRTERLTEFNEQAFANVLMRLARGAERLVMW
                     LDGHGERKLDGIANHDLGEFGRLLEHKGFRSGSLNLALAQEVPANAALLVIASPQVDL
                     LPSEVQKIRRHMAAGGNLLWLIDPEPLRGLAPIAEMLGLVLTPGVVADPDAARLNVSP
                     TVAVAAVYGRHAITDGFNLITVFPQARQINIIESDEWRIRPFVEVAPRGRVKTGKAEG
                     RCDRNRDIPGPITIAAAFERMVGDRQQRVVVVGNASFLSNTFIGNGGNRDLGVNIVNW
                     LAGDDNLITIQPRSATDSSIDIDQAALYLIAFSFLIVLPLTFVITGAVIWWRRRRI"
     gene            complement(2728..3498)
                     /locus_tag="AC16CX_14830"
     CDS             complement(2728..3498)
                     /locus_tag="AC16CX_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3013818.1"
                     /translation="MILTIAGKELKALFASPLAWVVLSVVQIIVGYAFLRRLDDFLQL
                     QPQLASMASAPGATELVAAPLFGTAAALLLFAVPVLAMRLIAEERRNQTMVLLMSAPV
                     SMTEIVVGKFLGLCAFLLGIVALTALMPLSIAGVTRLDYGLLAGLAAGLALLAAGFAA
                     VSLYVSCLTVHPVASAIGAFAALLGMMLAGEAAGDGLRARGWAVAAGLVQVLSPIKNF
                     EPFGKGMLDTYALACSVLLIAVFLALAVRQLDAQRLRG"
     gene            complement(3636..4580)
                     /locus_tag="AC16CX_14835"
     CDS             complement(3636..4580)
                     /locus_tag="AC16CX_14835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3013819.1"
                     /translation="MPEPDTAATISARRLTRSFGSHVAVNNIDLELRRGEVLGLLGPN
                     GAGKTTTMQMLTGNLAPSRGAIHICGIDLLEEPASAKARVGYLPEVPPLYRDLTVDEY
                     LTLAARLHRVPRARRRDAVAAARQRCGLADAGARLIGTLSKGYQQRVGIAQAIVHAPD
                     VVILDEPTIGLDPNQIREIRRLIRELGGAHSVILSTHILPEVEAVCDRVQIMHHGNIV
                     YSDTIGALKQFHSGATVLVELRRPPPLAELAALPGVAAAEAIEPTLIRIQFAAGEDPT
                     DALVKQSVARDWGLYQLKPAQTSLEDVFVNLTRREENH"
     gene            4907..>5171
                     /locus_tag="AC16CX_14840"
     CDS             4907..>5171
                     /locus_tag="AC16CX_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013820.1"
                     /translation="MIPRRFIVLLACMALAACAQQPAQPRPPGESSPAGETKPPRVVV
                     AKPEPRKPALPNVELSEELMLKMMLAEVAAQRGQPHIAVQAYLE"
BASE COUNT          861 a         1725 c         1743 g          842 t
ORIGIN      
        1 gcgccgtcga acccggccga gagcggctcc gggccgatgt ccgccagcag ggcgtgggca
       61 aacggatcgc ccgcctgcca cagcaccagg ccgaagcggc gcgggtcgcg cagtctcacc
      121 accgtcccgt tgtccagcac gagatcgaaa tggtcgtgcg gggtcggcgc cgcgccgtcg
      181 cgcaccaccc acagccgtcc cgacatgccg agatgcagga tcagagtgcc cgtgccgcaa
      241 tccaccagca ggtacttgcc gcgacgcgcg agcgcgcgaa tcatggcgcc ggcgacgagc
      301 tgtggcaggc gacgcggcac cggccggcgg agccgcgggt tgcgcaccac cacggaccgg
      361 atgcgctggc ccacgagcaa cggttccagg ccgcggcgag tggtctcgac ctcgggtaac
      421 tcgggcatat cagaaccggt gaccagtgat cggtgaccgg tgaccggtgg tcacggattg
      481 cactcgaacc tttgaatcac gattcacaaa tcgcgattca cttcttgccc gccggctcga
      541 tgccggcggc tccatgcgga gagagcagcc gcttcgcggt ctcggcacga aagtggtact
      601 gcagcttttc atcggagcgc agcaacacga gttccggctc gcgcgcgagc aggcccaatg
      661 aaagcgcgcc gccttccttc aattgaatcc ggatttctgc cagcagcttg cccttgacat
      721 ggggttcgac gcgcacggcg gacgcgagcc gccattcctc cacccagcgt atgaggtcgt
      781 cctggctgag cgctcccgca ttcggattga gcctccattt gccgtcgcgc tgctcgactg
      841 tgaaattcgc cagctcgagg cgcaccggag tctcgctggg gccgaacaac tggcggcttg
      901 cgagttcggc ggcgtttgcc ggcagcgccg ccccgtagcg cggatggacg gcgaacacgg
      961 caccgttcgt cagaacgtac tgctcgctcg ccaccgggct caccatgccg aagccgaaga
     1021 cctgcccgtc gatggcgacg cgtgcccgcg gttgctccag gtcgaagcgc ccgaggtcgg
     1081 tggccgccaa ccggtgaggg ctccttgctt ccaggatctc gagcagccgc cgcacccgca
     1141 cctcatcgcc gcgcgctgcg aacggggccg tgatgaacca ggcgccctgt ttattctcga
     1201 gcgcgacggg cgccgcgttt gcgcgctcga tgcggatgga tttcgctccc gaggtcttga
     1261 gcgcggacaa cgggtattcg acaggcgcgt ccttcgccgg cttgaagtag gcccatgcac
     1321 cgagcgcggc gaccacggcg gcgagcaacg cgttcaacca cgatgcccgg ctcatatcct
     1381 gcgtctgcgc caccagatca ccgcgccggt gatcacgaag gtgagtggca gcacgatcag
     1441 gaagctgaac gcgatgaggt agagggcggc ctggtcgatg tcgatgctgc tgtcggtggc
     1501 gctgcgcggc tggatcgtga tcaggttgtc gtcgcccgcc agccagttga cgatattgac
     1561 gccgagatca cgattgccgc cgttgccgat gaaagtattg gagaggaagc tcgcgttgcc
     1621 caccaccacc acgcgctgct gccggtcgcc gaccatgcgc tcgaaggcgg cggcaatcgt
     1681 gatcggcccg ggaatgtcgc ggttgcggtc gcagcgcccc tccgctttcc cggtcttgac
     1741 gcggccgcgc ggcgccactt cgacgaacgg ccggatgcgc cactcgtcgc tttcgatgat
     1801 gttgatctgg cgcgcctgcg ggaacaccgt gatcaggttg aagccgtcgg tgatcgcgtg
     1861 gcggccgtac acggcggcca ctgcgaccgt gggggaaacg ttcaggcgcg cggcgtcggg
     1921 atcagccacg actccgggcg tgagcacgag acccagcatc tccgcgatcg gcgccagccc
     1981 ccgcagcggc tcgggatcga tcagccacag gagattgccg cccgcggcca tgtggcggcg
     2041 gatcttctgc acttccgacg gcagcaggtc cacctgcggg ctggcgatca ccagcaacgc
     2101 ggcgttggcc ggtacttcct gggcaagcgc gaggttgagg ctgcccgaac ggaagccctt
     2161 gtgctcgagc aggcggccga attcgccgag atcgtggttg gcgatgccgt cgagcttcct
     2221 ctcgccgtgg ccgtcgagcc acattaccag ccgctcggcg ccgcgggcga gccgcatcag
     2281 cacgttggcg aacgcctgct cgttgaattc ggtgagacgc tcggtccggc gcttgaattc
     2341 caccaccagc tcgaccggcg cgcgcacccc ggcctgcgcc gcggccctgg gctgctcgcg
     2401 cgggtccacc agggtgagcg tgatatcggg cttcgcccgc tggtagggtt gcaggaactc
     2461 ctcgatccgg cggtgcaggt gctcgccgcg cgcgtcccgg gccatcgcgt aggccgtgac
     2521 gccgaccggg ccgttcaact gcttcagcac gtccagcgtg gcggcggaga gcgtgttacg
     2581 gtgggcgcgc gtgagatcgt attcgacgcg gtactcgcgg gcgagatagg cgagcaacgc
     2641 cgcggcgagg acgagcagca ccacgaacag cccgtggtgc accatcattt gcagacggcg
     2701 cttgcgggtg atctccatgg ccgccggcta ccctctcagc cgctgggcgt cgagctgccg
     2761 caccgccagc gcgaggaaca ccgcgatcag caagacggag cacgcaagcg cgtacgtgtc
     2821 cagcatgccc ttgccgaacg gctcgaagtt cttgatcggg gacagcacct gcaccagccc
     2881 ggcggcaacc gcccacccgc gcgcgcgcag gccgtcaccc gccgcttcac cggccagcat
     2941 catcccgagc agcgcggcaa aggcgccgat cgccgatgcg accgggtgca ccgtgagaca
     3001 ggacacgtag aggctcacgg cggcgaaacc ggcggcgagc agcgcgaggc cggccgcgag
     3061 ccccgcgagc agcccgtaat ccagccgggt caccccggcg atcgagagcg gcatcagcgc
     3121 ggtgagggcc acgattccca gcaggaacgc gcacagtccg aggaatttgc cgaccacgat
     3181 ctcggtcatg gagaccggcg ccgacatgag cagcaccatg gtctggttgc ggcgctcctc
     3241 cgcgatcagc cgcatcgcca ggaccggcac cgcgaacagc agcagcgccg ccgccgtgcc
     3301 gaacagcggc gccgccacca gctcggtggc gcccggcgca ctggccatgc tcgcgagctg
     3361 gggctgcagc tgcaggaaat cgtcgagccg gcgcagaaac gcgtagccga cgatgatctg
     3421 caccacggag agcaccaccc acgcgagcgg cgaggcgaac agcgccttca gctccttgcc
     3481 cgcgatcgta agaatcatga tctaaccgcc aagaacaacg gaatgaaatc ctcaaccgtg
     3541 agtgcgtgac ccatcaacag tatcctctat gcgcggatcg gcgcgagggc gtttgtcgca
     3601 atacatgttg ttgaatcctc ggcgttctgg gcggttcagt ggttttcctc acggcgggtg
     3661 aggttcacga acacgtcttc aagactggtc tgcgcgggtt tcagctgata gaggccccaa
     3721 tcgcgtgcca ccgactgttt caccagcgcg tcggtcggat cctcgcccgc ggcaaactgg
     3781 atccggatga gcgtcggctc gatcgcctcg gccgccgcca cgccgggcag ggccgcgagt
     3841 tcggcgagcg gcggcggacg gcgcagctct accagcacgg tcgcgccgct gtggaattgc
     3901 ttgagcgcac cgatggtgtc gctatagacg atgttgccgt ggtgcatgat ctgcacgcgg
     3961 tcgcacaccg cctccacttc gggcaggatg tgggtggaca ggatcacgct gtgcgcgccg
     4021 ccaagctcgc gtatcagtct tcggatctcc cggatctgat tggggtcgag accgatcgtg
     4081 ggctcgtcca gtatcacgac gtccggcgcg tgtacgatcg cctgggcgat gcccacgcgc
     4141 tgctggtagc ccttggacag ggtaccgatc agcctggctc cggcatcggc gaggccgcag
     4201 cgctgcctgg cggcggcgac ggcgtcgcgg cgccgcgcgc gcggcacccg gtggagtctg
     4261 gccgccagcg tgaggtattc gtccacggtc agatcgcggt acagcggcgg cacttccggc
     4321 agatagccga cgcgcgcctt ggcgctcgcc ggctcttcca acaggtcgat gccgcagatg
     4381 tggatcgcgc cgcgcgaggg ggcgaggttg cccgtcagca tctgcatggt ggtggtcttg
     4441 ccggcgccgt tcggcccgag gagaccgagc acctcgccgc gccgcaactc gaggtcgatg
     4501 ttgttgacgg cgacgtggct gccgaaactg cgtgtgagcc tcctcgcaga gatcgtggcg
     4561 gcggtgtcag gttccggcat cgtggcgatt tttaattgtg caaaccgcgc ttgtggagtg
     4621 cacgaaatat acctaatatg tggctgttta tgaagcctcc ggaccgcttc gcgcagcgtt
     4681 gatccatccc gcgtttcatc ccattaggag tttgtcgggc ttggccccga caaactcctg
     4741 agcaaaaccg gcaccaggaa aattgaaaag aggatgacag acatgaagat tcactccggt
     4801 ttttctttac atccggccgt ttcgggcggg tttatttccg aatggcccgc cggttttgcc
     4861 ttcagcagcc tgtatggact gtcaagtccg acaggctgct agtcccgtga tcccgcgtcg
     4921 cttcatcgtg ctgctggcgt gcatggcact cgccgcctgc gcgcagcagc cggcgcagcc
     4981 ccggccgcca ggggagtctt cgccagcggg ggaaaccaag ccgccgcgcg tggtggtggc
     5041 gaaaccggag ccgcgcaagc ccgcgttgcc caacgtcgag ctctccgagg agctgatgct
     5101 caagatgatg ctggcggagg tcgcggcgca gcgcgggcag ccgcacatcg cggtgcaggc
     5161 ctatctcgag c
//
LOCUS       DBNGWZ010000481        10730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2079214, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000481 DBNGWZ010000000
VERSION     DBNGWZ010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10730
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2079214"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..342
                     /locus_tag="AC16CX_14845"
     CDS             121..342
                     /locus_tag="AC16CX_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR3013821.1"
                     /translation="MKTAKVFRHGNSQAVRLPKEFRFDGDEVFVKRSSGGVLLLPKRI
                     TMDQLDAILSQFRGKFVRRQPRMQKRRWR"
     gene            339..749
                     /locus_tag="AC16CX_14850"
     CDS             339..749
                     /locus_tag="AC16CX_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3013822.1"
                     /translation="MIWMLDTDTVAYLVNRAPGHERIKRRLSGRSPGEIRISAITLGE
                     LYYGFELGDVSVERTQALKELLELFQTEDFPADAARAYGEIRAALERAGRRIGPYDLL
                     IASHAQHLGAVLVTNNEREFRRVPGLSVQNWLKA"
     gene            1023..1910
                     /locus_tag="AC16CX_14855"
                     /pseudo
     CDS             1023..1910
                     /locus_tag="AC16CX_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
     gene            2035..2784
                     /locus_tag="AC16CX_14860"
     CDS             2035..2784
                     /locus_tag="AC16CX_14860"
                     /inference="COORDINATES: protein motif:HMM:NF016442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HIR3013823.1"
                     /translation="MDRFEEIVQSGLRQAIRHYWLTRTKQIDGQQAGSVRDQGRRGEV
                     TGGKQLDGFLTVIRDVLALGGIKKPEVFTGQIDSYLPGYFRPTKRWDLLAIADGNLLA
                     CIEMKSQAGPSYGNNFNNRVEEAIGNAHDFWRAYQEHAFKTQTKPFLGYIMLLEHDEA
                     STKPVRENEPHFNVRPEFKNASYARRYELFCEKAFREGLYDATAFLMSEKDGGLDGAY
                     IEPSKDLSFKNLAAALYGRAVAYSKQIGRGS"
     gene            2833..3450
                     /locus_tag="AC16CX_14865"
     CDS             2833..3450
                     /locus_tag="AC16CX_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013824.1"
                     /translation="MLEEAINSLLGKKQTSTKRLTLLADHLKHMLEVAGLPNVQGGTG
                     GELRVPGLARSKDWDIAYEFAGKFRLLISLKSMLGNISGSVPNRLDDLQGEIANIQQL
                     RPEIVIGYVVLFDVAKDARRKDGTLWSDFFKSSIQKISIRRAPLWNQGLLEASWFIRF
                     NSKLPMGRRVVAPENVEKEGRYFISALLSELKLREPAIHFSKPIR"
     gene            complement(3614..5770)
                     /locus_tag="AC16CX_14870"
     CDS             complement(3614..5770)
                     /locus_tag="AC16CX_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HIR3013825.1"
                     /translation="MTSCRPNMRPVRTLACAFAAALLAAAPAQARITKIVIDETVSPA
                     FCKGGACASYGEAGQYEQIAGRAWGELDPADPLNGLIQDIALARDADGKVRYVATFVI
                     TRPVDIGKASGLMWHDVPNRGRPVLIDTSEREFGDIGLASAWQGDNASMAAALGTAVR
                     PAMAAGGNHWLQLPVAKNPDGSPITGPVFARIVNRSGAGAQPLIVQTNPVPYLPASLD
                     TATAALVSRDHETIGGKVTGERKIAPEDWKFCGGGTFEEPVPLAKLPVQICLKGGFDA
                     AKLYQVVYTAKDPYPLGIGFAAWRDVGSFFKYAAQDDFGTPNPISGRITHSIARGRSQ
                     SGNYLRGWLHLGFNQDEAKRQVHDGMWPIIAGRRIALNFRWAQPDGVLELYQAGSEGP
                     QWWGPYPDKARNLPTRSIQDRCTATRTCPKIIEHFGAAEIWALKLGPEWVGTDPKEDI
                     ALPDNVRRYYIASSPHGGGRGGFDTSLAETHKAAKPVACPGNNWGAGVLPANPLPHTE
                     TLNAIRVHFRSWVMRGTPPPPSRYPTMRAGELVAPHKAAMGFPTIPGLRPTAPEPDFI
                     NPLLDYDWGPRFDPNDASGVPANAPPKIRQALKMLAPKVNADGNEVGGVPVVLLDAPL
                     GTYLGWNITAGGARPFHQGKLCNYAGGMIPFAKTRAEREAANDPRPSLEERYGDHAGY
                     VAAVRNAAANAMAQGFLLQKDAEALIKAAEASKVLK"
     gene            complement(5934..7067)
                     /locus_tag="AC16CX_14875"
     CDS             complement(5934..7067)
                     /locus_tag="AC16CX_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3013826.1"
                     /translation="MADSSPVKIGLLLPTTGVDTAQGTDATKGFELYLGKIGYRAGGR
                     EIRLFKEDDEAKPAVALARLKKLVEDSRVDFLIGPISSAVALAIRNYIHDQGTPLLVP
                     AAFTRVLTSPQQASPSIFRLSETSDQANYSMGAWMMKHAPYRRMIVMAADFAGGRHSV
                     EAFMAAFRAGGGEIVAEIYPPFDTPDFSPYLARIGSLRADAAYAWFAGADAVNFVKQY
                     RESAIGRQLPLTGHSALTVETILPTIGDAALGIVAVGAYSAAIDTPENRQFVRDYEAA
                     FRAVPSRYSECGWDAAALIATALDSLNGDVSDRGRVRDALASALPRIKPPRGPMRFDA
                     YRQAITPIYITRTERQGGRIVNVVVDKIPDVSQAATWGWWNKS"
     gene            complement(7434..8054)
                     /locus_tag="AC16CX_14880"
     CDS             complement(7434..8054)
                     /locus_tag="AC16CX_14880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3013827.1"
                     /translation="MLTIARWHEMWKELGAANPDPKLHGELMARYSEPHRKYHTLQHL
                     DECFEKLDEVRALAEHPAEIELALWFHDAIYDVKRRDNEKKSADWARASALAAGIPAE
                     ACNRVHSLVIATRHKAVPRGTDAEILVDVDLSILGTAPERFDQYERQVREEYAWVPDF
                     LFRRERKAILKEFLARPQIFSSTHFRDRYEQQARSNIERSLERLRA"
     gene            complement(8342..10036)
                     /locus_tag="AC16CX_14885"
     CDS             complement(8342..10036)
                     /locus_tag="AC16CX_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3013828.1"
                     /translation="MNFSEDHDVIVIGAGAGGMTAACVAAAEGLATLLVEKADRVGGT
                     TSVSGGMVWVPANAKMRAAGLTDSLEQARLYLSHTVPGDFNRELREVFLTHAPEAIEY
                     LEARTALALKPVTFYPDYYPDLPGATLGGRVLEPVPFDGRELGRHFGLLRPPLPEFTM
                     FGGMMVDRADIPHFRKAWRSLPSALRVARVAARHARDRLSFDRGAHLVLGNALAARLL
                     KSVVTLDVKLRTSTQVASLAMEGGRVAGVTLADGTRLRARRGVVLAAGGFSNDAGLRA
                     RYLPAETGPSSAACPANSGDGLRLGLAAGGRMVEENTDNAYWAPVSRFHRADGTPAVY
                     PHSVTDRGKPGYIAVDRRGQRFTNEAVSYHEFVRAMFRAHAIPAFLICDRASLWKYGL
                     GAVKPFTAFPGAHTASGYLARGSSIRALAGALGIDAAALEATVLRYNEDARTGVDRAF
                     GKGSDAYGKYLGDADHAPNPCVAPLERAPFYAVALYPGDLGTAAGLRTNVHAQVLDAA
                     GAPIAGLYACGNDMNTIMNGGYPGPGITLGPALTFGYVAGRHLARAESPAKEAVHD"
     gene            complement(10469..10651)
                     /locus_tag="AC16CX_14890"
     CDS             complement(10469..10651)
                     /locus_tag="AC16CX_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013829.1"
                     /translation="MPKIDYAYETTEEKIRAYLRVPEIDRLRWLEELCAFTKMAREAP
                     TAYSPAQEKYGEDTKR"
BASE COUNT         1956 a         3256 c         3485 g         2033 t
ORIGIN      
        1 cgggcgattc gagccgcggg tcacggcggc ggccgcccgc cggcagaaac gcgcccgcgg
       61 cggattgagc gcgccggcgg tgaagtatat acttggcgta tctatctcac gagggatccg
      121 atgaagacgg ccaaggtgtt caggcacggc aacagccagg cggtgcggct gccgaaggag
      181 ttccggttcg acggcgacga agtcttcgtg aagcgctcct cgggaggcgt gcttctgctt
      241 cccaagcgga tcacgatgga tcagcttgac gcgattctgt cgcaattccg cggcaagttt
      301 gtacggcgcc agccccggat gcagaaaagg cgctggcgat gatctggatg ctcgataccg
      361 atacggttgc ttatcttgtc aaccgtgcgc ccggccatga gcggatcaag cgaaggctgt
      421 cggggcgttc cccaggtgag atccggattt cagcgatcac gctcggcgag ctttactacg
      481 ggttcgagct tggagacgtc agcgtcgagc gcacacaggc gctcaaagaa ttactcgaac
      541 tgttccagac ggaggatttt cctgccgatg ccgcgcgagc ttatggggaa atccgggctg
      601 cgctcgaacg ggccgggcgc cgaatcggcc cttatgactt gctgatcgct tcgcacgccc
      661 agcacctcgg ggccgtgctg gtgaccaaca acgagcgcga gttccggcgc gtgcccggcc
      721 tgtcggtgca gaactggctg aaagcgtgac acggtggccg ctggcgccgg ccggtgaggc
      781 gtgacgtgat acagcgcgac agggtgaagc gtgacgtggc ggggcgtgac gtggcggggc
      841 gtgacgtggc gaggcgaaat gtgagagacc gggttatgca aatagccaag cctgacccct
      901 tcgtcacccc ggtagcgata gggcggggca gaccacagat tctttcctca agtacaccgc
      961 cgacagatca agttcgttat tgggagtgac gaactcacct ggcaagacgt aatattatgg
     1021 atatgcccgc ctctactcgt gctaggaaag ccaccgaatc agccgagtcc gaggttttta
     1081 acccctccga ctggttgccc ccggagatcg ggaagatcac caatcatcag acggacctgc
     1141 ctcgcatcgc gaaggacccc aaggcaatag cagcaattga atcacggctg tcgcagattc
     1201 ctacgtcgca cgacctctat aatcgcgatt cgcgacgcat tgatttcctg acgccgaaca
     1261 gtgtccatct cgtagtcacg tccccaccgt actggacgct taaggattat cgcgagcatc
     1321 ccgatcaaat ggggtacatc gccgactacg agcggtttct tggtgaacta gatcgcgttt
     1381 ggcaggcttg ttacgacgcg ctcgtcccag gcggacggct tgtgtgtgtg gtcggggacg
     1441 tctgcctgtc ccgccgcaag aacgagggtg aacacaccgt cgtgccgctt cacgcttcga
     1501 ttcaggagca ttgccggcgg atcgggttca gcaatctcgc tcctattatc tggaacaaga
     1561 ttgcaaacgc tgtatacgag gcggagggca atggtggcgg ttttctcggg aagccctatg
     1621 agcccaacgc tgtcgtgaag aacgacatcg aattcatcct tatggagcgc aagcccgggg
     1681 gataccgcag cccaagcgtt gcaactcgcg tgctgagcgt aatacccgca gatcgtcaca
     1741 aagtctggtt tcaacagatc tggacagggg tgacaggggc ttcgactaga gatcatccag
     1801 cgcccttccc cttggagctg gctgaacggc tagtccgtat gttttccttc gtaggtgaca
     1861 ccgttttaga tccattcttg ggtacaggta caacgagcgt tgctgccgca aaatggggac
     1921 ggaatagcat tggcgtcgag gtggacccac attattttga aatggcggct tcccgacttt
     1981 ccaacggtag agagctattc tcagcaacaa cgatacggtt gatgagaggg gagaatggac
     2041 aggttcgagg agatcgttca gtcgggctta cggcaagcga ttcggcatta ctggcttacg
     2101 agaacaaagc aaatagacgg ccaacaggcc gggtcggttc gagaccaagg acgacgagga
     2161 gaagtcaccg gaggaaagca gcttgacggc tttctcacgg taattcgaga tgtccttgcc
     2221 ttaggcggaa tcaagaaacc cgaagtattt accggacaga ttgattccta tctgcccggc
     2281 tacttccgac ccacgaaaag gtgggacctt cttgcaattg cggacggtaa ccttcttgcc
     2341 tgcatcgaga tgaaatcgca ggcggggcct tcctacggaa acaatttcaa taaccgtgtg
     2401 gaagaggcca tcggaaatgc gcacgacttc tggagggcat accaggaaca tgccttcaag
     2461 acccaaacaa agccttttct tggctacatc atgttgttag agcatgacga agcctcgaca
     2521 aaaccggtgc gcgagaacga accacatttc aatgtacgac ccgaattcaa gaatgcctcg
     2581 tacgcgaggc gctacgaact cttctgtgag aaagcgtttc gcgagggtct ttacgacgcg
     2641 acggcctttc taatgtctga aaaggatggt ggattggatg gtgcatacat cgagccttca
     2701 aaggatcttt ctttcaagaa tctcgcagcc gccctgtatg gccgggctgt tgcttattct
     2761 aagcaaatag ggagaggttc ctgagtgctt tgctgacatt cgcaaagatg ttttggcggg
     2821 atcttcaatt caatgctcga agaagcaatt aattcactgc tgggcaagaa gcagacttct
     2881 acgaagcgtc ttacgcttct ggctgaccat ttgaagcaca tgcttgaggt ggcagggttg
     2941 ccaaacgttc aaggtgggac tggtggtgaa ctgcgagttc cagggctggc gcgatccaag
     3001 gattgggaca tcgcctatga attcgccggt aagttcaggc ttctgatttc acttaaatca
     3061 atgctgggaa atatttcagg ttcggtacca aacaggcttg atgacttgca aggtgaaatt
     3121 gcgaacatcc aacagttacg cccggaaatc gtgattgggt atgtggttct ttttgatgtt
     3181 gcaaaagatg cacgtcggaa agatggaacg ctatggtcgg atttctttaa atcttcgatt
     3241 caaaagatat ctattcgtcg tgcgccatta tggaaccaag gattgcttga ggcatcatgg
     3301 ttcatccggt ttaacagcaa gcttcctatg ggccgtcgcg tcgtcgcgcc cgaaaatgta
     3361 gagaaggaag ggcgttactt cattagcgct cttttgtctg agttgaagct tcgagagcca
     3421 gcgatccact tctcgaagcc aattagataa atccgatggg cttcctggat aggtagatta
     3481 ggtctcactt cttgccgaaa tatcgcgcca aaccccggcc acgacaaatg ggcaagccca
     3541 tttgtcaggg atgagggcgg aggagttagt gctggtgatg agtgatgggt gatgagtgat
     3601 gagtctcccg cgcctacttc aaaaccttgc tcgcttccgc cgcctttatc agcgcctccg
     3661 cgtccttctg cagcaggaag ccctgcgcca tcgcattcgc cgctgcgttg cgcaccgccg
     3721 ccacgtaacc cgcgtggtcg ccgtagcgct cttccagcga cgggcggggg tcgttggcgg
     3781 cttcgcgctc ggcccgggtc ttcgcgaagg ggatcatgcc gcccgcgtag ttgcagagct
     3841 tgccctggtg gaaggggcgc gcgccgccgg ccgtgatgtt ccagccaagg taggtgccga
     3901 gcggcgcgtc gagcagcacc accggcacgc cgccgacttc gttgccgtcg gcgttcacct
     3961 tcggcgccag catcttcagc gcttgccgga tcttcggcgg cgcgttggcg ggcacgcccg
     4021 acgcgtcgtt ggggtcgaac cgcgggcccc agtcgtagtc gagcagcggg ttgatgaaat
     4081 ccggttccgg cgcggtgggc cgtaagcctg gaatcgtcgg gaagcccatc gccgccttgt
     4141 ggggcgcgac gagctcgccc gcccgcatcg tcgggtagcg gctcggcggc ggcggcgtgc
     4201 cgcgcatgac ccagcttctg aaatgcacgc ggatcgcgtt cagcgtttcg gtgtgcggca
     4261 gcggattcgc gggcagcacg ccggcgcccc agttattgcc ggggcaggcg acgggcttcg
     4321 cggccttgtg ggtctcggcg aggctggtgt cgaagccgcc gcgcccgccg ccgtgcgggc
     4381 tgctcgcgat gtagtagcgg cgcacgttgt cgggcagcgc gatgtcctct ttcgggtcag
     4441 tgcccaccca ttcgggcccg agcttcagcg cccagatttc ggccgcgccg aaatgctcga
     4501 tgatcttcgg gcaagtcctc gtggccgtgc agcggtcctg gatgctgcgc gtgggcagat
     4561 tccgcgcctt gtcgggatac gggccccacc attgcgggcc ttcgctgccg gcctggtaca
     4621 gctcgagcac gccgtcgggc tgcgcccacc ggaaattgag cgcgatacgc cgcccggcga
     4681 tgatcggcca catgccgtcg tgcacctggc gcttcgcttc gtcctggttg aagcccaggt
     4741 gcagccagcc gcgcaggtag ttgcccgatt gcgagcgccc gcgcgcgatg ctgtgagtga
     4801 tgcgcccgga gatcgggttc ggcgtgccga agtcgtcctg cgcggcgtat ttaaagaacg
     4861 atcccacgtc gcgccacgcg gcgaagccga tgccgagcgg gtacgggtct ttcgcggtgt
     4921 acaccacctg gtagagcttc gccgcgtcga agccgccctt gaggcaaatt tgcaccggca
     4981 gcttcgccag cggcacgggt tcctcgaacg tgccgccgcc gcagaacttc cagtcttcgg
     5041 gcgcgatctt cctctcgccg gtgaccttgc cgccgatggt ttcgtggtcg cgcgaaacga
     5101 gcgcggccgt cgcggtgtcg aggctcgctg gcaggtacgg caccgggttg gtctgcacga
     5161 tcagcggctg cgcaccggcg ccggagcggt tcacgatgcg cgcgaacacc gggccggtga
     5221 tcggcgagcc atcggggttc ttcgcaaccg gcagctgcag ccagtggttg ccgcccgccg
     5281 ccatggccgg gcgcaccgcg gtgccgagcg cggcggccat gctggcgttg tcgccctgcc
     5341 aggcgctggc gagcccgatg tcgccgaact cgcgctcgct cgtgtcgatc agcaccgggc
     5401 ggccacggtt gggcacgtcg tgccacatga ggccgctcgc cttgccgatg tcgacgggcc
     5461 gggtgatgac aaaggtcgct acgtagcgaa ccttgccgtc cgcatccctg gcgagcgcga
     5521 tgtcctggat caggccgttc agcggatccg cggggtcgag ctcgccccag gcgcgcccgg
     5581 caatctgctc gtactggccg gcctcgccgt agctcgcgca ggcgccgccc ttgcagaagg
     5641 cgggcgacac ggtttcgtcg atgacgattt tcgtgatgcg ggcttgcgcg ggcgcggcgg
     5701 ccagcagcgc ggcggcaaag gcgcaggcaa gcgtgcgcac gggccgcatg ttcggacggc
     5761 aggacgtcat gaaactctcc ccctgaaagt gcggtgctga gctgccgcgg tgcttttttg
     5821 cggactttac cccattttga tgaagcgtga tgagtgatgg gagaaagaca aggatgaagg
     5881 cggaaggatg aaggatgacc taagaccggg tgatgggtga cgagtgacga atactaactc
     5941 ttattccacc agccccaggt tgctgcctgg ctcacatcag ggattttgtc gaccacgacg
     6001 ttgacgatgc gcccgccctg tctctcggtc ctcgtgatgt aaatcggggt gattgcctgc
     6061 cggtacgcgt cgaaccgcat tggcccgcgc ggtggcttga ttcgtggcag cgcgctcgcc
     6121 aacgcatcgc gcactcggcc gcggtcgctc acgtcgccgt tgagcgagtc gagtgcggtg
     6181 gcgatgaggg cggccgcgtc ccagccgcac tcgctgtagc ggctggggac ggcccggaag
     6241 gccgcctcgt agtcccgcac gaactgcctg ttttccgggg tatcgatggc ggcgctgtag
     6301 gccccgactg cgacgatgcc gagggcggcg tcgccgatcg tgggcaggat ggtctcgacc
     6361 gtcagcgcgg aatggcccgt gagcggcagc tgtcggccga tggcggactc ccggtactgc
     6421 ttcacaaaat tgaccgcatc ggccccggcg aaccaggcgt acgcggcgtc ggcgcgcagc
     6481 gagccgatcc gggcgagata cggcgagaaa tccggcgtgt cgaacggcgg gtagatttcg
     6541 gcgacgatct ccccgccgcc ggcgcggaag gcagccatga acgcctccac cgagtggcgc
     6601 ccgcccgcga aatcggccgc catcacgatc atcctgcggt agggggcgtg cttcatcatc
     6661 caggcgccca ttgaatagtt cgcctggtcg ctggtctcgc tcaggcggaa gatgctcggt
     6721 gatgcctgct gaggcgaggt gagcacgcgg gtgaaggccg ccggcaccag gagcggtgtg
     6781 ccctggtcgt gtatgtagtt gcggatggcg agcgccaccg cgctgctgat cggcccgatc
     6841 aggaaatcga cgcgggagtc ctcgaccagc ttcttcagcc gggcgagcgc cacggcgggc
     6901 ttggcctcgt cgtcctcctt gaagaggcgg atctcacgcc cgccggcgcg gtagccgatc
     6961 ttcccgaggt agagctcgaa ccctttggtg gcgtcggtcc cctgcgcggt gtccacgccc
     7021 gtggtcggca gcagcaggcc gatcttcacc ggagacgaat cggccatggt cgtggaaaag
     7081 taggttcaga ctcgactttc ccggaaagta tcgcgccaaa ccccggccac agccaaatgc
     7141 gcacgcgcat ttgggaaggc gaaaggcgga aggcgaaaga cgaaaggaaa gataggtcga
     7201 aggccgaacc ggcccggact gtgtcgttac ttccgccttt cgactttcgc ctttcgcctt
     7261 gccttttaac ccatcggggc ggattcgggt cggacctccg caacccattg agtcgcgtca
     7321 gtaagcgcgt cctatcgggg cgttttcagc ccctggcggg tttattggac acaaaaacgc
     7381 acgcttcaag aactcatccc gcaacggctt ggaccacacc gttgagcgat ccgctaggct
     7441 cgtaaccgtt cgagcgaacg ctcaatattc gagcgcgcct gctgctcgta acgatcgcga
     7501 aagtgtgtgc tgctgaaaat ctgcgggcgt gccaggaatt ccttcaggat ggccttgcgc
     7561 tcccgccgga acaggaaatc gggtacccag gcgtattcct cccgcacttg ccgttcgtac
     7621 tggtcgaatc gctccggtgc ggtgccgagt atcgatagat caacgtccac gagtatctcg
     7681 gcgtccgtgc cgcggggaac tgctttgtga cgggttgcga tgacaaggga gtgcacgcga
     7741 ttgcacgcct cggcgggaat cccggctgcc aaggcgctgg cgcgggccca gtcggcgctc
     7801 tttttctcgt tatcgcgccg cttcacgtcg tagatcgcgt cgtgaaacca caatgccagt
     7861 tcgatctccg ccgggtgctc ggccagcgcg cggacctcat ccagcttttc gaagcactcg
     7921 tcgagatgct gcagcgtgtg gtacttgcgg tgcggctcgc tgtaccgggc cataagctcg
     7981 ccatgcagct tcgggtcggg atttgctgca ccaagctcct tccacatttc gtgccatcgg
     8041 gcaatggtga gcatcgcgga ttttggatca gactcggcct tcctcgaagt atcgcgccaa
     8101 accccggcca cagccaaatg cgcacgcgca catttccgtg agggcgggag ggcgtgagag
     8161 cgagagaggg ataagttccg tgagggcgtc agggcggtag agcggaaggg gaaaccccct
     8221 ctaacgattt tccgatctcc cgacctcccg atctgacgat ctcccgggtt ctcgggtcga
     8281 tccacggtcc acgacccacg cagtccggac tgcgggcgat ggatgagggg gcgaaaagag
     8341 actaatcgtg cacggcctct tttgcgggac tctccgcgcg cgcgaggtgc cggcctgcga
     8401 catagccgaa cgtcaacgcc ggcccgagcg tgatgcccgg tcccgggtag ccgccgttca
     8461 tgatcgtgtt catgtcgttg ccgcaggcat agagcccggc gatcggtgcg ccggcggcgt
     8521 cgagcacctg cgcgtgcacg ttcgtgcgca ggcccgccgc ggtgccgaga tcgccggggt
     8581 acagggcgac cgcgtagaac ggggcccgct cgagcggcgc cacgcaggga ttgggcgcgt
     8641 ggtcggcgtc accgaggtat ttcccgtacg cgtcgctgcc cttgccgaac gcccggtcga
     8701 cgcccgtgcg cgcatcctcg ttgtagcgca gcacggtcgc ctccagcgcc gccgcgtcga
     8761 tgccgagcgc cccggccagc gcgcggatcg aagatccccg cgcgaggtag ccgcttgccg
     8821 tatgcgcgcc gggaaacgcc gtaaacggct tcacggcccc caggccgtac ttccacaagc
     8881 tcgcccggtc gcagatcagg aaggctggaa tcgcatgcgc gcggaacatg gcccggacga
     8941 actcgtggta ggagacggcc tcgttggtga agcgttgccc gcggcggtcc acggcgatgt
     9001 agccaggctt gccgcggtcg gttaccgaat ggggatagac cgccggcgtc ccgtccgcgc
     9061 ggtggaagcg cgaaaccggc gcccagtacg cgttgtccgt gttctcctcg accatgcggc
     9121 caccggccgc gaggccaagc cgcagcccgt cgcccgagtt cgccgggcag gccgcggacg
     9181 atggcccggt ttcggcgggt agataccggg cgcgcagccc ggcgtcgttt gaaaatccac
     9241 cggccgccag gacgaccccg cgccgcgcac gcaagcgggt gccgtccgcg agcgtgacac
     9301 cggcaacgcg cccgccctcc atcgcgagcg acgcgacctg cgtcgacgtg cgcagcttca
     9361 catcgagcgt cacgaccgac ttgagcaggc gcgccgccag cgcgttgccc agcacgagat
     9421 gcgcgccgcg gtcgaacgag aggcgatcgc gcgcatggcg cgccgcgacg cgcgcgacgc
     9481 gcagcgccga tggaagcgag cgccacgcct tgcggaaatg cggtatgtcg gcgcggtcga
     9541 ccatcatgcc gccgaacatc gtgaactcgg gtagcggcgg acgcagcagg ccgaagtggc
     9601 gtccgagctc gcgtccgtcg aacgggaccg gttcgagcac ccgcccgccg agggtcgcgc
     9661 cgggcaggtc ggggtagtaa tccggataga acgtcacggg cttcaaggcg agcgccgtgc
     9721 gcgcttcgag gtactcgatc gcctcgggcg cgtgcgtgag gaagacctca cgcagctcgc
     9781 ggttgaagtc gccgggaacc gtgtggctca gatagagccg ggcctgctcg agcgaatccg
     9841 tgagtcccgc cgcgcgcatc ttcgcgttgg cggggaccca caccatgccc cccgagacgg
     9901 aagtcgttcc gccgacacga tccgccttct cgacgagcag cgtcgcgagc ccctcggccg
     9961 ccgcgacaca cgcggcggtc atgccgcccg cgccggcgcc gatcacgatg acatcatggt
    10021 cctcggagaa attcatacct atggggaaaa ggtgtcagga acctgcaaga gaagcagggt
    10081 cagactcgac tttgctggaa agtatcgcgc caaatcccgg ccaagccaaa tgtgcgcacg
    10141 tacatttccg tgagggcggg agggcgtaag agcgggagag ggataagttc cgtgagggcg
    10201 tcagggcggt agagcggaag gggaaacccc ctctaacgat tttccgatct cccgacctcc
    10261 cgatttcacg acttcccgtt atttcgatct cccgacctcc cgatctgacg atctcccggg
    10321 ttctcaggtc gatccacggt ccacgaccca cgccgctcta agagcaggga tgagggacga
    10381 taagtttgca gttggcagtt tgcagttgcc gccaactggc ggctgacagc tgaccgcttg
    10441 cggctagcgg cagcgttccc agctcgcctc aacgtttcgt gtcctcacca tatttttcct
    10501 gtgctggcga gtacgccgtc ggcgcttccc gcgccatttt cgtgaaggcg cacagctcct
    10561 cgagccagcg cagccggtcg atctccggga ccctcaggta ggcgcggatc ttttcctcgg
    10621 tggtctcgta cgcgtagtcg atcttcggca cgctagtttc ccttgcctgt cacccgcgca
    10681 acgatcttga gcgcttcaat gtcggaggca tcctgcttgc ggccggtccc
//
LOCUS       DBNGWZ010000482        13844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3087167, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000482 DBNGWZ010000000
VERSION     DBNGWZ010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3087167"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..849)
                     /locus_tag="AC16CX_14895"
     CDS             complement(79..849)
                     /locus_tag="AC16CX_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3013830.1"
                     /translation="MQVRFDKEGAIATLTLDRPDRLNAMADPMWDALYEHLGMIAADD
                     EVRAVILIGAGRAFCSGGDVSGMAKSDIVSGRTRSKRRHRTIQALYNLEKPVIAAVRG
                     AVYGIGNALALACDLVVASETAKFSMAFKKVGMVPDGGVIFFLTRHLGIGRAKDLVYT
                     ARVVPADEALKLGLVVRVVPDEKLEAEARALAGEMAESATYALALAKKMFHSMCVPTL
                     EQLMEMETLASGIVRLTHDHKEGVDAFREKRPPKFQGR"
     gene            complement(892..2166)
                     /locus_tag="AC16CX_14900"
     CDS             complement(892..2166)
                     /locus_tag="AC16CX_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013831.1"
                     /translation="MNPAGSNPSSDAAWLYELCVARVGFAMMFTAYSGVLPLLRAEWN
                     LSAGEAGLIQSAWHLGYLVSLFVAGFLSDRFGAKRTFLAGNLFAAASAMLFALFTDGF
                     VSALLLHGLLGLCSGGSYTPGLTLISERFSPYSRGRAMGFYLAAASLGYAVALLLSGA
                     LVASIGWRGVFIATACGPFAAACLAFYTLRETPNIVHPAPAGHGAGPSLMEVLRNRPA
                     MLLIWAYTFHSWELLGMWAWLPAFLAISAAAGGDVTAQAVGLGALLSAITYISSMAGS
                     IAGGSLSDRWGRSAVILMMSCASLACSFAFGWMLAWPLWLLLIVAIIYNVTAIGDSSV
                     YSTAVTELVPPRYIGAAYSIRSVLGFGAGAVSPWTFGLVLDWGRGGPLHSESWAWGLA
                     WTSLGIGAVLGPLMTWQLRRMPEAVRMAGGKR"
     gene            complement(2330..3235)
                     /locus_tag="AC16CX_14905"
     CDS             complement(2330..3235)
                     /locus_tag="AC16CX_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3013832.1"
                     /translation="MVEGKVVVVTGAGRGIGAAIARLMGQHGAKVVVNDIGVSLSGEG
                     GDKAPADEVVIEIRRAGGEAVASFDSVAGFASAGKIVQCAVDTFGRIDCVVNNAGILR
                     DVIFHKMTEEDWDSVLNVMLKGAFNMCRHAADHFRKQESGSFVHMTSTSGLVGAMGQV
                     NYGAAKLGMVGLSKNIAIDMQRFNVRSNCIAPVAWTRMTASIPPKDPAAAKRIEQRKQ
                     VTPEYNAPLAVFLASDAAKEVTGQVFSVRKNEIFLMSQSRPLRGIHRSEGWTPETCAE
                     HMLPALKSWFFPLDKTADVFPWDSI"
     gene            complement(3373..4233)
                     /locus_tag="AC16CX_14910"
     CDS             complement(3373..4233)
                     /locus_tag="AC16CX_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3013833.1"
                     /translation="MAINYDRLISWDIPEVEQHLTRRDTILYALGVGLGADPCDASQL
                     RFVYEDNLQALPTMAIILGSPGPWHAHPELGITRSHVLHGEQGFRIVKPLPVEGNIVG
                     KTRVASVVDKGKDKGALIMTECTVRDKASGEVICTLTSTSFCRADGGFGGPDGPVRAP
                     HPIPDTPPQHVCDLPTLPQAALIYRLSGDYNPLHADPAYARKAGFEMPILHGRCTFSI
                     AGHAILKTLCGYDPARFIAMEGRFSSPVYPGETIRTEMWRGGGIVSFRSTVPARGVTV
                     LNNGRAEILT"
     gene            complement(4374..5525)
                     /locus_tag="AC16CX_14915"
     CDS             complement(4374..5525)
                     /locus_tag="AC16CX_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HIR3013834.1"
                     /translation="MSLKRASVAVVGAAESDLGQVAPNTSPVDLMAQATMRALDDCGL
                     KLPDVDGIFSAATQVRTAPMTLAEYLGIKPRYFDGTAIGGSSFMAHVAHAQAAIEHGL
                     CEVALIAYGSTQRSVSRAAASPREFNPYESPYRPFLPSSAYAMAASRHMYQYGTTREQ
                     LAEVAVAARKWALLNPKAWEKEPLTIEQVLKSRMVSYPFTVRDCCLVVDGGGAIILTP
                     AARAKSLKKKPVYVLGVGESLSHANISSMPDLTVSAAAESGPKAFGMAGVKPSDVDMV
                     SLYDAFTITPILFLEDLGFCPKGEGGRFVSNGAIAPGGRLPVNTSGGGLSYCHPGMYG
                     LLVMIEAVRQVRGECDKRQVKDCEISLAHGNGGVLSSQCTVIFGSDATL"
     gene            complement(5522..5962)
                     /locus_tag="AC16CX_14920"
     CDS             complement(5522..5962)
                     /locus_tag="AC16CX_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR3013835.1"
                     /translation="MAMDLKQQSPYGTYLEHVKRGELAYQVSVDDGKPVFYPRVVAPK
                     SGNPGLEWRVSKGLGTVYATTVVYYKGEPPLNVVLIDLDEGFRMMSRVEDIDPLEVKI
                     GMRVKVRMHPGDEKQAPYPVFTAMEGSQHHPGSSSHPSSVRRGQ"
     gene            complement(5974..6834)
                     /locus_tag="AC16CX_14925"
     CDS             complement(5974..6834)
                     /locus_tag="AC16CX_14925"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013836.1"
                     /translation="MEAFLDRPDGSKLFYRTDDYTDPWRPAETVLFVHGLAESGEAWR
                     GWVPYFARRYRVVRMDLRGFGRSTPMAADYKWSMDALLDDIEALIRRLGCDHVHLVGA
                     KSGGSMALKLAAERPTLVKTLIGVTPPLTGAADVGRWLERIETCGVLDWARHTMQGRL
                     GSVVSREEVDWWVENLQGRTPLSTLQGYLRWVPGLDIRTDVERITCPTLVITTTGSGL
                     RSVEGVKAWVARVKRARLLVLEGDAWHAAGAYPDRCAQATVEFLAEHTARQVGQRRQA
                     RRRGSRLTQP"
     gene            complement(6855..8489)
                     /locus_tag="AC16CX_14930"
     CDS             complement(6855..8489)
                     /locus_tag="AC16CX_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3013837.1"
                     /translation="MARWQKKTLAQALARSARRHPDQEALVIDGRRLTYSQLWNSVKQ
                     VAAALHALGIRRGDHVAVCTGNSLEWVQFFYGAAAIGAVTVPVNTRFKADELRYCLKQ
                     SDARLLLVADRFLKIDFIEILRDVCPAVDRRLPHRDLPLLEHVVVLGADVPKAAIRWE
                     TMLALGDKGRGLELPQVLPDDVLLIQYTSGTTSYPKGVMLSHDNMLRNAACVVERFDL
                     KAGDRYYSARVFYHVAGTTLSLLAALVAGACLLSSSHFEPGEALRLMSAEGCTHTSGN
                     DTMFVMMLNHPDLDRFPLKLRGGWASAGPEISRRAIERMGMAGLCHAYGLSETSPNVC
                     MSWHNDDLENRINGWAHLQDGVKVRIVDPETGRDLASGETGEILVRGWSVMKGYYRMP
                     EQTAKAIDRKSWLHTGDLGVMDEAGRLRFLTRIKDVFRVGGENVAPAEVEEVLHQHPA
                     IKQAQVIGVPDPRLVEVPAAYVILKDGAAASPEEIIAWGRERMANFRAPRYVRIVDSF
                     DDVGMTGSAKVQKNRLRAQALIDFGLESSARPAAAA"
     gene            8636..9136
                     /locus_tag="AC16CX_14935"
     CDS             8636..9136
                     /locus_tag="AC16CX_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR3013838.1"
                     /translation="MIKVGDKLPEATLNQLTVDYTQGCPTAPEAFKVSDLTKGRRIVI
                     FGLPGAFTRTCSGKHLPGYVELADKIKARKVDEIWCLSVNDAMVMAAWGKDQKVGDKV
                     RMMADGSAAFTRAMGLDQDLTERGFGVRSQRYSMLVDNGVVKVFNLEKPGQFEASSAE
                     KMLERL"
     gene            9166..9639
                     /locus_tag="AC16CX_14940"
     CDS             9166..9639
                     /locus_tag="AC16CX_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR3013839.1"
                     /translation="MTLTLTSTAFSHNNAIPKRYTCQGEDVSVPLAWSGVPAGTKSLV
                     LIVDDPDAPDPKAPRMTWVHWVLYNIPPSAAGLKEAVKSAELPKGTMEGFNDWKRTGY
                     GGPCPPIGRHRYFHKLYALDTVLPAMENPTKADLEQAMKGHILGKTELIGTYQKS"
     gene            complement(9636..10613)
                     /locus_tag="AC16CX_14945"
     CDS             complement(9636..10613)
                     /locus_tag="AC16CX_14945"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013840.1"
                     /translation="MKILLLPAVFLAVSISGAPTFASDSSSSFPSRPIRLIVTNAPGS
                     SVDTLSRIVAARMGDVLGQQVVVDNRPGAGSVLGMEIGKNANPDGYTMVSAPTAAVAV
                     APLVHRKVPYDTLNDFAYISLFAVTPNVLVVNPGLPVKSVRELIDYCKARPGQVNMAS
                     AGPGSQSHLTGALLTTLGNFPSLHVPYKSGGASVSAVIAGESQWTMTPAPAVWSLVKA
                     GRLRAIAHSLPRRSALLGDLPAVAETIPGYDYSGWQGLLAPKGTPKPILDKLRAALLK
                     TVSLPEVRDAIAAQATEVVTAAPEEFRKLARDELAKYGKVIKAIGLKVD"
     gene            10774..12450
                     /locus_tag="AC16CX_14950"
     CDS             10774..12450
                     /locus_tag="AC16CX_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013841.1"
                     /translation="MPKTTGPRFVAEALRAYGVTHVFFVPQMLLETLTAMEGMGIRRI
                     MTHGEKAAAYMADGYARASGRPGVCMAQHVGASNLAAGLRDAYLACSPVIAMTGGPAA
                     ARYRHAYQELEDFTQFDHTTKFNARVDHVARLPDLLRQAFREATSGAPGPVHLQIGGH
                     HAEAVMSEAELELLVEERFKQVPSFRPAADDAQVKEALGALDAAERPIIVSGGGVTWS
                     GAQQEVVALAEKLSIPVATSLNAKGAILDTHPLAVGVVGTYSRACANRAVAESDLVFY
                     IGSHTGGQVTARWQIPKPGKPVIHLDIDAREIGRNYPTRVALLGDARTVLRQMLAAAG
                     AGSGAKRGPWLARVKTLVEEWRNSVSGNVRSDAVPMRPERVCREITRVLPESAILVSD
                     TGHSGIWTGSLVEFTRPGQRLVRCAGTLGWGFPGALGVKCALPDIPVVCFAGDGGFYY
                     HLAELETAARYGINVVVVVNNNGALNQEIPHFDKAYGGDPDERGREMWGFTPVNFARV
                     AESLGCMGIRVEQPAQMAAALEKALAARRPVVIDTVTDHRAFSQKTWTGA"
     gene            12626..13549
                     /locus_tag="AC16CX_14955"
     CDS             12626..13549
                     /locus_tag="AC16CX_14955"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3013842.1"
                     /translation="MHGSLDQLKIEVDMLAPGAERIVGKNPPLDIIARDIIFGEGPVW
                     DARNKQFFFTDIIGDTIWKWKPGGKRRPVLRPSVKANGMALDLENRLLVAGWGGRTVF
                     RFEKNGSRKTLADRWEGKKLNSPNDIVVKSDGSIWFTDPPGGLLNVGMVGGDLQRYLD
                     IQGVFRIAPDGKEMTLATADFVYPNGLCFSPDEKLLYVNCSRERVIRVYDVRPGGSLG
                     QGRLFHQYVSPERGVPDGMKCDVEGNVYCTGPGGIFIHNAQGKVLARIKTPGHHPTNF
                     AWGDDDWRSLYITMIGSVVRTRLNVPGVPSR"
     gene            13561..>13844
                     /locus_tag="AC16CX_14960"
     CDS             13561..>13844
                     /locus_tag="AC16CX_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013843.1"
                     /translation="MTWKFDLVAGPYKGRTGGLAWDGAAMLFSAVMEERILRYEPGTG
                     KTGIFRKYTGRTNGIAVAADGSVFGAQEGGRRVVHFLKDGSTASMSDLLDG"
BASE COUNT         2435 a         4484 c         4555 g         2370 t
ORIGIN      
        1 gcgccgagcg cctcagcttc gttatccagg agatccacgc tggttcgggt cttcagttca
       61 aacatcaccc atcactcatc accgcccctg aaacttgggt ggccttttct ccctgaacgc
      121 gtccaccccc tccttgtggt cgtgggtgag ccgcacgatg ccggacgcga gtgtctccat
      181 ctccatcagc tgctcgaggg tcggaacgca catcgagtgg aacatctttt tggccagcgc
      241 cagcgcgtag gtcgccgact ccgccatctc gccggcaagc gcccgtgctt ccgcttcgag
      301 cttctcatcg ggtacgacgc gcaccacgag accgagcttc agcgcctcgt cggcgggcac
      361 gacgcgcgcg gtgtagacga ggtctttcgc tctcccgatg ccgagatgcc gcgtgaggaa
      421 gaagatgacg cctccgtccg gcaccatccc gaccttcttg aaggccatcg agaacttcgc
      481 ggtttccgag gcgacgacaa gatcgcaggc gagggcgagc gcgttgccga tgccatagac
      541 ggctccccgc accgccgcga tcacgggctt ctcgaggtta tatagcgcct ggatggtgcg
      601 gtggcggcgc ttcgagcgcg tgcggcccga cacgatgtcg gacttcgcca tgccggagac
      661 gtcgccgccc gagcagaagg cgcgtcccgc gccgatcagg atcacggcgc ggacctcgtc
      721 gtcggccgcg atcattccca ggtgctcgta gagcgcgtcc cacatcgggt cggccatcgc
      781 gttcaaccgg tccggccggt ccagtgtcag cgtggcgatt gcgccttctt tgtcgaatcg
      841 aacctgcata attggaaaac ggattttatc gccgcctggc gcggtgcgat gtcagcgctt
      901 gccgcccgcc atgcgcacgg cttccggcat gcgccgcaac tgccaggtca tcaggggtcc
      961 gagcacggcg ccgatgccaa gcgatgtcca ggcgagcccc cacgcccacg attcggagtg
     1021 caacggaccg ccccggcccc aatcgaggac gaggccgaac gtccacgggc tcaccgcgcc
     1081 cgcgccgaag cccagcaccg agcgaatcga gtatgcggcc ccgatgtagc gtggcggcac
     1141 cagctcggtg acggcggtcg agtacaccga cgagtcgcca atggccgtca cgttatagat
     1201 gatcgcgacg atcagcaata gccacaacgg ccacgccagc atccagccaa aggcgaacga
     1261 gcaggcaagg ctggcgcacg acatcatcag tatcacggcg gatctccccc agcggtcgga
     1321 gagcgagccg ccggcgatgc tgcccgccat gctcgagatg taggtgatgg cggacagcag
     1381 ggcgccgagc ccgaccgcct gcgccgtgac atcgccgccg gcggcggcgg agatcgccag
     1441 gaaggcgggc agccatgccc acattcccag cagctcccag ctgtggaacg tgtaggccca
     1501 gatcagcagc atcgccggac ggtttcgcag cacttccatg agcgaggggc cggcaccgtg
     1561 tcccgcggga gccggatgca cgatgtttgg cgtctcgcgc aaggtataaa aagcgaggca
     1621 ggcggcggca aacggtccgc acgcggttgc gatgaacacg ccgcgccagc cgatcgacgc
     1681 caccagcgcg ccgctcagga gcagcgccac cgcgtagccg agcgaggccg cagcgaggta
     1741 gaaacccatg gcgcggccgc ggctgtaggg gctgaagcgt tcggagatga gcgtcagccc
     1801 cggcgtataa gagccgccgg agcacagtcc cagcaggcca tgcagcagca acgccgagac
     1861 gaagccgtcg gtaaagagcg cgaacagcat cgcggatgcg gccgcgaaca ggttgccggc
     1921 gagaaaggtc cgcttggcgc cgaagcggtc ggagagaaag ccggcgacga acagcgagac
     1981 gaggtagccc agatgccagg cggactggat gagcccggct tccccggcgc tcaggttcca
     2041 ctccgccctg agcagcggca gcacccccga ataggcggtg aacatcatgg cgaaaccgac
     2101 gcgcgccacg cacaactcgt agagccacgc ggcgtcgctc gaggggttgc tgcctgcggg
     2161 attcaagcgc gatcagccac agagattaaa gagaacactg catcgtgatg cgtgatccgg
     2221 gatacgcgat tcaggatacg ggatgcgcga tgtggaattg aagccagagt tcaatcccgc
     2281 atgtcgcgtc tcgtatctcg taactcgcat ctcgtatctt gcatctcatc tagatcgagt
     2341 cccagggaaa cacgtcggcc gtcttgtcca gcgggaagaa ccagctcttc agcgccggca
     2401 gcatgtgctc ggcgcaggtc tccggcgtcc agccctccga gcggtggatg ccgcgcaggg
     2461 ggcgcgactg gctcatgaga aagatctcgt tcttgcgcac cgaaaacacc tgaccggtca
     2521 cttccttcgc cgcatcgctc gcgaggaaca ccgcaagggg cgcgttgtac tcgggcgtaa
     2581 cctgcttgcg ttgctcgatg cgcttcgcgg ccgccggatc cttgggcggg atcgaggcgg
     2641 tcatgcgcgt ccacgccacc ggcgcgatgc agttggagcg cacgttgaag cgctgcatgt
     2701 cgatcgcgat gttcttcgac agcccgacca tgccgagctt ggctgcgccg tagttgacct
     2761 ggcccattgc gccgacgagc cccgaggtcg aggtcatgtg cacgaagctg ccggactcct
     2821 gcttgcggaa gtggtccgcc gcgtggcggc acatgttgaa ggcgcctttc agcatgacgt
     2881 tcagcaccga gtcccagtcc tcctccgtca tcttgtggaa gatgacgtcg cgcaggatcc
     2941 ccgcgttgtt gaccacgcag tcgatccgtc cgaaggtgtc caccgcgcac tggacgatct
     3001 tcccggccga ggcgaatccg gcgacgctgt cgaagctggc gaccgcctcg ccgccggccc
     3061 tcctgatctc gatgaccacc tcgtccgcgg gcgccttgtc gccgccttca ccggaaagcg
     3121 acacgccgat gtcgtttacc acgaccttgg cgccgtgctg ccccatcaat ctggcgatcg
     3181 ccgcgccgat cccgcgcccc gcgccggtca ccaccacgac ttttccttca accattcgtt
     3241 tcgcattcac tgatgacccc caccaaaaat ccttgacgca aaggcgcaaa gccgcaaagg
     3301 aaagataaaa tgattttaga agtcatttta ttgtttgaat ctctttgcgc cttcgcggct
     3361 ttgcgtcgag gtttacgtca aaatctcggc acggccgttg ttcagcaccg tcacgccccg
     3421 cgcgggaacg gtggagcgga acgagacgat gccgccgccg cgccacatct cggtgcggat
     3481 cgtctcgccc ggatagaccg gggaggagaa gcgcccctcc atcgcgatga atcgcgccgg
     3541 atcgtagccg cacagcgtct tcaggatcgc gtgcccggcg atgctgaagg tgcagcgccc
     3601 gtgcaggatc ggcatctcga acccggcctt ccttgcatag gcgggatcgg cgtgcagggg
     3661 attgtagtcg ccggaaagcc ggtagatcag cgccgcctgc ggcagggtcg gcagatcaca
     3721 cacatgctgt ggcggcgtgt cgggaatcgg gtgcggcgcc ctgaccggcc cgtcgggccc
     3781 tccgaagccg ccatcggcgc ggcagaagct ggtggaagtg agcgtgcaga tgacctcgcc
     3841 gctcgccttg tcgcgcaccg tgcactcggt catgatgagc gcacccttgt ccttgccctt
     3901 gtccacgacg ctggccacgc gcgtcttgcc gacgatgttg ccttccaccg gcagcggctt
     3961 cacgatgcgg aagccttgct cgccgtgcaa gacatggctg cgcgtgatgc cgagctcggg
     4021 atgcgcgtgc cacggcccgg gagagccgag gatgatcgcc atcgtcggca gcgcctgcag
     4081 gttgtcctcg tagacgaagc gaagctggct cgcatcgcag ggatcggcgc cgagccctac
     4141 gccaagcgca tagaggatgg tgtcgcgccg ggtcagatgt tgctcgacct ccgggatgtc
     4201 ccagctgatc agcctgtcgt agttgatcgc catattatgg ggatgcgacc aaggagggcg
     4261 cgccggactc agactgccgc aggctggagc gggaaggcgc gcaagcagcc gacagcacga
     4321 aacccattcc tccctcggcg gttcggaggc tgcggccgtc gcacggtgta tagctatagg
     4381 gtcgcatcag aaccgaatat cacggtgcat tggctggaga gtaccccgcc gttaccgtgc
     4441 gcgagtgaaa tttcgcagtc cttcacctgc cgcttgtcgc attcgccgcg cacctgccgc
     4501 accgcctcga tcatcacgag caggccgtac atgccggggt ggcagtagga aaggccgccg
     4561 ccgctggtgt tgaccggcag cctgccgccc ggcgcgatcg cgccgttcga aacgaaccgg
     4621 ccgccttcgc ccttgggaca gaaacccagg tcctccagga acaggatcgg cgtgatggtg
     4681 aaggcgtcgt agagcgaaac catgtccacg tcggaaggct tgacccccgc cattccgaat
     4741 gccttgggac cggattcggc cgcggcgctg accgtgaggt cgggcatgct ggagatgttc
     4801 gcgtgcgaca gcgattcgcc cacgcccagc acgtagacgg gcttcttctt gagcgatttt
     4861 gcgcgtgccg ccggggtgag gatgatcgcg ccgccgccgt ccaccactag gcagcagtca
     4921 cgcaccgtga aggggtagct caccatccgc gacttcagga cctgctcgat ggtgagcggc
     4981 tccttctccc atgccttcgg attcagcagc gcccacttgc gcgcggcaac cgccacctcg
     5041 gcaagctgct cgcgggtggt gccgtattga tacatatggc gtgaagcggc catggcgtag
     5101 gccgaagacg gcaggaaagg acggtagggg ctttcatacg gattgaactc gcggggagag
     5161 gccgcggcgc gcgagaccga acgctgggtg ctgccgtagg cgatcagcgc gacttcgcac
     5221 agcccgtgct cgatcgccgc ctgcgcgtgc gcgacatggg ccatgaagga ggagccgccg
     5281 atggcggtgc cgtcaaagta tctgggcttg atgcccaggt actccgcgag cgtcatcggg
     5341 gcggtgcgca cctgggtcgc cgccgagaat atgccgtcga cgtcgggaag cttcagcccg
     5401 cagtcgtcga gcgcgcgcat cgtcgcctgc gccatcaggt ctaccgggct cgtgttgggg
     5461 gcgacctgcc cgagatcaga ttctgcggcg ccgacgacgg cgacgctcgc gcgcttgagg
     5521 ctcattgtcc cctccttacc gaggaggggt ggctcgaaga gccggggtgg tgctgggagc
     5581 cctccatcgc cgtgaatacg gggtagggcg cctgcttctc gtcgcccggg tgcatcctca
     5641 ccttgacccg catgccgatc ttcacctcca ggggatcgat gtcctccacc cggctcatca
     5701 tgcggaagcc ttcgtccagg tcgatcagca ccacgttgag cggcggctcg cctttgtagt
     5761 aaacgaccgt cgtggcgtac accgtgccga gacccttcga tacgcgccac tcgagaccgg
     5821 gattgccgct cttcggcgcg acgacgcgcg ggtagaacac gggcttgccg tcatccacgc
     5881 tcacctggta ggcgagctcg ccccgcttga cgtgttccag ataggtgccg tacggcgatt
     5941 gctgcttgag atccatggcc attctgtttc ctttcagggt tgcgtcagcc ggcttccgcg
     6001 gcggcgagct tgacgacgtt gtcccacttg tcgtgcggtg tgctccgcga ggaactccac
     6061 cgtggcctgc gcgcagcggt ccgggtaggc gccggcagca tgccacgcgt cgccttccaa
     6121 cacgagcagc cgggcgcgct tcacgcgcgc gacccatgcc ttgacgccct ccaccgagcg
     6181 caacccgctg ccggtggtgg tgatgacgag cgtgggacag gtgatgcgct ccacatcggt
     6241 ccggatgtcg agacccggca cccagcgcag gtaaccctgc agggtcgaca acggcgtcct
     6301 gccctgcagg ttctcgaccc accagtcgac ctcttctctg gataccacgc tgccgagccg
     6361 accctgcatg gtgtggcgcg cccagtcgag gacgccgcac gtctcgatgc gctcgagcca
     6421 ccggccaacg tcggcggccc ccgtcagcgg cggcgtcacg ccgatcagcg ttttcaccag
     6481 ggtcgggcgc tcggcggcaa gcttcaacgc catcgagccg ccgctcttcg cgcccacgag
     6541 gtgcacgtga tcgcagccga gccggcgaat gagcgcctcg atgtcgtcga gcagggcatc
     6601 catgctccac ttgtagtcgg cagccatcgg cgtcgagcgt ccgaagccgc gtaaatccat
     6661 gcgtaccacg cggtagcgcc gcgcgaagta ggggacccag ccgcgccagg cctcgccgga
     6721 ttcggccagt ccgtgaacga aaagaaccgt ctccgccggc cgccaggggt cggtgtagtc
     6781 atcggtccga taaaacagct tgctgccatc gggacggtcc agaaaagctt ccaatgctgt
     6841 ggctcctttg gtgctcatgc ggctgctgcg ggcctggcgg agctttccag cccgaaatcg
     6901 atcagcgcct gcgcgcgcag cctgttcttc tgcaccttgg cgctgccggt catgccgacg
     6961 tcgtcgaacg agtccacgat tctcacgtaa cgcggggcgc ggaagttcgc catgcgctcg
     7021 cggccccagg cgatgatctc ctcggggctc gccgcggcgc cgtccttcag gatcacgtag
     7081 gcggccggca cttcgacgag gcgcggatcg gggacgccga tcacctgcgc ctgcttgatc
     7141 gcgggatgct ggtgcagcac ttcctcgact tccgcggggg cgacattctc cccgcccacc
     7201 cggaacacgt ccttgatgcg ggtgaggaag cgcaggcgcc cggcttcgtc catcacgccc
     7261 aggtcgccgg tatgcagcca gcttttcctg tcgatggcct ttgccgtctg ctccggcatc
     7321 ctgtagtagc ctttcatcac gctccatccg cgcaccagga tctctccggt ttccccggat
     7381 gcgaggtccc tgccggtttc gggatccacg atgcgcacct tgacgccgtc ctgtaggtgc
     7441 gcccagccgt tgatccgatt ctccaggtcg tcgttgtgcc acgacatgca gacattgggc
     7501 gacgtctccg acagcccgta ggcgtggcac aacccggcca ttcccatgcg ctcgatggcc
     7561 ctgcgcgaaa tctccggccc cgccgaggcc caaccgccgc ggagcttcag cgggaaacgg
     7621 tcgagatcgg ggtgattcag catcatcaca aacatcgtgt cgttgcccga ggtgtgggtg
     7681 catccctctg cgctcatcag gcgcagcgcc tcgccgggct cgaaatggga cgaggagagc
     7741 aggcatgcgc cggccaccag cgcggcgagc agcgagagcg tcgtaccggc gacatggtag
     7801 aacacccgcg cgctgtaata gcggtcgccg gctttcaggt cgaagcgttc cacgacgcag
     7861 gcggcgttgc gcagcatgtt gtcgtggctg agcatcacgc ccttgggata ggacgtcgtg
     7921 cccgaggtgt actggatcag caaaacgtcg tccggcagga cctgcggcag ctcgaggccg
     7981 cgtcctttgt cgccgagcgc gagcatcgtc tcccaccgga tcgccgcttt cggcacgtcg
     8041 gcgccgagca cgaccacgtg ttcgagcagc ggcaggtcgc ggtgcggcaa ccggcggtcc
     8101 accgccgggc agacgtctct cagaatttcg atgaaatcga tcttgagaaa gcggtccgcg
     8161 acgagcagca gtctagcgtc ggattgcttc aggcagtagc ggagctcgtc ggccttgaag
     8221 cgggtgttga ccggcaccgt gaccgccccg atcgccgccg cgccgtagaa gaactgcacc
     8281 cattcgaggg aattgcccgt gcataccgcc acgtgatcgc cgcgccggat gccgagagcg
     8341 tgcagagcgg ccgcgacctg cttcacggag ttccacagct gcgagtaggt gagacgccgg
     8401 ccgtctatca ccagggcctc ctggtcagga tggcgcctgg cgctgcgggc gagagcctgg
     8461 gcgagagtct ttttctgcca tctggccatg atttagctgg ataccgccta tcgatcgggc
     8521 atgtcaactc tcatcgccgg gtctgttcca cattgcgtaa ccggcgacgg ggctgcgcag
     8581 gcgctgggct atactaatag ccccttaatc gcaaaaaatc agccaggagg gcacgatgat
     8641 caaggtggga gacaagctgc ccgaagccac gctgaaccag ttgaccgtcg attacaccca
     8701 gggatgcccc accgcgcccg aggcgttcaa ggtgtccgac ctgacgaagg gcaggcgcat
     8761 cgttatcttc ggcctgccgg gcgcgttcac gcgcacctgc tcgggcaagc accttcccgg
     8821 ctacgtcgag ctcgccgaca agatcaaggc caggaaggtg gacgagatct ggtgcctgtc
     8881 ggtgaacgac gcgatggtga tggcggcgtg gggcaaggac cagaaggtcg gcgacaaggt
     8941 gcgcatgatg gcggacggca gcgcggcctt caccagggcg atgggcctgg accaggacct
     9001 gaccgagcgt ggcttcggcg tgcgctcgca gcgctattcg atgctggtgg ataacggcgt
     9061 ggtcaaggtt ttcaacctcg aaaagcccgg ccagttcgaa gcgagttccg ccgagaagat
     9121 gctcgagcgg ctgtgatcac ctttttgatg gcaggagcag cggccatgac gcttacgctg
     9181 acttcaaccg cgttctccca caacaacgcg atacccaagc gctacacctg ccagggcgag
     9241 gacgtttcgg tcccgctcgc ctggagcggg gtgcctgctg gaacgaagag cctcgtcctg
     9301 atcgtggacg acccggatgc gcctgacccc aaggcgccgc gcatgacctg ggtgcactgg
     9361 gtgctttaca acattccccc gtcggcggcc ggattgaagg aagcggtcaa gtccgccgag
     9421 ctgccgaaag gaacgatgga agggttcaac gactggaagc gcaccggcta cggcggaccg
     9481 tgcccgccga tcggacgcca tcgctatttc cacaaactct acgcgctcga caccgtgctg
     9541 cccgccatgg agaaccccac caaggccgac ctcgagcagg cgatgaaagg gcacatcctc
     9601 gggaaaaccg agctgatcgg cacttaccag aagtcctagt caaccttaag gccgattgcc
     9661 tttatcacct tgccgtattt cgcgagctcg tcgcgtgcga gcttgcggaa ttcctccggc
     9721 gcagccgtga cgacttcggt tgcctgggcc gcgatggcgt cccggacctc gggcaggctc
     9781 acggtcttca ggagcgcagc gcgcagcttg tcgaggatcg gcttcggcgt gcctttgggc
     9841 gcgagcaatc cctgccagcc gctgtagtca tagcccggga tcgtttccgc gaccgcgggc
     9901 aggtctccga gcaatgcgct gcgcctaggc aggctatggg cgattgcacg caggcggccc
     9961 gccttcacca gtgaccagac cgccggtgcg ggggtcatcg tccactgtga ttcgccggcg
    10021 atcacggctg aaacggatgc cccgccactc ttgtacggga cgtgcagcga cggaaagttg
    10081 ccgagcgtgg tgagcagcgc gccggtgaga tggctctgcg atccggggcc ggcggaagcc
    10141 atgttgacct gtcccggtct cgccttgcag taatcaatca actcccgtac cgatttgacc
    10201 ggaagcccgg ggttcaccac cagcacgttg ggcgtcaccg cgaacagcga gatgtaggca
    10261 aagtcgttga gggtgtcgta cgggactttc ctgtgaacca gcggcgcgac cgccacggcg
    10321 gcggtgggcg cgctcaccat ggtgtagccg tcggggttcg cgttcttgcc gatctccatg
    10381 ccgagcacgc tgccggcgcc gggacggttg tcaaccacca cctgctggcc tagaacgtcg
    10441 cccatcctgg cggcgacgat gcggctcaag gtatccaccg agctgcccgg cgcgttggtc
    10501 acgatcaacc ggatcgggcg gctgggaaag gacgaggagg agtccgacgc gaaggtcggc
    10561 gcgcctgaga tggaaaccgc caggaatact gcgggcagta acaggatctt catgaaccgt
    10621 tccattctgg ccgcttctcc cggctcccgc aacgcccggc gtgtcactat gtggtctgcg
    10681 agggcggatg gtttgtattc gcgaaggccg tttgatagca tcaaatcgct ctgccggccg
    10741 cgcgttgccg caacgcgcac agtgagaaat ccaatgccca agacaaccgg cccccgtttt
    10801 gtcgccgagg cgctgcgggc gtacggggtc acgcacgttt tcttcgtgcc gcagatgctg
    10861 ctcgagacgc tcaccgccat ggagggcatg ggcatacggc gcatcatgac gcacggagaa
    10921 aaggccgcgg cctacatggc ggatggctat gcgcgcgcat ccggtcggcc gggcgtttgc
    10981 atggcgcagc acgtcggcgc gtccaacctg gcggcggggc tgcgggacgc ctacctcgca
    11041 tgctctcccg tgatcgcgat gaccggcggg ccggcggcgg cgcgctaccg gcacgcttac
    11101 caggagctcg aggatttcac ccagttcgat cacacgacca agttcaacgc ccgcgtggac
    11161 catgtcgcgc ggctgcccga cctgctgcgc caggcctttc gtgaggcgac gtccggcgcg
    11221 cccggtcccg tgcacttgca gatcggcggc catcacgcgg aggcggtgat gtccgaagcc
    11281 gagcttgagc tgctggtcga ggagcggttc aagcaggtgc cgtcgttccg tcccgcggcc
    11341 gacgacgcgc aggtgaagga agcgctcgga gcgcttgatg cggccgagcg ccccattatc
    11401 gtctccggtg gcggagtaac gtggtcgggc gcgcagcagg aagtcgttgc gctcgcggag
    11461 aaactgtcca ttcccgtagc gacctcgctc aacgccaagg gcgccatcct cgacacgcac
    11521 ccgctcgcgg tcggagtggt gggcacctat tcgcgggcgt gcgcgaaccg cgcagtggcc
    11581 gaatccgatc tggtgttcta catcggcagc cataccggcg gccaggtgac cgcgcgctgg
    11641 cagataccca agccggggaa gccggtcatc catctcgata tcgacgcgcg ggagattgga
    11701 cggaattatc cgacgcgggt ggcgctgctg ggcgacgccc gcaccgtgct gcgccagatg
    11761 ctcgctgccg cgggtgcggg gagcggcgcc aagcgcgggc cgtggctagc ccgggtgaaa
    11821 acgctggtgg aggaatggcg caattccgtc tccggcaacg tccgttccga tgccgtgccc
    11881 atgcggcccg agcgcgtctg tcgggaaatc acccgcgttc tgcccgaaag cgcgatactg
    11941 gtgtccgata ccgggcattc cggaatctgg accgggagcc tcgtggagtt cacccggccg
    12001 gggcagcggc tggtacgctg cgcgggcacc ctcgggtggg gattcccggg cgcgctcggc
    12061 gtgaaatgcg cactccccga tatcccggta gtgtgttttg ccggcgacgg cggcttctat
    12121 taccacctcg ccgaactgga gaccgccgca cgctacggca tcaacgtggt cgtggtggtg
    12181 aacaacaacg gcgcgctgaa ccaggagatt ccccatttcg acaaggcgta tggcggcgat
    12241 ccggacgagc gcgggcggga gatgtggggc ttcacgccgg tgaatttcgc gagggtcgcc
    12301 gagtcgctgg gctgcatggg catccgcgtc gagcagcctg cgcagatggc agccgcgctc
    12361 gagaaggcgc tcgcggcgcg ccggccggtc gtgatagata ccgtgaccga tcaccgggcg
    12421 ttctcgcaaa agacttggac cggcgcgtga cgcgccacgg aaggatagcg acagcaatcc
    12481 cgagggacct cgacgcaaag gcgcaaagcc gcaaaggaag cacgacatcg aatagttatc
    12541 gaatgtattt ttgttcttgc atttctttgc gccttcgcgg ctttgcgtca agggtttatc
    12601 gttccagttg tgaagggaaa cacaaatgca cggatcgctg gatcaactga agatcgaagt
    12661 ggacatgctg gccccgggcg ccgagcgcat tgtcggcaag aatccgccgc tcgacatcat
    12721 cgcccgcgac atcatcttcg gcgaaggtcc ggtgtgggac gcgcgcaaca agcagttctt
    12781 cttcaccgac atcatcggcg acacgatctg gaagtggaag ccgggcggga agaggcggcc
    12841 ggtgctgaga ccgtcggtca aggcgaacgg catggcgctt gatctcgaaa accggctgct
    12901 cgtcgccggc tggggcgggc gcacggtgtt ccgcttcgag aagaacggat cgcgcaagac
    12961 gcttgccgac cgctgggagg gcaagaagct caacagtccc aacgatatcg tcgtcaagtc
    13021 ggacggctcg atctggttca ccgatccgcc gggcgggttg ctcaacgtcg gcatggtcgg
    13081 cggcgacctg cagaggtatc tggacatcca gggcgtcttc cggatcgctc ccgatggcaa
    13141 ggagatgacg ctcgctaccg ccgatttcgt ctatcccaac gggttgtgct tttcgccgga
    13201 cgagaagctg ctctacgtga actgcagccg cgagcgggtc atccgggtgt acgacgtgag
    13261 gcccggtggc agcctgggtc aaggccggct cttccatcag tacgtcagtc ccgagcgcgg
    13321 cgttcccgac gggatgaagt gcgacgtcga gggcaatgtt tattgcactg gcccgggcgg
    13381 catattcatt cacaatgcgc aaggcaaggt gctcgcccga atcaagacgc cgggacatca
    13441 tcccaccaac ttcgcatggg gtgacgacga ctggcgctcg ctttacatca ccatgatcgg
    13501 ttcggtggtg cgcacgcggc tgaacgtgcc cggcgtgccc tcgcggtaac ggagcggaca
    13561 atgacctgga aattcgatct cgtggccggc ccgtacaagg ggcggacggg tgggctggcg
    13621 tgggatggcg ccgcgatgct gttcagcgcg gtgatggagg agcgtatcct gcgttacgag
    13681 cccggcaccg ggaagaccgg catcttccgc aagtataccg ggcgcaccaa cggcatcgcg
    13741 gtcgccgcgg acggctcggt gttcggcgcg caggaaggcg ggaggcgcgt cgtccatttt
    13801 ctgaaagacg gctcgacggc atcgatgagc gacctcctcg acgg
//
LOCUS       DBNGWZ010000483         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_818158, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000483 DBNGWZ010000000
VERSION     DBNGWZ010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_818158"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          490 a          951 c          924 g          608 t
ORIGIN      
        1 gcggcgggcg ccagagcctg gccggattgc gtgaactggc ccaggcgctc ctcgaccacg
       61 gtctggcggc gcccgagcag gaagtaggcg gcgatgcctc ccccccacaa cagcaccagc
      121 ccgccgatga cggcgatgat gatgaccatg ttgccttgca tacgcgcctc ccgcgttacc
      181 ggataacgct caccacgctt agtaacgggt ccgttcgccg atgccgaaga ccgacggcgg
      241 caggtggatg ccggcgtctt caatgcggtc catcacccgc ggccgcagcc ccgtgggccg
      301 cagccggccc acaattctgt tcttctcaaa gccggtctgc tcaaagacgt acaggtcggt
      361 catggtgatg acgtcgcctt ccatgccctg cacttccgca atctggacga ccttgcgcga
      421 gccgtcctgc aggcgcgcca gatggacgat gaggtgaatg gccgacgaga tctgttcgcg
      481 cagggcgcgc accggcaagt ccatcccggc catcaggcac atcgtttcca gacgggacag
      541 ggtatcgcgc gcgctgttgg agtggcaggt cgtcatcgaa ccgtcgtggc cggtgttcat
      601 cgcctgcagc atgtccagcg ccgcgccgtc gcgaatctcc ccgacgacaa tccggtcggg
      661 gcgcatccgg agggcattca ccaccaggtt ctggatggtg acttcgccgc ggccctcgat
      721 gttcggaggg cgggattcaa gcgtgacgac gtgctcctgg cgcaactgca attccgccgc
      781 gttttcgatg gtgaggatgc ggtcatccgg ggggataaag ctggacatga cgttgagaaa
      841 ggtggtcttg cccgacccgg taccgccaga gacgacgatg ttgcaagccg agagcaccgc
      901 ggctttaatg aactccatcg cctccggggt tattgcgcca aattcaatca gcttctcgac
      961 cgtgagcggg gtccggaaga atttccggat cgtcaagcac gggccgacca gcgaaatcgg
     1021 cggcacaatg gcgttcacgc gcgacccgtc ctgcaggcgc gcgtcttcgt aggggtgcga
     1081 ctcgtcaatg cgccgcccca gcggggccat gatgcggtca ataatgcgca tgacgtgctc
     1141 gttgttgtca aaactggtcg gcacgcgctc gagcttgcct ttccgctcga tgtagatgtt
     1201 cttggggccg ttcaccatga cctcggtgat cgtctcgtcc tgcagcagcg cctccagcgg
     1261 ccccagcccc aggatttccg aaacgatctg gtcgaacagg cgcttgcgct cggcccgcga
     1321 gaggacgatg ccttcttcgg ccaggatgtt ttcgtagagg tcttcaatcg tcttgcgcac
     1381 ctcggcggta cgtgaaatat ctatggaggg atcgagctcg gagagcagct tgttctggat
     1441 gcgcgtcttg aggtccacga tttgcgaccc ggctgccccg ctttgggccg tgccgggcgg
     1501 cggagccgag cgcttcatgc gcagttccgc cagtttggag gcctgatctt cacccccact
     1561 cgggggagcg ccgccgccgg gttgcggggc gccggactgg cccgtcgcgc cctgccgctg
     1621 ttcgattcgc tttaagagtg acatggcaat cgctcgcttc tagacaccta ggaacttcaa
     1681 ctcacttgcg ctgaaacagg cccatcttcg tgggcggctt cttgctcttg tcctcggccg
     1741 ccggggccgg ttccttcggc gcgaattcct ccttgagtct ctgggccagg tccagcaggc
     1801 cctgcactgg cgggagattc ttcgggccgg tgatgagcgg cacgccggag ttgatcgccg
     1861 tcagcaccgc cttctcgtca atcagaatct gccccttgac cgggtgcttc agggtttcct
     1921 ccacgctggc gggcgagatg cccccgcgct tgtccacctt gttcatgatc aggatcgttt
     1981 tgtccggcgg atactccagt ttgacggcca gatcgaagaa tttcttcacg ttcgccagcg
     2041 acgggatatc gggtgaagcc accaggataa tccggtcgga gatgtcgaac agggcaattt
     2101 ccacttcccg caagaccgac gaggtatcca cgatcacgta ggcgaagctt tcgcgcaggt
     2161 tctctagaac cttcttgagc gccgagggcg tgaccagttc ggcatcttcc ggcgtcggcg
     2221 gccccaggat caccttgacc ccggagctgt gcgcgatcag cacgctctgg gtgaattcgt
     2281 catccatctc ctcggcgcgt gcgatcaggc tcaccaggct gtgcttgccc ttcagattga
     2341 aaaacactcc gatgtcgccg aattgcaggt ttgcgtccac caccaggacc ttgctgtcgt
     2401 cgctgtgcag cgcgatggcg agattcgtcg cgaccgtcgt gcacccgacc ccgcccttgg
     2461 ggctgtacac ggtgacgatc ttgccttcct ggccagggcg ccggccgccg gcgccggccg
     2521 ccgacggcgg cgcggccgcg atcatctgct cgcgctggcg gctcaactcc ccgaggcggc
     2581 ggatggtgtt gatcaactcg tcggcggagg ggggcttgga gagaaagtca cgcgcgcccg
     2641 cgagcatcgc ccggcgcagg taatccgtgt cgctgttcac cgagacgatg atgatttgcg
     2701 cgatgggcac ttctttgacg atgacctcgg tggcctggat gccgtccatg tccggcatgt
     2761 tgatgtccat caagacgacg ttcggcttgt attctttgga caactgaatg ccttcgcgcc
     2821 cggtgcgcgc catcgccacc acgtcgatgt ccggctcaaa ctgcagcagc ttgcggatgt
     2881 tctcgcgcgt ttcggcgatg tcatccacaa tcagcacacg gatctttgag tcgtctttag
     2941 ccatgcgtct acctccgcct cgctctcacg tcg
//
LOCUS       DBNGWZ010000484         6401 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2834941, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000484 DBNGWZ010000000
VERSION     DBNGWZ010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2834941"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16CX_14975"
     CDS             complement(<1..644)
                     /locus_tag="AC16CX_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013844.1"
                     /translation="MAMELTGAEIVVRCLQEEGVEYVFGYPGGAVLFIYDELFKQNRV
                     KHILVRHEQGAVHAADGYARSTDKVGVALVTSGPGVTNAVTGIATAYMDSIPMVVVTG
                     QVPTHAIGLDAFQECDTVGITRPCVKHNFLVKDAKDISITMKKAFYIAATGRPGPVVV
                     DIPKDVTTAKAEFHYPESITMRSYNPVVRGHAGQIKKAIQALMKAKRPMIYTGGG"
     gene            876..1442
                     /locus_tag="AC16CX_14980"
     CDS             876..1442
                     /locus_tag="AC16CX_14980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3013845.1"
                     /translation="MATQKELSDFFAEVERRAYKQALFAVRDDHLALDIVQDAMLKLT
                     ERYTARPLAELPLLFQRILQNTIRDFYRRQKVRALWTIPVSSLFGAEAEGDADPLETL
                     EVEDDSKFSEPPAEQLERSQVLALIEKALGRLPARQRQAFLLRYWEELDVAAAAKIMG
                     CSQGSVKTHCSRATHALAAMLKKQGIKL"
     gene            1439..1828
                     /locus_tag="AC16CX_14985"
     CDS             1439..1828
                     /locus_tag="AC16CX_14985"
                     /inference="COORDINATES: protein motif:HMM:NF023699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HIR3013846.1"
                     /translation="MSLPEHELARKIVQYLNYGVDHLESGTRARLLAARKAALSHYRE
                     KPETVLGLAWAGQAAARVTEYRFYSVRNLLAISALVLALVGVAYWQSTAPVNDLADID
                     MGLLTDELPINAYLDKGFDSWLKRSSR"
     gene            1804..2319
                     /locus_tag="AC16CX_14990"
     CDS             1804..2319
                     /locus_tag="AC16CX_14990"
                     /inference="COORDINATES: protein motif:HMM:NF022748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HIR3013847.1"
                     /translation="MAKTFVTLIVWLCFVFPAVAAQSKADTRKQVRPAWSELTPAQQK
                     ILAPLAPEWDKMDATRRKKWVAVAERFPKMKPQEQQRLQKNMADWAKLSPEQRKAARQ
                     KYQTLQKLPPDKRREVAAQWKRYQQSLAAQPELSPSDPPAPPEPSPAEAPTASPPAET
                     ATAGTPAPAAQ"
     gene            2341..2802
                     /locus_tag="AC16CX_14995"
     CDS             2341..2802
                     /locus_tag="AC16CX_14995"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR3013848.1"
                     /translation="MRPADNDGVLSASLARRGLALVYEALLLAAVLLAGSLPFVVLTH
                     GTDRLLARPLFQLYLLALAGAYFVWQWRRGGQTLAMKTWRVRLVTRAGDSPTWGHALK
                     RFLFALAGTLLLGAGFLWALVDREGLFLHDRLAGTRIIKEEVERRKSKENP"
     gene            complement(2839..3912)
                     /gene="lptG"
                     /locus_tag="AC16CX_15000"
     CDS             complement(2839..3912)
                     /gene="lptG"
                     /locus_tag="AC16CX_15000"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR3013849.1"
                     /translation="MRTLRRYLATEIVAATALVFAALVMLFAFFDLVEQIKDLGRGAY
                     TLRRILVYVLLSVPDHVYALFPVAALIGTLFALAQLVAGSEYTVMRTSGVSTARFAAT
                     LAVIGFLFATLTFVIGEFVAPPAEQLAQRVRSQAITGVVAQEFRSGLWIKDDKSFINV
                     VEVLPDSTLQGVRIYEFDGEYRLRSISFAQRGTYQSDRRWLLHDITQTRFEDMGTSVH
                     EIASARWQSVLEPDLINVLLVKPEQMSAWNLYSYAQHLKENRQKALRYEIALWTKLTY
                     PLAVLVMMVLALPFAHFQGRQTGVGAKIFAGIMLGVAFHFLNRLSSHLGLLNDWPPLA
                     SALTPTVIFLSIAVGMMWWQERR"
     gene            complement(3909..4991)
                     /gene="lptF"
                     /locus_tag="AC16CX_15005"
     CDS             complement(3909..4991)
                     /gene="lptF"
                     /locus_tag="AC16CX_15005"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR3013850.1"
                     /translation="MIFRNALLRELAVAAVATFFVLLGIVITMLFVRLLGQAAIGAIT
                     TTGVVALMGFAIINYLPVLFSLTLFISVLMTLTRSYRDSEMVVWFSCGMSLTEWIRPV
                     LMFAAPVVLVIALLSLVLSPWAVTNSEELRRRMDSRDDVSAISPGVFRESRQAERVYF
                     VEEVSGAENLVANVFVSSMQHGKQGVMVAARGFQETAENGDRFLVLLNGRRYEGEPGS
                     ADYKVYEFQRYAMRVEALESQAPRAPTTKTLSTFELLRNLTPPNLAELSWRIGLPASA
                     LVLALLAIPLSFVNPRAGRSMNLVLALLIYTAYSNLLSITQASIAQSRISLAAGLFGV
                     HAVMLVILLALFYRRLMVFSLFRIVR"
     gene            5068..>6401
                     /locus_tag="AC16CX_15010"
     CDS             5068..>6401
                     /locus_tag="AC16CX_15010"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR3013851.1"
                     /translation="MEFSIKSGTPQTGRNGCLVVGIFEPRKLSATAAALDRAAKGHLT
                     RVVRRGDMQGKAGTTLLLHSVPGIAADRVLLVGLGRETDFREKQYREAVAAAVRTLNT
                     TGAEEASLHLTELAVDRRDAAWKVAHAVTVARECAYRFTRMKSKDENAQIALRRLLLS
                     VDRAAVKRATAGLEQGLAVAHGVGLAKDLGNLPPNVCTPSYLADQARELAKRYRMKVT
                     VLEREDMERLGMGTLLSVARGSAQPPKLITLEHRGGGNRMKPVVLVGKGITFDTGGIS
                     LKPAAEMDEMKFDMCGAAAVLGTMKAAGEMRLPVNVVGVIPATENMPGGRASRPGDIV
                     KSLSGQTVEILNTDAEGRLILCDALTYAERFKPAAVIDIATLTGACVIALGHVASGLF
                     ANDDALAREAAAAGDTAYDRVWRLPLWDDYQEQLKSNFADFANIGGRPAGVVT"
BASE COUNT         1217 a         2082 c         2053 g         1049 t
ORIGIN      
        1 ccgccgccgg tgtagatcat cgggcgcttg gccttcatca gcgcctggat cgccttcttg
       61 atctggccgg cgtgtcccct caccaccgga ttgtaggagc gcatggtgat cgattcgggg
      121 tagtggaact ccgccttggc cgtggtcacg tccttgggga tgtccaccac caccggcccg
      181 ggacgcccgg tcgcagcgat gtaaaacgcc ttcttcatgg tgatcgagat gtccttcgcg
      241 tccttcacca ggaagttgtg cttcacgcac gggcgcgtga tgccgaccgt gtcgcattcc
      301 tggaacgcgt cgagaccgat ggcgtgcgtt ggcacctggc ccgtgacgac gaccatcggg
      361 attgaatcca tgtacgcggt ggcgatgccg gtcaccgcat tggtgacgcc gggcccggaa
      421 gtgacgagag cgacgcccac cttgtcggtg gagcgcgcgt agccgtcggc cgcgtgcacc
      481 gcgccctgct cgtgccgcac caggatgtgc ttgacccggt tctgcttgaa cagctcgtcg
      541 tagatgaaca acacggcgcc gccgggatag ccgaacacgt attcgacccc ctcctcctgc
      601 agacagcgca ccacgatctc ggcgccggtc aattccatgg ccatcgctcg aaaagcccga
      661 tccggaaaca tgtaacgtta ccggttgccg ccgttttggt caagcaaaat atccctttaa
      721 acagcaggat agcgcttaag tttgcgatcg cttttaggtt tgcttgcgcg ggtcgattgt
      781 tttgttaaga tgccccggac cccggggggc gggcgtggag gggcgcaccg cgggctgcga
      841 taccgcagcc actcgttcca actaaggcaa gtccactggc cactcaaaaa gaactgtcgg
      901 atttcttcgc ggaagtggag cgccgtgctt acaaacaggc gctgttcgcg gtgcgcgatg
      961 accatcttgc cctcgacatc gtgcaggacg ccatgctcaa gctcacggaa agatacactg
     1021 cgcgccctct cgccgagctg cctctgctgt ttcagcgcat cctgcaaaac accatacggg
     1081 atttctaccg gcggcagaaa gtgcgtgcgc tgtggacgat acccgtctcc agcctgttcg
     1141 gagccgaggc cgagggcgat gccgatccgc tggaaacttt ggaagtggag gacgactcca
     1201 aattctcgga gccgccggcg gaacaactgg agcgctcgca agtacttgct ttgattgaaa
     1261 aagcgctcgg gagactgccg gcccgtcaac gccaggcctt tctcttgcgt tactgggagg
     1321 aattggacgt cgcggcggcg gcgaaaatca tgggttgctc gcaggggagt gtgaaaacgc
     1381 actgctcgcg ggcgacccac gcccttgcag cgatgctgaa aaaacagggg atcaagctat
     1441 gagcctgcct gaacacgaac tggcgaggaa gatcgttcag tacctcaact acggcgtgga
     1501 tcatctcgag tccgggacgc gcgcgcggct gctcgccgcg cgcaaggccg cgctctcgca
     1561 ttaccgcgaa aagcccgaaa cggtgctggg cctggcgtgg gccggccagg cagcggcgcg
     1621 ggtcacggaa taccgtttct acagcgtccg taacctactc gcgatttctg cgctcgtgct
     1681 tgcgctggtg ggcgtcgcct actggcagag cacggccccg gtcaacgatc ttgccgatat
     1741 cgacatgggc ctgctcaccg acgaactgcc gatcaatgcc tatctcgaca agggcttcga
     1801 ttcatggcta aaacgttcgt cacgctgatc gtctggctat gtttcgtatt cccggcagtt
     1861 gcggcacaaa gcaaggcgga cactagaaaa caggtccggc ccgcctggtc ggagctgaca
     1921 cctgcgcagc aaaagatcct cgcgcctctt gcgcccgaat gggacaagat ggatgcgacg
     1981 cggcgcaaga agtgggtcgc ggtcgccgag cgtttcccca agatgaagcc gcaggagcag
     2041 cagcggctgc aaaagaacat ggccgactgg gccaagctct cgccggaaca gcgcaaggcg
     2101 gcgcgccaga agtatcagac cctgcagaag cttccgcccg acaaacgccg cgaggttgcg
     2161 gcccaatgga agcggtatca gcaatcactc gccgcccagc ctgagctgtc cccgtcggac
     2221 cccccggcgc cgccggagcc gtcgcccgcc gaagcgccga cagcttcccc tcctgcggaa
     2281 acggcaactg ccggaactcc ggcgccggcc gcgcagtaac tccacgggct tcccgcaacg
     2341 atgcgccctg ccgataacga cggggtgttg tccgcgagcc tagcgcggcg tgggctggcc
     2401 ctggtctatg aagcgctgct gcttgccgcg gtactgctcg ccggctcgct gcctttcgtc
     2461 gtgctcacgc acggcacgga ccggcttctt gcccgtccgc tgttccagct ctatctcctc
     2521 gccctggccg gcgcctattt cgtctggcag tggcggcgcg gcggccagac gctcgcgatg
     2581 aagacctggc gcgtgcggct cgtcacgcgc gcgggcgatt cgccgacctg gggacacgcc
     2641 ttgaaacgtt tcctcttcgc gctcgctgga accctgttgc tgggcgcggg gtttctctgg
     2701 gcactggtcg atcgcgaggg cctgttcctg cacgaccggc tggcgggaac aagaataatc
     2761 aaggaggagg tcgaacgtcg aaagtcgaaa gaaaacccgt aacccgctgt ctttgccttt
     2821 cgcctttcgc ctttcgcctt atctccgctc ctgccaccac atcatcccca ctgcgatgga
     2881 gaggaagatc acggtgggcg tgagcgcgct cgcaaggggc ggccagtcgt tgagcaaccc
     2941 caggtgcgag ctcaggcggt tgaggaagtg gaatgcgacc cccagcatga tgccggcgaa
     3001 aatcttggcg ccgacgcctg tctgccggcc ctggaagtgg gcgaacggca gcgccagcac
     3061 catcatgacc agcaccgcca ggggataggt gagcttggtc cacagcgcga tctcgtagcg
     3121 caacgccttc tggcggtttt ccttgagatg ctgggcgtag gaatacaagt tccacgccga
     3181 catctgctcg ggtttgacca gcagcacgtt gatgaggtcc ggctcgagca ctgattgcca
     3241 tctggcgctc gcaatctcgt gcacggaggt ccccatgtcc tcgaaacggg tctgcgtgat
     3301 gtcgtgaagc agccagcggc ggtcgctctg gtaggtgccg cgctgggcga agctgatcga
     3361 gcgcaggcgg tattcgccgt cgaattcgta gatcctcacc ccctgcagcg tgctgtcggg
     3421 cagcacctcg accacgttga tgaagctctt gtcgtccttg atccacaggc cggagcggaa
     3481 ttcctgcgcg accacgccgg taatcgcctg ggagcgcacg cgctgcgcga gctgttccgc
     3541 cggcggcgcg acgaactcgc cgatcacgaa agtaagcgtc gcaaacagga aaccgatcac
     3601 ggcgagcgtg gcggcgaagc gcgcggtgga cacgcccgag gtcctcatca cggtgtactc
     3661 cgatcccgcc accaactgcg cgagcgcgaa cagggtcccg atcagtgcgg cgacggggaa
     3721 cagcgcgtag acgtggtccg gcacggacag cagcacgtag accaggatgc ggcgcagagt
     3781 gtaggcgccg cgcccgaggt ccttgatctg ctcgaccagg tcgaagaacg cgaacagcat
     3841 caccagcgcg gcgaacacca gggcggtcgc cgccacgatc tccgtcgcga ggtaacgccg
     3901 cagcgtcctc atcgcacgat ccggaacagc gagaacacca tgagtcgccg gtagaagagg
     3961 gcgagcagga tcaccagcat cacggcgtgc acaccgaaaa gccccgcggc gagcgatatc
     4021 cgcgattggg cgatggaggc ctgcgtgatc gagagcaggt tgctgtaggc cgtatagatg
     4081 agcagcgcca gcaccaggtt catcgagcgc cccgcccggg ggttgacgaa cgaaagcggg
     4141 atcgccagca gcgccaggac cagcgcgctg gccggcaggc cgatgcgcca cgacaactcg
     4201 gccagattgg gcggcgtcag gttgcgcagc agctcgaacg tggacagcgt tttcgtggtg
     4261 ggcgcgcgcg gcgcctgcga ctcaagcgct tcgacgcgca tcgcgtaacg ctggaattcg
     4321 taaaccttgt aatccgccga gcccggctcg ccctcataac gccggccgtt gagcagcacg
     4381 agaaagcggt cgccattctc cgctgtctcc tgaaagccac gcgccgccac catgacgccc
     4441 tgcttcccgt gttgcatcga actcacgaac acgttggcga ccaggttctc ggcgccggag
     4501 acttcctcca cgaaatagac gcgctcggcc tggcgtgact cgcggaacac gcccggcgag
     4561 atcgccgaga cgtcatcgcg gctgtccatt cgccggcgca actcctcgct gttggtcacc
     4621 gcccacggcg acagcaccag cgaaagcagc gcgatgacga gcacgaccgg cgcggcgaac
     4681 atcagcacgg gccggatcca ttcagtgagg ctcatgccgc acgagaacca caccaccatc
     4741 tcggagtcgc ggtagctgcg cgtgagcgtc atcagcaccg agatgaacag cgtcagcgag
     4801 aacagcaccg gcaggtagtt gatgatcgcg aagcccatca acgccacgac gccggtggtc
     4861 gtgatggcgc cgatcgccgc ctggccgagc aaccgcacga acagcatggt gatgacgata
     4921 ccgagcagca cgaaaaacgt cgccaccgcc gcgaccgcga gttcgcgcaa tagcgcgttg
     4981 cggaagatca tctggaaacc ggatccactt tgactttttg ccaagaatgc gcaaataatc
     5041 agcaggctaa cccaattgga gcgcttggtg gaatttagca taaaaagcgg cacaccgcag
     5101 acgggccgca acggttgtct ggtcgtgggc atattcgagc cgcgcaagct ctccgccacc
     5161 gcggcggccc tcgaccgcgc cgcgaaaggc cacctcaccc gtgtcgtacg ccgtggcgac
     5221 atgcagggca aggccggcac gactttgctt ctgcacagcg tgcccggcat cgccgccgac
     5281 cgcgtgctgc tggtggggct cgggcgcgaa accgatttcc gcgagaagca gtaccgggaa
     5341 gcggtcgccg cggcggtccg caccctgaac acaactggcg ctgaagaagc gtcgcttcat
     5401 ctcaccgagc tcgccgtcga ccggcgcgac gcggcgtgga aggtggcaca cgcggtgacg
     5461 gtggcgcgcg aatgcgccta ccgcttcacc cggatgaaaa gcaaggacga gaacgcgcag
     5521 atcgcgctgc gccggctcct cttgagcgtc gaccgcgccg ccgtgaaacg cgccaccgcc
     5581 gggctcgagc aggggctcgc cgtcgcgcac ggtgtgggcc tcgcgaagga cctcggcaac
     5641 ctgccgccca atgtctgcac gccgtcctac cttgccgacc aggcacgcga gctggcgaag
     5701 cgctaccgca tgaaggtgac ggtgctcgag cgcgaggaca tggagcgtct cggcatgggc
     5761 accctgctgt cggtcgcgcg cggcagcgcc cagccgccca agctcatcac gctcgagcat
     5821 cgcggcgggg gaaacaggat gaaaccggtc gtgctcgtcg gcaagggcat caccttcgat
     5881 accggaggca tatcgctcaa gcccgcggcc gagatggacg agatgaagtt cgacatgtgc
     5941 ggcgcggcgg ccgtgctcgg cacgatgaag gcggcgggag agatgaggct gccggtcaac
     6001 gtggtcggcg tcatcccggc gaccgagaac atgcccggcg gccgcgccag ccggcccggc
     6061 gacatcgtca aaagcctgtc cggccagacc gtagaaatcc tcaacaccga tgctgaaggc
     6121 cggctcatcc tgtgcgacgc gctgacctat gccgagcgct tcaagcccgc cgcggtgatc
     6181 gacatcgcga cgctcaccgg ggcctgcgtg atcgcgctcg gtcatgtcgc gagcgggctt
     6241 ttcgccaacg acgacgcgct cgcgcgcgag gccgcagccg cgggcgacac cgcatacgac
     6301 agggtatggc gcctgccgct gtgggacgat taccaggaac agttgaagag caacttcgcc
     6361 gacttcgcca acatcggcgg gcgccccgcc ggcgtggtga c
//
LOCUS       DBNGWZ010000485         5130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1951900, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000485 DBNGWZ010000000
VERSION     DBNGWZ010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1951900"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="AC16CX_15015"
     CDS             complement(<1..415)
                     /locus_tag="AC16CX_15015"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR3013852.1"
                     /translation="MESPHPDWIVPDWPAPANVKAFVTTRAGGVSRGAYTSFNLGLRT
                     GDDPRAIAANRERLRAAVPQDPKWLRQMHGCNVVEADTLTGEPEADAAIARRPETVCV
                     ALVADCIPVLVADRAGTTVAIAHAGWRGLARGVIEN"
     gene            complement(418..1362)
                     /gene="rluD"
                     /locus_tag="AC16CX_15020"
     CDS             complement(418..1362)
                     /gene="rluD"
                     /locus_tag="AC16CX_15020"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HIR3013853.1"
                     /translation="MKLTVPAGCAGLRLDQALARLLPEYSRNRLAQWVRSGRIILNGR
                     TAPPRRKVREGEVILVQPVPDPATAVHRAERIPLQVVFEDDVLLVVNKPAGLVVHPGS
                     GNWHGTLLNALLAYAAGLANVPRAGIVHRLDKDTSGLLVVAKTLAAQTDLVRQLQARS
                     VKREYLAVVQGRVARGGKIEAPIGRHPVRRTRMAVTARGRHAVTHYQVLERYAHATLL
                     RCRLETGRTHQIRVHLGALGHPLLGDPVYGRRDGKLVFPRQALHAERIALVHPETRRT
                     ISWQVDPPADMQELISMLRHESARVKHPSPITHRLSRR"
     gene            1409..2215
                     /locus_tag="AC16CX_15025"
     CDS             1409..2215
                     /locus_tag="AC16CX_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR3013854.1"
                     /translation="MALIVRYSLAVLFGVLVAACGLLPDLEDETKGWSAQKLYSEAKS
                     ELNNQNYEQAIKYYQKLEARYPYGRFAQQAQIEIAYAYYKDGEPTQAVAAADRFIKLH
                     PNHPNVDYAYYLKGLANFNDDLGWLGFLGGQDLTERDPKAAREAFDAFKDLVTRFPDS
                     RYAPDSVARMNYLLNALASHEVHVARYYMKRSAYVAAANRVQFALKAYPQAPANEEGL
                     VILVRAYDALGMKDLRDDAERVLIKNFPNSKYLKGDPSKSGAPWWQIWNW"
     gene            complement(2359..3219)
                     /locus_tag="AC16CX_15030"
     CDS             complement(2359..3219)
                     /locus_tag="AC16CX_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3013855.1"
                     /translation="MTALALSLILAAAFVHASWNYFLKRSGGGTVFVWLFASLSALIY
                     APLAVIVLWWEKPDFGWVQFGLMFASAALHTAYYLLLDRGYRSGDLSVVYPLARGTGP
                     LITVLCAVLLLQERPTPVAVAGALLIGGGAIALTGDPRRLRQSGNLPAVGFALLTGCM
                     IASYTLVDKIAVAAWLIPPLVQDWAANLGRVALMTPMALRLKGEIRPTWRRARKEIIA
                     VALLCPLSYILVLTAMVFTPVSYVAPAREVSILVATLMGTQLLAEGDVVQRLTAAAAM
                     VAGIICLAAG"
     gene            complement(3347..>5130)
                     /locus_tag="AC16CX_15035"
     CDS             complement(3347..>5130)
                     /locus_tag="AC16CX_15035"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HIR3013856.1"
                     /translation="ADEYDGPAVVDGDRMSGTRWFPEARLNFAENLLRRRDRSTAIVF
                     WGEDRLKTTVTYAELYAEVSRLSQALQAAGVKAGDRVAGYMPNVPGTVIAMLAATSLG
                     AIWSSCSPDFGVQGVLDRFGQIEPKVLFAADGYFYNGKTIDLVDRLKDIAGNLPSVEK
                     VVVVPYTRPRPPISDVPRAVNVHDLMAPWRPREIRFERFPFNQPLYILYSSGTTGAPK
                     CIVHGAGGTLLQHLKEHQLHVDLKPGERLFYFTTCGWMMWNWLVSALASSAAIVLYDG
                     SPLIGGGRVLFDYADAARISAFGTSAKFIDSTAKAGLKPRLTHRLTALKALLSTGSPL
                     VPESFEYVYGQIKGDLHLASISGGTDIISCFVLGDPTSPVWRGEIQCAGLGMKVEVFD
                     DAGRPVRGEKGELVCTAPFPSMPVSFWNDPDGAKYRAAYFEKFRGVWCHGDYIERTAH
                     DGFVIYGRSDAVLNPGGVRIGTAEIYRQVESLDEVVESLVISQDWPPDHPTDVRVVLF
                     VRLRDGTRLDDALIEKIRRRIRDYATPRHVPACIVQVPDIPRTKSGKIVELAVRNVVH
                     GRPVKNVEALANPEALAHYKDRPELRS"
BASE COUNT          909 a         1639 c         1724 g          858 t
ORIGIN      
        1 cgttctcgat gacccccctc gcaagcccgc gccagccggc atgcgcgatg gccacggtgg
       61 ttcccgcgcg gtcggcgacc agcaccggga tgcaatcggc aacgagcgcg acgcacaccg
      121 tttccggacg gcgcgcgatg gctgcgtcgg cctcgggctc gcccgtcagc gtgtcggcct
      181 cgacaacgtt gcagccgtgc atctggcgca gccatttggg atcttgcggc acagcggcac
      241 gcagccgctc ccggttggcg gcgatcgccc gcggatcatc gcccgtgcgc agtccgagat
      301 tgaagctggt gtatgcgccc cggctgacgc cgccggcccg cgtggtgacg aacgccttga
      361 cgttggccgg cgccggccag tcggggacga tccagtcggg atgcggtgat tccatcttca
      421 acggcgtgat aagcgatgag tgatgggtga tgggtgcttc actcgcgcgc tttcgtggcg
      481 cagcatcgag atcagttcct gcatgtcggc gggcgggtcg acctgccagc ttatcgtgcg
      541 gcgggtttcg gggtgcacca gtgcgatccg ctcggcgtgc agggcctgcc gcggaaagac
      601 gagcttgccg tcgcgcctgc cgtagacggg gtcgccgaga agcgggtgac ccagtgcgcc
      661 gaggtgcacc cggatctgat gggtgcgtcc ggtttcgagc cggcagcgca acagggtggc
      721 atgtgcatag cgctcgagca cctggtagtg ggtcaccgcg tgccgtccgc gcgccgtcac
      781 tgccatccgg gtgcgcctga ccggatggcg tccgatgggg gcctcgatct tgccgccgcg
      841 cgcaacgcgg ccctgcacga cggcgagata ttcgcgcttg acgctgcgcg cctggagctg
      901 gcgcacgaga tcggtctgcg cggcgagcgt tttggccacc accagcagac cgctggtgtc
      961 cttatcgaga cgatgcacga ttcccgctcg ggggacgttg gcgagccccg cagcatacgc
     1021 gagcagggcg ttgagcaacg tgccatgcca gttgccgctg ccgggatgca cgacgagccc
     1081 cgccggcttg ttgacgacca gcaggacatc gtcctcgaac acgacctgga gcgggatgcg
     1141 ctccgcgcgg tgcacggcgg tcgcggggtc gggaaccggc tggaccagga tcacttcacc
     1201 ttcccgtact tttcgcctgg gaggagcggt gcggccgttg aggatgatcc ggccgctacg
     1261 cacccattgc gcgagccggt tgcgcgagta ttccggcaac aaacgcgcaa gcgcctggtc
     1321 caatcgcaga ccggcgcatc cggcgggcac ggtgagcttc acgggcctcg ttcccgtgcc
     1381 ggttgcgtta taattttcaa ggggttctat ggcgctcatt gttcgatata gtttagcggt
     1441 actgttcggc gtgctggttg ccgcgtgcgg gctgctgccc gatctggagg acgagaccaa
     1501 gggctggtcg gcgcaaaagc tctacagcga agccaagagc gagctcaaca accagaacta
     1561 cgagcaggcg atcaagtatt accagaagct cgaggcgcgc tatccctacg ggcgctttgc
     1621 ccagcaggcg cagatcgaga tcgcctacgc ctattacaag gacggcgagc cgacgcaggc
     1681 cgtggcggca gccgaccgct tcatcaagct gcaccccaat catccgaacg tcgactacgc
     1741 ctattacctg aaggggctgg ccaatttcaa cgacgacctg gggtggctgg gcttcctcgg
     1801 cggacaggat ctcaccgaac gcgatcccaa ggcggcgcgc gaagcgttcg atgccttcaa
     1861 ggacctggtg acgcgttttc cggatagccg ttacgcgccg gattccgtcg cccgcatgaa
     1921 ttacctgctg aatgcgctcg cttcacacga ggttcacgtt gcgcgctatt acatgaagcg
     1981 cagcgcctat gtcgcggcgg ccaaccgcgt ccagttcgcg ctcaaggctt acccccaggc
     2041 accggccaac gaagaggggc tcgtgatcct ggtgagggcc tacgatgccc tgggcatgaa
     2101 ggacctgcgc gatgacgccg agcgcgttct gataaagaac tttccgaaca gcaaatactt
     2161 gaagggcgat ccgagtaagt ccggcgctcc gtggtggcag atctggaact ggtgatcgcc
     2221 cgcgatcacc cgtttcaagc ttcaagttcc aaggtacaag tcacaaggta cagggcacaa
     2281 ggcgttgaac tgcttgtgcc ttgttgcttg tagattgctg cttgtatctt gtaacttgca
     2341 tcttgagccg taggcggtct atcccgctgc aaggcaaatg attccggcca ccatcgcagc
     2401 ggcggccgtc agacgctgca caacgtcgcc ctcggccagc agctgcgtcc ccatcaacgt
     2461 cgcgaccaga atgctgacct cccgcgccgg cgcgacgtag ctcaccggcg tgaacaccat
     2521 cgcagtgagc accaggatgt aggacagcgg gcagagcagc gcgaccgcga tgatttcctt
     2581 ccgggcgcgc cgccaggtcg ggcggatctc gcccttaagc ctgagcgcca ttggcgtcat
     2641 cagggccacg cgcccgagat tggccgccca gtcctgcacc agcggtggga tgagccaggc
     2701 ggcgaccgca atcttgtcca ccagcgtata gctggcgatc atgcagccgg tgagcagcgc
     2761 gaagccgacc gccgggagat tgccggactg cctgagcctg cgcggatcgc ccgtcagcgc
     2821 gatcgcgccg ccgccgatga gcagcgcccc ggccacggcg accggagtcg ggcgctcctg
     2881 caataacagc accgcgcaca gcacggtgat gagcgggccg gtgccgcgcg ccaggggata
     2941 gaccaccgag agatcgccgc tgcggtagcc gcgatcgagc aggaggtaat aggcggtgtg
     3001 cagcgccgcg ctcgcgaaca tgaggccgaa ttgcacccat ccgaaatcgg gtttttccca
     3061 ccacaggacg attacggcaa gcggggcgta gatcagcgcg gagaggctgg cgaacagcca
     3121 gacgaacacg gtgccgccgc cggagcgctt gaggaagtag ttccagctcg catggacgaa
     3181 ggcggccgcg aggatcagcg acagggcgag ggcggtcatg aaaggatgag ggaagaggga
     3241 ttagggatga agaatgaccc ccgcagtgcg gaagcaaatg ccaggtcagc tttcaacctt
     3301 cacgcgaagc cgcacagctc atccttcatc cttccgcctt catccttcag gatcgcagtt
     3361 ctgggcggtc cttgtagtgc gcaagcgcct ccggattcgc gagcgcttcc acgttcttca
     3421 ccggccggcc atgcaccacg ttgcgcaccg ccagctccac gatcttgccg ctcttggtgc
     3481 ggggaatatc ggggacctgc acgatgcacg ccggaacgtg gcgcggagtg gcgtagtcgc
     3541 ggatcctgcg cctgattttc tcgatgagcg cgtcgtcgag ccgcgtcccg tcgcgcaggc
     3601 gcacgaacag caccacgcgc acgtcggtgg gatggtcggg aggccagtcc tggctgatga
     3661 cgaggctttc caccacctca tccaggctct cgacctggcg gtaaatctcc gcggtgccga
     3721 tgcgcacgcc cccgggattc agcacggcgt cggagcgccc gtagatcacg aacccgtcgt
     3781 gcgccgtccg ctcgatgtag tcgccatggc accacacgcc ccgaaatttt tcgaagtagg
     3841 cggcgcggta cttggcgccg tcggggtcgt tccagaagct gaccggcatc gagggaaagg
     3901 gcgcggtgca gacgagctcc cccttctcgc cccgcaccgg ccggcccgcg tcgtcgaaca
     3961 cctcgacctt catgccgagc ccggcgcatt gaatctcgcc gcgccacacg ggggacgtgg
     4021 gatcgccgag cacgaagcac gagatgatgt cggtgccgcc cgagatcgag gctaggtgaa
     4081 ggtcgccctt gatctggcca tagacgtatt caaagctctc cggcacgagc ggggagccgg
     4141 tcgagagcag ggcctttagc gccgtgagcc ggtgggtgag gcgcggcttg aggcccgcct
     4201 tggccgtgct gtctatgaac ttcgcagagg tgccgaaggc gctgatgcgc gcggcgtccg
     4261 catagtcgaa cagcacgcgt ccgccgccga tcagcggcga gccgtcgtag aggacgatgg
     4321 cggcgctcga ggcgagggcg gataccagcc agttccacat catccagcca caggtcgtga
     4381 agtagaacag gcgctcgccc ggcttgagat cgacgtgcag ctggtgctcc ttcaggtgct
     4441 gaagcaaagt gccgccggcg ccgtgcacga tgcacttcgg cgcgccggtg gtcccggagg
     4501 agtaaaggat gtagagcggc tgattgaacg ggaagcgctc gaagcggatt tcgcgcgggc
     4561 gccagggcgc catgaggtcg tggacgttca ccgcccgggg aacgtctgaa atcggcggtc
     4621 gcggccgcgt atagggcacg acgacgactt tttccacgga gggaagattt ccggcgatgt
     4681 ccttgagacg atccaccagg tcgatcgtct tgccgttgta gaaataaccg tcggcggcga
     4741 acagcacctt gggctcgatc tggccgaagc ggtcgagcac gccctggacg ccgaaatcgg
     4801 gagagcacga ggaccagatc gcaccgaggc tggttgccgc cagcatcgcg atcaccgtgc
     4861 ccggcacgtt cggcatgtag cccgcgaccc ggtcacccgc cttgacgccg gcggcctgca
     4921 acgcctgcga gaggcgcgat acctcggcgt agagttcggc gtaggtgacg gtcgttttca
     4981 gccggtcctc gccccagaac acgatggcgg tggagcggtc gcggcgccgc agcaggtttt
     5041 cggcgaagtt gagccgtgcc tcgggaaacc agcgcgtccc ggacatcctg tccccgtcca
     5101 ccaccgccgg cccgtcgtac tcgtcggcga
//
LOCUS       DBNGWZ010000486         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1575885, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000486 DBNGWZ010000000
VERSION     DBNGWZ010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1575885"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC16CX_15040"
     CDS             complement(<1..917)
                     /locus_tag="AC16CX_15040"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013857.1"
                     /translation="MNKKTQAIDTPAAHLLGFARSLETQAIPRDVLERARWCLLDGLG
                     CGLFGAGQSWSRIMFDEMSAERSQGPCTVFGQRGTLPAAPAAMCNGTAIHGFELDDVM
                     AAALVHPGTVIVPAILAAAESVDASGEDLLRGIVVGYEAMSRLSLALGTGPSERGFHK
                     TSVVGPVAAAIAAGAVMRLPYDQLTCAVGLACSTGSGVKSFAAGHGGGMVKRMHAGRA
                     AEAGVRVAQLARRGFTGPASAIDGRMGLLEAFSGASAAPEHLQRGLGEEWALRDVWIK
                     VYPVCGWIQGPAQLLLELRGTQPLPEKRI"
     gene            complement(988..1974)
                     /locus_tag="AC16CX_15045"
     CDS             complement(988..1974)
                     /locus_tag="AC16CX_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013858.1"
                     /translation="MKDHGAQRFTSALGLATLAGACFLWSTANFAEDFPNRPARFISA
                     MAPGGSGDLNARRLADALRNRLGQPIVVENRSGAGGSIAAVSAAGATPDGYTLFFASD
                     QIFTVNPILQKNLPFSLDDFQPLALVSRAPHILLVSAVPAKNLAELVAYAKTRPAGLN
                     FGSGGVSTSPHLAGELFKGLAGIRLVHVPYKGAAPSLTALLGNEIQLLFDSTLTSIGH
                     IRSGRARGLAIASLTRSGLLPDLPTFDESGFRGFQAGVTHGLLLPAKTPRARVALLSK
                     LVNEVLNEPAYRKSMTDLGAQVLGGTPERFRAHLLDERKKWEIVIKRENIHL"
BASE COUNT          364 a          751 c          734 g          344 t
ORIGIN      
        1 ctgatgcgct tctcgggcag gggttgcgtg ccgcgcagtt cgaggagcag ctgcgccggc
       61 ccctggatcc agccgcacac gggatagacc ttgatccaca cgtcccgcag cgcccactcc
      121 tcgcccaagc cgcgctgcag atgctcgggg gcggcggacg cgccgctgaa cgcttccagc
      181 aggcccatcc ggccgtcgat cgccgatgcg gggccggtaa agccccggcg ggcgagctgg
      241 gccacgcgca cgccggcttc ggcggctcgg ccggcgtgca tgcgcttcac catgcccccg
      301 ccgtggccgg cggcgaagct cttgacgccc gacccggtgg agcacgcaag gccgacggcg
      361 caggtgagct ggtcgtaggg cagcctcatc accgcgcccg ccgcgatggc agccgcgaca
      421 gggcccacca cgctggtctt gtgaaatccg cgctcggaag gaccggttcc cagcgcgagg
      481 ctcagccgcg acatggcctc gtagccgacg acgatgccgc gcagcaggtc ctctccggag
      541 gcgtcgacgg attccgccgc cgccaggatc gccgggacga tcaccgtgcc gggatgaacc
      601 agcgcggcag ccatgacgtc gtcgagctcg aatccgtgta tggccgtgcc gttgcacatc
      661 gcggccggtg ccgcaggcaa cgttccgcgc tggccgaaga cggtgcacgg accctgcgac
      721 cgctcggcgg acatctcgtc gaacatgatc ctggaccacg actgtcccgc cccgaacagg
      781 ccgcagccca gcccgtcaag caggcaccac ctcgcgcgct ccagaacgtc gcgcggtatc
      841 gcctgcgttt ccagcgaccg tgcaaacccc agcaagtgcg cggccggcgt gtcaattgcc
      901 tgtgtcttct tgttcattgc gtcacgggcc cacagtcgcc gcacgcccga aacaatacac
      961 ggtcgcggtc cgtccgcccc cgggggtcta gagatgtatg ttttcccgct tgatgacgat
     1021 ctcccatttc ttgcgctcgt cgagcaggtg ggcgcgaaag cgctccggcg tcccgccgag
     1081 cacctgtgcg ccgagatcgg tcatcgactt gcgatacgcc ggctcgttca ggacttcgtt
     1141 gacgagcttg ctgagcagcg cgacccgggc ccggggcgtc ttggcgggca gcagcagccc
     1201 gtgcgtgacg ccggcctgga atccccggaa accgctctcg tcaaacgtcg gcagatcggg
     1261 gagcagcccg gaacgcgtca ggctggcaat ggccaatccc cgcgcgcgac cgctgcggat
     1321 gtgaccgatg gaggtgaggg tgctgtcgaa cagaagctgg atttcattgc cgagcagggc
     1381 ggtcagggaa ggcgccgcgc ccttgtaggg cacgtgcacg agcctgatcc ctgccaggcc
     1441 cttgaacagc tccccggcaa gatgagggga cgtgctcacc ccgccggaac cgaaattgag
     1501 gcccgcaggc cgggtctttg cataggcgac gagttccgca aggtttttcg cgggcaccgc
     1561 gctcaccagc aggatgtgcg gggcgcggct gaccagggcg agcggctgga agtcgtcaag
     1621 gctgaacggc aggttcttct gcaggatcgg gttgaccgtg aaaatctggt cggacgcgaa
     1681 aaacagcgtg tatccgtcgg gcgtggctcc cgccgccgag acggcggcta tgctgccccc
     1741 ggcgccggat ctgttctcga ccacgatggg ctggcccagc cggttcctga gcgcgtcggc
     1801 caggcggcgt gcgttgagat ccccgctgcc gccgggcgcc atcgccgaaa tgaagcgggc
     1861 aggcctgttc gggaaatcct ccgcgaaatt cgccgtggac cacaggaaac acgctccggc
     1921 gagcgtggcc aggccaaggg ctgaggtgaa acgctgcgcg ccatggtcct tcatgaatga
     1981 attctcctca aatgtagaaa tttgagcgcc atcgccggat ccgcctcagg cggacaactc
     2041 gagtatgcgg gaatagaccc ggtcgtccaa ttgaatcacg ccggccgcat gtttgcgatg
     2101 ctgcatgctg ccgtcgcccg gagcccttac cttcaccgtg ccgggcaccg gccgcgtcga
     2161 gttgattacc cgcctcagct cggacacccg gga
//
LOCUS       DBNGWZ010000487         9503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1195533, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000487 DBNGWZ010000000
VERSION     DBNGWZ010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1195533"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16CX_15050"
     CDS             complement(<1..344)
                     /locus_tag="AC16CX_15050"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR3013859.1"
                     /translation="MKYSFEDRKVVCKGDYWIAPNATVIGSVVLEPNASVWFGCIVRG
                     DDDTITIGENSQLQDGCVLHVDAGFPITIGKNVSVGHMAMLHGCTIGDGTLIGIKAVI
                     LNGAKVGRNCLIG"
     gene            complement(446..2518)
                     /locus_tag="AC16CX_15055"
     CDS             complement(446..2518)
                     /locus_tag="AC16CX_15055"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3013860.1"
                     /translation="MSYGLLFALICAIVALVYGAVSIKWVLGQPAGNERMREIAAAVQ
                     QGATAYLWRQYKTISIVGVILFFIIGFGLDSWATAVGFLIGAVLSGAAGVIGMNISVR
                     ANVRTAEAARKGLNEALSLAFRGGAITGLLVVGLGLLGVTGFYWVLLESAAHGAPVKK
                     ILEPLVGLAFGGSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYAVTIIATMLLGALLLSKPAVAPLAVAYPLALGGVSIIAS
                     IIGCFFVKATPGSKIMNALYKGVIISGVIAAIGFVFVTYWMMGGIAEQYPELTPLRLL
                     GAALVGLVLTALMMVITEYYTATEFSPVRHIAAASTTGHATNIIAGLGVSMKATAWPV
                     IAVCASIYAAYQMAGIYGLAIAATAMLSMTGMIVALDAYGPITDNAGGIAEMAHLPKE
                     VRAITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALEAAGKAVDFSLADPAV
                     VIGLFIGGLVPYLFGAMAMEAVGRAAGSVVVEVRRQFKEIKGIMEGTAKPDYSRAVDM
                     LTRAAIKEMMVPSLLPVLVPIVVAFGMNWLMGPGAGVKALGGVLMGTIVTGLFVAISM
                     TTGGGAWDNAKKYIEDGNHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIV
                     ALLIVPLL"
     gene            complement(2648..2869)
                     /locus_tag="AC16CX_15060"
     CDS             complement(2648..2869)
                     /locus_tag="AC16CX_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013861.1"
                     /translation="MTLDRARELLKVQAGFGGGYNANSSKLILSEVMREHCQEAVDRL
                     IVELELDRIFGFQRGTRFEGGVAHAPKEP"
     gene            complement(2959..4221)
                     /locus_tag="AC16CX_15065"
     CDS             complement(2959..4221)
                     /locus_tag="AC16CX_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3013862.1"
                     /translation="MSRKNAFYAQSGGVTATINTTACAVIETARKHRNRIGKVYAGRD
                     GIIGALTEDLIDTSKESAAAIRALRHTPGGAFGSCRYKLKGLEESRAQYERLIEVFKA
                     HDIGYFLYNGGNDSADTSYKVSQVAQRLGYPLICVGIPKTVDNDLALTDCCPGFGSVA
                     KYVATSIREAGYDVASMARTSTRVFILEVMGRHAGWITAACGLAQEKEGDAPHVLLFP
                     EIPFDQEKFLSKVDETIRKYGYCTVGVSEGVRWPDGKFLSETGLRDAFGHAQLGGVAP
                     FIAQLVQDKLKQKYHWAVADYLQRSARHIASKTDALQSYAVGKAAVELAVKGRSGVMP
                     IIVRKSGKPYRWTIGVAELKDIANAEKMMPRDFVTEDGFHITAKCRQYLEPLVQGEDY
                     PPYKNGLPAYVKLRNAAVPKKLGTPFKI"
     gene            complement(4310..4453)
                     /locus_tag="AC16CX_15070"
     CDS             complement(4310..4453)
                     /locus_tag="AC16CX_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013863.1"
                     /translation="MTEQYGTGHDFDDIADRVVTTLRLQADHEKDRAGATSGKTGADL
                     DNP"
     gene            complement(4450..5115)
                     /gene="adk"
                     /locus_tag="AC16CX_15075"
     CDS             complement(4450..5115)
                     /gene="adk"
                     /locus_tag="AC16CX_15075"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - AMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3013864.1"
                     /translation="MRIILLGMPGSGKGTQAQFLMDRYEIPQVSTGDMLRAAIKAGTP
                     LGLKAKGHMDRGELVPDDVVIELVKQRIKEKDCANGFILDGFPRTIQQADALRNAGVG
                     IDYVIEIEVSDEEILRRMSGRRMHPASGRTYHVEFNPPKVPGKDDATGEPLIQRPDDN
                     EETVKKRIATYHAQTKPLIDYYFKWAVSRDRRAPRYVNIYGKGSVEHIRDKMFAAVRD
                     DIR"
     gene            complement(5367..6182)
                     /gene="kdsB"
                     /locus_tag="AC16CX_15080"
     CDS             complement(5367..6182)
                     /gene="kdsB"
                     /locus_tag="AC16CX_15080"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR3013865.1"
                     /translation="MENPLTTTFRSRALPISRSHVKFTVIIPARYASSRFPGKPLADI
                     KGKPMVVRVAERARRSGAAEVLIATDHAGIAEAAERHGFEAVMTRARHATGTDRIAEV
                     AARRRYSPARIIVNVQGDEPLIEPSLIRRVAVGLARHDDAAMASACHPISNARDLANP
                     NVVKVVLDNDGYALYFSRAAVPYARDAFRRGIARVPRGLPVYRHLGIYAYRAAFLRKF
                     ARLEPVAIERYEALEQLRALAHGYRIAVCVTHRAPHPGVDTPADLRRVRRHFA"
     gene            complement(6215..6397)
                     /locus_tag="AC16CX_15085"
     CDS             complement(6215..6397)
                     /locus_tag="AC16CX_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR3013866.1"
                     /translation="MDSKLLDILVCPLCKGPLSYKKPAAELICKPCRLGYPIKDDIPV
                     MLEDEARKLPPEEEVS"
     gene            complement(6404..7414)
                     /gene="lpxK"
                     /locus_tag="AC16CX_15090"
     CDS             complement(6404..7414)
                     /gene="lpxK"
                     /locus_tag="AC16CX_15090"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR3013867.1"
                     /translation="MVWIEQHWERVTPVSALLYPLSLAYGAAVGIRRAAYRSGIAGTT
                     RLPVPVIVVGNLTVGGAGKTPLTLWLAEFLSARGRTPGIVCRGYGGRSRAPRRVLSDS
                     DPFACGDEAVLLARRSGCEVWTGADRIAAARSLLAAQPACDVVVSDDGLQHYALARDI
                     EICVVDAARGFGNGWLLPAGPLREPPSRLAGVDAVVINGEGASHPSVARLPGGAARIA
                     MKLEGREFRNLLNPGRLVGTEFFRGKRVHAVAGIGNPQRFFSHLQELGLDFVAHPFPD
                     HHPFTASDLVYAGAEAVLMTEKDAVKCRRLASEIQWELAVDAVPDPALSELVLRKLGM
                     TG"
     gene            complement(7514..7933)
                     /locus_tag="AC16CX_15095"
     CDS             complement(7514..7933)
                     /locus_tag="AC16CX_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR3013868.1"
                     /translation="MRFRPRAFREEPEINFIPMIDVLLVILIFLMVTTTYSRYAELQI
                     NLPTADAEHPPERPDQIDVAVDPQGRYSINRNPVGFSGAAAFSAELRRAAGNMKEPVV
                     VISADANAPHQSVINVMEAARIAGYGKITFTTQAGKK"
     gene            complement(7930..8538)
                     /locus_tag="AC16CX_15100"
     CDS             complement(7930..8538)
                     /locus_tag="AC16CX_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR3013869.1"
                     /translation="MFAIIEAAGWPIWFLIIASVIALAIIGERWWSLREAVVMPRNLL
                     AQVVQDYRQNGVTPQMLSRLANNSPLGQVFAAGLKNTKSPLPIIKEAIEESGRTVAIE
                     LERFLTSLGTIAVIAPLLGLFGTVVGMIEIFGAQTPTGTNPLVLAHGISIALYNTAYG
                     LVVAVPAMIFYRYFRAKVDEMLVDMEMQAIKLVEIIHGERQV"
     gene            9046..>9503
                     /gene="xseA"
                     /locus_tag="AC16CX_15105"
     CDS             9046..>9503
                     /gene="xseA"
                     /locus_tag="AC16CX_15105"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3013870.1"
                     /translation="MPLAYPELPPVISVAELNRIARDLLERNLPLLWVAGEVSNFKRY
                     DSGHCYFTLKDAQAQVDCVMYRHRAQLLGWAPQDGMKVEVRALPTLYEARGRFQLNVE
                     VVRRAGLGALYEAFERLKARLEKEGLFDLARKRPLPRFPRAVGVVTSPQAA"
BASE COUNT         1675 a         3062 c         3085 g         1681 t
ORIGIN      
        1 ccgatcaggc agttcctgcc gactttcgcg ccgttcaaga tcaccgcctt gatgccgatc
       61 agcgtcccgt cgccgatggt gcagccgtgc agcatcgcca tgtggccgac actcacgttc
      121 ttcccgatgg tgatggggaa gccggcgtcc acgtgcagca cgcagccgtc ctgcagctgc
      181 gaattctcgc cgatggtgat ggtgtcgtcg tcgccgcgca cgatgcaccc gaaccacacg
      241 ctggcgttcg gctcgagcac caccgagccg atcacggtcg cgttcggagc gatccagtag
      301 tcgcccttgc atacgacctt gcggtcctcg aaagagtatt tcatgtcggc tgtcagcgta
      361 cagctatcag cggtcagcgt aaatcgttca atgggtttcg ctgacggctg aaagctgagc
      421 gctgaatgct tgcgatcgtg ccgcactaca gcagcggcac gatgagcaac gcgacgatgt
      481 tgatgatctt gatgagcgga ttgaccgccg ggccggcggt gtccttgtag ggatcgccga
      541 ccgtgtcgcc ggtcaccgcc gccttgtgcg cgtcggagcc cttgccgccg tggttgccgt
      601 cctcgatgta cttcttggcg ttgtcccagg cgccgccgcc ggtggtcatc gagattgcga
      661 cgaaaagacc ggtgacgatc gtgcccatca gcacgccgcc gagcgccttc acgcccgcgc
      721 ccggccccat cagccagttc atgccgaacg ccaccacgat cggcaccagc accggcagca
      781 gcgacggcac catcatctcc ttgatcgcgg cgcgcgtgag catgtccacc gcgcgcgagt
      841 aatcgggctt ggcggttcct tccatgatgc ccttgatctc cttgaactgc cggcgtacct
      901 cgaccacgac cgagcccgcc gcgcggccga cggcttccat cgccatcgcg ccgaagaggt
      961 agggcacgag gccgccgata aagagcccga tgaccaccgc cggatcggcg aggctgaaat
     1021 ccaccgcctt gcccgccgcc tcgagcgcgt gggtgtaatc ggcgaacagc accagcgccg
     1081 cgagacccgc cgagccgatg gcgtagccct tggtcaccgc cttggtcgtg ttgcccaccg
     1141 catcgagcgg atcggtgatg gcgcgcacct ccttcggcag atgcgccatc tcggcgatgc
     1201 cgcccgcgtt gtcggtgatc gggccgtaag cgtcgagcgc gacgatcatg ccggtcatcg
     1261 agagcatcgc ggttgcggcg atcgcgagcc cgtagatccc ggccatctgg tacgcggcgt
     1321 aaatgctcgc gcacaccgcg atcaccggcc atgcggtcgc cttcatcgaa acgccgaggc
     1381 ccgcgatgat gttggtcgcg tggcccgtcg tggaggccgc ggcgatgtgg cgcaccgggg
     1441 agaactcggt cgcggtgtag tactcggtga tcaccatcat gagggcggtg aggaccaggc
     1501 ccaccagcgc ggcgccaagg agcctcagcg gcgtcagctc tggatactgc tcggcaatgc
     1561 cgcccatcat ccagtaggtg acgaagacga agccgattgc ggcgatgacg cccgagatga
     1621 tcacgccttt gtagagcgcg ttcatgatct tgctccccgg cgtggccttg acgaagaagc
     1681 agccgatgat ggaggcgatg atcgagacac cgcccagcgc gagaggatag gccacggcga
     1741 ggggggcgac cgccggtttc gacagcagca gcgcgccgag cagcatggtg gcgatgatgg
     1801 tcacggcgta ggtctcgaac aggtccgccg ccatgccggc gcagtcgccg acgttatcgc
     1861 ccacgttgtc ggcgatcaca gcggggttgc gcggatcgtc ctcggggatg ccggcctcca
     1921 ccttgcccac taggtcggcg ccgacatccg cccctttggt gaagatgccg ccgccgagcc
     1981 gcgcaaagat cgagatcagc gagccgccga aggcgagccc cacgagcggc tcgaggattt
     2041 tcttgacggg cgccccgtga gcggcggact cgagcaacac ccagtagaac ccggtgacgc
     2101 cgagcagccc gagcccgacg accagcaatc cggtgatggc gccgccgcgg aacgcgaggg
     2161 agagcgcctc gttgagtccc ttgcgggcgg cttccgcagt gcgcacgttg gcccgcaccg
     2221 agatgttcat gccgatgacg ccggccgccc ccgaaagcac cgcgccgatc aggaagccga
     2281 ccgccgtggc ccaggagtcc agaccgaaac cgatgatgaa gaacaggatg acgccgacga
     2341 tgctgatcgt cttgtactgg cgccacaggt aggcggtggc accctgctgc acggcggcgg
     2401 cgatctcgcg catgcgctcg ttgcccgcgg gctggccgag gacccacttg atcgagaccg
     2461 cgccgtaaac gagtgcgacg atcgcgcaga tcagcgcaaa cagcaatcca tatgacatga
     2521 caactccctg ttattgatag cgttcagcgg tcagctcgca gccgtcagct tctcggacgc
     2581 aagtttcaag ctgatcgctg aaagctgacg gctgaccgct agtttaattt ctgttcccgc
     2641 cgctctctta cggctccttt ggggcgtgcg caacgccgcc ctcgaagcgc gtgccgcgct
     2701 ggaacccgaa tatccggtca agctcgagct cgacgatcaa ccggtcaacc gcctcctggc
     2761 agtgctcgcg catgacttct gaaaggatca gcttggagga gttggcgttg tagccgccgc
     2821 caaagccggc ctgcactttc agcagctcgc gagctctatc cagagtcatt gctcgccggt
     2881 gaggggtaaa gtgtgagagg tgaggcgcaa ccccgatcga atccagtcag gtctttcgct
     2941 cctcactcct cacgccgttc agatcttgaa gggcgtcccg agcttcttcg gcaccgccgc
     3001 gttcctgagc ttcacatagg ccggcagccc gttcttgtac ggcggatagt cctcgccctg
     3061 gaccaggggc tcgaggtatt gccggcactt tgcggtgatg tgaaagccgt cctcggtgac
     3121 gaagtcgcgc ggcatcatct tctccgcgtt ggcgatgtcc ttgagctccg ccacgccgat
     3181 cgtccagcga tacggcttgc ccgacttcct gacgatgatc ggcatcacgc cgctgcggcc
     3241 cttcacggca agctccaccg cggccttgcc caccgcgtag gactgcaggg cgtcggtctt
     3301 cgaggcgatg tggcgcgcgg aacgctgcag gtagtcggcc accgcccaat ggtatttctg
     3361 cttgagcttg tcctggacca gttgcgcgat gaacggcgcg acgccgccca gctgggcgtg
     3421 gccgaacgcg tcccgcagcc ccgtttccga caggaacttg ccgtcgggcc agcgcacgcc
     3481 ctcggaaacg ccgacggtgc aatagccgta cttgcgaatg gtctcgtcca ccttcgacag
     3541 gaatttttcc tggtcgaacg ggatttccgg gaacagcagg acgtgggggg cgtcgccctc
     3601 cttttcctgt gcgagcccgc acgccgccgt gatccagccc gcgtgccgcc ccatcacttc
     3661 caggatgaag acgcgggtcg aagtgcgcgc catggaggcg acgtcatagc ccgcctcgcg
     3721 gatgctggtc gccacgtact tggcgaccga gccgaagccg gggcagcagt cggtgagcgc
     3781 gaggtcgttg tccacggtct tggggatgcc gacacagatc agggggtagc ccagcctctg
     3841 cgccacctgt gaaaccttgt acgaagtgtc cgccgaatcg ttgccgccgt tgtagaggaa
     3901 atagccgatg tcgtgcgcct tgaacacctc gatcagccgt tcgtactggg cgcggctttc
     3961 ctccagcccc ttcagtttgt agcggcagga gccgaaggcg ccgcccggcg tgtgccgcag
     4021 cgcgcggatc gcggccgccg attccttcga agtgtcgatg agatcctcgg tcagcgcgcc
     4081 gatgatgcca tcgcgcccgg cgtagacctt gccgatccgg ttcctgtgct tgcgcgcggt
     4141 ctcgatgacg gcgcaggcgg tggtgttgat ggtggcggtc acgcccccgg actgggcgta
     4201 aaaggcgttc ttcctggaca tacggctaca atgcggtcac aaagatgacg gagaaaaccg
     4261 gaaattcgag aaaggcgaat tttaccactc accactcacg actcaccatt cacggattat
     4321 caagatcggc gccggtcttg ccggaagtcg cgccggcgcg gtccttctcg tgatcggcct
     4381 gcagccgcag ggtggtgacc acgcggtcgg cgatgtcgtc gaaatcgtgc ccggtgccgt
     4441 actgttccgt caacgaatat cgtcgcgcac cgcggcgaac atcttgtcgc ggatgtgctc
     4501 gaccgacccc ttgccgtaga tgttgacgta acggggcgcg cggcggtcgc ggctcaccgc
     4561 ccacttgaag taataatcga tcagcggctt ggtctgcgcg tggtaggtcg caatgcgttt
     4621 cttgacggtt tcctcgttgt catcagggcg ctggatgagg ggctcccccg tggcgtcgtc
     4681 cttgccgggt accttgggag gattgaactc aacgtgatag gtgcggcctg aggcgggatg
     4741 catgcgtcgt ccgctcatgc gccgcaggat ctcttcgtcg ctcacctcga tctcgatcac
     4801 gtaatcgatc ccgacacccg cgttacgcaa ggcatccgcc tgctgaatgg tgcgggggaa
     4861 gccgtccagg atgaagccgt tggcgcagtc cttctccttg atgcgctgct tcacgagctc
     4921 gatcacgacg tcgtcgggaa cgagctcgcc ccggtccatg tggcctttcg ctttcaggcc
     4981 gagcggcgtg ccggccttga tcgccgcgcg cagcatgtcc ccggtcgaga cctgcgggat
     5041 ttcataccgg tccatcagaa attgggcctg cgtgcctttg cccgagccgg gcatgcccag
     5101 cagaatgatc ctcattttcc tcccctcccg ttgttctcgt tcactgtatc acccaatcgg
     5161 cgcactcaca acgaggacgc cgcggcaggc cttgccgttg tcatgggagc gcccgactgc
     5221 ggtaggcggt ttgcagtggg cagtggacag cgtgcagatc gcaaagccag ttgcaaggta
     5281 caagtcacaa gcagcaagtt acaaaggtcg tgtcgtcagc tgtagctgct tgaaacttgt
     5341 agctcaacac ttaccgcctg ccgaccctaa gcaaagtgac gacggacgcg gcgcagatcg
     5401 gctggcgtat cgacgccggg atgcggggca cggtgcgtca cgcataccgc gatgcggtag
     5461 ccgtgggcga gcgcccgcaa ctgctcgagc gcctcgtaac gctcgatcgc aaccggctcg
     5521 agccgcgcga acttccgcag aaacgcggcg cggtacgcgt agatgcccag gtggcggtag
     5581 accggaaggc cgcgcggcac ccgggcgatc ccgcgcctga aggcgtcgcg cgcataagga
     5641 acggcagcgc ggctgaaata gagcgcatag ccgtcgttgt cgagcaccac cttcacgacg
     5701 ttcggattgg cgaggtcccg cgcgttcgat atcgggtggc aggcgctcgc catggccgcg
     5761 tcgtcgtggc gggcgagacc gacggcaacc cgccggatca gcgacggctc gatgagcggc
     5821 tcgtcgccct gcacgttgac gatgatgcgt gccggggaat agcggcgccg ggcggcgacc
     5881 tcggcgatgc ggtcggtgcc ggtcgcatgc cgggcccgcg tcatgaccgc ctcgaacccg
     5941 tggcgctcgg cggcttccgc aattcccgcg tggtcggtgg cgatcagcac ttccgctgca
     6001 ccgcttcgac gcgcgcgctc cgccacccgc accaccatcg gcttgccctt gatgtcggcg
     6061 agcggcttgc cgggaaagcg cgaggaggcg tagcgggcgg gaataataac ggtgaacttc
     6121 acgtgagagc gggagatcgg gagggcgcga gatcgaaagg tcgtagtaag cgggttttcc
     6181 atttcacgct ctcccgccct cacgcgctcc cgattcagga aacttcctcc tctggaggaa
     6241 gtttgcgggc ctcatcctcg agcatcaccg ggatgtcgtc cttgatcggg tagccgaggc
     6301 ggcagggttt gcagatgagc tcggcggcgg gcttcttgta cgagagagga cccttgcaaa
     6361 gcgggcacac caggatgtcg agcagcttgc tgtccatttc aactcaaccg gtcattccca
     6421 gcttgcgcag cacaagttcg ctcaacgcgg gatcgggcac tgcgtccacc gccagctccc
     6481 attgtatttc gctggcgagc cgcctgcatt ttaccgcatc cttttccgtc atcagcactg
     6541 cttccgcccc ggcgtacacg aggtcggaag cggtgaacgg gtgatgatca gggaaggggt
     6601 gcgcgacaaa gtcgagaccc aattcctgaa gatgcgagaa aaagcgctgg ggattgccga
     6661 tcccggccac ggcatgcacg cgctttccgc gaaaaaactc ggtgccgacg agccgtccgg
     6721 gattgagcaa attgcggaac tcccggcctt ccagcttcat ggcgatccgg gccgcgccgc
     6781 ccggcaggcg ggcgaccgag ggatgggacg caccctcgcc gttgatgacc accgcatcga
     6841 ctccggcaag ccgtgacggc ggttcgcgca gaggaccggc gggcagcagc cagccgttgc
     6901 cgaaaccgcg cgcggcatcc accacgcaga tctcaatgtc gcgcgccagc gcgtagtgct
     6961 gcaacccgtc gtcgctcacc acgacgtcgc aggcgggctg cgccgcgagc agcgagcggg
     7021 ccgcagcgat ccgatccgcg ccggtccaga cctcgcagcc gctgcgacgc gcgagcagga
     7081 ccgcttcatc gccgcacgcg aacggatcgg aatcggaaag cacgcgccgc ggcgcacgcg
     7141 agcggccacc atagccgcgg cacacgatgc ccggcgtgcg gccgcgggca ctcaggaatt
     7201 ccgccagcca gagcgtgagc ggcgtcttgc cggcgccgcc taccgtgaga ttgccgacca
     7261 cgatcaccgg caccggcagc cgggtggtgc cggcgatgcc ggagcggtag gccgcgcggc
     7321 ggatgccgac ggccgcgcca tacgccaggc tcagggggta gagcagggcg gaaaccgggg
     7381 tgacgcgctc ccagtgctgt tcaatccaca ccatgcgcag agtgactaag tgattaagtg
     7441 actaagtgat tgagtaaccc ggcgatcagg ccagccatgg cttaatcacc tagtcaccta
     7501 gtcacttaat cactcacttc ttcccggcct gggtggtgaa ggtgatcttg ccgtagcccg
     7561 cgatgcgcgc cgcctccatg acgttgatca cggactggtg gggggcgttg gcgtcggcgc
     7621 tgatcaccac caccggttcc ttcatgttgc ccgccgcgcg gcgcagctcg gcgctgaagg
     7681 ccgcggcgcc gctgaagccg accggattgc ggttgatcga gtagcgtccc tgggggtcca
     7741 cggcgacgtc gatctggtca ggccgctccg gcgggtgctc ggcgtccgcg gtcggcaggt
     7801 tgatctgcag ttcggcgtag cgcgagtagg tggtggtaac catgaggaag atcaggatca
     7861 ccagcagcac gtcgatcatc gggatgaagt tgatctccgg ctcttcgcgg aaggcgcgcg
     7921 ggcggaacct catacctggc gctctccgtg gatgatttcc accagcttga tggcctgcat
     7981 ctccatgtcc accagcattt cgtccacttt ggcgcggaag tagcggtaga agatcatggc
     8041 gggcaccgcg accaccagcc cgtaggcggt gttatagagc gcgatcgaaa tgccgtgcgc
     8101 gagcaccagc gggttggtgc ccgtcggggt ctgcgcgccg aagatctcga tcatgccgac
     8161 gaccgtgccg aacaggccga gcagcggggc gatgacggcg atggtgccca ggctggtgag
     8221 gaagcgctcg agctcgatcg cgaccgtgcg cccggattcc tcgatcgcct ccttgatgat
     8281 cgggagcggg ctcttggtat tcttgagccc ggcggcgaac acctggccga gcggggagtt
     8341 gttggcgagg cgggagagca tctgcggcgt cacgccgttc tggcggtagt cctgcaccac
     8401 ctgcgccagc aggtttcgcg gcatgacgac ggcttcgcgc agcgaccacc agcgctcccc
     8461 gatgatggcc agcgcgatga cggaggcgat gatgagaaac cagatcggcc acccggcggc
     8521 ttcgatgatg gcaaacacgc gtatgactcc ttcaaaatgg ccgtaactgt atccggcaga
     8581 agcattttca gcaaccggaa atcgctaggc tgatgccggt ggcctgctcc gcgcacgtca
     8641 cccgaaaaat gactttccgt ttttccacaa cggttgtgga taaccatgtg gatactccgt
     8701 gtagcatttg cgtaagtgag gtttccgtat ggaattttgc tgcattgact aaaatttgat
     8761 cgcacctgat ttggaacaca aatcaacggg ttgaccggaa acccgcgccc tgcctgggtt
     8821 tgcgagcagc cattgtgaca aatgcctcac ggctgtggac gaaagaacgc ggtcgcagcg
     8881 accgcgttct ttacgcaact cccgaattca tcaggaaaat ccacgctcga cgagccaata
     8941 tcgggaccca cgacccgggg tcaagcgaga aaaaggccgg aggggctcat ataaggagcg
     9001 ggccgtcacc agcaaccagt ccgaatcact gccttagaat gcggcatgcc cctggcttat
     9061 ccggaactgc cgccggttat ctcggtcgcc gaattgaacc gcatcgcgcg cgacctgctc
     9121 gagcgcaacc tgccgctcct gtgggtggcc ggcgaggtct ccaacttcaa gcgctacgac
     9181 tccggccact gctacttcac gttgaaggac gcacaggccc aggtggactg cgtgatgtac
     9241 cgccaccgcg cccaactgct cggctgggcg cctcaggacg gcatgaaggt ggaggtgcgg
     9301 gcgctcccca cgctgtacga ggcgcgcggc cggttccagc tcaacgtcga ggtggtgcgc
     9361 cgcgccggcc tcggcgcgct ctacgaggcg ttcgagcggc tgaaggccag gctggagaag
     9421 gagggcctgt tcgacctggc gcgcaagcgg cccctgccgc gcttcccgcg cgcggtgggc
     9481 gtggtgacct cgccgcaggc ggc
//
LOCUS       DBNGWZ010000488         5143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1952072, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000488 DBNGWZ010000000
VERSION     DBNGWZ010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1952072"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..417)
                     /locus_tag="AC16CX_15110"
     CDS             complement(193..417)
                     /locus_tag="AC16CX_15110"
                     /inference="COORDINATES: protein motif:HMM:NF018369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HIR3013871.1"
                     /translation="MKTLAVVAMLMLATGPALAQKKDCNELKSEIEAKIKANGVKAFT
                     LEVVPSADVKDGKVVGSCDSGTKKIVYKRG"
     gene            761..1555
                     /gene="pcaD"
                     /locus_tag="AC16CX_15115"
     CDS             761..1555
                     /gene="pcaD"
                     /locus_tag="AC16CX_15115"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HIR3013872.1"
                     /translation="MKIKANGIEINYEIKGNGPWLTMSHSLACDLHMWDPQMPVLTKK
                     FKVLRYDTRGHGRSSAPAGEYTLEQLADDVKGLLDALKIKQTHWAGLSMGGMIGQTFA
                     LKYPGVFASMVLADTTSRRPPDAAKMWSERIQNAQTKGMEALVEGTLGRWFTEPYRKA
                     RPDVMKRIGDGIRNTPVAGFVGCCHAISRIDLTDRLKEIKCPALVIVGEQDMGTPVDA
                     ARAIHQNLPGSELKIIPSAAHLSNVEQADEFNKAMIAFLDRASQRS"
     gene            complement(1682..2839)
                     /locus_tag="AC16CX_15120"
     CDS             complement(1682..2839)
                     /locus_tag="AC16CX_15120"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013873.1"
                     /translation="MAIYLIVLTCVLTHAGFGGAKVALPLHALNLGVDPFSVGVIMAL
                     WALCPMLIALYVGRLVDRVGPRLPMLGGTIGVVVALLVPYFFPGVAALTVMALAVGTA
                     FQFFFVPTQGITGALGGPEDRARNYSLLAIGFSIASFLGPLIAGFSIDYLGYRTAYLM
                     LAACPVAAVLVLWFKGDVLPKAAVGEGEGRKKGGSFDLLRNAKLRDAIVASGLISIAW
                     DLYLFYFPIYGHSIGLSASAIGIVISTFAVAVFAIRVVLPRLARVWTEFQILLYAIGF
                     AGAVFLLFPLFRDPYLLAASSFLLGLGCGVGQPMSMSLIYGLSPPGRASEGAGLRVTF
                     NHFTHLVVPLAFGGIGTAFGFAPVFFSCSAVLIGGSGYGIFTVRQSARSKT"
     gene            complement(3077..3520)
                     /locus_tag="AC16CX_15125"
                     /pseudo
     CDS             complement(3077..3520)
                     /locus_tag="AC16CX_15125"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
     gene            complement(3572..4552)
                     /gene="egtD"
                     /locus_tag="AC16CX_15130"
     CDS             complement(3572..4552)
                     /gene="egtD"
                     /locus_tag="AC16CX_15130"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR3013874.1"
                     /translation="MLLDRRAAAFSIYDRLSDTGSFLDDVVAGLSCPHKTLPPKYFYD
                     ERGSELFEAICGLPEYYLTRAETALMREKAGEMARLLGPRCAVIEYGCGSGRKTRILL
                     EALEPVAYVPIDIAREQLRATAAEFARDFPGLRVMAVCADYSRPLALPEVDVLTARRR
                     IVYFPGSTIGNLTHDEAAAFLGNARNLVGPGGGLLIGVDLKKDTARLNAAYNDKQGVT
                     AEFNINLLARINRELGADFDLGAFRHQAFYNEPLGRIEMHLLSVKAQRVTMRGHSFSF
                     RAGETIHTENSYKYSVSEFQMLARGAGFEPVECWMDAERLFAVHYLAVPQ"
BASE COUNT         1007 a         1655 c         1621 g          860 t
ORIGIN      
        1 cgtgcagacg gtcgccgagt tccaggccgc ggtgcgcggc gcgcagggcg gctatgcggt
       61 gtcgctgctg cgcggcgact tcaacgttac gattatcgtc cgctaattgc cgttaaatcc
      121 ggaccgctgg cgtggccggt ttaactccgc gcgctggaac ggacgaatta ttcgccgttc
      181 aacgcgcggg cgctagccgc gcttgtaaac gattttcttc gttccgctgt cgcagctgcc
      241 gacgaccttg ccatccttca cgtcggcgga aggcacaacc tccagcgtaa acgccttcac
      301 cccattcgcc ttgatcttgg cctcgatctc ggatttgagc tcgttgcagt ccttcttctg
      361 ggcgagcgcg gggcctgtgg ccagcatcaa catcgcaaca acggccaggg ttttcatctt
      421 ggcactcctt tcctgaacaa gttttccaca tgtacgcttt gtagccttcg atcttggccg
      481 gtaagcctcc tgaacagtaa agctttaaaa aacaaatgtt tttgggttag cgaggaccgc
      541 tgacaggtgg gttaataggg gcgggaaaag gaatttcaag tctcccgccc catgaagcgg
      601 atgctggcgg gaaacgtacc agagcccatc ctgcccgagg gataagggcg gagggatgac
      661 ccccgctacg cggaaaagat gctttttccc tcatccttca tccttacgcc gcaccgggtg
      721 cggcggctat actggcgccc tgcttcttac gggagacccc atgaaaatca aggccaacgg
      781 catcgaaatc aattacgaaa tcaagggcaa cggaccgtgg ctcacgatga gccactcgct
      841 cgcctgcgac ctccacatgt gggacccgca gatgccggtc ctcacgaaaa agttcaaggt
      901 gctgcgctac gacacgcgcg gccacggccg gtcgagcgcg cccgccggcg agtacacgct
      961 cgaacagctt gccgatgacg tgaaagggct cctcgacgcg ctcaagatca agcagaccca
     1021 ctgggcaggc ctctccatgg gcggcatgat cggccagacc ttcgcgctga agtaccccgg
     1081 cgtgttcgcg agcatggtgc tcgccgatac caccagccgc cgtccgcccg acgccgcgaa
     1141 gatgtggagc gaacgcatcc agaacgcgca gaccaagggg atggaggcac tggtcgaggg
     1201 cacgctcggg cgctggttca ccgagcccta tcggaaggcg cgcccggatg tcatgaagcg
     1261 catcggagac ggcatccgga acaccccggt cgcgggtttc gtcggctgct gccacgcgat
     1321 ctccaggatc gacctcacgg accgcctcaa ggagatcaag tgcccggcgc tcgtcatcgt
     1381 cggcgagcag gacatgggca cgcccgttga cgcggcgcgc gcgattcacc agaacctgcc
     1441 gggatcggag ctgaagatca tcccttccgc cgcccacctc tccaacgtcg agcaggcgga
     1501 cgagttcaac aaggcaatga tcgccttcct ggatcgtgca tcacagcgga gctgattctg
     1561 gaggactgat ctgcaggatc gcttcgttac gaacgcacgg tgatcattga atgacaagga
     1621 ctgaaccccc gtccccctcg cataccggga gataagggtt atctggtaat ccgcacgcag
     1681 ctcaggtttt gctgcgtgcg gattgacgca cggtaaagat gccgtagccg ctgccgccga
     1741 tcagcaccgc ggaacaggaa aaaaacaccg gggcaaagcc gaatgcggtg ccgatgccgc
     1801 cgaacgcgag cggcacgacg aggtgggtga agtggttgaa cgtcacgcgc aagcccgcgc
     1861 cctccgacgc gcgccccggc ggcgacaggc cgtagatcag cgacatggac atcggctgtc
     1921 cgacgccgca ccccagcccc agcaggaacg acgatgccgc cagcaggtaa ggatcgcgga
     1981 acaggggaaa cagcaggaat accgcgccgg cgaatccgat ggcgtagagc aggatctgga
     2041 actcggtcca gacccgggcg agccgcggca gcaccacccg gatcgcgaac accgcgaccg
     2101 cgaaggtgct gatgacgatt ccgatcgccg aggccgacag gccgatcgaa tggccgtaga
     2161 tcgggaaata aaacaggtac aggtcccacg cgatggaaat gagcccgctc gctacgatgg
     2221 catcgcgcag cttcgcattg cgcagcaggt caaacgaccc ccccttcttc cgcccctcgc
     2281 cttccccgac cgcggccttc ggcagcacgt ctcctttgaa ccacagcacg aggacggcgg
     2341 cgaccgggca ggctgccagc atcaggtagg cggtccggta gccgagatag tcgatcgaga
     2401 agccggcgat gagcgggccg agaaacgagg cgatcgaaaa gccgatggcc agcagcgagt
     2461 aattgcgggc gcggtcctcc ggcccgccca gggcgccggt aatgccctgc gtcgggacga
     2521 agaaaaactg gaaagcggtg cccaccgcca gcgccatcac ggtgagcgcc gccacgccgg
     2581 gaaagaagta cggcaccagc agcgcgacca ccactccgat cgtgcccccc agcatcggca
     2641 gccgcggacc gacgcggtcg acgagcctgc cgacgtagag cgcgatcaac atcgggcaca
     2701 gcgcccacag cgccatgatc acgccgacgg agaacgggtc cacccccaga ttgagcgcgt
     2761 gcagcggcag cgccaccttg gcgccgccga aaccggcgtg ggtcagcacg caggtcagca
     2821 cgatcagata aatcgccatc ggggacggtg cggccgccag gcacccgcac cgcaatcaga
     2881 gatgaaagtc gtggattgta ccgcggatgg aatccgaata gaattcgaat tccacattgt
     2941 gagtaacaga acctgggtga gccggctggg ttctctctat gagcgatcga cgatattcac
     3001 acaggcggcc agcccatgcg gcgttgatag tccataattt ggttatgaaa cgtgaccgcc
     3061 aagcggcccc gtttcatcaa tcggccttgg cgccggtcgc cttgatcacc gggccccact
     3121 tcgcccactc ggccttcaaa aaggcgacaa actgctccgg cggcaggaag gtcgcgtccg
     3181 cgccctgctg ggcgaagcgc tccttgacct cggccagggc gaggatcttt ttcaattccg
     3241 cgctgatgcg gttgaccacc ggtctcgata tcccggccgg cccggagatg ccgtaccgga
     3301 ccgtgccgtc cacctgggga taacccaact cggcaaggct cggcagctcc ggatacttgg
     3361 tcgagcggtt cttgccgacc gtcgccagca gcttgagccg ccccgactgg atgtgcggga
     3421 agatggacgg cagggaatcg aacgcgagct gcacgttgcc cgccgcgagg tccacgacgc
     3481 ccggcccgct gcccttgtac ggcacgtgca cgatcggcgc gccggtggcc gaatcgggtc
     3541 agttcatccc ggtcaatggt tcgcttgcga atcattgcgg gaccgccagg taatggacgg
     3601 cgaacaggcg ttcggcgtcc atccagcact cgaccggctc gaagcccgcg ccgcgggcaa
     3661 gcatctggaa ttcgctcacc gaatacttgt acgaattctc ggtgtggatg gtttccccgg
     3721 cgcgaaagct gaagctgtgg ccgcgcatcg tgaccctttg cgccttcacg ctcagcaagt
     3781 gcatttcgat tcggcccagc ggctcgttgt agaacgcctg gtggcggaac gcgcccagat
     3841 cgaaatcggc gccgagctcg cgattgatgc gcgcgagcag gttgatgttg aattcggccg
     3901 tgacgccctg cttgtcgttg taggccgcgt tgagccgagc ggtgtccttc ttgagatcca
     3961 cgccgatcag cagcccgcca cccggcccga ctaggttgcg ggcgttgccg agaaaggcgg
     4021 ctgcctcgtc atgggtcagg ttcccgatgg tcgagcccgg aaaatagacg atccggcgcc
     4081 gcgccgtgag gacgtcgacc tccggcagcg ccagcgggcg cgagtaatcg gcgcagaccg
     4141 ccatcacccg aagcccgggg aaatcgcgcg cgaattcggc cgccgtcgcc ctcaactgct
     4201 cgcgcgcgat gtcgatcggc acgtaggcca cgggctcgag cgcctcgagc aggatgcgcg
     4261 tcttgcgccc gctgccgcag ccgtactcga tcaccgcgca gcgcggcccg aggagacgcg
     4321 ccatctcgcc ggccttctcc cgcatcagcg cggtctcggc gcgcgtcagg tagtactccg
     4381 ggagcccgca gatcgcctcg aacagctccg agccgcgctc gtcgtagaaa tacttgggag
     4441 ggagcgtctt gtgcgggcac gacaggcccg cgaccacgtc gtcgaggaag ctgccggtgt
     4501 cggaaagccg gtcgtaaatt gaaaaggcgg cggcgcgtcg gtccaagagc atgagcctcc
     4561 tcccatgcgc gccggttacc ggcgtcggat tgataagagc ctataacacc atgaagcgca
     4621 cgtgcgttgc tgccagaatt ggtgatgacg cgaagcgagg tgcagccaat atgtcgatat
     4681 tggcaagccg agcgacaaag tgttccgcgc agggcggagc aggtttcaag cgtttcatgc
     4741 ttggatgcga ccccggagcg gaacaggaga gcgctggaaa ttgcgcgtta ctcacacgcg
     4801 ccttcacggt cgcatccccg gaaaccgggg cccctgctcc acgttccggc gcgtcgccgg
     4861 ttctggcagc aacccttcgg gctggcgcga gttttgcccc atggctttgt cgcaagtccc
     4921 ttgctttgtc ggtctaagcg gcgggccttg cttcgcgcct tgagccaaaa tcgtgccagc
     4981 gcatcgcgcg tttcatggtg ttataggctc tggagtccgc gaggccgcgg ccgggcggtc
     5041 agcccgccgg ctgacgtagg aagttactct ccatccactt cttgatgcgg ttggcgtcgc
     5101 cgatgcgcgt gagcctgccc cacgagtcga gcagcacgat gat
//
LOCUS       DBNGWZ010000489         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_569965, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000489 DBNGWZ010000000
VERSION     DBNGWZ010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_569965"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC16CX_15135"
     CDS             complement(<1..428)
                     /locus_tag="AC16CX_15135"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3013875.1"
                     /translation="MQRRRFLVGVGALGVSGLIGAAEQSAPLRGPDVIFVPTPNEIVD
                     TMLRMAAVTRKDTVYDLGCGDGRIVIAAAQKYGARGVGIDIDPERVAEATENVRKAGV
                     ADRVKIIQGDLFEADISGATVVTLYLLTDLNLKLRPKLLR"
     gene            complement(593..1291)
                     /gene="mtgA"
                     /locus_tag="AC16CX_15140"
     CDS             complement(593..1291)
                     /gene="mtgA"
                     /locus_tag="AC16CX_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR3013876.1"
                     /translation="MLRRVLRWTWRVILLALIALVATQFWFLAHIWYWADHDPESTAF
                     MRARLEIIREDEPHARLAYRWAPYNRISGHLKRAVVAAEDDKFMSHAGFDWEAIQKAH
                     ERNVREGEVVAGASTITQQLAKNLFLSGHRTWWRKAQEAVITSMLEIVLTKRRILEIY
                     LNVVEWGDGVYGAEAAARHQYGVSAAELTPEQAARLAVMLPSPRSYPPGRDTLYLQGR
                     TAVILARMQYARVP"
     gene            complement(1475..>2192)
                     /gene="aroE"
                     /locus_tag="AC16CX_15145"
     CDS             complement(1475..>2192)
                     /gene="aroE"
                     /locus_tag="AC16CX_15145"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR3013877.1"
                     /translation="PRDGFREAVLAFRDTGGKGLNVTLPFKHEAWRLAATRSGFALAA
                     GAVNTLFFQGNQIVGYNTDGIGLVRDLTRNLRIAVRGKRVLLMGAGGASYGVMEPLLR
                     EQPETLVVANRTPEKAITLVGHFEKLQQYALGGISARPYDALAGAQFDILINATSAGL
                     ENAMPPVPENVFAPGALAYDMVYARRTPFLDFAQQRGARVSDGLGMLVEQAAESFFIW
                     RGVYPETRSAMAMLRRRMGR"
BASE COUNT          366 a          732 c          723 g          371 t
ORIGIN      
        1 tcccgcagca gcttgggccg gagcttcaag ttgaggtcgg tgaggagata cagcgtgacc
       61 accgtcgcgc cgctgatgtc cgcctcgaac agatcgccct ggatgatttt cacccggtcc
      121 gcgacgcccg ccttcctgac gttttccgtc gcctcggcga cccgttccgg gtcgatgtcg
      181 atgccgacgc cgcgcgcgcc gtatttctgc gcggcggcga tcacgatgcg gccatcgccg
      241 cagccgagat cgtacaccgt gtccttccgg gtaaccgcag ccatcctgag catcgtgtcc
      301 acgatttcgt tcggagtggg cacgaagatg acgtcggggc cgcgcagcgg ggcggattgc
      361 tccgccgccc cgatgagacc ggaaacgccc agggcgccca cgcccacgag aaaccgtctt
      421 ctctgcatga ggaatctcca atcaagtgcc gatgcggcgc gccattctac ccgatgaggc
      481 gtgatgagtg atgggtgata agagtagtgt accgtgaggg cgggaaagcg tcatggcggg
      541 agagcgaaca gccccccact tacgctctca cgacttcacg atctcacggc tcttacggca
      601 ctcgcgcgta ctgcatgcgc gcgaggatca cggccgtgcg gccctggaga taaagggtgt
      661 cgcggcccgg cggatagctg cgcgggctcg gcagcatgac agcaagccgc gccgcctgct
      721 ccggcgtgag ctccgccgcc gacacgccgt attggtggcg tgcggcggcc tcggcgccgt
      781 acacgccgtc tccccactcg acgacgttga gatagatctc gagaatgcgg cgcttggtga
      841 ggacgatctc gagcatcgag gtgatcaccg cttcctgcgc cttgcgccac caggtacggt
      901 gtcccgaaag aaacaggttc ttcgcgagct gctgggtgat ggtcgaggcg cccgcgacga
      961 cttcgccttc cctgacgttt ctttcgtggg ccttctggat cgcctcccag tcgaagcccg
     1021 cgtggctcat gaacttgtcg tcctcggcgg cgaccaccgc ccgcttgaga tgccccgaga
     1081 tgcggttata cggcgcccac cggtaggcga ggcgggcatg aggctcgtcc tcgcggatga
     1141 tctcgagccg cgcccgcatg aaggcggtgc tctctggatc atggtccgcc cagtaccaga
     1201 tgtgcgcgag gaaccagaat tgcgtcgcga cgagcgcgat cagcgccagc aggatgacac
     1261 gccaggtcca gcgcagaacg cgccgaagca tcggcgctta aaggatgaag ggtgagccgg
     1321 gaattcgacg aacgtgcctg cgcacgttcg agccggcgaa cgcccgacgc gatgcagcgc
     1381 ggcgggccgg ggtcgaagac cagggatgaa ggatgacctg aaccccgtgg ttcgaagcct
     1441 tcagtccgct gccttctgcc ttcatccttc cgccttagcg ccccatccgg cgcctgagca
     1501 tcgccatcgc cgagcgcgtt tccgggtaga cgccgcgcca gatgaagaac gactcggccg
     1561 cctgctcgac cagcatgccg aggccgtcgg agacgcgcgc gccccgctgc tgcgcgaagt
     1621 ccaggaacgg cgtgcgcctg gcatagacca tgtcgtaggc aagcgcgccg ggagcaaata
     1681 cgttttccgg gaccggcggc atggcgttct cgagccccgc cgaggtggcg ttgatcagga
     1741 tgtcgaactg cgcgccggcg agcgcgtcgt acgggcgggc cgaaatcccc cctagcgcgt
     1801 actgctgcag tttctcgaag tgtcccacca gggtgatggc cttctccggc gtgcggttcg
     1861 caacgaccag cgtttccggc tgctcgcgca gcagcggttc catcacgccg taactcgccc
     1921 cgcccgcccc catcaacagc acgcgcttgc cgcgcacggc gatgcgcaga ttgcgcgtga
     1981 ggtcgcgcac gagtccgatg ccgtccgtgt tgtatccgac gatctggttg ccctggaaga
     2041 acagcgtgtt gaccgccccc gcggcaagcg cgaagccgct gcgcgtggcg gcgagacgcc
     2101 aggcctcgtg cttgaaggga agcgttacat tgagtccctt gccgcccgtg tcgcggaacg
     2161 ccaggaccgc ttcgcggaag ccgtcgcgcg gc
//
LOCUS       DBNGWZ010000490         2309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3087601, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000490 DBNGWZ010000000
VERSION     DBNGWZ010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3087601"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="AC16CX_15150"
     CDS             complement(<1..767)
                     /locus_tag="AC16CX_15150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013878.1"
                     /translation="MDLGLKGKVAIVTGGSRGVGRAIAASLLREGARVMISARDPKRL
                     EAARAALERETGGEVRATTADLRHDAEARALIEATLKAFGRLDILVNNAATVMPADFM
                     TLTEERWAEVFEQKLNGYVRCLRHAIPAMKGRGWGRIINLSGLAAREGGSQTIPVGVN
                     NVAVLNLTKSLAFALARDNILVNAVVPYIIDTDIQDETMRRFAALTGRPEADIRKERV
                     AELAVGRMGRPEEVADVVTFLASERSSYVTGAAWHVDG"
     gene            complement(776..1750)
                     /locus_tag="AC16CX_15155"
     CDS             complement(776..1750)
                     /locus_tag="AC16CX_15155"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013879.1"
                     /translation="MLTRIVLIALAAIAASFAGGVAAQQYPARPVRIVVPQPAGGGVD
                     IYTRLISQKLSETWGQQIIVDNRPGANGIIALEQTIKAKPDGYTLLAAFTSVLTINPH
                     VYKSLPYDTFRDLAPITQTVSNTMVLVVHPNLPARSVKGLVALGKSRPGELNYGSFGI
                     GNLTHLAAELLRLEAGLKMVHVPYKGETPAITEAISGQVALLFATSVGVASHISTGRL
                     RLLATCGERRATAFPDTPTMVESGLPKVVVTGWGGTLAPAGTPREIIEKVQRDTARHL
                     QSADLRERLGGMGSDAVGSTPEQFAAFLRNETEKWERVSKVAGIYKSQ"
     gene            complement(1887..>2309)
                     /locus_tag="AC16CX_15160"
     CDS             complement(1887..>2309)
                     /locus_tag="AC16CX_15160"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013880.1"
                     /translation="FEALLSIHYAVAAILHDRALTLAQFEPARYNDPKLARFAAEQVE
                     VKVDPALSGVQAVVEAETADGKTLSVRCDHPRGSPENPLTRAQIEEKFRTYAEGRLSA
                     AHVEEVVGAVARLEDLKSTRRLMDILRAGDEKRVRRSA"
BASE COUNT          337 a          800 c          738 g          434 t
ORIGIN      
        1 ccatccacgt gccacgccgc accggtcacg tagctcgacc gctccgaggc gagaaaggtc
       61 accacgtcgg caacttcttc cggccgcccc atccgcccca ccgccagctc ggccacccgc
      121 tctttccgga tgtccgcttc cggacggccg gtcagggcgg caaaacgtcg catggtctcg
      181 tcctggatgt cggtatcgat gatgtagggc acgacagcgt tcacgagtat gttgtcgcgg
      241 gccagcgcaa acgccagaga cttggtcagg ttgagcaccg cgacgttgtt cacgccgacg
      301 ggtatcgtct ggcttcctcc ctcgcgcgcc gccaggccgg agaggttgat gatccgcccc
      361 cagccgcggc ctttcatcgc gggtatcgca tggcggagac agcgcacgta gccgttcaac
      421 ttctgttcga agacctccgc ccagcgttcc tccgtgagcg tcatgaagtc ggccggcatc
      481 acggtggcag cgttgttcac caggatgtcg aggcggccga atgccttcag cgttgcctcg
      541 atcagggccc tggcttcggc gtcgtggcgg agatccgccg tggtcgcgcg gacctcgccg
      601 cccgtctccc gctcgagcgc cgcccgcgcg gcctccagcc gtttcgggtc gcgcgcggag
      661 atcatgacgc gcgcgccttc ccgcagcagt gacgccgcga tggcgcgtcc cactccgcgg
      721 cttccccccg tgacgatcgc cacctttcct ttgagtccca ggtccatatc gctcgctact
      781 ggctcttgta gatgccggcc accttggaga cccgctccca cttttccgtc tcgttcctga
      841 ggaaagcggc gaactgctcg ggcgtggagc cgaccgcgtc ggaacccatg ccgccgaggc
      901 gctcgcggag atccgcgctt tgcagatgcc gcgcggtgtc gcgctgcacc ttctcgatga
      961 tctcgcgcgg cgtgcccgcc ggggcgagcg tcccgcccca gccggtcacc accaccttcg
     1021 gcagcccgga ttcgaccatg gtcggcgtgt cggggaatgc tgtggcgcgc ctttcgccgc
     1081 aggtcgcaag caggcgcagc cgccccgtgc tgatgtggga tgctacgccg accgatgtcg
     1141 cgaacaacag cgccacctgt ccgctgatcg cctcggtgat cgcgggcgtc tcgcccttgt
     1201 acggcacgtg caccatcttc aggcccgcct cgaggcgcaa cagctcggcg gcgaggtggg
     1261 tgagattacc gatgccgaac gatccgtagt tcagttcacc ggggcgcgat tttccgagcg
     1321 cgaccagccc cttcaccgag cgcgccggca ggttggggtg gaccaccagc accatggtgt
     1381 tgctgaccgt ctgcgtaatc ggtgccagat cgcggaacgt gtcatagggc agcgacttgt
     1441 agacgtgggg attgatggtg agcaccgagg tgaatgcggc gaggagggtg tagccgtcgg
     1501 gcttggcctt gatggtctgc tcgagggcga tgatcccgtt cgcgccgggg cggttgtcca
     1561 cgatgatctg ctgcccccag gtttcgctca acttctgcga aatgaggcgc gtatagatgt
     1621 ccacgccgcc gccggccggc tgtggaacga cgatccgcac cggccgggcg gggtactgct
     1681 gtgcagctac cccgcccgcg aaggatgccg caattgctgc aagcgcaata aggacgatcc
     1741 gtgtcaacat ttcttcatcc tccagttgtt cgtgagatcg ggagatcggg agagaggtac
     1801 ttcgctcttt cgccctcacg cccttccgct ctcacggatt ttgtcgctct cccgccctcc
     1861 cgcactcacg ccctcccgtg ttttggctac gcggagcggc gcacccgctt ctcgtcgccc
     1921 gctctcagga tatccatcag cctgcgcgtg gatttgaggt cttccagcct cgcgacggcg
     1981 ccgacgactt cctcgacgtg cgccgcggac agcctgccct ccgcgtaggt gcggaacttt
     2041 tcctcgatct gcgcgcgcgt aagcgggttc tcgggcgagc cccgcgggtg gtcgcagcgc
     2101 acggacaggg tcttgccgtc ggcggtttcc gcctccacga cggcctgaac gccactcagg
     2161 gctggatcga ccttgacttc cacctgctcg gcggcgaagc gcgcgagctt cggatcgttg
     2221 tagcgcgccg gctcgaactg ggcgagcgtc agcgcccggt cgtgcagaat cgccgccacg
     2281 gcatagtgga tcgaaagcag cgcctcgaa
//
LOCUS       DBNGWZ010000491         2207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2707490, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000491 DBNGWZ010000000
VERSION     DBNGWZ010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2707490"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="AC16CX_15165"
     CDS             <1..374
                     /locus_tag="AC16CX_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3013881.1"
                     /translation="SSGLYVERLLGELGLLERIQDRTTRCGNGPEMMEHLINGKGREI
                     GFGAIIEILLFRDKGLKLVGPLPPEIQHYTDYLAVPMTAAPNPAGAKTFTRYLATPEA
                     RTLFAAHGIEQSFPKRKLPRS"
     gene            377..2194
                     /locus_tag="AC16CX_15170"
     CDS             377..2194
                     /locus_tag="AC16CX_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3013882.1"
                     /translation="MGQITVARALAKLLEKMGTEVVFGVNGHGNWALLDAVVHETRIR
                     GVPARAEDHAIQMADGFWRMRRGGPLSVVTTSVGPGNMNIVPAVAAAFYESIGLLVIA
                     SAGATHWFDRGGIEEAYRYGPEDWVAVLKPITKKSFMMTRPDNALDMFLRAYHTAISG
                     RPGPVVVQIPYDIQNTLIADKLPDPRPFMQWHPPAPDPDGIRKAAALLKAAKRPFLAV
                     GSGIHNARAWDELLSFAEAAGIPVATTATGKGAFPEGHRLSVGCIGRAGTGHGNAAAR
                     RADVVVGVGTHFSDIDTGGWTLFDIPARTRLIHLDIDTTELGRAYPTAVALTCDARLG
                     LKALTEAARKAGIRERGDWLKEIAQERKSWEESVADQRHSAISPLHYARICHDTAEVV
                     AAKNPQMPILFDTGHLLSFAPPFLKASSRHIAHNGYFHRMGWSASAVIGASIAQGNRP
                     ALALIGDGSFMMGGTAVATAVEQNLPIVWVVFNNRSLQIERELMFRLYGREAFCDYRR
                     KGQKGLWNPDFCKWAESMGAAAIHVTQAAKYAPALRRALDNRAPTVISVEVSLDIKGY
                     RSIWYPYPNDFHTAWRPGPLPASSAVPAGKVHGRRAGRH"
BASE COUNT          388 a          720 c          748 g          351 t
ORIGIN      
        1 cttcgagcgg gctttacgtc gagaggctgc tcggggaact ggggctgctc gagcgaatcc
       61 aggacaggac gacccgctgc ggtaacgggc cggagatgat ggaacatctg atcaatggga
      121 agggccggga gatcggcttc ggcgccatca tcgaaatcct gctcttccgc gacaagggcc
      181 tgaagctcgt tggcccgctg ccgccggaaa tacagcacta taccgattac ctggcggtgc
      241 cgatgaccgc ggcgcccaac cccgctggcg cgaaaacgtt cactcgctat ctggcgacac
      301 cggaggccag aacccttttt gccgctcacg gtatcgaaca gtcgtttcca aagagaaaat
      361 tgccaaggag ttgatcatgg gacaaatcac ggtagcgcga gctctggcca agttgctgga
      421 gaagatgggg accgaagtcg tgttcggcgt gaacggccac ggcaactggg cgctgctcga
      481 cgcggtggtg cacgagaccc ggatccgcgg cgtgccggcg cgtgccgagg accatgcaat
      541 ccagatggcg gacggtttct ggcgcatgcg gcgcggcgga ccgttgtccg tcgtgacgac
      601 cagcgtcggt cccggcaaca tgaacatcgt tcccgccgtg gccgctgcgt tctatgaatc
      661 gatcggcctg ctggtgatcg caagcgcggg cgcgacccac tggttcgacc ggggcgggat
      721 cgaggaggcc tatcgctacg gccccgagga ctgggtggcg gtgctgaagc cgatcaccaa
      781 gaaatccttc atgatgacgc gcccggacaa cgcgctcgac atgttcctgc gcgcctacca
      841 cactgccatt tccgggcgtc ccggcccggt ggtcgtccag atcccgtacg acatccagaa
      901 cacgctgatt gcggacaagc tgcccgatcc ccggccgttc atgcagtggc atccgccggc
      961 gccggatccg gacggcatcc ggaaggcggc cgcgctgctg aaggcggcga agcgcccgtt
     1021 cctcgcggtg ggcagcggca tccataatgc gagggcctgg gacgagttgc tcagcttcgc
     1081 cgaggcggcc ggaattccgg tcgccaccac cgccaccggc aaaggcgcgt tcccggaagg
     1141 gcaccggctc tccgtcggct gcatcggccg cgccggcacc ggccacggca acgcggccgc
     1201 gcgccgcgcc gatgtcgtcg tcggcgtggg cacccatttc agcgacatcg acactggggg
     1261 ctggacactg ttcgacatcc cggcccgcac gcgcctgatc cacctcgaca tcgacaccac
     1321 ggagctcggc cgggcctacc cgacggcggt cgcgctgacc tgtgatgcgc gtctcggttt
     1381 gaaggcgctg accgaggcgg cgcgcaaggc gggcattcgc gagcgcggcg actggttgaa
     1441 ggagatcgcg caggagcgga aatcgtggga agagtcggtg gcggaccagc gtcactccgc
     1501 catctcgcct ctgcactacg cccgcatctg ccacgacacc gcggaggtcg tggcggcgaa
     1561 gaacccgcag atgccgatcc tgttcgatac ggggcatctg ttgagcttcg cgccgccgtt
     1621 cctgaaggcg tcgtcgcgcc acatcgccca caacggctac ttccatcgca tgggctggag
     1681 cgcctcggcc gtcatcggcg cgagcatcgc gcagggcaat cgcccggcgc tggcgctgat
     1741 cggcgacggc tcgtttatga tgggcggcac cgcggtcgcg acggcggtcg agcagaacct
     1801 gcctatcgtc tgggtggtat tcaacaaccg gtcgctgcag atcgagcgcg agctgatgtt
     1861 ccggctctac ggccgcgagg cattctgcga ttaccggaga aaaggccaga aggggctgtg
     1921 gaatccggac ttctgcaagt gggccgaatc gatgggcgct gccgccatcc acgtcacgca
     1981 ggccgcgaag tacgcgcccg cgctgcgccg cgcgctcgac aaccgcgcgc ccacggtgat
     2041 cagcgtcgag gtgtcactcg acatcaaggg ctaccgttcc atctggtatc cctacccgaa
     2101 cgatttccac acagcctgga ggccgggacc gctgccggcg tcgagtgccg tgccggccgg
     2161 taaggtacac ggcagaaggg cagggcggca ttgagcggtg agctgcg
//
LOCUS       DBNGWZ010000492         5150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1325260, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000492 DBNGWZ010000000
VERSION     DBNGWZ010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1325260"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16CX_15175"
     CDS             complement(<1..290)
                     /locus_tag="AC16CX_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013883.1"
                     /translation="MNKIILGACCLMLAASTPALAGDPAAGQKKSQACVACHGPDGNS
                     PAPDFPKIAGQHYDYLLKALKDYKSGARKSPVMAPMIANLSPRDMEDLAAYFS"
     gene            complement(302..652)
                     /locus_tag="AC16CX_15180"
     CDS             complement(302..652)
                     /locus_tag="AC16CX_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013884.1"
                     /translation="MKIPDLISIAAACCLFTAALPLHAAEAGDPAAGKNKNSMCIGCH
                     GIPGYHTVFPEVYHVPKLGGQHAAYIVKALQEYKSGNRTHPTMRAIAAGLSDKDMADL
                     AAYYASDATKAAAK"
     gene            782..2581
                     /locus_tag="AC16CX_15185"
     CDS             782..2581
                     /locus_tag="AC16CX_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3013885.1"
                     /translation="MTPRQSAAAASRFGRLFPFLRWWPRVNRETLRADAIAGFVGAII
                     VLPQGIAFATLAGLPPQYGLYAAMVPAIVAALFGSSWHLVSGPTNVISIFLFASLVPL
                     AEPGSNDYISLALTLTLLVGVMQFAMGLARMGALINFISHTVVVSFTAGASCLIFAAQ
                     LKHFFGVEIPSGASFARTWYDFALGLDHANPYVTAVGAVTLLTGTLFRRNAPRFPYMI
                     VAMIVGGIFAALLNAAFGEARTGIAMVGALPGSLPGLSAPDLSAETVRKTLPVALAVT
                     ILALTEAVSIARAIAVKSGQRIDGNQEFVGQGLSNIVGSFFSAYASSGSFNRSGVNFE
                     AGARTPLAAAFSALMLAPIVFAVASLVAYLPLAVMAAILFLVAYGLIDLRYIATVLRT
                     SRQETTVMLLTFLAALFANLEFAIYAGVLLSLMLYLTRTSRPLLLDVKPDPAPDSYHF
                     TADSGLPDCPQLKMLRVNGSLFFGAVDHVQRNLEQIDEIEPRQKHVLIVASGINFVDL
                     AGAEMLARESRRRRSIGGGLYFYRVKDAVRELLARGGYLREIGEENLFPVKSRPVAAI
                     YRKLDSEICRNCKARIFRECHVSLPNGEPVKAA"
     gene            complement(2600..3676)
                     /locus_tag="AC16CX_15190"
     CDS             complement(2600..3676)
                     /locus_tag="AC16CX_15190"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3013886.1"
                     /translation="MIPVEQAAIQFIDLQYGNVLALLAIGFVGGLVSGFIGSGGAFVL
                     TPAMMSMGVPGVMAVASNMCHKFPKALVGAHKRHKFGHVDLKLGLVMAASAAVGVWLG
                     ADIQHQIKENFGEPGSNLYVSLAFVIVLTIVGGFVLHDAIRIYRSGDASGEDKASRLA
                     RWVQSVEIPGTMMHFKTADVRLSALFTLPLGFATGLLASIIAVGGFIGVPGMIYVLGA
                     PGLIASATELVIAFVMGLGGTIKYALHGLVDIRLALILLAGSLFGIQLGAIGTTFVKP
                     FMIKVVMAAIMLIVAVSRGLVVPVYLTQLGGMDLSPQTMGWLHTVSFAFLVLALCTGG
                     IIIIASMLRGHRIRTERLEEAASE"
     gene            complement(3673..3903)
                     /locus_tag="AC16CX_15195"
     CDS             complement(3673..3903)
                     /locus_tag="AC16CX_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013887.1"
                     /translation="MQNSKPYPQLVASGIVSGSLYLLLYLYEREILAAFTRTDGWYPA
                     LPVIAALVFSFAHGAFTAYFWEVLGITGRRGR"
     gene            3983..4534
                     /locus_tag="AC16CX_15200"
     CDS             3983..4534
                     /locus_tag="AC16CX_15200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3013888.1"
                     /translation="MANRLSKIYTRTGDGGTTGLADGSRVAKDSPLIEAIGAVDELNS
                     VIGLLLSEEPPEEVRACLTDVQHDLFDLGGELSKPGHTILTEAQVTRLERTLDRFNKE
                     LPPLKDFILPGGSRAAALAHVARAVCRRAERRLAALSREEKPAPVMLAYLNRLSDLLF
                     VLARTLNRHAGRPDVLWQQGKNR"
     gene            4750..>5150
                     /locus_tag="AC16CX_15205"
     CDS             4750..>5150
                     /locus_tag="AC16CX_15205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3013889.1"
                     /translation="MRRQDWAEEVLQECYVSIWNHAGDYAAAKSAPLTWMTSIVRNRC
                     LDWLRRPQTEATGEEYDVAVEAWRDDAPGPMESLMAASDAAALARCMQQLESKQRQSI
                     MLAFFHGLSHSELASHMKQPLGTVKTWVRRGL"
BASE COUNT          892 a         1731 c         1622 g          905 t
ORIGIN      
        1 gaaaagtagg cggccagatc ctccatgtcg cgcgggctga ggttcgcaat catgggcgcc
       61 atgaccgggc tcttgcgcgc gcccgacttg tagtccttca gcgccttcag caggtaatcg
      121 taatgctggc ccgcgatctt ggggaagtcc ggcgccgggc tgttgccgtc ggggccatgg
      181 cacgccacgc acgcctgtga cttcttctgc cccgccgccg gatcaccggc aagagcgggc
      241 gtgctcgccg cgagcatcag gcagcacgcg cccaggataa tcttgttcat ttctcctccc
      301 ctcacttggc cgccgccttg gttgcatcag aagcgtaata ggcagccagg tcggccatgt
      361 ccttgtccga gaggcccgcc gcgatcgcgc gcatcgtcgg gtgagtgcgg ttgccggact
      421 tgtattcctg cagcgccttg acgatgtagg ccgcatgctg gccgccgagc ttgggcacgt
      481 gatagacctc ggggaaaacg gtatggtatc cgggaatgcc gtgacagccg atgcacatcg
      541 agttcttgtt cttgcccgcg gccgggtctc cggcttcggc ggcgtgcagc ggcagcgccg
      601 cggtgaacag acaacaggcg gccgcgatgg aaatcagatc gggaattttc atggtcgcgc
      661 tttcttgtgc ttgagccgct gcgcgcgatt atacccaaaa aatcgcgtgg cggtaccgcg
      721 cgttgccagc gccgcgcgcg ctgccggata atgcgtcatt caaccgaact ctcctcgcaa
      781 cgtgacgcca cgccaatccg cggcggccgc aagccggttc ggccggctgt ttccgttcct
      841 gcgctggtgg ccccgtgtaa accgcgagac gctgcgcgcc gatgccatcg ccggctttgt
      901 cggcgcgatc atcgtgctcc cgcagggtat cgcgttcgcc actctggcag gcctgccgcc
      961 gcagtacggc ctctacgccg ccatggtgcc ggcgatcgtg gcggcgctgt tcggatcctc
     1021 gtggcacctt gtttccgggc cgacgaacgt gatctcgatt ttcctgttcg cgagcctcgt
     1081 ccctctcgcg gaaccaggca gcaacgacta catcagcctt gcgttgacac tcaccctcct
     1141 ggttggggtc atgcaattcg ccatgggact ggctcgcatg ggcgctctga tcaatttcat
     1201 ttcgcacacg gtcgtggtga gcttcaccgc cggcgcatcc tgtctgatct tcgccgctca
     1261 gctcaagcat ttcttcggcg tcgaaatccc gtccggcgcg tccttcgccc ggacttggta
     1321 cgatttcgcc ctcgggctcg atcacgccaa cccctacgtg acggcggtgg gggcggtcac
     1381 tctgctgacg gggactctct tccgcaggaa cgcgccacgc tttccctaca tgatcgtcgc
     1441 aatgatcgtg ggcggcatct tcgcggcgct gctgaacgcc gccttcggcg aggccaggac
     1501 cggcatcgcg atggtggggg cgctgcccgg ctcgcttccc gggctttccg cgccggacct
     1561 ctctgctgag acggttcgca agaccctgcc ggtcgcgctc gcggtgacga tactcgcgct
     1621 cactgaagcg gtgtcgatcg cgcgcgccat cgcggtgaag tccgggcagc gcatcgacgg
     1681 caaccaggag ttcgtcggac aggggctgtc caacatcgtc ggcagctttt tctccgctta
     1741 cgcctcgagc ggctctttca accgcagcgg tgtgaacttc gaggcgggcg cgcgcacgcc
     1801 gctcgcggcc gcgttttcag cgctcatgct cgcgccgata gtgtttgccg tggcctccct
     1861 cgttgcgtat ctcccgctcg cggtcatggc ggcgatactg ttcctcgtgg cgtacggcct
     1921 catcgattta cgctacatcg cgaccgtgct caggaccagc cgtcaggaga cgacggtgat
     1981 gctgctcacg tttctcgcgg cgctcttcgc caacctcgag tttgcgatct acgccggcgt
     2041 gctgttatcg ctgatgctgt acctcacgcg cacctcgcgt ccgctgctgc tcgacgtcaa
     2101 gcccgatccc gcgccggaca gctaccactt caccgccgac agcggcctgc cggattgccc
     2161 gcagctgaag atgctgcggg tgaacggctc gctctttttc ggcgccgtcg atcacgtgca
     2221 gcgcaacctg gaacagatcg acgagatcga gccgcggcag aaacacgtgc tgatcgtggc
     2281 gagcggtatc aacttcgtgg atctggcggg cgcggaaatg ctggcacgcg aatcccggcg
     2341 gcgccggagc atcgggggcg ggctgtactt ttatcgcgtg aaggacgcgg ttcgggagct
     2401 gctcgcgcgc ggcggctacc tgcgcgagat cggcgaggaa aacctgtttc ccgtgaagag
     2461 ccgcccggtt gcggcaattt accgcaagct cgactccgag atctgccgca actgcaaggc
     2521 gcgcatcttc cgcgagtgcc acgtgagcct gcccaacggg gagccggtga aggcggcgta
     2581 agcgacgccc cgccgccgcc tactcgctgg cggcttcctc gaggcgctcc gtccgtatgc
     2641 gatgcccgcg cagcatggag gcgatgatga tgatgccacc cgtgcacagc gcgagcacca
     2701 ggaacgcgaa actcaccgta tgcagccagc ccatggtttg cggcgacaga tccatcccgc
     2761 cgagctgcgt gaggtaaacc ggcaccacca agccgcgact caccgcgacg atcagcatga
     2821 tcgcggccat cacgaccttg atcatgaacg gctttacgaa ggtggtgccg atcgcgccga
     2881 gctggatgcc gaaaagcgag cccgcgagca ggatcaacgc gagccggatg tccaccagcc
     2941 catgcagcgc gtacttgatg gtaccgccaa gccccatcac gaacgcgatc accagctcgg
     3001 ttgcgctcgc gatgaggccc ggcgcgccca gcacgtagat catgcccggc acgccgataa
     3061 agccgccgac cgcgatgatc gaagcgagta atcccgtcgc gaatcccagc ggcagggtga
     3121 acagcgccga cagccggacg tccgccgtct tgaagtgcat catggtgccg gggatctcga
     3181 ctgactgcac ccagcgcgcg agccgtgagg ccttgtcctc cccgctggca tcgcccgagc
     3241 gataaatcct gatcgcgtcg tgcaggacga agccgccgac gatggtgagc acgatgacga
     3301 aggcgaggct cacgtacagg ttcgaacccg gctcgccgaa gttctccttg atctggtgct
     3361 ggatgtccgc gcccaaccat accccgacag ccgccgaggc cgccatcacc agcccgagct
     3421 tgaggtccac gtgcccgaac ttgtggcgct tgtgggcacc gaccagggct ttcggaaact
     3481 tgtggcacat gttgctcgcc accgccatga cgccgggcac gcccatgctc atcatggcgg
     3541 gcgtcaacac gaaggcgccg ccggagccga tgaagccgct caccagcccg ccgacgaaac
     3601 cgatcgcgag cagggcaagc acattgccat actgcaggtc tatgaactgg atcgcggcct
     3661 gttccaccgg gatcaccgcc cgcgcctgcc tgtgatgcca agcacttccc agaaatatgc
     3721 cgtgaatgcg ccatgcgcga aggaaaacac taacgccgcg atgaccggca gcgcgggata
     3781 ccagccgtcg gtgcgcgtga acgccgccag gatttcccgc tcgtacaggt agagcagcag
     3841 atagagcgag ccggagacga ttcccgatgc gaccagttgc ggatagggtt tcgaattttg
     3901 cacgccgctc accctcttcg cgcatcatag caaatgcgcg gcggttgcac ctttatgccg
     3961 ctataattcc ggcttatcgc ccatggccaa tcgcctctcg aaaatctaca cccgcaccgg
     4021 cgacggcggc actactgggc tcgccgacgg ctcgcgcgtg gcgaaggatt cgccgctgat
     4081 cgaggcgatc ggcgcggtgg acgagctgaa cagcgtgatc ggcctgctgc tctccgagga
     4141 accgcctgaa gaagtgcgcg cgtgcctcac cgacgtgcag cacgacctgt tcgatctcgg
     4201 aggcgagctg agcaagcccg ggcataccat cctgaccgaa gctcaggtga cgcggctcga
     4261 gcgcacgctc gaccggttca acaaggagct gccgccgctc aaggacttca tcctgcccgg
     4321 cggctcgcgc gcggcagcgc tcgcccacgt cgcgcgcgcc gtctgccgcc gcgccgagcg
     4381 gcggcttgcc gcgctttcgc gcgaggaaaa gccggcgccg gtcatgctcg cctatctcaa
     4441 ccggctgtcc gacctgctct tcgtgcttgc acgcacgctc aaccggcacg ccggacggcc
     4501 cgacgtgctc tggcagcagg gcaagaaccg atgaccgaaa aatccgggag ttcggaagag
     4561 cgggagatcg ggagatcggg ttccgtctcg ccctcacgca ctaacgccct cccgccctca
     4621 cgcactccgg acgccctcgc tctgaggctt gccgagctgc tggtccagtg cacgctcaaa
     4681 aaccagcgtg cattcgcgga cttatatcaa ctcacctcgg cgaaactata tggggtcgcg
     4741 ctccgtatat tgagaagaca ggactgggcc gaagaggtgc tgcaggagtg ctacgtcagc
     4801 atatggaacc acgccgggga ctacgcggcg gcgaagagcg cgccgctcac ctggatgacg
     4861 agcatcgtgc gcaaccgctg cctcgactgg ctgcggcggc cgcaaacgga agcaaccggc
     4921 gaggaatacg acgtcgcagt ggaggcatgg cgggacgacg cgcccgggcc gatggagagc
     4981 ctgatggccg cctcggatgc ggccgcgctt gcacgctgca tgcagcagct ggagagcaag
     5041 cagcgccaga gcatcatgct cgcgtttttc cacggcctga gccactcgga actcgcgagc
     5101 cacatgaagc agcccctcgg caccgtgaag acgtgggtgc ggcgagggct
//
LOCUS       DBNGWZ010000493         2713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1952348, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000493 DBNGWZ010000000
VERSION     DBNGWZ010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1952348"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="miaA"
                     /locus_tag="AC16CX_15210"
     CDS             <1..732
                     /gene="miaA"
                     /locus_tag="AC16CX_15210"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR3013890.1"
                     /translation="SAARFRDDALALMREITERDRIPLLVGGTMLYFRALTEGLNELP
                     EADPMIRLMIDTMAEEQGWPAVHAKLRRVDPETASRLDPGDAQRIQRALEVYYLTNQP
                     MSVLLKKPKYVYFPYSPIRISLVPSDREILHTRIERRFDAMLEAGLVAELRKLREEYA
                     LEADMPSMRCVGYRQAWDHLEGRIDLAALREQGVAATRQLAKRQLTWLRAMDDVTEFD
                     CLTEDLADLVLEYVRRELEAQLAGG"
     gene            complement(858..1598)
                     /locus_tag="AC16CX_15215"
     CDS             complement(858..1598)
                     /locus_tag="AC16CX_15215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3013891.1"
                     /translation="MEIVSQGGHFFTENQNSDRMYLLVDGEVSLIRGKKVLDVVRAGE
                     VFGELAAISQLSRSATAAAKTACRALSLDARQFQSAIQNTPEFALMLMNIIINRLRLT
                     VALAVNKAPDDSAGLRERRVFDEKLLAELMGKLHHRSPVHHPLNKVIMKEGEGGVFMY
                     VVLEGRVAISVKSKIVERVGPGGVFGEMALVDQSPRAATATAVTDCTLLSINRGDFLT
                     LVKSNPAFAVSLLKAAADRLRLMTSHHK"
     gene            complement(1710..>2713)
                     /gene="xseA"
                     /locus_tag="AC16CX_15220"
     CDS             complement(1710..>2713)
                     /gene="xseA"
                     /locus_tag="AC16CX_15220"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3013892.1"
                     /translation="LYEAFERLKARLEKEGLFDLARKRPLPRFPRAVGVVTSPQAAAL
                     RDVLTTLRRRMPRLPVIIYPTPVQGEGAAERVAAAISLAGSRGECDVLIVCRGGGGIE
                     DLWAFNEEAVARAILACAVPVVTGIGHETDFTIADFVADARAPTPTGAAALASPDRDE
                     LAKRLSHAVHRLLRATRRGLEDRMQRLDFLSRRLVHPGERIRSQLGELRHLANRLVGA
                     WERGLEDLGWRTRELGLRLIGCRPDTPGLEREAAELARRLREGARRRLEAVAALLERF
                     DAHLEHLNPRSVLERGYSMTEAAGGRIIRDAAQLAVGEDVRITFSKGWAGARITRKG"
BASE COUNT          444 a          965 c          848 g          456 t
ORIGIN      
        1 tccgcggcgc gcttccgcga cgacgcgctg gcgctgatgc gcgagatcac cgagcgcgac
       61 cgcatcccgc tgctggtggg cggcaccatg ctctacttcc gggcgctgac cgaagggctc
      121 aacgagctgc cggaggccga cccgatgatc cggctgatga tcgacaccat ggccgaggaa
      181 cagggctggc cggccgtgca cgcgaaactg aggcgcgtgg acccggagac cgcgtcccgg
      241 ctcgatcccg gcgatgcgca gcgcatacag cgcgcgctgg aggtctatta cctgacgaac
      301 cagccgatgt ccgtcctgct gaaaaagccg aaatacgtct attttcccta cagcccgatc
      361 cggatttccc tggttcccag cgatcgcgag atcctgcaca cgcgtatcga gcggcgcttc
      421 gacgccatgc tcgaagcggg cctcgtggcg gagctcagga agctgcgcga ggagtacgcc
      481 ctggaagccg acatgccttc gatgcgttgc gtcggctacc gccaggcctg ggaccacctg
      541 gagggccgga tcgatctcgc ggcgctgcgc gagcagggtg tcgccgccac ccgccagctc
      601 gccaagcgcc agctcacctg gctgagggcg atggatgacg tgactgaatt cgactgcctg
      661 accgaggatc tcgccgacct ggtgctcgaa tacgtgcggc gcgagctgga agcccagctc
      721 gctggcggct aacgggcaag tcgaaagtcg aaagtaagaa gggctgcaac cctttcgcct
      781 tgctcgtcaa ctgcctggca gttttccctt ccgccttccg cctttcgact ttcgacttcc
      841 gacttaatga cttcgcatca cttgtgatgt gaagtcatca gccgcagccg gtcggcagcc
      901 gccttgagca acgacacggc aaaagcagga ttgcttttca cgagcgtcag gaagtcgcct
      961 cgattgatcg acaacaaggt gcagtcggtt acggccgtgg cggtcgcggc gcggggggac
     1021 tggtccacca gggccatttc tccaaataca cctcccggac ccaccctctc cacgatcttg
     1081 gacttgacgg agatcgcgac cctgccctcc agcaccacgt acatgaacac acctccctcg
     1141 ccttccttca tgatgacttt gttgaggggg tggtgtaccg gagagcggtg atgcagcttg
     1201 cccatcagtt cggccagaag cttctcgtca aagaccctgc gctcgcgcaa ccccgcgctg
     1261 tcatccgggg ccttgttcac ggccagggcc acggtcagac gcaggcggtt gatgatgatg
     1321 ttcatcagca tgagcgcgaa ttccggcgta ttctggatcg cgctctggaa ctgcctggcg
     1381 tcgagggaca gcgcgcggca ggcggtcttg gccgccgccg tcgcgctgcg ggacagctgg
     1441 ctgatcgctg ccaattcgcc gaacacctcg cccgccctga cgacgtccag cacctttttc
     1501 ccgcggatca ggctcacctc gccatccacc agcaggtaca tcctatcgct gttctgattc
     1561 tcggtgaaga aatgtccgcc ctgcgagacg atctccacct tgcccaggga ctggaaacag
     1621 gccttggcaa tggccggatc gtagatcgtg cttttcgcgg gcctactcgt gaagtctagg
     1681 tcatccattc cgccctcatc cttccgcttt catcctttgc gcgttatccg cgcgcccgcc
     1741 caacccttcg agaacgtgat cctcacgtcc tccccgacgg caagctgcgc ggcgtcgcgg
     1801 atgattctac cgcctgcggc ctcggtcatg ctgtagccgc gctccagcac cgatcgcggg
     1861 ttgagatgct cgaggtgcgc atcgaagcgc tcgagcagcg ccgccacggc ttcgagccgc
     1921 cgccgcgcgc cttcccgcag ccgccgcgcc agctccgcgg cttcccgctc gagtcccggc
     1981 gtgtcgggcc tgcagccgat caaccgcaac ccgagctcgc gcgtccgcca gcccaggtct
     2041 tccagcccgc gctcccaggc cccgacgaga cggttcgcga ggtgccgcag ctcgccgagc
     2101 tggctccgga tacgctcccc agggtgcacc agccggcgcg aaagaaaatc gagccgctgc
     2161 atgcggtcct ccagccccct gcgggtggcg cgcagcaggc ggtggacggc atgcgacagc
     2221 cgcttcgcca gctcgtcccg gtcggggctc gccagcgcgg ccgcgcccgt cggcgtcggc
     2281 gcacgcgcgt ccgcgacgaa atcggcgatg gtgaaatcgg tctcatgacc gatcccggtc
     2341 acgaccggga ccgcgcaggc gaggatggcg cgggccacgg cctcctcgtt gaacgcccac
     2401 agatcctcga tgccgccgcc gccccggcac acgatcagca cgtcgcattc cccccgcgag
     2461 ccggctagcg agatcgccgc agcgacgcgc tctgccgcgc cctcgccctg caccggcgtg
     2521 gggtagatga tgaccggcag ccgcggcatg cgacggcgca gggtggtcag gacgtcgcgc
     2581 agggcggccg cctgcggcga ggtcaccacg cccaccgcgc gcgggaagcg cggcaggggc
     2641 cgcttgcgcg ccaggtcgaa caggccctcc ttctccagcc tggccttcag ccgctcgaac
     2701 gcctcgtaga gcg
//
LOCUS       DBNGWZ010000494        12559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2963251, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000494 DBNGWZ010000000
VERSION     DBNGWZ010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12559
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2963251"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2065)
                     /gene="metG"
                     /locus_tag="AC16CX_15225"
     CDS             complement(<1..2065)
                     /gene="metG"
                     /locus_tag="AC16CX_15225"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3013893.1"
                     /translation="MPRSIFLTSALPYANGAIHLGHLVEYIQTDIWVRFQRMRGHEVH
                     YCCADDTHGTAIMLRAEQDGITPEALIARVLEEHKRDFKGFHVEFDNYYTTHSDETRT
                     LSEEFYRKLDAAGLIHKKPVEQFFDPVKNLFLPDRFIKGECPKCGARDQYGDACENCS
                     STYSPTDLKNPYSVVSGAKPVRKTSDHYFFKLSDPRCRKFLEQWTQEDEHLQPEARNK
                     IREWFDQGLNDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYFGSLKNLAVRNKKIE
                     FDAFTKAGAKTEMIHFIGKDILYFHALFWPAMLEFAGYRAPSHLYVHGFLTVNGEKMS
                     KSRGTFITAESYLKQGLNPEWLRYYYAAKLGPTMGDIDLNLDDFAARVNSDLVGKYIN
                     IASRTLPFLTKYFEGKIGSSLANPTEFDARLARAIAGADRSADEGEHKDFLAYLRAES
                     ATADALLATVQKSSPRIKELYEDREFGKAVREIMMLADYINVYIDHKKPWEIAKNPNQ
                     HSELHKVCSCTIDAFRLLTIYLKPVLPNLASHVEQMLGISELKWDDAQKHLEQGHKTK
                     PYVHLMTRIDPKQITTLVEASKESLQPAPQPHSQQRHAEHQAHAAKKAGGPVSTQTPP
                     SPLPPLPEGEGKSTGAGAQITIDDFNKVDLRVAKIVNAEPVEGADKLLRLTLDLGDGT
                     RTVLAG"
     gene            2183..3268
                     /gene="apbC"
                     /locus_tag="AC16CX_15230"
     CDS             2183..3268
                     /gene="apbC"
                     /locus_tag="AC16CX_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR3013894.1"
                     /translation="MVTEQQVHDVLKQLTDPNTRKDFVSTKSARNIKVDGDKVSLEIL
                     LGYPAKSQLAPIREAVTQALRAIPNVAGVNVNVQMKIVSHAVQRGVKLVPGIKNIIAV
                     ASGKGGVGKSTVAVNLALALAAEGASVAVLDADIYGPSQPTMLGIRGRPESRDGKTLE
                     PMEGHGVQAMSIGFLIDLETPMVWRGPMVTQALEQLLNETKWREVDYLVVDLPPGTGD
                     IQLTLAQRVPVTGAIIVTTPQDIALIDARKGLKMFEKVGIPILGVVENMSLHICSKCG
                     HEERIFGEGGGERMSKDYNVEMLGALPLDIRIREQADSGKPTVVSDPDGRVAQIYKQI
                     ARRVAVKIAEKAQDHTAAFPKIVIQNT"
     gene            3363..3935
                     /gene="dcd"
                     /locus_tag="AC16CX_15235"
     CDS             3363..3935
                     /gene="dcd"
                     /locus_tag="AC16CX_15235"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3013895.1"
                     /translation="MSIKSDRWIRRMARERRMIDPFEPNQIKEVKGRKVVSFGTSSYG
                     YDIRCSDEFKLFTNINTTIVDPKNFDPNSFVDLKSEVCIIPPNSFALARTVEYFRIPR
                     NVIVLTLGKSTYARCGIIVNVTPLEPEWEGYVTLEFSNTTPLPAKIYANEGVAQVIFL
                     EAQPDDVCETSYKDRGGKYQGQRGVTPPKV"
     gene            4404..5600
                     /locus_tag="AC16CX_15240"
     CDS             4404..5600
                     /locus_tag="AC16CX_15240"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HIR3013896.1"
                     /translation="MAERPVALKNQNSQAAPETEQSQLFDTHPEVRLDFKHVRKALKQ
                     GYTRPFLLVDASIVRTKVRRFKAAMPRVHPHYAVKANPDPRVLKVLIEEGAGFEIASI
                     AELDLLLELGVPAAEVYYSNPMKSRAYIEYAAAKGVEWFALDSVEELRKIHSIKPDAK
                     LYVRIEAPNVGSDWPLSGKFGMKAQEVDAIVAEAVELKADLAGVTFHVGSQCRNPENW
                     RVGIQGAKRVFKKMRLAGLKPRLLNIGGGFPVRHVKPIPSIEKIAEVVNHALRDVPQS
                     VRVMAEPGRYFVSDAGYFVCRVVGTATRSGKRWMYWDAGVFGGIIETTEGLRYDVQTD
                     RSGKLIPWNVAGPTCDSVDVCMRDEMFPEDLREGDFIYIVNAGAYTTAYASNFNGFPL
                     PEVRVI"
     gene            complement(5775..6470)
                     /locus_tag="AC16CX_15245"
     CDS             complement(5775..6470)
                     /locus_tag="AC16CX_15245"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013897.1"
                     /translation="MNPKLGFACLLLVLSCAGFALAQDKPAQGKVSVEDLKIRSEAGD
                     RTATRQLAEMYYLGRDGVEQNFSEAARWYLKLAKQGDVRAQTTIGLMYSRGYGVNKDP
                     AAAHRWWNFAAAQNDPGAQYNLGLSYAQGDGVTQDYERAARWYNQAASRGHIQAQHNL
                     GMLYHEGKGVERDPLRAYYWLKVAALQGDDVSQDALMLVGKGMSADQIRQAEVQADEW
                     LKKYKKILGKPDS"
     gene            complement(6586..7065)
                     /locus_tag="AC16CX_15250"
     CDS             complement(6586..7065)
                     /locus_tag="AC16CX_15250"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR3013898.1"
                     /translation="MIVAMAKNRVIGANGIIPWHLPEELKRFKRLTLGHHLIMGRKTW
                     ESIGRPLPGRASVVVTRQRDYRAHGALVMHSLDDAIAACAGDSEIFVIGGAELYAQAL
                     PRADRLYLTVVDAKIAGDTVMPEFDLSGWREVSSESFAADARHRYPFRCVTYERVMK"
     gene            complement(7155..7949)
                     /locus_tag="AC16CX_15255"
     CDS             complement(7155..7949)
                     /locus_tag="AC16CX_15255"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR3013899.1"
                     /translation="MRQYLDLMRHVLEHGAHKNDRTGTGTLSVFGAQLRFDLNAGFPL
                     LTTKKVHLKSIIHELLWFLKGDTTIRYLKENGVTIWNEWADKNGELGPVYGYQWRSWP
                     APDGRHIDQIGEVIAQIRKTPDSRRLIVSAWNVADLPRMALLPCHAFFQFYVADGRLS
                     CQLYQRSADIFLGVPFNIASYALLTLMVAQVCGLKVGDFVHTFGDTHLYLNHLDQARE
                     QLSREPRRLPVMKLNPAVRNLFEFKYEDFTLENYDPHPAIRAPVAV"
     gene            complement(8010..8795)
                     /locus_tag="AC16CX_15260"
     CDS             complement(8010..8795)
                     /locus_tag="AC16CX_15260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013900.1"
                     /translation="MQRLLVALALFLPLGAPSAEDIVTLSTRGGMTQSYLLSAPDAGK
                     ARAVAILFPGGAGKTDLERETARRALDRGNFLVRSRRLFADRGIAAAVVDAPSDQPRG
                     MEDEFRLGAAHSEDIGRVVADLKTRFPALPVFLVGTSRGTVSAASIGARLGGAVDGIV
                     LTSTLFLASRRGPGLSGFDFSSVPSPLLFLHHVDDACAYTPHASAKRLADRYPLVSVA
                     GGLPPQSQPCEAMSAHGFLGKEAEAVDAIAKWMLKQPYPKEIN"
     gene            8923..10395
                     /locus_tag="AC16CX_15265"
     CDS             8923..10395
                     /locus_tag="AC16CX_15265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HIR3013901.1"
                     /translation="MLARLEQNAALFTAADLKRAKHALIVLPKAESLESLRGVPGMEA
                     LSAALSRRKKKLADLSKTPVVTDLTHGTLVSWALLDPARSVFEQQTTLRKGLQLLLSE
                     KPGEIAIGVFGTAAARRHAAELAVYAAWVNGARLPERKKKPNNAALRILRLYGHRSAD
                     GFSWQRAVAEGNLLCRELTVLPPNELTPGSYRKRVGQIAKQHGWRREEYDSKRLRRMG
                     AGAFAAVAQGSAEDDAAIVHLRYRHPRAKKSFTLVGKGICFDTGGHNLKPARSMHGMH
                     KDMNGSAVALGILLAAAEIRLPLQLDCWMAIARNDISPRAYRQNEVVTALDGTTIEIV
                     HTDAEGRMVLADALALAARAKPDLIVDFATLTGSMHVAVGERYSGVFATSDELARQAV
                     AAGKASGERVCAFPMDEDYDEALESEIADVKQCTMEGEADHILATRFLKRFTGDRSWV
                     HMDLSAARCKGGLGAVATDINGFGVAWGVEFLKAALGRPH"
     gene            10515..12401
                     /locus_tag="AC16CX_15270"
     CDS             10515..12401
                     /locus_tag="AC16CX_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3013902.1"
                     /translation="MQERSQFRLLLERRFAPFFGVQFLGALNDNVFKQALVILLAYQT
                     ASFTTLSSDVLQNVAQALFILPFFLFSATAGQFADKYDKSRLITVTVFIELCVMALGA
                     VGFFLRNLELLLLALFLGGVQSALFGPVKYAILPQHLRETELIGGNGLIEMGTSIAIL
                     LGMVLGGWMVSQAGWGIAAVAFATMAISATGLLLSRYIPLAPAADPGLRINWNLAGET
                     WRNFQFMRGNRTVFLSILGISWFWFFGAMFVTQFPNLSKNVLSGDEHIVTLLLVVFSF
                     GIGAGSLLCERLSGHKVEIGLVPFGSIGLTLFAVDLYFALADHIQHGPVGPGEFIREA
                     GHWRILGDLFLIGLFGGFYIVPLYALIQIRSEVSHRSRVIAGNNILNALFMVVAALLA
                     IGMFQRDFTIPQLFLMTALLNAAVAVYIYTLVPEFLMRFLVWLLIHSVYRLEKSGLEN
                     IPEEGPAVLVCNHVSFVDALIIAAACHRPIRFVMDHRIFRIPVLNFVFRTGRAIPIAS
                     SKEDPQLLERAYDEIARALQQGDLIGIFPEGRITDTGEMHPFHSGIQRIIARTPVPAV
                     PMALRGLWGSFFSRMGGAAMTRPFRRGVFSKIALAVAPAIPPQQVSPEGLQETILALR
                     GDWK"
BASE COUNT         2348 a         3878 c         3925 g         2408 t
ORIGIN      
        1 tgccggctaa cacggtcctg gttccgtcac ccagatcgag cgtgagcctc aagagcttgt
       61 ccgcgccttc cacgggttcc gcgttcacga tcttcgcgac gcggaggtcc accttgttga
      121 agtcgtcaat cgttatctgc gcgcctgcgc ctgtactctt cccctcgccc tcagggagag
      181 ggggaagggg tgagggtggg gtttgcgtgc tcactgggcc tcctgctttc tttgctgcgt
      241 gcgcctgatg ctcagcatgg cgctgttgtg aatggggctg cggcgccggt tgcaggcttt
      301 ccttgctggc ttccaccagg gtggtgatct gcttcgggtc gatgcgggtc atcaaatgga
      361 cgtaaggctt ggttttgtgt ccctgttcta gatgcttttg tgcgtcgtcc catttgagtt
      421 ccgagatccc aagcatttgc tccacgtgcg aagctaggtt tggtaagact ggcttcagat
      481 aaatcgtcag gagtcgaaaa gcgtcaatgg tgcaagagca aactttgtgc agttctgaat
      541 gctgattggg attcttggca atttcccaag gcttcttgtg atcaatatag acgttgatgt
      601 agtcggcgag catcatgatc tctcggacag ccttcccaaa ctccctgtct tcatagagtt
      661 ccttgattcg cggactagat ttttgtacgg tggcgagcaa tgcgtcagct gttgctgatt
      721 ctgcacgcag gtaagcgaga aagtctttat gttcaccttc atcggcagat cggtcagcgc
      781 cagcgatagc acgagcaaga cgagcgtcaa attctgtagg gtttgcaagt gaagacccaa
      841 tctttccctc gaaatacttg gttagaaaag gaagagtgcg gctcgcgata ttgatgtact
      901 tgccgaccag gtcgctgttg acccgagccg caaagtcgtc gaggttgagg tcgatatccc
      961 ccatcgtggg gcccagtttg gcggcatagt aatagcgcag ccactcgggg ttgaggcctt
     1021 gcttaaggta gctttcggcg gtgatgaagg tgccacgcga cttggacatc ttctcgccgt
     1081 tcaccgtcaa aaagccgtgt acgtacaagt gagagggcgc gcggtagccg gcgaattcga
     1141 gcatcgccgg ccagaacagg gcgtggaagt agaggatgtc cttgccgatg aagtggatca
     1201 tctcggtctt cgcgccggcc ttggtgaacg cgtcgaactc gatcttcttg ttgcggacgg
     1261 cgaggttctt gagactgccg aaatacccga tcggcgcatc gagccagacg tagaagtact
     1321 tgccgggcgc gcccgggatc tcgaaaccga agtagggcgc gtcgcgcgag atgtcccaat
     1381 cgtttagccc ctggtcgaac cattcgcgga tcttgttccg cgcttcaggc tgcaggtgct
     1441 cgtcttcctg tgtccactgc tccaggaact tcctgcagcg cggatcggag agcttgaaga
     1501 aatagtggtc ggatgtcttg cgcaccggct tcgcgcctga caccaccgag tacgggttct
     1561 tgagatcggt cggactgtaa gtcgagctgc agttctcgca cgcgtccccg tactggtccc
     1621 tggcgccgca tttcggacac tcgcccttga tgaagcggtc gggcaggaac aggttcttca
     1681 ccgggtcgaa gaattgctcc accggcttct tgtggatcag gccggcggca tccagcttcc
     1741 tgtagaactc ttccgaaagc gtccgggtct catccgagtg agtggtgtag tagttgtcga
     1801 actcgacgtg gaaccccttg aagtcgcgct tgtgctcctc cagcacgcgc gcgatcagcg
     1861 cctccggcgt gatgccgtcc tgctcggcgc gcagcatgat cgccgtgccg tgggtatcgt
     1921 cggcgcagca gtaatgcacc tcgtggcccc gcatccgctg gaagcgcacc cagatgtcgg
     1981 tctggatgta ctccaccagg tggccgaggt ggatcgcccc gttggcgtag ggcagggccg
     2041 aggtgagaaa aatactgcgt ggcataagcg tcgaattgta accgataaat ccttgctgcc
     2101 agaatggttt agccgtggtt tgataggcgc ggcgcgcatt atcgtctaga atcgcgccct
     2161 acacgccaac gagacatttc tcatggtcac cgagcagcaa gtccacgatg tcctgaaaca
     2221 gctcaccgat cccaacaccc ggaaggactt cgtctccacc aagtccgcgc gcaacatcaa
     2281 ggtggacggc gacaaggtgt cgttggagat cctgctgggt tatcccgcga agagccagtt
     2341 ggcgccgatc cgggaggcgg tcactcaagc gctcagggcg atcccgaacg tcgccggcgt
     2401 caacgtcaac gtacaaatga agatcgtctc gcatgcggtc cagcggggcg tcaagctcgt
     2461 gcccggcatc aagaacatca tcgcggtggc ttccggcaag ggcggcgttg gcaagagcac
     2521 ggtggcggtc aacctggcgc tggcgcttgc cgccgagggg gcttcggtcg cggtgctgga
     2581 cgcggacatc tacggcccct cccaacctac catgctcggc atccgcggcc ggcctgagtc
     2641 gcgcgacggc aagaccctgg agcccatgga aggccacggc gtgcaggcga tgtccatcgg
     2701 cttcctgatc gacctcgaga ccccgatggt ctggcgcggg ccgatggtga cccaggcgct
     2761 ggagcagctc ctgaacgaaa ccaagtggcg cgaggtggat tacctggtgg tggacctgcc
     2821 gccgggcacc ggcgatatcc agctgacgct cgcccagcgc gtgccggtga cgggcgccat
     2881 tatcgtcacc acgccccagg acatcgccct catagacgcc cgcaaggggc tcaagatgtt
     2941 cgagaaagtg ggcatcccca tcctcggcgt ggtcgagaac atgagcctgc acatctgctc
     3001 caagtgcggg cacgaagaac ggattttcgg cgaggggggc ggggagcgca tgagcaagga
     3061 ttacaacgtg gaaatgctcg gcgccctgcc gctcgacatc cgcattcgcg agcaggccga
     3121 ttccggaaag ccgacggtgg tctccgatcc cgacgggcga gtcgcccaga tctacaagca
     3181 gatcgcgcgc cgtgtggcgg tgaagatcgc cgagaaagcg caggaccaca cggcggcgtt
     3241 cccgaagatc gtcattcaaa acacgtgagg ggagagaggt gaggagagag gagtaaaatc
     3301 cggcgcaaac aacgacccct cttcactcct tatcccttac ccctcactcc tcaccgcgaa
     3361 gcatgagcat caaatccgac cgctggatcc ggcgcatggc gcgcgagcgc cgcatgatcg
     3421 atccgttcga gccgaaccag atcaaggaag tgaaagggcg gaaagtggtg tcgttcggca
     3481 cctcgagcta tggctacgac atccgctgct cggacgaatt caagcttttc accaacatca
     3541 acaccaccat cgtcgatccc aagaacttcg accccaactc gttcgtggac ctgaaaagcg
     3601 aggtctgcat catcccgccc aactccttcg cgctcgcccg caccgtcgaa tacttccgca
     3661 tcccccgaaa cgtcatcgtc ctgactctcg ggaagtcgac ctacgcccgc tgcgggatca
     3721 tcgtcaacgt cacccccctc gagccggagt gggagggcta cgtgaccctc gagttctcga
     3781 acaccacccc cctgccggcc aagatctacg ccaacgaggg agtggcgcag gtgatcttcc
     3841 tggaagccca gccggacgac gtctgcgaga cctcctataa ggaccgcggc ggcaagtacc
     3901 agggccagcg aggcgtcacg ccccccaaag tctgataaac cggacgccaa gcgccggttt
     3961 gtctccgcgc attggagcgg acagccggcc tgccgctcac cgcgtggtcg aagaatccga
     4021 gaagcgggcc tgaggcacca gtcatcactc gcaggttagg ggtcgtcact cacgtttctc
     4081 caggctcgtg gcgacgacgc gtcgcggctg tccgaaactc ttcttccgat ctacctcggc
     4141 ttccatcgag ctccactttg gcgccgagcg acggctcgcg accgttgcgt atgtgacacg
     4201 cgcgccgaaa aattttccga aaaccggaaa aaaactcttg attcctggta cagcggccat
     4261 ataattcgcg gaagttttac atttttgtga cgcgcgctgg ggtgttgaag gaaacgagca
     4321 tgacaacggc aagcaaaagc cgcctgacgg cggaagtggt tgtccgagac gtgccggaaa
     4381 cctaggtctc aaggagaact ttgatggccg agcgccctgt cgccctcaag aatcagaatt
     4441 cacaagccgc ccccgaaacc gaacagtccc aactcttcga cacgcatccc gaagtccgcc
     4501 tggacttcaa gcacgtccgc aaagcgctca aacagggcta caccaggccc ttcctgctgg
     4561 tggacgccag catcgtccgc accaaggtcc gtcgcttcaa agccgccatg ccgcgggtcc
     4621 atccgcatta cgcggtcaag gccaaccccg acccccgcgt gctcaaggtc ttgatcgagg
     4681 agggggccgg cttcgaaata gcctcaatcg ctgagcttga cctcttgctc gagctggggg
     4741 tgccggccgc cgaggtctat tacagcaacc cgatgaagtc gcgggcctat atcgaatatg
     4801 cggcggcgaa gggtgtcgaa tggttcgcgc tggacagcgt tgaggagctg cgcaagatcc
     4861 acagcatcaa gcccgacgcc aagctctacg tccgcatcga ggcccccaac gtaggcagcg
     4921 actggccgct gtcgggcaag ttcgggatga aggcgcagga ggtggatgcc atcgtcgccg
     4981 aggcggtcga gctcaaggcc gacctcgccg gggtgacctt ccatgtcggc tcccaatgcc
     5041 gcaatccgga gaactggcgt gttggcatcc agggcgcgaa gcgggtgttc aagaagatgc
     5101 ggctcgccgg cctcaagccc cggctgctca acatcggcgg cggcttcccg gtgcgccacg
     5161 tcaagcccat cccctcgatc gagaaaatcg cggaagtggt gaaccatgcg ctacgcgacg
     5221 tgccccaaag cgtgcgggtg atggccgagc ccggccgcta cttcgtgtcg gacgcgggct
     5281 acttcgtctg ccgcgtcgtc ggcaccgcga cccgctcggg caagcgctgg atgtactggg
     5341 acgcgggggt gttcgggggc atcatcgaga ctaccgaagg gctgcgctac gacgtgcaga
     5401 ccgaccgcag cggcaagctc attccatgga acgtcgcggg cccgacctgc gactcggtgg
     5461 acgtgtgcat gcgcgatgag atgttccccg aggacctgcg ggaaggcgat ttcatctaca
     5521 tcgtcaacgc cggcgcctac accacggcct acgcgagcaa cttcaacggc tttccgcttc
     5581 cagaagtacg cgtgatttag ccgcgccagg tcttgacaga acggcgagtc ctggcgactc
     5641 gttttttttc gtctgcgcgg ccgcacggtc tgtttgacac gcacgtaatg acacgtcgac
     5701 acgcgttttt gttgcgaaaa tgcaacattg tcaaaagatc gagcggccgg ggtaacccgg
     5761 ccgttttttt gcggctacga atcgggcttg cctagaatct tcttgtactt cttcaaccac
     5821 tcatcggcct ggacttccgc ctgccgaatc tgatccgcgc tcatgccctt gccgacgagc
     5881 atcagcgcgt cctgtgacac gtcgtcgccc tgcaacgccg cgaccttgag ccagtagtag
     5941 gcacgcagcg ggtcgcgctc gacgcctttg ccctcgtggt agagcatgcc gagattgtgc
     6001 tgcgcctgga tatggccgcg gctcgcggcc tggttgtacc atcgcgcggc ccgctcatag
     6061 tcctgcgtga cgccgtcgcc ctgagcgtag gagagcccga ggttgtactg cgcgccggga
     6121 tcattctggg cggcggcgaa gttccaccag cggtgggcgg ccgcggggtc cttattcacc
     6181 ccgtagccac gcgaatacat caacccgatg gtggtctgcg cgcgcacatc tccctgcttg
     6241 gcgagcttga ggtaccagcg cgccgcctcg ctgaaattct gctcgacgcc gtcccggcca
     6301 aggtagtaca tctcggcaag ctggcgcgtc gcggtcctgt cgcccgcctc ggagcggatc
     6361 ttgaggtcct cgacgctgac cttgccctgg gctggcttgt cctgcgccag cgcgaaaccc
     6421 gcgcagctca aaacaagcaa tagacaggca aaacccagtt tgggattcat cgctaaaaaa
     6481 tggacaggat tcacaagagt aaagttctgt aataccgaat agacaggatt tacatgatta
     6541 acaggattaa ccccgctctc taatcttgta aatccttaat cctgtctatt tcattacgcg
     6601 ctcataggtc acgcagcgga acggataccg gtgccgcgcg tcggcggcga atgattcgga
     6661 ggatacctcg cgccagcccg acagatcgaa ctccggcatg acggtgtcgc ccgcgatctt
     6721 ggcatcgacc accgtgagat acaggcggtc ggcgcgcggc agcgcctgcg cgtagagctc
     6781 ggccccaccg atcacgaaga tctcgctgtc acccgcgcac gccgcaatgg cgtcatcgag
     6841 cgaatgcatg accagcgctc cgtgcgcccg gtaatcgcgc tggcgcgtga cgacgaccga
     6901 tgcgcgcccc ggcagcggcc gcccaatcga ttcccaggtt ttccggccca tgatgaggtg
     6961 gtggcccagg gtgaggcgct tgaaccgctt cagctcctcc ggaagatgcc agggaatgat
     7021 gccgttcgcg ccgatcacgc ggttcttggc catggccact attatcgaaa ggcgtggatc
     7081 gtgggtcgtg ggtcgtggat cgtcccgcga agcgcgcggc tttttctcct cactcttcac
     7141 ccctcacccc tcactcacac cgcgaccgga gcccggatcg cggggtgagg atcgtagttc
     7201 tcgagggtga aatcctcgta cttgaactcg aagaggttcc gcaccgcggg gttgagcttc
     7261 atcaccggca agcggcgggg ctcacgcgag agctgctcgc gcgcctggtc gaggtggttg
     7321 aggtagagat gggtatcacc gaaagtgtgg acgaagtcgc cgaccttgag cccgcacacc
     7381 tgcgccacca tcagcgtgag cagcgcatag gaggcgatgt tgaacggcac gccgagaaag
     7441 atatcggcgc tcctctggta gagctggcac gaaagccttc catcggcgac gtagaactgg
     7501 aagaaggcat ggcacggcag cagcgccatt ctgggcaggt cggcgacgtt ccaggccgag
     7561 acgatcaggc gccgggagtc gggggttttc cggatctgcg cgatcacctc gccgatctgg
     7621 tctatatgac gcccgtcggg cgcaggccac gagcgccact ggtagccata aacgggtccc
     7681 agctcgccgt tcttgtccgc ccactcgttc cagatggtga cgccgttttc cttcaggtag
     7741 cggatggtgg tgtcgccctt caggaaccac agcagctcgt ggatgatgga cttgagatgc
     7801 accttcttgg tggtgagcag cgggaacccc gcgttgaggt cgaagcgaag ttgcgccccg
     7861 aagacggaaa gcgtgccggt gccggtgcgg tcgttcttgt gcgcgccgtg ttccagcacg
     7921 tggcgcatca ggtcgaggta ttggcgcatg acttcaggat tgaggattga gcaatgagta
     7981 ttgagcagaa ccgatggcgt ttgctgattt tagttgattt ccttcggata gggctgcttc
     8041 agcatccact tggcgatcgc atccaccgct tccgcttcct tgccgaggaa accgtgcgcg
     8101 ctcatcgcct cgcagggctg ggactgcggc ggcaggccgc cggcaaccga caccagcggg
     8161 taacgatcgg cgaggcgttt cgccgatgcg tggggcgtgt aggcgcacgc atcatccacg
     8221 tggtgcagga acagcagcgg ggacggaacg gaggaaaagt cgaacccgct caaccccggc
     8281 ccgcgcctgc tggcgaggaa gagggttgaa gtcagcacga tgccgtccac cgcgcctccg
     8341 agacgtgccc cgattgacgc agcggatacc gtgccgcggc tcgtgcccac gaggaacacg
     8401 gggagggcgg ggaagcgggt tttcaggtcc gccaccaccc gcccgatgtc ttcggagtgt
     8461 gcggccccca accggaactc gtcctccatg ccgcgcggct ggtcggacgg cgcatccaca
     8521 acggcggcgg cgataccgcg gtccgcaaac agccggcgcg agcgcacgag gaaattgccg
     8581 cgatcgagcg ccctgcgcgc ggtctcgcgc tcgagatcgg tcttgcccgc gccgcccgga
     8641 aacaggatcg ccacggcgcg cgccttgccc gcatccgggg ccgacaacag ataggattgc
     8701 gtcatgcccc cgcgcgtgga cagggtcacg atgtcctcgg ctgacggcgc gccgaggggg
     8761 agaaacaacg caagcgcaac gagcagtcgt tgcatttgca aaattatagt cccgccgcgc
     8821 cgtgatatta aaaaaccctg ctcgacagcg catgcgggta gagcggggtt ttttgcggtt
     8881 tataatccgc cttcttcccg atcaatgact tgtggactcc tgatgctggc gaggctcgag
     8941 cagaacgcgg cgctttttac agcggcagat ctgaaacgcg cgaagcacgc cctcatcgtg
     9001 ctgccgaaag cggagtcgct cgagtcattg cgcggcgtgc ccggcatgga ggcgctttcc
     9061 gccgccctct cgaggcggaa gaaaaagctc gccgacttgt ccaagacgcc agttgtcacc
     9121 gacctcacgc atggaacgct ggtgtcctgg gccctgctcg atccggcgcg atcggtgttc
     9181 gagcaacaaa ccactctgcg caagggcctg caattgctgc tctccgaaaa gccgggggaa
     9241 atcgcgatcg gggtgttcgg aaccgccgcc gcgcgccgcc atgccgcgga actcgccgtc
     9301 tatgcggcgt gggtcaacgg cgcccgactg cccgagcgca agaagaagcc gaacaatgca
     9361 gcgcttcgga tcttaagatt gtatgggcac cgttcggctg acggtttttc ctggcaacgg
     9421 gcggtcgcgg aaggaaacct gctctgccgg gagctcacgg tgctgccgcc caacgagctg
     9481 acgccgggaa gttaccgcaa gcgggtgggg cagatcgcca aacagcacgg ctggcggcgc
     9541 gaggaatacg actcgaaacg gctgcgccgg atgggcgctg gcgcgttcgc cgccgtagcg
     9601 cagggcagcg ccgaggacga cgccgcgatc gtgcacctgc gctatcgcca cccgcgggca
     9661 aaaaagtcct tcaccctggt cggcaagggc atctgcttcg acaccggcgg ccacaacctc
     9721 aagccagccc gctccatgca cggcatgcac aaggacatga acggctctgc ggtcgcgctc
     9781 ggcatcctgc tcgcggcagc ggagatcagg ctgccgctcc aactcgactg ctggatggcg
     9841 atcgcgcgca acgacatcag cccccgcgcc tatcgtcaga acgaagtggt cacggcgctc
     9901 gacggcacca ccatcgaaat cgtccacacc gacgccgaag ggcgcatggt gcttgcggac
     9961 gcgctcgcgc tcgcggcgcg cgcaaagcct gatctcatcg tggatttcgc gacgctcacg
    10021 ggcagcatgc acgtcgcggt cggcgagcgc tacagcggcg tgttcgccac cagcgacgag
    10081 cttgcgcggc aggcggtggc tgcggggaag gcgagcggcg agcgggtgtg cgccttcccg
    10141 atggacgaag actacgacga ggcgcttgag agcgagattg ccgacgtgaa gcagtgcacc
    10201 atggaaggcg aggcggacca tattctcgcc acgcgcttcc tcaagcgctt cacgggagac
    10261 cgctcatggg tgcacatgga cctgtcggcc gccagatgca agggcgggct cggcgcggtc
    10321 gcgaccgaca ttaatggttt cggcgtcgcc tggggtgtcg agtttctgaa ggcggcgctg
    10381 ggccggccac attaaactgt gttctcataa catctcgaaa gttattgttc actgagctct
    10441 ttaaagcgtg cgctcgcgcg ccgctttgat tgcgcgatat ccaatctttc tcgcgccgga
    10501 gggaggggag gaagatgcag gaacgcagcc agttccgcct gctgctggag cgccgcttcg
    10561 cgccgttctt cggcgtgcag ttcctcggcg cgctcaacga caacgtcttc aagcaggcgc
    10621 tggtcatcct gctggcctac cagacggcga gcttcaccac cctgtcgagc gacgtgctgc
    10681 agaacgtcgc gcaggcgctg ttcatcctgc cgttcttcct gttttccgcg accgccggcc
    10741 agttcgccga caaatacgac aagtcccgcc tgatcaccgt cacggtattc attgagctgt
    10801 gcgtcatggc gctcggcgcg gtgggtttct tcctgcgcaa cttggagctg ctgttgctgg
    10861 cgctctttct gggcggggtg cagtcggcgc tgttcggccc ggtcaagtac gcgatcctcc
    10921 cccagcacct cagggaaacc gagctgatcg gcggcaacgg cctgatcgag atgggaacgt
    10981 cgatcgccat acttctcggc atggtgctcg gcggctggat ggtgagccag gcggggtggg
    11041 gcattgcagc ggtcgccttc gcgaccatgg caatttccgc caccggcctg ttgctgagcc
    11101 gttacatacc actcgcgccc gcggcggacc cgggtctccg aatcaactgg aacctggccg
    11161 gcgagacttg gcgcaacttc cagttcatgc gcgggaaccg gaccgtgttc ctctcgatat
    11221 tgggtatctc gtggttctgg tttttcggcg cgatgttcgt cacgcagttc cctaacctga
    11281 gcaagaacgt tctgtcaggg gacgaacaca tcgtcacgtt gttgctggtg gtgttctcct
    11341 tcggcatcgg cgcgggatcg ctgctgtgcg agcggctgtc cggccacaag gtcgagatcg
    11401 gtctcgtgcc gttcggttcc attggtctga ccctattcgc ggtcgacctg tatttcgcgc
    11461 tggccgacca catccagcat gggcccgtgg gcccgggcga attcattcgg gaagccgggc
    11521 actggcgcat cctcggcgac ttgttcctga tcggactgtt cggcggcttc tacatcgtgc
    11581 cgctctatgc gttgatacaa atccgcagcg aggtcagcca ccgctcgcgg gtcatcgccg
    11641 gcaacaacat cctcaacgcg ctgttcatgg tggtggcggc gctgctcgcg atcgggatgt
    11701 tccagcgtga cttcaccata ccgcaactgt tcttgatgac ggcgctgctc aacgcagcgg
    11761 tggcggtcta catctacacg ctggtgccgg aattcctgat gcggttcctc gtgtggcttc
    11821 tgatccactc ggtctaccgc ctcgaaaaga gcgggctcga gaacattccc gaggaaggcc
    11881 cggcggtgct ggtgtgcaac cacgtgagct ttgtggatgc gctgatcatc gccgcagcat
    11941 gccaccgccc gatccgtttc gtgatggacc accgcatctt caggatcccg gttctgaatt
    12001 tcgtgttccg gacgggacgc gccatcccga tcgcgtcgtc gaaggaagac ccacagttgc
    12061 tggaacgcgc gtacgacgag atcgcgcggg cgctgcagca gggggacctg atcggtatat
    12121 tcccggaagg ccggattacc gataccgggg agatgcaccc cttccactcg ggcatccagc
    12181 gcatcatcgc gcgcactccg gtgcccgccg tgccaatggc gctgcgcggt ctgtggggca
    12241 gcttcttcag ccggatggga ggggctgcga tgacgcggcc gttccggcgc ggcgtgttct
    12301 caaagatcgc attagcggtg gctccagcga tcccgccgca gcaggtgagc ccggagggac
    12361 tgcaggaaac aatcctggct ctccgtggcg actggaaata acccgcacgt cgaggttact
    12421 cctgtttatc atcatcaaaa atcgtggacg aaacttagcc tcggccacga tttttgtccc
    12481 acacgtagcg cacgaagcgc tgccagcggc tctgcggctg cgcgagcttg ttgtgcacct
    12541 tgctcgccgc ctggtcgcg
//
LOCUS       DBNGWZ010000495         3572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2582756, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000495 DBNGWZ010000000
VERSION     DBNGWZ010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2582756"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1277)
                     /locus_tag="AC16CX_15275"
     CDS             complement(48..1277)
                     /locus_tag="AC16CX_15275"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3013903.1"
                     /translation="MSDERSNFMVVWDPVIPRPELISRIRAALARSRVVALVGPRQCG
                     KTTLARALAHVGRANYFDLEDPRSLARLVEPMTALEHLRGLVVIDEVQLRPDLFPVLR
                     VLADRRPLPARFLVLGSASPALLKQSTESLAGRIEIIDMGGFSLAEVGARQVERHWLR
                     GGFPLSYTARTVAASVAWRTQFVRSFLERDLPQLGITIPAQALRRFWIMLAHYHGQIW
                     NAAEPARSLGVAETTARRYLDLLTGFYMARQVAPWHENLGKRQVKSPKVYVRDSGLLH
                     NLLGITNEDELLAHPKCGASWEGYAIEEVVNAVKPDDVYYWATHQGAELDLLLFKNGR
                     RLGVEVKRMDAPRLTPSMRIALEDLKLEQLVVFYPGSDEYALSERVRVTPLVKLATVD
                     ADTLFPESGPPKAALGT"
     gene            complement(1524..2708)
                     /locus_tag="AC16CX_15280"
     CDS             complement(1524..2708)
                     /locus_tag="AC16CX_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HIR3013904.1"
                     /translation="MNKKAMAIAVAGVLAAPSAAVLAQTSTVQIGGSITTFYYSHSPK
                     NSSSGQRTDILETSEPEMFVRGEEKLGGGLSAWFQCTSSLDAMVTGAVDAGGFCGRNS
                     GIGLKGSFGNLFQGNWDTPDKLVYNNARQWFGGTNSFTGGSARLLLGGSASGQGNSVA
                     GSNTPSSFFRRQASSWNYHSPSWNGFTVNGAFSAANEQTGIPEASPLTPRMWSLGANY
                     SSGPLYLGLGYERHNDYNPSNQAAALYTGGTDDNWTLVAGYRWGGFSVRGAYVRSEYD
                     TTNTTQLKVRGFALFGDWNIQGPHTLRAQYADVSDMKGNSAVSVSVYRPPMVGGVGVT
                     DTGAKLWSIFYSYAFSKRTEGSIGYTKLKNDRNAGFTLGKVNASTGNNQTSAGIVFKH
                     RF"
     gene            3014..>3572
                     /locus_tag="AC16CX_15285"
     CDS             3014..>3572
                     /locus_tag="AC16CX_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3013905.1"
                     /translation="MSKGEIGIQNLIQAGLRAHHAGRLEEAKALYEQALAIRPRHPDA
                     LHLAGVVALQAGEAERAVALIQKAIKVQPDNPGFHANLAQAYLASRRFEDARAAFRRA
                     ARLEPRDPQFAVGAANCLAMQGSVAEAERELHGVTQRHYSYALAWFNLGNVVQEQGRQ
                     QEAEEYYRRAIELDPAFADAHTNLGG"
BASE COUNT          726 a         1098 c         1066 g          682 t
ORIGIN      
        1 aaacggtaag cggtaagcgg tgagcagtaa ttagaacccg cttgctctca agttccaagc
       61 gcagccttcg gcgggcctga ctccgggaac aacgtatccg cgtcgacagt cgccagcttg
      121 actagaggcg tgacgcgaac acgctcggaa agagcgtatt cgtccgagcc tggatagaac
      181 acgacgagct gctccagttt cagatcctcc aatgcgatcc gcatggaagg cgtcagtctt
      241 ggagcatcca ttcgcttcac ttccaccccg agcctgcgac cattcttgaa cagcaacaga
      301 tccagttccg cgccctgatg cgtggcccag taatacacgt cgtcaggttt caccgcgttg
      361 acgacttctt caatagcgta gccctcccag gacgcgccac atttggggtg agcgagaagc
      421 tcgtcctcgt tggttattcc gaggagatta tggagcaacc cagagtcgcg cacgtacacc
      481 ttcggggact tcacctgccg cttcccgagg ttctcatgcc agggcgcgac ctgacgcgcc
      541 atgtagaagc cggttaacag gtccagataa cgcctcgccg tggtctcggc cacaccgaga
      601 gaacgcgccg gctctgccgc gttccagatc tggccgtgat aatgcgcgag cattatccag
      661 aatcgcctca atgcctgtgc cggaatggtg ataccgagct gcggcaaatc gcgttccagg
      721 aacgaacgca cgaactgggt tcgccacgca acgctggcag ccacggtgcg cgcggtatag
      781 ctcaacggaa atccgcctct taaccaatgg cgttccactt gccgcgcgcc gacctccgcg
      841 agagaaaacc ctcccatatc aataatctcg atccttccgg cgagggactc ggttgactgc
      901 ttcagcagcg ccggcgacgc gcttcccagc accagaaacc gcgcgggcag cggacgccgg
      961 tcggccagca ccctgagaac gggaaaaaga tcgggacgaa gctgcacttc gtcgatcacg
     1021 accaggcccc tcaggtgttc gagggccgtc atgggttcga caagccgtgc caggctgcgg
     1081 ggatcttcca ggtcgaagta attcgcgcgt ccgacgtgcg ccagtgcccg ggcgagggtg
     1141 gtcttgccac actggcgggg gccgaccagg gccacgaccc ggctgcgggc gagcgcggcg
     1201 cggatacgcg aaatcagctc aggtcggggg atcacgggat cccatactac catgaaattc
     1261 gagcgctcat cgctcaattt tcatggtctc ttacttcttt tccctgctcg tcgcgccttt
     1321 tggccttatg ccttgttgtt gagcagatcc cgggaaggga gttccagatt cattaggagc
     1381 aaggtgcagc tcggaaacca tccccaccca cggataacca cggacagcgg tagtaaggaa
     1441 tttggcggac actagtccgg caaaatcctc ggggcaaaaa aaacgggcac ccgaaggcgc
     1501 ccgtttcaag cctgctgtta cggttagaag cggtgcttga acacgatgcc cgcgctggtc
     1561 tggttgttgc ccgtcgaagc atttaccttg cccagagtga aacccgcgtt gcggtcgttt
     1621 ttcagcttgg tatagccgat cgaaccctcg gtccgcttgg agaacgcgta ggagtagaag
     1681 atcgaccaca gcttcgcacc ggtgtcggtt actccaaccc cacccaccat cgggggccgg
     1741 tagacgctga cgctcaccgc cgaattgccc ttcatgtcgc tgacgtcggc gtactgcgcc
     1801 cttaaggtat gcggcccctg gatgttccag tcgccgaata gagcgaagcc acgcaccttg
     1861 agctgggtgg tattggtggt gtcgtattcg ctccggacgt acgcgcccct gacgctgaaa
     1921 ccgccccacc gatagccggc gaccagcgtc cagttatcgt ccgtgccccc tgtgtagagg
     1981 gcagctgctt gattggacgg gttgtagtca ttatggcgct cgtagccgag gcccaggtac
     2041 agcggtccac tgctgtagtt cgcgccaagg ctccacatgc gcggggtcaa cgggctggct
     2101 tcgggtatgc cggtctgctc gttggctgcg ctgaacgcgc cgttaactgt gaagccgttc
     2161 caggacgggc tgtgataatt ccacgaggat gcctgccggc ggaagaagct cgacggggtg
     2221 ttcgagccag ctaccgaatt cccctgcccg ctggccgagc cgccgagcag caaccgggca
     2281 gaaccgccgg taaacgagtt cgtgccgccg aaccactggc gcgcattgtt gtagacgagc
     2341 ttgtccggcg tgtcccagtt gccctggaag aggttgccga agctgccctt gagaccgatc
     2401 ccgctgttgc gcccgcagaa gccgccggcg tcaacggcgc cggtcaccat cgcatcgaga
     2461 gagctggtgc actggaacca tgcggaaaga ccgcctccca gtttttcctc gcccctgacg
     2521 aacatctcgg gctcggaggt ctcgaggatg tccgtcctct gcccggagct cgaattcttc
     2581 gggctatgcg agtagtagaa cgtggtgatc gagccgccga tctgcaccgt gcttgtctgc
     2641 gccagcacag cggcagacgg cgcggccaga acacccgcga cagcgatcgc cattgctttc
     2701 ttgttcatgc tgaatctcct ttgaagtttt actgccctca gggctatcac atcacgtgtt
     2761 tgcgacaacc ctaatcgatc cccccttacg gcgaggttgg agctagtttg ccggacggca
     2821 taacgggcag gcaacaaaaa cgcgaacttt tcctcgttat ttcaaggaag ttgttgcagg
     2881 tgcgcaacag tttttccggg atgtccatcg catcccggaa aaactgaaaa ttgatatata
     2941 atcaacgcat tatggtctta tttgccgaat accgtcaggc cagactgatt cgcctcaaga
     3001 aagaccccgc cgcatgagca agggagagat agggatacag aacctgatcc aggcgggtct
     3061 gcgcgcgcac cacgccggcc ggctagaaga agcaaaagcg ctttacgaac aggcacttgc
     3121 gatacggcct cggcaccccg atgcattgca cctggccggc gtggttgccc tccaagcggg
     3181 agaagccgag cgcgcggttg cgctgattca aaaagcgatc aaggtgcaac cagacaatcc
     3241 cggatttcac gccaatctcg cgcaggctta tctcgcgtcg cggcgatttg aggacgcgcg
     3301 cgcagccttc cggcgggcgg ccaggctcga gccgcgcgat ccgcagttcg cggtcggcgc
     3361 cgcgaactgt ttagcgatgc agggcagcgt cgcggaagcc gagcgggagt tgcatggcgt
     3421 gacgcagcgc cattacagct acgcactcgc ctggttcaac ctcggtaacg tagtgcagga
     3481 acaggggcgg cagcaggaag cggaggagta ctaccgccgc gccatcgagc tcgacccggc
     3541 gtttgccgat gcacacacca atctcggcgg gg
//
LOCUS       DBNGWZ010000496         1929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_316549, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000496 DBNGWZ010000000
VERSION     DBNGWZ010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_316549"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1483)
                     /locus_tag="AC16CX_15290"
     CDS             complement(38..1483)
                     /locus_tag="AC16CX_15290"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR3013906.1"
                     /translation="MRHPNPAPNRIALLGQKLPAVLFAVFALALAGCVSTGSSPATAV
                     QTPKNIIIMLADGAAPTQWDFGRYASKVLRNQPFVTTDVVFREGVLGLLSTHPSDAYV
                     VDSAAAGSAMSTGHKVNNAAIAVMQDGKPVPTVMEVAKARGKRIGLVTTATVYDATPA
                     AFSLHALSRRDSQGLVDQYLALEPDVLMGGGADFFLPEGAPGGKRKDSRDIIAAFRAK
                     GYHVVRNTAELNAATGAKLLALFADDDMDFELDREPAKEPTTAEMAAAAIKTLSEDSP
                     NGFVLLVENENTDTAGHANDAASLMHALWAFDEAVKVALEFQRRNPDTLVIVTGDHET
                     GGFSPTYALKDLSSSSRSNRFYTGDEHLRMLGRITISLNAANEKLGRKPSAETLDKLL
                     AGHFPGFKLDPDLRERILKRQTLERNFSYLTQNLLGRMVARQTGYYWGTSGHTPEPVV
                     VGAIGPGAELFRGYQDNTDFGKHLHRLIQGK"
     gene            1620..>1929
                     /locus_tag="AC16CX_15295"
     CDS             1620..>1929
                     /locus_tag="AC16CX_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3013907.1"
                     /translation="MHLHILGVCGTFMGGLALIARAAGHRVTGCDANVYPPMSHQLAA
                     QGIELIEGWNADQIALEPDIFVIGNVVTRGNPLMEEILDRGLPYISGPQWLRENILAG
                     K"
BASE COUNT          322 a          588 c          613 g          406 t
ORIGIN      
        1 ctccccatct ctgtggttcg attggggtta ctgttgccta tttgccttgg atcagccggt
       61 gcaggtgctt gccgaagtcg gtgttgtcct ggtagccgcg gaacagctct gccccgggtc
      121 cgatcgcgcc gaccaccacc ggctcgggcg tgtgacccga cgttccccaa tagtagccgg
      181 tctgccgcgc caccatccgg cccagcaggt tttgcgtcag gtagctgaag ttgcgctcga
      241 gcgtctgccg cttcaggatc cgctcgcgca aatcgggatc gagcttgaaa ccgggaaagt
      301 gcccggcgag cagtttgtcg agcgtttcgg cggagggctt cctgccgagc ttctcgttgg
      361 ccgcgttgag ggatatcgtg atgcgcccga gcattcgcaa gtgctcgtcg ccagtataga
      421 accggttgct cctcgacgac gaggacaggt ccttgagtgc gtaggtcggc gagaagccac
      481 cagtctcgtg gtcgccggtg acgatcacca gcgtgtcggg gttgcggcgc tggaactcga
      541 gcgcgacctt gactgcttcg tcgaacgccc atagcgcatg catgagcgat gcggcgtcat
      601 tggcgtgccc cgccgtgtcg gtgttctcgt tttcgaccag caggacgaac ccgttgggac
      661 tgtcctcgga gagcgtcttg attgcggcag cggccatctc ggcggttgtc ggctccttcg
      721 ccggttcgcg gtcgagctcg aaatccatgt cgtcgtcggc gaacagcgcg agcaacttcg
      781 cgccggtggc ggcattgagt tccgcggtat ttctcacgac gtggtaaccc ttggcgcgga
      841 acgccgcgat gatatccctg ctgtcttttc gcttgccccc gggggcccct tcgggcagga
      901 aaaaatccgc gcccccgccc atcagcacgt cgggctcgag cgcgagatac tggtccacca
      961 gtccctgcga gtcacggcgc gacagggcgt gcaggctgaa tgcggccggt gtcgcgtcat
     1021 acacggtggc ggtggtcact agtccgatcc gcttgcccct ggccttcgcc acttccatca
     1081 ccgtaggaac cggcttgccg tcctgcatga cggcaatcgc ggcattgttg accttatggc
     1141 ctgtcgacat cgccgagcct gcggccgcgg aatcgacgac gtatgcatcg ctcggatgag
     1201 tgctgaggag cccaagaacc ccctccctga acacgacatc cgtcgtgaca aagggctggt
     1261 tgcgcagcac tttgctcgcg tatctgccga agtcccactg cgtgggcgcg gctccgtcgg
     1321 ccaacatgat gatgatgttc ttcggcgtct gcaccgcagt tgccggggaa cttccggtgg
     1381 atacgcagcc ggcgagcgcc agtgcgaaga cggcgaacag aactgccggt agcttctggc
     1441 ccagtaaggc aatacgattc ggcgcggggt tagggtgtcg catgatcatt cctcttcgtt
     1501 aagagtggct gcagaaacgc taatggatga taagcgaaac cggaatcgct ggcacgctgt
     1561 gcgctggccc cggtatacaa tgcgcgcttg atttttactg gtgtcttggc ggttcgttta
     1621 tgcatttgca catcctcggc gtctgcggca ccttcatggg cggcctcgcg ctcatcgcgc
     1681 gcgcggcggg gcaccgcgtc accgggtgcg atgccaacgt ctatccgccg atgagccatc
     1741 agctcgccgc ccagggcatc gagttgatcg aaggctggaa cgcggaccag attgcgctcg
     1801 aacccgacat tttcgtgatc gggaacgtcg ttactcgcgg caatccgctg atggaggaaa
     1861 tcctcgaccg gggattgcct tacatttcag gtccgcaatg gctgagggaa aacattcttg
     1921 ccggaaaat
//
LOCUS       DBNGWZ010000497         3011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3337869, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000497 DBNGWZ010000000
VERSION     DBNGWZ010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3337869"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="AC16CX_15300"
     CDS             complement(<1..1918)
                     /locus_tag="AC16CX_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR3013908.1"
                     /translation="MDDQLRKAALDYHRLPTPGKISIAPTKGLINQRDLALAYTPGVA
                     IACQEIAADPAEARNLTARGNLVAVITNGTAVLGLGAIGPLASKPVMEGKGVLFKKFA
                     GIDVFDIEIDERDPARLVEIIASLEPTFGGINLEDIKAPECFIVERKLRARMKIPVFH
                     DDQHGTAIIVGAAVLNGLKVVGKDIRSVKLVVSGAGAAALACLNLLVDMGLPRPNIVV
                     TDIKGVVYKGRKEEMDPEKERFAIETRARKLAEVIDGADIFLGLSAGGVLKPGMASRM
                     AARPLILALANPEPEIRPEEAKKVKPDAVIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTITREMELAAVHAIAELAQAEQSDIVAMAYGGENLKFGPEYLIPRPFDPRLIAKIA
                     PAVARAAMASGVATRPVADFDAYSERLNQFVYQSGLIMKPVFAAAKGSTTRVVYCEGE
                     DERMLRAAQVVVDEGIARLTLVGRREVVRMRIERLGLRIRMDADFDLVDPNNDPRYRE
                     YSMAYHKLAERKGVTPQIARLEMRRRYTLIGAMLMHMRAADTMLCGVVGRYESHLRFV
                     DHVIGLRPGVKNYAAMNTLLLPNRTVFICDTYVNYDPTAEQIAEMTVLAAEEIRRFGI
                     TPKVALLSHSSFGTA"
     gene            2163..>3011
                     /gene="pip"
                     /locus_tag="AC16CX_15305"
     CDS             2163..>3011
                     /gene="pip"
                     /locus_tag="AC16CX_15305"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HIR3013909.1"
                     /translation="MADISHTPRAELYPEIEPYSAGTLKLDGVHTLYWEQSGNPEGAP
                     VLFLHGGPGAGASPAHRRFFDPAHYRIVIFDQRGAGRSTPLGELRDNTTPRLIADIER
                     LRSHLGIERWLVFGGSWGSTLALAYGEAHPERCTGFILRGVFLCRRSEIEWFLYGLRN
                     LFPEAWRSFAEVMPASERGDLLAAYYKRLTDPDPGVHLPAARAWSTYEGVCSTLLPSP
                     ETVAYFAGDGVSLGLARIEAHYFSHDIFLPENSLLANVGRIRHLPCVIVQGRYDAVCP
                     IATADEL"
BASE COUNT          456 a         1018 c         1009 g          528 t
ORIGIN      
        1 tggcggtccc gaagctcgag tgcgacaaca gcgcgacttt gggcgtgatg ccgaagcgcc
       61 ggatctcctc cgccgcgagc accgtcatct ccgcgatctg ctccgcggtg gggtcgtagt
      121 tgacgtaggt atcgcagatg aacacggtgc ggttgggcag cagcagcgtg ttcatcgcgg
      181 cgtagttctt cacgcccggc cgcagcccga tgacgtggtc cacgaagcgc agatggctct
      241 cgtagcgtcc gaccacgccg cacagcatgg tatccgccgc ccgcatgtgc atgagcatgg
      301 cgccgatcaa ggtgtagcgc cggcgcatct cgaggcgggc gatctggggc gtgacgccct
      361 tgcgctcggc aagcttgtgg tacgccatcg agtactcgcg atagcgcgga tcgttgttgg
      421 ggtccaccag atcgaagtcc gcgtccatcc ggatcctcag accgagccgc tcgatgcgca
      481 tccggacgac ttcccggcgg ccgaccagcg tcaggcgcgc gatgccttcg tccaccacca
      541 cctgcgccgc ccgcagcatc cgctcgtcct cgccctcgca atacaccacg cgcgtggtcg
      601 agcccttggc cgccgcgaac accggcttca tgatgaggcc ggattggtag acgaactggt
      661 tgaggcgctc gctgtaggcg tcgaaatcgg cgacggggcg cgtcgcaacg ccgctcgcca
      721 tcgcggcgcg cgccaccgcc ggcgcgatct tggcgatcag gcgcggatcg aacggccgcg
      781 ggatgaggta ttcaggcccg aacttgaggt tctcgccgcc gtaggccatg gcgacgatgt
      841 ccgactgctc ggcctgcgcc agttctgcga tcgcatgcac cgcggcgagc tccatctcgc
      901 gggtgatggt ggtcgcgccc acgtcgagcg cgccgcggaa gatgaacgga aagcacagga
      961 cgttgttgac ctggttcggg tagtcggagc gtccggtcgc gatcaccgcg tcgggcttga
     1021 cctttttcgc ctcctccggc cggatctccg gctcggggtt ggcgagcgcg aggatgaggg
     1081 gccgcgcggc catgcggctc gccatcccgg gcttgagcac ccctccagcg gagaggccca
     1141 ggaagatgtc ggcgccatcg atcacttcgg cgagcttccg tgccctggtc tcgatcgcga
     1201 agcgctcctt ctcgggatcc atttcctcct tgcgcccctt gtagaccacg cccttgatgt
     1261 cggtcacgac gatgttcgga cgcggcagcc ccatgtcaac gaggaggttc aggcaggcga
     1321 gcgccgccgc gccggcgccg gagacgacga gcttcacgct ccggatgtcc ttgccgacca
     1381 ccttgaggcc gttcaggacc gcggcgccga cgatgatagc ggtgccgtgc tggtcgtcgt
     1441 ggaataccgg tatcttcatg cgggcgcgga gcttgcgttc cacgatgaag cactcgggcg
     1501 ccttgatgtc ctcgagattg atgccgccga aggtgggctc gaggctggcg atgatctcga
     1561 ccaggcgggc ggggtcgcgc tcgtcgatct cgatgtcgaa gacgtcgatg ccggcgaact
     1621 tcttgaacag cacgcccttg ccctccatga cgggcttgga ggcgagcggc ccgattgcgc
     1681 ccagtcccag caccgcggtg ccgttggtga tcaccgccac gagattgccg cgcgcggtca
     1741 gatttcgcgc ttcggcgggg tcggcggcga tttcctggca ggcgatcgcc acgcccggcg
     1801 tataggcaag cgcaaggtcg cgctgattga tgaggccctt ggtgggagcg atcgagatct
     1861 tgcccggggt cggcaaccga tggtaatcga gcgcggcctt gcgcagttga tcgtccattg
     1921 ggcccgtcta gaaggcggcg cgcgttgaac aacgcggagc gcccgttcga acgtccgcag
     1981 ttaaaccgga gcacagcgtc cggccttatc gcgcgttgag cggcacatcg tgtccgttcg
     2041 aacgcgcgga gttaaaccgg cgcgcagcgt ccggttttaa cggaaattat accggatgtg
     2101 ccggcgcctg atttctagac cgccccggct atgctatgtt ttccgcagca gcgctaccgc
     2161 ttatggccga catttcccac acgccgcgcg cggaactcta tcccgagatc gagccctaca
     2221 gcgcgggcac gctgaagctg gacggcgtgc acaccctgta ctgggagcag tcgggcaatc
     2281 cggaaggcgc accggtgctg ttcctccacg gcggccccgg cgcgggcgcc tcccccgccc
     2341 accggcgctt cttcgacccg gcccactacc ggatcgtcat tttcgaccag cgcggagccg
     2401 gccgctccac gccgctcggg gagctgcgcg acaacacgac cccgcgcctg atcgccgaca
     2461 tcgagcggct gcgcagccac ctgggaatcg agcgctggct cgtgttcggc ggctcgtggg
     2521 gcagcacgct cgcgctcgcc tacggcgagg cgcacccgga gcgctgcacg gggttcatcc
     2581 tgcgcggcgt gttcctgtgc cggcgcagcg agatcgagtg gtttctctac ggcctgcgca
     2641 acctgttccc ggaggcctgg aggtccttcg ccgaagtgat gcccgcttcc gagcgcggcg
     2701 atctcctggc cgcctactac aagcgcctga ccgatcccga cccgggcgtg cacctgccgg
     2761 ccgcgcgcgc gtggagcacc tacgaggggg tctgctcgac gctgttgccg agcccggaaa
     2821 cggtcgccta cttcgcggga gacggggtgt cactcgggct cgcgcgcatc gaggcgcatt
     2881 acttcagcca cgacatcttc ctgcccgaga attcgctgct ggcgaatgtc ggccgcatcc
     2941 gccacctccc ctgcgtgatc gtgcaggggc gttacgacgc ggtgtgtccg atcgccaccg
     3001 cggatgagct c
//
LOCUS       DBNGWZ010000498         3606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_570566, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000498 DBNGWZ010000000
VERSION     DBNGWZ010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_570566"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1020
                     /locus_tag="AC16CX_15310"
     CDS             235..1020
                     /locus_tag="AC16CX_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3013910.1"
                     /translation="MSDTVLYSVADAVATITLNRPQVMNALDAEMIRQLREATERVER
                     DSAVRAVVMRGEGAAFLAGGDVAMFHANLPRMPALVREGAAEFHHAILALRRAPKPVL
                     ASVHGAVAGAGVSLMAAADLAIAADDAKFTLAYSGIGTSPDGGATWSLPRLVGYRKAL
                     ELMMLSDTFDARTAFELGLVNWVVGAERLTVETGSIARRLAQGATLAFGEIKRLVYQS
                     QDQTLAAQLNAEVEAFARCAGTRDFAEGVTAFVEKRKPLFKGE"
     gene            1041..1859
                     /locus_tag="AC16CX_15315"
     CDS             1041..1859
                     /locus_tag="AC16CX_15315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3013911.1"
                     /translation="MGLKGKTIFITGATRGIGREIALKCARDGANVVVTGKTAEPHPR
                     LSGTIHTVAKEIESAGGAALAIQLDVRDAEAVAVAVRRTAEVFGGLDCLVNNASAISL
                     TGTLETPVRRLDLMLAVNLRGTFVCSQACIPHLKKAANPHILNLSPPLNMHPRWFKDH
                     VAYTMAKYGMSMCTLGMAEELRAHGIAVNSLWPRTTIATAAIEVNFPEAMLRASRKPA
                     IMAEAAYAILNRDSRRTTGNFYIDETVLREEGVTDFDQYAVTPGTSLYTDLFLD"
     gene            2006..>3606
                     /locus_tag="AC16CX_15320"
     CDS             2006..>3606
                     /locus_tag="AC16CX_15320"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR3013912.1"
                     /translation="MSQASHLIPFETAATLDGLFRERVKRTPDLVAYRAHNEQHNNWR
                     DYTWAQINHQVARWQVALEKDGLKAGDRVAVMLKNSPEWVTYDQAALGLGLVVVPLYT
                     QDRPENVAYIISDSGSKVLLIEGAEQWQALSEVRGQLTGLTRILSVNPVPNAGEPRLK
                     PLAEWLPESGGETRHVPRDPHALATIVYTSGTTGRPKGVMLSHNNILSNAAASCDVLT
                     AGHEDLFLSFLPLSHTFERTCGYYLTIMTGSTTAYSRGIPQLAEDLQAIRPTLLISVP
                     RIYERVYGRIRAALDEGPPLKKKLFLLAVEVGYARFEHEQGRGSWKPSFLLWPLLNAL
                     VAKKILARLGGRMRAAISGGAALPPEISRVFLGMGLTVLQGFGMTESSPVACANRPDD
                     NLPASVGKTLPGVQVRIGENNALMIKGPNVMLGYWNNPEATKAILGADGWLNSGDTAR
                     IDSQGHVFITGRLKEIIVLSNGEKVPPVDIEAAIARDPLFEQVMLLGEGKPYLSVMTV
                     LNADHWKKLCGERGLDAGTPRGKPV"
BASE COUNT          689 a         1140 c         1190 g          587 t
ORIGIN      
        1 gacgattttc cgaaggacct cggcaagacc gaaatccacc agaccacgca gccggtcacg
       61 ttcgaggcgc tgcgcggcag gacctcgtgg ccggagatgg aacagcgcgg ccaccagccg
      121 aatccgggcg cctgataggg cagccgtcag cagtcagcga tcagctgtca gcttaaacgg
      181 ggtccacctt ttttgctgac tgctgaacac tgatagctgg atactgagta ttgtatgagc
      241 gacaccgtcc tgtattcagt ggccgacgcg gtggcgacga tcacgctcaa ccgtccgcag
      301 gtgatgaatg cgctcgatgc ggaaatgatc cgccagctgc gcgaggccac tgaacgagtt
      361 gagcgcgact ccgcagtacg cgcggttgtc atgcggggcg agggagcggc gtttctcgcc
      421 ggcggggatg tcgcaatgtt tcacgccaat ctaccgcgca tgccagctct ggtaagggaa
      481 ggcgcggccg aatttcacca cgcgatcctg gcgctgcggc gcgcgcccaa gccggtgctc
      541 gccagcgtgc acggcgccgt cgccggcgcg ggagtgagcc tgatggcggc cgccgacctc
      601 gccatcgccg ccgatgacgc gaaattcacg ctggcttaca gcgggattgg cacgagcccc
      661 gacggcggcg cgacctggtc cctgccgcgc ctggtgggct atcgcaaggc gctggagttg
      721 atgatgctgt cggacacctt cgacgcgcga acggcgttcg agctggggct cgtcaactgg
      781 gtggtgggcg cggagcggct gacggtcgaa accgggagca tcgcgcgccg gctggcgcag
      841 ggcgcaacgt tggcattcgg cgagatcaag cgtctcgtct accagagcca ggatcagacg
      901 ctcgcagccc agctcaacgc cgaagtggag gcgtttgcgc gctgcgccgg cacgcgcgac
      961 ttcgccgaag gcgtcaccgc attcgttgaa aagcgcaagc cgcttttcaa gggagagtga
     1021 tgagtgatgg gtgatgggtg atggggctca agggcaaaac gatattcatc acgggggcga
     1081 cgcgcggcat cggtcgcgag atcgcgctca agtgcgcgcg cgacggcgcc aacgtcgtgg
     1141 taaccggcaa gaccgctgag ccgcaccctc ggctctccgg caccatccat accgttgcaa
     1201 aggaaatcga gtccgccggc ggagcggcgc tggcgattca gctcgatgtg agagatgccg
     1261 aagcagtggc ggttgcggtg aggcgcacag cggaggtctt tggcggcctc gattgcctgg
     1321 tcaacaatgc aagcgcgatc tcgctcaccg gcacgctgga gacgcccgtc aggcgcctgg
     1381 atctcatgct ggcggtcaat ctgcgcggca ccttcgtctg ctcgcaggcc tgcattcccc
     1441 atctcaaaaa ggcggccaac ccgcacatac tcaacctctc gccgccgctc aacatgcacc
     1501 cgcgctggtt caaggaccat gtcgcctata ccatggcaaa atatggcatg agtatgtgca
     1561 cgctcgggat ggcggaggag ctgcgtgcgc acggcatcgc cgtgaactcg ctgtggccgc
     1621 gcacgaccat cgcaaccgcc gcgatcgagg tgaacttccc ggaagcgatg ctcagggcca
     1681 gccgcaagcc cgcgatcatg gccgaggcgg cgtatgcgat actcaaccgt gacagccgcc
     1741 gcacaacagg caatttttac atcgacgaga cggtgctgcg ggaggagggc gtcacggact
     1801 tcgatcagta tgccgtcact ccaggaacaa gtctgtatac cgacttattc ctggactgat
     1861 cgaagtcaga ggatgaagga ggaaggcgga aggatgacct ggttcagact cccgcactgc
     1921 gggggtcatc tctcatccct cattcttcat ccttttgatt ccgatcactc atgacatagc
     1981 gacacaccag taaaaaggag gagcgatgtc acaagccagc cacctgatcc cgttcgaaac
     2041 cgcggcgacg ctcgacggcc tgttccgcga gcgcgtcaag cgcacgcctg acctcgtcgc
     2101 ctaccgcgcc cacaacgagc agcacaacaa ctggcgcgat tacacctggg cgcagatcaa
     2161 ccaccaggtc gcgcgctggc aggtcgcgct ggaaaaagac ggcctcaagg ccggcgatcg
     2221 cgtcgcggtg atgctgaaga actcgcccga gtgggtcacc tacgatcagg cggcgctcgg
     2281 cctgggactg gtggtggtgc cgctctatac gcaggaccgg ccggaaaacg tcgcctacat
     2341 catcagcgat tcgggttcca aggtgctgtt gatcgagggc gccgagcaat ggcaggcgct
     2401 gtccgaggtc aggggccagc tcaccgggct gacacgcatc ctgtcggtga acccggtgcc
     2461 caacgcgggc gagccgcggc tgaagccgct ggcggaatgg ctgccggaga gcggcggcga
     2521 gacacgccac gtgccacgcg atccgcacgc gcttgccacc atcgtctaca cctcgggcac
     2581 gaccggccgg cccaagggcg tgatgctctc gcacaacaac atcctttcca acgcggccgc
     2641 gagctgcgac gtgctgacgg cgggtcatga ggacctgttc ctgtccttcc tgccgctctc
     2701 gcacaccttc gagcgcacct gcggctacta cctcaccatc atgaccggct ccacgaccgc
     2761 ctactcccgc gggatccccc agctcgcgga ggacttgcag gcgatacgcc cgacgctgct
     2821 gatctccgtg ccgcggatat acgagcgcgt ttacggccgg atccgcgccg cgctcgacga
     2881 aggcccgccg ctgaagaaga aactgttcct gctggcggtg gaagtgggtt acgcgcgctt
     2941 cgagcacgag cagggccgcg gctcgtggaa gccatcgttc ctgctatggc cgctattgaa
     3001 cgcgctggtg gcgaagaaga tcctggcgcg actgggcgga cggatgcgcg ccgccatttc
     3061 cggcggcgcc gcgctgccgc ccgagatctc tcgggtgttc ctcggcatgg gactcacggt
     3121 gctgcagggt ttcggcatga cggaatcgag ccccgtcgcc tgcgccaacc gtccggacga
     3181 caacctgccg gcaagcgtgg gcaagacgct gcccggcgtg caggtgagga tcggggaaaa
     3241 caacgcgctc atgatcaagg ggccgaacgt catgctcggt tactggaata atccggaagc
     3301 gaccaaggcc atccttggcg cggatgggtg gctcaactca ggggatacgg cgcgcatcga
     3361 ctcgcagggc cacgtcttca tcacggggcg actgaaggag atcatcgtgc tctccaacgg
     3421 cgagaaggtg ccgccggtgg acatcgaagc cgcgatcgcg cgcgatcccc tgttcgagca
     3481 ggtgatgctg ctcggggagg gcaagccgta cctgagcgtg atgacggtat tgaatgccga
     3541 tcactggaaa aagctctgcg gggagcgcgg cctcgatgcg ggaacgccgc gcggcaagcc
     3601 ggtcga
//
LOCUS       DBNGWZ010000499         6209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1451356, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000499 DBNGWZ010000000
VERSION     DBNGWZ010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1451356"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1079
                     /locus_tag="AC16CX_15325"
     CDS             174..1079
                     /locus_tag="AC16CX_15325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3013913.1"
                     /translation="MSSQPAVGAEAPTCAPPDLNPRKPKLEFPPRACDTHAHIMGPKA
                     RYAYSPARVYTPPDCLLPDYLRMLDTLGVERAVLVQPSVYGTDNTVMLEAMKAAGKRF
                     RGVAVVADDVSDAELKKLDATGVRGVRVNIVDVKDRKPGTLPMDMLKDLARRVAPLGW
                     HMEFLMHADEFPDLDRAFADFPVEIVLGHLGYMKTDKGLDNAGFQALLRLMKAGRAWV
                     KLTGPSRITTIGGLPYADVAPYAHVLLKAAPQQVIWGTDWPHVIIKTPMPNDGDLADL
                     LSDWIPDAALREQVLVHNPTRLYGF"
     gene            complement(1098..3152)
                     /locus_tag="AC16CX_15330"
     CDS             complement(1098..3152)
                     /locus_tag="AC16CX_15330"
                     /inference="COORDINATES: protein motif:HMM:NF014474.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR3013914.1"
                     /translation="MNPPVKTTAIPLRGPLFRGGRRLRIALVGLPGCGKSTLFRAVAA
                     TSVSTGALAGTRRAYDECAVQIGLDEARVIDLPSVRSLRDPEPADRASLKYLLWGDER
                     PPVAVHEPQGPPVPLAAPDVIVQVIDATALERHLELTLELLQLGRPLVIALNKMDEAR
                     EKGLHVGTKVLARHFGVPVVPTIAVMGHGIKQLFAATVEAVRQGVCLLPPPPGPHLHD
                     ALRRISEPLRAREVLAAFRVPLHFLAARVGEGDRYFLDELRQHFPKQSAEVHRLLAGA
                     DRSLPRPLRDELHADRHYRAAIAAEAATRPGGVRERRNWRYWLDELFLSRRWGLAGSA
                     AVFAAVLFVVFDVSAWLDSMTSARLVEWASAWQPQTTAGVIERAVADGLIGLVGIVVP
                     YMIPLVLLLVALEQVGIMARIAFAVDRGFHRLGLHGDVAVPFLLGLGCNVPAISEIGA
                     RSRGSERVVASLLVMFVPCSARSAIILALAGKYLGAGGVLAIFLLAMLVIAVLGQVLR
                     RRYPTSGPGQVQEIPPYAMPRWRPLLAETWLRTRDILTIVMPLLVGGSIVLALLSHVG
                     ADRVLNALLTPVTQWWLGLPVVLGVPLLFGVLRKELSLLMIFQALGTFEIDHYLGAVQ
                     IFTFLLFLTFYLPCISTFAVMLKTIGRREAMFAVLLSIIVALMVSGVARFTLEAFGLL
                     AA"
     gene            complement(3359..4261)
                     /locus_tag="AC16CX_15335"
     CDS             complement(3359..4261)
                     /locus_tag="AC16CX_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR3013915.1"
                     /translation="MAASQEKTKVVLGTATPGGGFPAYGWPYAEVLNETDPTLAIEPI
                     NTKGSTENAPRLERGDLDLGLVTGEVTYEAISGIGGPKARNLRIINATYSQAGMFMVT
                     AGSPYRSISDLKGKPVVWGAGTSGFIVLARYVMDGLGLDMKKDFQAILLEKAGDGPPL
                     VLDGRAAAMWGGGVGWPPFMAIAKGPAGARFIAPSADEIKRITTKHSFLKPNVLPAGS
                     YPGQDKPITSVGSWPFVLARASLPDDVAYRLARALHKGHAVLSKKLDQAKESTLENTI
                     AAAPCRDLIHPGVLKYMREIGLLK"
     gene            complement(4331..5338)
                     /locus_tag="AC16CX_15340"
     CDS             complement(4331..5338)
                     /locus_tag="AC16CX_15340"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013916.1"
                     /translation="MKRVGRFAGQLAAVGGAIALALAALSAGAQSAATQAPEFPSKPI
                     RFLVGVAPGGGTDFVARLIGQKLADKWGQPVVTDNRTGATGLIALGMTANATPDGYTC
                     IVFNIGHLMSAALSRKAGFDTVKDFAPVSLIATGTLLLAVHASVPGGSLVEFIAYAKS
                     QPGKLNYASGGPAGVQHLAMELLKREAKIDLVHVPYKGSGPGTIALLSGQVQAFITNI
                     LALHPHIRAGKLKALAVANPKRNPLAADVPTFAELGYPSVDVNLWQGIMVPARTPAPV
                     VEKLANAITEAVRAPDSAARLATQGAEPAGSTPREFAAFLRNERTRWLALAKEANITV
                     D"
     gene            complement(5388..>6209)
                     /locus_tag="AC16CX_15345"
     CDS             complement(5388..>6209)
                     /locus_tag="AC16CX_15345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3013917.1"
                     /translation="VYDIRPEAVKAFLSQHGGKAAASLVDAAHGADAVITMLPDDKVV
                     RKVVLGEGSENYVAAGLAKGAVVIDMSTCNPAATRSLAEALKPRGIEVVDAPVMGGVI
                     FAKDATLDIMVGGDTELIELVKPVLQAMGRNIVSCGGTGAAHAMKALANYVNACALIN
                     VIEAMTIGRRFGLDAKLMAEALIPMCAGRNHPIEKKVIPHVLTRKYGTGMAMGFIAKD
                     VRIAMETAKSVGAFAPLGERVSELWTAAAEKLGANLDQTQIARYWEEASGARLDG"
BASE COUNT         1075 a         2152 c         1969 g         1013 t
ORIGIN      
        1 aatccatgtg ccgccttctg agggaactgt tccgcagcag tcgtgccgga tcgaatccag
       61 cctccgtgct gctttgggcg accgccctca ttcgcatgcg gccacacgtg acctgcaccg
      121 gtcattcatt caacagacaa acgtgatcca tccccagatt gcgagggcga tcaatgagct
      181 cgcaacctgc ggtcggcgcc gaggcgccga cctgcgcgcc gccggacctc aatccgcgca
      241 agcccaagct cgagttcccg ccgcgcgcgt gcgacaccca cgcccacatc atggggccga
      301 aggcgcgcta cgcctattcg ccggcgcgcg tctacacccc gcccgactgc ctgctgcctg
      361 attacttgcg catgctcgac acgctcggcg tcgagcgcgc ggtgctggtg caacccagcg
      421 tctacggcac cgacaacacg gtgatgctgg aagcgatgaa agcggccggc aagcgttttc
      481 ggggggtcgc agtcgtcgcc gatgacgttt cggatgcgga actgaagaag ctcgacgcca
      541 ccggcgtgcg tggcgtgcgc gtcaatatcg tggacgtgaa ggaccgcaag ccgggcacgc
      601 tgccgatgga catgttgaaa gacctcgcac gccgcgtcgc gccgctcggt tggcacatgg
      661 aatttctcat gcacgcggac gagttcccgg atctcgaccg cgcgttcgcc gattttccgg
      721 tcgagatcgt gctcgggcat ctcggctaca tgaaaaccga caaggggctc gacaacgccg
      781 ggtttcaggc gctgctgcgc ctgatgaagg cgggccgcgc ctgggtgaag ctgaccggcc
      841 cgtcgcgcat caccacgatc ggcgggctgc cgtacgccga cgtcgctccc tacgcgcacg
      901 tactgctaaa ggccgcgccg caacaggtca tctggggcac cgactggccc cacgtgatca
      961 tcaagacgcc gatgccgaac gacggcgacc tcgccgacct gctttccgac tggattcccg
     1021 acgccgccct gcgcgagcag gtgctagtgc acaatccgac acggctttac ggcttttagc
     1081 cggattgcgc gtgtatttca ggccgccaat agtccgaacg cttcgagtgt gaagcgcgca
     1141 accccgctta ccatcagcgc gacaattatc gacagtaaca ctgcaaacat cgcctcgcga
     1201 cgccctatcg tcttcagcat caccgcgaag gtcgaaatgc agggcagata gaacgtgaga
     1261 aacagcagga acgtgaagat ctggaccgca cccaggtagt gatcgatctc gaaggtgccg
     1321 agcgcctgga agatcatcag cagcgacagc tccttgcgca ggaccccgaa caggaggggc
     1381 acgcccaaga cgaccggtaa tcccagccac cactgcgtga cgggcgtgag cagcgcgttg
     1441 agcacgcggt ccgccccgac gtggctcaag agcgccagca cgatgctacc gcccaccagg
     1501 agaggcatga cgattgtaag aatgtcccgc gtgcgcagcc aggtttccgc gagcagcggg
     1561 cgccagcgcg gcatcgcata aggcgggatc tcctgcacct gccccggacc gctcgtggga
     1621 tagcgccgtc gcagcacctg ccccagaaca gcgatgacga gcatcgcgag caggaagatc
     1681 gcgagcaccc ctcccgcgcc gaggtatttt ccggcgagcg caagaatgat cgcagagcgc
     1741 gccgaacagg gcacgaacat caccagcagc gacgccacga cacgctcgct gccacggctg
     1801 cgagcgccga tctccgagat tgcagggacg ttgcatccca gcccaaggag gaacggcact
     1861 gctacgtcgc cgtgcagccc cagccggtga aagccccggt ccaccgcgaa cgcaatgcgc
     1921 gccatgatgc ccacttgctc cagcgcgacc agcagcagca caagcgggat catgtatggc
     1981 acgacgatgc ccacgagccc gatcaggccg tcggccaccg cgcgctcgat cacgcccgcc
     2041 gtcgtttgcg gctgccacgc ggacgcccac tccacgagcc gggccgaggt catcgagtcg
     2101 agccaagcgc tgacgtcgaa caccacgaac agcacggcag caaacacggc tgcgctcccg
     2161 gcaagtcccc accgccggct caggaacagc tcgtcgagcc agtaacgcca attgcggcgc
     2221 tcgcgcactc cgcccggccg cgtcgccgcc tccgccgcga tcgcggcgcg gtagtggcgg
     2281 tcggcatgca gctcatcgcg cagcgggcgc ggcagcgagc ggtccgcccc ggcgagcaga
     2341 cgatgtacct cggccgactg cttcgggaaa tgctgcctca gctcatcgag gaagtagcgg
     2401 tcgccttctc caacccgcgc ggcgagaaag tggagcggga cacggaatgc ggcgaggact
     2461 tcccgcgcgc gcaagggctc cgagatccgg cgcagggcgt catgaaggtg cggtcccggc
     2521 ggcggtggca gcaggcacac accctgccgc acggcctcga ccgtcgcggc gaacagctgc
     2581 ttgatgccgt gtcccattac cgcgatcgtg ggcacgaccg gcacgccgaa gtggcgggcc
     2641 agcaccttgg tgccgacgtg cagccccttt tcgcgcgcct cgtccatctt gttgagcgcg
     2701 atgacgaggg gccgccccag ctgcaacagt tccagggtaa gctcaaggtg ccgctcgagc
     2761 gcggtggcat cgatcacctg cacgatgaca tccggggccg cgagcggcac cggcggcccc
     2821 tggggttcgt gtaccgcgac gggtggccgt tcgtcgcccc acaggagata cttgagcgag
     2881 gcccggtccg cgggctccgg gtcgcgcagc gagcgcacgc tcggcagatc gatcacgcgc
     2941 gcctcgtcga gcccgatctg caccgcgcac tcgtcgtacg cgcgccgcgt acccgccaga
     3001 gcgccggtgc tgaccgacgt cgctgcgacc gcgcggaaga gcgtgctctt gccgcaaccg
     3061 ggcagcccga cgagcgcgat ccgcaaccgg cgcccgcccc ggaagagcgg gccgcgcagc
     3121 gggatggcag tggtcttgac gggaggattc atggcgtcgt gagcccgtct gcacaggctc
     3181 acgccatgct agcgcccgcc actacgcgca aatgtgatcc agatcaaatc ttcacggcgc
     3241 aatccggacc gtttcgacgg ccttgggccg gattgcgcgt gacttgcggg tcgcggctcg
     3301 tggcttgacc tatccgcgat ccgccatccg cgacctgcga gccatgattc gcgagccact
     3361 atttgagaag gccgatctcc ctcatgtact tgagcacgcc gggatgaatc aggtcccgac
     3421 acggtgcggc ggcgatcgtg ttttccagcg ttgattcctt cgcctgatcg agcttcttgc
     3481 tcagcactgc gtgccccttg tgcaaagcgc gagcgagcct gtaggcgacg tcgtccggca
     3541 gcgaggcgcg cgcgagcacg aacggccacg agccgaccga cgtgattggc ttatcctggc
     3601 cgggataact gccagcggga agcacgttgg gtttgagaaa cgaatgcttc gtcgtaatgc
     3661 gcttgatttc atccgcgctc ggcgcgatga agcgcgcgcc cgccggcccc tttgcgatcg
     3721 ccatgaacgg cggccagccg acgccgccgc cccacatcgc tgcggcgcgc ccgtcgagca
     3781 ccaagggtgg gccgtctccc gccttctcca gcaggatcgc ctggaaatcc ttcttcatgt
     3841 cgagaccgag gccgtccatc acatagcgcg cgagcacgat gaagccggaa gtgcccgccc
     3901 cccacaccac gggcttgccc ttgaggtcgg aaatgctgcg ataagggctg ccggccgtca
     3961 ccatgaacat gccggcctgc gaataggtgg cgttgataat gcgcaggttc ctcgccttgg
     4021 gcccgccgat gccggaaata gcttcgtaag tgacctcgcc ggtgacgagc ccgaggtcga
     4081 gatcaccgcg ttccagccgc ggcgcgttct cggtgctgcc cttggtgttg atcggctcga
     4141 tcgcgagcgt cgggtccgtc tcgttcagga cctccgcgta cggccagccg taggcgggaa
     4201 agcctccgcc cggcgtcgcc gttccgagca ccaccttcgt tttttcttgt gacgcagcca
     4261 taccgattcc gcccctccgc aatgtgagat tgctttcaag ccaagccggg ggtccgtgcc
     4321 ggcgcgcgct tcaatcgacg gtgatattcg cctctttggc gagcgcgagc cagcgagtgc
     4381 gctcgttcct gaggaacgcc gcgaattcgc gcggcgtgga ccccgccggt tcggcgccct
     4441 gcgtggcgag ccttgccgcc gagtcgggcg cgcgcaccgc ctcggtgatg gcgttcgcga
     4501 gcttctccac gaccggcgcc ggcgtgcgcg cgggcaccat gatcccctgc cacaggttca
     4561 cgtccaccga aggatagccg agctccgcaa acgtgggcac atcggccgcg agaggattgc
     4621 gtttcgggtt cgcgaccgcc agcgccttga gttttcccgc cctgatgtgc gggtgcagcg
     4681 cgaggatgtt ggtgatgaag gcctgcacct gtcccgacag cagcgcgatg gtgccggggc
     4741 cgctgccctt gtagggcacg tgcacgagat cgatcttcgc ctcgcgcttg agcagctcca
     4801 tggcgaggtg ctgcacgcct gcgggcccgc ccgaggcgta gttgagcttg cccggctgcg
     4861 atttcgcgta agcgatgaat tcgaccaagc tgccgccggg caccgatgcg tgcaccgcca
     4921 gcagcagcgt gcccgtcgca atcagcgaca ccggcgcaaa gtccttcacc gtatcgaacc
     4981 ccgccttgcg cgacagcgcg gccgacatga ggtggccgat gttgaacacg atgcaggtgt
     5041 agccgtcggg cgtcgcattg gccgtcattc ccagggcgat caggccggtc gcaccggtgc
     5101 gattgtcggt cacgacgggt tgaccccatt tgtcggccag cttctggccg attaaccgcg
     5161 cgacgaaatc ggttccgccg cccggcgcca cgccaaccaa gaaacggatc ggcttcgatg
     5221 gaaactccgg agcttgtgtt gcggcggact gcgcaccggc cgacaacgcc gcaagagcca
     5281 gcgcgatcgc ccctcccacg gcagccagtt gtccggcaaa acggccaacg cgcttcatgc
     5341 gcggccctcc cctcggaatg attatagtcg ggctgcgtgg aacggcacta gccgtcgaga
     5401 cgtgcgccgc tcgcttcttc ccagtagcgc gcgatttgcg tctggtcgag gttggcgccg
     5461 agcttctccg ccgccgccgt ccacagttcc gagacgcgct cgccgagtgg tgcgaaggcg
     5521 ccgacggatt tcgcggtttc cattgcgatt ctcacgtcct tagcaatgaa gcccatcgcc
     5581 atgccggtgc catatttgcg ggtgagcacg tgcggaatca ccttcttctc gatcggatgg
     5641 ttgcgcccgg cgcacatcgg gatcagcgct tccgccatca gtttcgcgtc cagcccgaag
     5701 cgcctgccga tcgtcatcgc ctcgatgacg ttgatcagcg cgcaggcgtt cacgtaattg
     5761 gcgagcgcct tcatcgcgtg cgcggcgccc gtcccgccgc aggagacgat gttgcggccc
     5821 atcgcctgca agaccggttt caccagctcg atcagctccg tgtcgccgcc caccatgatg
     5881 tcgagcgtcg cgtctttcgc gaagatcacc ccgcccatca ccggcgcgtc caccacctcg
     5941 atgccgcgcg gcttgagcgc ctcggccagc gagcgcgtgg cggctggatt gcacgtgctc
     6001 atgtctatga cgaccgcacc tttcgcgaga ccggcggcga cgtagttttc cgaaccctcg
     6061 cccagcacca ccttgcgcac caccttgtca tcgggaagca tggtgatcac agcgtccgcg
     6121 ccgtgcgcgg cgtccaccag cgaggccgcc gccttcccgc cgtgctgcga aaggaaagct
     6181 ttcaccgcct ccgggcggat gtcgtaaac
//
LOCUS       DBNGWZ010000500         3833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_317296, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000500 DBNGWZ010000000
VERSION     DBNGWZ010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_317296"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="AC16CX_15350"
     CDS             complement(<1..843)
                     /locus_tag="AC16CX_15350"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3013918.1"
                     /translation="MPIANVRGVSIHYEVLGTKGPWVTLSPGGRRAIEGVESLARRIA
                     GAGYRVLVHDRRNCGASDVVIEGNESENEIWADDLHELLRRQNALPAWVGGSSSGCRL
                     ALLFALRHPQAVRGLLLWRVTGGLAAAKRLAHRYYGQYLPIAQQGGMAAVCATEEFSE
                     RIRERPENRERLMKMDPQRFIEVMSNWNRKFLDDAGKPVIGATEAELKTIRAPACIVP
                     GNDLSHLRSAAENLGRLMRDAEVHPLITKHYDLDVAPREEWDAREAELAAIFLGFMAR
                     VDSRV"
     gene            complement(918..1307)
                     /locus_tag="AC16CX_15355"
     CDS             complement(918..1307)
                     /locus_tag="AC16CX_15355"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR3013919.1"
                     /translation="MEIRYEFGFDAAHRFPDMPRGHRYRGVHGHSFRVEVALRGAPRP
                     PHGFVADFARLERACSGLHRRLDHALLNEIKGLKKPSLENLCLWMWERLSGKFPGLSR
                     VTVRRDSLGQSCTYLGPAGRKIVDRGS"
     gene            complement(1346..1978)
                     /gene="queE"
                     /locus_tag="AC16CX_15360"
     CDS             complement(1346..1978)
                     /gene="queE"
                     /locus_tag="AC16CX_15360"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HIR3013920.1"
                     /translation="MYSVKEIFYTLQGEGANTGMPAVFLRFAGCNLWSGREKDRAKAI
                     CRFCDTDFAGTDGEDGGKFRSAGALANAVEAKWPKRAAAPRFVVCTGGEPLLQLDAPL
                     VKALHARGFRVAIETNGTLEPPPGIDWICVSPKARAPLRLTRGDELKLVYPQRGAGPD
                     RYEGLDFDHFFLQPMDGPYRVRNTRLAAGYCLRHPRWRLSLQVHKLIGIP"
     gene            complement(2095..2388)
                     /locus_tag="AC16CX_15365"
     CDS             complement(2095..2388)
                     /locus_tag="AC16CX_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013921.1"
                     /translation="MRQIASALLIATVLACAVAPAASAELQVGSGDTIESVLRGQQGK
                     RVTLRLRSGQEITGSVRMSNARVVQLGAISGREFFDAVVPLEAVDAVLIRTKE"
     gene            complement(2467..3225)
                     /locus_tag="AC16CX_15370"
     CDS             complement(2467..3225)
                     /locus_tag="AC16CX_15370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3013922.1"
                     /translation="MAAGSRTALVTGAGTGVGKAVALAFLRDGYRVALAGRRKELLEQ
                     TAAEAGANGKNALIVPTDVGKPGEVKALFAKIKEKFGRLDVLFNNAGGNAPGVAFEDL
                     SYEKWQEVVDVNLTGMFLCAQAAFRMMKDQDPRGGRIINNGSISAHAPRPGSAPYTAT
                     KHGVTGLTKTIALDGRKHDVACGQIDIGNAITPMTERMAKGVMQANGTVSPEPRMDAA
                     HVGSAVLFMANLPLDVNVLTMTIMATKMPFVGRG"
     gene            3414..3599
                     /locus_tag="AC16CX_15375"
     CDS             3414..3599
                     /locus_tag="AC16CX_15375"
                     /inference="COORDINATES: protein motif:HMM:NF016473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HIR3013923.1"
                     /translation="MDLLTVMIMVAAFLTLVTLAGGVSAMATDGEIAHHTSGEWMFAR
                     VGAQALTLFLIGLSLIS"
BASE COUNT          594 a         1289 c         1273 g          677 t
ORIGIN      
        1 gacgcgtgaa tccacgcgcg ccatgaagcc gaggaagatc gcggcgagtt cggcttcgcg
       61 cgcgtcccat tcctcgcgcg gggcgacgtc gagatcgtag tgcttggtaa tgagcgggtg
      121 cacttccgcg tcgcgcatca gccggcccag gttctcggcg gcgctgcgca ggtggctcag
      181 gtcattgccc gggacgatgc aggccggggc gcggatcgtc ttgagctcgg cctcggtcgc
      241 gccgatcacg ggcttgcccg catcgtcgag gaatttgcgg ttccagttgg acatgacctc
      301 gatgaagcgt tggggatcca tcttcatcaa ccgctcgcgg ttctcgggcc gttcccggat
      361 ccgctcgctg aactcctcgg tcgcgcagac cgccgccatg ccgccctgtt gcgcgatcgg
      421 gaggtactgg ccgtagtaac ggtgcgccag gcgcttcgcc gcggcgaggc cgccggtaac
      481 gcgccacagc agcagtccgc gcacagcttg cgggtggcgc agggcgaaca gcagagcgag
      541 ccggcagccc gaggagctgc cgccgaccca ggcgggcagg gcattctgcc gcctgagcag
      601 ctcgtgcaag tcgtccgccc agatctcgtt ctcggattca ttgccctcga tcacgacgtc
      661 ggaagcgccg cagttgcgcc ggtcgtgcac cagcacgcga tatcctgcgc cggcgatccg
      721 cctcgcgagc gattccacgc cctcgatcgc ccgccgcccg ccgggcgaca gcgtgaccca
      781 cgggcccttc gtgccgagta cttcataatg gatggagacg cctcgtacgt ttgcgatggg
      841 catggatgac tccgattgag gagaaggcgt gggttgtggg tcgagggtcg acgagaagcc
      901 gggttaaggt tccggaatca cgacccacga tccacgatct tccggcccgc agggccgaga
      961 taagtgcagg actgccccag cgaatcgcgg cggacggtga cgcgcgagag gccggggaac
     1021 ttgcccgaaa gccgttccca catccacagg cacaggttct cgaggctggg tttcttgagg
     1081 cccttgatct cgttgagcag cgcgtgatcg agcctgcggt gcagtccgct gcaggctcgt
     1141 tcgagccggg cgaagtcggc gacgaaaccg tgcggcgggc gcggcgcgcc gcggagcgcg
     1201 acctcgacgc gaaacgagtg tccgtgcacg ccgcggtagc ggtggccgcg cggcatgtcc
     1261 ggaaaacgat gcgcggcatc gaagccgaat tcgtagcgga tctccatggc cggggatccg
     1321 gcggtccgtc cgcgggcgcg cctcatcacg gaatgccgat cagcttgtgg acctggagcg
     1381 agagccgcca gcgcggatgc cgcaggcaat agccggcggc gagccgcgtg tttcggacgc
     1441 gatacgggcc gtccatcggt tgcaggaaga agtggtcgaa gtcgaggccc tcgtagcggt
     1501 ccggcccggc gccccgctgc ggatagacga gcttcaactc gtcgccgcgg gtgaggcgca
     1561 gcggcgcgcg cgccttcggg ctcacgcaga tccagtcgat gccgggcggc ggctcgagcg
     1621 tgccgttggt ctcgatggcg acgcggaaac cccgcgcgtg cagcgccttc accagcggcg
     1681 cgtcgagctg caacagcggc tcgccgccgg tgcagacgac gaagcgcggt gccgccgcgc
     1741 gcttcggcca tttcgcctcg accgcgttcg cgagcgcgcc ggcgctgcgg aacttgccgc
     1801 cgtcctcgcc gtcggtgccg gcgaagtcgg tatcgcaaaa gcggcatatc gctttcgcgc
     1861 gatccttttc gcggcccgac cagaggttgc agccggcgaa gcgcaggaac acggcgggca
     1921 tgcccgtgtt ggcgccttcg ccctgcagcg tgtagaagat ttccttcacc gagtacataa
     1981 gtcgaaaagc gtgagtcgtg attcgtgggc ccgtcccaaa gttggcagca actgcaattt
     2041 gcccactgca aactgcaaac tattcatccc tcatccctcc gccctcatcc ctcgtcactc
     2101 cttggtgcgg atcagcacgg cgtcgaccgc ctccaggggc acgaccgcgt cgaagaattc
     2161 ccgtccgctg atcgcgccga gctgcaccac gcgggcgttg ctcatgcgta cgctacccgt
     2221 gatttcctgg ccggagcgca ggcgcagcgt gacccgcttt ccctgctgcc cgcgcagcac
     2281 cgattcaatc gtgtcgcccg agccgacctg aagctccgcg ctcgcggcag gcgcgacagc
     2341 gcacgcgagg accgtggcaa tcagcaatgc agaagcaatt tgtcgcatgt tgtttctccc
     2401 ttgaagtggc aaatcgcgag tttgtccctc acttctccgc cctcagccct catccctggc
     2461 cgtgcgtcag ccgcgcccga cgaagggcat cttggtcgcc atgatggtca tggtgagcac
     2521 gttcacgtcg agcggcaggt tggccatgaa cagcacggcg ctgcccacgt gcgccgcgtc
     2581 catgcgcggt tcgggggaga cggtgccgtt cgcctgcatg acgcccttcg ccatgcgctc
     2641 ggtcatcggg gtgatggcgt tgccgatgtc gatctgcccg caagcgacgt cgtgcttgcg
     2701 cccgtcgagc gcgatggttt tggtgagccc ggtcacgccg tgcttggtcg cggtgtaagg
     2761 cgcggagccc ggccgcggcg cgtgcgcaga gatcgagccg ttgttgatga tgcgcccccc
     2821 gcgcgggtcc tggtccttca tcatgcggaa cgccgcctgc gcacacagga acatgccggt
     2881 gaggttcacg tccacgactt cctgccattt ctcgtagctc aggtcctcga acgccacccc
     2941 gggcgcgttg ccgccggcgt tgttgaacag cacgtcgagg cgtccgaatt tttccttgat
     3001 cttcgcgaac agcgccttca cctcgcccgg cttgccgacg tcggtcggca cgatgagcgc
     3061 gttcttcccg ttcgcgccgg cttccgccgc cgtctgctcg agcagttcct tgcgccgccc
     3121 cgcgagcgcg acgcggtagc cgtccctgag aaacgcaagc gcgaccgctt tcccgacccc
     3181 cgtgccggcg ccggtgacga gcgcggtcct gctgccagcc gccatcgttc agcctcccca
     3241 agccattgag aaagcgtgca tgttaaccca ccttccagcc gggcgtgaac gtcgaaaacc
     3301 ggcttgccgg cgggcctttc gttccggaaa catggccttt tattgacctt cgtcaaactc
     3361 cgccggggcg tcaagcgcat catgaaaccg ctttatctga aaaggagggg tttatggatc
     3421 tcctcaccgt gatgatcatg gtagcggcgt tcctcaccct ggtgaccctc gccggcggtg
     3481 tctccgcgat ggccacggac ggcgagatcg cccatcacac cagcggcgag tggatgttcg
     3541 cgcgggtcgg ggcgcaggcg ctgacgttgt tcctcatcgg gctctcgctg atctcgtaaa
     3601 gccgcttgca tcccgggtcc gcctggtccc gggagttcta cgccggacgg ccgttgtctg
     3661 cttgacgaca gcgccgtgcc ggcatcctga gttgccgatt cctttaattg aaggcggagc
     3721 ggttcgttac gccgacgcgg gatgaatccc ggcactgcgc ggccgcgcga gcgaggcgtc
     3781 actgcccggg gaagcggcgc acgaggaagt cgcgcagcgc aagccccgcg atc
//
LOCUS       DBNGWZ010000501         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2964832, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000501 DBNGWZ010000000
VERSION     DBNGWZ010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2964832"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="AC16CX_15380"
     CDS             <1..734
                     /locus_tag="AC16CX_15380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013924.1"
                     /translation="APPDGYTILFNTLAAFTQNTSYYPKLPYDPAGFTLVAQVGWTPI
                     MMLAHPSLPVKTVRDVIALAKAHPGELTYGSFGRGSSSHFAGALLAYETKTNVIHVPY
                     KGGGPVAAANIAGEVPIHYGGVPPTIQFVKAGRLKALAVTAAKRTAFLPEVPTIAEAL
                     KIRDYDITVTFGMLVPAGTPKSITDRLHAEINKIVGSADFRGRLKLLGTDETAVMTLV
                     ELSAWLRNETARWKRIIEFTGIRAD"
     gene            834..2000
                     /locus_tag="AC16CX_15385"
     CDS             834..2000
                     /locus_tag="AC16CX_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3013925.1"
                     /translation="MRITDIRETAVPIKSNLRNAAFDFSEMTTSVVAVITDVMRDGAP
                     VAGFAFNSTGRYACGAQMRDRFIPRILKAPPESLVNSRGDNLDPEKILMCMMSREKPG
                     GHAERSIAVGAIEVAVWDAVAKIAGKPLHRVLAEKYNHGKSPKRVPCYVGGGWYEPGK
                     GLKELQDEIRAKLDQGYTTMKIKVGGASIPRDVERIEAALKVVRSSENLAVDANAAFD
                     RARALAYAKALAPYELRWFEEPCDPLDYALLVDVAHDYDAPLATGENLFSTQDVQNLV
                     SYGGLRADRDILQMDVPQSYGIVQFSRTLAMLERRGWGRKSVFPHGGNQMTLAIVGGF
                     GLGGCEAYPGVFGPFAGFADDAKVEGGYLKLSDRPGIGFEAQNALYAVMRELVD"
     gene            2175..2975
                     /locus_tag="AC16CX_15390"
     CDS             2175..2975
                     /locus_tag="AC16CX_15390"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3013926.1"
                     /translation="MSPKTLEILCSPLERAPLDLAVDASANGEVVQYLVNRERGLKFQ
                     IRDGIPIFVEPHQITGINSRFRNIYDNWAPFYDLLSRFGLYILGVSEVGLRKQFVGSL
                     SIKAGDRVLATSIGTGSDLPFLPRNCDYYGLDLSAGMLKICLKKMRKLGASAELFLGQ
                     AEHLPFLDESFDVVYQMGGINFFSDQVKAIQEMIRVARGGSKIVIMDETEKVARRLEH
                     IPGINAWFRHQHRPIVPPTTLIPAAMEQVVYRELYEGQAWYLSFRKPL"
     gene            complement(3162..3248)
                     /locus_tag="AC16CX_15395"
     tRNA            complement(3162..3248)
                     /locus_tag="AC16CX_15395"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3212..3214),aa:Leu,seq:caa)
     gene            3537..>3694
                     /locus_tag="AC16CX_15400"
     CDS             3537..>3694
                     /locus_tag="AC16CX_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR3013927.1"
                     /translation="MRRDDFDYELPQELIAQFPASERGGSRLLHLDGKTGALTDRQFR
                     DIVELVAPG"
BASE COUNT          703 a         1127 c         1185 g          679 t
ORIGIN      
        1 aagcgccgcc ggacggttac acgatcctgt tcaacacgct ggccgctttc acccagaaca
       61 cgagctacta cccgaagctg ccgtacgacc cggcgggctt cacgctggtg gcgcaggtcg
      121 gctggacccc gatcatgatg ctggcgcacc catcgctgcc ggtgaagacc gtgcgcgatg
      181 tcatagcgct cgccaaggcc catcccggcg agctgaccta cgggtcgttt ggcaggggca
      241 gcagctcgca tttcgccggc gcgctgctgg cgtacgagac gaagaccaat gtgatccacg
      301 tgccgtacaa gggcggcggc cccgtggccg cggcgaacat cgccggcgag gtgccgatcc
      361 attacggagg cgtgccgccc acgatccagt tcgtgaaggc gggcaggttg aaggcgttgg
      421 cggtgacggc ggcgaagcgc accgcgttcc ttcccgaggt gccgaccatc gctgaggcgc
      481 tgaagatccg cgattacgac atcaccgtga cgttcgggat gctggtgccg gcgggcacgc
      541 caaagtcgat cacggatcgg ctgcacgccg agatcaacaa gatcgtgggc tcggcggact
      601 tcagagggcg gctgaagctc ctgggcacgg acgagactgc ggtgatgacc ctggtggagt
      661 tgagcgcgtg gctcaggaac gaaaccgcgc gctggaagcg catcatcgag ttcaccggaa
      721 tcagggccga ttgattccgg tgagcggtaa gcggtcagcg gaaaggggta gctgctagcg
      781 gcccatactg ctcacggctt accgctcact gcttaccgct tacctgtatt ttcgtgcgaa
      841 taacggatat ccgcgagacc gcggtcccca tcaagtcgaa cctgcgcaac gcggcgttcg
      901 acttcagcga gatgaccacc tcggtggtgg cggtgatcac cgacgtcatg cgcgacggcg
      961 cgccggtcgc gggtttcgcg ttcaactcca cggggcgcta cgcgtgcggc gcgcagatgc
     1021 gcgaccggtt catcccgcgc atcctcaagg cgccgcccga gtcgctcgtg aacagccgcg
     1081 gcgacaatct cgaccccgag aaaatactca tgtgtatgat gagccgcgag aagccgggcg
     1141 gacacgccga gcgctcgatt gccgtcggcg ccatcgaagt cgcggtgtgg gacgcggtcg
     1201 ccaagatcgc cggcaagccc ctgcatcgcg tgctcgcgga aaagtacaac cacggaaagt
     1261 caccgaaacg agtgccctgc tacgtcggtg gcgggtggta cgagccgggc aaggggttga
     1321 aggagctgca ggacgagatt cgcgcgaagc tcgaccaggg ctacaccacc atgaagatca
     1381 aggtcggcgg cgcgagcatt ccccgggatg tggagcgcat cgaagcggcg ctgaaagtgg
     1441 tgcgttcgag cgagaacctt gccgtggatg ccaatgccgc attcgaccgc gcccgcgcgc
     1501 ttgcgtacgc caaggcgctt gcgccgtacg agttgcggtg gttcgaggag ccgtgcgatc
     1561 cgctcgacta cgcgctgctc gtcgacgtcg cccacgatta tgacgcgccg ctcgccaccg
     1621 gcgagaacct gttttccacg caggacgtgc agaacctggt gagctacggg ggcctgcgcg
     1681 ccgaccgcga tatcctgcag atggacgtgc cgcagtccta cggcatcgtg cagttctcgc
     1741 gcacgctcgc gatgctcgag cgccgcggct gggggcggaa gtcggtgttc cctcacggcg
     1801 gcaaccagat gacgctcgcc atcgtcggcg gcttcgggct gggcggctgc gaggcctatc
     1861 ccggcgtgtt cggtcccttc gccggcttcg cagacgacgc caaggtggag ggtggctacc
     1921 tgaaactgtc cgaccggccg gggatcgggt tcgaagcgca gaacgcgttg tacgcggtga
     1981 tgcgcgagct ggttgattga ccaccaagac cccaagaatg acaacactgg aaaagaggtc
     2041 gagtcttgta ttagcgtacg tcaattcaag acctgatcct tcattccgct tgaaacccgt
     2101 gggtcgtgat tcgtggatcg tgggtcgata aaatcgcgcg acacccaaat tgctgtcggg
     2161 accgctgacg aaagatgagc cccaagaccc tggaaatcct gtgcagcccg ttggagcgcg
     2221 cgccgctcga cctggccgtc gacgcgtccg ccaacggtga ggtcgtccaa tacctcgtca
     2281 atcgcgagcg tgggctcaag ttccagattc gcgatggcat cccgattttt gtcgagccgc
     2341 accagattac cggcatcaac agccggtttc ggaacatcta cgacaactgg gcgcccttct
     2401 acgatctatt gagccggttc gggctttaca ttctgggcgt gtcggaggtc gggctgcgca
     2461 agcaatttgt cgggagcctt agcatcaagg cgggcgaccg ggtgctcgcg acctcgattg
     2521 ggaccgggtc cgacctgccg tttctcccgc gcaactgcga ttactacggc ctcgacctgt
     2581 ccgcggggat gttgaagatc tgcctgaaga agatgcggaa gctcggtgcg agcgccgagc
     2641 tcttcctggg ccaggcggaa catctgcctt tcctggacga gtctttcgac gtcgtctacc
     2701 agatgggcgg aatcaatttt ttcagcgacc aggtgaaagc tatccaggaa atgattcgcg
     2761 tcgcccgcgg cggatcgaaa atcgtcatca tggacgaaac cgagaaggtg gcccgccggc
     2821 tggagcatat tcccggcatc aatgcctggt tcaggcatca gcaccggccg atcgttccgc
     2881 ccacgacgct gatcccagcg gcaatggagc aggtggtgta tcgcgagctg tatgaggggc
     2941 aggcctggta cttgagcttc cggaaaccgc tttgacgagt cgccacccgc aagtgacccg
     3001 gggaaaaacc agtggaaacg acccggagtc gccgtgatca gcgtaactct cgcctcgtga
     3061 aaatcgctcc gaccctgagg tctcatgagt gcggagcggt attgattctg cgaagaggtg
     3121 ctgattctgc attctgcact atgcactcat cactgaagtg atggtgccgg gagagagaat
     3181 cgaactctca tgccattgct ggcgcgggat tttgagtccc gtgcgtctac caattccgcc
     3241 atcccggcac tgcttccact tccgcaggcc ccggcgggat tttggctccg gccgtgcctg
     3301 cggcacttaa ccttcgcaag ctcaggttag cctgcgccgc aacaagccga ccgatattga
     3361 accgttatgc ttcgcttcac ggccactcat aatgtcggcg tgttgtgagt ccgctgcgtc
     3421 taccaattcc gccatcccgg caaggaatgc gcgattatcg cgcattcctt gagttgcgaa
     3481 caagaaaacc cggccaccag tcacgagtca cgagtcacgc ctcttataat gaggcgatgc
     3541 gccgcgacga tttcgactat gagctgccgc aagagttgat cgcgcagttt ccggcctcag
     3601 agcgcggtgg gagccggctt ctgcatcttg acgggaagac gggcgcgctg accgaccggc
     3661 agtttcgcga catcgtcgag ctggtcgcgc ccgg
//
LOCUS       DBNGWZ010000502         2767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1197387, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000502 DBNGWZ010000000
VERSION     DBNGWZ010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1197387"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..2034
                     /locus_tag="AC16CX_15405"
     CDS             280..2034
                     /locus_tag="AC16CX_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR3013928.1"
                     /translation="MTRNHRYSTLALAVLAALSLTVGAAFAQEKPAAKAPTKGKPPVS
                     DAEIRYQAGGSPLAKEEMHQDINPKAPQMTKAEFDRARQIYFERCAGCHGVLRKGATG
                     KPLTPDITLAKGTEYLKVFINYGSPAGMPNWGTGGELTTQEVDIMARYIQQTPPQPPE
                     WGLAQMKGTWKVIVPPEKRPKKQENKYNIDNIFATTLRDTGEVALIDGDTKQIINIVK
                     TGYAVHISRTSGSKRYVYTIGRDGKLNLIDLWMAKPDNVAEIRIGLEARSVETSRAKG
                     WEDKYAIAGAYWPPQYVIMKGDTLEPLKIVATRGMTVGDQEYHPEPRVASIVGSHSKP
                     EFVVNVKETGKILLIDYRDIANLKVTEIEGAKFLHDGGWDSTKRYFLVAANQSNKIAV
                     VDTKEGKLVKLIDVDKIPHPGRGANFEHPKFGPVWATGHLGSEKIALIGTDEHHKASA
                     WKVVQYLEGQGGGNLFIKTHPKSKNLWVDTPLNPDPKIAQSVAVWDIRKLDQKPEILP
                     IAEWAGLGEGAKRVVQPEYNKAGDEVWFSVWSAKNQQSALVVVDDKTRKLKTVIKDPR
                     IVTPTGHFNVYNTTHDIY"
     gene            2046..2375
                     /locus_tag="AC16CX_15410"
     CDS             2046..2375
                     /locus_tag="AC16CX_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013929.1"
                     /translation="MRKSLIAAWGAAALGIAVTGSAVAADVAKAEALLKQHNCTQCHG
                     IDKKILGPGYKDVAKKYKGQKDAAAMLAKKVKEGSKGVWGQIPMPPNDKVSDADIKTL
                     VEYILSL"
     gene            2386..>2767
                     /locus_tag="AC16CX_15415"
     CDS             2386..>2767
                     /locus_tag="AC16CX_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3013930.1"
                     /translation="MKPGAVDGAAERGERPYQLPHPFKLLLLAAAVFALTLAVTLSPA
                     ADETARPAEATVGPERQVELVRFVRHDCGSCHGLTLRGGLGPALTPAALGAISPDALA
                     ILILNGRHGTAMPGWRPILSDADVA"
BASE COUNT          576 a          882 c          909 g          400 t
ORIGIN      
        1 acccccggga aactttgatc tacatcaagg ttgcaggacc ccgcccgcgc catgctgtcg
       61 cccgatgcat aaggagtttg tcggtgcgcc gaaacgcgga gcagggcccc ggtttccggg
      121 gatgcgaccg tggaggcgca cggaagcacg cgtgcgatcg cggcgctgca tcccgctccg
      181 gggtcgcatc caagcacaaa tcgcttggaa cctgctccgc cctccgcggg cgctggcccc
      241 gacaaactcc tgatgatttc ctttctggga gcgcagccta tgacacgcaa ccatcgctac
      301 tcgaccctcg ccctcgcggt cctcgccgcg ctgtcgttga cggtcggcgc ggctttcgca
      361 caggagaagc cggcggccaa ggcgcccacc aagggaaaac cgccggtgag cgatgccgag
      421 atccgctacc aggccggcgg ctcgccgctc gccaaggagg agatgcacca ggacatcaat
      481 cccaaggcgc cgcagatgac caaggccgaa ttcgaccgcg cgcgccagat ctacttcgag
      541 cgctgcgccg gctgccacgg cgtgctgcgc aagggcgcaa ccggcaagcc cctcaccccc
      601 gacatcacgc tcgccaaggg aaccgagtat ctgaaggtgt tcatcaacta cggctcaccc
      661 gcgggcatgc cgaactgggg caccggcggc gaattgacga cccaggaagt ggacataatg
      721 gcgcgctaca tccagcagac cccgccgcag ccgccggagt ggggcctggc gcagatgaag
      781 ggcacgtgga aggtgatcgt gccgcccgag aagcgcccga agaagcagga gaacaagtac
      841 aacatcgaca acatcttcgc gaccacgctg cgtgacaccg gcgaggtggc gctcatcgac
      901 ggcgacacca agcagatcat caacatcgtg aagaccggct acgcggtcca catctcgcgc
      961 acctcgggct caaagcgcta tgtctatacg atcgggcgcg acggcaagct caacctgatc
     1021 gacctgtgga tggccaagcc tgacaacgtc gccgagatcc gcatcggtct cgaagcgcgt
     1081 tccgtcgaga cttcgcgcgc caagggctgg gaggacaagt acgcgatcgc gggcgcgtac
     1141 tggccgccgc agtacgtgat catgaagggc gacacgctcg agccgctcaa gatcgtggcg
     1201 acgcggggca tgacggtcgg tgaccaggag taccatccgg agccgcgcgt tgcctcgatc
     1261 gtcggcagcc attccaagcc cgagttcgtg gtgaacgtca aggagaccgg caagatcctg
     1321 ctgatcgatt atcgcgacat tgcgaatctc aaggtcacag aaatcgaagg cgcgaagttc
     1381 ctgcacgacg gcgggtggga ttcgaccaag cgctacttcc tggtggcggc caaccagtcg
     1441 aacaagatcg cggtggtgga caccaaggaa ggcaagctgg tgaagctgat cgacgtggac
     1501 aagatcccgc accccgggcg cggcgccaat ttcgagcacc cgaagttcgg cccggtatgg
     1561 gcgaccggcc acctcggcag cgagaagatc gcgctcatcg gcaccgatga gcaccacaag
     1621 gcatccgcct ggaaagtggt gcagtacctc gagggccagg gcggcggcaa ccttttcatc
     1681 aagacgcatc cgaagtcgaa gaacctgtgg gtggacacgc cgctcaatcc cgacccgaaa
     1741 atcgcgcagt cagttgcggt ctgggacatc cgtaaactcg accagaagcc cgagatcctg
     1801 ccgatcgccg aatgggcggg cctgggcgag ggcgccaagc gcgtggtgca gccggagtac
     1861 aacaaggccg gcgacgaggt gtggttctcg gtgtggagcg ccaagaacca gcaatccgcg
     1921 ctggtggtgg ttgacgacaa gacgcggaag ctcaagaccg tgatcaagga tccgcgcatc
     1981 gtgacgccga ccgggcactt caacgtctac aacacgacgc acgacatcta ctagaggagc
     2041 gaatcatgcg gaagagtctg attgcggcct ggggcgccgc cgccctggga attgcggtga
     2101 cgggcagtgc ggtcgccgcc gacgtggcga aagccgaggc gctgctcaag cagcacaact
     2161 gcacccagtg ccatggcatc gacaagaaga tcctcgggcc cggttacaag gacgtcgcca
     2221 agaaatacaa gggccagaag gacgctgcgg cgatgctggc gaagaaggtc aaggaaggca
     2281 gcaagggggt ctggggccag attccgatgc cgcccaacga caaggtgagc gacgccgaca
     2341 tcaagacgtt ggtggagtac atcctgtcgc tgtaggggac gagtggtgaa accgggcgcg
     2401 gtggatggag cggcggagcg tggagaacgt ccatatcagt tgccgcaccc attcaagctc
     2461 cttctgctcg cggcggccgt attcgcgctc acgctcgccg tgacgctttc gcccgcggcc
     2521 gacgaaacgg cgcggcccgc agaggcgaca gtcggccccg agcggcaggt ggagctcgtg
     2581 cgcttcgtgc gccacgattg cggttcttgt cacgggctga cgctgcgcgg agggctcggg
     2641 ccggcattga cgccggccgc gctcggcgcg atatcgccgg acgcgcttgc aatcctcatc
     2701 ctcaacggcc gacacggcac cgcgatgccc ggctggcgtc cgatcctgtc cgacgccgac
     2761 gtcgcgt
//
LOCUS       DBNGWZ010000503         2932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1326957, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000503 DBNGWZ010000000
VERSION     DBNGWZ010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1326957"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC16CX_15420"
     CDS             <1..542
                     /locus_tag="AC16CX_15420"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013931.1"
                     /translation="LARKNPGKLNYGSGGVGTTTHLTPELIMSITKTRMTHVPYKGSG
                     LALIALVGGQIDVLIMAVPAAAEQVKAGKARALLILSTERHPVLPNVPSAKDEGVVDY
                     IVQLWYGILTSAGTPANLVNRLNAEIIKAINSPDLRKRLVEGGVEPLTSTPEEFAKFI
                     QTETPRYAKIVKDANIPAQ"
     gene            663..1520
                     /locus_tag="AC16CX_15425"
     CDS             663..1520
                     /locus_tag="AC16CX_15425"
                     /inference="COORDINATES: protein motif:HMM:NF025091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3013932.1"
                     /translation="MKSPTIRQLAQTERPLVLPGAHDALSALLIKQAGFKGFFIGGFQ
                     TAGARYGLPDIGLAQLGEFTAAFRDIIGACDLPVLVDADNGYGDVKNCVHLLHTYERM
                     GVSAMFIEDQVSPKRCGHMAGKRVVPAEEMEAKLRAMAANRMNPDTFIVARTDARAIH
                     GMDEALRRAGRYVRAGADGVFIESMVNEEEIARAVHEIDTVHVANMLEGGITPILKPS
                     VLGEMGYKIALYGITLLLRITKTMQMALADLKSGELKLVGSGTPFEEYMKIVGIERWR
                     AIEQKFGQL"
     gene            1556..2530
                     /locus_tag="AC16CX_15430"
     CDS             1556..2530
                     /locus_tag="AC16CX_15430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013933.1"
                     /translation="MKSTINIGLTAVLSFFALCVSHAAEPQYPSRPVRIIVPFAPAGP
                     TDFVARIVAQKLSESWGQQFVVDNRSGAGGNIGMGIAASAAGDGYTLIVVSSSLMVNP
                     SLYKKVPYDPHKSFAPISNIAASPHVFFTHPSQPVKSIAELIAAVKKDPKKYSISSPG
                     IGTVPHLSSTLLSLDAKIDLVIVPYGGGGLSLAAVVGNQVPFGCQAIPPVTPHIKAGR
                     VRALAMTSEKRSEIVPDVPTMAELGFKGHEADTISGILATAGTPAAIVMKLHAETVKA
                     ILSPDVKARIAEQGFDIVLSSPQEFTEKIKREVAKWGKVIKDANIVVN"
     gene            2568..>2932
                     /locus_tag="AC16CX_15435"
     CDS             2568..>2932
                     /locus_tag="AC16CX_15435"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013934.1"
                     /translation="METTSRWILGIAALVAAGVAYAQSWPARPVRIIVPWAPGGASDI
                     IARPLAQKLSESLGQQFVVDNRGGANSLIGTDVAAKAPPDGYTILFNTLAAFTQNTSY
                     YPKLPYDPAGFTLVAQVGWT"
BASE COUNT          579 a          927 c          956 g          470 t
ORIGIN      
        1 cgctggcgcg caagaatccc ggcaagttga actacggttc cggcggggtc ggcaccacca
       61 cccacctcac gcccgagctc atcatgagca tcaccaagac ccggatgacg cacgttccgt
      121 acaagggctc gggcctggcg ctcatcgcgc tggtcggcgg gcagatcgac gtgctgatca
      181 tggcggtgcc cgcggccgcc gagcaggtga aggcggggaa ggcgcgcgcg ctcctgatac
      241 tctccaccga gcgccatccc gtgctgccca acgtgccgtc cgcgaaagac gagggcgtgg
      301 tggactacat cgtccagctg tggtacggga tcctcacgtc ggcgggcacg ccggcgaacc
      361 tcgtcaaccg gctcaacgcc gagatcatca aggcgatcaa ttcacccgat ctcaggaagc
      421 ggctcgtgga aggcggtgtc gagccgctca ccagcacgcc ggaggaattc gcgaaattca
      481 tccagaccga aaccccgcgc tacgccaaga tcgtcaagga cgccaacatt cctgctcagt
      541 agacattaaa tccggacgca ttcgcgccgg tttgaatcgc gcgcgatcga acgaacgtca
      601 gagccgtcgt tcatcgcgcg ccggaataac cggaacgcgt cacccatcac tcattaccag
      661 aaatgaaatc gccgaccatc cgccagctcg cgcagaccga gaggccgctg gtgctgccgg
      721 gcgcgcacga cgcgctctcg gcgctgctca tcaagcaggc cggcttcaag ggcttcttca
      781 tcggcggctt ccagaccgcc ggcgcgcgct acgggttgcc cgacatcggg cttgcccagc
      841 tcggcgagtt caccgccgcg ttccgcgaca tcatcggcgc ctgcgacctg ccggtgctgg
      901 tggacgccga caacggctac ggcgacgtaa aaaactgcgt gcacctgctg cacacctacg
      961 agcgcatggg cgtgtcggcg atgttcatcg aggaccaggt ctcgcccaag cgttgcggcc
     1021 acatggcggg aaaacgggtc gtaccggcgg aggagatgga ggcgaagctg cgcgcgatgg
     1081 cggcgaaccg gatgaacccg gacacgttta tcgtcgcccg caccgacgcg cgggcaatcc
     1141 acggcatgga cgaggcgctg cgccgcgccg ggcgctatgt gcgcgccggt gccgacggtg
     1201 ttttcatcga gtccatggtg aacgaggagg agatcgcgcg cgccgtgcac gagatcgata
     1261 ccgtgcacgt cgcgaacatg ctcgaaggag gcatcacgcc gatcctcaag ccctctgtgc
     1321 tcggtgagat gggctacaag atcgcactct acggcatcac gctgctgctg cgaatcacga
     1381 agacgatgca gatggctctg gcagacctga agagcggcga gctgaagctc gtcggctcag
     1441 gcacgccgtt cgaggaatac atgaagatcg tgggcattga gcgctggcgc gcgatcgagc
     1501 agaagttcgg ccagttatag catcagcgac gagtgacgaa aacccaggag ggaacatgaa
     1561 atccactatt aatatcgggt tgaccgcggt gctgtcgttt ttcgccctgt gtgtttcgca
     1621 cgcggcggag ccgcagtacc cctcgcggcc ggtgcgcata atcgtgccgt tcgcgcccgc
     1681 cgggccgacc gatttcgtgg cccgcatcgt ggcgcagaag ctgagcgaga gctgggggca
     1741 gcagttcgtg gtggataatc gcagcggcgc cggcggcaac atcggcatgg gcatcgccgc
     1801 gagcgcggca ggagacggtt acaccctgat cgtcgtgagc tcgagcctga tggtcaatcc
     1861 gagcctctac aagaaagtcc cgtatgaccc gcacaagagt ttcgcgccga tctccaacat
     1921 cgcggcgtcg ccccacgtgt tcttcacgca cccgagccag ccggtgaaat cgattgccga
     1981 actgatcgcc gcagtgaaga aggacccgaa gaaatacagc atatcatcgc cgggaatcgg
     2041 gacggttccc cacctctcgt caaccctgtt gagtctcgac gccaagatcg acctggtgat
     2101 cgtgccctat ggcggcggcg ggctgtcgct tgcggcggtg gtgggcaacc aggtgccctt
     2161 cggctgccag gcgattccgc ccgtgacgcc tcacatcaag gctggccggg tgcgcgcgct
     2221 ggcgatgacg agcgagaagc ggtcggaaat cgtacccgat gtgccgacca tggcggagct
     2281 cgggttcaag ggccatgagg cggacacgat tagcggcata ctggcgacgg ccggaacacc
     2341 ggccgcgatc gtcatgaaac tgcacgccga gacggtcaag gccatcctgt cgcccgatgt
     2401 gaaggcgcgg atcgcggagc agggattcga catcgtcctg agttcacccc aggaattcac
     2461 cgagaagatc aagcgggaag tcgcgaagtg gggcaaggtc atcaaggacg ccaatatcgt
     2521 tgtgaattaa gagcgttcag atcgggcttt ttttcgagga ggggacgatg gaaacaacca
     2581 gcaggtggat actggggatc gcggcgcttg tcgcggcggg cgtggcctac gcccagagct
     2641 ggccggcgcg gccggtgcgc atcatcgtgc cgtgggctcc cggcggggcg tccgacatca
     2701 tcgcccggcc gctcgcgcag aaactttccg agagcctcgg ccagcagttc gtcgtggata
     2761 accgcggcgg cgccaacagc ctgatcggaa cggacgtcgc cgccaaagcg ccgccggacg
     2821 gttacacgat cctgttcaac acgctggccg ctttcaccca gaacacgagc tactacccga
     2881 agctgccgta cgacccggcg ggcttcacgc tggtggcgca ggtcggctgg ac
//
LOCUS       DBNGWZ010000504         3243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_193998, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000504 DBNGWZ010000000
VERSION     DBNGWZ010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_193998"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="AC16CX_15440"
     CDS             complement(<1..965)
                     /locus_tag="AC16CX_15440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3013935.1"
                     /translation="MFLPELSIRRPVLATVMSLAILLLGVISFLRLSVREYPKIDTPV
                     VSVRTVYTGASAQVIESQVTQPLEDSLSGIEGIRTIKSVSREEVSQITVEFIPERNVD
                     AAANDVRDRVARVRGLLPDEVDEPVVSKIEADAQAILWIAFYSDRHDALFITDYADRY
                     VADSLKALPGVASVIMGGARKYAMRIWVDRDRLAGFGLTPLDVENALRRQNIEIPSGR
                     IESRKREFTVLTETDLRTPDQFNNMIITEARGYPVRLKDIGRAELGAQDERSVVRVNG
                     NPAMGLGIVKQSTANTLEVAQAVKAQLPRIQSSLPEGMKTFIAFDS"
     gene            complement(1243..2337)
                     /locus_tag="AC16CX_15445"
     CDS             complement(1243..2337)
                     /locus_tag="AC16CX_15445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3013936.1"
                     /translation="MQKISTILTSLGGIALSGLLTFTAVVQAQQPKPKPMSLPVKAAP
                     VKVGTVTHDVTAVGTLLANESVMIRPEVAGRVAAIHFNEGQAVAARARLVSLDATEVQ
                     AQLAASRADERLTEQRAERAAELFKKNFISQQALEDAREAYKKATAQRQENEARVAKT
                     EIRAPFAGIVGLRQVSAGAYLKAGEDIVRLDKIDVMKLDFRVPEVYLGKIRRDQPVAV
                     RVDAFPGEQFGGRVYAVETAVDERTRTVQLRGRVENKGLKLRPGMFARVVLELGSNDK
                     AALIPEQAIVPRGNQNYVFRVVDGKAALTEVALGARSPGEVEIVRGLKVGEIVVTDGQ
                     LKLQDGTPVMVLPDKPAPTAATTKGNQKQN"
     gene            2447..>3243
                     /locus_tag="AC16CX_15450"
     CDS             2447..>3243
                     /locus_tag="AC16CX_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3013937.1"
                     /translation="MPLSLDALLVLDSIDRKGSFAAAADELHRVPSAITYTVQKLEED
                     LDVLLFDRRGHRAKLTAAGRELLNEGRHLLRAAGELESRVKRVATGWEAELRIAYDDL
                     IPARSILRLAGEFYREQCSTRLRISAEILGGCWDALATERADLVIAAPGDGPSGGGYS
                     AREMGSVEWVFAVAPAHPLASAPEPLKREDILKHRVAVVADSSRNLPPRTAGLLGGQE
                     LLTVHSLQAKLEAQVMGLGAGNLPRFLAEREAAAGRLVVKRTEEGKNT"
BASE COUNT          558 a         1064 c         1040 g          581 t
ORIGIN      
        1 gaatcgaagg cgatgaaagt tttcatgccc tccggcagcg acgactggat gcggggcagc
       61 tgcgccttca ccgcctgcgc cacctccagc gtgttggcag tggactgctt gacgatcccc
      121 agccccatcg ccggattgcc gttcacgcgc acgacgctgc gctcgtcctg tgctcccagt
      181 tcggcgcgcc cgatgtcctt caatcggacc ggatagccgc gcgcctcggt aatgatcatg
      241 ttgttgaact ggtcgggtgt ccgcagatcg gtctcggtca gcaccgtgaa ttcgcgcttg
      301 cggctttcga tgcggcccga gggaatctcg atgttctggc gccgcagcgc gttctccacg
      361 tcgagcggcg tcaatccaaa gccggcaagc cggtcgcggt ccacccagat gcgcatcgcg
      421 tacttgcgcg cgccgcccat gatgacgctc gccaccccgg gcagcgcctt gagggaatcc
      481 gcgacgtagc ggtcggcgta gtcggtaatg aagagggcgt cgtgccggtc gctgtagaac
      541 gcgatccaca ggatcgcctg cgcgtcggcc tcgatcttgg acacgaccgg ctcgtccacc
      601 tcgtcgggca gcaacccgcg cacccgcgcc acgcggtcgc gcacatcgtt cgccgcggcg
      661 tccacgttgc gctccgggat gaactccacc gtgatctgcg agacttcctc gcgcgaaacc
      721 gacttgatgg tgcggatgcc ctcgatgccg gagagcgagt cctcgagcgg ctgggtgacc
      781 tgcgactcga ttacctgggc gctggcgccg gtgtagacgg tgcgcaccga caccactggc
      841 gtgtcgatct tcgggtactc gcgcaccgac aggcgcagga acgagatgac gccgagcagg
      901 aggatggcga ggctcatcac cgtcgcgagg acggggcggc ggatggaaag ctcaggcaga
      961 aacatttaac cgccgagaca agataaaaat aaaatcaaac cctaatcgaa ccgccatgac
     1021 gccaagagaa gcacggcgaa aataagatca agaaccaatc gaaccgcaga gcacgcagag
     1081 agaaacaata cgaaaaataa aatcgcatcg gccctttttc cccaaccagc gaatttcggc
     1141 cattggttgt agtcgattgc agggtcgttc acttggttca attattgaat ctctctgcga
     1201 tctctgcggt tcaatatttg attctcttgg cgcctcggcg gttcaatttt gtttttgatt
     1261 tcccttggtg gttgcggccg tcggcgccgg cttgtcagga agcaccatca ccggcgttcc
     1321 gtcctgcagt ttgagctggc cgtcggtgac gacgatctcg ccgaccttga ggcccctgac
     1381 gatctcgacc tcgccggggc tccgggcgcc gagcgcgacc tcggtgagcg cggccttgcc
     1441 gtccaccacg cggaacacgt aattctggtt gccgcgcggc acgatcgcct gctccggtat
     1501 cagcgccgcc ttgtcgtttg acccgagctc gagcacgaca cgcgcaaaca tcccggggcg
     1561 cagcttcagg cccttgtttt ccacgcgccc gcggagctgc acggtgcgcg tcctctcgtc
     1621 caccgcggtc tcgaccgcgt acacccgtcc cccgaactgc tcgccgggaa acgcgtccac
     1681 ccgcaccgcg accggctggt cgcggcggat cttgccgaga tagacctcgg gcacgcggaa
     1741 atcgagcttc atcacgtcga tcttgtcgag ccgcacgatg tcctcgccgg ccttgaggta
     1801 ggcgccggcg ctcacctggc gcaggccgac gatgcccgcg aacggggcgc ggatttctgt
     1861 cttggcgacc cgtgcctcgt tttcctgacg ttgcgctgtg gccttcttgt acgcctcgcg
     1921 cgcgtcttcg agcgcctgct ggctgatgaa attcttcttg aaaagctcgg ccgcccgctc
     1981 cgcgcgctgt tcggtgagcc gctcatcggc cctgctcgcg gccagctggg cctgcacctc
     2041 ggtcgcgtcg agcgaaacca gccgcgcgcg ggccgccacc gcctggccct cgttgaaatg
     2101 aatcgccgcc acccgacccg cgacctcggg acggatcatg accgattcat tcgcaagtag
     2161 cgtcccgacc gcggtgacat catgggtgac cgtgccaacc ttgactggag cggccttgac
     2221 cggcaggctc atcggcttcg gcttgggttg ctgcgcctgg acgacggccg tgaacgtcag
     2281 gagtcccgat aacgcgatcc cgcccaaaga cgtcaggatc gttgatattt tttgcatgcc
     2341 tgctcgggag cggagccgcc cggggaaggt gaaaggcgtc attctaaatt tgaaccggcg
     2401 gaaaagatag acaataatcg gggcggtatc gttcaaagtt tttcacatgc cgctgtctct
     2461 cgacgcgctg ctggtgctgg actcgataga ccgcaaggga agctttgcgg ccgcggcgga
     2521 cgagttgcat cgcgtgccct ccgccatcac ctacaccgtg cagaagctcg aggaggacct
     2581 cgatgtcttg ctgttcgacc ggcgcggcca ccgcgcgaag ctcacggcgg cgggacgcga
     2641 gctgctcaac gaaggccgtc acctgctgcg cgccgcgggc gagctcgagt cgcgcgtgaa
     2701 gcgcgtcgct accgggtggg aagcggagct gcgcatcgcg tacgacgacc tgataccggc
     2761 ccgcagcatc ctgcgactcg ccggggagtt ctaccgcgag cagtgctcga cgcggctccg
     2821 catcagcgcc gagattctcg gcggctgctg ggatgcgttg gcgaccgagc gggcggacct
     2881 cgtcatcgcg gcgccgggcg acggtccttc gggaggtggc tacagcgcgc gcgagatggg
     2941 cagcgtggag tgggtgtttg cagtcgcgcc cgcgcacccg ctcgcctccg cacccgagcc
     3001 gctcaagcgg gaagacatcc tcaaacaccg agtcgctgtg gtcgcggaca gctcgcgcaa
     3061 cctgccgcca cgcactgcag ggctgctcgg cgggcaggaa ctgctcacgg tgcacagcct
     3121 gcaggcgaag ctcgaggcgc aggtgatggg actcggcgcg ggcaatctgc cgcgcttcct
     3181 ggccgagcgc gaagccgccg cggggcggct cgtcgtgaag cggaccgagg aggggaaaaa
     3241 tac
//
LOCUS       DBNGWZ010000505         5456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1829880, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000505 DBNGWZ010000000
VERSION     DBNGWZ010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1829880"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="AC16CX_15455"
     CDS             complement(<1..1522)
                     /locus_tag="AC16CX_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3013938.1"
                     /translation="MKKIWINSYPPGVPAKVDVHAFPSLKHLFARSCTRFADRPALTN
                     MGVSMSYAGLERLSRDFAAYLQHGLGLSRADRVAIMMPNLLQYPVALFGALRAGLTVV
                     NVNPLYTASELEHQLRDSGASAIVILENFAHNLEPALAGTGVRHVITTRIGDLFPLPK
                     RLLVNFAVKHVKRLVPAWHIPGATGFRDALSRGARLKLDEVPLGPEDIAFLQYTGGTT
                     GRPKGAILTHGNLVANVEQTAVWISASLKEGEETVITALPLYHVFALTANLLVFVKLG
                     GCNALITNPRDIPGFIAELKRTRFTAITGVNTLYNALLNAEGFEAVRAVNGNALKVAV
                     AGGMAVQRAVAERWQRLMGIPLIEGYGLTEASPIVCANRLDIQTYTGKIGLPVPSTEV
                     AILDEREAEVALGETGEICVRGPQVMRGYWNAPEETAAVFTADGLLRTGDLGRMDARG
                     YVEFVDRRKDVIVVSGFKAYPAEIEDVAMLHPGVKDAGAVGVPDERSGEAVVLFVVK"
     gene            complement(1756..2112)
                     /locus_tag="AC16CX_15460"
     CDS             complement(1756..2112)
                     /locus_tag="AC16CX_15460"
                     /inference="COORDINATES: protein motif:HMM:NF018534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013939.1"
                     /translation="MLVCLATIGATAHPVAAATIVIASPAHEETIHDNTGNVPVSVTI
                     RGAAPAGARIRALLDGRPYGTDQRSASFTLTQVERGEHTLQVQLVNPAGRVVASSATV
                     TFYLWQASRLFPSRKP"
     gene            complement(2166..2444)
                     /locus_tag="AC16CX_15465"
     CDS             complement(2166..2444)
                     /locus_tag="AC16CX_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR3013940.1"
                     /translation="MNAPTEKLVSDVKVLASDVEELLKATATQTGEKVAEARVRAQAA
                     IARAKTVAVEQGREAARATDQYVRQNPWSAIGISAGIGLLIGLLIGRR"
     gene            2701..4800
                     /locus_tag="AC16CX_15470"
     CDS             2701..4800
                     /locus_tag="AC16CX_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3013941.1"
                     /translation="MLARAADRQLATQNILNKVSRRAILAAGIALILVLVVATVLVLI
                     VLRNDALERAGWHLGNLATALGEQTWQSVHTLEVIGKTTADELSDRYTGKRSLSDREL
                     YERMKERIGALPNARMLAFIDPRGNSVLHSTAFPTPTANFAEHEHFQVHLKRRVEGLY
                     VGEPVFGRLMPEWLNVFSCRVEDGDGRFRGVVFLAVSMTYFQSLFGALDLGDGGRVFL
                     FRDDGVLLTTYPPEERAYGRSFAGDALFAGAPTRAAASYAERRAGLLDAAPRIVAHDH
                     LKGYPLVVAVSSTEDFILGAWRRDVWRIGAGAAGTVAFIGIVLYFLLRQLRVSVGLEN
                     ELRETGERLQGIIRSAMDAIITVDERQNIVMFNAAAEKIFRCSGAEAIGGPLDRFIPE
                     RFRAAHRRHVWRFGETGETTRMMGARLALFGLRSDGEEFPIDASISQISIEGRKLYTV
                     ILRDITRRKIAEDALERSYRELRELSATMNEVREAERLRIARELHDELAQWLTAIKMD
                     VSWLSSRLPREHAHLVDKTEKMKAVVDTTVAAVRRIAADLRPVMLDDLGLVAALEHLL
                     HDLSQRTGIIVSLEADAGELDLGEPLATSLYRMAQEALTNVARHAEATQVQLALWVED
                     GRLVLTVRDNGKGIDAAALGRRKSYGVLGIRERAHTLGGSARVERLATGGTLVEIVIP
                     VTRYRGREAADDTGVAG"
     gene            4778..5431
                     /locus_tag="AC16CX_15475"
     CDS             4778..5431
                     /locus_tag="AC16CX_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013942.1"
                     /translation="MIRVLLADDHTIVRAGLKEILADTGDITVAAEATNGQEVMAQVR
                     GQDFDVAVLDMSMPGRSGIELIKQVKDEKPRLPILILSMHSEEQYAVRALKAGASGYL
                     TKDAAADQLVAAIRRIAAGGAYVSPETAERLALDAGSRPEAAPHTLLSDREFQVFLMI
                     ARGVSVSEIARSLSLSVKTVSTHKTRILEKMGLANQAELIRYALEHQLLDEPPGSSR"
BASE COUNT          938 a         1712 c         1867 g          939 t
ORIGIN      
        1 ccttcaccac gaacagcacc actgcttcgc cggaacgctc gtccggcacc cccaccgcgc
       61 cggcgtcctt cacgccgggg tgcagcatgg cgacgtcctc gatctcggcc gggtaagcct
      121 tgaatcccga caccacgatc acgtccttgc gccggtccac gaactcgacg tagccgcgcg
      181 catccatgcg gccgaggtcg cccgttcgca gcaacccgtc cgccgtgaac accgcggccg
      241 tctcctcggg cgcgttccag tagccgcgca tcacctgcgg gccacgcacg cagatctctc
      301 cggtttcgcc cagcgcaacc tcggcttcgc gctcgtcgag gatcgcgacc tccgtcgagg
      361 gcacgggcag gccgatcttg ccggtgtagg tctggatatc gagccggttg gcgcacacga
      421 tcggagaggc ctcggtgagg ccgtagccct cgatgagcgg aatccccatc aagcgttgcc
      481 agcgttccgc gacggcccgc tgcaccgcca tgccgcccgc gaccgccacc ttgagggcat
      541 tgccgttcac tgcccggacc gcctcgaagc cttccgcatt gagcagcgca ttgtagaggg
      601 tgttgactcc cgtgatcgcg gtgaagcggg tgcgcttgag ctccgcgatg aagccgggaa
      661 tatcccgcgg gttggtgatc agcgcgttgc agccgcccag cttcacgaac accagcaggt
      721 tcgcggtcag cgcgaacacg tggtagagcg gcagcgcggt gatcaccgtc tcttcgccct
      781 ccttcaggct ggcgctgatc cagaccgcgg tctgctcgac gttcgcgacc aggttgccgt
      841 gggtcaggat cgcccccttc gggcggccgg tggtgccgcc ggtgtactga aggaacgcga
      901 tatcttccgg tccgaggggc acctcgtcga gcttgaggcg cgcgccgcgt gaaagcgcgt
      961 cgcgaaagcc ggtggcgccg ggaatatgcc aggccggcac caggcgcttg acgtgcttga
     1021 cggcgaagtt gacgagaagc cgcttgggca aggggaacag gtccccgatc cgggtggtga
     1081 tcacgtgccg gacgccggtg ccggcgagcg ccggctcgag gttgtgggcg aaattctcga
     1141 gtatgacgat cgccgacgcg ccggagtcgc gcagctggtg ctcgagctcg ctcgcggtgt
     1201 agagaggatt gacgttgacc acggtcaggc cggcgcgcag ggccccgaac agcgcgacgg
     1261 ggtattgcag caggttcggc atcatgatcg ctacgcgatc cgcccgggag aggccgaggc
     1321 cgtgctgcaa gtaggcggcg aagtcgcgcg acaggcgctc gagtcccgcg tagctcatcg
     1381 aaacacccat gttggtgagc gcgggccggt ccgcgaagcg cgtgcagctg cgcgcgaaca
     1441 ggtgcttgag ggagggaaaa gcgtgaacgt cgaccttcgc cggcaccccc ggcgggtagg
     1501 aattgatcca gatttttttc atgcgctggg acggcccgga acgccgcccg gaggaaccgc
     1561 tagcagcctg tggagcttaa cagtccgcac aggctgctga cagcaaaacc ggctgaccct
     1621 tcagcaaaaa acccggccga aggggtcgaa tgtgtagaaa agcgaaggtg atattgcata
     1681 gtcatcagcc cctgttgcaa ttttccaggt gccggttttg catcaggagt ttgtcggggc
     1741 cggcccgaca aactcctagg gcttgcggct cgggaacagc cgcgacgcct gccacaggta
     1801 gaacgtcacg gtcgcagagg atgcgaccac gcgcccggca gggttcacca gctgcacttg
     1861 caaggtgtgt tcgccgcgtt ccacttgcgt caacgtgaac gaggcggatc gctgatcggt
     1921 gccgtacggc cggccgtcga gcagcgcgcg gattcgcgcg ccggccggtg cagccccccg
     1981 tatggtgacc gatacgggca cgttcccggt attgtcgtgg atagtctcct cgtgcgccgg
     2041 gctcgcaatg acgattgtcg ccgccgcgac cggatgggcc gttgcgccga tcgtcgcgag
     2101 gcagacgagc acagcgactc ggacccccca tattcgaagg gaggccccgc cgcggctcat
     2161 ggcgctcagc ggcgtccgat cagcaatccg atcagaagac cgattcccgc cgatatgccg
     2221 atggcgctcc acgggttctg acggacgtac tggtcggtcg cgcgcgcggc ttctctgcct
     2281 tgctcgaccg caacggtctt ggcgcgggcg atagccgcct gggcccgcac acgcgcctcg
     2341 gcgaccttct cgccggtctg ggttgcggtt gccttcagca gttcttcaac gtcgctcgcc
     2401 aggactttca cgtcgcttac gagtttttcg gtgggtgcgt tcatggtttg ctcctgtcaa
     2461 ggaaagtgcg actcactcga cccgtcatac cgggtacggg tacgcctaca ctctagatgt
     2521 ttggacaagc cacctgttga tttggatcaa tgaatctgcg ggcggttttg ctgttggcgg
     2581 cctgtcggac acgcaagtcc gacaggctgc tggtggcgag gcgccgaccg gccctgacgg
     2641 cggcgggagc gcccgcaggg ctgcgctata ctaactgagg attttcagag agaaaccggc
     2701 gtgcttgcgc gcgccgcgga ccgccaattg gcaacgcaga acatcctcaa caaggtctcg
     2761 cgccgcgcga tactcgctgc gggaattgcg ctcatcctcg tgctggttgt cgcgacggtc
     2821 ctcgtgctga tcgtgctgcg aaacgatgcg ctggagcgcg cgggatggca tctcgggaac
     2881 ctggcaacgg cactcggcga gcagacctgg cagtcggtgc atacgctcga ggtcatcggc
     2941 aagaccacgg cggacgagct gagcgatcgt tacacgggaa aacggtcgct atcggaccgg
     3001 gagctctacg agcgcatgaa ggaacgcatt ggtgcgctgc ccaacgcgcg catgctggcc
     3061 ttcatcgatc cgcgcggcaa ctccgttctg cacagcacgg cttttccgac gccaacggcc
     3121 aacttcgccg aacacgaaca cttccaggtg cacctcaagc ggcgcgtcga ggggctgtac
     3181 gtcggcgaac cggtattcgg caggctgatg ccggaatggc tgaacgtgtt cagttgtcgc
     3241 gtcgaggatg gggacggccg gttcaggggg gtcgtcttct tggcggtgag catgacctac
     3301 ttccagagtc tcttcggggc gctcgacctc ggcgacggcg ggcgcgtgtt cctgttccgc
     3361 gatgacggcg tcctgcttac cacctatcct ccggaagaga gggcgtacgg acgctcattc
     3421 gcgggcgatg cgctgtttgc aggcgcgccg accagggcgg cggcgtccta cgccgagcgc
     3481 cgtgcaggtc tgctggacgc ggcacctcgc atcgtggctc atgatcacct caagggttat
     3541 ccgctggtgg tggcggtgtc ttccaccgag gacttcattc tcggcgcgtg gcgccgtgac
     3601 gtgtggcgca tcggtgccgg cgcggcggga actgtagcct tcatcggcat cgtgctctat
     3661 tttctgctgc ggcaactgcg cgtgagcgtc ggactggaaa atgagttgcg ggaaaccggc
     3721 gagcggttgc aaggcatcat acgatcggcg atggacgcga tcatcacggt ggacgagcgt
     3781 cagaatatcg tgatgttcaa cgcggctgcc gagaagattt tccgttgctc tggggcggag
     3841 gcgatcggcg gaccgctcga tcgtttcatt cccgagcgct tccgtgcggc gcaccggcgg
     3901 cacgtctggc gcttcggaga gaccggggag accacgcgca tgatgggcgc gcgcctggcg
     3961 cttttcgggt tgcgctccga tggcgaggag tttccgatcg acgcctcgat ctcccagatt
     4021 tcgatcgaag gcaggaagct ctacacggtg atcctgcgcg acatcacccg gcgcaaaatt
     4081 gccgaggatg cattggagcg ctcgtatcgg gagctgcgcg agctttccgc caccatgaac
     4141 gaggtgcgcg aggccgagcg cctgcgaatc gcgcgcgagc tgcacgacga gctggcgcag
     4201 tggctgaccg caatcaagat ggatgtctcg tggctctcgt cgcgattgcc gcgggaacac
     4261 gcgcatctcg tcgacaagac cgagaagatg aaagcggtgg tggacaccac ggtggcggcg
     4321 gtgcggcgca ttgcggcgga cctccgtccg gtcatgctcg acgacctggg actggttgcc
     4381 gcattggagc acttgttgca tgatctgtcg caacgaaccg ggatcatcgt cagcctcgag
     4441 gcggacgccg gagagctgga cctgggcgag ccgcttgcca cttcgctcta caggatggcg
     4501 caggaggcgc tgaccaacgt cgcgcgccac gccgaggcga cgcaggtcca gctcgcgctg
     4561 tgggtcgagg acggccggct cgtgctcacg gtgcgcgaca acggcaaggg aatcgacgcc
     4621 gcagccctgg ggcgcaggaa gtcgtacggc gtgctgggca tccgggagcg cgcccatacg
     4681 ctcggcggaa gcgcgcgcgt cgagcggctc gcgaccggcg gcacgctggt cgagatcgtg
     4741 attccggtca cgcgctaccg cgggcgggag gcggcagatg atacgggtgt tgctggctga
     4801 cgatcacaca atcgtcaggg ccggcctcaa ggagatcctg gccgacaccg gcgacatcac
     4861 ggtggcggcg gaggcgacca acgggcagga agtgatggcg caggtccgcg ggcaggactt
     4921 cgacgtggcg gtgctcgaca tgtccatgcc gggtcgcagc gggatcgagc tgatcaagca
     4981 ggtgaaggac gagaagccga ggttgcccat cctcatcctc agcatgcaca gcgaagaaca
     5041 gtacgcggtg cgcgcgctga aggcgggcgc atcgggttac ctcaccaaag atgcggcagc
     5101 cgatcagtta gtggccgcca tccgccgcat cgctgcgggc ggcgcttacg tgagcccgga
     5161 aacggcggag cggctcgccc tcgatgcggg gtcgcggccg gaagccgcgc cccacacgct
     5221 gctctccgac cgcgaattcc aggtgttcct gatgatcgca cggggcgtgt cggtgagcga
     5281 gatcgcacgc agcctctcgt tgagcgtcaa gaccgtgagc acccacaaga cgcgcatcct
     5341 ggagaaaatg ggtctcgcca accaggccga gctgatccgc tacgccctcg agcaccagct
     5401 ccttgacgaa ccgcccggct cgtcaaggtg acgagtgata agtgataagt gacagg
//
LOCUS       DBNGWZ010000506         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3089929, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000506 DBNGWZ010000000
VERSION     DBNGWZ010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3089929"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="AC16CX_15480"
     CDS             complement(<1..1326)
                     /locus_tag="AC16CX_15480"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3013943.1"
                     /translation="MPKRNVTTMRAAILQRRNRSNAARIHIVAAGGGGMSRRLVTAPF
                     GYNAVTIEACEMTLVARDAGVTEALADWLCSRRWEDLPESVRHKAVDVVYDSVGAMVA
                     CSRLPEVQAIVGFLRKMGGAAECSIIGHATRASPVNAAMANGGMSHGDEADPVHLKSV
                     GGHVASGPVPTALTLGQWTDASGKDVLRAVALGYEVGGRLMTIFYRERDYVARRFYPT
                     AVVATISSAVTAGILLGLERERMQVAMCLAAYQAAGPDNMTKDPAHMGKTFQVAAANR
                     NGVTAALLANDGCHAPLDVLDGSHSLFDAYLGAPDAGAELLKDLGTYYSITDVMHKRY
                     PVGSPNQAYLQGLFGLMEKHAVRPDDITAIEVQIPARGLKRVPTTRHASISALSVCAI
                     AAAHGKLDFYRLHDPAGAMDAAARDMQERVKFVGREDWTDLQAGHRAIVT"
     gene            1340..>2675
                     /gene="purB"
                     /locus_tag="AC16CX_15485"
     CDS             1340..>2675
                     /gene="purB"
                     /locus_tag="AC16CX_15485"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3013944.1"
                     /translation="MALSPLTALSPLDGRYHSKLAALRDTFSELALIRHRVKIEIEWL
                     KALAASRAIKEVPPFSPPTLAQLDEVAAKFSEADGESVKTTEAKINHDVKAIEYFLRE
                     KLSGNKEVTKVAEFIHFACTSEDINNLAYALMLKRARDEVILPALDRVIEKLAGLAHE
                     LADAAMLAYTHGQPASPTTLGKELANVAHRLVRGRERIAGVKLTGKFNGAVGNYNAHL
                     AAYPGVDWEKLARSLVEGFGLEFNPYTIQIEPHDSIAELFDASSRVNTMLIDFDRDVW
                     GYISLGYFRQKVKEGEVGSSVMPHKVNPIDFENSEGNLGVANALLRHMSEKLPISRWQ
                     RDLTDSTVLRNMGVALGHALLGYGACLRGLNKLEADRNRMKEDLDANWEVLAEPIQTV
                     MRRYGITGGYEKLKELTRGKGGVTRDTVQAFIKGLKIPEAEKKRLLALTPATY"
BASE COUNT          481 a          900 c          860 g          434 t
ORIGIN      
        1 ggtcacgatc gcgcggtggc cggcttgcag atccgtccag tcttcgcggc cgacgaactt
       61 gacgcgctcc tgcatgtcgc gcgccgcggc atccatcgcc ccggcgggat cgtgcagccg
      121 gtagaaatcg agcttgccat gcgccgccgc gatggcacat accgacagcg ccgaaatcga
      181 ggcgtggcgc gtggtcggaa cccgtttcag gccgcgggcc ggtatctgca cctcgatcgc
      241 ggtgatatcg tcgggccgca ccgcgtgttt ctccatcagg ccgaagaggc cctgcaagta
      301 cgcctggttc gggctgccca ccgggtagcg cttgtgcatg acatcggtga tcgaataata
      361 cgtcccgagg tccttcagca actccgcgcc ggcatccggg gcgcccagat aggcatcgaa
      421 caggctgtgc gagccgtcga gcacgtcgag cggagcgtgg cagccgtcgt ttgccagcag
      481 cgctgcggtg acgccgttgc ggttggctgc cgcaacctgg aacgtcttgc ccatgtgggc
      541 ggggtccttg gtcatgttgt cggggccggc tgcctggtat gccgcgaggc acatggcgac
      601 ctgcatccgc tcgcgttcga gaccgagcag tatgccggcc gtcacagcgg aactgatcgt
      661 cgcaacgacc gccgtgggat agaagcggcg ggctacgtag tcgcgctcgc ggtagaagat
      721 ggtcatgagc cggcctccta cctcgtatcc gagcgcaacc gcgcgcagca catccttgcc
      781 gcttgcgtcc gtccactggc cgagcgtgag cgcagtcggt accggccccg aggcgacgtg
      841 accgccgacc gatttcaggt gcaccgggtc ggcttcatcg ccgtgcgaca tgccgccgtt
      901 cgccatcgcc gcgttgacgg ggctggcgcg ggttgcgtga ccgatgatcg agcactccgc
      961 ggcgccgccc atttttcgca ggaatccgac gatcgcctga acctcgggca atcgcgagca
     1021 agcgaccatc gccccgaccg agtcgtacac gacgtccacc gccttgtggc gcacgctctc
     1081 cggcaggtcc tcccagcggc ggctgcacaa ccagtccgcg agcgcttcgg tgacgcccgc
     1141 atcgcgtgcg acgagggtca tttcgcaagc ctcaatagtg acggcattgt agccgaaggg
     1201 tgccgtcacc aaccgccggg acatgccgcc cccgcccgcg gccacgatgt ggatgcgcgc
     1261 cgcgttcgag cggtttctcc gctggaggat ggccgcccgc atcgtggtaa catttcgctt
     1321 gggcatgcat tcatccgtca tggcgctctc ccctctcacc gccctttccc cactcgacgg
     1381 ccgctatcac agcaagctcg ccgcgctgcg cgacactttc agcgagcttg cgctgatccg
     1441 gcaccgcgtg aagatcgaga tcgagtggct gaaggcgctc gccgccagcc gcgcgatcaa
     1501 ggaagtgccg ccgttttccc cgcccacgct ggcgcagctc gacgaagtgg ccgcgaaatt
     1561 ctccgaggcc gacggcgagt cggtgaagac gaccgaggcg aaaatcaatc acgacgtcaa
     1621 ggcgatcgag tactttctgc gcgagaagct ctcgggcaac aaggaagtga cgaaggtcgc
     1681 ggagttcatc cacttcgcct gcacctccga ggacatcaac aatctcgcct acgcgttgat
     1741 gctgaagcgc gcccgcgacg aagtgatcct gcccgcgctc gatcgcgtga tcgagaagct
     1801 cgccggcctc gcccacgagc tcgccgacgc cgcgatgctt gcctacaccc acggccagcc
     1861 ggcaagcccg accacgctgg gcaaggagct cgccaacgtc gcccaccggc tggtgcgggg
     1921 acgcgagcgc atcgcgggcg tgaagctgac aggcaagttc aacggcgcgg tcggcaacta
     1981 caacgcgcac ctcgccgcct atcccggcgt ggactgggag aagctcgccc gcagcctggt
     2041 cgagggcttc ggcctcgaat tcaacccgta cacgatacaa atcgagccgc acgattcgat
     2101 cgccgagctg ttcgacgcca gctcgcgcgt gaacaccatg ctgatcgatt tcgaccgcga
     2161 cgtctggggt tacatctcgc tcggctactt caggcagaaa gtgaaggaag gcgaggtcgg
     2221 ctcatcggtc atgccgcaca aggtgaaccc gatcgatttc gagaactcgg aaggcaatct
     2281 cggcgtcgcc aacgcgctct tgcggcacat gagcgagaag ctgccgatct cccgctggca
     2341 gcgcgaccta accgactcca ccgtgctgcg caacatgggg gtcgcgctgg gacacgctct
     2401 gctcggctac ggcgcctgcc tgagggggct caacaagctg gaagccgatc gcaaccgcat
     2461 gaaagaggac ctcgacgcca actgggaggt gctcgccgag ccgatccaga ccgtgatgcg
     2521 gcgctacggg atcaccggcg gctacgagaa gttgaaggag ctgacgcgtg gcaagggagg
     2581 cgtcacgcgt gacacggtgc aggccttcat caagggcctc aagataccgg aagcggagaa
     2641 gaagcggctg cttgcgctca cgcccgcgac ctaca
//
LOCUS       DBNGWZ010000507         7823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2585464, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000507 DBNGWZ010000000
VERSION     DBNGWZ010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2585464"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="AC16CX_15490"
     CDS             <1..943
                     /locus_tag="AC16CX_15490"
                     /inference="COORDINATES: protein motif:HMM:NF020660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR3013945.1"
                     /translation="NDAYWHGLFGGIYLPHLRRAVYRELLEVERGLDALSPRPPAACC
                     DLDHDGSEELFLCRLRLQAVLALDGRAAIREFDSYALRHNFSDVLRRTPEHYYCKMRA
                     RREEGEPATGIASAHDRVRFKHEIGPADLVADSRPRAQFVDDWTADGGGLVDLAGYEL
                     QFVDAAGRAAVFSSPLGDGTVTKRVTLEDDRLVVNYAIRGQRGGVFGTELNLALPSCD
                     GFGGRYVLADGTMPCGFGQELDIARLETIVLDDRELQGRLSLMVRPAAALRARPHYTV
                     SQSEAGFERIMQGAVLALKWRCDPPAEITVVLEVRAG"
     gene            1066..3612
                     /gene="glgP"
                     /locus_tag="AC16CX_15495"
     CDS             1066..3612
                     /gene="glgP"
                     /locus_tag="AC16CX_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR3013946.1"
                     /translation="MPGTTYHIEVNPQIPQRLLRLEELANNLWYSWDRPTRALFSRLH
                     PALWHAVGHSPKAFLKRVDQQRLVDAADDQVFLGAYNYVLSAYDTYHNEPTRATHGLR
                     QSDMVAYFCAEFGFHESLPIYSGGLGILAGDHCKAASDLRLPFVGVGLLYRQGYFHQT
                     IDNDGNQHAVYADSDFEDLPISPAVRENGSELRVTVELPGRSVAVKVWRARVGHVMLY
                     LLDTDLEENAAHDRDIAHRLYGGDRTTRIEQEIILGIAGVRALQEAGVKPTVWHINEG
                     HPAFLVLERIRMLVKQGLRFDAALEAAAANTVFTTHTAVPAGHDHFTTEIVLHHLESW
                     CRDVGVEPATLFALGKAGDSADFNMTTLAIRGSRHHNGVSRIHCDVSARICAALWPEI
                     EPEENPVTYVTNGIHVPTFLAEDWYELFDRHLGGGWSQRQTDQACWDGVHSIPDPAFW
                     GIRQALKSEMLYLVRHRIREQHARNHGSESHLDRILRLADPANPNVLTIGFGRRFATY
                     KRATLLFEDLEWLREIVCDPQRPVLLLFAGKAHPADQPGQDLIRRIAEIARMREFESH
                     ILLIEGYDLQLARRLVSGVDVWLNNPVYPLEACGTSGMKSAFNGVINLSVLDGWWGEG
                     YDGKNGWAIKPASRSLDEARRNQEESRTLYEILQDSVVPLYYNRGPTGFSPEWVAMAK
                     RSISSLLPCFNSTRMLNEYVTKFYVPASSQGRRYAQDDYGGARALAAWKAKVRAAWKQ
                     VSARRLDLPLRRIHFSETLKVEVAVNLDGLGPEDVVVELLFGRPGRVAEETVHSHQLK
                     HAGPVPGGTEHLFALELTPELCGKIEYRIRVYPYHELLTHRFEMGMTKWV"
     gene            3684..5162
                     /gene="glgA"
                     /locus_tag="AC16CX_15500"
     CDS             3684..5162
                     /gene="glgA"
                     /locus_tag="AC16CX_15500"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR3013947.1"
                     /translation="MPSDPPLRVLFATPELAPWVKSGGLGEVSATLPAALRELGADVR
                     VLVPGYPAIVATHPDARVAAALSNPGGALAGARLLAVEKPRQVPLLIIDCPGYYRREG
                     TAYQDERRHDWRDNHLRFGLLSRVAALLAGEASPLAWRPHILNCHDWPAGLAPAYLHF
                     AHGARAVSVMTVHNLAFQGIFPAAALQELGLPQQAFHVGGVEFHGQVSFLKAGLAYAE
                     QLTTVSPRYAREIRTDELGCGLGGLLRRRRDRLVGILNGIDTSVWDPATDPFLAAPYD
                     FSRIVDKALNKAALQRELGLRVDARAPLLAVVSRMTHQKGVDLVAAIAPEIAALPAQL
                     AVLGTGEPALERDFTALARRFPGAIAAIIRFDEALAHRIEAGADVFVMPSRFEPCGLN
                     QMYSLRYGTPPVVRATGGLADTVVDATPANLAAGTATGFTFDAAEPPALLEALARAAR
                     AWREEGTWRALQRVGMAQDHSWSRSARRYLDLFQALARMTRT"
     gene            5518..7338
                     /gene="ftsH"
                     /locus_tag="AC16CX_15505"
     CDS             5518..7338
                     /gene="ftsH"
                     /locus_tag="AC16CX_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3013948.1"
                     /translation="MDRPTRINLAYAVLAVLAILWMQSVWVKAQQVERLSYSEFQAQL
                     KSGNVKEITVYENRIEGVFNRPMADGHTRFVTVRVEPELAKDLEQYNVRFSGAVESTF
                     LRDILSWVVPTLLFFALWMFLVRRIAQGQGMGGGFLTIGKSKAKVYMEKDTKVTFDDV
                     AGVDEAKEELKEVVNFLKNPQSYGRLGARIPKGVLLVGPPGTGKTLLARAVAGEAGVR
                     FFSISGSEFVEMFVGVGAARVRDLFEQARQQAPAIIFIDELDALGRARTAFPLGGHDE
                     KEHTLNQLLSEMDGFDPSAGLVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKKGRV
                     QILKTHVRKVRLAADVDLDKVAGLTVGFSGADLANLVNEAALLATRRGGDAVTADDFT
                     RAVERIVAGLEKKNRVLNARERQVVAYHEMGHALVALNLPGVDPVHKISIIPRGIGAL
                     GYTIQRPTEDRYLMTRGELENKMAVLLGGRAAEHIVFGHLSTGAADDLAKVTDIARNM
                     VMRYGMVETLGHVVYEAERPTFLGVPEGAMRGREFSEETAREIDRAVREIVKIAFDRA
                     VGILNERRDLLERGAQALLQKETLVEEDLAALRRELQAAA"
     gene            complement(7420..>7823)
                     /locus_tag="AC16CX_15510"
     CDS             complement(7420..>7823)
                     /locus_tag="AC16CX_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR3013949.1"
                     /translation="YHVRIDVTVPDGELVASREPDEHHAYTDVYVAIRDAFDTMRRRL
                     EDYARRRRREVKAHEAPPHGRIVELHPEEGYGRIESSDGRLVYFHRNSVLEADFDQLK
                     IGAEVRFAEEMGERGPQATTVHPVGKHHVVG"
BASE COUNT         1263 a         2586 c         2710 g         1264 t
ORIGIN      
        1 caacgacgct tactggcacg gcctgttcgg tggaatctac ctgccgcacc tgcggcgcgc
       61 cgtgtaccgg gaactgctcg aggtcgagcg cgggctcgac gccctctcgc cgcggccccc
      121 ggccgcctgc tgcgatctcg accacgacgg cagcgaggag ctgttcctgt gccggctgcg
      181 cctgcaggcg gtcctggcgc tcgacggacg cgcggcgatc cgggagttcg acagctacgc
      241 gctcaggcac aatttcagcg acgtgctgcg ccggacgccg gagcactact actgcaagat
      301 gcgcgcgcgg cgggaggagg gcgagccggc aaccggcatc gcgtcggccc acgaccgcgt
      361 gcgcttcaag cacgagatcg gtcccgcgga cctcgttgcc gactcccggc cgcgcgccca
      421 gtttgtcgat gactggacgg cggatggggg gggcctcgtc gatctggcgg gctacgaact
      481 gcagttcgtg gacgccgccg ggcgcgcagc ggttttttct tctccgctcg gtgacggcac
      541 ggtcacgaag cgcgtcacac tcgaggacga caggctcgtc gtcaactacg cgattcgggg
      601 gcagcgcggg ggcgtgttcg gcaccgagct caatctcgca ttgccgagct gcgacggttt
      661 cggggggcgt tacgttctcg cggacggcac gatgccctgc ggcttcggac aggagctgga
      721 catcgcacgg ctcgagacga tcgtgctcga cgaccgcgag cttcaaggcc gcctctcgct
      781 catggtgaga ccggcggcgg cattgcgcgc gcgtccgcat tacaccgttt cgcagtccga
      841 ggcggggttc gagcgtatca tgcagggcgc ggtgctcgcg ctgaagtgga gatgcgaccc
      901 accggcggag atcacggtgg tgctcgaagt gagagccggg tgacgcgctt gccgcgccgc
      961 agcccggcgt ttgaccctca tgccgctgga atggcacatt tcaggtttgc aggcgcggcg
     1021 ggagcgggac gcccactccg gcgctgatcg ggacgtggag aggtaatgcc gggaacaacc
     1081 tatcacatcg aagtcaatcc gcagataccg cagcggctgc tgcggctgga ggagctggcc
     1141 aacaatctct ggtacagctg ggaccggccg acgcgggcgc tgttctcgcg cctgcatccc
     1201 gcgttgtggc acgcggtcgg ccacagcccg aaggcgttcc tgaagcgcgt cgatcaacag
     1261 cggctggtgg acgccgcgga cgaccaggtg tttctgggcg cctacaacta cgtgctctcg
     1321 gcctacgaca cgtatcacaa cgagccgacg cgcgcgaccc acggtctgcg gcagagcgac
     1381 atggtggcgt atttttgcgc cgagttcggc tttcacgaga gcctgccgat ctattccggc
     1441 gggctcggca tcctcgccgg cgaccactgc aaggcggcga gcgacctgcg gctgccgttt
     1501 gtcggcgtcg ggctgctcta ccggcagggc tacttccacc agaccatcga caacgacggc
     1561 aatcagcacg cggtgtatgc ggattccgac ttcgaggacc tgccgatttc gcccgccgtg
     1621 cgcgagaacg gctccgagct gcgcgtcacc gtggagctgc ccgggcgcag cgtggcggtc
     1681 aaggtgtggc gggcgcgcgt cgggcacgtc atgctctatc tgctcgatac cgacctcgag
     1741 gagaacgccg cgcacgaccg cgacatcgcg caccgtctct acggcgggga ccgcaccacg
     1801 cgcatcgagc aggaaatcat cctcggcatc gccggcgtgc gggcgctgca ggaagcgggc
     1861 gtcaagccca ccgtgtggca catcaacgaa gggcatccgg cgtttctcgt gctcgagcgc
     1921 atacggatgc tggtcaagca ggggctgcgg ttcgacgcgg cgctggaagc ggcggcggcg
     1981 aacacggtgt tcacgacgca caccgcggtc ccggccgggc acgaccattt cacgaccgag
     2041 atcgtgctcc atcacctcga gtcctggtgc cgcgacgtgg gcgtcgagcc ggcgacgctg
     2101 ttcgcgctcg gcaaggcggg cgacagcgcc gatttcaaca tgaccacgct cgcgatccgc
     2161 ggctcgcgcc atcacaacgg ggtgagccgc atacactgcg acgtttcggc gcggatctgc
     2221 gccgcgctgt ggccggagat cgagccggag gaaaacccgg tcacctacgt cacgaacggc
     2281 atccacgtgc cgacgttcct ggcggaggac tggtacgagc tgttcgaccg ccatctcggc
     2341 ggcggctgga gccagcgcca gaccgaccag gcatgctggg acggcgtcca ttcgatcccc
     2401 gacccggcgt tctggggcat ccgccaggcg ctcaagtcgg agatgctgta cctggtgcgg
     2461 catcgtatcc gcgagcagca tgcccgcaac cacggctccg aatcgcacct cgaccgcatc
     2521 ctgcggctcg ccgatcccgc caatccgaac gtgctcacga tcggcttcgg gcggcgcttc
     2581 gccacctaca agcgcgcgac gctcctgttc gaggatctcg agtggctgcg cgagatcgtg
     2641 tgcgacccgc agcggccggt gctgctgctg ttcgcgggca aggcgcaccc ggcggaccag
     2701 ccggggcagg acctgatacg gcgcatcgcg gagatcgcgc gcatgcgcga gttcgaaagc
     2761 cacatcctgc tgatcgaggg ctacgatctg cagctcgcgc ggcggctggt gtccggcgtg
     2821 gacgtgtggc tcaacaaccc ggtgtatccg ctggaagcct gcggcacttc gggcatgaag
     2881 tcggccttca acggcgtcat caacctgagc gtgctcgacg gctggtgggg cgagggctac
     2941 gacggtaaga acggctgggc aatcaagccg gcctcgcgct cgctcgacga ggcgcgccgc
     3001 aatcaggagg agtcgcgcac gctgtacgag atcctgcagg acagcgtggt cccgctctac
     3061 tacaaccgcg gaccgaccgg tttttcgccc gagtgggtcg cgatggccaa gcgttcgatc
     3121 tcctcgctgc tgccgtgctt caactccacg cgcatgctga acgagtacgt gaccaagttc
     3181 tacgttcccg ccagcagcca gggccgccgc tatgcccagg acgactacgg cggggcgcgg
     3241 gcgctggcgg cctggaaggc gaaggttcgc gccgcgtgga agcaggtgag cgcgcgccgg
     3301 ctcgacctcc cgctgcggcg catccacttc agcgaaacgc tgaaggtcga ggttgctgtc
     3361 aacctggacg ggctcggccc cgaggatgtc gtcgtggagc ttctcttcgg caggccgggc
     3421 agggtcgcgg aggagaccgt acattcgcac cagttgaagc acgcgggtcc cgtgccgggc
     3481 ggcacggagc atctgttcgc gctcgagctg acgcctgagc tgtgcggcaa gatcgagtac
     3541 cgcatccgcg tctatcccta ccacgagttg ctgacccatc gcttcgagat gggcatgacg
     3601 aagtgggtgt gagggcgtct gccatggcgc tgctccggcc gcggcggacg acgcgcgcct
     3661 gatcgatcct gagctctttt ttcatgccaa gcgatccgcc gttgcgcgtg ctgttcgcga
     3721 cgccggaact ggcgccatgg gtcaaaagcg gcgggctcgg tgaagtttcg gcgactctgc
     3781 ccgccgccct ccgcgagctg ggcgccgacg tgcgcgtgct ggtgccgggt tacccggcga
     3841 tcgtagcgac gcaccctgat gcccgcgtcg cggcggcgct ctcgaatccc ggcggcgcgc
     3901 tcgcgggcgc gcggctgctt gccgtggaaa aaccgcggca ggtgccactg ctgatcatcg
     3961 actgccccgg ctactaccgc agggaaggca cggcatacca ggatgaacgc cgtcatgact
     4021 ggcgcgacaa ccacctgcgg ttcggattgc tgtcgcgggt ggcggcgctc ctcgcggggg
     4081 aggcgagccc cctcgcctgg cgccctcata tcctgaactg ccacgactgg ccggcggggc
     4141 ttgcgccggc ttacctgcac ttcgcgcacg gggcgagggc ggtctcggtc atgaccgtgc
     4201 acaacctcgc tttccaggga attttccccg ccgccgctct gcaggaattg ggcctgccgc
     4261 agcaggcgtt tcatgtcggc ggcgtggaat tccacggcca ggtgtcgttt ctcaaggcgg
     4321 gactcgccta cgccgagcag ttgacgaccg tgagtccgcg ctacgcgcgc gagatccgga
     4381 ccgacgagct cggctgcggc ctcggcggcc tgctgcgccg tcggcgcgac cggctcgtcg
     4441 gcatcctgaa cggcatcgac acgagcgtct gggacccggc gacggacccg ttcctggccg
     4501 ctccctacga tttttcccgc atcgtggaca aggcgctcaa caaggccgcg ctgcagcgcg
     4561 agctcgggtt gcgcgtcgat gcgcgggcgc cgttgctcgc cgttgtcagc cgcatgacgc
     4621 accagaaagg cgtcgatctc gtcgcagcga tcgcccccga gattgccgcg cttccggcgc
     4681 agctcgcggt gctcgggacc ggcgagccgg cgctcgagcg cgacttcacg gctctcgcgc
     4741 ggcgcttccc gggggcgatc gcggcgatca tccggttcga cgaggcgctc gcccaccgca
     4801 tcgaggcggg cgcggatgtc ttcgtgatgc cgtcacggtt cgagccgtgc gggctcaacc
     4861 agatgtacag cttgcgttac ggcacgccgc ccgtcgtgcg cgcgaccggc ggcctggcgg
     4921 acaccgtcgt cgacgccacg cccgctaatc tggcggccgg caccgcaacg ggcttcacgt
     4981 tcgacgccgc ggagccgcca gcgctgctcg aggcgctggc gcgcgcggcc cgcgcctggc
     5041 gggaagaggg cacatggagg gcgttgcagc gggtgggcat ggcgcaggac cacagctgga
     5101 gccgcagcgc ccggcgttac ctggacttgt ttcaggctct agcccgcatg actcgcacgt
     5161 aacagggttg gagagtatcc gagacgggaa cgcttcggcc ggcgtcggag catgcgggct
     5221 gatgaattct tgaaaaaaga accaaaacgc gcgacagctc ctgcttggcg ctgtttgccg
     5281 gaatttcaaa ttccattgtt caaaaaatcg tccgcccgcg tgccccaacc cccattcggc
     5341 gctctggcct ttaaaatccg tcgtctgctg ctactcatga aacacgcggg cgaagcattt
     5401 gatctgaatc aaccccggta ctggccgcag ggatagagta gtttccggtc cggaagccgg
     5461 ggcttggcgt gaccggaacc ggacagaacc ccgcgatcac gattgcggaa ggaaccgatg
     5521 gacaggccga cccggatcaa ccttgcttac gccgtactgg ccgtgctcgc catcctgtgg
     5581 atgcagagcg tctgggtgaa ggcgcagcag gtcgagcggc tctcgtacag cgagttccag
     5641 gcccaactca agagcgggaa cgtcaaggaa atcacggtct acgaaaaccg catcgagggc
     5701 gttttcaatc gtccgatggc agacggccat acccggttcg ttacggtccg cgtggaaccc
     5761 gagcttgcga aggacctgga gcagtacaac gtgaggttca gcggcgcggt cgaaagcacg
     5821 ttcctgcgcg acattctctc gtgggtcgtc ccgacgcttc tgttcttcgc gctgtggatg
     5881 ttcctggtgc gccgcatcgc ccagggccag gggatgggcg gcggctttct caccatcggc
     5941 aagagcaagg cgaaggtgta catggaaaag gacaccaagg tcaccttcga cgacgtcgcc
     6001 ggcgtggacg aagcgaagga ggaattgaag gaggtcgtca acttcctcaa gaatccgcag
     6061 agctacggac gcctcggcgc gcgcattccc aagggggtgc tgctggtcgg cccgcccggc
     6121 accggaaaaa cgctgctggc acgggccgtg gccggcgaag cgggcgtgcg gttcttctcc
     6181 atcagcggct ccgagtttgt cgagatgttc gtgggcgtgg gcgctgcgcg ggtgagggac
     6241 ctgttcgagc aggcgcgcca gcaggcgccg gccatcatct tcatcgacga gctcgacgcc
     6301 ctcggccgcg cgcggaccgc gtttccgctg ggcggacacg acgaaaagga gcacacgctc
     6361 aaccagctgc tctcggaaat ggacggcttc gatcccagcg cgggcctcgt cctgcttgcc
     6421 gccaccaacc gcccggaaat cctcgatccc gcactgctgc gggcaggccg cttcgaccgg
     6481 caggtgctgg tggaccggcc cgacaagaag gggcgcgtgc agatcctgaa gacgcacgtg
     6541 cgcaaggtgc gccttgcggc ggacgtggat ctggacaagg tcgccggatt gacggttggc
     6601 ttctcggggg cggatctcgc gaatctcgtc aacgaggcgg ccctgctcgc cacgcggcgc
     6661 ggcggcgacg cggtgacggc ggatgacttc acacgggcgg tcgagcggat cgtcgccggc
     6721 ctcgagaaga agaaccgtgt gctcaatgcg cgcgagcgcc aggtcgtcgc ctaccacgag
     6781 atgggtcatg cgctggtcgc gctcaacctg cccggcgtgg atcccgtcca caagatctcg
     6841 atcattccgc gcggaatcgg cgcgctgggc tacaccatcc agcggccgac cgaggaccgc
     6901 tatctcatga cccgcggcga gctcgagaac aagatggccg tgctgctggg cgggcgggcg
     6961 gccgagcata tcgtattcgg ccatctctcc accggcgcgg cggacgacct cgccaaggtg
     7021 accgacatcg cgcgcaacat ggtgatgcgc tacggcatgg tcgagacgct cggccatgtc
     7081 gtatacgaag ccgagcgccc cacgtttctg ggcgtgccgg agggcgccat gcgcggacgc
     7141 gagttcagcg aggaaaccgc gcgcgaaatc gaccgcgccg tgcgggagat cgtgaaaatc
     7201 gccttcgacc gcgccgtggg gatcctcaac gagcgccgcg acttgctcga gcgcggggcc
     7261 caggcgctgc tgcagaagga aaccctcgtt gaagaggacc tggcggccct gcggcgtgag
     7321 ctgcaggcgg cggcctgacc gcggcgcggc cgcccgaccg ggaatgcgcc cgccgcaggc
     7381 tgccggagag gtagcaggcg cgctgacttc agcagcccgc taaccgacga cgtgatgctt
     7441 gccgaccgga tggaccgtgg tcgcctgcgg gccgcgctcg cccatctctt cggcgaagcg
     7501 cacctcggcg ccgatcttca gctggtcgaa atccgcctcc agcacgctgt tgcggtggaa
     7561 atagacgagc cgcccgtcgg atgactcgat ccggccgtag ccttcctccg gatggagctc
     7621 gacgatgcgg ccgtggggcg gcgcctcgtg cgccttgacc tcccggcggc gccggcgggc
     7681 gtagtcttcg agccggcggc gcatggtgtc gaacgcgtcg cggatggcga cgtagacgtc
     7741 ggtgtacgcg tgatgctcgt ccggctcccg gctcgcgacg agctcgccat cgggcacggt
     7801 gacatcgatg cggacgtggt agt
//
LOCUS       DBNGWZ010000508         1511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_694844, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000508 DBNGWZ010000000
VERSION     DBNGWZ010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_694844"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="AC16CX_15515"
     CDS             complement(<1..120)
                     /locus_tag="AC16CX_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR3013950.1"
                     /translation="MIGLLQRVDSAKVTVDGAAIAEIGRGLVVLVGVEAGDGRP"
     gene            complement(117..>1511)
                     /locus_tag="AC16CX_15520"
     CDS             complement(117..>1511)
                     /locus_tag="AC16CX_15520"
                     /inference="COORDINATES: protein motif:HMM:NF039580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR3013951.1"
                     /translation="IWLFIILLLLAVWAALDRLLVPGVRWYFRRKVNRVIREISTRLN
                     IELPQFKLARRQVLIDRLFHDPKVQAAVERECREKGLPRDVVAARVDRYAREIVPAFN
                     AYLYFSVGYWLGKRLARTLYRVRLGYADTQALAAVNPKSSIVFVMNHRSNMDYVLVAF
                     LAAERVALSYAVGEWARIWPLQQLIRSMGAYFVRRNSGDALYRLVLQRYVQMAVEAGV
                     PQAVYPEGGLSTDGRLRPPRLGLLDYMLKTFDPGAERDLVFIPVGINYDRVLEDRMLL
                     MKLDAAAPRRGFLYTAARTAAFAAGNLWLMVTGRWYRFGYACVNFGRPISMREYVARH
                     GVDFRWLDEEARHREVGRIGAQIMDAVARIVPVLPVSLVATVFLRSHEAGLSELELKS
                     AAYDLILRLEKSDAHIYVPRGDLDYAIGVGLRMLLLRRIVAEQDGLYRANPAELALLA
                     YYANAIEPLLASPE"
BASE COUNT          286 a          541 c          463 g          221 t
ORIGIN      
        1 tggccttccg tctccagcct ccaccccgac caggaccacg agcccgcgcc cgatctcggc
       61 gatggcggcg ccgtccaccg tcacctttgc cgaatccacc ctctgcaaca gtccgatcat
      121 tcgggactcg caagcagcgg ctcgatcgcg ttcgcgtagt acgcgagcag cgcaagctcg
      181 gcgggattgg cacggtagag tccatcctgc tccgcgacaa tgcggcgcag cagcagcatg
      241 cgcaggccga ccccgatggc gtaatcgaga tcgccgcgcg gaacgtagat gtgcgcgtcg
      301 ctcttctcca gccgcagaat gaggtcgtag gcggccgatt tgagctcgag ctcggacagg
      361 ccggcctcgt ggctgcgcag gaacacggtg gcgacgagcg aaaccggcaa taccggcacg
      421 atgcgcgcga ccgcatccat gatttgcgcg ccaatccgcc cgacctcgcg gtggcgcgcc
      481 tcctcgtcga gccacctgaa atccacgccg tgccgcgcca cgtactcgcg catcgagatc
      541 ggccgcccga aattgacgca ggcatagccg aagcggtacc aacgaccggt caccatcagc
      601 cacaggttgc cggcggcgaa cgcggccgtc ctggccgccg tataaagaaa gccgcgccgc
      661 ggcgcagccg catcgagttt catcaacagc atgcggtcct cgagcacgcg gtcgtagttg
      721 atgcccaccg gtatgaacac gagatcgcgc tcggccccgg gatcgaaggt cttcagcatg
      781 taatcgagga gcccgagcct gggaggccgc agtctcccgt ccgtggacag gccgccctcg
      841 ggatacaccg cctgcggcac gcccgcttcg accgccatct gcacgtagcg ctgcagcacc
      901 aggcggtaca atgcgtcgcc cgagttgcgc cgcacgaagt aggctcccat cgagcggatc
      961 agctgctgca gcggccagat cctcgcccat tccccgacgg cgtatgaaag cgccacgcgc
     1021 tcggcggcga gaaaagccac cagcacgtag tccatgttgc tgcggtggtt catcacgaac
     1081 acgatgctcg acttcggatt gaccgccgcg agcgcctgcg tgtcggcgta gcccagccgc
     1141 acgcgataaa gcgtcctggc cagcctcttc cccagccagt aaccgacgct gaaataaaga
     1201 taggcgttga acgccggcac gatctcgcgc gcgtagcggt caacacgcgc cgcgacgacg
     1261 tcccgcggca gccccttctc gcggcactcc cgctcgacgg cggcctgcac cttcgggtcg
     1321 tggaacagac ggtcgatcag cacctggcgc cgcgccagct tgaactgcgg cagctcgatg
     1381 ttgagccggg tgctgatctc gcggatgacg cggttcacct tgcggcggaa ataccaccgc
     1441 acgcccggca ccagcagccg gtcgagcgcc gcccacaccg ccaacagcag cagaatgatg
     1501 aacagccaga t
//
LOCUS       DBNGWZ010000509         9278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1955582, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000509 DBNGWZ010000000
VERSION     DBNGWZ010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1955582"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="AC16CX_15525"
     CDS             <1..507
                     /locus_tag="AC16CX_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HIR3013952.1"
                     /translation="HKRYSGFDALKLYCHRVAGVVGLLSAEIFGYRDRRTLDYAADLG
                     LAFQLTNIIRDVGEDARRNRVYLPLDEIGRYGVSESDIVQARETDNFRRLMEFQIERA
                     LGYYREAFAKLPAMDRRTQRAGLVMAAIYQAVLDEIRRDGCRVLTRRVTLTPIRKLWI
                     AWKTWIRG"
     gene            670..1425
                     /locus_tag="AC16CX_15530"
     CDS             670..1425
                     /locus_tag="AC16CX_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3013953.1"
                     /translation="MDLQLKGKTALVTGASMGIGRAIAKGLAVEGVKACIAARRKPLL
                     AKLAKEIAAAGGPEPFVVEVDILRQGAPEKLAQAAQRALGGIDILVNCAGGSKPPVPI
                     DAPEAEWDEAMTLNFTRLRQLTHAVLPVMIRRKWGRVINISGKSEPERLIAAHAAKAA
                     VHAWSKGLSREVGRYGITVNCIQPGRILSEQILRKYSPEYRKEQSEKEIPVGRYGEPE
                     ELACLAVFLASPVACYITGAVMPVDGGLRRYAF"
     gene            1556..2677
                     /locus_tag="AC16CX_15535"
     CDS             1556..2677
                     /locus_tag="AC16CX_15535"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HIR3013954.1"
                     /translation="MQTKIPCVVMRGGTSRGPFFHASDLPADAPTRDAVLLAVMGSPH
                     EYQVDGIGGAVSVTSKVAIISKSKHPGADVDYLFAQVLINEKLVDTKPNCGNMLVAVA
                     PFAIEEGLVPAKNGETTVRIFNVNTQSLVESIVQTPGGSVTYAGDSAIDGVPGTAAPV
                     KLNFKSAIGSVTGKMLPSGKPLDVIDGVEASCVDVAMPVVLMRAEAFGKTGHEAAVKL
                     DADLELLRRMETIRRKAGALMGMGDVSKLVVPKMALLSKPRRGGAIASRFFVPDACHK
                     AHPVTGTVCIASACAISGTVASQISPLPPAPQGMIRIEHPSGMIIIDLDVDFTGGKQE
                     LRRAALVRTARRIFEGYVNVPEQVWSGDTRAAKPATAAV"
     gene            2700..3989
                     /gene="hpnE"
                     /locus_tag="AC16CX_15540"
     CDS             2700..3989
                     /gene="hpnE"
                     /locus_tag="AC16CX_15540"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HIR3013955.1"
                     /translation="MNVAVIGGGYAGMAAAVTLAERRVPVTVFEAAKTLGGRARRIDH
                     RDVALDNGLHILIGAYSETLRLMRQVGADPERLLLRLPLTWRIHERFSLKAVSLPAPL
                     HLLGGLATARGVPLAERLAAARFLRRMRRQGYALPQDTTVASLLESEAQGGRMTRLLW
                     RPLCVSALNTPSEAASAQVFLNVLRDSLNADRAASDLLLPRVDLSRLFPEPAADYVKA
                     NGGTTLIGQRVTEIEESSIGFTVRCAGVERSFTHVVCALPPYHAGAFLIGISALAEAA
                     EIIEGLTYQPIYSVYLGYPREVRLPAPMLGFESALLQWAFDRGALGGQHGVIGAVISA
                     EGPHQDLAQDELGRLVHQELQQQLGILSEPLWCRVIAEKRATIACTPGLKRPESRTPL
                     RDFLLAGDYVASDHPATIESAVRSGVNAARMILGSKA"
     gene            4167..5798
                     /locus_tag="AC16CX_15545"
     CDS             4167..5798
                     /locus_tag="AC16CX_15545"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR3013956.1"
                     /translation="MQYDVVVVGAGPAGLAAAIRLKQLAAQHQHEISVCVLEKGSEVG
                     AHILSGAVIDPRALNELLPDWQRLNAPLNAPVTEDRFLFLTESASFKVPDIMLPACFR
                     NHGMYVVSLGNVCRWLAQQAEGLGVEIFAGFAAAEVLHDRNGAVLGVATGDMGIGRDG
                     KPTANHQPGMELRARYTLFAEGCRGHLGKRLQDRFNLRAGADPQVYGIGLKELWEVRP
                     ERHRQGLVIHTAGWPLATDTYGGSFLYHLENNQVSIGFVVGLAYSNPYLSPYEEFQRF
                     KTHPAIRPFLEEGKRIAYGARAISAGGLQSLPRLVFPGGCLIGDDAGFLNASRIKGSH
                     AAMKSGMLAAEAAFTALRAGRSHDELGAYPEAFRASWLHDELHRARNFKPWMSKGLVL
                     GSLMVGVDQVVFQGRAPWTLHHAHADHETLRTKSEAEPIRYPKPDGVVSFDRLSSVFI
                     SNTNHAEDQPCHLTLRDASVPIKVNLELYDAPEQRYCPAGVYEIVRDADGKNPRLQIN
                     AQNCVHCKTCDIKDPTQNIVWVAPEGGGGPSYPNM"
     gene            5940..6812
                     /locus_tag="AC16CX_15550"
     CDS             5940..6812
                     /locus_tag="AC16CX_15550"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3013957.1"
                     /translation="MATSQPKNGNPPPGADTGQAQGEGGQAVYLRLMNEQLVGWDDPR
                     GTLQRALAENQFLLLAQKILPLRSGAPDPACYEVLLRLQKEEDNLLPPGGFFDIAEGL
                     GMMGDIDRWVVRNVIAWGAAAMKANPSSPLPMLCVNLSAAALVDRSFANFVRGQLNKS
                     GYPPRALCFEISERDVIDLHDDTQKFIAALKPGCRITLDAFGSVKVSFSHLSGLAIDF
                     IKIDGVITQNILRDPASLAKVRAIHAACRKIGVRAIAEFVETREMLDKMREIGVDYVQ
                     GFGIARPEPISKIQ"
     gene            complement(6955..7530)
                     /locus_tag="AC16CX_15555"
     CDS             complement(6955..7530)
                     /locus_tag="AC16CX_15555"
                     /inference="COORDINATES: protein motif:HMM:NF027363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HIR3013958.1"
                     /translation="MKSVLAGIITGAWLVLGAAFAAEVEGVKLADKIRVGDAGPELIL
                     NGAGVRTRVFFKVYVGALYLQRKMDSTQAVLADSGARRVAMHMLRDLTAEQLFSALDD
                     GLKKNHTPEQLAKLEPQVRQLEGIFNAVKAAKPGDVILLDYSPGAGTRVTVNGEAKGT
                     IPGEEFNRALLRVWLGDQPADAALKKAMLGG"
     gene            complement(7670..8869)
                     /locus_tag="AC16CX_15560"
     CDS             complement(7670..8869)
                     /locus_tag="AC16CX_15560"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3013959.1"
                     /translation="MAKQVQDAYIVAAVRTPVGKAPRGMFRNVRPDDLLAHALKSALA
                     KCPALDPAVIEDVIVGCAMPEAEQGMNVARIGLLLAGFPNTVSGMTINRFCSSGVQAV
                     VLAADRIRLGEAEVMVAAGTESMSMVPMGGNKPSFSPAIFETNENVAIGYGMGITAEK
                     VAAQWKVSREEQDLFAAESHRRAVRATDTGEFKDEITPYSIAERLPDLAAREVRVNAR
                     EAVHDEGPRRDTSPEALAKLRPAFAMKGSITAGNSSQMSDGAAAVVLMSEAALARYNL
                     RPLGRFMGYSVAGVPPEIMGIGPVKAIPKALKQVGLKQDDIDWIELNEAFAAQSLAVI
                     KDLGLDRTRVNPLGGAIALGHPLGATGAIRVATLMHGLRRHRKKYGMVTMCIGTGMGA
                     AGIFEAL"
     gene            complement(8961..>9278)
                     /locus_tag="AC16CX_15565"
     CDS             complement(8961..>9278)
                     /locus_tag="AC16CX_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3013960.1"
                     /translation="RALAAAGYRPPLRQVAVPAAGRNAIATIRAFMENLLAGGHISEH
                     DYLIGHKVATVMCGGEIEGGSLVDEQWFLDLERKHFMELIATEKTQARIEHTLKTGKP
                     LRN"
BASE COUNT         1621 a         3051 c         2983 g         1623 t
ORIGIN      
        1 cacaaacgct actccggctt cgacgcgctc aagctctatt gccaccgcgt ggcgggcgtg
       61 gtcgggctgc tttcagcgga aattttcggc taccgggacc ggcgcacgct ggattacgca
      121 gccgacctcg gcctggcgtt ccagctcacc aacatcatcc gcgacgtggg cgaggacgcg
      181 cgccgcaacc gtgtttacct gccgctcgac gagatcgggc gttacggggt aagcgagtcc
      241 gacatcgtgc aggcgcgcga aaccgacaat ttccggcgcc tgatggaatt ccagatcgag
      301 cgcgcgctcg gctattaccg tgaggccttt gccaagctgc cggcaatgga ccggcgcacg
      361 cagcgcgccg gcctcgtgat ggcggcgatc tatcaggcgg tgcttgacga gatccgccgc
      421 gacggctgcc gcgtgctgac ccggcgcgtg accctcacgc ccatccggaa gctttggata
      481 gcgtggaaaa catggataag gggataaggc gtgacgtgat gaatcgtgac gtggtgattc
      541 gtgacgtgat acggcgtgac gtgataaagc gtggttttgg tttcaatgac gcttttccat
      601 gtcacgctcc ctcaagtcac gcgtcaacat gttacctgta ctaggtcacg cttctcgata
      661 ggataatcga tggatctcca gttgaaaggg aaaaccgcgc tggtcaccgg agcgagcatg
      721 ggcatcgggc gcgccatcgc caagggactt gcagtggaag gagtcaaggc ctgcattgcc
      781 gcgcggcgca agccgctcct tgcgaaactg gcgaaggaga tcgcagcagc aggcgggccg
      841 gagcctttcg tagtcgaagt ggacatcctg cggcaaggcg cgccggaaaa actggcgcaa
      901 gctgcacagc gcgcgctggg aggcatagac atcctggtga actgtgcagg cggcagtaag
      961 ccgccggtcc cgatcgacgc gccggaagcg gaatgggacg aagcgatgac cctcaacttc
     1021 acgcgcctgc gccagctcac gcacgccgtt ctgcccgtga tgatcaggag aaagtggggc
     1081 cgcgtcatca acatctcggg caaatcggaa ccggagcggc tgattgccgc gcacgccgcg
     1141 aaagcggccg tacacgcctg gtccaagggc ctgtcgcggg aagtcggcag atacggcatc
     1201 acggtcaatt gcatccagcc cggccgcatc ctgagcgagc agatcctgcg caaatattcc
     1261 ccggagtacc gcaaggaaca gtccgaaaag gagataccgg tcggccgcta cggcgagccg
     1321 gaagagctcg cctgtctcgc ggtcttcctt gcctcgcccg tcgcctgcta catcaccggg
     1381 gcggtgatgc cggtcgatgg cggcctcagg cgctatgctt tctaaatgcg tgaatagtga
     1441 gtagtgaatg gtgaatggac tgaaccgttt gccgctggtt tttccactca ctactcacca
     1501 ctcaccactc acgcctttgg tcgtataatc agacgagttt tcccggagat ctcgcatgca
     1561 aacgaaaatc ccgtgtgtgg tgatgcgcgg cggaacgtcg cgcggcccgt tcttccacgc
     1621 gtccgacctg cccgccgacg cgcccacgcg cgacgcggtg ctgctcgcgg tgatgggctc
     1681 gccgcacgag taccaggtgg acggcatcgg cggcgcggtg tcggtgacga gcaaggtcgc
     1741 gatcatcagc aagtcgaagc atcccggggc cgacgtggat tacctgttcg cccaggtgct
     1801 gatcaacgag aagctggtgg acaccaagcc gaattgcggg aacatgctgg tcgcggtggc
     1861 gccgttcgcg atcgaggagg ggctggtgcc ggcgaagaac ggcgagacga cggtgcgcat
     1921 cttcaacgtc aacacccaat cgctggtcga gtccatcgtg cagacgcccg gcgggagcgt
     1981 cacctacgcc ggcgactctg cgatagacgg cgttcccggg accgcggcac ccgtcaagct
     2041 caatttcaag agcgcgattg gctcggttac cggcaagatg ctgccctccg ggaaaccgct
     2101 cgacgtgata gatggcgtgg aagcgagctg cgtggacgtc gcgatgcccg tggtcctgat
     2161 gcgggcggaa gcgttcggca agaccggcca cgaggccgcg gttaagctcg acgcggacct
     2221 ggaactctta agacgcatgg agacgatccg ccgcaaggcc ggcgcgctga tgggcatggg
     2281 cgacgtctcc aagctggtgg tgccgaagat ggccctgctg tcgaagccgc gcaggggcgg
     2341 cgccatcgcc tcgcgcttct tcgtgcccga tgcctgccac aaggcacacc cggtaaccgg
     2401 gaccgtgtgt atcgcctcgg cctgcgccat atccggaacg gtggcatcgc agatctcccc
     2461 tctgccgccg gcgcctcagg gcatgatcag gatcgagcat ccctcgggca tgatcatcat
     2521 cgatctcgac gtcgatttca ccggcggcaa acaggagttg cgccgcgcag ccctggtgcg
     2581 caccgcgcgc cggattttcg aaggctacgt gaacgtgccc gagcaggtct ggtcgggcga
     2641 cacgcgcgcc gcgaagccgg ccaccgcagc agtctagccg ccggcagcgc acttctctca
     2701 tgaatgtggc cgtgatcggc ggtggttacg ccggcatggc ggcggccgtc acgcttgccg
     2761 agcgccgggt tcccgtgacg gttttcgaag ctgcgaagac cctgggcggg agggcgcggc
     2821 ggattgatca ccgtgatgtc gcgctcgaca acggcctgca catcctgatc ggcgcctaca
     2881 gcgagacgct ccgcctgatg cgccaggtgg gtgccgaccc ggagcggctg ctgctgcgcc
     2941 tgccgctcac ctggcggata cacgagcgtt tctcgctgaa ggccgtttcc ctccccgccc
     3001 ccctgcacct gctgggcggg ctcgccaccg cacgcggcgt tccgcttgca gaacgcctcg
     3061 ccgccgcgcg cttcctgcgg cgcatgcggc ggcagggcta tgccctgccg caggacacca
     3121 ccgtcgcgtc gctgctcgag agcgaagcac agggcgggcg catgacacgc ctgttgtggc
     3181 ggccgttgtg cgtctcagcg ctcaacacgc cgtccgaggc ggcctctgcg caggtgtttc
     3241 tcaacgtgct tcgcgacagt ctcaatgccg atcgtgcggc gagcgacctg ctgttgccgc
     3301 gcgtcgatct ctcgcggctc tttcccgagc ctgcggccga ttacgtcaag gcgaacggcg
     3361 gcaccacgct gatcgggcaa cgcgtgaccg agatagaaga gagcagcatc ggcttcacgg
     3421 tgcgttgcgc cggggtcgag cgcagcttca cccacgtcgt gtgcgccctt ccgccttatc
     3481 acgcgggggc gttcctgatc ggcatctcgg cgctggccga ggcggctgaa atcatcgaag
     3541 ggctgaccta tcagccgatc tactcggtct atctcggtta tccacgggaa gtgcggctgc
     3601 cggccccgat gctggggttc gagagcgcac tgctgcaatg ggcgtttgac cgcggcgcgc
     3661 ttggcggaca gcacggcgtg atcggcgcgg taatcagcgc ggagggcccg caccaggacc
     3721 tggcgcagga cgagctgggg cgcctcgtcc atcaggagct tcaacagcag ctcgggattc
     3781 tgagcgagcc cctgtggtgc cgagtgatcg ccgaaaagcg cgccactatc gcctgcacgc
     3841 cgggactcaa gcggccggag tcgcgcacgc cgctccggga tttcctgctg gcgggcgact
     3901 acgtggcgag cgaccatccc gctacgattg agtctgccgt gcgcagcggt gtaaacgcgg
     3961 cccgcatgat actcggctca aaggcgtgat tcgtgaatag tgagtggtga ctggactgaa
     4021 gcccgaaagc gtcgtctgtc cgaatgactt gagtccgctc actactcacc actcactact
     4081 cacgctcttg cggcgcatga ggccatgacg cgccttataa tggcgccagg ttttcattgg
     4141 gggaaagagt ggccgaacgc gaatccatgc agtatgacgt cgtggtggtg ggcgccggac
     4201 ccgccggcct cgccgcggcg atccgcttga agcagctcgc tgcacagcac cagcacgaga
     4261 tcagcgtttg cgtgctggaa aaaggctcgg aggtcggcgc gcacatcctg tccggcgcgg
     4321 tgatcgaccc gcgcgcgctc aacgagctgc tcccggactg gcagaggctc aacgcccccc
     4381 tcaacgcgcc ggtcaccgaa gaccgcttcc tgttcctcac cgaatccgct tcgttcaagg
     4441 tgccggatat catgctgcca gcgtgcttca ggaaccacgg catgtatgtc gtcagcctgg
     4501 gcaacgtgtg ccgctggctc gcgcagcagg cagaggggct gggcgtcgag atcttcgccg
     4561 gcttcgccgc cgcggaagtg ttgcacgaca ggaacggcgc ggtgctgggc gtggccaccg
     4621 gcgacatggg catcggccgg gacggaaaac cgaccgcgaa ccaccagccc ggcatggagc
     4681 tgcgcgcccg ctacaccctc ttcgccgagg gctgccgcgg ccacctcggc aagcggctgc
     4741 aggaccgctt caacctgcgc gcaggcgccg atccgcaggt ctacggcatc gggctcaagg
     4801 aactctggga agtgaggccc gagcggcacc ggcaagggct ggtcatccac accgcgggct
     4861 ggccgctcgc aacggacacc tatggcggct cgttcctcta ccacctcgaa aacaaccagg
     4921 tgtcgatcgg cttcgtggtg ggactggcct acagcaaccc gtatctgagt ccctacgagg
     4981 aattccagcg cttcaagacg caccccgcca tccggccgtt tctcgaggaa ggcaagcgca
     5041 tcgcctatgg cgcgcgggca atctccgccg gcgggctgca atcgctgccc aggctcgtct
     5101 ttcccggcgg ctgcctgatc ggcgacgatg cgggcttcct caacgcctcg cgcatcaagg
     5161 gcagccacgc ggcgatgaaa tcgggaatgc tggcggcgga ggcggcgttc accgcgctgc
     5221 gcgcgggacg ctcgcatgac gagctcggcg cctatcccga ggcgttccgc gcgagctggc
     5281 tgcacgacga gctccatcgc gcgcgcaact tcaagccgtg gatgagcaag gggctcgtgc
     5341 tcggctcgct gatggtgggc gtggaccagg tcgtgttcca gggacgggcg ccatggacgc
     5401 tccatcacgc ccacgccgac cacgaaacgc tcaggacgaa gtcggaggcg gagccgatac
     5461 ggtatcccaa gcccgacggc gtggtgagct tcgaccgcct gtcctcggtc ttcatctcga
     5521 acaccaatca cgccgaggac cagccctgcc atctcacgct cagggacgcg tcggtcccga
     5581 tcaaggtcaa tctcgagctc tacgacgcgc cggagcagcg ctattgcccg gctggcgtct
     5641 acgaaatcgt ccgcgatgcc gatgggaaaa atccgcggtt gcagatcaac gcccagaatt
     5701 gcgtgcactg caagacctgc gacatcaagg acccgacgca gaacatcgtc tgggtcgcgc
     5761 ccgagggcgg tggcggaccg agttacccga acatgtaacc cggttgtccg catgcgcgcg
     5821 catggacgcg actcgccgaa ttgcgcgcag gttttccggg cttcgctgca agtgcatggg
     5881 ttagaatagc ccgcccggac tcaacccggg aaacacccca aataccgata gcccccggca
     5941 tggcaacgtc tcaaccaaag aacgggaatc cgccgccagg cgccgatacc ggccaggcgc
     6001 aaggagaagg cggccaggcg gtttacctgc gcctgatgaa tgagcagctg gtgggctggg
     6061 acgacccgcg cggcacgctc cagcgcgccc tcgcggaaaa ccagtttctg ctgctcgccc
     6121 agaaaatcct gccgcttaga tcgggggcgc ccgacccggc ctgctacgag gtgctgcttc
     6181 gattgcaaaa ggaggaggac aacctgctgc cgccgggcgg gttcttcgac atcgcggaag
     6241 gcctcggcat gatgggcgac atcgatcgct gggtcgtccg caacgtcatc gcgtggggcg
     6301 ccgccgcgat gaaggccaat ccgtcatccc cgctgcccat gctgtgcgtc aatctgtcgg
     6361 ccgcggctct ggtggaccgc tcgttcgcga atttcgtccg cggccaactg aacaagtccg
     6421 gctatccgcc gcgcgccctg tgcttcgaga tcagcgagcg cgacgtcatc gaccttcacg
     6481 acgacaccca gaagttcatc gccgcgctca agcccgggtg ccggatcacg ctcgacgcct
     6541 tcggcagcgt caaggtgtcg ttctcccatc tctccgggct ggcgatcgat ttcatcaaga
     6601 tcgacggcgt gatcacgcag aacatcctgc gcgatccggc cagcctggcc aaggtcaggg
     6661 cgatccacgc cgcctgccgg aagatcgggg tgcgcgccat cgccgagttc gtggaaacca
     6721 gggagatgct cgacaagatg cgcgagatcg gcgtggacta cgtgcagggc ttcggcatcg
     6781 cccgcccgga gccgatcagc aagattcaat gaatcgtgaa tggcaattgg caggcagcgc
     6841 ataggggttc gggcgatcct ctagcgcgcc attccccagg gcctcgaatc attcacgatt
     6901 cattattcaa gacttgttat ttggcttgtt aaggctgacg tggcactcgc cgcgtcagcc
     6961 tccgagcatc gccttcttga gcgcggcgtc ggcgggctga tcccccagcc agaccctgag
     7021 cagcgcgcgg ttgaattcct cgccggggat cgtgcctttg gcttccccgt tcaccgtgac
     7081 gcgggtgccg gcccccggag aatagtcgag cagtatcacg tcgccgggct tcgcggcctt
     7141 caccgcattg aagatcccct cgagctgcct tacttgcggc tcgagcttcg ccagctgctc
     7201 gggcgtgtga tttttcttca gcccgtcgtc aagcgccgaa aacagctgct cagcggtgag
     7261 gtcgcgcagc atgtgcatgg cgacgcgcct ggcgccggaa tcggcgagca cggcctgcgt
     7321 gctgtccatc ttccgctgca ggtaaagcgc gccgacgtag accttgaaga atacgcgcgt
     7381 gcgcaccccg gcgccgttca ggatgagctc gggcccggcg tcgccgaccc ggatcttgtc
     7441 ggccagcttg acgccctcga cttcagccgc gaacgctgcg cccaatacca gccaagcgcc
     7501 tgtgatgata ccggccagga cggatttcat atgttcctcc gcgttctcgg ctggaaatgc
     7561 gtgaatagtg agtagtgaat ggtgattgga gcaggctgta aagcgagtat caacggtggt
     7621 tcgctctact cactactcac caatcaccac tcacgcatct tcgaggccat caaagcgcct
     7681 cgaagatgcc ggccgcgccc atgccggtgc cgatgcacat ggtgaccatg ccgtactttt
     7741 tccggtggcg gcgcaggccg tgcatcaagg tcgccacccg gatcgcgccg gtcgcgccga
     7801 gcgggtgccc gagcgcaatc gcgccgccga gcggattgac gcgggtgcgg tcgaggccga
     7861 ggtccttgat caccgcaagt gattgcgcgg cgaacgcctc gttcagctcg atccagtcga
     7921 tatcgtcctg cttgagcccc acctgcttca gcgccttcgg gatcgccttg accggcccga
     7981 tgcccatgat ttccggcgga acacccgcca ccgaataacc catgaaacgg cccagcgggc
     8041 gcaggttgta gcgcgcgagc gcggcctcgc tcatcaacac gaccgccgca gccccgtccg
     8101 acatctgcga gctgttgccc gcggtaatgc tgcccttcat ggcgaatgcc ggcctgagtt
     8161 tcgcgagtgc ctccggagaa gtgtcccggc gcggcccttc gtcgtgcacc gcctcgcggg
     8221 cattcaccct cacttcccgc gccgcaaggt cgggcaggcg ctcggcgatg gagtacggcg
     8281 taatttcatc cttgaactcg ccggtgtcgg tcgcgcggac ggcccggcga tggctctccg
     8341 ccgcgaacag gtcctgctcc tcgcggctga ccttccattg ggccgccact ttctcggcgg
     8401 tgatgcccat gccgtagccg atcgcgacat tctcgttcgt ctcgaaaatc gctggactga
     8461 aggacggctt gttgccgccc atcggcacca tgctcatgct ctcggtgccc gcggcgacca
     8521 tcacctccgc ctctcccagc cggatgcggt ccgcagcgag caccaccgcc tgcacccccg
     8581 aggagcaaaa gcggttgatc gtcatgcccg agacggtgtt gggaaaaccc gccagcagca
     8641 gcccgatgcg ggcgacgttc atgccctgct ccgcttccgg catggcacag ccgacgatca
     8701 cgtcctcgat gaccgcgggg tcgagagccg ggcactttgc cagcgcgctc ttcagcgcgt
     8761 gcgccagcag gtcgtcgggc cgcacgttcc tgaacatgcc gcgcggggct tttccgacag
     8821 gcgtgcgaac ggctgcgact atgtaagcgt cctggacttg tttggccatg acggttctcc
     8881 tcatcaaggc gaaagtcgaa agtcggaagt cgaaagtaag cgcacgatct cggctttcct
     8941 ttcgcctttc gcctttcgcc ttagttccta agtggtttcc cggtcttcaa tgtgtgctca
     9001 atcctggcct gggtcttctc ggtggcgatg agctccatga aatgcttgcg ctcgaggtcg
     9061 aggaaccact gctcgtccac gaggctgccg ccctcgatct cgccgccgca catcacggtc
     9121 gcgaccttgt ggccgatcag gtagtcgtgc tccgagatgt gcccgccggc cagcaggttc
     9181 tccatgaacg cgcggatcgt cgcgatcgcg ttgcggcccg ccgccggtac cgcgacctgc
     9241 cggagcggcg gccggtaacc ggccgcggca agcgcccg
//
LOCUS       DBNGWZ010000510         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1075888, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000510 DBNGWZ010000000
VERSION     DBNGWZ010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1075888"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC16CX_15570"
     CDS             complement(<1..668)
                     /locus_tag="AC16CX_15570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3013961.1"
                     /translation="MFWNILISVAIAYAAVLALVFLFQSKLVYYPEIGREVTLSPQSY
                     GLKFESVKIRTEDGETLDAWWVPAEDARGTILFFHGNAGNISHRLDYLLMFNRLRYST
                     LIVDYRGYGRSTGTPSEQGTYRDAEAAWDYARHARLAREQDIVIAGESLGGAVATWLA
                     AKVQPRAVLLFSSFTSATDLGAQVYWFLPVRLISRIGYDNLENLKRTRAPVLVAHSRD
                     DDIVP"
     gene            complement(694..1725)
                     /locus_tag="AC16CX_15575"
     CDS             complement(694..1725)
                     /locus_tag="AC16CX_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3013962.1"
                     /translation="MEAKSKVESRKSKVQGQGRRAGRFFFRLSPFAFLLGCALATAAS
                     AQQYPDRPIRLIVGFPPGGAADILGRHAAQQLTEALCQQVVVDNRGGAGGLIATETAA
                     RANPDGYTLLFTSIPHVINPHLYRKVTYDAVKDFVPVVQFVAVPLMMASHPALPVKSV
                     KELIAYAKASPGKINYGSGGSGSSSHLAMELFKSMAGVDLVHIPYKGVGPMITDMLGG
                     QVRLTISSAVPLSPQVKTGKLRGLAVTSPKRSPSFPDLPAIAETVRGYEVVNWFGILA
                     PAGTSKAIVARVNKDLSAALASPALKERLAAQGADAVGGTPEDFGKVIRADFAKWAKV
                     VKESGARVD"
     gene            complement(1728..>2241)
                     /locus_tag="AC16CX_15580"
     CDS             complement(1728..>2241)
                     /locus_tag="AC16CX_15580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3013963.1"
                     /translation="RHGSTGVGSPHHLAAEIFKSMTGVKMIHVPYRGASPALIDVVAG
                     HIEIYFGAMVSTIPHVKSGRLRALGVTSLKRVAAAGGTPTLDEQGLKGFETGSWFGMA
                     VPAGTSRDIIMKLHAESVRAIAAPDIRDRMSAEGAEFVGDTPEQYAAFIKSEIAKWAK
                     AVKASGAKPE"
BASE COUNT          385 a          754 c          710 g          392 t
ORIGIN      
        1 ggcacgatat cgtcgtcccg gctgtgcgcg acgagcaccg gcgcgcgggt tcgtttcagg
       61 ttctccagat tgtcgtaccc gatgcggctg atcaggcgca ccggcaggaa ccagtacact
      121 tgcgcgccga gatcggtcgc cgaagtaaag gacgagaaca acagcacggc gcggggttgc
      181 accttcgcag cgagccaggt cgcgaccgcc cctcccagcg attctcccgc gatcacgatg
      241 tcctgctccc gtgcgagccg cgcgtgacgc gcgtaatccc acgccgcctc cgcgtcgcgg
      301 taggtgccct gctccgaggg cgtgcccgta cttctgccat agccgcggta atccacgatc
      361 agtgtggaat agcgcaagcg gttgaacatc agcaggtaat cgaggcggtg cgagatgttg
      421 ccggcgttgc cgtggaagaa caggatggtg ccgcgcgcat cctcggcggg cacccaccac
      481 gcgtccagcg tctcgccgtc ttcagtgcga atcttgaccg actcgaactt gagcccgtag
      541 gactgcggcg agagcgtgac ctcgcgtccg atttcgggat agtagacgag cttggactgg
      601 aacaggaaca cgagcgccag caccgccgcg taggcgatcg cgactgaaat caaaatattc
      661 cagaacatgc gcagcatcga agctggaacg gcactagtcg acgcgcgcgc cggattcctt
      721 gaccaccttg gcccatttgg cgaaatccgc gcggatcact ttgccaaagt cctccggcgt
      781 cccgccgacg gcgtccgcgc cctgcgcggc aagccgctcc ttgagcgccg gggatgcgag
      841 agccgcactc agatccttgt tgacgcgcgc gacgatcgcc ttcgaggtcc ccgccggcgc
      901 caggatgccg aaccagttga cgacctcgta cccgcgcacg gtctcggcga tcgcaggcaa
      961 gtcgggaaac gacggcgagc gcttcgggct cgtgaccgcg agaccgcgca gcttcccggt
     1021 tttcacctgc ggtgagagcg gcaccgcgct ggaaatagtg agtctcacct gtccccccag
     1081 catgtccgtg atcatcgggc cgacgccctt gtacgggatg tgcacgaggt cgacgccggc
     1141 catgctcttg aacagctcca tcgcgaggtg gctggaggaa ccgctgccgc ccgagccgta
     1201 attgattttt ccgggggagg ctttcgcgta cgcgatcagc tccttcaccg acttcaccgg
     1261 cagagcggga tgggacgcca tcatcagcgg taccgccacg aactggacca ccggcacgaa
     1321 gtccttgacc gcgtcgtacg tgaccttgcg gtaaaggtgc gggttgatca catgcgggat
     1381 cgaggtgaac agcaacgtgt agccgtcggg gttggccctc gctgcagttt cggtggcgat
     1441 cagcccgccc gcgccgccac ggttgtccac caccacctgc tggcagagtg cctcggtcag
     1501 ctgctgggcg gcgtgacgtc ccaggatgtc cgcggcgccg cccggcggga agccgacgat
     1561 cagccggatc ggtcggtcgg gatactgctg cgcgctcgcg gcagtagcga gcgcgcagcc
     1621 aaggaggaag gcgaaaggcg aaaggcgaaa gaaaaaccga cccgctcgtc gcccttgccc
     1681 ctgcactttc gactttcgac tttcgacttt cgacttggct tccattccta ttccggcttc
     1741 gctcctgacg ctttcaccgc cttggcccat ttcgcgattt ccgacttgat gaacgccgcg
     1801 tactgctcgg gcgtgtcgcc gacgaactcg gcgccttcgg cgctcatgcg gtcgcggatg
     1861 tccggcgccg caatcgccct caccgactcc gcatgcagct tcatgatgat gtcgcgtgag
     1921 gtcccagcgg gcaccgccat cccgaaccag gagccggttt cgaatccctt cagcccctgt
     1981 tcgtcgagcg tgggtgtgcc gccagccgcg gcgacgcgct tcaggctggt gacgcccagc
     2041 gcgcgcaacc gcccgctctt cacatgtggg atcgtggaca ccatcgcgcc aaaatagatc
     2101 tcgatgtggc ccgccaccac gtcgatcagc gccggcgagg cgccccggta cggcacgtgg
     2161 atcatcttca ccccggtcat gctcttgaag atctcggcgg cgagatggtg cgggctgccg
     2221 accccggtcg agccgtgccg c
//
LOCUS       DBNGWZ010000511        18421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1955983, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000511 DBNGWZ010000000
VERSION     DBNGWZ010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 18421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18421
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1955983"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC16CX_15585"
     CDS             complement(<1..192)
                     /locus_tag="AC16CX_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013964.1"
                     /translation="METSVARQSEISRDAISADALIETIRQLAAELHPSRAVIPSSLD
                     GRLEQDYGFDSLGRVELFLR"
     gene            complement(321..1763)
                     /locus_tag="AC16CX_15590"
     CDS             complement(321..1763)
                     /locus_tag="AC16CX_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013965.1"
                     /translation="MIAVASCAVLIFAQPAIATAQDKIPAEQYDRAPLGTIGLAVGQF
                     QPTIDFDALIRGRGDAAGKGALSGAGTCFELLRGSASGAAAGAAALVFLACLPIGATI
                     GAISADRRAAPSETIDSAESSLKARLADVNIQGNMRKALMEYAQKNGLRIGALAPELG
                     PVSADAAATYSTGTSGMDTVLEVSLTKITSRTTGTKELLIGFEISVNVRLVQLSVNKL
                     LDNFTHIYRGPHRTWEEWSAHGGKLIDEDFEQGYRDIAEQVLDEVFLIYRPTAPVATG
                     KESALTFPGEENFAKENTPIRGKVPLYTLRPVYPEVRGKVYFSGPSIYGLLERVKVDS
                     IRPALRWEAFPRAHDVPLSGESISRISEVTYELKIYESVLGRLGGPVILAGQEIYSRP
                     GLTAPAHQVEVDLRNCNVYFWTVRARFKLDGIYRVTEWMGAYNTMGGEADPRWWRRSE
                     FPPLVFRFANKMYYLPFQTPAKEGVDCPLN"
     gene            complement(1960..2481)
                     /locus_tag="AC16CX_15595"
     CDS             complement(1960..2481)
                     /locus_tag="AC16CX_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013966.1"
                     /translation="MELRNFSRYSLLRKALVLSLPVLALSGCYVMPVGQDSSGNPYFV
                     YSPLPIPPVVPAPGHTQGPQPVAVPGGPMPATLPVKLYPINDLANQTGVLTGQVTNMM
                     TGKGRFTFSYQGETLVGEATRIANDERRGVASAYGPRGTFARCEYQMSSAFLGAGTCT
                     FSNGAQYQVHIGS"
     gene            complement(2577..2795)
                     /locus_tag="AC16CX_15600"
     CDS             complement(2577..2795)
                     /locus_tag="AC16CX_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013967.1"
                     /translation="MTTKTESRRSQARELPHDRRADEILEFVFNDEEDALCNERFALT
                     HQRDMPALQEYLREMFELDSRSGDVRWH"
     gene            complement(2964..4013)
                     /gene="lgt"
                     /locus_tag="AC16CX_15605"
     CDS             complement(2964..4013)
                     /gene="lgt"
                     /locus_tag="AC16CX_15605"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3013968.1"
                     /translation="MIGPYVHDIDPVIGSLFGLHLWWYGLAYALGFLAIYIFLWSRRR
                     ELGLTAGRVYDLSILVAAGVLIGGRLVEVVFYEWPFYRGHPHLIPAYWLGGMATHGLL
                     LGALAGILLFCRIHRQSFLVTADALAVPAAFILGFGRIGNFIDGLIVGSPTDAWWAVK
                     FPDAEGFRHPVVLYDAIKNLLLVPVLLLVYRQRPAPGVVTGLFLFGYAGLRIVVDLFR
                     EYPTTLLGLATGQILNIAMSLAGLALLAWCITRQSSVSRGIRGEENTPSPKRGTNRTG
                     WRLAVLATLVAFSLVIPSDWTQDVPDRYGKRHPGLQHSALYPAISGLSAAPDVATSLA
                     ETAGRPPNEPSAGSP"
     gene            complement(4045..4635)
                     /locus_tag="AC16CX_15610"
     CDS             complement(4045..4635)
                     /locus_tag="AC16CX_15610"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3013969.1"
                     /translation="MKLIDYGINLVLSVILIVGVYQFYFWCQRNYLATPRELKLRLDE
                     WIPYWPSWVWIYSCIYYPLILYLNFVLESPRQFTYVAMSYVLLLGLQMSFFVLFPVTT
                     PEQWRSYNQQRTLSERFLAFVQRFDARSNSFPSMHTSVAMLTALHLLPHLGALAFAFP
                     VLIALSCLFTKQHYVLDLPAGALLGWLTFHAYQNLS"
     gene            complement(4655..6508)
                     /locus_tag="AC16CX_15615"
     CDS             complement(4655..6508)
                     /locus_tag="AC16CX_15615"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR3013970.1"
                     /translation="MSTAMAPVATGTNSSAPVRLRAWLGIGENSLLAGALAAIVLLPL
                     AEAVLRKTLNAGITSSASLVQHLTLVASMIGAAVAARDGRLLTLFDVSALFGAHAQRI
                     GHFASRTLAAAVCGLLCIASLQLVAAERAAGNVLAYQIPVWAAQSVLPAGFALIALHI
                     AWRASEGIYARVLSVCLTVALLACAPLWAAHPALWFYPALLLLVASTLTGTPIFAVLA
                     GASLFLFSQQGAPIASVSLDHYRLVVNPSLPAVPLFTLAGFILAAGGAPARLTRVFEA
                     LFGHFRGGVAIAAVTVGAFFTAFTGASGVTILALGGLLLPLLVSARYRERDALGLVTG
                     SGSLGALLPPCLPLVLYAIVAKVSIERMFLGALLPGLLMIVLAAWWGARRDPRSRQDI
                     APFDWDEAKRALYAAKWELALPVVALAGLLGGFATPVEAAALTALYAFIVETLVHGDL
                     RESRRLLAVTTECGALVGGVLLILGVAMGLTNFLVDIQLPERAVAWATQTIQSKWLFL
                     LALNVLLLAVGCLMDIFSAIVVVAPLVVPIGLAFGVDPVHLGVIILVNLELGYLTPPV
                     GVNLFYASSRFNKSIAEVCRSVAPLLPVLAFGVLTVTYLPWLSTALPAAFR"
     gene            complement(6505..7548)
                     /gene="dctP"
                     /locus_tag="AC16CX_15620"
     CDS             complement(6505..7548)
                     /gene="dctP"
                     /locus_tag="AC16CX_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR3013971.1"
                     /translation="MAISKFMRHFGLITALCLLPGVTAAAAAQDAARIKLGTIAPRGS
                     IYHKSLMEMGETWRRAEAPGASFTVFPDGSQGGEADIVRRMRIGQLNAALISVIGLTE
                     IDPGAGALQKMPLMFRSWEEVDAVGQRVRPLLEKRFLDKGFVVLFWAEGGWVRFFSKE
                     RALRPADFRRLKMFAWTGDAEQIAQMKALGYQPVVLETTDILPGLQTGLINAVPVTAA
                     WALAGQIDVTAPHMLDIRWVPVVGAAVITRSAWDALSPSGKQALRRGAEKAAEQLRAQ
                     RATADAEAVEAMKKRGLQVHPLTPELETEWRQLAESVYPKVRGAMVPADMFDAVHEAL
                     AEHRGTTSNGAAR"
     gene            complement(7569..8528)
                     /locus_tag="AC16CX_15625"
     CDS             complement(7569..8528)
                     /locus_tag="AC16CX_15625"
                     /inference="COORDINATES: protein motif:HMM:NF028121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HIR3013972.1"
                     /translation="MTRNGTLSSGIACGAPAWKGWALSVALALCLGASGCSIRNYAMS
                     RVGDALAASGSGFGSDDDVELIGAAAPFSLKLMESMLAENPRHRALLLATAQGFVQYA
                     YAYVELKADEIEDTNLQAAHFQRERARRMYLRARDYGLRGLEAAHPGFTTTLKTRPLD
                     AVSKTSEADVGLLYWTGVAWAAAISLSKDDPFMIADLPAVEALVQHALALDDTHDHGA
                     IHVFMINYEMSRAGLRAGASARARAHFARAVELTGGRHAAPYVTLAEAVSIPEQNRGE
                     FESLLQHALELDASGNKEWRLVNLVMQRRARWLLARADRFFSE"
     gene            complement(8602..9966)
                     /locus_tag="AC16CX_15630"
     CDS             complement(8602..9966)
                     /locus_tag="AC16CX_15630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR3013973.1"
                     /translation="MRIGLIAMSGIRACDEELLALGLTLPGFVERSKAIASLPSLGLL
                     TLAGMTPGRHELRYIEVADLKRMESLPADFDLVAISSFSAQINEGYQLARRYQALGVP
                     VVMGGLHVTMNPQEPQRSGASAVLGEGEIVWPEILEDAERGQLKPVYDAHGREFDLAN
                     APMPAFGLLDLQRYNRITVQTSRGCPWRCSFCASSILLTRRYKQKPVGKVLAEIDRIR
                     ALWPRPFIEFADDNSFVNRRYWRELLPELEKRRIRWFTESDLAVHEDEELLHMMRRAG
                     CAEVLIGFESPMVQGLDGLELRRNWKHERYGQYLRAIERIQAAGIRVNACFVVGLDGH
                     DPSVFDALLAFVRDACPFDVQVTVPTPFPGTPFYAQLKAEGRLFGDEAWERCTLFDVN
                     FRPRGMTAEDLRAGLKRLVVALYSEEATRKRREHFRKRCLRNGRRHRATGESGLTRHS
                     AIFK"
     gene            complement(9963..10340)
                     /locus_tag="AC16CX_15635"
     CDS             complement(9963..10340)
                     /locus_tag="AC16CX_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013974.1"
                     /translation="MSGLNLWAAWIGILLGMASGMFQGLFFDRDDWLGGYSSWPRRLL
                     RLGHISFFGVAFLNLAYANTVWLLGPASHVPLASMLLVAGAMLMPLVCYLAAWKKPLR
                     HLFPLPVLALLGAALTFITGGLS"
     gene            complement(10704..16319)
                     /locus_tag="AC16CX_15640"
     CDS             complement(10704..16319)
                     /locus_tag="AC16CX_15640"
                     /inference="COORDINATES: protein motif:HMM:NF017658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion domain-containing protein"
                     /protein_id="HIR3013975.1"
                     /translation="MKIRHNRAAKTRQMKLISVAVTSCFASSLAFANPTGPTVVNGTV
                     SVVPMGNVLQITNSPNSIINWQSFSIGAGELTRFIQQSSASAVLNRVITQNPSTILGA
                     LQSNGRVFLINPNGIVFGAGSQIDVAGLVASTLSLSDADFLAGRMRFTEVPGAGSVVN
                     QGNITTPAGGQVYLVGPAVTNSGIITSPQGEVILAAGNSVELVDAGTPNLRVEIVAPD
                     NQALNLGQIIADSGRVGIYAGLINQSGRIQANTAVATEDGRIVLKATNSATFAPGSVT
                     TATGPLELEAGDITVASNITSGPQTITAGSGLIVQSSAAGPAQLSATGGQTINARFIE
                     VTAQDNNAAGIFNSTSGDQVITVAGGGLSPGIDVRSLASGGSASIGNNAPGAAQVINV
                     TDADHINVNGFGPNISNVSAIIHAIPGTQTISITGSGANAINVGSVGALGGSNINARS
                     QSITAGTSGQSGSINIVGPAVNSRLAAISTNVGFPGTQTVSTSGALTVTGGNAPAQTF
                     TSGINHNSNGPQTVTAARITLQGGLTGSGNSAQFFSASGAQTVSASEITLTGGASGSS
                     NVARIQNNSTGPQTITGSPIITITGGTGGIVDQGNSASIFSTGPQDITAASIDITAGG
                     AGFNNFAGINAPVQVITVRGDLTLTGGASASGPLRDGAARIGGRPNPATPTNLTLTVG
                     GDVTMNGGSMAFAGPVIGSHFLGGQPTNITMNVGGNLTLGTGTSANSSARIGSPSASS
                     AGGSISITAGGDITLENATNQSGTRSVIRTLDSLTLNANNITVGGSVISGPQTINAAD
                     DITVAAPNATNAIVTATGPQTITAGGTLTVQGGAGTGRSAQILGSAGQTISASSINVT
                     GGSVGNSSFAEIRQLGAAAGQSINASDTITITGGASGTDNFARIRNDGIGAQTVSVTG
                     PLGLTGGTGGAPDLGNFAQIRAIGSQNITAGSAMLAAGGAGFSNFAGIHSPDQDITIH
                     GDLTLTGGNSVSGPTSAGAARIGGIGGAIPGPTNLVLNVDGNFTTSGGTEASAGAVIG
                     SAFQGGQPTDIAMIVGGNVTLNPGTVANAGSRIGSPSGAPAGGNISVTAGGDLFLNSA
                     PGLVGSVIRTTDSIALQADSIVQGPNARIQAGSLATNTANGASLTGDNQVASFNATNT
                     TSGDVALNNATPLLTVTGVNQTSSGALTINQAGDLTLTGTISSGPQTFNATGSISVLP
                     GSPAFVNAFGPQSITAGEGLVVQSSAAGAAQLLASGGQTINARYIEVTAQDNPAFVSN
                     NLSGDQLITTTGMNASGEGLALRNLGGPGAFATIAQNVTGGRQQISVNNAERMTVDGI
                     GGNALIFDNSGIQAISITGAASANALNLGSPGSLGASQIGGGGTQTVTAGLAGEQGSI
                     TIIGKAAGSGGNSIIVSNAVPGGIQTVSTPGTLTVIGGTSPAGTSAAGIFANGIGGQQ
                     TINAGAIALQGGPGGSGNNAQIGALNGAQQITVGADGISLLGGAGGTNNAAVIFGATQ
                     DISTMGNVTLGGGFGSGNSARIGGRGGANPGPTDLTLSAGGNVALTGNAASNTVAVIG
                     SAGAGPLELTQIAINAGGNVVLSGGTGAGARIGSPAPATGGDISITAGNAIQVNGNTR
                     AATIRTLGNVQLHADSPGGTISEDPNGAVQANALITTSNGNTSLTGANLVSAFNASSV
                     SGNVALNNTSPLLTVTGISQAPVGALTLNQTGDLAITGNVVSGPQSISATGDITVVAG
                     SSTTLSAAGPQMLNAGGTLLVQGGATSGGSASITGNGPVSVSTGGDLSLMGGSGQDAY
                     ALLFSNGDINLTVGGALRMNQGTGERSWARVQTATRDSVISIFFPNLSSGGFFVNDFE
                     GALRRGQTGLLSGEGVAVPDHTLIIEYAQ"
     gene            16585..17289
                     /locus_tag="AC16CX_15645"
     CDS             16585..17289
                     /locus_tag="AC16CX_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3013976.1"
                     /translation="MSKRTGALVRVMIVEDDPATLERFAGAISRDPRTRVVEKARTGS
                     EAIARLVSVRPDVLLVDLGLPDMHGTRVISHAVRVHPACDVMVITVFGDERNVLASIE
                     AGATGYVLKDCSDDELIAKVLELRTGGAPMSPGIARLVLSRMHRQDRSRSKQTAAGGE
                     HAILTEREVEVLSLIARGYLYAEIAKRLGISLNTVTSHIKNSYRKLAVHSGAAAVTRA
                     AELGLLRSDDDKGTAR"
     gene            complement(17208..18257)
                     /locus_tag="AC16CX_15650"
     CDS             complement(17208..18257)
                     /locus_tag="AC16CX_15650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3013977.1"
                     /translation="MPVPMMRVAITQLVGREPGSFLYGWGVTVTIFYSTVLLVLVPAG
                     WWLLSEEAPSSRPTLWYREWFPWIWIAILVGGQALSVLPLGIATIERHRLTMRELKRR
                     VTEKAREIEADHACVEEARREQAIARERQRILADMHDGLGASLVGLLRHVQSADADRA
                     SIERRVQEALQEMRIAIDALQPWEGDLAAVLGSLRYRLDGMIRLSGVSFAWDVEELPD
                     LGALKPADVFALQRTLLEAITNALKHSGARHLRVTARGRDADVEIRIEDDGCGFNPSQ
                     FSAGLGLANMRARAQRIGARLTIQSQPGMGTNVRLLIPRVMAGCAGAQRAVPLSSSER
                     SRPNSAARVTAAAPE"
BASE COUNT         3359 a         5763 c         5947 g         3352 t
ORIGIN      
        1 gcgcaggaag agctctaccc ggcccaagct gtcgaagccg taatcctgtt cgagccggcc
       61 gtcgagcgag gaggggatga cggcgcgcga ggggtgcagt tcggcggcga gctgccggat
      121 cgtctcgatc agcgcgtcgg cgctgattgc gtcgcggctg atttcgcttt gcctcgcgac
      181 ggaggtttcc acggcaggct ccacgccgga agggtggggc gatgttagca cacgctcaaa
      241 ctggcgcgtg tcacgcgcct acgatttgca tttatgacga attactgccg ccgatctctg
      301 gcttagtccc catttagggg tcagtttaat gggcaatcga caccttcctt agccggagtc
      361 tgaaacggca ggtagtacat cttgttcgcg aatcggaata cgaggggagg aaactcagat
      421 ctccgccacc accgcggatc ggcctcgccg cccatcgtgt tatacgcccc catccattca
      481 gtgacgcggt aaatcccgtc tagcttgaac cgggcgcgca ccgtccagaa gtacacatta
      541 cagtttcgca agtctacttc aacctggtgg gccggtgcgg tcaatccagg tcggctgtag
      601 atttcctggc ccgcaagaat tacaggacca ccaagtcggc ccaaaaccga ttcgtaaatc
      661 ttcagttcat aagtaacttc actaattcgg gagatcgact cgccgctcag cgggacatca
      721 tgtgcgcggg gaaacgcttc ccatctgagc gctggccgga tgctgtcaac cttcactctc
      781 tcgaggaggc cgtatatact cggccccgaa aaatatacct tgccgcgaac ttcggggtag
      841 acagggcgca aggtgtacaa aggaaccttc ccccggatcg gcgtgttttc tttggcaaag
      901 ttctcttcgc ctggaaacgt aagcgcggat tccttaccgg tagcaacggg cgcggtaggc
      961 cggtatatga gaaatacctc gtccagtact tgctccgcaa tatccctgta tccttgctcg
     1021 aaatcctcgt caattagttt gccgccgtga gcgctccatt cctcccacgt tcgatggggg
     1081 cctcgataga tgtgggtaaa attgtccaac aacttattta ccgacaattg aaccagtcgt
     1141 acgttaacag agatctcaaa accaattaaa agctccttgg ttccagtagt tctagaagtg
     1201 atcttggtca agctgacctc aagcacggta tccatgcccg atgttccggt cgaatacgtt
     1261 gctgccgcat ccgcagaaac cggtccgagt tccggcgcca atgcgccgat tcgcaagccg
     1321 tttttctgcg catattccat cagggcttta cgcatattcc cctgaatatt cacatcagcc
     1381 agcctcgctt tgaggctgga ttcggcggag tcaattgtct cgcttggcgc agctcgcctg
     1441 tcggcggaga tcgcaccgat tgtcgcgccg atcggcaggc acgctaaaaa caccaatgca
     1501 gcagcccctg cagcagcgcc ggatgcgcta ccgcgcagga gttcaaagca ggtgcctgcg
     1561 ccggacaatg ctcctttgcc ggctgcatcg cctctaccgc gaattaacgc gtcaaagtca
     1621 atcgtgggct ggaactggcc gacggcgaga ccaatcgtgc caagcggtgc gcggtcatat
     1681 tgctctgctg gaatcttgtc ctgcgcggtt gcaattgcag gctgcgcgaa aatcaggacc
     1741 gcacaggagg cgaccgcaat cacccgggtt attcgagaat gcctcattag ctttccctca
     1801 atagccgagt ccgacgcgac gcaagaatga cactggctgt caacgaggtt cttgttatta
     1861 atatagcaaa tatcaatccc aaaccaagcc attcaggtca gagcgactat tacccaatgt
     1921 gcgtgatgtc gctccgacct gaatgtgtgg acacgcggcc tagctgccga tgtgcacctg
     1981 gtactgcgcg ccgttggaga acgtgcaggt gccggcgccc aggaaggcgc tgctcatctg
     2041 gtactcgcag cgcgcgaagg tgccgcgggg accgtaggcg ctcgccacgc cgcgccgttc
     2101 gtcgttggcg attcgggtcg cttcaccgac cagcgtctcg ccctggtagc tgaacgtgaa
     2161 gcggcccttg ccggtcatca tgttcgtgac ctggccggtc aacacgccgg tctgattggc
     2221 gagatcgttg atgggataga gcttcaccgg aagcgtcgcc ggcatcggcc cgcccgggac
     2281 cgcaacgggt tgaggtccct gggtgtggcc tggcgcgggt acgacgggcg ggatgggcaa
     2341 tggcgagtac acaaagtacg gattgccgct cgagtcctgt ccgaccggca tgacgtaaca
     2401 accggaaagc gcgagcaccg gcagggacag taccaaagcc ttgcgcagaa gcgagtatcg
     2461 agagaagtta cggagttcca tgttgcctcc tttcacgatt gatgagatgt ttccgttctt
     2521 gcgcgaaccc ttgattcatt cgcgtagccg atgcgccgga accttcactg cggcgctcag
     2581 tgccagcgaa catcgccgga ccgcgagtcg agctcgaaca tttctcgcag gtattcctgg
     2641 agggccggca tgtcgcgctg gtgtgtcagc gcgaaccgct cgttgcacag cgcgtcctcc
     2701 tcgtcgttga agacgaattc gagtatttcg tccgcgcgcc ggtcatgtgg cagttcacgg
     2761 gcctggctgc gccggctttc agtcttggtc gtcatatgcc gtctccgtct ttggtttgcc
     2821 gcgtggttgt cctgcggcta tggggcacat gctgcgacgc gcgcgcatgc agatcgctta
     2881 ccgatcggtt acagatcgat tacttttgcg gtgactggta atacacacgt gaaatcaacg
     2941 tgttggaagg aaggtctcgt gtgctacgga ctaccggctg aaggttcatt tggaggccgc
     3001 ccggctgttt cggccaggga agtcgccaca tccggcgcgg cgctcaagcc gctgatcgcc
     3061 ggatagagcg ccgaatgctg caggccgggg tggcgcttgc cgtagcggtc cggcacgtcc
     3121 tgggtccagt cgctcggtat gacgagtgaa aaggcgacga gcgtcgcaag tactgccagg
     3181 cgccagccgg tccgatttgt cccccgtttc ggtgacggag tgttctcttc gccccggatg
     3241 cctcgcgaca cgctgctttg ccgtgtgatg caccaagcaa gcaaagccag cccggcgagg
     3301 ctcatggcga tgttcagaat ctgcccggtc gcaagcccga gcaaggtcgt gggatattcc
     3361 ctgaaaaggt ccacaacgat tctcaggccg gcatagccga acagaaacaa tccggtgacg
     3421 acgccgggag ccggtcgttg ccggtagacg aggagcaata ccggaacgag cagcagattc
     3481 ttgatcgcgt cgtacagcac gacgggatgg cggaacccct cggcatccgg aaacttgacc
     3541 gcccaccatg catcggtggg gctgcccacg atcaggccgt cgatgaagtt gccgatccgc
     3601 ccgaacccca ggatgaaagc ggcgggtacc gccagcgcgt cggcagtaac caggaaggac
     3661 tgccgatgga tccggcaaaa gagcaggatg cccgccagcg cgccgagcag cagtccgtgt
     3721 gtggccatgc cgcccagcca gtacgccggg atcaggtgcg gatggccgcg gtaaaacggc
     3781 cattcgtaga acaccacctc gacaagtctt ccgccgatga gaacgccggc cgcaaccagg
     3841 atggacaggt cgtaaaccct gccggcggtt agcccgagct cccggcgccg gctccacagg
     3901 aatatgtaga tcgccagaaa tcccagggcg taggcgaggc cgtaccacca caggtggagc
     3961 ccgaagagag atccaatgac gggatcaatg tcgtgaacgt aaggtccgat catgtgtcat
     4021 ctcgcattca tcgccggcgt gctttcagga caggttttgg taggcgtgga aggtcagcca
     4081 gcccagcaac gcccccgccg gaaggtcgag cacgtagtgc tgcttggtga acaggcagga
     4141 cagcgcgatc aggacgggga aagcgaatgc aagcgcgccg agatggggca gaaggtgcag
     4201 cgcggtaagc attgccaccg acgtgtgcat gctggggaag ctgttggagc gcgcatcgaa
     4261 gcgctggacg aatgccagga agcgctcgga cagcgtgcgc tgctggttgt aggatcgcca
     4321 ctgttccggg gtcgtgacgg ggaacagcac gaagaaggac atttgcagcc cgagcagcaa
     4381 cacgtagctc atcgcgacat aggtgaattg cctgggcgac tccagcacga agttcaggta
     4441 gaggatgagc gggtagtaga tgcagctgta gatccacacc cagctcggcc agtacgggat
     4501 ccactcgtcg agccgcaatt tcagctcgcg cggcgtcgca agatagttgc gctggcacca
     4561 gaagtagaac tggtagacgc cgacgatcag gatcacgctc agtaccagat tgatcccgta
     4621 atcgatgagt ttcatgggtc gttcccttct ttttctatcg gaaagcggcc gggagcgcgg
     4681 tgctcagcca gggcaggtag gtgacggtca gcacgccgaa cgcgagaacc ggaagcaggg
     4741 gcgcgacgct cctgcacacc tcggcgatcg acttgttgaa gcgggaagag gcgtagaaca
     4801 ggttgacccc gaccggcggc gtgagatagc cgagctcgag gttgacgaga atgatcacgc
     4861 cgagatgcac cgggtccacg ccgaaagcga gcccgatcgg caccaccagc ggcgcgacga
     4921 cgacgatcgc cgagaaaatg tccatgaggc agcccaccgc gagcagcagg acgttcaacg
     4981 ccagcaggaa cagccacttc gactggatcg tctgcgtcgc ccaggcgacg gcgcgttccg
     5041 gcagctggat gtccaccagg aagttggtga gtcccatggc gaccccgagg atcagcagca
     5101 cgccgccgac aagcgccccg cattcggtcg tcaccgcgag caggcgtcgg gactcgcgca
     5161 gatcgccatg caccagcgtc tcgacgatga acgcgtagag cgcggtcagc gccgccgcct
     5221 cgaccggcgt ggcgaatccg ccgagcaggc ccgcgagggc cacgaccggc agcgcgagct
     5281 cccacttggc ggcgtacagc gcccgcttgg cctcgtccca gtcgaaggga gcaatatcct
     5341 gccggcttcg cgggtcgcgc cgcgcgcccc accacgcggc gagcacgatc atgagcaacc
     5401 ccggcagtag cgcgccgagg aacatccgct cgatgctgac cttggcgacg atcgcgtaca
     5461 gcaccagcgg cagacacggc ggcagcaggg cgccgagaga acccgagccc gtgacgaggc
     5521 cgagcgcgtc gcgctcgcgg taacgcgccg aaaccaggag cggcagcagc agaccgccca
     5581 gcgcgaggat ggtcaccccc gaggcgccgg tgaaagccgt gaagaacgcg cccacggtga
     5641 cggccgcgat cgccacgccg ccccggaaat ggccgaacag cgcctcgaac acccgcgtca
     5701 gccgcgcggg cgcgcccccc gcggcgagta tgaagcccgc cagggtgaac aggggaaccg
     5761 cgggcagcga gggattgacc accagacggt agtgatcgag tgacaccgac gcgatgggcg
     5821 cgccttgctg cgagaatagg aacagagagg cgcccgcgag aaccgcaaag ataggggttc
     5881 cggtgagcgt ggacgcaacc agcagcaaca gcgcgggata gaaccacaag gccgggtggg
     5941 cggcccacag cggcgcgcag gcgagcaggg caacggtcag gcacaccgat aacacgcgcg
     6001 cgtatatgcc ctcagaggcg cgccaggcga tgtgcagcgc gatcagcgcg aagcccgcgg
     6061 gcaggaccga ctgcgccgcc cagaccggaa tctggtatgc caggacgttg ccggcggctc
     6121 gttccgccgc gacgagctgg aggctcgcga tgcagagcag gccgcaaact gcggcggcca
     6181 gcgtgcggct cgcgaagtgt ccgatccgct gcgcgtgcgc gccgaagagc gcgctgacat
     6241 cgaacagcgt aagcagcctg ccgtcgcgcg cggcaaccgc cgcgccgatc atgctcgcga
     6301 ccagggtcag gtgctggacc agtgaggcgc tgctggtgat gcccgcgttc agggtcttgc
     6361 gcagcaccgc ttcggcgagg ggcagcagca cgatcgcggc cagcgccccc gcgaggagcg
     6421 agttctcgcc gattccgagc caggcgcgca ggcggacggg agcgctgctg ttcgtgcccg
     6481 tggcgactgg agccatcgcg gtgctcatcg cgccgctccg ttgctcgttg tgccgcggtg
     6541 ctccgcgagc gcctcgtgca ccgcgtcgaa catgtcggcg ggcaccattg cgccgcgaac
     6601 cttcggatag acggattcag cgagctggcg ccactcggtc tccagctccg gggtgagagg
     6661 gtggacctgc aggccgcgct ttttcatcgc ctccacggct tccgcatccg cggtcgcgcg
     6721 ctgagcccgc aactgttcgg ccgccttctc cgcgccgcgc cgcaacgctt gcttgcccga
     6781 ggggctgagc gcgtcccatg cgctgcgcgt gatgaccgcg gcgccgacaa cgggcaccca
     6841 gcggatgtcc agcatatgcg gcgccgtcac gtcgatctgg cccgccagcg cccaggcggc
     6901 cgtgaccggg accgcgttga tgaggcccgt ctgcaggccg ggcaggatgt cggtggtttc
     6961 gagcacgacg ggctgatagc cgagcgcctt catctgggcg atctgttccg cgtcgccggt
     7021 ccaggcgaac atcttcagcc gcctgaaatc ggccgggcgc agcgcgcgct ccttggaaaa
     7081 aaagcgcacc cagccgcctt cggcccagaa cagcacgacg aatcccttgt cgagaaagcg
     7141 cttctcgagc agcggtcgca cccgctgtcc gaccgcgtcc acttcctccc acgaccggaa
     7201 catgagcggc atcttctgca gcgctcccgc gccgggatcg atctcggtca ggccgatgac
     7261 ggagatgagc gcggcgttca actggccgat gcgcatgcgc cgcacgatgt cggcctctcc
     7321 gccttgcgag ccgtccggaa agacggtgaa gctcgcgccc ggcgcctcgg cgcgccgcca
     7381 ggtttcgccc atttccatga gcgacttgtg gtagatcgag cccctgggcg cgatcgtgcc
     7441 gagcttgatg cgcgcggcgt cctgcgcggc tgcggctgcc gtgacgcccg gcagcaggca
     7501 cagcgccgtg atcagaccga aatgccgcat gaatttgctg attgccatgc ttgttcctcc
     7561 ggacaggtct attcggaaaa aaaccggtcg gcccgcgcga gcagccaccg tgcgcgccgc
     7621 tgcatgacga gattgacgag acgccactcc ttgttgccgg aggcatccag ttcgagggcg
     7681 tgttgcagca ggctttcgaa ttcgccgcgg ttctgctccg ggatgctgac ggcctcggcg
     7741 agcgtgacgt aaggcgccgc atgccggcct ccggtcagct cgactgcccg cgcaaaatgc
     7801 gctcgcgccc tggcggacgc gccggctctc aagccggcgc ggctcatctc gtaattgatc
     7861 atgaatacgt ggatggcgcc gtgatcgtgg gtgtcgtcga gcgcgagcgc gtgctggacc
     7921 agggcctcca ccgccggcaa atccgcgatc atgaaagggt cgtctttcga cagggaaatc
     7981 gccgcggccc acgcgacacc ggtccagtag agcaatccga catccgcctc ggatgttttc
     8041 gatacggcgt ccagcgggcg ggtcttcagg gtcgtggtga agcccgggtg cgctgcctcg
     8101 agtccgcgga gcccgtaatc gcgggcgcgc aggtacatgc ggcgcgcgcg ctcgcgctgg
     8161 aagtgagcgg cctgcaggtt cgtgtcctcg atctcatcgg ccttcaactc cacgtaggcg
     8221 tatgcgtact ggacgaagcc ctgcgctgtc gccagcagca gcgcgcggtg gcgggggttt
     8281 tccgccagca tgctttccat caacttcagg ctgaacggcg ccgcggcgcc gatgagctcg
     8341 acgtcgtcat cggaaccgaa gccggagccg ctggccgcga gcgcgtcgcc gacgcggctc
     8401 atggcgtagt tgcggatgga gcagccgctc gcaccaaggc acagcgccag ggcgactgac
     8461 agcgcccaac ccttccaggc gggtgcgccg caagcgatcc ctgagctcaa ggtgccgttt
     8521 cgagtcatgg atgtcctccc tcagtcaggg gcccatcgtc gcgcgacgat ggtttcaaaa
     8581 ggcttaccgg tgacttacga attacttaaa gatggcgcta tggcgcgtga ggccggactc
     8641 gcctgtggcg cggtggcgcc ggccgttgcg caagcaacgc ttccgaaaat gctcgcggcg
     8701 tttgcgcgtt gcttcttcgc tgtaaagcgc tacgacgagg cgcttgagcc ctgcgcgcag
     8761 gtcctcggct gtcattcctc tcggccggaa gttgacgtcg aagagcgtgc agcgctccca
     8821 cgcttcgtca ccgaacaaac ggccttcggc cttgagttgt gcgtagaacg gagtgcctgg
     8881 aaacggggtg ggcacggtga cttgcacgtc gaaggggcac gcatctcgca cgaaagcgag
     8941 cagggcgtcg aacaccgaag ggtcgtgacc gtccagcccg acaacaaaac aggcattgac
     9001 acgtatcccc gcggcctgaa tgcgctcgat ggcgcgcaag tactgaccat agcgctcgtg
     9061 tttccagttc cgccgcagct cgagaccgtc gagtccctgc accatagggc tttcgaaacc
     9121 gatcagcact tcagcgcagc ccgccctgcg catcatgtgc agcagctcct cgtcctcatg
     9181 gacagcaagg tcgctctcgg tgaaccagcg gatgcggcgc ttctcgagtt cggggagcag
     9241 ctcgcgccag tagcggcggt tgacgaacga gttgtcgtcc gcgaactcga tgaacgggcg
     9301 cggccacaac gcgcggatac ggtcgatctc ggcgaggacc ttgccgaccg gcttttgttt
     9361 gtagcggcgc gtgagcagga tcgagctggc gcagaagctg cagcgccaag ggcagccgcg
     9421 gctcgtctga accgtgatgc ggttgtagcg ttgcaggtcg agcagcccga aagccggcat
     9481 cggcgcgttg gccaggtcga actccctgcc gtgcgcgtcg tacaccggct tcaactggcc
     9541 gcgctcggca tcctccagga tctcgggcca gacgatctct ccctcgccca gcacggcgct
     9601 cgcgcccgag cgctgcggct cctgggggtt catcgtgacg tgcaggccgc ccatgacgac
     9661 cggaacgccg agcgcctgat aacggcgggc aagctgatag ccttcgttga tctgggcgct
     9721 gaagctggag atcgccacca gatcgaagtc cgcaggcagt gattccatcc gcttgaggtc
     9781 ggcaacctcg atataacgca attcgtgccg tcccggcgtc atgcctgcga gggtgagcag
     9841 ccccaaactc ggaagcgagg cgatcgcctt cgagcgttcg acgaatcccg gcagcgtgag
     9901 gccaagcgca agcagttcct cgtcacacgc gcggatgccg ctcatcgcga tcaacccgat
     9961 cctcatgaca ggcctcccgt gatgaacgtc agcgccgccc cgaggagggc caacaccggc
    10021 agcgggaaga gatgacgaag gggtttcttc caggccgcga gatagcagac gagcggcatc
    10081 agcatcgcgc cggcgacgag cagcatcgac gccagcggca cgtggctcgc gggaccgagc
    10141 agccacacgg tgttggcata cgcgagattg aggaatgcga cgccgaagaa gctgatatgc
    10201 ccgagccgca gcaggcgccg cggccacgag ctgtagcctc cgagccagtc atcgcggtcg
    10261 aagaacaatc cctggaacat gccgcttgcc atgccgagca ggatgccgat ccacgccgcc
    10321 caaagattaa gtccgctcat gcctcgttgc ctccttcacg ctccggccga tgaacggaat
    10381 cattccggcc aggcgttccc atcgtccggc aaccgaagtt gcagatggct tacgcgcgac
    10441 ttacctatcg cttaaagatg cgcattcgcc acgcaatcag acacttgcta taaggcggat
    10501 ggcggaacgc agaagagagg agacaggaag cggaaggcgg agttgatgtt ttacgccctg
    10561 cgcatgaggc aaccaccaaa ggagccgcgc aatgcgcgta tcgcccccac cgcggcttgg
    10621 ataggcggca cattcacggg agcgcgcgtg cacgcccggc caccagttac cggcaaccag
    10681 atcgtggcgt cgcgataggg caactactgc gcgtattcga tgatcaacgt gtgatccggg
    10741 accgcgacgc cctcgcccga gagcagtccg gtctgaccgc gccggagcgc gccttcgaag
    10801 tcgttcacga aaaagccgcc gctcgagagg ttggggaaga agatgctgat caccgaatcc
    10861 cgcgttgctg tttggactct tgcccaagag cgctcaccgg tgccttggtt catccgcaat
    10921 gcgccgccga ccgtcagatt gatgtcaccg ttgctgaaga gcagggcgta tgcatcttgt
    10981 ccgctgccac ccatcagact gaggtcgcca ccggtgctga cactgacggg tccgtttccg
    11041 gtgatggacg cgctgccgcc gcttgtggca ccgccttgaa caaggagcgt gccgccggcg
    11101 tttagcatct gtggacccgc tgcgctcagg gttgtgctgc tgccggcaac cacggtgata
    11161 tcgccggtgg cgctgatcga ctgcggtccg gaaacgacgt tgcctgtaat cgcgagatcg
    11221 ccagtctgat tgagcgtgag cgccccgact ggagcttgac tgatgccggt cactgttagt
    11281 agcggtgacg tattgttgag cgcgacgtta cccgaaacgc tcgacgcatt gaatgcgctc
    11341 accaggttcg cgccggtaag gcttgtattg ccgtttgacg tggtaatgag cgcgttcgcc
    11401 tggaccgcgc cattcggatc ctcgctgatg gtcccgcccg ggctgtccgc gtgtagctga
    11461 acattgccta gagtacgtat cgtggccgcg cgcgtattgc cgtttacctg gatggcattg
    11521 cctgcagtga tcgaaatatc gcctccggtc gctggtgcgg ggctgccgat tcgcgcgccg
    11581 gcacccgtgc ccccgctcag gacgacgttg cccccggcgt tgatcgcaat ttgagtgagt
    11641 tccagtggtc ccgcgcccgc gctgccgatt acggccacag tgttcgaggc tgcgttgccg
    11701 gtcagggcaa cgttgccacc ggcgcttaga gttaaatcgg ttggcccggg gtttgccccg
    11761 ccccgacccc cgatcctggc gctgtttccg cttccgaaac cgccgcccag cgtcacgtta
    11821 cccatggtgg agatatcctg ggtcgcgccg aaaataacgg ccgcgttgtt tgtgccgcca
    11881 gcgcctccca gcaacgagat gccgtctgcg ccgacggtga tctgttgcgc gccattgagg
    11941 gcaccgattt gcgcgttgtt gccagatccg ccgggcccgc cctgcagagc gatcgcgccg
    12001 gcgttgatcg tctgctgccc gccgatgcca ttggcgaata tgcccgcggc ggacgtgccc
    12061 gccggcgagg tgccgccgat caccgtcagt gtgccggggg tgctcacggt ctggatgccg
    12121 cccggaaccg cattcgacac gatgatcgag ttgccgccgc taccggctgc cttgccgatt
    12181 atcgtgatcg atccttgctc tccggccaac ccggcggtga cggtctgcgt gccgccgccg
    12241 ccgatttgcg aagctccgag cgagcccggg ctgccgaggt tgagcgcgtt cgcgctcgcc
    12301 gcgccggtga tcgagatggc ctggattccg ctgttgtcaa aaatcaacgc attgccgccg
    12361 atcccgtcaa cggtcatacg ctcggcgttg ttcacgctga tctgctgccg cccgcctgta
    12421 acattttgcg caatggtcgc aaacgcgcca gggccgccga gattgcgtaa agcaagccct
    12481 tcgccggacg cattcatgcc ggtcgtcgtg atgagctgat caccgctcag gttgttgctg
    12541 acaaacgccg gattgtcttg cgccgttacc tcgatatacc tggcattgat cgtttggccg
    12601 ccgctggcga gtagctgcgc ggcgccggca gcgctgctct gcacgacaag tccttcgccc
    12661 gccgtgatcg attgcggccc gaatgcattt acgaacgcag ggctgccggg aagcaccgag
    12721 atgctgccgg tggcgttgaa agtctgcgga ccggagctga tcgtgccggt cagggtcagg
    12781 tcgccggcct gattgatggt gagcgcgcca cttgaggtct ggttgacgcc cgtgaccgta
    12841 agtagcggag tggcattgtt tagtgcaaca tcgccactcg tcgtattggt cgcgttgaag
    12901 ctcgcgacct gattgtcgcc agtgaggctg gcgccgttcg cggtgttagt ggcaagcgac
    12961 cccgcctgaa ttctggcatt cgggccctgc acgatcgagt ctgcctgcag agcgatgctg
    13021 tcggtcgtgc ggatgacgct tcccacgagg ccaggcgcgc tgttgaggaa gagatcgccc
    13081 cccgcggtca cagagatatt tccgccggcc ggtgcaccgc tgggagagcc gatccgcgaa
    13141 ccggcattcg ccacggtacc gggattgagc gtgacgttgc cgccgacaat catcgcgatg
    13201 tcggtgggct gaccgccctg gaaagcactg ccgataacgg ctccggcact ggcctcggtg
    13261 ccgccgctcg tggtgaagtt cccgtccacg tttaacacca gattggtcgg tcccggaatt
    13321 gcgcccccga ttccgccaat gcgtgcggct ccggcgctgg ttggaccgct gaccgagttt
    13381 ccgcccgtaa gcgtgagatc gccgtgaata gtgatgtctt gatccgggct gtggataccg
    13441 gcgaaattgc tgaacccggc gcctcccgct gcaagcatcg cgctccccgc agtgatgttc
    13501 tgagatccga tggcccgaat ctgggcaaaa ttgcccaggt cgggcgcgcc gccggtgccc
    13561 ccggtgagac ccaggggccc ggtcacgctg accgtctgcg cgcctatccc atcgttgcgg
    13621 atgcgggcga agttatccgt gccgcttgcg cctccggtga tcgtgatggt gtccgacgca
    13681 ttgatcgact ggcccgccgc cgctcccaac tgacgaatct cggcgaagct ggagttgccc
    13741 acgctgccgc cggtcacgtt gatgctggaa gcgctgatcg tctgtcctgc gctgccgagt
    13801 atctgagccg accgacccgt tcccgcgccc ccttgcacgg taagcgtgcc gccggcggtg
    13861 atggtctgcg gacccgtggc ggttacgatc gcgttggtgg cattgggcgc tgccaccgtg
    13921 atgtcatccg ccgcgttgat cgtctgcggc cccgagatga cgctaccccc gacggtaatg
    13981 ttgttcgcat tcaacgtcag gctgtcgagc gtgcggatga cagaacgcgt gccgctctgg
    14041 ttggtggcgt tctcgagcgt gatgtcgcca ccggcggtga tcgagatact tccgcccgca
    14101 gagcttgcgc tcggagaccc gatgcgcgcc gaggaattgg cgctggtgcc ggtgccgagg
    14161 gtcaggttgc cgcccacgtt catcgtgatg ttggtaggct ggccgccgag gaaatgactg
    14221 ccgatgacag gaccggcaaa ggccatgctg ccgccattca tggtgacgtc gccgcccacg
    14281 gtgagcgtga ggttggtcgg cgttgccgga ttgggtctgc cgcctatgcg cgccgcgcca
    14341 tcccgcaagg gaccgctggc cgacgcgccg ccggtgagcg tcagatcgcc ccgcacggtg
    14401 atgacttgaa cgggagcgtt gatacccgcg aaattgttga agcccgcgcc gcccgccgta
    14461 atgtctatgc ttgccgcggt gatgtcctgc ggtccggtgc tgaaaatgct ggcgctgttg
    14521 ccctgatcca cgatcccgcc ggtcccgcca gtaatcgtga tgatcggtga gcccgtgatc
    14581 gtctgaggcc cggtgctatt gttctggatg cgcgcgacgt tgctgctgcc gctcgccccc
    14641 ccggtgagcg tgatttcgga agcgctgacg gtctgggcac cgctggccga gaagaattgc
    14701 gcgctgttac cacttccggt caacccaccc tgaagcgtga tgcgggccgc cgtgaccgtc
    14761 tgcggcccat ttgagttgtg gttgataccg gatgtgaaag tctgtgctgg cgcgttgccg
    14821 cccgtgactg tgagggcgcc gcttgtggac accgtctgcg tgcctgggaa gcccacgttg
    14881 gtcgagatag cggcgaggcg ggaattgacc gcaggtccga ctatgttgat ggaaccagac
    14941 tgtccgctcg tgcccgccgt gatgctctgg ctgcgcgcgt tgatattcga gccgcccagc
    15001 gcaccgacgc tgccgacatt gattgcgttg gcgccgctgc cggtgatcga gatcgtctgc
    15061 gtgccgggaa ttgcgtgaat gatcgcgctg acgttggaga tgtttggtcc gaagccgttg
    15121 acattgatgt gatcggcgtc ggtcacgttg atcacttgtg ccgctcccgg cgcgttgtta
    15181 cctatggaag cactaccgcc gctggccagc gaccgaacgt cgatccccgg gctcaaaccg
    15241 ccacccgcca cggtgatgac ctgatccccg ctcgtggaat tgaagattcc cgcggcgttg
    15301 ttgtcttgcg ccgttacttc aatgaagcgc gcattgatcg tctggccgcc ggtggcgctt
    15361 agctgcgcgg ggcccgcggc gctgctctgg acgatgagcc cgctgccggc ggtgatggtc
    15421 tggggaccgg aagtgatgtt ggaggcgaca gtgatatctc ccgcctcgag ttcgaggggt
    15481 cccgtagccg tggtaacgct gcctggcgcg aaggttgcgc tgttcgtcgc cttgagaacg
    15541 atcctgccgt cctcggtcgc gaccgcggtg ttggcttgga ttctgccgct ctgattgatc
    15601 agccccgcgt agattcccac tcgccctgaa tcggcgatga tctgcccgag attcagcgcc
    15661 tgattgtcgg gggccacgat ttcgaccctg agattcggcg tgcccgcatc gacgagctcg
    15721 acggaatttc ccgccgcgag gattacttcc ccctgtgggc tggtgatgat cccgctgttg
    15781 gtcactgctg gcccgaccag atacacctgg ccgcccgcgg gtgtcgtgat gtttccctga
    15841 ttcacgaccg aacctgcacc cggaacttcc gtgaatctca ttcgccccgc caggaagtcg
    15901 gcgtccgaga gactaagggt cgaggcaacc aggcctgcca cgtcaatttg agaaccggcg
    15961 ccgaacacga tgccatttgg attgatcagg aacacgcgcc cgttggattg cagcgcgccc
    16021 aggatggttg acggattctg agtgatcacg cgattcaaga ccgcactcgc ggacgactgc
    16081 tggatgaagc gcgtcagctc gccggcgccg atcgagaagc tctgccagtt gatgatcgaa
    16141 ttgggcgagt tggtgatctg cagcacgttc cccatcggta cgacgctcac ggtgccgtta
    16201 accacggtgg ggccggtcgg gttggcgaac gcgagggagg aagcgaagca ggatgtcact
    16261 gccaccgaaa tcagtttcat ctgacgagtt ttcgctgcgc gattgtggcg aatcttcatt
    16321 ggagctcctc ctggtatttc gtcggttttt tgccgccaac gcattacgcg aagatcacga
    16381 ccgtgcgagg ccgactgcgc gcaatcatgc gggatagatg gcttactgga catcccatgt
    16441 acatgggatc acgtgcgcgg gcggtagcag ttacgataat cggacgatgc aagaaagccg
    16501 gtaagaaaac aaacggttgg agcaggaagc cctattattt atgccgattc ggaatgaacg
    16561 cacacgcgag cgtatgctac tgacatgagc aaaaggaccg gcgcactggt acgcgtcatg
    16621 atcgtcgagg acgatccggc cacactcgaa cgcttcgccg gagcgatctc gcgcgacccg
    16681 cgtacccggg tcgtggagaa ggctcgcacc ggcagcgagg cgatcgcgcg cttggtctcg
    16741 gtgcgtcccg acgtgctgct ggttgatctg ggcctgcccg acatgcatgg aacgcgcgta
    16801 atcagccacg cagtacgcgt gcatcccgcc tgcgacgtca tggtgattac ggtgttcggc
    16861 gacgagcgta acgtgctcgc cagcattgag gcgggcgcca ccggctacgt gctgaaggac
    16921 tgcagcgatg atgagttgat tgccaaggtg ctcgagttga gaacgggcgg cgcaccgatg
    16981 agcccgggca tcgcgcgcct cgtgttgagc cgcatgcacc gacaagaccg gtcccggtcg
    17041 aaacagacgg ctgccggagg cgaacatgcg attctgacgg agcgcgaggt ggaggtgctg
    17101 agcctcatcg cacgcggcta tctttatgca gaaatcgcca agcgcctcgg gatctcactc
    17161 aacaccgtga ccagccatat caagaacagt tatcgcaagc ttgcggttca ttccggggcg
    17221 gcggcagtga cgcgtgctgc ggagttgggc ctgctgcgct cggatgacga caaggggacg
    17281 gcgcgttgag cgccggcgca gccggccatt acacgtggaa tcaagagtct tacattggtt
    17341 cccatgcccg gttgtgattg aatcgtcagc cgcgcgccga tgcgctgcgc ccgggcacgc
    17401 atgttggcga ggcccagccc ggcggagaat tgcgaggggt tgaagccgca accatcatct
    17461 tcgatgcgaa tctcgacgtc cgcatcgcgt ccgcgggcgg tgacacgcaa gtgtcgcgcg
    17521 ccggagtgtt tcagtgcatt ggttatcgcc tcgagcaacg tgcgctgcaa ggcgaacacg
    17581 tcggccggct tcaacgcccc caagtcgggc agttcctcga cgtcccaggc aaaactcacg
    17641 ccggacaggc ggatcatgcc gtcgagccga tagcgcaggc tgccgagcac cgccgcgagg
    17701 tcgccctccc acggctgcag ggcgtcgatc gcaatgcgca tttcctgcaa cgcttcctgg
    17761 acgcgccgct cgatgctcgc gcgatcagcg tccgcggatt gcacgtgacg cagcagcccg
    17821 acaaggctcg ccccgagccc gtcgtgcatg tcggcgagga tgcgctggcg ctcgcgcgcg
    17881 atcgcctgtt cgcgccttgc ctcctcgacg cacgcgtggt cggcctcgat ctcccgcgct
    17941 ttctccgtga ccctgcgctt gagctcgcgc atcgttaacc gatgtcgctc gattgtcgcg
    18001 atgcccagag gaagaaccga caacgcctgc ccgcccacca gtattgctat ccagatccag
    18061 ggaaaccact cgcggtacca gagcgtaggc ctgctcgaag gcgcctcttc gctcagcaac
    18121 caccatccgg cggggacgag taccagcagc acggtcgagt agaagatggt cacggtgacg
    18181 ccccaaccgt acaggaagga gccgggttct cgccccacca gctgcgtgat cgctacgcgc
    18241 atcatgggca cgggcattta cttcgtgcct agtgttcatc tggacggaag gccaagtcgg
    18301 cggaccagcc agaacagggg aaggatcaga aaagaaccgg gtatcagcag caccagcgcg
    18361 tatacgcacc actttacgtg ggggggcgct gtgggagcat cgagcgtcgg tgccaacagt
    18421 g
//
LOCUS       DBNGWZ010000512         8959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_695109, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000512 DBNGWZ010000000
VERSION     DBNGWZ010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_695109"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16CX_15655"
     CDS             complement(<1..217)
                     /locus_tag="AC16CX_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3013978.1"
                     /translation="MPVVESNKLFPVGRIYCVGRNYAEHAREMGHDPEREPPFFFMKP
                     ADAIVPNGATLPFPRATKDLHHEIEMVV"
     gene            complement(350..901)
                     /locus_tag="AC16CX_15660"
     CDS             complement(350..901)
                     /locus_tag="AC16CX_15660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013979.1"
                     /translation="MSQTITQPSSLISEEYRLMQQKLHENPDYGVASIGYAPLVAQIV
                     EQVGATELLDYGAGKGRLGIALREHLRRPLTIHHYDPAVPEWSAPPAPCRFVACIDVL
                     EHVEPTLIDNVLDDLKRVTAGVGVFTVHTGQAAKFLSDGRNAHLIQKPPAWWLPKILE
                     RFELVTFNRMPQGFWVGVERKMA"
     gene            1065..2159
                     /locus_tag="AC16CX_15665"
     CDS             1065..2159
                     /locus_tag="AC16CX_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3013980.1"
                     /translation="MKIRSIKAGVHRFAVTVPLLKEPIQRNAVFCEVETDDGYAGHGL
                     TGGAYLPLAIVTALEKEFLPVVKDMDPRDSEAIHEKVWWKINQRSMTGVVSSALSVLD
                     IACWDIHGKLTDRTVAQLLGGARDWAPTYCTFGFPEYDRDQLVEAARLQVKNGHRRLK
                     MVVGVHKDGWKEDARRVRAVRDAVGPDIELMMDANYKFNPVDARQLCREIEDCKLTWF
                     EEPLYQNDTRAMADLRAHTSIPISAGQNEGHRWRLRELVLHQAVDILQPNVCYCGGYT
                     EAQKVGHLAQAFNLPIANGGGWPLHNMHTMAGLMNGWRVEFHLDMQAVGEMIFINPPR
                     PENNIVKIPARPGLGLEPNRDVLKDTLVKS"
     gene            2360..2755
                     /locus_tag="AC16CX_15670"
     CDS             2360..2755
                     /locus_tag="AC16CX_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HIR3013981.1"
                     /translation="MSEEASLWVLFSSSFLAATLLPGGSEAVLFGVLKLHPGEVWPAL
                     GVATLGNTLGGMSSYLVGRIIPPKKDLPGLPAVQKYGSPVLLLSWVPILGDPLCVAAG
                     WLRLNPWLSTLFIALGKLGRYIAIAVIAA"
     gene            3079..4674
                     /locus_tag="AC16CX_15675"
     CDS             3079..4674
                     /locus_tag="AC16CX_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013982.1"
                     /translation="MPAKASGAAGAQKQLQFSDADGCRAWVARLPLTNPAQCHALLAA
                     QVRLLPAATIAAAAKLEILELLRQPVEEAQSEMMKGCRGQPVPLEAKDRQAWESVVGT
                     WRAMAAGYDALIDAMASTAPDLATHAHLICQRALCYTAAAMFECCHIYHAVSGDLWKQ
                     LHRLYVFSENAGLATTTVDDAIGRRTATTSCLATYVHVLLLHLAQPDALSVQQLDTVH
                     RWLERWDGLVTLSPEPLAQSAIPALAVDMTSQKGAGLARQMPAAGVRHLNLEALSKTL
                     RQTAAALKQQTPAQLGLGDLPREACEKLLLLLQVQWCAAGTGRVDERTPASIKVMISP
                     NIASMHFHLTGKAFRRPVGEITAAERQRLDMLGSVSEAGEQALASQRSAALETWVILN
                     QSVSGFLGTRRKEEPGSRICYYQLVALQPAARKVMYVGIVQRLIVDEAGTLWIGLRII
                     LGAPQAVTARIVEGAGKYDRALLMPEDAAHKIPASIVLLPGWYQANRTLGLHTDKEKQ
                     RRVKLRALLDKGVNFERATYIAA"
     gene            4756..5835
                     /locus_tag="AC16CX_15680"
     CDS             4756..5835
                     /locus_tag="AC16CX_15680"
                     /inference="COORDINATES: protein motif:HMM:NF022447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HIR3013983.1"
                     /translation="MNPELETFFSPDYVSARERFRAAALAAGAALDALAIEARGPRQE
                     PLSIDIARLGERDARRVLLHTCGLHGVEAFAGSAVQLAALEDPPVPPAGCALVLVHAL
                     NPYGMAWLRRANENNVDLNRNFLRRGERWEGAPALYPRLDPLLNPPSPPQPEGFRLRL
                     ALVALRYGTRAPRQAIAEGQYEFPRGLFYGGKALEPGPRLYLDWLRRNVARAVYLFAL
                     DLHTGLGRRGKETLILEPGVGATPATELTRAFGRSLIDPASGRAAYRIRGGMGGVLPE
                     ILPGAGIDFVLQEIGTSPASAVLRALREENRCHHYGAADLRHPAKRALLEALCPALAA
                     WRRRAVERGLSLLRTAAAWTFRYEP"
     gene            5914..6321
                     /locus_tag="AC16CX_15685"
     CDS             5914..6321
                     /locus_tag="AC16CX_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3013984.1"
                     /translation="MKCELCETDGGTLLWRDLQCRVVHVPEAGYPGFCRVIWNAHVGE
                     MTDLSAAERADLMRVVFKLEAALRKLLRPDKINLASLGNVTPHLHWHVIPRFRDDPHF
                     PNPIWGALIRQPGPLARTSADITAALAREMSRL"
     gene            6578..8176
                     /locus_tag="AC16CX_15690"
     CDS             6578..8176
                     /locus_tag="AC16CX_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013985.1"
                     /translation="MISTLPHSSAPAPLQFTDSTGCKRWIEQLTLTNVQLTQQVLTGQ
                     LVSLGATPLPPLERLKILETLREPVQFVQGESAKRYAGKPLPLDPGETTVWGNVITLW
                     QEMSRNYQQCLKAYREGELAIAPHAALITMRCLRLLGCTLLDHYRVYRQPPGALWRAL
                     HELHAFGESHGFARIRVQDSFSQRDPDSSCAESYVQVLLAHLVNPYSLSLRQMAFVAR
                     WLERWSTLVGLAAQSLPAGPIPSLAVNLTSTEGVVLGAGLEPQPHLRYLDLEQLSKTL
                     RQTINLLKQGQTPGQLGLGEDARQPGCENLLMLLYVQWCRAGTARVEERSKSEELAQV
                     CFGLAAAHLHVGGRSFRQPGELTAREKQDMDTYGYITRTEHEVSGKSEALEQWQILNQ
                     SASGFMCIQREPEGKVRVSHNQLLAVRRAGGHYFHLGMVQWLRMEEGGALYCGVRLFP
                     GTPQAISVRPSNFAPTGSSRYERALLLPEVPAPATPATLILPAGWFQSGRFLEIFSDR
                     KQVAKLLNLLEKGGDFDRGTIVVV"
     gene            8340..8735
                     /locus_tag="AC16CX_15695"
     CDS             8340..8735
                     /locus_tag="AC16CX_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR3013986.1"
                     /translation="MAGLNKGVPQPTEIRLHQKSRILEIAFADGKAFRLPCEFLRVYS
                     PSAEVRGHGPGQEVLQVGKRDVEITRIEPVGSYAVQFTFSDGHDTGIYSWDLLYDYGM
                     RQEEMWRHYLKRMEEAGASREPKEVSSKR"
     gene            8784..>8959
                     /locus_tag="AC16CX_15700"
     CDS             8784..>8959
                     /locus_tag="AC16CX_15700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3013987.1"
                     /translation="MTKTTDFGYQKVPEDEQPRRVAGVFDSVASRYDLMNDLMSGGLH
                     RLWKRFTVDASGMRP"
BASE COUNT         1574 a         2945 c         2902 g         1538 t
ORIGIN      
        1 ccaccaccat ctcgatctcg tggtgcaggt ccttagtggc ccggggaaaa ggcagcgtgg
       61 cgccattggg aacaatggcg tccgccggct tcatgaagaa aaacggcggc tcacgctcgg
      121 ggtcgtggcc catttcgcgc gcgtgctccg cgtaattgcg gccgacgcaa tagatgcgcc
      181 ccaccgggaa cagcttattg ctctccacga ccggcaaggt cggcgtcggc cggaagctga
      241 ccgcgtaatt cactctcttt cctttcgtcc ggtttgcgcg tcagcgaccc gctgacagga
      301 ttaccatgat ttcggtgcgc gctgcgagcg cggggcgccg tgtccgttcc tacgccatct
      361 tgcgctcgac cccgacccag aatccctggg gcatgcgatt gaaagtcacc agctcgaagc
      421 gctccaatat cttgggcagc caccacgcgg gcggcttctg gatcaaatgg gcgttccggc
      481 catccgaaag aaacttcgct gcctgtccgg tgtgaacagt gaacacgccc acgcctgcag
      541 tgacccgttt cagatcgtcg agcacgttgt caatcagagt cggctcaacg tgttccagca
      601 cgtcgatgca cgccacgaac cggcatggag cgggcggagc cgaccattcc ggaaccgccg
      661 gatcgtagtg atggatcgtc agcggccgcc ggaggtgctc gcggagcgca atgcccagcc
      721 gccctttccc cgcgccgtag tcgagtagtt cggtcgcgcc cacctgctcg acgatctgcg
      781 cgacgagcgg cgcgtagccg atggatgcca ccccatagtc cgggttctcg tgcagcttct
      841 gctgcatgag ccggtactcc tcgctgatca aggaagacgg ttgcgtgatc gtctgcgaca
      901 tgggaacttg cggaacaagc cggcggtgac cggaaaagag tagcgcatta tcttggaaaa
      961 tcctcggcgg cgtcaatttg cgaatgcggg cggtccgcgg cttaccgccg tggctcgccg
     1021 gtgtaaactc gcgcgactcg caacccgatt ccatcggagg acacatgaag atcagatcca
     1081 tcaaggccgg cgtgcaccgt ttcgccgtca ccgttccgct gttgaaggaa ccgatccagc
     1141 ggaacgcggt gttctgcgag gtcgagaccg atgacggcta cgccggtcac gggctcaccg
     1201 gcggcgccta cctgccgctc gcgatcgtca ccgcgctcga gaaggagttc ctgcccgtgg
     1261 tgaaggacat ggatccgcgc gacagcgagg cgatccacga gaaggtatgg tggaagatca
     1321 accagcgctc gatgaccggc gttgtctcga gcgcgctgtc cgtcctcgat atcgcgtgct
     1381 gggacatcca cggcaagctc accgaccgca ccgtggcgca gctgctcggc ggcgcgcgcg
     1441 actgggcgcc gacctactgc accttcggct tcccggaata cgaccgcgac cagctggtcg
     1501 aggcagcgcg gctccaggtg aagaatggcc atcgcaggct caagatggtc gtgggcgtgc
     1561 acaaggacgg ctggaaggag gatgcgcggc gggtacgcgc ggtgcgcgac gcggtggggc
     1621 cggacatcga gctcatgatg gatgcgaact acaagttcaa cccggtggac gcacgccagc
     1681 tctgccgcga aatcgaggat tgcaagctca cctggttcga ggagccgctc tatcagaacg
     1741 acacgcgcgc catggccgac ctgcgcgccc acacgagcat cccgatctcc gcgggccaga
     1801 acgaaggcca ccgctggcgg ctgcgcgagc tcgtgctgca ccaggcggtg gacatcctgc
     1861 agcccaacgt ctgctactgc ggtggttaca cggaggcgca gaaagtgggg cacctcgcgc
     1921 aggcgttcaa tctgccgatc gcgaacggcg ggggctggcc gctgcacaac atgcacacca
     1981 tggcggggct catgaacggc tggcgcgtcg agtttcacct cgacatgcag gcggtgggcg
     2041 agatgatctt catcaacccg ccgcggcccg aaaacaacat cgtgaagatt cccgccaggc
     2101 cgggactggg gctggagccc aaccgcgacg tgctcaagga cactctcgta aaatcgtaac
     2161 cacgaaaagc gagactcggg actaggggct cgaacaacaa ggaaacaaga cccggggacc
     2221 gagaccagca acttcaagcc ccgctacccg acccccgggt cccaccttgt gggcttgtcg
     2281 agtcccgagc cccgatagcc tagccccgct ttttcatccc tcatctctcc acccttatcc
     2341 ctcacgaggt tgcccggtga tgagcgaaga agccagcctg tgggtgctat tctcgtcgag
     2401 ctttctcgcc gcgacgctgc tgccgggcgg ctccgaggcg gtgttgttcg gcgtgctgaa
     2461 gctccatccc ggcgaggtct ggcccgctct cggtgtcgcc acgctgggca acacgctggg
     2521 cggaatgtcc tcctatctgg tcggccgcat cattccgccg aagaaggacc tgcccgggct
     2581 gccggccgtg cagaagtacg gcagcccggt gctgctgctg tcctgggtac cgatcctcgg
     2641 cgatccgctg tgcgtggcgg cgggatggct gcggctcaat ccctggctat ccacgctctt
     2701 catcgcgctc ggcaagctcg ggcgttacat cgcgatcgcc gtaattgcgg cgtagggtcc
     2761 acctagcccg catgtcttat gactgccgca gttttgaggt cgtgcgagcg ggtcgcgcag
     2821 gcaggcgtct gcgacatgcg ggcttagccg gtaaatttca cgcgaagctt cagggtaatg
     2881 accgcttccg tgctgcggct cggtattcgg gtcgggtcgt aatgggtgtt cagtgcgttt
     2941 ttcctgctgg cggttgctat gccggcctcg gcgttgagat aatccgctag cagactgtcg
     3001 gagcttgcta tccgacagtc tgctaaacgc aaaaccgcct gcggatttcc aggtccgaca
     3061 aattcctagg gagcaggaat gccagccaaa gcctccggcg ctgccggcgc tcagaagcag
     3121 cttcagttct cggacgcgga cggatgcagg gcgtgggtcg cgcggttgcc gcttaccaac
     3181 cccgcgcaat gccatgcgtt gctcgccgct caggtccggc tgctgccggc cgcaacgatc
     3241 gccgcggcgg cgaagctcga gatcctcgag ctgctgcggc agccggtcga ggaggcccag
     3301 tcggagatga tgaagggctg ccgcggccag ccggttccgc tcgaagccaa ggaccgccag
     3361 gcctgggaga gcgtcgtcgg gacctggcgc gcgatggcgg caggctacga cgcactgatc
     3421 gacgccatgg ccagcaccgc cccggatctc gccacgcacg cgcatctcat ctgccagcgg
     3481 gcgctgtgct acaccgcggc cgccatgttc gagtgctgtc acatctacca tgccgtcagc
     3541 ggcgacctgt ggaagcagct gcaccggctc tacgttttct ccgagaacgc gggcctggcc
     3601 accaccaccg tggacgatgc catcggacgg cggacggcga cgacgtcctg cctcgccacc
     3661 tatgtgcatg tcctgctctt gcacctcgcc cagccggatg cgctttccgt gcaacaactg
     3721 gataccgttc accgctggct ggagcgctgg gatgggctgg tgacgctttc gccggagccg
     3781 ctggcgcaga gcgctattcc cgcgctcgcg gtggatatga cctcgcaaaa aggggcgggg
     3841 ctggccaggc agatgccggc cgccggcgtg cgtcatctca atctggaggc tctcagcaag
     3901 acgttgcgcc agaccgccgc cgcgctcaag cagcagacgc cggcccagct cggtctgggc
     3961 gatctgccgc gcgaggcgtg cgaaaagctc ctgctgctgc tgcaagttca atggtgcgcg
     4021 gccggcaccg gccgcgtcga cgagcgcacg ccggcctcga tcaaggtgat gatctcgccc
     4081 aatatcgcct cgatgcactt tcacctcacc ggcaaggcgt tccgccggcc cgtgggcgag
     4141 atcaccgccg ccgagcggca acggctcgac atgttgggct ccgtatccga agccggcgag
     4201 caggcgctgg cgtcgcagcg cagcgctgcg ctcgaaacct gggtgatcct caaccagagc
     4261 gtatcgggat tcctcggtac ccgccgcaag gaggaacccg ggagtcgcat ctgttactac
     4321 cagctcgtcg ccctccagcc ggccgcaagg aaggtcatgt acgtcggcat cgtgcagcgg
     4381 ctgatcgtgg acgaggcggg aacgctgtgg ataggactgc gtatcatcct cggcgcgccg
     4441 caggccgtca ccgcgcgcat cgtagagggc gccgggaaat acgaccgcgc attgctgatg
     4501 cccgaagatg ccgcgcacaa gattcccgct tccatcgtgc tgctgcccgg ctggtatcag
     4561 gccaatcgca cgttggggct gcacaccgac aaggaaaagc agcggcgcgt caagcttcgg
     4621 gccctgctcg acaagggagt gaatttcgag cgtgcaacct acatcgccgc ctagacggcg
     4681 atgtaggttt aaaagttcaa ggttcaaggt gggaacgggt tgcgtttcga aggttctgac
     4741 ttcgaatttg gaactttgaa cccggaactc gaaacgttct tctcgcccga ttacgtttcc
     4801 gcgcgggaac gctttcgcgc cgcggcgctg gccgcggggg cggcgcttga tgcgctggcg
     4861 atcgaggcac gcgggccgcg acaggaaccg ctctccatcg atatcgcgcg cctcggcgag
     4921 cgcgatgcac ggcgcgtcct gctgcacacg tgtggcctgc acggcgtgga ggcttttgcg
     4981 ggttccgctg tgcagctcgc cgccctcgaa gacccgcccg tgccgcctgc cggctgtgcg
     5041 ctggtgctgg tgcacgcgct caacccctac ggcatggcat ggctgcggcg cgcgaacgag
     5101 aacaacgtcg atctcaaccg taattttctg cgccggggcg agcgctggga gggcgcgccc
     5161 gctctctacc cccgtctcga ccccctgctc aatccgccca gtccgccgca gccggagggg
     5221 ttccgcctaa ggctcgccct ggttgcgcta cgctacggca cgcgggcgcc ccggcaggcg
     5281 atcgcggaag gtcagtacga attcccgcgc gggctgttct acggcgggaa ggcgctcgag
     5341 ccagggccgc ggctgtatct cgactggctg cggcgcaacg tagcgcgagc cgtgtacctg
     5401 ttcgcgctcg acctgcatac cggactcggc cggcggggca aggagacgct gatactcgag
     5461 cccggcgtcg gcgcgacccc ggcaaccgag ctcacgcgcg ccttcgggag atcgctcatc
     5521 gatccggctt ccggccgggc agcgtaccgg atccgcggcg gaatgggcgg cgtgctgccg
     5581 gaaatcctgc ccggcgccgg catcgacttc gtgctgcagg aaatcggcac ctccccggcg
     5641 tccgccgtgt tgcgcgcgct gcgcgaggaa aaccgctgcc atcattacgg tgcggccgac
     5701 cttcgccacc ccgcgaagcg ggctctgctc gaggcgctgt gcccggcgct ggcggcctgg
     5761 cgcagacgcg ctgtcgagcg cggcctttcc ctgctgcgca cggcggcggc gtggacattc
     5821 cgctacgagc cgtaagcagt aagcggtaaa ccgtcagcag aaaaaccccg actcaccgct
     5881 taccgctcac cgcttacgac tgaaagggac agtatgaaat gcgaactgtg cgaaaccgac
     5941 ggcggcaccc tgctctggcg cgacctgcag tgccgcgtgg ttcacgtccc cgaggccggc
     6001 tatccgggtt tctgccgcgt gatctggaac gcccatgtag gcgagatgac cgacctttcc
     6061 gcggccgagc gcgccgactt gatgcgcgtc gtgttcaagc tggaagccgc gttgcgaaag
     6121 ttgctccgcc ccgacaagat caacctcgcg agcctgggca acgtcacgcc ccaccttcat
     6181 tggcatgtga tcccgcgttt ccgcgacgac ccgcactttc cgaacccgat ctggggcgcg
     6241 ttgatccgac agccgggtcc gctggcgcgc acgagcgctg atatcacagc agcacttgcc
     6301 cgagaaatgt cccggctata agctaaccgg ccgtcggatt ggaaacgtga tttatttccg
     6361 gaggttcgct gcgacatttg gagtgcgggt gaattaattc acataccctg ttgttgcagt
     6421 taagctagtc tgctgttgga gcggtgcgct gttcgccgcc agtcgcgacc atccgccaga
     6481 agatggcgcg gcggcggcac aggtcacgcc gggccgggcc atcgtctccg ggggaacaaa
     6541 gatccagtcc tggcgtgcgc atatacacgg aatacccatg ataagcactc tgccgcattc
     6601 gtcagctccc gctcctctgc agttcaccga cagcaccggc tgcaaacgct ggatcgagca
     6661 gcttacgctc accaacgtcc agttgaccca gcaggtcctc accggacagc ttgtgagcct
     6721 cggggcgacg ccgctgccgc cgctcgagcg cctgaagatc ctcgaaacgc tgcgggagcc
     6781 ggtgcagttc gtgcagggcg aatcggcgaa acgctatgcc ggcaagccgc tgcccctcga
     6841 tcccggcgag acgacggtat gggggaacgt catcacgttg tggcaggaaa tgagccgcaa
     6901 ctatcagcaa tgcctcaagg cctaccgtga gggcgagctc gcgatcgcac cgcatgcggc
     6961 gctcatcacc atgcgctgcc tgcgcctgct gggctgcacc ctgctcgacc actaccgtgt
     7021 ctaccggcag ccgccgggcg cgctctggcg cgccctgcac gagctccatg ccttcggcga
     7081 gagccacggc ttcgcgcgca tccgcgttca ggacagtttc tcccagcgcg atcccgattc
     7141 gagctgcgcc gagagctacg tgcaggtgct gctcgcccac ctggtcaatc cgtattcgct
     7201 gtcgctgcgg cagatggctt tcgtcgcgcg ctggctcgag cgctggtcga ccctggtcgg
     7261 gctcgccgcc cagtcgctgc cggccggccc gattccttcg ctcgccgtca atctcaccag
     7321 tacggagggc gtggtgttgg gggcagggct ggagccgcag ccgcacctgc gctacctcga
     7381 tctcgagcag ctgtcgaaaa cgctgcgtca gaccatcaac ctgctcaagc agggccagac
     7441 gccgggacag ctggggctcg gcgaggacgc gcggcaaccg ggctgcgaga acctgctgat
     7501 gctgctctac gtgcagtggt gccgcgccgg caccgcgcgc gtcgaggagc gctcgaagag
     7561 cgaggagttg gcccaggtat gcttcggtct ggcggccgcg catctgcacg tgggcggccg
     7621 cagcttccgc caaccgggcg agttgaccgc gcgcgaaaaa caggacatgg acacctacgg
     7681 ctatatcacc cggaccgagc acgaggtcag cggaaaaagc gaggcgctcg agcagtggca
     7741 gatcctcaac cagagcgctt ccggcttcat gtgcatacag cgcgagccgg agggcaaggt
     7801 gcgcgtcagc cacaaccagc tgctcgcggt gcgccgcgcg ggcggccact attttcacct
     7861 cggcatggtg cagtggctaa ggatggagga aggcggtgcg ctctactgcg gcgtgcgcct
     7921 gttccccggg acgccgcagg cgatctcggt gcgcccgtcc aactttgcgc cgaccggcag
     7981 cagccgctat gagcgcgcgc tgctgctgcc ggaagtgccg gcgcccgcca cgccggccac
     8041 cctgatcctg cccgcgggct ggttccagag cggtcgcttc ctggaaatat tctccgaccg
     8101 caagcaggtc gcgaaactgc tcaacctgct ggagaagggc ggcgacttcg accgcggaac
     8161 cattgtggtc gtgtaaacga ccacaatggt tgaatggtcc agagcgcgaa agccgacaaa
     8221 cggggccgcg ccgcccgtca tttcagaaac tcaatcgtcg ggacgcagat aaccccagtc
     8281 gcgccccagt tttctgtttc gggtcgcctc ggatatcatg aatccctctg gagggattca
     8341 tggcaggact caacaagggc gtgccgcagc cgactgaaat caggctgcac cagaaatcgc
     8401 gcatcctcga gatcgccttc gccgacggaa aggcgttcag gctgccgtgc gagttcctgc
     8461 gcgtctattc gccctcggcg gaagtgcgcg gtcatggccc cgggcaggaa gtgctgcagg
     8521 tgggcaagag ggacgtggaa atcacgcgca tcgagccggt cggcagctat gcggtgcagt
     8581 tcaccttttc cgacggccac gacaccggca tctactcgtg ggacctgctc tacgactacg
     8641 gcatgcgcca ggaggagatg tggcggcact atctcaagcg catggaggag gcgggcgcga
     8701 gccgcgaacc aaaagaggta agcagtaagc ggtaagcagt aaacggttat ttccgctcac
     8761 tgctcactgc tcaccgctca cggatgacaa agaccaccga cttcggctat cagaaggtcc
     8821 ctgaggacga acagccgcgg cgcgtcgcgg gcgtcttcga ctcggtcgcg tcgcgctatg
     8881 acctcatgaa cgacctcatg tcgggcgggc tgcaccggct gtggaagcgc tttacggtcg
     8941 atgcaagcgg gatgcgccc
//
LOCUS       DBNGWZ010000513         6101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2839325, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000513 DBNGWZ010000000
VERSION     DBNGWZ010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2839325"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="AC16CX_15705"
     CDS             <1..338
                     /locus_tag="AC16CX_15705"
                     /inference="COORDINATES: protein motif:HMM:NF020680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HIR3013988.1"
                     /translation="KAVADNGFDALRLAEQFRGQEPALIVSWLQKWSFDLVCHKLTGR
                     VRYNPDFAAAIPAVAERIDPLDAVRFLRHVVRLQRIVSHPLNARLFLEQLLLMYAALL
                     RRQPLELAA"
     gene            360..1148
                     /locus_tag="AC16CX_15710"
     CDS             360..1148
                     /locus_tag="AC16CX_15710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3013989.1"
                     /translation="MLVDSHCHLNFPELVSRVDDALALMRENGVTHALCVSVTLEEFP
                     QVRALAERYPQIYGSVGVHPDYPDVPVVSEEELVALANHPKIVAIGETGLDYYRIKGS
                     LDWQRERFRIHIRAARRCGKPLIIHMREAAADILRIMREEGAGEAGGVMHCFTGSWEE
                     AQAAIELGFHISFSGIVTFKNAEALRAVAKKVPLERMLVETDSPYLAPVPFRGKTNEP
                     GLVKYVAEEIARQRNLPLEQVALITSRNFFKLFNIKDARPHVQH"
     gene            1123..1773
                     /locus_tag="AC16CX_15715"
     CDS             1123..1773
                     /locus_tag="AC16CX_15715"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR3013990.1"
                     /translation="MQDLMFSIRKILGRGVLLIAATAATAGVYEDMLLAIDNDDGRTV
                     AELLKRGVDVNTVGPNGDSLLMLAARRGRPQMVKTVLAARPRINAQNAYGETALMLAA
                     FQGHTEVVRELLAQGAEINHAGWTPLIYAATQNRMDIARILIGRGSQINAQAENGTTA
                     LMMAAREGHLSMVLLLLEHGADINYKTRAGNTALGLARDHGRREIVEMLSRAGAVE"
     gene            1960..2145
                     /locus_tag="AC16CX_15720"
     CDS             1960..2145
                     /locus_tag="AC16CX_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013991.1"
                     /translation="MAGLRRNSEETLARYQPFWTFVAIVHGVVCIAALGGAIARIVMD
                     RRKKMPVQGDAGYKKPT"
     gene            complement(2184..3005)
                     /locus_tag="AC16CX_15725"
     CDS             complement(2184..3005)
                     /locus_tag="AC16CX_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR3013992.1"
                     /translation="MTLQEWIADSMLGRPSHWWMRHLAAGLITLLSIFIVAVLWRRLG
                     AIADPGLILLFTVAASTYLAGGMAGMLSAAIVLFCSFVLFSHPLYPFRYSDMDWRQMM
                     VVAVASPLIALMVGSLKEQVDQLKIVTKEVQALKDEIRRLEAVKEEQFLCEQRFRLMA
                     DSLADYAVCMLDANGLVRNWNSGAEHVLGYTDPEILGQSYSRFFTKEDLLAKQPEQLL
                     DHARFSGRAEEEGWRLRKGGGRFRARITLLLLRNSNGMPDGCLMVMRDLTQSIEG"
     gene            complement(3238..4758)
                     /locus_tag="AC16CX_15730"
     CDS             complement(3238..4758)
                     /locus_tag="AC16CX_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3013993.1"
                     /translation="MLTSKELLEKTGISRATLNNYIARGFLPKPVVAQPEVRGGNRPR
                     QIGYFPDETLACIEHIQRLKSEGLSMDEIASRLVASAPPEEPESVPATPGKAQVISHP
                     AAAAKKQPLVLTMDQMPHPAYMVNYKSEVIWFNDLARETMGGAFSQLAPRTEERSVFR
                     LLLLAEDKATQANRDALLRLNIALAKERMEFSAITQPLQPIISAKVHLLERLYAETTP
                     LGAAPIVEYPLRLKETKGNRIENYCAYAAYFREGILVIMVPLKDDADALLQLLSRRDM
                     VIRNLLSKRLPTLTDLAVLVADLQESVKICSELPPEEYFELINQIWSSAGTVFRKYYG
                     THGKHVGDGMVYYFFPQPDCNYIMNAVVCAHELKAATAKISKEWQLRKKWANELYLNT
                     GLNEGQEWLGTFQTATTVEFAVLGETINHTARLSDFARYGAIWATKNFVGKIPAGDRS
                     RIKFGIHRRGPEGQPIFVESTYSRVGNLVDLGAGRYEKLKDIAGLSVAEIVNVVSE"
     gene            complement(4881..6083)
                     /locus_tag="AC16CX_15735"
     CDS             complement(4881..6083)
                     /locus_tag="AC16CX_15735"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR3013994.1"
                     /translation="MLDAYLYEGVRTPFGRHAGVLAKVRPDDLLADVIKNLMKKSAFK
                     PEQIEDIVVGCANQSGEDSRCVARHAGLVAGLPIETPGTVLQRNCASGLGAVVHAAHA
                     ITAGEGDVFLAGGVESMSRSPLVMSRSESAFGRDVRLYDSTIGPRFSNPKLVKRFGDD
                     QMPQTADTLAREYGIKREEADKFALRSQQKYSIAKGEGFFSGEIVPVELPPSRKGPVP
                     PVTDDEHPRPNTDLETLSRMKTLYEGGVTTAGNASGVNDGAVALIVASREAGEKAGVK
                     PLARVIASAAAGVPPQIMGIGPVAASQKALARAGLSLKALDVIEINEAFAAQVLACLK
                     GLGVAFDDTRVNPNGGAIAVGHPLGASGGRLALTAARQLQRSGGRYALVSLCIGGGQG
                     MAAILERV"
BASE COUNT         1174 a         1858 c         1885 g         1184 t
ORIGIN      
        1 tcaaggcggt cgccgataac gggttcgatg ccctgcgtct cgccgagcaa ttccgtggcc
       61 aggagccagc gctgatcgtg agctggctgc agaaatggtc gttcgatctg gtttgccaca
      121 agctgacggg gagggtccgc tacaatccgg acttcgccgc cgccatcccc gccgttgccg
      181 agcggatcga tccgctggac gccgtgcgtt tcctgcgcca tgtcgtgagg ctgcagcgca
      241 tcgtttccca tccgctcaat gcgcggctgt tcttggagca attgctgctg atgtatgccg
      301 cactgctgcg acgccagccg ctcgagttgg cggcgtgatt gcaggcgccg gacgggctca
      361 tgctggttga ttcgcattgc cacctcaatt ttccggagct cgtcagcagg gtggacgatg
      421 cgctggcatt gatgcgggaa aacggggtca cgcatgcgct ttgcgtcagc gtcacccttg
      481 aggaatttcc gcaggtgagg gcgctcgccg agcgctatcc gcagatctac ggctcggtcg
      541 gcgtgcatcc cgattacccg gatgtccccg tggtcagtga agaggagctg gtcgcgctcg
      601 cgaaccaccc aaaaatcgtg gcgatcggcg agactggcct cgattactac cggatcaagg
      661 gcagtctcga ttggcagcgc gagcgctttc gcatccacat ccgtgccgcg cgccgctgcg
      721 gcaagccgct gatcatccac atgcgggaag ccgcggccga catcctgcgc atcatgaggg
      781 aggaaggagc aggagaggcc ggaggcgtca tgcactgctt taccggcagc tgggaggaag
      841 cgcaggctgc gatcgaactc ggtttccaca tctctttttc ggggatcgtc accttcaaaa
      901 acgcagaggc gttaagggcg gtggcgaaga aggtgccgct ggagcgcatg ctggtggaaa
      961 ccgattcacc ctaccttgct cccgtgccgt tccgggggaa aaccaatgag ccggggctgg
     1021 tcaaatacgt ggccgaggaa atcgcccgac agcgcaatct cccgctcgag caggttgcgt
     1081 taatcaccag cagaaatttt ttcaaactat tcaatatcaa agatgcaaga cctcatgttc
     1141 agcattagaa aaatcctggg caggggagtc ttgctgattg ccgccaccgc agctacagcc
     1201 ggggtttatg aagacatgct gcttgccatc gataacgacg atgggcggac ggtagccgaa
     1261 ctcctgaagc gcggggttga cgtcaacacc gtcggaccga acggagactc gctgctgatg
     1321 cttgcggcga ggcgcggcag gccgcaaatg gtgaaaaccg tgctggccgc ccggcccagg
     1381 atcaacgcgc aaaacgctta cggggaaacc gctctcatgc tggcggcttt ccaaggccac
     1441 accgaagtag ttcgcgaact gctggcccaa ggcgccgaga tcaaccatgc ggggtggacg
     1501 ccgctcattt acgcggcaac ccagaaccgg atggacatcg ctcgcatcct gatcggacgg
     1561 ggctcccaaa tcaatgccca ggcggaaaac ggaaccacgg cgctcatgat ggcggctcgt
     1621 gagggacacc tgtcgatggt cctgctgctg ctggagcatg gtgcggatat caactacaaa
     1681 acccgtgcgg gcaatacggc gctgggcctg gccagggacc acggacggcg cgaaatcgtt
     1741 gaaatgctga gccgggccgg ggctgttgaa taggccccag aactccttgg ataagccgcc
     1801 cttgcagaca gtctttcttt gcatagatat tcgtataatg gttattatgt aaaatatcaa
     1861 catgtgatgt atagggcgac agaactcttg ggataccctg tccacctccc ccggactcag
     1921 tgtgaaagcg atcagagcga ttgaagccag aatagtcagg tggctggcct acgccgaaat
     1981 tccgaggaaa cgctcgccag ataccagccg ttctggacct ttgtcgcgat cgttcatggc
     2041 gtcgtctgta tcgcggcgct gggcggcgcg atcgccagaa tcgttatgga tcggcgtaag
     2101 aagatgccgg tgcagggaga cgcaggttac aagaagccaa cctgacggga ctcgcgacgc
     2161 caggccttat gagaggaggt gccctaccct tcgatggact gggtcagatc gcgcatgacc
     2221 atcaggcagc cgtcgggcat gccgttcgag tttctcagca gtagcagagt aatcctggcg
     2281 cggaagcgcc cgccgccttt gcgcaggcgc cagccctcct cttccgcccg tcccgaaaag
     2341 cgcgcgtggt cgagcagctg ttccggctgc ttggcgagca ggtcctcctt ggtgaagaac
     2401 cgcgagtagc tttgtcccag tatctccgga tcggtatagc cgagcacgtg ttctgcgccc
     2461 gagttccagt tcctgaccaa tccattggca tccagcatgc acacggcgta gtccgccagg
     2521 ctgtcggcca tgaggcggaa tcgctgctcg cacaggaatt gctcttcctt gacggcttcg
     2581 agccggcgga tctcgtcctt cagggcctgg acttctttcg tgacgatctt cagctggtcc
     2641 acctgctcct tgagcgagcc caccatcaga gcgatcagcg ggctggcgac cgcgaccacc
     2701 atcatttgac gccaatccat gtcggaatag cggaacggat acaaggggtg cgagaacagc
     2761 acgaaggagc agaacaggac aatggccgcg ctcagcatgc cagccatgcc ccccgcgaga
     2821 taggtggaag ccgccaccgt gaacagcagg atcagtcccg ggtcggcgat tgcgcccagg
     2881 cgacgccaca acacggcgac gatgaatatc gacagcagcg tgatgaggcc cgccgccaga
     2941 tggcgcatcc accaatggga tgggcgtccc agcattgagt cggcgatcca ttcctgcaac
     3001 gtcatggcta ttccaagctt atgcaagacg acggtttttc gtcattgacc gtataaattc
     3061 agccggttaa gcggctaaaa gcccattccg gcggctatcc ggacagatcg cgcaagaaca
     3121 atgccaatgc acagcctgaa cggctatgaa attccacacg gttcaatcag ttacggggaa
     3181 aacgcgactc gatctgacgc gatgagaacg gcccgtaggc cggccacaca agaggcgcta
     3241 ttcggagacg acgttgacga tctcagcgac ggacagaccg gcgatgtcct tcaacttctc
     3301 gtagcggcct gcgccgagat ccacgaggtt gccaacgcgg gaataagtgg actcgacgaa
     3361 tattggctgt ccttcaggac cccgccggtg gatcccgaac ttgatgcggc tgcgatcccc
     3421 cgccggtatc ttgccgacga aattcttggt ggcccagatc gccccatagc gcgcgaagtc
     3481 tgaaagacgc gcggtgtgat tgatagtctc tcccaggacg gcgaattcga ccgtggtcgc
     3541 ggtctggaac gttcccagcc attcctggcc ctcgttgagg ccggtgttga ggtagagctc
     3601 gttcgcccat ttcttgcgca gctgccactc cttgctgatc ttcgccgtcg ccgctttcag
     3661 ctcgtgtgcg cacaccaccg cgttcatgat gtagttgcag tcgggctgcg ggaagaagta
     3721 atagaccatc ccgtcgccca cgtgcttgcc gtgggtgccg tagtacttgc ggaatacggt
     3781 accggcgctc gaccagatct ggttgatcag ctcgaagtat tcctcgggtg gcagctcgga
     3841 gcagatcttc accgattcct gcaggtcggc caccagcacg gcgagatcgg tgagcgtcgg
     3901 cagccgcttg ctgaggagat tgcggatcac catatcgcgc cggctgagca gttgcagcag
     3961 cgcgtccgcg tcgtccttga gcggcaccat gatcaccaga atgccttccc ggaaatacgc
     4021 cgcgtaggca cagtagttct cgatccggtt gcccttcgtc tccttcaggc gcagcgggta
     4081 ctcgacgatt ggcgcggcgc cgagcggcgt cgtttccgca tacaaccgct cgagcaaatg
     4141 caccttggcc gatatgatcg gctgcaacgg ctgggtgatc gccgagaatt ccatgcgctc
     4201 cttcgccagc gcgatgttca ggcgcagcag cgcatcgcga ttcgcctggg tcgctttgtc
     4261 ttcggccagc agcagcagcc ggaaaacgct gcgctcctcg gtccgcggcg cgagctgcga
     4321 aaatgcccca cccatcgttt cccgcgcaag atcgttgaac cagatcacct cggacttgta
     4381 attgaccata taggccgggt gcggcatctg gtccatggtg agcaccagtg gctgcttttt
     4441 tgcagcagcc gcggggtggg aaatgacctg agccttgccg ggggtagctg gcacgctctc
     4501 cggttcttca gggggggcgg atgcgaccag cctcgaagcg atttcatcca tgctcaatcc
     4561 ctcgcttttc aaccgctgga tgtgctcgat gcacgccagc gtctcgtccg gaaaatagcc
     4621 gatctgccgc ggccggttgc caccccgcac ctcgggctgt gcaaccactg gcttgggcaa
     4681 gaaaccccgg gcaatgtaat tattcagagt agccctggag atgcctgttt tttcaagcaa
     4741 ttccttgctg gtcagcatat tgtataatca gtccacatag actgtttata catatgctga
     4801 tacaagtcca ttcattagtc aagctgacta ttcaggagaa ctctgcagtg ggcccgacca
     4861 gtggttctgg cggtacggaa ttagacgcgc tcgaggattg ccgccatgcc ctgaccgccg
     4921 ccaatgcaga ggctcaccaa agcatagcga cctcctgagc gctgcagctg ccgtgcagcc
     4981 gtgagggcaa gccgccctcc tgaggcgccg agtggatgcc cgactgcgat ggcgccgcca
     5041 ttgggattga cgcgggtgtc gtcgaaggcg acgccgagcc ccttcaggca ggccagcacc
     5101 tgggcggcga aggcctcgtt gatctcgatg acgtccaggg ccttgagcga taggccggcg
     5161 cgcgccagcg ctttctggga cgccgcgacc ggcccgatgc ccatgatctg ggggggcacg
     5221 ccggccgcgg cgctggcgat cacgcgcgcg agcggcttga cgcccgcttt ctcgccggct
     5281 tcccgggaag cgacgatgag cgcgaccgcg ccgtcgttga cgcccgatgc attgcccgcg
     5341 gtagtaaccc cgccctcgta gagggtcttc atgcgcgaga gcgtctcgag gtcggtgttc
     5401 gggcgcgggt gctcgtcatc cgttaccggt ggtaccggtc ccttgcgcga tggcggcagt
     5461 tccaccggga cgatctcgcc ggagaagaaa ccctccccct tggcgatcga gtacttttgc
     5521 tgcgagcgca gcgcgaactt gtcggcttcc tcgcgtttga tgccgtactc gcgcgcgagg
     5581 gtgtcggcgg tctgcggcat ctggtcgtct ccgaagcgct tcaccagctt ggggttcgag
     5641 aagcgcggcc cgatggtgct gtcgtagagc ctgacgtcgc ggccgaacgc cgattccgac
     5701 cggctcatca cgagcggcga gcggctcatg ctttccacgc caccggccag gaacacgtcg
     5761 ccttcaccgg cggtgatggc gtgggccgca tgcaccacgg cgcccaagcc gctcgcgcag
     5821 ttgcgctgga gcacggttcc cggcgtctcg atcggcaggc ccgccacgag acccgcgtga
     5881 cgcgccacgc agcggctgtc ttcaccggac tggttggcgc aacctacgac gatgtcctcg
     5941 atctgctccg gtttgaaggc cgatttcttc atcagattct tgatgacgtc cgccagaagg
     6001 tcatccggcc gtactttggc gagcacaccg gcatggcggc cgaacggggt gcgcacgcct
     6061 tcgtagagat aggcatcgag catggcaatc tcctttcagg a
//
LOCUS       DBNGWZ010000514         2638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2210663, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000514 DBNGWZ010000000
VERSION     DBNGWZ010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2210663"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..137
                     /locus_tag="AC16CX_15740"
     CDS             54..137
                     /locus_tag="AC16CX_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3013995.1"
                     /translation="MMKLHRNLKRTLDPAGILNPGRMYPEY"
     gene            247..744
                     /locus_tag="AC16CX_15745"
     CDS             247..744
                     /locus_tag="AC16CX_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3013996.1"
                     /translation="MANNFLEQLVTEWYEYRGYFVRRNVWVGKQGRGRFESELDIVAF
                     DPRTRHLVHVEPSMDAESWEKRERRFRKKFDAGRKHIPGLFPGLDLPEEIEQIAILAF
                     ASKVNHPTLGGGKVLLVSDLMREIIENLSDKKIAADAVPEHHALLRTLQFVIEYRKKV
                     FAALR"
     gene            757..1983
                     /gene="glcF"
                     /locus_tag="AC16CX_15750"
     CDS             757..1983
                     /gene="glcF"
                     /locus_tag="AC16CX_15750"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR3013997.1"
                     /translation="MQTSLADFIRDTPEGREADAILRKCTHCGMCTATCPTYQLLSDE
                     LDGPRGRIYLIKQVLEGATPTARTQLHLDRCLTCRACETTCPTGVRYGRLLDIGRNLV
                     EERVARGPLETAWRGALNAVIPNSSLFGPLLKLGQVTRSVLPSALKRKVPPAAAPAGP
                     WPVTVHARKMLVLDGCVQPSLSPATNAATARMLDRLGITLVRAQGAGCCGGVSFHLNR
                     QESALDTMRATINAWWPHIQQGTEAVVITASGCGVTVKDYGHLLSHDPAYAEKAKRVS
                     ELAKDISEIIIAESTKLNLLVTRQRIAFHSPCTLQHGLKMKGAVEKLLVDLGYSLTPV
                     PDAHLCCGSAGTYSILQPELSRQLLANKIAALSSGAPAAILTANIGCQAHLQTATDLP
                     VRHWIEALDDSLPNRD"
     gene            2177..>2638
                     /locus_tag="AC16CX_15755"
     CDS             2177..>2638
                     /locus_tag="AC16CX_15755"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR3013998.1"
                     /translation="MLGLNPHTASRNLEVLSRSEMPDKEVADSTTSLSVPVYQQEFWT
                     SRQRQGSSIHEISYRACFKPQLPAYFIERLTKEGDVVYDPFSGRGTTAVEAALQGRTV
                     VANDVNPLATIFIRPRLELPGIADIDKRLRSLRFAANAATDINLSMFYHPNT"
BASE COUNT          525 a          858 c          789 g          466 t
ORIGIN      
        1 tgttccgcgg cggcgacaag tcggcgggcg tatttcaccc cctctcgcca gctctgatga
       61 agctgcaccg caacctgaaa cgcaccctgg acccggccgg catcctgaac cccggcagga
      121 tgtatcctga atactgatga gcggtgagca gtgagcggtg aaccgtaagc ggtaaactcc
      181 ttagagaatt ttcccacccg cttttaccgc ttactgctta ccacttaccg ttcacggaat
      241 ttaacaatgg cgaacaactt cctcgagcag ctggtcacgg agtggtacga gtaccgcggc
      301 tacttcgtgc ggcgcaacgt ctgggtgggc aagcaggggc ggggcaggtt cgagtcggag
      361 ctcgatatcg tcgccttcga cccgagaacg cgccatctcg tgcacgtcga gccttcgatg
      421 gatgccgaaa gctgggagaa gcgcgagcgg cgcttccgga agaaattcga cgcgggacgc
      481 aagcatattc ccggcctgtt cccgggcctc gatctgccgg aggagatcga gcagatcgcg
      541 atcctggcgt tcgcgagcaa ggtgaaccat ccgaccctgg gcggcggcaa ggtgttgctg
      601 gtgtccgatc tgatgcgcga gatcatcgaa aacctgagcg acaagaagat cgccgcggac
      661 gccgtgcccg agcatcacgc gctcctgcgc acgttgcagt tcgtcatcga ataccgcaag
      721 aaggtattcg cggcgctgag gtagcgaggc gcgcccgtgc agacaagcct cgccgacttc
      781 atccgcgaca cgcccgaagg acgggaggcc gacgcgatcc tgcgcaagtg cacgcattgc
      841 ggcatgtgca ctgcgacctg cccgacgtac cagctgctca gtgacgagct cgacggcccg
      901 cgcggccgga tctacctcat caagcaggtg ctcgagggcg caacgccgac ggccaggacg
      961 cagctccacc tcgaccgctg cctgacgtgc cgtgcgtgcg aaaccacgtg cccgacgggc
     1021 gtgcgctacg ggcgcctcct cgacatcggc cggaatctcg tggaagagcg tgtcgcccgg
     1081 ggtccccttg agacggcctg gcgcggggcg ctgaacgcgg taatcccgaa ttcctccctg
     1141 ttcgggccgt tgctcaagct gggacaggtg acgcgctccg tccttccgtc cgctctcaaa
     1201 cgcaaggtgc cgcccgcggc ggcaccggcc gggccgtggc ccgttacggt tcacgcgcgc
     1261 aagatgttgg tactcgatgg ctgtgtccag ccgagcctgt cgcccgcgac caatgccgcc
     1321 acggcgcgca tgctcgaccg gctcggcatt actcttgtga gggctcaggg cgccggctgc
     1381 tgcggaggtg tgtcctttca tctgaaccgg caggagtcag cgctcgacac catgcgcgcg
     1441 acgatcaacg cctggtggcc acacatccag caaggtaccg aagcggtagt catcacggcc
     1501 agtgggtgcg gtgtgacggt gaaggattac ggccacctgt tgagtcacga tccggcctac
     1561 gcggagaaag ccaagcgcgt ctcggaactg gcaaaagaca tcagcgaaat catcatcgcc
     1621 gaatcaacga aactgaacct actcgtcact cgtcagagaa tagccttcca ttccccctgc
     1681 acgttacaac atggattgaa gatgaaggga gcagtggaaa aactgctagt cgatctcggc
     1741 tactcactca cccccgttcc cgatgcgcac ctctgctgcg gctcggccgg tacctattcc
     1801 attctgcagc cggagctttc ccgacagctc ctcgccaaca agatcgcggc cctttcgagc
     1861 ggcgcgcccg ccgccatcct caccgccaat atcggctgcc aggcgcacct gcagacggcc
     1921 accgaccttc ccgtccggca ctggatcgag gcgctggacg acagcctgcc gaatcgggat
     1981 tgatcaaacc cgagcgcgag tcttcgccca gtcaggaaca agcgcaccag cggcaggctg
     2041 tagcgcggca atacgagtcg aacgcccgta tgaccagacc tagtacctca ccttaagtac
     2101 gagctgtaac ccctctgtac atttgagaaa aattggagta cgatgctcct ccgggtaatt
     2161 cgatggagga accatcatgc tcgggctcaa tccgcatact gcatcgcgga atcttgaagt
     2221 attaagccgt tctgaaatgc cggataagga agtcgctgac tcaacgactt ccttgtcggt
     2281 gccggtctac cagcaggagt tctggaccag ccgccagcgt caggggagct cgatccatga
     2341 gatctcctac cgcgcctgct tcaagccgca gctgcccgcc tatttcatcg agcggctgac
     2401 gaaggaaggc gacgttgtct acgacccgtt cagcggccgc ggcaccaccg ctgtggaggc
     2461 cgcgctgcag gggcgcaccg tcgtcgcgaa cgacgtaaac ccgctggcga ccatcttcat
     2521 ccggccccgg ctggaactgc ccggcattgc ggacatcgac aagcggttga ggtcgctgcg
     2581 gtttgcggcc aacgccgcca ccgatatcaa tctgtcgatg ttctaccatc ccaacacg
//
LOCUS       DBNGWZ010000515         6434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_823180, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000515 DBNGWZ010000000
VERSION     DBNGWZ010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_823180"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1242
                     /locus_tag="AC16CX_15760"
     CDS             76..1242
                     /locus_tag="AC16CX_15760"
                     /inference="COORDINATES: protein motif:HMM:NF025934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047487 - oligogalacturonide lyase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate lyase family protein"
                     /protein_id="HIR3013999.1"
                     /translation="MTVGTVFAPEWRTERDSQTGRTVTQLTDSPAEDYHPYFYNPAVT
                     RDGRYLLFFSERTGLSNLFRLDFRSGEIVQLTDARPARADYWPFTAVVRGAGACLAAI
                     GSGGREAFYFEGVELWGVEIETMRARRLLALPDDRRPSILHADAEGRTLVFATWDEAL
                     FRERSQRAYAGEQFPDDQFFQETASTIMRVDTATGCAEEILRRERFWINHVLVNPYDR
                     DLVLFCHEYSALRDRMWLLNAATRQCAPIPGQGDDEWYEHEFWSLDGRRVCFHGGPLN
                     DETRGFCGWCSPDGAEYHKFEHVTPGRAYAHYNLHPDGTTMVTDGEAQPGCVSKVLLK
                     DGRQDFEVLCRHDSYRFGDDQRCHPHPSFAPDGSRVIFTSNRTGSSNVYVTDWK"
     gene            1253..2236
                     /locus_tag="AC16CX_15765"
     CDS             1253..2236
                     /locus_tag="AC16CX_15765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3014000.1"
                     /translation="MKFGFITTEGGAFYREALEEAVYGEELGFDSVWLEEHHSIRNHY
                     WPSPLMALAGFAARTSRILLGTDIVVLPFYHPVRAAEDAAMLDIMSDGRAIFGAAIGY
                     RPPEFDLFGVSLDDRGARYVEMLKIMRALWTEDQVDYAGRFFQVKGAIEPKPARPIPL
                     WLGGWGELSLKRAAELGDAWVPGPTANLQKLLDAQAQYRANLKAVGKDPSATPTPLTR
                     EVVIAARREQAWELAEKYVMVNYRDEYGGGWKHPLIGAQDSTPVNQLEALGQDRFIVG
                     DPAECRQKIRRFVEAFGVDHLICRLYFPGMPHEHIMHELKLLATEVFPAFR"
     gene            2236..4542
                     /locus_tag="AC16CX_15770"
     CDS             2236..4542
                     /locus_tag="AC16CX_15770"
                     /inference="COORDINATES: protein motif:HMM:NF021534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIR3014001.1"
                     /translation="MTEWLPPLNYDLSPYTGWTREHWEAVLARLTYGYVLAAERQGSP
                     ARALFPDDRCDRPDAVDALEAFARLGAGWGAWLRNPANPACLTFQGREINLEALLSQA
                     LLDGLDPTNPRTYWGDIGHMDQRLVEAAGVAVTVWLSRERVFNRLTGAERARVMDWLA
                     QVDGKETWPDNWILFPAMAQVTRLNLGYPIPESDLDSRLGQMAAFYRGDGWYADGPGD
                     EFDLYNAWMFGWHYLLWAWMDGDRRPPHRDLVLKRARAFLDGFQYFFGANGSHVAWGR
                     SLLGRFATTVAFQIGHWLKIAPPRPGLLRRICSGCLRYFVEHGFFDPEGHFARQGFHE
                     AFPLAGESYVAPGSPLSACHALFALTFDRGDPFWTEPESPLPVESEDFDLAFPVPGLA
                     VSGRRATGQVILLNGRSGHPADVPRPDYTPKYGKFAYTTHFPFNVAPVAVSYAPDAMI
                     ALTHDGLTFGHRDTTLAGGAGPGVIWCEYDESVAGQPQRVCAAVLIWRDLQLRLAAVR
                     PSLAVRAFEAPGALGCEGAAKVMRSSEPAAGWEYAEAEGRALGIRRLLGYDGQQPSAP
                     FLGYSNLNLAYAYSEQPLVCETQASALPRALAAVSLLRPAPFDPVREFAGVSVTALSI
                     DSFRITLPDGEEAFVALGDGPPLSASLGQLSFEGDALRCVRVSRDASQICGLGVREVA
                     GIASLSAPGTLRLRREAGQVAHVTTDVGVSLTAEWLGGSAQRIEALALGGEWTDVTED
                     CRDNVIPPDVVRRWSQQNERSLVKFRLS"
     gene            4542..>6434
                     /locus_tag="AC16CX_15775"
     CDS             4542..>6434
                     /locus_tag="AC16CX_15775"
                     /inference="COORDINATES: protein motif:HMM:NF021534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HIR3014002.1"
                     /translation="MMEVFPALNYDLSPYTGWTREHWEAVLARLTYGYVLAAERCGSP
                     ARALYPDDRRGLPDSVDALESFARMASAWGAWLRNPANPAGLTFQGREIDLEVLLRQA
                     LLDGTNPQNPHTYWGDMDHMSQHIVEAADVAVTVWLSRERVFDRMTEAERAQVMNWLA
                     QVDGKQTYFDNWILFPAMAQMVRMKLGYPAPEADLDARLEQMAAFYRGDGWYADGPGD
                     EFDLYNAWLFGWHYLLWAWIDGARRPDHRRLVLDRARAFLNGFQYFFGANGSYPAWGR
                     SIVYRFSAVAAFQTGHLLNVAPSDPGLLRRLSSGCIRYFYERGLLDPGAHHIRQGFHG
                     DFPPAGESYISPGSPYWACHGLFALTFDRDDPFWTATEAPLAVEREDFDLALPAPGFV
                     LSGRWATGQVILLNSRSSQAEEIVPDAGGGQLTSEAELMTRFAPRHDYPAKYGKLAYS
                     THFPFNVAPVPGSYAPDAMVALSQDGQRFGHRSTTRAGGVAPGVMWCEFDEMVDGQPQ
                     TIHLAVLLWKDLQVRVALLQPTLPVRAFEAPGALGCDCASKIVRRSDPDAGWEYAEAD
                     GRALGVRRLLGYDGQQASAPFLGYSNINLAYLYSEQPMVYETRPSAALRGLAAVSLLR
                     PAPFDPA"
BASE COUNT         1025 a         2028 c         2131 g         1250 t
ORIGIN      
        1 cgccgaggtc gcactcccac acgccctcaa agccatgcga ggcctgctgg cggctcgtgc
       61 cctggagaaa tcgccatgac cgtcggcacg gtctttgccc ccgagtggcg cacggagcga
      121 gactcgcaga cgggccgcac agtcacgcaa ctgacggact cgcccgcgga agactatcac
      181 ccctactttt acaacccggc cgtcacgcgg gatggccggt atctgctctt tttttctgag
      241 cgcaccggat tgagcaatct ctttcggctc gactttcgct ccggagaaat tgtccaactc
      301 acggatgcgc gtccggctcg cgccgactac tggccgttca ccgcagttgt gcgcggggcc
      361 ggggcgtgcc tggccgcaat cggctccggc gggcgagagg cgttctactt tgagggcgta
      421 gagctgtggg gcgtcgagat cgagactatg cgagcgcggc ggttgttggc tctgccggat
      481 gaccggcggc cctcaatcct gcacgccgat gcggagggca gaaccctggt attcgcgacg
      541 tgggatgaag cgctcttcag ggaacgctcg caaagggctt acgccggaga acaattcccg
      601 gacgaccagt tctttcaaga gacggccagc acgatcatgc gcgttgacac ggcgacggga
      661 tgcgccgaag aaatcctgcg ccgggagaga ttctggatca atcacgttct ggtcaatccg
      721 tacgaccgcg atctcgtatt gttctgccac gaatatagcg cattgcggga ccggatgtgg
      781 ctgctcaacg cggcgacccg tcaatgtgcg cccattcccg ggcagggcga cgatgagtgg
      841 tacgagcatg aattctggag tctggacgga cggcgagtct gttttcacgg cggccctttg
      901 aacgatgaaa cgcggggctt ctgtggttgg tgctcgcccg acggcgcgga gtatcacaag
      961 ttcgagcacg tcacccccgg ccgggcctat gctcactaca acctgcaccc ggacgggacg
     1021 acgatggtca cggatggcga ggcgcagccg ggttgcgtct ccaaagtgct cctgaaagat
     1081 gggcggcagg attttgaagt cctgtgccgc cacgactcgt atcgtttcgg cgacgaccag
     1141 cgctgccacc cgcacccgtc gtttgcgccg gatggaagcc gggtgatctt tacttcaaac
     1201 agaaccggat ccagcaacgt gtatgtgacc gattggaagt gagaaacgaa acatgaaatt
     1261 cggattcatc accaccgagg gcggggcgtt ctatcgcgag gcgctggagg aggcggtcta
     1321 cggcgaagaa ctgggcttcg actcggtctg gctggaggaa catcacagca ttcggaatca
     1381 ctactggccg tcgccgctca tggcgctggc cggtttcgcg gcgcgcactt cgcgcatcct
     1441 gctgggcacc gacatcgtcg tcctgccctt ctaccacccg gtgcgggccg ccgaggacgc
     1501 ggccatgctc gatatcatgt cggatgggcg cgcgatcttc ggggccgcca ttggctaccg
     1561 ccctcccgag tttgacctgt tcggagtctc gctcgacgat cgcggtgcgc gttatgtgga
     1621 aatgctgaag atcatgcgtg cgctgtggac tgaagatcag gtggattatg ccgggcgctt
     1681 ctttcaggtc aaaggcgcga ttgagccgaa accggcgcgg cccattcccc tctggcttgg
     1741 cggttggggc gaactctcgc tgaagcgtgc tgccgagttg ggcgatgcct gggtgcccgg
     1801 cccgacggcc aacctgcaaa aactgctcga cgcgcaggcg cagtatcgcg ccaatttgaa
     1861 agccgttggc aaggatccgt ccgctacgcc cacaccgctg acccgcgaag tcgtcatcgc
     1921 agcgcggcgc gaacaggcgt gggaactggc cgagaagtat gtcatggtga actaccgcga
     1981 tgaatacggg ggtggttgga agcatccgct gattggcgca caggacagca cgccggtcaa
     2041 tcaactcgaa gcgctcgggc aggatcgctt catcgtcggt gaccccgccg agtgtcgcca
     2101 gaagatcagg cgctttgtcg aggccttcgg agtggatcac ttgatctgcc gcctgtactt
     2161 ccccggtatg ccgcacgagc acatcatgca cgaactcaaa cttctggcca ccgaggtgtt
     2221 tccggcgttc cgctgatgac cgagtggctg cctccgctca actacgacct ctcgccctac
     2281 accggctgga cgcgcgagca ctgggaagcc gtgctcgccc ggctgacgta tggctacgtc
     2341 ctcgccgccg agcgccaggg ttccccggcg cgtgcgctgt tccccgacga ccgctgtgat
     2401 cgccccgatg cggtggatgc gctggaggcc ttcgcccgcc tcggcgcagg ttggggggcg
     2461 tggctgcgga acccggcgaa cccggcttgc ctcacgtttc agggtcgcga gatcaacctc
     2521 gaagcgttgt tgagccaggc gctgcttgac ggcctcgatc ccacgaatcc ccgcacttat
     2581 tggggcgata tcggccacat ggatcagcgc ctcgtcgagg ccgcgggcgt ggccgtgacg
     2641 gtgtggttga gccgcgagcg tgtgttcaac cggctgaccg gggctgagcg cgcgcgggtg
     2701 atggactggc tggcgcaagt ggatggaaaa gagacctggc ccgataactg gattctgttc
     2761 ccggcgatgg cgcaggtgac gcggttgaat ctaggctacc cgatcccgga atccgatctt
     2821 gactctcggc ttgggcaaat ggccgccttc tatcgcggcg acggctggta cgccgacggc
     2881 ccgggcgacg aattcgatct ctacaacgcc tggatgttcg gctggcatta cctgctgtgg
     2941 gcgtggatgg acggcgaccg ccgcccgcct catcgcgacc tggtgctgaa gcgcgcccgc
     3001 gcgtttctcg atggatttca gtacttcttt ggtgcgaacg gctcgcacgt ggcctggggg
     3061 cggtcgctgc tcggccgctt cgcgaccacc gtggccttcc agatcggcca ctggcttaag
     3121 atcgcgcccc cccgtccggg tctgctccgc cggatttgca gcggctgtct gcgatatttt
     3181 gtggagcatg gctttttcga tccggaaggc cacttcgcgc ggcagggatt ccacgaggcc
     3241 ttcccgctgg cgggcgagtc ctacgtcgcc ccagggtcgc cgctctctgc ctgccacgcg
     3301 ctgttcgccc tgacgttcga ccgcggcgat cccttctgga cggaacccga atcgccgctg
     3361 cctgtcgaaa gcgaggattt cgatctggca ttcccggtcc ccggcctggc ggtctctggc
     3421 cgtcgcgcta ccgggcaggt gatcttgctc aacggccgga gtggacatcc ggcagacgtg
     3481 ccacgccccg attacacgcc gaagtacggc aagttcgcgt acaccacgca cttccctttc
     3541 aacgtcgcgc cggtcgccgt gtcgtatgca ccagacgcga tgatcgcgct gacgcacgac
     3601 ggcctcacgt tcgggcaccg cgacacgacg ctcgcgggcg gcgccgggcc gggcgtcatc
     3661 tggtgcgagt acgacgagag cgtggccggc cagcctcaac gcgtctgtgc tgccgttctc
     3721 atttggcggg atttgcagtt gcgccttgcg gcggtccggc cttcgcttgc ggtgcgagcc
     3781 ttcgaggcgc caggagcgct gggatgtgaa ggcgcggcca aagtcatgcg ctcctcggag
     3841 ccggccgccg ggtgggagta cgccgaagcc gaaggccgcg cgctgggcat tcgccggttg
     3901 ctgggctacg acggccaaca acctagcgcg cctttcctcg gctactccaa tctcaatctg
     3961 gcctacgcct attctgagca gccgctagtg tgcgaaacac aggcgagcgc actgccgcgc
     4021 gctctggccg ccgtgagcct gctgcggccg gcgcccttcg atccggtacg cgaatttgcc
     4081 ggtgtgtcgg tgaccgcgct ctccatagat tcctttcgga tcactctccc ggatggcgaa
     4141 gaggcgtttg tcgcgctcgg cgacgggccg ccgctgtcgg cgtcgctggg ccaactgtct
     4201 ttcgagggcg atgccctgcg ctgtgtgcgc gtcagccggg acgcgagtca gatatgtgga
     4261 ttgggagtga gagaggtggc cggcatcgcc tcgctctctg cgccgggcac gctccggttg
     4321 aggcgtgagg cgggccaggt ggcgcatgtg acgaccgacg tcggtgtctc tctcactgcc
     4381 gagtggctgg gcggctcggc ccaacggatt gaggcgcttg cgctcggcgg cgaatggacg
     4441 gacgtgacgg aggactgccg ggacaacgtc atcccgcccg atgtcgtgcg gcgctggtca
     4501 cagcagaatg aacggtcgct ggttaagttt cgtttgagct gatgatggaa gtgtttcctg
     4561 cgctcaacta cgatctctca ccctacactg gctggacgcg cgagcactgg gaagcggtgc
     4621 tcgcccggct gacatatggg tacgtcctcg ccgccgaacg gtgcggatcg cccgcccgcg
     4681 cgctgtatcc cgacgaccgg cgcggcctgc cggactcggt ggacgcgctc gaatcattcg
     4741 cgcggatggc gtcggcctgg ggcgcgtggc tgcgcaaccc ggcgaacccg gccggcctca
     4801 cgtttcaggg ccgcgagatc gaccttgaag tattgttgcg ccaggcgctg ctcgacggaa
     4861 cgaatcctca aaaccctcac acctactggg gtgatatgga tcacatgagc cagcatatcg
     4921 tcgaggccgc cgacgtggcg gtgacggtgt ggttgagccg cgagcgcgta ttcgatcgca
     4981 tgactgaggc cgaacgcgcg caggtgatga actggctggc gcaggtggac ggcaaacaga
     5041 cgtacttcga caactggatt ttgttcccgg cgatggcgca gatggtgcgg atgaaactcg
     5101 gctacccggc ccctgaggcc gatctcgatg cacggctcga gcagatggcc gccttctatc
     5161 gcggcgatgg atggtacgcc gacggcccgg gcgacgaatt cgatctttac aacgcctggc
     5221 tgttcggctg gcattacctg ttgtgggcct ggatagacgg cgcccgccgc ccggatcacc
     5281 gccggttggt tttggatcgc gcccgcgcct ttctcaacgg ctttcaatat ttcttcggcg
     5341 cgaatggatc gtacccggcg tgggggcgtt cgattgtcta tcgcttctcg gcggtggcag
     5401 ccttccagac cgggcatttg ctgaacgtcg ccccgtccga ccccggcctc ctgcgccgcc
     5461 tctccagcgg ctgcattcgc tacttctacg agcgcggcct gctcgacccg ggcgcgcacc
     5521 acatccggca gggatttcac ggtgactttc ctcccgccgg cgagtcttac atctctccgg
     5581 gttcaccgta ctgggcctgt cacggacttt ttgcgctgac cttcgaccgt gacgacccgt
     5641 tctggacggc gaccgaagcg ccgctggccg tcgagcgtga agatttcgat ctggccctgc
     5701 ccgcgccggg tttcgtcctg tccggacggt gggccaccgg gcaggtgatc ctgctgaaca
     5761 gccggagtag tcaggccgag gagatcgtgc cggatgcggg tggggggcag ttgaccagcg
     5821 aggctgagct gatgacgcgc ttcgcgccgc ggcacgatta tcccgccaag tacggcaagc
     5881 tcgcctacag cacgcacttt cccttcaacg tcgcgcccgt gcccggctca tatgcgcccg
     5941 atgcgatggt ggctttgagt caggacggac agagattcgg ccatcgttcg accacgcgcg
     6001 ctggcggtgt tgcccccggc gtgatgtggt gcgagttcga cgaaatggtt gacgggcagc
     6061 cgcaaacgat acatctggcc gtcttattat ggaaggactt gcaggttcgc gtcgctctcc
     6121 ttcagcccac tctgccggtg cgcgcttttg aagcgccggg ggcgttgggg tgtgattgtg
     6181 cgtcgaagat tgtgcgccgc tccgacccgg acgctgggtg ggaatatgcc gaggcggacg
     6241 ggcgtgcgtt gggcgtccgg cgtctgttgg gctacgacgg tcagcaggcc agcgcgcctt
     6301 tcctgggcta ctccaacatc aatctcgcct atctctattc tgagcagcca atggtgtacg
     6361 aaacacgacc gagcgccgcg ctgcgcggtc tggccgccgt gagtcttttg cggcctgcgc
     6421 catttgatcc ggcg
//
LOCUS       DBNGWZ010000516        11179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1076740, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000516 DBNGWZ010000000
VERSION     DBNGWZ010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1076740"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="AC16CX_15780"
     CDS             complement(<1..1424)
                     /locus_tag="AC16CX_15780"
                     /inference="COORDINATES: protein motif:HMM:NF017727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HIR3014003.1"
                     /translation="MKVELLVSEWCPTCPAAEQVWREVAAERDFRFTVLDVAQYEGRE
                     LAQRLRIRTVPSIVVDGVLAGVGVQSLAEARQMVAGAPARARFSLLHAGVMLARAEGE
                     RRPLPTPMWRALTSSPHRPMFLLGALQIVATMLWWTADLAGRYAGWYAPLVWALPPSW
                     GHAYLMTYALFPLFIFGFTLTAVPNWTGRPIPRASWLAAAALLGLGVGLLYAGLVFSR
                     AILGAGVALLLAGWGVAWFALARNVAADYLRDRYAVGLVAMLAVGWLGAAGLGLYLLT
                     DEPLYAELSRRGGIWLFLLPLFLVVGHRLIPFFSSRVIRDYAIYRPQWSLPFLTAACV
                     AHFALEMLNLRQWSWTVDAPMAAWVGYLAVRWGLARSLRVKLLVMLHLSLVALAIALS
                     LYSIESAVALLGGAGLLGLAPQHVLVIGYFAATAVAMVSRVSLGHSGRALEADNLTWR
                     CFLGVLGVAALRLAADLAWVPAGAR"
     gene            complement(1522..1968)
                     /locus_tag="AC16CX_15785"
     CDS             complement(1522..1968)
                     /locus_tag="AC16CX_15785"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033293 - monocarboxylic acid binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl attachment domain-containing
                     protein"
                     /protein_id="HIR3014004.1"
                     /translation="MAEYRGCELPEDLYYDLDYIWARPEAGGRYRIGVTDPSQTMAGR
                     IQSVLIREVGRHRKRKQALAVVESGKWVGGIPAPFDGTVVERNEKVVENPLLINIAPY
                     TEAWIVVMQPDDPATALAHLATGEEAQRRLREWIDRYNVQCMRCAE"
     gene            complement(2048..2191)
                     /locus_tag="AC16CX_15790"
     CDS             complement(2048..2191)
                     /locus_tag="AC16CX_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014005.1"
                     /translation="MRLETPQPLKEEHAEFAHKLMLHAQTEEEVLYPAALLVGEHIKL
                     RLI"
     gene            complement(2282..3022)
                     /locus_tag="AC16CX_15795"
     CDS             complement(2282..3022)
                     /locus_tag="AC16CX_15795"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014006.1"
                     /translation="MDSGVPISAAVRPAILLEGLRKTYGSGVTAVEALKGVDMAVYPG
                     EVIGLIGPSGSGKTTLLKCLGAVIEPSAGRITLDDKVIYDGGWRVGDLRALRRDRIGF
                     IFQAPYLIPFLNATDNVALLPMLAGRANAAARARAVEMLAALDVAHRAAAQVAELSGG
                     EQQRVSIARALANQPPIILADEPTAPLDSQRALTVIRILNHLAREYRTAIIVVTHDEK
                     IIPTFRRIYYIRDGRTVEERGEGRDIGP"
     gene            complement(3023..4216)
                     /locus_tag="AC16CX_15800"
     CDS             complement(3023..4216)
                     /locus_tag="AC16CX_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014007.1"
                     /translation="MINLASRDIAHSFGRYLLTGLGLGLLIGVTLSMAGIYRGMVDDA
                     KALLNSWQADVWVVQQHTLGPFAESSNLHDDVYRGLLGMPGVADAGNVTFLTMQVKRG
                     DHDVRVMISGFEPGRPGEPAVQAAGRPVTRSHYEAIADVTTGFQVGERIHIRRHEYTV
                     VGLTRRMVSSSGDPMVFIPLKDAQEAQFLKDNEAIRNDRNRLAANPSLNRPGVPGLLA
                     AVEANQFSNHNVNAVLVRVQPGWDPRVVADNIKRWKYLQAYTGEDMEDILVAKLIATS
                     ARQISVFLVILALVSAAIVALIIYTMTIGKIKEIAVLKLIGTKNTTIAGMILQEALGL
                     GLIGFVIGKLAATLWGPHFPKYVVLLTEDAVRGFIATMAICAIASILGIRAALRVDPA
                     AAIGG"
     gene            complement(4213..5379)
                     /locus_tag="AC16CX_15805"
     CDS             complement(4213..5379)
                     /locus_tag="AC16CX_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3014008.1"
                     /translation="MKLKWKTAAAGGGIAVAVVALAWFATQRGPLAPVTVQTAPVSVS
                     ELQRSVFGIGTVEARYTYAIGPTQAGRVLRVLVDHGDRVKAGQLLAEIDPIDLEQRLS
                     AAEAALQRAHYGVVVAEAQVREAESRHRVASANAARYRDLAQRQFVSKEAADSRGNEA
                     DTAQAALEAARAALLGAQRDAERAQQERNGAARQLASLKLVSPVSGVVIAREAEPGNT
                     VVAGQAVVRIIDPDSVWVRARIDQSRAYGLQLGLPADVVLRSGGGTVIPAQVARVEIQ
                     SDAVTEEKIVAVSFVSPPGTLSLGDLAEVTIRHENGVRGFAIPTAAVTRSEQRTGVWQ
                     VVDGKTHFRALRLGVQTLDGRTQVIDGLAEGESVVVYSSAQLREGMSVRIRNAS"
     gene            complement(5594..6199)
                     /locus_tag="AC16CX_15810"
     CDS             complement(5594..6199)
                     /locus_tag="AC16CX_15810"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3014009.1"
                     /translation="MAMSARDVVTATIKREHYALEMVLAVLQRLIGDIAEQRAEPDFA
                     LFSAALYYIDDFPERCHHPKEDEYLFKALRRRTTRHDAVLDDLQAEHIRSAQMAGYLQ
                     RALVHYQGGAPGGLKLFGAAVDAYAGMLRDHMRKEDELLEQAPADLAEEDWKGIAAAF
                     DANDDPLFGKNRRDEFNKLYLRIINLLPRKMSMPLRGDHHE"
     gene            complement(6236..6388)
                     /gene="ccoS"
                     /locus_tag="AC16CX_15815"
     CDS             complement(6236..6388)
                     /gene="ccoS"
                     /locus_tag="AC16CX_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HIR3014010.1"
                     /translation="MDILYLLIPLSIMLVFAAGAVFWWAIGSGQFDDLEGPAHRVLTD
                     DDRPPR"
     gene            complement(6540..9092)
                     /locus_tag="AC16CX_15820"
     CDS             complement(6540..9092)
                     /locus_tag="AC16CX_15820"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3014011.1"
                     /translation="MSTITEVPFTAAARPAHCFHCGEQVADHGRLQVVVDGVARPMCC
                     AGCAAVARTIVENGLTDYYRSRSELPQRELAAPVVPDELRLYDIPEVQQTFAHGADAS
                     RRRAALLIEGITCGACAWLIEQRLQRMPGVAHVAVNLAARRAQVEWDPQRVQLSALLQ
                     AIADLGYRAQPFDPGRSDLVAARERRSLLWRLFVAGFGAMQVMMYAAPAYLAEGDMSR
                     DVEALMRWASLMLTVPVVAWAAGPFFTNAWRDLKARRVGMDVPVALGIAVAFIASAYA
                     TVRGAGEVYFDSISMFVFLLLGARYLEALARAKAAESQQRLVKHMPAVAERLAADGGM
                     TERVAVASLKADDRVLVAPGARIPADGKVVEGASATDETLLTGESRPVSKRAGDAVIG
                     GSINLKSPLTVQVTRVGQDTLLAGIVRLMDRAQAEKPRLAQLADRVAQWFVAALLAVA
                     AVTALAWYLIDPQRAPWVVVAVLVVTCPCALSLATPAALTAATGALHRLGMLVTRGHA
                     LETLARATHVVFDKTGTLTTGRLSLIGVMPVDGHNAGECLRLAAALERGSEHPIARAV
                     TAAAPRIAPLDVAGLRNILGEGIEARVEGMRVRIGSPAFVGALTRRPPPAEMLFTVSD
                     ITVVALGSEEGWMALFTFGDAPRPRARTLVRELQAHGLAVCMLSGDRHETAAHVAREL
                     GIETFVGEATPRAKLDFIRGLQAQGAIVAMVGDGVNDAPVLAQAQVSIAMGGGTELAQ
                     ASADMVLLGEDLDRLLEAVEVARKTMRVVRQNLAWAVAYNAVALPLAVAGLVTPLVAG
                     IGMALSSLAVVLNALRLASPRHARGPTPPKEFVGFGPDKLLMQNRQQEKSAG"
     gene            9248..9895
                     /gene="ccmA"
                     /locus_tag="AC16CX_15825"
     CDS             9248..9895
                     /gene="ccmA"
                     /locus_tag="AC16CX_15825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HIR3014012.1"
                     /translation="MFEARELEAARGGATLFRDLGFTLKPGGLLRVSGANGSGKTSLL
                     RILCGLLTPVAGEVRWQGENIHALREEYWKQLAYVGHANALKDDLTVMENLMVACALA
                     GLSLTAARARAALERFGLAGRGGLPVKALSQGQRRRAALARLLASETLPLWILDEPFA
                     ALDAAAVELVQTVAGEHLTRGGMVVLTTHQEVRIQAPSVTDINLGDGRVDVRPSP"
     gene            complement(10030..10983)
                     /locus_tag="AC16CX_15830"
     CDS             complement(10030..10983)
                     /locus_tag="AC16CX_15830"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3014013.1"
                     /translation="MQTQPLDIGVDIAKDEVVVACAAATFVPRAIPNRRAGLGAWLKS
                     LPKGSRIGLESTSSYHELLADLAHRQGLSVFVLNPRDTHHYAKGVGARAKTDRVDAQI
                     IARFLAREYPHLHPYQPPTPEQRRIDRLLKRRAKLSAIRGTLRQSLKELESCRAEVKA
                     VLAKFDALIKKIDLTLAALSAACPERFQTQQRLQSVVGIGPLVSMSLTNLLQRVPFRN
                     ADAFVAFLGYDTRPEDSGQKHGRRRLSKRGPAEPRRLLYNAAMAASQCHPWKPIYQHY
                     CDKGLSSTAAFCIIARKIARTAWSIFKYQTTFDPQRLTCQP"
BASE COUNT         1878 a         3846 c         3687 g         1768 t
ORIGIN      
        1 cgcgccccgg cgggaaccca cgccagatcg gccgcgaggc gcagcgcggc cacgccgagc
       61 acaccgagga aacaacgcca ggtcaggtta tcggcctcga gagcccggcc cgagtgcccc
      121 agcgagacgc gcgacaccat cgccacggcg gttgccgcga aatagccgat gacgagaaca
      181 tgctgcggcg cgaggccgag cagccccgcg ccgccaagca gcgcgacggc gctctcgatg
      241 gagtagagcg acaaggcgat cgcgagcgcc acgagtgaaa ggtgcagcat cacgagcagc
      301 ttcacgcgca ggctccgggc aagcccccag cgtaccgcga gataaccgac ccacgcggcc
      361 atcggcgcgt ccaccgtcca gctccattgc cgaaggttca gcatttcgag cgcaaaatgc
      421 gcgacgcacg ccgccgtcaa aaaaggcaac gaccattgcg ggcggtagat cgcgtaatcc
      481 cggatgacgc ggctggaaaa gaacgggatc aggcgatggc cgacgaccag gaacaacggc
      541 agcaggaaga gccagatgcc gccgcgacgt gaaagctcgg cgtagagcgg ctcgtccgtg
      601 aggagataca ggcccaggcc ggcggcgccg agccagccaa ccgcgagcat ggcgaccagg
      661 cccaccgcgt agcggtcccg caggtaatcg gcggcgacgt tgcgcgcaag cgcgaaccac
      721 gcgacacccc agcccgcgag cagcagcgcg acgccggcgc ccaggatcgc gcggctgaac
      781 acaagtccgg cgtagagcag ccccacgccg agcccgagca acgccgccgc ggcgagccag
      841 ctcgcgcgcg ggatcggccg cccggtccag ttcggcaccg ccgtcaacgt gaagccgaaa
      901 atgaacaggg gaaacagcgc gtacgtcatc aggtaggcgt ggccccagga cggcggcaaa
      961 gcccacacaa ggggcgcgta ccagcccgcg tagcggccgg cgaggtctgc ggtccaccac
     1021 agcatggtgg ccacgatctg cagcgcgccg agcagaaaca tcggccggtg cgggctcgaa
     1081 gtcagcgcgc gccacatcgg cgtcggaagc ggccgccgct cgccttctgc acgcgcaagc
     1141 atcaccccgg cgtgcaggag ggagaatctc gcgcgggcgg gagcgccggc taccatctgc
     1201 cgcgcttcag cgaggctttg gacaccaact cccgcaagca ccccgtccac cacgattgag
     1261 gggaccgtcc ggatgcgcag ccgttgcgca agctcgcgcc cctcgtactg cgcgacgtcg
     1321 aggaccgtga agcggaagtc gcgctcggcc gcgacttcgc gccacacctg ctccgccgcc
     1381 gggcaggtgg gacaccattc ggagacgagc agctcgactt tcatgactgg aatccgttag
     1441 cggtcagcgg tcagcggtca gcggaaagag caaaaccgtg cggcgccggc tcaccgctta
     1501 cggcttaccg tttaccatct ctcactcagc gcagcgcatg cattgcacgt tgtagcggtc
     1561 gatccactcg cggagcctcc gctgcgcctc ctcgccggtc gcgagatgcg cgagcgccgt
     1621 tgcaggatcg tcgggctgca tgaccacgat ccacgcctcg gtataaggcg cgatgttgat
     1681 gagaaggggg ttttccacga ccttctcgtt gcgctcgacg accgtgccgt cgaacggcgc
     1741 cgggatgccg cccacccatt tccccgactc gacgaccgcg agcgcctgct tgcgcttgcg
     1801 gtgcctgccg acctcccgga tcagcacgct ctggatccgc ccggccatgg tctgcgacgg
     1861 atcggtgacg ccgatgcgat agcggccgcc cgcctccggc ctcgcccaga tgtaatcgag
     1921 gtcgtagtag aggtcctccg gcaactcgca tccgcggtac tcggccatcg ctatttcggc
     1981 ttgggcggcg agcgcaatcg aacggtcaac accagcaacg cgagccgatc cgcgccggct
     2041 acgccatcta aatgagcctc agcttgatgt gctcgcccac caggagcgcc gccgggtaga
     2101 gcacttcctc ctcggtctga gcgtgcagca tgagcttgtg cgcgaactcg gcgtgctctt
     2161 ccttcagcgg ctgcggtgtt tccagcctca tcattcactc ccggttgcct gtgcatcgct
     2221 ttcaaaacag aatagagctt cctccggacc ggcttttgat tcagatcaaa cgccgtgcgc
     2281 gtcacggccc gatatcccgc ccctcgcccc gctcctccac ggtgcggcca tcgcggatgt
     2341 agtagatgcg gcggaaagtc gggatgattt tctcgtcgtg cgtgacgacg atgatcgccg
     2401 tgcggtactc gcgcgccaga tgattgagaa tccggatgac ggtgagggcg cgctggctgt
     2461 cgagcggcgc ggtcggctcg tcggcgagga tgatcggcgg ctggttcgcc agggcgcggg
     2521 cgatcgacac gcgctgctgc tcgccgccgg acaattccgc aacctgcgcc gcggcgcgat
     2581 gcgcgacgtc gagcgcggcg agcatctcca ccgcgcgggc gcgggcggcg gcattggctc
     2641 ggcccgcgag catgggcagc agagcgacgt tgtcggtcgc gttcaggaac ggtatcagat
     2701 agggcgcctg aaaaatgaag ccgatgcggt cgcggcgcaa cgcgcgcaga tcgcccaccc
     2761 gccagccgcc gtcatagatg actttgtcgt cgagcgtgat gcgtccggcg ctcggctcga
     2821 tcaccgcgcc caggcacttg aggagcgtgg tcttgccgga gccgctcggt ccgatcagcc
     2881 cgatcacctc ccccgggtag accgccatgt ccacgccttt cagcgcctcc accgccgtca
     2941 cgcccgaacc gtaggtcttg cgcaggccct cgagcaggat cgcgggccgc accgccgcgg
     3001 aaatgggaac accgctgtcc actcatcctc cgatcgcggc cgccggatcc acgcgcagcg
     3061 ccgcgcggat gccgaggatg ctggcgatgg cgcagatcgc catggtcgcg atgaatccgc
     3121 gcacggcgtc ctcggtgagc agcaccacgt acttcgggaa gtgcgggccc cacagcgtcg
     3181 ccgccagctt gccgatgacg aacccgatca gcccgaggcc gagcgcttcc tgcaggatca
     3241 tgccggcgat cgtggtattt ttcgtgccga tcagcttcaa caccgcgatt tccttgatct
     3301 tgccgatcgt catcgtgtag atgatcagcg cgacgatggc ggcgctcacc agggcgagga
     3361 tcaccaggaa cacggagatc tggcgggccg aggtggcgat cagcttggcg accaggatgt
     3421 cctccatgtc ctcgcccgtg tacgcctgca ggtatttcca gcgcttgatg ttgtcggcca
     3481 cgacgcgcgg gtcccagccc ggttgcaccc gcaccaggac ggcgttgacg ttgtggttgg
     3541 agaactggtt ggcctcgacc gccgccagca gccccggcac gccggggcgg ttcaacgacg
     3601 ggttggcggc aagccggttg cggtcgttgc ggatcgcctc gttgtccttg aggaactgcg
     3661 cctcctgcgc gtccttgagc gggatgaaca ccatcggatc gccgctcgac gacaccatcc
     3721 ggcgcgtcag tccgaccacg gtgtactcat ggcggcggat atggatccgc tcaccgacct
     3781 ggaaaccggt cgtgacgtcg gcgatcgcct cgtaatgcga ccgagtcacc ggtcgccctg
     3841 ccgcctgcac cgcgggctct ccgggccgcc cgggctcgaa acccgaaatc atcacgcgca
     3901 catcgtggtc gcctcgcttc acctgcatgg tgagaaaggt gacattcccg gcatcggcca
     3961 ccccgggcat gccgaggagg ccgcggtaca cgtcatcgtg caggttggaa gactccgcga
     4021 acggcccgag cgtgtgctgc tgcacgaccc agacatccgc ctgccagctg ttcagcagcg
     4081 ccttggcatc gtccaccatg ccgcggtaga tgccggccat gctgagcgtg acgccgatca
     4141 gcagccccag cccgaggccc gtcagcaggt agcgcccgaa gctgtgcgcg atgtcacggc
     4201 tggcgaggtt gatcatgacg cattcctgat cctgacgctc atgccttcgc gcaactgcgc
     4261 gctgctgtag accaccacgc tctccccctc cgccagcccg tcaatcactt gcgtcctgcc
     4321 gtcgagcgtc tgcacgccga gcctgagcgc gcgaaaatgc gtcttgccgt ccacgacctg
     4381 ccacaccccc gtgcgctgct cgctgcgcgt gaccgcggcg gtgggaatgg caaacccgcg
     4441 cacgccgttc tcgtggcgga tcgtgacctc cgccagatcg cccaacgaga gcgtgcccgg
     4501 aggggaaacg aacgacacgg ccacgatttt ttcctctgtg acggcatcgc tctggatctc
     4561 gacgcgcgcg acttgcgccg ggatcaccgt gcccccgccg gaacgcagca ccacatcggc
     4621 gggaagtcca agctgcaagc cgtatgcccg ggactggtcg atgcgcgcgc gcacccacac
     4681 gctgtccgga tcgatgatgc gcaccaccgc ctgtcccgcg accaccgtgt tgccgggctc
     4741 ggcttcgcgg gcgatgacca cgccgctcac ggggctcacg agtttcaggc tggcgagctg
     4801 ccgggcggcg ccgttgcgtt cctgctgcgc gcgctcggca tcgcgctgcg cgcccagcag
     4861 cgcggcgcgc gccgcctcga gcgccgcctg cgcggtgtcg gcctcgttgc cacggctgtc
     4921 cgccgcttcc ttgctcacga actggcgctg cgcgagatcg cgatagcgcg ccgcgttggc
     4981 gcttgcgacc cggtgccggc tctcggcctc gcgcacctgt gcttcggcca cgaccacgcc
     5041 gtagtgcgcg cgctgcagcg cggcctccgc tgcgctcaag cgctgctcca gatcgatggg
     5101 atcgatctcg gcgagcaatt ggcccgcctt gacccggtcg ccatggtcca ccagcacccg
     5161 cagcacgcgg ccggcctgcg tcggcccgat ggcgtaggtg tagcgtgctt cgaccgtgcc
     5221 gatgccgaac acgctgcgct gcagctcgga aacgctcacc ggcgccgttt gcactgtgac
     5281 cggcgccagc ggacctcgct gtgtcgcgaa ccacgcgagc gccaccaccg ccacggcgat
     5341 gccgccgccc gccgccgctg ttttccactt cagcttcata cgcactccca ccgcggcggc
     5401 tctagcagag cgaccgcgac catcccatca ggctggatct gaaacgacat tggcccatga
     5461 tagggtcatg ggcaaagcgg ctgttgacgt agatcaacct aggccggcgc acattcctgc
     5521 gcacagacta gggggcacgg ctagggcggg aatcttgctc catcaggccg gcaagccgcc
     5581 tccgctgcgc ccgttattcg tgatggtcgc cccgcaacgg catgctcatc ttgcgcggca
     5641 gcaggttgat gatgcggagg tagagcttgt tgaattcgtc gcgccggttc ttgccgaaca
     5701 acggatcgtc attggcgtcg aacgccgccg caatcccttt ccagtcctct tcggcgagat
     5761 ctgccggggc ctgttccagc aattcgtctt ccttgcgcat gtgatcgcgc agcatgcccg
     5821 cgtaggcgtc gaccgcggcg ccaaacagct tcaacccccc cggcgctccg ccctggtagt
     5881 gcaccagtgc gcgctgaagg tagccggcca tctgcgcgct gcggatatgc tcggcctgca
     5941 ggtcatccag caccgcgtcg tgccgcgtgg tacggcggcg cagcgccttg aacaggtatt
     6001 cgtcctcctt cggatgatgg cagcgctcgg ggaagtcgtc gatatagtag agcgccgcag
     6061 aaaacaacgc gaaatccggc tcggcccgct gttcggcgat atcgccgatc aaccgctgca
     6121 gcaccgccag caccatttcc agcgcatagt gctcccgctt gattgtcgcc gtaactacgt
     6181 ctctggcgct catcgccata ccgtcctgtc gcctttccag gttcatcccc ccgcctcacc
     6241 ggggaggccg gtcgtcgtcc gtcaggacgc ggtgcgccgg tccctcgagg tcgtcgaact
     6301 gcccggaccc gatcgcccac cagaacaccg cgccggccgc gaacaccagc atgatcgaaa
     6361 gcggaatcag cagatagagg atgtccatgg tcaggcctcc gcgagccgcc agcagcctat
     6421 cggacttggc ggcccgaata ggctgctaac agcaaaaccg gctgacggtt caacaaaaaa
     6481 cccggtccaa gcggtcaaac ctggagataa tcggggaggc atccctatgt ccgtcctttt
     6541 catccagcac ttttttcctg ctggcggttt tgcatcagga gtttgtcggg gccaaatccg
     6601 acaaactcct tgggcggcgt cggcccgcgc gcgtgccggg gactcgccag ccgcagcgcg
     6661 ttgagcacga cggcaagcga cgacaacgcc atgccgatgc cggcgacgag cggcgtgacc
     6721 agccccgcaa cggcgagcgg cagggccacc gcgttgtagg cgacggccca cgccaggttc
     6781 tggcggacca cccgcatcgt cttgcgcgcg acctccaccg cttcgagcag ccggtcgagg
     6841 tcctcaccga gcagcaccat atccgcgctg gcttgcgcga gctcggtgcc gccgcccatc
     6901 gcgatcgata cctgcgcctg cgccagcacc ggcgcatcat tcacgccgtc gcccaccatt
     6961 gccacgatcg cgccctgcgc ctgcagcccg cggatgaaat cgagcttggc ccggggtgtc
     7021 gcctcgccca cgaacgtctc gatgccgagc tcgcgggcca cgtgtgcggc ggtttcgtgc
     7081 cggtcgcccg acagcataca taccgcaagg ccatgcgcct ggagctcgcg cacgagggtt
     7141 cgcgcgcgcg ggcgtggcgc atccccgaag gtgaacagcg ccatccaccc ttcttcgctt
     7201 ccgagcgcga ctaccgtgat gtcgctgacg gtgaacagca tctcggcagg tggcggccgg
     7261 cgcgtcaacg cgccgacgaa cgccgggctc ccgatgcgca cccgcattcc ctcgacgcgc
     7321 gcctcgatgc cttcgcccag aatgttgcgc agcccggcga cgtcgagggg ggctattcgg
     7381 ggtgcggccg ccgtgacggc gcgggcgatc gggtgctcgg aaccgcgctc gagcgcggcc
     7441 gcgagccgca ggcactcccc ggcattgtgc ccgtccacgg gcatcacgcc gatgagcgag
     7501 agccggccgg tggtcagcgt gccggtcttg tcgaaaacga cgtgagtagc gcgcgccagc
     7561 gtttcgagcg cgtggccgcg cgtcaccagc atcccgagcc ggtgcagcgc gccggtcgcg
     7621 gccgtgagcg cggcgggcgt cgcaagcgaa agcgcgcacg ggcaggtcac caccagcacg
     7681 gcgaccacta cccacggcgc ccgctgcggg tcgatcaggt accacgccag cgcggtcacc
     7741 gcggcgaccg cgagcagcgc ggccacgaac cattgcgcga cgcggtcggc cagttgggcg
     7801 aggcgcggct tctccgcctg cgcgcggtcc atgagccgga cgatgccggc gagcagcgtg
     7861 tcctgcccga cgcgggtcac ctgcacggtg agcgggctct tgagattgat gctgccgccg
     7921 atcaccgcat cgccggcgcg tttggagacg gggcgcgatt ctccggtgag cagcgtctcg
     7981 tcggtcgcgc tggcgccttc cacgaccttg ccgtccgcgg gtatccgcgc gcccggcgcg
     8041 accagcaccc ggtcgtcggc cttgaggctc gccaccgcca cgcgctcggt catccccccg
     8101 tccgccgcga gccgctccgc cacggccggc atgtgcttca ccagccgctg ctgcgattcc
     8161 gcagccttcg cccgcgccag cgcctcgagg tagcgcgccc cgagcaggag gaagacgaac
     8221 atcgagatcg agtcgaaata gacttcaccc gcccctcgca ccgtcgcgta ggcgctcgcg
     8281 atgaaagcga cggcgatgcc gagcgccacc ggtacgtcca tgccgacgcg gcgcgccttg
     8341 aggtcgcgcc aggcgttggt gaagaacggg cccgcggccc acgccacgac cgggaccgtc
     8401 agcatcaggc tcgcccaccg catcagcgcc tcgacgtcgc ggctcatgtc gccttccgcg
     8461 agataggccg gcgcggcgta catcatgacc tgcatcgcgc cgaagccggc gacgaacagc
     8521 cgccacagca acgagcggcg ctcgcgagcc gcgaccagat cgctgcggcc gggatcgaac
     8581 ggctgcgcgc ggtagccgag atcggcgatc gcctgcagca gcgcgctcaa ctgcacgcgc
     8641 tgcggatccc attccacctg cgcgcgccgc gccgcgagat tgacggcgac gtgcgccacg
     8701 ccgggcatcc gctgcagccg ctgctcgatc agccacgcgc acgcgccgca ggtgatgccc
     8761 tcgatcagca gcgcggcccg gcggcgcgat gcatcggcac cgtgcgcgaa ggtttgctgc
     8821 acctccggta tgtcgtagag gcgaagctcg tccgggacga cgggcgccgc aagctcccgt
     8881 tgcggcagct cgctgcggct gcggtagtag tccgtgagac cgttctcgac tatggtccgc
     8941 gccaccgccg cgcagccggc gcagcacatc ggtcgagcca cgccgtccac caccacctgg
     9001 aggcggccgt gatcggccac ctgctctcca caatgaaagc aatgcgcggg gcgggccgcc
     9061 gccgtgaagg ggacttcggt gatcgtgctc atcgggacgg gaacaggact cgttgacaac
     9121 gatgctaagc ccgggcccgg agccggagtt tgacctaaat caatccctgc tatagcgtga
     9181 cgcacccatg cgagcagccg gaacctgcat caggagaccg gctccttcaa cagaccctgg
     9241 cgatcccatg ttcgaggcac gcgaactcga ggcggcacgc ggcggcgcga cgctgttccg
     9301 cgatctcggc ttcacgctca agcccggcgg cctgctgcgc gtgtccggcg ccaacggcag
     9361 cggcaaaaca agcctgctgc gcatcctgtg cgggctgctc acgccggtcg ccggcgaggt
     9421 gcgatggcag ggcgagaaca tccacgcgct gcgcgaggaa tactggaagc agctcgccta
     9481 cgtcgggcac gcgaacgcgc tgaaagacga tctcacggta atggaaaatc tcatggtcgc
     9541 ctgtgcgctg gcggggctga gcctgacggc ggcgcgtgca cgggccgcgc tggagcgctt
     9601 cgggctcgcc gggcgtgggg ggcttccggt caaggcgcta tcgcaagggc agcggcggcg
     9661 ggccgcgctc gcgcgcctcc tggcaagcga aacgctgccg ttatggatac tcgacgagcc
     9721 gttcgccgcg ctggacgcgg cggcggtgga actggtgcag accgtcgcgg gagaacacct
     9781 cacgcgcggc ggcatggtgg tcctgaccac ccatcaggag gtgcgcatcc aggcgccctc
     9841 ggtgacggac atcaacctag gagacggtcg ggttgacgtc cgaccttctc cttagagagc
     9901 ctgacagaat gaagcagcgc tacttgcttc ccctttatca aggccgagca gcccgcaggg
     9961 ccgccgtggt tgagacgaga cggtcatttt tctgcttttc ggggcccctg agagattcta
    10021 tggttctggt caaggctggc aggtgaggcg ttgcggatca aaggtggttt ggtacttaaa
    10081 gatcgaccat gccgtgcgtg cgatcttgcg ggcgatgatg cagaaggcag cagtgctgct
    10141 gagtcctttg tcgcaatagt gttggtagat cggcttccag gggtggcatt ggctggcggc
    10201 catggcggcg ttatacagta gacgtcgagg ttcggcggga cctcgcttgc tcaatcgtcg
    10261 gcggccgtgc ttttggcccg agtcctcggg gcgcgtgtcg tagcccaaga aggcgacgaa
    10321 ggcatcggca ttgcggaaag gcacgcgctg cagcaggttg gtgaggctca tgctcacgag
    10381 ggggccgatg ccgacgacgg attggaggcg ctgctgggtc tggaagcgtt cggggcaggc
    10441 agcgctcaga gctgccaagg tgaggtcgat ctttttgatc agggcatcga acttggccaa
    10501 taccgccttg acctcggcgc ggcagctctc cagttccttc aagctctggc gcaacgtgcc
    10561 tcggatggcg ctgagcttgg cgcgccgctt gagcagccga tcgatgcgac gctgctcagg
    10621 ggtaggcggt tgataaggat gcaagtgagg gtattcgcgg gcgagaaagc gggcgatgat
    10681 ttgggcgtcc acgcgatcgg tcttggcgcg tgcgccaacg cccttggcgt aatggtgggt
    10741 gtccctggga ttgagcacga atacgctcaa gccctggcgg tgggccagat cggcaagcag
    10801 ttcatgatag gaactggtgg attccagccc aatgcgactg cctttcggca gggatttgag
    10861 ccaggcgccg agcccggcgc gacggttggg gatggcacgc gggacaaaag tggctgcggc
    10921 gcaggccact acgacctcgt ccttggcgat atccacgccg atgtccaggg gttgagtttg
    10981 cataggtgtg ctctccgggt ataaggtgaa aaatgctggg gtggacacac accgacgtta
    11041 gcttgcgatc aaatcggcgg tcagggtgat ggtgcaccat gccgctagat tcctcatcga
    11101 cgttggtggt gtgggtggaa gatctctctc agagtctgtc cgcaacgcgg cagataaccg
    11161 ccgaagtctc tccacccca
//
LOCUS       DBNGWZ010000517         5782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_823641, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000517 DBNGWZ010000000
VERSION     DBNGWZ010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_823641"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /gene="smpB"
                     /locus_tag="AC16CX_15835"
     CDS             complement(<1..281)
                     /gene="smpB"
                     /locus_tag="AC16CX_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0070929 - trans-translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR3014014.1"
                     /translation="MSIVQNRKAFHDYFIEQRFEAGIALEGWEVKAIRAGRAQLKEAY
                     VIVSNGELFLTGCHVSPLPAASTHFIPDPTRTRKLLLHAAEISRLIGSV"
     gene            361..792
                     /locus_tag="AC16CX_15840"
     CDS             361..792
                     /locus_tag="AC16CX_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HIR3014015.1"
                     /translation="MTEVNRSVLVGFTPGQMFTLVDAVEDYPKFLPWCGGATVIHRDE
                     GITRAALLINYHGIKHSFTTENTKSAPSQMLIRLVEGPFRRLDGSWRFTGLAGRGCKV
                     ELRLQYEFSSRVLEKLVGPVFNHIASTLVDAFARRAEQLYG"
     gene            821..1129
                     /locus_tag="AC16CX_15845"
     CDS             821..1129
                     /locus_tag="AC16CX_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HIR3014016.1"
                     /translation="MSGQSQIKIEVVYALPEKQVLLEVEVDAGTNVRQAIERSGILSR
                     FPEIELARGRVGIFGRPVHLDALLNDGDRVEIYRPLMADPKQARQGRAQQSPRQGRGD
                     "
     gene            complement(1182..1640)
                     /locus_tag="AC16CX_15850"
     CDS             complement(1182..1640)
                     /locus_tag="AC16CX_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014017.1"
                     /translation="MKAIVAFLLVLAAVPAHGQLLKCVGKDGKVEYAAQCPPGTKELQ
                     TGIRSRSAPGGPSPSAKTKQPSLAEQEAAFRKRQVEQSEAQGKQQKEAAEAAQKQRAC
                     EEARNYLAGLEAGARITRVDPKTGERVFLEDAGRAQEIERARQSVQANCK"
     gene            1776..3242
                     /gene="guaB"
                     /locus_tag="AC16CX_15855"
     CDS             1776..3242
                     /gene="guaB"
                     /locus_tag="AC16CX_15855"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3014018.1"
                     /translation="MRVVQKALTFDDVLLIPAHSNVLPREVSLTSKLTRGITLNIPVV
                     SAAMDTVTEARLAIAIAQEGGVGIIHKNMPPKSQAAEVTKVKRFESGIVKDPITITPS
                     MKVRDVLSLTQQHKISGLPVINGSGRVVGIVTNRDLRFETKLDQPVKNIMTPRERLVT
                     VREGAGREEAMALMHKHRLERVLVVNRKFELRGLVTVKDILKSTEHPSACKDSLGRLR
                     VGAAIGVGEGTEERAEALVEAGADVIVVDTAHGHSKNVLDRVKWVKRNFPRTQVIGGN
                     IGTAAAAKALVDHGADGVKVGIGPGSICTTRIVAGVGVPQISAIANVAKGLLKSDVPL
                     IADGGIRYSGDIAKAIAAGAHVVMIGGLFAGTEESPGEIELYQGRSYKSYRGMGSLSA
                     MQQGAADRYFQDEEEPDTDKLVPEGVEGRVPYKGPLASLVQQLTGGLRSSMGYLGCST
                     IEEVHSRAEFVEITTAGIRESHVHDVQIVKEAPNYRVE"
     gene            3335..4900
                     /gene="guaA"
                     /locus_tag="AC16CX_15860"
     CDS             3335..4900
                     /gene="guaA"
                     /locus_tag="AC16CX_15860"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3014019.1"
                     /translation="MQHEKILILDFGAQYTQLIARRVREAHVYCEIHPYDVADQFIRA
                     FNPRGIILSGGPASVWEGGTPRAPQAVFELGVPVLGICYGMQTMAAQLGGKVEMGRVR
                     EFGYAEVRARGHSALLKDIQDRVNAEGHGLLDVWMSHGDKVNELPPGFKVIASSAACP
                     IAGMADEGRKLYGVQFHPEVTHTLQGRAILERFVLETCGLAGDWSMPNFIEEAIGRIR
                     SEVGPEEVVLGLSGGVDSSVAAALIHRAIGKQLTCVFVDHGLLRLNEAEQVMNTFAGH
                     LGASVIHVDARDQFMKQLKGVADPEEKRRIIGREFIEVFQREAFRLPQVKWLAQGTIY
                     PDVIESAGARTRKAHSIKSHHNVGGLPDTLHLKLLEPLRELFKDEVRELGTALGLPRD
                     IVFRHPFPGPGLGVRILGEVRAEYADLLRRADAIFIDELRESGWYDKTAQAFAVFIPV
                     KSVGVMGDGRTYEHVVALRAVQTQDFMTAQWAELPHGLLSKVANRIINEVRGINRVVY
                     DISSKPPATIEWE"
     gene            5001..5465
                     /gene="tadA"
                     /locus_tag="AC16CX_15865"
     CDS             5001..5465
                     /gene="tadA"
                     /locus_tag="AC16CX_15865"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR3014020.1"
                     /translation="MSDEIFMRKALSLAREAAAAGEVPVGAVVVKEGAVVGCGYNRPV
                     SRRDPTAHAEVIALRDAAENIGNYRLSGCELYVTLEPCAMCAGAIIHARISRVVYGAA
                     DPKAGACGSVVNLFEEGRLNHHAVIAGGVMAEEAGKLLQDFFSARRQTSSHA"
     gene            5560..>5782
                     /locus_tag="AC16CX_15870"
     CDS             5560..>5782
                     /locus_tag="AC16CX_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014021.1"
                     /translation="MGELNGSYCTPRGKHIIRAKIGAGQPVNAVFVERRPTGEIYNPE
                     LAERFPGRDWILTRILWLSGCERGYNRLGK"
BASE COUNT         1058 a         1786 c         1929 g         1009 t
ORIGIN      
        1 tcgacgctgc cgatgagccg gctgatctca gcggcgtgca gcaggagctt gcgcgtgcgc
       61 gtcgggtcgg gaatgaagtg cgtagaagcg gcaggcaacg ggctcacgtg gcagccggtg
      121 aggaaaagct cgccgttgct cacgatcacg tacgcttctt tcagctgggc gcgacccgcg
      181 cgtatcgcct tgacctccca gccttcgagc gcgatccccg cttcgaagcg ttgctcgata
      241 aagtaatcgt ggaaggcctt cctgttctgg acgatgctca tgcctggacc gcttctccgg
      301 tcattttaca gcgggacgcg gctgatattc ctttcccgga acgccggggg cggggaaccg
      361 atgactgaag tcaaccgctc ggtgctggtc ggattcacgc ccgggcagat gttcaccctg
      421 gtggacgcag tggaggatta cccgaagttc ctgccgtggt gcggcggcgc caccgtgatc
      481 catcgcgacg agggcatcac gcgcgcggcc ctactcatca attaccatgg aatcaagcac
      541 agcttcacca ccgaaaacac gaagagcgcg ccgtcacaga tgctgatccg gctggtggaa
      601 gggccgttcc ggcgcctcga cggcagctgg cgcttcaccg ggcttgccgg ccgcggctgc
      661 aaggtcgagc tgcggctgca atacgagttt tcgagccgcg ttctggagaa actggtcggg
      721 ccggtattca atcacatcgc cagcacgctg gtggacgcat tcgccaggcg cgccgagcag
      781 ctttatggct gaagggacag tgaatagtga gtggcgagtc gtgagtggac aatcccaaat
      841 caagattgag gtcgtttatg ccctgccgga aaagcaggtt ttgttggagg tggaagtgga
      901 cgcggggacg aacgtgcggc aagccatcga gcgttccggc atcctgtcgc ggtttccgga
      961 gatcgagctt gcgcgcggac gcgtgggcat attcggcagg cccgtgcacc tggacgcgct
     1021 gctgaacgac ggagaccggg tggagattta tcgtccgctg atggcggacc ccaagcaagc
     1081 gcgccagggg cgcgcgcaac agtcaccgcg ccaggggcga ggcgattagg tgtttgttat
     1141 acccgccttc gcggggatga cgaccgcctg ccgtgcggtc gtcacttgca gttggcctgg
     1201 acggactggc gggcgcgctc gatctcttgc gcgcggccgg catcttccag gaagacgcgc
     1261 tctccggtct tgggatcgac ccgcgtaatg cgggcgccgg cctcaagtcc cgcgagatag
     1321 ttgcgggcct cttcgcaggc gcgctgcttc tgggccgctt cggccgcttc tttctgctgc
     1381 ttcccctgcg cttccgactg ctcgacttgc cgcttcctga atgccgcctc ctgctccgcg
     1441 agggagggct gcttcgtctt ggcgctgggt gacgggccgc cgggcgcgct cctgctcctg
     1501 atcccggtct gcagctcctt ggtgccgggc gggcactgcg cggcgtattc gaccttgcca
     1561 tccttgccga cgcatttcag caactggcca tgggcgggaa cagcggcgag caccagcagg
     1621 aacgcaacga tggctttcac gggatcacct ctccaatctc ggttcgtcgg cacgacgtgc
     1681 cattttgtcc agtcaggtca atcaattaac gcggcaactc gtttacggtt gacgatcgca
     1741 tgcgtataat acgttttttg cgaagggtag aaaacatgcg tgtggtgcag aaagccctca
     1801 ccttcgacga tgttctgctg atccccgccc actccaacgt cctcccacgc gaagtcagtc
     1861 tcacgagcaa gctcacccgc ggcatcaccc tcaatattcc cgtggtttcg gccgcgatgg
     1921 acaccgtgac cgaggcgcgg ctcgcgatcg ccatcgcaca ggaggggggg gtcggcatca
     1981 tccacaagaa catgccgccc aagtcacagg ccgcggaagt gaccaaggtc aagcgtttcg
     2041 aaagcgggat cgtcaaggac ccgatcacca tcacgcccag catgaaggtg cgggacgtgc
     2101 tcagcctcac ccagcagcac aagatctccg ggctccccgt catcaacggc tcaggacgcg
     2161 tggtgggcat cgtcaccaac cgcgacctgc gcttcgagac caagctcgat cagccggtca
     2221 agaacatcat gaccccgcgc gagcggttgg tgacggtacg ggagggcgcg ggccgcgagg
     2281 aggccatggc gctcatgcac aagcatcgcc tggaacgggt gctggtggtg aaccggaagt
     2341 tcgagctgcg cggcctggtc actgtcaagg acattctcaa gtccaccgag caccccagcg
     2401 cgtgcaagga cagcctgggg cggctgcgcg tcggcgccgc gatcggcgtg ggcgaaggga
     2461 ctgaggagcg cgccgaggcg ctggtcgagg ccggcgcgga tgtcatcgtg gtggacacgg
     2521 cccatggtca ttcgaagaac gtgctcgacc gcgtgaaatg ggtcaagcgc aacttcccca
     2581 ggacccaggt cattggcggc aatatcggca ccgcggccgc cgcgaaagcg cttgtggatc
     2641 atggcgcaga cggcgtgaag gtcggcatcg ggccgggatc gatctgcacc actcgcatcg
     2701 tcgccggcgt cggcgtgccg cagatcagcg ccatagccaa cgtcgccaag ggcctgctca
     2761 agtcggacgt gccgctcatc gccgacggcg gcatacgcta ctcaggtgac atcgcgaagg
     2821 caatcgccgc cggcgcgcac gtggttatga ttggcggtct gttcgccggc accgaggagt
     2881 cgccgggcga aatcgagctc taccaggggc gttcctacaa atcgtaccgc ggaatggggt
     2941 cgctttccgc catgcagcag ggcgccgccg accgctattt ccaggacgag gaggagccgg
     3001 acaccgacaa actcgtgccc gagggcgtcg agggaagggt gccgtacaag gggccgctcg
     3061 cgtccctcgt ccagcaactc actggcgggc tgcgctccag catgggctat ctcggctgct
     3121 ccaccatcga ggaagtgcac agccgggccg agttcgtgga aatcaccacc gccggcatcc
     3181 gcgagtccca cgtccacgac gtgcagatcg tgaaggaagc gcccaactac cgagtcgagt
     3241 gaggggtgag gagtgaagag tgaggagtaa cggctttaac cggggttggt cggctttccc
     3301 cctcactcct tactcctcac acctcacctg acgcatgcag cacgagaaga tcctcatcct
     3361 cgacttcggc gcgcagtaca cgcagttgat cgcgcgccgg gtgcgggaag cccacgtcta
     3421 ctgcgagatc cacccctacg acgtggccga ccagttcatc cgcgcattta acccgcgcgg
     3481 catcattctt tcgggcgggc cggcctcggt atgggagggc ggcacgccgc gcgccccgca
     3541 ggcggtgttc gagctcggcg tgccggtgct gggcatctgc tacggcatgc agaccatggc
     3601 ggcgcagctg ggcggcaagg tcgaaatggg tagggtgcgg gaattcggct atgcagaggt
     3661 cagagcgcgc ggccactctg ccctgctcaa ggacatccag gaccgggtca atgcggaagg
     3721 ccacggcctg ctcgatgtct ggatgagcca cggcgacaag gtcaacgagt tgccgcccgg
     3781 attcaaggtg atcgcgtcca gcgcggcctg ccccatcgcc ggcatggcgg acgagggccg
     3841 caagctctat ggcgtgcagt tccatcccga ggtcacgcac acgttgcagg gaagggcgat
     3901 tcttgagcgc ttcgtgctgg aaacctgcgg gctcgccggc gactggagca tgccgaattt
     3961 catcgaggaa gcgatcggac gcatccgttc ggaggtcggc ccggaggaag tggtgctcgg
     4021 gctgtcggga ggcgtggact catcagtggc tgcggcgctc atccaccggg cgatcggcaa
     4081 gcagctcacc tgcgtgttcg tggatcacgg gctgctccgg ctcaatgagg cggagcaggt
     4141 gatgaacacc ttcgccggcc atctcggcgc cagtgtgatc cacgtcgacg cccgcgatca
     4201 attcatgaaa cagctcaagg gcgtggccga tcccgaagaa aagcgcagga tcatcggccg
     4261 cgagttcatc gaggtgtttc agcgcgaggc gttcaggctg ccccaggtga aatggctggc
     4321 tcagggcacc atctatcccg acgtgatcga gtcggcgggc gccaggaccc ggaaggcaca
     4381 cagcatcaaa tcgcaccaca atgtcggcgg gctgcccgac acgctgcacc tgaagctgct
     4441 cgagccgctg cgcgagctct tcaaggacga ggtgcgcgag ctggggaccg ccttgggcct
     4501 gccgcgcgac atcgttttcc ggcacccgtt cccgggaccg ggcctgggcg tgcgcatact
     4561 cggcgaggtc agggcggaat atgcggacct gctgcgacgg gccgacgcga tcttcataga
     4621 cgaattgcgc gagtcgggtt ggtacgacaa gacggcccag gcctttgcgg ttttcattcc
     4681 ggtgaagtcg gtgggcgtca tgggcgacgg gcgcacctac gagcatgtcg tcgcgctgcg
     4741 cgccgtgcag acccaggact tcatgaccgc gcaatgggcg gaactgcccc acgggctgct
     4801 gtccaaggtg gcgaaccgca tcatcaacga ggtgcggggc atcaaccgcg tagtctatga
     4861 catctcgagc aagccgcccg cgaccatcga atgggagtaa tgcaagcatt cagctttcag
     4921 ccgtcagcta tcagcctgaa cttggagatt ccgggtttag tgtcgctgac cgctgaatgc
     4981 tgaccgctga tggctccgcc atgagcgacg aaattttcat gcgcaaagcg ctgtcgctgg
     5041 cccgcgaggc ggccgcagct ggcgaggtgc cggtcggagc ggtcgtggtc aaggaggggg
     5101 ccgtggtcgg ctgcggctac aaccggccgg tgtcccggcg ggatcccacc gcgcacgccg
     5161 aggtgatcgc cttgcgggac gcggccgaga acatcggtaa ctaccggttg agcggatgcg
     5221 agctctacgt gacgctggag ccatgtgcga tgtgcgcggg cgcaatcatc catgcccgca
     5281 tcagccgggt cgtttacggg gccgctgacc ccaaggccgg tgcctgcggc agtgtcgtga
     5341 acttgttcga ggagggccgg ctcaatcacc atgccgtgat agccggcggc gtgatggccg
     5401 aggaggccgg caagctgctg caggacttct tctcggcgcg acgccagacg tcgagtcatg
     5461 cctagaataa acattaatat cagctcacaa gagcttgatg tttatgatga taatgacgcg
     5521 ctcctcaggc ggtacctgat ctcgagctcg aagaacgggg tgggcgagct gaacggcagc
     5581 tactgcaccc cccggggcaa acacatcatc cgcgccaaga tcggcgcagg ccagccggtc
     5641 aatgcggtat tcgtggagcg gcgcccaacg ggagaaatct acaacccgga actggctgag
     5701 cgcttccccg ggcgcgactg gatactcacc cgcattctct ggctgtcggg ctgcgagcgg
     5761 ggatacaacc gtctgggcaa ga
//
LOCUS       DBNGWZ010000518         4165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_574785, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000518 DBNGWZ010000000
VERSION     DBNGWZ010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_574785"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16CX_15875"
     CDS             <1..477
                     /locus_tag="AC16CX_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3014022.1"
                     /translation="AVRGPAAGIGFSLALACDLIIASESAYFLAAFKKVGIPPDGGAV
                     FFLTQYLGIARAKEIVYTARKLPAQEAREMGLVMKVVPDDRLEAEAQALARELADSAT
                     YALRLAKRMFHSMYVPTLEMLLEIENLAVCGARLTHDHNEGVVAFKEKRPPRFQGR"
     gene            complement(556..990)
                     /locus_tag="AC16CX_15880"
     CDS             complement(556..990)
                     /locus_tag="AC16CX_15880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3014023.1"
                     /translation="MSRYLIEVGLRGWTWPPERVAKAIKAPNTDVVVVEVKRHLVGFA
                     IMDFGDVTAHLSLLAVKPSHQRCGIGRQMMAWLEETALTAGITTVSLELRSNNFAARA
                     FYRMLGYKELSYIPGYYRGVETAVKMARDIRRSVPSALRRDA"
     gene            complement(1201..1776)
                     /locus_tag="AC16CX_15885"
     CDS             complement(1201..1776)
                     /locus_tag="AC16CX_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014024.1"
                     /translation="MIKRILVVTAALALPAAGLAQTLTGVTVEPAQIKAGESVKVTVN
                     FEVERGINCGLRLHFGDGNTVDYKINQEKDVPLIVPWKYGKAGDYRIMAEPKTVAPLL
                     KCNGKNQTTMLKVAAVSAAPEAPSKPSKTAKAAGPQCPDGWTLNKKSVNKKTGAFTCQ
                     AAPGTRPIKLACPGDLSYFENQKKGLMGCRP"
     gene            complement(2085..2654)
                     /gene="msrB"
                     /locus_tag="AC16CX_15890"
     CDS             complement(2085..2654)
                     /gene="msrB"
                     /locus_tag="AC16CX_15890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3014025.1"
                     /translation="MTRRGLLQGMLAAGLWNALTGRGDAAQPQAKGMKGVEELQKNWK
                     IHLAEGFKAPLPTDPLKLPKEEWRKRLSPLAYEVLREEGTERAGTSPLNNEKRDGVFV
                     CAGCDLPLFTSAMKYESGTGWPSFFTTIPGVFETTTDYKLFLPRTEYHCKRCGGHHGH
                     VFDDGPPPTGQRWCNNGVALKFIPKTGKA"
     gene            complement(2766..3758)
                     /locus_tag="AC16CX_15895"
     CDS             complement(2766..3758)
                     /locus_tag="AC16CX_15895"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014026.1"
                     /translation="MFTTPSGHWAQGAATLLVSLLLAAVAAPPARAQAYPARPIRLIV
                     PFPPGGSNDIVARMVGTQLTERLGKSVVVDNRGGAGGRIGYETAATSQPDGYTLLVIS
                     AAYAFTPALHKLNFDPVKSFVPVAMMGRGPNALAVYPGLGANSVKELVAMAKARPGQL
                     NYASAGFGTFQHLGSELFRIMAGISIVNVPFKGGGPATLAVVAGQAQISIGSLIQSLP
                     HVRTGRLKLLGVGGEKRVAILPDVPTISEAGVPGYQAYNWWGVVAPAGTPAAITRRLH
                     GEITGIVASQEVQKWFATEGAEAVNRTPEEFRKLIAAEIVKWGKVVKEAGIKAE"
     gene            3849..>4165
                     /locus_tag="AC16CX_15900"
     CDS             3849..>4165
                     /locus_tag="AC16CX_15900"
                     /inference="COORDINATES: protein motif:HMM:NF018949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR3014027.1"
                     /translation="MSSMLWIAFWLGLFAGAHLALSSLAVRARLVSMLGDKLFLGLYS
                     LIAFATFVPLVWIYFNNRHAGGLLWNVIALPGVRPFAMLLAALGIALIVAGVLQPSPV
                     LAG"
BASE COUNT          696 a         1369 c         1295 g          805 t
ORIGIN      
        1 gcggtgcgcg ggcccgcggc gggcatcggc ttcagcctgg ccctcgcctg cgacctcatc
       61 atcgcgtccg aatcggccta tttcctcgcc gcgttcaaga aggtcggcat cccgcccgac
      121 ggcggcgcgg tgtttttcct cacccagtac ctgggaatcg cgcgggccaa ggagatcgtc
      181 tacaccgcgc gcaagctgcc ggcgcaggaa gccagggaga tggggctggt gatgaaagtg
      241 gtacccgacg acaggctcga ggcggaagcg caggcgctcg cgcgggaact ggcggattcc
      301 gccacctacg cgctccggct cgcgaagcgc atgttccact ccatgtacgt gcccaccctc
      361 gagatgctgc tcgaaataga gaatctggcc gtgtgcggcg cgcgcctgac gcacgaccac
      421 aacgagggag tggtcgcgtt caaggaaaaa cgcccgccaa ggttccaggg gcgctgaaaa
      481 gcgtgataag caacaggagt caaagctggc gctgccagcc gttcttgtca cttatcccgt
      541 atcacgcacc acgcatcacg catcccggcg cagcgcgctc ggcacgctac gccggatgtc
      601 gcgcgccatc ttcaccgcgg tttccactcc gcgatagtaa ccgggaatgt aggacagctc
      661 cttgtaaccc agcatgcggt agaaggcgcg cgcggcgaag ttgttggagc gcagctcgag
      721 gctcacggtg gtgatgccgg cggtcagcgc cgtctcctcc agccacgcca tcatctgcct
      781 gccgatgccg cagcgctggt gggagggctt caccgcgagc agcgagaggt gcgcggtgac
      841 gtcgccgaag tccatgatcg cgaacccgac caggtggcgc ttcacctcca ccaccaccac
      901 gtcggtattg ggcgccttga tcgccttcgc cacccgctcc ggcggccagg tccagccgcg
      961 cagcccgact tcgatcaggt agcgcgacat caccgcgatt tcataggcgt ccgcggggca
     1021 tgcgagtcgg aaggcgggtg tggacataag cgtaaatata cccaatcctt gattgcgtga
     1081 cgagtgagag ctgatgggtg atgagtgata ggtgatagga aaaaacaaag gcggcgcaat
     1141 gcgccgcccc gtcggatacc acctatcact tatcactcgt cacttatcac gcctttggcc
     1201 tcagggcctg cagcccatca aacctttctt ctggttctcg aagtagctca ggtcccccgg
     1261 acaggcaagc ttgatcggcc gggtgccggg cgccgcctgg caggtgaatg cgccggtctt
     1321 cttgttgacg gacttcttgt tcagcgtcca gccgtccggg cattgcggac cggccgcttt
     1381 ggcggttttt gacggtttcg agggagcctc cggtgccgcc gacacagcgg cgaccttgag
     1441 catggtcgtc tggttcttcc cgttgcactt gaggagcggc gccaccgtct tcggctcggc
     1501 catgatccgg taatcgcccg ccttgccgta cttccacggc acgatcaggg gcacgtcctt
     1561 ttcctgattg atcttgtagt caaccgtgtt gccgtcgcca aaatgcagcc gcaagccgca
     1621 gttgatgccg cgctccactt cgaagttcac cgtgaccttg actgactcac ccgccttgat
     1681 ttgcgcgggc tcgaccgtga caccggtcag ggtctgggcc agcccagccg ccggcagagc
     1741 gagtgcggcg gtaacaacca gtatgcgctt gatcatattt cctccctgtg cggttgcgta
     1801 gaagccggag gtaattttgc acttgcagat gccgttcttc aaggacaaaa aagcccggcg
     1861 cgaggccggg cttttttcac actcgtcgct tatcacccgt cagtggcctc agtcatgcca
     1921 ccgtgaattt caacgtcaac tattcggccg ctctgcgcgc cgccgggagt cacatcccga
     1981 acaagttcgg ggcgcgtcgt agggcggaag atgggacccg gtgataatac ggggacctga
     2041 ccccggagac gcgcacggga tcggtatcgt gacgacgcgc cccatcacgc ttttccggtc
     2101 ttcgggatga atttgagcgc cacgccgtta ttgcaccagc gctggccggt cggcggcggg
     2161 ccgtcgtcga agacgtggcc gtggtggccg ccgcagcgct tgcagtggta ctcggtgcgc
     2221 ggcaggaaca gcttgtagtc ggtcgtggtc tcgaatacgc cgggtatggt ggtgaagaag
     2281 ctcggccagc cggtaccgct ttcgtacttc atcgcggaag tgaagagcgg caggtcgcag
     2341 ccggcgcaga cgaagacgcc gtcacgcttc tcgttgttga gcgggctggt ccccgcgcgc
     2401 tcggtgcctt cctcgcgcaa cacctcgtaa gccagcggcg acaggcgctt gcgccactcc
     2461 tccttcggga gcttgagcgg atcggtgggc agcggggcct tgaagccctc ggcgagatga
     2521 atcttccagt tcttttgcag ctcctcgacg cccttcatgc cctttgcctg tggctgggcg
     2581 gcgtcaccgc gcccggtcag cgcgttccac agccccgccg ccagcatgcc ctgcaacaaa
     2641 cctctgcgtg tcatatcgtc tcctcagatc attcaccgca cttgcggatg cccccatata
     2701 cggatcatca gccgccgcgg atgaggctga tgatgaagcg aaaccggatg atattcatcc
     2761 tcatgctact cggccttgat gccggcttcc ttcacgacct tcccccattt gacgatctcg
     2821 gccgcgatga gcttgcgaaa ttcctcgggg gttcggttga cagcctccgc gccttccgtg
     2881 gcgaaccatt tctgaacttc ctgcgaagcg acgatgcccg tgatctcgcc gtggaggcgc
     2941 cttgtgattg cggccggcgt tccggcgggg gcgacgacgc cccaccagtt gtacgcttgg
     3001 taacccggca cgccagcctc ggaaattgtg ggcacatcgg gtagaatcgc cacgcgcttt
     3061 tcgccgccga ctcccagcaa cttcaaccgt cccgtacgca cgtggggcag cgactggatc
     3121 agcgagccga tcgaaatctg ggcctggccc gccacgaccg cgagcgtggc cggccccccg
     3181 cccttgaaag gcacgttcac gatgctgatc cccgccatga tcctgaaaag ctcgctcccg
     3241 aggtgctgga aggtgccgaa tcccgccgag gcgtaattga gctgcccggg cctcgctttc
     3301 gccatagcga caagttcctt cacggaattg gcgccgagac caggatatac cgccagcgca
     3361 ttcggcccgc gtcccatcat ggcgaccggg acgaatgact tgaccggatc gaaattgagc
     3421 ttgtggagcg cgggagtgaa ggcgtatgcg gcggaaatga ccagcagcgt gtaaccgtcc
     3481 ggctgcgacg tggcggcggt ttcatacccg atgcgaccgc ccgctccgcc gcggttgtcc
     3541 accaccaccg attttccgag ccgctccgtg agctgcgtgc ccaccatgcg cgccacgata
     3601 tcgttgctgc cgcccggcgg aaacgggacg atcagccgga tcggccgcgc ggggtaggct
     3661 tgcgcccgcg cgggcggcgc tgcgaccgcg gcgagcagta acgagaccag cagcgtcgcg
     3721 gcgccctgtg cccaatgtcc cgacggtgtg gtgaacatat cgaatcctcc cccggtgtcg
     3781 ggtgtgcctt cgggcagatt ctagcagacg cgcggcagcg ccccggcgcg ctacaatccc
     3841 cgttaagcat gagctcgatg ctctggatcg ctttctggct cggcctgttc gccggcgcgc
     3901 acctcgcgct gtcctccctc gcggtgcgcg cgcgcctcgt ttccatgctc ggcgacaagc
     3961 tcttcctcgg cctctactcg cttatcgcct tcgccacctt cgttccgttg gtgtggatct
     4021 atttcaacaa ccggcatgcc ggtgggttgt tatggaacgt catcgcgctg ccgggtgtgc
     4081 gcccgttcgc catgttgctc gccgcgctcg gaatcgcgct gattgtcgcg ggcgtgctcc
     4141 agccgagccc ggtgctcgcc ggatt
//
LOCUS       DBNGWZ010000519         2692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_823683, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000519 DBNGWZ010000000
VERSION     DBNGWZ010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_823683"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16CX_15905"
     CDS             complement(<1..611)
                     /locus_tag="AC16CX_15905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3014028.1"
                     /translation="MKTRNIKVQLRDGVMGAHLAIPERAPAGAIIAIMEIWGVNDTMR
                     RHAAEFAEVGFVCLVPDLFWRQEPGVELSDSNPEHVKKAFDLYYDFDYDLAVRDMEDT
                     WRYLAGIPECNGKVGAVGYCLGGKLCYLMCCRTDIDCAVAYYGTYIEHHIREVGNLHR
                     PFVLHMAMRDRWVQAEVNELLERRLSPNPLVTIHKYPGADHAFA"
     gene            complement(791..1765)
                     /locus_tag="AC16CX_15910"
     CDS             complement(791..1765)
                     /locus_tag="AC16CX_15910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014029.1"
                     /translation="MRIHCRWISTFVLTVLALGAAPAAELPYPTRPIRMVLPASPGGP
                     VDVIARTVGAGLAEALGQQIVMDNRAGAGGIIGAEIVARATPDGYTLMFAHSGPLAIE
                     AAIHSKLPYHPLKDFAPVSLVAASPYALIVNPATPAKSVKELVALAKSRPGKFHYASG
                     GVGTGIHMAGELLNLAAGLKMVHVPYKGAGPSMAALMSGEVDMMFNGVSSALPHVKAG
                     RLRALAISTAQRSPLLPDLPTVAESGLRYETSGWYGLVAPARTPKTVTARLQSHLLKA
                     LNTPEMKERLASQGIDGIASTPERLTQHLREEIDKWTAVVKAAGLKAD"
     gene            1949..>2692
                     /locus_tag="AC16CX_15915"
     CDS             1949..>2692
                     /locus_tag="AC16CX_15915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3014030.1"
                     /translation="MKRFRYLWRALNYRLRVSPREIEFLSRTVRAGDTVIDVGCHKGG
                     FLYWLRRYVTASGKVYGFEPQPQLAQYLKEIAAMQRWDNVIVEAAGVSSTNGSMELFI
                     PGHHGEASPGATFSSGGLNEPHHSIRVPVVTLDAYFQQRGDPRIACIKCDCEGHEIEA
                     FRGAEGLLRRDRPALLFECEQRHLPGASPDVVFDYLRGLGYRGFFFSPAGLTPVEQFR
                     PEIHQPVRPGRYWDAKDYYNNFAFLPEASK"
BASE COUNT          498 a          845 c          826 g          523 t
ORIGIN      
        1 gcgaacgcgt ggtccgcgcc cggatatttg tggatcgtga ccaaagggtt gggcgagagc
       61 ctgcgctcca gcagctcgtt cacttccgcc tgcacccagc ggtcgcgcat cgccatgtgc
      121 agcacgaacg gccggtgaag attgccgacc tcgcggatgt ggtgctcgat gtaggtgccg
      181 taataggcga ccgcgcaatc gatgtcggtg cggcagcaca tcaggtagca gagcttgccg
      241 cccaggcagt agccgaccgc cccgactttt ccgttgcatt ccgggatgcc cgcgaggtat
      301 ctccaggtgt cctccatgtc gcgcacggcg aggtcgtaat cgaagtcgta atagaggtcg
      361 aatgcctttt tcacgtgttc cgggttggaa tcggagagct ccacgccggg ctcctgccgc
      421 cagaagagat cagggacgag gcagacgaaa ccgacctccg cgaactcggc cgcatggcgc
      481 cgcatcgtgt cgttgacgcc ccagatttcc atgatcgcga tgatcgcgcc ggccggcgcg
      541 cgctccggga tggcgaggtg ggcgcccatc acgccatcac gcagctggac cttgatgttt
      601 cgcgtcttca tgctcaagaa cggaatttag ccgccgatga acgccgacaa agtcaaggac
      661 aaaaaatgaa cgggattaac atgattcaca ggattggatc aaaaaccaaa ccgatcgaca
      721 tcaggttttt gttgtttgaa tctcgttaat cgtgtaaatc ctgtctattt cttgaatttc
      781 accggcggtt tcaatccgcc ttcaggccgg ccgccttgac cacggcagtc catttatcga
      841 tctcctcgcg caggtgctgg gtaagccgct ccggggtgct cgcgatgcca tcaatgccct
      901 ggctcgcgag gcgttccttc atctcgggcg tgttcagcgc cttcaggaga tgcgactgca
      961 gcctggccgt gacggttttc ggcgtgcgcg cgggcgctac cagaccgtac catccggagg
     1021 tctcatagcg caggccgctc tcggccaccg tcggcagatc gggcagcagc ggcgagcgct
     1081 gcgcggtgct gatcgcaagc gcgcgcaacc gtcccgcctt cacgtgcggc agcgccgaag
     1141 acacgccgtt gaacatcatg tccacctctc cgctcatcag cgccgccatg ctcgggcccg
     1201 cgcccttata cggcacgtgg accattttga ggccggcggc gaggttgagc agctccccgg
     1261 ccatgtggat cccggtcccg acgccgcccg aggcgtaatg gaatttcccg ggccgcgatt
     1321 tcgccagcgc aaccagttct ttcacggatt tcgcgggtgt cgcggggttc acgatcaacg
     1381 cgtacggcga agcggccacc agcgacactg gcgcgaagtc cttcagcgga tgatacggga
     1441 gcttcgagtg tattgccgct tcgatcgcga gcggcccgga gtgtgcgaac atcagcgtgt
     1501 agccatccgg ggtggcccgc gccacgatct cggcgccgat gatgccgccg gcgcctgcgc
     1561 gattgtccat cacgatttgc tgccccaatg cttccgcaag acccgctccc accgtgcggg
     1621 cgatgacgtc gaccggacca cccggtgaag ccggcaggac catcctgatg ggccgggtcg
     1681 gatacggcaa ttcagcagct ggtgccgcgc ccaacgccaa gaccgtcagg acgaacgtac
     1741 ttatccagcg gcagtggatt cgcatgggcc ccctccttgt tgtagttgac tgcgaatgcc
     1801 ccggtattct atctcacgga tcgaacgata aatgcgtgac gaagtgatga gcgcttgatc
     1861 aataaaatcc gccacgcgtc atcatgtcaa gcagtatcat gtcacgcatc atcgtgtcac
     1921 gcagtatcac gtcacgcgcc tacaattgat gaaacggttt cgctatctgt ggcgggcgct
     1981 gaactaccgg ctgcgggtga gcccgcgcga gatcgaattc ctctcgcgga cggtccgtgc
     2041 gggtgacacc gtgattgacg tcggctgcca caagggcggt ttcctgtact ggctccggcg
     2101 ctacgtcacg gcctcaggaa aggtctacgg attcgaaccc cagccgcagc tcgcgcagta
     2161 cctgaaggaa atcgcggcga tgcagcgctg ggacaacgtg atcgtggagg ccgccggagt
     2221 ttcgtccacg aatggctcaa tggagctgtt catcccaggg catcatggag aagcgtcccc
     2281 gggagcgact ttttcctccg gcggcctgaa tgaaccgcat catagtattc gcgtgccggt
     2341 cgtcactctg gatgcgtact tccagcagcg aggcgatccc cggatcgcgt gcatcaagtg
     2401 cgattgcgaa ggtcacgaga tcgaagcgtt caggggcgcc gaaggtctat tgcggcgcga
     2461 ccggccggcg ctgctgttcg agtgcgagca gcgccacctc cccggagcct cgccggatgt
     2521 cgtgttcgac tatctgcggg ggctcggcta ccgaggcttc ttcttcagcc ccgccgggtt
     2581 gacgcccgtc gagcagttcc ggccagaaat ccaccagccg gtccgccccg gccgttattg
     2641 ggacgcgaag gactactaca acaacttcgc cttcctgccg gaggcttcaa ag
//
LOCUS       DBNGWZ010000520         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2587267, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000520 DBNGWZ010000000
VERSION     DBNGWZ010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2587267"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16CX_15920"
     CDS             complement(<1..230)
                     /locus_tag="AC16CX_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG4315 family predicted lipoprotein"
                     /protein_id="HIR3014031.1"
                     /translation="MFRTEAAIAILGSMLAVTGCAGSAPAVYSLGILVNPAGMTLYTF
                     DKDPAGKSVCNDKCAANWPPLKASAGARPGGE"
     gene            complement(308..>1927)
                     /locus_tag="AC16CX_15925"
     CDS             complement(308..>1927)
                     /locus_tag="AC16CX_15925"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR3014032.1"
                     /translation="LRLQGRLAEAIVHYRHALDADPKRAMAHSELGAAHRQAGDLDSA
                     VACFRKANEVDPGHANTYYELAETFKLQGRLDSAIDSYERALALKPGHSLALGGLTYL
                     RQHMCDWNGIDALWARLRGEAIGKAESGVSPFSVLYMPFSAEEQLACAREWARQTLDH
                     LAAARLGLGFGESPRERHSRTRIGYLSWDFHQHATSYLVAELFELHDRGRFEVFAYSY
                     GPDDGSPVRARIRRACEHFADVSGESFVDTAQRIRRDGIDILVDLKGYTLGSRPQILA
                     LRPAPIQVNWLGYPGSMGAKCVDYILADPFIIPEGAERRYSEKVVRLPDCYQVNDRKR
                     EISERVPDRKESGLPASGVILCCFNLSAKILPDVFAAWMRILKTVPDSVLWLLETNHW
                     AAANLRHAAANHGVAPDRLVFAPHKPLPEHLARYRLADLSIDTFPYTAHTTASDALWA
                     GCPLVTRVGETFASRVAGSILRAAGLPELVTQNADDYERLVIELATSPERLRSLKTRL
                     NENRHSCPLFDTPRFVKNLENAYETMLNVSG"
BASE COUNT          334 a          645 c          613 g          335 t
ORIGIN      
        1 aattcgccgc cgggcctcgc ccccgcgctg gccttcagcg gcggccaatt ggcggcgcac
       61 ttgtcgttgc acacgctctt gcccgcgggg tccttgtcga aggtatacaa cgtcatgccc
      121 gccggattga ccaggatacc gagcgagtag acggcggggg cggaacccgc gcagcccgtc
      181 acggccagca tgctccccag tatcgcgatc gccgcctcag tcctgaacaa aggtccctcc
      241 gttgatgagt cgattcagcg gtgcaaacat ccgcgccgcc cggtatattc ccgcgcccga
      301 ccggctgcta tccggaaacg ttgagcatgg tttcgtaagc gttctccagg ttcttgacga
      361 accgcggcgt atcgaagagc ggacaggaat ggcggttctc attgaggcgc gtcttcaagc
      421 tccgcagtcg ttccggggaa gtggccaatt caatcaccag gcgctcgtag tcgtccgcgt
      481 tttgcgtcac cagttccggc agaccggcgg cacgcaggat gctgccggcg acgcgtgagg
      541 cgaacgtctc acccacgcgt gtcaccagcg ggcaccccgc ccacagcgca tcgctcgccg
      601 tggtgtgcgc cgtgtaggga aacgtatcga tcgaaaggtc ggcgagtcgg tagcgggcga
      661 ggtgctcggg caacggcttg tgcggcgcaa agacgagtcg gtcgggcgcg acaccatgat
      721 tcgccgccgc gtgcctcagg ttcgccgcgg cccagtgatt ggtttcgagc agccatagca
      781 cgctgtcggg caccgttttc aggatccgca tccaggccgc aaaaacatcc ggcaggatct
      841 tcgcgctcag gttgaagcag caaaggatga cgccggatgc gggcagtccg ctttcctttc
      901 ggtcaggcac acgctcgctg atctcgcgct tgcggtcgtt cacctggtag caatcgggaa
      961 gccgcaccac cttttcggaa tagcggcgct cggccccttc cggaatgatg aaggggtcgg
     1021 ccagtatgta atccacgcac ttggctccca tgctgcccgg gtagcccagc cagttcacct
     1081 gtatcggcgc cggccgcagc gcgaggatct gggggcgcga gcccagggtg tagcccttga
     1141 gatcgaccag gatgtcgatc ccgtcacgcc ggatccgttg cgcggtatcc acgaaggact
     1201 cgccggaaac gtcggcaaaa tgctcgcagg ccctgcggat gcgcgcccgg acggggctgc
     1261 cgtcatcggg accataagaa taggcaaaga cctcgaaccg gccgcgatcg tgcaactcga
     1321 agagttcggc caccaggtac gacgtcgcgt gctgatggaa gtcccacgac agatagccga
     1381 tccgcgttct cgaatggcgc tcccgcggcg actcgccgaa tccgaggccc aggcgcgctg
     1441 cggccaggtg atcgagcgtc tggcgcgccc actcccgggc gcatgccagc tgctcctccg
     1501 ccgaaaacgg catgtacagt acgctgaacg gcgagacccc cgactccgcc ttgcctatcg
     1561 cctcgccgcg caaccgcgcc cacagggcgt cgatgccgtt ccaatcgcac atgtgctggc
     1621 gcaggtaggt cagcccgccc agcgccaggg agtgtcccgg tttcagggcc agcgcgcgct
     1681 cgtacgaatc aatcgcactg tcgagccgtc cctgcagctt gaacgtttct gcgagttcat
     1741 aataggtgtt ggcatggccc gggtccactt cgttcgcctt gcggaagcac gcaaccgcgg
     1801 aatcgagatc gccggcctgc cggtgggcgg cccccagctc gctgtgggcc atcgcccgct
     1861 tcgggtcggc atcgagcgcg tgccgatagt gaacgatcgc ctccgccagc cgtccttgca
     1921 gcctcag
//
LOCUS       DBNGWZ010000521         5149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2840680, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000521 DBNGWZ010000000
VERSION     DBNGWZ010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2840680"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="AC16CX_15930"
     CDS             <1..349
                     /locus_tag="AC16CX_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014033.1"
                     /translation="INGCEFCVDLNSFLGLGRGIAEDKLRALPLFEDSTLFSERDKSA
                     LAYAEAATRSDRRVGEELILRLRKHFDDQAIVELAALIAYQNMSSKFNAALGVPAHGF
                     CAVAGRSPSRKGA"
     gene            354..872
                     /locus_tag="AC16CX_15935"
     CDS             354..872
                     /locus_tag="AC16CX_15935"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3014034.1"
                     /translation="MLTHRYECSGIVTAPVERVFAHIDDHARLSSHMSESSWMMGGSR
                     MEIELDDGRGQKIGSRIRLAGRVFGVELSVEEVVTERIPPRRKVWETTGSPKLLVIDH
                     YRMGFELSPHGADSMLRVFIEYALPARAPARWLGRLFGGYYARWCTQRIVEDAVKHFA
                     PFASARVRSSNA"
     gene            891..1172
                     /locus_tag="AC16CX_15940"
     CDS             891..1172
                     /locus_tag="AC16CX_15940"
                     /inference="COORDINATES: protein motif:HMM:NF039498.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5676 family membrane protein"
                     /protein_id="HIR3014035.1"
                     /translation="MTRLNPWIFGVVVSITVVVIYAICTLLWFAFPEPSIKFINALFH
                     GMDFRKIYASAAFSFGDFLYVLVVFTVWGYILGVVYATVRNLLLAGGKA"
     gene            complement(1169..1615)
                     /locus_tag="AC16CX_15945"
     CDS             complement(1169..1615)
                     /locus_tag="AC16CX_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3014036.1"
                     /translation="MVDLMDFTIGDVAEAAGVGVETVRYYERRGLVPQPNRRLGSYRR
                     YGNGHVDRIRFIRRAQALGFSLEEIASLLELEDGTDRRSIRRIAAARLDEMRRRMADL
                     KRIERVLAHLLHECEAHSKAPRCPIISAIATARDEGAEPDRRDPRH"
     gene            1693..4440
                     /locus_tag="AC16CX_15950"
     CDS             1693..4440
                     /locus_tag="AC16CX_15950"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3014037.1"
                     /translation="MNDTRHDHDRHGHACCGHGAGADVRKEPTKHSDGKGAHACCGGA
                     AHGHQQGAAAPVTDPVCGMQVDPKSAAGVHEHEGVKYYFCSKHCLAAFQADPAKYLAK
                     RAPSAQPEAAPARATDPVCGMKVDPRSAAGVHEHDGVKYYFCSKHCLAAFKADPAKYS
                     ARIVPPPPAAGSKDAMYICPMCPGVRQQGPGSCPKCGMALEPEAPVAAATRTEYVCPM
                     HPEIVRSEPGNCPICGMALEPRTVTLEEEANPELADMTLRFWVSTVLAAPLFVAATAA
                     DLWPELAARIISPRVLQWVQFLMATPAVLWGGWPIFVRGWQSIVNRSLNMFTLIALGV
                     GVAWTYSTVALLLPGIFPATLLTAEGLVPVYFEAAAVITALVLLGQVLELRARSRTSA
                     AIKMLLGLAPKTARIVRADGREEDIPLEQVKPGDVLRVRPGEKIPVDGVVADGASSLD
                     ESMVTGEPIPVEKHRGDRVIGATVNGTGSLVMRAEKVGADTLLAHIVRMVAEAQRSRA
                     PIQRLVDIVTAYFVQVVVAVAAVTWVVWFFWGPEPALAYAVINAVAVLIIACPCALGL
                     ATPMSIMVGTGRGALAGVLIKNAEALEIMGKVDTLVTDKTGTLTLGKPKLTTVAVAPG
                     FAEDEILRLGASLERASEHPLAAAIVGGAEAKGLTLAPVERFQSVTGKGVTGDVGGRA
                     VAIGNRKLLETLGVDPGELDKRAEALRAEGQTVMFVVADGKPAGLIGVADPVKETTPE
                     AIRALHEEGVKIVMLTGDNRVTAEAVARKLGIDRVMAEVLPEQKTEIVKQLQAERRVV
                     AMAGDGINDAPALAQAQVGIAMGTGTDVAMESAGVTLVKGDLRGIVRARRLSRATMRN
                     IRQNLFFAFIYNVLGIPVAAGVLYPVFGLLLSPIIAAAAMSFSSVSVVTNALRLNRVR
                     L"
     gene            4510..4707
                     /locus_tag="AC16CX_15955"
     CDS             4510..4707
                     /locus_tag="AC16CX_15955"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR3014038.1"
                     /translation="MAIDPVCKMNVDPASAAAKAEYAGQTYYFCCAACQKTFAAQPEK
                     YVSNTARGSHSPGGTHHHGHS"
     gene            4750..>5149
                     /locus_tag="AC16CX_15960"
     CDS             4750..>5149
                     /locus_tag="AC16CX_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3014039.1"
                     /translation="MNDANLVREAASTTVEAQAPAPRLRRNLWPLAIFAVIAVFLGVG
                     LTLNPREIPSPLIGKPVPEFRLEPVKGRTLGLASSDLRGEVSVVNVFASWCVECRAEH
                     PLWMQIKAEGSVPLHGLNYKDRPDDAARWLG"
BASE COUNT          892 a         1647 c         1738 g          872 t
ORIGIN      
        1 gatcaacggc tgtgaattct gcgtggatct gaactcgttc ctcggtctcg gtcgcggtat
       61 cgcggaggac aagctacgcg ctctgccgct gttcgaggat tcgacattgt tcagcgaacg
      121 cgataaatcg gcactcgcct atgccgaggc agccacgcgc agcgaccgcc gtgttggcga
      181 ggaactcatc ctgcgtttgc gcaagcattt tgacgatcaa gcgattgtcg agcttgctgc
      241 gctgatcgcc taccagaaca tgtccagcaa gttcaatgcg gcgctcggcg tgccagcgca
      301 tggcttttgc gccgtcgctg gacgctcccc ctcgcgaaaa ggagcgtgaa tccatgctga
      361 cacatcgtta cgaatgcagc gggattgtca cggcccccgt ggaacgggtt ttcgcgcaca
      421 ttgacgatca cgcgcggctg tcctcccaca tgagcgagtc ctcgtggatg atgggaggga
      481 gccggatgga gattgaactc gacgacggcc ggggacaaaa gatcggatcg cgaattcgcc
      541 tggcgggacg agtctttggc gtcgagcttt cggtcgaaga ggtcgtgacc gaacgcattc
      601 ctccccgtcg caaggtctgg gaaacgacgg gctcgcccaa gcttctggta atcgatcatt
      661 accgaatggg tttcgagcta tcgccccacg gggcggattc gatgctccgc gtctttatcg
      721 agtatgcgct gccggcaaga gcgcctgcgc gctggctggg acgcttgttt ggcgggtact
      781 acgccagatg gtgcacgcag cggatcgtgg aagacgcagt gaagcatttc gcgccgttcg
      841 cgtccgcgcg cgtgcgcagc tccaatgcct gacactcaac ggaggccatc atgacaagac
      901 tcaatccctg gattttcggc gtggtcgttt caatcacggt cgtggtcatc tacgccatct
      961 gcacgctgct ctggttcgcg ttccccgagc cctccatcaa gttcatcaat gcgctgtttc
     1021 acggcatgga tttcaggaag atctacgcaa gcgccgcgtt ttcgttcggc gacttcctgt
     1081 atgtgctcgt cgtgttcacc gtctggggct acatcctcgg cgtcgtctat gcgacggttc
     1141 gcaatctgct gcttgcggga ggtaaggcct aatggcgcgg gtcccgccga tcgggttcgg
     1201 cgccctcatc gcgcgccgtc gcgatcgcgc tgatgatcgg gcaacgcggc gccttgctgt
     1261 gggcttcgca ttcatgaagc aggtgcgcga gcacgcgctc gatgcgcttg aggtcggcca
     1321 ttcggcgccg catctcgtcg agccgcgcgg cggcgatgcg ccggatgctg cggcgatccg
     1381 ttccgtcctc gagctcgagc agcgaggcga tctcctccag gctgaacccg agcgcctgcg
     1441 cacgcctgat gaagcggatg cgatccacgt gaccgtttcc gtagcggcga taggacccca
     1501 acctgcggtt tggctgcggc accagccccc gccgctcgta gtagcgcacg gtttcgaccc
     1561 cgacacccgc ggcctcggcg acgtccccga tcgtgaaatc catgagatcc accataaaca
     1621 cccccttgac tccgtaccga agtacggact ttacactcac ggcgtaactg actcaatagc
     1681 agggagcaaa gcatgaacga cacacgacat gatcacgacc gccacgggca cgcctgctgc
     1741 ggccacggtg ccggggctga tgtacggaag gagcccacga agcacagcga tggcaagggc
     1801 gcccatgcct gctgcggtgg cgcagcccac ggccaccagc agggggcagc cgcaccggtg
     1861 acggaccctg tctgcggcat gcaggtggac ccaaagtccg ccgcgggcgt gcacgagcac
     1921 gaaggcgtca agtactactt ttgcagcaag cattgccttg ccgcgttcca agccgatcct
     1981 gcgaagtacc tggctaagcg cgcgccttcc gcgcagcccg aggccgcgcc cgcgagagca
     2041 accgacccgg tctgcggcat gaaagtggac cccaggtccg ccgccggtgt gcacgaacac
     2101 gacggcgtca agtactactt ctgcagcaag cactgccttg ccgcattcaa agccgatccc
     2161 gccaagtact cggccaggat cgttccacct ccgcccgccg ccggctcgaa ggatgcgatg
     2221 tacatctgcc cgatgtgccc cggcgtacgg cagcaaggcc ccgggagctg cccgaaatgc
     2281 ggcatggcgc tggagccgga agcgccggtc gcggccgcga cgcgcaccga gtacgtgtgc
     2341 ccgatgcatc ccgagatcgt gcgcagcgag ccgggcaact gcccgatctg cggcatggcg
     2401 ctcgaaccaa ggaccgtgac gctggaggaa gaggcgaacc ccgagctcgc ggacatgacg
     2461 ctgcgcttct gggtgagcac ggtgctcgcg gccccgctct tcgtcgcggc gaccgcggcg
     2521 gacctgtggc cggaactagc cgcgcggatc atctcgccgc gcgtgctgca gtgggtgcag
     2581 ttcttgatgg cgacaccagc ggtgctgtgg ggcggctggc cgattttcgt gcggggctgg
     2641 cagtcgatcg tgaatcgcag cctgaacatg ttcacgctca ttgcgctggg cgtgggcgtt
     2701 gcatggacct acagcaccgt ggcattgctg ctacccggga tctttcccgc cacattgctg
     2761 accgcagagg ggctggtgcc cgtctacttc gaggccgccg ccgtgatcac cgccctggtg
     2821 ctgctggggc aggtgctgga actgcgcgcc cgcagccgca ccagcgcggc gatcaagatg
     2881 ctgctcgggc tcgcgcccaa gacggcgcgt atcgtgcgcg cggatggccg cgaagaagac
     2941 atcccgctag aacaggtgaa gcccggcgac gtcctgcgcg tgcgcccggg cgaaaagatc
     3001 cccgtggacg gcgtcgtggc cgacggcgcc agttcgttgg acgaatcgat ggtgaccggc
     3061 gagccgatcc cggttgagaa gcaccggggc gaccgcgtca tcggggcgac ggtgaacggg
     3121 accgggagcc tcgtcatgcg cgccgaaaag gtgggggccg acacgctgct cgcgcacatc
     3181 gtgcgcatgg tcgcggaggc ccagcgcagc cgcgccccga tccagcggct ggtcgatatt
     3241 gtgaccgctt atttcgtgca ggtggtcgtg gccgtcgccg ccgtcacgtg ggtcgtatgg
     3301 ttcttctggg gccccgagcc ggctctggcg tacgcggtga tcaacgcagt cgcggtgctc
     3361 atcatcgcgt gcccgtgcgc gctggggctg gccacgccga tgtcgatcat ggtcggcacc
     3421 gggcgcggcg cgctggccgg tgtgctgatc aagaacgccg aagcgctgga gatcatgggc
     3481 aaggtggaca cgctcgtcac ggacaagacc ggcacgctga ccctgggcaa gccgaagctc
     3541 acgacggtgg cagtcgcgcc cggtttcgcg gaagacgaaa tcctgcggct cggggcgagc
     3601 ctcgagcgcg cgagcgagca tcccctcgcc gcggcgatcg tcggcggtgc ggaagccaaa
     3661 gggctgactc tcgcgccggt cgagcgattc cagtccgtga ccggcaaggg cgtgacgggc
     3721 gacgtcggcg gacgggcggt ggcgatcgga aatcgcaagc tgctcgaaac gctcggcgtc
     3781 gaccccggcg agctggacaa gcgcgccgag gcgctgcgtg ccgagggcca gaccgtgatg
     3841 ttcgttgtcg cggacgggaa gcccgccggg ctgatcgggg tggccgaccc ggtcaaggag
     3901 accacgccgg aggcgatccg ggcgttgcac gaggagggcg tgaagatcgt gatgctcacg
     3961 ggcgacaacc gtgtgaccgc ggaagcggtc gcgcgcaagc tcggtatcga ccgcgtcatg
     4021 gccgaggtgc tgcccgagca gaagaccgag atcgtcaaac agctccaggc cgaacgccga
     4081 gtggtggcga tggcggggga cgggatcaac gatgcgccgg cgctcgcaca ggcccaggtg
     4141 ggcatcgcga tggggaccgg cacggatgtc gcgatggaaa gcgcgggcgt gacgctcgtc
     4201 aagggtgacc tgcgcggtat cgtgcgcgcg cgccgcctga gccgcgccac gatgcgcaac
     4261 atccgccaga acctgttctt cgcgttcatc tacaacgtgc tcggaatacc ggtggcggcc
     4321 ggggtgctgt atcccgtctt cgggctgctg ctctccccca tcatcgccgc cgctgcgatg
     4381 agcttcagct cggtgtcggt ggtgacgaac gcattgcggc tgaaccgggt aaggctatga
     4441 cgcgctcgtg caaaggccca ctgaacctgt agcggacgga aacgtaagtc aacctgtcgg
     4501 aggcaaaaca tggcaatcga tcctgtgtgc aagatgaacg tggacccggc cagcgcggcg
     4561 gcaaaggcgg agtacgcggg acaaacctat tacttctgtt gcgcggcgtg ccaaaagacg
     4621 ttcgccgcgc aaccggagaa gtatgtgagc aacacagcgc gcggcagcca ctcgcccggc
     4681 ggaacgcacc accacggcca tagctgagcg gcacagcctg ggccgcatgt cccgtccgtg
     4741 tcctgcagca tgaacgacgc aaatctggtc cgcgaggccg cctccacgac agtggaagcg
     4801 caagcgcccg cgccgcgcct gcggcgcaat ctttggccgc tcgcgatctt cgccgtcatc
     4861 gccgtgttcc tgggcgtcgg cctgaccctc aatccgcgcg aaatcccctc accgctcatc
     4921 ggcaagccgg tgcctgaatt ccggctcgag ccggtcaagg gccgcacgct gggtctcgcc
     4981 tcgagcgact tgcgtggcga agtctcggtt gtcaacgtat ttgcctcgtg gtgcgtggag
     5041 tgtcgcgcgg agcatccgct gtggatgcag atcaaggcgg agggcagcgt cccgctgcac
     5101 ggtctcaatt acaaggaccg gccggatgac gccgcacggt ggcttggcg
//
LOCUS       DBNGWZ010000522         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2841209, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000522 DBNGWZ010000000
VERSION     DBNGWZ010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2841209"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="AC16CX_15965"
     CDS             <1..813
                     /locus_tag="AC16CX_15965"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014040.1"
                     /translation="TGERLGQQIVIDNRAGASGTIGVEMAARATPNGYTWGLGTTSTH
                     ALAAILNPKLGYDPARDFAPVVVLGDSAYFLTTNPTLPATNIREFIAHARANPGKLHY
                     ASVGNFSLGHLSGELLKKAAGIDMVHVPYKGSTLALIDLLAGRIQLNISTIAASLPQV
                     KAGKLRALAVTSRERVSALPQTPTVAESGFPGYQASLWMGVFVPARTPGAVVAALNRE
                     LGSTLRSAELREALAEQAVEPGAGAPGALAKLLREDTARWRKVIQDAGLRPE"
     gene            complement(905..1276)
                     /locus_tag="AC16CX_15970"
     CDS             complement(905..1276)
                     /locus_tag="AC16CX_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3014041.1"
                     /translation="MDLGRVHLVAQIGLLPVIVRKRWGAALGAAEINFIRALAPFQKF
                     ELVTRLVTWDEKYAYMEQRFESNGALCAHAFVKGLFLDANGKVASSAVAAELGVTESP
                     PPMPEELRIWAELGSVKKQKA"
     gene            complement(1286..1429)
                     /locus_tag="AC16CX_15975"
     CDS             complement(1286..1429)
                     /locus_tag="AC16CX_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014042.1"
                     /translation="MNLYLRLFVLLFRLIGLPRKGPFEESRISFRVLPNDCDINLHMN
                     NGR"
     gene            complement(1514..>1806)
                     /locus_tag="AC16CX_15980"
     CDS             complement(1514..>1806)
                     /locus_tag="AC16CX_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR3014043.1"
                     /translation="YGAKTVFLARFVPIVRTFAPFVAGIGRMRYGRFLFYSFSGSVAW
                     VGSLTYAGYYFGTVPVVKQNLALVIAVIVLLSISPGIFEYLRHRGAGVSGKR"
BASE COUNT          334 a          603 c          560 g          309 t
ORIGIN      
        1 accggcgagc gcctcggcca gcagatcgtg atcgacaacc gcgcgggcgc gagcggcacg
       61 atcggagtcg agatggcggc gcgcgccacg cccaacggct acacgtgggg gctcggcacg
      121 accagcaccc acgcgctcgc cgccatcctc aatcccaagt tgggctacga tccggcccgg
      181 gatttcgcgc cggtcgtggt gctgggcgac tccgcttatt tcctgaccac gaatcccacg
      241 ctgccggcaa ccaatatccg ggagttcatc gcgcatgcgc gcgccaatcc cggcaagctg
      301 cactacgctt ccgtcggcaa cttcagtctc gggcatctct ccggcgagct gctcaagaaa
      361 gcagccggca tcgacatggt gcacgtccct tacaagggat ccacgctagc gttgatcgac
      421 ctgctggccg gccgtatcca gttgaacatc agcaccattg cggcgtcgtt gccgcaagtc
      481 aaggccggga agctgcgtgc gctggccgtc accagccgcg agcgggtttc cgcgctgccc
      541 cagaccccga ctgtcgcaga atcggggttt cccggctatc aagcctcgct ctggatgggc
      601 gtattcgttc cggcgcgcac gcccggcgcg gtggtcgcgg ctctcaaccg cgagcttggc
      661 tcgaccttgc gctcggccga gctgcgcgag gcgctggccg agcaggccgt cgagcccggc
      721 gccggggcgc ccggggccct ggcgaagctc ttgcgggaag acaccgcgcg ctggcgcaag
      781 gtcattcagg acgcggggct gcgtcctgaa tgacccgtga taggtgataa gtgatagggg
      841 ataggtgaca agtgtttaac accggcagtg ccgggaactc ttatcactta tcactcgtca
      901 cttatcacgc tttttgcttt ttgacgctgc cgagctcggc ccagattctc agctcctcgg
      961 gcatgggcgg cggcgattcc gtaacgccca gctccgccgc caccgccgag ctcgccacct
     1021 tgccgttcgc gtccaggaac agccctttca cgaaggcatg ggcgcacagc gcgccgttcg
     1081 attcgaagcg ctgctccatg taggcgtatt tctcgtccca ggtcacgagc cgcgtcacca
     1141 gctcgaattt ctggaacggc gcaagcgcgc ggatgaagtt gatctccgcc gccccgagcg
     1201 ccgcccccca gcgcttcctg actatgaccg gcagcagccc gatctgcgcg acgagatgca
     1261 cgcgcccgag gtccatgaac gagagttagc gtccgttgtt catgtgcaaa ttgatgtcgc
     1321 agtcgttggg cagcacccgg aatgaaatgc gcgactcctc gaacggcccc ttgcgcggca
     1381 gcccgatcag gcggaacagc aggacgaata accgcagata gaggttcata ccgaatggcc
     1441 aagaccgtaa gcggtgagcg gtcagccgtt agcggtaata cccaatgtta ctggcgcttt
     1501 tcccgctaac cgcttaccgt ttaccgctta cccctgctcc gcggtgacgg aggtactcga
     1561 aaatccctgg cgagatcgaa agcagcacga tgacggcgat caccagcgcc aggttctgct
     1621 tcaccaccgg cacggtgccg aagtagtagc ccgcgtaggt gagcgatccg acccaggcga
     1681 cgctgcccga gaagctgtag aacaggaaac gcccgtagcg catgcgcccg atgcccgcga
     1741 cgaacggcgc gaaggtgcgg acgatcggaa cgaagcgcgc gaggaacacc gttttcgcgc
     1801 cgtact
//
LOCUS       DBNGWZ010000523        22265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1456123, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000523 DBNGWZ010000000
VERSION     DBNGWZ010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 22265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22265
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1456123"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /gene="proB"
                     /locus_tag="AC16CX_15985"
     CDS             <1..1107
                     /gene="proB"
                     /locus_tag="AC16CX_15985"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR3014044.1"
                     /translation="LETAKRLVVKVGSGLVTDRGRGLDLAALSRWAEQVAELRRMGRQ
                     VVLVSSGAIAAGMQRLGWVRRPHALHELQAAAAVGQMGLIQAYETCFRKHQLMTSQIL
                     LTHEDLSDRKRYLNARSTLRTLLGLGVVPIINENDTVAIDEIRFGDNDTLAALVTNLV
                     EADALVILTDQAGLYDRDPRKDGAATLVTEARAGDPALEAMAGGSGSHIALGGMLTKV
                     LAAKRAARSGAHTVIASGHEDNVLTRLARGERIGTQLFAERATLAARKQWLADHLQVH
                     GSLTLDAGAVRALTMQGKSLLPIGVFEVSGEFERGEAVSCIDESRREIARGLVNYSAQ
                     ETRRILRTPSGEIEAKLGYVDEPELIHRDNLVLL"
     gene            complement(1203..1736)
                     /locus_tag="AC16CX_15990"
     CDS             complement(1203..1736)
                     /locus_tag="AC16CX_15990"
                     /inference="COORDINATES: protein motif:HMM:NF020331.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HIR3014045.1"
                     /translation="MRVDALLLVTLLAALAAPPARAQWRQWDSDFDEDKKPWKEIESK
                     LPAYPKPENLIPFEAGGASPHRFFIDAQSLSIGEDGVARYTLVIKAVGGATNVTFEGI
                     RCEERQQKYYALGRPDGSWVRARNPQWRRIEYQEVNRHHNVLFQDFLCTGPARKSPVS
                     SVKEVLQLLKYGAPAGQ"
     gene            complement(1726..2934)
                     /locus_tag="AC16CX_15995"
     CDS             complement(1726..2934)
                     /locus_tag="AC16CX_15995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017685909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014046.1"
                     /translation="MLESQLAERIAQRLRELKLPIGVELWDGRSVTPAVQPRVRLSIR
                     SPKALAALLNPSMGKLARHYVEQNIDVEGDAREVVRLSEALSAASAGPGEANSGVRRP
                     ARHSRLFDRDAIRYHYDVKDEFYGLWLDRRRVYSCAYFRGSDDTLDTAQEQKLDLICR
                     KLMLKPGERFLDIGCGWGALVMWAAQRFQARATGITLSRDQHDFARARIREAGLEGLC
                     EVRLLDYRDVPEDEPYDKIASVGMFEHVGRKNLPMYFGKIFRLLKPGGVVMNHGITLT
                     SPEQSELGSDIGAFIDEYVFPGGELTHISQVIGAMSAQGLECWDVENLRPHYARTLWQ
                     WVERLDANRAAALAAVGERLYRIWRIYMAGSAHAFERGWLSVYQVLAGRPLTDGTPGF
                     PQTRDWIYAR"
     gene            complement(3038..3625)
                     /locus_tag="AC16CX_16000"
     CDS             complement(3038..3625)
                     /locus_tag="AC16CX_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HIR3014047.1"
                     /translation="MPRVTPDSLMTLEAYARARPEFRARVMAHKKDRTLALGEHVTLI
                     FEDELTIRYQIQEMLRAERIFEAQGIQDELDAYNPLIPDGGNWKATMMIEYPDVDERQ
                     RMLARLIGVEDRVWVQVEGHARVYAIADEDLERENAEKTSSVHFLRFELDRAMAEALK
                     RGARLSAGIDHPQYRVAVNEVAPGVRDSLAGDLGG"
     gene            complement(3727..5055)
                     /locus_tag="AC16CX_16005"
     CDS             complement(3727..5055)
                     /locus_tag="AC16CX_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004190664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3014048.1"
                     /translation="MTVREGSLEAPTRHPLDWKSADFHDEAKLEQELRRIFEICHGCR
                     RCVNLCTAFPTLFDLVDSAETMEVDGVRKQDYWKVVDQCYLCDMCYMTKCPYVPPHPW
                     NVDFPHLMLRAKAVRFRRHGARFRDRLLSSTDAVGRLATIPVVVQFVNAANKTGLARG
                     LMQRALGVHKDRVLPDYAGMKFRGNARANASFPVRDGKNTPGKVAIFSTCYVNYNEPG
                     IGHDLLKVLEHNEVPATLVDKEACCGMPKFELGDLEAVARNKGVNVPVLARLAREGYA
                     ILTAVPSCTLMFKQELPLMFPDDAEVKAVAEAMFDPFEYFVARYKDGLLKTDFTKPLG
                     RVSYHVPCHLRVQNMGQKTRELLERIPDTRVTTVERCAGHDGTWGVKEEYFENSMKIG
                     RPVFRQMAEAGPDYVSSDCAIAGRHILQGMGDTKAQKQHPITLIRIAYGL"
     gene            complement(5153..5572)
                     /locus_tag="AC16CX_16010"
     CDS             complement(5153..5572)
                     /locus_tag="AC16CX_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HIR3014049.1"
                     /translation="MQLKGSKTHENLKAAFAGESQANRRYLYFAAKADVEGQNDVSAV
                     FRSTAEGETGHAHGHLEYLESCGDPATNLPFGKTRENLKTAIAGETHEYTDMYPGMAK
                     TARQEGFNEIADWFETLAKAERSHANRFQKALDALAD"
     gene            complement(5782..6375)
                     /locus_tag="AC16CX_16015"
     CDS             complement(5782..6375)
                     /locus_tag="AC16CX_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3014050.1"
                     /translation="MLRDYFTLPSVMLQPGVGYAQPTQVVPEKVTLDDPATSVMTDLT
                     RVTAVIILPGDSVDEAQRRMIQRGVRLLLVVDQDRKVVGLVTATDILGEKPVLAASER
                     GLRREEVLVRDVMTPQERLEVLNMADVRAAKVGHIVATLKKAGRLHTVVVDHDAKGRQ
                     VVRGLYSATQIARQLGAAIQTSEIARTFSEIESMLAR"
     gene            complement(6537..7079)
                     /locus_tag="AC16CX_16020"
     CDS             complement(6537..7079)
                     /locus_tag="AC16CX_16020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HIR3014051.1"
                     /translation="MIDRSGYRPNVGIVLCNWRNEVFWGKRVRQHAWQFPQGGIKHGE
                     TPEAAMYRELKEEVGLEPHHVHILGRTRGWLRYDVPEQWIRREWRGSYRGQKQIWYLL
                     RLIGRDCDVRLRASSKPEFDAWRWNDYWIQLDAVIEFKREVYQRALEELAPYLKRRSR
                     RDTHGRRVTRPEPADALDRG"
     gene            complement(7181..7981)
                     /locus_tag="AC16CX_16025"
     CDS             complement(7181..7981)
                     /locus_tag="AC16CX_16025"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR3014052.1"
                     /translation="MNFFSRWRWRRTLADAGSVYVTERFGVRSLHIGSDTIQSSMRLA
                     RPNDLELAYTRSMMGFLLFHPAPRRVLLVGLGGGSLVKFIYHRMPETVSEVLEVNPDV
                     VAIARRFFQVPASDGRLVIRVGDGAEFVARAGPGYDAILVDGYDGDSQVEELSSRAFY
                     GACLRRLHAHGVLVVNLWGSDRRFNDYLVRIETTFPAGTLCLPAEKPGNIITFAFRDP
                     PGDPRWDTLEARARELEARFGLEFGKFVRGLRKMNRYDDERLHVSDQP"
     gene            8039..9751
                     /locus_tag="AC16CX_16030"
     CDS             8039..9751
                     /locus_tag="AC16CX_16030"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3014053.1"
                     /translation="MRVTQFLLSTLKETPADAEVVSHRLMLRAGMIRQLARGIYTWMP
                     LGLRVLRKVEAVVREEMNAAGAIELTMPAVQPAELWQESGRWREYGPELLRIKDRHQR
                     DFCFGPTHEEVVSDLVRQEVRSYRQLPLNLYQIQTKFRDEIRPRFGVMRAREFVMKDA
                     YSFHADRASLDETYRRMHDCYSRIFTRLGLKFRAVAADTGAIGGTGSDEFHVLADSGE
                     DAIAYCPGSDYAANVELAEAVAPAAPRPKPAARLERVATPGVKTIADLCAFLKIPAER
                     TVKAVVVDGAGGQPALLMVRGDHELNLAKAGKLEQVRKPVAFSSAEAIRALFGADPGS
                     LGPVGFKGVVIADRTVAAMADFAVGANQNGHHYTGANIGRDFPEPVVADIRNVVAGDA
                     SPDGRGTLEILRGIEVGHIFQLRTKYSSAMNLTFLDEDGKPKIMEMGCYGIGVSRIVA
                     AAIEQNHDERGIIFPRALAPFELALVPIGMGKSARVREVAETLYRDLAAAGIDVLLDD
                     RDERPGVMFADMELIGIPHRLVVGERGLKNGQIEYQGRRDTQAQAVAFQDAVKSVKSK
                     VCGE"
     gene            9739..10377
                     /locus_tag="AC16CX_16035"
     CDS             9739..10377
                     /locus_tag="AC16CX_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3014054.1"
                     /translation="MRGIAHVVFRLGWALVAAAPAAIYAGAQAYEPLSASVQAALNKS
                     ISDQATPFLAFSTEVEARRWLTAMSKRLASRMPDRLRREEFLVTVHYEAKRAGLDPQM
                     VLGLIQVESNFRKYAVSRAGARGYMQVMPFWMNLIGKRDDNLFHLRTNLRYGCVILRH
                     YLDRESGDLYRALGRYNGSLGKPEYPTQVVRAWQRDWHYATRTAETARERSS"
     gene            complement(10455..11717)
                     /locus_tag="AC16CX_16040"
     CDS             complement(10455..11717)
                     /locus_tag="AC16CX_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HIR3014055.1"
                     /translation="MLKLRKTPPLLDFVRMAWRRFHEERCLQVASSLTFTALLATVPI
                     ITVALTMISAFPVFRELMQHVQQFLVKNMLPESAETIAVYAEQFADNAARLTAVGILF
                     LFVTAMIVLQTIDRALNQIWRVPRPRTAVQRAFIYWALLTVGPLLIGASLSLTSWLVS
                     QSLGLVKDVPLAGEALLKVVPIGLTGLAFSLLYIALPNRRVLVRDALSGGFLAALAFE
                     GMKHGFAFYVTHFPTHKLVYGAFASVPVFLLWIYLSWLVVLFGAVSAAVMPEWRERAS
                     QVESVPGAQFLDALQILRLLRDAHRTGDIVTVFHLHDVIKLPIDRIEAILDAMSGARW
                     TGKVAHGWALTKDAAEISVADVYHLFVFRAGARLPTRQSGQELDRLALELAGGIEDGL
                     RLSLEDLFHKVALETATAIPKRIQAIQG"
     gene            11858..12451
                     /gene="wrbA"
                     /locus_tag="AC16CX_16045"
     CDS             11858..12451
                     /gene="wrbA"
                     /locus_tag="AC16CX_16045"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR3014056.1"
                     /translation="MKEILVLYYSHTGAVKEMAQLVARGVESIPGCAARVRTVPKVSA
                     VREAAGPAVPEAGAPYAELKDLEECIGLALGSPTRFGNMAAPMKYFWDSTGELWLKGR
                     LAGKPAAVFTSTASLHGGQETTLVSMMLPLLHHGMVIVGLPYSEPELMTTSGGGTPYG
                     ASHHAGAAGERAVTEHERKLCIALGRRLAEIAIKLAR"
     gene            12555..13364
                     /locus_tag="AC16CX_16050"
     CDS             12555..13364
                     /locus_tag="AC16CX_16050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025252.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HugZ family pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3014057.1"
                     /translation="MSHGEQARRYLRARRHGVLSTLSRKLEGYPFGSVAPFMLDPAAR
                     PVVLISRLAEHTKNIEADSRVSLLVSDAGDDVQAGARLTLIGDAARAGGDLDALRSRY
                     LSYFPDAGRLLALGDFSFYAIEPRQLRYIGGFGDIHWISAETYFPPTSHLAEQEQDIV
                     AHMNSDHAHNLRDCCRHYHGKTVREARMIGIDCDGFDLRADGVRPLIRDGQASEDAAP
                     AACPPSGSHPARRALAPPSGVLLRFDFEQPVTDAAQARGALIAMARKARAA"
     gene            complement(13639..13839)
                     /locus_tag="AC16CX_16055"
     CDS             complement(13639..13839)
                     /locus_tag="AC16CX_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR3014058.1"
                     /translation="MAKWLITIGIVLVALGLAWPLLAKLGLGSLPGDIKFERKGFTFY
                     FPITTGLIISALITLVLWIFRR"
     gene            13968..14801
                     /locus_tag="AC16CX_16060"
     CDS             13968..14801
                     /locus_tag="AC16CX_16060"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR3014059.1"
                     /translation="MPLIAMTREMGSLGKDVAAALADRLGKQVVHHEIIDNLASKMRL
                     RKSHVIRFLEGKSGIWERLTTDKTSLSIYTADETFALAESGNVAVIRGWGATHLLRPV
                     QHVISVRVCAPFEVRVKRMLERLNTDDRDFVENEIRLSEEAHGAITRRHFGINWQDSE
                     HYDLVLNTERLTVEECADEVTALLDDSTFQETAQSQRYFRNLALAAHVRAALRQDVRT
                     SKMMFSITCDDGVATLAGLVDPGQEPKDAVEVASKVPGVKEIRNQLRVVTAGARHHVE
                     G"
     gene            complement(14864..15850)
                     /locus_tag="AC16CX_16065"
     CDS             complement(14864..15850)
                     /locus_tag="AC16CX_16065"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3014060.1"
                     /translation="MRQSKPVAAALICAATLLATGGAHAQYASELGKLAPEALKGGAK
                     PQPAQLKRIAPDLYFFWNDGSSNAVFMVTDEGVMVVDTQQHPADARMLLGMIRKITDQ
                     PIKWVVVTHPHGDHFLGNPVFKSEGATIVSHRDTRGMMQKYYKDEVQRRQAYFKRYDL
                     DPGELQMVLPEVTFDSRFTIHLGGRTAELIHLGPGQNPGDTLIHFPHARTLFLGGPFA
                     RRNWSNYTFTPSVEGWIALLRKAAAFDADTFLGGHGDVSVREDLLEYAQMHEDFLKAV
                     RAGIAEGKNRDELADTIRMQQYSHYRNYHRMRGWVYALHHLLTTGKPMAPYP"
     gene            complement(15888..17429)
                     /locus_tag="AC16CX_16070"
     CDS             complement(15888..17429)
                     /locus_tag="AC16CX_16070"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3014061.1"
                     /translation="MAKEKLIIFDTTMRDGEQSPGASMTKEEKLRIARQLERLHVDVI
                     EAGFPAASDGDFDAVRAVASIIKDSTVCGLARANENDIRRCGEAVKPAKSPRLHTFIA
                     TSPIHMEKKLRMEPSQVIEQAVKAVKWARQYTDNVEFSPEDAGRSDIDFLCRILERVI
                     RAGATTINIPDTVGYTFPEHFGRLIRLLRERIPDSDKVVWSVHCHNDLGLAVANSLAA
                     VLNGARQVECTINGLGERAGNAALEEIVMAVRTRQDIFPCDTNIDTTQIVPASRLVSG
                     ITGFPVQPNKAIVGANAFAHEAGIHQDGVLKSRDTYEIMRAEDVGWNANRLVLGKHSG
                     RNAFRQRLQEIGIMLDSEEALNAAFKRFKDLADKKHEIFDEDLQALISEQASDYVENE
                     HYKLVSLTAHSETGEAPYAKIVVAEDGRERRAQAQGSGPVDASFKAIESIVNSGSELL
                     LYSVNAITTGTDAQGEVTVRLTKGGRIVNGQGADTDIVVASAKAYINALNKLHSKLER
                     INPQV"
     gene            complement(17817..18650)
                     /gene="pssA"
                     /locus_tag="AC16CX_16075"
     CDS             complement(17817..18650)
                     /gene="pssA"
                     /locus_tag="AC16CX_16075"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR3014062.1"
                     /translation="MYEPREHKLFIQSRFARRGIYWLPNLFTTIALFAGFYAIVQAMG
                     GRFEQAAMAIFIAMVFDGLDGRIARLTRTQSAFGAEYDSLSDMVSFGVAPALVVYVWA
                     LKDFSGMKTVAWLGPWLTTKLGWIAAFIFCVCAALRLARFNTTLDVADKRFFQGLPSP
                     AAACLIAGLVWALNEYQIKGADVKWLAWGLTVFAGLTMVSNFKFYSGKDINLRKSVPF
                     SVVVGIAFGLVALFMLSSTLPEMLFLLFAGYSCSGYVIWVYEMVKGKGPGHTPPPPVA
                     S"
     gene            complement(18745..19389)
                     /locus_tag="AC16CX_16080"
     CDS             complement(18745..19389)
                     /locus_tag="AC16CX_16080"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR3014063.1"
                     /translation="MGDYPHPIIAREGWPFIAASIVAALLIHWLAGAWWAAPLWLGVA
                     FLVQFFRDPPRAIPQESKAVLSPADGRIVTVERARDPYLERDALKMSVFMNVFNVHSN
                     RSPVDGEVREAWHHPGKFFNAELSKSSLENERNALWIRTAGGADVTCVQIAGLIARRI
                     LCYARTGQRLTRGERYGFIRFGSRVDVYLPPESRPVASMGDKVYATESILAMLP"
     gene            complement(19469..20485)
                     /gene="ilvC"
                     /locus_tag="AC16CX_16085"
     CDS             complement(19469..20485)
                     /gene="ilvC"
                     /locus_tag="AC16CX_16085"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR3014064.1"
                     /translation="MKIYYDKDADLSLIKHKHVAIIGYGSQGHAHANNLKDSGVRVTV
                     GLRKGGASWTKAKNAGLPVKPVGDAVKSADIVMILLPDEHHAQVYSDEIEPNIKKGST
                     LAFAHGFNIHFKQIEPRADLDVIMIAPKGPGHLVRSTYTQGGGVPSLIAVYQDPSRNA
                     RDVALSYAAAIGAARAGVIETTFREECETDLFGEQVVLCGGLTALIQAGYDTLVEAGY
                     APEMAYFECLHEVKLIVDLIYEGGIANMRYSVSNTAEYGDFTRGPRVINDAVRAEMKK
                     ILLEIQTGQFAKEFILENRAGAPSLKAMRRMAASLPVEIVGARLRDMMPWIKKNRLVD
                     QTKN"
     gene            complement(20591..21082)
                     /gene="ilvN"
                     /locus_tag="AC16CX_16090"
     CDS             complement(20591..21082)
                     /gene="ilvN"
                     /locus_tag="AC16CX_16090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3014065.1"
                     /translation="MRHIVSILLENEAGALSRVAGLFSARGYNIESLTVAPTEDATLS
                     RMTIVTTGSDEVIEQITKQLNKLIDVVKVIDLSDGDHIERELMLVKVRAVGKDREEMK
                     RMADIFRGRIIDVTDRDYTIELTGTGSKLDAFLKALDRAAILETVRTGASGIGRGDRV
                     LRV"
     gene            complement(21079..>22265)
                     /locus_tag="AC16CX_16095"
     CDS             complement(21079..>22265)
                     /locus_tag="AC16CX_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3014066.1"
                     /translation="HYPESITMRSYNPVVRGHAGQIKKAIQALMKAKRPMIYTGGGVI
                     LSGASEELAQLVRLLGFPCTNTLMGLGAYPATDRQFLGMLGMHGTYEANMAMQHCDVL
                     LAIGARFDDRVIGNPRHFFAEPRTIVHVDIDPSSISKRVKVDIPIVGNVRDVLREFLR
                     QLEASKDGPDQEALKTWWTQIELWRSRDCLKYDRSSPLIKPQYVIEKLYEVTRGDAFI
                     TSDVGQHQMWAAQFYRFEKPRRWINSGGLGTMGFGLPAAMGVQLAHPKETVACVTGEA
                     SVQMCIQELSTCKQYRLPIKVVNLNNKYMGMVRQWQEFFHGNRYAESYMDALPDFVRL
                     AEAYGHVGMKIEKPEDVEPALKEAFARKQDLVFMDFITDQTENVFPMVPGGKGLSEMI
                     LV"
BASE COUNT         3784 a         7285 c         7257 g         3939 t
ORIGIN      
        1 ctggaaacag cgaaacggct ggtcgtcaag gtcggctcgg gcctggtcac cgaccggggg
       61 cgggggctgg atctcgccgc gctctcgcgc tgggcggagc aggtcgcgga gctgcggcgg
      121 atgggacgcc aggtggtgct ggtttcgagc ggcgcgatcg ccgcggggat gcagcggctc
      181 ggctgggtcc ggcgcccgca cgcgctgcac gagctgcagg cggcggcggc ggtggggcag
      241 atggggctga tacaggccta tgaaacctgt ttccgcaagc accagctgat gacttcccag
      301 atcctgctca cgcacgagga tctctccgat cggaaacgct atctcaacgc tcgctccacg
      361 ctgcgcaccc tgctcgggct gggcgtggtc cccatcatca acgagaacga tacggtcgcc
      421 atcgacgaga tccgtttcgg ggacaacgac acgctcgcgg cgctggtcac caacctggtc
      481 gaggccgacg cactggtgat cctcaccgac caggccggtc tctacgaccg cgacccgcgc
      541 aaggacggcg ccgcgaccct ggtcacggag gcccgggcgg gtgaccccgc cctcgaggcg
      601 atggcgggcg gcagcggcag tcacatcgcc ctcggcggca tgctcaccaa ggtgctcgcc
      661 gcgaagcgcg ccgcgcgcag cggcgcgcac accgtgatcg cctcggggca cgaggacaac
      721 gtcctgaccc ggctcgcgcg cggcgaacgg atcggcacgc agctcttcgc ggagcgcgcc
      781 accctcgcgg cgcgcaagca gtggctcgcc gaccacctcc aggtccacgg cagcctcacg
      841 ctcgacgccg gcgccgtgag ggcgctcacg atgcagggca agagcctgct tccgatcggt
      901 gtgttcgaag tgagcgggga gttcgagcgc ggcgaggcgg tgagctgcat cgatgagtcg
      961 cggcgcgaga tcgcgcgcgg tctcgtcaac tacagcgcgc aggaaacgcg ccgcatcctg
     1021 cgcaccccgt ccggcgagat cgaagcgaag ctcggctacg tggacgagcc cgaactcata
     1081 caccgcgata accttgttct gctgtaggca gaacgagatt gatgcgcgcc tttccctggt
     1141 gatcgggata agcaggcggt cgaacgagga gcttatcgtg cgctgatgct gccgcctgac
     1201 gcttactgac cggcgggggc gccgtatttc agcagttgca acacttcctt gacgctgctc
     1261 accggcgatt tgcgtgccgg gccagtgcac aggaagtcct ggaacagcac gttgtgatgg
     1321 cggttcactt cctggtactc gatccggcgc cattgcgggt tgcgcgcccg cacccagctg
     1381 ccgtccggcc gtcccagcgc gtaatatttc tgctggcgct cctcgcaccg tattccctcg
     1441 aaggtgacgt tggtggcgcc gcccacagcc ttgatcacaa gggtatagcg cgccaccccg
     1501 tcctccccga tcgacagcga ttgcgcgtcg atgaagaaac ggtgcgggct cgcgccgcct
     1561 gcctcgaatg gaatcaggtt ctcgggtttg ggataggccg gcagcttcga ctcgatttcc
     1621 ttccacggtt ttttatcctc gtcgaagtcg ctgtcccact gccgccactg cgcccgggcc
     1681 ggcggcgccg ccagcgcggc gagcagcgtc acgaggagga gcgcatcaac gcgcataaat
     1741 ccagtcccgc gtttgcggaa atcccggcgt gccgtcggtc aagggcctgc ccgcgagcac
     1801 ctggtagacc gacagccacc cgcgctcgaa cgcgtgcgcc gagccggcca tgtagatgcg
     1861 ccatatgcgg tagagccttt cgccgacggc cgccagcgcc gcagcccggt tcgcgtccag
     1921 ccgctccacc cattgccaca gcgttctcgc gtaatgcggc cgcagattct ccacgtccca
     1981 gcattcgagg ccctgcgcgg acatcgcccc gatcacctga gagatatggg tgagttcccc
     2041 gcccggaaaa acgtattcgt cgatgaacgc gccgatgtcg ctgccgagct cgctctgttc
     2101 gggcgaagtg agcgtgatgc cgtggttcat gacgacaccg ccgggcttga gcaggcggaa
     2161 aatcttgccg aaatacatag gcaggttttt cctgccgacg tgctcgaaca tgccgacgct
     2221 ggcgattttg tcgtagggct cgtcttccgg cacgtcacga tagtcgagga gcctcacctc
     2281 gcacagccct tccagccccg cctcccggat gcgggcccgc gcgaaatcgt gctgatcccg
     2341 cgagagcgtg atgcccgtgg cgcgcgcctg gaagcgctgc gcggcccaca tgacgagcgc
     2401 gccccagccg cagccgatgt ccagaaaccg ctcgccgggc ttgagcatga gcttgcggca
     2461 gatgaggtcg agtttctgct cctgggcggt gtcgagggtg tcgtcagacc cgcggaaata
     2521 ggcgcaggaa taaacgcggc gccgatcgag ccacaggccg tagaactcgt ctttgacgtc
     2581 gtagtgatag cgtatcgcgt cgcgatcgaa cagccgggaa tgccgcgccg gtctgcggac
     2641 acccgaattc gcttcgcccg gtccggccga cgcggcgctc agcgcttcgc tcaagcgcac
     2701 cacttcgcgc gcgtcgcctt cgacgtcgat attctgctcg acataatggc gggcaagctt
     2761 gcccatgctg ggattgagga gcgcggcgag cgccttggga gagcgtatgc tcaacctgac
     2821 gcgcggctgg accgccggcg tgaccgacct tccatcccat agctcgacgc cgatcggcag
     2881 cttgagctcg cgcaagcgtt gcgcgatacg ttcggcgagt tgtgattcca gcatggggtc
     2941 actccttcag ggatcccgca cccagcataa gtaaggtgcc gcaacggccg gcgggttgcg
     3001 ctggatcaaa tcagaatagc atcgctcgcc cgggcagtca gccccccaga tcgcccgcaa
     3061 gcgaatcccg gacccccggg gcgacctcat tgaccgcgac gcggtattgc ggatggtcga
     3121 tgccggctga caggcgcgcg ccgcgcttga gcgcttcagc catggcgcga tcgagctcga
     3181 agcgcaggaa atgcaccgat gaggtcttct cggcgttttc gcgctcgagg tcctcgtcgg
     3241 cgatggcgta gacccgggca tggccctcga cctgcaccca gacccggtcc tcgacgccga
     3301 tcagccgggc gagcatccgc tgccgttcat ccacgtcggg atattcgatc atcatcgtcg
     3361 ccttccagtt gccgccgtcg gggatgagcg gattgtaggc atccagttcg tcctgtatgc
     3421 cttgcgcctc gaatatgcgc tcggcgcgca gcatttcctg gatctggtag cggatggtga
     3481 gctcgtcctc gaaaatgagc gtgacgtgct cgccgagggc gagcgtgcgg tccttcttgt
     3541 gcgccattac gcgcgcgcgg aattctggcc gcgcccttgc gtaggcttcc agcgtcatca
     3601 ggctgtcggg ggtgactcta ggcataactg cgtgaatggt gattggtgaa tagtgagtag
     3661 tcttgattcg tgagcggccg ttcgcagaag tgattcagtc cactcactac tcacgactca
     3721 cgctgttcag agcccatagg cgatgcgtat cagcgtgatc gggtgctgct tctgtgcctt
     3781 cgtgtcgccc atcccctgca ggatatggcg gccggcgatg gcgcagtcgg agctcacgta
     3841 atcgggaccg gcttccgcca tctggcggaa caccggccgg ccgatcttca tcgagttttc
     3901 gaagtactcc tccttcacgc cccaggtgcc gtcgtgcccg gcgcagcgtt cgacggtggt
     3961 gacccgggtg tcggggatcc gctcgagcag ctcgcgcgtc ttctgcccca tgttctgcac
     4021 ccgcaggtgg caaggcacgt ggtaggagac ccggcccagc ggcttcgtga aatccgtctt
     4081 gagcaggccg tccttgtagc gggccacgaa gtactcgaag ggatcgaaca tcgcctcggc
     4141 caccgccttg acctcggcat cgtccgggaa catcaacggc agctcctgct tgaacatcag
     4201 cgtacaggaa ggaaccgcgg tgaggatggc gtagccttcg cgggcgagtc gcgcgagcac
     4261 cggcacattg acccccttgt tgcgagccac cgcttccagg tcgcccagct cgaacttggg
     4321 catgccgcaa caagcttcct tgtcgacgag cgttgccggc acttcgttgt gttcgagcac
     4381 cttcaacagg tcgtgtccga tgccgggctc gttgtagttc acgtagcagg ttgagaatat
     4441 cgcaaccttg cccggcgtgt tcttgccgtc gcgcaccgga aacgaggcat tggcacgggc
     4501 gttgccgcgg aacttcatgc cggcatagtc gggtagaacc cggtccttgt gcacgccgag
     4561 ggcccgttgc atgagtcccc gcgccagacc ggtcttgttc gccgcgttca cgaactgcac
     4621 cacgaccggg atcgtggcaa gcctgccgac ggcgtcggtg ctggaaagca gcctgtcgcg
     4681 gaaccttgcc ccgtgcctgc ggaacctgac cgctttggcg cgcagcatca agtgggggaa
     4741 atccacgttc cacgggtgcg gcggcacgta cgggcatttg gtcatgtagc acatgtcgca
     4801 caggtagcac tggtcgacga ccttccagta gtcctgtttc ctgaccccgt cgacttccat
     4861 cgtctcggct gagtccacta ggtcgaacag ggtcgggaaa gcggtgcaga gattcacgca
     4921 gcggcggcag ccgtggcaga tctcgaagat acgccggagt tcctgctcga gcttggcctc
     4981 gtcgtggaaa tccgccgatt tccagtcgag cgggtgccgg gtgggagctt ccaggctccc
     5041 ttcacggacg gtcacgttga gtgaggagag aagagtaagg agtgaggagc aatgcggcgc
     5101 aaggatctct cctcacgcct cacgcctcac ccctcacttc atattggcgg actcagtcag
     5161 ccagtgcgtc gagggccttc tggaagcggt tggcgtgcga gcgctccgcc ttggccagcg
     5221 tttcgaacca atcggcgatc tcgttgaagc cttcctggcg cgcggtcttg gccatgcccg
     5281 ggtacatgtc ggtgtactcg tgggtttcgc cggcgatcgc cgtcttcaga ttctcgcgcg
     5341 tcttgccgaa cgggagattg gtcgctggat caccacagga ttccagatac tcgagatgcc
     5401 catgggcgtg cccggtttcg ccttcagcgg tagagcggaa cactgcggac acgtcgttct
     5461 ggccctcgac gtcggccttc gcagcgaaat acaggtagcg ccggttggct tgggattcgc
     5521 cagcaaacgc ggctttcaga ttctcgtgcg tcttggatcc tttgagttgc atataactct
     5581 cctattaatc aatcagctaa acgcatactt aggcgtcatg gaagggctta ctgacctgcg
     5641 cctgtaaaac cggaattaga ctgagtctaa cctgttaact gagtgtaagc agcggctccg
     5701 tcactgtcaa tagcgtcctg gcgcgaaatt aacgtgaatc aatgcagtaa ccgtcacgcc
     5761 ccggtcgcgg cagcaggcca tctagcgcgc cagcatggac tcgatttccg aaaaggtgcg
     5821 cgcaatctcg ctggtctgga tcgccgcgcc gagctggcgg gcgatctggg tagccgaata
     5881 aaggcctctt acgacttgcc ggcctttggc gtcgtggtcc acgacaacgg tgtgcaagcg
     5941 tcccgctttc ttgagggtgg caacgatgtg cccgaccttg gctgcgcgca cgtccgccat
     6001 gttgagcacc tcgaggcgct cctggggggt catgacgtcg cgcaccagca cctcctcgcg
     6061 gcgcagcccg cgctcggagg cggccagtac cggcttctcg ccgaggatat cggtggccgt
     6121 gaccaatccc accaccttgc ggtcctggtc gacgacgagg agcagccgca ccccgcgctg
     6181 gatcatgcgc cgctgtgcct cgtccacgct atcgccgggc aggatgatga ccgcggtgac
     6241 ccgggtgaga tcggtcatta cgctcgtcgc cgggtcatcg agcgtgactt tctcgggcac
     6301 gacctgggtt ggctgggcat aacccacgcc cggctgcagc atgacggagg gcaaggtgaa
     6361 atagtctcgc agcacggctt cctcctctgt ctgatactgc tggaatccga tagtttagat
     6421 cagctcaagg atgatggcat cccggggttt cggggaaacc ccgacctcag gccgtgccgg
     6481 ggacacgacg tcgcgccggc gtgaggagac ggcgctgctg cggcagccgc tgccgctcag
     6541 ccccgatcca gcgcgtccgc cggctcggga cgtgtgactc tcctcccgtg cgtatcgcgt
     6601 ctggaacggc gtttgagata aggggcgagt tcctcgagcg ccctctggta gacctcgcgc
     6661 ttgaattcga tcactgcgtc cagctgaatc caataatcgt tccagcgcca ggcgtcgaat
     6721 tcgggcttgc tcgaagcgcg cagccgcacg tcgcagtcgc ggccgatcaa ccgcagcaag
     6781 taccagatct gcttctggcc gcggtagctg ccgcgccatt cccgccggat ccactgctcg
     6841 ggcacgtcgt agcgcagcca gccgcgcgtg cgcccgagga tgtgcacgtg atgcggctcg
     6901 agccccactt cttccttcaa ttcgcgatac atcgcggctt ccggggtctc gccatgcttg
     6961 atgccgcctt gcgggaactg ccaggcgtgc tgtctcaccc gcttgcccca gaaaacctcg
     7021 tttctccaat tacataggac gatgccgacg ttggggcggt agccactgcg atcgatcatg
     7081 gtaacccccg ctaaaccacg ctttgacgcg attttttcac aattcggact caaaaaaaag
     7141 cccgcgcccg gatttccggc catccatcac cgggaacggg tcacggctgg tcggaaacat
     7201 ggagccgctc gtcatcgtac cggttcatct tgcgcaatcc ccgcacgaac ttgccgaatt
     7261 cgagcccgaa gcgcgcctcg agttcacgcg ccctggcctc gagcgtgtcc cagcgcggat
     7321 cccccggcgg gtcccggaac gcaaacgtga tgatattgcc cggcttctcg gccggcaggc
     7381 acagcgtgcc cgcgggaaac gtcgtctcga tgcgcacgag gtaatcgttg aaccggcggt
     7441 cggatcccca cagattcacc accagcacac cgtgtgcgtg gagtcgtcgc aggcacgcgc
     7501 cataaaacgc gcgggacgaa agctcctcga cctgcgagtc gccgtcgtag ccgtccacca
     7561 ggatcgcgtc gtagcccggc cctgcgcgcg cgacgaactc cgcgccgtcg cccacccgga
     7621 tcacgaggcg cccgtcggat gcgggcacct ggaaaaagcg ccgcgcgatc gccaccacat
     7681 cgggattgac ctccagcacc tcggaaaccg tctccggcat gcggtggtag atgaacttga
     7741 cgagcgagcc gccgcccagc cccaccagca gcacgcgccg cggcgcgggg tggaacagca
     7801 ggaatcccat catgctgcgc gtgtaggcga gctcaagatc gttcgggcgc gcgagtcgca
     7861 tcgagctctg gatggtgtcg ctgccgatgt gcagcgagcg cacgccgaat ctctccgtga
     7921 cgtacaccga cccggcgtca gcgagcgtcc ggcgccatcg ccatctggag aagaaattca
     7981 tcccgctggc agcagtgaac gggttggtag aatagcgttt tcgcggattt taacagccat
     8041 gcgcgtcacg caattcctgc tctccaccct caaggaaacg ccggccgatg ccgaggtggt
     8101 gagccaccgg ctgatgctgc gcgcgggcat gatccggcag ctcgcccggg ggatttacac
     8161 ctggatgcct ctgggcttgc gcgtgttgcg caaggtggag gcggtcgtgc gcgaggaaat
     8221 gaacgctgcg ggcgcgatcg agctcaccat gcccgcggtg cagcccgccg agttgtggca
     8281 ggaatccggg cggtggcgcg aatacggacc ggaactgctg cgaatcaagg atcgccatca
     8341 gcgcgatttc tgcttcgggc cgacgcacga agaggtggtg agcgacctcg tgcgccagga
     8401 ggtcaggagc taccggcagc tgccactcaa cctctaccag atccagacca agttccgcga
     8461 cgagatccgg ccgcgcttcg gcgtcatgcg ggcgcgcgag ttcgtgatga aggacgccta
     8521 ctccttccat gccgacaggg cgagcctcga cgagacctat cgcaggatgc acgactgcta
     8581 ttcccgcatc ttcacgcggc tgggactgaa gttccgtgcg gtcgcggccg acaccggcgc
     8641 gatcggcggc accggctcgg acgaattcca cgtgctggcc gactccggcg aggatgcgat
     8701 cgcatactgt ccgggctccg actacgccgc caatgtcgag ctcgcggaag cggtggcgcc
     8761 cgcggcgccg cgcccgaagc ccgccgcgag gctcgagcgc gtggccacgc cgggcgtgaa
     8821 gacgatcgcc gatttgtgcg cgttcctgaa gattcccgcc gaacgcaccg tgaaggcggt
     8881 cgtggtggac ggtgccggcg ggcagccggc gctgctcatg gtgcgcggcg accacgaatt
     8941 gaacctcgcc aaggccggca agcttgagca ggtgagaaag ccggtcgcgt tctcttccgc
     9001 cgaggcgatc cgcgcgctgt tcggggcgga ccccggctcg ctcggtccgg tcggcttcaa
     9061 gggtgtcgtc atcgccgatc ggacagtcgc ggcgatggcc gatttcgccg tcggcgccaa
     9121 ccagaacggc catcattaca ccggcgccaa tatcgggcgc gatttccccg agcccgtggt
     9181 tgccgacatc cgcaacgtgg tggcagggga tgccagcccc gacggcaggg gcacgctcga
     9241 gatcctgcgc gggatcgagg tcggccatat cttccagttg cgcaccaagt actcgagcgc
     9301 gatgaatctc accttcctcg acgaggacgg caagccgaaa atcatggaga tgggctgtta
     9361 cggcatcggc gtttcgcgca tcgtcgcggc cgcgatcgag cagaaccacg acgagcgggg
     9421 catcatcttc ccgcgtgcgc tggcgccgtt cgagctcgcg ctggtgccga tcgggatggg
     9481 caagagcgcg cgggtgcgcg aggtcgctga aacgctctat cgcgatctcg ccgccgccgg
     9541 catcgacgtg ctgctcgatg accgcgacga gcgccccggt gtcatgttcg ccgacatgga
     9601 gctgatcggc atcccccacc gtttggtggt cggggaaaga gggctcaaga acgggcaaat
     9661 cgaataccag gggcggcgcg atacccaagc ccaggccgtc gcttttcaag atgcggttaa
     9721 atcagtaaaa tcaaaggtgt gcggggaata gctcacgtcg tgttcaggct ggggtgggcg
     9781 ctcgttgcgg cggcgccggc cgctatctat gccggcgcgc aggcctacga gcccctgtcg
     9841 gccagcgtgc aggcggcgtt gaacaagtcc atttccgacc aggccacccc gttcctggcg
     9901 ttcagcacgg aagtggaagc gcggcggtgg ctcaccgcca tgtcgaagcg cctcgcctcg
     9961 cgcatgcccg accgcctgcg gcgcgaggag ttcctcgtca cggtgcacta cgaggccaag
    10021 cgcgcgggtc tcgatccgca gatggtgctg ggcctgatcc aggtcgagag caacttccgc
    10081 aagtacgcgg tctcgcgcgc cggggcccgg ggctacatgc aggtcatgcc gttctggatg
    10141 aacctgatcg gcaagcgcga cgacaacctg ttccacctgc gcaccaacct gcgctacggc
    10201 tgcgtgatcc tgcgccacta cctcgaccgg gaaagcggcg acctctaccg ggcgctcgga
    10261 cgctacaacg gcagcctggg gaagcccgaa tacccgaccc aggtggtgcg cgcctggcag
    10321 cgcgactggc attacgccac gcgcaccgcg gagaccgcgc gcgagcgttc ctcctgagtc
    10381 acctttccgc cgcggccgtt tccctcgctc cgcggctccc gcggcgtgag tggcggcgct
    10441 ctggcccgcg ccgcctatcc ctggatcgcc tggatgcgtt ttggaatggc ggttgcggtt
    10501 tccagggcca ccttgtgaaa caggtcctcc agggaaagcc gcaagccgtc ctcgatgccg
    10561 ccggcaagct cgagcgcgag gcggtcgagt tcctgtcccg actggcgtgt cggcagcctg
    10621 gcgcctgcgc ggaacacgaa caggtggtag acgtcggcca cgctgatctc ggccgcgtcc
    10681 ttcgtgagcg cccagccgtg cgccaccttg cccgtccagc gcgcaccgct catcgcatcg
    10741 aggatcgcct cgatccggtc gatcggcagc ttgatgacat cgtgcaagtg gaacacggtg
    10801 acgatgtcgc cggtgcggtg cgcatcccgt aacagacgaa ggatctgcag agcatcgaga
    10861 aactgcgcgc cgggaacgga ttcgacctgc gaggcgcgct cgcgccattc cggcatcacc
    10921 gccgccgaga ccgccccgaa cagcacgacc agccacgaga gatagatcca cagcaggaac
    10981 acgggaacgc tggcaaacgc gccatagacg agcttgtgag tcggaaaatg ggtgacgtag
    11041 aacgcgaagc cgtgcttcat cccctcgaac gcgagcgccg cgagaaatcc gccggagagc
    11101 gcgtcgcgca cgagcacgcg ccggttcggc agggcgatat agagcagcga aaatgcgagg
    11161 ccggtgagcc cgatcggaac gaccttgagc aacgcttcac ccgccagggg cacatccttc
    11221 accagcccca gggactggct gaccagccag gaagtcaggg acaggctcgc cccgatcagc
    11281 agcggtccca cggtgagcag cgcccagtag atgaacgcgc gctgcacggc ggtccgcggc
    11341 cggggcacgc gccagatctg gttcaaggcg cggtcgatcg tctgcaacac gatcatcgcg
    11401 gtgacaaaca ggaacaggat gccaacggcg gtgagcctgg ccgcgttgtc ggcgaactgc
    11461 tcggcgtaga cggcgatggt ctcggccgac tccggcagca tgttcttgac caggaactgc
    11521 tggacgtgct gcatcagctc gcggaacacc gggaacgccg agatcatcgt cagcgcgacg
    11581 gtgatgatcg ggacagtcgc gagcagcgcg gtaaaggtga ggctggaggc gacctgcagg
    11641 cagcgctcct cgtggaagcg ccgccacgcc atgcgcacga aatcgaggag cggcggggtc
    11701 ttcctgagct tcagcactat gctgccctca tggcaagacg gctttatata atactctctg
    11761 gcagcctgtc ggacttacgg gtgcgccagg ctgttacagc aggaccggct gacaattcgg
    11821 attttgccgt ccttggccct gacaccatcc tgtcgcgatg aaggaaatcc tcgtcctcta
    11881 ctacagccat accggcgcgg tgaaggagat ggcgcaactc gtcgcgcgcg gcgtggagag
    11941 cattcccggc tgtgccgcgc gcgtgcgcac cgtgcccaag gtttccgcgg tgcgcgaagc
    12001 ggccgggccc gcggtgccgg aagccggcgc gccgtacgcg gagctgaagg acctcgagga
    12061 gtgcatcggg ctcgcgctgg gctcgcccac ccgcttcggc aacatggcgg cgccgatgaa
    12121 atatttctgg gattcgaccg gcgagctgtg gttgaagggc aggctcgcgg gcaagcccgc
    12181 ggcggtgttc acctccaccg cgagcctgca tggcggccag gagaccaccc tcgtttcgat
    12241 gatgctgccg ctgctgcatc acggcatggt gatcgtgggc ctgccctact cggaacccga
    12301 gctcatgacc accagcggcg ggggcacgcc gtacggcgcg agccaccacg ccggcgccgc
    12361 cggcgagcgg gcggtcaccg agcacgagcg caagctctgc atcgcgctcg ggcggcggct
    12421 cgccgagatc gcaataaagc tagcgagatg agccagggag caggggcccg cttgcgggat
    12481 gcgaccaagg cgaacgctcg cggccgccga cgcgataagt ctggattcac agcttggctg
    12541 tccgaggcgg tgagatgagc cacggcgagc aggcgcggcg gtatctgcgc gcgcggcgtc
    12601 acggcgtgct gtccaccctt tcccgcaagc tcgagggtta tcccttcggc tcggtcgcgc
    12661 cgttcatgct cgacccagcg gcacgcccgg tcgtcctgat cagccgtctc gccgagcaca
    12721 ccaagaacat cgaggccgat tcacgggtca gcctgttggt gagcgatgcc ggcgacgatg
    12781 tgcaggccgg cgcgcggctg acgttgatcg gggatgccgc gcgggcaggc ggcgacctag
    12841 atgccctgcg gtcgcgctac ctgagttact ttcccgatgc cgggcggctg ctcgccctgg
    12901 gcgatttctc gttctacgcg atcgagccgc gccagctgcg ctacatcggc ggcttcggcg
    12961 acatccactg gatttcggcc gaaacatact tcccgcccac gagccacctt gcggagcagg
    13021 agcaggacat cgtggcgcat atgaattctg atcatgcgca caatctccgt gattgctgcc
    13081 gccactatca cggcaagacc gtgcgcgagg cgcggatgat cgggatcgac tgcgacgggt
    13141 tcgacctccg ggcggatggc gtgcggcctc tgatacgaga tgggcaagca tcggaggatg
    13201 ccgcgccggc cgcctgcccg ccttcggggt cgcacccggc acgccgggca cttgctcctc
    13261 cctccggcgt gctgctgcgc ttcgatttcg agcagccggt gacggacgct gcacaggcgc
    13321 gcggagcgct cattgcgatg gcgcgcaagg cgcgcgcggc atgaggaaat caagaacccg
    13381 gtgccgcgcg atacgctgct cttcggtccc gactgcttcg cgcgctgatt ccgcgcggcg
    13441 cgccgggacg ccagtccgaa gccggtccgt cttcgggaag ggggtttgtc ctgttgttcg
    13501 cggcttgaaa agctaacagg cggttgaaaa accacactgg gcgcgtccaa gcacgactgg
    13561 attcataccg gcttcgtcct caacagcctc tgacctaggg gatagaaagg gggcttcgcc
    13621 ccctttttca acgccgggct aacgccggaa gatccacagc acgagggtga tcaacgccga
    13681 gatgatcagg ccggtggtga tcggaaaata gaaagtaaac cccttgcgct cgaacttgat
    13741 gtcgccgggc aggctgccca ggccgagctt ggcgagcagc ggccaggcca ggcccagcgc
    13801 caccagcacg attccgatgg ttatcagcca cttcgccatg gtcttcaggg ggtaggccgg
    13861 cgtccgccgg ttgtcatgat atacccggcg gggcggcgtt tcgcgcccat ctgcgggcca
    13921 ttggcaacgt gctatgctcg catcccacag gcaagggagg ggaagcaatg ccactcatcg
    13981 caatgacccg cgagatgggg tcgctcggca aggacgttgc ggcggcgctc gccgaccgcc
    14041 tcggcaagca ggtggtgcat cacgagatca tcgacaatct cgccagcaag atgcgcctga
    14101 ggaagagcca cgtcatccgc ttcctcgagg gaaagagcgg gatctgggag cgcctgacga
    14161 cggacaagac cagcctctcc atctacaccg ccgacgaaac tttcgcactc gccgagagcg
    14221 gcaacgtcgc ggtgatccgg ggctggggcg ccacccacct gctgcgcccg gtgcagcacg
    14281 tgatctcggt ccgcgtgtgc gcgccgttcg aggtgcgcgt caagcgcatg ctggagcggc
    14341 tcaacaccga cgaccgcgat ttcgtggaaa acgagatccg gctctccgag gaggcgcacg
    14401 gcgcaatcac gcgccgccac ttcggcatca actggcagga ttccgagcat tacgacctcg
    14461 tgctcaacac cgagcgcctg acggtggagg aatgcgcgga cgaggtgacg gccctgctcg
    14521 acgactcgac gttccaggaa acggcgcaat cgcagcgcta cttccgcaat ctcgcgctgg
    14581 ccgctcatgt ccgggcggcg ctgcgccagg acgtccgcac cagcaagatg atgttctcga
    14641 tcacctgtga tgacggtgtc gccaccctcg cggggctggt ggaccccggc caggaaccca
    14701 aagacgcggt cgaggttgcc tcgaaggtgc ccggcgtcaa ggaaatcaga aaccagctgc
    14761 gcgtcgtcac cgccggcgcg cgccaccacg tcgaaggcta gcgcgcccgg gcacgcgaag
    14821 ggccgcgcgg tccgcggccc ttcgccccct cacgccccgc cgctcatgga taaggcgcca
    14881 tcggcttgcc ggtggtcagc aggtggtgca gcgcatacac ccagccgcgc atgcggtgat
    14941 agttccggta gtggctgtac tgctgcatcc tgatggtgtc ggcgagctcg tcgcggttct
    15001 tgccctccgc gatgccggcc ctaaccgctt tcaggaagtc ctcgtgcatc tgcgcgtact
    15061 cgagcaggtc ctcccggacc gacacgtccc cgtgtccacc gaggaacgtg tccgcgtcga
    15121 atgcggcggc ctttctcagg agcgcgatcc agccctcgac cgacggggta aaagtgtagt
    15181 tcgaccagtt gcggcgcgcg aacggcccgc cgaggaacag cgtccgggcg tgcgggaagt
    15241 ggatgagcgt gtcgcccgga ttctgccccg gcccgaggtg gatgagctcc gccgtgcgcc
    15301 cgcccagatg gatggtgaag cgcgaatcga aagtgacctc aggcagcacc atctgcagct
    15361 cgcccgggtc gaggtcgtag cgcttgaagt aggcctgccg gcgttgcacc tcatccttgt
    15421 agtacttctg catcatgccg cgcgtgtccc ggtgcgagac gatggtcgcg ccttcggatt
    15481 tgaacacggg attgccgagg aagtggtcgc cgtgcgggtg cgtcaccacc acccacttga
    15541 tcggctggtc ggtgatcttg cggatcattc cgaggagcat gcgcgcatcc gccgggtgct
    15601 gctgcgtgtc gacgaccatc accccttcat cggtgaccat gaacaccgcg ttcgacgatc
    15661 cgtcgttcca gaagaaatac aggtcgggcg cgatcctctt cagctgcgcc ggctgcggct
    15721 tcgcgccccc cttgagcgcc tccggcgcga gcttgccgag ctccgacgca tattgcgcgt
    15781 gggccccgcc cgtagcgagc agcgtcgccg cgcagatcag cgccgccgca actggtttcg
    15841 actgcctcat attggtctcc cccttggcac gccttgcgtc gcgtgcgtca aacctgcgga
    15901 ttgatccgct ccagcttcga gtgcagcttg ttgagcgcgt tgatgtaggc ctttgccgag
    15961 gccacgacga tatcggtatc ggcgccctgg ccgttgacga tgcgcccgcc cttcgtgagc
    16021 cgcaccgtca cctcgccctg ggcatcggtg ccggtcgtga tggcgttcac cgagtagagg
    16081 agcagctcgg agccgctgtt gacgatgctc tcgatcgcct tgaaggaggc gtccaccggt
    16141 ccgctgccct gcgcctgtgc ccggcgctcc ctgccgtctt ccgcgaccac gatcttcgcg
    16201 tagggcgcct cgccggtttc ggagtgggcg gtcagcgaaa ccagcttgta gtgctcgttc
    16261 tcgacgtagt cgctcgcctg ctcgctgatc agcgcctgaa ggtcctcgtc gaagatctcg
    16321 tgcttcttgt cggccaggtc cttgaagcgc ttgaacgcgg cgttcaacgc ctcctccgag
    16381 tcgagcatga tgccgatttc ctgcaggcgc tggcggaatg cgttgcgccc cgaatgcttg
    16441 cccagcacca ggcggttcgc gttccagccc acgtcctcgg cgcgcatgat ctcgtaggtg
    16501 tcgcggctct tgagcacgcc gtcctggtgg atgcccgctt cgtgcgcgaa agcgttcgcg
    16561 ccgacgatcg ccttgttggg ctgcaccggg aagccggtga tgcccgagac cagccggctc
    16621 gccgggacga tctgcgtcgt atcgatgttg gtgtcgcacg ggaagatgtc ctggcgcgtg
    16681 cgcaccgcca tgacgatctc ctccagcgcg gcgttgccgg cgcgctcgcc aagaccgttg
    16741 atcgtgcatt cgacctggcg cgcgccgttc aggaccgcgg cgagcgagtt cgcgaccgcg
    16801 agcccgaggt cgttgtggca gtgcaccgac cacaccactt tgtcggaatc gggaatgcgc
    16861 tcgcggagca gccggatcag ccgcccgaag tgctcgggga aggtatagcc caccgtgtcc
    16921 gggatattga tggtggtggc gcccgcccgg atcacccgct ccaggatgcg gcacaggaag
    16981 tcgatgtccg agcggcccgc gtcctctggc gagaactcga cgttgtccgt gtactggcgc
    17041 gcccacttca ccgccttgac cgcctgctcg atcacctggg agggctccat gcggagtttc
    17101 ttctccatgt ggatgggcga agtcgcgatg aatgtatgca ggcgcgggct ctttgccggc
    17161 ttgaccgcct cgccgcagcg ccggatgtcg ttctcgttgg cgcgggcaag cccgcacacc
    17221 gtgctgtcct tgatgatgga agcgaccgca cgcaccgcgt cgaagtcgcc gtcgctcgcc
    17281 gccgggaagc cggcctcgat cacgtcaacg tgcaggcgct caagctggcg cgcgatgcgc
    17341 agcttctcct ctttcgtcat ggatgcgccg gggctttgct cgccgtcgcg catcgtcgtg
    17401 tcgaatatga tcaatttttc cttggccatg gtgcctccgg gattgccgct gcccccggcc
    17461 cggcttgggc cggtgcagcg cgtgtttcat gagtttaccc gccgcggctt gtgccgcgcc
    17521 ggaccaaaac aagagtatgt gggaaaagag tatgcgcgcc ttaggggcgc agcagcaggc
    17581 ccgccagcag aaggccggcg agaagggatg gcgattgggc ctgcgtcagc cgtaacatgg
    17641 cacgaaaata tatcggtttt ccccccacga agcaaggggc gccggagcgc gacgcaggtg
    17701 cccgccttgc gggtgcgacg gcgcaggcgt ccgaacggcc aatgcgactt gatcggttcc
    17761 cgtcatgacc gatccgaggc cgccgtcgca acgttcagca gcggccgcgg ctcggttcaa
    17821 ctggcgaccg gcgggggtgg cgtgtggccc ggcccctttc ccttgaccat ctcgtagacc
    17881 cagatcacgt agcccgagca gctgtaaccc gcgaacagga gaaacagcat ctccggcagc
    17941 gtgctggaga gcatgaacag cgccaccagc ccgaaggcga tgccgaccac taccgagaac
    18001 ggcacgctct tccggaggtt gatgtccttg ccgctgtaga acttgaagtt gctcaccatg
    18061 gtgagcccgg cgaatacggt cagcccccag gcgagccact tcacgtcagc gcccttgatc
    18121 tggtactcgt tcaacgccca caccagcccc gcgatcagac acgccgcggc gggactcggc
    18181 aggccctgga aaaagcgctt gtcggcgacg tcgagcgtgg tattgaagcg cgcgagccgc
    18241 agcgccgcgc acacgcagaa gatgaaggcg gcgatccagc cgagcttggt ggtgagccac
    18301 ggtcccagcc acgccacggt cttcatgccg gagaaatcct tgagcgccca cacgtacacg
    18361 accagcgccg gcgccacgcc gaacgacacc atgtcggaaa ggctgtcgta ctcggcgccg
    18421 aacgcgctct gggtgcgcgt gagacgggcg atccgcccgt cgaggccgtc gaataccatg
    18481 gcgatgaaaa tcgccatcgc ggcctgctcg aagcgtccgc ccatcgcctg cacgatggcg
    18541 tagaagccgg cgaacagcgc gatcgtcgtg aacaggttcg gcagccagta aatgccgcgc
    18601 cgcgcaaagc gcgactggat gaaaagcttg tgctcgcgcg gttcgtacat agatccagta
    18661 agcggtcagc ggtaaacggt aagcggtggc gcggctgtcc ggcggacgtt caggaacccc
    18721 tgctcactgc tcacggctta ctgcttacgg aagcatcgca aggatgcttt ccgtggcgta
    18781 gaccttgtcg cccatcgacg ccaccggccg cgattcgggc ggcaggtaga catccacgcg
    18841 cgagccgaaa cggatgaagc cgtagcgttc gccgcgcgtg aggcgctgcc cggtccgggc
    18901 atagcacagg atgcggcgcg cgatgagccc cgcaatctgg acgcaggtga catccgcccc
    18961 gcccgcggtc cggatccaca gcgcgttgcg ctcgttctcc agcgacgact tggacagctc
    19021 ggcgttgaag aacttgcccg gatggtgcca cgcctcgcgg acctcgccgt ccacgggact
    19081 gcggttggaa tggacgttga acacgttcat gaacacgctc atcttgagcg cgtcgcgctc
    19141 gagataggga tcgcgcgcgc gctcgaccgt gacgatccgg ccgtcggccg gcgacagcac
    19201 cgccttcgac tcctgcggga tcgcgcgcgg gggatcccgg aaaaactgca ccaggaaagc
    19261 aacaccgagc cacagcggcg ccgcccacca ggcgccggcg agccagtgaa tgagcagtgc
    19321 ggccacgatc gacgccgcga tgaacggcca accctcccgc gcaatgatcg ggtgcgggta
    19381 gtcgcccacc ccgtgaggag tgaggggtga ggcgtgaggc gcggctccgc tcagatttga
    19441 ctcttcactc ttcactcctc actcctcact agttcttggt ctggtccacc agccggttct
    19501 tcttgatcca cggcatcatg tcgcggagcc gcgcgccgac gatctcgacc ggcaggctcg
    19561 ccgccatgcg tcgcatcgcc ttgagcgagg gcgcgccggc gcggttctcg aggatgaatt
    19621 ccttggcgaa ctggccggtc tggatctcga ggaggatctt cttcatctcg gcgcgcactg
    19681 catcgttgat gacgcggggc ccgcgcgtga agtcgccgta ctcggcggtg ttcgacaccg
    19741 agtagcgcat gttggcgatg ccgccttcgt agatgagatc gacgatcagt ttcacctcgt
    19801 gcaggcattc gaagtacgcc atttcggggg catagccggc ctccaccagc gtgtcgtagc
    19861 cggcctggat cagcgccgtc aggcccccgc acaacaccac ctgctcgccg aagagatcgg
    19921 tctcgcattc ctcgcggaag gtggtttcga tcacgcccgc gcgtgccgcg ccgatcgccg
    19981 cggcgtagga gagcgcgacg tcgcgcgcat tccggctcgg gtcctggtac accgcgatca
    20041 gcgatggcac gccgccgccc tgggtatagg tcgagcgcac cagatggccg ggccccttgg
    20101 gcgcgatcat gatgacatcg aggtcggcgc gcggctcgat ctgcttgaag tggatgttga
    20161 agccgtgggc gaaggcgagc gtcgagcctt tcttgatgtt gggctcgatc tcgtccgaat
    20221 aaacctgtgc gtgatgctcg tccggcagca ggatcatcac gatgtcggcg ctttttaccg
    20281 cgtcgcccac cggcttcacc ggcagaccgg cgttcttcgc cttggtccag gacgcgccgc
    20341 ccttgcgcag gcccacggtc accctcacgc cggaatcctt caggttgttc gcgtgcgcgt
    20401 gaccctgcga gccgtagccg atgatggcca cgtgcttgtg cttgatcagc gacaggtcgg
    20461 cgtctttgtc gtagtagatc ttcatgagtt tccctttagt caacagctct ctgcaatcag
    20521 ccgtcagcta ccagcattca gtttacggca cgatggtttg tgctgatcgc tgaatgccga
    20581 gtgctgaacg ctaaactctc aacacccggt cgccgcggcc gatgccggag gcgccggtgc
    20641 gcaccgtctc caggatcgcg gcccggtcca gcgccttgag gaaggcgtcg agcttggagc
    20701 cggtgcccgt cagctcgatg gtatagtccc ggtcggtgac gtcgatgatg cggccgcgga
    20761 agatgtcggc catccgtttc atttcctccc ggtccttgcc caccgcgcgc accttgacca
    20821 gcatcagctc gcgctcgatg tggtcgccgt cggaaagatc gatcaccttc accacgtcga
    20881 tcagcttgtt cagctgcttg gtgatctgct cgatcacctc gtcggagccg gtggtgacga
    20941 tggtcatgcg cgatagcgtc gcgtcctcgg tcggcgcgac ggtgagcgac tcgatgttgt
    21001 agccgcgcgc ggagaacagc ccggccacgc gcgacagggc tccggcctcg ttctcgagca
    21061 ggatggagac gatgtgcctc ataccaggat catttccgag agacccttgc cgcccggcac
    21121 catcgggaac acgttctcgg tctggtcggt gatgaagtcc atgaatacca ggtcctgctt
    21181 gcgcgcgaac gcctccttca gcgcgggctc cacgtcctcc ggtttctcga tcttcatgcc
    21241 gacgtgcccg taggcctcgg cgagcctgac gaagtccggc agcgcgtcca tgtacgactc
    21301 ggcgtagcgg ttgccgtgga agaactcctg ccactgccgc accatgccca tgtacttgtt
    21361 gttcaggttc accaccttga tcggcagccg gtactgcttg caggtggaga gctcctggat
    21421 gcacatctgc acgctcgcct ccccggtgac gcaggcgacc gtttccttcg ggtgcgcgag
    21481 ctgcacgccc atcgcggcgg gcaacccgaa gcccatcgtc ccgaggcccc cggaattgat
    21541 ccagcgccgc ggcttctcga accggtagaa ctgcgccgcc cacatctggt gctggccgac
    21601 gtcggaggtg atgaaggcgt cgccgcgggt cacctcgtat agtttctcga tcacgtactg
    21661 cggcttgatg agcgggctcg agcggtcgta cttgaggcag tcgcgcgagc gccacagctc
    21721 gatctgcgtc caccaggtct tgagcgcttc ctggtccggc ccgtccttcg aggcctcgag
    21781 ctggcggagg aactcgcgca gcacgtcgcg tacgttgccg acgatcggga tgtcgacttt
    21841 cacgcgcttc gagatcgagg aagggtcgat gtcgacgtgg acgatggtgc ggggttcggc
    21901 gaagaagtgc ctgggattgc cgatcacccg gtcgtcgaag cgcgcgccga tcgcgagcag
    21961 cacgtcgcag tgctgcatcg ccatgttggc ttcgtaggtg ccgtgcatgc cgagcatgcc
    22021 gaggaactgg cggtcggtcg ccgggtacgc gccgaggccc atcagggtgt tggtgcaggg
    22081 aaagccgagc agccgcacga gctgcgccag ctcttccgag gccccggaga ggatcacgcc
    22141 gccgccggtg tagatcatcg ggcgcttggc cttcatcagc gcctggatcg ccttcttgat
    22201 ctggccggcg tgtcccctca ccaccggatt gtaggagcgc atggtgatcg attcggggta
    22261 gtgga
//
LOCUS       DBNGWZ010000524        13797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_697206, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000524 DBNGWZ010000000
VERSION     DBNGWZ010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_697206"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..192
                     /locus_tag="AC16CX_16100"
     CDS             55..192
                     /locus_tag="AC16CX_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3014067.1"
                     /translation="MEIKTGVAAPDFTLAAHNTDQKIALAGFRGRPVVVAFMPFAFTG
                     G"
     gene            205..516
                     /locus_tag="AC16CX_16105"
                     /pseudo
     CDS             205..516
                     /locus_tag="AC16CX_16105"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            727..1956
                     /locus_tag="AC16CX_16110"
     CDS             727..1956
                     /locus_tag="AC16CX_16110"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virginiamycin B lyase family protein"
                     /protein_id="HIR3014068.1"
                     /translation="MKPLISALTAALLCSIPPAYGQQKPPNLPDGPGKEAVVTYCSGC
                     HGLARVANSGYPQAYWHTSIRMMLNFGVPIPPDQVIPVTDYLAKNFPEKPKPAANIIH
                     GPARIDIKEWQVPIPGSRPHDPLATRDGAIWYTGQMANRLGRVDPKTGQVREYPLKSP
                     LTAPHGLVEDKDGNIWFTGNHISIIGKLDPRTGAVTEYKMPDPKAKDPHSLAFDQSGI
                     LWFTLQQSNMMGRLDPGTGEIKLLTAPTPRSRPYGIMVNSKGVPFVALFGTNKIASID
                     PGTLEIREYALPDPKARPRRLALTGDDVVWYSDFSRGYLGRLDPATGQVTEWQSPGGP
                     RSEPYGIAVAKDVIWYSESGTRPNTVVRFDPKTRQFQTWAIPSGGYIVRKMDVTPDGN
                     PVLATSTVNGVALVEVK"
     gene            2024..2686
                     /locus_tag="AC16CX_16115"
     CDS             2024..2686
                     /locus_tag="AC16CX_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014069.1"
                     /translation="MKGAGFGRIAAVALLSLGFAAGAQTQSSDMTFFITSVGLGKGAD
                     LDGLAGADKHCQSLAAAAGAGKRMWRAYLSTTASGGSPAVNARDRIGKGPWHNFEGRL
                     IARNIQELHEGVNNINRMTALTEKGTHVNGRADAPNMHDILTGTQPDGTAFPAGKDTT
                     CGNWTKSGEGIAIVGHHDRWGLREDAPSVSWNSSHPSRGCSQDNLRASGGAGLLYCFA
                     AK"
     gene            complement(2783..3754)
                     /locus_tag="AC16CX_16120"
     CDS             complement(2783..3754)
                     /locus_tag="AC16CX_16120"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014070.1"
                     /translation="MRTLKAPLCLALLALLACPLPAAAQKYPDKSIRLVVAFPTGAPY
                     ILALLLSEKLRESLGQPVVPDFRAGAGGNLAAEVVAKAPADGYMLLLTSGSIAISPSL
                     FPKLGYDTFRDFAPVTLLATVPNVLVVHPSVPARNLSEFVKLAKAHPGKLNYGSGGVG
                     SGSQLGSELFKTLAGIRMVHVPYKGAALALSAMLSGEVDMVTSTVPATIPLVNSGRIR
                     ALAVMAPARVASLPNVPTTAEAGMPEVVVITWYGLFVPAGVKPDIIERLNGEIVRLMN
                     APETRSKLAQVELEASTSTPAEAGKFVRAEMERWARVIKEAGIRVQQ"
     gene            complement(3845..4549)
                     /locus_tag="AC16CX_16125"
     CDS             complement(3845..4549)
                     /locus_tag="AC16CX_16125"
                     /inference="COORDINATES: protein motif:HMM:NF020475.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR3014071.1"
                     /translation="MKYEDPACAYCPPTVRACRQGEEAERGPGFCPTKVDPATQAAAL
                     ARYEDPEIRRISRESALVESEGYCKWTRVEEIVQFARRMGYRKIGIANCISFVDQAYV
                     LSGILESHGFEVVSASCKTGSIPKEDLGIADHQKVRPGQFEALCNPVCQAELLNEHGC
                     ELNIVLGLCVGHDSLFFKHVKGLTTVLVAKDRVLGHNPVAALQLADTYYSRVWGPLKP
                     AKPPKLPVAGRRGAKR"
     gene            4933..5139
                     /locus_tag="AC16CX_16130"
     CDS             4933..5139
                     /locus_tag="AC16CX_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014072.1"
                     /translation="MRPTRSVDWTRDRIAALSTPDVRQLRVNAERLNAPEVMKLCDAV
                     LGERPRGLARQPRKKKAPEPGGTA"
     gene            5136..5489
                     /locus_tag="AC16CX_16135"
     CDS             5136..5489
                     /locus_tag="AC16CX_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014073.1"
                     /translation="MSIPVADRKQFMVGKYEVIAEPVPHSAHMLRYTVYLDKTRIGAM
                     VSVPTESDCRFLEKPPVVPPLKIFYVTSRPGRPKKDAKPAVALERDQPGFREKLPSDV
                     PLDGVNRENGVNRES"
     gene            5777..6724
                     /locus_tag="AC16CX_16140"
     CDS             5777..6724
                     /locus_tag="AC16CX_16140"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3014074.1"
                     /translation="MTGAVRNPDLERNKAVALRFKKSHGTREMPQVEKEVLAPNYNRI
                     RGGNFHLASNARGEGWPEPGRYLRAAFPDRVDIIEHVVAEGDTVGLLFRLTATHTGSL
                     FGIPPTGRKVDVYEIALLRIVNGRMVEGWFMMDEAELLRQLDARMPARKDGRIIAPPV
                     PDLGESGDALEQRLSSKKPLSPLDQHKVILARSRSSAPSTANRSPDFARKREAFQHLH
                     DRGVARGVGGQTLRSALTDRQDRIDRLVAEGDQAWMRFYIYGTHQASFYCLPATGKRV
                     GVPAVDVSRFAGGKWKESWCFGDELGLLLQLGASDPLFE"
     gene            6777..7067
                     /locus_tag="AC16CX_16145"
     CDS             6777..7067
                     /locus_tag="AC16CX_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3014075.1"
                     /translation="MAKGYWINMFRSIKDPAKVEAYRKLAAPVMQASGGRFLVRGDPT
                     KVYEHGAMQRVVVIEFDSVEKAIAVHDSPGYQAALKALGDGAVRDLRIVEGL"
     gene            7253..7693
                     /locus_tag="AC16CX_16150"
     CDS             7253..7693
                     /locus_tag="AC16CX_16150"
                     /inference="COORDINATES: protein motif:HMM:NF014168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR3014076.1"
                     /translation="MGQANTPVTRDEVILLAGRLALGAIFVKSGFQKLLALGAFAASL
                     AQRGVPQSGFWAVVGATVEFVGGILIVTGLRTREASLLMILFVIVATGISHRYWEFAD
                     AAARRAQESQFFKNLSIIGGFLLLFVTGGGRFSLDALMRRRKDG"
     gene            complement(7840..8811)
                     /locus_tag="AC16CX_16155"
     CDS             complement(7840..8811)
                     /locus_tag="AC16CX_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014077.1"
                     /translation="MTTSRSWSIAGAALAVVFAATAQPQDYPAKPIRVVVPFPPGGGT
                     DLMARTVIQKLGESLGATVVVENRGGAGGSIGTDLVAKSPPDGYTLLVVSGSHAINPS
                     LYPKLPYDSVRDFAPVTMLTSGPGLLVVHPSLPARTVKEFIALARSRPGQLHYASAGI
                     GTPPHLAGELFKTMTGADIVHVPYKGNGPAYTDLIGGHVSVMFPTIPTAIPHVRTGKL
                     RALGVTTRQRTAIAPELPTLSESGVPGYDVSSWYGLLAPAGTPAAVVARLQREIAKVL
                     RLPDVSEKLMAQGLDLVGNTPEEFAGIIKSEIAKWAKVVKASGAKAD"
     gene            complement(8836..9918)
                     /locus_tag="AC16CX_16160"
     CDS             complement(8836..9918)
                     /locus_tag="AC16CX_16160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012407.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3014078.1"
                     /translation="MAGRHEIELAVLEGDGIGPEITDATVRILKAAARDAGLGVRLKP
                     CLFGWRSYKKHRTTLCDATLRALGRHEGWIVGPTAAGEYPKDDPYRGHPNGFIRRHFK
                     LFANVRPVQSWPQLNPLIPDLDITILRENTEGFYPDRNLAWGYGEFKPRGDVALSLRV
                     ITGEACDRFARYAFQYAAAAGEKRIAVVHKRTALPQTDGLFVGAFERLRSRYRDIAIE
                     PVRIDTFSSSFPREPHRYRLVATTNLFGDILSDQACGLAGGVGLAPSLNAGLEHAMAQ
                     AVHGTAPDIAGQGKANPSALILSTALLLRWFYLRTKSRACRDAAALMEDGVKSAIASG
                     ATTPDLGGRATTAAFARAVIDAMRDE"
     gene            10035..10997
                     /locus_tag="AC16CX_16165"
     CDS             10035..10997
                     /locus_tag="AC16CX_16165"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014079.1"
                     /translation="MKPVTLLLVLAGSLLLPGASAAQPYPARTVRLVVGFTAGGATDI
                     SARALAQKLSGPLGQQVVVENRTGAASTLASEFVARSAPDGYTLLLANATVAMPSLFA
                     RLGFDVRKDLAPVSLVGYGPLVLVAHPSLPVKSVKELTALARRKPGMLNYGTAGVGSM
                     THLAMALFVSMTSVDMVHVPYKGSSQASIGAITGEAQLMFSSPAAVMAQLQQGRLRAI
                     AVSGTRRSAVLPDVPTIAEAGLAGYDATSWYGMLAPAATPRPAISRLGDESVKALAAS
                     DLRERLLALGIEPAKGGVDEFAGYLEAEIAKWAKVIKAAGIPPQ"
     gene            11222..12133
                     /locus_tag="AC16CX_16170"
     CDS             11222..12133
                     /locus_tag="AC16CX_16170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014080.1"
                     /translation="MNAPLKKVKASMMNAIHDLPLLVARDEGYFRDEGLDVEIVRTPG
                     SGQRDSDHRALRANIFERTMEALYDQGACDQFRMCEWGVMKRAVESTVAGGHRPAKIV
                     ALGSAMSSFAIMTDPKQRIYEPEQLKDRPVAVSPFNGSHFTTLKLLEGFLKREQIKTA
                     FAGTMKERMEAVARGEVAAASLMEPWISIAEKRGLRVLMESHSTRSEAAGDELDGPTL
                     AKMFRAEAAAAVAIEKNPGRYAHYFVEEAGGLLEPGDLKLSRILNAPPQPYTRDRFEH
                     TYQWTLGWGLVPPGATYENTVDNRAWQ"
     gene            complement(12135..12761)
                     /locus_tag="AC16CX_16175"
     CDS             complement(12135..12761)
                     /locus_tag="AC16CX_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3014081.1"
                     /translation="MTVSEAHRITDVDTLEKLYGVASGAAVEKEISYLHPHYQRLIAA
                     SPFFILATGGPGGMDASPRGDAPGFVVVEDEKTLLIPDRRGNNRVDSLRNIIADPRVA
                     LLFLIPGIGETLRVNGRAEIRTDPALLARFRVEGKLPRSVLVVRVDTVFFQCARAIFR
                     AKLWDPARHLERGSLPSLGTIISDLTHARFDGKEYDEGLYERLKEALY"
     gene            12925..13155
                     /locus_tag="AC16CX_16180"
     CDS             12925..13155
                     /locus_tag="AC16CX_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014082.1"
                     /translation="MTKGVSRMPLLALSSRPWIGFRKLFLGVLGGEDFLFLCGSAFLA
                     VRLFLSFTSLAAFAVKLRARPLRGKLITHHPV"
     gene            13202..>13797
                     /locus_tag="AC16CX_16185"
     CDS             13202..>13797
                     /locus_tag="AC16CX_16185"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3014083.1"
                     /translation="MPPSNDLPDILETTEAERLATGFTFTEGPLWHPDGYWYFVDIRQ
                     NKLYRMTPGRAPEVFKETSGGNGTTFDLQGRLILCEGTARRVVRIGHDGKVEALVDRY
                     KGGRFNRPNDVVCRSDGSIYFTDPDKRVPYAEREIPGPAGIDNLWDGASVYRVATDGA
                     LSVLALCEYPNGLAFSPDERTLYVANTRSSKYIHALEL"
BASE COUNT         2442 a         4647 c         4412 g         2296 t
ORIGIN      
        1 cgcgggtatt cttcgctggc ggctctgccg accccactac aaggagaacc ccgcatggaa
       61 atcaaaacag gcgtcgcagc gcccgatttc acgctcgccg cgcacaacac cgatcaaaaa
      121 atcgcgctcg ccggtttccg cggccggccg gtcgtggtcg ccttcatgcc gtttgcgttc
      181 accggcgggt gaaccaacca ggtcaacggg ttccgtgcga actacgaaca attcaagaag
      241 ctgaacgtcg agatcctcaa catcagcgcc gaccccgttc ccagcctcaa ggcctgggcc
      301 gaaaaactag gcggcgttcc cttccccctg ctctcggatt actggccgca cggcgcggtc
      361 ggcaaggcct acggcatctt caatgaggag cgcggcaggg acaagcgcgc ggcgtacgtg
      421 cttgacgcca agggaatcgt ccgctacgcc aagctttacg accccggtac catccccgaa
      481 agcaaggaac tgctcgcgga gctgcgcaag ctctgatatg agcttccggg tgataggtga
      541 taagtgatag gagccgagac aaaaagcgtg ataggtgata ggtgacagga gtaaattccc
      601 gcgcgagcgc gggtgactta tcacttatca cgccgttcga gtcttctcac ttgtcactta
      661 tcacgccttc gcgcggcatc gcccgcatcc cgtcactcat gatttcgtga aacagggact
      721 ccatccatga aaccgctgat ttccgcactg accgccgcac tgctttgctc gatcccgccc
      781 gcctacggcc agcagaaacc gccgaacctg cccgacgggc ccggcaagga agccgtcgtg
      841 acctattgca gcggctgcca cgggctcgcc cgcgttgcga attcaggcta cccgcaggcg
      901 tattggcaca cctccatccg gatgatgctc aatttcgggg tccccattcc gccggatcag
      961 gtcatccccg tcaccgacta tctggcgaag aactttcccg agaagccgaa gccggccgcc
     1021 aacatcatcc acggcccggc aaggatcgac atcaaggagt ggcaggtgcc gataccgggc
     1081 tcgcggccgc acgacccgct cgccacccgg gacggcgcga tctggtacac gggacagatg
     1141 gccaaccggc tcgggcgcgt cgatccgaag accggccagg tcagggagta tccgctcaag
     1201 tcgccgctca ccgcgccgca cgggctggtc gaggacaagg acggcaacat ctggttcacg
     1261 gggaaccaca tatcgatcat cggcaagctc gatccgagga cgggcgccgt caccgaatac
     1321 aagatgccgg acccgaaggc gaaggatccg cactcgctcg ccttcgatca gagcggcatc
     1381 ctgtggttca cgctgcagca atccaacatg atgggacggc tcgatccggg caccggcgag
     1441 atcaagctcc tgaccgcgcc gacgccgcgc tcgcgcccct acggcatcat ggtgaattcg
     1501 aagggcgttc ccttcgtcgc cctgttcgga accaacaaga tcgcgagcat cgatcccggg
     1561 acgctggaga tccgcgaata cgcgttgccg gacccgaagg cgcgcccgcg gcgcctggcc
     1621 ctcaccggcg acgacgtcgt ctggtactcg gacttctcac gcggctatct gggccgcctc
     1681 gaccccgcga ccggacaggt taccgagtgg caatcgcccg gcgggccgag atcggagccc
     1741 tacggcatcg ccgtcgcgaa ggacgtcatc tggtacagcg aatccgggac caggccgaac
     1801 acggtcgtgc gcttcgaccc gaagacgcgg cagttccaga cctgggcgat cccgtccggc
     1861 ggctacatcg tgcgcaagat ggacgtcacg cccgacggca acccggtgct ggccaccagt
     1921 acggtgaacg gcgtcgccct cgtcgaggtg aaatgatctc caggaatacc gcgaggcgcg
     1981 ccgttgttta caggatcgca cgaccatccc atggaggtgt gaaatgaaag gagcagggtt
     2041 cggcaggatc gcggcggtcg cgctgctgtc gctgggcttc gccgccggcg cgcagacgca
     2101 gtcgtccgac atgacgttct tcatcaccag cgtcggcctc ggaaagggcg cggacctgga
     2161 cggcctggcg ggcgccgaca agcactgcca gtcgctcgcg gccgcggcgg gcgcgggcaa
     2221 gcgcatgtgg cgcgcctacc tcagcacgac cgcgtccggc ggctcgccgg ccgtgaacgc
     2281 gcgcgaccgc atcggcaaag ggccgtggca caacttcgaa ggcaggctca tcgcgcgaaa
     2341 catccaggaa cttcacgaag gcgtcaacaa catcaaccgc atgaccgcct tgaccgaaaa
     2401 gggaacccac gtcaatggcc gcgccgacgc gcccaacatg catgacatcc tcaccggcac
     2461 gcagccggat gggacggcgt tccccgccgg caaggatacg acctgcggca actggacgaa
     2521 gagcggcgag ggcatcgcga tcgtcggcca ccacgatcgg tggggcctgc gcgaagacgc
     2581 gccttccgtg tcctggaact cctcgcatcc ctcgcgcggc tgcagccagg acaacttgcg
     2641 cgcttcgggt ggcgcgggtc tgctgtactg cttcgccgcc aaatgacggg atgcgattcg
     2701 tgagctgaac cggcccgctt caacgcgctc gccttatcga cctcgccgga tcagtgacgg
     2761 cccccgctgg gccgggccac ccctattgct ggacgcggat accggcctcc ttgatcacgc
     2821 gcgcccagcg ttccatctcg gcacgcacga actttcccgc ttcggccggc gtgctggtgg
     2881 acgcctcgag ctccacctgg gcgagcttgc tccgcgtttc gggcgcgttc atcagtctga
     2941 cgatctcgcc gttcaggcgc tcgatgatgt cgggcttcac gcccgcgggc acgaacaagc
     3001 cgtaccaggt gatcacgacc acctccggca tgccggcttc cgcggtcgtc ggcacattgg
     3061 gcagcgacgc cacgcgcgcc ggggccatca ccgccagcgc ccggatcctg ccgctattga
     3121 cgagcgggat ggtcgcggga acagtcgagg tcaccatgtc cacttcgccg ctcaacatcg
     3181 cggacaacgc cagcgcggcg cccttgtagg gcacgtgcac cattcggatt ccggccagtg
     3241 tcttgaacag ctcgctcccg agctggctgc ccgagccgac gccgccggag ccgtagttga
     3301 gcttgcccgg atgcgctttg gccagcttga cgaactcgct gaggttgcgc gccggcaccg
     3361 aagggtggac gacgagcacg ttgggcacgg tcgcgagcag cgtgaccggc gcgaagtcgc
     3421 ggaacgtatc gtagcccagc ttcgggaaca ggctggggct gatggcgatc gagccgctcg
     3481 tcagcaacag catatagccg tccgcaggcg ccttcgcgac cacctcggcg gcgaggttgc
     3541 cgcccgcccc cgccctgaaa tccggcacga ccggctgccc gagcgactcg cgcagcttct
     3601 ccgagaggag gagcgccagg atatacgggg caccggtcgg gaacgccacc accagccgga
     3661 tgctcttgtc ggggtacttc tgcgccgcgg cgggcaacgg gcatgccagc agggccagca
     3721 gggcaaggca aagcggtgct ttcaaagtgc gcatgttctc tcctccgggc gcttcgtcac
     3781 tttgagaccc cgacatgaat cacttatcac ctatcaccgg ttttagccag tcacgcttct
     3841 tcattcacct cttggcgccg cgccgtccgg ccacgggaag cttgggcggc ttcgccggct
     3901 tgagaggccc ccagacgcgg ctgtaatagg tatccgcgag ctgcaacgcc gccaccgggt
     3961 tgtggccgag cacgcgatcc ttcgcgacca ggaccgtcgt cagccccttc acgtgcttga
     4021 agaacaggct gtcgtggccg acgcacagcc ccagcacgat gttgagctcg cagccgtgct
     4081 cgttgagcag ctcggcctgg cacaccggat tgcagagcgc ctcgaattgc ccggggcgga
     4141 ctttctggtg gtcagcgatg cccaggtctt ccttcggaat gctgcccgtc ttgcaggagg
     4201 ccgacaccac ttcgaagccg tggctctcca ggattccgct caacacgtaa gcctggtcga
     4261 cgaagctgat gcagttggcg atgccgatct tgcgatagcc catgcgcctg gcgaattgca
     4321 cgatctcctc gacccgcgtc catttgcagt agccttcgga ttcgaccagc gccgactccc
     4381 gcgagatcct gcggatctcc gggtcctcgt agcgcgcgag cgcggcggcc tgcgttgcgg
     4441 ggtcgacctt ggtcggacag aagccggggc cgcgctccgc ctcctcgccc tggcggcagg
     4501 ccctgaccgt cggcgggcaa tacgcgcacg cgggatcttc gtatttcatc gggtattcct
     4561 ccactgcaac tgcgtcgatt caccatgcta gacgcccgcg cggaaggcga ctttgtccta
     4621 gatcaaaatg ggctcatgcc cctccgcccc tggatggccc cgggaacggc gtgacaagtg
     4681 acgagtgata agtgataagg attcccgcgc tgcgggagtt gcccctatca cctatcactt
     4741 atcacctatc accaattggc cagaactccg aaaggcggag cacggtcgaa ccggaccgaa
     4801 cgccagttcg atcgacgtga atcggcggga ctcggaagct tctgtggtaa gatatgcaac
     4861 cagaaagccc ggccattccg ggctttttcg tccgtattgc agccttcggg agacatatcc
     4921 atcatgggaa tcatgaggcc cacccggtcg gtggactgga ccagagacag aatcgccgcg
     4981 ctgtccacgc ccgacgtgcg gcagctacgc gttaacgcgg agcggctcaa tgcgccggaa
     5041 gttatgaaac tatgcgatgc ggtgctcggc gagcggccaa ggggcctcgc ccgccagccg
     5101 cgcaaaaaga aggctccgga gccaggcggg acggcatgag cattcctgtc gcggaccgca
     5161 agcagttcat ggtcgggaag tacgaggtga tcgcggagcc cgttccgcac tcagcgcaca
     5221 tgctgcgcta caccgtgtat ctcgacaaga cgcggatcgg cgccatggtg agcgtgccga
     5281 cggaatccga ctgccggttt ctggaaaagc cgccggtggt cccgccgctg aagattttct
     5341 atgtcacgag ccggcccggc cgcccgaaga aggacgccaa gcccgccgtc gcactggagc
     5401 gggaccagcc cggtttccgg gaaaagctcc cgtccgacgt gccgctcgac ggcgtaaatc
     5461 gagagaacgg cgtgaatcgc gaatcgtgat ttgatctttt agaaagcccc ctccttcaat
     5521 tatcaggggt ggcgcggcag cgccggggtc gttggatttt ctccttctgc cttccccctt
     5581 cttcctaccg ccttccgccg taactcctca tccctcatcc ctccgccctc atccctcaca
     5641 aatgggcttg cccatttgtc gtggccgggg tttggcgcga tacttcgaga aaaaacgtgt
     5701 cccatgtttt ttgtcacgaa caatcgcatc gcgctcgacc ggtctcaccc atcttgagca
     5761 agcctgaaaa ggaagcatga ccggcgccgt gaggaacccc gacctggagc gcaacaaggc
     5821 ggtcgcgctg cgcttcaaga agtcgcatgg caccagggag atgccgcagg tcgagaagga
     5881 agtcctggcg cccaactaca accggatacg cggcgggaac ttccacctgg cgagcaacgc
     5941 gcgcggcgag gggtggccgg agcccggcag gtatctgcgc gccgcgttcc ccgaccgcgt
     6001 ggacatcatc gagcacgtcg tcgccgaggg tgacacggtg ggcctgctgt tccgcctcac
     6061 cgccacgcac accggcagcc tcttcggcat cccacccacg ggcaggaagg tggacgtcta
     6121 cgagatcgcg ctgctgcgga tcgtgaacgg ccggatggtc gaaggctggt tcatgatgga
     6181 cgaagcggaa ttgctgcggc agctcgacgc gagaatgccg gcgcgcaagg acgggcgcat
     6241 catcgcgccg ccggtgccgg acctgggcga gagcggcgat gcgctcgagc agcggctatc
     6301 gtcgaagaag ccgctctccc cgctggacca gcacaaggtg atcctggccc gttcccggtc
     6361 atccgcgccc tccaccgcga accgcagccc ggatttcgcg cgcaagcgcg aagcgttcca
     6421 gcacctgcac gaccggggcg tcgctcgcgg cgtcggcggg cagaccctgc ggagcgcgct
     6481 gaccgaccgg caggaccgga tagaccgcct cgtcgccgag ggcgaccagg cctggatgcg
     6541 cttttacata tatggcaccc accaggcgag cttctactgc ctgccggcga ccggcaagcg
     6601 cgtgggcgtg cccgcggtgg acgtcagccg cttcgccggc ggcaagtgga aggaaagctg
     6661 gtgcttcggc gacgagctgg ggctgctgtt gcaactcggc gcgtccgacc cgctcttcga
     6721 gtaactgtca ctcatcactt agcacctatc acccatacct tcaaaggaga cccgtcatgg
     6781 ccaagggata ctggatcaac atgtttcgct ccatcaaaga cccggcaaag gtcgaggcgt
     6841 accgcaagct tgccgctccg gtcatgcagg catccggcgg gcgattcctg gtgcgcggcg
     6901 acccgacgaa ggtgtacgag cacggcgcca tgcagcgcgt ggtggtcatc gagttcgaca
     6961 gcgtggagaa agcgatcgcc gtgcacgaca gtccgggcta tcaggccgcg ctcaaggcgc
     7021 tcggtgacgg cgcggtgaga gatttgcgga tcgtcgaggg actatagaga aggatgaggg
     7081 gtgagccggg aattcgaaga acgtgcctgc gcacgttcga gccggcgaac gcccgacgcg
     7141 atgcagcgcg gcgtgccggg gtcactgatc aaggatgacc cccgcggtgc gggagactca
     7201 tcacccatca ctggttatac tttcatgaca attcgataag gaggggaccg gtatgggaca
     7261 agccaacacg cccgtcacgc gagacgaagt gatactgctg gcaggccgcc ttgcgctcgg
     7321 cgcgatcttc gtgaagagcg ggtttcagaa gctcctggcg ctgggcgcct tcgcggccag
     7381 cctcgcccag cgcggggttc cgcagtccgg gttctgggcg gtggtcggcg ccaccgtcga
     7441 gttcgtcggc gggatactca tcgtcaccgg gttgcgaacg cgcgaagcgt cgctgctgat
     7501 gatcctgttc gtgatcgtcg ccaccggcat ttcgcaccgc tactgggaat tcgcggatgc
     7561 cgcggcgcgg cgcgcgcagg aaagccagtt cttcaagaac ctctccatca tcggcggctt
     7621 tctgctgctg ttcgtgaccg gcggcggccg gttctcgctc gacgctttga tgcggcgtcg
     7681 caaggacggc tgagcgacac cgtgcaacaa gacagatgac aaaaaagcct tgccgccaag
     7741 gaggcggagg acgcccaata tcggttcacg gtccctcagt cttcggtcgt cggtcgtcac
     7801 tggggttacg gcccgcctcc cgcttccaga caatccgcgt caatccgcct tggcgcccga
     7861 ggccttcacc accttggccc atttcgcgat ctcggacttg atgatgccgg cgaactcctc
     7921 cggagtattg cccacgagat cgagaccctg ggccatcagt ttttcgctga cgtccggcaa
     7981 ccggagcacc ttcgcgatct cccgctgcag cctggcgacc accgcggcgg gcgtcccggc
     8041 gggagcgagc aacccgtacc acgagctcac gtcataccct ggcacgcccg attccgagag
     8101 ggtcggcagt tccggcgcaa tcgccgtgcg ctgacgcgtc gtcacgccga gcgcgcgcaa
     8161 tttgccggtc cgcacgtgcg ggatcgccgt gggaatggtc gggaacatca ccgacacgtg
     8221 cccgccgatg agatcggtgt acgccggccc gttgcccttg tacggaacgt gcacgatatc
     8281 cgcgccggtc atcgtcttga acagctcgcc cgccagatgg ggcggcgtgc cgattcccgc
     8341 ggacgcataa tgcagctggc cggggcggga cctggcgagg gcgatgaatt ccttcaccgt
     8401 cctggcaggg agcgacgggt gaaccaccag cagaccgggg cccgacgtga gcatcgtcac
     8461 cggcgcgaaa tcgcgcacgg aatcgtacgg aagcttcggg tacaggctcg gattgatggc
     8521 atggcttccg ctcaccacga gtagcgtgta gccgtcgggc ggcgacttgg ccacgagatc
     8581 cgtgccgatg cttccgccgg cgccgccccg gttctcgact acgacggtcg cgcccaggct
     8641 ctcgccaagc ttctggatga cggtccgcgc catcaggtca gtgccgccgc ccggcgggaa
     8701 cggcacaacg acgcggatcg gcttggctgg atagtcctgc ggctgggcgg ttgccgcgaa
     8761 aacgacggca agcgcagccc ccgcgatgct ccatgaacgg ctggtcgtca tggtctcctt
     8821 ttcactcgtc actcgtcact cgtcacgcat ggcgtcaatg acggcccgcg caaacgcagc
     8881 cgtcgtcgcc cggccgccca ggtcgggtgt cgtcgccccc gacgcgatcg cgctcttcac
     8941 gccgtcctcc atcagcgccg ccgcgtcgcg gcacgcccgg ctcttcgtcc gcaggtagaa
     9001 ccagcgcagc agcagcgcgg tggacaggat cagggcggac ggattggcct tgccctggcc
     9061 cgcgatgtcg ggcgccgtgc cgtgcacggc ctgcgccatc gcgtgctcga gacccgcgtt
     9121 cagcgacggc gcgagcccca ccccgcccgc cagcccgcag gcctggtcgg acaagatgtc
     9181 cccgaacagg ttcgtcgtcg ccaccagccg gtaacggtgc ggctcgcgcg gaaacgacga
     9241 gctgaaggtg tcgatgcgca ccggctcgat tgcgatgtcc cggtagcggc tccgcaaccg
     9301 ctcgaacgct ccgacgaaca gcccgtcggt ctggggcaag gccgtccgct tgtggacgac
     9361 ggcaatgcgc ttctctccgg cggccgccgc gtactggaac gcgtagcggg caaaccggtc
     9421 gcacgcctcg ccggtgatca cgcgcagcga cagcgccacg tcgccccgcg gcttgaattc
     9481 cccgtatccc cacgccaggt tgcgatccgg gtagaagccc tcggtgttct ccctcagtat
     9541 ggtgatgtcg agatcgggaa tcagcgggtt cagctgcggc caggattgca ccggccgcac
     9601 gttggcgaac aacttgaaat gccgccggat gaagccgttc ggatgcccgc ggtagggatc
     9661 gtccttcgga tattccccgg cggcggtggg acccacgatc cacccctcgt gccggccgag
     9721 cgcgcgcaac gtcgcgtcgc acagcgtcgt gcgatgcttt ttgtaggagc gccagccgaa
     9781 gagacacggc ttgagccgga ctccgagccc cgcgtccctg gccgcagcct tcagaatccg
     9841 gaccgtcgca tcggtgattt cgggaccgat gccgtccccc tccagcaccg ccaactcgat
     9901 ctcgtgcctg cccgccatca atcgctcccc gcccggctac gtttcctgac agaataccac
     9961 tgaccgtttc gcgctggccg caacatcccg ggtcgtcagt gcaacgcaga accgtccatg
    10021 gggaggatac gcacatgaag ccggtcaccc tgctcctcgt cctcgcggga agcctgctcc
    10081 ttcccggcgc gagcgccgcg cagccatacc cggcacgcac ggtccgcctg gtggtcggct
    10141 tcaccgccgg cggagcgacc gacatttccg cccgggccct cgcccagaag ctttcggggc
    10201 cgctcggcca gcaggtcgtg gtggagaacc ggaccggcgc ggcgagcacg ctcgcctccg
    10261 agttcgtcgc ccgcagcgcg cccgacggct acaccctgct gctcgcaaac gcgacggtcg
    10321 cgatgccttc cctcttcgcg aggctgggct tcgacgtgcg caaggacctc gcgccggtat
    10381 cgctggtggg atacggcccg ttggtgctgg tcgcccatcc ttccctgccc gtgaaatcgg
    10441 tgaaggagct gaccgcgctc gcccggcgca agcccggaat gctcaactac ggcaccgcgg
    10501 gggtcggcag catgacgcat ctcgcgatgg cgctgttcgt ctcgatgacg agcgtggaca
    10561 tggtgcacgt gccgtacaag ggcagctcgc aggcctcgat cggcgccatc accggcgaag
    10621 cgcagttgat gttcagctcg cccgccgccg tcatggccca gctccagcag gggcgactgc
    10681 gcgcgatcgc cgtctccggc acccggcgga gcgcggtgct gcccgacgtg ccgaccatag
    10741 ccgaagccgg gctcgccggc tacgacgcga cgtcgtggta cggcatgctg gcgccggcgg
    10801 cgacgccacg gccggcgatc tcccggctag gcgacgagag cgtgaaggcg ctagccgcct
    10861 cggacttgag ggagcggctc ctcgccttgg ggatcgagcc ggcgaaaggc ggcgtggacg
    10921 agttcgctgg ttatctcgaa gccgaaatcg ccaaatgggc gaaggtgatc aaggcggcgg
    10981 gcattccgcc gcaatgacga aggagcagaa aagccgaacc gccaaggacg cccaatatcg
    11041 gtgatgcgtg atgagtgatg ggtgacgagt cttccgcgcg ccgcgcggag cccatccgcg
    11101 acccgcgatc cgcgggcctt ttaagcacct gtcatctttc ccggccggct ggcgccgggc
    11161 ggaatttggc gcgataattt tccccgtcaa tcggctccga gcgcgaggag gagggcccgc
    11221 catgaacgcg ccgttgaaga aagtgaaggc gtcgatgatg aacgccattc acgacctgcc
    11281 cctgctggtc gcacgcgacg aaggctactt ccgggatgag ggcctggacg tggagatcgt
    11341 ccggacgccc ggttcgggcc agcgcgactc ggatcaccgg gcgctgcgcg ccaacatctt
    11401 cgagcgcacc atggaagcgc tctacgacca gggcgcctgc gaccagttcc ggatgtgcga
    11461 gtggggcgtc atgaagcgcg cggtggaatc caccgtcgcc ggcgggcatc gccccgcgaa
    11521 gatcgtggcc ctgggctcgg cgatgtcgtc gttcgcgatc atgaccgatc cgaaacagcg
    11581 catctacgaa cccgagcagt tgaaggaccg gcccgtcgcc gtcagcccct tcaacggctc
    11641 ccatttcacg acgttgaagc tgctcgaggg gttcctgaag cgcgagcaga tcaagaccgc
    11701 cttcgccggc acgatgaagg agcgcatgga agcggtcgcc cggggcgagg tggcggccgc
    11761 gagcctcatg gagccgtgga tcagcatcgc ggaaaagcgc gggctgcgtg ttctcatgga
    11821 gtcgcattcg acgcgcagcg aggccgccgg cgacgagctg gacggcccga cgctcgcgaa
    11881 aatgttccgc gccgaggcgg cggccgcggt ggcgatcgag aagaatcccg gacgctacgc
    11941 gcattacttc gtcgaggagg ccggcgggct gctcgagccc ggagacctga agctctcgcg
    12001 catcctcaac gcgccgcccc agccctatac ccgcgaccgc ttcgagcaca cctatcagtg
    12061 gacgctggga tggggcctgg ttcctccggg cgccacctac gaaaacacgg tcgataaccg
    12121 cgcctggcag taactcagta aagcgcctcc ttcaggcgct cgtacagccc ctcgtcgtat
    12181 tcctttccgt cgaaccgcgc gtgggtgagg tcggagatga tcgtgccgag gctcggcagg
    12241 ctgccgcgct cgaggtgccg ggccgggtcc cataactttg cgcgaaagat cgcgcgggcg
    12301 cactggaaga aaaccgtatc gacgcggacc acgagcaccg agcgcggcag cttgccctcg
    12361 acgcggaagc gcgcgagcag cgccggatcg gtgcggatct ccgcgcgccc gttgacgcgc
    12421 agcgtttcgc cgatgcccgg gatcaggaac agcagcgcca cgcgcgggtc ggcgatgatg
    12481 ttgcgcaggc tgtccacgcg gttgttgccg cgccggtcag ggatcagcag cgttttctcg
    12541 tcctcgacca cgacgaaccc gggcgcatcg ccgcgcggcg acgcgtccat gccgcccggg
    12601 ccaccggtag cgaggatgaa aaacggcgag gccgcgatca gcctctggta atgcggatgc
    12661 aggtagctga tctccttctc gacggcggcg cccgaggcca cgccgtagag cttttccagg
    12721 gtatcaacgt cggtgatccg gtgcgcttcg cttacggtga tcattgcaat gttcccccct
    12781 gcctgtcccg gcgaggcgtt ggacgcccgc tcgaagaaag aggtcattgt agccgctatc
    12841 gaatgggcga tgggttatgg accgcccgat ggatgaggga tgaccttgac gcttcgctcc
    12901 aggatgaggg tggaaggatg aaggatgacc aagggcgttt cccgtatgcc actcttggcg
    12961 ttgtcttcgc gtccttggat cggttttcga aaattatttc ttggcgtcct tggcggtgaa
    13021 gattttcttt tcctttgcgg atcagcgttc ttggcggtga ggctgttcct ttccttcacg
    13081 tccctggcgg ccttcgcggt aaagcttcga gcacgcccgc tccgcggtaa actcatcacc
    13141 catcacccgg tttaaggtca tccttcctcc ttccgccttc atccttttct tgggagagcc
    13201 catgcccccc tcgaacgacc tgcccgacat cctcgagacc accgaagccg aacgcctcgc
    13261 gaccggcttc accttcaccg aaggcccgct gtggcacccg gacggctact ggtacttcgt
    13321 cgacatccgc cagaacaagc tctaccggat gacgcccggg cgggcaccgg aagtgttcaa
    13381 ggaaacctcc ggaggaaacg gcacgacttt cgatctccag ggacgcctga tcctgtgcga
    13441 aggcacggcc cggcgcgtcg ttcgcatcgg ccacgacggc aaggtcgagg cgctggtcga
    13501 ccgctataag ggcgggcgct tcaaccgccc caacgacgtg gtgtgccgct cggacggcag
    13561 catctacttc accgacccgg acaagcgcgt gccctacgcc gagcgggaaa taccgggccc
    13621 tgccggcatt gacaacctgt gggacggcgc gagcgtctat cgtgtggcga ccgacggcgc
    13681 cttgagcgtg ctcgcccttt gcgaatatcc caacggcctc gcgttctcgc cggacgagcg
    13741 cacgctctac gtcgcgaaca cccgctcgtc gaagtacatc cacgcgctcg agctcga
//
LOCUS       DBNGWZ010000525        23987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1201710, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000525 DBNGWZ010000000
VERSION     DBNGWZ010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 23987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23987
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1201710"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="AC16CX_16190"
     CDS             <1..392
                     /locus_tag="AC16CX_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3014084.1"
                     /translation="GITAMRGFVVEPMQYGRLFLAGDAAHIVPPTGAKGLNLAVADVR
                     VLARAFADFYASGRINLLDRYSETCLRRVWKVQRFSWWMTSMLHRFHDEGTLRQRLQL
                     AELDYLTRSRAAATALAENYVGLPFEE"
     gene            complement(495..1646)
                     /locus_tag="AC16CX_16195"
     CDS             complement(495..1646)
                     /locus_tag="AC16CX_16195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014085.1"
                     /translation="MAFYLILLVALLNHSSFKGGKVLMSLFAIEHGASPATIGVLYAM
                     YSVFPVILSVYAGKLSDRYGFRLPVMIGSSGLFVALLLPYLIPDLVTLFVSASVAGMC
                     YIFYIVAIQHLIGAVGEGPERTRNYSLFSICVGLTSLAGPIVTGFSIDGIGHRATWLL
                     LAAFPALPILMLLALPALLPRPEHHERPAGRRHVLDLFGSVPLRRVLMATAFLETGME
                     TFNFLMPIYGHSIGLSASEIGIVMGVFGLALMVVRSAMPALVRRSGEERVFSVSMFVA
                     GAACIGFSLVASFIPLLAMSFVLGLGLGCGAPLSMTLSYNRAPAGRWGEAIGLRQTVN
                     KAIEATIPVVFGFVSTAFGVMPVYWLGALMLAGGGWLMDSDARQKAVTR"
     gene            complement(1817..2977)
                     /locus_tag="AC16CX_16200"
     CDS             complement(1817..2977)
                     /locus_tag="AC16CX_16200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014086.1"
                     /translation="MIIYLILFLGLLNWMCFMSSRVLMTLYAIQLGASAAAIGLLIAL
                     YGLGPLLLSVQAGKVSDRVGSYWPIVLGSAGLALGLAVPYFFSGLPVLYVSALVIGIA
                     LVFLNIALQHLIASLGDAEERTRNVSLQSLAIALGALIGPLLVGVSIDHRGHMPTFLY
                     LALIVAASCIAWIACRRVIPHSQRAAQTGAGGGVMGLLAQPQLRRVIIASGLVVAGID
                     LYTFYMPIYGHAIGLSATMIGVVLGAHAAATFVVRAVLPLLVRRWGEDPVMTASMLLA
                     GATFLLFPFVGQVAVLLLLSFVLGLGLGVGQPLSVIMTYNRAPAGRTGEALGLRFLLV
                     NLTHMVIPLAFGTIGSALGVATVFLANSALMLGGGYAHHRGATKKSSATRDL"
     gene            3080..3973
                     /locus_tag="AC16CX_16205"
     CDS             3080..3973
                     /locus_tag="AC16CX_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014087.1"
                     /translation="MQIRNVGVVSPGDMGQAIAGRLKESGLAVHTALDGRSERTRTLA
                     RAAGLTDCGSMEKLVATCELVISVINPGEALNVVRQIAAAMKKTGSKIALADLNAISP
                     QTAHEADRMIRDAGGLFIDGGIIGPPPRGEKDKPRIYVSGPDAYLFEQINHPNILVRV
                     LSERIGDASGVKMCYAAMTKGTTALAVELLVAARKLGVEQALEKELRESRNDVFEWQM
                     KNIATMPPKAYRWVPEMQEIAKTFGELGLTRRIFEGATDVYELVAATPLGKESPEQAR
                     KLGRTGLDVTRELADDSGGKR"
     gene            complement(4039..4953)
                     /locus_tag="AC16CX_16210"
     CDS             complement(4039..4953)
                     /locus_tag="AC16CX_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3014088.1"
                     /translation="MARQTRTLAVMFADISESTSLYQKLGDAAARNIVNACLAVINGV
                     LPRYDGRLVKTIGDEAMCVFPSADLALLAASEMQSLVASTKPGNYPVAIHIGLHYGPV
                     LVEENDVFGDTVNAAAYLRAVATAEQILTTEATEQCLSAALKSCVRPVFHAVLKGSTE
                     ESTVYQVLWRTDRLDITDVNLQSNKTIPGDTGSLLVTLDDERVRVDGWRTGIVIGRSK
                     DCDLVVTDRFASRQHLSIKLVRTHFYLFDHSINGTFVSLESGEEVHVLRRELLLDGSG
                     QICVGRSRTGRPPEVITFARDRRSMYRI"
     gene            complement(5044..5550)
                     /locus_tag="AC16CX_16215"
     CDS             complement(5044..5550)
                     /locus_tag="AC16CX_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014089.1"
                     /translation="MRFSYYNRLSPRQQSVYRRSDAIRTIRIPGGAPLEPLVREIGAA
                     LAADDRDIVQGACQALTDELARRFAVMRIEVSVLAKRPLLRGGGDLMGLYEPRERGKP
                     VTITLWMRTARRRQVVAFRTFLRTLLHEFCHHLDYEHHGLPDSFHTEGFYKRESNLMQ
                     QLLPPPKA"
     gene            complement(5644..6330)
                     /locus_tag="AC16CX_16220"
     CDS             complement(5644..6330)
                     /locus_tag="AC16CX_16220"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3014090.1"
                     /translation="MGEDLFENLRREMLAEIVTEAALTVGHTGRAAFSERIMRVIGDM
                     PRHEFVPVELQPYAYLNRPLPIGYEKTVSQPFIVALMTDLLELDKGDVVLEIGAGAGY
                     QAAILAELVKHVYTVDIIEELAQGAERRLKRLGYENVEVRVANGYYGWPEHAPYDKIM
                     VTAAADLIPPPLLAQLKAGGRMVIPTGIPDKQALVLVEKSASGSLSTREILPVRFSEL
                     EDPGETAGLA"
     gene            6439..7119
                     /gene="nfi"
                     /locus_tag="AC16CX_16225"
     CDS             6439..7119
                     /gene="nfi"
                     /locus_tag="AC16CX_16225"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: protein motif:HMM:NF008629.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR3014091.1"
                     /translation="MRKIRHSWNLTPAAAIALQHRLRSKVIRVGRPGQVRHVAGTDVG
                     FERGGRITHAAVAVLSFPGLELVDYAIARLPTRFPYVPGLLSFREIPALLVALEQLRV
                     RPDLILCDGQGLAHPRRFGIASHLGVYCDIPSIGVAKSRLIGEHGQPGQRRGAWVPLR
                     DKGETIGAVLRTRVNTRPVYVSIGHRVSLPLAVRYVMACATRFRLPETTRWAHRLASG
                     GGPIRDER"
     gene            7149..7973
                     /locus_tag="AC16CX_16230"
     CDS             7149..7973
                     /locus_tag="AC16CX_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR3014092.1"
                     /translation="MAISSASETNNLALFCDFENIALGVREARYAEFDVQKVLERLLL
                     KGNIVVKKAYCDWDRYKDFKKAMHEAAFELIEIPHVRMSGKNSADIRMVVDALDLCYT
                     KSHVDTFVIISGDSDFSPLVSKLRENDKTVIGVGVKKSSSDLLISNCDEFIYYDDLVR
                     ERPKKQGRRKPAAAAAGSDVGKPPAANGDKKQEALDLVMETIEALFSERGSEEKIWGS
                     MVKQALKRRHPGFNEAYYGFRSFGKLLDDAEARKLLTLEHDEKSGGVIIKSFNPDY"
     gene            8080..8349
                     /locus_tag="AC16CX_16235"
     CDS             8080..8349
                     /locus_tag="AC16CX_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014093.1"
                     /translation="MPGVHVNLRERSLLERIVYGMLLLAVLVLGFFFLVAALVAGVVL
                     AAAILLRIWWVKRKLKRTLEEEVITTEYTVVDRDQPSTPRLPPRQ"
     gene            8346..8921
                     /locus_tag="AC16CX_16240"
     CDS             8346..8921
                     /locus_tag="AC16CX_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014094.1"
                     /translation="MSHTLHRRVPFAWLCALLIAACQYPSAAAMASATLQDRNPPGWR
                     EFESNPGRFRVLLPGVPATSRQTIRTDIGNVASTRVTTTDAANVTYDILFNDYPRAGV
                     SKVSPQKLLDGARDGLLYQTKGRLIGEKPLTLANFPGRDQEIMGGDGTHYRIRLVWAG
                     NRLYQIMAVTPGQPRPDAQVFFDSFQIVGNR"
     gene            9119..9385
                     /locus_tag="AC16CX_16245"
     CDS             9119..9385
                     /locus_tag="AC16CX_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014095.1"
                     /translation="MTTFAQWRSPSSRLELARLGRKPKKYDIRVVDQFYVYCQAVART
                     DRHRDEAHVRSLHATKRTLWTILVTGSFLFYYVMDRIQVALSLL"
     gene            complement(9434..11791)
                     /locus_tag="AC16CX_16250"
     CDS             complement(9434..11791)
                     /locus_tag="AC16CX_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3014096.1"
                     /translation="MQITFDTIRKILERRLHQADIELLLHGLSDADKTQLFVRITELM
                     RRTSALVDIANRVSDTLSLDVLFPRLMEVVTETLNAERSSLFLYDPDTKELFSRVMQG
                     NAMGEVRFPADVGVAGSVFTSGEGEIIPDAYADVRFNREVDTRTGYRTRNILCVPIRN
                     KKHEVIGVTQALNKREGHFDVEDQQLLESLSSQAAAALENARLFEKVERAQREEAMFL
                     DVVSLIASEIYLDPLLEKILMAATQLLGADRGALFLYDPSRNELYSRVAGGVASRQIR
                     FPANAGIAGECFSRGVPVSIDDAYHDPRFNPEVDKGTGYRTRNILCMPISTKGGNKVG
                     VMEILNKKEGAFTFNDQRRLGAFCAQAAVSIENAQLFEDVSNSRNYNENILRSMSNGV
                     VTLDANDVVSKVNDSALRILRRSEAEIIGKAVRDIFGERNGWVTQSLEKVRNTGKIDI
                     AVDTDLLVEGREAVSVNLAAVPLHNRRDERIGSMLIMEDITREKRLRNTMSRYMSKAV
                     MDQLLESGDAVLGGTSREVSVLFSDIRGFTSLSERLGARETVVMLNEYFTDMVDVVFS
                     HNGILDKYIGDMIMAVFGSVVSREEDADNAVTVGSSMMVALRELNLRRTAAGHEPIRI
                     GVGISTGQVIAGNIGSPKRLDYTVIGDRVNLAERLENANKYYGTSVLICNTTVARLKR
                     SVKLRELDLIRLRGMRTPVSIYEVLDHHTSESFPRLDVVMAAFADGMVHYRKQSWSRA
                     AVSFSEALSACPGDRPSRIYLERCEIYRAEPPPPGWDGVWTIHTN"
     gene            complement(11807..13582)
                     /locus_tag="AC16CX_16255"
     CDS             complement(11807..13582)
                     /locus_tag="AC16CX_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3014097.1"
                     /translation="MNSPDPASASGQEAAPGVGLRALVERLAEEFSADPKTLARVASV
                     FGEVRERAPDLFVPAAGALAPAFAHIESSGGSNAELVYAALGEWALDAESEPIERMRR
                     DCMRFQSAMTRGALRLYAGEPERCAESLLALQRFIFLQLALLASLSARTQGEGGVVRT
                     LPTPEYAAFMEIFRTTIESHRQDDKQLALLLLQVARVEQVDRLLGLQKGEAFMLRVTR
                     RMREGVLRKQDQLGRVSRDQLACLLPRIAGEGVAILAANKILAALEAPIPIGDRSFAP
                     DAVIGIAMYPDHGADQQTLVRNGKLAVRVARGTAERVAVYEPAQGESEELKMRYEARL
                     RHALEEGTLDLAFEPQLDLHSGRIGGLECLLRWTDKELGKVSEARAMETAETSGIVRE
                     VTWWVFNTALRLSAEFARVGLQVPLGLKIAASGLLQPDFPEFMGRALRTWDIAPARVV
                     IEIHESALAEGINQVKETLGRLKAHGVRLGIDGFGTGSSSLINLAQLPLDEMKLSASF
                     VGDMQRTAPHAKIVRSLVHFARDLGLCVIAEGVADAETALALSTLGCERIQGEYVGPP
                     LTAQEIIAFEKSGAGLGGLSLRPQG"
     gene            complement(13590..14819)
                     /locus_tag="AC16CX_16260"
     CDS             complement(13590..14819)
                     /locus_tag="AC16CX_16260"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR3014098.1"
                     /translation="MKKQVSVEELEFGVYISELDRPWTETPFMFQGFELKSKDQLDVL
                     KQYCKKVVIDLEKGLDLPDRSRLKAGPAGPDGKLKSVLASIKRKAAYEEKTSVDVELP
                     RAREARSKTEVVLKDIVQMVKAGKALDAPRVKEAVTNMTDSVVRNPDAMLLLSKLKEK
                     GEHTLDRALGVSIYMITFGRFLQLPRDQLDLLGMLGLLQDIGKVRLPVDLLVKPEPLS
                     DAEREICKGHVAHSIAILKETPGLPTELPALAALHHERFDGSGYPKGLKGGEIGLFGV
                     IAGLIDCFDALTHPRPYGEALAPSNALSMLYGWRDAQFDGPLVEQFIQCIGIFPVGAI
                     VELNSGETGIVIAQNLARRLQPRVMVVLDAKGDPLRPQKVLDLAKNPKASPETPYRIK
                     RTLEKGSVPIDTSEFFL"
     gene            complement(14958..16139)
                     /locus_tag="AC16CX_16265"
     CDS             complement(14958..16139)
                     /locus_tag="AC16CX_16265"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HIR3014099.1"
                     /translation="MKKQVSVEELEFGVYISELDRPWTETPFVFQGFVLKDHKQLETL
                     KKYCKKVIIDLEKGMDLPDKSRLKAGPAGVRVLATIKEKVVYEEKASIKAEAPVARAA
                     HAKTAGLLKDMLDAVQAGKALDAPRVKEAVTSMTDSVVRNPDAMLLLTKMKEKSEHTL
                     DRALGVSIYMITFGRFLQLPRAQLDLLGMLGLLQDVGKVRIPEPLLEKKEPLSETERK
                     ICASHVEHSVAILKETVGLPPEIPELAALHHERYDGSGYPKGLRGGEIGLFGGIAGIV
                     DCFDALIHLRPYGEALSPSNALNTLYGWRDTQFDGPLVEQFIHCVGIFPVGAFVELYS
                     GEIGIVIAQNLVMRLMPRVMVVLDIRGSPIKPPRIVDRASVKRTLEKGSVSIDTSEFF
                     L"
     gene            complement(16239..17006)
                     /locus_tag="AC16CX_16270"
     CDS             complement(16239..17006)
                     /locus_tag="AC16CX_16270"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HIR3014100.1"
                     /translation="MHLRVLGCSGSIGGQHNRTTSFLLDQDVLIDAGTGVGDLSLAEL
                     TLIDHIFITHSHLDHVNSIAFFLDSVGALRPKAVTVYATGPTIETLKKHLFNWDIWPD
                     FTVIPTPEEPWLRYQEIEVGKVIVLGGRKITVLPAVHTVPAVGYLLDSGKGSLVFTGD
                     TGPNDALWSVVNRIENLKYLIIETAFSDKDRRLAELALHLCPSLLAAELAKLKRPAEI
                     YITHLKPSEVELTMQEIEELPGDRRPRPLLNNHVFEF"
     gene            complement(17185..17592)
                     /locus_tag="AC16CX_16275"
     CDS             complement(17185..17592)
                     /locus_tag="AC16CX_16275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR3014101.1"
                     /translation="MELSDKVKPGLTGTAEIVVGTRDTAPHVGSGKIGVLATPIMVNL
                     MEAAALQAVEKFMPPGHQTVGTHLDVKHFAATPVGLRVRAHAELVKIEGRTLTFRIHA
                     EDEREKIGEGVHERLIINVERFDQRMQKKLEKT"
     gene            complement(17710..18099)
                     /locus_tag="AC16CX_16280"
     CDS             complement(17710..18099)
                     /locus_tag="AC16CX_16280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014102.1"
                     /translation="MSDETRRAATNKYVVDLHRMQKKPAIGKVSTGEGASFNGERIYC
                     GLVTKKKGTGSKPHYHPDETFNYVLKGALKVNMDGQEFIVPTGCLLHIPANMVHTAVA
                     TDDGNATYLVWRDRMGEKQGKPTTVEV"
     gene            18492..19691
                     /locus_tag="AC16CX_16285"
     CDS             18492..19691
                     /locus_tag="AC16CX_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014103.1"
                     /translation="MKYSRTIHAILTMACTALICAAPATAQTIKIGFLSTYSGPGAAQ
                     GDQLDKGAKLYVKLNSDKLPPGVKIDLVVRDDTGPNPDNAKRVAQELIVRDKVQFLTG
                     FVWTPNAAAIAPLTAEAKVPYVSMNAAGVTIPTLSPYIARVSFTLWQSCYPLGQWAAK
                     RFKRVFVAVSDFAPGHEAEEAFMRGFKEGGGEIIGNVRIPLANPDFVPYMQRVKDAKP
                     DALFAFNPAGRQSTAMMKAYGELGLDKAGIKYIGPGDITTDEELQGMGDVALGVITAH
                     HYSAAADRPANKAFVAAYKKEYGEKLNPGFMAVGAWDAMDAIFYAIREQKGKVDPDRT
                     MELLKRYRNPNSPRGPISIDPDTRDIIQNEYLREVRKVGGQLANVEIETIATALKDPW
                     KEFNKKK"
     gene            19792..20658
                     /locus_tag="AC16CX_16290"
     CDS             19792..20658
                     /locus_tag="AC16CX_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3014104.1"
                     /translation="MFSSLISVSFHGVAYGMILYVISVGLSVTMGLMGFVNLAHGVFA
                     MAGGYVLTSAISGFGLPFPVALTLSFALVAAVSVVVERFLYSRLYGSDELEQVLFTIG
                     LIFMAVAVAKLIFGNLQQPVLLPDYLKGQFSLLGRDFPAYRVFLIAFSAGMIAALWFG
                     VERTLWGAMVRAAVDNVAMAQSVGINTRRLFTSTFALGSGLAGLGGALGAEIIAIQPT
                     YPFEHLAYFLIVVSIGGLGSLRGPFVAALLIGVTDTACKYWLPQFGAFFIYVATIGLL
                     LWRPAGLFGRRA"
     gene            20655..21626
                     /locus_tag="AC16CX_16295"
     CDS             20655..21626
                     /locus_tag="AC16CX_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3014105.1"
                     /translation="MSPVGYGANPRIRWTEWLPWLAAGACFFLLPDYLSLGARILTFI
                     LFALSLDLILGYAGIITLGHSAFFGLGAYVAGILSAKLGISDPALLLVAAAAAAGALG
                     IMTGAVIMRTRGLTQLMLTLAIAALCLEVAHKATPVTGGADGLSGIVIAPIFGLFKFD
                     MFGKTAYLWCLAILFAGWWLTRRIVYSPFGAALSGIRENVDRMHAVGTPVYRRLLAAY
                     TLSATIAGVAGALLTQTNQFVGLNVLGFEPSGELMVMLILGGVGRIYGAFVGPLVYLI
                     AQDLLSKQFPEYWYFGIGLILVLVVLFARGGLLGIADALLARLRRQP"
     gene            21623..22375
                     /locus_tag="AC16CX_16300"
     CDS             21623..22375
                     /locus_tag="AC16CX_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014106.1"
                     /translation="MTPVLETRGLCKNFGALTVAASVDFRLGAGARHALIGPNGAGKT
                     TFVNMLTGRLAPSSGKVLLAGEDVTRLAQAARVKLGLGRTFQINTLFRSMSVLDNVAL
                     GIAERHGVGARMWRPACAHGEVREEALELLTMLGIEDEAPRRILDLPYGKQRLVEIAI
                     ALGLRPKVLLLDEPAAGVPSLESGLILRALDRLPADIAILFIEHDMDLVFRFAQRITV
                     LVRGEVLCEGAPAEIANDPRVHQVYLGERRRG"
     gene            22368..23069
                     /locus_tag="AC16CX_16305"
     CDS             22368..23069
                     /locus_tag="AC16CX_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014107.1"
                     /translation="MAEALTLSAVRAGYGATVVLEDIGFTLPERSSLAVLGRNGVGKT
                     TLLATVMGHTTFHAGRIEYRGRSIARLPVYERWRLGIGYVPQTRDIFPSLSVEENLRV
                     AACSGRWTLERVYDLFPGVAGRRNHLGNQISGGEQQMLAIGRALMGNPSLLLMDEPLE
                     GLAPIIVEALLRALQRLIQEESLAVVLVEQHAELALEMTHDALVLNRGRIIHHGPSAE
                     LLADPARLTSLVVAA"
     gene            complement(23242..23541)
                     /locus_tag="AC16CX_16310"
     CDS             complement(23242..23541)
                     /locus_tag="AC16CX_16310"
                     /inference="COORDINATES: protein motif:HMM:NF045581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG0541 family transporter-associated protein"
                     /protein_id="HIR3014108.1"
                     /translation="MKLAFIVHNEYYTPKVMQLLKSAGIDYFTSWEKAKGKGHGTEPH
                     LGAGSFGSTNSVLMIAFQEEAPLDMLIREIAAANQEIKRPDDHIRLFQVPLERIV"
     gene            23734..>23987
                     /locus_tag="AC16CX_16315"
     CDS             23734..>23987
                     /locus_tag="AC16CX_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014109.1"
                     /translation="MRPKTALLLALAVPVAAFTPIRTMAVDSDLPPQPVSPEHAAGRK
                     AIQEKDWNTAIQSLTAAARRDTRNADVQNLLGYAYRNAGQL"
BASE COUNT         4379 a         7786 c         7398 g         4424 t
ORIGIN      
        1 agggaatcac cgcgatgcgc ggcttcgtcg tcgagccgat gcagtacggc aggctgttcc
       61 tcgccggcga cgccgcgcac atcgtacccc cgaccggcgc aaagggactc aacctggccg
      121 tcgccgacgt ccgcgtcctc gcgcgtgcat ttgcggactt ttacgccagc gggcggatca
      181 acctgctcga ccgctattcg gaaacgtgcc tgcgccgcgt gtggaaagtg cagcgcttct
      241 cctggtggat gacgtccatg ctgcaccgct ttcacgacga aggaacgctg cgccagcgtc
      301 tgcagctcgc cgagctcgat tacctgaccc gctcgcgggc tgccgcaaca gcgcttgccg
      361 aaaactacgt gggcctgcct ttcgaagagt gactgagtga ctgagtaact aggtgattac
      421 gtaagcggca accagtcatg tagtggcaaa gcaacgtcgc attcaacaaa ctgcgtgatc
      481 acctaattac ccaatcacct agtcaccgcc ttctggcggg cgtcgctgtc catcagccat
      541 ccgccgccgg ccagcatcag ggcgccgagc cagtacaccg gcatgacgcc gaaagcggtg
      601 ctgacgaaac cgaatacgac ggggatcgtc gcctcgatcg ccttgttcac ggtctgccgc
      661 aggccgatcg cctctcccca gcgtcccgcc ggcgcccggt tataggaaag cgtcatcgag
      721 agcggcgcgc cgcaacccag tcccaatccc aggacaaacg acatcgcgag cagcggtatg
      781 aagctcgcta cgagcgagaa gccgatgcat gcggcgccgg cgacgaacat ggaaacgctg
      841 aacacccgct cctcgcccga gcgtctgacg agcgccggca tcgccgagcg caccaccatc
      901 agcgcaaggc cgaagacgcc catcacgatt ccgatctccg aggcggacag tccgatcgaa
      961 tgcccgtaaa tcggcatcag gaaattgaaa gtctccatgc ccgtttcgag aaaagcggtg
     1021 gccatcagca cgcggcggag cggaacgctg ccgaacagat ccaagacgtg gcgccggccg
     1081 gccggccgct cgtgatgttc cggccgcggc agcagcgcgg gcagcgccag cagcatgagg
     1141 atgggcagag ccgggaacgc cgcgagcagg agccaggtcg cgcggtggcc gatgccgtct
     1201 atgctgaagc ccgtgacgat cgggccggcc agcgacgtca ggccgacgca aatgctgaac
     1261 aggctgtagt tgcgcgtgcg ctccggccct tcgccaacgg cgccgatcag gtgctggatc
     1321 gcgacgatgt aaaagatgta gcacatgccc gcgacggatg cggagacgaa cagcgtcacg
     1381 agatcgggaa tcaggtacgg cagaagcagc gcaacaaaca ggccggatga gccgatcatc
     1441 accggcagcc ggaaaccata gcgatcggag agtttcccgg cgtagaccga caggatcacc
     1501 gggaacacgg aatacatcgc atacagcacc ccgatcgtcg ccggactggc gccgtgctcg
     1561 atcgcgaaca gcgacatcag caccttgccg cccttgaaac tgctgtgatt gagcagcgcg
     1621 accagcagga tcaggtaaaa cgccacggct atccccgaag ccgccgccca attgaaacgt
     1681 caacgccttt gctcatcccc cgtgcttggt gacgggtgac tcgtgactgg tgactggaga
     1741 aagagcaatc attgttaagg ttcgtttggc aagccatgga tcgtctttca ttgttttgct
     1801 ttctgactga atcgagtcac aagtcacgag tcgcgctgct tttctttgtg gcgccgcgat
     1861 ggtgggcgta gccgccgccc agcatcagtg ccgagttggc gagaaacacg gtggccactc
     1921 ccagcgccga gccgatcgtg ccgaacgcga gcgggatgac catgtgagtc aggtttacca
     1981 gcaagaagcg cagcccgagc gcctctcctg tgcggccagc cggcgcgcgg ttgtaggtca
     2041 tgatgaccga gagcggctgt ccgacgccga gcccgagccc gaggacgaag gagagcaaca
     2101 gcaggacggc gacctgcccg acgaagggga acaggaggaa agtcgccccc gcgagcaaca
     2161 tcgaggccgt catcaccgga tcttcgcccc agcgacgcac cagcaacggc agcacggcgc
     2221 gcaccacgaa ggtcgcggcc gcatgggcgc cgagcacgac cccgatcatg gtggcggaca
     2281 ggccgatcgc gtgtccatag atcggcatgt aaaacgtgta gaggtcgatg cccgccacta
     2341 ccagaccgct cgcgatgatc acgcggcgca gttgaggttg tgccagcagg cccattacac
     2401 cgccgcccgc ccctgtctgg gccgcccttt gcgagtgcgg aatcacgcgg cggcaggcga
     2461 tccacgcgat gcacgaggcg gctacgatca gcgccaggta gagaaacgtc ggcatgtgac
     2521 cccggtggtc tatcgaaacg cccaccagca gtggcccgat gagcgcgccg agcgcgatcg
     2581 ccaggctctg caggctcacg ttgcgagtgc gctcctcggc gtcgccgaga gaggcgatga
     2641 ggtgctgcag cgcgatgttc aggaacacca gcgcaatgcc gatcaccagc gccgagacgt
     2701 acagcaccgg gagcccggag aaaaagtacg gaaccgctag tcccagcgca aggccggccg
     2761 accccagcac gatgggccag tagctgccaa cgcggtcgga aaccttgcca gcctgcaccg
     2821 aaaggagaag cggacccagc ccgtagagcg cgatgagcag gccgatcgcg gcggcggatg
     2881 cgccgagctg gatggcgtag agcgtcatca gcacgcggct gctcatgaag cacatccaat
     2941 tgagcagccc cagaaaaagg ataaggtaga taatcattaa aacggcgacg cggatcgtcg
     3001 ccgggcggga aaaatgttat tttagcgcaa gcttcatcgc gctctcacac gaaaccctcc
     3061 tgtcacgaaa ggaacacgca tgcagattcg caacgttggc gtcgtcagtc cgggcgacat
     3121 gggccaggcg atcgccgggc ggctcaagga aagcggcctc gccgttcata ccgcgctaga
     3181 cggccgcagc gagcgcaccc ggacgctcgc gcgcgcggcc ggcctgaccg actgcggctc
     3241 gatggaaaag ctcgtcgcaa cctgcgagct ggtcatttcg gtcatcaatc ccggcgaagc
     3301 gctgaacgtc gttcgccaga ttgcagccgc catgaagaag acgggaagca agatcgcgct
     3361 cgccgacctc aacgcgatct cgccgcagac ggcgcacgag gccgaccgca tgatccgcga
     3421 cgcggggggc ctgttcatag acggcggaat catcggtccg ccgccgcgcg gcgagaagga
     3481 caagccgcgc atctacgtct cggggccgga cgcctatcta ttcgagcaga tcaatcaccc
     3541 caacatcctg gtgcgcgtgc tgtccgagcg catcggcgac gcctccggcg tcaagatgtg
     3601 ctacgccgcg atgaccaagg gcacgaccgc gcttgcggtg gagctgctgg tggccgcgcg
     3661 caagctcggc gtcgagcagg cgctggagaa ggaactgcgt gaaagccgca acgacgtctt
     3721 cgagtggcag atgaaaaaca tcgccaccat gccgcccaag gcctaccgct gggttcccga
     3781 gatgcaggag atcgccaaga ctttcgggga gctggggctg acgcggcgaa tattcgaggg
     3841 cgcgaccgac gtctacgagc tggtcgccgc gacgccgctg ggcaaggaga gtccggaaca
     3901 ggcgcgcaag ctcgggcgca ccggcctcga tgtcacccgc gagcttgccg atgacagtgg
     3961 cggtaagcgg taagcggtaa gcggtaagtt tgatgcgact aggctgtcag cggttcaccg
     4021 ctcactgctc accgctcact agattctgta catcgaccgc cggtcgcgcg cgaaggtgat
     4081 gacctcgggc ggccgcccgg tgcggctgcg tccgacgcag atctggccgg agccgtccag
     4141 cagcagctcc cgccgcagca cgtgcacctc ttccccgctc tccagcgaga cgaaggtgcc
     4201 gttgatgctg tggtcgaaca ggtagaagtg ggtccgcacc agcttgatcg agagatgctg
     4261 gcgcgacgca aaccggtcgg tgaccaccag gtcgcagtcc ttcgagcggc cgatgacgat
     4321 accggtgcgc cagccgtcaa ctcgcacgcg ctcatcgtcc agcgtcacca gcaggctgcc
     4381 ggtatcgccg ggaatggtct tgttcgactg caggttcacg tcggtgatgt cgagacggtc
     4441 ggtgcgccac agcacctggt agaccgtgga ttcctccgtg ctgcccttca acaccgcgtg
     4501 aaagaccggg cgcacgcagg acttgagcgc cgccgaaagg cactgctcgg tcgcctcggt
     4561 ggtgaggatc tgctcggccg tcgccaccgc cctcagatag gcggcggcgt tcaccgtatc
     4621 gccgaacacg tcgttctctt cgaccaacac cgggccgtaa tgcaggccga tgtggatcgc
     4681 gaccggataa ttgccgggct tggtggacgc gacgagcgat tgcatttcgc ttgccgcgag
     4741 caacgcgagg tcggcgctgg ggaagacgca catcgcttca tcgccgatgg tcttgaccag
     4801 ccgcccgtca tagcgcggca gcacgccgtt gatcacggcc aggcaggcgt tgacgatgtt
     4861 gcgggcggcg gcgtcgccca gtttctggta gaggctggtg ctctcgctga tgtcagcgaa
     4921 catcaccgcg agggttcggg tctgtctcgc catgccgcgc gattataaac cccgtgaatc
     4981 gcgtttcgtg aatcgtcaac ggtttcctgt acgccatttg aatcacgaat caccaatcgc
     5041 caatcacgcc tttgggggtg gcagcagctg ctgcatgagg ttcgactcgc gcttgtagaa
     5101 gccctcggta tgaaacgaat ccggcagccc gtggtgctcg taatcaaggt gatggcagaa
     5161 ttcgtgcaac agcgtgcgca ggaacgtgcg gaacgccacc acctgccttc ggcgcgccgt
     5221 gcgcatccac agcgtgatcg tgacgggctt gccgcgctct cgcggctcgt agagacccat
     5281 caggtctccg ccgccgcgca gcaggggacg ctttgcaagc accgaaacct cgatgcgcat
     5341 caccgcgaag cgccgcgcga gctcatcggt cagcgcctgg cacgcgccct gcacgatgtc
     5401 gcggtcgtcc gccgccagcg ccgcgccgat ctccctcacc aacggctcga gcggcgcccc
     5461 gccgggaatg cgaatcgtcc ggatcgcgtc gctcctgcga tagaccgact gctgccgcgg
     5521 cgagagccgg ttgtagtagg aaaaccgcat tgaagcgcgg gccgtgaagc gtgattcgtg
     5581 agccttcgtg agatgctgga actacattcg aatcacgaat caccattcac gaatcacgct
     5641 gtgctaggcc aggccggcgg tttcgccggg atcttccagt tcggaaaagc ggacgggcag
     5701 aatctcgcgc gtggaaaggc tcccggatgc ggatttctcc accaggacga gagcctgctt
     5761 gtcggggatg ccggtcggga tcaccatgcg tccgcccgcc ttgagctggg cgagcagagg
     5821 cggcgggatg agatccgcgg cggcggtcac catgatcttg tcgtagggcg cgtgctccgg
     5881 ccagccgtaa tagccgttcg cgacccggac ttcgacgttt tcgtagccga ggcgcttcaa
     5941 ccgccgctcc gcgccctgcg ctagctcctc gatgatgtcc accgtataga cgtgcttcac
     6001 cagctcggcc agtattgccg cctgatagcc ggcgccggcg ccgatttcca gcacgacgtc
     6061 gcctttatcc agctccagca gatcggtcat cagcgccacg atgaagggct gcgacacggt
     6121 tttttcgtag ccgatgggta gcggccggtt gagataggcg tagggttgca gctcgaccgg
     6181 cacgaattca tgacgcggca tgtcgccgat gacgcgcatg atgcgttcgc tgaaagcggc
     6241 cctgccggtg tggccgacgg tgagcgcggc ttcggtcacg atctccgcca gcatctcgcg
     6301 gcgcaggttt tcgaacaggt cttcgcccat aatgcatagt actacactga tgagtgacaa
     6361 gtgataagtg tcatgacaag gcttggactc tccttccctt gtcaatgttc acgtatttga
     6421 cgtccaccgc gctggattat gagaaaaata cgccattcgt ggaaccttac gccggcagcg
     6481 gcgattgcgc tccagcaccg tctgcgatcg aaggtgatac gggtgggccg gccagggcag
     6541 gtgcgccacg tcgcgggcac cgacgtcggc ttcgagcgcg gcggcaggat cacgcacgcc
     6601 gcggtagcgg tgctgtcctt cccgggactg gagctggtgg attacgcgat tgcccgcttg
     6661 ccgacgcgtt ttccgtacgt gccaggcctg ctttcgttcc gcgagattcc ggcgttgctg
     6721 gtggcgctgg aacagctgcg ggtcaggccg gacctgatcc tgtgcgacgg acaggggctc
     6781 gcgcatccgc gccgcttcgg catcgccagc catctcgggg tgtactgcga cattccgagc
     6841 atcggcgtcg ccaagtcacg attgatcggc gagcacggcc agccggggca gcggcgcggc
     6901 gcctgggtgc cgctccggga taagggtgaa accatcggcg cagtcctgcg aaccagggtg
     6961 aatacgcggc cggtctacgt ttccatcggc caccgcgtga gcctgccgct ggcggtgcgc
     7021 tacgtcatgg cgtgcgccac ccgattccgg ctcccggaga cgacgcgctg ggcgcaccgg
     7081 cttgcctcgg gcggcgggcc gattagggat gagagataat gagcggctgg ataccacagg
     7141 agccctagat ggccatatca agtgccagcg aaaccaacaa tctcgcgctg ttttgcgatt
     7201 tcgagaacat cgcgctcggc gtgcgcgagg cccgctacgc ggaattcgac gtgcagaagg
     7261 tgctggaacg cctgctgctg aagggcaaca tcgtggtcaa gaaagcctac tgcgactggg
     7321 accgctacaa ggacttcaag aaggcgatgc acgaggcggc gttcgagctg atcgagatcc
     7381 cgcatgtgcg catgtccggc aagaactcgg ccgacattcg catggtggtg gacgcgctcg
     7441 acctctgcta taccaagtcg cacgtggata ccttcgtgat catcagcggc gactccgact
     7501 tctcgcccct ggtgagcaag ttgcgcgaga acgacaagac cgtcatcggc gtcggcgtga
     7561 agaagtcgtc ctccgatctg ctcatctcga attgcgatga attcatctac tacgacgact
     7621 tggtccgcga gcggccgaag aagcaaggcc ggcggaagcc cgccgccgca gctgccggct
     7681 ctgacgtcgg caagccccct gcggcgaacg gtgacaagaa acaggaggcg ctcgacctgg
     7741 tgatggagac gatcgaggcg ctgttctccg aacgcggctc ggaagagaag atctggggct
     7801 cgatggtcaa gcaggcgctc aagcgccggc accccggctt caacgaagcc tattacggtt
     7861 tccgctcgtt cgggaagctc ctcgacgatg ccgaagcacg caagctgctt acgctcgagc
     7921 acgacgagaa atcgggtgga gtcatcatca agagcttcaa tcccgactat tgaaaagcgt
     7981 gggtcgtgaa tcgtgattcg taaaagtgat tagtagcggc tcacggctcg tttcttttgg
     8041 tttaatcgat tcacgaatca ccaatcacgc accaataaag tgcccggcgt tcacgtcaac
     8101 ctgcgcgagc gcagcctgct ggagcgaatc gtttacggga tgctcctgtt ggcggtgctg
     8161 gtgctcggtt ttttcttcct cgtcgccgcc ctggtcgcag gagtggtgct ggccgcagcc
     8221 atcctgttgc gtatctggtg ggtgaagcgc aagctcaagc gaacgctgga agaggaagtc
     8281 atcaccaccg aatacacggt ggtcgaccgc gaccaaccgt cgacgccacg tctgccgccg
     8341 cggcaatgag ccacaccctc caccgccgcg ttccattcgc gtggctgtgt gccctgctga
     8401 tcgccgcatg tcagtaccca tccgcggcgg cgatggcgag cgccacgtta caggacagga
     8461 acccgcccgg atggcgggaa ttcgaatcaa accccgggcg cttcagggtg ttgctgccgg
     8521 gcgtgccggc cacgtccaga caaaccatcc ggaccgacat cggcaacgtt gcctcaaccc
     8581 gggttaccac gaccgacgct gccaacgtga cgtacgacat cctgttcaac gactatccca
     8641 gggccggggt gtccaaggta agcccccaaa aactcctgga tggcgcgcgc gacggattgc
     8701 tgtaccagac caagggccgg ctgataggcg agaaacccct aacactcgcc aatttccccg
     8761 gccgtgatca ggaaatcatg ggtggagacg gcacgcatta caggatcagg ctggtgtggg
     8821 ccgggaaccg tctctaccag atcatggcag tgactcccgg ccagccgcgg cccgatgcgc
     8881 aggtgttttt cgactcgttc cagatcgtcg gcaaccgcta gctgacgcgc tgatcctgag
     8941 tgacttgcct gggtgaatac catcagtgtg actttttccc tgtggaacgg aatttgcccg
     9001 atatcccatc aggaagcgga ctcgaaccgg gccggttaaa caaaagatta ttcgagtcaa
     9061 gtagttggat catagccggc gcgaagtacc cgcgcccaac cgtcgacaag agccacctat
     9121 gacgacattt gcacagtggc gttcgccctc cagccgcctg gagctggccc ggctcggccg
     9181 caaaccgaag aaatacgaca tccgggtagt ggatcagttc tacgtctatt gccaggccgt
     9241 cgcccggacg gataggcacc gtgatgaggc ccatgtccgg tcgctgcacg ccaccaagcg
     9301 aaccctctgg acgatactgg taacgggcag cttcctgttc tactacgtga tggacaggat
     9361 acaggtcgcg ctgtcgttgc tgtagcctcc cgcttcccgc atgcaagaag gaccgggagc
     9421 aatgctctcg gccctagttc gtgtggatgg tccagactcc atcccagccc ggagggggcg
     9481 gttcagcacg gtaaatctcg cagcgttcta agtaaatgcg cgatggtcga tctccggggc
     9541 aggcggagag cgcctcgctg aacgacaccg ctgcacggga ccaggactgc ttgcggtaat
     9601 ggaccatccc gtccgcaaag gcggccataa ccacatcgag acgggggaag ctttcgctgg
     9661 tgtgatggtc aagcacctcg taaatggaaa ccggagtccg catgcccctg agccggatca
     9721 ggtcgagttc tctcagcttc actgaacgct tgagccgcgc taccgtcgtg ttacagatca
     9781 gcacgctggt tccgtaatac ttgttcgcgt tctccagccg ctccgcgaga ttgacccggt
     9841 caccgatcac ggtgtaatca agccgcttgg gcgagccgat gttgcccgct atgacctggc
     9901 cggtgctgat tcccacgccg atgcgaatcg gctcatggcc cgccgccgtc cttcgaaggt
     9961 tcaactcgcg cagcgcaacc atcatactgc tgccgacggt cactgcgttg tctgcatcct
    10021 cctcgcgtga gaccacggac ccgaataccg ccatgatcat gtcgccgatg tatttgtcga
    10081 ggataccgtt gtgcgagaac acgacatcga ccatgtcggt gaagtattcg ttgagcatca
    10141 ccacggtttc ccgcgctcca agccgctcgg acagggaagt gaagccccgg atgtccgaga
    10201 acagcacgct gacttcccgg ctggtgccgc ccaggaccgc gtcgccgctt tccagcagtt
    10261 ggtccatcac tgctttgctc atatagcggg acatcgtatt gcggagccgc ttttcccggg
    10321 tgatatcctc catgatcagc atggaaccga tccgctcgtc ccgcctgttg tggagcggaa
    10381 ccgcagccag attgaccgaa accgcctccc gcccctccac caggagatcg gtatcaaccg
    10441 cgatgtcgat cttcccggtg ttgcgcactt tttccaggct ttgcgtcacc cagccgtttc
    10501 gctcgccaaa gatgtcacgt accgccttgc cgataatttc ggcttccgag cgccggagaa
    10561 ttcgcagtgc cgaatcgttg accttgctga ccacgtcgtt ggcatcgagc gtcaccacgc
    10621 cattactcat gctgcgcaat atgttttcgt tgtagttgcg ggaattgctc acgtcctcga
    10681 acaactgcgc gttctcgatc gataccgccg cctgagcgca gaatgcgccc agacgccgct
    10741 ggtcgttgaa cgtgaaagcc ccttctttct tgttgagtat ctccatcacg ccgaccttat
    10801 tgcccccctt ggtggagatc ggcatgcaca ggatgttgcg tgtgcgatag cccgtgccct
    10861 tgtccacctc ggggttgaaa cgcggatcgt ggtaggcatc gtcaatgctg acgggtaccc
    10921 cgcggctgaa gcactctccg gcaatgccgg cgttcgccgg gaagcggatc tgacgggacg
    10981 ccactccgcc cgcgacccgt gagtagagct cgttgcgcga ggggtcgtag agaaacaagg
    11041 cgccgcggtc ggcccccagc agctgagtgg ccgccatcag aatcttctcg agaagcgggt
    11101 cgagataaat ctccgaagca atcaggctca ccacgtcaag gaacatcgcc tcttcgcgtt
    11161 gcgcgcgctc gaccttctcg aatagccggg cgttctcgag cgcggcggcc gcctgggagg
    11221 acagcgattc gagcagctgc tggtcttcga catcaaagtg gccctctcgc ttgttgagcg
    11281 cctgggttac cccgatcacc tcgtgtttct tgtttcgaat gggaacgcaa aggatgtttc
    11341 gcgtccggta cccggtccgg gtgtcgacct cccggttgaa gcgcacgtca gcataggcat
    11401 cggggatgat ctccccttcg ccgctggtga agacggagcc cgctaccccg acatcggcgg
    11461 gaaaccgcac ttctcccatc gcattgccct gcatgacccg ggagaagagt tctttcgtgt
    11521 ccgggtcata aaggaacagc gagctgcgct ccgcgttgag ggtctcggtc acgacttcca
    11581 tcaggcgggg gaacagcaca tccagcgaca gcgtgtccga cacccgattg gcgatgtcga
    11641 ccagcgcgga ggtgcggcgc atgagctccg tgatccggac aaagagttgc gtcttatcgg
    11701 catcggacag gccgtgaagc agtagttcga tatccgcctg gtgcagccgg cgctccagga
    11761 ttttccggat ggtatcgaaa gtgatttgca tatccgaaaa gcggcttcaa ccctgcggtc
    11821 taagcgacag tccgcctaac cctgccccgc ttttttcgaa ggcgatgatt tcctgcgcgg
    11881 tcaggggggg gcccacgtat tcgccctgga tgcgctcgca accgagcgtt gacagcgcca
    11941 acgcggtctc agcgtccgcc acgccttcag ctattacgca cagcccgagg tcacgcgcga
    12001 aatggaccag cgaacgcacg atcttcgcgt ggggcgccgt tcgctgcatg tccccgacaa
    12061 acgatgcact gagcttcatc tcgtcgagcg gcagttgcgc caggttgatc agggacgacg
    12121 aaccggttcc gaagccatcg atacccagac ggaccccatg cgccttcaag cgcccgagcg
    12181 tttccttcac ctgattgatc ccctcggcaa gcgctgattc gtggatttcg atcactaccc
    12241 gagccggggc aatgtcccag gttcggagcg cgcggcccat gaactcggga aaatcgggtt
    12301 gcagcaaacc gctcgctgcg atcttcaggc cgagcggaac ctgaagcccg acacgggcga
    12361 actcggcgct tagtcttaac gcagtattga acacccacca cgtcacctcg cggactatcc
    12421 cggaggtttc ggctgtctcc atcgcccgcg cctcagacac cttgccgagt tccttgtccg
    12481 tccagcgcaa taggcattcg agtccgccga tccggcccga atgcaggtcg agctgcggct
    12541 cgaacgcgag atcaagcgtg ccctcttcca gcgcgtgacg cagccgcgct tcgtatcgca
    12601 tcttgagctc ttcgctctca ccctgcgccg gctcgtagac agcgacccgc tccgcggtgc
    12661 cgcgcgcgac gcgcacggcg agcttgccgt tgcgaaccag cgtctgctgg tccgcgccgt
    12721 ggtccgggta catcgcgatg ccgatcaccg catccggggc gaacgagcgg tcgccgatcg
    12781 gtatcggcgc ttccagcgcg gcgaggatct tgttggcggc cagaatcgcc acgccctcgc
    12841 cggcgatgcg cggcaggagg caggcgagct ggtcacggct cacccggccg agctggtcct
    12901 gcttgcgcag caccccttcg cgcatgcgcc gggtaacgcg cagcatgaag gcttcaccct
    12961 tttgcaaccc gagcaggcga tccacctgct cgactctcgc gacctggagc aggagcaacg
    13021 caagctgctt gtcgtcctgc cggtggctct cgatcgttgt gcggaagatc tccatgaagg
    13081 ccgcgtattc tggcgtcggc agcgtccgca cgacgccgcc ttctccctgg gtgcgcgcag
    13141 aaagcgaagc gaggagcgca agctgaagga agatgaagcg ctgcaacgca agcaggctct
    13201 ccgcgcatcg ctccggctct ccggcgtaca accgcagcgc gccgcgcgtc atcgcgctct
    13261 ggaagcgcat gcagtcgcgc cgcatgcgct cgatcggctc gctctcggcg tccagcgccc
    13321 attcgcccag cgccgcatag accagctcgg cgttgctgcc gcccgaagac tcgatatgcg
    13381 cgaacgcggg cgcgagcgcc ccggcggccg gaacgaacag gtccggcgcg cgctcgcgca
    13441 cttccccgaa taccgacgcg acgcgggcaa gggtcttcgg atcggcgctg aactcctccg
    13501 ccagacgctc gaccagggcc ctgaggccga cgcccggcgc cgcctcctgc ccggaagcag
    13561 aggccggatc cggcgagttc attccgccct cagaggaaga attcggacgt gtcaatcggg
    13621 acactccctt tctccagcgt gcgcttgatg cgatacggcg tctccggaga cgccttcgga
    13681 ttcttcgcca gatcgaggac cttctgcggc ctgagcggat cccccttcgc gtcgagcacc
    13741 accatgacac gcggctgcag ccgccgcgcg agattctgcg cgatcacgat cccggtctcg
    13801 cccgagttca actcgacgat ggctccaacc ggaaagatcc cgatgcactg gatgaactgt
    13861 tcgaccagcg gaccgtcgaa ctgggcgtcg cgccagccgt acagcatgct gagcgcgttc
    13921 gagggcgcga gcgcctcgcc ataggggcgg ggatgcgtga gcgcgtcaaa gcagtcgatg
    13981 agtcccgcga tgacaccgaa cagcccgatc tccccgccct tcaggccctt cggatagccg
    14041 ctgccgtcga agcgctcgtg gtgcagggca gcgagcgcag gcaattcagt gggaagcccc
    14101 ggggtctcct tgagaatcgc gatcgagtgc gcgacgtgcc ccttgcagat ctcccgctcg
    14161 gcatcgctca ggggctccgg cttcacgagc agatcaaccg gcagacgcac ctttccgatg
    14221 tcctgcagca gcccgagcat tcccagcaga tcgagctgat cccgcggcaa ttgcagaaat
    14281 cgtccgaacg tgatcatgta gatggagacc cctaacgcgc ggtcaagggt gtgctcgccc
    14341 ttctccttca gtttgctgag caggagcatc gcatcaggat tgcgcacgac gctgtcggtc
    14401 atgttcgtta ccgcttcctt cacgcgaggc gcgtcgagcg ctttaccggc cttgaccatc
    14461 tgtactatgt ccttcagcac gacttcggtc ttgctccgcg cttcgcgcgc gcgcggcagc
    14521 tccacatcga cgctcgtctt ttcctcgtac gcggcctttc tcttgatgct cgcgagcacg
    14581 ctcttgagct tgccatcagg gccagccggg ccggccttca gccggctccg gtcaggcagg
    14641 tccaggccct tttccaggtc gatgacgacc ttcttgcagt attgcttcaa cacgtcgagc
    14701 tggtctttgc tcttgagttc gaatccctgg aacatgaacg gcgtttccgt ccagggccgg
    14761 tcgagctcgg agatgtacac cccgaattcc agttcttcga cggagacttg ttttttcatg
    14821 atgatcgctt caatcccgtg gcggcagctt ccacggcgca acgggtagga ttttccgtcc
    14881 tgccggggcc gcatgcaagc gcccacctcc ggttccagcc cggaagcaga tcccgggtcc
    14941 tgtccctcca cccgcgctta aaggaagaac tcggaggtgt ctatcgaaac actgcccttt
    15001 tccagcgtgc gcttgacgct cgcccgatcg acgatcctcg gcggcttgat cgggcttccc
    15061 cttatatcaa gcaccaccat gacgcgaggc atcagtcgca tcaccaggtt ctgcgcgatg
    15121 acgatcccga tctcgcccga gtacagctcg acgaaagccc ccaccggaaa gatcccgacg
    15181 caatggatga actgttcgac caggggcccg tcgaactggg tgtcgcgcca gccatagagc
    15241 gtgttgagcg cgttggaggg cgaaagcgct tcgccgtaag gccggaggtg aatcagcgcg
    15301 tcgaagcagt caacaatgcc cgcgatgccg ccaaacagcc cgatctcccc gcccctcagg
    15361 cccttcggat agccgctgcc gtcatagcgc tcgtggtgca gggccgcgag ctcgggtatt
    15421 tcggggggaa gcccgacagt ttccttcagg atcgccaccg aatgctccac gtgggaggcg
    15481 cagatcttgc gctcggtttc gctcaagggc tccttcttct cgagcaatgg ttccggtatg
    15541 cgcactttcc cgacgtcctg caacaggccg agcatgccga gcagatcgag ctgcgcgcgg
    15601 ggcaactgca gaaaacgccc gaacgtgatc atgtagatcg agacccccaa tgcgcggtcg
    15661 agggtatgct cgctcttctc cttcatcttg gtgagcagca acatcgcgtc gggattgcgc
    15721 acgacgctgt cggtcatgct ggtgacggcc tccttcacgc gaggcgcgtc gagcgcctta
    15781 ccggcctgga ccgcgtcgag catgtccttc agcaggcccg cggtctttgc atgagccgcg
    15841 cgcgccaccg gcgcttctgc tttgatgctc gccttttcct cgtagacgac cttttccttg
    15901 atggtcgcca gcacgcggac gccggccgga cctgctttca gccggctctt gtcgggcagg
    15961 tccatgccct tctccaagtc gatgatcact ttcttgcagt atttcttcag cgtctcaagc
    16021 tgtttatggt ccttgagcac gaagccctgg aacacgaacg gcgtttccgt ccagggccgg
    16081 tcgagctcgg agatgtacac cccgaattcc agttcttcga cggagacttg ttttttcatg
    16141 gcggcgccaa tcccgtggcg gcaggtccca cggcacaaac cggtaagatt ttccgtcctg
    16201 ccggggtcgc atgcaagcgg cccagcgaca aggcggcatc agaattcgaa tacgtgattg
    16261 ttcagaagcg ggcgcggccg ccgatcgccg ggaagctcct cgatctcctg catcgtcagt
    16321 tcgacctcgc tgggcttgag gtgcgtgatg tagatttccg ccggccgctt gagctttgcc
    16381 agctccgcgg cgagcaggga tgggcacagg tgcagggcca gctcggcaag ccggcggtcc
    16441 ttgtcggaaa acgcggtttc gatgatgagg tacttgagat tctcgatccg gttcaccacg
    16501 ctccaaagcg cgtcgttggg gccggtgtcg ccggtgaaga ccaggctccc ctttccgctg
    16561 tcgagcaggt agccgacagc aggaaccgtg tggaccgccg gcagcaccgt gatcttccgc
    16621 ccgccgagga cgatcacttt ccccacctcg atttcctggt agcgcaacca tggctcctcg
    16681 ggcgtgggga tcacggtgaa atccggccag atgtcccaat tgaacagatg ctttttcaga
    16741 gtctcgatcg tggggcccgt tgcgtacacc gtgaccgcct tcggccgcag cgcgccgacc
    16801 gaatccagga agaatgcgat cgagttgacg tggtcgagat gcgaatgggt gatgaagatg
    16861 tggtcgatga gcgtcagctc ggcgagcgaa agatcgccga cgccggtccc cgcgtcgatg
    16921 agaacgtcct ggtcgagcag gaacgaagtg gttcgattgt gctgcccgcc gatactgccc
    16981 gagcagccga gcacgcgcag gtgcattgcc gctcccgcgc ctatttgaga tcaaatgaaa
    17041 ggatcgtcag atccacgccc ccggccccct cgtcgtattt gttaccgtcc actggtgaat
    17101 tcacatctta ttataactgt cgatcaattg cttcaagttt cgaccaggtt acccgtacaa
    17161 gtccggttaa gccatccaaa atggtcacgt tttctcaagt ttcttctgca tgcgctggtc
    17221 aaaccgctcc acgttgatga tgagccgctc atgaacgccc tccccgatct tctcgcgctc
    17281 gtcttcagca tgaatcctga aagtcagagt gcgcccttcg atcttcacca gttccgcgtg
    17341 cgcccgcacg cgcaagccga cgggtgtcgc cgcgaaatgt ttcacgtcga gatgcgtgcc
    17401 caccgtctga tgccccggag gcatgaactt ctcgaccgcc tgcagcgccg cggcttccat
    17461 gagattcacc atgatcggcg tggcgagcac gccgatcttg ccgctgccca cgtgcggcgc
    17521 ggtgtcgcgc gtgccgacga cgatttcggc ggtcccggtc agcccaggct tcaccttgtc
    17581 ggaaagttcc atcatccctg gctttctagt gccgttccaa ctgttcgtgc caggccaacc
    17641 ctgtctccgg tagccggctg tccctggcag gtgtgaagcc aatacctgga gaaacgagtt
    17701 ggaacggcac tagacctcca ccgtcgtcgg cttgccctgc ttctcgccca tccggtcgcg
    17761 ccagacgaga taagtggcgt tcccatcgtc cgtggccacg gcggtatgca ccatattggc
    17821 cgggatgtgc agcaggcagc cggtcggcac aatgaactcc tgcccgtcca tgttgacctt
    17881 caacgcgcct ttcagcacgt aattgaaggt ttcgtcggga tggtagtgcg gtttggaacc
    17941 ggtgcccttc ttcttggtga cgaggccgca atagatccgc tcgccgttga atgacgcacc
    18001 ctcccccgtt gagacctttc caatagccgg tttcttctgc attcgatgaa ggtccaccac
    18061 gtatttgttc gtggctgctc tgcgggtttc gtcggacatg ccggctgctc ccttgcctga
    18121 accgctttcg ctatcctgcc aataaagtga gactgtcgcc agcgttattt ccgcctcgtc
    18181 catacttgtt ggaacggcac caggatcggc gctccccggc cccttcctta ccccgagaag
    18241 agcgggtggt atctcgtctg ccagttcgtg cgtcatgccg cagaccaggt ttagacaata
    18301 tgccgcccgg ccattcgcca agacgcgatc gcgtcccggg gtcttgtcat tgtgcacccg
    18361 tccgcgccac aatcagcctc ctgacgcagt aaaccgagtt gggcggcttg agcgaggccc
    18421 ggcattcgcg ccagaccggc aagctcgttc gtcaggaatc cggcaatcct attatcctcg
    18481 gaggaggggt tatgaaatac tcgagaacca tccacgccat cctcacaatg gcctgcacgg
    18541 ctttgatctg tgccgcccct gcgactgccc agaccatcaa gatcggcttc ctcagcacct
    18601 acagcgggcc gggcgccgcc cagggcgacc agctcgacaa gggcgccaag ctctacgtca
    18661 agctgaacag tgacaaactg ccgcccggcg tgaagatcga cctggtggtg cgcgacgata
    18721 caggaccgaa cccggacaac gccaagcgcg tcgcgcagga actgatcgtg cgcgacaagg
    18781 tgcaattcct gaccggcttc gtgtggacgc ccaatgcagc cgcgatcgcg cctcttacgg
    18841 ccgaggccaa ggtgccatat gtcagcatga acgccgcggg cgtgaccatt ccgacgctct
    18901 cgccgtatat cgcgcgcgtg tccttcaccc tgtggcaatc gtgctatccc ctcgggcaat
    18961 gggcggccaa gcggttcaag cgcgtcttcg tcgccgtcag cgatttcgcg cctggccacg
    19021 aggcggagga agctttcatg aggggcttca aggaaggcgg cggcgagatc atcggcaacg
    19081 tgcgcatccc gctcgccaat ccggacttcg tgccctacat gcagcgcgtc aaggacgcga
    19141 agccggacgc gctgttcgcg ttcaatcccg ccggcaggca atcgaccgcg atgatgaagg
    19201 cgtacggcga actcggcctg gacaaggccg gcatcaagta cattggcccc ggcgacatca
    19261 ccaccgacga ggagctgcag ggcatgggcg acgtcgcgct cggcgtcatc accgcgcacc
    19321 actactcggc cgcggccgat cggccggcca acaaggcctt cgtcgccgcc tacaagaagg
    19381 agtacggcga gaagctcaac cccgggttca tggcggtcgg cgcctgggac gcgatggacg
    19441 cgatcttcta cgcgatccgc gagcagaagg gcaaagtgga cccggaccgg accatggaac
    19501 tgctcaagcg ctaccgcaat ccgaacagcc cgcgcggccc gatctcgatc gaccccgata
    19561 cccgcgacat catccagaat gaatacctgc gggaagtgcg caaggtgggc ggccagctcg
    19621 ccaacgtcga gatcgagacc atcgccacgg cgctgaagga cccgtggaag gaattcaaca
    19681 agaagaaata gtcccgcgga cgacgcagga gccgatcgcc aacccccgcg gcacgcgcgt
    19741 cgtgcgcggg ggttttgttc gtccgctgtg cggatcgaag gcatggacgc cctgttctcg
    19801 tcgctgatca gcgtatcgtt tcacggcgtc gcctacggga tgatcctgta cgtgatatcg
    19861 gtcggcctct cggtaacgat gggcctgatg gggttcgtca acctcgcgca cggggtgttc
    19921 gcgatggcgg gcggctacgt tctcacctcc gccatcagcg gcttcggcct gccgttcccg
    19981 gtcgcgctca cgctgtcctt cgccctcgtg gccgcggtca gcgtggtagt cgagcgcttt
    20041 ctctattcgc gcctctacgg ctccgacgag ctcgagcagg tgctgttcac catcggcctc
    20101 atcttcatgg cggtggcggt ggcgaagctg atcttcggca acctgcagca gccggtgctc
    20161 ctcccggatt acctgaaagg ccagttctcc ctgctcggcc gtgacttccc ggcgtaccgg
    20221 gtgtttctga tcgcattcag cgcggggatg atcgcggcgc tgtggttcgg cgtcgagcgt
    20281 acgctatggg gcgcgatggt gcgcgccgcg gtggacaacg ttgcgatggc gcaatcggtc
    20341 ggcatcaata cccgacggct gttcacgagc accttcgcgc tgggcagcgg cctcgccggc
    20401 ctgggcggcg cgcttggcgc cgaaatcatc gcgatccagc ccacctatcc gttcgagcac
    20461 ctggcctatt ttctgatcgt ggtttcgatc ggcgggctgg gcagcctgcg cgggccgttt
    20521 gtcgcggcgc tgctgatcgg cgtcaccgac accgcctgca aatactggct gccgcagttc
    20581 ggcgcgttct tcatctacgt cgcgaccatc ggcctgctgc tgtggcggcc ggccgggctg
    20641 ttcgggaggc gggcgtgagt cccgtcggat acggcgccaa tccgcgcatc cgctggaccg
    20701 agtggctgcc ctggctcgcc gcgggcgcgt gtttcttcct gctgcccgac tatctgtcgc
    20761 tcggggcgcg catcctgacc ttcatcctgt tcgcgctctc gctcgacctc attctcggct
    20821 acgccggcat catcacgctc ggccacagcg cgttcttcgg actcggcgcc tacgttgccg
    20881 gcatactctc cgccaagctc gggatcagcg acccggcgct cctgctcgtc gccgccgcgg
    20941 ctgccgccgg cgcgctcggc atcatgaccg gggcggtgat catgcgcacc cgcgggctca
    21001 cgcagctcat gctcacgctc gcgatcgccg ccttatgcct ggaagtcgcg cacaaggcga
    21061 cgcccgtcac cggcggcgcc gacggccttt caggcattgt gatcgcgccg atcttcggct
    21121 tgttcaaatt cgacatgttc ggcaagaccg cttacctgtg gtgtctcgcc atcctgttcg
    21181 ccggctggtg gctcacgcgc aggatcgtct actcgccgtt cggcgcggcg ctcagcggaa
    21241 tccgcgagaa cgtcgaccgc atgcacgcgg tcggcacgcc cgtctaccgg cggctgctgg
    21301 cggcctatac gctgtcggcg accatcgcgg gcgtggccgg ggcgttgctc acgcaaacca
    21361 accagttcgt cggcctcaac gtgctcggct tcgagccctc gggcgagctg atggtgatgc
    21421 tgatactggg cggcgtcgga cgcatctacg gcgccttcgt cgggccgctc gtgtacctga
    21481 tcgcacagga tcttctctcc aagcagttcc ccgaatactg gtatttcggc atcggcctga
    21541 tactggtgct ggtggtactg ttcgcccgcg gcgggctgct cgggatcgcc gacgccctcc
    21601 tcgcgcggct gcggagacag ccgtgacccc ggtgctcgag acgcgcggct tgtgcaagaa
    21661 cttcggcgcg ctgaccgtgg ccgccagcgt cgatttccgt ctcggcgccg gcgcgcgcca
    21721 cgcgctgatc ggtcccaacg gcgcgggcaa gaccacgttc gtcaatatgc tcaccggccg
    21781 gctcgcgccc tcgtccggaa aggtgctgct cgccggcgag gacgtgaccc ggctcgcgca
    21841 ggcggcgcgc gtcaagctcg gcctgggacg gaccttccag atcaacaccc tgttccgcag
    21901 catgagcgtt ctcgacaacg tggcgctggg catcgccgag cgccacggcg tgggcgcgcg
    21961 gatgtggcgg ccggcttgcg cccacggcga ggtgcgcgag gaggcgctcg agctgctgac
    22021 catgctcggg atcgaagacg aggcgccgcg gcgcatcctc gacctgccct atggcaagca
    22081 acggctggtc gagatcgcga tcgccctcgg cctcaggccc aaggtccttt tgctcgacga
    22141 gccggccgcc ggcgtaccgt cgctcgaatc cgggctcatc ctgcgggcgc tcgaccgcct
    22201 gcctgcggac atcgccatcc tgttcatcga gcacgatatg gacctggtgt tccgcttcgc
    22261 ccagcgcatc accgtgcttg tgcggggcga ggtgctgtgc gagggcgcgc ccgcggaaat
    22321 cgcgaacgac ccgcgtgtgc atcaggtcta tctcggagag cggcggcgtg gctgaggcgc
    22381 tgacgctgag cgcggtgcgg gcgggctacg gcgcaaccgt ggtgctggag gacatcggct
    22441 tcacgctgcc cgagcgcagc tcgcttgcgg tgctcgggcg caacggcgtg ggcaagacca
    22501 cgctgctcgc caccgtcatg ggccacacca ccttccacgc cggccgcatc gagtatcggg
    22561 gccggtccat cgcccgcctg ccggtgtacg agcgctggcg gctggggatc ggctacgtgc
    22621 cgcagacgcg cgacatcttt ccatcgctct cggtcgaaga aaacctgcgg gtcgccgcct
    22681 gttccggccg ctggacgctc gagcgcgtct acgatctttt tccgggcgtc gccgggcgac
    22741 gcaaccactt gggcaatcag atctcgggcg gcgagcagca gatgctcgcg atcggccgag
    22801 cgttgatggg taatccctcg ctgctgctca tggacgagcc gctggagggc ctagccccga
    22861 tcatcgtcga agccctgctg cgggcgctgc agcgtctgat tcaggaagag tccctggcgg
    22921 tagtactggt ggaacagcac gcggagctcg cgctggaaat gactcacgac gccctggtgc
    22981 tcaaccgcgg ccgcatcatc catcacgggc cgagcgccga gctgctggcc gacccggcgc
    23041 gccttaccag tctggtcgtc gcggcctagg agttcgaccg attttgcggt gacaaactcc
    23101 tagcaaccgg tcgaagcgcc aagtccgttg cccatcattc attacccttc cgcctctaag
    23161 agtccctgac ataatgaatc acacgctgcg ctgtcacgat tttggctcgc accgcacgcg
    23221 cgtttcatta tgtcagggac tctaaacgat gcgctcgagc ggcacctgga acaggcgaat
    23281 gtgatcgtcg ggccgcttga tctcctgatt ggccgccgcg atctcacgaa tcagcatgtc
    23341 aaggggagcc tcttcctgga acgcgatcat caacaccgag ttggtgctgc cgaagctccc
    23401 cgcgccgagg tgcggttccg tgccgtggcc ttttcctttt gccttttccc agctcgtaaa
    23461 gtaatcgatg cccgcggact tgagcaattg catcaccttc ggagtgtaat attcgttgtg
    23521 tacgataaat gccagcttca tggtgccagt tctccgaaat cacccgacgc caaaagtcta
    23581 cggccctgga ggggcgaggt gttgatttgg gtcaaccccc tgcattgccg tttgtccacc
    23641 ccggcgggcg ggaatagagg acggccaacg cgtgtttacc ggtcgactcg ctcgtatcat
    23701 ataccacccg aattcgctta tcggagccga cacatgcgcc cgaaaaccgc actgctgctg
    23761 gcgttggccg tccctgttgc ggcgttcacg cctatccgga caatggccgt tgacagcgat
    23821 ctcccaccac aacccgtcag ccctgagcac gccgcgggaa ggaaagcgat ccaggagaaa
    23881 gactggaaca ccgcgatcca atcactcacg gccgcggcgc ggcgcgacac gcgcaacgcc
    23941 gacgtccaga acctgctggg ctacgcgtat cgcaacgcag gccagct
//
LOCUS       DBNGWZ010000526         1585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_198641, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000526 DBNGWZ010000000
VERSION     DBNGWZ010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_198641"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1319
                     /gene="glmU"
                     /locus_tag="AC16CX_16320"
     CDS             3..1319
                     /gene="glmU"
                     /locus_tag="AC16CX_16320"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3014110.1"
                     /translation="MPKVLHPLAGRPLIAHVVETARALKPARICVIYGHGGEQVPQAV
                     PADDLAFIKQQPQLGTGHALAQALPQLGTGGLTLVLYGDVPLIGEETLAAMIAGGGDR
                     LTILTTELDDPSGYGRIVRNRRGSITAIVEEKDASAAQRAIREVNTGIMALPTSYLAG
                     WLGRLTDRNAQREYYLTDVVALALANRVPVATVKARSTWEVLGVNSKQQLAQLERVYQ
                     WQRATRLMDAGVTLADPMRMDVRGNLACGADVRIDVNCVFEGEVKLADRVEIGANCIL
                     RDTEVGPGTRIEPFTYIDAARIGANCRIGPYARLRPGARLAQEVHIGNFVEVKEAEIG
                     ARSKANHLAYVGDATVGHDVNIGAGTITCNYDGANKHRTVIEDDVQIGSDVQLVAPVT
                     VGKGATIGAGTTVWKPAPPGELTVNEKTQVTKPGWKRPQKKVRSEE"
BASE COUNT          292 a          541 c          528 g          224 t
ORIGIN      
        1 gcctgcccaa ggtgctgcac cccttggcgg gacgcccgct catcgcgcac gtggtcgaga
       61 ccgcgcgcgc gttgaagccc gcgcgcatct gcgtgattta cgggcatggc ggcgagcagg
      121 tgccgcaggc ggtcccggcc gacgacctcg cgttcatcaa gcaacagcct cagctcggca
      181 ccgggcacgc cctcgcccag gcgctgccgc agctcgggac cggcggactc acgctggtgc
      241 tctatggtga cgtgccgctg atcggggaag agacgctggc ggcgatgatc gcgggtggcg
      301 gcgaccgcct gacgatattg accaccgagc ttgacgaccc ctccggctac ggccgcatcg
      361 tgcgcaaccg ccgcggcagt atcaccgcca tcgtcgagga aaaggatgcc agcgccgcgc
      421 agcgcgccat ccgggaagtc aacaccggca tcatggcgct gcccacttcg tacctcgcgg
      481 gctggctggg ccgcctgacc gaccgcaacg cgcagcgcga atactacctg acggacgtcg
      541 tggcgctggc gctggccaac cgggttccgg ttgcgaccgt caaggcgcgt tccacctggg
      601 aagtcctcgg ggtgaacagc aagcagcagc tcgcgcagct cgagcgcgtc taccagtggc
      661 agcgcgcgac gcggctcatg gacgccggcg tcacgctcgc ggacccgatg cgcatggacg
      721 tgcgcggcaa cctcgcctgc ggcgccgatg tccgcatcga cgtgaattgc gtgttcgagg
      781 gcgaggtgaa gctcgccgac cgcgtcgaaa tcggcgccaa ctgcatattg agggataccg
      841 aagtcggccc gggcacgcgt atcgagccgt tcacctacat agacgcggcc aggatcggag
      901 cgaactgccg catcggaccc tatgcgcgtc tgcgtccggg cgcccgtctc gcacaggagg
      961 tgcacatcgg aaacttcgtc gaggtgaagg aagccgaaat cggcgcccgc tccaaggcca
     1021 accacctggc atacgtcggc gacgcgaccg tcgggcacga cgtcaacatc ggtgccggca
     1081 ccatcacctg caactacgac ggcgccaaca agcaccgcac cgtgatcgag gacgacgtgc
     1141 agatcggctc cgacgtgcag ctcgtcgcgc cggtcacggt cggcaagggc gccacgatcg
     1201 gggcgggcac caccgtgtgg aagccggcgc cgccgggcga gctgaccgtg aatgaaaaaa
     1261 cccaggtcac caagccgggc tggaaacggc cccagaagaa agtgaggagt gaggagtgag
     1321 gcgcgaggcg tcaagacaaa cgaggtggcg agccatacgt ttcgcgacaa aggtctgtct
     1381 cctcacccct cacccctctc gcctcacggg gttctagcat gtgcggcatc gtcggcgcgg
     1441 cctcgacccg caatgtcatc gatcttctga tcgagggcat caagcggctc gaataccgcg
     1501 gctacgactc gaccggcatc gcctacgtca agggcagccg gcttgagcgg ctgcgctcca
     1561 ccggacgcgt tgctcatctg gacga
//
LOCUS       DBNGWZ010000527        16567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_446104, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000527 DBNGWZ010000000
VERSION     DBNGWZ010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 16567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16567
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_446104"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="AC16CX_16325"
     CDS             <1..1295
                     /locus_tag="AC16CX_16325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007740643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3014111.1"
                     /translation="LKVAALARERIGGMQGIEAEAPKPRVTMVGHRWDNACGELRQFL
                     ARNRITYDWMTPDAPELPTRWPGTRPADEDCPALKLADGTLLSRPAKRDLAQRLGLQT
                     SARLAEYDTIIVGGGPAGLAAAVYGASEGLRTLVVEREAPGGQAGTSSRIENYLGFPS
                     GVSGDELASRALKQAGRLGAEILVTRSVARIDPKTRQIYLDGDDVVRARTIILATGVT
                     WRRLAIEGFDRLIGKGIYYGAARSEASATHGLDVHLIGAGNSAGQAALYFANHARTVT
                     LVVRGDSLEKSMSRYLIEQLAGKSNVKVHLRSEVVCAHGDTHLTAIDVRDDASGEVRR
                     VDCGGLFVFIGADAETGWLPADIARDKRGYVLTGDDVVKAGRWPLSRDPYLLESSVPG
                     VFACGDVRLSPVKRVASAVGEGSMAIAFVHKYLQQDAR"
     gene            1472..2521
                     /locus_tag="AC16CX_16330"
     CDS             1472..2521
                     /locus_tag="AC16CX_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM domain-containing protein"
                     /protein_id="HIR3014112.1"
                     /translation="MSLYSIIHDGKEIREGFRARVLKPAIRFDSIKAAPLTTNYRIVG
                     TAFDYLLRFFLQRINPSAQMSMWVAEEGVELIGASEYASGFEQSESYQRADRERRKAD
                     TFLNEAKRQHQAYLKTGVVTDDLLFAVLRLAYLDVAYRAGPDRVDWVGLESPDANDTA
                     DLRALISLVNEPSFRATHVCLLNPTFGAASGLVGGADADLLLDDCLIDIKTTKDPRFD
                     VRDFFQLIGYYLLHGFGGIHCGNSKTTHEINSLAIYFSRYGYLWKVAVDEVLPPGSVP
                     DTARWFFESVCTSKAQRLKYTRGFRGPLAEHLITQEKSEKPKSKRRSPSKKGRKSLMS
                     NPRFHTDARKSGARR"
     gene            2559..3317
                     /locus_tag="AC16CX_16335"
     CDS             2559..3317
                     /locus_tag="AC16CX_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3014113.1"
                     /translation="MDPVIRAMAMAGLMFLSAPDFANAAEIKIFSTRAIATVLDKVGP
                     EFERTTGHRLNVTTDIAIRMVRRIQAGEPFDFLVAAPGQIDGLINEGKIIPETRTNLT
                     RSGIGVQVRAGAPKPDISTVEAFKHALLNAKSIAYLKEGQSGVYLAGLLERLGIAEAI
                     QSKVTRPDTDIVSELVAKGQIELGMVVITQILTTPGVELVGPLPPEIQSYITFTGGVS
                     TASRAPNAAKELIRFLTGPAALPVIRSQGMEPAL"
     gene            complement(3537..3779)
                     /locus_tag="AC16CX_16340"
     CDS             complement(3537..3779)
                     /locus_tag="AC16CX_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014114.1"
                     /translation="MSLYVVRAFVRMREVLATHKELAKKLEALEKKTEALALKHNALA
                     TTTRAQFKEVIEALRVLMTPPEPKRRPIGFITPDEK"
     gene            complement(3776..4033)
                     /locus_tag="AC16CX_16345"
     CDS             complement(3776..4033)
                     /locus_tag="AC16CX_16345"
                     /inference="COORDINATES: protein motif:HMM:NF022018.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HIR3014115.1"
                     /translation="MLDADLAALYRVETRVLGQAVKRNLERFPKDFMFQLNYQEVAAL
                     KSQAVISNPPGRGGRRSAPYASPSTGLLWLPPPSTRRAQSK"
     gene            complement(4178..5026)
                     /locus_tag="AC16CX_16350"
     CDS             complement(4178..5026)
                     /locus_tag="AC16CX_16350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3014116.1"
                     /translation="MAEGVELALGAMMFYGLADWVYKRATASGVQAHHFLPLQAFFFA
                     PGIFLYGLATGTLVLGVPFAWGMMAGVLVFVALYNFARSLASGAVSIVVPVFRLSFAI
                     TAALAVWLLDEPLTGWKLAGLAAALTAVWLLLAGGPAPAPRATPSSVLRVIVATVAMG
                     IVSLIYKIGTLAGGSPATVLTGQASVFLPLATLFALARDRGFHPPPGAWRHAATAAVL
                     LLFGLVMLLAGLARGEASVLVPVAQLSFVVTAGLGFAFLRETLTLRKGCGLAFAIAAL
                     ICLARS"
     gene            complement(5049..6059)
                     /locus_tag="AC16CX_16355"
     CDS             complement(5049..6059)
                     /locus_tag="AC16CX_16355"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR3014117.1"
                     /translation="MSGERQAASPILDACVTRARAARKRLVFPEGHDERIVAAARRLK
                     DQGIASPVLLGAGGEIAAAAARANVSLDGITLLDPVESDRLEAYAGLYAAGRPSASAK
                     VAERLVRKPLFHAGMMVKAGDADAMIAGATVATGRVIEAGLMTVGLTGGIRNPSSFFL
                     MIVPGFGGASERGFIYADCAVNVDPDPETLADIALASAESCRAVLREEPRVALLSFST
                     KGSAQHARVEKVTRALAIARERAPDLAIDGELQADTALVPAVAAKKLKGASPVAGRAN
                     VLVFPDLDAGNIGYKLTQCLAGARAIGPVLQGFARSVSDLSRGATAEDIVATAAIVLA
                     RA"
     gene            complement(6108..6809)
                     /locus_tag="AC16CX_16360"
     CDS             complement(6108..6809)
                     /locus_tag="AC16CX_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3014118.1"
                     /translation="MIDVYSWPTPNGHKVHIMLEETGLQYRVHGVNIREGDQFRPEFL
                     KISPNNRIPAIVDHEGPVGRPISLFESGAILLYLASKTGKLLPADMHARWTCVQWLMW
                     QMGGIGPMFGQANHFRRYSKEKIQYAIDRYTNEANRLTRVLDHRLGEARYTAGDDYSI
                     SDIAIFPWMRGAENRGVDLSEYPNVKRWFDEISTRPAVQRGVKVLAEVQAANQAPHSD
                     KSWEIMFGKVQFQRR"
     gene            6999..8234
                     /locus_tag="AC16CX_16365"
     CDS             6999..8234
                     /locus_tag="AC16CX_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014119.1"
                     /translation="MSRSASASWLLPPDTLPEARVLLAARALRALGDGYVSLLLPYYL
                     TLLGFSALEVGVIATATLLGSGLMTLGVGIIAHRFPGRDLLRLACLIMIATGVAVVLV
                     TDFWPLLLVAFLGTLNPSSGDVSVFLPLEHSQLARTVEARSRTALFARYSLIGALVGA
                     LGAQAAALPQLFSALLGVPEKSSIQAMFLLYGLFGAASFALYQRLPTRLEARQEERPV
                     PLGKSKGVVYTLAALFSLDAFAGGFAVQSLLALWLYERFGLSPAAAGTIFFWTGVLTA
                     FSYPVAAWLAARIGLVNTMVFTHLPANVFCILAALMPTLPLAVLFLCLRAALSSMDVP
                     ARTSYVMAVVSPAERPAAAGITAVPRSLAAAVSPAFSGYLLSLTPFGWPLVICGALKI
                     LYDLTLLGMFRKVKPPEER"
     gene            complement(8330..9319)
                     /locus_tag="AC16CX_16370"
     CDS             complement(8330..9319)
                     /locus_tag="AC16CX_16370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014120.1"
                     /translation="MAEPFPSKPVRIVIPYPISGPTDIRGTARMSRSYRLMAPNAPPA
                     ISDTLARAAAQAIRADTRQPVLLKRQPGGITTRGAMSVARASADGHTLLLASNATMVI
                     NPNYFYGVEYDPARDFVLVAPLATMPFVLLVRSDLTVETPQALINWLRPRPGEVNYGS
                     SGDGSTGHLAGELFRRMAHVNIIHVSFNGGVAALNGLAARQVSLMFAALPLALAYFPN
                     EYFRPLAITSPRRFERLPGLPTLAESGLPEFDLEGWYGLFAPAGSPPVATAWLRERIS
                     TAIADPATQMQLMALGLEPAAMPLEQFATRINTENEKWAPVLRASRLPLKDGA"
     gene            complement(9409..10839)
                     /locus_tag="AC16CX_16375"
                     /pseudo
     CDS             complement(9409..10839)
                     /locus_tag="AC16CX_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            11079..11957
                     /locus_tag="AC16CX_16380"
     CDS             11079..11957
                     /locus_tag="AC16CX_16380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014121.1"
                     /translation="MRIVIGFTPGGVPDITARTIAAKLTERWRQQVIVDNRTGAGGTI
                     AAQIVASANPDGYTLLSVSNAHSVAPAIYARLPYDTLKDFAGITMTANGPALLLVSPS
                     IGVKSAKDLIALARARPGQLNFSSAGVGSGTHFGGELFKSQAGIDVVHIPFKGIPEAI
                     TETMTGRVHFFLSPLASAIGLVKEGKVPAVGVTSLKRIPQLPDIPTVSESGLPGFRWD
                     YWYGMLAPAKIPRPLLNQLNQEITDILNQPDVRSRWTALGAEPAPTTSQAFDKFIAED
                     IATLTKIARAANIKAN"
     gene            12118..13281
                     /locus_tag="AC16CX_16385"
     CDS             12118..13281
                     /locus_tag="AC16CX_16385"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR3014122.1"
                     /translation="MSVTDLPGWTLKGRRLLPIVQGGMGIGVSAHRLAGSVARLGALG
                     TISSVELRHHHPDLLGLAKETGDTDALDRMNLTALDREIRQALEIAQGAGAVAVNVMK
                     AVALHAAYVRQSCESGAHAIVMGAGLPLDLPDMTKDFPDVALMPILSDARGTGIVVKK
                     WMRKNRLPDAIVIEHPRYAGGHLGATRSEEIRDLRFDFERVVEGIHGLLRDLGIERER
                     IPLVLAGGINTHDKVRALLALGASAVQIGTPFAVTVEGDAHPDFKRVLAEARPEDIVE
                     FMSVAGLPARAVLTPWLRHYLQREKALQSHAKADPRRCVPKTKCLTVCGLRDGIASIG
                     QFCIDMRLAYALKGDIKKGLFFRGSESLPFGQAIRPVTELIEYLLTGRMPEFA"
     gene            complement(13282..13647)
                     /locus_tag="AC16CX_16390"
     CDS             complement(13282..13647)
                     /locus_tag="AC16CX_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014123.1"
                     /translation="MASRQEEIKALKAGEPLPSHRIVQLRSMGMHSVRFEFVVRLLRS
                     SLKVDTLSIYWEHGTEFMLKREVEDVRRHLVLGRRKRVTGDFPDLWLLCYPDDEEIKQ
                     SVERELDHMVEQVREHDVP"
     gene            complement(13844..14302)
                     /locus_tag="AC16CX_16395"
     CDS             complement(13844..14302)
                     /locus_tag="AC16CX_16395"
                     /inference="COORDINATES: protein motif:HMM:NF018908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HIR3014124.1"
                     /translation="MPVERISITSSVRLAMAIAATHIAAAALLWIVPIPVVGKAVFTL
                     AVAVSLIFFMARDAALHAAHSIVALELRDTGGIAFQTRRGDWVDSEVLGSSYVSPRLT
                     IVNLRPQGRWKTRRVILMPDNVDPRDFRRLRMWLRWKQGEGREPLPEGER"
     gene            complement(14304..15026)
                     /locus_tag="AC16CX_16400"
     CDS             complement(14304..15026)
                     /locus_tag="AC16CX_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3014125.1"
                     /translation="MEEDYRAPDVLAEGDSWFSYWIPGNGNLIDRFNRDIWKDRYTIL
                     CLAYPGDEAIKMVDGRSRWVLQETLKAYSTIRMLLFSGGGNDIAADNLLKLLRRDCSG
                     AANHRECFRDGQPGRRLAEIEHAYRDLVTLRDMYRPGAVIVTHNYDYAVPGKKLLWMA
                     WLDPYMELARVPKQFREDIVAEFIDGFAKMLSGLKGPGFEFVRTAGTLKKSDWANELH
                     PNLVGFRKIARRFRRTLLKHLP"
     gene            complement(15055..15267)
                     /locus_tag="AC16CX_16405"
     CDS             complement(15055..15267)
                     /locus_tag="AC16CX_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014126.1"
                     /translation="MLLWLGLVTAPGPAVAANCVDDCKRRLQARIGNCNLLFEDKDSA
                     YYRNTRWHGECVSRARADFDSCRSSC"
     gene            complement(15273..15533)
                     /locus_tag="AC16CX_16410"
     CDS             complement(15273..15533)
                     /locus_tag="AC16CX_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014127.1"
                     /translation="MQYLMVLLVIIGVLMLGVAALLAISYGLGALMYSRDGIRADPSD
                     ADPCAQCHADRDWFEGLPIWKQNVVTAWWWANRYTCAAKGCR"
     gene            complement(15783..16466)
                     /locus_tag="AC16CX_16415"
     CDS             complement(15783..16466)
                     /locus_tag="AC16CX_16415"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIR3014128.1"
                     /translation="MRRAATAALLAPIRAIHARMGARNFAWFLLCALGTGLAGCLAPG
                     NLPPREYYVLDDLAKTAASKRATAIDRVLLVNPTSVSPFYDTQSLAFSRAPGQRAHYQ
                     FAAWTERPGRRFSELLMRRLDARGGFKSVTSTTSGVTGDLVLNTRLEEFYQDAGTDPG
                     SARIELSAELIDHARRAILARRAFSQSAPAGGEGASSAVAAFNRALTALLDEVTVWLE
                     REAARPAPR"
BASE COUNT         2934 a         5455 c         5242 g         2936 t
ORIGIN      
        1 ccctgaaggt cgccgcgctg gcgcgcgagc gcatcggcgg gatgcagggc atcgaggccg
       61 aggcgcccaa gccgcgcgtc accatggtcg gtcaccgctg ggacaacgcc tgcggcgagt
      121 tgcgccagtt cctcgcgcgc aaccggatta cctacgattg gatgacaccc gatgcgcctg
      181 aactgccaac gcgttggccc ggaacccgtc cggcggatga agattgccct gcgctgaagc
      241 tggctgacgg gacgctcctc agccgacctg cgaagcgtga ccttgcccag cgccttggcc
      301 ttcaaacgag cgcacgcctt gctgaatacg acaccatcat cgtcggtggc gggccggcag
      361 gtctcgcggc ggcggtatac ggcgcatccg agggactgcg caccctcgtg gtagagcgcg
      421 aggcacccgg cggccaggca gggacgtcct cgcgcatcga gaactatctc ggtttcccca
      481 gcggcgtctc gggcgacgaa ctcgcgagtc gggcactcaa gcaagcaggg cggctcgggg
      541 cggagattct ggtcacgcgt tctgtcgctc gcatcgatcc gaaaacgcgt cagatttacc
      601 tcgatggcga cgatgtcgtc cgggcgcgga ccatcattct tgcaaccggc gtcacgtggc
      661 gccgcctcgc gatcgaaggc ttcgatcggc tcatcggcaa gggcatctac tacggagcgg
      721 cacgcagcga agcgagtgcg acccatggac tcgacgtcca cctcattggt gccggaaact
      781 cagccggtca ggccgctctg tacttcgcca atcacgcgcg cacggtgacg ctggtcgtcc
      841 ggggcgattc tctggagaag agcatgtccc gctacctgat cgagcagctc gccggtaagt
      901 cgaacgtgaa ggtccatctg cgttcggaag tggtctgcgc gcacggcgac acccacctga
      961 cggcgatcga cgtgcgcgac gacgcgagcg gcgaggtgcg ccgggtcgac tgcggcggcc
     1021 tgttcgtgtt catcggcgcc gacgcggaaa cgggctggtt gccggcggac atcgcgcgcg
     1081 acaaacgagg atacgtgctg accggcgacg acgtggtgaa ggccgggcgc tggccgctca
     1141 gccgggatcc ctacctgctc gaatcgagtg tccccggcgt tttcgcttgt ggggatgtaa
     1201 ggctgagccc ggtcaagcgc gttgcctccg cggtcggtga gggcagcatg gcgatcgctt
     1261 tcgtccacaa atatctgcag caagacgcgc ggtagcactc agcaagcacg gtgaagcgta
     1321 tgcgtgtttc ccccgggccg aaggtgctgc ggtcagcgtc acgcccaacc atgcgatgca
     1381 gcggacctct tcacaccggg cctacgcccg ctgtttcgag gccactgatc gcagacgtta
     1441 gacatcaaca agacgaccgg cagcacaacc catgagcctc tattccatta ttcatgacgg
     1501 gaaggagatt cgcgagggat ttcgcgcacg cgtgttaaag cctgcgatac gcttcgattc
     1561 cataaaggcg gcaccactca cgacgaacta tcggattgtc ggtaccgcct ttgactacct
     1621 tctccgattt tttcttcagc gtattaatcc tagtgcgcaa atgtccatgt gggttgccga
     1681 agagggcgta gaacttatcg gtgcttccga gtatgcatcc ggcttcgagc agagtgagtc
     1741 atatcaaaga gcggatcgtg agcgcagaaa agctgacact ttcctcaatg aggctaaacg
     1801 tcagcatcaa gcgtatttga agactggcgt tgtcacggac gacctcctgt ttgccgttct
     1861 acgtcttgcc taccttgatg ttgcctatcg agctggcccc gatcgcgttg actgggtagg
     1921 tcttgaatca ccggatgcaa acgacaccgc agatttgcgt gcgttgatct ctctcgttaa
     1981 tgagccatct tttagggcga cgcacgtgtg tctgctaaac ccaacgttcg gtgcagcatc
     2041 tgggctcgtc ggcggagcgg atgcagacct ccttctcgat gactgcctca tcgatattaa
     2101 gactacgaaa gaccctcggt ttgatgttcg ggattttttc cagttgatcg gttattacct
     2161 tcttcacggg ttcggcggta ttcactgcgg gaattcgaag acgacacacg aaatcaactc
     2221 gctagctatc tatttctccc gctacggtta cctttggaag gtcgccgtcg acgaagtatt
     2281 gccacccggc tctgtgccgg acaccgcaag gtggtttttt gaatcggtat gcacctcaaa
     2341 agcacaacgg ctcaaatata cgcgcggctt tcgcggtcca cttgccgagc atcttattac
     2401 tcaagaaaaa agtgaaaagc caaaatcaaa gcggcgctca cctagcaaga agggaagaaa
     2461 atcattgatg tctaaccccc gcttccatac ggacgcgcgc aaaagcggcg cgcgccggtg
     2521 aaacggaacg ttagagcgca acggaaagga gagaagaaat ggacccagtg attcgcgcca
     2581 tggcgatggc ggggctaatg ttcctttccg ctccggactt cgcaaatgcc gccgagatca
     2641 agatcttcag caccagggcg atcgccactg tcctggataa ggttggtcca gagttcgagc
     2701 gcacaacggg gcaccggctc aatgttacga cggatatcgc gattcgcatg gtgcggcgga
     2761 tacaggccgg cgagccgttt gattttctcg tcgccgcgcc cggacagatt gatggactta
     2821 taaacgaggg caagatcatc cccgagacac gtaccaatct cacccgttcc ggcatcggcg
     2881 ttcaggtccg cgcgggtgct cccaagcctg atatcagtac cgtcgaggcg ttcaagcatg
     2941 ctttgctcaa tgcaaaatct atcgcttatc tcaaagaagg ccagagcggt gtgtatttgg
     3001 ccggcctgct ggagcgcctc gggatcgccg aagccatcca gtctaaggtc acccgcccgg
     3061 acaccgacat cgtttcggag ctcgtcgcca aaggacaaat cgagcttggg atggtggtga
     3121 tcactcagat cctgacgacg cccggcgttg agctcgttgg cccactgccc cccgaaatcc
     3181 agtcctacat cacattcacc gggggcgtaa gtacagcctc aagagcgccg aacgcggcca
     3241 aagaattgat ccggttcctc actggcccgg cggcccttcc agtgattaga tcgcaaggca
     3301 tggaacccgc gctctaacaa ccgcctgcag ctgacgccct acagcctccg cttcgctacg
     3361 gctttcgggc gcagctgagg cgtggcgtta aatgtccgct atattgggtc gaaagcagac
     3421 gttcgatctg aaccgctacc gccacgcttt cccgataaac aacgttacgt ggtccggccc
     3481 tgaggtttcc gcataaatca tggctggcat cgcgcaggcg ttgtgatctt gggcggctac
     3541 ttctcatctg gcgtgatgaa gccgatgggc cgcctcttcg gctccggtgg cgtcatgagc
     3601 acgcgcagcg cctcgatcac ttccttgaac tgcgcgcgcg tggtggtggc gagcgcgttg
     3661 tgcttgagcg caagcgcttc ggtcttcttc tccaacgctt ccaatttctt ggcgagttct
     3721 ttgtgcgtgg cgagcacctc gcgcatgcgc acaaaggcgc gcacaacgta cagactcatt
     3781 tcgactgcgc gcggcgagtt gagggcggtg gcagccataa gagccccgtg ctcggtgaag
     3841 cgtagggcgc gctgcgtcgg ccgcctcggc cgggcgggtt tgaaatcaca gcctgtgatt
     3901 tcaaagctgc aacctcctga taattcaact gaaacatgaa gtccttgggg aagcgttcga
     3961 ggttgcgttt caccgcctgc ccgagcaccc gggtctcgac gcgatagagc gcagccaagt
     4021 cggcatcgag catgaccttg tgaccgcgca cgatgagaat cgcgcgctcg atgcgttcga
     4081 ctgggaccgg gactgagctc atggcgcttc aaagtgaaca acatcattaa cacgccagcc
     4141 tcaaaaaagt gaacccggcc ccatttacag gcagcggtca gctgcgcgcg aggcagatga
     4201 gtgctgcgat ggcgaaagcc agcccgcaac ccttgcggag cgtcagcgtc tcgcgcagga
     4261 aggcgaaacc gagtccggcc gtgaccacga agctcagctg cgcaaccggc acgagcacac
     4321 tcgcctcgcc gcgcgccagg cccgcgagca gcatgacgag cccgaacaag agcagcaccg
     4381 cggccgtggc cgcgtggcgc caggcgccag gtggtggatg gaatccgcgg tcgcgcgcga
     4441 gcgcgaacag cgtcgcgagt ggcaagaaca cgctggcttg gccagtgagg accgtggcag
     4501 ggctgccacc ggcaagcgtg ccgatcttgt agatgaggct cacgattccc attgccaccg
     4561 tcgcgacgat gacgcgcaac acggacgatg gggttgcacg cggtgccggc gccggcccgc
     4621 ccgcaagcag cagccacacc gctgtgagcg cggctgcgag gccagcgagc ttccagcccg
     4681 taaggggctc gtcgaggagc cacaccgcga gcgctgccgt gatcgcgaag ctcagccgga
     4741 agaccggcac aacgatgctg accgcgccgc tcgcgaggct gcgcgcgaag ttgtagagtg
     4801 cgacgaagac cagcacgcca gccatcatgc cccatgcgaa gggcacaccc aggacgagcg
     4861 tgccggtcgc gagcccgtag agaaaaatgc ctggcgcgaa gaagaaggcc tgcagcggca
     4921 gaaaatgatg agcctgcacg ccgcttgcag tggcgcgctt gtagacccag tccgcgagtc
     4981 catagaacat catggcgccc agcgccagtt ccacgccttc agccatgccg gctccaccct
     5041 aatagctgct acgcccgcgc gaggacgata gcggcagtcg ccacgatatc ttccgcggtt
     5101 gcaccgcgcg aaaggtcgct caccgaccgt gcgaatccct gcagcaccgg gccgatcgcg
     5161 cgcgcgcctg ccaggcactg ggtgagcttg tagccgatat tgcccgcatc gagatcgggg
     5221 aagacgagga cgttcgccct tcccgccacg gggcttgcgc ccttcagctt tttcgccgcc
     5281 actgccggca ccaaggcggt gtccgcttga agctcgccgt ctatggccag atccggggcg
     5341 cgctcccggg cgatcgcaag ggcgcgggtc actttctcga cgcgcgcgtg ctgcgcgctg
     5401 cccttagtgg agaaggaaag cagcgcaacc cggggttctt cgcgcagcac cgcgcggcag
     5461 gattccgcgg aggcaagcgc gatgtcggcg agcgtctccg ggtccgggtc gacgttcacc
     5521 gcacagtccg cgtagatgaa gccgcgctcg gacgcgcctc cgaaaccggg aacgatcatc
     5581 aggaagaagc tcgaggggtt gcggatgccc ccggtcagcc ccacggtcat caatcccgcc
     5641 tcgatcacgc gcccggtggc gaccgtcgcg ccggcgatca tcgcgtcggc gtcgccggcc
     5701 ttgaccatca tcccggcgtg gaacagcggc ttgcgcacca gccgttcggc gaccttcgcg
     5761 ctcgcactcg gccggccggc cgcataaaga ccggcgtacg cctccagccg gtcgctctct
     5821 accggatcga gcaaggtgat gccgtccagc gacacgttcg ctcgtgccgc cgccgcggcg
     5881 atctcgccgc cggcgccgag cagaactggg ctcgcgatac cctgatcctt cagccgcctg
     5941 gcggcggcaa cgatacgctc gtcatgcccc tcggggaata ccaagcgctt gcgggctgcg
     6001 cgcgcacgcg tgacgcacgc atcaagtatg gggctcgccg cctgccgttc accgctcacc
     6061 gcttaccgct cacccaagga gtttgtcgga ggaagttcga gaaactccta gcgccgctgg
     6121 aattgcactt tcccgaacat gatctcccac gatttgtcgc tgtgcggcgc ctggttggcg
     6181 gcctgcactt cggcgagcac cttcaccccg cgctgcaccg cgggccgtgt gctgatctcg
     6241 tcgaaccagc gcttcacgtt gggatactcg gagagatcga cgccgcggtt ctctgcgccc
     6301 ctcatccacg ggaaaatcgc gatgtccgag atggaataat cgtcgcccgc cgtatagcgc
     6361 gcctcgccca atcggtgatc gagcacgcgc gtcagccggt tcgcctcgtt ggtgtaacgg
     6421 tcgatcgcgt actggatctt ttccttcgag tagcggcgga agtggttggc ctggccgaac
     6481 atcggcccga tgccgcccat ctgccacatc agccactgca cgcaggtcca gcgcgcgtgc
     6541 atgtccgccg gcaggagctt gccggtcttg gaggcgaggt agagcaggat cgccccgctc
     6601 tcgaacaggg agatcggcct gccgaccggt ccttcgtgat ccacgatcgc cgggatgcgg
     6661 ttgttggggc tgatcttcag gaactcgggc ctgaactggt cgccttcgcg gatgttgacg
     6721 ccatgcaccc ggtactgaag cccggtctcc tcgagcatga tgtggacctt gtggccgttg
     6781 ggcgtgggcc agctgtagac gtcgatcatg gaattctccc gaaagagcgt gataggtgat
     6841 gagtgataag tgataggtgt tcagcaaaaa actgtcgttg agcgtaataa gtcaggctgc
     6901 gctcgcgatt caagaaagcg aatcctacca actaagtcgc gagggcgtaa accatgccac
     6961 tgacctcgca aggcattcct gttagtcttg cccctgccat gtcgcgttcc gcatccgctt
     7021 cctggctgct gccgccggac acgctgccgg aagcgagggt gctgctcgcg gcgcgggcgc
     7081 tgcgcgccct cggagacggc tatgtcagcc tgctgctgcc ctattacctg acgttgctgg
     7141 gcttcagcgc gctcgaggtg ggcgtgatcg cgaccgcgac ccttctggga tccggcctga
     7201 tgacgctcgg cgtgggaatc atcgcgcacc gtttcccggg ccgcgacctc ctccggctcg
     7261 cctgcctcat catgatcgcg accggcgtgg cagtggtcct tgtcacggat ttctggccgt
     7321 tgctgctggt cgcgttcctc ggcacgctca acccctcctc gggcgatgtc agcgtgttcc
     7381 tgccgctcga gcattcgcag ctcgcgcgca ccgtcgaggc ccggtcccgc acggcgttgt
     7441 tcgcgcgcta cagcctgatc ggggctctgg tcggggcgct cggcgcgcag gccgccgccc
     7501 tgccccagct cttcagcgcg ctcctcggcg tgccggagaa aagctcgatc caggcaatgt
     7561 tcctgctcta cgggttgttc ggggcggcga gcttcgcgct ctaccagcgg ctgccgaccc
     7621 ggctggaagc ccggcaggag gaaaggcccg tgccgctcgg caaatcgaaa ggggtcgtct
     7681 ataccctcgc ggccttgttc agcctcgacg cgttcgccgg gggtttcgcc gtgcagtccc
     7741 tgctcgcgtt gtggctctac gagcgcttcg ggctgtcgcc ggcggcggca ggcacgatct
     7801 tcttctggac cggcgtgctg acggcgttct cctacccggt ggctgcctgg ctcgccgcgc
     7861 gcatcgggct tgtcaacacc atggtgttca cccacctgcc cgcgaacgtg ttttgcatcc
     7921 tcgccgccct gatgccgacg ctgccgctcg cggtcctgtt cctgtgcctg cgcgcggcgc
     7981 tgtcgtccat ggatgtcccg gcgcgcactt cttacgtcat ggcggtggtt tcgccggccg
     8041 agcggccggc ggcggcgggc atcaccgccg tcccgcgcag cctcgccgcc gcggtaagcc
     8101 cggcgttctc gggatatcta ttgagcctca cccctttcgg ctggccgctg gtgatctgcg
     8161 gcgcgctcaa gatcctctac gacctgacgc tgctcgggat gttcaggaag gtcaagccgc
     8221 ccgaagaacg gtgagcggtg agcggtgagc ggtgagcggt gagcggtgag cggtgagcgg
     8281 tgagcggtga gcaaaattag agcggctttg tccgttcact gttcactgtt cacgccccat
     8341 ccttcaaagg caggcggctc gcccgcagca cgggcgccca cttctcgttt tccgtattga
     8401 tgcgggtggc gaactgctcg agcggcatcg ccgcgggttc gagccccagc gccatgagct
     8461 gcatttgagt cgccggatcg gcgatcgccg tggagattcg ctcacgcagc caggcagtgg
     8521 cgacaggcgg gctgcccgcc ggcgcgaaca ggccgtacca gccctcgaga tcgaattccg
     8581 gcaaacccga ctctgcgagc gtcggcaagc cgggcagccg ctcgaagcgc ctggggctcg
     8641 tgatggcaag cggcctgaag tattcattcg ggaaataagc cagcgccaac ggcagagcgg
     8701 cgaacatcag cgagacctgg cgcgccgcca ggccgttcag ggcggccacc ccgccgttga
     8761 aagagacatg gatgatgttg acatgtgcca tgcggcggaa cagctcgccc gccaggtgtc
     8821 cggtgctgcc gtcacccgag gacccgtagt tgacctctcc cgggcgcggc ctgagccagt
     8881 tgatgagcgc ctgcggggtc tcgaccgtca ggtccgatcg caccagcagc acgaacggca
     8941 tggtggccag cggcgcgacc aggacaaaat cgcgcgccgg atcatattcc acgccgtaaa
     9001 aataattcgg attgatgacc atcgtggcgt tgctggcgag cagcagcgta tgcccgtcag
     9061 cggacgctcg cgcaaccgac atcgctccgc gcgtcgtgat cccgcccggc tggcgcttga
     9121 gcagcaccgg ttgccgggta tctgcgcgga tggcttgcgc cgcggcgcgc gccagcgtgt
     9181 cggagattgc cggcggcgca ttgggcgcca tcaagcgata gctgcgtgac atccgggcgg
     9241 ttccccggat gtcggtcggg ccgctgatcg ggtagggaat gacgatcctg acgggcttgg
     9301 aaggaaacgg ctccgccatc gccgcgctgg caccaaccgc cagcgcggcg ataatgggga
     9361 tcgaaaacga aaatgcgggc ggtccaatca ctaatcacca atcactcatc acgcttttcc
     9421 gccgagctgc agcgacttca gctcgagaaa ctcttcgagc ccgtagcggc cgaactcgcg
     9481 accgtgtccc tactgcttgt agccgccgaa gggagcgagg aggttgaaag gcccgccgtt
     9541 cacctcgacc cttccagcac ggatgcgccg ggccaagcgc cgcgcgcgtt cctcgtcatt
     9601 tgaccacacc gcgcccgcaa ggccgtagat cgtgccattg gcgatgcgga tggcctcttc
     9661 ctcgtcccgg taggcaatga tggaaagcac cggcccgaag atctcctcct gctcgatggt
     9721 catcccgggt ttgacccggc cgaacacggt cggctgcacg aagtagcctt ggtcgagacc
     9781 cggcggcggc tccgcgccac cgaggagcaa ctcggcgcct tcctgaacgc ctttcctgat
     9841 gtagccgcgg acccgctcgc gttgaacgcc ggaaatcagc gggccgagcc gcgtgtcatc
     9901 cttcatcgga tcgcccggcc tgaaggtctt cgccgtttcc accgcgagct gcgcggcttc
     9961 cgcgtagcgc gattcgggga ccagcagccg cgtcaatgcc gtgcaggtct gtccggaatt
    10021 gaggaaacag gaattgaccg tgcccttgac cgcgcgggcg aggtcggcgt catcgagcac
    10081 gatggaggcc gacttgccgc ccagttccag cgcgacgcgc tttacggtcc cggccgcaag
    10141 ctcggagacg cgccgcccgg cgcgcgtgga gccggtgaac gaaaccatgt cgatctccgg
    10201 gtgccgcgcg atcgcctcgc ccacgacggg accggtgccg cacaccaggt tgaacacgcc
    10261 gcgcggcagg ttcgaagcat ggatcacctc ggcgaggatg aaagccgtaa gcggggcgat
    10321 ctcgctcggt ttcagcacga tggtgcagcc ggccgcaagc gccggcgcga tcttggccgc
    10381 gacttggtgc agcgggtaat tccacggcgt gatcgcgccc accacgccca ccggttcctt
    10441 gaccaccacc gagttgccga ctttctcctc gaacgggaac tcgcgcacga gccgcgcgta
    10501 gctcgccatc acctgcggcg gcagcgcggc ctgcacgcgc tgcgaaagct tgaatggcat
    10561 gccgacttcg gcggtgatgg tgcgtgcgat ctcgtccgcg cgcgcggcga ggccgtccgc
    10621 gatgtcctgc aggaaggccg cgcgtcgctc gaccggcagc gcggcccacg gctcgaacgc
    10681 cgcccgcgcc gcccgcacgg ccttccccac gtcctgcacc gtgcccgccg gcacccgcgc
    10741 gatgacttct tccgtcgcgg cattgcgaac ctcgagcaaa tccgcggact tcgacggcac
    10801 ccactcgccg ccgatataga gcttgtcgtg gattttcatt tggacaccca ccgccaccga
    10861 aaaccgattg ccgtatctta tcccaacgcc gtcggcccat tcttgcgttg agcggcgggc
    10921 tcattcacac tccgcaaccg gaggactcta tgccgattcg cccagcccga aaactgcatg
    10981 cctttgtacc agcaccttta ctggtcgcgg tcattgcctc ggcaggacct gttgccgtcg
    11041 cagcttccct ggacgttggt cagtacccgg cgcggccagt gcgcatcgtg atcggtttca
    11101 cacccggcgg cgttcccgac atcaccgcgc gcaccatcgc cgcaaagctg accgagcggt
    11161 ggagacagca ggtgatcgtg gataaccgga ccggcgcggg cggcaccatc gccgcacaga
    11221 tcgtggcgag cgccaacccg gacggctaca ccctgctctc ggtgtccaac gcgcattcgg
    11281 tggcgcccgc gatctacgcc aggctgccct acgacacgct caaggatttc gccggcatca
    11341 ccatgaccgc caacggtccc gccttgctgc tcgtctcgcc ctcgatcggc gtcaaatcag
    11401 cgaaggacct gatcgcgctc gccagggcca ggcccggcca gctcaacttc tcctccgccg
    11461 gggtcggcag cggcacccat ttcgggggcg agctgttcaa gagccaggcc ggaatcgacg
    11521 tggtgcacat tcccttcaag ggcatccccg aggcgatcac cgagaccatg accgggcgcg
    11581 tccacttctt tctctcgccg cttgcgagcg cgatcggcct ggtgaaggaa ggcaaggtgc
    11641 ccgcggtcgg cgtcaccagc ctcaagcgca ttccgcaact gccggacatc ccgactgtct
    11701 ccgaatcagg cctgcccggc ttccgatggg attactggta cgggatgctc gccccggcga
    11761 agatcccgcg gcccctgctc aaccagctca accaggagat caccgacatt ctcaaccagc
    11821 cggacgtgcg cagccgctgg accgcgctcg gcgcggagcc ggcgcccacc acgtcgcagg
    11881 cgttcgacaa gttcatcgcc gaggacatcg cgaccctcac gaagatcgcc cgcgcggcga
    11941 atatcaaggc caactagcgc aacgccctgc cgtccctcgg cccgtcccgg aacgcgcggg
    12001 agcgcgcgat ccgccgccgc gtgtttgata ttgatcaata tccgggccgg ctgccgcccc
    12061 cataatccgc ctcatcgttc tcgaggcgag gcgctgcagc gcatcgaatc ccctcacatg
    12121 tccgtcacgg acctgcccgg ctggaccctg aagggccggc gcctgcttcc catcgtgcaa
    12181 ggcggcatgg gcatcggcgt ctcggcgcat cgtctcgccg gcagcgtggc cagactcggt
    12241 gcgttgggca ccatctccag cgtggaattg cggcatcacc atcccgatct cctcgggctg
    12301 gccaaggaaa cgggcgacac ggatgccctc gaccggatga acctgaccgc gctcgaccgg
    12361 gaaatccggc aggcgctcga gatcgcccag ggcgcgggcg ccgtcgccgt caacgtgatg
    12421 aaggccgtgg cgctgcacgc cgcctacgtg cgccagagct gcgagagcgg cgcccacgcg
    12481 atcgtgatgg gcgccggcct gccgctcgac ctgccggaca tgaccaagga cttcccggac
    12541 gtcgcgctga tgccgatact ctcggatgcg cgcggcaccg gcatcgtcgt caagaaatgg
    12601 atgcgtaaaa accgcctgcc cgatgccatc gtgatcgagc acccgcgcta cgccggcggc
    12661 catctcggcg cgacacgatc cgaggagatc cgcgacctgc gcttcgattt cgagcgcgtg
    12721 gtggaaggga tccacgggtt gttgcgcgat ctcgggatcg agcgcgagcg cattcccctg
    12781 gtcctcgccg gcggcatcaa cacgcacgat aaagtacgtg cactgctcgc gctcggcgcc
    12841 tccgccgtgc agatcggcac gccgtttgcc gtgacggtcg agggcgacgc gcatcccgac
    12901 ttcaagcggg tgctcgccga ggcacggccc gaggatatcg tcgaattcat gagcgtcgcg
    12961 ggcctgccgg cgcgcgcggt gctcacgcct tggctgcgcc attacctcca gcgggaaaaa
    13021 gcgctgcagt cgcacgccaa ggccgacccg cgccgctgcg tgcccaagac caagtgcctg
    13081 acggtgtgcg gcctgcgcga cggcatcgcc agcatcgggc aattttgcat cgatatgcgc
    13141 ctggcttacg cgctcaaggg cgacatcaag aaggggttgt tcttccgcgg ctcggaaagt
    13201 cttcccttcg gccaggcgat ccgccccgtg acggagctga tcgaatactt gctcaccggc
    13261 cgcatgccgg agtttgcctg attatgggac gtcgtgctcg cgcacctgct ccaccatgtg
    13321 gtcgagctcc cgctcgaccg actgcttgat ttcctcgtcg tccgggtagc agagcagcca
    13381 gagatcggga aaatcgccgg tgacgcgctt gcggcggccg agcacgagat ggcgacgcac
    13441 gtcttccacc tcgcgcttca gcatgaattc ggtgccgtgc tcccagtaga tcgagagcgt
    13501 gtccaccttc aggctcgagc gcagcaggcg caccacgaac tcgaagcgga ccgaatgcat
    13561 ccccatcgag cgcagttgca cgatacggtg cgaaggaagg ggttccccgg ccttcagtgc
    13621 cttgatctcc tcttgccgtg atgccatgac caccgccatt tttgtggttg ttggccgggc
    13681 gtagcgtagc cgctgtggag gagcctgaca aatctaacaa aacgatagat ttttcgcggt
    13741 tccggcatgt gggaatcggg gtccggccgg ctcacgtcag ggaccagcgt gtctggaatg
    13801 gtccaagacg tcattgccgc gtaacgcggc gcggggacag caatcagcgt tcgccctcgg
    13861 gcagcggctc gcgtccttcc ccctgcttcc agcgcagcca catgcgcagc cggcggaaat
    13921 cccgcggatc cacgttgtcc ggcatgagga tcacacgccg ggttttccat cgtccctgcg
    13981 ggcggagatt gacgatcgtg aggcggggag aaacatagct cgagcccagc acttcgctgt
    14041 ccacccaatc cccgcgccgg gtctggaacg cgatgccgcc ggtgtccctg agctccagcg
    14101 ccacgatcga atgcgcggca tgcagcgccg cgtcccgtgc catgaagaag atgaggctca
    14161 cggcgaccgc cagcgtgaac accgccttgc ctaccacggg gatcggcacg atccacagca
    14221 gcgctgccgc ggcgatatgc gtggcggcga tcgccatggc aaggcgtacc gaagaggtga
    14281 tggagatccg ttcgacaggc atgttatggc agatgcttca gcaaggtcct cctgaaacgc
    14341 cgggcgattt tcctgaagcc gaccaggttc gggtgcagct cgttcgccca gtcggatttt
    14401 ttaagtgtgc cggcggtcct gacgaactcg aaaccggggc ccttgagtcc ggagagcatc
    14461 ttcgcgaagc cgtcgatgaa ctcggcgacg atgtcctcgc ggaattgttt cggcaccctg
    14521 gccagctcca tgtacggatc gagccaggcc atccacagca gcttcttgcc gggcacggcg
    14581 taatcgtagt tgtgcgtgac gataacggcc ccgggccggt acatgtcgcg aagggtcacc
    14641 aagtcgcggt aggcgtgttc gatctcggcc agccgccggc cgggctggcc gtccctgaag
    14701 cattcccggt ggttcgccgc gccgctgcag tcgcgacgca gcagcttcag caggttgtcc
    14761 gcggcgatgt cgttaccgcc accgctgaac agcagcatcc ggatggtcga atacgccttg
    14821 agcgtctcct ggagcaccca gcggctgcgg ccgtccacca tcttgatcgc ctcgtcgccg
    14881 ggatacgcga ggcagaggat ggtgtagcgg tccttccaga tgtcacggtt gaagcggtct
    14941 atgaggttgc cgttgccggg gatccagtag gaaaaccacg agtcgccctc cgccagcacg
    15001 tccggggcgc gatagtcttc ctccatgagc tcctcgttcc aataaacggt catatcagca
    15061 ggacgacctg cagctgtcga aatcggcgcg cgcgcggctc acgcactccc cgtgccagcg
    15121 ggtgttccgg tagtaggctg aatccttgtc ctcgaacaga aggttgcaat tcccgatcct
    15181 cgcctgcagc cgcctcttgc aatcgtccac gcagttcgcg gcgacagcag gaccgggtgc
    15241 ggtcaccagc cccaaccaca acaacattac cgttatctgc agccttttgc ggcgcatgtg
    15301 tagcggtttg cccaccacca ggcggttacg acgttctgct tccagatggg cagcccttcg
    15361 aaccaatcgc ggtcggcatg gcattgcgcg cagggatccg catcagaagg gtccgcacgg
    15421 atgccgtcgc gggaatacat gagcgcgccg aggccgtagg aaatggccaa cagagcggcg
    15481 acgccgagca tgagcacgcc gattatcacc aacaagacca tcaggtattg catatcgtgt
    15541 ttctcctttc ccggagtaac ggagtgacac gccggagacc acaggcgccg gcattgtttt
    15601 cctgccgccg gctgccagcc cataactcta ccgctcgtgg ccgtctttgt gtgtgatctt
    15661 ttgcacagga gcaagccgat atccaggcac gctgtggttg ccgatgcgga tcgcggctcg
    15721 aatcagttcc gatcagcgct cgatagaatg cgcagggaaa gccggccgtc gctcaaaccc
    15781 ggtcaacgcg gagccggccg cgccgcctcc cgctcgagcc agaccgtgac ctcgtccagc
    15841 agcgcggtca gcgcgcggtt gaacgctgca accgcggaag acgcgccctc gccgccggcg
    15901 ggcgcgctct gcgagaacgc gcgccgcgcg aggattgcgc gcctggcatg gtcgatgagc
    15961 tccgcggaca gttcgatccg ggcgctgccg ggatccgtgc cggcgtcctg gtagaactct
    16021 tcgagccgcg tgttgaggac gaggtcgcct gtaacgccgg aagtcgtcga ggtcaccgac
    16081 ttgaaaccgc cgcgcgcgtc gagccggcgc atcagaagct ccgagaaccg ccgtcccggc
    16141 cgctcggtcc aggccgcgaa ctgatagtgg gcgcgctggc ccggcgcccg gctgaaggcc
    16201 aggctctggg tgtcgtagaa cgggctgacg gacgtcgggt tgacgagcag cacgcggtcg
    16261 attgccgtgg cgcgcttcga cgcggccgtc ttcgccaggt cgtcgagaac gtagtattcg
    16321 cgcggcggca gattaccggg cgcgaggcag cccgcaagcc ccgttccgag cgcacacagc
    16381 aggaaccacg caaaattccg cgcccccatg cgtgcatgga tcgctcggat cggggcaagc
    16441 agggcggcgg tggcggcgcg cctcatcgga gcctctcccc ggggccgagt tgcgccgggc
    16501 tcggcccgaa gatcagggcg cgcgggtcct gcaggcggtc tgcggcgcgg gaaaggatct
    16561 cggcgct
//
LOCUS       DBNGWZ010000528         3534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3472413, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000528 DBNGWZ010000000
VERSION     DBNGWZ010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3472413"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /gene="der"
                     /locus_tag="AC16CX_16420"
     CDS             complement(<1..936)
                     /gene="der"
                     /locus_tag="AC16CX_16420"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3014129.1"
                     /translation="MLPIVAIVGRPNVGKSTLFNRLTRSRDAIVADVPGLTRDRHYGR
                     GRVGAKPYLVVDTGGFEPVAKEGILHEMTRQARQAVDEADAVLFVVDARAGLTAQDRM
                     IAEELRRYGRRVHLVVNKAEGLEPAVAGAEFHALGLGDPLPVSAAHGDYVADLIELVL
                     KDAPASVESSRAEGRHPRIAIVGRPNVGKSTLVNALLGEERVIVFDQPGTTRDSIEVE
                     FGRSGRSYTLIDTAGLRRRGRVSETPEKFSVLKALQAIEDANVAILVLDARQDISEQD
                     AHVAGFILEAGRALVVAVNKWDRLPQQERELVKRAL"
     gene            complement(992..2155)
                     /gene="bamB"
                     /locus_tag="AC16CX_16425"
                     /gene_synonym="yfgL"
     CDS             complement(992..2155)
                     /gene="bamB"
                     /locus_tag="AC16CX_16425"
                     /gene_synonym="yfgL"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HIR3014130.1"
                     /translation="MIRQTGSILLLAALALLAGCQTLSDAYDRAFGSRPAQKPAELVP
                     IVSKAALRVLWQGNVGPAEKNVYFPAKSGNIVYAVGAAGGVTGFDAASGAAVARIEAG
                     QRVSGGVGAGSGVVLLGTPKGEVLAFDHDGKALWKAQLAGEVLAPPEAQDGTVVARAG
                     DGRVYGLDVASGRQKWVYQRSTPALSVRTHAGLVVERGAVFVGFPGGRLVGLTLANGS
                     VGWEAVVALPRGATELERVADITSLPVVDGPRVCAAAYQGRVACFDGSRGTLIWARDV
                     SSIAGLTVDERYLYVTDDRNAVLALDKAGGASIWRQDKLAGRNVSAPLAVGKFVVVGD
                     LEGYVHLLSSEDGSFAARITTDGSAISVPPITLDRSSFLVQTRNGGVFAISVQ"
     gene            complement(2176..2823)
                     /locus_tag="AC16CX_16430"
     CDS             complement(2176..2823)
                     /locus_tag="AC16CX_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR3014131.1"
                     /translation="MAYDLEEQEQLSAIKTWWNEYGNFVVAGAVAAALAVAAFQGWRY
                     YRSSQAVAAAVLYEQLDQAERAGDLKKAREIAGQITGKYGATAYGVFAALSAARASFD
                     TGDLAGAKSQLQWVIDKAKEDEIRDIARLRLAGVLLDEKNYAEALKLVEAKPVESMTG
                     LYADLRGDILFTQGKNAEARSAYQLALDKSEAGSPYRAAIQLKLDALGEVASAAK"
     gene            complement(2927..>3534)
                     /locus_tag="AC16CX_16435"
     CDS             complement(2927..>3534)
                     /locus_tag="AC16CX_16435"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR3014132.1"
                     /translation="IEQAPKLADDLDETSLKDFEELQSLLHDADVGFRINPRLVRGLD
                     YYNGAVYEWVSERLGAQNAVCAGGRYDGLVEQIGGKPTPACGFALGVERLLVLVKEGR
                     GVAPRPLDVYLVRQGERADRYAASVAERLRDGGLSVVLNCGGGSFKSQMRKADASGAR
                     FAVIVGDDEAEAGVISVKPLREAAQQVRASITEAIDLIKKP"
BASE COUNT          578 a         1292 c         1048 g          616 t
ORIGIN      
        1 gagcgcgcgc ttcacgagct cgcgctcctg ctggggaagc cggtcccatt tgttgaccgc
       61 caccaccagc gcccgtcccg cttcgagtat gaaaccggcg acgtgtgcgt cctgctccga
      121 gatgtcctgg cgtgcgtcga gcaccagaat cgcgacgttg gcgtcctcga tcgcctgcag
      181 cgccttcaac accgagaact tctccggcgt ctccgacacg cgaccgcgcc ggcgcaatcc
      241 cgccgtgtcg atcagcgtgt aactgcgccc gctgcgcccg aactcgacct cgatcgaatc
      301 ccgtgtcgtg ccgggctggt cgaacacgat gacgcgttct tctccgagca gcgcgttgac
      361 cagcgtggac ttgcccacat tgggccggcc aacgatggcg atcctgggat gacggccctc
      421 cgcccgcgac gattccaccg atgccggggc gtccttcagc acgagctcga tcaaatcggc
      481 gacgtaatcg ccgtgcgccg ctgaaaccgg caggggatcg cccagcccta gggcgtggaa
      541 ttccgccccc gcgaccgccg gctccaatcc ctcggccttg ttgaccacca gatggacgcg
      601 acggccgtag cgccgcaact cctcggcgat catgcgatcc tgggccgtga gaccggcgcg
      661 tgcatcgacg acgaacagga ccgcgtcggc ctcatccacc gcctggcggg cctgacgcgt
      721 catttcgtga agaatgcctt ccttcgccac cggttcgaag ccgccggtgt caaccaccag
      781 atagggcttg gccccgacgc gcccccgccc gtagtggcgg tcgcgcgtca ggcccggcac
      841 gtcggcgacg atcgcgtcgc ggctgcgggt gagccggttg aacagcgtgg acttgccgac
      901 gttcggccgg ccgacgatgg cgacaattgg gagcatgact caaggatgcg ggatacggga
      961 tgcgggacgt cggcgcacta agtttttgcg atcactgcac ggagatcgca aaaactccgc
     1021 cgttgcgggt ctgcacgaga aaactcgacc ggtcgagcgt gatgggcggg accgagatgg
     1081 cgctgccgtc ggtcgtgatg cgcgccgcga aggagccgtc ttcgctcgag agcagatgca
     1141 cgtagccctc gagatcgccg accacgacaa atttcccgac cgccagcggc gcgctcacgt
     1201 tgcgcccggc aagcttgtcc tgccgccata tgctggcgcc acccgccttg tcgagcgcca
     1261 gcaccgcgtt cctgtcgtca gtcacgtaaa gatagcgctc gtccaccgtc agccccgcga
     1321 tgctggagac gtcccgcgcc cagatcaggg tgccgcggga gccgtcaaag cacgccaccc
     1381 gtccctggta cgccgccgcg caaacccgtg gaccatcgac caccggcagg ctggtgatgt
     1441 cggcgacgcg ctccagttcg gttgccccgc gcggcagcgc taccaccgcc tcccagccga
     1501 cgctgccgtt ggcgagcgtc agtccgacta gacgcccgcc gggaaatccc acgaacaccg
     1561 cgccgcgttc caccaccaga ccggcgtgcg tgcgcaccga cagcgccggc gtcgagcgct
     1621 gatacaccca tttctgcctg ccgctggcga cgtccagccc gtaaacacgg ccgtctccgg
     1681 cgcgcgcaac cacggtgcca tcctgcgctt ccggcggcgc cagcacttca cccgcaagct
     1741 gcgccttcca aagcgctttc ccatcgtggt cgaatgcgag cacttcaccc ttgggcgtcc
     1801 caagcagcac cacaccgctt cccgcaccaa cgccgcccga aacgcgctgg cccgcctcga
     1861 tgcgcgcgac ggccgctccg cttgcggcat cgaagccggt gacgccgccc gccgcgccga
     1921 cggcgtagac gatattcccg gatttcgccg gaaagtaaac gtttttttcc gccggcccga
     1981 cgtttccctg ccacaggacc ctgagcgcgg ctttggacac gatcggaacc agctccgccg
     2041 gtttctgcgc cgggcggctg ccgaaggcgc ggtcgtaggc atcggagagg gtctggcagc
     2101 cggccagcaa cgccagcgcc gcgagcaaga gtatcgatcc cgtctgccga atcattcaat
     2161 caccctgtca cttcatcact tggccgctga agcgacttct cccagagcgt cgagcttgag
     2221 ctggatggcg gcacggtagg gactccctgc ctcgctcttg tccagcgcaa gctggtaggc
     2281 gcttcgcgcc tcggcgttct ttccctgcgt gaacaggata tcgcccctca agtcggcgta
     2341 aagcccggtc atggactcca ccggctttgc ctccaccagc ttcagcgcct ccgcgtagtt
     2401 cttctcgtcg agcagcacgc cggcaagcct cagccttgcg atgtcccgta tctcatcctc
     2461 cttcgctttg tcgatcaccc attgcagctg cgacttcgcc ccggcgaggt ctcccgtatc
     2521 gaagctcgcc cgggctgcgc tcaacgccgc gaacacgccg taggccgtgg cgccgtattt
     2581 ccccgtgatc tggcccgcga tctcccgcgc tttcttgaga tcgccggcgc gttcggcctg
     2641 gtcgagctgt tcgtagagca cggccgccgc caccgcctgg ctcgaccgat aatagcgcca
     2701 gccctggaac gcggcgacgg cgagcgccgc ggcgacggcc cccgctacca cgaagttgcc
     2761 gtactcattc caccacgtct tgatcgccga cagctgttcc tgttcttcga gatcgtatgc
     2821 catttcaatt ccctgattgc gtgggttgtg ggtcgtgggt tgtgggtcga atcagaatcg
     2881 attgttcaga tcaacgaagc acgatccacg acccacgatc cacgaatcac ggtttcttga
     2941 tgaggtcgat cgcttccgtg atcgaggcgc ggacctgttg cgcggcctcg cgcagcggtt
     3001 ttacgctgat cacccccgcc tccgcctcat cgtctcccac gatgaccgca aagcgcgcgc
     3061 cgctcgcgtc cgccttcctc atctgtgatt tgaagctgcc gccaccgcaa ttcagcacta
     3121 cggaaagacc cccatcgcgc aggcgttccg cgacactggc ggcgtagcga tcggctcgct
     3181 ctccctgccg cacgagatag acgtcgagcg ggcgtggtgc gacccccctg ccctccttca
     3241 ccagcacgag caggcgctcc acccccagag cgaaaccgca cgcgggtgtg ggcttgccgc
     3301 cgatctgttc gaccaggccg tcgtagcgcc cgcctgcgca caccgcattt tgcgcgccga
     3361 gacgctcgct cacccactcg tagaccgccc cgttgtaata atcgagcccg cgcacgagcc
     3421 gcggattgat ccggaaaccc acgtctgcat cgtgcaggag cgactgcagc tcctcgaaat
     3481 ccttcagcga ggtctcatcg agatcgtcgg ccagcttcgg cgcctgctcg atca
//
LOCUS       DBNGWZ010000529         3032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2337990, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000529 DBNGWZ010000000
VERSION     DBNGWZ010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2337990"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..964)
                     /gene="ttcA"
                     /locus_tag="AC16CX_16440"
                     /pseudo
     CDS             complement(158..964)
                     /gene="ttcA"
                     /locus_tag="AC16CX_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            complement(1091..1843)
                     /locus_tag="AC16CX_16445"
     CDS             complement(1091..1843)
                     /locus_tag="AC16CX_16445"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HIR3014133.1"
                     /translation="MEETLLGKAVLVTGGAKRVGAAICRRLHAAGASLLVHYRSSSIE
                     ARALQVELNAGRADSVALAQADLLKIANCSQIVKAALKHFGRLDALVNNASSFFPTAI
                     GEITEQAWDDLIGTNLKAPLFLAQAAAAELKKNHGCIVNLIDVHAEFPMRNHAVYTAA
                     KGGLAALTRALARDLGPEVRVNGIAPGTILWPDDETWRDEVARQRIINQSALKRIGEP
                     DDIARAVQFLLADAPYITGQILTVDGGRSISL"
     gene            1964..>3032
                     /locus_tag="AC16CX_16450"
     CDS             1964..>3032
                     /locus_tag="AC16CX_16450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014134.1"
                     /translation="MSAQLARFIAAEIERGGGWLSFARYMELALYAPGLGYYMAGARK
                     LGRDGDFVTAPEISRVFGRTLARQVQQLAGLGLTDILEIGAGSGALAADLLLELERLG
                     STPARYWILELSPDLRDRSRDTLAERAPHLLERVAWLNQLPPTFTGVVIGNEVLDAMP
                     VHVFQREGARIVELGVEYEGGNFRWASRPAKSPSVPPDPSCFPIDGYRSEVHLVARAF
                     IQRLGEVLTHGVAMFLDYGFPRREYYHPQRGSGTLMCHYRHRAHDDPFFLPGLQDITT
                     HIDFSAIARAGGEAGLELLGYTNQAQFLVNCGITDLLGETPAGDAAAYAPLAAQAQKL
                     LSPAEMGELFKVIALGRGVTQP"
BASE COUNT          476 a         1001 c          990 g          565 t
ORIGIN      
        1 atcgcggcca tgcgcgcgag gacgtcgggg gcccggacca cttcgttcac cgcctggctg
       61 cgccgcttga cgacttcgcg gtcgtgcctc gacaccaaat gaaattaagt cacacgtctg
      121 ctaggacagg cccgccccgg ctcgggggca accggtcgaa gtcgaacgca tctctgtcga
      181 agaggtggga cacccgcacg tgcttcaggc tgttgagcat ggtttcgacc cgacccggat
      241 ggcgcttttc ccactcgcgg atcaacgctt tcatctcgtt gcgcttgagg ctctcctgag
      301 agccgcacag gttgcagggg atgatcggaa actgtcttaa ctccgcgtac gccgcgaggt
      361 cgcgctcctc gcagtaggca agcgggcgga tcacgacgtg ccggccgtcg tcggagcgca
      421 gcttcggcgg catggccttc aactggccgg tgtagaagag attcagcaga aaggtctcaa
      481 ggatgtcgtc gcggtgatgc cccagcgcga tcctggttgc gccgagctcc cccgccacgc
      541 ggtagagcac gccgcgccgc agccgcgagc agagcgagca catggtcctg ccttcaggta
      601 tcaggcgctt taccgtgcta taggtgtcct gcgtttcgat ccggaacggc acgccgaggc
      661 gcgcgaggta atcgggcagc acgtgcgccg ggaaaccggg ctgcttctgg tcgaggttca
      721 ccgccacgag ctcgaagcgg atcggcgcgt gttgccgcag gtagagcagg acgtcgagca
      781 gggcgtagct gtccttgccg ccggagacgc acaccatcac cttgtcgccg tcggcgatca
      841 tgccgtagtc ggcgatcgcc tcgcccgcga gccggcgcag ccgcttcacg agcttgttcg
      901 cctcgtacga ttccttgcgc tccttcgtgc tgcgcggctg aattcgaatt gcggtcgttt
      961 ccatatcgtc tttcccatga tgcgtgacga gtgataggtg ataagtgata agggtcgcaa
     1021 cccgcgcact gcgcggggaa aactcctatc acctgtcact tatcacttat cacgtttttc
     1081 cagcaaagcg tcaaaggctg atgctgcgcc ccccgtccac cgtgagtatc tgcccggtga
     1141 tatagggtgc atccgcgagc aggaattgta ccgcccgcgc gatgtcgtcg ggctcgccga
     1201 tgcgcttcag ggcgctctgg ttgatgatgc gctggcgcgc cacctcgtcg cgccaggtct
     1261 cgtcgtccgg ccacaggatg gtcccgggcg cgatgccgtt cacgcgcacc tcggggccga
     1321 ggtcgcgtgc gagcgcgcgt gtgagcgcgg cgagcccgcc cttggcggcg gtatagaccg
     1381 cgtggttcct catcgggaat tccgcgtgga cgtcgatcag gttgacgatg caaccgtggt
     1441 tcttcttcag ctccgccgcc gccgcctggg cgaggaacag cggcgccttg agattggtgc
     1501 cgatgagatc gtcccaggcc tgttcggtga tctcgccgat cgcggtggga aagaagctcg
     1561 acgcgttgtt gacgagcgcg tcgagccggc cgaagtgctt cagcgccgcc ttgacgatct
     1621 gagagcagtt cgcgatcttg agcagatcgg cctgcgccag cgcaaccgaa tcggcgcggc
     1681 cggcattgag ctccacctgc agcgcgcgcg cctcgatgct ggaggagcgg tagtgcacga
     1741 gcaggctcgc gcccgcggcg tgcagccgcc ggcagatggc ggcgccgacc cgcttcgcgc
     1801 cgccggtgac cagcaccgct ttgcccagca acgtttcttc cattccgcgt cctatgccgc
     1861 cgcttcgagc gtgtagaatt gtattccatt ccccgcgatt tcccgcttgg ccacgccttc
     1921 gcatccagac caactccctg cgccgctggc cgaggcggcc gccatgagtg cgcagctcgc
     1981 ccggttcatc gccgcggaaa tcgagcgcgg tggcggctgg ctctccttcg cgcgctacat
     2041 ggagctcgcg ctctacgcgc ccggccttgg ttattacatg gccggcgcgc gcaagctcgg
     2101 gcgtgacggc gatttcgtca cggcgcccga gatttcgcgc gtgttcggcc gcacgctggc
     2161 acggcaggtg cagcagctcg cgggactcgg gctcaccgac atcctggaaa tcggcgctgg
     2221 cagcggcgcg ctcgccgccg atctcctgct cgagcttgag cggctgggca gcacgccggc
     2281 gcgttactgg atactggaac tctctcccga tctacgcgac cgcagccgcg acacgctcgc
     2341 ggagcgcgcg ccgcatctgc tcgagcgcgt ggcgtggctg aaccagctgc cgccgacttt
     2401 caccggcgtc gtgatcggca acgaggtgct cgatgccatg ccggtgcacg tgttccagcg
     2461 cgaaggcgcg cgcatcgtgg aacttggcgt ggagtatgaa ggcggcaatt tcagatgggc
     2521 ctcacgccca gccaaatctc catccgtgcc gccagatccg tcgtgtttcc cgattgacgg
     2581 gtatcgctcc gaagtgcatc tcgtggcgcg cgcctttata cagagactgg gtgaggtgtt
     2641 gacgcacggt gtggcgatgt ttctcgacta cggtttcccg cgccgggagt attatcaccc
     2701 gcagcgcgga agcggcacat tgatgtgcca ttaccggcac cgcgcacacg acgacccgtt
     2761 ttttctgccg ggcctgcagg acatcaccac gcacatcgat ttctccgcca tcgcgcgggc
     2821 gggcggggaa gcggggctgg aacttctcgg ctacaccaac caggcgcagt tcctggtgaa
     2881 ctgcggcatc accgatctgc tcggcgaaac cccggcaggc gacgccgcgg cctatgcgcc
     2941 gctcgcggcg caggcgcaga aacttctctc tcccgccgag atgggcgagc tgttcaaggt
     3001 gatcgcgctg ggccgcggcg tgacccagcc gc
//
LOCUS       DBNGWZ010000530         3655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2088264, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000530 DBNGWZ010000000
VERSION     DBNGWZ010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2088264"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="AC16CX_16455"
     CDS             <1..885
                     /locus_tag="AC16CX_16455"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3014135.1"
                     /translation="LNSFSDPYKTYRSITKPGAKVITISMQDVYHKSNYQDFQRYMPA
                     DIAIGGDVEASLPDLIEAVKRATTGERKAEFAERAKAVAKMHRNMKARDKERMALGWD
                     ASPVSTARLAAEMWNVIKNEKWALPVSVRIPWARRLWPVTEHYQMLGGNGAAGVGGYA
                     PIAVGAALANKDKGILTVNFQSDGDLMYAPGLLWTCAHHKIPILMLMHNNRCYHQEIM
                     HVQRMAALHNRPQHTARIGTEITDPDIDFARLAQGMGVWAEGPITDPAKVGPALKRAL
                     AEVKNGRPALVDVVCQGR"
     gene            complement(989..1384)
                     /locus_tag="AC16CX_16460"
     CDS             complement(989..1384)
                     /locus_tag="AC16CX_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014136.1"
                     /translation="MTKAEVGDMWKWILVAAGAFAVAGGAIAQTAQSLGAPPGNPERG
                     KKLYMEQLCYTCHGTVGQGGERGAGPKIYPNPFPFPAFVAQVRKPRQVMPPYTEKYVS
                     DQDLADMYRYLFSTKPSPAAKDIPLLKDF"
     gene            complement(1405..3144)
                     /locus_tag="AC16CX_16465"
     CDS             complement(1405..3144)
                     /locus_tag="AC16CX_16465"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HIR3014137.1"
                     /translation="MATKKSKSGMRKGLTAYGDEEFSLFLRKAFIKAMGYSDDALERP
                     IIGITNTYSGFNACHRTVPELIEAVKRGVMLAGGLPVEFPTITLHESFAYPTSMYLRN
                     LMALDAEEMIRAQPVDAVVLIGGCDKTVPALLMGAASANVPAILMVTGPMITGDHKGE
                     RLGACTDCRRFWARFRASEIDEREINAVNAQLCPSAGTCMVMGTASTMALCSEALGMM
                     LPGGACIPAVMAERMRNAEATGARAVGLAKERLTPDKIMTPAAFENALRILLAIGGST
                     NGIVHLSAIAGRLGITVDLDAFDRMGRKTPVLVDLKPTGQHYMEDLYKAGGTATILRE
                     LKPLLDLDALTVTGRTLGEDIASAPPPFPQKVVRPLRNPIFKEGGIAFLRGNLAPDGA
                     IIKQSAMDPKLMRHEGKAVVFESLEDLAKRIDDPKLDVAASDVLVLQNAGPKGAPGMP
                     EAGYIPIPKKLAQQGVKDMVRISDARMSGTAFGSIVLHVTPESAAGGPLALVRNGDRI
                     RLDVSARRLELKVSPVELAKRRKGWRAARPRPEDRRGYRKLFLESVTQADRGCDFDFC
                     APDAKIRVPRKKK"
     gene            complement(3402..>3655)
                     /locus_tag="AC16CX_16470"
     CDS             complement(3402..>3655)
                     /locus_tag="AC16CX_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4870 family protein"
                     /protein_id="HIR3014138.1"
                     /translation="GITFIAAVIVNYVKRRDVEGTWLESHFRWQIRTFWYGLLWSFLG
                     VVLLFVLVGFFVLMASAVWVLYRAIKGWIHLEEGKPMYA"
BASE COUNT          664 a         1219 c         1129 g          643 t
ORIGIN      
        1 ctgaattcct tcagcgatcc ctacaagacc taccggtcga tcaccaagcc gggcgcgaag
       61 gtgatcacga tctcgatgca ggacgtgtat cacaagtcga attaccagga cttccagcgc
      121 tacatgccgg ccgatatcgc gattggcggc gacgtggaag cctcgttgcc cgacctgatc
      181 gaggcggtga agcgagcgac taccggcgag cgcaaggccg agtttgccga gcgcgccaaa
      241 gcggtcgcca aaatgcaccg caacatgaag gcccgcgata aggaaaggat ggcgctcggc
      301 tgggacgcga gcccggtcag caccgcgcgc ctcgcggcgg aaatgtggaa cgtcatcaag
      361 aacgaaaaat gggcattgcc ggtgagcgtc cgcatcccgt gggcgcgtcg cctgtggcca
      421 gtcacggagc actaccagat gctcggcgga aacggggctg ccggggtcgg cggctatgcg
      481 ccgatcgccg tgggtgccgc gctcgccaat aaggataagg ggatcctcac ggtcaacttc
      541 cagtccgacg gcgatctcat gtacgccccg ggattgctgt ggacctgcgc gcatcacaag
      601 atcccgatcc tgatgctgat gcacaacaac cgctgctacc accaggaaat catgcacgtg
      661 cagcgcatgg cggcgctcca caaccgcccg cagcacacgg cgcgcatcgg gacggagatc
      721 accgaccccg acatcgactt cgccaggctc gcgcagggga tgggcgtgtg ggcggaagga
      781 ccgatcaccg acccggcaaa ggtcgggccg gcgctgaagc gcgcgctcgc cgaggtgaag
      841 aacggccgcc ccgcgctggt cgacgtcgtg tgccaggggc gctgagcgca ggcgcactca
      901 gctaaggcgg aagtcggaag gaaaacccta aagccgcttt accctttcgc ctttcgactt
      961 tcgcctttcg ccttgctcaa agacttcatc agaagtcttt gagcagcggt atgtctttcg
     1021 ccgccggcga cggcttggtc gagaacaggt agcggtacat gtcggcgaga tcctggtcgc
     1081 tgacgtattt ctcggtgtac ggcggcatca cctgccgcgg cttgcgcacc tgcgcgacga
     1141 acgccgggaa aggaaaggga ttcggataga tcttcggccc ggcgccgcgc tccccgccct
     1201 gccccaccgt gccgtggcag gtgtagcaga gctgctccat atagagtttc ttgccgcgct
     1261 cgggattgcc gggcggtgcg ccgaggcttt gcgccgtctg cgcgattgcg ccgcccgcca
     1321 ccgcgaatgc tcccgcggca accagaatcc acttccacat gtctccgacc tcggctttcg
     1381 tcaccgcgtc acctcgtcac atcgtcactt tttcttgcgc ggaacgcgta tcttagcgtc
     1441 cggcgcgcag aaatcgaagt cgcagccgcg gtcggcctgc gtgaccgact cgagaaacag
     1501 cttgcgatag ccgcggcgat cttccggacg cggccgggca gcgcgccagc ctttacgccg
     1561 tttcgcgagt tccaccggac tgaccttgag ttccagcctg cgcgccgaaa cgtcgagccg
     1621 gatgcggtcg ccgttcctca ccagcgcgag cggcccgccc gccgccgatt ccggcgtcac
     1681 gtgcagcacg atcgagccga atgccgtccc gctcatgcgc gcatccgaaa tccgcaccat
     1741 gtccttcacg ccctgctgag cgagtttctt cgggatcggg atgtagcctg cttccggcat
     1801 gccgggcgcg ccctttgggc ccgcgttctg cagcaccagc acgtcgctcg ccgcgacgtc
     1861 gagcttgggg tcgtcgatgc gcttcgcgag gtcctcgagc gattcgaaca cgacggcttt
     1921 tccctcgtgg cgcatcagct tcgggtccat cgccgactgc ttgatgattg cgccgtcggg
     1981 cgcgaggttg ccgcgcagga acgcgatgcc gccctccttg aagatcgggt tcctcaaggg
     2041 gcgcaccacc ttctgcggaa aaggcggcgg agccgaagca atgtcttctc cgagcgtcct
     2101 gccggtcacg gtgagcgcgt cgaggtcgag caacggcttc agctccctca ggatggtcgc
     2161 cgtcccgccc gccttgtaga ggtcttccat atagtgttgg cccgtgggct tcagatccac
     2221 gagcacgggg gtcttgcgcc ccatccggtc gaaagcatcg aggtccaccg tgatcccgag
     2281 ccgccccgcg atcgcggaga ggtgcacgat gccgttggtc gagccgccga tcgcgagcag
     2341 gatccggagc gcgttctcga acgccgctgg cgtcatgatc ttgtcaggag tcagtcgctc
     2401 tttcgcaaga ccgaccgcgc gcgcgccggt cgcctcggcg ttgcgcatgc gctcggccat
     2461 caccgcgggg atgcacgccc cgcccggcag catcatgccg agcgcctccg aacagagcgc
     2521 catggtggaa gccgtcccca tcaccatgca ggtgccggcg gaaggacaga gctgggcgtt
     2581 caccgcgttg atctcgcgct cgtcgatctc ggacgcgcga aagcgcgccc agaagcggcg
     2641 gcagtcggtg cacgcgccga gccgctcgcc cttgtggtcg ccggtgatca tcggccccgt
     2701 caccatcagg atcgccggca cgttcgcgct cgccgcgccc atgagcagcg ccggcaccgt
     2761 cttgtcgcag ccgccgatca gcacgaccgc gtccaccggc tgcgcgcgga tcatctcctc
     2821 ggcatccagc gccatgaggt tgcgcagata catgctggtc gggtacgcga acgactcgtg
     2881 caaggtgatc gtgggaaact cgaccggcag cccccccgcg agcatgaccc cgcgcttcac
     2941 cgcctcgatc agctcgggca cggtgcggtg gcaggcgttg aaaccactat aggtgttcgt
     3001 tattccgatg atggggcgtt cgagcgcatc atcggaatag cccatcgcct tgatgaaggc
     3061 cttgcgcagg aacagcgaga attcctcgtc gccgtaggcg gtgaggccct ttctcattcc
     3121 actcttggat tttttcgtcg ccatcgaaac gttattcctt ggagtttcgg actgcgtgac
     3181 tcgtttctcg attctcgtta ctcgccagtc gataagacta tccggagttc gctattcgcg
     3241 cttcgagtaa cgagaaacga acaacgattc cagagccggc acaatagcgg atgcccgccg
     3301 ccatgcgcaa gtagcccgca aatgcccatg gccgcccgtc actgcgtcac ctcgtcaatt
     3361 cgtcactcga cactcgacac tcatcactca tcaccacgcc gtcaggcgta catgggcttg
     3421 ccttcctcca aatggatcca gcccttgatc gcgcgataga ggacccatac cgccgacgcc
     3481 atcaggacga agaagccgac gaggacgaac agcaggacga cgccgagaaa ggaccaaagc
     3541 agcccgtacc agaaggtgcg gatctgccag cggaagtgcg actcgagcca ggtgccttca
     3601 acgtccctgc gtttcacgta attgacgatc accgcggcga tgaaggtgat cccga
//
LOCUS       DBNGWZ010000531         7524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1960677, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000531 DBNGWZ010000000
VERSION     DBNGWZ010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1960677"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16CX_16475"
     CDS             complement(<1..299)
                     /locus_tag="AC16CX_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2201 family putative metallopeptidase"
                     /protein_id="HIR3014139.1"
                     /translation="MRDIRTKLGAARTRLILEKPFIGALVMHLPLVAADPRWCGTAAT
                     DARAFYYNPEYIAGLTLQQTQFVLAHEAMHCALGHFARRQHRTRRRWDVSCDHAV"
     gene            complement(305..1390)
                     /locus_tag="AC16CX_16480"
     CDS             complement(305..1390)
                     /locus_tag="AC16CX_16480"
                     /inference="COORDINATES: protein motif:HMM:NF019348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3014140.1"
                     /translation="MIGSPTLMRPSHILQILEREFTSTASAAHTPVMLWGAPGVGKSQ
                     IIAQIAGKHGVPLIDVRLSQMEPTDLRGIPFRSGERVEWSVPALLPDEKRHGARGILF
                     LDEITSAPPTVTAAAYQLILDRRLGEYRAPGGWVIFAAGNRYGDRGVTYVMPTPLANR
                     FTHYEIEPNLDDWVAWAHAAGMDSRVIAFLRFRPDLLFDFNPAQNPVAFPSPRSWEYA
                     HRALAKFDDAPDLLLDGVQACVGPAAGVEFKAFVDNLHELPDIDAIVRGESAQVPRGI
                     DLQYGVAAALVRRAVQVRELPHANLVYGNILKYAKTFPQREMGVMLVTDLHRSIGKPL
                     ITVPEFAEWAGSITDLMLYDLKLASTS"
     gene            1476..2018
                     /locus_tag="AC16CX_16485"
     CDS             1476..2018
                     /locus_tag="AC16CX_16485"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3014141.1"
                     /translation="MKFCSHCGAPVTLRVPPGDTLPRHVCDSCQAIHYQNPRMVVGCI
                     AEWEDRILLCRRGIEPRHGLWTVPAGYMENGETTFQGAIRETLEEAHARVEIGPLYAL
                     YNIPHISQVYILFRARLLDADVRPGAETLEVKLFTEGEIPWDRIAFASVRNTLTHYYE
                     DRRRGEYQFHMGTIEQPKKP"
     gene            2102..3295
                     /locus_tag="AC16CX_16490"
     CDS             2102..3295
                     /locus_tag="AC16CX_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR3014142.1"
                     /translation="MTAAIPTTKPAFQWDDPLLLEEQLTEEERMVRDTARAYCQEKLM
                     PRVLEANRHEKFDREILLEMGKLGFLGSTLSGYGCAGVNHVCYGLVAREVERVDSGYR
                     SALSVQSSLVMHPIHEFGTEAQRKKYLPKLASGEWIGCFGLTEPDHGSDPGGMVTRAK
                     KVSSGFVLQGAKMWISNAPYADVFLIWAKDESGEIRGYLLEKGMKGLSTSRIEGKFSL
                     RVSPTGEVVMEQVFVPEENLLPGAAGLKGPFSCLNKARYGIAWGALGAAEFCWHAARN
                     YVMERRQFGRPLAANQLIQLKLANMQTEITLGLQGALRLGRLMDEGRATLEMISLLKR
                     NACGKALDIARMSRDMHGGNGIADEFHVIRHVMNLEAVNTYEGTHDIHALILGRAQTG
                     IPAFS"
     gene            complement(3418..4158)
                     /locus_tag="AC16CX_16495"
     CDS             complement(3418..4158)
                     /locus_tag="AC16CX_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR3014143.1"
                     /translation="MEWLLDPHAWLALATLTVLEIVLGVDNIIFITILCGRLPEHERP
                     KARTIGLALAMLTRLALLLFLTWIMTLTAPLFALFGKEISGRDLILIGGGLFLLWKSV
                     HEIHDSLEGEEPDSRPVAAAATFRSVVVQIAIIDIVFSLDSVITAVGMVDQLVIMVVA
                     IIAAVGVMLFAAGPIGAFVDRHPTIKMLALSFLILVGVALIGEGWDLHIPKGYIYFAM
                     AFAVAVEMLNIRIRARMREPVHLRKHEP"
     gene            4380..5339
                     /locus_tag="AC16CX_16500"
     CDS             4380..5339
                     /locus_tag="AC16CX_16500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014144.1"
                     /translation="MRHASLACGMLLIASTAVAQSQIYPSRAVRIIVPFAPAGGTDIL
                     ARSLAQKMGESTGQQFIVDNRAGGNSIIGTDIVAKSTPDGYTLLFTTNIYTINPWLHQ
                     KMPYNAETDFAPITLAGSAPTLLAVHPSLPARTLRELVALAKSKPGQLTMSAAGSGTP
                     SHLSGEFLKQTAGIDVLIVQYKGTGASLSDLVGGQVAMSFGSLPGLAPLVKSGKLRAL
                     AVSGVKRSSSLPEVPTVMETYPEFEVLIWYGLFAPARVQRDIVNRLHAEVVKALANEE
                     ARQRLAVQGFDPGGMTPEQFADTIRRDMSRWRKVIAKASIRVE"
     gene            5625..7445
                     /locus_tag="AC16CX_16505"
     CDS             5625..7445
                     /locus_tag="AC16CX_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014145.1"
                     /translation="MKPTDIGAPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYSDAY
                     MVNWYSNVFPGILGRTCDRPCEPACRRGRVEERNAEKPEPVAICRLKRVAADNKDDIR
                     GRLPKVPKQKNGKRIACIGAGPASLTVARDLLPLGYSVTVFDQDPRTGGMIWSQIPRF
                     RLPLEVIDEEVGYILDLGVEFRGGVRIESMQQLLAEGYDAIFVGCGAPRGRDLDIPGR
                     KEAASNIHIGIDWLSSVSFGHVDKIGKHVIVLGGGNTAMDCCRTSRRLGGEDVKVIVR
                     SGFEEMKASPWEKEDAMHEGIPILNYLVPRAFTHENGRLTGVTFEKVRAEYDAGGRRN
                     LVHTGEPDQHFECDDVLVAVGQENAFPWIERDIGIEFDKHSMPKVDNVTMQSTLPNVL
                     FGGDAAFGPKNIIWAVAHGHEAAISIDKLCQGEDVKERPAPMVNLTSQKMGIHEWSYD
                     NDISLAGRYKVPWRDKKITLRDVKVEVEMGFDPKTGLAEAQRCLNCDVQTVFTGKLCI
                     ECDACVDICPMDCITFTDNGEESELRDRLNAPANNPTQDLYVSDALKTGRIMVKDEDV
                     CLHCGLCAERCPTGAWDMQKFLLDMTHAGPACRSPKRKAA"
BASE COUNT         1465 a         2405 c         2395 g         1259 t
ORIGIN      
        1 ttgacggcgt ggtcacagga aacgtcccaa cgccggcgcg tgcggtgctg gcggcgcgcg
       61 aaatgcccca gagcgcaatg catcgcctcg tgggcgagca cgaactgggt ctgctggagc
      121 gtcagccctg cgatgtactc cggattgtag tagaacgcgc gcgcgtcggt cgccgcggtc
      181 ccgcaccacc gcgggtcggc cgccaccagc ggcaggtgca tcaccaatgc gccgatgaac
      241 ggtttttcca ggatcagccg ggtgcgagcc gcgccgagct tggtccgaat gtcgcgcaag
      301 tcggtcaaga cgtggaagcg agcttcaggt cgtagagcat cagatcggtg atgctgcctg
      361 cccattcggc gaattcgggc accgtgatga gcggtttgcc gatcgagcgg tggagatcgg
      421 tgaccagcat cacccccatc tcgcgctgcg ggaaggtttt cgcgtacttg aggatgttgc
      481 cgtaaaccag gttggcgtgc ggcagctcgc gcacctggac cgcgcgccgc accagcgccg
      541 cagcgacgcc gtactgaagg tctatgccgc gcggcacctg ggcgctctcg ccccgcacga
      601 tggcgtcgat atcgggcagc tcgtgcaggt tgtccacgaa cgccttgaac tccacgcccg
      661 ccgccggccc aacgcacgcc tgcacgccgt cgagcaggag gtcgggagca tcgtcgaact
      721 tggcgagcgc ccggtgggcg tattcccacg agcgcgggct cggaaacgcc accgggttct
      781 gcgcggggtt gaaatcgaac aggaggtcgg ggcggaagcg caggaacgcg atcacgcggg
      841 aatccatgcc cgccgcatgc gcccacgcca cccagtcgtc gagattgggc tcgatttcgt
      901 aatgggtgaa acggttggcg agcggggtcg gcatcacgta tgtcacgccg cggtcgccgt
      961 agcggttgcc cgccgcgaag atcacccagc cgccgggagc gcgatactct ccgaggcggc
     1021 gatcgaggat cagttgatag gcggccgcgg tgacggtggg tggcgcagag gtgatctcat
     1081 cgagaaacag gatgccgcgc gcgccgtggc gcttctcgtc gggcagcagc gccggcaccg
     1141 accattcgac gcgctcgccg ctgcggaacg gaatgccacg gagatcggtg ggctccatct
     1201 gcgacaagcg cacgtcgatc agaggcacgc cgtgcttgcc ggcgatctgc gcgatgatct
     1261 gggatttgcc tactcccggg gcgccccaca gcatcaccgg ggtgtgtgcg gccgaggccg
     1321 tgctcgtaaa ctcgcgttcc agtatctgca gaatgtgcga cggacgcatg agggtcgggc
     1381 taccaatcac gaatcaccag tcactcgcga tagcaacgct atcgctacgc tgagatgtta
     1441 ccgtatagtt atggctcgaa ggaacctgca tcgccatgaa gttctgcagc cactgcggcg
     1501 ccccggtcac gctgcgggtg ccgcccggtg acacgctccc gcgccacgtc tgcgacagct
     1561 gccaggccat ccattaccag aacccgcgaa tggtggtggg gtgcatcgcc gaatgggagg
     1621 accgcatcct gctgtgccgt cgcggcatcg agccgcgcca cgggttgtgg accgttccgg
     1681 cgggatacat ggaaaacggg gaaaccacct tccagggcgc aatccgcgag acgctggagg
     1741 aagcccatgc ccgcgtcgag atcggcccgc tctacgcgct ctacaacatc ccccacatca
     1801 gccaggttta catcctgttc cgcgcgcgtc tgctcgatgc cgacgtgcgg cccggcgccg
     1861 agacgctcga ggtgaagttg ttcacggaag gcgagatacc gtgggaccgg atcgccttcg
     1921 ccagcgtgcg caacacgctc acccactact acgaggaccg caggcggggc gaataccagt
     1981 ttcacatggg aacgatcgag cagcccaaga aaccgtgacg ggtgattggt gactggtgac
     2041 cggagccgtc cggcgagcgg gtccgaggcg taaaatacgc caattcttca gagagagacg
     2101 aatgaccgca gcgattccca cgaccaagcc cgctttccag tgggacgatc cgctgttgct
     2161 tgaggagcag ctgacggagg aagagcgcat ggtgcgcgat accgcgcgcg cctactgcca
     2221 ggaaaaactg atgccgcgcg tgctggaggc aaaccggcac gagaagttcg accgcgagat
     2281 cctgctcgag atgggcaagc tcggtttcct cggctcgacc ctttccggct acggctgcgc
     2341 cggcgtaaac catgtgtgct acggcctggt ggcgcgtgag gtggagcgcg tggacagcgg
     2401 ctaccgttcg gcgttgtcgg ttcaatcgag cctcgtcatg catcccatcc atgaattcgg
     2461 aaccgaagcg caacgcaaga aataccttcc gaagctcgcc agcggcgaat ggatcggctg
     2521 tttcggcctg accgagcccg atcacggctc cgaccccggc ggcatggtga cgcgggcgaa
     2581 aaaggtttcc agcggcttcg tgctgcaggg cgccaagatg tggatcagca acgcgcctta
     2641 cgccgacgtg ttcctgatct gggccaagga cgaatccggc gagatccgcg gctacctcct
     2701 ggaaaagggc atgaaggggt tgtccacgtc caggatcgag ggcaagttca gcctgcgcgt
     2761 ctctcccacc ggcgaagtgg tcatggagca ggtgttcgtt ccggaggaaa accttctgcc
     2821 cggcgcagcg ggattgaaag ggccattttc ctgcctcaac aaagcgcgct acggcatcgc
     2881 gtggggcgcg ctcggcgcgg ccgagttctg ctggcacgcg gcgcgcaact acgtcatgga
     2941 gcgcaggcag tttggccgcc cgctcgctgc caaccagttg atccagttga agctcgcgaa
     3001 catgcaaacc gagatcaccc tgggcttgca gggggcgttg cggctcggcc gcctgatgga
     3061 tgaagggcgc gcgacactcg agatgatctc cctgctcaag cgcaacgcct gcggaaaagc
     3121 gctcgacatc gcgcgcatga gccgtgacat gcacgggggc aacggcatcg cggacgagtt
     3181 ccacgtcatc cgccacgtga tgaacctcga ggcggtgaac acctacgagg gcacgcacga
     3241 catccacgcc ctcatcctcg gccgagccca gaccggcatt ccggcgttca gctgatccgg
     3301 gacaattgct cgacccagca ccgcgcggtg atggactacg aattgccccg gcgcaggttg
     3361 atggagcgca ccaccctgcg cggcaccgca acgtcgtagc cggatcgcag gccgccgtta
     3421 cggctcgtgc ttgcgaaggt gtaccggttc ccgcatccgc gcccgtatac ggatgttcag
     3481 catctcgacc gcaaccgcga acgccatggc gaagtagatg tagcccttcg ggatgtgcag
     3541 gtcccagccc tcgccgatca gcgccacgcc gaccagtatg aggaacgaga gcgcgagcat
     3601 cttgatcgtg gggtgacggt ccacgaacgc gccgatcggg cccgccgcga acagcatcac
     3661 gccgaccgcg gcgatgatcg cgacgaccat gatcacaagc tggtccacca tgccgaccgc
     3721 cgtgatcacg gagtcgagcg aaaagacgat gtcgatgatg gcgatctgca cgacgaccga
     3781 tcggaacgtc gccgcggcgg ccacgggcct gctgtccggt tcctcgcctt cgagcgagtc
     3841 atggatctcg tgcacgctct tccacaacag gaacaggccg ccgccgatga ggatcaggtc
     3901 gcgtcccgag atctctttgc cgaacagcgc gaagagcggc gcggtcagcg tcatgatcca
     3961 agtcaggaaa agtagcaggg cgagccgcgt cagcatcgcg agcgcaaggc cgatggtccg
     4021 ggccttcggt cgctcgtgct ccggtagccg tccgcaaagt atggtgatga agatgatgtt
     4081 gtccacgccg agcacgatct ccagcacggt cagcgtcgcc agtgcaagcc aggcatgcgg
     4141 gtcgagtagc cattccatgg gtcacctttc tctcatctgc cgaaaacgct tccgccggcg
     4201 cgaaagtcga accgcggcgg atccattctg gtaaagtcta gactagacag tatgattgct
     4261 gtgctccaac gctagatata tgtaaacgcg tgccattctc ctcgtccgca gcgcctgcag
     4321 gcgggtcctc ggtttccgac ccgatgagag ttcccgctgc ccctggaaaa cgacacccga
     4381 tgcggcacgc gagcctggca tgcgggatgc tcctgatcgc cagcacggcg gttgcacagt
     4441 cgcagatcta tccctcacgg gccgtgcgca tcatcgtgcc attcgcgccg gcgggcggga
     4501 ccgatatcct cgcgcgcagc ctcgcccaga agatgggcga gagcaccggt cagcagttca
     4561 tcgtggacaa ccgcgctggc ggcaacagca tcatcgggac cgacatcgtc gccaaatcga
     4621 cgccggacgg ttacacgctc ctgttcacga ccaatatcta caccatcaat ccgtggctgc
     4681 accagaagat gccgtacaac gccgagacgg atttcgcgcc gatcacgctg gcgggctctg
     4741 cgcccacact gctcgcggtg catccctcgc ttccggcaag aaccctccgg gaactcgtgg
     4801 cgctggcgaa atcgaagccg gggcaattga cgatgtcggc ggcgggcagc ggcacgcctt
     4861 cccacctttc cggagaattt ctaaaacaga cggcggggat cgacgtgctg atcgtccagt
     4921 acaagggaac cggcgcctcg ttgagcgatc tcgtcggcgg ccaggtcgcg atgtcgttcg
     4981 gcagccttcc cggtctggct cccctcgtca aatccggcaa gctgcgcgcg cttgcggtga
     5041 gcggagtgaa acgatcgtcg tcgctgccgg aggtgccaac ggtgatggaa acctatcccg
     5101 agttcgaggt cctcatctgg tacgggctat ttgcgcccgc ccgggtccag cgcgacatcg
     5161 tcaatcgcct gcacgccgag gtcgtcaagg cgctcgccaa cgaagaggcc cggcaacgac
     5221 tggccgtcca agggttcgat ccgggcggca tgacgccaga gcagttcgcc gataccatcc
     5281 gacgcgacat gtcgcgctgg cggaaagtca tcgccaaggc atcgatacgc gtcgaataac
     5341 ccgttcccct gacagcgttt tttcatattt tccccagcgg cagctgacac cgacgggtag
     5401 tgcgctggtc taatgtgaac ggactggtcc tgatcctgaa gctggctgga gtacacgcct
     5461 cgcttggtca tcgcgctgca acaaccttca ggacttctgt gagaaagcct tttcaaaacg
     5521 gctacttagg cgtaatattt ggcgacattc ttgaaagacc aaatactgtc atcgcatagg
     5581 ccgccgaccc gttaaagtca caaagaacgt tttcaaggaa ctgaatgaaa cccaccgaca
     5641 tcggtgcgcc tgactacttt cacaaggtag tggattgtca gtgggcctgt ccggcccaca
     5701 cccccgtccc cgaatacatc cggttgatcg ccgcaggccg ctacagcgac gcctacatgg
     5761 tcaactggta ttccaacgtc ttccccggca tcctcgggcg cacctgcgac cgcccctgcg
     5821 agccggcgtg ccggcgcggg cgggtcgagg aacgcaacgc ggagaagccc gagccggtcg
     5881 cgatctgccg tttgaagcgt gtggctgccg acaacaagga cgatattcgc ggccggctgc
     5941 ccaaggtccc taagcagaag aacggcaagc gcatcgcctg catcggcgcc ggtcccgcct
     6001 ctctcaccgt cgcccgcgac ctgctcccgc tcggctattc ggtcaccgtg tttgaccagg
     6061 acccccgcac gggcggcatg atctggtcgc agattccgcg cttccggctt ccgctcgagg
     6121 tgatcgacga ggaggttggc tatatcctcg atctcggcgt cgagttccgc ggcggcgtca
     6181 ggatcgaaag catgcagcag ctgctggcgg aaggctacga cgcgatcttc gtcggctgcg
     6241 gcgcgccgcg cggccgcgac ctcgacatcc cggggcgcaa ggaagccgcg tccaacatcc
     6301 atatcggcat cgactggctc tcgtcggtgt cattcggtca cgtggacaag atcggcaagc
     6361 acgtgatcgt tctcggtggc ggcaataccg ccatggactg ctgccgcacc tcgcggcgac
     6421 tcggcggcga ggacgtgaaa gtgatcgtcc gctcggggtt cgaggaaatg aaggcctcgc
     6481 cttgggagaa agaggacgcc atgcacgagg gcattcccat cctcaactac ctcgtgccca
     6541 gggcgttcac gcatgagaac ggcaggctca ccggcgtcac cttcgagaag gtgcgcgccg
     6601 agtacgatgc cgggggacgg cgcaacctcg tgcacaccgg cgagcccgac cagcacttcg
     6661 aatgcgacga cgtgctggtc gcggtcggac aggagaacgc ctttccgtgg atcgaacgcg
     6721 acatcggcat cgaattcgac aagcacagca tgcccaaggt ggataacgtc acgatgcagt
     6781 ccacgctgcc caatgtctta ttcggcggcg acgcggcatt cgggcccaag aacatcatct
     6841 gggcggtggc ccacggccat gaagcggcga tctcgatcga caaactgtgc cagggtgagg
     6901 acgtcaaaga gcgtccggca ccgatggtca acctgacttc gcagaagatg ggcatccacg
     6961 agtggagcta cgacaacgac atctcgctcg cgggccgcta caaggtcccc tggcgcgaca
     7021 agaagatcac gttaagggac gtcaaggtcg aggtcgagat gggcttcgac cccaaaaccg
     7081 gcctcgcgga ggcgcagcgt tgcctcaatt gcgacgtgca gacggtgttc acgggcaagc
     7141 tctgcatcga gtgcgatgcc tgcgtggaca tctgcccgat ggactgcatc acgttcacgg
     7201 acaacggcga ggaatccgaa ctgcgcgacc ggctgaatgc cccggccaat aacccgactc
     7261 aggatctcta cgtctccgac gcgctcaaga ccgggcgcat catggtgaag gacgaggacg
     7321 tctgcctgca ctgcgggctg tgcgccgagc gctgcccgac cggcgcctgg gacatgcaga
     7381 agttcctgct cgacatgacc cacgccggcc cggcgtgccg cagcccgaag aggaaagccg
     7441 catgaagcgg atagaggcgg taaacgactt cgtcgtcaaa ttcgccaacg tcaacggctc
     7501 gggctcggcc tccgccaacg agct
//
LOCUS       DBNGWZ010000532         9367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1458937, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000532 DBNGWZ010000000
VERSION     DBNGWZ010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1458937"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..2107
                     /locus_tag="AC16CX_16510"
     CDS             164..2107
                     /locus_tag="AC16CX_16510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3014146.1"
                     /translation="MTDKQTAEYSIQQVSEMLSIPIQKLRRWDEQGVLVAGRTQGGHR
                     RYSRELIDRLTGAGFSASDKANKELATIRKSLAEKRRIIQLLLESENRYRDLVETSHD
                     LIWTTDAQGRFTYLNAAANAIFGLAPKDLIGRCFFDFEAGTSHVSNRRFLAALKRNGE
                     VKHYVTRLVTAKGQDRWIGINARVVLDDNDAMLGIRGTARDITEEHLATRRIEHLALH
                     DALTDLPNRHSLQSHIEAALQSEGVGALLFLDIDHFKYVNDHFGHRTGDQLIVGVGSV
                     LCDLARNVDGELFRLGGDEFAIHLPSALRKDAVEVAEKALDAVRRYRFQAADNKVISN
                     LAASAGIALYPFHGADVLALLSNVDIAMYQAKDLGRNRHVLFDQASESLRSTHRRIHW
                     AKKLRDALDEDRLALFHQPVVRLKDRKPVHYEILVRIRDEAGGHILPGNFIEIAESLG
                     LIQEIDMRVVEKLLAYLRASGHANRKLRYFVNLSRVSISDQHWIQRFLAMLRDSGVNP
                     NQLVFEITETAAMSEIDVTLTFIKRLKEMGCRFALDDFGAGFSSFYYLKRFDVDYLKI
                     DGGFIRDLATDEGSRIFVRALNDVARGLDKQVIAEWVETPEVLNLLLGMGTQFGQGYL
                     FQRPAPLERGVRPDLDRSAASAA"
     gene            complement(2191..2745)
                     /locus_tag="AC16CX_16515"
     CDS             complement(2191..2745)
                     /locus_tag="AC16CX_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3014147.1"
                     /translation="MADREPKDFTEHTVSSKTVYRGKLLHVMEDEVRLPDGNRTRREH
                     IRHPGAVAMVPFIDPGTVMLVRQYRYSLARHFYEIPAGKIDHGEEPLRSAQRELREEC
                     GCEAGEWRHLATIHPCIGYSDERIELYLARGLIHIGHALDDGEFIETVTVGLAEALRW
                     VKDGKITDVKTIIGLLWADKFRET"
     gene            complement(2843..4291)
                     /gene="nuoN"
                     /locus_tag="AC16CX_16520"
     CDS             complement(2843..4291)
                     /gene="nuoN"
                     /locus_tag="AC16CX_16520"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR3014148.1"
                     /translation="MSYITPLAPAYPELFLLAMVCVILIADLFVSDDNRVVTYALTQA
                     ALAGAFAITYATATAEPVYTFSGMFVDDLMSDVLKLLVYLGVMVMLVYSRAYIAARGL
                     FRGELFVLALFATLGMMVMISASHLLTLYLGLELLSLSLYSMVALQRDSAVATEAAMK
                     YFVLGALASGMLLYGMSMLYGATGTLGIAQLADIILSGGARDVVLVFALVFIVAGLGF
                     KLGAVPFHMWVPDVYHGAPTAVTLFIGSAPKLAAFAFIMRLLVQGLGAERLVGEWQQM
                     LIVMAVLSLAIGNVTAIAQTNLKRMLAYSTIGHMGFLLLGILSGDIIGYGAGMFYVVV
                     YFLMNIGSFGMILLLSRSGFEAENLEDFKGLNQRSPWYAFVMLLLMFSMAGVPPTVGF
                     YAKLSVLQAVINAGYVWLAVVAVLFSLIGAFYYLRLVKLMYFDAPSDAAPISPRGEVR
                     VLMSANGLAMLVFGILPEPLMWLCTYSIQVSL"
     gene            complement(4310..5797)
                     /locus_tag="AC16CX_16525"
     CDS             complement(4310..5797)
                     /locus_tag="AC16CX_16525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR3014149.1"
                     /translation="MLAGFPLLSLAIWIPILSGVAVLATGSDRNAAAARVIALVGAVL
                     GFAVTLPLYTRFDTLAGGFQFIEITPWIETFNVNYHLGIDGISLLLILLNSFTTMLVV
                     IAGWEVIKQRVAQYMAAFLFLSGVMNGVFSALDALLFYVFFEATLIPMFIIIGVWGGP
                     NRVYAAVKFFLYTLAGSLLTLVALIYLYNVSEGSFSLLDYYQLPLPLVPQILIFIAFM
                     LSFAVKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFIRLSLPIVPDASHYL
                     AGFVIALSLIAVVYIGLVALVQADMKKLIAYSSISHMGFVTLGIFIFNTHGLEGAVLQ
                     MISHGFISGALFLCVGVLYDRVHSRLIADYGGVVNTMPVFAALFMLFAMANSGLPGTS
                     GFVGEFMVIIGAVKTSFWYAAAAATTLVFGAAYTLWMYKRVIFGAVANHHVASLSDLD
                     AREFLVLGLLAAAVVVMGVWPNPFAEVLHTPVNELLNHVAQTKLR"
     gene            complement(5805..7805)
                     /gene="nuoL"
                     /locus_tag="AC16CX_16530"
     CDS             complement(5805..7805)
                     /gene="nuoL"
                     /locus_tag="AC16CX_16530"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3014150.1"
                     /translation="MTRMQLLYLMVPIAPLIGAVVAGLFGWAIGRRGAHWVTILLMVV
                     STVASAVVFLDVLEDNTYNGAVYTWLASGNVRFEIGFLIDRLSATMMLVVSFVSLMVH
                     IYTIGYMADDPGYQRFFSYISLFTFFMLMLVSSNNFLQLFFGWEGVGLMSYLLIGFWY
                     VRPTATYAQLKAFLVNRVGDFGFLLGIALILMYFGTLDYAAVFATARTLAGNTLEILP
                     GEAWSLMTVICILLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMV
                     ARVSPLYELSETALSVVMVIGAITALFMALIAIVQNDIKRVVAYSTLSQLGYMTVALG
                     ASAYAAGIFHLVTHAFFKALLFLGAGSVIIALHHQQDMRHMGGLRKYMPVTYLTTLIG
                     AISSAGIPGFAGFFSKDSIIEAAHLSKLPGSDFAYLAVLVTVFVTALYTFRLVFMTFH
                     GPERFRGHEDHHGEHGAKAQGDQEQGHAPRESPAVVTVPLILLAIPSVCAGWFIGPVL
                     FGGYFGPAIAVAPAHDVLAQMGRDFQGVAAMMIHGLTTPALWLALAGVATAWYLYLRR
                     PDLPDVIKQKAGLVYTVLDRKYGFDEAYQFLFGDGAVRFGTGLWKGGDVAVIDGVFVD
                     GSARAVGWVASVIRLLQSGFIYHYAFAMIIGILVLLTMVFIA"
     gene            complement(7920..8225)
                     /gene="nuoK"
                     /locus_tag="AC16CX_16535"
     CDS             complement(7920..8225)
                     /gene="nuoK"
                     /locus_tag="AC16CX_16535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3014151.1"
                     /translation="MVSLSHYLVLGAILFAIAVIGIFLNRKNMIVLLMAIELMLLAVN
                     MNFVAFSHFLDDPSGQVFVFFILTVAAAESAIGLAILVVLFRNLNTINVEDLDSLKG"
     gene            complement(8304..8909)
                     /locus_tag="AC16CX_16540"
     CDS             complement(8304..8909)
                     /locus_tag="AC16CX_16540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3014152.1"
                     /translation="MTFNEFVFWFFAAILVLSALGVITARNPVHAALLLVLAFCTSAA
                     IWMQLQAEFLAIALVLVYVGAVMVLFLFVVMMLDINMARLREGFWNYLPLGALVALLL
                     VAEMWVTLEYLSGFTGMPAPPALPAGYSNTKELGRLVYTEYVYPFEIAAVILLVAIVS
                     AIALTLRRRKQSKYINPAQQIGVRREKRVRIVSMPSEKREE"
     gene            complement(9044..>9367)
                     /gene="nuoI"
                     /locus_tag="AC16CX_16545"
     CDS             complement(9044..>9367)
                     /gene="nuoI"
                     /locus_tag="AC16CX_16545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR3014153.1"
                     /translation="YPNGEERCIACKLCEAVCPALAITIESEQRADGTRRTTRYDIDL
                     TKCIFCGFCEESCPVDSIVETRILEYHGEKRGDLIYTKPMLLAIGDLYEEQIAKDRED
                     DAAYR"
BASE COUNT         1950 a         3014 c         2943 g         1460 t
ORIGIN      
        1 gccgcaccag atccactagg agtctgtcga atcagggtcc ggcaaactcc tcggccattg
       61 gccatcgcat gacggccctg atagactaat gcggcacaca atgcacaaga gccagcggcc
      121 tgcggccgcg gccaggcgcc cgacgcctga caggggagcg acggtgacgg acaagcagac
      181 ggcggaatac agcattcagc aagtgtctga aatgctcagt atcccgatcc agaagctgcg
      241 gcgctgggac gagcagggcg tgctggtggc ggggcgcacc caaggcgggc atcgccgcta
      301 ctccagggag ctgattgacc ggctgaccgg tgccggtttc tccgcaagcg acaaggccaa
      361 caaggagctg gcgacgatac gcaagtcgct cgccgaaaaa cgccgcatca ttcaactcct
      421 gctggagagc gagaaccgct atcgcgacct ggtcgagacc tcccacgatc tcatctggac
      481 caccgacgcg caggggcggt tcacctatct caacgccgcc gccaatgcca ttttcgggct
      541 cgcgccgaag gatctcatcg gccgctgctt cttcgatttc gaagccggca cttcccacgt
      601 ctcgaaccgg cggttcctgg cggcgctcaa gcgcaacggc gaggtcaagc attacgtgac
      661 gcgactggtc accgccaagg gacaggaccg ctggatcggc atcaacgcgc gcgtggtgct
      721 cgacgacaac gacgccatgc tcggcatccg cggcaccgcg cgcgacatca ccgaggagca
      781 cctggcgacg cggcgtatcg agcacctcgc gctgcacgat gcgctcacgg atctccccaa
      841 ccggcacagc ctgcaaagcc acatcgaggc ggccctgcag tcggagggcg tcggcgccct
      901 gctgttcctc gacatcgacc atttcaagta cgtcaacgac cacttcggtc accgcaccgg
      961 cgatcagctg atcgtggggg taggcagcgt gctgtgtgac ctggcgcgga acgtcgacgg
     1021 cgagctgttc cgcctcggcg gcgacgaatt cgcgatccac ctgccctcgg cgctgcgcaa
     1081 ggatgcggtc gaggtcgccg aaaaggcgct cgacgccgtt cgccgctacc gcttccaggc
     1141 ggccgacaac aaggtcatct ccaacctggc ggcctcggcg ggcatcgccc tctacccgtt
     1201 ccacggggcc gacgtgctgg cgctgttgtc gaacgtggac atcgcgatgt accaggccaa
     1261 ggacctcggc cgcaaccgcc acgtgctgtt cgatcaggct tccgagagcc tgcgcagcac
     1321 ccaccggcgc atccactggg cgaagaagtt gcgtgacgcg ctcgacgagg accgcctggc
     1381 gctgttccat cagccggtgg tgaggctgaa ggaccgcaag ccggtgcact acgagatact
     1441 ggtgcgcatc cgtgacgaag ccggcggcca catcctgccc ggcaatttca tcgagatcgc
     1501 ggaatcgctc ggcctgatcc aggaaatcga catgcgagtg gtggaaaagc tgctcgccta
     1561 tctgcgcgcc agcggccacg ccaaccggaa gctgcgttat ttcgtcaacc tgtcgcgggt
     1621 ctcgatctcg gaccagcact ggatacagcg cttcctcgcg atgctgcgcg acagcggcgt
     1681 caaccccaac cagctcgtgt tcgagatcac ggagacggcg gcgatgtcgg aaatcgacgt
     1741 cacgctcacc ttcatcaagc gcttgaagga gatgggctgt cgtttcgcgc tcgatgattt
     1801 cggggcgggt ttcagctcgt tctactacct gaagcgcttc gacgtcgatt acctcaagat
     1861 tgacggcggt ttcatccgcg acctcgccac cgacgagggc agccggatct tcgtgcgggc
     1921 gttgaacgac gtcgcgcgcg gcctcgacaa gcaggtgatc gcggagtggg tggagacccc
     1981 ggaagtgttg aacctgctgc tcggcatggg cacccagttc ggccagggct atctattcca
     2041 gcggccggcc ccgctcgagc gcggcgtcag gccggacctc gaccgcagcg ccgccagcgc
     2101 cgcctgaccc gcaaggcgaa agtcgaaagg cggaaggaaa gccgccgaac gacctactta
     2161 ctttcgactt tcgccttccg actttcgact tcaggtctcg cggaacttgt cggcccacag
     2221 caggccgatg atggtcttca cgtcggtgat tttcccgtct ttcacccagc gcagggcctc
     2281 ggcgaggccc acggtcaccg tctcgatgaa ttcgccgtcg tccagcgcgt ggcctatatg
     2341 gatgagcccc cgcgccaggt agagctcgat cctctcgtcg gaatagccga tgcaggggtg
     2401 gatggttgcg aggtggcgcc actcccccgc ctcgcagccg cattcctcgc gcaactcccg
     2461 ctgcgcgctg cgcagcggct cctcgccgtg gtcgatcttg cccgccggta tctcgtagaa
     2521 atgccgggcg agcgaatagc ggtactggcg caccagcatc accgtccccg ggtcgatgaa
     2581 cggcaccatc gcgacggcgc ccggatgccg gatgtgctcg cgccgggtac ggttgccgtc
     2641 aggcaaccgc acttcatcct ccatgacgtg caagagcttg ccacgataga cggttttcga
     2701 gctgacggta tgctcggtga aatccttcgg ttctcggtcg gccatggtca atctcggcgg
     2761 gcagtaagcg gtaggtggca agcgacatat ggcagcagtc aaaagccggg gctcgtcgct
     2821 cccggcttgc cgcctgccgt ttctagaggg acacctggat cgagtaggtg cacagccaca
     2881 tcagcggctc gggcaggatg ccgaacacca gcatcgccag gccgttcgcg ctcatcagca
     2941 cgcgcacttc gccacgcggc gagatcggcg ccgcgtccga cggcgcgtcg aaatacataa
     3001 gcttgaccag acgcaggtaa taaaacgccc cgatcagcga gaacagcacc gccaccacgg
     3061 cgagccagac gtagccggcg ttgatgacgg cctgcagcac cgaaagcttc gcgtagaaac
     3121 ccaccgtcgg cggcaccccg gccatcgaga acatcagcag cagcatgacg aaggcgtacc
     3181 acgggctgcg ctggttgagc cccttgaagt cttccaggtt ctccgcctcg aagccgctgc
     3241 gggagagcag caggatcatg ccgaaggagc cgatgttcat caggaagtag accacgacgt
     3301 agaacatgcc ggccccgtag ccgatgatgt cccccgacaa tatcccgagc aggaggaagc
     3361 ccatgtgccc gatcgtggaa taggcgagca tgcgcttgag attggtctgg gcgatcgcgg
     3421 tgacgttgcc gatcgcgagc gaaagcaccg ccatcacgat cagcatctgc tgccactccc
     3481 cgaccagccg ctccgcgccg aggccctgca ccagcagccg catgatgaag gcgaaagcgg
     3541 cgagcttggg cgccgagccg atgaacagcg tcaccgccgt cggcgcgccg tggtagacgt
     3601 ccggcaccca catgtggaac ggcaccgcgc ccagcttgaa gcccagcccc gccacgatga
     3661 acaccagcgc gaacaccagc acgacgtccc gggcgccgcc ggagagaatg atgtccgcca
     3721 gttgcgcgat gccgagcgtg cccgtcgcac cgtaaagcat ggacatgccg tagagcagca
     3781 tgcccgaggc gagcgctccc agcacgaagt acttcatcgc cgcctcggtc gccaccgccg
     3841 agtcgcgctg cagcgccacc atcgaataca gcgagagcga gagcagctcc agccccaggt
     3901 agagcgtgag caggtggctc gccgagatca tgaccatcat gcccagcgtg gcgaacagtg
     3961 cgagcacgaa gagctcgccg cggaacaggc cgcgcgccgc aatgtaggcc cgcgaataga
     4021 ccagcatcac catcacccca agatagacca gcagcttcag cacgtcggac atgaggtcgt
     4081 cgacgaacat gccgctgaag gtgtagaccg gctccgcggt cgcggtcgcg taggtgatcg
     4141 cgaaggcgcc agcgagagcc gcttgggtga gcgcgtaggt caccacgcgg ttgtcgtcgc
     4201 tcacgaagag gtccgcgatc aggattacgc ataccatcgc gagcaggaac agctcgggat
     4261 aggctggcgc gagcggcgtt atataggaca tcactgactc gctttccctt tatcgcagct
     4321 tggtttgcgc gacgtggttc agcagctcgt tgaccggagt gtgcagcacc tcggcgaacg
     4381 ggttgggcca gacgcccatc accacgaccg cagccgcgag caggcccagc accaggaact
     4441 cgcgcgcgtc gagatccgac agggacgcga cgtgatgatt cgccaccgct ccgaaaatca
     4501 cgcgcttgta catccacagc gtataggccg cgccgaacac cagcgtggtg gctgccgccg
     4561 cggcgtacca gaaactcgtc ttgaccgcgc cgatgatcac catgaactcg ccgacgaagc
     4621 cgctggtgcc ggggagcccc gagttcgcca tcgcgaacag catgaacagc gcggcgaata
     4681 cgggcatggt attgacgacg ccgccgtaat cggcgatcag ccgcgagtgg acgcggtcgt
     4741 agagcacgcc gacgcacaga aacagcgcgc ccgagatgaa tccgtgcgag atcatctgga
     4801 ggaccgcgcc ttccagcccg tgggtgttga agatgaagat gccgagcgtg acgaacccca
     4861 tgtgggagat cgaggagtag gcgatcagct tcttcatgtc ggcctgcacc agcgccacca
     4921 gcccgatgta caccaccgcg atgagcgaca gcgcgatcac gaatccggcg agataatggc
     4981 tcgcgtcggg cacgatcggc agcgagagcc ggatgaagcc gtaggcgccg agcttcagca
     5041 tgatggcggc gagcaccacc gaacccccgg tcggggcctc gacgtgcgca tccggcagcc
     5101 aggtgtgcac cggccacatc ggcaccttga ccgcgaatga caacatgaac gcgatgaaaa
     5161 tcaggatctg gggcacgagc ggcagcggca actggtagta gtcgagcaac gagaagctgc
     5221 cttcggaaac gttgtagagg tagatcagcg cgaccagggt gagcagcgag cccgcgagcg
     5281 tgtagaggaa aaacttgacc gcggcgtaga cccggttcgg tccgccccac acgccgatga
     5341 tgatgaacat cgggatcagc gtcgcctcga aaaacacgta gaacagcagc gcatccagcg
     5401 ccgagaacac gccgttcatg acgcccgaca agaacaggaa cgccgccatg tactgggcga
     5461 cccgttgttt gatcacctcc cagccagcga tcacgaccag catcgtggtg aagctgttga
     5521 ggaggatcag cagcagagat atgccgtcga tgccgaggtg gtaattgacg ttgaaggtct
     5581 caatccacgg cgtgatctcg atgaactgaa agccgcccgc cagggtatcg aagcgcgtgt
     5641 agagcggcag cgtgacggcg aatcccagca ccgcgccgac cagcgcgatc acgcgcgcgg
     5701 cggcggcatt gcggtccgag cctgtggcga gcacggcgac gccggacagg atcgggatcc
     5761 agatggcgag cgagaggagg gggaaaccgg caagcatggc gggctcaggc gatgaagacc
     5821 atggtcagca ggacgaggat gccgatgatc atggcgaagg cgtagtggta gatgaacccg
     5881 gactgcagta agcggatcac ggacgcgacc cagccgaccg cgcgcgccga tccgtcgacg
     5941 aacaccccgt cgatcaccgc cacgtccccg cccttccaca agccggtgcc gaagcgcacc
     6001 gcgccgtcgc cgaagaggaa ttgatacgcc tcgtcgaacc cgtacttgcg gtcgagaacg
     6061 gtataaacca gccccgcctt ctgcttgatg acgtcgggaa gatcgggccg cctgagatag
     6121 aggtaccacg cggtcgccac ccccgcgagg gcaagccaca aggcgggcgt cgtcagtccg
     6181 tggatcatca tggcggcgac gccctggaaa tcccggccca tctgcgccag cacgtcatgc
     6241 gccggcgcga ccgcgatggc ggggccgaaa taaccaccga acagcaccgg gccgatgaac
     6301 cagccggcgc agaccgaggg aatcgccaac aggatgagcg ggacggtgac caccgccggc
     6361 gattcacgcg gcgcgtgccc ttgctcttga tcgccttgcg ccttggcacc gtgctcgccg
     6421 tgatgatctt cgtgaccacg gaagcgctcc ggcccgtgga acgtcatgaa caccaggcgg
     6481 aaggtgtaaa gcgccgtgac gaacacggtg acgagcacgg cgagatacgc aaaatccgag
     6541 cccggcagct tcgagagatg ggccgcctcg atgatcgagt ccttcgagaa gaagccggcg
     6601 aagcccggga tgcccgcgct cgagatcgcg ccgatcagcg tggtgagata cgtgaccggc
     6661 atgtacttgc gcagcccccc catgtgacgc atgtcctgct ggtggtgcag cgcgatgatg
     6721 accgaccccg ccccgaggaa cagcagtgcc ttgaagaacg cgtgggtaac gaggtggaag
     6781 atgccggctg cgtaggccga ggcgccgagc gccaccgtca tgtagccgag ctgcgacagc
     6841 gtggagtacg ccaccacgcg cttgatgtcg ttctgcacga tcgcgatgag cgccatgaac
     6901 agcgccgtga tcgccccgat caccatgacg accgagagcg cggtttccga aagctcgtag
     6961 agcggagaca cgcgcgcgac catgaaaatg ccggcggtga ccatggtggc ggcatggatc
     7021 agcgccgaga tcggggtcgg cccttccatc gagtcgggca gccatacgtg caggggaaac
     7081 tgcgcgctct tgcccatcgc gccgacgaac agcaggatgc agatcacggt catcagcgac
     7141 cacgcctccc ccggcaggat ctcgagggtg ttgccggcaa gcgtcctggc ggtggcgaac
     7201 accgcagcgt agtcgagcgt gccgaagtac atcaggatga gcgcgatgcc cagcaggaag
     7261 ccgaaatcgc ccacgcggtt caccaggaac gccttgagct gggcataggt cgccgtgggc
     7321 cgcacgtacc agaagccgat caacaggtag gacatcagcc ccaccccttc ccagccgaag
     7381 aacagctgca ggaagttgtt cgaagacacg agcatcagca tgaagaacgt gaacagcgag
     7441 atgtagctga agaaacgctg gtagccgggg tcatccgcca tgtagccgat ggtgtagatg
     7501 tgcaccatca gcgagacgaa ggacaccacc agcatcatgg tcgcggacag ccggtcgatg
     7561 agaaagccga tctcgaagcg cacgttgccc gatgcgagcc aggtgtagac cgcgccgttg
     7621 taggtgttgt cctcgagcac atccaggaac acgacggccg acgcgacggt cgagaccacc
     7681 atcagcagga tcgtgaccca gtgcgcgccg cgccggccga tcgcccagcc gaacaggccg
     7741 gccacgaccg ctccgatgag cggagcaatc ggaaccatga ggtagagcag ctgcattcgc
     7801 gtcatcggtc gccggcagtt ctgagtgttg agtgttgagt gttgagtttc atcgtccaac
     7861 gcctgatcgt tagcctgtcg ttaactcaac actaaaaact caaaactaaa cactgcgttt
     7921 catcctttca ggctgtcgag gtcctcgaca ttgatcgtat tgaggttgcg gaacagcacc
     7981 accagtatcg ccaggccgat cgccgattcc gcagccgcca ctgtcaggat gaagaacacg
     8041 aagacctggc cggaggggtc gtcgaggaaa tgcgagaacg ccacgaagtt catgttgacg
     8101 gcgagcagca tcagctcgat cgccatcagc agcacgatca tgttcttgcg gttcaagaag
     8161 atgccgatca ccgcgatcgc gaacaggatc gcgcccagca ccaggtagtg cgacagcgaa
     8221 accaaggctt cccctttagt gaggtgtgag gagtaaggtg tgaggagaaa aaccgccgcg
     8281 cctctcacct cactcctaac ccctcactcc tcacgtttct ccgacggcat cgagacgatc
     8341 cgcacccgtt tctcgcgcct cacgccgatc tgctgcgccg gattgatgta cttgctctgc
     8401 ttgcggcggc gcagcgtaag cgcgatcgcc gagacgatcg cgaccagcag gatcaccgcc
     8461 gcgatctcga acggatagac gtattccgtg tacaccagcc gcccgagctc cttggtgttg
     8521 ctatagcccg ccggcaacgc cggcggtgcg ggcatgcccg taaaacccga aagatactcg
     8581 agcgtgaccc acatctccgc taccagcagc agcgcgacga gcgcgccgag cgggagatag
     8641 ttccagaagc cctcccgcag ccgcgccatg ttgatgtcga gcatcatcac cacgaacagg
     8701 aacagcacca tcacggcacc gacgtagacc agcaccagcg cgatggcgag gaattcggcc
     8761 tgcagctgca tccagatcgc cgcggacgtg cagaacgcca gcaccagcag cagcgccgcg
     8821 tgcaccggat tgcgcgcggt gatgacgccg agcgccgaga gcaccaggat cgcggcaaag
     8881 aaccagaaga cgaattcgtt gaaagtcatt gttcttcggg tgaacggtga acggtgaacg
     8941 gtgaacggat ccccgcttcg gaccacatca gctctgtctc tccccttgtc ctcaccgttt
     9001 actgttcact gttcactgct cactgctcac tattcatcgt tcactagcgg tatgcggcgt
     9061 cgtcctcgcg gtccttcgcg atctgctcct cgtagaggtc gccgatcgcg agcagcatcg
     9121 gcttggtgta gatgagatcg ccgcgcttct cgccgtggta ctccaggatg cgcgtctcca
     9181 cgatcgaatc caccgggcac gactcctcgc agaagccgca gaagatgcac ttggtgaggt
     9241 cgatgtcgta gcgcgtggtg cggcgcgtgc cgtcggcgcg ctgctcggat tcgatggtga
     9301 tggcgagcgc cgggcacacc gcctcgcaca gcttgcaggc gatgcagcgc tcctcgccgt
     9361 tcggata
//
LOCUS       DBNGWZ010000533        24855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1081091, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000533 DBNGWZ010000000
VERSION     DBNGWZ010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 24855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24855
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1081091"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="AC16CX_16550"
     CDS             <1..983
                     /locus_tag="AC16CX_16550"
                     /inference="COORDINATES: protein motif:HMM:NF015803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HIR3014154.1"
                     /translation="NVFDRDHVFTAQFITSPTKYQDVNVVGAGYKIPFYSLGDSIEFV
                     AGYSKVDSGTVQDLFQVSGQGAIYGVRYNHSLPKWRDLEHKLIYGLDYRAYQNLVTPT
                     GTQNNLVPDITVHPFSLTYSASLRTTGQDLSFYLNMAQNIPGGNDGVDEDFKQPGARF
                     QVGTAGYRLFRAGFNYVRVLKDDYQFRVNFSSQYTDDALVAPEQFGVGGADNVRGFNE
                     RYVSNDKGYRSSWEIYTPDVAKAWRLENGRLRFLAFYDTAAVYRNYRALTEKEAAHLD
                     SGGIGLRWNYKNYYSARLDIAHVFHDGSGFSEPDNRRNIKKLHFSMAWVW"
     gene            complement(964..1290)
                     /locus_tag="AC16CX_16555"
     CDS             complement(964..1290)
                     /locus_tag="AC16CX_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3014155.1"
                     /translation="MIAPLFPEVAVREDGRGRPVTDTRAVMNGVLWILRTGSPWADLP
                     GRFPPHQTCHRRFKQWVRADVLRQILEVLAEDLRSWGKLDLSGCFIDAAFLAAKRGLW
                     STKPMP"
     gene            1685..2395
                     /locus_tag="AC16CX_16560"
     CDS             1685..2395
                     /locus_tag="AC16CX_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3014156.1"
                     /translation="MPAQFALNPQLLKSVPLFSLFSDAQLVQLLSCVQHRSYPRNSFI
                     LRAGEETDALYIVLSGRVKVLIPDEEGHEVILSIMGPHEFFGEMGLLDEQVRSASVET
                     LEPCEMLRMSKAGFTNILKDNFELAMLIIRNLVRRLREADRKIESLALIDVYGRVARL
                     LIDMAQFVDGKWVVEHAPPKQEIARMIGASREMVSRVVKDLHRKGLIRAEKRRIYVLD
                     KQSMQRRASSRHGDRHHP"
     gene            2626..3348
                     /locus_tag="AC16CX_16565"
     CDS             2626..3348
                     /locus_tag="AC16CX_16565"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3014157.1"
                     /translation="MNIHSLVLRQAGFLLLSLFSAAVMAASAGTVTHLSGTLSVQRPD
                     GSVRILSQKSEVNPGDVLTTQRDSYAQINFSDGSSLTMRPNTQIKVEQYSFVQDRPQD
                     DNSFLRLLKGGLRTVTGLVGKRGNQDAYKIGTATATIGIRGSSGDTIDNSDGKCEGVT
                     PGCEKLPAGVYHTTYTGSYIMQNEAGSQIIGEGQFGFARDPKTPPVILPGDPGLNLSQ
                     LPFTLGIGGGRIQGPGQECVVR"
     gene            3524..5821
                     /locus_tag="AC16CX_16570"
     CDS             3524..5821
                     /locus_tag="AC16CX_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR3014158.1"
                     /translation="MLLSEKTSARAAKNPLPPKLAALVREFWWFALLGLALYLALILY
                     TYSKADPGWSHSTSVMVVHNAGGRVGAWLADVMLYVFGLSAYWWVVGCAVLVWWGFRR
                     IENVAQGDRRSYVVAAIGFIIVLVASSGVEAVRLHSLKAALPQAPGGILGAVVGGSLS
                     QALGFTGGTLILLVLFTAGLSLFSGVSWLTVLERLGGWSETVYLFLMQKLQEREDRRA
                     GEQAAIKREEVVEESKKKLEDHEPIRIEPPAVEIPKSARVEREKQVPLFENLPDSLLP
                     PLSLLDEPEKNLEVLSTETLEFTSRLIEKKLLDFGVEVKVVAAYPGPVITRYEIEPAV
                     GVKGSQIINLVKDLARALSVVSIRVIETIPGKSLMGLEIPNPTRQIVRLSEILSSQVY
                     ADITSPLPLALGKDIAGNPVVADLQRMPHLLIAGTTGSGKSVAINAMLLSLVYKSPPT
                     QVRLILIDPKMLELSIYEGIPHLLAPVVTDMRQAANALNWCTAEMDRRYKLMNALGVR
                     NMGGFNQKVREAAKARKPIPNPLSPTPESAEPLHEISLIVVVIDELADLMMVVGKKLE
                     ELVARLAQKARAAGIHLMLATQRPSVDVITGLIKANIPCRIAFQVSARVDSRTILDQM
                     GAEALLGQGDMLFLPPGSGFLQRVHGAFVADHEVHKVVDYLKKLAQPQYVDNVLEEPE
                     SEEGSEGALEGGDAESDPLYDQAVAIVLKTRRASISLVQRHLRIGYNRAARLIEQMER
                     AGLVSAMQSNGNRDVLAPASASEER"
     gene            5892..6398
                     /locus_tag="AC16CX_16575"
     CDS             5892..6398
                     /locus_tag="AC16CX_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3014159.1"
                     /translation="MKPLVGLLLALTAGVCLAAETYKWVDERGVTNYGEKPPANRQAR
                     PVDIRPGAVIETGGQFSQPIEAERRRSAEDPQRPQVAAAPVMAPMPPLLSARGMDFDI
                     YIRLQRGMTEGELLLRAGRPDYESLENLRHDIVKTFYYFPTVANPYTTVVTLRGGRIF
                     DIDRIRKF"
     gene            6398..7006
                     /gene="lolA"
                     /locus_tag="AC16CX_16580"
     CDS             6398..7006
                     /gene="lolA"
                     /locus_tag="AC16CX_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR3014160.1"
                     /translation="MSRKLFLLAVFLPGLAGASGSEVLKDLLEKTTTARARFAQMVLD
                     KNLRMLQQSTGTMQFSRPGKFRWEYDKPYQQLIVSDGSRVWLYDKDLNQVTVRRLDRA
                     LGSSPAALLAGSNEIEKSYTLSGAGNQEGLDWLEAMPRTQDTAFEKIKLGFGKSGLEA
                     MELHDQFGQITVIKFSIIERNAKLPPEAFRFTPPKGADVISE"
     gene            6999..8315
                     /locus_tag="AC16CX_16585"
     CDS             6999..8315
                     /locus_tag="AC16CX_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3014161.1"
                     /translation="MNDLFSKREPQAPLADRLRPHTLDEFVGQAHLLGPGKPLRLAFE
                     AGKPHSMILWGPPGTGKTTLARLMAQAFESEFIAISAVLSGVKDIRDAVNRAELVLQQ
                     SGRSTILFVDEVHRFNKAQQDAFLPHVERGLLTFIGATTENPSFEVNSALLSRAAVYV
                     LQPLTEEELGRLFDRARGTALNELEIAAAARELLIATADGDARRLYNMLEQIGIAAEE
                     AGHREVDEAFVRNALAQRLRRFDKEGEQFYDQISALHKSVRGSSPDAALYWLTRMLDG
                     GADPLYIGRRLVRMAVEDVGLADPRALRIALDACETYERLGSPEGELALAEAALYLAI
                     AAKSNAVYMAFGAAQELVRNDATRPVPEHLRNAPTRLMKELGYGRDYRYAHDEPGAYA
                     AGENYFPEGMKKPEFYHPTERGLEARIREHLERLRQLDAEAKRKKR"
     gene            complement(8349..9314)
                     /locus_tag="AC16CX_16590"
     CDS             complement(8349..9314)
                     /locus_tag="AC16CX_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014162.1"
                     /translation="MQRMMAFLATLVCAAFGGGALAQPFPSKAVRIIVAFPPGGGVDI
                     VARAMGPKLTEIWHQAIVVENRPGASGVIGTEAAARSAPDGHTLFLGTLGNLTANQYL
                     YPKMPVDPLKDLAPLTQVVAVHFVMVAHPSLPAKNVKELIALAKARPGQINYSSSGPG
                     GAPHLGVELFKSMAKVNLVHIPYKGSGPSFVDLLVGQVSLTCDSLLQSLPYIKGGRLW
                     ALAVLGGKRSPLLPDIATVGETVPGYELTNWFGMTVPAATPRDLINRLYDDISKVLQQ
                     ADFRERIANMGANVIGSPPDQFGAFMKSESAKWGRVIKEANIKAE"
     gene            9515..10876
                     /gene="serS"
                     /locus_tag="AC16CX_16595"
     CDS             9515..10876
                     /gene="serS"
                     /locus_tag="AC16CX_16595"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3014163.1"
                     /translation="MLDIQLLRSDLRAVAKRLADRGYTLDAAAFEALEANRKKVQTET
                     QELQARRNQLSRQIGQIKAKGEDASQLMAQVNAEAERLKALEDQLEHIQKDLETFLLN
                     VPNMPHASVPAGKSSEDNPEVRKVGTPRTFDFPVRDHVDVGTPLGLDFDVASKISGAR
                     FVLMKGQIARLHRALTQFMLDIHTRDHGYTEIYAPYLVNAETMRGTGQLPRFEEDLFK
                     VPRQSFDDDAVAEEMRGKSVSSVTPKMRDASLYLIPTAEVPVTNIVRSEILPLSDLPL
                     RFVCHTPCFRAEAGSYGKDTRGMIRQHQFDKVELVQIVHPQKSYELLEELTGHAEAIL
                     KQLELPYRVVVLCTADMGFAAAKTYDIEVWLPGQNAYREISSCSNFEAFQARRMQARF
                     RNEKNKTEFVHTLNGSGLAVGRTLIALLENYQRKDGGVDIPSVLQPYMGGLKILQTGD
                     GEG"
     gene            complement(10890..10979)
                     /locus_tag="AC16CX_16600"
     tRNA            complement(10890..10979)
                     /locus_tag="AC16CX_16600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10943..10945),aa:Ser,seq:cga)
     gene            11017..12069
                     /gene="mltB"
                     /locus_tag="AC16CX_16605"
     CDS             11017..12069
                     /gene="mltB"
                     /locus_tag="AC16CX_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HIR3014164.1"
                     /translation="MANKTSVSVKSRFVLPRETTAYIIGTSRIVLIATALVCAATATA
                     QRALPVEIEAFIGEMVQKHRFDAGSLRRLFAQVQPRPAVIRAISAPITARPWQEFRSL
                     YVNAARVNGGAAYWRRHAAVLARASREYGVPEEIIVATIGMETLYGRNTGDFRVLEAL
                     TMLAFNYPPRAELFRAELEEYLLLVREARFDAMRIRGSYAGAIGIPQFLPSSYRRYAV
                     DFDGDGRRDLMNTPADAIGSVANYYKAQGWRSGEPIVVPAETSGSGMDALLEGSIRPR
                     LKIAELKRLGVAPAVPVDDKTEAALFMVETENGPRYWLGFNNFYVITRYNRSVNYALA
                     VYELAREVRALARPEG"
     gene            complement(12021..13130)
                     /locus_tag="AC16CX_16610"
     CDS             complement(12021..13130)
                     /locus_tag="AC16CX_16610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014165.1"
                     /translation="MPLLAISRLYHSYGGHKVVEGLTFALNKGTIGCLLGASGCGKTT
                     VLRCIAGFEPLQAGEIWLNGRPVSRRDLLVPAEQRHVGMVFQDYALFPHLTVSENIAF
                     GLRDGDHAGRTHRVDEVLETVRLTALRDKYPDELSGGQQQRVALARALAPQPHLLLLD
                     EPFSNLDVELRAALSVEVREILKQQNTTAVLVTHDQHEAFNLADEIGIMRDGKIEQWD
                     TPYRLYHEPASRFVADFIGQGVFLPGTVLADGRIRLELGVFQGNVPASWHQGTVVDVL
                     LRPDDILHDDASPLQAQVLHKAFRGAEFLYTLQLPGGGRVMSLVPSHHNHAIGEKIGI
                     RLEIDHFVAFRRETVEISTDQPSGLASARTSRASS"
     gene            13238..14938
                     /locus_tag="AC16CX_16615"
     CDS             13238..14938
                     /locus_tag="AC16CX_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014166.1"
                     /translation="MPYGFQSLRLDRLTLAATVIGALCALPVASVFLNLFATGTPGVW
                     EHLAQTVLPEYAVNTLWLMLGVGTGVIAVGVATAWLTAMHDFPGRRVFEWALILPLSI
                     PAYVIAFVYTDLLQFVGPVQGWLREVTGWSKADYWFPDVRSLGGAIAMFILVLYPYVY
                     LLARTAFLERSTSLLEAGRTLGMGSAGVFFRIALPLARPAIVAGTALALMEAIADFGT
                     VSYFGVPTFTTGIYQAWFSMGDRIIAAQLSAALLGFVTLVLLLEHLSRGRRRFHETSQ
                     RARAPSRHRCTGLAAFAATLTCLVPLALGFLLPAGLLLGMTIAEGDAQIGARFVHLAL
                     NSVTLAAITAALLVMLALLMTYNARLNLGVFSQGLNRTVGLGYAVPGLVIAVGVLIPL
                     ARFDNALDAWMRTSFGISTGLLLTGSIAALVFAYIVRFFSVGLQTVEASLIKVRPSMD
                     DAARSLGCGPFEALLRIHVPIMARGLFAAALLVFVEVMKELPATLVVRPFNFDTLATQ
                     VYTLTQDERLAEASTAALAIVAVGLIPLIMISRNIAKTRGPARMGLAPFAAAVEAGTR
                     "
     gene            complement(14989..16029)
                     /locus_tag="AC16CX_16620"
     CDS             complement(14989..16029)
                     /locus_tag="AC16CX_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3014167.1"
                     /translation="MLNRLHIFPQLVTCLLLTVTYSLSGWAQDRVLNLYSSRHYQTDE
                     ALYNNFTKLTGIKINRIEGTEDALLERLKNEGPNSPADVLITVDVARLWRAQQMNLFV
                     PVKSAVLEKRIPAHLREPGGHWFGFSKRARVIVYNKQLVNPADVQDYEDLAHTKMKGK
                     VCTRSLGHVYNLSLMAALIDHLGEAKAQQWAQGVKANLARDPKGGDTDQIRSVAAGEC
                     QIAVSNTYYYVRLASSKKPEERAEAEKVGVIFPNQKSFGTHINISGGGVLRHAPNREN
                     AIRFLEYLAGEQAQSYFANGNNEWPAVPSTRLDNPALAALGVFKEDGLGVGALGQNQP
                     LAQKIFNLAGLK"
     gene            16210..17079
                     /locus_tag="AC16CX_16625"
     CDS             16210..17079
                     /locus_tag="AC16CX_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HIR3014168.1"
                     /translation="MRAVYTAGVLLCVTGTALSQTTVLEVIPLRYRTAQEVIPAIQPL
                     LARDGSVSGLQGQLIVRTTPANLEEIKRILASIDTVPRQLLITVRQDADADRGRSAIE
                     ASGSVGGEHGRVTIPGSREPRGGSVVIREGDDQVRVQVLEGHGTESDRNTQTVRVMEG
                     REAFVRVGQSVPVRERQVHRTVIGGKVVEQVVDNTQYRDIATGFYVLPRVSGDRVTLD
                     VSPQRESLSKRVPGGVNVQSLVTTVSGQLGEWMEIGSIDQDARGQQTVILGRTTTATR
                     DSRRVLIKVEEVR"
     gene            17212..19197
                     /gene="yoaA"
                     /locus_tag="AC16CX_16630"
     CDS             17212..19197
                     /gene="yoaA"
                     /locus_tag="AC16CX_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase YoaA"
                     /protein_id="HIR3014169.1"
                     /translation="MLDLETIFSARGPLARVVKDFRARPFQVEMAHAVAETIADRRVL
                     VAEAGTGTGKTFAYLVPTLLSGGKVIISTGTKTLQDQLFDRDIPTVRAALNVPVTVAV
                     LKGRANYVCHYHLGRALEDGRLPTLEEAQYLGEIARFARSTTSGDKADCTSVPESTGA
                     WLHATSTRENCLGSRCPHYDRCFVMEARRQALAADVVVVNHHLFFADLVLRDEGVAEL
                     LPACNTVILDEAHQLPETATLFFGESVSTSQLIGLARDARLESAAAAKDYAPLPEAAQ
                     ALDTAAHDLRLAFPEESGRMPARLLDGRKHFESALEAVLARLDELSVALKSQAERSEG
                     LMRCRERALTLAEGIERWRSGVDVARVKWIELFRNSLHLNSTPLSIAEIFSSQIEDHA
                     RAWVLTSATLSVNRDFEHYCGRMGLNDARTASWDSPYDFASQALLYIPERLPDPNTPE
                     HTEAVVEAALPIIEAAGGRAFMLFTSLRAMRNGYEMLEQAFDARGLDYPLLVQGEGSR
                     SEMLVRFRDLGNAVLVGSHSFWEGVDVRGEALSLVVIDKLPFAPPDDPVLAARIDRMS
                     REGRSAFLEYQLPEAVITLKQGAGRLIRDEGDRGVLMICDPRLLTRGYGKRILASLPP
                     MKLTRSAREAVAFFSRQTTSRSGAKPAKAPPTSTRRR"
     gene            complement(19229..20611)
                     /gene="rimO"
                     /locus_tag="AC16CX_16635"
     CDS             complement(19229..20611)
                     /gene="rimO"
                     /locus_tag="AC16CX_16635"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR3014170.1"
                     /translation="MTAAKAKAAKASPERAAQPRGSRAASLPRVGFVSLGCPKALVDS
                     ERILTQLRAEGYLISPTYDDADLVVVNTCGFIDSAIDESLGAIGEALAANGRVIVTGC
                     LGAKGDIVKRTHPRVLAVTGPHATGEVMQTVHRHLPQRHDPYTSLIPPQGIRLTPAHY
                     AYLKIAEGCNHRCTFCIIPSMRGPLVSRPIGEVMQEADSLVKAGVKELLVISQDTSAY
                     GVDVKYRTGFWGGRPVRTRLTELARALGGLGVWVRLHYVYPYPHVDEIVPLMADSRLL
                     PYLDVPFQHASRRILRLMKRPASAEDNLARIRRWREICPDITIRSTFIVGFPGETEAE
                     FEELLAFLEEARLDRVGCFAYSAVEGAAANALPGHVPEEVKEERRARFMNLQEQISAR
                     RLERKIWRTLTVLVDEVRADGAVARSSADAPEIDGVVHVAPDPDLKSGEFATVRVTRA
                     GTHDLWAELA"
     gene            complement(20571..21194)
                     /gene="phaR"
                     /locus_tag="AC16CX_16640"
     CDS             complement(20571..21194)
                     /gene="phaR"
                     /locus_tag="AC16CX_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR3014171.1"
                     /translation="MAQQPRIIKKYPNRRLYDTETSSYITLADVKKLVLEQVEFKVED
                     AKTHEDLTRSILLQIILEEEAAGAPMFSSDMLSQIIRFYGNAMQGMMGSYLEKNIQTF
                     IEMQKRLQDQSRQMYGDNPMLNADAWNEFIKMQGPAMQGLMGRYLEQSANAFMEMQQQ
                     LQQQTRNLFGNFPFPNFTGGSRQTSDAKSPVSPAHDSGKGEGGEGKS"
     gene            complement(21360..22103)
                     /gene="phbB"
                     /locus_tag="AC16CX_16645"
     CDS             complement(21360..22103)
                     /gene="phbB"
                     /locus_tag="AC16CX_16645"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR3014172.1"
                     /translation="MVQRVALVTGGMGGIGEAICVRLAKAGHKVVTTYSPSNTKAEAW
                     LKEMKGKGHDVHAMQVDITDFDACQKAAAKIQSEVGPVDILVNNAGITRDASFRKMGK
                     PDWDAVLRTNLDSVFNMTKPVCDGMTDRGWGRIINISSINGQKGAFGQTNYAAAKAGM
                     HGFTKSLAQEVARKGVTVNTISPGYIATKMVLAVPKDVLDGKIIPQIPVGRLGKPEEV
                     AELVAYLCLDEAGFMTGANLAINGGQHMY"
     gene            complement(22218..23957)
                     /locus_tag="AC16CX_16650"
     CDS             complement(22218..23957)
                     /locus_tag="AC16CX_16650"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3014173.1"
                     /translation="MNRPPDDLDAARGSIEALNRRLMEGITQPLDLNASFDIAEILRS
                     LTAGLAQDTERWLEIQNRYYRKRLELWAAYAQPWPGAPAQPVIEPDVSDRRFRAPEWR
                     KEPYFDYLAQSYLLTAHWLREVVDTTKLEPRTKKKIEFFARQFVDAMSPANFPWSNPE
                     ALKLAAETKGESLAIGLRNLAADIDRGMISMTDESAFEVGRNLAVTPGAVVLQNDFMQ
                     LIQYRPATATVFERPLIMVPPCINKYYILDLQPENSFVRYAVEQGHTVFMVSWRNMPP
                     EMGRATWDDYLEHGVMKALTVAAEVCGVGKVNTLGFCVGGTLLGAALAVLRARNDERV
                     ASLTLLATMLDFTDAGELSVFVDEAYVRKREQDFAQGGVLHGRELALTFASLRANDLI
                     WNYVVNNYLKGRTPEPFDLLYWNGDSTNLPGAMYTYYVRNTYLENNLRVPGKLAMCGV
                     PVDLGRVDMPAYLLATREDHIVPWRTAYASTRLLGGKIEFVLSASGHIAGVINPASKN
                     RRNYWLNPNLEEDPEHWLAAATPQPGSWWQHWANWLAGFGGAQVPARAGLGGGIRQEI
                     EPAPGRYVKEKCG"
     gene            complement(23979..24140)
                     /locus_tag="AC16CX_16655"
     CDS             complement(23979..24140)
                     /locus_tag="AC16CX_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014174.1"
                     /translation="MLIALGVATIWLVGDLVHTHASRRRADTSAAPDRRGIPATLEIP
                     SSARLELMV"
     gene            complement(24403..>24855)
                     /gene="pgeF"
                     /locus_tag="AC16CX_16660"
     CDS             complement(24403..>24855)
                     /gene="pgeF"
                     /locus_tag="AC16CX_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR3014175.1"
                     /translation="PETVCVALVADCIPVLVADRAGTTVAIAHAGWRGLARGVIENAV
                     GRMALTPEELIAYLGPGIGPRAFEVGADVRDAFLTRDAQAERAFTPHAPGKWLADLFL
                     LARQRLLRAGVGNIYGGGLCTYSDAKRFFSYRRDRATGRMAALIWRTN"
BASE COUNT         4696 a         7454 c         7902 g         4803 t
ORIGIN      
        1 cgaacgtctt cgatcgggat cacgtcttca ccgcgcaatt catcacctcg ccaacgaaat
       61 accaggacgt caatgttgtc ggcgctggat acaagatacc gttttattcg ctcggcgact
      121 cgatcgagtt cgtcgccggg tactcgaaag tggattcggg cacggtccag gacctgttcc
      181 aggtcagcgg ccagggcgcg atttacggag tgcgctataa ccactccctc cccaagtggc
      241 gtgatctcga gcacaaactc atttacggtc tcgactaccg cgcctatcag aacctggtca
      301 ctccgacggg gacgcagaac aatctcgttc cggacatcac cgtgcatccg ttttccctca
      361 cctacagcgc aagccttcga accaccgggc aggacctcag tttctacctg aacatggctc
      421 agaacatccc ggggggaaac gatggcgtgg acgaggattt caagcaaccg ggcgcccgat
      481 ttcaggtggg aacggcaggc taccggctgt tccgggccgg ctttaattac gtcagggtgt
      541 tgaaagacga ttaccagttc cgggtgaatt tcagctcgca atataccgat gatgctctgg
      601 tcgcgcccga gcagtttggc gtcggcggag ccgacaacgt caggggattc aacgagcgct
      661 acgtgtcgaa cgataagggt tatcgcagca gttgggagat ctatacgccg gacgttgcca
      721 aggcatggcg tctggaaaac ggccggttgc ggtttttagc cttttatgac accgctgcag
      781 tctatcgaaa ctacagggcg ctcactgaga aggaagcggc acatttggat agcggcggga
      841 tcggcttgcg gtggaattac aagaattatt attcggcacg tctggatatc gcccatgtgt
      901 tccatgacgg cagtggattc agcgagccgg ataacagacg aaatatcaag aagcttcatt
      961 tctctatggc atgggtttgg tagaccagag cccccttttc gctgctaaga aggccgcgtc
     1021 gatgaagcac ccggagaggt cgagcttccc ccaacttcgc aggtcctccg caagcacttc
     1081 caggatctgc cgcagcacgt cggccctcac ccattgcttg aagcggcgat ggcaggtctg
     1141 gtgtggggga aagcgccccg gcaaatcggc ccacggggaa cccgtgcgca atatccacag
     1201 caccccgttc atgaccgccc gcgtatcggt gaccgggcgt ccgcgcccgt cttcccgcac
     1261 cgccacttcc ggaaacagcg gtgcaatcac tgtccattgc tctcggtgag ctcctcgcgt
     1321 cgcgctatcc atcaccctcc cggccatgag atggactaac tttagaaaaa aacgacgcaa
     1381 cttacacacg atcattcttg agatagcctt tagaaatggt gactaaggtc gctgttggag
     1441 agaaaacccg ggtcagaaag ccggacattt gcaaaatagc gtttaaccat aaccagtcac
     1501 atgggtagta gcccaaggct gcaaaaccga aggcccgacc tacggcacga ttcccatggc
     1561 tagaagggaa tcggccgtta gccgggtggc gtgacgccag acgcttgatc tgtcagacta
     1621 tggtcttgca tgcacggcac cgacaagcgg cgtgacgtaa aacaagaacc cggggtgggt
     1681 tgatatgcca gcacagttcg cgctgaatcc acagcttctc aagagcgtgc cactgttttc
     1741 cctgttttct gacgcgcagc ttgtccagtt gctgagttgc gtgcagcacc gcagctatcc
     1801 ccgtaattcc ttcattctgc gggcaggcga agaaaccgac gcgctctata tcgtcctctc
     1861 cggacgggtg aaggttctga ttcccgatga ggagggacac gaggtgattc tttccatcat
     1921 gggaccgcat gagttcttcg gggagatggg gttgctcgac gaacaggtgc gctcggcgag
     1981 cgtggaaacc ctggaaccat gcgagatgct gcgcatgtcc aaagccggtt tcacgaacat
     2041 cctcaaggac aatttcgaac ttgcgatgct gatcatccgc aacctggtca ggcgcctgcg
     2101 cgaagccgac cggaaaatcg aaagcctcgc cctgatcgac gtctacggac gcgtggcacg
     2161 attgttgatt gacatggcgc aattcgttga cggcaaatgg gtggtcgagc atgccccgcc
     2221 caagcaggaa atcgcgcgga tgatcggggc ttcacgcgag atggtcagcc gcgtggtcaa
     2281 ggacttgcac cgcaagggcc tcatccgcgc cgagaagcgc cgtatctacg tactcgacaa
     2341 gcaatccatg cagcggcggg cctccagccg ccacggggac cgccatcacc cctgattcag
     2401 ggtgacgtgc aacatcaccc gcactggccg aatccgtcag tgctaatccg ttttttccta
     2461 atcttttgat tttgagcaat tagttgctaa aaaggcaggc ccctgatgag cactttttca
     2521 ctgttcagaa cgccggcttg tggtataagt ttcattgttg ctacaacgag tttacggagc
     2581 ggcaatagtc cgggtgggtg gtgatgaccg gcgggggagt ggaccatgaa cattcacagt
     2641 ctagttctga gacaggcagg tttcttactg ttatcgctat tcagcgcggc agtcatggca
     2701 gctagcgcgg gcacggtgac gcacctcagc ggaacgcttt cggtgcagcg gcccgatggc
     2761 agcgtgcgca tcctgtcgca gaagtccgag gtgaatcccg gcgacgtact gacgacccag
     2821 cgcgacagct atgcgcagat caatttcagc gacggcagct cgctcacgat gaggcccaat
     2881 acccagatca aggtcgagca gtacagtttc gtacaggatc ggccgcagga tgacaattcg
     2941 ttcctgcgct tgctcaaggg cgggttgcgc acggtgaccg gcctcgtcgg caagcgtggc
     3001 aatcaagacg cctataaaat cggcaccgcc accgcgacga tcggaatccg aggctcttcg
     3061 ggcgacacga tcgacaattc cgacggcaag tgcgaaggcg tgactccggg gtgcgagaaa
     3121 ctcccggcgg gtgtctatca cacgacttat accggcagct acatcatgca gaacgaggcc
     3181 ggttctcaaa tcatcggtga aggccagttt ggcttcgcca gggatcccaa gacaccgcct
     3241 gtcatactac cgggcgatcc gggcctgaac ttgagccagc tgccattcac cctcgggata
     3301 ggtggcggcc gtatacaagg cccaggtcag gagtgcgtgg tgcgataggc agcacatcgg
     3361 caaaattaaa aaccccgcct tgcgcggggt ttgttttttt gtacgcggat ttgccgccca
     3421 gctcgcattg gaaccctccg gccgcgtctt gtagtacacc acccgcgagg ttcttaacat
     3481 gggtcctttc gatgtacaat caaaaagtta aacggaaaac ttgatgctgt taagcgagaa
     3541 aacatcagcg cgggcggcca agaatccact gccgcccaag ctggccgcgc tggtccggga
     3601 gttctggtgg ttcgcgctgc tcggattggc cctctatctc gccctgatcc tctataccta
     3661 cagcaaggcg gatcccggct ggtctcacag taccagcgtc atggttgtcc acaatgccgg
     3721 tggcagggtg ggagcgtggt tggcggacgt catgctctat gtatttggcc tgtctgcgta
     3781 ctggtgggtg gtgggctgcg cggtcctggt gtggtggggg ttccgtcgca tcgagaatgt
     3841 ggcgcaaggc gaccgtcgtt cctatgtcgt agcggcaatc gggttcatca tcgtgctggt
     3901 tgcgagcagc ggtgtcgagg ccgtacgcct gcacagcctg aaggcagcgc tgcctcaagc
     3961 gccgggaggg atattggggg cggtcgtcgg gggttccctg tcgcaggcgc ttggtttcac
     4021 cggcgggacg ctcatcctgc tcgtgttgtt cacggcgggc ctgagcctgt tctcaggcgt
     4081 gtcctggctg acggtgctcg agcgcctggg cggctggtcc gaaaccgtat acctgtttct
     4141 gatgcaaaaa ctacaggagc gggaagaccg gcgcgccggc gagcaggcgg cgatcaagcg
     4201 cgaggaggtt gtcgaggaaa gtaagaagaa actggaagac catgaaccga tccgcatcga
     4261 gccgcccgcg gtcgagatcc ccaagtcggc gcgcgtggag cgcgagaagc aggtgccgct
     4321 gttcgagaac ctgcccgatt cgcttctgcc cccgttgagc ctgctcgatg agccggagaa
     4381 gaatctcgaa gtattgagca ctgagaccct cgaattcacc tcgcgcctga tcgaaaagaa
     4441 gctgcttgac ttcggggtcg aggtcaaagt ggtcgcggcc tatcccggcc cggtcatcac
     4501 ccgctacgaa atcgagcctg cagtcggcgt gaagggcagc cagatcatca acctggtgaa
     4561 ggacctcgca cgggcgctgt cggtggtgag catacgcgtc atcgagacca taccgggaaa
     4621 gagcctgatg ggcctcgaga ttccgaatcc cacgcgccag atcgtgcgtc tgtcggagat
     4681 cctcagctcg caagtgtacg ccgacatcac ctcgccgctc cccctcgcat taggcaagga
     4741 catcgcgggc aatccggtgg tcgccgacct gcaacgtatg ccgcacctgc tgatcgccgg
     4801 cacaaccggc tcgggcaagt ccgtggcgat caatgccatg ctgctgtcgc tggtctacaa
     4861 atcgcctccc acccaggtga ggctgatcct gatcgatccg aagatgctcg agttgtccat
     4921 ttacgagggc attccgcacc ttttggcgcc ggtggtcacc gacatgcgtc aggccgcgaa
     4981 tgcgctcaac tggtgcaccg ccgaaatgga tcggcgctac aagctcatga acgcgctcgg
     5041 cgtccgcaac atgggggggt tcaaccagaa ggtgcgcgag gcggccaagg ccaggaaacc
     5101 gatacccaac ccgctctcac ccacacccga gagcgccgag cccttgcatg aaatatcgtt
     5161 gatcgtggtg gtcatagacg agcttgcgga cctcatgatg gtggtcggca aaaagctcga
     5221 ggagttggtc gcgcggctgg cgcagaaagc gcgcgcggcc ggcatccacc tcatgcttgc
     5281 gacacagcgt ccgtcggtcg acgtgatcac cggactcatc aaagccaata ttccctgtcg
     5341 tatcgcgttc caggtctccg ctcgcgtgga ctcgcgcacc attctcgacc agatgggagc
     5401 cgaggcgtta ctgggccagg gcgacatgct gttcctgccg cccggaagcg gcttcctgca
     5461 acgcgtgcac ggtgctttcg ttgccgacca tgaggtgcac aaagtagtgg actatctcaa
     5521 gaaactggcg cagccgcagt acgtggacaa cgtgctggaa gagccggaat cggaggaggg
     5581 gagcgagggt gccctggaag ggggagacgc agagtcggac cccctctatg accaagccgt
     5641 cgccatcgtg ctcaagaccc ggcgcgcctc gatttcactg gtgcagcgcc acctcagaat
     5701 cggctacaac cgcgccgcac ggctgatcga gcaaatggaa cgggccggcc tggtgtccgc
     5761 gatgcagtcc aacggcaacc gcgacgtgct ggcgccggcc tcggcatccg aggaacgcta
     5821 gtttccgtat agaatctcca gtgctggcag ctgtctcaag cagatataga cactgctggt
     5881 actcgatctc catgaaaccg ctggttggat tgctgcttgc tctcacggcc ggggtatgcc
     5941 tcgccgccga aacctacaag tgggtggacg agaggggcgt caccaattac ggcgaaaaac
     6001 ctccagccaa ccgtcaagcc agaccggtgg atatccgtcc cggcgccgtc atcgaaaccg
     6061 gcggccagtt cagccagcca atcgaggcag aaaggcggcg ttctgccgag gatccgcagc
     6121 ggccgcaagt tgccgcagcg ccggtgatgg cgccaatgcc gcctcttttg tcggcgcgcg
     6181 gcatggattt cgatatttac atccgcctgc aacgcggcat gaccgagggc gagctgctgt
     6241 tgcgcgccgg cagacccgac tacgaaagcc tggagaacct ccggcacgat atcgtgaaga
     6301 ctttttatta cttccccacc gtggccaatc cctacaccac cgtggtgacg ttgcgcggcg
     6361 ggcgcatttt cgacattgat cgcatcagga aattttgatg tccagaaagc tctttttgct
     6421 ggctgttttt ttgcccggct tggcaggcgc atcgggcagc gaggtcctca aggacctcct
     6481 cgaaaagacc actacggcac gggcgcgatt cgcgcaaatg gtgctcgaca agaacctcag
     6541 aatgctgcag cagtcgaccg gcaccatgca gttttccagg ccggggaaat tccggtggga
     6601 gtatgacaaa ccataccagc aactcatcgt cagtgacggc agccgggtct ggctttacga
     6661 caaggacctc aaccaggtaa cggtacggcg gctcgaccgg gcgctgggca gcagtccggc
     6721 ggccttgctt gcgggcagca acgagatcga gaagagctat acgttatccg gggcgggaaa
     6781 tcaggaagga ctcgattggc ttgaggcgat gccgcggacg caggacacgg cattcgagaa
     6841 aatcaagctg ggtttcggca agtcaggact ggaagcgatg gagttgcacg accagttcgg
     6901 tcagatcacg gtgatcaagt tttccattat cgagcgcaac gccaagctcc cgcctgaagc
     6961 gttccgcttc acgccgccca agggggcgga cgtcatcagt gaatgatctc ttctcgaaac
     7021 gcgagccgca ggccccactc gccgaccggc tccgaccgca cacgctcgac gagttcgtag
     7081 gccaggctca tctgctgggg ccgggcaagc cattacggct tgcgttcgag gcaggcaagc
     7141 cacattccat gatcctgtgg ggaccgccgg gcacgggcaa gaccacgctc gcacgtctga
     7201 tggcgcaagc cttcgaatcc gagttcatcg cgatttccgc ggttttgtcg ggggtgaagg
     7261 acatacgcga cgccgtgaac cgggccgagc ttgtcctcca gcagtccggc cgcagcacca
     7321 tcctgttcgt ggacgaggtg caccgcttca acaaggcgca gcaggatgct tttctgccgc
     7381 atgtggaacg gggattgctg accttcatcg gcgctaccac cgagaacccc tcttttgagg
     7441 tgaacagcgc gctgttgtcg agggccgctg tatacgtcct gcagccgctg actgaagaag
     7501 agctggggcg gctcttcgac cgcgcgcggg gtacggccct gaatgagctc gaaatcgctg
     7561 ccgccgcgag ggagttgctg attgcgacag ccgacggcga tgcgcgacgg ctctacaaca
     7621 tgctcgagca gatcggcatc gcggcggagg aggcgggaca tcgcgaggta gacgaggcat
     7681 tcgtccgcaa tgcactggca cagcggctgc gccgcttcga caaggaaggc gagcagttct
     7741 acgaccagat ctcggccttg cacaagtccg ttcgcggttc cagcccggac gcggcgcttt
     7801 actggcttac ccgcatgctg gacgggggtg ccgatcccct ctacatcggc aggcggctgg
     7861 tgcgcatggc ggtcgaggat gtcgggctcg cagacccgcg cgcgctgcgc attgcgctcg
     7921 acgcctgcga aacctacgag cggctgggct cgccggaagg ggagcttgcg ctggcggaag
     7981 cggcgttata cctggcgatc gccgcgaaaa gcaacgcagt ctacatggcg ttcggcgcgg
     8041 cgcaggagct ggtgcgtaac gacgcgacgc ggccggtccc ggaacacctg cgcaacgcgc
     8101 cgacccgtct catgaaagag ctcggttacg gacgcgacta ccgctatgcc cacgatgagc
     8161 cgggggcgta cgcggcaggg gaaaactatt tccccgaggg catgaagaaa cctgagttct
     8221 atcaccccac cgagcgcggt ttggaggccc ggatccgcga gcacctcgag cgcctgcgcc
     8281 agctcgatgc ggaggcaaag cgcaagaaac ggtgacttgt ggactgctgt taatgaccgg
     8341 ttgtctggtc attcggcctt gatattggct tccttgatga ccctgcccca tttggcgctc
     8401 tcggacttca tgaaggcgcc gaactgatcg ggagggctgc cgatgacatt cgctcccatg
     8461 ttcgcaatcc tttcgcggaa gtcggcctgc tgcagcacct tggagatgtc gtcgtaaagc
     8521 cggttgatca gatcgcgagg ggtcgcggca ggcacggtca tcccgaacca gttggtgagc
     8581 tcgtagcccg gtacggtttc gccgacggtg gctatgtcgg gcagaagcgg agagcgtttc
     8641 cctccgagga ccgccagcgc ccacagtctg cctcccttga tgtagggcaa cgactgcaac
     8701 aggctgtcgc aggtgaggga tacctgtccg accagcaggt cgacgaagct cgggccgctg
     8761 cccttatagg gtatatggac gaggtttacc ttggccatcg acttgaacag ttcaaccccg
     8821 agatgcggtg cgccgcccgg ccccgatgag gagtaattga tctggccggg gcgggccttg
     8881 gcgagcgcga tcagctcctt cacgttcttc gcgggcagcg aaggatgggc gaccatgacg
     8941 aagtgcaccg ccacgacttg ggtgagcggg gcaagatcct tcagcgggtc caccggcatc
     9001 ttcgggtata ggtactggtt ggccgtgaga ttgcccaggg tacccaggaa cagggtatgg
     9061 ccgtccggcg ccgagcgcgc cgccgcctcg gtgccgatga cccccgacgc cccggggcgg
     9121 ttctcgacga cgatggcctg gtgccagatc tcggtcagct taggtcccat ggcgcgcgcc
     9181 acgatgtcga cgccgccgcc cggaggaaag gcgacgatga tgcgtaccgc cttgctggga
     9241 aacggctgcg ccagcgcgcc gccgccaaac gccgcgcaca cgagagttgc gagaaacgcc
     9301 atcattcgct gcatgagaaa actcccagga tgtttgctcg ccgtaggctt aagcgctaag
     9361 tggaggcggt gcggataccg tgcactatgt tgatcttgcg gggctatgga atctagcggg
     9421 cgggtgacag gcatgtcaag acattggccg cattctgcat tctgcgataa tacgcggtcg
     9481 tcgcttcgtc ttgacttgtt cgatcggaat ttaagtgctc gatatccagt tgctgcgcag
     9541 tgacctccgg gcggtcgcga aacggctggc cgaccgcggc tacacgcttg atgccgccgc
     9601 attcgaggcg ctggaagcaa accgcaagaa ggttcagacc gagacgcaag agctccaggc
     9661 ccggcgcaac cagctttcca ggcagatcgg ccagatcaag gccaagggcg aggacgcttc
     9721 ccagttgatg gcgcaggtca acgcggaggc ggagcggctg aaggcgctgg aagaccaact
     9781 ggaacatatc cagaaggatc tagagacgtt cttactcaat gttcccaata tgcctcatgc
     9841 gagcgtgcct gcgggcaagt cctccgagga caacccggaa gtgcgcaagg tcggcacacc
     9901 gagaactttc gattttccgg tcagggacca tgtggacgtg ggcacgccac tcgggctgga
     9961 tttcgacgtg gcctcgaaga tcagcggcgc acgcttcgtg ctgatgaagg ggcagatcgc
    10021 gcggctgcac cgtgccctga cccagttcat gctggacata catacgcggg atcatggcta
    10081 tacggagatc tatgccccat atctcgtcaa tgcggaaacc atgcgcggca caggacagct
    10141 gcccagattc gaagaggatc tgttcaaagt tccgagacag tcattcgatg atgatgcggt
    10201 tgcggaagaa atgcggggga aatcggtttc ctcagtgacg ccgaaaatgc gtgacgccag
    10261 cctgtacttg attcctacag cagaggtgcc tgtcaccaac atcgtgcgta gtgaaattct
    10321 tccgctatct gacctgccac tgagattcgt atgccacacg ccttgtttcc gggcggaagc
    10381 ggggtcatac ggcaaggaca cgcgcgggat gatccggcaa caccaattcg acaaggtcga
    10441 gctggtgcag atcgtccacc cacagaagtc ctatgaactg ctggaagagc tgaccgggca
    10501 cgcagaggcg attctcaagc agcttgagct gccgtatcgc gtggtggttc tgtgcacggc
    10561 cgatatgggt ttcgctgcag ccaagaccta cgacatcgag gtctggctgc cgggacagaa
    10621 cgcttaccgt gagatctcgt cgtgcagcaa cttcgaagcg ttccaggccc gccgcatgca
    10681 ggcgcgcttt cgcaacgaga agaacaagac tgagttcgtg cacacgttga atggctccgg
    10741 tcttgcggtc ggccgaacgt tgatagccct gctcgagaac taccagcgca aggatggcgg
    10801 ggttgacatt cccagcgtcc ttcaacccta catgggaggg ctcaagatcc tccagactgg
    10861 cgacggtgaa ggataaatct tgagctgggt ggcggagagg gtgggattcg aacccacgat
    10921 acgcttgcgc gtataccggt tttcgagacc ggcccgttca accgctccgg cacctctcca
    10981 cgcgaaggcg cgattttagc cgaaaaggcg tgacggatgg ccaataaaac ctcggtgtcg
    11041 gtcaagtcac gctttgtcct tcctcgggaa acgaccgcct acatcatcgg cacatcccgc
    11101 atcgtactga tagctaccgc actggtttgc gctgcgacag ctaccgcaca gcgggcactt
    11161 ccagtcgaga tcgaagcctt tatcggggag atggtgcaga agcaccggtt cgatgccggc
    11221 tcgttgcgcc ggcttttcgc gcaagtgcag ccgcgcccgg ccgtcatccg tgcaatctca
    11281 gcgccgatta ctgcgcgccc atggcaagag ttccgcagcc tgtatgtaaa tgccgcgcgc
    11341 gtcaacggtg gcgccgccta ctggcggcgg cacgcggccg tgcttgcgag ggcgagccgt
    11401 gaatatggtg tgcccgagga gatcatcgtc gccaccatag gcatggagac actttatggt
    11461 cgcaatacag gagacttccg cgtgctggaa gcgctcacca tgctcgcgtt caactatcct
    11521 ccgcgggccg agctgttccg ggccgagctc gaggaatatc tgctgctcgt gcgcgaagcg
    11581 cggttcgatg caatgagaat tcggggctca tatgccggtg ccatcggcat accgcaattt
    11641 cttcccagca gctaccgccg ctacgcggtg gatttcgacg gcgacggcag gcgggatctc
    11701 atgaacacgc ccgcggatgc gattggcagc gtggccaact attacaaagc gcagggctgg
    11761 cgcagtggcg aacccatcgt cgtgccagcc gaaacaagtg gcagcggaat ggacgcgctg
    11821 ctggagggca gcatcagacc tcggctcaag attgccgaac tgaagcgtct cggcgtagca
    11881 ccggcagtcc cggtcgatga caaaactgag gcggcgctgt tcatggtaga gactgaaaac
    11941 gggccgcgct actggcttgg tttcaacaac ttctacgtga ttacgcgcta caaccgcagc
    12001 gtcaactatg cgcttgccgt ttacgagctt gcgcgggaag tgcgagcgct ggccaggccc
    12061 gagggttgat cggtgctgat ttcgacggtc tcgcgccgga acgcgacaaa atggtcgatc
    12121 tccaggcgga tgccgatctt ttcgccaatc gcgtgattat ggtggctcgg cacgagcgac
    12181 atcacgcggc caccgcccgg cagctgcaac gtgtagagaa actcggcgcc gcggaatgcc
    12241 ttgtgcagca cctgcgcctg cagcgggctc gcgtcatcgt gcaggatgtc atcgggccgc
    12301 agcagcacgt ctaccacggt tccctggtgc cagctggcgg gaacattgcc ctggaatacg
    12361 cccagctcta gccggatgcg gccgtcggcc aataccgtcc cggggagaaa cacgccttgg
    12421 ccgatgaaat cggcaacgaa gcggctcgcc ggctcgtggt agagacgata aggcgtatcc
    12481 cattgctcga tcttgccatc gcgcatgatg ccgatttcgt cggcgaggtt gaacgcctcg
    12541 tgctggtcgt gcgtgacgag gacggcggtg gtgttctgct gcttgaggat ctcccgcact
    12601 tcgacgctga gcgcggcgcg cagctcgaca tcgaggttgg aaaacggctc gtcgagcagg
    12661 agcaggtgcg gctgcggcgc cagtgcacgc gcgagcgcga cacgctgctg ctgtccgccc
    12721 gagagctcgt ccggatactt gtcgcgcagc gcggtcagcc gcacggtctc cagcacttcg
    12781 tcgacgcgat gcgtccgtcc ggcatggtct ccgtcgcgca gcccgaaggc gatattctcg
    12841 gagacggtca ggtgagggaa gagcgcgtag tcctggaata ccatgccgac atggcgctgt
    12901 tcggccggca ccagcaggtc acggcggctc accggacgac cgttgagcca gatctcaccc
    12961 gcctgcagcg gctcgaagcc ggcaatgcac cggagcacag tggttttgcc acagccgctg
    13021 gcgccgagca ggcaaccgat cgtgcctttg ttgagggcaa acgtgagacc ttcgacgacc
    13081 ttatgtccgc cgtaagaatg gtagagcctg ctgatagcga gaagcggcac ggtcagcggg
    13141 tcggctatca agcactcgtc agtgccatta ccggttactg agtgacgtat attcatagac
    13201 atacgtataa taataaatct catttgcatc ttttgccatg ccctacggat tccagagcct
    13261 gaggcttgac cgtctgacct tggccgccac ggtcatcggt gcgctttgcg cgctgccggt
    13321 ggccagcgtg ttcctcaacc tgttcgcgac gggcacaccc ggtgtgtggg agcacctcgc
    13381 gcaaacggtc ctgccggagt atgcagtcaa cacgctgtgg ctgatgttgg gggtcgggac
    13441 aggcgtcatt gcggtgggcg ttgccacggc ctggcttacg gcaatgcacg attttccggg
    13501 gcggcgcgta ttcgaatggg cgctgatttt gccgctctcg ataccggctt atgtgattgc
    13561 gttcgtctat accgatctgc tgcaattcgt cggcccggtg cagggctggc tgcgggaggt
    13621 gacgggttgg tcgaaagccg attactggtt tcccgacgtg cggtcgctgg ggggggcgat
    13681 cgcgatgttc atcctcgtcc tgtatcccta cgtttatctt cttgcccgta ccgcctttct
    13741 ggaacgatcc acgagcctgc tcgaagctgg acgcacgctc ggcatgggaa gcgcgggcgt
    13801 ttttttccga atagcacttc cgctcgcgcg cccggccatt gtcgccggca ctgcgcttgc
    13861 gctgatggaa gcgatcgccg atttcggcac cgtttcctat ttcggggtcc cgacgttcac
    13921 caccggcatc tatcaagctt ggttctcgat gggggacaga atcatcgcgg cgcagctgtc
    13981 tgccgcattg ctcggtttcg tgacgctggt gctgctgctc gagcatctga gccgcggccg
    14041 aaggcgtttc cacgaaactt cacagcgcgc gcgcgcgcct tcgcgtcacc ggtgcacggg
    14101 cctggcagct ttcgctgcta cgctgacctg cctggtgccg ctcgcgctcg gatttctgct
    14161 gcccgccggg ctgctgctcg gaatgacgat cgccgagggc gatgcccaga ttggcgcgcg
    14221 tttcgttcat ctggcgctga atagcgtgac gctcgccgca atcaccgcag cgctgctggt
    14281 gatgctggcg ctgctgatga cttacaacgc gcgcctcaac ttaggagtat tcagccaggg
    14341 cctgaaccgc acggtggggc tgggctacgc cgtcccgggc ctggtgatcg cggtgggcgt
    14401 gctgatcccg ctcgcgcgct tcgataatgc gctcgacgcc tggatgagga catcattcgg
    14461 gatctcgacg ggactgctgc tcacgggcag catcgcagcg ctcgtcttcg cgtacatcgt
    14521 gcggtttttt tccgtggggt tgcagaccgt ggaagcttcg ctcatcaaag tgcggccgag
    14581 catggatgat gctgcccgca gcctgggctg cggcccgttc gaggccctgc tgcggataca
    14641 cgtgccgatc atggcgcgcg ggctgttcgc cgcagcgctc ctggtattcg tggaagtcat
    14701 gaaggagctg cccgcgacac tggtcgtgcg accgttcaac ttcgacacgc tcgccacgca
    14761 ggtctatacc ctgacgcaag acgagcggct ggccgaggcc tcgacggcgg cgctggcaat
    14821 tgtcgccgtc ggcctgattc cgctgatcat gatctcgcgc aatatcgcga agacccgggg
    14881 tccggcacgg atggggcttg cgccctttgc cgccgcggtg gaagccggga cccggtagga
    14941 gtggcggtcc ctgccgaagc tgcggccgac gtgggaaacc cgaagcggtt atttcaaacc
    15001 cgccagattg aatatcttct gggcgagcgg ctggttctgc ccgagcgcgc cgacgcctag
    15061 tccgtcttcc ttgaagacgc ccagcgccgc gagcgccggg ttgtcgagac gggtgcttgg
    15121 cacggcgggc cattcattgt tgccattggc aaagtaggac tgggcttgtt cgcccgccag
    15181 gtattccagg aacctgatcg cgttctcgcg gttgggcgcg tggcgcagca cgccgccacc
    15241 cgagatattg atatgggttc cgaagctttt ctggttgggg aagatcacac ctaccttttc
    15301 cgcctccgcc cgctcttcgg gctttttcga gctcgcgagg cgcacgtagt aataggtatt
    15361 gctgaccgcg atttggcatt cgcccgccgc caccgagcga atctggtcgg tgtcgccgcc
    15421 cttgggatcg cgtgcgagat tggccttgac gccctgcgcc cattgctgcg ccttcgcttc
    15481 gccgaggtgg tcgatcagcg ccgccatcag ggacaggttg tagacatggc ccagcgagcg
    15541 tgtgcagacc ttgcccttca ttttcgtatg tgcgaggtcc tcgtagtcct gcacgtcggc
    15601 cgggttgacg agctgcttgt tgtagacgat tacgcgtgcg cgcttggaga atccgaacca
    15661 atggccgccg ggctcgcgca ggtgcgcggg gatgcgtttt tcgagcacgg ccgacttgac
    15721 cggcacgaag agattcatct gctgcgcgcg ccacagccgg gcgacgtcga ccgtgattag
    15781 cacatcggcc gggctgttcg gcccttcgtt cttgagtcgt tcgagcaagg cgtcctcggt
    15841 gccctcgata cggttgatct tgatcccggt gagcttggtg aagttgttgt agagcgcctc
    15901 gtcggtctgg taatgccggg aggaatacag attcaaaacc ctgtcctgcg cccacccact
    15961 gagggaatag gtgacagtca gcagcaagca ggtgacaagt tggggaaata tatgtaacct
    16021 gttgagcata aatgtaataa taaggaacgt aagttggtag ataggttaag acggtgacca
    16081 ttataacaag aatggttctc aaatacaata gtaatgagaa tcatttacag aatgcgtaaa
    16141 ctggctgtag atcactgcgt taatcaacgg atgtggtctt gggtaagaag gctgaccgca
    16201 gttatccggg tgcgcgcggt ttacaccgca ggggtgctgc tttgcgtcac cggcacggct
    16261 ctgtcccaga cgacggtgct cgaagtgatc ccattacgct atcgcacggc acaggaagtc
    16321 atacccgcga ttcaacccct gcttgcgcga gacggcagcg tgagtggttt gcagggccag
    16381 ctcatcgtgc gcaccacgcc tgccaacctg gaagaaatca agcggatcct ggcaagcatc
    16441 gataccgtcc cacggcaatt gctgatcacg gtgagacagg atgccgatgc cgaccgcggc
    16501 agaagcgcga tcgaggcatc cgggagcgtc ggaggcgagc atggtcgggt gaccatcccc
    16561 ggcagccgtg agccgcgtgg cggcagcgtg gtgatccgtg aaggtgatga ccaggtgcgg
    16621 gtgcaggtgc tcgaaggaca cggcacggaa agcgaccgca acacgcaaac ggtgcgggtc
    16681 atggaagggc gcgaggcttt cgttcgcgtt ggccaatcgg tgcctgtgcg cgagcggcaa
    16741 gtgcaccgca ccgtgatcgg agggaaggtg gtggagcagg tggtggacaa tacgcagtac
    16801 cgcgacatcg ccaccggatt ctacgtacta ccacgcgtct ccggcgaccg tgtcacactc
    16861 gacgtgagcc cgcaacgtga atcgctctcg aagcgggtcc ccggcggcgt gaacgtccag
    16921 agtctggtga cgaccgtgtc cggccagctc ggtgaatgga tggagattgg cagtatcgac
    16981 caggatgcaa gggggcagca gacagtgatc ctcggccgta ctaccaccgc cacgcgcgat
    17041 agccggcgcg tgctgatcaa ggtcgaggaa gtcaggtagt agtccggcga tattcgaacg
    17101 aattgccgtt gatcatcaac cggcagctgc tgtcatcctt gagattggcg gcacgacccc
    17161 accgtttacg ggtcaggtga cgggctcggt ggcgccggtt aacattcccg cgtgcttgat
    17221 ctcgagacga ttttctcggc acgggggcca ctggcgcggg tggtgaagga tttccgcgcg
    17281 cggccattcc aggtggagat ggcgcacgcg gtggcggaga ccatcgccga ccggcgcgtg
    17341 ctggttgccg aagccgggac cggcactggc aagaccttcg cctacttggt accgacgctg
    17401 ctatccggcg gtaaagttat catttccacc ggcaccaaga cgctgcagga tcagcttttt
    17461 gaccgcgaca ttcccaccgt acgcgcggca ctcaatgttc cggtaacggt tgcagtgctg
    17521 aagggccggg cgaattacgt ctgtcattat catctcggac gcgcgttgga agatgggcgc
    17581 ctgccgacgc tggaggaagc gcaatatctc ggggaaatag cacgctttgc ccgcagcacg
    17641 accagtggcg ataaggccga ctgtacatcg gttccggaga gcacgggcgc ttggctccat
    17701 gccacctcca cgcgcgagaa ctgcctcggc tctcgatgcc cgcattacga ccggtgcttc
    17761 gtcatggaag cgcgccggca ggcattggca gccgacgtgg tggtcgtcaa tcatcatctt
    17821 tttttcgccg acctggtgct gcgagacgaa ggtgtggccg agctgttgcc cgcctgcaac
    17881 acagtcatcc tggacgaggc acatcagctt cccgaaaccg cgaccctgtt tttcggcgag
    17941 agcgtttcga cctcacagct gatcgggctg gcgcgcgacg cgcggctcga aagcgccgcg
    18001 gcggcgaaag actacgcccc attgccggag gcggcgcagg cgctcgacac cgccgcacac
    18061 gacctgagac tcgcctttcc ggaggagagc ggccgcatgc cggcacgcct gctggacggc
    18121 cgcaaacatt tcgagtcggc cctggaagcg gtgctggcaa ggctcgacga gctatccgtg
    18181 gcactgaaaa gccaggcgga gaggagcgaa ggcctcatgc gctgccggga gcgggcgctc
    18241 acgctggcgg aggggatcga acgctggagg agcggcgtgg acgtggcccg cgtgaagtgg
    18301 atcgagctct tccggaattc cctgcacctc aattcaacac cgctttcgat cgccgagatt
    18361 ttcagcagcc agatcgagga tcacgcgcgg gcgtgggtcc tgacctccgc gacgctgtcg
    18421 gtcaaccgcg actttgaaca ttactgcgga cggatgggac tgaacgacgc gcgcactgcg
    18481 agttgggaca gcccttatga cttcgcgagc caggccctgc tctatatccc ggagcggctg
    18541 ccggatccca atacgccgga gcacacggaa gccgtcgtgg aggctgcgtt gccgatcatc
    18601 gaagcagcgg gcggcagggc gttcatgctg ttcacgagcc tgcgcgcgat gcgcaacggc
    18661 tacgaaatgc tcgagcaggc gttcgatgcc cgcggtctcg attatcccct gctcgtgcaa
    18721 ggcgagggct cacgctcgga aatgctggtg cgctttcgcg atctcggcaa cgccgtactg
    18781 gtgggcagcc actcgttctg ggagggggtg gacgtgcgcg gcgaggcgct ttcgctggtg
    18841 gtgatcgaca agctgccatt cgccccgcct gacgacccgg tgctcgccgc gcgcatagat
    18901 cggatgagcc gcgagggacg cagcgccttt ctcgagtatc aactgcccga ggcggtaatc
    18961 acactcaagc aaggagcggg ccggctgata cgggatgagg gcgatcgcgg ggtgctcatg
    19021 atctgcgatc cgcggcttct cacccgcggc tacggcaaac gcatcctggc gagcctcccg
    19081 cccatgaagc tcacccgcag cgcgcgggaa gcggtggcat tcttcagcag gcaaaccacg
    19141 agtcggtccg gcgccaagcc ggccaaagcg ccgccaacct caacgcgacg acgatgaggt
    19201 caatcaggct gaacgcgtgc tcaagccctc aggcgagctc tgcccacaag tcgtgggtac
    19261 cggcacgtgt tacgcgcact gtcgcgaatt caccggattt cagatcaggg tcgggtgcga
    19321 catggaccac gccgtcgatt tcgggggcgt cggcgctcga gcgcgcgacc gcgccgtccg
    19381 cacggacttc gtccaccagc acggtcaacg ttctccagat cttgcgctcg agccggcgcg
    19441 cgctgatttg ctcctgcaga ttcatgaagc gggcgcggcg ttcttccttg acttcctccg
    19501 gcacatggcc gggcagggcg ttggctgcag cgccttccac cgcagagtag gcgaagcagc
    19561 cgacgcggtc gagccgcgct tcctcgagga aggcgagcag ttcctcgaat tcggcctcgg
    19621 tctcgccggg gaagcccacg atgaaggtgc tgcggatggt gatgtcgggg cagatttccc
    19681 gccagcggcg gatgcgtgca aggttgtcct cggcgctggc gggccgcttc atgagcctca
    19741 atatcctgcg actcgcatgc tggaacggaa catcgaggta gggcagcagc ctgctatccg
    19801 ccatcaatgg gacgatttcg tccacgtgcg ggtagggata gacgtagtgc agccgtaccc
    19861 aaaccccgag cccgcccagc gcgcgggcaa gctcggttag acgcgtcctg accggccggc
    19921 cgccccagaa tccggtgcgg tacttcacgt ccacgccgta ggcgctggta tcctgcgaga
    19981 tgacgagaag ctccttcacg cccgccttta ccaggctatc ggcctcctgc atgacctctc
    20041 cgatcggccg gctcacgagc ggcccgcgca tcgaagggat gatgcagaag gtgcagcggt
    20101 gattgcagcc ttcggcgatc ttcaagtagg cgtaatgcgc gggcgtgagc ctgatgccct
    20161 gcggcgggat caggctcgtg taaggatcgt gccgctgcgg gagatgccgg tgtaccgtct
    20221 gcatcacttc cccggtggca tgcggcccgg tcaccgcaag cacgcgcgga tgcgtccttt
    20281 tgacgatgtc gcccttggcc ccgaggcagc cggtgacgat cactcttccg tttgcagcga
    20341 gcgcttcgcc aatcgccccg agcgattcgt cgatggcgct gtcgatgaaa ccgcaggtat
    20401 tgacgacgac aaggtcggcg tcatcataag tgggcgagat gagataccct tcggcgcgca
    20461 gctgcgtcag gatgcgttcg gaatccacca gcgccttcgg gcagccgagg gatacgaacc
    20521 cgaccctggg aagggaggcc gcacgcgagc cgcgcggctg cgccgcccgc tcaggacttg
    20581 ccttcgccgc cttcgccttt gccgctgtca tgcgccggcg acaccggtga tttggcgtcg
    20641 ctcgtctggc gggagccgcc cgtgaagttg gggaacggga agttgccgaa gagattgcgg
    20701 gtctgctgct ggagctgctg ctgcatttcc atgaaggcgt tcgcgctctg ctcgagatag
    20761 cggcccatca agccctgcat cgccggtccc tgcatcttga tgaactcgtt ccaggcatcc
    20821 gcgttgagca tcgggttgtc gccgtacatc tgccgcgact ggtcctggag gcgcttttgc
    20881 atctcgatga aggtctggat gttcttctcg aggtagctgc ccatcatgcc ctgcatggcg
    20941 ttgccgtaga agcggatgat ctgcgacagc atgtcgctcg agaacatcgg cgcacccgcg
    21001 gcttcctcct cgaggatgat ttgcagcagg atgctgcgcg tcagatcctc gtgggttttg
    21061 gcgtcctcca ccttgaattc gacctgctcc agcaccagct tcttgacgtc cgcaagcgtg
    21121 atgtaactgc ttgtttcagt atcgtaaagc cggcggttcg ggtacttttt gatgatccgg
    21181 ggctgttgcg ccatgggatg aacgcgctat tccgaggagc gctattattt cagaaccact
    21241 gctgcattgc aaactaagag gttgaaaaat atcgctaaat ctttgtgtgc tcgctccaat
    21301 cgcgcgttcg ttgcgttgca gaaaaccgac catccatgca cgcgctcggt gcggattcat
    21361 cagtacatgt gctggccccc gttgatcgcg agattggcgc cggtcatgaa gccggcttca
    21421 tcgaggcaaa gataggcgac gagctccgct acttcctcgg gcttgccgag ccgtccgacc
    21481 gggatctggg ggatgatctt tccgtcgagc acatccttgg gaaccgccag caccatcttg
    21541 gtggcgatat agcccgggga gatggtgttg actgtcacac ccttgcgcgc cacctcctgg
    21601 gccagcgact tggtgaagcc gtgcatgccg gctttcgcgg cggcgtagtt ggtctggccg
    21661 aaggcgcctt tctggccgtt gatcgaggaa atgttgatga tccggcccca gccccggtcc
    21721 gtcatgccgt cgcacaccgg cttggtcatg ttgaacacgc tgtcgaggtt ggtgcgcagg
    21781 accgcgtccc aatcgggctt gcccatcttc ctgaaagatg catcgcgcgt gatgcccgca
    21841 ttgttgacca ggatgtcgac ggggccgacc tcggactgga tcttagcagc cgccttctgg
    21901 caggcgtcga aatcggtgat gtccacctgc atcgcgtgga catcgtggcc cttgcccttc
    21961 atttccttga gccaggcttc ggctttggta ttgcttggcg agtaagtggt gacgactttg
    22021 tggcccgcct tggcgagccg cacgcagatc gcctcgccga ttccacccat gccgcctgta
    22081 acgagagcca ctcgttgtac catgttgtct cctccacgcg tatgaacttg aaaatgcgcg
    22141 agctgtgacc ggtgaggggt gagccgggaa cttcaacaaa tacggttttt acccctcacg
    22201 ggtcactgat caccgcgtca tccgcatttt tcctttacgt aacgcccggg ggcgggttcg
    22261 atctcctggc ggatgccgcc gccgagccca gcccgcgctg ggacctgcgc gccgccgaac
    22321 ccggcgagcc agttcgccca gtgttgccac cagctgcccg gttgcggcgt cgcggcggcg
    22381 agccagtgct cgggatcttc ctccagattc ggattcagcc agtaattgcg gcggttcttc
    22441 gacgccgggt tgatgacacc ggcgatgtgg ccgctcgctg acagcacaaa ttcgatttta
    22501 cctccaagca gccgggtact ggcgtaagcg gtacgccatg ggacgatgtg atcctcgcgg
    22561 gtcgcgagca gatacgccgg catgtccacc ctgccgaggt ccaccggcac gccgcacatg
    22621 gcgagcttcc cgggcacccg caggttgttc tcgaggtacg tgttgcggac ataataagtg
    22681 tacatggcgc cgggcaggtt ggtgctgtcc ccgttccagt agagcaggtc gaacggctcg
    22741 ggcgtgcggc ccttgaggta gttgttcacc acgtaattcc agatcaggtc gtttgcgcgc
    22801 aggctggcga aggtcagtgc gagctcgcgc ccgtgcagca cgccgccttg cgcgaagtcc
    22861 tgctcgcgct tgcgtacata ggcttcgtcc acgaacacgg agagctcgcc ggcgtcggtg
    22921 aaatcgagca tcgtggcaag cagggtcagg ctcgccacgc gctcgtcgtt ccgggcgcgc
    22981 aataccgcga gggctgcacc gagcagggtg ccgccgacgc aaaagccgag ggtgttcacc
    23041 ttcccgacgc cgcacacttc ggccgcgacg gtgagcgcct tcatgacgcc gtgctcgagg
    23101 taatcgtccc aggttgcgcg tcccatctcg ggcggcatgt tgcgccacga caccatgaac
    23161 acggtgtgcc cctgctcgac ggcgtagcgc acgaacgagt tttccggctg caggtcgagt
    23221 atgtagtact tgttgatgca gggcggcacc atgatcagtg gccgctcgaa cacggtagcg
    23281 gtcgcgggac ggtactggat cagttgcatg aagtcgttct gaaggaccac cgcgcccggc
    23341 gtgacggcga ggttgcggcc gacctcgaag gcggactcgt cggtcatcga tatcatgccc
    23401 ctgtcgatgt cggcggccag attgcgcagc ccgatggcca ggctctcgcc cttggtttcg
    23461 gcggccagct tcagcgcctc cggattgctc cacggaaagt tggccggcga catggcgtcg
    23521 acgaactggc gcgcgaagaa ctcgattttt ttcttggtcc gcggctcgag cttggtggtg
    23581 tccacgacct cgcgcagcca gtgggccgtc agcaggtagg actgcgctag gtaatcgaaa
    23641 tagggctctt tgcgccattc gggcgcccgg aaccggcggt cggagacgtc cggctcgatc
    23701 accggctgcg ccggcgcgcc gggccagggc tgcgcgtagg ccgcccacag ctcgaggcgc
    23761 ttgcgatagt aacggttctg gatctccagc cagcgttcgg tgtcctgggc caggccggcg
    23821 gtcagcgacc tgagtatctc ggcgatgtcg aagctggcgt tgaggtcaag cggctgggtg
    23881 atgccttcca tcaacctgcg gttgagtgcc tcaatggatc cgcgcgccgc gtccaggtcg
    23941 tcgggtgggc ggttcatggc gtacatactg aaccggtatt aaaccatcag ttcaaggcgc
    24001 gcggatgacg ggatttcgag cgtggcgggg attcccctcc ggtcaggcgc cgccgacgtg
    24061 tccgcgcgcc gccttgaggc gtgcgtatgc acgaggtcgc ccaccagcca gatggtggcg
    24121 acgccgagcg cgatcaacac gacctgctgc agcgtcgcat gcagttcggg ccgccggtgc
    24181 aggcgtcgct gtcactatcc atacgagcgt cgtcatcggc agggagcccc tgatcggtga
    24241 ccggaggacc gctggctggc catccgtgtc gagcaaagcg cgcaattata cgtgataaag
    24301 cgccgacccg tggccgggaa aagcgtgatt cgtgaatcgt gacgggtggg tcagaatagg
    24361 gttctctcaa atcacgactc accactcgcg actcacgcct tatcaattcg tgcgccagat
    24421 cagcgccgcc atgcggcccg tcgcgcggtc gcggcggtag gagaagaagc gcttggcatc
    24481 ggaataggtg cacaacccgc cgccgtagat atttcctacg ccggcccgga gcaggcgctg
    24541 gcgcgcgagc aggaacaggt cggccagcca tttgccgggg gcatgcggcg tgaatgcgcg
    24601 ctccgcctgc gcatcgcgcg tcagaaacgc gtcgcgcacg tccgcaccga cctcgaatgc
    24661 acgcgggccg atgccgggac cgaggtaggc gatcaattct tccggggtca gcgccatgcg
    24721 cccgaccgcg ttctcgatga cccccctcgc aagcccgcgc cagccggcat gcgcgatggc
    24781 cacggtggtt cccgcgcggt cggcgaccag caccgggatg caatcggcaa cgagcgcgac
    24841 gcacaccgtt tccgg
//
LOCUS       DBNGWZ010000534         2195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1836254, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000534 DBNGWZ010000000
VERSION     DBNGWZ010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1836254"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /gene="rpsU"
                     /locus_tag="AC16CX_16665"
     CDS             <1..127
                     /gene="rpsU"
                     /locus_tag="AC16CX_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR3014176.1"
                     /translation="TELRAREYYEKPTTARKRKLAAAIKRHHKKLRAQLLPPRMY"
     gene            251..697
                     /locus_tag="AC16CX_16670"
     CDS             251..697
                     /locus_tag="AC16CX_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3014177.1"
                     /translation="MSLKSRINDDVKAAMRSGDARRRDALRLLLAALKQREVDERKEL
                     ADTDVVTIIDKLIKQRRESIVQFEKGGRQDLARNEQFEIEVFQAYMPQAMTDAEIEAA
                     VAEALAATGAKAPSDMGRVMGALKGKLAGRADMSKVSALVKAKLAG"
     gene            992..>2195
                     /gene="dnaG"
                     /locus_tag="AC16CX_16675"
     CDS             992..>2195
                     /gene="dnaG"
                     /locus_tag="AC16CX_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR3014178.1"
                     /translation="MIPQSFIQELLGRVDIVEVVDRHVKLKRAGANYVACCPFHSEKT
                     PSFTVSPTKQFYHCFGCGAHGNAISFLMEYSGLGYVDAVRELAESVGMKMPEFEPRAR
                     KEAGPDLYEIMQRACDHYREQLKVSPRAIDYLKSRGLSGKIAARFGIGYAPPGWQSLQ
                     GVFSGKDTVSYGDKVLKDAGLVIDAEGGRRYDRFRDRIMFPILDQRGAVIAFGGRVIE
                     PVLDDARDPGPKYLNSPETPLFEKGRELYGLPQARSAIRASGRVVVVEGYMDVVALAQ
                     HSIEFAVATLGTATSAAHVQRLLRQTDEVVFCFDGDAAGMKAAWHALEVSAPHIADHK
                     SVRFLFLPPEHDPDSFVRERGREAFERALAESQSLSEFFVDGLRRRVDGGGMEGKSRV
                     IHEAKPFLQ"
BASE COUNT          392 a          706 c          732 g          365 t
ORIGIN      
        1 gaccgaattg cgcgcccgcg agtactacga aaaacccacc accgcgcgca agcggaagct
       61 cgccgccgcg atcaagcgcc atcacaagaa gctgcgcgcc cagctgctcc cgccgcggat
      121 gtattgaatt gccgtaagcg gtaagcagta agcggaaaag ggtgaaggat cggctccttc
      181 gccttttttg ttttcgtatg atgttgaccg ctttacgctc actgctgacc gctcaccgct
      241 taccgctaac atgtccctca aatcccgcat caacgatgac gtcaaagccg ccatgcgcag
      301 cggcgatgct cgccgccgcg atgcgctgcg gctgctgctc gccgccctca agcaacgcga
      361 ggtggacgag cgcaaggagc ttgccgacac cgatgtcgtg accatcatcg acaagctgat
      421 caagcagcgg cgcgagtcga tcgtccagtt cgaaaaggga gggcggcagg acctcgcgcg
      481 gaacgagcag ttcgagatcg aggttttcca ggcctatatg ccgcaggcca tgacggacgc
      541 cgagatcgag gccgcggtgg cggaggctct ggcggcgacc ggcgcgaaag cgccgtccga
      601 catgggcagg gtcatggggg cgctgaaggg caaacttgcg ggacgggccg acatgtccaa
      661 ggtttccgcg ctggtgaaag ccaagttggc aggatagtcg ccgcgactcg ttagccgcag
      721 gcccgtttgc gggagcggcg acagcgcccc gcatgaaacg cagcgggccg gagtaacgcc
      781 ttgttttctt gaacccctgt cgattgccgg gcacggcggg gcggttacgg attccgacgg
      841 cacccgcccc gccgctattt ttcttgttct tcttcgttcg ggcggggcta tactccgcct
      901 tgatgcggac cgcagcaaat cagcgcgcac ggcgggacgg gcgcgcaatc cagtcaccgg
      961 tcaccagtca cgagtcacgc cccatcaggc aatgattccg cagtcgttca tccaggagct
     1021 cctcggccgc gttgacatcg tcgaggtggt ggaccggcac gtgaagctga agcgggcggg
     1081 ggcgaactac gtcgcgtgct gcccgttcca cagcgagaag acgccttcct tcaccgtgag
     1141 cccgaccaag cagttctatc actgcttcgg ctgcggcgcc cacggcaacg ccatctcctt
     1201 ccttatggaa tattccgggc tcggctacgt tgacgcggta agggagctcg ccgaatcggt
     1261 cggcatgaag atgcccgaat tcgagccgcg cgcgcgaaaa gaggccggac ccgacctgta
     1321 cgagatcatg caacgcgcct gcgaccacta tcgcgagcag ctgaaggtct cgccgcgcgc
     1381 gatcgactac ctcaagagcc ggggcctgag cggcaagatc gcggcgcgct tcggcatcgg
     1441 ctacgcgccc ccgggctggc agagcctgca gggcgtgttc tcgggcaagg acaccgtttc
     1501 ctacggcgac aaggtcctga aggacgcggg cctggtcatc gacgcggagg gcgggcggcg
     1561 ctacgaccgc ttccgcgacc gcatcatgtt ccccatcctc gaccagcgcg gcgccgtgat
     1621 cgccttcggc ggccgggtga tcgagccggt cttggacgac gcccgggacc ccgggccgaa
     1681 gtacctgaac tcgccggaga cgccgctctt cgagaaaggc cgggagctgt acgggctgcc
     1741 gcaggcgcgc tcggccattc gcgcgtccgg ccgcgtggtc gtcgtcgaag gctacatgga
     1801 tgtggtcgcg ctcgcgcagc acagcatcga gttcgcggtc gccacgctcg ggacggcgac
     1861 gtcggccgcg cacgtccagc ggctgctgcg ccagaccgac gaggtcgtgt tctgcttcga
     1921 cggggatgcg gccgggatga aggcggcttg gcacgccctt gaggtcagcg cgccgcacat
     1981 tgcggaccac aagagcgtgc gcttcttgtt cttgccgcct gagcacgatc cggatagctt
     2041 tgtgcgagag cgcgggcgcg aggcgttcga gcgcgcgctc gccgagtcgc agtccctgtc
     2101 cgagttcttt gtcgacggcc tcaggagacg cgtcgatgga ggcgggatgg aaggaaagtc
     2161 ccgcgtaatt cacgaggcaa agccgttcct ccagg
//
LOCUS       DBNGWZ010000535         3723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_74997, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000535 DBNGWZ010000000
VERSION     DBNGWZ010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_74997"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="AC16CX_16680"
     CDS             <1..318
                     /locus_tag="AC16CX_16680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3014179.1"
                     /translation="GESMPIYEYRCGECGYQKEYLQRMNDAPLTDCPECGKRAFNKLV
                     TAAGFQLKGTGWYATDFKNNGAKPSAKTDKTADSSKTAESSTKEPSKSDAPCGSGACP
                     SCS"
     gene            441..1109
                     /locus_tag="AC16CX_16685"
     CDS             441..1109
                     /locus_tag="AC16CX_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR3014180.1"
                     /translation="MSKKHFFRRYFITGLLIWVPLVITLWVLNLLVSTMDQSLLLLPS
                     EWRTEAWLGVHIPGLGVALTLLIVFATGVLAANIIGQRLVSFWEGVLARIPVVNSIYT
                     GVKQVSDTLFAPGGQAFRKALLVQWPSPGMWTIAFLTGTPGGDVSNHLKGDYVSIYVP
                     STPNPTGGYFVMVPREAVIELDMTVDEALKYIISMGVVAPNGNGRRSGVARPVVAQQK
                     AVNQ"
     gene            1205..2983
                     /gene="aspS"
                     /locus_tag="AC16CX_16690"
     CDS             1205..2983
                     /gene="aspS"
                     /locus_tag="AC16CX_16690"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004550866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR3014181.1"
                     /translation="MRSEYCGLVSRKHLGQTVTLCGWVHRRRDHGGVIFIDLRDREGL
                     VQVVCDPDTPETFKTADRLRGEFVIRVEGRVRERPAGTVNPDLASGEVEVLARDIAIL
                     NASLPPAFQLDEDHLSETVRLENRVLDLRRPAMQKNLRLRHRVATAVRQFLDRHGFID
                     VETPMLTRSTPEGARDYLVPSRVHPGEFYALPQSPQLFKQLLMIAGYDRYYQIVRCFR
                     DEDLRADRQPEFTQIDIETSFLDQGEITALMEELIRHLFKEAIGAELPNPFPRLTYDE
                     AIARYGTDKPDLRVPFTLTELTDVMKGVAFKVFAGPANTPGGRVAGLLVPEGGGLTRT
                     EIDAYTEFVGGHGARGLAWIKFNDAAKGRDGMQSPIVKNLSDAALKAIVERSGAKDGD
                     LMFFGADRAQVVNDALGALRARIGHERGFAQPGWRPLWVLDFPMFEWDEEEKRWVAMH
                     HPFTSPADGHEDRLESDPGAARAKAHDMVLNGWEIGGGSVRIHRSEVQQKVFRALKIG
                     PDEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVALMAGADSIRDVIAFPKTQRAQCLL
                     THAPSAVDERQLRELHIRLRHKDRES"
     gene            3115..3480
                     /locus_tag="AC16CX_16695"
     CDS             3115..3480
                     /locus_tag="AC16CX_16695"
                     /EC_number="4.6.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046589 - ribonuclease T1 activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanyl-specific ribonuclease"
                     /protein_id="HIR3014182.1"
                     /translation="MLRFWLALFALLLPLAGSAPARGPTPDALPEIAAAELPEQARQT
                     VALIRKGGPYPYERDGVVFGNFEKRLPVQVRGYYWEYTVRTPGAKGRGARRIVAGKGG
                     ELYYTDDHYRSFRRIRESP"
BASE COUNT          643 a         1188 c         1239 g          653 t
ORIGIN      
        1 ggagagagca tgccaattta cgaatatcgt tgcggggagt gcggctacca gaaggaatat
       61 ctgcagcgca tgaatgatgc gccgctcacg gattgcccgg agtgcggcaa gcgcgcgttc
      121 aacaagctcg tcaccgctgc cggcttccag ttgaagggta ccggctggta cgccactgac
      181 ttcaagaaca acggcgcgaa gccctcagcc aagacggaca agaccgcgga cagctcaaag
      241 accgcggaaa gctccaccaa ggaaccgtcc aagtccgacg ccccctgcgg ctccggcgcc
      301 tgcccgagct gcagctgacg ggccgagcca acctcgggac ccgcgctggc acggtccctg
      361 ccgagtttag cgctctgcac tgaactgcgt cgtttcctgc tatgcttctg cgctctgttg
      421 cgccggcgca gcggctcgtt atgtccaaaa agcacttctt cagacgctac ttcatcaccg
      481 ggctcctgat ctgggtgcca ttggtgatca cgctgtgggt actcaatctg ctggtgagca
      541 cgatggacca gtcgctgctg ctgctgccct ccgagtggcg caccgaggca tggctcggcg
      601 tgcacatccc ggggctgggc gtcgcgctga cgctgctgat cgtgttcgcc acgggcgttc
      661 ttgccgccaa catcatcggg cagcggctgg tctcgttctg ggagggcgtg ctcgcgcgca
      721 tcccggtggt gaactcaatc tataccggcg tgaagcaggt gagcgacacg ctgtttgcgc
      781 cgggggggca ggcctttcgc aaggcgctgc tggttcaatg gccgagcccc gggatgtgga
      841 ccattgcctt tctcaccggc acgcccggtg gtgacgtctc gaaccacttg aagggcgact
      901 acgtgagcat ctacgttccg agcacaccca atccgaccgg cggttacttc gtgatggtgc
      961 cgcgggaagc cgtgatcgag ctcgacatga cggtggacga agcgctcaag tacatcattt
     1021 cgatgggggt ggtcgccccc aatggcaacg gccgcaggag cggcgtcgca cggcccgtgg
     1081 tcgcgcagca gaaggcggtt aatcaataac gaagcgttca gcgctcggct cttagcgatc
     1141 agccggaggt gcgcctcgtt ttaagctgac tgctgacagc tgatagctat ttcctttttg
     1201 agcaatgcgt agcgaatact gcggcctcgt cagccgcaaa cacctcggcc aaaccgtgac
     1261 gttgtgcggc tgggtgcatc gccgccgcga ccatggcggc gtgatcttca tcgatctgcg
     1321 cgaccgcgag ggcctggtgc aggtggtttg cgacccggac acgccggaaa ccttcaagac
     1381 cgcggaccgg cttcgcggcg agttcgtgat ccgcgtggaa ggaagggtcc gggagcgtcc
     1441 agccggcacg gtcaatcccg atctcgcgag cggagaggtg gaggtgctgg cgcgggacat
     1501 cgcgatcctc aatgcctcgc ttccgccggc gttccagctc gacgaagacc acctgtcgga
     1561 aacggtgcgg ctcgagaacc gcgtgctgga tctccggcgc cccgccatgc agaagaacct
     1621 gcggttgcgt caccgggtgg cgacggcggt gcgtcagttc ctcgatcgcc acggcttcat
     1681 cgacgtcgag accccgatgc tcacgcgctc gaccccggag ggagcacgcg actatctggt
     1741 gccctcgcgc gtgcaccccg gcgaattcta cgcgctgccg caatcgccgc agctgttcaa
     1801 gcagctcctg atgatcgccg gctacgaccg ctactaccag atcgtcaggt gcttccgcga
     1861 cgaggacctg cgcgccgacc gccagcccga attcacgcag atcgacatcg agacctcctt
     1921 tctcgaccag ggcgagatca cggctctcat ggaggagctg atccgccacc tgttcaagga
     1981 agcgatcgga gcggagcttc cgaacccctt tccacgcctc acctacgatg aggcgattgc
     2041 ccgctacggc accgacaagc cggacctgcg cgttccgttc acgctgaccg agctcaccga
     2101 cgtgatgaag ggcgtcgcgt tcaaggtgtt cgccggcccg gccaacacgc cgggtggccg
     2161 cgtggcgggg ctgctcgtgc cggagggcgg cgggctcacc cgcaccgaga tcgacgccta
     2221 taccgagttc gtcggcggtc acggcgccag ggggctcgcc tggatcaagt tcaacgacgc
     2281 cgccaaaggg cgcgacggca tgcaatcgcc gatcgtgaag aacctgtccg acgcggcgct
     2341 caaggcgatc gtcgagcgct ccggcgccaa ggacggcgac ctcatgttct tcggagcgga
     2401 ccgcgcgcag gtggtgaacg acgcgctcgg cgcgctgcgc gcgagaatcg ggcacgagcg
     2461 cggcttcgcg cagcccgggt ggcggccttt gtgggtgctc gacttcccga tgttcgagtg
     2521 ggacgaggag gaaaagcgct gggtggcgat gcatcatccg ttcacctcgc ccgccgacgg
     2581 acacgaagac cggctcgaat ccgacccggg cgcagcgcgg gccaaggcgc acgacatggt
     2641 gctgaacggc tgggagatcg gcggcggctc ggtgcgcatc caccgctcgg aggtgcagca
     2701 gaaggtgttc cgcgcgctca agatcggtcc cgatgaggcg caggccaaat tcggctttct
     2761 gctggatgcg ttgcaatacg gtgcgccgcc gcacggcggg atcgcgttcg ggctcgaccg
     2821 catcgtggcg ctgatggcgg gcgcggactc gattcgcgac gtgatcgcgt ttcccaagac
     2881 gcaacgcgcg caatgcctgc tcacgcacgc gccgagcgcg gtggacgagc ggcagctgcg
     2941 cgagctgcac atccgcctgc ggcacaagga ccgtgagtcg tgattcgtcc cgttccaatg
     3001 tccacgtcca gaggctagcg cgtggatcat cgacctcttt ctcgagagaa atcattggaa
     3061 cgggattcgt gagtcgtgag tcgactccgt cccggtttgc ctgtgtcacg cgcgatgctt
     3121 cggttctggc tggcgctgtt tgcgctcttg ctcccgttgg ccggttcggc tccggcgcgt
     3181 ggcccgacgc ccgatgccct gccggaaatc gccgcggcgg agctgccgga gcaggcgcgc
     3241 cagacggtcg ctctgatacg caagggcggg ccgtacccct acgagcgcga cggggtcgtg
     3301 ttcggcaact tcgagaaaag attgccagtg caggtgcgcg gctattactg ggagtacacc
     3361 gtgaggacgc cgggcgcgaa aggccgcggc gcgcggcgta tcgtcgccgg caagggcggg
     3421 gagctctatt acaccgatga ccactatcgc agtttccggc gcatccgcga gagtccgtga
     3481 atcgtgggtc gtggatcgtg gatcgaacat tgtttattct cgccgggtcg tttgaggacg
     3541 agtaaggctt cgacccacaa cccacgaccc acaatccacg catttaacat gaagtacgac
     3601 aagatcctcc gcgccggacg ttcgggcgtg tacgccgctc cgcgtttgcc ggggccgctg
     3661 cgggcagcgg cgaagcgcgt gggcatcgag tggtacgatc tcgacctcgc cggcgtggcg
     3721 gat
//
LOCUS       DBNGWZ010000536        10444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1335282, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000536 DBNGWZ010000000
VERSION     DBNGWZ010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1335282"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="AC16CX_16700"
     CDS             <1..488
                     /locus_tag="AC16CX_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014183.1"
                     /translation="TDPTREKEWDAWYVEHLRIMLTVPGIASAQRFKTDSPGYSPSLA
                     MYTVASAEVFRDPYYLSVRGMGEWLPLVPKQHYHRNLFEGLDAAPDVAEDSVLIVADR
                     DRPGPGPHGVALIWLKAVGIDLSTPHRGIAIVKRAAADSLAAAGSIGVYRPVTPRAVQ
                     R"
     gene            533..1582
                     /locus_tag="AC16CX_16705"
     CDS             533..1582
                     /locus_tag="AC16CX_16705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF047299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3014184.1"
                     /translation="MRDQFALIAGATGLIGRRIAAHLHRLGWEVIGLCRRPVPGGAVR
                     LISVDLTDAGDCRSKLAELGNVTHVFYAARYDHPEGAAESVEINAAMLANLVEAVEPA
                     AAGLHHIHAVHGTKYYGHHLGPLAVPAREDDPRAPAPNFYYAQEDYLRERQRGKRWTY
                     STSRPHTYCDASWDEARNIGLLIAVYAAVLRELGLPLGYPGTARSFRVATQFTALPLL
                     ARALAWMATEPRCANQAYNIVNGDAPRWSELWPRFAAYFGMQTGPEQPVRLADAMADK
                     EGVWQAVIRKHGLRPTALQSLVRWSYGNYVFAPEWDIISDGSKARGHGFAETVDSAKM
                     FLEIFEFLRAEKIVP"
     gene            1858..2823
                     /locus_tag="AC16CX_16710"
     CDS             1858..2823
                     /locus_tag="AC16CX_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014185.1"
                     /translation="MNVITSRRVLLAALLAAGVAQAQGYPAKTIRWIVPWPPGGGADV
                     LSRMLSPHLSEALKQQIVIDNRGGAAGNIGAEMAAKSPPDGYTIAFAYSGTHAINPSI
                     YRKMPFKESDFAPIIWLSSVPQVLVVHPSLPVKTVKELVALAKKRPGELTYGSSGSGA
                     FNHLTGALFGQMTGTRLVHVPYKGGGPAAVALLSGEITMILGEPASLVGFIKTERVRA
                     LAVTGAKRTPGLPNLPTIAESGVAGYEATSWNGMLAPAGTPADIIKRLNAEFNRIIGT
                     PDMKKRMLDNGYEPVGGSPERFGELIRAEIAKWAPVVAAAGVRVD"
     gene            2843..3496
                     /locus_tag="AC16CX_16715"
     CDS             2843..3496
                     /locus_tag="AC16CX_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014186.1"
                     /translation="MASGRMGLLVAGFNYATVAGDEFNDWYDTEHIPERERIKGFVNC
                     ERWLGADDPTVSIATYDLESLAVLQSPGYRAIAGANLSPWSRRITGRCQRICRFEAEQ
                     ISPGDRAAPRDAGGLLLFAMNAAGEFEAEFNAWYDEEHIPRLSKVGGCLCARRFRTTR
                     AISNGNHRYLALYHLATPEVCSSPAWKAAAETPWTLKLRPHMNDRMRLVLRRYQRKA"
     gene            3519..4493
                     /locus_tag="AC16CX_16720"
     CDS             3519..4493
                     /locus_tag="AC16CX_16720"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014187.1"
                     /translation="MATIQTRSLACTALLALAPALAAVADAAEYPARPIRYVVAFAPG
                     GINDILARIVGQKLNESWGQPVIVDNRPGAGGNLAAELVSKSTPDGYTFMNISTAHTI
                     SQTLYSKLNYSLERDLAPVVVLGSSPLIMVVHPGIPAKNVPELVAWAKKNRLIYASGG
                     VGVISHLSMEMFKVAAGIDATHVPYKGVGPAVPDLISGQAHVMFNAVPELFPFAKGGR
                     LRIIATLTEKRHPFMPEVPTMIEQGYKEFVMGNWTGIVAPVKTSKNVVNRLAAEITRI
                     IRSPETSKRLVEMGVDPLGGTPEEFGKLIRSEVARFGKAVRDSGAKAD"
     gene            4614..5582
                     /locus_tag="AC16CX_16725"
     CDS             4614..5582
                     /locus_tag="AC16CX_16725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014188.1"
                     /translation="MFARFAVISSLALAAALSSPAGAQQFPTRPIRWIVPVPPGATTD
                     IISRLIGGKMSEAWGQQVVVDNRTGGAFVIGTDIVAKSNPDGHTIGIFLTPHAVNPFV
                     LKNLPYDTIRDFQPITLVAIVPGVMVTNPNVPVASLKDVIELARTKPGQLNYGSPGPM
                     TSGHLSMELLNLTAGIKILHVPYKGGAPAILDLLGGQIQFVILGPPTVMGHLKAGRLK
                     AIATTAAKRSPGLPNTPTFAESGLPGYDTYEWYGLFAPARIPRAALAKLNGEVNRIIA
                     LPDIRERMLGLGAIPSGNSTEEFAAFVKGEMNRWGKVAQQVGLRPD"
     gene            5588..7705
                     /locus_tag="AC16CX_16730"
     CDS             5588..7705
                     /locus_tag="AC16CX_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3014189.1"
                     /translation="MNDNQRPPGRRAGAVWVGIDTGGTFTDLVAVELESGRHHYHKVP
                     TTTEDPARGILDGIAELLDRNRLARADVAFLVLGTTLATNAVLEGKWAPTGLITTAGF
                     RDVLELARQRRPHYFNLDIPKPMPPATRDCRIEVGGRIAHDGSEVAPLAEDEVRRAVE
                     RLKRKKVEAVAVCFLHAYANPAHEERAKALVAGLWPGAYLCSSSEVLAEFREFERFAT
                     ATVNASLMPIMDRYLERFGRGVADLGIRPVPCVMQSNGGAVSPGAVRRLPVNTFFSGP
                     AGGVIGASGLGERLGLPNLITFDMGGTSTDVCLIRDGEPAQKSERLMGGFPVRTRALD
                     IHTIGAGGGSIAWRDAGGLLKVGPQSAGAHPGPAAYGRGGTQATVTDANVVLGRLNPR
                     RLLGGRMSMHPEKARAVMDELAGRLGVDTARAAAGVIEIVNVNMMGAVRVISVEQGED
                     PRDFTLVAFGGAGPLHAADIARNMGIVKVLVPPRPGLLSAIGLLHADVRGDFSLTRLL
                     RAASENLQALNWGFEELRRRGANWLEGEGEARTGFNWFADLRYSGQNFELIMELKSGG
                     LDEAALSRLVNSFHRRHKDSYGYDMRGQPVEIVNLRLMVTAKRRIPPHESIRMKRGDP
                     RQALLERRRVWFPETGFVAAPVYERDRLPADCRITGPAVIEQMDTTTVVPPKARLRSD
                     RLGYLHLELEPVEQKRGATWAAA"
     gene            7690..9366
                     /locus_tag="AC16CX_16735"
     CDS             7690..9366
                     /locus_tag="AC16CX_16735"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3014190.1"
                     /translation="MGCGVDPIRAEVVARFLLAAAEEMGATLMRTAFSPNIKERADCS
                     TAIFDAAGEVVALAQRVPIHLGSMVGAVDEIRRRYREQDIRPGDMFIANDPYTGGGTH
                     LPDINIIAPVFLGKRIVAFVANIAHHADVGGMVPGSEAAVCKSIFQEGIRIPPVRIQR
                     AGKLNRDVLELILLNSRTPGERVGDLQAQFAANTVGMRSVLQLFDRYGPKQTQAAVAA
                     YLDFTEKRFRAAIERLPKGRYEAEDFLDGDAEGEVCRIRLALTVGKGRLHFDFTGSGP
                     QLAAARNIPYRALLATVYTVAKSLLDPEVPANAGYYRTLTIKAPPGCVVGPVPPAAVG
                     CRSISASVLGDVIAQALSRAMPEKALAGCGPHQLYVLSGTDPRTGAYFVNYETLAGGY
                     GARAGRDGVDGVRVHASGSSNLPVEALEHAYPFRVERYALRDGSGGTGRYRGGAGVER
                     DYRVLADGIVVSLSSERQHVPAKGMMGGRDGATGAFVLNPGTAEERALPAAAADVRLP
                     HGSLFRIRTPGGGGYGDPRGRARGAVERDLREERTTPAQARELCGWGEKT"
     gene            9363..>10444
                     /locus_tag="AC16CX_16740"
     CDS             9363..>10444
                     /locus_tag="AC16CX_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3014191.1"
                     /translation="MSRPRETDAIGSARANYPALERWTYLDVAGRGVLSRAVRAALDA
                     HLDERMLNGADKQKFFELIENTRAGFARLIGAAPEEVAFTKNISEGLNMIATAFHWRR
                     GDNVILCPGLEHPNNVYPWLNMRRYGLEVRAVAPRAGHIPVDEIAARIDGGTRVVTVS
                     TVTFAPGFRTDVDALGNVCRERGVLLLVDAAQSVGVLHTDVGRSKIDALAVSTAKGLL
                     GLYGMGFLYCRSDWAERLAPAYLARFGVDLGAAHEATMGGDEYRLMPGARRFDLGNYN
                     FAAAAAVEASMRELAAVGTERIERHAVGLAHALARGCLDLGLPVAGGAPGPHLAHIVT
                     VGEMGASHYGSEDERINRLHDHLVANR"
BASE COUNT         1721 a         3489 c         3603 g         1631 t
ORIGIN      
        1 tgaccgaccc aacccgcgaa aaggaatggg acgcgtggta tgtcgagcac ctgcgcatca
       61 tgctgacggt gcccggcatc gcgagcgccc agcgcttcaa gaccgattcg cccggctatt
      121 cgccgtcgct tgcgatgtac accgtcgctt ccgccgaggt cttccgcgat ccgtactacc
      181 tgagcgtgcg cgggatgggc gaatggctgc cgctcgtccc gaagcagcat taccaccgca
      241 acttgttcga aggcctggat gcggccccgg atgtggcgga agacagcgtg ctgatcgtcg
      301 ccgaccggga ccggcccgga cccgggccac acggcgtcgc gctgatctgg ctgaaggcgg
      361 tcggaatcga tctgtcgacg ccgcatcgcg ggattgccat cgtcaaacgc gcagcggccg
      421 actcgctggc ggccgccggc agcatcggcg tttaccggcc ggtgacgccg cgcgccgtgc
      481 agcgctagca gcctgccgga ctcagctccg acaaactcct gggctcgacg ggatgcgcga
      541 ccagtttgca ttgatcgccg gcgccaccgg cctgatcggg cgccgtattg cggcgcatct
      601 ccatcggctc ggctgggagg tcatcggcct gtgccgcagg ccggtaccgg gcggcgccgt
      661 gcggctgatc agcgtcgatc tgaccgatgc cggcgactgc cgcagcaagc tcgcggagct
      721 gggcaacgtc acccacgtct tctacgcggc gcgctacgat catcccgagg gcgccgcgga
      781 atcggtcgag atcaacgcgg cgatgctggc caatctggtg gaagcggtcg agccggcggc
      841 ggccggcttg caccacatcc acgccgtgca cggcaccaag tattacggcc atcacctcgg
      901 gcccctggcc gtgccggcac gcgaggacga tccgcgtgcg ccggcgccca acttctatta
      961 cgcgcaggaa gactacctgc gcgagcgtca gcgcgggaaa cgctggactt acagcacgtc
     1021 gcggccgcac acgtactgcg atgcctcgtg ggatgaagcg cgcaacatcg ggctgctcat
     1081 cgcggtctat gcggccgtcc tgcgcgagct gggcctgccg ctcggctacc cgggaaccgc
     1141 caggagtttt cgcgttgcca cgcagttcac ggcactaccg ttgctcgccc gcgctctggc
     1201 gtggatggcg accgagccgc gttgcgcaaa ccaggcatac aatatcgtta acggcgatgc
     1261 cccgcgctgg tcggaactgt ggccgcgttt cgccgcgtat ttcggcatgc agacgggacc
     1321 ggagcagccc gtccgtctcg ccgacgccat ggcggacaag gagggcgtgt ggcaggccgt
     1381 gatccggaag cacgggttgc gaccgaccgc gctgcagtcg ctcgtgcggt ggtcgtatgg
     1441 caattatgta ttcgcgccgg aatgggacat aatttccgac ggatccaagg cgcgcggcca
     1501 cggctttgcc gagaccgtgg acagtgcaaa gatgtttctg gaaatattcg agtttttgcg
     1561 tgccgagaag atcgttccat aagagcgcct aacatgatga aacacacgct gcgctgtcac
     1621 ggttttggca acgcgtgcaa gcgacacgat cagtccccgc aaggggcatg cgtcgagcgg
     1681 gcggccgcgg gccaaaaccg cgccagccca cgaggggttg cggccagaat cgccgttggc
     1741 tttgtcgctc ggcttgccaa tatcgacata ttggctgcac ctcgcttcgc gctatcgacg
     1801 attctggctc gcaccgcacg cgcgtttcat catgttaggc gctctaggag ggaagccgtg
     1861 aatgtcatta cgtcccgccg cgtgctgctg gctgcgctgc ttgccgctgg ggttgcgcag
     1921 gcccagggtt atcctgccaa gaccatccgc tggatcgtgc cgtggccgcc cgggggaggc
     1981 gcggacgtgc tctcgcgcat gttgagcccc cacctatcgg aggcgctcaa gcagcagatc
     2041 gtgatcgaca atcgcggcgg ggccgccggc aacatcggcg ccgagatggc cgccaagtcg
     2101 ccgccggacg gctataccat cgcgttcgcc tactccggca cccacgcgat caaccccagc
     2161 atctaccgca agatgccgtt caaggagagc gatttcgcac ctatcatctg gctttcctcc
     2221 gtgccgcagg tgctggtcgt gcatccgtcg ctgccggtga agacggtgaa ggaactcgtc
     2281 gcgctggcga agaagcgccc gggcgagctc acctacggct caagcgggag cggcgcgttc
     2341 aaccacctta ccggcgcact cttcggtcaa atgaccggca cccggctcgt gcacgtgccg
     2401 tacaagggcg gggggccggc ggcggtggcg ctgctctctg gcgagatcac gatgatcctg
     2461 ggagagcccg catccctcgt cggtttcatc aagaccgaaa gggtgcgcgc gctcgcggtg
     2521 accggcgcga aacggacgcc gggcctgccc aacctgccga cgatcgccga gtcgggggtc
     2581 gccggctacg aggcgacgtc gtggaacggc atgctggcac cggcgggaac gccggccgac
     2641 atcatcaagc ggctcaacgc cgagttcaac cgcatcatcg gcacgccgga catgaaaaag
     2701 cgcatgctcg acaacggcta cgagccggtg ggcggatcgc cggagagatt cggagagttg
     2761 atccgcgccg aaatcgcgaa gtgggcaccg gtggtcgcgg ctgcgggcgt cagggtggat
     2821 tgatcgcgag ggagctggat agatggcgag cgggcgcatg ggactgctgg tcgcggggtt
     2881 caattacgcg accgtggcgg gggacgagtt caacgattgg tacgacaccg agcacatccc
     2941 cgagcgcgaa cgcatcaagg gcttcgtgaa ctgcgagcgc tggctgggcg cggacgatcc
     3001 cacggtgtcg atcgcgacct acgatctcga gtcgctcgcg gtgctgcaga gccccggcta
     3061 ccgcgcgatc gcgggcgcca acctgtcgcc ctggtcgagg cgcatcaccg gcagatgcca
     3121 gcgcatctgc cgcttcgagg ccgaacagat ttcccccggc gatcgagccg cgccgcgcga
     3181 cgcgggcggg ttgctgctgt tcgcgatgaa cgccgccggc gaattcgagg cggaattcaa
     3241 cgcctggtac gacgaggaac acattccgcg gctctcgaag gtgggggggt gcctgtgcgc
     3301 gcggcgcttc cggacgacga gggccatcag caacggcaac caccgctacc tggcgctcta
     3361 ccatcttgca acgccggagg tatgctcgtc cccggcatgg aaagcggcgg cggaaacgcc
     3421 gtggacgctt aagctgcggc cgcacatgaa cgaccggatg cgactggtgc tccggcgcta
     3481 ccaaaggaag gcttgacggt cctggaggag aacgagcgat ggcaaccatt caaacgcgca
     3541 gtcttgcatg caccgcgctt cttgcgctcg ccccggcgct cgccgccgtc gccgacgcgg
     3601 ccgagtatcc ggcgcggccg atccgctacg tcgtcgcgtt cgcgcccggc ggcatcaacg
     3661 acatcctggc gcggatcgtg ggccagaaac tgaacgaaag ctgggggcag cccgtcatcg
     3721 tggacaaccg ccccggcgcg ggcggcaacc tcgcggcgga gctggtctcg aaaagcactc
     3781 ccgatggcta caccttcatg aacatcagca ctgcgcacac gatctctcaa acgctttatt
     3841 ccaagcttaa ctacagcctc gaacgcgatc ttgcgccggt ggtcgtactc ggttcctcac
     3901 cgctcatcat ggtggtccat cccggcattc cggcgaagaa cgtgcccgag ctcgtcgcgt
     3961 gggcgaagaa aaaccgtttg atctacgcct cgggtggagt cggggtgatc agtcacctct
     4021 cgatggagat gtttaaggtg gccgcgggga tcgacgcgac gcacgtgccc tacaaggggg
     4081 tgggaccggc cgtacccgat ctcatctcgg gacaggcgca cgtgatgttc aacgcggttc
     4141 cggagctgtt tccgttcgcg aaagggggac gcctgcggat catcgccacg ctaaccgaga
     4201 agcgccatcc gttcatgccg gaggtcccga cgatgattga gcagggctac aaggaattcg
     4261 tcatgggtaa ctggacggga atcgtcgcgc cggtcaagac ctcgaagaat gtggtgaacc
     4321 ggctcgccgc ggagattacg cgcatcatcc gttcgcccga gaccagcaag cgcctcgtgg
     4381 agatgggcgt cgacccgctg ggcggaacgc cggaggagtt cggcaagctc atccgttccg
     4441 aggtcgcgcg cttcggcaag gccgtgaggg attcgggcgc gaaggccgat tagtgccgtt
     4501 ccaactcaag cactaatgac cacgaggatc cctttgctga atatcgtgcc tcgatgacgc
     4561 atcacagtaa gctcggaata gttggagcgg cactaggagg aggccccacg cgcatgttcg
     4621 cacgcttcgc agtcatcagc agcctcgcgc tcgccgcggc gctgtcgtcg ccggcgggtg
     4681 ctcagcagtt tccgacgcgt ccgatccgct ggatcgtgcc ggtgccaccc ggcgccacga
     4741 ccgacatcat ctcgcggctg atcggcggca agatgagcga agcctggggc cagcaggtgg
     4801 tggtggacaa ccgcaccggc ggggcgttcg tgatcggcac cgacatcgtc gccaagtcca
     4861 atccggacgg ccacaccatt gggatctttc tcacgccgca cgcggtgaac ccgttcgtgc
     4921 tgaagaactt gccgtacgac accatcaggg actttcagcc gatcacgctc gtcgccatcg
     4981 tccccggcgt gatggtcacg aaccccaatg tgccggtggc gagcctcaag gacgtgatcg
     5041 agctcgccag gacgaagccg ggccagctca actacggctc gcccggtccg atgacctcgg
     5101 ggcatctgtc catggagctc ctgaacctga cggccgggat caagatcctg cacgttccct
     5161 acaagggtgg cgcgccggcg atcttggatc tcctcggcgg gcagatccag ttcgtgatcc
     5221 tcggcccgcc caccgtcatg gggcacctca aggccgggcg cttgaaggcg atcgccacca
     5281 cggccgcaaa gcgctcgccg ggcctgccga acacgccgac cttcgccgag tcggggctgc
     5341 ccggctacga tacctatgaa tggtacggtc tcttcgcccc ggcgcggatc ccgcgcgcgg
     5401 cgctggcgaa gctcaacggc gaggtgaatc gcatcatcgc gctgcccgat atacgagagc
     5461 gcatgctcgg gctgggggcg atcccgagcg gcaatagcac cgaggaattc gccgcgttcg
     5521 tcaagggcga gatgaaccgg tggggcaagg tcgcgcagca ggtagggctg cggcccgact
     5581 gaacgggatg aacgacaatc agcgcccgcc cggacggcgc gcaggcgctg tctgggtcgg
     5641 catcgacacc ggcggcacct tcaccgatct cgtcgcagtc gaactcgaaa gcggccggca
     5701 ccattaccac aaggtgccga ccacgacgga ggatcccgcg cgcggcatcc tggatggcat
     5761 cgcggagctg ctcgaccgca accggctggc gcgcgccgac gtggcgtttc tggtcctcgg
     5821 cacgacgctc gctacgaacg cggtgctgga aggcaagtgg gcgccgaccg ggctcatcac
     5881 caccgcgggt tttcgcgacg tgctcgagct cgcgcgccag cgccgcccgc attacttcaa
     5941 cctcgacatc ccgaagccga tgccgccggc cacgcgcgac tgccgcatcg aggtcggcgg
     6001 ccgcattgcg cacgacggtt ccgaagtggc gccgctcgcg gaggacgaag tgcgccgcgc
     6061 cgtcgagcgg ctgaagcgga agaaggtgga ggcggtcgca gtgtgctttc tccatgccta
     6121 cgccaacccg gcgcacgaag agcgggcaaa ggcgctggta gccgggctct ggcccggcgc
     6181 ctatctctgc agctcgagcg aggtgctcgc cgagtttcgt gaattcgagc gcttcgccac
     6241 cgccacggtg aacgcgagcc tgatgccgat catggaccgc tacctcgagc gcttcggccg
     6301 cggcgtggcg gatctcggca tccggccggt cccgtgcgtc atgcagtcga atggcggcgc
     6361 ggtgtcgccc ggcgcggtga ggcgactccc cgtcaacacg ttcttttcgg gaccggcggg
     6421 cggcgtcatc ggggcgtcgg gcctgggcga gcgccttggc cttcccaacc tcatcacctt
     6481 cgacatgggg ggcacctcga ctgatgtctg cctgatccgc gacggggagc ccgcgcaaaa
     6541 gagcgagcgc ctgatgggcg gctttccggt gcgcacgcgc gcgcttgaca tccacaccat
     6601 cggcgccggt ggcggctcca tcgcctggag ggacgcgggc gggctgctca aggtcggacc
     6661 gcagagcgcc ggcgcgcatc ccgggccggc agcctacggc cgcggcggga cccaggccac
     6721 ggtgactgac gccaacgtgg tgctgggccg gctcaatccc cggcggctcc tcggcggccg
     6781 catgagcatg catccggaga aggcgcgcgc agtgatggac gagctcgctg ggcggctggg
     6841 agtggacacg gcgcgcgccg cggcgggcgt gatcgagatc gtcaatgtca acatgatggg
     6901 cgcggtgcgg gtgatttccg tcgagcaggg cgaggacccg cgcgacttca cgctcgtcgc
     6961 cttcggaggc gcggggccgc tgcacgcggc ggacatcgcg cgcaacatgg gtatcgtgaa
     7021 ggttctggtg ccgccgcggc cggggctgct ctcggcgatc gggctgctcc acgccgacgt
     7081 acgcggcgac tttagtctca cgcggctgtt gcgcgcggcg tcggagaacc tgcaggcgct
     7141 caactgggga ttcgaagagc tgaggcgccg cggcgcgaac tggctcgaag gcgaaggcga
     7201 ggcgcgcaca ggtttcaact ggttcgcgga cctgcgctat tccggacaga atttcgagct
     7261 gatcatggag ctgaagagcg gcgggctcga cgaggcggcg ctctcgaggc tcgtcaattc
     7321 cttccatcgc cgccacaagg acagttacgg ctacgatatg cgcggccagc cggtcgagat
     7381 cgtgaacctc cgcctgatgg tgacggcaaa acgccgcatt ccgccccacg agagcatccg
     7441 gatgaagcgg ggcgatcccc ggcaggcgct cctcgaacgc cgccgggtgt ggtttcccga
     7501 aacagggttt gtcgcggcac cggtttatga gcgcgaccgg ctgcccgccg actgccgcat
     7561 cacggggccg gcggtcatcg agcagatgga caccaccacg gtggtgccgc ccaaagcccg
     7621 gttgaggagc gaccggctcg gctacctgca ccttgagctt gagcccgtgg aacagaaacg
     7681 gggcgcaaca tgggctgcgg cgtagacccg atccgggccg aagtcgtggc gcgctttctc
     7741 ctcgcagcgg cggaagagat gggcgcaacc ctcatgcgca ccgcgttctc gcccaacatc
     7801 aaggagcgcg cggactgctc gaccgccatc ttcgacgccg ccggcgaggt ggtcgcgctg
     7861 gcgcagcgcg tgccgatcca cctggggtcg atggtcggcg cggtggacga gatccgcagg
     7921 cgctaccggg agcaggacat ccgtcccgga gacatgttca tcgcgaacga tccctacacc
     7981 ggcggcggca cgcatctgcc cgacatcaac atcatcgcgc cggtattcct gggcaagcgc
     8041 atcgtcgctt tcgtcgcgaa catcgcacac cacgccgacg tcggcggcat ggtccccggc
     8101 tcggaagcgg cggtctgcaa gtcgatcttc caggagggca tccgcatccc gcccgtgcgc
     8161 atccagcgcg cggggaagtt gaaccgcgac gtgcttgagc tgatcctgct caactcccgc
     8221 acgcccgggg agcgcgtggg tgatctgcag gcgcagtttg cggcgaacac ggtgggcatg
     8281 cgcagcgtgc ttcagctctt cgatcgctac gggccgaagc agacgcaagc ggcggtcgcc
     8341 gcctatctcg acttcaccga gaagcgcttc cgcgcggcga tcgagcggct gcccaaggga
     8401 cgctatgagg ccgaggactt tctcgatggc gatgccgaag gcgaagtgtg ccgcatccgc
     8461 ctcgcgctca cggtgggcaa aggccggctc catttcgact tcaccggctc cggtccgcag
     8521 ctcgcagccg cgcgcaacat accgtaccgc gcgctgcttg ccacggttta cacggtggcg
     8581 aagagcctgc tcgatccgga ggtgcccgcc aacgccggct actaccgcac cctgaccatc
     8641 aaggcgccgc ccggctgcgt ggtgggcccg gtgccgccgg cggcggtcgg ctgccgctcg
     8701 atttccgcga gcgtgctggg cgatgtcatc gcccaggcgc tctcccgcgc catgccggag
     8761 aaggcgctgg cgggctgcgg accgcatcag ctctacgtgc tctcgggcac ggatccgcgc
     8821 accggggcct atttcgtgaa ctacgagacg ctcgccggcg gttacggggc gcgcgccggc
     8881 cgcgacggcg tggacggcgt gcgcgtgcac gcgtccggct cctcgaacct cccggtggag
     8941 gcgctggaac acgcctaccc gttccgcgtc gagcgctacg cgctgcggga cgggtcgggc
     9001 gggacggggc ggtaccgcgg cggcgccggc gtcgagcgcg attaccgcgt gctggccgac
     9061 ggcattgtcg taagcctctc ttccgagcgc cagcatgtcc cggcgaaggg catgatgggc
     9121 ggacgggacg gcgcgaccgg cgccttcgtc ctcaatcccg ggacagcgga ggagcgcgcc
     9181 ctgccggcgg cggcggcgga cgtgcggctg ccgcacggga gtctcttccg gatccgcacg
     9241 ccgggcggcg ggggctacgg cgatccgcgc gggcgcgccc gcggcgcggt cgagcgcgac
     9301 ctgcgcgagg aacggaccac gccggcacag gcgcgggagc tttgcggctg gggagagaag
     9361 acatgagccg gccacgcgaa acggatgcga tcggctcagc ccgggcgaac tacccggccc
     9421 tcgagcgctg gacttatctc gatgtcgccg ggcgcggtgt gctctcgcgc gcggtgcgcg
     9481 ccgcgctcga cgcgcatctc gacgaacgca tgctgaacgg cgcggacaag cagaaattct
     9541 tcgagttgat cgagaatacg cgcgccgggt tcgcgcggct gatcggcgcc gcgcccgagg
     9601 aggtcgcctt caccaagaac atctccgagg gtctcaacat gatcgccacc gccttccact
     9661 ggcgccgcgg cgacaacgtg atcctgtgtc ccgggctcga gcacccgaac aacgtctatc
     9721 catggctcaa catgcgccgc tacgggctcg aggtgcgggc ggtcgccccg cgcgcgggcc
     9781 acattccggt ggacgagatc gcggcgagga tcgacggcgg cacgcgcgtg gtgacggtct
     9841 ccaccgtcac cttcgcgccg ggtttccgca ccgacgtgga cgcgctcggc aacgtctgcc
     9901 gcgagcgcgg cgtgctgctg ctggtggacg ccgcgcagtc ggtgggcgtg ctccataccg
     9961 acgtcgggcg ctcgaagatc gacgcgctcg cggtctccac cgcgaagggg ctgctcggcc
    10021 tctacgggat gggttttctc tattgccgga gcgactgggc cgagcggctc gcgccggcct
    10081 atcttgcgcg cttcggcgtg gacctcggcg ccgcgcacga ggcgacgatg gggggcgacg
    10141 aataccgcct gatgccgggc gcgcgccgct tcgacctcgg caactacaac ttcgcggccg
    10201 ccgcggcggt ggaggcgtca atgcgggagc tcgccgcggt cggcaccgag cgcatcgagc
    10261 ggcacgcggt cgggctcgcg cacgcgctcg cccgggggtg tctcgatctg gggctgccgg
    10321 tcgccggcgg cgcgccgggg ccgcatctcg cccacatcgt caccgtgggc gagatgggcg
    10381 ccagccacta cggctcggag gacgagcgca tcaaccgcct ccacgaccat ctcgtcgcca
    10441 accg
//
LOCUS       DBNGWZ010000537         5611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_202007, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000537 DBNGWZ010000000
VERSION     DBNGWZ010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_202007"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="AC16CX_16745"
     CDS             <1..1285
                     /locus_tag="AC16CX_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3014192.1"
                     /translation="TPREFLERCLEIIAARDKSVRAFVTLNTVAARKSADASTKRYKA
                     GRPLSPVDGMPVAVKDIIATADMPTQMNCPAFKGWQSGQDAACVAALRKGGAVIIGKT
                     VTTEFAIGYSGPTTNPFDPERTPGGSSSGSAAAVGAGMVPAALGTQTQASTLRPASYC
                     GAVGFKPTIGTLHTGGIHPLSTTCDHLGIIAGTLEDAWRVASQISLGIGSPGSLFLSG
                     AGTEPPAPRKPRKLIRLYTRGWTEIDSDTEDTFNETIAALQFAGVTIASRNNDSAVAK
                     LEEELNNGVDGALAIVAYEMKWPYEDYIARFGKIIGARIHGLIEQARGMSPADYEALL
                     ANRRRVRHLVHETAGGAEGYITLAASGPAILGLEYTGSRTFLVHGSWLGLPAFALPLL
                     EINGLPLGIQLIGAPGADGALCAAANWFMKELNSL"
     gene            1410..2390
                     /locus_tag="AC16CX_16750"
     CDS             1410..2390
                     /locus_tag="AC16CX_16750"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014193.1"
                     /translation="MKKSIFVKALTLACGITGLCPSIALTQAYPSKPIRLIVPFPAGG
                     GVDFIGRIAGRGMSERLGQQVVIENRAGANAIVGLEVLKSSPPDGYTIAAASAGPLAV
                     NPFIYAKLPHDTLKDFTQIANMVNFPLLMVTHPSLPVKSVKELIALARSKPGQVTYAS
                     PGAGNSSHLAGELFNSMAKVNILHVPYKGMAPAVVSVVSGEAPILYASIPPILPHVRI
                     GRVRALGVCNLKRVQSLPEFPTVAESGLPGYEAYAWAGMIGPANMPRDVVMRLNKEIV
                     ATLNQKDVVDRMLAEGTVPTPSSPEEFTAYLKSELKKWGAVVKLAGIKPE"
     gene            complement(2526..3581)
                     /locus_tag="AC16CX_16755"
     CDS             complement(2526..3581)
                     /locus_tag="AC16CX_16755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3014194.1"
                     /translation="MLKIGILLGDDIGLEVVPEAVKVMAAAAKKTGLEVQWSGHPIGK
                     KGHELHGHTFPQVTIEALGKLDGWLLGPIGHGAYPRDDPTWIMPPVRKRFELFASIKP
                     VKSYPNIPSVHKDVDIVFLREVTEGMQSSDTVVMGAGEFRPNDEITIASRVITRKGSN
                     RIARAAFEIARTRSRRKVTSVHKEPVYRLACGMFAEECRKVAKEFPDVAFEEVLVDGF
                     TMKLMMNPWQYDVVVTTNQFGDILTDQGAGLVGGLGLAPGLCIGEHQAMAQATHGSAP
                     DIAGKRVANPYALIMSGKMLLEWLGRKRNEPKAIEAAQRIDSAMDKVIAEAKHLTPDL
                     GGKASTMEMGDAIAAAV"
     gene            complement(3670..4626)
                     /locus_tag="AC16CX_16760"
     CDS             complement(3670..4626)
                     /locus_tag="AC16CX_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014195.1"
                     /translation="MLRFVGCLLFAAFAFAPSGWAQTWPAKPIRLVVNFPAGGTTDIM
                     ARAFAPRLSETLGQPIVIENRGGAGGNVGLELVVRAAPDGYTLLASSSSPIVVGPHLY
                     KLNFNVARDLVPIVPLGRILTILVVRPSLPVRSVPELIAHARANPGKLNYGSVGSGST
                     LHIHAERMLYAAKIQATHVPYKGAALLVNALLGDQVDFAFDSGLTIPHIRAGKLRLLA
                     VVGPGRTPLFPDAPTMTEMGVEIEGDSVFGVYAPTGTPRNIVARLNREIGRIMQTAEA
                     RAALATFAAEVVTASPEEFAARQQRDRERFGAYIREANIRVD"
     gene            complement(4748..>5611)
                     /locus_tag="AC16CX_16765"
     CDS             complement(4748..>5611)
                     /locus_tag="AC16CX_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3014196.1"
                     /translation="CDGWIMGPIGHAAYPRNDPTWVMPPVRKRFQLFASIKPVKSYPN
                     IQSVHKGVDIVFLREVNEGMMSSSVVMAGSGEFRPNDEISIGMRVITRKGASRVAREA
                     FEIARARTRRKVTAVHKEPVYRVCCGMFAEECRKVAKEFPDVKFDEVNIDSMAMKLVL
                     NPQPYDVIVTTNQFGDILTDEGAGLVGGLGLAPGLCAGERRAMAQATHGSAPDIAGKN
                     LANPYAMIMSGKMLIEWLGRKRNEPKATQAAKLIDAAMDKVIAEAKHLTVDLGGKAGT
                     VEMGDAVAAAI"
BASE COUNT          957 a         1893 c         1743 g         1018 t
ORIGIN      
        1 cacgccgcga gagttccttg agcgctgcct tgaaatcatt gccgcgcgtg ataaatcagt
       61 cagggcattt gtcacgctca acaccgtcgc cgcgcggaag agcgcggacg cgtcgacgaa
      121 gcgctacaag gcgggcaggc cgctttcgcc ggtggacggc atgccggtcg cggtgaagga
      181 catcatcgcc accgccgaca tgccgacgca gatgaactgc ccggcgttca aggggtggca
      241 gtccggccag gacgcagcat gcgtcgcggc attgagaaaa ggcggcgcgg tgatcatcgg
      301 caagaccgtg accaccgagt tcgcgatcgg ttattccgga ccgacaacca atccgttcga
      361 ccctgagcgc acgcccggcg gttcctcgag cggctcggcg gcggcggtcg gcgccggcat
      421 ggtgccggcc gctctcggca cgcaaacgca ggcttccacg ctgcgacccg cgtcatattg
      481 cggggcggtc ggcttcaagc ccacgatcgg cacgctgcat accggcggca tccatcccct
      541 gtccacgacc tgcgaccatc tcggcatcat cgccggcacg ctggaggacg cgtggcgcgt
      601 cgcctcgcag atctcgctcg gcatcggcag ccccggcagc ctgttccttt ccggcgcggg
      661 gacggaaccg ccggcaccga ggaagccgcg caaactcatc cgcctctata cccgcggctg
      721 gaccgagatc gacagcgaca cagaggatac cttcaatgaa accatagccg ccctccagtt
      781 tgccggtgtg acgatcgcga gccgcaacaa cgattcagcc gtcgcaaaac tggaggagga
      841 gttgaataac ggcgtggacg gcgcgcttgc catcgtcgcc tacgagatga agtggccgta
      901 cgaggactat atcgcacgct tcggcaagat catcggcgcg cgcatccacg gcctgatcga
      961 gcaggcgcgc ggaatgtcgc ccgccgacta tgaagcattg ctggcgaacc gcaggcgggt
     1021 gcggcacctc gtgcacgaga cggcaggcgg cgctgaaggc tacatcacgc tcgccgcctc
     1081 cggcccggcg atcctcgggc tggaatacac cggcagccgc acgttcctcg tccatggctc
     1141 gtggctcgga ttgccggcgt tcgccctgcc gttgttggag atcaatggac tgccgctcgg
     1201 catccagctc atcggcgcac cgggcgcgga cggcgcgctg tgcgcggccg cgaactggtt
     1261 catgaaggaa ctgaattccc tatgatctcc gcagttttgg tttcttgatc ttcgtcaaca
     1321 tttgtctgca ttcacagccg gcctgatcac ccaagcggtc cgggcgggaa gtatacttgc
     1381 cctcacctga acaacgaccg gaggaggcga tgaagaaaag tatcttcgtc aaagcgctga
     1441 cgcttgcgtg cggtatcacg ggactttgcc cctcgattgc gctcactcaa gcctatccca
     1501 gcaagccgat ccggctgatc gtgccgttcc ccgccggcgg cggcgtggat ttcatcggcc
     1561 gcatcgccgg caggggcatg tcggaacgcc tgggccagca ggtggtcatc gagaaccgcg
     1621 cgggcgccaa cgcgatcgtg ggcctggaag ttctcaagtc ctccccaccc gacggctaca
     1681 ccatcgcggc cgcctccgcc ggcccgctgg cggtgaaccc gttcatctat gccaagctgc
     1741 cccacgatac gctgaaggac ttcacccaga tcgccaacat ggtgaatttc ccgctgctga
     1801 tggtcacgca tccctcgctg ccggtgaaga gcgtcaagga gctcatcgcg ctcgccaggt
     1861 cgaagccggg ccaggtcacc tatgcctcgc ccggagccgg caattcgtcg cacctcgccg
     1921 gcgagctgtt caactccatg gccaaggtga acatacttca tgtgccctac aagggaatgg
     1981 cgcccgccgt ggtgtccgtg gtatccggag aagccccgat cctctacgcc agcatcccgc
     2041 cgatacttcc ccacgtgcgc atcggcaggg tcagggccct gggcgtgtgc aacctcaagc
     2101 gcgtccagtc gctgccggaa ttcccaacgg tggccgagtc cggcctgccg ggatacgaag
     2161 cctacgcttg ggccggcatg atcgggcccg ccaacatgcc gcgcgatgtc gtcatgcgcc
     2221 tgaacaagga gatcgtcgcc acgctgaacc agaaggatgt ggtcgaccgc atgcttgccg
     2281 agggcaccgt gcccacaccc tcgagccccg aggaattcac cgcctacctg aagtcggagc
     2341 tgaagaagtg gggcgcggtg gtgaagctgg cgggcatcaa gccggagtga cgcggcgcgt
     2401 cactccaaga aggcgaaagt cgaaaggcga atggcgaaag gactgctcag gtcccagcac
     2461 tgcgggcgct ggaatggatc tactttcgcc tttcgacttc cgcccttcgc cttcgtcagc
     2521 ggcggtcaca ccgccgccgc gatggcgtcg cccatctcca tagtactggc tttcccgcct
     2581 aagtcgggcg tgaggtgctt tgcctccgcg atgaccttgt ccatcgccga gtcgatgcgc
     2641 tgtgcggcct cgatcgcctt cggctcgttg cgcttgcgcc ccagccactc gagcagcatc
     2701 tttcccgaca tgatcagcgc ataggggttg gcgacgcgct ttcccgcgat gtcgggcgcc
     2761 gagccgtggg tcgcctgcgc catcgcctgg tgctcgccga tgcacagtcc cggcgcaaga
     2821 cccagcccgc ccacgaggcc cgcgccctgg tcggtcaata tgtcgccgaa ctggttggtg
     2881 gtaaccacca cgtcgtactg ccagggattc atcatcagct tcattgtgaa gccgtccacc
     2941 agcacttcct cgaacgccac gtccggaaac tccttcgcca ctttgcggca ttcctcggca
     3001 aacatcccgc aggcgagccg gtagaccggc tccttgtgca ccgaggtcac cttgcggcgg
     3061 ctgcgcgtgc gcgcgatctc gaacgccgcg cgggcgatgc ggttggagcc tttgcgcgtg
     3121 atcacgcgcg aggcgatcgt gatctcgtcg ttcggccgga actcgcctgc gcccattacg
     3181 acggtatcgg aggactgcat cccctcggtc acctcgcgca ggaacacgat gtccacgtcc
     3241 ttgtgcaccg atgggatgtt gggataggac tttaccggct tgatgctcgc gaacagctcg
     3301 aagcgcttgc gcacgggggg catgatccag gtcgggtcgt cgcggggata ggcgccgtgc
     3361 ccgatcggcc ccagcagcca gccgtcgagc ttcccgagcg cctcgatcgt cacctgcggg
     3421 aaggtgtgtc cgtgcagctc gtgccccttt ttcccgatcg ggtggccgct ccactgcacc
     3481 tcaagccccg tcttcttggc ggcggccgcc atcactttga cggcctcggg gacgacttcc
     3541 agaccgatgt catcccccag cagaatgccg attttcaaca cgaccgactt ctccaatcct
     3601 aagggccatt cgtcacgagg ttaccagtgg gccggggctc acgttggata gaagcgattc
     3661 cgcgcccgct cagtcaacgc ggatattggc ctcgcgtatg tacgcgccaa agcgctcgcg
     3721 gtcgcgttgc tgtctcgctg cgaattcttc cggtgaagcg gtgaccacct cggccgcgaa
     3781 agtggcgagc gcggcgcgtg cctccgcagt ctgcatgatg cggccgatct cgcggttcag
     3841 ccgcgcgacg atgttacgcg gcgtgccggt cggcgcgtat accccgaaca cggaatcgcc
     3901 ttcgatctcc acccccatct ccgtcatggt cggtgcatcg gggaagagcg gcgtgcgccc
     3961 ggggcccacg accgcgagca ggcgcagctt gccggccctg atatgcggga tcgtcaggcc
     4021 cgagtcgaaa gcgaagtcga cctggtcgcc gagcagggcg ttgaccagca gcgccgcgcc
     4081 cttgtacggc acgtgggtcg cctggatttt cgccgcgtag agcattcttt cggcgtggat
     4141 gtgcagcgtg ctgccgctgc ccaccgatcc gtagttgagc ttgccgggat tggcccgcgc
     4201 gtgggcaatc agttccggca cgctgcgcac cgggaggctc gggcgcacca ccaggatggt
     4261 caggatgcgg cccagcggca cgatcggcac caggtcacgg gccacgttga aattgagctt
     4321 gtaaaggtgc ggacccacga cgatcggact gctggatgac gcgagcaggg tatagccgtc
     4381 cggggctgcc ctgaccacca gttcgagacc gacgttgccg cccgcgccac cgcggttttc
     4441 aatcacgatc ggctgaccca gcgtttcgga aagcctcggc gcaaatgcgc gggccatgat
     4501 gtcggtggtg ccgccggccg gaaagttgac cacgagccgg atcggtttgg caggccatgt
     4561 ctgcgcccag ccggacggtg cgaatgcgaa tgccgcaaaa agaagacagc cgacaaaccg
     4621 taacatgttc gcctcctgca cgagtcgatg ggaccactgt ttcgcgccgc gacgctgctg
     4681 acgctcatcc agccatcgtc actatcaatc gtcaccctta tccgtcaccc ttgatcatca
     4741 ctatttttta gatggcagct gcgacagcat cgcccatctc gaccgtgccc gccttgccgc
     4801 caaggtcgac ggtaaggtgc ttcgcctcgg cgatcacctt gtccatcgcg gcgtcgatga
     4861 gcttcgccgc ctgcgtcgcc ttcggctcat tgcgcttgcg gcccagccac tcgatgagca
     4921 tcttgcccga catgatcatg gcgtagggat tggcgaggtt tttccccgcg atgtcgggcg
     4981 ccgagccatg ggtcgcctgc gccatcgccc gccgctcgcc ggcgcacagt cccggcgcca
     5041 gtcccagtcc gcccaccagc cccgcccctt cgtcggtgag gatgtcgccg aactgattgg
     5101 tggtgacgat cacgtcgtac ggctgcgggt tcagaacgag cttcatcgcc atgctgtcaa
     5161 tgttgacttc gtcgaacttg acgtcgggaa actccttcgc cactttgcgg cattcctcgg
     5221 caaacatgcc gcagcagacg cggtacaccg gctccttgtg caccgccgtc accttcctgc
     5281 gcgtgcgcgc gcgcgcgatc tcgaatgctt cccgcgccac ccggcttgcg cccttgcgcg
     5341 tgattacgcg catgccgatg gagatctcgt cgttcgggcg gaattccccg ctgcccgcca
     5401 tgacgacgct cgacgacatc atgccctcgt tcacttcccg caggaatacg atgtccacgc
     5461 ccttgtgcac ggattggatg ttgggatagg acttcacggg cttgatgctg gcgaaaagct
     5521 ggaaacgctt gcgcaccggc ggcatcaccc aggtcgggtc gttgcgcggg taggcggcgt
     5581 gcccgatcgg gcccatgatc cagccgtcgc a
//
LOCUS       DBNGWZ010000538         1622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_327077, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000538 DBNGWZ010000000
VERSION     DBNGWZ010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_327077"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16CX_16770"
     CDS             <1..265
                     /locus_tag="AC16CX_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014197.1"
                     /translation="ANRRQILRAHLAEFTLIGAVSGFVAAAGATGLAYYIAHRFLHLQ
                     YVPDPAVWLYSVAGSALGVAVAGWFGTRRVLAVPPLDVLRAMG"
     gene            378..1583
                     /locus_tag="AC16CX_16775"
     CDS             378..1583
                     /locus_tag="AC16CX_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3014198.1"
                     /translation="MPDILLAILALAVLAALIVLIVRLATQTAAVRMLSQELARALEE
                     KHRSMLSDLHGGLAQQSDRLTSRLSEELTQTRETLHRLQLSLAASLGETTDKLNARID
                     LRLEQIAGKVNERLDEGFKKTNETFVSVMQRLATIDEAQKKIETLTGSVVSLQELLGD
                     KRSRGAFGEVQLEALVRNVLPPAAYEMQYTLPNGARADCVLKLPEPTGLVCVDSKFPL
                     ENYHRMLTPGANDMERAAAQRQFRADVRKHVDDIARKYILPDVTSDGAVMFVPAEAVF
                     AEIHAYHAEVVDDANAKRVWIVSPTTLMAVLNTARAVLKDVETRKQIHVIREALKRLA
                     VEFSRFDERMKDLARHIRQAADDADRIQVTGGKISQQFQRIESAELEPSDVAQLKVVE
                     LRDDGKGAK"
BASE COUNT          311 a          525 c          529 g          257 t
ORIGIN      
        1 ggcgaaccgc aggcagatcc tgcgcgccca cctggccgag ttcacgctca tcggcgcggt
       61 ttcaggcttc gtggcagccg ccggggcgac cgggctcgcg tactacatcg cgcaccgctt
      121 cctgcacctg cagtatgtgc ccgacccggc ggtatggctg tacagtgtcg ccggcagcgc
      181 gctgggcgtg gccgttgcag gctggttcgg cacgcgccgc gtgctcgccg tgccgcctct
      241 cgacgtcttg cgggcgatgg gctgagcgcc ggtgagcggc aagcagtaag cggagagcgg
      301 aaacgacacc gcttttttgt tttctgctca ccgcttaccg ctcacatctt acccgctcct
      361 gctatacttc cgaggcgatg cccgatatcc ttctcgcgat tctggcgctc gccgtccttg
      421 ccgcactcat cgtgctgatc gtgaggctgg cgacccaaac cgccgcggtg aggatgttgt
      481 cccaggaact cgcgcgcgcg ctcgaggaga agcaccgctc gatgctgtcc gacctgcacg
      541 gcgggctcgc gcagcagtcc gaccgcctga cttcgcggct ctccgaagag cttacccaga
      601 cgcgcgagac cctccaccgc ctccaactat cgctcgccgc cagcctcggg gagaccaccg
      661 acaagctgaa tgccagaatc gacctgcggc tggagcagat cgcgggcaaa gtgaacgagc
      721 ggctcgacga gggcttcaaa aagaccaacg agaccttcgt cagcgtgatg cagcgcctcg
      781 ccaccatcga cgaggcgcaa aagaagatcg agacgctcac cggcagcgtg gtgagcttgc
      841 aggagctgct cggggacaag cgctcccgcg gcgccttcgg cgaggtgcag ctcgaagcgc
      901 tggtgagaaa cgtgctgccg ccggcagcct acgagatgca gtacacgctt cccaacggcg
      961 cgcgggcgga ctgcgtgctg aagctgccgg agccgaccgg gctcgtatgc gtggactcca
     1021 agttcccgct cgagaactac cacaggatgc tcacgccggg cgcgaacgac atggagcgcg
     1081 cggccgcgca acgccagttc cgcgccgacg tcaggaaaca cgtggacgac atcgccagga
     1141 aatatatcct gccggacgtg acctcggacg gggcggtgat gttcgtgccg gcggaggcgg
     1201 tgttcgccga aatacacgcc tatcatgccg aggtcgtgga cgatgccaat gcaaaacgcg
     1261 tctggatcgt atcgccgact acgctgatgg cggtgctcaa taccgcgcgc gcggtgctga
     1321 aagacgtcga aacccgcaaa cagatccatg tcatccgcga agcgctgaag cgcctcgcgg
     1381 tggagttcag ccgcttcgac gaacggatga aggacctggc gcgccacatc cggcaggcgg
     1441 ccgacgacgc ggaccggatc caggtcaccg gtggcaagat ctcccagcag tttcagcgca
     1501 tcgagtcggc cgagctggag ccgtccgatg tagcccagct caaggtcgtc gaattacggg
     1561 acgacggcaa gggtgccaag taatgagtga cgagggtcga gtgacgaata tcaagggaaa
     1621 ag
//
LOCUS       DBNGWZ010000539         5602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_327167, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000539 DBNGWZ010000000
VERSION     DBNGWZ010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_327167"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="AC16CX_16780"
     CDS             complement(<1..1080)
                     /locus_tag="AC16CX_16780"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014199.1"
                     /translation="MIGVEKREYDVLVVGKGNAALCAALSAREQGVTVAILEAATEEE
                     SGGNSRFAGGVMRFAYGTVDDLKRLTDITDEEIAGSDFGTNTREEYLDDLYRLTSYRT
                     DPDLSEILVNRSLDTMAWLCSKGARFNLNFGRQSGLVGGKRKFFGRMPIEATGGGAGL
                     VQNLDAAAKKAGIEVFYETRATSLLYDGERVSGARVQRKGKAAEFHAKSVVLACGGFE
                     ANPEWRTRYLGPGWELAKVRGTRFNVGDGLRMALDIGAAPYGNWSGCHATGWDRYAPE
                     FGDVNVGDQFQKHSYIFGLLINAEGRRFVDEGADFHSFTYAKYGGEVLKQPGQFAWQV
                     FDSKVKHLLRSEYRIKFVTKATANTL"
     gene            complement(1119..2063)
                     /locus_tag="AC16CX_16785"
     CDS             complement(1119..2063)
                     /locus_tag="AC16CX_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014200.1"
                     /translation="MLLSAFAAAPHAAAQNYPTGPIRIVVPFVPGGGTDILARMIAQK
                     LNESWGQPVLVDNRPGASGTVGTAFVAKAAGDGHTLLVMPAGFAGNPSLYKKLPYDHT
                     RDFAPVSWLASGPLTLVVHPSLPVKSVKELIAFARKRPGEINFGSSGAGTLPHLSAEL
                     FNSMSGIKMVHIPYKGAGAAVTDVMAGRVPVYFMNILQSLSLIKAGKLRALGVTTPER
                     TPIAPEIPSIAEAGLNGFDMTNWYGLLAPATTPREVIVKLNAEVKRILQLPELRSRLA
                     QDGMTVVASTPEQFAEFLARETAKFARVIEAAGIKGTL"
     gene            2240..3511
                     /locus_tag="AC16CX_16790"
     CDS             2240..3511
                     /locus_tag="AC16CX_16790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3014201.1"
                     /translation="MSAAAAGWRASSSIPPPLDSTQLKGDRVAMEEIFVANEQQRAIL
                     ATVARFVEQVVTPRAPQLDADPDPEKSFSWEIVEKAHEVGIRTMTLSEKWGGLGADSL
                     TTAMVIEELAKGDLGVSVIMAQTLKIAQIMEKALNDEQQARVLPGFARDPRGVLAIGI
                     TEPDNASNYFLPHPTPFRTSARKAAGGWVVNGMKHFISNGNRASHYLLFAQTEPGKPM
                     AEASTCFLVERGRPGFTIGRVHDKMGERLANNAELVFQDCFLPDENVVGKVGNGFNVL
                     AEFFPQSNAYAGASVLGVAGALHQKAIDWARVRIQGSKPLIEHDGIRAQLAEMRMLID
                     ASRSYIHRACWLADHQQYGWDRTLGALPKAMAAQAAWKIATWTMEIHGGHGYMKELGV
                     EKLVRDAAAFLHSDGVNRTLFLKAANFMFRD"
     gene            3513..4544
                     /locus_tag="AC16CX_16795"
     CDS             3513..4544
                     /locus_tag="AC16CX_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3014202.1"
                     /translation="MRAMVLKGPNTPFELTEVPDPVPGPGEAVARVLACGSGLTIQHI
                     KAGRIAAPFPRIIGHEITGEIVAVGAGVRGLSVGDAVTAYYYLNCGHCRWCLASLEPL
                     CANGRGNVGRNCDGGYAEYIKLPAHIFIKLPEVLDYKAHPAEIGVITDALATPYKVLK
                     RARVRAGETVVVFGAGGGVGIHQVMMAKWARARVIAVDVAADKFDACRRAGADEVVDA
                     SAGDVAQTLLDLTRGEGVDVAIDYVSSTSTLEAGVKALGRRGRLVTLGGAGQPFKVSA
                     LDLLLKEQDLLGSRYVTRSEILESLDLVARGEVWPLVSEIRPLEEAEALHERLEQGKI
                     TGRAALLIK"
     gene            4656..>5602
                     /locus_tag="AC16CX_16800"
     CDS             4656..>5602
                     /locus_tag="AC16CX_16800"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014203.1"
                     /translation="MNLLVRLCIALLPAIWTAGASAQAFPSKPLRVVVAFPAGGPIDI
                     VARTISPALGGILGQQVLVDNRAGANGIIGADHVAKSAPDGYTMILSSPSSIAISPAV
                     YSKMPFDTLRDLATVTLVTTTPELFVVHPSVPAQSVKALVALAKARPGELNIASTGSG
                     GLPHLALELLKTATRIDVVHVPYQGAAPSVAALVGGHAHGMFADLPVLLPHVQAGKLR
                     ALAVASPKRAALLPDLPTMTEQGLPSVEAVNWYGILVQSKTPRDVIAKLHDGLTRTLA
                     DPGVREKLAARGAEPLGTGPDPFGAMLKNEMVRWAKLAQS"
BASE COUNT          960 a         1835 c         1873 g          934 t
ORIGIN      
        1 cagtgtattc gcggtcgctt tcgtcacgaa cttgatgcgg tattccgagc gcagcaggtg
       61 cttcaccttg gagtcgaaca cctgccacgc gaactgcccc ggctgcttga gcacctcccc
      121 gccgtacttg gcgtaggtga acgaatggaa atccgcaccc tcgtccacga agcgcctgcc
      181 ctcggcgttg atcagcagcc cgaagatgta gctgtgcttc tggaactggt cgccgacgtt
      241 gacgtcgccg aactcgggcg cgtagcggtc ccagcctgtg gcgtggcacc ccgaccagtt
      301 accgtacggc gcggcgccga tgtcgagcgc catcctcaga ccgtcgccga cgttgaagcg
      361 cgtgccgcgc accttggcga gctcccagcc cggcccgaga tagcgcgtgc gccattcggg
      421 gttggcctcg aatccgccgc acgcgagcac gaccgatttc gcgtggaact cggcggcctt
      481 gcccttgcgc tggacacgcg cgccggagac gcgctcgccg tcgtagagga gcgaggtggc
      541 gcgcgtttca tagaacacct cgatgccggc cttcttcgcc gcggcgtcga gattttgcac
      601 gaggcccgcg ccgccgcccg tagcctcgat cggcatgcgc ccgaagaact tgcgcttgcc
      661 gccgaccagt cccgactggc ggccgaagtt gagattgaag cgcgcgcctt tcgagcacag
      721 ccaggccatg gtgtcgagac tgcggttcac caggatctcg gagaggtcgg gatcggtgcg
      781 gtagctcgtc agacggtaga gatcgtcgag atattcctcg cgcgtgttgg tgccgaaatc
      841 gctcccggcg atctcctcgt ccgtgatgtc ggtcaggcgc ttgagatcgt ccaccgtccc
      901 gtaggcgaag cgcatcacgc cgccggcgaa acggctgttg ccgcccgatt cctcctccgt
      961 cgcggcctcc agtatggcca ccgtcacgcc ctgctcacgg gcagacaagg ccgcgcacag
     1021 cgccgcgttg cctttcccta ccaccaatac gtcgtattcg cgcttttcaa cgcctatcac
     1081 ttatcaccta tcacttatca cgcttttccg cggggcggct agagcgtccc tttgatcccc
     1141 gcagcttcga tgacgcgtgc gaacttcgcc gtctcgcgcg cgaggaactc cgcgaattgc
     1201 tcgggcgtgc tggccaccac cgtcatgccg tcctgggcga gtcggctcct caactcgggc
     1261 agctgaagga tgcgcttcac ctcagcgttc agcttgacga tcacttcgcg cggcgtcgtg
     1321 gccggcgcga gcagtccata ccagttggtc atgtcgaagc cattcaaccc cgcctccgcg
     1381 atcgagggga tctcaggcgc gatcggggtg cgctcgggcg tggtcacccc gagcgcgcgc
     1441 agcttcccgg ctttgatcag cgaaaggctt tgcaggatgt tcatgaaata aaccgggacg
     1501 cgccccgcca tcacgtcggt caccgcggcg cccgccccct tgtacgggat atggaccatt
     1561 ttgatcccgc tcatcgagtt gaagagttcg gcggataaat ggggcagggt acccgcgccg
     1621 gaggagccga agttgatctc gccgggccgc ttgcgggcaa atgcaatcag ttccttgacc
     1681 gatttcaccg gcagcgacgg atggaccacc agcgtgagcg ggccggaagc aagccaggac
     1741 accggcgcga agtcgcgcgt atggtcgtac ggcagcttct tgtacaggct ggggttgccg
     1801 gcgaagccgg cgggcatgac gagcagggta tgcccgtcgc cagcggcctt ggcgacgaac
     1861 gcggtgccga cggttccgct cgcaccgggg cggttgtcca ccagcaccgg ctggccccac
     1921 gattcgttga gcttctgcgc gatcatccgc gcaaggatgt cggtgccgcc cccgggtacg
     1981 aaaggcacca cgatgcgaat cggccccgtg gggtaattct gcgcggcggc gtgcggggcg
     2041 gcggcgaacg cgctcagcag cacgataacg gaagcgccga ttaacgcggc tggacgcggg
     2101 tgaataggat cccaacgggc tttggccaca gaaaacaccg agagtgctga aaaccgggag
     2161 atacgaatgt taacccaaat cgcgccgccg cattgaggcg cggacgcact tggcgcacaa
     2221 tctgcggcat tccggcagta tgagcgcagc tgcggccgga tggcgcgcat cgtcatcgat
     2281 tccgccgccg ctcgacagca cgcaactaaa aggggaccgc gtcgcaatgg aagaaatctt
     2341 cgtcgccaac gagcagcaac gcgccattct cgcaaccgtt gcgcgtttcg tcgaacaggt
     2401 ggtgacgccg cgggcgccac agctcgacgc ggaccccgac cccgagaaga gcttctcctg
     2461 ggagatcgtc gaaaaggcgc acgaagtcgg cattcgcacc atgacgctat ccgaaaaatg
     2521 gggtgggctc ggcgccgatt cgctcaccac cgccatggtg atcgaggagc tggccaaggg
     2581 cgatctgggt gtctcggtga tcatggccca gacgctcaag atcgcccaga tcatggagaa
     2641 ggcgttgaac gacgagcagc aagcgcgcgt cctgccggga ttcgcgcggg atccgcgcgg
     2701 cgtgctcgcc atcggcatca cggagcccga caacgcctcc aattatttcc ttccccatcc
     2761 gacgccgttc cgcacgagcg cgcggaaggc cgcgggcggc tgggtcgtga acggcatgaa
     2821 gcatttcatc tcgaacggga accgcgccag ccactacctg ctgttcgcgc agaccgagcc
     2881 gggcaagccg atggccgagg cctccacctg ttttctggtg gagcgcggac ggccgggttt
     2941 caccatcggc cgcgtgcacg acaagatggg cgagcgcctg gccaataacg ccgagctcgt
     3001 gttccaggat tgcttcctcc cggatgagaa cgtggtgggc aaggtcggca acggcttcaa
     3061 cgtgctggcg gaatttttcc cgcaatcgaa cgcgtatgcc ggtgcctctg tactcggcgt
     3121 tgccggcgcg ctgcaccaga aggcaatcga ctgggcaagg gtgcggatcc agggcagcaa
     3181 gccgttgatc gagcacgacg gcatccgcgc gcagcttgcg gagatgcgca tgctgattga
     3241 cgcgtcgcgc tcctacatcc accgcgcgtg ctggctcgcc gaccaccagc aatacggctg
     3301 ggaccggacg ctgggcgcgc tgcccaaggc gatggcggcg caagcggcgt ggaaaatcgc
     3361 gacctggacc atggagatcc acggcggcca cggctacatg aaggagctcg gcgtcgagaa
     3421 gctggtgcgc gacgcggccg cgttcctgca ctcggacggc gtcaaccgca cgctcttcct
     3481 caaggccgcc aattttatgt tccgcgactg acatgagagc gatggttctc aaggggccca
     3541 ataccccgtt cgagctgacc gaggttcccg atccggtccc cggcccggga gaggcggtgg
     3601 cgcgcgtgct ggcctgcggt tccggcctca cgatccagca catcaaggcc gggcgcatcg
     3661 ctgccccgtt tccccgcatc atcggccacg agatcaccgg cgagatcgtg gcggtcggcg
     3721 ccggtgtgcg cgggctttcc gtgggcgacg ccgtcacggc gtactactac ctcaactgcg
     3781 gccattgccg ctggtgcctg gcgagcttgg agccgttgtg cgcaaacggg cgcggcaacg
     3841 tcgggcgcaa ctgcgacggc ggctacgccg agtacatcaa gcttcccgcg cacatcttca
     3901 tcaagctgcc ggaggtgctc gactacaagg cgcacccggc cgaaatcgga gtcatcacgg
     3961 acgcgctcgc caccccgtac aaggtgttga agcgcgcgcg ggtaagggcc ggcgaaaccg
     4021 tcgtggtatt cggcgccgga ggcggggtcg gaatccacca ggtcatgatg gcgaagtggg
     4081 cgagggcccg ggtgatcgcg gtggacgtgg ccgcggacaa gttcgacgcc tgccgcaggg
     4141 ccggtgccga cgaggtggtg gatgcctcgg ccggcgatgt ggcgcagacc ctgctcgatc
     4201 tcacccgggg ggaaggcgtc gatgtcgcga tcgattatgt ctccagcact tcgacgctcg
     4261 aagcgggggt gaaggcgctc ggccggcgcg ggcgcctggt gacgctcggc ggcgcgggac
     4321 agccgttcaa ggtttccgct ctcgacctgc tgctcaagga gcaggacctg ctgggcagcc
     4381 gctacgtcac ccgcagcgag atcctggaga gcctcgacct cgtcgcgcgc ggtgaggtct
     4441 ggccgcttgt gagcgagatt cgtccgctcg aggaagccga agcgctccac gagcgattgg
     4501 aacagggcaa gatcaccggg cgggccgcgc tgctgatcaa gtagacgcac ccattgagcc
     4561 tggacgacgc ttgctacaga atgatccctt gcggcgccat gaacacgata tggaattcgg
     4621 gtaaggcgcc ggcgcgcaac tagagggaga gagggatgaa tctgctggtt cgactttgca
     4681 ttgcgctgtt gcctgctatc tggacagccg gcgcctccgc gcaggcgttt ccctccaagc
     4741 cgctgcgcgt ggtcgtcgcg ttcccggcgg gcgggccgat cgacatcgtg gcgcgcacga
     4801 tctcgcccgc gctgggcggg atcctcggcc agcaggtgct ggtggacaac cgcgccggcg
     4861 cgaacggcat catcggcgcc gaccacgtgg cgaagtcggc gcccgacggc tacaccatga
     4921 tcctctcgag cccgagctcg atcgcgatca gcccggcggt ctattccaag atgccgttcg
     4981 acacgctgcg cgatctcgcc accgtcacgc tggtgacgac gacccccgag ctgttcgtgg
     5041 tccatccctc ggttcccgcg cagtcggtga aagctctggt cgcgctggca aaagcgcgtc
     5101 caggcgagct caatatcgct tcgaccggca gcggcgggct gccgcacctc gcgctcgaat
     5161 tgctcaagac cgcgacccgg atcgatgtag tgcacgtgcc gtaccagggc gcggcgccgt
     5221 cggtcgcggc gctggtcggc ggccacgcgc acggcatgtt cgcggacctg ccggtgctgc
     5281 tgccgcacgt gcaggccggg aagctgcgcg cgcttgccgt ggcgagcccg aaacgcgcgg
     5341 cgctcttgcc cgatctcccg acgatgaccg agcaaggtct gccgtcagtg gaggccgtca
     5401 actggtacgg catcctggtt cagtccaaga caccgcgcga cgtgatcgcg aagctgcacg
     5461 atggtctcac caggacgctc gccgatccgg gggtgcggga aaagctcgcg gcacgcggcg
     5521 cggagccgct gggcacgggg ccggacccgt tcggcgcgat gctcaagaac gagatggtgc
     5581 ggtgggcgaa gctggcgcag tc
//
LOCUS       DBNGWZ010000540         4311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1082930, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000540 DBNGWZ010000000
VERSION     DBNGWZ010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1082930"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC16CX_16805"
     CDS             <1..293
                     /locus_tag="AC16CX_16805"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3014204.1"
                     /translation="TGSMFPGKYKHAIFIARHGSWNRTKKVGGDVIVVYLNKDGSVRS
                     QEPFLTGFIQNNAYIGRPVDVQVMKDGSLLVSDDYAGAVYRVTYGGDRVAKR"
     gene            355..939
                     /locus_tag="AC16CX_16810"
     CDS             355..939
                     /locus_tag="AC16CX_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014205.1"
                     /translation="MSDRLLMRSVLAAALVLLPALPAGADSLEARLAQCLACHGESGR
                     SATPGVPSLGGQPAFYITIQLFMFREKLRVVEPMSAMMQGISNDDLRRMADFVARLPP
                     PQPAGGSSDPERLERARALIEQHRCNFCHNRDYSGDQNVPRLAGQREDYLVKALREYK
                     SNARRGYDASMADVLNAISDEQILDLAYFLARFR"
     gene            983..1945
                     /locus_tag="AC16CX_16815"
     CDS             983..1945
                     /locus_tag="AC16CX_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006576125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014206.1"
                     /translation="MRNVHFWALVVALATSSALAQPYPSKPIRLILPYPPGGGSDTIG
                     RPLAQKMSEGLGQQVVVENRGGAGGNIGMELVARAAPDGYTLVFALSPQLAVNVSLYR
                     KIPYDPVKDYQPITLLGSGGYILAVHPSVPAKSVKELIALARSRPGELTYASSGNGSG
                     AHLATELLKTMTGIKLIHVPYKGGGPALVDLLAGQVQILFATQLASAQHIQSGRVRAL
                     AVSTAKRSMGMPDLPTVAEAGVPGYDSGVWYGVLSPAGTPRDIIMKLNGEIVRVLNQP
                     DYRSLLVNNTIEPIGGTPEQFGAFIRSEIKKYAKVVKDAGMRID"
     gene            1980..3047
                     /locus_tag="AC16CX_16820"
     CDS             1980..3047
                     /locus_tag="AC16CX_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3014207.1"
                     /translation="MGIAVVGAGRIGGLRARLAAEHPAVRFMAVSDVEPGRARDLARK
                     TGAQFHTADNAEAIARPEVNAVIISSSEGAHVEPLIQAIGLGKPVLVEKPIALTLADA
                     DRVIRTAEKAGVNVRVGYSRRYKERYLIAKEQIVQGRLGRVVGGAARVFNLRSQATAI
                     LQRNPHATPVVDALTYYVDLMNWMLEGTQVREVFARGQKGVLKDAGFDTEDVSYAILT
                     YADGAVVNLGIAYSLPDQYPARGHAARVEVLGTGGVMILDDDHTDQLMYSEKGVPHVY
                     LPDQPVNMVFLQSGTPGDWALGDFWGPVANETRAWLDHLAVGKPCALATAREARATLE
                     VTLAIEHSAASGRPVSLPLAE"
     gene            3170..4036
                     /locus_tag="AC16CX_16825"
     CDS             3170..4036
                     /locus_tag="AC16CX_16825"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014208.1"
                     /translation="MLVPHAPGGPTDIMARVVGQRLSELWGQTIVVDNRAGAGGNIGT
                     ALVARANPDGYTVLVNSSAFAVNVSLFRKPGYDPKEFEPIINGGVIPNLLFAHPSFPA
                     ATLQELIKIGKTKKLSYASAGMGTTPHLTAERLLKTMAKLDITHVPYNGAGLAANAVV
                     ANEVPIGSAAVTAPAPLIRAGRLKGIAVTSLQRTPALPDIATVAESGFPGYEDYTWIA
                     FFAPRGTPRAVVAKLNAEIASALQVQAIKERLATLGFDFTPNTPEQFGEYLKREVVKW
                     GQVVKESGARVD"
BASE COUNT          797 a         1383 c         1452 g          679 t
ORIGIN      
        1 acacgggaag catgttcccg ggcaagtaca agcacgcgat cttcatcgcg cggcacggct
       61 cgtggaaccg gacgaagaag gtcggcggcg acgtcatcgt cgtctatctg aacaaggacg
      121 gcagcgtgag gtcgcaggaa ccgtttctca ccggcttcat ccagaacaat gcctacatcg
      181 gccggcccgt ggacgtgcag gtcatgaagg acggctcgct gctcgtctcg gacgactacg
      241 ccggtgcggt ttaccgcgtg acttacggcg gcgacagggt cgcaaaacgc tgagcaggca
      301 ggagtccgcc ggaccgcggc ccggcagact tctttagcgg cccggcggaa tgatatgtcc
      361 gacaggctgc tgatgcgcag cgtccttgcc gccgccctcg ttctgttgcc ggcattgccg
      421 gccggcgcgg actcgctcga ggcgcggctc gcgcagtgtc tcgcctgcca cggcgagagc
      481 ggtcgatcgg cgacccccgg ggtgccctcg ctcggtggtc agccggcttt ctacatcacc
      541 atccaactat tcatgttccg ggagaagctg cgcgtcgtgg agccgatgag tgcgatgatg
      601 caagggatca gcaacgacga tttgcgcagg atggcggatt tcgtcgcaag gctgccgccg
      661 ccgcagccgg ccggcggttc ctccgatccc gagcgcctgg aacgggcgcg ggcgttgatc
      721 gagcagcacc gctgcaactt ctgccacaac cgggattatt ccggcgatca gaacgttccc
      781 cgccttgccg gccagcgcga ggactacctg gtgaaggcgc tgcgggaata caagagcaac
      841 gcgcgccgcg gctacgacgc ctcgatggcg gacgtcctca acgcaatcag cgacgagcag
      901 atcctcgacc tggcctactt cctggcccgt tttcgctgat agaatccgaa gagactaagc
      961 gcggacgtcc tggaggaatt ccatgcgtaa tgttcatttt tgggcgctgg tggttgcgct
     1021 tgccacttcg tccgcgctgg cgcagccgta tccctccaag ccgatccgcc tgattctccc
     1081 gtatccgccg ggcgggggtt ccgacacgat cggccgtccg ctcgcgcaga agatgtccga
     1141 aggcctcggg cagcaggtcg tggtggaaaa ccgcggcggc gccggcggca atatcggaat
     1201 ggaactcgtg gcgcgcgcgg cgccggacgg ctatacgctc gtgttcgcct tgagcccaca
     1261 gctcgccgtc aacgtcagtt tgtaccggaa gattccctac gatccggtca aggactacca
     1321 gccgatcacg ctgctcggct ccggcggcta catcctcgcc gtccacccgt cggtgccggc
     1381 aaaatcggtc aaggagctga tcgcgctcgc ccgctcgcgg ccgggggagc tgacctatgc
     1441 ctcgtcgggc aacggcagcg gcgcgcacct ggctaccgag ctcctcaaga ccatgaccgg
     1501 catcaaactg atccacgtgc cgtacaaggg tggcgggccg gcgctcgtgg atctgcttgc
     1561 gggacaggtg cagatactgt tcgcgaccca gctcgcgtcg gcccagcaca tccagagcgg
     1621 gcgcgtccgc gcgctcgccg tgagcaccgc caagcgctcg atgggaatgc ccgatctgcc
     1681 gaccgtagcc gaggcgggcg tgccgggcta cgactcgggc gtgtggtacg gcgtgctctc
     1741 gcccgcaggc acgccgcggg acatcatcat gaagctgaac ggcgaaatcg tgcgggtgct
     1801 gaaccagccc gattaccgca gcctgctcgt gaacaacacc atcgagccga tcggcggcac
     1861 gcccgagcag ttcggagcgt tcatccgctc cgaaatcaag aagtacgcga aagtcgtcaa
     1921 agacgccggc atgcgcatcg actgaccggg gaacggaggg caccatcgaa cgcaaggatt
     1981 tgggcatcgc cgtcgtcggc gccggccgca tcggcgggct gcgcgcgcgg ctcgcggcgg
     2041 agcatcccgc cgtcagattc atggcggttt ccgacgtcga gccggggcgg gcacgcgatc
     2101 tggcgcggaa gaccggcgcg cagttccaca cagccgacaa tgccgaggcc atcgctcggc
     2161 cggaagtcaa cgccgtcatc atctcctcca gcgagggggc gcacgtcgag ccgctcatcc
     2221 aggcgatcgg gctcggcaag ccggtgctgg tggaaaagcc gattgcgctc accctcgccg
     2281 atgccgaccg cgtcatccgc accgccgaga aggcgggcgt caacgtgcgc gtcggctaca
     2341 gccgccgcta caaggagcgt tacttgatcg ccaaggagca gatcgtgcag ggccgcctgg
     2401 gcagggtcgt cggcggcgcc gcgcgcgtat tcaacctgcg ctcgcaggca acggccatcc
     2461 ttcagcgcaa cccgcatgcg acgccggtcg tggacgcgct gacctattac gtggacctca
     2521 tgaactggat gcttgagggc acgcaggtga gggaagtctt cgcgcggggg cagaagggcg
     2581 tgctgaagga cgcgggcttc gacaccgagg acgtcagcta cgcgatcctc acctacgctg
     2641 acggcgcggt ggtgaacctc ggcatcgctt actcgctgcc ggaccagtac ccggcgcgcg
     2701 gccacgcggc gcgggtcgag gtgctcggca ccggtggcgt gatgatcctc gacgacgatc
     2761 acaccgatca attgatgtac tccgagaagg gcgtgccgca cgtgtatctc cccgaccagc
     2821 cggtgaacat ggtgttcctg cagagcggca cgccgggcga ctgggcgctg ggcgacttct
     2881 gggggccggt cgcgaacgag acgcgcgcct ggctcgacca cctcgcggtc ggcaagccgt
     2941 gcgcgctcgc cacggcacgc gaggcgcgcg ccacgctcga agtgacgctc gcgatcgagc
     3001 actcggcagc aagcggaagg cccgtcagcc ttccgctcgc tgaatgaaac tgaaaattgg
     3061 agcattgaca atgggtactt tcaggtttcg ctgggcactg gcggttgcag caatagtggt
     3121 catcccgaac gcggcggttg cgcaaagtta tccagcgaaa cctgtgcgga tgctcgtgcc
     3181 gcacgccccc ggcggtccga ccgacatcat ggcgcgcgtc gtcggccagc ggctctccga
     3241 gctgtggggc cagacgatcg tggtcgacaa ccgcgcgggc gcgggcggca acatcgggac
     3301 ggccctggtg gcgcgcgcca atcccgacgg ctacacggtg ctcgtcaaca gcagcgcgtt
     3361 cgccgtgaac gtcagcctgt tccgcaagcc gggctacgac ccgaaggagt tcgagccgat
     3421 catcaacggc ggcgtcatcc cgaacctgct gttcgcgcac ccgtcgtttc ccgccgccac
     3481 gctgcaggaa ctgatcaaga tcggcaaaac gaaaaaactg tcgtacgcat ccgccggcat
     3541 gggcacgacg ccgcacctca ccgccgagcg gttgttgaag acgatggcga agctcgacat
     3601 cacgcacgtt ccctacaacg gcgccgggct ggctgccaac gcggtggtgg ccaacgaggt
     3661 gccgatcggc tccgccgccg tgaccgcgcc ggcgccgctg atccgggccg gaaggctgaa
     3721 agggatcgcc gtgaccagcc tgcagcgcac gccggcgctg cccgatatcg cgacggtggc
     3781 ggagtcgggg ttcccgggat acgaggacta cacctggatc gcgttcttcg cgccgcgcgg
     3841 cacgccgcgc gcggtggtgg cgaaactcaa cgccgaaatc gcctcggcgc tccaggtgca
     3901 ggcgatcaag gagcggctcg cgaccctcgg cttcgatttc accccgaaca cgcccgagca
     3961 gttcggcgag tatctcaagc gcgaagtcgt gaagtggggg caggtggtga aagagtccgg
     4021 ggctagggtg gactgaagag cagggatgag ggatgagggc ggagggatga ggagttgagg
     4081 caggagaatc gaatcacgcc ttcctgccag gacttatact gtttggcgct tgtcgttccc
     4141 acctgaaatg aagggaggat tccatgcgga aaaaggcgag aaagaaaacc ggtcggaagc
     4201 tcaatccgga cgcttcgaaa actcagggca aggccaagaa cgaggatctg aagcaggatc
     4261 aggacgatat tcggagagcc gttgacgacg gaatgcagga tttgaggata a
//
LOCUS       DBNGWZ010000541         5814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2718492, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000541 DBNGWZ010000000
VERSION     DBNGWZ010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2718492"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..429)
                     /locus_tag="AC16CX_16830"
     CDS             complement(28..429)
                     /locus_tag="AC16CX_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014209.1"
                     /translation="MAGITVAQTPVSSWVEKTVHGYRIALAVESVPGTAPSKPDPKHA
                     SVLDHRLIVSFGDEKTGATADVSDASVDVAELGYKGVTVPLQRVESGKQVFYEARLRL
                     STKSPHRILVHATPTRGGRTLEAQFEYQHHH"
     gene            complement(588..1250)
                     /locus_tag="AC16CX_16835"
     CDS             complement(588..1250)
                     /locus_tag="AC16CX_16835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3014210.1"
                     /translation="MNHESGPAYGLWSLVIINSLVFIIFAFSFAKPQSSRDWRSFGAF
                     SAFLVALFTEMYGFPLTIYLLSGWLTSKFPEIDFFAHDSGHLLEVMFGWRSNPHFGVF
                     HILSNVLIFGSFILLASAWKVLYEAQRTHRLATTGPYAHVRHPQYSAFILIMFGFLLQ
                     WPTLITLVMFPILVYMYVRLAHREEREVAAEFGEEYLRYSKRTPAFFPRLDALFTRRG
                     DA"
     gene            complement(1348..2271)
                     /locus_tag="AC16CX_16840"
     CDS             complement(1348..2271)
                     /locus_tag="AC16CX_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR3014211.1"
                     /translation="MKRSLARGGMATFLASGVTLAVFAITPLLAPASAPPPRTAAEEK
                     PRFASLQVEIWPEFDRRGAALVILKGELGADLALPATVSLRVPASSDPTAVAFATAPG
                     SELFNLEHERIYADAFTTLRFTTSHRLVHVEFYDRLAPDLPDRRFTYVWPGDLAVDRL
                     TVRLQEPAAASDIVVKPELGAADKGPDGLLYRKAELGAFDAGKRLSIEIGYTKSDPRT
                     SSEILGLKAADSTPAPTTGSRIALPGWLLIVAIAAALLTGAGTVFLWWRLRRKASGAP
                     LRGAAFCPECGNRLASDGQYCSKCGAPVRKR"
     gene            complement(2268..2507)
                     /locus_tag="AC16CX_16845"
     CDS             complement(2268..2507)
                     /locus_tag="AC16CX_16845"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3014212.1"
                     /translation="MMWGDYGWGWGMGFGMISVVLFWVLVILGIVILVKWIAGGSSGP
                     GQPPGKTALDILKERYARGEIDREEFEQKKRDLAS"
     gene            complement(2530..2904)
                     /locus_tag="AC16CX_16850"
     CDS             complement(2530..2904)
                     /locus_tag="AC16CX_16850"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3014213.1"
                     /translation="MCHLILILPLLALPVFWLLPPGAAIPLYGAALAVTAGVYVLAVK
                     AMRKPVCTGKEDLLHEVGTVRAVRGDTLTVWVKSELWSAEDGDEAVAVGDAVEVLAVD
                     GLMLRVRKTRNAGATSETPGIH"
     gene            complement(2912..3571)
                     /locus_tag="AC16CX_16855"
     CDS             complement(2912..3571)
                     /locus_tag="AC16CX_16855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3014214.1"
                     /translation="MSEPEPAYGLWTLVIINSAVFIIFAFSFATPRTKRDWRSFGAFA
                     AFLVALFTEMYGFPLTIYLLSGWLTAKFPGVDFLAHDSGHLLEVFFGWQGDPHFGPFH
                     VASYVLIFAGFVLLAKSWGILYEAQRRRELATSGPYAWVRHPQYLGFILIMLGFLLQW
                     PTLLTLVMFPVLLYMYVRLARREEREVAAEFGEEYASYARRTPAFLPRLRALFALSHS
                     A"
     gene            complement(3606..3851)
                     /locus_tag="AC16CX_16860"
     CDS             complement(3606..3851)
                     /locus_tag="AC16CX_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HIR3014215.1"
                     /translation="MSPEQESHRKNGWRSKANWALVGFLLVAAYFLITEHRAHVIDFL
                     PFILVLACPLLHLFMHRDHGHGANRDHEHPGEGDKRE"
     gene            complement(3861..4598)
                     /locus_tag="AC16CX_16865"
     CDS             complement(3861..4598)
                     /locus_tag="AC16CX_16865"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR3014216.1"
                     /translation="MSDLSSLGVLTAFAAGIVSFLSPCVLPLVPGYISYVAGGSLQHP
                     RHLSVSQRLSAFAMSGLFVSGFAAVFLAFGASASALGQLLIKYRYEANLIGGAIVIGF
                     GLLMLGTPRWMPWLQRDVRFHPHVLGGHPVSAFVLGLAFGFGWTPCIGPVLGAILTVS
                     AASTTASGVGLLGAYALGLGVPFLASALFLDRAARLLREARRLGQLLQIAGGLLLVAL
                     GVAMITGWLTAFSFWLIETFPAFRTVG"
     gene            complement(4605..5360)
                     /locus_tag="AC16CX_16870"
     CDS             complement(4605..5360)
                     /locus_tag="AC16CX_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014217.1"
                     /translation="MANAVPAGGHPSRVVLADSIVKLVQEGVLDPQKIEALYARRGGI
                     PAGLKEILSKPSHRPILLTRETAYVHVNLLWPLGLANRMAANSESPLNGPKLFRFAST
                     GGWSLGKEANGGVYFNKFPIVELTVAQEALVTRVARNTFRPCCDNSTFYQDCNHGSAL
                     LGLLALGAAQGLNEEDLYREALAFNAFWFPHHYAHIALYFKVARNTEWRDVDPREVMS
                     ANFSSARGWNANVARELKRRGLLPEQGGADCGA"
     gene            complement(5495..>5814)
                     /locus_tag="AC16CX_16875"
     CDS             complement(5495..>5814)
                     /locus_tag="AC16CX_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3014218.1"
                     /translation="WCVECRAEHPLWMQIKAEGSVPLHGLNYKDRPDDAARWLGELGD
                     PYTRTGADLDGRVGIDWGVYGVPETFVVDKRGVIRHKVIGAVTRKTLDEKVLPLVRRL
                     QAE"
BASE COUNT         1161 a         1846 c         1834 g          973 t
ORIGIN      
        1 tcgtttcacc gccgatcgag ctgctcatca gtggtggtgc tggtactcga actgcgcttc
       61 cagtgtccgc ccgccgcgcg tcggtgttgc atggaccagt atccggtgcg gggacttcgt
      121 ggacaggcgc agccgggctt cgtagaagac ctgcttgccg gattcgaccc gctgcagcgg
      181 cactgtgacg cccttgtacc cgagttctgc gacatccacc gaagcgtcgg agacgtccgc
      241 ggtggcgcct gttttttcgt ccccgaacga cacgatcagg cgatgatcga ggactgacgc
      301 gtgcttcgga tccggcttcg aaggcgcggt gccgggcacg ctctcgactg ccagcgcaat
      361 gcggtatccg tgaaccgtct tctcgaccca ggaggaaacg ggggtctgcg cgacggtaat
      421 tccggccaac gccgcaagcc acatcccagc aaggcagcag cgccaagcgg tacgaagccg
      481 acccgccgcc cgcgatgcgg tcgagtccag gggcgcgacg cgtccgctgt caggattttg
      541 cgtttgtgtt gtcgtaacgc attcctcgcg ttcgccgcgc gatggtgtca cgcatccccc
      601 ctccgcgtga acagagcgtc caagcgcgga aaaaacgcgg gcgttcgttt tgaataacgc
      661 aagtactctt cgccgaactc agccgccacc tcgcgttcct cccgatgcgc caacctcacg
      721 tacatgtaaa ccaggatggg aaacatcacg agtgtgatga gcgtcggcca ttgcaggagg
      781 aagccgaaca tgatcaggat gaacgccgaa tactgcggat gccttacgtg tgcgtacggc
      841 ccggtcgtcg caagcctgtg cgtgcgctgc gcttcgtata gcactttcca cgcgctcgca
      901 aggagaatga agctgccgaa gatcagcacg ttgctcagga tgtgaaacac gccaaaatga
      961 ggattcgagc gccaaccgaa catcacttcg agcagatgtc ccgaatcatg ggcaaagaaa
     1021 tcgatttccg ggaacttgga agtgagccac ccagagagca agtagatcgt gagcggaaag
     1081 ccgtacatct cggtgaacag cgcgaccagg aacgcggaga atgcgccgaa cgagcgccag
     1141 tcacgcgaac tctgcggctt ggcgaagctg aacgcgaaga taatgaacac cagcgagttg
     1201 atgatcacga gcgaccacag gccgtaagcg ggtccagatt cgtggttcat tcactcattc
     1261 cttttgagcg atcttcgtga tgatgtttac gctgtgtgca gctccttggt cgggaagacc
     1321 gccgtgcatg aaaaagcggt tctgttgcta gcgcttgcgc acaggcgcgc cgcacttcga
     1381 gcagtattgg ccgtccgagg cgagccggtt gccgcactcc ggacagaagg ctgccccgcg
     1441 caatggtgcg ccggacgcct tcctgcgcag gcgccaccac agaaacaccg tcccggctcc
     1501 agtgaggaga gccgccgcga tcgcgacgat caggagccag ccgggcagtg caatacggga
     1561 acccgttgtc ggcgcgggtg ttgaatccgc cgctttcagc ccgagtatct cggaggaggt
     1621 tcgcgggtcc gacttcgtgt aaccgatctc aatggacagc cgctttcccg catcgaaggc
     1681 gcccaactcg gccttccggt agagcagccc gtccgggcct ttgtccgcgg cgccgagctc
     1741 gggcttcacc acgatgtcgc tcgctgccgc aggctcctgc agacgtacgg tcaatcggtc
     1801 tacggccaag tcgcccggcc atacatacgt gaagcggcga tcgggcaaat ccggcgcaag
     1861 ccggtcgtag aactcgacgt ggacaaggcg gtgcgaagtc gtgaatcgca gcgtggtgaa
     1921 cgcgtcggca tatatccgct cgtgctccag attaaaaagc tccgagcccg gtgcggtggc
     1981 gaacgctacc gccgtcgggt cgctggaggc agggacgcga agggataccg tggcgggaag
     2041 ggcaaggtcg gcgccgagct cgcccttgag gatcacgagc gccgctccgc gccggtcgaa
     2101 ctccggccag atttcgacct gaaggctcgc gaagcgtggc ttctcttcgg ccgcagtgcg
     2161 ggggggtggg gcggaagcgg gcgcgagcag gggcgtgatg gcgaagacgg ccagcgtcac
     2221 tcctgacgcg aggaacgttg ccatgccacc ccgggcgagt gaacgtttca tgacgcgaga
     2281 tcgcgcttct tctgctcgaa ctcctcgcgg tctatttcgc cgcgggcgta gcgctccttc
     2341 agaatgtcca gcgcggtctt cccgggcggc tggcccggcc cggaggaacc gcccgcgatc
     2401 catttcacca ggatcacgat gccgagaatg accagcaccc agaacagcac cacgctgatc
     2461 atcccgaatc ccatacccca tccccatcca tagtcgcccc acatcatgtg cgttctcctt
     2521 tcgaatcaat tagtggatac ccggcgtctc cgaagtcgca ccggcatttc gggtcttgcg
     2581 tacccgaagc atcaggccat cgaccgccag cacctccaca gcatcaccca cggccacggc
     2641 ctcatcgccg tcttcggcgg accagagctc gctcttgacc cacaccgtaa gcgtgtcacc
     2701 gcgcaccgcg cgcaccgtcc cgacctcgtg cagcaggtct tccttgcccg tgcaaacggg
     2761 cttgcgcatc gcttttaccg cgagcacgta gacgccggcg gttacggcga gcgccgctcc
     2821 gtaaagcggg attgccgcgc ccgggggcaa cagccagaaa accggcagcg cgagcagtgg
     2881 aaggatgagg atcaggtggc acatagccgt attacgcgct gtggctcaga gcgaacaatg
     2941 ccctcagccg cggcaaaaac gcgggggtcc gcctcgcgta gcttgcgtat tcctctccga
     3001 actcggcggc cacttcgcgt tcctcgcggc gcgcgagccg cacgtacatg tagagaagaa
     3061 cggggaacat gaccagcgtc agcagcgtcg gccattgcag gaggaagccc agcatgatga
     3121 gaatgaaacc gaggtactgg gggtgacgta cccacgcata aggtccactc gtcgcaagct
     3181 cgcggcgccg ctgcgcctcg tacaggattc cccaggactt ggcgagcagg acgaagcccg
     3241 cgaagatcag aacgtaggag gcgacatgaa acggaccgaa atgcggatcg ccttgccagc
     3301 cgaaaaagac ttcgaggagg tgcccggaat cgtgcgcgag aaagtccacg ccgggaaact
     3361 tggccgtgag ccatcccgag agcaggtaga tcgtcagtgg aaatccgtac atctcggtga
     3421 acagcgcgac cagaaatgca gcgaatgcgc cgaacgatcg ccaatcccgc ttggtccgcg
     3481 gcgtggcgaa gctgaacgca aagatgatga agacggcgga attgatgatt accagcgtcc
     3541 acaggccgta ggctggttcg ggctcgctga tggcgagcgt ccacagggtg tcggccgact
     3601 cgtgctcact cacgtttgtc tccttctccg ggatgttcgt ggtcacgatt tgcgccgtgc
     3661 ccatggtcgc ggtgcatgaa caggtgcagc agcgggcagg cgagcaccag gataaaagga
     3721 agaaaatcga tcacgtgcgc gcggtgctcg gtgatcagga aatacgccgc caccaggaga
     3781 aagccgacga gcgcccaatt ggctttcgac cgccagccat tcttgcgatg ggattcttgt
     3841 tcgggcgaca tacagcggtc tcaacccacc gtgcgaaacg cagggaaggt ctcgatcagc
     3901 caaaatgaga acgcggtcag ccagccggtg atcatggcaa cgccgagcgc gacgagcagc
     3961 agcccgccgg cgatctgcag caattgcccc agcctgcgcg cttcccgcag cagccgcgcc
     4021 gcgcgatcca gaaacagcgc cgatgcgagg aacggcacgc cgagcccgag cgcgtaggcg
     4081 ccgagcaatc cgacaccgct cgcggtagtg ctcgcggcgc tgacggtcag gattgcgccg
     4141 agcaccggtc cgatgcacgg ggtccagccg aagccgaacg ccagtccaag cacgaacgcg
     4201 gacacgggat gaccgccgag aacgtgcggg tgaaacctca cgtcgcgctg cagccacggc
     4261 atccagcgcg gcgtgccgag catcagcagc ccgaagccga tgacgatcgc gccgccgatg
     4321 agattcgcct cgtagcggta cttgatgagc agctgcccga gcgcgctcgc gctcgccccg
     4381 aaagcgagaa agaccgccgc gaacccgctc acgaacaacc cgctcatcgc gaatgccgaa
     4441 aggcgctggg agacgctcag gtgccggggg tgctggagac tcccgccggc gacatacgag
     4501 atgtagcccg gcacgagcgg caggacgcag ggcgagagaa acgagacgat ccccgccgca
     4561 aaagcggtca acacgccgag gctggacaga tcgctcatcg aacgtcaggc gccgcagtct
     4621 gcgccgcctt gctcgggcag caggccgcgc cgcttcaact ctctggcgac attcgcgttc
     4681 cagccgcgtg cgctggagaa attcgcgctc atgacttccc gcggatcgac atcccgccat
     4741 tccgtgttcc ttgccacctt gaagtagagc gcgatatggg cgtaatggtg gggaaaccag
     4801 aaggcgttga aggcgagtgc ttcgcggtag agatcctctt cgttcaatcc ttgcgctgcg
     4861 ccgagcgcaa gcagcccgag aagcgcggag ccatggttac agtcctgata gaacgtcgag
     4921 ttgtcgcagc aggggcggaa agtgttgcgg gccacccggg tcaccagtgc ctcctgtgcg
     4981 acggtgagct cgacgatggg gaacttgttg aagtagacac cgccgtttgc ttccttgccc
     5041 aggctccatc cgcctgtcga ggcgaagcga aaaagcttgg gtccgttgag ggggctttcg
     5101 ctgttcgccg ccatgcgatt cgcgagcccc agcggccaca ggagattcac gtggacgtat
     5161 gcggtttccc gggtgagcag gatgggccgg tgcgaaggct tgctcaggat ttccttcagt
     5221 cctgcgggga taccgccgcg ccgcgcgtaa agggcctcga tcttttgcgg gtcaaggacg
     5281 ccttcctgca cgagcttgac gatgctgtct gcgagcacca ccctgctggg gtgcccgcca
     5341 gctggcaccg catttgccat ggcctccttg aacaattgac tccttgcgga gacgggcgcc
     5401 gcaatcgcca gcacggggac cgtcagaaag gccagagcac acgatctgaa tgtagttgtc
     5461 atctgagcag cctcgcttga tcgaggcgca agcctcattc cgcctgcagc ctgcgcacca
     5521 ggggcagcac cttttcgtcg agcgtcttcc tcgtgaccgc gccgattacc ttgtgccgga
     5581 tcacgccgcg cttgtcgaca acgaacgtct cgggaacgcc gtagacgccc cagtcgattc
     5641 cgacccggcc gtcgaggtcc gcgccggtgc gcgtataggg atcgccaagc tcgccaagcc
     5701 accgtgcggc gtcatccggc cggtccttgt aattgagacc gtgcagcggg acgctgccct
     5761 ccgccttgat ctgcatccac agcggatgct ccgcgcgaca ctccacgcac cacg
//
LOCUS       DBNGWZ010000542        12146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1337025, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000542 DBNGWZ010000000
VERSION     DBNGWZ010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1337025"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /gene="rnpB"
                     /locus_tag="AC16CX_16880"
     ncRNA           <1..165
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16CX_16880"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            492..938
                     /gene="mraZ"
                     /locus_tag="AC16CX_16885"
     CDS             492..938
                     /gene="mraZ"
                     /locus_tag="AC16CX_16885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR3014219.1"
                     /translation="MFRGVAQLNLDSKGRLAVPSKFRDDLISRSSGRLVITADSDRCL
                     LIYPLPDWEPIEQRLMALSSFNAQIRELQRRLVGYAEDVVMDATGRILVPPALRRFAQ
                     LEKAVMLIGQGNKFELWNKDNWEQRMEQGGGFSPGGLPPELEGFSL"
     gene            935..1864
                     /gene="rsmH"
                     /locus_tag="AC16CX_16890"
     CDS             935..1864
                     /gene="rsmH"
                     /locus_tag="AC16CX_16890"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR3014220.1"
                     /translation="MSGGSHTAVLLEQAVDGLKVRGDGCYVDCTFGRGGHSRLILARL
                     GRAGRLIALDRDPQAVAAGGEIDDRRFRILHGRFGRLAELAARASVTRADGILLDLGV
                     SSPQLDDPARGFSFRFDAPLDLRMDPGEGITAAEWLAHAPEQEIREVIKNHGEERFAK
                     QIAAAIVAARARGPVGTTRQLATLVAKAVPTREPQQDPATRTFQALRIHINQELEELS
                     LALPQCVELLKPGGRLVVISFHSLEDRIVKRFLRDNARPDRLPPRLPLRARELPRPRL
                     KLIGRAARPSAVEITANPRARSAVMRVAEKLGA"
     gene            1865..2146
                     /gene="ftsL"
                     /locus_tag="AC16CX_16895"
     CDS             1865..2146
                     /gene="ftsL"
                     /locus_tag="AC16CX_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR3014221.1"
                     /translation="MVRVNLLLLAALTACALALVTSQHQARKLYVELQKEQDLARQLE
                     VEWGQLQLEASTWATHARVEKIAVQSLGMRVPTASRIQVAPPVAGEGRQ"
     gene            2143..3876
                     /locus_tag="AC16CX_16900"
     CDS             2143..3876
                     /locus_tag="AC16CX_16900"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3014222.1"
                     /translation="MNAALHPQLAVKLPAWRARVLLGLLLAAFAALAGRAFFLQGLHN
                     DFLQQKGESRYSRVIELSATRGRIVDRNNNPLAISTPVESVAASPVDVDVSAAQLKRL
                     ARLLEVDAGELARKLSDSKREFVYLKRQLPPEEAARVVGLGIPGVFLQREYRRYYPAG
                     EVTAHLIGFTDVDGRGQEALELAFEQSLAGQPGSRRVIKDRLGHIVEDMESIRTPQDG
                     ATLRLSIDAKIQYLAYRELKAAVAAHRARSGGVVVLDVASGEVLAMVNVPSYNPNNRG
                     KLDPRRTRNRAVTDLFEPGSTLKPFTAAAALEAGTVSPESVIQTAPGSLAIGNRLIHD
                     AHPQGALTVAQVIQKSSNIGAAKMALGLKPETLWNLFNHVGFGVPPRSGFPGEVSGKL
                     RAYASWRPIEQATMSYGHGISVSLLQLARAYLIFATDGELRPVTLVRREASAETTQVV
                     SPATAQAVRRMLEMAVQPGGTAPRAQVTGYRVAGKTGTAHKLEGRGYAPEKYVSTFVG
                     FAPASSPRLVVAVMIDEPSAGQYYGGAVAAPVFSQVMAGALRLLATAPDAPVDNVVLP
                     PPDAQEIKEEV"
     gene            3876..5405
                     /locus_tag="AC16CX_16905"
     CDS             3876..5405
                     /locus_tag="AC16CX_16905"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3014223.1"
                     /translation="MPRFGMRDTGFGKGALTRADRAAIEALGVRRLVNDSRAVRPGDT
                     FVAYPGQARDGRDYIAQAIANGAASVLWERAGFEWNAQWRAPNLGVRDLRRHAGEIAS
                     RLHGRPSGRLRMIGVTGTNGKTTCSQWIAQALTRAGHRCAVIGTLGYGLGTRLKPIVN
                     TTPDALWLHAQLAEFARRGAQAVAMEVSSIGLDQDRVAGIEFDVALFTNLSRDHLEYH
                     RTMRRYREAKARLFAWKTLKHAVVNLDDDFGVELARRIRRPGLNVIGYGFGAARGTRA
                     ERVAGANLVTGAHGVAFDVRTPWGNGRVTSPVLGRYNACNLLGTLSVLLASGVALRKA
                     IAVLGALKPAPGRMQRLGGGSKPLVVVDYAHTPDALKQVLTTLRELLGDPSRVTSHES
                     RLICVFGCGGDRDRGKRPLMGGVAAKLAERVIVTSDNPRGEDPRGIIEDIVAGVHGAA
                     NEIVIEADRRAAIRRALAEARRGDIVLVAGKGHEEYQEIRGTRHPFSDAAVAREALEN
                     R"
     gene            5402..6832
                     /locus_tag="AC16CX_16910"
     CDS             5402..6832
                     /locus_tag="AC16CX_16910"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3014224.1"
                     /translation="MSMMRLSEAARAIPAELRGEDRVFESVSTDTRALSPQALFVALK
                     GGRYDGHDFLAQAAEQQAAGALVQRSGLGTRNPGLDLPLLVVDDSRLALGTLAAYWRN
                     KFSMPLVALTGSNGKTTVKEMLASILREAVLDETRDPSPESRVHATRGNLNNDIGVPL
                     TLLEIRTRHRYCVVEMGMNHAGEIRYLARLAAPDVALINNAGPAHIEFFASVEAVARA
                     KGEIFEGLKPGGTAVINADDRHAGLWRELAHGRRVVEFGLSHPAAVSARYALRYLESE
                     IVVRTPLGEAQAVLKAPGLHNVRNALAASAAAVALQVPAPAIARGLERFTGIKGRLQK
                     KAGLNGATLIDDTYNANPDSVQAAIAVLAQAPGEKLLVLGDMGELGPGAPELHTEVGR
                     FAREQGIGRLLTLGELSAHAARAFGAGARHFTRVEDLLAEAENALAPGVTALIKGSRF
                     MQMERVVKAFAVGGEERGERREEARP"
     gene            6891..7976
                     /gene="mraY"
                     /locus_tag="AC16CX_16915"
     CDS             6891..7976
                     /gene="mraY"
                     /locus_tag="AC16CX_16915"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3014225.1"
                     /translation="MLLELAQWLAKDIRAFNVFNYITLRAVLACLTALVISFIIGPAL
                     IRKLTTYKVGQAVRDDGPQTHLAKAGTPTMGGALILASITITVLLWADLQNRLVWIVL
                     WVTLGFGAIGWVDDYRKVVARNPRGLSARAKLFWQSVIGVAAACYLAFYTSLPAQTEF
                     IVPFFKQVAYPLGALGFIVMTYFVIVGTSNAVNLTDGLDGLAIMPTVMIGGALGIFAY
                     VAGHAVFAKYLGFPYIPGAGELAVVCGAIAGAGLAFLWFNAYPAEVFMGDVGALALGA
                     ALGTIAVIVRQEIVLFIMGGVFVVETLSVMLQVASFKLTGKRIFRMAPLHHHYELKGW
                     KENQVVVRFWIITMMLVLVGLSTLKLR"
     gene            8083..9462
                     /gene="murD"
                     /locus_tag="AC16CX_16920"
     CDS             8083..9462
                     /gene="murD"
                     /locus_tag="AC16CX_16920"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR3014226.1"
                     /translation="MLDLNGRRVVVLGLGDTGLSMARWLARRGADVRVADSRKAPPQA
                     PALARALPQVRLETGAFSDDTMKDADLIAISPGIDRREAPIASAIERGVPVVGDVELF
                     AQALKTLIPPKVLAVTGSNGKSTVTAMAGDMCRAAGRETVVAGNIGVPVLDALTAIEE
                     RGTAPEVFVLELSSFQLESTASLQPDAATVLNVTEDHLDRYDGMADYAAAKARVFSGN
                     GVQILNRDDRSTLNMARAGRTLFTFGFSAPRGESEWGITPEGGARALAHGGRRLMAVA
                     ELPLAGLHNAANALAAHALAHAIGLPDGPLIDALRRFRGLPHRMRKVAEIAGVAFYDD
                     SKGTNVGATVAALSGLAQPVVLIAGGDGKGQDFSPLAPAVKARARAVVLIGRDAALIE
                     RAIAGSGARIERAAGMDEAVDVAARAAQPGDAVLLSPACASYDMFRDYVHRAEVFAAA
                     VRRLEDRRR"
     gene            9466..10626
                     /gene="ftsW"
                     /locus_tag="AC16CX_16925"
     CDS             9466..10626
                     /gene="ftsW"
                     /locus_tag="AC16CX_16925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR3014227.1"
                     /translation="MFYTDVKPRAPIAEYDQSLVWTAVLLIGLGLVMVYSASIAIAEG
                     SRATGNQPTYYLMRHGLFVAVGIVASVIAFQIPLRLWQQAAPFLFLFGTGLLILVLVP
                     GVGREVNGSQRWLSLHFVTLQPSEFVKLAAVLYAADYTVRKAAHMASFRKGFLPMFVV
                     MLVVGGLLLREPDFGAFVVISVLALGILFLGGMNWKLFAGLFGLLLVGFVALILASPY
                     RLQRVSGFIDPWADPYGRGYQLSHALIAFGRGEWLGVGLGGSVEKLFYLPEAHTDFLL
                     AVIAEELGFAGMAAIILAFTWIIARAFMIGRRAASLERHFPALVAQGIGLWIGVQAII
                     NIGVNTGMLPTKGLTLPLVSFGGSALVSTCCALAVLLRVDWENRQLMRGFTV"
     gene            10643..11740
                     /gene="murG"
                     /locus_tag="AC16CX_16930"
     CDS             10643..11740
                     /gene="murG"
                     /locus_tag="AC16CX_16930"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR3014228.1"
                     /translation="MGDERKNPGAPRTIMIMAGGTGGHIFPALAVAEHLRALGWNVVW
                     VGVRGGMEERLIPPRGYTLSWIRFSGVRGRGIVAAALLPLNLLIAFWQTARSIFAHRP
                     DVVLGMGGYVSFPGGMMASFLNRPFAIHEQNSVAGLANRVLAKLADRVLTSFPNAFGE
                     ATAVIWTGNPVREEIAAMAPPENRYAARAGQLRLLVVGGSQGAQVLNAVVPEALALIA
                     PEVRPRVRHQAGAVHHDAVRARYQALGVAADVVAFIDDMAGRYAETDLIVCRAGATTV
                     AEIAAAGIASVLVPYPHSVDDHQTINARFLADRGAALLIPQRELTARRLAEVLSGFTR
                     ERLLAMAQSARAAGRPEATRVVAQACMELAA"
     gene            11948..>12146
                     /locus_tag="AC16CX_16935"
     CDS             11948..>12146
                     /locus_tag="AC16CX_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HIR3014229.1"
                     /translation="MRHRVKHVHFVGIGGAGMSGIAEVLLTLRYKVSGSDLVENASTR
                     RLRQLGAKIHIGHDEAHIAGAD"
BASE COUNT         1979 a         3914 c         4253 g         2000 t
ORIGIN      
        1 cgaggcaacc tccacccgga gcaacaccaa ataggcaagc gatgacgtga cccgccgagc
       61 ttgcgggtag gtggcttgag ccctggagca atccagggcc tagaggaatg gctgtcggag
      121 accgcgaggt cctcacagaa cccggcttac cggctggctt cccacttctt tcagttttat
      181 tcaatggctg ttgccacccc gcaacagctt tcaatttcca caatcctgta cgctgagcgc
      241 acaaacttaa agtattactt tcggtttact ttttatcacg ctgtttttaa gcactaatta
      301 gagttgaaat ccaagctaaa aacggcttca atttttcctc ataagtcatt gtccttaaaa
      361 cagaaaacgc ttgataaccc ttgaccgttt cccgctcttt ccaatatagt gggaagaagt
      421 ggagaaaagt gggataaacc gtcacctcga aaagaagggg gcggtgttgt cccgcgagcc
      481 aagggggact cgtgtttcgt ggcgtcgcgc aactgaatct cgacagcaag gggaggctgg
      541 cggtgccctc caagtttcgc gacgacctca tctcacgcag cagcggacgt ctcgtcatca
      601 ccgccgattc cgaccgctgc ctgctcatct atccccttcc cgactgggaa ccgatcgaac
      661 agaggctgat ggcgctgtcg agcttcaacg cgcagatacg cgaattgcag cggcgcctcg
      721 tcggctacgc cgaggacgtc gtgatggacg cgaccggacg catcctggtg ccgccggcgc
      781 tgcgccgctt tgcgcagctc gaaaaggccg tgatgctgat cggacagggc aacaagttcg
      841 agctgtggaa caaggacaac tgggaacagc ggatggagca gggcggcgga ttcagcccgg
      901 gcgggctgcc gcctgagctg gagggtttct ccttgtgagc ggtggctcac ataccgcggt
      961 cctgttggaa caagcagtag acggtttgaa ggtgcgcggc gacggctgct acgtcgactg
     1021 cacgttcggt cgcggcgggc acagtcggct gatcctcgcg cggctgggaa gggcagggcg
     1081 gctcatcgcg ctcgaccgcg acccgcaggc ggtggccgca ggaggagaga ttgatgacag
     1141 gcgttttcgc atcctgcacg ggcggttcgg tcggctcgcc gagcttgcgg cgcgagcgag
     1201 cgtcacccgg gcggacggca tcctgctcga tctcggcgtg tcatcgccgc agctcgacga
     1261 ccccgcccgc ggcttcagct tccgtttcga cgcgccgctc gacctgcgca tggatccggg
     1321 cgaaggcatc acggccgcgg aatggctcgc gcacgcgccc gagcaagaga tccgggaggt
     1381 cataaaaaac catggcgaag aacggtttgc taaacagatt gcagcagcga ttgttgcggc
     1441 tcgagcgcgg ggacctgttg gcaccacgcg acaacttgcc acgctcgtgg caaaggccgt
     1501 tcctacgcgc gagccacaac aggatccggc gacgcgcacg tttcaggctc tacggattca
     1561 catcaatcag gagcttgagg aattgtcgct agccctgccg caatgcgtgg aactcctcaa
     1621 gcccggggga cgtctggtgg tgatcagctt ccattcgctg gaggaccgca tcgtcaagcg
     1681 cttcctgcgc gataacgccc gtcccgaccg gctgccgccg cggctgccgc tgcgcgcgcg
     1741 cgagttgccc cggccgcggc tgaaactcat cggccgggcg gcgcggccgt ccgccgtcga
     1801 aatcacggcc aatccccgcg cgcggagcgc ggtgatgcgg gttgccgaga aactgggggc
     1861 atagatggtg agggtgaacc tcctgctgct cgccgccctg accgcctgcg cgctcgcgct
     1921 cgtcacctcc cagcaccagg cgcgcaagct ctacgtcgag ctgcagaagg agcaggatct
     1981 cgccaggcag ctcgaggtcg agtggggaca gctgcagctc gaggcgagca cctgggcgac
     2041 acacgcgcgc gtggagaaaa tagccgtcca gtcgctcggc atgcgggtgc caacggcttc
     2101 ccgcatccag gtggcgccgc ccgtggccgg ggaaggtaga cagtgaacgc tgcactccat
     2161 ccgcagctcg ctgtgaagct cccggcgtgg cgcgcgcgcg tgctgctcgg gctgctgctc
     2221 gccgcgttcg cagcgctcgc gggccgcgca ttcttcctgc aggggctgca caacgacttc
     2281 ctgcagcaga agggtgagtc gcgctactcg cgggtgatcg agctcagcgc cacgcgcggc
     2341 aggatcgtgg accgcaataa caatccgctt gcgatttcca cccccgtcga atcggtggcg
     2401 gcgagcccgg tggacgtcga tgtttccgcg gcgcagttga agcgtctcgc ccgtttgctc
     2461 gaggtggacg cgggagagct cgcgaggaag ctctccgatt cgaagcgcga gttcgtgtac
     2521 ctgaagcggc agctgccgcc ggaggaagcc gcgcgcgtcg tggggctcgg gattcccggc
     2581 gtgttcctgc agcgcgaata ccgccgctat tacccggccg gcgaagtcac ggcgcacctc
     2641 atcggtttta ccgacgtgga tggacgcggg caggaagcgc tcgaactcgc gttcgagcag
     2701 agcctcgcgg gccaaccggg cagccggcgc gtgatcaagg accggctcgg ccacatcgtg
     2761 gaggacatgg agagcatccg cacgccgcag gacggagcta cgctacggct ctcgatcgac
     2821 gccaagatcc agtacctcgc ctatcgcgag ctgaaggccg cagtggccgc gcaccgggcg
     2881 cggtccggcg gcgtcgtggt gctcgatgtg gcgagcggcg aagtgctggc gatggtgaat
     2941 gtgccttcct acaaccccaa caaccgcggc aagctcgatc cccggcgcac gcgcaaccgc
     3001 gcggtgaccg atctgttcga gccgggctcc acactcaagc ccttcaccgc cgccgcggcg
     3061 ctcgaagccg gcacggtgag ccccgagagc gtgatccaga ccgcgccggg gagcctcgcc
     3121 atcggcaacc gcctcatcca cgacgcgcat ccccaaggcg cgctcaccgt agcacaggtg
     3181 atccagaaat cctccaacat cggcgccgcc aagatggcgc tcgggttgaa acccgagacg
     3241 ctgtggaact tgttcaacca cgtcggcttc ggcgtgccgc cgcgctcggg ctttccgggc
     3301 gaggtgtcgg ggaagctgcg cgcctacgcg agctggcgcc ccatcgagca ggcgacgatg
     3361 tcctacggcc acggaatttc ggtatcgctg ctgcagctcg cgcgcgctta tctcatcttt
     3421 gccaccgacg gcgaattgag gccggtaaca ctggtccggc gcgaggcgtc cgccgaaacg
     3481 acccaggtcg tctcaccggc gaccgcccag gcagtgcgcc gcatgctcga gatggcggtg
     3541 caaccgggag gcaccgctcc gcgcgcgcag gtgaccggct accgcgttgc cggcaagacc
     3601 ggcaccgccc acaaactcga gggcaggggc tatgcgcccg aaaaatacgt gtccaccttc
     3661 gtcggcttcg cgcccgcgtc cagcccgcgg ctggtggtgg cggtgatgat cgacgaacct
     3721 tcggccggac agtactacgg cggcgcggtc gccgcgccgg tattcagcca ggtcatggcg
     3781 ggggcgctgc ggctgctggc gactgcgccg gatgcgccgg tggacaacgt ggtgctgccg
     3841 ccgccggacg cccaggagat caaggaagaa gtgtgatgcc gcgattcggg atgcgggata
     3901 cgggattcgg gaaaggcgcg ctgacgcggg cggaccgggc cgcgatcgag gcgctgggtg
     3961 tgcggcggct cgtgaacgac agccgagcgg tgagaccggg cgacaccttc gtcgcctatc
     4021 ccgggcaggc gcgcgacggc cgcgactaca tcgcgcaggc gatcgcgaac ggcgcggcga
     4081 gcgtgctgtg ggagcgcgcc gggttcgaat ggaatgcgca atggcgggcg cccaatctcg
     4141 gcgtgcggga tctgcgccgg cacgcgggcg agatcgcgag ccgcctccac ggccgtccaa
     4201 gcggccggct gcgcatgatc ggtgtgaccg gcaccaacgg caagaccacg tgcagccagt
     4261 ggatcgcgca ggcgctgacg cgcgctggcc atcgttgcgc ggtgatcggc acgctcggtt
     4321 acgggctggg cacgaggttg aagcccatcg tcaacaccac gccggacgcg ttgtggctgc
     4381 acgcgcagct cgcggaattc gcccgccgcg gcgcgcaggc ggtggcgatg gaggtgtcct
     4441 cgatcggact cgaccaggac cgcgtcgcgg gcatcgagtt cgatgtcgct ctcttcacca
     4501 atctgtcgcg cgaccatctc gagtaccacc gcacgatgcg gcgctaccgc gaggccaagg
     4561 cacggctgtt cgcctggaag acgctcaagc acgccgtggt gaatctcgac gatgatttcg
     4621 gcgtcgagct cgcgcgccgc atccgcagac ccgggctgaa cgtgatcgga tatggtttcg
     4681 gcgcggcgcg cggcacgcgg gccgagcgcg tcgcgggcgc taacctcgtc accggcgcgc
     4741 acggtgtcgc gttcgacgtc aggacgccgt ggggcaatgg gcgggtcacg agcccggtgc
     4801 tcggtcgtta taacgcctgc aacctgctcg gcacgctgtc cgtcctgctc gcgagtggcg
     4861 tcgcgttgcg caaggcgatt gccgtactgg gcgcgctcaa acccgcgccc ggccggatgc
     4921 agcgcctcgg cggcggctcg aaacccctcg tggtcgtgga ttacgcccac acccccgacg
     4981 ccctgaaaca ggttcttacg acactgcgtg agctgctagg cgacccgtca cgagtcacga
     5041 gtcacgagtc acgcctcatc tgcgtttttg gctgcggcgg cgaccgcgac cgcgggaagc
     5101 gcccgctgat gggaggcgtc gcggcaaaac tggccgagcg ggtgatcgtc acttcggaca
     5161 acccgcgcgg cgaggacccg cggggaatca tcgaggacat cgtcgcgggc gtgcacgggg
     5221 cggcgaatga aatcgtgatc gaagccgacc ggcgagccgc gatccggcgc gcgctggcgg
     5281 aagcccggcg cggcgacatc gtgcttgtgg cgggcaaggg gcacgaggag taccaggaaa
     5341 tacgcggaac ccggcatccg ttctcggacg ctgccgtggc gagggaggcg ctggaaaacc
     5401 gatgagcatg atgcgcctgt cggaagccgc gcgcgcgatc ccggcggaat tgcggggaga
     5461 ggatcgcgtc ttcgagagcg tcagcaccga cacccgcgcg ctctcgccgc aggcgttgtt
     5521 cgtcgcgctc aagggcggcc gctatgacgg ccacgatttt ctcgcgcagg cggcggaaca
     5581 gcaggcggcc ggggcgctcg tccaaagatc gggactcggg actcggaacc cgggactgga
     5641 ccttcccttg cttgtcgtcg atgactctcg gctcgcgctt ggaacgcttg cggcttactg
     5701 gaggaacaag ttctcgatgc cgctggtggc gctcactggt agcaacggta agaccacagt
     5761 gaaagaaatg ctcgcgtcga tcctgcgcga ggcggtcctg gacgagaccc gagacccgag
     5821 tcccgaatcc cgggttcatg ccacgcgcgg caacctgaac aacgacatcg gcgtgccgct
     5881 cacgctgctc gagatccgca ccagacaccg ttattgcgtg gtcgagatgg gcatgaacca
     5941 tgccggcgaa atccgttacc tcgcgcggct cgccgccccg gacgtcgcgc tcatcaacaa
     6001 cgccgggccg gcgcacatcg aattcttcgc ctcggtcgag gccgtcgcgc gtgccaaggg
     6061 cgagatcttc gagggcctga agcccggcgg cacggcggtg atcaacgccg acgaccgcca
     6121 tgctggcctg tggcgcgagc tggcccacgg ccgccgcgtc gtggagttcg gcctctctca
     6181 tccggcggcg gtgagcgcgc gctacgcgtt gcgctacctg gagagcgaga tcgtggtccg
     6241 cacaccgctc ggcgaagcgc aggccgtgct gaaagcgccg ggactgcaca acgtgcgcaa
     6301 cgcgctggcc gccagcgccg cggcggtggc gctgcaagtg ccggcgcccg ccatcgcgcg
     6361 cgggctcgag cgcttcaccg gcatcaaggg ccgactgcag aagaaggcgg ggctgaacgg
     6421 cgccacgctg atcgatgaca cctacaacgc caatcccgac tcagtgcagg cggcgatcgc
     6481 ggtgctggcg caggcgcccg gcgaaaagct gctggtgctg ggcgacatgg gcgagctcgg
     6541 tcccggcgcg cccgagctgc acacggaagt agggcggttc gcgcgggagc aaggcatcgg
     6601 gagattgctc acgctggggg agctgtcggc ccacgcggcg cgcgcgttcg gcgccggcgc
     6661 gcgccatttc acgcgtgtcg aggacctgct cgccgaggcc gagaacgcgc tcgcgcccgg
     6721 cgtcacggcg ctgatcaagg gttcgcgctt catgcagatg gaacgcgtcg tcaaggcatt
     6781 cgccgttggc ggagaggaaa gaggagagag gagagaggag gcaagaccgt gagcgggttt
     6841 ctcacctcac ccttaactcc tcacccctca ctcgcaaccg gaggttgtgg atgctgctag
     6901 agctggcgca atggctcgcc aaggacatcc gcgccttcaa cgttttcaac tacatcacgc
     6961 tgcgcgcggt gctggcgtgc ctcaccgcgc tcgtcatctc cttcatcatc gggccggcgt
     7021 tgatcaggaa gctcaccacc tacaaagtcg gccaggcggt gcgcgacgac ggcccgcaaa
     7081 cgcacctcgc caaagccggc acgccgacca tggggggtgc gctgatcctg gcgtcgatca
     7141 ccatcaccgt gctgctgtgg gcggacctgc agaaccgcct ggtctggatc gtgctgtggg
     7201 tgacgctcgg cttcggtgcg atcggctggg tggacgacta ccgcaaggtg gtcgcgcgca
     7261 accccagggg gctgtcggcc agggcgaaac ttttctggca gtcggtgatc ggcgtcgccg
     7321 cggcgtgcta cctcgcgttc tacaccagcc tgccggcgca gaccgagttc atcgtgccgt
     7381 tcttcaagca ggtcgcctac ccgctcggcg cgctcggctt catcgtcatg acctatttcg
     7441 tgatcgtcgg caccagcaac gcggtgaacc tgactgacgg actcgacggg ctcgcgatca
     7501 tgcccacggt gatgatcggc ggcgcactcg gcatcttcgc ctacgtcgcc gggcacgcgg
     7561 ttttcgccaa gtatctcggt tttccgtaca tacccggcgc gggcgagctc gcggtggtct
     7621 gcggcgcgat cgcgggcgcg ggcctcgcct tcctgtggtt caacgcctat ccggcggaag
     7681 tgttcatggg cgacgtcggc gcgcttgcgc tcggcgcggc gctcggcacc atcgccgtca
     7741 tcgtgcggca ggagatcgtg ctgttcatca tgggcggggt gttcgtggtg gagacgctgt
     7801 cggtgatgct gcaagtcgct tccttcaagc tcacgggcaa gcgcatcttc cgcatggctc
     7861 cgctgcatca ccactacgag ctgaagggct ggaaggagaa tcaagttgtc gtgcgctttt
     7921 ggatcataac gatgatgtta gttctcgttg gactttcgac actgaaactg cggtgagaag
     7981 tgaggagtaa ggagtgagga gtgaggcgtg acaggcttaa ccaggcggat ggctgggatt
     8041 tccccctcac cccttacccc tcacgcttca cggtaatcgg cgatgctgga cttgaatgga
     8101 cgaagggtag tcgtgctggg actcggtgat acgggactgt cgatggcgcg ctggctcgca
     8161 cggcgggggg ccgacgtgcg cgtcgccgat agccggaagg cgccgccgca ggcgccggcg
     8221 ctcgcgcgcg cgctgccgca ggtgcggctg gaaaccggcg ccttcagcga tgacaccatg
     8281 aaagacgcgg acctgatcgc gatcagcccc ggcatcgacc gccgcgaggc gccgattgca
     8341 agtgcgatcg agcgcggcgt gccggtagtg ggcgacgttg aattgttcgc gcaagccctc
     8401 aagaccctca tccctccgaa ggtcctggcg gtgaccggct ccaatggcaa gagcaccgtg
     8461 acggcgatgg cgggcgacat gtgccgcgcg gcgggtcgcg agacggtggt ggccggcaac
     8521 atcggcgtgc cggtgctgga tgcgctaacg gcgatcgagg agcgcggcac cgcaccggag
     8581 gtattcgtgc tcgagctctc cagtttccag ctcgagagca cggcgagcct tcagccggac
     8641 gcggcgaccg tgctcaacgt gaccgaggac catctcgacc gctatgacgg catggccgat
     8701 tacgcggccg cgaaagcgcg cgtgttctcg ggcaacggcg tgcaaatcct caatcgcgat
     8761 gaccgttcga ccctgaacat ggcacgcgcc ggccgcaccc tcttcacctt cgggttctcc
     8821 gctccgcgcg gggaatcgga gtgggggatc acgcccgagg gcggcgcccg cgcgctggcg
     8881 cacggcggcc gtcggctgat ggcggtggcg gagctcccgc ttgccggcct gcacaacgcc
     8941 gccaacgcgc tcgccgccca tgcgctcgcg cacgcgatcg ggctgcccga cgggccgctc
     9001 atcgacgcgc tgcgacggtt ccgggggctg ccccaccgca tgcggaaggt ggccgagatc
     9061 gcgggcgtcg ccttctacga cgattccaag ggcaccaacg tcggcgccac cgtggcggcg
     9121 ctctcgggcc tcgcgcagcc ggtggtgctg atcgccggcg gcgacggcaa ggggcaggac
     9181 ttctcgccgc tcgcgcccgc cgtgaaagcg cgcgcgcgcg ccgtggtgct gatcggccgc
     9241 gacgccgcgc tcatcgagcg cgcgattgcc ggcagcgggg cgcggatcga gcgcgccgcc
     9301 ggcatggatg aagcggtgga cgtggctgcc cgcgcggcgc agccgggcga cgccgtgctg
     9361 ctgtccccgg cgtgcgcgag ctacgacatg ttccgcgact acgtgcaccg ggcggaggtt
     9421 tttgccgcag cggtgcggcg tctcgaggac cggcggagat agagcgtgtt ttacaccgac
     9481 gtcaaaccgc gcgcacccat cgccgagtac gaccagagcc tggtatggac cgcggtgctg
     9541 ctgatcgggc tcggactcgt gatggtgtac tcggcgtcga tcgcgatcgc ggagggcagc
     9601 cgcgccaccg ggaatcagcc gacgtattac ctgatgcggc acggattgtt cgtggcggtg
     9661 ggcatcgtgg cgtccgtgat cgcgttccag attcccctgc gcctgtggca gcaggcggcg
     9721 ccgttcctgt tcctgttcgg caccggattg ctgatcctgg tgctggtacc gggcgtgggc
     9781 cgggaggtga acggcagcca gcgctggctt tcgctgcact tcgtgaccct gcagccttcc
     9841 gagttcgtga agctcgccgc ggtgctctac gccgccgact acacggtgag gaaggccgcc
     9901 cacatggcga gcttccgcaa ggggtttctc ccgatgttcg tggtgatgct ggtcgtcgga
     9961 gggctgttgc tgcgcgaacc ggatttcggg gcctttgtcg tgatatccgt gctcgcgctg
    10021 gggattctct tcctcggcgg catgaactgg aagctgttcg cggggttgtt cggcctgctg
    10081 ctggtcggat tcgtggcgct gatcctcgct tcgccctacc gcctgcagcg cgtgagcggg
    10141 ttcattgacc cgtgggccga cccttacggc aggggctatc agctttcaca cgcgctcatc
    10201 gccttcgggc gcggcgaatg gctgggcgtg ggcctcggcg ggagcgtcga gaagctgttt
    10261 tatctccccg aggcgcatac cgatttcctg ctcgcggtga ttgccgagga gctcggcttt
    10321 gcgggaatgg ccgccatcat cctcgccttc acctggatca tcgcacgggc gttcatgatc
    10381 gggcggcgcg cggcctccct tgagcgccat ttcccggcct tggtggcgca gggcatcggc
    10441 ctgtggattg gcgtgcaggc gatcatcaac atcggcgtca acacgggaat gctgccgacc
    10501 aagggactga ccctgccgct ggtttcgttc ggcggcagcg cgctggtttc gacttgctgc
    10561 gcgctcgcgg tactgctgcg agtcgactgg gagaaccggc agctgatgag aggtttcacc
    10621 gtttagtgat gagtgatagg tgatggggga tgagcggaaa aaccccggcg cgccgcggac
    10681 catcatgatc atggccggcg gaacgggcgg acatatcttc ccggccttgg cggtggcgga
    10741 gcacctgcgc gcgctgggct ggaacgtggt atgggtcggg gtgcgcggcg gcatggagga
    10801 gcggctgatt ccaccacgcg gctacaccct gtcgtggatc cgcttctccg gcgtgcgcgg
    10861 gaggggcatc gtcgcggccg cgttgctgcc gctcaacctg ttgatcgcgt tctggcagac
    10921 ggcgcggtcg atcttcgcgc accgtcccga cgtggtgctc ggcatgggcg gctacgtctc
    10981 gtttcccggc ggcatgatgg cttccttcct caaccgcccg ttcgcgatcc acgagcagaa
    11041 ctcggtggcg ggactggcga accgcgtgct ggcgaagctc gccgaccggg tgctcacctc
    11101 cttccccaac gcgttcggcg aggcgaccgc ggtgatctgg accggcaacc cggtgcgcga
    11161 ggaaatcgcg gccatggcgc cgccggagaa ccgctatgcg gcgcgcgccg ggcagttgcg
    11221 gctgttggtg gtgggcggca gccagggcgc gcaggtgctc aacgccgtcg tgcccgaggc
    11281 gctcgcgctg atcgccccgg aagtgcgccc gcgcgtcagg caccaggcgg gggcggtgca
    11341 ccacgacgcg gtgcgggccc gctaccaggc gcttggcgtc gccgccgacg tcgtcgcctt
    11401 catcgacgac atggcgggcc ggtacgcgga aacggacctg atcgtctgcc gcgccggcgc
    11461 caccaccgtc gccgagatcg cggcggcagg catcgcctcg gtgctggtgc cgtaccccca
    11521 ttcggtggac gaccaccaga cgatcaatgc gcgctttctc gccgaccgcg gcgcggcgct
    11581 gctgattcca cagcgcgaac tgacggcgcg gcggctggcc gaagtgcttt ccggattcac
    11641 gcgcgagcgc ctgctcgcca tggcgcagtc ggcgcgcgcc gccggcaggc cggaggcgac
    11701 gcgcgtggtg gcgcaagcct gtatggagct ggcggcatga agatacaagg cggaaggcgg
    11761 aaggcggaag gcggaggaaa cggcgggcgg agcgacgcga gatgagacac agagtcaaac
    11821 acgtgcattt cgtggggata ggggggaccg ctgtgagcag ggagcagggg cccctctggg
    11881 gatgcgaccg gccgcgagcg gcgtcggccc ccgggtttgt tgcgcagggt actgcggacc
    11941 attggcgatg agacacagag tcaaacacgt gcatttcgtg gggatagggg gcgcgggaat
    12001 gagcggcatc gccgaggtgc tgttgacgct ccgctacaag gtgagcggct ccgatcttgt
    12061 tgagaacgcc tcgacccggc gactgcggca gctcggcgcc aaaatccaca tcggccacga
    12121 cgaagcgcac atcgccgggg cggacg
//
LOCUS       DBNGWZ010000543         4036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2974851, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000543 DBNGWZ010000000
VERSION     DBNGWZ010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2974851"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="AC16CX_16940"
     CDS             complement(<1..1182)
                     /locus_tag="AC16CX_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014230.1"
                     /translation="MDQPTALRGAAVPAPAQSPWLLEVEDLHVHFVTTRGVVRAVEGI
                     SYKVRPGETVALVGESGCGKSVSSLAIMRLLAKPAGRIVAGRILFQGRNLLDLSEDEM
                     REIRGRDIAMIFQEPMTSLNPVLTIGFQIMEPLLIHLGMTVDAARARAVELLKLVGLP
                     DPERRLDQYPHQFSGGMRQRVMIAIALSCNPKLIIADEPTTALDVTIQAQILKLMKDL
                     SRDLGIAMVIITHNLGIVARYANRVNVMYAARLVEQGSAANVFAKPLHPYTAGLLRSV
                     PRLDQPRGLKLETIEGLPPNLLDPQPGCRFAPRCPAQQPACVSAPPPLAQVEPHRYSA
                     CIRAREMAQLGPTGLRLQSANPQPPVVKALDKAHPLLKVHDLKTYFEVGAGLKLIKGA
                     RL"
     gene            1482..2087
                     /gene="gstA"
                     /locus_tag="AC16CX_16945"
     CDS             1482..2087
                     /gene="gstA"
                     /locus_tag="AC16CX_16945"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HIR3014231.1"
                     /translation="MKLYYYPGACSMAVHIVLREAGFKFELDRVDLAAKKTESGEDYL
                     AVNPKGYVPALRLDDGTVLTEAGVIIQYLADRKPRRGLAPKPRTPERYKLMEWINFTA
                     SEIHKGLGAFFNPKMTPEWREAQLEYVGRRFGYLEKVLGGNHYLMGEFSIADAYLFTV
                     LNWTNFHRIDTSGWPNIQAFMRRIAERPSVKKAMKAEGLAK"
     gene            2201..2884
                     /locus_tag="AC16CX_16950"
     CDS             2201..2884
                     /locus_tag="AC16CX_16950"
                     /inference="COORDINATES: protein motif:HMM:NF023984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HIR3014232.1"
                     /translation="MFALYSGAPSRDWRTASREPVGLAPDPAATPEAVVQVYAARTWG
                     WRGYFGVHTWIAVKPAGARAYTVYEVIGWRLRYSESALAIRSRAPDERWFGNAPVLLA
                     DKRGPGVEALIERIDRAARDYPYAGEYSVWPGPNSNTFTAWVARAVPELEVDLPPTAI
                     GKDYLGDRLVASAPSGSGLQLSLFGLLSVTASGVEGLELNLLGLTFGVDPFAPALKLP
                     VLGRVGAAR"
     gene            complement(3029..3913)
                     /locus_tag="AC16CX_16955"
     CDS             complement(3029..3913)
                     /locus_tag="AC16CX_16955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014233.1"
                     /translation="MSSTLSETLCSSSLYRRSRILREVPQVPSRPAKGEVFTSGGSGS
                     IGAGIAAKAVPDGYTYLFVFDTHAVNPALIPNLPFDTTKDLASVMLVGTAPMAVVTNP
                     GKPYRSFGDVIKAAKAKPDALTYGSVGSGSLGHLSMILLQQAGGFKVVHVPYKGGGPM
                     IVDTTGGHVETGIASVAVLSPHVRGGKLRALAITGNKRTHAMPDVPALAEQGFPGFNA
                     LAWWGIFTPAGTPKPVLDKMHAELVKVFNLPDVRKQLGETLGMDLVVSTPEAQQKFLV
                     SEIARWGKVVREHSIRAD"
     gene            3801..4036
                     /locus_tag="AC16CX_16960"
                     /pseudo
     CDS             3801..4036
                     /locus_tag="AC16CX_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427887.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
BASE COUNT          697 a         1314 c         1331 g          694 t
ORIGIN      
        1 gagccgcgcg cccttgatca gcttgagacc ggcgccgact tcgaagtagg tcttgaggtc
       61 gtgcaccttc agcagcggat gcgccttgtc gagcgccttc accaccggcg gctgcgggtt
      121 ggcgctctgc agtcgcagcc ccgtcggccc cagttgcgcc atctccctcg cccggatgca
      181 cgccgagtag cgatgaggct cgacctgggc gagcggcggc ggcgcggaga cgcaggccgg
      241 ctgctgcgcc gggcagcgcg gggcgaaacg gcaccccggc tgggggtcga gcaggttggg
      301 cggcagaccc tcgatggtct cgagcttcaa gccgcgcggc tggtcgagcc gcggcaccga
      361 gcgcagcagt cccgcggtat agggatgcag gggcttcgcg aacacgttgg cggcgctgcc
      421 ttgctcaacc agacgcgcgg catacatcac gttcacccgg ttggcgtagc gcgcgacgat
      481 gccgaggttg tgggtgatga tcaccatcgc gataccgaga tcgcgcgaga gatccttcat
      541 gagcttcaag atctgcgcct ggatcgtgac gtcgagcgcg gtggtcggct cgtcggcgat
      601 gatgagcttg gggttgcacg agagcgcgat cgcgatcatc acgcgctggc gcatgccgcc
      661 ggagaactgg tgcggatact ggtcgagccg tcgctccggg tcggggaggc cgaccagctt
      721 gaggagctcg accgcgcggg cgcgcgccgc gtccaccgtc atcccgagat ggatcagcag
      781 cggctccatg atctgaaagc cgatggtcag caccgggttg agcgacgtca tcggctcctg
      841 gaagatcatc gcgatgtcgc gaccgcggat ctcgcgcatc tcgtcttccg agagatcgag
      901 caggttgcgg ccctggaaca ggatgcggcc ggcgacgatg cggccggcgg gcttcgcgag
      961 cagccgcatg atggcgagcg aggacaccga cttgccgcag ccggattcgc cgacgagcgc
     1021 gaccgtctcg cccggcctga ccttgtagga aatgccctcg accgcgcgca ccacgccgcg
     1081 cgtcgtcacg aaatgaacgt gcaggtcctc gacttcgagc agccagggac tttgcgcggg
     1141 cgcaggcacg gccgcgcctc gcagggcggt cggttgatcc atcctccggt gggtgccgct
     1201 tgtagcagtc ggacgaggct atgtgaccac gatgtggatt gtcaagtcac attaaagccg
     1261 gcgctgcgcg ccggtttaat tccgcgcgtt tgaacggaca gggggttgcc gttcaacgcg
     1321 cggtaacgca gggatgcagt tcagagaact caatatgata gacccagtct aatcaccggc
     1381 cctggccctt atttgccaag cgcagtgatc caccgaatac tatcggccgg gagatccgac
     1441 tcacgactca cctctcacga ctcacgcatt caggagaatg aatgaagctc tactactacc
     1501 ccggcgcctg ctccatggca gtgcacatcg tgttgcgcga agcaggattc aagttcgagc
     1561 tcgacagggt cgatctcgcg gcgaagaaga ccgagagcgg cgaggactac ctcgcggtga
     1621 accccaaggg ctacgtgccg gcgctaaggc tcgacgacgg cacggtcctc accgaggccg
     1681 gcgtcatcat ccagtatctc gccgaccgga aaccgcgccg gggcctcgcg cccaagccgc
     1741 gcactccgga gcgctacaag ctgatggaat ggatcaactt caccgcgtcc gaaatccaca
     1801 aggggctcgg ggcattcttc aatccgaaga tgacgccgga gtggcgtgaa gcgcagctcg
     1861 agtacgtcgg caggcgtttc ggctatctcg agaaagtgct cggcggcaac cactacctca
     1921 tgggcgagtt cagcatcgcg gacgcctacc tgttcaccgt gctcaactgg acgaatttcc
     1981 acaggatcga cacctcgggc tggccgaaca tccaggcgtt catgcgccgc atcgccgaac
     2041 gcccgagcgt caagaaggcg atgaaggccg agggcctcgc aaaataattg aaacgtggat
     2101 cgtgattcgt gagtcgtgat tcgataaccc aagtttgatc accatgagga cgcgccgcat
     2161 tattcaggtg ttttctcttt tcctaatttc aggagcggtc atgtttgcgt tgtattccgg
     2221 cgcgccttcc agggactggc gcaccgcaag ccgcgagccg gtcggcctcg cgcctgatcc
     2281 ggcggcgacg ccggaggccg tcgtgcaggt ttacgccgcc cgcacctggg gctggcgcgg
     2341 ctacttcgga gtgcacacct ggatcgcggt gaagcccgcc ggcgcgaggg cctacacggt
     2401 ctacgaggtg atcggctggc gcctgcgcta cagcgagtcg gcgctcgcga tccgcagccg
     2461 cgcgcccgac gagcgctggt tcggcaatgc gcccgtcctg ctcgccgaca agcgcgggcc
     2521 cggcgtggag gcgctgatcg agcgcatcga cagggctgcg cgcgactatc cctatgccgg
     2581 ggaatacagt gtctggccgg ggccgaattc caacacgttc accgcgtggg tcgcccgcgc
     2641 ggtccccgag ctcgaggtgg acctgccgcc gacggcgatc ggcaaggact acctcggcga
     2701 ccggctggtg gcgagcgcgc cgagcggcag cggacttcaa ctgtcgctct tcggtctcct
     2761 cagcgtgacg gcaagcggcg tcgagggcct cgagctcaac ttgctcggtc tcaccttcgg
     2821 cgtggatccg ttcgcgcctg cgctcaagct gccggtcctc gggcgcgtgg gcgcagcccg
     2881 ctagcccgta acagcgtgaa tcatgattcg ataacccaag tttgcagtgt gcagtaagca
     2941 gtttgcggct gctgcaaact gaccgctgca actgccaact tcggggccga gccgccagtc
     3001 acggctcttc ggccacgctt ctccctcgct agtcggcgcg gatgctatgc tcgcgcacga
     3061 cctttcccca gcgtgcgatc tccgacacga gaaacttctg ctgcgcctcc ggcgtgctca
     3121 ccacgaggtc catgccgagt gtctcgccga gttgcttgcg tacgtcggga agattgaaca
     3181 ccttcaccag ttccgcgtgc atcttgtcca ggacgggctt gggggtgccg gccggggtga
     3241 agatgcccca ccaggcgagg gcgttgaatc cggggaagcc ctgttctgca agcgccggaa
     3301 cgtccggcat cgcgtgcgtg cgcttgttgc cggtgatggc gagcgcgcgc agcttgcccc
     3361 cgcgcacgtg tggcgagagc accgccaccg aagcgatgcc ggtttcgacg tggccaccgg
     3421 tcgtgtccac gatcatcggc ccgccgccct tgtacggcac atgcaccacc ttgaagccgc
     3481 ccgcctgctg aagcaggatc atcgagaggt gccccaggct gccgctgccg accgagccgt
     3541 aagtcagcgc gtccggcttc gccttcgccg ccttgatcac gtcgccgaaa ctcctgtacg
     3601 gcttgcccgg gttggtcacg actgccatcg gcgcggtgcc gaccagcatc acggaggcga
     3661 ggtccttcgt ggtatcgaac ggcaggttcg ggatcagcgc cgggttgact gcgtgcgtgt
     3721 cgaacacgaa gagataggta tagccgtccg gcaccgcctt tgcggcgata cccgcgccga
     3781 ttgaacccga gccgccggag gtgaacacct cgcccttcgc cgggcgtgaa ggcacctggg
     3841 gcacctcgcg caggatccgc gagcgcctgt acaaggagct cgagcacaac gtctcgctca
     3901 aggtggacga cactgacagc gccgatacct tcctggtctc cgggcgcggc gagctgcacc
     3961 tggccatcct gatcgagacc atgcgccgcg agggctacga gtttcaggtc tccaagccgg
     4021 aagtcatcta ccgcac
//
LOCUS       DBNGWZ010000544         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2092372, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000544 DBNGWZ010000000
VERSION     DBNGWZ010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2092372"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="AC16CX_16965"
     CDS             <1..836
                     /locus_tag="AC16CX_16965"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014234.1"
                     /translation="LGNAGTFAAGLQQAFRSQAMAKPLHGGHAAEAGALAALCALQGV
                     TGVADILEGEVGFGAAMSVNPDWSRATRGLGTDYHITRITFKNHGCCGHNFASLDAAL
                     QLKRQHAFTHKDIRRVRIATYQGGLDIVDNLHPEGDYQAKFSIQYTVAHALVHGSVRL
                     NAFVPERLGDPDVRALMQKIECVADPELSKAFPRQRAARVEIELADGRKLAHFQPTRK
                     GDPEMPLSDEELNDKFMELAAPVVGNAGARVLLDRLWKLETEKSMDFEPSERAVVHAT
                     S"
     gene            970..>2108
                     /locus_tag="AC16CX_16970"
     CDS             970..>2108
                     /locus_tag="AC16CX_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3014235.1"
                     /translation="MPMPDPKLPLSRYTVLDLTSARSGPTAVRQLADWGANVIKIEDP
                     ALQDGGSGPWDRRHGSDFQNMQRNKRSLTLNLKTPEGVAIFKRLADQADVIVENFRPR
                     VKHRLGIDYETLKKTNPRLIYASISGFGQEGPYRDRPGLDHVVQGMSGLMSVTGLPGQ
                     GPVRVGIAISDSSSGIYCAFGIVLALLEREHSGEGQWVNTSLLQALIAMLDFQAARWL
                     IAHEVPPQAGNDHPTAIPTGMFKTADGYINIAASGKLYERLCKALGAPELFSHPDYCE
                     YRLRSKNRVALNAEIEKRTVKRSSAEWIDLLNEAGVPCGPIYTIDKMFADPQVQTLGM
                     AHPVVHPKLGQQELLGQGVNLSRTPAQLRTHAPDAGEHTREILGEL"
BASE COUNT          386 a          722 c          665 g          335 t
ORIGIN      
        1 cgctcggcaa cgccggcact ttcgccgcgg ggctgcaaca ggcgttccga tcgcaagcga
       61 tggcaaagcc gctgcacggc gggcacgcgg cggaggccgg tgcgctcgcc gcgctgtgcg
      121 ccctgcaagg cgtcaccgga gtcgccgaca tcctggaagg cgaagtgggc ttcggcgccg
      181 cgatgagcgt caaccccgac tggtcgaggg ccacgcgcgg cctcgggacc gactatcaca
      241 tcacgcggat caccttcaag aatcacggct gctgcggcca caatttcgcg tcgctcgacg
      301 ccgcccttca gctcaagcgc cagcatgctt tcacgcacaa ggacatccgc agggttcgca
      361 tcgcgactta ccagggcggg ctcgacatcg tggacaacct gcatcccgag ggcgattacc
      421 aggcgaagtt cagcatccag tacaccgtcg cgcacgccct ggtccacggc agcgtgcgcc
      481 tgaacgcctt tgtccccgag cgtctcggcg acccggatgt gcgcgcactg atgcagaaga
      541 tcgagtgcgt ggccgacccc gagctctcga aggcttttcc gcgccagcgc gccgcgaggg
      601 tcgagatcga gctcgccgac gggcgcaagc tcgcccattt ccagcccacg cgcaagggcg
      661 acccggagat gccgctctcc gacgaggagc tgaacgacaa gttcatggaa ttggcggcgc
      721 ctgtggtggg caacgccggc gcccgcgtgc tgctcgaccg cctgtggaag ctcgaaaccg
      781 agaagtccat ggacttcgag ccaagtgagc gagcggtcgt gcacgccacg tcatgaccgg
      841 tgagcgttca cgtgagggcg tcagagcgtg agagggtgaa agcgattttt ccacctcccg
      901 atttcacgat ctctcgattt cacggactac ttcgacttgc gtgccgcgac tcatactcta
      961 aatatcgcta tgccaatgcc tgaccccaag ctcccattaa gccgttacac cgtcctcgac
     1021 ctgaccagcg cccgctccgg tcccaccgcc gtgcgccagc tcgccgactg gggcgcgaac
     1081 gtgatcaaga tcgaggaccc cgccttgcag gacggcggca gcgggccgtg ggaccggcgc
     1141 cacggctcgg acttccagaa tatgcagcgg aacaaacgaa gcctcacgct caacctgaag
     1201 acgccggaag gcgttgcgat cttcaagcgc ctcgccgacc aggcagacgt catcgtcgag
     1261 aactttcgcc cgcgcgtgaa acaccgcctc ggcatcgact acgaaaccct gaagaagacc
     1321 aacccgcgcc tgatctacgc cagcatctcc ggcttcggcc aggagggccc ctaccgcgac
     1381 cggccgggcc tggaccacgt ggtccagggc atgagcgggt tgatgtccgt caccgggctt
     1441 ccggggcagg gtccggtgcg cgtgggcatt gcgatttccg actcgagttc gggcatctac
     1501 tgcgcgttcg gcatcgtgct cgcgctgctc gagcgcgagc actcgggcga gggtcagtgg
     1561 gtgaacacct cgctcctgca ggcgctgatc gcgatgctcg atttccaggc cgcgcgctgg
     1621 ttgatcgctc atgaggtccc gccgcaggcc ggcaacgacc atcccaccgc gattccgacc
     1681 ggcatgttca agaccgcgga cggctatatc aacattgcgg cctccggcaa gctgtacgag
     1741 cggctgtgca aggccctcgg cgcgccggag ctgttcagcc acccggatta ctgtgaatac
     1801 cggttgcgct caaagaaccg ggtcgcgctc aatgcggaga tcgagaagcg taccgtcaag
     1861 cgctcctccg ccgagtggat cgatttgctc aacgaggccg gcgtgccgtg cggcccgatc
     1921 tacaccatag acaagatgtt cgcggatccg caggtgcaga cactcggcat ggcgcacccg
     1981 gtcgtgcacc ccaagctcgg gcagcaggag ctgctcggcc agggcgtcaa cctctcgcgc
     2041 acgccggcgc agttgcgcac tcacgcgccc gacgccggcg agcatacgcg cgagatactg
     2101 ggcgagct
//
LOCUS       DBNGWZ010000545         5994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2342436, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000545 DBNGWZ010000000
VERSION     DBNGWZ010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2342436"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..875)
                     /locus_tag="AC16CX_16975"
     CDS             complement(144..875)
                     /locus_tag="AC16CX_16975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3014236.1"
                     /translation="MTAGVVLRSTLFALFQLVITPPYALIALLTFPLSPFTRYRVIST
                     WARIMVAASEKICGIRYCVLGADRFPPPPFIVLSKHQSAWETLAFQLIFPPQVWVVKR
                     ELLWIPFFGWGLAMLSPIAIDRSAGTRALRQLLEQGRDRLAQGFCVVIFPEGTRVAPG
                     ASREYQIGGAWLATRTATPVVPVAHNSGEVWRRKAFLKYPGTITVSIGAPIETGSLTP
                     EELNRRVKDWIEGEMRRIKAVSGER"
     gene            complement(872..1405)
                     /gene="gmhB"
                     /locus_tag="AC16CX_16980"
     CDS             complement(872..1405)
                     /gene="gmhB"
                     /locus_tag="AC16CX_16980"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HIR3014237.1"
                     /translation="MKLVILDRDGVINFDSDAYIKSPDEWRPIPGSLDAIARLTQAGY
                     HVVVATNQSGVGRGLFEMATLNAIHDKMHRAVGQAGGRIDAVFFCPHAQEANCDCRKP
                     KPGLLEEIGRRFNASLKNVPCVGDSQRDLEAAAAVGALPVLVLTGKGEATRRENRLPP
                     GTRVFKDLAGAVKSLVK"
     gene            complement(1402..3474)
                     /gene="glyS"
                     /locus_tag="AC16CX_16985"
     CDS             complement(1402..3474)
                     /gene="glyS"
                     /locus_tag="AC16CX_16985"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3014238.1"
                     /translation="MKANKPAGAEKTLLVELLTEELPPKLLRTLGDAFADQLVAEIVK
                     HRLRDRIPTKQVFATPRRLAVLIPEVRVKALESAHSVEGPASSNIKAVEGFARKHKVT
                     PESLERVQTEKGEIVVARFTLPGVHLDAVLTNIVEGVLKKLPIPKMMRWGSGNAQFAR
                     PVHGLVMLHGSRVVPGEVLGVKSSNRTVGHRFLSGSAIMLKRADDYERALREKGRVIV
                     DFAERRAGIARQLEKTSGGAKPVVDDALLDEITALVETPAAYVGEFGPEFLAVPEECL
                     ILSMQQHQRYVPLRDGKTGKLLPRFLFVSNLETKDPREIIHGNERVLHARLADAKFFH
                     DQDLKTRLEARVPRLASVVYHNKLGSQLERVERIQLLAGRIARALGADSLVAERAAWL
                     SKADLLTDMVGEFPELQGTMGRYYALHDGEPKAVADAIGSHYQPRFAGDAVPDGAVGA
                     AVALADKLDALAGLFGIGQVPTGDRDPFGLRRAALGVIRILVERKLALPLGELVDAAF
                     KGYNGRVGDAHTDLESFIYDRLSGHLKEAGYTTLEVESVVSLRPAQVHLVPRQLEAVR
                     AFNKLPEAESLAAANKRVVNILKQATTKGESFAKAAPEMLKEQAERLLFETLQSTSRR
                     AASLFEQGDFTGYLKSFAVLKSPVDAFFDSVMVMVEDRELRQNRLALLSDLRNEMNRV
                     ADISKLAK"
     gene            complement(3487..4392)
                     /gene="glyQ"
                     /locus_tag="AC16CX_16990"
     CDS             complement(3487..4392)
                     /gene="glyQ"
                     /locus_tag="AC16CX_16990"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3014239.1"
                     /translation="MLTFQQIILKLNQYWDRHGCVLLQPYDMEMGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVMKPSPAKFQDVYLGSLKDALG
                     INPRKNDIRFVEDDWESPTLGAWGLGWEVWLNGMEITQYTYFQQVGGLDCRPVLGELT
                     YGLERLAMYLQGKESVFDLVWVDGVTYGDVYHQNEVEQSRYNFELANTAMLFQHFGQF
                     EAEAKRLMEAQCVLPAYEMVMKCSHTFNLLDARGAISVTERQAYIGRVRNLARLVAQA
                     YYESREKLGFPMLKSQPAAKKKARA"
     gene            complement(4663..>5994)
                     /gene="lnt"
                     /locus_tag="AC16CX_16995"
     CDS             complement(4663..>5994)
                     /gene="lnt"
                     /locus_tag="AC16CX_16995"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR3014240.1"
                     /translation="TLLFCGFLALFPAAVGYACSRPALPHAARYGLLAPAAWTLAEWT
                     RGWLFSGFPWIALGYSQVPASPLAGFAPVLGIHSVTLSTALSAGLLAVLWQRYTERDE
                     SGGRRAEGKTGKTSGASSFIPHPSSLIPRILLHPSSFILILLWLGGFGLKQVEWTEPV
                     GAPFKVSLLQGNVPQDLKWREDRVLATLDTYRALVRLSVSPLIVLPETALPLFLEQVP
                     RDYLEELASHARKNGGDILIGVPERLPGGEYFNSAVSFGTAPNQTYRKSHLVPFGEFI
                     PLRPVLGWVVGVLAIPLQDFSRGAENQRPLAVAGQRVAVNICYEDAFGEEIIRQLPEA
                     TLLVNVSNVAWFGRSIAPWQHLQISQARALETGRYMLRATNTGITGVIDPRGEVVAAA
                     PGFKMATVNHTVQGYSGSTPYVRWGNYAALALCVALIAAGGWLARRPARSA"
BASE COUNT         1081 a         1866 c         1956 g         1091 t
ORIGIN      
        1 ccgcgagcag cagggaaaga acgatcagcg gaaccagggc gcgcatggat cagatggtaa
       61 gcggtaagcg gtaagcagta aacagcatgc ggtgagcagt aagcggtaaa cggtaagcag
      121 tgaacggcaa gagctttttc cgctcaccgc tcaccgctca ctgctttaat gcgccgcatc
      181 tcgccctcga tccagtcctt gactctgcgg ttgagttcct cgggcgtgag gctcccggtc
      241 tcgatcggcg cgccgatgct cacggtgatc gtgcccgggt atttcaggaa cgccttgcgc
      301 cgccacacct cgccggagtt gtgcgcgacc ggcaccaccg gtgtcgcggt cctggttgcc
      361 agccaggcgc cgccgatctg gtattcgcgg cttgcgccgg gcgcgacccg ggtgccctcc
      421 gggaagatca cgacacagaa tccctgggcc agccggtcgc gcccctgctc gagcagctgg
      481 cgcagggcgc gcgtgccggc gctgcggtcg atcgcgatcg gcgagagcat ggcgagcccc
      541 cagccgaaga acggtatcca cagcaattcg cgcttcacca cccacacctg cggcgggaag
      601 atcagctgga aggcgagcgt ctcccacgcg gattggtgct tcgagagcac gatgaagggc
      661 ggtggtggaa aacggtctgc gccgagcacg cagtaacgga tgccgcagat tttctccgag
      721 gctgcgacca tgatccgggc ccaggtggaa atcacccgat agcgggtgaa aggggaaagc
      781 gggaaggtga gcagcgcgat cagcgcatac ggcggcgtga tgacgagctg gaacagcgcg
      841 aacagcgtgg agcgcaggac gacgccggcg gtcatttgac cagcgatttc acggctccgg
      901 cgaggtcctt gaacacccgc gtgccggggg gcagccggtt ctcgcgccgc gtcgcttcgc
      961 ccttgccggt cagcaccagc accggcaggg cgccgaccgc ggcggcggcc tccaggtcgc
     1021 gctgcgaatc gccgacgcag ggcacgtttt tcaggctggc gttgaagcgc cggccgatct
     1081 cctcgagcag cccgggcttg ggcttgcggc agtcgcagtt ggcttcctgg gcatgcgggc
     1141 agaagaacac ggcgtcgatg cgcccgcccg cctggccgac cgcgcggtgc atcttgtcgt
     1201 ggatggcgtt gagcgtcgcc atctcgaaca gcccgcgtcc cacgccggac tgattggtcg
     1261 ccaccaccac gtgataaccc gcctgggtga gccgtgcgat cgcgtccagg ctcccgggaa
     1321 tcggcctcca ctcgtcgggg ctcttgatgt aggcgtcgct gtcgaagttg atgacgccgt
     1381 cgcggtcgag gatgacgagc ttcatttcgc gagcttcgag atgtccgcga cgcggttcat
     1441 ctcgttgcga agatcggaaa gcagggcgag gcggttttgc cgcaactcgc ggtcttcgac
     1501 catcaccatg acggaatcga agaaagcgtc aaccggagac ttcaggaccg cgaagctctt
     1561 caagtagccg gtgaagtccc cttgctcgaa caggcttgct gcgcgccggg aggtggactg
     1621 gagcgtttcg aacagcagcc gttcggcctg ctctttcagc atttcgggcg cagccttggc
     1681 aaacgactcg ccctttgtcg tggcctgctt gagaatgttt accacgcgct tgtttgcagc
     1741 cgcaaggctt tcggcctcgg gcagcttgtt gaacgcgcgc accgcctcca gctggcgcgg
     1801 caccagatgg acctgggccg ggcgcaggct gacgacggat tcgacttcca gtgtcgtgta
     1861 tcccgcctcc ttgaggtggc ccgaaaggcg gtcatagata aaggattcga gatctgtatg
     1921 tgcgtcgccc acccgcccgt tgtatccctt gaacgccgca tcgaccagtt ccccgagcgg
     1981 gagcgccagt ttgcgctcaa cgaggatacg gatgaccccg agcgcggcgc gacgcagccc
     2041 gaaagggtcc ctgtcaccgg tcgggacctg cccgatgccg aacaggccgg cgagcgcgtc
     2101 gagtttgtcg gcgagcgcaa cggctgcacc gactgcgccg tcggggacgg catcgcccgc
     2161 aaacctcggc tggtaatgcg agccgatcgc gtccgcaacg gctttgggct cgccgtcgtg
     2221 cagcgcatag taacggccca tggtcccctg tagctcgggg aactcgccga ccatgtcggt
     2281 caggaggtcg gccttggaga gccaggctgc gcgttcggcg acaagagaat cggcgccgag
     2341 cgcgcgggcg attctgcccg ccagcaactg gatgcgctcg acgcgctcga gctggctgcc
     2401 gagcttgttg tgatagacga cgctggcgag ccgcggcacg cgggcttcca gccgcgtctt
     2461 gaggtcctgg tcgtggaaga acttggcgtc ggcgagccgc gcgtgcagca cgcgctcgtt
     2521 gccgtggatg atctcgcgcg ggtccttcgt ttccagattg gatacaaaca ggaaacgcgg
     2581 cagcagtttc cccgtcttcc cgtcgcgcag gggcacgtac cgctggtgct gctgcatgga
     2641 caggatcagg cattcttccg gcaccgcgag aaactcaggg ccgaattccc ccacatatgc
     2701 ggccggggtc tccaccaggg ccgtgatttc gtccagcagt gcatcgtcta cgacgggctt
     2761 cgcaccgccg gaggttttct caagttgcct cgcaatcccg gcccggcgct ccgcgaagtc
     2821 gacgattacc ctgccttttt cgcgcagagc gcgctcgtaa tcgtcagcgc gcttgagcat
     2881 gattgcgctg ccggaaagga aacggtgacc cacggttctg ttggagcttt ttacccccag
     2941 cacttcgcca ggaacgactc gcgaaccgtg cagcatcact agcccgtgca ccggccgcgc
     3001 gaactgtgcg ttgccgctgc cccagcgcat cattttggga attggcagtt tctttagaac
     3061 gccttcaaca atgttcgtta ggacggcatc aaggtgaacg ccgggtaaag tgaatctcgc
     3121 aacaacaatc tcacctttct ctgtctgaac ccgctcgagc gactccggcg tgactttgtg
     3181 cttcctagca aatccttcga cggcttttat gttcgaactg gcaggccctt caactgaatg
     3241 tgccgattcg agggctttca ctcgaacttc cgggattaga actgccagcc ttcgaggcgt
     3301 cgcaaacacc tgcttcgtgg gaatacggtc tctcagccga tgcttaacaa tttccgccac
     3361 taattgatca gcgaacgcat ctccgagcgt gcgtagcaat ttcggtggta gctcttcggt
     3421 tagcagctcg accagaagag ttttttccgc acccgccggc ttattggctt tcatgtcgga
     3481 catcgttcag gcacgagcct tcttctttgc cgccggctgt gatttcagca tggggaagcc
     3541 aagcttttcg cgggattcgt aataagcttg cgccaccagc cgcgcgagat tgcgcacgcg
     3601 accgatgtag gcctggcgct cggtcaccga aatcgcgccg cgcgcgtcga gcaggttgaa
     3661 ggtgtgcgag cacttcatca ccatctcgta ggcggggagc acgcattgcg cttccatcag
     3721 gcgcttcgct tccgcctcga actgtccgaa gtgctggaac agcatggcgg tgttggcgag
     3781 ctcgaagtta tagcgcgact gctcgacctc gttctggtgg tagacatcgc cgtaagtgac
     3841 gccgtccacc cacacaaggt cgaacacgct ttccttgccc tgcaagtaca tcgcaaggcg
     3901 ctcgaggccg taggtgagct cgcccagcac cggcctgcaa tcgagcccgc cgacctgctg
     3961 gaagtaggtg tactgcgtga tctccatgcc gttcagccac acctcccagc ccaggcccca
     4021 ggcgccgagg gtgggggatt cccagtcgtc ctcgacgaag cggatgtcgt tcttgcgcgg
     4081 attgatgccg agcgcgtcct tgagcgagcc gagatagacg tcctggaact ttgcgggcga
     4141 gggcttcatc accacctggt actggtagta atgctggagg cggttggggt tctcgccgta
     4201 gcggccgtcc ttgggccgcc gcgagggttg cacgtaggcc gcgttccacg gctccggccc
     4261 gatcgcgcgc aggaaggttg cggtatggaa ggtgcccgcg cccatctcca tgtcatacgg
     4321 ctgcagcagc acgcagccgt ggcggtccca gtactggttg agcttgagaa tgatctgctg
     4381 gaaggtcagc atcggtcgcg ggtgttccta acccgcgaat tttacaggct tccgcaggat
     4441 tttgtcggga ctgagcccga caaaatcctt aaccaagaac aagtcggaca ccgggtctag
     4501 gcaagaccgc cctggactgg atatctcatg aaagggtcgg cgcatccatc gttacggagt
     4561 tgattcacct aaatgaagta ctcaagcccg catgccgctg gttccttccc gcgcgatttg
     4621 attgcatgac ccccaaagcg tggcagtcat gcagcatgcg ggttaggcgg aacgcgctgg
     4681 gcggcgcgcg agccagccgc ccgctgcgat caacgcgacg cagagcgcga gcgcggcata
     4741 gtttccccag cgcacgtagg gcgtggagcc gctgtagccc tgaaccgtgt gattgacggt
     4801 tgccatcttg aacccgggcg cggcggcgac tacctcgcca cgcgggtcga tcacgccggt
     4861 gatgccggtg ttggtcgcgc gcagcatata gcggccggtt tccagggcgc gcgcctgcga
     4921 gatctgcagg tgttgccacg gagcgatcga gcgcccgaac caggcgacgt tgctgacgtt
     4981 gacgaggagg gtcgcttccg gcagctggcg gatgatctct tccccgaacg cgtcctcgta
     5041 gcagatgttg accgccacgc gctgccccgc caccgcgagc gggcgctggt tttccgcgcc
     5101 gcgcgagaag tcctgcaacg gtatggcgag caccccgacg acccatccca gcaccggccg
     5161 cagcgggatg aactcgccga acggcaccaa gtgcgacttg cgataggtct ggttcggcgc
     5221 ggtcccgaag gagaccgcgc tgttgaagta ctcgccgccc ggcaggcgct ccggaacgcc
     5281 aatcaggata tcgccgccgt ttttgcgcgc gtgggaggcg agttcctcga ggtagtcgcg
     5341 tggaacctgc tccaggaaca gcggcaaggc ggtttccggc aggacgatga gcgggctcac
     5401 ggaaaggcgg acgagggcgc ggtaggtatc aagggtggcc agcacgcggt cttcgcgcca
     5461 cttcaggtcc tggggcacgt tgccttgcag caggctcacc ttgaaggggg cgccgaccgg
     5521 ctcggtccat tcgacctgct tgagaccgaa gccgccgagc cagagcagga taaggatgaa
     5581 ggatgaagga tgaaggagga tgcgagggat gagggatgag ggatgaggga tgaaagacga
     5641 ggcgccgctt gtcttgccgg tttttccctc cgccctccgc cctccgctct catccctctc
     5701 ggtgtaacgc tgccacagca cggcgaggag tcccgcagat agcgcggtgg aaagcgtgac
     5761 gctgtgtatt cccagcaccg gcgcaaagcc ggcgaggggg cttgccggaa cctgggaata
     5821 tccgagtgcg atccagggaa aaccggagaa cagccacccg cgcgtccatt ccgccagcgt
     5881 ccaggccgcc ggcgccagca ggccgtagcg tgcggcatgc ggcagagcgg gcctggagca
     5941 tgcgtaaccg acggccgcgg gaaacagcgc gagaaacccg cagaacagga gagt
//
LOCUS       DBNGWZ010000546         3465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3225068, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000546 DBNGWZ010000000
VERSION     DBNGWZ010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3225068"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="aroG"
                     /locus_tag="AC16CX_17000"
     CDS             complement(<1..1018)
                     /gene="aroG"
                     /locus_tag="AC16CX_17000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HIR3014241.1"
                     /translation="MQYRTDDLRIKEIKELVPPSHVLREFPISEKAARTTFETRQAIH
                     RILHGADDRLLVIMGPCSIHDVKAAKEYAGRLKRERDRLAGELLVVMRVYFEKPRTTV
                     GWKGLINDPNLDGSFRINEGLRIARRLLLDLDEMDVPVACEFLDMITPQYIADLVAWG
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGNVKIAVDAIRAAHAPHHFLSVTKGGHS
                     AIVSTSGNEDCHVILRGGDKPNYDTAGVDAAAKDLAEGGVPARLMIDFSHGNSSKKPE
                     KQMDVARDVAGQIAGGEARIFGVMVESHLKAGRQDLVPGKELVYGVSITDGCIGWEDS
                     RKLLG"
     gene            complement(1176..2651)
                     /gene="tldD"
                     /locus_tag="AC16CX_17005"
     CDS             complement(1176..2651)
                     /gene="tldD"
                     /locus_tag="AC16CX_17005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HIR3014242.1"
                     /translation="MKPQDPQEARVLEREASFATANEVLLSPYALDANRLETVFGQIL
                     AHRVDYADLYFQYTRSESWSLEESIVKSGSFNIDQGVGVRAVSGEKTAFAYSDDISLT
                     ALEAAARATRAIARRGQAGRTQVAKRAAGHNLYVPHDPIASLPDPMKVALLERIERHA
                     RALDPRVTQVMAWLAGEYEVVLVARSDGVLAADVRPLVRLSLQVIVEQNGRREQGSSG
                     GGGRFDYAYFTDEVLGDYARKAVDQALLNLEARPAPAGTMTVVLGPGWPGVLLHEAVG
                     HGLEGDFNRKGTSAFSGRIGERVAAPGVTVVDDGTLARRRGSLNIDDEGHPTQCNVLI
                     EDGVLKRYMQDSLNARLMKVPPTGNGRRESYAHIPMPRMTNTYMLNGDKPREEIIASV
                     KNGIYAVNFGGGQVDITSGKFVFSASEAYAIENGRLAHPVKGATLIGNGPDALTRVAM
                     IGNDMALDPGVGTCGKEGQSVPVGVGQPTMRIDGLTVGGTA"
     gene            complement(2750..>3465)
                     /locus_tag="AC16CX_17010"
     CDS             complement(2750..>3465)
                     /locus_tag="AC16CX_17010"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3014243.1"
                     /translation="QGAKLVALPEYFAIMGLRDQDKVKLREKPGAGPIQEFLSTTARR
                     HGIWLVGGSVPLESSSPDKVRNSCLVYDDRGRLAARYDKIHLFGFEMGKERYDEALTI
                     EPGKEVVTVDSPFGRLGLSICYDLRFPELYRAMKPVDIILVPSAFTETTGSAHWETLI
                     RARAIENLAYVLAPAQGGYHLNGRETHGDSMIVDPWGVVLDRLPRGSGVVVAGVNLEH
                     LRNLRSSLPALTHRTLHRC"
BASE COUNT          545 a         1181 c         1119 g          620 t
ORIGIN      
        1 cgccgaggag cttcctgctg tcctcccagc caatgcagcc gtcggtgatg ctcacgccgt
       61 agacgagctc cttgcccggc accaggtcct gccggcccgc cttgagatgg ctctccacca
      121 tcacgccgaa gatgcgcgcc tcgcccccgg cgatctggcc cgcaacgtcc cgcgccacgt
      181 ccatctgttt ctcgggcttc ttgctcgaat tgccgtggct gaagtcgatc ataagccgcg
      241 ccggaacgcc gccctcggcg aggtccttgg cggcggcatc tacgcctgcc gtgtcgtaat
      301 tcggcttgtc cccgccgcgc aggatgacat ggcagtcctc gttgccggat gtggagacga
      361 tcgcgctgtg cccgcccttg gtaacggaga ggaaatggtg cggcgcatgc gcggcccgga
      421 tcgcgtccac cgcgatcttc acgttgccgt ccgttccgtt cttgaagccg acggggcacg
      481 acagtcccga ggcgagctcg cggtggatct gggattcggt cgtgcgcgcg ccgatcgcgc
      541 cccacgccac caggtcggcg atgtactgcg gcgtgatcat gtcgagaaac tcgcaggcca
      601 ccggcacgtc catctcgtcg agatcgagca gcagccggcg cgcgatgcgc aagccctcgt
      661 tgatgcggaa gctgccgtcg aggttggggt cgttgatgag gcccttccag cccacagtgg
      721 tgcgcggttt ctcgaaatag acgcgcatca ccaccagcag ctcgccggcg aggcggtcgc
      781 gctcgcgctt gagcctcccg gcgtattcct tcgccgcctt cacgtcgtgg atggagcacg
      841 gccccatgat caccagcagc cggtcatccg ccccgtgcag gatgcggtgg atcgcctgcc
      901 gcgtctcaaa ggtcgtgcgc gcggccttct cggaaatcgg gaactcgcgc agcacgtgcg
      961 acggcggcac gagttccttg atttccttga tccggaggtc gtcggtgcgg tattgcatga
     1021 tgccttctgc ctgtgaaatc aaacgattat agcaaaaggc tagcggtaag cagtaagcgg
     1081 caagcggcaa gcggtgcaat accaggaagc gtggcggcgc gagcgcgccg ttcactgctc
     1141 acggctcacg gctcaccgct gacggcttac tgaagttagg cagtgccgcc gacggtaaga
     1201 ccgtcgatgc gcatcgtggg ctggccgacg ccgaccggca cgctctgccc ctccttgccg
     1261 caggtgccca cgcccgggtc gagcgccatg tcgtttccga tcatcgccac gcgcgtgagc
     1321 gcgtccggcc cgttgccgat gagcgtcgcg cccttgacgg gatgcgcgag cctgccgttc
     1381 tcgatcgcgt acgcctcaga ggcggagaac acgaacttgc cgctggtgat gtccacctgc
     1441 ccgccgccga agttgacggc atagatgccg ttcttcacgg aggcgatgat ttcctcgcgc
     1501 ggcttgtcgc cgttcagcat gtaggtattg gtcatgcgcg gcatcggaat gtgcgcgtac
     1561 gactcgcgcc ggccgttgcc ggtgggcggg accttcatca ggcgcgcgtt caggctgtcc
     1621 tgcatgtaac gcttcagaac gccgtcctcg atcaacacgt tgcactgagt gggatggcct
     1681 tcgtcgtcga tgttgagcga gccgcggcgg cgcgcaagcg tgccgtcatc caccacggtg
     1741 acacctggcg ccgccacccg ctcgccgatg cgcccgctga acgcggaggt gcccttgcgg
     1801 ttgaagtcgc cttcgaggcc gtggcccacc gcttcgtgca gcagcacgcc cggccagccc
     1861 gggccgagca ccacggtcat ggtgcccgcc ggggcgggcc gcgcctccag gttgaggagc
     1921 gcctggtcca ccgccttgcg cgcgtagtcc ccgagcactt catcggtgaa ataggcgtag
     1981 tcgaagcgcc cgccaccgcc cgagctaccc tgctcgcgcc ggccgttttg ctcgacgatc
     2041 acctggagcg aaagccgtac cagcggccgc acgtcggcgg cgagcacgcc gtccgagcgg
     2101 gcgaccagca ccacttcata ctcgcccgcg agccaggcca tgacctgggt cacgcgcgga
     2161 tcgagcgcgc gcgcgtgacg ctcgatgcgc tcgagcagag caaccttcat cgggtccggg
     2221 aggctcgcaa tcggatcgtg cggcacgtag agattgtgcc ccgcggcgcg cttcgccacc
     2281 tgggtgcggc ccgcctggcc ccggcgggca atcgcccgcg tcgcacgggc cgcggcttcc
     2341 agcgcggtca ggctgatgtc gtcggaatag gcgaacgccg tcttctcccc gcttaccgcg
     2401 cgcacgccta cgccctggtc gatgttgaag ctaccggact tgacgatgct ctcctccaag
     2461 ctccaggact cgctgcgcgt gtactggaaa taaaggtcgg cgtagtccac ccggtgcgcg
     2521 agtatctggc cgaataccgt ctccagccgg ttcgcgtcga gcgcatacgg cgagagcaaa
     2581 acttcgttcg ccgtggcgaa cgaggcttcg cgctcgagga cacgggcttc ctgtggatct
     2641 tggggcttca cgggtatcca gtatatatgg aggccgggta tccgccttca atctcagaat
     2701 cgatcttccg gatcacggat caccgatcac ggctcacgct gttcctgtat cagcagcgat
     2761 ggagggtccg gtgcgtcaac gccggcaggc tgcttcgcag gttgcgcagg tgctccaggt
     2821 tgacgccggc caccaccacc ccggagccgc gcggcagccg gtccagcacc actccccaag
     2881 ggtccacgat catgctgtcg ccgtgggttt cgcgtccgtt gaggtgatag ccgccctgcg
     2941 ccggggcgag cacgtaagcg aggttctcga tggcgcgcgc ccggatcagc gtctcccagt
     3001 gcgcgctccc ggtggtctcg gtgaatgcgg aaggcaccag gatgatgtcc acgggtttca
     3061 tcgcgcggta gagctcgggg aagcgcagat cgtaacagat ggaaagcccc agccttccga
     3121 acggcgaatc caccgtcacc acctccttgc cgggctcgat ggtaagcgct tcgtcgtagc
     3181 gctccttgcc catctcgaag ccgaagagat ggatcttgtc gtagcgcgcg gcgagcctgc
     3241 ccctgtcgtc gtaaaccagg cagctgttgc gcaccttgtc agggctcgaa gactcgagcg
     3301 gcaccgagcc cccgacgagc cagatgccgt gcctgcgggc cgttgtcgag aggaattcct
     3361 ggatcgggcc cgcgcccggc ttctcccgca acttgacctt gtcctggtcg cgcaagccca
     3421 tgatcgcgaa atactcggga agcgccacca gcttcgcgcc ctgcg
//
LOCUS       DBNGWZ010000547         2165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_703440, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000547 DBNGWZ010000000
VERSION     DBNGWZ010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_703440"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="AC16CX_17015"
     CDS             complement(<1..544)
                     /locus_tag="AC16CX_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR3014244.1"
                     /translation="MNLPKRVKLVEVGPRDGLQNEPTPVATEVKVELIHRLQDAGLKV
                     IEATAFVSPRWVPQMADNAQVMAAIRRKPGVSYPVLVPNRKGLDAALAARAEEVVVFG
                     AATETFSRKNTNCSIAEGLARFSEVCREAIARSLQVRGDISVCLGCPYEGEVAPDAVV
                     RVARELDAMGCYEITICDTIG"
     gene            complement(791..1642)
                     /locus_tag="AC16CX_17020"
     CDS             complement(791..1642)
                     /locus_tag="AC16CX_17020"
                     /inference="COORDINATES: protein motif:HMM:NF027439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3014245.1"
                     /translation="MSQKVLSQVAADGNATVMLNRPEVHNAFDPEMVGALTATLKKLE
                     SNDRVRAIVITGAGRNFCAGADIEHMKKSARFSREQNLDNARATAQMLWTLYALEKPT
                     IACVRGAARGGGVGLIAACDIAIAERTATFRLSEVKLGIVPAMISPYVVAAIGQRHSH
                     RYMLSGEEFDSAEAFRIGLVHDICEEPELNATVGRMLAHLYSSGPNAVVAIKKMIPEV
                     ASTRVGEALVEMTAQRIAEIRTTAEAQEGLSAFLEKRKASWVTPASEKSKSKANRHAA
                     KKSKDRN"
     gene            complement(1771..>2165)
                     /locus_tag="AC16CX_17025"
     CDS             complement(1771..>2165)
                     /locus_tag="AC16CX_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3014246.1"
                     /translation="VPKFTVIIGGSFGAGNYGMCGRAYSPRFLWTWPNARISVMGGEQ
                     AANVLAQVKREALEKGGKEWSRKEEEAFKAQIVEQYTRQSSAYYASARLWDDGIIDPK
                     ETRQTLALGISASLNAPIQETRFGVFRM"
BASE COUNT          371 a          712 c          649 g          433 t
ORIGIN      
        1 tgccgatggt gtcgcagatg gtgatctcat agcagcccat cgcgtcgagc tcgcgcgcga
       61 cccggaccac cgcgtcgggc gcgacctcgc cttcgtacgg gcagccgagg cacaccgaga
      121 tgtcgccacg cacctggagg ctccgagcga ttgcctccct gcacacctcc gaaaagcgcg
      181 cgagaccctc cgcgatcgag cagttggtgt ttttccgcga gaaggtttcc gtcgccgcgc
      241 cgaacaccac gacctcttcg gctcttgcgg cgagcgcggc gtcgagcccc ttcctgttcg
      301 gcacgaggac cgggtaggac acgcccggct tgcggcggat cgccgccatc acctgcgcgt
      361 tgtccgccat ctgcggcacc cacctgggcg agacgaaggc ggtcgcctcg atcaccttca
      421 accctgcgtc ctgcaggcgg tggatgagct cgaccttgac ctcggtagcg accggcgtgg
      481 gctcgttctg cagcccgtcg cgcggcccga cttcaaccaa cttcactcgt ttcggcaaat
      541 tcatttcacc gccaagattt caagaatgaa atcaaaaccc agatcgaacc gccaagacgc
      601 caagagaaac aagtacaaaa tagaaccgaa aatcaaaccc cattgaaccg ctgagataca
      661 gagagaatca aatcattgac ggaaataaaa gtccaacaaa aagtttttcg caaaaaagta
      721 cctgtatttg cttctctctt tgtctccgtg gttcagttat cgctccggtt ttcttggcgg
      781 ccttggcggt tcaattcctg tccttgcttt tcttggcggc gtggcgattt gcttttgatt
      841 ttgacttctc ggaggcgggg gtgacccacg acgccttacg tttttccagg aacgcggaca
      901 ggccttcctg cgcctccgcg gtggtgcgga tctccgcgat gcgctgcgcg gtcatttcca
      961 cgagcgcttc gcctacgcgg gtgctggcca cttcggggat catctttttg atcgcgacca
     1021 ccgcgttggg gccgctcgaa tagagatgcg ccagcatgcg gccgacggtg gcgttcaact
     1081 ccggctcctc gcagatgtcg tgcaccagcc cgatgcggaa cgcctcggcg gaatcgaact
     1141 cctcgcccga gagcatgtag cggtgcgagt ggcgctgccc gatcgccgcg accacatagg
     1201 ggctgatcat ggcgggcacg atgccgagct tcacctccga gaggcggaac gtggcggtgc
     1261 gctcggcgat cgcgatgtcg caggcggcga tcagccccac gccgccgccg cgcgccgcgc
     1321 cgcgcacgca ggcgatggtc ggcttctcga gcgcgtagag cgtccacagc atctgcgcag
     1381 tggcgcgcgc gttgtcgagg ttctgctcgc gcgagaaccg ggcgctcttc ttcatgtgct
     1441 cgatgtcggc accggcgcag aaattgcggc ccgcgccggt gatcacgatc gcgcgcaccc
     1501 tgtcgttcga ttcgagcttt ttcagcgtcg ccgtcagcgc acccaccatc tcgggatcga
     1561 atgcgttgtg cacctccggc cggttcagca tcaccgtcgc attgccgtcg gcagccactt
     1621 gcgagaggac tttctggctc atctgaattc cggggcggga aaggcgctca ttatagcttc
     1681 gtcattggaa agccgtggat cgtggatcgt gattcgtcgc attctccccc gtcctggctt
     1741 cccaaccgaa tcgacccacg acccacggga ttacatgcgg aagacgccga agcgcgtctc
     1801 ctggatcggg gcgttgaggc tggcggagat gccgagggcg agtgtctgcc gcgtctcctt
     1861 cgggtcgatg atgccgtcgt cccacagccg cgcgctggcg tagtaggcgc tcgactggcg
     1921 cgtgtactgc tcgacgatct gcgccttgaa cgcctcctct tccttcctgc tccattcctt
     1981 gccgcctttc tccagcgctt cgcgcttgac ctgcgcgagc acgttcgccg cctgctcgcc
     2041 gcccatcacc gagatcctgg cgttgggcca cgtccagaga aagcgcggcg aataggcgcg
     2101 accgcacatg ccgtagttgc cggcgccgaa actgccgccg atgatgacgg tgaacttggg
     2161 aacgc
//
LOCUS       DBNGWZ010000548         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3225633, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000548 DBNGWZ010000000
VERSION     DBNGWZ010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3225633"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16CX_17030"
     CDS             complement(<1..638)
                     /locus_tag="AC16CX_17030"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014247.1"
                     /translation="MADYAIKEQTSRLPKEVIHHAKRAVVDWYASLLPGSRVPPATLL
                     EQALAEELDHGRARLASGRRTPLRVAALINGAASHSVEFDDIWRDAVYHPASPVISAA
                     LAAAQTLGANGERFLRGVIVGYEVSTRIGDAVMPSHYRFWHTTGTVGTFGAAAAVATL
                     LGCNREQFVHTLGNAGTFAAGLQQAFRSQAMAKPLHGGHAAEAGALAALCAL"
     gene            complement(760..>2105)
                     /locus_tag="AC16CX_17035"
     CDS             complement(760..>2105)
                     /locus_tag="AC16CX_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3014248.1"
                     /translation="VYKLGRSEVGVELATSHTGAMTGSDDTAGAFFRAHGMLRVEMLE
                     TLFELPALILGQRPAARHRVAAMTTTGGGAATVVDRLGTLGVEVVPPTDRIVANLERR
                     KISITRARLTDLTLAGAKKEIYSAVLNELLASDHCDLVLAVVGSSAQFQPHIAIDPIL
                     ESARGAKPLAVFCAPQADASLALLSKAGIAGFRTPEACADAIRAWRDWCAPLPPPAPD
                     ATRIGSARALVGKERRLNEQMACRVFDALGVPAAPSEVITSPEQAVKLGFPVVAKILS
                     PDIAHKTDAGGVAIGITTPEELKRAAAAILQRVRAKHPGARVSGVLVQRMEKGLAEVI
                     LGYKRDPQVGPVVVLGVGGVLAEIYRDFAVRLAPVGAEEAANMIEEVRGLAVIRGYRG
                     LPRGDTAALARAVAAFSQLAHLEPRVAEAEINPLIVRPEGEGVVAVDGLLVLADN"
BASE COUNT          328 a          750 c          699 g          328 t
ORIGIN      
        1 tgcagggcgc acagcgcggc gagcgcaccg gcctccgccg cgtgcccgcc gtgcagcggc
       61 tttgccatcg cttgcgatcg gaacgcctgt tgcagccccg cggcgaaagt gccggcgttg
      121 ccgagcgtgt gcacgaactg ctcgcggttg cagccgagta gggtggcgac ggcggcggcg
      181 gcgccgaacg tgccgacggt gccggtcgtg tgccagaacc tgtaatggga cggcatcacg
      241 gcgtcaccga tgcgcgtgga aacctcgtag cccacgatga cgccgcgcag gaaccgctcg
      301 ccgttcgcgc ccagggtctg cgccgcggcg agcgccgcgg aaatcaccgg gctcgcgggg
      361 tgatagaccg cgtcacgcca gatgtcgtcg aactccacgg aatgcgacgc cgcgccgttg
      421 atgagcgcgg cgacccgcag cggcgtccgg cgtcccgaag ccagccgcgc ccgtccgtga
      481 tccagctcct cggcgagcgc ctgctcaagc agagtggccg gcgggacgcg actgccgggc
      541 agcagtgaag cgtaccagtc caccaccgcg cgcttggcgt ggtggatcac ctctttcggc
      601 agccgcgagg tctgttcttt gatagcgtag tcagccatct gtctcagtag catcggcttc
      661 tcctacctga acttcttcga cgcaaagtcg ctaagccgca aagttatcgg ggataaaaat
      721 ttgaaacctt tgcgcctttg cgtcaaggtt ttcgctcggt taattgtccg cgaggacgag
      781 caggccgtcc acggccacca cgccctcgcc ctcgggtctg acgatcagcg ggttgatctc
      841 cgcctccgcg acgcgcggct cgaggtgtgc gagctgcgag aaggcggcga ccgcccgcgc
      901 cagcgccgcg gtatcgccgc ggggcaaccc gcggtagccg cggattacgg cgagccccct
      961 cacctcctcg atcatgtttg ccgcttcctc cgcccccacc ggggcaagcc gcaccgcgaa
     1021 atcccgatag atttcagcaa gcaccccgcc cacgcccagc accaccacgg gaccgacctg
     1081 cgggtcgcgc ttgtagccca gtatcacctc cgccaagccc ttctccatgc gctgaaccag
     1141 cacgccgctg actcttgctc ccgggtgctt cgcccgcacg cgctgcagga tcgccgccgc
     1201 cgcccgtttc agttcctcgg gcgtggtgat gccgatcgcc acgcctccgg cgtcggtctt
     1261 gtgagcgatg tccggagaaa gaatcttcgc caccaccggg aaaccgagct tcaccgcctg
     1321 ctcgggagat gtgatcacct ccgagggcgc ggccggcacg cccagggcgt cgaacaccct
     1381 gcacgccatc tgctcgttca atcgccgctc ttttccgacc agcgcgcggg cggacccgat
     1441 gcgcgtggcg tcgggcgcag gcggcggcaa cggcgcgcac cagtcgcgcc aggcgcggat
     1501 cgcgtccgcg cacgcctcgg gggtgcggaa gccggcgatg ccggctttgc tcaatagcgc
     1561 gagggaagca tcagcctgcg gcgcgcagaa taccgccagc ggcttggctc cacgcgcgga
     1621 ttccaggatc gggtcgatcg cgatatgcgg ctggaactgc gccgagctcc ccaccaccgc
     1681 tagcaccagg tcgcaatgat cggaggcgag cagctcgttc agcaccgcgc tgtagatctc
     1741 cttcttcgcg ccggcgagcg tgagatcggt cagccgcgcg cgcgtgatgc tgattttcct
     1801 gcgttcgaga ttggcgacga tgcggtcggt gggtggcacc acctcgacac cgagcgtgcc
     1861 gaggcggtcc accaccgtcg ccgcgccgcc gccggtggtc gtcatggccg ccacccggtg
     1921 gcgggcggcc ggccgctggc cgaggatcag cgccggcagc tcgaacagcg tctcgagcat
     1981 ctccacccgc agcatgccat gggcgcgaaa aaaagcgccc gccgtgtcgt cggagccggt
     2041 catcgcgccg gtgtgcgagg tggcgagctc gacaccgacc tcggagcggc cgagcttgta
     2101 gacga
//
LOCUS       DBNGWZ010000549         6311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_329130, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000549 DBNGWZ010000000
VERSION     DBNGWZ010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_329130"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="AC16CX_17040"
     CDS             complement(<1..148)
                     /locus_tag="AC16CX_17040"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877691.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3014249.1"
                     /translation="MKKVGFIGWRGMVGSVLMERMRAERDFELIEPVFFSTSRMGAKG
                     PEIGK"
     gene            complement(282..1352)
                     /gene="leuB"
                     /locus_tag="AC16CX_17045"
     CDS             complement(282..1352)
                     /gene="leuB"
                     /locus_tag="AC16CX_17045"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3014250.1"
                     /translation="MKIAVLAGDGIGPEIVAQAVKALKALACDGLGLELEEAPFGGAG
                     VDAHGDPLPEPTLKLARAADAVLCGAVGGPKYDALPRPQRPEQGILRIRKELGLFANL
                     RPALMFPELVGASSLKAEVVSGLDIMIVRELTGDIYFGEPRGRRSNARGEREGYDTMH
                     YSEPEVRRIAEVGFATAMKRNRRLCSVDKENVLETSRLWREVVGDVGKRYPQVELTHM
                     YVDNAAMQLVRAPRHFDVIVTGNLFGDILSDEASMLTGSIGMLPSASLDADSKGLYEP
                     VHGSAPDIAGKNLANPLATILSSAMMLRYTFNQEEAAMRIEQAVRKVLVQGYRTVDIF
                     EPGTRKVGTKEMGDAVAVALQS"
     gene            complement(1450..2088)
                     /gene="leuD"
                     /locus_tag="AC16CX_17050"
     CDS             complement(1450..2088)
                     /gene="leuD"
                     /locus_tag="AC16CX_17050"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3014251.1"
                     /translation="MDKFVRKEGLVAPMDRANVDTDAIIPKQYLKSIKRTGYGPNLFD
                     EWRYLDHGEPGMDHSRRPLNPDFVLNQPRYRGATVLVARENFGCGSSREHAPWALLQY
                     GFQVVIAPSYADIFYNNSLKNGLLLIRLDANVVDKLFRETFATEGYRLAVDLESQTVT
                     TPGGEHFKFDIDPFAKHCLLNGLDEIGLTLRRAGKIRAFEAKHRERQPWLFT"
     gene            complement(2090..3505)
                     /gene="leuC"
                     /locus_tag="AC16CX_17055"
     CDS             complement(2090..3505)
                     /gene="leuC"
                     /locus_tag="AC16CX_17055"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3014252.1"
                     /translation="MTARTLYDKLWDGHLVHQEPDGTALIYIDRHLVHEVTSPQAYEG
                     LKLAGRKPWRVDSIVATADHNTPTRNWDQGIQGIADPVSRLQIETLDANIKEFGAKVY
                     FPFLDRQQGIVHVIGPEQGATLPGMTLVCGDSHTSTHGALGCLAFGIGTSEVEHVLAT
                     QCMMMKKPKAMQIVVEGRLGRGVTAKDIILAIIGKIGSAGGTGYAIEYAGNTIRALSM
                     EGRMTICNMSIEAGARAGMVAVDDATIEYVKGRPLAPKGAMWDKAVAYWRTLVSDPGA
                     KFDKVVKLDAAEIQPQVTWGTNPQMVTTIDGKVPDPKDEKDPVKREWMERALKYMDLK
                     PNTPITEIRPDKVFIGACTNSRIEDLRAAAQVVKGKRVASSIKLALVVPGSGLVKQQA
                     EREGLDRIFTEAGFEWRQPGCSMCLGMNDDQLSPGERCASTSNRNFEGRQGPGGRTHL
                     VSPVMAAAAAITGHFVDVRKF"
     gene            complement(3645..4793)
                     /locus_tag="AC16CX_17060"
     CDS             complement(3645..4793)
                     /locus_tag="AC16CX_17060"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014253.1"
                     /translation="MAALPYRQLAGFYFFYFAYIGAFAPFFSIYLDAVGLSPVEIGAV
                     MALPQVTRIVAPHLWGWLADAGDRQVRLVRVTALAGTLCWLGMFASTAFLWICAVILA
                     VGFFLSAALPLVEATTLTHLGEQTGGYGVIRLWGSIGYIVAVVGMGYALDFLAVSALL
                     WIVAASLAGTLAFAWLIPDAAQVSHGSDQQPIAHILRRPEVIALISACTLMAVAHGPY
                     YTFYSIHLVDHGYSKGLTGWLWALGVVCEIAIFLWLPRLYAAFTLRQTLIASFALAGV
                     RFLLIGWGAESLVLLLLAQTLHAASFGSFHAAAIGVVHQVFRGRHQARGQAIYGSLTF
                     GVGGTVGSLASGYAWEPLGAALTFTAAAAAALAGMLLLWWKLKVGNGS"
     gene            complement(4804..5967)
                     /gene="aroC"
                     /locus_tag="AC16CX_17065"
     CDS             complement(4804..5967)
                     /gene="aroC"
                     /locus_tag="AC16CX_17065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3014254.1"
                     /translation="MSGSTLGKLFCVTSFGESHGPAIGCVVDGCPPGLELSEVDIQPE
                     LDRRKPGTSRHVTQRREEDTVEILSGVFEGRTTGTPIALLIRNVDARSKDYSKIKDMF
                     RPGHADYTYLQKYGIRDYRGGGRQSARETAVRVAAAAIARKWLREKYGVAVRGYMAQL
                     GPIEIPFKTWDAVNTNPFFAPNAEIVPQLEEFMDKLRKSGDSCGARITTVASGVPVGW
                     GEPVYDKLDADLAYNMMSINAVKGVEIGAGFRAVAQKGTEHSDEMSPQGFLSNNAGGI
                     LGGISTGQDIVVSVAVKPTSSIRLPRRTIDRAGKPTVIETHGRHDPCVGIRATPICEA
                     MMALTLMDHALRHRAQNADVACETPRIPAQAPADVIERLRAAAAVENPDPDEA"
     gene            6116..6310
                     /locus_tag="AC16CX_17070"
     CDS             6116..6310
                     /locus_tag="AC16CX_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014255.1"
                     /translation="MADKPDPGKMKGPGGLTMHELHELVKKSHARDQAASKVHNKLAQ
                     PQSRWQRFVRYVWDKNRGRG"
BASE COUNT         1105 a         2126 c         1960 g         1120 t
ORIGIN      
        1 ttttgccgat ctccggtcct ttggcaccca tccgtgaggt ggagaaaaac accggctcga
       61 tcagctcgaa gtcgcgttcc gcgcgcatgc gctccatcag cacggagccc accatgccgc
      121 gccagccgat aaaacccact ttcttcatcg caaccgcctg ggatctggaa tggctctatt
      181 ctatcaagcc attgagccgt aatgggtaat gggttatgag cgataggcaa caaaacaaca
      241 gcgagaacca ctcgtacccc ttactcatga cccgtcaccc attacgattg gagagcaacg
      301 gcgacggcat cgcccatttc cttcgtgccg accttccgcg tgccgggttc gaagatgtct
      361 accgtgcgat agccctggac gagcactttc ctcaccgcct gctcgatgcg catcgccgct
      421 tcctcttgat tgaacgtgta gcgcagcatc atcgccgacg agagtatcgt tgcgagcggg
      481 ttcgccaggt tctttcccgc gatgtcgggg gcggagccgt gcaccggctc gtagagcccc
      541 ttcgagtccg cgtcgagcga agccgagggc agcatcccga tcgagccggt cagcatcgag
      601 gcctcgtccg acaggatgtc gccgaacagg ttgccggtga cgatgacatc gaagtgcctg
      661 ggcgcccgca cgagctgcat cgcggcattg tccacgtaca tgtgggtcag ctcgacctgc
      721 ggatatcttt tcccgacgtc gcccaccact tcccgccaca gccggctcgt ctcgagcacg
      781 ttctccttgt ccaccgagca cagcctgcgg ttgcgcttca tcgccgttgc gaacccgact
      841 tcggcgatgc ggcggacctc gggctccgag tagtgcatgg tgtcgtagcc ctcgcgctct
      901 ccgcgcgcgt tggagcgacg tccgcgcggt tcgccgaaat aaatgtcgcc ggtgagctcc
      961 cgcacgatca tgatgtcgag ccccgaaacc acctccgcct tcaggctcga tgcgccaacc
     1021 agctcgggga acatcagggc cgggcgcagg ttggcgaaca gccccagctc cttgcgtatg
     1081 cgaaggatcc cctgctccgg ccgctgtggc cgcggcagcg cgtcatactt cggaccgccc
     1141 accgcgccgc acagcaccgc gtccgcagcg cgcgcgagct tgagcgtggg ttcgggcagc
     1201 ggatcgccgt gcgcgtccac gccggcgccg ccaaacggcg cctcctcgag ttcgagtccg
     1261 agcccgtcgc acgccagtgc tttcagcgcc ttgactgcct gcgcgacgat ctccggcccg
     1321 atgccgtcac cggccaatac cgcaatcttc atccttcgga ttccgatttg gtgagggtga
     1381 ggagagaggt gtaaggagca ggatcacggg gatgttctta cgcctcaccc ctcacccttc
     1441 acacctcact taagtgaaca gccagggctg ccgctcgcga tgcttcgcct cgaacgccct
     1501 gatcttgccg gcgcgccgca gcgtgagccc gatctcgtcc agcccgttca acaggcagtg
     1561 cttcgcgaac ggatcgatgt cgaacttgaa gtgttctccg ccgggcgtcg tcacggtctg
     1621 gctctcgagg tccaccgcga gccggtagcc ctcggtcgcg aaagtctcgc ggaacaattt
     1681 gtccacgacg ttcgcgtcga gcctgatcag gagcagcccg ttcttgagac tgttgttgta
     1741 gaagatgtcg gcgtagctcg gcgcgatcac cacctggaag ccgtactgca gcagcgccca
     1801 cggcgcgtgc tcgcgcgagg agccgcagcc gaagttctcg cgggcaacca gcacggtcgc
     1861 gccccggtag cgtggctggt tcagcacgaa atccgggttg agcggtcgcc tggaatggtc
     1921 catgccgggc tcgccgtggt cgagatagcg ccactcgtcg aagaggttcg ggccgtaacc
     1981 ggtgcgcttg atggatttga ggtactgctt cgggatgatg gcgtcggtgt ccacgttggc
     2041 gcggtccatc ggcgccacca gaccctcctt cctcacaaac ttgtccatgt caaaatttcc
     2101 gcacgtccac gaaatgccct gtgatcgccg ccgctgcggc catgaccgga ctcacgaggt
     2161 gagtccttcc gccagggccc tgcctgcctt cgaagttgcg gttcgaggtc gatgcgcaac
     2221 gctcgccggg cgagagctga tcgtcgttca tgcccaggca catcgagcag cccggctggc
     2281 gccattcgaa gccggcctcg gtgaaaatcc tgtcgagccc ctcccgctcg gcctgctgct
     2341 tcaccaggcc ggagccgggc acgaccagcg ccagcttgat gctggaggcg acgcgcttgc
     2401 ccttgaccac ctgcgccgcc gcgcgcaggt cctcgatgcg cgagttggtg caggcgccga
     2461 tgaacacctt gtcgggcctg atctcggtga tcggcgtgtt gggcttcagg tccatgtact
     2521 tgagcgcgcg ctccatccac tcgcgcttga ccgggtcttt ctcgtccttc gggtccggca
     2581 ccttgccgtc gatcgtggtc accatttgcg gattggtgcc ccaggtcacc tgcggctgga
     2641 tctccgccgc gtcgagcttc accaccttgt cgaacttcgc gccgggatca ctcacgaggg
     2701 tgcgccagta cgcaaccgcc ttgtcccaca tcgcgccctt gggcgcgagc ggccggccct
     2761 tcacgtactc gatcgtggcg tcgtccacgg ccaccatgcc ggcgcgcgcg cccgcctcga
     2821 tcgacatgtt gcagatggtc atgcggcctt ccatcgaaag cgcgcggatg gtgttgcccg
     2881 cgtactcgat cgcgtagccg gttccgcccg cgctgccgat cttgccgatg atcgcgagga
     2941 tgatgtcctt cgcggtcacg cctcgcccga gccggccctc aaccacgatc tgcatggctt
     3001 tcggcttctt catcatcatg cactgggtcg cgagcacgtg ctcgacctcg ctcgtgccga
     3061 tgccgaaggc gaggcagccg agcgcgccgt gcgtgctggt gtgcgagtcg ccgcacacga
     3121 gcgtcatccc gggcagcgtc gcgccctgct ccgggccaat cacgtgtact atgccctgct
     3181 gcctgtcgag gaacgggaaa taaaccttcg ccccgaattc cttgatgttc gcatccaggg
     3241 tttcgatctg cagccgtgaa accgggtccg cgataccctg tatcccttgg tcccagttgc
     3301 gggtcggcgt gttgtgatcg gcggtcgcga cgatggaatc cacgcgccac ggcttgcgac
     3361 cggcgagctt cagcccctcg tacgcctgcg ggctcgtcac ttcatgcacc aggtgacggt
     3421 cgatgtagat caacgccgtg ccgtcgggct cctggtgaac gaggtggccg tcccacaatt
     3481 tgtcgtagag cgtgcgcgcc gtcatgaatt acgtaacctc atggaaacgc tggattatta
     3541 cacatccagc aaggccagaa cagattaaac ccgtgaatcg tgatttgtga atgtgattgg
     3601 taatgcaggc cgttattgct gagacaactt catcgataca cgattcacga cccattgccc
     3661 accttcaatt tccaccacag caacagcatt cctgcgagcg ccgcggcagc ggcggccgta
     3721 aatgtcagag ccgcgccgag cggctcccac gcatagccgc tcgcgaggct gcccacggtg
     3781 ccgccgacgc cgaaggtcag gctgccgtaa atcgcctggc cgcgcgcctg gtggcggccg
     3841 cgaaacacct ggtgcacgac cccgatcgcc gcggcgtgaa aagagccgaa gctcgccgcg
     3901 tgcagcgtct gcgcgagcag cagcaggacg aggctttccg ccccccagcc gatgagaaga
     3961 aaacgcaccc ccgccagcgc gaagctcgcg atcagggtct ggcgcagcgt gaaagccgcg
     4021 tacagacgcg gaagccacag gaagatcgcg atctcgcaga ccacgccgag cgcccatagc
     4081 cagccggtca gccccttgct gtagccgtgg tccacgagat ggatcgagta gaacgtgtaa
     4141 tacgggccgt gcgccaccgc catcagcgtg caggccgaga tcagcgcgat cacctcggga
     4201 cgcctcagaa tgtgcgcgat cggctgctga tcggagccgt gggatacctg cgctgcatcg
     4261 ggaatcaacc aggcgaatgc cagcgtcccc gcgagcgagg cagccacgat ccagagcagc
     4321 gcgctcaccg cgagaaaatc gagcgcgtag cccatgccga ccacagccac gatgtagccg
     4381 atcgagcccc acagccggat cacgccgtaa ccgccggtct gctcgcctag atgggtcagc
     4441 gtcgtcgcct cgaccagcgg cagcgcggcg ctcaggaaaa acccgaccgc caggatcacc
     4501 gcgcagatcc agagaaacgc ggtggacgcg aacatgccga gccagcacag cgtccccgcc
     4561 agcgcggtga cgcgcaccag gcgcacctgc cggtcgccag catcggcgag ccagccccat
     4621 aggtgcggcg cgacgatacg ggtgacctgc ggcagcgcca tcaccgcgcc gatttccacc
     4681 ggcgagaggc cgaccgcgtc gagatagatg ctgaagaacg gcgcgaacgc gccgatgtac
     4741 gcgaaataga aaaagtagaa gccggcgagt tgccggtacg gcagagctgc catggggagg
     4801 cggctacgcc tcgtcgggat cggggttctc gaccgccgca gccgcgcgca gcctctcgat
     4861 cacgtccgcc ggcgcctgcg ccgggatgcg cggcgtctcg cacgcgacgt cggcgttctg
     4921 ggcgcggtgg cgcagcgcat ggtccatcag cgtcagcgcc atcatcgcct cgcagatcgg
     4981 cgtcgcgcgt atgcccacgc acgggtcgtg gcggccgtgg gtctcgatca cggtcggctt
     5041 gcccgcccgg tcaatcgtgc gccgcggcag gcgtatgctg gaagtgggct tcaccgcgac
     5101 gctcacaacg atgtcctggc cggtggagat gccgccgagt atgccgcccg cgttgttcga
     5161 gagaaacccc tgcggcgaca tctcgtcgga atgctcggtg cccttttgcg cgaccgcgcg
     5221 gaagcccgcg ccgatctcca cgcccttcac ggcattgatg ctcatcatgt tgtaggcgag
     5281 gtccgcgtcg agcttgtcat acaccggctc gccccagccg accggtacgc cggatgcgac
     5341 cgtcgtgatg cgcgccccgc acgaatctcc cgatttgcgc agcttgtcca tgaactcctc
     5401 gagctgcggc acgatctccg cgttgggcgc gaagaacggg ttggtattga ccgcgtccca
     5461 ggtcttgaac ggaatctcga tcgggccgag ctgcgccatg tagccacgga ccgcgacgcc
     5521 atacttttcg cgcagccact ttcgcgcgat agcggcggcg gccacccgta ccgcggtttc
     5581 gcgcgccgat tgccgtccgc cgccgcggta atcgcggatg ccgtacttct gcagataggt
     5641 gtaatcagcg tgccccggcc ggaacatgtc cttgatcttc gagtagtcct tgctccgcgc
     5701 atccacgttg cggatgagaa gcgcgatcgg ggtgccggtg gtcctgccct cgaatacgcc
     5761 agagaggatc tcgacggtgt cctcttcgcg ccgctgcgtg acatggcgcg aagtgccggg
     5821 cttgcggcgg tcgagctcag gctgtatgtc cacttcggag agttccagtc cgggcgggca
     5881 gccgtccacc acgcagccga tcgccggccc gtggctctcc ccgaaggagg tcacgcaaaa
     5941 gagtttccca agcgtgctgc cggacatcgt tctaagtggc tgttgccggt ttggcagcaa
     6001 cagcccctga tgcaggggtg cacgagggga gtatccccac ctgcgttacg gccaaaactc
     6061 gaaaaaattc aaggacgagt ttaacatatc gagtactcac cactggcgct gcgcgatggc
     6121 ggacaagccc gatcccggca agatgaaagg ccccggcggc ctgacgatgc acgagctgca
     6181 cgaactcgtg aagaagagcc atgcgcgcga ccaggcggcg agcaaggtgc acaacaagct
     6241 cgcgcagccg cagagccgct ggcagcgctt cgtgcgctac gtgtgggaca aaaatcgtgg
     6301 ccgaggctaa g
//
LOCUS       DBNGWZ010000550         3156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2219641, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000550 DBNGWZ010000000
VERSION     DBNGWZ010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2219641"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16CX_17075"
     CDS             <1..525
                     /locus_tag="AC16CX_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly lipoprotein LptE"
                     /protein_id="HIR3014256.1"
                     /translation="LRRFAQRLSFRLSPSAFLLAATALLVAACGFQLRGTVSLPFNSL
                     YVQAAPTSQLATQLKRAVRSGSGTRIAETPEQAEVILHIMNERQEKQILSLGGGGRVR
                     EFQLRYRVSFRLTDRENREHIPASEILMQRDFTFNDDAVLSKEAEEALLYRDMRNDAV
                     QQLVRRLQAAKLQS"
     gene            684..1772
                     /gene="holA"
                     /locus_tag="AC16CX_17080"
     CDS             684..1772
                     /gene="holA"
                     /locus_tag="AC16CX_17080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3014257.1"
                     /translation="MRITTEQLQQHLARELKPLYTVFGDETLLALEASDRIRAKAWAE
                     GYTERNVLTADSGFKWNELAFTGNSQSLFATRRILELRIPTGKPGAEGSETLQSYCEG
                     LPPDTVTLVCLPGIDWRAQKSGWFEALDRAGATIEAKPVARKALPEWLAGRLRAQNQE
                     ADGETLDFIADRVEGNLLAAHQEVQKLALLFPAGRIAYEQVREAVLDVARYDVFNLGE
                     ALLEGDVLYLARMMDGLRGEGAAPPLVLWAVAEEIRAIGRVLDGMASGKTPPQLWRDT
                     KVWGNSHQGLMQQTCRRFTKEQVEAALLHAAKVDRMVKGLIRGDVWDELLQLCLRFAS
                     SAPAKSLPKQGKMAAAARAAERSQPGLF"
     gene            1907..>3156
                     /locus_tag="AC16CX_17085"
     CDS             1907..>3156
                     /locus_tag="AC16CX_17085"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR3014258.1"
                     /translation="MDVGTYIHNVGRQARAAARVIARADTATKNRALREMATAIERNR
                     GRLLEANARDLEEARKQHLDSAMIDRLTLTPKVITAMADGLRQIAALPDPVGEITELR
                     KRPSGIKVGKMRVPLGVVAIIYESRPNVTADAAGLCIKSGNAAILRGGSEAIHSNQAI
                     AACVHAGLRAAGLPETVVQVIETTDRAAVGELITMQDTVDVIVPRGGKSLIARLMRES
                     RIPMIKHLHGVCHTYIDDKADLRKAIRIADNAKTQRLGTCNTMETLLVARGIAQKVLP
                     PLARVYLEKGIELRGDDEARKIVPEMKPATEEDWYTEYLDAILSVRVVKDLDEAMDHI
                     ATYGSQHTDAIVTEDKARARRFLREVDSSSVMVNASTRFADGYEYGLGAEVGISTDKL
                     HARGPVGLEGLTSLKWVVFGTGQI"
BASE COUNT          580 a         1052 c         1041 g          483 t
ORIGIN      
        1 ctcaggcggt ttgcacaacg cctttccttt cgcctttcgc cttccgcctt tctccttgcc
       61 gcgaccgcat tgctggtcgc ggcgtgcggc ttccagcttc gcgggacggt gtcactgccc
      121 ttcaactcgc tgtacgtgca agcagcgccc acctcccagc ttgcgaccca gcttaagcgc
      181 gcggtgcgct caggcagcgg cacgcgcatt gccgagactc cggaacaggc cgaggtgatc
      241 ctgcacatca tgaatgagcg gcaggaaaag cagatcctct cgctgggcgg cggcgggcgc
      301 gtccgcgaat tccagttgcg ctaccgcgtt tccttccgcc tcaccgaccg cgagaaccgc
      361 gagcacatcc cggcgagcga gatcctgatg caacgcgact tcaccttcaa cgacgacgcg
      421 gtgctgtcga aagaggcgga ggaagcgctg ctctaccgcg acatgcgcaa cgacgcggtg
      481 cagcagctgg tgcggcgctt gcaagcagca aagttgcagt cctagacgca aaggcgcaaa
      541 gaaagcactt ttcgtcagct agagcggctt cggggctatg cgattcttgg cgcatcgggc
      601 gggtggccgc aagcgatggc ctctgccatc gtgtttacta tagaatctct ttgcggcttt
      661 gcgccgttgc gtccaataaa ctcgtgcgaa taacaaccga gcagctgcaa cagcacctcg
      721 cccgggagct gaagcccctc tacaccgtat tcggcgacga gacgctgctt gcgctggagg
      781 catccgaccg catccgcgcc aaggcctggg ccgagggtta caccgagcgc aacgttctca
      841 cggcggattc cggcttcaag tggaacgagc tcgccttcac cggcaactcg caatccctgt
      901 tcgccacccg ccgcatcctc gaactgcgca tccccaccgg caagccggga gcggaaggat
      961 cggaaacgct gcaaagctac tgcgaagggc tgccgcccga caccgtcacg ctcgtctgcc
     1021 tcccgggaat cgactggcgg gcgcaaaaat ccggctggtt cgaggcgctc gaccgcgccg
     1081 gggccacgat cgaggcaaag ccggtcgcgc gcaaggcgct gcccgaatgg ctcgccgggc
     1141 ggctccgcgc ccagaaccag gaggcggacg gggaaacgct ggatttcatc gccgaccgcg
     1201 tcgagggcaa tctcctcgcg gcgcatcagg aagtgcagaa gctcgctctg ctgttccccg
     1261 ccggcaggat cgcctacgag caggtgcgcg aagcggtgct cgacgtcgcg cgctatgacg
     1321 tgttcaacct cggcgaggcg ctgctcgagg gcgacgtgct gtaccttgcg cgcatgatgg
     1381 acgggttgag gggcgaaggc gccgcgcctc cgctcgtgct gtgggcggtc gccgaggaga
     1441 tccgtgccat cgggcgcgtg ctcgacggca tggcctctgg caagacgccg ccgcagttgt
     1501 ggcgcgacac caaggtatgg ggcaactccc atcagggcct catgcagcaa acctgccggc
     1561 gcttcacgaa ggaacaggtc gaggcggcgt tgctgcacgc ggcaaaggtg gaccgcatgg
     1621 tgaagggcct gatccgcggc gacgtgtggg acgagctgct ccagctgtgc ctgcgcttcg
     1681 cctccagcgc gcccgccaag tccttgccga aacagggtaa aatggccgct gccgcgcgcg
     1741 cagcggaacg cagtcaaccg ggactgttct agttcctgtt aaaccggaag cgcagctccg
     1801 atttaactcc gcgcgttcga acggacgacc aggactcggc ggtcgcgatc cccgcgccct
     1861 aagtcgccgc tcaacgcgcg acgtgccgcc cgcggcggga ttttccatgg acgtcggaac
     1921 ctacatacac aacgtcggcc gccaggcgcg cgcagctgcg cgcgtcatcg cccgcgccga
     1981 taccgcgacc aagaaccgcg cgcttcggga gatggctacg gcaatcgagc gcaaccgcgg
     2041 gcggctgctc gaggccaacg cgcgcgatct cgaggaagcc aggaagcagc atctggattc
     2101 ggcgatgatc gaccggctga cgctcactcc caaggtgatc acggcgatgg cggacggcct
     2161 gcggcagatc gcggcgctgc ccgatccggt gggcgagatc accgagctca ggaagcgccc
     2221 ctcggggatc aaggtgggca agatgcgcgt gccgctcggc gtggtcgcca tcatttacga
     2281 gtcccgcccc aacgtcaccg ccgacgcggc gggcctgtgc atcaagtccg gcaacgcggc
     2341 gatcctgcgc ggcggctcgg aagcgatcca ctccaaccag gcgatcgccg cttgcgtgca
     2401 cgccggactc cgggccgcgg ggctgccgga gaccgtggtg caagtgatcg aaaccaccga
     2461 ccgcgcggcg gtgggcgaat taataacaat gcaggacacc gtggacgtga tcgtgccgcg
     2521 cgggggcaag agcctgatcg cgcggctgat gcgcgaatcc cgcatcccga tgatcaagca
     2581 cctgcacggc gtgtgccata cctacatcga cgacaaggcc gacctgagga aggcgatccg
     2641 catcgccgac aacgccaaga cgcagcggct cggcacctgc aacacgatgg agacgctgct
     2701 ggtcgcgcgc ggcatcgccc aaaaggtcct gccgccgctc gccagggtct atctcgagaa
     2761 aggcatcgag ctgcggggcg acgacgaggc gcggaagatc gtgccggaga tgaagcccgc
     2821 gaccgaggag gactggtaca ccgagtacct cgacgccatc ctctcggtgc gcgtggtgaa
     2881 ggacctggat gaggcgatgg atcacatcgc cacctacggc tcgcagcaca ccgacgcgat
     2941 cgtcaccgag gacaaggcgc gcgccaggcg cttcctgcgc gaggtggatt cgagctcggt
     3001 gatggtgaat gcctcgaccc gtttcgccga cggctacgag tacgggctcg gcgcggaggt
     3061 cggcatctcc accgacaagc tgcacgcgcg cggcccggtg ggactcgagg gtctcacctc
     3121 gctcaagtgg gtggtcttcg gcaccggcca gatcag
//
LOCUS       DBNGWZ010000551         7273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3226291, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000551 DBNGWZ010000000
VERSION     DBNGWZ010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3226291"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16CX_17090"
     CDS             <1..320
                     /locus_tag="AC16CX_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014259.1"
                     /translation="RSIREFLWENSKIPMEQMRRAGAPAWIEIDANPVTRASINLDPW
                     PICLKPDNIVLVVAGGGHPTHSYWLQAHSPSVTGRVIRVPETFDRLLADAERDLGPGT
                     ASA"
     gene            complement(360..1151)
                     /locus_tag="AC16CX_17095"
     CDS             complement(360..1151)
                     /locus_tag="AC16CX_17095"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR3014260.1"
                     /translation="MRNPTWPLLALLWLGLTGAAAQQAVPAKIRTQPGLNQVQALDYG
                     ILAGGRIIVKLVFRDEPRRPAIVLVNHHPASSIVLDFADTASALGKEPVEVKHLGVQN
                     LQVMQVGSRARLVIVLDRPLVHEIAVKGKELWITLQRPDAASLRAVEWGLPETASGDA
                     KRSLREVTFQRGESSQGRLMVELTDPATPVEIRQQGKTLLVDFLDSALPAHLQRRLDV
                     QDFGTPIRAVETYRLGNGVRMKIELEEAGEYTAYQVNRQLVVSPR"
     gene            1308..2096
                     /locus_tag="AC16CX_17100"
     CDS             1308..2096
                     /locus_tag="AC16CX_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3014261.1"
                     /translation="MRMPDYQVTGEGKVTVFLLHGIYGSKEYWRYVTERLVARGYRVV
                     AWDAPGYGLSPLPDPFTFDVVAEAGARLIKAQATERNVAFGHSMGGQITPRIRLKVPQ
                     LVHGIVISATIGYFGNRTKEEQEEFVRKRTAPPPPGTDPVTVNMMIVNSMLGPASSGP
                     EVDLVREWASRTPPETVKAAVKAVQSYPETEAVSAIRAVNVPTLLVAGEVDQVGHAAG
                     MKRVADMIAGSEFGVVSGSGHYPWAENPAEFNRFFFGFLDRHFS"
     gene            complement(2124..2537)
                     /locus_tag="AC16CX_17105"
     CDS             complement(2124..2537)
                     /locus_tag="AC16CX_17105"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR3014262.1"
                     /translation="MILSASRQRCTMIGILMAGALALGLAACEQKPSSENLFRNIGDA
                     GPPMEQPANPVGPDSGLAPGAASDAALAAKVKAALTAEPGLKSLKVDVNAADGVVTLF
                     GTADSPKNSHQAAIVALNVEGVRSVKNELVILRGS"
     gene            complement(2672..2845)
                     /locus_tag="AC16CX_17110"
     CDS             complement(2672..2845)
                     /locus_tag="AC16CX_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014263.1"
                     /translation="MPVGDNSTVSRSAGFPINKLISGNFSHLARISHSYGKLAGASRS
                     FTGIVKEIADESE"
     gene            complement(3023..3709)
                     /locus_tag="AC16CX_17115"
     CDS             complement(3023..3709)
                     /locus_tag="AC16CX_17115"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3014264.1"
                     /translation="MVTIKLFTTNSMHGVLDELLPRFERASGHKVSVSYDPARVMLAR
                     IQAGETADLAILGSSAMEELVKQGKIAAASRRVVARCGVGIGVRAGSPGPDIGSVEAF
                     KRALLSAKSIAYTLEGASGIHFAGLIERLGIAEQVKAKAVTRSGGLIGELVAAGEAEI
                     AVQQIPEILAVPGVDFAGPLPQELQMITVSAAGVFADSRQPAAAKELLEFLSTPAAAR
                     VFRAKGLEPA"
     gene            complement(4100..6379)
                     /locus_tag="AC16CX_17120"
     CDS             complement(4100..6379)
                     /locus_tag="AC16CX_17120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3014265.1"
                     /translation="MRREDFRFVAGQGRYTADWNLPGQLHAAFLRADRAHAEIARLDA
                     SRALAHPGVKAVLTGSDAREAGFKSLPNIVTYAGKDGQPLRKPHHPVLAMGRVRFVGE
                     PVAMIVAETAAIAEDARELIEIEYRDLPAVASFDAAVAEGAPQLHADVPGNLAFEYES
                     GDAQAVAAACVRARHVSRLTMVSQRLVGNALEPRACLVAFDPASGRYTIYAPLQGIGS
                     MSGQIAQVTGLHKEKIVIATQDVGGSFGVRGAAYPEYFAAMLAARKLGRPVKWVGTRG
                     EIFMSDFHGRAISLTGEIALDEDGKFLAIRFDDRADLGAYGATFGSFIATRNLTITMG
                     GVYRVPALYARTRLAYTNTVPVSAYRGAGRPDIAYAVERLVDYAAHEHGFDPVELRRK
                     NFVPSEAMPYKTPNAGIYDCGDFEAVMDDALKRADWYGFPKRSAQAKRNGKLRGIGIA
                     TYLEAGGGGAVPKDQVAVEFDRNGAMTLFALTQSSGQGHETVFPQIVAGTLGIDTKHV
                     RFHPGPPGVDLVGNATGGSRGVLGTGSAFRVLGQRLIELARPHAAAKLKVPESELRYD
                     KGRFHGGRRSIGFIELARALAGPKPHPLDTVAEGTFGATYPNGCHIAEVEIDPETGAA
                     SIARYTAVDDLGNVINHTLVEGQVHGGVMQGAGQVFGEHAIYDAPTAQLVTGSFMDYP
                     MPRAGWLRDIEVDDHPVPTSSNALGAKGVGESGATGALPALVNATIDALRPLGIQHLD
                     MPFTPARLWDAIRRARSGE"
     gene            complement(6511..>7273)
                     /locus_tag="AC16CX_17125"
     CDS             complement(6511..>7273)
                     /locus_tag="AC16CX_17125"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3014266.1"
                     /translation="SYGPHHGEEDPLRGRSGSGTIFFSWCNLRCAFCQNWDISQSGIG
                     REVEPREIADMMLELQRLGCHNINFVTPSHVIAQIVAAVHDAARRGLAVPLVYNTSGY
                     DSLEALALLDGIVDIYMPDMKYGDSGLARKYSKARNYAEANRAAVKEMHRQVGDLVLD
                     ARGIAQRGLLVRHLVLPNGIAGTGEVLRFLATEVSRNTYLNLMDQYRPYYCAGEYTEL
                     NRPLTRGEYLEALAAAEQAGLRRLDHRMEYRRGWL"
BASE COUNT         1186 a         2357 c         2436 g         1294 t
ORIGIN      
        1 agcgctcgat acgcgagttc ctgtgggaga actcgaagat cccgatggag cagatgcgcc
       61 gcgccggtgc gccggcctgg atcgagatcg acgccaaccc ggtgacgcgc gcgagcatca
      121 acctcgaccc ctggccgatc tgcctcaagc ctgacaacat cgtgctcgtc gtcgcgggcg
      181 gggggcatcc gacccacagc tactggctgc aggcacactc cccgagcgtg accggacgcg
      241 ttatccgcgt gccggaaacg tttgatcggc tactcgccga cgcggagcgt gacctgggtc
      301 cggggacggc ctctgcttga tttgaaaccg gttacgcctt ccgcgggggg cgtcctgcgc
      361 tatcgcggcg agacgaccag ctggcggttg acctggtatg cagtgtattc cccggcctcc
      421 tcgagctcga tcttcatccg tacgccattg ccgaggcgat aggtctcgac cgccctgatc
      481 ggcgtgccga aatcctgcac gtcgagccgg cgctgcagat gggccggcag cgccgagtcg
      541 agaaaatcca ccagcagcgt tttcccctgc tggcggattt ccaccggcgt cgccgggtcc
      601 gtaagctcga ccatcaaccg gccctggctg ctttccccgc gctggaaggt gacttcgcgc
      661 aggctgcgct tggcgtcgcc ggacgcggtt tcaggcaagc cccattcgac agcgcgcaac
      721 gaagcggcgt cgggacgttg cagcgtgatc cacagctcct tgcccttcac cgcgatttca
      781 tggacgagcg gccggtcgag cacgatcacg agccgcgcgc gggatcccac ctgcatgacc
      841 tgaaggttct gaacgccgag gtgcttgact tcaaccggct ccttgccgag cgcgctcgca
      901 gtgtctgcga aatcgagcac gatgctggag gcggggtgat gattgacgag aacgatggcg
      961 ggtcgtctcg gctcgtcacg gaacaccagt ttgacgatga tcctgccgcc cgcgaggatc
     1021 ccgtagtcga gcgcctgcac ttggttaagc cccggctgcg ttcgaatttt agccgggacg
     1081 gcctgctgcg ccgcagcgcc ggtgaggcca agccagagca acgccaatag cggccatgtg
     1141 ggattacgca tcgcaagcct ccctcccgaa ttttcaatta gacagcggtt gccgtaacgg
     1201 attcatcgag cgtgcgtctg ccggcgcgat ggtccggctt ggcgcgatat gtcacgaaac
     1261 aaaacaggtg tgataattgc agcgctgaaa gcagaaagga agtgagcatg agaatgccag
     1321 actaccaggt caccggcgaa gggaaggtga ccgtgttcct gctgcacggg atctatggct
     1381 cgaaagaata ctggcgttat gtgaccgagc ggctggtcgc gcggggctac cgcgtggtgg
     1441 cgtgggacgc gccgggatac ggtctctcgc cgttgccgga cccgtttacc ttcgatgtcg
     1501 tggccgaggc cggcgcacgg ctgatcaagg cgcaggccac cgagcgcaac gtcgccttcg
     1561 gccacagcat gggcgggcag atcacgccgc gcatccggct gaaggtgccg cagctcgtgc
     1621 acggcatcgt gatctcggcg acgatcggct atttcggcaa ccgcaccaag gaggagcagg
     1681 aggagttcgt ccgcaagcgc acggcgccgc cgcctcccgg caccgatccg gtcacggtga
     1741 acatgatgat cgtgaattcg atgctcggac cggcctcgag cggcccggaa gtcgatctcg
     1801 tgcgcgaatg ggcgtcgcgc accccgcccg agacggtgaa ggcggcggtg aaggccgtgc
     1861 agagctaccc cgagaccgag gcggtgtcgg cgatcagggc ggtgaacgtg ccgacgctac
     1921 tcgtcgcggg cgaagtggac caggtcgggc acgcggccgg catgaagcgg gtggcggaca
     1981 tgattgccgg atcggagttc ggggtcgttt ccggctccgg ccattatccg tgggccgaga
     2041 atccggccga gttcaaccgc ttcttcttcg gatttctcga ccggcatttc tcatgaggaa
     2101 atcgacctgc ggtcgttttc ttgttacgag cctctcagga tcaccagctc gttcttcacc
     2161 gatctgacgc cctccacgtt cagcgccacg atcgcggcct ggtggctgtt cttgggggaa
     2221 tccgccgtgc cgaacagcgt gacgacgccg tcggccgcgt tgacatccac cttcagtgat
     2281 ttcaagcccg gctcggcggt cagagcggct ttcactttcg ccgccagcgc cgcatcactt
     2341 gcggcgcccg gagcgagacc gctatccggt cccaccgggt tggcgggctg ctccatcggg
     2401 ggaccggcgt cgccgatgtt cctgaatagg ttctcgctcg agggcttttg ctcgcatgcc
     2461 gcgaggccga gcgcgagcgc gcccgccatc aggatcccga tcatcgtgca tctttgcctg
     2521 cttgcgctga gtatcatggt tttctccatt cgaaaagcgg tcgtttcacg aggccggtcg
     2581 tcggcggcga ggctcgccgg cgacgcgggc gccgcgccgg caggtcggcg gccatgacac
     2641 caagacagcg gaagcgagct tccgcggccg ttcattcact ctcatcggcg atttccttca
     2701 cgatgcccgt gaatgagcgg ctggcgccgg ccagcttgcc gtatgagtgc gatatccgtg
     2761 ccaggtgtga aaaatttccg gaaatcaatt tgttaatggg aaagccggcc gagcgcgaga
     2821 cggtcgaatt gtcgccaacg ggcaacgcat ggcgggatca gtcctgcagt tggatgattt
     2881 gtcggaaatt tccacttcgc cgaacggcga cagtcagccc cgaacgatga cgacgtgact
     2941 ggaggaactg acggggaaaa ctcaggattg aggaatgagg attgagagac tcgaatcacg
     3001 actcacgatt catcgttttt attcaggcgg gctcgagacc tttggcccgg aacacacgtg
     3061 cggcggcggg tgtcgagagg aactcgagca gctcctttgc cgctgccggc tgccgtgagt
     3121 cggcgaagac gcccgccgct gacacggtga tcatctgcag ctcctgcggc agcggaccgg
     3181 cgaagtcgac gccgggcacg gccaggatct cggggatctg ctgcacggcg atctcggcct
     3241 cgcccgcggc gaccagctcg ccgatgaggc cgccggagcg cgtcaccgcc ttggccttca
     3301 cctgctcggc gatacccagg cgctcgatca ggcccgcgaa gtggatgccg ctcgcacctt
     3361 ccagcgtgta cgcgatggac ttcgcactga gcagtgcccg cttgaacgcc tcgacggatc
     3421 cgatgtcggg tccgggcgag cccgcgcgca caccgatgcc gacgccgcag cgcgccacga
     3481 cgcgccggct cgcagccgca atcttgccct gcttcaccag ctcttccatt gcggacgacc
     3541 cgaggatcgc gaggtccgcc gtctcgccgg cctggatgcg ggcgagcatc accctcgccg
     3601 ggtcatagct caccgaaacc ttgtgcccgc tcgcgcgctc gaaccgcggg agaagctcgt
     3661 ccaggacccc gtgcatgctg ttggtggtga ataacttgat cgtcaccatg attgccgtcc
     3721 gctatgagtg atgggtgata ggtgggacgg agtgatcagt gacgggccca gccggcggcg
     3781 ccggccttga gcgcctgtca ccgatcacgc ttccccggca aatgctccct ggcgtcggcc
     3841 ttgtccatgc actggtagct cgtggtgcac tgcttgaggg agcatgcgtg actcgttctg
     3901 acggcgactt tttttctttc cgcgcacagt ttttcggcgt tgctgcgaac ggcttcggcc
     3961 gctgcgtccg tgaacgggtg ttcgtaggtt agatagttgc tttcgctgac tatgagttgc
     4021 gggcggggcg cgcaaccggc acagaccatc acaagagcga aggagaggaa atttcggatc
     4081 atttcccgga ccaccgcccc tattcccccg aacgcgcccg gcggatggcg tcccacagcc
     4141 gcgccggggt gaacggcatg tcgagatgtt ggatgccgag cggccgcagt gcgtcgatgg
     4201 tcgcgttgac gagcgcgggc agcgcgccgg tggcgccgga ttcgcccacg ccttttgcgc
     4261 cgagtgcgtt gctcgacgtc ggcaccggat ggtcgtccac ctcgatgtcc cgcagccagc
     4321 ccgcgcgcgg catgggatag tccatgaaac tgcccgtcac cagctgcgcc gtgggggcgt
     4381 cgtatatcgc gtgctcaccg aagacctgtc ccgccccctg catgacgccg ccgtgtacct
     4441 ggccctcgac gagcgtgtga ttgatgacgt tgccgaggtc gtccaccgcc gtgtagcgcg
     4501 cgatggacgc ggcgccggtc tcgggatcga tctcgacctc ggcgatgtgg cagccgttcg
     4561 ggtacgttgc gccgaaggtg ccttcggcga cggtgtcgag gggatgcggc ttcggtccgg
     4621 ccagcgcgcg cgcaagctcg atgaaaccga tcgagcggcg gccgccgtga aatctcccct
     4681 tgtcgtagcg cagctcgctc tccggcacct tcaacttggc ggccgcgtgc gggcgcgcga
     4741 gctcgatgag cctctgtccc agcacgcgaa acgcgctgcc ggtgccgagc accccgcgcg
     4801 agccgccggt ggcgttgccg acgagatcca cgcccggagg tccgggatga aagcggacgt
     4861 gcttggtgtc gatgccgagc gttccggcca cgatctgcgg aaagacggtt tcatgcccct
     4921 ggccggacga ttgcgtgagg gcaaacagcg tcatcgcgcc gttccggtcg aactccaccg
     4981 cgacctggtc cttgggcacg gcgcccccgc cgcccgcttc gagataggtg gcgatgccga
     5041 tgccgcggag cttgccgttg cgcttcgcct gcgcgctgcg cttgggaaag ccgtaccagt
     5101 cggcgcgctt taaggcgtcg tccatcacgg cctcgaaatc gccgcagtcg tagatgcccg
     5161 cattgggcgt cttgtagggc atcgcctcgc tcgggacgaa gttcttgcgc cgcagctcga
     5221 ccggatcgaa tccgtgctcg tgggccgcgt agtccaccag ccgctcgacg gcgtaggcga
     5281 tgtcggggcg ccccgcgccc cggtatgcgg agaccggcac ggtattggtg tatgcgagcc
     5341 gcgttcgggc gtacagggcg ggcacgcggt acacgccgcc catcgtgatg gtgaggttcc
     5401 tcgtggcgat gaaggagccg aaggtcgcgc cataggcccc caggtccgcc cggtcgtcga
     5461 agcggatggc gaggaacttc ccgtcctcat ccagcgcgat ttcgccggtg agcgagatcg
     5521 cgcgcccgtg gaaatcgctc atgaaaatct cgccgcgcgt cccgacccac ttgaccgggc
     5581 ggccgagctt gcgcgccgcg agcatcgccg cgaagtactc ggggtatgcg gcgccgcgca
     5641 cgccgaaact gccgccgacg tcctgggtgg cgatcacgat cttttccttg tggaggccgg
     5701 tgacctgcgc aatctggccg ctcatgctgc cgataccctg gagcggcgcg taaatggtgt
     5761 agcggccgct cgcggggtcg aacgccacga gacacgcgcg cggctccaga gcgttgccga
     5821 ccaggcgctg gctgaccatg gtgagcctgc tcacgtgtct cgcccgcacg caggcggccg
     5881 cgaccgcctg cgcgtcgccg gattcatatt cgaacgcgag gttgcccgga acgtcggcgt
     5941 ggagctgcgg cgcgccttcc gccaccgcgg cgtcgaagct cgcgacggcg ggaaggtcgc
     6001 ggtattcgat ttcgatcaac tcacgcgcgt cctctgcgat cgccgcggtt tccgcgacga
     6061 tcattgcgac cggctcgccg acgaagcgca cgcgccccat cgcgagcacg gggtgatggg
     6121 gcttgcgcag cggctggccg tccttgcctg cgtaggtgac gatgttgggc agcgacttga
     6181 agccggcttc gcgggcatcg cttcccgtca acactgcttt cacgccggga tgggccagcg
     6241 cgcgggaagc atcgagccgg gcgatctcgg cgtgcgcccg gtcggcccgc agaaaagccg
     6301 cgtgcaactg gcccggcaga ttccaatcgg cggtgtaacg accttggccg gctacgaaac
     6361 gaaaatcttc gcggcgcata gtccctttcc tgcgctggac gtgcgcggat tctcgcacag
     6421 ttcaggggga gaaagtgacg agagggggtg acgagcaggg gcgcaaaaaa gagtgacgaa
     6481 aatccactcg tcactcactt cggctcaccg ctacagccag ccccgccggt actccatccg
     6541 gtggtcgagt cgccggagcc ccgcctgctc ggcagccgcg agcgcctcca gatactcccc
     6601 acgggtgagc ggccggttga gctcggtgta ctcgccggcg caatagtacg ggcggtactg
     6661 atccatcagg ttgaggtagg tgttccgcga gacctcggtt gccaggaagc ggagcacttc
     6721 acccgtcccc gcgatgccgt tcggcaggac gagatgccgt acgagcaggc cgcgctgcgc
     6781 gattcccctg gcgtcgagca cgagatcgcc cacctgccgg tgcatctcct tcacggctgc
     6841 acggttcgcc tcggcgtagt tacgcgcctt cgagtacttg cgcgcgaggc ccgaatcgcc
     6901 gtacttcatg tccggcatgt agatgtcgac gatgccgtcg agcagcgcga gcgcttccag
     6961 gctgtcgtag ccgctggtgt tgtagacgag gggcaccgcc aggccgcgcc gtgccgcgtc
     7021 gtgcacggcg gcgacgatct gggcgatgac atggctcggc gtgacgaagt tgatgttgtg
     7081 gcagccgagg cgctgcagct cgagcatcat gtccgcgatc tcgcgcggct ccacttcgcg
     7141 gccgatgccg ctctgggaga tgtcccagtt ctggcaaaac gcgcagcgca ggttgcacca
     7201 gctgaagaag atcgtcccgg acccggaccg gccgcgcagc ggatcctcct cgccgtggtg
     7261 cggcccgtag ctc
//
LOCUS       DBNGWZ010000552         7877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2470239, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000552 DBNGWZ010000000
VERSION     DBNGWZ010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2470239"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC16CX_17130"
     CDS             <1..227
                     /locus_tag="AC16CX_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR3014267.1"
                     /translation="GSLDESNALYEARRCLSCGNCFECDNCYGVCPDNAVIKLGPGKR
                     FEFNYDYCKGCGLCAAECPCGAINMVPEKI"
     gene            complement(264..986)
                     /locus_tag="AC16CX_17135"
     CDS             complement(264..986)
                     /locus_tag="AC16CX_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014268.1"
                     /translation="MAIYEARLARRETVAEGTMAFYFSKPSGFRHQAGQSLLMTLINP
                     PETDSEGDARSFTIASAPQEAELMIATRMRDTAFKRVLKTAPVGTTVRIDGPNGEMVL
                     HDDPARPAVFLAGGIGITPFLAMARHAANERLPYHLFLFYSNRRPEDAAFLAELRKME
                     RLNPNYHLIATMAEPEKSAHPWSGEKGFIRREMLERHLPDLTSPVYYLAGPPAMTMAM
                     QQMLQDVGIREQDMRSEEFYGY"
     gene            complement(1005..1226)
                     /locus_tag="AC16CX_17140"
     CDS             complement(1005..1226)
                     /locus_tag="AC16CX_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR3014269.1"
                     /translation="MARIVKRTYSEPYKLIIGGEEKYLCRCGLSKNQPFCDGSHKLAK
                     SEETGKLYWYDDAGQRHESTDAFAGIRTF"
     gene            complement(1366..2025)
                     /locus_tag="AC16CX_17145"
     CDS             complement(1366..2025)
                     /locus_tag="AC16CX_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014270.1"
                     /translation="MKPIQQSVIAASIVAAFLAPAAPVFAQHTEHVVVAQAPAATKVA
                     ETGAALRDLWVGHIFWVRNVVIDTFAGNKKAAAAAEVEVVANAKQIAAAIEPYYGKEA
                     SEKLFGLLAGHYGAVKQYLEANAAGSKAKQDAAVKKLTDNAGEIAKFLSGANPNLPFD
                     TLNGLLIAHGGHHIQEIQQLKSKQYAQEAQTWEAMKKHMYVIADALAGAIVKQFPAKF
                     T"
     gene            2207..2842
                     /locus_tag="AC16CX_17150"
     CDS             2207..2842
                     /locus_tag="AC16CX_17150"
                     /inference="COORDINATES: protein motif:HMM:NF037269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3014271.1"
                     /translation="MLDILGDRQKQLLELLLKKKSGLTVDELSQGLKITRNAVRQHLA
                     ALESDGLIALGSTRPSGGRPQQLYALTEKGKEVFPRHYSWFAQLVIEAIKREHGAEGL
                     RERLASIGSSVAAQLRAQNPGLETREQKVEKLAAVMEQLGYNARTAAVTDKAPVIEAD
                     NCVFHELAVKNPEICHFDLAMMGTFTDSKVDHQECMARGGNVCRFKFTARK"
     gene            2842..3282
                     /locus_tag="AC16CX_17155"
     CDS             2842..3282
                     /locus_tag="AC16CX_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR3014272.1"
                     /translation="MKDAEHGGLKAAKRARARGPRRTPGLKRAYERPSPSDGMCVLVD
                     RLWPRGLSKKQLAIGLWLKDVATSDALRRWYGHDPRRWESFAVKYRAELARRTDLLRT
                     LDELQHRRRITLVYGARDETRNNAVVLREVLRERRFAGPRTRQK"
     gene            3314..4528
                     /locus_tag="AC16CX_17160"
     CDS             3314..4528
                     /locus_tag="AC16CX_17160"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR3014273.1"
                     /translation="MVDRTVAMDNWNEIKAWRKTRRAELITARTAFKPEQRKAWNERI
                     TALLGASFAFPPGTVIGFCWPYKGEFDARFAVRCWRDHGAVAALPEVVERTRPLQFHK
                     WWPGAPMRPGVYGIPVPDGTEILLPDVAVVPMNGFDEQGYRLGYGGGYFDRTLAVLDR
                     KVLAIGVSFEALRLPTIHPQPHDIPMDFVVTEAGIYRAGGRKLALLSAAEYSAAAKSL
                     LESHGLPRDRRSLAANAAANPGAGGYSSPSCYAHEIAPGYFGESPTMPAEEFVGLLNV
                     LLEAERAGAKTLAAFLNDYERGTPTWRRLAAVRRDEANNCAILIDLIRRVNGAPSAAT
                     GDFLGKALAVEGKAARLHFLNRGQKWVARKISEALPYLEQDFVRGALVAMHESHLINI
                     EACDALMEALEA"
     gene            4728..6005
                     /locus_tag="AC16CX_17165"
     CDS             4728..6005
                     /locus_tag="AC16CX_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR3014274.1"
                     /translation="MPADAPATDRFGNPIDPIVRYARGAILKGTDEEVRRMLRARGMV
                     GERMRARGKDSIYDLSGMNRGGGITAQDTAHLTSHVPFFERFEGRTEPLALKHMGADP
                     QRHQALILNRVSAVNFIALTTVLAPGDRVFGLAPAGGVTHPSCVRPIEMAGAKIKELF
                     SFADLEQAWREEGAPRLLLITPISASKRHLPLDDFRRALKLPRAPHTLVYVDDAHMAS
                     RIGFFEEPRTFEVGEIDLAVCSADKHVAGPRAGVFVGRRDLVTAIGSRAYELGLEAQA
                     GQYVGVANALRDFDPKVIKRAGELARQLVEVLAAQYGQGRAYLGGPGVSVSGEDVLDI
                     ARESRGTNEKPELVPVEAAGLVAMEMLERDGVLTIGAVSMPGSAPVVRLMMYPDGHKL
                     GVERIAASLENGIARLAEVLDDVDEARAQLLGE"
     gene            6010..6981
                     /locus_tag="AC16CX_17170"
     CDS             6010..6981
                     /locus_tag="AC16CX_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014275.1"
                     /translation="MRISMLTTAIAIACAGFAAFAAAQSYPSRPVRLIVPFPAGGGSD
                     IVGRILAARLTEQLKQQVYVDNRGGAGGSIGTEAAVRSAPDGHTLVLASTSEIAVNPA
                     IYTKLAYDTVRDLAPVALVASTPVVVVVHPSLPVKSVKELIALAKARPGDINMASAGN
                     GTFTHLSGELFKSVTGARMTHVPYKGAPTALSDLVAGQVQIMFSSLPAATGLINGGRI
                     RAIAVSTAKRAENLPAVPTVIESGVPPYEVTYWYGTFVPAATPKEILAQLYNEVAQAL
                     RAPEVAGGLAKQGATPGTLTQPQFTDFVKSEHARWGKVAKATGVKLD"
     gene            complement(7026..7580)
                     /gene="ahpD"
                     /locus_tag="AC16CX_17175"
     CDS             complement(7026..7580)
                     /gene="ahpD"
                     /locus_tag="AC16CX_17175"
                     /EC_number="1.11.1.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0160319 - lipoyl-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase, AhpD family"
                     /protein_id="HIR3014276.1"
                     /translation="MLLQELRDRLPDYAKDIRLNLGSLASEQVLSEKQKAGAFIASAL
                     GARNPEVAAAAMAEFAPQLDAKELAAAQAAAAVMAMNNVYYRFLHLVGNEEYGKLPAK
                     LRMNTIATHGISRADFELWCLAVSAVNGCGSCIASHERAVREAGMSAEQVQAAVRIAA
                     VVNAAAALLDAATAATPEPVTLAA"
     gene            complement(7686..>7877)
                     /locus_tag="AC16CX_17180"
     CDS             complement(7686..>7877)
                     /locus_tag="AC16CX_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3014277.1"
                     /translation="RKEGVALRATFIVDPEGMIRFASVHDLSVGRNVDEVLRTLDALQ
                     SDELCPCNWQKGDAFLKAA"
BASE COUNT         1406 a         2501 c         2528 g         1442 t
ORIGIN      
        1 tgggcagcct cgacgagtcg aacgcgctct acgaggcgcg gcgctgcctc tcgtgcggca
       61 actgcttcga gtgcgacaac tgctacggcg tgtgccccga caacgccgtg atcaagctcg
      121 gccccggcaa gcgcttcgag ttcaactacg attactgcaa gggctgcggg ctgtgcgccg
      181 ccgaatgccc gtgcggcgcg atcaacatgg tgccggagaa gatctgatcc gcatcccgtc
      241 taccggaaac actgcgtgtg cgttcagtag ccgtagaatt cctcggagcg catgtcctgc
      301 tcgcggatgc cgacatcttg cagcatctgc tgcatcgcca tggtcatcgc cggcggcccc
      361 gctaagtaat agaccgggct cgtgaggtcg ggcaggtgcc gttccagcat ctcgcgccgg
      421 atgaagcctt tctcgcccga ccatgggtgc gccgatttct ctggttcggc catggtcgct
      481 atcagatggt agtttgggtt caaccgctcc atcttccgca gctcggcgag gaacgccgcg
      541 tcttctggac ggcggttgga ataaaagagg aaaaggtgat atggcagccg ctcgttcgcc
      601 gcatgccgcg ccatcgctag gaatggcgtg atgccaatgc cgccggctag aaacacggcg
      661 ggccgcgccg gatcgtcatg caacaccatc tcgccgttgg ggccgtctat tctgaccgtc
      721 gttccgaccg gcgccgtttt cagcacacgc ttgaacgcag tgtcgcgcat gcgcgtcgct
      781 atcattaatt cggcttcttg gggcgcgctg gcaatcgtga aggagcgtgc atcaccttcg
      841 ctatcggttt ccggcggatt gatgagcgtc atcaataatg actgtccggc ctgatgccga
      901 aatccggagg gtttggaaaa ataaaacgcc atcgtgcctt ccgccacggt ctcgcgccgc
      961 gccaaccgtg cttcataaat tgccatgatg aactccgtcg ccgctcagaa tgtgcgaatt
     1021 cctgcaaagg catcggtgga ttcgtggcgt tgtcctgcat cgtcgtacca gtaaagcttg
     1081 ccggtctcct cgctcttggc gagcttgtgc gagccatcgc agaacggctg gttcttcgat
     1141 agtccgcaac gacagagata cttttcctcg ccaccgatga tcaacttgta cggctcagag
     1201 taagtgcgtt ttacgatgcg tgccatgaca gcttcctttc taaaatagtc ttgatatggt
     1261 tgcgttctaa aagatcatgc gtcaggactt gccatgcgaa tttggtttcg agccgtgcgc
     1321 tcccgagcga cggcgtgcgg cgctccgcac gccgtggccg ccccatcagg tgaacttcgc
     1381 gggaaactgc ttgacgatcg ccccggcgag cgcatcggcg atcacgtaca tgtgcttctt
     1441 catcgcttcc caggtctgcg cttcctgcgc gtactgcttg ctcttgagct gttggatttc
     1501 ctggatgtga tggccgccgt gggctatcag taatccgttc agcgtgtcga acggcaggtt
     1561 aggattggct ccgctgagga atttcgcgat ttctccggcg ttgtcggtca gcttcttgac
     1621 agccgcgtcc tgcttcgcct tgctacctgc agcgttcgcc tcgaggtatt gcttgaccgc
     1681 accgtagtgt ccagcgagca ggccgaatag cttctcggaa gcctccttgc cgtaatacgg
     1741 ctcgatcgcg gctgcgatct gcttggcgtt ggcgacaact tctacctccg ccgctgccgc
     1801 tgctttcttg ttccccgcga aggtgtcgat gaccacatta cgcacccaaa agatgtggcc
     1861 tacccaaaga tcgcgcagcg cggcgccggt ttcggccacc tttgtcgctg ccggtgcctg
     1921 tgcaacaaca acatgttcgg tgtgctgcgc aaacaccggc gcagccggtg ccaagaacgc
     1981 agccacgatt gaggctgcta tgacagattg ttgaatcggt ttcattgcgt tttcctttac
     2041 aagttgattg aggttggaat ttcggtgttg cttcccgaga ctacggcact aactatatat
     2101 tgtcaagtta aatatgtata tattgacatt agtgtcagat cacctataat tcatgaattc
     2161 ggtaggcgcg ctgcggaacg cacgccaagc tagaaaggga tgccaagtgc ttgacatatt
     2221 gggcgatagg cagaagcagc tgctcgaatt gctgctcaag aaaaagtctg gcctgacggt
     2281 ggacgagctc tcccaagggc taaagatcac gcgcaatgcg gtgcggcagc acctcgccgc
     2341 gctggagagc gacgggctaa ttgcgctggg ctcgacccgc ccttcgggag ggcggccgca
     2401 gcagctctat gcgctgaccg aaaaaggcaa agaagtcttc ccgcgccact attcctggtt
     2461 cgcgcagttg gtgatcgaag cgatcaagcg ggaacacggc gccgaaggct tgcgcgaacg
     2521 gctcgcctcg attggctcga gcgtcgcggc ccagctgcgc gcccagaatc ccgggctcga
     2581 gactcgcgaa caaaaagtcg aaaagctcgc cgcggtaatg gagcagctcg gctacaacgc
     2641 gagaaccgcg gccgtcacgg acaaggcacc ggtgatcgag gccgataact gcgtcttcca
     2701 cgagctcgcc gtgaagaacc cagaaatctg ccacttcgac ctggcaatga tgggcacctt
     2761 cactgacagc aaagtggacc accaggagtg catggcgcgc ggcggcaacg tctgccgctt
     2821 caagttcacc gcaaggaaat aatgaaggac gcagagcatg ggggcctcaa ggccgcaaag
     2881 cgcgcacgag cgcgcgggcc gcgtcgcacg ccgggtctga agcgcgccta cgaacggcct
     2941 tctcccagcg acgggatgtg cgtcctcgtc gaccggttgt ggccgcgcgg cctgagcaag
     3001 aagcagctgg cgatcggtct ctggctcaag gacgtggcga caagcgatgc gctgcgccgc
     3061 tggtacgggc acgatccccg tcgctgggaa tcatttgcag tgaagtaccg cgccgagctt
     3121 gcacgaagga ccgatctgct gcgcacgctg gatgaactgc agcaccgcag gcggatcaca
     3181 cttgtctacg gcgcgcgcga cgaaacccgc aacaacgcag tcgtgctgcg cgaggtgctt
     3241 agagagcgtc ggttcgccgg accgcgcacg cggcagaaat aaaagggata gcaacaagaa
     3301 cgaggcgttt gcggtggttg atcgaactgt ggcgatggac aactggaacg agatcaaggc
     3361 gtggcgcaag acgcggcgcg cggaactcat caccgcgcgg actgccttca agcccgagca
     3421 gcgtaaggcc tggaacgaac ggattacggc gctgctggga gcgagcttcg cgtttccgcc
     3481 aggcacggtg atcggtttct gctggccgta caagggcgag ttcgacgcgc ggttcgcggt
     3541 ccggtgctgg cgcgaccacg gggcagtcgc cgcattgccg gaagtggttg agaggacgcg
     3601 tccgctgcag ttccacaagt ggtggccggg agcgccgatg aggccgggtg tctacggcat
     3661 cccggtgccc gatggcaccg agattctgct gcccgatgtg gcggtggtgc cgatgaatgg
     3721 tttcgacgag cagggctacc gcctgggcta cggcggcggc tacttcgacc ggacactggc
     3781 cgtgctcgac cggaaggtac tggcaatcgg ggtcagtttc gaggcattgc gcttgcccac
     3841 gattcatccg cagccgcacg acatcccgat ggatttcgtc gtgaccgaag cgggtatcta
     3901 ccgtgcgggg ggcaggaagc ttgcgctgct ttccgcggcg gagtattccg ccgccgcgaa
     3961 atcgttgctg gaaagccacg gtttgccgcg cgatcgccgt tcgcttgcag cgaacgcggc
     4021 ggcgaaccct ggtgcaggtg gctacagctc gccgtcctgc tatgcgcacg agatcgcgcc
     4081 gggctacttc ggagagtcgc cgacgatgcc ggcggaggaa ttcgtcggat tgctcaatgt
     4141 gttgctggaa gcggagcgtg ccggcgccaa gacgctcgcg gcttttctga atgattacga
     4201 gcgcggcacg cctacgtgga ggcggctcgc cgcagtgcgg cgtgacgagg cgaacaactg
     4261 cgcaatcctg atcgatctga tccggcgtgt gaatggcgcg ccgagcgcgg caaccggaga
     4321 tttcctgggt aaggcgctgg cggtggaggg caaagcagcg cggctgcatt tcctcaaccg
     4381 cgggcagaaa tgggtcgcgc gcaagatcag cgaagcgttg ccgtacctgg agcaggactt
     4441 cgtgcgcggt gctcttgtcg caatgcacga gtcccacctg atcaacatcg aagcgtgcga
     4501 cgcgctgatg gaagcgttgg aagcgtaaag caggagcgac aggaagacct actgtctctg
     4561 ccttctgctc actgccagcc gatcacgtcg tagccgcggt cgcgcaggct gcgctgcgcg
     4621 aaatccgtaa agccggagcg gttccgtcgc gccctgcgcc ttgacgctgc aacgcggagc
     4681 ggcctaagct tggcccacac cttgcgcata taccccgagg ccagcccatg cccgccgacg
     4741 cccccgcaac cgaccgcttc ggcaacccca tcgatcccat cgtgcgctac gcgcgcggcg
     4801 ccatattgaa gggcaccgac gaggaggtgc ggcgcatgct gcgcgcgcgc ggcatggtgg
     4861 gcgagcgcat gcgcgcgcgg ggcaaggaca gcatctatga cctctcgggc atgaaccgcg
     4921 gcggcggcat cacggcgcag gacacggcgc acctcaccag tcacgtgccg tttttcgagc
     4981 gctttgaagg ccgcaccgag ccgctcgccc tgaaacacat gggcgccgac ccgcaacggc
     5041 accaggcgtt gatcttgaac agggtttcgg cggtgaattt catcgcgctc acgaccgtgc
     5101 tcgcgcccgg ggatcgcgta ttcggtcttg cgcccgcggg cggcgtaacg catccctcgt
     5161 gcgtgcgccc gatcgagatg gcgggcgcga agatcaagga gctcttctcc ttcgccgacc
     5221 tcgagcaagc ctggcgcgaa gaaggcgcgc cgcggctgct gctgatcacg ccgatctcgg
     5281 cctccaagcg ccatcttcca ctcgacgatt tcaggcgcgc gctgaaactg ccgcgcgcgc
     5341 cgcacacgct ggtctacgtg gatgacgcgc acatggcctc ccgcatcggc ttcttcgagg
     5401 aaccgcgcac cttcgaggtg ggcgaaatcg atcttgcggt gtgctccgcc gacaagcatg
     5461 tcgcgggccc gcgggcgggc gtcttcgtcg gacgcaggga cctggtgacc gccatcggct
     5521 cgcgcgccta tgagctgggg ctggaagcac aggccgggca gtacgtcggg gtcgccaacg
     5581 cgctgcgcga cttcgatccg aaagtcatca agcgcgcggg cgagcttgcc aggcagctgg
     5641 tggaggtgct cgcggcgcaa tacggacaag gacgcgccta cctgggcggc cccggcgttt
     5701 cggtctcggg cgaagatgtg ctcgacatcg cgcgcgagag ccgcggaacg aacgaaaagc
     5761 ccgagctggt gccggtcgag gcggcgggac tggtggcgat ggagatgctg gagcgggacg
     5821 gtgtgctcac catcggcgcg gtttcgatgc ccggcagcgc ccccgtcgtg cggctcatga
     5881 tgtatcccga cgggcacaag ctcggcgtgg agcgcatcgc cgcgtccctc gagaacggca
     5941 tcgcacggct cgccgaggtt ctcgacgacg tggacgaggc gcgcgcgcaa ctgctcgggg
     6001 agtgaaacga tgaggatcag catgctcacc acggcgatcg ccatcgcctg cgcggggttc
     6061 gcagccttcg cggccgcgca atcctatccg tccaggcccg tgcggctcat cgtgcccttc
     6121 cccgccggcg gcggctcgga catcgtcggc cgtatcctcg ccgccaggct caccgagcaa
     6181 ttgaagcagc aggtatacgt ggacaaccgc ggcggggccg gcggctcgat cggcaccgag
     6241 gcggcggtgc ggtccgctcc tgacggccac acgctggtgc ttgcttccac ctccgagatc
     6301 gccgtcaacc ccgcgatcta caccaagctc gcctacgaca cggtgcgcga cctcgcaccg
     6361 gttgcgctcg tcgcctccac gcccgtggtc gtcgtcgtgc atccctcttt gccggtgaaa
     6421 agcgtcaagg agctgatcgc gctcgcgaaa gcgcggcccg gtgacatcaa catggcctcg
     6481 gccggcaacg gcaccttcac ccatctctcg ggcgagctgt tcaagtcggt gaccggcgcc
     6541 aggatgacgc acgtgccgta caagggcgcg ccgaccgcgc tctccgatct cgtcgcaggc
     6601 caggtgcaga tcatgttctc ctccctcccg gctgccaccg gcctcatcaa cggcggccgc
     6661 attcgggcga tcgcggtttc cacggcgaag cgcgcggaga acctgcccgc agtgccgacc
     6721 gtgatcgagt ccggcgtccc gccctacgag gtcacctact ggtacggcac tttcgtgccg
     6781 gcggcgacac cgaaggagat actcgcccag ctttataacg aggtcgccca ggcactgcgc
     6841 gcaccggagg tggccggggg tctcgccaag cagggcgcca cccccggaac gctgacgcag
     6901 ccgcaattca ccgacttcgt gaagtccgag cacgcgcgct ggggcaaggt cgcgaaagcg
     6961 accggcgtca agctcgactg atccaccggc ggcatcgggc cgccgcgccg ggtgcgtgac
     7021 ggcgcttacg cggcgagcgt gaccggctcc ggcgtggccg ccgtcgcggc gtcgagcagc
     7081 gcggcggcgg cgttgaccac cgcggcgatg cgcaccgcgg cctgcacctg ctcggcgctc
     7141 atgcccgctt cgcgcaccgc ccgttcgtgc gacgcgatgc acgatccaca gccgttgact
     7201 gcggaaacag cgagacacca cagctcgaaa tcggcgcggg agattccatg ggttgcgatc
     7261 gtgttcatcc tcagcttcgc ggggagcttg ccgtactcct cgttccccac gagatgcagg
     7321 aaacgatagt aaacattgtt catggccatg acggcggcgg ccgcctgcgc tgcggccagc
     7381 tccttcgcgt cgagctgcgg ggcgaactcc gccatggccg cggcggcaac ttccgggtta
     7441 cgggcgccca gcgcggacgc gatgaacgcc cccgccttct gtttttcgct caggacctgc
     7501 tcggatgcga gcgagccaag gttcagcctg atgtccttcg catagtccgg caaacggtct
     7561 ctcagctctt gcaacaacat gttcgtctcc tttcgtcgag ttctcagcca tcagctgtca
     7621 gctatcagcc ggagcagctt gccgtttcac gctgtccgct ggccactgac tgctgaaagc
     7681 tgccgctatg cagccttgag aaaggcgtcg cccttctgcc agttgcacgg gcatagctcg
     7741 tcgctttgca aggcatcgag cgtgcgcagc acttcgtcca cattgcgccc gacgctcaag
     7801 tcgtgcaccg acgcgaagcg gatcatgccc tcggggtcca cgatgaaggt cgcgcgcagc
     7861 gccacgcctt ccttgcg
//
LOCUS       DBNGWZ010000553         5504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2343790, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000553 DBNGWZ010000000
VERSION     DBNGWZ010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2343790"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /gene="folE"
                     /locus_tag="AC16CX_17185"
     CDS             <1..381
                     /gene="folE"
                     /locus_tag="AC16CX_17185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR3014278.1"
                     /translation="MVIVKDIELYSLCEHHVLPFFGKCHVAYIPNGYIVGLSKIPRVV
                     DAFARRLQVQERLTVEIRNCIEETLKPVGVAVVIEAQHLCMVMRGIQKQHSIATTSAF
                     TGEFNTEKTRAEFMRLIGRGNPVG"
     gene            493..1767
                     /gene="ribA"
                     /locus_tag="AC16CX_17190"
     CDS             493..1767
                     /gene="ribA"
                     /locus_tag="AC16CX_17190"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR3014279.1"
                     /translation="MSAKTSEYIVESILPDVRRGRMVILVDDEDRENEGDLFVAAERA
                     TPEAISFMATHGRGLISLALTEERMRSLGLSLITDDTGNQTRFGTAFATPIDAREGVG
                     SGMSAHDRARTIAVAIADNAKPTDLIRPGRLQTLRAVDGGVLARRGHTEAAVDLARLA
                     GLKPAGVICEIMKDDGTMARMPDLERFARGHGIRILKIADLIAYRQNERQIRRRAETR
                     LPTRKAGEFRCMVYSDNLETQAFVALVKGEVRPDAPVLVRLHSQCLTGDVFGSERCDC
                     GEQLDTALAMIEKAGAGVLVYMFDEGRGIGLLNKIRAYALQDQGRDTVEANHALGFAA
                     DMRDYKSGAHILFDLGARKVRLMTNNPDKVRQLEQYGLTVTERVPIEVPPRKSNRDYL
                     RTKRAKFGHLLSLVAEKQDSGSRIQDSGKKRR"
     gene            1791..2204
                     /locus_tag="AC16CX_17195"
     CDS             1791..2204
                     /locus_tag="AC16CX_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR3014280.1"
                     /translation="MASYVDSVLISGESVLHRGRVSLWPHALKLALGIVLLPLFGLGL
                     VFLVWVYIVYRTTEIAITNKRIIAKFGFVSRSTIEINLPKIESLQVDQSVAGRLFSYG
                     TLVVAGAGTPNLRIPGIADPLRFRKHFMEATDRPQ"
     gene            2230..2520
                     /locus_tag="AC16CX_17200"
     CDS             2230..2520
                     /locus_tag="AC16CX_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HIR3014281.1"
                     /translation="MGRKPRIEVEYCTQCRWLLRAGWTAQELLTTFEAEIGEVALIPG
                     SGGIFEVRVDGETVWSRKERGRFPELAELKQLVRDRVAPGKDLGHSEKNRER"
     gene            complement(2634..3203)
                     /locus_tag="AC16CX_17205"
     CDS             complement(2634..3203)
                     /locus_tag="AC16CX_17205"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR3014282.1"
                     /translation="MALTAGKAVVTINGGKPFTMQAGQSSPEGVRLIEATSESATFEI
                     GGKRQTLTMGQTISLGGGPVTAQRATLTADAGGHFVTTAAINGVSMKFMVDTGASLVT
                     INSGDAKRAGINYLAGQKAVLQTANGTTPAYRVKLDTVRLGDITLNNVDGVVVEGNIM
                     GELGLLGLSFLNRVEMRRDGATMTLTRRY"
     gene            3309..4028
                     /locus_tag="AC16CX_17210"
     CDS             3309..4028
                     /locus_tag="AC16CX_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3014283.1"
                     /translation="MKPVVIFRHTESEGPGYFATYLTRRDIPWRVIRIDEGEAVPGNP
                     REFSGLAFMGGPMSVNDDLPWITPVLRLIRSAADAGVSTLGHCLGGQLMAKALGGEVT
                     RNPAKEIGWGRVDVLQNDVAGRWFGASLRSFNSFHWHGETFSIPPGAVRIASSPYCGN
                     QAFALGPHLGMQCHVEMTPELVRTWCQDWEQEVESLARRMPSVQTPAEMMEAVEEKVR
                     TLNSIADRLYDCWTAGLKLGD"
     gene            complement(4126..4617)
                     /locus_tag="AC16CX_17215"
     CDS             complement(4126..4617)
                     /locus_tag="AC16CX_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR3014284.1"
                     /translation="MPSFDIVSEVNHVEIRNAIEQCNKEIANRFDFKGSDARVEINDK
                     EKTLTAFADDEFKLRQLRDVLTGRLAKRGVDIRALEPGKIEPISGNKVKETIKVREGI
                     EQERGKAIVKLIKDSKLKVQAGIQGDAVRVSGPKKDALQETIALVRKSVTDIPLQFKN
                     FRD"
     gene            5132..>5504
                     /locus_tag="AC16CX_17220"
     CDS             5132..>5504
                     /locus_tag="AC16CX_17220"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014285.1"
                     /translation="MRNLFVATVIAATWCSGTLAQTQSYPAKPVRILVGFAPGGGIDI
                     VTRLYAHRLTEVMGQSFVVENRPGAGGTIATDLVAKAPPDGHTLIGLSVTHSINASLY
                     SKLPYDSIKNFTPVSPTALLAD"
BASE COUNT         1039 a         1740 c         1730 g          995 t
ORIGIN      
        1 atggtgatcg tgaaggacat cgagctctat tcgctgtgcg agcatcatgt cctgccgttc
       61 ttcggcaagt gccacgtcgc ctacatcccc aacggctaca tcgtcgggct ctcgaagatc
      121 ccgcgcgtgg tggacgcctt cgcgcggcgg ctgcaggtgc aggagcggct gacggtcgag
      181 atccgcaatt gcatagagga aaccctgaag cccgtcggcg tcgcggtggt gatcgaggcg
      241 cagcacctgt gcatggtgat gcgcgggatc cagaagcagc actcgatcgc gaccacttcc
      301 gcattcaccg gcgaattcaa caccgagaag acgcgcgccg agttcatgcg gctgatcggc
      361 cgcggcaacc cggtcggctg atggtgccgg tgactaagtg attgagtgac taggtaacta
      421 agtaacgacg atcggatcgc ccagcttttc tcagtcactt agtcactcag tcactaaatc
      481 actcgcgaag caatgagcgc gaagaccagc gaatacatcg tcgagagcat cctgcccgac
      541 gtgcgccggg gccggatggt aatcctcgtg gacgacgagg atcgcgagaa cgagggcgat
      601 cttttcgtcg ccgccgagcg cgcgacgccc gaggcgatca gcttcatggc gacgcacggc
      661 cgcggtctga tctcgctcgc gctgacggaa gagcgcatgc ggagcctcgg gctctcgctc
      721 atcaccgacg acaccggcaa ccagacgcgg ttcggcaccg cgttcgccac gccgatcgac
      781 gcgcgcgagg gagtcggctc gggcatgtcg gcgcacgacc gggctcgcac catcgcggtc
      841 gccatcgcgg acaacgccaa gcccaccgac ttgatccgcc ccgggcggct gcagacgctg
      901 cgcgcggtgg acggcggcgt gctggcccgc cgcggccata cggaagccgc ggtggatctc
      961 gcgcggctcg ccgggctcaa gcccgccggt gtaatctgtg agatcatgaa ggacgacggc
     1021 accatggcgc gcatgccgga cctcgagcgc ttcgcgcgcg gccacggcat cagaatcctc
     1081 aagatcgccg acctcatcgc ctatcgccag aacgagcgcc agatccggcg gcgggcggag
     1141 acccggctcc ccacgcgcaa ggcgggcgag ttccgctgca tggtctacag cgacaacctg
     1201 gagacgcagg cgttcgtggc gctggtaaag ggcgaggtga gaccggacgc gccggtactg
     1261 gtccggttgc actcgcagtg cctcacaggc gacgtcttcg gctccgagcg ctgcgattgc
     1321 ggcgagcagc tcgatacggc gcttgcgatg atcgaaaagg cgggcgccgg cgttctggtc
     1381 tacatgttcg acgagggccg cggcatcggg ctgctcaaca agatccgcgc ctacgccctc
     1441 caggaccagg gccgggacac agtcgaggcc aatcatgcgc tcggtttcgc cgccgacatg
     1501 cgtgactaca agtccggtgc gcacatcctg ttcgacctcg gggcgcgcaa ggtccgcctg
     1561 atgaccaaca acccggacaa ggtgcggcag ctcgagcagt acggcctgac cgtcaccgaa
     1621 cgggttccga tcgaagtgcc cccgcgcaag tccaaccgcg actacctgcg caccaagcgc
     1681 gccaagttcg gccacctgct gtcgctcgtc gccgagaagc aggattccgg gtccaggatt
     1741 caggattcag ggaagaaacg caggtaacct gcgtttcccg atcccggaat atggcgtcgt
     1801 acgtcgacag cgttctgatc agcggcgagt cggtgcttca tcgcggacgg gtttcgctct
     1861 ggccgcacgc gctgaaactc gccctcggca tcgtgctgct gcctttgttc gggctcggcc
     1921 ttgtgttcct ggtctgggtt tacatcgttt acaggaccac agaaatcgcg atcaccaaca
     1981 agcgcatcat cgccaagttc ggtttcgtca gccgcagcac gatcgagatc aacctgccca
     2041 agatcgagag cctacaggtg gaccagagcg tggccgggcg actcttcagc tacggcaccc
     2101 ttgtcgtcgc cggggcggga acgccgaatc tgcggatccc ggggatcgcg gacccgttgc
     2161 ggttccgcaa gcatttcatg gaagcgaccg accggccgca gtagcgccgg gaccgggacg
     2221 tgccgagcaa tgggccgcaa gccgagaatc gaggtcgagt actgcacgca atgccgctgg
     2281 ctgctgcggg cgggctggac cgcccaggag ctgctgacca ccttcgaagc cgagatcggc
     2341 gaggttgcgc tgatcccggg aagcggaggg attttcgagg tgagggtgga tggcgagacc
     2401 gtctggtcgc gcaaggagcg gggaaggttt cccgagcttg ccgagctcaa acagctggtg
     2461 cgcgaccgcg tcgcccccgg caaggacctc ggccactccg aaaaaaaccg tgagcggtaa
     2521 gcagtaagca gtgagccggg aactcccagc tgcagaggcg tcccgtttta ccgcttaccg
     2581 cttaccgctt accgcttacc gcttaccgct taccgcttac cgtctaccgc ctatcaatac
     2641 cgtcgcgtga gcgtcatcgt cgcgccgtcg cgccgcatct cgacgcggtt gaggaagctc
     2701 aggccgagca ggccgagctc acccatgata ttgccctcga cgaccacgcc gtccacgttg
     2761 ttgagcgtga tgtcgccgag ccgcacggtg tcgagcttga cgcggtaggc gggggtggtg
     2821 ccgttggcgg tctggagcac cgctttctgg cccgcgaggt agttgatgcc cgcgcgcttg
     2881 gcgtcgccgc tgttgatggt cacgagcgag gcgccggtat ccaccatgaa cttcatcgaa
     2941 accccgttga tcgcagcggt ggtgacgaag tggccgcccg catcagcggt gagcgtggcg
     3001 cgctgggcgg tgacggggcc gccgccgagg gatatggtct gtcccatggt gagcgtctgg
     3061 cgcttgccgc cgatctcgaa cgtcgccgac tcgctcgtcg cctcaatcag cctgacgcct
     3121 tccggcgagg actgccccgc ctgcatcgtg aacggcttgc cgccgttgat cgtgaccacc
     3181 gccttgccgg cggtgagcgc cacgacgttg atatcggcag ccgcggcaag accggggaag
     3241 attaggaaaa gaaatacaat tgccctaaga ttgcgcactt ttgctcccct gcccgtgcaa
     3301 ggagaacagt gaaaccggtt gttatattcc gccacacgga gtccgagggt ccgggctatt
     3361 ttgccacata cctcacccgc cgcgacatcc cgtggcgggt gatcaggatc gacgagggcg
     3421 aagcggttcc cggaaatccg cgcgaattca gcgggctcgc tttcatgggc gggccgatga
     3481 gcgtcaacga cgatctgccc tggatcacgc ccgtgctgcg gctgatccgc tccgcggccg
     3541 acgccggtgt ttcgacgctg ggccactgcc ttggcggcca gttgatggcg aaggccttgg
     3601 gaggcgaggt cacgcgcaac ccggcgaagg aaatcgggtg gggaagggtg gacgtgctgc
     3661 agaacgacgt cgccggccgc tggtttggcg ccagtctgcg ttcgttcaat tcgttccatt
     3721 ggcacggcga gaccttttcc atcccccccg gtgcggtgcg gatcgcctcc agcccttact
     3781 gcgggaacca ggctttcgcg ctcggcccgc atctcggcat gcagtgccac gtcgagatga
     3841 cgccggagct cgtccgcacg tggtgccagg actgggaaca agaggtggag tcgcttgcgc
     3901 ggcgcatgcc ttccgtgcag acgccggcgg agatgatgga ggcggtcgag gaaaaggtcc
     3961 gaacgctcaa ttcaatcgcg gaccgtctct atgactgctg gactgctggc ctgaaactgg
     4021 gtgattaagt gatttagtga ctaggtgatt aagaaaattc tggtcttgtg actgcacact
     4081 tcgccgactt aatcactcaa tcacttagtc actgaattac ctttcctagt cgcggaagtt
     4141 cttgaattgc agcgggatgt cggtcactga cttccgcacc agcgcgatcg tctcctgcaa
     4201 cgcgtccttc ttgggtcccg agacccgcac ggcgtcgccc tggatacccg cctggacctt
     4261 gagcttgctg tccttgatga gcttgacgat ggccttgccg cgctcctgct cgatgccttc
     4321 gcggaccttg atcgtttcct tgaccttgtt gccgctgatc ggctcgatct tgccgggctc
     4381 gagcgcgcgg atgtccacgc cccgcttggc gagcctgccg gtgagcacgt cgcggagctg
     4441 gcgcagcttg aactcgtcgt cggcgaacgc ggtcagcgtc ttctccttgt cgttgatctc
     4501 gacgcgcgca tccgagccct tgaagtcgaa gcggttcgcg atttccttgt tgcactgctc
     4561 gatggcattg cggatctcga cgtggttgac ttcggaaacg atgtcgaatg acggcatgcc
     4621 ccctcctttc aacgccgcgg caattttacc gcaataagcc tctcaccgcg gagagatgga
     4681 acgaaaatct tgaaccacta aggacacaaa ggaaaaccaa gaacaataat tgaaccgcca
     4741 agccgccaag aaagacaaaa cagaaataag atcaaaatcc aatcgaaccg cagagcacgc
     4801 cgagagaagc aagacaaaat taaatcagga acgaaccgcc aagatcgcca agaagaatag
     4861 aagtctctct gccgcaggcc tagaacgcgc atcacaattg agctgtgggc gccgctcgct
     4921 accgagcgaa gtccgccgct cggtcccgcg aactggcccc ggcatgccag cctgccgctg
     4981 acctatcacc ggttcccgac atcgcctgac ctaccgtatg aaagaaacgc ctcgaaacag
     5041 ccggggtgcg gcaaagcgct tgccgtgctc ccccggttgg actagattgc tcggtgcgac
     5101 ccgtgatccc gctcacgacg ggagtccaag catgaggaat cttttcgttg ccaccgtcat
     5161 tgcagcgaca tggtgctctg gaacgctggc gcagacgcag tcctaccctg cgaagccggt
     5221 gcgcatcctg gtcggtttcg cgcccggcgg cggcatcgat atcgtcaccc gtctgtatgc
     5281 gcatcggctc acagaagtca tgggccagtc attcgtcgtc gaaaaccggc cgggcgcggg
     5341 cggcaccatc gcgaccgatc tggttgcaaa ggctccacct gacggccata cgctgatcgg
     5401 actgtcggtc acgcattcga tcaacgcctc gctctacagc aaactgccgt acgattcgat
     5461 caagaacttc acgccggtga gcccgaccgc gttgctggcg gatg
//
LOCUS       DBNGWZ010000554         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_960158, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000554 DBNGWZ010000000
VERSION     DBNGWZ010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_960158"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16CX_17225"
     CDS             complement(<1..851)
                     /locus_tag="AC16CX_17225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3014286.1"
                     /translation="MVKLKAISGVGGKGPACFLLETDRVRVLLDLGYGPNPGQWPDVG
                     GVGRVDALVLSHGHRDHAGALKLAPQVGNPPLYATSAVLARLGWNSKGTALPLQGEAE
                     VCGIEVRTGRDGHAPGGVWLHFSIGDGFLYTGDYGVESPIYAFDEPPAAGTVVLDASY
                     GDYSGSMTDCVARLSPVLERGAALLPVPPDGRGPELAYHIARSPAGPPCIGADLRASL
                     ERLASVELDSLRPGVAGELARIAREAPPIAEPNGMLLTGRADATDGEAAGLVARWEEA
                     AAPEIVFT"
     gene            complement(1009..1956)
                     /locus_tag="AC16CX_17230"
     CDS             complement(1009..1956)
                     /locus_tag="AC16CX_17230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014287.1"
                     /translation="MALIAVTSLAAGTVIAQQYPARPIRFIVPFPPGGGNDIVGRIVA
                     QKLSEGLGVSVVVDNRGGAGGTIGTDITSKAPPDGHTLLINNISLAVNATLFQKLPYD
                     TLRDLAPVSLLGRQPNVVVVNPSVPAASVKELLALARARPGQMTYGSGGIGTASHLAT
                     ELLKLMTKVDMIHVPYKGLGPALTDLMGGRLQLIISTLASALPQVKSGKLKPLAVTTA
                     QRSSFFPELPTMNEAGVPGYEFSTWYGLLVPGAAPKAIVERLNAETVKALNSPALKEQ
                     FTGQGLEATPSSTAEFGAHLKSEVAKWGKVIKAANIKVE"
     gene            complement(2204..2896)
                     /locus_tag="AC16CX_17235"
     CDS             complement(2204..2896)
                     /locus_tag="AC16CX_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3014288.1"
                     /translation="MEQDYLVILEHLLIALAAGGLIGFERSYHGRPAGFRTHTLVCVA
                     SSALMLLTVYQGHWFPEAFAGRVVLDPTRMAQGIMTGIGFLGAGVIFKEGLTVRGLTT
                     AASIWITAAIGILAGIGFYFPLIVTTLLTLGTLSLFRWIEAKVPGEFYAHLQVRYPKA
                     MVLAEEELRRFLADHGFSIANLSYASNAEGRYFEYRMVIRAHDAKQAQHLVESLSKAA
                     NVQEFRMSPAGD"
     gene            complement(3040..>3687)
                     /locus_tag="AC16CX_17240"
     CDS             complement(3040..>3687)
                     /locus_tag="AC16CX_17240"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3014289.1"
                     /translation="RGIGNAIGTYFIELARRDAERRELRLPDRDLAYFEEGAEHFDDY
                     VEAVGWAQGYAAANRRAMMDLVLAALRRHLPPFEVTSEVVNCHHNYVEREHHYGENVW
                     VTRKGAIRARAGDLGIIPGSMGTRSYIVRGKGSEESFQSCAHGAGRRMSRSQAQKTFT
                     PRDLAEQTTGVVCRKDRGVVDEIPAAYKPIDEVMANQSDLVEVVHTLKQVICVKG"
BASE COUNT          657 a         1213 c         1196 g          621 t
ORIGIN      
        1 gtaaagacga tctcgggcgc ggcagcctcc tcccagcgcg cgacaagacc ggccgcttcg
       61 ccgtccgtcg cgtcggcgcg tcccgtgagc agcattccgt tcggttccgc tatcggcggc
      121 gcttcacggg cgatgcgcgc cagctcgccg gccacgccgg ggcgcagact gtcgagctcg
      181 acgctcgcca ggcgttccag cgaggcgcgc aaatcagcac caatgcacgg tggtcccgcg
      241 ggtgagcgcg cgatgtgata agccagctcc ggcccgcggc catcgggcgg cacgggaagc
      301 agtgctgcgc cgcgttcgag gaccggggac aagcgcgcaa cgcaatccgt catggaaccg
      361 gaatagtcgc cgtaagacgc atcgagcacg accgtgccgg ctgcgggcgg ctcgtcgaat
      421 gcgtatatgg gcgactcaac cccatagtcg cccgtgtaca ggaatccgtc accaatgctg
      481 aaatgcagcc agacgccgcc tggtgcgtgg ccatcgcggc cggtgcggac ctcgatgccg
      541 caaacctccg cctcgccctg gagcggcagg gccgtgccct tactgttcca cccgaggcgg
      601 gcgagcactg ccgatgtagc gtacaggggg ggattgccga cctgcggcgc gagcttgagc
      661 gcgcccgcgt gatcgcggtg tccgtgcgac agcaccaagg cgtccacgcg ccccacgccg
      721 ccgacgtcag gccactggcc gggattgggc ccgtagccga ggtcgagcag cacgcgcacg
      781 cgatcggtct ccagcagaaa gcaggcggga ccttttccac ccacgccgga aatcgctttc
      841 agcttcacca tagcgatttc cgagagcgtg actcgtgact ggtgactggt gactggtgtc
      901 ctttcactcc caccctctcg acaagccttt ttcccaactt gggggaaaag aaaagcagcc
      961 ttgaagtcca gtcacgagcc gcgagccgcc ggttacctat caaggcggtc actcgacttt
     1021 gatgttggcc gccttgatga ccttgcccca tttcgccacc tcggacttga ggtgggcgcc
     1081 gaattccgcc gtggagctgg gcgtagcttc cagcccttgg ccggtgaatt gttccttcag
     1141 ggctggcgag ttcagcgcct taaccgtctc ggcgttcagc cgctcgacga tggcctttgg
     1201 cgccgcgccg ggcacgagaa gcccgtacca ggtgctgaat tcgtatccgg gcacgcctgc
     1261 ctcgttcatg gtcggcagct ccggaaagaa cgacgagcgc tgcgcggtgg taaccgcaag
     1321 cggcttgagc ttgccgctct tcacctgggg cagcgcggaa gccagggtcg agatgatgag
     1381 ctgcagccgt ccgcccatga ggtcggtcag cgccgggccg agacccttat acggcacgtg
     1441 gatcatgtcc acctttgtca tgagcttcaa cagctcggtc gcaaggtgcg aagccgtgcc
     1501 gatgccgccg gagccgtaag tcatctggcc gggccttgct cgcgcgagcg cgagcagctc
     1561 cttcaccgag gcggcgggca ccgaagggtt caccaccacg acattgggct ggcgtccgag
     1621 aaggcttacc ggcgcgaggt cgcgtaacgt gtcgtagggc agtttctgga acagcgtggc
     1681 gttgaccgcg aggctgatgt tgttgatgag cagggtgtgg ccgtcaggcg gcgccttgct
     1741 ggtgatgtcg gtgccgatcg tcccgccggc ccccccgcgg ttgtccacga ccaccgatac
     1801 gccgagcccc tcgctcagct tctgcgccac gatgcgcccg acgatgtcgt tgccgccgcc
     1861 cgggggaaat ggaacgatga atctgatcgg ccgcgcaggg tactgctgtg cgataaccgt
     1921 tcccgcggca agcgaggtca ccgcgatcaa cgccattgct acgcgctgca gaacgccgtg
     1981 ccaccaaccg ggcggcaagg ccccgtctcc gccccaaaac caaccccctc gccgagggcc
     2041 ggagcctcgc ccttgcggga acggaacgat gaagcgtatc ggacgggccg gaaaagcctg
     2101 cgccgccgca ggaaacgcct ggaccagcgt gagcgcgccg aagaccgcgg cggcaatcaa
     2161 ctttgaagtc gaatcacgat ccacgaatca cgactcaccg aatctaatcg ccggcgggcg
     2221 acatgcggaa ttcctgcacg ttcgcggcct tgctcaacga ttcgacaaga tgctgcgctt
     2281 gcttcgcgtc gtgcgcccgg atcaccatcc ggtactcgaa gtagcgcccc tccgcgttgg
     2341 aggcgtagct caggttggcg atcgaaaagc catggtccgc cagaaagcgg cgcagctcct
     2401 cctcggccag caccatggcc tttgggtagc gcacctgcag atgggcgtaa aactcgcccg
     2461 gcaccttggc ctcgatccag cggaacaggg aaagcgtgcc gagcgtgagc agcgtggtga
     2521 ctatcagcgg gaaatagaaa ccgattcccg cgaggatgcc gatggcggcg gtgatccaga
     2581 tggacgcagc ggtggtgagc ccgcggacgg tcagcccttc cttgaagatc acgccggccc
     2641 cgagaaagcc gataccggtc atgatacctt gcgccatgcg cgtcgggtcg aggacgacgc
     2701 gcccggcgaa cgcttccgga aaccagtggc cctgatatac cgtcagcagc atcagggcgc
     2761 tcgaagcaac gcataccagc gtatgggtgc ggaatcccgc cggccggccg tggtagctgc
     2821 gctcgaagcc gatcaggccg cccgcggcaa gcgcgatcag caggtgctcg aggatgacga
     2881 gatagtcctg ctccattcgt tccgtcctgc cagcctatag ggagagtcta acaaacctgc
     2941 gggacgccac cgtgagatgc ggaattcgag gcacagaatc cggatggaac gaatgaaaaa
     3001 ttactggtcc cgttcatgaa aaagcgcgaa gcgctttttt catcccttga cgcagatcac
     3061 ctgctttagc gtgtgcacca cttcgaccag atcgctctgg ttggccatca cctcatcgat
     3121 cggcttgtaa gccgcgggaa tctcgtcgac cacgccgcgg tccttgcggc acacgacgcc
     3181 cgtggtctgc tccgccagat cccgcggcgt gaacgtcttc tgcgcctgcg agcggctcat
     3241 gcgccgtccc gctccgtggg cgcacgattg gaagctctct tcagagccct tgccgcgcac
     3301 gatgtaggag cgcgtgccca tgctgccggg aatgatgccg aggtcgcccg cgcgcgcgcg
     3361 gatcgcgccc ttgcgggtca cccacacgtt ctctccatag tgatgctcgc gttcgacgta
     3421 gttgtggtgg cagttcacga cttcgctcgt gacctcgaac ggcggcaggt ggcggcgcag
     3481 cgcggcaaga accaggtcca tcatcgcgcg gcggttggcg gcggcgtagc cctgcgccca
     3541 gccgaccgct tcgacgtaat cgtcgaagtg ctcggcgcct tcctcgaaat aggcgagatc
     3601 gcggtcggga agccgcagct cgcggcgctc ggcgtcgcgg cgcgcgagct cgatgaaata
     3661 ggtgccgatg gcgttgccga tgccgcg
//
LOCUS       DBNGWZ010000555        12958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2094244, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000555 DBNGWZ010000000
VERSION     DBNGWZ010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2094244"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..5698
                     /gene="uvrA"
                     /locus_tag="AC16CX_17245"
                     /pseudo
     CDS             1..5698
                     /gene="uvrA"
                     /locus_tag="AC16CX_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            complement(5745..6314)
                     /locus_tag="AC16CX_17250"
     CDS             complement(5745..6314)
                     /locus_tag="AC16CX_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014290.1"
                     /translation="MRSFDQRNHAYPGFALFLRGTLDGAARDFSVGIGPSAQAKHAFR
                     AGDTVRGLAIPVADGRMETVELYKASALEVVSRAPAAPGGGPPWLGVPPSLETYRARG
                     HRRLDPRTYEASCRACIWGCRMPVEITLDQWNPEKREYRFETFCYGPKSCPAYRAGPT
                     RKVPGRSGMTWEEEDWVDEEATSNREPDE"
     gene            6463..7185
                     /locus_tag="AC16CX_17255"
     CDS             6463..7185
                     /locus_tag="AC16CX_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3014291.1"
                     /translation="MPRRARLRLAGFPLHIVQRGNNRTACFYADEDYALYLHQLGEMA
                     REFHCAVHAYVLMTNHVHLLLTPALPDGPSLLMKHLGQRYVQYVNRSYRRSGTLWEGR
                     FRSSIVQERGYFLRCHCYIELSPVRANMVSHPRDYRWSSFLVNAGLASSPMLTPHGEY
                     LALGRNDNERGAAYQELFRSELYPAQLEEIRSAVNGGFAFGNDRFKAQIAEMLKRRVE
                     RGAPGRPRKEKPKLSETGIQAG"
     gene            complement(7230..8222)
                     /locus_tag="AC16CX_17260"
     CDS             complement(7230..8222)
                     /locus_tag="AC16CX_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014292.1"
                     /translation="MNIRARAWCICSGVVWVVCVGVVSAQAPAPSGYPAKTVRMVVAF
                     APGGGTDVIARYFSQKFGESLGQSFVVDNRPGAGSNIGTDHVAKAAPDGYTLLMAIAS
                     MTINVTLYSKLSYDPVKDFTAIGTVAITPNCFAVHPSVPVKSMRELIALAKARPGEIS
                     YASPGSGTPVHLSVELFRSMAGIKLLHVPYNGSGPATVAVLGGQVPLLATTLPIGLPH
                     ARAGKLRMLGVTSAERTPLAPDFPTVAAASGLPGYEASVWYGLLAPAGTPAAVVNKLS
                     AEIERLLQLREVRERMATLGFDPYRRAPAAFAELIKTDIVKWGKVVRESGARAD"
     gene            8401..9378
                     /locus_tag="AC16CX_17265"
     CDS             8401..9378
                     /locus_tag="AC16CX_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014293.1"
                     /translation="MNRRAAVFFSFIGLAALSAAPVSADAQAYPAKPIRLIVPGTPGG
                     GSDTPARIMAHALGDDFGWRIVVDNRVGASGRIGTELVAKSPPDGYTLLLGSATPNAV
                     VPSAVSNLPYDAVNDFAPISLLATSDFILSVHPSLPVRSVRELIALARSKPGQINFAS
                     VGNISGAHVTGELFKQLAGINIVHVPYKGPGPAVTALISGEVSLYFASGPSVTPHAKA
                     GRLRLLATAGRQRSRMFPDLPTVGETVPGHEAYLWYGVMAPARTPKDIISALSAAILA
                     EGAKPRVIEQLAAVAMESVTNSPEEFAAFIKAEIAKWGKVVKASGTPVE"
     gene            9412..10314
                     /locus_tag="AC16CX_17270"
     CDS             9412..10314
                     /locus_tag="AC16CX_17270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3014294.1"
                     /translation="MKTILMTGAAGRIGTFLRPELAGNYRLRLSDIKPIRDLRPGETF
                     MRADLSKLSDMLRATQGVDAVVHFGGQSGEHDWDHILSANIIGFYNALEAARRNGVKR
                     FLVATSNHAVGFYRCDQTLDHRVYPRPDSRYGVSKVFNEALASLYADRYGMQMFCMRI
                     GNVNHAPIDRRRLAIWISGRDMAQLVTIGIEQPDLRFEIVYGISDNARAWFDNANAHR
                     LGYRPQDRSEPYAGEILKRDGPPGTSPADIYQGGGHCMSEGGTRSRLAGAPDAAAGTK
                     RIAARSKPAAGSTPAGSPRKRRRS"
     gene            complement(10370..11128)
                     /gene="modA"
                     /locus_tag="AC16CX_17275"
     CDS             complement(10370..11128)
                     /gene="modA"
                     /locus_tag="AC16CX_17275"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3014295.1"
                     /translation="MKSYAMSAAAIGLAVALAPAVAGAAEIKVISSVGVKGALEELQP
                     KFEGSTGHKLNITYGTAVPLKRQIDGGESFDVVILTPALVEDLAKAGKVAAGTSADVA
                     KAGIGVAIKRGSPKPDISTAEAFKKTVLAAKSIAYSKEGQSGTAMARLMERLDIADEV
                     RPKTVLETRPGMTAANVVEGKAELAFVLISEILPIAGAENAGPLPAGLQSYIVFTAGI
                     SPGAKDAKAAKSLIDFLKAPGAAPVYKAKGLEPG"
     gene            complement(11398..11571)
                     /locus_tag="AC16CX_17280"
     CDS             complement(11398..11571)
                     /locus_tag="AC16CX_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014296.1"
                     /translation="MLSIFVSTVAFFVSTYYAKRHLESMGVPKGATRGVLIFSIALVI
                     AFLVAFAVNRLPL"
     gene            complement(11917..12363)
                     /locus_tag="AC16CX_17285"
     CDS             complement(11917..12363)
                     /locus_tag="AC16CX_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3014297.1"
                     /translation="MRIFLDANILFSAAKSDGAIRRLLALLAEAGHECHADTYVVEEA
                     RRNLALKAPGNLPVLDRLLGRIHVAPFKAQLENAEVPGALAEKDRPVLAAAMQLGREA
                     LVTGDRTHFGAFYGKTLGGVKIHSPRSLAEAVLKPLRRKPPDAKNP"
     gene            complement(12373..12615)
                     /locus_tag="AC16CX_17290"
     CDS             complement(12373..12615)
                     /locus_tag="AC16CX_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3014298.1"
                     /translation="MKTTVTVTSRGVVTLPAKLRRTLGLKADDLLIAETTPEGLLLRP
                     AVTLPVEIYSEKRVREFDEAEAELEKVLPRQKKRAR"
BASE COUNT         2274 a         4370 c         4083 g         2231 t
ORIGIN      
        1 ttctcgaccg catggacaag ccgcaggtgg acgcgatcga gggcatcccg ccggcgatcg
       61 ccatcgacca ggtgaacccg gtgcgcacct cgcgctcgac ggtgggcacc atgacggagc
      121 tgaacgatca cctcaagctc ctctttgcgc gcgccgccgg cctctactgc cgcggctgcg
      181 ggcgccgcgt tgcgcgcgac acgcccgatt cgatccatgc cgactgcgcc gcgcgggcag
      241 aggccgcggg cgatccgcgc ctggtcatca ccttccccgt aacggtcccg aagaatttct
      301 ccgaagccga ggtgaagcag ctcctcgagc gccagggcta cacccgcatc cacgcgaaac
      361 agggtgacac gctcgaagtc gtccaggacc gtattcggat gtcgggcgca gcccgcgcgc
      421 gcgtgatcga agcgctggaa gccgcgctgc gcgtcggcca gggccgcgtc aacatccatc
      481 tggtcgccga gggcgacggg ggtcatccct catccttcat ccctcatccc tctccgtgga
      541 aatactccac ggaccttcat tgtccggatt gcgacatcca ctacgccgat cccacgccta
      601 gtctgttctc cttcaactcg ccgctcggcg catgcgggac ctgccgcggc ttcgggcgcg
      661 tcatcggcgt ggactacggc ctggtcgtcc cggatgaatc gaagactctg cgcgagggcg
      721 cgatccggcc gtggcagacc gagtcctatc gcggctggaa cgacgatctc gttcagtacg
      781 cgaaaaagcg cggcgtgccg ctggacacgc cgtggcgcga gctgacgggc gtacagcggc
      841 aatgggtgat cgagggcgat ccgggctggg tgagctggag gaagtcctgg cccggcctgt
      901 ggtacggcgt cgggcgcttc ttcaagtggc tggagaccaa ggcctacaag atgcacgtgc
      961 gcgtgctgtt gtcccgctac cgcgcctaca cgccgtgcgc ggcctgcggc ggcgcgcgac
     1021 tcaagaccga cgccctgctc tggcgtatcg gaagcaagga aaacgccgat gccacgctcg
     1081 agcccatggc gcgcttcaag ccgcagggcg tgaattggga cgcggagcgg cttgcgtcct
     1141 tcccgggcct ggcgctgcac gacctcatgc tgctgccgat cgaaaagatg cgcgccttct
     1201 tcgagaggct gcagcttccc tggacgtccg caacaaggac ttcgcaaatg aaggaggcct
     1261 ccggtgcgga cgtcccgatc gcctccaggg tcgcatcccc gaccaaggtc ggggtccctg
     1321 ctccgccctc cggcgctccc ctggacgaag cgaccgatct gctgctcacc ggcatccgcg
     1381 cgcggctcgg ctacctgacg gaggtcgggc tcggctatct caccctcgac cgccagtcgc
     1441 gcacgctatc cggcggggaa gtgcagcgca tcaacctcac cacggcgctc ggcacctcgc
     1501 tcaccaacac gctgttcgtg ctcgacgagc cctcgatcgg gctgcacccg cgcgacatgg
     1561 gccgcgtgat cggcgtcatg aagaagctgc gggacgccgg caactcgctg gtggtggtcg
     1621 aacacgatcc ccagatcatg ctcgccgccg accgcatact cgacctcggc ccgggcccgg
     1681 gcgaacgcgg cggcaaggtg gtgttcttcg gcgcgccgga ggaattgaag cgcgcgccgc
     1741 gctcgctcac cggcgactac ctctccggcc gcaagcgcgc ggacgcgcga ggcacgagtc
     1801 acgattcacg attcacgaat cacgccgttc agatccgcgg cgcctcggag cacaacctca
     1861 agaacatcga cgtgacgatc ccgctcgggc ggctggtgtg cgtcaccggc gtgtcgggct
     1921 cgggcaagtc cacgctgatg caggacgtgc tccacgccgc cctgctcaag gcaaagggaa
     1981 aaccgaccga agcccccggc cggcaccggg cgctgcgcgg tcaccagcac gtcgcggacg
     2041 tggtgctggt ggatcagtcg ccgatcgggc gcaccacgcg ctcgaacccc gcgagttacg
     2101 tcggcgcgtt cgaagcgatc cgccggctgt tcgccgcggc gcccgcggcg atcgagcgcc
     2161 gctataccgc gggaaccttc agcttcaatt cgggcaacgg ccgctgtccc gcctgcagcg
     2221 gcaacggctt cgagcacatc gagatgcagt tcctctccga cgtctacctg cgctgcccgg
     2281 actgcaacgg caggcgctat cggccggagg tgctcgaagt gaagctcttg cctccggccg
     2341 cgacccgagt cacgagtcac gagtcatagg aaagtccatc gcggatgtgc tggacatgac
     2401 cgtaacggag gcgattgatt tcttcagcga gaatcctgaa gtgcggcgcg cgctgaaacc
     2461 gctcgcggac gtcgggctcg actacctgcg cctgggccag ccggtgccga cgctgtccgg
     2521 cggcgaggcc cagcgcctga aactcgctgg ccatctcgcc gaggccgcgg gccgtagggg
     2581 ttcctcctca cgcctcacgc ctcacgcctc actattcttg ttcgacgagc cgaccaccgg
     2641 cctgcacttc gacgacgtcg ccaaattgct cggcgcgttc cgtcagctgc tcgccgccgg
     2701 gcactcgctg gtcgtcatcg agcacaacct cgacgtcatc cgctccgccg actggatcat
     2761 cgacctcggc cccgagggcg gcgacgccgg cggcgaggtc gtcgcgatcg gcacgccgcg
     2821 ggagattgcc gcgcacccga cctcccacac cgggaaagcc ctgcgcgaat acgaatccgc
     2881 actggtgcca gcggcggcag ccggggagcc gctcgctaga gcgcacatca cgagtcacga
     2941 gtcacgagtc acgaatcacg ctattcagat acacggcgca cgtgaacaca acctgaagaa
     3001 catcgacgtc gcgattccgc gcaaccgctt cacggtggtg accggggtct ccggcagcgg
     3061 caagtccacg ctcgccttcg acatcgtgtt cgcggagggc cagcgccgct acctcgaatc
     3121 gctcaacgcc tacgcgcggc agttcgtcga gcccgcggcg cggcccgacg tggacgcgat
     3181 cttcggcatc ccgccgacgg tcgcgatcga gcagcgcacg agccgcggcg ggcgcaagag
     3241 cacggtggca acgttgaccg agatccacca cttcctgcgg ctgctctaca tgaagctcgg
     3301 cacccagcat tgcccgaact gcgacgtggc gatcgagccg cagagcgtgg acgcgatcgc
     3361 ggcgcgcatc gcgcgcgacc accgcggcgc gcgcgtggga ttgctcgcgc cgctggtagt
     3421 gaaccgcaaa ggctattaca ccgacctcgc caagtgggcg gccggccgcg gctacaccca
     3481 cctgcgcgtg gaccgcgagt tcataccgac cgccaactgg ccgcggctgt cgcgcttcca
     3541 cgagcacacc atcgagctgc cggtcgccga cgtgcgcgtc acgccggaca acgaagcggc
     3601 gctgcgggcg gcgctccacc gctccctcga tcacggcaag ggcgtcgtcc acgtcctcgc
     3661 gccgctggag gcgctcgagc gcgcgatggc gaggcgcgac gccgcggctg cccggctgac
     3721 ggaaacggtc ttttccgtaa agcgcgcctg cccctcgtgc ggcaccagct ttcccgagct
     3781 cgatccccgg ctgttttcct acaactcccg gcacggctgg tgcgagagct gctacggcac
     3841 ggggttgagg ctcgccggct tcgacgagga gcagagcggg gaggagatct ggtggaacga
     3901 atggtacgaa ggggagccga ttccctgcgc ttcgtgcggc gggaatcggc tgaatccgat
     3961 cgcactggcg gtgaagttcc gtgggctttc cattgccgat ctgagtcgga tgtcggtgtc
     4021 ggatatcgag aagtttttcg ttaatctccg cttggaggga cgacaagcgg agatcgcgcg
     4081 cgacatcgtc gccgagctga agagccgcct cgccttcctg cgcgaggtgg ggctgtcgta
     4141 tctcgcgctc gaccgcgccg cgccgacgct ctccggcggc gaggcgcagc gcatccggct
     4201 cgcggcgcaa ctgggctcca acctgcgcgg cgtcgcctat atcctcgacg agcccaccat
     4261 cgggctgcac ccgcgcgaca accgcatcct gctcgacacg ctcgggaagc tcgaggccaa
     4321 gggcaacacc ctgatcgtgg tcgagcacga cgaagagacg atccgccgcg cggaacacgt
     4381 gattgacctc ggcccgggtg cgggcaaccg cggcggcgag atcgtcgcgg tgggggccgc
     4441 cgccgacttg atgcgcgttt ccgcctcggt caccggccgc ctcctcgcgc agccgctcaa
     4501 gcacccgctg catgcgcggc gccccgtgaa cggcgcgact cccgctctcg ccatcgagca
     4561 cgcgcgcctg cacaacctga aggatctcga catccgcgtg ccgttcggca ggctcatcgt
     4621 ggtgaccggc gtctcgggct cgggcaagtc caccctcgcg cgcgacgtgc tctacgcaaa
     4681 cctgcggcgt atgctcgctg ctggcgccat gcgccgcaag gcgaaagtcg aaagtcgaaa
     4741 gtcgaaagga aaatcgaaca aacgcgagtc tacttccgcc tttcgccttt ctccttccgc
     4801 cttcgacccg gtcggctgcc gcgccataag tggcgcggag cggatcgatc gggtcctgga
     4861 ggtggaccag acgccgatcg gcaagacccc gcgctcctgc cccgccacct acgtcggctt
     4921 ctgggaccag gtgcggcgac tctacgcgga ggtgaccgaa tcgcgcatgc gcggctatac
     4981 cgcctcccgc ttctccttca acacggcggg cgggcgctgc gagacctgcg agggacaggg
     5041 cgtgaagcgc atcgagatga gtttcctgcc cgacgtgaag gtggcgtgcg aggcgtgccg
     5101 cggcgcgcgc ttcaaccctg agacgctcgc ggtgcggttc aaggacaaga ccatcggcga
     5161 ggtgctcgcc atgagcgtgg acgaggcggc cgggttcttc tccgcccatc cctccatcca
     5221 ccatgcgctc acgttgttgc aggacgtcgg gctcggctac ctcaccctcg gccagcagag
     5281 cccgacgctc tccggcggcg aggcgcagcg catcaagctc gtcaccgaac tcgcgaaagt
     5341 gcgaaacaat gctcccatca cccatcactc atcacccatc acgctttatg tgctcgacga
     5401 gcccaccgtc ggcctgcaca tggcggacgt ggagaagctc gtgcgcgtgc tgcaccggct
     5461 ggtggacgcc ggcaacaccg tgatggtgat cgagcacaat ctcgacgtca tcgccgaggc
     5521 cgactgggtc ctcgacctcg ggcccgaagg gggcgatgcc ggcggccgca tcgttgcgca
     5581 aggcgcgccg gaggcggtag cgaagaacgg cgcgcgctcc cataccgcgc agatcctcgc
     5641 tgacttcctg cgcgagcgcg gcgtcaaaaa cgaatcagcc gccgatgaac gccgataaga
     5701 accaaaaccg gcaacggcaa aaccggggac agaccacgtt tttcctactc atccggttcg
     5761 cgattcgagg tcgcttcctc gtccacccaa tcctcctctt cccatgtcat gccgcttcgc
     5821 ccgggcactt ttcgtgtcgg gccagcgcga tacgccggac aggatttcgg tccgtaacaa
     5881 aacgtctcga accggtactc acgcttctcg ggattccatt gatccaaggt gatttctacc
     5941 ggcatgcggc aaccccagat gcaggcacgg cacgacgcct catacgtgcg tgggtcgagc
     6001 ctgcggtgac cccgcgcgcg ataggtctcc agggacgggg gcacgcctag ccagggcggt
     6061 cccccgccag gtgccgccgg ggcacgcgaa acgacttcga gagcgctcgc tttgtagagc
     6121 tcgaccgtct ccatgcggcc atcggcaacc gggatcgcga gcccgcgcac ggtatccccc
     6181 gctctgaacg cgtgcttcgc ttgcgcactc gggccgatgc ccaccgaaaa atcccgcgct
     6241 gcgccgtcca gtgttccgcg tagaaacaac gcaaacccgg gataggcatg gttgcgctgg
     6301 tcgaatgagc gcatgagccg gatccgcggc tgaaccgcga ccagctcccc cgaccaggtc
     6361 agcttggtaa ccggggacgg accacgtttt ctggcctcgc tgtcttccgg agccacggtt
     6421 ggcgcgttga tacccagtaa caagcagaga tgctaaccca tcatgccccg ccgtgcgcgc
     6481 ctgagattgg ctggcttccc cctgcacatc gtccagcggg gcaataaccg caccgcctgc
     6541 ttctacgcgg acgaggacta cgccctctac ctccatcagc tcggggaaat ggcgcgggag
     6601 ttccactgcg ccgtgcacgc gtacgtcctg atgaccaacc acgtgcacct gctgctgaca
     6661 ccggcgcttc ccgacgggcc ctcgctactg atgaagcacc tcggccagcg ctacgtgcag
     6721 tacgtaaacc gctcctaccg gcgcagcggt acgttatggg aggggcggtt tcgctcgagc
     6781 atcgtgcagg agcgcggata ttttctgcgc tgccactgct acatcgaact cagtcccgtg
     6841 cgcgccaaca tggtttccca cccccgggac tatcgctggt ccagcttcct ggtcaatgcc
     6901 gggcttgcct cctcgccgat gctgaccccg cacggcgaat acctcgcgct gggacgaaac
     6961 gataacgagc gaggcgcggc ctaccaggaa ctcttccgct cggagctgta tcccgctcag
     7021 ctcgaagaaa tccgctcagc ggtgaacggc ggcttcgcgt tcggcaacga tcggttcaaa
     7081 gcgcaaatcg cggagatgct caagcgccgc gtggagcgcg gcgccccggg acggccaagg
     7141 aaggaaaagc ctaagctatc agagacgggt attcaggccg gctaatttca tctgccggaa
     7201 aacgtggtct gtcccctaat acgcgcaggc taatcggccc tggcgccgga ctcgcgcacg
     7261 accttgcccc atttcacgat gtccgttttg atcagctccg cgaacgcggc cggcgcgcgg
     7321 cgataaggat cgaagcccag cgtcgccatc cgctcgcgca cctcgcgcag ttgcagcagc
     7381 cgttcgatct ccgcgctcag cttgttgacg accgccgcgg gcgtgccggc cggcgccagc
     7441 aaaccgtacc acacgctcgc ctcgtaaccc ggcaatcccg aagccgcggc gacggtcggg
     7501 aaatccggcg cgagcggggt tcgctccgcg ctggtcacgc cgagcatcct gagcttgccg
     7561 gcccgcgcgt gcggcagccc gatcggcagg gtggtcgcga gcagcggaac ctggcccccg
     7621 agcactgcaa cggtggccgg gccgctgccg ttatagggca cgtgcagcag cttgatcccg
     7681 gccatcgacc ggaacaattc caccgaaaga tgcaccggcg tgccgctgcc gggggacgcg
     7741 tagctgatct ccccgggcct cgccttcgcc agagcgatca gctcgcgcat cgatttcacc
     7801 ggcacggacg ggtgaacggc gaagcagttc ggcgtgattg cgaccgtgcc gattgccgta
     7861 aagtccttga ccgggtcgta ggagagtttg ctgtagaggg tgacgttgat ggtcatggaa
     7921 gcaatcgcca tcaacagcgt gtagccgtca ggggccgcct tggcgacatg gtcggtgccg
     7981 atgttgctgc ccgcgcccgg acgattgtcg acgacaaatg actgccccag cgattcgccg
     8041 aacttttgcg agaaatagcg cgcgatcacg tcggtccctc cacccggagc gaaggcgacg
     8101 accatgcgaa ccgtcttggc gggatagccc gacggcgccg gagcctgggc tgatacgaca
     8161 cccacgcaca ctacccaaac aacaccgctg cagatgcacc atgcccgggc gcggatattc
     8221 attggacgac tccgtttcca aaagtgaacc atggtgatca aatccaaaca aaaaggatcg
     8281 actcaccccg gccctattta ttctagacta agaacgtttc agtcacccgt atcctgtcac
     8341 aagagtttgg gacctcatgc tggaccagca tcaagcggtc attgcgagga gaaacaggga
     8401 atgaaccgga gagcggcagt atttttttcg tttatcggcc ttgccgcgct gtcggccgcc
     8461 cctgtttccg cggacgcaca ggcctatccg gcgaaaccga tccggctgat cgtgcccggc
     8521 accccgggtg gggggtcgga tacgccggca cgtatcatgg cgcacgcgct cggtgatgat
     8581 ttcggctggc ggatcgtggt ggacaaccgc gttggcgcaa gcggccgcat tggaaccgag
     8641 ctcgtggcaa agtcgccccc cgacggctat accctcctgt tgggaagcgc gacgccaaac
     8701 gcggtcgtcc caagcgccgt ctcgaacctg ccatacgacg cggtgaacga ctttgcgccc
     8761 ataagcctcc tcgcaacctc cgatttcatc ctctcggtgc atccctcgct gccggtgcgc
     8821 tcggtccggg agctgattgc gctggccagg agcaagcccg gacagatcaa tttcgcttcc
     8881 gtcggcaata tttcgggggc acacgtcacg ggcgaactgt tcaagcagct cgccgggatc
     8941 aacatcgtcc atgttccgta caagggtccc gggcctgccg tcaccgcgct catcagcgga
     9001 gaagtttctc tttactttgc cagcgggccc agcgtgacac cgcacgcgaa ggccggacgg
     9061 ctgaggctgc tcgcgaccgc cggtcggcaa cgctcgagaa tgttcccgga tcttcccacg
     9121 gtcggcgaga cggtgcctgg ccacgaggcg tatttgtggt acggcgtcat ggctcccgcg
     9181 cggactccga aggacatcat ctcggcgttg agcgccgcaa tcctcgccga aggcgcgaag
     9241 cccagggtaa tcgagcaact cgctgccgtc gccatggaat cggtcactaa ctcgccggag
     9301 gaattcgccg cgttcatcaa ggcggaaatc gccaagtggg gcaaggtggt gaaggcatcc
     9361 ggcacgccgg ttgaatgagc tgaccccatt tgttccttgg gagggcattg catgaagacg
     9421 atcctgatga cgggtgccgc cgggcgcatc ggcacttttc tgcggcctga gttggccgga
     9481 aactaccgac tccggctgtc cgacataaag ccgatccgcg atctgcgccc cggcgagacc
     9541 ttcatgcgcg cggacctctc caagctatcc gacatgctgc gggccaccca gggcgtggat
     9601 gccgtggtgc acttcggcgg gcagtccggc gaacacgatt gggatcacat tctctcggcc
     9661 aatatcatcg gtttctacaa cgcgctcgaa gccgctcgca ggaacggggt caagcgattt
     9721 ctggtcgcga cttcgaatca cgcggtcggc ttctatcgct gcgatcagac cctcgaccac
     9781 cgcgtctatc ccaggcccga cagccgctat ggcgtgtcca aggtcttcaa cgaggcgctc
     9841 gccagcctgt acgccgaccg ctacggcatg cagatgttct gcatgcgcat aggcaacgtg
     9901 aatcacgctc cgattgaccg gcgccggctc gccatctgga tcagcgggcg cgacatggca
     9961 caactggtca ccatcggcat cgaacaaccg gatctgcgct tcgagatcgt ctatggcatt
    10021 tcggataacg ctcgcgcctg gttcgacaac gccaatgcac atcgcctcgg ctaccggccg
    10081 caggatcgga gcgagccgta cgccggggaa atcctgaagc gggacggtcc cccgggaaca
    10141 tcgcccgcgg acatctatca aggagggggc cattgcatgt ccgaaggcgg aacccgcagc
    10201 cgactcgcgg gcgcgcccga cgcagcagcc gggacgaaac gcatcgcggc ccgatcgaaa
    10261 ccggcggccg gaagcacgcc cgccggttcc ccccgcaagc ggcgccgatc atgagaatcg
    10321 cgctcgggct ttgaccggcg ctcgcgcgtt caatgcaggc gcgagggtat caacccggct
    10381 ccaggccctt ggccttatag accggggctg cgcccggcgc cttgaggaaa tcgatgaggc
    10441 tcttggcggc tttcgcgtcc ttcgcgccgg ggctgatgcc ggcggtgaac acgatatagc
    10501 tctgcaaccc ggccggcagt ggacctgcgt tctccgcccc cgcgataggg aggatttcgc
    10561 tgatcagcac gaatgcaagc tcggccttgc cctcgaccac gttcgcggcg gtcatgccgg
    10621 gacgggtttc cagcacggtc ttcggtcgca cttcatcggc gatgtcaagt cgttccatca
    10681 ggcgcgccat ggcggtgccg ctctggccct ccttggaata ggcaatcgac ttggcggcga
    10741 ggacagtctt cttgaacgct tcagccgtgc tgatgtcggg cttgggcgag ccccgtttga
    10801 tggcgacgcc gatgccggcc ttggcaacgt ccgctgacgt gccggcggcg actttgcccg
    10861 ccttggcgag atcttcgacc agcgccgggg taaggataac gacgtcgaag ctctcgccgc
    10921 cgtcgatctg ccgcttcaat ggcactgcgg tgccgtaggt gatgttgagc ttgtgtccgg
    10981 tcgagccctc gaacttcggc tggagctcct ccagcgctcc cttcacgccg acggagctga
    11041 tgaccttgat ttcggctgcg ccggcgacag cgggtgccag cgcgacggcg agcccgatcg
    11101 cggcggcgga cattgcgtat gacttcatgg cgatctccct gttccgctcc ggtgcacgag
    11161 cgattttggc ggccatcgga tctcatcgcg ttcatgccgc tgcacggtca cgcgcctttg
    11221 acaccgatgg ttgccggaca gcttagcacc ggtcgctcca ggtccaaagg tccgatgagc
    11281 ggtagcaccc ttgcttcttt caatgggtct gtccccattg atttggttta ttttgtagca
    11341 cggcgttgat tccgcgtagc cgatgaacta cagatggagt gatgaagttc agcaggttca
    11401 gagcggcagc cgattcaccg cgaacgccac caggaaggcg atcaccagcg cgatggagaa
    11461 gatcagcacg ccccgcgtcg cgcctttcgg gacgcccatg ctctcgaggt ggcgcttggc
    11521 gtagtaggtc gagacgaaaa aagcgacggt cgatacgaag atgctcagca tcgggatttc
    11581 ccgaggttcg agccgttcat tctaatccgc cttcgggtta tgccctcatc cctcgggcga
    11641 tcccgtcagt cgtcactcgt cagtcgtcac tggggttaga agcattctgt cttttctgcc
    11701 ttcatccctg cccttgggcg tgcaccgcgc cgctgatcac ccgtcactcg tcactcaaag
    11761 taggggtaga caaacgggct gtggaaaagc gcgttaactc actgaaacgt tgcttcgttc
    11821 aatggggtct gtcctttgtt ctcgaaacgg ctactccttc cgcctttcga ctttcgactt
    11881 ccgacttggg tcttagcgtt ttcggggggt ctgaccctat ggatttttcg cgtctggcgg
    11941 cttcctgcgc aggggtttca gcaccgcctc cgccagcgat cgcggcgaat ggatcttcac
    12001 gccaccgagc gtcttgccat agaaggcacc gaagtgcgtg cggtctcccg tgaccagcgc
    12061 ctcgcggccg agctgcatcg ctgcggccag cacgggccga tctttttcgg caagagcccc
    12121 gggcacctcg gcgttctcca gctgtgcctt gaacggggcc acgtggattc gccccagcag
    12181 ccgatccagg accgggagat tcccgggcgc cttcagcgca aggtttcggc gcgcctcctc
    12241 aacgacatag gtatcggcat ggcactcgtg cccggcctcg gcaagaagcg cgagcaatcg
    12301 ccggatggcg ccgtcggact tggcggcgga gaaaaggatg ttcgcgtcga ggaaaatccg
    12361 catccgcggc ggtcagcggg cgcgcttctt ctggcgaggc aggacttttt cgagctcagc
    12421 ctccgcttcg tcgaactcgc gaacgcgctt ctcgctgtag atctcgacgg ggagcgtaac
    12481 ggccgggcgc agcagcaggc cttccggcgt ggtctcggcg atgagcagat cgtccgcctt
    12541 gaggcccagg gtccggcgga gcttggccgg cagcgtgacg acgccccggc tggtcacggt
    12601 cacggtggtc ttcatggcgg cagaatagca gaaatacagt aatccggcaa ttcagggcga
    12661 catgccttaa agcgcgtgaa tcacccccga aaaagggccg ctaagcgcga aaaaggcttc
    12721 gattcggcgt tcttgtaatg cagaatcaat ctgttgcgat ggtccgaccc ggattgtgtg
    12781 tgctgacggt catccgttgt ggctcataac tcgaaccgaa aatccgggag atcgggagat
    12841 cgggaggtcg ggagatcgtg agatcgaatg cgcgggaggt cgtgagatcg agaagtcgga
    12901 agatggaaga aattcagtat ccctctccag ccctcacccc cacccccccc ccccgggt
//
LOCUS       DBNGWZ010000556         6830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1591975, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000556 DBNGWZ010000000
VERSION     DBNGWZ010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1591975"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16CX_17295"
     CDS             <1..464
                     /locus_tag="AC16CX_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3014299.1"
                     /translation="ERLMQGFQPKAGRYAIQYKSIYKVHQRVASDFRLGRALLAGDAA
                     HLNNPIGAFGLNGGIHDAINLAGKLGKVCRGESGERLLDLYVRQRRTVNLEYVQEHSI
                     RNLERLTARTEEERRRNFDELRRAASTVKGARNFLLISSMIASVRRANAIT"
     gene            682..2418
                     /locus_tag="AC16CX_17300"
     CDS             682..2418
                     /locus_tag="AC16CX_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR3014300.1"
                     /translation="MDKAPPVTLHSTAHYFLEGLNELGIEYLFCNFGTDHAPLIEEMA
                     RWQAEGRKLPQTLLCPHENTAVQMAAGYAMVTGRGQVVMVHVDAGTSNAAMGLHDACC
                     SRVPLLLLAGKAPYTVRGELPGSRDHYVHFIQEPFDQGAIVRNYVKWEWTLPSGIVTK
                     EALRRAHTIAHSDPRGPVYLMLSREMLAQTWDTAAVRSFPEERYGAASAGAAGADEIA
                     ALAERLLAAKHPILITAYAGRNRRAPEVIEALARFAGIRVFEFNPVYLNLSRASPCWG
                     GLQPGRHVTDADVGLLVDVDVPWVPKETRENPQTWWAHIDVDVVKERFPIWGFPSNLR
                     LPGDSVLILSQVLDALRTKATPAFRDAAAKRLEAMAREHGGHRAELAKLAANKGAAGA
                     VNPHYLCAELAKALGEDDIVVNESIRNMGVVFNQIPRTRPGTSLGSGGAGLGCSAGMA
                     LGAKLARPEATVVQVVGDGSFYLGNPSSAYAVSKQYRLPIFTVVLDNSGWSAVKESTL
                     RMYPDGAAKALNEFGARLAPDIEFAKVCEAAGGHGETLAEPGAAPAAIRRCLEAVRGG
                     RPAVLHAKIPVL"
     gene            2521..3486
                     /locus_tag="AC16CX_17305"
     CDS             2521..3486
                     /locus_tag="AC16CX_17305"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014301.1"
                     /translation="MRTAFFNLAAGLMLCIAADSLPAQAYPTKPIRIIVGYAAGGGND
                     IIVRVLVPKLSEALGQPVIVDNRPGAQSIIAAEMVAKATPDGYTLLMGPSGPMTMNPA
                     IYSKLPYSPLRDFAPITMIGSFPLILVVSPSLPVKTVKELVAYAKANSDKVSYGASAA
                     AFQLTTELFKQRTDTRFIHVPYKGVNESVNAVMAGQVTMTIGDPAVIVGLMKAGRVRA
                     LAVTSATRHPVWPELPTLSEAGVPDIEVVLWTGFHAPVRTPAAAIRKFREEVVRVIRL
                     PEIRERLAAMAIEPSGNAPEEYKRIIASDIKKWTAVAKAANIKAD"
     gene            3622..4458
                     /locus_tag="AC16CX_17310"
     CDS             3622..4458
                     /locus_tag="AC16CX_17310"
                     /EC_number="1.13.11.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gallate dioxygenase"
                     /protein_id="HIR3014302.1"
                     /translation="MARIIGGIGTSHVPTIAMAYDRGKRNDPDWAPLFKGYEPVAAWL
                     AARRPDVLVFFYNDHATTFFFDHYPTFALGVSAEYPIADEGLGPRPVPPLKGHAPLAR
                     HLAESLVNEEFDISVFQDLPIDHGCHSPLTMFWPPAPDWPGSIVPIEVNVLQHPLPTP
                     MRCYKLGQAVRRAVESYPGDLKVVVVGTGGLSHQLNGERAGFNNPEWDARFLDLIERD
                     PGALAAMRHADYIRLGGTEGAEEIMWLAMRGALSDRARKIHQNCYLPMTTAMAVALFE
                     EP"
     gene            4520..4660
                     /locus_tag="AC16CX_17315"
     CDS             4520..4660
                     /locus_tag="AC16CX_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014303.1"
                     /translation="MYSDRDGAIRGYDPVAYSHGVRERRKKDVPGHIRKGDANWPAVL
                     QR"
     gene            4778..4957
                     /locus_tag="AC16CX_17320"
     CDS             4778..4957
                     /locus_tag="AC16CX_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014304.1"
                     /translation="MRCHHCGKLTVVRTLGKGEEFLHRCRHCNGFHKFTSAGRHGSGA
                     INYRVTKVLPAPASK"
     gene            5059..5265
                     /locus_tag="AC16CX_17325"
     CDS             5059..5265
                     /locus_tag="AC16CX_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3014305.1"
                     /translation="MPIGTVKWFNPAKGYGFIQPDDGSKDVFVHISAVERSGLGTLKE
                     GQKVQFDLARGKDGKTSADNLKSA"
     gene            5469..6227
                     /locus_tag="AC16CX_17330"
     CDS             5469..6227
                     /locus_tag="AC16CX_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014306.1"
                     /translation="MTKRADIPVITHCAGPHDGHISWQRMLSSAVGAAALITATTASA
                     AGPSAEGDVYVYRLVNGYNKEVVGQLRYQVSKVERGNVTFSVTPDNPSAGVERTEIFT
                     KEGNWLRHLLENHGQKVEYEFAAAYPAYVFPLEPGKSWSLRVSATVPTTGQRRSVRVD
                     GRVIGNERIRVPAGEFDTVKIRRLVYPGDADYYLPETHIIEFDWYAPALGRTVRTERK
                     SDYLDLTRCHEESSCVYHGDWDVFELVDIRALKP"
     gene            6614..>6830
                     /locus_tag="AC16CX_17335"
     CDS             6614..>6830
                     /locus_tag="AC16CX_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014307.1"
                     /translation="MNAANAQQYPSRPIRIVVPFPPGGASDVTARMLADKFASSWGAN
                     VVVDNRTGASGIIGTDIVAKAAPDGYTL"
BASE COUNT         1247 a         2207 c         2248 g         1128 t
ORIGIN      
        1 tcgagcggct gatgcagggc ttccagccaa aggcgggacg ctacgccatc cagtacaaga
       61 gcatctacaa agtgcaccag cgcgtcgcca gcgatttccg cctgggtcgc gcgctgctcg
      121 ccggagatgc cgcgcacctc aacaatccga tcggcgcgtt cgggctgaac ggcggcatcc
      181 acgatgcgat caacctcgcc ggcaagctcg gcaaggtatg ccgcggcgag agcggggagc
      241 gcctgctcga tctctacgtg cgccagcgcc gcacggtgaa cctcgaatac gtccaggaac
      301 actcgatccg gaacctggag cgcctcaccg ccaggaccga ggaggaacgc cgcaggaact
      361 tcgatgagct gcgccgggcc gcgtcaacgg tgaaaggcgc gcgcaacttc ctgctgatct
      421 ccagcatgat cgcgagcgtc cggcgcgcga atgccataac ttaaggggtg agccggggtt
      481 tcgaaaaatg tgcctgcgca catttgagcc gacgaacgcc cgacgcgatg cagcgcgtcg
      541 ggccggagga gggcggagcg gcgaaggccg cgttgagttc cattgctatc cacctctgcc
      601 gcgccaggaa tcgtctatca taagctgcac gacccgcgtt tagccgtccg tgggggaaac
      661 aaatcctcgg agaaattcga tatggacaaa gctccgccgg tcaccctgca ctcgaccgcg
      721 cactactttc tcgaagggct gaacgagctc ggcatcgagt acctgttctg caatttcggc
      781 accgaccatg ctcccctcat cgaggagatg gcgcgctggc aggccgaggg acgcaagctg
      841 ccgcaaacgc tgctctgccc gcacgagaac accgcggtgc agatggccgc aggctatgcg
      901 atggtgacgg gccgcggaca ggtggtgatg gtgcacgtgg acgcgggcac ctcgaacgcg
      961 gcgatgggcc tgcacgacgc ctgctgcagc cgcgtcccgc tgctcctgct cgcgggcaag
     1021 gcgccctaca cggtgcgcgg cgagctgccc ggctcgcgcg accactacgt ccatttcatc
     1081 caggagccgt tcgaccaggg cgcgatcgtg cgcaactacg tgaagtggga atggacgctg
     1141 ccctccggca tcgtcaccaa ggaagcgctg cggcgcgccc ataccatcgc gcacagcgac
     1201 ccgcggggcc ccgtatacct gatgctgtcg cgcgaaatgc tcgcccagac ctgggacacg
     1261 gcggcggtgc gctcgtttcc cgaagagcgc tatggcgcgg ccagcgccgg cgcggctgga
     1321 gcggacgaga ttgccgccct cgctgagcgg ctgctggccg cgaaacaccc gatcctgatc
     1381 acggcgtacg cgggacgcaa ccgccgcgcg ccggaggtga tcgaggcgct cgcgcgcttc
     1441 gccggcatcc gcgtgttcga gttcaacccc gtttacctca acctctcgcg cgcttcgccg
     1501 tgctggggcg ggctccagcc cggccggcac gtcacggacg ccgacgtggg gctgctggtg
     1561 gacgtggatg tgccctgggt tccgaaggaa acccgcgaga acccgcagac ctggtgggcg
     1621 cacatcgacg tcgacgtcgt caaggagcgc ttcccgatct ggggatttcc ctcgaacctg
     1681 cgcctgcccg gcgacagcgt gctgatcctg tcgcaggtcc tcgacgcgct gcggacaaag
     1741 gcgaccccgg cgttccggga cgcggcggcg aagcgtctcg aggcgatggc gcgcgaacac
     1801 ggcgggcacc gcgccgagtt ggcaaagctc gctgcgaaca aaggcgcagc gggcgccgtg
     1861 aatccgcatt atctctgcgc cgagcttgcc aaagcccttg gcgaggacga catcgttgtc
     1921 aacgaatcga tacgcaacat gggcgtggta ttcaaccaga ttccgcggac gcggcccggc
     1981 acctcgctgg ggagcggcgg cgcgggcctc ggctgttcgg ccggcatggc gctcggcgcc
     2041 aagctcgccc gccccgaagc gacggtggtg caggtggtcg gggacggcag cttctacctc
     2101 gggaatccct cctcggcgta cgccgtctcg aaacagtacc gactcccgat attcaccgtc
     2161 gtgctcgata attcgggctg gtcggcggtg aaggaatcga cgctgcgtat gtacccggat
     2221 ggcgcggcga aggcgctcaa cgagttcggg gcgcggctcg cgcccgacat cgagttcgcc
     2281 aaagtgtgcg aggcggcggg cggccacggc gaaacgctgg cggagcccgg cgcggcgccg
     2341 gcggcgatcc ggcgctgtct cgaagcggta cgcggtggcc gcccggcggt gctgcacgcg
     2401 aagatcccag ttctctgaat cgaggcgacc caacattcga gtaaaggttc catttacgcc
     2461 gtgcttctgt ccgagcttat aacgcgaaat agagtcgcca cgattacaac aggaggctgc
     2521 atgagaacgg cgttcttcaa tttggcggca gggctgatgc tgtgcatcgc cgccgattcg
     2581 ctgccggcgc aggcctatcc gaccaaaccc atccgcatca tcgtcggcta cgccgcgggc
     2641 ggcggcaacg acatcatcgt gcgggtcctc gtccccaagc tgtcggaagc gctgggccag
     2701 ccggtgatcg tggacaaccg gcctggcgcg cagtccatca tcgccgcgga aatggtcgcg
     2761 aaggcgacgc ccgacggcta cacgctgctg atgggtccga gcgggccgat gacgatgaac
     2821 cccgcgatct actccaagct gccgtattcg ccgctgcgcg actttgcccc catcaccatg
     2881 atcggctcgt ttccgctcat cctcgtggtg agcccatcgc tgccggtcaa aacggtgaag
     2941 gaactggtcg cgtacgccaa agccaactcg gacaaggtca gttacggcgc gagcgcggcc
     3001 gcgttccagc tcacgaccga gctgttcaaa cagaggaccg acacccgctt tattcacgtt
     3061 ccctacaagg gcgtcaacga atcggtcaat gccgtcatgg cgggccaggt gacgatgacg
     3121 atcggcgatc cggcggtgat cgtgggactg atgaaggccg gcagagtacg ggcgcttgcc
     3181 gtgacgtcag caacgcgcca tccggtgtgg cccgagcttc cgacactttc cgaggcggga
     3241 gtccccgaca tcgaagtcgt gctctggacc ggatttcacg ctccggtccg cacgcccgcc
     3301 gccgcgatta ggaaatttcg cgaggaggtc gttcgagtga ttcggttacc cgaaatccgg
     3361 gagcggctgg ccgccatggc gatcgagccc tcggggaacg cgccggagga atacaagcgc
     3421 atcatcgcct cggacatcaa gaagtggacc gccgtagcca aggcggcgaa catcaaggcc
     3481 gactgaaatc atttcccgcc ctcggcgcga aatgatttat atgaaaagaa tgtatcagga
     3541 ttcccgcacg gtgcgcttgg ggcatactgt acgcggactg cgattgcggt agcgattgat
     3601 cgcgcaaagg cgggaatcct catggcaaga atcatcggcg gcatcggcac ctcccacgtg
     3661 cccacgatcg cgatggcgta cgaccggggc aagcgcaacg acccggactg ggcgccgctg
     3721 ttcaagggct atgagccggt ggcggcctgg ctcgcggcac gccggcccga cgtgctggta
     3781 ttcttctaca acgaccacgc gaccaccttc ttcttcgacc actacccgac cttcgcgctc
     3841 ggggtgagcg ccgaataccc gatcgcggac gaaggcctcg ggccgcggcc ggtgccaccg
     3901 ttgaagggcc acgcgccgct cgcgcgccac ctggccgagt cgctggtcaa cgaggaattc
     3961 gacatctcgg tgttccagga cctgccgatc gaccacggct gccattcgcc gctcacgatg
     4021 ttctggccgc ccgcgcccga ctggcccggg agcatcgtgc cgatcgaggt caacgtgctc
     4081 cagcacccgc tgcccacgcc gatgcgctgc tacaagctcg ggcaggcggt gcggcgcgcg
     4141 gtcgagtcct atcccgggga cctgaaagtg gtcgtggtgg gcaccggcgg gctctcgcac
     4201 cagctgaacg gcgagcgcgc gggcttcaac aacccggaat gggacgcgcg cttcctcgat
     4261 ctcatcgagc gcgatccggg cgcgctcgcg gcgatgaggc acgccgacta tatccggctc
     4321 ggcggcaccg agggcgcgga ggagatcatg tggctcgcga tgcgcggagc gctctccgac
     4381 cgggcgagga aaatccatca gaactgctac ctgccgatga cgaccgcgat ggcggttgcg
     4441 ctgttcgaag agccgtaatg gacggaattc acaggatcga gccaaaattg ctcagggcgt
     4501 gctcacgcag aagccgcggg tgtactcgga tcgtgacggc gcgatccgcg gttacgatcc
     4561 cgtggcctac agccatggtg tccgggaacg ccggaagaag gacgtccccg gccatatccg
     4621 caagggcgac gcgaactggc ctgcggtgct gcagcgttag tgaaatgggg acttttcgtg
     4681 ccgatccgtg tatgatgccg gctcgggatc cgagcaccgc gcgcggtcag cgccggatct
     4741 aacctgcacc ccaccacctt ctgcgacgga catttgcatg agatgccatc actgcggcaa
     4801 gctgaccgtc gtgcgcaccc tcggcaaggg cgaggagttc ctgcaccgct gccggcattg
     4861 caacggcttt cacaagttca ccagcgcggg gcgccacggc tccggcgcca tcaactatcg
     4921 cgtcaccaaa gtcctgcctg cgcctgccag caaatagcgc cgtggcgggc ccgaagcagg
     4981 gcttgcctgg cgctgtcccg cacccactcg actaccgcgc gcggcgttcc atcatttttt
     5041 tcaaccagga ggttgcttgt gccaatcgga acagtgaaat ggttcaaccc ggcgaagggc
     5101 tacggcttca tccagccgga tgacggctcg aaggacgtgt tcgtgcacat ctcggccgtg
     5161 gagcgctccg gcctcggcac gctcaaggag gggcagaagg tccagttcga ccttgcccgc
     5221 ggcaaggacg gcaagacctc ggccgacaat ctgaagtcgg cctgatcggg ccgcgagccg
     5281 tgccgggccg gattcccgga acccggttgc ggcccaattt tgtcgcgggc tcggctgcgc
     5341 ggccgctgcg gcacgcgcag cgatggagga tggcaaagac gcatgaataa gtgacgggta
     5401 acgggtgttt atcacccatc acccatcacg cttcgttgga gccgggtcgc cggaagaagg
     5461 aggtcatcat gacgaagaga gcggatattc ccgtcataac ccactgcgcc gggccccacg
     5521 atgggcatat ctcctggcag cgcatgttgt cctcggcggt cggcgccgcg gcgctcatca
     5581 cggcaacaac ggcgtccgcg gcggggccgt cagctgaggg cgatgtctac gtctacaggc
     5641 tcgtcaacgg ctacaacaag gaagtcgtcg ggcagctccg ctaccaggtc agcaaggtcg
     5701 agcggggcaa cgtcaccttt tcggtcacgc cggacaaccc gtccgcgggg gtggagcgga
     5761 ccgaaatctt cacgaaggag ggcaactggc tgcggcattt gctcgaaaac cacggccaga
     5821 aggtggagta cgagttcgcc gcggcttatc ccgcctacgt gtttccgctc gagccgggga
     5881 aatcctggtc gctgcgcgtc agcgccacgg ttcccaccac cgggcagcgc agaagcgtgc
     5941 gcgtggacgg cagggtgatc gggaacgagc gcatccgcgt gccggcggga gagttcgaca
     6001 ccgtcaagat ccggcgtctg gtgtacccgg gagacgcgga ttactatctt ccggaaacgc
     6061 atatcatcga gttcgactgg tacgcgccgg cgctgggtcg cacggtgcgc accgagagaa
     6121 agtccgacta cttggacctc acccgttgcc atgaggaatc cagctgcgtt taccacggcg
     6181 actgggacgt gttcgaactg gtcgacatac gcgccctgaa gccgtgattc gtgattcgtg
     6241 actcgtgact cgtgagtcgt tcccgttata tttccctcgc gtcctgatcg gccggcatgg
     6301 ccggggctgt tcgccgtatt caagccagcg cttcctggtg ctggcgctgt gcctgctgat
     6361 cggcggctgt acggtatcgg tcggggtgtc caacagtccc gattggtgcg cgaatccgcg
     6421 aaacgcagta tcgtgcccgc cgtgaggaca gagtgatgaa ggcggaggga tgatgaccaa
     6481 ggcggaaggc ggaaggcaga aggcagaagg aagacggcaa gaggcaagcg gagcaggatg
     6541 gtttcacttc atccttcatc cttcttcctt catccttcag gtcaccgcct tcatcctttg
     6601 ctgtgcgttt acggtgaatg ctgcaaacgc acagcagtat ccctcacggc cgatccgcat
     6661 cgtcgtgccg tttccgccgg gcggggcttc cgacgtgacg gcacgcatgc tcgccgacaa
     6721 attcgcgtcg tcctggggcg cgaacgtggt ggtggacaac cgcaccggcg cgtccggcat
     6781 catcggcacc gacatcgtgg cgaaggccgc ccccgacggc tacacgctgg
//
LOCUS       DBNGWZ010000557         2533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_706944, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000557 DBNGWZ010000000
VERSION     DBNGWZ010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_706944"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16CX_17340"
     CDS             complement(<1..245)
                     /locus_tag="AC16CX_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein"
                     /protein_id="HIR3014308.1"
                     /translation="MKRIVVAVIGLTAVAVMSGCAPGVGGGSYSREQARREQNVRMGV
                     VESVREVQIEGTRSGIGPAAGAVVGGIAGSGVGQGRGA"
     gene            complement(259..1194)
                     /locus_tag="AC16CX_17345"
     CDS             complement(259..1194)
                     /locus_tag="AC16CX_17345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3014309.1"
                     /translation="MRTLICGSIAYDTIMVFRDRFKNHILPDRLHILNVAFLVPDMRR
                     EFGGCAGNIAYTLKLLGGEPLIMATVGDDYMPYDHRLKKLGLPQQHIRHIPNTFTAQA
                     FITTDLDDNQITAFHPGAMNYSHENHVHEANGVGLGIIAPDGREGMLQHAREFHAAGV
                     PFIFDPGQGLPMFSREELTGLMNMASYVTVNDYEGEMLQERTGKKIQELAKLVKAFVV
                     TLGARGSLIYVDGQQIEIPCVKAEEVVDPTGCGDAYRAGLLHGIAAGLDWQLTGRLAS
                     LVGALKIAKRGGQNHHFTCDEIAQRFKEQFGTRIW"
     gene            1350..1775
                     /gene="mscL"
                     /locus_tag="AC16CX_17350"
     CDS             1350..1775
                     /gene="mscL"
                     /locus_tag="AC16CX_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3014310.1"
                     /translation="MLKEFREFAVRGNVVDMAVGIIIGAAFGKIVDSLVKDVIMPPIG
                     LLLGKVDFSNLFVVLKQGAQAGPYLTVEAAQKAGAVTFNYGVFVNTVISFMIIAFAVF
                     LLIRVINRLKREETQKPAGPPPTPEEILLLREIRDALKK"
     gene            complement(1890..2297)
                     /locus_tag="AC16CX_17355"
     CDS             complement(1890..2297)
                     /locus_tag="AC16CX_17355"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3014311.1"
                     /translation="MKRDEEAAAGTALPQSPHKGKTGLRRIWNAFCYSLEGLAAAFRH
                     EDAFRQEVLIAVILMPAALLTPASGTGKALMIASVLLVLIVELLNSALEAAVDRISLE
                     SHPLTKRAKDIGSAAVFLSLVNVPIVWFLVLLG"
     gene            complement(2294..>2533)
                     /locus_tag="AC16CX_17360"
     CDS             complement(2294..>2533)
                     /locus_tag="AC16CX_17360"
                     /inference="COORDINATES: protein motif:HMM:NF044806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3014312.1"
                     /translation="GFHAPTAYFPLLVPECLLIEPTETESKQTLDAFVAAMKEILHEA
                     RTQPELVKGAPHTTPVRRLDEVRAARELDLAWKPR"
BASE COUNT          437 a          825 c          791 g          480 t
ORIGIN      
        1 gcgccccggc cctggcccac tccgctgccg gcgatgccgc cgacgaccgc gccggccgca
       61 ggcccgatgc cgctgcgcgt gccttcgatc tggacctcac gcacgctttc caccacgccc
      121 atgcgcacgt tctgctcgcg gcgcgcctgc tcccgcgaat acgaaccccc gcccacgccc
      181 ggtgcgcagc cgctcatgac cgccaccgcc gtcaaaccga taaccgccac aacgatccgt
      241 ttcatgtagt ctccttcgct accagatgcg agtgccgaat tgctccttga aacgctgcgc
      301 gatctcgtcg cacgtgaaat ggtggttctg cccgccgcgc ttggcgatct tgagcgcgcc
      361 caccagcgag gcgagcctgc ccgtcagctg ccagtcgagc cccgcggcga tgccatgcaa
      421 cagacccgcc cggtaagcgt cgccgcagcc ggtcgggtca accacttcct cggccttaac
      481 gcacggtatt tcgatctgtt ggccatcaac gtaaatgagc gagccccgcg cgcccaatgt
      541 gaccacgaaa gccttcacaa gtttcgccag ttcctggatc ttcttgccgg tgcgctcctg
      601 cagcatctct ccttcgtagt cattcaccgt cacgtagctc gccatgttca tgagcccggt
      661 cagctcctcg cggctgaaca tcggcagtcc ctgtccggga tcgaaaatga acggcacgcc
      721 cgcggcgtgg aactctcgcg cgtgctgcag catgccttcc cgcccgtcgg gcgcgatgat
      781 accgagcccg accccgtttg cttcgtggac gtgattctcg tgcgagtagt tcatggcacc
      841 gggatgaaag gcggtaatct ggttgtcgtc gaggtcggtg gtgatgaagg cctgggcggt
      901 gaatgtattc gggatgtgcc ggatgtgttg ttgcggcagc ccgagcttct tgagccggtg
      961 gtcgtacggc atgtagtcgt cgccgacggt ggccatgatg agcggctccc cgcccagcag
     1021 tttgagcgta taggcaatgt tccctgcgca gccgccgaat tcgcgccgca tgtccggcac
     1081 caggaacgcg acgttcagga tgtgcagccg gtcggggagg atgtggttct tgaagcggtc
     1141 gcggaacacc atgatggtgt cgtaggcgat cgagccgcag atcagcgtgc gcatgttccg
     1201 ctctgaagag gggcgaaatt atagcatcgc ggtccgatgg tccggcccgc gccggctgtg
     1261 acaacaatca cccgcggcgc tcataattca tcgcgacccc gcggaagccg tggaggaatc
     1321 cgcatcatcc attcaaggag gtttcaatca tgttgaagga gttcagggag ttcgcagtgc
     1381 gcggcaacgt ggtggacatg gcggtcggca tcatcatcgg cgccgccttc ggcaagatcg
     1441 tcgattcgct ggtgaaggac gtcatcatgc cgccgatcgg cttgctgctc ggaaaagtgg
     1501 acttctccaa cctgttcgtg gtgctgaaac agggagcaca ggccggcccg tatctgaccg
     1561 tggaagctgc gcagaaggcg ggcgcggtca ccttcaacta cggggtgttc gtcaacaccg
     1621 tgatctcgtt catgatcatc gctttcgccg tgttcctgct gatccgcgtc atcaaccggc
     1681 tgaagcgcga ggaaacgcag aaacccgccg ggccgccgcc aacgcccgaa gaaatcctcc
     1741 tgctgcggga gattcgcgac gccctcaaga agtgaggagt gaggggttag gggtgaggag
     1801 agtcagcgta aaaccacgcc cgttgaacat tgcagggttc tttcccctca cgcctcacac
     1861 cttacccctc actttcctcg tgctgctggc tagcccagca gcacgaggaa ccagacgatc
     1921 gggacgttga cgagcgacag gaacacggca gcgctgccga tgtccttcgc gcgcttggtg
     1981 agcgggtggc tctccagcga gatgcggtcg accgcggcct cgagcgccga attgagcagc
     2041 tcgacgatca gcaccaggag cacgctcgct atcatcagcg ccttgcccgt gccgcttgcc
     2101 ggcgtgagca gcgcggcggg catgagaatg accgcgatca ggacctcctg gcggaacgcg
     2161 tcctcgtgtc ggaaggcggc ggcaagccct tcaagcgagt agcagaacgc attccagatc
     2221 cgcctgaggc cggtcttccc cttgtgcggg ctctgcggca acgccgttcc cgcggccgct
     2281 tcttcgtccc gcttcatcgc ggtttccacg cgagatcgag ctcgcgcgcg gccctgactt
     2341 catcgagccg gcgcaccggc gtcgtgtgcg gcgcgccttt cacgagttcc ggttgggtgc
     2401 gcgcctcgtg caggatctcc ttcatcgcgg cgacgaaagc atcgagcgtc tgtttcgact
     2461 cggtctcggt cggctcgatc agcaggcatt cgggcacgag cagcgggaag tacgcggtcg
     2521 gggcatggaa gcc
//
LOCUS       DBNGWZ010000558         3665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2345976, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000558 DBNGWZ010000000
VERSION     DBNGWZ010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2345976"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="AC16CX_17365"
     CDS             complement(<1..123)
                     /locus_tag="AC16CX_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3014313.1"
                     /translation="MNRIVHLALKVENLEKATEFYEKVFGFREVETRKTRDHISR"
     gene            complement(292..996)
                     /locus_tag="AC16CX_17370"
     CDS             complement(292..996)
                     /locus_tag="AC16CX_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3014314.1"
                     /translation="MAIVRILSAGAAQAMVEKIAVAYTRDTGHKVEADFSAVGAMKAR
                     VVAGEPVDVIVLTGALIDDLIASGHVAPRSRADLGKVGTGVAMRAGTQAPDISSAPAL
                     RGSLLAATKIAFPDPGTATAGKVVMQVFEQLGIAEQVRPKMQFFRNGYAAMQWLAASR
                     GPLEIGVTQITEILANPGVTYAGPLPTDLQTKTVYAAGLAARAGEPDAAREFIGRLTR
                     PSARGVLAQAGYEFDN"
     gene            1156..1479
                     /locus_tag="AC16CX_17375"
     CDS             1156..1479
                     /locus_tag="AC16CX_17375"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR3014315.1"
                     /translation="MKQIHVAKHAPEAHLVKGFLESNGIAAVVRGEFLTSGWGELPVD
                     LCSVWIENDAQYDRANALLVAFLRGDFAREFRDQNWRCPKCGENLEGQFTACWKCGAS
                     KPGRT"
     gene            complement(1499..1918)
                     /locus_tag="AC16CX_17380"
     CDS             complement(1499..1918)
                     /locus_tag="AC16CX_17380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014316.1"
                     /translation="MSARLSVASMTIPWSRSTARWRSLSASPGAIVTSSPISSSDFLR
                     SPALPELPTIDEAGVPGFDVTVWGGVVVPSGVPKTIVARLNTEINNALASTTLKEKFS
                     AISYELVGGTPEQFDAFVRKEIAKWADVIKRSGAKVD"
     gene            complement(1800..2162)
                     /locus_tag="AC16CX_17385"
     CDS             complement(1800..2162)
                     /locus_tag="AC16CX_17385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR3014317.1"
                     /translation="MTMRRGDLVTVAAPGDISKPRPALVIQSDLFDELPSVALCLVTS
                     TLRDAPIFRITVDPSPANGLQRVSQVQVDKIVAVAREHVGAVIGRLDDDTLVKVNRSL
                     AVFVGIAWGNRHFVTDFQ"
     gene            complement(2143..2337)
                     /locus_tag="AC16CX_17390"
     CDS             complement(2143..2337)
                     /locus_tag="AC16CX_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HIR3014318.1"
                     /translation="MKGYRVRRRAAGLRLVQLWVPDTRSPGFAAECRRQSRLIRGDPA
                     ETGALSFIERAAAWDDDAAR"
     gene            complement(2445..>3665)
                     /locus_tag="AC16CX_17395"
     CDS             complement(2445..>3665)
                     /locus_tag="AC16CX_17395"
                     /EC_number="2.8.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0046952 - ketone body catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HIR3014319.1"
                     /translation="DPGATRGQILAENRQVKKLIAAFSVRPGTPTASEEQIAAGEMEV
                     ELVPQGILVERCRAGGAGIPAFYSPTSVGTELVKGKEIREFDGKPHVLEHAIHVDYAF
                     LRGYRADRLGNVQFRGGSQNFNPSFAKAARVAIVEVDEIVEPGEIPPELVNLPGIFIS
                     RVVKTTQKFDLWHRPERRPSDQPRLYNGKPALTRAGIAKNAARLVRDGSYVNLGVGIP
                     TMVSNYLVGRDVILHAENGVLGYGRMVTEENEIDPDIYNAAGQYVALKPGASFFDSVT
                     SFEMARGGRVDTVILGAYEVDQTASVANWSTADAKRGGIGGAMDLLSGKGDLVIVMEH
                     TDSKGRPKLRKKCTYPLTGKGCVTHVVTDLALLRWDGGRFVLDEVAPGFTPQKVIALT
                     EMEIYVGPDVRTMS"
BASE COUNT          684 a         1175 c         1096 g          710 t
ORIGIN      
        1 gcgcgagatg tgatcgcgtg tcttgcgcgt ctccacttcc cggaaaccga acaccttctc
       61 gtagaattcg gtggcctttt cgaggttttc gaccttcaac gcgagatgca cgattctgtt
      121 catcaaaagc tccggtaagc ggtaagcggt aagcggtaag cggtaagcgg taagcggtaa
      181 gcggtaagcg gtaagcggta agcggtaagc ggtaagcggt aagcggtaaa tttagatatg
      241 cctgctggtc gaagttcacc gctcaccgtt cactgctcac cgttcacggt ctcagttgtc
      301 aaattcgtag cctgcttgtg cgagcacgcc ccgtgccgag ggcctggtga gccgcccgat
      361 aaattcccgt gccgcatccg gttccccggc ccgcgccgcc agccccgccg cataaaccgt
      421 tttcgtctgc agatccgtgg gcagcgggcc ggcgtatgtt acaccggggt tggccaggat
      481 ttcagtgatc tgagtgacgc ctatttccag cgggccccgg cttgccgcaa gccactgcat
      541 cgccgcgtaa ccattacgga aaaactgcat cttcggcctc acctgctccg cgatgccgag
      601 ctgctcaaac acctgcatca cgacctttcc cgcggtcgcc gtccccgggt cgggaaaggc
      661 gatcttcgtc gcggcaagca ggctgccgcg cagcgccggc gcgcttgaaa tatccggcgc
      721 ctgcgtgccc gcccgcatcg ccacgcccgt accgaccttg ccgaggtcgg cgcgcgagcg
      781 gggtgcaacg tggccggatg cgatgagatc gtcgatcagc gcgccggtga gcacgatgac
      841 atccactggc tcgccggcaa cgacccgcgc tttcatcgcg cccaccgcgc tgaagtcggc
      901 ctcgaccttg tggccggtgt cgcgcgtgta ggccacggcg atcttctcca ccatcgcctg
      961 cgccgccccc gcgcttaata tccggacgat cgccatctct gcattcggcc tgaaaagcgt
     1021 gaatcgtgat tcgtgaaaat tgtttccctt cctccggtcg gaggagaggg tttcaccatt
     1081 cactattcac cattcactgt ttagggatgc tagcacacgt gcgcagcctt acgcggaggc
     1141 gtaaagtaac ccatcatgaa gcagatccac gtcgccaagc acgcgccgga ggcgcatctc
     1201 gtcaagggct tcctcgagtc gaacggaatc gcagccgtgg tgcgcggcga attcctgacg
     1261 agcggctggg gcgagctgcc tgtggacctc tgctcggtgt ggatcgagaa tgacgcgcag
     1321 tacgaccgcg cgaatgcgct gctcgtagcg ttcctcaggg gcgatttcgc gcgcgagttt
     1381 cgcgaccaga actggcgctg cccgaaatgc ggcgagaacc tcgaaggtca gttcaccgca
     1441 tgctggaaat gcggcgcctc gaaacccgga agaacgtgat gagtgatggg tgacgggttt
     1501 agtcaacctt tgcacctgag cgcttgatga catcggccca tttcgcgatt tccttcctta
     1561 cgaacgcgtc gaactgctcc ggagtgccgc ccacgagctc atagctaatt gcggagaatt
     1621 tttccttcag cgttgtcgat gcgagggcat tgttgatctc ggtattgagc ctcgcgacga
     1681 ttgttttcgg aacgccggac ggcaccacca ctccgcccca gaccgtcacg tcgaaaccgg
     1741 gcacacccgc ttcgtcgatc gtcggcagct cggggagagc gggcgagcga aggaaatcgc
     1801 tactggaaat cggtgacgaa gtgacgattg ccccaggcga tgccgacaaa gaccgccagc
     1861 gagcggttga ccttgaccag ggtatcgtca tcgaggcgac cgataaccgc gccgacatgc
     1921 tcgcgcgcaa cagcgacgat cttgtcaacc tggacctgcg aaacccgttg cagaccgttt
     1981 gccggagacg ggtcaacggt gattctgaat atcggcgcgt cgcgcaaagt gctggtgacg
     2041 agacacaagg cgacgctcgg caactcgtcg aacaggtccg actgaatcac caacgcggga
     2101 cgcggtttgc tgatgtcgcc gggtgcggca accgtgacca aatcaccgcg ccgcatcgtc
     2161 atcccaggcg gccgcgcgct cgatgaaact cagagccccg gtttccgccg ggtcgccacg
     2221 tatcagccgc gactgacggc ggcattccgc ggcaaatccc ggtgacctcg tatcgggcac
     2281 ccagagctgc accaggcgca agccggcagc ccgtcgccgg acgcggtagc ccttcatgcg
     2341 ctccttagcc tccatggcat ccccgtaaca tgttactcgc aatgttacag gatagatcac
     2401 aattaatcaa tgagttattc gacgaaaatt gggacgagcg cctctcaaga catggtacga
     2461 acatccggcc cgacgtaaat ttccatttcc gtcagcgcga taactttctg cggcgtgaat
     2521 cccggcgcca cttcatcgag gacgaagcgg ccgccgtccc agcgcagcaa cgccaggtcg
     2581 gtgacgacgt gggtaacgca gcctttcccc gtcaggggat aggtgcactt cttccgcagc
     2641 ttcgggcggc ccttgctgtc ggtgtgctcc atcacgatga cgagatcgcc cttgcccgat
     2701 aggagatcca tcgctccgcc aatgccgccg cgcttcgcgt ccgccgtgct ccagttggcg
     2761 acactagcgg tctggtccac ctcgtacgct ccgagaatca ccgtgtcgac cctgccgccg
     2821 cgcgccatct cgaacgaagt cacgctgtcg aagaacgatg ccccgggctt gagcgcgaca
     2881 tactggccgg cggcgttata gatatccgga tcgatttcgt tttcctccgt caccatgcgg
     2941 ccgtagccca ggacgccgtt ctcggcatgc aggatcacgt cgcggcccac caggtagttc
     3001 gacaccatcg tgggaatgcc gacacctagg ttgacgtagc tcccgtccct gacgagccgg
     3061 gcggcgtttt tcgcgatgcc agcacgcgtc agggcaggct tgccgttgta gagacgcggc
     3121 tggtcggacg gccgccgttc tgggcgatgc cagagatcga atttctgcgt cgtcttgacg
     3181 acacgcgaaa tgaatattcc cggcagattg acaagctccg gcggaatttc gccgggctcc
     3241 acgatctcgt cgacttcgac gatcgcgacg cgggcggcct tggcgaagct cggattgaaa
     3301 ttctggctcc cgccgcggaa ctggacattg ccgaggcggt ccgcgcgata tcccctcaga
     3361 aaagcgtagt ccacatggat cgcatgctcg aggacatggg gcttgccgtc gaattcacgg
     3421 atttccttgc ccttcacgag ctcggtgccg acgctggtcg gcgagtagaa cgccggtatc
     3481 cccgccccgc ccgcgcgaca ccgttccacc aggatgccct gcggaaccag ctcgacctcc
     3541 atctccccgg cggcgatctg ttcctcgctc gcggttggcg tgccgggacg gactgaaaac
     3601 gcggcgatca gctttttcac ctgcctgttc tcggcgagga tctggccgcg ggtggctccc
     3661 ggatc
//
LOCUS       DBNGWZ010000559         2823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1341232, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000559 DBNGWZ010000000
VERSION     DBNGWZ010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1341232"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16CX_17400"
     CDS             complement(<1..218)
                     /locus_tag="AC16CX_17400"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3014320.1"
                     /translation="MKVYQRLASAFKAEGVSAIFGIMGDGNMYWLHELDKLGVKLLEV
                     RHEGSGLGMADGWARATHEPGVATATCGP"
     gene            complement(331..822)
                     /locus_tag="AC16CX_17405"
     CDS             complement(331..822)
                     /locus_tag="AC16CX_17405"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR3014321.1"
                     /translation="MIEISERIEVPSAPRIVWGLLSDPRAVVECVPGAALGDQHEDGS
                     FDASLTVKFGPAKVTFRAKVALELDAAAMSGRVISRGKDNQGGTRFHAAMNFKVEEQK
                     EPPGSAIPIAAQVEISGRLATLVEGGAALVVKRMTGEFSKRLAARCAAGNQAEGEGAR
                     LIS"
     gene            complement(1069..2037)
                     /locus_tag="AC16CX_17410"
     CDS             complement(1069..2037)
                     /locus_tag="AC16CX_17410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014322.1"
                     /translation="MNALRRIAVISLAFALPGVAPAQDKWPSKPIRMIVPFTAGAASD
                     VLGRIVGQRLSEIYGQQIVIDNRPGAGGLIGSELTRQAAPDGYTLAMVGQPHLSNALL
                     REDKPYHPLKDFTAISLVGSTHNVIVLGKGVNAKTIPELIALARAKPGTLNYGSAGVG
                     SSSHLAGALFASKAKIDVVHVPFRIGADSRSALIAGTIHYYVYPLPAIVGMLKAGTLR
                     ALAVASPKRAESLPEVPTSAEAGFGEYRSESWFGLIAPRGLPRQLVTRINADAVGVLK
                     EPATREKIVQQGAQPGFGPPEHFDKMLRDEYAELAALIKQIGMKTQ"
     gene            2145..>2823
                     /locus_tag="AC16CX_17415"
     CDS             2145..>2823
                     /locus_tag="AC16CX_17415"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3014323.1"
                     /translation="MIERQRHPVKAYRNQAFLNSKDARALRILSEYLEPKSRFERHRV
                     DDTIVFMGSARIKPREQAEEALRKAEGGGGDVESARMALRMSGYYEAARELAFRLTQW
                     SKELDPKTRRFVVCTGGGPGIMEAANRGAAEARGMNVGLTISIPLREFDNPFVTRELA
                     FHFHYFFMRKFWFAYLAKAVIVFPGGFGTLDELFELLTLVQTRKMSKPMPIVLFGTEY
                     WRQVVDFD"
BASE COUNT          488 a          936 c          882 g          517 t
ORIGIN      
        1 gggccgcagg tcgcggtggc gaccccgggt tcatgcgtcg cccgcgccca accgtccgcc
       61 atgccgaggc ctgacccctc gtgacggact tcgagcagct tgacgccgag cttatccagc
      121 tcgtgcagcc agtacatgtt gccgtcgccc atgatgccga agatggcgga aacgccttcc
      181 gccttgaacg cgctcgccaa ccgctgatac accttcatct gtgatctccc attgtcagtg
      241 actcgcgact cgtgactcgt gagtggtgtt cgagaaacgg ttaactgtca cgaactttaa
      301 agggccgacc cgcgagtcac gactcgcgag tcacgaaatc aggcgggctc cttccccttc
      361 cgcctgattt ccggcggcgc agcgggcggc gagccgcttt gagaattcgc ctgtcatgcg
      421 cttgaccacc aatgcggcgc ccccctccac cagtgtggcg agccggcccg aaatctcgac
      481 ctgcgccgcg atcgggatcg ccgaacccgg cggctccttc tgctcctcga ccttgaaatt
      541 catcgcggcg tgaaagcgcg tcccgccctg gttgtccttg cctctcgaga taacgcgacc
      601 ggacatcgcc gccgcatcca gctccagcgc gaccttggcg cgaaaggtca ccttggcggg
      661 cccgaacttg acggtcaggc tcgcgtcgaa cgacccgtcc tcatgctgat cgcccaatgc
      721 cgcccccggg acacattcga caacggcgcg cggatccgac agcaggcccc atacgatccg
      781 cggcgccgat ggcacctcga tacgctcgct gatttcgatc acgatgtgga ctgcagagaa
      841 aaacgttccg gggccgcaat agtaacagca agcggcgcca gccgctcttc aagctcgtga
      901 ttcgtgaatg gtgagtggtg agtggtgagt ggtgagtgga cgccgggccc gtggtccgac
      961 ccttggactc gcgactcgat ccggctttcg tcacctcgtt actgcgtcac ttcgtcactg
     1021 gggtaatccc ccatcacccg tcacccatca cccatcaccc atcacgcatc actgagtctt
     1081 catgccgatc tgcttgatca acgcggcgag ctcggcgtat tcgtcgcgca gcatcttgtc
     1141 gaagtgctcg ggcgggccga agccgggttg cgcgccttgt tgcacgatct tctcgcgcgt
     1201 ggccggctct ttcaggacgc ccaccgcgtc tgcattgatc cgcgtgacga gctgccgggg
     1261 cagcccgcgc ggcgcgatga ggccaaacca tgactccgag cggtattcgc cgaagcccgc
     1321 ttccgcgctg gtgggcacct cgggcagcga ctcagcccgc ttcggggagg caaccgccag
     1381 cgccctcaac gtgcccgcct tcagcatgcc cacgatcgcg ggcagcggat aaacatagta
     1441 gtggatcgtg ccggcgatca gcgcgctgcg gctatccgcg ccgatgcgaa acggcacgtg
     1501 cacgacgtcg atcttcgctt tcgaggcgaa tagcgcgcca gcgagatgcg aggagctgcc
     1561 caccccggcc gagccgtaat tgagcgtgcc gggctttgcc cgggcaaggg cgatcagttc
     1621 cggaatggtc ttcgcgttga cgcctttgcc gagcacgatc acgttgtggg tcgagccgac
     1681 cagcgagatc gccgtgaagt ccttcagggg atggtacggc ttgtcttcgc gcaacagcgc
     1741 attcgacagg tgcggctggc cgaccatggc gagcgtgtag ccgtccggcg cggcctggcg
     1801 cgtgagctca ctgccgatca gcccgccggc acccggccgg ttatcgatga cgatctgctg
     1861 gccgtagatc tcgctcaagc gctgcccgac gatgcgcccg agcacgtcgc ttgcagcgcc
     1921 cgcggtgaag ggcacgatca tgcgtatcgg tttcgacggc catttatcct gtgccggcgc
     1981 cacgcccggc agcgcgaaag cgagcgaaat caccgcgatc ctgcgcaatg cattcatgct
     2041 ggtctccccc ggtccgtgtt gttatattac ttcagcaacg gggtcgggtc ttgtattgaa
     2101 gcacgtcgtt gcaagacctg tcccccatcg atataggacc ctcaatgatc gaacgccagc
     2161 gtcatcctgt gaaggcctat cgtaaccagg cgtttctgaa cagcaaggac gcccgggcgc
     2221 tccgcatcct gtcggaatat ctcgagccga aaagccgctt cgagcgccac cgcgtcgacg
     2281 acaccatcgt gttcatgggc tcggcgcgga tcaagccgcg cgagcaggcc gaggaggcgc
     2341 tgcgcaaggc cgagggaggc ggcggcgacg tagagagcgc gcgcatggcg ctcaggatgt
     2401 cgggctacta cgaagccgcg cgcgagctcg cctttcggct cacgcagtgg tccaaggagc
     2461 tcgacccgaa gacacggcgc ttcgtggtgt gcacgggagg cggcccggga atcatggaag
     2521 cagccaaccg gggcgcggcc gaggcccgcg gcatgaacgt gggcctgacc atctcgatcc
     2581 cgctcaggga gttcgacaac ccgttcgtca cgcgcgagct ggcatttcac ttccactact
     2641 tcttcatgcg caagttctgg ttcgcctatc tcgcgaaggc ggtgatcgtg tttccgggcg
     2701 ggttcggcac gctcgacgag ctgttcgagc tgctgacgct ggtgcagacg cgcaagatga
     2761 gcaagccgat gccgatcgtc ctgttcggca ccgagtactg gcggcaggtg gtggatttcg
     2821 acg
//
LOCUS       DBNGWZ010000560         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2724026, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000560 DBNGWZ010000000
VERSION     DBNGWZ010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2724026"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="AC16CX_17420"
     CDS             <1..813
                     /locus_tag="AC16CX_17420"
                     /inference="COORDINATES: protein motif:HMM:NF018032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HIR3014324.1"
                     /translation="ASLDRLADAFAEIVIAMLRQEAPAGLAKDLLVTEPLASDPGPHL
                     ITFTGGVSEYIFGRESATFGDIGKPLAERIVEAFSSGRVPVKMMDTGQGIRATVIGAS
                     QFTVQVSGNTIHLSDGIRLPLRNVPVAFPHLAPERDFDAAAVAAAIRAAVERIDAEAG
                     RPVALGIRWHGDPHYHRLRGLAEGIVQTLGPAGPEPLVLMVDGDFGNLLGHILKDELG
                     VARGVVSIDGIQLRELDYVDIGEVIKPSNVVPVVIKSLLFAPASSLHKPVVS"
     gene            837..>1885
                     /locus_tag="AC16CX_17425"
     CDS             837..>1885
                     /locus_tag="AC16CX_17425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014325.1"
                     /translation="MDESKSPRVGVDSYLEWLKGEGIPAIEDFGVDLFEVDTRPWARL
                     GVDGAAVHLKGRGDFVSMFVLEVAPGASTSPQRHLYEEILYVLAGHGSTTIEAEDGRK
                     HAFEWGPRSLFAIPLNTKYRHFNGSGSETARLVTTTNLPAVLNMFHNEAFVFDNPFRF
                     GERLGQSRYFGGEGDFIPVVPGQHMWESNFVPDLAAIELRSWDQRGAGSKNIMFILAD
                     GTMHAHISEMQVGTYKKAHRHAADFHVMCVTGKGYSLLWYEDDAEFRRVDWKHGTVFA
                     PPDQMFHQHFNTSPTPARYLATAFGSLRYPFTESKRRSLCGGGAGGVKQGGVATSVKE
                     GGDQIEFEDQSPRIHA"
BASE COUNT          326 a          614 c          632 g          313 t
ORIGIN      
        1 gcgagcctgg atcgccttgc cgacgcattc gccgaaatcg tcatcgcgat gctgcggcaa
       61 gaggcacccg ccgggctggc gaaggacctg cttgtcaccg agcccctcgc ctcggaccca
      121 ggaccgcacc tgatcacgtt cacgggcggg gtttccgagt acattttcgg ccgcgagagc
      181 gcgactttcg gcgacatcgg gaagccgctc gccgaacgga tcgttgaagc gttttcgagc
      241 ggccgcgtcc ccgtgaagat gatggatacc gggcagggca tccgcgccac ggtcatcggc
      301 gcctcgcagt tcacggtgca ggtgagcgga aacaccatcc acttgtcgga tggcatccga
      361 ctgccgctgc gcaacgtgcc ggtcgctttt ccacacctcg cgcccgagcg cgacttcgac
      421 gccgcagcgg ttgccgcggc gatccgcgcg gcggtcgagc gcatcgacgc cgaggcgggc
      481 cggccggtgg ctctgggaat ccgctggcac ggcgacccgc actatcaccg cctgcgcggg
      541 ctcgccgaag gcatcgtgca gacgctgggt ccggccggcc cggagccgct cgtcctgatg
      601 gtcgatggcg atttcggcaa tctgctggga cacatcctga aggacgagct cggcgttgcg
      661 cgcggcgtcg tttccatcga cgggatccag ctgcgtgaac tcgactacgt ggacatcggc
      721 gaggtaatca agccgtccaa cgtggtcccg gtcgtgatca agtcgctgct ttttgcgccg
      781 gcgagctcat tgcataagcc ggtcgtttcg tagcaccgca accgaggaaa ccgcacatgg
      841 acgaaagcaa atcgccgcgg gtcggcgtcg actcctatct cgagtggctg aaaggggaag
      901 ggatccccgc gatcgaggac ttcggcgtcg atcttttcga ggtggacacc cggccctggg
      961 cgcgcctcgg ggtcgacggt gcggccgtgc acctgaaagg gcgcggggac ttcgtgtcga
     1021 tgttcgtgct ggaagttgcg cccggcgcct cgacctcgcc gcagcgccac ctctacgagg
     1081 agattctgta cgtgctcgcg ggccacggca gcaccacgat cgaagccgag gacggccgca
     1141 agcacgcctt cgaatggggg ccgcggagcc tgttcgcgat tccgctcaat acgaaatacc
     1201 gccatttcaa cggcagcggg tcggaaacgg cgcggctcgt gacgaccacc aacctgccgg
     1261 ccgtgctgaa catgttccac aacgaggcgt tcgtgttcga caatcccttc cggttcggcg
     1321 agcgcctggg ccagtcgagg tacttcggcg gcgaagggga tttcattccg gtggtgcccg
     1381 ggcagcacat gtgggagtcc aatttcgtgc cggacctcgc ggcgatcgag ctcaggagct
     1441 gggaccagcg cggggccggg agcaagaaca tcatgttcat cctcgccgac gggaccatgc
     1501 acgcgcacat ctccgagatg caggtcggca cctacaagaa ggcgcatcgc cacgccgccg
     1561 attttcacgt gatgtgcgtg accggcaaag gctactcgtt gctatggtac gaggatgatg
     1621 cggagtttcg ccgcgtcgat tggaagcacg gcacggtgtt cgcgccgccc gaccagatgt
     1681 tccaccagca tttcaacacc agcccgaccc cggcgcgcta cctcgccacg gcgttcggca
     1741 gcctgcgtta cccgttcacc gaaagcaagc gcaggtcgct ctgcggcggc ggggccggcg
     1801 gcgtcaagca gggcggcgtg gcgaccagcg tgaaggaggg cggcgaccag atcgagttcg
     1861 aggaccagtc gccgcgcatc cacgc
//
LOCUS       DBNGWZ010000561        13232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_707997, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000561 DBNGWZ010000000
VERSION     DBNGWZ010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_707997"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16CX_17430"
     CDS             <1..430
                     /locus_tag="AC16CX_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014326.1"
                     /translation="KACPGRVMGVNFAGTNDQGVKIKTDMAMRMKQRFEKHLDRIPAD
                     PEIRQALLAIKRETTGTAIRFDAPRIEVDSPVAGGEKRKRFGHADEFWAKAMASLAAA
                     TGARVSTEFTAVRMSQAAQLIRAGYGPGGGQTATLAARGW"
     gene            432..1712
                     /locus_tag="AC16CX_17435"
     CDS             432..1712
                     /locus_tag="AC16CX_17435"
                     /inference="COORDINATES: protein motif:HMM:NF017850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein family protein"
                     /protein_id="HIR3014327.1"
                     /translation="MGTAPIPMRPEAKELVTLEVLRWTGLGAQAFSGTMSPSTIFQMM
                     VQGSPGVMPYYREVEEKDTAVSSALAMRRLLVLARDTAMQPADPQNGQARELSDAAAA
                     FLDSIPRFGFALWELLDAPAYGYSVVETLWQTDGSAVGVERLVGRPQELFRFGRIADP
                     QVGDLVLANFPGGEGQPVPPQKFLVTSYHPRHGDRRGMPLLRRLYWPSWFKRNVLRLH
                     LKFLEKGPGTVAVKYPSGAGDDEKKKALEAAEAIADEIAVAVPETFALIPEALQTTRT
                     RQAEDFRALFDYFDAEMTRIVLGQTLTTRGAEQQRGTQALGQVHENTMWQVVRGDAAD
                     LEAAINEQLLGPWLLWTFGPGALERAVRPWWTIEKDPPRDAAAAADLLVKAHQLVELP
                     TSEVYEMVQVRAPEEGENVVPRSAVPASLFQPGL"
     gene            1745..2662
                     /locus_tag="AC16CX_17440"
     CDS             1745..2662
                     /locus_tag="AC16CX_17440"
                     /inference="COORDINATES: protein motif:HMM:NF016148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HIR3014328.1"
                     /translation="MRPPKIRVTRTASREAVETQLDLEKMLAAAVMEQARYFHAIAGA
                     LLETGRMAFAAGARAIAESAGTGAETLAHIRRLAEILTATRMLGRVRVRKEVGLPLAS
                     DLQFAEVLHGIGVDQAVEYLRSLPVATREEWLRLLEANQGPAFTAAGVENKAALEALR
                     DLVGQALDRGWTAAEFEQGAMELLAKFQEEAGALRTLWNTVTATAMARGREEMLDDPE
                     VKKIVAYRLYDAIIDSRTRPNHAALDGGIAPADWEGWPEYAPPNGFNCRCTLVGITAV
                     RARRMLESNEGFDLTVKIPVGAGPDPGFK"
     gene            2673..4055
                     /locus_tag="AC16CX_17445"
     CDS             2673..4055
                     /locus_tag="AC16CX_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014329.1"
                     /translation="MATVTKKEADGEHPAGHYLVVEDPQQASTWHLRVKTPDGKPDHG
                     LMGAAWAALHEGYRGNRYEGPGKEEAIRKLKALYKDEGMDMPGMNMGDFSNQWIEIFR
                     AGDYGAKGNWTAEDVKRVTANFEPQTWQPPLVLGHPKDDAPAMGLVRELREEAGRLLM
                     RAEKVHPALEALVQEGRYPNRSVAFYLDPEGKGPVLRHVGFLGAQPPEIKGLEPVKFS
                     DGEFVAIEFNEEETVDPKELSKTVAAEIRNFFSSLFGEKKPDATFTEEQVQARIAAAM
                     KPLEQGIADLARKFDESVKQADQRTAASTEAAKKAEVAGFIEKQKAANRWVPAFDAAG
                     LPAVLEQLALKGGTVKFGEAGKEKEAPAFELLTGFLTALPAIVPTKELVRSGKRIGKV
                     VPFTEPTNPSTVVDAESMALAEAAQQLLPEVLKENPKLARHEAYSEAVRRAREAGASK
                     AAGATAAGQA"
     gene            4094..4486
                     /locus_tag="AC16CX_17450"
     CDS             4094..4486
                     /locus_tag="AC16CX_17450"
                     /inference="COORDINATES: protein motif:HMM:NF021473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HIR3014330.1"
                     /translation="MAYGTVGPQVIRSYKADAAIAVGLAVIGGAAVDSVALPGGANVR
                     ALGVTAAAVDAAGKPVSVVEFGEVKAVADAAIARGDYVMVNAATGKLAPIGATAGTNY
                     HVVGIALEAAAAQNDTFALFVCPSRAQG"
     gene            4526..5473
                     /locus_tag="AC16CX_17455"
     CDS             4526..5473
                     /locus_tag="AC16CX_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015802.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HIR3014331.1"
                     /translation="MGAFVSPILAKIDVPLSIFAREYRNNELIADLVAPRVGVQNQSD
                     KYWLFGREGQQIGGNVLRGPGSAAERILQTISTDSYSCQDHALARLITDEERGNFQAG
                     NVEEWATRTVVNQLLLDRENRAAALFTTAANYPTGNKVTLSGTSQWNEATQVSTPVKD
                     VMTAHLAVAKIGQKANTLILGPDVWAALKVNKQITDRVSPSKLGVVSTEDLAAIFEVD
                     QVLIPKGVQVSAAGVASFVWGKHAVLAFIQPGASMADVTFAKSFFWTGAPGTTGGVST
                     EIGRLTPPSAKADELAVHMYYDLKQTSNVSAYLIENAVA"
     gene            5555..5704
                     /locus_tag="AC16CX_17460"
     CDS             5555..5704
                     /locus_tag="AC16CX_17460"
                     /inference="COORDINATES: protein motif:HMM:NF048662.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7210 family protein"
                     /protein_id="HIR3014332.1"
                     /translation="MPKYLVKSPLRHDGQDYEPGETVDLTKKQAEAMPATVEPVEKKA
                     EPEKK"
     gene            5711..6139
                     /locus_tag="AC16CX_17465"
     CDS             5711..6139
                     /locus_tag="AC16CX_17465"
                     /inference="COORDINATES: protein motif:HMM:NF018704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HIR3014333.1"
                     /translation="MAYAAKADLQQRLAPEKLTQLTDFSGTGEADDARIDEALGAASA
                     VIDSYAGGRYALPLAASDQVRDLALALAIHKLYEFRQREVPETVKAGKEAAMALLKDV
                     AAGKATLDQPAKKQTVSSEAVKKDHTTEPDVFDDDRLDKF"
     gene            6139..6687
                     /locus_tag="AC16CX_17470"
     CDS             6139..6687
                     /locus_tag="AC16CX_17470"
                     /inference="COORDINATES: protein motif:HMM:NF016927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HIR3014334.1"
                     /translation="MAAVTIRLDLKRVQRMLGLTQQALERGLVLAVAEWLRLAIRRSF
                     EQEQSPEGRQWPALSPVYAQTKKRGRRILERTGALFNQAVNSVTIAGNAVLAGAWLPY
                     AAIHQYGGAIRIPEIVPRKGAALRWLGPSGEAIFARRVKAHTVTIPARPYLPSPEWAE
                     LESKRVIEETIQDAIDQGEGQK"
     gene            6684..7157
                     /locus_tag="AC16CX_17475"
     CDS             6684..7157
                     /locus_tag="AC16CX_17475"
                     /inference="COORDINATES: protein motif:HMM:NF020447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp37"
                     /protein_id="HIR3014335.1"
                     /translation="MSNQTLIAEAEDALIALLKAQLGGATGVAAVTSQDFDEAGDIIA
                     VPPAIRVLFRRETLESGRDATALTYVSLENFAALCGAENLRSIAEERKGAYELVSQVC
                     DILAGARLTLPSKTAGQRPTCILRGVELVQFGAQGTWYAVSFDVESISQMSGVAA"
     gene            7169..7420
                     /locus_tag="AC16CX_17480"
     CDS             7169..7420
                     /locus_tag="AC16CX_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014336.1"
                     /translation="MAREDFVEIRLTKKATEAAGVGTVRIVNALVDVEVRAGETHRVS
                     RAEWTEIFSLARLHGEPVFEIASMPEPVKPAIRQKAERS"
     gene            7424..8419
                     /locus_tag="AC16CX_17485"
     CDS             7424..8419
                     /locus_tag="AC16CX_17485"
                     /inference="COORDINATES: protein motif:HMM:NF039819.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HIR3014337.1"
                     /translation="MATFAAQQIGAREIVISPNKQNAYGTVLGDVNLVHNLRFDGGAF
                     ADLAKEYMTDMDLAKGHPWATFKNPIRQNMRLSLGGLQVYDFLAGWLAAFLFGKVQTS
                     GVGPYTHLFTADLSTTIAPVTTAWIHDTADVKSKVPDLAINSLRFNGAAVGPVTADAE
                     LVGSGRFTDIAMAAPALAVPTYLLNSDADILLGPQGAPVSIKERVRSWSVNLTANLED
                     YRHPGSGLYAGFTRRGDLRITVQLGIAAKDVDDIRTLGVLDTKRELQINVNSGAAAQL
                     KFKFPAVILRVTGGAERTFVLWTIEADDTGVLKDGVNEPIEVTAINNQATYLVGA"
     gene            8588..9376
                     /locus_tag="AC16CX_17490"
     CDS             8588..9376
                     /locus_tag="AC16CX_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014338.1"
                     /translation="MEEAPATAVGIPNETRNDALLPLDLAERAVTWSASGRVRTHVFR
                     RLTAADWEDYIAHIRFEVETGAPPRIDVTVAGLRLYERAVLLADGYPVRGDAKLESLP
                     RWKDLLPIGERLQAVRLLMQVDRHEGDDAAMIEPDIQIAALDAVWGTAGPGRTQTYRG
                     LLHRFTPPTAEHLARYSREISRAKIIGGSRTGLTVYQPREKILVKLYDELIQSVEGYS
                     VGGEPLADREAIVREMDPFHKAAAASIIFDLLGRRDEEEPQDRP"
     gene            9373..9771
                     /locus_tag="AC16CX_17495"
     CDS             9373..9771
                     /locus_tag="AC16CX_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014339.1"
                     /translation="MIEVWRDAEGVRQAMAEMMENGLRARMRRSLAQRMKGAANADRA
                     LAGLDDQRTLADGYYAWAGHLLLLDRVVRQGVLWTALRVEELRGLADLGQVEAEFERG
                     HPRCPRCGEMQTDAAEARCWNCGKEFGAPR"
     gene            9775..12141
                     /locus_tag="AC16CX_17500"
     CDS             9775..12141
                     /locus_tag="AC16CX_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014340.1"
                     /translation="MATTAEIQIVVDQEGAVHAFQQIGNEVKKIEGPTRQLGGTSNAV
                     FTQMRKQQEQARDAAQLLSRVIGAEVPRALEKVLARSQTIGPALSKAFNVAVVAAFAK
                     AAFDAITQAVEYLEIALRSAKEQARLTAAAGSQAERDLVKQTEQAIRETRTRTRLLTA
                     SDLGKISVQLAADLQKIEEQRMTAEALGETRLADRLAEYKVLTEREAAIKRLDIIRTY
                     EQSVSKEAIAARSAAALRERMPVAELPPAITELPKWMQEMADQLDKEWASEKEIRGRQ
                     MELAGELADAEQRAAIASLEPWQRAYAQIQGDAERATREIAARYGGLPEEADKAARLT
                     TAVWAEAGLRMRDQIAGDLESAFDDITSGNIGKRFLENFKKMVFQMVATWMLGMQQMR
                     ATAGFSFSSILSTLFGIPVAGAGGVATGAATAGVAAAGGAAAGAVPAFSWAAENAYAT
                     SGALPLSTAAATQGTSLSMAGLPLSAGQGAGAGIGATLPAGAATVGAGGAALTGWPKS
                     LGKYGLPALMMGLAGASMLGGGTGSRALGGAMLGASLGAIPFALGMPMSLLLGPLAIG
                     LPVVGAVIGGLIALFSGLFGGPHKGDVARIQVMEPAQRQIKNILDEYNLFQATLDQAI
                     SQLEQLRSQGIERINQIGGNQRRGNTATLTRWVDEAENQMKTTEAERQRRLALQFGGA
                     MFHTGGYVSPSLARQTVPAWAAAAPRYQSGGAVPAVLEPREFVMSAPAVERIGRGTLE
                     RMNAGGEAGGITIHINALDPKSFSAWLRDGGMREMIRELRRARAEGAF"
     gene            12157..12969
                     /locus_tag="AC16CX_17505"
     CDS             12157..12969
                     /locus_tag="AC16CX_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014341.1"
                     /translation="MSEANILNPSATSLLNYDWGYSQGLPEVRSVWQAQSGKIYSRLQ
                     MARGRIYDLLWNNRDLATMHAFTQWEEQYRNDFFTLADWERGRYFSGRFEGPLTFNPL
                     GFEKWNIRGRFAELPGLAMYAYPSNWARDAIFYEERDDFGADLVKLTGTWIFSSSPGV
                     FHGDAAGRYYSVTTNDTAEWIFLGYGWRVWANTENDAGIMEMRVTKVRDGSVVASSNI
                     DLYSAATQPASALWTGVGIPLDLYRLRLRITGTKNPSSSNYCGFADALEVLR"
     gene            12969..>13232
                     /locus_tag="AC16CX_17510"
     CDS             12969..>13232
                     /locus_tag="AC16CX_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014342.1"
                     /translation="MRTLPAGLATELAKRGRTLQRVHLVDVERADGSRYFWSDWEINC
                     LSALTGAAQQYKPWLKEPPTIKFSRSLQADSGCFKLQNLSGNTI"
BASE COUNT         2614 a         3797 c         4870 g         1951 t
ORIGIN      
        1 caaggcgtgc cccgggcgcg tgatgggagt gaactttgcc ggcacgaacg accagggcgt
       61 gaagatcaag accgacatgg ccatgcggat gaagcagcgg tttgagaaac atctcgaccg
      121 cattcccgcg gacccggaga tccgtcaggc gctgctcgcg atcaaacgcg agacgaccgg
      181 gaccgcaatc cggttcgatg cgccgcgaat cgaggtggac tcgcccgtgg ccggtggcga
      241 aaagcggaag cgtttcggac acgccgacga attctgggcg aaggcgatgg ctagcctggc
      301 agcggcaaca ggggcgcggg tttcgacgga gttcacggcg gtgcggatga gtcaggcagc
      361 gcaactcatc agggccggtt acggacccgg cgggggccag accgcgaccc tggctgccag
      421 aggctggtga gatgggcaca gcgccaattc cgatgaggcc ggaggcgaag gaactggtca
      481 cgctcgaggt cctgcggtgg accgggctcg gcgcccaggc attctcgggc acgatgagcc
      541 cctcgacgat tttccagatg atggtacaag ggtcgccggg cgtgatgccg tactaccgtg
      601 aggtcgagga gaaggacacc gcggtgagtt cggcgctggc gatgcggcgg ttgctcgtcc
      661 tggcgcgcga tacggccatg cagccggcag atccccagaa cggccaggcg cgggaattga
      721 gcgatgcggc cgcagcgttt ctggattcga tcccgagatt cggttttgcc ctgtgggagc
      781 tgctcgacgc gccggcgtat ggttactcgg tcgtggagac gctctggcag accgatggaa
      841 gcgcggtggg ggtcgagcgg ctggtcggac ggccgcagga acttttccgg ttcgggagaa
      901 tcgccgaccc ccaggtcggc gacctggtgc tggcgaattt tcccggcggc gaaggccagc
      961 cggtgccgcc gcagaagttc ctggtgacga gttatcaccc gcggcacggc gaccggcggg
     1021 ggatgcccct gctgcgccgg ctgtactggc catcgtggtt caagcggaac gtcctgcggc
     1081 tgcacctgaa atttctggag aaagggccgg ggacggtggc ggtgaagtat ccctccgggg
     1141 cgggggacga cgaaaaaaag aaagccctcg aggcggcaga ggcgattgcc gatgagatcg
     1201 cggtggccgt gccggagact ttcgcgctga tcccggaggc cctgcagacg acgcggacac
     1261 ggcaggcgga ggacttccgg gcgctgttcg actatttcga tgcggagatg acccgaatcg
     1321 tgctgggaca gacattgacg acgcgcggcg cggagcaaca gcgcggcacg caggcgctcg
     1381 ggcaggtgca tgagaacacg atgtggcagg tggtccgcgg agacgcagcg gacctggagg
     1441 ccgcgatcaa cgagcagttg ctgggcccct ggctcctctg gaccttcggg ccgggggcgc
     1501 tcgaacgcgc ggtgcgaccc tggtggacga tcgagaagga cccgccgcgg gacgctgcag
     1561 cggcagcgga cctactggtg aaggcgcacc agcttgtcga actgccgaca agcgaggttt
     1621 acgaaatggt gcaggtgcgc gcgccggagg aaggcgagaa cgtcgtgccg cggtcggcgg
     1681 tgccggcgag tctgttccag ccgggactat gaacgcgagt tgccaattcg cgaccgggcc
     1741 ggcagtgagg ccgccgaaga tccgggtgac gcggacggcg agccgggagg cggtcgagac
     1801 gcagttggac ctcgagaaga tgctggcggc ggcggtgatg gaacaggctc gatatttcca
     1861 cgcgatcgct ggcgcactgc ttgagacggg gcgcatggca ttcgctgcgg gcgcccgagc
     1921 gatcgcggaa tcggcgggga ccggcgcgga gacgctggcg cacatcaggc ggctcgcgga
     1981 aattctcacg gccacgcgga tgcttgggcg ggtgcgtgtg cgaaaagaag ttggattgcc
     2041 gctcgcatcc gacctgcagt ttgccgaggt gctgcacggc atcggcgtgg accaggcggt
     2101 cgaatacttg agatcgctgc cggtggcgac gcgagaggaa tggctgcggt tactcgaggc
     2161 gaaccagggg ccggcgttca ctgccgccgg ggtcgagaac aaggcggcgc tggaagccct
     2221 gcgggacctg gtggggcagg cgctcgaccg cgggtggacc gccgcggagt tcgagcaagg
     2281 cgcgatggag ctgctggcga agttccagga agaagcggga gcgttgcgga cactctggaa
     2341 cacggtgacg gcaacggcga tggcgcgggg acgcgaggag atgctggacg atcccgaagt
     2401 gaagaagatc gtcgcttacc gactctacga tgccatcatc gattcgcgga cacggccgaa
     2461 ccacgcggcg ctcgacggtg gaatcgcgcc ggcggactgg gaaggctggc cggaatatgc
     2521 gccgccgaac gggttcaact gccggtgcac acttgttggg atcacggcgg tgcgggcgcg
     2581 gcggatgctc gaatcgaacg aagggttcga cctgacagtg aagatcccag tgggcgcagg
     2641 ccccgatccc ggattcaagt gaggagagcg caatggccac ggtgacgaaa aaggaagcgg
     2701 acggggaaca tcccgcgggg cactacctgg tcgtcgagga tccgcagcaa gcctccacct
     2761 ggcatctgcg agtgaagacg cctgatggga agccggacca cgggttgatg ggcgccgcct
     2821 gggccgcgct gcacgaaggc taccgcggca accggtacga ggggccgggg aaggaagagg
     2881 cgatccgaaa gctcaaggcg ctctacaagg acgaaggcat ggatatgcca gggatgaaca
     2941 tgggggactt ctcgaaccag tggatcgaaa tctttcgcgc gggcgactac ggcgcgaagg
     3001 gcaactggac tgccgaggac gtgaaacgtg tgacggcgaa cttcgagccg cagacctggc
     3061 agccgccgct cgtgctgggc cacccgaaag acgatgcacc ggcaatgggc ctggtccgcg
     3121 aactgcgcga ggaggcgggg cggctgctca tgcgggccga gaaagtgcat ccagcgctcg
     3181 aggcgctggt ccaggaaggc cgctacccga accgttcagt ggcgttctat ctcgatcccg
     3241 aggggaaagg tccggtgctg cgccacgtcg gattcctggg cgcgcagccg ccggagatca
     3301 aggggctcga accagtcaaa ttctccgatg gtgaatttgt cgccatcgag ttcaacgagg
     3361 aggaaacagt ggacccgaag gaactcagca agacggtggc ggccgagatc cgcaacttct
     3421 tctcgagcct gttcggcgag aagaagccgg atgcgacgtt caccgaggag caagtgcagg
     3481 cacggatcgc cgcggcgatg aaaccgctcg aacaggggat cgccgacctg gcgaggaagt
     3541 tcgatgaatc ggtgaagcag gcggatcagc ggacagcggc ctcgaccgaa gccgcgaaga
     3601 aggccgaggt cgctggcttc atcgagaaac aaaaagcggc gaaccgctgg gtgccggcat
     3661 tcgacgccgc cggcctgccg gccgtgctcg agcaactcgc actcaagggc ggcacggtga
     3721 agttcggcga ggcggggaaa gagaaagagg cgccggcctt cgagttgctc accggcttcc
     3781 taactgcctt gccggcgatc gtgcccacga aggaactggt gcggagcggc aaacgcatcg
     3841 gcaaggtggt cccgttcacc gagccgacca acccgagcac cgtcgtggac gccgagtcca
     3901 tggcccttgc ggaagcagcg cagcagttgc tgccggaggt gctcaaggag aacccgaaac
     3961 tcgcgcggca cgaggcatac tccgaggcgg tccgccgggc ccgcgaggcg ggagcatcca
     4021 aggcagccgg cgcgacagcc gcgggccagg cctgaattga tcgttcgaga attccgagag
     4081 aggagacaac agcatggcgt acggaacagt ggggccgcag gtgatccgca gctacaaagc
     4141 ggacgcggca atcgccgtgg gcctggccgt gatcggcggc gctgcggtgg acagcgtcgc
     4201 actgcccggc ggcgcgaacg tgcgggcgct gggcgtcacg gccgccgcgg tggatgcggc
     4261 gggcaaaccc gtgtcggtgg tcgagttcgg cgaggtcaag gccgtcgccg acgcggcgat
     4321 cgcgcggggc gactacgtga tggtaaacgc cgcgaccggc aagctggcgc cgatcggagc
     4381 cacggcgggc acgaactatc acgttgtggg catcgcgctc gaggcggcag cggcgcagaa
     4441 cgacacgttc gcgctgtttg tctgcccgtc gcgggcgcag ggctaatacc cggccaggcc
     4501 ggcaaattca gaggaggaga atcgcatggg agctttcgta agtccgattc tggcgaagat
     4561 cgacgtaccc ctgtccattt tcgcccggga gtacaggaac aacgaactga tcgccgacct
     4621 ggtcgcgccg cgcgtcgggg tgcaaaacca gtcggacaaa tactggctgt tcggccgcga
     4681 ggggcagcag atcggcggga acgtcctgcg cgggccggga tccgccgccg aacgcatcct
     4741 gcagaccatc tcgaccgatt cctattcctg ccaggaccac gctctggcgc ggttgatcac
     4801 ggatgaggag cgcgggaact tccaggcggg gaacgtcgag gaatgggcaa cgcggacggt
     4861 cgtgaaccag ttgctgctcg accgggaaaa ccgcgcggcc gcgctgttta cgacggccgc
     4921 gaattacccg accggcaaca aggtgacgct ctcgggcacg agccagtgga atgaggcgac
     4981 ccaggtctcg acgccggtga aggatgtgat gaccgcccat ctcgcggtgg cgaagatcgg
     5041 ccagaaagcg aacacgctca tcctggggcc agacgtctgg gcagccttga aggtcaacaa
     5101 gcagatcacg gaccgggtct ctccgtcgaa gttgggcgtg gtctcgaccg aagacctggc
     5161 ggcgatcttc gaggtggacc aggtgctgat cccgaagggc gtgcaagtga gcgcggcggg
     5221 cgtggcctct ttcgtctggg ggaagcacgc ggtgctggcc ttcatccagc ccggagcctc
     5281 gatggccgac gtgacattcg ccaagtcgtt cttctggacc ggggcgccag ggacgacggg
     5341 cggagtctcg acggagatcg gccgactgac gccgccaagc gcgaaggcgg acgagctcgc
     5401 ggtccacatg tactacgacc tcaagcagac gtcgaacgtt tcggcgtacc tgatcgagaa
     5461 cgcagtcgcg taaccgatcg ctgcttgagg cgggcgctcg ggccaggccg agggcgcccg
     5521 ctgacggaac ggcctggaga agggacaatc gtccatgccg aagtatctcg tgaagtcgcc
     5581 cttgcggcac gatgggcagg actacgagcc aggcgagacc gtcgacctga cgaagaaaca
     5641 ggcggaggca atgcccgcga cggtcgaacc agtcgagaag aaagccgagc cggaaaagaa
     5701 gtagggctcg atggcctatg cggccaaagc cgacctgcaa cagcgcctcg cgccggagaa
     5761 gctgacgcag ttgacggact tctccggcac cggcgaggcc gacgatgcgc ggatcgacga
     5821 ggcgttggga gcggcgagtg cggtgatcga ctcctacgcc ggcgggcggt acgcgctgcc
     5881 ccttgcagcg agcgaccagg tgcgggacct cgcgctggcg ctggcgatcc acaagctcta
     5941 tgaattccgc cagcgggagg tccccgagac ggtgaaggcc gggaaagagg cggcaatggc
     6001 cctgctcaag gacgtggcgg caggcaaggc cacgctggac cagccggcca agaaacaaac
     6061 tgtatcgagc gaggcagtga agaaggacca cacgaccgag ccggacgtct tcgacgacga
     6121 ccggttggat aaattctgat ggcggcggtc accatcagac tcgatctgaa gcgcgtgcag
     6181 cggatgctgg ggctgaccca gcaggcgctc gaacgcggcc tggtcctggc cgtggcggaa
     6241 tggctgcggc ttgcgatccg gcggagcttc gagcaggaac agagtccgga agggcgacag
     6301 tggccggcac tctcgcctgt ctatgcgcag acgaagaaac gcggccggag aatcctcgaa
     6361 cggacgggcg cgctgttcaa ccaagcggtg aacagcgtga cgatcgcggg caacgcggtc
     6421 ctggcgggcg cgtggctgcc ctacgcggcc atccaccagt acggcggagc gatcaggata
     6481 ccggagatcg tgccgcggaa gggcgcggca ttgcgctggc tggggccgag cggcgaggcc
     6541 atctttgccc ggcgggtgaa ggcccacacg gtgacgatcc cggcgcggcc gtacctgcct
     6601 tcgccagaat gggcggaact cgaatcgaag cgtgtgatcg aggagaccat ccaggacgcg
     6661 attgaccagg gcgagggaca gaaatgagca accagacact cattgccgag gcggaagacg
     6721 cgctgatcgc gctgctcaag gcgcaactcg gcggcgcaac cggggttgct gcggtgacca
     6781 gccaggactt cgacgaggcc ggcgacatca tcgcggtgcc gccggcgatc cgggtgctct
     6841 tccgcaggga gacgctcgag tcggggcgcg acgcgacggc gctgacctat gtgtcgcttg
     6901 agaattttgc ggcgctgtgt ggagcggaga acctgcggag catcgcagag gaacgcaaag
     6961 gcgcgtacga gctggtgagc caggtgtgcg acatcctggc cggcgcgcgg ctgacactgc
     7021 cgtcgaagac tgcgggccaa agaccgacgt gcatcctgcg cggcgtggag ctggtgcaat
     7081 tcggtgcgca aggcacctgg tacgccgtga gtttcgatgt ggaaagcatt tcgcagatga
     7141 gcggggtggc ggcgtaggga ggggagagat ggccagagag gatttcgtcg agatcaggct
     7201 gaccaagaag gccacggaag cggccggcgt cgggaccgtg cggatcgtga atgcgctcgt
     7261 ggatgtggag gtccgcgcag gcgagactca ccgagtcagc cgcgcagaat ggaccgagat
     7321 tttcagcctg gcgcggcttc atggcgaacc ggtgttcgag atcgcgtcca tgcccgagcc
     7381 ggtgaagcca gcgatacggc agaaagcgga gaggagctag accatggcga cgtttgcggc
     7441 gcagcaaatc ggggcgcggg aaatcgtgat cagcccgaac aagcagaacg cctacggcac
     7501 cgtgctgggc gatgtcaacc tggtgcacaa cctgcggttc gacggcggag cgttcgccga
     7561 tctggcgaaa gagtacatga ccgacatgga cctagcgaag ggccatccct gggcgacgtt
     7621 caagaatccg atccggcaga acatgcgact gtcgctcggc ggcttgcagg tctatgactt
     7681 cctggcggga tggctcgcgg cgtttctgtt cggcaaggta caaaccagcg gcgtggggcc
     7741 ttacacgcat ctgttcaccg cggacctttc gaccacgatt gctccggtga cgacggcgtg
     7801 gatccacgat accgcggacg tgaagtccaa ggtgcccgac ctggcgatca actcgttgcg
     7861 gttcaacggc gcggcggtcg ggccggtcac ggccgatgcg gagctggtcg ggtcgggccg
     7921 cttcaccgac atcgcgatgg cggcgccggc gctggcggtt ccgacgtacc tactgaacag
     7981 cgacgcggac atcctgctgg ggccgcaggg cgcgccggtg tcgatcaagg agcgcgtgcg
     8041 gagctggtca gtgaacctga cggccaacct cgaggattac cggcatcccg gcagtggact
     8101 gtatgccgga ttcacgcggc gaggcgacct gcggatcacc gtgcagctcg ggattgcggc
     8161 gaaggacgtg gacgacatcc ggacactcgg cgtgctcgac acgaagcgcg agctgcagat
     8221 caacgtaaac tccggcgcag ccgcgcaact caagttcaag tttccggcgg tgatcctgcg
     8281 ggtgaccgga ggcgccgaga ggacgttcgt cctgtggacc atcgaggcgg atgacaccgg
     8341 cgtgctgaaa gacggcgtaa acgaaccgat cgaagtcacg gcaatcaaca accaggcgac
     8401 ctacctggtt ggagcataag gcaggaaccc cggcgcgggc gcttcctcag cgcaacgcca
     8461 atttcggccg gggcagggcg cgcactgcgt ccgaactcag ggctgctgag cttgaccaca
     8521 gcgggcacgg caacaccacg cacggccccg agcctgagag acctgaaaac ttttgcggag
     8581 gtctctcatg gaagaagccc cggcaacggc cgttgggatc ccaaacgaaa cgagaaatga
     8641 cgcgctgctg ccgctggatt tggcggaacg cgcggtgacc tggtcggcgt ccggccgcgt
     8701 gcggacgcac gtctttcgcc ggctgacagc ggcggattgg gaggactata tcgcgcacat
     8761 ccggttcgag gttgagacgg gcgcgccgcc aagaatcgat gtgaccgtgg ccgggctgcg
     8821 cctgtacgaa cgtgcagtgc tcctggccga tggctacccg gtacgcggcg acgcaaaact
     8881 ggaaagcctg ccgcggtgga aggatctgct tccgatcggt gaacgcctgc aggcggtgcg
     8941 gctgctgatg caggtggatc ggcacgaggg tgacgatgcg gcgatgatcg agcccgacat
     9001 ccaaattgcg gccctcgacg ctgtctgggg caccgccggg ccggggagga cgcagaccta
     9061 tcgtggcctg cttcaccggt tcacgccgcc cacggcggag catctggccc ggtacagccg
     9121 tgaaatcagc cgggcgaaga tcatcggggg cagcaggaca gggctcaccg tctaccagcc
     9181 gcgggaaaaa atcctggtaa aactctacga cgaattgatc cagagcgtcg aaggctactc
     9241 ggtcgggggc gagcccttgg ccgatcgcga ggcgatcgtg cgggaaatgg acccgttcca
     9301 caaggcggca gcggcgagca ttattttcga cctgctgggg cgacgggacg aggaggaacc
     9361 gcaagacagg ccatgatcga ggtatggcga gacgcggaag gcgtgcggca ggcgatggcc
     9421 gagatgatgg agaacggcct gcgcgcgcgc atgcgacgaa gtttggcgca gcggatgaag
     9481 ggcgcagcga atgcggaccg cgcgttggcc ggactcgacg accagcggac gctggcggac
     9541 ggctattacg cctgggccgg gcatttgctg ctgctcgacc gcgtggtgcg acagggggtg
     9601 ctgtggaccg cgctgcgcgt cgaggaactg cgcgggctgg cggacctggg ccaggtcgag
     9661 gcggagttcg agcgggggca tccgcggtgt ccgagatgcg gcgagatgca gacggatgcg
     9721 gcagaagcgc gctgctggaa ctgcggaaag gaatttgggg ccccgaggta ggcgatggcg
     9781 acgacggcag aaatccagat cgtggtggat caggagggcg cagtccacgc cttccagcaa
     9841 atcggcaacg aagtcaagaa aatcgaaggg ccgacacggc aactgggcgg aacgagcaac
     9901 gcggtattta cccagatgcg aaaacagcag gaacaggcgc gggacgcggc gcaactgttg
     9961 tcgcgggtga tcggcgcgga ggtgccgcgc gcgctcgaga aggtgctggc gcggagccag
    10021 acgattggcc cggccctctc gaaagcgttc aatgtagccg tggtggcggc atttgcgaag
    10081 gctgccttcg atgcgatcac gcaggcggtt gaatacctgg agatcgcgct gcgatccgca
    10141 aaagaacagg cgcgactcac cgcagccgcc gggagccagg ctgaacgcga cctggtgaag
    10201 cagaccgagc aggcgatcag ggaaacgagg acacggacgc gcctactcac ggcgagcgac
    10261 ctagggaaaa tcagcgtcca gctcgcggcg gatctccaga agatcgaaga gcaacgcatg
    10321 acggccgagg cgctcgggga gacacgcctc gcggacagac ttgcggaata caaggtgttg
    10381 acggagcgcg aggctgccat caagcggctc gacatcattc ggacctacga acagtccgtc
    10441 agcaaggagg cgatcgcagc gcggagtgcg gcggcgctgc gcgagcggat gccggtggcg
    10501 gaattgccgc cagccatcac ggagttgccg aagtggatgc aggagatggc ggaccagctc
    10561 gacaaggagt gggcgagcga gaaggaaatc cgtgggcggc agatggagtt ggctggcgaa
    10621 ctcgcggatg ccgagcagcg cgcggcgatc gcatcactcg aaccctggca acgggcgtac
    10681 gcgcagatcc agggggatgc ggagcgggcg acgcgagaaa tcgccgcgag gtacggaggc
    10741 ctgccagagg aggcggacaa ggcggcgcgg ctgaccaccg cggtgtgggc ggaggcgggg
    10801 ctgcggatgc gcgaccagat cgccggagac ctcgaaagcg cgttcgacga catcacgagc
    10861 gggaacatcg ggaagagatt cctcgagaac ttcaagaaga tggtcttcca gatggtggca
    10921 acgtggatgc tcgggatgca acagatgcgc gcgaccgcag gtttttcctt cagttcgatc
    10981 ctgagcacgc tgttcgggat tccggtcgcg ggtgctggag gagttgcgac tggcgcggca
    11041 acggctggtg tggccgcggc tggcggggcc gctgccgggg cggtgccggc tttctcgtgg
    11101 gcagccgaga atgcctatgc gacgtcaggc gctttgccgc tcagcacggc cgccgcaaca
    11161 caaggaactt cgctgtcgat ggccgggctg ccgctctcgg cgggacaagg ggccggggca
    11221 ggcatcggtg cgacgttgcc ggcgggtgcg gccacagttg gcgccggagg cgccgcactg
    11281 acgggatggc ccaagagcct gggaaaatat ggactgcctg cgttgatgat gggtttggca
    11341 ggtgcgtcca tgctcggagg aggaacggga agccgggcgc tgggcggagc gatgttgggt
    11401 gcatcgctcg gcgcgatacc gttcgcgctg gggatgccca tgtccctact gcttggacca
    11461 ctcgcgatcg gattgccggt tgtcggtgct gtgatcggcg gactgatcgc gcttttcagt
    11521 gggctcttcg gcgggccgca caagggcgac gtcgcgcgga ttcaggtgat ggagccggcg
    11581 cagcgtcaga tcaagaacat cctggatgag tacaacctct tccaggcgac gcttgaccag
    11641 gcgatctcac aactcgagca actgcggtcg caggggatcg agcggatcaa ccagatcggc
    11701 gggaatcagc gccgcgggaa cacggcgacg ctgacgcgct gggtggacga ggcagagaac
    11761 cagatgaaaa ccaccgaggc ggagcgccag cgaaggctgg cgctgcaatt cggcggggcg
    11821 atgttccaca ccggcgggta cgtcagccca tcactggcgc ggcagactgt gccggcgtgg
    11881 gcggcggccg cgccgaggta ccagtcgggc ggggccgtgc cggcggtgct cgaaccaaga
    11941 gagttcgtga tgtcggcgcc cgccgtggag cggatcgggc ggggaacact cgaacggatg
    12001 aatgccggcg gcgaagcagg cgggattacg atccacatca acgcgctcga cccgaagagt
    12061 ttctcggcgt ggctgcgcga tggcggaatg agggagatga ttcgggaact gcgcagagcc
    12121 agggctgagg gggcattctg acatttcgag cttgaaatga gcgaagcaaa tattctgaac
    12181 ccgagcgcga cgagcctcct caactatgac tggggctaca gtcagggctt gccggaggtg
    12241 cggtcggtat ggcaggcgca gtcgggcaag atttactcgc ggctgcagat ggcgcgcggg
    12301 cgcatctatg acctgctgtg gaacaaccgc gatttggcga cgatgcacgc gttcacgcag
    12361 tgggaggaac agtaccggaa tgacttcttc acactcgcgg actgggaacg cgggcggtat
    12421 ttcagcgggc gattcgaggg gccgctgacg ttcaaccccc tggggttcga gaaatggaac
    12481 atccgcgggc ggttcgcgga actgccgggg ctggcgatgt acgcctatcc gagcaactgg
    12541 gcgcgcgatg cgattttcta cgaggagcgc gacgacttcg gcgcggattt ggtaaaactc
    12601 acgggaacat ggatcttttc cagctcaccg ggggtcttcc acggggacgc tgccggccga
    12661 tattactcag tcacgacgaa tgacacagcg gagtggattt ttctgggtta cggatggcgg
    12721 gtctgggcca atactgagaa cgacgccggc atcatggaga tgagggttac caaagtgcgc
    12781 gatggctcgg tagtggccag tagcaacatc gacctctaca gcgccgcaac ccagccggct
    12841 tcagcgttgt ggacgggagt cggcattccg ctggatctct atcgtctcag gttgcggatc
    12901 accggcacga agaacccgag ctcctccaac tactgtggct tcgcggatgc ccttgaggtc
    12961 cttcgctgat gcggacgctg ccggcagggt tggccaccga actcgcaaag cgcgggcgga
    13021 ccttgcagcg cgtgcacctg gtggacgtcg agcgagcgga cggttcgcgg tacttctggt
    13081 cggactggga gatcaactgc ctttcggcgc tgaccggcgc ggcacagcag tacaagccgt
    13141 ggctgaagga gccgccaacg atcaaattct cgcggtcgct tcaggccgac agcggctgtt
    13201 tcaaactaca aaacttgagc ggaaacacca tc
//
LOCUS       DBNGWZ010000562        13494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_708391, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000562 DBNGWZ010000000
VERSION     DBNGWZ010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 13494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13494
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_708391"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="AC16CX_17515"
     CDS             complement(<1..187)
                     /locus_tag="AC16CX_17515"
                     /inference="COORDINATES: protein motif:HMM:NF016326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HIR3014343.1"
                     /translation="MSVDAYSFGRIRIDGKDYDSDVLVFPGRVRDGWWRREGHSLCMD
                     DLKEVLADPPPLLVVGTG"
     gene            complement(270..1202)
                     /locus_tag="AC16CX_17520"
     CDS             complement(270..1202)
                     /locus_tag="AC16CX_17520"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014344.1"
                     /translation="MLGVLLAGVAHAQQKYPTRPVRVVVPFVAGGGVDSVARITTNGL
                     GPALGQQVIVDNRPGGRGVIGVETVSKATSDGYTLLVVSESLTIMPFVERKLAFDVRR
                     SFAPVSLLATQPHVLAIHPSVPAQSVKEFIALARARPGALSYGSGGFGQHLAGEWLKK
                     AAGFDMTHIPYKGGAQAVIDLVGGQLQAAILGQSPLLPFARSGKVRILAVSTKTRSLA
                     LPNVPTLAEAGITSVDVFQWVYMLAPANTQKEIISRLNAELTKALAIPDVKEKLQSAG
                     YEAAPSTPQQLDAMIREALERWAKLIPQLNIKPE"
     gene            1427..2383
                     /locus_tag="AC16CX_17525"
     CDS             1427..2383
                     /locus_tag="AC16CX_17525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014345.1"
                     /translation="MSIRLMPAILSILLIVPAAAFGQAFPGKTVRIVIPFPVGGPSDA
                     NARIISPYLAERWGHQVVVDSRPGGNTVIGTELVAKSAPDGHTMLLTSTGFTAAPVIQ
                     AKLPYDPIGDFVPVTMVSSSPQALVAHPSLPVKSVKELIALAKARPGQLNLANVDPSS
                     MMAGHLFTMLANVKIEAVPYKGGAPMMIDLMGGHVTLGIAAISTVQAGVRSGRVRMLG
                     VGSQEPSSLFPDAPIISKEVPGFDAVAWFGLFVPARTPKDVVARIHKDVAAGLQVPEV
                     RQRLIEIGGDPGGQSPEVFGARVRAEIARWQKVAKVVGIKPQ"
     gene            complement(2462..2695)
                     /locus_tag="AC16CX_17530"
     CDS             complement(2462..2695)
                     /locus_tag="AC16CX_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014346.1"
                     /translation="MYFLLGAWGAVLLWSVGALFAMCLAGTPFLCFFESRDRFKERQF
                     LAAAGYLLGALVAAALWLGAVGYCGYRAYLAIL"
     gene            complement(2890..3072)
                     /locus_tag="AC16CX_17535"
     CDS             complement(2890..3072)
                     /locus_tag="AC16CX_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014347.1"
                     /translation="MDQIVGFSELRWLQELTYTKRKGVMPEIIGARLLRRGLIERTNG
                     GFALTARGRIALAKLG"
     gene            complement(3096..3314)
                     /locus_tag="AC16CX_17540"
     CDS             complement(3096..3314)
                     /locus_tag="AC16CX_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014348.1"
                     /translation="MAAIQTKLLPAELESLRRLTCRTAKRIPAEHGEKLVRCGFAKNQ
                     AGTLIITTRGHAKLAFEITRASWFATPV"
     gene            complement(3609..3872)
                     /locus_tag="AC16CX_17545"
     CDS             complement(3609..3872)
                     /locus_tag="AC16CX_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR3014349.1"
                     /translation="MNIFVGNLGPDVTEAEIEELFKPFGQVVSVQLVRELFTGKLKGF
                     GFVEMPGKQHSLAAIAGLNGKELAGRPLKVNEARPRPDRGRRR"
     gene            complement(3988..4197)
                     /locus_tag="AC16CX_17550"
     CDS             complement(3988..4197)
                     /locus_tag="AC16CX_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3014350.1"
                     /translation="MSTTGTVKFFNSTKGYGFIQPDDGSKDVFVHISAVEHAGLGTLS
                     ENQKVSFEVQKGNNGKFSAINLKAV"
     gene            complement(4407..4649)
                     /locus_tag="AC16CX_17555"
     CDS             complement(4407..4649)
                     /locus_tag="AC16CX_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014351.1"
                     /translation="MNKPPAFRASLDFRGVFAQSFTSMRQPVFSIGSAVPCLKNIAVF
                     HLPDPARNGRPPDATHIRAGWPMIIGRCPVSKQANQ"
     gene            4912..5529
                     /locus_tag="AC16CX_17560"
     CDS             4912..5529
                     /locus_tag="AC16CX_17560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3014352.1"
                     /translation="MNPTSPVDSRSGIVRSISREALANLPIRRYEGKVSLVATPRELE
                     QARADIRQERVVGLDTETRPAFRKGESHLPCLVQAATAQAVYLFQLSRLDVFPALVEL
                     LAKPDIVKTGVGLAHDLRQLKLVFPFTVRNVLDLGVIARRRGLGQTGVRNLAGIFLGF
                     RIPKGNRTSNWAAPRLSPAQITYAATDAWASRELFLRFESLGLLN"
     gene            5750..6022
                     /gene="infA"
                     /locus_tag="AC16CX_17565"
     CDS             5750..6022
                     /gene="infA"
                     /locus_tag="AC16CX_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3014353.1"
                     /translation="MAKEELIELTGVVDEVLPNTVFRVTLDNGCAVTAYACGKMRQRR
                     IRILAGDRVTVEISPYDLTKGRINFRHKDTSVPPPMAPRPAYLRRR"
     gene            6035..6412
                     /locus_tag="AC16CX_17570"
     CDS             6035..6412
                     /locus_tag="AC16CX_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014354.1"
                     /translation="MKYGDIAFSGKSGHKYRFQAWSLETRFKPLAAVYFVTKRTFDNG
                     TYRRASHDSIYIGQTANLADPFATQSQFDCFRKHGANCICICLLESEEQRIAAEEDLI
                     ALHSTHCNQRQRIDRIFDAAVGA"
     gene            complement(6527..6883)
                     /locus_tag="AC16CX_17575"
     CDS             complement(6527..6883)
                     /locus_tag="AC16CX_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014355.1"
                     /translation="MITDQVMDAIAHIELHRQEMAKEAGVAAACNEALRKLHELLNLE
                     REALQSHGPGGGHAANIEAVAAEIERVKSLAGEVGKGAMPRHERAQRQKPVRPGQPHN
                     RPRTKGRRTMGRRGGR"
     gene            complement(6995..7480)
                     /locus_tag="AC16CX_17580"
     CDS             complement(6995..7480)
                     /locus_tag="AC16CX_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR3014356.1"
                     /translation="MQLLTVKIEKPETTNFILGQTHFIKTVEDVHEALVGAVPGIKFG
                     LAFCEASGACLVRCSGTDAAMLELAKKNALALGAGHTFIVFLGDGFFPVNVLNALKMV
                     PEVCRIFCATANPTEVVIAQTDQGRGILGVVDGASPKGAEAEADVAWRKNLLRQIGYK
                     L"
     gene            complement(7679..8506)
                     /locus_tag="AC16CX_17585"
     CDS             complement(7679..8506)
                     /locus_tag="AC16CX_17585"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR3014357.1"
                     /translation="MIRIAIALMSAMLLASCATAPQISPAARADLAPTGKLRVGINFQ
                     NRLLTDKDPASGEPRGIAVDLARELGRRVGVPLEIVAYDTAGKMAEAVKTGAWDVAFL
                     AIDPGRAREITFSTPYLENEATFLVPAGSPLRTIADVDREGVRIAVAAKGAPDLFLTR
                     TLKRAQLVRAPSVAAAFKLFVSDKLEVLAGYRPVLIMRAEELPGSRILDGGFSSVYQA
                     IGTPKGRDAGANYLNEFVEDVKAMGLVARLIEKNGVRGVSVAPPAQRFSTIEIGGSM"
     gene            complement(8704..9906)
                     /locus_tag="AC16CX_17590"
     CDS             complement(8704..9906)
                     /locus_tag="AC16CX_17590"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014358.1"
                     /translation="MRYEVISADCHIDLCWLPPELFTENASSAMRERMPYVSDGPDGR
                     RWTTKRGANLGLVNGMGSGGRQYVPGRIHRADRMAAMGLYEDGKRGIRRLTEPDLRVK
                     DQDRDGVQAEVLYGVLGATSRLNDTEAAVEVLRIYNEWLAGFCSTHPERFAGLASIPN
                     HPIEAAIAEVERVAGRGVLRGLDIANSPDLKPLWDPYWNPLWEVINATGLPLHFHTVS
                     GYVPEHIRKIQLIGGDPTRAVLPGMPKVELPVARAAFATNITAFQLNLSNILTSMIYS
                     GVLERHPRIRLVLGEAGIGWIPYVLWRMDAEWEDQFKDLSLTMPPSDYWKRQCWATYQ
                     TDPIGIKLLDELGADKVMWGSDFPHPDGVWPDSREYIQRELGALPAGIRRKIVCENAA
                     RLYGFPLE"
     gene            complement(9927..10892)
                     /locus_tag="AC16CX_17595"
     CDS             complement(9927..10892)
                     /locus_tag="AC16CX_17595"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014359.1"
                     /translation="MNARTCMKALALLFALLAPASHAQTYPSRPLRVLVPLSPGGGMD
                     AVTRSLAQSLTDSLGQSVVVDNRPGAGSQVALEILADAAPDGHTLMMLSATTVIYPML
                     YQSRFDVVRDFAPVSQVTAQGYALVVHPSLPAKSVLELVKYARANPGKLNYGSSGIGS
                     PIHMTTELFQIATGTQMIHVPYKGLGAAYADLVGGRIELSFATIISSQPHIRSGRLRA
                     LAVTPPKRAPALPDTPTLAEAGVPGVVVVNWYGLVAPKGTPKAILERVAAETIKAVQA
                     QELKKRLVADDSEGVGTTPAEFTAHIRAERDQWRRVIKQAGIRGQ"
     gene            complement(11055..12242)
                     /locus_tag="AC16CX_17600"
     CDS             complement(11055..12242)
                     /locus_tag="AC16CX_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014360.1"
                     /translation="MRTTTRWTGVLACGLALLAGGAAAQTIKVGLLNSTSGPFASLGD
                     QIDKSIKLYMKENAGKLPPGVKIELIVRDDGGPNPDKAKQLTQELIVRDKPDFLTGFI
                     WTPNAMSVATLLTESKTPLVIMNAAASVITERSPYIVRASFTLWGSGYYIGKWAAKRY
                     KRAYTMVTDFAPGHDSEAAFTKGFTDGGGQIIGSVRMPLKNPDYVSYMQRAKDIKPDV
                     IFNFVTAGATSTAIMKAYGDLGLREAGIPFIGTGDAVTEEELINMGDSPLGYISAWHY
                     SNAGDRPANKAFLSAWNRDYGGAVPPGFIAMAAYDAMDMIYTAIREQKGRLDPDQTMK
                     ILANYRNPNSPRGPFAIDPATRDIVHNIYIRETRKIGNRLFNVEIETIPNVKDPWKEF
                     IKK"
     gene            complement(12365..12976)
                     /locus_tag="AC16CX_17605"
     CDS             complement(12365..12976)
                     /locus_tag="AC16CX_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014361.1"
                     /translation="MAVFAVIGLVICVVSGIVFIRSKASRYKTLLLFILGLGLTSPAL
                     WFAYEIFAPDIPRDKSWDFSSSRSIAQLHEEKDVGPYYFQGNVNSTIKLPSNRQWSGT
                     AYLVVIRTRSGKIDDIHWQSRPADAPRIYEQAKRILNELGLADRDLDSWYAKVRRGEQ
                     ASFSAAARPDTDPGIEVQVRGSVKEENGWAVHVNVWWSKRNGK"
BASE COUNT         2520 a         4290 c         4127 g         2557 t
ORIGIN      
        1 aaccggtgcc tacgacaagt agcggcggcg gatcggcgag cacttccttg aggtcgtcca
       61 tacaaaggct gtggccttcg cgccgccacc agccatcgcg cacgcgcccg gggaacacga
      121 gcacgtctga gtcgtagtcc ttgccgtcga tgcggatgcg gccgaacgaa taggcgtcaa
      181 cactcatacg agtttcgact ccttcagtct cgggcgcggc tcctgttcta ttgaaaagtt
      241 acgctgaccc gattttctat tcgtcgacac tactcaggct tgatgttcaa ttgcggaatc
      301 agtttcgccc aacgctccag tgcttcacgg atcattgcgt cgagctgctg cggcgtgctg
      361 ggtgcggcct cgtatcccgc tgactgtagc ttttctttga cgtccggaat ggccagcgct
      421 ttcgtgagct ctgcgttcag acgggaaatg atttcctttt gcgtattggc aggcgcaagc
      481 atatagaccc actgaaatac gtcgacactg gtaatgcccg cctcggccag cgtcggcacg
      541 ttcggcagtg ccagtgaccg ggtctttgtg ctgaccgcga gtatcctgac ctttccactc
      601 cgcgcgaacg ggagcaatgg cgactggccg aggatcgcgg cctgcaactg accgccaacc
      661 aggtcgatga ccgcctgcgc gccgccttta taaggaatgt gtgtcatgtc gaaaccggcg
      721 gcttttttca gccattcccc cgcgaggtgt tgcccgaagc cgccgctgcc gtaggaaagt
      781 gcgccgggcc tggccctggc gagtgcaatg aattccttta cggattgtgc cggaactgaa
      841 ggatgaatcg ccagcacgtg gggttgggtt gccaggagcg aaaccggggc aaagctgcgc
      901 cgaacgtcga acgcgagttt gcgctcaacg aacggcatga tcgtgagcga ctcgcttact
      961 acgagcagcg tgtaaccgtc ggaggtggcc tttgaaaccg tttcgactcc tatcactccc
     1021 cttccgccgg gccggttatc aacgatcact tgctggccga gcgcagggcc gagcccgttt
     1081 gtggttatgc gcgccactga atcgaccccg ccacccgcga cgaacggcac caccacgcgc
     1141 acgggcctgg tcgggtactt ttgctgggcg tgcgcgacgc ccgcaagcag cactccaagc
     1201 atcaccgcta ttggccagcg aggtcgtcgc accaagtcct ccttgcaagc aaaaggagtc
     1261 aggcccccag aaaaagttac actgacccta tttgttgatg gttgcgtcat tcagcccggg
     1321 ctgtcaacat cgccccacaa tccgccgcgc gccaggcgtc gaacacggaa attggggcct
     1381 gcgccgccgg gctgccggca tctgtcaact cagccaagga ggaatcatga gcatcaggct
     1441 aatgcccgca atcctctcaa tcctgctgat cgtgcccgcc gccgcgttcg ggcaggcatt
     1501 tcccggaaag acggtgcgca tcgtgatccc gttccccgtc ggcggcccga gcgatgccaa
     1561 cgcacgcatc atcagcccgt accttgccga acgctggggg caccaggtcg tggtggactc
     1621 gcgcccgggc ggcaacaccg tgatcggcac cgagctcgtg gcaaagagcg cgcccgacgg
     1681 gcatacgatg ctgctcactt ccactggatt cacggccgcg ccggtgattc aggcgaagct
     1741 gccgtacgac cccattggcg atttcgtgcc ggtcaccatg gtttccagct ccccgcaggc
     1801 gctggtcgct cacccgtcgt tgccggtgaa gagcgtgaaa gaactgatcg cgctggcgaa
     1861 agcacggccg ggccagctca atctcgccaa cgtggacccc agctcgatga tggctggaca
     1921 tctgtttacc atgctcgcca acgtgaagat cgaggcggtg ccgtacaagg gcggcgcgcc
     1981 gatgatgatc gacttgatgg gcgggcacgt gacgctgggc atcgcggcga tcagcacggt
     2041 gcaggccggg gtgcgctcgg gccgcgtccg catgcttggc gtcggttccc aggagccctc
     2101 ttccctgttt ccggatgcgc cgatcatctc gaaggaagtg cctggcttcg acgcggtggc
     2161 gtggtttggg ctgttcgttc cggcacgcac gccgaaggac gttgtcgcgc ggatccacaa
     2221 ggacgttgcg gccggcctgc aagtgccgga ggtcaggcag cgcctgatcg agatcggcgg
     2281 cgatccgggc ggccagagcc cggaagtatt cggcgcccgc gtccgcgccg agatcgcccg
     2341 ctggcagaaa gtcgcgaagg tggtcggcat caagccacag taacgcgcgg ctcccgtact
     2401 gcggcaatca aagtggcaag acgcaaatag aaaacctagg gtcagactga ccccatttac
     2461 actaaagaat ggcgaggtac gcccggtacc cgcagtaacc caccgcaccg agccacagcg
     2521 cggcggcgac gagcgcgccc agcaggtaac ctgccgcagc gaggaactgg cgctccttga
     2581 atcggtctct gctttcgaaa aaacacaaga agggcgttcc cgccaggcac atggcgaaaa
     2641 gcgcgcccac gctccacagc agcacggccc cccacgcacc caggaggaag tacaacccgg
     2701 tgatgatcag tgcgaggagc gcggccgcga cgtagagctc gtgcaggggg aagacgcacc
     2761 tccactcaac aatgccaact cgagaaaaat gtgaaaaggt tcctgacacc ttttctgaac
     2821 gcggagaaaa tagaacggca cctgacaccc gccccgggtc cgggtctgcc acctgatgcc
     2881 cgcggctcgt caacccagct tggcaagcgc gatccggccc cgcgccgtga gcgcgaagcc
     2941 gccgttcgtg cgctcgatga gccctcgtct gaggagcctc gccccgatga tctcgggcat
     3001 cacccctttc cgcttcgtat aagtcagttc ctgcagccag cgcaactcgg aaaaaccgac
     3061 gatctgatcc atcgtccgcc ccttttcagg ccgactcaaa ccggcgtggc aaaccagctc
     3121 gcgcgggtga tttcaaacgc cagtttcgca tggcccctgg tcgtgatgat cagtgtgccg
     3181 gcttgatttt tcgcgaagcc gcagcgaacc agcttctcgc cgtgctcggc gggaatacgc
     3241 ttcgccgttc tgcaggtgag ccgacgcagc gattccagct ctgctggcag caatttcgtc
     3301 tggatggcgg ccataagtgc ccctgaagaa tcggtggacg gaggcaagcc cggctatgtt
     3361 taatcccaat ccctgaaaga tgcagccgca ggagcagccc ggaaagagac ggacccaagg
     3421 agaggaaatt aaacgcctgt ctccttatac gcggtaccag cggaaaggac aatcatcccc
     3481 ggtcacccgt gcatcctctg cgctgcgcaa cccctcgtga aatcgcgtaa tcggcaggtg
     3541 ctcgcggacg ttcccggagg tgcagaaatc gcagcgcgga ttgaatgcgt gcccgccgca
     3601 cccgacgact accggcgccg tccgcgatcg ggccggggcc gcgcctcgtt caccttcagc
     3661 gggcgcccgg cgagctcctt gccgttgagc ccggcgatcg cggcgagcga atgctgcttg
     3721 ccgggcatct ccacgaagcc aaatcccttg agcttgccgg tgaacagctc acgcacgagc
     3781 tgcaccgaca ccacctgacc gaacggcttg aacagctcct cgatttccgc ttcggtcacg
     3841 tcgggaccga ggttgccaac aaagatattc atgtcgactc ctggaagaga ccgtcgagaa
     3901 atgccgacgg cgaccgcgtc aaatgaaacc ctgcctgagc ggggtttcgc gggtgctgcg
     3961 cgccggcttt tcagccggcg cgagtcatca gacagccttc agattgatcg cggagaactt
     4021 gccattgttg cccttctgca cttcgaacga gactttctgg ttctcgctca gtgtgccgag
     4081 gcccgcgtgc tcgaccgcgg agatgtgcac gaacacgtcc ttcgagccat catcgggctg
     4141 gatgaagccg tagcccttgg tggagttgaa aaactttacg gtgccggtgg tagacataat
     4201 gtcctccata ggtggcgtgg ccgcgcgcga aagtgctcgc ggcatcaaaa taccagtggg
     4261 agaggaccgg aagtaccagc cggaaacgaa ctgcggacag acccaaagcg cattcgaccc
     4321 cggcattatg cgcccaaaac gcctgcaggc acaggacttt tcttggcgac gagtcaggcg
     4381 cgatggggct tgcaactcat cgcccatcac tggtttgctt gcttcgagac cggacaccgg
     4441 ccaataatca ttggccagcc ggcgcgaatg tgcgtagcat cggggggccg gccgttgcgg
     4501 gcagggtcgg gcaggtggaa tacggcaatg ttttttaaac agggaacggc cgaaccgatc
     4561 gagaacacag gttgacgcat cgaggtgaag gattgagcaa agacccctcg aaaatcaagc
     4621 gatgcccgga atgccggcgg cttgttcaag cgtcgaccat gcgcccgttg tctcgcacgc
     4681 ggtttggagg cggcgttcgc tatgcttgcc cggattgctt caagcgcgtt atggcgatga
     4741 ggaaggccgt aaggatggcg cgctaggaat cggcacgaga cacaatcaag accggactcg
     4801 gcccggcggg cttcgcggct caggggtaat gggctaacag cccggttacg gatcccgcgc
     4861 atcactcatc gctcgtcacc gggttagaac ccgcatcctc gatccctgca agtgaacccc
     4921 acgtcccccg tcgattcgcg gagcggaatc gttcgctcga tttcccggga ggcactggcg
     4981 aacctgccca tccgccgcta cgagggcaag gtgtccctgg tggcaacgcc gcgggagctg
     5041 gagcaggcgc gcgcggacat ccggcaggaa agggtcgtcg gcctcgatac ggagacgcgg
     5101 cccgccttca ggaagggcga gagccatctg ccctgcctgg tccaggcggc cacggcccag
     5161 gcggtgtact tgttccagct cagccgcctg gatgttttcc ccgctctcgt cgaactgctg
     5221 gcgaagccgg acatcgtcaa aacaggcgtc gggctcgcgc acgacctgcg ccagctgaag
     5281 ctggtatttc cgttcacggt gaggaacgtg ctcgacctgg gtgtcatcgc gcgccgccgc
     5341 ggcctcggcc agaccggcgt gcgcaatctc gccggaatct tcctcgggtt ccggataccc
     5401 aaaggcaacc gcacatcgaa ctgggccgcg ccgcgcctct ccccggcgca gatcacctac
     5461 gccgccacgg atgcctgggc cagccgcgaa ttgttcttgc gtttcgagag cctgggactg
     5521 ctgaattgaa ccgccgcggc gcaattctgc ttgccaaccg cgccggcagc gctcatactc
     5581 gcgcgtcgca tcggctgccg ggcagtcctc cgcattcttt ccggacggct tcgtagtctc
     5641 cccgatcgat gccgtgccca tcccgggcgc gggtctcggg aaattcacaa gcagcacatc
     5701 accgcgggat ggcggcagcg ccgccgggtc ccgccagggg aaatcgcaca tggcaaagga
     5761 agaattgatc gaattgacgg gcgtcgtcga cgaggtgctg cccaacacgg tgttccgcgt
     5821 gacgctcgac aacggctgcg ccgtcacggc ttacgcctgc ggaaagatgc gccagcgccg
     5881 catacgcatc ctggccggcg accgcgtcac cgtcgaaatc tcgccttacg acctcaccaa
     5941 gggccgcatc aacttccgcc acaaggacac cagtgtcccg ccgccgatgg cgccgcggcc
     6001 ggcgtacctg cggcgccgat agccggggga tcaaatgaaa tacggagaca tcgcgttcag
     6061 cgggaaatcg ggccacaaat accgcttcca ggcctggtca ctggaaacac ggttcaagcc
     6121 cctggcggcc gtgtacttcg tgacgaagcg cacgttcgat aacggcacct accgcagggc
     6181 gtcccatgac agcatctaca tcggccagac cgccaacctg gccgacccgt tcgccaccca
     6241 atcgcaattc gactgcttca ggaaacacgg agcgaactgt atctgcatct gcctgctgga
     6301 aagcgaggaa cagcgcatcg cggcagaaga ggacctgatt gccctgcaca gcacccactg
     6361 caaccagcgg cagagaatcg accgcatctt cgacgcggca gtaggcgctt aggggaaaaa
     6421 gtgaggagtg agcggtgacg gggttccctg tgccttaccc cccatcactc atcaccgggg
     6481 tcgtcatccc gagtagtgct gtcccctgag ggttcttgac gaacggtcaa cgtccaccgc
     6541 gccgccccat cgttctgcga cccttggtcc gggggcgatt gtgcggctgg cccggccgca
     6601 ccggcttctg ccgctgggcg cgttcgtgcc tcggcatcgc gcctttgcca acctcgccgg
     6661 ccagactctt caccctctcg atttcggcgg ccacggcttc aatgttcgcc gcatgcccgc
     6721 cgcccgggcc atgcgactgc aacgcttccc tctcgaggtt gaggagctca tgcagcttgc
     6781 gcagcgcctc gttgcacgcc gccgcgacac cggcctcctt tgccatttcc tgccggtgca
     6841 attcgatgtg ggcgatggcg tccattacct ggtcggtgat cattgctgct ctttcatcag
     6901 taatagcgct ctcaggcgac agaccaccaa aaaattgttg aagcagatca gcctttagca
     6961 atccttagcg gtcccctaag gttctcaccc ccggctagag cttgtacccg atctgccgca
     7021 gcaggttctt gcgccaggcg acgtcggctt cagcctccgc ccctttgggc gaggcgccgt
     7081 ccaccacgcc cagaatcccg cgcccctgat cggtctgcgc gatcaccact tcggtggggt
     7141 tggccgtggc gcagaagatg cggcacacct ccggcaccat cttcagcgca ttgagcacgt
     7201 tcaccggaaa gaaaccatcg cccaggaaca cgatgaacgt gtgccccgcg ccgagcgcga
     7261 gcgcgttctt cttcgccagc tccagcatgg ccgcgtcggt gcccgagcag cgcaccaggc
     7321 aggcgcctga ggcctcgcag aacgccaggc cgaacttgat gcccggcacc gccccgacga
     7381 gcgcctcatg cacgtcttcc acggtcttga tgaaatgcgt ctgcccgaga atgaagttgg
     7441 tcgtctcggg cttctcgatt ttgacggtga gcagttgcat gaccggttcc ctcctcgcac
     7501 tcttagatga cccagtttag cgcggtgaaa aaatggggtc ggagtgattt tcacaattcg
     7561 caaatttcgg atcctaaaat tgactccgac cccttttttc tcgctcgcga aaaagccgcc
     7621 gccaagcgcc cggaccgacc ctcccgaagg atgaaaccgg gtttagtttt attaatcctt
     7681 acattgaccc cccgatttct atcgtgctga agcgctgagc cgggggcgca accgacacgc
     7741 cgcggacgcc gttcttctcg atcaaccggg caacgagacc catggctttc acgtcctcga
     7801 cgaattcatt caggtagttc gccccggcgt cgcgtccttt gggcgtgcct atcgcttgat
     7861 aaaccgagct gaatccacca tcaagaatgc gcgatccagg tagttcctcg gcgcgcatga
     7921 tcagtaccgg cctgtatccg gcgaggactt ccagcttgtc tgagacgaag agcttgaacg
     7981 ctgcggcaac gctcggggcg cgcaccaact gggcacgctt caaggtacgg gtcagaaaca
     8041 ggtcaggagc accttttgcc gccacagcaa tgcgcactcc ctcgcggtcc acatcggcga
     8101 tggtgcgcag cggtgacccg gctggcacca gaaaggtggc ttcgttttcg agataggggg
     8161 tgctgaaggt gatttcgcgg gcacgccccg gatcgatcgc aaggaacgca acatcccagg
     8221 cgccggtttt taccgcctcg gccatttttc ccgcggtgtc gtaggcgacg atctcgagag
     8281 gtacgccgac ccgtctgccc agctcgcgcg ccaagtccac tgcaatgccg cggggctccc
     8341 cgctcgccgg gtctttatcc gtgagcagcc tgttttgaaa gttgattccc acgcgcagct
     8401 tgcccgtcgg cgcgagatcg gcgcgcgcgg cgggggaaat ttgtggtgct gtcgcgcacg
     8461 atgcgagcag catcgcgctc atgagcgcaa ttgcgattct gatcatggct gaattctagg
     8521 tactaaggtc ggatcagctc ttgcagctcg ctaaaatctt acactgattg aactggcccg
     8581 ggccggaagt tctcgaagaa atactgttca gccgccacga atctttcgcg atcaattcat
     8641 tgaaacacct gttcttttaa tggggtctga acccattcat tgaccccttt gaactctgcg
     8701 atgttactcc agaggaaaac cgtacaggcg ggcagcgttc tcgcacacga tcttgcggcg
     8761 gatgccggcc ggcagggcgc caagctcccg ctggatgtac tcccgcgagt ccggccagac
     8821 gccgtcggga tgcggaaagt ccgaccccca catgaccttg tccgcgccta gctcatccag
     8881 cagcttgatc ccgataggat ccgtctgata ggtcgcccag cactggcgtt tccagtagtc
     8941 gctgggcggc atcgtgagcg agaggtcctt gaactggtcc tcccactcag cgtccatccg
     9001 ccacagcaca tacgggatcc agccgatgcc cgcttcgccg agcaccagcc tgatccgcgg
     9061 gtggcgctcg aggacgccgc tgtagatcat ggatgtcagg atgttggaca ggttgagctg
     9121 aaacgccgtg atgttcgtgg cgaacgcggc ccgcgccacc ggcaactcca ccttcggcat
     9181 gccgggaagg acggcgcgcg tcggatcgcc gccgatcagc tggatcttgc ggatgtgctc
     9241 gggcacgtag ccgctgaccg tgtggaagtg cagcggcagc ccggtggcgt tgatcacctc
     9301 ccacagcggg ttccagtacg ggtcccacag cggcttgaga tccggcgagt tggcgatgtc
     9361 gagaccgcgc aggacgccgc gcccggcgac gcgctcgacc tcggcgatgg cggcctcgat
     9421 cggatggttc gggatcgagg cgaggccggc gaagcgctcg gggtgcgtcg agcagaatcc
     9481 ggcgagccac tcgttgtaga tgcgcaggac ctcgacggcg gcctcggtat cgttcaggcg
     9541 gctggtggcg ccgagcacgc cgtagagcac ctcggcctgg acgccgtcgc ggtcctggtc
     9601 ctttacgcgg aggtcgggct cggtcagacg gcggatgccg cgcttgccgt cctcgtagag
     9661 ccccatcgcc gccatgcgat cggcgcgatg gattctgccg gggacgtact gccgcccgcc
     9721 cgagcccatg ccgttcacca atccgaggtt ggcgccccgc ttcgtcgtcc agcgccggcc
     9781 gtcgggcccg tcgctgacgt acggcatgcg ctcgcgcatt gcggacgagg cgttctcggt
     9841 gaacagctcc ggcggcagcc agcagagatc tatatgacaa tccgccgaga tcacttcgta
     9901 gcgcatggcg gtgtctccgt aaccggtcat tgtccgcgga tgcccgcctg cttgatcacg
     9961 cggcgccact ggtcgcgctc ggcgcggatg tgggcggtga actccgcagg ggtggtgccc
    10021 acgccctccg agtcgtcggc aacgaggcgt ttcttcaatt cctgcgcctg caccgccttg
    10081 atcgtttcgg cggcgacccg ttcaaggatg gctttcggcg tgcccttggg cgcgacgagg
    10141 ccgtaccagt tgaccacgac gacgccgggc acgccggcct cggcgagggt aggcgtgtcg
    10201 ggcaaggccg gcgcgcgttt cggcggcgtc accgccagcg cgcgcaagcg cccgctcctg
    10261 atgtggggct gggacgagat gatggtcgca aacgacagct cgatgcgccc gccaaccagg
    10321 tccgcgtagg cggctcccag gcccttgtac gggacgtgaa tcatctgcgt gccggtggca
    10381 atctggaaca gctccgtggt catgtgaatc gggctgccga tgccggatga gccgtagttc
    10441 agcttgcccg ggtttgcgcg cgcgtatttc acgagctcga ggaccgattt cgcgggcagc
    10501 gacggatgca cgaccagtgc gtagccctgc gcggtgactt gcgacaccgg cgcgaaatca
    10561 cgcacgacgt cgaagcgcga ctggtagagc atcgggtaga tcacggtggt ggcgctgagc
    10621 atcatcaggg tatggccgtc cggcgccgcg tcggccagga tctcgagggc gacctggctg
    10681 cccgcgcccg gtcggttgtc gacgacgacg ctctgcccga gcgaatcggt aagactttgc
    10741 gcgaggctgc gcgtgacggc atccatgccg ccgcccggcg acagcggcac gagcacccgc
    10801 agcggccggc tcgggtaagt ctgggcgtgg gaagccggcg cgagcaacgc gaacagcagc
    10861 gccagcgcct tcatgcaagt gcgtgcgttc atcgggcctc ctccaggagc aaggatgaag
    10921 gatgaaggcg gaaggataaa gtaaaaactt gttaggtaga gttcttctgc cttctgcctt
    10981 ctgtgtcgcg ttggtccgac ccggacctgg gccgtttaat ggagtctgac cccgcgcccg
    11041 cgaacgcgtc cgaattattt cttgatgaat tccttccacg ggtccttgac gttcgggatt
    11101 gtctcgatct cgacattaaa gagccggttc ccgatcttgc gcgtctcgcg gatgtaaatg
    11161 ttgtgcacga tgtcgcgcgt cgccgggtcg atcgcaaacg ggccgcgcgg actgttcggg
    11221 ttccggtagt tcgcgaggat cttcatggtc tgatcgggat cgagcctgcc cttctgctcg
    11281 cggatcgccg tgtagatcat gtccatcgcg tcgtaggcgg ccatcgcgat gaacccgggt
    11341 gggacggccc cgccgtaatc gcggttccag gcggacagga acgccttgtt cgccggacgg
    11401 tcgcccgcgt tcgaatagtg ccaggccgag atatagccga gcgggctgtc acccatgttg
    11461 atcagctcct cctcggtcac cgcgtcgccg gtgccgatga aaggaatgcc ggcctcgcgc
    11521 aggccgagat cgccgtacgc cttcatgatc gcggtggagg tggcgcccgc cgtaacgaag
    11581 ttgaatatca cgtcgggctt gatgtccttc gcccgctgca tgtaggagac gtaatcggga
    11641 ttcttcagcg gcatccggac gctgccgatg atctggccgc cgccgtccgt gaaacccttg
    11701 gtgaacgccg cttcggaatc gtgtcccggc gcgaaatcgg tgaccatcgt gtaggcgcgc
    11761 ttgtaccgct tcgccgccca ctttccgatg tagtagccgc tgccccacag ggtgaacgaa
    11821 gcgcgcacga tgtagggcga gcgctccgtg atcaccgaag ccgcggcgtt catgatgacc
    11881 agcggcgtct tcgattcggt cagcagcgtc gcgaccgaca tcgcgttcgg cgtccagatg
    11941 aagccggtca ggaagtccgg cttgtcgcgg acgatcagct cctgcgtgag ctgcttcgcc
    12001 ttgtccgggt tggggccgcc gtcatcacgc acgatcagct cgatctttac gccgggcggg
    12061 agcttgccgg cattctcctt catgtagagc ttgatgctct tgtcgatctg gtcgccaagc
    12121 gaggcgaacg ggcccgatgt cgaattgagc aggccgacct tgatcgtttg cgccgccgcg
    12181 ccacccgcca gcaacgcgag cccgcaggcc agcacgccgg tccatctcgt cgttgtcctc
    12241 atgagcccct ccttatcggt gctcgatacg aacgccaatt caaaaaatgg caataaatgg
    12301 ggtcagtgta agccttcttt ttcgctccgc tgcttacctc actgaaaagt aacacagacc
    12361 ccatttattt accgtttctt ttactccacc agacattgac gtggacggcc cagccatttt
    12421 cttccttcac ggatcccctc acctgcactt caatgcccgg atcggtgtcg ggcctggcgg
    12481 cggcgctgaa tgacgcctgc tcaccgcgac gaacttttgc ataccaggaa tcaagatccc
    12541 ggtcggccag gcccagctca ttcagtatac gtttggcctg ctcgtagatt cggggggcat
    12601 cggcaggcct gctttgccag tgaatgtcgt cgatcttccc tgaccgggtt ctgatcacga
    12661 caaggtaggc tgtgcccgac cattgcctgt tggaaggtag tttgatcgtc gagttgacgt
    12721 tcccctggaa atagtacggg cccacgtcct tttcttcgtg cagctgggcg atgctgcggc
    12781 tcgaactgaa gtcccacgac ttgtcgcgcg ggatgtccgg ggcgaagatc tcgtatgcaa
    12841 accagagtgc ggggctcgtc aaccccagtc cgagaataaa tagcagcagc gttttgtacc
    12901 gggatgcctt ggaacggatg aagacgattc cactgacgac acatatcacg agccctatga
    12961 ccgcaaaaac ggccatgagg gtgatgatta tgatgcctcc agttggatcc ccgccaaggc
    13021 ctatttgagc gaacatccgg ttagcggcgg acctgagacg aggagcaact cactgaaata
    13081 aagattcttt taatggggcc tgaccctact tactctctga tcctgtttat ttgcattact
    13141 attgacagaa taccagcaga agcacatcca gggagactcc gcaaatgatg tcacgtcgcc
    13201 gtttcatcgg cagcacactt ggcgcaggcc tggcgctcgc aacgcagggg tgccagcagg
    13261 tgaccgcaca acccgcgcgt aaacgaatga tcgtagatgc tcaggtccac ctgtggaagg
    13321 cggagtcgca atagttgatg gggtcaatag ttgatggggt ctgaccccat ttatttccca
    13381 attcgaggag gactcaatga aacatgcagc tcgtctgttc ctgatttcgg ttttgtcatg
    13441 cgccgcatcg ggcgtcgccg cagcggacgc cgcggcagac tatcccagca ggcc
//
LOCUS       DBNGWZ010000563         6335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3482422, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000563 DBNGWZ010000000
VERSION     DBNGWZ010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3482422"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="AC16CX_17610"
     CDS             complement(<1..1190)
                     /locus_tag="AC16CX_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3014362.1"
                     /translation="MAVPPPIPPLPLEDVRVVEFVHMVMGPTCGLILADLGAEVIKVE
                     PLEGDHTRKLVASGAGFFPTYNRNKKSLAVDLKSREGREIVLKLIRTADVVSENFRPG
                     AMEKLGFGYQALKKVKPELIYCSHKGFLAGPYEHRTALDEVVQMMGGLAYMTGGRYGP
                     LRAGASVNDVMGGMFGAIAILAALYQRKANRKGQEVKSALFENNVFLVAQHMMEGLMT
                     GTPALPMPDRVRAWAIYDTFTTADGEQVFVGVVTDTQWKVFCEAFGCAELLADPALRT
                     NPQRVEARPRIVPIVAGIFGGLTKQQLMAKCDELGLPFAPITKPEDLFDDPHLNASGG
                     LAPITLPNGARTRVPVLPVEMDGRRFATRLDVPKLGEHTRELLGGLGYSREAVDDLVS
                     RRIVR"
     gene            1356..1709
                     /locus_tag="AC16CX_17615"
     CDS             1356..1709
                     /locus_tag="AC16CX_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3014363.1"
                     /translation="MSNGIKRHNPGPVLSRAVEYNGLVYVAGTTADNKSAPCKAQTEE
                     ILKKIDAALAAAGTSKAKLLWANVWLTDIREKEQMDAAWKAWVDPDNKPVRACVEARL
                     ATPDTRVEIMVTAAK"
     gene            1801..2880
                     /locus_tag="AC16CX_17620"
     CDS             1801..2880
                     /locus_tag="AC16CX_17620"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3014364.1"
                     /translation="MTPTPATTLARDDADAILDTVARWRAEGRGVALATVVTTWGSSP
                     RPVGSQLAADDTGAFAGSVSGGCVESAVISEARAILKGAPASLLRFGVSDEQAWEVGL
                     ACGGKVEIFVQSVVGQDSLLEVLRRVRATRKLALVATDLADGRSALVADGESAGDAAL
                     GAALADEVARALSEEQSRVVERDGRRYFLHVFAPPRRLALVGAVHIAQALIPMAVGAG
                     YEVTVIDPRSAFAAEGRFPGVTMMHEWPGTALAAFALDRRSAVVTLTHDPKLDDPALA
                     AALRSDAFYIGALGSRKTHAARLNRLREEGFGDADIARIHAPVGLPIGAVSPAEIAVS
                     ILAELTQALRGAPPLAPVRQDERTA"
     gene            complement(2981..4111)
                     /locus_tag="AC16CX_17625"
     CDS             complement(2981..4111)
                     /locus_tag="AC16CX_17625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HIR3014365.1"
                     /translation="MLATRIRQKDGIFYFASYPAKDILAKVRFISRYYGEGEQIAPQV
                     ISQSDEIAQFIARIEHSDVAFQRQLSRAKVRALKNFYEMAVNQPPIPGTVLLFTNERL
                     GFQALGSDQNVGHLQEPSSKYFIIDGQHRLAALQFYLRERPGEGNNIQVPCVIFDGRT
                     EDFAAEMFVIINSTPTRINKSHLVDLYERISWAEPDRRFAARLVDKLYREGDSPLKYR
                     INRLGGRSQQEKWILQAELFNELHRWVSGEWRKIQRTGHGMREADRYYAIVRDFLKAA
                     QKVWVNAWGHPNYMVTKPVTLKAMIRVCADLARNDADPADGRQGRWEKRLAPWTERVR
                     EFRIEGFYERFPAKGQIERVARLHRDLAKMADIEVQGRQRAG"
     gene            complement(4514..4777)
                     /locus_tag="AC16CX_17630"
     CDS             complement(4514..4777)
                     /locus_tag="AC16CX_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014366.1"
                     /translation="MKGLFLMAVAGVLLAPPLADARDWRPGFGASLQAQGQHLKKGPG
                     PVQRGDRDKGGKHDKRHQGRLTEEERRELHRDLDRANREIYRR"
     gene            5638..>6335
                     /gene="ompR"
                     /locus_tag="AC16CX_17635"
     CDS             5638..>6335
                     /gene="ompR"
                     /locus_tag="AC16CX_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmolarity response regulator transcription
                     factor OmpR"
                     /protein_id="HIR3014367.1"
                     /translation="MSELKTKILVVDDDARLRELLNRYLTEQGYAVRAVHDATEMNRQ
                     LARERYDLMILDLMLPGEDGLSVCRRLRGGGENLPIIMLTAKGDEVDRIVGLEIGADD
                     YLPKPFNPRELLARIQAVLRRRPPPAPPGAPATELQVVEFGAFRFNLAARSLTKSGEN
                     VSLTTGEFALLKVLVQHPRTPLSRDKLMELARGREFGAFDRSIDVQVSRLRKLIEPDP
                     SKPAYIQTVWGFGYV"
BASE COUNT         1057 a         2102 c         2064 g         1112 t
ORIGIN      
        1 cgcacgatcc gccgcgagac caggtcgtcc acagcttccc tgctgtagcc cagtcccccc
       61 agcaattcgc gagtgtgctc gccgagtttc ggcacgtcga gccgcgtcgc gaaacggcgt
      121 ccgtccatct ccaccggcag caccggcacc ctggtcctcg cgccattcgg cagcgtgatc
      181 ggcgcaagcc cgcccgaggc gttcaggtgc gggtcgtcga agaggtcttc cggtttggtg
      241 atcggcgcga agggcaagcc gagctcgtcg cacttcgcca tcagttgttg cttggtcagt
      301 ccgccgaaaa tgccggccac gatcggaacg atgcgcggcc gcgcctcgac acgctgtgga
      361 ttggtcctga gggcggggtc ggcgagcagc tctgcgcatc cgaacgcttc gcagaacacc
      421 ttccactggg tatcggtgac gacgcccacg aacacttgtt cgccgtccgc cgtcgtgaac
      481 gtgtcgtaga tcgcccatgc gcgcacccgg tccggcatcg gcagcgcggg cgtgcccgtc
      541 atcaaccctt ccatcatgtg ctgcgcaacc agaaacacgt tgttctcgaa cagcgcgctt
      601 ttcacttcct ggcccttgcg gttcgccttg cgctggtaca gcgcggcgag gatggcgatc
      661 gcgccgaaca tgccgcccat cacgtcgttg accgaggcgc cggcgcgcag cggaccatag
      721 cgcccgccgg tcatgtaggc gaggccgccc atcatctgca ccacctcgtc gagcgccgtg
      781 cggtgctcgt acggcccggc gaggaacccc ttgtgcgagc agtagatcaa ttccggcttc
      841 accttcttca gcgcctggta gccgaagccg agtttctcca tcgcgccagg acggaaattc
      901 tcgctcacga cgtcggccgt gcggatgagc ttgagcacga tctcgcggcc ttcgcgcgac
      961 ttgaggtcca ccgcgaggct cttcttgttg cggttgtagg tggggaagaa gcccgcgccg
     1021 gaggcgacga gcttcctcgt gtgatcaccc tcgagcggct cgaccttgat cacttcggca
     1081 ccgaggtcgg cgaggatcag cccgcaggtc ggccccatca ccatatggac gaattcgacg
     1141 acgcgcacgt cctcgagggg cagcggcgga atcggcggcg ggacggccat cggactgctc
     1201 tctgaagcgg gatttcaaac caaatgtgtt cgcaacgggc ggcgcggagc cgccgattaa
     1261 tcgtgaatgt taagggaatt ggaagccggt tccaattccc ttgcaggcgg agcaaaatcg
     1321 gtcgcgcagt gtcttccctt atcaggagca aacccatgag caacggcatc aagcgccaca
     1381 atcccggccc cgtcctgtcc cgcgccgtcg agtataacgg cctggtttac gtcgccggca
     1441 ccaccgccga caacaagagc gccccctgca aggcgcagac cgaggagatc ctgaagaaga
     1501 tcgatgccgc cctcgccgcc gccggcacca gcaaggcgaa gctgctatgg gccaacgtat
     1561 ggctcaccga catccgcgag aaggagcaga tggacgccgc gtggaaggcg tgggtggacc
     1621 cggataacaa gccggtgcgc gcctgcgtcg aggcccggct cgccaccccc gacacccgcg
     1681 tcgagatcat ggtgacagct gcgaaataga ataacccgct tccgcccgga caccgtcttc
     1741 ctgtatatat aaggatgcgg accacccgga tcacggccac cgacggtcgt gatgcgcccc
     1801 atgacgccga cacccgccac tacgcttgcg cgtgacgatg ccgatgcgat cctcgacacc
     1861 gtcgcccgct ggcgcgccga gggacgcggc gtcgctctcg ccaccgtcgt gacgacgtgg
     1921 gggtcttcgc cccgccccgt cggcagccag ctggcagcgg atgacacggg tgcgttcgcc
     1981 ggctcagtat cgggcggctg cgtggaatcc gcggtgatca gcgaggccag ggcgatcctg
     2041 aagggtgcac cggccagcct gctccggttc ggcgtctcag acgaacaggc gtgggaggtc
     2101 ggcctggcct gtggcggcaa ggtggagatc ttcgtacaat ccgtggtcgg acaagacagc
     2161 ctgcttgagg tgctgcggcg cgtccgcgcg acccggaagc tggcgctcgt cgcgaccgac
     2221 ctggcggacg ggcgcagcgc cctcgtcgcc gatggagaaa gcgcgggcga tgcggccctt
     2281 ggcgcggcgc tcgctgacga ggtggcgcgc gcgctgagcg aggaacagag tcgcgtcgtc
     2341 gagcgcgacg ggcggcgcta tttcctgcac gtcttcgcgc cgccccggcg gctcgccctc
     2401 gtcggcgcgg tgcacatcgc ccaagcgctg atcccgatgg cggtcggcgc gggctacgaa
     2461 gtcaccgtta tcgatccacg cagcgccttc gccgcggagg gccggttccc gggtgtgacg
     2521 atgatgcacg aatggcccgg caccgcgctc gccgccttcg cgctcgatcg ccgcagcgca
     2581 gtggtcacgc tcacccacga tcccaagctc gacgatcccg cgctcgccgc ggcgctgcga
     2641 tccgatgcct tctacatcgg cgcgctgggc agccgcaaga cccacgccgc gcggcttaac
     2701 cgactcaggg aagaaggctt cggcgacgcc gacatcgccc gtatacacgc cccggtcgga
     2761 ctgcccatcg gggcggtgtc gcctgccgag atcgcggtgt ccatcctcgc cgagctgacc
     2821 caggcgttgc gcggtgcgcc tccgctcgcc ccggtccgtc aggatgagag aacggcgtga
     2881 gtggtgattg gtgagtagtg agtcgaaact gttctaggat tcgtggcgta ggagtccgct
     2941 ccattcacca ttcacgattc accattcacg cattcccggt tcagccggcg cgctggcggc
     3001 cctggacctc gatgtcggcc atcttggcga ggtcgcggtg caggcgcgca acgcgctcga
     3061 tctggccctt ggcggggaag cgctcgtaga agccctcgat gcggaattcg cggactcgtt
     3121 cggtccaggg ggccagccgt ttctcccagc ggccctggcg gccatcggcg ggatcggcat
     3181 cgttgcgcgc gagatcggcg cacacccgga tcatcgcttt cagggtgacg ggcttggtga
     3241 ccatgtagtt cgggtgcccc caggcgttga cccacacctt ctgcgccgcc ttgaggaagt
     3301 cgcgcacgat ggcgtaatag cggtcggcct cgcgcatccc gtggccggtg cgctgtatct
     3361 tgcgccattc gccgctcacc cagcgatgca actcgttgaa gagctccgcc tggagtatcc
     3421 acttctcctg ctggctgcgc ccgccgaggc ggttgatgcg gtacttgagc ggactgtcgc
     3481 cctcgcggta gagcttatcc accaggcgcg ccgcgaaacg gcggtcgggc tcggcccacg
     3541 agatgcgctc gtagaggtcc accaggtggc tcttgttgat gcgcgtaggc gtggaattga
     3601 tgatgacgaa catctccgcg gcgaagtcct cggtgcggcc gtcgaagatc acgcacggca
     3661 cctggatgtt gttgccctcg ccggggcgct cccgcaggta gaactgcaat gccgccaggc
     3721 ggtgctggcc gtcgatgatg aagtatttcg agctgggctc ctgcaggtgg cccacgttct
     3781 ggtccgaccc gagcgcctgg aatccaagcc tttcgttggt gaagaggagc accgtgcccg
     3841 ggatcggcgg ctggttcacc gccatctcgt agaagttctt gagcgcgcgg accttggcgc
     3901 gcgagagctg ccgctggaac gccacgtcgc tgtgctcgat gcgggcgatg aactgggcaa
     3961 tctcgtcgct ctgggagatc acctgcggcg cgatctgctc cccctccccg taatagcggc
     4021 tgatgaagcg caccttggcg aggatgtcct tcgccggata gcttgcgaaa tagaaaatgc
     4081 cgtccttctg tcggatgcgc gtggcaagca tggccttgtt gtcccgtttt tggtccccgc
     4141 agacaaaccg gagcaccgcc tgaagttccg ggcggctgca aaaccgcatt ttagcggttt
     4201 gggagcgccg acgcgagaaa gccggcccgc cgcggggaca cgatgtggac cgggccgggt
     4261 gctcgccgga tgccggcccg gcaaaatcct gatggcaaac cgcctatcgg aaaccgggac
     4321 tcaaggcggg gcgcagtgct agcacaccgt catggctgcc gtagtggctg gcgcgcctgc
     4381 ggcgaggctc gggtccgaat ggcgtcgagt ccggcgaacg cctcgattgc tgtcgatcgt
     4441 gaatccggtt atcggagagg tctagtccgg ttccgttata gcagcctgtt gggctgccaa
     4501 cctcaacagg ctgctatcgc cggtagattt cgcggttggc ccggtccagg tcccggtgca
     4561 gctcacggcg ttcctcttcg gtgagcctgc cctgatgacg cttgtcatgc ttgcccccct
     4621 tatcgcggtc gccgcgctgg accggtccgg gtcctttctt caggtgctgc ccctgggcct
     4681 gtagcgacgc gccgaagccg ggccgccagt cgcgggcatc cgcgagcggc ggcgcgagca
     4741 acacgcccgc aacagccatc aggaacaacc ccttcaccag aacacctcca tcgtcccacc
     4801 ggcgccacct ccgggcgcag caggtttcat tataaatgcg ccataaatcc ggcggggaca
     4861 gcttgcgcag cccccgccgg tgtcacccct tacgggaagg ggatgtaaat gcattgcgcc
     4921 attgaattat ccgtatgcgc cccacgcccg ctcgagcaat gaggcatcgt atgcgtatag
     4981 aacgcatcgc gggatgcggc gctccgggtt ttttgcaaca atttgtttca gcggcacgag
     5041 gggcaacaaa cttttacaaa attctggcgc gtaagtgcct gagcggttag cattaagagc
     5101 ccgaaactta gtgaagcaca cgctgcgctg tcacggtttt ggccaggaga aaagtgcaac
     5161 attgagagtc tcaatttctg caattaactt gcaaagcttg agccaaaacc gcgccagccc
     5221 taaagggttg cggccacaat cggcggcgcg ccgaagcgtg gagcaggggc cccgcttgcg
     5281 gggatgcgac cgcgcaggcg tgcataggac cgcgattgtg gcgcgctcat gtcccgctcc
     5341 ggggggccgg tggaacgagg agcaggggcc ccgcttgcgg ggatgcgacc gtggaaccgg
     5401 ccgtgggcgc gtcgcatccg gccctatgcg cgtctccggg gtcagatccc cagaggggcc
     5461 cctcttccgc cctacgacgc gccatccaag cacaaatcgc ttggaacctg ctccgccctt
     5521 cgcgggacct ttgtcgctcg gcttgccaat atcgacatat tggctgcacc tcgcttcgcg
     5581 ccagcaccaa ttctggctcg caccgcacgc gcgcttcact aagtttcggg ctctgtcatg
     5641 agcgaattga agacgaaaat cctcgtcgtg gacgacgacg cccggctgcg cgagctgctc
     5701 aaccgctatc tcaccgagca gggctacgcg gtgcgcgcgg tgcacgacgc gaccgagatg
     5761 aaccggcagc tcgcgcgcga gcgctacgac ctgatgatcc tcgacttgat gctgccgggc
     5821 gaggacggcc tgtccgtgtg ccggcgcctg cgcggcggcg gagagaactt gccgatcatc
     5881 atgctcacgg cgaaaggcga cgaagtggac cgcatcgtgg gcctggagat cggcgccgac
     5941 gattatctgc ccaagccgtt caatccgcgg gagctgctgg cgcgcatcca ggcggtgttg
     6001 cggcggcgcc cgccgcccgc tccgcccggc gcccccgcaa ccgagcttca agtcgtggag
     6061 ttcggcgcgt tccgcttcaa tctcgcggcg cgcagcctga ccaaaagcgg cgagaatgtg
     6121 tcgctcacca ccggcgagtt cgctctcctc aaggtgctgg tgcagcaccc gcgcacgccg
     6181 ctctcgcgcg acaagctgat ggagctcgcg cgcgggcgcg aattcggcgc cttcgaccgc
     6241 tcgatcgacg tgcaggtttc gcggctgagg aagctgatcg agcccgatcc ctcgaagccc
     6301 gcttacatcc agacggtgtg ggggttcggc tacgt
//
LOCUS       DBNGWZ010000564         1813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1844168, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000564 DBNGWZ010000000
VERSION     DBNGWZ010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1844168"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="AC16CX_17640"
     CDS             complement(<1..774)
                     /locus_tag="AC16CX_17640"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014368.1"
                     /translation="MRTRILLTAFAIPLMLAVPGAQAAQSGYPDKPIRLILGSAPGSG
                     PDIIAQLIAERLYKEWGQRIVVDARPGVAGIISAEQALRANPDGYTWMMLTSQLFVAT
                     SVYPDLKFNLDKDFVSIALIGTVPFVQMVNPKVPAKSIPELIALAKKSPGKLRYGSAG
                     AGASEHLSGVLFTRLTSTDMLHVPYKGIPQAIADTLAGEVQLTYAVYPAAFPHLQGGR
                     LRALGVTTPKRSPALPDVPAIAEAVPGYAMFGWYSLVAPQ"
     gene            923..>1813
                     /locus_tag="AC16CX_17645"
     CDS             923..>1813
                     /locus_tag="AC16CX_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3014369.1"
                     /translation="MSDYKSEIRDGMRIDWDVPIKMDDGLALRADVFRPVAEGKYPAI
                     VSYGPYGKGLAFQEGYKTAWEIMCRENPDAVSGTTNQYANWEVVDPEKWVPDGYACVR
                     VDSRGAGRSPGYLDHNNARENRDFHDCIEWAAVQPWCSGKVGLNGISYYASSQWRAAA
                     LQPPHLAAICVWEGWVDYYRDSVRHGGIACTFRKHWQDMQVKTVQHGRGERGPKSRVT
                     GELMCGPETLPEEELVRNRADMWEGVLKHPLVGPYYRERTGDLSKVTVPLLSAANWGG
                     QGLHPRGNFEGFMRAASREKW"
BASE COUNT          328 a          565 c          642 g          278 t
ORIGIN      
        1 ctggggggcc accaggctgt accagccgaa catcgcatag ccgggcaccg cttcggcgat
       61 cgccgggacg tcgggcaggg ccggcgagcg cttcggggtg gtgacaccga gcgcccgcag
      121 ccttccgccc tggaggtggg gaaacgcggc cgggtatacc gcgtaggtga gctgcacttc
      181 ccccgcgagc gtgtcggcga tggcctgcgg aatgcccttg tacggcacgt gcagcatgtc
      241 ggtgctggtc aggcgcgtga acagaacgcc cgacagatgc tcggacgcgc cggcgccggc
      301 cgagccgtag cgcagcttgc cgggcgactt cttcgcgagc gcaatcaact cgggaatcga
      361 tttcgccggc accttcgggt tgaccatctg gacgaacggc accgtcccga tgagggcgat
      421 cgagacgaaa tccttgtcca ggttgaactt gaggtcggga tacaccgagg tcgccacgaa
      481 cagctgcgag gtcagcatca tccaggtgta gccgtcgggg ttcgcgcgca acgcctgctc
      541 cgcgctgatg atgccggcga cgccgggccg ggcatccacg acgatccgct ggccccactc
      601 cttgtacagc cgctcggcga tgagttgcgc gatgatgtcg ggacccgagc ccggagcgct
      661 gccgaggatg agccggatcg gcttgtccgg gtagcccgac tgggcggcct gcgcccccgg
      721 cacggccagc atgagcggga tggcgaacgc ggtcagcaga attcgagtgc gcattgttgt
      781 cctcctcttg tctcgacgac taggttaaat agaccggcgt gcggggtcaa ttgccggctt
      841 cagcgccggt tgaggcaaaa tgcgtgggag cggggtcgac cggcgatcca cggcccgcca
      901 atcaattggt tgagggagag caatgagcga ttacaagagc gaaatccgcg acggcatgcg
      961 catcgactgg gacgtgccaa tcaagatgga cgacggtctc gcgttgcgtg cggacgtgtt
     1021 ccggccggtc gcagaaggga agtatccggc gatcgtgagc tacgggccgt acggtaaggg
     1081 cctcgcgttc caggaaggct acaagacggc gtgggagatc atgtgccggg aaaacccgga
     1141 cgccgtcagc ggcacgacga accagtacgc caactgggaa gtggtagacc ccgaaaaatg
     1201 ggtgccggat ggctacgcct gcgtgcgcgt ggactcgcgc ggcgcgggcc gttcgcccgg
     1261 ttatctcgac cacaacaacg cgcgtgagaa ccgcgacttc catgactgca tcgagtgggc
     1321 cgccgtccag ccctggtgca gcggcaaggt ggggctcaac ggcatctcct attacgcgag
     1381 cagccagtgg cgcgccgcgg cgctgcagcc gccgcatctt gcggcgatct gcgtctggga
     1441 aggctgggtg gactactacc gcgattcggt ccggcacggc ggcatcgcct gcaccttccg
     1501 caagcactgg caggacatgc aagtgaaaac cgtgcagcac gggcgcggcg agcgtggccc
     1561 gaaaagccgc gtcaccggcg agctcatgtg cggccccgag acgctgcccg aagaagaact
     1621 cgtcaggaac cgtgcagaca tgtgggaggg tgtgctcaag cacccgctcg tcggacccta
     1681 ttaccgcgag cgcaccggcg acctgtcgaa agtcacggtg ccgctgctct ccgccgccaa
     1741 ctggggcggg caggggctgc acccgcgcgg caacttcgaa gggttcatgc gcgcggcgtc
     1801 gcgcgagaag tgg
//
LOCUS       DBNGWZ010000565         9238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1595226, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000565 DBNGWZ010000000
VERSION     DBNGWZ010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9238
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1595226"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="AC16CX_17650"
     CDS             <1..1404
                     /locus_tag="AC16CX_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3014370.1"
                     /translation="IHTLIGAGAASGGTLDASNLLKPALQSGQLKCIGATTYQEYRGV
                     FEKDHALSRRFQKIDIVEPSVSETIAILKGLKSRFEAHHGVKYMPSALTAAVELSARF
                     INDRYLPDKAIDVIDEAGAAQKILPKARQKRIIGKHEIEEIIAKITRVPVQTVSVDDR
                     NALKNLDRDLKAVVFGQDKAIDALSAAIKMARSGLGNPQKPIGNFLFSGPTGVGKTEV
                     ARQLAYTMGVELIRFDMSEYMERHAVSRLIGAPPGYIGFDQGGLLTEAITKHPYSVLL
                     LDEIEKAHPDIFNILLQVMDHGTLTDNNGRKADFRNVVIVMTTNAGAVELTKSSMGFT
                     PSQQAGDEMAEIRRLFTPEFRNRLDAIISFNALDREIILRVVDKFLMQLEEQLAEKKV
                     EPAFTQALREYLAKKGFDPQMGARPMARLIQDTIRSALADELLFGRLAHGGKVTVDID
                     PDEKIRLVLAEETASIA"
     gene            1587..1943
                     /locus_tag="AC16CX_17655"
     CDS             1587..1943
                     /locus_tag="AC16CX_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014371.1"
                     /translation="MKPILISATCAGALLAAGLAAPASAQDPTYARNLSAACFTCHGT
                     DGHSAGGIPPSIAGQNKVLLLQKLKDFKAGKSSATIMHQQAKGYTDQELEVIAGYFAS
                     VTAGPAAAAPATRGGY"
     gene            1960..3225
                     /locus_tag="AC16CX_17660"
     CDS             1960..3225
                     /locus_tag="AC16CX_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3014372.1"
                     /translation="MSRREFMKWMAASTGAAAVAGCADMGGPGAGRVVVIGGGFGGAT
                     AARYIKLWASDIDVTLVERNSEFVSCPMSNLVLGGNTRIENLTLGYEGLRKRGVRLLR
                     DDAVAIDAGARQVRLASGTVLQYDRLIVAPGIDFLPDAIPGLKSAEAQNRVLHAWKAG
                     PQTVALRKQLEAMRDGGVFVFHIPMAPYRCPPGPYERVCQVADYFKRAKPRSKIIVLD
                     SNPDVTSKKGLFLQSWDGVYKGMIDYRPNSELQDVDVKGMTVKLTFDNVKGDVLNVVP
                     PHGAGHIARQAGLVTANQRWCEIDWLTCESRAVKGVHVLGDATLSAPAMPKSGSMANQ
                     HGKIAAAAVVALLKGQPVFQNPAIMNACYSMVDGRSAMHVTSIHKYDPAQKTMVPVKG
                     AGGVSSKASEEEGTYAWGWARNIWADALG"
     gene            complement(3269..3649)
                     /locus_tag="AC16CX_17665"
     CDS             complement(3269..3649)
                     /locus_tag="AC16CX_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR3014373.1"
                     /translation="MSALLADLIVIVHFLFVAFVVGGLAVIWIGAAAGWYWVRNFWFR
                     VAHLAAIVFVASEAILGIWCPLTLWEARLRGVEAEKSFIAQWIHRILFYDFPEWVFTI
                     VYVLFALVVAASWWLVRPARMKRT"
     gene            complement(3710..4558)
                     /locus_tag="AC16CX_17670"
     CDS             complement(3710..4558)
                     /locus_tag="AC16CX_17670"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3014374.1"
                     /translation="MYTTLISVTDLASHLEDASWVVCDCRHDLIDTQAGRRAYLKSHI
                     RGARFVHLDEDLSALKTGRNGRHPLPAPGTLVRRLEELGISDTSQVIAYDASGGYYAA
                     RLWWMLRWLGHEAAAILDGGWDAWVKTGMATSSVIPEVKPARFVHGAALVYTVSADEI
                     THDLERRQRRLLDARSPDRYRGENETLDPVAGHIPGAVNRFFKNNLAADGCFKSAEIL
                     RQEFDALLGNTAPGQVTHYCGSGVTACHNLLAMEIAGLTGSRLYPGSWSEWCADPSRP
                     VATGAE"
     gene            complement(4603..5253)
                     /locus_tag="AC16CX_17675"
     CDS             complement(4603..5253)
                     /locus_tag="AC16CX_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3014375.1"
                     /translation="MTTAIIVFAKAPQPGAVKTRLIPLLGAKGAAALHARLVKRALET
                     ARAASFSRVELHCAPPADDPFFRFCAGRYGVAVKAQVEGDLGARMLAAFESALGGRAR
                     ALLIGSDCPALSTRHLRRAERALRDGADAVFVPCEDGGYALIGLKRAEARLFDGIAWG
                     GDGVMAETRARLTALGWRWRELETLWDVDRPEDYARLQASGLLAAGRTASSDQQSA"
     gene            complement(5329..6006)
                     /locus_tag="AC16CX_17680"
     CDS             complement(5329..6006)
                     /locus_tag="AC16CX_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR3014376.1"
                     /translation="MAITVIVPCLDEAEGIAGTLAALAPLRARGAEVIVVDGGSRDAT
                     FERAVPFADAVISAPRGRAAQMNAGAARARGEILLFLHADTWLPEGADALIVEGLKRL
                     RRGWGRFDVTIAGRHPLLRVVERLMNLRSRLTGIATGDQAIFVTRSLFTAAGGYPEIP
                     LMEDIALSKRLKRYGPPLCLKHRLTTSGRRWEERGVLRTILLMWRLRFAYWLGADPGK
                     LAMRYDR"
     gene            complement(6085..6927)
                     /locus_tag="AC16CX_17685"
     CDS             complement(6085..6927)
                     /locus_tag="AC16CX_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HIR3014377.1"
                     /translation="MSRTLITYHLSLITLLGLLALPISSAQAQFDHSHAAWTALLKKH
                     VVLIDGGKASQVRYAEFQKDRTALKAYLESLSRVSEQEFKGWSKEQQLAFLINAYNAG
                     TVEKILTRYPNIRSIWDFGKVFGHPFKDKFIRLLGKEMSLDNIEHDTIRAEGAYNDPR
                     IHFAVNCAAITCPMLREEAYVAERLDRQLEEQTVRFLSDRSRNRYNPETRALEVSKIF
                     DSFPWYGGDFRRGWKGFTSLEQFFTKYAGALASNPEHQKLVREQKVEIRFLDYDWGLN
                     DAKR"
     gene            complement(6964..9111)
                     /locus_tag="AC16CX_17690"
     CDS             complement(6964..9111)
                     /locus_tag="AC16CX_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014378.1"
                     /translation="MKKSRLALIVVIVALVAVFFAAGGHRYLTFEGIKAQQAAIEAYY
                     RTHPWRTALGYFLAYVAVTGLSLPGAAVMTLAGGAVFGLLWGTVIVSFASSIGATLAF
                     LASRFVLRDWVQSRFGPSLRALNEGVGKEGAFYLFTLRLIPAIPFFAINLAMGLTLMR
                     TWTFYWVSQLGMLAGTVVYVNAGTQLAAIESPSGILSPGLIGAFLLLGFFPLIAKKIV
                     GVVRSRKVYARWTRPVRFDRNLVVIGAGSAGLVSAYIAAAVKAKVTLIEQHRMGGDCL
                     NTGCVPSKALIKSARLLAQIQRAREFGIKSAAAEFDFADVMERVQRVIRTVEPHDSVE
                     RYTGLGVECLQGEAKITSPWTVEVKTANGIRTLATRAIVIAAGARPFVPPIPGIEDVG
                     YVTSDTVWGLRQLPKRLVVLGGGPIGCELTQCFARFGSEVTQVEMLPRVLIREDPEIS
                     QMVADRFRAEGINVLVNHRAKAFRVENGEKVLIAEHGGQDVRIAFDQLLCAVGRIANT
                     QGYGLEALGIPVTKARTVETNEYLQTLYPNIYACGDVAGPYQFTHTASHQAWYAAVNA
                     LFGRFRRFRADYSVIPWATFTDPEVARVGLNETEAKEKGIPYEVTTYGLDELDRAIAD
                     EEAHGVVKVLTVPGKDRILGATIVGEHAGDLIVEYIAAMRHGLGLKKILGTIHVYPTL
                     AEANKYAAGVWARAHAPQWLLRLAEKYLVWTRG"
BASE COUNT         1712 a         2937 c         3014 g         1575 t
ORIGIN      
        1 atccacaccc tgatcggcgc cggcgccgcc tccggcggca cgctcgatgc ctccaacctg
       61 ctgaagcccg cgctgcaatc cgggcagctg aagtgcatcg gcgccaccac ctaccaggaa
      121 taccgcggcg tgttcgagaa agaccatgcg ctctcacgcc gcttccagaa gatcgacatc
      181 gtcgagccct cggtgtcgga gaccatcgcg atactcaagg gattgaagtc gcgcttcgag
      241 gcccaccacg gcgtgaagta catgccttcc gcgctgaccg ccgcggtcga gctttcggca
      301 cgcttcatca acgaccggta cctgccggac aaggcgatcg acgtgatcga cgaagccggc
      361 gcggcgcaga agatcctgcc caaggcgcgg cagaagcgca tcatcggcaa gcacgagatc
      421 gaggagatca tcgccaagat cacgcgcgtg ccggtgcaga ccgtctcggt ggacgaccgc
      481 aacgccttga agaacctcga tcgcgacttg aaggcggtcg tgttcgggca ggacaaggcg
      541 atcgacgcgc tgtccgccgc gatcaagatg gcgcggagcg gactcggcaa cccgcagaag
      601 ccgatcggca atttcctgtt ctcggggccg accggcgtgg gcaagaccga ggtcgcgcgc
      661 cagcttgcct ataccatggg tgtggagctg atccgcttcg acatgtcgga gtacatggag
      721 cggcacgccg tgtcgcgcct gatcggggca ccgcccggat acatcggctt cgaccagggc
      781 gggctgctca ccgaggcgat caccaagcac ccgtactcgg tgctgctgct cgacgagatc
      841 gagaaggcgc acccggacat cttcaacatc ctgctgcagg tgatggacca cgggacgctc
      901 accgacaaca acgggcgcaa ggccgacttc cgcaacgtgg tgatcgtcat gaccaccaac
      961 gccggcgcgg tcgagctcac gaagtcctcc atgggtttca cgccgagcca gcaggcgggc
     1021 gacgagatgg ccgagatccg gcgcctgttc acgccggagt tccgcaaccg gctggacgcc
     1081 atcatctcgt tcaatgcact cgatcgcgag atcatcctgc gggtcgtgga caagttcctg
     1141 atgcagctgg aggagcagct cgccgagaag aaggtcgagc cggccttcac ccaggcgttg
     1201 cgcgaatatc tggcgaagaa gggcttcgat ccccagatgg gcgcgcgccc aatggcgcgg
     1261 ctgatccagg acacgatccg cagcgcgctc gccgacgagc tgctgttcgg acggctggcc
     1321 cacggcggca aggtgacggt ggacatcgac cccgacgaga agatccgcct cgtcctcgcc
     1381 gaggagaccg ccagcatcgc ctgagcctgc cccacagcac cggtcatgaa aagccccggc
     1441 cctgtgcggg gctttttgtt ggccttttcc acatgccttg acggcccctc caggcggtca
     1501 cagacttata agaaaataac cacctgctag tagcggcagc gtcgggcgcc gctatactat
     1561 tcgagccact tatcaggagg gatgccatga aaccgatctt gatcagcgca acgtgtgctg
     1621 gcgcgctgct tgcggcaggg ctcgccgcgc cggcctcggc gcaggatccg acctatgcgc
     1681 gcaatctttc cgctgcctgc ttcacctgcc acggtaccga tggccacagc gccggcggga
     1741 ttccccccag cattgccggg cagaacaagg tcttgctgct gcagaagtta aaggatttca
     1801 aggctggcaa gagctccgcg acgatcatgc accagcaggc gaagggttac acggaccagg
     1861 aacttgaggt tatcgcaggc tatttcgcga gcgtcactgc gggcccagcg gcggctgcac
     1921 cggccacgcg gggcggatac tgaggaggcg accatgaaaa taagccgacg ggagttcatg
     1981 aagtggatgg cggcctcgac cggggccgcc gcggtggccg ggtgcgccga tatgggtggt
     2041 ccgggcgccg ggcgcgtggt cgtgatcggc ggcggctttg gcggggcaac cgctgcgcgc
     2101 tacatcaagc tgtgggcgtc cgacatcgat gtcacgttgg tcgagcgcaa ttccgagttc
     2161 gtgtcctgcc cgatgagcaa tctcgtgctg ggtggcaaca cccgcatcga gaatttgacc
     2221 ctcggttatg agggattaag gaaacgcgga gtgcgtttat tgcgcgatga tgccgtggcg
     2281 atcgatgcgg gcgcgcgaca agtgcgactg gcgagcggta ccgtcctcca gtacgaccgc
     2341 ctgatcgtcg cgccggggat agactttctt cccgacgcga tcccgggttt gaagagtgcc
     2401 gaagcgcaga atcgcgtgct tcacgcctgg aaggccggac ctcagacggt ggcgctgaga
     2461 aagcagctcg aagcaatgcg cgatggcggc gtgttcgtat tccatattcc gatggccccg
     2521 tatcgctgcc cgccgggacc gtacgaacgg gtgtgccagg tcgcggacta cttcaagcgc
     2581 gcgaaaccgc gctccaagat catcgtcctc gattccaatc ccgacgtcac ttcgaagaag
     2641 ggcctgttcc tccagtcctg ggacggggtg tacaagggaa tgatcgacta ccgccccaac
     2701 agcgagctcc aggacgtgga tgtcaagggc atgaccgtga agctcacgtt cgacaacgtc
     2761 aaaggtgacg tgttgaacgt ggtgcccccg catggcgcgg gacatatcgc gcggcaggcg
     2821 gggctcgtca ccgccaacca gcgctggtgc gagatcgact ggctcacctg cgagtcgcgg
     2881 gcggtaaagg gcgtgcacgt gctgggagac gcgacgctgt ccgcgcccgc catgccgaaa
     2941 tcgggcagca tggccaacca gcacggcaag attgccgcgg ccgcggtcgt cgcgttgctg
     3001 aagggccagc ctgtcttcca gaacccggcc atcatgaacg cctgctacag catggtcgac
     3061 ggccgcagcg cgatgcacgt tacttcgatc cacaagtacg atcccgcgca gaagactatg
     3121 gttccggtta agggcgcggg cggggtgtcg agcaaggcga gcgaggaaga aggcacttac
     3181 gcttggggct gggcgcgcaa catctgggca gacgcgctcg gctgatgcgc gttcgttccg
     3241 ggaaaaggcg cgcgcatgcg cgcctttttt acgtacgttt catcctggcc ggtcgcacga
     3301 gccaccagct cgccgcgacg acgagcgcga ataaaacgta gacgatggtg aaaacccatt
     3361 cggggaagtc gtagaacaga atccggtgga tccactgcgc gatgaagctt ttttcggctt
     3421 caacaccgcg caggcgcgcc tcccaaagcg tgagcgggca ccagatgccc aggatcgcct
     3481 cgctggcgac gaacacgatc gcagcgaggt gcgcgacgcg aaaccagaag ttgcgcaccc
     3541 agtaccagcc ggcagccgcc ccgatccaga tcaccgccag ccccccgacc acgaaggcga
     3601 caaacaggaa gtggacgatc acgataagat cggcgaggag ggcgctcact taaggatgag
     3661 ggatgaggga tgaaggatga aggatgactc cagcgagctg cgctcgctgt cactcggcgc
     3721 cggtggcgac gggacgcgac gggtcggcgc accattcgct ccaggaaccg ggatacagtc
     3781 gcgagccggt gagaccggcg atttccatgg cgagcagatt gtggcaggcg gtgacgccgg
     3841 agccgcaata atgcgtcact tgaccgggag cggtatttcc gagcaacgca tcgaactctt
     3901 gccggaggat ctccgcggat ttgaaacaac cgtcggcggc gaggttgttt ttgaagaagc
     3961 gattcaccgc tcccgggatg tggcctgcca ccgggtcgag ggtttcgttt tccccgcgat
     4021 agcggtcagg gctgcgcgcg tcgagaagcc ggcgctgccg tctttccaga tcgtgcgtaa
     4081 tctcgtctgc ggacacggtg tatacgagcg cggcaccatg cacgaatcgc gccggcttga
     4141 cctccggtat taccgatgag gtggccattc cggtcttgac ccacgcatcc cagccgccat
     4201 cgaggatggc ggcggcctcg tggcccagcc agcgcagcat ccaccagagg cgagcggcgt
     4261 aatagccgcc gctcgcatca taagcaatca cctgcgacgt atcggagatc ccgagttctt
     4321 ccagccgccg cacgagcgtg ccgggcgccg ggaggggatg gcggccgttg cggccggtct
     4381 tgagcgcgga caggtcctcg tcaaggtgga cgaagcgcgc cccgcgaata tgggacttga
     4441 gataagcgcg gcgtcccgct tgagtgtcga tgagatcgtg ccggcaatcg cagacgaccc
     4501 agctcgcgtc ctcgaggtgc gacgcgaggt cggttacgga gatgagggtg gtatacatga
     4561 agctgtcagc cctcagcgtt cagccgtcag cttaatacag cttcaagctg attgctgatc
     4621 gctggaagct gtgcgcccgg cagcaagcag gccggaggcc tgcagccgcg cgtaatcttc
     4681 ggggcggtcc acgtcccaca gcgtctcgag ctcgcgccag cgccacccga gggcggtgag
     4741 ccgggcgcgt gtttccgcca tgacgccgtc ccctccccac gcaatgccgt cgaatagccg
     4801 cgcctcggca cgcttcaatc cgatcaaggc atagcccccg tcctcgcacg gtacaaacac
     4861 ggcgtcagcg ccgtcgcgaa gcgcgcgctc agcccgcctc aggtggcgcg tgctgagggc
     4921 gggacagtcg gagccgataa gcagcgcgcg cgcgcgcccg ccgagcgcgg attcgaaagc
     4981 ggcaagcatg cgcgcaccca gatcgccttc gacctgcgcc ttgacggcga caccgtagcg
     5041 gcccgcgcag aagcggaaga agggatcgtc ggcaggaggc gcgcaatgca gctcgacccg
     5101 gctgaacgag gccgcgcgcg cggtctcgag cgcgcgcttg acgagccgcg catggagcgc
     5161 ggccgcgcct ttcgccccga gcaggggaat gagccgcgtt ttgaccgcgc ccggttgcgg
     5221 agccttggca aacacgatga tggcagtggt catgtgcctt tgattatccc gtgcgttgtg
     5281 cgggagttcg tgattcagga ttttgccggg cgtcagcccg gcaaaatcct agcggtcata
     5341 ccgcatcgcg agcttgccgg gatccgcgcc gagccagtaa gcgaaccgca gccgccacat
     5401 cagcagtatg gtgcgcagca cgccgcgctc ttcccagcgg cgccccgagg tggtgaggcg
     5461 atgcttcagg cacagcggcg gcccgtaacg cttgagacgc ttggagaggg cgatgtcctc
     5521 catcagcggg atctcgggat agccccccgc cgccgtgaac agcgaccggg tcacgaagat
     5581 cgcctggtcg ccggtggcaa tgcccgtgag ccgcgaacgc aggttcatga gccgctccac
     5641 tacccgcagc agcggatgcc gcccggcgat ggtcacgtcg aaacgacccc agccgcgccg
     5701 cagccgcttg agcccctcga cgatcagcgc gtcagctcct tctggaagcc aggtgtcggc
     5761 gtgcaggaac agcaggatct cgccgcgggc gcgcgccgcg ccggcgttca tctgggcggc
     5821 gcgtccgcgc ggcgccgaga tcaccgcgtc cgcgaacggc acggcgcgct caaatgtggc
     5881 gtcgcggctg ccgccgtcca cgacgatcac ttccgcgccg cgcgcgcgca gcggcgccag
     5941 cgcggccagg gtgccggcga taccctcggc ttcgtcgaga caggggacga tgacagtaat
     6001 ggccatggta agcggcaagc ggtgagcgga aagcggagaa ttgtgaccgg cttctttatc
     6061 ggcttaccgc ttacggctta ctgctcaccg ttttgcgtcg ttgagccccc aatcatagtc
     6121 cagaaagcgg atttccacct tttgctcgcg aacgagtttc tggtgctcgg ggttgctggc
     6181 caatgcgccg gcgtacttgg tgaagaactg ctcgagcgat gtgaagccct tccagcctct
     6241 cctgaaatcg ccgccatacc atgggaagga atcgaaaatc ttggagacct cgagcgcgcg
     6301 ggtttccggg ttatagcggt tgcgcgagcg gtcggacaag aaacggaccg tctgctcctc
     6361 gagttgccgg tccaggcgct ccgcgacata ggcctcctca cgcagcatcg ggcaggtgat
     6421 cgcggcgcag ttgaccgcaa agtgaatgcg cggatcgttg tacgcgccct ccgcgcggat
     6481 ggtatcgtgc tcgatgttgt ccaggctcat ttccttgccc agcagccgga tgaacttgtc
     6541 cttgaacggg tggccgaaca ccttgccgaa gtcccagatg gacctgatgt tggggtagcg
     6601 agtgaggatt ttttcgaccg tgccagcgtt gtaggcgttg atgaggaaag cgagctgttg
     6661 ttccttgctc catcccttga attcctgttc gcttaccctg gagagcgatt cgagataggc
     6721 cttgagtgcg gtgcggtcct tctggaattc tgcataccgc acctgcgagg ccttgccgcc
     6781 gtcgatcagc acgacgtgct ttttcagcag cgcggtccac gcggcatgcg aatggtcgaa
     6841 ctgggcttgg gcgctgctga tcggcagcgc gagcaggccc agtaaagtga taagtgataa
     6901 gtgataagtg ataagtgttc tgctcatgat gctattgcgt ggccgcaatc acccgttgct
     6961 ggctcaacct ctggtccata ccaggtattt ctctgccaat cgcagcagcc attgtggagc
     7021 gtgtgcacgc gcccaaacgc cggccgcata cttgtttgct tcggccaggg tcggatagac
     7081 gtggatcgtg ccgagaattt tcttcagccc aagaccatgc cgcatcgcgg ctatgtattc
     7141 gacgatgaga tcgcccgcgt gttccccgac gatggtggcg cccagtatcc ggtctttgcc
     7201 cggcacggtc agcaccttga ccacgccgtg cgcctcctcg tcggcgatcg cgcggtcgag
     7261 ctcatccagc ccgtaggttg tcacctcgta cgggataccc ttctctttcg cctcggtctc
     7321 gttcaggccg acgcgcgcga cttccgggtc ggtaaaggtt gcccacggaa tcaccgagta
     7381 atcggcccgg aacctgcgga accggccgaa cagcgcgttc accgccgcgt accacgcctg
     7441 gtgcgaggcg gtgtgggtga actggtacgg ccccgcgacg tcgccgcagg cgtagatatt
     7501 ggggtagagg gtctgcagat actcgttggt ttccaccgtg cgcgctttcg tcaccgggat
     7561 gccgagcgct tccaggccat agccctgcgt gttggcgatg cgccccaccg cgcagagcaa
     7621 ctggtcgaat gcgatgcgca cgtcctgtcc gccatgctcg gcgatgagaa ccttctcgcc
     7681 gttttcgacc cggaacgcct tggccctgtg gttgacgagg acgttgatgc cctccgcccg
     7741 gaagcggtcc gccaccatct gtgagatctc gggatcctcg cggatcagga cgcgcggcag
     7801 catctcgacc tgggtcacct ccgagccgaa gcgcgcgaag cactgggtga gttcgcagcc
     7861 gatcggcccg ccgcccagca ccaccagccg tttcggcagc tgccgcagcc cccataccgt
     7921 gtcagaggtc acatagccca catcctcgat gccgggtatg ggcggcacga agggtcgggc
     7981 gccggccgcg atcacgatgg cgcgcgtcgc gagcgtgcgg atgccgtttg cggtcttgac
     8041 ttcgacggtc cacggcgagg tgatcttcgc ctctccttgg aggcattcca cgccgaggcc
     8101 ggtgtagcgc tccaccgagt cgtgcggctc cacggtcctg atcacccgct gcacccgctc
     8161 catcacgtcg gcaaaatcga actccgcggc tgccgacttg atcccgaact cccgcgcgcg
     8221 ctggatctgg gcgagcagcc gcgccgactt gatgagcgcc ttggagggca cgcagccggt
     8281 gttgaggcaa tcgccgccca tccggtgctg ctcgatcagc gtcactttgg ctttcaccgc
     8341 tgctgcgata taggcggata ccagacccgc agagcccgca ccgatgacga ccaggttgcg
     8401 gtcgaagcgc acgggccgcg tccatctcgc gtaaaccttg cgcgacctca ccacgcccac
     8461 gattttcttt gcgatcagcg gaaagaagcc gagcagcagg aacgccccga tcaggccggg
     8521 ggagagtatg cccgagggcg attcgatggc cgcgagttga gtgccggcgt tcacgtagac
     8581 gacggtgccg gcgagcatgc cgagctggct gacccagtaa aaggtccacg tgcgcatcag
     8641 cgtgagcccc atcgcgaggt tgatggcgaa gaaggggatc gcggggatca gccgcagcgt
     8701 gaagagatag aaggcgcctt ctttcccgac gccctcattg agggcgcgca ggctgggacc
     8761 gaaccggctt tgcacccaat cgcgcaggac aaaccgggag gcaagaaacg cgagcgtcgc
     8821 gccgatgctg gaagcgaacg agacgatcac ggtgccccac agcagcccga aaaccgcgcc
     8881 accggcgagc gtcatgaccg ccgcgcccgg cagcgacagt ccggtgacgg cgacgtatgc
     8941 caggaagtag cctagggcag tccgccaggg gtgcgtccgg tagtaagcct cgattgccgc
     9001 ctgctgcgcc ttgatgccct cgaaggtgag gtaacggtgg ccgccggcgg cgaagaacac
     9061 agcgaccaat gccacgatca ccacgatcag cgcgagcctg ctttttttca tcaagctttt
     9121 tcccctgagg atgctgctgc cttattgttg ccttgccggg agcggttgac agccgaccgc
     9181 gtggcgacgc ccgggcccgc tggccttagc agcagcctcc gccgtcgtag aaccaggt
//
LOCUS       DBNGWZ010000566         7650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_457935, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000566 DBNGWZ010000000
VERSION     DBNGWZ010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_457935"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="AC16CX_17695"
     CDS             <1..746
                     /locus_tag="AC16CX_17695"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008126 - acetylesterase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HIR3014379.1"
                     /translation="YALALMAEVALRGWRGAVVHFRGCSGELNRLPRAYHSGDSAEID
                     WVLRRLGRSNEKLFAAGVSLGGNALLKWLGETGADAHAIAEKAAALSAPVDLTAAGNA
                     LDRGLNRAYVRHFLFTMKKKALEKLKHYPGLYNAARVRAAHTLREFDDAVTAPLHGFA
                     GVDDYWTRASAKPWLDRIGVPTLMVNARDDPFLPASALPAPREVAPSVSLEQPENGGH
                     AGFVDGPFPGHLNWLPQRILEFFGADAEK"
     gene            827..2200
                     /locus_tag="AC16CX_17700"
     CDS             827..2200
                     /locus_tag="AC16CX_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003279132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR3014380.1"
                     /translation="MPIPPEIFKAYDIRGIVGRTLDAKGVELIGQAIGSEALDRGRST
                     VVIGRDGRLSGPGLSAALAAGLQKSGVDVIDVGQVATPMLYFAAHHLDTLSGVMVTGS
                     HNPPEYNGLKMVLAGETLAGEAIQQLRARIEQGKLARGAGALRTLDIREDYLKRIISD
                     VKLARPMRLVVDCGNGVAGATAPELYRRLGCEVTELYCEVDGNFPNHHPDPSRPENLE
                     DVRRALAQGKGELGLAFDGDGDRLGVVTRNGKIIYPDRQLMLFAADVLSRNPGGEVIF
                     DVKSTRHLFDWVRRHGGRPVLWKTGHSFIKRKLQETGAPLAGEMSGHFFFKERWYGFD
                     DGLYAGARLLEILSRGSDPGAMLEALPDAISTPELHLKLKEGENYALMEKLKSSARFE
                     RAREVITIDGLRVEYADGFGLARPSNTTPVIVLRFEADSEAALKRIQDDFRRALIAVK
                     PGVELPF"
     gene            complement(2197..2508)
                     /locus_tag="AC16CX_17705"
     CDS             complement(2197..2508)
                     /locus_tag="AC16CX_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HIR3014381.1"
                     /translation="MIVAIVTFGLPARRSLEEMKCVFESTAPKYLNLPGLLRKNYFVS
                     EDGLRAGGIYVWESKARAQAFYSPEWIRFVAGKYGVEPDIQYLDAPVMVDNAAGTITV
                     D"
     gene            complement(2526..4097)
                     /locus_tag="AC16CX_17710"
     CDS             complement(2526..4097)
                     /locus_tag="AC16CX_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3014382.1"
                     /translation="MFIAVFSCVSGAAFAASSQDLVKRAVDAMGGEAALAGIKTIAIR
                     GQSSTWEPEASFEPGGKPRHAGDSKFAEMRDFTTGAARFDWEHNLRAPSVRTFKLSEI
                     IAGGIGYVHGIDSNNRTRFNLSSTPPGHPMSGVRAAATLRELTRQSPRLLLDMKNDPK
                     AVKAIAAQTVDSKKLPAVQYDVRDWSFVVMFDPQTRLPARIRTLDFDPIQGDSNYDLV
                     LDDWRTVAGAKLAHSLTYKLNGRDLITVRYDQVMANPALSADLFEIPITARALAVRAA
                     MGNVPYQWVIRRTYWGTNFDSDTLGWDAGQMAEPELVDIAPGVSQTRGTTHNSVVVDM
                     GNFLVVFDAPVNELMSEWVIEAAKKRYPGKPVKYLMLTHHHWDHASGARMYVAEGATV
                     IVGKGNKEHFARMFGAPGTAANDRLQRNPRKASIIEVADQYTIKGGKREVAIYLIETQ
                     HSTGTLIGYIPDARLGFVTDLWSPGRDPLLAKPRLGEADLVKAVKKLGIMPETFAGGH
                     GSTAPFAPLLKLAGG"
     gene            complement(4188..5774)
                     /locus_tag="AC16CX_17715"
     CDS             complement(4188..5774)
                     /locus_tag="AC16CX_17715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3014383.1"
                     /translation="MKRALSLLLSSVFIGSCATMPSPQDLVGRALDAMGGADALARVK
                     TVVVKGTMKQWEPEQSFAPGGEPRFANESSFEMTGDFSRHAVRVDWVKKFAYPAPRTY
                     TFSEIATPETGYVIGVDSIGRNRQNLGTNPPAHSMSGLRLAATQRELRRSSPSLLLEM
                     RRNADKVSTSPAVTIGGVTYPAVSYQAGNFSYIVMFDPQSGLPARIRTLDYDNVWGDV
                     NYDLVLSGWRTMGGVMIAASQSYELNGKVVQDIKVTDVSTNVQIAAGTFEPPAAVKAG
                     AAKPATGNVPWQWVIRRQFIGTYMDSDNVSFDARATSSLRLVELAPGVQHQVGGTHNT
                     LVVEMRDFLVVMDAPVTDNQSNWTINAAKAKYPGKPIKYLMLTHHHMDHAGGIRAYAA
                     EGATIIVGKGAGEHFRKVLAAPFTRNPDLAARDLSRTPIIEVADKRVISDGKREVSAH
                     LTENPHAAAYLIGYVADARVAYVTDIWSPGRDPLPQKITSPLAALVMAVRKAGIAPMR
                     FAGGHGSTGDYAPLSTLAGN"
     gene            complement(5875..>7650)
                     /gene="alaS"
                     /locus_tag="AC16CX_17720"
     CDS             complement(5875..>7650)
                     /gene="alaS"
                     /locus_tag="AC16CX_17720"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041492808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR3014384.1"
                     /translation="IADHIRATSFLIVDGVIPSNEGRGYVLRRIIRRAIRHGYKLGRT
                     TPFFHSIVDDLARAMGAAYPELVKAKDRVRQVLLQEEERFAETLENGMKVLEGALTRE
                     DRMLDGETVFQLYDTFGFPVDLTADIARERGVRVDYAGFEAAMERQRERARAASKFAA
                     AAAVEYSGAATEFHGYDTLALEGKVVALYNGEGVAVQQLGGGESGIVVLDRTPFYAES
                     GGQVGDTGEIAGVAGTFAVEDTRKIQAAVFGHNGRVRTGRLRVGDKVMAAVDAPRRAR
                     TMRNHSATHLMHKALREVLGPHVQQKGSLVDAERTRFDFSHNEPMTEEQIRRVEAIVN
                     GEILANAATTARIMKFDEAARAGALAFFGDKYGDEVRVLDIGSSRELCGGTHVKRTGD
                     IGFFKVISEGGIAAGVRRIEATTGEGALAYVQSQEAMLNQAAAALKAPPQEVGQKIAQ
                     IMENVRGLEKELARFKSKLASSQGEELAGQAVEVKGVKVLAAMIDGADAKALRETMDK
                     LKDRLKPAAIVLAATDGGKVSLIAGVTADLTSKIKAGELVNYVARQVGGKGGGRPDMA
                     QAGGTDAAGLPAALASVRGWVEQKL"
BASE COUNT         1259 a         2605 c         2433 g         1353 t
ORIGIN      
        1 attacgcgct cgcgctcatg gcggaggtcg cgctgcgggg ttggcgcggg gcggtcgttc
       61 actttcgcgg ctgcagcggc gagttgaacc gcctgccgcg cgcttaccac tcgggggaca
      121 gtgccgaaat cgattgggtg ctgcggcgcc tcggccgcag caacgagaaa ctgttcgccg
      181 ccggcgtctc gctcggcggc aacgccttgc tgaaatggct cggcgaaacc ggggccgacg
      241 cacacgccat tgccgaaaag gccgcagcgc tgtcggcccc ggttgacctg acggcagcag
      301 gcaatgccct tgaccgggga ttaaaccgcg catacgtgcg ccactttctg ttcaccatga
      361 agaagaaagc gctcgaaaag ctcaagcact accccggcct atataatgcc gcgcgggtac
      421 gcgcggcaca cacgctgcgc gagttcgacg acgccgtcac cgcgccgctg cacggcttcg
      481 cgggcgtgga cgattactgg acgcgcgcga gcgccaagcc gtggctcgac cggatcgggg
      541 tgccgacgct gatggtcaat gcgcgcgacg atccgtttct gccggcgagc gcgcttcccg
      601 cgccgcgtga agtcgctccc tcggtctcgc tcgaacagcc cgagaacggc ggccatgccg
      661 ggttcgtcga cggcccgttc cccggacacc tcaattggct cccccaacgc atactggaat
      721 tcttcggcgc agacgctgag aagtaacagt tcttcgttca agatttccgg gtatagtgat
      781 cacagtagag aattgttgag acttcgcgac tccaccagcg atgccgattc cgataccacc
      841 ggaaatcttc aaggcttacg acatccgcgg catcgtcggc cgcacgctcg acgcgaaggg
      901 cgtcgagctg atcggccagg cgatcggttc cgaggctctc gaccgcggcc gctcgaccgt
      961 cgtgatcggc cgtgacgggc gcctgtccgg acccgggctt tccgccgccc tcgccgcagg
     1021 gctgcagaaa agcggcgtcg acgtgatcga cgtcggtcag gtcgcgacgc ccatgctcta
     1081 ctttgccgcc caccacctcg acaccctttc gggcgtgatg gtgaccggct cgcacaatcc
     1141 cccggagtac aacggcctca agatggtgct ggccggcgag acgctcgcgg gcgaggcaat
     1201 ccagcagttg cgggcgcgca tcgagcaggg gaaactcgcg cgcggcgcag gagcgcttcg
     1261 aacgctggac atccgtgagg attacctgaa gcgcattatt tccgacgtca agctcgcgcg
     1321 gccgatgaga ctcgtggtgg actgcggaaa cggcgtggcg ggcgccacag ctccggagct
     1381 ctaccggcgg ctcggatgcg aagtgaccga gctttactgc gaggtggacg ggaatttccc
     1441 caatcaccat ccggacccgt cccgccccga gaatctcgag gacgtccggc gcgcgctcgc
     1501 ccagggcaag ggcgaactgg gcctcgcctt cgacggcgac ggcgaccggc tcggcgtggt
     1561 cacgagaaac ggcaaaatca tctaccccga ccgccagctg atgctgttcg cggccgacgt
     1621 cctgtcacgc aaccccggtg gcgaggtgat cttcgacgtc aaatccacgc gccacctgtt
     1681 cgactgggtc aggcgccacg gcgggcgccc cgtgctgtgg aaaaccggcc actcgttcat
     1741 caagcggaaa ttgcaggaaa ccggcgcgcc gctcgcgggc gaaatgagcg ggcacttttt
     1801 cttcaaggag cgctggtacg ggttcgacga cgggctctac gcgggcgcgc ggctgctcga
     1861 gatcctgtcg cgcgggagcg acccgggtgc gatgctggag gccctgccgg acgccatcag
     1921 cacgccggag ctgcacctga agctcaagga gggcgagaac tacgcgctga tggaaaagct
     1981 caaaagcagc gcgcgcttcg agcgcgcgcg cgaggtcatc accatagacg ggctgcgggt
     2041 ggaatacgcc gacggcttcg ggctggcgcg cccctccaat accacgcccg tcatcgtgct
     2101 gcgtttcgaa gcggacagcg aagccgccct aaagcgcatt caggacgatt tccgccgggc
     2161 gctaatcgcc gtcaagccgg gcgtcgaatt gcctttctag tcgaccgtaa tcgttcccgc
     2221 ggcgttgtcc accatcaccg gcgcgtccag gtactggatg tccggctcaa cgccatactt
     2281 gccggcgacg aaacgtatcc actccgggga atagaacgcc tgcgcgcgcg ccttcgactc
     2341 ccagacgtag atgcccccag cgcgtaggcc gtcctcgctg acgaagtagt tcttgcgcag
     2401 cagcccgggc aggttcaggt acttgggcgc cgtactctcg aacacgcact tcatttcctc
     2461 aagcgagcgc cgcgctggca gcccgaacgt gacgatagcg acgatcatcg aggcagctca
     2521 gggatctagc ctccggcgag cttgagcagc ggtgcgaacg gggctgtgct gccgtgcccg
     2581 ccggcaaaag tctcgggcat gatacccagc ttcttcacgg ccttcaccag gtcggcttcc
     2641 ccgagcctcg gcttggcaag cagcggatcg cgccccggcg accacaagtc ggtgacgaaa
     2701 ccgagcctgg catccgggat gtacccgatc aacgtaccgg tcgagtgctg ggtctcgatg
     2761 aggtagatcg cgacctcgcg cttgccgccc ttgatcgtgt actggtcggc cacttcgatg
     2821 atgctcgcct tgcgcgggtt gcgctgcagc cggtcgttcg cagcggttcc cggcgcgccg
     2881 aacatgcgcg cgaaatgctc cttgttgccc ttgccgacga tgacggtcgc gccctccgcg
     2941 acatacatgc gcgcgccgct ggcgtgatcc caatggtgat gcgtgagcat caggtacttg
     3001 accggcttgc cggggtagcg cttttttgcc gcctcgatca cccactccga catcagctcg
     3061 ttgacgggcg cgtcaaacac caccaggaag ttgcccatat ccaccacgac ggagttgtgc
     3121 gtggtaccgc gcgtctggct cactcccggc gcgatatcaa ccagttccgg ctccgccatt
     3181 tgcccggcgt cccagcccag cgtgtcggag tcgaagttgg tgccccagta ggtccggcgg
     3241 atgacccact gatagggcac attgcccatc gccgcgcgta ctgcaagtgc ccgcgcggtg
     3301 atcgggatct caaaaagatc ggcactgagc gcggggttgg ccatcacctg gtcgtacctg
     3361 accgtgatga gatcgcgccc gttgagcttg taggtgaggc tgtgcgcgag cttcgcgccc
     3421 gccaccgtcc gccagtcgtc cagcaccaag tcgtagttgg agtcgccctg gattgggtcg
     3481 aaatcgagcg tccggatgcg ggcgggtagg cgcgtctgcg gatcgaacat gacgacgaag
     3541 ctccagtcgc gcacgtcgta ctgcaccgcc ggcagcttct tgctgtccac cgtctgcgcg
     3601 gcgatggcct tcaccgcttt gggatcgttt ttcatgtcga gcagcaggcg cggcgactgg
     3661 cgcgtgagct cacgcagcgt ggcggcggcg cgcacccccg acatcggatg cccgggcgga
     3721 gtgctggaca ggttgaacct ggtgcggttg ttggaatcga tgccgtgcac gtagccgatg
     3781 ccgcccgcga tgatctcgct caacttgaag gtgcgtacgc tcggcgcccg gagattgtgc
     3841 tcccagtcaa accgcgcggc gccggtcgtg aaatcccgca tttccgcgaa cttggagtcg
     3901 ccggcgtggc gcggcttgcc gccgggctca aaagacgcct cgggctccca ggtgctgctc
     3961 tggccgcgga tcgcgatggt cttgatcccc gccagcgcgg cctcgccccc cattgcatcg
     4021 accgcgcgct tgacgagatc ctgggatgac gccgcaaacg cagcgccgga aacgcacgag
     4081 aaaaccgcga tgaacaagac acggaacgga atatttatgt tctcctccta cggcagaaaa
     4141 cacagccgcc ggggatcggc cccgggtgag tttcgtgaaa gcagcggtca gttgcccgcg
     4201 agcgtcgaaa gtggcgcgta gtcccccgtc gagccgtggc caccggcgaa ccgcatcggc
     4261 gcgatgccgg ccttcctcac ggccataacc agtgccgcga gcggcgaggt aatcttctgc
     4321 ggcagcgggt cgcggccagg gctccagata tcggtgacgt atgccaccct cgcgtccgcc
     4381 acgtagccga tgaggtaagc ggcggcatgc ggattctcgg tgaggtgcgc ggaaacctcg
     4441 cgcttgccat cggagatcac gcgcttgtct gccacctcga tgatcggggt cctgctcagg
     4501 tcgcgcgcgg cgagatcggg gttgcgggtg aagggtgccg ccagtacctt gcgaaaatgt
     4561 tcccccgcgc ccttgccgac gattatcgtt gcgccctccg cggcgtatgc gcggatcccc
     4621 ccggcgtggt ccatatgatg atgggtcagc atcaggtact tgatcggctt gccgggatac
     4681 ttcgccttcg ccgcgttgat cgtccagttc gactggttat ccgtcacggg cgcgtccatc
     4741 accaccagaa agtcgcgcat ctcgacgacg agggtgttgt gcgtcccgcc cacctggtgc
     4801 tgcacgcccg gcgcgagctc gaccaatcgt aagctcgaag tcgcgcgtgc atcgaagctg
     4861 acattgtcgg aatccatgta ggtaccgatg aactggcgcc ggatcaccca ctgccagggc
     4921 acgttgccgg tcgcgggctt cgccgcgccc gctttcaccg ccgcgggcgg ctcgaaagtg
     4981 ccggctgcga tctgcacgtt cgtgctcacg tccgtgacct tgatgtcctg cacgaccttg
     5041 ccattcagct cgtagctctg gcttgcggcg atcatcacgc cgcccatcgt gcgccagccc
     5101 gaaagcacca ggtcgtaatt cacgtcgccc cagacgttgt cgtaatcgag cgtgcggatg
     5161 cgcgccggca gcccgctctg gggatcgaac atgacgatgt aggaaaaatt acccgcctgg
     5221 tagctcaccg ccgggtaggt gacgccgccg atcgtgacgg ccgggctcgt cgacaccttg
     5281 tcggcattcc gccgcatctc aagcagcagc gagggcgagg agcgcctcag ctcgcgctgg
     5341 gtcgctgcaa gccgcagccc cgacatggag tgcgcgggtg ggttcgtccc gaggttctgc
     5401 cggttgcgcc cgatgctgtc cacgccgatg acgtatccgg tttcgggcgt ggcgatctcg
     5461 ctgaaggtat aggtgcgcgg tgccggatac gcgaatttct tcacccagtc cacccgcacc
     5521 gcgtgccggg agaaatctcc cgtcatttca aagctcgatt cgttcgcgaa ccgcggttcg
     5581 ccacccggcg caaacgactg ctccggctcc cactgcttca tcgtgccttt gacgacgact
     5641 gtctttaccc tggccagcgc atcagcgccg cccatcgcgt caagcgcgcg gccgacgaga
     5701 tcctgcggcg agggcatcgt cgcacaggaa ccgatgaaaa cggacgacag aagcaacgat
     5761 agagcgcgtt tcatgacccc ctcccttttc gtggaatgaa ccgcggaacc tgcggcgagc
     5821 cgcagggtgc tgaagtatac acttcccgcc ccgggaaaat tccagggggc ggtcttacag
     5881 tttctgctcg acccagcccc ggacggaagc aagcgccgcc ggcagcccgg ccgcgtcggt
     5941 accccccgcc tgagccatgt cgggacgccc cccgcccttg ccccccacct gccgcgcgac
     6001 gtaattcacc agctcccccg ccttgatttt gctggtgaga tcggcggtga caccggcgat
     6061 cagcgaaacc ttgccgccat cggttgcggc gagcacgatg gcggccggct tgagccggtc
     6121 tttcagcttg tccatggtct cgcgcagcgc cttggcgtcg gcgccatcga tcatcgccgc
     6181 gagtaccttg actcccttca cttcgaccgc ctgcccggcc agctcctcac cctgcgatga
     6241 ggcgagcttc gatttgaatc gcgccagctc tttttccagc cccctcacgt tctccatgat
     6301 ctgcgcgatc ttctgcccca cttcctgcgg cggcgccttc agcgcggcgg cggcctggtt
     6361 gagcatcgct tcctggcttt gcacgtaggc aagcgcgccc tcgccggtcg tcgcctcgat
     6421 gcggcgcacg ccggcggcga tgccgccctc ggagatcacc ttgaagaagc cgatgtcgcc
     6481 ggtgcgtttc acatgcgtgc cgccacacag ctcgcgcgag gagccgatgt cgagcacacg
     6541 cacttcgtcg ccatacttgt cgccgaaaaa ggcgagcgcg ccggcccttg ccgcctcgtc
     6601 gaacttcata atgcgcgccg tggtcgcggc attggcgagg atctcgccgt tgacgattgc
     6661 ctcgacccgc cggatctgct cctccgtcat cggttcgttg tgcgagaagt cgaagcgcgt
     6721 gcgctccgcg tccaccagcg atcccttctg ctgcacgtgc gggcccagca cctcacgcag
     6781 cgccttgtgc atgaggtggg tcgccgagtg gttgcgcatg gtgcgcgcgc ggcgcggcgc
     6841 gtccactgca gccatcacct tgtcgccgac acggagccgc ccggtcctca cgcggccgtt
     6901 atggccgaac acggcggcct ggatcttccg cgtgtcctcg accgcgaagg tgcccgcaac
     6961 tcctgcgatc tcgccggtgt ccccgacctg gccgcccgac tcggcgtaga acggcgtgcg
     7021 gtcgagtacc acgattcccg actcgccccc gccgagctgc tggacggcaa cgccctcgcc
     7081 gttgtagagc gcgacgacct tcccttcgag cgcgagcgtg tcgtagccgt gaaactcggt
     7141 ggcggcgccg ctgtactcga cggccgccgc cgccgcgaat ttcgaggccg cgcgtgcccg
     7201 ctcgcgctgg cgctccatcg ccgcctcgaa gcccgcgtag tccacgcgca cgccgcgctc
     7261 gcgcgcaatg tcggcggtaa gatccaccgg aaagccgaag gtgtcgtaga gctggaacac
     7321 ggtctcgcca tcgagcatcc ggtcctcgcg cgtcaacgcc ccttcgagca ccttcatgcc
     7381 gttctccagc gtctccgcga agcgctcctc ctcttgcagc aggacctggc gcacccggtc
     7441 tttcgccttc acgagctcgg ggtatgcggc gcccatcgcg cgcgcgagat cgtccacgat
     7501 gctgtggaag aacggcgtgg tccgcccgag cttgtagccg tggcggatcg cgcgacggat
     7561 gatgcgccgg agcacgtagc cgcggccctc gttcgacggg atcacaccgt ccacgatcag
     7621 gaaggatgtc gcgcggatgt ggtccgcgat
//
LOCUS       DBNGWZ010000567         6637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3106755, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000567 DBNGWZ010000000
VERSION     DBNGWZ010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3106755"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="AC16CX_17725"
     CDS             complement(<1..1222)
                     /locus_tag="AC16CX_17725"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014385.1"
                     /translation="MSAADITGRLARYMAQARERSLAPHVAREAKHRILDTLAAMVSG
                     AHLKPGEMAIRYARAQGGVAEATILTTDISTSAVNAALANGMFAHADETDDFEPVTKA
                     HPGCSVVPAALAMAEREGSSGMELLRAVTLGYDLCCRFLLALGPDLVRATHRSAEGTG
                     STMGSVAAAASLARLDEKGMRHALSYAAQQVSGVWSWVRDAEHVEKAFDFSGMGARNG
                     VTAAIMAQMGFSGVWDVFDGEHNALQALSTDPTPEAMVAGLGSRYFVTETAIKPFSVG
                     YPIQAPLDAFLRLHREHGLSVDNVERIVVKLPEDGARIVNDRSMPDVNCQHIIAVALM
                     DGTVSFEDSHSYERMADPKVRAAKERVQLVADRELMDPAAPRSGLVEVTLRDGRTVSH
                     FTRHAPGTKENPLDT"
     gene            complement(1401..1895)
                     /locus_tag="AC16CX_17730"
     CDS             complement(1401..1895)
                     /locus_tag="AC16CX_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR3014386.1"
                     /translation="MPDPVLYKLAEEVGTELKRRKLMLATAESCTGGWISEAVTMVPG
                     SSDWFERGFVTYTYISKREMLGVEVATLDKHGAVSEQVVREMVTGALARSHAQVAVAV
                     SGVAGPSGGTPDKPVGMVCFAWCMKDGKPLTETKRCSGDREEVRRQSVVHALKGVLAL
                     LARQ"
     gene            complement(2004..2498)
                     /locus_tag="AC16CX_17735"
     CDS             complement(2004..2498)
                     /locus_tag="AC16CX_17735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR3014387.1"
                     /translation="MADLVLRPSWRFVLRHPAHFVAFGFGAGLVPAAPGTFGTLLALP
                     LYWLLFLRMEAVEFLLLVLALFSLGIWACDVTGRAIGVSDHGGMVWDETVAFLLVLFF
                     VPATFPWQAAAFVLFRLFDILKPPPIRYYERTFRNGFGVMLDDLVAAFYTLIVLAVAT
                     RLLD"
     gene            complement(2526..3353)
                     /locus_tag="AC16CX_17740"
     CDS             complement(2526..3353)
                     /locus_tag="AC16CX_17740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase family protein"
                     /protein_id="HIR3014388.1"
                     /translation="MDADGKPLGTEFLDLEAYLKRIGYAGRLQPTYAVLEALHLAHAT
                     HIPFENLDILLGRPISLDLASLQAKLVAGGRGGYCFEHNLLFAAVLEKLGFGVRRLAA
                     RVRLRAKKPLPRTHMLLLLEVEGARWLADVGFGGEGLLLPVPFDAGRETRHFAWTYRV
                     AKERPGLWALQSLQQGAWTDLYAFSLEPQSLADYEMANFYTSTHPESLFVSTLTAQLP
                     TPEVRHILRNRELITDRGEIVERRTLGGDDELIEVLARIFGLRFPAGTRFRYRDNMS"
     gene            complement(3381..4577)
                     /locus_tag="AC16CX_17745"
     CDS             complement(3381..4577)
                     /locus_tag="AC16CX_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR3014389.1"
                     /translation="MNSGKELPILIAGGGIGGLSAALALALKGRRVHVLEKAPEFGEI
                     GYGIQMGPNVSRMLDRLGLLKALEPHSVFPDALIMVDAVENREISRITLGTKFLERYR
                     YRYFVIHRRDLHGEIYEACKRREEITLEASRGLLGFEERGGVVKVRCENGAEYEGAAL
                     IGAEGLWSPTRAAIVGDGAPRVSGHVTYRGVVPTGEIIDRSHLDSMTIWVGPSFHLVQ
                     YRLRGGKVMNNVATVSSPRFRRGEKKDFGGWNELEEAVSRAAPPVRDMLRYFQRDRNW
                     VLHDRDPVTNWTRGRATLLGDAAHPTLQYLAQGANMAIEDGVVLAEKVAAAGDDHPRA
                     FLAYQRERMNRTARVVLSSRFFGEYFHVDGGARELRNELARGRDPENPWEIDWLYRGI
                     EVDGRL"
     gene            complement(4612..5577)
                     /gene="thiL"
                     /locus_tag="AC16CX_17750"
     CDS             complement(4612..5577)
                     /gene="thiL"
                     /locus_tag="AC16CX_17750"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR3014390.1"
                     /translation="MALSEFEIIRRFFTRPARSAVLGVGDDAAIVRARRGRELVVTTD
                     MLIAGRHFRAGADPEKLGHKALAVNLSDLAAMGATPRWTTLALALPRADARWLAAFSR
                     GFMRLARRHRVDLIGGDTTRGPLNISVAVIGDLPAGTALRRDGARAGDDVWVSGTLGD
                     AALALAARGLGLARRERARVERRLDLPTPRIALGGRLRGIARSAIDVSDGLLADLGHI
                     CERSRVAAQLNLDRIPASAMLRPRLARAAARTALLAGGDDYELVFTAGRARREDIRRL
                     SRALRLKLTRVGKITPRRKGAPLVAVFDSSGRPIRVGRGGYEHFQ"
     gene            complement(5644..5943)
                     /locus_tag="AC16CX_17755"
     CDS             complement(5644..5943)
                     /locus_tag="AC16CX_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014391.1"
                     /translation="MDERLKKRIMMFYLAGVINAFLGLYVVIEGRAFLPPEIVRWLAL
                     FFFAFAAVDFWFPRLLKKKWMEAQTRLDSQRQAAPRRDEGGGMRDEGKAAGGGKL"
     gene            complement(6013..6444)
                     /gene="nusB"
                     /locus_tag="AC16CX_17760"
     CDS             complement(6013..6444)
                     /gene="nusB"
                     /locus_tag="AC16CX_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3014392.1"
                     /translation="MKTNRRRSREFALQGLYQWQLAKTDPATIMRQLAEATGFDKIDA
                     DYFKALLEGSIAAAPELEKVITPHLDREFSRLSPVERGILLMAAYELMHRPEVPYRAV
                     INEAVELAKSYGGTDGYKFVNGVLDKLAAQLRATEHVSGKQ"
     gene            complement(6441..>6637)
                     /locus_tag="AC16CX_17765"
     CDS             complement(6441..>6637)
                     /locus_tag="AC16CX_17765"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3014393.1"
                     /translation="VVANESAGGITTVALDTGVPVANGILTTDDDDQAAERMSEKGAD
                     CALAAIEMANLLKKLDEVHG"
BASE COUNT         1146 a         2294 c         2116 g         1081 t
ORIGIN      
        1 ccgtatccag cggattttcc ttcgtgcccg gggcgtggcg cgtaaagtgg ctgacggtac
       61 ggccatcgcg gagcgtcact tccaccaggc cgctccgcgg cgcggcggga tccatgagct
      121 cccggtcagc gacgagctgc acgcgctcct tcgcagcgcg tactttcggg tccgccatcc
      181 gctcatagga atggctgtcc tcgaacgaga cggtgccgtc catcagggct accgcgatga
      241 tgtgctggca gttgacatcc ggcatggagc ggtcgttgac gatgcgggcg ccatcctccg
      301 ggagcttcac gacgatccgt tccacgttgt caacgctcag cccgtgctcg cgatggagcc
      361 tgaggaacgc gtccaaaggc gcctggatcg gatagccgac ggagaaaggc ttgatggcgg
      421 tttcggtgac gaaatagcgg cttcccagtc ccgccaccat tgcctccggt gtcgggtcgg
      481 tggacagtgc ctggagcgcg ttgtgctcgc catcgaacac gtcccacaca ccgctgaagc
      541 ccatctgcgc catgatggcc gcggtgacgc cgttgcgggc gcccatgccg gagaagtcga
      601 aggccttctc gacgtgctcg gcatcgcgca cccagctcca cacgcccgaa acctgctgag
      661 cagcgtagga gagcgcgtgg cgcatcccct tttcatccag ccgggccaat gaggccgcgg
      721 ccgccacgct gcccatcgtg gagccggtgc cctcggcgct gcggtgggtt gcgcgcacga
      781 ggtccggtcc gagtgccagg agaaaacggc agcagaggtc gtagccgagc gtgaccgcgc
      841 gcagcagctc cattcccgaa ctgccctcgc gctccgccat cgcgagggcc gcgggcacga
      901 ccgagcagcc gggatgggct ttcgtcaccg gctcgaagtc atcggtctcg tcggcgtggg
      961 cgaacatgcc gttggccaga gcggcgttta cggcggaagt actgatatcg gtggtgagga
     1021 tggtcgcttc cgcgacgccg ccctgcgccc gcgcgtagcg gatcgccatc tccccgggtt
     1081 tgagatgggc tcccgaaacc atcgcggcca gcgtgtccag aatgcgatgc ttcgcttccc
     1141 gcgccacgtg tggcgcgaga ctccgctccc gcgcctgcgc catgtagcgc gccagccgcc
     1201 cggtgatatc agccgcagac attcaaaccc cttatttcgt gatgcgtgat gcgtgaatcg
     1261 tgattcgacc cttcaacctg gtaagccgtg atccgacaca cgaccttcgc agcaggttct
     1321 gacggctgac ggctggcggc tgacgactga cggctgccaa cttccaatcc ctgaggactt
     1381 agcactcact cgtcactgca tcactggcgc gccagcagcg ccagcacgcc cttgagcgca
     1441 tgcaccaccg actgccgccg cacttcctcg cggtcgccgg agcaacgctt cgtttccgtg
     1501 agcggcttgc cgtccttcat gcaccacgcg aaacacacca tgccgaccgg cttgtcaggc
     1561 gtcccgccac tggggccggc gacgccgctc actgcgaccg cgacctgcgc gtggctgcgc
     1621 gcaagcgcgc ccgtgaccat ctcgcgcacg acctgctcgg atacggcgcc gtgcttatcg
     1681 agcgttgcta cctcgacgcc cagcatctct cgcttggaga tataggtgta ggtgacgaaa
     1741 ccgcgctcga accagtcgga gctgcccggc accatggtga ccgcttccga aatccagccg
     1801 ccggtgcacg actccgccgt cgcaagcatg agcttgcgac gctttaactc ggtgcctacc
     1861 tcttctgcca gcttgtatag gacggggtcg ggcatcggat tccctaaacg catgataggt
     1921 gacgagtgat aagtgctgca agcgcaaagc caaattctcg gtaactcctg tcacttatca
     1981 cctatcactt atcacgctct ttttcaatcg agcaatctcg ttgccaccgc cagcacgatg
     2041 agcgtataga acgcggcgac cagatcgtcg agcatcacgc cgaagccgtt cctgaacgtg
     2101 cgctcgtaat agcggatcgg cggtggcttg aggatatcaa acagccggaa cagcacgaac
     2161 gccgccgcct gccaggggaa cgtcgccggc acgaagaaca acaccagcag gaacgccacc
     2221 gtctcgtccc acaccatgcc gccgtgatcg gagacgccga tcgcccgccc agtcacatcg
     2281 caggcccaga tgccgaggct gaacagcgcc aggaccagca gcagaaactc gaccgcctcc
     2341 atccgcagga agagcagcca gtagagcggc agcgcgagca gcgtgccgaa cgtgcccggc
     2401 gcggcaggta cgagaccggc gccgaagccg aacgcaacga aatgcgccgg atggcgcagc
     2461 acgaagcgcc aggaggggcg caggacgagg tcagccacgt gtgacaggac cacggggaac
     2521 cggcatcagg acatgttatc ccgatagcgg aagcgtgtcc cggcgggaaa ccgcagtccg
     2581 aatatcctcg ccagcacctc gatcagctcg tcgtcgcccc cgagcgtgcg gcgctcgacg
     2641 atttcgcccc ggtcggtgat gagctcgcga ttgcggagga tgtgccgcac ctccggcgtg
     2701 ggcagctggg cggtcagcgt gctcacgaac agcgattccg gatgcgtcga agtgtagaag
     2761 ttcgccattt cgtaatcggc gagactttgc ggctccagcg agaacgcata gaggtcggtc
     2821 caggcgcctt gctgcagcga ctgcagcgcc cacaggcccg gcctctcctt ggccacccgg
     2881 taggtccagg cgaagtgccg cgtctcgcgt ccggcgtcga acggcaccgg cagcagcagc
     2941 ccttcgccgc cgaagcccac gtccgccagc cagcgcgcgc cctcgacctc gagcagtagc
     3001 agcatgtgcg tccggggaag gggttttttg gcgcgaaggc gcacccgggc cgcgagccgc
     3061 ctgacgccga acccgagttt ttcgagcacg gcagcgaata gaaggttgtg ctcgaagcag
     3121 tagccgccgc gtccgcccgc gacgagcttc gcctgcaggc tggcaaggtc gaggctgatg
     3181 ggccgcccca gcaggatgtc gagattctcg aacgggatgt gcgtcgcgtg cgcgagatgc
     3241 agcgcctcca acaccgcata agtcggctgc agcctgccgg cgtacccgat gcgctttagg
     3301 taggcttcga gatcgaggaa ctcggtgccc aacggttttc catcggcgtc catcggcgtt
     3361 tatcggcggt tgcgtttagg tcacaagcgt ccgtccacct cgatgccgcg gtagagccag
     3421 tcgatctccc acgggttctc cgggtcgcgg ccgcgcgcca gctcgtttct cagctcgcgc
     3481 gcgccgccgt cgacgtggaa atactcgccg aagaaacgcg aggagagcac cacgcgcgcg
     3541 gtgcggttca ttcgctcgcg ctggtacgca aggaatgcgc gcgggtggtc gtctcccgcc
     3601 gcggccacct tctcggccag caccacgccg tcctcgatcg ccatgttcgc gccctgcgcg
     3661 aggtactgca gcgtcgggtg cgcggcgtca cccagcagcg tcgcccgccc gcgtgtccag
     3721 ttggtcaccg gatcacgatc gtgcagcacc cagttgcggt cacgctggaa ataacgcaac
     3781 atgtcccgca ccgggggcgc ggcccgcgaa accgcctcct ccagctcgtt ccacccgccg
     3841 aagtccttct tctcgccgcg gcgaaagcgc gggctggaga cggtcgcgac attgttcatc
     3901 accttgcccc cccgaagacg atactgcacg aggtggaagc tcgggccgac ccagatcgtc
     3961 atcgagtcga ggtggctgcg gtcgatgatc tcgcccgtcg gcaccacgcc gcggtaggtc
     4021 acgtggcccg acacgcgggg ggcgccgtcg cccacgatcg ccgcgcgcgt gggcgaccag
     4081 aggccctcgg cgccgatcag cgccgcccct tcgtattcgg cgccgttctc gcagcgcact
     4141 ttcacgacgc cgccgcgctc ctcgaagccc agcaggccgc gcgaggcctc gagggtgatt
     4201 tcctcgcgcc gcttgcaggc ttcgtagatc tccccgtgca gatcgcggcg gtgaatgacg
     4261 aaataacggt atcgatagcg ctccaggaat ttcgtgccga gcgttatcct cgagatctcc
     4321 cggttttcga ccgcatcgac catgatcagc gcgtcgggaa agacggaatg cggttcgagc
     4381 gccttcagca ggccgagccg gtccagcatg cggctcacgt tcgggcccat ctggatgccg
     4441 tagccgatct cgccgaactc cggtgccttc tccagcacgt gcacgcgccg ccccttcagc
     4501 gccagcgcga gtgcggcgga aagcccgccg atgccgccgc cggcgatcag gatcggaagt
     4561 tccttgccag aattcatgtc aaattcagga atcggacagg acttgcactg tctattggaa
     4621 gtgttcgtaa ccgccgcgcc cgacgcggat cggccgcccg ctcgagtcga aaacggcgac
     4681 cagcggcgcc cccttgcgcc gcggcgtgat tttaccgact cgcgtgagtt tgaggcgcag
     4741 cgcccgggac agccggcgga tgtcctcgcg ccgcgcgcgg ccggccgtga acacgagctc
     4801 gtaatcgtcg ccgccggcga gcagcgccgt ccgggccgcg gcacgtgcaa ggcgcggcct
     4861 cagcatggcc gatgccggta tgcgatcgag attgagctgc gccgccacgc gcgagcgctc
     4921 gcagatgtgg ccgaggtccg caaggagtcc gtcggaaacg tcgatcgcgc tgcgcgcgat
     4981 accacgcagc cgcccgccca gcgcgatgcg cggcgtcggc agatcgagcc tccgctccac
     5041 gcgcgcgcgc tcccggcgcg cgaggcccag gccgcgcgcg gcaagcgcca gggctgcatc
     5101 gccgagtgtc ccggataccc agacatcgtc gcccgcgcgg gccccgtcgc ggcgcagcgc
     5161 cgtcccagcc ggcaaatcgc cgatgaccgc aacgctgatg ttgaggggcc cgcgcgtcgt
     5221 atccccgccg atcaggtcca cgcggtgacg ccgcgcgaga cgcatgaagc cgcgcgagaa
     5281 cgccgcgagc cagcgcgcgt cggcccgcgg cagcgcgagc gccagcgtcg tccagcgcgg
     5341 ggtcgcgccc atcgccgcca ggtcggaaag gttgacggcg agcgccttgt ggccgagctt
     5401 ctccgggtcg gcgcccgcgc ggaaatgccg cccggcgatg agcatgtcgg tcgtgacgac
     5461 gagttcccgt ccgcgccgcg cccgcacgat cgccgcgtcg tcgcccacgc ccagcacggc
     5521 gctacgcgcc ggccgggtga agaaccgcct gatgatctcg aactcggaga gggccatgaa
     5581 ccgggtgacg aagtgatgca gtgatgaagt gacggggaaa ctcgtgagag ggcgaaggat
     5641 aacctaaagc tttccgcctc ctgccgcctt tccctcatcc ctcatccctc cgccctcatc
     5701 cctccgcggc gccgcctggc gctgcgagtc cagcctcgtc tgcgcttcca tccatttttt
     5761 cttgagcagg cggggaaacc agaaatcgac ggcggcgaac gcgaagaaga aaagcgccag
     5821 ccagcgcacg atctcgggcg gcaggaaagc ccgtccctcg atcacgacgt agagcccgag
     5881 gaaggcgttg atgacgcccg cgaggtagaa catcatgatc cgcttcttga gacgctcgtc
     5941 catcacagac cccgaaccaa atggtaagca gcaagcggtt agcagtaagc agaaaagcgg
     6001 ctcatttccc gctcactgct taccgctcac gtgctcggtg gcgcgcagct gcgccgcgag
     6061 cttgtcgagc acgccgttca cgaatttgta gccgtcagtg ccgccgtagc ttttcgccag
     6121 ctcgaccgcc tcgttgatga ccgcgcgata cggcacctcg ggacggtgca tgagctcgta
     6181 tgccgccatc agcagaatgc cgcgctcgac cgggctcaag cgggaaaact cgcgatcgag
     6241 atgcggcgtg atcaccttct ccagctcggg cgccgcggcg atcgagcctt ccagcagcgc
     6301 cttgaagtag tcggcgtcga tcttgtcgaa acccgtcgct tccgccagtt gccgcatgat
     6361 cgtagccgga tcggtctttg cgagctgcca ctggtacaac ccctgcagcg cgaactcgcg
     6421 cgagcggcgg cgattggtct tcatccgtga acttcgtcga gcttcttgag aagattggcc
     6481 atctcgatcg ccgccagagc gcagtcggca cccttctccg acatgcgctc agccgcctgg
     6541 tcgtcgtcgt ccgtggtcag gatcccgttg gcgaccggca cgccggtgtc gagcgccacc
     6601 gtggtgatgc cgcccgccga ctcgttggcg accacct
//
LOCUS       DBNGWZ010000568         6260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_335016, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000568 DBNGWZ010000000
VERSION     DBNGWZ010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_335016"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16CX_17770"
     CDS             complement(<1..386)
                     /locus_tag="AC16CX_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR3014394.1"
                     /translation="MPLKTVSGKHADPRKGRGAGINPEGRFETVAREAFDDGWDRQEE
                     ELPPLKTHVTEERVKSIIARNDSPDIPFTQSINPYAGCEVGCVYCYARPTHAYRNLSP
                     GIDWETRLFAKVNAAEKLREELSRPG"
     gene            complement(443..1315)
                     /locus_tag="AC16CX_17775"
     CDS             complement(443..1315)
                     /locus_tag="AC16CX_17775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR3014395.1"
                     /translation="MRTLNIIGSGRVGRACGRLWARARVFEIQDVLTRSRASAAEGVK
                     FIGAGHAVGHLDEMRSADVWMVATRDDAIAPSCVTLAASGKITPDDIVFHVSGATPSG
                     DLKPVKDKGALIASVHPIKTFTDAEQAAQTFAGTFCSAEGDAEALRVLKPAFEKIGAR
                     VFDIAPELKPVYHSGGVFACNYLAALIEAALRAHEKAGIPRSASLKALEPMVRETVDA
                     IFDKGPAKALTGPISRGDVATVKRQLAMVESWDPQVGALYRGLGLLAVALAESDRRLD
                     ARRATELRETLSSK"
     gene            complement(1342..1719)
                     /locus_tag="AC16CX_17780"
     CDS             complement(1342..1719)
                     /locus_tag="AC16CX_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014396.1"
                     /translation="MLLRRADKEFAACVRQIPYREELKWAADMDLQEFRLSLSAPRPP
                     RGLAAPLRALWHAARGNWNRAHEIVAAQDGKAAARVHAYLHRKQGDSVNADYWYYRAG
                     AQRPHGSLDKEWRTLARQFLMRP"
     gene            1738..3276
                     /locus_tag="AC16CX_17785"
     CDS             1738..3276
                     /locus_tag="AC16CX_17785"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR3014397.1"
                     /translation="MPFSRRDFLHCAAGLAAAALTLPRLLAQPRFEGYPFTLGIASGY
                     PTPEGVVLWTRLAPEPLTGGGTPAAAVPVGWEVATDEAFRHVVRKGRETAVPRWAHSV
                     RAEVEGLDPAHDYFYRFHAGGAVSPVGRTRTAGAAAQRLRFAFASCQQYEQGYYVAYR
                     HMAAEDLDLVVHLGDYIYESSWGRNHVRKHGAGEPVTLGEYRDRYALYKTDPDLQAAH
                     AAFPWIVTWDDHEVQNDYANDRSQYLDEPAFFLERRAAAYQAYYEHMPLPKRMQPSGP
                     DMRIYTRADFGGLARFHVLDDRQYRDHQVCPRPGRGGSNVVPAAECPGLQRADRTLLG
                     AEQERWLHDSLRSSGARWNVIAQQTLMAQVDRTPGEGQSFWTDGWDGYPKAREKLLEF
                     IAGKRIANPVVIGGDVHMCVVADLKTDFDDPKTPVVATEFVGTSITSQGLAQKQLESW
                     RADNPHIHYANSTRRGYTTLELTAKRCVARLRTVNEKDPDSPVRTLAAWTVEDGRPGA
                     QRGA"
     gene            3461..4057
                     /locus_tag="AC16CX_17790"
     CDS             3461..4057
                     /locus_tag="AC16CX_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR3014398.1"
                     /translation="MFQIRLHGRGGQGVVTGAEMLSIAAFLQGRHAQAFPSFGSERTG
                     APVVAYCRIDDREIRLREPIVEPDALIVQDPTLLHQVDLFSGLKRPDGYILINTTRSF
                     EELGIGDFIAGFHAERLCTVPATELALKHVGRPVPNAGLLGGFAAASGLITLESVAAA
                     IRDRFSGKVADGNIAAATEAYHIVKREMEEATEHVEAD"
     gene            4041..5294
                     /locus_tag="AC16CX_17795"
     CDS             4041..5294
                     /locus_tag="AC16CX_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3014399.1"
                     /translation="MLKQIEGSHGVAEAVALCRPEVICAYPISPQTHIVEALGEMVKV
                     GALRPCEFINVESEFAAMSVAIGASAAGARAYTATASQGLLYMVEAMFNAAGLGLPVV
                     MTVANRAIGAPINIWNDHSDAMSQRDCGWIQLFAENNQEALDLHIQAFRLGEELSLPV
                     MVCMDGFILTHAYERVDLPTQAQVDAYLPPYEPRQVLDPREPVSIGAMVGPDAFAEVR
                     YLAHAKQMQALELIPQHATEFKEVFGRGSGGLIRPYRTEDAETIVVALGSALGTIKDT
                     VDEMRAEGHKIGVLGIISFRPFPLAAVRGVLRHAKRVVVLEKSLAVGIGGIVSANVRM
                     ALSGIHLHGYTVIAGLGGRPIVKKSLHKLFRQAERDELEHLIFLDLDLNIIHRELERE
                     KQKRRSGPIAENILRDVGVVAAKVV"
     gene            5305..>6260
                     /locus_tag="AC16CX_17800"
     CDS             5305..>6260
                     /locus_tag="AC16CX_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3014400.1"
                     /translation="MAFQPIKFYQTGTFTVGNRLLDQGERSVQANMQRTNSLNSGHRA
                     CQGCGEALGARYAIDAAMNATKNQLIAANATGCLEVFSTPYPETSWQIPWIHSLFGNT
                     AAVATGIAAALRVKGRHDVRVIAQGGDGGTTDIGFGCLSGLFERNDDVLFICYDNEAY
                     MNTGVQRSGATPGGAWTNTTAAGKKQEKKDLGGIMAAHRIPYFATVSPGYFEDMVAKM
                     RRAIATRGFRMLHCLSPCPPGWKMDERNSLKAARLAVETRVFPLYEVMGGVEWRITQA
                     PAPRSVEEYLSLQGRFGKLTPDRVEHIQREVADDYALLARRI"
BASE COUNT         1009 a         2192 c         2066 g          993 t
ORIGIN      
        1 tagccagggc gcgaaagctc ctcgcgcagc ttctccgcgg cgttcacctt ggcgaagagc
       61 cgcgtctccc aatctatgcc cggcgagaga ttgcggtagg cgtgcgtggg acgagcatag
      121 caataaacgc atcccacctc gcatccggcg taagggttga tcgactgcgt gaacgggatg
      181 tcgggcgagt cgttgcgcgc gatgatgctc ttcacccgct cctcggtgac gtgcgtcttg
      241 agcggcggca gttcttcttc ctgcctgtcc cacccgtcgt cgaacgcctc gcgcgcgaca
      301 gtctcgaagc ggccctcggg attgatgccc gcgccgcggc ccttgcgcgg gtcggcgtgc
      361 ttgccgctga cggttttcaa aggcatggaa tgtgtgatcc gtgatccgtg atccgtgatc
      421 cgacaaagtc tatgccggca ctctacttgg aggaaagcgt ctcgcggagc tcggtggcgc
      481 gccgcgcgtc gagccgccgg tcggactcag cgagcgcgac cgcaagcagc ccgaggccgc
      541 ggtagagcgc gccgacctgc gggtcccagc tctcgaccat ggcgagctgg cgcttcaccg
      601 tcgcgacgtc cccgcgcgag atcgggccgg tcaacgcttt tgccggcccc ttgtcgaaga
      661 tcgcgtccac ggtttcgcgc accatgggtt ccagcgcctt gagcgacgcc gagcgcggga
      721 ttcccgcttt ctcgtgagcg cgcagcgccg cctcgatcag cgccgccagg tagttgcacg
      781 cgaacacccc gcccgagtga tagaccggct tcagctccgg ggcgatgtcg aatactctcg
      841 cgccgatctt ctcgaacgcg ggcttgagca ccctcaacgc ctcggcgtcg ccctcggcgc
      901 tgcagaaggt gcccgcgaac gtctgcgccg cctgctcggc gtcggtgaag gtcttgatcg
      961 ggtgcacgct cgcgatgagc gcccccttgt ccttgaccgg cttgagatcg cccgagggcg
     1021 tcgccccgct cacgtgaaac acgatgtcat ccggggtgat cttgcccgag gcggcgagcg
     1081 tcacgcagct cggcgcgatc gcgtcatcgc gcgtggcgac catccacacg tcggcggagc
     1141 gcatttcgtc gagatggccg accgcgtggc ccgccccgat gaacttcacg ccctcggcgg
     1201 cgctcgcgcg cgagcgcgtc agcacgtcct ggatctcgaa cacgcgtgcc cgcgcccaca
     1261 gccggccgca cgcgcggccg acccgtccgg atcctatgat gttgagcgtc ctcacactcc
     1321 gccccggcta ttcgtcgctc gtcacggtct catcaggaat tggcgcgcca gcgtccgcca
     1381 ctccttgtcg agcgagccgt gcggccgctg cgcccccgcc ctgtagtacc agtagtcggc
     1441 gttcacgcta tcgccttgct tgcgatgcag ataagcatgc actcgtgcgg cggctttacc
     1501 gtcctgcgcc gccacgattt cgtgggcccg attccagttg cccctggccg cgtgccacaa
     1561 cgcgcgcagc ggggctgcga gtccgcgcgg cgggcgcggt gcgctgagtg acagccggaa
     1621 ttcctgcagg tccatgtccg cagcccattt taactcttcc cggtaaggaa tttgcctgac
     1681 gcaggccgca aattccttgt cagcgcgccg caacagcacc gcgttatagt gaccagcatg
     1741 ccgttctccc gacgtgattt tctgcactgc gccgccggcc tcgccgccgc ggcgctgacc
     1801 ctgccccgcc ttctcgcaca gccgcggttt gaaggctatc ctttcacgct cggcatcgcc
     1861 tcgggctacc ccacgccgga gggcgtcgtg ctgtggacgc ggctggcgcc cgagccgctc
     1921 actggcggcg gcacaccggc ggccgcggtc ccggtgggat gggaggtcgc gaccgacgag
     1981 gctttccgcc acgtcgtgcg caaggggaga gaaaccgccg tgccgcgctg ggcgcactcg
     2041 gtgcgcgcgg aggtggaggg tctcgatccc gcacacgact atttttaccg ttttcacgcc
     2101 ggcggcgcgg tgagccccgt cggccgcacc cgcacggcgg gcgccgcggc gcagcgcctg
     2161 cgattcgcat tcgcctcgtg ccagcaatac gagcagggat attacgtcgc ctaccgccac
     2221 atggcggccg aggatctcga cctcgtcgtc cacctcggcg actacatcta cgaatcgtcg
     2281 tggggccgca accacgtacg caagcacggg gccggggagc cggtgacgct cggcgagtac
     2341 cgcgaccgct acgcgctgta caagaccgac ccggacctgc aggccgccca tgcggccttc
     2401 ccgtggatcg tcacgtggga cgatcacgaa gtgcagaacg attacgccaa cgaccgctcg
     2461 cagtacctcg acgagccggc gttcttcctg gagcgccgcg ccgcggccta ccaggcctac
     2521 tacgagcaca tgccgctgcc gaagcgaatg cagccgagcg gccccgacat gcggatctac
     2581 acgcgggcgg atttcggagg gctcgcccgt tttcacgtgc tggacgaccg tcaataccgc
     2641 gatcaccagg tctgtccccg gcccggccgc ggcggcagca acgtcgtgcc ggcggccgaa
     2701 tgcccggggc tgcaacgcgc cgatcgcacg ctgctcggcg cggaacagga gcggtggctc
     2761 cacgacagcc tcaggagttc gggcgcgcgc tggaacgtga tcgcgcagca gactctgatg
     2821 gcgcaggtgg accgcacgcc gggcgaggga cagtcgttct ggaccgatgg ctgggacggc
     2881 tatccgaaag cgcgcgagaa gctgctcgaa tttatcgccg gcaagcgcat cgcgaacccg
     2941 gtggtgatcg gcggcgacgt gcacatgtgc gtcgtcgccg atctcaagac cgatttcgac
     3001 gatccgaaga cgccggtggt ggcgaccgag ttcgtcggca cctcgatcac ctcgcagggc
     3061 ctggcgcaga agcaactcga gagctggcgc gccgacaacc ctcacattca ctacgccaac
     3121 agcacgcgcc gcggctacac cacgctcgaa ctcacggcga agcgctgcgt cgcgcgtctg
     3181 cgcacggtga acgagaagga tcccgactcg ccggtgcgca cgctcgccgc ctggacggtg
     3241 gaggacgggc ggcccggagc gcagcgcggc gcctgatgtg cgaaaaaccg ggagatcggg
     3301 agggggagga tcgcaagaaa ctgactccac ctcacgcgct cccgccctcc cggatttttt
     3361 cgctctcccg ccctcacgcc ctcccggatc tgtttgatct agctcaaacc cctcccctcc
     3421 ccgcgggctt acattgtccg gcgaggagga cgaggggctc atgttccaga tacgcctgca
     3481 cgggcgcggc ggccagggtg tggtgaccgg cgcggaaatg ctctccatcg ctgcgttcct
     3541 gcagggccgg cacgcgcagg cattcccgag cttcggctcc gagcgcaccg gggcgccggt
     3601 ggtggcgtac tgccgcatcg acgacaggga gatccggctg cgcgagccca tcgtcgagcc
     3661 ggacgcgctc atcgtccagg acccgacgct gctccaccag gtggacctct tcagcgggct
     3721 caagcgcccg gacggctaca ttctcatcaa cacgacgcgc agcttcgagg agctcggcat
     3781 cggcgatttc atcgcgggct tccacgccga gcggctgtgc acggtgcccg ccaccgagct
     3841 cgcgctcaag cacgtcgggc gcccggtgcc gaacgcgggg ctgctgggcg gctttgcggc
     3901 ggcgagcggg ctcatcacgc tcgagtcggt cgctgccgcg atccgcgacc ggttctcagg
     3961 caaggtagcc gacggcaata tcgccgccgc caccgaggcc tatcacatcg tcaagcggga
     4021 aatggaggag gcgacagagc atgttgaagc agattgaagg ttcccacggc gtcgccgagg
     4081 cggtcgccct gtgccgcccc gaggtgatct gcgcctaccc catctcgccg cagacccaca
     4141 tcgtcgaggc cctcggcgaa atggtcaagg tgggcgcgtt gcggccgtgc gagttcatca
     4201 acgtcgagtc ggagttcgcc gcgatgagcg tcgcgatcgg ggcttccgcc gcgggcgcgc
     4261 gcgcctatac cgccaccgcg agccagggcc tgctctacat ggtcgaggcg atgttcaacg
     4321 ccgccggtct cgggctgccc gtcgtcatga cggtggcgaa ccgcgcgatc ggcgcgccga
     4381 tcaacatctg gaacgaccac agcgacgcca tgtcgcagcg cgactgcggc tggatccagc
     4441 tcttcgccga gaacaaccag gaagcgctcg acctgcacat ccaggcgttc cgcctgggcg
     4501 aggagctttc gctgcccgtc atggtgtgca tggacggctt catcctcacg cacgcctacg
     4561 agcgcgtgga cctgccgaca caggcgcagg tggacgccta cctgccgccg tacgagccgc
     4621 gccaggtgct cgatccgcgg gagccggtgt cgatcggcgc catggtgggg ccggacgcct
     4681 tcgccgaggt gcgttatctc gcgcatgcca agcagatgca ggcgctcgag ctcatcccgc
     4741 agcacgcgac ggagttcaag gaagtgttcg gccgtggctc gggagggctc atacgcccgt
     4801 accgcaccga ggacgccgag accatcgtgg tcgcgctggg atccgcgctc ggaaccatca
     4861 aggacaccgt ggacgaaatg cgcgcggaag gacacaagat cggggtgctc ggcatcattt
     4921 ccttccggcc cttcccgctc gccgccgtcc gcggcgtcct caggcacgcg aagcgggtgg
     4981 tggtcctcga gaagagcctc gccgtgggca tcggcggaat cgtgtcggcg aacgtgcgca
     5041 tggcgctctc cggcatccac ctgcacggct acacggtgat cgccggcctc ggcggccgcc
     5101 ccatcgtcaa gaaatcgctg cacaagctgt tccgccaggc ggagcgcgac gagctcgagc
     5161 acctcatctt cctcgacctc gacttgaaca tcattcaccg cgagctcgag cgcgagaagc
     5221 agaagcgccg ctcggggccg atcgcggaga acatcctgcg cgacgtgggc gtggtcgcgg
     5281 cgaaggtcgt gtgaaggagg cgccatggca tttcagccca tcaagttcta ccagaccggc
     5341 accttcaccg tcggcaaccg cctgctcgat cagggcgagc gctcggtgca ggccaacatg
     5401 cagcgcacca actcgctcaa ctccgggcac cgcgcgtgcc aaggctgcgg cgaggcgctc
     5461 ggcgcgcgct acgccattga cgccgcgatg aacgcgacga agaaccagct gatcgcggcc
     5521 aacgcgacgg gctgcctgga ggtattttcc acgccgtatc ccgagacctc gtggcagatt
     5581 ccgtggatcc acagcctgtt cggcaacacc gctgcggtcg ccaccggcat cgccgccgcg
     5641 ctccgggtga agggccgcca cgacgtgcgc gtgatcgcgc agggcggcga cggcggaacc
     5701 accgacatcg gatttggatg tttgtcgggc ctattcgagc gcaacgacga cgtgctcttc
     5761 atctgctacg acaacgaggc ctacatgaac accggcgtgc agcgctccgg cgccacgccg
     5821 ggcggggcct ggaccaacac caccgccgcc ggcaagaagc aggagaagaa ggacctgggc
     5881 ggcatcatgg ccgctcaccg catcccttac ttcgccaccg tctcgccggg ctacttcgag
     5941 gacatggtgg ccaagatgcg gagggccatc gccacccgcg gcttccgcat gctccactgc
     6001 ctctccccct gcccgccggg ttggaagatg gacgagcgga acagcctcaa ggcagcgcgg
     6061 ctggcggtgg agacgcgggt cttcccgctc tacgaggtca tggggggagt ggagtggcgc
     6121 atcacccagg ccccggcgcc gcggtcggtc gaggagtacc tctcgctgca ggggcgcttc
     6181 ggcaagctga ccccggatcg ggtggagcac atccaacgcg aggtggccga cgactacgcg
     6241 ctcctggccc gccgcatctt
//
LOCUS       DBNGWZ010000569         8123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2726440, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000569 DBNGWZ010000000
VERSION     DBNGWZ010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2726440"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16CX_17805"
     CDS             complement(<1..205)
                     /locus_tag="AC16CX_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014401.1"
                     /translation="MVASITVVFAVLCTAFVWQRGLASFADDSVSYLVMAQVFSPWQP
                     ASPPVAEAFAREAFYPPLFPVLLA"
     gene            complement(238..1056)
                     /locus_tag="AC16CX_17810"
     CDS             complement(238..1056)
                     /locus_tag="AC16CX_17810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR3014402.1"
                     /translation="MPQLPPLKIVDVGAMYLGEDTNPYDPMLQSLSCEVVGFEPVAAE
                     YEKLNTHYAGRFTYLPHVVGDGSSQTFYECAAAYNSSLFEPNMALIARFSGLEEFFRV
                     VATRSVVTKRLDDIPEASGADFLKLDVQGGELMVLEGAIRTLRDVLVVHTEAVFLPLY
                     KNQPLFSDIDPWLRARGFVLHKLYHFGGQLFKPFPFVDRPDTGISQVMWCDAVYVRDF
                     LELKRLSEAQLLKLAAILHANYGSHDFAAVALGAFDRRAQSALQRRYIERLAAE"
     gene            complement(1239..3071)
                     /locus_tag="AC16CX_17815"
     CDS             complement(1239..3071)
                     /locus_tag="AC16CX_17815"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase,
                     SPINDLY family protein"
                     /protein_id="HIR3014403.1"
                     /translation="MCRSASTSSRISDDGVDAEPLMNTSDPAGYWFRRGVERLDAGLF
                     EDAESCYRKALEIEPRHAKAKVNLGMILQRRGEEVEAARCYRDALAVDPGIAQAWFNL
                     GTMLLDREEPASALDHFREAVRLDPGRAVWHSALGSACQRAGRPLDALASLQTALRLD
                     PKLTSAHSDLGVCLLNMGDAASAAASFRQAQELDPSMQDASSNLLFALNFIPGLDPDA
                     VYREHVAWGQRFDELAQQSAHGNDPDPERRLRIGYLSPDFREHALSFFIEPVLEQHDR
                     SRFEAVCYSDAEREDAVSRRLRGLSILWRASAGWNDEQLAEQIRQDGIDILVDLAGHS
                     AGGQRMLLFAGKPAPVQVSWLGYLNTTGLEAMNYRITDWHACPREMERYHTERLIRMP
                     VSQWCYRPPAGAPEVGPLPAAHEGTITFGSFHNLAKITPQVIALWAAILRDIAGSRLI
                     VVAHGSEQLTGRLGDRFRAHGVDPGRIEGRGGMPLNAYLSLHNRVDISLDAFPYSGGT
                     TTFHSLWMGVPIVTLSGQNVVSRGGASILSALDLRELIARNEHHYVNIAVELASDRER
                     LGRLRRELRQRLARSPLTDAARFTAALETAYREAWRLWCVRQSG"
     gene            complement(3034..7374)
                     /locus_tag="AC16CX_17820"
     CDS             complement(3034..7374)
                     /locus_tag="AC16CX_17820"
                     /inference="COORDINATES: protein motif:HMM:NF025214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-linked N-acetylglucosamine transferase family
                     protein"
                     /protein_id="HIR3014404.1"
                     /translation="MLTSLLAKVMRRRPDAPRAPERETLDAWLREGYDHQRRGELVEA
                     QRIYSRILDYDPRDPDALFFLGLVAMNDGREARAADLFERAIDARPNDPAFWFSLGVI
                     CYRLRRLQQAATACRAGIALQPENADMRNNLAAVLVESGRTEEAREIIEQLVASGLGL
                     GSAYFNLGGIYRDYARIEEAIAVCRKAVELNPDNPDAYTNLLLTLNYSPDCDAATLLQ
                     EHRRFGARFARPYVEPQPDRTWPRRLRVGYLSPDFRKHVVAAFMEPVLANHDRERFEV
                     FCYYNQRHEDEYTQRFRQLADHWLDCVHISDNELAERIRGDGIDILVDLAGHTNGQRL
                     LVFAARPAPVQVTYLGYPNTTGLAAIDYRITDARADPPGESDRLSAEKLMRLPDCFHC
                     YRPREDSPAVGPLPAAASGRVTFGCFNNFPKASDPFLDAAARILAMVPGSRLKLKSKA
                     LAVPHVAQRVRERFVRAGIDPARVELRGWETSVRSHLAAYGSIDIAIDTFPYNGTTTT
                     CEALWMGVPVVTLVGDRHAGRVGSSLLNAVGLGELVAKDAEGYVATSVALAGNLGSLG
                     ELRSGLRQRMRQSPLMDEAGFTRALERCYVEIWENKIKALPGARSSGDEAIVESLRRA
                     AELRAAGRTSEAEAAYQAVLLEQPDQIDALTALWDFSHETGNHGAAVDWLRRGIAANG
                     GAPMLHYMMGCSLLAQGNPGDAAASFRAALALDPSMARAHNNLGCALEALGRPMEARE
                     CYRRAIDLDPKLADALYNLGNVCRQLGDITHAVEYIRQALEREPGRADWHCNIGDLLY
                     HRLQLDEAVQSYARAVAVDREHARAYSGRALAYQALGFADEAETDFRMAMQLQPDNAE
                     MHSNWLLSLHYRRGEETQALFDAHFAWAKRHASAISRQAARDGHERYPRRRLNIGYMS
                     PDFRRHSVASFIEPVLAAHDRKRFKVFCYSNVAYPDELTQRLRGLCEEWRDISATSDP
                     WVAQQIRADRIDILVDLAGHTAGGRMLLFAGKPAPVQVTWLGYPNTTGLAAIDYRLTD
                     AIADPEGQTERFHSETLVRLAGGFLCYRAPSESPQVGELPLLQAGYVTFGSFNNLARV
                     TPAMIALWAQLLRALPDARLVLKASGLAAKSARQALLGQFSDLGIPGERLETCGQEES
                     FALHLAKYNEIDIALDAFPYSDAMTTCEALWMGVPVVTLAGTTHVSRVGASILTRVGL
                     ADLVAETGDDYVRKALQLARDVERLRALRSGMRERMRSSPLLDVGGFTAALEQAYAAV
                     WDRWCESEEQSDRVLRLHVGGQQVMPGWKILNVQPGPGVDFVGDCSDLGQFADASVDE
                     IYASHVLEHLGYAEKLPRALAEFCRVLKKGGAARISVPDFEVLCRLFLDPLQTADDRM
                     LIMRMAFGGQTDPHDFHHVGLTFEFLGAYLSNAGFAKIERVRDFGMFEDASLTRVSGV
                     PISLNVIAYK"
     gene            complement(7646..8092)
                     /locus_tag="AC16CX_17825"
     CDS             complement(7646..8092)
                     /locus_tag="AC16CX_17825"
                     /inference="COORDINATES: protein motif:HMM:NF012342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR3014405.1"
                     /translation="MRYLQKGFTLIELMIVVAIIGILAAVAIPAYQDYIARSQVSEAV
                     SLMGSGKTPFAEYFADKGVWPPTTADVMGNTSGKYTASIAITGTPVTVAPGAITLMAT
                     MQPSGVNANITGGTVLLETADGGKIWTCKVGGTLPLNPKYVPGACR"
BASE COUNT         1538 a         2632 c         2481 g         1472 t
ORIGIN      
        1 ccgccagcaa caccggaaat agcggcgggt aaaatgcctc gcgcgcgaac gcctcggcca
       61 caggtggcga tgccggctgc cacggcgaaa aaacctgcgc catcacgaga tagctaacgc
      121 tgtcgtcggc aaacgaagcg agtccgcgct gccagacgaa agccgtgcaa agcaccgcaa
      181 ataccaccgt gatcgatgcg accatcaggt ccggcagcga ggagcgtaga tcgaatctca
      241 ttcggcggca aggcgttcga tataccgacg ctgcagggcc gattgcgcgc ggcggtcgaa
      301 cgctcccaag gcgaccgcgg caaaatcgtg cgatccgtaa ttcgcatgaa gaatcgccgc
      361 tagcttgagc agttgcgcct cggataggcg ctttagttcc aggaaatcgc gcacgtaaac
      421 cgcatcacac cacatcacct ggcttattcc ggtgtccggc cggtccacga atgggaatgg
      481 tttgaacagc tgcccgccga aatggtacag cttgtgcagg acaaaacctc gggcgcgcag
      541 ccaaggatca atgtcggaaa acaaaggctg attcttgtac agggggagga acacggcctc
      601 cgtgtgaacg actaggacgt cgcgcagcgt ccggatcgca ccttcgagga ccatcaattc
      661 gccgccttgg acatcgagct tgagaaaatc cgcgcccgac gcctcgggaa tgtcgtcgag
      721 tcgcttggtg acaacgctgc gcgtcgcaac caccctgaag aactcctcga gcccgctgaa
      781 tcttgcaatc agcgccatat tcggctcgaa cagcgacgag ttgtacgcgg cggcgcactc
      841 gtaaaatgtc tgcgaggagc cgtctccaac gacgtgcggc agataggtaa atcggcccgc
      901 gtagtgcgta ttgagcttct cgtattcagc cgccaccggc tcgaatccta caacttcgca
      961 ggacagcgac tgtagcatcg gatcataagg attcgtatcc tctcccaggt acatcgcgcc
     1021 gacgtcgacg atcttgagcg gcggcagctg gggcacgatg gattcgatcg ggaacgcgga
     1081 cacgatgact gacgatcctt agtcgcgggc tgagccgcaa cagaattccg ggttcaggtt
     1141 ctcggccaag cgcgcacgcc acagaggcgc tggctgcgat gccgcctgca ggccagcggt
     1201 catgcggacg agttgccagc gccggtccat ctcgggcatc agccagattg acgaacacac
     1261 cataatcgcc atgcctccct gtacgccgtt tccaaggcgg cggtgaagcg cgcagcgtca
     1321 gtaaggggcg atcgagccag gcgctggcgc agctcgcgac gcaaacgccc gagccgctcc
     1381 cgatcgctcg cgagttccac ggcgatattc acatagtggt gctcgttccg tgcgataagc
     1441 tcgcgcaaat ccagcgcgct caagatgctt gccccgcccc ttgacacgac gttctggccg
     1501 ctgagagtaa cgatcgggac gcccatccac agcgaatgaa acgtcgtggt tccgcccgag
     1561 tacggaaacg cgtccagact gatatcgaca cggttgtgca gcgacagata ggcgttgagc
     1621 ggcatcccac ctcgcccctc gatccgcccc gggtccacgc cgtgagcgcg gaaccggtcg
     1681 ccaaggcgtc cggtgagttg ctccgacccg tgcgccacta cgatgagcct ggaaccggct
     1741 atgtcgcgca gtatcgcggc ccatagcgcg atcacttgcg gcgtaatttt cgccaggttg
     1801 tgaaacgatc cgaacgtgat ggtcccctca tgcgccgcgg gcagcggccc cacttccggc
     1861 gcaccggcag gcggcctgta acaccactgg ctgaccggca tgcggatcaa ccgctccgta
     1921 tggtagcgct ccatctctct cgggcaggca tgccaatcgg tgatccggta attcatcgcc
     1981 tcgagcccgg tcgtgttcag gtagccgagc caggaaacct gaaccggcgc gggctttccg
     2041 gcgaaaagca gcatgcgttg ccctccggca ctgtgcccgg ccaggtcgac cagtatgtca
     2101 atcccgtcct gccgtatttg ctcggcgagt tgttcatcat tccatccggc cgaagcacgc
     2161 cacaggatgc ttaggccacg aagccgccgg ctgaccgcat cttcccgttc cgcgtccgaa
     2221 tagcacaccg cctcgaaccg cgaccggtcg tgctgctcga ggaccggctc gatgaagaag
     2281 gacagcgcgt gctcgcggaa gtcgggcgaa aggtagccga ttctcaacct tcgctccgga
     2341 tccggatcgt tgccgtgtgc actctgctgc gccagttcat cgaaccgctg tccccacgca
     2401 acgtgttccc gatacacggc gtcgggatcc agtccgggaa tgaaattgag cgcgaacagc
     2461 aggttgctcg acgcgtcttg catcgacgga tccagctcct gcgcctgtct gaacgaggct
     2521 gccgcagatg cggcgtctcc catgttcagc aggcacacgc ccaagtcgct gtgcgcgctc
     2581 gtcagtttcg gatcgagccg taaggcggtc tgcaggctgg ccaaggcatc gagcggccgt
     2641 cccgcgcgct ggcaggccga tccgagggcc gagtgccata cggcgcgtcc cggatcgagg
     2701 cgcaccgctt cgcgaaagtg atcgagcgcg gaagccggct cttcgcggtc gagcagcatc
     2761 gtgccgagat tgaaccaggc ctgcgcgatg cccggatcca ccgccagggc gtcgcgatag
     2821 cagcgggcgg cttcaacctc ttccccgcgg cgctggagga tcatcccgag attgactttt
     2881 gccttcgcgt gccgcggctc gatctccagc gccttgcgat agcagctttc cgcgtcttcg
     2941 aacaaacccg catccaggcg ttcgacgccg cggcggaacc aatagcctgc aggatcagag
     3001 gtattcatca agggttctgc atcaactcca tcgtcactta tacgcgatga cgttgaggct
     3061 gatcggcaca ccggagaccc tagtcaggct cgcgtcctca aacatgccga aatcccttac
     3121 ccgctcgatc ttcgcgaagc ccgcgttcga cagatacgcg ccgagaaact cgaaagtcag
     3181 gccgacatga tgaaaatcgt ggggatcggt ctgtcctccg aaagccatcc gcatgatcaa
     3241 catgcggtca tccgctgtct ggagcggatc gaggaacagc cggcacagga cttcgaagtc
     3301 ggggacgctg atcctggccg cgcccccctt cttgagcacg cggcagaact cggccagggc
     3361 gcgaggcagc ttctcggcgt agcccaggtg ctcgagcacg tgggaggcgt agatttcgtc
     3421 cacggacgcg tccgcaaact ggcccagatc ggagcagtcg cccacgaagt caacgcccgg
     3481 ccccggctgc acgttgagga tcttccatcc cggcatcacc tgttgcccgc cgacgtggag
     3541 ccgcagaacc cggtccgact gttcttcact ctcgcaccat cggtcccaca ctgccgcata
     3601 ggcctgctcc agcgcggcag tgaatccgcc cacgtcaagc agcggggaag accgcatgcg
     3661 ctcgcgcatg ccggaccgca gcgcgcgcaa tctttcgaca tcgcgcgcga gttgcagtgc
     3721 cttccgcacg tagtcatcgc ctgtttccgc gacaaggtcc gcgaggccga cccgggtgag
     3781 tatgcttgcg ccgaccctcg acacatgggt cgtcccggcc agtgtcacga cggggacacc
     3841 catccagagc gcctcgcatg tcgtcattgc atcgctgtac gggaaagcat cgagcgcaat
     3901 gtcgatttcg ttgtactttg cgagatgcag ggcgaaggat tcctcctggc cgcatgtttc
     3961 aagtctttcg cctgggatac cgagatccga gaactggcca agaagtgctt gtcgcgcact
     4021 tttcgcagca agaccactcg ccttcaggac cagccgcgca tccggcagtg cccgaagcaa
     4081 ctgcgcccag agcgcgatca tcgcgggagt caccctggcc aggttgttga aactcccgaa
     4141 agtgacgtat cccgcctgga gtagcggcaa ttcgcccact tgtgggctct ccgaaggggc
     4201 gcgatagcac aggaatccgc cagccaggcg cacaagcgtc tcagaatgga aacgctcggt
     4261 ttgtccttcg ggatcggcga tggcgtcggt cagacgatag tcgatggcag ccaatccagt
     4321 cgtgttggga tagcccagcc aggtcacctg aacgggcgcc ggttttccag cgaacaggag
     4381 catccgtccc cctgccgtgt ggccggcgag atccaccagg atatcgatgc ggtcggcgcg
     4441 gatctgctgc gctacccagg gatcggacgt ggcggaaatg tcacgccatt cctcgcacag
     4501 gccacggagg cgttgcgtga gttcatccgg gtaggcgacg tttgagtaac agaagacctt
     4561 gaatctcttg cgatcgtgcg ccgcaaggac cggctcgatg aaggacgcca ccgaatggcg
     4621 cctgaaatcc ggggacatat aaccgatgtt gagcctgcgc ctcgggtagc gctcgtgccc
     4681 gtcgcgagcc gcctggcggc tgatcgcgct tgcgtgccgc ttcgcccatg cgaaatgggc
     4741 gtcaaagagc gcctgggtct cctcgcctcg cctgtaatgc agcgaaagaa gccagttgct
     4801 gtgcatttcg gcgttgtcgg gctgcagctg catcgccatt cggaaatccg tctcggcctc
     4861 gtctgcgaat cccagtgcct ggtacgccaa cgcccggccc gaataggcgc gggcatgctc
     4921 gcgatcgacg gccaccgccc ttgcatagct ttggacagct tcatcgagtt gcagccggtg
     4981 gtacagcagg tcgccgatat tgcaatgcca atcggcccgt cccggttccc gttcgagcgc
     5041 ctgtcgtatg tattcgacgg cgtgcgttat gtctccgagc tgccggcaga cgttgccgag
     5101 gttgtagagc gcatcggcga gctttggatc gaggtcgatc gccctgcgat agcactcccg
     5161 ggcttccatc ggccggccca gcgcttccag cgcgcagccg aggttgttgt gggctcttgc
     5221 catcgaaggg tccagggcca atgccgcgcg gaatgaggcg gcggcatcgc cggggttccc
     5281 ctgcgccagg agcgagcaac ccatcatgta atgaagcatc ggcgcaccgc cgttcgccgc
     5341 gattccccgt ctcaaccaat cgaccgcggc gccatgattg ccggtctcgt gcgagaagtc
     5401 ccacagcgcc gtcagggcat cgatctgatc cggttgctcc agcaggaccg cttggtaagc
     5461 tgcttccgct tcgctcgttc ttccggcggc gcgaagctcc gcggcccggc gcagcgattc
     5521 cacgatcgcc tcgtcgcccg atgatcgggc ccccgggagc gccttgatct tgttctccca
     5581 tatttcgacg taacaacgtt ccagagccct ggtgaatccg gcttcgtcca tgagcggcga
     5641 ttgacgcatg cgctgacgca agccactgcg cagttcgccc aggctgccca agttgcctgc
     5701 cagggcgacg gacgtcgcca cataaccctc ggcgtctttc gccaccagct ctcccagccc
     5761 cacggcgttc agcaggctcg atcccacgcg tcccgcgtgg cggtcgccca ccagcgtcac
     5821 caccggcacg cccatccata gcgcttcgca tgtggtcgtg gtcccgttgt agggaaaagt
     5881 gtcgatcgcg atgtcaatcg agccgtacgc cgcaaggtgg cttcgcacgg acgtttccca
     5941 accgcgcagc tccacccggg caggatcgat tccggcccgg acgaagcgct cgcgcacgcg
     6001 ctgcgccacg tgcggaacgg cgagcgcctt gcttttcagc ttgaggcgcg aaccgggaac
     6061 catcgcgagt atccgcgccg ccgcgtcgag aaaggggtcc gacgccttgg gaaaattgtt
     6121 gaagcagcca aacgtaacgc ggcctgatgc ggcagccggc agcggaccga cggccgggct
     6181 gtcctcgcgc ggccgatagc agtgaaaaca gtccggcagg cgcatgagct tttccgcgct
     6241 caggcgatcg gactctcctg gcggatcggc gcgggcatcg gtgatgcggt aatcgattgc
     6301 ggccagcccg gtcgtgttcg ggtaaccgag atacgtcacc tgcaccggcg cgggcctagc
     6361 ggcgaaaacc agcagccgct gcccattcgt gtgccccgcc agatcgacga gtatgtcgat
     6421 gccgtcgccg cggatgcgct ccgccagttc gttgtcggag atatggacac agtcgagcca
     6481 atgatcggcc aactggcgga aacgttgggt gtactcgtcc tcgtgccgct ggttgtaata
     6541 gcagaaaacc tcaaagcgtt cacggtcgtg attcgccaga accggttcca tgaacgccgc
     6601 gaccacatgc ttgcgaaaat caggggatag gtatcccacg cgcaaacggc gtggccacgt
     6661 tcggtcgggc tgcggttcga cgtaaggtcg cgcgaaacgg gccccgaagc gccggtgctc
     6721 ctgcagaagc gtggccgcat cgcaatccgg gctgtaatta agggtcaaca gcaagttggt
     6781 gtaggcatcc gggttgtcgg gattgagctc cacggccttg cgacaaacgg cgatcgcctc
     6841 ctcgatcctg gcgtaatcgc gataaattcc tcccaggttg aaataggctg agccgagtcc
     6901 aaggccggag gcgaccagtt gctcgattat ctcgcgggct tcttcggtac ggccggattc
     6961 gactagcacg gcggcgaggt tgtttcgcat gtcggcgttc tcgggctgca acgcgatgcc
     7021 ggcgcgacaa gctgtagcgg cctgctgcag gcgcctgagc cggtaacaga tgaccccgag
     7081 cgaaaaccag aacgcgggat cgttggggcg ggcgtcaatc gccctctcga acaagtcggc
     7141 cgcccgcgcc tcccgcccgt cattcattgc gaccagcccc agaaaaaaca gggcgtcggg
     7201 gtcccgcgga tcgtaatcca gaatcctgct gtagatgcgc tgcgcctcga ccagttctcc
     7261 acgcctttga tgatcgtatc cctcgcgcag ccaggcatcg agcgtctcgc gctccggcgc
     7321 gcgcggcgcg tcgggcctgc ggcgcatgac cttggccaac aagctcgtta gcatgagctt
     7381 gcgcctgcag acgttgagga ctccggatgc ggggaaatca cgttcgaccg gcggcgttag
     7441 cgcgagatcg gcaattttgc cattgctccg gcatcgggtc caaagccgct gtccgagccg
     7501 ttcgcggctt cttggcgcgg caggaaaatg acagcataac tcggcagccg cggaccggcg
     7561 ccgcccccag ccaaaaaaaa ggcccgcggc gtggcgggcc tcgattcgct aatggatatt
     7621 attctgtggc tattcgctct ccagcttaac ggcacgcgcc cggcacgtac ttggggttga
     7681 gcgggagagt gccgcccacc ttacaagtcc agattttgcc gccgtcggcc gtctcgagca
     7741 gtaccgttcc gccggtgata ttggcgttga cgccgcttgg ctgcatcgtc gccatcagcg
     7801 taatggcgcc tggcgcgacc gtcacgggtg ttccagtgat cgctatcgac gctgtgtact
     7861 tacccgaagt attgcccatg acatcggccg tggtcggtgg ccataccccc ttgtccgcga
     7921 agtattccgc gaacggcgtc ttgcccgaac ccatcaggct caccgcctcc gacacctgcg
     7981 aacgcgcgat atagtcctgg taggccggga tcgccaccgc cgccagaata ccgatgatcg
     8041 cgaccacgat catcagttcg atcagcgtaa agcccttttg gagataacgc attgagtgcc
     8101 cctttcgtgg ttgaccattc aaa
//
LOCUS       DBNGWZ010000570         3416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1469303, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000570 DBNGWZ010000000
VERSION     DBNGWZ010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1469303"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="AC16CX_17830"
     CDS             <1..816
                     /locus_tag="AC16CX_17830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014406.1"
                     /translation="HIWAPDSPERPWPKNRTVKAQRDVPLGKDELLREMDAAGVNRVV
                     IVPPSWEGDRNDLGLAAAKAHPDRFAVMGRIDPEAPDSRGKIAGWRKQPGMLGLRFVF
                     HRSPLLELLTEGKADWLWPEAEKHGVPVYMLVPHRHMHLVDRIAGRHPRLKLVMDHSG
                     LVDGKDEEAFREFDKLLALAKHANVATKVSCFPFFTTDKYPFKKLHSHIRRAFDAFGP
                     KRTFWGTDLSRLPCTYRQGVTLFTEELPWLKGQDLEWVMGRGVCEWLDWKTQG"
     gene            948..1775
                     /locus_tag="AC16CX_17835"
     CDS             948..1775
                     /locus_tag="AC16CX_17835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014407.1"
                     /translation="MLVVDSQVHIWAANTPERPWPARHEPHKPEPITAEGLLREMNAA
                     GVNRCVLVPPSWEGERNDVCLAAAQKHPERFAVMGRLDPEAPESRGKIATWRKQPGML
                     GLRFTVHRPFLRPLLTEGRLDWLWPEAEKAGVPIMTLALHTDMHLIDKVAERYPGLRL
                     TIDHLGLTTGKDEQAFRHLDNVTALAKRPNVAVKVSCLPYYTTDKYPFHKLHQYIRRV
                     YDAFGPKRMFWGSDLSRLPCSYRQGVTYFTEEIPWLGAGDKEWIMGRGVCEWLGWKG"
     gene            1888..2181
                     /locus_tag="AC16CX_17840"
     CDS             1888..2181
                     /locus_tag="AC16CX_17840"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3014408.1"
                     /translation="MTKLEFYDPSGAIEVTKPHARRLASLEGKRIGFVSNEQWQAYRM
                     LPMLKEMLEADFPGITVLPIDTFPQGNALIGTEETAALVKKSGVEAVIVGNAA"
     gene            2278..>3416
                     /locus_tag="AC16CX_17845"
     CDS             2278..>3416
                     /locus_tag="AC16CX_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014409.1"
                     /translation="MKNAVAGVGFAPDAAMVTFPIQMFLPGSDLAVLKERKREFYDGL
                     TRWKSEFAQSTAGAGKMIAVEGATYEDALVRANNLLLANQWGDGLSLWPATKERVDWV
                     LRGSALPRTHVLGKFPPRGGITTIGTCAIALAMAGGRPEYLPVLVAAVEACLESEGFD
                     QLQATSGGPFPAVIVNGPIAKQIRLNSGFGLLGPDPQRPAGASIGRALRLLQQNVGGA
                     LPGIGTMAIFGAMRYTNAVFAEDEDGLPPGWQPHATQRHGFAPGTNSVSVVFASGVTN
                     VRRRGAKKETPEEDALNGMWRMADFMRTPNLAVLHGWEKGTPGIMMFAPVVARRMAEL
                     GWTQRSIREFLWENSKIPMEQMRRAGAPAWIEIDANPVTRASINL"
BASE COUNT          608 a         1084 c         1172 g          552 t
ORIGIN      
        1 catatttggg cgcctgattc gccggagcgc ccgtggccga aaaaccgtac ggtcaaggcg
       61 cagcgcgacg tgccgctcgg caaggacgag ctgctgcgcg aaatggacgc cgccggcgtc
      121 aatcgcgtcg tgatcgtgcc gccgtcatgg gaaggggatc gcaatgatct cgggctggcg
      181 gcggcaaagg cgcacccgga ccgctttgcc gtcatggggc gcatcgatcc ggaagcgccg
      241 gattcgcgcg gcaagatcgc gggctggcgc aagcagccgg gcatgctcgg gctgcgcttc
      301 gtcttccacc gctcgccgct gctcgagctg ctcaccgagg gcaaggcgga ctggctgtgg
      361 cccgaggcgg agaagcacgg cgtgccggtg tacatgctgg ttccccaccg gcatatgcat
      421 ctcgtcgacc ggattgccgg acgccatccc cggctgaaac tggtgatgga ccactctggt
      481 ctggtggacg gcaaggacga agaggcgttc agggaattcg acaagctgct cgcgctcgcg
      541 aaacacgcga atgtcgcgac caaggtgagc tgcttcccgt tcttcaccac tgacaaatat
      601 cccttcaaga agctccactc ccacatccgc cgcgccttcg atgcattcgg ccccaagcgc
      661 acgttctggg gcaccgatct ctcgcgcctg ccgtgcacct accggcaggg cgtgacgctg
      721 ttcaccgagg aactgccctg gctcaagggg caggacctgg agtgggtgat ggggcggggg
      781 gtgtgcgagt ggctcgactg gaagacccaa ggatgaggga tgagggatga gggatgaagg
      841 atgacacccg cagcgcggga gccagaacca ggtcatcctt ccgccttcat ccttcgcgcc
      901 caaatgcttc cagatgcgat gaacgctgac aaccaagggc ttttgccatg ctagttgtgg
      961 attcccaagt acatatttgg gcggcgaata cgccggagcg gccgtggccg gcgcggcacg
     1021 agccgcacaa gccggagccg atcactgcgg agggcttgct gcgcgagatg aacgccgccg
     1081 gcgtgaaccg ctgcgtgctg gtgccgcctt catgggaggg cgagcgcaac gacgtctgcc
     1141 tagccgccgc gcagaaacat ccggagcgct tcgccgtcat ggggcggctc gatccggaag
     1201 cgccggagtc gcgcgggaag atcgcgacct ggcgcaagca gcccggcatg ctgggcctgc
     1261 gtttcaccgt gcaccggccg ttcctgcggc cgttgctgac cgaggggcgc ctggactggc
     1321 tgtggccgga ggcggagaaa gcgggcgtgc cgatcatgac gctcgcgctc cacacggaca
     1381 tgcacctcat cgacaaggtc gcggaacgct acccggggct caggctcacg atcgaccacc
     1441 ttggcctcac caccggcaag gatgagcagg cgttccgcca tctcgacaac gtcaccgcgc
     1501 tcgcgaaacg ccccaacgtc gcggtgaagg tgagctgcct gccgtattac acgacggaca
     1561 agtatccctt tcacaagctg caccagtaca tccgccgtgt ttacgacgcg ttcgggccga
     1621 agcgcatgtt ctggggtagc gacctctcgc ggttgccgtg cagctaccgg cagggcgtga
     1681 cgtacttcac cgaggaaatc ccctggctcg gcgccggcga caaggagtgg atcatggggc
     1741 gcggggtgtg cgagtggctc ggctggaaag gctagattcg tgataggtga caagtgatag
     1801 gtgataggtt gtaggaactt actcccgcgc gagcgcgggt aacttatcac ttatcacgct
     1861 tttcatgatt tgatgagagg agtgcagatg accaaactgg aattctacga tccctccggt
     1921 gcgattgaag tgacgaagcc gcatgcgcgg cggctcgctt cgctcgaggg caagcgcatc
     1981 ggcttcgtca gcaacgagca gtggcaggcc taccgcatgt tgccgatgct gaaggaaatg
     2041 ctcgaggccg actttccggg catcacggtg ctgcccatag acacttttcc acagggcaac
     2101 gccctgatcg gcaccgagga gaccgctgcg ctggtgaaga agagcggcgt cgaggcggtc
     2161 atcgtcggca acgcggcttg aggggcctgc gccacagcat gcggccgtgc agccgctagg
     2221 atagaagcgc tgggcatccc gaccgtcacg ctgacgcggg cggatttcgt cggggtcatg
     2281 aagaacgcag tcgcgggcgt cggtttcgcc ccggacgctg cgatggtcac gttcccgatc
     2341 cagatgttcc tgcccgggag cgatctcgcc gtgctgaaag agcgcaagcg cgagttctac
     2401 gacggcctca cgcgctggaa atcggagttc gcgcagtcca ccgcgggcgc cggcaagatg
     2461 atcgccgtcg aaggcgctac ctacgaggac gcgctcgtaa gggcgaacaa cctgctcctc
     2521 gccaaccagt ggggcgacgg cctgtcgttg tggccggcga cgaaggaacg cgtggactgg
     2581 gtgctccggg gctccgcgct tccacgcacg cacgtgctcg gcaagttccc gccgcgcggc
     2641 ggcatcacca ccatcgggac ctgcgccatc gcgctcgcca tggcgggcgg gcggcccgaa
     2701 tacctgccgg tcctcgtcgc cgcggtggaa gcgtgcctcg aatccgaggg tttcgaccag
     2761 ttgcaggcca cttcgggcgg gccgttcccg gccgtcatcg tcaacgggcc gatcgcgaaa
     2821 cagatccgcc tcaactcggg cttcggtctc ctcggtcccg acccgcagcg gcccgcgggg
     2881 gcgagcatcg gtcgcgcgtt gcgcctgctg cagcagaacg tgggcggcgc gctgccgggc
     2941 atcgggacga tggcgatctt cggcgccatg cgctacacca acgcggtctt cgccgaggac
     3001 gaggacgggc tcccgccggg ttggcagccg cacgcgaccc agcgccacgg gttcgcgccg
     3061 ggcacgaact cggtctcggt cgtcttcgcg agcggggtga ccaacgtccg gcgccgcggc
     3121 gcgaagaagg agaccccgga ggaagacgcg ctgaacggca tgtggcgcat ggcggatttc
     3181 atgcgcaccc ccaacctcgc ggtgctccac ggatgggaaa agggcacacc cggcatcatg
     3241 atgtttgctc cggtggttgc gcggcgcatg gcggagctcg gctggaccca gcgctcgata
     3301 cgcgagttcc tgtgggagaa ctcgaagatc ccgatggagc agatgcgccg cgccggtgcg
     3361 ccggcctgga tcgagatcga cgccaacccg gtgacgcgcg cgagcatcaa cctcga
//
LOCUS       DBNGWZ010000571         2445 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2099195, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000571 DBNGWZ010000000
VERSION     DBNGWZ010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2099195"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..814
                     /locus_tag="AC16CX_17850"
     CDS             242..814
                     /locus_tag="AC16CX_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014410.1"
                     /translation="MLYMEEGIKPIRRVVTGNDAQGRSRVLFDSAAPNVNANAFKKGT
                     GMTDIWVFNSCPAVISGERDDGNLPFHFEPPATGGHLRIVQSDPKPKDYDPADDKYIV
                     APHPPRKTEGGTWERGGQNLFTTRMHKSETLDYGILLAGERILILDDGEYVLKPGDTV
                     VQIGSWHAWGNPNEGSQMAFVMMGATFDDE"
     gene            828..1379
                     /locus_tag="AC16CX_17855"
     CDS             828..1379
                     /locus_tag="AC16CX_17855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014411.1"
                     /translation="MNEESKPVRRIVVMDENDRSRAIADGPSPDVRIDPARPGFASTR
                     IWATDRTPATIKGVREALQLPHAIEPPAGGSVCRVVTFPPDDSWRGKVGAREVKQYFA
                     AMGSPGASTYSPRAPHPYMQKTRTLDFCLILEGEITLVLDTQEVVLKAGDTVVQRGTN
                     HAWSNRSNRPCVIAFSSHDAAPY"
     gene            1515..2441
                     /locus_tag="AC16CX_17860"
     CDS             1515..2441
                     /locus_tag="AC16CX_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014412.1"
                     /translation="MLVIPDTVQTAIAQNYPTKPIRFIVGPGPDVLPRLIGQKLIDDW
                     GQQVVVDQRPGAGGIIAADTAAKAAPDGYTLLLTTGAYTVNAVYYTKLPYDLVRDFAP
                     VSLLATIQFLLVVHPSVPVKSVEELLQLARAKPGQLNCASSCTGTTAHLGCEMLKSMG
                     RIDIVHVPYKGVAPAITELLGGQVQMFFAVMQAGLPHVRAGKLKALAVSGAKRAPAAP
                     ELPTVAEAGIPGFAFDSWNGVHVPARTPKPVTAKLNAELVKTIKLPDVQKRMLDLGLE
                     PVGSTPEDFAAFVKADIARWARVVKDAGVRTE"
BASE COUNT          491 a          745 c          780 g          429 t
ORIGIN      
        1 caaaggatct gagcggcgcg ctgaagcaag ccatcgcgat gaacaaaccc gtggtcgtgg
       61 acgccgtcac cgacatatgc gcgatcgcgc cgcatccgtg gacaccgagc ggcagggact
      121 ttcacaccta tcagagggga tgagctgtga gccatcagct ttcagcggtc agcgagaaca
      181 ccgctgcgtt tcaagctgag agccgatgac cgaccgctga aggctggata gtaagaacgg
      241 ggtgttgtat atggaagagg gcatcaagcc gatccggcgg gtggtgaccg gaaacgacgc
      301 gcaggggagg tcgcgggtgt tgttcgacag cgcggcgccc aacgtcaatg ccaatgcctt
      361 caaaaagggc accggcatga ccgacatctg ggtttttaac agctgcccgg ccgtcatatc
      421 gggcgaacgc gacgatggca atctgccgtt ccacttcgag ccgccagcga ccggcgggca
      481 tctacgcatc gtccagtcgg atccgaagcc gaaggactac gacccggccg acgacaaata
      541 catcgttgcg ccccatccgc cgaggaagac cgaaggaggc acgtgggagc gcggtggcca
      601 gaacctcttt accacgcgca tgcacaaatc ggaaacgctc gactacggca tcctgctggc
      661 gggcgagcgc atcctcattc tcgacgacgg cgagtacgtg cttaaaccag gcgacaccgt
      721 ggtgcagatc ggcagctggc acgcgtgggg caatcccaac gagggcagcc agatggcgtt
      781 cgtcatgatg ggagcgacgt tcgatgatga atgatgagtg atgaatgatg aatgaagaat
      841 caaaaccggt gcgcaggatc gtcgtgatgg atgagaacga cagatcgcgg gcgatcgcgg
      901 acggcccgtc accggacgtg cgcattgacc cggcgcgtcc gggctttgcg tccacccgca
      961 tctgggcgac cgacaggacg ccggcgacaa tcaagggagt gcgggaagca ctgcagcttc
     1021 cgcatgccat cgagcctccg gccggtgggt cggtttgccg cgttgtcacg tttccgcccg
     1081 atgacagctg gagaggcaag gtgggcgcca gggaggtgaa acagtatttt gctgcgatgg
     1141 gttcgccggg cgcctcgacc tattccccga gggcgccgca cccctacatg cagaagaccc
     1201 gcacgctcga tttctgcctg atcctcgagg gtgagatcac gctcgtcctc gacacccagg
     1261 aagtggtcct gaaagcgggc gacaccgtcg tgcagcgcgg gaccaatcac gcctggagca
     1321 accggtcgaa ccggccttgc gtgatcgcat tctcgtcgca cgatgccgct ccatattgat
     1381 cttgcggaaa tggttgacac actatttccg cggcgacccc tcctcgaaaa ggaggatatt
     1441 ttgatcaggg aacaatgatg gcctctggta atctctttac agttttgcgc gccgttgtcg
     1501 tcattgtttc ccctatgttg gttattcccg atacggtgca aaccgcgatc gcacaaaact
     1561 atccgaccaa gccgattcgc ttcatcgtcg ggcccggccc cgacgtcctg ccgcgcttga
     1621 tcggccagaa gctcatcgat gactggggac agcaggtcgt ggttgaccag cgccccggcg
     1681 ctggcggcat catcgccgcg gacaccgcgg cgaaagcggc gcctgacggc tatacgctgc
     1741 tcctgaccac cggcgcctat accgtgaacg cggtctacta caccaagctt ccctatgacc
     1801 tcgtgcgcga tttcgcgccg gtaagcctgc tcgccacgat acagtttctg ctggtcgtac
     1861 acccgtcggt gccggtgaaa tcggttgagg agctgctcca gcttgcgcgc gcgaagcccg
     1921 gacagctcaa ttgcgcctcc agctgcaccg gcacgactgc gcatctcggc tgcgagatgc
     1981 tgaagagcat gggccgcatc gatatcgttc acgtgcccta caagggcgtc gccccggcga
     2041 tcaccgagct gctcggcggg caggtgcaga tgttctttgc cgtgatgcag gccggcttgc
     2101 cgcacgtgag ggcggggaag ctgaaggctc tggcggtgag cggcgcgaag cgcgcgcccg
     2161 cggcccccga gctgcccact gtcgctgaag ccggcattcc cggtttcgcg ttcgactcgt
     2221 ggaacggggt gcacgtcccc gcccgcacgc ccaaacccgt taccgcaaag ctcaacgcgg
     2281 agttggtgaa gacgatcaag ctgccggacg tgcagaaacg catgctggat cttggtctgg
     2341 aaccggtggg aagcacgccg gaagatttcg ccgctttcgt caaggccgac atcgcaagat
     2401 gggctagggt ggtcaaagac gccggcgtgc gcaccgagtg acggg
//
LOCUS       DBNGWZ010000572         2112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1722818, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000572 DBNGWZ010000000
VERSION     DBNGWZ010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1722818"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="AC16CX_17865"
     CDS             <1..1163
                     /locus_tag="AC16CX_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014413.1"
                     /translation="ALEESASMSGANLAQVVRRITLALSWPAIFATLLILFVRAIESF
                     EVPALLGMPGGVFVFTSAIYEAIHRYPSQVGLASAYGVTLLAITSVGIWFQSRLSGTG
                     HRYSTVTGKGFRPRRIDLGRWRWATAAIFIVYFLLIVAAPLLVLVWSSIQKYYSVPSM
                     AALANLTLEPYRFILNHPTFLRAVWNSLVLCLGSATIIMLVTSVICWMVVKTRIPGRW
                     LLDNLASLPIVFPGLVLGLSIMVFYLNVNVGIYGTIWILLLAYVTRFMPYGIRYNTAS
                     MVQIHKELEESAAMSGASWGSTFVRVVLPLLKPGLVAGWIYIAIVSLRELSSSILLYS
                     PGTEVVSIMIWELWENGQYVELSALGVMLIVLLFVMVQIAQIVGSRFGVREA"
     gene            1370..>2112
                     /locus_tag="AC16CX_17870"
     CDS             1370..>2112
                     /locus_tag="AC16CX_17870"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014414.1"
                     /translation="MLSVRGLNTEYATEGGEPVRAARDVSFEVPEGKLFTLLGPSGCG
                     KTTTLRSIAGLERPQSGEIALAGRTIYSSDRRVFIAPNERNIGMVFQSYAIWPHMTVF
                     ENAAFPLKVGRRRLAAKEIREKVMRVLAIVAMDALEDRPATNLSGGQQQRLALARALV
                     MGPQLLLLDEPMSNLDAKLRERMRFELKRIQRETGVTTVYVTHDQSEALALSHEIAVM
                     NEGTIMQVGTPREIYERPRSRFVADFIGRT"
BASE COUNT          336 a          707 c          656 g          413 t
ORIGIN      
        1 ccgcgctcga ggaatcggct tcgatgagcg gggcgaacct cgcccaggtg gtgcggcgca
       61 tcacgctggc gctttcctgg cccgcgatct tcgcgacgct gctgattctc ttcgtgcgcg
      121 cgatcgagtc cttcgaggtg ccggcgctgc tcgggatgcc gggcggggtc ttcgtcttca
      181 cttccgcgat ctacgaagcc atccaccgct atccgagcca ggtcgggctc gcctcggcct
      241 acggcgtgac gctgctcgcc atcacctcgg tcggcatctg gttccagtcc cggctctccg
      301 gcaccggcca ccgttactcg accgtgaccg gcaagggctt ccgcccgcgc cgcatcgatc
      361 tcggccgctg gcgctgggcg acggcggcga tcttcatcgt ctatttcctg ctgatcgtcg
      421 cggcgccgct cctggtgctg gtctggtcct cgatccagaa gtactacagc gtgccgtcga
      481 tggcggcgct cgccaatctc acgctcgagc cctaccgctt catcctcaac cacccgacct
      541 tcctgcgcgc ggtgtggaac agcctagtcc tgtgcctcgg cagcgcgacc atcatcatgc
      601 tcgtcacctc cgtgatctgc tggatggtgg tgaagaccag gattcccggc cgctggctgc
      661 tcgacaacct ggcctccctc ccgatcgtct tccccggcct ggtgctcggc ctctcgatca
      721 tggtgttcta tctcaacgtc aacgtcggca tctatggcac catctggatc ctgctgctcg
      781 cctacgtcac gcgcttcatg ccctatggca tccgctacaa caccgcctcc atggtgcaga
      841 tccacaagga gctggaggaa tcggcggcga tgagcggcgc ctcctggggc tcgacgttcg
      901 tccgcgtcgt gctgccgctc ttgaagcccg gcctggtcgc gggctggatc tacatcgcga
      961 tcgtctcgtt gcgcgagctt tccagctcga tcctgctcta cagccccggc acggaggtgg
     1021 tctcgatcat gatctgggag ctgtgggaga acggccagta cgtcgagctc tccgcgctcg
     1081 gcgtgatgct gatcgtgctg ctgttcgtga tggtgcagat cgcgcagatc gtgggcagcc
     1141 gcttcggcgt gcgcgaagcg tgactcgatt gaaaccgccg atgaacgccg atgaacacgg
     1201 atagaatcaa agaaatccgg gcactatgat cacagagaac acagagagtt gaaatgcgaa
     1261 taaccggatt aatttttgat tttcactgcg gactccgtgt tctccgtggc ttgtatctgt
     1321 cttttattgg cgttaatcgg cgtttatctg cggttgcatt tgacgtcaca tgctgagcgt
     1381 tcgcggcctc aacaccgaat acgcgaccga aggcggcgag ccggtccgcg ccgcgcgcga
     1441 cgtttctttc gaggtgcccg agggcaagct gttcacgctg ctcggcccca gcggctgcgg
     1501 caagaccacg acgctgcgct cgatcgcggg gctggagcgc ccccaatccg gcgagatcgc
     1561 gctcgcgggg cgcacgatct actcctcaga caggcgggtg ttcatcgcgc ccaacgagcg
     1621 caacatcggc atggtgttcc agtcgtacgc gatctggccg cacatgaccg tgttcgagaa
     1681 cgccgctttc ccgctcaagg tgggccggcg caggctcgcc gcgaaggaga tacgggagaa
     1741 agtgatgcgc gtgctcgcca tcgtggcgat ggacgcgctc gaggaccggc cggcgaccaa
     1801 cctctccggc ggccagcagc agcgcctcgc gctcgcgcgc gcgctggtga tgggaccgca
     1861 gctgctgctg ctggacgagc cgatgtcgaa cctcgacgcg aagctgcgcg agcgcatgcg
     1921 cttcgagctg aagcgaatcc agcgcgaaac cggcgtcacc acggtctacg tcacccacga
     1981 ccagtccgag gcgctcgcgc tctcgcacga gatcgccgtg atgaacgagg gcacgatcat
     2041 gcaggtcggc acgccgcgcg agatctacga gcggccgcgc agccgcttcg tcgccgactt
     2101 catcggcagg ac
//
LOCUS       DBNGWZ010000573         5883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1971223, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000573 DBNGWZ010000000
VERSION     DBNGWZ010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1971223"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..760
                     /gene="tatC"
                     /locus_tag="AC16CX_17875"
     CDS             2..760
                     /gene="tatC"
                     /locus_tag="AC16CX_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR3014415.1"
                     /translation="MTEDTFISHLIELRDRLLRSLVAIAAVMIVLAIWPGIGGVYDLL
                     ARPMMQSLPEGTRMIATGVVSPILVPLKVTLLAAFMVALPYVLYQAWAFVAPGLYAHE
                     KRIALPLVVSSTLLFLAGVAYCYFVVFGLIFQAIHSYAPQAITPAPDIEAYLNFAITM
                     FLAFGITFEIPLVQVVLVRADVVTSEKLKDVRPYVIVGAFVVAAIVTPPDVLSQLLLA
                     IPMCLLYEVGIVATRLFGKPARPESNERETSIEG"
     gene            complement(824..1975)
                     /locus_tag="AC16CX_17880"
     CDS             complement(824..1975)
                     /locus_tag="AC16CX_17880"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR3014416.1"
                     /translation="MRKLWLVFSQTATICLAVLFVVSTLRPDLLTWNTRGNVVTVKEP
                     EPDSPAHAGASFRDAARKAMSAVVNIFTSQEVKRSRHPLMDDPLFRYFFGDQFGAGPQ
                     RREGLGSGVIVSEQGYILTNHHVIESVDQIEIALADSRKVPARVVGTDPETDLAVLKI
                     ELKKLPSITFARADLLRVGDIVLAIGNPFGIGQTVTLGIVSGLGRSHLGITTFENFIQ
                     TDAAINPGNSGGALVDASGNLVGINTAIYSQTGGSMGIGYAIPVSTARLVMEQIIQKG
                     SVTRGWIGVGVQDITKELAESFKLSATGGVLITQVERGGPADKAGVKLGDVLLAVNGK
                     AVADTTGMLNMIAVLQPGEQARLRVSRNQAESELAVTVGQRPRPPQRKE"
     gene            2190..2789
                     /gene="petA"
                     /locus_tag="AC16CX_17885"
     CDS             2190..2789
                     /gene="petA"
                     /locus_tag="AC16CX_17885"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HIR3014417.1"
                     /translation="MADKELDQGKRRFLIAATSVAGGIAGVATAVPFVASMLPSERAR
                     AAGAPVEADIGNLTPGEMTRVEWRGKPVWIVRRTKEMLESVKQNDNKVADPKSERKKE
                     LTPEYARNEFRSIKPEYLVVVGICTHLGCSPVDKFKAQPEAFEADWKGGFYCPCHGSL
                     FDLAGRVYKNKPAPDNLEVPPHTYLSDTKIVIGQDSKGA"
     gene            2794..4188
                     /locus_tag="AC16CX_17890"
     CDS             2794..4188
                     /locus_tag="AC16CX_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR3014418.1"
                     /translation="MAVAEKQPVTGAGPFNGVLTWIDQRFPLMSLWRDHLAEYYAPKN
                     FNFWYYFGSLAMLVLVIQIVTGIFLAMHYKPDAAQAFASVEYIMRDVPGGWLIRYMHS
                     TGASAFFIAVYLHMFRGLLYGSYRRPRELIWIFGMLIYFCLMAEAFFGYLLPWGQMSY
                     WGAQVIINLYDAIPVIGPTLSEWIRGDYVVSDATLNRFFSFHVVAIPLVLLALVAAHI
                     MALHEVGSNNPDGIEIKKLKDPKTGRPLDGIPFHPYYTVKDTLGVVVFLIVFSVIMFF
                     MPEFGGYFLEYNNFIPADPLKTPPHIAPVWYFTPYYSILRATTENFMWVLAAGVIAYS
                     ILLARTLKSGAVRIGVALIAVLLLIGFFTIEPKVWGVVLMGVSVMIYFLLPWLDQSPV
                     KSIRYKGPLVKSALALFVIAFFVLGYLGTQTVTEGRTLMAQIFTLVYFAFFLLMPWYT
                     RMDKTRPEPERVTS"
     gene            4200..4973
                     /locus_tag="AC16CX_17895"
     CDS             4200..4973
                     /locus_tag="AC16CX_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HIR3014419.1"
                     /translation="MKSARMLLFVLFAAPALAIAAEEVKLDRMPADVNPADPVSLQRG
                     AQVYVNYCLGCHGASYMRYNRLQDLGLTEQQILDNLIFTAAKVGDLMRTAMDPKDAKE
                     WFGAPPPDLTVIARSRSSAAGSGADWLYTYLRSFYRDPSRPTGWNNTLFPNVGMPHVL
                     WQLQGEPVLKTEVRAIPRGSKGEVEKFEVQKLVLEKPGAMSVVEYDRLAADLVNFLVY
                     MGEPARQSRVQLGIYVLLFLGVLFVLAYLLKKEFWKDVH"
     gene            5043..5642
                     /locus_tag="AC16CX_17900"
     CDS             5043..5642
                     /locus_tag="AC16CX_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3014420.1"
                     /translation="MMTLYSGTTCPFSQRCRIVLYEKGMDFQIVDVDLYNKPEDLAVM
                     NPYNQVPVLVERDLILYESNIINEYIDDRFPHPQLMPADPVMRARARLFLFRFEHEMF
                     CHIEAIEKGTQKQADKGRAIIRDNLTQIAPVFTKQKYMLGEEFTMLDVAIAPLLWRLD
                     HYGISLPKACAPLMKYAERLFSRPAFIDALTASEKVMRR"
     gene            5757..>5883
                     /locus_tag="AC16CX_17905"
     CDS             5757..>5883
                     /locus_tag="AC16CX_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HIR3014421.1"
                     /translation="MAEKPTKPYLIRAIHEWCTDSGLTPYLSVQVNAETRVPAEQV"
BASE COUNT         1034 a         1883 c         1820 g         1146 t
ORIGIN      
        1 aatgacggaa gacaccttta tttcccatct gatcgagttg cgggaccgcc tcctgcgctc
       61 gctcgtcgca attgcggcgg tgatgattgt gctcgcgatc tggccgggaa tcgggggcgt
      121 ctacgacctg ctcgcccggc ccatgatgca atcgctgccg gaaggaaccc ggatgatcgc
      181 gaccggcgtc gtgtcgccga tactcgtccc gctcaaggtt acgctgctcg ccgccttcat
      241 ggtggcgctt ccctacgtgc tgtaccaagc ttgggcgttt gtcgcccctg gattgtacgc
      301 gcacgaaaaa cgcatcgcct tgccgcttgt cgtcagcagc actttgttgt ttctggccgg
      361 cgtggcctat tgctacttcg tcgtcttcgg gttgatattc caggcgatcc acagctacgc
      421 gccgcaggcg atcaccccgg cccccgacat cgaggcgtac ctcaatttcg ccatcacgat
      481 gttccttgca ttcgggatca cgttcgagat cccgctggtc caggtggtgc tcgtgcgcgc
      541 ggacgtcgtc acctccgaaa agctgaagga tgttcgtccc tacgtgatcg tcggcgcgtt
      601 cgtcgtcgcg gcgatcgtga cgccgcctga cgttctgtcg cagctgctcc tcgcgatccc
      661 gatgtgcctg ctctacgagg tcgggatcgt cgcgacgcgc ctgttcggga agccagcgcg
      721 acccgagagc aacgagcggg aaaccagtat tgagggttga gtgcttggcg ccgaaggctg
      781 tgcgccgtgc ttgactcggg actcaaaaaa ataacggcga gggttattcc ttgcgttgcg
      841 gcggtcgcgg ccgctggccg acggtgacgg caagctcgct ttcggcctgg ttgcgcgaca
      901 cccgaagcct cgcctgctcg cccggttgca gcacagcgat catgttgagc atgccggtag
      961 tgtcggcgac cgctttgccg ttgaccgcga gcagaacatc gcccagcttc acgcccgcct
     1021 tgtcggcggg tccgccgcgc tccacctggg tgatcagcac gccgcccgtc gcggacagct
     1081 tgaacgattc ggcgagctcc ttggtgatgt cctgcacgcc gacgccgatc cagccgcgtg
     1141 tcaccgagcc cttctggatg atctgctcca tcaccagcct ggcggtgctc accgggatgg
     1201 cgtaaccgat gcccatcgag ccaccggtct gcgagtagat cgcggtattg atgccgacca
     1261 ggttgccgct cgcatccacc agcgcgccac ccgaattgcc ggggttgatc gcggcgtcgg
     1321 tctggatgaa gttctcgaac gtggtgatgc cgagatggct gcgaccgagg ccgctgacga
     1381 ttcccagggt gacggtctgc ccgatgccga acgggttgcc gatcgcgagc acgatgtcgc
     1441 cgacgcgcaa gaggtccgcc ctggcgaagg tgatcgaagg cagcttcttc agctcgatct
     1501 tgagcacggc aaggtccgtt tccggatcgg tgccgaccac gcgcgccggc accttgcgcg
     1561 agtcggcgag cgcgatctcg atctggtcca ccgactcgat tacgtggtgg ttggtgagga
     1621 tatagccctg ctcgctgacg atgacgcccg accccaggcc ttcgcggcgc tgtggccccg
     1681 cgccgaactg gtctccgaag aaatagcgga acagcgggtc gtccatgagc gggtggcgcg
     1741 agcgcttgac ttcctggctc gtgaagatgt taaccactgc ggacatcgcc ttgcgcgccg
     1801 catccctgaa ggaggcgccg gcgtgcgccg gcgagtccgg ctccggctcc ttcacggtca
     1861 ccacgttgcc gcgcgtgttc caggtgagaa ggtcggggcg cagcgtcgag acgacgaaca
     1921 agaccgcgag gcaaatcgtc gcggtctggg aaaatacgag ccagagcttg cgcatggaat
     1981 tggcagtcgc aggggaggcg gtcggggctg gaatgggtat tttcttcggg cgcctcgaat
     2041 catagcaaac cggtgaccga cagccccgag tcccgacggg cggctccccc tgtaattcgc
     2101 tgattacagg aaacttttcc acattttgct taagtttcgc cgggcctttg tgtagaatgg
     2161 cgatttttcg ggcggcgggg aagaatccca tggcagacaa agagttggat cagggcaagc
     2221 ggcgtttcct gatcgccgcc accagcgttg cgggaggcat cgccggcgtc gccaccgccg
     2281 tgccgttcgt cgccagcatg ctgccttcgg agcgcgcgag ggcagcgggc gcgccggtcg
     2341 aggccgacat cggcaacctg acccccggcg agatgacgcg cgtcgaatgg cgcggcaagc
     2401 cggtgtggat cgtacgccgc accaaggaga tgctggagtc cgtcaagcag aacgataaca
     2461 aggtcgccga tccgaagagc gaacgcaaga aggagctcac gcccgagtac gcgcgcaacg
     2521 agttccgctc gatcaaaccc gagtacctgg tggtcgtcgg catttgcacg catctcgggt
     2581 gctcgccggt ggacaagttc aaggcccagc ccgaggcatt cgaggccgac tggaagggcg
     2641 gcttctactg cccgtgccat ggctcgctgt tcgatctcgc aggccgcgtc tacaagaaca
     2701 agccggcccc ggacaacctc gaagtcccgc cgcacaccta tctttccgat accaagatcg
     2761 tgatcgggca ggacagcaag ggagcctgac atcatggcgg tcgcagaaaa gcaaccggtc
     2821 accggggccg ggccgttcaa cggcgtactc acctggatcg accagcgctt tccgctgatg
     2881 tccctgtggc gggaccacct cgccgagtat tacgcgccga agaacttcaa tttctggtac
     2941 tacttcggct cgctcgcgat gctggtgctg gtgatccaga tcgtcaccgg catcttcctc
     3001 gccatgcact acaagccgga tgcggcgcag gccttcgcct cggtcgagta catcatgcgt
     3061 gacgttccgg gcggctggct gatccgctac atgcactcga ccggcgcgtc cgcttttttc
     3121 atcgccgtct acctgcacat gttccgcggg ctcctctacg gttcctatcg caggccgcgc
     3181 gaattgatct ggatcttcgg catgctgatc tatttttgcc tgatggcgga ggcgttcttc
     3241 ggctacctgc tgccgtgggg gcagatgtcg tactggggcg cgcaggtgat catcaacctg
     3301 tatgacgcta ttcccgtgat cggtcccacg ctctcggaat ggatccgcgg cgattacgtg
     3361 gtgtcggacg cgaccctgaa ccgtttcttc tcgttccacg tggtcgcgat tccgctggtg
     3421 ctgctcgcgc tcgtcgccgc gcacatcatg gcgctgcacg aggtggggtc gaacaatccg
     3481 gatggcatcg agatcaagaa gctgaaggac cccaagaccg gccgcccgct ggacgggatc
     3541 ccgtttcacc cctattacac ggtcaaggac acgctgggcg tggtggtgtt cctgatcgtg
     3601 ttcagcgtga tcatgttctt catgccggag ttcggcggct atttccttga gtacaacaac
     3661 ttcatcccgg ccgatccgct caagaccccc ccgcacatcg cgccggtgtg gtacttcacc
     3721 ccgtactact cgatcctgcg cgccaccacc gagaacttca tgtgggtgct cgcggcgggc
     3781 gtcatcgcct attcgatcct gctcgcccgg accttgaaaa gcggtgcggt acgcattggc
     3841 gtcgcgctca ttgcagtgct gctcctgatc ggctttttca ccatcgaacc gaaggtttgg
     3901 ggcgtggtgc tcatgggcgt ttccgtgatg atttatttcc tgctgccgtg gctcgaccag
     3961 agccccgtca agtccatccg ctacaagggt ccgctggtga agagtgcgct cgcgctgttc
     4021 gtgatcgcct tcttcgtcct gggttacctc ggcacccaga ccgtgaccga ggggcgcacc
     4081 ctcatggcgc agatcttcac gctggtctat ttcgcgttct tcctgttgat gccgtggtac
     4141 acgcggatgg acaagacccg gcccgagccc gagcgggtga cgtcatgacg ggcgcgtcga
     4201 tgaagagcgc cagaatgctc ctcttcgtgc tgttcgccgc cccggcgctc gccatcgcgg
     4261 cggaggaggt caagctcgac cgcatgccgg ccgacgtcaa cccggccgac ccggtttcac
     4321 tccagcgcgg cgcgcaggtg tacgtcaact actgtctcgg ctgccacggc gcgtcctaca
     4381 tgcgctacaa ccggttgcag gacctggggc tcaccgagca gcagatcctg gacaacctga
     4441 tcttcaccgc tgccaaagtc ggcgacctca tgagaaccgc gatggacccc aaggacgcca
     4501 aggaatggtt cggcgcgccg ccgcccgacc tcaccgtgat cgcgcgctcc cgttcctcag
     4561 ccgcgggcag cggggcggat tggctctaca cctatctgcg cagcttctac cgggatccct
     4621 cgcgccccac cggctggaac aacacgttgt tccccaacgt cggcatgccg cacgtgctct
     4681 ggcagctcca gggcgagccg gtcctgaaga ccgaagtgcg ggccatcccc cgtggcagca
     4741 agggggaggt ggaaaagttc gaggtgcaga agctcgtgct ggaaaagccc ggcgccatga
     4801 gcgtggtcga gtacgaccgg ctggcggccg acctcgtgaa tttcctggtt tacatgggcg
     4861 agcccgccag gcagagccgc gtgcagctcg gcatctacgt gctgctgttc ctgggcgtgc
     4921 tgttcgtgct cgcctacctg ctcaagaaag aattctggaa agacgtgcat tgaagctcac
     4981 agctgtcagc gatcagctga cagccaatgc ctgaccgctg atagctgacc ccgctgactc
     5041 ctatgatgac cctgtattcc ggcaccacct gcccgttcag ccagcgctgc cgcatcgtgc
     5101 tctacgagaa gggcatggat ttccagatcg tggatgtgga tctctacaac aagccggaag
     5161 acctggcggt gatgaacccc tacaaccagg tgccggtgct ggtcgagcgc gacctcattc
     5221 tctacgagtc gaacatcatc aacgagtaca tcgacgaccg cttcccgcac ccccagctca
     5281 tgccggcgga tccggtgatg cgcgcgcgcg cgcgcctgtt cctgttccgg ttcgagcacg
     5341 agatgttctg ccacatcgag gcgatcgaga agggcacgca gaagcaggcc gacaaggggc
     5401 gcgccatcat ccgcgacaac ctgacccaga tcgcgcccgt gttcaccaag cagaaataca
     5461 tgctgggcga ggagttcacc atgctcgacg tggcgatcgc gccgctcctg tggcggctcg
     5521 accattacgg catctcgctg cccaaggcgt gcgcgccgct catgaagtac gccgagcggc
     5581 tgttcagccg gcccgccttc atcgacgcgc tcaccgcctc cgagaaagtg atgcgcaggt
     5641 aagccgtgat tggtgattcg tgattagtga ttggaacaga gccgccaggt ttaaggccat
     5701 gacgagtaaa ctaccagtca ccagtcacaa gtcaccagtc acgaaattct aagccgatgg
     5761 cggaaaagcc caccaaaccg tacctcatcc gggcgatcca cgagtggtgc acggacagcg
     5821 ggctgacgcc gtacctctcc gtgcaggtga acgccgagac ccgggttccg gcggagcaag
     5881 tga
//
LOCUS       DBNGWZ010000574         5389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_588920, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000574 DBNGWZ010000000
VERSION     DBNGWZ010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_588920"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16CX_17910"
     CDS             <1..219
                     /locus_tag="AC16CX_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3014422.1"
                     /translation="RKTKAVFDAIGAGHGTRAQAALRFVLSNPDVSAAVIGSAELHHI
                     DEALRAAEMGPLPSQVLARLDGLYESDF"
     gene            complement(508..882)
                     /locus_tag="AC16CX_17915"
     CDS             complement(508..882)
                     /locus_tag="AC16CX_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR3014423.1"
                     /translation="MNIVDSSGWLEYFADGPNAAFFAPAIETTRELLVPTLSLYEVFK
                     RVLQQRGEGEALQAVALMQQGEIVDLTASIALTAAKASIERRLPMADSIMLITAQTHG
                     ATFWTQDADFDGVEGVKYIAKR"
     gene            complement(879..1085)
                     /locus_tag="AC16CX_17920"
     CDS             complement(879..1085)
                     /locus_tag="AC16CX_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR3014424.1"
                     /translation="METVTISPKFQVVIPKDIRDTLKLAPGQKVQAIAYENRIELIPL
                     RPMKKMRGFLKGMNTDVPREEDRA"
     gene            1273..1449
                     /locus_tag="AC16CX_17925"
     CDS             1273..1449
                     /locus_tag="AC16CX_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014425.1"
                     /translation="MAGPLPDMTLEENMTVVVQPNVITRDHKAGVQVGELIRITRTGF
                     ERLHRAKRGLFRVG"
     gene            complement(1752..2723)
                     /locus_tag="AC16CX_17930"
     CDS             complement(1752..2723)
                     /locus_tag="AC16CX_17930"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014426.1"
                     /translation="MKTFGTVAAGIIALSCAAAVGAQQPYPSKPIRLIVGFAPGGNTD
                     TVARLVGQKLGERLGTQVVIDNRGGAGGTIGTEIAARANPDGYTLTMGTTTTHAIAVA
                     AYPDLRYDPVKDFAPAALVAIAPYLLVVHSSLPPRNLKEFVALVKSQPGKLTYGSAGT
                     ATTTHLAMVNLATRAGLDMTHVPFKGNGPATTAVVGGEVKILFGAMPPLLPHLPGGRV
                     RAIAVSSGKRSASLPEVPTVAESGFPGYEVTLWLGFFAPRGTPPAVMKRLETELVNVV
                     NSPDLREQMLRQGLEAHGATAAQLGKLVRAEIEDYKKSFKTAGIKLQ"
     gene            2898..3791
                     /locus_tag="AC16CX_17935"
     CDS             2898..3791
                     /locus_tag="AC16CX_17935"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HIR3014427.1"
                     /translation="MTTRLAFIGFGEVASAFSAALAARGAEIAAYDVLMGQAGGIDRL
                     KARAGATNVAFRGLPEAVHGAHYVLSTVTTSAARETARQCVPHVGPGLTYVDLNATDP
                     AVKLEIERIIAPSGAAFVEGAVLGAVGVTGAKTEILLGGPHGRRAERELGGGLGLNVR
                     FYSEDIGKASMFKMLRSVFSKGMEALLIEFLVAGERAGIRDDLWREVTELFASHPFEK
                     TASNWVRSHATAHARRYHEMKQVAGVLRQLGVEPVVTAATEAFFERSGSLGLGERFQK
                     TPATMDELVKYLDRRLAAPAR"
     gene            3832..4593
                     /locus_tag="AC16CX_17940"
     CDS             3832..4593
                     /locus_tag="AC16CX_17940"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3014428.1"
                     /translation="MKQNRMKEKLRAGEPVYGVSVMIPSPQIVEMVAAAGFDWVLLDC
                     EHGTLTIESVELMAMAAEASGITAIARPATRSPEHILQVLDRGVMGVQVPHVSTAEDA
                     REVVAAVKYHPLGSRGLAAGTRAAVYDAHGTLADYVKAANDATLIAIQLEERTAIDNI
                     DALLKVDDIDVYFIGPSDLSQSMGHPGNPRAPAVVAAIDGSFRKMVAAGRTPGTPATA
                     ENVREVLDKGVRYIYTHLPRLLAGSARAYLKAARG"
     gene            complement(4866..>5389)
                     /locus_tag="AC16CX_17945"
     CDS             complement(4866..>5389)
                     /locus_tag="AC16CX_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014429.1"
                     /translation="ATFPYGTSGTGGTPHLAGELLKQRTGIALEHIPYKGGGQAIIDV
                     LGGQIPLVFTAVATAQQYVKAGRLIGLGVPGARRSAALPDVPTFIESGQPGFDVTSWV
                     SILAPAKTPRPVIEKLHREIAAVLLTDTVRERYLVLGIEPVGSTPEQFADQIRADLAR
                     WEKVVRAANIRLE"
BASE COUNT          913 a         1690 c         1877 g          909 t
ORIGIN      
        1 cgcaagacga aggccgtgtt cgacgcgatc ggcgccgggc atggaacgcg cgcacaggcc
       61 gcgctccgct tcgtgctgtc gaacccggac gtatcggcgg ccgtaatcgg cagcgcggag
      121 ttgcaccata tagacgaggc gctgcgggca gcggagatgg ggccgctgcc gtcgcaggtc
      181 ctggcgcggc tggatgggct gtacgagagc gacttctgat agtcggtgat gagtgatggg
      241 tgatgggtga tgagtgagga gactcccgca gtgcggagtg agctaaagct tcaccgccaa
      301 ggccgcgggg gacgcgaagg aaaagaaaag cttcaccgcc aaggccgcga aggacgcaaa
      361 gtattacaaa taaaagccac gaatctccac agtccattca ccattcacta ttcaccattc
      421 accgcttttg agagggcgcg aaagaaccgc gccaagtgtg gcctccagga ggcgctcttg
      481 ggaatacagg ctcctggtaa tagtttacta acgcttcgcg atgtatttca caccttcaac
      541 gccgtcaaaa tcggcgtcct gcgtccagaa ggtggcgccg tgggtctgcg cggtgataag
      601 catgatgctg tcagccatcg gcaggcggcg ttcgatgctc gcctttgcgg cggtgagtgc
      661 aattgatgcc gtcagatcga cgatttcccc ctgctgcatc aacgctaccg cctgcagggc
      721 ctcaccctcg ccgcgctgct ggagcacgcg cttgaacact tcgtagaggc tcagcgtcgg
      781 gaccagcagt tcgcgcgtcg tctcgatcgc cggggcgaag aacgcggcgt tggggccgtc
      841 ggcgaagtac tccagccagc cggaagagtc gacgatgttc acgcgcggtc ttcctcgcgc
      901 ggcacgtcgg tattcatgcc cttgaggaat ccccgcatct tcttcatcgg ccgcagcggg
      961 atcagctcga tgcgattttc gtaggcgatg gcctgcactt tctgtcccgg ggcgagcttg
     1021 agcgtgtcgc ggatgtcctt ggggatgacg acctggaact tgggcgagat cgtgacggtt
     1081 tccatggctc tcctatcgat caataaatcg tatgacgatt atcgtatcga tgggttctgg
     1141 ggtcaagcgc gcggcgggca aagccccgca atgaatgcaa caactacatg attgttatag
     1201 ctctatttcg ggatctgacc ctaaggtttc ggcggcggct atttcctgcc catcctcggc
     1261 acccggagcc ggatggccgg cccgctgccc gacatgacgc tggaggagaa catgacggtg
     1321 gtggtgcagc ccaacgtgat cacccgcgac cacaaggcgg gggtgcaggt cggtgaattg
     1381 attcgcataa cccggaccgg cttcgagcgg ctgcacaggg cgaagcgggg gttgttccgc
     1441 gtcggttaga cgcaaagctt caccgccaag gccgcggggg acgcgaagga aaagaacaga
     1501 ctcaccgcca aggccgcgaa gacatcgcca agggaggtaa caggaagcgc tccggggatt
     1561 acgggcgtct agtgggtttc ctttagacgc aggttttccg gcccaaaatc gaggttctaa
     1621 gcgctcaata gcggtcctat tgagcgcttt gtgtgtttct gcttcaatgc gttgtggtgg
     1681 tccgacccgg acatggaatc gaggcgctgc cgaagtagaa gcgaaagaca gggccgcgtc
     1741 cacttttctg cttattgcag cttgatgccc gccgtcttga acgacttctt gtaatcctcg
     1801 atctcggccc gtaccagctt ccccagctgg gccgcagtgg cgccatgcgc ttccaggccc
     1861 tgccgcagca tctgctcccg caggtccggg gagttgacga cgtttaccag ctcggtttcc
     1921 aggcgcttca tcacggcagg cggcgtgccc cgcggcgcga agaagcccag ccacaacgtc
     1981 acttcgtagc cggggaatcc ggactccgcg acggtcggca cctcgggcag cgaggccgag
     2041 cgcttgccgg aactgaccgc gattgcacgc acccggccgc cgggcagatg cggcagcaac
     2101 ggcggcatgg cgccgaacag gatcttcacc tcgcctccga ccacggcggt ggtggcgggc
     2161 ccgttgccct tgaacggaac gtgggtcatg tcgagccccg cgcgcgtggc gaggttgacc
     2221 atggcgaggt gcgtggtggt ggccgtcccg gcggagccgt aggtgagctt gccgggctgc
     2281 gacttgacca gcgcgacgaa ctccttgaga ttgcgaggcg gcaggctgga gtgcaccacc
     2341 agcaggtagg gcgcgatcgc gacgagcgcg gccggggcga agtccttcac ggggtcgtag
     2401 cgcagatcgg gataggcggc gacggcgatg gcgtgcgtcg tcgtcgtgcc catggtcagg
     2461 gtgtagccgt ccgggttggc gcgcgccgcg atctcggtgc cgattgtgcc gcccgcgccg
     2521 ccgcggttgt cgatcaccac ctgcgttccc aggcgctcgc cgagcttctg gccgacgagg
     2581 cgggcgacgg tgtcggtgtt gccgcccggc gcgaatccca cgatcaggcg gatgggcttg
     2641 ctcggatacg gctgctgcgc accgacggcc gcagcgcagc tcaatgcgat gatgccggcg
     2701 gcgacggtcc cgaacgtctt catgctgcct cctcttattt catgttatgg atgccccgca
     2761 cacgctttac ctgtgctgca aaagatcgta tgttgtcgcg attacaatac tactaccgtc
     2821 cgggcggccg cccgcgaatt catgaaagaa atgcccggca tgccccggcc ccgggacggg
     2881 aatcgcggga ggatccagtg acgacgcgcc tggcgttcat cggtttcggc gaggtggcct
     2941 cggcgttctc cgccgcgctc gccgcgcgcg gcgcggaaat cgcggcttac gacgtgctga
     3001 tggggcaggc cgggggcatc gaccggctga aggcgcgggc gggggcaacg aacgtggcgt
     3061 tccgcgggct gccggaagcc gtgcacggcg cgcactacgt gctttccacc gtgacgacga
     3121 gcgccgcgcg cgagaccgcg cggcagtgcg tcccgcacgt cggcccgggg ctgacctacg
     3181 tcgacctcaa cgccaccgat cccgccgtca agctggagat cgagcgcatc atcgcgccct
     3241 ccggcgcggc cttcgtcgag ggtgcagtcc tcggggccgt cggcgtcacc ggcgcgaaaa
     3301 ccgaaatcct gctcggcggc ccgcacgggc ggcgcgcgga gcgcgagctc ggcggcgggc
     3361 tcggcctcaa cgtgcggttc tacagcgagg acatcggcaa ggcgtcgatg ttcaagatgc
     3421 tgcgcagcgt cttctccaag ggcatggagg cgctgctgat cgagttcctc gtcgccggcg
     3481 agcgcgccgg gatccgcgac gacctgtggc gggaggtgac cgagctgttc gcgagccacc
     3541 cgttcgagaa gacggcgtcg aactgggtgc gcagccacgc gacggcgcac gcgcgccgct
     3601 accacgagat gaagcaggtg gcgggcgtgc tgcggcagct gggggtggag ccggtggtca
     3661 ccgccgcgac cgaggcgttc ttcgagcggt cggggtcgct ggggctcggg gagcggttcc
     3721 agaagacgcc cgccacgatg gacgagctcg tgaaatacct cgaccggcgg ctggcggcgc
     3781 ctgcgcgctg agcggcgcgc gcccgcgcat ccaccttcaa ggaaggcacc gatgaagcag
     3841 aaccgcatga aggaaaagct gcgcgccggc gagccggtct acggcgtctc ggtgatgatc
     3901 ccctcgccgc agatcgtcga gatggtcgcc gccgccggct tcgattgggt gctgctcgac
     3961 tgcgagcacg gcacgctcac gatcgagagc gtggagctga tggcgatggc ggcggaggcg
     4021 agcggcatca ccgcgatcgc ccgcccggcg acgcgcagcc cggagcacat cctgcaggtg
     4081 ctcgaccgcg gcgtgatggg cgtgcaggtg ccgcacgtga gcaccgcgga ggacgcgcgc
     4141 gaggtggtcg cggcggtcaa gtaccatccc ctcgggagcc gcggcctcgc cgcgggcacg
     4201 cgcgcggcgg tctacgatgc gcacggcacg ctggccgatt acgtgaaggc ggcgaacgac
     4261 gcgaccctga tcgccatcca gctcgaggag cggacggcga tcgacaacat cgatgcgctg
     4321 ctgaaggtgg acgacatcga cgtgtacttc atcggcccgt cggacctgtc gcagtccatg
     4381 gggcacccgg gcaatcccag ggcgccggcg gtggtggcgg cgatcgacgg cagcttccgc
     4441 aagatggtcg ccgcggggcg gacgcccggc acgcccgcga ccgcggagaa cgtgcgcgag
     4501 gtgctcgaca agggcgtgcg ctacatctac acgcacctgc cgcgcctgct ggcgggcagc
     4561 gccagggcct acctgaaggc cgcgcggggt tgacggcggg acctgggaag ataaaggggc
     4621 gcgtcccctt tttcctcaca cgcgatccga atcgagtacc gtgaacgagc tgtgagaggt
     4681 gagtactttg attgccgagg gacccgcggt gcggatttgg ggcttcgcga gaaggaacgc
     4741 ttagtcttct taaagcggcc gtttcgggcc ggaaaatgca cttctaagag tcttggcggg
     4801 cccgattatt cggctttgga atttcggatc agcgggttgc ggcggaccga ccccagaggc
     4861 cctcattact ccagcctgat gttcgcggcc ctcaccactt tctcccagcg cgccaggtcg
     4921 gcgcggatct ggtccgcgaa ctgctcggga gtgctgccga caggctcgat gccaagcacc
     4981 aggtagcgct cgcgcacggt gtccgtcagg agtaccgcgg cgatttcccg gtggagcttt
     5041 tcgatgaccg ggcgcggggt cttcgcgggc gccagtatgc tgacccacga ggtgacatcg
     5101 aagcccggct ggccgctctc gatgaacgtc ggcacgtcgg gcaacgccgc cgaccgcctg
     5161 gcgccgggca cgccgagccc gatgagcctc cccgccttca cgtattgctg cgcggtggcg
     5221 accgccgtga agacgagcgg gatctgcccg cccaggacgt caatgatcgc ctgcccgccg
     5281 cccttgtacg gaatgtgctc cagcgcaatg ccggtgcgct gcttcaggag ctcgccggca
     5341 aggtgcggtg tcccgcccgt gcccgaggtg ccgtacggaa acgtggccg
//
LOCUS       DBNGWZ010000575        21899 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1345431, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000575 DBNGWZ010000000
VERSION     DBNGWZ010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 21899)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21899)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21899
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1345431"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="AC16CX_17950"
     CDS             <1..120
                     /locus_tag="AC16CX_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3014430.1"
                     /translation="RPPRTGRNPKSGEKVQVPAKYVPHFKAGKELRERVDIKK"
     gene            365..661
                     /locus_tag="AC16CX_17955"
     CDS             365..661
                     /locus_tag="AC16CX_17955"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR3014431.1"
                     /translation="MRYLVWILKLAVFVLVLAFAVKNTGLVTVRYYFGGEWQSPLVFV
                     LLVAFCIGVAAGLSAGLAQAFRQRREIAALKRELRQINRGGTEGTEGTAGLIRG"
     gene            671..1846
                     /gene="lapB"
                     /locus_tag="AC16CX_17960"
     CDS             671..1846
                     /gene="lapB"
                     /locus_tag="AC16CX_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HIR3014432.1"
                     /translation="MEIEYWWLLALPLFFALGWLAARIDIKQLLLESRALPMSYFKGL
                     NFLLNEQPDKAIEAFIEVVKVDPQTIELHFALGSLFRRRGEVERAIRMHQNLVERPDL
                     APERKLAALFELAQDYFKAGLLDRAEELFLKLDTTAYAEQALRFLLEIFEQEKDWHKA
                     IGIAGKLEAVTGRSHQKEIANFCCELAINEMMHSRPDAARPHLAQALAHHRLCVRANM
                     LLGDLEAAAGRPEAAIEDWKRIESQNPPYLALVAERLYNAFKQRGKIEEGVNLLRGYL
                     DKYPSLDLLNVVFQGTLESRGAEPAYRLMRDELRRMPTLLGLDKLLEAQLLEAPLDRR
                     RDLELVKQLVNQQTRGLAMYKCDNCGFRARQYYWHCPACAAWETYSPRRTEEARLPA"
     gene            1843..2538
                     /gene="pyrF"
                     /locus_tag="AC16CX_17965"
     CDS             1843..2538
                     /gene="pyrF"
                     /locus_tag="AC16CX_17965"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR3014433.1"
                     /translation="MNDPRIIVALDYPGAREALELADRLDPALCRLKVGAELYTAAGP
                     RFVEQLLQSGFAIFLDLKYHDIPNTVAGACVAAADLGVWMINVHGLGGRAMLLAAREA
                     LSGRAARPKLIAVTLLTSIGEAEATEIGLAGNPQEAVARLARLAQASGLDGVVCSPQE
                     AAALRRQFGKEFCLVTPGIRPAETAQDDQQRIATPRQAIANGADYLVIGRPITRAPDP
                     LAALRRICDEIAR"
     gene            2601..3596
                     /gene="rfaE1"
                     /locus_tag="AC16CX_17970"
     CDS             2601..3596
                     /gene="rfaE1"
                     /locus_tag="AC16CX_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HIR3014434.1"
                     /translation="MAAGTIKPKRGASASSLIPHPSSLKNARVLVVGDAMLDRYWVGE
                     VERISPEAPVPIVRIGREEERAGGAANVARNAAALGAQVSLLSVVGVDEPGARLESLL
                     KRERVNAQLHRDPGIRTTVKLRVIAGRNQQLLRVDFETLPNHEVLASKLRDFGRMLAH
                     VDVVILSDYGKGGLAHIVKMIGMARRAGKTVLVDPKGQDYSRYRGATLITPNRAEFRQ
                     VAGPWKGERELTECAQRLRRKLDAQALLVTRGEEGMTLFQDGTRFHVATQAREVSDVS
                     GAGDTVIATLGVALASGARMRDAVRLANRAAGIVVGKFGTAVVTPKELFGSHSDK"
     gene            3689..4684
                     /gene="rfaD"
                     /locus_tag="AC16CX_17975"
     CDS             3689..4684
                     /gene="rfaD"
                     /locus_tag="AC16CX_17975"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HIR3014435.1"
                     /translation="MSYVVTGAAGFIGSNLVKALNERGITDILAVDDFKDGGKFINLA
                     DCEIADYLDKGEFLAKLEDGDFDGQISVIFHQGACSDTTETDGRYMMENNYRYSHKLL
                     DYCIGEEVHLIYASSAAIYGASREFRETRECEAPLNVYGYSKFLFDQLVRRRYEERTA
                     QVVGLRYFNVYGERESHKGRMASVAHHFFSQYRGTGHVRLFAGSGGYGDGEQRRDFVS
                     VEDCVKVNLFFLDHPDRSGIFNVGTGAAQSFHDVAVATVNACRKARAETPLTLTAMRE
                     RSIIQYIPFPDDLRGRYQSYTQADISALRAAGYGAPFLTVEQGVARYCQKLLARG"
     gene            4773..5681
                     /gene="cysM"
                     /locus_tag="AC16CX_17980"
     CDS             4773..5681
                     /gene="cysM"
                     /locus_tag="AC16CX_17980"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HIR3014436.1"
                     /translation="MDTRPIAGFSTLEDFVGNTPLVRLKRLPGRTSNTLLAKLEGNNP
                     AGSVKDRPALSMIQRAQQRGDIKPGDTLIEPTSGNTGIALAMCAAMMGYRMILVMPEH
                     LSLERRQTMAAFGSEIVLTPKEGGMEMARDVAEKMRDEGKGVILDQFANPDNPLSHYE
                     GTGPEIWRDTQGRITHFVSSMGTTGTIMGCSSFFKEKNPKIQIVGCQPSEGSQIPGIR
                     KWPPQYLPKICDWSRVDRVIEVSQADAEEMTRRLAREEGIFAGISSGGALWAAMQVLR
                     EVENAVIVTIVCDRGDRYLSTGVFPG"
     gene            5798..6568
                     /locus_tag="AC16CX_17985"
     CDS             5798..6568
                     /locus_tag="AC16CX_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011851735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR3014437.1"
                     /translation="MTPVLAFDIETVPDVEGLRRLHGLDNSVPDKAVAEMAFKLRRQT
                     AGTDFLPLHLHHVVVISCALRDRESFKVWSIGGESEGELIQRFFDGIDRYTPQLVSWN
                     GGGFDLPVLHYRGLIHRVKAARYWDMGEDNRDFKWNNYISRYHTRHLDLMDLLALYQP
                     RANAPLGDLAQLMGLPGKLGMEGAGVWDAYQAGKLAEIRNYCEADVVNTYLVFLRLQL
                     MRGALTDQQYKTESDVVRAMLSRSSEPHWKDFLGKWRA"
     gene            6663..7643
                     /locus_tag="AC16CX_17990"
     CDS             6663..7643
                     /locus_tag="AC16CX_17990"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014438.1"
                     /translation="MKTIAIEEHFITPLYREKVPANEFRNFYLKARSEQLGHDIVEEN
                     LDLGAKRLAHMDAAGVDVQVLSFGSPGPQPFDPETSIALARDANDRMHEAVKAHPSRF
                     AAFAALPTPDPRAAADELERCVTRLQFKGAMIHGHTRGSFLDERKFWVIFERAEALDV
                     PIYLHPALPHFDAVKAYFDGYEELARAGWGFMIDTSCHFLRILFSGAFDAFPRLKIIL
                     GHLGEGLPFAMHRLQEHTWRAAQRRGLKKTPLEYIRDNLLVTTSGNWYEPAFVCTLLA
                     LGADRILYAIDWPYESNQVGIEWLKKISISDLDREKIAHLNAERILRIAS"
     gene            7657..8622
                     /locus_tag="AC16CX_17995"
     CDS             7657..8622
                     /locus_tag="AC16CX_17995"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014439.1"
                     /translation="MKRFAQRTVLAFALIGLPGIAPAQQFPAGPITVVIPLAPGDAAD
                     VTARSMGEEISRLLKTPVVAINRPGAGGAVGTNIVVQARKDGHTILFAQNSALTFRAV
                     LDPRSVTYDALRDLVPLGIASRSPSILVVRSDVPYRNFAEFIEYSKKNPGQVRVGQPG
                     AGSVGDFCVQLINALTGAGVVSVPYTGAAPAIAALRGGHIEGVVLALGALSTHIKSGA
                     FRGLLASSQYPEFADIPTMRELGYREDLFGVWWSFLAPAGIPEDARKALVSAIEQAVK
                     SPAITARLLPLGILQSYAAPEQTTAEIRDEFKRVSELAKNTGLVK"
     gene            8741..10036
                     /gene="rlmD"
                     /locus_tag="AC16CX_18000"
     CDS             8741..10036
                     /gene="rlmD"
                     /locus_tag="AC16CX_18000"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR3014440.1"
                     /translation="MQVLTVESLDQEGRGVARRDGKAVFVEGALPGEIVACTTQRSKP
                     SYEIAALDRVIEESASRVTPRCPHFGRCGGCSLQHLDARAQVAVKQRVLEDSLWHIGR
                     VRPERILPAIHGPAWGYRHRARLAVRYVPKKGGALVGFHEKRSSYVADMDSCEVLPSR
                     VSALIRPLRDLVNVLSIRNRLPQIEIALGEGADVLVLRVLEPLLPPDVGLLRRFADQR
                     GVRLYLQPGGPETAGPMSATDEADLYYDLPEFDLRIRFGPTDFTQVNHAMNRVLVRRA
                     LALLDPQPGERVADLFCGVGNFTLAIARRGASVVGVEGSPELIRRAAENAEKNGLSRL
                     ASFVDMDLYPPTSDGLAGLGRFDRMLLDPPRDGAVDVVKTLLDDGPLRLVYVSCNPGT
                     LARDAAVLVQTRGYRLSAAGVVNMFPHTSHVESIAVFDR"
     gene            complement(10166..10867)
                     /locus_tag="AC16CX_18005"
     CDS             complement(10166..10867)
                     /locus_tag="AC16CX_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HIR3014441.1"
                     /translation="MQPELQPNQAGYQVGTREIALEQQRVLRNTYLLLGLTMVPTVIG
                     AFIGIGTGGIIMQYPIISFFVMLAAVIGLQFAIVANRNSWVGVALLLAMTFLLGWWLG
                     PLLAVALTLKNGPQLIGVAAAGTGAIFFVMAGIATTVKKDFSFMGKFLFVGMIVALLA
                     LVANMFLQIPALALTISAIIVVVFALFLLHDVSRIVNGGETNYIMATTGCYLSLFNIF
                     ANLLQILMALTGERD"
     gene            11028..11117
                     /locus_tag="AC16CX_18010"
     tRNA            11028..11117
                     /locus_tag="AC16CX_18010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11062..11064,aa:Ser,seq:tga)
     gene            complement(11250..11549)
                     /locus_tag="AC16CX_18015"
     CDS             complement(11250..11549)
                     /locus_tag="AC16CX_18015"
                     /inference="COORDINATES: protein motif:HMM:NF046299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3014442.1"
                     /translation="MKPFERITNPREIRRRLRLNQQEFWSRVGVTQSGGSRYESGRSM
                     PKPVRELLRLVHIEGIELAKAKGNDFEVVSYLKTKNPRLYRRLRKEATRSNGRKK"
     gene            11723..12949
                     /locus_tag="AC16CX_18020"
     CDS             11723..12949
                     /locus_tag="AC16CX_18020"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR3014443.1"
                     /translation="MALIVQKYGGTSVGSTERIKNVARRVARWKAKGHQLVVVVSAMA
                     GETNRLIALAREAQAHPDPRELDVMLSTGEQVTIALLSMALMELGLKARSYTGAQVKI
                     LTDSAYTKARIVSIDEALIRKDLAEDRVVVVAGFQGVDGNGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRIVPEARRLKTVTFEEMLEMASLGSRVLQIRSVEF
                     AGKYQVRLRVLSSFEEESEGTLITFEEDQNMEQASISGIAFNRDEAKITILGVPDRPG
                     IAYQILGPIGDANIDVDMIVQNVSHDGLTDFSFTVHRNDYQKTLKLLEPVVKHIKARG
                     VEGGDKIAKVSVVGVGMRSHAGIASTAFRTLAEEGINIEMISTSEIKISIVINEKYME
                     LAVRALHKAFGLEQPA"
     gene            13181..13273
                     /locus_tag="AC16CX_18025"
     tRNA            13181..13273
                     /locus_tag="AC16CX_18025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13215..13217,aa:Ser,seq:gct)
     gene            13363..14361
                     /locus_tag="AC16CX_18030"
     CDS             13363..14361
                     /locus_tag="AC16CX_18030"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014444.1"
                     /translation="MRRHITLVLVALAALAAAGIAWGQATSTSSGQAYPTRPIRVIIP
                     FPPGGNVDVFARVLYRFVEENLGQTIVIDNRGGANGILGADLVKNATPDGYTYLNTSF
                     GLAVNKFIVKKLPFDLEKDFLPVTKVAVGTGYLMVANPNFQAKTVKELIALAKQQPGK
                     IRYSTAGIGNGQHLAGALFALKAGIDMLHVPYKGGGPAANAVLAGEVQIHYPAGSVGV
                     PHVKAGRMRALAFTGEKRLVSLPDVPTIAEAALPGFVADAGWHGVFAPVKTPMPLATR
                     FQQAVQKALQVPQVRDHFVNNGYEPSGESPAAWAKGYRESLKQLGEIAKAAKIEPQ"
     gene            complement(14410..15330)
                     /locus_tag="AC16CX_18035"
     CDS             complement(14410..15330)
                     /locus_tag="AC16CX_18035"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014445.1"
                     /translation="MPLASLAQSYPSRPLRFIIPFPPGGPTDILGRVVASRLAETWGV
                     PVVADNRPGAGGNIGSEQCARSPADGYTVCMLSIAQTISPSLYSKLGFDPTRDFAHVT
                     LLATLPSLLVVHPSLPVRNVKELVALAKARPGALNYASGGLGTSTQLLMELLKLQAGI
                     RITHVPYKGTGPALVDQISGQIEVAFSTIIAVLPYTRSGRLRAIAVSTRERFPTLPGV
                     PTVAESGLKDFDGGSWQGIVMPAGTPRDAVNKVSLDLMKMLRSPDTKEKILAMGGITY
                     GNSPEEFSAFVRAEVEKWARVVKAAGIRSE"
     gene            complement(15408..16694)
                     /locus_tag="AC16CX_18040"
     CDS             complement(15408..16694)
                     /locus_tag="AC16CX_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3014446.1"
                     /translation="MSSPCELSAAEAARRIARRQLSSEALVAACLERITARERDVQAW
                     AFLDPELALKRARALDREPPRSPLHGVPVGIKDIFDTSDMPTEYGSPIYRSHRPQWDA
                     ACVALLRRAGCVILGKTATTEFAHIHPAKTHNPHNPAHTPGGSSSGSAASVADHMVPL
                     ALGTQTGGSVIRPAAFCGVVGLKPSYNSINRAGLKFAAESLDTIGLFARSAEDVALGL
                     HVLAGRAVPDFEQARGRKPRVGLCRTPRWQDADAATHDAIERVAARLAKSGAQIGDFE
                     LPQGGAQLFEEHGKIMYYETARALAWEYLNHPDQISVTLRPRIEEGWQLPREAYDAAR
                     LLARDCRRRFSDQMNDYDFLVTPSAPGEAPGTLETTGDSVFNRVWTLLGVPCVTLPCD
                     TGPHRLPLGVQLVGRFDGDVELLAWAHWTERVIGSS"
     gene            16812..17780
                     /locus_tag="AC16CX_18045"
     CDS             16812..17780
                     /locus_tag="AC16CX_18045"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014447.1"
                     /translation="MGKLRATFSAALCVLLALLATPAPAQHYPTKPIRVVVAFAPGGI
                     ADTIGRLVGQKLNDRFGQPIVVDNRPGAGGAVAAKLAAGATPDGYTLLVTTTAIAVNA
                     SAVRDAVDPRTQLVPIAIAASAPTIFVVHRSIGAKTLMEYVRTTKKGRFNYATAGVGT
                     TEHLTSEFLFKAVPGLEATHVPYQGGAAPVAAVAGQQVDMATTTTPTAYVFIKQGTLR
                     VLAVASKKRIPVLPDVPTLAEAGFPDFENASWIAFFAPAKTPSAVVRTLNAEVNQALR
                     QPDVRERLTALGFDIQTRSQPEFAEFLKSEVAKWVQVIKVTGISPN"
     gene            17794..18774
                     /locus_tag="AC16CX_18050"
     CDS             17794..18774
                     /locus_tag="AC16CX_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014448.1"
                     /translation="MKTPVSGLCRWFAAAALAVVCGAAAAAQEYPSKPIRFLLPFAPG
                     GIGDLTARVVAQKLGENMGQNVVVDNRPGAGMILSATAALQSAADGYTMILAGNGTAI
                     SATLFKSLPYNILKDFTQVSTLASFDLVLVTTPKSKLKSVADLIATAKQNPGKLNFGT
                     VIVGSTQNLSAELFKSVAGIEAQVVPFKGTPALVAALRADNIDVAFEFAPPAIPHIKA
                     GALKALAVAAAKRHPDMPDVPTTVEAGLADFQVSSWNAVSVRAQTPRAIVERLNREIV
                     TALGSPEVKQKLNALGAEPYPSTPEQTRERMRSEIARWKAVIERANIPRQ"
     gene            18863..19852
                     /locus_tag="AC16CX_18055"
     CDS             18863..19852
                     /locus_tag="AC16CX_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006409881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014449.1"
                     /translation="MPQQSELPLKIAIATYGHTAALKQGKVPIAGVKPEFVEVKPIIA
                     AFRRMVRDVEFDVCEMAPATYMIARAAGSPFKALPVFIYRRFHHAGLVYRDDAGIRKP
                     KDLEGKKVGVRAYSVTTGIWTRGILQNEYGVDISKVTWVVDDEEHVKSLRLPPNVVHA
                     PPGRSLVDMMAAGGLQAAFTDNAGIGRAGPPKDGWQASGQARPPNYVELFPDAPRLEA
                     EWFRRTGIYPVHGLIVVKDALLARHPWVAKALYDAFVESKERYRAQLKSGESVSDKDD
                     FYRQMAAIVGDPLPYGVKANRPAIDALITYCHQQGLLERRFSAEEMFVDPEAA"
     gene            19898..20752
                     /locus_tag="AC16CX_18060"
     CDS             19898..20752
                     /locus_tag="AC16CX_18060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014450.1"
                     /translation="MATKIVDIHPHIISSDTRRYPLSPLGGEQSGWSRKRPVSFEQLI
                     VAMDRAGVDKAAIVHASTVYGYDNSYVADAVAARPDRFTGVFSVDVLAPDAPDKIRYW
                     VGRNLSGLRLFTTGSTMPGQAPWLEDRRLLPAWECAGELNIPICMQMTPAAIPHLEKL
                     LKRYPRIRVILDHLVKPDIEEGPPYAKASFLFDLARYGNVYLKLTSRNVEESRRGKAT
                     PETFFARLVKEFGASRIAWGSNYPASEGTLDQFVTEAKQALASLTQQDRDWILHRTAQ
                     TLYPALAD"
     gene            20975..21871
                     /locus_tag="AC16CX_18065"
     CDS             20975..21871
                     /locus_tag="AC16CX_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR3014451.1"
                     /translation="MSNGEVIKLRALLGDYPNTRAVKQGEVRSPRVAFDFADVKTPNH
                     AFKRVVRDFEFDVAELAIVTYLTAKAHGKPLVLFPAVVRGKFQHESIVCRAERPLAPA
                     DLAGRRVGIRSHSVTTVTWVRGILQNDYGVDLNRVHWVTFEDAHVAEVSDPAAFERAA
                     AGKDPMTMLVAGELDAAVLTGASLKDPRVRPVISDPEAAAKVWYQRYGAVPINHMVVV
                     KESLLKSNAWVAEEVFGLLAAARKTAGPASKVGPDPFPFGVEANRKSLELIIRYAAQQ
                     GLIPRQYEVNELFNDQTRVLRA"
BASE COUNT         4083 a         6941 c         7117 g         3758 t
ORIGIN      
        1 cgccctccgc gcaccgggcg caacccaaaa tcgggggaga aggtacaggt gccggccaaa
       61 tacgtgccgc acttcaaggc cgggaaggaa ttgcgcgaac gagtggatat caagaaataa
      121 gcattcccgg cgttgggtta ccgagggcgg catcatcgaa agatcatgcc gctttttttc
      181 gcacaaggaa tttgtcggtg tcagtcccga caaattcctg acaggaatct caacataaca
      241 actattggat cgttccgaaa aataaagcgt gaagagacgc atattgcgaa gtcttttctt
      301 gtatgtcgta ttaccctaag aacttgttgg gtcgcaggcc gacaagttcc taatggagcg
      361 ggccatgcgt tatctcgtct ggatcctgaa actcgccgtg ttcgtgctgg tcctggcgtt
      421 tgcggtcaag aacaccgggc tggtaacggt gcgctattac ttcggcgggg agtggcaatc
      481 gccgctcgtc ttcgtgctgc tggttgcctt ctgcatcggg gtcgcggctg gtctttccgc
      541 cgggctcgca caggcgttcc ggcagcgccg cgaaatcgcc gcgctcaagc gcgagctgcg
      601 gcagatcaat cgcggaggca cagagggcac ggaggggacg gcagggctga ttcgaggctg
      661 atgacacgtg ttggaaattg agtactggtg gctgctcgcg ctgccgctgt tcttcgcgct
      721 cggctggctt gccgcgcgca tcgacatcaa gcagttgctg ctggaatcgc gcgcgctgcc
      781 catgtcctat ttcaagggcc tcaacttcct gctgaacgag cagccggaca aggcgatcga
      841 ggctttcatc gaggtcgtca aggtcgatcc ccagaccatc gagctccatt tcgcgctggg
      901 aagcctgttt cgccgccgcg gcgaggtcga acgtgcgatc cgcatgcacc agaacctggt
      961 cgagcgcccc gatctcgcgc ccgagcggaa gctcgccgcg ctgttcgagc tcgcgcagga
     1021 ctatttcaag gcggggctcc tcgaccgggc ggaagagctg ttcctcaagc tcgacacgac
     1081 cgcgtatgcc gagcaggcgc tccgcttcct gctcgagatc ttcgagcagg agaaggactg
     1141 gcacaaggcg atcggcatcg ccggcaagct ggaagcggtg acagggcgct cgcaccagaa
     1201 ggaaatcgcc aatttctgct gcgagctggc gatcaacgag atgatgcact cgcggcccga
     1261 cgccgcgcgc ccgcatctcg cgcaggcgct tgcgcaccac cggctgtgcg tgcgcgcgaa
     1321 catgctgctc ggggacctcg aggccgcggc ggggcgcccg gaagcggcga tcgaggactg
     1381 gaagcgcatc gaaagccaga acccccctta tcttgcgctg gtcgcggagc ggctctacaa
     1441 cgcgttcaag cagcgcggca agatcgagga gggcgtcaac ctgctccgcg gctatctgga
     1501 caaatacccc tcgctcgatc tgctcaacgt ggtgttccag gggacgctgg agagccgggg
     1561 cgcagagccg gcgtaccggc tgatgcgcga cgagctgcgg cgcatgccga cgctgctcgg
     1621 gctcgacaag ctgctggaag cgcagctgct ggaagcgccg ctcgatcggc gccgtgatct
     1681 cgagctcgtc aagcagctcg tcaaccagca gacccgcggg ctcgccatgt acaagtgcga
     1741 caactgcggc ttccgggccc gccagtacta ctggcattgc ccggcgtgcg ccgcgtggga
     1801 gacgtactcg ccgcggcgca ccgaggaagc gaggctaccc gcgtgaacga cccgcggatc
     1861 atcgtcgcgc tcgactaccc cggcgccagg gaggcgctcg agctcgccga ccggctcgat
     1921 cccgcgctgt gccgcctcaa ggtcggagcc gagctctaca ctgccgccgg tccccgcttt
     1981 gtcgaacaat tgctgcaatc ggggttcgcc atctttctcg acctcaagta ccacgacatt
     2041 ccgaatacgg tggcgggggc ctgcgtcgcg gcggccgacc tgggggtgtg gatgatcaac
     2101 gtgcacgggc tgggcggacg cgcgatgctg ctggcagcac gcgaggcgct ttccgggcgc
     2161 gcggcgcggc cgaagctgat cgcggtcacg ctgctcacca gcatcggcga agccgaggcg
     2221 acggaaatcg gccttgctgg caatccgcaa gaagccgtcg caaggctggc gcgcctggcg
     2281 caagccagcg ggctggacgg cgtggtgtgc tcgccgcagg aggctgcggc gctgcgccgc
     2341 caattcggca aggagttctg cctggtgacg ccgggcatcc gtcccgcgga gacggcgcag
     2401 gacgatcagc aacgcattgc cacgccgcgc caggcgatcg cgaacggcgc ggattatcta
     2461 gtcatcggca ggccgatcac gcgcgcgccc gatccgctcg cggccttgcg caggatatgc
     2521 gacgaaatag cgcggtgaaa acggcgtcac cgcgctaaaa tgaaaatctg taccaattgc
     2581 gagaaggcgg agtgatgggg atggcagccg gaacaatcaa gccgaagcgc ggtgcttcgg
     2641 cctcatccct catcccccat ccctcatccc tcaagaacgc tcgcgtgctg gtcgtcggcg
     2701 acgccatgct cgaccgctac tgggtcgggg aggtcgagcg tatttcgccc gaagcgccgg
     2761 tacccatcgt cagaatcggt cgggaagagg agagagcggg cggcgcagcg aacgttgcgc
     2821 gcaatgccgc ggcgctgggc gcccaggtaa gcctgctgtc ggtggtgggc gtggacgagc
     2881 ccggcgcccg tctggagagc ctcctcaagc gcgagcgcgt caacgcccaa ctgcaccgcg
     2941 atcccggcat ccggaccacc gtcaagctgc gcgtgatcgc gggacgcaat cagcagctgc
     3001 tgcgcgtgga tttcgagacg ctacccaacc acgaggtgct cgcttccaag ctgcgcgatt
     3061 tcgggcggat gctggctcac gtggacgtcg tgattctgtc cgattacggc aagggcggtc
     3121 tcgcgcacat cgtcaaaatg atcggcatgg cgcgccgcgc cggaaagacg gtgctggtgg
     3181 atcccaaagg tcaggactat tcgcgctatc gaggagccac gctcattacg ccgaaccgcg
     3241 cggagttccg gcaagtggca ggcccctgga aaggcgagcg cgagttgacg gagtgcgccc
     3301 agcggctccg gcgcaaactt gatgcccagg cattgctcgt cacgcgcggc gaggaaggca
     3361 tgacgctgtt ccaggatggc acgcgatttc acgtcgccac gcaggcgcgc gaagtgtccg
     3421 acgtgagcgg cgcgggtgac acggtaatcg cgacgctggg agtggcgctc gccagcgggg
     3481 ctcgcatgcg ggacgcggtg cggcttgcca accgcgccgc ggggatcgtt gtgggcaagt
     3541 tcggcaccgc cgttgtgact cccaaagagc tgtttgggag tcacagtgat aagtgacaag
     3601 tgataagtga taggagttgg tgtaaaacgc gcatgatggt tgcttatcac ctatcaccta
     3661 tcacctatca ccctgagcgg agcgaagcgt gagttacgtt gtcactggag cagcaggatt
     3721 catcggctcc aatctggtga aggcgctcaa cgagcgcggc atcaccgaca tcctcgccgt
     3781 ggacgacttc aaggacggcg gcaagttcat caatcttgcc gattgcgaga tcgccgatta
     3841 tctcgacaag ggtgagttcc tcgcgaagct cgaggatgga gacttcgacg gccagatctc
     3901 cgtcattttc caccagggcg cctgctcgga cactaccgag accgacggcc gctacatgat
     3961 ggagaacaac taccgttact cgcacaaact gctcgactat tgcatcggcg aggaagtgca
     4021 cctcatctac gcgtcgtccg ccgcgatcta cggcgcgagc cgcgagttcc gcgagacgcg
     4081 cgagtgcgag gcgccgctca acgtctacgg ctattccaag ttcctgttcg accagctggt
     4141 gcggcggcgc tacgaggagc gcaccgcgca ggtcgtgggt ctgcgctact tcaacgtcta
     4201 tggcgagcgc gagagccaca aggggcgcat ggcctccgtc gcgcaccact tcttcagcca
     4261 gtaccgcggc accggccacg tgcggctgtt cgccggcagc ggcggctacg gcgacggcga
     4321 gcagcggcgc gatttcgtgt cggtggagga ctgcgtcaag gtgaacctgt tctttctcga
     4381 ccatcccgac cgatcgggca tcttcaacgt cgggaccggc gcggcgcaga gcttccacga
     4441 cgtggcggtc gccaccgtca atgcctgccg caaggcgcgc gccgagacgc cgctcacgct
     4501 caccgcgatg cgcgagcgca gcatcatcca gtacatcccg tttcccgacg acctgcgggg
     4561 ccgttatcag agctataccc aggctgacat ctcggcgctg cgcgccgccg gctacggcgc
     4621 gccgtttctc accgtggaac agggcgtggc gcgctactgc cagaagctgc tcgcgcgggg
     4681 ctgatcaccg gccggcgacc tcgcgcctca agaacccgat cgcagagggg tttttttcgt
     4741 tctgaaaccg gcggctcggt tatcataagc acatggacac acgcccgatc gccggtttca
     4801 gcacgctaga ggacttcgtc ggcaacacgc cgctggtccg cttgaagcgc ctgcccggca
     4861 ggaccagcaa tacgctgctg gcgaagctcg agggcaacaa tcccgccggc tcggtcaagg
     4921 accggccggc cctctccatg atccagcgcg cgcagcagcg cggcgatatc aagcccggcg
     4981 ataccctgat cgagccgacc agcggcaaca ccggcatcgc gctcgccatg tgtgcggcca
     5041 tgatgggcta ccgcatgatc ctggtgatgc cggagcacct gtcgctcgag cggcgccaga
     5101 ccatggcggc gttcggctcc gagatcgtgc tgacgcccaa ggagggcggc atggagatgg
     5161 cgcgcgacgt cgccgagaag atgcgcgacg aaggcaaggg cgtgatcctc gaccagttcg
     5221 cgaatcccga caacccgctc tcgcactacg agggcaccgg gccggagatc tggcgcgata
     5281 cgcagggccg cataacgcat tttgtctcga gcatgggcac gaccggcacc atcatgggct
     5341 gctcgagctt cttcaaggag aagaacccga agatccagat cgtcggttgc cagccctccg
     5401 agggctcgca gattcccggc atccgcaaat ggccgccgca atacctgccg aaaatctgcg
     5461 attggtcgcg cgtggaccgc gtgatcgagg tttcgcaggc cgacgccgag gaaatgacgc
     5521 gccgcctcgc gcgcgaggaa ggcatcttcg ccggcatctc ctccggcggt gcgttgtggg
     5581 cagcgatgca ggtcctgcgc gaggtggaga acgcggttat tgtcacgatc gtgtgcgacc
     5641 gcggcgaccg ctacctgtcg acgggcgtct tccccggctg agaatactga accgccaagg
     5701 ccgccaagag aaacgagaat aagcagaatt aaggcagcaa ggtggtcact gtgtcgccct
     5761 ctgcgtgaat tctgtgttct ctgtggtttg atttctaatg acacccgtcc tcgccttcga
     5821 tatcgagacg gtgcccgatg tcgaagggct gcgccgcctc cacggcctcg acaacagcgt
     5881 tccggacaag gcggtcgcgg agatggcgtt caagctgcgc cggcagacag ccggcacgga
     5941 cttccttccg ctgcacctgc accacgtcgt cgtcatctcc tgcgcactgc gtgaccgcga
     6001 gagcttcaag gtgtggtcca tcggcggcga gagcgagggt gagctgatcc agcgcttctt
     6061 cgacggcatc gaccgctata cgccccagct cgtctcctgg aatggcggtg gcttcgacct
     6121 gcccgtgctg cactatcgtg ggctgatcca ccgcgtaaag gcggcgcgct actgggacat
     6181 gggcgaggac aaccgcgatt tcaagtggaa caactacatc agccgctatc acacccgtca
     6241 ccttgacctc atggacctgc tcgcgcttta tcagccgcgc gccaacgcgc cgctgggcga
     6301 tctcgcgcaa ctcatggggc tgcctggcaa gctcggcatg gaaggcgcag gagtgtggga
     6361 cgcttatcag gcgggcaagc tggctgagat ccgcaactac tgcgaagccg acgtcgtcaa
     6421 cacctatctg gttttcctgc gcctccagct gatgcgcggc gcgctgaccg accagcaata
     6481 caagactgaa agcgatgtag tgcgcgcgat gctatccagg tcgtccgagc cccattggaa
     6541 ggatttcctg ggcaagtggc gggcgtagat cggacccgcg caaatccgga ggcaaggcgc
     6601 cggttcgcca ttgcgctgct catgggccgg cggacgaatc tccacgagaa aggcggcatg
     6661 gcatgaaaac gatcgcgatc gaggagcact tcatcacgcc gctataccgc gagaaggtac
     6721 cggcgaacga attccgcaac ttctacctca aggcccgcag cgaacagctc gggcacgata
     6781 tcgtcgagga gaacctggac ctcggcgcga agcgcctcgc gcacatggac gcggccgggg
     6841 tcgacgtgca ggtactgtcc ttcggttccc ccggcccgca gccgttcgac ccggagacgt
     6901 cgatcgcgct tgcgcgcgac gcgaacgatc gcatgcacga agccgtcaag gcgcatccat
     6961 cgcgctttgc cgcgttcgcc gcgctgccca cgccggatcc gagggcggcc gccgatgagc
     7021 tggagcgctg cgtaaccagg cttcaattca agggcgccat gatccacggg cacacgcgcg
     7081 gcagctttct cgatgagcgg aagttctggg tgatcttcga gcgcgccgag gccctcgatg
     7141 tgccgatcta cctccatccg gcgctgccgc atttcgatgc cgtgaaagcg tatttcgacg
     7201 gttacgagga actggcgcgg gccggatggg gcttcatgat cgacaccagc tgccacttcc
     7261 tgcgcatcct cttctccggc gcgttcgacg cctttccccg gctgaagatc attctcggcc
     7321 atctcggcga ggggcttccg ttcgcgatgc accgcctgca ggagcatacg tggcgcgccg
     7381 cgcaacgccg cggcctgaag aaaactccgc ttgaatacat ccgcgacaat ctgctcgtca
     7441 ccacgagcgg aaactggtac gagccggctt tcgtctgcac gctgctcgcg ctcggcgccg
     7501 acaggattct gtatgcgatc gactggccgt acgagtccaa ccaggtgggc atcgagtggc
     7561 tgaagaagat ctcgatcagt gatctcgacc gcgaaaaaat cgcccatctc aacgccgaac
     7621 gcatcctgcg catcgcatcc tagggaggac ccggccatga aacggttcgc gcagcgcact
     7681 gttctcgcct tcgcgctcat cggtcttccg gggatcgccc cggcccagca gttcccggct
     7741 ggtccgatca ctgtcgtgat tccgctcgcc ccgggcgacg ccgcggacgt caccgcgcgc
     7801 tccatgggcg aagagatttc gaggctgctg aaaacgccgg tagtcgcgat caatcgcccg
     7861 ggcgccgggg gcgcagtcgg cacgaatatc gtcgttcagg ccaggaaaga cggccacacg
     7921 atcctgttcg cccagaacag cgcgctcacc tttcgcgccg ttctcgatcc ccggtccgtc
     7981 acctacgacg cgcttcggga cctggtgccg ctcggcatcg cctcacgctc gccgagcata
     8041 ctcgtcgttc gcagcgacgt gccgtaccgg aacttcgcgg agttcatcga atactcgaag
     8101 aaaaatccgg gccaggtgcg cgtaggccaa cctggcgccg gctcggtcgg tgatttttgc
     8161 gtgcaattga tcaacgcact caccggggcc ggggtcgtgt cggttcccta taccggcgcg
     8221 gcccccgcga tagccgcatt gcgcggagga cacatcgaag gcgtcgtgct ggcgctcggc
     8281 gcgctgagca ctcacatcaa gtcaggggcg ttccgcggcc tcctcgcttc gagccagtat
     8341 cccgagttcg ccgacatacc cacgatgcgc gagctcggct atcgcgaaga cctgttcggc
     8401 gtctggtgga gctttctcgc gccggcaggg atcccggagg acgcgcggaa ggccctcgtc
     8461 agcgcgatcg aacaagcggt aaagtcaccc gccatcacgg cacgactgct gccgctcggc
     8521 attttgcaga gctatgccgc acccgagcag acaacagccg agatccgcga cgagttcaag
     8581 cgggtgagcg agttggctaa gaataccggg ttggtgaaat gacgccgtcg gttctgccgc
     8641 ggaggtggcc tggctatctc agtattatga gctgacggcg ttgcgttaga atcacgctac
     8701 cgctctcgcg gcgcactttg actccgccgc agcgggaaac atgcaggtgc tcacggtcga
     8761 atccttggac caggaaggtc gcggcgtcgc gcgccgcgac ggcaaagccg ttttcgtcga
     8821 aggcgcgctg ccgggcgaga tcgttgcctg cacgacccaa cggagcaagc cgagctacga
     8881 gatcgctgcg ctggacaggg taatcgagga aagcgcatcg cgcgtcacgc cgcgctgtcc
     8941 gcatttcggg cgctgcggcg gctgcagcct tcagcacctg gatgcacgcg cgcaggtcgc
     9001 ggtcaagcag cgcgtgctgg aggacagttt gtggcacatc ggccgggtcc gcccggagag
     9061 aatcctgccc gcgatacacg gcccggcgtg gggctaccgg catcgcgcgc ggctcgccgt
     9121 gcggtacgtg ccgaaaaagg gtggggcact ggtgggattc cacgagaaaa gaagcagcta
     9181 cgtcgcggac atggacagct gcgaggtcct gccgtcgcgc gtgtccgcgc tgatccggcc
     9241 cctccgcgac ctcgtcaacg tgctatcgat ccgcaatcgc ctgccgcaaa tcgagattgc
     9301 gctgggggag ggggcggacg tgctggtgct gcgcgtgctg gaaccgctgt tgccgccaga
     9361 cgtcgggctg ctgcggcggt tcgcggatca acgcggcgtg cggctctatc tacagccggg
     9421 cgggccggaa acggccggcc cgatgtcggc cacggacgag gcggaccttt attacgatct
     9481 gccggagttc gatctcagga tccgcttcgg cccgaccgat tttacccagg tgaatcacgc
     9541 catgaaccgg gtgctggtgc gccgcgcgct cgcgctgctc gatccgcagc ccggcgagcg
     9601 cgtggccgac ctgttttgcg gcgtcggcaa tttcacgctg gcgatagcgc ggcggggggc
     9661 gtccgtggtg ggtgtcgagg gcagtccgga gctgatacgg cgtgccgcgg agaatgccga
     9721 aaagaatggc ttgtcgcgct tggcgagctt tgtcgacatg gatctctatc cccccaccag
     9781 cgacgggctt gcggggctcg gccgattcga ccgcatgctg ctcgatcctc cgcgcgacgg
     9841 ggcggtggat gtggtcaaga cgctgctgga cgacggccca ttacgtcttg tctacgtctc
     9901 ctgcaacccg ggaacgctgg cgcgcgacgc cgctgtcctg gtgcaaaccc gcggctaccg
     9961 cctgagcgcg gcgggggtgg tcaacatgtt cccgcacacc tcgcacgtcg agtcgattgc
    10021 cgtcttcgat cgttagaatc cgacgcaaag acgctaagcc gcaaaggaaa acaaatcaac
    10081 aatcaaaaca aaacggccgg gcatatgccc ggccgtccgt ataaatattg aattggttct
    10141 ctttgcgcct ttgcgtcgag gtttgtcaat cccgctcgcc ggtcagcgcc atcaggatct
    10201 gcagcaggtt ggcgaagatg ttgaacaggc tcaggtagca gcccgtcgtc gccatgatgt
    10261 agttggtctc gccgccgttc acgatgcggc tcacgtcgtg cagcaggaac agcgcgaaga
    10321 cgaccacgat gatcgccgag atcgtcagcg ccagcgccgg gatctgcagg aacatgttgg
    10381 cgacgagcgc cagcagcgcc acgatcatgc cgacgaacag gaacttgccc atgaagctga
    10441 agtccttctt caccgtggtc gcgatgccgg ccatgacgaa gaaaatggca ccggtgccgg
    10501 cggccgccac gccgatcagt tgcggcccgt tcttgagcgt cagcgcgacg gcgagcagcg
    10561 gcccgagcca ccagccgagc aggaaggtca tcgcgagcag cagcgcgacc ccgacccagc
    10621 tgttgcggtt ggcgacgatg gcgaactgaa gcccgatcac ggcggccagc atgacgaaga
    10681 agctgatgat cgggtactgc atgatgatgc cgccggtgcc gatcccgatg aaagcgccga
    10741 tcaccgtcgg caccatggtc agcccgagca gcaggtaggt attgcgcagt acacgctgtt
    10801 gctcgagcgc gatttctcgc gttccgacct ggtatccagc ctggttgggt tgcagttcag
    10861 gttgcattga acgctcctct atagggaaat tttccgctgc gcgtaagact acacaacaag
    10921 gcgcaaagtt tcaatgagtt gggatagttt ttccacgccc cgggcagcgc ggcaactact
    10981 tgatctagcg tgctattatt agccgcgctc atcgtgatcc gaataatgga tgggtggcag
    11041 agcggttgaa tgcacctgac ttgaaatcag gcgtacccgc aagggtaccg ggggttcgaa
    11101 tccctcccca tccgccaaaa aacagcttgg gcagcagccg ggcgcggcct ctgaacggat
    11161 ttcgcgggat atcggcctta ttatgtcggc cgcctgcgcg cctccggggt cgcacccgca
    11221 agcgggcacc tgctcctccc tccggcgcgc tacttttttc tgccgttgct tcttgttgcc
    11281 tctttcctga gacgccgata aagccgcgga ttcttggtct tgaggtagga aacgacttcg
    11341 aagtcgtttc ccttggcctt ggccagctcg atgccttcga tgtgaaccag ccgcaacagc
    11401 tcgcgtaccg gtttgggcat gctgcggccg ctttcgtagc gcgaaccgcc gctctgggtc
    11461 acgccgacgc gggaccagaa ctcctgctgg ttgaggcgca gacggcgccg gatttcgcgt
    11521 gggttggtaa tgcgttcgaa gggtttcatg tcagtcagtc gtcccggttg atatcgcatt
    11581 gaccactgag gacagaatag gttcacgttg gcggctgcgg gcgccggtca tgacgcaagc
    11641 atgaccggca cggccgttgt gccgtctcgt tttcccccga aaaatcaact aaaatacgcg
    11701 actttttccg gttgcagcgg ccatggcctt gatcgtccag aaatacggcg gcacctcggt
    11761 gggctcgacc gagcgcatca agaacgtcgc ccggcgcgtc gcccgctgga aggcgaaggg
    11821 gcaccagctg gtggtggtcg tctccgcgat ggcgggcgag accaaccgcc tgatcgcgct
    11881 cgcgagggag gcgcaggcgc accccgaccc gcgggaactc gacgtgatgc tgtccaccgg
    11941 cgagcaggtg acgatcgccc tgctctcgat ggcgctgatg gagctggggc tcaaagcgcg
    12001 cagctacacc ggcgcgcagg tgaagatcct caccgacagc gcctacacca aggcgcgcat
    12061 cgtgagcata gacgaggcac tgatccggaa ggacctcgcc gaggaccgcg tcgtggtcgt
    12121 cgccggcttc cagggggtgg acgggaatgg caacatcacc acgctcggac gcggcggctc
    12181 ggacacgacg ggggtcgcgc tcgccgcggc gctcaaggcc gacgaatgcc agatttacac
    12241 cgacgtggac ggcgtctata ccaccgaccc ccgcatcgtg ccggaggcgc ggcggttgaa
    12301 gaccgttacc ttcgaggaaa tgctcgagat ggcgagcctc ggctccaggg tgctgcagat
    12361 ccgctcggtc gagttcgcgg ggaagtacca ggtcaggttg cgggtgctct ccagcttcga
    12421 ggaggagagt gaaggcacgc tgatcacttt tgaggaagac cagaacatgg aacaggcaag
    12481 catttcaggc atcgcgttca accgggacga ggccaagatc acgattctgg gcgtgcccga
    12541 ccggccgggc atcgcctatc agatcctggg gccgatcggg gacgccaata tcgatgtgga
    12601 catgatcgtg cagaacgtga gccacgacgg attgacggat ttctcgttca ccgtgcaccg
    12661 caacgactat cagaagacgc tcaagctcct cgagccggtg gtcaagcaca tcaaggcgcg
    12721 cggcgtggag ggcggcgaca agatcgccaa ggtctcggtg gtcggcgtcg gcatgcgctc
    12781 gcacgcgggc atcgccagca ccgcgttccg cacgctggcg gaggagggca tcaacatcga
    12841 aatgatctcg acctcggaga tcaagatctc gatcgtgatc aacgagaagt acatggaact
    12901 cgcggtgcgc gcgctgcaca aagcgttcgg gcttgagcaa ccggcctgaa cggtcagctg
    12961 aaaagacggg agtgcgggag agcgggaaat cgggagatgg aaaaagagtg tgtgttcgat
    13021 ctcgcgccct cacgccctcc cgatctcccg atgtttggta agatggccga gtggtcgcca
    13081 cggcgcagcc gttcgcaaat cctgctcccg gagcggcttc gaccactgga tcgtagaaag
    13141 gtagagcagc agattcttgc agttggaata gtggaaaaac ggagagatgg ccgagtggtc
    13201 gaaggcgctc ccctgctaag ggagtatagg gtcaaaagcc ttatcgaggg ttcgaatccc
    13261 tctctctccg ccaaaaaacg atctgtcgct cgttttttgt aaatctaaaa acaataaaaa
    13321 aacagacggg gacttcccgt ttgttttttt gaggagaaag acatgcgtcg ccacatcacc
    13381 ttagtgctgg ttgctcttgc tgccctggcc gccgcaggaa tcgcctgggg gcaggccacc
    13441 tcgacaagct cgggacaagc ctatcccacc cgcccgatcc gggtcatcat tccgttccca
    13501 ccgggtggca acgtggacgt gttcgcgcgg gttctctacc gcttcgtcga ggagaacctg
    13561 gggcaaacga tcgtgattga caaccggggt ggcgcgaacg gcatcctcgg cgccgacctc
    13621 gtcaagaacg cgacgccgga cggctacacg tatctgaaca cctccttcgg cctcgcggtc
    13681 aacaaattca tcgtcaaaaa gctaccgttc gacctggaga aggatttcct gccggtgacg
    13741 aaagtcgcgg tgggcaccgg ttacctgatg gttgccaatc cgaatttcca ggccaagacg
    13801 gtgaaggagc tgatcgcgct cgcgaagcaa cagccgggaa aaatccgcta cagcaccgcg
    13861 ggaatcggca acggtcagca cctggcgggc gcgctgttcg ccctgaaggc gggaatcgac
    13921 atgctgcacg tgccctacaa gggcggcggg ccggccgcca acgccgtgct ggccggagaa
    13981 gtccagatcc attacccggc gggttcggtc ggcgtgccgc acgtcaaggc gggaaggatg
    14041 cgcgcgctcg ccttcaccgg cgagaagcgc ctggtgtcgc tgccggacgt gccgaccatt
    14101 gccgaggcag cgttgcccgg gttcgtcgcc gacgccggct ggcacggcgt gtttgcaccg
    14161 gtgaagacac cgatgcccct cgcgacgcgg ttccagcagg cggtccagaa ggcgttgcag
    14221 gtgccgcagg tgcgggatca cttcgtcaac aacggttacg aaccgagcgg cgaatctccc
    14281 gcggcttggg ccaagggcta tcgcgagagc ttgaagcagc tcggcgaaat cgccaaggcg
    14341 gcgaagatcg agccgcaata gcagagagtc ctgaatcgtg atttgtgagt cgtgattcga
    14401 cgccgggttc tactcgctgc gaatgccggc ggctttcacc actctcgccc acttttcgac
    14461 ttcggccctg acgaaggcgg agaactcctc gggagagttg ccgtaggtga tcccgcccat
    14521 ggcgaggatc ttctccttcg tgtcgggcga gcgcagcatt ttcatcaggt caaggctgac
    14581 cttgttcacc gcgtcgcgcg gcgtgccggc gggcatcacg attccctgcc acgatccgcc
    14641 gtcgaaatcc ttgaggcccg attcagcgac cgtcggcacg ccgggcagcg tagggaagcg
    14701 ttcccgggtt gacacggcaa tcgcgcgcag cctgcccgac cgggtatagg gaaggacagc
    14761 gataatggtg ctgaatgcga cctcgatctg cccggagatc tggtccacca gcgcgggtcc
    14821 cgtgcccttg tacggcacgt gagtaatccg gatgccggct tgcagcttga gtagttccat
    14881 cagcagttgc gtactcgtgc cgaggccacc ggacgcatag ttgagcgcgc ccggccttgc
    14941 cttggcgaga gcgaccagct ccttgacgtt acgcaccggc agtgacggat gcaccaccag
    15001 caggctcggc agcgtggcga gcagcgtgac gtgcgcgaaa tcgcgggtcg gatcgaaacc
    15061 cagttttgaa tacagcgaag gagagatggt ttgcgcgatg gaaagcatgc agacggtgta
    15121 gccatccgcc ggagatctcg cgcattgctc ggagccgata ttgccaccgg caccgggacg
    15181 gttgtccgcg acaaccggca ctccccaggt ctcggccagc cgtgacgcca ccacgcggcc
    15241 gaggatgtcg gtcgggccgc ccggagggaa cggaatgatg aagcgcaatg gccgggacgg
    15301 gtagctctgc gcaagcgaag ccaacggcac gaacgtcccg gccataaccg agacgagaag
    15361 caagacggta agaaaaaaac gggggcccat tgcaatttgc tccgtaatca cgacgacccg
    15421 atcacgcgtt ccgtccaatg cgcccatgcc agcagttcga catcgccgtc aaaccggccg
    15481 acgagctgca caccgagcgg caaccggtgc gggccggtgt cgcagggcag ggtgacgcac
    15541 ggcacgccca gcaacgtcca cacgcggttg aacaccgagt cgcccgtcgt ttccagcgtg
    15601 ccgggggctt cgccgggtgc gctgggtgtg accaggaaat cgtaatcgtt catttgatcg
    15661 gagaatcgac gcctgcagtc gcgcgccagc agccgcgccg cgtcgtaggc ctcgcgcggc
    15721 agttgccacc cttcctcgat ccggggccgc agggtgacgc tgatctgatc ggggtggttc
    15781 aggtattccc aggcgagggc gcgcgcggtt tcgtagtaca tgatcttgcc gtgctcctcg
    15841 aacagctgcg cgccgccctg cggcagctcg aaatcaccga tctgcgcgcc tgactttgcc
    15901 agccgcgccg ccacgcgctc gatcgcgtcg tgggttgcag cgtccgcgtc ctgccagcgc
    15961 ggcgtccggc acagcccgac tcgcggcttg cgcccccgcg cttgctcgaa gtccggcacc
    16021 gcgcgccccg ccagcacgtg caggccgagc gcaacgtcct cggcgctgcg cgcgaacagg
    16081 ccgatggtgt cgagcgattc cgccgcgaac ttgaggcccg cgcggttgat gctgttgtag
    16141 ctcggcttca ggcccacgac gccgcagaac gccgcagggc ggatcacgga gccgcccgtc
    16201 tgagtgccga gcgcgagcgg caccatgtga tcggcgacag aggctgccga cccgctcgac
    16261 gagccgcccg gggtatgcgc gggattgtgc ggattgtgcg tcttcgccgg atgaatatgg
    16321 gcgaattcgg tggttgccgt ctttccgagg atcacgcagc ccgcgcggcg cagcagcgcg
    16381 acgcacgcgg catcccactg cggccggtgg ctgcgataga tcggcgagcc gtattcggtt
    16441 ggcatgtcgg aggtgtcgaa gatgtccttg atgccgacgg gcacgccgtg cagcgggctg
    16501 cgcggcggct cgcgatcgag cgcgcgcgcc cgcttgagcg cgagttcagg gtccagaaac
    16561 gcccatgcct gcacgtcgcg ctcgcgcgcg gtgatgcgct cgaggcaggc ggcaaccagg
    16621 gcttcgctgc tcaactggcg gcgcgcaatc cggcgcgcag cttccgcggc tgagagttca
    16681 cagggagagg acattccgaa acgcctctga gaaaccggcg cattgtgccc ggtgtggatt
    16741 gtgctggaat agtatcatta tgcacaaccc acttttggcg ggcccatatg caatctggtt
    16801 catttcgatc aatgggtaaa ttgagagcga cattcagtgc tgcgctgtgc gtcctcctcg
    16861 ccttgctcgc gacgcctgca ccggcacagc actatcccac caagccgatc cgggtcgtcg
    16921 tcgcgtttgc gccgggcggc atcgccgaca ccatcgggcg gctggtcggc cagaagctga
    16981 atgaccgctt cggccagccg atcgtggtcg acaaccgtcc cggcgccggc ggagcggtgg
    17041 ccgccaagct cgcggccggg gcaacgccgg atggctacac gctcctggtg accaccacgg
    17101 caatcgcggt caacgccagc gcggtcaggg acgcggtcga cccgcgcacc cagctcgtcc
    17161 cgatcgcgat cgccgcgagc gcgccgacga tcttcgtggt gcaccgttcg atcggcgcga
    17221 aaaccctgat ggaatacgtg cgcacgacca agaaaggacg cttcaattac gcgaccgcgg
    17281 gcgtgggcac gaccgagcat ctgacttccg aatttctgtt caaggccgtg cccggactgg
    17341 aggcgaccca tgtgccgtat cagggcgggg ctgcgccagt cgcggcggtc gccgggcagc
    17401 aggtggatat ggcgacgacg acgaccccga ccgcctatgt attcatcaag caagggacgc
    17461 tgcgggtgct cgcagtggca agcaagaagc gtattccggt gcttccggac gtgccgacgc
    17521 tggcggaggc cggctttccc gatttcgaaa acgcttcctg gatcgctttc ttcgcgccgg
    17581 caaaaacacc gtccgcggtc gtccggacgc tgaatgccga ggtcaaccag gcattgcgcc
    17641 agccggatgt cagggagcgt ctgacggcgc tgggctttga catacagacc cgctcgcagc
    17701 ccgaattcgc cgaattcctc aagagcgagg tcgcgaaatg ggtgcaggtc atcaaggtga
    17761 ccggcatcag cccgaactga taagaggaaa cagatgaaaa cgccggtttc cgggctatgc
    17821 cgctggttcg ccgcggctgc gctcgccgtg gtatgcgggg ccgccgctgc cgcgcaggag
    17881 tatccgtcca agcccatccg cttcctgctg ccgttcgccc ccggcggcat cggcgacctg
    17941 accgcgcgcg tggtcgcgca gaaattgggc gaaaacatgg ggcagaacgt cgtggtggat
    18001 aaccggccgg gcgcaggcat gatcctctct gcgaccgcgg cgttgcagtc ggccgccgac
    18061 ggctacacca tgatcctggc gggcaacggc accgcgatca gcgccacgct gttcaagtcg
    18121 ctgccttaca acatactcaa agactttacg caggtgtcga cgctggcgtc ttttgacctc
    18181 gtgctcgtca cgacgccgaa atcgaaactg aaaagtgtcg ccgatctcat agccaccgcc
    18241 aagcagaatc cggggaaact caactttggc accgtcatcg tcggctccac gcagaacctg
    18301 tcggcggagc tgttcaagtc ggtcgctggc atcgaagcgc aggtcgtgcc gttcaagggg
    18361 acgcccgcgc tggtggcggc gctgcgcgcc gacaatatcg acgtcgcctt cgagttcgcg
    18421 cctccggcta ttcctcacat caaggcgggg gcgctgaagg cgctggctgt cgctgcggcg
    18481 aaacgccacc ccgatatgcc ggatgtcccc accaccgtgg aggccggcct tgcggatttc
    18541 caggtgtctt cgtggaacgc cgtttcggtc cgcgcgcaga cgccgcgggc catcgtggaa
    18601 cggctgaaca gggaaatcgt gactgcgctc ggctcgccgg aggtgaagca gaagctgaac
    18661 gccctgggcg cggagccgta tcccagcacg cccgagcaga cgcgggagcg gatgagatcc
    18721 gagatcgccc ggtggaaagc ggtgatcgag cgcgccaaca ttccgcgaca gtgaatctcg
    18781 ttctcatacc gctctgcggg agactcatca cccatcaccc attacccatc accgaagtca
    18841 gccagcaaca gaagaacatt cgatgccaca acaaagcgag ctgccgctca agatcgccat
    18901 cgccacctac gggcataccg ccgcgctcaa gcaaggcaag gttcccatag caggggtgaa
    18961 gcccgagttc gtcgaggtca aacccatcat cgctgcgttc cggcgcatgg tgcgcgacgt
    19021 cgagttcgac gtgtgcgaaa tggcgcctgc gacctacatg atcgcgcgtg ccgcgggctc
    19081 gccgttcaag gcgctgccgg tgttcatcta ccggcgcttt caccacgcgg ggctggtgta
    19141 ccgcgatgat gcgggcatcc gcaagcccaa ggacctcgag ggcaagaagg tcggcgtgcg
    19201 cgcttactcg gtgaccacag gtatctggac acgcggcata ctgcagaacg agtacggcgt
    19261 ggacatttcc aaggtcacct gggtggtcga cgacgaagag cacgtgaaga gcctgcggct
    19321 gccgcccaac gtcgtgcacg cgccgccggg gcggtcgctg gtcgacatga tggccgccgg
    19381 agggctacaa gccgccttca ccgacaatgc gggcatcggc cgggccggac cgcccaagga
    19441 cggctggcag gccagcggcc aggcgcggcc gcccaactat gtcgagctgt tcccggatgc
    19501 gccgcggctg gaagcggagt ggttccggcg aaccgggatt taccccgtgc acggtctgat
    19561 cgtggtcaag gacgcgttgc ttgccaggca cccgtgggtc gcgaaggcgc tgtacgacgc
    19621 gttcgtcgaa tccaaggaac gctaccgggc gcagctcaag tccggcgaat ccgtgtcgga
    19681 caaggacgat ttctaccgcc agatggccgc catcgtcggc gatccgctgc cttacggcgt
    19741 gaaagccaac cgccccgcga tcgacgcgct catcacgtat tgccaccagc aggggctgct
    19801 ggaacggcga ttttcagcgg aagaaatgtt cgtggatccc gaagcggctt gacgcgcgcg
    19861 ccgccgcgac gacaacgcac acacgaggaa agggctcatg gcaacgaaaa tcgttgacat
    19921 ccacccgcac atcatctcca gtgatacccg gcgctacccg ctctcgcccc tgggcggcga
    19981 gcaatccggc tggtcacgca agcggccggt cagcttcgag caactgatcg ttgcgatgga
    20041 ccgggcgggc gtcgacaagg cggccatcgt gcacgcctcc acggtctacg gctacgacaa
    20101 ttcctacgtg gccgatgcgg tcgccgcgcg gcccgaccgc ttcaccgggg tgttctcggt
    20161 ggacgtgctc gcccccgatg cccccgacaa gatccgctac tgggtgggcc gcaacctgag
    20221 tgggctgcga ctgttcacga ccgggagcac catgcccggg caggcgccgt ggctggagga
    20281 ccggcggttg cttccagcgt gggaatgcgc cggagagctc aacatcccga tctgcatgca
    20341 gatgacgccg gcggcgatcc cgcacctgga gaagctgctc aagcgctatc cgcgcatccg
    20401 cgtgatcctc gaccacctcg tgaagcctga catcgaagag ggtcctccgt acgccaaggc
    20461 gagtttcctg ttcgatctcg cgcgctatgg caacgtgtac ctcaagctca cctcccgcaa
    20521 cgtcgaagaa tcgcgccgcg gcaaggcgac gccggagacg ttcttcgccc gcctcgtaaa
    20581 ggagttcggc gcgtcgcgca tcgcctgggg atcgaattat cccgcttcgg agggcacgct
    20641 cgaccagttc gtaactgaag cgaagcaggc gctggcttcg ctgacacagc aggaccggga
    20701 ctggatcctg caccggacgg cgcaaaccct gtatccggcg ttggcggact gagggaggca
    20761 aggatgaagg cggaaggcgg aaggatgacc tgatctaaat tcccgcactg cgggggtggt
    20821 cagtgacccc ggcccgccgc gctgcatcgc gtcgggcgtt cgccggctcg aaagtgcgca
    20881 ggcactttca tcgaattccc ggcttacccc tcatccttca tccctcatcc ttggcccgtt
    20941 cataaccgta aggccaaatt aggagagata gcacatgagt aacggcgagg tcatcaaact
    21001 gcgggcgctg ctgggcgact acccgaacac ccgcgcggtg aagcaaggcg aggtacgctc
    21061 tccgcgcgtc gccttcgact ttgccgacgt caagaccccc aaccatgcgt tcaagcgcgt
    21121 ggtgcgtgat ttcgagttcg acgtcgcgga gctcgcgatc gtgacttacc tgacggcgaa
    21181 agcccatggc aagccgctcg tcctgtttcc cgccgtcgta cgaggaaagt tccagcacga
    21241 atccatcgtc tgtcgcgccg agagaccgct ggcgcccgcg gaccttgccg gccggcgggt
    21301 tggcatccgc tcacattccg tgacgacggt gacctgggtg cgtggcatac tgcagaacga
    21361 ttacggcgtc gacctcaacc gcgtgcattg ggtgaccttc gaagatgcgc atgtcgcgga
    21421 ggtcagcgac ccggccgcgt tcgagcgcgc tgccgccgga aaagacccga tgaccatgct
    21481 cgtcgcgggc gagctggatg ccgcggtgct caccggggca agcctcaagg acccgcgcgt
    21541 gcggccggtc atatccgatc ccgaggcggc cgcgaaggtc tggtaccagc gctacggggc
    21601 ggtgccgatc aaccacatgg tcgtcgtcaa ggaatcgctg ctgaagtcga acgcgtgggt
    21661 cgcggaggaa gtcttcgggc tgctcgccgc ggcgaggaag accgccggcc cggcctcgaa
    21721 agtcggcccc gacccgttcc cgttcggcgt cgaggcgaac cgcaagagcc tcgagttgat
    21781 catccgctac gcggcgcagc aggggctcat cccccggcag tacgaggtca acgaactttt
    21841 caacgaccag acgcgtgtcc tgcgcgcata acgagcaatg cgggggtcag gtcttgcat
//
LOCUS       DBNGWZ010000576         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1723862, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000576 DBNGWZ010000000
VERSION     DBNGWZ010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1723862"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>2320
                     /locus_tag="AC16CX_18070"
     CDS             29..>2320
                     /locus_tag="AC16CX_18070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014452.1"
                     /translation="MLIATAIVIATAAVTTVEFFTGRVQQALDQQANRLLGADLVIAD
                     SRPLAPELKEDAARRGLSALSIVRFPSMAIRGDRSILADVKVVTPGYPLRGELRVSDR
                     LFVPDRRAGAIPAPGEVWVDERLYTSLELGVGSRITLGNREFRVAAIITDEPGVELGF
                     LSGAPRVMLNAVDLDSTGLVQPGSRVRYRLYIAGGDEALDQYREWARGRLQAGQRMEG
                     IRDARPELRSALERAERFLGLAALASVVLAAVAIALAARRYLQRHLDGCAMMRCLGAG
                     QSLIVRLYVMHFTMLGALSAAIGVGLGAVAQLVLAAWLGEVVAVALPSAGVLPAVQGM
                     LVGLLLLLGFALPPLGSLAHVSTLRVLRRELGIPKGMGALGYAAGAAVIAGMILWQAQ
                     ELRFGFTVLGWFAVAMAAAFALSWLLLRMLGALRARGGSWRFGVASLQRRALGTMLQI
                     VALGLGVMALLTLTVIRTDLLRTWRESLPPDAPNRFIINIQRDQVEPMRGFFNARRLP
                     APEIFPMVRGRLVKIGGRSVSAEDYADERARRLITREFNLSWATRLQADNRVVAGRWW
                     EEGAARADQFSVERGLAEALGISMGDVLTFDVAGAPVAATVTSLRSVEWDSFNVNFFV
                     VAPPGLLEGYPATYVTSFHLVSGRVEFLNALVRAFPNIVLIDIAQALAQVQRMMEQAA
                     RAVQFVFLFTLVAGLAVLYAAVASTQDERIYQATLMRTLGANRRQILRAHLAEFTLIG
                     AVSGFVAAAGATGLAYYIAHRFLH"
BASE COUNT          308 a          784 c          806 g          422 t
ORIGIN      
        1 cacgcgcgat taccgcgccg gggagctcat gctgatcgca acagcgatcg tgatcgcgac
       61 tgcagccgtc accaccgtgg agttctttac cggccgtgtc cagcaggcgc tcgaccagca
      121 ggcaaaccgg ttgctcgggg ccgatctcgt catagccgat tcacgaccgc tcgcgcccga
      181 actcaaggag gacgcggcac gccgcgggct gtcggccctc tccatcgtgc gttttcccag
      241 catggcgata cgcggggatc gcagcatcct ggccgacgtc aaggtcgtca cgcccggcta
      301 tccgctgcgc ggcgagctgc gcgtctcgga ccgcctgttc gtgcccgacc ggcgggcggg
      361 tgcgattccc gccccgggcg aagtctgggt ggatgaacgg ctctatacct cactcgagct
      421 gggcgtcgga agccgcatca cgctgggcaa ccgcgaattc agggtggctg ccatcatcac
      481 cgacgagccg ggagtggagc tgggcttctt gagcggcgcg ccgcgagtca tgctcaacgc
      541 cgtcgatctg gactccaccg ggctggttca gcccggcagc cgcgtccgct atcgcctgta
      601 catcgccggc ggcgacgaag cgctcgacca gtaccgcgag tgggcccggg gacggttgca
      661 agctggccaa cgcatggagg ggatacggga tgcccggccg gaactgcgct ccgcgctcga
      721 gcgcgccgag cgatttctcg gtctcgcggc gctggccagc gtcgtgctgg cggcggtggc
      781 gatcgcgctt gccgcgcggc gctacctgca gcgtcacctc gacggctgcg ccatgatgcg
      841 ctgcctcgga gcaggccagt cattgatcgt ccgcctctac gtcatgcatt tcacgatgct
      901 cggcgcactc tctgccgcga ttggcgtagg gcttggagcc gtggcgcagt tggtgctggc
      961 cgcctggctg ggggaggtcg tggcggtcgc attgccctcc gcgggcgtgt tgccggctgt
     1021 tcagggcatg ctggtcggct tgctgctctt gctgggtttt gccctgccac cgctcgggag
     1081 cctcgcccac gtttcgacac tgcgcgtgct gcggcgggag ctgggtatcc cgaagggcat
     1141 gggcgcgctg ggctacgccg cgggcgccgc ggtcatcgcg ggcatgatcc tgtggcaggc
     1201 gcaggaactg cgtttcggct tcacagtgct cggctggttt gccgtggcga tggctgccgc
     1261 gtttgccttg agctggctgc tcctgcgcat gctcggagca ttgcgcgcgc gcggcgggtc
     1321 ctggcgcttc ggcgtggcga gcctgcaacg ccgggcgctc gggaccatgc tgcagatcgt
     1381 cgctctgggg cttggcgtca tggcgttgct cacgctcacg gtgatccgta ccgacttgct
     1441 ccgcacctgg cgcgaaagcc tgccgccgga cgctcccaac cgcttcatca tcaatatcca
     1501 gcgcgaccag gtcgaaccca tgcgcgggtt cttcaacgca cggaggctgc cggccccgga
     1561 gatctttccg atggtgcgcg gccggctcgt caagatcggc ggccgcagcg tttccgcgga
     1621 ggattacgcc gacgaacgcg cacggcggct tatcacccgc gagttcaacc tgtcatgggc
     1681 gacgcgcctt caggccgaca atcgcgtcgt cgccggtcgc tggtgggagg agggcgccgc
     1741 gcgcgccgat cagttctcgg tcgagcgcgg gctcgccgaa gcgctcggca tcagcatggg
     1801 ggacgtgctg actttcgacg tcgcgggcgc ccccgtggcc gccacggtga cgagcctccg
     1861 cagcgtggag tgggacagct tcaacgtgaa cttcttcgtg gtggctccgc cgggcctcct
     1921 cgaaggctat cccgcgacct acgtcaccag ctttcacctg gtgtccggcc gcgtcgagtt
     1981 tctcaatgcg ctggtacggg cgtttcccaa catcgtgctg atcgatatcg cgcaggcgct
     2041 ggcgcaggtc cagcggatga tggagcaggc ggcgcgcgcc gtgcagttcg ttttcctgtt
     2101 cacgctcgtc gccggcctgg cggtgctcta tgcggccgtg gcgagcacgc aggacgagcg
     2161 gatctaccag gcgaccctca tgcgtacact cggggcgaac cgcaggcaga tcctgcgcgc
     2221 ccacctggcc gagttcacgc tcatcggcgc ggtttcaggc ttcgtggcag ccgccggggc
     2281 gaccgggctc gcgtactaca tcgcgcaccg cttcctgcac
//
LOCUS       DBNGWZ010000577         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2100424, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000577 DBNGWZ010000000
VERSION     DBNGWZ010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2100424"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1360)
                     /locus_tag="AC16CX_18075"
     CDS             complement(119..1360)
                     /locus_tag="AC16CX_18075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3014453.1"
                     /translation="MSAFLSNYAAIAGQDVIRQLRQLCTPLRGMRVVHVNSTREGGGV
                     AEILTKLVPFKRELGIDARWEVITGDEVFFDCTKRFHNALQGVPIDISPALLKAYEAV
                     NAENAERLRPALEEADIVFIHDPQPAALIRHFPARKAKWIWRCHIDLSHPYRSTWNYL
                     RAYVCDYDASIFSLADFAQVLPHPVYLIAPSIDPLSEKNLELDPAEIDAGCAQFHIDR
                     ARPIMLQVSRFDRFKDPLGVIHAYRLVKEFVPSLQLVLAGGGASDDPEGAAVLAEVRS
                     AAEGDPDIHVLQLPADAHRTINALQRAADIVLQKSTREGFGLTVTEAMWKGKPVIGGD
                     TGGIRLQIANHHTGFLVSTPEGAALRARYLLHNRDRLEEMGRKAKQFVRENFLITRFL
                     REHLTLMVGLAREEADRLELG"
     gene            complement(1357..>1912)
                     /locus_tag="AC16CX_18080"
     CDS             complement(1357..>1912)
                     /locus_tag="AC16CX_18080"
                     /inference="COORDINATES: protein motif:HMM:NF039842.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HIR3014454.1"
                     /translation="HAGCIDYHFWGGLLRPSFDDPEYANDFAAWARHGLHDSKLAERL
                     GMIDPAEFADIEALRRELIDVLEERIDEGEYLTWAKPDQQFQFIRSRIVVFDTGRTLA
                     QPSELVAAIPAMSAGSVYYHFVDARRRPPLRIDDFRAWLSRYDAEYAALGDALAEVEP
                     YFTTLTRLREELARAFRASFGGRS"
BASE COUNT          316 a          642 c          638 g          316 t
ORIGIN      
        1 gatcctgaac ggcgcgagcg ttccgtcgta gtcgagcatc agcacgcggc tgtgggccgc
       61 ggcgagccgg tcgtagaagg ccgccaggtc gacgcgtgga ctcagtacct gcatgccctc
      121 acccgagctc gaggcgatcc gcttcttccc gggcgagccc gaccatcagc gtgaggtgct
      181 cgcgcagaaa gcgtgtgatc aggaagttct cgcgcacgaa ttgcttcgcc ttgcgtccca
      241 tctcctcgag gcggtcgcgg ttgtgcagga ggtagcgcgc gcgcaacgcc gccccctccg
      301 gcgtgctcac caggaagccg gtgtgatggt tggcgatctg cagccggatg ccgccggtat
      361 ccccgccgat cacgggcttg cccttccaca tcgcctcggt cacggtgagc ccgaagccct
      421 cgcgggtcga tttctgcagc acgatatcag cggcgcgttg cagcgcgttg atcgtgcgat
      481 gggcgtccgc cggcagctgc agcacgtgaa tgtccgggtc tccttccgcc gcgctgcgca
      541 cttccgccag caccgcggcg ccctccgggt cgtcgcttgc gccgcccccc gcgagcacca
      601 gctgcaacga gggaacgaat tccttgacca ggcggtaggc atggatcacc ccgagcgggt
      661 ccttgaagcg gtcgaagcgc gagacctgca gcatgatggg ccgggcacgg tcgatgtgga
      721 actgcgcgca tcccgcgtcg atctccgccg ggtcgagctc caggttcttc tcgctcagcg
      781 gatcgatgct cggcgcgatg agatagaccg ggtgcggcag cacctgcgcg aaatcggcga
      841 gcgagaagat gctcgcatcg taatcgcaga cgtatgcgcg caggtaattc caggtgctgc
      901 gataaggatg gctcaaatcg atgtggcagc gccatatcca cttcgccttg cgggcgggga
      961 agtgccggat cagcgccgcc ggctggggat cgtggatgaa cacgatgtcg gcctcctcga
     1021 gcgcgggccg cagccgttcc gcgttctccg cgttcaccgc ctcgtaggcc ttgagcagcg
     1081 ccggggagat gtcgatgggc acgccctgca gcgcgttgtg gaagcgcttg gtgcagtcga
     1141 agaacacctc gtcgccggtg atcacctccc agcgcgcgtc gatgcccagt tcccgcttga
     1201 acggcacgag cttggtgagg atctcggcga ccccgccgcc ctcgcgcgtc gaattgacat
     1261 gcacgacgcg catgccgcgc aagggcgtgc acagctggcg caactggcgg atgacgtctt
     1321 ggcccgcgat ggccgcgtag ttgctaagga acgcgctcat gaccgcccgc cgaatgacgc
     1381 tcggaacgcg cgcgcaagct cttcccgcaa ccgggtcagc gtcgtgaaat acggttccac
     1441 ctccgcgagg gcgtcgccca atgcggcata ctccgcgtca taacgggaca gccaggcgcg
     1501 aaagtcgtcg atgcgcagcg gcggccgccg gcgggcgtcg acgaaatggt aatagacgct
     1561 gcccgccgac atcgccggaa tggccgcaac cagctcggac ggttgcgcaa gggtccgtcc
     1621 cgtatcgaac accacgatcc gggagcggat gaactggaac tgctgatcgg gcttggccca
     1681 ggtcagatac tcgccctcgt cgatgcgctc ctccagcacg tcgatcagct cgcgccgcag
     1741 cgcctcgatg tcggcgaact ccgccgggtc gatcatgccg agccgctcgg cgagcttgct
     1801 gtcgtgcagc ccgtggcgcg cccacgcggc gaagtcgttg gcgtactccg gatcgtcgaa
     1861 actgggccgc agcagcccgc cccagaagtg atagtcgatg cacccggcat gc
//
LOCUS       DBNGWZ010000578         6030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2728032, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000578 DBNGWZ010000000
VERSION     DBNGWZ010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2728032"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC16CX_18085"
     CDS             <1..422
                     /locus_tag="AC16CX_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3014455.1"
                     /translation="DRVGPHGRVIALDLLEMAPLRGVTFIRGDFRDEAAVAELERALG
                     GHMADLVLSDMAPNISGIAAADQARALELAELALAFALKHLKPQGNFLVKTFQGTGFE
                     DFLKTLRDRFTAVAVRKPEASRSRSSEVYLVGKGLRA"
     gene            508..2397
                     /gene="ftsH"
                     /locus_tag="AC16CX_18090"
     CDS             508..2397
                     /gene="ftsH"
                     /locus_tag="AC16CX_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3014456.1"
                     /translation="MNNLVKNVAIWLVIALVLMTVFNQFSTRQTVQKAMEYSQFIEEV
                     KQGRIAKVTIEGRVLKGVKTTGERFTTYSPSDPWLVSDLLKNGVIIEAKPEEEPSLLM
                     NIFVSWFPMLLLIGVWIFFMRQMQGGGRGGAFSFGKSRARMLDESNNTITFADVAGCE
                     EAKEEVAELVEFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKHAPCIVFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFETNSGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILQ
                     VHMRKVPIAPDVKADIIARGTPGFSGADLANLVNEAALFAARANKRLVDMDDFERAKD
                     KIVMGAERRSIVMPEHERRNTAYHESGHAVVAKLMPKTDPVHKVTIIPRGRALGVTMQ
                     LPEQDRYSMDREMILQNIAVLFGGRIAEEIFMGQMTTGASNDFERATDLARSMVTRWG
                     MSDELGPMIYGENEGEVFLGRSVTVHKNVSETTMQKVDAEIRRIVDQQYALARKLIED
                     NRDKVEAMARALLEWETLDSDQINDIMEGHAPRPPKPTPAPSQPRDPGSQPTATAPAA
                     PPQGA"
     gene            2483..3316
                     /gene="folP"
                     /locus_tag="AC16CX_18095"
     CDS             2483..3316
                     /gene="folP"
                     /locus_tag="AC16CX_18095"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3014457.1"
                     /translation="MSQTLRCGKFTFDLARPLIMGVVNVTPDSFSDGGLFSDATSAAA
                     HARRLIGEGADILDIGGESTRPGSAAVVLDEERRRVLPVIEALSACGVPVSVDTRKPE
                     LMREAVAAGAAMVNDVTALSGPGALETVAQADCAICLMHMQGDPSTMQVNPSYRDVVR
                     EVRDYLAGRVAAAETAGIARERIVVDPGFGFGKTLEHNLALLRALAEFRSLGVALMVG
                     LSRKAMLGRLTGREPLERIHASVAAALLAVQNGAQIVRVHDVAATRDALTVWRAVMGN
                     E"
     gene            3386..4735
                     /gene="glmM"
                     /locus_tag="AC16CX_18100"
     CDS             3386..4735
                     /gene="glmM"
                     /locus_tag="AC16CX_18100"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004542480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3014458.1"
                     /translation="MTRRYFGTDGVRGKVGREPITPDFVMRLGYAAGKVLAGIRRRGL
                     ERPAVLIGKDTRVSGYMLESALEAGLSAAGVDVLLVGPMPTPAVAYLTRALRLSAGIV
                     ISASHNPYDDNGIKFFSGDGAKLPDATETAIERELARPLKCMTSSRLGKARRIDDAAG
                     RYIEFCKTTFPTSLHLRGMRIVVDCANGAAYHTAPHVFHELGADVETVGVSPDGFNIN
                     EGVGATSPQALQTAVKRQKADLGIALDGDGDRLVMADGTGRLYDGDQLLYVIARHRKQ
                     KSELRGGIVGTQMTNLALEHALARLKIPFARAKVGDRYVLEMLLQRHWQLGGENSGHI
                     VCLDKHTTGDGIISALQVLRALTETGTTLSKTAGALELYPQVLVNVAVKKALDPISSP
                     AVKRAVREAETELDGSGRVLLRPSGTEPVIRVMVEGRDGGTARRLADHIATAVRKSF"
     gene            4792..5157
                     /locus_tag="AC16CX_18105"
     CDS             4792..5157
                     /locus_tag="AC16CX_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009693516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3014459.1"
                     /translation="METKTVVQAFGALAQETRLGIYRLLVASGPEGMAAGAIAGKLDL
                     PPATLSFHLKELSLAGLVRSRQEGRFVIYAANFDTMTGLVAFLTENCCGGRSCAPSVR
                     PIAMKERGHETLPRARRRA"
     gene            5173..5661
                     /locus_tag="AC16CX_18110"
     CDS             5173..5661
                     /locus_tag="AC16CX_18110"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR3014460.1"
                     /translation="MKPYNVLFLCTGNSARSILAEACLNSAGRGRFRAYSAGSHPTGK
                     VNPLALELLAKNRINAPGLRSKSWDEFAHPGAPEIDMIFTVCDNAAGEACPVWPGKPI
                     TAHWGVADPAAVVGSDAEKRKAFQRAFAELSARINVLLALPIEKLDRHALKSRLDEIG
                     RK"
     gene            5658..>6030
                     /locus_tag="AC16CX_18115"
     CDS             5658..>6030
                     /locus_tag="AC16CX_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HIR3014461.1"
                     /translation="MSAPAVAAALGESKPAIGFFERWLTLWVFLCIVVGIALGELMPG
                     PIQALGGLNLARVNIPVGFLIWVMIVPMLMKIDFRALHEVRRHWRGMAVTVAVNWAVK
                     PFSMALLGWIFIRHVFAPWLPP"
BASE COUNT         1082 a         1908 c         2028 g         1012 t
ORIGIN      
        1 cggaccgggt cggaccgcac ggcagggtga tcgcgctcga cctcctcgag atggcacccc
       61 tccgcggagt caccttcatc cgtggcgatt tccgtgacga agccgcggtg gcggagctgg
      121 aacgcgcgct cggcggccac atggcggatc ttgtgctctc cgacatggcc cccaacataa
      181 gtggtatcgc cgcagccgat caggcgcgcg cgctggaact tgcggagctg gcgctggcct
      241 ttgcattgaa gcacttgaaa ccgcaaggga atttcctggt caaaacgttt caggggacag
      301 gcttcgagga ttttctgaaa acgcttcgcg accgtttcac ggcggttgcc gtccgcaagc
      361 ccgaggcttc ccgcagccgt tcgagcgagg tgtacctggt cggtaagggc ttgcgggcgt
      421 agcgttctgt ccaaggacac ggcagatttg ctgcactgcg ggcggaaaaa gtctagaatt
      481 gagttggtta cgagatgagg aacttgcttg aataacctgg ttaaaaatgt ggcgatctgg
      541 ctggtgatcg cgctggtgct gatgacggtg ttcaaccagt tcagtacgcg ccagaccgtg
      601 cagaaggcga tggagtactc ccagttcatc gaggaggtga agcagggacg catcgccaag
      661 gtgacgatcg aagggcgcgt gctgaagggc gtgaagacga ccggcgagcg cttcaccacc
      721 tattcaccgt ccgacccgtg gctggtgtcg gacctgctca agaacggcgt catcatcgaa
      781 gccaagcccg aggaggagcc ctcgctcctc atgaacatct tcgtgtcctg gttcccgatg
      841 ctgctgctga tcggcgtgtg gatctttttc atgcgccaga tgcagggcgg gggccgcggc
      901 ggcgcgttct cattcggcaa gagccgcgcg cggatgctcg acgagtcgaa caacaccatc
      961 accttcgccg acgtcgcggg ctgcgaggag gcgaaggaag aggtcgcgga gctggtcgag
     1021 ttcctgcgcg acccctcgaa gttccagaag ctcggcggac gcatcccgag gggcgtgctg
     1081 atggtcggca accccggcac cgggaagacg ctgctcgcca aggcgatcgc gggcgaggcg
     1141 aaggtgccgt tcttctcgat ctccggctcc gacttcgtcg agatgttcgt cggggtcggc
     1201 gcggcgcgtg tgcgcgacat gttcgagcag gcgaagaaac acgccccctg catcgtgttc
     1261 attgacgaaa tcgacgccgt cgggcgccag cgcggcgcgg gcctgggcgg cggcaacgac
     1321 gagcgcgagc agacgctgaa ccagctgctc gtcgagatgg acggcttcga gaccaactcg
     1381 ggcgtgatcg tcatcgccgc gaccaaccgt cccgacgtgc ttgacccggc gctgctgcgg
     1441 cccggccgct tcgaccggca ggtagtggtg ccgctgcccg acatccgcgg gcgcgagcaa
     1501 atcctgcagg tgcacatgcg caaggtcccg atcgcgccgg acgtcaaggc cgacatcatc
     1561 gcgcgcggca cgccgggctt ctcgggcgcc gacctggcga acctggtgaa cgaggcggcg
     1621 ctgttcgcag cgcgcgctaa caagcggctc gtggacatgg acgatttcga acgcgccaag
     1681 gacaagatcg tcatgggggc ggagcgtcgc tccatcgtca tgcccgagca cgagcgcagg
     1741 aacaccgcct accacgagtc ggggcacgcg gtggtcgcga agctgatgcc caagaccgat
     1801 cctgtgcaca aggtgacgat catcccgcgc gggcgcgccc tgggcgtgac catgcaactg
     1861 cccgagcagg accgctacag catggaccgc gagatgatcc tgcagaacat cgcggtgctg
     1921 ttcggcggac gcatcgccga ggaaatcttc atgggccaga tgaccaccgg ggcctcgaac
     1981 gacttcgaac gcgccaccga tctcgcgcgc agcatggtga cgcgctgggg catgagcgac
     2041 gagctcgggc cgatgatcta cggcgagaac gagggcgagg tgttcctcgg ccgctcggtg
     2101 acggtgcaca agaacgtctc cgagaccacg atgcagaagg tggacgccga gatccgtcgc
     2161 atcgtggatc agcagtacgc gctggcgaga aagctcatcg aggacaaccg cgacaaggtc
     2221 gaggcgatgg ccagggcgct gctcgagtgg gaaacgctcg actccgacca gatcaacgac
     2281 atcatggaag gccatgcgcc gcgcccgccc aagcccacgc cggccccgtc ccaacccagg
     2341 gatcccggct cgcagccgac ggccaccgcg cccgccgcgc ccccgcaagg cgcctgacac
     2401 cccgaaggat gagggatgag ggatgacccc cgcagtgcgg gtttgaaatc aggtcatcct
     2461 tccgccttcc gccctcatcc ttatgtctca gacactccgc tgcggtaaat tcacgttcga
     2521 cctggcacgc ccgctgatca tgggcgtggt caacgtcacg ccggactcct tctccgatgg
     2581 cgggctgttc tccgacgcga catcggccgc ggcgcacgcg cgccggctga tcggggaggg
     2641 cgcggacatc ctcgacatcg gcggggagtc cacgcgtccc ggatcggcgg cggtcgtgct
     2701 ggatgaggag cgacggcgcg tcctgcccgt gatcgaagcg ctctccgctt gcggcgtgcc
     2761 ggtctcggtg gatacccgca agcccgagct gatgcgcgag gcggtcgcgg caggcgcggc
     2821 gatggtgaac gacgtcacag cgttgtccgg ccccggagca ctcgaaacgg tggcgcaggc
     2881 agactgcgcc atctgcctca tgcacatgca gggcgacccc agcaccatgc aggtcaatcc
     2941 cagctatcgg gacgtcgtgc gcgaggtgcg cgactacctc gcaggacggg ttgctgcggc
     3001 ggaaacggcc ggcatcgcgc gcgagcgcat cgtcgtggat cccggtttcg gcttcggcaa
     3061 gaccctcgag cacaacctgg cattgctacg cgcgcttgcc gagttcagat cgttgggcgt
     3121 tgcgctgatg gtggggctgt cgcgcaaggc catgctgggc aggctgacgg gccgggagcc
     3181 gctggaacgc atccatgcga gcgtcgcggc ggcgctgctc gcggtgcaga acggcgccca
     3241 gatcgtgcgg gtgcacgatg tcgccgccac ccgcgacgcc cttactgtct ggcgggcggt
     3301 aatgggtaat gagtaatggg taatgggctc aaaaccgctg taacattcat gtcaaagaag
     3361 gccgttcacc catcacccat cacgcatgac aagaagatat ttcggcacgg acggcgtgcg
     3421 cggcaaggtc ggccgggagc cgatcacccc cgatttcgtc atgcggctgg gctatgccgc
     3481 gggcaaggtg ctggcgggca tccggcgcag gggcctcgag cgccccgccg tgctgatcgg
     3541 caaggacacc cgggtttccg gttacatgct ggagtcggcg cttgaagcgg gactttcggc
     3601 ggccggcgtg gacgtgctgc tggtgggacc gatgcccacg cccgcggtcg cctatctcac
     3661 gcgggcgttg aggctctccg ccggcatcgt gatcagcgcg tcgcacaatc cctatgacga
     3721 taacggcatc aagttcttct cgggggacgg cgcgaagctg cccgatgcga ccgagaccgc
     3781 gatcgagcgc gagctcgcac ggccgctgaa gtgcatgacc tcgagccggc tcggcaaggc
     3841 gcgtcgtatc gacgacgccg cgggccgcta catcgaattc tgcaagacga cgtttccgac
     3901 cagcctgcac ctgcgcggca tgaggatcgt cgtggactgc gcgaacggcg ccgcgtatca
     3961 caccgccccc cacgtcttcc acgagctcgg cgccgacgtc gagacggtgg gcgtgagccc
     4021 ggacggcttc aacatcaacg agggggtcgg cgcgacttcg ccccaggcac tgcagacggc
     4081 ggtgaagcgc cagaaggccg atctcggtat cgcgctcgat ggtgacggcg accgcctcgt
     4141 catggcggac ggcactggcc ggctctacga cggcgaccag ctgctctacg tcatcgcgcg
     4201 ccatcgcaag cagaagagcg agctgcgcgg cgggatcgtc ggcacccaga tgaccaatct
     4261 cgcactcgag cacgcgctcg cccggctcaa gatcccgttc gcccgcgcga aggtgggcga
     4321 ccgctatgtg ctggaaatgc tgttgcagcg ccactggcag ctgggcgggg agaactccgg
     4381 ccacatcgtc tgcctggaca agcacaccac gggtgacggc atcatctccg cgctccaggt
     4441 actgcgcgcg ctgaccgaga ccgggacgac gctctcgaaa accgccggcg cgctcgagct
     4501 ctacccgcaa gtcctggtga acgttgcggt gaagaaggcg cttgatccga tttccagccc
     4561 cgcggtcaag cgggcggtga gggaagccga gaccgagctc gacggcagcg ggcgggtgct
     4621 gcttcgcccg tccggcaccg agccggtgat ccgcgtcatg gtggaaggcc gtgacggtgg
     4681 cacggcacgg cgcctggcgg accatatcgc cacggcggtg cgcaagtcct tctgaaccca
     4741 tcgttgattc gccgcttgac gcgcctctta tatttccata gtattggaaa tatggaaacg
     4801 aaaaccgtcg tgcaggcatt cggggcactg gcgcaggaga cacgtctcgg catctatcgt
     4861 ctgctggttg cgtccggtcc ggagggcatg gccgcggggg cgatcgccgg gaaactggac
     4921 ttgcctcctg cgacgctttc atttcatttg aaggagctga gcctcgccgg cctggtgcgc
     4981 tcgcgacagg aaggccgctt cgtcatctac gcagccaact tcgacaccat gaccggattg
     5041 gtcgcgttcc tcaccgagaa ttgctgcggc ggccgatcct gcgctccatc tgtcagaccg
     5101 attgcgatga aagaaagggg ccatgaaacg cttccacgcg cacgtcgccg tgcgtgacct
     5161 tacctgctgc ggatgaagcc gtacaacgta ctttttctct gcacgggaaa ctcggcgaga
     5221 tcgatcctgg ccgaggcttg cctgaactcc gcggggcgcg ggcggttcag ggcttacagc
     5281 gccggcagcc atccgaccgg gaaggtgaat ccgttggcgc ttgagctact ggccaagaac
     5341 cgcatcaatg ccccgggcct acgctccaag agctgggacg agttcgccca tcccggcgca
     5401 ccggagatcg acatgatctt caccgtgtgt gacaacgcgg ctggcgaagc ctgcccggtg
     5461 tggccgggga agccaatcac cgcacattgg ggcgtagcgg accctgcagc agtcgtcgga
     5521 agcgacgcag aaaagcgtaa agcgttccaa cgtgcgttcg cggaactctc agcccgaatc
     5581 aacgttcttc tcgcgctccc tatcgagaaa ctcgaccggc acgccctgaa gagcagactc
     5641 gacgagatcg gcagaaaatg agcgctcctg cagtcgccgc cgccctgggc gaatcgaagc
     5701 ctgcaatcgg ctttttcgag cgctggctga cgctctgggt attcctttgc atcgtggtgg
     5761 gcattgcgct cggtgagctg atgccgggac cgatccaagc gctcggcggg ctcaatctgg
     5821 cgcgggtgaa cattccggtg ggctttctca tctgggtgat gatcgtcccg atgctgatga
     5881 agatcgactt tcgtgccctg cacgaggtga ggcggcactg gcgcggaatg gcggtgacgg
     5941 tcgcggtgaa ctgggcggtg aaaccttttt cgatggcgct actcggctgg atcttcatcc
     6001 gtcacgtgtt cgcgccttgg ctgccgccgg
//
LOCUS       DBNGWZ010000579         8452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1345958, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000579 DBNGWZ010000000
VERSION     DBNGWZ010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1345958"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16CX_18120"
     CDS             complement(<1..586)
                     /locus_tag="AC16CX_18120"
                     /inference="COORDINATES: protein motif:HMM:NF017519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR3014462.1"
                     /translation="MQAFPGYLGHALAAYLTRPSKSYQPLATNPPGQLRQALRAGDVL
                     LVEGNTRISVAIKYLTQSTWSHAALFVGRRLAHSKDDPDPPVLIEADLVGGVRAVPLS
                     NYDQCHTRICRPVGLAETDCERIIEAAVARIGHTYDLRNVFDLARYLIPTPPVPQRWR
                     RRMLALGSGEPTRAICSTLIAQLFQSVGYPILPRI"
     gene            complement(605..1768)
                     /locus_tag="AC16CX_18125"
     CDS             complement(605..1768)
                     /locus_tag="AC16CX_18125"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR3014463.1"
                     /translation="MRARDAKCTVEGTRWSIAIAALLTWSVAMPADTRLTSEWPRTDF
                     SKRIVSLEEIHSGGPPKDGIPAIDRPQFVTPEEAGKWLDRREPVIVFERNGDARAYPL
                     QILMFHEIVNDIVGSIPVVVTFCPLCNASLVFDRRIAGVVLDFGTTGKLRKSDLVMYD
                     RQTESWWQQFTGKGIVGRHAGTALEQLPSTIVPFSDFRKAYPQGRVLSHQTGFSRPYG
                     KNPYRGYDRVGDIPFLFHDPVDPRLPAMERVLGVAISGKVRVYPFAALAGIRVVNDEV
                     GREPVAIFTSEGMLSALDAELIRDSRRIPAAAAYSRRLGDRTLVFELRGERVFDRDTG
                     SEWDIFGKAVAGPLKGERLKPVDSGVHFAFAWLAFNPQSEIFSPRVAPARDRR"
     gene            complement(1774..2964)
                     /locus_tag="AC16CX_18130"
     CDS             complement(1774..2964)
                     /locus_tag="AC16CX_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HIR3014464.1"
                     /translation="MSYSKISCVTPPVVEAAGAVQLRIQYAWTAATLGLAGLLLFALV
                     GQGWRMPALLLLGMALGAVLLLTSFGFTSAYREFLLYRDTAGVRAQVLMLALATVLFV
                     PVLAAGSAFGQPVSGAFAPVGAQVVAGAFMFGIGMQLAGGCGSGALFAAGGGSARMLI
                     VLAFFVAGSFWASLHMSWWERLPSRGEAALGDLLGWTEALALQLGALAVIWVWLGRVA
                     PGRAERSSRLDGASVRERTLLLGAVLLAVLAFLVLLVAGHPWSITWAFALWGAKTALS
                     FGWQPDESAFWNGDFQHRALESSLFEDVTSVMDIGMVLGAAAGAAGRFRPRLRLSGAS
                     LAAAVIGGLAMGYGARIAYGCNIGAFFSGVASTSLHGWVWIAAALGGNAIGVRLRPWF
                     GLRN"
     gene            complement(2921..3301)
                     /locus_tag="AC16CX_18135"
     CDS             complement(2921..3301)
                     /locus_tag="AC16CX_18135"
                     /inference="COORDINATES: protein motif:HMM:NF015581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR3014465.1"
                     /translation="MTIKTRHGFDAMLTRIEKAIGDHKMGLVAQASASRGAATRGVKI
                     PGNAVLMVFRNDYAMRMLKASVPAGIEAPLRIYVTENADGSASLTYRRPSTVFAPYSS
                     SALDEMAKELDVIFENIVRDAAGG"
     gene            complement(3452..3925)
                     /locus_tag="AC16CX_18140"
     CDS             complement(3452..3925)
                     /locus_tag="AC16CX_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014466.1"
                     /translation="MVKRFPSCRGSFCCLGLSALVLGLPAAPAAAAQIIVLTQLPCQF
                     LESEGVERGYKSRSIKDCEAINAKTAQQRVAQAKPLQLKPGKYVFRVTNKNVPYELGF
                     WLRGASAAGRVTLPSVSGGGLMPGKTRDYEIELKPGEYVYSCPLNTTPDYKLIVR"
     gene            complement(3962..4603)
                     /locus_tag="AC16CX_18145"
     CDS             complement(3962..4603)
                     /locus_tag="AC16CX_18145"
                     /inference="COORDINATES: protein motif:HMM:NF046632.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6969 family protein"
                     /protein_id="HIR3014467.1"
                     /translation="MAGAEVLECQRVLSHGGINLVSEVLRDQGEFVEYEHYPRSDVYD
                     SESGAQYYYHAHRGGEHGHFHTFLRVSTGNRDGWPAGNGPDSDEPVHLIAISMDAYGW
                     PVGLFATNHWVCGGAWLPAEEAIGLLPRFRIDHAYPSWPVNRWIGAMMVLFRPYIEAL
                     LIHRDAVIEAWTRTHPERDAFEDRELEVTGFLPISVEETVREIRALAGIRNPV"
     gene            complement(4840..5334)
                     /locus_tag="AC16CX_18150"
     CDS             complement(4840..5334)
                     /locus_tag="AC16CX_18150"
                     /inference="COORDINATES: protein motif:HMM:NF044772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014468.1"
                     /translation="MTRPKGTKVYKADRATLVKEGEVLWKDTKLSTNGMACNTCHVNN
                     GAFNATFAKPYPHHVAMTQEKGGFKRVSAEEMVQFCMVVPMAAKPLKWESKELAALTA
                     YTLDVQKKFKPNPCAAKNPCAAKSPCGARANPCAAKRNPCAAKNPCAAKNPCGAKNPC
                     GAKR"
     gene            5990..6253
                     /locus_tag="AC16CX_18155"
     CDS             5990..6253
                     /locus_tag="AC16CX_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014469.1"
                     /translation="MAQSVKPDPRLAPLPVEATPELKKTFQTYHKTLGYVPNSALIMQ
                     RKPKLVKAFTALTAAVWDPESEVDRGFKRLIAHVASRTHGCHY"
     gene            6254..6604
                     /locus_tag="AC16CX_18160"
     CDS             6254..6604
                     /locus_tag="AC16CX_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014470.1"
                     /translation="MAHTAGGSLRFGIEEQKLAAVWEYRASPMYTEAERVALDFTIAA
                     ASQPNDVTDELFARLKQHWTENQIVEIVATIALFGFLNRWNDTMATPLEREPIEVAEK
                     FLAPHGWNIGKHHR"
     gene            6691..7116
                     /locus_tag="AC16CX_18165"
     CDS             6691..7116
                     /locus_tag="AC16CX_18165"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HIR3014471.1"
                     /translation="MDAHRAEMTIGKVARKAGLAIDTVRYYEREGLIEKPARSASGYR
                     NYRPDVIARLRFIRQAKNLGFSLSEVRELLALKVAPGKSCADVRARTERKIADVEQRI
                     AQLERMKRALAKLAAACSGRGPTSECPILEAMETHEISQ"
     gene            7113..7328
                     /locus_tag="AC16CX_18170"
     CDS             7113..7328
                     /locus_tag="AC16CX_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3014472.1"
                     /translation="MKTVVLTIEGMYCDGCAETIEGLLAVETGVKASTVSFKEGRARV
                     LYDPAVIDEKRLVAAIEKGGYKVASAA"
     gene            7325..7996
                     /locus_tag="AC16CX_18175"
     CDS             7325..7996
                     /locus_tag="AC16CX_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014473.1"
                     /translation="MTDPLIAAILLPIGLGLFGFIEPCSIGSTLVFIKVMEGKPAAIK
                     LWQVGMFTATRALFIGVLGAVAVLVGSAFIGFQKAMWTGLGVLYVALGVLFLAGRAGY
                     VMTSIGPRLARIGELRSSAALGLLFGLNIPACSAPLLFALLGTAAASGVSGATLANGF
                     LSLALFGFALSLPLAVAVLFPAAQRALDWLAGLSRRVPFWTGIVLVALGLWSVWFGLF
                     VTPRP"
     gene            8175..>8452
                     /locus_tag="AC16CX_18180"
     CDS             8175..>8452
                     /locus_tag="AC16CX_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3014474.1"
                     /translation="MITTSLSEPLLKLWTEEPAARPDRARIRARIGGLHCSLCTGTIE
                     KALGRLPGVDRVAVSLTHEQALVEFDPSVVQAEELLRTLRDIGYTVHDP"
BASE COUNT         1548 a         2719 c         2666 g         1519 t
ORIGIN      
        1 cgatgcgcgg caggatcggg tagccgaccg actggaacag ttgcgcgatc agcgtagagc
       61 aaatggcccg cgtcggctcg ccgctgccca gcgcgagcat gcgccgtcgc cagcgctgcg
      121 gaaccggggg tgtcgggatc aaatagcgcg ccaaatcgaa cacattgcgc aaatcgtagg
      181 tgtgccctat gcgggccacg gccgcttcga tgatccgctc gcaatccgtc tctgcgagcc
      241 ctacggggcg gcagatgcga gtgtgacatt gatcgtagtt gctgagcggc accgcccgaa
      301 cgcccccgac cagatcggcc tcgatcagaa ccggtggatc agggtcgtcc ttcgaatgtg
      361 caaggcgacg accgacgaag agcgcggcat gcgaccacgt ggactgcgtc aggtacttga
      421 tcgcgacgct gatgcgggta ttgccttcca ctagcagcac gtcacccgcg cgaagcgcct
      481 gccgcagctg cccggggggg ttggtggcca gcggctgata actcttgctc ggcctggtga
      541 gataggcagc gagcgcatgc cccagatacc ccggaaacgc ttgcatcggt gcaccctctg
      601 gccgtcagcg cctgtcacgc gcaggcgcca cgcgggggga gaagatctcc gactgcgggt
      661 tgaacgccag ccaggcgaat gcgaagtgca cgccgctgtc gaccggcttc aggcgctcgc
      721 ccttaagtgg tccggcgacc gccttgccga agatgtccca ctcggaaccg gtgtcgcggt
      781 cgaagacgcg ctcgcctcgc agttcgaaga ccagtgtacg atcacccagc ctgcggctgt
      841 aggccgccgc ggcgggaatt cggcgcgagt cccggatgag ctcggcgtcg agcgccgaca
      901 acatgccctc ggacgtgaag atcgcgacgg gctcccgtcc aacctcgtca ttgacgacgc
      961 gaatccccgc cagcgcggca aagggataca cgcgcacctt tccgctgatc gccacgccca
     1021 gcacgcgctc catggcgggc aggcgcggat ccaccggatc gtggaacagg aaggggatgt
     1081 cgccgacacg gtcgtagccg cgatagggat ttttcccgta tggacggctg aacccggtct
     1141 ggtgcgacag tacacgcccc tgcgggtacg cctttcgaaa gtccgagaac gggacgatgg
     1201 tcgacggcaa ttgctctagc gccgttcctg catgacggcc gacgatgcct ttgccggtga
     1261 actgctgcca ccagctctcg gtttggcggt catacatgac caggtcgctc ttgcgcaatt
     1321 tgccggtcgt gccgaaatcg agcaccacgc ccgctattcg cctgtcgaac acgagggacg
     1381 cgttgcacag cgggcagaac gtgacgacga ccgggatgct gcccacgatg tcgttgacga
     1441 tctcgtgaaa catcaggatc tgcaatggat acgcgcgcgc atcgccattg cgctcgaaca
     1501 cgatcacggg ctcacggcga tcaagccatt tgcccgcttc ttcgggcgtc acgaattgcg
     1561 gccggtcgat cgcgggaatg ccgtctttcg gcgggcctcc ggagtgaatt tcctcaaggg
     1621 aaacgatgcg cttgctgaaa tcggtgcgcg gccactcgct cgtcaatcgg gtgtcggccg
     1681 gcatggctac gctccaggtc aacagcgcgg caatggcaat cgaccaccgc gttccctcga
     1741 cggtgcactt cgcgtcgcgt gctcgcatgc cgattagttc ctcaggccga accagggcct
     1801 caatctcacg ccgatcgcat taccacccag cgcggcggcg atccacaccc aaccgtgtag
     1861 actggtggag gcgacgcccg agaaaaacgc gccgatattg cagccatagg cgatgcgggc
     1921 accgtagccc atcgccagcc ccccgatcac cgccgctgcg agcgacgcac cggataatcg
     1981 cagccggggc cggaatcgtc cggcagctcc cgccgccgcg ccgagcacca tgccgatgtc
     2041 catcaccgaa gttacatcct cgaaaaggct gctttcgagg gcccggtgct gaaaatcccc
     2101 gttccagaac gcgctctcat ccggctgcca gccgaatgaa agagcggttt tcgcgcccca
     2161 caacgcgaaa gcccacgtga tgctccaggg atgaccggcg acgagcagca cgaggaaggc
     2221 aagcacggcg agcaataccg cgccgagcag cagcgtgcgt tccctgacgg acgcgccgtc
     2281 caggcgcgat gagcgttccg cccggcccgg cgcaaccctt ccgagccaca cccagattac
     2341 ggcgagagcg cccagctgca gcgccagcgc ctccgtccag ccaagaaggt cgccgagcgc
     2401 ggcctcgccg cgcgacggca gccgttccca ccagctcatg tgcaggctcg cccagaacga
     2461 tccggcaacg aagaacgcaa gcacgatcag catcctcgcg cttcccccgc cggcggcgaa
     2521 cagcgcgccg gagccgcacc ctccggcaag ctgcatgccg attccgaaca tgaacgcgcc
     2581 tgcgacgacc tgcgcgccca ccggcgcgaa cgcgcctgaa accggttgac caaaagcgga
     2641 gcccgccgcc aatacgggga caaacagcac ggtggcgagc gccagcatca ggacctgcgc
     2701 ccgcacgcct gcggtatcgc ggtacagcag gaattcccgg taggccgagg taaagccaaa
     2761 cgacgtcaga agcagcacgg cgccgagcgc cattccgaga agaagcagcg cgggcatgcg
     2821 ccagccctgg cccaccagcg cgaaaagcag caggccggcc agtccaagcg tggcggcggt
     2881 ccacgcgtac tggatacgca gctgcactgc gccagcggcc tcaaccaccg gcggcgtcac
     2941 gcacgatatt ttcgaatatg acatcgagct ccttggccat ttcgtcgagc gctgaactcg
     3001 agtacggtgc aaacaccgtg ctcgggcggc ggtacgtcaa gctcgcgctg ccatcggcgt
     3061 tttccgtcac gtaaatgcgc aatggcgcct cgattcccgc cggtacgctt gccttgagca
     3121 tgcgcatcgc atagtcgttg cggaatacca tgagaaccgc gttgcccgga atcttcacgc
     3181 cgcgtgtcgc ggcaccgcgg cttgcactcg cttgagcaac caatcccatc ttgtgatccc
     3241 cgatggcttt ttcgatgcga gttagcatcg catcaaaccc gtggcgggtc ttgatggtca
     3301 ccgttcccgg cagtcctgtt tccgcaccct gcgacggtcc agcgccggcg agcgctagca
     3361 gacctgcgac cagcagtaga aatcgggccg atctcgaaac catgaatgcc cccagctgag
     3421 atgtaatacg cctgccggtt ccggctggat gctatcgcac gatcagtttg tagtcgggag
     3481 tggtattgag cgggcacgaa tagacatatt cgccgggctt gagctcgatt tcgtagtccc
     3541 gggttttccc tggcatgagg ccaccgccgg aaacgcttgg gagggtcaca cggcccgccg
     3601 cggatgcgcc gcgcaaccag aagcccagct cgtacggcac gttcttgttg gtgacgcgga
     3661 atacatattt cccaggtttg agctggagcg gcttggcttg cgccacgcgc tgctgggcgg
     3721 tcttggcgtt gatcgcctcg caatccttga tcgagcgtga cttgtagccg cgctcaacgc
     3781 cttcgctctc gaggaactgg cacggcagct gggtcagcac gatgatctgc gcagccgcag
     3841 cgggcgccgc gggcaggccc agcaccaatg cggagagccc taggcagcag aaagagccgc
     3901 ggcaactcgg gaatcgcttg accatgcgtc cggggctcct ttgacgggcg cggtgcgcca
     3961 attaaactgg attgcgaatg ccggccagtg cgcgaatttc acgcaccgtt tcttcgaccg
     4021 aaataggcag gaatcccgtc acctcgagtt cccgatcctc gaacgcgtct ctttcgggat
     4081 gggtcctcgt ccaggcttcg attaccgcgt cacggtggat cagcaaggcc tcgatgtacg
     4141 gccggaacag caccatcatc gccccgatcc agcggttgac cggccacgac ggataggcgt
     4201 gatctatgcg aaagcggggc aacagtccga ttgcctcctc cgccggcagc caagcgcccc
     4261 cgcaaaccca gtggttggtc gcaaacaggc cgaccggcca tccgtacgcg tccattgaga
     4321 tcgcgatgag atgcacaggc tcatcgctat cggggccgtt ccccgcaggc cagccgtcgc
     4381 ggttcccggt gctcacccgc aaaaacgtgt gaaagtgccc gtgttccccg ccacggtgtg
     4441 cgtggtagta atactgcgcg ccggattcgg agtcgtagac gtcactcctc gggtaatgct
     4501 cgtactcgac gaattctccc tggtcacgca agacctcgct taccaggttg atccctccat
     4561 gggacagcac acgctgacat tcgagcacct cggcgcccgc catgagcatg gcccttagtc
     4621 gacgcttcgg gagtgcgccg aaaacaggcg gcgacaattc ggcgccatct ggcggtaaag
     4681 tgggggcgag cggcgattcc acgccggttt cgcgtgtccc gcgtgcgtca ccgtgaacag
     4741 gggcggcgaa gttcatcttg ttcgggcgtg cgttgattgc cgtgaatgac gcggcggcat
     4801 gcccgcccaa tgcgtactgt caaccgtggc gcgttccacc tatcttttcg ccccgcaggg
     4861 attctttgct ccgcacggat ttttggctgc gcatggattt tttgctgcgc aggggttccg
     4921 cttggcagcg catgggtttg cccgggcgcc gcacgggctc ttggccgcgc agggattctt
     4981 cgccgcgcac ggattgggct tgaacttctt ctgcacgtca agcgtgtacg ccgtcaacgc
     5041 cgccagttcc ttcgactccc acttgagagg cttggccgcc atcggcacca ccatgcagaa
     5101 ctgcaccatt tcctcggcgg agacccgctt gaagcccccc ttctcctggg tcatggcgac
     5161 atggtgtgga tagggtttcg cgaacgtggc attgaacgcg ccgttgttga cgtgacaggt
     5221 gttgcacgcc atgccgttgg tgctgagctt ggtgtccttc cacagcacct cgccctcctt
     5281 gaccagcgtg gcgcggtcgg ctttatagac cttcgtgcct ttcggacgag tcaccagctt
     5341 ggggtcgacc ttgtcgcccg cgccgcaggg acttttcgcg ccgcacgggc ttttggctgc
     5401 gcacggattt ttcgccgcgc aggggttctt cgcggcgcaa gggtttttct tggctgcaca
     5461 tggattcttt gcggcacacg ggctggcacc cgagcgcgaa gcccgtggcc tggcgccgca
     5521 cgggctacag gggcttgccg caaagccggc aggcatgccg agagccgctg tcatcacgcc
     5581 tgcggaaacg agggaccgag tcatcggttt gagatcgaaa cgcatgtttg ttcctccttt
     5641 accgggttat ccgtttcagt tgctgattca tcacgatgta tcgcccagcc tgctgcgatg
     5701 tcgaggcaac acgataggtg tggagcagac gcaccccgcg cccccttgga atcagtagac
     5761 cggaataacg ggcaaaacct tacaaggctt ttcgcctgta tttcggccgg gctcggaaac
     5821 tcatctgccg cggtctggtc atcaacgtca tgaggtaaac gaggagacgc ggagacgggc
     5881 gaagcagttg tatagtttgc agggcgaacc ttgaccgcgc gaccctcggg aacatcgcag
     5941 cgaccatgct gcgccgcctc gaccgatgat ttcttgacag gagcatgata tggcccaatc
     6001 agtcaaaccc gatccgcggc tcgcgccgct gccggtggag gccacgccgg aattgaagaa
     6061 aacgtttcag acctatcaca agacgctcgg ctatgttccc aacagcgcgc tcatcatgca
     6121 gcgcaagccg aagctggtga aagcctttac ggcgctcacc gccgcggtgt gggacccgga
     6181 gagcgaggtg gaccgcggct tcaagcgctt gatcgcccat gtcgccagca ggacccacgg
     6241 ctgccattac tgaatggcgc acactgccgg agggtcgctc cggttcggca tcgaagagca
     6301 gaaacttgcc gcagtgtggg aataccgcgc gagtcccatg tatacggagg ccgagcgcgt
     6361 cgcgctcgat ttcaccatag cggcggcctc gcaacccaat gacgtgacgg atgagctgtt
     6421 tgcaaggctg aaacagcact ggaccgagaa ccagatcgtg gaaatcgtag cgaccatcgc
     6481 gctgttcggg tttctcaacc gctggaacga cacgatggcg acgccgctcg agagggagcc
     6541 gatcgaggtc gcggagaaat tccttgcgcc tcatggatgg aacatcggca agcatcaccg
     6601 ctgacgccgc gcgcggggtt gccgcccctt gaccctggac caaactccag ggtttaagct
     6661 attggtaact cgggccgtag cggaggggac atggacgcgc acagggcgga aatgaccatc
     6721 gggaaggtgg cgcgcaaagc cgggctggcg atcgataccg tgcgctatta cgagcgcgag
     6781 ggtctgatcg agaagccggc gcgcagcgca tcgggctacc ggaattaccg cccggacgtg
     6841 atcgcgcggt tgcgcttcat ccgccaggcc aagaacctcg gtttctccct gtccgaggtc
     6901 cgcgagctgc tcgccctgaa ggttgcgccg ggcaagtcct gcgcggatgt cagggcgcgt
     6961 acggagcgca agatcgccga cgtcgagcag cgcatcgcgc aactcgagcg gatgaagcgc
     7021 gcgcttgcga agctcgccgc cgcgtgctcc ggccgcggcc cgacgagcga atgcccgata
     7081 ctcgaggcca tggagacaca cgagatcagt caatgaagac ggtggtgctc acgattgaag
     7141 gcatgtactg cgacggctgc gccgaaacga tcgagggact gctcgcagtc gaaacggggg
     7201 tcaaggcgtc cactgtttcg ttcaaggagg gccgtgcgcg cgtgctgtac gaccccgcgg
     7261 tcattgacga gaaaagactc gtggccgcca tcgagaaagg cggctacaag gtcgcgtccg
     7321 ccgcatgacc gatccgctca tcgcggcgat cctgctgccg atcgggcttg gattgttcgg
     7381 tttcatcgag ccgtgctcca tcggctccac gctggtcttc atcaaggtga tggaaggaaa
     7441 gccggcagcc ataaagctct ggcaggtcgg catgttcact gccacgcgtg ccttgttcat
     7501 tggcgtactt ggtgcagtag cggtcctcgt aggctcggcc ttcatcggct ttcagaaggc
     7561 catgtggacc gggctcggtg tgctttacgt cgcgctggga gtgctttttc tcgccggtcg
     7621 cgcaggttac gtcatgactt cgatcggccc ccgcctcgcg cgcattggcg aactgcgcag
     7681 ttctgcggcg ctcggattgc tgttcgggct caacatcccc gcgtgctcgg caccgctctt
     7741 gttcgcgttg ctcggcacag ccgccgcgag tggcgtgtcg ggtgcgacgc tcgcgaacgg
     7801 cttcctgtcc ctggcgcttt tcggcttcgc gctgtcgctc ccactggcag tcgccgtgct
     7861 ttttcccgca gcgcagcggg cgctcgactg gctcgccggg ctgagtcggc gcgtaccatt
     7921 ctggaccggc atcgtcctgg tcgcgcttgg attatggtcg gtctggttcg ggctgttcgt
     7981 gaccccacgc ccctagcccg catgttgcat gactgccaag gttcgggctc atgcgatcaa
     8041 gccgcgcggg gagacgcctg cagcatgcgg gctgaccaaa ttccggattc tcaggcgttc
     8101 acttctggcg gcttgactct ggagtagcct ccagggtcta agctgaagtg cattgatatg
     8161 gcaggaggac gaccgtgatc accaccagtc tctccgaacc gctgctcaag ttgtggaccg
     8221 aggaaccggc agcccggccg gaccgcgccc gtatccgcgc gcgcatcggc ggactgcatt
     8281 gttcgttgtg caccggcacc atcgagaaag cgctcggcag actgcccggc gtggacaggg
     8341 tcgcggtgag cctgacccat gagcaggcgc tggtggaatt cgatccgagc gtggtgcagg
     8401 ccgaggagct gctgcggacg ctgcgcgaca tcggttacac ggtacacgac cc
//
LOCUS       DBNGWZ010000580         9833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1093162, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000580 DBNGWZ010000000
VERSION     DBNGWZ010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1093162"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="hpnC"
                     /locus_tag="AC16CX_18185"
     CDS             complement(<1..797)
                     /gene="hpnC"
                     /locus_tag="AC16CX_18185"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIR3014475.1"
                     /translation="MGVNHYENFPVASLLLPRELRFPIERIYRFAREADDFADEGSDA
                     PDVRLGKLAAYRSKLVMIGRGVTPDDPMFEDIARIVRQHDLPLQLFQDLLEAFSRDVV
                     KNRYADFAEVLDYCRRSANPIGRLLLHLFRRASEDNLGWSDSICSALQLVNFWQDVAI
                     DFAKNRIYIPQDEMKAYGVTERHIAEQRCDESWVALMNFQIGRARGMLRSGAPLGRAL
                     PGRLGLEIRATVQGGLRILEKLERVRCDMFRHRPALKWYDWPLILARA"
     gene            902..986
                     /locus_tag="AC16CX_18190"
     tRNA            902..986
                     /locus_tag="AC16CX_18190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:936..938,aa:Leu,seq:tag)
     gene            1104..2405
                     /gene="tig"
                     /locus_tag="AC16CX_18195"
     CDS             1104..2405
                     /gene="tig"
                     /locus_tag="AC16CX_18195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR3014476.1"
                     /translation="MQANLQTLSALERRLSVALPAAEIDSEVESRLKRLSRTVRMHGF
                     RPGKVPLKVVAQHYGPQVRQEVLGDAVQKSFGEAVRQQNLKIAGNPKFELEPLNEGAA
                     EFHYSATFEIYPEVVVGDISNAVIERPQLEVGEAEVDKTIEIMRKQRARFERVERAAQ
                     NGDRATLDFRGTLDGAEFQGSNDRDQQVVLGGGRLLPDFEANVVGMKAGESKAFDVRF
                     PDDYHGREVAGKTARFEVAVKQVAAPRLPEINAEFAKSLGVADGDLARMRAEVKANLE
                     REVKAKLKSRVRDQVIQVLLDATRIEAPKSLVQMETQRLQAGARHELAARGVQMTDHT
                     PLSPDLFEKQAQRRVSVGLILGELMRANNLYPRPEQVRTLVEEQAQSYERPEEVVKWF
                     YASPERLRDIESAAAENNVVAWALGVAKVADKPIGFDELMG"
     gene            2411..3034
                     /gene="clpP"
                     /locus_tag="AC16CX_18200"
     CDS             2411..3034
                     /gene="clpP"
                     /locus_tag="AC16CX_18200"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR3014477.1"
                     /translation="MTQTSEPRGLGLIPMVIEQSGRGERAYDIYSRLLKERVVFLVGP
                     VTEVTANLIVAQLLFLESDNPDKEISFYINSPGGSVSAGLAIYDTMQFVKPPVSTLCI
                     GGAASMGALLLAAGAKGKRYCLPNSRVMIHQPMGGFQGQASDVEIHAKEILYLRSRLN
                     DIMARHTGQDIKTIERDTDRDNFLSAEQALAYGIVDKVLVSRTESVK"
     gene            3077..4345
                     /gene="clpX"
                     /locus_tag="AC16CX_18205"
     CDS             3077..4345
                     /gene="clpX"
                     /locus_tag="AC16CX_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR3014478.1"
                     /translation="MSDKVGGEKLLYCSFCGKSQHEVRKLIAGPSVFICDECIELCND
                     IIREETQGEHGSKGAKSNLPVPKEIRAILDQYVIGQDLAKKILSVAVYNHYKRLKTLT
                     KNDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVMADATTLTEAGYVGEDVENI
                     IQKLLQKCDYDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLVEGTI
                     ASVPPQGGRKHPNQEFVQVDTTNILFVCGGAFDGLDKIIRARSEKSGIGFGAEVSSRD
                     NRRDITKVLREVQPEDLIKYGLIPEFVGRLPVVATLEELDEVALIQILTQPKNALLKQ
                     FQKMFGMEGVELEVREGALKSVARRALERKTGARGLRSILEHTLLNTMFDLPSLEGVV
                     KVVVDENTITSDAAPLLIYSDQPKVAGSAH"
     gene            4455..6854
                     /gene="lon"
                     /locus_tag="AC16CX_18210"
     CDS             4455..6854
                     /gene="lon"
                     /locus_tag="AC16CX_18210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3014479.1"
                     /translation="MPDETVPIQMPLLPLRDVVVFPHMVIPLFVGRPKSIKALETAME
                     AGKSIVLVAQKSAAKDDPSPDDLYRIGSIANILQMLKLPDGTVKVLVEGAQRARLHEI
                     IDLKTHYAVTVTPVAPDASGDHETEAMRRTMIQQFDQYVKLNKKIPPEILTSLAGIDE
                     AGRLGDTIAAHLPLKLEQKQEVLEMFGVKQRLEHLLKLVEGELEILQVEKRIRGRVKR
                     QMEKSQREYYLNEQVKAIQKELGEMDEGADIDEIEKRIKAAHMPKEARKKAESELKKL
                     KLMSPMSAEATVVRNYVDALVSLPWKKKSKISTDLVAAEKILNEDHHGLEKVKERIVE
                     YLAVQLRVDKLKAPILCLVGAPGVGKTSLGQSIARATNRKFTRMSLGGVRDEAEIRGH
                     RRTYIGSMPGKILQNMNRVGVRNPLFLLDEVDKMGMDFRGDPASALLEVLDPEQNHTF
                     VDHYIEVEYDLSDVMFVCTANTLNIPPALLDRMETIRLSGYTEDEKVNIALKHLLPKL
                     MKNHGLKPEELAISESAVRDIIRYYTREAGVRALERELSKICRKVVKTLVREGEERKV
                     SVSARNLDKYLSVRRYTYGMAEAKNQVGQVSGLAWTEVGGELLTIEAAIMPGKGKMTT
                     TGKLGEVMQESVQAALSVVRSRSRRLGIPSDFYQKNDIHIHLPEGATPKDGPSAGIGI
                     CTAMVSALTGIPVRADVAMTGEITLRGEVLQIGGLKEKLLAAHRGGIKTVLIPEENVK
                     DLAEIPENIKNRLDIHPVKWIDSVFEMSLERKPEPLAEDEVAPVLPPVTEAPAPAAVK
                     H"
     gene            complement(7020..7235)
                     /locus_tag="AC16CX_18215"
     CDS             complement(7020..7235)
                     /locus_tag="AC16CX_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014480.1"
                     /translation="MKKAKRKIFSGWIAGERRYEIRWLLYSKREKWLSSKCKKFSAKM
                     LRDVAWNDTENGAGARANSRSQAKSRN"
     gene            7303..7575
                     /locus_tag="AC16CX_18220"
     CDS             7303..7575
                     /locus_tag="AC16CX_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR3014481.1"
                     /translation="MNKADLVEVVARSAGISKSASEKALDGAIDAIKGALRRGQSVTL
                     VGFGTFRVGRRQARLGRNPRTGAEIRIQAAKVPKFSAGKALKDALN"
     gene            7851..7926
                     /locus_tag="AC16CX_18225"
     tRNA            7851..7926
                     /locus_tag="AC16CX_18225"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7884..7886,aa:Val,seq:tac)
     gene            8018..8094
                     /locus_tag="AC16CX_18230"
     tRNA            8018..8094
                     /locus_tag="AC16CX_18230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8052..8054,aa:Asp,seq:gtc)
     gene            8233..>9833
                     /locus_tag="AC16CX_18235"
     CDS             8233..>9833
                     /locus_tag="AC16CX_18235"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR3014482.1"
                     /translation="MFDFVTKNKRLIQIVLAIIFLPFAFFGVDIYFRDMGSAQMVARV
                     GSYAISQDEFSRALRERQQALQRALEGRIDPAVLDNPELRFATLETLIQRRLLLDRAQ
                     SAGITVPDHYLKNAIGEQSLFRDETGKFSYPRYEQFLRSEGMTPVMFEARLRQDLILG
                     QFSDGYADSGFVPRTVTERLVRLSEQRREISHATIAPDRFLAQVKLAAGAAKQYYDAN
                     AGEFRVPEQVRVEYVTLSIESLMEGIRVDPGEVKKYYDTNRKQFAVEESRQAGHILVS
                     VDAGGGAEAKQKARARAEEIYNQVQKNPAGFAEAAKKYSQDPGSAARGGDLGTISRGS
                     MKDAPEFESALYKLKPGEISPPVETRHGFHIIHLVAVRPAQVKEFDEVRGQIEQELRK
                     QLAGRRFAEVADHFNNIVYEQSESLKPAADLIKAAPRQSGWITRASAEAPLNNARLLA
                     AIFSEETLKNKRNTEGIEVAPGTLIAARVIEYKPESVQPFEELRAALEKRLALREAAR
                     LAAEEGQRLLAELRQGKSAGIAWSAP"
BASE COUNT         2048 a         2948 c         3206 g         1631 t
ORIGIN      
        1 gcgcgcgcca gaatcaacgg ccagtcgtac cacttcaagg ctggccggtg gcgaaacatg
       61 tcgcagcgca cgcgctcgag tttttccagt atgcgcagcc cgccctgcac ggtggcgcga
      121 atctcgagac cgaggcgccc aggcagcgcc cgcccgagag gggcgcccga ccgcagcatt
      181 ccgcgcgcgc gcccgatctg aaagttcatg agcgcgaccc agctctcatc acagcgttgc
      241 tcggcgatgt gccgctccgt aacgccatag gccttcatct cgtcctgcgg aatatagatg
      301 cggttcttgg cgaagtcgat cgccacatcc tgccagaagt tgacgagctg cagcgccgaa
      361 cagatcgaat cggaccatcc gagattatcc tcgctcgcgc gccgaaagag atgcagcaac
      421 agccgcccga tcggattcgc cgagcggcgg cagtaatcga gcacttccgc gaaatcggcg
      481 taacggtttt ttacgacatc ccgcgaaaag gcttccagca gatcctgaaa aagctgcaaa
      541 ggcagatcgt gctggcgcac gatacgggcg atatcctcga acatcggatc atcgggcgtt
      601 acgccgcgcc cgatcatgac cagcttcgac cgataggcgg caagctttcc caaacgcacg
      661 tccggcgcat ccgacccctc gtcggcgaag tcgtcggctt cccgcgcgaa acggtaaatg
      721 cgctcgatcg gaaagcgtaa ctcgcggggt aaaaggagcg aggcgaccgg gaaattttcg
      781 tagtggttga cgcccatttg ctgctacgca atatcccatt attatcagag ggctgcgttg
      841 caaccttaag acaacttgca agctttcggt gcgcacctga ttatattaca atcagaaagt
      901 cgcgaaagtg gcggaattgg tagacgcacc agatttaggt tctggcgccg caaggcgtgg
      961 gggttcgagt ccctcctttc gcaccacggg cggaccgcgc gtggtgcgag tcggtgcgtc
     1021 gatccgggtt tcgcccttcc cgagagcacc gtttgcgggg cagccccgga gcaggcgttg
     1081 ccgggattaa tcaagaacga gagatgcaag ccaatttgca aacactgagc gcactcgaac
     1141 ggagattgag cgtcgccctg cccgccgccg aaatcgacag cgaggtggag agccgcttga
     1201 agcgcctgtc gcgcacggtg aggatgcacg gctttcgccc cggcaaggtg ccgctcaagg
     1261 tggtcgcgca gcactacggc ccgcaggtaa ggcaggaagt cctgggcgac gcggtgcaga
     1321 aaagctttgg cgaggcggtg cggcagcaga acctgaagat cgcgggtaat ccgaagttcg
     1381 aactggagcc gctcaacgag ggcgccgcgg aattccacta cagcgccacc ttcgagatct
     1441 accccgaggt ggtggtggga gacatctcga acgccgtaat cgagcgcccg cagctggagg
     1501 tgggcgaggc ggaagtggac aagaccatcg agatcatgcg caagcagcgc gcccgcttcg
     1561 agcgggtgga acgcgccgcg cagaacggcg atcgcgcaac gctcgatttc cggggcaccc
     1621 tggacggcgc ggaattccag ggctcgaatg acagggacca gcaggtggtg ctgggcgggg
     1681 ggcggttgct gccggatttc gaagccaacg tggtcgggat gaaagcgggc gagtcgaagg
     1741 cgttcgatgt gcgatttccc gacgattatc acggccggga agtggcgggg aaaaccgccc
     1801 gattcgaagt cgcagtcaaa caggtcgccg cgccgcgcct gcccgagatc aacgccgagt
     1861 ttgcgaaaag cctgggcgtt gcggatggcg atctcgcccg gatgcgcgcc gaggtcaagg
     1921 cgaatctcga gcgcgaagtc aaggccaagc tcaagagccg ggtgagggac caggtgatcc
     1981 aggtgctgct cgacgccacc cgtatcgagg ccccgaaatc gctggttcag atggagaccc
     2041 agcggttgca ggctggcgcg cggcacgaac tggcggcgcg cggcgtgcag atgacggacc
     2101 atacgccgct gtcccccgac ctgtttgaaa agcaggcgca gcgccgggtc agtgtcggtc
     2161 tgatccttgg cgagctgatg cgcgcgaaca acctttatcc caggcccgaa caggtgcgga
     2221 cgctggtgga ggaacaggcc cagagctacg agcggcccga ggaagtcgtg aagtggtttt
     2281 acgcgtcgcc ggaacggctg cgcgacattg aatccgcggc ggcggaaaac aacgtggtgg
     2341 cgtgggcgct tggcgtcgcg aaagtggcgg acaagccaat cggtttcgac gaattgatgg
     2401 ggtagcggtg atgacacaaa ccagcgaacc aaggggtctc gggctgatcc cgatggtcat
     2461 cgagcagagc ggacggggcg aacgcgccta tgacatttat tcgcgcctgc tcaaggagcg
     2521 cgtggtgttc ctggttggcc cggtgaccga ggtgaccgcc aacctgatcg tcgcacagct
     2581 gctgttcctc gagtcggata atcccgacaa ggagatctcg ttctacatca attctccggg
     2641 agggtcggtt tcggcggggc tggcaatcta cgacaccatg cagttcgtga agccccccgt
     2701 gtccacgctg tgcatcgggg gtgcggcgag catgggtgcg ctgctgctgg cggcgggcgc
     2761 caagggcaag cgctactgcc tgcccaactc gcgggtgatg atccaccagc cgatgggcgg
     2821 cttccagggg caggcctcgg acgtggaaat tcacgccaag gaaatcctct acctcaggtc
     2881 gcgactcaac gatatcatgg ccaggcacac cggccaggat atcaagacca tcgagcggga
     2941 taccgaccgt gacaatttcc tcagtgccga gcaggcgctg gcatacggga tcgttgacaa
     3001 ggttctggtg agcagaaccg aaagcgtaaa atgatgtcgt atttaaacag gaatgtcgcg
     3061 ctcttgggga cggcgtatgt ctgacaaagt cggcggggaa aaactgctct attgctcgtt
     3121 ctgcggcaag agccagcacg aggtgcgcaa gctcattgcg ggaccgtcgg tgttcatttg
     3181 cgacgaatgc atcgagctgt gcaacgacat catccgcgag gagacccagg gtgagcacgg
     3241 gtcgaagggc gcgaaatcca acctgccggt accgaaggag attcgcgcga tcctcgacca
     3301 gtacgtgatc ggccaggacc tcgccaagaa gatcctgtcg gtggcggtct acaaccacta
     3361 caagcggctg aagacgctca ccaagaacga tgacgtcgag ctcgccaagt cgaacatcct
     3421 gctgatcggc ccgaccggtt cgggcaagac actgcttgcc cagacgctcg cgcggctgct
     3481 caacgtcccg ttcgtgatgg cggacgcgac cacgctgacc gaggcgggtt atgtcggcga
     3541 ggacgtcgag aacatcatcc agaagctgct gcagaagtgc gactacgacg tcgagaaggc
     3601 gcagcgcggc atcgtctaca tcgacgagat cgacaagatc tcgaggaaat ccgacaatcc
     3661 ctcgatcacg cgggacgtct ccggcgaggg cgtgcagcaa gcgcttctga agctcgtcga
     3721 aggcaccatc gcctcggtgc cgccgcaggg cgggcgcaag caccccaacc aggaattcgt
     3781 gcaggtggac accaccaata tcctgttcgt ctgcggcggc gcgttcgacg ggctggacaa
     3841 gatcatccgc gcgcgctccg agaagagcgg catcggcttc ggcgcggagg tgagctcccg
     3901 cgacaaccgc agggacatca ccaaggtgtt gcgcgaggtg cagcccgagg acctcatcaa
     3961 atatgggctc atccccgagt tcgtgggacg gctgccggtg gtggcgactc tcgaggagct
     4021 ggacgaggtt gcgctgatcc agatcctgac gcagccgaag aacgcgctcc tcaagcagtt
     4081 ccagaagatg ttcggcatgg agggcgtcga gttggaggtg cgggaaggag cgctcaagtc
     4141 cgtcgcgcgg cgcgcgctcg aacgcaagac cggggcgcgg gggctgcgct ccatcctcga
     4201 gcacacgctg ctcaacacca tgttcgacct gccttcgctc gagggggtgg tcaaggtggt
     4261 ggtggacgag aacaccatca cctccgatgc cgccccgctg ctgatttatt ctgaccaacc
     4321 caaggtcgcg ggttcggccc actgagtgcg caccgcccgc gaaatcggtt tggaatcagg
     4381 cgcgtgcgtt ttgcgcttga attttctggg gtttccccaa tctagagctt attcaaactg
     4441 cccggtgaga cacgatgcct gacgagaccg tcccgatcca aatgccgttg ctgccgctgc
     4501 gcgacgtcgt ggtgttcccg cacatggtga ttccgctgtt cgtggggcgc ccgaagtcga
     4561 tcaaggcgct cgaaaccgcc atggaggcgg gcaaaagcat cgtgctggtc gcccagaagt
     4621 ccgcggccaa ggacgatccg agcccggacg acctctaccg gatcggcagc atcgccaaca
     4681 tcctgcagat gctgaagctt cccgacggca ccgtgaaagt gctggtcgag ggggcccagc
     4741 gcgcgaggct gcacgagatc atcgatctca agacgcacta cgccgtcacg gtcacgccgg
     4801 tcgcccccga cgccagcggc gatcacgaga ccgaggcgat gcggcgcacg atgatccagc
     4861 agttcgacca gtacgtgaag ctcaacaaga agatcccgcc cgagatcctc acctcgctcg
     4921 cgggaatcga cgaggccggc cggctcggcg acaccatcgc cgcgcacctg ccgttgaagc
     4981 tcgagcagaa acaggaggtg ctcgagatgt tcggcgtcaa acagcggctc gagcacctgt
     5041 tgaagctcgt cgagggcgag ctcgagatcc tgcaggtcga gaagcgcatc cgcgggcgcg
     5101 tcaagcgcca gatggagaag agccagcgcg agtactacct caacgagcag gtcaaggcga
     5161 tccagaaaga actcggcgag atggacgaag gcgcggacat cgacgagatc gagaagcgca
     5221 tcaaggccgc gcatatgccc aaggaggccc gcaagaaggc cgaatcggag ctgaagaagc
     5281 tgaagctcat gtcgccgatg tcggcggagg cgaccgtcgt gcgcaactac gtcgacgcgc
     5341 tggtgtcgct gccgtggaaa aagaagagca agatcagcac tgatctcgtg gcggcggaga
     5401 agatcctgaa cgaggaccac cacggtctgg aaaaggtcaa ggagcgcatc gtcgagtacc
     5461 tcgcggtgca actgcgcgtg gacaaactga aggcgcccat cctgtgcttg gtcggcgccc
     5521 ccggcgtggg caagacctcg ctcggccagt cgatcgcgcg cgcgaccaac cgcaagttca
     5581 cacgcatgtc gctgggcggc gtgcgtgatg aagcggagat ccgcgggcac cgccgcacct
     5641 atatcggctc catgccgggc aaaatcctgc agaacatgaa ccgcgtgggc gtgcgcaacc
     5701 ccttgttcct gctggatgaa gtggacaaaa tggggatgga cttccggggc gacccggcga
     5761 gcgcgctgct cgaggtgctc gatcccgagc agaaccacac cttcgtggac cactacatcg
     5821 aggtcgagta cgacctctcc gacgtgatgt tcgtgtgcac cgccaatacg ctcaacatcc
     5881 cgccggcgct gcttgaccgc atggaaacca tccggctctc cggctacacc gaggacgaga
     5941 aggtcaacat cgcgctgaag cacctgctgc ccaagctcat gaagaaccat ggcttaaagc
     6001 ccgaggagct cgctatctcg gagagcgcgg tgcgcgacat catccgctac tacacgcggg
     6061 aggccggcgt gcgggcgctc gagcgggagc tctccaagat ctgccggaag gtggtcaaga
     6121 ccctggtgcg cgagggcgag gagcgcaagg tgtccgtgag tgcccgcaac ctcgacaagt
     6181 acctgagcgt gcggcgctat acctacggca tggccgaggc gaagaaccag gtcgggcagg
     6241 tatcggggct cgcctggacc gaggtcgggg gagaactcct gacgatcgaa gcggcgatca
     6301 tgcccggcaa gggcaagatg accaccaccg ggaagctcgg cgaggtgatg caggagtcgg
     6361 tgcaggcggc gctgtcggtc gtgcgcagcc gctcgcggcg ccttggcatc ccgtcggact
     6421 tctaccagaa gaacgacatc cacatccacc tgcccgaggg cgcgacgccc aaggacggcc
     6481 cgagcgccgg cattggcatc tgcaccgcga tggtgtcggc gctgaccggc atcccggtgc
     6541 gcgcggacgt ggcgatgacc ggcgagatca ccctgcgcgg tgaggtgctg cagatcgggg
     6601 ggctcaagga gaagctgctt gccgcgcacc gcggcggcat caagaccgtc ctgattcccg
     6661 aggagaacgt gaaggacctc gcggaaatcc cggaaaacat caagaaccgg ctcgacatcc
     6721 acccggtcaa gtggatcgac agcgtgttcg agatgtcgct cgagcgcaag cccgagccgc
     6781 tcgcggaaga tgaagtcgcg ccggtcctgc cccccgtcac cgaggcgccc gcgcccgccg
     6841 ccgtcaagca ctgaacgccg tgtgaccggt gactggtgac cagtgaccgg tgaagcgtaa
     6901 gcaaaagggc ggctctgccg ccctttttgt tttcgccgta ccgcttcaag cgcaagcacg
     6961 acgcgtgccg agtcgcctct aacgccgctc gcaactcatt cattcaatcg ctcaatcact
     7021 taattccggc tcttcgcttg acttcttgag ttagcgcgtg ctcccgcgcc attttctgtg
     7081 tcgttccacg caacgtcacg taacattttt gcggaaaatt ttttgcattt acttgacagc
     7141 cacttttcgc gcttgctata aagtagccaa cgtatttcat aacggcgctc gccggcaatc
     7201 caaccagaaa atattttccg cttggccttc ttcattcgtc gcgtgaaggt tcagatagcg
     7261 ggggagactc atttccgtca ctgtttgcga aaggggacaa taatgaacaa agccgatctg
     7321 gtcgaagtcg ttgcaaggtc cgcaggcatt tccaagtccg cctcggagaa ggcgctcgat
     7381 ggggcgatcg atgctatcaa gggcgcattg aggcgtggcc agtcggtcac gctggtcggt
     7441 ttcggcacgt tccgagtggg caggcgccag gcgcgcttgg gacgcaaccc gcggaccggc
     7501 gcggaaatcc gtatccaggc ggcgaaagta cccaagttca gcgctggtaa ggcattgaaa
     7561 gatgcgctaa actagccacc aggctgcaat ccggcggtat ccaattgggg tgggcgcggg
     7621 gatcctcgcg gcctgcccca aaatgtttcg ggggcgctgg ggccggcggt aacaaacaga
     7681 ttgtcggcgc gcacgttgag cggcagtgcg ctgtccgacc ggagacgctg gcttgagaca
     7741 gcacaacagc gatgcgtggg tgcgtgccgg gtcagtagtg tgcgttgagt ggcagtattc
     7801 tgtccgctcg aacgcgcaca cttaaaacgg cgcacgagcg tccgttttaa gggtgcttag
     7861 ctcagctggt agagcgtcgc ccttacaagg cgaaggtcgg gagttcgagc ctctcagcac
     7921 ccaccagact tcagtgatga gtgcagagtg cagaatgaag ggcggatcgg cggggttcca
     7981 ttcatcactc atcactctgc attccgcact gtattacgga gcggtagttc agttggttag
     8041 aatgccggcc tgtcacgccg gaggtcgcgg gttcgagtcc cgtccgctcc gccagattgc
     8101 gaaaagggcg aacgacgttc gcccttttcc ttagtgagta gtgattggtg agtcgagagt
     8161 cgagaaacag cctaaagcgc gagacaatag tccactcacc actcaccatt caccactcac
     8221 ccttgttgaa agatgtttga cttcgtcacc aagaacaaac gcctgatcca gatcgtcctg
     8281 gcgatcatct tcctgccgtt cgcgttcttc ggcgtggata tctattttcg cgatatgggg
     8341 tcggcgcaga tggtggcgcg ggtcggcagc tacgccatct cgcaggacga attcagccgg
     8401 gcactgcgcg agcggcagca ggcgctgcag cgcgcgctgg aggggcgcat cgatccggcc
     8461 gtgctggaca acccggaatt gcgctttgcc accctcgaga ccctgatcca gaggcgcctg
     8521 ctgctcgacc gggcgcagag cgcgggaatc acggtgcccg atcattacct gaaaaacgcc
     8581 atcggcgagc agtcgctgtt ccgcgacgag accggaaagt tttcctatcc gcgctacgag
     8641 caattcctga ggtccgaggg catgacgccg gtgatgttcg aggcacgcct gcggcaggac
     8701 ctgatcctcg ggcagttctc cgacggttat gccgacagcg gattcgtgcc gcgaaccgtc
     8761 accgagcgcc tcgtgcgcct gtccgagcag cggcgcgaga tcagccacgc cacgatcgcg
     8821 cccgacaggt tcctcgccca ggtcaagctt gctgcggggg ccgcgaagca atactacgac
     8881 gccaacgccg gcgagttccg ggtgccggag caggtacgcg tggagtacgt gacactttcg
     8941 atcgagtcgc tgatggaggg aatccgggtt gacccgggtg aagtgaaaaa gtactacgat
     9001 accaaccgca aacagttcgc ggtcgaggaa tcgcgccagg cgggccatat cctggtgagt
     9061 gtcgatgcgg gggggggcgc ggaagcgaaa cagaaggcac gggcgagagc ggaggagatc
     9121 tacaaccagg tccagaagaa tccggccgga ttcgccgagg ccgcgaaaaa gtactcgcag
     9181 gatccgggtt cggcggcgag gggcggcgat ctgggcacca tcagccgggg ctcgatgaag
     9241 gatgcgcccg agttcgagag cgcgctctac aagctcaagc cgggtgaaat ctcgcccccc
     9301 gtggaaacac gccacggctt ccacatcata catttggtcg ccgtccgtcc cgcccaggtg
     9361 aaggaattcg acgaggtgcg cggccagatc gagcaggagc tcagaaaaca gctggcggga
     9421 cggcgcttcg ccgaggtggc ggaccacttc aacaacatcg tctacgagca gtcggaaagc
     9481 ctcaagccag ccgcggacct gatcaaggcg gcgccgcgcc aaagcggctg gattacccgc
     9541 gcaagcgccg aggccccgct caacaacgcg cggctcctcg ccgcgatctt ttccgaagag
     9601 acgctcaaga acaagcgcaa caccgagggg atcgaggtcg cgcccggcac cctgatcgcg
     9661 gcgcgagtca tcgaatacaa gccggagagc gttcagccgt tcgaagagct gcgcgccgcc
     9721 ctcgagaaaa ggctggcgtt gcgcgaggcg gcgcgcctcg cggcggaaga ggggcagcgc
     9781 ctgctcgccg agctcaggca gggcaagagc gccgggatcg cgtggagcgc gcc
//
LOCUS       DBNGWZ010000581         2619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1597299, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000581 DBNGWZ010000000
VERSION     DBNGWZ010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1597299"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16CX_18240"
     CDS             complement(<1..404)
                     /locus_tag="AC16CX_18240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3014483.1"
                     /translation="MVRQTPAVLAFAVAVALFLAGGASAATPGALPDIPTPYLPSTSV
                     AVDEVLRLAGVGPDDLVVDLGSGDGRVVSAAARDYGARGLGIEIDPKLVAESQAKARQ
                     AGVAGRVAFRHGDVLVADYRDATVVTLYLLPSL"
     gene            complement(651..1583)
                     /locus_tag="AC16CX_18245"
     CDS             complement(651..1583)
                     /locus_tag="AC16CX_18245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014484.1"
                     /translation="MKHPIPVSARTARLGCAFLVTCFAATPALAQDWGDTPYVQTPQN
                     VVDRMLEIAKVGPGDYVIDLGSGDGRMVITAAKKHGARGFGVDLDRKLVALANRNARK
                     AGVAERAVFYQRDLHETDVSPASVLTIYLLPEVNLMIRHRLLSTLKPGTRIVSHDYDM
                     GEWPPDFQTELAAPGKTVGIGQRSKIFYWMVPGRAAGRWLWRLAPEGRPIEFALSLQQ
                     NFQVIEGTLDAGGRTIRMENAKLAGEAISFSATLDGSRHDYSGRIVNHAIEGKARLTR
                     GGETRELAWSAARVEIWEPRHAALTREQALKEIH"
     gene            complement(1583..2539)
                     /locus_tag="AC16CX_18250"
     CDS             complement(1583..2539)
                     /locus_tag="AC16CX_18250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR3014485.1"
                     /translation="MPTLSWQHIPRLPLKIAAAACALPLAFVSLAWAGEARVPYVPTP
                     QEVVERMLETAKVGPGDYLIDLGSGDGRIVITAARKHGARGFGVDINPERIRESNDNA
                     RNADVTDRVAFYQQDLFATDLSQATVITMYLLPQVNIELRPKLLELAPGTRVVSHDFD
                     MGDWKPDVHLTVDAKDKYGGAGGTSDIYFWVVPARVAGVWRWQAPVAGKPQSYEVRLE
                     QKYQAITGSARVGGREVRLQGARLRGADIRFSFTADVNGAPVKHQFTGKVEGDVINGT
                     ASLSGTRIQGQFEWNARRTTPSSTGQRELRLWFSQSALSTFH"
BASE COUNT          436 a          886 c          837 g          460 t
ORIGIN      
        1 agcgaaggca ggaggtacag cgtgacgacc gtggcgtcgc ggtagtccgc tacgagcacg
       61 tcgccgtgac ggaacgcgac gcgcccggcc acgcccgcct gccgcgcctt ggcctggctt
      121 tccgcgacga gcttggggtc gatctcgatg ccgagcccgc gcgcgccgta gtcgcgcgcc
      181 gcggcgctca ccaccctgcc gtcgcccgag ccgagatcca ccaccaggtc atcggggccg
      241 acgccggcaa gccgcagtac ctcgtccacc gcaaccgatg tggaaggcag atacggcgtt
      301 ggaatgtccg gaagcgcgcc cggcgtggcc gcgctcgcgc cgccggcaag aaacaacgcc
      361 accgccaccg caaacgccag gaccgcggga gtttgccgga ccaaattccg gcaaactcct
      421 gatgcaaaac cgcccaagga aaaatcaaaa acaaggaact gcggagatac acgcttgatc
      481 ccgttcgcac ttcctaggtg gatcaacagc gtcattggaa ttgacttcat tcaagattct
      541 cgggcggttt tgctttcagc agcctgtccc cgatgcgtcg gtccgccgcg atacgttgac
      601 gccgcgcgct tcggcaaata gggccaagtc cgacaggctg cttgtgcgcg tcaatgaatt
      661 tccttcagag cctgctcccg ggtgagggca gcgtgccggg gctcccagat ttccacgcgc
      721 gccgcggacc acgcgagctc ccgcgtctcg ccgccgcgag tgagccgcgc cttgccctcg
      781 atcgcgtggt tgacgattct tcccgaataa tcgtggcgag atccatccag ggtcgcagaa
      841 aagctgatgg cttcgcccgc gagtttggca ttttccatgc ggatggtcct tccacccgca
      901 tcaagcgtgc cctcgatcac ctggaaattc tgctggagcg agagcgcgaa ctcgatcggc
      961 ctgccctccg gtgcaagccg ccacagccag cgtccggcgg cgcgccccgg caccatccag
     1021 tagaagatct tgctgcgctg gccgatgccg acggttttgc cgggcgccgc cagctcggtc
     1081 tggaagtccg gcggccactc gcccatgtcg taatcgtggg acacgatgcg cgttcccggc
     1141 ttgagcgtgg acagcagcct gtgccggatc atgaggttca cttccggcag caggtagatc
     1201 gtgaggacgg aggcggggct cacgtccgtc tcgtgcaggt cgcgctggta gaacaccgcg
     1261 cgctccgcca cgccggcctt gcgcgcgttg cggttggcga gcgccaccag cttgcggtcg
     1321 aggtccacgc cgaagccgcg cgcgccgtgc ttctttgccg cggtgatcac catgcgtccg
     1381 tcgccggagc cgagatcgat cacgtagtcg ccggggccga ccttcgcgat ctcgagcatc
     1441 cggtccacca cgttctgcgg cgtctggacg tacggcgtgt cgccccaatc ctgcgcaagc
     1501 gcgggcgtcg ctgcgaagca ggtgactaga aacgcgcagc cgagccgcgc cgtcctcgcg
     1561 cttaccggta tcgggtgttt cattaatgga atgtgctcag tgcggattgc gagaaccaga
     1621 gccgcaactc acgctggcct gtcgaagaag gggtagtgcg ccgcgcattc cattcgaact
     1681 gtccctggat gcgcgtgccg gaaagcgagg cggtgccgtt gatcacgtcg ccctcgacct
     1741 tgccggtgaa ctggtgcttc acgggcgcgc cgttcacatc ggcggtgaag ctgaaccgga
     1801 tgtccgctcc ccgcaagcgc gcgccctgca gcctgacctc gcggccgccg acccgggcgg
     1861 agccggtaat cgcctggtat ttctgctcga gcctgacctc gtaggactgc ggcttgcccg
     1921 ccaccggcgc ctgccatcgc cagacgcccg cgacccgcgc cggtacgacc cagaaataga
     1981 tgtcgctcgt gccgcccgct ccgccgtact tgtccttggc gtccaccgtg aggtgaacgt
     2041 ccggtttcca gtcgcccatg tcgaagtcgt gcgacaccac gcgcgtgccg ggagcgagtt
     2101 cgagcagctt cggccgcaac tcgatattga cctgcggcag cagatacatc gtgatcaccg
     2161 tggcctggct caaatcggtg gcgaacaggt cctgctggta gaacgcaacg cggtcggtca
     2221 catccgcgtt gcgcgcgttg tcgttgcttt cccggatgcg ctcgggattg atatccactc
     2281 cgaaaccgcg cgcgccgtgc ttcctggcgg cggtgatgac gatgcggccg tcacccgagc
     2341 cgaggtcgat taggtaatcg ccgggcccga ccttggcggt ttcgagcatg cgctccacga
     2401 cttcctgcgg cgtcggcacg tacggcaccc tggcctcgcc cgcccatgcc agcgatacga
     2461 aagccagtgg caacgcgcac gccgcggccg cgatcttcag ggggagccgg gggatgtgct
     2521 gccaggatag cgtcggcacg aacgcgctca atttgtgtgc gtctgtggtt aaattcgggt
     2581 tttgcgccgg gcaaaacccg aaagtcgcgg attataccc
//
LOCUS       DBNGWZ010000582         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2102044, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000582 DBNGWZ010000000
VERSION     DBNGWZ010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2102044"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="AC16CX_18255"
     CDS             <1..1403
                     /locus_tag="AC16CX_18255"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3014486.1"
                     /translation="RMGYVCNPSLHQNYTVAEVVQLLERIQSAALFVHPGYGADADRN
                     DIFTATRFVAALKRVYRLDPSGAHAAALDGLAIFPALGVEASPAPLPEPDLNPDKIMY
                     LAFTSGTTGQPKGVLHSDNTLLANGRAMVKDWGHDERTVLYSHSPLSHHIATVAVAQS
                     LVAGCELVVNDPPPGKRPLDWIVETGSTYVMGVPTHAIDILSEMKRRGLKQLGRVNIF
                     YMAGSVIPPETARAFLDLGIKPQNIYGMTENGSHQYTMPDDDVEVITTTCGKACHGYE
                     TRLWDPENPDREAASGAIGEIGTRGALLMLGYFDNQSATEGSFNASGWFMSGDLGRFD
                     AGGNLQIVGRKKDLIIRGGYNIHPARIENLALKHPAVLKAAAFPVPDERLGERACLAI
                     IARDGTRVDGDELLRHLDTAGLSKYDMPEYFIALDAFPLTASGKILKRELVEWAKSGR
                     IRPAPVRWTTSERKKA"
     gene            1501..>1913
                     /locus_tag="AC16CX_18260"
     CDS             1501..>1913
                     /locus_tag="AC16CX_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3014487.1"
                     /translation="MAIELTRVEEFALITLNRPEALNALSFALIAELARAFDEVAASN
                     ARALLITGAGAKAFCAGADIKELTGRPLADQKRGAELGQATFAKLDRLPVPSVAIVNG
                     YAFGGGLELALACTFRLATRNAKMGLPEIKLGLIPG"
BASE COUNT          352 a          644 c          604 g          313 t
ORIGIN      
        1 cgcgcatggg ctatgtgtgc aacccctcgc ttcaccagaa ctacacggtg gccgaggtgg
       61 tgcagctgct cgagcgcatc cagagcgccg cgctgttcgt gcaccccgga tacggcgcgg
      121 acgccgatcg gaacgacata ttcacggcaa ctcggttcgt cgctgcgctg aagcgcgtct
      181 atcgcctcga cccgtcgggc gcgcatgcgg cggcgttaga cggcctcgcg atttttcccg
      241 cacttggagt cgaagccagc ccagcgccat tgccggagcc ggatttgaat cccgacaaga
      301 tcatgtacct cgcattcacg tcggggacga ccggacagcc gaagggtgtg ctgcattccg
      361 acaacacctt gctcgccaac ggccgcgcca tggtgaagga ctggggccat gacgagcgca
      421 ccgtgctcta cagccacagc ccgctctcgc atcacatcgc cacggtggcg gtcgcgcaaa
      481 gcctcgtcgc cggttgcgag ctggtggtca acgatccgcc gccgggaaag aggccgcttg
      541 actggatcgt cgagaccggc tcgacctacg tgatgggcgt gccgacgcac gcgatcgaca
      601 tcctctcaga aatgaagcgc cgcgggttga agcagctcgg ccgggtgaac attttctaca
      661 tggccggttc ggtcattcca cccgagacgg cacgtgcgtt tctcgatctc ggcatcaagc
      721 cgcagaacat ctacggcatg accgagaacg gctcgcacca gtacacgatg ccggacgacg
      781 acgtcgaggt catcaccacg acttgcggga aggcgtgcca cggctacgaa acccggctgt
      841 gggacccgga aaaccccgac cgcgaggccg cgtccggcgc gatcggcgag atcggaacgc
      901 gcggcgcgtt gctgatgctc ggctatttcg acaaccagtc ggcgacggag ggctcgttca
      961 acgcctcggg ctggttcatg agcggcgacc tcggccgctt cgacgccggg ggcaacctgc
     1021 aaatcgtggg gcgcaagaag gacctcatca ttcgcggcgg ctacaacatc cacccggcgc
     1081 gcatcgagaa cctggcgctg aagcatcccg ccgtgctgaa ggccgccgcc ttcccggtgc
     1141 ccgacgagcg gctgggcgag cgcgcctgcc tcgccatcat cgcgcgcgac ggcacgcgcg
     1201 tggacgggga cgagttgctg cgtcacctcg acaccgcggg cttgtccaag tacgacatgc
     1261 cggagtactt catagcgctc gacgcgtttc ccctgacggc gagcggcaag atcctcaagc
     1321 gcgagctcgt cgagtgggcc aagagcggca ggatccgccc cgcgcccgtg cgctggacaa
     1381 catcggaacg gaaaaaggcg tgaatcgtga acggtgaacg gatttaaccc tgctgttgcg
     1441 acgctttacc attcatcatt caccattcac cattcaccat tcacgaatca cggtttatat
     1501 atggcgatcg agctcacgcg tgtcgaggaa ttcgcgctca tcaccctcaa ccggcccgaa
     1561 gcgctcaacg cgctttcgtt cgcgctgatc gcggagctcg cccgcgcctt cgatgaggtc
     1621 gcggcgagta acgcccgcgc gctgctcatc accggggcgg gggccaaggc cttctgcgcg
     1681 ggcgccgaca tcaaggaact caccggccgc ccgctcgcgg accagaagcg cggcgcagag
     1741 ctgggtcagg cgacctttgc caagctcgac cggttgccgg tcccatcggt cgccatcgtg
     1801 aacggctacg cgttcggcgg cgggctggaa ctcgccctcg cgtgcacgtt ccggctcgcc
     1861 acgcgcaacg cgaagatggg cctccctgaa atcaagctgg gactgattcc cgg
//
LOCUS       DBNGWZ010000583         2898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1598971, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000583 DBNGWZ010000000
VERSION     DBNGWZ010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1598971"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="AC16CX_18265"
     CDS             complement(<1..104)
                     /locus_tag="AC16CX_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3014488.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAMTM"
     gene            complement(231..2348)
                     /gene="fusA"
                     /locus_tag="AC16CX_18270"
     CDS             complement(231..2348)
                     /gene="fusA"
                     /locus_tag="AC16CX_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3014489.1"
                     /translation="MPRKTPIERYRNIGITAHIDAGKTTTTERILFYTGVSHKLGEVH
                     DGTAIMDWMEQEQERGITITSAATTCFWKGMDKTYPEHRINIIDTPGHVDFTIEVERS
                     LRVLDGACTVLCAVGGVQPQTETVWRQANKYRVPRLIFVNKMDRTGADFKRVYEQVQT
                     RLKAHPVPIQLPIGSEEKFKGVVDLVRMKAIYWDDGTQGMKFEAAEVPAHLVDEARHW
                     REKMVESAAEASEELMNRYLEKGELSVEDIKKGLRARTIHGEIFPMLCGSAFKNKGVQ
                     AMLDAVIDYLPSPADVPPVKGIKENGKVDERTPNDDAPFSGLAFKIMTDPFVGQLTFF
                     RVYSGVVSTGDTVYNPIKGRKERLGRILQMHANDREEIKEVRAGDIAAAVGLRDYTTG
                     DTLTDPQKIITLEKMEFPEPVIHVAVEPKTKADQEKMGMALNRLAQEDPSFRVRTDEE
                     SGQTIISGMGELHLEIIVDRMKREFGVEANVGAPQVAYRETIKKAAEKVEGKFIKQTG
                     GRGQYGHVWLRLDPQPHGTGFEFVDAIKGGAVPREYIPAVKKGVQESLPNGVLAGFPV
                     VDVKVTLFDGSYHEVDSNENAFKMAGSFAFKEGLRRASPTLLEPIMSVEVETPDDFMG
                     NVVGDLNSRRGVIQGMEEIPGGGKAIRAEVPLAEMFGYSTTMRSLSQGRATYTMEFKH
                     YAEAPRQIAEAVINKYTGKEERK"
     gene            complement(2434..>2898)
                     /gene="rpsG"
                     /locus_tag="AC16CX_18275"
     CDS             complement(2434..>2898)
                     /gene="rpsG"
                     /locus_tag="AC16CX_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR3014490.1"
                     /translation="RRREIPKREVLPDPKYTSQDIAKFINVLMTRGKKSVAEAIVYGA
                     LDQIKKKTSKDPLEVFTQAINNVKPMVEVKSRRVGGANYQVPVEVRPVRRVALAMRWL
                     RDAARGRGEKSMGARLASELAEAAEGKGGAMKKREEVHRMAEANKAFSHYRF"
BASE COUNT          450 a          957 c          849 g          642 t
ORIGIN      
        1 atcatcgtca tcgccgccgt cagcgtcgtc ttgccatgat ccacgtgccc aatcgtgccc
       61 acgttcacgt gcggcttggt gcgctcaaat ttgccctttg ccatctcttt gctccactct
      121 ttgtgccttt aggccctctt gcccgcaatc tatcccgatc aaccaacctc gacgcgctga
      181 atccgcaaaa aaattatcga attccataaa gaagaaagga aacaggaatc ctatttcctt
      241 tcttctttgc ccgtgtactt gttgatcacc gcctccgcga tctgcctcgg tgcctcggcg
      301 taatgcttga attccatggt gtaggtcgca cgcccctggg agagcgagcg catcgtggtc
      361 gaatagccga acatctcggc gagcggcacc tcggcacgga tcgccttgcc gccccccgga
      421 atctcctcca tgccctggat cacgccgcgg cgcgagttga gatcgcccac cacgttgccc
      481 atgaaatcgt ccggcgtctc cacttccacc gacatgatcg gttcgagcag cgtggggctg
      541 gcgcggcgca ggccttcctt gaacgcgaac gagcccgcca tcttgaacgc attctcgttc
      601 gaatccacct cgtggtacga gccgtcgaac agcgttacct tgacgtccac caccgggaaa
      661 cccgccagca cgccgttggg cagcgattcc tgaacgccct ttttcaccgc cgggatgtat
      721 tcgcgcggca ccgcgccgcc cttgatcgcg tccacgaact cgaagcccgt gccatgaggc
      781 tggggatcga gcctcaacca gacgtggcca tactggccgc gcccgccggt ctgcttgatg
      841 aacttgcctt cgaccttttc ggccgccttc ttgatggttt cgcgataggc aacctgcggc
      901 gcgccgacgt tggcctccac gccgaattcg cgcttcatgc ggtcgacgat gatctccagg
      961 tgcagctcgc ccatgccgga gatgatcgtt tgcccggact cctcgtcggt gcgcacgcgg
     1021 aacgaggggt cctcctgcgc aaggcggttc agcgccatgc ccatcttttc ctggtccgct
     1081 ttggtcttcg gctcgaccgc gacgtggatc accggctcgg ggaattccat cttctcgagc
     1141 gtgatgatct tctgcgggtc ggtcagcgtg tcgccagtgg tgtagtccct caggcccacc
     1201 gccgcggcga tgtcgccggc gcggacctcc ttgatctcct cgcggtcgtt ggcatgcatc
     1261 tgcaatatgc ggccgaggcg ttccttgcgg cccttgatcg ggttgtagac cgtgtcaccc
     1321 gtgctcacga caccggaata aacgcggaag aaagtgagct ggccgacgaa ggggtcggtc
     1381 atgatcttga acgcgaggcc cgagaatggc gcgtcatcgt tcggcgtgcg ctcgtccacc
     1441 ttgccgtttt ccttgatgcc ctttaccgga ggaacgtccg cgggtgacgg caggtaatcg
     1501 atcacggcat cgagcatcgc ctgcacaccc ttgttcttga acgccgaacc gcacagcatc
     1561 gggaagatct cgccgtggat ggtgcgggcg cgcagcccct tcttgatgtc ctcgaccgag
     1621 agctcgccct tttcaaggta gcggttcatg agctcctccg aagcctcggc ggccgattcc
     1681 accatcttct cgcgccagtg cctggcctcg tctacgaggt gggccgggac ttcggcggcc
     1741 tcgaacttca tgccctgcgt gccatcgtcc cagtagatag ccttcatcct gacgaggtcc
     1801 actacgccct tgaatttttc ctccgatccg attggcagct ggatcggcac cgggtgggcc
     1861 ttgagccggg tctgcacctg ctcgtagacc cgcttgaagt cggcgccggt gcggtccatc
     1921 ttgttgacga aaatcagccg cggcacgcga tacttgttgg cctggcgcca gaccgtctcg
     1981 gtctgcggct gcacgcctcc taccgcgcac aacacggtac acgcgccgtc gagcacgcgc
     2041 agcgagcgtt cgacctcaat ggtgaaatcc acgtggcccg gcgtgtcgat gatgttgatg
     2101 cggtgctcgg gataggtctt gtccatgccc ttccagaagc aggtcgtcgc cgccgaggtg
     2161 atggtaatgc cgcgctcctg ttcctgctcc atccagtcca tgatcgcggt gccgtcgtgc
     2221 acttccccca gcttgtgcga gacgccggtg tagaacagga tgcgctcagt ggtcgtggtc
     2281 ttgccggcat cgatgtgggc ggtgatgccg atgttgcggt agcgctcgat gggggtcttg
     2341 cgtggcattg tcgaagcctt gcctgtcacc gcggaggtgg gggagcgatg agtaagcaca
     2401 ttgtccattc tctcggcgtc tctgcggttc aatctaaaac cggtaatgcg agaaggcctt
     2461 gttggcttcc gccatgcggt gcacttcctc gcgcttcttc atcgcgccgc ccttgccctc
     2521 ggcggcttcg gccagttcgc tggcgagccg cgcgcccatg gatttctcgc cgcggccccg
     2581 cgccgcgtcg cgcagccagc gcatggcgag cgcaacccgc cgtaccggcc gcacttcgac
     2641 cggcacctgg tagttggcgc cgccgacgcg gcggctcttc acttcgacca tcggcttcac
     2701 gttgttgatc gcctgcgtga acacctccag cgggtctttg ctggtcttct tcttgatctg
     2761 gtcgagcgca ccgtagacga tcgcctccgc gaccgacttc ttgccgcggg tcatgaggac
     2821 attgatgaac ttggcgatgt cctggctcgt atacttcggg tcgggcagga cctcgcgctt
     2881 cggaatctct ctgcgtcg
//
LOCUS       DBNGWZ010000584         2652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_213489, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000584 DBNGWZ010000000
VERSION     DBNGWZ010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_213489"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="AC16CX_18280"
     CDS             <1..1247
                     /locus_tag="AC16CX_18280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3014491.1"
                     /translation="LHGEWMDTGDYGYVAEGEIHITGRVKDVIIRAGRNIHPYELEEA
                     VGGVEGIRKGCVAVFGSRDERSGTERVVVLAETRETDPARREALHGRINDLAVALINM
                     PADDIMLAPPGTVLKTSSGKIRRAASREVYEGGGRPGTRAVWLQVVRLTWSAILPQVR
                     RSLRAAAGLAYGAYALFLLGTLGALNWTACALMPRAEWAWKLSHRVARAFLWLTGMPY
                     TVRGLEHLPAGRPFMLVVNHGSYLDGPAVIGALEKTNCFVAKRELLDHWVPRIYLKSV
                     GSVFVDRLDAQRSVEDTGRFVEAARGGRSLIVFPEGTFRRMPGLLPFRMGAFVVAAQA
                     GVPVVPMTIRGTRSALRDGQWLFHRGRISVTFSAPIEPVGTDWSAAIQLKDAARAEIL
                     RLCGEPDLGEETSLPPKRPPKQ"
     gene            complement(1379..2482)
                     /locus_tag="AC16CX_18285"
     CDS             complement(1379..2482)
                     /locus_tag="AC16CX_18285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3014492.1"
                     /translation="MALKRPKGHSGTLLVLEHTSSILKDNPLGDPHVRKLAVWLPPQY
                     DQGGKPRFPVLYDLVGFTGSGLAHTNWKPFGDNVPERVARLIRQQKMGPAIIVFPDCF
                     TAFGGNQYVNSSAIGNYADYLTREIIPFVDSEFRTLAAREHRGCFGKSSGGYGAILHA
                     MKYAKYWGAIADHSGDAYFDFVYWHDWPNTLNELAKHRLPRRRAGKYDARRESGRKGL
                     AEGLDDGRIKRFLKHVWKKEKLSTAEGHCIMNLCMAATYDPDPGAPPGFRVPFNLESG
                     EFIARRWNRWREHDPINLVAKYRDNLKSLRGIYIDCGWRDQYHIHYGARILSQRLALA
                     GIRHAYEEFDDNHSDIDYRMDVSLPFLYKALKP"
BASE COUNT          429 a          854 c          901 g          468 t
ORIGIN      
        1 ttctgcacgg cgagtggatg gacaccggcg actacggcta cgtcgccgag ggcgagatcc
       61 acatcaccgg gcgcgtcaag gacgtgatca tccgcgccgg ccgcaacatc cacccctacg
      121 agctcgaaga ggcggtcggc ggagtggaag gcatccgcaa gggttgcgtc gccgtcttcg
      181 gcagccgcga cgagcgctca ggcaccgagc gggtcgtcgt gctcgcggaa acgcgcgaga
      241 ccgatcctgc ccgccgtgag gcgctccacg gcaggatcaa cgatctcgcc gtggcgctga
      301 tcaacatgcc ggcggacgac atcatgctgg cgccgccggg caccgtgctc aagacctcga
      361 gcggcaagat ccgccgcgcc gcgagccgcg aggtgtacga gggcggcggc cgccccggca
      421 cgcgcgccgt gtggctgcag gtcgtgcgcc tgacctggtc ggcaatcctg ccgcaggtgc
      481 ggcgctccct gcgcgccgcc gccggcctgg cgtacggcgc ctacgcgctg ttcctgctcg
      541 gcacgctggg cgcgctcaac tggaccgcct gcgccctcat gccgcgcgct gaatgggcgt
      601 ggaagctgag ccaccgcgtc gcgcgcgcct tcctgtggct gacgggaatg ccctacaccg
      661 tgcgcgggct cgagcacctc ccggcgggac ggcccttcat gctagtcgtc aaccacggga
      721 gctatctcga cggcccggct gtgatcggcg cgctggaaaa aaccaattgc ttcgtcgcca
      781 agcgcgagct gctcgatcac tgggtgccgc gcatttatct caagtccgtc ggcagcgtct
      841 tcgtggaccg cctcgacgcg cagcgcagcg tggaggacac cggccgcttc gtggaagcgg
      901 cgcgcggcgg gcgctcgctc atcgtcttcc ccgaaggcac gttccgccgc atgccggggt
      961 tactgccgtt ccggatggga gcgttcgtgg tcgcggcgca ggcgggcgtg ccggttgtgc
     1021 cgatgaccat ccgcggcacg cgatccgcgc tgcgcgacgg acagtggttg ttccaccgcg
     1081 gccgcatcag cgtaaccttc agcgcgccga tcgagccggt tggcaccgac tggagcgcgg
     1141 cgatccaact gaaggacgcg gcaagggcgg aaattctgcg gctgtgcggc gagccggact
     1201 tgggcgagga gacatcgctg ccgcccaagc ggccaccaaa gcagtgacga ctgacggcac
     1261 gtcaagttgg caattggcgg tcatcagccg gcagtaagtg caatgatctt gctgcaaact
     1321 gcgaactgcc tactgcaagc tgctattccc tccgccctca ttcctcatcc ttaatccttt
     1381 acggtttcag cgccttgtag agaaacggca agctcacgtc catccggtaa tcgatatcgg
     1441 agtggttgtc gtcgaactcc tcgtaggcgt gccggatgcc ggccagagcg agtcgctgcg
     1501 acaggatgcg cgcgccgtaa tggatgtggt actggtcgcg ccagccgcaa tcgatgtaga
     1561 tgccgcgcag cgattttaag ttgtcccgat acttcgcgac gaggttgatc gggtcgtgct
     1621 cgcgccatcg gttccagcgc ctcgcgatga actcgcccga ttcgaggttg aacgggacgc
     1681 gaaagccggg cggagccccc ggatccgggt cgtaggtggc ggccatgcag aggttcatga
     1741 tgcaatggcc ttctgcggtc gagagctttt ccttcttcca aacgtgcttg aggaagcgct
     1801 tgatgcgccc gtcgtcgagc ccttcggcga ggcctttgcg gcccgattcg cggcgcgcgt
     1861 cgtacttgcc ggcccgccgt ctcggcagcc ggtgcttcgc gagctcgttc agcgtgttcg
     1921 gccagtcgtg ccagtagacg aaatcgaagt acgcgtcgcc ggaatgatcg gcgatcgcgc
     1981 cccagtattt cgcgtacttc atcgcgtgga ggatggcgcc gtagccgccc gatgattttc
     2041 cgaagcagcc gcgatgctcg cgggcggcga gcgtgcggaa ctcgctgtcg acgaagggaa
     2101 tgatctcgcg cgtgagatag tccgcgtagt tcccgatcgc ggaggagttc acgtactggt
     2161 tgccgccgaa ggccgtgaag cagtccggaa aaacgatgat cgccggcccc atcttctgct
     2221 ggcggatgag ccgcgccacg cgctccggaa cgttgtcgcc gaacggcttc cagttggtgt
     2281 gcgcgagacc ggacccggtg aagccgacca ggtcgtagag cacggggaag cggggcttgc
     2341 cgccctggtc atactgcggc ggcagccaca ccgcgagttt gcggacgtgg ggatcgccga
     2401 ggggattgtc cttgaggatg ctcgacgtgt gctcgaggac gagcagcgtg ccggagtggc
     2461 ctttggggcg tttgagcgcc atggatcacc cgctggctat cgttacacga tgcctgggtt
     2521 cactgacaga gcgcgatggc gtcgccacct ccataagagg ccgtcgtcga ggttcatggc
     2581 ggtccgcatt cgatgtcccc ggcaggatgc cccaatatta gcattgcccg ataccgccct
     2641 tcccggccga cg
//
LOCUS       DBNGWZ010000585         3758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_213987, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000585 DBNGWZ010000000
VERSION     DBNGWZ010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_213987"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC16CX_18290"
     CDS             complement(<1..292)
                     /locus_tag="AC16CX_18290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014493.1"
                     /translation="MAQKTTVKLIPPPDPNPVKPKYTPPPGACDGHCHVFGPVKRFPL
                     AKERTYEPPDSPKENLAALHKHLGFERAVLVQASCHGTDNSAMLDAIKTSKGA"
     gene            459..911
                     /locus_tag="AC16CX_18295"
     CDS             459..911
                     /locus_tag="AC16CX_18295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3014494.1"
                     /translation="MTRPGKRRKPAKKVLGVGPVAAREVLRSGMPRALGVRLVSLTKK
                     RVVAEMRVKRMHINRSGRVNGGALMAFADVMGAAGTVANLPSGYRTGTLESKSNFFRS
                     GEGPLLRAVSVPLHIGRTTMVWQTTIRNRDGAKVAVVTQTQIVIPAES"
     gene            934..1743
                     /locus_tag="AC16CX_18300"
     CDS             934..1743
                     /locus_tag="AC16CX_18300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014495.1"
                     /translation="MVASSPAPATETREMMAAAKKVALVTGGASGIGRSAVLALARAG
                     YDVAINYRRSAAAAKKTAREAGELGAATLLCPCDVSDDQAVREMLQAVGRRFARLDVL
                     INNAGTTTDVKPQEIDLMTAAEWDRVFAVNVRGLFQVTRAALPLLRKAEHPCIVNTAS
                     IVGLRPGPQPLPYAASKAAVVNLTKTLAYNLGPQIRVNAVAPGWMEGDWMKWMLKERY
                     DELMARRAKLTPLKRCVTADDVAESMMSLIEGNRFVTGEVVVVDGGFSSST"
     gene            1840..3489
                     /locus_tag="AC16CX_18305"
     CDS             1840..3489
                     /locus_tag="AC16CX_18305"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008668 -
                     2,3-dihydroxybenzoate--[aryl-carrier protein] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HIR3014496.1"
                     /translation="MLEGVVPFPPAFADRYRERGYWADKSLAREFSVAFGEFADRAAL
                     FDGDRRFTYREIDRLSDRLALNLLALGLRPLDRVVVQLPNAAEFVILYFALQKLGAIP
                     VAALAAHRYSEVSQFVRVAGAVACVYPPAQGEFRFGPMVKRVQQESPHLQWRIVLGPA
                     APGEHSLRELIEREPALGRSALDVIAIDPVDPAVFQLSGGTTGIPKLIPRTHNDYAYN
                     SKTAAQVCGVDGDSVLLIALPIAHNLPLACPGVQGAFLRGAKVVLCPSTRPPDMFALI
                     ERHRVTHVKVVPALLIRLINSPEITRFDLSSLKVIQSGGQHMQPEVRFRARRLMPGAF
                     VQENFGMAEGTLFFVRFGDPEDVQIETCGRPISPDDEVKLVDDEGREVAPGEVGELAC
                     RGPYTLRGYYRVPEYNARAFTTDGFYLTGDLMRLHPSGNYVVEGRKKDLINRGGEKIS
                     AEEIENLILSHPAVQNVACVAMPDPELGERMCACLILRTGHSLALEELVGFLKTKEIA
                     KYKLPERLEVMGEFPLSAFGKVSKNALAERVKRKLEQDRAA"
     gene            3511..>3758
                     /locus_tag="AC16CX_18310"
     CDS             3511..>3758
                     /locus_tag="AC16CX_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014497.1"
                     /translation="MRIIDLHCYPGTKEWIACQGPYVEALGRYWKREWKAKTEDEVIK
                     DFAGAGVEALLVALDLETTLGTPPCSNAYVHAMWKRHP"
BASE COUNT          634 a         1209 c         1298 g          617 t
ORIGIN      
        1 aggcgccttt cgaagtcttg atcgcatcga gcatcgcgct gttgtcggtg ccgtggcagc
       61 tcgcctgcac cagcacggcg cgctcgaacc cgagatgctt gtgcagcgca gccaggtttt
      121 ccttgggcga gtccggcggc tcgtaggtcc gctccttcgc gagcgggaag cgcttcaccg
      181 gcccgaagac gtggcagtga ccgtcgcacg cgccgggcgg cggcgtgtac ttgggcttca
      241 ccggattggg atcgggcggc ggaatgagct tgacggtggt tttttgagcc atgacgtgaa
      301 ctcccgggtt cgcggcgggc cggcgtcgta tctgccgcga agttaacgca gtgacggggt
      361 cgggtcaacc accgcgccga cgtgcctcgc gttgccagca gggggagcag gacggataat
      421 ccgctgccga caaggcgtga cgtgatgggg cgtgactcgt gactcgaccg ggcaagaggc
      481 gcaagcctgc gaagaaagtg ctgggagtag ggccggttgc ggcccgggag gtgctgagaa
      541 gcgggatgcc gcgcgcgctc ggcgtgcgcc tcgtttcgct cacgaagaag agagtcgtgg
      601 ccgagatgcg ggtgaagcgc atgcacatca accgctcggg gcgcgtgaac ggcggcgccc
      661 tcatggcgtt cgcggacgtg atgggcgcgg ccggcaccgt cgcgaacctc ccgtccggct
      721 accggaccgg cacgctcgag tcgaaatcca acttcttccg gtccggcgag ggcccgctgc
      781 tgcgcgcggt gtccgtgccg ctgcacatcg gccgcaccac catggtgtgg cagaccacca
      841 tccggaaccg tgatggagcc aaggtcgcgg tcgtcaccca gacccagatc gtcattccag
      901 cggaatccta ggtctgcgag gtcgcttccg ctcatggtag catcctctcc cgcgccggca
      961 acggaaacgc gggagatgat ggcggcagcg aaaaaggtcg cgttggtgac gggcggggcg
     1021 agcggcatcg gccgcagcgc ggtgctggcg cttgcgcgcg cgggctacga cgtcgcgatc
     1081 aattatcgcc gcagcgccgc ggccgcgaag aagacggcgc gcgaggccgg cgagctcggc
     1141 gcggccacgt tgctttgtcc gtgcgacgtg agcgacgatc aggcggtgcg cgagatgcta
     1201 caggccgtgg gccggcgctt cgcccggctc gacgtgctga tcaacaacgc cggcaccacc
     1261 accgacgtca agccccagga aatcgacctc atgactgccg cggaatggga ccgcgtgttc
     1321 gcggtcaacg tgcgcggatt gttccaggtc acccgcgcgg cgctgcccct gctgcggaag
     1381 gcggagcatc cgtgcatcgt gaacacggcg agcattgtcg gccttcgtcc cgggccccag
     1441 ccgctgccgt acgccgcgag caaggcggcg gtcgtcaatc tcacgaagac tctcgcctac
     1501 aacctcggcc cgcagatccg ggtgaatgcc gtcgcgccgg gatggatgga aggcgactgg
     1561 atgaaatgga tgctgaagga gcgctacgac gagctgatgg cgcgccgtgc gaagttgacg
     1621 cccttgaagc gctgcgtgac ggcggacgat gtcgcggaaa gcatgatgag cctcatcgag
     1681 ggcaaccgct tcgtcaccgg ggaagtggtc gtggtggacg gcggcttcag cagctccaca
     1741 tgaaacacgg gagatcggca gggcgggagg gcgtgagggc gaaacgcttg gttgccttcg
     1801 ttccatctcc cgctctcccg cactcccgct ctcacggata tgcttgaagg tgttgttcct
     1861 ttcccgcccg cgttcgcgga ccgctatcgc gagcgcggat actgggcgga caaatccctc
     1921 gcccgggaat tttccgttgc attcggggaa ttcgcggacc gcgccgcgct gttcgacggc
     1981 gaccggcgtt tcacctaccg cgaaatcgac cggctgagcg accggctcgc gctcaatctt
     2041 cttgcgctcg gactgcggcc gctcgatcgc gtcgtggtcc agctgcccaa tgcagccgaa
     2101 ttcgtgatcc tgtacttcgc gctgcagaag ctcggcgcga taccggtcgc ggcgctcgcc
     2161 gcccatcgct acagcgaggt gagccagttc gtccgcgtcg ccggggccgt ggcgtgcgtt
     2221 tatccgccgg cgcagggcga attccggttc ggcccgatgg tgaagcgggt gcagcaggaa
     2281 tccccacacc tccaatggcg catcgtactc ggcccggccg cgcccggcga gcattcgctg
     2341 cgggaattga tcgagcgcga gccggcgctc ggccgctccg cgctcgatgt catcgccatc
     2401 gacccggtcg atcccgccgt gttccagctc tcgggcggga ccacgggcat ccccaagctg
     2461 ataccgcgca cgcacaacga ctacgcctac aactcgaaaa ccgcggcgca ggtgtgcggc
     2521 gtggacggcg attcggtgct cctgatcgcg ctgccgatcg cccacaacct gcccctcgcg
     2581 tgcccgggcg tgcagggcgc cttcctgcgc ggggcgaaag tggtgctgtg ccccagcacg
     2641 cgcccgcccg acatgttcgc gctgatcgaa cggcatcgcg tcacccacgt caaggtggtg
     2701 ccggctctgc tgatccggct catcaacagc cccgagatca cgcggtttga cctttcctcg
     2761 ctcaaggtca tacaaagcgg tggccagcac atgcaacccg aggttcgctt ccgcgcaagg
     2821 cgactgatgc cgggcgcgtt cgtgcaggag aatttcggca tggcggaagg cacgctcttc
     2881 ttcgtgcgct tcggcgatcc cgaggacgtg cagatcgaga cctgcggcag gccgatatcg
     2941 ccggacgacg aagtgaagct cgttgatgac gagggccgcg aggtcgcgcc gggggaggtg
     3001 ggggagctcg cgtgccgcgg cccgtacacg ctgcgcgggt actatcgcgt gcccgaatac
     3061 aacgcccggg cattcaccac ggacgggttc tatctcaccg gcgacctcat gcggctccac
     3121 ccttcgggca attacgtcgt cgaggggcgc aagaaggatc tcatcaaccg cggcggggaa
     3181 aagatcagcg cggaggagat cgagaacctg atcctctcgc atcccgcggt gcagaacgtc
     3241 gcgtgcgtgg cgatgcctga tccggagctg ggtgaaagga tgtgcgcctg cttgatactt
     3301 cgcaccggcc actcgctcgc gctcgaggag ctggtcgggt tcctgaagac gaaggagatc
     3361 gcgaagtaca agctcccgga gcggctcgag gtcatgggcg aattccccct ctccgccttc
     3421 ggcaaggttt cgaagaacgc gctcgcggag cgggtgaaac gcaagctcga gcaggaccgg
     3481 gccgcctgag cgccgcatcg aggacgacga atgcgcatca tcgacctgca ctgctacccc
     3541 ggcacgaagg aatggatcgc gtgccagggg ccctacgtcg aggcgctcgg ccgctactgg
     3601 aaacgcgagt ggaaggcgaa aaccgaggat gaggtcatca aggacttcgc cggcgcgggg
     3661 gtcgaagcgc ttctggttgc gctcgatctc gagacgaccc tgggcacgcc gccgtgctcc
     3721 aacgcctatg ttcacgcgat gtggaagcgc catccgga
//
LOCUS       DBNGWZ010000586         3047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1219405, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000586 DBNGWZ010000000
VERSION     DBNGWZ010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1219405"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2291)
                     /locus_tag="AC16CX_18315"
                     /pseudo
     CDS             complement(1..2291)
                     /locus_tag="AC16CX_18315"
                     /inference="COORDINATES: protein motif:HMM:NF005544.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
     gene            complement(2947..3047)
                     /locus_tag="AC16CX_18320"
                     /pseudo
     CDS             complement(2947..3047)
                     /locus_tag="AC16CX_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
BASE COUNT          508 a          947 c          955 g          637 t
ORIGIN      
        1 ttctggtagg tcggctcgat gcactgcgac agccccgtga tgttggcgat cgtcgcggtg
       61 ggcgcgatcg cgaggcagtt cgagttccgc atgccgactt gctggatgcg ccttttcagg
      121 ccctcccagt cgagcagggc ggacatgtcg cactcgacgt agccgccgcg ttcctcggcg
      181 agcagcttca aggtgtcgca cggcaggacg ccgcggtccc acagcgagcc cttgaaggtc
      241 gagtagggcc cccgctcctc cgcgaggtcg gtcgaggcct ggtacgcggt gaacgcgatc
      301 gcctccatcg agcggtcggc gaacgtcacc gccgcctgcg agccgtacgg gatgcgcagc
      361 atgtgcaggc agtcctggaa acccatgatg ccgagcccga ccggccggtg cttgaagttg
      421 gagttgcgcg ccttcccgac cgagtagaag ttcaagtcga tgacgttgtc gagcatgcgc
      481 atcgccgtgc cgacggtgcg gcgcagcttt tccatgtcca tgcggccggt ggagacatcc
      541 aggtgcgccg gcatgttgac cgagcccagg ttgcacaccg cgatctcgtc ggggccggtg
      601 ttcagcgtga tctcggtgca gaggttggag ctgtgcacca cgcccacgtg gctctgcggg
      661 ctcctcaggt tgcacggatc cttgaaggtg agccagggat acccggtctc gaacagcatg
      721 ctgagcatct tgcgccacag ctgcgtcgcc ggaatcttct tgaagagctt catctcgccg
      781 cgcgcgcact tcgcctcgta ctcgagatag gcctgctcga agctcttccc gaacttgtcg
      841 tgcaggtccg gcacgtcgga gggcgagaac agcgtccact cgccgccctc catcacgcgc
      901 ttcatgaaga gatccggcac ccagttggcg gtattcatgt cgtgggtgcg gcggcggtca
      961 tcgccggtgt tcttgcgcag ctccaggaat tcctcgatgt cgaggtgcca ggtctcgaga
     1021 tacgaacaca ccgcgccctt gcgcttgccg ccctggttca ccgccacggc ggtgtcgttc
     1081 accaccttga gaaacggtac gacgccctgg ctcttgccgt tggtgccctt gatgtgggag
     1141 ccgagcgcgc gcaccggcgt ccagtcgttg ccgagcccgc cggcgaactt ctgcagcaac
     1201 gcgttttcct tgatcgcctc gtagatgccg tcgagatcgt cggaaacggt ggtgagatag
     1261 cagctcgcga gctgcgagcg gcgcgtgccg gagttgaaca gcgtcggcgt cgagctcatg
     1321 aagtcgaagc tcgagagcac gttgtagaac tcgatcgcgc gcgcctcgcg ctggtcctcg
     1381 ggctcgttca acgcgagccc catcgccacg cgcatgaaga agatctgcgg cagctcgatg
     1441 cggcggccgc gcacgtgcag gaaataacgg tcgtagagga tttgcagccc gaggtaggtg
     1501 aacttgaggt cgcggttggc gtcgagcgcc gccccaaggc acttcaagtc gtagtgggcg
     1561 aggcgctcgt cgagcagctc cgcctcgatc cccttcttca cgaactccgg caggtactcg
     1621 gcgtaacggg cgtgcatctc cgcctgcgtc acttccacgc ccagcgtctc gagccgcagc
     1681 gtgtgcagca gcaggcgcgc ggtgacgtag ctatagccgg ggtcctgctc gatgagcgcc
     1741 cgcgcggcca gcacgagcga cttcctcact tcctccatcg gcaccccgtc gtagagatcg
     1801 cgctgcatcg cctgcaggat cggctccgcg ctcacctcgc gcccgagccc ctcgcacgcg
     1861 tcccgcacca gcgccgtgat tctggcgcgg tcaagcggct tcaactgccc gccctctttc
     1921 acgttgatcg ccggctgctg cgcgcgcttc tccttctcct gcttctcgcg ctcccgggcg
     1981 cgctccctcg cccgttcctc gcggtagagc acgtaggcgc gcgcgacgtt gtgctcgccg
     2041 gcgcgcatga gcgcgagttc cacctggtcc tggatgtcct cgatgtggaa ggtgccgccg
     2101 gagggctggc ggcgcagcag cgcacccacc acgttttcgg tgaggatcgc cacctgctcg
     2161 cgcacgcgcg cggacgctgc gccggtgccg ccgttgaccg cgatgaatgc cttcgtcatc
     2221 gcgatcgaga ttttcgcggg ctcgaagccc atgacggcgc cattgcggcg gatgatcttg
     2281 tactgggcgt agcgtgcctc acgtgcggta ggcgtttcat tcagggcaag gttggcggcg
     2341 ggagtgatta gggctaattc ggtagcgacc tgcattttgt aactccttga gtttgaagtt
     2401 cttttaactc aaggggaggt tctcctcccc ccttccaaaa tctctctact gtgctcaacc
     2461 acaatatctt gtggtcgaat cttgatttag atcaaataat agtagtctga tttttcgaaa
     2521 gcaataggct tcatgaactt tttcttaaat tctcgattta tcgaaaaaaa ccgcgggcaa
     2581 actgatgaaa aagcgggcaa aactgctcac aaattgagca gttgcccgct ttgccaggaa
     2641 tggtggcctg aagccggttt aagggctgtg gataaacagg gctaaaacgg acgtgaaacg
     2701 ccgctttctc cgcgctttgg aacggacgaa ggactcgccg ctcaacgtgc gggaacgtgg
     2761 attttcagag ggaaaagtga ctctcgctcg aacaatgcct ccctattttt tcttcgcttt
     2821 gtctcggcgt cttggcgttt tggcggttcc agttgaggga tgagggatga gggatgaggg
     2881 atgagggttt aaccctactt tctcttgtgt ccttcgtgtc cttcgtggtt cagctttgtc
     2941 ctttaacatg gcgcggtagc gcgcccagtc gaactgcggc cccgggtcgg acttccgtcc
     3001 cggcgcgacg tccgagtgcc ccacgatgtc ggcgatcgga aaacgcc
//
LOCUS       DBNGWZ010000587         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_842365, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000587 DBNGWZ010000000
VERSION     DBNGWZ010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_842365"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC16CX_18325"
     CDS             <1..313
                     /locus_tag="AC16CX_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014498.1"
                     /translation="GVCKLGQSDPSHEPIHVFLAEAGRVNCQLMEEALRRKRGHFAVA
                     AHSGPSTELLERLRERPPDVAVISTNLSDGPMAGFSVLLLHVAPIPAQRSGTPFFVAS
                     P"
     gene            complement(473..1234)
                     /locus_tag="AC16CX_18330"
     CDS             complement(473..1234)
                     /locus_tag="AC16CX_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014499.1"
                     /translation="MEYAQARMQARLGARPGAVLWRELDGIRDLGAYLDAARGTPLRR
                     WIAGVDAGGNLHQIDMRLRERYRDHVREVARWMPQAWWPAVLWLLQLIDLPALVHLLS
                     GRAPFSWMFKEPGLRVLIKDGGGQGDAAVPVFLRSERNRRQGHAPDLDRARSAWLAEW
                     ERRWPAGDEETMLAMKRIVAAFSLHLERFRFGGMRDSWRLRARLEHELRILFRRFALR
                     PEACFAYLGLIALDLERLRAQLVRRALWRQEGVAA"
     gene            complement(1240..1581)
                     /locus_tag="AC16CX_18335"
     CDS             complement(1240..1581)
                     /locus_tag="AC16CX_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014500.1"
                     /translation="MRTSQVEEAEVEQAIARVLQAEREAQNAVTRCASECAELVAAAH
                     RHAVEVGARTERRIARWRENMGKMVERRVGELDSAAEAALCAEASDDGASGRLERAAA
                     AVARELIGAAK"
     gene            complement(1740..1997)
                     /locus_tag="AC16CX_18340"
     CDS             complement(1740..1997)
                     /locus_tag="AC16CX_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014501.1"
                     /translation="MNAPKFWMIIPALLVAGAVGLAFAKLPQPPMDDAAKAKAVEAKA
                     KAAEAAKKDAEQLAKAQDRVVERYNREKGVKTAASAPPKKQ"
BASE COUNT          336 a          748 c          618 g          363 t
ORIGIN      
        1 gggggtatgc aaattggggc aaagcgaccc gagccatgag cccattcacg ttttccttgc
       61 cgaagccgga cgagtaaact gccagctcat ggaagaagcg ctacgccgaa agcgcggcca
      121 ttttgcggtc gcggcccatt ccggcccctc gacagagttg ctcgagagat tgagagagcg
      181 gccgcccgat gttgccgtga tcagtacgaa tctctccgat ggacccatgg ccggcttctc
      241 cgttctgctg ctccacgttg cgccgatccc tgctcaacgc tcaggcacgc ccttcttcgt
      301 cgcatcccca taaatggggc gcgtcgaggg gcggaagagg ggtccctatg gggatctgac
      361 cccggagacg cgcatgggat cggcatcaac atgacgcgcc ctcgatcagc ttcacggttg
      421 catcccgcaa gcgggtccct gctccacgtt ccgccgatcc ctactccgcc gctcatgccg
      481 ctaccccttc ctgccgccac agcgcccgcc gcacgagctg cgcgcgcaaa cgctccagat
      541 cgagcgcgat caggcccagg tacgcgaagc atgcttccgg tcgcagcgcg aaccgccgga
      601 acagaatgcg cagctcgtgc tcgagcctgg cccgaagacg ccagctgtca cgcataccgc
      661 cgaaccggaa gcgctcgagg tgcagggaaa acgccgcgac gatccgtttc atcgcgagca
      721 tcgtctcctc atcgccagcg ggccagcgcc gttcccactc ggcgagccag gcggaacgtg
      781 cccgatcaag gtccggcgcg tggccttggc gcctgttccg ttcagaccgc aggaaaacag
      841 gaacggccgc gtccccttgt ccgccaccat ccttgatcag cacccgcaac cccggttcct
      901 tgaacatcca ggaaaacggg gcgcggcccg acaacaggtg gacgagcgcc ggcaggtcga
      961 tcagctgcag cagccagagc acggccggcc accatgcctg gggcatccac cgagcgactt
     1021 cacgcacatg gtcgcggtat ctttcgcgca gccgcatatc gatctgatgc agattgccgc
     1081 ccgcgtcaac gccggcgatc cagcgccgca gcggcgtgcc ccgggcggca tcgagatagg
     1141 ctccgaggtc gcggatgccg tcgagctcgc gccacagcac tgcacccggc cgcgcgccaa
     1201 gccgggcctg catgcgcgcc tgggcgtatt ccatggagct catttcgcgg cgccgatgag
     1261 ctccctcgcc acggctgcgg cggctcgctc cagccgaccg ctcgcgccgt catcggaagc
     1321 ctctgcgcac agggccgcct cggcggcgct gtcgagctcc ccgacccgcc gctcgaccat
     1381 cttgcccatg ttttcccgcc accttgcgat gcggcgctcg gtacgcgcgc cgacttccac
     1441 ggcgtggcga tgcgccgctg cgacgagctc cgcgcactca gatgcgcagc gcgtgaccgc
     1501 attctgggcc tcgcgctcag cctgcagcac gcgcgcgatc gcctgttcga cttctgcttc
     1561 ttcgacctgg ctcgtccgca taggtatcaa attccccgtc tgattccgcg ccagggcgcc
     1621 ggcgcgggcc atacttgcac acgcctaagt taccgtctct gtgcggcagg aatttgatat
     1681 ggatcaaagc attaaaaaac ccggccgcgg ccgggtttgt taaacgggaa tcccttcggt
     1741 tactgcttct tcgggggagc ggatgcagcg gtcttgaccc ccttctccct attgtagcgc
     1801 tccaccacgc gatcctgggc cttcgcgagc tgctcggcat ccttctttgc cgcctctgcg
     1861 gccttggcct tggcctccac cgccttggcc ttggccgcgt catccatagg cggttgaggc
     1921 agcttggcga aggcgagtcc caccgcgccc gctacgagca gcgccgggat gatcatccag
     1981 aattttggtg cgttcatagc ttccacccct ctttcatggc cgatgcgtgg gccggcgacg
     2041 gcaccccgcc cgcggccttg ccctg
//
LOCUS       DBNGWZ010000588         1683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1097200, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000588 DBNGWZ010000000
VERSION     DBNGWZ010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1097200"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="ybeY"
                     /locus_tag="AC16CX_18345"
     CDS             <1..300
                     /gene="ybeY"
                     /locus_tag="AC16CX_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018437711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3014502.1"
                     /translation="RALNRHFRGKDHATNVLTFVMRSQPRLEGDLALCAPVIAREAHS
                     QRKSTAAHYAHLVVHGMLHLQGYDHETEREARAMERRESRIVTRLGFPDPYGDQG"
     gene            394..1221
                     /locus_tag="AC16CX_18350"
     CDS             394..1221
                     /locus_tag="AC16CX_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR3014503.1"
                     /translation="MANDQDKKHSLLERIGAWLARAPEDREELIELLRAAYERNLIDA
                     DALAMIEGVLQVSEMQARDIMVPRAQMDVIDIHESPDQFIPMVIQTGHSRFPVIGENK
                     DDVIGILLAKDLLRHYAGEEEFNVREMLRPAVFIPGSKPLNVLLKEFRKNRNHMAIVV
                     DEYGGVAGLVSIEDVIEQIVGDIEDEYDFEDVEDNIVPEKGGQYRVKALTEIADFNAA
                     FGTRYSEEEFDTIGGLVLSRFGRVPKRGEQISFDNLALKVLRADSRRLHLLQVTKKK"
     gene            1245..>1683
                     /locus_tag="AC16CX_18355"
     CDS             1245..>1683
                     /locus_tag="AC16CX_18355"
                     /inference="COORDINATES: protein motif:HMM:NF039484.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014504.1"
                     /translation="MKETTGDKPELPSRFSIIFHLSSFVTAFLLGAVTVAGYAPFHLF
                     PVPVLTLAALFHLWSKAGSARAAAIIGFAYGAGLFLCGVSWVYVSLHDYGATPAPLAA
                     VVTLLFCGFLALFPAAVGYACSRPALPHAARYGLLAPAAWTLAE"
BASE COUNT          309 a          515 c          551 g          308 t
ORIGIN      
        1 cgcgcgctca accgtcactt ccgcggcaag gatcacgcga ccaacgtgct gactttcgtg
       61 atgcgcagcc agccgcggct cgaaggcgat ctcgcgctgt gcgcgccggt gatcgctcgc
      121 gaggcgcatt cccaacgcaa gagcacggcc gcccattacg cgcacctggt ggttcacggc
      181 atgctgcatc ttcaaggcta cgatcacgag accgagcggg aagcgcgcgc gatggaaagg
      241 cgggaatcgc ggatcgtcac gcggctcggc ttcccggacc cgtatgggga tcaaggatga
      301 aggcgaaagg cgaaaggcga aaggcgaaag gcctgtgggc cggatggccg tttccttccg
      361 acttccgact tccgacttcc gacttgcagt cgaatggcta acgaccagga caagaagcac
      421 tcgctgctgg agcgcatcgg agcgtggctc gcgcgcgcgc ccgaggatcg ggaagagctg
      481 atcgagctgc tgcgcgccgc ctatgagcgc aacctgatcg acgccgatgc gctcgccatg
      541 atcgagggtg tattacaggt gtcggagatg caggcgcgcg acatcatggt gccgcgcgcg
      601 cagatggacg tcatcgacat ccacgagtcg cccgaccagt tcattcccat ggtgatccag
      661 accgggcatt cgcgtttccc cgtgatcggc gagaacaagg acgacgtgat cggcatcctg
      721 ctcgccaagg acctgctgcg ccattacgcc ggcgaggagg agttcaacgt gcgcgagatg
      781 ctgcgcccgg cggtattcat cccgggatcg aagccgctca acgtgctgct caaggagttc
      841 cgcaagaacc gcaaccatat ggcgatcgtg gtggacgagt atggcggggt ggcggggctg
      901 gtgtcgatcg aggacgtgat cgagcagatc gtcggcgata tcgaggacga gtacgacttc
      961 gaggacgtcg aggacaatat cgtgcccgag aagggagggc agtaccgcgt caaggcgctc
     1021 accgagatcg ccgacttcaa cgccgcgttc ggcacccgct acagcgaaga ggaattcgac
     1081 accataggcg ggctcgtcct gtcgcgcttc ggccgcgtgc ccaagcgcgg cgagcagatc
     1141 agtttcgata acctcgcact caaggtgctg cgcgccgaca gccggcggct gcacttgctg
     1201 caggtgacca agaaaaagta acgatgaacg cggaacgatg aacaatgaag gaaacgaccg
     1261 gagataagcc ggagctgcct tcccgttttt cgatcatctt tcatctctca tcgttcgtca
     1321 cggcatttct cctcggcgcg gtgaccgttg cgggttacgc gccgttccat cttttcccgg
     1381 tgccggtgtt aacactggcg gcgctgttcc atttgtggtc gaaagcgggg agcgcgcgcg
     1441 ccgcggcaat tatcggtttc gcttacgggg ccggcctgtt tctgtgcggc gtctcgtggg
     1501 tttacgtgag cctgcatgat tatggcgcca cgccggcgcc gctcgccgcc gtcgtaactc
     1561 tcctgttctg cgggtttctc gcgctgtttc ccgcggccgt cggttacgca tgctccaggc
     1621 ccgctctgcc gcatgccgca cgctacggcc tgctggcgcc ggcggcctgg acgctggcgg
     1681 aat
//
LOCUS       DBNGWZ010000589         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1098004, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000589 DBNGWZ010000000
VERSION     DBNGWZ010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1098004"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="AC16CX_18360"
     CDS             complement(<1..825)
                     /locus_tag="AC16CX_18360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014505.1"
                     /translation="MRMTSINTVLVALAALWACAALAADPGQPYPTRPIRLICPYVPG
                     GGADIFSRTVGQKLTEALGQTVVVDNRAGANGGIGTELVAKAAPDGYTLLLANSGPMT
                     VNPVLYKKVAYNPIRDFAPISQGSIYFYVLVVLPSFPASNLKDFIAQLKAKPGQMTYG
                     STGIGGGNHLSGELFNLMTGTHSIHVPYTGSAAALAALLGGQLNYMFDTVITSVPLIR
                     AGRVKAIGVTARKRASSLPDVPTLDELGLKGFELTQWQAIAAPAGTPKPIVDLLYRE"
     gene            974..2413
                     /gene="selA"
                     /locus_tag="AC16CX_18365"
     CDS             974..2413
                     /gene="selA"
                     /locus_tag="AC16CX_18365"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3014506.1"
                     /translation="MLNPAASARRAIPSLDRLLQLDAVAGLIARYGRPLVTEAARAEL
                     ARLRAALARSDSAFDESGFVSACAARLARETQPSLRPVFNLTGTVLHTNLGRALMPPE
                     AAQAVARAMTRPVNLEFDLDGGDRGERDQHVERWLTRLTGAEAALVVNNNAAAVYLVL
                     NTLALKKEVPVSRGELIEIGGAFRMPDIMARAGVKLVEVGTTNRTHLKDYAGAISPRT
                     AALMKVHTSNYAIQGFTAAVPEERLAALAHKHKLPLITDLGSGMLVDLEDYGLPHEPT
                     PREALADGADIVTFSGDKLLGGPQAGLIVGRTELIERIRRNPMKRALRVDKITLAALE
                     AVLRLYADPARLKAALPTLSLLTRPAAEIEAQAQRLLPAVQSALQGKARAEVVAVKSQ
                     IGSGSLPVDLIASHGIAVSIPGKRRNALAASTAAAFRALPVPVIGRVSEGAFIMDLRC
                     LEHEDEFLAQLVHLKIDANLDAKPQSRKG"
     gene            2543..>3354
                     /gene="selB"
                     /locus_tag="AC16CX_18370"
     CDS             2543..>3354
                     /gene="selB"
                     /locus_tag="AC16CX_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR3014507.1"
                     /translation="MIVATAGHIDHGKTLLVKTLTGVDTDRLPEEKARGISIDLGFAY
                     WPLESGAVIGFVDVPGHERFIRNMLAGVCGIDFALFVVAADDGVMPQTVEHLHILDLL
                     GIRRGIAVITKADRAAPERVETVRAEVTALLAPSRLAGIPVLAVSAVTGTGIDELRQR
                     LIAAAGAFTTRAAEDQHFRFAIDRAFTIAGSGTVVTGTVFNGTVAVGDRLVVSPKGTP
                     VRVRGAQMRGQPAQRAQAGQRCALNLTGADLETVARGDWLLHEAIHAPTQRI"
BASE COUNT          556 a         1150 c         1115 g          533 t
ORIGIN      
        1 ctcgcggtag agcaggtcca cgatcggctt cggcgtgcca gcgggggcgg caatcgcctg
       61 ccattgcgta agctcgaaac ctttcagccc cagctcgtcg agcgtgggca cgtccggcag
      121 cgaggaggcg cgcttgcgcg ccgtgacgcc gatcgccttc acccttccgg ccctgatcag
      181 cggcactgaa gtgatcacgg tgtcgaacat gtagttgagc tgcccaccga gcagggcggc
      241 aagcgccgca gcgcttcccg tgtagggcac gtggatcgaa tgggtgcccg tcatcaggtt
      301 gaacagctcc ccggaaagat ggttgccgcc gccaatgccg gtggagccgt aggtcatctg
      361 tcccggcttc gccttgagct gcgcgatgaa atccttgaga ttgctggcgg gaaaggacgg
      421 cagcaccacc agcacgtaga aatagatgga cccctgcgag atcggggcga aatccctgat
      481 cggattgtag gcgaccttct tgtacagcac cgggttgacc gtcatcgggc cgctgttggc
      541 gagaagcagt gtatagccgt ccggcgccgc cttcgccacc agctcggtgc cgatgccgcc
      601 gttggcgccg gcgcggttgt ccaccaccac cgtttgcccc agcgcctccg tcagcttctg
      661 gccgacggtg cgcgagaaga tgtcggcgcc gccgccgggg acgtagggac agatcaagcg
      721 gatcggccgc gtcgggtacg gctgccccgg gtcggctgcc agcgcggcac atgcccacaa
      781 ggcggccaag gcgaccagaa ccgtgttgat agatgtcatt ctcatggttt tcctcctgca
      841 gggaggcccg acaatagcat aggggctgaa cttgggctaa actccccgct ccgttttcag
      901 catcctgtca catgcgcacg ccgcgcaggc cagacccggt ccctggacac ggcgcgatca
      961 gctgcccgcc gccatgctga atcccgccgc ttccgcgcgt cgcgcgatcc cctcactcga
     1021 ccggctgctc cagctcgacg cggtcgccgg gctcatagcg cgctacggcc ggccgctggt
     1081 gaccgaggcg gcgcgcgcgg aactcgcgcg gctccgcgcc gccctcgccc gttcagactc
     1141 cgcgttcgac gaatccggtt tcgtgagcgc gtgcgcggcg cggctcgcgc gcgagacgca
     1201 gccgtcgctc aggcccgtat tcaatctcac cggcacggta ttgcacacga acctcgggcg
     1261 cgccctcatg ccgcccgaag cggcgcaggc ggtcgcgcgg gcgatgacgc ggcccgtcaa
     1321 cctcgagttc gacctggacg gcggggatcg cggcgagcgc gaccagcacg tcgagcgctg
     1381 gctgacacgg ctcaccggcg ccgaggcggc gctggtggtc aacaacaatg ccgccgcggt
     1441 ttacctcgtg ctgaacacgc tggcattgaa gaaggaggtg ccggtgtcgc gcggcgagct
     1501 gatcgagatc ggcggcgcct tccgcatgcc cgacatcatg gcgcgcgccg gcgtgaagct
     1561 ggtcgaggtc ggcaccacca accgcaccca cctcaaggac tatgccggtg cgatctctcc
     1621 gcgcaccgcg gcgctgatga aggtccatac cagcaactac gccatacagg gattcaccgc
     1681 ggcggtgccc gaggaacggc tcgccgcgct cgcgcacaag cacaagctgc cgctgatcac
     1741 cgatctcggc agcggcatgc tggtggacct cgaggactac ggcctgccgc acgagcccac
     1801 gccgcgcgag gcgctcgccg atggcgcgga tatcgtcacc ttcagcggcg acaagctgct
     1861 gggcggcccg caagccggat tgatcgtcgg ccgcacggag ttgatcgaac gcatccgcag
     1921 aaacccgatg aagcgcgcgc tgcgcgtgga caagattacg ctcgccgcgc tggaggctgt
     1981 gctcaggctt tacgctgacc ccgcgcgcct gaaggcggcg ctgcccacgc tcagcctgct
     2041 cacgcgtcca gccgccgaga tcgaggccca ggcgcaacgg ttgcttcccg ccgttcagtc
     2101 ggcgctgcag ggcaaagctc gtgccgaggt cgtcgcggtc aaaagccaga tcggcagcgg
     2161 ctcgcttccc gtggacctga tagcgagcca cgggatcgcc gtcagcatcc ccggcaagcg
     2221 gcgcaatgcg ctggcggcga gcaccgcggc ggcgttccgc gcgctgccgg tacccgtgat
     2281 cggccgggtc agcgagggcg ccttcatcat ggacctgcgc tgtctcgagc acgaggatga
     2341 gttcctcgcg cagctggttc acctgaaaat cgacgcaaac ctcgacgcga agccgcaaag
     2401 ccgcaaaggg tgatccaatt ggtcagaacc ttcgacgcag aaccacttgg ccgccaggag
     2461 tgaactcgat ttggtttggc ttttcattcc atgtcttctt tgcgactttg cgtctttgcg
     2521 tcaaagtcct tggggctaaa cagtgatcgt agcgaccgcg ggacacatcg accacggcaa
     2581 gacgctgctg gtgaagacgc tcaccggcgt ggacactgac cgcctgccgg aggaaaaggc
     2641 gcgcgggatc tcgatcgacc tcggcttcgc ctactggccg ctcgagagcg gcgccgtgat
     2701 cggcttcgtg gacgtgcccg ggcatgagcg tttcatccgc aacatgctcg cgggcgtgtg
     2761 cggcatcgac ttcgcgttgt tcgtggtcgc agccgacgac ggcgtcatgc cgcagaccgt
     2821 cgagcacctg cacatactcg atctccttgg catccgccgc ggcatcgccg tgatcaccaa
     2881 ggccgaccgc gccgcgcccg agcgcgtcga gacggtccgg gcggaggtaa ctgccttgct
     2941 cgcgcccagc cggctcgccg ggattcccgt gctcgccgtc tcggccgtga ccggcaccgg
     3001 catcgacgag ctcaggcagc ggctgattgc cgccgccggc gcattcacaa cgcgcgcggc
     3061 agaggaccag catttccggt tcgcgatcga ccgcgccttc accatcgctg gcagcggaac
     3121 agtggtcacg ggcaccgttt tcaacggcac cgtcgccgta ggcgaccgcc tggtggtctc
     3181 accgaagggc acgccggtgc gcgtgcgcgg agcgcagatg agggggcagc cggcgcagcg
     3241 ggcgcaggcc gggcagcgct gtgcattgaa tctcaccggc gccgatctgg aaaccgtcgc
     3301 gcgcggcgac tggctgcttc acgaggcgat ccacgcgccg acgcagcgga tcga
//
LOCUS       DBNGWZ010000590        12056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3490748, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000590 DBNGWZ010000000
VERSION     DBNGWZ010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3490748"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="AC16CX_18375"
     CDS             <1..120
                     /locus_tag="AC16CX_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR3014508.1"
                     /translation="VHSQVQLNAVARRLNERPRKTLNYETPAERFAQCVASVG"
     gene            252..1064
                     /locus_tag="AC16CX_18380"
     CDS             252..1064
                     /locus_tag="AC16CX_18380"
                     /inference="COORDINATES: protein motif:HMM:NF028004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3014509.1"
                     /translation="MKKVFGLSILLAVAPFAQAADIEAGKAKAATVCAACHGVNGVSV
                     SDAIPNLAAQRAGYLEAQLKFFKDGTRKEPGATSRAAIMNAIASQLSAEDIANVAAYF
                     SAQPGAATGAKSTFLPNVAKTSVTFPEGYKDTFTKYHTINFPATRQVRHYYANKTAVA
                     AAKAGKPLPDGSVLFVEVYAAKLDAGNKPVMGGDGFYVADKLLLYTAMAREAGWGKDI
                     PEMLRNANWNYAIFTTDKQHRTGVNQAECLACHKPLDSVSYAFTLKQLAEAK"
     gene            complement(1396..2322)
                     /locus_tag="AC16CX_18385"
     CDS             complement(1396..2322)
                     /locus_tag="AC16CX_18385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014510.1"
                     /translation="MKVGFIGLGTMGASMASNVRKAGFELVVNDLRREAAAPHLKAGA
                     VWADSARKVAEAADVVFTSLPGPREVEAVAEELLGGMRKGTAWFDLSTNSPTVVRRLS
                     ERFAAKAIAMLDSPVSGGPAGAKSGKLALWVSGDKAAFDKHKAVLDAIGDQAIHIGPV
                     GAGTIAKLVHNCAGYAILAALAEVFTLGVKAGVEPLALWAAVRQGAFGRRRSFDRLAE
                     QFLVGKFDPPAFALELAHKDVTLATDLGREFHVPLKIANLVHQEMTEALNREGWAKRD
                     SRIFMLLQEERAGVTIKVPPEKIKEVLESEKQ"
     gene            complement(2369..2821)
                     /locus_tag="AC16CX_18390"
     CDS             complement(2369..2821)
                     /locus_tag="AC16CX_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014511.1"
                     /translation="MCSGVEIEHGGKRTRVYFPNPKAALPARRQGGGCVMLPWGARGA
                     EYALDPVTREKTRWKWPQGGWARLESIEAGRWDTWSPRPARIPALAYMEKDADDARHW
                     FDLEPDQFIQGLVAELMGERRIYVVTISAPPQFARIHDRWPRIVGASR"
     gene            complement(2821..3252)
                     /locus_tag="AC16CX_18395"
     CDS             complement(2821..3252)
                     /locus_tag="AC16CX_18395"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3014512.1"
                     /translation="MFTQTRQFAATDSAFVAMRAFLDGVTAQAGWAKADTHRLTLILE
                     EIFTNTVKYGAAQAAGDAVTLTLTLGDDGRIAVDYRDCAPPHNPFERLSLDHLSEDTN
                     TRRIGGLGVPLILALAEDVRHAHADRGNRITFTLRPRAPNR"
     gene            3349..3828
                     /locus_tag="AC16CX_18400"
     CDS             3349..3828
                     /locus_tag="AC16CX_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HIR3014513.1"
                     /translation="MDVPERRCFRRIVAFMGVVALTVAFAAPAGASEAGQVKVVKGSV
                     VIERDGGRIPATVGAKVLRADTVRTGADGSVGIALLDNTLISAGPNSELIIARFAFNS
                     TTHSGELDASLRRGTLSMVSGKLAKQSPDAVRVTTPAAVLGARGTEFHVRVDETAPQ"
     gene            3981..4457
                     /locus_tag="AC16CX_18405"
     CDS             3981..4457
                     /locus_tag="AC16CX_18405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR3014514.1"
                     /translation="MTTPQGQTVLDRPYASAKVRPGAKPALATLSEREVKETFGAAIA
                     ARPKPPVSFTLYFLEGKDEYTPESKMVVEEVLRELASRQAPEIVVIGHTDRVGSLEFN
                     DRLSLQRAERVRGDFIQRGIAPESIRVAGRGERELLVSTVDEVPEPRNRRVEINIR"
     gene            complement(4454..6610)
                     /locus_tag="AC16CX_18410"
     CDS             complement(4454..6610)
                     /locus_tag="AC16CX_18410"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR3014515.1"
                     /translation="MAWWSRSSAARVPGDPPEPTRVHGAVRRGPVWALGAVVLAAVFA
                     AQLATTPVHRLLQLDLFDAYQALWPRSRQSAPVVIVAIDEASLERHGQWPWPRWLLGR
                     LVEAIAAAQPAAIGLDLLFPEPDRHSPARLAEYAPHITEGLAQELRRLPSNDALFAAA
                     LKDKPVVLAVAGVDEGVASPRVGRMAPFVIQGTDPLPKVRKYSGALRSVEMIDRAAAG
                     HGLISADLQRGVVRRVPLVSAIRETLAPALSLEMIRVASGTPALRVRSGRGGVESVGT
                     GGVMVPTDAEGHAWVRYSRHDPTRFLSAADVLDGRVAAAQLERRLVLVGLTGLGLVDY
                     PATPVDSRVPGVEVHAQLLENIFDGTHLIRPNWGPALEAGLLFSCGLLLVALAPVVRP
                     WLALLIAAAVAAAMFAAGGFAFLFTGMLIDTASPAAGGIAVFGVALGATLVESERQRR
                     RLRAELQTQREAAARLAGELEAARRVQMGMLPDPASVLANDARLDLAAFMEPAREVGG
                     DLYDFYRLTDDRLLFLVGDVSGKGLPASLMMAVSKSVCKGVALRGIASVDALLARANA
                     EIARDNPDFMFVTALACALDLNTGVLEYCSAGHEAACVLRAGGGVLVLEEGGGPPLCM
                     LDDYRYEVGRHALAPGETIVLVTDGITEAMDAGGALYGRARFRALLAGLAAEASAASV
                     IAAIRRDIATFCAGAEPMDDMTALALRWNGPARSRS"
     gene            complement(6588..7544)
                     /locus_tag="AC16CX_18415"
     CDS             complement(6588..7544)
                     /locus_tag="AC16CX_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3014516.1"
                     /translation="MRIRFWGTRGSLPAPLTAGDVTEKIATALDAANGRRFDTPEAMR
                     AFIDGLPFSVARTFGGNSSCVQIDEGGPCYILCDLGSGARLFGAEALKRHGEFTPQTY
                     HVFMSHLHWDHIMGFPFFPPAYIPGNRILIYGCHADLEQAFRRQHAAPSFPVDYSELK
                     ATIEFIRLEPGRTCEAAGLRVTPKLQRHGGDSYGFRFERDGKTVVYSTDSEHRPDEPA
                     ETEAFVRFFRDADLVIFDAMYSFADAVSVREDWGHSSNVVGVELCQMAGAKRLCLFHH
                     EPANDDAQLERLLKETTRLEEITRGDQPLQITSAYDGMVVEI"
     gene            complement(7555..7929)
                     /locus_tag="AC16CX_18420"
     CDS             complement(7555..7929)
                     /locus_tag="AC16CX_18420"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3014517.1"
                     /translation="MEVSVRRLGRVTVIAPAGRVDHLNAEAFRSLLAPHLASCAAGGE
                     CLILDLSRLEYISSAGLRILMIAAKQVGAQRGRIVVAAMQPVVAEIFEISRFTLVYEV
                     FATLPEALSALDAETPVLEQTV"
     gene            complement(7934..9100)
                     /gene="metC"
                     /locus_tag="AC16CX_18425"
     CDS             complement(7934..9100)
                     /gene="metC"
                     /locus_tag="AC16CX_18425"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HIR3014518.1"
                     /translation="MKKDTVLTHTGRDPARYEGSVNPPVYRTSTVLYPDLESFEAPRD
                     EDYQKFSYGRHGTPTTRALEEAVAKLEGGHRAVVLPSGLAAATTAISAFVKSGDHLLL
                     ADSVYTPTRNFCDRRLKPNGVEVEYYDPLAGAGIAKLLRPNTRAVYCESPGSLSFEVQ
                     DIPAIAGAAHARGVPVIADSTWGTPYFFRSFEHGVDVSVHSATKYIAGHADVVMGVIV
                     TNERHWRTVRRTVADYGYSVGSDDCYLALRGFRTIGVRLKHQMQSALTVATWLKSRPE
                     VRRVLYPALEGDPGHAIWKRDFSGAAALFSFVLDGASDGQAANFVNALKLFGIGSSWG
                     GFESLAVVARIEKYRTVTRWNPGGPTIRLHIGLEDPDDLVADLDAAFAQMRKAG"
     gene            9297..11591
                     /locus_tag="AC16CX_18430"
     CDS             9297..11591
                     /locus_tag="AC16CX_18430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3014519.1"
                     /translation="MADTHAPHHGNRVEDRRLITGAGKYASDWNLPGQLYACFVRSDR
                     AHAEIISVNVAKAAQHPGVKRVYTGEDALTAGYTKYFVIVSYPGRGGKHIIKPPRPVL
                     SARRVRHVGEAVAMVVADTPAAAQDAAELVEIEYRDLPAVVSAEEALAPGAPQLHEEA
                     PGNLAFDYEAGNEEAVTAALAQAAHVTRLKLAVSRVAPSPMEPRACTVRYDAVDGTYH
                     VHVCIQGINMMRRQLAAITDVPEDRLVVHAQDVGGSFGQRSAAYPEYAVQMIAAKELS
                     RPVKWISTRSEGFMSDTHGRANTVNGELALDRDGKFIALKLDWISDMGGYLSATGSAS
                     HTRNPSTCMTGVYRIPALYGRFRLAFTNTVPVAAYRGAGRPDIAYVIERLVNQAAAEL
                     KMDPAELRRRNFIPREAFPYKTPTGSTYEAADLHGVLDRALKLADWKGFPARRKQSEK
                     AGKLRGIGISTVIENTGAGLYPKDQVEMEVTTGGEVVAYSVSQSQGQGHETTLGMIVA
                     ETLGVPLERVRIRQGVKPLFGNHTGGSRTTVGAGSACKLAAQKLIEHARSAAAERLQI
                     EPSQVEYARGVFHARGTKQKIGLFELAQKRPINVMAEGSFGSTFPNGCHITEVEIDPA
                     TGVTRVDAYNAVDDCGNVINHAIVEGQVHGAVTQGAGQIFGEQVVYDRGTGQLLTGSF
                     MDYCMPRAGLVSAIRMEELPVPSLSNALGVKGVGESGCTASLPSLANAVMDALRPLGI
                     RHLDMPFTPAKLWHAIHSTKRGRP"
     gene            11684..>12056
                     /locus_tag="AC16CX_18435"
     CDS             11684..>12056
                     /locus_tag="AC16CX_18435"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3014520.1"
                     /translation="MSTKTHDHHDPIGRVEDARLTTGAGQYAADWNLPGQLYAYFVRS
                     DRAHAEIVSVSAAAALKHPGVKAVYTGADAVGAGYIKPMHTLTFPGKGGMKTRAPARP
                     ALAHRKVRFVGEALAMVVADTA"
BASE COUNT         2137 a         4032 c         3953 g         1934 t
ORIGIN      
        1 gttcactcgc aggtgcaact gaatgcggtg gccaggcggt taaatgaacg gccgaggaaa
       61 acgctaaact acgaaacacc agccgaacga tttgcccaat gtgttgcgtc ggtcggttga
      121 atccgcagcc aaaagcggac gctcacgcta aattgacagc gtcatggcat cagagtaact
      181 tgcgtcgcca aaaatcccca tgttggggtg taaaagctgg ttccacccat cttctacaga
      241 ggaaaagacc catgaaaaaa gtattcggat tgtccatcct tctggccgtt gcgccgtttg
      301 cgcaagccgc ggatatcgag gccggcaaag ccaaagccgc caccgtgtgc gcggcatgcc
      361 acggcgtaaa cggcgtgagt gtgagcgatg ccatccccaa tctcgcggcg cagcgggcgg
      421 gctaccttga ggcgcaactg aagtttttca aggacggcac gcgcaaggaa ccgggtgcga
      481 cgagccgggc ggcgatcatg aatgcgattg cgtcccagct cagcgccgag gacatcgcca
      541 acgtcgcggc gtacttttcc gcgcagccgg gtgctgcgac cggcgccaag tccacgtttc
      601 tgccgaacgt ggcgaagacc agcgtgacct ttcccgaagg ctacaaggac acgttcacga
      661 agtaccacac gatcaacttc cccgcgacca ggcaggtgcg ccactactat gccaacaaga
      721 ccgcggtggc ggcggcgaag gcgggcaagc cgctgcccga cggctcggtg ctgttcgtcg
      781 aagtgtatgc ggcgaaactc gacgcaggaa acaagccggt gatgggcggc gatggcttct
      841 acgtggcgga caaactcctc ctctacacgg cgatggcgcg cgaagcaggc tggggcaagg
      901 acatccccga aatgcttcgc aacgcgaact ggaactacgc gatcttcact accgacaagc
      961 agcaccggac cggagtgaac caggccgaat gcctcgcctg ccataaaccg ctcgacagcg
     1021 tcagctacgc cttcacgttg aagcagctcg cggaagcgaa gtaagccggt gccttagcaa
     1081 ctgcccaatc cgggcagccg gggccgtatc gatgacgcga acaatcggaa acgatccggg
     1141 acagacgacc atttccggca ggtgacagga aacaagccgc ctaacacgca tccgcactga
     1201 tacagacccc gcttcggcgg ggttttgttt gtgtcgtgaa cgtccgctgt gggtggcgtt
     1261 tccagacatt ggcgctcgga cgctgcctgt cacattaagt cggagctact acacttcaac
     1321 tctccgtgtt ctctgtgatc tctgtggctt gatcttgatt ttcatcggcg ttaatcggcg
     1381 tttatctgcg gctaattatt gtttctccga ttcgagcact tccttgatct tctccggcgg
     1441 caccttgatc gtgacgccgg cgcgctcttc ctgcagcagc atgaagatgc gcgagtcgcg
     1501 cttggcccag ccctcgcggt tcaacgcctc cgtcatctcc tggtgcacca ggttcgcgat
     1561 cttcagcggg acgtgaaatt cgcggccgag gtcggtggcg agcgtcacgt ccttgtgggc
     1621 gagctcgagc gcgaacgcgg gcgggtcaaa cttcccgacc aggaactgct cggcgaggcg
     1681 gtcgaacgac cgccgccggc cgaaagcgcc ctggcgcacc gccgcccaca gcgcgagcgg
     1741 ctcgacgcca gcttttaccc ccaatgtgaa cacttcagcg agcgcggcca gtatcgcgta
     1801 gcccgcgcag ttgtgcacga gcttggcgat ggtgccggcg ccgaccggcc cgatgtggat
     1861 cgcctggtcg ccgatggcgt cgagcacggc tttgtgcttg tcgaaagccg ccttgtcgcc
     1921 gctcacccac agcgcgagct tgccgctctt cgcgcccgcc ggcccgccgc tcaccggtga
     1981 gtccagcatc gcgatggcct tcgccgcgaa gcgctcgctc aggcggcgca ccacggtggg
     2041 cgagttggtg gaaaggtcga accatgccgt gcccttgcgc atgcccccga gcagctcttc
     2101 cgccacggcc tcgacctcgc gcgggcccgg cagcgacgtg aacacgacgt ccgccgcctc
     2161 ggccaccttg cgcgccgagt cggcccagac cgcaccggcc ttgaggtgcg gcgcggcggc
     2221 ctcgcgccgc aggtcgttga cgacgagctc gaagcccgcc ttgcggacgt tcgacgccat
     2281 gctggcgccc atggtgccga ggccgatgaa tcccaccttc atgttgtttt ctcctgtaat
     2341 gactctctaa aaagtcttcc ctcgatcgtc agcgcgaagc gccgacgatc ctcggccagc
     2401 gatcgtggat gcgggcgaac tgcggaggcg cggagatcgt caccacgtag atgcggcgct
     2461 cgcccatcag ctcggcgacc agtccctgga tgaactgatc cggttccagg tcgaaccagt
     2521 gccgggcgtc atcggcgtcc ttttccatgt aggcgagcgc gggtatccgc gcgggacgcg
     2581 gcgaccaggt gtcccaccgg cccgcctcga tggattccag ccgtgcccag ccgccctgcg
     2641 gccacttcca ccgggttttt tcccgcgtga cgggatcgag cgcgtattcc gcgccgcgcg
     2701 cgccccacgg cagcatgacg cagccgccac cctggcgccg ggcgggcagg gcggctttgg
     2761 ggttggggaa ataaaccctg gttcgcttgc ccccgtgctc gatctcgacg ccgctgcaca
     2821 ttagcggttt ggcgcgcggg gtcgcaacgt gaaagtgatg cggttgccgc ggtcggcgtg
     2881 agcgtgacgc acgtcctccg ccagggcaag aataagaggg accccgaggc ctccgatacg
     2941 gcgggtgtta gtgtcctcgc tcagatgatc cagggaaagc cgctcgaacg gattgtgcgg
     3001 cggcgcgcag tcccggtaat ccaccgcaat ccgcccgtcg tcgccgagcg tcagggtcag
     3061 ggtcacggca tccccggcgg cctgcgccgc gccatatttc acggtattgg tgaagatttc
     3121 ttccaaaatc aaagtcaggc ggtgcgtgtc cgcttttgcc catccggcct gggcggttac
     3181 gccgtccaga aaagcccgca tcgcgacgaa agcgctgtcg gttgcggcaa attgccgggt
     3241 ttgcgtaaac atgccgcgtg ggcgggggaa ttttctggca ttatagacgc tcccatggcc
     3301 ggctcttttg ccggtcgcgg gtgcggtgct cgtgaaaggg ggacggccat ggatgtaccc
     3361 gagagaagat gtttccgcag gatagtcgcc ttcatgggag tagtcgcgtt gacggttgct
     3421 ttcgctgccc cggcaggcgc gagcgaagcg ggccaggtga aggtcgtcaa aggctcggtc
     3481 gtcatcgagc gcgacggcgg acggattccc gcgacagtcg gcgccaaagt gctgcgggca
     3541 gacacagtgc gcaccggcgc tgacgggtcc gtgggcatcg cgctgctcga caacacgctg
     3601 atctcggcgg ggcccaacag cgaactgatc atcgcgcgct tcgcattcaa ctccacgact
     3661 cacagcggcg agcttgacgc ttctctccgg cgcggcaccc tctccatggt ttcgggaaaa
     3721 ctcgccaagc agtctcccga tgcggtgcgc gttacgacgc cggccgcggt gctcggcgcc
     3781 cgcggcaccg agttccacgt gcgggttgac gagaccgcac cgcagtagaa ccgcctcggt
     3841 ccatgcgcca gttcgcgtcc accgggtggt gcaccctgtg cctgctcgct gcgggttgcg
     3901 ccacacctcc acccgcaccc aagccacaga caaccgcggt cgtcgtgcca gatgcggacg
     3961 gcaaagtcgg cgctgtcgtc atgacaacgc cgcagggcca gaccgtgctc gaccgtccgt
     4021 atgcgtcggc caaagtacgt cccggcgcca aaccggcgct ggccacgctc tcggagcgcg
     4081 aggtgaaaga gacgttcggg gcggccattg ccgcccgccc caagccgccg gtttcgttca
     4141 cgctttactt ccttgagggc aaggacgaat acacgccgga atcgaagatg gtggtcgagg
     4201 aggtgctgcg cgagctggcg agccgccagg cacccgagat cgtggtcatc gggcacaccg
     4261 acagggttgg ctcgctcgag ttcaacgacc gcctctccct gcagcgtgcc gaacgggtgc
     4321 gcggcgattt catccagcgc ggaatcgcgc cggaatcgat ccgggtagcc gggcgtggcg
     4381 agcgcgaact cctcgtttcc acggtggacg aggtgccgga accgcgcaat cggcgggtgg
     4441 agatcaacat ccgctagctc cggctgcgag cggggccgtt ccagcgcagg gcgagcgccg
     4501 tcatgtcgtc catgggttcc gctccggcgc agaatgtcgc gatatcgcgg cggatcgccg
     4561 cgatgaccga tgccgcgctc gcttctgcgg cgagccccgc cagcaaagcc ctgaacctcg
     4621 cgcgcccgta aagtgcgccc ccggcatcca tcgcctcggt gattccatcg gtgaccagaa
     4681 cgatggtctc gccgggcgcc agtgcgtggc gccccacctc gtagcggtag tcgtccagca
     4741 tgcataacgg cggaccaccg ccctcctcga gcacgagcac gccaccgccg gcgcgcagga
     4801 cgcacgccgc ttcgtgcccc gcgctgcagt attcaagcac gcccgtattc agatccagag
     4861 cgcaggcaag ggcggttacg aacatgaaat ccggattgtc gcgcgcgatt tcggcgttcg
     4921 cccgggcgag caacgcgtcc acggacgcga ttccgcgcag cgccacgcct ttgcacaccg
     4981 acttgctcac cgccatcatc aggcttgccg gaagcccctt gcccgacacg tcgcccacca
     5041 agaacagcag gcggtcgtcg gtcagccggt aaaaatcgta caggtcgccg cctacttccc
     5101 gcgccggctc catgaaagcg gcaagatcga ggcgcgcgtc attcgccaac acggacgccg
     5161 gatcgggcaa catgcccatc tggacgcggc gcgccgcttc caactcaccc gcgaggcgag
     5221 cagcagcttc gcgctgggtc tgtaactcgg cgcgcagccg ccggcgctgg cgttccgatt
     5281 ccacgagtgt tgcgccgagc gcgacgccga ataccgcgat gccgcctgcg gcaggcgagg
     5341 ccgtgtctat cagcatcccc gtgaacaaaa acgcgaaccc gccggcggcg aacatcgccg
     5401 cggccaccgc cgcggcgatg agcaacgcga gccatggccg taccacggga gccaacgcga
     5461 cgagcagcag cccgcatgag aacagcaacc ccgcctcgag cgccggaccc cagttcgggc
     5521 ggatcaggtg cgtgccgtcg aaaatgttct ccagcagctg cgcgtgcacc tcgacgccgg
     5581 gcacgcggct gtccaccggc gtcgcaggat aatcaacgag ccccaacccg gtcaggccga
     5641 ccagcaccag cctgcgttcg agctgggccg cagcgacgcg gccgtccaag acatcggcgg
     5701 ccgataaaaa acgcgtggga tcatggcggc tgtaacgcac ccacgcatgg ccttcggcat
     5761 cagtcgggac cattacgcca ccggttccga ccgattcgac gccgccgcgg ccgctgcgca
     5821 cccgcagcgc tggagtgccg ctcgcaaccc gtatcatctc gaggctcaac gcgggagcaa
     5881 gggtttccct gatggcgctt accagaggca cgcgccgtac gacccctcgt tgcaagtcgg
     5941 cgctgatcaa gccatggccc gcggcggcac ggtcaatcat ctcgacgctg cgcagcgcgc
     6001 cgctgtactt gcgcactttc ggcaggggat ccgtgccttg aataacgaac ggagccatcc
     6061 ggccgacgcg aggcgaggcg accccttcat ccacgccggc gaccgccaat accaccggct
     6121 tgtccttgag cgccgcggcg aacaaggcgt cgttgctggg cagcctgcgc aactcctgcg
     6181 cgaggccctc ggtgatgtgg ggcgcgtatt cggccaagcg cgccggcgaa tggcggtcag
     6241 gctcggggaa cagcagatca agaccgatag cagccggctg cgccgcggcg attgcctcca
     6301 cgaggcggcc aagcaaccac ctcggccacg gccattgccc gtggcgctca aggcttgctt
     6361 cgtcgattgc cacgatcacg acgggcgccg attggcgcga ccgcggccac agggcctgat
     6421 aggcatcgaa caagtcgagt tgcagcaggc ggtgcaccgg ggttgttgcg agttgcgccg
     6481 cgaacactgc cgccagtacc accgccccca gcgcccagac cggaccccgg cgcacggcgc
     6541 cgtggacgcg cgtgggttcc ggcgggtcgc ccggcaccct tgcggcgcta gatctcgacc
     6601 accatgccat cgtaggcgct cgtgatttgc agcggttgat cgccccgggt gatctcttcg
     6661 aggcgggtgg tttccttcaa gaggcgttcg agttgcgcgt cgtcgttggc gggctcgtgg
     6721 tggaatagac agagacgctt cgctccggcc atctggcaca gttccacgcc gaccacgttg
     6781 ctggagtggc cccagtcctc cctgacggaa accgcatcgg cgaacgagta catggcatcg
     6841 aagatcacga gatccgcgtc gcggaaaaac cgtacgaagg cctccgtctc ggctggttca
     6901 tccggacggt gctcggaatc cgtggaatag acgaccgtct tgccgtcccg ctcgaaacga
     6961 aatccgtacg agtcgccgcc gtggcgctgg agcttcggcg ttacgcgcag ccccgccgcc
     7021 tcgcaggtcc gccccggttc cagccggatg aactcgatcg tcgccttcag ctccgaataa
     7081 tccacgggaa aggacggcgc ggcgtgctgg cggcgaaaag cctgctcgag gtccgcatgg
     7141 caaccgtaga tgaggatgcg atttcccggg atgtacgccg gcgggaaaaa ggggaatccc
     7201 atgatgtggt cccagtgcag gtgagacatg aacacatggt aggtctgggg ggtgaattcc
     7261 ccgtggcgct tcagcgcctc ggccccgaac agccgcgccc ccgaacccag gtcgcaaaga
     7321 atgtagcagg ggccgccttc gtcaatctgg acgcaggagg agttccctcc gaaagtccgc
     7381 gccacggaaa acggcagccc gtcgatgaag gcgcgcatgg cttcgggcgt atcgaacctt
     7441 cgcccgttgg cggcgtccag cgccgtcgcg attttttccg tgacgtcgcc cgccgtcagc
     7501 ggcgcgggca gcgaaccgcg agtgccccag aagcggatgc gcatcgaatc agcctcagac
     7561 ggtttgctcc agcacgggcg tttccgcgtc gagcgcggag agcgcctcgg gcagcgtggc
     7621 gaaaacttcg tataccaggg tgaagcgact gatttcgaat atttcggcca caacgggttg
     7681 catcgcggcg accacgatgc gccccctttg cgcaccgacc tgttttgcgg cgatcatcag
     7741 gatgcgcagc ccggcgctgc tgatgtattc gagccgggac agatccagaa tcaggcattc
     7801 gcctccggcc gcgcacgagg caagatgcgg cgcgagcaac gaacggaacg cctcggcgtt
     7861 cagatgatca acgcggccgg ccggcgcgat caccgttacc ctccccagcc gccggaccga
     7921 aacctccatg aggctaacct gcttttctca tctgcgcgaa ggcggcatcg agatcggcga
     7981 cgagatcgtc cgggtcctcg agcccgatgt ggagccgaat cgtcgggccg cccgggttcc
     8041 atctcgtgac ggtgcggtat ttctcgatcc gggcgacgac ggcgaggctt tcgaagccgc
     8101 cccagctcga gccgatgccg aagagcttga gcgcgttcac gaaattcgcg gcctggccgt
     8161 cgctcgcgcc gtcgaggacg aaggagaaca gcgccgccgc gccgctgaaa tcgcgcttcc
     8221 agatggcatg gccggggtcg ccttcgagcg ccgggtagag cacgcgcctg acttccggcc
     8281 gcgacttgag ccaggtggcg accgtcagcg cgctttgcat ctggtgtttg agccgcacgc
     8341 cgatcgtgcg gaagccgcgc agcgcgagat agcagtcatc cgaaccgacg ctgtagccgt
     8401 agtcggcgac cgtcctgcgc accgttctcc agtgccgctc gttcgtgacg atgacgccca
     8461 tcaccacgtc ggcgtggccg gcgatgtact tggtcgccga atggacggag acgtccaccc
     8521 cgtgctcgaa cgagcggaag aaatacggcg tgccccaggt gctatcggcg atgacgggaa
     8581 cgccgcgcgc atgcgcggcc ccggcgatcg cggggatgtc ctgcacttcg aagctgagcg
     8641 agcccggcga ttcgcagtag acggcgcggg tgttggggcg caggagcttc gcaatgcccg
     8701 cgccggccag cggatcgtaa tactcgacct cgacaccgtt gggtttcagc cgccggtcgc
     8761 agaagttgcg cgtcggtgta tagaccgagt cggcgaggag gaggtggtcg ccgctcttca
     8821 cgaacgcgct gatcgcggta gtggctgccg ccagccccga cggcagcacg accgcgcgat
     8881 ggccgccttc gagcttcgcc accgcctctt ccagcgcccg cgtggtgggg gttccgtggc
     8941 ggccgtagct gaacttctgg taatcctcgt cgcggggcgc ctcgaagctt tcgaggtcgg
     9001 ggtaaagcac ggtcgaggtc cggtacacgg gcggattgac cgaaccctcg tagcgggccg
     9061 ggtcgcgccc cgtgtgtgtc aatacggtgt ctttcttcat gatgggggat cagaccgggg
     9121 atattctgga ttctcgggca attgtgcgcg gcgcaccagc ccggtgcaac atatggactc
     9181 gcggattgct ggcaggcgcg gtatgcccga taatctgcgc ccagcgccct gcgcgcaagg
     9241 cgtcaagagg cgcacccccg caggttccag caatcggact ttcccggaag gcttgcatgg
     9301 ccgacacgca cgcgccgcat cacggcaaca gggtggaaga ccgccggctc atcaccggcg
     9361 ccggaaagta cgcctccgac tggaacctgc ccggccagct ctacgcctgc ttcgtgcgct
     9421 ccgaccgcgc gcacgccgag atcatctcgg tgaacgtcgc aaaagcagcg cagcacccgg
     9481 gcgtgaagcg cgtctatacc ggcgaggacg cgctcaccgc cggctacacc aagtacttcg
     9541 tgatcgtgag ctacccgggc cgcggcggga aacacatcat caagccgccg cgcccggtgc
     9601 tgtcggcgcg cagggtgcgg cacgtgggcg aggcggtggc gatggtggtc gcggacacgc
     9661 ccgcggcggc gcaggacgcg gcggaactcg tcgagatcga gtaccgtgac ctgccggcag
     9721 tggtttcggc cgaggaagcg ctcgcccccg gcgcgccgca actgcacgag gaggcgccgg
     9781 gcaatctcgc cttcgactac gaggcgggca acgaggaagc cgtgacggcg gcgctcgcgc
     9841 aggccgcgca cgtgacgcgg ctcaagctcg cggtgtcacg ggtcgcgccc agcccgatgg
     9901 agccgcgcgc gtgcacggtg cgctacgacg cggtcgacgg cacctaccac gtgcacgtat
     9961 gcatccaggg catcaacatg atgcgccggc agctcgccgc catcaccgat gtcccggaag
    10021 acaggctcgt ggtgcacgcg caggacgtgg gcggcagctt cgggcagcgg agcgccgcct
    10081 acccggagta cgccgtgcag atgatcgcgg cgaaggagct cagccggccg gtcaagtgga
    10141 tctcgacgcg ctcggaaggc ttcatgagcg acacccacgg gcgcgccaac acggtgaacg
    10201 gcgagctcgc gctcgaccgc gacgggaaat tcatcgccct gaagctcgac tggatctccg
    10261 acatgggcgg ctatctctcg gcgaccggct cggcgagcca cacgcgcaac ccttccacct
    10321 gcatgaccgg cgtctaccgg atcccggcgc tctacgggcg gttccggctc gctttcacca
    10381 acaccgttcc cgtcgccgcc taccgcggcg ccggccgtcc tgatatcgcc tacgtgatcg
    10441 agcgcctggt gaaccaggct gcggccgagc tcaagatgga cccggcagag ctgcgcaggc
    10501 gcaatttcat tccgcgcgag gcattcccct acaagacccc gaccggctcg acctacgaag
    10561 ccgccgacct gcacggcgtc ctcgaccgcg cgctgaagct tgccgactgg aaaggcttcc
    10621 ctgcgcgccg caaacaatcc gagaaggccg ggaaactccg cggcatcggc atctcgacgg
    10681 tgatcgagaa taccggcgcg ggcctctacc ccaaggacca ggtcgagatg gaggtgacga
    10741 ccgggggcga ggtggtcgcc tattctgtgt cgcagtcgca gggacagggc cacgagacga
    10801 cgctcggcat gatcgtcgcg gaaacgctgg gcgtgccgct cgagcgcgtg cgcatccggc
    10861 agggcgtgaa gccgcttttc ggcaaccaca ccggcggctc gcgcaccacc gtgggcgcag
    10921 gcagtgcatg caagctcgcg gcgcagaagc tgatcgagca cgccaggtcg gctgcggcgg
    10981 aacgcctgca gatcgagccc tcacaggtgg aatacgccag gggcgtgttc cacgcgcgcg
    11041 ggacgaaaca gaaaatcggt ctgttcgagc tcgcgcagaa gcggccgatc aacgtgatgg
    11101 ccgagggcag cttcggctcg actttcccca acggctgcca catcacggag gtggagatcg
    11161 atcccgcgac cggcgtgacg cgcgtcgacg cctacaacgc cgtcgacgat tgcggcaacg
    11221 tcatcaacca cgccatcgtc gagggccagg ttcacggcgc ggtgacgcag ggcgccgggc
    11281 agatcttcgg cgagcaggtg gtctacgacc gcggcaccgg gcagcttctc accggcagct
    11341 tcatggatta ctgcatgccg cgcgcgggcc tggtgtccgc gatccgcatg gaggagcttc
    11401 ccgtgccgag cctgagcaat gcgctgggcg tgaagggcgt gggcgagtcg ggctgcaccg
    11461 cctcgctgcc ctcgctcgcc aacgcggtga tggacgcgtt gcggccgctc ggcatccggc
    11521 acctcgacat gccgttcacg ccggcgaagc tgtggcatgc gattcacagt acgaagagag
    11581 gcaggccgta agccgtaagc ggtcagcggt gagccgatga tcagctattt tctttgggtt
    11641 tgacggctta ccgcttactg ttcaccgctt accgttaatt gatatgagca cgaagacaca
    11701 cgatcatcat gatcccatcg ggcgcgtcga ggacgcgcgg ctgacgaccg gcgccggcca
    11761 gtacgcggcg gactggaacc tgcccgggca gctctacgcg tatttcgtgc gctcggaccg
    11821 cgcgcacgcc gagatcgtct cggtcagcgc cgcagccgcg ctcaagcacc cgggcgtgaa
    11881 ggcggtctac accggcgccg acgcggttgg cgcgggctac atcaagccga tgcacacgct
    11941 gaccttcccg gggaagggcg gcatgaagac ccgcgctccc gcacggcccg cgctcgccca
    12001 ccgcaaggtg cgcttcgtcg gcgaggcact ggcgatggtg gtggcggaca ccgcag
//
LOCUS       DBNGWZ010000591         2380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2483215, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000591 DBNGWZ010000000
VERSION     DBNGWZ010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2483215"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC16CX_18440"
     CDS             complement(<1..507)
                     /locus_tag="AC16CX_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3014521.1"
                     /translation="MLQEIVISNSFENLGLSAELLRAVAEQGYTEPTPIQAQAIPIVL
                     SGRDLLGAAQTGTGKTAGFTLPLLQRLATGAHTSASPARHPVRALILVPTRELAAQVQ
                     ESVRTYGRHLKLRSAVIFGGVGFNPQATELRRGVDIVVATPGRRLAHVEQRTIDLRPV
                     ELRVLDEAD"
     gene            complement(680..>2380)
                     /gene="mnmC"
                     /locus_tag="AC16CX_18445"
     CDS             complement(680..>2380)
                     /gene="mnmC"
                     /locus_tag="AC16CX_18445"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF002480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmC2/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC1"
                     /protein_id="HIR3014522.1"
                     /translation="PARSDRLHFVSVEKHPFTREGLATLHARYPELAPLAAELQAAWP
                     LLLPGLHRLPFEDGRVTLTLALADVADVLPKLRLAADAFYLDGFAPERNPAMWTPAVA
                     KALARLARPGATVATYTTARAVRDALAAAGFATELRPGFGRKRQMLAARFAPARPLRH
                     GAPAAPAWSERRAIVIGAGLAGAGVAERLVARGWKVELVERHAAPAAEASGMAAGSFH
                     PHVSRDDSLLSRTTRAGFLHALRCWRALEGSGHRFAWAQCGVLQIARDAREERRMADS
                     VRALGIPTGFVDYLPRADAGRVTGLAAQTGGLWFPEGGWVRSSQLVLALLDAARPGLA
                     FHHSRTVHALVREADRWHALAADGALIAAAPVAVLTNSHDATRLVPLGVELKRVRGQL
                     TCLPPGSLDAPGMVLAGAGHLIPLPDGAAVVGSTYDFEDEDPEPRASGHAGNLERLEQ
                     LLPGSAARLDPARLAGTVGFRCVTPDRLPLIGALADFDAANGRPAAIGGTRVRDLPRL
                     PGLYGAFGYASRGLTWAALGGELIAAAVEGEPLPLEGDLADAVDPGRFLLRRARRGRL
                     "
BASE COUNT          371 a          833 c          864 g          312 t
ORIGIN      
        1 gtcggcctcg tccagcaccc ggagttcaac ggggcgcagg tcgatcgtgc gctgctcgac
       61 gtgggcgagc cgccgccccg gcgtcgcgac gacgatgtcc acgccgcggc gcagttccgt
      121 ggcctgcgga ttgaagccga cgccgccgaa gatcacggcc gagcgcaact tgagatgcct
      181 gccgtaagtg cggacgcttt cctgcacctg cgccgcgagc tcgcgggtcg gcaccaggat
      241 cagtgccctg acgggatggc gcgcaggcga agcgctggta tgggcgccgg tcgcgagccg
      301 ttgcagcagc ggcagcgtga agccggcggt cttgccggtg ccggtctgcg ccgcgccgag
      361 cagatcgcgc cccgagagca cgatcgggat ggcttgcgcc tggatggggg tgggttcggt
      421 gtaaccctgc tcggcgacag cgcggagcag ttcggccgac aggccgagat tttcgaatga
      481 attgctaatg acaatctcct gaagtatcgg cccgggaaga cgcgagtctt ccaaaccggt
      541 tcaggcagat gcgatttagg ctgggtgatt cgacggtccc ggatggaccg tgaaacgact
      601 gagacgctaa ccggacgatg cgaaacagaa accttggcgc gcactataca cgagtttgcc
      661 ccgcgcgtcc ggactatctt tacagcctcc cgcgccgcgc gcgcctcagc aggaagcggc
      721 cgggatccac cgcgtcggcg aggtcgcctt cgagcggcag cggttcaccc tcgaccgcgg
      781 ctgcgatcag ctcgccgccg agcgcggccc aggtgagccc gcgcgaggca tagccgaacg
      841 caccgtagag cccgggcaac cgtggcaagt cgcgcacgcg cgttccaccg atagcggcgg
      901 gccggccgtt tgcggcgtcg aagtccgcca gcgcgccgat cagcggcagg cggtccggcg
      961 tcacgcagcg aaatcccacg gtgccggcaa gccgggcggg gtcgagccgc gcggcgcttc
     1021 ccggcaacaa ttgttcgagc cgctcgaggt tgccggcatg tccggatgcg cgcggctcgg
     1081 ggtcttcgtc ctcgaagtcg taggtcgagc ccacgaccgc cgcgccgtca ggcagcggga
     1141 tcaggtgacc ggcgccggcc agaaccatgc cgggagcatc gaggctgccg ggcggcaggc
     1201 aggtgagctg cccgcgcacg cgcttcagtt ccacgccgag cgggacgagg cgcgttgcgt
     1261 cgtgcgagtt ggtgagcacg gccaccgggg ccgccgcgat cagcgcgccg tccgccgcga
     1321 gcgcgtgcca gcgatccgcc tcgcgaacca gcgcgtgcac cgtgcggctg tggtggaagg
     1381 ccagacccgg gcgggccgcg tcaagcagcg ccaacacgag ctgcgatgac cggacccacc
     1441 cgccctccgg aaaccacagg ccgccggttt gcgccgcgag cccggtcacg cgcccggcgt
     1501 cggcgcgcgg caggtaatcg acaaagccgg tcggtattcc cagcgcgcga accgaatccg
     1561 ccatccgccg ctcctcgcgc gcgtctcttg cgatctgcag cacgccgcac tgtgcccatg
     1621 cgaagcggtg gccgcttccc tcgagcgcgc gccagcagcg gagcgcatgc aaaaatcccg
     1681 cacgtgtggt ccgcgagagc aggctgtcat cgcgcgagac gtgcggatga aagctgccgg
     1741 cggccatccc cgatgcctcc gccgccgggg cggcgtggcg ctcaaccagt tcgaccttcc
     1801 agccgcgcgc gacgagccgc tccgcgacgc cggcgcccgc caggcccgcg ccgatgacga
     1861 tggcgcggcg ctcgctccag gccggcgcgg cgggcgcacc gtgtcgtaaa ggtctggctg
     1921 gggcaaagcg tgccgcgagc atctggcgct tacggccgaa gccggggcgc aactcggttg
     1981 cgaaaccggc ggcggcgagc gcgtcgcgca cggcgcgcgc cgtcgtgtag gtagcgaccg
     2041 tcgcgccggg ccgcgcaagc cgcgccagcg ctttcgcgac cgcgggcgtc cacattgccg
     2101 gattgcgctc cggcgcgaag ccgtcgaggt agaacgcgtc agcggcgagc ctgagctttg
     2161 gcagcacgtc ggcgacatcg gcgagcgcaa gcgtgagcgt cacgcgcccg tcctcgaaag
     2221 gcagccggtg caggccgggc agcagcagcg gccaggcggc ctgcaactcc gctgcgagcg
     2281 gcgcgagttc gggatagcgc gcgtgcagcg tcgccagtcc ctcgcgcgtg aacggatgct
     2341 tctcgaccga cacgaaatgc aggcgatccg accgcgccgg
//
LOCUS       DBNGWZ010000592         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_465727, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000592 DBNGWZ010000000
VERSION     DBNGWZ010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_465727"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..998)
                     /locus_tag="AC16CX_18450"
     CDS             complement(33..998)
                     /locus_tag="AC16CX_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014523.1"
                     /translation="MKALIPVAVMTTLTMVVAGAMAQPYPAKSIRVIAGFPPGSGADI
                     TARVIGARLNDALGQQVVVDNRPGAASNIAADLAAKSPPDGYTLFIGTVANTINATLY
                     SKLPFDFVRDFAPVALTTATPNVLVVHPSVPARSVKELVALAKGRPGQLNFASAGTGT
                     APHMSGELFKAMAGVDMLHIPYKGSPPAVTDLLAGEVALMFSPSSTVLPHVKTGRLRA
                     LAVTTLTRLPSLPDLPTVAESGLKGYETITWFGFVAPAKTPHAIVTRLNAEIVKVLSA
                     PEVRRQFAAQGIETLGGTPEQFAAYIRDEIAKWAKVIKFSGARAD"
     gene            complement(1028..2248)
                     /locus_tag="AC16CX_18455"
     CDS             complement(1028..2248)
                     /locus_tag="AC16CX_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3014524.1"
                     /translation="MSRAKGVREVGYVDVRQGGGVRHTHAAAGRRMRPASWFDCAGGG
                     QVVVEKGIAYIGTMRNPHGTLIVDVSDPKRPKQLASLSMPTGTHSHKVRVASGIMVTN
                     REVLPRHTLQGEIPPTEFRGGLGIYDVSNPARPKLISSWEVTGGSGVHRFDFDGRYAY
                     ISPTADGYVGNIVMILDLKNPAQPEEVGRWWMPGQWVAGGEQPTWKGDAHKCHHPLRH
                     GNRLYTSYWHGGLVILDIEDMSKPRFVSGLDWSPPFPWPTHTCLRIPFKIQNRDFMLV
                     TDEDVFRVEEHPVHPAAFLWMVDITDERHPQPISTFQVEDMPDAPQPRMTGCHQPCEK
                     VTGTEIPVAWFAYGLRIIDISRPHALKEVAYYLPDVPQGSERVQSNDVTVDERGLIYL
                     LDRVRGLHILERIG"
BASE COUNT          410 a          783 c          803 g          437 t
ORIGIN      
        1 ctcccgctct cacgttcatc tgccgcatcc cctcagtccg cccgtgcacc ggagaacttg
       61 atgaccttgg cccatttggc gatctcgtcg cggatgtagg ccgcgaactg ctcgggcgtg
      121 ccgccgaggg tctcgatgcc ctgtgctgcg aactggcgtc gcacttcggg agccgacagc
      181 accttcacga tctcggcgtt gagccgcgtc acgatcgcgt ggggtgtttt cgccggggcc
      241 acgaacccga accaggtgat cgtttcgtag cccttgaggc cggactcggc gaccgtcggc
      301 aggtcgggca gcgacgggag gcgcgtcagg gtggtgaccg cgagcgcgcg cagccgcccc
      361 gtcttcacgt gcggcagcac cgtggaggac ggcgagaaca tcagcgcgac ctcgcccgcg
      421 agaagatcgg tgaccgccgg cgggctgccc ttgtacggga tgtgaagcat atcgacgccg
      481 gccatcgcct tgaacagctc gcccgacatg tgcggcgcgg tgccggtgcc ggccgaggcg
      541 aaattgagct ggccggggcg acccttcgcg agggcgacca gctccttgac cgatcgcgcc
      601 ggcaccgagg ggtgcactac cagcacattg ggcgtggcgg tcgtgagcgc caccggcgcg
      661 aagtcgcgca cgaagtcgaa aggaagcttg gaatagagcg tcgcgttgat ggtgttcgcg
      721 accgtgccga tgaacagcgt atagccgtcg ggaggcgatt tggccgcgag gtcggccgcg
      781 atgttgctgg ccgctcccgg gcgattgtcc acgacgacct gctggccgag cgcatcgttc
      841 agcctcgcgc cgatgacgcg cgcggtgatg tcggcgcccg agcccggcgg gaagcccgcg
      901 atcacccgga tcgacttggc cggatagggc tgcgccatcg ccccggctac aaccatcgtc
      961 aaggtcgtca tcacggcaac agggatcagc gctttcatgc gagcactcgg agccggcgcg
     1021 attacagtca cccgattcgt tcgagaatat gaaggccgcg cacgcggtcg agcaggtaga
     1081 tcagcccgcg ttcgtccacc gtgacgtcgt tgctttgcac ccgctccgat ccctgcggca
     1141 cgtccggcag atagtaagcc acttccttga gcgcgtgggg gcgtgaaatg tcgatgatgc
     1201 gcaaaccata ggcgaaccac gcgaccggga tctccgtgcc ggtgactttc tcgcacggct
     1261 ggtgacagcc ggtcatgcgc ggctgcggcg cgtcgggcat gtcctcgacc tggaaggtgg
     1321 agatcggttg cgggtgcctc tcgtcggtga tgtccaccat ccacaggaat gccgcaggat
     1381 gcacgggatg ctcttccacc cggaacacgt cctcgtcggt taccagcatg aaatcgcggt
     1441 tttgaatctt gaacgggatg cgcaggcagg tatgggtcgg ccacgggaac ggcgggctcc
     1501 aatcgagccc cgagacgaac cggggcttgc tcatgtcctc gatgtcgaga atcacgagcc
     1561 cgccgtgcca gtaactcgtg tagagccggt tgccgtgacg cagcgggtga tggcatttgt
     1621 gagcgtcgcc cttccaggtc ggctgctcgc cgccggcgac ccactgcccc ggcatccacc
     1681 agcggccgac ttcctccggc tgcgccgggt tcttcaggtc gaggatcatc acgatgttgc
     1741 cgacgtagcc gtccgcggtc ggcgagatgt aggcgtagcg cccgtcgaaa tcgaagcggt
     1801 gaacgccgga ccctccggtc acctcccagc tcgagatgag cttcggcctg gcagggttgg
     1861 aaacatcgta gatgccgagc cctcctcgaa actccgtcgg cgggatttcg ccctgaagcg
     1921 tgtgcctggg cagcacctcg cggttggtca ccatgatgcc gctcgccacc cgcaccttgt
     1981 gcgaatgggt accggttggc atggataacg aagcgagctg cttcgggcgc ttcggatccg
     2041 aaacgtccac gatcaacgtg ccgtgcggat tgcgcatggt gccgatgtag gcgatgccct
     2101 tctccacgac cacctgcccg ccgcccgcgc aatcaaacca cgacgccggc cgcatgcgcc
     2161 gcccggccgc ggcatgcgtg tgccgcacgc ccccgccttg cctcacgtcc acgtagccga
     2221 cttcgcgcac gcctttcgcc ctgctcattt cagtcattct ccccaaatgc gtgagcggta
     2281 ggcggtaagc agtaagccgt gaaatacaga ttggggatgg gtaaagtaat tctgttgata
     2341 ccgagcgtgc ggagggcggg ttttccgtct gacggcaggt tggtgtgtgg ggtaattcgc
     2401 atgcatgaac tccaaacatg cgaaatcccc ctg
//
LOCUS       DBNGWZ010000593         6615 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3239460, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000593 DBNGWZ010000000
VERSION     DBNGWZ010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3239460"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1416)
                     /locus_tag="AC16CX_18460"
     CDS             complement(214..1416)
                     /locus_tag="AC16CX_18460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3014525.1"
                     /translation="MFVNRVLSGMRPTGALHLGHYHGVLKNWIRLQHEYECLYFVADW
                     HALTTHYDTPEIIEQNVWDMVIDWLAAGVDPAQATLFIQSRVPEHAELHLLLSMVTPL
                     GWLERIPTYKDQQEKLADKDLSTYGFLGYPLLQSADILIYHANQVPVGEDQVPHVEFT
                     REIARRFNHVFGREEGFEQKAEAAIKKLGAKRARLYRQLRTKFLEKGDAEALAAARAL
                     VEEAQNLPMGDRERLFGYIEGGGKVVLTEPQALLTAAPRMPGLDGQKMSKSYDNTIGL
                     REDAESITKKIRTMPTDPARVKRSDPGDPEKCPVWQFHLVYSDDKRKQWVQQGCRSAG
                     IGCLECKQTVIDAVLEEQKPMRERAAQYLEDPTLVRSIIADGCEKARKLAQETLREVK
                     EAMGLNYT"
     gene            complement(1436..2131)
                     /locus_tag="AC16CX_18465"
     CDS             complement(1436..2131)
                     /locus_tag="AC16CX_18465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3014526.1"
                     /translation="MELNLLQIISIYALPVIFAITLHEAAHGYVAKHFGDTTAYMEGR
                     VSLNPLRHIDPFGTVALPLILLAVSKLFGGGIIFGWAKPVPVNFANLRHPKRDMLWVA
                     AAGPFSNLAMALFWALMVKIGLTMQESYFTLPLMLMGAAGVFFNVIIMVLNLVPLPPL
                     DGGRILVSLLPHRLAWRVARVEPYGFFILLLLLFTGVLGFVLWPVIGALMALIAGLFD
                     LPVKDLIRLTGLA"
     gene            complement(2229..2852)
                     /locus_tag="AC16CX_18470"
     CDS             complement(2229..2852)
                     /locus_tag="AC16CX_18470"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR3014527.1"
                     /translation="MAQFFSIHPDNPQPRLVRRAVEIVRAGGVIVYPTDSCYALGCRL
                     GDKAAMERLRAIRQVDERHHFALVCRDLAEIAVYARVDNRQFRLLKSATPGSYTFILK
                     ATREVPKRLLHPSRRTIGLRVPDHRVVRALLDELGEPLLSSTLMLPGDDVPLNDAGEI
                     RERLEHRVDLILDCGACGITPTTVVDLTGEAPVVTRQGKGSLALFGA"
     gene            3047..3835
                     /locus_tag="AC16CX_18475"
     CDS             3047..3835
                     /locus_tag="AC16CX_18475"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR3014528.1"
                     /translation="MPRNCIRLATAIAMTLTAATAAAATCSAESGARRVALLELYTSE
                     GCNSCPPADRWVSALPERSYNPERVVTLGFHVDYWNYLGWNDPFARPEYSARQRSASQ
                     RNQARFVYTPQLLLNGRDFRRGIFKDDFDDRVTAINRDAPRARIGLKLASGAADALSV
                     NVAVAVTDLTQRMSARVFLALYENDLSSLVTAGENRGKRLRHDFVVRGLAGPHPVDTQ
                     GKAQFSHRFKLDSRWKSANLHVVAFVQNEATGDVLQALAIPHCQ"
     gene            3980..4543
                     /locus_tag="AC16CX_18480"
     CDS             3980..4543
                     /locus_tag="AC16CX_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014529.1"
                     /translation="MNRHQTIALVVAAVNLLLILLFPPVDQYSIAATKVPVFAGFHLV
                     FATPPNSVINASVLWLEVMVVLINTGIAWLLLRDKAAGTARRRLSFQNAVLIFTGANL
                     VLTLLFPPFESVFALTNATLPTFEGFYFIFFQKPSHVIVTSLLYMEAVFILVNGGLLW
                     LIFKPNTSQELAVEEARALAEAMRKKK"
     gene            complement(4624..5238)
                     /locus_tag="AC16CX_18485"
     CDS             complement(4624..5238)
                     /locus_tag="AC16CX_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3014530.1"
                     /translation="MNIAIELQAAQHGGYPLILTLDTHGVPHRWISWQHACYYYAKDQ
                     VAWTTGGHAFTVYGGMSRLTGRRSSITASSIIAIKGKAMAMKAFSQVPPLNNRELFHR
                     DRQICAYCGGMFAISRLTRDHVTPFSRGGRDTWMNSVTACRVCNERKGNCTPEQVGME
                     LIYLPYVPNRAEYLILTNRRILADQMEFLAQHVPAQSRLRQKVA"
     gene            5477..>6615
                     /locus_tag="AC16CX_18490"
     CDS             5477..>6615
                     /locus_tag="AC16CX_18490"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3014531.1"
                     /translation="MQDAPSSPKPSFPALGLIPELLRAVAEQGYSEPTPIQVKAIPPI
                     LQRRDVMGAAQTGTGKTAGFTLPLLQLLAPQANTSLSPARHPIRVLILTPTRELAAQV
                     EESVRTYGKHLPLRSAVVFGGVDIDPQMKQLHAGVEILVATPGRLLDHLHQKSVNLSR
                     VEFLVLDEADRMLDMGFIPDIKRILAVLPPRRQNLLFSATFPDEVRRLAKGLLRAPVT
                     IEVAPRNAPPELVTHLVYPVPVPRKRALLVHLIRSRDMRQVLVFVRMKRDANRLARQL
                     VHDGVAAKEIHSDRTQAERMQALEDFKQGKVPVLVATDIAARGLDIEELPFVINFELP
                     HAAEDYVHRIGRTGRAGLPGEAISLVSPDEMKFLEDIERLLKRKLP"
BASE COUNT         1254 a         2085 c         2023 g         1253 t
ORIGIN      
        1 cgagggactc ctcagtcatg agaaacaccc tcccggcagg agtaggaccc ggccggagcc
       61 tgcggaaatt tgggggggaa gtcgcctgcc ggctggggga agccgccggt cattgcgtcc
      121 ggatcagagg ccagaaggcc accgcccggc gtaggcgatg ctggcatgta gttattctac
      181 aggcttttcg cagcaaaatc agcctttttc ggtttatgtg tagttcagcc ccatcgcctc
      241 cttcacttcg cgcagcgtct cctgcgccag cttgcgcgcc ttctcgcaac cgtcggcgat
      301 gatgctgcgc acgagcgtgg gatcctccag atactgcgcc gcccgttcgc gcatcggttt
      361 ctgctcttcg agcacggcgt cgatgaccgt ctgcttgcac tccaggcagc cgatgccggc
      421 gctgcggcag ccttgctgca cccactgctt gcgcttgtcg tccgaataca ccaggtggaa
      481 ttgccagacc gggcatttct ccgggtctcc tgggtcggaa cgcttcaccc gtgccggatc
      541 ggtcggcatg gttcgtatct tcttggtgat gctctcggcg tcctcgcgca ggccgatggt
      601 attatcgtac gacttcgaca tcttctggcc gtccagtccc ggcatcctgg gggccgcggt
      661 gagcagcgcc tgcggctcgg tcagaaccac cttgcccccg ccctcgatgt agccgaacag
      721 ccgctcgcgg tcacccatgg gcaggttctg cgcctcctcc accagcgcgc gcgccgcagc
      781 gagcgcttcc gcgtcgccct tctccaggaa cttggtgcgc agctggcgat agagacgggc
      841 gcgtttagcg ccgagcttct tgatcgctgc ctcggccttc tgctcgaaac cttcttcccg
      901 cccgaacacg tggttgaagc ggcgggcgat ctcgcgcgtg aactcgacgt gcggaacctg
      961 atcctcgccc accgggacct ggttggcgtg gtagatcagg atgtccgcgc tctgcagcag
     1021 gggatagccc aggaagccgt aggtggagag gtccttgtcg gcaagctttt cctgctggtc
     1081 cttgtaggtc gggatccgct cgagccatcc cagtggcgtc accatggaca gcagcaggtg
     1141 cagctcggcg tgctccggga cgcgcgactg gatgaacagc gtcgcctgcg ccgggtcgac
     1201 gcccgccgcg agccagtcga tcaccatgtc ccacacgttc tgctcgatga tctcgggcgt
     1261 gtcgtagtgg gtggtgagcg cgtgccagtc ggcgacgaaa tacaggcact cgtactcgtg
     1321 ctgcaggcgg atccagttct tgagcacgcc gtggtagtgg cccaggtgca gcgccccggt
     1381 cgggcgcatt cctgagagta cgcggttgac gaacatcgtg ttacaggctc ttggatcagg
     1441 cgaggcccgt cagcctgatc agatccttca ccgggagatc gaacaggccg gcgatcagcg
     1501 ccatcagcgc tccgatcacc ggccagagca cgaagccgag cactcccgtg aagagcagca
     1561 ggaggaggat gaagaagccg tagggctcga cacgcgcgac gcgccaggcc aggcggtgcg
     1621 gcagcaggct taccaggatg cgcccgccgt cgagaggagg caggggcacc aggttcagca
     1681 ccatgatgat gacattgaag aagacgcccg ccgcccccat cagcatcagc ggcaaggtga
     1741 aatagctctc ctgcatggta agcccgatct tcaccatcag cgcccagaac agcgccatcg
     1801 cgaggttgct gaacgggccg gcggcggcaa cccacaacat gtcgcgcttg ggatggcgca
     1861 agttggcaaa gttgaccggc accggcttcg cccatccgaa gatgatgcca ccgccaaaga
     1921 gcttgctcac cgcgagcagt atcagcggca gcgcgacggt accgaacgga tcgatgtgcc
     1981 gcagcggatt caggctcacg cgcccctcca tgtaggcggt cgtgtcgccg aaatgcttgg
     2041 cgacataacc atgcgcagcc tcatgcaggg tgatcgcgaa gatcactggc agggcgtaga
     2101 tcgagatgat ctggagcaga ttcagttcca aaaaatgacg ggcgcgaaat gatgaatatg
     2161 gaatatttta tcaggcttta acggctgacc atcccgttca tcgattaccc atcatgatcc
     2221 gttccgcatt acgcgccgaa caaagcaagg ctgcccttgc cctgccgcgt gacaaccggc
     2281 gcctcgccgg taagatcgac gacggtcgtc ggtgtgatcc cgcacgcgcc gcaatcgagg
     2341 atgagatcca cccggtgctc caggcgctcg cggatttcgc cggcatcgtt caggggcacg
     2401 tcgtcgccag gcagcatcaa cgtcgagctc agcagcggct cgcccaattc gtccagcagc
     2461 gcccgcacca cgcgatggtc cggaacgcgc agcccgatcg ttctgcggct cggatgcaac
     2521 aaacgcttcg gcacttcccg cgtcgccttg aggatgaagg tataactccc gggggtggcg
     2581 cttttcagca gacggaactg gcggttgtcc accctggcgt agaccgcgat ctcggcaaga
     2641 tcacggcaga ccagcgcgaa gtgatggcgc tcgtccacct ggcggatggc gcgcaggcgc
     2701 tccatcgccg ccttgtcgcc gaggcggcaa ccgagcgcgt agcaggagtc ggtcggatag
     2761 acgatcacac cgcccgcgcg cacgatttcc accgcgcgcc ggaccagacg cggctggggg
     2821 ttgtcgggat gaatcgagaa aaattgggcc acctaaactc cgtgatgggt gatgatgagt
     2881 aatgggtgat gggtaagcgc gagaatctag tatattaaat acctttatta acaatgtatt
     2941 accagattat attcaagcat aatataagaa agcgccgagg ttgatgtcat tctgtgttcg
     3001 accagggtgg agcagccaaa gtccaatggc gcgcgtatat agggccatgc ccaggaactg
     3061 catccggctc gctaccgcca ttgccatgac cctgaccgct gccacagcag ccgctgcaac
     3121 ctgcagcgcg gaaagcggcg cgcggcgcgt ggcattgctc gaactctaca cgtccgaggg
     3181 ctgcaacagt tgcccacccg ccgaccgctg ggtaagcgct ctgccggaac gcagttacaa
     3241 cccggaacgc gtggtcacgc tcggcttcca cgtggactac tggaattacc tgggctggaa
     3301 cgatccgttc gccaggcccg aatacagcgc gcgccagcgg tccgccagcc agcgtaacca
     3361 ggcgcgcttc gtctacacgc cccagctgct gctcaacggc agggatttcc gccgcggcat
     3421 cttcaaggac gatttcgacg accgcgtaac agcaatcaac cgggacgctc cccgtgcgcg
     3481 aatcggcctc aagctcgcct ccggagcggc ggatgcattg tccgtcaacg tagcagtagc
     3541 ggtaacggat ctgacgcagc gaatgagcgc ccgggtcttt ttggcgctct acgaaaacga
     3601 tctttcaagc ctggtcacag ccggagaaaa caggggcaag cggttgcggc acgatttcgt
     3661 ggtccgcggg ctggcgggtc cccacccggt ggatacccaa ggcaaggctc aattcagcca
     3721 tcgcttcaag ctcgattccc gctggaaaag cgcaaacctg cacgtggtgg cgttcgtaca
     3781 gaatgaagcg accggtgacg tgctccaggc actggcaatc cctcactgtc agtagcggta
     3841 tgtcccgcat actttacttg cggggtaccg ggctggtgcc atgaaagcag gaccaacgtg
     3901 gatgtccagc tttcccgaaa aaggtgtttt ccccgtcagc tataatccat cgtatcgggg
     3961 aaggacaaag ggggcggcaa tgaaccgaca ccaaacaatc gccctcgtgg tcgcggcagt
     4021 caatctgctc ctgatcctgc tgttcccgcc agtggatcaa tactcgattg cagccaccaa
     4081 ggtgccggtg ttcgccggct tccaccttgt gttcgccaca ccgcccaaca gcgtgatcaa
     4141 tgccagtgtg ctgtggctcg aggtgatggt ggtgttgatc aacactggaa tcgcctggct
     4201 gctgctgcgc gacaaggctg ccggtacagc gagacgcagg ctgagttttc agaacgcggt
     4261 gctgattttc accggcgcca atctggtgtt gacgctgctg tttcccccct tcgagtcggt
     4321 atttgccctg accaacgcca cgctgccgac cttcgagggg ttctatttca tcttctttca
     4381 gaaacccagc cacgtcatcg tcacctccct gctctacatg gaggcggtgt tcattctagt
     4441 gaacggcgga ctgttgtggc tgatcttcaa gcccaatacg tcgcaggagc ttgccgtgga
     4501 ggaggcgcgc gccctggctg aggcgatgcg caagaagaaa taaagccgtc agcggtcagc
     4561 tgtcagcaag acctgaaagt tttaactgaa agctgagtgc tgaatgctga tcgccacgaa
     4621 gtgctaggcg accttttgac gcaggcggct ttgcgccggc acatgctgcg ccagaaattc
     4681 catctggtcc gcgaggatgc ggcggttggt gagaatcagg tattccgccc ggttcggcac
     4741 gtacggcagg tagatcaact ccatcccgac ctgttccggc gtgcagttcc ccttgcgctc
     4801 gttgcagacg cggcatgccg tcacggaatt catccaggta tcgcgcccgc cgcgcgagaa
     4861 cggggtcacg tggtcgcgtg tgagccggga aatcgcgaac atgccgccgc agtaggcgca
     4921 gatctgacgg tcgcggtgga acagctcccg gttgttgagc ggcggcacct gcgaaaacgc
     4981 cttcatcgcc atcgccttgc ctttgatcgc gatgatgctg gaagccgtga tgctggagcg
     5041 tctgccggtc aaccggctca tgccgccgta gacggtgaaa gcgtgccccc ccgtcgtcca
     5101 cgcgacctgg tccttggcgt agtagtagca ggcatgctgc cagctgatcc agcggtgtgg
     5161 cacgccgtgc gtgtcgagcg tcaggatcag cggatagccg ccgtgctgtg ctgcttgtag
     5221 ctcaatagca atattcatct cttgcgatcg ttccatagca cctgccaacg gtgcagtgcg
     5281 aaatttacca gatttttgaa tttgtggcag ttttgcttga tcacagcacc cttccaggca
     5341 ggatgacgga attacgttct cgtagtgcac cgcaccgcag caaggtataa ttgcgccccg
     5401 tgaatgaccg tgcagccttt ttcgagtgcc gttgccggca gttgtgacct tggttcaaga
     5461 cggcgctgcc tatcccctgc aggacgctcc tagctccccc aagccgtcct ttcccgcctt
     5521 gggtttgatt cccgagctgc tacgtgcggt cgcggagcag ggctacagcg agccgacgcc
     5581 gatacaggtc aaggcgatcc cgcccatctt gcaacgccgg gatgtcatgg gcgccgcaca
     5641 aaccggcacc ggcaagacgg cgggtttcac gctgccgctg ctccagttgc tcgcccccca
     5701 ggccaacacg agcctgtctc ccgcgcgcca tccgatccgg gtgctgatcc tcacgccgac
     5761 ccgcgagctt gcggcgcagg tcgaggaaag cgtgcgcacc tacggcaagc atctgccgct
     5821 gcgttcggcg gtggtgttcg gaggcgtgga catcgatccg caaatgaagc agctgcacgc
     5881 cggcgtggaa atcctggttg cgactcccgg acgattactc gaccacctgc accagaaaag
     5941 cgtcaacctg tcgcgcgtgg agttcctggt cctcgatgaa gccgatcgca tgctcgacat
     6001 gggcttcata cccgacatca agcgcatcct cgccgtgctg ccgccgcggc gccagaacct
     6061 gctgttctcg gcgactttcc ctgatgaggt gcgcaggctc gccaaggggc tgctgcgcgc
     6121 gccggtaacg atcgaggtcg cgccgcgcaa tgcgccgccc gagctcgtta cgcacctggt
     6181 ctaccccgtc cccgtgccgc gcaagcgagc actgctcgtc cacctcatcc ggtcgcgcga
     6241 catgcggcaa gtgctggttt tcgtccggat gaaacgcgat gcgaatcggc tcgcgcgcca
     6301 gctcgtgcac gatggcgtcg ccgccaagga gatacacagc gatcgcaccc aagccgagcg
     6361 catgcaggcg ctcgaggact tcaagcaagg caaggtgccg gtgctggtcg caaccgacat
     6421 cgcagcacgc ggcctcgaca tcgaggagct gccgttcgtc atcaacttcg agttgcctca
     6481 cgccgcggaa gattacgtgc accgcatcgg ccgcaccgga cgggctgggc tcccgggcga
     6541 ggccatctca ctggtcagcc ccgacgagat gaaattcctc gaggatatcg agcggctgct
     6601 caagcgcaag ctgcc
//
LOCUS       DBNGWZ010000594         5033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1477348, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000594 DBNGWZ010000000
VERSION     DBNGWZ010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1477348"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="AC16CX_18495"
     CDS             <1..235
                     /locus_tag="AC16CX_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014532.1"
                     /translation="DANSTTGTIFRMTAISLWHINYAVETSRIAASTGPLLVALRDQG
                     VTALQNTTAARKLSFQHDRMGELQPNNLICLLY"
     gene            347..784
                     /locus_tag="AC16CX_18500"
     CDS             347..784
                     /locus_tag="AC16CX_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3014533.1"
                     /translation="MKIDGGCHCGNISFVAEIDPEKVTICHCTDCQTFSGSAYRVFVP
                     VAKNAFKLSGGKPKTYVKTAESGSKRMQGFCPECGTSLFSGAAVDPQFFTVRIGTVRQ
                     RAELRPKKQQWCRSKLDWAMDLRDVPQIAKQLTNQEPPREARR"
     gene            1047..2129
                     /gene="queG"
                     /locus_tag="AC16CX_18505"
     CDS             1047..2129
                     /gene="queG"
                     /locus_tag="AC16CX_18505"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3014534.1"
                     /translation="MERTAERHLAKTDYADLARQIKQWGGELGFQQVGIADCEMSEAE
                     ARLLDWLGKGYHGEMEYMERHGVKRARAAELVPGTVRVIAARMNCNPPARATEEVMAD
                     GSKAFIARYTLGRDYHKIMRGRLQKLADRIKKQVRDFRYRVFTDSAPVMEVEIAQKAG
                     LGWRGKHTLLLNREAGSFFVLGEIYTDLPLPTDSVPENHCGTCQKCMDVCPTGAIVGP
                     YQLDARRCIAYLTVELKGSIPEELRPLIGNRVYGCDDCQLHCPWNKYAQTTTEPDFAV
                     RYGLDDVTLVELFGWSEAEFEKRMAGSAIHRIGYERWLRNLAVGLGNAPKSAAVVTAL
                     RARANHPSALIREHVGWALARHGVSL"
     gene            2240..3886
                     /locus_tag="AC16CX_18510"
     CDS             2240..3886
                     /locus_tag="AC16CX_18510"
                     /inference="COORDINATES: protein motif:HMM:NF021606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HIR3014535.1"
                     /translation="MSPEELDKRFAGIEQRLAFIESLIPKDAKPIAEQAAKPAAAQAA
                     KPAPPPPKPEPGDRPSLVTSILGWGGAVALVLAAAYLIRLAVESGWLTPVRQVACAAI
                     SGLVLIGAGFFMRHINRQYAGLLPAGGVAILFLSIYGAHLFYGLMPATPAASAVVFVC
                     LLSLWLCRAFESDLYALFAVAGSYSAPVLISGAPSITGLVIYFSAWGVVFSVFSIWHG
                     RRLVYLLALYLALIIFDFIWRKQAPDEWIAALVFQSIQFVVFGVATVAFSIRNKSPLD
                     SASAWGHLPPLLIFYFLEYILLKRHLPEMAPWIAVGSGFAVAGLYGVARAVLQRPLPG
                     GEFLLWAYIALVLFHAGYIEEVPHRWAPWVAFAALPVIAVATLRQGAGIGPRWPIWLA
                     AAVIFLINYLRIVFDVDLGGVPARPWLAVAYALQLYLGYYLVRARDVPQFLRGLLLYA
                     GHISAMAAALHWLDERIVESAAWGLLALACLGLSLWQRDRLVGQSSLLVFGAAAVKVM
                     LYDLGGASPMARIISLVVLGVTFYIGGLLYQRLQAAGGDK"
     gene            4000..>5033
                     /locus_tag="AC16CX_18515"
     CDS             4000..>5033
                     /locus_tag="AC16CX_18515"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014536.1"
                     /translation="MLMTRADTAQYRDEVGELIADFVVNVSSRLDARAERSVKALLID
                     SIAVAIAALRHPAAQNARKHAYRFPAGKRGCVIWGTQKRAAPELAALTNGVLLRCHDY
                     NDFFVGLRNSGHTSDILSGVIAAAEWTNAPGRKLLSAVAVGYEMVGAAFDAFSTAPGG
                     WDYTNLMQLGATAAIARLLNLNADQTREALGMTVVPHFASDEIESGELNRRGDLSMWK
                     RFNGADAVRNSLQACLLAQVGAEAAVRPFVGKCGFVRKMNMKDDPVPVLRERLDPRKP
                     LSRVNDAFMKRWPVGSVAQSAIQAALQARSRIKDISRIKQIRVFAEEGAYDHLVKIRQ
                     DPWNPISRETA"
BASE COUNT          953 a         1496 c         1670 g          914 t
ORIGIN      
        1 ggatgcaaac agcactacag gcacgatttt ccgcatgact gcgatctcct tatggcatat
       61 aaactatgct gttgagacat cccggatcgc tgcaagtacc ggaccattat tagttgcgtt
      121 gcgagatcaa ggggttaccg ctttgcagaa cacgacggcc gcgaggaaat tgtcatttca
      181 gcacgacagg atgggggagc ttcagccaaa caatttgatt tgcttgttgt attaatgtcg
      241 ccggcgagcg acagaatgca ggctgccgat tttgctggct tgcgacggca gaaaaattcc
      301 ccttctccac cgcttgcggg ggagggagtc gggtgagagg gcgatgatga agatcgacgg
      361 cggatgtcat tgcggcaaca tcagcttcgt tgcggagatc gatccggaga aggtgacgat
      421 ttgccattgc acggattgcc agaccttttc ggggtccgca tatcgcgtct tcgttcctgt
      481 ggcgaaaaac gcgttcaagt tgagcggtgg caagcccaag acctatgtca agacggcaga
      541 gagcggatcc aagcgcatgc aggggttttg tccagaatgc ggcacgtcgc tcttttcggg
      601 agccgcggtt gaccctcagt tcttcaccgt ccgcatcggc acggtgcgcc agcgcgccga
      661 gctgcggccg aagaagcagc agtggtgccg gtcgaagctc gactgggcga tggatctgcg
      721 ggacgtaccg cagatcgcca agcagctgac aaaccaggaa ccgccaagag aagcaagacg
      781 ataattgaac cgccatgacg ccaagagaaa cagaacaata atgaaccgcg gagatgggga
      841 gagaagcaag ccaatactcg aaccgccaag acgccaagag aagcaaagag aacgtaattt
      901 caatggcaag aaacatcagc cacagaaagt gacaaacgtc attccggcag aagccggaat
      961 ccaatcaatg gcaggaatgc gcgcgggcaa catcaaaata attcttcccc tcgcccccag
     1021 ctgggggaga gggtagggtg agggggatgg aaaggacagc tgagcggcat ctcgccaaga
     1081 ccgactacgc tgatctcgca cgccagatca agcagtgggg cggggagctt ggcttccagc
     1141 aagtcggcat cgccgactgc gaaatgagcg aggcggaggc gcggctgctt gactggctcg
     1201 gcaagggcta ccacggtgag atggagtaca tggagcgcca cggcgtgaag cgcgcgcggg
     1261 cggcggagct cgtgcccggc acggtgcgcg tgatcgccgc gcggatgaac tgcaatccgc
     1321 cggcgcgcgc gaccgaggaa gtgatggcgg acggctcgaa agccttcatc gcgcgctaca
     1381 ccctgggccg cgactatcac aagatcatgc gcggaaggct gcaaaaactc gccgaccgca
     1441 taaaaaagca ggtgcgcgac ttccgctacc gcgtcttcac cgacagcgcg ccggtgatgg
     1501 aagtggaaat cgcgcagaaa gcggggctcg gctggcgcgg caagcacacg ctgctcttga
     1561 accgcgaggc gggctcgttc ttcgtcctcg gcgagattta caccgacctt ccgctgccca
     1621 ccgacagcgt gccggaaaac cattgcggca cctgccagaa gtgcatggac gtgtgtccca
     1681 ccggcgcgat cgtcgggccc taccagctcg acgcgcgccg ctgcatcgcc tatctcacgg
     1741 tcgagctgaa gggcagcatc ccggaagagc tacgcccgct gatcggcaac cgggtgtatg
     1801 gatgcgacga ctgccagctc cattgcccct ggaacaagta tgcgcagact accaccgagc
     1861 ccgacttcgc cgtacgctac ggcctcgacg atgtgacgct agtcgagctg ttcggctgga
     1921 gcgaggcgga attcgaaaag cggatggcgg ggagtgcgat acacaggatc gggtatgagc
     1981 ggtggttgcg gaatttggcg gtggggttgg gaaatgcgcc gaagtcagca gcggtggtca
     2041 cggcactcag agcacgggca aatcatccat cagctcttat tcgtgagcac gttggttggg
     2101 cgctagcgcg acacggtgtg tcgctatagg gatgaggaat aagggatgag ggcggagggg
     2161 tgaggagtta aagcacaagg caaaagggtc gatccgtgag tcacgatctg cgagtcacgc
     2221 atcatcaagt gaggggagca tgagtccaga agaactcgac aagcgttttg ccggaatcga
     2281 gcagcgcctt gcattcatcg agtcgctgat cccgaaggac gccaagccga tagcggagca
     2341 agcggcaaaa ccggcagcgg cccaggcggc aaaaccggcg ccgccgccgc cgaagcctga
     2401 gccgggagac cgcccgtcgc tcgtcaccag catcctcggc tggggcgggg cggtcgcgct
     2461 ggtgcttgcg gcggcctatc tcatccggct ggcggtcgag agcggatggc tcacgccagt
     2521 gcggcaggtt gcgtgcgcag ccatctcggg attggtgctg atcggcgcgg gattcttcat
     2581 gcgccacatc aaccgccagt acgcgggatt gctgccggcg ggcggcgtgg ccatcctgtt
     2641 cctctcgatc tacggcgcgc atctcttcta cggactcatg cccgccacgc ccgcggcttc
     2701 ggcggtggtt ttcgtctgcc tgctgtcgct ctggctgtgc cgcgcgttcg aaagcgacct
     2761 ttacgccttg ttcgcggtgg cgggctcgta ttccgcgccg gtattgatct ccggggcgcc
     2821 gtcgatcacg ggcctcgtga tctacttctc ggcgtggggc gtcgtgttca gcgtcttctc
     2881 catctggcac ggccggcgcc tggtgtattt gctggcgctc tatcttgcgc tgatcatttt
     2941 cgatttcatc tggcgcaagc aggcgcccga tgaatggatc gccgcgctcg tgtttcagtc
     3001 gatccagttc gtggttttcg gcgtggccac ggtcgcattc tcgatccgga acaagtcgcc
     3061 gctcgattcc gcgtcggcgt ggggccactt gccgccgctc ctgatcttct actttctcga
     3121 atacattctg ttgaagcggc acttgccgga gatggcaccg tggatcgcgg tgggcagcgg
     3181 attcgctgtc gcgggccttt acggcgttgc ccgcgcggtc ctccagcgcc cattgcccgg
     3241 cggcgaattc ctgctatggg cgtatatcgc gctggtgctg tttcacgccg gctatatcga
     3301 ggaggttccc catcgctggg cgccatgggt ggcattcgcg gccctgcccg tcatcgctgt
     3361 ggcgacgttg cggcagggtg cgggtatcgg gccaaggtgg ccgatctggc tcgcggccgc
     3421 ggtgatcttc ctgatcaatt acctgcgcat cgttttcgac gtggacctcg gcggggtccc
     3481 ggcccggccg tggctcgcgg ttgcctacgc gctgcagctc tacctgggct attacctggt
     3541 ccgggcgcgc gacgtgccgc agtttctgcg gggattgctg ctttacgccg gccacatcag
     3601 cgcaatggcc gcggcccttc attggctcga cgagcggatc gtcgagtccg ccgcctgggg
     3661 gctgctcgcg ctcgcgtgcc tcggcctttc gctatggcaa cgcgaccgat tggtggggca
     3721 gtcctcgctg ctcgtgttcg gcgccgcggc ggtcaaggtc atgctgtacg acctgggcgg
     3781 cgcgtctccc atggcgcgga tcatcagcct cgtggtgctg ggcgtcactt tttatatcgg
     3841 cggattgctt taccagcgcc tgcaggcagc gggaggtgac aaataggagc agcgcagccg
     3901 tcccctattt gacccaggga taaggaatga gggatgaggg cggagggatg agcgagacga
     3961 cccacgaccc acgacccacg agcggagtgt aggacacgta tgttgatgac ccgcgcggat
     4021 accgcgcagt atcgcgatga agtcggagag ttgatcgccg atttcgtggt gaacgtctcc
     4081 agccggctcg acgcgcgtgc cgagcgctcc gtcaaggccc tcctcatcga ttccatcgcc
     4141 gtcgcgatcg ccgcgctgag acatccagcc gcgcagaacg cgcgaaagca cgcctaccgc
     4201 ttcccggcgg gcaagcgcgg ctgcgtgatc tggggaacgc aaaaacgcgc ggcgcctgag
     4261 ctggccgcgc tgacgaacgg cgtgctgctg cggtgccacg actacaacga tttcttcgtg
     4321 ggcctgcgca acagcggcca tacgagcgac atcctctccg gcgtgatcgc ggccgccgaa
     4381 tggaccaacg cccccggaag gaaactgctc tccgcggttg ccgtcggcta cgaaatggtg
     4441 ggggccgcat tcgatgcgtt ctcgaccgcg ccgggcggct gggattacac gaacctgatg
     4501 cagctcggcg cgaccgccgc gatcgcgcgg ctgctcaacc tgaacgccga ccagacgcgc
     4561 gaagcgctgg gcatgaccgt ggtcccccat ttcgcgagcg atgagatcga atcgggcgag
     4621 ctgaaccggc gcggcgacct ctcgatgtgg aagcgcttca atggagccga cgcggtacgc
     4681 aattcgctcc aggcctgcct gctcgcgcag gtcggcgccg aggcagcggt caggccgttc
     4741 gtgggcaagt gcgggttcgt ccggaaaatg aacatgaagg acgatccggt cccggtgctg
     4801 cgcgagcggc tcgatccccg caagccgctc agccgggtga acgacgcttt catgaagcgg
     4861 tggccggtcg gttccgtggc gcaaagcgcg atccaggccg cgctgcaggc gcgctcgcgg
     4921 atcaaggaca tttccaggat caagcagata cgcgtattcg ccgaggaggg cgcctacgac
     4981 cacctggtga agatacgcca ggacccgtgg aatccgatct cgcgcgagac ggc
//
LOCUS       DBNGWZ010000595         7370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3114592, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000595 DBNGWZ010000000
VERSION     DBNGWZ010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3114592"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="AC16CX_18520"
     CDS             complement(<1..913)
                     /locus_tag="AC16CX_18520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR3014537.1"
                     /translation="MQTRVGKYELKKVLGEGASGKVYLALDTYAGAEVALKVIDSRIL
                     EDSDLGELTRGQFMNEAMLAGKLAHPHIVSIYEAVMQEDSGYVAMEYVPGGSLARFTK
                     PEGLLPVEDVIQIGFKSCSALDYAFRQGIVHRDIKPANILVVEGTNIKVADFGAALLK
                     SVESTQDLLIGTPSYMSPEQITGAPLTHHSDMYAMGVVFYELLTGQRPFTASTMAALF
                     QKITKENAAPPSAARPSVPSQLDPILLRVLSKRPEDRYSSWADLALDIAQVGKLSVYQ
                     REVPDSDKFGVLRKSELFKQINDAQVWE"
     gene            1214..1493
                     /locus_tag="AC16CX_18525"
                     /pseudo
     CDS             1214..1493
                     /locus_tag="AC16CX_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            complement(1633..2601)
                     /locus_tag="AC16CX_18530"
     CDS             complement(1633..2601)
                     /locus_tag="AC16CX_18530"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014538.1"
                     /translation="MKVFFFFVAVVLSLALPGEVRAQTYPSKPVRMLVGSNVGSGPDA
                     TIRAVAGPLSDMWKQQVVVENRPSPGGVAAAEIVAKATPDGHTLLMCSVATHGIGPVL
                     NKKLPYDYIKDFTAISRIGAVPNALIVNPSLPAKSVKEFVAHAKTNPGKIQYGSSGVG
                     NSPHLSMELFRSMAGINLVHVPAAPGRLAPQEVASGGVMAAFSNLPNVIPLAKTGKVR
                     ALAVTSAKRSAQLPEVPTMIESGFAGFEVTVWSGVCAPAGLPKTIVSKINADLGKVLS
                     LPDTQKRFAEQGAEVTSSTPDQFAAFIKAEYARWAKAAKSAGVEPK"
     gene            complement(3193..4203)
                     /locus_tag="AC16CX_18535"
     CDS             complement(3193..4203)
                     /locus_tag="AC16CX_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3014539.1"
                     /translation="MKLDIFNHIFPKRFYDRMLAVLPDGRDMNKRVRAIPSIVDLDAR
                     FRIMDTFPDYAQVICLPQPPIESLGPPPVARELAKLGNDGMAELVAKHPQRFPAFIAA
                     LPMNDPDGLLEEAERAITQLGAVGVQAFTNVNGAPLTATQTMPLFDLMAKHDLPIWLH
                     PARGADTPDYKTERKSHYEIWWTLGWPYETSVAMAHMVFAGLFDKYPDLKVITHHLGA
                     MIPYCEGRVGPGWDQLGTRTSDEDYFALLKKLKRRPLDYFHMFYADTAVFGSMPATVC
                     GLEFFGADRVLFASDSPFDPEKGSAYIRWTIEIIDRLDITAAQRAAIYEGNARRLLRL
                     AA"
     gene            complement(4214..5242)
                     /locus_tag="AC16CX_18540"
     CDS             complement(4214..5242)
                     /locus_tag="AC16CX_18540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3014540.1"
                     /translation="MRATPASAMGMKASSYRLRDLAPADLDEVVAIDAGLMGRRRRLY
                     FQKRLEAALRQPNLHAQFAAVNDGILAGYVLARRLAGEFGRAEPALLLEVISVRSDCG
                     GQGIGRQLLGEIEAWGRHRALRKLRTSATWRDHDMLRFLDRAGFELDRDQVLHCAVHA
                     GPIGELEGVAGSLDEGRTPETDYSVPAADDFERLARDRVEVRLLAGHDLPAVLAIDQR
                     VTGRDREGYLTGVLHEALADSAVRVSLVARPQGVTAGFLMAKTDFGDFGRTEPVAVID
                     TIGVDPEFAGRGLGTAMLSQLFLNLNALRIEHVETVVARENPDLLAFFYRAGFAPSNR
                     LVFVKDCS"
     gene            complement(5239..5742)
                     /locus_tag="AC16CX_18545"
     CDS             complement(5239..5742)
                     /locus_tag="AC16CX_18545"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3014541.1"
                     /translation="MEFQFHITRRLHDEHEAVFSLLTRVRRAFGGPEAAAMPDLTGAA
                     WASLLRDFRIALETEIAGHFDLEERALFPLLVEAGAGDLASILNEEHAMIRSVAAPLI
                     GLLRKFRDAGLDAADWRSVRALGLELAERLDAHARKEEVAMLPELDAVLDAEQDRELA
                     ASYADGR"
     gene            complement(5795..6598)
                     /locus_tag="AC16CX_18550"
     CDS             complement(5795..6598)
                     /locus_tag="AC16CX_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3014542.1"
                     /translation="MPDSQPPRRGGRRSGTQSIQRAALLVRLIASRSRTGSRLADVVQ
                     HSRLERSTVRRMLKCLIEEGFVRQDADTRRYALGPLVFELGLAAAPQFNLVDICRPAL
                     ARLAEATGDTVFLTVRSGYDTVCIDRKEGSFPIRALTLDVGTRRPLGAGAGGLALLMP
                     LPDAEVNAIVNANAVRLRGYNNLTVPTLNGMLKRARELGYALNDNHITPGATSIGLPI
                     ISRYGQPFAAISVGAISSRMDAGRQRKLAAMLRREVRIVESALSEATRA"
     gene            6855..>7370
                     /locus_tag="AC16CX_18555"
     CDS             6855..>7370
                     /locus_tag="AC16CX_18555"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF045191.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3014543.1"
                     /translation="MDDHAGVALARSATKDITGLRSTLSWLRAQGYLIETEKEVNPDL
                     EITGLQKHFDGSFPILFNKVRGKPHARAVTNLFSDRRVVDHLFGFEGSRDRTRRIAHA
                     INHPLKPVVIEPKSAPCQEHVILDDLDVNKWITAIRHTALEPELTVGSGIRCVTGAYF
                     DGGSDLGYNRMN"
BASE COUNT         1389 a         2352 c         2256 g         1373 t
ORIGIN      
        1 gttcccagac ttgcgcgtcg ttgatctgtt taaagagctc gctcttcctg agaacaccga
       61 acttgtcgct gtccggaacc tcgcgctggt agacgctgag cttgccgacc tgcgcaatgt
      121 ccagcgcgag atcggcccag gaggagtagc ggtcctcggg ccgcttgctc aaaacccgta
      181 ataggatcgg gtcgagctgg gatggaacag acggacgtgc ggcgctcggc ggcgcggcat
      241 tctctttcgt gatcttttga aacagcgctg ccatggtcga ggcggtaaac gggcgctggc
      301 ccgtgagcag ctcgtagaac accaccccca tcgcgtacat gtcgctgtga tgggtgagcg
      361 gcgccccggt gatctgctcg ggggacatat agctcggcgt cccgatcagc agatcctggg
      421 ttgactccac tgacttcagc agcgccgccc cgaagtcggc gaccttgatg ttggtgccct
      481 caacgaccag gatgttcgca ggcttgatgt cccggtgcac gatcccctgc cggaacgcat
      541 aatccagggc gctgcaggac ttgaacccga tctggatcac gtcctccacc ggcaggaggc
      601 cctcgggctt ggtgaaccgg gcgaggctgc cccctgggac atactccatc gccacatagc
      661 cggaatcctc ctgcatcacc gcttcataga tggacacgat gtgcgggtga gcgagcttgc
      721 cggcgagcat cgcctcattc atgaattgcc cccgggtaag ctcccccaga tccgaatcct
      781 cgagaatgcg gctgtcgatg accttgagcg cgacctccgc ccctgcatag gtatcgagcg
      841 ccaggtacac cttgccggag gcaccttctc ccagcacttt cttcagttcg tatttaccaa
      901 ctcgcgtctg cactgtttcc tctcccgccc gggaatgcga ctcgaactcg aattaccggc
      961 ttccccggtt gttcctgaat cagcaacctg cggatcaaaa aaaatcacga cgtcacgatg
     1021 aggcctgggt cggtactgat caggctccgg caacaccttc atttttgggg cgtggtgaag
     1081 taatcaatca attactcact cacggataag agacagtcgt gacgcttgcc acatttttta
     1141 tttgactctt tgcccaactt gctgtctacg tgaattatgt cacttgcaat gtgtgaacaa
     1201 tgtcacacta ctggcatccg atctggtcca gagaacctta agagccatgg ggcccaacgt
     1261 gctgggggtg gccgacaatc gcctacatct cgacgcaggc caggttcctc cacctagtcg
     1321 tcgtgctgga tgcctttagc cgccgaattg tcagatgggc gatcgctgag cacctgctca
     1381 ccgagctagt gctcgccgcg ctggacatgg cgttggcaca gcgccgcccc aatggggtaa
     1441 ttcaccactc cgaccagggc acccaataca cctccatcgc cttcgggctg cgcagtaatc
     1501 ggggaccaca gtttcagcgc ccgattatcg gaaaaaacgt ggtctgtccc cgattaatcc
     1561 gcccgattaa atcccgatta aatcacggac gaaatcgacc agggccttga gtgtctcgtc
     1621 atcaagctga gactattttg gctccacgcc ggcacttttc gccgctttcg cccaccttgc
     1681 gtactcagcc ttgataaatg ccgcgaactg atcgggagtc gaggaggtaa cttcagctcc
     1741 ctgctccgca aaccgcttct gcgtgtccgg caggcttaac accttgccca gatcggcgtt
     1801 gattttcgag acgatcgtct tcggcaaccc ggccggggca catacccctg accacaccgt
     1861 gacctcgaaa cccgcaaatc cggactcgat catggtcgga acctcgggca gttgcgcgct
     1921 gcgcttggcc gatgtaacgg caagtgcccg cactttgcca gtcttggcca agggaatcac
     1981 gttgggcaaa tttgagaatg ccgccataac gccgccgctc gcgacctcct ggggcgccag
     2041 tcggccgggc gccgccggca catggacgag gttgataccc gccatggatc gaaatagctc
     2101 catggaaaga tgcggcgagt tgccaacgcc cgatgagccg tattgaattt tcccggggtt
     2161 cgtcttggca tgggcgacaa actccttgac cgatttcgcc ggtagcgatg ggttcacgat
     2221 cagggcgttg gggaccgctc cgattctcga gatggcagta aagtccttga tgtagtcgta
     2281 cggaagcttc ttgttcagca ccggaccgat cccgtgcgta gctacggaac acatgaggag
     2341 cgtgtgcccg tcgggcgtgg ccttcgcgac gatctccgcg gcagcaacgc cgcccgggct
     2401 tggccggttc tcaaccacga cctgctgttt ccacatatcg ctcagtggac cggcaacggc
     2461 gcggatggtg gcatcgggtc cacttcccac attcgatccc acgagcattc gcaccggttt
     2521 actggggtag gtctgagcgc gtacttcccc gggtaaagca agtgaaagca ccaccgcaac
     2581 gaaaaagaaa aatactttca tgacgatccc cctttcagct aaccaagctt cgggcataac
     2641 ctagttcgtt gataaactgg cagatcacgc tacgtgcgtc gcacgacgat aaagccgatt
     2701 cgattcctaa tcgagtttct cagccgatcc tcttagcggc tgctgaaaac caatgcaggc
     2761 tgtccgcaac cgccgcagcg agcacgccag tgttcaatcg cttcacgacg ttagcctggc
     2821 cgccaatcgt gcatgctgcg ggagtgccgg acggctgctt tgacgtggat caaaagcagc
     2881 ttagataagg cggaaaccgg ggcgcttttc agtcgggcag tcgcagggaa cagcaccggc
     2941 taggtcgttc gtccgacagc ctaaaagatt gctgcgctgc ggcgccggca ctggcgcggt
     3001 gctatgctgt ttggatagtg gtctgtgtaa gtgctattgc cacgtacgaa ctcgggcgcg
     3061 aattaacgac ttgtcttgac ccagcgtcgg acgaatactt cgctggccct tcccgagccg
     3121 aaccggttcc taattcggtg aacgtccggt agtggccgac tgcggacgga cgcttcacct
     3181 cgtcgccggc gatcaggcgg cgagcctgag caggcgccgc gcgttgcctt catagatcgc
     3241 tgctcgctgc gctgctgtga tgtcgaggcg gtcgatgatt tcgatcgtcc agcggatgta
     3301 ggcggagcct ttctcggggt cgaagggcga gtccgaggcg aacagcacgc ggtcggcgcc
     3361 gaagaactcg agcccgcaga ccgtcgccgg catcgagccg aacaccgcgg tgtcggcgta
     3421 gaacatgtga aagtagtcga gcgggcgccg cttcaatttc ttcagcagcg caaagtagtc
     3481 ctcgtccgag gtacgcgtgc cgagctggtc ccaccccggg ccgacgcgac cctcgcaata
     3541 ggggatcatc gcgcccagat gatgcgtgat gaccttgagg tccgggtatt tgtcgaacag
     3601 gccggcgaac accatgtgcg ccatcgcgac gctggtttcg tacggccagc ccagcgtcca
     3661 ccagatctca tagtggctct tgcgctcggt cttgtagtcc ggagtatccg ccccgcgcgc
     3721 cggatgcagc cagatcggca ggtcgtgctt cgccatcagg tcgaacagcg gcatcgtctg
     3781 cgtcgcggtg agcggcgcgc cattgacgtt ggtgaacgcc tgcacgccga ccgcgccgag
     3841 ctgcgtgatc gcgcgctcgg cttcctcgag caggccgtcg gggtcgttca tcggcagcgc
     3901 cgcgatgaat gccggaaagc gctgcggatg cttcgccacc agttccgcca tgccgtcgtt
     3961 gccgagcttc gccagctcgc gcgccaccgg cggtggcccc agcgactcga tcggcggctg
     4021 cggaaggcat atcacctggg cgtagtcagg gaaggtgtcc atgatccgga agcgcgcgtc
     4081 gagatcgacg atcgaaggaa tggcgcgcac gcgcttgttc atgtcgcggc cgtccggcag
     4141 caccgccagc atgcggtcgt agaaccgctt cggaaagatg tggttgaaga tatcgagctt
     4201 catcgtgttt ctcctaggaa cagtccttca cgaacaccag ccggttgctc ggtgcgaaac
     4261 cggcccggta aaagaacgcg agcagatcgg ggttctcgcg cgcaaccacc gtttctacat
     4321 gctcaatgcg cagcgcgttc aggttgagga atagctggga gagcatggca gtgccgagac
     4381 cgcggccggc gaactccggg tcgacgccga tggtgtcgat taccgccacc ggctcggtgc
     4441 ggccgaaatc gccgaagtcg gtcttcgcca tgagaaagcc cgcggtcacg ccctggggcc
     4501 gcgcgacgag cgacacgcgc acggcagaat ccgcaagcgc ctcgtgcagc acgccggtca
     4561 gatatccctc gcggtcgcgg ccggtgacgc gctggtcgat cgcgagaacg gccggcaaat
     4621 cgtgcccggc aagcaaccgc acctccactc gatcgcgtgc caggcgctcg aagtcgtcgg
     4681 ccgcgggcac gctgtaatcc gtttccggag tccgcccctc gtccagggag ccggcgacgc
     4741 cctccagctc gccaatcggg ccggcgtgca cggcacagtg gagcacttga tcgcggtcga
     4801 gctcgaaccc cgcgcggtcg agaaaacgca gcatgtcgtg atcacgccag gttgcggaag
     4861 tgcgcaattt gcgtagcgca cggtggcgtc cccaggcttc gatctcgccc aacagctgcc
     4921 tgccgatgcc ttgacccccg caatcggaac gcacgctgat cacttccagc agcagcgccg
     4981 gctcggcgcg gccgaactcg cccgcgagcc gcctggccag cacgtatccg gccaggattc
     5041 catcgttgac ggcggcaaac tgggcatgca ggttcggctg tcgcagtgcc gcctcgagcc
     5101 gcttctgaaa gtacagcctg cgccggcgtc ccatcaggcc ggcatcgatc gcgacgacct
     5161 cgtcaaggtc ggccggcgcg aggtcacgca agcgatagga tgaagccttc atgcccatgg
     5221 cgctcgccgg cgtcgccctc acctgccgtc cgcgtaggac gcggcgagct cgcggtcctg
     5281 ctcagcgtcg agcaccgcgt ccagttccgg cagcattgcg acctcctcct tgcgcgcgtg
     5341 agcgtcgaga cgttcggcga gctccaatcc gagcgcgcgc acggagcgcc agtctgccgc
     5401 atccaggcct gcgtcgcgga atttccgcag caacccgatg aggggcgcgg cgacactacg
     5461 gatcattgcg tgctcctcgt tgagtatgct ggcgaggtcc cccgcgcccg cctcgaccag
     5521 aagcggaaac agcgcacgct cttcgagatc gaagtgcccc gcaatctcgg tctcgagcgc
     5581 gattcggaaa tcgcgcagca agctcgccca cgcagcgccc gtcaggtccg gcatcgcggc
     5641 ggcttccggg ccgccgaatg cccgccttac gcgcgtgagc aggctgaata ccgcttcatg
     5701 ctcgtcgtgc aagcggcgtg tgatgtgaaa ttgaaattcc aaggtgagag tcctgaatcg
     5761 tgccgcgcga tgccacgcgg cgttctcctc gcgattacgc ccgggtcgct tcgctcaagg
     5821 cgctttccac gattctgact tctcttcgca acatcgccgc gagttttctc tgccgcccgg
     5881 catccatgcg gctcgagatc gcgccgacgc tgatggcggc aaacggctgg ccgtagcggc
     5941 tgatgatggg caggccaatc gaggtcgcgc caggcgtgat gtggttgtcg ttcaacgcgt
     6001 agcccagctc ccgggcgcgc ttcagcatgc cgttgagagt gggcacagtc aggttgttgt
     6061 agccgcgcag ccggacggcg ttggcgttca cgatcgcgtt gacttccgcg tccggcagcg
     6121 gcatcagcag ggccaggccg ccggcgcccg cgccaagggg gcgccgggta cccacgtcca
     6181 gcgtcagcgc cctgatcgga aaggacccct ccttgcgatc gatgcacacg gtgtcgtagc
     6241 cgctgcgaac cgtgaggaac accgtgtcac cggtggcttc ggcgaggcgc gcgagcgcgg
     6301 gacggcagat atcgacgagg ttgaactgag gggcggccgc gagcccgagt tcgaacacca
     6361 ggggtccgag cgcataacgg cgcgtgtccg catcctgcct cacgaatcct tcctcgatca
     6421 ggcacttgag catgcgccgc acggttgaac gctcgagccg ggaatgctgc actacgtcag
     6481 ccaggcgcga acccgttcgg ctgcgcgagg cgatcagccg caccagcagc gcggcgcgct
     6541 gaatgctttg agtgccggag cggcgcccgc cgcgcctcgg gggctggctg tcgggcatca
     6601 acgcattaat gcgcgcttcg ggcatatgag cacaatgtgg aacaactttt gtcttgaggc
     6661 tggcagcgat cgccaaatgc aatggtatat tagcacggtc ctgagcgcaa tccgcgatga
     6721 gaaaggctta ttcggaagca ggcgataggc tcacaatgtg gctagatagg gagtgaggcg
     6781 cattctcaat tgcgcgccgt ggctccatgc catgcgcctg ctacagacca tcaaccagac
     6841 tgggagacac cccgatggat gatcatgccg gagtggcact ggcaaggagc gcgaccaagg
     6901 acatcaccgg cctgcgctcg acgctttcct ggctgcgcgc ccaaggctat ctgatcgaga
     6961 ccgaaaagga agtcaatccc gatctcgaga tcaccggcct gcagaaacat ttcgacggca
     7021 gctttccgat cctgttcaac aaggtcaggg gcaagcccca tgcgcgcgcc gtcaccaatc
     7081 tgttcagcga caggcgggtc gtcgaccacc tgttcggctt cgaggggtcg cgcgaccgca
     7141 cgcgcagaat cgcgcacgcc atcaaccacc cgctgaagcc ggtggtgatc gaaccgaaga
     7201 gcgcgccgtg ccaggagcac gtgattctcg acgacctcga cgtcaacaag tggatcacgg
     7261 cgatccggca cacggcgctc gagcccgaac tgaccgtcgg ctcgggcatc cgctgcgtca
     7321 ccggcgcgta tttcgacggc ggcagcgacc tcggctacaa ccgcatgaac
//
LOCUS       DBNGWZ010000596        10425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1853014, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000596 DBNGWZ010000000
VERSION     DBNGWZ010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1853014"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16CX_18560"
     CDS             <1..384
                     /locus_tag="AC16CX_18560"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3014544.1"
                     /translation="FGIVKKNGILQIDYTNTLRAQGMERERAILEANRARLRPILMTT
                     LMLVFGMLPIALGQGPGAAARASMAKVIIGGQSLSLLITLLITPVSYSLLDDLSKAKF
                     ERAWAGSLQEGLKARLAYLRGHFFH"
     gene            complement(432..1931)
                     /gene="pap"
                     /locus_tag="AC16CX_18565"
     CDS             complement(432..1931)
                     /gene="pap"
                     /locus_tag="AC16CX_18565"
                     /EC_number="2.7.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HIR3014545.1"
                     /translation="MFESAELEHKIDKATYRREAPKLREALLNAQYDLKENGRFPVLI
                     LIAGVEGAGKGETVNLLNEWMDPRHIFTHAFPEPSDEERERPPLWRFWRALPPKGKIG
                     IFFGAWHTYPIVARVQGVIDEAEFSQHIAEILRFEKMLSDEGVLLLKYWFHLSKSQQK
                     ARLKALEKDPDTRWRVTDIDWKYFKLYKKFVRICEPFLRRTSTGETPWIVVPGADPRY
                     RALSFGRHLLAAMRERLDEKPVERLPDKNPPMLPAVDRRDVINSLQLDQATTKDEYEK
                     ELEKWQGRLNLASRHPRFKRISVVAVFEGNDAAGKGGAIRRVTQALDARGYHNVSVAA
                     PTEEERAQPYLWRFWRHIPRLGRFTIFDRSWYGRVLVERVEGFCKEPDWMRAYKEIND
                     FEQALVRQNIVVVKFWLATGKDEQLKRFKLREKVAFKRFKITEEDWRNRKKWAAYEQA
                     VCDMVDRTSTSIAPWTLVEANNKYHARIKVLKTLCQAMETALDRIKRKA"
     gene            complement(1965..2408)
                     /locus_tag="AC16CX_18570"
     CDS             complement(1965..2408)
                     /locus_tag="AC16CX_18570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR3014546.1"
                     /translation="MDLILWRHADAESGVPDEERKLTAKGEKQAERMAAWLKDNLPKD
                     MVVLASPARRACDTAQALTKKFEAIREVGTTANAQAVLKAAGWPRGERTVVVVGHQPT
                     LGQAAALALTGKASDWSIKKGAIWWLVARSPNEVVVRAVIAPDMV"
     gene            complement(2568..4115)
                     /locus_tag="AC16CX_18575"
     CDS             complement(2568..4115)
                     /locus_tag="AC16CX_18575"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR3014547.1"
                     /translation="MNPLSPSVELELKLQVPPGAMRRLSAHRLLKGAVPASKRRLYSI
                     YFDTPGLDLWRQGIALRLRREGGRWFQAVKGGGTALAGLHRRLEDEAEVAGPTLDFSR
                     IGDHELAGAFSSDRLRARLRPVFITRFVRSSRLLGIDSETRVEASIDRGAIKSGKRGE
                     LISELELELKGGAPQHLYEIALKLVKDLPLSLENRSKAERGYALYRGKNPKPVKAHPA
                     ALTPALSVGASFKAVMRANLAQLQANERGMLEGRDPEYLHQMRVALRRLRSAFGVFEP
                     VLSEAAVAPLSAELKWLATRLGRARDWDVFVTETLPPIEKEFGTHGELKAFSEHCRKL
                     RSDAGASARRVVRSARYRRLMLSLGGWLAAEGWLSRLEPDQRTALDAPVSGFAAKVLE
                     RRFNQVRKRGRKVGGLSAKELHRLRIAVKKFRYAADFFSGLYEGGPAREALKRLSRLQ
                     DMLGAINDAATVANLVAQGFDAAGDQHVPEAKGILLGWSHSRAVMLRRELKNAWKPFR
                     AAGRFWR"
     gene            complement(4291..6366)
                     /gene="ppk1"
                     /locus_tag="AC16CX_18580"
     CDS             complement(4291..6366)
                     /gene="ppk1"
                     /locus_tag="AC16CX_18580"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3014548.1"
                     /translation="MNKPRDIPELYLNRELGQLAFNRRVLALAENRALPLLERLRFLC
                     IVSANLDEFFEIRVSGLKAEIEAGSPEVGPARLRADYVFSQVAKEVHELVAVQYQLLN
                     QEILPGLEAEGIRFIRHTDFTPAQAEWVKAYFSREVMPVLTPIGLDPAHPFPRVLNKS
                     LNFALELEGKDAFGRNSGIAIVQAPRVLPRVIRLPKEIAGAEYDFVLLSSILQARMAE
                     LFAGMKMVNCYQFRATRNSELFVDEEEVKDLRTALRGELPHRQFGDEVRLEVAANCSS
                     QISDHLLAQFNLVEDDCYRVDGPVNLARLINVPDMVERPALKFPVFRPALPAQLEQPG
                     DMFSAIREGDILLHHPFQSFQPVIAFLEQAARDPAVVAIKVTVYRTGAESELMEILIG
                     AAKSGKEVTVVLELLARFDEEANINWAQRLEEVGAHVVYGVVGIKTHCKMLMVVRREE
                     ERLRRYVHLSTGNYHPRTTKLYTDFGLFTANEEFGVDVSDIFMQLTGLGRATKLKYLW
                     ESPFTLHKQILRAIQNEAQNAQNGKKGRIIAKMNALLEPETIAALYEASKSGVEIDLI
                     VRGVCALKPGIPGVSENIRVRSVIGRFLEHTRVFCFHNDGAEDVYLSSADWMDRNFFR
                     RVETCFPVLDPKLKKRVINEGLKPYLEDNCEVWEMDQEGRYQLRTPRRGANRHAAQEE
                     LLETLVRAP"
     gene            complement(6451..6726)
                     /locus_tag="AC16CX_18585"
     CDS             complement(6451..6726)
                     /locus_tag="AC16CX_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR3014549.1"
                     /translation="MPRMVNCVKLGREAEGLDFPPYPGALGRRIFDHVSKEAWKQWLE
                     QQKMLVNENRLNLADKKAREYLAQQMERHFFGGGADTAAGYVPPSRK"
     gene            complement(6993..9857)
                     /locus_tag="AC16CX_18590"
     CDS             complement(6993..9857)
                     /locus_tag="AC16CX_18590"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3014550.1"
                     /translation="MTKVPLSPSLRARLLLGGVAVNVMMLLLLIVNGISIMEDKLNER
                     TSIHLEEQKQLLNAALSVPLAAGQRDALAEILEHVRRDNGIIYLVLFDRSEKLVASAG
                     WEQSSPLPPVEAPAKKPFVGQREHVHSAMDIETAGRKIGSLHFGLSTAFMRAARADLI
                     RENLVIGGIALILSIVSTVALSYWLTRNLTRLTKASESLAAGDLNVRLPVEGGDEVGT
                     LTHAFNAMAAALQGRIKALAESEAKFTAIADYSYDCELWINQEGRLIWINPRVLDMFG
                     YTPEECLATQNFPAPFIAEADVTRTVRQIRRALRGGTGQDYEFRGKRKDGSEFWAAAD
                     WRPIYDSRGGYLGIRISIRDITQRKEAEQRLEATVVELRDAQAIQQEYLTRAQDEHAR
                     LSALLAAMDTGILFVSSDNRVVYTNPAFTRIWMIAPGTRLIGKSPQEALASSACALAR
                     PEEQGKHVLRLPREDEILGVLEIQISDGRLITQQVHAVEDVYGRPVGHLWLFQDVTRE
                     RQTADQLIYLAERDALTGLFNRHRFNEELARMIADAQRHDSRVALLFYDLDDFKYIND
                     TFGHRAGDAMLIRVAGEVAGQVRRNEIFSRLGGDEFAILVPEISDEMLRVLAERITRA
                     ISLVRFQFEGQSLRLTSSLGIAVYPDHADNAEDLIARADTAMYQAKEAGKNAWRIYRS
                     DLDSSFQMVKRLSWNDRILHALENNLMDLQFQGVYSTADRSLSHFEVLVRMRDKDDPS
                     LLLMPGQFIPVAEKSGKILEIDRWVLRESIQMLAELKSIPALAVNISGRSFDEPTLPQ
                     YIAEQLKHYGVAPRRLMVELTETSAVSDLHDAQRFIEALHQTGCGVSLDDFGTGFSSF
                     AYLKHLQVDSVKIDGLFIRNLPQEHDNQLFVKAIVTVARGLHKTTIAECVEDEETLAM
                     LKHFGVDCVQGYYLEKPRVDHPLLLNASRLRNTTLELRFT"
     gene            complement(9868..>10425)
                     /gene="argA"
                     /locus_tag="AC16CX_18595"
     CDS             complement(9868..>10425)
                     /gene="argA"
                     /locus_tag="AC16CX_18595"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-CoA as donor [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR3014551.1"
                     /translation="CEHGVRRAHLISGHIDDGLLLELFTHKGVGTMVTPDPIETLRQA
                     KIEDIGGILRLIEPLEAEGILVKRGRGRLEQEIGRFLVLEHDKLVIGCAALYPFPDER
                     AGELACLVVHPDFRNLGAGERLLNEIEGRARRQKLKRLFVLTTRAEHWFVERGFAEVG
                     VALLPSQKRLLYNYQRRSVVLVKRL"
BASE COUNT         1720 a         3457 c         3314 g         1934 t
ORIGIN      
        1 ttcggcatcg tcaaaaagaa cggcatcctg cagatcgact acaccaacac gctgcgcgcc
       61 cagggaatgg agcgggagcg agccatttta gaggccaacc gcgcccggct gcgccctatc
      121 ctgatgacca cgctgatgct ggttttcggc atgctgccga tcgcgctcgg gcaaggaccg
      181 ggagcggccg cgcgcgcctc catggcgaaa gtcatcatcg gcggccagtc gctctcgctc
      241 ttgatcacgc tcctgatcac gccggtctct tactcgctcc tggacgatct cagcaaggcg
      301 aaattcgagc gggcgtgggc cgggtcgcta caggagggcc tcaaggcccg gctggcttat
      361 ctgcgcggcc acttttttca ttgaggaagc caatgtcctc gcgcctatcc cggtaatcct
      421 tacgcggcgc gttacgcctt gcgctttatc cggtcgaggg cggtctccat cgcctggcac
      481 agggtcttca gcaccttgat gcgcgcgtgg tacttgttgt tggcctcgac cagcgtccac
      541 ggcgcaatcg acgtcgaggt gcggtcgacc atgtcgcaca ccgcctgctc gtaggccgcc
      601 cattttttgc ggttgcgcca gtcctcttcg gtgatcttga agcgcttgaa cgcgaccttc
      661 tcgcgcagct tgaagcgctt gagctgctcg tccttgccgg tcgcaagcca gaacttcacc
      721 acgacgatgt tctgtcgcac cagcgcctgc tcgaagtcgt tgatctcctt gtaggcgcgc
      781 atccagtccg gctccttgca gaagccttcc acgcgctcca ccagcacccg gccgtaccac
      841 gagcggtcga atatcgtgaa gcggccgagc cgcggaatgt ggcgccagaa ccgccacaga
      901 tagggttgcg cgcgctcttc ctcggtgggc gcggcgaccg aaacgttgtg gtagccgcgc
      961 gcgtcgagcg cctgggtgac gcgccggatc gcgccgccct tgccggccgc atcgttgccc
     1021 tcgaacaccg cgaccaccga aatccgcttg aaccgcggat gtcgcgaggc gagattgagc
     1081 ctgccctgcc acttttcgag ctccttctcg tactcatcct tggtcgttgc ctgatcgagc
     1141 tggagcgaat tgatcacgtc gcgacggtcg actgccggca gcatcggcgg gttcttgtcg
     1201 ggcaggcgct cgaccggctt ctcgtcgagg cgctcgcgca tcgcggcgag cagatgccgg
     1261 ccgaagctca acgcgcgata acggggatcg gcgcccggca ccacgatcca cggcgtctcg
     1321 ccggtggagg tcctgcgcag gaacggctcg caaattcgca cgaacttctt gtagagcttg
     1381 aaatacttcc aatcgatatc ggtcacgcgc cagcgcgtat cgggatcctt ctccagggcc
     1441 ttaaggcgcg ctttctgctg gctcttggag aggtggaacc agtacttgag cagcagcacg
     1501 ccttcgtcgc tcagcatttt ctcgaaacgc aggatctcgg cgatgtgctg cgagaactcg
     1561 gcctcgtcga tcacgccctg cacgcgcgcg acgatgggat aggtgtgcca ggcgccgaag
     1621 aagatgccga tcttgccctt gggcggcagc gcgcgccaga agcgccacag cggcgggcgc
     1681 tcgcgctcct cgtcggaggg ctcggggaag gcgtgggtga agatgtggcg cggatccatc
     1741 cactcgttga gcaggttcac cgtttccccc ttgcccgcgc cctcgacgcc tgcgatcagg
     1801 atcagcaccg ggaaacggcc gttttccttg aggtcatact gcgcgttgag cagagcttcg
     1861 cgcagtttcg gcgcctccct tcggtacgtc gccttgtcga ttttgtgctc aagttccgcg
     1921 gattcaaaca tagctcctcc ccttggctgg ctcgttcgtc cgatttacac catgtcgggt
     1981 gcgatcaccg cccgcaccac gacttcattg gggctgcggg cgacgagcca ccagatcgcg
     2041 cctttcttga tgctccagtc gctggctttt ccggttagcg ccaaggcagc cgcctggcca
     2101 agggtcggct ggtggccgac caccacgacc gtgcgctcac cgcgcggcca gcccgccgcc
     2161 ttgagcaccg cctgcgcgtt ggcggtcgtg ccgacctcgc ggatggcttc aaatttcttg
     2221 gtcagtgcct gggccgtgtc gcacgcgcgg cgtgccgggc tcgccagcac caccatgtcc
     2281 ttgggcagat tgtccttgag ccaggcggcc atgcgttccg cctgtttctc gcccttggcc
     2341 gtgagcttgc gttcctcgtc gggaacgccg ctttctgcgt cggcgtggcg ccacagtatg
     2401 agatccattc ggttacccct gatcgtctgc gatgtttgac aagatgcctg atgtcacttt
     2461 ataaatcaaa tagttatgcg cgcctccagc attctggatt gccactaaaa catattctgc
     2521 gccagcctga tcgaaacgca gtctgaccta ggtcaaacaa agcgcgttta tcgccagaag
     2581 cgcccggcgg cccggaacgg tttccaggca tttttcagct cgcgccgcaa cattaccgcg
     2641 cggctgtggc tccagccgag aagaatgcct ttcgcctcgg gcacatgctg atcccccgcc
     2701 gcgtcgaagc cctgagccac cagattcgcc accgtcgcgg cgtcgttgat cgcgcccagc
     2761 atatcctgaa ggcgggagag ccgcttcagg gcttcacgcg ccgggccacc ctcgtagagc
     2821 ccgctgaaaa aatccgctgc gtagcgaaac ttcttgaccg cgatgcgcag gcggtgcagt
     2881 tccttcgccg agagccctcc gactttgcgg ccgcgcttgc gcacctggtt gaagcgcctc
     2941 tccagcacct tcgcggcgaa cccgctcacc ggcgcatcga gcgcggtccg ctgatcgggc
     3001 tcgagtcggg agagccagcc ctcggccgcc agccaccccc cgagcgagag catcagcctc
     3061 cggtagcgcg cggagcgcac tacgcggcgc gcactcgcgc cggcgtcgct ccggagctta
     3121 cggcagtgct cgctgaaagc cttgagctcg ccgtgcgtgc cgaattcctt ctcgatcggc
     3181 ggcagcgtct cggtcacgaa cacgtcccag tcgcgcgccc gtccgaggcg ggtcgcgagc
     3241 cacttcagtt ccgccgacag cggcgcaacc gccgcttcgg acagtaccgg ctcgaacacg
     3301 ccaaaggcgg agcgcaggcg ccgcagagcg acccgcatct gatgcagata ttccgggtcc
     3361 cggccctcga gcatgccgcg ctcgttcgcc tgcagctgcg cgaggttggc ccgcatcacc
     3421 gccttgaacg acgcgcccac cgacagcgcc ggcgtgagcg cggcgggatg cgccttgacc
     3481 ggcttcggat ttttgccgcg atagagcgca tagccgcgct ccgccttgga gcggttctcg
     3541 agcgagagcg gcagatcctt cacgagcttc aacgcaattt cgtagagatg ctgcggggcg
     3601 ccgcccttca gctcgagttc cagctcggag atcagctcgc cgcgcttgcc gctcttgatc
     3661 gcgccccggt ctatgctcgc ttccacgcgc gtctccgaat cgatcccgag gagccggctg
     3721 ctgcggacaa aacgggtgat gaatacgggc ctcagccgcg cacgcaggcg atccgaggaa
     3781 aacgcccctg caagctcgtg atctccgatc cgggaaaaat ccagcgtggg tcccgccacc
     3841 tccgcttcgt cctcgaggcg ccgatgcagc ccggcgagcg cggttccgcc gcccttcacg
     3901 gcctggaacc agcgcccacc ttcgcgccgc agccgaagcg cgatgccctg ccgccacagg
     3961 tcgagccccg gcgtgtcgaa ataaatgctg tacaaccgcc gcttcgaggc gggcaccgcc
     4021 cccttgagca ggcgatgcgc cgagaggcga cgcatcgccc cgggaggaac ctgcaacttg
     4081 agctcgagtt cgacggaagg ggacaagggg ttcaccccgg ctccgccagc tggtttcgcg
     4141 gaggggaata ccggaattct agtgccgttt ccgcccggag ggatacccgg ctgcggcgac
     4201 ctgcggtcgc gcaaggagtt tgtctgaccg aggtcaaaca aactcccagc agcctatcgg
     4261 acacccacat ccgacaggct gctgggccgg ctacggggcg cggaccagcg tctcgagcag
     4321 ttcctcctgg gccgcgtggc ggttcgcgcc gcggcggggg gtgcgcagct ggtaacggcc
     4381 ttcctggtcc atctcccaca cctcgcagtt gtcctcgaga tagggcttga gcccctcgtt
     4441 gatgacgcgc ttcttgagct tcgggtcgag taccgggaaa caggtctcca cccgccggaa
     4501 gaagttgcgg tccatccagt cggcgctgga gaggtagacg tcttccgcgc cgtcgttgtg
     4561 gaagcagaat acacgagtgt gctccaggaa ccggccaatg acggagcgca cgcggatatt
     4621 ctcggagacg cccggaatgc cgggcttcag cgcgcacact ccgcgcacga tcaggtcgat
     4681 ctcgacaccg cttttcgatg cttcgtagag cgcggcgatg gtttcgggct caagcagggc
     4741 gttcatcttg gcgatgatgc ggcccttttt cccgttctgg gcgttctgcg cttcgttctg
     4801 gatcgcccgc aggatttgct tgtgcagcgt gaatggcgat tcccacaaat acttcagctt
     4861 cgtcgcgcgg ccgagccccg tcagttgcat gaagatgtcg ctcacgtcga ccccgaactc
     4921 ctcgttggcg gtgaacaggc cgaaatcggt atagagcttg gtggtgcggg ggtggtagtt
     4981 gccggtcgaa aggtgcacgt agcgccgcag ccgctcctct tctcgccgca ccaccatcag
     5041 catcttgcaa tgggtcttga tgccgacaac gccgtagacc acgtgcgcgc cgacctcctc
     5101 cagccgttgc gcccagttga tattggcctc ttcgtcgaag cgcgcgagga gttccagcac
     5161 cacggtcacc tccttgccgc tcttcgccgc gccgatcagg atctccatga gctccgattc
     5221 cgcgccggtt cggtacaccg tcaccttgat tgcgaccacc gcaggatcgc gcgcagcctg
     5281 ctcgaggaag gcgatcaccg gctggaatga ctggaacggg tgatggagca ggatgtcgcc
     5341 ctcgcggatg gcgctgaaca tgtccccggg ttgctccaac tgcgccggca acgccggacg
     5401 gaaaacgggg aacttgaggg ccggccgctc caccatgtcc ggcacgttga tgagccgcgc
     5461 caggttgacc ggtccgtcca cacgatagca gtcgtcctca accaggttga actgcgcgag
     5521 caggtggtcc gaaatctgcg acgagcagtt tgcggcaacc tcgagccgca cctcgtcgcc
     5581 gaactggcgg tgcggcagct cgccgcgcag cgccgtgcgc aggtccttca cttcctcctc
     5641 gtccacgaag agttcgctgt tgcgagtggc ccggaactgg tagcagttca ccatcttcat
     5701 cccggcgaag agttccgcca tacgcgcctg cagaatggag gaaagcagta cgaaatcgta
     5761 ttccgcgccg gcgatttcct tcggcaggcg gatgacccgc ggcagcacgc gcggcgcctg
     5821 cacgatggcg atccccgagt tccggccgaa ggcgtccttg ccctcaagtt cgagggcgaa
     5881 gttgaggctc ttgttgagca cgcgcgggaa cggatgcgcc gggtcgagcc cgatcggcgt
     5941 cagcaccggc atgacctcgc gggaaaaata ggccttgacc cattccgcct gcgccggggt
     6001 gaaatccgtg tggcggatga agcggatgcc ctcggcttca agcccgggca ggatttcctg
     6061 gttgagcagt tgatactgta cggccaccag ctcgtgcacc tccttcgcga cctggctgaa
     6121 gacgtagtcg gcgcgcaggc gggccgggcc gacctcgggg gagccggcct cgatctcggc
     6181 cttcagcccc gagacccgga tctcgaagaa ctcatcgagg ttggccgaga cgatacacaa
     6241 aaaacgcagc cgctcaagca gcggcagcgc gcggttctcc gccagcgcca gcacgcgccg
     6301 gttgaacgcg agctggccga gctcgcggtt gaggtacagc tctggaatgt cgcgtggctt
     6361 gttcatcgcg tcgcgctggc gcggagccgc cgcgccgctc gcggcgcggg cgtcagtccg
     6421 cagtcctgtc agtcgggtgg tgcgtagcgc ctatttcctg gaaggtggaa cgtaaccggc
     6481 ggccgtatcc gccccgccgc cgaagaaatg cctttccatc tgctgcgcaa gatactcgcg
     6541 cgctttcttg tccgcgaggt tgagccggtt ttcattgacc agcatcttct gctgttcgag
     6601 ccactgcttc caggcttcct tcgagacatg gtcaaagatc cgccgcccga gcgcgcccgg
     6661 atagggcgga aaatcaaggc cttccgcctc gcgcccgagc ttgacgcagt tcaccattct
     6721 aggcatggcc ggcctttcag gctagcgtcg ggatgggtcg gaaatattgg ggaaatgtta
     6781 cggccacccc aggaatttgt catgcctgcg ctcgacaaat tccttaatct gaatatttca
     6841 tgaagccggt tggcgtatct ggctggccgt gttccatcag gtacttggaa ccgcgttgct
     6901 agaaaaacaa ttaagcccgc atgtcgcagg tgcttccacg tgccgctcga tcggatgagc
     6961 cccaaggcca tgacagtcat gaaacatgcg ggctaggtaa atcgcagctc cagcgtcgtg
     7021 ttccgcaggc gcgatgcgtt cagcaacagg ggatgatcga cccgcggctt ctcgagataa
     7081 tacccttgca cgcagtccac gccgaaatgc ttcagcatgg cgagtgtttc ctcgtcctcc
     7141 acgcactcgg cgatggtggt cttgtgcagt ccccgggcga cggtcacgat cgccttcacg
     7201 aacagctggt tgtcgtgctc ctggggcaga ttgcggatga acaggccgtc gatcttcacc
     7261 gaatccacct gcaggtgctt cagataagcg aaggacgaga agcccgtgcc gaagtcatcg
     7321 aggctgacgc cgcacccggt ctggtgcagc gcctcgatga agcgctgggc gtcgtgcagg
     7381 tcggagaccg cggacgtctc cgtgagctcc accatcagcc gccgcggggc gacgccgtaa
     7441 tgcttcagct gctccgcgat gtactgcggg agagtcggct cgtcgaacga gcgaccggag
     7501 atgttgaccg cgagcgccgg gatggatttg agctcggcca gcatctggat ggattcgcgc
     7561 agcacccagc ggtcgatctc gagaatcttg cccgacttct ccgccacagg gatgaactgt
     7621 ccgggcatca gcagcaacga ggggtcgtcc ttgtcgcgca tgcgcaccag cacctcgaag
     7681 tgcgagagcg agcggtcggc ggtggaataa accccctgga actgcaggtc catcaggttg
     7741 ttttccagcg cgtgcaggat gcggtcgttc caggacagcc gcttcaccat ctggaagctg
     7801 ctgtcgagat cgctgcggta gatgcgccag gcgttcttgc ccgcctcctt ggcctggtac
     7861 atcgcggtgt cggcgcgcgc gatcaggtcc tccgcgttgt cggcgtggtc ggggtagacc
     7921 gcgatgccga gcgagctcgt gagccgcagg ctctggccct cgaactggaa gcgcaccagc
     7981 gagatcgcgc gcgtgatgcg ctcggcgagc acgcgcagca tctcgtcgga gatctccggc
     8041 accaggatcg cgaactcgtc gcctcccagg cgcgagaaga tctcattgcg ccgcacctgt
     8101 cccgccacct cgcccgcgac gcggatcagc atcgcgtccc ccgcgcggtg gccgaaggtg
     8161 tcgttgatgt acttgaagtc gtcgaggtcg tagaacagca gcgccacccg tgaatcgtgg
     8221 cgctgggcgt ccgcgatcat gcgcgccagc tcctcgttga agcggtggcg gttgaagagg
     8281 ccggtgagcg cgtcgcgctc ggcgaggtag atcagctggt ccgcggtctg gcgctcgcgg
     8341 gtgacgtcct ggaacagcca gagatggccg accggccgcc cgtagacgtc ctccacggcg
     8401 tgcacctgct gggtgatcag gcggccgtcc gagatctgga tctccagcac gccgaggatc
     8461 tcgtcctcgc gcggcaggcg cagcacgtgc ttgccttgct cttccggccg cgccagcgcg
     8521 cacgccgagg aggcgagcgc ctcctgcggg ctcttgccga tcaaccgcgt gcccggcgcg
     8581 atcatccaga tgcgggtaaa cgcgggattc gtgtacacca cccggttgtc agaggacacg
     8641 aacaggatcc cggtgtccat cgccgcgagc agcgccgaga gccgcgcgtg ctcgtcctgc
     8701 gcgcgcgtga ggtattcctg ctgaatcgcc tgcgcgtcgc gcagctcgac caccgtcgcc
     8761 tcgagccgct gctccgcctc cttgcgctgg gtgatgtcgc ggatgctgat gcggatgccg
     8821 agatagcccc cgcggctgtc gtagatcgga cgccagtcgg ccgccgccca gaattcggag
     8881 ccgtccttgc gcttgccgcg gaattcgtaa tcctggccgg tcccgccgcg caatgcgcgg
     8941 cggatctgcc gcaccgtgcg cgtgacgtcg gcctcggcga tgaagggggc gggaaagttc
     9001 tgcgtggcga ggcattcctc cggcgtgtag ccgaacatgt cgagcacgcg cggattgatc
     9061 cagatgagcc tgccttcctg gttgatccac agctcgcagt cgtagctgta gtcggcgatc
     9121 gcggtgaact tggcctcgct ctccgcgagc gccttgatgc ggccctggag cgccgccgcc
     9181 atggcgttga aggcatgggt gagcgtgccc acctcgtcgc cgccttccac cggcagccgt
     9241 acgttgaggt cgcccgccgc caggctctcg ctcgctttgg tgagccgggt gagattgcgg
     9301 gtgagccagt aggaaagcgc gaccgtcgaa acgatggaca ggatcagggc gatgccgccg
     9361 atgacgaggt tttcgcggat caggtcggcg cgcgcggcgc gcatgaacgc ggtggacagg
     9421 ccgaaatgaa ggcttccgat cttgcgcccc gccgtctcga tgtccatcgc gctgtgaacg
     9481 tgctcccgct gtccgacgaa cggctttttc gcgggcgcct cgacgggcgg caacgggctg
     9541 ctctgctccc aacctgcgct cgccaccagc ttctcgctcc ggtcgaacag caccaggtag
     9601 atgatgccgt tgtcgcggcg cacgtgctcg aggatctcgg cgagcgcatc gcgctgcccc
     9661 gcggcgagcg ggaccgagag cgcggcgttg agcagctgct tctgttcctc gaggtggatg
     9721 ctcgtgcgct cgttgagctt gtcctccatg atgctgatgc cgttgacgat caggagcagg
     9781 agcatcatca cgttcaccgc cacgccgccc agcagcaggc gcgcgcgcaa cgacggcgag
     9841 agcggcactt tcgtcatggg acggctctca caatcgcttc accagcacca cagagcggcg
     9901 ctggtagttg taaagcaggc gtttctggct cggcagcagc gccacgccca cttcggcgaa
     9961 gccacgttcg acgaaccagt gctcggcgcg cgtcgtcagc acgaacagcc gcttcaactt
    10021 ctgccggcgc gcccgcccct cgatctcgtt cagcagccgc tcgccggcgc ccaggttccg
    10081 gaaatcggga tgcacaacga gacaggcgag ttcacccgcc cgttcgtcgg ggaacgggta
    10141 gagcgcggcg cagccgatca ccagcttgtc gtgctcgagc acgagaaagc gcccgatctc
    10201 ctgctcgagc cggccgcgcc cgcgcttgac gaggatgcct tcggcttcga gcggctcgat
    10261 cagcctcagt atgccgccga tgtcctcgat cttcgcctgc cgcagcgtct cgatcgggtc
    10321 gggcgtgacc atggtgccga cgcccttatg ggtgaagagc tcgagcagca gcccgtcgtc
    10381 gatgtgcccc gagatgaggt gcgcgcgtct caccccgtgc tcgca
//
LOCUS       DBNGWZ010000597         5053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_596545, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000597 DBNGWZ010000000
VERSION     DBNGWZ010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_596545"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..548
                     /gene="ybgC"
                     /locus_tag="AC16CX_18600"
     CDS             84..548
                     /gene="ybgC"
                     /locus_tag="AC16CX_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR3014552.1"
                     /translation="MVTPRDAAARKTTDASTFRWSVRVYYEDTDLGGVVYYANYLRFL
                     ERARTEWLRALGFGQTALAAEHRVVFVVRSISLDYLKPARFDDALEVTVEPAEIGASR
                     IVLSQRVRRGTEDLVAARVELACVNTATFKPVRIPSSLSDLLRRQAAESKTA"
     gene            731..1459
                     /gene="tolQ"
                     /locus_tag="AC16CX_18605"
     CDS             731..1459
                     /gene="tolQ"
                     /locus_tag="AC16CX_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR3014553.1"
                     /translation="MSPTVTHDMSVLSLVTGASVLVQIVMLMLVLASVFSWYYIFLKI
                     FTFRRANRLADRFEKEFWSGADLNELYQRAINARDSAGSQERIFEAGFREFAKLRRQP
                     GMDVAVVMDGVRRAMRATYQREMERLESYLSFLASVGSVSPYVGLFGTVWGIMNSFRS
                     LANVAQATLGAVAPGIAEALIATAMGLFAAIPAVVAYNRFAGDINRLATRFDSFMEEF
                     SNILQRQGPVKPVSSERSAVSSEK"
     gene            1529..1957
                     /gene="tolR"
                     /locus_tag="AC16CX_18610"
     CDS             1529..1957
                     /gene="tolR"
                     /locus_tag="AC16CX_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HIR3014554.1"
                     /translation="MLIDRRIGRRLMNQINVVPYIDVMLVLLVIFMITAPLINPGQIE
                     LPSVGKTSDPPVQPMEVSVRTDGTLWLRDRALSMEERKISRNELIAAIRQKQKRNPDQ
                     AVVIAADKDVRYEAVLEVMDMLQQNQVRKVGLLAKPRSSK"
     gene            1954..2721
                     /gene="tolA"
                     /locus_tag="AC16CX_18615"
     CDS             1954..2721
                     /gene="tolA"
                     /locus_tag="AC16CX_18615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HIR3014555.1"
                     /translation="MTAYVYETHNEKAVSGALTLVMHLLFFALLVFGVTWQKRETPAM
                     VAELWSNLPQPAPEAEPSPPPPKAEPEPPKPPPKAEVKPEPKVEPKPAPKADIELREK
                     KEKARKAKEREIAEKKKREELARIEALKQQQAKEAEAKRLAREQEAALRKLAQSQAAA
                     QAKEVDRYKRLIAERIKRFIIEPPNLQGNPEVELDVIVLPGGEVLDVRTRRASGQAPW
                     DNAVERAVRRAQPLPLPPDPALMKEFRELNLKFRPKE"
     gene            2914..4242
                     /gene="tolB"
                     /locus_tag="AC16CX_18620"
     CDS             2914..4242
                     /gene="tolB"
                     /locus_tag="AC16CX_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR3014556.1"
                     /translation="MIKEFAGPKSSGKFLDRLHCFFRAAAVFVLLVLSPAAHAVLTIE
                     IIGTGANQFPVAIVPFRAETGLPQPLAPVISADLARSGLFRMVDAGGLNPPPHEPQDL
                     NYGTWRARGAEAVVIGSVAPLPNGRYDVRFRLMDVAKQAQLAGFAYTASGEQLRHTAH
                     RIADVIYEKLTGDKGVFSTRITYVVKRGNRFELQVADADGFGAQTVLASNEPIISPAW
                     SPDGTQLAYVSFEQKKPVVYVQSLTTGSRRALANFWGSNSAPAWSPDGRRLAVVLTKD
                     GGSQIYLISTDGSSVTRLTQSSAIDTEPNFSPDGQLLLFTSDRGGSPQIYGMSASGGD
                     VRRLTFEGSYNVTPRFSPDGKSFTFIQRNGSRFNVAVQDLTTRQVQVLTDGAVDESPS
                     FAPNGRMILYATEVGGRGILSAVSSDGRVKQRLTADAGDVREPAWGPLPK"
     gene            4268..4819
                     /gene="pal"
                     /locus_tag="AC16CX_18625"
     CDS             4268..4819
                     /gene="pal"
                     /locus_tag="AC16CX_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR3014557.1"
                     /translation="MKRLILSVLMTAVLAACGTTPTKEQEGAAVEDRKPGVVVDRPPV
                     KPADPKAIAKPLVDKPLAGSPLKDPGNILSKRSIYFDYDSNLVKEEFKPVVAAHARYL
                     QQNGGTKMRIEGNADERGSREYNLALGQRRADAVKQMMQLLGAQALQIETVSFGEEKP
                     KCTGHEENCWWQNRRGDIVYQGE"
BASE COUNT          948 a         1584 c         1682 g          839 t
ORIGIN      
        1 ggtgagcggt gagcagtgag cggtgagccg taaggtaatt ttcccggggt tttccgctta
       61 ccgctgacgg cttaccgctt accttggtca caccacgcga cgctgccgca agaaagacta
      121 cggacgcaag cactttccgc tggtcagtaa gggtttatta cgaggacacc gacctcggtg
      181 gggtcgtcta ttacgccaat tacctgcgct ttctcgagcg ggcgcgcacc gagtggttgc
      241 gcgcgctcgg cttcgggcaa accgcgctcg ccgccgagca tcgggtggtg ttcgtggtgc
      301 gctcgatctc gctcgactat ctgaagcccg cgcgcttcga tgacgcgctc gaggtcaccg
      361 tcgagcccgc cgagatcggg gcgagccgca tcgtattgtc gcagcgcgtc aggcgcggca
      421 cggaggacct cgtcgccgcc cgcgtggagc tcgcctgcgt caacacggcc acgttcaagc
      481 ccgttagaat accgagcagc ctgtcggact tgctgcgccg acaggctgct gaaagcaaaa
      541 cagcctgagg actcgggatg aatttcaatg aaatgaccca ttctggcctg cgccggagac
      601 ggaagagagc tggtgttcgt tcctccaggt gcttcgtcgc ccgttttttt gcgggcggtt
      661 ttgctattag gagtttgtcg gaggaacggc cgacaaactc ctccgtcata gcaagaattg
      721 gaaatccatc atgagtccca ctgtcactca tgacatgtcg gtgttgagcc tcgtcaccgg
      781 cgcgagcgtg ctggtgcaga tcgtgatgct gatgctggtg ctcgcctccg tcttctcctg
      841 gtattacatc ttcctcaaga tcttcacctt ccggcgcgcg aaccggctcg ccgaccggtt
      901 cgagaaggag ttctggagcg gcgcggacct gaacgagctc taccagcgcg cgatcaatgc
      961 gcgcgattcc gcgggcagcc aggagcgcat cttcgaggcg ggcttccgcg aattcgcgaa
     1021 actcaggagg cagcccggca tggacgtcgc ggtcgtcatg gacggcgtgc ggcgcgccat
     1081 gcgcgcgacc taccagcgcg agatggagcg gctcgagtcc tacctctcgt tcctcgcctc
     1141 ggtggggtcg gtgagcccgt atgtcgggct gttcggcacg gtgtggggga tcatgaattc
     1201 gttccgcagc ctcgccaacg tcgcgcaggc gacgctcggc gcggtggcgc cgggcatcgc
     1261 ggaggcgctg atcgcgaccg ccatgggcct gttcgcggcg attcccgcgg tggtggccta
     1321 caaccgattc gccggcgaca ttaaccggct tgccacccgc ttcgactctt tcatggagga
     1381 attctccaac atcctgcagc gccagggccc ggtgaaaccg gtgagcagtg agcggtcagc
     1441 agtgagcagt gaaaagtaag gtgcgtgaaa atgtcttacg tccgagctta ttgatgattt
     1501 acagcttacc gcttacggga ctgaaatcat gctgatcgac aggcgtatcg gtcgccgtct
     1561 catgaaccag atcaacgtcg tgccgtacat cgacgtgatg ctggtgctgc tggtgatctt
     1621 catgatcacc gcgccgctga tcaaccccgg ccagatcgag ctgccgtcgg tcggcaagac
     1681 ttccgacccg ccggtgcagc cgatggaggt ttcggtgcgc accgatggca cgttgtggct
     1741 gcgcgaccgc gccctctcga tggaagagcg caagatctcg cgcaacgagt tgatcgccgc
     1801 gatccgccag aagcagaaac gcaaccccga ccaggcggtg gtgatcgcgg cggacaagga
     1861 cgtgcgttac gaggcggtgc tggaagtcat ggacatgctc cagcagaacc aggtgcgcaa
     1921 ggtgggcctg ctcgccaagc cgcgctcgag caaatgacgg cctacgtcta cgaaacgcac
     1981 aatgagaagg cggtctccgg cgcgctcacg ctggtgatgc atttgctttt cttcgcgctg
     2041 ctggtgtttg gcgtcacctg gcagaaacgc gagactcccg cgatggtggc cgagctgtgg
     2101 agcaacctgc cgcagccggc gcccgaggcc gagccttcgc cccctccgcc caaggccgag
     2161 cccgagccgc ccaaaccgcc gcccaaggcg gaggtgaaac cggagcccaa ggtcgagccc
     2221 aagcccgctc ccaaggccga catcgagttg cgcgagaaga aggaaaaagc gcgcaaggcg
     2281 aaggagcgtg agatcgccga gaagaagaag cgcgaggagc tggcgcggat cgaggcgctc
     2341 aagcagcaac aggccaagga ggcggaagcg aagcgcctcg cccgcgagca ggaagcggcg
     2401 ctgagaaagc tcgcccagag ccaggcggcg gcccaggcga aggaggtgga caggtacaag
     2461 cggctcatcg ccgagcggat caagcgcttc atcatcgagc cgcccaacct gcagggcaat
     2521 cccgaggtcg agctcgatgt catcgtgctg cccggcggcg aggtcctgga cgtgaggacc
     2581 cggcgcgcga gcgggcaggc gccctgggac aacgcggtcg agcgcgcggt ccgccgggcg
     2641 cagcccctgc cgctgccgcc cgatcccgcg ctcatgaagg aattccggga actcaatctc
     2701 aaattccggc ccaaagagta gagcgcatct cgagaattcc tctaccaaca gtcgggggag
     2761 agcgcatttt cgagatgcgc tctaccaaat gaattcatcg attcccgact tgtcgcctgc
     2821 cagcgacacg tggcaaacga cgttcgggag gatgcaaagc agttataatt atcggacatg
     2881 agcggtttgc gtagccccga tttgggggtg gttgtgatta aggaatttgc cggaccgaaa
     2941 tcatccggca aattcctcga caggctgcac tgcttcttcc gcgccgccgc cgtctttgtc
     3001 ctcctcgtgc tgtcgccggc ggcacacgcc gtcctcacca tcgagatcat cggcaccggc
     3061 gccaaccagt ttcccgtcgc catcgtgccg ttccgcgcgg aaacagggct gccgcaacct
     3121 ctcgcgcccg tcatttccgc cgatctcgcc cgcagcgggc tgttcaggat ggtggacgcc
     3181 gggggactca acccgccgcc gcacgagccg caggacctga actacggcac ctggcgcgcg
     3241 cgcggcgcgg aagccgtggt gatcgggagc gtcgcgccgc tgcccaacgg ccgctacgac
     3301 gtgcgcttcc gcctgatgga cgtggcgaag caggcgcagc tcgcgggttt cgcctacacc
     3361 gcttcgggcg agcagctgcg gcacaccgcg caccggatcg cggacgtcat ctacgaaaag
     3421 ctgaccggcg acaagggcgt gttctcgacc cgcatcacct acgtggtgaa gcgcggcaac
     3481 cgcttcgagc tgcaggtggc ggacgccgac ggcttcgggg cgcagaccgt gctcgcctcc
     3541 aatgagccga tcatctcgcc ggcgtggtcg ccggacggca cccagctcgc ctacgtctcg
     3601 ttcgagcaga agaagccggt cgtctacgtc cagtcgctca cgactggcag ccgccgggcg
     3661 ctggcgaatt tctggggcag caacagcgcg cccgcctggt cgcccgacgg caggcggctc
     3721 gccgtcgtgc tcaccaagga cggcggctcg cagatctatc tcatcagcac cgacggcagc
     3781 agcgtcaccc ggctcaccca gagctcggcg atcgacaccg agccgaattt ctcgccggac
     3841 gggcagttgc tgctgttcac gtccgaccgc ggcggcagcc cccagattta cgggatgtcg
     3901 gcgtcgggag gggacgtgcg gcggctgacg ttcgaaggca gctacaacgt caccccgcgc
     3961 ttcagccccg acggcaagag cttcacgttc atacagagga acggcagccg cttcaacgtc
     4021 gcggtgcagg acctgacgac gcggcaggtg caggtgctca ccgatggcgc cgtcgacgag
     4081 tcgccgagct tcgcgccgaa cggcaggatg atcctctacg caaccgaggt gggggggcgt
     4141 ggtatattgt ccgcggtctc gagcgatggc agggtgaagc agcggctcac cgcggatgcc
     4201 ggcgacgtgc gcgagccggc gtggggtcca ttaccgaagt gacaatcgag tgaagaggag
     4261 ccaggccatg aagagactga tattgagcgt attgatgacg gcggtgctcg ccgcttgcgg
     4321 caccaccccg accaaggagc aggaaggcgc cgcggtcgag gaccgcaagc cgggcgtggt
     4381 ggtggaccgg ccgccggtga agcccgcgga tcccaaagca atcgccaagc cgctggtgga
     4441 caagccgctg gcgggcagtc ccctgaagga cccaggcaac atcctctcca agcgcagcat
     4501 ctacttcgac tacgactcga acctcgtcaa ggaggaattc aagccggtcg tcgccgcgca
     4561 cgcaaggtat ctgcaacaga acggcggcac caagatgcgc atcgagggca acgccgacga
     4621 gcgcggcagc cgcgagtaca acctcgcgct cgggcagcgc cgggcggacg cggtgaagca
     4681 gatgatgcag ctactcgggg cgcaggcgct ccagatcgag accgtgagct tcggcgagga
     4741 gaagccgaag tgcaccgggc acgaggagaa ctgctggtgg cagaaccgcc gcggcgatat
     4801 cgtctatcaa ggcgagtgag cgtggaggga tgaggggtga gccgggaatt cgaagaacgt
     4861 gcctgcgcac gttcgagccg gcgaacgccc gacgcgatgc agcggggcgg gccggggtcg
     4921 tagaccaggg atgagggatg aaggtcaaac cctcggcttc gtccggtttt cttcgttgct
     4981 tcgcgtggat tgccgtcgta gcgggtctta atccggcggt gcacgtttcc gccggattgt
     5041 tcgacgacga ggt
//
LOCUS       DBNGWZ010000598         1703 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2233997, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000598 DBNGWZ010000000
VERSION     DBNGWZ010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2233997"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="AC16CX_18630"
     CDS             <1..957
                     /locus_tag="AC16CX_18630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3014558.1"
                     /translation="KLKQNVTLRTIEEMAQRCKNWGKWGPDDELGTLNYVTRKDIVRA
                     AALVKKGKAFSLGLDFNQNGPQRTGWGGRFNPIHTMLATGTDAVAGRQDAGKIRYADD
                     MVSMPLQCGTQWDALGHIFYGDKMWNGYDARLVDSTGAKKNGIENTRDRMIGRGVLLD
                     IPRWKGGDYLEDGYGVGNDDLDGCARAQGVQIRRGDFVIVRTGQMEHCLAQGDWGAYA
                     AGDAPGLRFETCEWIYKKQIAAICFDTWGCEVRPNESKEAFQPWHWVVIPMIGITMGE
                     IFYLKGLADDCRKDRVYEFLFCGPPLPITGAVGSPINPMAVK"
     gene            1129..>1703
                     /locus_tag="AC16CX_18635"
     CDS             1129..>1703
                     /locus_tag="AC16CX_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014559.1"
                     /translation="MTLSLPPERLPRIPPEKMTEAQKKAAAELAAGPRGELRGPFIAL
                     LRSPGLMSAVQKVGEYLRFNCPLERRVTEFATLIAARHWTQQYEWQAHYEHAMKAGLK
                     PALADAIGEGRRPAGMAEDEEIVYDLLTESLHNRSVSDATYARAVAKFGETGVVDLVG
                     LAGYYLMLAMIMNMARTALPAGKSPQLMPFP"
BASE COUNT          330 a          512 c          585 g          276 t
ORIGIN      
        1 aagctgaagc agaacgtcac cctgcgcacg atcgaggaga tggcgcagcg ctgcaagaac
       61 tggggaaaat gggggcccga cgacgagctc ggcacgctca actacgtgac gaggaaggac
      121 atcgtcaggg cggcggcgct ggtgaagaag ggaaaggctt tttcgctcgg gctcgacttc
      181 aaccagaacg gaccgcagcg cacgggctgg ggcggccgct tcaatccgat ccacacgatg
      241 ctcgcgaccg gcaccgacgc cgtcgcgggc cggcaggatg ccggcaaaat ccgttacgcc
      301 gacgacatgg tgtcgatgcc gctgcaatgc ggcacgcaat gggacgcgct cggtcacatc
      361 ttctacggcg acaagatgtg gaacggctac gacgcgcggc ttgtggattc caccggcgcc
      421 aagaagaacg gcatcgagaa tacgcgcgac cgcatgatcg gccgtggcgt gctgctcgac
      481 atcccgcgct ggaagggggg cgactacctc gaggacggct acggcgtcgg caatgacgac
      541 ctcgacggct gcgctagggc gcaaggcgtg cagatccggc gcggtgactt cgtgatcgtg
      601 cgcaccggtc agatggagca ctgcctcgcg cagggcgact ggggcgccta tgcggcgggg
      661 gatgcgccgg ggttgcgctt cgagacctgc gaatggattt acaagaagca gatcgccgcg
      721 atctgcttcg acacctgggg ctgcgaggtg cgcccgaacg aaagcaagga ggcgttccag
      781 ccgtggcact gggtggtgat tcccatgatc ggcattacta tgggggagat cttctatctc
      841 aagggtctcg ctgatgactg ccggaaggat cgggtctacg aattcctctt ctgcggcccg
      901 ccgctgccga tcaccggggc ggtggggtcg ccgatcaacc cgatggcggt caagtgaaac
      961 agtgacgagt gtcgagtgtc gggggtcgag tgtcgggggt caaggggaag gagccgttga
     1021 ctcattgttt tcactcgaca ctcattcctc gacaccccac tcagccccca tccctgatca
     1081 gttgcttcat caagcacggt aaatttattc atccagacgg agtttgaaat gacgctatcg
     1141 cttccacccg agcgcctgcc gcgcatcccg cccgagaaga tgaccgaggc gcagaagaag
     1201 gccgccgccg agctcgccgc gggcccgcgc ggggagctgc gcgggccgtt catcgcgctg
     1261 ctgaggagtc ccggcctgat gagcgcggtg cagaaggttg gcgaatatct acgcttcaat
     1321 tgcccgctcg agcggcgcgt cacggaattc gcgacgctga tcgcggcgcg gcactggacg
     1381 cagcagtacg aatggcaggc ccattacgag cacgcgatga aagcgggact caagcccgcg
     1441 ctcgcggacg cgatcggcga agggcggcgc cctgccggca tggccgagga cgaagagatc
     1501 gtctacgacc tgctgaccga gtccctgcac aacaggagcg tgagcgacgc gacctatgcc
     1561 cgggcggtcg cgaagttcgg cgagacgggg gtggtggatc tcgtcgggct tgccggctac
     1621 tacctgatgc tggcgatgat catgaacatg gcgcgcaccg cgctgccggc cggcaagtct
     1681 ccgcagctca tgccgtttcc cta
//
LOCUS       DBNGWZ010000599         1985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_596816, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000599 DBNGWZ010000000
VERSION     DBNGWZ010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_596816"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1517)
                     /locus_tag="AC16CX_18640"
     CDS             complement(276..1517)
                     /locus_tag="AC16CX_18640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3014560.1"
                     /translation="MYDLHLTPEQLEFRDTVRDFVENEVKPAALNPKRLEPFEKPLLT
                     GVLDNASQMGLRTLALSEAAGGAGADGLTACIVLEELAAGDVDVAVVLGQTATLAHTL
                     FDRLMTHAQRSRFLPKFVADERYHLAFAGRDPDAGLGWSYHRPLAEEGGEPTAVKQGN
                     EWVINGTIPFVANAPVAKLFAIQVRTDPKKGMNGVSTLLVPRDAPGLTVREPLKAVGE
                     TVRWHHGAGAGVVLKDCRVPADHLIGKEGQSPFASRAEHARGIPLLAAVNLGVARAAY
                     EAAVDYARIRRQGGRNIIEHQAIGTKLADCAIQLELARNVIWKAAWTLDHPEAVADRS
                     VSDLPLYVIARVYTAEAAHEVTLLAAECFGAMGVMRDMPLQKYVHDAMVFLHSEDTDG
                     AAKLAVAEAIAGYQRPAADAA"
     gene            complement(1710..>1985)
                     /locus_tag="AC16CX_18645"
     CDS             complement(1710..>1985)
                     /locus_tag="AC16CX_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HIR3014561.1"
                     /translation="YADDTCAVSATGPGEHFIRAVLAHDVATRMRYLGETLGQAARRA
                     LARVVALGGDGGLIAVDRAGNVAMPFVSEGMYRGVARGGRFTVAIYR"
BASE COUNT          318 a          687 c          636 g          344 t
ORIGIN      
        1 tcgatgatgt tccagtccca cggctcgaac ccgccctcgg tctggtcgcg ctgcatcgag
       61 atcgggcgga tctcgtcttc cgcgaacctg cgcgcctccg tctgccagcc gcgctgctct
      121 tcattcaacg aaaagtccat ttcctactcc tcaaaatacg ggagatcgtg aaatcgggag
      181 atcgtgagat ggattgtcgc tctcacgccc tcccgctctc acggattatg tcgctctcgc
      241 gctcttacgc tctcccgccc tcacgtccta agcggtcagg cggcatccgc cgccggccgc
      301 tggtagccgg cgatcgcctc ggctaccgcc agcttcgccg caccgtccgt gtcctcggaa
      361 tgcaggaaca ccatcgcgtc atgcacgtac ttctgcaacg gcatgtcgcg catcacgccc
      421 atggcgccga agcactccgc cgcgagcagc gtgacctcgt gggcggcttc ggcggtgtag
      481 acgcgcgcga taacgtaaag cggcaggtcg gacacgctgc ggtcggcgac cgcctcggga
      541 tgatccagcg tccacgccgc cttccagatc acgttgcgcg ccagctccag ctggatcgcg
      601 cagtcggcga gcttggtgcc gatcgcctgg tgctcgatga tgttgcgccc gccctggcgc
      661 cggatcctgg catagtccac cgcggcctcg taagcggcgc gcgcgacgcc gagattgacg
      721 gccgcgagca acgggatgcc gcgcgcatgt tccgccctgc ttgcgaacgg gctctgtcct
      781 tcttttccga tcagatgatc cgcgggaacc cggcaatcct tgagcaccac ccctgcgccc
      841 gcgccatggt gccagcggac cgtctcgccg accgccttca agggctcgcg cacggtcaga
      901 ccgggcgcgt cgcgcggcac gagcagcgtg ctcacgccgt tcatgccctt cttcgggtcg
      961 gtccggacct ggatcgcgaa gagtttcgca accggggcgt tcgcgacaaa cggtatcgtg
     1021 ccgttgatca cccattcgtt cccctgcttc actgcagtcg gttccccgcc ttcctcggcg
     1081 agcggccggt ggtagctcca cccgagaccg gcatcgggat cgcgtcccgc gaaggcgagg
     1141 tgatagcgct cgtccgcgac gaatttcggc agaaagcggc tgcgctgcgc gtgcgtcatc
     1201 agccggtcga acagggtgtg ggccagcgtc gcggtctgcc cgagcacgac cgcgacgtcc
     1261 acgtcgcccg cggcgagctc ttccaggacg atgcaggcgg tcaggccgtc ggcacccgcg
     1321 ccgcccgccg cttccgagag cgccagcgtg cgcaggccca tctgcgacgc attgtcgagc
     1381 acgcccgtga gcagcggctt ctcgaacggc tcgaggcgct tcgggttcag cgcggcgggt
     1441 ttgacttcgt tctcgacgaa atcccgcacg gtatcgcgga attccagttg ctctggggtc
     1501 aggtgaaggt catacatatc ggcgttccat actctggtcg gtcagtaacg caagtttaac
     1561 agccgccgga aaagcagtgc cagtatgtgg aatcgccgcc gaggaggttg gagtgtcgag
     1621 gaatgagtgc ctaggaaagc aacttgggac gaggattgag ggtcgagggc gagaaccgcg
     1681 gtcccccaac agtcgtcacc ccacactcgt cagcggtaga tggccacggt gaagcggcca
     1741 ccgcgcgcga cgccgcggta catgccttcg ctcacgaacg gcatcgccac gttgccggcg
     1801 cgatcgacgg cgatcagccc gccgtcgccc ccgagcgcga caacccgcgc gagagcacgc
     1861 cgcgcggctt gccccagcgt ctcgccgagg tagcgcatgc gcgtggcgac gtcatgggcg
     1921 aggactgcgc gaatgaagtg ctcgcccggt ccggtcgcgg agaccgcgca ggtgtcgtcg
     1981 gcata
//
LOCUS       DBNGWZ010000600         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2864231, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000600 DBNGWZ010000000
VERSION     DBNGWZ010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2864231"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16CX_18650"
     CDS             complement(<1..239)
                     /locus_tag="AC16CX_18650"
                     /inference="COORDINATES: protein motif:HMM:NF016532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 family protein"
                     /protein_id="HIR3014562.1"
                     /translation="MTLLDRILSMLPLTWLDAAALALFASLWAGYQWYSDYSATTRPR
                     LGREMDRFLREWIARMVERDNRMVDVNVLRNLTRSS"
     gene            complement(338..1444)
                     /locus_tag="AC16CX_18655"
     CDS             complement(338..1444)
                     /locus_tag="AC16CX_18655"
                     /inference="COORDINATES: protein motif:HMM:NF020084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR3014563.1"
                     /translation="MADFRYGHAAAKDWREAADACLAQLGQGPASLGFLYATDVIADH
                     LGDIVALFRSQTGVRHWVGTVGIGVCASGREYLDEPAIAAMVGDFEPDTFCVFSGVVN
                     AADVDNVALKCGGSTPNFAIVHVDPYNRDVAELVNKLAGKVESGFLVGGLTSSRRQNL
                     QIADGIMEGGLSGVSFSDSITIATRLTQGCSPIGPKHVVTACQHNVIISLDGRAALDV
                     FREDIGEALARDLNRIGGQIFAGLPITGSDTGDYLVRNLVGIDPANRLIAIGELLQQG
                     SNVMFCRRDTKTANEDMTRMLESIRKGLYSRPRGGIYYSCLGRGASLFGPNSEELKMI
                     REALGDFPLVGFLCNGEISHNRLYGYTGVLTLFV"
     gene            complement(1542..2132)
                     /locus_tag="AC16CX_18660"
     CDS             complement(1542..2132)
                     /locus_tag="AC16CX_18660"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3014564.1"
                     /translation="MKLYMTQNSGNAYKARLLLSMLKVPYEQAIVDFRSGEHKKEPFL
                     KLNPRGQVPVMEEEGRVFWGSTACLVYIARKHGGDRWLPTDPAGMAEVMQWLELAQNE
                     LHYGLQWARGVKTGIRKIGNYEEYQGYGNAGLAVLEGRLRNNQWLALGRPTIGDVACY
                     PYVSVSPEGGFKLEDHPGVLAWTKRFEALPGWVKRI"
     gene            complement(2348..>2563)
                     /locus_tag="AC16CX_18665"
     CDS             complement(2348..>2563)
                     /locus_tag="AC16CX_18665"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR3014565.1"
                     /translation="HWPVETRSARELVTNLFERYSTQSVSRAEALRQASLAVMQQTAQ
                     SADGRPLFSYAHPLFWAPYALVGDGGR"
BASE COUNT          445 a          847 c          821 g          450 t
ORIGIN      
        1 gagctgcgcg tgaggttgcg cagcacgttg acgtccacca tgcggttgtc gcgctcgacc
       61 atgcgcgcga tccactcgcg caaaaagcgg tccatctccc gtccgagccg cggccgcgtc
      121 gttgcgctgt aatccgaata ccactggtag ccggcccaca gtgacgcgaa cagcgccagc
      181 gcggcggcgt ccagccacgt gagcggcagc atggagagga tgcggtcgag cagcgtcatg
      241 tcggagcagc tgtcagcatt cagcgatcag ctttcagcct gaagcaagtg gcatgttttg
      301 gccgatagct gagagctgac cgctgagtgc tggttcatca tacgaacagc gtaagcacgc
      361 cggtgtagcc gtagagccgg ttgtgcgaga tctcgccgtt gcagagaaac ccgaccagcg
      421 gaaaatcgcc gagcgcctca cggatcatct tgagctcctc ggaattgggg ccgaacaggc
      481 tcgccccccg cccgaggcag gagtaataga tgccgccgcg gggacgcgag tacaggccct
      541 tgcggatgct ctccagcatc cgggtcatgt cctcgttcgc ggtcttcgtg tcgcgtcggc
      601 agaacatgac gttcgagccc tgctgcagca gctcgccgat ggcgatcagc cggttggcgg
      661 ggtcgatgcc gaccaggttg cgcaccaggt agtcgccggt atcgctgccc gtgatcggca
      721 gccccgcaaa aatttggccg ccgatgcggt tcaagtcacg ggcgagcgcc tcgccgatgt
      781 cctccctgaa cacgtcgagc gcggcgcgcc cgtccagact gatgatgacg ttgtgctggc
      841 aggcggtcac cacgtgcttg gggccgatcg gcgagcagcc ttgcgtgagg cgggtggcga
      901 tcgtgatgct gtcggaaaac gacacgcccg agagcccgcc ctccatgatg ccgtccgcga
      961 tctgcagatt ctgccggcgc gagctggtca gcccgcccac caggaagccg ctttccactt
     1021 tccccgccag cttgttcacc agctcggcaa cgtcccggtt gtagggatcg acgtggacga
     1081 tcgcgaaatt gggggtcgag ccgccgcact tgagtgcgac attgtcgaca tcggcggcgt
     1141 tgacgacgcc ggagaacacg cagaaggtgt ccggctcgaa gtcgccaacc atcgccgcga
     1201 tggccggttc gtccagatat tcccggccgc tggcgcaaac gccgatgccc accgtgccga
     1261 cccagtgccg cacgccggtt tggctcctga acaatgccac gatgtcgccg aggtggtcgg
     1321 cgatcacgtc ggtcgcgtag agaaatccga ggctggcggg cccctgcccc agttgcgcga
     1381 ggcaggcatc cgcggcttcc cgccaatcct tcgcggctgc gtgcccgtaa cggaaatccg
     1441 ccatggctca aaacattcga cgcaaaggcg caaagccgcg aagaagattt ggaattaatt
     1501 tcatcttgtc tcctttgcaa ctttgcggct tcgcgtcaaa ttcatatccg tttcacccag
     1561 ccggggagcg cttcgaaacg cttcgtccag gccagcacgc ccggatggtc ctcgagcttg
     1621 aagccgccct cgggcgacac cgacacgtag ggatagcagg cgacgtcgcc gattgtcggc
     1681 cggcccaacg cgagccactg gttgttcctc aggcggccct cgagcaccgc gaggccggcg
     1741 ttgccgtaac cctggtactc ctcgtagttg ccgatcttgc ggatcccggt cttcacgccg
     1801 cgcgcccatt gcaggccgta gtgcaactcg ttctgcgcga gctccagcca ttgcatcact
     1861 tccgccattc cggccggatc ggtcggcagc cacctgtcgc cgccgtgctt gcgggcgatg
     1921 taaaccaggc aggcggtcga gccccagaac acgcggccct cttcctccat caccggcacc
     1981 tggccgcgcg ggttgagctt caggaacggc tccttcttgt gctcgccgct gcggaaatcg
     2041 acgatcgcct gctcgtacgg caccttgagc atggaaagca gcaggcgcgc cttgtaggca
     2101 ttgccggaat tctgggtcat gtacagcttc atctttcctc cgtgagcgga cgggagttca
     2161 tcgtaacatg ccggccaagc ctgcaaacgg gccgcgccgg ccggcgtggc ccgggcgacg
     2221 aagcacactg gaatcgcgga cagacaagtg tgcggccggg aacgcgatga gccggaccgc
     2281 gagggccgcg gcaggtttgg tttcgcgcgc tcaagcgggt ggcgcccgct tcacggccga
     2341 tggcgggcta tcttccccca tccccgacga gcgcgtacgg cgcccagaac agcgggtggg
     2401 cgtacgagaa gagcggcctg ccgtccgcgc tctgcgccgt ttgctgcatc accgccaggc
     2461 ttgcctgtcg caaagcttcc gcgcgcgaga ccgactgagt ggaatagcgc tcgaacaggt
     2521 tggtcaccag ctcgcgcgcc gagcgcgtct ccaccggcca gtg
//
LOCUS       DBNGWZ010000601         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1604831, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000601 DBNGWZ010000000
VERSION     DBNGWZ010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1604831"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..645)
                     /gene="rfbC"
                     /locus_tag="AC16CX_18670"
     CDS             complement(103..645)
                     /gene="rfbC"
                     /locus_tag="AC16CX_18670"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR3014566.1"
                     /translation="MNVIPSTLPDVLILEPKVFSDERGFFFESYNARVLAQLAGITAS
                     FVQDNYSRSVKNALRGLHYQVQQPQGKLIRVTLGEIFDVAVDIRRSSPTFGQWTAATL
                     SDENKRMLWVPPGFAHGFLVVSDTADVLYKTTEFWAPQYERCIVWNDPDLGIPWPLDG
                     EPILSRKDRLGSLLRTAETF"
     gene            complement(649..>1512)
                     /gene="rfbA"
                     /locus_tag="AC16CX_18675"
     CDS             complement(649..>1512)
                     /gene="rfbA"
                     /locus_tag="AC16CX_18675"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR3014567.1"
                     /translation="GIILAGGSGTRLYPATQVVTKQLLPVYDKPMIYYPLTTLMFAGV
                     RDMLVISTPQDTPRYQQLLGDGGRWGIQLSYAVQPEPNGIPQAFLIGADFIGKAPCAL
                     ILGDNIFYGHDLSNDLKTATARTRGATIFAYPVHDPERYGVIEFDKNRKVISIEEKPK
                     RPKSRYAQTGFAVYDDRVVDIARALKPSARGELEITDVNLEYLRRGELEVVLMGRGTA
                     WLDTGTHDALLAASMFIETIEKRQGLKVACPEEIAYRLGYITAAALEALAASMGDSPY
                     ARYLREVLREL"
BASE COUNT          263 a          473 c          503 g          273 t
ORIGIN      
        1 ctcggcgcgg ctggtggcga cgagccggcc caatggcgcc agcgctgtca cgagctcgtg
       61 cccgacctgg ccgtccctac ccgtcagaag tatcgtgccc attcaaaacg tttcggccgt
      121 ccgcagcagg ctgccgagcc ggtccttgcg cgagagtatc ggctcgccgt cgagcggcca
      181 cggaatgccg aggtccggat cgttccagac gatgcagcgc tcgtattgcg gcgcccagaa
      241 ctccgtggtc ttgtagagca catcggcggt atcggagacc acgagaaacc cgtgggcgaa
      301 acctggcgga acccagagca tccgcttgtt ttcgtccgag agcgtcgctg ccgtccattg
      361 tccgaacgtc ggggagctgc ggcggatgtc cacggccacg tcgaaaatct cgcccaacgt
      421 cacccggatg agcttgccct gcggctgctg gacctgatag tggaggccgc gcagcgcatt
      481 tttgacggag cgagagtagt tgtcctgcac gaagctcgca gtgatacccg cgagctgcgc
      541 gagcacgcga gcgttgtagc tctcaaaaaa gaagccccgt tcatcgctga agaccttggg
      601 ctccaggatc aagacgtccg gcagggtgga cgggatgacg ttcatttttt acagctcccg
      661 caggacttcc ctgagataac gcgcgtacgg gctgtcaccc atggatgcgg cgagcgcttc
      721 gagggcggcc gcagtgatgt agcccaggcg gtaggcgatc tcctccgggc aggcgacctt
      781 caggccctgg cgcttctcga tggtctcgat gaacatcgag gcggcaagca gcgcgtcgtg
      841 ggtgccggtg tcgagccagg cggtgccgcg gcccatcagc accacctcga gctcgccgcg
      901 gcgcaggtac tcgaggttga cgtcggtgat ctcaagctcg ccgcgcgccg agggcttcag
      961 cgcccgcgcg atgtccacca ccctgtcgtc gtacacggcg aaaccggtct gggcataacg
     1021 cgacttgggc cgtttcggct tctcctcgat cgagatcacc ttgcgattct tgtcgaactc
     1081 gatcaccccg taacgctccg ggtcgtgcac cgggtaggcg aagatggtcg cgccgcgggt
     1141 ccgcgcagtc gcggtcttga gatcattcga aagatcgtgg ccgtagaaga tgttgtcgcc
     1201 gaggatgagc gcgcacggcg cctttccgat gaaatccgcg ccgatgagga aggcctgcgg
     1261 tatgccgttg ggctcgggct gcacggcgta cgagagttga atgccccagc gcccgccgtc
     1321 tccgagcaac tgctgatagc gcggcgtatc ctgcggcgtg gagatcacca gcatgtcgcg
     1381 gacgcccgcg aacatcagcg tagtgagggg atagtagatc atcggcttgt cgtacaccgg
     1441 cagcagctgc ttggtgacga cctgggtcgc cggatacagg cgcgtgccgc tgccgccggc
     1501 gaggatgatt cc
//
LOCUS       DBNGWZ010000602         3747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1854290, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000602 DBNGWZ010000000
VERSION     DBNGWZ010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1854290"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1677
                     /gene="hemN"
                     /locus_tag="AC16CX_18680"
     CDS             289..1677
                     /gene="hemN"
                     /locus_tag="AC16CX_18680"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HIR3014568.1"
                     /translation="MNSQTIEFDADLVRRLDRNGPRYTSYPTADRFVEAFGEEAYRAW
                     AMRRNIGGIQRPLALYVHLPFCRNICYYCACNKIVTRDASKAARYLEYLGREIDLQAA
                     LFRDDPRVAQMHWGGGTPTYYDTKDLRALFGRLTGHFEFAPHGEYSIEVDPRTVDAAM
                     IHALRELGFNRVSFGVQDFDPNVQVAVHRLQSEEQTRTVIEAARQAGFESVNVDLIYG
                     LPKQTLASFNNTLVHILALRPDRIALYNYAHLPTLFKSQRRIQEGDLPSPETKLKLLG
                     SAIKRFGVAGYVYIGMDHFALPQDALAVAQRQGRLYRNFQGYSTSAEADLVGLGVSAI
                     GAVGPTYSQNYRALEDYYDALERGKLPIMRGLELTADDLVRRAVIQALMCHFALSKES
                     LEIAYLIDFDSYFSTEIEELREFERLGLIAMKEGWLTVSPKGRLLIRNICMVFDRYLR
                     HDREVRRYSRVI"
     gene            1773..2546
                     /locus_tag="AC16CX_18685"
     CDS             1773..2546
                     /locus_tag="AC16CX_18685"
                     /inference="COORDINATES: protein motif:HMM:NF024778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3014569.1"
                     /translation="MSGWLEMVLASAFLAGLAGGAHCAAMCGGLAGIACGQRAGEIDR
                     AAWWGRALAYNAGRIASYVAAGVLAGGLGAAGVSLRGEPLTQQALLAVMSLALILFAV
                     YLTGFAPFVRPVEAAGGALWRHIQPHSRRFLPADSAGRAFGLGLLWGWLPCGMVYAAL
                     IAALATADPLYGGLVMAAFGAGTAPNLLAISAWFGYVVKVARARAARLLCAAVIAGVG
                     IFGFIEATQPAIHAGDGLCVTLPGIASLAGWNQALFAAR"
     gene            complement(2606..3127)
                     /locus_tag="AC16CX_18690"
     CDS             complement(2606..3127)
                     /locus_tag="AC16CX_18690"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR3014570.1"
                     /translation="MKKRVLTVACGALLVAGCAGMEEQREASAVAQLQPRSGSNVSGN
                     VRFTQIGDVVRVTGEITGHTKGPKGFHIHEKGDCSDDKAMSTGGHFNPHKSKHGGPYD
                     PVKHAGDLGNLVFNDQGAAKINFTVGDISVSRDRPDGIIGRAVIVHADRDDLNTDPTG
                     NAGGRVACGVITG"
     gene            complement(3320..>3747)
                     /locus_tag="AC16CX_18695"
     CDS             complement(3320..>3747)
                     /locus_tag="AC16CX_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3014571.1"
                     /translation="GGVGPNRAIVGYSAICAHRMSYPTPQISFISYREKSTASAIMRP
                     NTIHCCSEHSEYDPAAGAKVVSGPALQPLAAILLEHDAKNDTLHAIGTLGGELFNAFF
                     AKYEFKLAIDYGADNTRRQVAGLTEVKDLQQFCKQQVRC"
BASE COUNT          611 a         1218 c         1207 g          711 t
ORIGIN      
        1 agaggtgctc gcctttacgg atccggcgcc gctgattgac gagccggtcg attttctcca
       61 tttcccgcgc ctcgagcccc gccggcaggc acagttcccg caggctgcac gaggagcagg
      121 cgacgcgtag ccggccgaaa tccacagtgc ggtgagcagg aggggttctc acagggggga
      181 gtgtgccgcg cccgggtcct tccggtttga cctatatcaa gggccgccgg gcccgctgtg
      241 caatactgag gcccgacccg gacggtgagc cgcacagcga cgccagccat gaattcccag
      301 acgatcgagt tcgacgccga cctggtgcgg cgcttggacc gcaacgggcc gcgttacact
      361 tcctatccga ccgccgaccg cttcgtcgag gcgttcgggg aagaagcgta ccgggcgtgg
      421 gcgatgcgcc gcaacatcgg cggcatccag cgcccgctcg cgctctatgt gcacctgccg
      481 ttctgccgca acatctgcta ctactgcgcg tgcaacaaga tcgtcacgcg cgacgcgtcc
      541 aaagcggcgc gctatctcga atatctcgga cgggagatcg accttcaggc ggccttgttc
      601 cgggacgacc cgcgcgtcgc gcagatgcac tggggcggcg gcacgccgac ctactatgac
      661 acgaaggacc tgagagcgct gttcgggcgg ctcaccggcc atttcgaatt cgcccctcac
      721 ggcgagtact cgatcgaagt ggatccacgc accgtggacg cggcaatgat ccacgcgctg
      781 cgcgaactcg gtttcaaccg cgtgagcttc ggcgtgcagg acttcgatcc caatgtgcag
      841 gtggctgtac accgcctcca gagcgaggaa cagacccgca ccgtgatcga agccgcgcga
      901 caggccggtt tcgagtcggt caacgtggac ctcatctacg ggctcccgaa acaaacgctg
      961 gcgagcttca acaacacgct cgtgcatatc ctcgccttgc ggcccgatcg catcgcactc
     1021 tacaactacg cgcacctccc gaccctgttc aagtcgcagc ggcgcatcca ggaaggcgac
     1081 ctgccgtcgc ccgaaaccaa gctgaagctc ctcgggtcgg cgatcaagcg gttcggcgtg
     1141 gcgggctacg tttacatcgg catggaccat ttcgcattgc cgcaggatgc gctggcggtg
     1201 gcgcagcgcc agggacgact gtaccgtaac ttccagggat actccaccag cgccgaggcc
     1261 gacctcgtcg ggctcggcgt ctccgcgatc ggcgccgtcg ggccgaccta cagccagaat
     1321 taccgcgcgc tcgaggacta ttacgacgcg ctggaacggg gaaaacttcc gatcatgcgc
     1381 ggcctcgagc tcaccgccga cgatctcgtg cgccgggcgg tgatccaggc gctgatgtgc
     1441 cacttcgcgc tttcgaagga gtcgctcgag atcgcctatc tgatcgattt cgacagctac
     1501 ttctcgaccg agatcgagga gctgcgcgag ttcgagaggc tggggctcat cgccatgaag
     1561 gaaggctggc tcacggtgag cccgaagggg cggctgctga tccgcaacat ctgcatggtg
     1621 ttcgaccgct acctgcggca cgatcgcgaa gtgcgccgct attccagagt catatgacca
     1681 cgtgagagcg taagagcgtg agggcgcgag atcgaaaccg cgccttttat ctcccgacct
     1741 cccgatttca cgatctcccg ttatttcgaa gaatgagcgg ctggcttgaa atggtgctcg
     1801 cctcggcgtt ccttgccggt ctggcgggcg gcgcgcactg tgccgcgatg tgcggcgggc
     1861 tcgccggcat cgcctgcggc cagcgagctg gcgaaatcga tcgcgccgcg tggtggggaa
     1921 gagcgctcgc ctacaacgcc ggccgtatcg cgagctatgt ggctgcgggc gtgctggcgg
     1981 ggggtctcgg tgcggcagga gtgtccctgc gcggcgaacc gctgacacag caggcgctgc
     2041 ttgccgtgat gagcctcgcg ctcatcctgt ttgccgtcta cttaaccggc ttcgcgccgt
     2101 ttgtgcgccc cgtcgaggct gcgggcggag cattgtggcg ccacatccag ccgcactcgc
     2161 gccggttcct gccagccgat tcagcagggc gcgcgttcgg gctcggactg ctgtggggct
     2221 ggctgccgtg cggcatggtc tatgctgcgc tgatcgccgc gcttgccacc gcggatccgc
     2281 tgtatggtgg cctggtgatg gccgctttcg gcgcgggcac ggctcccaac ctgctcgcga
     2341 tcagcgcgtg gttcggttac gtcgtgaaag tcgcacgggc ccgggccgcg cgcctgctct
     2401 gcgccgcggt gatcgcgggc gtcgggatct tcggttttat cgaagcgaca cagcccgcca
     2461 tccacgcggg cgacgggctg tgcgtcacgc ttcccggaat cgccagcctc gccggctgga
     2521 accaagccct ctttgccgcg cgttgaacgg cgatcgattc gtccgttcga acgtgcggag
     2581 ctaaaccgga gccatgcttc cggatttagc ctgtaatcac gccgcaggcg acgcgcccgc
     2641 cggcgttgcc cgtgggatcg gtgttgaggt cgtcacggtc ggcgtggacg atcacggcgc
     2701 ggccgatgat gccgtcgggc cggtcccggc tcacggaaat gtcccccacc gtgaagttga
     2761 tcttcgcggc gccctggtcg ttgaagacga gattgccgag atcgcctgcg tgcttcaccg
     2821 ggtcgtaggg gccgccgtgc ttgctcttgt gggggttgaa gtgcccgccg gtgctcatgg
     2881 ccttatcgtc gctgcagtcg cccttctcgt ggatgtggaa gcctttcggg cccttggtgt
     2941 ggcctgtgat ctcgccggtc accctgacca cgtcgccgat ctgggtgaat ctcacgtttc
     3001 cactcacgtt cgagccgctg cgcggctgca actgggcgac ggccgacgct tccctctgtt
     3061 cttccatccc ggcgcaaccc gcgaccagca atgcgccgca tgcaacggtc agtacccgct
     3121 tcttcatata ccccctcctg aattaaccaa tgaaatgtgc gccagcaata ctatcctgca
     3181 ttcgggaggt acgggcaacg cgcgcctccc gggatggtga ctaagtggct gagtgatcaa
     3241 gtgattaagt caaagctact caatcactta gttactcaat cgcctaatca catgatgccc
     3301 gtcgcgcggc gacgggcatt cagcagcgga cttgctgctt gcagaactgc tgcaagtcct
     3361 tgacctccgt gaggcccgcg acttgccgcc gtgtattgtc ggctccgtag tcaatcgcca
     3421 gcttgaactc gtatttggcg aagaaggcgt tgaacagctc gccgcccagg gtaccgatcg
     3481 cgtgcagggt gtcgtttttc gcgtcgtgtt ccagcaggat cgccgcgagc ggctgcagcg
     3541 cgggtccgct cacgaccttc gcgcccgcgg ccggatcgta ctcgctgtgc tcggagcagc
     3601 aatgaatcgt gttgggccgc atgatcgccg aggcggtgga cttctcgcga tagctgatga
     3661 aactgatttg cggcgtggga tagctcatgc ggtgcgcgca aatcgccgaa tagccgacga
     3721 tcgcccggtt ggggccgacc ccgcccg
//
LOCUS       DBNGWZ010000603         6190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3366797, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000603 DBNGWZ010000000
VERSION     DBNGWZ010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3366797"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..957
                     /gene="rdgB"
                     /locus_tag="AC16CX_18700"
     CDS             343..957
                     /gene="rdgB"
                     /locus_tag="AC16CX_18700"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR3014572.1"
                     /translation="MRRLVLASNNPGKLREFRQMLAPLGIEVVPQSELGVTEADEPHG
                     TFIENALAKARHAASRSGLPAFADDSGICVSALGGAPGVQSARYAGDPPAGGDDQDER
                     NNRKLIAALEDKTDRRAHYYCVIVLVRHAGDPEPLIAEGRWHGEVVAAPRGAGGFGYD
                     PHFLVPELGRTAAELMPDEKNALSHRGRALAQLVVQLRVMGYAK"
     gene            947..2212
                     /gene="hemW"
                     /locus_tag="AC16CX_18705"
     CDS             947..2212
                     /gene="hemW"
                     /locus_tag="AC16CX_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR3014573.1"
                     /translation="MRNNQREVAVGINSSPIARRSSLHFRALPPLSLYVHIPWCARKC
                     PYCDFNSHEARAVIPEQRYVAALVADLEQALPQVWGRRVTSVFFGGGTPSLFSGRAID
                     TILAAVRARLTVEPDAEITLEANPGTVEAGKFRDFRAAGVSRLSLGIQSFNPRHLKAL
                     GRIHDDVEARRAIEIAKRNFDNVNLDLMYGLPGQTVEDALADIEEALAFDPPHLSCYH
                     LTIEPNTYFHRFPPELPGDDATAAMQEAIEARLAAAGYDHYETSAFAKPVTRHSSLVT
                     SRCAHNLNYWTFGDYLGIGAGAHGKLSFPERVVRQARVKHPERYMEGAERGEPVQEQR
                     EVHPREIPFEFMMNALRLNAGFPVALFEERAGLPLAAVLRELDEAERRGLIERDHRRI
                     APTALGRRFLNDLLQLFLAPEKQVPHPVS"
     gene            complement(2168..2893)
                     /gene="trmB"
                     /locus_tag="AC16CX_18710"
     CDS             complement(2168..2893)
                     /gene="trmB"
                     /locus_tag="AC16CX_18710"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR3014574.1"
                     /translation="MRVFPATTPGRPPFMPMITTRSPIRSYVLRQGRLTDAQRRACDE
                     LLPRYGIPFAREPLDLARVFGRAAPRILEIGFGMGETTARIAAGHPQNDYLGIEVHMP
                     GVGSLLKRVAELELANVRVIRHDAVEVIEHMIPPASLDGVHVFFPDPWPKKRHHKRRL
                     IQPPFVALLVSRMKRGAYLHVATDWEHYAQQIMEVLSAEPLLANTTDGFAPRPAWRPL
                     TKFENRGLRLGHRVWDLLFRRKE"
     gene            3063..>6190
                     /locus_tag="AC16CX_18715"
     CDS             3063..>6190
                     /locus_tag="AC16CX_18715"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3014575.1"
                     /translation="MEAAGRGNLMGSIYRRVSQTWPAYLLLVLGLAISFVAWRFTEQR
                     VIAEAATEFQQKTARAVETIDRRIQDNINLLLGLKGLFGASEHVSREEYQLYLSGFQL
                     ERRYSVIRVLSFSRYVKHRERAAFEQQVRRDTSVDPRGYPGFAIKPPGSRDEYVVIEY
                     FEPVAGNEAAFGLDLAAEPVRRAEIALARDSGEPVASGPLAVSINPNHISFSVRVAVY
                     RRGMPAQTVEQRRAAFHGVVAAVIHADELMASLFGTQLSNEFELVVHDVGPWQAARSE
                     APSKRNLLYDSRWLGGVEAESAGGPFRQVAMIDVAGRKWRLSFSLPAGGAGVERSLPI
                     VVFLGGIVTSLLLFWVVWTLLVSRERALKLAEHARAARAAEGLREQLAFIQQLIETVP
                     QPIFFKDAEQRRYLGVNKAWENFFGIPREQFIGKTVFELYPHNQELAQRHHAKDEELF
                     SRPGSQSYEAAIVAADGRVHHTIYNKATFNKSDGSVAGLIGTITDVTGLKEAEAALRE
                     SEARFRDLTELSSDWYWEQDAELRFTQISSKGREVSLGAEEDIGKRRWEVARLDMTEE
                     QWVPHKAGLAARQSFHDFTYQRHDTNGELRIISVSGRPIFDERGRFKGYRGTGRDITE
                     QKRAEERIRHMAHHDALTGLPNRALLHDRIGQAIVQAERNPRVFALLFIDLDRFKTVN
                     DSLGHQVGDRLLKTVAERLVACTRGSDTIARIGGDEFVVLLDDLNQPEDARHVAQKVL
                     ETLSEPVMLDTHELKVTPSVGICAYPHDGKDVETLMRNADIAMYHAKQMGRNNYQFFT
                     QAMNDAAQQRLQLENDLRHAVARGEFTLHYQPQLDLGTGAIIGFEALVRWLHPSRGLI
                     APSQFIPAAEETGLIAAIGEWVLREACSRSRAWHEAGHPQLQVSVNCSAQQFQREGFV
                     ETVGRILGETGLPAERLDLEITESVIIQRSEEVIARFQALDEMGARISIDDFGTGYSS
                     LSYLKRFAIHRLKIDQSFVRDISSDPDDAAIVSAIIAIAHSLGLEVVAEGVETSEQLA
                     FLKSLGCDAAQG"
BASE COUNT         1101 a         1989 c         2084 g         1016 t
ORIGIN      
        1 atgctcgacc tcgcgcagcg gggcatccgc gagctcgtcg ccgcgcagcg cgccgctctg
       61 caaaaataac cgctgagaag agcaatgcaa taattgaacc gccaagacgc caagagaaac
      121 aagacaacaa ttgaaccgca gagacgcgga gagaagcaaa gacagaattc actaacaggg
      181 aactgaattc ttgtttccgt acctgctctt ggttctctct ctgtctctgt ggttcgattg
      241 gtcttgattc tcggttccat tcaggtattt gcttctcttg gcgtcttggc ggttcgattg
      301 gttctttggg tcttatcatc attgcatctt ggcggttgat atatgcgtag actggtttta
      361 gccagcaaca accccgggaa gctgcgcgag ttccggcaga tgctcgcccc gctcggcatc
      421 gaggtcgtgc cccagtccga gctcggcgtg acggaagccg acgaaccgca cggcaccttc
      481 atcgagaacg cgctcgccaa ggcgcggcac gcagccagcc gctccggact gccggcgttc
      541 gcggacgatt ccggaatctg cgtgagcgcg ctcgggggcg cgccgggcgt gcagtcggcg
      601 cgctacgccg gcgatccgcc cgccggaggc gacgaccagg acgagcgcaa caaccggaag
      661 ctcatcgcgg cgctcgagga caagaccgac cgccgcgcgc attactactg cgtgatcgtg
      721 ctggttcgcc acgccggaga ccccgagccg ctgatcgccg agggccgctg gcatggagag
      781 gtcgttgcgg ccccgcgcgg cgccggtggg ttcggctacg atccccattt cctggtcccc
      841 gagcttggcc gtaccgcggc ggagcttatg cccgacgaga agaacgcgct cagccaccgc
      901 ggacgcgcac ttgcgcagct cgttgtgcaa ttgcgcgtaa tgggatatgc gaaataacca
      961 gcgagaagta gccgtgggca tcaactcatc acccatcgct cgtcgttcgt cattacattt
     1021 tcgcgcgctg ccgccgctgt ctttatatgt ccacattccc tggtgcgcgc gaaaatgccc
     1081 gtactgcgat ttcaactcgc acgaagcgcg cgcggtgatt ccggaacagc gttacgtggc
     1141 cgcgctcgtc gcggacctcg agcaggcgct gccgcaagtc tgggggcggc gggtgacgag
     1201 cgtattcttc ggcggcggca cgccgagcct gttctccggg cgggcgatcg acacgatcct
     1261 cgccgcggta agagcgcggc tcacggtcga gcccgatgcc gagatcaccc tcgaagccaa
     1321 ccccggcacc gtggaagcgg ggaagttccg cgacttccgc gcggcggggg tgagccggct
     1381 ctcgctcggg atccagagct tcaacccgcg ccacctcaag gcgctgggac gcatacacga
     1441 cgacgtcgag gcgcggcgcg cgatcgaaat cgcgaagcgc aatttcgata atgtaaacct
     1501 cgatctcatg tatggactgc cggggcagac cgttgaggac gcgttggccg acatcgagga
     1561 ggcgctcgcg ttcgacccgc cgcacctgtc gtgctaccac ctgaccatcg agcccaacac
     1621 gtacttccac cgctttccgc ccgagctgcc tggcgatgat gcgacggcgg cgatgcagga
     1681 ggcgatcgaa gcgagattgg ccgctgcggg ctacgaccac tacgagactt ccgccttcgc
     1741 gaagcccgtc actcgacact cgtcactcgt cacctcgcgg tgcgcgcata acctcaacta
     1801 ctggaccttc ggcgactacc tcggcatcgg cgccggcgcg cacggcaagc tttcgttccc
     1861 ggagcgcgtg gtgcgccagg cgcgggtgaa gcatcccgag cgctacatgg agggcgcgga
     1921 gcggggcgag ccggtccagg aacagcgcga agtgcatccg cgggaaatcc ctttcgagtt
     1981 catgatgaac gccctgcggc tcaacgcggg tttcccggtg gcattgtttg aagagcgggc
     2041 gggcctgccg cttgccgcgg tcctgcgcga gctggacgag gccgagcgcc gcgggctgat
     2101 cgagcgcgac caccggcgca tcgcgccgac cgcgctcggg cggaggttcc tgaacgacct
     2161 gctccagcta ttccttgcgc cggaaaagca ggtcccacac ccggtgtcct agccgcaggc
     2221 cgcggttctc gaacttggtc agcggccgcc acgccgggcg cggtgcaaac ccgtccgtgg
     2281 tattcgcgag cagcggctcg gcggaaagaa cctccattat ttgttgcgcg tagtgttccc
     2341 aatcggtcgc cacatgcaag taagcaccgc gcttcatccg tgacaccagc agcgcaacaa
     2401 atggcggctg gatcagccgc cgcttgtggt gccgcttctt gggccacggg tcggggaaga
     2461 agacgtgaac gccgtcgagg ctcgcgggcg gaatcatgtg ctcgatgact tccaccgcgt
     2521 catgccggat gacgcgcacg ttggcaagct ccagctcggc gacgcgcttg agcaggctcc
     2581 ccacgcccgg catgtggacc tcgatgccga gatagtcgtt ctggggatgg cccgcggcga
     2641 tccgggcggt ggtttcgccc atgccgaagc cgatttcgag gattctgggc gcggcgcgcc
     2701 cgaagacgcg cgcgagatcg agcggctcgc gcgcgaaggg gatgccgtaa cggggcagca
     2761 gttcgtcgca agcccgccgc tgcgcgtcgg tgaggcgtcc ctgccgcagc acgtagctgc
     2821 ggatcggact gcgggtcgtg atcatgggca tgaaaggagg acggcccggc gtcgtggctg
     2881 gaaaaacacg gattgcaccg cggcggattt tcgcagattc gcggcagggt agacagcccc
     2941 cgcctttccg cgcatcgcgc aatttccgcc gccggcggcg ccggagccgg cgaactgcgg
     3001 tttgaagccg ttccgcaaac agggtaacgt tagcgaaaag aaatccctcg ggggagggcc
     3061 tgatggaggc tgccgggcgc ggcaacctga tggggagcat ctacaggcga gtcagccaga
     3121 cctggccggc ttatctattg ctggtcctgg ggctcgcgat ctcgttcgtc gcatggcgct
     3181 ttaccgagca gcgggtcatc gccgaggccg ccaccgagtt ccagcagaaa actgcgcgtg
     3241 cggtcgagac catcgatcgc cgcatccagg acaacatcaa cctgttgctc gggttgaagg
     3301 ggctgttcgg cgcttccgag cacgtcagcc gggaggagta ccagctctac ctctccggct
     3361 tccagctgga acggcgctat tcggtcatcc gcgtcctttc tttcagccgg tacgtgaagc
     3421 accgtgagcg tgccgccttc gaacagcagg tgcggcgcga caccagcgtt gacccgcgtg
     3481 gttatccagg cttcgccatc aagccgccgg gcagccgcga cgagtacgtc gtcatcgaat
     3541 atttcgaacc ggtagccggc aacgaggcgg cattcggtct cgatctcgcg gcggaaccgg
     3601 tgcgtcgcgc cgaaatcgcg ctcgcccgcg acagcggcga gccggtggcc tccggccccc
     3661 tagcggtgtc gatcaatccc aaccacatat cgttctcggt gcgcgtggcg gtctaccgcc
     3721 gcggcatgcc ggcacagacg gtggagcagc gccgcgctgc tttccatggg gtggtcgcgg
     3781 cggtcatcca cgcggacgaa ctgatggcga gcctgttcgg cacgcagttg agcaacgagt
     3841 tcgagctggt ggtgcacgac gtggggccgt ggcaggcggc ccgatcggaa gcgccctcca
     3901 agcggaacct gctttatgac agccgctggc tcggcggcgt cgaggcggaa agcgcaggcg
     3961 ggccgttccg gcaggtggcc atgatcgacg tcgctggccg caagtggcgg ctgtccttca
     4021 gcctgcctgc cggcggcgcc ggggtcgaac gctcgctgcc gatcgtggtg ttcctcggcg
     4081 gcatcgtgac gagcctgctg ctgttctggg tcgtgtggac gttgctcgtc tcacgcgagc
     4141 gcgcgttgaa gctcgccgag cacgcccggg cggcgcgcgc ggcggagggg ctgcgggaac
     4201 agctcgcgtt catccagcag ctgatcgaga ccgtgccgca gcccatcttc ttcaaggacg
     4261 ccgagcagag gcgttacctg ggggtcaaca aggcctggga aaacttcttc ggcatcccgc
     4321 gcgagcagtt catcggaaaa accgtgttcg agctctatcc ccacaaccag gagctggcgc
     4381 agcgacatca tgccaaggac gaggagctgt tcagccgccc gggcagccag tcctatgagg
     4441 ccgcgatcgt cgcggcggac gggcgcgtgc atcacacgat ctacaacaag gcgaccttca
     4501 acaaatcgga cggctccgtc gcggggctga tcggaaccat caccgacgtg accgggctca
     4561 aggaagcgga agcggcgctg cgggagagcg aagcgcgctt ccgcgacctg accgaattgt
     4621 cctcggactg gtactgggag caggacgcgg aactgcgctt cacccagatt tcctcgaagg
     4681 ggcgcgaggt cagcctgggc gcggaggagg acatcggcaa gcggcgctgg gaggttgccc
     4741 gcctggacat gaccgaggag cagtgggtgc cgcacaaggc ggggctcgcg gcacggcagt
     4801 cgttccacga tttcacgtac cagcgccacg acacgaacgg cgagctgcgc atcatcagcg
     4861 tgagcgggcg gccgatcttc gacgagcggg gacgcttcaa gggctaccgc ggcaccgggc
     4921 gcgacatcac cgagcagaag cgcgccgagg agaggatccg gcacatggcg caccacgacg
     4981 cgctgaccgg gttgcccaac cgcgcgctgc tgcacgaccg catcgggcag gcgatcgtgc
     5041 aggccgagcg caatcccagg gtgttcgcgc tgctgttcat cgacctggac cggttcaaga
     5101 cggtcaacga ctcgctcggg caccaggtcg gcgaccggct gttgaagacg gtcgcggaac
     5161 ggctggtggc gtgcacccgg ggatcggaca cgattgcccg catcggcggc gacgaattcg
     5221 tggtgctgct cgacgatctc aaccagcccg aggacgcccg gcacgtggcg cagaaggtgc
     5281 tcgagacgct gtcggagccg gtgatgctcg acacgcacga gctgaaggtg acgcccagcg
     5341 tcggcatttg cgcctacccc catgacggca aggatgtcga gacgctgatg cgcaacgccg
     5401 acatcgcgat gtaccacgcg aaacagatgg ggcgtaacaa ctaccagttc ttcacccagg
     5461 cgatgaacga cgccgcacag cagcgactgc agctggaaaa cgacctgcgc cacgccgtcg
     5521 cgcgcggcga attcacgctg cattaccaac cgcagctcga cctggggacg ggagccatca
     5581 tcggattcga ggcgctggtg cgctggctgc atccgagccg cggcctgatc gcgccgtccc
     5641 agttcatccc ggcggccgag gaaaccgggc tgatcgcggc catcggggag tgggtgctgc
     5701 gcgaggcctg ctcgcgctcc cgcgcctggc atgaggcggg ccacccgcag ctgcaggtgt
     5761 cggtcaactg ctcggcccag cagtttcagc gcgagggctt cgtggagacc gtgggacgca
     5821 tcctgggcga aacggggctg cccgcggagc gcctcgatct ggagatcacg gagagcgtca
     5881 tcatccagcg ctccgaggaa gtgatcgcac ggttccaggc gctcgatgaa atgggcgcgc
     5941 gcatctcgat cgacgacttc ggcaccggct actcgagctt gagctatctc aagcgcttcg
     6001 cgatccatcg gctgaaaatc gatcagtcgt tcgtgcgcga catcagctcg gaccccgatg
     6061 acgcggccat cgtgagcgcc atcatcgcca tcgcgcacag cctcggcctc gaggtggtgg
     6121 cggaaggggt ggagacgtcc gaacagctgg cgttcctgaa atcgctggga tgcgacgcgg
     6181 cgcagggcta
//
LOCUS       DBNGWZ010000604         4851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_847148, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000604 DBNGWZ010000000
VERSION     DBNGWZ010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_847148"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="AC16CX_18720"
     CDS             complement(<1..800)
                     /locus_tag="AC16CX_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR3014576.1"
                     /translation="MSLPHYEVFAIRYARRDARRAEHFVGGDPHDAPMAMDYFVWLIR
                     NAERTIVVDTGFTAEVAARRKREFIRAPKEGLALLGVKAEAVRDVIITHMHYDHVGTF
                     FDFPNAVFHIQDDEMSFVTGRYMRYTRFAHSFEPEDVVGMVRLLFKGRVAFHKGAEEF
                     APGVSLHHIGGHTPGLQSVRVHTQRGWVVLASDATHYYEHMETGRCFPSIFNLGHVLE
                     GYETLRRLADSPRHIIPGHDPLVMARYPAVSKELEGIAVRLDVMPVEQR"
     gene            complement(812..1735)
                     /locus_tag="AC16CX_18725"
     CDS             complement(812..1735)
                     /locus_tag="AC16CX_18725"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014577.1"
                     /translation="MRIGFIGLGTMGSSMALNAHTQGGYDLVVHDLRRDAAAPHLAAG
                     AVWADSPAAVAKDADIVLTSLPGPRDVEAVAEGLIQTMRPGVPWFDLSTNSPTVMRRL
                     HERFAAKGLPVLDAPVSGGPKGAKSGKLALWVGGDRAVFDKFRAVLDSIGDQVRYIGP
                     IGAGSIAKLVHNCAGYAIHCAVAEVFTMGVRAGVEPLALWSAIRQGALGRKRTFERMA
                     DNFLQGRFDPPEFALNLAYKDVTLATELAREIGVPMRLASLAHAEMGEAINRGWGARD
                     SRVFLLLQEERAKVDIKVPAAEIQSVIEREG"
     gene            1881..3902
                     /locus_tag="AC16CX_18730"
     CDS             1881..3902
                     /locus_tag="AC16CX_18730"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3014578.1"
                     /translation="MLIARPGRMGPLRLRNRIIMGPMGTNFGTTDGLSTERDKLYYGE
                     RARGGAAMVITEAMVIQPGARNHTNSLCLFHDRYIPGLASVVDAIHEGGALAVGQVNH
                     RGALLKRSVLGMEPVSASDWHNPNTGEPARAVTIPEIEAIQRAYLDSARRLYRAGYDA
                     VELHAANGYLFQQFFSPRINRRQDRYGGSLENRMRFLLETVALVRGELPGFPLMVRVS
                     VTEYCEGGYSEPDMIALCQALERAGVIAIDMSGGTNESPQLSRYCIQPPSFARRFLEP
                     YARPIAKAIGIPTIMAGRVIDPEDAEGILRANSADFVAVCRALVADAYWPRKAFGEIK
                     TPIRKCISCNVCYERLTLERDVSCVTNPMVGLEFERIEMAEPQLRKDTVRQHRAGRKR
                     ILVLGAGVAGVEAARVAAALGHQVEIWEKAAQPGGQMPLALAAPDKADVAGVWTYRWQ
                     QIESQRVPVRTGVIATAATIRDFRPDLVVVATGAKPRPLPFPVKTRVPVLQAWDALLE
                     QGRVPRSARVTIIGGGMAGIETTDTLIHNRGIKATVIEALPVIAREMARNNRYDVLNR
                     LEKSGARVLVNAPVESVEGDMVWATVGGARTPIDAGELIIAAIGPLPNRDIVPEVERS
                     GAPYALVGDCNQIGNFLTAVRDGWMLALAVDMRLPEYRRWQAAVNSPTL"
     gene            4053..>4851
                     /locus_tag="AC16CX_18735"
     CDS             4053..>4851
                     /locus_tag="AC16CX_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014579.1"
                     /translation="MLFPATLVGSYPQPEWLIDRKKLAGRFPPRVRAKELWRIPEPYL
                     AEAQDDATVMAIEAQEEAGLDIITDGEIRRESYSNRFATALEGVDIDNPGTALDRSGH
                     PNPVPRIVGKIRRKHPVEVEDLEFLKRHTTRMVKITVPGPFTMSQQAQNDFYRHEEEA
                     AMDYAAAVNEEIRDLFAAGADIVQVDEPYMQARPEKARQYGLRALNRALEGIRGATAV
                     HICFGYAAVIHQRPSGYSFLPELADCACQQVSVETAQSNLDCSVLAKL"
BASE COUNT          821 a         1647 c         1629 g          754 t
ORIGIN      
        1 cgctgttcga cgggcatgac gtcgagccgg acggcgatgc cttcgagctc cttcgagacc
       61 gccgggtaac gcgccatcac cagcggatcg tggccgggga tgatgtgccg gggcgaatcc
      121 gccagccggc gcagcgtctc atagccctcg agcacgtgtc ccaggttgaa gatagacggg
      181 aagcagcgcc ccgtttccat gtgctcgtaa tagtgggtcg catccgaggc gagcactacc
      241 cagccgcgct gcgtgtggac ccgcaccgat tgcaggcccg gcgtatggcc gccgatgtga
      301 tgcaggctca cgccgggagc gaattcctcc gcgcccttgt ggaatgcgac gcggcccttg
      361 aacagcagcc gcaccatccc gaccacgtcc tccggctcga atgagtgcgc gaagcgtgtg
      421 tagcgcatgt agcggccggt gacgaagctc atttcgtcgt cctggatgtg gaagacggcg
      481 ttcgggaaat cgaagaacgt cccgacgtgg tcgtagtgca tgtgcgtgat gatgacgtcg
      541 cgcaccgctt ccgccttgac gcccagcagg gccagccctt ccttcggcgc gcggatgaac
      601 tcgcgcttgc gccgtgcggc gacctccgcc gtgaaaccgg tatccacgac gatcgtgcgc
      661 tcggcgttgc ggatcagcca cacgaagtag tccatcgcca tcggcgcgtc gtgcgggtcg
      721 ccgccgacga aatgctccgc gcggcgcgcg tcgcgccgcg cgtagcggat cgcaaacacc
      781 tcgtagtggg gaagcgacat gctcgacctc atcagccttc acgctcgatc accgactgga
      841 tctccgccgc cggcaccttg atgtccacct ttgcgcgctc ctcctgcaac agcaggaaca
      901 cccgcgagtc ccgggcgccc cagccgcggt tgatcgcttc gcccatttcc gcgtgggcga
      961 gcgacgcaag gcgcatcggc acgccgatct cgcgcgccag ctcggtggcg agcgtcacgt
     1021 ccttgtaggc aaggttgagc gcgaattccg gcggatcgaa cctgccctgc aggaaattgt
     1081 cggccatgcg ctcgaaggtg cgcttgcgcc cgagggcgcc ctggcggatc gccgaccaca
     1141 gcgccagcgg ctcgacgccg gccctgacgc ccatggtgaa gacttcggcc accgcgcaat
     1201 ggatcgcgta gcccgcgcag ttgtgcacca gctttgcgat cgagccggcg ccgatcgggc
     1261 cgatataccg cacctggtcg ccgatggaat cgagcaccgc gcggaacttg tcgaacaccg
     1321 cgcgatcgcc gcctacccac agagcgagct tgccgctctt cgcgcccttc ggcccgccgc
     1381 tcaccggggc gtcgagaacc ggcaacccct tggcggcaaa gcgctcgtgc aagcggcgca
     1441 tcacggtcgg cgaattggtc gagagatcga accacggcac gccgggccgc atcgtctgga
     1501 tcaggccttc cgcgaccgct tcaacgtcgc gcggccccgg caacgaggtc agcacgatat
     1561 ccgcatcctt ggccaccgcc gcaggcgagt cggcccagac cgcgcctgcc gcgagatgag
     1621 gcgcagccgc gtcgcgtcgc aggtcgtgaa cgacgagatc gtacccccct tgggtgtgcg
     1681 cgttcagcgc catgctgctg cccatggtgc ccaagccgat gaagccaatt cgcatatact
     1741 ctcctttcgg tcgcgttccg cggtgtgccg tcgggtccac gccgcggact cgccaactgt
     1801 gagttgcgaa gcctaccacg cgccgcgcta tcatcgcgaa gattgacgca cgctacgtcc
     1861 gcgtggtgga acccccagaa atgcttatcg ctcgaccggg ccgcatgggc ccgctgcggc
     1921 tgcgcaaccg catcatcatg ggcccgatgg ggaccaactt cggcaccacc gacggattgt
     1981 ccaccgagcg cgacaagctc tactacggcg agcgcgcgag gggcggagca gcgatggtca
     2041 tcaccgaggc catggtgatc cagccggggg cgcgcaacca taccaattcc ctgtgtctct
     2101 ttcacgaccg ctacattccc ggtctcgcca gcgtcgtgga tgcgatccac gagggcgggg
     2161 cgcttgcggt cgggcaggtc aatcaccgcg gggcgctgct caagcgctcg gtgctgggga
     2221 tggagccggt ttcggcctcc gactggcaca atcccaatac cggcgagccg gcccgggcag
     2281 tcaccatccc ggagatcgaa gcgatccagc gggcctatct cgattccgct cgccgcctct
     2341 atcgcgccgg gtacgacgcg gtggagctgc acgccgcgaa cggctacctg ttccagcagt
     2401 tcttcagccc gcgcatcaac cggcgccagg accgctacgg cgggtcgctc gagaaccgca
     2461 tgcgcttcct gctggaaacg gtcgcgctgg tgcgcggtga gctgcccggc ttcccgctca
     2521 tggtgcgggt cagcgtgacc gagtattgcg agggcggcta tagcgagccg gacatgatcg
     2581 cgctgtgcca ggctctggag cgcgccgggg tcatcgcgat cgacatgtcg ggcggcacca
     2641 acgagagccc gcagctgtcg cgctactgca tccagccgcc gtccttcgcg cggcgctttc
     2701 tcgagccgta cgcgcggccg atcgcgaagg cgatcggcat tccgaccatc atggcgggcc
     2761 gcgtcatcga cccggaggac gcggagggca tactccgggc gaacagcgcc gatttcgtgg
     2821 ccgtctgccg tgcgctggtg gccgacgcct actggccgcg caaggccttc ggcgagatca
     2881 agacgcccat ccgcaagtgc atttcctgca acgtgtgcta cgagcgcctg accctggaac
     2941 gcgatgtgtc ctgcgtgacc aatccgatgg tcggcctcga gttcgagcgc atcgaaatgg
     3001 cggagccgca gctgcgcaag gacacggtgc gtcaacaccg ggcaggacgc aagcgcatcc
     3061 tcgtgctggg cgcgggcgtg gccggagtcg aggcggcgcg cgtggccgcg gcactcggtc
     3121 atcaagtcga aatctgggaa aaagccgcgc aaccgggcgg ccagatgccg ctcgcgctgg
     3181 ccgccccgga caaggccgac gtcgcgggcg tctggaccta tcgctggcag cagatcgaga
     3241 gccagcgcgt gccggtgcgc accggcgtga tcgcgactgc cgcgacgatc cgggatttcc
     3301 ggcccgacct ggtggtggtc gcgaccggcg cgaagccccg gcccctgccg ttcccggtga
     3361 aaacgcgcgt cccggtgctg caagcctggg acgcgctgct cgagcagggg cgcgtgccgc
     3421 gaagcgcgcg cgtcacgatc atcggcggcg gcatggccgg aatcgaaacg accgacaccc
     3481 tgatccacaa ccgcgggatc aaggccacgg tgatcgaggc cctgccggtg atcgccaggg
     3541 agatggcccg caacaatcgc tacgacgtgc tgaaccgcct ggaaaagagc ggcgcgcgcg
     3601 tgctcgtcaa cgccccggtg gagtcggtgg agggtgacat ggtctgggcg acggttggag
     3661 gcgcgcgcac gccgattgat gcgggcgagc tgatcatcgc ggccattggg ccgctgccca
     3721 accgcgacat cgtcccggaa gtcgagcgct cgggcgcgcc ctacgcgtta gtgggtgact
     3781 gcaaccagat cgggaacttt ctgaccgcgg tgcgcgacgg gtggatgctc gcgctcgccg
     3841 ttgacatgcg tctccccgag taccggcgct ggcaagcggc ggtgaacagt cctacacttt
     3901 gagccccgga aaaccggtcc atgcaacgaa aggagaagtc cacatggtgc gcatcggagt
     3961 cctgatctcc atcgtcctcg cggcggcagc tttgccgcgg agatcgtcgc ccgcattgat
     4021 taacacgggg tgacgtggtg ccggaaagcg cgatgctgtt tccggcaacg ctggtcggga
     4081 gctacccgca gcccgagtgg ctgatcgacc gcaagaagct ggcggggcgt ttcccgccgc
     4141 gcgtgcgcgc gaaggaactg tggcgcatcc cggagccgta cctggcggag gcgcaggacg
     4201 acgccaccgt catggcgatc gaggcgcagg aggaggcggg cctcgacatc atcaccgacg
     4261 gcgagatccg gcgcgagagc tactccaacc gctttgccac cgcgctcgaa ggggtggaca
     4321 tcgacaatcc cggcaccgcg ctcgaccgct cgggccatcc caacccggtg ccgcgcatcg
     4381 tcgggaaaat ccgccggaag cacccggttg aagtcgagga tcttgagttc ctgaagcgcc
     4441 ataccacccg gatggtgaag atcaccgtgc ccgggccgtt caccatgtca cagcaggcgc
     4501 agaacgactt ctacaggcac gaggaggaag cggcgatgga ctacgccgcc gcggtcaacg
     4561 aggaaatccg cgatcttttc gccgccggcg cggatatcgt gcaggtcgac gagccctaca
     4621 tgcaggcgcg gccggagaag gcgcgccagt acggactgcg ggcgctcaat cgcgcactgg
     4681 agggcatccg gggcgcgacc gcggtgcaca tctgcttcgg ctacgccgcg gtgatacacc
     4741 agcgcccctc gggctactcc ttcctcccgg agctggcgga ctgcgcgtgc cagcaggttt
     4801 cggtcgagac cgcgcagtcc aatctcgatt gctcggtgct ggcgaagctc g
//
LOCUS       DBNGWZ010000605         7787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3369576, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000605 DBNGWZ010000000
VERSION     DBNGWZ010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3369576"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16CX_18740"
     CDS             complement(<1..227)
                     /locus_tag="AC16CX_18740"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3014580.1"
                     /translation="MFTRIDHVMICVPDLQQGMAQYRRMGFNVHPGGVHPGKGTHNAI
                     ALNQEDYIELLAIRDPAEHEAAGRAGSWGADL"
     gene            complement(468..932)
                     /locus_tag="AC16CX_18745"
     CDS             complement(468..932)
                     /locus_tag="AC16CX_18745"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014581.1"
                     /translation="MPRVLPLLLFIIAATIAPALHAVDTEEQPGAAALAPDYAAGKKA
                     IEAKDWSAAIKHFSSAALRAPDNADIQNYLGYAYRNAGKLDAAFQHYQRALELNPRHR
                     GAHEYIGEAYLMVKNLAKAEEHLAALDRICLLPCEEYSDLKAKVTAYKKGAR"
     gene            complement(944..2521)
                     /locus_tag="AC16CX_18750"
     CDS             complement(944..2521)
                     /locus_tag="AC16CX_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014582.1"
                     /translation="MRSSISICAFIAALSAAIPAHGAEQLGKVSFPTSCDPKVQAQVE
                     RGVAMLHSFWYSATLKTFQDVLAQDPSCAIANWGIAAILMDNPLAGVGASPKGAEQAR
                     AAIEQGRAIGAKTQRERDYIEAVAAYYEDWANRFERDRQQSRAKAFEALAARYPNDDE
                     AQIFYALYLAGTQSQADQTFAAYLKAAAILEKQFAKYPDHPGVAHYLIHSYDAPPIAQ
                     RGLPAARLYAKIAPAAPHALHMPSHIFTRVGAWTESVETNRRSAVTAIQGNEPDGALH
                     AMDYTVYAYLQLARDADALRTAEEAERVTGVNYGRFVGTYALAAMRARYAIERGDWSG
                     AMKLQPRAGKFPYSDALTHFARALGAARSGDPAAAEKDVKQLESLRDALKAAKNTYWA
                     TEVEINRLGAAAWTALAQGRKEEALSLMRSAADTEDKNEKHIVTPGRLAPARELLGEM
                     LLELKQPALALKEFEASQIREPNRFRGFYGAAQAAEASGDRQKAADYFAKLVAMAKDS
                     DTARPELRRAKAYLAAR"
     gene            complement(2931..3935)
                     /locus_tag="AC16CX_18755"
     CDS             complement(2931..3935)
                     /locus_tag="AC16CX_18755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014583.1"
                     /translation="MTGIGNLLFAGFLAIGAAGAPTAGAAEPQGKAEAAYPSKPIRII
                     SPLASGGSADAIARMVGETLADAFGQPVVVDNRPGANGLIGVDIVAKATPDGYTLLVA
                     SGSNLAINPALRGSSLPFDVERDLVPIMQIASQTFIAHVSPSFSAKSIRELIALAKAR
                     PGAINYASAGAGSTAHLMAALFESMAGVRMTHVPYKGAAQGRIAVISRECDLMFDGLL
                     AALPLIKAGRLRGLGVTSAKRSGVAPDIPTIAEAGVPGYGADAWYGLLAPRGTPGSIV
                     AKLWATLNKSLEASKTREKLLAQGVEPVGSTQKEFRAFLKAEIVMWARVVKEAGVKPE
                     "
     gene            complement(3996..5051)
                     /locus_tag="AC16CX_18760"
     CDS             complement(3996..5051)
                     /locus_tag="AC16CX_18760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR3014584.1"
                     /translation="MPAGKTQKTVFWFDDGKRPNVADSLEKETDIVLHRLAFNGPKAD
                     NWGAMSASHVYCITSTRQELPEEYRANAALIARCPDLLAVSASGAGYDTVDVAACTAA
                     GVLVVNQSGGNADAVAEHTVAMMLSLTKNIPQTDRSVRAGKRAPRESFKGWNAKGRTV
                     GLVGIGNVGSRVARICGPGLQMRVLACDPYLTAEEIQARGATKVDLATLLAESRFVSI
                     HCPYNDETRNMIGARELAAMQPGSFLITTARGGIVDENALATALESKHIAGAGVDVWI
                     EEPPPLSHPLLAFDNLIATYHTAGITHDSRNNLADWNAQQVAGILRGERPPRLVNPEA
                     WERFAQRFERIFGFHPQ"
     gene            5278..6939
                     /locus_tag="AC16CX_18765"
     CDS             5278..6939
                     /locus_tag="AC16CX_18765"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virginiamycin B lyase family protein"
                     /protein_id="HIR3014585.1"
                     /translation="MRITHFLFAVLAALILTLFPAGPAAAQADALTGQVSSAAEGPME
                     GVLVSARRAGSNATVTVVTDAQGRYRFPAARLEPGKHTLSTRAAGYDLARRATVEVAA
                     GATATTDLKLIPTRDIAAQLTNAEWLHSMPGTPQQKRPLLGCVGCHTLERVARSRYDT
                     EGFVRTIQRMSTYANQSTPLHPQRRLTTRNTDVVGEERARIQWAQAEYFSTINLSAHT
                     TWEYDLRTFPRPKGRATRVIITEWDLPRPTIEPHDVVVDSKGMAWFSNFGEQTIGKFD
                     PRTGTLTEIPVPEFKKGSPTGALSVRMDRDENVWLGLMYQAGFARLDTRTEKLQVWNI
                     PAELNKDNTQINMTSPMNIGVDGKIWAQNNGFAGVHRVDLKTGKWETWDPFKTSPTGH
                     NIYDVISDSQNNAYFTDIGKEHIGRIDAKTGKVTFYETPTKSSGPRRGMMGAQDRLWF
                     GQYRGNRIAMFDTRSERFQEWEVPTPWSGPYDVAVDKNGEAWTGSMLSDRVARLDPRT
                     GQFVEYLLPRPTNIRRVFVDSSTTPVTFWVGSNHGASIIKLEPLD"
     gene            7086..7529
                     /locus_tag="AC16CX_18770"
     CDS             7086..7529
                     /locus_tag="AC16CX_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014586.1"
                     /translation="MKPIRVGVAAMTAVALQAAAAPAAEPALDYEFFKARVQPIFLKK
                     KPGHTRCVVCHAESNNAFRLQKLLPGAKSWTEEQSRANFEMASKLVVPGNLAASRLLI
                     HPLAPEAGGGAFHNGGRQFATKNDPDWKTLNAWANGKKLAAAKSK"
     gene            complement(7610..7771)
                     /locus_tag="AC16CX_18775"
     CDS             complement(7610..7771)
                     /locus_tag="AC16CX_18775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014587.1"
                     /translation="MPNWTTFTPDGRYLYVTNSAINSVSVVDTKAMKVVANVRVGQVP
                     KRIGTLALR"
BASE COUNT         1367 a         2541 c         2539 g         1340 t
ORIGIN      
        1 aggtcggcgc cccagctacc ggcccttccc gccgcctcgt gctcggccgg atcgcggatc
       61 gcgagcagct cgatgtagtc ctcctggttg agcgcgatgg cgttatgggt gcccttgccg
      121 gggtgaacgc cgcccggatg gacgttgaag cccatcctgc ggtactgcgc catcccttgc
      181 tgcaggtcgg ggacgcagat catgacgtgg tcgatgcgtg tgaacatggc cggcagttta
      241 cgcttcgctg aaggatgaag gatgagggat gaaggatgac cttgagacca gcgactcgcg
      301 ggtcgtgact aggccgactc gatgccgcgt cgaatcacga cccacgaatc ccgttccaat
      361 tacctccgcc agttcgtcat gattgatggt ccgtgacatg cctggaagac gaccattgga
      421 acgggacgaa tcacgatttt ctcccgcacg agcgcgcggg acagtgctta cctggcgcct
      481 ttcttgtaag cggtgacctt ggccttgagg tcggagtatt cctcgcaggg cagcaggcag
      541 atgcgatcca gcgccgcgag gtgctcttcc gccttggcga ggttcttcac catcaggtac
      601 gcctcgccga tgtactcgtg cgcgccgcgg tggcgcgggt tcagctcgag cgcgcgctgg
      661 tagtgctgga acgcggcgtc gagcttgccg gcattgcgat aggcgtagcc gagataattc
      721 tggatgtcgg cattgtcggg ggcgcgcagc gctgcggatg agaagtgctt gatcgcggcg
      781 ctccagtcct tcgcctcgat ggctttcttg cccgccgcat agtcaggggc gagcgcagcc
      841 gcgccgggct gctcctccgt gtccacggcg tgcagcgccg gcgcgatcgt cgcggcaatt
      901 atgaaaagca gcaaaggcaa tacgcgcggc atcgaatgcc aggctacctc gccgccaaat
      961 atgccttagc gcgccggagt tccggccgcg cagtatcgga gtcctttgcc atggccacga
     1021 gcttcgcgaa atagtccgcc gccttctgcc ggtcgccgga cgcctcggcg gcctgtgccg
     1081 ccccgtagaa gccgcggaac cggtttggct cgcgtatctg cgatgcctcg aactccttga
     1141 gcgccagcgc cggctgcttg agctcgagca gcatttcgcc gaggagctca cgcgcgggag
     1201 cgagtcgtcc cggggtcaca atatgttttt cgttcttgtc ctcggtgtcc gccgcgctgc
     1261 gcatcaggct gagcgcttcc tctttcctgc cttgtgcgag agccgtccac gcggcggcgc
     1321 caagccggtt tatctccacc tcggttgccc agtacgtgtt tttcgccgct ttcagcgcat
     1381 cgcgcagcga ttcgagctgc ttcacgtcct tttccgctgc ggcggggtcg ccgctgcggg
     1441 cggccccgag cgcgcgtgcg aagtgggtca gcgcatcgct gtacggaaac ttgcccgcgc
     1501 ggggttgcag cttcatcgct ccgctccagt caccgcgctc aatcgcatag cgcgcccgca
     1561 ttgccgcgag cgcgtaggta ccgacaaaac gcccgtagtt gacgccggtc acccgctccg
     1621 cctcctccgc ggtgcggagc gcatccgcat cacgcgcaag ctggaggtag gcgtagaccg
     1681 tgtaatccat ggcgtgtagc gcgccgtcgg gctcgttgcc ctggatagcg gtcaccgccg
     1741 agcggcggtt ggtttcaacg gattccgtcc acgcgccgac acgggtgaag atgtgcgaag
     1801 gcatgtgcag ggcgtgcggc gccgccggcg cgatcttcgc gtagagccgc gcggcaggca
     1861 ggccccgctg cgcgatcggc ggggcatcgt agctatgtat caggtagtgc gccacgccgg
     1921 ggtggtcggg gtacttggcg aattgttttt cgaggatcgc ggccgccttg agataggcgg
     1981 cgaaggtctg atcagcctgc gattgcgtgc ccgcgagata cagcgcgtag aagatctgcg
     2041 cttcgtcgtc attcgggtag cgggcggcaa gcgcctcgaa ggccttggcg cgcgactgtt
     2101 gacggtcgcg ctcgaagcgg ttggcccaat cctcatagta ggcagcgacc gcctcgatgt
     2161 aatcgcgctc gcgctgggtc ttggcgccga ttgcgcgccc ctgctcgatg gccgcccgcg
     2221 cctgctccgc acccttggga gaggccccga ctcccgcaag tgggttgtcc atcaaaatcg
     2281 ccgcgatgcc ccagttcgcg atcgcgcaag agggatcctg cgccaagacg tcctggaagg
     2341 tcttcagggt cgcgctgtac cagaacgagt gcagcatcgc cacgccgcgc tcgacctgag
     2401 cctgtacttt gggatcgcag gacgtgggaa agctgacctt gcccagctgc tccgcgccat
     2461 gcgccggaat tgccgcggag agcgctgcga taaacgcaca aatcgagatg gaagatctca
     2521 tgattgactc cttgatccag gttgtgagca gattccaggt atttccgccg gccggaaatt
     2581 cgttatcgtc acaccaggag tcaagtgaga acagaccgag ctgacgcgag tcgcacggta
     2641 ggctcaagca ggatacacgc tttatgtgcc aattacgttg agcagtcatt tatattttcg
     2701 gccccggctt tcgtccggca cggcctctcc tccccacaag ctcgaagagc gtgactcgag
     2761 ttcagtgctg agttccgagt gctgaggggc ccgagttggg ggcggatcag gaaaatccct
     2821 acaagcggga tgggggatta ggactgagtg ctgagggccc taatcccggt gacgactgac
     2881 gacaaacacc ggcgaggagt ggggggctac actggcccca gttgttggct ttactcaggc
     2941 ttcacgcccg cctccttgac gaccctggcc cacattacga tctcggcctt gaggaaagcg
     3001 cggaattcct tttgcgtgct gccaacgggt tcaacgccct gcgcaagcaa cttttcccgc
     3061 gtcttggacg cctccagcga cttgttgagg gtcgcccaca gtttcgcgac gatgcttcct
     3121 ggcgtgccgc ggggcgcgag caggccgtac cacgcgtcgg cgccgtagcc ggggacgccg
     3181 gcctcggcga tcgtcgggat gtcgggcgca acgccggagc gtttcgccga ggtgacgccc
     3241 aggccgcgca gcctgcccgc cttgatcagc ggcagggccg ccagcaggcc atcgaacatc
     3301 aggtcgcact cacgggatat caccgcaatg cggccctggg cggcgccctt gtagggcacg
     3361 tgggtcatcc tgaccccggc catgctttcg aacagggccg ccatcaggtg cgcggtcgag
     3421 cccgcgcccg ccgaagcgta gttgatggct cccggcctgg ccttcgcgag cgcgatcagt
     3481 tcccggatgg atttcgcgga aaacgacggg gacacgtggg caatgaaggt ctgtgacgcg
     3541 atctgcatga tcggaaccag atcgcgctcg acatcgaacg gcaggctgga accacgcaga
     3601 gcgggattga tcgcgaggtt gctgcccgaa gcgaccagca gcgtgtagcc atcgggcgtt
     3661 gccttggcga cgatatcaac gccgatcagg ccgttggcgc cgggccgatt gtcgactacg
     3721 acgggttggc cgaacgcgtc cgcgagcgtc tcgccgacca tacgcgcaat cgcgtcggcg
     3781 ctaccgccag aggcaagcgg cgaaatgatc cgaatcggtt tcgacggata agccgcttcg
     3841 gccttccctt gcggctcggc cgcgcccgca gtcggggcgc ctgccgcccc gatcgcaaga
     3901 aacccggcaa acagaagatt tccgattcca gtcacaactg tttttcagcc tgcttgcctt
     3961 atttgactcc gctcaccggt cactactcac ggtggttatt gcgggtgaaa gccgaagatc
     4021 cgctcgaagc gctgcgcgaa acgctcccac gcctccggat tgaccagccg cggtgggcgc
     4081 tcaccccgca ggatgccggc gacctgctgc gcgttccagt cggccaggtt gttccgggaa
     4141 tcgtgcgtga tgccggcggt gtggtaggtg gcgatcaggt tgtcgaacgc gagcaacggg
     4201 tgcgagagcg ggggcggctc ctcgatccag acgtccaccc ccgcgccggc gatgtgcttc
     4261 gactcgagcg ccgtggcgag ggcgttctcg tccacgatcc cgccgcgcgc tgtcgtgatc
     4321 aagaaggacc cgggctgcat ggcggcgagc tcgcgggccc cgatcatgtt gcgggtctcg
     4381 tcgttgtacg ggcagtggat cgagacgaag cgcgactcgg cgaggagcgt cgcgagatcc
     4441 accttggtcg cgccccgcgc ctggatctct tccgccgtga ggtagggatc gcacgcgagc
     4501 acccgcatct gcagccccgg gccgcagatg cgcgcgacgc ggctgccgac gttgccgatg
     4561 ccgaccaggc caaccgtgcg gcccttggcg ttccagccct tgaaggactc ccgcggcgcg
     4621 cgcttgccgg cgcgcacgga gcggtccgtc tgcggaatgt tcttggtgag cgagagcatc
     4681 atcgccaccg tgtgctccgc cacggcatcg gcgttgccac ccgactggtt caccacgagc
     4741 acgcccgccg ccgtgcacgc cgccacgtcc accgtgtcgt agcccgcgcc gctcgccgac
     4801 acggcgagga gatcggggca gcgcgcgatc agcgccgcat tggcgcggta ctcttcgggc
     4861 agctcctggc gcgtcgaggt gatgcagtac acgtgcgagg ccgacatggc gccccagttg
     4921 tccgctttcg gcccgttgaa ggcgaggcgg tgcaggacga tgtctgtttc cttctcaagc
     4981 gagtcggcga cgttcggccg cttgccgtcg tcgaaccaga acacggtctt ttgggtcttg
     5041 ccggcgggca tatgaagtcc gttgcgcagg ggttggtaaa cggcgggatt ctaactggga
     5101 atgtcgtcac gggtgacgag tcaccagccg tcatctcact catcacttac cacgccgttt
     5161 aaagccaagc cagtaggcat ccgcccgcgc cggtgctacg atggggcggc agtcgcttca
     5221 atccaataaa aaatcgtcga gggggtagct cagcaagcct tcaggagggg gctacccatg
     5281 cgcatcacgc atttcctgtt cgcggtgctc gccgcgctca tcctcacgtt gttcccggcg
     5341 ggtcccgcgg ccgcccaggc cgacgcgctc accgggcagg tctcctccgc cgcggagggc
     5401 ccgatggagg gcgtgctggt gagcgccagg agggcgggct cgaacgcgac ggtgaccgtc
     5461 gtgaccgacg cgcagggccg ctaccgcttc cccgcggcgc ggctcgagcc gggaaagcac
     5521 accctgtcca cccgcgccgc cggctacgac ctcgcgcgac gcgcgacggt cgaagtcgcg
     5581 gcgggggcca cggcaaccac cgacctgaag ctgattccga cccgggacat cgccgcccag
     5641 ctcaccaacg ccgagtggct ccacagcatg cccggcacgc cgcagcagaa gcgcccgctg
     5701 ctcggctgcg tgggctgcca cacgctcgag cgcgtcgccc gctcccgcta cgatacggaa
     5761 ggcttcgtgc ggacgatcca gagaatgtcg acctacgcga accagagcac gccgttgcac
     5821 ccgcagcgcc ggcttaccac ccgaaacacc gacgtcgtcg gcgaggaacg ggccaggatc
     5881 cagtgggcgc aagccgagta cttcagcacc atcaacctga gcgcgcatac cacctgggaa
     5941 tacgacctca ggactttccc gcgccccaag ggccgggcga cgcgcgtgat catcaccgag
     6001 tgggacctgc cgcgccccac gatcgagccg catgacgtgg tcgtcgattc gaaaggcatg
     6061 gcctggttct ccaatttcgg cgagcagacc atcggcaagt tcgacccgag aaccggcacg
     6121 ctcaccgaga ttccagtgcc cgagttcaag aagggctcgc cgaccggcgc gctgtccgtc
     6181 cggatggacc gcgacgagaa cgtatggctc ggcctcatgt accaggcggg cttcgccagg
     6241 ctcgacacca ggaccgagaa gctccaggtc tggaacattc ccgccgagct caacaaggac
     6301 aatacccaga tcaacatgac gagcccgatg aacatcggcg tggacggcaa gatctgggcc
     6361 cagaacaacg gcttcgccgg cgtgcaccgg gtggacttga agaccggcaa gtgggagacg
     6421 tgggacccgt tcaagacgtc gcccaccggg cacaacatct acgacgtgat ctcggactcg
     6481 cagaacaacg cctatttcac cgacatcggc aaggaacaca tcggccggat cgacgcgaag
     6541 acgggcaagg tcaccttcta cgagacgccg accaagagct cggggccgcg ccgcggcatg
     6601 atgggcgcgc aggaccgctt gtggttcggc cagtaccgcg gcaaccgcat cgcgatgttc
     6661 gacacgcgga gcgagcgctt ccaggaatgg gaggtgccga cgccgtggtc ggggccgtac
     6721 gacgtcgccg tggacaagaa cggggaggcg tggaccggct cgatgctgag tgaccgcgtg
     6781 gcgcggctcg acccgaggac cgggcaattc gtcgaatatc tcctgcccag gcccaccaac
     6841 atccgccgcg tgttcgtgga cagctcgacg acgccggtca ccttctgggt cggcagcaac
     6901 cacggcgcat ccatcatcaa gctcgagccg ctggactgat actccgtgac cggtgaagcg
     6961 tgactgagga ggcgtgactg atgaaccgtg gcagataagg gggattgaac gacccgcccg
     7021 ttaccgggtc gattcacgac tcacgaatca cgcattacga tccatcaaag aaagagggag
     7081 cccttatgaa accgattcgc gtgggagtgg cagcgatgac ggcggtcgcg ctgcaggccg
     7141 ccgcggcgcc agcggccgag ccggccctcg attacgaatt cttcaaggca cgcgtccagc
     7201 ccatcttcct caagaaaaag cccgggcaca cgcgctgcgt cgtctgccac gcggaaagca
     7261 acaacgcctt ccgcctgcag aagctgctgc ccggcgccaa gtcctggacc gaggaacagt
     7321 cccgtgcgaa cttcgagatg gcctcgaagt tggtggtccc gggcaacctt gccgcgagca
     7381 ggctcctcat ccacccgctc gctcccgaag ccggcggcgg cgctttccac aacggcggca
     7441 ggcagttcgc caccaagaac gacccggact ggaaaacgct caacgcgtgg gccaacggca
     7501 agaagctcgc ggcggcaaag agcaaatagg atcaaggaga gggatgaccc ccgcggtgcg
     7561 ggagtaaaac caggtcatcc ttccgccttc cgccttcatc cttcagttgt tagcggagcg
     7621 ccagcgtgcc gatccgcttc ggcacctgtc cgacccggac gttcgccacc accttcatcg
     7681 ccttggtgtc gacgacggac accgaattga tcgcggagtt ggtgacatag agataccggc
     7741 cgtcgggcgt gaaagtggtc cagttcggca acgccccgat cggctcg
//
LOCUS       DBNGWZ010000606         4642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2361411, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000606 DBNGWZ010000000
VERSION     DBNGWZ010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2361411"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="AC16CX_18780"
     CDS             complement(<1..1271)
                     /locus_tag="AC16CX_18780"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014588.1"
                     /translation="MDSMIERLAEYATGLRYEDLPQEAVHECKRKLIDTLGCLVGGFD
                     AGPSVIARKIARQSRGTPPARILGTQERSSPELAAFANGVALRYLDYNDAYFMKSSGH
                     PSDTFAAVLAAADAARADGRRVVTAAAAAYEAYCGISDAVKREQGWDHSVQAVIGSAV
                     GAGKVLGLGREAMGNAIALAVIPNMALEQTRTGELVMWKGCAGANAARNGVFAAQLAR
                     AGLTGPGQAIEGKWGLWHALGRFDWQPFGGRGGPYRITQTHIKYYPVVVHAQTPVTVA
                     LELHGTVDPRDIEAVAIVTYWVAERYADRGNPLWHPATRETADHSIPYCVAAALLDGG
                     ISAESFTEARIRDLRLTALIERTTLREDPDFTKAHPAEWPCRIELTLRGGKRKAAEVR
                     YFKGHAKRPLSDAELEEKFRTLAESALGKKRA"
     gene            1670..2320
                     /locus_tag="AC16CX_18785"
     CDS             1670..2320
                     /locus_tag="AC16CX_18785"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1991..1993,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR3014589.1"
                     /translation="MTSLNLTLVSPLPVYLDESGPQRKLAPRPASLDGKVVGLLPNWR
                     PSAVHILRAIGALLAERARLKAVVMEQPVRELPIRTGKLLDTMRGQLDDLAKRVDVVI
                     TATGDUGSCTTWCSHVTPALEERGIPTVMVVTDTFAKFARRMSSTQGWPYVIIAETQN
                     PIRQLEPDALRARAEAMIATVIEGLTLPPAEIESRLKNVARAEIHPEGVVRSAVPV"
     gene            2323..3342
                     /locus_tag="AC16CX_18790"
     CDS             2323..3342
                     /locus_tag="AC16CX_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014590.1"
                     /translation="MAQSQVQLQDESVEGLLARAYEHAYEQGWTDGLPIIPATPGSVQ
                     QFVTASGRAADEVIGLLPPRKGRATVEAIAVNAVMAGCRPEYMPVIIAAVEGMLDPSY
                     PLEFMQVTTNPMTPFLLVNGPVRKQLDINCGTGCLGPGWRANATIGRAIRLIMNNIGG
                     ALPGVYSKVSFGSPLRYSYICGENEEENPWTPFHVDRGFRREQSTVTVFKASNFCNIS
                     GGEGVGPAEILRQIATNMPPMYGGGDGVLLLLGVNHALALHEAGLAKRDIQSRLWEMA
                     RLPASYFAEDFAATERAAGRGDAETVWRARNPDEIYIAVAGGPGPQDVYIGAGMPQTR
                     VIRGA"
     gene            3365..3970
                     /locus_tag="AC16CX_18795"
     CDS             3365..3970
                     /locus_tag="AC16CX_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014591.1"
                     /translation="MRHSRLVFVLCGLAFIAAGLFPMIMALGIVPEWEEKMNAPRWVV
                     FLAGGLFFMAGMWLLLQAIVGEAVATAFGTVVGLAFFIGLAAVGHWMAFGGGDRSDCS
                     GGISALGFGFSTSTPDFECRAAFGYGALLMDFILLRGIAWWIAQRIPESRAVHAFEKA
                     AEWGIGLLLLPLVLLALLLSKGKDGVTTLVGKLRSNRNRQD"
     gene            4096..4536
                     /locus_tag="AC16CX_18800"
     CDS             4096..4536
                     /locus_tag="AC16CX_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3014592.1"
                     /translation="MYKNILLPTDGSALSDVAIDNGLKFAKAIGAKVTGFYVMIEQQP
                     ESFEDYAPVQVKAPKLEEVTKQEADKYLKVIADKARAMGVPCDTRSMKHASPHQAIIT
                     MATQTGCDLIVMASHGSKGITGELVGSETSRVITNCKIPVLVYR"
BASE COUNT          780 a         1598 c         1467 g          797 t
ORIGIN      
        1 gcccgcttct tcccgagcgc cgattccgca agggtgcgga atttttcctc cagctccgcg
       61 tcgctcagcg gccgtttcgc gtgtcccttg aaatagcgca cctccgcggc tttccgcttg
      121 ccgccgcgca gggtgagctc gatgcggcac ggccattcag ccgggtgtgc cttcgtgaaa
      181 tccggatcct cgcgcagggt ggtacgctca atcagcgcgg tcaggcgcag atcacgaatt
      241 cgcgcttccg tgaagctttc cgcgctgatt ccgccgtcga gcagcgccgc ggcgacgcag
      301 tagggaatgc tgtgatccgc ggtctcgcgc gtcgccggat gccatagcgg gttcccccgg
      361 tccgcgtagc gctccgccac ccagtaggtg acgatcgcga ccgcctcgat gtcccttggg
      421 tcaaccgtcc cgtgcagctc cagcgcgacc gtgaccggcg tctgcgcgtg cacgacgacc
      481 gggtagtact tgatgtgggt ctgcgtgatc cggtacggcc cgccgcggcc gccgaagggc
      541 tgccagtcga agcggccgag cgcgtgccac aggccccact tgccctcgat cgcctgcccg
      601 ggaccggtca gccctgcgcg cgcgagctgc gcggcgaaca cgccgttgcg cgccgcgttg
      661 gcgcccgcgc agcccttcca catgacgagt tcgccggtgc gggtctgctc gagcgccatg
      721 ttcgggatca ccgcgagcgc aatggcgttg cccatcgcct cgcgaccgag ccccaacacc
      781 ttgccggcgc ccaccgcgct cccgatgacg gcctgcaccg aatggtccca cccctgctcc
      841 cgcttgaccg catcggagat tccgcaatag gcctcgtagg cggccgcggc ggcggtcacg
      901 acgcgacggc cgtccgctcg cgccgcatcg gctgcggcca gcaccgcagc gaaggtgtcg
      961 ctcgggtgcc cgctcgactt catgaaatag gcgtcgttgt agtcgagata gcgcagcgcg
     1021 accccgttgg cgaaggccgc cagctcgggc gaggagcgct cctgcgtgcc gaggatgcgc
     1081 gcgggcggcg tgccgcggct ttgccgcgcg atcttgcgcg cgatgacgga cggcccggca
     1141 tcgaacccgc cgacgaggca gccgagcgtg tcgatgagct tgcgcttgca ctcgtggacg
     1201 gcctcttgcg gcaggtcctc gtagcgcagg cccgtcgcgt attccgctag ccgctcgatc
     1261 atcgaatcca taaaaaatgg cgtcggtagt ggacgatctt gcagcaattt cacctgcgga
     1321 ccaactgtaa tcgcaatcac caatgctact ccatcccgac gccatttttt taccgaccct
     1381 gttcttcccg atatatgacg tgaggcggaa gcaccggatg cagttcacct caatgagggt
     1441 tccgggccaa taaatactcc atttcagcct gtcggctccg aggtaaccag gatcgactac
     1501 ccatgtgctc acaccgatca ttcatgggac caagtccgac aggctgctgg catgccgcgc
     1561 gacgctgact ttcctcgagc caccccgcgc acagccgccg ttgcttgacc ctgcgcggag
     1621 cgccgaatta atatcgaagg cacgatacaa atcgtgcaag gaacacatta tgacatcgct
     1681 caacctgacc ctggtctcgc ccctgcccgt ctacctcgat gagagcggcc cccagcgcaa
     1741 gctcgcgccg cggcccgcaa gcctcgacgg caaggtggtg gggctcctgc ccaactggcg
     1801 cccctcggcc gtccacatac tccgcgccat cggcgcgcta cttgcggaac gcgcccgctt
     1861 gaaggccgtg gtcatggaac agccggtgcg cgagctgccg attcgcaccg gcaagctcct
     1921 cgatacgatg cgcgggcagc tcgacgatct cgcaaagcgc gtcgacgtcg tcatcaccgc
     1981 caccggcgac tgagggtcgt gcacgacgtg gtgtagccac gtcacgccgg ccctcgagga
     2041 gcggggcatc cccacggtga tggtcgtcac cgacacgttc gcgaagttcg cgcgccgcat
     2101 gtcgtccacg cagggctggc cgtacgtgat catcgccgag acgcagaacc cgatccggca
     2161 gctggagccc gatgcgctgc gcgcgcgcgc cgaagccatg atcgccacgg tgatcgaggg
     2221 gctcaccctt ccgcccgccg agatcgagtc gcgcctgaag aacgttgcaa gggccgagat
     2281 ccatcccgaa ggcgtcgtgc gttccgccgt gccggtataa aaatggccca atcccaggtt
     2341 cagctccagg acgaatcggt cgaggggctg ctcgcgcgcg cctacgagca cgcttacgag
     2401 cagggctgga ccgacggcct gccgatcatc cccgcgactc ccgggagcgt gcagcagttc
     2461 gtcaccgctt cgggccgcgc ggcggatgaa gtgatcgggc tcctgccgcc gcgcaagggc
     2521 cgcgcgacgg tggaagcgat cgcggtcaac gccgtcatgg ccggatgccg cccggaatac
     2581 atgccggtca tcatcgccgc ggtcgagggc atgctcgatc cgagctaccc cctggagttc
     2641 atgcaggtca cgaccaatcc catgacgccg ttcctgctgg tgaacggccc cgtgcggaag
     2701 cagctcgaca tcaattgcgg caccggctgc ctcggcccgg gatggcgcgc caacgcgacc
     2761 atcggccgcg cgatccgcct gatcatgaac aacatcggcg gcgccctgcc cggcgtctat
     2821 tccaaggtga gcttcggctc gccgctgcgc tattcctaca tctgcggcga gaacgaggag
     2881 gagaacccct ggacgccgtt ccacgtggat cgcggcttca ggcgcgaaca gagcaccgtc
     2941 acggtcttca aggcctccaa cttctgcaac atctcgggcg gcgagggcgt cggcccggcg
     3001 gaaatcctgc gccagatcgc aaccaacatg ccgcccatgt acggcggcgg cgacggcgtc
     3061 ctgctgctcc tcggggtgaa ccacgcgctc gcgctccacg aggcgggcct cgccaagcgc
     3121 gacatccaga gccgcctgtg ggaaatggcg cggcttcccg ccagctactt tgccgaggac
     3181 ttcgccgcga ccgagcgcgc ggcgggccgc ggcgacgccg aaacggtgtg gcgcgcccgc
     3241 aatcccgacg agatctacat agccgtcgcc ggcggtccag ggccgcagga cgtctacatc
     3301 ggcgcgggca tgccacagac ccgcgtcatc cgcggcgcct gaggaaacag cttcgatccg
     3361 gcgcgtgcgc cactcccgcc tggtcttcgt tctttgcggc ctcgccttca tcgcggcggg
     3421 gctctttccc atgatcatgg cgctcggcat cgttcccgaa tgggaggaaa aaatgaatgc
     3481 cccacgttgg gtcgtattcc tcgccggggg cctgttcttc atggccggca tgtggctgct
     3541 cctgcaggcc atcgtcggcg aagccgtggc aacggcgttc ggaaccgttg tggggcttgc
     3601 attctttatc ggcctcgccg cggttgggca ctggatggca ttcggagggg gcgaccgcag
     3661 cgattgcagc ggcggcatct cggcgctggg attcgggttc tcgaccagca cgcctgattt
     3721 cgagtgccgc gccgcgttcg gctacggcgc gctgctcatg gacttcatcc tcctgcgcgg
     3781 aatcgcctgg tggatcgccc aacgcatccc cgaaagccgg gccgtgcacg ccttcgagaa
     3841 agctgcggaa tggggcatcg ggctcctcct gctgccgctc gtgctgctcg ccctgctgct
     3901 ctccaagggc aaggacggcg ttacaacgct ggtcggcaag ctgcggtcga accggaatag
     3961 gcaggattaa cctgatttag tcggggctcc ggtcttgtta ataccgtaaa tcctgtccat
     4021 ttcctcttcc taagacaggc tctatactgg tagcccctgc cctgtaccgg gccgaccttt
     4081 caggaggaag ggcctatgta caagaacatc ctgctgccga ccgacggttc ggcgctctcc
     4141 gacgtggcga tcgacaacgg actcaagttc gcgaaggcga tcggcgcgaa agtgaccggc
     4201 ttctacgtga tgatcgagca gcagccggag tcgttcgagg actacgcgcc ggtgcaagtg
     4261 aaggcgccca agctcgagga agtgacgaaa caggaggcgg acaagtatct caaggtgatc
     4321 gccgacaagg cgcgtgcgat gggcgtgccg tgcgataccc gctcgatgaa gcacgcctcg
     4381 ccgcaccagg cgatcatcac gatggcgacg cagaccggct gtgacctcat cgtcatggcc
     4441 tcgcacggca gcaagggcat caccggcgag ctggtcggct ccgaaacctc gcgggtgatc
     4501 acgaactgca agatcccggt gctcgtctac cgctgaaatc cgtgggctga ttcgccgcgg
     4561 tccgaccgga ttcaccgaat cggccgaatc attcccctat aattcacctt ccgcggttcg
     4621 ccccgcggtc cataaacgta gg
//
LOCUS       DBNGWZ010000607        11054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2112554, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000607 DBNGWZ010000000
VERSION     DBNGWZ010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2112554"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="AC16CX_18805"
     CDS             complement(<1..1481)
                     /locus_tag="AC16CX_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HIR3014593.1"
                     /translation="MNDNLLARLRNFFLNLRNRRSTYTLLAAVAIGLTGAPATGIAQP
                     ADKFPNVVCLGCHGNEGFGVPGPDGRMRSLHVIKDKFEKSVHGKRLCVECHKDITEIP
                     HQKVVHRVSCVNCHEALWAAAQKEGKTKEFARLGVVVAQIERYMKSIHARPSRADQSY
                     TNATCYDCHDAHYVYPPGSKARAEWHLNIPNVCGKCHGEQRVEYLDSVHGRLVMGAGN
                     LAAPVCSDCHTTHDVDRPKLAAIKLVITKNCGNCHSENLRSYANTYHGQVSTLGYTYT
                     ATCFDCHGNHGIQRVSHPRSTVHPNNRLQTCQKCHAGATAGFVAFQPHANTHDYGRYP
                     YMWIASKFMIALIAGVFLFFWTHCALWFYREYQERKEGKARPHVLTAEMPQLKDKYYR
                     RFKPVWTVAHLFFALSLMVLSLTGMTAFYAETAWAQSIAKTLGGPRVASVIHRGAAVT
                     ILGIFLAQLVYFAIVLAPKWRTFKWFGHTSFVPSWQDFKDIYA"
     gene            complement(1616..2758)
                     /locus_tag="AC16CX_18810"
     CDS             complement(1616..2758)
                     /locus_tag="AC16CX_18810"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014594.1"
                     /translation="MKKGLILGIVLSALLMGCTEQEKPAVKATTAKPVGDAMAGKAIA
                     GRDCKACHGLDGKGVAPGIPHLAAQRERYLLLALTEYKEVKRTHAALRDLAGRLSGAE
                     LRNVVAYYASQAPVPAASEKGAQQASLLEKGKALAAACVQCHGEEGNSTTPGIPSLAG
                     QQPHYLVTAIQEYHRGERRTAPMKSMLRDASRLELESLALYFASRTPAQRPAPPFGDP
                     QAGEPRTAMCGGCHGPNGVSSDAATPALAGQDPRYLVKSIKAYRTSRQHWGMQRYVAG
                     LSDKDIENISAFYAVQKSRPADSVPSSARELAEKCDRCHDTEDTAKMVVPILKGQDKD
                     YLVMALRAYRDDKRESTTMHRMSVIYSNAIIDDIASYYASQPRDKR"
     gene            complement(2890..3672)
                     /locus_tag="AC16CX_18815"
     CDS             complement(2890..3672)
                     /locus_tag="AC16CX_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein S100"
                     /protein_id="HIR3014595.1"
                     /translation="MSEMPLHRLYHHYSEIPWSQRVLFTAVLIILGFGYLFALIYLFH
                     TYAGKDGNPMTLSYEDLVIAYSGSGKHSRLESALRGPMSTMLPPDEITTFISWVQEGA
                     DRARYEKQIRPAIDKHCMTCHDGSNPHLPNLNGFDNLKKVTERDTGADIFTLVRVSHI
                     HLFGLSFIFFIVGFIFSHAYVRPVWFKCVVVVLPFVAIAVDVSSWYFTKLYHPFAWVV
                     MAAGAVMGLCFAFMWVVSMYQMWFSRTPAAVVQRPGGDTSVG"
     gene            complement(3794..4102)
                     /locus_tag="AC16CX_18820"
     CDS             complement(3794..4102)
                     /locus_tag="AC16CX_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014596.1"
                     /translation="MKRTLLTAGIVALLGVFGAAQAGGDAATGKAKAATCAGCHGQNG
                     EGIKPNPALAGKAEGQFIQAIKDYKSGKRPNPIMKSFADQLSDQDVANLAAYYASLKK
                     "
     gene            complement(4302..5537)
                     /locus_tag="AC16CX_18825"
     CDS             complement(4302..5537)
                     /locus_tag="AC16CX_18825"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014597.1"
                     /translation="MNRSAHVGVLAMVALVAGVTIVGPAPSAAAQPPRVKPGSGDELR
                     AAYATAVEVAEGKRVAEASCARCHGVNGISTTKDVPHLAGQRPAYLYLELHAYQSGAR
                     GDKAMDGAVRFLSDDALIKVAAYYASLEPAQPSVTTAAKGSAKPDPVAAGRAAAAGCA
                     GCHGEGGISKTPGMPSLVALDPKYLVAAMNAYKSGQRKNDMMKSLLAALSDADLNNIA
                     LYFALQKPARAQTPVSGGNPVAGKAAAAVCGACHGDLGVSGSPTTPSLAGQDSQYFAA
                     ALRAYSDGSRKDETMKGPAAALDERVIKDLAAFYAGQQPKPPKVRKPLTTAEWATRCD
                     RCHGVNGNSTDPRLPALAAQRVDYLEKVLHAYRTGARKSPAMAAMSEVLTEADVENLA
                     AYYARQNARAIVYVILPSK"
     gene            complement(5556..6761)
                     /gene="nrfD"
                     /locus_tag="AC16CX_18830"
     CDS             complement(5556..6761)
                     /gene="nrfD"
                     /locus_tag="AC16CX_18830"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR3014598.1"
                     /translation="MIEHVAEIAPFTGYVYPNETVVPWTVLIVVYPYLTGLVAGAFTV
                     SSLYQVFGMQRFKPVAHFALLTSLCLMLFVPAPLLLHLGHPERAFNAVITPHATSAFA
                     VFGYAAGFYVILLLLESWFVFRPHVVRQAQQEKGLLGWFYRVLSLGSYDLSEKAMRYD
                     QKWIFALAVIGIPAAHGLHGYVGFVFGSLKSREWWSSDLMPAIFLFSAVISGTALLIA
                     LYVLSCRLRKVPVDLECVKGLAYTLWGFLMFTLVLEGVEFASLVYRGREGIDVIMEYV
                     AGPLLVPFFILQFGIGSLIPLALLTFMIWQRTTGRALVAGVTASAVLVLLAVLMMRWN
                     VVIGGQEISKTGKGLLSYHPLIFGREGFLAAALVLAAPLVFLLAMVRLFPPWEHNARA
                     SVSSHASVR"
     gene            complement(6758..7606)
                     /locus_tag="AC16CX_18835"
     CDS             complement(6758..7606)
                     /locus_tag="AC16CX_18835"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3014599.1"
                     /translation="MKQETISSLVARRAFLRKIGRAATCAGAIAAAGALGVMQKATAK
                     SEPAPSRGSFPTPDYDWSKRRWGFGVDATKCIGCLRCVEACKRENNVPADAHHFNTWV
                     ERYVYLEGETKARIDSQSDPRNIAASGSEKEYRFDNRYQDAKVEKAFFVPKLCNQCEH
                     PPCVQVCPTGATYKTKDGVVLIDPKYCIGCQYCVQACPYGARYFNVRTGVTQKCYWCY
                     HRITKGLQPACVEACPVGARVFGDRNDPQSPISLFIRNNRVQVLRPETGNGPNVFYVG
                     IDKEVG"
     gene            complement(7603..8601)
                     /locus_tag="AC16CX_18840"
     CDS             complement(7603..8601)
                     /locus_tag="AC16CX_18840"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014600.1"
                     /translation="MPKHIVRLVLLIVAGAAAGLAAKWYFTAESFYQYGHYRANSVAE
                     IAAQEPNHQTPRYCQTCHGERHAQWSANNHKTVSCEVCHGVAKGHPQKAKLTIPTDTR
                     KLCTLCHEAMPGRPRTQPQVALAQHSGGQQCVVCHNPHAPKLAGVVAKVAGDAAAGSK
                     KHAASCASCHGAVGISPNDTWPNLAGQNAAYLVRILGAYKSGDQTDVMMSPMAKTLSD
                     ADIQNLAAYYAGLSCKAAPSEGRVGDTAAGKALAKNCAACHGETGVGSNPAWPKLAGQ
                     KAGYLVNALKAFRAGLRKDPMMAGVTRGLSDADIANLAAYYATQSCEPVTQARKTQ"
     gene            complement(8976..10049)
                     /locus_tag="AC16CX_18845"
     CDS             complement(8976..10049)
                     /locus_tag="AC16CX_18845"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014601.1"
                     /translation="MGNPAHAEDCPFFAKMPKLLTTAIVFSLAVSIAPQAASAQTRER
                     SGKEVVNAVCATCHRTGVKGAPKIGDKKAWSRLASRGLTSLTESALKGVREMPAHGGD
                     LALSDIEIERAITYMVNQSGGKWTEPVGGLTPAVERRGEQIVQAQCAKCHQTGVNGAP
                     KIGDRDAWTPRLKRGLDLLVRSAINGHGPMPPRGGLADLTDSELRGAIVFMFNPSPPP
                     AKGPSTALPKVSDPNHKVVGETEVYLGIVSAESIRAQYSKGSPESSMHGGIPGGKGYY
                     HLNISLFDSKTRAAITDAQVEASVREPTAAETKKLELVTFNNVKSYGNYFRMSSKNPY
                     TITVRVRRPGTSRPVEAKFDYKH"
     gene            complement(10236..>11054)
                     /locus_tag="AC16CX_18850"
     CDS             complement(10236..>11054)
                     /locus_tag="AC16CX_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HIR3014602.1"
                     /translation="YEDRFSIMIDDGGVPGFANQGCWLTCHNGERDNPSFAGRSQVQA
                     NPLYQAIKQNDVRKYLPSTRTDELASWDKGKSLDEIAKIKADGGFLDLMQWRAHRSNP
                     VGMADDGYVLEFRNFDAGGNPFGSNVDPKTHQPRFMYDEKKVGKKALAAGDIRKTSTV
                     LIRGENAVPFDPNAGWKEGDLIPEYVLNKEAKGSAADNKNVKGTWANGTWTVVWVRPL
                     NLANPDDKALREGKVYNFAFAVHDDNITTRGHQVSFPITVGFGAKAAIEATKLK"
BASE COUNT         1981 a         3458 c         3422 g         2193 t
ORIGIN      
        1 atggcgtaga tgtccttgaa atcctgccag ctcggcacga acgaggtgtg cccgaaccac
       61 ttgaaggtcc gccacttcgg cgcgagcacg atggcgaagt agacgagctg cgcgaggaag
      121 attcccaata tggtcaccgc cgcgccccga tgtatgacag acgccacgcg ggggccgccg
      181 agcgtctttg cgatggattg cgcccacgca gtctcggcgt agaacgccgt catgccggtc
      241 agcgacaaga ccatcaatga cagcgcgaaa aagaggtggg ccaccgtcca gaccggcttg
      301 aagcgacggt agtacttgtc cttcagttgc ggcatttcgg ccgtcagcac gtgcggccgg
      361 gccttgccct ccttgcgctc ctggtattcg cggtagaacc acagggcgca gtgcgtccag
      421 aagaagagga acacgcccgc gatcagcgcg atcatgaatt tcgacgcgat ccacatgtag
      481 ggatagcgtc cgtaatcgtg ggtgttggcg tggggctgga acgcgacgaa gcccgcggtc
      541 gcgccggcat ggcatttctg gcaggtttgc agccggttgt tcggatgcac cgtggatctc
      601 ggatggctca cccgctggat gccgtggttg ccatggcagt cgaagcaggt cgcggtatag
      661 gtatagccca gagtggagac ctgcccatgg taggtattcg cgtaggatct cagattctcc
      721 gagtgacagt ttccgcaatt cttggtgatg acaagcttga tcgcggcgag cttcggccgg
      781 tccacgtcat gcgtggtatg gcagtccgag cagaccggag cggcgagatt gccggccccc
      841 atgacgagcc tgccgtggac cgagtccagg tactcgacgc gctgctcgcc atggcatttt
      901 ccgcagacgt tcggaatgtt caggtgccat tccgcgcgcg ccttgctgcc cggcgggtaa
      961 acatagtggg cgtcgtgaca gtcgtagcag gtcgcgttgg tgtacgactg atcagccctg
     1021 ctcgggcggg cgtggatcga cttcatgtag cgctcgatct gggccaccac cacgcccaac
     1081 cgcgcgaatt ccttcgtctt cccctccttc tgcgcggctg cccacagcgc ctcgtgacag
     1141 ttcacgcagc tgaccctgtg cacgaccttc tggtgcggga tttcggtgat gtccttgtgg
     1201 cactcgacgc acagccgctt tccatgcacg cttttctcga acttgtcctt gatcacgtgc
     1261 agggaacgca tccgtccatc cgggcccggc actccgaatc cctcgttgcc gtgacagccc
     1321 aggcatacca cgttgggaaa cttgtcggcg ggctgcgcga tcccggttgc cggcgcgccg
     1381 gttagaccaa tagcaacagc ggcgaggagt gtatacgttg aacggcggtt gcgaaggttg
     1441 aggaagaaat tcctaaggcg cgcgaggaga ttatcgttca tattcggtcc gtcctgcccc
     1501 ggaggaatca cccagccgaa tcacacaata ccgtctggtt gttgtcaagc cggtttcatg
     1561 ccggcaatcg ccgcgtcctg cggcgcaaat gcatatcgac ggcggaattt cccgctcagc
     1621 gcttgtcccg cggctgactg gcgtagtacg acgcgatgtc atcgatgatg gcgttgctgt
     1681 agatgacgct catcctgtgc atcgtcgtgc tttctcgctt gtcatcgcgg tacgctctca
     1741 gcgccatgac cagataatcc ttgtcctgcc ctttcaggat cggaaccacc atcttggcag
     1801 tgtcttcggt atcgtggcag cgatcgcatt tctcagcgag ctcccgggcc gagctcggca
     1861 cgctgtcggc gggcctggat ttctggacgg cgtagaacgc gctgatgttc tcgatgtcct
     1921 tgtcgctcag gccggccacg tagcgttgca tgccccaatg ctgtcgtgat gtgcggtacg
     1981 ccttgatcga cttcaccaga taccggggat cctggccggc gagggctggc gtcgcggcgt
     2041 cactgctgac cccattgggg ccgtggcagc cgccgcacat ggcggtccgg ggctcgcctg
     2101 cctgcgggtc cccaaaaggc ggcgcaggcc gttgcgccgg cgtccgtgac gcaaaataca
     2161 gcgccaggct ttccaattcg agcctgcttg cgtcgcgcag catcgacttc atcggcgccg
     2221 tcctgcgctc tccccggtgg tactcctgga tggcggtgac cagatagtgg ggctgctggc
     2281 cggccaggct ggggatgccc ggagtcgtgc tgttgccctc ttcgccgtga cactgcacgc
     2341 aggccgccgc cagcgccttg cccttttcaa gcagcgaagc ctgttgagcg cccttttccg
     2401 aagcggcggg aaccggggcc tggctcgcgt agtacgcgac cacgttgcgc aactccgctc
     2461 cagacaggcg ccccgcaagg tctctgaggg ctgcatgagt ccgtttcact tccttgtatt
     2521 cggtcagcgc cagcagcagg taacgctcgc gctgcgccgc caggtgcgga atacccgggg
     2581 cgacgccctt tccatccagg ccatggcacg ccttgcagtc tcgccccgcg atcgctttgc
     2641 ccgccatcgc gtctcctacg ggcttcgcgg tcgtcgcctt gacggctggt ttttcctgtt
     2701 cggtgcaacc catcagcagc gccgagagca caatgcccag aataagtcct tttttcatgg
     2761 gaaacccgat gagtgatgga tcggccgagg ttggttaggt ccggtcaaat gtcttgtgcg
     2821 gttgcgccaa cggaaaattc cgactgaccg ctttgtcctt cattgcgtgc agcgggcgcg
     2881 ttttccatgt cagccaacgg aggtgtcgcc gcccggtctt tggacgaccg ccgcgggcgt
     2941 ccgggagaac cacatttgat acatggacac cacccacatg aaggcgaaac acaggcccat
     3001 caccgcgccg gcggccatca cgacccaggc gaacggatga tagagcttgg tgaaatacca
     3061 ggagctgacg tccaccgcga tggcgacgaa gggcagcacg acaacgacgc acttgaacca
     3121 caccggtcgc acgtacgcat ggctgaagat gaagcccacg atgaagaaga tgaaggagag
     3181 cccgaagagg tgaatgtgcg acacccggac cagcgtgaaa atatcggcgc cggtgtcgcg
     3241 ctcggttact ttcttgaggt tatcgaatcc gttgaggttc ggcaggtgcg ggttgctgcc
     3301 gtcgtggcaa gtcatgcagt gcttgtcgat cgccggcctg atctgtttct cgtagcgggc
     3361 gcgatcggcg ccttcctgca cccaggagat gaacgtcgtg atctcgtcgg gcggcaacat
     3421 ggtggacatc ggtccgcgca gggccgattc gaggcgtgaa tgctttccgc tgccgctgta
     3481 ggcgatgacc agatcctcgt acgacaaggt catcggattc ccgtctttcc ccgcgtaggt
     3541 gtgaaacaga tagatcaggg cgaacaggta gccaaagccc agtatgatga gcaccgccgt
     3601 aaacagcacg cgctggctcc acgggatctc cgaataatga tgatagagcc ggtgcagggg
     3661 catctcactc atcggcgggt cctctggtct tgcggagcgg aacactcggg cgcttgacac
     3721 cgcatcccgg gtgttccggc tccagagctg gcgccgctgt cgtgcgctcc ggagtatgcg
     3781 cgagcggtga ccactatttc ttcaacgaag cgtaataggc ggccagattc gccacgtcct
     3841 ggtcgctcaa ttggtctgcg aatgacttca tgatcggatt ggggcgcttg cccgacttgt
     3901 agtccttgat ggcctggatg aactggccct ccgccttgcc ggccagagcg ggattcggtt
     3961 taatcccctc gccgttctgc ccatggcagc ctgcgcaagt ggcggccttg gccttcccgg
     4021 tcgcggcatc tccaccggcc tgtgcggcgc cgaaaactcc aagcaatgcg acgattcccg
     4081 cggtcaacag cgtgcgtttc atggtcaagc tcctatgtgg tggttgggta aatgcgaggc
     4141 gcgaaaaccc gcgcggcatt gcgccgggtt ctcgctgcct cgactggcaa aactcctgaa
     4201 tgcactggcc aggcatacgc ccgctctcat cgctcaccgc aatgtcactg cgcggcaggt
     4261 cggctatggg aggcattgtc ggcgtgttca tgtggcgcgc ctcatttgga cggcagaatc
     4321 acgtacacga tggcccgcgc gttctggcgc gcgtaataag ccgcgaggtt ctccacgtcc
     4381 gcctcggtca acacttcgga catggcagcc atcgccgggc tcttgcgcgc gcccgtgcga
     4441 taggcatgca acactttttc caggtagtcc acgcgctgcg ccgcaagcgc cggcaggcgc
     4501 gggtcggtgc tgttgccgtt gacgccgtga cagcggtcac agcgcgtcgc ccactcggcg
     4561 gtcgtcagcg gcttgcgcac cttcggcggc ttcggctgct ggcccgcgta aaacgccgcc
     4621 aggtccttga tgacgcgttc gtcgagcgcc gccgccgggc ctttcatcgt ttcgtccttg
     4681 cgtgagccgt cactgtaggc ccgaagcgcg gccgcgaagt actgcgagtc ctgacccgcg
     4741 aggctcgggg tggtgggact cccgctgacg cccaggtcac cgtggcacgc gccgcagact
     4801 gcagcggcag ctttgccggc caccgggtta ccaccgctta ccggggtctg cgcgcgcgcg
     4861 ggcttctgca gggcaaagta gagcgcgatg ttattgagat ccgcatcact gagagccgcg
     4921 agcagagact tcatcatgtc gttcttgcgc tggccgctct tgtaggcgtt catggccgcg
     4981 accaggtatt tcgggtccaa cgcgacgagg cttggcatgc cgggcgtctt gctgatgccg
     5041 ccttcgccgt gacaccccgc gcatcccgcc gccgcagccc tgccggctgc gaccggatca
     5101 ggcttggcgg agccctttgc tgcggtcgtc acgctgggct gcgcaggctc gaggctggca
     5161 tagtaggcgg ccactttgat cagcgcatca tcgctcagga atcggacggc gccgtccatg
     5221 gctttatcgc cgcgcgcgcc cgactgataa gcgtgcaact cgaggtaaag atacgccggg
     5281 cgctggccgg cgaggtgggg cacgtccttc gtggtgctga taccgttcac gccgtgacac
     5341 cgggcgcacg aggcttccgc gacgcgcttg ccctcggcaa cctccacggc ggtcgcgtac
     5401 gcagctcgca gctcatcacc gctgcccggc ttgaccctgg gaggctgagc cgccgcactc
     5461 ggcgccgggc ccacgatcgt cacgcctgcg acgagcgcga ccatcgccag gacaccaacg
     5521 tgtgcgcttc ggttcatcga ggctcgttcg aacggtcaac gcacgctggc atgagacgag
     5581 accgaggctc tcgcgttgtg ttcccagggc ggaaacaacc ggaccatagc caacaagaaa
     5641 acaagcggcg cagcaagcac cagggcagcg gccaggaaac cctctctgcc gaagatgagc
     5701 ggatgatagc tcaacaagcc tttgcccgtc ttcgagattt cctgtccgcc gatcaccacg
     5761 ttccagcgca tcatcaatac cgcaagcagc accaacacgg cgctcgcagt gactccagcg
     5821 accagcgcgc gtccggtggt gcgctgccag atcatgaacg taagcagggc gagggggatc
     5881 agggatccga tgccgaattg caggatgaag aatggaacga ggagcggacc ggcaacgtat
     5941 tccatgatca cgtcgattcc ctcccggccc cgatagacca ggctggcaaa ttccaccccc
     6001 tccagtacga gggtgaacat gaggaaaccc cacagcgtat aagccaggcc cttcacgcac
     6061 tccaggtcaa cggggacctt gcgcagcctg caggacaaaa cgtagagggc gatcaacagc
     6121 gccgtaccgg agatgacggc ggaaaacagg aatatagccg gcatgagatc ggaggaccac
     6181 cattcccggg acttgaggga gccaaacaca aatcccacgt agccatgcaa gccgtgagcg
     6241 gcgggtatcc cgatgacggc caacgcgaag atccatttct ggtcgtagcg catcgccttc
     6301 tcggacagat cgtacgaccc gagggacagc acacggtaga accaccccag caggcctttc
     6361 tcctgctgcg cctgccgcac gacgtgaggc cggaacacga accagctttc cagcagcagc
     6421 aggatgacgt aaaaaccggc cgcgtaccca aagacggcaa aggcggacgt cgcatgcggc
     6481 gtaatcacgg cgttgaatgc gcgctccgga tggccgaggt gcaacagcaa tggcgcgggt
     6541 acgaagagca tcaagcacag agaggtgagc aaagcgaagt gcgccacggg cttgaagcgc
     6601 tgcatgccaa acacctgata caggctcgaa acggtgaagg cgccggcaac cagcccggtg
     6661 agatagggat agaccacgat gagcaccgtc cagggaacga cggtctcgtt ggggtagacg
     6721 tagcccgtga aaggggcgat ttcagcgacg tgctcgatca tcccacctcc ttatcgatgc
     6781 cgacgtagaa cacattcggt ccattgccgg tctcgggccg gagcacctgc acgcggttgt
     6841 tgcggatgaa caggctgatc gggctctgcg gatcgttgcg atcgccgaaa acgcgcgcgc
     6901 cgaccgggca cgcttcgacg caggcgggct gcagtccttt ggtaatgcgg tgatagcacc
     6961 aatagcactt ttgcgtaacc cctgtgcgca cgttgaaata gcgcgcgccg taaggacagg
     7021 cctgcacgca gtactggcag ccgatgcaat acttcgggtc gatgagcacc acgccgtcct
     7081 tggtcttgta agtggcgccc gtcggacaga cctggacgca cggcgggtgc tcgcattggt
     7141 tgcacagttt cggcacgaag aacgcctttt cgactttcgc gtcttggtag cggttgtcga
     7201 agcggtactc tttttccgat ccggaggcgg cgatgttccg cggatcggac tggctgtcga
     7261 tcctcgcttt cgtctcccct tccaggtaga cgtaccgctc cacccaggtg ttgaagtgat
     7321 gagcatcggc gggaacgttg ttttcccgct tgcaagcctc cacgcaacgc aggcagccga
     7381 tgcatttcgt ggcgtcgacg ccgaaccccc agcgccgctt gctccagtcg taatccggag
     7441 tgggaaacga gccgcggctc ggggcgggct cgctctttgc cgtggctttc tgcatgacgc
     7501 cgagggcgcc ggcggccgca atggcgcctg cacacgtcgc ggcacggccg attttgcgca
     7561 gaaatgcgcg ccgcgccacc agtgaagata tggtttcttg tttcattgtg tcttcctcgc
     7621 ctgcgtaacg ggctcacagc tttgcgtggc gtagtaggcg gcaaggttgg cgatgtccgc
     7681 atcgctcaag ccccgcgtaa cgcccgccat catcgggtct ttacgaaggc cggccctgaa
     7741 ggcctttaac gcgttcacga gataaccggc tttttgaccg gcaagcttcg gccacgccgg
     7801 attgcttccg acaccggttt cgccatggca cgctgcgcaa ttcttggcca aagcctttcc
     7861 ggccgcggtg tcgccgacac gtccctcgct cggcgccgct ttacagctca ggccggcgta
     7921 ataggccgcg agattctgaa tgtccgcatc gctgagcgtc ttcgccatgg gggacatcat
     7981 gacgtccgtc tggtcaccgg acttgtacgc gcccagtatc cggacgagat aggcggcgtt
     8041 ctgcccggct agattcggcc acgtgtcgtt cgggctgatc ccgaccgcgc cgtggcagct
     8101 cgcgcaactc gccgcgtgct tcttgcttcc cgccgcggca tcgcctgcga ccttggccac
     8161 gacgcccgca agcttgggcg catgggggtt gtgacagaca acgcactgct ggccgcccga
     8221 gtgttgggcg agagcgacct ggggctgcgt gcggggcctc ccgggcatcg cctcatgaca
     8281 cagcgtgcac agttttctcg tatcggtggg aatcgtcagt tttgcttttt gcggatgccc
     8341 cttggccacc ccgtgacaca cctcgcaact gaccgtcttg tggttgttgg ccgaccactg
     8401 cgcgtggcgc tcgccatgac aggtctgaca ataacgcggc gtctgatggt tgggctcctg
     8461 ggcggcgatt tcggcgactg aattggcgcg gtagtgcccg tattgataaa acgactcggc
     8521 ggtgaaatac catttcgcag ccaatccggc cgccgcgccg gcgacaatca acaggaccaa
     8581 ccgaacgata tgcttgggca cgatttaccg tcttgcgctc agcaataatt catcgtcgaa
     8641 tcgagcatag gtgcgtcggc tggttccatg ttgatatgga tcaaaatgcc tcccgcttgg
     8701 cgccgtaaac accggcgccg gccctgccaa agttggagat tatcggcagg caagcactgc
     8761 cctattgata cagatcaaac tccgacatcg tgagcgtgga gggcggaaga ggaacggcgg
     8821 gtatggcgcg cggtgcagtg cccgccgcgc gagcggatcg tgccgcggcg ccggtacggg
     8881 aattggagct gccttctgtc aacgcgggaa ggcggaagag aagaacgaca ccatccctcc
     8941 ttcataggag gggagaatta ttttgtcagg tgctcttaat gcttgtaatc gaacttggcc
     9001 tcgaccggcc gggaagtgcc cggcctccgc acccgcaccg tgatcgtgta cgggttcttg
     9061 ctggacatcc ggaaatagtt gccatagctt ttcacgttgt tgaacgtaac gagctcgagc
     9121 ttcttcgttt ccgctgcagt aggttccctg acgctcgctt ccacctgcgc atccgtgatc
     9181 gcggctctcg tcttgctgtc gaacaacgag atgttcagat ggtaatagcc cttgccgccc
     9241 ggaatgcctc cgtgcatcga gctttccggg cttcctttcg aatactgcgc gcgaatcgat
     9301 tccgccgaga cgatgcccag atagacctcc gtttcaccga cgaccttatg gttggggtcg
     9361 gacaccttcg gcagcgcggt cgaaggcccc ttcgccggag gtggactcgg gttgaacata
     9421 aagacgatcg cgccgcgaag ttcggaatcg gtgagatccg ccaaaccgcc gcgggggggc
     9481 atgggcccgt gcccgttgat cgcggaacgc accagcaaat cgagcccgcg cttgagccgc
     9541 ggtgtccagg catcgcggtc gccgatcttg ggggcgccat tcacgcctgt ctggtggcac
     9601 ttggcgcatt gtgcctgcac gatctgctcg cctctgcgct cgacggctgg cgtcaacccg
     9661 ccgacgggct cggtccactt gccgcctgac tggttgacca tgtaggtgat ggcgcgctcg
     9721 atttcgatgt cgctcagagc caaatcaccg ccatgcgccg gcatttcccg gacgcccttg
     9781 agcgcgcttt ccgtcaggct ggtgaggccc cgcgatgcga gcctggacca cgccttcttg
     9841 tcgccgattt ttggcgcgcc cttcacgccc gtccggtggc aggtggcgca cacggcattc
     9901 accacttcct tgccgctgcg ctcacgggtc tgggcgcttg ctgcttgcgg cgcgatcgac
     9961 accgcaagag aaaaaacgat cgcggttgtc agtagcttcg gcatcttggc gaagaacgga
    10021 cagtcttccg cgtgcgctgg gttgcccata gacagtatcc tatctggtca tatggacatt
    10081 ctaacagctt gtcgggcttt cgccccgaca agctgctgaa ttgaacgaca ttcccagccc
    10141 aatcaatacc ccgatacagc ctgtcggctc cgcggtaagc tggtgcgatc actcatgctt
    10201 tcacaacgac cattgataac accgagtccg acaggctatt tgagcttggt cgcctcgatc
    10261 gcggccttcg cgccgaatcc caccgtgatc gggaacgaca cctgatggcc gcgcgtcgtg
    10321 atgttgtcgt cgtgcaccgc gaaggcgaag ttgtaaacct tgccttcccg caaggccttg
    10381 tcatcgggat tggcgaggtt gaggggccgt acccagacca cggtccacgt gccgttggcc
    10441 caagtcccct tgacgttctt gttgtcggcg gccgacccct tggcttcctt gttgaggacg
    10501 tactcgggga tgagatcgcc ctccttccat ccggcgttgg gatcgaacgg gaccgcgttc
    10561 tctccgcgga tcaggacggt ggacgtcttg cggatgtcgc cggccgcaag cgccttcttc
    10621 ccgaccttct tctcgtcgta catgaacctc ggctgatggg tcttcggatc cacgtttgaa
    10681 ccgaacgggt tgcctccggc atcgaaattg cggaattcga gcacatagcc gtcgtccgcc
    10741 atgccgaccg ggttcgaacg gtgggcgcgc cactgcatca ggtcgagaaa gcccccgtcc
    10801 gccttgattt tcgcgatctc atccaggctc ttgcccttgt cccacgaagc cagctcgtcg
    10861 gtgcgcgttg aaggcaggta cttgcgcacg tcattctgct tgatcgcctg gtacagcgga
    10921 ttagcctgga cctgggacct gcccgcaaaa ctcgggttgt cgcgctcgcc gttatggcag
    10981 gtcagccagc agccctggtt cgcgaagccc ggcacccctc cgtcgtcgat catgatcgaa
    11041 aagcggtctt cata
//
LOCUS       DBNGWZ010000608         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1857976, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000608 DBNGWZ010000000
VERSION     DBNGWZ010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1857976"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..781)
                     /locus_tag="AC16CX_18855"
     CDS             complement(join(12..454,454..781))
                     /locus_tag="AC16CX_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HIR3014603.1"
                     /translation="MDLTDAQWAVLERLFRPPRRADGRGRPWQDTRAVLNGVFWVLRT
                     GAPWHDLPRRYPPYQTCHRRFQQWQRSGLLTRLLQKLAEDLRDRGKLDLSESFIDASF
                     SSAKKGAPAVGPTRRGKGSKIMAISDGHGLPLAVHVASASPHETRLVVPTLQQRFLAA
                     RPQRMIGDRAYDSDPLDLEIRERFGVELVAPHNCTRSKAATQDRRVLRRYRRRWKIER
                     LFAWLHNYRRVVIRWEFYPENFLGMVQLACAIILLRHL"
     gene            1211..2164
                     /locus_tag="AC16CX_18860"
     CDS             1211..2164
                     /locus_tag="AC16CX_18860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3014604.1"
                     /translation="MYERIRARLASREPILLDGANGTELVCRGVRWRKHGLLTDADAV
                     QKLHQEYLAAGADVIRTNTFQLNPRIYLNVFRNREHMRHIGAPGLENLTPRLLRTSVR
                     VARQARASAGRETQAAIAGVLSPLEHCFRPDLAPPPEQARAEHEALARVFAEEHVDFL
                     LLESMNTIGEARAALAAGRATGLPVWVSFVLGPEGELLSREPLAKAAAEMQRGGAEAV
                     LVNCAPPTDITAAIEKLRKGCSLPIGAFAHVGCFSPPSWKFEFFPQFVDTDQWPPERY
                     VAEAKRWRERGATVLGGCCGTGPAHIAALRGWLSEARSARV"
BASE COUNT          438 a          713 c          695 g          400 t
ORIGIN      
        1 tagaagccat ctcataaatg cctcaggagg atgatggcgc aggccaattg aaccatcccg
       61 aggaagttct ctgggtaaaa ttcccagcga atgacgacgc gccgatagtt atggagccac
      121 gcgaacagac gctcgatttt ccagcgacgc cggtagcggc gcagtacgcg gcggtcctgc
      181 gttgctgctt tacttcgcgt gcagttatgc ggagcgacca actcaacccc gaaccgctcg
      241 cggatttcca agtccagcgg atcactgtcg taagcccggt cgccaatcat tcgttggggc
      301 cttgcggcga ggaagcgctg ctggagggtg gggacgacga gccgcgtttc atgcggcgaa
      361 gcgctggcca cgtgcacggc gagaggaaga ccatggccgt cgctgatcgc catgattttg
      421 ctgcctttgc cgcgtcgagt agggccgaca gcagagcccc ttttttcgcc gagctgaagc
      481 tggcgtcgat gaacgactcg ctcaaatcca gcttcccgcg gtcgcgtaag tcctcggcca
      541 gcttctgcag taaccgcgtc agcagcccag aacgctgcca ctgttggaag cggcgatgac
      601 aggtttggta gggcggatag cgacgcggca gatcgtgcca aggagcgccc gtgcgtaaga
      661 cccagaagac accgttcaac acggctcgcg tgtcttgcca cggccggcct cgcccatcgg
      721 cccggcgcgg aggtcgaaac agtcgctcga gcaccgccca ctgggcgtcg gtcaggtcca
      781 tggactggat ttacgccaat ttgcataacc cacaagcctc gaatggcttg aaagtgttta
      841 tgagataact tccagtcgct ttgcagatct attccaattc tgatttgtgg actgccaacc
      901 gtgggttgcc gactgccaac tatgaactct caacttccgc cgcccactat cttcgcgtgc
      961 ataagtcctc tgcaatgtgc atgtgcgaaa aatgcgtccg gaaacccttt agaatccgca
     1021 cttagcccat tttcggggct taaacctcgt agaatcagca cttgcagaaa aaacataggg
     1081 gggtgggggg tagcggtgcc cgacgagtca cgaatcacag gtcacgaacc acaaatcgcc
     1141 ggttccagat tgaaaggcgc tcagccgttc actatgatgg cgcggcttcg agccatcctg
     1201 gagagcgcaa atgtatgaaa ggattcgcgc gcggctggcg agccgcgaac cgatcctgct
     1261 cgacggcgcc aacggaaccg aactggtgtg ccgcggggta cgctggcgca agcacggcct
     1321 gctgaccgac gccgatgcgg tgcagaaact ccaccaggaa tatctcgccg ccggcgctga
     1381 cgtcattcgc accaacacgt ttcaactcaa cccgcgcatc tacctcaacg tgttccgtaa
     1441 tcgcgagcac atgcggcaca tcggcgcacc cggcctggaa aacctgacgc cgcgcctgct
     1501 gcgcaccagc gttcgggtcg cccgccaggc gcgcgcaagt gccgggcggg agacgcaggc
     1561 ggccatcgcc ggagttcttt cgccgctcga gcactgcttc cggccagacc tcgcaccgcc
     1621 gcccgagcag gcccgcgccg agcacgaggc cctggcgcgc gttttcgctg aagaacacgt
     1681 ggattttctg ctcctggaga gcatgaacac gatcggcgaa gctcgcgccg cgctcgccgc
     1741 aggccgcgcc acgggcctgc cggtctgggt gagctttgtc ctggggcccg aaggggagct
     1801 gctgagccgc gagccgctcg ccaaggccgc ggccgagatg cagcgcggcg gcgccgaagc
     1861 cgtgctggtg aattgcgcgc cgcctaccga catcaccgcc gcgatcgaga agctgcgcaa
     1921 gggctgttcg ctgcccatcg gcgcgtttgc gcatgtggga tgcttctcgc ctcccagttg
     1981 gaagttcgag tttttcccgc aatttgtgga caccgaccag tggccgccgg aacgctacgt
     2041 agccgaggct aaacgctggc gtgagcgcgg cgctaccgtt ctcggaggct gctgcggcac
     2101 cggcccggcg cacatcgccg cgctgcgcgg ctggctatca gaggcaagga gcgcgcgagt
     2161 atgaacactt catccatcta tccggcgatc cgcgaaaaac tcgcgcgcgg cgagaccgtg
     2221 attctcgacg gcgccatcgg cacgga
//
LOCUS       DBNGWZ010000609         2847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3120456, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000609 DBNGWZ010000000
VERSION     DBNGWZ010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3120456"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1019)
                     /locus_tag="AC16CX_18865"
     CDS             complement(186..1019)
                     /locus_tag="AC16CX_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3014605.1"
                     /translation="MKSLSTEQRSRQKSRAAGKRTRLSSVSNAMRLLKVFSDDDFEIG
                     VSDLAKRLGLAKSTVHRLASTLVRERILERSARDGKYRLGLLLFELGSLVRRKMDVSA
                     EARPHLKTLMEKTGETVHLGVLDDHSVIYINRIESRRAIRMGFGLGIRAPLHCTALGK
                     ALLAFQPEAFIEETIGQGLPRRTPATITNPQAFRQELAAVRARGYAVEDEEIEAGLRS
                     IATAVRNDSGNVIASIGIAGPAHRLTKKVLLSYARDLMDAAGAVSQRLGYSSPHNQKR
                     A"
     gene            1188..2645
                     /locus_tag="AC16CX_18870"
     CDS             1188..2645
                     /locus_tag="AC16CX_18870"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3014606.1"
                     /translation="MATGNGATFQNDLREFITAVEKRGELARVKNAHWDRELGAVTEV
                     LYRQKVEKSPMLLFDEIPDHPRGYRCAYGMFGSPYRLALVLGMEPALSDNRKEMLDHF
                     RKNIKKGFKRVAPRIVKDGPVLENVLRDGKVDMLKFPVPIHHEDDGGRYIGTACGVVT
                     RDPDSGRINVGTYRVQVKGPNACASYISNGKQGRIHRDKYLKAGKPCPVVIIVGTDPI
                     TYLAAGYTMPDSMPEYEWAGGLMDRPLELIEGETTGLPFPARSEIVMEGEISATETTL
                     EGPFGEWHGYYAGDAQQEPLIHIKRIYHRNDPILTCAASQKPPHSHLFERCFLRSAAL
                     LDALEGAGIPDVKGAWLHQAGAGRTFCVVSVKQRYFGHSTQVGLVASQVASVGYVSRW
                     IVVVDDDVDPTDIHDVIWAMGTRCDPKTRTTLLDHCWSSRLDTLVTDKSLPYNSRMVI
                     DACRPYEQLDTFPKVCQSSPELAAKVRAKYPELYR"
BASE COUNT          495 a          957 c          899 g          496 t
ORIGIN      
        1 ggtgagcgtt ccttcctgct tcgccttctc gatgagccgc ttttcgcggt ccggcccctg
       61 atagttgagg atctcgtcgg ccttcggcgc gccctgggcc agggcaatac ccggctgcag
      121 gccggcaagg actgcgaaaa acacgatgga tcttgcaagc attcgcacga tggcctcctc
      181 gaaactcacg cccgtttctg gttgtgcggg gatgaatagc cgagccgctg ggaaacggcg
      241 cccgccgcgt ccatcaggtc acgcgcgtag gatagcagca ctttcttcgt caggcgatgc
      301 gcgggcccgg cgatgccgat ggaagcgatc acgttcccgg agtcgttgcg gaccgccgtc
      361 gcgatgctgc gcaggccggc ctcgatttcc tcgtcctcga ccgcgtagcc gcgcgcacgt
      421 accgccgcca gctcctggcg gaacgcctgc gggttggtga tcgtggccgg cgtgcggcgg
      481 ggcagccctt gcccgatggt ttcctcgatg aacgcctccg gctggaacgc gagcagcgcc
      541 ttgccgaggg ccgtgcaatg cagcggcgcg cggatgccga ggccgaagcc catgcggatg
      601 gcgcgccggc tctcgatgcg gttgatgtag atgacggaat gatcgtccag cacgccgagg
      661 tggaccgtct cgccggtttt ctccatcaac gtcttcagat ggggccgggc ctccgcgctg
      721 acgtccatct tgcgccgcac cagcgatccc agctcgaaca gcaggagccc gagccggtat
      781 ttcccgtcac gcgcgctccg ctccaggatc cgctccctga ccagcgtcga ggcgaggcgg
      841 tggaccgtgc tcttggcgag ccccaggcgt ttcgccaggt cgctcacgcc gatctcgaaa
      901 tcgtcgtcgg agaagacctt gagcagccgc atcgcgttgg ataccgaaga cagccgcgtt
      961 ctcttgccgg ccgccctcga tttctgccgg ctgcgctgct cggttgacaa tgatttcatc
     1021 ctcgaaaacc cgcgtcgtcc gtgaatattt tttccgcgcg caccggtgcg cggaaaaatt
     1081 gacggtgggg atattgtatg gtcaactggt agcgctctgc aacgagtcgg aggaactgcg
     1141 ttccatatgg aagaacgaac ggtcctgctc tgacgaagga ggcttccatg gcgaccggca
     1201 atggcgcgac attccagaac gacctgcgcg aattcatcac cgccgtcgag aaacgcggcg
     1261 aactcgcccg cgtgaagaac gcgcactggg accgggagct cggcgcggtg accgaggtgc
     1321 tctaccgcca gaaagtcgag aaatcgccga tgctgctgtt cgacgagatt cccgaccacc
     1381 ccaggggcta ccgctgcgcc tacggcatgt tcggctcgcc gtaccgcctc gcgctcgtgc
     1441 tcggcatgga gccggcgctc tccgacaacc gcaaggagat gctcgaccat ttccgcaaga
     1501 acatcaagaa gggcttcaag cgggtggcgc cccggatcgt gaaggacggg ccggtgctcg
     1561 agaacgtgct gcgcgacggc aaggtcgaca tgctcaagtt cccggtgccg atccaccacg
     1621 aggacgacgg cggccgctac atcggcaccg cctgcggcgt cgtcacccgc gacccggaca
     1681 gcgggcgcat caacgtgggc acctaccgtg tgcaggtgaa gggtccaaac gcctgcgctt
     1741 cctacatctc gaacggcaag cagggccgca tccaccgcga caagtacctc aaggccggca
     1801 agccctgccc cgtcgtcatc atcgtcggca ccgatcccat cacctatctc gccgccggct
     1861 acaccatgcc cgactcgatg ccggaatacg aatgggcggg gggcctgatg gaccgcccgc
     1921 tggagctgat cgagggcgag acgacgggcc tgccgttccc ggcgcgctcc gagatcgtga
     1981 tggaaggcga gatctccgcc accgagacca cgctcgaagg gccgttcggc gaatggcacg
     2041 ggtattacgc cggggacgcg cagcaggagc cgctgatcca catcaagcgc atctaccacc
     2101 gcaacgaccc gatcctcacc tgcgcggcga gccagaagcc gccgcactcg catctcttcg
     2161 agcgctgctt cctgcgctcg gcggcgctgc tcgatgcgct cgagggcgcc ggcatcccgg
     2221 acgtcaaggg cgcctggctg caccaggccg gcgcggggcg cacgttctgc gtggtctcgg
     2281 tcaagcagcg ctatttcggc cattcgaccc aggtcggact ggtcgcatcc caggtcgcct
     2341 cggtcggtta cgtcagccgc tggatcgtgg tggtggacga cgacgtggat ccgaccgaca
     2401 ttcacgacgt gatctgggcg atgggcacgc gctgcgatcc gaaaacgcgc acgactctcc
     2461 tcgatcactg ctggtcgtcg cggctcgaca ccctggtcac cgacaagtcg ctgccctaca
     2521 actcgcgcat ggtgatcgac gcttgccgcc cttacgagca gctcgacacc ttccccaagg
     2581 tgtgccagag ctctccggag ctggcggcaa aagtccgcgc caaatatccc gagctctacc
     2641 gctgacttat atggccgccg atgaacgcag atgaacgcgg atagaacccg gagcagccca
     2701 ctgggtcaat tctgctttgt gtcggcgttt atcggcgttt atctgcggct tcatcggttt
     2761 agttccgcaa caatcgcctc ggtcatgcgc cgcgtgccgg cgtcgccgcc gagatcgcgc
     2821 gtgacgaaac gttgctcccg cagcacc
//
LOCUS       DBNGWZ010000610         4715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_472624, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000610 DBNGWZ010000000
VERSION     DBNGWZ010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_472624"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16CX_18875"
     CDS             complement(<1..773)
                     /locus_tag="AC16CX_18875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3014607.1"
                     /translation="MHTNAVESLLPIKIGKARIPYARGVRAGPWVFASGILATDDKGG
                     LAPEARDGGRPLSGLPRWYREALCLYRRAEEVLKAGGTDLAHAVRTDQYFPDWRAVPF
                     LHLARKERCGDYIAPSTSVLEPRLLLPGAGMAMEMIAVRPGAGLDVKPLYPPGLEVPA
                     TSAFAPVATAGDYVFVAGFLAAWKPGDLGGIAPEAKVPEGHLWKGNRIQLEADYIIRR
                     KLAPALEGAGASLASVVKAQVYLADIHDVPAFNQVWARH"
     gene            862..1104
                     /locus_tag="AC16CX_18880"
     CDS             862..1104
                     /locus_tag="AC16CX_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014608.1"
                     /translation="MAGMIERASRKESPEELGARLLATCRWCDEMRRLALSGLRSQRP
                     HASARELDDMLYTFRLRWEQVNQRLRRRQAALHGND"
     gene            1097..1660
                     /locus_tag="AC16CX_18885"
     CDS             1097..1660
                     /locus_tag="AC16CX_18885"
                     /inference="COORDINATES: protein motif:HMM:NF039330.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HIR3014609.1"
                     /translation="MTEGGSAPDYVTALSAFARARPKPAPDYCLIGALALGVWGAVRA
                     TQDIDFLVLVEDDRRDALMAALAASGFRVDERWAELNPMAKNSFVRLAFGPHAIDLML
                     ARDAFHREALARRRNVSIEGQTMAVASAEDLMLLKLRAGRDQDFVDVVSVATRQGSAL
                     DLAYLKQWAERLGVSGELEYALKTAAA"
     gene            complement(1702..1932)
                     /locus_tag="AC16CX_18890"
                     /pseudo
     CDS             complement(1702..1932)
                     /locus_tag="AC16CX_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            2163..3194
                     /locus_tag="AC16CX_18895"
     CDS             2163..3194
                     /locus_tag="AC16CX_18895"
                     /inference="COORDINATES: protein motif:HMM:NF048867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR3014610.1"
                     /translation="MTAAVLVATGVLAAGSAFAQGKWTSLKPIPQGEEEVYGTAAGGK
                     LYVLGGLGIFPGWEPKQMLWSFDPATNEWTRLPNIPEGIHHPGFAAVGDKLYSIGGFT
                     IARPATGLPAWVPSKSVWIYDITAKNWSKGPDLPTPRGALTATAHGTRIYAIGGAKNP
                     AYSTPELRPTVPVENMATNEVFDTATGTWSAAAPMLTARNHHGAALIDGKIYVVGGRI
                     GSTFIIGLSNNISTNEVYDIAKNTWAAVLGMPTPRSGIGTAVLNGRMHVLGGEAYLND
                     LVGTYRTHEAFDPKTNTWQRLPPMPTPRHGLAVAEIGGKMYAVSGSNVAGGGGPHEGV
                     KVNEVYDPN"
     gene            complement(3200..>4715)
                     /locus_tag="AC16CX_18900"
     CDS             complement(3200..>4715)
                     /locus_tag="AC16CX_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014611.1"
                     /translation="RSVSRSTPDSEIMPLCWHKPLTSLTLVSGLAAAGPAAAHGFGQR
                     YDLPLPLSFYIWGAGATVALSFIGFALFLRAEHRLPGWHFDRRIEGRLAGALTAVPRA
                     LAAGVLVLVIVAGLFGNQDPVRNIAPVTIWVIGWVGLAFLSVLLGDVWRLLNPWSAVF
                     SLAEACCRRARAGTVLGLARPYPEALAAWPGFVLLVAFAWMELVWGGKNEPAALAAAI
                     AVYSALTFFAMAWFGREKWLANGEVFTLVFGTFARFGPLVGLPQQPGIRLRMPAAAGL
                     LADQPSTFSMVALVVALLATVTFDGLLETPPWARVDLAVLGWASEPPFWAASILREDQ
                     AVRLVRSLALVGCLLLFLAVYAAVCRLTAAAAGGRALRTGVVLRSFVLTLVPIAIAYH
                     VAHYFSFFFLGGQYFIPLLSDPLGRGWNLFGTAGYPIDIGLITPRLQWSVAVVAVVLG
                     HVCAIYLSHVTALRLFTDRRAALRSQVPMVALMVAYTMLSLWILSQPIVETGVR"
BASE COUNT          895 a         1528 c         1596 g          696 t
ORIGIN      
        1 aaatgccgcg cccagacctg gttgaacgcg ggaacgtcgt ggatgtccgc cagatagacc
       61 tgcgccttca cgacgctcgc gagcgaggcg ccggcgcctt cgagcgcggg cgcgagcttc
      121 ctgcggatga tgtagtcggc ctcgagctgg atgcgattgc ccttccacag atggccctcg
      181 ggaaccttcg cttcgggcgc gatgccgccg aggtcgccgg gcttccacgc cgcgaggaat
      241 ccggcgacga atacgtagtc gcccgcggtc gccacaggcg caaaggcgga cgttgcagga
      301 acctccagtc ccggcgggta caacggcttg acatcgagac ccgcgccggg acgaaccgcg
      361 atcatctcca tcgccatgcc ggctccgggg aggagcagcc ggggctcgag caccgaggtg
      421 ctgggagcga tgtaatcgcc gcagcgttcc ttgcgcgcga gatggaggaa gggcaccgcg
      481 cgccagtcgg gaaaatactg gtcggtgcga accgcgtgcg cgaggtccgt gccgcccgcc
      541 ttgagcactt cctcggctcg ccggtagagg cacagcgcct cgcggtacca gcgcggcagg
      601 cccgacagcg ggcggccgcc gtccctcgct tcaggtgcaa ggccgccctt gtcgtcggtc
      661 gcgaggatgc ccgaagcgaa gacccagggc cccgcgcgca cgccccgcgc gtacgggatc
      721 ctcgccttgc cgatcttgat gggaagcagg gattcgacgg cgttggtgtg catgctgggc
      781 gactcggata caaagggagc agtgtaacgt tttcaaggcg cgggtagttg cggttggcgc
      841 ttcaccgtac ggcttgctaa gatggccggc atgatcgagc gcgcgtcccg aaaggaatcc
      901 ccggaggaac tgggcgcccg gctgctggcg acgtgccgct ggtgcgacga gatgcgccgg
      961 ctggcgttgt ccgggctgcg gtcgcaacgg ccgcacgcgt ccgcccggga gctggatgac
     1021 atgctctaca cgttccgtct gcgatgggaa caggtgaacc agcgcctgcg gcgccgtcag
     1081 gctgcattgc acggcaatga ctgagggcgg gagtgcgccg gattacgtca ccgcgctatc
     1141 ggctttcgcg cgcgcgcgtc ccaagccagc tcccgactat tgcctgatcg gagccctggc
     1201 gcttggcgtc tggggcgcgg tcagggcaac gcaggacatc gatttcctcg tgttggtgga
     1261 agacgatcgc cgtgacgccc tgatggcagc attggccgct tcgggattcc gggttgacga
     1321 acggtgggcg gagctcaacc cgatggcgaa gaatagcttc gtgcggctcg ctttcggccc
     1381 gcacgcaata gatctcatgc tggcgcgcga cgccttccac cgggaggcgc tcgcccgcag
     1441 gcgcaacgtc tcgattgaag gccagaccat ggccgtcgcg agcgcggaag acctgatgct
     1501 cctgaagctc cgtgccggcc gcgaccagga cttcgttgat gtcgtcagcg tggcgacgcg
     1561 ccagggatct gcgcttgacc tcgcctatct gaagcagtgg gcggagcggc ttggcgtttc
     1621 gggcgaactc gaatatgcgc tgaaaacggc tgccgcctag gccctttcgc cattcgccat
     1681 caacatttcg agggccgctc cctacctcgg ctgcgacacg accctgaggt acggcttcag
     1741 cgtcttgtag ccctgcggat atttcttccg ggcgtcctcg tcggacaccg acgtcgggat
     1801 gatcacgtcc tcgcccggct tccagttcac cggcgtcgcc accgtgtgtt tcgcggtgag
     1861 ctggagcgag tcgagcaggc gcagcacctc gtcgaaattg cagcccgtgc tcatcggata
     1921 gaccagcatc attgtcttag gggacagacc actaaggaga atttttagat ggatcagctt
     1981 gctattgtcc ttagtggtct gtcccctaag attcgttaca cgcgctcgat gatcgcgcgg
     2041 gcgcgaattg caatttggca ggcgatcctt gatactacgt ccccggctcg gtgcccggca
     2101 cggcggtcgc ggcggccgcg gcacgcgacc gcaaacaggg gagaaactca atgcgtgcag
     2161 caatgacggc ggccgttctg gtcgcaacag gtgtgctggc ggccggttcc gcattcgccc
     2221 aaggcaagtg gacctcgctc aagccgatcc cccaaggcga agaggaagtg tacggaacgg
     2281 cggcgggggg caagctctat gtgctcggcg ggctcggcat attcccgggc tgggagccca
     2341 agcagatgct gtggagcttc gatccggcga ccaacgaatg gacgcgactg cccaacattc
     2401 ccgagggcat tcaccatccc ggtttcgcgg ccgtcggcga caagctctat tcgatcggcg
     2461 ggttcacgat cgcgcggccg gcgaccggat tgccggcgtg ggttccgagc aaatcggttt
     2521 ggatctacga catcacagcc aagaactggt cgaagggacc ggatctaccg accccgcgcg
     2581 gcgcgctgac cgccaccgct cacggaacca ggatctacgc gatcggcgga gccaagaacc
     2641 cggcttattc gaccccggaa ctgcgaccga ccgtgccggt ggagaacatg gcgacgaatg
     2701 aggtgtttga taccgcgacc ggcacctggt cggcggcggc gccgatgctg acggcgcgca
     2761 accatcacgg ggctgcgctg atcgacggca agatctacgt ggtgggaggg cgtatcggat
     2821 cgacctttat catcgggctt tccaacaaca tcagcaccaa cgaggtctac gacatcgcga
     2881 agaacacctg ggcggcggtg ctgggaatgc ccactccgcg cagcggcatc ggaaccgccg
     2941 tgctcaacgg acggatgcac gttctaggag gcgaggccta cctgaacgac ctggtcggca
     3001 cctaccggac gcacgaggcc ttcgatccga agacgaacac gtggcagcgg ctgccaccaa
     3061 tgccgacgcc ccgccacggc ctcgccgtgg ccgagatcgg cggcaagatg tacgcggtca
     3121 gcggatccaa cgtggccggc ggcggcgggc cgcacgaggg agtcaaggtc aacgaggtct
     3181 acgatcccaa ttaggcgctc tagcgaacgc cggtttcgac gatgggctgt gacagaatcc
     3241 acaagctcag catcgtgtag gcgaccatca gtgcgaccat cgggacctgg ctcctcagcg
     3301 ctgcacgccg atcggtgaag agccgaagcg ccgtgacgtg cgagaggtag atcgcgcaca
     3361 catggccgag cacgacggcg accaccgcga cgctccactg cagccgcggc gtgatcagtc
     3421 cgatatcgat cggatacccg gcggttccga acaggttcca gccgcggccc aggggatctg
     3481 acagcagcgg gatgaagtat tgtccgccga ggaaaaaaaa tgagaaataa tgcgccacgt
     3541 gataggcgat cgcgatcggc acgagcgtga gcacgaagct gcgcagcacg acgccggtgc
     3601 gcagcgcgcg cccgcccgcg gcggccgcgg tcaagcggca cacggccgcg tagaccgcga
     3661 gaaacagcaa gaggcatccc acgagcgcca ggctgcgaac cagccgcacg gcctggtctt
     3721 cacgcagaat cgacgcagcc cagaacggcg gctcggaagc ccaacccaga accgcgagat
     3781 cgacccgcgc ccaaggcggg gtttcgagca gcccgtcgaa agtgacggtc gccagcagcg
     3841 cgaccaccag cgcgaccatg gagaacgtcg acggctggtc ggcgagcaat cccgccgccg
     3901 ccggcatgcg caggcggatg cccggctgct gcggtaggcc caccagcggg ccgaaccgcg
     3961 cgaaggtgcc aaagacgagc gtgaacacct ccccgttcgc gagccatttc tcgcgtccga
     4021 accaggccat ggcgaagaac gtcagcgcgg agtagacggc gatcgcagcg gcgagcgcag
     4081 cgggttcgtt cttgccgccc cacacgagtt ccatccaggc aaacgccacg aggagcacga
     4141 agccgggcca cgccgccagc gcttccggat agggccgcgc gaggccgaga acagtgcccg
     4201 cgcgcgcccg gcggcagcag gcctcggcca gcgagaaaac cgcgctccac ggattgagca
     4261 gcctccagac gtcgccgagc agcaccgaca agaacgccag tcccacccag ccgatgaccc
     4321 agatcgtgac cggcgcgata ttacgaaccg gatcctgatt gccgaacagt ccggcgacga
     4381 tcaccagcac cagcacgccg gccgcgagcg cgcgcggcac ggcggtcagc gctcccgcga
     4441 ggcgaccttc gatgcgccga tcgaaatgcc agccgggaag cctgtgctcg gcgcgaagaa
     4501 acaaggcgaa accgatgaac gacaaggcga cggtcgcccc ggcgccccag atgtagaacg
     4561 aaagcgggag cggcaagtcg tagcgctggc cgaaaccgtg cgcggcggcg ggaccggccg
     4621 cggcgagacc ggacacaagc gtcaggcttg tgaggggctt gtgccagcag agcggcatga
     4681 tctcgctatc gggggtggat ctcgagaccg agcgg
//
LOCUS       DBNGWZ010000611         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_225348, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000611 DBNGWZ010000000
VERSION     DBNGWZ010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_225348"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..281
                     /gene="ccmD"
                     /locus_tag="AC16CX_18905"
     CDS             108..281
                     /gene="ccmD"
                     /locus_tag="AC16CX_18905"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR3014612.1"
                     /translation="MSWASWHDFFAMGGYALYVWGSYGVTAGLIVVEIVLLGMRRRAA
                     LEQLGRPAAGDRP"
     gene            278..763
                     /gene="ccmE"
                     /locus_tag="AC16CX_18910"
     CDS             278..763
                     /gene="ccmE"
                     /locus_tag="AC16CX_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR3014613.1"
                     /translation="MKPRHKRVAMLAGGLAALAVAAGLILAAFQKNLVFFFTPSQVAA
                     NEAPQGKTFRIGGLVETGSVKRQPDGLTVRFTVTDTAKSIPVVYRGALPDLFREGKGI
                     VAQGRVGPDGVFQASEVLAKHDENYMPPEAADAVKRAHEVSRKAAQTVVTGSGSGGER
                     R"
     gene            760..>2560
                     /locus_tag="AC16CX_18915"
     CDS             760..>2560
                     /locus_tag="AC16CX_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR3014614.1"
                     /translation="MIPELGQFALVLALCLALVQSVLPIAGAARGDAVWMGVARPAAQ
                     GQFVFVAIAFGCLAWSFASNDFSVLNVATNSNSQLPLHYRLAATWGSHEGSLLLWVLV
                     LTAWMVAVTVFSRHLPDDMVARVLGVMGLISVGFLSFMLFTSNPFDRLLPAAPDGRDL
                     NPLLQDPGMVFHPPLLYMGYVGFSVAFAFAIAALLAGRLDATWARWSRPWTTVAWMFL
                     TLGIALGSFWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGTFRSWTV
                     LLAILAFALSLLGTFMVRSGVLTSVHAFATDPKRGIFILGFLAVVVGGALALFAWRAQ
                     RIGVGGRFDLVSRESLLLTNNVLLLVAAGSVMLGTLYPLTLDALSLGKISVGPPYFET
                     VFVPLLAPALFLMAVGPIARWKKAELPELAVRLRWALGVSIAAAVLVPLAMGRSGALV
                     GFGIGLAVWIVASAVVSLLDRMQPAGAGVTGISARLRATPRAYYGMLLAHIGVAVFVI
                     GVTLVKGYETEKDVRMAPGDTVEVGGYTFRFEGVMDVQGPNYAAARATLSVAREGRAV
                     ATLHPEKRIYRVQETPMTESAIDYGAFRHLYVAL"
BASE COUNT          333 a          849 c          899 g          479 t
ORIGIN      
        1 gcctggatgt acagcatcgc cgcgtcgttg catcgggtgc gttcgatcat gctggagcgc
       61 gagcgcggcg ccgactgggt cgcgcagcac gcgagggcgg tgcggcgatg agctgggcaa
      121 gctggcacga ttttttcgcg atgggcggct acgccctcta cgtgtgggga tcgtacggcg
      181 tcaccgccgg tcttatcgtc gtggaaatcg tcttgctggg aatgagaagg cgcgccgccc
      241 tcgaacagct cggccgcccc gctgcggggg accggccgtg aagccgaggc acaagcgcgt
      301 cgcgatgttg gccggggggc tggccgcgct ggcggtggcg gcggggctga tcctcgccgc
      361 attccagaag aacctcgtgt tcttcttcac cccttcccag gtcgccgcca acgaagcgcc
      421 gcagggcaag actttccgca tcggcgggct ggtggagacg ggaagcgtca agcgccagcc
      481 cgacgggctg acggtgcgct tcaccgtgac cgacaccgcg aagagcattc ccgtggtgta
      541 ccgcggcgcc ctgcccgacc tcttccgcga aggcaaaggc atcgtcgcac agggccgcgt
      601 cgggccggac ggggtattcc aggcaagcga ggtgctcgcc aagcacgacg agaactacat
      661 gccgccggag gccgcggatg cggtaaagcg cgcgcacgag gtgagccgca aggcggcgca
      721 gaccgtggtg accggttccg gcagcggagg ggagcggcga tgattcccga actcggccag
      781 tttgccctgg tgctcgcttt atgcctcgcc ctggtgcaaa gcgtgctgcc gatcgccggc
      841 gcggcgcgcg gcgacgccgt ctggatgggc gttgcgcgcc ctgccgcgca gggacagttc
      901 gtgttcgtcg cgatcgcgtt cggctgcctc gcctggtcgt ttgccagcaa cgatttctcc
      961 gtgttgaacg tcgccaccaa ctccaattcc cagttgccgc tccattaccg ccttgccgcg
     1021 acctggggtt cccacgaggg ctcgctgctg ctgtgggtgc tggtgctgac ggcatggatg
     1081 gtggcggtga cggtgttcag ccgccacctg cccgatgaca tggtggcgcg ggtgctcggc
     1141 gtgatgggct tgatcagcgt cggttttctc tcgttcatgc tgttcacctc caacccattc
     1201 gaccgcctgt tgccggcggc gccggatggc cgtgacttga atccgctgct gcaggacccg
     1261 gggatggtgt tccacccgcc gttgctctac atgggctacg tcgggttctc ggtggcgttc
     1321 gcgttcgcca tcgccgcgct gctcgcgggc cggctcgacg cgacctgggc gcgctggtcg
     1381 cgcccgtgga ccacggtggc ttggatgttt ctcacgctcg gcatcgcgct gggcagcttc
     1441 tgggcctact acgagctcgg ctggggcggc tggtggttct gggacccggt ggagaacgcc
     1501 tccttcatgc cgtggctggt cggcaccgcg ctgatccatt cgctcgcggt caccgagaag
     1561 cgcggcactt tcaggagctg gaccgtgctg ctcgcgatcc tcgcgtttgc actctccctg
     1621 ctcggcacct tcatggtgcg ctcgggtgtg ctcacctcgg tgcacgcgtt cgcgaccgat
     1681 cccaagcgcg gcatcttcat actcggcttc ctcgcggtcg tggtcggagg ggcgctcgcg
     1741 ctgttcgcct ggcgcgcgca acgcatcggc gtcggcggcc gcttcgatct ggtgtcgcgc
     1801 gagtcgctgc tgcttaccaa caatgtgctg ctgctcgtcg ctgccggctc ggtcatgctg
     1861 ggcacactct accccctcac gctcgacgcg ctctcgctcg gcaagatctc ggtgggcccg
     1921 ccctacttcg agacggtgtt cgtgccgctc ctggcgccgg cgctgtttct gatggcggtc
     1981 ggcccgatcg cacgctggaa gaaggcagag ctgcccgaac ttgcagtacg gctgcgctgg
     2041 gcgctcgggg tgagcatcgc agccgcggtc ctggtgccgc tcgcgatggg acgctcgggc
     2101 gcgctggtcg gcttcggcat cgggctcgcg gtatggatcg tcgcttccgc cgtcgtgagc
     2161 cttctcgatc gcatgcagcc cgccggcgcc ggagtcacgg gcatcagcgc ccggctgcgc
     2221 gcaacaccgc gcgcttatta cgggatgctg ctcgcgcaca tcggggtcgc agtgttcgtc
     2281 atcggcgtga ccctggtcaa gggctacgag acggagaagg acgtgcgcat ggcgccgggc
     2341 gacaccgtgg aagtcggcgg ctataccttc cgcttcgagg gcgtcatgga cgtacagggc
     2401 ccgaactatg ccgcagcgcg ggcgaccctt tcggtcgcgc gggagggccg cgccgtcgcg
     2461 acgctccacc cggagaagcg catctaccgc gtgcaggaaa cgccgatgac cgagtccgcg
     2521 atcgactacg gcgctttccg ccacctctac gtcgcgctgg
//
LOCUS       DBNGWZ010000612         8036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_225507, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000612 DBNGWZ010000000
VERSION     DBNGWZ010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_225507"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="AC16CX_18920"
     CDS             <1..828
                     /locus_tag="AC16CX_18920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014615.1"
                     /translation="TIAERLSKNLGQQVIVDNRPGGGTVIGAELAARSAPDGYTLLLG
                     TATTHAINASLVRKLPYDPIRDFAPISLVAVLPQIIVAHPSLPAQTLKELIALARKRP
                     GEINFASTGNGSANHLGGEMLKSVAKVSIVHVPYKGASPAVIDLLAGNVQFMFSTIPP
                     VLPHVKSGRLKALAVAQARRSTLLPELPTTAEEGAPGVEAASWNGVLVPAGTPAEIIA
                     KLHGELAAVMGLPEVRARLAMSGVEPQVNTPAEFAVYIASETARYAKVVKESGAKLD"
     gene            complement(1182..2090)
                     /locus_tag="AC16CX_18925"
     CDS             complement(1182..2090)
                     /locus_tag="AC16CX_18925"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR3014616.1"
                     /translation="MRQPPRRRPVRLVLGFSPGSASDQVARLIAGPLAERIGKPVRIE
                     RRPGRNGAVAASEVAAGAADGHTLFVATLGTHALAPYLDPRLSYDPLVDFAPVSLLSR
                     APMLLACHPAVAAGSARELIELARARPRELAYATSATGGAPHLAAELFQEMAGVEMRH
                     ARYDHTERLYEELESGLVSLSFNNIMSMLPRCRAGTLRPLGISALERSEAAPDLPTIA
                     ESGLPGYEVSNWLGIVAPKATPPATLAELSAAVRDVMRSEAVVRPLHAAGVAPCGSSP
                     EEFRRFMLGEIRRWGPIVARFRHVAA"
     gene            complement(2146..3123)
                     /locus_tag="AC16CX_18930"
     CDS             complement(2146..3123)
                     /locus_tag="AC16CX_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014617.1"
                     /translation="MKVARSAILCAVIVAAIPLASGAAFAQPYPARPLRFVIPFPPGG
                     GADNLARIVGQPAGEKLGQQIVIDNRAGAGGNIAAEVVAKAAPDGHTLLQGNVAHAIS
                     ATLYRKPPYDILKDFTPVTQLASIPFVLAVNPALNANSVKDFIALARAKPGRLNYASS
                     GNGGPSHMAMELFKSMAGIEIRHVPYKGAAPAATDLIAGQVQAGFFTVSAALPYTTSG
                     RLKALAIASSRRTLLAPDIPTVGEAGLPGFEATTWFGVMVPRGTPASVVGKLHAAFVA
                     ALNAPGVRERISNQGFEIVGSSPAEFSSYVRNEIPKWAKVVKSSGASVD"
     gene            complement(3161..4123)
                     /locus_tag="AC16CX_18935"
     CDS             complement(3161..4123)
                     /locus_tag="AC16CX_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HIR3014618.1"
                     /translation="MTLQQLRYLCEVVRQNLNISKAAQAMHVSQPGLSRQLQLLEREL
                     EVDLFVRNRKRLVRLSQAGSQIVSLAERTLNDARSIKEAAKEHSGQDQGTLIIATTHT
                     QARYALPEVIKRFAQRYPKVQLSLRQGTPAEVSYLVSSGSADLSIATEPLDPPADVVL
                     LPCYQLERIVLTPARHPLLKVRRLTLEKLAAYPLITYDYAFIGRSKILRAFEAKGIVP
                     NIVLNAIDADVIKTYVESGLGIAIVPVMAFDRARDKSLRGIDASHLFEPNTIHVGIRR
                     NHYLRGYMYSFIEMFVPHLRRSVVEKAITESATRRAVRAAAPVR"
     gene            4252..5391
                     /locus_tag="AC16CX_18940"
     CDS             4252..5391
                     /locus_tag="AC16CX_18940"
                     /inference="COORDINATES: protein motif:HMM:NF020031.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3014619.1"
                     /translation="MMLLEHEGKRLLGAVGVPVPDGVVVAAQPESLRDAGARISSYPV
                     AVKAQVRGGGRGKRGGVQQVMTAGDLAAAVRKLLDTEFGGEKPEAVLVEPWLAIERET
                     YLSVAVDVRADGYVVMYSPKGGIDIEDGPPPARYPVGVPWNFRVHGLRRVLESVEPDY
                     QWREKVITLAQRLVRLAAMRDCTTIEINPLVRLADGSLIAADAKVACDEWAHFRNREI
                     HARREAEKARANRLLRDCLQMQHMYVRLDGDIALISGGAGMTMAAMDMIADYGGCPAC
                     FLDCSPGPTSTRGYRPAFAMLDADPQVKVILVSVFGGGTQMQRVANSMKEIMAERRSA
                     KPVVFRLDGTNVDQVSGIFAQFGAHNHARLEDAVAEAVRLAAGKK"
     gene            5388..6284
                     /locus_tag="AC16CX_18945"
     CDS             5388..6284
                     /locus_tag="AC16CX_18945"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009361 - succinate-CoA ligase complex
                     (ADP-forming) [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004775 - succinate-CoA ligase
                     (ADP-forming) activity [Evidence IEA];
                     GO_function: GO:0004776 - succinate-CoA ligase
                     (GDP-forming) activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3014620.1"
                     /translation="MSILLDRTNRVLVQGVTGQMGTFFVQDARRYGTTVVAGVSPGRA
                     GTMVGDVPVFGSVREAREATGADTAIVFVPPAAVLGGALEAIEAGCRLVVVTADELPV
                     LDAIELRAAARANGAALVGPNSPGLISPGKAKLGFMPSFCYMQGDVGVISRSGSLSYE
                     CCKRLTLAGMGQSTVVGIGGDAVKGTTAAEALQLFHDDPETSAVLYLGEIGGSEEIFV
                     AEYAIRPDAKPVAALIVGKTAPSGRKMGHAAALIGSKAEGHGAKTALLREAGVRVASG
                     LEQVATVTREALDSRRPAAAAA"
     gene            6325..7527
                     /locus_tag="AC16CX_18950"
     CDS             6325..7527
                     /locus_tag="AC16CX_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAS1-like dehydratase domain-containing protein"
                     /protein_id="HIR3014621.1"
                     /translation="MNKRVDLGITEQAAEGLINDQSLAAARALVGAKLRPEQYLRDAS
                     VDSITIFANGIGDLNPLYRDQEYARWTRFGGIIAHPCFPWTHHWPGRSYWGLPGVHGF
                     GVAIDCEFYRNVRPGDRINIWNRVLGVEEKPSKFSGRMAMQYLESTYTNQRDEILCRA
                     LGLTARHERKASREKGKYKEIKTHVYTPEERARIDDMVLSEPSRIRGVETRYWDDVKV
                     GEKIDEIARGPLSMSDTMAFVIASGRGAAHGALLRHAAKHPKHYIRNKQAGGGIEYTG
                     IGHHREDFAREVGVPGMYDYLPQRACWFATAVTNWMGDDGVLKRLRMEARMFNCQGDT
                     TFICGSVVKKYVKDRCALVDIEMKGVNQRGDLTSPGFATVMLPARDIHTRIPIDGTVV
                     ELDLPPIR"
     gene            7543..>8036
                     /locus_tag="AC16CX_18955"
     CDS             7543..>8036
                     /locus_tag="AC16CX_18955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3014622.1"
                     /translation="MSMATDAMYLVCDMINDLVHEDGPNGKKGYGPELKRRNTIANTA
                     EALRKARAAGVLIGYVRVGFSPDYRECPPTSRMFQGAKKAGMFKLGSWGTEVHPALAP
                     QPGDHDIVKRRVSPFYATSLKAILRANGIRRLYLSGVSTSGAVLSGAKDGHDRDYDVY
                     VIED"
BASE COUNT         1391 a         2510 c         2817 g         1318 t
ORIGIN      
        1 acgattgccg aacggctctc gaagaacctc ggacagcagg tgatcgtcga taaccggccg
       61 gggggtggca cagtgatcgg cgccgaactt gccgcgcggt ccgcgccgga cggttacacg
      121 ctgctactcg gcaccgccac gacgcacgcc atcaacgcga gcctcgtcag gaaactgccg
      181 tacgacccga tcagggattt cgcgccgata tcgctggtgg cggtcctgcc ccagatcatc
      241 gtggcccacc cgtcgctgcc ggcccagacg ctcaaggagc tcatcgcgct cgcgcgcaag
      301 cggcccggcg agatcaattt cgcctcgacg ggcaacggca gcgccaatca cctgggcggc
      361 gagatgttga agagcgtcgc caaagtgagc atcgtgcacg tgccgtacaa aggggcttca
      421 cccgcggtca tcgatctgct ggcgggcaat gtgcagttca tgttctcgac catcccgccg
      481 gtgctgccgc acgtgaaatc ggggcgcctg aaagcgctgg ccgtcgccca ggccaggcgc
      541 tcgacgctgt tgcccgaact gccgaccacg gccgaggagg gcgcacccgg tgtggaggcg
      601 gcctcttgga acggcgtatt ggttcccgca ggcacgccgg ccgagatcat cgcgaaactt
      661 cacggcgaac tggcggcggt catgggtctc ccggaagtgc gcgcgcggct cgccatgagc
      721 ggcgtggaac cgcaggtcaa cacgccagcc gaattcgcgg tgtatatcgc gtccgagacc
      781 gcgcgctacg ccaaagtggt caaagagtcg ggcgccaagc tggactgagg cgccgctcgc
      841 cggagcagag gaggggcggg tgacggcgtt gctgccgtac gatacccttc gcgctccgag
      901 cggctgcgca gctttctcga ccaggaaagc tgaccggaat cgcactcggc tgggtatcgc
      961 cgggcaatcg gcgccgtcgg cgtggcgcac tccgttgccg gtcagcttta acgaaacatt
     1021 ccttttccgt agacagcttc gcgcctggcc ggctccagcg tcagaaatgc gccgtgcccg
     1081 ccatcgcgac cacgccggcg gcgtttgccg cgggggcaac gggatcgagt atgacggtgg
     1141 atgttgccat cgaggctcct ttcaagtcgt ggggggtgtc gtcaagccgc aacgtgccga
     1201 aaacgggcca cgatcggccc ccatcggcgg atctctccaa gcatgaagcg ccggaattcc
     1261 tcgggcgagc tgccgcacgg cgcgacgccg gcggcgtgga gagggcgaac caccgcctcg
     1321 ctccgcatga cgtcgcgaac ggctgcgctc aactccgcga gcgtggcggg cggcgttgct
     1381 tttggcgcca cgatgcccag ccagttggac acctcgtagc cgggcaggcc cgactccgct
     1441 atggtgggca agtcgggcgc cgcctcgctg cgctccagag ctgaaatgcc gagcggacgc
     1501 agggtgccgg cacggcaccg gggcagcatc gacatgatat tgttgaagct cagggacacg
     1561 agacccgatt cgagctcttc gtaaagccgt tcggtatggt cgtagcgcgc gtggcgcatt
     1621 tccacgccgg ccatttcctg aaacagctcg gccgccaggt gaggcgctcc gccggtggcg
     1681 gaggtcgcgt aggccagttc gcggggacgg gcgcgggcta gctcgatcag ctcgcgcgcc
     1741 gagcccgccg ccacagcagg gtggcacgcc aacagcatgg gggcccgtga caacagggaa
     1801 accggggcga agtcaacaag cgggtcgtag gaaagccgtg ggtcgaggta cggcgcgagc
     1861 gcgtgcgtgc ccagcgttgc cacgaatagg gtgtgaccgt ccgctgcgcc cgccgcgact
     1921 tcgctcgccg caaccgcgcc gttccttccc ggccggcgct cgatccgaac gggcttgccg
     1981 atgcgctcgg cgagcggccc cgcgatgagc cgggcgacct ggtccgaggc gctgccggga
     2041 gaaaagccca gcaccagccg caccgggcgt cgtcgcggcg gttgcctcat tgcgccgtga
     2101 tccgcgcccg ctggaccacc tttgcccact tttcgatctc gcttctcagt ccaccgaagc
     2161 cccggaggac ttcacgacct tcgcccattt tggaatctcg tttctcacat agctcgagaa
     2221 ttccgcgggt gagcttccaa cgatctcgaa accctggttc gaaatccgct cccgtacccc
     2281 cggcgcattg agagcggcga caaatgcggc atgaagcttg ccgacgacgg aagccggggt
     2341 gccccgtggc accatgacgc cgaaccaggt ggtcgcttcg aagccgggga gcccggcctc
     2401 gccgacggtc gggatgtcag gcgccagcag ggtgcggcgg gagctggcga tcgcaagcgc
     2461 tttcagccgg ccgctcgtgg tgtagggcag agccgccgaa acggtgaaga aaccggcttg
     2521 aacctggccg gcgatgagat cggtcgcggc cggtgccgcg cccttgtagg ggacatgccg
     2581 gatctcgatt cccgccatgg atttgaagag ctccatggcc atgtggctgg ggccgccgtt
     2641 gccggacgaa gcgtaattca gccggccggg ctttgccctg gcgagcgcga tgaaatcctt
     2701 gaccgaattc gcgttcaagg cgggattgac cgcgagcacg aacggaatcg aagcgagctg
     2761 ggtgacgggc gtgaagtctt tcaggatgtc gtacggaggc ttccggtaaa gcgtggcgct
     2821 gatggcatgc gccacgttgc cctgcagcag ggtgtggccg tcgggcgcgg ctttcgccac
     2881 gacctccgcg gcgatattgc cgcccgcccc ggcgcggttg tcgatcacga tctgctggcc
     2941 aagcttctcg ccggcgggct gcccgacgat gcgtgcgagg ttgtccgcgc ccccgccggg
     3001 cgggaacggg atgacgaacc gcaaggggcg cgccgggtac ggttgcgcga aggccgcccc
     3061 ggaagccagg gggatcgcgg cgacgatgac tgcgcacagg atcgctgaac gggcaacctt
     3121 cacggtgtcc tccttctcga cggcgggcgc cggtcgcgtc ctatctgacg ggcgccgccg
     3181 cgcgcaccgc gcggcgggtt gcggattcgg tgatcgcttt ttccaccacc gagcgtctca
     3241 gatgcggcac gaacatctcg atgaacgagt acatgtagcc tctcaggtaa tgattccgcc
     3301 tgatgccaac gtggatcgtg ttcggttcga acaggtggct ggcgtcgatg ccgcgcaggc
     3361 tcttgtcgcg ggcacggtcg aacgccatca cgggaacgat ggcgatgccc aggccggact
     3421 ccacgtaagt cttgatcacg tcggcgtcga tggcgttgag cacgatgttg ggaacgatgc
     3481 ctttcgcctc gaacgcgcgc aggatcttcg agcggccgat gaacgcgtag tcgtaggtga
     3541 tcagcggata agcggcgagc ttctccaggg tcagccgccg caccttgagc aatggatgcc
     3601 gggcgggcgt gagcacgatc cgctcaagct gatagcaggg gagcagcacg acgtccgccg
     3661 gcgggtcgag cggctcggtc gcgatggaca gatccgcgct tccggacgag accaggtacg
     3721 aaacctcggc cggcgtgccc tgccgcaggc tcagctgcac tttcggatac cgctgggcga
     3781 agcgcttgat cacctcgggc agcgcgtagc gggcctgagt gtgcgtggtc gcaatgatca
     3841 gcgtgccctg atcctgcccg gaatgctcct tcgccgcctc cttgatactg cgggcgtcgt
     3901 tgagcgtccg ttcggcgagg gaaacgatct ggctgcccgc ctggctcagc cgcaccagcc
     3961 gcttgcggtt gcggacgaac aggtccacct cgagctcccg ctccagcagc tgcagctgcc
     4021 ggctcaggcc gggttgcgag acatgcatgg cttgcgccgc cttggatatg ttcaggttct
     4081 ggcgtacgac ctcgcaaagg tatcgtagct gctgcagcgt catcgggaaa attccataac
     4141 tgtagatttg attagtataa cttttcattg tttgtaagca tatccggttg gccgtatagt
     4201 gccggccaac ggcgcctcgc gcggccgcat gcggaggagc gggcaaggca catgatgttg
     4261 ctggagcacg aaggaaagcg gttgctgggc gcggtcggcg tgccggtgcc ggacggcgtg
     4321 gtcgtcgcgg cgcagccgga atctctgcgt gacgcgggcg cgcggatctc gtcctatccg
     4381 gttgccgtga aggcgcaggt ccgcggcggc ggccgcggca agcggggcgg cgtgcagcag
     4441 gtgatgacgg cgggagacct ggccgccgcg gtaaggaaac tgctcgacac cgagttcggc
     4501 ggggagaagc cggaagcggt gctggtcgag ccctggctcg cgatcgagcg ggaaacctat
     4561 ctttcggtcg cggtggacgt tcgcgccgac ggctacgtgg tgatgtactc gccgaagggc
     4621 gggatcgaca tcgaggacgg cccgccgccg gcccgctatc cggtcggggt gccgtggaat
     4681 ttcagggttc acggcctgcg cagggtgctg gaaagcgtcg agccggacta ccagtggcgc
     4741 gagaaggtca ttaccctcgc gcaacgtctc gtgcgcctgg ccgcgatgcg cgactgcacc
     4801 accatcgaaa tcaacccgct cgtgaggctg gcggacggct cgctcattgc ggcggacgcc
     4861 aaggtggctt gcgacgagtg ggcccacttt cgcaaccggg aaatccatgc ccggcgcgag
     4921 gccgagaaag cgcgcgccaa ccgtctgctg cgcgactgtc tgcagatgca gcatatgtac
     4981 gtgcggctgg acggtgacat tgccctcatt tccggcggcg ccggcatgac catggcggca
     5041 atggacatga tcgcggacta cggcggctgc cccgcgtgct ttctcgattg cagccccggc
     5101 ccgacctcga cgcgcggcta ccggccggcg ttcgcgatgc tcgacgcgga tccgcaagtg
     5161 aaggtgatat tggtgagcgt tttcggcggc ggcacccaga tgcagcgcgt cgccaattcc
     5221 atgaaggaaa tcatggccga acgcaggagc gcgaaaccgg tggtgttccg gctcgacggc
     5281 acgaatgtcg accaggtgtc gggcatcttc gcccagttcg gcgcgcacaa ccatgcgcgc
     5341 ctggaagacg cggtcgcaga agccgtgagg ctggcggcag ggaaaaaatg agcatacttc
     5401 tcgaccgcac caaccgcgtg ctggtccagg gcgtgaccgg acagatgggc acgttttttg
     5461 tgcaggacgc gcgccgctac ggaacgacgg ttgtcgccgg tgtatctcca gggcgcgcag
     5521 ggacgatggt tggcgacgtc ccggtttttg gcagcgttcg tgaggcgcgc gaggcgaccg
     5581 gcgccgatac ggctattgtt ttcgtgccgc cggcagccgt gctcggcggc gcgctggagg
     5641 cgatcgaagc cggctgccgt ctggtggtcg tcactgccga cgagcttccg gttctcgacg
     5701 cgatcgagct gagggccgcg gcccgagcga acggagcggc tttggtgggg ccgaactcgc
     5761 cgggcctcat ttccccgggg aaggcgaaac tcgggttcat gccgtcgttc tgctatatgc
     5821 agggtgatgt cggcgtgatt tcgcgcagcg gctcgctttc gtacgaatgt tgcaagcggc
     5881 tgacgctcgc gggcatgggg cagtccaccg tcgttggcat cggcggcgac gcggtgaagg
     5941 gcacgaccgc cgcggaggcg ctgcagctct tccacgatga tccggagacg tcagccgtcc
     6001 tctatctggg cgagatcggt ggctccgagg aaatattcgt ggccgaatat gcgatccggc
     6061 cagacgcaaa accggttgct gcgctgatcg taggaaaaac ggcgccttcc ggcaggaaaa
     6121 tggggcatgc cgccgcactg atcggttcga aagccgaagg gcacggcgca aagaccgcgc
     6181 tcctgcgcga ggcgggggtg cgggtggcaa gcgggctgga gcaggtcgca acggtaacgc
     6241 gggaggccct cgattcccgg cgcccggcgg ctgcggcggc gtaacgcgac acggacgggc
     6301 tgcgagcgag aaaggagaag cacgatgaac aagcgcgttg atcttggcat cacggaacag
     6361 gccgcggaag ggctgataaa cgaccaatcg ctggctgcgg ctcgagcgct ggtgggggcg
     6421 aagctgcgcc cggagcagta tctgcgcgac gccagcgtgg actcgatcac catcttcgcc
     6481 aacggcatcg gcgatctgaa cccgctctac cgggaccagg agtacgcgcg ctggacgcgc
     6541 tttggcggca tcatcgcgca tccgtgcttt ccgtggacgc accactggcc cgggcgctcc
     6601 tactggggcc tgccgggcgt gcacggattc ggggtggcga tcgactgcga gttctaccgg
     6661 aacgtgcgcc ccggcgaccg gatcaatatc tggaaccggg tcctcggggt ggaggagaag
     6721 ccctcgaagt tttcgggcag gatggcgatg cagtatctgg aatcgaccta caccaatcag
     6781 cgcgacgaga tcctgtgccg cgcgctgggc ctgaccgcgc gccacgagcg caaggcttcc
     6841 cgcgaaaaag gcaagtacaa ggagatcaag actcacgtct atacccccga ggagcgcgcg
     6901 cggatcgacg acatggtgct cagcgaaccg tcgcgcatac gcggcgtcga gacccgttac
     6961 tgggacgacg tcaaggtcgg cgagaaaatc gacgagatcg cgcggggtcc gctctcgatg
     7021 tccgacacca tggccttcgt catcgcttcg ggccggggcg cggcgcacgg cgcgctgctg
     7081 aggcacgccg cgaagcaccc gaaacactac atccgcaaca agcaggcggg cggcggcatc
     7141 gagtacaccg gcatcggcca ccaccgcgag gacttcgcca gggaagtcgg tgtgccgggc
     7201 atgtacgatt acctgccgca gcgcgcgtgc tggtttgcga ccgctgtcac caactggatg
     7261 ggtgacgatg gcgtgctcaa gcgcctgcgc atggaggcgc gcatgttcaa ctgccagggc
     7321 gatacgacat tcatctgcgg tagcgtggtg aagaagtacg tgaaggatcg ctgcgcgctc
     7381 gtggacatcg agatgaaggg cgtcaatcag cgcggggatc tgacctcgcc gggctttgcg
     7441 accgtgatgc tgccggcgcg cgatatccat acccggatcc cgatcgacgg cacggtggtc
     7501 gagctggacc tgccgccgat acgctgagcg gacgaggagc cgatgtccat ggcgaccgac
     7561 gcgatgtacc tcgtctgcga catgatcaac gatctggtgc acgaggacgg cccgaacggc
     7621 aagaagggct atggtcccga gctgaagcgg cgcaatacca ttgccaacac cgcggaggcg
     7681 ctgcgcaagg cgcgggcggc gggggtgctg atcggctacg tgcgcgtggg attctccccc
     7741 gattatcgcg aatgtccccc gacatcgcgc atgttccagg gcgcgaagaa ggcggggatg
     7801 ttcaagctcg gtagctgggg gacggaggtc catccggcgc tggcgcctca gcccggggac
     7861 cacgatatcg tcaagcgccg cgtgagcccg ttctacgcga ccagcctcaa agcgatcctg
     7921 cgcgcgaacg gcatacgccg tctttatctt tccggggttt cgaccagcgg agcggtgctg
     7981 tccggcgcga aggacgggca cgaccgcgac tacgacgtgt acgtgatcga ggattg
//
LOCUS       DBNGWZ010000613         4756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1986681, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000613 DBNGWZ010000000
VERSION     DBNGWZ010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1986681"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1049)
                     /locus_tag="AC16CX_18960"
     CDS             complement(90..1049)
                     /locus_tag="AC16CX_18960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014623.1"
                     /translation="MLRFIFHRFLLMIPTLLGVAVLVFFLLRLMPGDPVATMLLGDAG
                     GANIPREVIEQERARLGLDQPLWVQFFRWFAGAVQGDFGYSMWTGQPVAYEIAIRLEL
                     SLQVAVMATILAILLALPLGTLSAIFKDSWIDQSIRVFSIAGLAVPSFWLGMIIILLL
                     LTYFSWIPPLTFTSFVEDPWENLSQLIWPALAVGYRYSAVSTRMMRSSILEVLQEDYI
                     RTARAKGVVERLVVARHAMRNALLPVVTVIGLEFAFLIGGLVVTEQVFNLNGIGKLFV
                     QAVARSDFTMIQALVLLVSVFFVLVNFVMDLMYAFLDPRIRYR"
     gene            complement(1144..2724)
                     /locus_tag="AC16CX_18965"
     CDS             complement(1144..2724)
                     /locus_tag="AC16CX_18965"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014624.1"
                     /translation="MKRLSMVLLSMAAFAAAGAMAAETPKSGGLLKFAVGAEPPNYDC
                     HAQTSFAFIHPVRPHYNTLLEFDTARYPAIKGDLAESWTVSKDGLTYTFKLRKGVKFH
                     DGSTFSSEDIKATYDRLRDPPPGVRSVRQASYADIASIETPDPLTVVFKLKKPNASML
                     TNFASPWDCVYSAAKLKQDPKFPERNILGTGPFTFAGHVAGSHWTGRKFDGYFRKGKP
                     YLNGYVAIFISGAPMVNALAAGEVLVEFRGHSPADRDRLLKTLGDKATVQETPWVCSL
                     VVTFNTKKKPFDDARVRRALSLAIDRWGGAQALSKVALVRWVGGILRPGYELAAAEKE
                     LVTYPGFSKDINASRAEARKLLQAAGVPNLTFTLTNRNVAMPYTPVGVFLIDQWRQIG
                     VNVKHEQLETRLYLAAQQRDNPTYDAALDFNCDFMDEPNLQLQKYLSHDRSPINYAQQ
                     TDRTLDDLFDRQSGELDKKKRYALLREFEKRALEQAYTIPTIWWHRIIVLHKQVKGWR
                     ITPSHYVGQDLSDVWLDQ"
     gene            complement(2843..4468)
                     /locus_tag="AC16CX_18970"
     CDS             complement(2843..4468)
                     /locus_tag="AC16CX_18970"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HIR3014625.1"
                     /translation="MELLRMVGIPDAERRLGQYPHQFSGGMRQRVMIAIALSCNPKLI
                     IADEPTTALDVTIQAQILKLMKDLSRDLGIAMVIITHNLGIVARYADRVNVMYAARLA
                     EQGSAADVFARPLHPYTAGLLRSVPRLDKPRGFKLETIEGLPPILLDPQPGCRFAARC
                     PAQQPACVAAPPPLVEVEPHRYSACVRAKEMARVGPTGLGLQSANPQPPVHKALVKAQ
                     PLLKVRDLKTYFEVGAGIKLFKSARLEVRAVDGLSFEVFRGETVGLVGESGCGKTTVG
                     RTLLRLEEATGGEIVFEGANVTRAGGQQLKDYRRKIQVIFQDPFSSLNPRMTIGEIIA
                     EPMRVYKLVPDNKAARAKVAELLTQVGLFEYMAERYPHELSGGQRQRVGIARALAMQP
                     SFIVCDEPVSALDVSIQAQIMNLLEELQQKFKLTYLFIAHDLAVVRHISDRVIVMYLG
                     KVMEIADRDTLYAEPLHPYTKALLDAVPIPDPQIEARREHRVLGGEVPSPLNPPRGCV
                     FHTRCPLASEECKMTIPELREVRPRHFAACIKI"
     gene            complement(4557..4706)
                     /locus_tag="AC16CX_18975"
                     /pseudo
     CDS             complement(4557..4706)
                     /locus_tag="AC16CX_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
BASE COUNT          840 a         1551 c         1542 g          823 t
ORIGIN      
        1 gaccgcatgc cgccgccttg gcactttgta agtaatcgct gctgcggtcg tcgtgctcat
       61 cactcatcac ccgtcactca tctcccgcgt tagcgatagc gtatgcgggg gtccaaaaag
      121 gcgtacatca gatccatcac gaagttcacc agcacgaaga acaccgacac cagcagcacc
      181 agcgcctgga tcatggtgaa gtcgctgcgc gccaccgcct gcacgaagag cttgccgatg
      241 ccgttcaggt tgaatacctg ctcggtcacc acgaggccgc cgatcagaaa cgcgaactca
      301 agcccgatca cggtcaccac cggcagcagc gcattgcgca tcgcgtggcg cgccaccacc
      361 aagcgctcga ccacgccctt cgcgcgcgcg gtgcggatgt agtcctcctg cagcacctcc
      421 aggatcgagg agcgcatcat gcgggtggag accgccgagt agcggtagcc caccgcgaga
      481 gcgggccaga tgagctgcga caggttctcc cacgggtcct ccacgaacga ggtgaaagtg
      541 agcggcggga tccacgagaa atacgtaagc agcaggagga tgatgatcat cccgagccag
      601 aacgaaggca ccgcgaggcc cgcgatcgag aacacgcgga tcgactggtc gatccaggaa
      661 tccttgaaaa tcgccgagag cgtgcccagc ggcagggcga gcaggatcgc gaggatcgtc
      721 gccatcacgg cgacttgcag cgacagctcg agccggatcg cgatctcgta ggccaccggc
      781 tggccggtcc acatcgaata gccgaagtcg ccctgcaccg ctcccgcgaa ccacctgaag
      841 aactgcaccc acaacggctg gtcgagcccg agccgcgcgc gctcctgctc gatcacttct
      901 ctggggatgt tggcgccgcc ggcgtcgccc agcagcatcg tcgccaccgg atcgccgggc
      961 atcaggcgca gcaggaagaa caccagcacc gcgacgccga gcagcgtcgg gatcatcagc
     1021 aggaagcggt ggaatatgaa acgtaacact gcggaattcc aaatacgggg ctagggactt
     1081 ggggctagca agacctgatt ctcgccagcc ccgagctccg aggaacgact cccgatcttc
     1141 agattactga tccagccaaa cgtccgacag gtcctgcccc acgtaatggc tcggcgtgat
     1201 gcgccagccc ttcacctgct tgtgcagcac gatgatgcgg tgccaccaga tggtcgggat
     1261 ggtgtacgcc tgttccagcg cccgcttctc gaactcgcgc aggagcgcgt agcgcttctt
     1321 cttgtcgagc tcgcccgact gcctgtcgaa gagatcgtcg agcgtgcggt cggtctgctg
     1381 cgcgtagttg atcggcgagc ggtcgtggga gaggtacttc tgcagctgca ggttgggctc
     1441 gtccatgaag tcgcagttga agtcgagcgc ggcgtcgtag gtcgggttgt cgcgctgctg
     1501 cgccgcgagg tagagccggg tttccagctg ctcgtgcttg acgttgaccc cgatctgccg
     1561 ccattggtcg atcaggaaca ctccgaccgg ggtgtacggc atggcgacgt tgcggttggt
     1621 gagcgtgaag gtgaggttcg gcacgcctgc cgcttgcagg agcttgcgtg cctccgcccg
     1681 cgaggcgttg atgtccttcg agaagccggg ataggtaacc agctccttct cggctgccgc
     1741 tagctcgtag ccggggcgca ggatgccacc gacccatcgc accagcgcaa ccttggacag
     1801 cgcctgcgcg ccaccccacc ggtcgatcgc gagcgagagc gcgcgccgca cccgcgcgtc
     1861 gtcgaacggt ttcttcttgg tgttgaaagt gacgaccagc gagcataccc acggcgtctc
     1921 ctgcaccgtt gccttgtcgc cgagcgtctt cagcaggcgg tcgcgatcgg ccggcgagtg
     1981 gccgcggaat tcgaccagca cctcccccgc ggcgagcgcg ttcaccatcg gcgcgccgct
     2041 gatgaagatc gcgacgtagc cgttgagata gggtttgcct ttcctgaaat agccgtcgaa
     2101 cttgcggccg gtccagtgcg agccggcgac gtgcccggcg aaggtgaagg gcccggtgcc
     2161 caggatgttg cgctcgggga acttcgggtc ctgcttcagc ttcgcggcgc tgtagacaca
     2221 gtcccagggg ctcgcgaagt tggtgagcat ggaggcgttc ggtttcttca gcttgaacac
     2281 cactgtaagc gggtcgggcg tttcgatcga cgcgatatcg gcgtagctcg cctgccgcac
     2341 cgagcgcacg cccggcggcg ggtcgcgcaa ccggtcatag gtcgccttga tgtcctcgga
     2401 agaaaacgtc gagccgtcgt ggaacttgac gcctttcctc agcttgaagg tataggtcaa
     2461 tccgtccttc gacaccgtcc acgattctgc gaggtcgccc ttgatcgccg gatacctggc
     2521 ggtgtcaaac tcgagcagcg tgttgtaatg cggccgcacc gggtggatga aggcgaacga
     2581 cgtttgcgca tggcaatcgt agttgggcgg ctcggccccc acagcgaact tgagcaggcc
     2641 tccgctcttc ggcgtctcgg ccgccatcgc gcccgcggcg gcgaacgccg ccatgctcaa
     2701 cagcaccata gacagtcgct tcatgttccc tcctcctttt cttgataaag cgtgatgggt
     2761 gatgggtgat gggtgatggg tgacggctgc cgcgcatcgc gcggggctct tatcactcat
     2821 cactcatcac ttatcacgca tttcagattt taatgcaggc cgcgaaatgc cgcggcctga
     2881 cttcccgcag ctcgggaatc gtcatcttac actcctcgct cgccaggggg cagcgcgtgt
     2941 ggaacacgca gccgcgcggg ggattgagcg ggctcggcac ttcgcctccc agcacgcgat
     3001 gctcgcgcct cgcctcgatc tgtgggtcgg ggatcggcac cgcatcgagc agcgccttgg
     3061 tgtagggatg cagcggctcg gcgtaaagcg tgtcgcggtc ggcgatctcc atcaccttgc
     3121 cgagatacat gacgatcacg cgatcggaga tgtgccgcac caccgcgaga tcatgcgcga
     3181 taaagaggta ggtcagcttg aacttctgct gcagctcttc cagcaggttc atgatctgcg
     3241 cctgaatcga gacgtcaagc gccgacaccg gttcgtcgca cacgatgaat gaaggctgca
     3301 tcgcgagagc gcgcgcgatc ccgacgcgct ggcgctgccc gcccgagagc tcgtggggat
     3361 agcgttccgc catgtactcg aacagcccca cttgcgtcag cagctccgcc accttagcgc
     3421 gcgccgcctt gttgtcaggg acgagcttgt acacccgcat cggctcggcg atgatctcgc
     3481 cgatggtcat gcggggattc aacgagctga acgggtcctg gaagatgacc tggatcttgc
     3541 ggcgatagtc cttgagttgc tgtcccccgg cacgcgtgac gttggcgccc tcgaacacga
     3601 tctcgccgcc ggtcgcctcc tcaagcctca agagcgtgcg cccgaccgtg gtcttgccgc
     3661 agccggactc ccccaccagc cccaccgtct cgccgcggaa cacctcgaag gagagcccgt
     3721 ccaccgcgcg cacctcaaga cgggcgctct tgaatagctt gatgccggcg ccgacctcga
     3781 agtaggtctt gaggtcgcgc accttcaaca gcggctgcgc cttgacgagc gccttgtgca
     3841 ccggcggctg cggattcgcg ctctggagtc ccagcccggt cggtcccacc cgcgccatct
     3901 ccttcgcgcg gacacaggcc gagtagcggt gcggctcgac ctcgacgagc ggcggcggcg
     3961 cagcgacgca cgccggctgc tgcgccgggc agcgcgcggc gaaacggcat cccggctgcg
     4021 ggtcgagcag gatgggcggc aggccttcga tggtctcgag cttaaagccg cgcggcttgt
     4081 cgagccgcgg caccgagcgc agcagccccg cggtgtaagg atgcagcggc ctggcgaaca
     4141 cgtcggcggc gctgccttgc tccgcgagcc gcgcggcgta catcacattc acccggtcgg
     4201 cgtagcgcgc aacgatgccg aggttgtggg tgatgatcac catcgcgatg ccgagatcgc
     4261 gcgagagatc cttcatgagc ttcaggatct gcgcctggat ggtgacgtcg agcgcggtgg
     4321 tcggctcgtc ggcgatgatg agcttgggat tgcacgagag cgcgatcgcg atcatcacgc
     4381 gctggcgcat gccgcccgag aactggtgcg ggtactggcc aagccggcgc tcggcgtcgg
     4441 gaatgccgac catcctgagc agctccaccg cgcgcgcgcg ccgccgccgc ctcgctcatc
     4501 ccgaggtgga tcagcagcgg ctccatgatc tggaagccga tggtgagcac gggattgagc
     4561 gaagtcatcg gctcctggaa gatcatcgcg atgtcgcgcc cgcggatctc gcgcatctcg
     4621 tcttccggca ggtcgagcag gttcacgccc tggaacagga tgcggccggc gacgatgcgc
     4681 ccggcggggc gcgccagcag ccgcatgatg gcgagcgcgg acaccgactt gccgcagccc
     4741 gattcgccga cgagcg
//
LOCUS       DBNGWZ010000614         4654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1860947, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000614 DBNGWZ010000000
VERSION     DBNGWZ010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1860947"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1575
                     /locus_tag="AC16CX_18980"
     CDS             319..1575
                     /locus_tag="AC16CX_18980"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HIR3014626.1"
                     /translation="MSMIRSTERILTTHAGSLPRSDELRQMVFARAEGRPYDREALAE
                     RLRNEVAEVVRKQSACGIDSVNDGEIGKTNFTNYVRGRLSGYESRPYRPGKDPEPLSI
                     AGRDRIRFPEYFAAGGRGFGAFAGAGPSQTQVYCVAPLRYVGHEALREELEVFRAALA
                     GVKIGEAFLPANTPGTIEHWLRNEHYPDEEAFLYAIAEVMRVEYKAIIDAGFLLQIDD
                     PDLPDGWQMYPAMNVADYRKYAALRVDALNHALRGIPRESIRLHVCWGSFHGPHQHDI
                     PLKDIIDIIFQVRAGAYSIEASNPRHEHEWRVFEGVRLPEGALLIPGVVGHCTDFIEH
                     PELVAERLVRYAGLVGRENVMGGTDCGLGPRVGHPRIAWAKLEALAEGARLATKRLWV
                     RSRRAKRTRPSGRRASHSTRPRRRRT"
     gene            complement(1493..2317)
                     /locus_tag="AC16CX_18985"
     CDS             complement(1493..2317)
                     /locus_tag="AC16CX_18985"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014627.1"
                     /translation="MSLFIPAGFAQDKPAAQEYQPEVGQAGKDVVWVPTPQALVDKML
                     DLAKVTSRDYVIDLGSGDGRTVITAAKRGARTLGIEYNPDMVALSQRNAAKEGVADRV
                     KFVKADLFESDFSQATVITMFLLPDINLKLRPKILDLKPGTRIVSNSFTMGEWQHDRS
                     VKATEQEGCQSYCTAYLWIVPAKVDGTWTLPDGELKLKQSFQMITGTLTAGGRETAVK
                     GRLRGDQISFKAGEAQYSGRVTGDAIKGTVKSGGGAGEWSATRAGRTAASALPAGS"
     gene            complement(2529..4310)
                     /locus_tag="AC16CX_18990"
     CDS             complement(2529..4310)
                     /locus_tag="AC16CX_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR3014628.1"
                     /translation="MTPSFVAPPRGDLTHLLRLGRFLVPYRWRIAAALTALVIAAGCV
                     LVLGQGLRHVVDAGFGSGDPRLLNAALAAVVAVSVVLAAATWIRFYLMMSVGERIIAD
                     LRMSVFAHVITLTPAFFDSSRTGEIASRLTNDSEQLRQVIGFGFSMFLRNGLMMLGAL
                     VLLFATSLKLAALIVLGVPATLVPILLMGRRVRRLARVNQDRVADVSAHVDESLHEIR
                     TVQAYGHEAHTRDQFDVATDAACVAGVHRVRVKAWLISLVMLIGFCAVGVILWIGGHD
                     VFAGRMTAGELSAFVFYAVVVASGAATVSEVWGEIQRGAGATERLTELLDTPPALAAA
                     TPVIKLPPRVAGAVRFDEVVFSYPARPGTIALGPVSFAVSPGERVALVGPSGAGKSTI
                     FALILRFYDPASGRILLDGADLRHCEPLDLRRAVALVPQDPVIFATSVTENVRFGHPG
                     ASLETVREACAAAHALEFIEHLPQGFDTDLGERGVKLSGGQRQRISIARAILADRPIL
                     LLDEATSSLDAESERQVAQALERLARGRTTLVIAHRLATVRNADRIIVLDRGRVHAVG
                     THNELLRASELYAHLARLQFIAEGETA"
BASE COUNT          834 a         1524 c         1557 g          739 t
ORIGIN      
        1 ctcatgcggc gcgaggatgc ccgctgggcc gcggtgatcc ggaagaacaa catcaaggct
       61 gagtaaaacc gccgatgaac gcggatgaac acagatagac caatccctga agaagtcatc
      121 ggcaaggatt ttcatcgcgt ttatccggag ttatccgggt cagtccctcc tagccattga
      181 aatgtgtctg gaacttgcct tagaaatcaa aaacgggatt actgcagatc aacgccgata
      241 ttaatcagag caatatagtg ggtttatttt gaatttgtcg gcattggtcg gcgttcatcg
      301 gcggttcccg agttttggat gtccatgata cgcagcacgg aacggatcct gaccacgcac
      361 gccgggagcc tgccgcgctc cgacgagctg cgccagatgg tcttcgcccg ggcggaaggg
      421 cgaccctacg acagggaagc cctggccgaa cggctgcgga acgaagtcgc cgaagtcgtg
      481 cgcaagcaat ccgcgtgcgg catcgacagt gtcaacgacg gcgagatcgg caagaccaac
      541 ttcacgaatt acgtccgcgg gcgtctttcc ggttacgagt cgcggccgta ccggccgggc
      601 aaggacccgg agccgctttc catcgccggc cgggaccgca tccgctttcc cgagtatttc
      661 gccgcgggcg ggcgcggctt cggcgccttc gccggggccg ggcccagcca gacgcaggtc
      721 tactgcgttg cgcccctacg gtatgtcggc cacgaggcgc ttcgagagga gctggaagtc
      781 ttcagggcgg cgctcgcggg cgtgaagatc ggggaagcgt tcctgcccgc caacaccccg
      841 ggcacgatcg agcactggct gcgcaacgag cattaccccg atgaagaggc cttcctctac
      901 gcgatcgcgg aggtgatgcg cgtcgaatac aaggcgatca tcgacgcggg tttcctgctc
      961 cagatcgacg atcccgacct gccggacggc tggcagatgt atcccgcgat gaacgtcgcg
     1021 gattaccgga agtacgccgc gctccgcgtc gacgccctga atcacgcgct gcgcggcatt
     1081 ccgcgcgaga gcatccggct gcacgtgtgc tggggcagct tccacgggcc ccaccagcac
     1141 gacatcccgt tgaaggacat catcgacatc atcttccagg tgcgcgcggg ggcctattcg
     1201 atcgaggcct cgaatccccg ccacgagcac gagtggcgcg tgttcgaggg cgtcaggctg
     1261 ccggagggcg cattgctcat tcccggcgtc gtcggccatt gcaccgactt catcgagcac
     1321 ccggaactcg tggccgagcg cctcgtgcgc tacgccggac tcgtcgggcg ggaaaacgtg
     1381 atgggcggga cggactgcgg cctcggcccg cgggtcgggc accctcggat cgcatgggcc
     1441 aagctcgagg cgctggccga aggcgcgcgc ctcgctacga agcggttgtg ggtcaggagc
     1501 cggcgggcaa agcggacgcg gccgtccggc cggcgcgcgt cgcactccac tcgcccgcgc
     1561 cgccgccgga cttgacggtg cccttgatcg cgtcgccggt gacgcgcccg ctatactggg
     1621 cctcgccggc cttgaagctg atctggtccc cgcgcagccg tcccttcacc gcggtttccc
     1681 tcccgccggc ggtgagcgtg ccggtgatca tctggaagct ctgcttgagc ttgagctccc
     1741 cgtccggcag cgtccaggtt ccatccacct tcgccggcac gatccagaga taggccgtgc
     1801 agtaggactg gcagccctcc tgctcggtcg ccttcacgct gcggtcgtgc tgccactcgc
     1861 ccatggtgaa ggaattcgac acgatgcgcg tgccgggctt gagatcgagg atcttcgggc
     1921 ggagcttcag gttgatgtcg ggcagcagga acatggtgat cacggtcgcc tgcgagaaat
     1981 cgctctcgaa gagatcggcc ttcacgaact tcaccctgtc ggcgacgcct tccttcgccg
     2041 cgttgcgctg cgacagcgcg accatgtcgg ggttgtattc gatgccgagg gtgcgggctc
     2101 cgcgtttggc ggcggtgatg acggtgcgcc cgtcacccga gccgaggtcg atcacgtagt
     2161 ccctcgacgt gaccttcgca aggtcgagca tcttgtccac gagcgcctgc ggagtcggca
     2221 cccataccac gtccttgccg gcctggccga cttccggctg gtattcctgg gcggccggct
     2281 tgtcctgcgc gaagccggcg ggaatgaata aagacaaaca cagcgccaaa aaagaaaggc
     2341 gcccaaaacg cgttgcgatc atgttgattc tccgttcagg ttaaaggcgc gggaaccatg
     2401 aaacagcccc ggccgacgat gggtttcgcg atgatatcgg attaccggcg taaccggtaa
     2461 tccgcagcaa tattcttgtt caggcgaaac tgcgtgtgcg ttgtctccga tatcctggta
     2521 gccggggttc acgcagtttc gccttcggcg atgaattgca gccgcgcaag gtgcgcgtag
     2581 agctcgctcg cgcgcagcag ctcgttgtgc gtcccgacgg catggacgcg cccgcggtcg
     2641 aggacgatga tgcggtcggc gttacgcacg gtcgcgaggc ggtgcgcgat gacgagcgtg
     2701 gtgcggccgc gcgcaagccg ctccagcgcc tgcgcgacct ggcgctcgct ctcggcgtcg
     2761 agcgagctcg tcgcctcgtc gagcagcagg atcggccggt cggcgaggat cgcccgcgcg
     2821 atcgagatgc gctggcgctg gccgccggaa agtttcacgc cgcgctcgcc gagatcggta
     2881 tcgaagccct gtggcaggtg ctcgatgaac tccagggcgt gcgccgccgc acacgcctcc
     2941 cgcaccgtct cgagcgaagc gccgggatgg ccgaagcgca cgttctcggt gacgctcgtc
     3001 gcgaagatca ccgggtcctg tggcaccagc gcgacggcgc gcctcagatc gagaggttcg
     3061 cagtgccgaa ggtcggcgcc gtcgaggagg atgcggcccg acgccgggtc gtagaagcgc
     3121 aggatcagcg cgaagatcgt ggatttgccc gcccccgacg ggccgaccag cgccacccgc
     3181 tcgccggggc tcaccgcgaa cgataccggc ccgagcgcga tcgtcccggg gcgggcggga
     3241 tacgagaaaa cgacctcgtc gaagcggacc gcgcccgcca cgcgcggcgg gagcttgatc
     3301 accggtgtcg cggccgcgag cgcgggcggc gtgtccagca gctccgtcag ccgctcggtc
     3361 gcgcccgcgc cgcgctggat ctcgccccac acctcggaga ccgtcgccgc gccggaggcg
     3421 accaccacgg cgtagaacac gaaggccgaa agctcccccg ccgtcatgcg cccggcgaag
     3481 acgtcgtgcc cgccgatcca caggatcacg cccaccgcgc aaaatccgat cagcatgacg
     3541 agcgagatca gccacgcctt gacgcgcacg cgatgcacgc cggcgacgca ggccgcatcg
     3601 gtcgccacgt cgaattggtc cctggtgtgc gcctcgtgcc cgtaggcctg caccgtgcgg
     3661 atctcgtgca gcgattcgtc gacgtgcgcg gacacgtccg ccacgcggtc ctggttgacg
     3721 cgggcgagcc gccgcacgcg ccggcccatc agcaggatcg gcaccagcgt cgccggcacg
     3781 cccagcacga tcagcgccgc gagcttgagg ctggtcgcga agagcagcac cagcgcgccc
     3841 agcatcatca ggccgttcct cagaaacatc gagaagccga agccgatcac ctgccgcagc
     3901 tgctcgctgt cgttggtgag gcgggaggcg atctcgccgg tgcgcgagga atcgaagaag
     3961 gcgggcgtga gcgtgatcac gtgggcgaac acgctcatgc gcaggtccgc gatgatgcgc
     4021 tcgcccacgc tcatcatgag atagaagcgt atccaggtcg ccgccgcgag cacgaccgag
     4081 accgccacca cggcggcgag cgcggcgttg agcaggcgcg ggtcgccgct gccgaagccg
     4141 gcgtccacca cgtggcgcag cccttgcccg agcacgagca cgcagcccgc cgcgatgacg
     4201 agcgcggtga gcgccgcggc gatccgccag cggtagggaa cgaggaaccg cccgagacgc
     4261 agcagatggg tgagatcgcc cctgggcgga gcgacaaagg aaggggtcat tgccacgcgg
     4321 ctatcctagc atccgtggcc gtaatggcca cggagaaaag cgtgataggt gatgggtgat
     4381 aagtgataag gcttcaaacg gtgtgatagg taacgagtga taagtgatag gttgcccgcg
     4441 cagcggggga taatagtcct tatcacctat cacttatcac ttatcactcg tcacgccttc
     4501 tcgacctgcg gacgggcgcg cagccgcaga accagcagcg ccacgatcgc aatgagcagg
     4561 agcacggcga tgacgagaag ctgccatatc gggccgcaat ccgacactcc cagcctgccg
     4621 ctggtgcagg attgcaggta ggccaggacg atgc
//
LOCUS       DBNGWZ010000615         7604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3123164, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000615 DBNGWZ010000000
VERSION     DBNGWZ010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3123164"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..754)
                     /locus_tag="AC16CX_18995"
     CDS             complement(71..754)
                     /locus_tag="AC16CX_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3014629.1"
                     /translation="MINVFIGYDPREAVAYSVLAYSIQARASEPVSIAPLMLSQLKTV
                     LTRQRHPLQSTDFSFSRFLTPYLSGYAGWSVFMDCDMLMLDDIANLWKLRDDRYAVMV
                     VKHNHVPKETTKFLGEPQSRYEKKNWSSVMLFNNARCRALTPEYVNQASGLELHQFKW
                     LASDDLIGALPERWNHLVGYSPPRRDAALVHYTLGGPYFSEYRDCEYSKEWLAERDAM
                     QRVANRAMA"
     gene            938..1915
                     /gene="galE"
                     /locus_tag="AC16CX_19000"
     CDS             938..1915
                     /gene="galE"
                     /locus_tag="AC16CX_19000"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR3014630.1"
                     /translation="MTILVTGGAGYIGSHMVKLLRERGRRIIVIDSLVTGHRDAVKDV
                     EFVHADVGDFGKVAALLAERSVRAVIHFAAHGLVSESTANPMKYYANNVGATLGLLGA
                     MREARVEQIVFSSTAAVYGEPRGAPIEESHTKEPINPYGASKLMIERVLDDLHAAHRL
                     RSASLRYFNAAGADPSGQLGERHDPETHLIPLVLQAASSRRPSVAVFGTDYPTRDGTC
                     VRDYVHVNDLCDAHLLALEWLDGGGGREAFNLGSGSGATVMEVIEAAQRETGAPLEVR
                     LEGRRPGDPAVLVASCERARRVLGWSPKRSDITTIVRDAWRWERRSAAA"
     gene            complement(1922..3136)
                     /locus_tag="AC16CX_19005"
     CDS             complement(1922..3136)
                     /locus_tag="AC16CX_19005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3014631.1"
                     /translation="MRCGPAARARGAESVRLLLVTHYYSTHRGGVEVVAHRLADVLLR
                     NEDVAIDWMASDCDPAPEALPERLRCTPARSWNGIERACGLPFPLWSPGAVLRLRRAV
                     RECDAIHLHDALYMGNVLAFLFAAIERKPVIVTQHIGAIPYDSAVLRGLLAGLNRTVA
                     RAILSRAQKVLFVSPAVQQYFAAFCAFRSPPVYAPNGVDGGLYSFVDAEGISALRRAA
                     GRNPERALCLFVGRFVERKGVGLVLSLASALRHVDWILAGDGPMRPEDARLPNVTVIR
                     GRSGRDIAALYQLADLLVLPSKGEGFPLVVQEAMACGTPALVSRETAAGAPAVEQLLF
                     TEDVFAADAAGRWRARLQELLARLDTLRGMRSRLAQAAREQWSWEAAGKAHASAFREA
                     LSMRPVSDAGAD"
     gene            complement(3099..3944)
                     /locus_tag="AC16CX_19010"
     CDS             complement(3099..3944)
                     /locus_tag="AC16CX_19010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HIR3014632.1"
                     /translation="MNSQAGAGSAPRGARRVSAIIPTHNRRGPLAAAIASVLRQTWPL
                     HELIVIDDGSTDGTWEDLRALAAGATAARIVVQRQPNAGPSGARNAGVRMATGEFIAF
                     LDSDDTWHEEKTARQLAVFDSQPDLALVGCVAEGMEVFPGRRIVPISVEQLLFRNYFL
                     TPGVMVRRELLAVVGGFAEDMRHCEDYDLWLRVASAHRCALLNEVLIACGGGKRPFGD
                     SGLSAGLWAIQSGELQAFRRWRDRGGPAAKYWYAIAVSWLRFARRAAVVALDALRPGR
                     ARTRG"
     gene            complement(3941..4777)
                     /locus_tag="AC16CX_19015"
     CDS             complement(3941..4777)
                     /locus_tag="AC16CX_19015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014633.1"
                     /translation="MNVWGEELPEDLLSDADRAFLSRFVSTPRPALEEIWRQLDLVWT
                     EMGMDQEITPASLARYYSHPVWLVNGLFTAADPQSAAHRRAIADWLAGRNVQRVADYG
                     GGFGQLAREIVRAADRIRVDVVEPFPSRVGRFRVAALPRVALVDELAGPYDAVIAQDV
                     LEHVHDPIGLVAQLTRTVVPGGFALFANAFYPFIHCHLPHTFHLRHTFGWVVAALGLR
                     FEARVEGAAHVLVFRRDSGEPDIARARLRERVSRVIGPALNGLRGVAAGLVRRYHAWA
                     EA"
     gene            complement(4774..6021)
                     /locus_tag="AC16CX_19020"
     CDS             complement(4774..6021)
                     /locus_tag="AC16CX_19020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3014634.1"
                     /translation="MGRSDTHGGAARASYRLHRALNAGGHASRMCVAVKESADRTVAE
                     PDGALASAWSLIRPGLANALMRLQRTPDNAWRSPAMFPSGLGRAINRCDADVVNLHWV
                     GEETMSVEEIGRIAKPVVWTMHDMWPFCGAEHYAPEGAHARWRSGYAPGTRDAAARGL
                     DIDAWVWRRKHNAWRPCQLVAPSRWLADCARASALTRDWPVAVVPNVLDTEVFRPCSD
                     ASARDKLGLPPAAPLLLFGAASGTRDPRKGWDLLVAALALLATDHPDLVCIVAGENEP
                     ASAPKLALPVRWLGHVRDAAELAHLYGTVDVTVVPSRQENLPQMATEAQGCGCPVVAF
                     ASTGLPDAVEHGVTGYLAEPFVPADLARGIAWVLADRERHLRLRAQARERAARLWSPG
                     VVTRRYLELYAAAIEAHRVKTVG"
     gene            complement(6033..6977)
                     /locus_tag="AC16CX_19025"
     CDS             complement(6033..6977)
                     /locus_tag="AC16CX_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014635.1"
                     /translation="MTAGRPAGAGPLRTPVLLLVFNRPETTARVIAAIRAARPARFYV
                     AADGPRNAGEAERCEAARRVATQVDWPCDLHTLFRERNLGCRRAVGDAISWFFSREES
                     GIVLEDDCVPSASFFGYCEELLDRYRDDERVMSIGGSNFQKGRRRGPASYYATKHFHC
                     WGWASWRRAWAEYDGELSSVGATLEKGLAQLQDGSAAFKRYWMRIYRDCRAGRYSSWA
                     YPMSLTCFARVGAGRETLHLAPQVNLVANIGFGGGGTHISVDNQNPAATAGELALPLR
                     HPERLVRDVDADCFTDKTHFRIGWAPLLKEKLMRMLPL"
     gene            complement(6989..>7604)
                     /locus_tag="AC16CX_19030"
     CDS             complement(6989..>7604)
                     /locus_tag="AC16CX_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014636.1"
                     /translation="TRPIAALDAVLILNDDLTFGPEFLSAGLGCLQIDRRRWVQAVAY
                     RADRPHPDRGVEADLVRLRFRVAQDASRVNCLSTRGLLIDLAQFVRSGGFRPRSLPHY
                     LSDYEFTLRGARNGALLTSCESFTVRMSPELTGLRTPPDRGFVRFMRSSFSNKATFNP
                     LRLSCFVVLCCPAWVVPIHLIGIWAGFLRKAIMSLMPVRKLSPP"
BASE COUNT         1351 a         2648 c         2466 g         1139 t
ORIGIN      
        1 agaaggtggt gcgctcggtg cagcccaacc gcccggtgct caccggcgtg cagggcaatc
       61 cggggctttc ctaggccatg gcccggttcg ccacgcgctg catcgcgtcg cgctccgcca
      121 accactcttt cgagtactcg caatcccggt attcgctgaa gtacgggccg cccagcgtgt
      181 aatgcacgag ggcggcgtcc ctgcgcggcg ggctgtagcc cacgaggtgg ttccagcgct
      241 cgggcagcgc gccgatcaga tcatcgctcg ccagccactt gaactggtgc agctcaaggc
      301 ccgaggcctg gttcacgtac tcgggcgtga gcgcccggca ccgggcgttg ttgaacagca
      361 tgacgctcga ccagttcttc ttctcgtacc ggctctgcgg ctcgcccagg aacttcgtcg
      421 tctccttggg cacgtggttg tgcttgacca ccatcaccgc gtagcggtcg tcgcgcaatt
      481 tccagagatt ggcgatgtcg tcgagcatca gcatgtcgca gtccatgaac acggaccacc
      541 ccgcgtagcc cgagaggtaa ggcgtgagaa agcgcgaaaa cgagaaatcc gtgctctgca
      601 gcgggtgtcg ctggcgggtg aggactgttt tcagctggga cagcatgagg ggagcgatgg
      661 agaccggctc ggaggcgcgg gcctggatgc tgtacgccag tacgctgtac gcgaccgcct
      721 cgcgcggatc gtagccgatg aaaacgttga tcatgtgctg ccgggcctct gcacgatgaa
      781 ccggacatgt gcggcgcagc gaagcatagc aatccccgcc atccaccgca aacctcccgc
      841 gtcaccacac cgcggccgcc gcgatcaata tctggaaacg cgcgccataa tcagtgaaaa
      901 tgcgggtcat gcgcacgcat ttcctgccga gcttcgattg actattctcg tcacaggtgg
      961 tgcgggctac atcggttcgc acatggtgaa gctgctccgc gagcgcggcc ggcgcatcat
     1021 cgtgatcgac agccttgtca cgggccatcg cgacgccgtc aaggacgtcg aattcgttca
     1081 cgccgatgtt ggcgatttcg gcaaggtcgc ggcgctgctc gccgagcgct cggtccgggc
     1141 ggtgatccac ttcgcggcgc atggcctggt gtccgaatcg accgcgaatc cgatgaagta
     1201 ctacgcgaac aacgttggcg ccacgctcgg attgctgggc gccatgcgcg aagcgcgggt
     1261 cgagcagatc gtgttttcct ccaccgccgc ggtgtatggg gaaccgcgcg gggcgccgat
     1321 cgaggaatcg cataccaagg agccgatcaa tccatacggc gcgagcaagc tcatgatcga
     1381 gcgggtgctc gacgacctgc atgccgcgca taggctgcgc tccgcgagct tgcgctactt
     1441 caacgccgca ggcgccgatc cgagcgggca gctcggggag cggcacgatc cggaaacgca
     1501 cctcatcccc ctggtgctgc aggccgcttc cagccggcgg ccatcggtcg ccgtcttcgg
     1561 caccgattac ccgacacgcg acggcacttg cgtgcgcgat tacgtgcacg tgaacgacct
     1621 gtgcgacgcg cacctcctgg cgctcgaatg gcttgacggc ggaggcggcc gcgaggcctt
     1681 caatctcggc agcgggagcg gtgcaacggt catggaagtg atcgaggctg cgcagcgcga
     1741 gaccggcgcg ccgctcgagg tgcggctcga gggccgccgt cccggagacc cggccgtgct
     1801 cgtcgcaagt tgcgagcgcg cgcgccgcgt gctgggttgg agcccgaagc ggtccgacat
     1861 aacgaccatc gtccgggacg cgtggcgctg ggagcgccgt tcggctgcgg cgtaaggccg
     1921 ctcagtccgc gcccgcgtcg gaaacaggcc gcatcgacag cgcctcccga aacgcggaag
     1981 catgagcctt tccggccgct tcccaggacc actgttcgcg cgccgcctgc gccagccggg
     2041 aacgcatgcc gcgcaaggta tccaaccggg caagaagctc ttgcagccgc gcgcgccagc
     2101 gccccgccgc atctgccgcg aaaacatcct cggtaaaaag cagctgctcc acggccggcg
     2161 ctcccgccgc cgtttcacgg ctcaccagcg ccggcgtgcc gcaggccatc gcctcctgga
     2221 cgacgagcgg aaacccctcc cccttgctcg gcaacacgag caggtccgcc agttgataaa
     2281 gcgccgcgat gtcgcggccg gaccgccccc gtatcaccgt gacattcggc agccgcgcgt
     2341 cttcaggccg catcggcccg tcgccggcga ggatccagtc cacgtgccgc aacgcgctcg
     2401 ccagcgacag caccaggccc acgcccttcc gctccacgaa tctgccgacg aacaggcaga
     2461 gcgcgcgttc cgggttccgg cccgcggccc tgcgcaacgc ggatatgcct tcagcatcga
     2521 cgaaactgta gagcccgccg tccacgccgt tcggggcgta aaccggcggt gagcggaacg
     2581 cgcagaacgc ggcgaaatat tgctgcaccg ccggactgac gaacagcacc ttctgcgctc
     2641 gcgaaaggat cgcgcgcgca accgtgcggt tcaagcctgc gagcaatcca cgcagcacgg
     2701 cgctgtcgta tggaatcgcg ccgatatgct gggtcacgat caccggtttg cgctcgatcg
     2761 cggcgaacag gaacgcgagc acgtttccca tgtacagcgc gtcgtgcagg tgaatcgcgt
     2821 cgcattccct gaccgcgcgc cgcagccgga gaaccgcgcc gggggaccat agcgggaagg
     2881 gcaggccgca tgcccgttcg atcccgttcc aggaccgcgc cggggtgcag cgcagccgct
     2941 cgggcaacgc ttcgggcgcc gggtcgcagt cgctcgccat ccagtcgatg gcaacgtctt
     3001 cgtttctcag caggacatcg gcgaggcggt gcgccaccac ttcgacgccc ccgcgatgcg
     3061 tcgagtaata atgtgtgacg agcaggaggc gcaccgattc agccccgcgt gcgcgcgcgg
     3121 ccgggccgca gcgcatcgag cgccacgaca gccgcccgcc gtgcaaaacg cagccacgac
     3181 accgcgatcg cgtaccagta tttcgccgcc gggccgccgc gatcgcgcca gcggcggaac
     3241 gcctggagtt cacccgactg gatcgcccac agccccgccg acaagccgct gtcgccgaac
     3301 ggacgcttgc cgccgccgca tgcgatcagc acttcgttga gcagcgcgca gcgatgtgcc
     3361 gaggcgacgc gcagccacag gtcgtagtcc tcgcagtggc gcatgtcttc agcgaacccc
     3421 ccgacaacgg cgagcaactc gcgccgcacc atcacgcccg gcgtcaggaa atagttgcgg
     3481 aacaggagct gctcgacgct gattggaacg attcgtcttc ccgggaaaac ctccattcct
     3541 tcggcgacac agccgacgag ggcaaggtcg ggctgggagt cgaacactgc gagctgccgg
     3601 gcggtttttt cctcgtgcca ggtatcgtcg ctgtccagga aggcgatgaa ctcgccggtg
     3661 gccatgcgca cgccggcgtt gcgcgcgccc gacgggccgg cgttgggctg ccgctgcacg
     3721 acaatgcgag ccgccgtcgc gcctgccgcc agcgcccgga gatcctccca cgttccatcg
     3781 gtcgatccgt catcgatgac gatcagctcg tgcagcggcc atgtctggcg caggaccgag
     3841 gcgatcgccg cggccagcgg tccgcgccgg ttgtgcgtcg gaataatggc cgacacacga
     3901 cgcgcgccgc gcggcgcgga tccggcgccg gcttggctgt tcatgcctcc gcccatgcgt
     3961 ggtaacgacg gacaagcccg gcggcgacgc ctcgcaagcc gttgagcgca ggaccgatca
     4021 cgcgcgaaac gcgctcgcgt agccgcgccc gcgcgatgtc cggctcaccg ctgtcgcggc
     4081 ggaaaaccag cacgtgcgcc gcaccctcaa cccgggcctc gaatcgcagc cccagcgccg
     4141 cgaccaccca gccgaacgtg tggcgcaaat gaaatgtgtg cggcaaatgg caatgaataa
     4201 aggggtagaa cgcattggcg aacagcgcaa atccgcccgg aaccacggtc cgcgtcaact
     4261 gcgccacaag gcctatcgga tcgtgcacgt gctcgagcac gtcctgtgcg attacggcgt
     4321 cgtagggccc ggccagctcg tcgacaagcg cgactcgtgg caacgccgcg acgcggaacc
     4381 gcccgacccg ggatggaaac ggctccacga cgtcaacgcg gatcctgtct gcggcacgca
     4441 cgatttccct cgctagttga ccgaaaccgc cgccatagtc ggccactcgc tgcacgttgc
     4501 gcccggccag ccagtccgcg attgcgcgcc gatgggcggc cgattgcgga tcggcggcgg
     4561 tgaacaggcc gttcaccagc cacacggggt gcgagtaata gcgcgcgagg gacgccgggg
     4621 taatctcctg atccatcccc atttcggtcc agaccaagtc gagctggcgc caaatctcct
     4681 ccagggccgg acgtggcgtc gagacgaacc gcgacagaaa cgcgcgatca gcgtcgctaa
     4741 gaaggtcctc cggcagttct tcgccccaga cgttcatcct acggtcttca cacgatgtgc
     4801 ttcgatcgct gcggcataca gctcgaggta gcgccgcgtg acgacgcctg gcgaccacag
     4861 ccgcgccgct cgctcccgcg cctgggcgcg cagccgcagg tgacgctcgc ggtcggcaag
     4921 cacccatgcg atcccgcgcg cgagatccgc gggcacgaaa ggctccgcca ggtagccggt
     4981 caccccgtgc tcgactgcgt ccggcagccc ggtgctcgca aaagcgacga ccggacagcc
     5041 gcagccctgc gcttcggtcg ccatctgcgg caggttctcc tggcgcgacg gcaccacggt
     5101 gacgtcgacg gttccgtaca ggtgcgccag ctcggctgca tcgcgcacat gcccgagcca
     5161 tcgaaccggc agcgccagct tcggcgcaga cgccggctcg ttttcgccgg ccacgatgca
     5221 tacgagatcg ggatgatcgg ttgcgaggag cgcaagcgcc gccacgagca gatcccaccc
     5281 ttttcgcgga tcgcgcgttc cgctggcggc gccgaagagc aggagcggcg cggccggcgg
     5341 caaccccagc ttgtcgcgcg ccgaagcgtc ggagcacggt cggaacacct cggtatcgag
     5401 aacattcggc acgaccgcga ccggccaatc ccgcgtcagg gcgctggcac gggcgcaatc
     5461 ggccagccag cggctcgggg cgacgagctg gcacggtcgc caggcattgt gcttgcgccg
     5521 ccacacccag gcatcgatgt cgagtccgcg cgcggcggca tcccgtgtgc cgggcgcata
     5581 gccgctgcgc caccgggcat gagcgccctc cggcgcatag tgctctgcgc cgcagaaagg
     5641 ccacatgtcg tgcatcgtcc acacgaccgg cttcgcgatg cggccgattt cctcgaccga
     5701 catcgtttcc tccccgaccc agtgcaggtt gacgacgtcg gcgtcgcagc ggttgatcgc
     5761 ccggccaaga cctgacggaa acatcgccgg cgaccgccac gcgttgtccg gcgttcgctg
     5821 caggcgcatc agcgcattcg cgagcccggg acgaatcagg ctccatgcgg acgcgagcgc
     5881 gccatcgggc tccgccactg tgcggtcggc ggattccttg acagcgacgc acatgcgact
     5941 cgcatgcccg cccgcattga gcgcgcggtg cagccggtag ctagcgcgcg ccgcgccgcc
     6001 gtgagtgtcc gaacgaccca caatgaggac tttcaaaggg ggagcatccg catcagcttt
     6061 tccttgagca aaggcgccca gccgatgcga aaatgggttt tgtcggtgaa gcagtcggcg
     6121 tcgacatcgc ggactaggcg ctccgggtgc cgcagaggca gggcgagttc acccgcagtc
     6181 gcggccggat tctggttgtc aaccgatata tgcgtgccgc ctcctccaaa gccgatattc
     6241 gcgacgaggt tcacctgcgg cgccagatgc agcgtttcgc gccccgcgcc gacccgcgcg
     6301 aagcacgtaa gcgacatggg atatgcccag ctggagtagc gcccggcgcg gcaatcccga
     6361 tagatccgca tccagtatcg cttgaacgcc gcagatccgt cctgcagctg cgccaatccc
     6421 ttctcgagcg tcgcgcccac ggacgaaagc tcgccgtcgt attcggccca ggcgcggcgc
     6481 cacgacgccc acccccagca gtgaaagtgc ttcgtggcgt aatacgatgc cggcccgcgg
     6541 cgccggccct tctggaaatt actcccgccg atgctcatga cgcgctcgtc atcccgataa
     6601 cggtcgagca gctcctcgca gtagccaaag aagctcgcgc tcggcacgca gtcgtcttcg
     6661 agcacaatgc cggattcttc ccgcgaaaag aaccacgaaa tggcgtcgcc gaccgcgcgg
     6721 cggcagccca gattgcgctc gcgaaacagg gtatggagat cgcacggcca gtcgacctgc
     6781 gtcgcgaccc gccgcgcggc ttcgcaccgt tccgcttccc ctgcattgcg cggcccgtcg
     6841 gcagcaacgt agaatcgcgc cgggcgggct gcccggatcg ccgcaatgac tcgcgcggtc
     6901 gtttcggggc ggttaaacac gagcagcagc acgggggtcc gtaacggccc tgcgccggcc
     6961 gggcggcccg cggtcatggc acgatgcatc atggcggcga gagcttgcgc acgggcataa
     7021 gcgacattat cgctttgcgc agaaatccgg cccaaatgcc gatcaaatgg atcggcacga
     7081 cccaggccgg gcagcacaga acgacaaaac agctcagtcg aagcggattg aacgtggcct
     7141 tgttggagaa cgaggagcgc atgaagcgca cgaatcctct gtccgggggc gtccttaaac
     7201 cggtcaattc cggactcatg cgcaccgtga acgattcgca gctggtcagc agggccccgt
     7261 tccgcgcccc acgcagcgtg aattcgtagt cgctcaggta gtgaggcaac gaccgcgggc
     7321 ggaacccgcc cgagcgcacg aactgcgcga ggtcgatcag caggccgcgc gtcgatagac
     7381 aattgacccg tgaggcgtcc tgcgcgacgc gaaatcgcaa gcgcacgaga tcggcctcga
     7441 cgcctcggtc cggatgcggc cggtcggcgc ggtatgcgac cgcctgcacc cagcgccgcc
     7501 ggtcgatttg cagacagccc aacccggcgc tgaggaattc cggaccgaac gtcaaatcgt
     7561 cgttcaggat caggacggcg tccaaagctg caatcggccg cgtc
//
LOCUS       DBNGWZ010000616        32635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1486058, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000616 DBNGWZ010000000
VERSION     DBNGWZ010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 32635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 32635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32635
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1486058"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC16CX_19035"
     CDS             complement(<1..322)
                     /locus_tag="AC16CX_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009970405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR3014637.1"
                     /translation="MPEFSTIAAVDLGSNSFHCQVGRIVGDQVYPLDTLREPVRLGGG
                     LTRDKRIDEEVQERALACLKRFGERLRDFDKHAVRAVGTNTLRVAKNAPAFLKKAEAA
                     LGFPI"
     gene            complement(570..1229)
                     /gene="rpiA"
                     /locus_tag="AC16CX_19040"
     CDS             complement(570..1229)
                     /gene="rpiA"
                     /locus_tag="AC16CX_19040"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR3014638.1"
                     /translation="MTQDEMKQAAAKAAIGYVPDDCVVGVGSGSTVNFFIAELGKIKH
                     RIEAAVASSEASAKRLKDLGIRVVDLNGVDEVPVYVDGADEVTRHLAMIKGGGGALTR
                     EKIAAAVAKKFVCIADSTKLVEVLGKFPLPVEVIPMARSYVARELRKLGGHPEWREKF
                     VTDNGNVVLDVHGLTILNPAELEGALNQIAGVVTNGLFARRGADVLLLGAGRGVETLK
                     R"
     gene            1335..2879
                     /gene="ilvA"
                     /locus_tag="AC16CX_19045"
     CDS             1335..2879
                     /gene="ilvA"
                     /locus_tag="AC16CX_19045"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR3014639.1"
                     /translation="MLRLAAFAHAMKKNDYLERILSARVYDVAIETPLEVAPALSART
                     GNRVLLKREDMQPVFSFKLRGAYNKMAHLPAAALRRGVIAASAGNHAQGVALAAQRLA
                     CRATIVMPVTTPQIKIAAVKARGATVILHGDSYDEAYVHARALGRRRGLAFVHPYDDP
                     DVIAGQGTIAMEILRQHPESIHAILVPIGGGGLISGIAAYVKRLRPEIKVIGVEPEDA
                     DAMYCSLEAGRRVKLAQVGLFADGVAVKQVGAETFRLCRALVDDVIRVNTDAICAAIK
                     DVFEDTRVVLEPAGALAIAGVKAYVGRTGVRGKTLVAIASGANMNFDRLRFVAEEAEL
                     GEKREAVLAVTIPERPGSFREFCSLIGARNITEFNYRYADPMRAHVFVGVQVHDRKET
                     DRLIRKLRRRGLKTLDFTDNELAKLHIRHTVGGHAPTVDDEILYRFEFPERPGALMKF
                     LTAMSRGWNISLFHYRNHGADYGRVLVGMQVPPRDKRKFRAFLAKVGYPFNEETRNPA
                     YRLFLG"
     gene            3199..5124
                     /locus_tag="AC16CX_19050"
     CDS             3199..5124
                     /locus_tag="AC16CX_19050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3014640.1"
                     /translation="MSRLWGALLVLVAVGAQGAALDDEFLAAREAFRIGDAVILEQHA
                     ARLKGYLLEQYVAYWQLSMRLEQASAEEVRAFLAANRDSPLSERLRNEWLKTLGKRGD
                     WELFDAELPLLVGDDIEITCYALQSRLRVNPDGTLPEARALWFVPRDLPESCTPLFNA
                     LAAAGMLSNDALWTRIRLALEAGQVSLARYIAEWLPAGQAPEARVLERIASNPAGYFE
                     RHQGFDLKSRAMRETVMFAVHRLARTSSQQAARHWARLEERFSQDERAYVWGMIGYLG
                     AMRHDRDALAWYARAGDLSDLQLAWKARAALRARDWGEVLAATEAMTGNESSQPAWRY
                     WKARALRQLERREEAVELLRPLATEFNFYGQLALEELGGKITVPAAPYKPGAEDVRAM
                     GKHVGLRRALTLYRLNLRTEANREWLWAIRGFDDKQLLAASEVARRHEVYDRAINTAD
                     RTVALHDFSLRYLAPYRDVLQPHTAELALDEAWVYGLIRQESRFIPDAKSRAGASGLM
                     QLMPGTARWVARKLGLKDWRWSQVTEVDTNVSLGTYYLRHVLDALDGYPLLASAAYNA
                     GPGRARAWRPEGAIEGAVYAETIPMNETRDYVKKVMASTSYYAHTFSQQVQSLKQRLG
                     IIGPRQRDKEASLGDTP"
     gene            5224..6213
                     /locus_tag="AC16CX_19055"
     CDS             5224..6213
                     /locus_tag="AC16CX_19055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR3014641.1"
                     /translation="MEITEVCVLGGSGFVGRHICHVLAAEGYRVRVATRDRERAKEKL
                     ILLPTVDVAVVNVHEPGQLAAFVRGADAVINLVGVLHDGRGERSFRSAHVELARKVVA
                     ACRESGIQRLLHMSALNADPNGPSAYLSTKGEAEALVRESRLAWTVLRPSVVFGREDS
                     FINPFARALRFLPVLVLPCPGARFQPVFVEDVAAVFVKSLADLGSVGKAYDLCGPKVY
                     TLRELVEYVGTATDRRRMIIGLNDTLSYFQALAMELFPVKQVMLAFDMLMTRDNYRSM
                     KVDSICRCDFPFSIAPTALEAVAPSWLGQSTPRARYQRFRGQARRNEPVNGES"
     gene            6323..7441
                     /locus_tag="AC16CX_19060"
     CDS             6323..7441
                     /locus_tag="AC16CX_19060"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HIR3014642.1"
                     /translation="MKIYCVGGAVRDELLRLPVKDRDYVVVGATPEDMVKQGYKAVGK
                     DFPVFLHPVTHEEYALARTERKTARGYHGFEFHAAPDVTLEQDLARRDLTINAIARDA
                     EGRLVDPYHGADDLKAGVLRHVSPAFAEDPVRILRVARFAARYGFRIARETMKLMRDM
                     AEEGEADALVPERVWQEIARGLMEAVPSRMFDALRQSGALARVMPEVDALWDDADTAQ
                     AAMRALDAAARAGATLPARFAALARSLDPLAVESLVGRLKVPADCRDLALLAARHANI
                     VLDAAELDPDSVLDLFNTVDAWRRPERFAELMAAAFAGEPDAAPAQARLERARAAAAA
                     VNAGEIAKASQGAAAIRSNVDAARLEAIRRALAEGASD"
     gene            7543..8169
                     /locus_tag="AC16CX_19065"
     CDS             7543..8169
                     /locus_tag="AC16CX_19065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR3014643.1"
                     /translation="MIDLYTWTTPNGRKVSIMLEELALPYRVHPINIGQGDQFKPEYV
                     KINPNSKIPSLVDPEGPDGKPISLMESGAILIYLAGKTGKLLPASVRGKYEALQWLMF
                     QMGGVGPMFGQVHHYLRSAKEQVPYAIERYTKETRRLYGVLNDRLKDRSYLADGYSVA
                     DIATYPWVARYEWHKTNLEDFPHVKRWFDAISARPAVQRGMNVPPVTK"
     gene            8342..8860
                     /locus_tag="AC16CX_19070"
     CDS             8342..8860
                     /locus_tag="AC16CX_19070"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR3014644.1"
                     /translation="MAIFATPVTALAQQCRVTPRDALGPFYKPNAPAQAELCASGSGG
                     SRKLLVTGRVLGAPDCAPIAGALVEVWQADARGDYSQVGAKRDDPGCLLRASLETDAE
                     GRYSFRTVAPGEYPGRPRHIHYRVSAKGYATLVTQLYFARERGIPPELVVTAVAKDGA
                     LAASFDVTLAKA"
     gene            9109..9513
                     /locus_tag="AC16CX_19075"
     CDS             9109..9513
                     /locus_tag="AC16CX_19075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014645.1"
                     /translation="MRRAIAARLMGEATNGAGRPILPALKEFLIHGVRYAYPPERGEL
                     TRGMPTGYAAPPLNKVIVQPNEPPPVWPYAEGKVRGYKFAPLFLTVPIAAARDPKLYE
                     LLALVDAIRDGRARERSLAAKELDLRLAAYGQ"
     gene            9513..10205
                     /locus_tag="AC16CX_19080"
     CDS             9513..10205
                     /locus_tag="AC16CX_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014646.1"
                     /translation="MADSNIELLMGVAEALGDLREQFVFVGGCATALLITDTAAPPVR
                     ATHDVDAIVAIVSLLDYHRLGGDLRARGFVQTLAEGEPPYRWTLAGMKLDVMPISETV
                     LGFSNRWYEPAMRTAASVQLRHGLEIRLVAPAYFLATKLAAFEGRGRGDYLESHDLED
                     VLSVVDGRPEIVDEITTAEAQLRSYVAATFARLIADDGFLNALPGLIIEGSPAIRSPV
                     VLQRLRMIAALK"
     gene            10504..10719
                     /locus_tag="AC16CX_19085"
     CDS             10504..10719
                     /locus_tag="AC16CX_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014647.1"
                     /translation="MTAISAELLAKELESLRQLTRGAMKKRISDKHGEKLVRCGFAKF
                     QTGALTITTMGHAKLAIEITRASWLPI"
     gene            10743..10925
                     /locus_tag="AC16CX_19090"
     CDS             10743..10925
                     /locus_tag="AC16CX_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014648.1"
                     /translation="MDEIVGFDELRWLRELTYKTQKAEMPEIIGTRLLTRGLIERKAG
                     GFALTARGRIALAKLG"
     gene            complement(11062..11131)
                     /locus_tag="AC16CX_19095"
                     /pseudo
     CDS             complement(11062..11131)
                     /locus_tag="AC16CX_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
     gene            complement(11142..19793)
                     /locus_tag="AC16CX_19100"
     CDS             complement(11142..19793)
                     /locus_tag="AC16CX_19100"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR3014649.1"
                     /translation="MAQKYAADEPPLRSELLSADQMEQHGRTLAGSHKLSLTRAPDRL
                     LTRLTENEAVLIGACNLLTAAVKANRRIAPAGEWLLDNFYLIEGQIRTAKRHLPKGYS
                     RELPRLQNGPSAGLPRVYDIALETISHGDGRVDSESLSGFVAAYQKVTALKLGELWAI
                     PIMLRLALVENLRRVGARIAANRIDRNGADYWADQMMEVAEKDPKSLILVIADMARSG
                     QPMVSSFVAEFARRLQGQSPALALPLTWIEQRLSEDGLTIEQLVRSENQQQAADQVSM
                     SNSIGSLRFLGAMDWHEFVETMSVVEQKLREDPGGVYGKMDFATRDRYRHVVEKIARS
                     SRLSESEVARKAIQLAHEGTARKDGDDRAAHVGFYLIDKGLPQLEQTAEVRLSAFEAL
                     QRMSCRFPLLLYLGTILLLTVVFTGSLLAKAHADGLLGWMLALIGILSLLCASHLAVA
                     LVNWLATLLATPHPLPRMDFSGGIPPESRTLAVVPTMLMSAQNIEDLIEALEVRFLAN
                     RDEHLHFGLLTDFRDAHEETLPEDEPLLLLAWKRIEELNEKYRSAKGNTFFLFHRPRR
                     WNPQERIWMGYERKRGKLAELNSLLRGGSTDRFSLVVGETAVLSNVKYVITLDTDTQL
                     PRDSAWEFVGAMAHPLNRARYDEHKQRVCEGYGILQPRMAVSLPGANRSRYARLCGSE
                     PGIDPYTRAVSDVYQDLFREGSFIGKGIYDVDAFERALKGRFPENRILSHDLLEGCYA
                     RAGLLSDVHLYEDYPSRYSADVSRRHRWIRGDWQIARWLLPHVPGSGASRQRNPLSDL
                     SQWKIFDNLRRSLVPLALTLLLLLGWTALSPVWFWTLSVIGIILIPPLLASILEMFQK
                     PDDVLLGQHLAAALRSAGRHFARAALTLVCLPYEAFFSLDAIVRTAGRMLVTHKRLLE
                     WNSSGELDRISRMDFIASCRSMWIAPAIASAAAIYLTQSNPAALAVAGPILCLWFAAP
                     AIAWWISRPLARRAARLTADQTLFLQKISRKTWAFFETFVGPEDHWLPPDNYQEHPVT
                     MVVHRTSPTNMGLALLANLSAYDFGYIPAGELIERTANALHTMEALERHQGHFYNWYD
                     TQSLQPLPPLYISSVDSGNLAGHLLTLRPGLLALPDHKILGSRLFEGLSDTLRILVDA
                     AGGAASPQLAQLQKDLESAYDSRPATVAAARRWLARLATSAAEVAGGLAAAPESEAKW
                     WAQALARQCRSALDELTFLAPWSLLPAAPSGLSDFPDIGEIPTLRELARLEVEWLPAI
                     GRRLGSDATPEESGWLDELRRLITEASRRAKERIAAIERLALQSSDLARMEYEFLFDK
                     TRHLLAIGYNVGERRRDSSYYDLLASEARLSSFVAIAQGQLPQESWFALGRLLTTAGG
                     EPILLSWSGSMFEYLMPLLVMPTYEHTLLDQTYRAAVKRQIAYGNQRGVPWGMSESGY
                     NTVNAGLNYQYRAFGVPGLGLNRGLADDLVIAPYASALALMVAPEEACLNLQRLAAEG
                     LEGKFGFYEAIDYTPSRLPRGQSSAVVRSFMAHHQGMIFLSLADLILDRPMQKRFESD
                     PLFQATTLLLQERIPKATAFYSHTAELSDIRAISSGPEMPVRVLSSPDTPIPEVQLLS
                     NGRYHVMVTNAGGGYSRWKDLAVTRWREDSTCDNWGTFCYIRDVTSGVFWSTAYQPAL
                     KRSEAYEAIFSEGRAEFRRSDHDIDTYTEIVVSPEDDIELRRLRITNRSRTSRTIDVT
                     SYVEVVLASSAADALHPAFSNLFVQTEIIRQRQAILCTRRPRSLEEQAPWMFHLMAVH
                     GADVGEVSYETDRMRFIGRGRTVAAPQAMSDSAALSGSQGSVLDPIVAIRYRITLDPE
                     QSATIDMVSGIGETRDACLGLVGKYQDRHLADRVFELAWTHSQVVLRQINATDADAQL
                     YGHLASSVIYANSSLRADAAVLSKNRRGQSGLWGYAISGDLPIVLLQIKDPANIELVR
                     QLVQAHAYWRLKGLAVDLVIWNEDHAGYRQLLHEQIMGLIAAGLEAHVMDRPGGIFVR
                     AAEQISDEDRILFQSVARAILTDSRGALAEQINRRGLVEVTVPRLTPTRTRRAEPSPG
                     AALPRHDLNFFNGLGGFTPDGREYVITTAHGQVTPAPWMNVLANPHFGTLVSESGQAY
                     TWSENAHEFRLTPWYNDPVSDSSGEAFFIRDEERGHFWSPAPLPSCGAMPYVSRHGFG
                     YSVFEHTERGVRSELWVYVALDAPIKFAVLKVRNESGRLRRLSATGYVEWVLGDLRPK
                     SALHVITKVDPNSGALFARNPYNTEFADRTTFFDVDDATRTFSGDRTEFLGRNGTLKN
                     PAAMTRSQLSNKVGAALDPCAAIQVPFELADGQEREIIFRLGVGRDADDASNLVHRFR
                     GSTAARGALDTVWQHWKHTLGAVQVETPDPSLNVLTNGWLLYQTIACRLWARSGYYQS
                     AGAFGFRDQLQDVMALIHAEPRLVREHLLLCASRQFKEGDVQHWWHSPSGRGVRTHCS
                     DDYLWLPLATCRYVSSTGDTGVLDEPVHFVEGRPVNAEEDSYYDLPVRSEEAASLYQH
                     CVRAILRGLTFGEHGLPLIGSGDWNDGMNIVGKHGKGESVWLGFFLYEVLMRFTGVAR
                     VHGDNSFAERCQREAAQVRQNIEQNGWDGEWYRRAYFDDGSPLGSASNPECQIDSIAQ
                     SWSVLSAAGDARRSRMAMEAVDKRLVCRDHALIQLLDPPFDKSTLNPGYIKGYVPGVR
                     ENGGQYTHGAIWAAMAFAALGDSRRAWELTAMINPVNHARSPEAIATYKVEPYVVAAD
                     VYALPPHTGHGGWTWYTGSAGWMYRLIVESLLGLKLEVDKLRFAPCLPADWETFKVHY
                     RYRETFYHIAVLHTRAGNGEMSVTVDGVEQHDKAIPLVDDRQEHSVEVRIHAARS"
     gene            complement(20279..20998)
                     /locus_tag="AC16CX_19105"
     CDS             complement(20279..20998)
                     /locus_tag="AC16CX_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3014650.1"
                     /translation="MPASHKPEQNQLLAALPAADYERLLPHLEPVTLPLGLVIFESGS
                     KLRHLYFPASGIVSLLYAMENGASTEIAVIGNEGVVGIALFMGGESTPSRAVVQSAGH
                     GYRLKAAVLKTEFDLGGPLQHLLLRYTQALIAQMTQTAVCNRHHAVDQQLCRWLLLSL
                     DRLASSELTMTQELIANMLGVRREGVTEAAGKLQEAGLIRYRRGHITVLDRPKLEKRV
                     CECYAVVKKEMNRLLPDKTAS"
     gene            21207..21746
                     /locus_tag="AC16CX_19110"
     CDS             21207..21746
                     /locus_tag="AC16CX_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014651.1"
                     /translation="MVTTAAAATFGCAQKPPATVAVIEDKVYTVTPDQVMVKAGIVTG
                     EVTELKVTERVEKGSGRIESPAKLTGKLKLTNSSTDQTVRLVGGRILYIDDQGQLIKI
                     EETRTEPALKFPTSYNATDRLDPGQDATQSVEVDFPAAALTAKKLKEIRIELVYIPSS
                     YKLQTANFSVSIGGQSVSQ"
     gene            21898..22056
                     /locus_tag="AC16CX_19115"
     CDS             21898..22056
                     /locus_tag="AC16CX_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014652.1"
                     /translation="MTSTEEQQMLASLSEISQDIKALLTLIKDLTRQQGDADEKAYGQ
                     LVNIAHKR"
     gene            complement(22081..22548)
                     /locus_tag="AC16CX_19120"
     CDS             complement(22081..22548)
                     /locus_tag="AC16CX_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014653.1"
                     /translation="MTEELTSKLEKALDQFTRTRAPGADRKPQAKTSKDDFPGDFAKL
                     KLSVIRPAFEAIGNLLKERGDEINISEDPGGKISIHIVPAGVKKSIHPYDWYPTLSFF
                     GAPFTRTIGLHGRNARRNSDSGSRPRGDYKLAQVNKEIVEKELMKFVGEIANW"
     gene            complement(22596..23345)
                     /locus_tag="AC16CX_19125"
     CDS             complement(22596..23345)
                     /locus_tag="AC16CX_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3014654.1"
                     /translation="MPAAIPIPVANSLLAALPRAEYQRLLAGLEPVELAFGEVLYQPG
                     EPIRHTYFPNDCLVSLLAVVEGRTVLEVGLVGSEGMVGIPLALGIGVSPIRALVQRTG
                     TAMRMESARFGREFSRNPSLRRDLFHYTHELMAQATQTAVCSRFHLLEARLARSLLMT
                     RDRMQSNDFRLTHEFLAHMLGVRRVGVTKAAGTLQQRKLISYSRGGIRILDGKGLEAA
                     SCRCYGIVKDMHDLTLREYSNRKNSPIAAVA"
     gene            23580..24167
                     /locus_tag="AC16CX_19130"
     CDS             23580..24167
                     /locus_tag="AC16CX_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HIR3014655.1"
                     /translation="METQANKSPHMLFWIAGIAVTVFSAVGIAAIMGWIPTSTSSPSE
                     NIALAKQPTAPAKTAAARARATPEQAPVQVASNKPVVRAVCAECGVIESVREIDTKGE
                     GSGIGAVGGAVVGGVLGHQVGSGRGQDVATVVGAVGGAVAGNEIEKRVKSGKSYEITV
                     RFEDGSSRVINEANAPSWRAGDRVKVINGVIQSNA"
     gene            24205..24354
                     /locus_tag="AC16CX_19135"
     CDS             24205..24354
                     /locus_tag="AC16CX_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HIR3014656.1"
                     /translation="MLYTIAVVLLVMWLLGLVTSYTIGGFIHVLLVIAIVVVLLRIIG
                     GRKPV"
     gene            24392..24526
                     /locus_tag="AC16CX_19140"
     CDS             24392..24526
                     /locus_tag="AC16CX_19140"
                     /inference="COORDINATES: protein motif:HMM:NF019696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR3014657.1"
                     /translation="MFQRILATLILLGMLGAIAGCNTMQGFGEDMERAGEKIQQKAAR
                     "
     gene            24557..24943
                     /locus_tag="AC16CX_19145"
     CDS             24557..24943
                     /locus_tag="AC16CX_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014658.1"
                     /translation="MGIIPTTAVPASDEAIDAIAGIEMQRQVMEKEAGLQASCSSALR
                     KLHELLDREKEALQLHGAGTGHPENVEAVMVEIERVKGLAVITSQGPAPRSAYYPGQR
                     KASWRNAHRSPARNKGRRTMGRAGGR"
     gene            24966..26024
                     /locus_tag="AC16CX_19150"
     CDS             24966..26024
                     /locus_tag="AC16CX_19150"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DesA family fatty acid desaturase"
                     /protein_id="HIR3014659.1"
                     /translation="MDLFFSGVFDLPWWGHVIVALVLTHITIVAVTVFLHRHQAHRAL
                     DLHPAVSHFFRLWLWLTTGMVTREWAAIHRKHHARVETPEDPHSPQVLGINRVLWGGV
                     FLYVTESRNRETIERYGQGTPDDWLEHNVYSRYVKTGLTLMGLADVILVGIVPGALIF
                     LVQIVWIPFWAAGVINGIGHFRGYRNWPTEDASTNIVPWGILIGGEELHNNHHAFTTS
                     AKLSSKWYEFDIGWMYIRILETLGLAKVKRVAPVPRFAAPRLIPDLQTLQAVITHRYD
                     VLAKYAKSLQRIGAGEQHAHALQTMHAMRRELMALWERSTASKEQLVRRLQNWCQRAE
                     TSGIQPLVEFSKRLRCYA"
     gene            26051..26344
                     /gene="groES"
                     /locus_tag="AC16CX_19155"
     CDS             26051..26344
                     /gene="groES"
                     /locus_tag="AC16CX_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3014660.1"
                     /translation="MKIRPLHDRVIVKRLEEERKTASGIVIPDTAAEKPDQGEIMAVG
                     KGKILENGKVRPLDVKVGDKILFGKYSGQTVRVKGDELLVMREEDIMGVVEGR"
     gene            26382..28022
                     /gene="groL"
                     /locus_tag="AC16CX_19160"
     CDS             26382..28022
                     /gene="groL"
                     /locus_tag="AC16CX_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753915.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3014661.1"
                     /translation="MSAKDVRFHDSARNKMVEGVNILADAVKLTLGPRGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKNRFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVQE
                     GMKYVAAGMNPMDLKRGIDQAVVATVDELRKLSRPCTTNKEIAQVGAISANADEAIGK
                     LVADAMDKVGKEGVITVEDGKGLDNELEFVEGMQFDRGYLSPYFINKPDKQSAVLEDA
                     YILFHDKKISAIRELLPVLEQVAKAGKPLLIVAEEVESEALATLVVNGIRGILKTCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEELGLKLENVTLKDLGRCKRVEVEKENTTLIG
                     SAGEKKAIEGRIKSIRGQIEDTSSDYDREKLQERLAKLAGGVALIRVGAATELEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRCREAVARIKGDNHDQNSGIKIVLRALEEP
                     LRQIVANTGDEPSVVLNKVAEGKGNFGYNAVTGEYGDLVKMGVLDPTKVTRYALQNAA
                     SVAGLILTTDAMVADLPKDDLNGGHGMGGMGGMGGMDM"
     gene            28042..28425
                     /locus_tag="AC16CX_19165"
     CDS             28042..28425
                     /locus_tag="AC16CX_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014662.1"
                     /translation="MQWWGYSKEHGWVVLDRRIPSNAPGLKADLLFLRCRDAMIFGAK
                     RESWNPPLYRFAPNYIRDLAPPASDEAAAELEGFKVRWPEFERELQRECRETEERAQA
                     VRDQEEKIRKQVASEKKKQAAAAKA"
     gene            28588..29010
                     /locus_tag="AC16CX_19170"
     CDS             28588..29010
                     /locus_tag="AC16CX_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR3014663.1"
                     /translation="MTSRLKAENREVAAPLALEAEAARNVRWRTDPADCLTYRCADWL
                     YLLCEGKMNIAMWVLAGGILGWIGFAYLKVNEDRGMVISIIIGVLGGFFGGNVLAPML
                     GAVADTPNDFSLFAMAVALASAAGCLAIGNLVSNRYGV"
     gene            29056..29391
                     /locus_tag="AC16CX_19175"
     CDS             29056..29391
                     /locus_tag="AC16CX_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014664.1"
                     /translation="MTELLSAVRFQEELRRVARFRSRIQVGDPLDAAVRKITQNPAFT
                     QSRLLARILAALTYQEGEFRRAEIAAFDADTLAMVITLMDAHAAGTSAREEWIGAVEA
                     TRNALLGVQ"
     gene            complement(29476..30717)
                     /locus_tag="AC16CX_19180"
     CDS             complement(29476..30717)
                     /locus_tag="AC16CX_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR3014665.1"
                     /translation="MTGTRIAHNLKLLARHDADGAPNLGEGMAMKLKDGRRYLYIAHE
                     HGPACFSIFDVTEPREPKLLWQLPLPHNNVRGNSLALRGDILLQAYQVNKPGLKPAGF
                     QVYDVSKPAQPREITFFDLSGPPNSHAHGVHYVSFMDGRYAHITAGHPDFVPNDPKDH
                     QFYMIVDLANPSQPVEAGRWWLPGQRKGDREPAPTRHVPPSFDFAFRPHHTLCYPERP
                     DRAYIGYIDAGIVILGISDKARPKLISRLDYHPPLPGFTHTVLPLFDRDLLVVTDEAT
                     GDHGLDWPKRCWIVDARLESNPVMISSLPTPRDFGELHKVGGRIGAHNLHENETEPGA
                     ARLQNTVVSTWFSAGLRVYDISDPYRPEEIAVFLPETPEGQRGCRISDVFVDDRGIIY
                     AADRARGGLYVLEYTGKRPLN"
     gene            complement(30898..31212)
                     /locus_tag="AC16CX_19185"
     CDS             complement(30898..31212)
                     /locus_tag="AC16CX_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HIR3014666.1"
                     /translation="MAKLWQVAKLIRSKNSGPFELTFDVIFKDRATYEKVRDAKVINP
                     QWFAKTYRLTPEVVSVIEYDAAAAIKVTIPRPVISGDVDDTDVFGGQQYGPLVELDVP
                     EG"
     gene            complement(31289..>32635)
                     /locus_tag="AC16CX_19190"
     CDS             complement(31289..>32635)
                     /locus_tag="AC16CX_19190"
                     /inference="COORDINATES: protein motif:HMM:NF018938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR3014667.1"
                     /translation="SATGILGSGFREETLKRAMALKPDFIGADSGSTDPGPYYLGSGE
                     TLFSDSAYKRDLRLILLAARAAKIPAIVGSACTAGTDGQLEHLAGIAREIAREEKLSF
                     KLATIASEQDKSYLKRRLRERKITPLANAPEINEAVIDRSAHIVGMCGIEPYVEALER
                     GADVIIAGRSSDTSIFAALPVMRGFNPATVWHMAKILECGAACVVLRKYPDCMFATVT
                     DGSFVVEPPNPDYRCDPVSVASHNLYENSTPYELIEPSGVLNTVHAKYEAVSDRAVRV
                     SGSTFKPAERYTIKLEGAELAGYQSLIVGSVRDPVILRQFDSWLDGLVKAARDRIRAV
                     FGQDIESRYRFDVRVFGKNAAMGPLEPETGIGHEVGLLFQVTAQTQELATALMKSVGH
                     IAVHYPVPEWSGLITSIAYPYSPPEIARGASYRFNLNHVVEPASPLEMFHIGYENI"
BASE COUNT         6234 a        10132 c        10352 g         5917 t
ORIGIN      
        1 cgatcgggaa gccgagcgca gcctcggcct tcttgaggaa cgccggggcg ttcttcgcca
       61 cccgcagcgt attggtgccg acggcgcgca cggcgtgctt gtcgaaatcg cgcagccgct
      121 cgccgaagcg cttgaggcac gcgagcgccc gctcctggac ttcctcgtcg atgcgcttgt
      181 cccgggtgag cccgccgccc agacgcacgg gctcgcgcag cgtgtcgagc ggataaacct
      241 gatcgccgac gatgcgcccg acctggcaat ggaagctgtt tgaacctaag tcaacggcag
      301 caatcgtcga aaattcgggc attgcgctac caggaggtga gacggcggcc gcgcgcatgg
      361 cgcggcgcgt ggagggggaa gaatgccgcc gtggcgtgcg atcgcctgca gcgttgaact
      421 atcgactgac tggccgcaat cggttgcgtg gcgcgccctc tcgatgatcg atgcaagtat
      481 acggaatcct gcgccgcggc gccacgttag gcgggtcttt tcgtgcaatt ttgtataagg
      541 aaattgtcgg acttcaggcc ggcaatttcc taccgcttga gggtttcaac gccgcgcccg
      601 gcgccgagca gcagcacatc cgcgccgcgc cgcgcgaaca gaccgttggt gaccacaccc
      661 gcgatctggt tgagcgcgcc ctcgagctcg gcggggttca ggatcgtaag cccgtgcacg
      721 tcgaggacca cgttgccgtt gtcggtgacg aacttctcgc gccactccgg gtgaccgccc
      781 agcttgcgca actcgcgcgc gacgtaggat cgcgccatcg ggatcacctc caccggcagg
      841 ggaaacttcc cgagcacctc cacgagcttc gtgctatccg cgatgcaaac gaactttttc
      901 gcgaccgccg ccgcaatctt ctcccgcgtc agggccccgc cgccgccttt gatcatggca
      961 aggtggcgcg tcacctcgtc cgcgccgtcc acgtataccg gcacctcgtc cacgccgttc
     1021 aaatccacca cccggatacc gagatccttg aggcgctttg cgcttgcttc cgagcttgcg
     1081 accgcggcct cgatgcgatg cttgattttg cccagctcgg cgatgaagaa attaacggtg
     1141 gaaccgctgc cgacgcccac cacgcaatcg tcgggaacgt agccgatcgc ggcctttgct
     1201 gcggcctgct tcatttcgtc ctgcgtcatg accagaatag taagcggtaa gccgtaagcg
     1261 gtaaacagca tgtttcctcg caatgcgtca ttggcaccgc tcaccgcttg ccgctcacgg
     1321 cttacccggt ctctatgcta cgcttagcag ccttcgctca cgccatgaag aagaacgact
     1381 acctcgaacg catcctcagc gcgcgggtct acgacgtcgc catcgagacg ccgctcgaag
     1441 tcgcgcccgc gctctccgcg cgcaccggca accgcgtgct gctcaagcgc gaggacatgc
     1501 agccggtgtt ctccttcaag ctgcgcgggg cctacaacaa gatggcgcac ctcccggccg
     1561 ccgcgctgag gcgcggcgtg atcgccgcgt ccgcgggcaa ccacgcgcag ggcgtcgcgc
     1621 tcgcggcgca gcggctcgcc tgccgcgcca ccatcgtgat gccggtcacc acgccgcaaa
     1681 tcaagatcgc cgcggtcaag gcgcgcggcg caaccgtcat cctgcatggc gactcctacg
     1741 acgaggccta cgtccacgcg cgcgcgctcg gccggcggcg gggcctcgct ttcgtgcatc
     1801 cctacgacga cccggacgtg atcgccggcc agggcacgat cgcgatggag atcctgcgtc
     1861 agcatcccga gtccatccac gccatcctgg tcccgatcgg cggcggcggc ctgatttcag
     1921 gcatcgccgc gtacgtgaag cgcttgcgcc ccgaaatcaa ggtgatcggc gtggagccgg
     1981 aggacgccga cgccatgtat tgctcgctcg aagccggccg gcgcgtgaag ctcgcccagg
     2041 tggggctgtt cgccgacggc gtggcggtga agcaggtcgg cgccgaaact ttccggctgt
     2101 gtcgcgcgct ggtggacgac gtgatacggg tcaacaccga cgccatctgc gccgcgatca
     2161 aggacgtgtt cgaggacacg cgcgtggtgc tggagccggc cggggcgctg gcgatcgccg
     2221 gcgtcaaggc ctatgtcggg cggaccggcg tccgcgggaa aacgctggtg gcgatcgcca
     2281 gcggcgcgaa catgaacttc gaccgcctgc gctttgtcgc ggaggaagcc gagctcggcg
     2341 agaagcgcga agccgtcctt gccgtcacca tccccgagcg ccccggcagc ttccgcgagt
     2401 tctgctcgct gatcggcgcg cgcaacatca ccgagttcaa ctaccgctac gccgacccca
     2461 tgcgggcgca cgtgttcgtc ggcgtgcagg tccatgatcg caaggaaacc gaccggctga
     2521 tccgcaagct ccggcggcgc ggactcaaga cgctcgattt caccgacaac gagctggcga
     2581 agctgcacat ccggcacacg gtgggcgggc acgcgcccac cgtggatgac gagatcctct
     2641 accgcttcga gtttccggag cggccgggcg cgctgatgaa attcctcacc gccatgagcc
     2701 gcggctggaa catcagcctg ttccattacc gcaatcacgg cgccgattac gggcgtgtgc
     2761 tggtcggcat gcaggtgccg ccgcgggaca agcgcaagtt ccgcgccttc ctcgccaagg
     2821 tcggctatcc cttcaacgaa gaaacccgca atcccgccta tcgcctcttt ctaggctaac
     2881 gcgggtgatg gacacggcct tgcgccccgg gatgcgaaag gcgtgacaaa tgacgagtga
     2941 cgggcgtgga gtgtcgggtg tcgaggggcg agtgacgagt gtcgggtgtc aagtggaaac
     3001 acttgcttcc cgtcatagct ttttccctcg atactcattt ctcgacactc ttccgtcgac
     3061 actcaatcct cgacaccttt ccctcatccc tcatccctcc cccggcccgt agcgtcaact
     3121 gaacggccgg cccgggcgtt ctatctcctg aaaaaccggc tagaattaac gccataaaca
     3181 agggatttcg cgttgtatat gagcaggctg tggggtgctt tgctggtact ggtcgcggtg
     3241 ggcgcgcaag gcgccgcgct ggatgacgaa ttcctcgctg cgcgcgaagc gttccgcatc
     3301 ggcgatgccg tcatactcga gcaacacgcg gcccggctga agggttacct gctcgagcag
     3361 tatgtcgcct actggcagct gagcatgagg ctcgagcagg catccgctga ggaagtgcgc
     3421 gcttttctcg ccgccaaccg cgacagcccg ctctcggagc ggctgcgcaa cgagtggctc
     3481 aaaacgctcg gcaagcgcgg tgactgggaa ctgttcgacg ccgagctgcc gctgctcgtc
     3541 ggcgacgaca tcgagatcac ctgctacgcg ctgcagtcac ggttgcgcgt caatccggac
     3601 ggaaccctgc ccgaggcgcg agcgctgtgg ttcgtgcccc gcgacctgcc cgaaagctgc
     3661 acgccgctgt tcaacgcgct cgccgcggcc ggcatgctgt cgaacgatgc cctgtggacg
     3721 cggatccggc ttgcgctgga ggccggtcag gtgagcctgg cgcgttatat agccgaatgg
     3781 ctgcccgcag gccaggcgcc cgaggcgcgc gtgctcgagc gcatcgcgtc caaccccgcg
     3841 gggtacttcg agcgtcacca gggattcgac ctcaaaagcc gcgccatgcg cgaaaccgtc
     3901 atgttcgccg tccaccgtct cgcgcgcaca tcgtcccagc aggcggcccg ccactgggcg
     3961 aggctcgagg aacgcttctc gcaggacgag cgcgcttacg tatgggggat gatcggctat
     4021 ctgggcgcga tgcgacacga ccgggatgcg ctcgcctggt acgcacgcgc cggcgatctt
     4081 tccgacctgc agctcgcctg gaaggcgcgc gccgcgctgc gcgcccgcga ctggggggaa
     4141 gtgctggcgg cgaccgaggc catgacgggc aacgagtcct cgcagcccgc ctggcgctac
     4201 tggaaggcgc gcgcgttgag gcagctggaa cgccgcgagg aggcggtgga attgctcagg
     4261 ccgctcgcga ccgagttcaa tttctacggc cagctagcgc tggaggagct gggcggcaag
     4321 atcacggtac ccgccgcgcc ctacaagccg ggcgcggagg acgtgcgcgc gatgggcaag
     4381 catgtcggcc tgcggcgcgc gctcacgctc taccgcctga acttgcgcac cgaggcgaac
     4441 cgggaatggc tgtgggcgat ccgcggcttc gacgacaagc agctgcttgc cgcctccgaa
     4501 gtcgcgcgcc gccacgaggt ctacgaccgc gccatcaaca ccgcagaccg caccgtggct
     4561 ctgcacgatt tcagcttgcg ctacctcgct ccctatcgcg acgtgctcca gccacacacc
     4621 gccgagcttg ccctggacga ggcgtgggtc tacgggctga tccggcagga gagccgtttc
     4681 atcccggatg ccaagtcgcg cgccggcgcg agcggcctga tgcagctcat gcccggcacc
     4741 gcgcgctggg tggcgaggaa gctggggctg aaggactggc gctggtcgca ggtcaccgag
     4801 gtggacacca acgtgagcct cggcacctac tacctgcggc acgtgctcga tgcgctcgac
     4861 ggttatccac tgctcgcttc cgccgcctac aacgccggcc cgggccgggc gcgcgcctgg
     4921 cgtccggagg gcgcgatcga aggcgcagtc tacgccgaga ccattccaat gaacgagacc
     4981 cgcgattacg tgaagaaggt catggcgagc acgagctact atgcccacac cttcagccag
     5041 caggtgcagt cgctcaagca gcgcctcggc atcatcggcc cacgccagcg cgacaaggag
     5101 gcgtcgctgg gagacacccc ctaggatttt gccggcccaa ggtccgacaa aatcctgaag
     5161 cggagctcac tgacgggagg gaactgtcac cgaaggcaga aggcccgggg gtaaaatctc
     5221 tccatggaga tcactgaggt ttgcgttctg ggcggctccg ggtttgtcgg gcggcacatc
     5281 tgccacgtgc tcgcggcgga agggtaccgg gtgcgggtcg cgacgcgcga ccgcgagcgc
     5341 gccaaggaaa aattgatcct cctgccgacg gtggacgtcg ccgtcgtcaa cgtccacgag
     5401 ccggggcaac tcgcggcatt cgtgcgcggg gcggacgcgg tgatcaatct ggtgggcgtg
     5461 ctgcacgacg gtcgcggcga gcggagtttc cgctcggcgc acgtggagct cgcgcggaag
     5521 gtcgtcgcgg cgtgccgcga gagcggcatt cagcgcctgc tgcacatgag cgcgctcaac
     5581 gccgatccca atggcccgag cgcctatcta agcaccaaag gcgaggccga agcgctggtg
     5641 cgcgagtcga ggctggcgtg gaccgtattg cggccttcgg tcgtattcgg gcgcgaggac
     5701 agttttatca atccgttcgc gcgggctctc agattcctgc cggtgctggt gctgccctgt
     5761 ccgggcgcgc gcttccagcc ggtgttcgtt gaggacgtag ccgcggtttt cgtgaagagc
     5821 ctcgctgacc taggcagcgt gggcaaggct tatgacctgt gcggtccgaa ggtctatacc
     5881 ttgcgggagc tggtcgagta cgtgggcaca gcgacagacc ggcggcggat gatcatcggt
     5941 ctcaacgata cgctttccta cttccaggca ctggcgatgg agctcttccc ggtgaagcag
     6001 gtcatgctcg cgttcgatat gctgatgacc cgcgataatt accgctcgat gaaggtggac
     6061 agcatatgcc gctgcgactt tccgttcagc atcgcgccga ccgcgctgga agcggtggcc
     6121 ccgtcctggc tcggccagtc cacgccgcgc gcgcgctacc agcgcttccg cgggcaggca
     6181 cgccgcaatg aacccgtgaa tggtgaatcg tgattcgtga ttcgtgcggg agggaaaagg
     6241 cgcgaatcac ggctcacgaa tcccgactca cgcttttctg cagggatcga cccacgaccc
     6301 acgatccacg acccacggtc ttatgaagat ctactgcgtc ggcggagcgg tgcgggacga
     6361 gctgctgagg ctgccggtca aggaccgcga ttacgtcgtc gtcggggcga cccccgagga
     6421 catggtgaag cagggctaca aggcggtcgg caaggacttc ccggtcttcc ttcaccctgt
     6481 cacgcacgag gagtacgccc tggcgcgcac ggagcgcaag acggcgcgcg gctaccacgg
     6541 cttcgagttc catgccgcgc ccgacgtcac gctcgagcag gacctggcgc ggcgcgatct
     6601 caccatcaat gcgatcgcca gggacgcgga ggggcgcctc gtggacccgt accacggcgc
     6661 cgacgatctc aaggccggcg tgctgcgcca cgtcagtccc gccttcgccg aggacccggt
     6721 caggatattg cgcgttgcgc gcttcgccgc gcgctacggt ttccgcatcg cccgggaaac
     6781 catgaaactg atgcgcgaca tggcggaaga aggggaagcc gatgcgctgg ttcccgagcg
     6841 cgtgtggcag gagattgcgc gcgggttgat ggaggccgtg ccgtcgcgca tgttcgacgc
     6901 cctgcgccag agcggcgcgc tggcgcgcgt gatgccggaa gtggacgcgt tgtgggacga
     6961 tgccgacacc gcgcaggccg cgatgcgcgc gctcgacgcg gcggcgcggg cgggcgcgac
     7021 gctgcccgcg cggttcgccg cgctcgcgcg ctcgctcgac ccgctcgccg tcgagtcgct
     7081 cgttggccgg ctcaaggtgc ccgccgactg ccgcgatctc gcgctgctcg cggcgcgcca
     7141 cgccaacatc gtgctcgatg ccgcggagct ggacccggat tcggtgctcg acctgttcaa
     7201 caccgtggac gcctggcgca ggccggagcg cttcgcggag ctgatggcgg cggcgttcgc
     7261 cggcgaaccc gacgccgccc ccgcgcaggc gcggctcgag cgcgcgcggg ccgcggcggc
     7321 cgcggtcaac gcgggggaaa tcgcgaaggc ctcgcaaggc gccgccgcca tccgcagcaa
     7381 cgtggacgcg gcgcggctgg aagccatacg ccgcgccctc gccgagggcg cgagtgacta
     7441 agtgatttag tgattgagtg attcagtcac agcaattacc cggcgtcttg cgcacctaat
     7501 cacttaatca cctaatcact caatcaccca caagggaatg aaatgattga tctctataca
     7561 tggaccacgc ccaacggccg caaggtctcg atcatgctgg aggagctggc gctcccatac
     7621 cgcgtgcacc cgatcaacat cggccagggc gaccagttca agccggagta cgtgaagatc
     7681 aaccccaaca gcaagatccc gtccctcgtc gacccggaag gccccgacgg caagccgatc
     7741 tcgctgatgg aatccggcgc gatcctcatc tacctcgcgg gcaagaccgg caagctgctg
     7801 ccggcctcgg tgcggggcaa gtacgaagcg ctgcaatggc tcatgttcca gatggggggc
     7861 gtcgggccga tgttcggcca ggtgcaccac tacctgcgct ccgcgaagga gcaggtcccg
     7921 tacgcgatcg agcgctacac caaggagacc cgccgtctct acggcgtgct gaacgaccgc
     7981 ctcaaggacc ggtcttatct cgccgacggc tactcggttg cggacatcgc gacctacccg
     8041 tgggtcgcgc gctacgagtg gcacaagacg aatctggagg acttcccgca cgtcaagcgc
     8101 tggttcgacg cgatctcggc gcgcccggcg gtgcagcgcg gcatgaacgt gccgccggtg
     8161 acgaaatgac gcggtgatga agtgacgaaa agctgaaccg ccaagacgcc aggagaagca
     8221 gcgacacaac aattgaaccg ccaagtcgcc aaggacgcca aggacgccaa ggatactgat
     8281 aaagatgatg actacgatgg caaagcctga ttcccggcga cgaaaggtaa ttactgcggg
     8341 cttggcgatc ttcgcgacgc cggtgacggc gctcgcgcag cagtgccgcg ttacgccgcg
     8401 cgatgcactc gggccgttct acaagcccaa tgcgcccgcg caggcggagt tgtgcgcaag
     8461 cggctccggc gggagccgga agctgctggt cacgggccgc gtgctgggcg cgcccgactg
     8521 cgcgccgatc gcgggtgcgc tcgtggaggt atggcaggcg gacgcacgcg gcgactactc
     8581 gcaggtcggc gcgaagcggg acgaccccgg ctgcctgctg cgcgcgagcc tcgagacgga
     8641 cgccgaagga cgctacagct tccgcaccgt ggccccgggc gagtacccgg gccggccgcg
     8701 ccatatccac taccgcgtga gcgcgaaggg ttacgcaacg ctcgtgacgc agctctattt
     8761 cgcgcgcgag cggggcatac ccccggaact cgtcgtgacc gccgtggcga aggacggcgc
     8821 gctcgcggcg agcttcgacg tgacgctcgc gaaggcgtag ttctatttag ccgccgatga
     8881 acgccgatag acgccgatga aacatgaacc ttacgctagt ggcagcgcag cttgagggat
     8941 agaggcaggc ttgcgctatt cgcgattcgc gaatagaata agtccatgat tctaaagcct
     9001 caggatgtcg tcattctgct caagctggtc gccttgggcc ctcagccttg gacgtaccag
     9061 cggctcgcct cagagctttg gatcagccag tcggaggctc acgcgggggt gcgacgcgcg
     9121 atcgccgcgc ggttgatggg cgaggcgacc aatggggccg gccggccgat tcttccggcg
     9181 ctaaaagaat tcctcataca tggtgttcgt tatgcttacc cgcccgagcg cggagagcta
     9241 actcgaggca tgccgacggg gtacgcggcg ccgccgctta acaaggtgat cgttcagcct
     9301 aacgagccgc cgccggtgtg gccgtatgct gaggggaaag tgcgcggcta taagttcgcg
     9361 ccgttattcc taacggttcc gatagcagcc gcgcgcgatc cgaaactcta tgagctgctt
     9421 gcgctggtgg atgcgatccg agacgggcgc gcgcgcgagc gtagtctggc ggcgaaagaa
     9481 ctcgatttgc gcctggcggc ttacgggcag taatggccga ttcgaatatc gaattgctca
     9541 tgggggtcgc cgaggcgctc ggtgatttac gtgagcaatt tgtcttcgtg ggcggctgcg
     9601 cgacagcgct attgattacc gatacggcgg caccacccgt ccgcgcgacg catgacgtgg
     9661 atgcaatcgt ggcgattgtg tcgctgctgg actaccatcg cttgggaggc gatttgcgtg
     9721 ctcggggttt cgtgcagacg cttgcggaag gcgaaccccc atatcgctgg acgcttgcgg
     9781 gtatgaagct cgacgtaatg ccgatcagcg agactgttct cggattctcg aaccgctggt
     9841 acgagcccgc gatgcgaact gctgcaagcg tgcagcttcg gcatgggctg gagattcgtc
     9901 tagtagctcc ggcctatttt ctcgccacca aactcgcggc gtttgaaggt cgaggacgcg
     9961 gcgattacct cgaaagtcat gacctggagg acgtgctttc ggtcgtggac ggacgacctg
    10021 agatcgtgga tgaaattacg acggcggaag cacagctccg cagctatgtc gcggcgacat
    10081 ttgcgcgcct cattgccgac gacggattct taaatgcact gcctggcctc atcatcgagg
    10141 gcagtcccgc aatccggtcg ccagttgtgc tacagcggct gagaatgatt gcagcattga
    10201 aataggcctc ggagatttcc tgtttccgga aatacaatga ctcgaattac gacccacgaa
    10261 tcacgattca ccctttctcc ctcatccttc atcccctacc ctctggacgc cccgtcaccg
    10321 tcacgggttg acggaacatc gtgattgacc cttgcgccgc aaccgcgtat aagagaacga
    10381 ggcgtttagt ttcctctcct tgggcccgtt tcttccgggc cgctcctccg gccgcatttt
    10441 ttcggggctt gagattaaac attcccgggc ttgcctccgt ccgccgtccc atcaaggacc
    10501 cttatgactg ctatcagtgc agaattgctg gccaaagagc tggaatcgct gcgtcaactc
    10561 acccgtggag cgatgaagaa gcgcatctca gacaagcacg gcgaaaagct ggttcgctgc
    10621 ggctttgcga aatttcaaac cggcgcgctg actatcacga ccatgggcca cgcgaaactg
    10681 gcgattgaaa tcacccgcgc gagctggctt ccgatttgaa ttgacctgga acagcgtcgg
    10741 ccgtggatga gatagtcggt ttcgacgagc tgcgctggct gcgagagctg acatacaaga
    10801 cgcagaaagc ggagatgccc gagatcatcg ggaccaggct cctcacgagg gggctcatcg
    10861 agcggaaagc cggcggcttc gcgctcacgg caaggggccg gatcgcactg gccaagttag
    10921 gttagcggcc cggcccgagg atcacgcgca cccggtgcgc gccaccatca tccatcagcg
    10981 gaactcgggt cgggccgtcc ggcagcgtac tgccgtccag cgtggcgtag gcgactccac
    11041 ggctgacgcc atggggattc tcgaccgcaa tctccgccga cgccccgtct tccatgacgt
    11101 agagcagaga cacgatgctg gtggtgggga aatcttcgat atcaactgcg tgcggcgtgt
    11161 attctcacct cgaccgagtg ttcctgacgg tcgtcaacaa ggggaattgc cttgtcgtgt
    11221 tgctcaacgc catcaaccgt cacactcatc tcgccgttcc cggcacgcgt atgcagcacg
    11281 gcgatatggt agaaagtttc ccggtatcgg taatgcacct tgaacgtctc ccaatccgca
    11341 gggaggcacg gcgcaaaacg cagtttgtcg acttcaagct tcagccctag cagtgattcc
    11401 acgataagcc gatacatcca gccggcagat ccggtgtacc atgtccatcc gccgtggccg
    11461 gtatgcggcg ggagcgcata tacgtcggcc gcgacgacgt acggttctac tttgtaggtg
    11521 gcaatcgcct ccggagatct cgcatggttt accgggttga tcatggccgt gagttcccac
    11581 gcgcgccggc tgtcgcccaa tgcagcgaac gccatcgccg cccagatcgc cccatgagtg
    11641 tattgcccgc cattctctct cacgcccggg acgtagcctt ttatataacc ggggttcaaa
    11701 gtcgacttgt cgaacggcgg atccaaaagc tggatcagcg catgatcccg gcaaacgagg
    11761 cgcttatcca ccgcttccat cgccatgcgc gagcgtctcg catcccccgc agcggataga
    11821 acagaccagc tttgcgcaat cgaatcgatc tggcattcgg gattgctcgc cgacccaagc
    11881 ggcgagccgt cgtcaaagta ggcacggcgg taccactcgc catcccagcc attctgctcg
    11941 atgttctgtc gtacttgagc cgcctcccgc tggcaacgct cggcgaagga gttgtcacca
    12001 tgcacgcggg caaccccggt gaatcgcatg agcacttcat aaaggaaaaa gcccagccag
    12061 acgctttccc ctttgccgtg tttgcccaca atattcatgc cgtcgttcca gtcaccggag
    12121 ccgatgagtg gcaggccgtg ctcgccaaat gtgaggcccc tcaggatggc tcgcacgcag
    12181 tgttggtaca aactggctgc ttcttcggac cgtactggca gatcgtaata tgagtcctcc
    12241 tccgcgttta ctgggcggcc ctccacgaag tgaacgggtt catccagtac tcccgtgtcc
    12301 ccagtgctcg aaacgtaacg gcatgtcgcc aacggcagcc agaggtaatc atccgagcag
    12361 tgtgtacgta cgccacggcc cgacggagaa tgccaccaat gctggacatc cccttccttg
    12421 aattgacggg acgcacagag cagtagatgt tcgcgtacca ggcgcggctc ggcatggata
    12481 agcgccatca cgtcctgcaa ctgatcgcgg aaaccaaagg cgcccgccga ttggtagtat
    12541 ccgctccgcg cccaaaggcg gcacgctatc gtttggtaca gcaaccagcc attggtcagc
    12601 acgttgaggg acgggtcggg tgtttccacc tgcactgcgc cgagcgtgtg cttccagtgc
    12661 tgccacaccg tgtcgagagc gccgcgcgcg gcagttgatc cccggaagcg atgcacgagg
    12721 ttactggcat catcggcgtc tcgccctacg ccgagcctga agatgatctc gcgctcttgc
    12781 ccgtcggcca gctcgaatgg aacctggatt gcggcacagg gatctaaagc cgcccctacc
    12841 ttattggaaa gctgcgaccg ggtcatggcg gccggattct tgagcgtgcc gttgcgcccg
    12901 aggaattcag tgcggtcgcc gctgaaggtc cgagtcgcat cgtccacgtc gaagaaggta
    12961 gtccggtcgg cgaactccgt gttgtacgga tttcgcgcga agagcgcacc gctgttgggg
    13021 tcaactttgg tgatgacgtg cagggccgat ttcggccgca gatctcccag tacccattcc
    13081 acatagcccg tggcggagag ccggcgcaat cggccggact cgtttcgcac tttcaacacc
    13141 gcgaacttga tcggcgcgtc cagggccacg taaacccaca gctccgagcg gacgccgcgc
    13201 tccgtgtgct cgaagacgct gtagccgaat ccgtgccggc tgacgtaagg catcgccccg
    13261 cagctgggca gcggcgcggg ggaccagaag tggcccctct cttcgtcacg gatgaaaaag
    13321 gcttctccgc tcgaatcgct tacggggtcg ttgtaccagg gagtgaggcg gaactcgtgg
    13381 gcattctcgc tccaggtgta ggcctggccg ctctccgaga cgagggttcc aaagtgcgga
    13441 ttcgccagca cattcatcca cggtgccggc gtcacttgcc cgtgcgcggt ggtaatgacg
    13501 tactcgcgcc catcaggggt gaatccgccc agcccgttga agaagttcag atcgtggcgc
    13561 ggcaacgcgg cgcccggcga gggttcggcg cggcgggttc gggttggcgt aaggcgcggc
    13621 actgttactt ccaccaggcc gcgacggttg atctgttcgg ccagcgctcc ccgactgtcg
    13681 gtgaggatgg cacgagcaac cgattgaaac agaatgcggt cctcgtccga tatctgctcc
    13741 gcagctcgca cgaagattcc gccgggtcga tccatcacgt gtgcttcgag gcctgcggca
    13801 atcagcccca tgatttgttc gtggagtagt tgccggtaac cggcatgatc ttcgttccag
    13861 atcaccaggt ccaccgccag tcccttcaag cgccagtacg cgtgggcctg cacgagttgg
    13921 cgtaccaact cgatattcgc cgggtcttta atctgcagca gcacgatcgg caaatcgccg
    13981 gaaatggcgt agccccatag accggattgc ccgcggcggt tcttgctgag aacggctgcg
    14041 tcggcgcgca gggaggaatt ggcgtagatg acagagctgg ccaggtggcc ataaagttgc
    14101 gcatcggcat ccgtggcatt gatctgccgc aggaccacct ggctatgtgt ccacgccagt
    14161 tcgaacacgc gatccgcaag atgccggtcc tggtatttcc cgaccaggcc caggcacgcg
    14221 tcccgggttt cgccgatgcc ggagaccata tcgatcgttg ccgattgttc cggatcgagt
    14281 gttattcgat accggatcgc gacaatcgga tccagcaccg agccctggct gcccgagagc
    14341 gccgcggaat cgctcatcgc ttgaggagcg gcgacggtcc gtccgcggcc gatgaaccgc
    14401 atgcgatccg tctcgtatga aacctctccg acgtccgccc cgtgtacggc catcagatga
    14461 aacatccatg gcgcctgctc ctcgagggag cggggccggc gagtgcagag gatcgcctgc
    14521 cgctggcgga tgatctcggt ctgaacaaag agattactga acgccggatg cagtgcgtcc
    14581 gcagcggatg atgcgagaac cacttccacg taactcgtga cgtcgatcgt tctgcttgtg
    14641 cgagagcggt tggtgatgcg gagtcggcgc agttcgatgt catcttctgg cgaaacaaca
    14701 atctcggtat acgtatcgat gtcgtggtcg ctgcgccgga attccgctcg cccctccgag
    14761 aaaatcgctt cgtaagcttc cgatcgcttg agcgccggct gataagcggt tgaccagaac
    14821 accccgctcg tcacgtcgcg gatgtaacag aatgtgcccc agttgtcgca ggtactgtct
    14881 tcgcgccagc gtgtgacggc gaggtctttc cagcgactgt agccgccacc cgcgttcgtg
    14941 accatcacgt ggtatcggcc gttcgataac aactgcacct ccggtatcgg agtatcgggg
    15001 ctgctgagta cgcgcaccgg catctccgga ccgctggaaa tcgcccgaat gtcggaaagt
    15061 tcggcagtgt gcgaatagaa cgccgtagcc tttggaattc gctcctgaag caacaacgtg
    15121 gtcgcctgga acagcggatc cgactcgaat cgcttctgca tcggacggtc caggatcaga
    15181 tcggccagag agagaaagat catgccctgg tgatgtgcca tgaacgaccg aaccacggcg
    15241 ctcgattgcc cgcgtggcag acgtgaaggc gtgtagtcga tcgcctcata aaagccgaat
    15301 tttccttcaa gcccttcagc agcgagtcgc tgcagattca ggcacgcctc ctcgggcgcc
    15361 accatcagcg cgagcgccga tgcatacggc gcaatgacta aatcgtccgc tagtccgcgg
    15421 ttgagcccca ggccgggcac gccaaatgcg cggtactgat agttgagacc agcgttgacc
    15481 gtgttgtagc cggattccga catgccccac ggtacgccgc gctggttccc atacgcgatc
    15541 tgtcgcttca ccgccgcccg ataggtctgg tcgagcagcg tgtgttcata cgtcggcatc
    15601 accagtagcg gcatcaggta ctcgaacatc gaaccgctcc acgacagcag aatcggttcg
    15661 ccaccggcgg tagtgagcag gcgccccagg gcaaaccagc tctcctgcgg cagttgtccc
    15721 tgcgcaatcg ccacgaaact ggataatctc gcttccgaag ccagcagatc gtagtaactc
    15781 gagtcccgcc ggcgctcacc cacgttgtac ccgatagcca gtagatgacg cgtcttgtcg
    15841 aacaggaact catactccat acgcgcgaga tcgcttgatt gcagtgccag gcgctcgata
    15901 gccgcaatcc tttctttggc gcgacggctt gcttccgtga tgagtcgtcg cagctcatca
    15961 agccacccgc tttcctcggg cgtcgcgtcc gagccgagtc gacgcccgat tgccggcagc
    16021 cactccacct caagtctcgc cagttcgcgc agcgttggga tctcaccgat gtccgggaaa
    16081 tcgctgagcc cactcggcgc ggccggcagc aaactccacg gggcgagaaa cgtcagctca
    16141 tcgagggcgc tccggcattg ccgggccagc gcctgtgccc accactttgc ttcgctctcg
    16201 ggagcagcag cgaggccgcc ggccacctct gcagcggacg tcgccagccg ggccagccac
    16261 cgccgcgctg ccgcgaccgt ggcaggccgg gaatcgtatg cagactccag atccttctga
    16321 agttgagcga gctggggaga agcggctcct cccgcagcgt ccacgagtat cctcagcgtg
    16381 tcgctcaagc cctcaaacaa tcgcgacccc aggatcttgt gatcgggaag tgcgagcagc
    16441 cccggccgca atgtcagcag atggcccgcg aggttcccgc tgtccaccga cgaaatgtag
    16501 agaggtggca gcggctgcag agactgcgtg tcgtaccagt tgtagaagtg accttggtgc
    16561 cgttccaagg cttccatcgt gtgaagtgca ttcgccgtgc gctcgatcag ttctccggcc
    16621 ggaatgtagc cgaagtcgta cgcggacaaa tttgcaagca acgcaagtcc catgttggtc
    16681 ggggacgtgc gatgcacgac catggtaacg ggatgctcct gatagttatc cggtggcagc
    16741 caatgatctt ccggaccgac gaaggtttcg aagaacgccc aggtcttgcg ggaaatcttc
    16801 tggagaaaga gggtctggtc ggccgtcaac ctggctgcgc ggcgagcgag cggtcggctg
    16861 atccaccagg cgatggcggg ggcggcaaac cagaggcaca gtatgggccc agccactgcc
    16921 agcgcggccg ggtttgattg cgtcagatag atcgccgcgg cagaggcgat agcgggagcg
    16981 atccacatcg accggcaaga ggcgatgaag tccatgcggc taatgcgatc cagctcgccc
    17041 gacgaattcc attccagaag tcgtttgtgt gtgaccagca tccgcccggc cgtgcgcacg
    17101 atcgcatcca gactgaagaa cgcctcatac ggaaggcata cgagcgtaag tgccgcccga
    17161 gcgaagtgcc ggccagctga gcgtaatgcc gcggccagat gctggcctag cagcacatcg
    17221 tccggtttct ggaacatttc cagaatcgag gcaagcaagg gaggaatcag gatgattccg
    17281 atcaccgaca aggtccagaa ccagaccggt gacaaggccg tccagcccag cagcaacagg
    17341 agcgtcaacg ccaaaggcac gagactgcgc cgaaggttgt cgaaaatctt ccattgggac
    17401 agatccgaga gcggatttct ctggcggctg gcgccggagc ctggaacgtg aggcagcaac
    17461 caccgcgcaa tctgccagtc cccgcgaatc cagcggtgcc ggcggctcac gtccgcgcta
    17521 tagcgagatg gataatcctc gtataaatgc acgtcgctca acagcccggc acgcgcgtaa
    17581 cacccttcca aaaggtcgtg gctaaggatc cggttctcgg gaaagcgtcc cttgagcgcc
    17641 cgctcgaacg catcaacgtc atagatcccc ttgccgatga acgagccttc acggaacagg
    17701 tcctggtaaa catcagagac ggcgcgcgta tacgggtcga tgccgggctc actcccgcac
    17761 agtcgcgcat accgcgaccg gttcgcaccg ggcaggctca cggccatgcg tggctgaagg
    17821 atgccgtacc cctcacagac gcgctgcttg tgttcgtcgt agcgcgcacg attcagcggg
    17881 tgcgccatgg ctcccacaaa ctcccacgcc gaatcgcgcg gtagttgcgt atccgtgtca
    17941 agggtgatca cgtacttcac gttcgataag accgcagtct caccaacgac gagcgagaag
    18001 cgatctgttg agccgccgcg caacagcgaa ttcaattccg caagcttccc tcgcttacgc
    18061 tcgtaaccca tccaaatccg ctcctgagga ttccagcggc gcgggcgatg gaagaggaag
    18121 aaagtgttgc ctttcgcact cctgtacttt tcattcaact cttcgatcct cttccaggcc
    18181 agcagcaaca gcggctcgtc ctccggtagc gtttcctcgt gcgcgtcccg aaaatcagtc
    18241 agcaggccga agtgcaggtg ttcgtcccga ttcgccagga accggacttc aagcgcctcg
    18301 atcagatcct cgatgttttg agcgctcatg agcatggtcg ggaccaccgc cagagtgcgg
    18361 gattccggcg gaattcctcc ggaaaagtcc attcgcggca gcggatgcgg cgtcgccagc
    18421 aacgtcgcca gccaattcac cagcgcaacc gccaaatgac ttgcgcacag cagcgagagg
    18481 atgccaatca gcgcgagcat ccaacccagc aacccgtcgg catgagcttt tgccagtaag
    18541 ctcccggtga agaccaccgt cagcagcaga atcgtgccta gatagaggag caaaggaaac
    18601 cggcagctca ttctctgcaa agcctcgaag gcagaaaggc gcacctccgc cgtttgttcg
    18661 agctgcggca gtcccttgtc gatcaggtag aacccaacat gtgccgctcg atcgtcaccg
    18721 tctttcctgg ccgtgccctc gtgcgccagt tggatcgctt tgcgcgccac ctcgctttcg
    18781 gacaggcggc tgcttctcgc tatcttctcc actacgtgcc ggtagcggtc acgggtggca
    18841 aaatccatct tgccgtagac cccgcccgga tcctcccgca gtttctgctc gacaacgctc
    18901 atggtctcga cgaactcatg ccagtccatc gctcccagaa atcggaggct gccgatgctg
    18961 ttgctcatag agacctggtc ggcggcctgt tgttggtttt ccgaccgcac caactgctcg
    19021 atcgtcaggc catcctcgga gagtcgctgc tcgatccagg tgagcggcaa tgccaaagcg
    19081 gggctctgtc cctgcaaccg gcgcgcaaat tccgcgacaa acgagctcac catcggctgg
    19141 cccgaccgcg ccatatccgc gatcaccagg atcagactct tcgggtcctt ctcggcaacc
    19201 tccatcatct gatccgccca atagtcggcg ccgttacggt cgattcggtt ggcggcgatc
    19261 cgcgctccaa cacgccggag attctcgacc agcgccaggc gtaacatgat agggattgcc
    19321 cacaattcgc ctaacttgag ggctgtgacc ttctggtagg ccgctacgaa gccgctgaga
    19381 ctctccgagt ccactctccc atcgccgtgc gagatcgttt ccagagcaat gtcgtagacg
    19441 cgtggaagcc cggccgatgg gccgttctgc aagcgcggca gttcccggct gtaacccttt
    19501 ggcaaatgtc tcttggccgt gcgaatctgt ccttcgatca gatagaagtt gtcgagcagc
    19561 cattccccgg ccggcgcaat ccggcggttc gccttgaccg ccgccgtcag caggttgcaa
    19621 gccccgatca ggacggcttc attctcagtt agccgtgtca gaagccggtc cggcgcgcgg
    19681 gtcagactca acttgtgcga gcccgccagc gtcctgccat gctgttccat ctggtcggca
    19741 ctgagcagct ccgatcgcag cggcggctcg tcggcggcgt acttttgtgc caagccattt
    19801 ccgcgaaggc gcgccctcaa attgagcaag gtcccgcgaa tagtcttgct gctcaccttc
    19861 aatttttagg tttccttctc tgaagcgaag tttcaacgat ccaggaggtt gagcttaaca
    19921 cttgcacgct agagactccc cggagcaaag acctggcgca gcttaaagaa gcgtttctac
    19981 gtactcgttg attgtgacgc cgctggcagc ctgtcggact tggcggtcgg acaggctgct
    20041 aaaagcaaaa ccgcctgagg atttgggatg aatctaaacc aaatgacccc tgctgtccca
    20101 gcgttggaga cgggagcagg gtcgaactga cgtctctaca gtcctttttt tgccgtattc
    20161 ttgcaggcgg ttttgcatta ggagtttgtc ggaccaaagc ccgacaaact cctggcccga
    20221 tggtcccgtt tgtgaacggg ggtggtagct taggtagtag agccgctgac tggaagcatc
    20281 agcttgcggt tttatcgggg agtaagcggt tcatttcttt tttcaccacc gcgtagcact
    20341 cgcatacgcg tttctcgagc ttcggccgat cgaggacggt gatgtggccg cggcggtagc
    20401 ggatcagccc cgcctcttgc agcttgccgg cggcctcggt gacgccttcg cggcgcaccc
    20461 ccagcatgtt ggcgatcagt tcctgcgtca tggtcagctc gcttgaggcc aagcgatcca
    20521 ggctcaacaa gagccagcgg cacaattgct ggtccaccgc atggtgccgg ttgcagaccg
    20581 cggtctgcgt catctgcgca atcagcgcct gggtgtagcg cagcagcaga tgctgcagcg
    20641 ggccgccgag atcgaattcc gttttcagca cggccgcctt gagccggtag ccgtggccgg
    20701 cgctttgcac cactgcccgg cttggcgtgc tttcaccccc catgaacaac gcgatcccga
    20761 ccacgccttc gttgccgatg accgcgatct ccgtcgaggc cccgttttcc atggcgtaga
    20821 gcaaggacac gatgccgctg gcggggaaat acaggtgccg caacttgctg ccggactcga
    20881 agatcaccag gcccaacggc aacgttacgg gctccaggtg gggcagcagg cgctcgtagt
    20941 ccgccgcagg cagtgcggca agcagctggt tttgctcggg tttatgtgat gcgggcattg
    21001 cgggccggcg ctctccacct tcaggctgcc gttgccgatg cggttgaaag ccgcacttgg
    21061 cggcgaccgg actcgagtgg acagcatact ccaaccgtac gctagcgcac agagcgtcct
    21121 gaacagggag ggaataataa ctgcaggtca acattggcgt tttgctcttt caggaggcag
    21181 ttatgcggac attgataggt gtaattatgg taaccacggc tgcagcggcg actttcggct
    21241 gtgcccaaaa gccgccggca acggtggcgg tgatcgagga caaggtctat acggtgacgc
    21301 cggaccaggt gatggtgaag gccgggatcg tgaccggcga agtcacggag ttgaaggtca
    21361 ccgagcgcgt cgagaagggc tccggccgaa ttgaatcccc ggcaaagctc acggggaagc
    21421 tcaagctgac gaacagctcc acggaccaga ccgtgcgcct cgtcggcggc aggatcctgt
    21481 acatcgatga ccaggggcag ctgatcaaga tcgaggaaac gcggaccgaa ccggccctca
    21541 agttccccac ttcgtacaac gccaccgacc ggctcgatcc aggtcaggat gcaacccagt
    21601 ccgtggaagt ggacttcccc gccgcagcct tgacggcgaa gaagctgaag gaaatccgca
    21661 tcgagctcgt gtacatcccg tcatcgtaca agctgcagac ggccaacttc agcgtgtcga
    21721 ttggcggcca gtcagtcagt cagtaagtca gggacgtggt tccctgccgc cggctccggg
    21781 agccggcggc acaagacgaa caggagcgag agctgtaggg cgatatcccg aacgagggca
    21841 ctatccagaa gaatgcccgg tttcgctcct ccgtgaactc aaataggagt actcgatatg
    21901 accagcaccg aagagcagca gatgcttgcc agcctttccg aaatttcgca ggacatcaag
    21961 gcacttttga cactgatcaa ggatttgaca cggcagcagg gcgacgcaga tgaaaaagcc
    22021 tatggacaac tcgtgaacat agcgcacaag cgctaacggc tgcagggaaa ccggcgggat
    22081 ttaccagttg gcgatctcgc cgacgaactt catcaattct ttttccacga tctccttgtt
    22141 gacctgcgcc agcttgtaat cgccacgcgg gcgggaaccg ctatctgaat tgcgcctcgc
    22201 attgcggccg tgcaatccga tggttcttgt aaatggggcg ccaaagaagg aaagagtggg
    22261 gtaccagtcg tacgggtgga tggatttttt gacacccgcg ggcacgatgt gaatcgagat
    22321 tttcccgccc ggatcttcgg agatgttgat ctcatccccg cgctctttca gcaggtttcc
    22381 gatcgcctcg aacgccggcc tgataaccga tagtttcagc ttcgcaaagt cgccggggaa
    22441 gtcgtccttg ctggttttag cctgcggctt cctgtccgcg cctggagcgc gtgtcctggt
    22501 gaattggtcc aacgcctttt ccagttttga ggtcagttct tcagtcatgg tgttactccg
    22561 tatcggtgct tcggttgaac ttcgtcatcg ctgcatcaag ccaccgcggc gatcggcgaa
    22621 ttcttacgat ttgaatactc gcgtagtgta aggtcgtgca tgtccttgac gatcccgtaa
    22681 caccggcaag aggccgcttc gagacctttc ccatcaagaa tcctgatccc accgcggctg
    22741 tagctgatca gtttccgttg ctgcagggtg ccggccgcct tggtgacgcc gactcgtcgt
    22801 acgccgagca tgtgcgcaag aaattcgtgc gtaagacgga aatcgttcga ctgcatgcga
    22861 tcgcgcgtca tcagcagtga acgggcgagg cgtgcctcca gcagatgaaa gcggctgcac
    22921 acggcggttt gtgtggcttg ggccatcaat tcatgggtgt agtggaacag gtcccgccgc
    22981 aacgacggat ttcgactgaa ttcgcggccg aagcgcgccg attccatccg catggccgtg
    23041 ccggttctct gcaccagggc gcgaatgggc gaaacaccga tccccagcgc caggggaatg
    23101 ccgaccatcc cttcgctgcc gaccagcccg acttccaaca ccgtgcggcc ctccactaca
    23161 gccagcaagg aaaccaggca gtcattgggg aaataggtat gccgaatcgg ctctccgggc
    23221 tgataaagca cttccccgaa agccagctcg accggctcga gaccggcaag caggcgctga
    23281 tactcggcgc gcggcagggc cgcaagcagg ctgttggcaa cagggatggg gatcgcagcc
    23341 ggcatgacgc gaaggttata gcagcgagat tcgactgtat gtgcgctggc gcacagagcg
    23401 tcccggccgc gaaggggaca atagccgcaa cgatggatgg tcctcagaac tggctactcc
    23461 acaacagggc cctccagatt tcgaccccgg cgtgaaattt tcatgccgcg aaacccgctt
    23521 ttgcaaacgt ggcatcggca agagcatcac tcggggtagc acgcccgaaa ggaaataata
    23581 tggaaacgca agccaacaaa tccccccaca tgctgttttg gatcgcgggc atcgcggtga
    23641 ccgtgttcag tgccgtcggt attgcggcca tcatgggttg gatcccgacc tcaaccagca
    23701 gccccagcga gaacattgcc ctcgccaagc agccaacggc gccggcgaag accgccgccg
    23761 cgagagcgcg cgcgaccccg gagcaagccc cggtgcaggt ggcgagcaat aagcccgtcg
    23821 ttagggcggt gtgcgccgaa tgcggcgtga tcgaatcggt gcgcgagatc gacacgaagg
    23881 gcgaaggttc cggcatcggc gcagtcggcg gagccgtcgt gggcggcgtt cttgggcacc
    23941 aagtcggcag cgggcgcggc caggatgttg cgaccgtggt cggtgcggtg ggcggcgcgg
    24001 tggccggcaa cgagatcgag aagcgggtca aatcgggcaa gagctacgag atcaccgtcc
    24061 gctttgagga cgggtcgagc cgcgtgatca acgaagcgaa cgcgccgtcg tggcgggccg
    24121 gcgaccgggt caaggtcatc aacggcgtga tccagtcgaa cgcctgacgc ccgactcact
    24181 taaacgaagt aaaggatact tgccatgcta tataccatcg ctgtcgttct gctcgtcatg
    24241 tggctgctcg gactggtcac ttcctacacc atcggcggtt tcattcacgt acttctggtg
    24301 atcgccatcg tggtcgtcct gctcagaatc attggcgggc gaaaacccgt ttaatccttt
    24361 tcacctttca taacctaaca ggagatacac catgtttcaa agaatcttgg cgacactgat
    24421 attgctgggc atgctgggcg caatcgcggg ttgcaacacg atgcaaggct tcggagagga
    24481 catggagcgg gccggcgaaa agattcagca aaaggcggca cgctaaacgg gaaatgcaat
    24541 gccaggggcg gactccatgg gaatcatccc cacgactgcc gtcccggcaa gcgatgaggc
    24601 gatcgacgcg attgccggca tcgaaatgca gcggcaggta atggagaagg aggctggcct
    24661 tcaagcgtca tgcagctcgg cgctgcgcaa gttgcacgag cttcttgacc gcgagaagga
    24721 agcgctgcag ctgcacggcg cgggcaccgg gcatccggag aacgtcgaag ccgtgatggt
    24781 cgagatcgag cgggtgaagg gattggctgt tatcacgagc cagggcccag cccctaggag
    24841 cgcgtactac cccgggcagc gaaaagcgtc gtggcggaac gcgcaccgga gtcccgccag
    24901 gaacaagggg cgcaggacga tggggcgggc cggcggtcga tgataagtgc tgagtgctga
    24961 gtgaaatgga cctatttttc tctggcgtat ttgaccttcc gtggtggggc cacgtgatcg
    25021 tggccctggt gctgacgcac atcaccatcg tcgcggtcac ggtgttcctg caccgccacc
    25081 aggcgcaccg cgcgctcgac ctgcacccgg ccgtgagtca tttcttccgg ctgtggctgt
    25141 ggctgacgac cggcatggtg acgagggagt gggccgcaat ccaccgcaag caccacgcca
    25201 gggtcgagac cccggaggac ccgcacagcc cgcaggtgct gggaatcaac cgcgtgctgt
    25261 ggggcggcgt gttcctgtac gtcacggaga gccgcaaccg cgaaaccatc gagcgctacg
    25321 gccaggggac ccccgacgac tggctcgagc acaatgttta ctcccggtac gtaaaaacag
    25381 gcctgacgct gatgggcttg gccgacgtca tcctggtcgg catcgtaccc ggcgcactga
    25441 ttttcctggt gcagatcgtc tggatcccgt tctgggcggc cggcgtgatc aacggtatcg
    25501 gccatttccg gggctaccgc aactggccca ccgaggacgc cagcaccaac atcgtgccgt
    25561 ggggcatcct catcggcggc gaggagctgc acaacaatca tcacgccttc acgacttcgg
    25621 cgaagctgtc gagcaagtgg tacgaattcg acattggctg gatgtacatc cgcatcctcg
    25681 aaacgctggg gctcgcgaag gtcaagcgcg tcgcgccggt cccgcgtttc gccgcgccca
    25741 ggctgatccc ggacctgcag acgctgcaag cggtgatcac ccatcgctac gatgtcctgg
    25801 cgaagtatgc caaatcgttg cagcgcatcg gcgccgggga gcagcacgca cacgcactgc
    25861 aaaccatgca tgccatgcgc cgcgagctca tggcgctgtg ggagcggtcc acggcatcca
    25921 aggaacagct ggtgcgccgg ctgcagaact ggtgccagcg cgccgagacg agcggcattc
    25981 aacccttggt ggagttctcg aagcgcttgc gttgttacgc ctaggcatga tcaatcgagg
    26041 agcatcgcgt atgaaaatcc gtcccctgca cgatcgcgtg atcgtcaagc gcttggaaga
    26101 ggaacgcaag accgcctccg gcatcgtgat ccccgatacg gccgccgaga agcccgatca
    26161 gggcgagatc atggcggtcg gcaaaggcaa gattctggag aacggcaagg tccgtccgct
    26221 ggacgtgaag gtgggcgaca agattctgtt cgggaagtac tccggtcaga ccgtcagggt
    26281 taaaggtgat gagctgctcg tgatgcgcga ggaagacatc atgggcgtag tcgaaggcag
    26341 gtaagcagcc tcatattgtt accgattctc aggagaagca catgtcagct aaagacgtaa
    26401 gattccacga tagcgcgcgc aacaagatgg ttgagggcgt caacatcctc gccgatgcgg
    26461 tcaagctcac gctcggtccc aggggccgaa acgtggtgct ggagcgctcg ttcggcgcgc
    26521 cgaccgtcac caaggacggc gtctcggtcg cgaaggaaat cgagctgaag aacaggttcg
    26581 agaacatggg ggcgcagatg gtgaaggaag tggcgagcaa gacttccgac gtagccggcg
    26641 acggcaccac caccgcgacc gtgctagcgc aggcgatcgt gcaggaaggc atgaagtacg
    26701 tggccgccgg catgaatccg atggatctga agcgcggcat cgaccaagcc gtcgtcgcga
    26761 cggtcgatga gttgcgcaag ctttccagac cgtgcaccac caacaaggaa atcgcccagg
    26821 tcggcgcgat ctccgccaac gccgatgaag cgatcggcaa gctcgttgcc gatgcgatgg
    26881 acaaggtcgg caaggaaggg gtgatcacgg tcgaagacgg caagggcctg gacaacgagc
    26941 tcgagttcgt cgaaggcatg cagttcgacc gcggttatct ctcgccttac ttcatcaaca
    27001 aacccgacaa gcagagcgcc gtcctggaag acgcgtatat cctgttccat gacaagaaga
    27061 tctccgccat ccgcgagctg ttgccggtgc tcgagcaagt cgccaaggct ggcaagccat
    27121 tgctgatcgt ggcggaagag gtcgagagtg aagcgctggc gacgctggtg gtgaacggca
    27181 tccgcggcat cctcaagacc tgcgcggtga aagcgccggg cttcggcgat cgccgcaagg
    27241 cgatgctgga agacatggcg atcctcaccg gcggcacggt tatatccgaa gaactcggct
    27301 tgaagctcga gaacgtcacg ttgaaggacc tcggccgctg caagcgcgtt gaagtggaga
    27361 aggagaacac cacgctgatc ggcagcgcgg gcgagaagaa ggccatcgag ggccgcatca
    27421 agagcattcg tgggcagatc gaagatacca gcagcgacta cgaccgcgag aagctgcagg
    27481 agcgcctcgc caagctcgcc ggcggggtgg ccctgatccg tgtcggggcc gcgacggaac
    27541 tcgagatgaa ggagaagaag gcgcgcgtgg aagatgcgct gcacgcgact cgcgcggcgg
    27601 tcgaggaagg tatcgtggcg ggcggcggcg ttgcgttgct gcgttgccgt gaagccgtcg
    27661 ccaggatcaa gggcgacaac cacgaccaga acagcggcat caagatcgtg ctgcgcgccc
    27721 tcgaggagcc attgcgccag atcgtcgcca ataccggtga tgagccctcg gtcgtcctca
    27781 acaaggtcgc cgaaggcaaa ggcaacttcg gctataacgc ggtgaccggc gagtacggag
    27841 atctggtgaa gatgggcgtg ctcgacccga ccaaggtcac gcgctacgcg ttgcagaatg
    27901 ccgcttccgt ggcaggtctt atcctcacca ccgacgccat ggtcgccgac ctgccgaagg
    27961 atgatttgaa cggtggccac ggcatgggtg gaatgggcgg tatgggcggt atggacatgt
    28021 agtcgatacc agggaatcga tatgcaatgg tggggctata gcaaagagca cggctgggtg
    28081 gttctcgacc ggagaattcc gtccaatgcg cccggtctca aggcggatct cctgtttctt
    28141 cgctgccggg atgcaatgat ttttggcgcg aagcgagaga gctggaaccc tcctttgtac
    28201 cggtttgcgc cgaactacat acgtgacctg gcgcctcccg catcggatga ggcggccgcc
    28261 gagctggaag gtttcaaggt tcgatggccc gagttcgaac gtgaactcca gcgcgagtgc
    28321 cgggaaaccg aggagcgagc gcaggccgtc cgcgaccagg aggaaaagat tcggaagcag
    28381 gtcgcgtcag aaaagaagaa acaggccgca gcagccaagg cctgacgcag cgtttggtgt
    28441 cgttaacgag gtgcatttcc gggaacggca tcgaaaacgt cagtctcgag cgtattccac
    28501 cgcaaacggt tccaaagccg aaggcgcctc gtaaccatct gccttgaccc ttgtagcccg
    28561 tgccttgagc cataggaggg acaggacgtg acttcaaggc tgaaggcaga aaatcgggaa
    28621 gtcgccgcac ccctggccct ggaagccgaa gccgcccgca atgtgcgctg gcgtacagac
    28681 ccggcggatt gcctgacgta ccgttgtgca gattggcttt acttactatg tgagggaaaa
    28741 atgaacatcg cgatgtgggt gctggcggga ggcatactcg gctggatagg ctttgcgtat
    28801 ttgaaggtca atgaagaccg gggcatggtg atctcaatca tcatcggcgt attgggtgga
    28861 ttcttcggcg ggaacgtgct ggcgccgatg ctgggcgccg tcgcggacac cccgaatgac
    28921 ttcagcctgt ttgcgatggc tgtcgcgctc gcgagcgcgg cgggatgcct ggccatcggc
    28981 aatctggttt ccaaccgcta cggcgtgtga cttgacgtga gcgagcaggg cggcgggtca
    29041 agatccgcgg caggggtgac cgaactgctg tcggcggtcc ggttccagga agagctgcgg
    29101 cgcgtcgcgc gctttcgttc acgtattcag gtcggggatc ctctggatgc cgcagtgagg
    29161 aagatcacgc agaacccggc gttcacgcaa tcccgtctgc tggcccgcat actggccgcg
    29221 ctgacctacc aagagggcga gttccggcgc gcggaaatcg ccgccttcga cgccgatacg
    29281 ctcgccatgg tcattaccct gatggatgcg cacgctgccg gaacgtccgc gcgcgaggag
    29341 tggatcggcg ccgtcgaggc gacaagaaac gctcttcttg gggtgcagta acgggaagag
    29401 caaggatgaa ggcggaagga tgaaggatga agtaaaaacc gggcccttct gccttttgcc
    29461 ttctgacttc tgctttcagt tgagcgggcg cttcccggtg tattccagca cgtagagccc
    29521 cccgcgcgcc cggtccgcgg cgtagatgat gccgcggtcg tccacgaaca cgtcgctgat
    29581 ccggcacccg cgctgtccct cgggcgtctc cgggaggaag acggcgattt cctccggccg
    29641 gtagggatcg gaaatgtcat agacgcgcaa gccggcgctg aaccacgtgg agactaccgt
    29701 gttctgcagc ctggcggctc ccggctccgt ctcgttctca tggaggttgt gggcgccgat
    29761 gcgaccgcca accttgtgca gctcgccgaa atcgcgtggc gtcggcaggc tcgaaatcat
    29821 cacggggttc gattccagcc gggcatccac gatccagcag cgcttgggcc agtcgaggcc
    29881 gtgatccccg gtcgcttcgt cagtgacgac gaggaggtcg cggtcgaaca gcggcagcac
    29941 cgtgtgcgtg aaaccgggca gcggcggatg gtagtcgagc cggctgatga gtttcggccg
    30001 cgccttgtcg gagataccga ggatcacgat gccggcgtcg atgtagccga tataggcgcg
    30061 gtccgggcgc tcggggtagc agagagtgtg atggggccgg aacgcgaaat cgaaactggg
    30121 cgggacgtgg cgcgtcgggg ccggctccct gtcgcccttg cgctggcccg gcaaccacca
    30181 gcggccggcc tccaccggct gcgacgggtt cgcgaggtcc acgatcatat aaaactggtg
    30241 gtccttggga tcgttcggca cgaaatccgg atgcccggcg gtgatgtgcg cgtagcgccc
    30301 gtccatgaac gagacgtagt ggacgccgtg agcatgcgaa ttgggcgggc ccgacaggtc
    30361 gaaaaaggtg atctcgcggg gctgcgccgg cttcgagacg tcgtagacct ggaaccccgc
    30421 cggcttgagc ccgggcttgt tcacctggta ggcctgaagc aggatgtcac cgcgcagcgc
    30481 gagcgagttg ccgcgcacgt tgttgtgagg cagtggcagt tgccagagca gcttcggctc
    30541 gcgcggctca gtcacgtcga agatggagaa gcacgccggc ccgtgctcgt gggcgatgta
    30601 caggtaacgc cggccgtcct tgagcttcat cgccatgccc tcgccgagat tgggcgcgcc
    30661 gtcggcgtcg tgccgcgcga ggagtttcag gttgtgggcg atgcgtgttc ccgtcatcgg
    30721 cttaccttcc gttcttgtgg aggcggacgg atgctagcac atggtcgcgg gtgacgagtg
    30781 acgggtgtag agtgtcgagt gtcgagtgga aacacttgct tcccgtcata gctttttccc
    30841 tcgacactca tttctcgaca ctcttcccct cgacactcaa tcctcgacac ttttccctca
    30901 tccctcggga acatccagct ccaccagcgg cccgtattgc tggccgccga agacatcggt
    30961 gtcgtccaca tcgccggata tcaccggacg cgggatggtg accttgatcg cggctgccgc
    31021 gtcgtactcg atcaccgata cgacttccgg cgtcaaccgg taggttttcg cgaaccattg
    31081 cgggttgatg accttcgcgt cgcgcacctt ctcgtaagtt gcgcggtcct tgaagatcac
    31141 atcgaaggtc agctcgaacg gccccgagtt cttgctgcgg atcagtttcg cgacctgcca
    31201 gagttttgcc ataggcagcg tttagctttc agctcttagc tgtcagcttg aaccggtttg
    31261 cgttcccgac tgatcgctga gtgcatagct aaatattctc gtagccgatg tggaacatct
    31321 cgaggggcga tgccggctcc accacgtggt tcaggttgaa gcggtagctc gccccgcgcg
    31381 cgatctccgg tggtgagtag gggtaggcga tcgaggtgat gagtccgctc cactccggca
    31441 ccgggtaatg caccgcgata tgcccgaccg atttcatcaa cgccgtcgcc agttcctgcg
    31501 tctgcgcagt cacctggaac agcagcccga cctcgtgccc gatcccggtc tcgggctcga
    31561 gcgggcccat ggcggcgttc ttgccgaaca cgcgcacgtc gaagcggtag cggctctcga
    31621 tgtcctgccc gaacaccgcg cggatccggt cccgcgcggc cttcacgagc ccgtccagcc
    31681 acgagtcgaa ctggcgcagg atgaccgggt cgcgcacgct ccccacgatg aggctctggt
    31741 aaccggcgag ttccgcgccc tcgagcttga tggtgtagcg ctccgccggc ttgaaggtgc
    31801 tgcccgatac ccggacggcg cggtccgaca ccgcctcgta cttcgcgtga acggtgttga
    31861 ggacgcccga gggctcgatg agctcgtacg gcgtggagtt ctcgtaaagg ttgtgcgagg
    31921 cgacgctcac cgggtcgcag cggtagtcgg gattcggcgg ctccaccacg aagctgccgt
    31981 ccgtgaccgt ggcgaacatg cagtcgggat acttgcgcag cacgacgcag gccgcgccgc
    32041 actccaggat cttcgccatg tgccacaccg tcgccgggtt gaagcccctc atcaccggca
    32101 gcgccgcgaa gatggaagtg tcgctcgagc ggccggcgat gatgacgtcc gcgccgcgct
    32161 cgagcgcttc gacgtagggc tcgatcccgc acatgccgac gatgtgcgcg ctgcggtcga
    32221 tcaccgcctc gttgatctcg ggcgcgttgg caagcggcgt gattttccgc tcccgcagcc
    32281 tgcgcttgag gtaacttttg tcctgctcgc tcgcgatcgt ggcgagcttg aaggagagct
    32341 tctcctcgcg cgcgatctcg cgtgcgatgc ccgccaggtg ctcgagctgg ccgtcggtgc
    32401 cggcagtgca ggcggagccg acgatggccg ggatcttcgc cgcgcgcgcg gcgagcagga
    32461 tcaggcgcag atcgcgcttg taagccgaat cggagaacag cgtctcgcct gaaccgaggt
    32521 agtaggggcc cgggtcggtc gagcctgagt cggcgccgat gaaatccggc ttgagcgcca
    32581 tggcgcgctt gagcgtctcc tcgcggaagc cggagccgag gatgccggtg gcgga
//
LOCUS       DBNGWZ010000617        17392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3500521, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000617 DBNGWZ010000000
VERSION     DBNGWZ010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17392
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3500521"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16CX_19195"
     CDS             <1..730
                     /locus_tag="AC16CX_19195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3014668.1"
                     /translation="EHDGFRMLLAQRNRAAFTIQNRITLTLCRRLRDLNAKILACDTP
                     EDVAPPLAARAGDGMRRGRCLFDFKAFLPVLPVFRRFSAAEVASFAGMTEVIEAERGS
                     VLFRQGDAAEVCYMVVRGAVEITGSRDGRRHRIGILGPGRLCGTLAMIEGQAHSMGAA
                     ARENAVLLEIGAPSFNRLFNENDRISAHFQDAINRELLQALARTNNHLTRLISQARIR
                     GGRQEKKQVEELGRALGEQDCRAA"
     gene            complement(839..1762)
                     /locus_tag="AC16CX_19200"
     CDS             complement(839..1762)
                     /locus_tag="AC16CX_19200"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR3014669.1"
                     /translation="MCRALAYLGQPVLLDNLLYQPDSALVKQSFMPKMLGLLNLAGFG
                     FKAWDRGSFDPDKPFSYASTELPVYDRNLKALAEKIRSTCVLAHVRGVPYSTEGVISR
                     QNVHPFQFPGCRITLAHNGNLDRMRDMKPLLAEHVRPEFVKMITGTTDSEWIYALLVS
                     NLEDPARYATADEIVRAVEKTIAVIRETRARLGIALSSPVNLFITTGNQLAAVRYCFD
                     FGCYRTDDPARVIEANFTYYSLWYTSGSEYGYHDGEWKMIGGADTADSIMIASEPLTR
                     DTSTWLEVPEYSMIYADTRSGRPTVEIHYLD"
     gene            complement(1886..3103)
                     /locus_tag="AC16CX_19205"
     CDS             complement(1886..3103)
                     /locus_tag="AC16CX_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3014670.1"
                     /translation="MALPLEGVKVLDFTAIMAGPYCTLMLADMGADVVKIESIPEGDG
                     SRRFDPQVNGESYCFAVLNRNKKSVALNMKDARGRDIFMKLARDADIVIENYRPGVTR
                     KLGIDYDTIKAINPDVIYASISGFGQTGPYSSKGGFDIIAQGMSGIMTMTGEPGGRPA
                     KVGIAMNDIAAGTTALYAILGAYIKRLKSGEGIYLETSLLEAGMAWTFWEFGAYYGGG
                     EIPIATGTRHRRSTPYQAYRTRDAYVTVGANNEKLWTAFCSQVLERRELLKDPRFRML
                     AARLKNIDALQEEIEKTLATRTTDHWVKKLDAAGVPGGPVFNYVQAFENEHSGARNMV
                     AEIDHPKIGRMKIIGNPVKTNAELARSRTPAPWLGQHSAEVLKSLGYSDEQVAALFAE
                     SVVYDRLREKATA"
     gene            3292..4257
                     /locus_tag="AC16CX_19210"
     CDS             3292..4257
                     /locus_tag="AC16CX_19210"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014671.1"
                     /translation="MKLVFATLAAVVSLIPAAGVLAQAYPVKPVRVLIPWPPGGANDI
                     VGRIVFQKMSENLGQQFVIENRGGASGTIGADLVAKGPADGYTVMVHSATHVANPHLY
                     KKLPYDTLKDFTGVTALAVQVGMLVVHPSLPAKSVKEFIALGKARPGQIVYGSSGNGS
                     FVHLAMALLNAMTDTKMIHVPFKGGGPSVIALSSGEIQAMTATIGSVFHHVQAQRLRP
                     LGITSERRVSQFPDVPTIAEGGVPGYEFTAWIGAFVAAGTPRPIVDKLNAEIRKALDH
                     PEASKILSSQTLDPLPMSPEQFAQRLKSDYDKYEKVIQISGAKID"
     gene            4286..5251
                     /locus_tag="AC16CX_19215"
     CDS             4286..5251
                     /locus_tag="AC16CX_19215"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014672.1"
                     /translation="MAPFFTILVAVALLMPAAGAFAQAYPVKPVRVLIPWPPGGSNDV
                     VGRIVMQKLSEALGQQFVIDNRSGAAGTIGADVVAKAPPDGYTIMVNSTTHLGNAHLY
                     KKLPYNTLKDFTGVGMMVSQPGALTVHPSLPVKSVKELIALARARPGEILYSSSGSGS
                     APHLSMALFISMTGIKMVHIPYKGGAPQVTALVAGEAQVSLATVATVIHHIRAGKLRA
                     LGVNSSNRVAVLRDTPTIAEAGVPGYEMSPWIGVFAPAGTPRPIIDRLHAEITRVLRM
                     PDIAQSLSNQSLEPLFESTNEFNTRIRADYEKYGKLIQLAGAKLE"
     gene            complement(5281..6744)
                     /locus_tag="AC16CX_19220"
     CDS             complement(5281..6744)
                     /locus_tag="AC16CX_19220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014673.1"
                     /translation="MKKALKKIQTDVLVVGGGTAGFVAAIAAARNGAKVALVEQRDHL
                     GGTHSGGMVMMIRSMRHMRAPKGAEHKKVMITSYESSFDDEQLVFSIAQEYLDRMLEL
                     GSAWGSKGQATARQLFDPEIAKWVIEQMVVEAGVDVWYYSQTTDLLQSDSSVNGAIIE
                     NLQERIEISAQVTIDTTGDGNVCAAAGARFEMGDPEDGHCQPMTLYFIVGGVDLDRTL
                     AYIKDHPDEFNQQYVDTILRLRHEGKPITVLPFKARIREALEKDEYPIPYGLEKVNPD
                     TLTYMARPMFRNGKFRYDLMSHNMDMAYNVDATDRRALTRATIGMRDVAVRMTAFYRK
                     YVPGYENSYLVHTAQSVGVRDSRRIIGDYTLTKEDVLSGRRFEDGIGRYGSVVDVHDK
                     DGKQSLMLAEVGGSGWFHIPYRVLLPRGIRNLLVAGRCISADYVAQGCTRSQAACMMT
                     GQATGTAAALAVKAGVEPRAVDIAALQRVLVSQNQII"
     gene            complement(6747..7748)
                     /locus_tag="AC16CX_19225"
     CDS             complement(6747..7748)
                     /locus_tag="AC16CX_19225"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014674.1"
                     /translation="MKPHDSPLSREAAGSGKRFAMAAVAFLFAMPAWAQQWPARSVRV
                     VHGFASGAAIDVMSRPLAAKLSELLGQQFVVDSRPGATGVIANELVARAAPDGYTVLA
                     APGSSLASTPHIRKVPFDPLKDFAPVGLTTTFSFVMVAHPAVPARTAGDLVTLGRRRT
                     DLTFGSTGVGSAFHLAGELFSQMARIPLLHVPYRGGGTGAITDLIGGRVDFMWESPAV
                     VAPYVRAGRLKALGVTGPTRNPLLSEVPTIAESGLKGYSLTGWHGMLLPAATPRAILE
                     RLNTALNQALQSPEVKALWAKMGMDTPASTPEQFAAFLRSEFDRYGKLVASLGLKAN"
     gene            complement(7773..8765)
                     /locus_tag="AC16CX_19230"
     CDS             complement(7773..8765)
                     /locus_tag="AC16CX_19230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014675.1"
                     /translation="MKQSSLVGISFMTAAMGLCFAPPLSHAQTYPSKPIRLIVPNVPG
                     GGTDTVARLIAEKVSAALGQQIIVDNRGGAGGRIAAEFVARSPKDGYTLLLGSAATLI
                     TGPALDADRRYDPIKDFAPVSLAGTTAYMLVTHPSLPARTVRELINLAKASPGRIAYA
                     TTGQGGPAHLGTELFQAMAQVKMIHVPYKGGAPAMMSLLQGETFVMISNFLTALPPVR
                     ANRVRALGVTSLKRTSLAPEIPTIDESGMRGFELQQFYSVVAPAGTPGEVLQRLNQEI
                     VQVLPIPDVKQRLAKEGIELRTSTPAELAQLNAEQFAKWTEVIRKAGIKRSRQD"
     gene            9186..9332
                     /locus_tag="AC16CX_19235"
     CDS             9186..9332
                     /locus_tag="AC16CX_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014676.1"
                     /translation="MNLGISGKTALVVGASEGVGYESTKGLREEGARVLICSRIAGKL
                     KYNN"
     gene            9345..10202
                     /locus_tag="AC16CX_19240"
     CDS             9345..10202
                     /locus_tag="AC16CX_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014677.1"
                     /translation="MALKYPLVHVDDVRPENMKEGEGWAISEFRLPISGRNGSSTTLF
                     HSIFRPGSTHARHLHRNCDEIAVYVKGHGAVGQRNERTEVRAGHCRLMPKGAEHFFFN
                     ETKNEETLVIGFYVGAKSVADTGYEFLGPVTEADLRMPRRENFSDGVLVHVDDVKPSQ
                     MNVKDGWSITDFRIPIGRHNGSPTALFWAKFMPGAVHKKHRHENCEELYYVIRGHGLA
                     GAGPDRAEVRGGHVHYIPKGVEHFLYNLSATEPLEAIGIYVGAGSVEETGYAYTGEVT
                     EADIRARTA"
     gene            complement(10244..10375)
                     /locus_tag="AC16CX_19245"
     CDS             complement(10244..10375)
                     /locus_tag="AC16CX_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014678.1"
                     /translation="MVADSFSALVHGSHGTYTAKGAAGVTVFSSLNEMVEGIRARLR"
     gene            complement(10369..11508)
                     /locus_tag="AC16CX_19250"
     CDS             complement(10369..11508)
                     /locus_tag="AC16CX_19250"
                     /inference="COORDINATES: protein motif:HMM:NF012678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3014679.1"
                     /translation="MYYGADSLQQLPAELDRLACRRTVVFCGQTLAHRTEGLRTVTEA
                     LGDRYAGVFDGVKAHSPLPAVLAGVEVLRALKADAIIAVGGGSAVVTARASSILLAEA
                     RDIHELCTQYRNGKPPLSPKLQRPKLPQFVVATTPTTAYGKAGSAVVDPASGQRLALF
                     DPKTRTSALFIHPELALTAPPQLALGAGVQAFSMAVQGIESRNRDPLADALLVHALRL
                     LGRYLPGLLADPDDAETRGQLMVGALLSGLGTDHAPSGLTSAVAHCIGTRFDLNNGIT
                     NALMLPHAMRFNAPATGNRLSLVAEALGAGVPDNADAAVAGAVRATEELFARLGMPKR
                     LRDVGIPEDALPQLAQDAAVDWFLHQNPRHVTDVTELLEILKAAW"
     gene            complement(11575..12987)
                     /locus_tag="AC16CX_19255"
     CDS             complement(11575..12987)
                     /locus_tag="AC16CX_19255"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014680.1"
                     /translation="MGSHEIAVDASEQTLDAFIDLSQALRWEQVPQATRASVRRELLD
                     YLGAAIAGRAVAGLPRWLEVLIDMGGRPDARVIGGPRVPAPTAALCNGYFGHVLEFDD
                     THDEAVLHAGSAAIPAAFAASGLRGEVSGREFCEAVLLGIELTCRLGVATKLNLVEGG
                     WIYTALFGHFGATLAAARLVDQRAEVLRNALGIVYCLASGNHQSTREGAPTKHVQPGF
                     AAANAITAALMASGGLPGVKHPLTGEDGLSRVYLHDLFDPARATRGLGTEWEFDRLSI
                     KPYPTCRLTHPAISAALDLRAQLGRDTEKIERVELVIGPQAHDVVGRDVSEKRTPQTR
                     VNAQFSVYWSVASALAYGEVTPRQLAEEIPPSPKLREWIGKISGTPDPTAAQRDVGGC
                     KLRAHGAFGTREVEQTSAKGHPDNPLSDDELLAKFKANLRYAKVGDGQAAELADAIME
                     IDSLSDVRPLADALSGAVSS"
     gene            complement(13008..13994)
                     /locus_tag="AC16CX_19260"
     CDS             complement(13008..13994)
                     /locus_tag="AC16CX_19260"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014681.1"
                     /translation="MKAPIQRLPLCALALFCVSVAGTGAAQTPDAIRFPTKPIRMISP
                     FPPGGSNDILARLVAHKMSERLGQQTIVDNRPGASGAIGTDLAVRAPADGHTLVMVAT
                     TYAQIPAIQKLPFDPVKSLAPIAQVATGPNAICSHPNFAAQTVKQLIALARAKPDELR
                     YASTGTGGFNHFSAELFNQLAKVKLIHIPYKGGGPAMLDVMTGQVEVVFGSLIQALPH
                     IRSGKLKPLGVGSPKRSALLPQVPAIDETVPGYDGSIWWGVLAPAGVPGPIVNKLNGE
                     INAILREPEMAKRLSAEAAEPVITTPEAFGKLIVNDLAKWGRIAKQAGIRAE"
     gene            complement(14030..15025)
                     /locus_tag="AC16CX_19265"
     CDS             complement(14030..15025)
                     /locus_tag="AC16CX_19265"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014682.1"
                     /translation="MKMSQFAVALAAGAAVLALGTHADHAPAQPVDVSKYPSRPIRLI
                     IPFPPGGSNDILGRFIAQKMTERLGKTTIADNRPGADGIIGTELASRSPADGHTLLIV
                     STSYTMNPAVHKLPYDPLKSLVPIAQIASGGNVIATHPNFPARTAKDLVALAKAKPGQ
                     IRYASSGIGGFNHFGGEQFNMLAGVRLVHIPYKGGGPSMLDVMTGQVEVVFGTLIQAI
                     PHIRSGKLKALGVGSAKRSPLLPDVPTVSESGVPGYDGSVWWGVLGPAGIPGAIVTLL
                     NTEIGTIVSEPEMAKRLVAEAAEPVVSTPEAFGKLIVNDIAKYLRIAKQAGIRAE"
     gene            15136..16404
                     /locus_tag="AC16CX_19270"
     CDS             15136..16404
                     /locus_tag="AC16CX_19270"
                     /inference="COORDINATES: protein motif:HMM:NF016138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HIR3014683.1"
                     /translation="MPIRSGPSSTLDFSRHVRADDVVTWPQGTGEPLGLTRRLVEQRH
                     ALPPLELFVGMMTSKTLKPEHADRFRFRGLNGAGTTRALTTANLLDIIPAYVSAVPAL
                     IRSRAIRVDVVLLRARPHPKPGFFTVGVMADFIPAMVQAARCVIAEIDERLPVTSQDA
                     LVAEADIDVLTECDADEILLPDPEPDELDKRVAAHVAALIPDGATIQVGIGGLPVAVC
                     RALENHRDLGVHSGVISDVVVDLVEKGIVTNARKGIDAGRIVTGGLFGSRRLMDFADG
                     NDVLEMRSSEYTHSQQVLARVQSLYAINSGVEVDLTGNVNSEVAAGRYLGAIGGQPDF
                     VRGAFASPGGRAIIALPSTTPDGKHSRIVASLEGRPVTTTRADVDIVVTEYGVADLRG
                     RSFSERAERLRAIAHPDFRDDLVKSISKAA"
     gene            16405..17217
                     /locus_tag="AC16CX_19275"
     CDS             16405..17217
                     /locus_tag="AC16CX_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3014684.1"
                     /translation="MQPGTKTNRADSGIVLERSGEGIVQIRLNRPERLNALGMDMVEA
                     FQAAIADAIAGRARVVLVRGTGRAFCAGADLKERRTMDEAARVRHNRAINAAVDALAA
                     APMPTIAVINGLAMGGGCEIALACDLRYAAEDAQIGLTEARIGAIPGAGGTQRLPRVI
                     GASRALELILTGEPVTGKRAEEIGLVNGSVAADKLDAHVMRIATVLASRSPTGAQTTK
                     RLVYRGLEMTLADGLVQEGLALREILASPDYAEGLAAFAEKRQPRFGGGTIT"
     gene            17214..>17392
                     /locus_tag="AC16CX_19280"
     CDS             17214..>17392
                     /locus_tag="AC16CX_19280"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3014685.1"
                     /translation="MSRAASTHRSKAEHVAQQLLERIIAAKLKPGSSFATEAELLSQF
                     NVSRPTLRESLKLLES"
BASE COUNT         3003 a         5573 c         5673 g         3143 t
ORIGIN      
        1 cgagcacgac ggtttccgca tgctgctcgc ccagcgcaac cgcgcggcgt tcaccataca
       61 gaaccgcatc acgctcacgt tgtgccggcg gctgcgcgac ctcaacgcga agatactggc
      121 ctgcgacaca cccgaggacg ttgcgccgcc gctcgccgcg cgtgccgggg acggaatgag
      181 gcgcgggcgg tgcttgtttg acttcaaggc attcctgccg gtcctgccgg tgttccgtcg
      241 tttcagcgcc gcagaggtcg cctcctttgc gggcatgacg gaagtcatcg aggcggagcg
      301 cggtagcgtc ctgttccggc agggtgacgc cgccgaggta tgctacatgg tcgtgcgggg
      361 cgcggtcgag atcacgggct cgcgggacgg gcggcgccac cgcatcggca tcttggggcc
      421 gggccggctg tgcggcacgc tcgcgatgat cgagggccaa gcgcacagca tgggcgcggc
      481 ggcgcgcgag aacgcggtgt tgctggagat cggcgcaccg tctttcaacc gcctgtttaa
      541 cgaaaacgac cggatttcgg cgcacttcca ggacgcgatc aaccgcgagc tcctgcaggc
      601 gctcgcgcga accaacaatc acttgacgcg cctcatcagc caggcgcgca tccgcggcgg
      661 gcggcaggag aagaagcaag tggaggagtt ggggcgggct ttgggggagc aggactgccg
      721 cgccgcatga cggcgggaag cgtgataggt gataagtgat aggtgatggg tgtcacggca
      781 ggccaaaccg ccgattctta tcacttatca ctcgtcactt atcacgcagt tgtcgacgct
      841 agtcgagata gtggatctcg acggtcggcc gaccgctccg ggtgtcggcg tagatcatcg
      901 agtactccgg cacctcgagc caggtcgagg tgtcgcgcgt caacggctcg gaggcgatca
      961 tgatcgaatc cgcggtgtcc gcgccgccga tcatcttcca ttctccgtcg tggtagccgt
     1021 actcgctgcc tgaggtgtac cagaggctgt aataagtgaa gttcgcttcg attacccgcg
     1081 ccggatcgtc ggtgcggtag cagccgaagt cgaaacagta gcgcaccgcg gcgagctggt
     1141 tgccggtcgt gatgaagaga ttgacggggg agctcagtgc gatgccgagc cgtgcccgcg
     1201 tctcgcggat gacggcgatg gtcttttcca ccgcccgcac gatctcgtcc gcggtcgcat
     1261 agcgcgccgg gtcttccagg ttggagacga gcagcgcgta gatccactcg ctgtcagtgg
     1321 tgccggtgat catcttcacg aattccgggc gcacgtgctc cgcgagcagc ggcttcatgt
     1381 cgcgcatgcg gtcgagattg ccgttgtgcg cgagcgtgat gcggcagccc gggaactgga
     1441 agggatggac gttctgccgc gagatcacgc cctcggtgct gtagggcacg ccgcgcacgt
     1501 gcgcgaggac gcaggtcgag cggatcttct cggcgagcgc cttgagattg cggtcataca
     1561 ctggcagctc ggtggaggcg tagctgaacg gcttgtccgg gtcgaacgag ccgcggtccc
     1621 aggccttgaa gccgaagccc gccaggttca gcaatcccag catcttcggc atgaacgact
     1681 gcttcaccag cgcggaatcc ggctggtaga gcaggttatc gagcagcacc ggctggccga
     1741 ggtaggcgag agcgcgacac atgcgtcaag tcgaaagtcg aaagtcgaaa gtcgaaagtg
     1801 caaagcagca gaatgcggct ttgaagcgtc ttgaaaccgt tctcagcttc actttcgcct
     1861 ttcgccttcc gacttccgcc ttaggtcagg cggtggcctt ctcgcggagg cggtcgtaga
     1921 ccacgctttc ggcgaagagc gcagcaacct gctcgtcgct gtagccgaga ctcttgagga
     1981 cctcggcgct gtgctgccct agccacggcg cgggtgtgcg ggagcgcgca agctccgcgt
     2041 tggtcttcac ggggttgccg atgatcttca tgcgcccgat cttcggatgg tcgatttcgg
     2101 ccaccatgtt gcgcgccccg gaatgctcgt tttcgaacgc ctgcacgtag ttgaagaccg
     2161 gcccccccgg gacgcccgcc gcatcgagct tcttcaccca gtggtcggtc gtccgagtag
     2221 cgagcgtctt ctcgatttct tcctgaagcg cgtcgatgtt cttcaagcgc gcggcgagca
     2281 tcctgaatcg cgggtccttc agcagctccc ggcgctccag cacctggctg caaaacgccg
     2341 tccacagctt ttcgttgttg gcgcctaccg tgacgtaggc gtcccgggtg cggtacgcct
     2401 ggtagggcgt ggagcggcga tggcgcgtcc cggtcgcgat ggggatttcg ccgccgccat
     2461 agtaagcgcc gaactcccag aacgtccacg ccattcccgc ctcgagcagg gacgtttcga
     2521 ggtagatgcc ttctccggac ttcaggcgct tgatatacgc gccgaggatc gcgtacagcg
     2581 ccgtcgtgcc ggcggcgatg tcgttcatcg cgatcccgac cttcgcgggg cgtccgcccg
     2641 gctcgccggt catggtcatg atgccgctca tgccttgcgc gatgatgtcg aacccgcctt
     2701 tactgctgta cggcccggtc tggccgaagc cggaaatcga ggcgtagatg acatcggggt
     2761 tgatcgcctt gatcgtgtcg tagtcgatcc cgagcttgcg ggtgacgcca gggcgataat
     2821 tctcgatgac gatatcggcg tcccgcgcga gcttcatgaa gatgtccctg ccgcgcgcgt
     2881 ccttcatgtt gagcgcgacg ctcttcttgt tgcggttcag gaccgcgaag cagtagctct
     2941 cgccgttcac ctggggatcg aagcgccgcg agccgtcgcc ctcgggaatg ctctcgatct
     3001 tgacgacatc ggctcccatg tcggcgagca tcagcgtgca gtatggccca gccatgatcg
     3061 cggtgaaatc cagcaccttt acgccttcca ggggaagcgc catgcgaaat tctcctgacg
     3121 accagcgaac cgtgcggatt ccggctcgac atcggtgtcc aatatatgat ggaattgtag
     3181 cgcgccgact ttgcccgcgt cagcaaggtt ttcagggcag gtcgcgcgag gcggctctca
     3241 gcgccgcatc tcggtcccac ccgacagaac gcacagaaga ggagggagtt catgaagctc
     3301 gttttcgcga cccttgccgc cgtcgtgtcg ctaatccccg cggcaggcgt cttggcgcag
     3361 gcatatccgg tgaagccagt gcgcgtgctg attccgtggc cgccgggagg cgccaacgac
     3421 atcgtcgggc gcatcgtgtt ccagaagatg tcggagaacc tcggccagca gttcgtgatc
     3481 gaaaaccgcg gcggggcatc cggcaccatc ggcgccgatc tcgtcgcgaa aggccccgcg
     3541 gacggctata cggtgatggt gcattccgcc acccacgtcg ccaatccgca cctctacaag
     3601 aagctgccgt acgacacgct gaaggacttc accggcgtga ccgcgctcgc ggtccaggtg
     3661 ggcatgctgg tcgtccatcc ctcgctgccg gcgaaatccg tcaaggagtt cattgccctc
     3721 ggcaaggcgc gtccgggcca gatcgtctac gggtcctcgg gcaacggcag tttcgtccac
     3781 ctggcgatgg ccttgctcaa tgccatgacc gatacaaaaa tgatccacgt tccgttcaag
     3841 ggaggcggcc cgtcggtgat cgcgctctcc tccggcgaaa tccaggcgat gaccgccacc
     3901 atcggatcgg tgttccatca cgtgcaggca caacggctgc gtcccctggg catcacctcg
     3961 gagcggcggg tgagccagtt cccggacgtg ccgacgatcg cggaaggcgg cgttcccggc
     4021 tacgagttca cggcctggat cggcgccttt gtcgcggccg ggacgccccg gccgatcgtc
     4081 gacaagctca acgcggagat ccggaaggcg ctcgaccatc ccgaggcctc gaagattctc
     4141 agctcccaga cgctcgaccc gctgcccatg tcgccggagc agttcgccca gcgcctgaag
     4201 tcggactacg ataaatacga aaaggtgatc cagatttcgg gcgccaagat cgactgaggc
     4261 gcccaccatt ttcgaggaag agatcatggc accgtttttc acgatccttg ttgccgttgc
     4321 attgctgatg cctgcggcag gcgcctttgc ccaggcgtac ccggtcaaac ccgtgcgcgt
     4381 gctgatcccc tggccgccgg gcggctccaa cgatgtggtg ggccggatcg tcatgcagaa
     4441 gctgtcggag gcgctcggcc agcagttcgt gatcgacaac cgttcagggg ctgccggcac
     4501 tatcggcgcc gatgtcgtgg caaaagcgcc gcccgacggc tataccatca tggtgaattc
     4561 gaccacccac ctcggcaacg cgcacctgta caagaagctg ccgtataaca cgctcaagga
     4621 cttcaccggc gtcggcatga tggtgtctca acccggagcc ctgacggtcc atccttcgct
     4681 gccggtaaag tcggtcaagg agctgatcgc gctcgccagg gcgcgccccg gcgagatcct
     4741 gtattcgtcg tcgggcagcg gcagcgcgcc ccacctctcc atggcgctgt tcatctcgat
     4801 gacgggaatc aagatggttc acattcccta caagggtggc gcgccccagg tgacggctct
     4861 ggtggcgggt gaggcgcagg tttcgctggc gacggtcgct actgtgatcc accacatccg
     4921 tgcaggcaag ctccgtgccc taggcgtgaa ttcatcgaac cgcgttgcag tcctgcgcga
     4981 tactccgacg atcgcggagg ccggcgtccc cggctacgag atgagtccgt ggatcggcgt
     5041 gttcgcgccg gccgggacgc ccaggccgat catcgaccgg ttgcacgccg agatcaccag
     5101 ggttctgcga atgccggaca tcgcacagag cctctcgaac caatcgctcg agccgctctt
     5161 tgaatcgacc aacgagttca acacgcgcat ccgggcggac tacgagaaat acggcaagct
     5221 gatccagctc gctggcgcca aacttgagtg aaatttgtcg gagccaagtc cgacaaacta
     5281 ctagatgatc tggttctgcg agactagcac cctctggagc gcggcgatat cgaccgccct
     5341 cggttctact cccgccttga cggccagggc agccgcggtg ccggtcgcct gccccgtcat
     5401 catgcaggcc gcctgcgaac gggtgcagcc ctgggcgacg taatccgccg agatgcaccg
     5461 tcccgcgacg agaaggttcc ggatgccgcg cggcagcagg acccggtacg ggatgtggaa
     5521 ccaacccgac ccacctactt cggcgagcat gagggattgc ttgccgtcct tgtcgtggac
     5581 atccacgacg ctgccgtatc gcccgatacc gtcctcgaag cgcctgccgg agagcacgtc
     5641 ttccttcgtg agcgtgtagt cgccgatgat gcgccgggag tccctcacgc ccaccgactg
     5701 cgcggtatgg accaggtacg aattttcgta gccgggcacg tatttccggt aaaacgcggt
     5761 catcctgacc gcgacgtccc tcatgccgat cgtcgccctg gtaagcgccc gcctgtcggt
     5821 cgcatcgacg ttgtaggcca tgtccatgtt gtgtgacatg aggtcgtaac ggaacttgcc
     5881 gttcctgaac atgggcctcg ccatataggt gagggtatcc ggattcacct tttcgaggcc
     5941 gtacggtatg gggtactcgt ccttctccaa cgcctccctg atcctggcct tgaatggcag
     6001 cacggtgatc ggcttgcctt cgtgccggag gcgcaggatg gtatcgacgt attgctggtt
     6061 gaattcgtcc gggtggtcct tgatgtaggc gagcgtccgg tctaggtcca cgccgcccac
     6121 gatgaagtag agcgtcatcg gctggcagtg gccgtcttcc ggatcgccca tttcgaagcg
     6181 cgcgccggcg gcggcgcaca catttccgtc ccccgtcgtg tcgatggtga cctgggcgct
     6241 gatctcgatc ctctcctgaa ggttctcgat gatcgcgccg ttgaccgagc tgtccgactg
     6301 cagcaggtcg gtcgtctggg agtagtacca gacgtccacg cccgcctcca cgaccatctg
     6361 ctcgatgacc cacttcgcga tttccggatc gaacagctgc ctcgccgttg cctggccttt
     6421 cgatccccat gccgagccca gttccagcat ccggtcgaga tactcctggg cgatggagaa
     6481 caccagttgt tcgtcgtcga acgagctttc atagctggtg atcatgacct tcttgtgctc
     6541 cgctcccttc ggggcgcgca tgtgccgcat cgagcggatc atcatgacca tgccgccgga
     6601 gtgagtgccc cccaggtgat cgcgttgctc gacgagcgcg accttcgcgc cgtttctcgc
     6661 cgccgcgatc gcggccacga agccggcggt cccgccgccg accaccagaa cgtccgtctg
     6721 aatcttcttg agcgctttct tcactttcag ttcgccttca gaccaagcga agcgacgagt
     6781 ttaccgtagc ggtcgaactc ggagcgcaga aacgcggcga actgctcggg cgtcgatgcc
     6841 ggcgtatcca tgcccatttt cgcccagagt gctttgactt cgggcgattg cagcgcctgg
     6901 ttgagagccg tattgagccg ctcgaggatc gcccgcggtg tggccgccgg caacagcatc
     6961 ccgtgccagc ccgtcagcga atagcctttc aaaccgcttt cggcgatcgt cggcacttcg
     7021 gacagcaatg gattgcgcgt cggaccagtg acgccgagcg ctttgagccg gccggcacgc
     7081 acgtaagggg ccaccaccgc cgggctttcc cacatgaaat caacgcgtcc accgatcagg
     7141 tcggtgatcg cgcccgtacc gccgccgcgg tacggcacgt gcaacaacgg aatgcgggcc
     7201 atctgcgaga acagttcccc ggcgagatga aatgcgctgc cgacgcccgt ggagccgaag
     7261 gtgagatcgg ttcgcctgcg gccgagcgtg accaggtcgc cggccgttct cgccggcacc
     7321 gccgggtgcg cgaccatcac gaacgagaac gtggtggtga ggcctaccgg cgcgaaatcc
     7381 ttgagcggat cgaagggcac cttgcggatg tgcggcgtgc tcgcaagcga cgatccgggc
     7441 gcggcgagca cggtgtaacc gtcgggcgcg gccctcgcca caagctcgtt cgcgatcacg
     7501 ccggtggcgc cgggccggct gtccacgacg aattgctggc cgagcagctc cgagagtttc
     7561 gcggccagcg gacggctcat cacgtcgatc gccgcgccgg acgcgaatcc gtgcacaacc
     7621 cgcacgctgc gcgccggcca ctgctgcgcc caggccggca tcgcgaaaag gaacgcgact
     7681 gcggccatcg cgaagcgttt gcctgacccc gccgcttcgc ggctcagcgg tgaatcatgc
     7741 ggtttcatga caattctcct cccccgatca actcaatctt gcctggagcg tttgatgccc
     7801 gccttgcgga tcacctctgt ccatttcgca aactgttccg cgttcagttg agcaagctcg
     7861 gccggcgtag atgtccgcag ttcaatacct tcctttgcaa gccgctgttt cacgtctggg
     7921 atcggcagca cctgcacgat ctcctgattc agcctctgca gcacttcccc gggtgtgccc
     7981 gcgggggcga cgacagagta gaactgctga agctcgaagc cgcgcatgcc cgattcgtcg
     8041 atcgtcggta tctcgggggc gagcgaggtg cgcttgaggc tggtgacccc gagcgccctc
     8101 acgcggttcg cgcgcaccgg cggcagcgcg gtcagaaagt tgctgatcat gacgaacgtt
     8161 tcgccctgaa gaagtgacat cattgcgggc gcgccgccct tgtagggaac atggatcatt
     8221 ttgacctgcg ccatcgcctg gaaaagctcg gtgccgagat gcgccggacc gccctgtccc
     8281 gtcgtggcat aggcgatacg gccggggctc gccttcgcca ggttgatcaa ttcgcgcacg
     8341 gtccttgccg gaagcgacgg atgcgtgacc agcatatagg cggtcgtgcc ggcgagtgat
     8401 accggggcga agtccttgat cggatcgtac ctccgatcgg cgtcgagggc gggccccgtg
     8461 atcagcgtgg ccgcgctccc gagcaacagc gtgtagccgt ccttcgggga acgagcaacg
     8521 aactcagcgg cgatgcgtcc gcccgcaccg ccgcggttgt cgacgatgat ctgctgaccg
     8581 agcgcggcgc tcaccttctc cgcgatgagc cgggcaaccg tgtcggtccc gccgccgggc
     8641 acgttcggga caatgagccg gatcggcttg gacggatagg tttgggcgtg cgacagcggt
     8701 ggcgcgaagc aaagccccat cgccgccgtc atgaatgaaa tgccgacaag agagctttgc
     8761 ttcatatcga atatgcgcaa gaatggctgt aacgggcgtt acggccaatg atgcgcgcgg
     8821 gggacgaagc gggtcaaatc caggacgccg cagtggctcc gggcgacgtc gggaacgaat
     8881 acagggtcag gtttcgcgtt gatgctaatt cctgcggatg agggaatcac gccttccacg
     8941 gcgacaggtt gtcgtgtcta acacaggcgc gcgcggattc cctcgaccat ttcgttcagc
     9001 gacgagaatg ccgtgacgcc cgcggccgcg agcgcggcgc ggtcgcggcg ttgcggacat
     9061 cctcaccaag cagacgctcg acccgctgcc catgtcgccg gagcagttcg cccagcgcct
     9121 caaggcggac tatgacaagt acgagaaggt gatccggatt tcgggcgcca ggatcgactg
     9181 accggatgaa tctcggaatc agcgggaaga ccgcgctcgt cgtcggcgcg agcgaaggcg
     9241 tcggctacga gagcacgaaa gggctgcggg aggagggtgc gcgtgttctc atttgctcgc
     9301 gcatcgccgg caagctcaaa tacaataact gatctgaggt tgccatggcc ctcaagtacc
     9361 ccctggtgca cgttgacgac gtccgtcccg agaacatgaa ggagggggag ggctgggcca
     9421 tctccgagtt ccggctgccc atctcgggca ggaacgggag ctctaccacg ctcttccact
     9481 ccatcttccg gcccggctcg acccatgcca ggcacctcca ccgcaactgc gacgagatcg
     9541 ccgtttatgt gaaaggacac ggcgccgtcg ggcaacggaa cgagcgcaca gaggtccgcg
     9601 cgggccactg ccgcctcatg cccaaaggcg cggagcactt cttcttcaac gagacgaaga
     9661 acgaggaaac ccttgtcatc ggtttctacg tcggcgcgaa gagcgttgcg gacaccggct
     9721 acgagtttct cgggcccgtg accgaggcgg acctcaggat gccccggcgc gagaacttca
     9781 gcgacggcgt tctggtccac gtcgacgacg tgaagccctc gcagatgaac gtgaaggacg
     9841 gatggagcat caccgacttc cgcatcccca ttggccgcca caacgggagc ccgaccgctc
     9901 tcttctgggc gaagttcatg cccggcgccg ttcacaagaa gcaccggcac gagaactgcg
     9961 aggagctcta ctacgtcatc cggggccacg gcctggcggg cgcgggcccg gaccgcgcag
    10021 aggtgcgggg cggccacgtc cactacatcc caaagggcgt cgagcacttc ctgtacaacc
    10081 tgagtgcgac cgagcccctc gaggccatcg gtatctacgt tggcgcgggg agcgtcgagg
    10141 agacgggcta cgcctacacg ggcgaggtga ccgaggcgga catcagggcc cggacggcgt
    10201 agctaacggg aacggctacc cggagctctc cacgtagcgc cggctaacgc aggcgagcgc
    10261 ggattccctc gaccatttcg ttcagcgacg agaataccgt gacgcccgcg gcgcccttgg
    10321 cggtataggt tccgtggctg ccgtgcacca gcgcgctgaa agaatccgct accacgcagc
    10381 tttgagtatc tcgagaagct cagtgacgtc cgtgacgtgc cgcggattct gatggaggaa
    10441 ccagtcgacc gcggcgtcct gcgcgagctg cggaagcgca tcctccggga tgccgacgtc
    10501 gcgtagccgc ttcggcatcc cgagccgagc gaaaagctcc tccgtcgcgc gcacggcgcc
    10561 agcaacggct gcatcggcgt tatcgggcac gccggcgccc aatgcctccg ccacgagcga
    10621 gagccggttg cccgtcgctg gcgcgttgaa ccgcatcgca tgcggcagca tgagcgcgtt
    10681 cgtgatcccg ttgttcaaat cgaagcgcgt gccgatgcaa tgggcgactg ccgatgtcag
    10741 gcctgacggc gcatggtccg tacctagccc ggaaaggagc gcgccgacca taagttgccc
    10801 gcgcgtctcg gcatcatccg gatccgccag caatccgggc agataacggc cgagaaggcg
    10861 caatgcgtga acaagcaggg catcggcaag cgggtcgcgg ttgcgcgatt caatcccctg
    10921 gacggccatc gagaaagcct ggactcctgc gcccaacgct aactgcggtg gtgcggttag
    10981 tgcaagctct ggatgaatga ataacgccga cgtgcgcgtt tttgggtcga acagggcgag
    11041 gcgttgaccg gaggcagggt ccacaactgc gctgccggct ttcccgtagg cggtagtagg
    11101 ggttgtcgcg accacgaact ggggaagctt gggccgttgc aacttgggac tgagcggtgg
    11161 tttgccattc cggtactggg tgcacagttc gtgtatgtcg cgcgcttcgg ccaggaggat
    11221 gctcgaggcg cgcgcggtga cgacggccga gccgccgccg acggcgatga tggcgtcggc
    11281 tttcagcgct cgtagtacct cgacgccggc cagcaccgcc ggcagcggac tgtgcgcttt
    11341 gacgccgtcg aatacgccgg catatcgatc gcccagtgcc tctgtaacgg tacgcagacc
    11401 ttcagttcga tgcgcgagcg tctggccgca gaagacgact gtgcggcggc aggcgagtcg
    11461 gtcgagctca gcgggtaatt gctgcaggct gtccgcgccg tagtacacgc ggcagggtgg
    11521 cgtgacgtgg cgaaacgatc tcacgtgcgt gaatgaggct cccttgacgg ggcgtcagct
    11581 cgagacggcg cccgagagcg catccgccag cggccgcacg tcagacaggc tgtcgatttc
    11641 catgatcgcg tcggcgagct ccgcggcttg tccatcgccc accttcgcgt agcgcaggtt
    11701 cgctttgaat ttcgcgagca gctcgtcgtc gctgagcgga ttgtcgggat gccccttcgc
    11761 gctcgtctgt tccacttcgc gcgtgccgaa cgcgccgtgc gcgcggagct tgcagccgcc
    11821 gacatcgcgc tgcgcggcag tgggatcggg cgtaccgctg attttcccga tccattcgcg
    11881 aagcttcggc gacggcggaa tttcttcggc gagctgccgc ggcgtcactt cgccgtaggc
    11941 aagtgcgctc gctacggacc agtagacgct gaactgcgcg ttgacgcgcg tctgcggcgt
    12001 gcgcttctcg gatacgtcgc ggccgacgac gtcgtgcgcc tgcggaccga tcacgagctc
    12061 gacgcgctcg attttttcag tgtcgcggcc aagctgcgcg cggagatcga gcgccgcgct
    12121 gatcgcagga tgcgtgaggc ggcacgtcgg gtacggcttg atcgagaggc ggtcgaattc
    12181 ccattcggtg ccgagcccgc gtgtcgcgcg cgcgggatcg aaaagatcgt gcagatacac
    12241 gcggctcaga ccgtcctcgc ccgtgagcgg atgtttgacg ccgggcaatc cgccgcttgc
    12301 catcagcgca gccgtgatcg cattcgctgc ggcgaagcca ggctgcacgt gcttcgtcgg
    12361 cgcgccttca cgcgtcgatt gatggttgcc gctcgcgagg cagtaaacga tgccgagcgc
    12421 gtttcgcagc acctccgctc gctgatcgac gagacgcgcg gcggcgagcg ttgcgccgaa
    12481 atggccgaag agcgcggtgt agatccagcc gccttcgacg agattgagct tcgtcgcgac
    12541 acccagccgg cacgtgagct cgatgccgag cagcacggct tcgcagaatt cccttccgct
    12601 cacttcgccg cgcagtcccg acgcggcgaa cgcggccggt atggccgccg agccggcgtg
    12661 aaggacggct tcatcatgcg tatcgtcgaa ctccagcaca tggccgaagt agccattgca
    12721 gagcgccgcc gtcggcgccg gcacgcgtgg tccaccgatc acgcgcgcat cgggccggcc
    12781 gcccatatca atcagaactt cgagccatcg cgggagcccc gccaccgcac ggccggcgat
    12841 cgcggcgccg agataatcga gcagctcgcg cctgacgctt gcacgcgtgg cttgcggcac
    12901 ctgctcccag cgcagcgctt gcgacaagtc gatgaatgcg tcgagcgtct gctccgacgc
    12961 atcgactgcg atttcgtgtg aacccataga tcggctctcc gctttcgcta ttccgcgcgt
    13021 atccccgcct gtttcgcgat gcggccccac ttcgcgagat cgttcacgat gagcttgccg
    13081 aatgcctccg gggtggtgat caccggctcg gcggcttcgg ccgagaggcg cttcgccatc
    13141 tccggctcgc gcaggattgc gttgatctcg ccgttgagct tgttcacgat cggccccggc
    13201 acacccgcgg gcgcgagcac gccccaccag atgctgccgt cgtatcccgg aacggtttcg
    13261 tcgatcgccg gcacctgggg gagcaaagcc gagcgcttcg gtgagccaac gccgagcggc
    13321 ttcagttttc ccgagcgaat gtggggcagc gcctggatca gcgagccgaa cacgacttcg
    13381 acttggccag tcatgacgtc gagcatcgcc gggccgccgc ccttgtacgg gatgtgaatc
    13441 agtttcactt tcgcgagctg attgaagagt tcggcagaaa agtggttgaa gccgccggtg
    13501 ccggtcgatg cgtagcgcag ctcgtcgggt ttcgctctgg cgagtgcgat caactgtttg
    13561 acggtctgcg ccgcgaagtt cggatgcgag caaatggcgt tcggtccggt ggcgacttgc
    13621 gcgatcggcg cgagcgattt gacgggatcg aatggcagct tttgtatcgc tggaatctgc
    13681 gcgtaagtcg tggccaccat gaccagcgta tggccgtccg cgggtgcacg cacggcaaga
    13741 tccgtcccga tggcaccgga tgcgcccggc cggttgtcga cgatcgtttg ctggcccagg
    13801 cgctcgctca tcttatgtgc gacgaggcgc gcgagaatgt cgttgctgcc accgggcggg
    13861 aagggcgaga tcatgcggat cggcttggtc ggaaagcgga ttgcatccgg agtctgcgcg
    13921 gcgccggttc cagcgacgga aacgcagaac agggcgagcg cacagagagg gagtcgttgg
    13981 atgggagctt tcatggtcat cctcggggaa atcgcacttc agggtaatgc tattcggcgc
    14041 gtatgccggc ttgcttcgcg atacgcaaat acttcgcgat atcgttcacg atgagcttgc
    14101 caaacgcttc gggcgtgctg acgacaggct cggccgcttc ggcaacgaga cgcttagcca
    14161 tctcgggctc gctcacaatc gtgccgatct cggtgttgag cagcgtgacg attgcgccgg
    14221 gaatgcccgc gggacccagt acgccccacc agaccgagcc gtcgtagccc ggcacgccgc
    14281 tttcggacac ggtgggaacg tccggcagga gcggagaacg cttggccgac ccgacgccga
    14341 gcgccttgag cttgcccgag cggatgtggg ggatcgcctg gatcagcgtg ccaaacacca
    14401 cctccacctg ccccgtcatc acgtcgagca tcgacgggcc gccgcctttg taaggaatgt
    14461 gcacgagcct gaccccggca agcatgttga attgctcgcc gccgaaatga ttgaatccgc
    14521 ctatgccgga cgatgcgtag cggatttgcc ccggcttcgc tttcgccagc gcgaccagat
    14581 ccttcgcggt tctcgcgggg aagttcgggt gcgtggcaat gacgttcccg cccgatgcga
    14641 tctgcgcgat gggcaccagc gacttaagcg ggtcgtacgg gagtttgtgt accgcgggat
    14701 tcatcgtgta ggacgtcgac acgatgagca gggtatgccc gtcggcggga gaacgcgacg
    14761 cgagctcggt gccgatgatg ccgtcagcgc ccggccgatt gtcggcgatg gtggttttgc
    14821 ctaggcgctc ggtcatcttc tgggcgataa agcggccgag gatgtcgttg ctgccgccgg
    14881 gcggaaacgg gatgatgagc cggatgggcc gcgacgggta cttggacaca tccaccggct
    14941 gggccggcgc gtgatcggcg tgcgtgccca gcgccagcac cgccgcgcct gcggcgagcg
    15001 caactgcgaa ttgggacatc ttcatggtcg tcctcgggga actaaggagt gcgcagttta
    15061 agctgtctgc cgttagattg tcaaatcatt tagatcattt gacagatttg gacagacgga
    15121 tgtaggatcg tggtcatgcc cattcgatcc ggccccagca gcacgctcga tttctcccgt
    15181 cacgtccgcg cagacgacgt ggtgacgtgg ccccagggga cgggcgagcc gttgggcctc
    15241 acgcgccgtc tcgtcgagca gcgccacgca ctgccgccgc tcgagctgtt tgtcggcatg
    15301 atgacgagca agacgctcaa gcccgagcac gccgatcgct ttcgctttcg tggtctcaat
    15361 ggcgccggca cgacccgagc gctcaccact gcgaatctgc tcgacatcat tcctgcgtat
    15421 gtgtccgccg tgcccgcgct catccgctcg cgcgcgattc gcgtcgacgt cgtgcttctt
    15481 cgcgcgcggc cgcatccgaa gcccgggttc ttcaccgtgg gggtcatggc ggatttcatt
    15541 cccgcaatgg tgcaagcggc gcgctgcgtg atcgcggaga tcgacgagcg cctgccagtg
    15601 acatcgcagg atgcgctggt cgccgaagca gacatcgacg tgcttaccga gtgcgatgcc
    15661 gatgagattc tcctgccgga tcccgagccg gatgagctcg ataagcgcgt cgcggcgcac
    15721 gtcgcggctc tcattcccga cggcgcgacg atccaggttg gtattggcgg tttaccggtc
    15781 gcggtttgcc gcgcgctcga gaatcatcgc gatctcggcg tgcacagcgg cgtcatttcg
    15841 gatgtcgtcg tggatctcgt cgagaaaggc atcgtgacca atgcgcgcaa gggaatcgac
    15901 gccggccgca tcgtcaccgg gggtctcttc gggagccgcc gcctgatgga tttcgccgac
    15961 ggcaacgacg tgctcgagat gcgctcgtcc gaatacaccc acagccagca ggtcctcgcg
    16021 cgcgtgcaga gcctgtacgc gatcaactcg ggcgtcgagg tcgatcttac cggtaacgtg
    16081 aactccgagg ttgcggccgg ccgctatctc ggcgccatcg gtggacagcc ggacttcgtc
    16141 cgcggcgcat tcgcgtcgcc cggcggccgc gcgatcatcg cgctgccgtc gacgacaccc
    16201 gacggcaagc actcgcggat cgtcgcgtcg ctcgaaggcc ggccggtcac gactacgcgc
    16261 gcggacgtcg acatcgtcgt caccgagtac ggcgtcgccg atttgcgcgg acgctccttc
    16321 agcgagcgcg cggaacgcct ccgcgccatc gcgcatcccg atttccggga cgatctcgtg
    16381 aaatcaatct cgaaagcagc gtaaatgcag ccaggcacga aaacgaatcg agccgattcc
    16441 ggcatcgtcc tcgagcgaag cggcgagggc atcgttcaga tccgcctgaa tcgtcccgag
    16501 cgcctcaacg cgctcggtat ggacatggtg gaggcgttcc aggcggcgat cgcggacgcg
    16561 atcgccggtc gcgcgcgcgt ggtgctcgtt cgcggcaccg gccgcgcgtt ctgcgccggg
    16621 gcagacctga aggagcgccg gacgatggat gaagcggcgc gcgtaagaca caaccgggcg
    16681 atcaatgcgg cggtcgacgc gctcgcggca gccccgatgc cgacgatcgc cgtcatcaac
    16741 ggcctcgcga tgggcggcgg ctgcgaaatc gcgctcgcct gcgacctgcg ctacgccgcg
    16801 gaggacgcgc agatcgggct caccgaggcg cggatcggcg cgattcccgg cgccggcgga
    16861 acgcagcgct tgccgcgcgt gattggcgct tcgcgcgcgc tcgagcttat tctcaccggc
    16921 gagcccgtca ccggcaaacg cgccgaggag atcggtctcg tgaacgggtc cgtcgccgcg
    16981 gacaagctcg acgcgcacgt catgagaatc gcaacggtgc ttgcatcgcg ttcgccgacc
    17041 ggcgcgcaga cgaccaagcg cctcgtctac cgcggcctcg aaatgacgct cgccgacgga
    17101 ctcgtgcagg aaggcctcgc gcttcgcgag atcctcgctt cacccgacta tgcggaaggt
    17161 ctcgccgcgt tcgccgagaa gcggcagccg cgctttggcg gaggtacgat tacatgagcc
    17221 gcgcagccag cacccatcgc agcaaagccg agcacgtcgc gcagcagctg ctcgagcgca
    17281 tcattgccgc gaagttgaag cccggcagca gcttcgcgac cgaagccgag ctcctctcgc
    17341 agttcaatgt gagccggccg acgctgcgcg agagcctcaa gcttctcgaa tc
//
LOCUS       DBNGWZ010000618         7580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3375086, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000618 DBNGWZ010000000
VERSION     DBNGWZ010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3375086"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /gene="prpC"
                     /locus_tag="AC16CX_19285"
     CDS             complement(<1..1050)
                     /gene="prpC"
                     /locus_tag="AC16CX_19285"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019543 - propionate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HIR3014686.1"
                     /translation="MSNTTPSPVQKPKKSVALSGVAAGSTALCTVGRSGNDLHYRGYD
                     ILDIADQCEFEEIAHLLVHEKLPTRAELAGYKDRLKALRGLPAPVKAVLEQIPASAHP
                     MDVLRTGCSTLGVILPEEDDHNIEGARGIADRLMACFGSMLLYWHHYSRDRRRIEVET
                     DDDSIGGHFLHLLHGRPAKPLWVKAMHTSLNLYAEHEFNASTFAARVVAGTGSDLYSA
                     ITGAIGALKGPKHGGANEVGFEVMSRYETPDAAERDITARVRNREVVIGFGHPVYTIA
                     DPRNKVIKEVARRLSQDAGEMEMFEIADRIERVMMREKKMFANLDWFSAVSYHRMGVP
                     TAMFTPIFVIARTSGW"
     gene            complement(1151..2140)
                     /locus_tag="AC16CX_19290"
     CDS             complement(1151..2140)
                     /locus_tag="AC16CX_19290"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR3014687.1"
                     /translation="MKRPVRVAVTGAAGQIGYSLLFRIASGELLGRDQPVILQLLEIP
                     DEKAQKALKGVMMELDDCAFPLLHGMVPASDPVVAFKDAEVALLVGARPRGPGMERKD
                     LLEANGKIFAPQGRALSEVASRKVKVLVVGNPANTNCLIAMKNAPGLKPSNFSGMMRL
                     DHNRAVGQVAQKLGKPVSSIRKMTVWGNHSATQYPDVFHATADGKKAWPMINDPAWLE
                     NQFIPTIQKRGAAIIEARGLSSAASAANAAIEHVRDWIRGTRAGDWVSMGIPSEGSYG
                     VPEGVIYGYPVTCKNGQYRIVKGLEVSEFSRGRMMATLKELHEERDSIKHLLG"
     gene            2418..2792
                     /gene="sdhC"
                     /locus_tag="AC16CX_19295"
     CDS             2418..2792
                     /gene="sdhC"
                     /locus_tag="AC16CX_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR3014688.1"
                     /translation="MPKTRPKYLNLIQIRLPVPGLISIMHRVSGAVLFLFLPFLLTLF
                     EMSLESARTFARFKGVFSHWMVKLILIGLLWAFLHHLCAGIRHLALDLDYGTELPAAR
                     ASSMAVLAVSIVLTVVAGVLIW"
     gene            2786..3133
                     /gene="sdhD"
                     /locus_tag="AC16CX_19300"
     CDS             2786..3133
                     /gene="sdhD"
                     /locus_tag="AC16CX_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HIR3014689.1"
                     /translation="MVKREVVGAHYGLRDWLAQRVTAVIMVVYTMLIVSAAIGSPHLD
                     YWTWRTLWQVPVVRYATMLFVVSLLLHAWIGMREIFMDYVKDPGLRLALYVLVIGALV
                     WYGVWAVQILWSL"
     gene            3134..4900
                     /gene="sdhA"
                     /locus_tag="AC16CX_19305"
     CDS             3134..4900
                     /gene="sdhA"
                     /locus_tag="AC16CX_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR3014690.1"
                     /translation="MALPVRQFDAVIVGGGGSGMRAALELARADLKVAVLSKVFPTRS
                     HTVAAQGGVAASLGNIDEDNWHWHMYDTVKGSDYLGDQDAIEFMCRKASEVVYELEHF
                     GMPFDRLAGGKIYQRPFGGHMREYGKSPVPRACAAADRTGHALLHTLYQANLKARTQF
                     FVEWMALDLIRDREGAVLGVTALEMETGEVMILQAKATVFATGGAGRIFAASTNAFIN
                     TGDGLGMAARAGIPLQDLEFWQFHPTGVAGAGVLITEGVRGEGGYLLNKNGERFMERY
                     APNAKDLASRDVVSRAMATEIKEGRGAGKDGDYILLKLDHLGAETISKRLPGIREIAK
                     KFANVDPVKDPIPVVPTCHYQMGGIPTNYHGQVVVPANGNPETVVPGFYAAGECSCVS
                     VHGANRLGCNSLLDLLVFGKAAGEKVIRDVGTDGRAHRNLPQDAGELTLSRLARLDAQ
                     KNGERVADVGADMRRIMQLHCGVFRFPDLLTEGVKKIKEVAERAGHTEISDKSRIFNT
                     ARVEALELDNLVETAVASLVSAEARKESRGSHDRADYHQRDDANWLKHTLWYKQGSRL
                     EYKPVHTKPISVEPFEPKARVY"
     gene            5019..5714
                     /locus_tag="AC16CX_19310"
     CDS             5019..5714
                     /locus_tag="AC16CX_19310"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR3014691.1"
                     /translation="MRFSIYRYNPESDEKPRIQAYDITLEPTDRMLLDALVRIKSHDD
                     TLVFRRSCREGVCGSDAMNINGRNGLACITRLADLKEPVEIRPLPGLPVIRDLIVDMS
                     QFFKQYHSVKPYLVNNDPPPEKERLQSPEDREELDGLYECILCACCSTSCPSFWWNPD
                     KFVGPAGLLQAYRFIADTRDQATNERLDNLEDPYRLFRCHTIMNCVEVCPKGLNPTRA
                     IGKIKDLLVKRTV"
     gene            5727..5957
                     /locus_tag="AC16CX_19315"
     CDS             5727..5957
                     /locus_tag="AC16CX_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006105 - succinate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0034552 - respiratory chain complex II
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HIR3014692.1"
                     /translation="MGDLDRIRWRCRRGLLELDLVLEGFLEHRFDRLDAEQRRLFDEL
                     LDRPDNDLLDLALGRCEPAPRYRTVVEMLRAG"
     gene            6027..7325
                     /gene="gltA"
                     /locus_tag="AC16CX_19320"
     CDS             6027..7325
                     /gene="gltA"
                     /locus_tag="AC16CX_19320"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3014693.1"
                     /translation="MAEKLATLTFSDGTPPVDFPVLTGAVGPDVIDVRALYGKTGKFT
                     YDPGFMSTASCRSSITYIDGDKGVLQYRGYPIEQLAEHCSFLEVAYLILNGELPSNKR
                     LDEFVHLVTHHTMVHEQLIRFYSGFRRDAHPMGVMCGVVGALSAFYHDSINIYDARSR
                     EISAFRLIAKMPTITAMTYKYNAGQPFMYPKNALSYVDNFLYMMFGTPCEEYRPNPVL
                     VRAMDRILILHADHEQNASTSTVRLAGSSGANPFACIAAGIASLWGQAHGGANEAVLK
                     MLGEIGDANNIPGFIKKVKDKQDHAMLMGFGHRVYKNYDPRAKIIQKTCHEVLEELDL
                     KDDKLFKLAMALEKVALEDEYFIKRKLYPNVDFYSGIVYKALGIPVAMFTAIFALART
                     VGWIAQWNEMITDPDQKIGRPRQLFAGATRREFVPMNKRK"
     gene            7455..>7580
                     /locus_tag="AC16CX_19325"
     CDS             7455..>7580
                     /locus_tag="AC16CX_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 subunit family
                     protein"
                     /protein_id="HIR3014694.1"
                     /translation="MMKQMLGNSYLFGANAPFIEALYDAWLKDPGSVEPRWRDYFD"
BASE COUNT         1347 a         2410 c         2461 g         1362 t
ORIGIN      
        1 ccagccgctg gtgcgggcga tgacgaagat cggcgtgaac atcgcggtgg gaacgcccat
       61 cctgtggtag gagaccgcgg agaaccagtc gaggttcgcg aacatcttct tctcgcgcat
      121 catcacccgc tcgatgcggt cggcgatctc gaacatctcc atctcgcccg cgtcctgcga
      181 gagccgtcgc gccacttcct tgatcacctt gttgcgcgga tcggctatgg tgtagaccgg
      241 gtggccgaag ccgatcacga cctccctgtt gcggacgcgc gccgtgatgt cgcgctccgc
      301 cgcatcaggt gtttcgtaac ggctcatcac ctcgaacccg acctcgttgg cgcccccgtg
      361 cttggggccc ttcagcgccc cgatcgcgcc ggtgatcgcc gagtacaggt cggagccggt
      421 gccggcgacg acgcgcgccg cgaaggtgga ggcgttgaac tcgtgctccg catagagatt
      481 gagggaggta tgcatggcct tcacccacag cggcttcgcc ggtcttccgt gcagcaggtg
      541 caggaagtgt ccgccgatcg aatcgtcgtc ggtctcgacc tcgatgcggc gccggtcgcg
      601 gctgtaatga tgccagtaga gcagcatcga gccgaagcag gccatcaggc ggtcggcgat
      661 gccgcgcgcg ccctcgatat tgtgatcgtc ctcctccggc agtatcacgc cgagcgtcga
      721 gcaaccggtg cgcagcacgt ccatcgggtg cgcggaagcg gggatctgct cgagcaccgc
      781 tttcacgggc gctggcagac cgcgcagggc tttgagccgg tccttgtatc ccgcgagctc
      841 cgcccgggtg ggaagctttt cgtgcaccag caggtgcgcg atttcctcga actcgcattg
      901 atcggcgatg tcgaggatgt cgtagccgcg gtagtggaga tcgttgccgc tgcgcccgac
      961 ggtgcacagc gcggtgctgc cggcggcaac gcccgacagc gccaccgact tcttgggttt
     1021 ctggacaggg gacggcgtgg tgttgctcat cgcttatcct tcggccggta agcagtaagc
     1081 ggttagcggt aagccgtaat cgctagacaa gttcacccgc tttcggctca cagctcaccg
     1141 cttaccggcg tcagccgagc aggtgcttga tcgaatcgcg ctcctcgtgc aattccttca
     1201 gcgtcgccat catgcggcca cggctgaatt cgctcacctc caggcccttg acgatccggt
     1261 actgtccatt cttgcaggtc acggggtagc cgtagatcac gccttccggt acgccgtagc
     1321 tgccttcgga cggtattccc atgctcaccc agtcgccggc gcgcgtgccg cggatccagt
     1381 cgcgcacgtg ttcgatggcg gcgttggccg cgctcgccgc cgaggacagt ccccgtgcct
     1441 cgatgatggc cgcgccgcgt ttctggatgg tcggtatgaa ctggttctcc agccacgccg
     1501 ggtcgttgat catgggccag gctttcttgc cgtcggccgt ggcgtgaaag acgtcgggat
     1561 actgggtcgc cgaatgattg ccccagacgg tcatcttcct gatgctcgaa accggttttc
     1621 cgagcttctg ggcgacctgg ccgaccgccc ggttgtggtc gaggcgcatc atgcccgaga
     1681 aattcgacgg cttgagcccc ggcgcgtttt tcatggcgat caggcagttg gtgttggcgg
     1741 gattgccgac caccagcacc ttgaccttgc ggctcgcgac ttcggagagc gcccggccct
     1801 gcggcgcgaa gatcttgccg tttgcttcga gcagatcctt gcgttccatc ccggggccgc
     1861 gcggccgggc gccgaccaac agcgcgacct cggcgtcctt gaacgccacc acggggtccg
     1921 aagcggggac catgccgtgc aacaggggga aagcgcagtc gtcgagttcc atcatgacgc
     1981 ccttcagcgc cttctgcgcc ttttcatcgg ggatttcgag cagctggagg atgaccggct
     2041 ggtctcttcc gagcaattcg ccgctggcga tgcggaacag caggctgtag ccgatctggc
     2101 cggcagcacc ggtgacggcg acgcggacgg gtcgtttcat gaagtggact cctgaatcgc
     2161 ggaaagtgat attgtgtttc gggaaggagg cgcggcggga cgggacgttg cgcgcgcgtg
     2221 ctcgatcgcg tggcgcgatg atagccaagg catgcccgag gtgacaagcg agctccggaa
     2281 atcgcgcatc gcatgaaggt tcacgacctg gacgccggat caggcagcca agggcacggc
     2341 ttatgcagtg cggcgaaaac tgctaagatt tcaaccatca atcgttgtga atcgctttcc
     2401 cgccaacctg acttttcatg cctaagacgc ggccgaagta tctgaacctg atccagatcc
     2461 ggctgcccgt gcccgggctg atctcgatca tgcaccgggt cagcggggcg gtcctgttcc
     2521 tgttccttcc cttcctgctg acgctgttcg agatgagcct cgaatcggcc cggaccttcg
     2581 cccggttcaa gggcgtgttc tcgcattgga tggtcaagct gatcctgatc gggctgctgt
     2641 gggccttcct gcaccacctg tgcgccggta tccgccatct ggcgctcgac ctggattacg
     2701 gcaccgagct gccggcggcg cgcgcgagca gcatggcggt cctcgcggtc agcatcgtcc
     2761 tgaccgtcgt cgccggagtg ctgatatggt gaagcgcgag gtcgtcggcg cccattacgg
     2821 gctgcgagac tggctcgcac agcgggtcac cgccgtgatc atggtggtgt acacgatgct
     2881 catcgtgtcc gccgcgatcg gctcgcctca cctggactac tggacctggc ggaccttgtg
     2941 gcaggtgccg gtggtgcggt acgcgacgat gctgttcgtc gtgagcctgc tgctccacgc
     3001 ctggatcggc atgcgcgaaa tcttcatgga ctacgtcaag gacccgggac tgcgcctcgc
     3061 gctctacgtg ctcgtgatcg gggccctggt gtggtacggc gtgtgggcgg ttcagatact
     3121 ctggagcctg tgaatggcac tgccggttcg acagtttgac gcggtgatcg tcggtggcgg
     3181 cgggtcgggc atgcgcgccg cgctcgagct cgcgcgcgcc gacctcaagg tcgcggtgct
     3241 gtcgaaggtg ttcccgacgc gttcgcacac ggtggcggcg cagggcggcg tggcggcctc
     3301 gctcggcaac atcgacgagg acaactggca ctggcacatg tacgacaccg tcaagggttc
     3361 cgactatctc ggcgatcagg acgcgatcga attcatgtgc cgcaaggcga gcgaggtggt
     3421 ctacgagctg gagcacttcg gcatgccgtt cgaccgtctc gccggcggca agatctacca
     3481 gcggcctttc ggcggccaca tgcgcgaata cggaaaatcc ccggtgcccc gcgcctgcgc
     3541 cgcggccgac cgcacggggc acgccttgct gcacacgctt taccaggcga acctcaaggc
     3601 gcgcacgcag ttcttcgtcg agtggatggc gctcgacctc atacgtgacc gggagggcgc
     3661 ggtgctcggc gtcaccgcgc tcgagatgga aaccggagag gtgatgatcc tgcaggccaa
     3721 ggccacggtc ttcgccaccg gcggcgccgg gcgcatcttc gccgccagca ccaatgcctt
     3781 catcaacacc ggcgacgggc tgggcatggc ggcgcgcgcc gggatcccgc tccaggacct
     3841 ggagttctgg caattccacc cgaccggggt ggccggcgcc ggcgtgctga tcaccgaggg
     3901 cgtgcgcggg gagggcggct acctcctcaa caagaacggc gagcgcttca tggaacgcta
     3961 tgcgccgaac gccaaggacc tcgcctcgcg cgatgtggtt tcgcgcgcga tggcgaccga
     4021 gatcaaggaa ggccgcgggg cgggcaagga cggcgactat atcctgctca agctcgacca
     4081 cctcggcgcg gagacgatca gcaagcgcct gcccggcatc cgcgagatcg cgaagaaatt
     4141 cgccaacgtg gacccggtca aggatccgat cccggtggtg ccgacctgcc actaccagat
     4201 gggcggcatc ccgaccaact accacggcca ggtggtggtg cccgcaaacg gcaatccgga
     4261 aacggtcgtc cccggcttct acgcggccgg cgaatgctcc tgtgtatcgg tgcacggcgc
     4321 caaccgtctg gggtgtaact cgctgctcga cctgttggtg ttcggcaagg ccgccggcga
     4381 gaaagtgatc cgggacgtcg ggacggacgg gagggcgcat cgcaatctcc cgcaggacgc
     4441 gggtgagctt acgctctcgc gtctcgcgcg tctcgacgcc cagaaaaacg gggagcgcgt
     4501 ggcggacgtg ggcgccgaca tgcggcgcat catgcagctg cactgcggcg tgttccgctt
     4561 cccggacctg cttacggagg gcgtgaaaaa gatcaaggaa gtcgcagagc gcgccggcca
     4621 caccgaaatc agcgacaaga gcaggatctt caacacggcg cgggtggagg cgctcgagct
     4681 cgacaacctg gtcgagaccg ccgtagcctc gctggtttcc gcggaagcgc gcaaggaatc
     4741 ccgcggctcg cacgaccgcg ccgactacca ccagcgcgac gacgcaaact ggttgaagca
     4801 cacgctgtgg tacaagcagg gaagccggct ggagtacaag ccggtgcata cgaagccgat
     4861 ctcggtcgag ccgttcgagc ccaaggctcg ggtttattaa tagtgactcg tttctcgtta
     4921 ttcgttgatc gccaacctcc taatagtgcg ccggtttacg cgaaaatgag acgagaaacg
     4981 agtaacgagc aacgagtcac gcttttgtgg agcagctgat gcgattctcg atctaccgct
     5041 acaaccccga gtccgacgag aagccgcgca tacaggccta cgacatcacg ctcgagccga
     5101 ccgaccgcat gctgctcgac gcgctggtgc gcatcaagtc gcacgacgac acgctggtgt
     5161 tccggcgctc ctgccgcgag ggcgtgtgcg ggtcggacgc catgaacatc aacggcagga
     5221 acgggctcgc gtgcatcacg cggctcgcgg acctcaagga accggtggag atccggccgc
     5281 tgccggggct gccggtgatc cgcgacctga tcgtggacat gtcgcagttc ttcaagcagt
     5341 accattcggt caagccctac ctcgtcaaca acgatccccc gccggagaag gaacggctgc
     5401 aatcgccgga ggaccgcgag gagctggacg ggctctacga gtgcatcctg tgcgcctgct
     5461 gctcgacttc ctgcccctcc ttctggtgga atcccgacaa attcgtcggg ccggcggggt
     5521 tgttgcaggc ttaccgtttc atcgccgaca cgcgcgacca ggcgaccaac gagcggctcg
     5581 acaacctgga agatccgtac cgcctgttcc gctgccatac gatcatgaac tgcgtggaag
     5641 tgtgcccgaa ggggctcaac ccgacacggg cgatcggcaa gatcaaggat ctgctggtca
     5701 agcgcaccgt ctgaacgggc cgatgcatgg gtgacctgga ccggattcgc tggcgctgcc
     5761 gccggggact gctcgagctc gatctcgtgc tcgagggttt cctggagcac cgcttcgacc
     5821 ggctcgacgc ggagcagcgg cggttgttcg acgaactgct cgaccggccc gataacgatc
     5881 ttctggacct ggcgctgggt cgctgcgagc cggcgccgcg ctaccggacg gtggtggaaa
     5941 tgctgcgcgc gggctgaggc agccccgggc gcgcggggtg tggaacggcg cgagccgacc
     6001 gattataaaa acctgcggag accaacatgg cagaaaagct ggcaaccctg accttcagcg
     6061 acggcacgcc gccggtcgat ttccccgtgc tcaccggcgc cgtgggaccc gatgtgatcg
     6121 atgtccgcgc gctctacggc aagaccggaa aattcaccta cgacccgggc ttcatgtcca
     6181 cggccagttg ccgctccagc atcacctata tcgacggcga caagggcgtc ctgcagtacc
     6241 gcggctatcc catcgagcaa ctggccgaac attgcagctt cctggaagtc gcgtacttga
     6301 tcctgaatgg cgagctgccc agcaacaagc gactcgacga gttcgtgcac ctcgtcaccc
     6361 accacaccat ggtgcacgag cagctcatac gcttctattc gggcttccgc cgcgacgcgc
     6421 acccgatggg ggtgatgtgc ggcgtggtgg gggcgctgtc cgcgttctac cacgactcga
     6481 tcaatatcta cgacgcgcgc agccgggaga tttcggcgtt tcgcctgatc gccaagatgc
     6541 ccacgatcac ggcgatgacc tacaagtaca acgccggcca gccgttcatg tacccgaaga
     6601 acgcgctgtc ctacgtggac aacttcctct acatgatgtt cggcacgccg tgcgaggagt
     6661 acaggccgaa tccggtgctg gtacgcgcca tggatcgtat tctgatcctc cacgccgacc
     6721 acgagcagaa cgcttcgact tcgacggtgc gcctggcggg ttcgtcgggc gcaaatccct
     6781 tcgcctgcat cgccgccggc atcgccagcc tgtggggcca ggctcacggc ggcgccaacg
     6841 aagccgtgct caagatgctg ggagagatcg gcgatgcgaa caacattccc ggcttcatca
     6901 agaaggtcaa ggacaagcag gaccacgcca tgctgatggg cttcgggcac cgggtctaca
     6961 aaaattacga cccgcgcgcc aagatcatcc agaagacctg ccatgaggtg ctggaggagc
     7021 tcgacctcaa ggacgacaag ctgttcaaac tcgcgatggc gctggagaag gtcgcgctcg
     7081 aggacgagta cttcatcaag cgcaagctct accccaacgt ggatttctac tcgggcatcg
     7141 tttacaaggc gctcggcata ccggttgcca tgttcaccgc gatcttcgcg ctcgcgcgga
     7201 cggtgggctg gatcgcgcag tggaacgaga tgattaccga tcccgaccag aagatcggcc
     7261 ggccgcggca gctcttcgcc ggggccacgc ggcgcgagtt cgtgcccatg aacaagagga
     7321 agtgacgagt gtcgggtgac gagtgacgag tgtcgggtga cgagtgtcga aggacaaggg
     7381 cgcagccaga gttttcactc gacactcaat cctcgacact cagtcctcga cactcagcac
     7441 cgcgaggtga tgtcatgatg aagcagatgc tcggcaactc gtatctcttc ggcgccaacg
     7501 cgccgttcat cgaggcgctc tacgatgcct ggctcaagga cccgggctcg gtcgagccgc
     7561 gctggcgcga ctatttcgac
//
LOCUS       DBNGWZ010000619        15886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_102090, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000619 DBNGWZ010000000
VERSION     DBNGWZ010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_102090"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="AC16CX_19330"
     CDS             <1..836
                     /locus_tag="AC16CX_19330"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR3014695.1"
                     /translation="YCYARPTHAYRNLSPGIDWETRLFAKVNAAEKLREELSRPGYRC
                     EVISLGANTDPYQPIERNFRITRGILEVCAEYNQPVGIVTKNAMVERDIDILAPMARK
                     NLVAVFISCNNLDHDLARRLEPRCSAPARRLQAMQRLSGAGIPVGVLVAPVIPFLTDH
                     QIEPVLEAAWECGARQAGYVLMRLPWEVKDLFKEWLERNYPLKARHVMSRVHAMRGGR
                     DNDPGFGSRMTGQGELARLLAQRFRKACERLGYNTEARNRELDTSQFRPPARPGQMAL
                     F"
     gene            874..1821
                     /locus_tag="AC16CX_19335"
     CDS             874..1821
                     /locus_tag="AC16CX_19335"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3014696.1"
                     /translation="MKNEVALYVGESLGKYGFPSGHPFGPDRQDAFWKEAVKQGLDQR
                     VAITDPRMAAEEELHRFHLPEFVAWVKERSEEGYGAIDYGDTPAYPGVYEAAATVAGS
                     ALHGLDTIMSGGALRTFQPIGGLHHARPGAAAGFCVFNDVGVVIDTLRDQYGVRRIAY
                     VDIDVHHGDGLYYPYEADPDLIYADIHQDGRTLYPGTGHAHETGKGAAQGTKLNIPLL
                     PGMGDREFLKVWEAVEAHLREFKPEFILFQCGADSLAGDPLAMLQYTPAAHAHAARRL
                     CALAGEMTGGRIMGFGGGGYHRGNLAAAWCAVLEEFLKA"
     gene            complement(2046..3458)
                     /locus_tag="AC16CX_19340"
     CDS             complement(2046..3458)
                     /locus_tag="AC16CX_19340"
                     /inference="COORDINATES: protein motif:HMM:NF017135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase domain-containing
                     protein"
                     /protein_id="HIR3014697.1"
                     /translation="MNRAASSPSPLSALERLRHWWQRLAATVFSAAGATAADRATARL
                     IERIKPLLEECRMQRGGEVSSRERAERIAAIYRATGASQRAAILNLITHEFAPNREEL
                     EQAIAAIQVAANDAELSRAEARLRIALDAPRAKFLAQFNLLPEGVKFLVDLRADLLAA
                     LPQEPALEVLKVELDGLLESWFDPGFLELQRISWQSPALVLEKLMTYEAVHPIESWND
                     LRNRLDTDRRCYAFFHPRLPNEPLIFVEIALAKGLPGSVQLLLDQSAPVQDPRTANTA
                     VFYSISNTQKGLRGVSFGNLLLKRVIEDLQLDLPRLHIFATLSPLPGFRKWLDQALAE
                     GRALLPAAETAKLAAALGYPDAGTALADALARPEWPADARLGEALREPMEKLCARYLL
                     QEKSGAHPLDSVAQFHLNNGARVSRIFWLADTSARGMRQSYGLMASYRYDLAEVDRNH
                     EQFSRDGHIAAARRVQRLLA"
     gene            3607..3756
                     /locus_tag="AC16CX_19345"
     CDS             3607..3756
                     /locus_tag="AC16CX_19345"
                     /inference="COORDINATES: protein motif:HMM:NF019389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HIR3014698.1"
                     /translation="MSRPPSEATPPRRPSRGPSASPRPRRRSATTAAGSGDERKQFMS
                     ACLKG"
     gene            3863..4633
                     /locus_tag="AC16CX_19350"
     CDS             3863..4633
                     /locus_tag="AC16CX_19350"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3014699.1"
                     /translation="MALFMRGVWNCLVALAGALVPAAGGMMDAQAQDIYEPQRRRMVE
                     EIAALTRETRFETGRASLSERVMAAMAKVPRHEFVPEGERRNAYANRPLPIGHGQTIS
                     QPYIVALMTDLMEVKPGDRLLEIGTGSGYQAAVLAELAVTVYTIEIVEPLAREAQERL
                     RRLGYRNVVAKAGDGYRGWPEHAPFDAIMVTAAPREVPQPLIDQLKPGGRLVVPVGTQ
                     IGGQSLLVIEKKLDGTVTRRNVLAVRFVPLTDKAGRQQ"
     gene            complement(4783..5760)
                     /locus_tag="AC16CX_19355"
     CDS             complement(4783..5760)
                     /locus_tag="AC16CX_19355"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014700.1"
                     /translation="MNGSLSRRFLITPFAAAILLFKPSVIPAQDYPAKPVRVIVPFAP
                     GGGSDITARQVAQKLTENLRQQFVVDNRGGASGLIGMELTVKSPPDGYTIMVMSGSFS
                     ATPATHKPAFDPINNIIPIAEVGYTPFVLSVHPSLPVKNAKELIALAKARPGQLNYVT
                     PGVGSLTHLATELLINMAKVSMVGIAYKSTGAALPDLLSGQCQLIVGSLLPTFPHFRS
                     GKLRPLAVTTSRRWYSLPDVPTLGQTLPGYEVELWFGVMAPRNTPRGIVEKLNATVNK
                     FLQDADMKKNLEAQGMVPSGGTPERFGERIRKDYDRWVKVVREQNIKPE"
     gene            complement(5867..8452)
                     /locus_tag="AC16CX_19360"
     CDS             complement(5867..8452)
                     /locus_tag="AC16CX_19360"
                     /inference="COORDINATES: protein motif:HMM:NF019410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HIR3014701.1"
                     /translation="MPNPDPPAAAQQASPFDKILEQSCDLVCERLDQALAGMLDKVEE
                     SLTALINETQNQETQVLYRETRDKVLAQRKSIEDSFRTRYLREFQTRSNRVRKIGQSF
                     SEFDLSALELDLVGEDDLDETLKFNNVAAKLRRFCDEELVALDQRVGVLLGDADLQAE
                     DNPFSPQAICDAYKYTCRQVDSNIKLRMVLLKLFDDHVVDAIRSIYKDVNALLVKNSI
                     LPKIRYGVSRQEGGKAPAAGAHADDEAPGTEKKAADKAAGSEQDFFSVLQSLLAKNPQ
                     ATAQLGAAGSVAVPGAGGVQVVLQGADLLNSLTRIQQGNVSAMTASDGQTVALPALGG
                     EAGTTNVLRELKSSNVGAGMGQMDAMTLDIVAMLFDQLFDEPKIPISVKGLIGRMQIP
                     MLKVAITDKAFFSQKTHPARQLLNTLGEIAVRLPAEFNSSSPLFGRLETIIQELMNGF
                     QDNLEIFDTTRERLQALIAEEDKRAEQETEATAKRIEQQESLALAKATAQDEIKARIK
                     AHKGSRFVHAFLVQHWVKFLLLVHVKRGKDSDAWKSSIEAMDQLCWSVEPKNTIEERR
                     KLATVVPGMLKRLTTGLKVAGVDEAASARFFAELMKRHTEVMGKEEKTAAQETAAASS
                     TAKPHAAPASKEPAKPAPAEPGKAPVPTEAKTATRISAPTPKEAAKAAPAAPGKPPAP
                     GVAKATGVKPAPAAKEAAKPAPPTPAKPAAPSPAETGDSESLDFTSTITVRNPFGGGQ
                     VQVDEVNFADLPGASPTQARQAAKDAELTGKLAEGTWVEIREKSQGSDEETRRPARLS
                     YISPMKNSYLFVDRQGKTALECSRAELARRMRLGNVVIMDEAPLFDRIMGGLVGKLGG
                     AAAPH"
     gene            complement(8935..9723)
                     /locus_tag="AC16CX_19365"
     CDS             complement(8935..9723)
                     /locus_tag="AC16CX_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014702.1"
                     /translation="MGRSLKVLTLIAVAFVAAGAAGVVAAQAGLSYDRTGLGLKPQTA
                     APVLPADLTFGGEAVQIWQAGRAPDFALYGGTREVSRVGLYSAESYGGIVYSLSRGWG
                     SSFEAGYAPESVLSPRRYALTGQLHASFSEGRALSVGLKYRLYDADIGLRSDAASDTS
                     LSSVYTLAPMRVPGTSVGPSYQLQFSYQYSAAGSFGLALGRDVETYTPSFDPTANAPR
                     QLTFTGQHWLTPTWALSYDVLASDPANPLRLQSLGLRLGVRYRF"
     gene            10000..10983
                     /gene="ybiB"
                     /locus_tag="AC16CX_19370"
     CDS             10000..10983
                     /gene="ybiB"
                     /locus_tag="AC16CX_19370"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HIR3014703.1"
                     /translation="MSVMQMIKLLAGETAETRELSEREACELMSAMLDGGLGELELGA
                     ALALLEHKPVTLAELLGYCAALAQRCSRLKRPAGSPRPVVFACYHGVRSQPHLLPVVA
                     LALQRLGVPVLVHGALGGAGGVAAAYVFRELGVFPCASLTLAQSRLEERKLAFVPAAV
                     LAPGLAELLALKGRLGFGGFIQSLARLLMPFESEALHVVGTNSVTGSVLLREYLATSG
                     TRALLLEGVEGEAFADPRRRPRLEYVTANECRVLFDAEAVMLKHPATLPEAVEATSVA
                     AWIRRVLAGEAPLPLPLVNQIACCLYGAGYTDDMNQAKAIVAVETGSLAAA"
     gene            11221..11718
                     /locus_tag="AC16CX_19375"
     CDS             11221..11718
                     /locus_tag="AC16CX_19375"
                     /inference="COORDINATES: protein motif:HMM:NF017945.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3014704.1"
                     /translation="MMDFIQLGIGRWLHIASGVMWIGLLYYFNFVQVPALADAGKDGT
                     GAGITKHVAPRALFWFRWAAVATWLLGALLLGKNFFDAFLFLNRAFYAIGVGAWLGTI
                     MIFNVWVLIWPNQQKILGLKPASDEEKARARRVASLASRTNTMLSIPMLFFMAAAGHQ
                     GIFFG"
     gene            complement(11807..12250)
                     /locus_tag="AC16CX_19380"
     CDS             complement(11807..12250)
                     /locus_tag="AC16CX_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR3014705.1"
                     /translation="MRITASAVLLSALLLAGVPACAQLPEIALSVNGHKLTVEVASND
                     ETRMQGLMHRRILPENRGMLFVFTDAAVHAMWMMNTHIPLSVAFLDERGVIINIADMQ
                     PQTRDTHAAAKPAKYALEVNQGWFRKRGVKPGVKVEGIERAPPAR"
     gene            complement(12267..12638)
                     /locus_tag="AC16CX_19385"
     CDS             complement(12267..12638)
                     /locus_tag="AC16CX_19385"
                     /EC_number="4.99.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HIR3014706.1"
                     /translation="MKSALVLFAHGARDALWSEPFRALQRAVSNRRPDLTVELAFLEL
                     MEPALGDCVARLAGSGYTRIIIAPLFLAQGGHLKKDLPRLLKELCTKHPGADITVLPP
                     IGEVTELLDAMAEWLVNSVSR"
     gene            complement(12751..13200)
                     /gene="dut"
                     /locus_tag="AC16CX_19390"
     CDS             complement(12751..13200)
                     /gene="dut"
                     /locus_tag="AC16CX_19390"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3014707.1"
                     /translation="MRTIDVKILDERLRGQLPQYVTAGSAGLDLRACIDAPITLAPGQ
                     AELIPSGIAIHLADPGLAAVVLPRSGLGHKHGIVLGNLVGLIDSDYQGQIFVSTWNRG
                     AASFIVNPMERLAQLVVVPVVQVGFNVVEEFDASRRGGGGFGSTGKG"
     gene            complement(13306..14520)
                     /gene="coaBC"
                     /locus_tag="AC16CX_19395"
     CDS             complement(13306..14520)
                     /gene="coaBC"
                     /locus_tag="AC16CX_19395"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR3014708.1"
                     /translation="MAESSRKSVVLGVTGGIAAYKAAQLTRDLQRHGIEVQVIMTQAA
                     QGLVTPATFQALSGGPVYTDMWDASVPDNMAHIELSRGKDAIIVAPATADFIAKLANG
                     LADDLLSALCLARECPLIVAPAMNVQMWENPATRRNIDRLLSDGVAILGPASGDQACG
                     EIGMGRMLEPDQIAEAVAGFLAPKLLEGARVLITAGPTFEPIDTVRGITNRSSGKMGY
                     AIARAALDAGARVTLISGPVSLAAPAGAEVARVETAAEMFEAVKKHAPRADIYIGVAA
                     VADYRVDKPRRHKIKKTDEEQLKLRLVPNPDILGWVAGRPNPPFCVGFAAESRNLDAY
                     ADEKRRRKKVPLMVANLAQEAIGSDDNEVTLLDDGGTHKLPRAPKEIVARQITAHIAK
                     LYGARKRRKREE"
     gene            14670..15344
                     /gene="radC"
                     /locus_tag="AC16CX_19400"
     CDS             14670..15344
                     /gene="radC"
                     /locus_tag="AC16CX_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR3014709.1"
                     /translation="MAITDWPLDDRPREKLIAKGAEALSDAELVAIFLRTGLRGRSAL
                     DLARELLGRFGSLTALFAADTAALCSTSGLGAAKSAQLQAVMEMARRALREKLDRGSA
                     LGSPQAVRDFLRLKLQDKPHEVFAGVFLDAQNRVLAVEELFRGTLTQTSVYPREVVKR
                     ALHHNAAALIFAHNHPSGIAEPSRSDEALTQALKQALALVDVKVLDHFVIGAGSAMSF
                     AERGLL"
     gene            15457..>15886
                     /locus_tag="AC16CX_19405"
     CDS             15457..>15886
                     /locus_tag="AC16CX_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3014710.1"
                     /translation="MYYKHIMLAVDGSEPSRKAEKECIAFAKSSGAKVTAIHVVSHFH
                     LHFQPWATPKEMHTKIEKEHEEEAMENARKMISELETRAKAGGVKCEGLVVVGDHPYE
                     EIINNAEKRKCDLIMMASHGRRGIDALLLGSETAKVLTHSK"
BASE COUNT         2686 a         5026 c         5258 g         2916 t
ORIGIN      
        1 tttattgcta tgctcgtccc acgcacgcct accgcaatct ctcgccgggc atagattggg
       61 agacgcggct cttcgccaag gtgaacgccg cggagaagct gcgcgaggag ctttcgcgcc
      121 ctggctaccg ctgcgaagtg atctcgctcg gcgccaacac cgatccctat cagccgatcg
      181 agcgcaattt caggattacc cgcggcatcc tcgaggtgtg cgccgaatac aaccagccgg
      241 tcggcatcgt caccaagaac gccatggtcg agcgcgacat cgacatcctt gcgccgatgg
      301 cgcggaagaa cctcgtggcc gttttcatct cgtgcaacaa cctcgaccac gatcttgcgc
      361 gccggctcga gccgcgctgc tccgcgccgg cccggcgtct gcaggcgatg cagaggctgt
      421 ccggggccgg cattccggtg ggcgtgctgg tcgcgccggt gattcccttc ctcaccgatc
      481 accagatcga gccggtgctg gaagctgcgt gggaatgcgg cgcgcggcag gcgggctacg
      541 tcctgatgcg gctgccgtgg gaggtgaagg acttgttcaa ggaatggctc gaacgcaact
      601 acccgctcaa ggcgagacac gtgatgagcc gggtgcacgc gatgcgcggc gggcgcgaca
      661 acgatcccgg tttcggcagc cgcatgacgg ggcagggcga gctcgcccgg ctgctcgccc
      721 agcgcttcag gaaagcctgc gagcggctcg gctacaatac ggaggcgcgc aaccgcgagc
      781 tcgacacctc gcagttccgg ccgccggcgc gaccggggca gatggcgctg ttctgacgaa
      841 tcaggaaaca gtgaccggta gaggagagga aaagtgaaga acgaggtcgc gttatacgtg
      901 ggcgaatctc tcggcaaata cgggtttccc agcgggcacc cgttcggccc cgaccggcag
      961 gacgcgttct ggaaggaggc ggtgaagcag ggcctcgacc agcgcgtcgc gatcaccgac
     1021 ccgcgcatgg cggcggagga ggagctgcac cgtttccact tgcccgagtt cgtggcgtgg
     1081 gtgaaggagc ggtccgagga gggctacggc gccatcgact acggcgacac gccggcttac
     1141 ccgggcgtgt acgaggcggc ggcgacggtg gcgggctccg cgctgcacgg gctcgacacg
     1201 atcatgagcg ggggcgcgct gcgcaccttc cagccgatcg gcggactgca tcacgcgcgg
     1261 cccggcgctg cggcgggctt ctgcgtgttc aacgacgtgg gcgtggtgat cgacacgctg
     1321 cgcgaccagt acggcgtccg gcgcatcgct tacgtggaca tcgacgtgca ccacggcgac
     1381 gggctctact acccctacga ggcggacccc gacctcatct acgccgacat ccaccaggac
     1441 gggcgcacgc tctatcccgg gaccggccac gcgcacgaga ccggcaaggg ggcggcgcaa
     1501 ggcaccaagc tcaacatccc gctgctgccg gggatgggcg accgggagtt cctcaaggtg
     1561 tgggaagccg tggaggcgca cctgcgcgag ttcaagccgg agttcatcct gttccagtgc
     1621 ggcgccgaca gcctcgcggg cgatccgcta gcgatgctgc agtacacgcc ggccgcgcac
     1681 gcgcacgcgg cgcggcgcct ctgcgccctg gcgggcgaaa tgaccggcgg gcgcatcatg
     1741 ggcttcggcg gcggcggcta ccaccgcggc aacctcgcgg cggcgtggtg cgcggtgctg
     1801 gaggagttcc tcaaggccta aaagacggga gagggggaga gcgggagggc gcgagagcga
     1861 caaacccgtg aaagcaggag ggcgtgagcg cgtggggtcg agttagtgac ttacgatctt
     1921 tccctctccc gatttcccga cctcccgatc tccagggttc ttccctctcc cgatttcccg
     1981 acctcccgct ctcacggggt ttttcgatct cccgatttcc cgacctcccg ccctcccgtg
     2041 ttttgtcagg cgaggagacg ctgcaccctc cgagccgccg cgatgtgacc atcgcggctg
     2101 aactgctcgt ggttccggtc cacctcggcg aggtcgtagc gatagcttgc catcaggccg
     2161 taggactggc gcatgccgcg ggcggaggtg tcggcgagcc agaagatgcg gctcacgcgc
     2221 gcgccattgt tgaggtggaa ttgggcgacc gagtcgagtg ggtgcgcgcc cgacttttcc
     2281 tgaaggaggt agcgggcgca gagcttctcc atcggctcgc gcagcgcttc cccgagccgg
     2341 gcgtcggccg gccattcggg gcgcgccaag gcgtccgcga gcgcggtccc ggcgtcggga
     2401 tacccgagcg ctgcagcaag cttcgcggtt tctgctgccg gcagcagtgc ccgaccctcg
     2461 gcgagcgcct ggtcgagcca cttgcggaat ccgggcagcg gcgacagcgt ggcgaatata
     2521 tggagccgcg gcaggtcgag ctggaggtcc tcgattacgc gcttcagcag caagttgccg
     2581 aagctcacgc cgcgcaggcc cttctgggtg ttggagatcg aatagaagac cgcggtattg
     2641 gcggtgcgcg ggtcctgcac cggcgcgctc tggtcgagca gcaactgtac gctgccgggc
     2701 aggcccttcg cgagcgcgat ctcgacgaag atcagcggct cgttcggcag cctgggatgg
     2761 aagaacgcgt agcagcggcg atcggtgtcg aggcggttcc gcaggtcgtt ccacgactca
     2821 atcgggtgca cggcctcgta ggtcatgagc ttctcgagca cgagcgccgg cgactgccag
     2881 ctgatgcgct gcagttccag gaagccgggg tcgaaccagc tctcgagcag cccgtcgagc
     2941 tcgactttca gcacttcgag cgccggctcc tgcggcaggg ccgcgagcag gtcggcgcgc
     3001 aggtcgacga gaaacttcac gccttccggc agcaggttga actgcgcgag gaacttcgcg
     3061 cgcggggcgt cgagcgcgat gcgcagacgc gcctccgccc gggagagctc ggcgtcgttc
     3121 gccgccacct ggatcgccgc gatggcctgt tccagctctt cgcggttcgg cgcaaactcg
     3181 tgcgtgatca ggttgaggat tgcggcgcgc tgagacgcgc cggtcgcgcg gtagatggcc
     3241 gcgatacgct cggcccgctc gcgtgaggag acttcgccgc cgcgctgcat ccggcattct
     3301 tcgagcagcg gcttgatcct ttcgatcagg cgtgcggtcg cccggtcggc cgccgttgcg
     3361 ccggccgcgg aaaagaccgt tgccgcgagc cgctgccacc agtggcgcag ccgctcgagc
     3421 gcggagagcg ggctggggga gcttgctgcc cggttcatcg acatttttcc tggaattcca
     3481 cttatggctt gatactattc cgcccgcgtt tcgatgtaca gaacaatcaa gaaagccttg
     3541 tgctagatca atgcccgagt tgccaaaaac agacagtttg acaggccatg cgcctcggcg
     3601 ttctagatca gtcgcccgcc atcagaggcc acacccccgc gcaggccgtc caggggacca
     3661 tccgcctcgc caaggccgcg gagacgctcg gctaccaccg ctgctggctc gggtgacgag
     3721 cgcaagcagt tcatgagcgc gtgcctgaag ggctgattgc cctccacccg tcgcaggggg
     3781 catactggga cgatcggtat atgtccctgt ttttttgagc ccatcttaaa aaaacaaaag
     3841 tggattcaca ggattgacga gagtggcctt attcatgcgc ggcgtgtgga actgcctggt
     3901 cgcgctggcc ggcgccctgg tcccggccgc gggggggatg atggacgcgc aggcccagga
     3961 tatctacgag ccgcagcgca ggcgcatggt ggaggagatc gcggcgctca cgcgcgagac
     4021 gcgcttcgag accggccgcg cgagcctttc cgagcgggtg atggcggcga tggcgaaagt
     4081 gccgcgccac gagttcgtgc ccgagggcga gcggcgcaat gcctacgcca accggccgct
     4141 gcccatcggc cacggacaga ccatctcgca gccctatatc gtcgcgctga tgaccgatct
     4201 catggaggtc aagcccggcg accggctgct cgagatcggc accggctccg gctaccaggc
     4261 ggcagtgctg gccgagctcg ccgtcacggt ttacaccatc gagatcgtcg aaccgctcgc
     4321 gcgcgaggcg caggaacggc tgagacggct cggttaccgc aacgtggtgg cgaaagcggg
     4381 cgacggctac cggggctggc ccgagcacgc gccgttcgac gccatcatgg tgaccgccgc
     4441 gccgcgcgag gtaccgcagc cgctgatcga ccagttgaaa cccggcgggc ggctggtggt
     4501 gccggtcggc acccagatcg gcggccagtc cctgctcgtg atcgagaaaa aactcgacgg
     4561 gaccgtcacg cgccgcaacg tccttgctgt ccgctttgtg cccctcactg acaaagcggg
     4621 acggcagcag taagaccgaa ccgctcgccg ctgaggacgc ggaggaagtt cgaagagcgt
     4681 caataatggt gacgggccac tccctgtgtt cccgggcgtt gaacggcgcg tcgcgtccat
     4741 tcgaacggcc ggaagtaaac cggcgacgtc agccgtccgg atttactcgg gcttgatgtt
     4801 ctgctcccta accaccttca cccagcggtc gtagtccttg cggatgcgct cgccgaagcg
     4861 ctccggcgtg ccgcccgagg gcaccatgcc ctgcgcctcg aggtttttct tcatgtccgc
     4921 gtcctgcagg aacttgttca ccgtcgcgtt gagcttctcg acgataccgc gcggcgtgtt
     4981 gcggggcgcc atcacgccga accacagctc gacctcgtag ccgggcagcg tctggccgag
     5041 cgtgggaacg tcgggcagcg aataccaacg cctggaggtg gtcaccgcca gcggccgcag
     5101 cttgccgctc cggaagtgcg ggaacgtggg cagcaagctg cccacgatga gctggcactg
     5161 gcccgagagc aggtcgggca acgccgcgcc ggtgctcttg taggcgatgc cgaccatgct
     5221 caccttggcc atgttgatga ggagttccgt ggcgaggtgg gtgagactgc ccactccggg
     5281 cgtgacgtaa ttgagctgcc ccggcctggc ctttgccagc gcgatcagct ctttcgcgtt
     5341 cttcaccggc agggaagggt gcaccgaaag cacgaacggc gtatagccca cttccgcgat
     5401 cgggatgatg ttgttgatcg ggtcgaaggc cggcttgtgc gtcgccgggg tggcggagaa
     5461 gctgcccgac atcaccatga tggtgtagcc gtcgggcggt gacttgacgg tcagctccat
     5521 gccgatcagg ccggaggcgc cgccgcggtt gtcaaccacg aactgctgcc tgaggttttc
     5581 ggtgagcttc tgcgcgacct gccgcgcggt gatgtcgctg cctccgcccg gggcgaacgg
     5641 cacgatgacc cgcaccggct tggccgggta gtcctgtgcc gggataaccg acggcttgaa
     5701 aagcaggata gcggcggcaa acggcgtgat gagaaatcgc cgggataggc tgccgttcat
     5761 gtttttgccc ccttgtagcg acgtgatcgg ccgatgccga tcccatggtg tttatcagcc
     5821 gtagcgtgtt gccgacaagc gcactcgacg agccgcttgt acgcgctcag tgcggcgccg
     5881 cggccccgcc cagcttaccg acgagaccgc ccatgatgcg atcgaacagc ggcgcttcgt
     5941 ccatgataac cacgttcccg aggcgcatgc ggcgtgcaag ctcggcccgc gaacactcga
     6001 gcgcggtctt gccctggcga tcgacgaata ggtagctgtt tttcatcggg ctgatatagg
     6061 acaatctggc cggtcggcgc gtttcctcgt cgctgccctg gctcttctcg cgtatctcca
     6121 cccaggtccc ttcggccagt ttgccggtga gttcggcatc tttcgcggcc tgtctggctt
     6181 gagtcgggct cgccccgggc aggtccgcaa agttcacctc gtcgacctgc acctggccac
     6241 caccaaacgg gttcctgacg gtgatggttg acgtgaaatc cagcgattcg gaatcgccag
     6301 tttcggcagg gcttggtgcg gcgggcttgg ccggtgtcgg cggggccggt ttggccgctt
     6361 ctttggccgc aggcgcaggc tttacgccgg ttgctttggc cacgccagga gcgggtggtt
     6421 tgcccggtgc cgccggcgcc gctttggccg cttccttggg cgtaggcgcg gatattcgag
     6481 tcgcagtctt ggcctcggtc gggacgggcg ccttgccggg ctcagcgggt gccggtttgg
     6541 ctggttcctt ggatgcaggc gcggcatggg gtttcgccgt gctgcttgcc gcggccgtct
     6601 cctgcgccgc agtcttctcc tctttaccca tcacctcggt atggcgtttc atcaattcgg
     6661 caaaaaagcg cgcgctagcc gcttcgtcga cgccggcgac cttcagaccg gtggtgagac
     6721 gcttcaacat tcccggcacc acggtggcca gcttgcggcg ttcctcgatc gtattcttgg
     6781 gttcgacgct ccagcagagc tgatccatgg cctcgatcga gctcttccag gcatcactgt
     6841 ccttgccgcg cttgacgtga accagcaaca ggaacttgac ccagtgttgc acgagaaacg
     6901 cgtgcacgaa gcgcgacccc ttgtgagcct tgatccgggc cttgatctcg tcctgcgccg
     6961 tggccttggc aagggcaagg ctttcctgtt gctcgatccg tttcgctgta gcctccgtct
     7021 cctgttcggc gcgcttatcc tcttcagcga tgagcgcctg gagccgctcg cgcgtcgtgt
     7081 cgaaaatctc caggttgtcc tggaagccgt tcatgagttc ctggatgatg gtctcgagac
     7141 ggccaaacag cgggctggag gagttgaatt cggccggcag gcgcacggca atctcgccca
     7201 gcgtattaag cagctgccgc gcgggatggg ttttctggga gaagaacgcc ttgtccgtga
     7261 tcgcgacctt gagcatcggg atctgcatac ggccgatgag ccccttcacg ctgatcggta
     7321 tcttgggctc gtcgaaaagc tggtcgaaca acatggccac gatgtccagc gtcatggcat
     7381 ccatctggcc catgccggcg ccgacgttgc tgctcttgag ctcgcgcagg acgttcgtcg
     7441 ttcccgcctc gccgccaagg gcgggcaatg ccacggtctg gccatcgctc gctgtcatgg
     7501 cgctgacgtt gccctgctgg atgcgcgtga gcgagttcag caaatctgcg ccctgaagca
     7561 ctacctggac cccgcccgcg ccgggcacgg caacgctccc cgcggcgccg agttgcgccg
     7621 tcgcctgagg gtttttcgcg agcaggcttt gcagtacgga gaagaagtcc tgctcgctac
     7681 cggcagcttt atcggcggcc ttcttttcgg tcccgggcgc ctcatcgtcg gcgtgcgcgc
     7741 cggcggctgg cgccttgccg ccctcctgac gcgatacccc atagcggatt tttggcagga
     7801 tcgaattctt caccagcagg gcgttcacgt ccttatagat ggaccggatg gcgtcgacga
     7861 catggtcgtc gaacaacttg agaagaacca ttctcagctt gatgttcgaa tcaacctgcc
     7921 ggcacgtgta cttgtaggcg tcgcagatcg cctgcgggct gaagggattg tcttccgcct
     7981 gcagatccgc gtcaccgagc agcacgccca cgcgctggtc gagcgcgacc agttcctcat
     8041 cgcagaaccg gcgcagcttg gccgccacgt tgttgaactt gagggtttcg tcgagatcgt
     8101 cctcgcctac caggtcgagc tcgagcgcgg acaggtcgaa ctcggagaaa ctctggccga
     8161 tttttcttac ccggttgctg cgggtctgaa actcccgcag atagcgcgtt cggaaagagt
     8221 cctcaatgga tttgcgctgc gcaagcactt tgtcccgggt ctccctgtag agtacttgcg
     8281 tttcctggtt ctgggtttca ttgatgagcg cggttagcga ttcctccact ttgtcgagca
     8341 tgccggccaa cgcctggtcc agacgctcgc aaacgaggtc gcaactttgc tccaggatct
     8401 tgtcgaatgg agacgcctgt tgcgctgcag cagggggatc cggattgggc atttgaacga
     8461 ctatgaatgt tgatttccac cgttccgtgc acagggcatg cgaacggttc gcgctcgaag
     8521 taccgctcac ccaggaggaa gcacgaagtc tttgtttccc acggaatctg gccccctgtg
     8581 accagaataa ctatttatta tgcctcagct tctgcccgga taatgcgaca tttgccatac
     8641 gcgacaacaa tggcattgga ttgccccggc ctgcatgttt catgactgtc ccgcttttgg
     8701 ggcatgcgat cgagccgcgc agggaaagcg cctgccgcat gcagggggcc tacaaggttt
     8761 ttgggccatt tcgcgagagg cggcgacacc acccccttat gtaactgtaa cgggggttat
     8821 cgctcgggct ggcttgaacg ccgcagcgac gatgcggcac gaggtggaca agccgtgctg
     8881 tctgcggagg gcggaagaaa gttgacgacc gggcgattcc ttgcgcccgc ccggtcagaa
     8941 gcggtagcgc accccgagac gcagccccag gctctggagt cgcagcgggt tggcgggatc
     9001 gctggcaagc acgtcgtagc tcagggccca ggtcggcgtc agccaatgct ggccggtaaa
     9061 ggtcagctgt cgcggcgcgt tcgccgtggg atcaaaagac ggcgtatagg tttccacgtc
     9121 ccggcccagc gcgagcccga atgaaccggc cgcgctgtac tggtagctga actgcagttg
     9181 atagcttggc cccacgcttg tgccgggcac acgcatgggc gccagcgtat agacgctgga
     9241 aagcgacgtg tcgcttgccg cgtcggaccg cagcccgatg tccgcgtcgt atagtcggta
     9301 cttgagcccc acactcaatg ctcttccctc gctgaacgag gcgtgcaact ggccggtgag
     9361 cgcatagcgc cgcggcgaga gcaccgattc cggtgcatag cccgcttcga atgaggaacc
     9421 ccatccgcgc gacagggaat agacaatgcc gccgtaagat tcggctgagt acagaccgac
     9481 tcgggaaacc tctctcgtgc cgccatataa cgcgaaatcg ggcgcacgcc ctgcctgcca
     9541 gatctggact gcttcgccgc cgaaagtcag gtccgccggg agcaccggag cagccgtctg
     9601 cggcttgagt ccgagaccgg tgcggtcata ggagagcccc gcctgggccg caaccacgcc
     9661 ggccgcaccc gcagcaacga acgcgacggc gatgagcgtc aaaactttga gcgaccgacc
     9721 catttgtgag gaggattcgg ttttcagggg ctggcggcaa ttcaagtgtg ccccttccac
     9781 tccggtttgt gcagtcaagt ttatcaattc cgtcggcttg ggttgcccta ctgtggcgct
     9841 ggcgcaacag cctgcaacga tccgcaccac gtttaagcat ggactgcgag ccatgcacca
     9901 agttctggcg ccggtatcag gagtgtttct caagttattg aattaacacc ttaaaaaaat
     9961 ggcacaccag ttgctagctg ctgtaagcgg gatgttgcaa tgagcgtgat gcagatgatc
    10021 aagttgctag ccggtgagac ggccgaaacg cgcgaattga gcgagcgcga agcctgtgag
    10081 ctgatgtcgg caatgctcga cgggggactc ggtgagctcg aactcggcgc ggctctcgcg
    10141 ctgctcgagc acaagcccgt gacgctcgcc gagctgcttg gctattgcgc cgcgctcgcg
    10201 cagcgctgca gccggctgaa acggccggcg ggctccccgc ggcccgtagt attcgcctgc
    10261 tatcacgggg tgcgcagcca gccgcacctg ctgccggtgg tcgcgctcgc tctgcaacgg
    10321 ctcggcgtac cggtgctggt gcacggcgcg ctcggtggcg ccggcggagt cgccgcggct
    10381 tacgtattcc gcgagctcgg cgttttcccc tgcgcgagcc tgacgctggc gcagtcccgg
    10441 ctcgaggagc gcaagctcgc tttcgttccg gccgccgtcc tggcgccggg actggcggaa
    10501 ctgctggcgc tgaaagggcg tctcggtttc ggcggcttca ttcagtcgct cgcccggctg
    10561 ctgatgccgt tcgagagcga ggcgctgcat gtggtcggaa ccaattccgt gacgggaagc
    10621 gtactgctgc gcgaatatct ggcgacgagc ggaacgaggg cgttgctgct cgaaggcgtg
    10681 gaaggggagg cgtttgcgga tccccgacgc aggccgcggc tcgagtacgt gacggcgaat
    10741 gaatgcaggg tactgttcga tgcggaagcc gtcatgctca agcatcccgc gacgttgccg
    10801 gaagccgtcg aagcgacttc ggtggcggcc tggattcgcc gcgtgcttgc gggagaagcg
    10861 ccgttgccgc tgccgctcgt caaccagatc gcatgctgtc tctatggcgc gggctatacc
    10921 gacgacatga accaggccaa ggcgatcgtc gccgtcgaga ccggcagcct cgccgccgcc
    10981 tgagcgcgcc accgcccttg cccgtgcgta atgcgggcgg gtgattcgtt caagcattgc
    11041 tgctggcgtt gaagtgtact agaatacgcg ccagcatcat ctcgtgtctc atttccaggt
    11101 ggaggaataa caatgaaata ttttctcgac tcgcttgggt ggacggtttt ctggggcgtc
    11161 gttctcacgc tgatccttta caacatcgtg aagttcgcgt tgcaatagaa ggaggcggcc
    11221 atgatggact tcatacagct cggaatcggg cgctggctgc acatcgcctc cggcgtcatg
    11281 tggatcggcc tgctctacta cttcaatttc gtgcaggttc cggcgctggc ggatgccggc
    11341 aaggacggca ccggcgcggg catcaccaag cacgtcgcgc cgcgtgcatt gttctggttc
    11401 cgctgggccg ccgtggcgac ctggctcctc ggggcgctgc tgctcggcaa gaactttttc
    11461 gacgcgttcc tgttcctgaa ccgcgccttt tacgcaatcg gcgtgggcgc atggcttggc
    11521 accatcatga tcttcaacgt gtgggtgctg atctggccca accagcagaa gatactgggg
    11581 ctgaagccgg cgagcgatga ggagaaggcc agagcgcgcc gcgtcgcgtc cctcgcctcg
    11641 cgcaccaaca ccatgctctc catcccgatg ctgttcttca tggcggcggc ggggcatcag
    11701 ggcatattct tcggctgatc cggacgcagt ccgaaaaaac gcccgagcgg acctcgggcg
    11761 ttttttattc caagttcatg gtccgaacct ggaacctgaa accgcgtcag cgcgctggcg
    11821 gggcgcgctc gatgccctcg accttcacgc ccggcttgac gccacgcttg cggaaccagc
    11881 cctgattgac ctccagggcg tacttggcgg gcttcgcggc ggcgtgcgtg tcgcgcgtct
    11941 gcggctgcat gtcggcgatg ttgatgatca cgccgcgctc gtcgagaaac gcgacggaaa
    12001 gcggaatgtg ggtattcatc atccacatgg cgtgcacggc ggcatccgtg aacacgaaca
    12061 gcatgccgcg gttctcgggg aggatcctgc ggtgcatgag gccctgcatc cgcgtctcgt
    12121 cgttgctcgc gacttccacc gtgagcttgt ggccgttgac cgagagcgcg atctccggca
    12181 gctgcgcgca cgccggcacg ccggcgagca gcagcgcgga cagcagaacg gcagaagcgg
    12241 tgatgcgcat cagctcgttt aacgcctcag cgcgagacgc tgttgaccag ccattcagcc
    12301 atcgcgtcga gcagctcggt gacttcgccg atcggcggca agacggtgat gtccgctccc
    12361 gggtgtttcg tgcacagctc cttcagcaga cgcggcaggt ccttcttgag gtggccgccc
    12421 tgggccagga acagcggcgc aatgatgatg cgcgtatagc cgctccccgc caggcgcgcc
    12481 acgcagtcac ccagcgccgg ttccatcagc tcgaggaatg cgagctccac ggtgagatcg
    12541 ggccggcggt tcgaaaccgc ccgctggagc gcgcgaaacg gctcgctcca tagcgcatcg
    12601 cgcgcaccgt gcgcgaacag cacgagggcg gatttcatgc gccgaaggat gaaggatgaa
    12661 ggatgaggga tgacccccgc agtgcgggaa tcaagaccaa gttatctttc gggcggagcc
    12721 ctctgctggt catccttcct ccttccgcct tcatcctttg cctgtgctgc cgaacccgcc
    12781 gccgccgcgg cggctcgcgt cgaattcctc caccacgttg aagccaacct gcaccaccgg
    12841 cacgaccacg agctgggcga ggcgctccat ggggttgacg atgaacgacg cggccccgcg
    12901 gttccaggtg gaaacgaaaa tctggccctg gtagtcagag tcgatcagcc cgacgaggtt
    12961 gcccagcact atgccgtgct tgtggcccag tcccgagcgc ggcaggacca ccgcggccag
    13021 ccccgggtcc gcgaggtgga tggcaatgcc cgagggaatc agctcggcct ggccgggagc
    13081 gagcgtgatg ggcgcgtcga tgcaggcgcg cagatcgagc cccgccgatc ccgccgttac
    13141 gtattgcggg agttggccgc gcagccgctc gtccaaaatc ttcacgtcta tcgtgcgcat
    13201 ctggatttga ccgctagcgc tattgcgcgc gccagtaggt gaggagtaag gggtgaggag
    13261 taaggtgaaa agcgccaaaa gttgtacctc ctcacacctg actccttact cctcccgctt
    13321 tctacgcttg cgggcaccgt aaagtttcgc gatgtgggcg gtgatctgcc gcgcgacgat
    13381 ttccttcggc gcacgcggga gcttgtgagt gcccccgtca tcgagcaggg tgacctcgtt
    13441 gtcatcggag ccgatcgcct cctgcgcgag gttggcgacc atcagcggca ctttcttccg
    13501 gcgccgcttc tcgtcggcgt aggcatcgag gttgcggctc tcggcggcga agccgacgca
    13561 gaacggcgga ttgggacgcc ccgccaccca gccgaggatg tcgggattcg gcacgagcct
    13621 gagcttgagc tgttcctcgt cggtcttctt gatcttgtgc cggcgcggct tgtccacgcg
    13681 gtagtccgcc accgcggcga ccccgatgta gatatcggcg cggggagcgt gcttcttcac
    13741 cgcctcgaac atctctgccg ccgtctcgac gcgcgccact tcggcgcccg ccggggcggc
    13801 gaggctcacg gggcccgata tcagggtgac cctggcgccg gcgtccagtg ccgcgcgcgc
    13861 gatcgcgtag cccatcttgc ccgagctgcg gttggtgatg ccgcgcacgg tgtcgatcgg
    13921 ctcgaaggtc gggccggcgg tgatcagcac gcgcgcgccc tcaagcagct tgggagcgag
    13981 gaagccggca accgcttcgg cgatctggtc gggctcgagc atgcggccca tgccgatttc
    14041 gccgcacgcc tggtcgccgc tcgcgggccc gaggatcgcc acgccgtcgc tgaggagacg
    14101 atcgatgttg cggcgcgtgg cggggttttc ccacatctgg acattcatgg cgggcgcgac
    14161 gatcagcggg cattcgcgcg cgaggcacag cgccgagagc aggtcatccg ccagcccgtt
    14221 cgccagcttg gcgatgaagt cggcggtggc gggcgcgact atgatggcgt ccttgccgcg
    14281 cgaaagctcg atgtgcgcca tgttgtccgg gacgctcgca tcccacatgt cggtatacac
    14341 cggcccgccg gacagcgcct ggaaggtcgc cggcgtgacc aagccttgcg ccgcctgggt
    14401 catgatcacc tgaacctcga tgccgtggcg ctgcaggtcg cgggtcagct gcgccgcctt
    14461 gtacgccgcg atgccgcctg tgacaccgag cacgaccgat tttcgagaac tttcagccat
    14521 gtagcgcttc aatttcaagt gagggcaaga gtgtaaacca gcgatcagca ctcagcaatc
    14581 agctgtcagc tatcagccgt tgcgaggtaa acgaccgcca atgtggaagc gttgaaggaa
    14641 acaagctgat tgctgatcgc tgaatgctga tggctattac tgattggccc ctcgacgacc
    14701 gccctcgcga gaagctgatc gcgaagggcg cggaagcgct gtccgacgcc gagctggtgg
    14761 cgatttttct gcgcaccggg ctgcgcggca gaagcgcgct cgaccttgcc cgggagctgc
    14821 tggggcgctt cggctcgctg accgcgctgt tcgcggccga taccgccgcc ctgtgctcga
    14881 cgtcgggact gggcgcggcg aagtcggccc agctccaggc ggtgatggaa atggcgcgtc
    14941 gcgcgcttcg cgagaagctc gaccggggct ccgcgctggg ctcgccccag gcggtgcgcg
    15001 attttctccg gctcaagctt caggacaagc cgcacgaggt gttcgccggc gttttcctcg
    15061 acgcgcagaa ccgggtgctg gcggtggagg agctgttccg cggcaccctc acccagacca
    15121 gcgtctatcc gcgcgaagtg gtgaagcggg cgctccacca caatgcggcc gcgctgatct
    15181 tcgcccacaa tcatccgtcc ggcatcgcgg agccgagccg ttccgacgag gcgctcaccc
    15241 aggccctgaa gcaggccctt gcgctggtgg atgtcaaggt gctcgaccac ttcgtcatcg
    15301 gcgccggctc ggcgatgtcg ttcgccgagc gcggcttgct gtaggaaact gccgatgaac
    15361 gccgataaac cggaacaaat ggacaggatt ggcttaagat aaaggctgtg ccgcgcgggc
    15421 ggcgtcatcc tccgatcaca tcaaggggag cctcctatgt attacaagca catcatgttg
    15481 gccgttgacg gttccgagcc gtcgcgcaag gcggaaaagg agtgcatcgc tttcgccaag
    15541 tccagtgggg caaaagtgac cgcgatccat gtggtgtcgc actttcacct gcatttccag
    15601 ccatgggcaa cgcccaagga gatgcacacc aagatcgaaa aagagcacga agaggaagcc
    15661 atggagaatg cccggaagat gatctccgaa ctcgagactc gcgccaaagc ggggggcgtc
    15721 aagtgcgaag gtctggtcgt cgtcggggac cacccttacg aggagatcat caacaacgcc
    15781 gagaagcgca aatgcgatct gatcatgatg gcctcgcacg ggcgcagggg tatcgatgcc
    15841 ttgctgctcg gcagcgaaac cgccaaagtg ctgactcact cgaaaa
//
LOCUS       DBNGWZ010000620         7002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3000938, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000620 DBNGWZ010000000
VERSION     DBNGWZ010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3000938"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="AC16CX_19410"
     CDS             complement(<1..1096)
                     /locus_tag="AC16CX_19410"
                     /inference="COORDINATES: protein motif:HMM:NF024821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014711.1"
                     /translation="MPPISTSPSEKLQQAFGCLNAHDLAGTERLCREILGETPDHPDA
                     LHLLGVVRLIGGDPREAVSLIGRALQTRPHDAAVLENLGVAHLAASESAQAEALFRRA
                     LAQGASHASLHMRLGLALGAQGKSAEAVSALRRAAGQLPGDPDVHLNLGNALAEHGQP
                     EEALACYQRVLALQPGHAVARFNLGNLFRRMGRFEEAEASFRQALLVTPDDPDFHNNL
                     GLLHEQQQRLEDALSCYRQALALNSGHQHARNNLGNVLRTQGRLEEAVACFEKVLSDA
                     PDHVDASINLGVARMEQGRYPEAQALYEKVLRLEPRNFEGHYNLGRLLRLQGRLAEAI
                     VHYRHALDADPKRAMAHSELGAAHRQAGDLD"
     gene            complement(1110..2933)
                     /locus_tag="AC16CX_19415"
     CDS             complement(1110..2933)
                     /locus_tag="AC16CX_19415"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014712.1"
                     /translation="MIVPTLQQAADNFGAGNYRQAEQLCEQVLECAPDHPEALHLLGM
                     LRLSSGRAVEAIRPLQGALRADPRNLSALEALSIAFVTVDEHRKAEEVIRQMLAAGAD
                     LPVVSARLGITLAHQGKWNEALQAFEEAVRGDPQFPEAHYQLGNTLLELGRPGEALVR
                     LERALALEPRHAGAHNALGAALQALGRTEEAIAAFRRALALDEKFAPAQQNLGNVLFE
                     QGRLEEARTCLEHAIQLTNDHARTRCLLGAVLQEQGRWAEAAEHHERALALDPGFAGA
                     QYNLALARLFLQQFDRAWPLYERRLDSEGIRERLRKDPVTVGRFERLPRWRGPGERDG
                     SEVAVWAEQGIGDQMVYSMLIPELLEAGARFVYEVDARLLAAYQRAFPGSRFVSLREP
                     PDEALPQAGRVLLAGSLPGLFRRTRESFAHQPRRVLSALPERVAYYRGRLDLLGPGLK
                     VALSWYSSRREKLLAPRKSVPLIQYAPLLAVPGAQFVDVQYGDTSAERKAVEAATGVR
                     LLRFEGVDYFNDLEEVLAILEACDLLITISNATAHFAGALGKRTWLLYLADNPPFHYW
                     VHDISYRSLWYPAVEIISAPHFAEWAPLMEHVAVKLAQNLQ"
     gene            complement(2959..4437)
                     /gene="mgtE"
                     /locus_tag="AC16CX_19420"
     CDS             complement(2959..4437)
                     /gene="mgtE"
                     /locus_tag="AC16CX_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR3014713.1"
                     /translation="MLESAGIAITIPLPMAERDEIRPSVHDSLRRVTELLQKHELAEG
                     DAHRQQGPRQELVENLVHKLHVVELQKLLDSQHPADVAYMLESLPLEQRLVVWDLVKA
                     EREGEILLEVSDAVRETLIAHMDEQELVAAAEQLDTDEIADLAPDLPSEVIQDVFKSL
                     SPEEREQLRAAMSYPEGTVGALMDFDMISIRDDITVEVVLRYLRRLDEMPEQTDQLFV
                     VDHAGRLKGLLPVNRLLVTDPETQVATVMQPEFVKFHPQEKAQDASGAFERYDLVSAP
                     VVDDDGRLIGRVTVNAVMDYIREETDSDMLTAGGLREDEDLFASVWNSVRNRWTWLAL
                     NLVTAFIASRVIGLFEHSIAQLVALAALMPIVAGISGNSGNQTITFIVRALALGQITA
                     ANARKLFAKEIGVSALNGLIWGSVVGLFAFLIYHNWQLGVVMTGAMVLSLVLAAVMGV
                     AIPLTMQKLGRDPALGSSVMITAITDSGGFFIFLGLATVFLV"
     gene            4560..4961
                     /locus_tag="AC16CX_19425"
     CDS             4560..4961
                     /locus_tag="AC16CX_19425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR3014714.1"
                     /translation="MTPIKPHHRLDAWKQAMALVRTIYEATRFFPVEETYGLTSQLRR
                     AAVSIPSNLAEGAGRDGHREFLKFLSIARGSLSELETQLLIAADLGYLSPEHRAFALL
                     ERVSRLITGLHRKLALRPQSAMRQFPRSASS"
     gene            complement(5022..6869)
                     /locus_tag="AC16CX_19430"
     CDS             complement(5022..6869)
                     /locus_tag="AC16CX_19430"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014715.1"
                     /translation="MSGTLDHKLEKAAAGAANDTLSSDDGLERVRALRLDGKLAEAVD
                     ACHRALERAPGDARAWNELAHALRWQGRLDEARAAAERALEQEPRLAEAWFNRGAVQV
                     AQGEAARGIESYRKALELRPAFAEAWSNLGDALGTTGDKPGEIEAYRRAIEIDPQLAP
                     VWSNLGSALLETGRIGEALLACRRATELDPDFAAGWNNLGNALRECGEHEEAVRACES
                     ALRLAPGLAEAWSSLGAASHSLGRYEDAIRAHRNAIDIDPGNAHHHFNLGMTLQHCGR
                     SAEAIASLRHALALDPGHAQAHWDLSFALLGSGQLPEGWEEYEWRWRRPNAEAKRYDF
                     TSWDGDASKSRRLLLWGEQGVGDEIMYASMVPELASSSLSVTLETDARLAPLFNRSFP
                     GVTVIPRRDPPTAGPASHDCQVPLGSLGRWLRRSFESFPRHRGYLKADRLRAESFRKQ
                     LLGDQATRLVGISWKSANKEFGHLKSNELRDWQGIFRVPHVHFVDLQYGDTAGERTRL
                     EQQPGAHLEHLPDLDLYNDLEGLAALCAACDLVITVSNVTAHVAGALGRAAWLLVPKA
                     NGRLWYWFSGRTDSPWYPLMRIFTQQTAGSWREVLDEVARELAVFVKNP"
BASE COUNT         1232 a         2348 c         2167 g         1255 t
ORIGIN      
        1 aatcgagatc gccggcctgc cggtgggcgg cccccagctc gctgtgggcc atcgcccgct
       61 tcgggtcggc atcgagcgcg tgccgatagt gaacgatcgc ctccgccagc cgtccttgca
      121 gcctcagaag tcggccgagg ttgtagtgcc cctcgaagtt gcgcggctcc aggcgcagaa
      181 ccttctcgta cagcgcctgc gcttcgggat agcgcccctg ctccattcgt gcaacgccga
      241 gattgatgga ggcgtcgacg tgatccggcg catcggacag cactttctcg aagcaggcaa
      301 ccgcttcttc aagccgccct tgcgtcctca gcacattgcc caggttgttt cgggcatgct
      361 gatgaccgga gttgagcgcc agggcctgtc gatagcacga taacgcgtcc tcaagccgct
      421 gttgctgctc gtgcagcagc cccagattgt tatggaagtc gggatcgtcc ggcgtgacga
      481 gcaacgcttg cctgaaggat gcttccgcct cctcgaaccg tcccatgcgc ctgaacaggt
      541 tgcccagatt aaagcgcgcc accgcatggc ccggctgcaa tgccagcact ctttggtaac
      601 aggccagcgc ctcctccggc tggccgtgct cggcgagcgc gttgcccagg ttgagatgaa
      661 cgtccggatc tccgggcaac tgcccggcgg cccggcgcag cgcgctcact gcttccgcag
      721 atttgccctg tgcgccgagc gcgagaccca gtcgcatgtg gagcgaagca tgcgatgcac
      781 cttgcgcgag cgcgcgccgg aacaatgctt cagcttgggc cgattccgaa gccgcgagat
      841 gcgctacgcc caggttctcg agcacggcag cgtcatgcgg cctggtctga agggcccggc
      901 cgatgagaga caccgcctcg cgcgggtcgc ccccgatgag acgcacgacg ccgagcagat
      961 gaagtgcgtc gggatgatca ggcgtctccc cgagtatctc cctgcagagc cgctccgtgc
     1021 cggccagatc atgggcgttg aggcagccaa aggcctgttg cagcttttcg cttggcgagg
     1081 ttgagatggg gggcatccgg cgtaacgtcc tattgcaaat tctgtgcgag cttgactgca
     1141 acgtgctcca tcaacggcgc ccattcggcg aaatgcggcg cggaaatgat ttcgaccgca
     1201 ggataccaga ggctgcgata gctgatgtcg tggacccagt aatggaatgg cggattgtcc
     1261 gccagataca gcagccaagt gcgcttgccc agcgccccgg caaagtgcgc tgtcgcatta
     1321 cttatggtaa tgagcagatc gcacgcctcg aggatcgcca gcacttcttc cagatcattg
     1381 aagtaatcga caccttcaaa gcgtagcaga cgaaccccgg ttgccgcctc cactgccttg
     1441 cgctcagcag atgtatctcc atactgcacg tccacgaact gcgccccggg aacagcgagc
     1501 agcggtgcgt attggatcaa tggcacactc ttgcgcggcg ccagcaattt ttcgcgcctg
     1561 ctgctgtacc aggaaagcgc aaccttcagt cccggaccca gcaagtcgag ccggccacgg
     1621 tagtacgcca cccgctccgg cagcgccgag agcacgcggc gcggttgatg cgcaaagctc
     1681 tcccgcgtgc gccggaacag cccaggcagg gaacccgcta acagcacgcg cccggcctgc
     1741 ggcagcgctt cgtccggcgg ctcgcgcagg ctcacgaacc ggctgccggg aaatgcccgc
     1801 tggtaggccg caagcaaacg ggcatccacc tcgtacacga agcgcgcgcc agcctccaga
     1861 agctccggaa tcaacatcga gtacaccatc tggtcgccta tgccctgctc ggcccaaacg
     1921 gcgacttcgc ttccgtcccg ctcgcccggc ccccgccagc gcggtagccg ctcaaaccgg
     1981 cccaccgtga ccggatcctt cctcaggcgt tcgcggatcc cctcgctatc caaccgccgc
     2041 tcatacagcg gccacgcccg atcaaactgc tgaaggaaca gccgcgccaa ggcaagattg
     2101 tattgcgcgc ccgcgaaccc cgggtcgagt gccagtgccc gctcgtggtg ctcggccgcc
     2161 tcggcccacc tgccctgctc ctgaagcact gctcccagaa ggcagcgcgt acgggcatga
     2221 tcgttcgtga gttgtatggc gtgctccagg catgtcctcg cctcctccag ccggccctgt
     2281 tcgaacagca cattacccag attttgctgc gcgggcgcga atttctcatc cagggccagc
     2341 gctcgccgaa acgcagcaat cgcctcctct gtccggccga gcgcttgcag cgccgcgccc
     2401 agcgcgttgt gagcaccggc atggcgcggc tcgagggcca gcgcgcgctc aagacgtacc
     2461 aacgcttctc ccggccgccc cagttcgagc agtgtgtttc caagctggta atgcgcctct
     2521 gggaactgcg ggtcaccgcg cactgcctcc tcaaaggcct gcagggcctc gttccattta
     2581 ccctgatgcg cgagcgttat cccgagtctc gcgctgacga ccggcaggtc ggcacccgcg
     2641 gcgagcatct gccggatcac ctcttccgcc ttgcggtgtt cgtcgacggt gacgaatgcg
     2701 atcgataacg cctcgagcgc gctgagattg cgcggatcgg ctcgcagcgc gccttgcaag
     2761 ggacggatcg cctcgacggc gcgtccgcta ctgagcctga gcattccaag caaatgcagc
     2821 gcttcggggt ggtctggagc acattccagc acctgttcgc acagttgctc cgcctggcga
     2881 tagttgccgg cgccaaaatt gtcggctgcc tgctgcagcg tcggcacgat cattgaaggg
     2941 ggaatcatga agacggcgct agaccaggaa aacagtcgcc aagcccagga aaatgaaaaa
     3001 cccgccgctg tcggtgatgg cggtgatcat cacgctcgag ccgagcgccg ggtcgcgccc
     3061 cagcttctgc atggtgagcg gtatcgccac gcccatgacg gctgcaagca ccaaagagag
     3121 caccatggca ccggtcatca ccacgccgag ctgccagttg tggtagatca ggaaagcgaa
     3181 caggcccacc acgctgcccc agatgaggcc gttcaacgcg ctcaccccga tttccttggc
     3241 gaagagcttc ctcgcgttcg ccgcggtgat ctggcccagc gcgagcgcgc gcacgatgaa
     3301 cgtgatggtc tggttgcccg agttgccgct gatgcccgcg acgatgggca tcagggcggc
     3361 gagcgcgacc agctgggcga tcgagtgctc gaacagcccg atcacgcgcg acgcgatgaa
     3421 cgcggtgacc aggttcaagg cgagccacgt ccagcggttc ctgacgctgt tccacaccga
     3481 cgcgaacagg tcctcgtcct cgcgcaagcc ccccgccgtg agcatgtcgc tgtcggtttc
     3541 ctcgcggatg tagtccatca cggcgttcac cgtgacgcgc ccgatcagcc ggccgtcgtc
     3601 gtccaccacc ggtgcggaga ccaggtcata gcgctcgaag gcgccggacg cgtcctgcgc
     3661 cttttcctgg ggatggaact tgacgaattc cggctgcatc accgtcgcga cctgcgtttc
     3721 gggatccgtg acgagcagcc ggttgaccgg cagcaggccc ttcagccgcc cggcgtgatc
     3781 gaccacgaac agctggtcgg tttgttccgg catctcatcg agccgccgca ggtagcgcag
     3841 gacgacctcg accgtgatgt cgtcgcggat gctgatcatg tcgaaatcca tcagcgcgcc
     3901 gaccgtgccc tcggggtagg acatcgccgc gcgcagttgc tcgcgctcct cgggagagag
     3961 cgacttgaag acgtcctgga tcacctcgct cggcaggtcg ggcgcgaggt cggcgatctc
     4021 gtcggtatcg agctgctcgg cggccgccac cagctcctgc tcgtccatgt gcgcgatcag
     4081 ggtttcgcgc accgcgtccg agacttcgag caggatctcg ccttcgcgct cggccttgac
     4141 caggtcccag acgacgaggc gctgctcgag cggcagcgac tccagcatat aggcgacgtc
     4201 cgccgggtgc tgcgagtcga gcagtttctg cagctcgacg acgtgcagct tgtgcacgag
     4261 gttttcgacc agctcctggc gcgggccctg ctggcgatgg gcatcgcctt ccgccagttc
     4321 gtgcttttgc aggagctcgg tcactcgccg caagctgtcg tgcacgctcg ggcggatttc
     4381 gtcgcgttcg gccatgggta gcgggatcgt gatcgcaata ccggcgctct ccagcatcgc
     4441 cggtgcgaca ttctaactga cacgtcagcg gtgagcagta agccgtaagc ggtaagcggt
     4501 aaaccggatg cgattttcga gcgttaaagg gggttaacga ccacaaccgt cgaatttgca
     4561 tgactcctat caaaccgcac cacaggctgg atgcctggaa gcaggcaatg gcgttagttc
     4621 gtactattta tgaggctacc cgttttttcc cggtggagga gacctatggg cttacctctc
     4681 agttaagacg tgccgcagtt tcaataccga gcaacttggc agaaggtgcc ggcagagacg
     4741 gccacaggga gtttctgaag ttcctgagta ttgctcgtgg atcattaagt gaattagaga
     4801 cgcaattact gatcgcggct gatttggggt acctttcccc cgagcatcgg gctttcgccc
     4861 tgctagagag ggtctcgcgc ctcattactg gattgcacag aaagctggcg cttcgacctc
     4921 agagcgctat gcgtcaattt ccgcgctcgg ctagcagctg accgcttacc gctcacggct
     4981 gaccgcttac cgcgcaccgc tcaccgctta ccgctcacgg cttacggatt cttcacgaac
     5041 acggcgagct cacgcgcgac ttcgtccagc acctcccgcc agcttccggc ggtctgctgg
     5101 gtgaaaatgc gcatcaatgg gtaccacgga ctgtcggtcc gcccggagaa ccagtaccaa
     5161 agcctgccat tggccttcgg cacgagcaac cacgccgcgc ggcccagcgc gcccgctaca
     5221 tgggcggtca cgttgctcac ggtgatcacc agatcgcagg ctgcgcatag cgcggcgagc
     5281 ccttccaagt cgttatacag gtcgaggtcc ggcagatgtt cgaggtgagc gccgggctgt
     5341 tgttcgagcc gggtgcgctc gccggcagtg tcgccatatt gaagatccac gaagtggacg
     5401 tgcggcaccc ggaatatgcc ctgccagtca cgcaattcat tgctcttgag gtggccaaac
     5461 tccttgttcg cgcttttcca cgagatgccg acgaggcgag tcgcctgatc gccgagcaat
     5521 tgcttccgga acgattccgc gcgcaaccga tcggccttga gatagccccg atgccgcggg
     5581 aagctttcga atgagcgcct gagccaccgc cccaggcttc ccaacggcac ctggcagtcg
     5641 tggctggcgg ggcccgccgt gggaggatcg cgccgcggga tgacggtgac gccgggaaag
     5701 gatctgttga aaagcggcgc cagccgtgca tcggtctcca aggtcacgct gagcgaagag
     5761 gaggccagct ccggcaccat gctcgcatac atgatctcat cgccgacgcc ctgctcgccc
     5821 cacaacagca gccggcggga tttggaggcg tccccatccc atgacgtgaa gtcgtagcgt
     5881 ttcgcctctg cattggggcg gcgccagcgc cactcgtact cttcccaccc ctccggcaac
     5941 tggcccgaac cgaggagcgc aaagctcagg tcccagtgcg cttgcgcgtg gcccggatcg
     6001 agcgcgagcg catggcgaag ggatgcgatc gcctccgcgc tgcggccgca atgctgcagc
     6061 gtcatgccga gattgaaatg atggtgcgcg tttccggggt cgatgtcgat ggcgttgcgg
     6121 tgagcgcgga tggcgtcctc gtagcggcca agcgaatgcg acgcggcgcc gaggctgctc
     6181 caggcctcgg cgagccccgg agcgagccgc agcgccgact cgcacgccct gaccgcctct
     6241 tcatgctcgc cgcattcgcg cagcgcgttt cccaggttgt tccagcccgc ggcaaagtcc
     6301 ggatcgagct cggtcgcgcg ccggcaagcg agaagcgcct ctccgatccg gcccgtttcc
     6361 agcagcgcgc tgccgaggtt gctccacacg ggagcaagct gcggatcgat ctcgatggcg
     6421 cggcggtaag cctcgatttc cccgggcttg tcgccggttg tgcccagcgc gtcgcccaga
     6481 ttgctccagg cttcggcaaa tgcgggcctg agttcgagcg ccttgcggta ggactcgatg
     6541 ccccgggccg cttcgccttg tgcgacctgt acggcgccgc ggttgaacca tgcctcggca
     6601 agccgcggtt cctgctcgag cgcgcgttcg gccgccgcgc gcgcctcgtc cagccggccc
     6661 tgccagcgca acgcatgcgc cagctcattc cacgcccgcg cgtcgccggg cgcacgctcc
     6721 agcgcgcgat ggcaggcatc aacggcctcg gcgagcttgc catcgagccg cagcgccctc
     6781 acccgctcga gcccgtcatc ggatgacaac gtatcgttcg ccgcgccggc agcggctttt
     6841 tccagcttgt gatccagcgt tcccgacatg tcaaaaaacg gtaagcggta agcggtgagc
     6901 agtaattaga acccgcttgc tctcaagttc caagcgcagc cttcggcggg cctgactccg
     6961 ggaacaacgt atccgcgtcg acagtcgcca gcttgactag ag
//
LOCUS       DBNGWZ010000621         6503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_102764, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000621 DBNGWZ010000000
VERSION     DBNGWZ010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_102764"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..1884
                     /locus_tag="AC16CX_19435"
     CDS             571..1884
                     /locus_tag="AC16CX_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014716.1"
                     /translation="MVAARFTPDYQFEALSTGKGDAAAKGAGRGALSCGEFLRGSGGT
                     GIGAVAVGLLFLVCLPVGATVGAIYGASHAASAEQVKETKAVAQRGIAALKLQDEAVD
                     AALRYGRNVGLEIGRLPQTMGPATPDEVPSYAETKGTVDSVIEISVLHANAFTSGGRE
                     LRVSLGLQARVRVLSARDGKVIDILNMKYISEARAIDEWLAADGQAIKTEFDRGSASI
                     AEQAIDEIMLIYHPKTVPQQVESRETERVPSYALRAIEPRIRNKFYLSTARITYGHLE
                     RYPLSELQPTFRWEPWPRGFDIVPGNGPRQARQVRYDLRIFGATGILYEPRGLVEPVH
                     WLDATGIIIYERRGLVEAVHRLEQPLEPCRTYRWTVRARFVLNGAPRATEWTGAYDTM
                     SGQVAPWWWRRGSGVPALAFVPGSVIPLYPIVETPNVDGTTCPGR"
     gene            complement(2118..3077)
                     /locus_tag="AC16CX_19440"
     CDS             complement(2118..3077)
                     /locus_tag="AC16CX_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014717.1"
                     /translation="MKVLILAVALFVAGTAHAQQYPSKPIRLIVPFAPAGPNDLLGRM
                     VGQKITEQLGQAVVIENRGGAGGTIGLDAAAKSPGDGYTLSMGGSSNMAVAPSLYAKL
                     PYDSLRDFTPVINVAHVPYGVGLNPTVPAKNVKELVAIAKRKANYLSYGSSGTGSMSS
                     LAAELFKSMSGTQIVHVPYKGTAPALTDVVSGQIDMMFADLSLIQNLARAGKLRLVAV
                     TGSKRSPAAHDVPTISESGLKGYVIEPWFGVVGPAGVPRDIVTRLNGVIAAALKAPDV
                     TQRLDALGYEPIGGTPEQFAATIKADIARYAKIVKTAGIKAEP"
     gene            complement(3185..4159)
                     /locus_tag="AC16CX_19445"
     CDS             complement(3185..4159)
                     /locus_tag="AC16CX_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014718.1"
                     /translation="MNSLSRTFFVGALLAALPVAALSQGAYPTKPVRLIVAFPPGGST
                     DIIARLVGQKLSERLGQQVIIDNRGGAGGTIGTEIAARATADGYTLTMGTTSTHVIAP
                     AAYPNVKYDPVKDFTPITLVASTPYLLVLHPSVQAKTLKDFVALMKSQPGKLNYASAG
                     TGSTTHIAMEMLKIASGTDIVHVPYNGNGPAGTALMGGQVQALFGSMPAVLPFARAGK
                     MRPIAVGTSKRSPALPDVPTVSESGYPGFEVSLWLGFFAPKGAPAPIVSRLYDELVKI
                     AKSPEMKEQFERNGADPLTTTPAELDKLMKAELAKYSKVIKAANIQIK"
     gene            complement(4268..5245)
                     /locus_tag="AC16CX_19450"
     CDS             complement(4268..5245)
                     /locus_tag="AC16CX_19450"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014719.1"
                     /translation="MKIARSASVLLAGLMFAAQGWAQDAAKDYPSRPVRWVVPFTPGA
                     SNDIIARLVAQKLTDATGQQFVVDNRGGAGGLVGAETVAKALPDGYTLLLANPGPNVN
                     SVLLHKKPPYKISEFDPVVWIGYAPLIILANPKFPPNNAKELLDYARANPGKVSWGSS
                     GVGSSLHIGLAVFQVATGVNVVHVPYKGAAPALTDVVGGQINVMHTTSVSGEAHIKAG
                     RVKVLGIAAKKRQAVLPNVATLEEQGIKGAEAIVWFGMAAPAKTPRPIIAKLNREVNK
                     ALGMPDVRQRLDQLGLEIEGGTSEKFDAFIKSEAARLTKLIKARAVQVE"
     gene            complement(5302..6279)
                     /locus_tag="AC16CX_19455"
     CDS             complement(5302..6279)
                     /locus_tag="AC16CX_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HIR3014720.1"
                     /translation="MKISILDDYQNVVRTLKCFPRLAGHDVTVWNDHTKDTDTLAQRL
                     KDAEVLVTIRERTPIRAPLIERLPKLKLISQTSVYPHIDVEACTRHGIVLCSDLHPGE
                     PSYATAELAWCLIISSLRSVPQEVAALKSGRWQSTIGYALRGRTLGILGYGRIGAVMA
                     RYARAFEMGVLAWGRERSAERARADGVEFAASKGDLFERSDVLTLHVRLTPETRGLVT
                     ASDLVRMKPTALIVNTSRSGLIEPGALVAALKSGRPGMAAVDVYEEEPMLDPQHPLLK
                     MDNVVCTPHLGYVERDGYERIFSAVFDQILAFIAGKPFNVINPEALQKR"
BASE COUNT         1140 a         2068 c         2057 g         1238 t
ORIGIN      
        1 gccgatggcc gcttacgacc tattctgttg aaaaactctt tttccaccga cgatgaaaaa
       61 attctagccg ttataggaag cgaggcgcgc ttcaggctag ggggatacat gaaagagttg
      121 atgtcgcgat gtaagacctc ctgagcggcc tatagagtgg agccaggtca aattttcgat
      181 tgcctcaata tcaggccgaa atttgccgca ctcgagattt cgagtttttc aacggaatag
      241 acccaaagcg gtcattcccg cgggccgtgt ataagccgaa ctattatggg attgcccacg
      301 gctgagcatc ggcgtagcgt tttcggtagc ggcccatcga gacgaacgcc aggatcggat
      361 gggcaggaag aagtttagac ttaaatcggc ggcgagacgc cgagctcgta aacctcgctg
      421 ccagctgtga ggggggaaca catgacgaga atgcgccgat tggtgacgtg ctcgcttgcg
      481 gtggtggtat gcatgcagac gtcctgcgca gtcccggtaa ggccagagca cctcgcggag
      541 cccgacagga cgtccctcgg gcacgttgca gtggtggcag cacgcttcac tccggactac
      601 cagttcgagg cgctctcgac cggcaagggt gacgcggccg ccaagggcgc tggcagaggc
      661 gcgctgtcct gcggtgaatt cttaaggggc agtggtggga cgggtatcgg ggccgtcgct
      721 gtgggacttt tattccttgt ttgcctgccg gtgggtgcca cagtgggagc gatctacggc
      781 gcttcccacg ccgcgtctgc tgagcaggtc aaagagacaa aggcagtcgc acaacgcggg
      841 atcgcggcgc tcaaactaca ggacgaggcg gtcgacgcgg cgttgcgtta cggtcggaac
      901 gtcgggctgg agatcggtcg cctaccgcaa accatgggcc cggcgacgcc cgacgaggtg
      961 ccttcttacg cggagacaaa gggcacagtg gactcagtca tcgaaataag cgttctccac
     1021 gcgaatgcct tcacgagtgg aggccgggaa ctgagggtct cgctaggcct gcaggctcgg
     1081 gttcgggtcc ttagcgcgcg ggacggcaag gtgatcgaca tcttgaacat gaaatacatc
     1141 agcgaggcgc gtgctataga cgaatggcta gcggctgacg gtcaggcgat caagaccgag
     1201 ttcgatcgtg gttccgcctc gattgccgag caggctatcg acgaaatcat gctgatctac
     1261 caccccaaga ctgtacccca gcaggtggaa tctcgcgaaa cggagcgagt gccctcgtac
     1321 gcactccgcg cgatcgagcc ccggatccgc aacaagttct acctcagcac ggcgaggatt
     1381 acctatggtc acctcgagcg atatccgctc agcgagcttc agccgacttt ccgctgggaa
     1441 ccgtggcctc gaggcttcga cattgttccg ggaaacgggc cgaggcaagc gcgtcaggtt
     1501 cgctacgatc tgcgcatatt cggtgcaacg ggaatcctct atgagccccg cgggttagtt
     1561 gagccagtgc attggcttga cgcaacggga atcatcatct atgagcgccg cgggttagtt
     1621 gaggcagtgc atcggcttga gcagcctctg gagccttgcc gtacctacag gtggaccgtg
     1681 cgtgcgcggt tcgtgctcaa cggtgcaccg cgcgcgacgg agtggactgg ggcatatgac
     1741 accatgagcg ggcaggttgc tccttggtgg tggcggcgtg gcagcggcgt cccggctctt
     1801 gctttcgtcc caggcagcgt gatcccgttg tatcccatcg tcgaaactcc caatgtggac
     1861 ggtacgacgt gtccgggccg gtaggtgacc gtgcgagcgc tgatctccaa cgctcacggt
     1921 cactgaatga cccctcttgg ccgtttgcag ccattcctga aagtgcggtt aaagtgcggc
     1981 tgccaccgaa caacggcttg caggacgacg cgccgcgagc ggcgcgcgcc tgaagccgga
     2041 cgttagggca cttcgataac agcttggaac cgctcgcccg ccagtcacgc tttcctcacg
     2101 tcacgcatta tcaggaatca aggctctgct ttgattcctg ccgtcttcac gattttcgcg
     2161 tagcgcgcga tgtccgcctt gatggtagcg gcgaattgct ccggcgtgcc gccgatcggc
     2221 tcgtagccga gcgcatcgag ccgctgcgtc acgtcgggcg ccttcagcgc cgcggcgatg
     2281 accccattca ggcgcgtgac gatgtcgcgc ggcacgccgg caggcccgac cacgccgaac
     2341 cacggctcaa tcacatagcc cttcaacccg gactccgaga tcgtgggaac gtcgtgcgcc
     2401 gcgggcgaac gcttcgagcc ggtcacggca acgagtctca acttgccggc cctggcgagg
     2461 ttctggatca gcgagaggtc ggcgaacatc atgtcgatct ggccgctcac gacgtcggtc
     2521 agcgccggcg cggtgccctt gtagggaacg tgcacgatct gggtgccgga catggacttg
     2581 aacagctcgg cggcgaggct cgacatgcta ccggtgccgg atgagccgta gctcaggtaa
     2641 ttcgctttcc gcttcgcgat cgcgaccagc tccttcacgt tcttcgccgg cacggtgggg
     2701 ttgaggccaa ccccgtacgg cacgtgggcg acgttgatga ccggcgtgaa atccctgagc
     2761 gagtcgtacg gcagcttggc atagagactc ggtgccaccg ccatgttgct cgagccgccc
     2821 atcgagaggg tgtagccgtc gccgggggac ttggcggcgg cgtcgagccc gatggtgccc
     2881 cccgcgccgc cgcggttctc gatcaccacc gcctgtccca gctgctcggt gatcttctgt
     2941 cccaccatcc ggcccaacaa atcattcgga ccagcgggcg caaacgggac aatcagacga
     3001 atcggcttcg acggatactg ctgcgcatgc gcggttcccg ccacgaataa tgcaaccgcg
     3061 agtatcaaca ctttcatatt catcgccccc ggagtgcccg attatagccc ccgagtacaa
     3121 agcaaacggc acgccagacg cagcaaatgc ggcgcctgcg cgtgccgtgg agagccgttg
     3181 gttactattt gatctggatg ttggcagcct tgatgacctt ggagtatttg gcgagttccg
     3241 ccttcatcag cttgtcgagc tccgccggcg tggtcgtaag ggggtcggcg ccgttgcgct
     3301 cgaactgctc cttcatctcc ggtgacttcg cgatcttcac cagctcgtcg taaagcctgc
     3361 tcacgatggg tgcaggcgcc cccttcggcg cgaaaaagcc gagccacagc gacacctcga
     3421 agcccggata gcccgattcg gagaccgtcg gcacgtcggg cagcgccggc gagcgcttgg
     3481 acgtgcccac cgcgatcggg cgcatcttcc ccgccctcgc aaacggcagc acggccggca
     3541 tgctgccgaa caacgcctgc acctgcccgc ccatcagcgc tgtgccggcg gggccgttgc
     3601 cgttgtaggg aacgtggacg atgtcggtgc ccgaggcgat cttgagcatc tccatcgcga
     3661 tatgagtggt gctgccggtg cccgcggaag cgtagttcag cttgcccggc tgtgatttca
     3721 tgagcgcgac gaaatccttc agcgtcttcg cctgcacgct cggatgcagc accagcaaat
     3781 aaggtgtgga cgccaccaat gtgatcggcg tgaagtcctt caccggatcg tacttgacgt
     3841 tgggataggc cgccggggcg ataacgtgcg tgctggtggt gcccatcgtc agcgtgtagc
     3901 catcggcagt ggcccgcgca gcgatctcgg tgccgatcgt gccgccggcg ccaccgcgat
     3961 tgtcgatgat cacctgctgc cccaggcgct cggagagctt ctgcccgacc aggcgcgcaa
     4021 tgatgtcggt cgagccgccc ggcgggaatg ccacgatcag gcggaccggc ttggtgggat
     4081 aggcgccctg gctcagggcc gccacaggga gcgcggcgag caacgcgccc acgaaaaaag
     4141 tgcgtgacag gctgttcatt tttttcctcc tcttgggtgg tgaacggcgc cgcgcctgcg
     4201 cggcgtcgcg agtgcaggag gattctacta gacccgtcgc gcccggtcta agcgggccgg
     4261 accgccctta ctcgacctgc accgccctgg ccttgatcag cttcgtcagg cgggccgctt
     4321 ccgacttgat gaaggcgtcg aacttctcgg acgtgccgcc ctcgatttcg aggccgagtt
     4381 gatcgaggcg ttgccgcacg tcgggcatcc ccagcgcctt gttcacctcg cgattgagct
     4441 tggcgatgat gggccgcggg gtcttcgccg gcgcggccat gccgaaccac acgatcgcct
     4501 cggcgccctt gatgccctgc tcttccagcg ttgccacgtt gggcaatacc gcttgccgtt
     4561 tcttcgcagc gatgcccagc accttgacgc gccccgcctt gatgtgcgcc tcgccggaga
     4621 cgctggtcgt gtgcatcacg ttgatctggc cgccgacgac gtcggtcaac gccggtgccg
     4681 cacccttgta cggcacgtgc acgacgttga cgccggtcgc gacctggaac accgcgagcc
     4741 cgatgtgcag gctgctgccc acgccggacg aaccccagct caccttgccg ggattcgccc
     4801 tggcgtaatc cagcagctct ttcgcattgt tgggagggaa cttcgggttg gcgagaatga
     4861 tgagcggcgc gtagccgatc cacacgaccg ggtcgaattc gctgatcttg tagggcggct
     4921 tcttgtgcag cagcacgctg ttgacgttgg gcccgggatt ggcgagcagc agcgtgtagc
     4981 cgtccgggag cgctttcgcc accgtttcgg cgccgaccag gccgcccgcc ccgccgcggt
     5041 tgtccaccac gaactgctgg cccgtcgcgt cggtcagctt ctgtgcgacg agccgcgcga
     5101 tgatgtcatt ggaggcgcct ggcgtgaacg gcaccaccca ccgcaccggc cggctcgggt
     5161 aatccttcgc cgcgtcctgc gcccagccct gcgccgcgaa catcaggccg gcgagcagca
     5221 cactcgcact tcttgcaatc ttcatcatgc tctcctctta tcggtatcac ttatcactta
     5281 tcacttatca cctatcactt atcaccgctt ctgaagcgcc tcgggattga tcacgttgaa
     5341 cggcttgccg gcgatgaacg cgagtatctg gtcgaagacg gcgctgaaaa tgcgctcgta
     5401 accgtcacgc tcgacgtagc cgaggtgcgg cgtgcacacg acgttgtcca tcttcagcag
     5461 cgggtgctgc gggtcgagca tcggctcctc ctcatacacg tccactgccg ccattcccgg
     5521 acgccctgat ttcaaggcgg cgacgagcgc gcccggctcg atcagtcccg agcggctcgt
     5581 attgacgatt agcgccgtcg gcttcatgcg cacgaggtcc gacgcggtca ccaggccgcg
     5641 cgtctcgggc gtgagccgca cgtgcagcgt gagcacgtcc gaccgttcga acaggtctcc
     5701 cttggatgcg gcgaattcga caccgtccgc ccgcgcccgc tccgccgagc gctcgcgacc
     5761 ccaggcgagc acgcccatct cgaaagccct ggcgtagcgc gccatcaccg cgccgatccg
     5821 cccgtagccg agtatgccga gggtacggcc gcgcagcgcg tagccgatcg tggactgcca
     5881 gcgcccgctc ttcagcgccg cgacctcctg cgggacgctg cgcagcgagg aaatgatcag
     5941 gcaccaggca agctcagcgg tggcgtacga cggctcgccc gggtgcaggt ccgagcacag
     6001 gacgatgcca tgccgcgtgc aggcttcgac gtcgatgtgg ggataaacgc tggtctggct
     6061 gatgagcttg agcttcggca ggcgctcgat gagcggcgcg cggatcggag tgcgctcccg
     6121 gatcgtcacc agcacttcgg cgtccttcag ccgctgcgcg agcgtatcgg tgtccttggt
     6181 gtggtcgttc cagaccgtga cgtcgtggcc ggcaagcctg gggaagcact tcagcgtgcg
     6241 cacgacattc tggtagtcgt cgaggatcga gattttcatg ttaagtgcgt gaatcgtgat
     6301 ttaaaccttg aaccgtgaaa cttgaacctg ggttttcccc tcactcctca cgcctcactc
     6361 ctcacggctt caatcgagcc atccgctcgc ccgcgtctcg gccagcaccc tgtccacgat
     6421 ctgcggcgcg aggccgacgt aagtcgtcgg atcgagcgcg gcccgtagcg catcagcgga
     6481 caatgcctcc ttcactttcc tgt
//
LOCUS       DBNGWZ010000622         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2873695, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000622 DBNGWZ010000000
VERSION     DBNGWZ010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2873695"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="AC16CX_19460"
     CDS             <1..965
                     /locus_tag="AC16CX_19460"
                     /inference="COORDINATES: protein motif:HMM:NF014542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3014721.1"
                     /translation="VSAVRTGCGKSQTTRWLSLWLRGRRLAVAVIRHPMPYGNLERQA
                     VQRFATRADLDAAQCTLEEREEYEPHVAAGNVVFAGIDYARIIERAQLEARLILWDGG
                     NNDFPFIRPDLHIVLVDPLRPGHETTHHPGEAVLRMADIVVIAKTDAASASDVQRVAD
                     SARRINPRAPLVYAASPVRLDDPLAVRGRRVLVVEDGPTTTHGGMPYGAGYVAATRAQ
                     AAAIVDPRPYATAEILAVYRDYPHIGPVLPATGYTPEQLDGLRRTIDAAPVDLVIAAT
                     PIDLAAALAVNKPIVRARYEFEEVAGAGLSTHVDRFLRRAGLPA"
     gene            1078..>1794
                     /locus_tag="AC16CX_19465"
     CDS             1078..>1794
                     /locus_tag="AC16CX_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014722.1"
                     /translation="MASAFVRALAQFAAPVALLAAAFAIVFYSAALPPSLSGLKVYGP
                     HIVFALGAALAMAFNRGRALLALIVLAGAYGAQQAWLQQGIETLTAQAVYAGLTVFVP
                     LNLAAFAVLPERGTFSRQGALRLGVIGLQAVLVAWTATAGEVGIVEWAYQRFLDPAPV
                     SAGRIPQLGIALISLGLLITMAAALVTRSATPAGLTGAIVAFAIAAHVPTASLTFSVF
                     TAMAELMVTIAVLQDTFRMAF"
BASE COUNT          237 a          661 c          605 g          291 t
ORIGIN      
        1 cggtctcggc ggtgcgcacc ggctgcggca agtcccagac cacccgctgg ctgtcgctgt
       61 ggctgcgcgg ccggcggctc gccgtcgcgg tcatccgcca cccgatgccg tacgggaacc
      121 tcgagcgtca ggcggtgcag cgattcgcca cgcgcgccga tctcgacgcc gcgcagtgca
      181 cgctcgaaga gcgggaagag tacgaacctc acgtcgcggc ggggaacgtg gtgttcgcgg
      241 gcatcgacta cgcgcgcatc atcgagcgcg cgcaattgga ggcgcgcctg atcctgtggg
      301 acggcggcaa caacgatttc ccgttcatcc ggcccgacct gcacatcgtg ctggtggacc
      361 cgctgcgccc ggggcacgag accacgcacc atccgggcga ggcggtgctg cgcatggcgg
      421 atatcgtggt catcgccaag accgacgcgg cgtccgcctc cgacgtgcaa cgcgtcgcgg
      481 attcggcgcg ccgcatcaac ccgcgcgccc ccctggtgta cgccgcctcg ccggttcgcc
      541 tcgacgaccc tctcgccgtg cgcgggcgcc gcgtgctggt ggtggaggac gggcccacta
      601 cgacgcatgg cgggatgccc tacggcgccg gctatgtcgc ggccacgcgc gcgcaggccg
      661 cggcgatcgt cgatccacgg ccctatgcca cggcggaaat cctcgccgtg taccgggact
      721 atccacacat cgggccggtg ctgccggcga ccggctacac cccggagcag ctcgacgggc
      781 tgcggcgcac catcgatgcc gcacccgtgg atctcgtgat cgcggccaca cccatcgatc
      841 tcgccgcggc gctcgcagtc aacaagccga tcgtgcgcgc acgctacgaa ttcgaggaag
      901 ttgccggcgc cggcctgtca acccacgtgg accgcttcct gcggcgcgcc ggattgcccg
      961 cttgacgccc gcatcctcga aacgcgcggc gggaaccgga ccccggccgg cgctctggct
     1021 ataattccat gatgctcggg taccgcaccg ttcccgcccc gccgggccgc gccgcccttg
     1081 gctagcgcgt tcgtccgggc gctcgcccaa ttcgccgccc ccgtcgcgct gctcgcggcg
     1141 gctttcgcca tcgtcttcta cagcgcagcg ctgccgcctt ccctctccgg gctcaaggtt
     1201 tacggcccgc acatcgtgtt tgcgctgggc gccgcgctgg cgatggcatt caaccgcggc
     1261 cgcgcgctgc tggccttgat cgtgctggca ggcgcgtatg gcgcacagca ggcctggctg
     1321 caacagggga tcgagacgct cacggcacag gcggtgtacg ccgggctcac ggtattcgtg
     1381 ccgctgaatc tcgcggcttt cgcggttctg ccggagcgcg gcacgttcag ccgccagggc
     1441 gcgctccggc tgggagtaat cggattgcag gctgttctcg tcgcctggac cgcaacggcg
     1501 ggcgaggtcg ggattgtcga atgggcttac cagcggtttc tggatcccgc gccggtcagc
     1561 gccggacgca tcccgcagct cggcatcgcg ctgatcagcc tgggcctgtt gatcacgatg
     1621 gcggcggcgc ttgtgacgcg ctccgccacc cccgcggggc tgacgggcgc gatcgtcgcc
     1681 ttcgcgatcg cagcgcacgt tcccacggct tccctcacct tctcggtctt caccgccatg
     1741 gcggagctga tggtgacgat cgccgtgctg caggacacct tccgcatggc gttc
//
LOCUS       DBNGWZ010000623         3210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2495131, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000623 DBNGWZ010000000
VERSION     DBNGWZ010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2495131"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="AC16CX_19470"
     CDS             <1..413
                     /locus_tag="AC16CX_19470"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3014723.1"
                     /translation="MPSESVMGSCLCGSVNFEIKPPLSAFRYCHCTRCRKASGSAHAA
                     NLFVPQGQFAWSGGEDRVNHFKLPGARTFAVSFCNRCGSRVPHRIEERQEYLIPAGLL
                     DDDPVARPQNIIFWGSRAPWYVEPQEIPKFTERP"
     gene            502..897
                     /locus_tag="AC16CX_19475"
     CDS             502..897
                     /locus_tag="AC16CX_19475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3014724.1"
                     /translation="MSWEWFFNNGQLRLEPWFLPLLVWGYLQYWLIGKYRIRHGGGGP
                     GMDTPPERLVTTGPFAYCRNPMYLGHIIFLLGLTLSLRSELAALITVATAVWFQFRVL
                     RDEKRLSERFGQPYLDYTARVKRWIPGLF"
     gene            1026..1913
                     /locus_tag="AC16CX_19480"
     CDS             1026..1913
                     /locus_tag="AC16CX_19480"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR3014725.1"
                     /translation="MDITHHALIGGAGMLALGAQGQELAGLGFLAGSVFPDLDVVFMA
                     AGKRFYLKRHQGPTHSLPLAPVYAAALAIVPALQIGFDWRLYLGLLAGLCIHVLLDLS
                     NTFGIQLMWPITRRRFCFDAVFFIDAIAWGLTLGFYALVYLDVAAPGKAALGYAALFA
                     AYVCAKLLLQRRVKKRLGVDIAIPSALNPFGFFLFTRHDGRLETATYNALTNRTSNVR
                     TFTEAAPDVIELARSSEIFRDMQSILRALHVTRVEKERAETVMVAQDMAVRNFGGKFG
                     RTELRFDSGGQLVHEMANI"
     gene            1894..2286
                     /locus_tag="AC16CX_19485"
     CDS             1894..2286
                     /locus_tag="AC16CX_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014726.1"
                     /translation="MRWLTFEIQREPGFRLNLTDLILISSLVIASIIWHGVFPAQHLY
                     LLPLYVGGSFFLFCNVFRIGNRLEAPWYLTFVAITLYGFSRPEFPWLPLLIVCEALKW
                     ALIGYRIRRGPYVGVLYRQLGRFSVVQV"
     gene            2394..3017
                     /locus_tag="AC16CX_19490"
     CDS             2394..3017
                     /locus_tag="AC16CX_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HIR3014727.1"
                     /translation="MSDSVTNLLPEIALAVALAWGAGLRLYAVLFLVGLAGHLGLVVL
                     PGNLAILASPIVIAASGFMTLAEFLADKVPWIDSVWDAVHTFIRIPAGAALAAAVFGD
                     TGPGVVVAAAILGGTVAAGSHLTKAGARSAVNTFPEPFTNWGLSFAEDVLVPVGLWLA
                     LVHPILFFVLISVFAVGAFVLARWLASGLSRLIKQIRQMRSKPVAPE"
BASE COUNT          534 a         1018 c          969 g          689 t
ORIGIN      
        1 tgatgccttc ggaatccgta atggggagtt gcctgtgtgg ttcggtcaat ttcgaaatca
       61 aacctcccct gtcagccttc cggtactgtc attgcacgcg ctgcaggaag gcctcaggca
      121 gcgcgcacgc cgccaatctt ttcgtgccac aagggcagtt cgcgtggtcg ggtggcgagg
      181 atcgggtcaa tcacttcaag ctgccaggcg ccaggacctt tgccgtgtcg ttttgcaacc
      241 gctgcgggtc gcgggtaccg caccggatcg aggagagaca ggaatatctg attcccgcgg
      301 ggctgctgga cgacgacccg gtcgcgcgtc cgcagaacat cattttctgg ggctccaggg
      361 cgccctggta cgtcgagccc caggaaatcc cgaagttcac cgagcgcccc tgaccggcca
      421 attgcgcgct tcatgcatta catccgaaaa cgcctgcgaa gcacgtcgtt tcgaacttat
      481 cttctctacc ctctcatcgt cgtgagctgg gaatggtttt tcaacaacgg gcaactgcgt
      541 ctcgaaccct ggtttctgcc gctgctggta tggggctacc tgcaatactg gctgatcggt
      601 aaataccgga tccgccacgg cggcggcgga ccgggcatgg acacgccgcc cgagcgcctg
      661 gtgaccaccg ggccgttcgc ctattgccgc aacccgatgt atctcgggca catcattttc
      721 ctgctcggcc tgacactcag cctgcggtct gaactcgcgg cactgatcac ggtcgcgacc
      781 gcagtctggt tccaattccg cgtcttgagg gatgaaaagc gcctgagcga gcgtttcggc
      841 cagccttatc tggactatac cgcgcgcgtc aagcgctgga tcccggggct gttttgacgg
      901 taaatcggcc gctggaaggg ggcttgaatg tacaaaacgg gcacgggctc ctcatgaagc
      961 ctcgtgcgcg ctatccttgc ttcctcgcgc gggtgaacgc gagacgctga agggggagcg
     1021 cactaatgga catcacccat cacgcactca taggcggcgc cgggatgctc gccctgggcg
     1081 cccagggaca ggaactcgcc gggcttggat tcctcgccgg cagcgtgttc cccgacctcg
     1141 acgtcgtatt catggcggcc ggcaagcgtt tctacctgaa gcgtcaccag ggaccgacgc
     1201 actcgctccc gctcgcgccc gtctatgccg ccgcgcttgc gatcgtgccc gcgctgcaga
     1261 tcgggttcga ctggcggctg tacctcggcc tgctcgccgg gctctgcatc catgtcctgc
     1321 tcgacctctc taataccttc ggcatccagc tgatgtggcc aatcacccgc cgccggttct
     1381 gcttcgacgc ggtgtttttc atcgacgcga tcgcctgggg gttgacgctc ggtttttatg
     1441 cgcttgtcta tctcgatgtc gccgcgccgg gcaaggcggc gctcggctac gccgcgctgt
     1501 ttgccgccta tgtgtgcgcg aagctcctgc tgcagcggcg cgtcaaaaaa cgtctcggcg
     1561 tcgatatcgc gattccatcg gcgctcaacc cgttcggatt cttcctgttc acccggcacg
     1621 acgggcggct cgagaccgcg acctacaatg cgctgacaaa ccgcacctcg aacgtcagga
     1681 catttacgga agccgctccc gacgtcatcg agctcgcccg ctcgagcgag atattccgcg
     1741 acatgcagtc catcctgcgc gccctgcatg ttacgcgcgt cgagaaggag cgcgcggaga
     1801 cagtgatggt ggcgcaggac atggccgtgc gcaatttcgg cggcaaattc gggcgcaccg
     1861 agttgcgctt tgattcgggc gggcagctgg tccatgagat ggctaacatt tgagatccag
     1921 cgcgagccgg gcttccgtct caacctgacc gatctcatcc tcatctcgtc gctcgtcatc
     1981 gccagcatca tctggcacgg cgtgtttccc gcccagcatc tctatctgct gccgttgtac
     2041 gtgggagggt cgtttttcct gttctgcaac gtcttccgca tcggcaaccg cctggaagcg
     2101 ccgtggtatc tgaccttcgt cgcgatcacc ctatatgggt tcagccggcc cgaatttccg
     2161 tggctgccgc tgctgatcgt ctgtgaagcg ctcaagtggg cgctgatcgg ctaccgcatc
     2221 cgccgcgggc cgtacgtcgg cgtgctctac cggcaactcg ggcggttcag cgtcgtgcag
     2281 gtctgattgc gtattgccag tgacgaaatg acgcgggtaa tcccagtgac aggcgacgtt
     2341 tcacaagacg atgaatcaag tatcgtggtc tcgatccgat caaacctgtt gagatgagcg
     2401 attccgtcac gaacctgctt cccgaaatcg cgctggcggt tgcgctcgcc tggggcgcag
     2461 gtctgcgctt gtacgccgtc ctgttcctgg tgggcctcgc ggggcacctc ggcctggtcg
     2521 tgcttcccgg gaatctggcg atcctggcaa gcccgatcgt gatcgcggca agcggtttca
     2581 tgacgctggc cgaatttctc gccgacaagg tgccgtggat cgacagcgtg tgggatgcgg
     2641 ttcacacctt cattcgaatt ccggcgggcg cggctctcgc ggcggcggtg ttcggagaca
     2701 ccggcccggg ggtggtagtg gccgccgcga tcctgggagg cacggtggcc gcgggatcgc
     2761 atctcacgaa agcgggcgcg cgcagcgccg tcaacacttt tcccgaacca ttcaccaact
     2821 ggggattgtc ctttgccgag gacgttctgg tgccggtggg tttatggctc gccctcgttc
     2881 atccgatctt gttcttcgtc ctgatctcag tatttgcggt cggcgcattt gttctggcgc
     2941 gatggctcgc aagcggcctg tcaaggctga tcaagcagat aaggcagatg aggtcaaaac
     3001 cagtggcgcc agagtaactt ttctggggcc tggaaaccgt accccgttca ccacagtgaa
     3061 ccaaacgaag agtcggtcac attcccagta tccgcccgat ttccgctatg tagcgaccgg
     3121 ccttcaggcc ctcggccacg gctgtgtcca atgcgttctc gaaaccctcc cggatactgt
     3181 gcaagccatc ccttacccag tggtgcctgc
//
LOCUS       DBNGWZ010000624        19835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_855876, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000624 DBNGWZ010000000
VERSION     DBNGWZ010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 19835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19835
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_855876"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16CX_19495"
     CDS             <1..477
                     /locus_tag="AC16CX_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014728.1"
                     /translation="GPTWKGIDGTGYLMSFSDGAIYHGVISWALPEVWPGAVYSLEIG
                     AVGTEGVLTIDDTHRDIVLATNLIQAEGYAPDKRRHVDFLTSYPVGDMALGELRGPMR
                     EETNSWLNRVSLGVTTQHATAREGHNRLMLTKAIDLSARLKRAVKLPIGPEDMGAH"
     gene            531..1514
                     /locus_tag="AC16CX_19500"
     CDS             531..1514
                     /locus_tag="AC16CX_19500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014729.1"
                     /translation="MKTERKYSVLLAAVLTVGAFGAQAAEKFPQRPVRFIIPYAPGGA
                     TDITARQLQTRLSESWGQPIVIDNRAGASGNIALELASRSAPDGYTLFVGNVSTNAIN
                     ETTFASLKVKPSRDLTGVTNLIQLPHLWVVNPGVPASTLKELVQYVKKSGVRLNYGSA
                     GVGAYPHLDAVKFLKIAGIEMTHVPYKGGAGQMIPAIMGNEVQFMFINMASSISNIRA
                     GRIKPLAITTAERRPELPNVPTTAESGFPGVGTNAWNGLFAPAGIPKPLLGRIHADVV
                     KVMETPEMKASLAKVFMAVVVDKSPADFQQFVLKEIKEWGKIVIENNIKVE"
     gene            1549..3231
                     /locus_tag="AC16CX_19505"
     CDS             1549..3231
                     /locus_tag="AC16CX_19505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR3014730.1"
                     /translation="MNKAYQPRHDLVVEKDVEVPVRDGARLKADIFRPTSAGRFPVIM
                     NLGGYRKDKVWVPPPDLEEKANPYMNWETANPLWWVPRGYIAIRVDTRGSGKSPGLFD
                     PWSRQEALDFHDCIEWAAKQKWCNGRVGLSGISYFAMNQWLVAGLRPPALKAIIPWEG
                     AADMYRDLAYHGGIFCLGFIGNWYNNHMAHHLLGKPQQHNPDAFSNNWLWQYMRHSLD
                     TGWFDQRRADWGRIEVPMLTAGNWSGMGLHLRGNTEGFMRAASRHKKLRIHAGTHYHP
                     FYSEDGRRDQLRFFDYWLKGEKNGVMDEPPVKLLIRKGGEGNYEWRSEREWQLKRTRW
                     MRFYLSPSRVIVRNREPAEGLLVTAPPRKKSVITYSAAAMSKAGVASASWTSTALPGS
                     LPRLGVSFETDVLKEDLEVTGPVVLALWLSSSTEDMDLFATIRNIDREGMEVWEVGQQ
                     QQPVPVAKGWLRASHRHLDRSLTRPYRPYHAHDRRLWLKKNEPVKVDVEIWPTCMVFR
                     KGHRIRLDIQPRDGVGSAPYTHYCADYNVGDNTIYTGGTMASHLLLPVIPQK"
     gene            3263..4252
                     /locus_tag="AC16CX_19510"
     CDS             3263..4252
                     /locus_tag="AC16CX_19510"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014731.1"
                     /translation="MRPLSFLLSALFAACLLASAQAFAQKAKDDYPSRPVRMIVPFAP
                     GGASDFVGRIIQPAMADLLKQQVVVDNRGGAAGNIGVEVAVRATPDGYTFLLGNVGTM
                     AINPVYYTKFPYRPLKDLVPITQVVDVPGCLVVHPSLPAKNVKELIAHLKANPGKLNY
                     GAPAPSSANALEMHMFLGATGTNAVQVAYKGGAGPAMIGLLGNEVQMMFVTFSSAVNF
                     AKQGRVRMLGVISPERNPALPEIPTMREQGLQMVVGSWQGLFVPKGTPLPVVNKLYKV
                     GIEMMKDPQVIKRLSDGGVTIVTSKSPADFVAFVKKETERFGKVIKDAKIPTE"
     gene            4529..5800
                     /locus_tag="AC16CX_19515"
     CDS             4529..5800
                     /locus_tag="AC16CX_19515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3014732.1"
                     /translation="MANVGMVGLDWQQRINWDRLRKYRTERARERMKAHGLSAMLCMY
                     DENVRYITGTLTPGWNRLKPGLRYCLLCGDDAPVLFEQGDIGAQVNRHSPWLPKDHVR
                     WSYAWIKGAAGPASTQQVKKFTNGIKQEMKKANVANGKLGVDFIDINMIQIFKDEKID
                     WTDGMTAMMEARAVKNEDEQEALRIVGAIGDAAHWEFMKFLKPGRTENQMTARIMEFL
                     YSIPGMEDVEDVIVSSGPNTWPNWRNFSDRIIQPGDIVFMDLAALTWNGYKSCYYRTY
                     CAGKEPTKEQKDVYATALKWLYDSMKAVKVGTTTREIASKWPSAKEAWGYAEEDQAAA
                     NLWGHGLGLAQYDQPVISRIWSLDHPVEIKEGMVFALETQHGKQFEWGVRIEEMLIVH
                     KDRVELISNFPVEQITVVDPMPGYSTLGWSR"
     gene            5843..6874
                     /locus_tag="AC16CX_19520"
     CDS             5843..6874
                     /locus_tag="AC16CX_19520"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014733.1"
                     /translation="MNMRIWIAVAAAALAAVLSGGCATAPQPPVAMEAVIGNNFGHLP
                     MFVGVEKGLFKKHGIDLKLKVVNTGTDMVNAMTRHEVQIGDMSVTTFLKARHAGSPFL
                     VVGMIMNDATTTFADSPLAIVARRGSGITRVEDLKGKRIGLAKEQTSDEYLKMVLARR
                     GMQYGEMNIQNIMAPPALVSAFADGKLDAMVSWEPFNTMGLMRTPESYEVLRGGGHLS
                     YMMIATVHDPMVQENPKLIQAFVNGLAAASHYTRQNRAEAVQIFAKWVPNVNLEVARK
                     AIQHISYDPRISRQSLRAFEDAQNELLKLTIKGAKPLRITDIVLTSYTEAAERAYPEY
                     FADLPALPR"
     gene            6906..8231
                     /locus_tag="AC16CX_19525"
     CDS             6906..8231
                     /locus_tag="AC16CX_19525"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3014734.1"
                     /translation="MTETTRMALAGISEMLIIPLSAPEATDAARVGPKAANLAALGRA
                     GLPIADGFCVTADAYRMQIAALRLERTTREVFSAEDAQARRSALQLRMRLMEQPIVAE
                     ATEPLLGAWRTITRHGALPGVVRSSALVEDRFGSSFAGQFESFLGLDNETDFLTAVRA
                     CWAALWSPRALRYMATHDVEPANTAMALLIQPLVAAHAAGGGLSQTAEGGMLINATPG
                     LGCAIAQGEVAPDRYALERNGALRESRAGMQYHQASCVHQPRLPRPPAAKKLCLTPSR
                     AEELGRFLLKAEAVMGMPVEIEWALDDTGFKLLQARPLHLDDKPSSQPADPIWLRQPR
                     LRGQPAGVGWGAGRACVVNCECEISRIAPGDVLVTRMAGPALGRVLPLVAGVVAELGG
                     STSHLASLARERCVPMVLGVPGATLQIPEGAQVAVDGVAGIVRWMPQNR"
     gene            8346..8600
                     /locus_tag="AC16CX_19530"
     CDS             8346..8600
                     /locus_tag="AC16CX_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014735.1"
                     /translation="MSQRIRGITDEEATGLAKEIFATSNQLLGRTANLLRILAHSPYL
                     ARWFLPLVAAVRQPSAGAVSDVRLRNLAVLKTSTINGCKY"
     gene            8616..8984
                     /locus_tag="AC16CX_19535"
     CDS             8616..8984
                     /locus_tag="AC16CX_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014736.1"
                     /translation="MLGQALGLKDEEFVALQGDYRTSPLFNEREKAVLAWSEAMTFNT
                     AKRDKTTWDELRRLFTDAEIVEISLACAMFNMINRLNDSFWTELESEEYNRRQHRAVG
                     VTARALEDYACRICAQTPKA"
     gene            9083..10063
                     /locus_tag="AC16CX_19540"
     CDS             9083..10063
                     /locus_tag="AC16CX_19540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3014737.1"
                     /translation="MRPKIFITQPVAESAIERLRAVADVELNPNTLHKVTKSELLEKV
                     PHLDILFCLLHDKVDRDVIAAGQRLRAIASMKITPSDIDVAGATARRIPVTVIAPIVT
                     EATADIHFALLLAVARRVVEGDQLVRAGIFPGAQSAHLLGSWVYGRTIGLIGGGGRIG
                     RAVARRAQGFSMRTLYWTPRRKPESEEREAGLAYVPYDQLLAESDFVSVHAPLTAETR
                     HLVGERELRLMKPTAFLINTARGPIVDEKALIRALAERRIAGAGLDVYENEPEVEPGL
                     LTMKNVVLTPHLGSAVTELRESMANVVVDNIMAVLEGRRPPNCWNAEIYK"
     gene            10147..11136
                     /locus_tag="AC16CX_19545"
     CDS             10147..11136
                     /locus_tag="AC16CX_19545"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR3014738.1"
                     /translation="MAGKAKVVLTDYVWESLDVEKKILAGLAELVPLQTKKPEDFLSQ
                     AADCDALLNTYAGPITADVMAKMPKCRIIARYGIGVDTIDLEAATQVGIIVTNNPTYC
                     IEEVAEHTMALLLACARKVAFYDRMVRSGTWAVPPGKPLFRLVGRTLGLVGFGNIARQ
                     VAVRAAAFGMRVLYADPFVKQGQFAEPGSKVELSDLLRESDFISVHPPLTPQTRGMIN
                     DDALSRMKPTAFLINCSRGPVVNTDALVRALDAKKIAGCGLDTTDPEPLPDPHPLRGR
                     DNVIINPHVAWYSEQAMVGLQAGAPNEVRRVLSGEWPVNVVNKAVKGKNRAGL"
     gene            11245..12225
                     /locus_tag="AC16CX_19550"
     CDS             11245..12225
                     /locus_tag="AC16CX_19550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3014739.1"
                     /translation="MINAAIIGLGWWGKNWVGAVQGKSAKVRFVHGVSKEIDTALPLA
                     EVHGFTLSDDIEHALADPRVDAVLLATPHSLHTDQIVRVAQAGKPVFCEKPLALKRSD
                     AERSVAACAKAGVLLGIGTNKRFWPSMAELRRVVANGALGRVMLIEGHYSNENSGLHF
                     SAWRDSPDEAPGGGMTGTGVHLLDAFVSIAGPVSEVNARLTTLRPGRDPRDVIGVSFD
                     FASGLTGYFGAVRASPVYWRVHVFGDEGSAEALGETQLVLRSRGGKVEVRDFPKVDSI
                     LAELDAFADAVEGRAPYPITTREMVDVVVAFEAIIQSMDTGTPVSISNNR"
     gene            complement(12511..12681)
                     /locus_tag="AC16CX_19555"
     CDS             complement(12511..12681)
                     /locus_tag="AC16CX_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014740.1"
                     /translation="MRVIALIEDPGVIRRILEHLGLWAPLATERSPPLGPASWLRHAS
                     LPLTYHPVPDIA"
     gene            complement(12972..14135)
                     /locus_tag="AC16CX_19560"
     CDS             complement(12972..14135)
                     /locus_tag="AC16CX_19560"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HIR3014741.1"
                     /translation="MQNSLNTSLAVLVVCFCLTGVAEAGPLDDADAAYKRGDYATALR
                     LIRPLAEQGNADAQHNLGVMYDRGRGVPQDYAEAMRWYRLAAEQGNAAAQSNLGHIYS
                     KGKGIPEDYAEAAKWFRLAADQGDVGSQYILGVMNVQGRGVSQDYVQALKWFRLAADQ
                     GAAPAQYEIGSMYRDGRGVRQSDAEAVKWYRLAATKGYANAQFSIGFSYEMGRGVRQN
                     YAEAVKWYRLAADQGYVTAQYNLGVFYSRGLGLRQDYTEAMKWYRLAADQGDALAQSN
                     LGAMYAFGQGVPENQLEALRWFRMAADQGLASAMLNLGVSYETGEGVLQDYVQAHKWY
                     TLAIAHASASETEPRSIADKYRTNLAAKMMPEQIAEAEKLAREWKPKVVGGSD"
     gene            14588..15163
                     /locus_tag="AC16CX_19565"
     CDS             14588..15163
                     /locus_tag="AC16CX_19565"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3014742.1"
                     /translation="MASGAFGRIMLIEGHYSNENSGMHFSAWRESPDESPGGGMTGTG
                     VHPLDAFVSIAGPVSEVNARLTTHRSGPDPRDTIGVSFEFANGLTGYFGAVRASPVYW
                     RVHVFGDEGSAEALGETQLVLRSRGGKVEVRDFPKVDSILAELDAFADAVEGRASYPI
                     KPKEIVDVVAAFEAIIQSMDTGTPVSITPAP"
     gene            complement(15147..16157)
                     /locus_tag="AC16CX_19570"
     CDS             complement(15147..16157)
                     /locus_tag="AC16CX_19570"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014743.1"
                     /translation="MLRGTSVVVLAWLFAACAQPAYGQHNQLIDESVRLSGKGRFAEA
                     AEMAAKALKSAEETLGPEHPEVATALNNLAELQLMSAFVSSKDEATRLKAAAFAEAMH
                     KRALAIREKTLGLRNSFTIASIINVAKAYHLQRRYDDAETWYKHAQGVLNEIGPRAPI
                     MSDLARQATTALNQIDKQRRKIEASAQPQAATEKAYTNPDHRWSISYPADWKLDDNDR
                     FFVKISRGPALVGIHTYTDVAGKSLDEVADAALQRWEQGMRNVNVFKQVSRQRLTLPG
                     DLPAIEIVHHIGRGVIGKSRKVIAVAKDRAFWIDAETNLASWPDYEGDFNRIIESFRV
                     QE"
     gene            complement(16272..18107)
                     /locus_tag="AC16CX_19575"
     CDS             complement(16272..18107)
                     /locus_tag="AC16CX_19575"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR3014744.1"
                     /translation="MNQPATTAHLAQAPAAPAHVKHVKLKAWVAEIAQLTKPDRIYWC
                     NGSQEEYDRLCGEMVASGMLIKLNPQKRPGCFLARSDPDDVARMEERTFVCPRNQEDA
                     GPNNNWVAQDEMRATLGKLFDGCMRGRTMYVIPFSMGPLGSHIAHIGVELTDSPYVVV
                     NMKLMTRIGRKVLDVMGPGGYFVPCVHSVGAPLAPGRKDVPWPSNKQHKYIVHFPETK
                     EIWSFGSGYGGNALLGKKCFALRIASIMAKEQGWLAEHMLILGIQPPKGEKRYIAAAF
                     PSACGKTNLAMLIPPKALKGWKVTTIGEDIAWIKPHSDGRLYAINPEAGAFGVAPGTS
                     EFTNPNCLKAVTHDTIFTNVALTPDGDVWWEGLTQSPPKELTDWQGKPWTPGCGRKAA
                     HPNARFTMRCSQIPSVDPDWENPDGVPISVFLFGGRRATTVPLVTEAFDWTHGVYLAA
                     TMASETTAAIVGEVGVVRRDPFAMLPFCGYHYGDYFRHWLEIGRRLKHPPRIFGVNWF
                     RQDEKGDFIWPGYGENMRVLKWVIERCDGKAKAVKTPIGWQPRYEDLDWNGLDLPRER
                     FEELTKFDPALWRQELKDHAELFEKLKARMPKELLSQREQLERAL"
     gene            complement(18246..19430)
                     /locus_tag="AC16CX_19580"
     CDS             complement(18246..19430)
                     /locus_tag="AC16CX_19580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014745.1"
                     /translation="MEPAKRNVAVLAVCQGLLLINNSVLITVNALAGYALAADKALAT
                     LPVTAYFVGSALTTLPLSFLMKRHGRRSGFTLGASFAVIGSLLCASAVYAGSFWLLCF
                     GVLVFGAYFAAGQYYRFAAADAAPADFKSTAISLVLAGGIAGGFIGPETSKLTKDIVA
                     GHTYVGPYLSLVAFALLSLLAQRWLEIPLLSEAERKDVGRPLGEIARQPVFIVAVLCA
                     MIGYGVMNLLMTATPLAMIASEHPFSDAAFVIQWHLVGMFAPSFVTGSLIRRLGLIPV
                     MLTGVALNMACVAIALAGVDVMHFWLAMLLLGVGWNFMFVGATALLTECHAPAERAKV
                     QGVNDAAIFITMVVSSLSSGALFTQQGWHVMNILAVPFLALAGAGLWWLWPFRERPRR
                     AI"
     gene            19645..>19835
                     /locus_tag="AC16CX_19585"
     CDS             19645..>19835
                     /locus_tag="AC16CX_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3014746.1"
                     /translation="MTESTEKIEIYRGLKGVYFDHSRVCYIDGRAGELRYRGYSIHDL
                     AQRSTFEETCYLLLKGELPT"
BASE COUNT         3783 a         6288 c         6280 g         3484 t
ORIGIN      
        1 ggtccgacct ggaaggggat cgatgggacc ggctacctga tgagtttcag cgacggcgca
       61 atctatcacg gcgtgatttc gtgggcgctg ccggaggtgt ggccgggggc ggtctacagt
      121 ttagagatcg gcgcggtcgg caccgaaggc gtgctcacca tcgacgacac gcaccgcgac
      181 atcgtcctgg caaccaatct gatccaggcc gaaggctatg cgccggacaa gcgccgccac
      241 gtcgacttcc tgaccagcta tccggtcggc gacatggcgc tcggcgagct gcgcggcccc
      301 atgcgcgagg aaaccaattc gtggctgaac cgcgtgtcgc tcggcgtgac gacccagcac
      361 gcaaccgccc gcgaaggtca caatcgcctg atgctgacca aggcgattga tctttcggcg
      421 cggttgaagc gcgcggtcaa gctcccgatc ggtccggagg acatgggcgc tcattgacgg
      481 aacaagacga ctgcagccga ccgccgcatt tcaaatcgaa ggaggaatcc atgaagaccg
      541 aaagaaaata cagcgtgttg ctggccgccg tcctgacggt cggcgcgttc ggtgcgcaag
      601 cggccgagaa gttcccgcag cggccggtgc gtttcatcat cccctacgcg ccgggcgggg
      661 caacggatat caccgcgcgc cagctgcaaa ccaggctgag cgagtcctgg ggccaaccca
      721 ttgttatcga caatcgcgcc ggcgcatccg gcaacatcgc gcttgagctg gcgtcgagat
      781 cggcgcccga cggctatacg ctgttcgtcg gcaacgtctc caccaatgcc atcaacgaaa
      841 ccaccttcgc ctcgctcaag gtcaagccgt cgcgcgacct gaccggcgtc accaacctga
      901 tccagttgcc gcatctgtgg gtcgtgaacc ccggtgttcc cgcttccacc ctcaaggaac
      961 tggtgcaata cgtcaagaag tcgggtgtgc gcctcaatta cggctctgcc ggggtcgggg
     1021 cctatcctca cctggacgcg gtgaagttcc tcaagatcgc cggcatcgag atgacgcacg
     1081 tgccgtacaa gggcggcgcc gggcagatga tccccgcgat catgggcaac gaggtgcagt
     1141 tcatgttcat caacatggcc tcctccatct cgaacatccg cgccggccgg atcaagccgc
     1201 ttgcgataac caccgcggag cggcgtcccg agttgccgaa cgtgcccacc accgccgaat
     1261 ccggcttccc cggcgtcggg accaacgcct ggaacggcct gttcgccccc gccggcatcc
     1321 cgaaaccgct gctcggccgc atccacgcgg atgtcgtgaa ggtcatggaa acccctgaaa
     1381 tgaaggcgtc tctcgccaag gtattcatgg cggtcgtcgt ggacaagtct cccgctgatt
     1441 tccagcaatt cgtgctgaaa gagatcaagg aatggggaaa gatcgtgatc gagaacaaca
     1501 tcaaggtcga atgaaggacg cggtccgcgt ccttcattaa cgagaatcat gaacaaagct
     1561 taccaacccc ggcacgatct cgtcgtcgag aaggacgtcg aggtcccggt gcgcgacggc
     1621 gcccggctca aggccgacat cttccggccg acgtccgccg gccgcttccc ggtgatcatg
     1681 aacctcgggg gctaccgcaa ggacaaggtg tgggtgccgc cgccggacct ggaagagaag
     1741 gccaacccgt acatgaactg ggaaaccgcc aatcccctgt ggtgggtccc gcgcggctac
     1801 atcgcgatcc gggtggacac gcggggctcg ggcaagtcgc ccggtctgtt cgatccgtgg
     1861 tcgcggcagg aagcgctcga ctttcacgac tgcatcgaat gggcggcgaa acagaagtgg
     1921 tgcaacggcc gcgtcggctt gagcggcatt tcctatttcg cgatgaacca gtggctggtc
     1981 gccgggttga ggccccccgc cctcaaggcc atcattccgt gggagggcgc ggcggatatg
     2041 taccgcgatc tcgcctacca cggcggcatc ttctgcctgg gattcatcgg caactggtac
     2101 aacaaccaca tggcgcacca cctgctcggg aaaccgcagc agcacaaccc ggacgccttc
     2161 tccaacaact ggctgtggca gtacatgcgc cacagcctcg acactggctg gttcgaccag
     2221 cgccgcgcgg actggggccg catcgaagtg cccatgctga ccgccggcaa ctggagcggg
     2281 atgggtctac atctgcgcgg caacaccgaa ggcttcatgc gcgccgcctc ccgccacaag
     2341 aagctgagaa tccacgccgg cacccactat catccgttct acagcgagga cggccgccgc
     2401 gaccagttgc gcttcttcga ctactggctg aagggcgaga agaacggcgt gatggacgag
     2461 ccgccggtga agctcctcat ccgcaaaggc ggtgaaggca actacgagtg gcggagcgag
     2521 cgggagtggc agctcaagcg aacgcgctgg atgcgcttct atctgagccc ctcccgggtg
     2581 attgtcagga accgcgagcc ggccgaggga ttgctggtca cggcgccgcc gaggaaaaag
     2641 agcgtcatca cctactccgc ggccgcgatg tccaaggcgg gcgtcgcctc ggcttcatgg
     2701 acctcgacgg cgctcccggg cagcttgccg cggctcggcg tatcgttcga gaccgacgtc
     2761 ctcaaggaag acctcgaggt caccggcccg gtggtgctgg cgctgtggct gtcgagctcg
     2821 accgaggaca tggatctgtt cgcgacgatc cgcaacatcg accgcgaggg catggaggtg
     2881 tgggaggtcg gccagcagca acagcccgtg cccgtggcca agggctggct gcgcgcctcg
     2941 caccggcatc tcgaccgcag cctcactcgc ccctaccggc cgtaccatgc gcatgaccgc
     3001 cgcctgtggt tgaagaagaa cgagccggtc aaggtggacg tggagatctg gccgacctgc
     3061 atggtgtttc gcaaggggca ccgcatccgt ctcgacatcc agccgcgcga cggcgtgggg
     3121 tccgccccgt acacgcatta ctgcgcggat tacaatgtcg gcgacaacac aatatacacc
     3181 gggggcacca tggcatccca cctgctgctg ccggtgatcc cgcagaaata accggcacaa
     3241 caaccacgag gagaacaaca tcatgaggcc tctaagcttc ctgctctcag ccctgtttgc
     3301 tgcatgcctg ctcgcgagtg cgcaagcctt tgcccagaag gcgaaggacg actacccgtc
     3361 gcggccggtg cgcatgatcg ttccgttcgc gccgggcggt gcctcagact tcgtcggccg
     3421 catcatccag ccggcgatgg ccgatctcct caagcagcag gtggtggtgg acaaccgcgg
     3481 cggcgccgcc ggcaatatcg gggtggaagt cgcagtccgc gccacccccg acggctacac
     3541 gtttctgctc ggcaacgtcg gcacgatggc gatcaacccg gtctattaca ccaagtttcc
     3601 gtaccggccg ctgaaagacc tggtgccaat tacccaggtg gtggatgtgc caggctgcct
     3661 ggtggttcac ccttccttgc ccgcgaaaaa cgtcaaggaa ctgattgcgc acctgaaagc
     3721 caacccgggc aagctcaact acggcgcgcc ggcgccgagc agcgcgaacg ccctggaaat
     3781 gcacatgttc cttggcgcca ccggcacgaa cgcggttcag gttgcgtata aaggcggcgc
     3841 cgggccggcg atgatcgggc tcctgggcaa cgaggtgcag atgatgttcg tgacgttctc
     3901 ctctgcggtc aacttcgcca agcagggccg ggtccggatg ctgggcgtaa tttcgcccga
     3961 gcgcaacccc gcccttcccg aaatcccgac gatgcgagag cagggtctcc aaatggtcgt
     4021 tgggtcgtgg cagggcctgt tcgtaccgaa gggcacgccg ctgccggtgg tcaacaagct
     4081 ctacaaggtc ggcatcgaga tgatgaagga cccgcaggtt atcaagcgcc tctccgatgg
     4141 cggggtcacc atcgtcacca gcaagtcgcc ggcggacttc gtcgccttcg tgaagaagga
     4201 aaccgagcgc ttcggaaagg tgatcaagga cgcgaagatc ccgacggaat agtacccggc
     4261 gcgcagtgaa ggcattttag ccgccgatga acgcagataa gacccgggaa gacgaatagc
     4321 cgccgatacg cgcagctaca cgcggatcag acgatcgacc aagggtcgcc gatagactcc
     4381 cagccgaacg tcattcaagg agcgggcgga ttatttgcac aggatttgat ctggatcagg
     4441 gacatctgtg ttcatctgcg cttatctgcg actggttctt gattgaattt ttcgagagaa
     4501 ggtctgaaaa cgcgaacagg agagcgagat ggcaaatgtc ggaatggtgg gattggattg
     4561 gcaacagcgc atcaattggg accgtctgcg caagtaccgc accgaacggg cgcgggagcg
     4621 gatgaaggcg cacggattga gcgcgatgct ctgcatgtat gacgagaacg tccgctacat
     4681 caccggcacc cttacccccg gctggaaccg gctcaagccc ggactgcgct actgcctgct
     4741 gtgcggcgat gatgcgccgg tgctgttcga acaaggggac atcggcgcgc aggtcaaccg
     4801 ccattcgccg tggctcccga aggaccacgt gcgctggtct tacgcctgga tcaagggcgc
     4861 ggcgggcccg gcgtccaccc agcaggtgaa aaaattcacc aacgggatca agcaggagat
     4921 gaagaaggcg aacgtcgcga acggcaagct gggtgtggac ttcatcgaca tcaacatgat
     4981 ccagatcttc aaggacgaga agatcgactg gaccgacggc atgacggcga tgatggaggc
     5041 gcgcgcggtc aagaacgagg acgagcagga ggcgctgcgc atcgtcgggg ccattggtga
     5101 cgccgcgcac tgggaattca tgaagttcct caagcccggg cgcaccgaaa accagatgac
     5161 cgcgcgcatc atggagttcc tctactccat tccggggatg gaggacgtcg aggacgtgat
     5221 cgtttcctcc gggcccaata cctggcccaa ctggcgcaat ttctccgatc gcatcatcca
     5281 gcccggcgac atcgtcttca tggatctcgc cgcgctcacg tggaacggct acaagtcctg
     5341 ctactaccgc acctactgcg cgggcaagga gccgacgaag gagcaaaagg acgtctacgc
     5401 gaccgcgctc aagtggctct acgattccat gaaggccgtg aaggtcggaa ccacgacgcg
     5461 cgagatcgcc tcgaagtggc cttcagccaa ggaggcctgg ggctatgccg aggaggacca
     5521 ggcggcggcc aacctgtggg ggcacggtct cggtctcgcc caatacgacc agccggtgat
     5581 ctcccgcatc tggtcgctcg atcatccggt cgagatcaag gaaggcatgg tattcgcgct
     5641 ggagacccag cacggcaagc agttcgaatg gggggtgcgc atcgaggaga tgctgatcgt
     5701 gcacaaggac cgcgtcgagc tgatctccaa cttcccggtc gagcagatca ccgtcgtcga
     5761 tcccatgccg ggctattcga cgctcgggtg gtctaggtag gagatggggc ggtatcctca
     5821 acaatcactg ttggagggaa ctatgaacat gcggatctgg atcgcggtgg cagccgcagc
     5881 gcttgcggcg gtgctttcag gcgggtgcgc gacggctccg cagccgccgg tcgcgatgga
     5941 agcggtcatc ggcaacaact tcggccatct gccgatgttc gtcggcgtcg agaaggggct
     6001 gttcaagaag cacggcatcg atctcaagct caaggtcgtc aacaccggca ccgacatggt
     6061 gaacgcgatg accaggcacg aggtgcagat cggcgacatg agcgtcacca cgttcctcaa
     6121 ggcgcggcat gcgggctcgc cgttcctggt cgtcggcatg atcatgaacg acgccacgac
     6181 caccttcgcc gacagcccgc tcgcgatcgt cgcgagaaga ggttcgggca tcacgcgggt
     6241 cgaggacctc aagggcaagc gcatcggcct ggcgaaggag cagacctccg acgagtacct
     6301 gaagatggtg ctcgcccgcc gcggaatgca gtacggcgag atgaacatcc agaacatcat
     6361 ggcgccgccg gcgctggtca gcgcattcgc ggacggcaag ctcgacgcga tggtgtcgtg
     6421 ggagccgttc aacaccatgg gcctgatgcg cacgccggag tcgtacgagg tgctgcgcgg
     6481 cggcggccat ctttcctaca tgatgatcgc cacggtccac gacccgatgg tgcaggagaa
     6541 cccgaagctg atccaggcgt tcgtcaacgg gctggcggcc gcgtcccatt acacgcggca
     6601 gaaccgggcc gaggcggtgc agatcttcgc gaagtgggtg ccgaacgtga acctcgaggt
     6661 cgcgcgcaag gcgatccagc acatcagcta cgatccccgg atatcacggc agagcttgcg
     6721 tgcgttcgag gatgcgcaga acgagctgct caagctcacc atcaagggcg cgaagccgct
     6781 gaggatcacc gacatcgtac tcacgtccta caccgaggcc gcggagcggg cttatccgga
     6841 gtacttcgcg gacctgccgg cgctccctcg ctagttcagg cgcggcgggc gggaagcgga
     6901 gaccgatgac tgaaacgaca cggatggcgc tcgccgggat ctcggaaatg ctcatcatcc
     6961 ccctctccgc tcccgaggcg accgatgcgg ctcgtgttgg tcccaaggct gcgaatctcg
     7021 ctgcgctggg tcgggccggg ctgccgatag cggacggttt ctgcgtcact gccgacgctt
     7081 atcgtatgca gattgcggcg ctgaggctgg aacgcacaac gcgcgaagta ttttcggccg
     7141 aggacgcgca ggcgcgccgc agcgccctgc agctgcgcat gcggctgatg gagcagccca
     7201 tcgttgcgga agcaacggaa cccctcctcg gggcatggcg gaccatcacc cggcacggcg
     7261 cgctgccggg cgtggtgcgc tcgtcggcgc tggtcgagga tcgcttcggg tcgagtttcg
     7321 cgggccagtt cgaaagcttt ctggggctgg acaatgaaac cgacttcctc accgcggtgc
     7381 gcgcctgctg ggcggcgctc tggtcgccgc gcgcgttgcg ttacatggcc acgcacgacg
     7441 tcgagccggc gaacacggcc atggccttgc tgatccagcc gctcgtggcg gcgcatgcgg
     7501 caggcggcgg attgagccag accgccgagg gcgggatgct gatcaacgca acacccgggc
     7561 tcgggtgcgc gatcgcgcag ggcgaggttg cgcccgatcg ctacgcgctc gaacggaacg
     7621 gtgccctgcg cgaaagccgc gcggggatgc agtaccacca ggcgagctgc gtccatcagc
     7681 cgcgcctgcc gcgaccgcct gcagcgaaga aactgtgcct cacgccttcc cgggcggaag
     7741 agttggggcg ctttctgctc aaagccgagg cggtcatggg catgccggtc gaaatcgagt
     7801 gggcgctcga cgacacggga ttcaagctgc tgcaggcgcg cccgctgcat ctcgacgaca
     7861 agccttcatc ccagccagcc gacccgatct ggctgcgcca gccgcgcctg cgaggccagc
     7921 cggcgggcgt gggctggggc gcgggccgcg cctgcgtggt gaactgcgag tgcgagatct
     7981 cgcgcatcgc gccgggcgac gtgctcgtga cgaggatggc cgggccggcg ttgggccgcg
     8041 tgctgccgct cgttgcgggc gtcgtcgccg agctcggcgg cagcacctcg cacctcgcct
     8101 cgctcgcgcg cgagcgttgc gttcccatgg tgctgggtgt gcccggcgcc actcttcaga
     8161 tccccgaggg cgcgcaggtt gcggtagacg gcgtcgcggg aatcgtgcgc tggatgcccc
     8221 aaaaccggtg actaagtgat taggtgatta agtgattgcg caaccccgcg cgttaatccc
     8281 gtttggcgct aatcacccag tcacttagtc actcaatcac ctagtcactc agtcactcaa
     8341 ttcatatgag ccagcgaatc cgcggcatca cagatgaaga agcgacgggc cttgcgaagg
     8401 aaatcttcgc gacgtcgaac caattgctgg gccgcacggc gaatctcctg cgcatactcg
     8461 ctcatagccc gtacctggcg cgctggttcc tgccgctggt cgccgcggta cgccagccga
     8521 gcgccggcgc cgtgtccgac gtgcggctgc ggaacctggc cgtattgaag acttcgacga
     8581 tcaatggctg caagtactga acggaacaca atcgggtgct tggtcaagca ctgggactga
     8641 aggacgagga gttcgtcgcg cttcagggag attaccggac aagcccgctc ttcaacgaac
     8701 gggagaaagc ggttctcgcc tggtcggaag cgatgacgtt caacaccgcg aaacgcgaca
     8761 agacaacatg ggacgagctg cggcggctct tcaccgacgc cgaaattgtc gaaatctcac
     8821 tcgcctgtgc gatgttcaac atgatcaacc ggctcaacga ctccttctgg accgagctcg
     8881 agtccgagga atacaaccgc aggcagcatc gcgcggtcgg cgtgacggcc cgggcgctcg
     8941 aggactacgc ctgccgcatc tgcgcacaaa cgccaaaagc gtgattggtg attcgtgatt
     9001 ggtgattgga cgagccactg ccgatacgcc tttttccatt cactattcac cattcactat
     9061 tcacgccttt gagtagctgc cgatgcgccc gaagatcttc atcacccagc cggttgccga
     9121 gagcgcgatc gagcgactgc gcgccgtcgc cgacgtcgag ctgaatccca atacgctgca
     9181 caaggtcacc aagagcgagc tgctcgagaa ggtcccgcat ctcgacatcc tgttctgcct
     9241 gctccacgac aaagtggacc gcgacgtcat cgcggccggc cagcgcttgc gcgcgattgc
     9301 ctcgatgaag atcactccgt ccgacatcga cgtcgccggg gccaccgcgc ggcgcatccc
     9361 ggtcacggtc atcgcaccca tcgtgaccga ggcgacagcc gacattcact tcgcgctgct
     9421 gctcgcggtc gcccggcgcg tggtcgaggg cgaccagctc gtgcgcgctg gaatcttccc
     9481 cggcgcgcag tcggcgcacc tgctgggctc ctgggtctac ggcaggacca tcggcctgat
     9541 cggcggcggc gggcgcatcg gtcgcgcggt ggccaggcgc gcgcagggtt tctcgatgcg
     9601 cactctctac tggacgccgc gccgcaagcc tgaaagcgag gagcgcgaag ccggcctcgc
     9661 ctacgtgccg tacgatcagc tgctcgccga gtccgatttc gtctcggtcc acgcgcccct
     9721 caccgccgag actcgccacc tggtcggcga gcgcgagctg cgcctcatga aacccaccgc
     9781 cttcctgatc aatacggcgc gcggcccgat cgtggacgaa aaggccctga tccgtgcgct
     9841 cgccgagcgc cgcatcgcgg gcgccggcct cgacgtttac gagaacgagc cggaggtcga
     9901 acccgggctg ctcaccatga aaaacgtcgt gctcacgccc catctcggga gcgctgttac
     9961 ggagctgagg gaatccatgg ccaacgtggt ggtggacaat atcatggcgg tgctggaggg
    10021 ccggcggcca cccaactgct ggaacgcaga gatctacaag tgactcgttt ctcgttactc
    10081 gttcctcgat gtcagccggg aaaggcgagc aacgagtaac gagtcactct ttcaagaggt
    10141 gctgaaatgg ctggaaaagc taaagttgtc ctgaccgact acgtctggga atccctggac
    10201 gtggagaaga agattctcgc gggccttgcc gagctggtgc ccctacagac gaagaagccg
    10261 gaggatttcc tgtcccaggc ggcggactgc gacgcgctcc tcaacaccta tgccggcccg
    10321 atcacggccg acgtgatggc gaagatgccc aaatgcagga tcatcgcccg ctacggcatc
    10381 ggcgtagata cgatcgacct ggaggccgca acccaggtcg gcatcatcgt caccaacaac
    10441 ccaacctact gcattgagga agtcgccgag cacacgatgg cgctgctcct cgcctgcgcg
    10501 cgcaaggtcg ccttctacga ccgcatggtg cgttcgggga cgtgggccgt gccgcccggc
    10561 aagccgctgt tccggctcgt cgggcgcacg ctcgggctgg tcggcttcgg caacatcgcc
    10621 cgccaggttg ctgtgcgcgc ggccgccttc ggcatgcggg tgctctatgc cgatccgttc
    10681 gtgaagcaag gccagttcgc cgagccgggc tcgaaagtgg agttgagcga cctgctgcgg
    10741 gagtcggatt tcatctcggt gcatccgccc ctgacgcccc agacccgcgg catgatcaat
    10801 gacgatgcgc tctcgcgcat gaagccgacc gctttcctga tcaattgctc gcgcggcccg
    10861 gtggtgaata ccgacgcgct ggtgcgggca ctcgacgcga agaagatcgc cggctgcggg
    10921 ctcgatacga ccgatcccga gccgttgccg gacccgcacc cgctgcgcgg ccgggacaac
    10981 gtgatcatca atccccacgt cgcgtggtac agcgaacagg ccatggtcgg tctgcaggcc
    11041 ggcgcgccga acgaggtgcg ccgcgtgctt tccggcgagt ggccagtgaa cgttgtcaac
    11101 aaggcggtca aagggaaaaa ccgcgcaggc ctctaatctt gtgattaagt gattgagtga
    11161 ctaagtgatt gagtaaaacc gcacgacacc gctccgtctc acctagtcac ttaatcactt
    11221 agtcacttaa tcactggggt cataatgatt aacgccgcca ttatcggact gggctggtgg
    11281 ggaaagaact gggtcggcgc ggtccaggga aagagcgcga aagtgcgctt tgtccacggc
    11341 gtgagcaagg agatcgacac cgcgctgccg ctcgcggagg tccacggctt tacgctgagc
    11401 gacgatatcg agcacgcgct cgccgaccct cgcgtcgacg ccgtgctact cgcgactccg
    11461 cactctctgc acacggatca gatcgtacgc gtggcgcagg ccggcaaacc ggtattctgc
    11521 gagaagccgc tcgcgctgaa acggtccgac gccgagcgct cggtcgccgc ctgcgcgaaa
    11581 gccggagtcc tgctcggcat cggcacgaac aagcgtttct ggccgtcgat ggcggagctg
    11641 cggcgggtgg tggcaaacgg cgcgctcggg cgcgtcatgc tgatcgaagg gcactacagc
    11701 aacgagaact ccggcctgca cttctcggcc tggcgcgatt cgcccgacga ggctccgggc
    11761 ggtggcatga ccggcaccgg cgtccacctg ctcgacgcgt tcgtaagcat cgccggtccc
    11821 gtgtcggaag tgaatgcgcg gctcaccacg cttcgcccgg gccgcgatcc ccgcgacgtc
    11881 atcggcgtca gcttcgactt cgccagcggc ctgaccggct acttcggcgc ggtgcgcgcg
    11941 tcacccgtct actggcgcgt gcacgtgttc ggtgacgaag gctccgccga ggcgctgggc
    12001 gagacgcagc tcgtgctgcg cagccgcggc ggcaaggtcg aagtgcggga ttttccgaag
    12061 gtggactcta tcctcgccga gctggatgcc ttcgccgacg ccgtcgaggg ccgggcgccc
    12121 taccccatca cgacccgtga aatggtggac gtggtcgtgg cgttcgaagc gatcatccag
    12181 tccatggaca ccggcacgcc ggtgagcatc tcaaacaaca gataggaaac aacagaacag
    12241 ataggaacag ataagaaatt caatccggcc gcgatcccgc ctcgccgatc cgaggcgccg
    12301 ggagccggtg cgcaagaggt ggcgcaatgc gcataattgg ccttatgtcc aagccggcgt
    12361 cctcccccct gtgaaatgac cgcgatccgg gcgggtcgtg agccggatca gcggtcacac
    12421 caatcaattt attccagttg tcagagcgag gcaagccccc tccccgcgcc tcgactgttc
    12481 agtgaagcgc cggttcttcc ttactgcagg tcaggcgatg tcgggaaccg ggtgataggt
    12541 cagtggcagg ctggcatgcc ggagccaact cgcgggacca agcggcggac tgcgctcggt
    12601 ggcgagcggc gcccacaatc cgaggtgctc gaggatgcgc cggatgactc ccggatcctc
    12661 gataagcgcg atgacccgca tcggcccctt gcactggggg cactccaaca gggaaatggc
    12721 cgggcattcc tgcgcacgat taacgcagga atccaggcca ttgataattc tggtagcggg
    12781 gggacgattg aacctatctc acgcgtgcgg attcccgtga gaaaacaggc cgtttgacct
    12841 tggaaaatcg gaaccccgac gcgaggccgg ggttcttgaa agcggtagcg ggggcctgtt
    12901 ttccgcttcg acactgcgag atcgtgccgc ccagaaacag aatccccggc gcgcggtcga
    12961 ggatcgtgaa tctaatcgct gccacctact accttcggct tccactcgcg ggccagtttt
    13021 tctgcctcag cgatttgctc cggcatcatc ttggcggcca gattggtgcg atacttatcg
    13081 gcgatcgaac gcggctcagt ttccgatgct gaagcgtggg caatcgccag cgtgtaccat
    13141 ttatgcgcct ggacgtagtc ctgcaacacc ccctcaccag tttcataact gaccccgagg
    13201 tttaacatgg ctgaggccaa tccctgatca gcagccatcc ggaaccagcg taacgcttcg
    13261 agttggttct ccggcacgcc ttgaccgaat gcatacatgg cgccaagatt gctctgagcg
    13321 agcgcgtcgc cctggtcagc agcaaggcga taccacttca tcgcttcggt atagtcctgt
    13381 cttaggccca aacccctgga ataaaatacg ccgagattat actgggctgt cacatagcct
    13441 tggtcggccg ccaggcgata ccacttgacc gcttccgcat agttctgcct cacgcctctg
    13501 cccatttcgt agctgaatcc gatgctgaac tgcgcgttgg cgtagccttt ggtggccgca
    13561 agccgatacc atttgactgc ctcggcatcg ctctgccgca ctcctcttcc atcgcggtac
    13621 atggaaccaa tttcgtactg ggccggagcg gcgccttgat cggcggccag tcggaaccac
    13681 ttcaatgctt gaacgtagtc ttgactcact ccccttccct ggacattcat aacaccgagg
    13741 atgtactgcg agccaacatc gccctggtca gcggcgagac gaaaccattt cgccgcctcc
    13801 gcgtagtctt ccggtatccc cttgcctttg ctgtagatgt gaccgaggtt ggactgtgcc
    13861 gcggcgttac cctgctcggc agccaggcga taccacctca tggcctcggc gtaatcctgc
    13921 gggacaccgc ggcctcgatc gtacatgact ccgaggttgt gctgcgcgtc ggcgttaccc
    13981 tgctcggcca aggggcgaat gagccgcaag gccgtcgcgt agtcgccgcg cttgtatgcg
    14041 gcatccgcat cgtcgagagg acccgcttct gccactccgg tcaggcaaaa acagactacg
    14101 agaaccgcga gggaagtatt gagcgagttc tgcatggctg accgcctctc cgacgcacgc
    14161 gtaggaagga taggaaattc aaaggataga aggataggaa attcaaagga taggaaagga
    14221 taaaaggata aaggatagga aattcaatcc gcccgggatc gcgccgggcc aggtcgaggc
    14281 gccgggagta agtgcgagag ggttcccgga ccgcgcataa cagaccttat gtccaagccg
    14341 gcgtcctccc ccctttgaaa tgaccgcgat ccggacgggt cgtgagcagc atcggcggtc
    14401 aatcatcgat caatttattc cagttgccac agcgaggcaa gccccctccc cgcgcctcga
    14461 ctgttcagtg aagcgccggt tcttccttac tgcaggtcag gcaatgtccg gaaccgggtg
    14521 ataggtcagt ggcaagttgg catgccgcgg ccaactcgcg gcgggaccgg gcggtggact
    14581 tcgctcggtg gcgagcggcg cgttcggacg gatcatgctc atcgaggggc attacagcaa
    14641 cgagaattcg ggcatgcact tctccgcctg gcgcgaatcg ccggacgagt ctccgggcgg
    14701 cggcatgacc ggcaccggcg tccacccgct cgatgccttc gtcagcatcg ccggcccggt
    14761 gtcggaggtg aacgcgcggc tgacgacgca ccgctccgga ccggatcccc gcgacaccat
    14821 cggcgtcagc ttcgagtttg ccaacggcct caccggctac ttcggcgcgg tgcgcgcgtc
    14881 gcccgtctac tggcgcgtgc acgtgttcgg tgacgaaggc tccgccgagg cgctgggcga
    14941 gacgcagctc gtgctgcgca gccgcggcgg caaggtcgag gtgcgggatt ttccgaaggt
    15001 ggactctatc ctcgccgagc tggatgcctt cgccgacgcc gtcgaggggc gggcttccta
    15061 cccgatcaag ccgaaagaga ttgtagacgt ggtcgcggcg ttcgaagcga tcatccagtc
    15121 catggacacc ggcacgccag tcagcattac tcctgcaccc tgaacgactc gatgatccga
    15181 ttgaaatcgc cctcgtaatc gggccaggaa gcgagattcg tctcggcgtc gatccagaac
    15241 gcgcgatcct tggcgacggc aatgaccttt cgcgatttgc cgatcacgcc cctgccgatg
    15301 tgatgcacga tctcgattgc gggcagatcg cctggcaagg tcaggcgttg ccgcgagact
    15361 tgcttgaaga cgttcacgtt tcgcatgccc tgctcccacc tttgaagggc ggcatcagcg
    15421 acttcatcca gggatttgcc ggcgacgtcg gtataggtgt ggattcccac cagcgccggg
    15481 cccctgctga tcttcacgaa gaagcggtca ttgtcatcga gcttccagtc cgcagggtag
    15541 gaaatcgacc agcgatggtc cggatttgta tacgcctttt cggtcgcggc ctggggctgc
    15601 gcggatgcct cgatttttct ccgctgttta tcgatctggt tcaaagctgt cgttgcctgc
    15661 cgtgcaaggt cactcataat aggggcgcgc ggcccgattt cgttgaggac tccttgggcg
    15721 tgtttatacc aggtctctgc gtcatcgtac cggcgctgga ggtggtatgc cttggctaca
    15781 ttgatgatgg atgcgattgt gaaagaatta cgaagaccga gcgttttttc cctgatcgcc
    15841 aaagccctct tatgcatcgc ttcggcaaaa gccgctgcct tgagccgtgt cgcctcgtct
    15901 ttgctggaaa caaaagccga catcagctgg agttccgcta gattgttgag cgcggtagcc
    15961 acttcggggt gctccggccc taacgtctcc tcggcgctct tgagtgcttt ggcggccatc
    16021 tcagctgctt ccgcaaagcg tccttttcca ctgagtctga cagattcatc tatgagctga
    16081 ttgtgctgtc cgtacgcagg ttgcgcgcag gctgcaaaca gccatgcgag tacaactacg
    16141 gaagtgccgc gcaacatggc ggtctcgttt atggatatgc gattgtatat caacgcatat
    16201 atagcgttcg gtggtgcgtg tacatgaaaa agcgggccat gccgcccgct tttccgccaa
    16261 atcgggccag gttacaacgc gcgctccagc tgctcgcgct gggagagcaa ttccttcggc
    16321 atgcgcgcct tgagcttttc gaacagctcg gcatggtcct tcagttcctg gcgccagagg
    16381 gccggatcga acttcgtcag ctcctcgaag cgctcgcgcg gcaggtcgag cccgttccag
    16441 tcgaggtcct cgtagcgcgg ctgccagccg atcggcgtct tcaccgcctt ggccttcccg
    16501 tcgcagcgct cgatgaccca cttcagcacg cgcatgttct cgccgtagcc gggccagatg
    16561 aagtcgccct tttcgtcctg gcggaaccag ttcactccga agatgcgcgg cgggtgcttg
    16621 agcctgcggc cgatttcgag ccagtgcctg aagtaatcgc cgtagtggta gccgcagaac
    16681 ggcagcatcg cgaacgggtc gcgccgcacc acgccgacct ctccgacgat cgccgccgtc
    16741 gtctccgaag ccatggtcgc cgccaggtac accccgtgcg tccagtcgaa cgcttcggtc
    16801 accaggggca cggtggtcgc gcgccgcccg ccgaacagga acacgctgat cggcacaccg
    16861 tccgggtttt cccaatccgg atccacggac gggatctggc tgcagcgcat ggtgaagcgc
    16921 gcgttgggat gcgccgcctt gcgcccgcag cccggcgtcc agggtttgcc ctgccaatcg
    16981 gtgagttctt tcggcggtga ctgggtcagg ccttcccacc acacgtcgcc atcgggcgtc
    17041 agcgcaacgt tggtgaagat cgtatcgtgg gtcaccgcct tcaggcagtt cgggttggtg
    17101 aactcggagg ttcccggcgc cacgccgaac gcaccggcct cgggattgat ggcgtagagc
    17161 cgtccgtcgc tgtgcggctt gatccaggca atgtcctctc cgatcgtggt gaccttccag
    17221 cccttgagcg ccttcggcgg gatcagcatc gcgaggttgg tcttgccaca cgcgctcggg
    17281 aacgccgcgg cgatgtagcg cttctcccct ttcggcggct ggatgccgag gatgagcatg
    17341 tgctcggcga gccagccctg ctctttcgcc atgatggaag cgatgcgcag cgcgaagcac
    17401 ttcttgccga gcagcgcgtt gccgccatag ccactgccga acgaccagat ctccttggtc
    17461 tcgggaaagt gcacgatata cttgtgctgc ttgttgctcg gccacggcac gtctttgcgc
    17521 cctggagcga gcggcgcgcc caccgagtgc acgcagggca caaaataacc gccgggaccc
    17581 atcacatcga ggaccttgcg gccgatgcgc gtcatcagct tcatgttgac gacgacgtac
    17641 ggcgagtcgg tcaattcgac gccgatgtgg gcgatgtgcg agccgagcgg gcccatgctg
    17701 aaggggatca cgtacatcgt gcgcccgcgc atgcagccgt cgaacagctt cccgagcgtt
    17761 gcacgcatct cgtcctgcgc cacccagttg ttgttggggc ccgcgtcctc ctggttgcgg
    17821 ggacacacga acgtgcgctc ctccatgcgc gcgacatcgt cggggtcgga gcgcgcgagg
    17881 aagcagccgg ggcgcttctg cgggttgagc ttgatcagca tgccgctggc gaccatctcg
    17941 ccgcacagcc ggtcgtactc ttcctgcgag ccgttgcacc agtagatgcg gtccggcttg
    18001 gtcagttgcg cgatttccgc aacccatgcc ttgagcttga cgtgcttgac gtgggcgggc
    18061 gctgcggggg cttgggcgag atgggccgtg gtggcgggtt gattcatacg gatcctctct
    18121 ccggcacgat atgaatttat cttttaaaaa caaaggcaaa gcaaccactc cctgatcgcc
    18181 ttgccatttc gccgaattct acacgaaatc catgacgaat ggattgcgcg tcgcgcagcc
    18241 gcgggtcaga tcgcgcgtcg cggccgctca cgaaagggcc agagccacca cagccccgcc
    18301 ccggcgaggg cgaggaacgg cacggcgaga atgttcatca catgccagcc ctgctgtgtg
    18361 aacagcgcac ccgaggacag cgagctgacg accatggtga tgaagatcgc cgcgtcgttc
    18421 acgccctgca ccttggcgcg ctcggcgggt gcatggcact cggtgagcag cgcagtggcg
    18481 cccacaaaca tgaagttcca tcccacgccc agcagcagca tcgcaagcca gaaatgcatg
    18541 acatccaccc ctgcgagcgc aatcgcaacg caggccatgt tcagtgcgac gcccgtcagc
    18601 atgaccggga tcaggccgag gcgccggatc aggcttccgg tcacgaacga cggcgcgaac
    18661 atgccgacca ggtgccactg gatgacgaac gcggcgtcgc tgaacgggtg ctcgctggcg
    18721 atcatggcga ggggggtcgc cgtcatgagc aggttcatca cgccgtagcc gatcatcgcg
    18781 cacagcacgg cgacgatgaa aacgggctgg cgcgcgatct cgccgagcgg ccgcccgaca
    18841 tccttgcgct cggcttccga caacagcggg atctcgagcc agcgctgcgc cagcagggac
    18901 aacaacgcga aggcgaccag cgagagatac ggacccacgt acgtatgacc tgcgacgatg
    18961 tccttggtga gcttgctggt ctcgggtccg atgaagccgc cggcgatgcc gccggccagc
    19021 accagtgaga tcgccgtgct cttgaaatcc gccggcgcgg catcggcggc ggcaaaacga
    19081 tagtactgcc ccgccgcgaa ataggcgccg aataccagca cgccgaagca cagcagccag
    19141 aagctgccgg catagaccgc gctcgcgcac agcagtgagc cgataaccgc aaacgaggcg
    19201 cccagcgtga aaccggagcg gcgaccgtgg cgcttcatca ggaacgacag cggcagcgtc
    19261 gtcagcgccg agccgacgaa gtacgccgtc accgggaggg tcgccagcgc cttgtcggcc
    19321 gccagcgcgt agcccgcgag cgcgttgacc gtgatcagga ccgaattatt gatgagcagg
    19381 aggccctggc agaccgccag cacagcgacg ttgcgtttcg ccggttccat tcacggtgag
    19441 ggaatagggt tgatcggcat gccgcggata cgcggcgcct gcgccacatg cggggaagct
    19501 gcgcggattc cgaagcttcc catacgcccc gcttccggtc aagaataggc gggtcctccg
    19561 gacatccgcg ttccgtcttc cgataatttg tgcaggtgac gcgcgtgccg ggcgaccgga
    19621 gcgtctcgca tcgaaaggac agcaatgacc gaatcaacgg aaaagatcga aatttaccgc
    19681 ggactgaagg gcgtctattt cgaccactcg cgagtgtgct acatcgacgg gcgcgccggc
    19741 gagctgcgct atcgcggcta ctcgatccac gacctcgcgc agcgctcgac cttcgaggag
    19801 acctgctacc tgctcctcaa gggcgagctg cccac
//
LOCUS       DBNGWZ010000625         5068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_477671, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000625 DBNGWZ010000000
VERSION     DBNGWZ010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_477671"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC16CX_19590"
     CDS             <1..245
                     /locus_tag="AC16CX_19590"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR3014747.1"
                     /translation="VRDPVKVAYLRFCDKLRRKGLPRAPAEGPVDYARRIERSRPELQ
                     PAVAAITRLYIALRYGTGAGAGAFDELQQRVRQFGA"
     gene            complement(343..1251)
                     /locus_tag="AC16CX_19595"
     CDS             complement(343..1251)
                     /locus_tag="AC16CX_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3014748.1"
                     /translation="MIRPSLTNVLACALTVAILAGCVARRGAPVVDRSPEARPAPAKP
                     AVTAKPAPKPADARPELYTVRRGDTLYSIALDHGLDYRELAQWNDITDPGVIRTGQQL
                     WLRPPASMATAAPFKTVPGIEGRPLGDAPIAGDNVKAEPKAVRAPYSDQAYAQLAGIK
                     PEPAPKTEPRLPAGGDEGNESIQWGWPASGRVISTFNDSANLKGIGIAGKPGQAVLAS
                     APGRVIFSGTGIRGFGKLIVIKHNNTFLSVYAHNKELLVKEGQNVARGQKIAEMGSTD
                     TDQVKLHFEIRRFGKPVDPMKLLPPA"
     gene            complement(1320..1952)
                     /locus_tag="AC16CX_19600"
     CDS             complement(1320..1952)
                     /locus_tag="AC16CX_19600"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR3014749.1"
                     /translation="MTSQRTRLRMVERLRGQGIRDETVLAIMGELPRHIFVDEALASR
                     AYEDMSLPLGFGQTISSPHTVARMTELARNGRPLAKALEIGTGCGYQTAVLARVARQV
                     YSMERIAALIRKARKHLREAGVSNVHLRHGDGRVGMPEVAPFDAIVMTAAAASAPAAL
                     VEQLAVGGRMILPMAGRNKQYLYVIERTDKGLVERRMDPVNFVPLLPGVG"
     gene            complement(2122..2871)
                     /gene="surE"
                     /locus_tag="AC16CX_19605"
     CDS             complement(2122..2871)
                     /gene="surE"
                     /locus_tag="AC16CX_19605"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR3014750.1"
                     /translation="MRILLSNDDGYFAPGITLLAEVLAPLAEITVVAPERDRSGSSNS
                     LTLDRPLTVRRAQGRFYYVNGTPTDCVHLAVTGLLKELPDIVISGINHGANMGDDTIY
                     SGTVAAATEGFLLGIPSIAISLVAEGGDHFRTAGRVALELVSRFQRQPLGQPVLLNVN
                     VPDVEYAQLRGFQVTRLGRRHKAEPVVKSTTPRGETVYWIGAAGGAQDAGEGTDFHAI
                     AGNRVSITPLQVDLTRYSQFEAVSAWLAGQG"
     gene            complement(2981..3544)
                     /locus_tag="AC16CX_19610"
     CDS             complement(2981..3544)
                     /locus_tag="AC16CX_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3014751.1"
                     /translation="MGYSLQDFCRDTRAILKTGSSRKHVEKVKTYMERLLVDSGFVKE
                     YFGDQQPTGLKRIYTDPRLGFEIMTYRYDEARKSNPHDHGDSWAIYGQVAEYTEMTEW
                     DRIDDGTDPDHARLKPRPSYRLTPGKAGVYYGRELHSTATPVNTRYLRITGTDLENIE
                     RLRIDAATGKIQRIYGRQTGAPAAIVG"
     gene            complement(3670..4575)
                     /locus_tag="AC16CX_19615"
     CDS             complement(3670..4575)
                     /locus_tag="AC16CX_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR3014752.1"
                     /translation="MSGETIAVFTKNRTNPAYEAARLGADRTARRYGARTVHFVPDKP
                     DDVDEQVALVERAVAEHPDAVVFVPVHATALDASVRGLNAAGIPVVNYLNRLAEGRFV
                     SFVGSDDYRLARDIAAYLFRHLGGKGDVVVIEGTPGSVTSRERVRGFRDEAKQWPDVR
                     IAATRAGDYQYETAKKAMAGLLATLPRIDGILSANDVMSLGILSALDAAGRRIPLIGV
                     NAIPEAITAIKEGKLLATVDFDAMKISCIATEAAIRHLGGEAVPREIILPVRIVDRSN
                     YQPWDRPLEQRQCPRWDEVVGDRRS"
     gene            complement(4828..>5068)
                     /locus_tag="AC16CX_19620"
     CDS             complement(4828..>5068)
                     /locus_tag="AC16CX_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR3014753.1"
                     /translation="ASFVIAPALCGDHPGKRMTYGHSLVVDPWGEVLADGGEAPGVMY
                     ADLDLTQVEKVRGMLPSLAHDRPFAPPKATADERG"
BASE COUNT          852 a         1645 c         1617 g          954 t
ORIGIN      
        1 gggtgcgcga tccggtgaag gtcgcctatc tccgcttttg cgacaagcta cgccgcaaag
       61 gattgccgcg cgcaccagcc gaggggcccg tggactacgc gcgccggata gaaagatcgc
      121 ggcccgagct tcagcccgcg gtcgccgcta tcacccggct ctacatcgcg ctgcgctacg
      181 gcactggcgc cggcgccggc gcattcgacg aactgcagca gcgagtgcga cagttcggag
      241 cctgacttca agtcgaaagg cgaaaggcgg aagtaaaggc tgttagccag tgtcgttcga
      301 cgacttactt tcgacttccg actttcgcct ttcgccttct gcctacgcag ggggcaggag
      361 tttcatcgga tccaccggct tgccgaagcg gcggatttcg aagtgcagct tcacctggtc
      421 ggtatcggtc gagcccatct ccgcgatctt ctggcccctg gcaacgttct gcccctcctt
      481 gaccagcagc tccttgttgt gcgcgtagac gctgaggaag gtgttgttgt gcttgatcac
      541 gatcagcttg ccgaaaccgc ggatgccggt gccgctgaaa atcacgcggc ccggggcgct
      601 cgccagcacc gcctgtcccg gtttgccggc aatgccgatg cccttcaggt tggcgctgtc
      661 attgaacgtg ctgatcaccc tgccgctcgc cggccagccc cattggatgc tctcgttgcc
      721 ttcgtcccct ccggcgggca ggcgcggctc ggtcttgggt gcgggttccg gcttgatccc
      781 cgccagctgg gcataagcct ggtccgagta cggcgcgcgt accgccttgg gctcggcctt
      841 gacgttgtcg cccgcgatcg gcgcgtcgcc cagcggtcgt ccctcgatcc cggggacggt
      901 cttgaacggt gccgccgttg ccatggatgc cggcggcctt aaccacagtt gctgcccggt
      961 gcggatgaca ccggggtcag tgatgtcgtt ccactgcgcg agctcgcggt agtcgagccc
     1021 gtggtcgagc gcgatgctgt agagcgtatc acccctcctg accgtgtaga gctccggccg
     1081 cgcatcggcc ggcttcggcg cgggcttggc ggtgaccgcg ggtttggctg gggcaggcct
     1141 cgcctcaggc gaccgatcca cgacaggggc accgcgcctg gcgacgcaac ccgccagtat
     1201 cgcgacggta agcgcgcacg ccagaacgtt cgtcagggat ggccggatca tctcggattc
     1261 acgttcaagg ttccaggttt gaaaactgaa cttggaacct ggaacttgga acatgggact
     1321 caacccacgc ccggcagcaa gggcacaaag ttgacgggat ccatgcggcg ttcgaccaat
     1381 cccttgtccg tgcgttcgat cacgtaaaga tactgcttgt tcctccccgc catgggcaaa
     1441 atcattctac caccgacggc gagctgctcg accagggccg cgggcgcgct cgccgcagcc
     1501 gccgtcatga cgatcgcgtc gaacggcgcg acctcgggca tgccgacgcg cccgtctcca
     1561 tggcgcaaat ggacgttgga aacgcctgct tcgcgcaggt gcttgcgcgc cttcctgatg
     1621 agggcggcga tgcgctccat cgagtacacc tgtcttgcga ccctggcgag caccgcagtc
     1681 tgatagccgc agccggtgcc gatttccagc gccttggcga gcggccggcc attgcgcgcg
     1741 agctccgtca tgcgtgccac ggtgtggggg ctcgagatgg tctggccgaa cccgagcggc
     1801 agcgacatgt cctcgtaggc gcggctcgcg agggcctcgt caacgaagat gtggcggggc
     1861 aattccccca tgatggccag caccgtctcg tcccggatgc cctgtccgcg cagccgttcg
     1921 accatgcgta gccgcgtgcg ctgcgaggtc atcccgattc ctgggtgctt gctcgatggc
     1981 attacagcgc gcctctcgcc cagagatacc ggggtggaca cgaacaagga tgaaggatga
     2041 tggatgaagg atgacccggt aacgccgttt gcctttcctt gctccttcct ccttccgcct
     2101 tcatccttgc tccttccgcc ttcatccttg ccccgcgagc caggcgctga ccgcctcgaa
     2161 ctgggaatag cgcgtgagat ccacctgcag cggcgtgatc gagacgcggt tgccggcaat
     2221 ggcatggaaa tccgtgcctt cgccggcgtc ctgtgccccg ccggcggcgc cgatccagta
     2281 caccgtctcg ccgcggggcg tggtggattt caccaccggt tccgccttgt gccgccttcc
     2341 gagccgggtg acctggaagc cgcgcagctg cgcgtactcg acatcgggaa cgttgacgtt
     2401 gagcagcacc ggctggccga gcggttgtcg ctggaaccgg ctgacgagct ccagcgccac
     2461 ccgtcccgcg gtccggaaat ggtccccccc ttcagccacc agcgagatcg cgatcgacgg
     2521 tatgcccagc aggaacccct ccgtcgccgc agcgacggtg ccggaataaa tcgtgtcgtc
     2581 acccatgttg gcgccgtgat tgatgccgga gatgacgatg tcaggcagct ccttcagcag
     2641 ccccgtcacc gcgaggtgca cgcagtccgt gggcgtgccg ttgacgtagt agaaccggcc
     2701 ctgcgcccgg cgcacggtga gcggacggtc gagggtaagc gaattgctcg acccgctgcg
     2761 gtcgcgttcc ggcgcgacca ccgttatttc cgcgagcggc gcgagcactt cagcgagcag
     2821 cgtgatgccc ggcgcgaaat agccgtcatc gttgctcagc aggatgcgca caacgatagg
     2881 tgggccgtga acggtgaccg gcaaccggca gcaaggagaa aggaaatggc gatggcgatg
     2941 ggccggtcac gaacaatgtt gagatggcgg ggacagcacg ctatcccacg attgcggcag
     3001 gggcgccggt ctgccgtccg tagatgcgct gaatcttgcc ggtcgcggcg tcgatccgca
     3061 gccgctcgat gttctcgaga tcggtgccgg tgatgcgcag atagcgggtg ttgaccggcg
     3121 tggccgtgga atgcagctcg cgcccgtaat agacccccgc cttgcccggc gtgaggcggt
     3181 agctcggccg cggcttgagc ctggcgtgat cgggatcggt gccgtcgtcg atacggtccc
     3241 attccgtcat ctcggtatat tcagccacct ggccgtagat cgcccaggaa tccccgtggt
     3301 cgtgagggtt ggacttgcgc gcctcgtcgt agcgataggt catgatctcg aacccgagcc
     3361 ggggatcggt gtagatgcgc ttgagccccg tcggttgctg gtcaccgaaa tattccttga
     3421 cgaagccgga gtccaccagc aggcgctcca tgtacgtctt gactttctcc acgtgcttgc
     3481 ggctggagcc cgtcttcaat atcgcgcgcg tgtcgcggca aaaatcctgc aatgaatagc
     3541 ccatgcaagc ccccttttga ctgacgggaa attatagcag gaggagtttg tcgggcagct
     3601 gccggacaaa ctcctgaagg aaacccgcca acatggattc cgagtcgttg ttcatcagga
     3661 gatcggcggt caggatcgcc gatctcctac cacctcgtcc cagcgcgggc attgccgctg
     3721 ctcaagcggc ctgtcccagg gctggtaatt ggaccggtcc acgatcctca ccggcaggat
     3781 gatctcgcgg ggcaccgctt cgccccccag atgccggatg gcggcttcgg tcgcaatgca
     3841 gctgatcttc atcgcgtcga agtcaaccgt agccagcagc ttgccctcct tgatcgcggt
     3901 gatcgcctcg ggaatcgcgt tcacgccgat caggggaatg cgccggccgg ccgcatcgag
     3961 cgccgagagg atgccgagcg acatgacgtc gttcgccgaa aggatgccgt cgatgcgagg
     4021 caatgtggcg agcaggccgg ccatcgcctt tttcgccgtc tcatactggt agtctccggc
     4081 ccgcgtggcg gcgatgcgca cgtcgggcca ctgcttcgct tcatcgcgaa aaccgcgcac
     4141 tcgctcccgg ctcgtcactg agcccggcgt gccctcgatg accaccacgt cacccttccc
     4201 gcccaggtgc cggaacaggt aggccgctat gtcgcgggcg agccggtaat cgtcggagcc
     4261 gacgaagctc acgaacctgc cttcggccag ccggttgaga tagttcacga ccgggatgcc
     4321 ggcggcattg agtccgcgca ccgaggcatc gagcgcagtg gcgtgcaccg ggacgaacac
     4381 gacggcatcg ggatgctccg caaccgcccg ttcgaccagc gcgacctgct cgtccacgtc
     4441 gtccggcttg tccggaacga aatgaacggt gcgtgccccg tagcgcctgg cagtccggtc
     4501 cgcgccgagg cgtgcggcct cgtaagccgg gttcgtgcgg ttcttggtga acaccgcgat
     4561 cgtttctccg gacatctaca agaccttgta caaaacggta acgaaaccgc cgatgaacgc
     4621 agataaacgc aaatacatca ggaatagctg ctgtattgcc tttgcgctat atcggcgttc
     4681 actggggatt tctgtgtcca ggttccattc ttgattcccg agagaagctc gtgcatgtat
     4741 atcaactgca agagacgggt cacggatgcc ccgagataaa cgcggatgac gttaccgctt
     4801 gctgtccggt caagtaatta tcagcgttta tccgcgttca tctgcggttg ctttgggcgg
     4861 ggcgaacggc cggtcgtggg cgagcgaagg cagcatgccg cgcactttct ccacctgcgt
     4921 gagatcgaga tcggcgtaca tcacgcccgg cgcctcgccc ccgtcggcga gcacttcgcc
     4981 ccacgggtcc accaccagcg aatggccgta ggtcatgcgc ttgcctggat ggtccccgca
     5041 cagggccggc gcgatgacaa agctcgcg
//
LOCUS       DBNGWZ010000626         4110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_984593, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000626 DBNGWZ010000000
VERSION     DBNGWZ010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_984593"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16CX_19625"
     CDS             complement(<1..452)
                     /locus_tag="AC16CX_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HIR3014754.1"
                     /translation="MTVDVGRTFLSVVALTVAMSWHGDTRAACPSHERINTLLRFMEA
                     REPVRGLRADLSMQDAECGRRRLVEKLESSENRIVGYKAGLTNKAVQERFGVASPVRG
                     VLLEKMLLEDGAVVPADFGARPVFEADLLVVVKDAAIHKAKTHLDVLRS"
     gene            648..2660
                     /locus_tag="AC16CX_19630"
     CDS             648..2660
                     /locus_tag="AC16CX_19630"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3014755.1"
                     /translation="MILYALTIFTSAFLLFLVQPIMAKQILPWFGGSAAVWTTCLVFF
                     QVLLLAGYAYSDWTARKLPARRQVALHLVLLAASLASLPIIPDAGWKPAGDEDPAWRI
                     LGLLAVTIGLPYFLLSTTGPLVQAWFARTFPAGTVYRLFALSNFGSLLALISYPFAFE
                     PWITTLDQSRGWSVAYVAFVVLCAASAVYSVLARAQGTGSAEAVTVPNDGPPPRAGDY
                     AAWLLLSAMGAFMLLAVTNHITHDVASVPFLWILPLMLYLVTFILCFEGRGWYQRVIF
                     LGPLAVIVGAMAWALYAERGIMEIKEAIPLNLVGLFVMCMFFHGELAARKPAPRYLTS
                     FYLMVSLGGALGGLAVGIVAVKVFNTYYEFGIGLVLTLAIAAYLTRLMHPAVPMLALA
                     AVGFTGFYIYQYIGSLTRDAHVMTRNFYGTLRVKDVGAGEDAVRRLMHGIIMHGEQYR
                     AEDRRNEPTTYYGATSGIGRAIKAFDSSPLRVGVVGLGTGTLAAYGRQDDVYRFYEIN
                     PQVVEIARREFSFLGDSDAAVETVLGDARLAMEREPPQNYDLLVIDAFSSDSIPVHLI
                     TREAMAVYLRHMKPGGVIAFHVTNRFLDLAPVVKRIADEQRLHTALISDDAEDSELAR
                     TDWVLVTRNKAFLERPDIAEFADDIEDIPGLRVWTDDFNNLFQILK"
     gene            2704..4089
                     /locus_tag="AC16CX_19635"
     CDS             2704..4089
                     /locus_tag="AC16CX_19635"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR3014756.1"
                     /translation="MRIVPMVRKLKFLICSALAAIYCAGSMAAAQVHTDARGLPTLAP
                     LVNEVTPAVVNISVVTRSPLEDNPLFRDPFFRRFFNLPEKPQRQEQAAGSGVIVDAGR
                     GYVLTNHHVIKDAEQAIVTLKDRRQFRAKLVGTDPGTDIAVLQIEARDLSAIRIGDSD
                     TLQVGDYVIAIGNPFGIGQTVTSGIVSALGRSGLSPEGYEDFIQTDASINPGNSGGAL
                     VNLRGELVGINTAIIGPAGGNVGIGFAVPSNMARGVMNQIVRFGEVKRGRLGIEMADL
                     TPAVAKKLGVSAQEGAVVAVVQPGSPAEKAGLHERDVIVALNGRPIRTAAELRARLGL
                     TPVGEEIELRVLRAAGTRSIRTRIAAPQEPTAAEGQAIAQLPGMRVVEIERGSPLFQR
                     LRGGGLVVAAVEQGSRAFQAGFRPADIIYAVNRRRVQTVAEFQAAVRGAQGGYAVSLL
                     RGDFNVTIIVR"
BASE COUNT          642 a         1410 c         1331 g          727 t
ORIGIN      
        1 gagcgcagca cgtcgaggtg cgtcttcgct ttgtggatcg ccgcgtcctt caccacgacc
       61 agcaggtccg cttcgaatac cgggcgcgcc ccgaaatccg ccggcacgac cgcgccgtct
      121 tccagcagca ttttctcgag cagcacgccg cgcaccgggc tcgcgacgcc aaagcgctcc
      181 tgcaccgcct tgttggtgag gccggccttg tagccgacga tgcggttctc gctcgactcc
      241 agcttctcga ccaggcgccg gcgaccgcat tccgcgtcct gcatcgagag gtccgctcgc
      301 agcccgcgca ccggctccct cgcttccatg aagcgaagca gcgtgttgat ccgctcgtgg
      361 ctcgggcacg cagcgcgggt gtcgccgtgc cacgacattg cgactgtcaa cgcaactacc
      421 gacaggaacg ttctcccaac atcaacggtc atcagtcgtc accatgatcc ggtctggcat
      481 gagtcaagga cgagggatga aggatgaggg atgaccgggt tattccgccc gcactgcggg
      541 ggtcatcctt ccgccttccg ccttcatcct tcaggcgtgc gcgctgcgca tcaggggcgc
      601 acgccgaagc gccggtataa tacgcgcttt tcgcacgcgc ccgactgatg attctctacg
      661 ccctcacgat cttcacgagc gcgttcctgc tgttcctggt gcagccgatc atggcgaagc
      721 agatcctgcc gtggttcggc ggctccgccg cggtgtggac cacctgcctc gtgttcttcc
      781 aggtgctgct cctcgccggc tacgcgtact cggactggac cgcgcgcaag ctccccgcgc
      841 gcaggcaggt cgcgctccac ctcgtgctgc tcgccgcgag cctcgcatcg ctgcccatca
      901 ttcccgatgc cggctggaag cccgccggcg acgaggatcc ggcctggcgc atcctgggac
      961 tgctcgccgt caccatcggg ctgccctact tcctgctctc gaccaccggg ccgctggtgc
     1021 aggcctggtt cgcgcgcact tttcccgccg gcacggtgta ccgcctgttt gcgctgtcca
     1081 acttcggctc gctgctcgcg ctgatctcct acccgttcgc attcgagccg tggatcacca
     1141 cgctcgatca gtcccggggc tggagcgtgg cgtacgtcgc gttcgtcgtg ttgtgcgcgg
     1201 cttccgccgt ctacagcgtg ctcgcgcgcg cgcagggcac gggcagcgcg gaagcggtca
     1261 cggttccgaa tgacggcccg ccgccccggg ccggggacta tgcggcgtgg ttgctgcttt
     1321 cggcaatggg cgctttcatg ctgctcgccg tgaccaacca catcacccac gacgtggcct
     1381 cggtgccgtt cctgtggatc ctgccgctca tgctctatct cgtgaccttc atactctgct
     1441 tcgagggccg cggctggtac cagcgcgtca tcttcctcgg gccgctcgcc gtgatcgtcg
     1501 gcgcgatggc ctgggcgctg tacgcggagc gcggcatcat ggagatcaag gaggcgatcc
     1561 cgctgaacct cgtcgggctg ttcgtgatgt gcatgttctt ccacggcgag ctcgccgcca
     1621 ggaagcccgc gccgcgctac ctgacgagct tctacctgat ggtgtcgctg ggcggcgcgc
     1681 tcgggggcct cgccgtggga atagtcgccg tgaaggtctt caacacctac tacgagttcg
     1741 gcatcgggct cgtgctgacg ctggcaatcg cggcgtacct cacgcgcctc atgcacccgg
     1801 cggtgccgat gctggcgctc gccgccgtcg gtttcaccgg cttctacatc taccagtaca
     1861 tcgggtcgct cacccgggac gcgcacgtca tgacacgcaa cttctacggt accttgcgcg
     1921 tgaaggacgt cggcgccggc gaggacgcgg tgcggcgcct gatgcacggc atcatcatgc
     1981 atggcgagca gtaccgcgcc gaagatcggc gcaacgagcc gaccacctac tacggcgcga
     2041 cctccggcat cgggcgcgcg atcaaggcgt tcgattcatc cccgctgcga gtcggcgtgg
     2101 tggggcttgg caccggtacg ctcgccgcct acggccgcca agacgacgtt taccggttct
     2161 atgagatcaa cccgcaagtg gtggaaatcg cgcgccggga attcagcttc ctcggcgaca
     2221 gcgacgcggc ggtcgaaacc gtcctggggg acgcgcgcct tgcaatggag cgcgagccgc
     2281 cgcagaacta cgatctgctg gtgatcgacg cattctcgag cgattcgatc cccgtgcacc
     2341 tcatcacgcg cgaggcgatg gccgtgtatc tccggcacat gaagcccggt ggcgtgatcg
     2401 cattccacgt caccaaccgc tttctggacc tcgcccccgt ggtcaagcgc atcgccgacg
     2461 agcagcgact gcacaccgcg ctgatctcgg acgacgcgga ggacagcgaa ctcgcacgca
     2521 ccgactgggt gctggtcacg cgcaacaagg cctttctgga gcgtcccgac atcgcggagt
     2581 tcgccgacga catcgaggac atcccggggt tgcgggtgtg gacggatgat ttcaacaatc
     2641 tcttccagat actgaaatag caccgtcttc gccggcgcta tttcagtaat agaggacaat
     2701 aaaatgagaa tcgttcctat ggtccgtaaa ttgaaattcc tgatctgcag cgcgcttgcc
     2761 gcaatttatt gcgctggctc catggcggcg gcgcaagtgc ataccgatgc gcgcgggctg
     2821 ccgacgctgg cgccgctggt caacgaggtg acgccggcgg tggtgaacat ctcggtcgtc
     2881 acgcgctccc cgctcgagga caatcccctt ttccgcgatc ccttcttccg gcgcttcttc
     2941 aacctgcccg agaagccgca gcgacaggag caggccgccg gctcgggcgt gatcgtggac
     3001 gccgggcgcg gctacgtgct caccaaccat cacgtgatca aggatgccga gcaggcgatc
     3061 gtcacgctca aggaccgccg ccagttccgc gccaagctcg tcggcaccga tcccgggacc
     3121 gacattgcgg tgctccagat cgaggcgcgg gatctctccg cgatccggat cggcgactcc
     3181 gacacactgc aggtcggcga ttacgtgatc gcgatcggca atccgttcgg catcgggcag
     3241 accgtcacct ccggcatcgt cagcgcgctg ggaaggagcg gcctctcccc cgagggctac
     3301 gaggatttca tccagaccga cgcttcgatc aaccccggca attcgggcgg cgcgctcgtc
     3361 aacctgcgcg gcgagctggt cggcatcaac accgcgatca tcggccccgc aggcggcaac
     3421 gtcggtatcg gctttgcggt gccgtccaac atggcgcgag gggtgatgaa ccagatcgtg
     3481 cgcttcggcg aggtcaaacg cgggcgcctg gggatcgaga tggcggatct cacgcccgcc
     3541 gtcgcgaaga agctcggcgt cagcgcgcag gaaggggcgg tggtcgccgt cgtccagccc
     3601 ggctcgccgg cggagaaagc ggggctgcac gagcgcgacg tgatcgtcgc gttgaacggc
     3661 cgcccgatcc gcacggccgc cgagctgcgc gcgcgactgg ggctgacgcc ggtgggcgag
     3721 gagatcgagc tgcgggtgct gcgcgcagcc gggacgcgca gcatccggac gcgcatcgcc
     3781 gcgccgcagg aaccgaccgc cgccgaaggc caggccatcg cacagttgcc gggcatgaga
     3841 gtggtcgaga tcgagcgcgg cagcccgctt tttcagcgcc tgaggggcgg cgggctggtg
     3901 gtggcggcgg tggaacaggg cagccgcgcc ttccaggccg ggttccggcc ggccgacatc
     3961 atctacgccg tcaaccgccg gcgcgtgcag acggtcgccg agttccaggc cgcggtgcgc
     4021 ggcgcgcagg gcggctatgc ggtgtcgctg ctgcgcggcg acttcaacgt tacgattatc
     4081 gtccgctaat tgccgttaaa tccggaccgc
//
LOCUS       DBNGWZ010000627         6060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3126852, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000627 DBNGWZ010000000
VERSION     DBNGWZ010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3126852"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /locus_tag="AC16CX_19640"
     CDS             complement(<1..1648)
                     /locus_tag="AC16CX_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014757.1"
                     /translation="MTTLLPECRQTRWVAGVSLAVSAVIALALAQQRAGVGPFEIPVY
                     RHLILFQDYYVLLPFIGILAAALFAPVRTLGVHAARWCGRHVWTVAGVTTAALAIGTH
                     SVYHVQPLSLDEYAVVFQSKIFLEGRLTGQFPPALLDWLIPKFFQGRFLKISPETGAV
                     VSVYWPGFSLLLVPFTALGASWLLNPLIGGVTVLVMHRIALTLFGSVESAGYVVLLTL
                     ASPAITINALSYYSMPAHLLANALFMLLLLRPVPIRALAAGLVGSVALVLHNPVPHLL
                     FAVPWVVWLAFQPGRVRLLGALAAGYLPVCLVLGWGWAIFLQSIGSPMAAGDLATPTG
                     AAGTLLYRLGTVLGWTSSTGVSTHVLDLCKLWLWAVPALLTVAALGAWRLPESRGVWL
                     VMASCALLTYFGYFVVRFDQGHGWGFRYFHSAWLVLPLFAVRAIALPGRGHLAGYLAG
                     CALLSLVVLTGFRALQVEQFMARHLSQLPVAANGEARVRIVNPTNGYYAWDLIQNDPF
                     LRGTVIFGSRGAQADAALMAARFPQYERLSEDRRGSVWGIA"
     gene            complement(1645..2709)
                     /locus_tag="AC16CX_19645"
     CDS             complement(1645..2709)
                     /locus_tag="AC16CX_19645"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR3014758.1"
                     /translation="MLHERVRAARNSCQLLVLVLALGSCASLPNSSSRAGERSYTNPV
                     LERDFPDPTVLRAPDGWFYAYATQSESGNGMINVQIARSRDLVRWEHLGDALPEKPYW
                     AAAKQNFWAPHVMHDPAQGKYFMYYSGEPNETTGKCLAVATSEAPGGPFVDSGSPLLC
                     GESFEHIDPMAFDDPQTGKRLLYWGSASRPIKVRELAPDRQRFLPGSAPKEILSPDEQ
                     SPNSSLIEGAWVTFRNGTYYLFYSGDRCCAPDPSYAIMVARSNNAFGPFERYDSPDGS
                     SIILRENEFWRAPGHNSVVKDADDTDWILYHAFPRDATQGPGRRRAMLLDRIVYQDGW
                     PRIAGDQPSVTPQNSPVVRP"
     gene            2782..3705
                     /locus_tag="AC16CX_19650"
     CDS             2782..3705
                     /locus_tag="AC16CX_19650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3014759.1"
                     /translation="MNESAGGGHALRPGRAEGGWRLRARESDPPPLVSIITSTLDAAE
                     HLPHTLRSVAAQKGKLSEWIIVDGGSSDGTVELLRGHDDLIARWLSEPDAGIYDAWNK
                     ACVLARGQWLLFLGAGDEFAASDTLAQFSEHLAGAHPAHDLVYGKLIYISQTGRRDLE
                     EVGLPWTELEGRWELGRPALPPHPATFHHRSLFAGGRAFDTHFRLAGDSHFLLRYALR
                     NAPLFVPLAVCRSLVGGVSMNLGTAVALAREIRDMNRELALVPPLRHRLSENLLLAAK
                     VVAGRMPSPIGRRIADVYRRLIGRPKRWSVR"
     gene            3724..4983
                     /locus_tag="AC16CX_19655"
     CDS             3724..4983
                     /locus_tag="AC16CX_19655"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR3014760.1"
                     /translation="MKRYVVNSIWLFAEQALRLIAGFLVGVYVARYLGPTQFGTLSYS
                     LAFVALFGAIARLGLDAVVIRELIANPERRSALLGTAFWLKVAGALVALLLIAVSIGL
                     ASAPENVRVYVIVIGCGLAMQAFDVVDLDFQARSLPKYAAICRMYQLAISSSVKLVLV
                     ATQAELWAFVAVSLFDQLVLAAAFVIAMRRTEGVARFFRRFDTEAARQLFSAAAPLAV
                     AALMVAVYMRVDQLLVMELLGVRAVGIYAAASNLTEALYIVPTVVASSFFPAILYARP
                     SATLYQARLSSLYKATLGFGVVSALLVSAGSDAIVRLLYGPQFAEAAPVLAVHVWTLI
                     VVCLSAVLGKWLVAEALQSLVPRMTLVAMIVNIAMILVLVPAFGLVGAAAAACVAQIV
                     PALLFLSLDARLRAHLRGALLPAANRP"
     gene            4980..5975
                     /locus_tag="AC16CX_19660"
     CDS             4980..5975
                     /locus_tag="AC16CX_19660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014761.1"
                     /translation="MKAAPRPVDPESPATPAYRCCAAMQPMEIGRVYDDRYGYPDPAR
                     AVHCVACGHTQLVDTPPAEALGPMYSTFYPRSAFDPADYHAYVPPRGRIRGWLDGARA
                     VAGLWVRPCSRVLDIGCGYGEMLGYLEGIGCEVEATEIDQNIAELARRRGFKVRIGPF
                     DERNHPQGHFDYVLLNQVIEHFPDPGALLHRIRGVLAPGGTVVITTPNGGGILRRVFG
                     KRWIHWHAPYHVQIFTRRSMLNLARTHGYDVRLLRTLSPSSWLLYQWCHGIGYPEPGH
                     AHPFWSERVPKSLVQRVSYRLIHVATHASRINHLVMRCLDGAGIGDNLLVLLSKP"
BASE COUNT         1018 a         1941 c         2013 g         1088 t
ORIGIN      
        1 gcgcgatgcc ccacaccgat ccgcgccggt cctcggacag ccgctcgtat tgcggaaagc
       61 gtgcggccat cagcgcggcg tccgcctggg cgccgcgcga gccgaagatg accgtgccgc
      121 gcaggaacgg atcgttctgg atcagatccc aggcgtagta gccgttcgtc gggttgacga
      181 tgcgcactct cgcctcgccg ttggcggcga ccggcagctg cgacaggtgg cgtgccatga
      241 actgctcgac ctgcagcgcg cggaagcccg tgagaacgac gaggctcagc agcgcgcatc
      301 cggcaaggta accggcgagg tgcccgcgcc ccggcagggc gatcgccctc acggcgaaca
      361 gcggcaggac cagccaagcg gaatggaaat agcggaatcc ccagccgtgt ccctgatcga
      421 accgcacgac aaagtagccg aaatacgtca gcaaggcaca gctcgccatg accagccaca
      481 cgccgcgact ctcgggcagc cgccacgccc cgagcgcggc gaccgtcagc agggcgggga
      541 cagcccagag ccacagcttg cagagatcga gaacgtgcgt gctgacaccg gtcgaagaag
      601 tccagcccag cacggtgccc agccggtaga gcaaggtccc ggccgcgccc gttggcgtcg
      661 cgagatcgcc cgcagccatt gggcttccga tgctttgaag gaaaatggcc catccccatc
      721 cgagaaccag acacacgggc aggtagccgg cggcgagggc accgaggagc ctgacacgac
      781 cgggctgaaa ggcgagccac acgacccacg gtacagcgaa cagcaggtgc ggcaccgggt
      841 tgtgcagaac tagggcgacc gagcccacca gtccggcggc gagcgcccgg atgggcaccg
      901 gccgcagcag gaggagcatg aaaagcgcgt tcgcgagcag gtgcgcaggc atcgagtagt
      961 acgacagcgc attgatggtt attgccggcg aagcgagtgt gagaaggacg acgtagccgg
     1021 cgctctcaac gctgccgaac agcgtgagcg cgatccggtg catgacgaga accgtcacgc
     1081 ccccgataag gggattgagc agccacgacg ccccgagcgc ggtgaaggga accaggagca
     1141 gcgagaatcc gggccagtac acggacacga cggcgccggt ctcgggggag atcttgagaa
     1201 atctcccttg gaagaacttc gggatcagcc agtcgagcag cgccggtggg aactgcccgg
     1261 tcaatcggcc ctcgaggaat atcttgctct ggaaaacgac ggcgtattcg tccagggaaa
     1321 gcggctggac gtggtaaacc gagtgcgtac ctatcgcgag tgctgcggtg gtgacgccgg
     1381 ctaccgtcca gacatgccga ccgcaccagc gggcagcatg gactcccaac gtcctgaccg
     1441 gtgcaaaaag cgccgccgcg aggatgccga tgaatggcag gagcacgtag taatcctgga
     1501 aaaggatgag atggcggtag accggaattt cgaacggacc gacgcctgcc ctctgctgcg
     1561 cgagcgcgag cgcgataacc gcagagacgg caagactgac gcccgccacc cagcgggtct
     1621 gtctgcactc cggtagcagg gtcgtcacgg ccgcaccact ggggaattct gaggcgtcac
     1681 ggaaggctga tcccctgcaa tgcgtggcca gccgtcctga tagacgatcc ggtcgaggag
     1741 catggcacgg cgcctgcccg gcccctgcgt cgcgtcccgc ggaaaggcgt ggtagaggat
     1801 ccaatcggtg tcatccgcgt ctttcacgac gctgttgtgg cccggcgccc gccaaaattc
     1861 gttttcgcga agaataatgc tgctgccatc cggcgagtca taccgctcaa atggtccgaa
     1921 ggcgttattc gagcgggcaa ccatgatggc gtagcttgga tcgggggcgc aacagcgatc
     1981 gcccgagtag aaaaggtagt acgttccgtt ccgaaaagtc acccaggcgc cctcgatcag
     2041 cgagctgttc gggctttgct catccgggga aaggatctct ttgggcgcgc tgcccggcag
     2101 gaagcgttgc cggtccggcg ccagctcccg caccttgatg ggtctggagg ccgagcccca
     2161 gtaaagcagg cgtttccccg tctgcggatc gtcgaacgcc atcggatcga tgtgctcgaa
     2221 gctctcgccg cacagcaacg gactgcccga gtcaacaaac ggccctcccg gggcctccga
     2281 agtcgccacc gccaggcact tgccggtcgt ctcgttcggt tcacccgagt agtacatgaa
     2341 gtacttccct tgcgccggat cgtgcatcac gtgcggcgcc cagaagttct gcttggcggc
     2401 cgcccaatac ggcttctcgg gcagcgcgtc gcccaggtgc tcccatcgca ccagatcgcg
     2461 cgagcgtgcg atctggacgt tgatcatccc gttgccggac tcgctctgcg tggcatacgc
     2521 gtagaaccag ccatcgggcg cgcgcagcac agtgggatcg gggaaatccc gctccagaac
     2581 agggttcgta taagagcgtt cgccagcgcg ggagctactg ttggggaggg acgcgcacga
     2641 gccgagcgcc agcacgagaa cgagcagttg acaggaattc cgcgcggcgc ggacgcgctc
     2701 atgcagcatg atcgcgcagt gtacaataac gacgtcattg cattatcgtg cgttctgcac
     2761 gaagcatccg gcaggcggat tatgaacgaa tcggcgggag gcggccacgc gctgcggccg
     2821 gggcgggccg agggcggatg gcgcctgaga gcccgcgagt cggatccgcc gccgctcgtg
     2881 tcgatcatca cctcgacgct cgacgccgcg gagcatctgc cccatacgct caggtcggtc
     2941 gcggcgcaga agggaaagct ctcggaatgg atcatcgtcg acggcggctc gagcgacggc
     3001 accgtcgagc tgcttcgtgg tcacgacgat ctgatcgcgc gctggctgag cgagcccgat
     3061 gcgggcatct acgacgcctg gaacaaggcg tgtgtgctgg cccgggggca gtggctgctg
     3121 ttcctggggg cgggcgacga attcgctgct tccgatacgc tggcgcaatt ctcggaacat
     3181 cttgccggtg cacatcccgc ccatgatctg gtctacggca agctgatcta catcagccag
     3241 acggggaggc gggatctcga ggaggtcgga ttgccgtgga cggaactgga agggcgctgg
     3301 gagttgggcc ggccggccct ccctccgcac ccggcgacct ttcaccaccg ctccctgttt
     3361 gccggcgggc gcgccttcga tacgcacttt cgtctcgccg gagactctca cttccttttg
     3421 cggtatgcac tgcgcaatgc gccgctgttc gttcccctgg ctgtctgccg gagcctcgtg
     3481 gggggcgtgt ccatgaatct gggaaccgcg gtcgccttgg cgcgcgagat ccgcgacatg
     3541 aaccgggaac tggcgctcgt tccgccgctt cgccatcggc tgtccgagaa cctgttgctc
     3601 gctgcgaagg tggtggccgg gcgcatgcct tcaccgatcg ggcgccggat tgccgatgtt
     3661 taccggcgcc tgatcggacg ccccaaacga tggagcgtgc gctaggtgat gccaggtggc
     3721 gagttgaagc gctacgtggt caattcgatc tggcttttcg cggagcaggc gctgcggctg
     3781 atcgccggtt tcttggtcgg cgtctacgtg gcccgctact tgggaccgac gcagttcggc
     3841 accctgagtt actcgctggc gttcgtcgcg ctcttcggcg ccattgcccg gctcggcttg
     3901 gatgccgtcg tgatccggga gctcatcgcc aaccccgaac ggcggtcggc gctgctcggc
     3961 accgcattct ggctgaaggt cgctggagcg ctcgtcgccc tgctcttgat cgccgtgtcg
     4021 attggactgg ccagcgctcc ggagaatgtc cgcgtttacg tgatcgtcat cggctgcggt
     4081 ctcgccatgc aggctttcga cgtcgtcgat ctcgatttcc aggcgcggtc gcttccgaaa
     4141 tatgcggcga tctgccggat gtatcagctt gcgatctcgt cgtcggtaaa gctggtgctg
     4201 gtcgcgacgc aggccgagct gtgggccttc gtcgccgtct cgctgttcga ccagcttgtt
     4261 cttgccgcgg ccttcgtcat cgccatgcgc cgcacggaag gcgtcgcgcg cttctttcgg
     4321 cgttttgata cggaggcggc gcgccaattg ttctccgcag cagcgcccct tgctgtcgcg
     4381 gccctcatgg tggccgttta catgcgcgtc gaccaactcc tcgtcatgga gctgttgggg
     4441 gtgcgcgccg tcggcatcta tgcggcggcc tcgaatctga ccgaggcgct ctacatcgtg
     4501 ccgacggttg tcgcaagctc gttctttcca gcgatccttt acgcgcgccc gtccgcgacg
     4561 ttgtaccagg cacgcctgtc ctcgctctac aaagcgaccc tcgggttcgg cgtggtgtct
     4621 gcgctgctgg tgtcggccgg ttccgacgcg atcgtgcggt tgctatacgg gccgcagttc
     4681 gcggaggcgg cgccggtcct cgcggtccac gtctggaccc tgatagtggt ttgcctttca
     4741 gccgtcctgg gcaagtggct ggtcgccgag gcgcttcaat cgctcgtgcc gcgcatgacc
     4801 ttggtcgcaa tgatcgtgaa catcgcgatg attctggtgc tggttccggc attcgggctg
     4861 gtcggtgcgg cggctgcggc gtgtgtcgct caaatcgttc cggcgctgct gttcttgtcc
     4921 ctggacgccc gcctgcgcgc ccatttgcgg ggcgcattgc tgccggcggc gaacaggcca
     4981 tgaaggccgc gccccgcccg gtcgatccgg agtcgccggc cacgccggcg tatcgctgct
     5041 gcgcggcgat gcagccgatg gaaatcggaa gggtatatga cgaccgctac gggtatccgg
     5101 atccggcgcg tgccgtccac tgcgttgcgt gcggccacac gcaactggtc gatacgcctc
     5161 cggccgaggc actgggcccg atgtattcga cattctatcc gcgatcggcg ttcgatccgg
     5221 cggattacca tgcctacgtg ccgccacggg ggcgcattcg cggctggctc gatggcgcgc
     5281 gtgcggttgc cggcctctgg gtgcgaccgt gctcgcgcgt cctggatatc ggctgcggct
     5341 atggcgagat gctgggctat ctcgagggaa tcggctgcga ggtcgaggcg accgagatcg
     5401 accagaacat agccgagctg gcgcgcaggc gcgggttcaa ggtcaggatc ggccctttcg
     5461 acgagcgcaa tcatccgcaa gggcacttcg actatgtgct tctcaatcag gtcatcgagc
     5521 atttccctga tcccggcgcg ctgctgcaca ggattcgcgg cgtgctggcg ccgggcggga
     5581 cggtggtcat cacgacaccg aacggcggcg gcatcctccg ccgcgtcttt ggcaagcgtt
     5641 ggatccactg gcacgcgccg tatcacgttc agatattcac gcgccgatcc atgctcaatc
     5701 tcgcccggac gcacggttac gacgtgcgct tgctcagaac gctttccccg tcgtcctggc
     5761 tcctctatca atggtgtcac gggatcgggt atcccgagcc gggtcatgcg catccgttct
     5821 ggtccgagcg ggttccgaag tcgctcgtgc aacgcgtgtc gtaccgcttg attcacgtcg
     5881 caacgcacgc ttccaggatc aaccacctcg tgatgcggtg tcttgatggc gccggcatcg
     5941 gcgacaacct gcttgtgctg ctgtcgaagc cctgatgctt tacgtcctcc tgcccgtgca
     6001 taaccggctc gccacgacgc agcgttgcgt ccgctgcctg ctggcccaga cccatgacga
//
LOCUS       DBNGWZ010000628         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2246782, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000628 DBNGWZ010000000
VERSION     DBNGWZ010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2246782"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC16CX_19665"
     CDS             <1..602
                     /locus_tag="AC16CX_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014762.1"
                     /translation="WYWFSAMTFTFFIAVFWFYWSGLELGVPARLHEHLHRIRPGYAP
                     GFRWLPFVLGVAYSLFWFAVLASFRKSPMRPVAVWGTGITVMWALMATLFLGWVDNQK
                     TYRSMIASLQLVLPKQYDCLSSRDLGEPQRAMLHYFAGIITYREEVPERRRACELLLV
                     QGKPLDEIAPPGPWQKIWEGARPGDKDERYRLYQRTLAR"
     gene            complement(684..1190)
                     /locus_tag="AC16CX_19670"
     CDS             complement(684..1190)
                     /locus_tag="AC16CX_19670"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HIR3014763.1"
                     /translation="MARHYLERLIFSFQDREFGMRVPRSLLACLGAASFAWTPVAMGA
                     SLFSGLSCPSKIEVEQRVTAVPRGWESAQVTVTASLASVTFFDGPPAERASLKYDSEE
                     RQKRNWVARWNLLPNTRGYWISCGYENTATVISRRLPDDVRSCAVTYERRKRGAAGLP
                     GITDISCK"
     gene            1366..>2304
                     /locus_tag="AC16CX_19675"
     CDS             1366..>2304
                     /locus_tag="AC16CX_19675"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR3014764.1"
                     /translation="MASGREVVVLSGVRTAIGDYGGGLKDLAPTELSAQVIREAVKRA
                     KVDAKEVGACVIGNVIHTEAKDMYFSRVAALKGGLPEDSTALTVNRLCGSGMQAIVSA
                     SQMIMLGDTDVAVGGGAESMSRGAYILPSLRWGQRMSDGNVIDMMVGALTDPFDAVHM
                     GLTAEAIAEKWKISREQQDEFSVESHRRAINAIEKGYFKDQILPIELKTRKGTTVFDR
                     DEHPRADASLEGMAKLRAAFKKDGSVTAGNASGINDGAAAVVLMERAAAEKKGLKPMA
                     RLVSYGIAGVDPKIMGIGPVPASKKALDKAGLKVEQM"
BASE COUNT          442 a          732 c          736 g          394 t
ORIGIN      
        1 cctggtattg gttcagcgcc atgacgttca ccttcttcat agcggtgttc tggttctact
       61 ggagcgggct cgagctcggt gtgccggcgc gcctgcacga gcacctgcac cgcatccggc
      121 ccggttacgc accgggcttc cgctggctgc cgttcgtgct gggcgtcgcc tactctctgt
      181 tctggttcgc ggtgctggcg tcgttccgga aaagtccgat gcgcccggtc gctgtgtggg
      241 gcaccggtat caccgtcatg tgggcgttga tggccacgct ctttctcggc tgggtcgata
      301 accaaaagac ctaccgctcg atgatcgcct cgttgcaact ggtgctgcct aaacagtatg
      361 actgcctgtc gagccgcgat ctcggcgagc cgcagcgcgc gatgctgcat tacttcgcgg
      421 gcatcatcac ctaccgcgag gaagtgcccg agcggcgccg cgcctgcgaa ctgctgctgg
      481 tgcagggcaa accgctggat gaaatcgcgc cgcccgggcc gtggcagaag atctgggaag
      541 gcgcgcggcc gggggacaag gacgagcgct acaggcttta tcagcgcact ctcgcacgct
      601 gataaagcct ggaaggatga aggcggaagg cagaaggatg acctggttct aactccagca
      661 ctgcgggggt catccttcat ccctcacttg caggaaatgt ccgtgatccc cggcagcccc
      721 gccgcgccac gcttcctgcg ctcgtaggtg acggcgcagc ttctgacgtc gtccggcagg
      781 cgtcgcgaga tgacggtggc cgtgttctca tagccgcacg aaatccagta gccgcgcgtg
      841 ttgggtagca ggttccagcg cgcaacccaa tttcgcttct gccgttcttc gctgtcgtat
      901 ttcagcgatg cccgctccgc cggcgggccg tcaaaaaatg tgaccgaggc gagcgatgca
      961 gtgacggtaa cctgcgcaga ttcccaaccg cgcggcaccg cggtgaccct ctgctccact
     1021 tcgattttcg atggacaact caacccggaa aatagtgaag cgcccatcgc aaccggcgtc
     1081 catgcgaatg atgcggcccc aaggcatgca agcagcgagc gcggcactct cataccaaat
     1141 tcccgatcct ggaaggagaa gatcagccgt tcaagatagt ggcgcgccaa gcgctgccgt
     1201 gtccacccac cactcacgac tcaccactca ccctgcggat gctgtattgc aacaatttct
     1261 cgacagcccc cctgatccgg cgtaaactcc ccgctctccc ccgaagttaa gtcacttgca
     1321 atcaagtcgt taatccattt ccaatcaaat agtaaggagc aggacatggc aagcggacgc
     1381 gaagtggtgg tgctctccgg cgtgcggacg gcgatcggcg attacggcgg cgggctgaag
     1441 gacctcgcgc cgaccgagct gtcggcgcaa gtgatacgcg aagcggtcaa gcgcgccaag
     1501 gtggatgcga aggaagtcgg cgcgtgcgtg atcggcaacg tgatccacac cgaggcgaag
     1561 gacatgtact tctcgcgcgt cgcggcgctc aaaggcgggc tgccggagga ttccaccgcg
     1621 ttgaccgtca accgcctgtg cggcagcggc atgcaggcga tcgtctccgc gtcgcagatg
     1681 atcatgctgg gcgataccga cgtcgcggtc ggcggcggcg ccgagtccat gagccgtggc
     1741 gcctacatac tgccgagcct gcgctggggc cagcgcatga gcgacggcaa cgtgatcgac
     1801 atgatggtgg gcgcgctcac cgacccgttc gacgccgtgc acatgggcct caccgccgag
     1861 gccatcgcgg agaaatggaa gatctcgcgc gagcagcagg acgagttctc ggtggagagc
     1921 caccgccgcg cgatcaacgc gatcgagaag ggctacttca aggaccagat cctgccgatc
     1981 gagctgaaga cccgcaaggg cacgaccgtg ttcgaccgcg acgagcaccc gcgcgcggac
     2041 gcgagcctgg aaggcatggc gaagctgcgc gccgcgttca agaaggacgg ctccgtcacc
     2101 gccggcaatg cctccggcat caacgacggc gcagcggcag tcgtgctgat ggaacgggcc
     2161 gccgccgaga agaaaggcct caagccgatg gcgcgccttg tctcctacgg catcgccggc
     2221 gtggatccca agatcatggg catcggcccg gtgcccgcct cgaaaaaagc gctcgacaag
     2281 gcggggctga aggtggaaca gatg
//
LOCUS       DBNGWZ010000629         4918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3127763, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000629 DBNGWZ010000000
VERSION     DBNGWZ010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3127763"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1048)
                     /locus_tag="AC16CX_19680"
     CDS             complement(305..1048)
                     /locus_tag="AC16CX_19680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR3014765.1"
                     /translation="MNATDPLKNIRVVLCKPAHPGNIGAAARALKTMGLGRLCLVAPV
                     RFPDPQAEWLASGATDVLRSARVCASLDEALAGSAFTVACTARPREIAVPTVSAREAA
                     ARIIEVARAQPVALVFGNETFGLTAAEVNKCQLLATIPANPEYTSLNLAAAVQVFAYE
                     LRLAAEGGAMNGGRPRTLATHEEVERFYEHLERSMRETGFLNPEHPKKLMPRLRRLFG
                     RAQLEKEEVNILRGIIKTLARPKRKSGKR"
     gene            1209..2009
                     /locus_tag="AC16CX_19685"
     CDS             1209..2009
                     /locus_tag="AC16CX_19685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3014766.1"
                     /translation="MHPMLNIAVRAARRAGNIINRATRNLDLVVFREKAANDFVSEVD
                     REAEQAIIRTLREAYPDHSILAEESGASGTSEHQWIIDPLDGTTNFIHGFPQYAVAIA
                     HKHRGVITQAVIYDPSRNDLFTATRGRGAFLNDQRIRVSKRTHLRSSLIGTGFPFRQL
                     EHVDAYLVILRDMMKGAAGVRRAGSATLDLAYVAAGRLDGFWEFGLSPWDMAAGALLI
                     AEAGGLVGDLNGENKYLETGNIVAGNPKVFVELLRAIAPHLTPALKQG"
     gene            complement(2262..2978)
                     /locus_tag="AC16CX_19690"
     CDS             complement(2262..2978)
                     /locus_tag="AC16CX_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3014767.1"
                     /translation="MLRYFDMLARGFGAPTRAAVALALILLAGCTPQAALVSALLPDG
                     TVSVLLSHLEREEDGNRKRVAELEARKDWDGLAKFAEENLAKDRNNADWWFVAGYAHS
                     QAGRHGRAIECYSERVRLAPEDMLGWNQLAQSYRDAGQPQRAVQTLNNAHLVRKGTPS
                     TYFLLGESYSDLNRYLPAAAAYREAVQQNSEFAQAWFGLGRAYSRLNRAADFEQALKA
                     LIPLNPALAKELAEMRPGPR"
     gene            3004..4785
                     /gene="mutS"
                     /locus_tag="AC16CX_19695"
                     /pseudo
     CDS             3004..4785
                     /gene="mutS"
                     /locus_tag="AC16CX_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017778052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
BASE COUNT          890 a         1624 c         1533 g          871 t
ORIGIN      
        1 aaaccagccg cgccagccac ttcaggccga cgttccacag acgatgggcc atgcggtgga
       61 acaacattgc gtggaacccg ggatagcacg tgatcacctc ccaggtattc cgggccgccg
      121 ggtcgcgttc gaaaacaacc gcgatttcct ctcgaatacg ggaaaacatc gtttacaaat
      181 caaagctaag gccgcgcagc ttacgatgcc ctagtaattt agtcaacttt agatcggtaa
      241 gcagtgaaca gtgagcggaa atattcttaa ggggggttat cgcttactgc tcgctgctga
      301 ccgctcaccg cttaccgctc ttccgcttcg ggcgggcaag cgtcttgatg atgccacgca
      361 ggatgttgac ttcctctttc tcgagctggg cgcgcccgaa cagccgccgc aggcgcggca
      421 tcaacttctt ggggtgctcg ggattgagga aaccggtttc gcgcatgctg cgctcgagat
      481 gttcgtaaaa acgctccact tcctcgtggg tggcgagcgt gcgcggcctg ccgccgttca
      541 ttgcgcctcc ctctgccgcc agccgcagct cataggcgaa cacctgcacc gccgccgcga
      601 gattgagcga ggtgtactcg ggattggcgg gtatggtcgc gagcagctgg catttattga
      661 cctccgcggc ggtcagcccg aaggtctcgt tgccaaagac cagcgccacg ggttgcgcac
      721 gcgcgacctc gataatgcgc gccgccgcct cgcgcgcgct gaccgtcggc accgcgatct
      781 cgcgggggcg cgccgtgcag gcgaccgtga acgccgagcc ggccagcgcc tcgtccaggc
      841 tcgcgcacac gcgcgcgctg cgcagcacgt cagtcgcgcc cgaagccagc cactccgcct
      901 gcggatcggg aaagcgcacg ggcgccacga gacagagccg cccgagaccc atcgtcttca
      961 gggcgcgcgc tgcggcgccg atgttgcctg gatgcgccgg cttgcacagc acgacgcgga
     1021 tgtttttgag cggatcggtg gcgttcattc aattggaacg gggtattcga ttatccgtgc
     1081 agatcacacg agtcggccat tgcagcgccc ccgataattc atgggattat cggcggcgta
     1141 cccgtaaaat accgtgttcg gtcatcccct gctgcaagct tcaattcaag accccggcgg
     1201 cgccgatcat gcatccgatg ctcaacatcg ccgtcagggc tgcgcgccgc gccggcaata
     1261 tcatcaaccg cgcgacccgt aacctggatc tcgtcgtctt cagggaaaaa gcggcgaacg
     1321 atttcgtctc ggaggtggat cgcgaggcgg aacaggcgat catccgcacg ctgcgggagg
     1381 cctatcccga tcattcgatt ctagcagagg aaagcggcgc ctccggaacc tcggaacacc
     1441 agtggatcat cgatccgctc gacggcacca ccaatttcat ccacggcttc ccgcagtacg
     1501 ccgtggccat cgcgcacaag caccgcggcg tgatcacgca ggcggtcatc tatgacccct
     1561 cgcgcaacga cttgttcacc gccacgcggg gacgcggcgc gttcctgaac gaccagcgca
     1621 tccgcgtcag caagcgaacg cacctccgct cgagcctgat cgggaccggc ttcccgttcc
     1681 gccagctcga gcacgtcgac gcctatctcg tgatcctgcg cgacatgatg aagggcgcgg
     1741 cgggcgtgcg ccgcgcgggt tccgccacgc tcgacctcgc ttacgtcgcg gcgggccggc
     1801 tcgacggttt ctgggaattc ggcctctctc cctgggacat ggccgcgggc gccctgctca
     1861 tcgccgaggc cggcgggctg gtcggcgacc tcaacggcga aaacaagtac cttgagaccg
     1921 gcaatatcgt cgccggaaat ccgaaagtgt ttgtcgaatt gttgcgggca atcgcgccgc
     1981 accttactcc ggctctgaaa caaggctgaa ccgccaagtt caaatcaggg gcagattgaa
     2041 ccgccaaggc gccaagagaa ccaaatacaa aaatagaacc gagtataaaa atctatcgag
     2101 ccacggagac agggagggca tcaaaagcag gaactgaaca aaagtacagt tctttgttat
     2161 tgattcgtgt ctttgcttct ctccgcgtct ctgcggttca agtattgatc tgcttttctt
     2221 ggcgtcttgg cggttcgtaa tcggtttctt ggcggtttgt gttagcgcgg cccgggacgc
     2281 atttcagcca gctccttcgc caacgccgga ttgagcggta tgagcgcctt gagcgcctgt
     2341 tcgaagtcgg ccgcccggtt gagccgcgag taggcccgcc ccaggccgaa ccacgcttgc
     2401 gcgaattcgc tgttctgctg gaccgcttcg cggtacgccg cggctgccgg cagataacga
     2461 ttgagatccg agtagctttc ccccagcaga aaatacgtcg aaggcgtgcc tttgcgcacc
     2521 aggtgcgcgt tgttcagcgt ctgcaccgca cgctgcggct gaccggcatc gcgatacgac
     2581 tgcgcgagct ggttccagcc cagcatgtcc tcgggcgcaa gccgcaccct ctcgctgtaa
     2641 cattcgatgg cgcgcccgtg ccgcccggct tgcgaatgcg catagccggc gacgaaccac
     2701 cagtcggcgt tgttccggtc cttcgccagg ttttcctcgg cgaatttcgc gaggccgtcc
     2761 cagtccttgc gggcttccag ctcggcgacg cgcttgcggt tgccatcctc ttcccgctcg
     2821 aggtggctca acaggacgga gacggtgcca tcgggaagca gggcgctcac cagtgccgcc
     2881 tgcggcgtgc agccggcgag caggatcagg gcgagcgcca ccgccgcgcg cgtcggggcg
     2941 ccaaaaccgc gggccaacat gtcaaaatag cgaagcatct cctgcgacga agtataaccg
     3001 ttcatgcagc agtccccgga acgccacacg cctatgatgc agcagtacct gcgcatcaag
     3061 gcgcagtacc ccggcatgct ggtgttctac cgcatgggcg acttctacga gctgttctac
     3121 gaggacgccg agcgcgcggc gcggctgctc aatatcacgc tcacgcgccg cggggaatcc
     3181 gccggcgaag ccatcaagat ggcaggggtg ccgtaccact cggtggacca gtacctcgcg
     3241 cggctggtga aactcggcga gtcggtcgcg atctgcgagc agatcggcga tcccggcgct
     3301 tcccgcggcc ccgtcgagcg tcaggtgacg cgcgtggtca cgccgggcac gctgaccgac
     3361 gccgccctgc tcgaagacaa gcgcgacaac ctgctgctcg cgctctgccc gcagcgcaat
     3421 acggtggggc tggcctggct ttcgctcgcg tccggccgtt tcgcggtgat ggagacagcc
     3481 gccgccaacg tgactgccga gctcgaacgt cttgcaccgt ccgagattct ggttccggag
     3541 gatgcggcgg ctgaattctt aaacggcccg acagctcccg taaagcgctt gccgccgtgg
     3601 cagttcgatc tggatgccgc tcggcgcgtc ctgacccgcc agttcgagac ccacgatctt
     3661 tcaggatttg gcgccgccga tctgaccgcg gcgatttccg ccgccggcgc cctgctggag
     3721 tatgcccgct ccacccaggg caccgcgatc gcccacgtca aatcgctgat ggtcgagcag
     3781 gaacgtgcct gggtccgcat ggatccggct acgcgccgca acctcgagct gaccgaaacg
     3841 atacgcggtg aacccgctcc caccttgttc tcgctgctcg acacctgcgc gaccggcatg
     3901 ggcagccggc tgctccgaca caccctgcac catccgttgc gcgatcgagc cgtgctcggc
     3961 gcacggctcg atgcagtgga aaagctcatc agcagcgatg gcgtggtgcc gtaccgcggg
     4021 ttgcatgaaa cgctcaggca atgcgtggat gtcgagcgca tcaccgcgcg tgttgcattg
     4081 aaatcggcgc ggccgcgcga cctggcggga ctgcgcgaga cgctgaagct gctgccggag
     4141 ctcggtttca tgccggccgc gctggcaagc ccgcggctcg cggagcttgc aggggcgcta
     4201 gcgccgcagg acgagctcgt tgcgctgctg aaaacagcga tcaagcccga accggccgcg
     4261 gtgctgcgcg agggcggcgt gatcgccgac ggtttcgacg ccgaactcga cgagctgcgc
     4321 gcgatccagt cgaattgcgg cgccttcctg atggagctgg aaacgcgcga gcgtgcacgc
     4381 accggcatcg ccaatctcaa ggtcgagtac aaccgcgtgc acggcttcta catcgaggtc
     4441 acccgcgcgc aatcggacaa ggtgccggac gactaccggc ggcgccagac gctcaagaac
     4501 gccgagcgct acatcacgcc ggagctgaag gcgttcgagg aaaaggcgct ctcggcgcgc
     4561 gaccgcgcgc tggcgctcga gaagtcgctc tatgacgagc tgctgagctc gctgcagcgg
     4621 ggaatacccg ccctgcaggg aatcgcccgc gccctcgcgc agctcgacgt gctgtgcact
     4681 ttttccgcca tggcttcccg cagaaattac tgcaagccgc aattcgtgaa tgagcccctg
     4741 atcgagatcg aggccgggcg ccacccggtg gtggaagcgc gcgtcggcga caggcggcag
     4801 tcgttagcga tgaagctctc gatctgcgcc tcgaccacgg catggcgtcc ggcgtcgatc
     4861 tcgatcttga cgtcatcagt gaactccggc ctggcatagc ctctcttggt cgctacgc
//
LOCUS       DBNGWZ010000630         2272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2622199, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000630 DBNGWZ010000000
VERSION     DBNGWZ010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2622199"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16CX_19700"
     CDS             complement(<1..877)
                     /locus_tag="AC16CX_19700"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR3014768.1"
                     /translation="MEILGALESLFGPAWPAVWTLAKIVAIVVPLLLGVAYLTLAERK
                     VIGYMQVRIGPNRVTFFGIPWLRGWGQPIADGLKLLVKEIIVPAGANKFLFVLAPVMA
                     IMPAMAAWAVVPFSPELVLANIDAGLLYVMAITGMGVYGIIIAGWASNSKYAFIGSLR
                     SAAQMVSYELAMGFALVCVLMAAQSLNLGEVVRMQQGGWGLANWFWIPLFPIFLVYLI
                     SGVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILISTMASVMFLGG
                     WLSPVPFLPDGFIWLA"
     gene            complement(880..2073)
                     /locus_tag="AC16CX_19705"
     CDS             complement(880..2073)
                     /locus_tag="AC16CX_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3014769.1"
                     /translation="MTWRGTQLNLINPFDDELLMRVANRAIIAPAAMPGVLAQVIKAT
                     AEQKGVQVPEAVRGAVSGTAISDAAQRIAVSLTGGKNAAILLGNLAQHHPAASHLHQL
                     GLALAEVAGARFGFLGEAANSVGGYIAGCVPFGRNAGLNAVQMVKAPLKAYLLLGVEP
                     ELDTHDPQQSAAALKAAELVVAMSPYQHRAVEYAHVLLPIAPFTETAGTFISTEGRVQ
                     SFDGVVRPLGETRPAWKVLRVLGNLLGLSGFEHDSAEDVRREALGDGDVAGKLDNRLR
                     AFVLDSGLGTRGSGLERIAEVPIYAADAIVRRARALRRTRDASPPVAWMNRALFDKLG
                     LREGDGVRVRQGNGEAVVAAGVDEKLPPDCIRLAAARPETAALGAMFGTVAAERVAAR
                     EKLAV"
BASE COUNT          401 a          812 c          719 g          340 t
ORIGIN      
        1 ccgccagcca gatgaagccg tcgggcagga acggcaccgg cgagagccag ccgccgagga
       61 acatgaccga ggccatggtc gagatcagga tcatgttcgc gtattcggcg aggaagaaca
      121 cggcgaacgc catgcccgag tactcgacgt ggaagccggc gacgatctcg gactcgccct
      181 ccgccacgtc gaacggcgcg cggttggtct cggccacgcc cgaaatgagg tacaccagga
      241 aaatcgggaa cagcgggatc cagaaccagt tggcgaggcc ccaccccccc tgctgcatcc
      301 tgacgacctc gccgagattc aggctctgcg ccgccatcag cacgcacacc agcgcgaatc
      361 ccatcgccag ctcgtacgac accatctggg cagcggagcg cagcgatcca atgaaggcgt
      421 acttggagtt cgaggcccag ccggcgatga tgatgccgta gacacccatt ccggtgatcg
      481 ccatcacata gagcagtccg gcgtcgatgt tcgcgagcac gagctcgggg ctgaacggca
      541 ccaccgccca tgccgccatc gccggcatga tcgccatcac cggcgccagc acgaacagga
      601 acttgttggc gccggccggg acgatgatct ccttcaccag gagcttcagg ccgtctgcga
      661 tcggctggcc ccagccgcgc agccacggga tgccgaagaa ggtcacgcgg ttcgggccga
      721 tgcgcacctg catgtagccg atcaccttgc gctcggccag cgtgaggtag gcgacgccga
      781 gcaggagcgg caccacgatg gccacgatct tggcgagcgt ccagaccgcc ggccaggccg
      841 ggccgaacag gctttccagc gcgccgagga tctccatgat cagaccgcca gcttctcgcg
      901 cgccgcgacg cgctcggccg ccaccgtgcc gaacatcgcg cccagcgccg cggtctcggg
      961 ccgcgccgcc gcgagccgta tgcaatcggg aggcaatttt tcatcgacgc cggcggccac
     1021 cacggcttcg ccgttgccct gccgcacccg cacgccgtcg ccttcgcgca gcccgagctt
     1081 gtcgaacagc gcacgattca tccaggcgac cggcggcgag gcgtcacgcg tgcgccgcag
     1141 ggcacgcgcg cggcgcacga tcgcgtcagc cgcataaatc ggcacctccg cgatgcgttc
     1201 gagtcccgag ccccgggtcc cgagtcccga atcgagcacg aaagcgcgca gacgattgtc
     1261 cagcttgcct gcgacgtcac cgtcgcccag cgcctcgcgt ctcacgtctt ctgcgctgtc
     1321 atgctcgaaa ccggacagtc ccagcaggtt gcccaaaacg cgcagcacct tccacgccgg
     1381 gcgggtctct cccagcggcc ggaccacccc gtcgaagctc tgcaccctgc cctcggtgct
     1441 gatgaaagtg ccggcggtct cggtgaaggg cgcgatcggc agcagcacat gtgcgtattc
     1501 gaccgcccgg tgctgatatg ggctcatcgc caccaccagc tctgcggcct tcaacgccgc
     1561 ggccgactgc tgcggatcgt gcgtatcgag ctcgggttcg acaccgagca gcaggtacgc
     1621 cttcagtggc gccttcacca tctgcacggc gttgaggccg gcgttccggc caaatggcac
     1681 gcagcctgca atgtagcctc cgacgctgtt agccgcctcc cccaggaagc caaaccgtgc
     1741 gcccgccact tcggcaagcg ccaggcccaa ttggtgcaga tgggaggcgg ctgggtgatg
     1801 ctgtgcgaga ttaccgagca ggatcgccgc gttcttgccg ccggtcaggc tcaccgcaat
     1861 gcgttgcgca gcatcggata tcgcggtgcc gctcactgcg ccgcgcacgg cctcgggaac
     1921 ctggacgccc ttctgttccg cggtggcctt gatcacctgc gccagcacgc cgggcatggc
     1981 ggcgggcgcg atgatcgccc tgttcgcaac ccgcatcagc agctcgtcgt cgaacgggtt
     2041 gatcagattg agctgcgtcc cgcgccaggt cacctgtctc agacggtggg cgatcagcgg
     2101 atggtcctat ctcagggtcg agcccaccac caggacgcga tcgagctggt tgaagtcggc
     2161 caccgccatg ccgagcctcg gctcgcccgc gagcctgccg tcggcgctga aatcggactg
     2221 gcgcagccgg aaatccacgt tgcccgaggc aaactcgcga gcgagtttgc ct
//
LOCUS       DBNGWZ010000631        27277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_231890, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000631 DBNGWZ010000000
VERSION     DBNGWZ010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 27277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27277
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_231890"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="AC16CX_19710"
     CDS             complement(<1..646)
                     /locus_tag="AC16CX_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR3014770.1"
                     /translation="MRQVRPARSHAVVRYLAILCVGLVALALFLVANSSADPALFAER
                     YPQLLALNGAVALLLMTLVVYQFVTLRRNLKAGVFGSKLTLRLVLLFALMALLPGVLI
                     YGMSVQFLARSIESWFEVRVDKALEGGINLGRGALDGMLRDLTAKADGMALALSTRPT
                     AEHLAALGALREQSGVQEATLFSESGRILAFAGNERVGLMPEPPGAAALRQMRRD"
     gene            complement(655..1185)
                     /locus_tag="AC16CX_19715"
     CDS             complement(655..1185)
                     /locus_tag="AC16CX_19715"
                     /inference="COORDINATES: protein motif:HMM:NF025692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HIR3014771.1"
                     /translation="MAALILGSAGAAPARADGIEIKKAALVAAEDGYFLEADFDIALT
                     HTLEEALNKSVPLYFTLDFELIRPRWYWLNEKVANTRQQYRLSFNALTRQYRVGVGTL
                     YQNFSSLPEALTFLSRVRLREIAEPGALGKGASYTAALRMRLDTSQLPKPFQISAVGS
                     REWSVSSDWHRWTVSP"
     gene            complement(1199..2482)
                     /gene="rsmB"
                     /locus_tag="AC16CX_19720"
     CDS             complement(1199..2482)
                     /gene="rsmB"
                     /locus_tag="AC16CX_19720"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR3014772.1"
                     /translation="MVEVQRLAATVLGGVLAGHSLDAELALAWRRHPRLAARERAAVQ
                     DLAYGTLRFRGRLEALLEALLDKPLKDARLYTLLLVALYQLEHTRAAPHAVVDHAVRA
                     CNALGLTSAKGLANAVLRNFLRVRPALNARVQRDEAARYSHPQWWIDKLRAQYPAHYT
                     AALEADNLRPPLALRVNRRRMGVPSYLDLLALHGIGGEACGPAAVLLEKALPAERIPG
                     LAEGLVSVQDVAAQLAAPLLDLRDGLRALDACAAPGGKTGHILELADVELTALDDDAR
                     RLERVHENLARLGFAAHVVCGDAREPASWWDGTPFDRILLDAPCSASGVARRHPDIKW
                     LRRESDIAQLAHAQSRMLDALWQTLASGGKLLYATCSVFREENGERVAQFLERHRDAT
                     RLTLPAVENDAQLPAGQLLPDGRHDGFFYALMQKV"
     gene            complement(2475..3401)
                     /gene="fmt"
                     /locus_tag="AC16CX_19725"
     CDS             complement(2475..3401)
                     /gene="fmt"
                     /locus_tag="AC16CX_19725"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3014773.1"
                     /translation="MRLVFAGTPEFAAVSLEALLAARHEVTLVLTQPDRPAGRGLKPR
                     PSAVKQIALARGLSLMQPSTLKEPAALNAVAAARPEALVVAAYGLMLPVELLSLPARG
                     GINVHASLLPRWRGAAPIQHALLAGDTETGVTIMRMDEGLDTGPVLQQQAVPIAPDDT
                     AGTLHDRLAALGARLLLEVLASNPAPRPQEAAGATYAPRIGKNEAEIDWRKPAAEIER
                     LVRAFDPAPGAQTRFDRTVLKVWRARVEASTAAAPGTVIAAGPEGIVVACGRGALRIT
                     ELQRAGGKRLPARAFLSGLKLGRGVHLGPGHG"
     gene            complement(3523..4053)
                     /gene="def"
                     /locus_tag="AC16CX_19730"
     CDS             complement(3523..4053)
                     /gene="def"
                     /locus_tag="AC16CX_19730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR3014774.1"
                     /translation="MSLLPILRYPDPKLHTVAAKVDRFGGRITKLINDMAETIYAAPG
                     VGLAATQVDVHLRVIVIDISETRAQLQVFVNPVLIAASGDADREEGCLSVPGVYEKVR
                     RAERVTVRAQDADGQPFTLEAEGLLAVCIQHEMDHLEGKVFVEKLSRLKQNRILARLR
                     KQDRRTAVPDGRRLAL"
     gene            4195..5328
                     /locus_tag="AC16CX_19735"
     CDS             4195..5328
                     /locus_tag="AC16CX_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3014775.1"
                     /translation="MTPFMPALAADAGALKEDAPDRYVVVPGDTLWSISGRFLKDPWK
                     WPELWKMNQEQIKNPHRIYPGDVIVLDRSGQEAQLRLVEGRGTAGRGATIKLSPKVRA
                     EPLKDAAIPAIPPSVIEPFLSRPLVVGQTELDSTPFILATQENRVAIGAGSVAYVEGL
                     TPEQGKVWQVFRRGDALVDPDTREILGYLAIYLGEARVLRFGDQSTIEITKSQQEIYV
                     GDRLLPASKDLPTFSYVPRAPLKSVRGRIVTTYGNLGETGPAGIVALSKGTADGLEVG
                     HVLAIYRSQSTAQYEMRMSALFGREGPSGSDRPRTYYSEQITPRDGPLVDSGKPIRVE
                     DISNLPDERYGLVMVFRAFDRAAFGLVMQASRPVAINDVVTNP"
     gene            5407..6483
                     /gene="dprA"
                     /locus_tag="AC16CX_19740"
     CDS             5407..6483
                     /gene="dprA"
                     /locus_tag="AC16CX_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR3014776.1"
                     /translation="MPLDEDLSAWLSLSLTPGLGGESLRRLLTAFGEPRRVLAASRAA
                     LAQHVPDGVASAIKQSRLDETVAAVIGWLEDPANHVVTLADAGYPPQLLQIPDPPPLL
                     YVKGRVELLCRPSLAIVGSRNATPQGVANAETFAQTLSDAGLTVVSGLALGIDAAAHR
                     GGLAGAASSVAVLGTGADVVYPARNRALAHELAARGALVSEFPLGVRPLAANFPRRNR
                     LISGLAYGCLVVEAAADSGSLITARLAAEQGREVFAIPGSIHSPLAKGCHALIKQGAK
                     LVESARDILEELHLPAPAVPATATDVDSSALLDALGHDPCDLDTLAARCRLPAAEIAA
                     ALTQLELDGRVASLPGGLYQRLHR"
     gene            6583..9195
                     /locus_tag="AC16CX_19745"
     CDS             6583..9195
                     /locus_tag="AC16CX_19745"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HIR3014777.1"
                     /translation="MGKLLIIAEKPSVAADIARALGGFTRHGDYYENGRYVLCSAVGH
                     LLELQASEEHDPRAGRWTFKHLPVIPPKFELAPIDERSGTRLNLLLRLMKRKDVTGLV
                     NACDAGREGELIFRYIVQYSRTAKPIERLWLQSMTPSAIREGFAALRPDRELRPLGDA
                     ARCRSEADWLVGINGTRAMTAFNNRASETGFYKTTVGRVQTPTLVILTEREERIRAFR
                     PRDYWEVHARFGTQAGEYTGRWFDPGFKKDDDSERKPERLWRAHEADAITSDCRGKTG
                     AVSEETKPATQLSPLLFDLTSLQREANGRFGYSAKTTLALAQALYERHKVLTYPRTDS
                     RALPEDYIATVKKALDVLSGIRDYAAHAKNVLKHGWVKPDRRVFDNAKVSDHFAIIPT
                     LQTPKHLNEAEQKLYDLVVRRFLAVFHPAAEYLQTTRITQVGDHHFKTEGRILQSPGW
                     LAVYGRTAQEESQNLPAVAAGEEVAVLEVSAVHNQTKPPARYTEATLLSAMEGAGKLV
                     EDDELRAAMEAKGLGTPATRAAIIEGLIHEDYVHRNGRELVPTPKAFSLLFALKHFGV
                     TEITSPELTGDWEYKLKLMEGGKLRRGEFMDHIEQVTRDLVDRIKHGDIPDEAYATVE
                     APCPKCGGVVQENYRKFQCQTCDFSLWKVSAGREWAPEEVAELIVKRFIGPLTGFRSR
                     MGKPFSAAIRLNGDYRIEFDFGQARHEEEAANPPDFSGQESLGPCPKCGARVFEHGAV
                     YVCEKAVGVSRSCDFRSGKMILQQPVERAQMQKLLASGRSDLLTRFISKKGRRFKAYL
                     VKTPEGRVGFEFEARAPRKTEAREPAAAPSYAARAERRSPTQPAATKRAKRGAKAATA
                     AASKAPPKTKRKAA"
     gene            complement(9227..11656)
                     /gene="gyrB"
                     /locus_tag="AC16CX_19750"
     CDS             complement(9227..11656)
                     /gene="gyrB"
                     /locus_tag="AC16CX_19750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR3014778.1"
                     /translation="MAIQTDEYEQQRTPDDYDASSIKVLKGLEAVRKRPGMYIGDTSD
                     GTGLHHMMFEVVDNSVDEALAGYCNEITVTIHPDNSISVVDNGRGIPTERHPEDEEGR
                     SSAEVAMTELHAGGKFDNNSYKVSGGLHGVGVSVVNALSEWLRLTIRRDGKVHQMEFR
                     MGETVAPLKATGKTEKRGTEVHFLPSKEIFGKVDFHYEILAKRLRELAFLNSGLKILL
                     IDQRTAKEETFAFTGGVKGFVEYINRSKNVLHPNIFYSKVEKDGIVVEVAMQWNDSYQ
                     DEVQCFTNNIPQRDGGTHLTGLRAAMTRTLNQYIESSKLADKAKVVTSGEDMKEGLAA
                     VLSVKVPEPKFSSQTKDKLVSSEVRPVVDEIVAQKLGEFLLEKPGDARIICSKIVDAA
                     RAREAARKARELTRRKGVLDSFGLSGKLADCQERDPAQSELYIVEGDSAGGSAKQGRD
                     RKFQAVLPLKGKILNVEKARVDKVLSSQEIITLISVLGTGIGEEYAPERLRYHRIIIM
                     TDADVDGSHIRTLLLTFFYRQMRQLIEDGRIYIAQPPLYKVKHGKSERYLKDEHELKE
                     MLLNLALSEAELHTAVNGQTLSKDSLEKVAKEYLLAEAVIERLSRLIDEDVLHALMRR
                     PVQFDLSDEKGAQKSARELAPLLAPMGMKVEAKYDDKTERNVLLVSRTQHGIVHHSHI
                     DADFVQSGDYGQIRKTAQVLQGLLGEGAYVKRGDQQHPVKEFRDAIDWLLDEVQRGIG
                     IQRYKGLGEMNPEQLWETTMDPNTRRLLKTRIEDAINAGEIFSTLMGDEVEPRRNFIE
                     ANALGVRNLDI"
     gene            complement(11667..12776)
                     /gene="dnaN"
                     /locus_tag="AC16CX_19755"
     CDS             complement(11667..12776)
                     /gene="dnaN"
                     /locus_tag="AC16CX_19755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3014779.1"
                     /translation="MNLVQLERDALLKPLQAVTGIVEKRHTLPILSNVLIERKQGRLL
                     LVATDLEIQVATSTDTGKGTGEDVSLTVSARKLQDILRALPEATEATLDVQNNRLQVR
                     SGKSRFNLQTLPAADFPAMADPGAAQAKVTMPQSALRELLLLVQYAMAQQDIRYYLNG
                     LLLVLDEGQLKVVATDGHRLSYAARPLGQQQEKREVILPRKAVLELGRLLGDAEQAVV
                     VELFPSMVRFSFADVVLTTKIIDGKFPDYTRVVPTNYQKHFTIKRQELLQSLQRAGIL
                     SNEKFRGVRWMIGSNSLRISCTNNEQEEAQEELEIAYGGEALDIGFNITYLLDVLNNV
                     QSESVECAFGDANSSMLVTVPDNREFRYVVMPMRI"
     gene            complement(12976..14313)
                     /gene="dnaA"
                     /locus_tag="AC16CX_19760"
     CDS             complement(12976..14313)
                     /gene="dnaA"
                     /locus_tag="AC16CX_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3014780.1"
                     /translation="MNSFWRFCLTYFEKELPPQQYQTWIKPLRFHADGDRLTLVAPNR
                     FVLQWIRDKFLPRIELLAREQLKRNAEVLLTLGDKEIAPAPPLHLPRQQAQKDRGAKP
                     SSREVSRLNPAFAFETFVTGKANELARAAAAQVAERPGAAYNPLFIYGGVGLGKTHLI
                     QAIGNRLREQAPETKIRYIHAEQYVSDVVRAYQHKAFDDFKRYYHSLDLLLIDDIQFF
                     SGKSRTQEEFFYAFNALVEAHKQVVITCDTYPKEIAGMENRLISRFGWGLTVAVEPPE
                     LEMRVAILLKKAEAEGVRLDEAVAFFLAKHIQSNVRELEGGLKRVLAYARFSGRELSV
                     ELCREALRDLLAIQHRQVSIENIQKTVADYYKIKVAEMYSKKRSRNVARPRQIAMALA
                     KELTQLSLPDIGEAFGGRDHTTVLHACRKVALLKSTNSELTRDFNSLLNVLRS"
     gene            14506..14640
                     /gene="rpmH"
                     /locus_tag="AC16CX_19765"
     CDS             14506..14640
                     /gene="rpmH"
                     /locus_tag="AC16CX_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3014781.1"
                     /translation="MKRTYQPSVTRRKRTHGFRARMRTRGGRAVLRARRAKGRARLSV
                     "
     gene            14661..15005
                     /gene="rnpA"
                     /locus_tag="AC16CX_19770"
     CDS             14661..15005
                     /gene="rnpA"
                     /locus_tag="AC16CX_19770"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3014782.1"
                     /translation="MPRSARLLKREQYRAALAAGAAKIRRHFTLYVRPNGLSLARIGI
                     IASKRVASRAIDRNRAKRLVREAFRKARHRLGGVDVVVELRRCPAREFSMAAGAEIAR
                     LLEEIGTLSHCG"
     gene            15024..16679
                     /gene="yidC"
                     /locus_tag="AC16CX_19775"
     CDS             15024..16679
                     /gene="yidC"
                     /locus_tag="AC16CX_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015856163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR3014783.1"
                     /translation="MDSQRLILFFVFSFSVFLLLDAWQRDQPPPQPTAPVSDKTGKQP
                     PAAVQPPVPTGKPAIPQPAVPEQERRAPEASTAIQVETDLYVAQINTRGGDLSRLQFS
                     KHRDTLDRRKDFVLFESTVERTYVAQSGLIGSGLPNHRTAYAAAGNSYQLAEGQDSVE
                     VRLTAPAADGVQVTKVYRFRRGSYLIDVGFEINNGSAVAMQPHAYFQMVRDGKPPAGD
                     SAMLPTFTGVAVYTDKEKFKKLPFADIDKGKVPYPKHGNDGWIAMLQHYFFSAWLPKN
                     GTAREFYTRRLDENLYSAGVIVPVGLVNAGASAAIAVPLYAGPEEQEKLAKIAPGLDL
                     TIDYGWLTVIAVPLFWVLAWLKQWVGNWGVAIILLTVIIKLLFYPLSAASYRSMAKMR
                     VLAPKLQRLKDQYGDDRQRLQQAMMELYKTEKINPLGGCMPIVVQIPVFIALYWVLLA
                     SVELRHAPFTLWIDDLSAPDPWFILPILMALTMFVQTWLNPEPPDPVQARVMKIMPIV
                     FSIFFFFFPAGLVLYWLVNNVLSIAQQWRITRVLEREAAPRKN"
     gene            16725..18065
                     /gene="mnmE"
                     /locus_tag="AC16CX_19780"
     CDS             16725..18065
                     /gene="mnmE"
                     /locus_tag="AC16CX_19780"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR3014784.1"
                     /translation="MTENDTIAAIATAHGFSGIGVVRVSGPRLDSVIADLVGKPLPPR
                     HAVLAEFHDTRGEVIDQGIALYFPGPRSYTGEDVLELQGHGGPVVLQQLLKRCLELGA
                     RPARPGEFTLRAYLNDKLDLAQAESVADLINAATAEAARGALRSLQGVFSQKIEELVR
                     RLVELRALVEATLDFPEEEIEFLKQIDGEGRLAIIRAKLQDLLAASQQGSLLREGIHI
                     VLAGQPNVGKSSLLNRLAGEELAIVTEVPGTTRDAIRQSINLEGIAAHIIDTAGLRDS
                     SDPVERIGIERTWAAIEKADLVLLLIDATQGESNADRNILNRLPSKLPCIRVMNKIDL
                     LTGPASLERRDGRDRVWLSAKTGAGIDLLRNTLLEMVGWQGRGEGLFMARERHVHALK
                     TAQFHLESAAQQGRQLELFAEELRLAQECLGTITGEFTADDLLGEIFSQFCIGK"
     gene            18226..20163
                     /gene="mnmG"
                     /locus_tag="AC16CX_19785"
     CDS             18226..20163
                     /gene="mnmG"
                     /locus_tag="AC16CX_19785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR3014785.1"
                     /translation="MQAIEKYDVIVVGGGHAGTEAALATARMGCETLLLTHSIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALDGAMAAATDEAGIQFRILNSRKGPAVRATRAQADRL
                     LYKQAIRGRLENQHRLRIFQQAVDDLLLEGDRVTGVVTQIGLKFSARAVVLTTGTFLA
                     GLIHVGLKNYQAGRAGDPPALTLAQRLRELKLPVGRLKTGTPPRIDGRSIDFSVMAEQ
                     PGDEPTPVFSFLGRREQHPPQLPCWITHTNARTHEIIRNALDRSPLYTGVIEGVGPRY
                     CPSIEDKVMRFASKASHQIFLEPEGFTVNEFYPNGISTSLPYDAQQELVRSIRGLERA
                     HITRPGYAIEYDYFDPRGLKRSLETKAISGLFFAGQINGTTGYEEAAAQGILAGINAA
                     LFVKEHEPWCPRRNEAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADLRLTE
                     TGRRLGVVGDARWGAFSRKREAIAREQERLKSTWINPRLLPPETLASVFIQPIGREQP
                     LTELLRRPDVSYAQLMELPGAGPGVSDPQVAEQVEIQAKYQGYIERQSEEIARHQHHE
                     NLRLPAGVDYREVRGLSVEAQQKLNEHRPETLGQASRISGITPAAISVLLVYLKRRLA
                     GTHFGKPANPGARGRTATKTA"
     gene            20144..20788
                     /gene="rsmG"
                     /locus_tag="AC16CX_19790"
     CDS             20144..20788
                     /gene="rsmG"
                     /locus_tag="AC16CX_19790"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3014786.1"
                     /translation="MQLRQRDISLAAQLAEGVAQLDLALPPQAQSRLLDYLGLLRKWN
                     RIYNLTAVREASRMVSHHLLDCLAVVPHVAASTILDVGSGAGLPGIPLALALPRAHVT
                     LLESSHKKTAFLQQAKFELKLDNVAVVCERLELWRPGRAFELVISRAFSDLAEFAALA
                     GRHVVPGGRLAAMKGVYPHEELAGPLHGWRLQKAIPLTVPGLRAQRHLLLLERE"
     gene            20791..21558
                     /locus_tag="AC16CX_19795"
     CDS             20791..21558
                     /locus_tag="AC16CX_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3014787.1"
                     /translation="MPRILAITNQKGGVGKTTTGVNLAASLAAARRRVLLVDIDPQGN
                     ATMGSGVDKRALGATVYHVLLGTRRIAEVRARSETGEYDLVPANRELAGAEVELVEVE
                     HRETRLKDAIASVADEYDFILIDCPPALNLLTVNGLTAAQAVIIPMQCEYYALEGLSD
                     LVQTIKKVRAHLNPGLEIEGLLRTMYDSRNTLAQQVSDQLLEHFGDKVYRTVIPRNVR
                     LAEAPSFGVPALKFDKNSKGAQAYLALAGEMLNRAEG"
     gene            21570..22415
                     /locus_tag="AC16CX_19800"
     CDS             21570..22415
                     /locus_tag="AC16CX_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3014788.1"
                     /translation="MAKIKGLGRGLDALLGSVEEPSASETLTTLAVEALQPGRYQPRT
                     RMDPEALAELAESIKAQGVMQPILARPMGAGRYEIVAGERRWRAARMAGLATVPALVR
                     EVPDRHALAIALIENLQREDLNPLEEAAGVKRLIEEFGMTHAEAAEGLGRSRAAITNA
                     LRLLELAPPVQELMREGKLDMGHARALLALPALKQIELAREAAAKQLSVRQVEQKVAR
                     LARRATVGGRPRTDRDVARLEEEWSQRLGTTVRIEAGAKPGKGRLVIHYSTLDQLDAL
                     LRKLG"
     gene            22521..22889
                     /locus_tag="AC16CX_19805"
     CDS             22521..22889
                     /locus_tag="AC16CX_19805"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR3014789.1"
                     /translation="MLRALGKPIRTVLRWQLLATAALMLVAGAWAGAHGALSAALGGS
                     VSIFSGFTSAIVAALGKAESAGGVVVTALTAEAVKIGLIVMLLWLVLANYDGVVVHAF
                     IGSFLVTVLIFAMAFFVREY"
     gene            22908..23717
                     /gene="atpB"
                     /locus_tag="AC16CX_19810"
     CDS             22908..23717
                     /gene="atpB"
                     /locus_tag="AC16CX_19810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3014790.1"
                     /translation="MAAGKELNPSDYIQHHLTFFRAPLGEGEFWALNIDTVVTSVLLG
                     LVGLGFIWWVVRGATPGVPDKRQAFVELLIEFVDDQVKGIFHHGDRHRFIAPAALTVF
                     VWVVLMNAMDFLPVDWVAGALGIVGLHEWRPVPTADVNATFALSVSVLLLSIYFAVAV
                     KGLGGWIHELFCAPFGANPLLWPANLLFNLVEYVSKTLSHAMRLFGNIYAGEILFFLL
                     WLWAATGLVGSFFGFLFGLGWAIFHILIVLLQAYIFMMLTIVYISMAHEHH"
     gene            23761..24033
                     /gene="atpE"
                     /locus_tag="AC16CX_19815"
     CDS             23761..24033
                     /gene="atpE"
                     /locus_tag="AC16CX_19815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HIR3014791.1"
                     /translation="MEKLQLLAMVQAYTSIGIGLMVGLGAAGACIGIGIMSSKFLEAA
                     ARQPELTNSLQGRVFLLLGLIDASFIIGLGIAMFFAFASPLATPFR"
     gene            24068..24541
                     /locus_tag="AC16CX_19820"
     CDS             24068..24541
                     /locus_tag="AC16CX_19820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3014792.1"
                     /translation="MSFNLTLITQMLAFAAFILFTVKVVWPYLLRAIEERQKTIADGL
                     AAAEQGKRTLEISTRQAEEAIKQARARTAEIIAQAEKRAAQMIDEARMHAKEEGNREK
                     VAAQAEIEQQVTRAKEALREQVAALAVAGAERILRREVDAKTHANLLARLKQEMR"
     gene            24546..25079
                     /locus_tag="AC16CX_19825"
     CDS             24546..25079
                     /locus_tag="AC16CX_19825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR3014793.1"
                     /translation="MAETVTIARPYAEAVSALADAGGALGKWSQMLAVMATVAATPEM
                     KAATGDPNLTSDQIYGLFAGACGDLTTEAQNFVRVLIENDRLAALPEIRDIFKELKNE
                     REGVVDAVITTAYPLDGGQLAALVAELEGRFKRKVNPQVNVDPELIGGAHVQVGDEVI
                     DGSVRGKLAEMAAALMK"
     gene            25097..26647
                     /gene="atpA"
                     /locus_tag="AC16CX_19830"
     CDS             25097..26647
                     /gene="atpA"
                     /locus_tag="AC16CX_19830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3014794.1"
                     /translation="MQQLKPSEISDLIRSRIQSLALAADIRTQGTVVSVTDGICRVHG
                     LSEVMQGEMLEFPKNTFGLALNLERDSVGAVIMGEYEHITEGDTVKCTGKILAVPVGP
                     ELLGRVVNSLGQPIDGKGPVPAKMTDVIEKVAPGVIWRKSVSQPVQTGLKAIDSMVPI
                     GRGQRELIIGDRQTGKTAVAVDTIINQKGKELICVYVAIGQKASTIANVVRKLEEHGA
                     MAYTIVVAASASESAAMQYIAPYSGCTMGEYFRDRGQDALIIYDDLTKQAWAYRQISL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNEQYVENFTKGAVKGRTGSLTALPVIETQ
                     AGDVTAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKIMKRRDT
                     GGGVRLALAQYRELAAFAQFASDLDEATRKQLERGRMVTELMKQPQYQPLQVWEMALT
                     LFAVNNGYFDDIDVKKALSAERSMRDYVKDNYSDLMKRMEDVKDLSKDDEQKLHEAIK
                     DWKKNGSF"
     gene            26754..>27277
                     /locus_tag="AC16CX_19835"
     CDS             26754..>27277
                     /locus_tag="AC16CX_19835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR3014795.1"
                     /translation="MPGTKEIRMKIRSVQNTRKITKAMEMVAASKMRRAQERMRAARP
                     YGEKIRNVAAHISHANPEYRHPFLISRDTVKRVGIILITTDKGLCGGLNTNVQRLALS
                     RIKEWEAQGESLEVCCIGNKGLGFMQRLGANIVSQVTQLGDRPQMEKLIGTVKVMLDG
                     YIQDRFDRLMIFYTR"
BASE COUNT         5029 a         8688 c         8703 g         4857 t
ORIGIN      
        1 ggtcgcgccg catctgccgc agtgccgcgg cgcccggcgg ctccggcatc agccccacgc
       61 gctcgttgcc ggcgaaggcc aggatcctgc cgctctcgct gaacagcgtc gcctcctgca
      121 cgccggattg ctcgcgcagc gcacccagcg cggccaggtg ctccgccgtc gggcgtgtcg
      181 agagcgcaag cgccatgccg tcggccttcg ccgtgaggtc cctcagcatg ccgtcgagcg
      241 cgccgcgccc gagattgatt ccgccttcga gcgccttgtc cacgcgcacc tcaaaccagg
      301 actcgatcga gcgcgccagg aactggaccg acatgccgta gatcagcacg cccggcagca
      361 gcgccatcag cgcgaacagc agcaccaggc gcagcgtcag cttggagccg aacacgccgg
      421 ccttgagatt gcgcctgagc gtgacgaact gatagacgac gagcgtcatg agcaggagcg
      481 ccaccgctcc attcagcgcc agcagctggg gatagcgctc ggcgaacagc gcggggtcag
      541 cgctcgagtt ggcgacgaga aacagagcga gcgcgaccag ccccacgcac aggatggcca
      601 ggtagcgcac cacggcatgc gaccgcgccg gcctgacttg ccgcattgcc gggctcatgg
      661 cgacaccgtc cagcggtgcc agtcggagct cacgctccac tcgcgcgagc cgaccgcgct
      721 gatctggaac ggtttcggca actgggaagt gtcgagccgc atgcgcaggg cggcggtgta
      781 actcgcgccc ttgccgagcg cgcccggctc tgcgatctcg cgcagccgca cccggctcag
      841 aaaggtgagc gcctcgggca gcgaactgaa attctggtag agcgtgccga ccccgacgcg
      901 gtactggcgc gtcagcgcgt tgaacgacag gcgatactgc tgccgtgtgt tggcgacttt
      961 ttcgttgagc cagtaccagc gcggccggat cagttcgaag tcgagcgtga aatagagcgg
     1021 cacgctcttg ttgagcgcct cctccagggt gtgggtgagc gcgatgtcga aatcggcctc
     1081 gaggaaatag ccgtcctcgg cagcgaccag tgccgccttc ttgatctcga tgccatcggc
     1141 ccgggccggg gccgcgccgg cgctgccgag gatgagcgcc gccaacgcgg caaaccagct
     1201 aaaccttctg cataagcgcg tagaagaagc cgtcatgccg tccgtccgga agcagttggc
     1261 ccgctggcaa ttgtgcgtcg ttttcaaccg cgggaagtgt caggcgcgtc gcgtctcgat
     1321 ggcgctcaag aaactgcgca acccgctcgc cgttctcttc gcggaatacg gaacaggtgg
     1381 cgtacagcaa tttaccacca ctggcgaggg tctgccacag cgcatccagc atgcggctct
     1441 gcgcgtgcgc gagctgcgca atgtcggatt cgcgccgcaa ccacttgatg tcgggatgcc
     1501 gccgcgcgac gcccgaagcg ctgcacggcg catcgagcag gatgcgatcg aacggtgttc
     1561 catcccacca ggatgccggc tcgcgcgcat cgccgcagac gacgtgcgct gcaaatccca
     1621 gccgcgccag gttctcatgc acccgttcca gtcgcctggc atcgtcgtcg agcgcggtga
     1681 gttccacgtc ggccagctcc aggatgtggc cggtcttgcc gccaggcgcc gcgcatgcgt
     1741 cgagcgcacg caggccgtcg cgcaggtcga gcagcggcgc ggcgagctgg gccgcgacat
     1801 cctgcaccga caccagtccc tcggcgagcc cgggtatgcg ctcggcgggc agcgctttct
     1861 ccagtagcac ggcggccggc ccgcacgcct cgcccccgat gccgtgcaga gcgaggagat
     1921 ccagatagct cgggacgccc atgcggcggc ggttcacgcg cagcgccaga ggcgggcgca
     1981 ggttgtccgc ctcgagcgcc gcggtgtaat gcgcgggata ctgggcacgc agcttgtcga
     2041 tccaccattg cggatgggaa taacgcgcgg cctcgtcgcg ctgcacgcgt gcattcaagg
     2101 ccgggcgcac gcgcagaaaa ttgcgcagca ccgcgttggc aagccccttg gctgaagtga
     2161 gcccgagggc gttgcacgcg cgcactgcgt gatccacgac cgcgtgcggc gcggcgcgag
     2221 tgtgctcgag ttggtagagc gcgaccagca acagggtgta cagccgcgca tccttgagcg
     2281 gtttgtcgag cagcgcttca agcagcgctt caagccggcc gcggaagcgt agcgttccgt
     2341 aggcgagatc ctggaccgcg gcgcgctcgc gggcggcgag ccgggggtgc cgccgccagg
     2401 cgagagcaag ctcggcatcg aggctgtgcc cggcgagcac gcccccgagc acggtggctg
     2461 ccaatcgttg gacctcaacc atggccgggc ccgagatgca ccccgcgccc cagcttgaga
     2521 cccgacagga aagcccgcgc cggcagtcgc ttgccgccgg cgcgctgcag ctcggtgatg
     2581 cgcagcgcgc ccctgccgca agccaccacg ataccctccg gccccgccgc aattacggtg
     2641 cctggtgcgg cggccgtgct cgcctcgacg cgggcacgcc atactttgag caccgtgcga
     2701 tcgaatcggg tctgcgcgcc gggcgcggga tcgaatgcgc gcaccaggcg ctcgatctcc
     2761 gcggccggct tgcgccagtc gatctccgcc tcgttcttgc cgatgcgcgg cgcgtacgtg
     2821 gcgccagcgg cttcctgcgg tcgcggcgcg ggattggatg ccagcacttc gagcagcagc
     2881 ctcgcgccaa gcgcggccag ccgatcgtgc agcgtgccgg cggtatcgtc aggcgcgatc
     2941 gggacggcct gctgctgcag cacgggcccg gtatcgagac cttcatccat tcgcatgatg
     3001 gtcacgcccg tctcggtgtc gccggcgagc agcgcgtgct ggattggcgc agccccgcgc
     3061 cagcgcggga gcagtgaagc atgcacgttg ataccgccgc gggcgggcag gctgagcagc
     3121 tccaccggca gcatcagccc gtaggcggcc accaccaacg cttcggggcg cgccgctgcc
     3181 accgcattca gcgccgccgg ctccttcagc gtggagggct gcatcaggga aaggccgcgg
     3241 gcgagcgcaa tctgcttcac cgccgaaggc cgcggcttca ggccgcggcc ggccggccgg
     3301 tccggctggg tgaggacgag cgtgacttcg tgcctcgcgg cgagcagggc ctcgagcgat
     3361 actgccgcga attcgggcgt tcctgcgaat acgagcctca tcgcggccgc cccggcagga
     3421 gacccaagct gagcatgggc gcgctacgga aagagtgggc tgcgccgagc cgcgcacgcc
     3481 gatcggcgcg ccggctgcga taccggaaga cgcaaatggg gatcagagcg cgagtcgccg
     3541 gccgtccggc accgccgtcc gcctgtcctg cttcctgagc cgggcgagga tgcggttttg
     3601 cttgagacgc gagagcttct cgacgaatac cttgccctcg aggtgatcca tttcatgctg
     3661 gatgcatacc gcgagcagcc cctccgcctc cagcgtgaat ggctggccgt cggcgtcctg
     3721 cgcgcgcacc gtcacgcgct cggcgcgccg cactttttca tacacccccg gcaccgaaag
     3781 gcaaccctct tcgcgatcgg cgtcgccgct ggcggcgatc aataccgggt tgacgaacac
     3841 ctgcaactgg gcgcgagttt ccgaaatatc gatcacgatg actcgcagat gcacgtccac
     3901 ttgtgttgcg gccaggccaa cccccggcgc ggcatatatg gtttctgcca tgtcgttgat
     3961 tagtttagta attcttcccc cgaaccggtc caccttggcg gcgacggtgt gcaactttgg
     4021 gtccggatag cgcaggatgg ggagcaagga cataaaaaag cttgctaatt taatcagcta
     4081 gatgcagaat ttaaaccaat atctcgggcg cagttccgtc gttggggctg cgcctgtcag
     4141 tctcttaatt gggggcgttc catgcgcaag acaattatat ccctgatttt gctgatgact
     4201 ccgttcatgc ccgcgctcgc tgccgatgcc ggcgcgctca aggaagacgc acccgatcgt
     4261 tatgtcgtgg tcccgggaga cactttgtgg tccatatccg gccgattcct caaggatccc
     4321 tggaaatggc cggaactgtg gaagatgaac caggagcaga tcaagaatcc gcatcgcatc
     4381 taccccggcg acgtcatcgt gctggaccgc tcaggtcagg aagcccagtt gcgtctggtt
     4441 gagggccgcg gcactgcagg ccgaggcgcg accatcaaac tttcgcccaa ggtacgggcc
     4501 gagccgctca aagacgcggc gataccggcc atcccgccat cggttatcga gccgttcctg
     4561 tcccgcccgc tggtggtcgg gcaaaccgag ctcgacagca cgccgttcat tctcgctacg
     4621 caggaaaacc gcgtcgcaat cggcgccggc agcgtggcct atgtcgaggg cctgactccg
     4681 gaacagggca aggtctggca ggtgttccgc cgcggcgatg cgctggtgga cccggacacg
     4741 cgcgagatac tcggctatct ggcaatctac ctcggggaag cgcgggtgtt gaggttcggc
     4801 gaccaaagca ccatcgagat caccaaatcg cagcaggaga tatacgtcgg cgatcgactg
     4861 ctgccggcat cgaaagacct ccccactttt tcgtacgtgc cgcgcgcgcc gctgaaatcg
     4921 gtgcgcggcc gcatcgttac gacctacggc aacctgggag aaaccggccc ggccggcatc
     4981 gtggcgcttt ccaaggggac ggcggacggg ctcgaggttg ggcacgtgct ggcgatctac
     5041 cgcagccaga gcaccgcgca atacgagatg cgcatgtcgg cgctcttcgg ccgggaaggg
     5101 ccgagcggca gcgatcggcc acggacctac tacagcgaac agataacccc ccgcgacgga
     5161 ccgctcgtcg acagtggaaa gccgatccgt gtggaggata tctccaatct gccggatgag
     5221 cgctacgggc tggtgatggt attccgcgcc ttcgaccgcg ccgcgtttgg cctcgtgatg
     5281 caggcctcgc gcccggtggc gatcaacgac gtcgttacca atccctgaac cggcgggagg
     5341 atcgcctccc gcgcgccgcc cccatgccgt tccgggccgg cgcacgtcat tctgtcttcc
     5401 ggcgccatgc cgctcgacga ggatctctcc gcgtggctca gtctgagcct caccccggga
     5461 ctgggcggcg agagcttgcg ccggctgctt accgcattcg gtgagccccg gcgcgtgctt
     5521 gcggcaagcc gggccgcgct cgcgcagcat gttccggacg gggtcgcctc cgcgatcaag
     5581 cagtcccgcc tggatgaaac cgtcgccgcc gtcatcggct ggctggagga tcccgccaat
     5641 cacgtcgtga cgctcgcgga cgctggctat cccccgcagc tcctgcagat tcccgatccc
     5701 cctccgctgc tttatgtcaa aggacgcgtc gagttgctct gccggccgtc gctcgccatc
     5761 gtcggcagcc gcaatgcgac cccgcagggc gtggccaacg ccgagacctt cgcgcaaacg
     5821 ctctccgacg ccggtttgac agtagtgagc gggcttgcgc tcggcatcga cgccgcggcg
     5881 caccgcggcg gcctggccgg cgccgcgagc agcgtggcgg tgctcggcac cggcgccgac
     5941 gtcgtctacc cggcccgcaa ccgcgcgctc gctcacgagc tcgcagcgcg gggcgcgctc
     6001 gtgtccgagt tcccgctcgg cgtgcgaccg ctcgcggcca attttccgcg ccgcaaccgt
     6061 ctgatcagcg ggctcgcata cggctgcctc gtggtggaag cggcggcgga cagcggctcg
     6121 ctcatcacgg cgcgtctcgc cgccgagcag ggcagggaag tgttcgcgat tcccggatcg
     6181 atccactcgc cgctcgccaa aggctgccat gccctgatca agcagggcgc aaagctggtg
     6241 gaaagcgcgc gcgatatcct ggaagagctg catctgccgg cgccggcggt tcccgcgacc
     6301 gccaccgacg ttgactcgag cgcgctgctc gacgcgctgg gccacgatcc ctgtgatctc
     6361 gatacgctcg ccgcgcgctg ccgcctgccc gccgccgaaa tcgccgcggc tctcacgcaa
     6421 ctcgagctcg atggtcgggt cgcttccctg cccggcggcc tgtatcaacg cttgcaccgt
     6481 tagttttccg cggtggctcc cgccgcgtaa aacttgcctc gaccgtcttt tgtacttatc
     6541 atcttgcgct ctttttgcca cgctggcgcc ttcgacgcgc gcatgggaaa gctgctgatc
     6601 atcgccgaaa aaccttcggt cgccgccgac atcgcgcgcg cgctcggcgg cttcactcgc
     6661 cacggcgatt actacgaaaa cgggcgctac gtgctctgct ccgcggtcgg ccacctgctc
     6721 gaactgcagg catccgaaga acacgacccg cgtgcgggcc gctggacctt caagcacctg
     6781 ccggtgatcc cgcccaagtt cgaacttgcc ccgatcgacg agcgcagcgg cacccgtctc
     6841 aaccttctgc tcaggctcat gaagcgcaag gacgtgaccg gcctcgtcaa tgcgtgcgac
     6901 gcgggccgcg agggcgagct catcttccgc tacatcgtcc agtactccag gaccgccaag
     6961 ccgatcgagc gtttgtggct gcagtcgatg acgccgtcgg cgatacgcga gggcttcgcg
     7021 gccctgcgtc cggaccggga gctgcgcccg ctgggggatg ccgcgcgctg ccgctccgag
     7081 gccgattggc tggtcggcat caacggcacg cgggcgatga cggccttcaa caaccgcgcg
     7141 agcgaaaccg gtttctacaa gaccaccgtc ggacgcgtgc agacgccgac gctggtgatc
     7201 ctgaccgagc gcgaggaacg catccgggct ttcaggccgc gcgactattg ggaagtgcac
     7261 gcgcgcttcg gcacccaggc cggcgaatac accggccgct ggttcgaccc cggcttcaag
     7321 aaggatgacg actccgagcg caagcccgag cggctgtggc gcgcgcacga agccgatgca
     7381 atcacctcgg actgccgcgg caaaaccggc gccgtcagcg aagagaccaa gcccgcgacc
     7441 cagttgtcgc ctttgctgtt cgacctcacc agcctgcaac gcgaggccaa cggccgtttc
     7501 ggctactcgg cgaagaccac gcttgcgctg gcgcaggcgc tctacgagag acacaaagtc
     7561 ctcacctacc cgcgcaccga ctcgcgcgcg cttcccgagg actacatcgc cacggtgaag
     7621 aaggcactgg acgtgctctc cggcatccgt gactacgctg cgcacgccaa aaatgtgctc
     7681 aagcacggct gggtgaaacc cgatcgccgc gtcttcgaca atgccaaggt gagcgatcat
     7741 tttgccatca tccccacgct gcagacgccc aagcacctga acgaagccga gcagaaactc
     7801 tacgacctcg tggtgcggcg cttcctcgcc gtgtttcacc cggccgccga gtacctgcag
     7861 acgacgcgca tcacccaggt cggcgatcat cacttcaaga ccgaaggccg catcctgcaa
     7921 agccccggct ggctcgcggt ctacggccgc accgcgcagg aagaatccca gaacctgccg
     7981 gccgtcgcgg ccggggagga agtcgcggtg ctcgaggtga gcgccgtcca caaccagacc
     8041 aaaccgccgg cgcgttacac tgaagcaacg ctcctctccg ccatggaggg cgctggcaag
     8101 ctggtcgagg acgacgagtt gcgcgccgcc atggaagcga aagggctcgg cacgccggcc
     8161 acgcgcgcgg cgatcatcga gggactgatc cacgaagatt acgtgcaccg caacggcagg
     8221 gagctggtgc ccacgcccaa ggcgttctcc ctgctgttcg cgctcaagca tttcggcgtc
     8281 accgaaatca cctcgccgga actgactggc gactgggaat acaaactgaa gctcatggag
     8341 ggcggtaagc tgcggcgcgg ggaattcatg gatcacatcg agcaggtcac gcgcgacctg
     8401 gtggaccgca tcaagcacgg cgacatcccc gacgaggcct acgcgacggt ggaggccccc
     8461 tgcccgaaat gcggcggcgt ggtgcaggag aattaccgca agttccagtg ccagacatgc
     8521 gatttttccc tgtggaaggt cagcgccgga cgcgaatggg cgccggagga ggtggccgaa
     8581 ctgatcgtga agcgtttcat cggcccgctc accggctttc gcagccgcat gggaaagccg
     8641 ttctcggcgg ccatccggct gaacggcgac tatcgcatcg agttcgactt cgggcaggcc
     8701 cggcacgagg aggaagcggc caatcctccc gatttcagcg gccaggagag cctggggccc
     8761 tgtcccaaat gcggcgcgcg cgtgttcgag cacggtgcgg tctacgtttg cgagaaggcg
     8821 gtcggcgtct cccgcagctg cgatttccgt tcgggcaaga tgatcctgca gcagcccgtg
     8881 gaacgcgcgc aaatgcagaa gctgctcgcc agcggccgca gcgacctgct gacgcgtttc
     8941 atctcgaaga aggggcggcg gttcaaggcc tacctcgtga agacgcccga ggggagggtg
     9001 ggattcgagt tcgaggcgcg cgcaccgcgc aagacagaag ctcgcgaacc tgctgcggca
     9061 ccgtcttacg cagcgcgagc ggaacgccgt tcgcccaccc agcctgccgc cacgaaacgg
     9121 gcaaagcgcg gcgccaaggc cgccacggcg gcggcatcca aggcgccgcc caagaccaag
     9181 cgcaaagccg cctgagtaaa taaacgcggc cgcgcgccgc gtttattcag atgtcgaggt
     9241 tgcgaacgcc gagcgcgttc gcctcgatga aattgcgccg tggttcaacc tcgtcgccca
     9301 tcaacgttga aaagatctcc ccggcgttga ttgcatcctc aatgcgtgtc ttgagcaatc
     9361 gtcgcgtatt agggtccatg gtggtttccc acagctgctc cggattcatc tcgcccagcc
     9421 ccttgtagcg ctggatcccg atcccgcgct gcacctcgtc cagcaaccag tcgatcgcat
     9481 cgcgaaactc tttcaccggg tgctgttggt cgccgcgttt gacgtaggcg ccctcgccga
     9541 gcagcccttg cagaacctga gcagtctttc tgatctgacc gtagtcgccg ctctgcacga
     9601 aatcggcatc gatatggctg tgatgcacga tgccatgctg cgtgcggctc accaggagca
     9661 cattgcgctc ggtcttgtcg tcgtacttcg cttccacttt catgcccatg ggcgcgagca
     9721 ggggggcgag ctccctggcg cttttctgcg cgcccttctc gtcggacaga tcgaactgga
     9781 cgggccggcg catcaacgcg tgcagaacgt cctcgtcaat cagccggctc agccgctcga
     9841 tcaccgcctc cgcgagcagg tattccttgg ccactttctc caacgagtcc ttgctcagcg
     9901 tctggccgtt gacggcggtg tgaagctcgg cttcggacag cgccagattg agcagcattt
     9961 ccttcagctc gtgctcgtcc ttcagataac gctcgctttt gccatgcttc accttgtaga
    10021 gcggcggctg ggcgatgtag atgcgcccat cctcgataag ttgccgcatc tgacggtaga
    10081 aaaatgtcag cagcagcgtg cgaatgtgcg agccgtccac gtcggcgtcg gtcatgatga
    10141 tgatacggtg gtagcgcagc ctctctggcg cgtactcttc tccgatcccc gtcccgagca
    10201 cgctgatgag cgtgatgatc tcctgagagg aaagcacctt atcgacacgc gccttctcga
    10261 cgttcaggat cttgcccttc agcggcagga ccgcctggaa tttgcggtcg cggccctgct
    10321 tggccgagcc gccggcggaa tcgccctcca cgatgtagag ctccgactgg gcggggtcgc
    10381 gctcctggca gtcggcgagc ttgcccgaga gaccgaagga atcgagcact cccttgcgcc
    10441 gcgtgagctc acgcgccttg cgcgccgcct cccgcgcgcg cgcggcatcc acgatcttgg
    10501 aacagataat gcgcgcgtca ccaggcttct cgaggagaaa ctcccccagc ttctgtgcca
    10561 cgatctcatc gacaaccggg cgcacttcgg aggacaccag cttgtccttg gtctgcgagg
    10621 agaacttcgg ctccggcacc ttcactgaaa gcaccgcggc caacccttct ttcatgtcct
    10681 cgccgctggt taccaccttc gccttatcgg cgagcttgct ggactcgata tactgattga
    10741 gcgtgcgcgt catcgcagcg cgcagcccgg tgaggtgggt gccgccgtcc cgctgcggga
    10801 tgttgttggt gaaacactgc acctcatcct gataggagtc gttccattgc atcgccacct
    10861 ctacgacgat gccgtccttc tccaccttgg agtagaaaat gttcgggtgc agcacattct
    10921 tgctgcggtt gatgtactcg acgaaacctt tcacgccgcc ggtgaacgcg aacgtttctt
    10981 ccttggcagt gcgctggtcg atcaggagga tcttgagccc gctgttgaga aacgcgagtt
    11041 cgcgcaggcg cttcgccagg atctcatagt ggaaatcgac cttgccgaag atctccttac
    11101 tcggcagaaa atggacttcg gtgccgcgtt tctcggtctt gccggtcgcc ttgaggggcg
    11161 caaccgtctc ccccatccgg aattccatct gatgcacctt gccgtcgcgg cggatggtga
    11221 gccgcagcca ttcggaaagc gcattgacga ccgacacgcc caccccgtgc agcccgccgg
    11281 ataccttgta tgaattgttg tcgaacttgc cgccggcatg gagctcagtc atcgccacct
    11341 ccgcgctcga gcggccctcc tcgtcttccg gatgcctctc ggtcggtatg ccgcggccgt
    11401 tgtctaccac gctgatcgag ttgtccgggt gtatggtgac cgtgatctcg ttgcagtaac
    11461 cggcgagcgc ctcgtccacc gagttgtcca ccacctcaaa catcatgtgg tgcagaccgg
    11521 tgccgtcgct ggtgtcgccg atatacatgc cggggcgctt gcgcaccgcc tcaaggccct
    11581 tgaggacctt gatgctgctg gcatcgtagt catcgggtgt acgttgctgc tcgtactcgt
    11641 ctgtctgtat cgccatccag cgtcccttag atccgcattg gcatcaccac atagcggaac
    11701 tcgcggttgt ccggcacggt gacgagcatg ctgctgttgg cgtccccgaa cgcgcattcg
    11761 acgctctcac tttgcacgtt gttcaacacg tccagcaggt aggtgatgtt gaaaccgatg
    11821 tcgagcgctt cacctccgta ggcgatttcc agctcctcct gggcttcctc ctgctcgttg
    11881 ttggtacacg agatgcgcag gctgttcgac cctatcatcc agcgcacacc gcggaatttc
    11941 tcgttcgaca ggattcccgc ccgctgcagc gattgcagga gctcctgacg tttgatagtg
    12001 aaatgcttct gatagttcgt cggcaccacc cgggtgtagt ccgggaactt gccgtcgatg
    12061 atcttggtcg tgagcacgac atcggcaaac gagaagcgca ccatgctggg gaagagctcg
    12121 acgacaaccg cctgctccgc gtccccgagt agcctcccca attccagcac cgccttgcgc
    12181 ggcagtatga cttcgcgttt ttcctgctgc tgtcccagcg gccgcgccgc gtaactcaga
    12241 cggtgcccat cggtggcgac gactttaagc tgtccctcat ccaacaccag cagcaggccg
    12301 ttcaggtaat agcgtatgtc ctgctgcgcc atggcatatt gcaccagcag cagcagttcc
    12361 cgcagcgcgc tttgcggcat cgtcaccttg gcctgcgccg ctcccgggtc ggccattgcc
    12421 ggaaaatccg ccgctggcaa ggtttgcaga ttgaaacggc ttttccccga gcgcacctgc
    12481 aggcggttgt tctgtacgtc cagcgtcgcc tcggtcgcct ccggtagcgc acgcaggatg
    12541 tcctgcagtt tccgggcgga cacagtcaag ctcacatcct ccccggttcc cttgccggta
    12601 tccgtgctgg tggccacctg gatctccagg tcagtcgcca ccagcagcag gcggccttgc
    12661 ttgcgctcga tcaggacgtt cgacaggatc ggcagcgtgt ggcgcttttc cacgatgccg
    12721 gttaccgcct gcaggggctt gagcaatgca tcgcgttcga gttgtacgag attcatagat
    12781 aaggatctat attaaaagta acaaccctaa tagtaccgtt cattcgctgt gtgcaactcg
    12841 ctatggagcc gagcacgagc gccagaacgg gaaggaaaaa ggagtgagaa agccggtata
    12901 agttgtggga aatttgtgga cgaaaagctc ttgattgaag ccgatgctgg atatccacaa
    12961 agtacacagg aaaaatcaac ttctcagcac gttgagaagc gagttgaaat cgcgggtgag
    13021 ctcgctgttg gtcgacttga gcagggcgac cttgcgacag gcatggagca cggtcgtgtg
    13081 atcacggccg ccgaatgctt cgccgatgtc cggcaagctc aactgcgtca gttccttggc
    13141 gagcgccatg gcgatctggc gcggacgggc gacattgcgg ctgcgcttct tcgagtacat
    13201 ctccgcgacc ttgatcttgt agtaatcggc gactgttttc tggatgtttt cgattgaaac
    13261 ttggcggtgc tgtatggcca ggagatcgcg cagcgcctcg cggcacaact cgaccgacag
    13321 ctcgcggccg gagaaacgcg cgtaggccag cacacgtttg agacctcctt ccagctcgcg
    13381 cacgttggac tggatatgct tggcgaggaa aaaggctact gcctcgtcca gcctgacccc
    13441 ttcggcttcc gctttcttga gcaggatggc aacacgcatc tccagttccg ggggttcgac
    13501 ggcgacggtc aggccccagc caaaccggga aatgaggcgg ttttccatgc cggcgatctc
    13561 cttgggatag gtatcgcagg taatcaccac ctgcttatgc gcctcgacca atgcgttgaa
    13621 tgcatagaaa aattcctcct gggtgcggct cttgccgctg aagaactgga tgtcgtcgat
    13681 cagcagcagg tccagtgaat ggtaatagcg cttgaagtca tcgaatgcct tgtgctgata
    13741 ggcgcggacc acatccgata cgtattgctc ggcatggata tagcgaatct tggtctcggg
    13801 tgcctgttca cgcagtcggt tgccgatcgc ctggatcaag tgcgtcttgc ccagccccac
    13861 gccgccatat atgaacagtg ggttgtaggc cgcgcccggc cgctccgcga cctgggctgc
    13921 agcagcccgt gccagttcat tggccttgcc ggtgacgaaa gtctcgaagg caaatgccgg
    13981 gttaagccgg gatacttcgc ggctgctcgg cttcgctccc cggtctttct gggcctgctg
    14041 acgagggaga tgcaagggcg gcgccggggc gatttccttg tcccccaggg tgaggagcac
    14101 ttctgcattg cgcttgagct gctcgcgcgc gagcagctcg atgcgaggga gaaacttgtc
    14161 gcgtatccac tgcagcacaa aacggttggg cgcgaccagc gtgagccggt cgccatcggc
    14221 gtgaaatcgc aggggcttga tccatgtttg gtactgctgg ggtggcagct ccttctcgaa
    14281 gtaggtcaga cagaagcgcc agaaactgtt catcggcgtc gcgttggagt tggaatttgg
    14341 acccggaaga aggcgagttt tcaggctgag attttagcct ttttgcggcc ggttatccac
    14401 aaaatccgcg cttccgccgg atggtttgaa cgaccaagaa aaggttgaca tggcggagag
    14461 ataaaggttg taatagcgcg cttttcagca gccgggcaga ccaagatgaa acggacctat
    14521 cagccatcgg tgacgcgccg caagcgcact catggttttc gcgcgcgtat gcggacccga
    14581 ggcgggcgtg cggtgctgcg cgcccgtcgt gccaaaggac gcgcgcgatt gagcgtctga
    14641 gcaccaggac agctgccaca ctgccgcgca gcgcccggct gctgaaacgt gagcagtata
    14701 gggccgcgct ggcagctggc gcggccaaaa tacgccgcca tttcaccttg tacgtcaggc
    14761 ccaatggcct gtctctagct cgcataggca tcattgccag caaacgggta gcgtcgcggg
    14821 cgatcgaccg caaccgggct aaacgcctgg tgcgggaggc attccgcaag gcgcgccatc
    14881 gcttgggggg cgtggacgtg gtggtcgaat tgcgccgttg tccggcccgg gagttcagca
    14941 tggcggcggg cgcggagatc gccaggctgc tggaagagat aggcacactg tcgcactgcg
    15001 gctagtgccg ttcacctacg gccatggatt cgcaacgcct gattcttttt ttcgttttct
    15061 cgttctcggt gttcctgttg ctggatgcct ggcaacgcga tcagccgccg cctcagccca
    15121 cggcgccggt cagcgacaag accggtaaac agccgccggc tgctgtacaa ccgccggtcc
    15181 cgaccggcaa acccgccatc ccccagcctg cagtgccgga gcaggagcgg cgcgccccgg
    15241 aagcaagcac cgcgatacaa gtcgagaccg acttgtacgt cgcgcagatc aacactcgag
    15301 gcggagacct gagccggctg cagttcagca agcaccgtga cacactcgac aggcgcaagg
    15361 acttcgtgct gttcgagagc actgtggagc gtacctatgt tgctcagtcc ggcctgattg
    15421 gcagcggcct gcccaaccac cgcacggcgt atgcggctgc cggcaacagc taccagctgg
    15481 ccgaaggaca ggacagcgtg gaagttcggc tgaccgcccc cgcagccgac ggcgtgcagg
    15541 tgaccaaggt ttatcgcttc cgtcgtggaa gctacttgat cgacgtgggt ttcgagatca
    15601 acaacgggag cgcggtggcg atgcagccgc acgcctattt tcagatggtg cgcgacggca
    15661 agccgcccgc tggggactcg gcaatgttgc cgacgttcac cggagtggcg gtctataccg
    15721 acaaagagaa attcaagaaa ttgccgttcg ccgacatcga caagggcaaa gtcccgtacc
    15781 ccaagcacgg caacgacggc tggatcgcca tgctccagca ttatttcttc agcgcgtggc
    15841 tgcccaagaa cgggacggcg cgcgagtttt acacgcgccg gctggatgaa aacctgtatt
    15901 cggcaggcgt gatcgttccg gtcggccttg tcaatgccgg cgccagcgca gcgatcgccg
    15961 ttccgctcta tgccggaccc gaggagcagg aaaagcttgc aaagatcgca ccaggtctgg
    16021 atctcacgat cgattacggc tggctgacgg tgatcgccgt gccgctgttc tgggtgctcg
    16081 cctggttgaa acagtgggtg ggcaattggg gcgtcgccat cattctgctc acggtgatca
    16141 tcaaattact gttctacccg ctgtcggcgg cgagctaccg ctcgatggcc aagatgaggg
    16201 tgctggcccc caagctgcaa cggctcaagg atcagtacgg tgacgaccgc cagcgcctgc
    16261 aacaagcgat gatggagctc tacaagaccg agaaaataaa cccacttggc gggtgcatgc
    16321 cgatagtggt acaaatacct gtttttattg cgctttactg ggttttgctg gcgagcgtgg
    16381 aactgcggca cgcaccattt acgctgtgga tagacgacct ctcggcgccg gatccgtggt
    16441 tcattctgcc gatactgatg gcgctcacca tgttcgtgca aacctggctc aacccggagc
    16501 caccagaccc cgtgcaagcg cgggtgatga agatcatgcc gatcgtattt agtatcttct
    16561 ttttcttctt tcccgcaggc ttggtgctgt attggctggt gaacaatgtg ctctcgatcg
    16621 cgcagcagtg gagaatcacg cgcgtattgg agcgcgaggc cgcgccacgc aagaattgac
    16681 ggcacagcag cgttcgcggc ttgcgaccga gccgctgcag gctgatgacc gagaacgaca
    16741 ccattgccgc cattgcaacc gctcacgggt tcagcggcat cggcgtggta cgggtgagtg
    16801 gtccacgact ggattccgtc atagcggatc tcgtcggcaa gccgttgccg ccacgtcatg
    16861 ccgtgctggc cgaattccac gatacgcgcg gcgaagtcat agatcagggc atcgcgctct
    16921 acttcccggg gccgcgttcg tacaccggtg aagacgtgct ggagctgcag gggcacggag
    16981 gccctgtagt gctgcaacag ctgctcaaac gctgtcttga gctgggtgcg cggcctgcac
    17041 ggcccggcga attcacttta cgcgcctatc tcaacgataa gctcgatctc gcgcaagcgg
    17101 aaagcgtggc ggatctcatc aacgctgcga cggcagaggc agcccgcggc gcattgcgct
    17161 ccctccaggg cgtgttctcc cagaagatcg aagaactcgt gcgtagactc gttgagctgc
    17221 gggcactggt tgaagcgaca ctggatttcc ccgaggaaga aattgaattc ctgaaacaga
    17281 tcgatggcga aggcaggctc gcaatcattc gcgccaagct gcaggatttg ttagcagcct
    17341 cgcaacaggg cagcctgctc cgggagggaa tccacatagt gctggcgggc cagcccaatg
    17401 tcggcaagtc gagcctgctc aaccggctgg ctggagaaga actcgccatc gttaccgagg
    17461 ttcctggaac cacacgcgat gcaatacgcc agagcatcaa cctggaggga atcgcggcgc
    17521 acatcatcga cacggccggt ttgcgcgatt caagcgatcc cgtggaacgg atcgggatcg
    17581 agcgcacctg ggctgcgatc gagaaagccg atctggtgtt gctgttgatc gacgcgactc
    17641 aaggtgaaag caatgcagac agaaacatcc tgaatagact tccatccaag ctgccttgca
    17701 tcagagtgat gaacaagatt gacttgctga cgggcccagc gtcactcgag cggcgcgacg
    17761 gtcgggatcg ggtatggctc tcggccaaga ccggggccgg aatcgatttg ctgcgcaaca
    17821 ctctgttgga aatggtgggc tggcaaggca ggggagaggg attattcatg gcccgcgagc
    17881 gccatgttca tgctctaaag acagcccagt ttcatttgga aagcgcagcc cagcaagggc
    17941 gacagttgga gctgtttgct gaggagttgc ggctggcgca ggaatgcctc gggaccatca
    18001 cgggtgagtt cacagcggat gatttgctag gggagatatt tagccagttt tgcatcggta
    18061 agtgagctac caacaaggtg tttcacgtgg aacattgtcg aatactgatg atgctgcact
    18121 gcaacatcat tgagtttcac gtggaacata cgaacccctt cgtattctgg tattcgtgaa
    18181 tttaatgtat tattgcaagt gtccgaatca ggatcgaacg tatcgatgca agccattgaa
    18241 aaatatgatg ttattgtggt cggagggggc cacgccggaa ccgaagcagc ccttgccacc
    18301 gcgcgcatgg gttgtgaaac actgctgctc acccacagca tcgaaacctt gggtcagatg
    18361 tcatgcaacc cttcaatagg cggcatcggt aaaggccacc tcgtcaagga gatcgacgca
    18421 ctggacggag cgatggcggc ggccaccgac gaggccggca tccagtttcg cattcttaat
    18481 tcgcgcaaag gtcctgccgt gcgggccacg cgcgcccagg ccgatcgcct gctctacaag
    18541 caggcgatac gcggacgcct tgaaaaccag caccgattgc gcatcttcca gcaggcggtt
    18601 gacgacctgc tgctcgaagg cgaccgcgtc acgggcgtag tgacgcagat cgggctcaag
    18661 ttttccgcgc gcgccgtcgt gctcacgacc ggaacctttc tcgccggatt gattcacgtc
    18721 ggcctgaaaa attaccaggc aggccgcgcg ggtgatccgc ccgcgctcac gctcgcgcag
    18781 cgtctgcgcg agctgaagct tcccgtggga agactgaaga ccgggacccc gccgcgcatt
    18841 gacgggcgca gcatcgattt ttctgtgatg gcggaacaac cgggcgacga gccgacgccg
    18901 gtgttttcct ttctcgggcg ccgcgagcag cacccgccgc agctcccgtg ctggatcacg
    18961 cacaccaacg cgcgtaccca cgaaatcatc cggaatgcgc tcgatcgctc gccgctctac
    19021 accggcgtga tcgaaggcgt cggcccgcgc tactgtccat cgatcgagga caaggtgatg
    19081 cgctttgcct cgaaggcctc gcatcagatc tttctcgaac ccgaagggtt tacggtcaac
    19141 gagttctatc ccaacggcat ctcgaccagc ctgccgtatg acgcacagca ggaactggtg
    19201 cgttcgatca ggggccttga gcgggcgcac atcacgcgcc cgggctacgc catcgaatat
    19261 gactatttcg atccgcgcgg cctcaagcgc tcgcttgaga ccaaggccat ttccggcctt
    19321 tttttcgcgg gacagatcaa cggcaccacg ggatacgaag aagccgccgc gcaggggata
    19381 ctcgcaggca tcaacgcggc gcttttcgta aaggaacacg agccttggtg cccgcggcga
    19441 aatgaagcct atcttggcgt gctggtggac gatctcgtca cgcgtggcgt gtccgaaccg
    19501 tatcgcatgt tcacgagccg cgcggagtat cggctatccc tgcgcgagga caatgccgat
    19561 ttgcggttga ctgaaacagg gcgccgtctg ggcgtggtgg gcgacgcgcg ctggggagca
    19621 ttttcgcgca agcgcgaggc gatcgcgcgc gaacaagagc gcctgaagag cacctggatc
    19681 aacccccggc tgctgccgcc ggaaacgctg gcctcggtct tcatccaacc gattggacgt
    19741 gagcagccac tcaccgagct tctgcgccgc cctgatgtga gctatgcgca gctgatggag
    19801 ctgcccggcg ccggtcccgg cgtgtccgat ccgcaggtgg ccgagcaggt tgaaatccag
    19861 gcgaaatacc agggctatat cgagcgccag agcgaggaga tcgcgcgtca tcagcaccat
    19921 gagaacctgc gcctgccggc aggggtggac tatcgcgagg tgcgcgggct ctcggttgaa
    19981 gcgcagcaaa aactgaacga gcaccgccct gaaacgcttg gccaggcgtc gcgcatctcc
    20041 ggcattactc ctgcggcgat ttccgtgctg ttggtgtatc tgaagcggag gttagcgggc
    20101 actcacttcg gtaaaccggc gaacccggga gctagggggc gcactgcaac taagacagcg
    20161 tgacataagc cttgcggcgc aactcgccga aggcgtcgcg caactcgatc tcgccctgcc
    20221 gccacaggcc cagtcgcgac tgctggatta cctggggctg ctgcgaaaat ggaaccggat
    20281 ctacaacctg accgcggtgc gcgaagcctc gagaatggtg agtcatcacc tgctcgattg
    20341 cctggcggtg gtgccgcatg tggcggcgag cacgatcctc gacgtcggca gcggcgcagg
    20401 gttgcccgga ataccgctcg cgctcgcgct gccgcgggcc cacgtgacgc tgctcgagtc
    20461 cagccacaag aagacggctt ttctccagca ggcgaagttc gagctgaagc tcgacaacgt
    20521 cgcggtggtc tgcgagcggt tggaattatg gcggcccggc cgggcgtttg agctggtgat
    20581 ctcccgcgcc ttctcggatc tcgccgaatt cgccgcctta gcggggcgac acgtcgttcc
    20641 cggcggcagg ctggcagcga tgaagggcgt ctatccgcac gaggaactcg ccggtccgct
    20701 gcacggctgg cgtttgcaga aggcgattcc gctaacggtg cccggcttgc gcgcccagcg
    20761 ccatttgctg ctgctcgagc gggaatgacc gtgccaagaa tattggccat caccaaccag
    20821 aaaggcggcg tcggcaagac caccaccggg gtcaatctag ccgcgagcct cgccgcagcg
    20881 cgccggcggg tgctgctggt ggacatcgat ccccaaggca acgccaccat gggcagcggc
    20941 gtggacaagc gcgccctcgg cgctacggtc taccacgtgc tgctgggcac gcggcgcatc
    21001 gcggaagtgc gggcgcgctc ggaaaccggc gagtatgacc tggtgccggc caaccgcgag
    21061 ctggctggcg ccgaagtcga gttggtggaa gtggagcatc gcgagacgcg cttgaaggac
    21121 gcgatcgcca gcgttgcgga cgagtacgat ttcatcctga tcgactgtcc gccggcgttg
    21181 aacctcctca cggtcaacgg gctgaccgcg gcccaggcgg tgattatccc gatgcagtgc
    21241 gagtactacg cgctggaagg cttgagcgac ctcgtgcaga ccatcaagaa agtacgcgct
    21301 cacctcaatc cgggactgga aatcgaaggc ttgctgcgca ccatgtacga ctcgcgcaac
    21361 acgcttgcgc agcaggtctc ggatcagctg cttgagcatt tcggcgacaa ggtctatcgc
    21421 accgtgattc ctcgcaacgt gcggctggcg gaggctccga gcttcggggt gcccgcgctc
    21481 aagttcgaca agaattccaa aggcgcgcag gcctaccttg cccttgcggg ggagatgttg
    21541 aaccgggcgg aaggatgagg agcgagacga tggcaaaaat caaggggctg ggacgaggac
    21601 tggacgcgct gctgggctcg gtcgaggaac catcggccag cgagacgctc accacgctcg
    21661 cggtcgaggc attgcagccg ggacgctacc agccgcgcac gcgcatggat ccggaagcgc
    21721 ttgccgaact cgccgaatcg atcaaggcgc agggcgtgat gcagccgata ctcgcacgcc
    21781 cgatgggcgc gggccgctat gaaatcgtcg caggcgaacg ccgctggcgt gcagcgcgca
    21841 tggctgggct tgctaccgtg ccggcgctgg tgcgtgaggt gcccgaccgg cacgcgctcg
    21901 ccatcgcgct gatcgagaac ctccagcgcg aggacctgaa cccgctcgag gaagccgcgg
    21961 gcgtcaagcg cctgatcgag gaattcggca tgacgcatgc ggaggccgcc gaaggcctcg
    22021 gtcgctcgcg cgctgcgatc accaatgcgc tgcggctgct tgagctcgcg ccgccggtgc
    22081 aggagctgat gcgcgaaggc aaactcgaca tggggcacgc gcgcgcgctg ctggcgctgc
    22141 cggcgttgaa gcagatcgag cttgcgcgcg aagcggcggc gaagcagctc tcggtgcggc
    22201 aggtcgagca gaaggtagcc cgcctcgcca ggcgtgctac ggttggcggg cgccccagaa
    22261 ccgatcgcga cgtcgcgcgg ctcgaggaag agtggagcca acggctcgga accacggtac
    22321 ggattgaggc cggtgctaag ccaggcaagg gtcgactggt gattcattat tcgaccctgg
    22381 atcagctcga cgcgctgctg cgcaagctcg gctagggcgg gttgcgaaat gccggcttgc
    22441 cgcgacgcag caaattgacc cgtttcgcgt cgcaaggcta tactggcggg gctttgctcg
    22501 gcgcggataa aaccaacgcc atgcttcgcg cactgggcaa gccgatacgc accgttctcc
    22561 ggtggcaact gctggccacc gccgcattga tgctcgttgc cggagcctgg gctggcgccc
    22621 acggcgctct gtccgcggct ctcggcggct cggtcagcat cttctccgga ttcacgtctg
    22681 cgatcgtggc cgcgttgggc aaggcggagt ccgcaggggg ggtggtcgtg actgcgctca
    22741 cggctgaagc cgttaagatc ggcctgatcg tgatgctcct gtggctcgtg ttggcgaact
    22801 acgacggcgt cgtggtgcac gccttcatcg ggtcattcct ggtgacggtc ctgattttcg
    22861 ccatggcgtt ctttgtgcgc gaatactaga acgagagcgt tcgagcgatg gctgccggca
    22921 aagaactcaa tccctccgac tacatccagc atcacctcac gttcttccgt gcgccgctgg
    22981 gcgaaggcga gttctgggcg ctcaacatcg acaccgtcgt cacgtccgta ttgctcgggc
    23041 tcgtcggcct cggtttcatc tggtgggtgg tgcgcggcgc gaccccgggg gtgccggaca
    23101 agcgccaggc gttcgtcgag ctgctgatcg agttcgtcga tgaccaggtg aagggcattt
    23161 tccaccacgg cgaccgccac cggttcatcg cgcccgccgc cctcacggtg ttcgtctggg
    23221 tggtgttgat gaacgcgatg gattttctgc cggtggactg ggttgccggc gctctcggca
    23281 tcgtcggcct gcacgaatgg cgtccggtgc ccaccgccga cgtgaacgcc accttcgcgc
    23341 tgtcggtctc ggtgctcctg ctgtcgatct atttcgcggt cgccgtgaag ggcctcggcg
    23401 gctggatcca cgagctgttc tgcgccccgt tcggcgcgaa cccgttgctg tggccggcga
    23461 acctgctgtt caatttggtc gagtacgtct cgaagacgct ttcacatgcg atgcggctgt
    23521 tcggcaacat ttacgccggc gagatcctgt tcttcctgct ctggctgtgg gccgcgaccg
    23581 gcctggtcgg atccttcttc ggattcctgt tcggcctggg ctgggcgatc ttccatatcc
    23641 tgatcgtcct gctgcaggcc tatatcttca tgatgctgac catcgtctac atctcgatgg
    23701 cgcacgagca ccactgattc cccaaaacaa ccgtctgttc gctcaggaaa ggaaaacacc
    23761 atggaaaaac ttcagttgct ggcgatggtc caggcctaca ccagtatcgg catcggcctc
    23821 atggtcggcc tcggcgcggc cggggcgtgc atcggcattg gcatcatgtc gagcaagttc
    23881 ctggaggccg cagcgcgcca gcccgagctc accaactcgc tacagggcag ggtgtttctg
    23941 ctgctcggcc tgatcgacgc ctctttcatc atcgggctcg gcatcgcgat gttcttcgcg
    24001 tttgccagcc cgctggcgac gccgttccgc tagagcgcgg cgcgcgctgc accccgaggg
    24061 gaaacgtatg agcttcaatc tgacgctgat cacccagatg ctcgcctttg cggcgttcat
    24121 cctgttcacc gtgaaggtcg tctggcccta cctgttgcgc gccatcgagg aacgccagaa
    24181 gacgatcgcg gacgggcttg ccgctgcgga acagggcaag cgcacgctgg agatttcgac
    24241 ccggcaggcc gaggaggcga tcaagcaggc gcgcgcgcgg accgccgaga tcatcgcgca
    24301 ggcggagaaa cgcgccgcgc agatgatcga cgaggcacgc atgcatgcga aggaggaagg
    24361 caatcgcgaa aaagtggcgg cacaagccga gatcgagcag caggtcacgc gcgccaagga
    24421 agcgctgcgc gaacaggtcg cggcgctcgc cgtggccggg gcggagagga tcctgcgccg
    24481 tgaggtggac gcgaagacgc acgccaacct gctcgcacgg ctcaaacagg aaatgcgtta
    24541 aggtcatggc tgaaaccgtc accatcgcgc gtccctacgc cgaggccgta tccgcgctcg
    24601 ccgatgcggg cggcgcgctc ggcaaatggt cgcagatgct ggcggtgatg gcgacggtgg
    24661 cggcgacacc cgagatgaaa gccgcgaccg gcgaccccaa tctcacctcc gatcagatct
    24721 acggactgtt cgccggggcg tgcggcgacc tcacgacgga agcgcagaac ttcgtgcgcg
    24781 tgctgatcga gaacgaccgg ctcgcggccc tgcccgagat ccgcgacata ttcaaggaac
    24841 tcaagaacga gcgcgagggc gtggtggacg cggtgatcac gaccgcctat ccactcgacg
    24901 gcggccagct cgccgcgctg gtggcggaac tggaaggccg gttcaagcgc aaggtcaatc
    24961 cccaggtcaa tgttgacccg gagctgatcg gcggcgcgca cgtgcaggtc ggcgacgagg
    25021 tcatagacgg ttcggtgcgc ggcaagctcg ccgagatggc ggccgcgctg atgaaatagg
    25081 caaccaagga aagcgtatgc aacagctcaa accctccgag atcagcgatc tgatccgctc
    25141 caggatccag agcctggcgc tcgccgcgga tatccgcacc cagggcacgg tggtctccgt
    25201 gaccgacggc atctgccgcg tccacggcct ttccgaggtg atgcaaggcg agatgctgga
    25261 atttccgaag aacaccttcg gactggcgct caacctcgag cgcgactcgg tcggcgcggt
    25321 gatcatgggc gagtacgagc acatcaccga gggcgacacg gtgaaatgca ccggcaagat
    25381 cctggcggtg ccggtcggcc cggagctgct cgggcgcgtc gtgaactcgc tcggccagcc
    25441 gatcgacggc aagggtcccg ttcccgccaa gatgaccgac gtcatcgaga aggtggcgcc
    25501 cggggtaatc tggcgcaaat cggtctcgca gccggtgcag acgggcttga aggcgatcga
    25561 ctcgatggta ccgatcgggc gcggccagcg cgagctcatc atcggcgacc gccagaccgg
    25621 caagaccgcc gtggcggtgg acacgatcat caaccagaag ggcaaggagc tcatttgcgt
    25681 ctacgtggcg atcggccaga aggcctccac catcgccaac gtcgtgcgaa agctcgagga
    25741 gcacggcgcg atggcctaca ccatcgtggt ggcggcttcc gcctcggaat cagcggcgat
    25801 gcagtacatc gcgccctact ccggctgcac gatgggcgaa tatttccgcg accgcggaca
    25861 ggacgcgctc atcatctacg atgacctcac caagcaagcc tgggcctatc gccagatttc
    25921 gctcctgctg cgccgcccgc cgggccgcga ggcgtacccg ggcgacgttt tctacctgca
    25981 ttcacgcctg ctcgagcgcg ccgcccgcgt gaacgaacag tacgtggaga atttcaccaa
    26041 gggggcggtg aaaggcagga ccggctcgct gacggcgctg ccggtgatcg aaacgcaggc
    26101 gggtgacgtc accgcattcg tcccgacgaa cgtcatctcg atcaccgacg gccagatctt
    26161 cctcgagacc gaccttttca acgccggcat ccggcccgcg atcaacgccg gcatctcggt
    26221 gtcgcgggtg gggggcgcgg cgcagaccaa gatcatgaag cggcgcgaca cgggaggcgg
    26281 ggtgcggctt gcgctcgccc agtatcgcga gctcgccgcc ttcgcccagt ttgcctccga
    26341 cctcgacgag gcgacccgca agcagctcga acgcgggcgc atggtgaccg agctcatgaa
    26401 gcaaccgcag taccagccgc tgcaggtgtg ggagatggcg ctgacgttgt tcgcggtgaa
    26461 caacggctac ttcgatgaca tcgacgtcaa gaaggcgctc tccgccgagc gctcgatgcg
    26521 cgactacgtc aaggacaact acagcgacct gatgaagcgc atggaggacg tgaaggactt
    26581 gtcgaaggac gacgagcaga agctgcacga ggcgatcaag gattggaaaa aaaacggcag
    26641 tttctagacg ctgacgcgct gaaactgctg cgcgcgttgg aacggacgaa tagatgatca
    26701 ggaaaacagt tcacgaaatc tgctcgccgc tcaacgcgcg cgggagacgg tagatgccgg
    26761 gcaccaaaga aatcaggatg aagatccgga gtgtgcagaa cacccggaag atcaccaagg
    26821 cgatggagat ggtcgcggcc tccaagatga ggcgcgccca ggaacgcatg cgcgcggcgc
    26881 gcccctacgg cgagaagatc cgcaacgtcg ccgcgcacat cagccatgcc aatcccgagt
    26941 accgccatcc cttcctgatt tcgcgggaca cggtcaagcg ggtcggcatc atcctcatca
    27001 ccaccgacaa gggcctgtgc ggggggctga acaccaacgt gcagcgcctc gcgctctcca
    27061 ggatcaagga atgggaggcg cagggcgaga gcctcgaggt gtgctgcatc ggcaacaagg
    27121 ggctcggctt catgcagcgc ctgggcgcca atatcgtgtc gcaggtgacg cagctcggcg
    27181 accggccgca gatggaaaag ctgatcggca cggtgaaggt gatgctcgac ggctacatcc
    27241 aggaccgctt cgaccggctg atgattttct acacccg
//
LOCUS       DBNGWZ010000632         7554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1366430, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000632 DBNGWZ010000000
VERSION     DBNGWZ010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1366430"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="AC16CX_19840"
     CDS             <1..1180
                     /locus_tag="AC16CX_19840"
                     /inference="COORDINATES: protein motif:HMM:NF012975.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR3014796.1"
                     /translation="AFPDLPAPAVPQDLPLAEVEAFSIDDVTTTEIDDAFSVTMRPGG
                     KLRIGIHLAAPALGITPGSAIDTVARERLSTVYFPGGKITMLPEAAIGAYTLSERRQC
                     PALSLYLEVAPDGSVAGRTTRIERVHIAENLRHYVLEETFNEDTLAAGRVEHRYGEAL
                     TALLRFARGLEEARRGESAEYEPRPEYSFHVENDRVRIVRRRRGTPIDKIVSELMIQA
                     NSAWGRELAANGVAGIYRVQDGGKVRMSTVPAGHEGLGVDSYAWASSPLRRYVDLVNQ
                     RQLIALARGEAAPYRANEERLLAAMRDFESAYDAYGEFQRQMERYWCLRWLLQEGVST
                     VTATVIRESLARFDELPLVVRVPSLPVLDPGVRVELAIADLDLLDLTLRCEFRRQLAP
                     "
     gene            complement(1186..1740)
                     /locus_tag="AC16CX_19845"
     CDS             complement(1186..1740)
                     /locus_tag="AC16CX_19845"
                     /inference="COORDINATES: protein motif:HMM:NF014912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3014797.1"
                     /translation="MRKVAKKPRRARGAREPGIESVAELMTYAYAMETEAAERYAEFA
                     DAMEAHNNAEVAELFRKLARIEHQHGEQVLQAMGWAAPPPLHGDFRWQGTEAPESGER
                     GELHYLMHPYHVLKIAHQNEQRAFEFFSRVARSAGNAALRRAAAEIEEEERQHVRLIE
                     EWLKRTPQPPSDWALDPDPPAGVG"
     gene            complement(1788..2633)
                     /locus_tag="AC16CX_19850"
     CDS             complement(1788..2633)
                     /locus_tag="AC16CX_19850"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3014798.1"
                     /translation="MNRPVPVATGEAPAAKDFKSGYKPIWCPGCGDFSVLSSLTKALA
                     MLGLRPENVAVVSGIGCSSRIPAYTTCYGFHGVHGRALAAAAGLKLARPDLTVVVTGG
                     DGDGYSIGGNHFLHACRRNVDLTYVVMDNHVYGMTKGQPSPTTEPDWDSKLSPGGTGL
                     RTFHPLVIALAAGANFVARAFSGDPGGTAEIIAQAIRTPGFSFVEVLSPCVTYRPEQR
                     EWKKAVRPAPVATTDDPVRAARRIMTDDGFYIGILYSGTRQPFQPPIGIGEATVADLE
                     RDFAL"
     gene            complement(2630..4372)
                     /locus_tag="AC16CX_19855"
     CDS             complement(2630..4372)
                     /locus_tag="AC16CX_19855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3014799.1"
                     /translation="MAHTVAVSKAARSLSLAIAGSGGAGVMTAGNMLLEAAARAGWYG
                     LMVRISGPQIRGGEAAALLRFAPQPVESLDDHFDVLIALDWQGVHRLASEIPLGPGSL
                     IIVDPAQGAPPESYVKGAARCVELPMKNLSKSVPGSWPNMIALGIAAGVLGLPAGAVD
                     AVVEKSLNGTAHAAVAASLAAVAAGRAAASDIGASFMLARPVQSGPRWLITGNEAAGL
                     GAIRGGVRFVAGYPITPATEMLEWMAPALAQVGGVLVQAEDELASINMAIGASYGGVP
                     ALTATAGPGLSLMTEALGLAVAAEVPVVVVDVMRGGPSTGIPAKTEQSDIAIAVNGLH
                     GDAPRIVLAPNSIADCIVTTQWAVHLAESLQVPALVLSDQFMGQSRAVIDASPAVSFA
                     GARETAAAGAANYQRYADSASGVSPMAIPGTAGIAYTADGLEHDESGVPSSEAADHYV
                     QLAKRSRKLAQANYGPLWADVEGDGEIAVITLGSSTGPVREALAAARARGVSARMISI
                     RLLAPARPAEFARALDGARRVLVVEQNGGAQLYRYLRSQYDLPGEVKSLARPGPLALR
                     PSEVGRVLAEWDHA"
     gene            4513..5625
                     /gene="oah"
                     /locus_tag="AC16CX_19860"
     CDS             4513..5625
                     /gene="oah"
                     /locus_tag="AC16CX_19860"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HIR3014800.1"
                     /translation="MPGDADLKNHDLTEGVVVPGVRVERRPAKTPDGKHAAGLYNAWI
                     WIDNPAQYNSYTTDIVKGLIHAFRSASNARDVVCTVFTAVGDKAFCTGGNTKEYAEYY
                     AGRPQEYRQYMRLFNDMVSSILACDKPVICRVNGMRIGGGQELGMACDFSIAQDLARF
                     GQAGPKHGSAPVGGATDFLPPLIGADRAMVACVMCEPFSAHKAYHWGMLADIAPALKV
                     DGRFIANPLVETQRFADEYGRIVFGDFKTGEAARQGKALMARGQVDLSMLDARVEDMC
                     ARLLLTFPECTTKTIEELRKPKLEAWNRNKENHRAWLGLNMVTEGRAGFRAFNEGPKD
                     DREVDFIALRQALARGEGWSDALTERIQPGRKKADA"
     gene            5622..6392
                     /locus_tag="AC16CX_19865"
     CDS             5622..6392
                     /locus_tag="AC16CX_19865"
                     /EC_number="4.2.1.100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexa-1,5-dienecarbonyl-CoA hydratase"
                     /protein_id="HIR3014801.1"
                     /translation="MNDAPLRVWLDRGGALLRLRLSRPQANIIDGPMVAALRAAFGEH
                     RATAGLRGALLDAEGPHFSFGASVEEHLPERCAGLLAALHDLIGEMFDWPAPILTAVQ
                     GQCLGGGLELACAGSLIFAAADAKLGQPEIRLGVFAPAASCLLPERIGQAAAEELLLS
                     GRSISAQEALALGLATAIAEDAQAAALDWFDRHLADKSASSLALALAAARAARGAGLR
                     RRLRKIEALYLDRLMKTRDANEGLRAFLAKRAPKWEHA"
     gene            6392..>7554
                     /gene="bcrC"
                     /locus_tag="AC16CX_19870"
     CDS             6392..>7554
                     /gene="bcrC"
                     /locus_tag="AC16CX_19870"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HIR3014802.1"
                     /translation="MTDSVAKIVERCQALYEDLNFEAVKRWKAAAPGRKAIGYMPLYV
                     PREIVHAAGMLPVGILGGGDQLEVIQGDAYYQSYICRIPRSTIELGLTGRLDCLDGML
                     FPSICDVIRNLSGMWQIMFRGKYVRYFDVPQNFDDAIGGEYYVHELEVLREDLARLAG
                     AEVGDAALRSSIALYNENRRVVRELYAYRAREPWQAPTSEVYLVLRAGMLLPVEEHTR
                     LIRDYLAATAAEPRPKRDNARVALTGAFCEQPPLALIKSIEMAGCYIVDDDFMLVLRW
                     LLDDVPESGDPLQNLAQSFLHRSVSTASKYEARREDKGRYLLQQVRNTAAEGVIFAAP
                     SFCDPALLDRPMLLDVLAQHGIPCTAFKYAENTGQMAPIREQAGTFADSIKLW"
BASE COUNT         1211 a         2587 c         2583 g         1173 t
ORIGIN      
        1 ggccttcccc gatttgcccg cgcctgccgt gccgcaggac ctgccgctcg cggaggtgga
       61 ggcattcagc atagacgacg tcaccaccac cgagatcgat gatgcgttct cggtgaccat
      121 gcgaccagga ggaaagctcc gcatcggaat ccacctcgcc gctcccgcgc tggggattac
      181 gccgggctcc gccatagaca ctgtcgcccg cgagcggttg tccaccgtgt acttcccggg
      241 cgggaagatc accatgctgc cggaggccgc gatcggggct tacacgctgt cggagcgcag
      301 gcagtgcccc gcgctctcgc tctacctgga ggttgcgccc gacgggtccg tcgcggggcg
      361 gacgacgcgc atcgagcggg tgcacatcgc ggaaaacctc cgccactatg tgctggagga
      421 gacgttcaac gaagatacgc tcgctgcagg ccgcgtcgag caccgctatg gggaggcgct
      481 cactgcactg ttgcgcttcg cgcgcgggct ggaggaggcg cggcgcggcg agagcgccga
      541 gtacgagccg cgccccgagt acagcttcca tgtcgagaac gaccgcgtgc gcatcgtccg
      601 gcgccgccgc ggcacgccga tcgacaaaat cgtctccgag ctgatgatcc aggcgaacag
      661 cgcgtggggg cgggagctgg cggcgaacgg cgtcgcgggc atctaccgcg tgcaggacgg
      721 cggcaaggtc aggatgagca cggtgccggc ggggcacgag gggctgggcg tggacagcta
      781 cgcctgggca agctcgccgc tgcggcgcta cgtggatctc gtgaaccagc gccagctcat
      841 cgcgctcgcc cggggcgaag cggccccgta ccgggcgaac gaagagcggc tccttgcggc
      901 gatgcgcgac ttcgagtccg cctacgacgc ctacggcgag ttccagcgcc agatggagcg
      961 ctactggtgc ctgcgctggc ttctccagga gggcgtttcc acggttacgg cgacggtgat
     1021 ccgagaaagc ctggcgcgtt tcgacgagct gccgctggtc gtgcgggtgc cctcgctgcc
     1081 ggtgctcgac ccgggcgtcc gcgtcgaact cgcgattgct gacctggacc tgctggatct
     1141 caccctccgc tgcgaattcc ggaggcagct cgcgccctga cgaagttacc ccacgcccgc
     1201 ggggggatcg gggtcgagcg cccagtcgga gggcggctgc ggcgtgcgtt tcagccattc
     1261 ctcgatgagg cgcacatgct ggcgttcctc ctcctcgatt tcggcagcgg ccctccgtag
     1321 cgcggcatta ccggcgctgc gcgcgacgcg gctgaaaaat tcgaaggcac gctgttcgtt
     1381 ctgatgcgca atcttcagca catgataggg gtgcatcaga tagtgcagct cgccgcgctc
     1441 accgctttca ggcgcttccg tgccctgcca gcgaaagtcg ccatggaggg gcgggggggc
     1501 tgcccagccc atcgcctgca acacctgctc gccatgctgg tgctcgatgc gcgccagctt
     1561 gcggaacagt tcggcgactt ccgcgttgtt gtgcgcctcc atcgcgtcgg cgaattcggc
     1621 atagcgctcg gcggcttccg tttccatggc ataggcatag gtcatcaatt ccgccacgga
     1681 ctcgataccc ggctcgcgcg caccgcgtgc gcgccgcggt ttcttcgcca ccttgcgcac
     1741 agtggcgcgc gttgcagatg gcttgccgga ggcggggcgt ctggcgctca taacgcgaaa
     1801 tcccgttcga gatccgcaac ggtcgcctcg ccgatgccga tcggcggctg aaagggctgg
     1861 cgcgtgccgc tgtagaggat gccgatgtag aagccgtcgt cggtcatgat ccggcgcgcg
     1921 gcgcgcacgg gatcgtcggt cgttgccacc ggcgcgggcc gcacggcctt tttccactcg
     1981 cgctgctcgg ggcggtaggt gacgcacggg ctcagcacct cgacgaagga aaacccgggc
     2041 gtgcggatgg cctgcgcgat gatctcggcg gtgccgcccg ggtcgccgga gaaggcgcgc
     2101 gcgacgaagt tcgcgccggc ggcgagcgcg atcaccagcg gatgaaacgt gcgcaggccg
     2161 gtgccgccgg gagaaagctt cgaatcccag tccggctcgg tggtcgggga gggctgcccc
     2221 ttggtcatgc cgtagacgtg gttgtccatc acgacgtagg tgagatcgac gttgcgccgg
     2281 cacgcgtgca ggaaatggtt gccgccgatc gagtagccgt cgccgtcgcc gccggtcacc
     2341 accacggtga gatcgggacg cgcgagcttg agcccggcgg cggccgcgag cgcccggccg
     2401 tgcacgccgt gaaatccgta gcaggtcgtg taggccggaa tgcgcgagga acagccgatc
     2461 cccgacacca cggcgacgtt ctccggacgc aggccgagca tcgccagcgc ctttgtgagc
     2521 gacgacagca ccgagaagtc gccgcagccg gggcaccaga tcggcttgta gccggacttg
     2581 aaatccttcg ccgcgggcgc ctcgccggtc gcgacaggca caggccgatt catgcgtggt
     2641 cccattcggc gaggacccgg ccgacctcgg acggacgcag cgccagcggg cccggccgcg
     2701 cgaggctctt cacctcgccg ggcaggtcgt attgcgagcg caggtagcga tacagttgcg
     2761 cgccgccgtt ctgctcgacg acgaggacgc gtcgcgcgcc gtcgagcgcc cgggcgaatt
     2821 ccgccgggcg cgccggcgcg agcagtcgga tggaaatcat ccgggccgat acgccgcgcg
     2881 cgcgcgccgc cgcgagcgct tcgcgcaccg gccccgtgct cgatcccagc gtgatcaccg
     2941 cgatttcgcc gtcgccctcg acatcagccc agagcgggcc gtagtttgcc tgggcgagct
     3001 tgcggctgcg tttggcgagc tgcacgtaat ggtccgcggc ctcgctcgag ggcacgccgg
     3061 actcgtcgtg ctccaggccg tccgcggtat aggcgatgcc cgcggtgccc ggaatcgcca
     3121 tcggcgatac gccggatgcg ctgtcggcat agcgctggta attcgccgcg cccgccgccg
     3181 cggtctcgcg cgcgcccgcg aagctcaccg ccggcgaggc atcgatgacc gcgcgcgact
     3241 ggcccatgaa ctggtcggac agcaccagcg ccggcacttg caacgactcg gcgagatgca
     3301 ccgcccactg cgtggtcacg atgcaatcgg cgatcgagtt gggcgcgagc acgatccgcg
     3361 gcgcatcgcc gtgcaagccg ttgaccgcga tagcgatgtc gctttgctcg gtcttcgccg
     3421 gaatgccggt cgagggtccg ccgcgcatca cgtccacgac caccaccgga acctcggccg
     3481 cgaccgctag gccgagcgct tcggtcatga gcgacagccc cggacccgcg gttgcggtga
     3541 gcgccggaac tccgccgtag gacgcgccga tcgccatatt gatcgaagcc agctcgtcct
     3601 ccgcctgcac cagcacgccg cctacctgcg cgagcgcggg cgccatccat tcgagcattt
     3661 ccgtcgccgg cgtgatcgga taccccgcca caaagcgtac gccgccgcgt atcgcgccca
     3721 agcccgcggc ctcgttgccg gtaatgagcc agcgcgggcc cgactgcacg ggtcgcgcca
     3781 gcatgaagga tgcgccgatg tccgatgccg cggcacgccc cgcggccacg gcggcaaggc
     3841 tcgcggccac ggctgcgtgg gcggtgccgt tcagcgactt ttcgacgacg gcgtcgacgg
     3901 ctccggccgg caagccgagc acgccggcgg caatgccgag cgcgatcatg ttcggccagc
     3961 tgccgggaac cgatttcgac aggttcttca tcggcagctc gacgcaacgc gccgcgccct
     4021 tgacatagga ttcaggcggg gcgccttggg cgggatcgac gatgatcagg ctccccgggc
     4081 caagcggaat ttccgacgcc aagcggtgga caccctgcca atcgagcgcg atcagcacgt
     4141 cgaaatggtc atcgaggctc tcgaccggtt gcggcgcgaa gcgcagcagc gccgccgcct
     4201 cgccgccgcg aatctgcggg ccgctgatgc gcaccatcaa tccataccag ccggcgcgcg
     4261 ccgcggcttc caacagcatg ttcccggcgg tcatgacgcc cgcccccccg ctgcccgcga
     4321 tcgccagcga cagactgcgt gccgccttcg acaccgctac agtatgtgcc atatcactgt
     4381 ttttccagga gtttggtgtg ggctatgccg ccaaactgcc gggggttgac tgaattatcg
     4441 tatttcagta ctgtaatact atatttggcc tcgggcatta gtcaagcccg ggctttgaag
     4501 cgaggaggac cgatgccagg cgacgctgac ctgaagaacc acgatctgac ggaaggcgtg
     4561 gtcgtccccg gcgtgcgcgt ggagaggcgc cccgcgaaga cccccgacgg caagcacgcc
     4621 gccggcctct acaacgcctg gatctggatc gacaaccccg cgcagtacaa ctcctacacc
     4681 accgatatcg tcaagggcct gatccacgcg ttccgctcgg cgtccaacgc gcgcgacgtg
     4741 gtatgcacgg tgttcaccgc ggtgggcgac aaggcgttct gcaccggcgg caacaccaag
     4801 gaatacgccg aatactacgc cggccggccg caggagtacc gccagtacat gcggctgttc
     4861 aacgacatgg tgtcgtcgat tctggcctgc gacaagccgg tgatctgccg cgtcaacggc
     4921 atgcgcatcg gcggcgggca ggaactcggc atggcgtgcg acttctcgat cgcgcaggac
     4981 ctcgcgcgct tcggccaggc cggcccgaag cacggctccg cgccggttgg cggggccacc
     5041 gatttcctgc cgccattgat cggcgccgac cgcgccatgg tcgcgtgcgt aatgtgcgag
     5101 ccgttctccg cgcacaaggc ctaccactgg ggcatgctcg ccgacatcgc gccggccctg
     5161 aaagtggacg gccggttcat cgccaatccg ctggtcgaaa cgcagcgctt cgccgacgaa
     5221 tatggccgca tcgttttcgg cgacttcaag accggggaag cggcacggca gggcaaggcg
     5281 ctgatggcgc gcggccaggt cgatttgtcg atgctcgacg ccagggtcga ggacatgtgc
     5341 gccaggctgt tgctgacctt tcccgaatgc acgacgaaga ccatcgagga attgcgcaag
     5401 cccaagctcg aggcgtggaa ccgcaacaag gagaatcacc gtgcatggct cgggcttaat
     5461 atggtgaccg aagggcgcgc cggcttccgc gccttcaatg aaggtcccaa ggacgaccgc
     5521 gaggtcgatt tcatcgcgct gcggcaggca ttggcgcggg gcgagggctg gagcgacgcg
     5581 ttgaccgaac gcatccagcc cggccgcaag aaggccgacg catgaacgat gcgccgctca
     5641 gggtctggct ggaccgcggt ggcgcgctgt tgcggttgcg cctgtcccgc ccgcaagcca
     5701 acatcatcga tggaccgatg gtcgcggcgc tgcgtgccgc ctttggtgag caccgcgcga
     5761 ccgccggact gcgcggtgcg ctgctcgacg ccgaaggccc gcacttcagc ttcggcgcct
     5821 cggtcgaaga gcacctgccg gagcggtgcg ccggcctgct cgccgcgctg cacgacctga
     5881 tcggcgaaat gttcgattgg ccggcgccga tcctcaccgc tgtccagggg cagtgcctgg
     5941 gcggcgggct cgagctcgcc tgcgccggaa gcctgatttt cgccgcggcg gacgccaaac
     6001 tcggccagcc cgaaatcagg cttggcgtgt ttgcgccggc ggcaagctgc ctgctgccgg
     6061 agcgcatcgg acaggccgcc gccgaggaac tgctgctgtc cggccgcagc atcagcgcgc
     6121 aggaagcgct cgcgctgggc ctcgccaccg cgatagccga agacgcgcaa gccgcggcgc
     6181 tcgactggtt cgaccggcac ctcgcggaca agagcgccag ttcgctcgcg cttgccctgg
     6241 ccgcggcgcg cgccgcgcgc ggggccgggc tgcggcgccg ccttcggaaa atcgaggcgc
     6301 tctatctcga ccggctgatg aaaacgcgcg acgccaacga ggggctcagg gccttcctcg
     6361 ccaagcgcgc gccgaaatgg gagcacgcgt gatgaccgac tcagtggcga aaatcgtcga
     6421 gcgctgccag gcgctgtacg aggacctcaa cttcgaggcg gtcaagcgct ggaaggccgc
     6481 cgcgccgggc cgcaaggcga tcggctacat gccgctctac gtgccgcgcg agatcgtgca
     6541 tgccgcgggc atgctgccgg tgggcatcct cggcggcggc gaccagctgg aagtgatcca
     6601 gggcgacgcg tattaccaga gctacatctg ccggatcccg cgctcgacga tcgagctggg
     6661 gctgaccggc aggctcgatt gcctggacgg catgctgttt ccgtcgatct gcgacgtgat
     6721 ccgcaacctg tccggcatgt ggcagatcat gttccggggc aaatacgtgc gctatttcga
     6781 cgtgccgcag aacttcgacg acgcgatcgg cggcgagtat tacgtccacg agctggaggt
     6841 attgcgcgag gacttggctc ggctcgccgg cgccgaggtg ggcgacgctg cgctgcgcag
     6901 ctcgatcgcc ctctacaacg agaaccggcg cgtggtccgc gagctctacg cctaccgcgc
     6961 gcgcgagccc tggcaggcgc cgacttccga ggtgtatctg gtgctgcgcg ccggcatgct
     7021 gctgccggtc gaagagcaca cgcgcctcat ccgcgattac ctcgccgcca ctgcggcgga
     7081 gccgcggccg aaacgcgaca acgcacgcgt cgcgctcacc ggcgcgttct gcgagcagcc
     7141 gccgctcgcg ctcatcaagt cgatcgaaat ggcgggctgc tacatcgtcg atgacgactt
     7201 catgctggtg ctgcgctggc tgctcgacga cgtgccggag agcggcgacc ccctgcagaa
     7261 cctggcgcag tcgttcctgc accgttcggt atccaccgcg tcgaagtacg aagcgcggcg
     7321 cgaggacaag ggccgctacc tcctgcagca ggtgcgcaac accgccgccg agggcgtgat
     7381 cttcgcggcg ccgtcattct gcgatcccgc gctgctcgac cggccgatgc tgctcgacgt
     7441 tctcgcgcag cacggcatcc cctgcaccgc gttcaagtac gcggagaaca ccggccagat
     7501 ggcgccgatt cgcgagcagg cgggcacgtt cgccgattcc atcaaactct ggag
//
LOCUS       DBNGWZ010000633         9757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3129071, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000633 DBNGWZ010000000
VERSION     DBNGWZ010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3129071"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..930)
                     /gene="hda"
                     /locus_tag="AC16CX_19875"
     CDS             complement(220..930)
                     /gene="hda"
                     /locus_tag="AC16CX_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HIR3014803.1"
                     /translation="MKQLALDFASPPLPTLENFIAGRNGELLQNLRRLAARQAHERFL
                     YVWGRPGSGRSHLLRGAVAEMQRAGAAAAYIACAAGTRFKDGLERMDCVALDDVDRLD
                     DDGQVAAFGLYNALREHSGALLAGGNAPPVQLKLREDLVTRLAWGLVYQVHALTDEEK
                     AKALADYASDRGFRLLPEVCDYLLTRASRDLPSLLATLDALDRYSLETKRPVTVPLAR
                     ELLQAAEQRRAGSGIRDA"
     gene            1085..2134
                     /gene="purM"
                     /locus_tag="AC16CX_19880"
     CDS             1085..2134
                     /gene="purM"
                     /locus_tag="AC16CX_19880"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3014804.1"
                     /translation="MKKLDAPQPLTYRDAGVDIDAGDRLVENIKPFAKKTLREGVLAG
                     IGGFGALFEITRKRYREPVLVAGTDGVGTKLKLAFELNRHASIGIDLVAMSVNDILTH
                     GAEPLFFLDYYACGKLDVATATEVIKGIAAGCERAGCALIGGETAEMPGMYPVGEYDL
                     AGFAVGVVEKSRIIDGAGIAEGDAVLGLASSGAHSNGYSLIRKIIAVKGVDLSAKFHG
                     RQLSDAILEPTRIYVKPVLKLARSITVKGLAHITGGGLVDNVPRVLPDRFAARIERAA
                     WTLPPLFLWLKEQGNIADAELYRVFNCGIGMVIVVADNDAAAAVRMLKAAGETAWRIG
                     RIVRRDRNGPQVVLV"
     gene            2289..2951
                     /gene="purN"
                     /locus_tag="AC16CX_19885"
     CDS             2289..2951
                     /gene="purN"
                     /locus_tag="AC16CX_19885"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3014805.1"
                     /translation="MLDKNLVILISGRGSNMEALLDARLPARVAAVISNNPQARGLGV
                     AKTRGLFTAVVDHRAFPDRTSFDSALAQEIDRHRPDLVVLAGFMRILTEPFIERYRGR
                     LMNIHPSLLPAFTGLDTHRRALAAGVRIHGCTVHFVTPAVDSGPIIVQAAVPVLPRDT
                     EDRLAARVLAEEHRIYPQAVRWFCEGRLHLGPNGTVTIDAARGAAGARDGALVSPPLE
                     SR"
     gene            2960..3670
                     /locus_tag="AC16CX_19890"
     CDS             2960..3670
                     /locus_tag="AC16CX_19890"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR3014806.1"
                     /translation="MNMQLHAALAFFAVTGAGASADPVSAPRMVSASYNWLRNGEHVA
                     VMNETFEAHNNNYRIVSESSAVGLLALFRRDRIVFTSSGQITSSGLRPQRFEGRRDDR
                     TEVRAEFEWGNEQLKLIHEGRNDAIALPSGTQDRLSVMYQFMFRQFDQLRQLDFAMTN
                     GRKIERYRYDITPNVDLDTPLGRMSTLHLVKRREPGDNGTEIWIAPQHRYLPVKVLII
                     ERDGTRQEQIITRLELKP"
     gene            3667..4686
                     /locus_tag="AC16CX_19895"
     CDS             3667..4686
                     /locus_tag="AC16CX_19895"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR3014807.1"
                     /translation="MTIRKRFAIAALISLAMHGLIISSTWVPVPQPPGEPRPLVARLA
                     PPPEIKPAVPKPRVRAPRRAAPVPVPAVPVVATASPLVLPESLPDAAPAEAVAEQPAA
                     PEPPQRIALAAESSAAGAGSLPRRGRITYTLLYGNDRSYVGKAVQSWEIEADTYLLSS
                     EAETGGIVELFRPQRLRYLSQGRITPQGLQPESFLMSRTRRGQNEAAQARFNWGAGSL
                     TFGPTRDQKSVSLPASAQDFMSFIYQYALTPPAPGRFRVPITTGTRFEVYEIEVLPEE
                     SIESPIGTLRALPVRQLPRPGEESIQIWLAAEYHYLPVRIRHYDREGNFSGEQVVSEI
                     RIGEE"
     gene            4796..6049
                     /locus_tag="AC16CX_19900"
     CDS             4796..6049
                     /locus_tag="AC16CX_19900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR3014808.1"
                     /translation="MGITRLQLEAATEALKLVLKFDRPADSILHDFFRTRRALGSHDR
                     AFVADTVYGVLRRKRSVDCLARDGTPRHMLLAYLARIAGVSVRELAASLKQDEQAWLA
                     RVKAAPLDGLPLAVRAELPDWVVERLGLHHAEAGILALARGLNEPAPLDLRVNTLRAE
                     RDEVIRALAADGVEAVATPYSPVGVRVKGHPPINKHPLFNNGTLEVQDEGSQLLCYLV
                     APKRHEMVVDFCAGAGGKSLMLGALMRSHGRIYAFDVSAARIARLRPRLKRSGLSNLY
                     PQPIRNENDVRVKRLAGKIDRVLVDAPCSGFGTLRRNPDLKWRQSPQSVEELKLKQAA
                     ILRAAANLVKPGGRLVYATCSLLAEENEDIVTAFLAERADFQQLDCSDILRESRIALD
                     TGPLLRFLPHLHGTDGFFAAVMARP"
     gene            6154..6396
                     /locus_tag="AC16CX_19905"
     CDS             6154..6396
                     /locus_tag="AC16CX_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014809.1"
                     /translation="MALGEWYELRVAGSAGTAAGISAGIHEGIAVGENGRLCSLSSGC
                     ARRFESKQQALDYLGKIKVSGDYQFEAVLCGTRAAA"
     gene            complement(6437..7495)
                     /locus_tag="AC16CX_19910"
     CDS             complement(6437..7495)
                     /locus_tag="AC16CX_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014810.1"
                     /translation="MSGVIRRNHYDVTDRVFVADPRVVCEEVCALLRRLEPGANLRPV
                     ELAFDVFSSLYAGTMTGYVGCETWYHDAQHSLDCALVMARLIEGHEHGATDGRMGERR
                     AQLGVIAALFHDAGYIRKRTDEEQHGAQFTLFHVRRSGDFLAEFLPMVGFGQEAPMAE
                     QLVHFTGYEVALDKIEVRHPLDRRLGFMLGTADVLAQMSDRCYLEKCRDFLYPEFEIC
                     GLAGAPHGDGVKPLYSSREEIVRKTPEFHDKIWKERMDGYFESVYRYAATHFGGHNLY
                     LDVIDGHLRRLREVLQANQIHEGLRRRADCVNAGPLCVILGLQPKGDPGVRAASRPSA
                     YKHRARAGAVDPRDYVPT"
     gene            complement(7492..8715)
                     /locus_tag="AC16CX_19915"
     CDS             complement(7492..8715)
                     /locus_tag="AC16CX_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014811.1"
                     /translation="MNIPQRERGRRPGVQPGGGSTTRSKDRIPATDAKVLARLDDALH
                     GLWRYGRNMMLAWCEADSAISILAVPNYLPAEMLSGGNAGEPAAIERGRAVQELIDGR
                     KQIAPRRMRKLASLLGVRPQRIALDAAARLPRPREVEELLRRYAVSRFDDRAVVLFDI
                     VGFSLSDPLGQLTQLNSLAYSINVAHERLRQRVSNIDIARTTTGDGYYVWNRHSGVEA
                     NIHLYHLMHLVLADNAIARAKSRPELVPALKTAYHIGSHYEFFQAEALRPTRYSYIVG
                     DVTVQIARLVERALPGQVMVGEFNVAMPESEKSETRIRMNTTAFIDRLQESLRVVHGM
                     VLSGERVMSIKCYLTGPRDDAGGYGITRHLIADKHGRDHAAYNAKINIYREQGAPIFL
                     GLQEGELGAQTGPRS"
     gene            complement(8845..>9757)
                     /locus_tag="AC16CX_19920"
     CDS             complement(8845..>9757)
                     /locus_tag="AC16CX_19920"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3014812.1"
                     /translation="ARLTSHEIDKHCALDEQGAALLKQAIARLGLSARGYLFYAPQMN
                     ARVAEQMKFEDDLRNALLQEQYVLHYQPRFDFVSGRIPGLEALIRWMHPERGMVSPGE
                     FIPLLEETGMIIEVGRWALKRAALDYAGWSAAGLKPPRIAVNVSPVQLRRRDFVEYMK
                     DALATVEKTSERIDIEITESMLMEDIEGTIGKLQAMQAMGLHIAVDDFGTGYSSLSYL
                     ARLPINSLKIDRSFVIQMNKGPEQMAIVSTVISLARALNLKVVAEGVETEEQANLLRL
                     LRCDEVQGYLFGRPIPPEEIGRLLPLA"
BASE COUNT         1664 a         3244 c         3167 g         1682 t
ORIGIN      
        1 tcgaagaagc gctggttgcg cgcctcgtac acctcgcggt cgagcacgcc ctgctcgatc
       61 aggaactgcg cccactcgaa atcgctgtcg ccggcgagca gcgtgttatc gagatcgaac
      121 aggaccagtc gattgctcaa tcctcaatcc tcaatcctgg ttcccgcgtc ccgcatcccg
      181 cacccctggg ggaaaaaggg tcctgacccc ttttccgcat cacgcatcgc gtatcccgga
      241 gcccgcgcgc cgctgctccg cggcctggag cagctcgcgc gcgagcggca cggtgaccgg
      301 gcgtttggtc tcgagcgagt agcggtcgag cgcgtccagc gtcgccagca gggaaggcag
      361 atcgcggctc gcccgcgtca gcaggtagtc gcagacctcg ggcagcagcc ggaagccgcg
      421 gtccgacgcg taatcggcga gcgctttcgc cttttcctcg tcggtaagcg cgtgcacctg
      481 gtaaaccagg ccccaagcga ggcgggtcac caggtcctcg cggagcttga gctggactgg
      541 aggcgcgtta ccgcccgcca gcaacgcccc gctatgctcg cgcagggcgt tgtacaggcc
      601 gaacgcggcg acctgcccgt cgtcatcgag ccggtccacg tcgtcgagcg cgacgcaatc
      661 catgcgctcg aggccgtcct tgaaccgggt gccggcggca cacgcgatgt aggcggcggc
      721 cgcccccgcg cgctgcattt ccgccaccgc gcccctgagc aggtggctgc ggccgctgcc
      781 gggccgcccc cagacgtaga gaaagcgctc gtgcgcctgg cgcgcggcga ggcgtctcag
      841 gttctgcagc agctcgccgt tccgccccgc gatgaaattt tcgagcgtgg gcaacggcgg
      901 gctggcgaaa tcgagcgcga gctgtttcat cttgccggga acgacccggt gtcatttacg
      961 gtaaaatccg cctgccttgg acaggttgga ggacgcagcg ggaagcgaag gcggccgacc
     1021 cgcgcatatc ttagcccaaa acccggaact tcccgacacc ggacccgcct ttcgagaaca
     1081 ccgcttgaag aagcttgatg ccccgcaacc gctgacctac cgcgacgccg gcgtggacat
     1141 cgacgccggc gaccggctgg tcgagaacat caagccgttc gcgaaaaaga cgctgcgcga
     1201 gggcgtgctc gccgggatcg gcggtttcgg cgcgctgttc gaaattacgc gcaagcgtta
     1261 ccgcgagccg gtgctggtgg ccggcaccga cggcgtgggc accaagttga agctcgcctt
     1321 cgaactgaac cggcacgcca gcatcggcat cgatctcgtg gcgatgagcg tcaacgacat
     1381 cctcacgcac ggcgcagagc cgctgttctt tctcgactac tacgcgtgcg gcaagctcga
     1441 cgtggcgacc gcgaccgagg tcatcaaggg catcgccgcg ggctgcgagc gcgccggttg
     1501 cgcgctgatc ggaggcgaga ccgccgagat gcccggcatg tatccggtgg gcgaatacga
     1561 cctcgccggc ttcgccgtcg gcgtcgtcga aaaaagccgc atcatcgacg gcgcgggcat
     1621 cgccgaaggc gacgccgtgc tgggactcgc ctccagcggc gcgcactcca acggctactc
     1681 gctgatccgc aagatcatcg ccgtcaaggg cgtggacctg tcggcgaagt tccacggcag
     1741 gcagttgagc gatgcgatac tggagccgac gcgcatctac gtgaaaccgg tgctgaagct
     1801 cgcccggtct atcacggtca agggtctcgc ccacatcacc ggcggcggcc tcgtggacaa
     1861 cgtgccgcgc gtcctgcccg atcgcttcgc ggcgcgcatc gagcgcgcgg cgtggaccct
     1921 gcccccgctg tttctatggc tgaaggagca gggaaacatc gccgacgccg agctctaccg
     1981 cgtattcaac tgcggcatcg gcatggtcat cgtggtcgcg gacaacgacg ccgcggcggc
     2041 ggtgaggatg ctgaaggcgg ccggcgaaac tgcgtggcgc atcggccgca tcgtcaggcg
     2101 cgaccggaac gggccacagg tcgtcttggt ctaacgaccg atgacggaaa ggcgtgatag
     2161 gtgataagtg acaggtgata ggagccagct tccgcgcgag cgcgggtgac ttatcactta
     2221 tcacttatca ctcatcactt atcacgcttc ttggcgcagc ccagctcgtg ggtcattttt
     2281 aacaccggat gcttgacaag aaccttgtaa tcctgatctc cgggcgcggc agcaacatgg
     2341 aggcgctgct ggacgcgcgg ctgccggcgc gggtcgcggc ggtcatcagc aacaacccgc
     2401 aggcgcgcgg cctcggcgtg gcgaagacgc gcggcctctt caccgccgtc gtggaccatc
     2461 gcgcgtttcc cgaccgtacc tcgttcgact ctgcgctcgc gcaggagatc gaccgccacc
     2521 ggcccgatct cgtcgtgctc gcaggtttca tgcgcatcct cacggagccc ttcatcgagc
     2581 gctaccgcgg gcggctgatg aacattcacc cctcgctgct gcccgcgttc accgggctcg
     2641 acacgcatcg gcgggcgctc gcggccggcg ttcgcatcca cggatgcacc gtgcatttcg
     2701 tgaccccggc ggtggacagc gggccgatca tcgtgcaggc cgcggtgccg gtgctgcccc
     2761 gcgacaccga ggatcggctc gccgcgcggg tgctggcgga ggagcaccgc atctacccgc
     2821 aggcggtgcg ctggttctgc gaagggcggt tgcatctggg cccgaatggc accgtgacca
     2881 tcgatgcggc gcgcggcgcg gccggcgcgc gcgacggcgc tctggtttcg ccgccgctcg
     2941 aatcccgttg agtcgcgcga tgaatatgca gcttcacgcg gcgcttgcgt ttttcgccgt
     3001 cacgggcgcg ggcgcgtcgg cggaccccgt atccgcgccg cgcatggtat cggcgagcta
     3061 caactggctg cgcaacggcg agcacgtggc cgtgatgaac gaaacatttg aggcgcacaa
     3121 caacaactac cgcatcgtga gcgaaagcag cgccgtcgga ttgctggcgc tgttccgcag
     3181 ggaccggatc gtcttcacca gcagcgggca aatcacttcg agcgggctcc gtccgcagcg
     3241 cttcgaaggg cggcgcgacg acaggaccga ggtgcgcgcg gaattcgagt ggggcaacga
     3301 gcaactcaag ctgatacacg aaggccggaa cgacgctatc gcgctaccgt ccggcacgca
     3361 ggaccgcctc tcggtgatgt atcaattcat gttccggcag ttcgaccagc tcaggcagct
     3421 cgacttcgcg atgaccaacg gccgcaagat cgagcgctac cgctacgaca tcacgcccaa
     3481 cgtcgatctc gacacgccgc tcgggcgaat gagcacgctg cacctcgtca agcgccgcga
     3541 gcccggcgac aatggcaccg aaatctggat cgcgccgcag caccgctatc tcccggtcaa
     3601 ggtgctgatc atcgagcgcg atggcacgcg ccaggaacag atcatcacca gactggaact
     3661 caagccgtga cgatccggaa acgcttcgcc atcgcagcgc tgatttcgct cgccatgcac
     3721 gggctgatca tctccagcac ctgggtgccg gtcccgcagc cgccgggcga gccgcggccg
     3781 ttggtcgcgc ggctggcgcc gccgccggag atcaagccgg ccgtgcccaa gcccagggtc
     3841 cgtgcgccgc gtcgcgccgc gccggtcccc gtcccggcgg tgccggtggt tgccaccgca
     3901 agtcctcttg ttctgcctga atccctcccg gatgccgcgc ccgccgaagc cgtggcggag
     3961 cagcctgccg ctcccgaacc gccgcagagg atcgcgctcg ccgcggaatc gagcgccgcc
     4021 ggcgcgggca gcctgccgcg caggggacgc atcacctaca cgctgctcta cggcaatgac
     4081 cgctcctacg tcggcaaagc ggtgcagtcg tgggagatcg aagccgacac atacctgctc
     4141 tcgagcgagg cggaaaccgg cggcatcgtc gagctgttcc ggccacagcg cctgcgttac
     4201 ctgagccagg gccggatcac ccctcaggga ttacagcccg aatccttcct gatgagccgg
     4261 acccggcgcg ggcagaacga ggcggcgcag gcgcgcttca actggggcgc gggcagcctc
     4321 accttcggcc cgacacgcga ccagaaaagc gtgtcgctgc ccgccagcgc acaggatttc
     4381 atgagcttca tctaccagta cgccctgacg ccgcccgcac ccggccgctt ccgggtgccg
     4441 atcaccaccg gcacgcgttt cgaggtgtac gaaatcgagg ttttgcccga ggagagcatc
     4501 gagtccccga tcggcacgct gcgggcgctg ccggtgaggc agctgccgcg cccgggcgag
     4561 gagagcatcc agatctggct tgccgccgag taccactacc tgcccgtccg catccggcac
     4621 tacgatcgcg agggcaattt ctccggcgag caagtggtca gcgagatccg catcggcgag
     4681 gagtaaatgc gggtgattga gtaattaggt gactaagtga ttgagtaacc cccggggcca
     4741 cgttgttgat cactcattca cctaatcact cagtcactta gtcactcagt tgcccgtggg
     4801 tattacgcga ctacagctcg aggcggcaac ggaggcgctc aagctggtgc tgaaattcga
     4861 ccgtcctgcc gactcgattc ttcacgattt cttccgtacc cggcgcgcgc ttggatctca
     4921 tgaccgggcc ttcgtcgctg atacggttta cggcgtgctg cgccgcaagc gcagcgtgga
     4981 ctgcctggcg cgggacggca cgccgcggca catgctgctg gcctatcttg cgcgcatcgc
     5041 cggggtgagc gtccgcgagc tcgcggcgtc gctcaagcag gacgagcaag cctggctcgc
     5101 gcgcgtcaag gccgccccgc tcgacggctt gccgctcgcg gtgcgcgccg agctgccgga
     5161 ctgggtggtg gaacggctcg ggctccatca cgccgaggcc gggatacttg cgctcgcgcg
     5221 cgggttgaac gagccggcgc cgctcgacct gcgcgtcaac acgctgcgcg ccgaacgcga
     5281 tgaagttata cgggcgctcg ccgccgacgg cgtcgaggct gtcgcaacgc cctattctcc
     5341 cgtcggagtg cgcgtgaagg gacacccgcc gatcaacaag catccgctct tcaataacgg
     5401 tacgctcgag gtacaggatg aaggcagcca attgttgtgt tatctcgtgg cgccgaagag
     5461 gcacgagatg gtggtggatt tctgcgccgg cgccggcggc aagagcctga tgctgggcgc
     5521 gctcatgcgc tcccacggcc gcatctacgc cttcgacgtc tcggcggcgc gcatcgcccg
     5581 gctcaggccg cggctcaagc gctcgggact gtccaatctc tatccccagc cgatccgcaa
     5641 cgaaaacgac gtccgggtga agcgccttgc cggaaaaatc gaccgggtgc tggtggacgc
     5701 gccgtgcagc gggttcggca cgctgcgccg caatcccgat ctcaaatgga ggcagtcgcc
     5761 gcagtcagtg gaggaattga agctcaagca ggcggcaatc ctgcgcgcgg cggcgaatct
     5821 cgtgaaaccc ggcgggcggc tcgtgtacgc cacctgcagc ctgctcgccg aggaaaacga
     5881 ggacatagtc actgcgtttc tggcggaacg cgcggatttt caacagcttg attgctcgga
     5941 tatcctgcgc gaatcccgca tcgccctcga taccgggccg ttgctgcgct tcttgccgca
     6001 tttgcacggc accgacggtt tcttcgccgc cgtcatggcc cgcccctagg tggcggttcg
     6061 aaaaaaatct tttctgcgag caggcccatc ctcgccagac ccgtggtaga gtgttcagcc
     6121 aatgtaagca tggtcacact caggggagag gccgtggcat tgggtgagtg gtacgaattg
     6181 cgggtcgccg gcagcgccgg cactgccgcc ggcatctctg caggaatcca cgagggtatc
     6241 gccgtcggcg aaaacggccg tctctgttcg ctgagttcgg gctgcgcgag gcggttcgaa
     6301 tcgaagcagc aggcgctcga ttacctcggc aagatcaagg tgtccggcga ctaccagttc
     6361 gaagcggtcc tctgcggcac gcgggccgcc gcctgatcat ggcagcgttc aaatcaggcg
     6421 actccctcgc cgaagctcac gtcggcacgt aatcgcgcgg atccacggcg cctgctcgcg
     6481 cccgatgctt gtaggccgat ggccgcgatg ccgcgcggac gccggggtcg cccttcggtt
     6541 gcaggccgag gatgacgcac agcggcccgg cgttgacgca gtcggcccgc cgccggagcc
     6601 cttcatggat ctggttggcc tgcagcactt cgcgcagccg cctcagatgg ccgtcgatca
     6661 cgtccagata gaggttgtgg ccgccaaagt gggtcgccgc gtagcgatag acgctttcaa
     6721 agtagccgtc catgcgctcc ttccagatct tgtcgtggaa ctcgggcgtc ttgcgcacga
     6781 tttcctcgcg cgagctgtag agaggcttca cgccgtcgcc gtggggcgcg cccgcaaggc
     6841 cgcaaatctc gaattcggga tagaggaagt cgcggcattt ctccaggtag caacggtcgg
     6901 acatctgcgc cagcacgtcg gcggtgccga gcatgaagcc caggcgccgg tccagcggat
     6961 gacgcacctc gatcttgtcc agcgcgacct cgtagccggt gaagtgcacg agctgttccg
     7021 ccatcggggc ttcctgcccg aagcccacca tcggcaggaa ttcggccagg aagtcgccgc
     7081 tgcggcgaac gtggaacaag gtgaactgcg cgccgtgctg ctcctcgtcg gtgcgcttgc
     7141 gaatgtagcc ggcgtcgtga aacagcgccg cgatcacacc cagctgggcg cgacgctcgc
     7201 ccatgcgccc gtccgtagcc ccgtgttcgt gcccttctat cagccgcgcc atgaccagcg
     7261 cacagtcgag gctgtgctgc gcgtcgtgat accaggtttc gcagcccacg taaccggtca
     7321 tcgtccccgc gtagaggctg ctgaacacgt cgaacgccag ttcgaccggc cgcaggttgg
     7381 cgcccggttc caatcgtcgc aacaaggcgc agacctcctc gcagacgacg cgcggatcgg
     7441 ccacgaatac gcggtccgtg acgtcgtaat gattgcgccg gatgaccccg ctcacgatcg
     7501 cggacctgtt tgcgcgccga gttcgccttc ttgcagtccg aggaaaatcg gcgcgccctg
     7561 ctcgcggtag atattgatct tggcgttgta ggccgcatga tcgcggccgt gtttgtcggc
     7621 gatcaggtgg cgcgtgatgc cgtagccgcc cgcgtcatcc cgcggtcccg tcaggtagca
     7681 cttgatggac atcacccgtt cgccggacag caccataccg tgtacgacgc gtaagctttc
     7741 ctggaggcga tcgatgaacg cggtggtgtt catccggatc cgcgtttccg atttctccga
     7801 ctccggcatc gcgacgttga actcaccgac catgacctgg cccggaagcg cgcgctccac
     7861 gaggcgcgcg atctggacgg tcacgtcacc gacgatgtag ctgtagcgcg tgggccgcaa
     7921 agcctcggcc tgaaagaact cgtagtggct gccaatgtgg tacgcggttt tgagcgccgg
     7981 aacgagctct ggccgggatt tcgcccgcgc gatggcgttg tcggcgagca cgaggtgcat
     8041 caaatggtag agatggatat tcgcctcgac cccgctatgg cggttccaga cgtagtagcc
     8101 gtcgcccgtc gtcgtgcgcg cgatgtcgat attcgacacg cgctgccgca ggcgttcgtg
     8161 cgccacgttg atcgagtagg cgaggctgtt gagctgcgtc aactggccga gcggatcgct
     8221 cagcgagaaa cccacaatgt cgaacaggac gacggcgcgg tcgtcgaatc gggaaacggc
     8281 gtagcgccgt aggagctcct ccacctccct aggccgcggt aggcgggcgg cggcgtcgag
     8341 cgcgatccgt tgcggtctga cgccgagcag cgaggcgagc ttgcgcatgc gccgtggcgc
     8401 aatttgcttg cggccatcga tgagttcctg caccgcccgg ccgcgctcga tcgcggccgg
     8461 ctcgcccgcg ttgcccccgg agagcatttc cgccgggagg tagttgggta ccgcgagaat
     8521 gctgatcgcg gagtccgcct cgcaccaggc aagcatcatg ttgcgtccgt aacgccagag
     8581 cccgtgcagc gcgtcgtcga gacgcgcgag gactttggca tcggtagcag gaatcctgtc
     8641 cttcgatcgc gtcgtcgagc cgccgccggg ctgcacaccg gggcggcgac cccgctcgcg
     8701 ttgcgggatg ttcacgccat cgttcctgcg tcgtaccgtg gatttacggg gtcggttcat
     8761 gaaaggcggc gctgatcctc ggcggctttg ctgccagtgc tgctgcttga tttttgcagt
     8821 atgggattac gacaagctgc agcctcaagc cagaggcagc aatcgtccta tctcttcagg
     8881 tggtatcggg cggccgaaca ggtagccctg cacttcatcg cagcgcagca ggcgcagcag
     8941 gttggcctgc tcttccgttt ccaccccttc ggccaccacc ttgagattga gcgctcgcgc
     9001 gagggagatc accgtggaaa caatcgccat ttgctcaggg cccttgttca tctggatgac
     9061 gaatgagcga tctatcttca gcgagttgat cggcagccgc gcaagatagc tcaacgagga
     9121 ataaccggtg ccgaagtcgt cgacggcgat gtgcaggccc atggcctgca tcgcttgcag
     9181 cttgccaatg gtgccctcga tgtcctccat gagcatgctc tcggtgatct cgatgtcgat
     9241 ccgctcggac gttttttcca ccgtagccag cgcgtccttc atgtattcga cgaaatcccg
     9301 gcgccgcagc tgcaccgggg acacgttgac cgcgatgcga gggggtttca gaccggcagc
     9361 gctccatccg gcgtagtcga gcgccgcccg cttgagcgcc cagcgtccca cctcgatgat
     9421 catcccggtt tcctccagta acgggatgaa ctcgcccggc gacaccattc cgcgctcggg
     9481 gtgcatccag cggataagcg cctcgagccc ggggatccgc ccgctgacga aatcaaagcg
     9541 cggctggtag tggaggacgt attgctcctg cagcaaggcg ttgcgcaggt catcctcgaa
     9601 cttcatctgc tcggcgacgc gcgcattcat ctgcggcgcg tagaacaggt acccgcgcgc
     9661 tgaaagccct agtcttgcaa tcgcctgttt cagcagcgcc gcgccctgct cgtccagtgc
     9721 gcagtgctta tcgatctcgt gcgatgtgag ccgcgca
//
LOCUS       DBNGWZ010000634         1814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2624087, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000634 DBNGWZ010000000
VERSION     DBNGWZ010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2624087"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1007
                     /locus_tag="AC16CX_19925"
     CDS             69..1007
                     /locus_tag="AC16CX_19925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3014813.1"
                     /translation="MKLKWPTDRLDYVPITDRPPLPLPGGARVVVWPCVNVEHWMIDA
                     PMPRTVVNPPGGVAKSVPDIPNWAWHEYGNRVAFWRLKKIFDEFGVAASLVLNGSVCD
                     VYPQIVDACLASGWDLVGHGWIQRALPAVPDQRKMIHRVFKRLKEYSGRPPKGWLGPA
                     LAETHDTVDWLAEAGFEYVADWVLDDQPVDLRTRHGTLVSLPYTQELNDLSMLLIQGH
                     RAAEYADRAIDQFDQLIEDAAGGSSRVMCFTMHPYIMGVPHRAKHLRRILEHVCKRSD
                     ALVWTGEQILDWYKSVRPKTGWPPVKSRGKKRVARP"
     gene            1024..>1814
                     /locus_tag="AC16CX_19930"
     CDS             1024..>1814
                     /locus_tag="AC16CX_19930"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3014814.1"
                     /translation="MNSQRSATHAGEAVAEALREEGVERVYSVPGSHIHPIYDGLSRV
                     SSIRMVTCKQEPNVSLMADAYGRLTGKPGVCVVTAGPGCLNSMAGVAQAYGAASPVVH
                     ISGAVPLKADREAFHGVDDPDFVHEMFKHITKWSARIERLSDIPEVMAKAFHIARSGR
                     PGPVHVELPRLSDYSEYILQEEAVALPAYKLIPTVVPKPDPEDVDHFARRLMESRSPV
                     LAAGKGIIRKGAMQELAQLSMKLQAPVVFAQDAIGVIPEAHPFFAG"
BASE COUNT          327 a          593 c          594 g          300 t
ORIGIN      
        1 gggcaaacga gaaaatcaaa tcgagctatc tcgggatctg actccgttcg aatgaggcga
       61 ggacagggat gaagctcaag tggccgaccg accgcctcga ttacgttccg atcaccgacc
      121 ggccgcctct gccgttgccg ggcggcgcca gggtggtggt gtggccgtgc gtcaatgtcg
      181 agcactggat gatcgatgcg ccgatgccgc gcacggtggt caatccacct ggaggcgtgg
      241 cgaagagcgt gcccgacatt ccgaactggg cgtggcatga atacggtaac cgcgtcgcgt
      301 tctggcgctt gaagaagatc ttcgacgagt tcggcgtcgc cgcctcgctg gttctcaacg
      361 gctcggtatg cgacgtctat ccgcagatag tcgatgcatg cctcgcgtca ggctgggatc
      421 tggtcggcca cggctggatc cagcgcgcgc tgccggcagt gcccgaccag cgcaagatga
      481 tccatcgcgt attcaagcgc ctgaaggaat attccggcag gccgcccaag ggctggctcg
      541 gcccggctct tgccgagacg cacgataccg tggactggct ggccgaggcc ggcttcgagt
      601 acgtcgccga ctgggtgctc gacgaccagc cggtcgactt gcgcacccgg cacggcacgc
      661 tggtcagcct gccctatacg caggagctga acgatctgtc gatgctgctg atccagggcc
      721 accgcgccgc cgagtacgcc gaccgcgcaa tcgaccagtt cgaccagctg atcgaggatg
      781 ccgccggcgg ctcttcgcgc gtcatgtgct tcaccatgca cccgtacatc atgggcgtgc
      841 cgcaccgggc gaagcatctg cgccgcatcc tcgagcacgt gtgcaagcga tccgacgcgc
      901 tggtgtggac cggcgagcag atcctcgact ggtacaagag cgtgcggccg aaaaccggct
      961 ggccgccggt caaatcgcgg gggaaaaagc gggttgcccg gccgtgacgc aacccgtcga
     1021 cccatgaatt ctcagcgttc ggcgacgcac gcgggcgagg cggtcgccga ggccctccgg
     1081 gaggaggggg tcgaaagggt ctacagcgtc cccgggtccc acatccaccc gatctacgac
     1141 gggttgagcc gggtgagctc catccggatg gttacctgca agcaggagcc gaacgtctcc
     1201 ctcatggccg acgcctacgg ccgcctcacc ggcaagcccg gagtatgcgt cgtcaccgcc
     1261 ggacccggct gcctcaactc gatggccggc gtcgcccagg catacggggc agcctcgcca
     1321 gttgtgcaca tcagcggcgc cgtgccgctg aaagccgacc gtgaagcctt tcatggtgtg
     1381 gatgaccccg attttgtcca cgagatgttc aagcacatca ccaagtggag cgcccgcatc
     1441 gagcgcctct ccgacattcc ggaggtaatg gcgaaggcct tccacatcgc gcgcagcggg
     1501 cggccggggc ccgtgcacgt cgagctgccg cgcttgtccg actacagcga gtacatcctg
     1561 caagaggaag ccgtggccct cccggcttat aagctcatcc ccaccgtagt ccccaagccc
     1621 gatcccgaag acgtggacca cttcgccagg cggctcatgg agtcgaggtc tccggtgctt
     1681 gcagcgggaa aggggatcat ccgcaagggc gcaatgcagg agctcgcgca actctcgatg
     1741 aagctgcagg cgccggtggt gtttgcgcag gatgcgatcg gcgtcatccc ggaggcacac
     1801 cctttcttcg cggg
//
LOCUS       DBNGWZ010000635         2790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2624088, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000635 DBNGWZ010000000
VERSION     DBNGWZ010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2624088"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC16CX_19935"
     CDS             complement(<1..1176)
                     /locus_tag="AC16CX_19935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014815.1"
                     /translation="MPSRLEVLIVGAGPAGIAAACAASESGLRAGVVDDNPAPGGQIW
                     RSQIGDRKGADWLERFRSAGVELLTETRVIDAPARGVLHAESAEASLELGYDKLILAT
                     GARERFLPFPGWTLPNIMGAGGLQALVKGGLPIEGKRVVVAGTGPLLLAAAAYLRKRG
                     AEIPLIAEQARWNRLARFGLALGPAKLAQAAELGWDLRGVPYRTGCWPLSAEGDGKME
                     SVVLQCGKKSWREPCNYLAYGFGLAPNLELPALLGCAFRNGCVEVNEWQETSVTGVYC
                     AGEPAGIGGVDLALSEGQIAGYAAAGRPDRARAAFRARRKSLRFRDALHRAFALREEL
                     KTLVTPETIVCRCEDVSFGRIQEHRSWKSAKLQTRCGMGPCQGRVCGPAVEFLLGWTA
                     "
     gene            complement(1169..1417)
                     /locus_tag="AC16CX_19940"
     CDS             complement(1169..1417)
                     /locus_tag="AC16CX_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3014816.1"
                     /translation="MPEPIPLLVNGKVISVAAGCTVAAAVLLAGETAFRRSVRGEPRG
                     PLCGMGICFECRVTIDGVAHSRSCQILCRPGMEVRTDA"
     gene            complement(1410..2534)
                     /locus_tag="AC16CX_19945"
     CDS             complement(1410..2534)
                     /locus_tag="AC16CX_19945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3014817.1"
                     /translation="MSETADVVVIGAGIVGAACAEALSREGTRVAVVESGVIGGGATA
                     AGMGHLVVLDDSEAEFALTRYSRDLWHELASSLPLEVEFETCGTLWVAADEEELAAVH
                     RKAAFYAARGVRAEVLDPAALAAAEPNLRPGLAGGLLIPDDAVIYAPCAARWLIEQAH
                     TRGASVRVGGPAIEVLPDGVLLGDGTRISAGLTVCATGSWAVELFPNLGVRPRKGHLA
                     ITDRYPGFVHHQLVELGYLKSAHGSATESVAFNVQPRATGQVLIGSSRQFGATHSTVD
                     APMLRRMLQRAFEYMPGLDRLSVIRTWTGFRAATEDKLPLIGPCPGRDGAYLATGHEG
                     LGITTSLGTARLVADLIAGRKPAIAPEPYLPSRLRQLTHA"
     gene            complement(2531..>2790)
                     /locus_tag="AC16CX_19950"
     CDS             complement(2531..>2790)
                     /locus_tag="AC16CX_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HIR3014818.1"
                     /translation="CPGKAYDRSPCGTGTSAKLACLAADGKLRQGETWRQESIVGSLF
                     EGSFRWSGDCILPSIRGSAYVNSEATLILDPRDPFSTGIRR"
BASE COUNT          478 a         1008 c          902 g          402 t
ORIGIN      
        1 cgccgtccac ccgagcagaa actcgacggc gggaccgcag acgcggcctt ggcaggggcc
       61 catgccgcag cgggtctgaa gcttggcgga tttccaggag cggtgctcct gaatccggcc
      121 gaaggacacg tcctcgcacc ggcaaacgat ggtctcgggg gtgaccaggg ttttcagctc
      181 ctcgcggagg gcgaaggcgc ggtgcagcgc atcgcggaac cgcagggatt tacggcgcgc
      241 gcggaatgcc gcccgcgccc gatcgggccg gcccgccgca gcgtagcccg ctatttgtcc
      301 ctcgctgagc gccagatcca caccgccgat gccggccggc tcaccagcgc agtaaacgcc
      361 cgtgacggaa gtctcctgcc actcgttcac ctcgacacaa ccattccgga aggcacatcc
      421 cagcagcgcg gggagttcca ggttcggcgc caggccgaaa ccgtaggcca ggtagttaca
      481 gggctcgcgc cacgacttct taccgcactg cagaacaacg ctctccatct ttccgtcacc
      541 ctctgcggac aggggccagc atccggtgcg gtacggcacg ccccgcagat cccatcctag
      601 ttctgccgcc tgagcgagtt tcgccggccc gagggcgagt ccgaaacgtg cgaggcggtt
      661 ccagcgggct tgctcggcga tcagcggaat ctcggcgccg cgcttccgca ggtacgccgc
      721 tgccgccagc agcaacggcc ccgtaccggc caccacgacg cgtttcccct cgatcggcag
      781 gccgcccttg acgagcgcct gcaggccgcc cgcgcccatg atgttgggga gggtccagcc
      841 ggggaaggga aggaagcgct cccgcgctcc agtggccaag atcagtttgt cgtagcctag
      901 ctcgagcgac gcctcggcgg attcggcgtg aagcacgccc cgcgcaggcg cgtcgatcac
      961 ccgtgtttcc gtgagcagct cgacgccggc gctgcggaag cgctccagcc agtccgctcc
     1021 tttacggtcg ccgatctgac tccgccagat ctgacctccg ggggccggat tatcgtccac
     1081 cactcccgcg cggaggccgc tctcggaagc cgcgcacgct gcggcgatcc cggccggccc
     1141 ggcgcctaca atcagtacct cgaggcggct aggcatccgt gcggacctcc atccccggcc
     1201 ggcacaggat ctggcagctc cggctgtgcg ccacgccgtc gatcgtgacc cggcactcga
     1261 agcagattcc catgccgcac agcggccctc gtggctcgcc gcgcaccgac cggcgaaacg
     1321 ccgtctcgcc ggcaagcaga acggctgccg ctacggtgca gcccgcggca acactgatga
     1381 cctttccgtt caccagcagg gggatcggct caggcatggg tgagttgccg cagccgggac
     1441 ggcaggtagg gctcaggagc gatggcgggc ttgcgtcccg caatcagatc ggccacgagc
     1501 cgcgccgtcc cgagcgaagt ggtgatgccg agtccttcgt gtccggtggc aagatacgcg
     1561 ccgtcgcggc ccgggcaggg gccgatcaag ggcagcttgt cttcggtcgc cgcgcggaag
     1621 cccgtccacg tacgaatcac cgacagccgg tcgaggccgg gcatgtattc gaaggcgcgc
     1681 tgcaacatgc ggcgcaacat cggagcatcc accgtggagt gcgtcgcacc gaactgccgc
     1741 gaggagccga tcagcacctg cccggtcgcc ctgggctgca cgttgaacgc gaccgactcg
     1801 gtggccgacc cgtgcgcgct cttcaaatac ccgagctcga ccagttggtg atggacgaat
     1861 cccgggtacc gatccgtaat ggccagatgg cccttgcggg gccgaacgcc caagttggga
     1921 aagagctcga cggcccagga tcccgtcgcg cacaccgtca ggcccgccga gatccgcgtc
     1981 ccgtcgccca acagaactcc gtccggcagg acctcgatgg ccggccctcc cacccgtacg
     2041 gaagcgccac gagtgtgggc ctgctcgatg agccaccgcg ctgcgcaagg cgcatagatg
     2101 acggcgtcgt cgggaatgag cagccctccg gcaagtcccg gccggagatt tggttcggca
     2161 gcggcgagcg ccgccggatc gagcacctcc gcgcggacgc cccgagccgc gtagaaggct
     2221 gcctttcgat gaacggcggc cagctcttct tcgtccgccg ccacccacag agtcccgcac
     2281 gtctcaaact ccacctccag cggcagcgac gaggccaact cgtgccacaa atcgcgcgag
     2341 taacgtgtga gggcaaactc cgcctccgag tcgtccagca ccaccaggtg tcccatgccc
     2401 gcggcggtcg ccccgccgcc aatgacgccc gattcgacga cagcaacgcg cgttccttcg
     2461 cgcgacagcg cctccgcgca ggcggccccc acaataccgg cgccgatgac taccacatcg
     2521 gccgtctcac tcatcggcgg attcccgtcg agaacgggtc gcgcggatcc aggatcaggg
     2581 tcgcttccga attcacgtag gccgagcccc tgatggaagg aaggatgcag tcgccggacc
     2641 agcgaaaaga gccttcgaag aggctgccga cgatgctctc ctgccgccag gtctcgccct
     2701 ggcgaagttt cccgtccgcg gccaggcagg cgagcttggc gctggttccg gtgccgcacg
     2761 gagagcggtc gtaggcctta ccaggacaca
//
LOCUS       DBNGWZ010000636         4091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1618400, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000636 DBNGWZ010000000
VERSION     DBNGWZ010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1618400"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1192)
                     /locus_tag="AC16CX_19955"
     CDS             complement(230..1192)
                     /locus_tag="AC16CX_19955"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014819.1"
                     /translation="MRTVCCVVLLFCFIASVASAQQYPSRPIRMIVPQAPGSASDTVA
                     RIIAAELTNQMKQQFVVDNRPGGALMIGLELTSKANPDGYTIGYAMVGALAISPNVVQ
                     KPPLNVLKDLQPIAQTTIGQMLLAAGPSTPFKSVRDVVGYAKQNPGKLFNASSGNGTP
                     GHVGFELFKLMTGTQIVHVPYKGGAAGISDLIAGQVQLMLESTNSITPFAKAGRVRGL
                     AVSGPKRSEALPDIPTIAEAGVPGYEATTWTGIVAPAGMPKPIVARLNAELNKIVASR
                     SFREKVAAIGSEPAGGTPEQFAEFIRKEHAKWADVVGRSGAKID"
     gene            1292..2269
                     /locus_tag="AC16CX_19960"
     CDS             1292..2269
                     /locus_tag="AC16CX_19960"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014820.1"
                     /translation="MLRSIRIRVTVAGIFVLSAAAALPAAGQGVTGRPVRVLVPVPPG
                     GPSDTAIRLILPRLSEGLKQTLVVDNRPGSNGVAGTDIASKANPDGYTLAVGNSGTHA
                     INASLYRKLPYDPVRDFTPIIQMVTSGMVLVANPKVAANSLMDFINAAKKQPGKLNVG
                     IAGATGELSGDALWAQVQLKMNNVRYKGSAPTELAILAGEVDVAMLTPLATQQHIRSG
                     KLKAYGITSAQRSPVLPQVPTIGEQGVQGYDFQIWHGIFAPRSTPDRVIRTVNKEAVQ
                     ALHAPEVKERFSTLGFVIIANTPQEFAAVVKSEVAKFRKVIKESGIELL"
     gene            2332..3411
                     /locus_tag="AC16CX_19965"
     CDS             2332..3411
                     /locus_tag="AC16CX_19965"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR3014821.1"
                     /translation="MRISRWKFWPLTLVVLAGCAAVPEQKGGEDISGPYEVVPNWPKP
                     MYLDWTWGSTAAVWAESPDRVFVFQRGELPVLKQQPPVSGVPPRIATQAKPRWQNCLL
                     VFNRDGEVVEAWRQHDDKFVRPHRIAISPYDPEKHVWLVDDGAHQVFKFTNDGSKLLL
                     VIGEKGKPGNGQYSFNRPTDIAFLPNGDFYVTDGYVNTRVVKYDRNGKYLLEWGKPGK
                     GPGEFNLVHGISIDVDLQRVYVSDRSNSRIQVFDLEGRYVEEWNNIYSPFYLALTRNR
                     RLAVSDGMTQKIMTYDLTGRLLTSWGTFGGLPGMLWGVHQLSVDSEGNLYVAEVFNGR
                     VQKFRPRPGANLSTLIAQTMHSLSR"
     gene            3444..3833
                     /locus_tag="AC16CX_19970"
     CDS             3444..3833
                     /locus_tag="AC16CX_19970"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR3014822.1"
                     /translation="MTTLADASWLDTAGRLLIAGFFLATGVCNLTPAAIRDHVERMAA
                     FGVPLPAATFWFGITLQFTGIALLVTGWHADIGVICLIVFTIAGNAIFHRYWIVADPG
                     RRAVTRILLLDGVAILGGLLLLLQSVR"
BASE COUNT          755 a         1308 c         1308 g          720 t
ORIGIN      
        1 attcccgttt gacgagctgc cggccgccaa ggcctacgtc gagaccaacg cgcaactcgg
       61 caaggtcgtc gtcacgatgg gataaagcgt gacatggtac tgcgtgacat gatacggcgt
      121 gacatgacag tgcgcggcta gccagcaggt accaaacacc agtcacgctt catcatgtca
      181 cgcttcacca tgtcacgatt catcacgtca cgcttcacca tgtcacgtct caatcgatct
      241 tcgctccgga gcgcccgacc acgtcggccc atttcgcgtg ctccttgcgg atgaactccg
      301 cgaactgctc gggcgttccg cccgccggct cggagccgat cgccgcgact ttttccctga
      361 acgaacgtga cgcgacgatt ttgttgagct cggcgttgag ccgcgcgacg atgggcttcg
      421 gcataccggc cggcgccacg atgcccgtcc acgtggtcgc ttcgtacccc ggcacgcccg
      481 cttccgcgat cgtgggaatg tccggcagcg cctccgagcg cttgggcccg ctgaccgcga
      541 gcccgcgcac gcggcccgcc tttgcgaacg gcgtgatcga attcgtgctc tccagcatca
      601 gctgcacctg gcccgcgatg aggtccgaga ttcccgccgc cccgcctttg tacggcacgt
      661 gcacgatctg cgtgccggtc atcagcttga acagctcgaa gcccacgtgt cccggcgtgc
      721 cgttgccgga ggacgcattg aaaagctttc caggattctg cttcgcgtaa ccgacgacgt
      781 cgcgcacgct tttgaagggc gtggacggcc cggccgccag cagcatctgg ccgatcgtcg
      841 tctgcgcaat cggctgcagg tccttcaata cattgagcgg tggcttttgc accacgttgg
      901 ggctgatcgc gagcgcgccg accatggcgt aaccgatggt atagccgtcg gggttggcct
      961 tggatgtcag ctccaaccct atcatcaacg ccccgccggg ccggttgtcc accacgaact
     1021 gctgcttcat ctggttcgtc aattccgcgg caatgatgcg ggcgaccgtg tccgacgcgc
     1081 tgccgggcgc ctgcggcacg atcatccgga tcggccgcga cggatactgc tgggctgacg
     1141 cgacgctcgc gatgaagcag aacaacagga cgacacagca tacagtgcgc atggccttct
     1201 cctccctatg ccgcgcgagc gtagcatgag tcacgccgtc gggcacgact agtggtaggc
     1261 taatgcgcct gccacttggg aggaggcccc tatgctccgt tccatccgga tacgcgtcac
     1321 ggttgcggga attttcgtgc tctccgcggc ggctgcgctg cccgctgccg ggcagggcgt
     1381 cacgggacgg ccggtgcgcg tgctggtgcc ggtgccgccg ggcgggccct ccgacaccgc
     1441 gatccgcctc atcctgccga ggctctccga ggggctcaag cagacgctgg tggtggacaa
     1501 ccgccccggc tcgaacggcg tcgcgggcac cgacatcgcg tcgaaagcca accccgacgg
     1561 ctacacgctg gcagtgggca acagcggcac ccatgccatc aacgcgagcc tctatcgcaa
     1621 gctgccctac gatccggtgc gcgatttcac gcccatcatc cagatggtga cgagcggcat
     1681 ggtgctggtc gcgaacccga aggtggccgc gaactcgctc atggacttca tcaacgctgc
     1741 gaagaagcag cccggcaagc tcaacgtcgg cattgcgggg gcgaccggcg agctctccgg
     1801 cgacgcgctg tgggcgcagg tgcagctcaa gatgaacaac gtgcgctaca agggcagcgc
     1861 gcccaccgag ctcgcgatac tggcgggcga agtggacgtg gcgatgctga cgccgctcgc
     1921 cacccagcag cacatccggt cgggcaagct caaggcatac ggcatcacga gcgcccagcg
     1981 ctcgcccgtc ctgccgcagg tgccgaccat cggggagcag ggcgtgcagg gctatgactt
     2041 ccagatctgg cacggcatct tcgcgcccag gagcacgccg gaccgggtga tccgcaccgt
     2101 gaacaaagaa gccgtgcagg cgcttcacgc tccggaggtc aaggagcgct tcagcacgct
     2161 gggcttcgtc atcatcgcca acacgccgca ggagttcgcc gccgtggtca agagcgaggt
     2221 cgcgaagttc cgtaaggtca tcaaggagtc cgggattgaa ttactgtgac gcagtgacgt
     2281 cacgtcatca cctcgtcagt tcgtcatgca tttgggagaa aaggagaaga catgcgcatc
     2341 agtcgttgga aattctggcc gctgacgctg gtcgtcctgg caggatgcgc ggcggttccg
     2401 gagcaaaaag ggggggaaga catctcgggc ccgtacgagg tggtcccgaa ctggccgaag
     2461 ccgatgtacc tggactggac ctggggctcg acggccgcgg tctgggccga gtcgccggac
     2521 cgggtattcg ttttccagcg cggagagctg ccggtgttga agcagcagcc acccgtatca
     2581 ggcgttccgc cgcggatcgc gactcaggcc aagccgcgct ggcagaactg cctgctggta
     2641 ttcaaccgcg acggcgaggt ggtggaagcg tggcggcagc acgacgacaa gttcgtgcgg
     2701 ccgcaccgca tcgcgatcag cccctacgat ccggaaaaac atgtctggct ggtggacgac
     2761 ggcgcgcacc aggtgttcaa gttcaccaac gacggctcaa agctcttgct ggtgatcggc
     2821 gagaagggca agcccggcaa cggtcaatac agcttcaacc gcccgaccga catcgcgttc
     2881 cttccgaacg gcgacttcta cgtcaccgac ggctatgtca atacgcgcgt ggtgaagtac
     2941 gaccggaacg gcaaatacct tctcgaatgg ggcaaacccg gcaagggtcc cggtgaattc
     3001 aacctggtgc acggaatttc catcgacgtg gacctgcagc gggtctatgt ctcggaccgc
     3061 tcgaattcgc gcatccaggt attcgatctg gaaggccgat acgtcgagga atggaacaac
     3121 atctattcgc cgttctacct cgcgcttaca cgcaaccggc ggctcgccgt gtccgatggc
     3181 atgacgcaga agatcatgac ctacgatttg accggcaggc tgctcacgtc gtggggtacg
     3241 ttcgggggat tgccggggat gctgtggggg gttcaccagc tgagcgtgga cagcgagggc
     3301 aatctctacg tcgccgaggt tttcaacggg cgcgtccaga aattccggcc gagacccggt
     3361 gccaacctct caacgctgat cgcgcagacg atgcacagcc tgtcgcgctg acgcggtggt
     3421 gggagatgag tgataggtga tgagtgacga cgctcgctga cgcttcctgg ctcgacaccg
     3481 ccggccggct gctgatcgcc ggctttttcc tggcgactgg cgtatgcaac ctgacgcccg
     3541 cggcgatccg cgaccacgtc gagcgcatgg cggcgttcgg cgtgccgctg cccgccgcca
     3601 cgttctggtt cggcatcacg ctgcaattca ccggcatcgc gctgctcgtc actggctggc
     3661 acgccgatat cggcgtcatc tgcctgatcg tcttcacgat cgccggcaac gcgatttttc
     3721 accgctactg gatcgttgcc gaccctggcc gccgtgccgt gacccggata ttgctgctcg
     3781 acggcgtggc cattctggga gggctcctgc tgctgcttca gagcgtacgg taatgggcgc
     3841 gccgtgagcc gagggcttgc gcgtcgcgcc catgaagtaa aatttcaggc tgcttcgcag
     3901 cgaaggaggg gaagccatgc caatacgtaa gccggcggcg cgcatgaaga tggtgccgaa
     3961 aaacaagctg aagcagaacg tcaccctgcg cacgatcgag gagatggcgc agcgctgcaa
     4021 gaactgggga aaatgggggc ccgacgacga gctcggcacg ctcaactacg tgacgaggaa
     4081 ggacatcgtc a
//
LOCUS       DBNGWZ010000637        17520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2123857, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000637 DBNGWZ010000000
VERSION     DBNGWZ010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 17520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2123857"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="AC16CX_19975"
     CDS             <1..609
                     /locus_tag="AC16CX_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3014823.1"
                     /translation="TAQYLEYVEMMRARSLELAAEYLLMAAMLIEIKSRMLLPRPAAG
                     EVEEDPRAELARRLLEYERMKLAARRLDELPQAGRDFSLVQVLFEQTVAERLPEVNPE
                     DLRMAWLSLLTRAAANRHHRITREQLSVRDYMTRILRRLQETRYVEFQELFDPAQGIA
                     ALVVTFLAVLELARESLIEVSQQAAYAPIYVKLRSGHLAAVE"
     gene            623..1252
                     /gene="scpB"
                     /locus_tag="AC16CX_19980"
     CDS             623..1252
                     /gene="scpB"
                     /locus_tag="AC16CX_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3014824.1"
                     /translation="METNSPDLGQVKTVLETVLLVSQEPLPLPELKKLFDEELSSDTL
                     RRVLDDLRRDWKDRGVELVHVASGWRFRAKPEYQRYVDRLNPQKPPRYSRAVLETLAI
                     ITYRQPVTRGDIEEIRGVVVSPNIIRTLEARGWIEVVGHKEVPGRPELLATTRTFLDD
                     LNVSSLDELPPLDDLGSLVERAGVQPQPPLVEEPEPEAAAVKEPQQAAA"
     gene            complement(1308..2450)
                     /locus_tag="AC16CX_19985"
     CDS             complement(1308..2450)
                     /locus_tag="AC16CX_19985"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3014825.1"
                     /translation="MNRLFVPSVFLAAAAISVLAFFPLPGLGQTFKTQEHSFRVVRLV
                     EGLEHPWGLAFMPDGRMLVTERPGRLRMVGKDHKLDPRAVSGLPQIADYGQGGLLDVA
                     LHPRFAENYLVYLSYTARGQGGVGTEVARGRLAGDRLENVQVIFRQQPKGSSGRHFGS
                     RLVFDREGYLYITLGDRGEQERAQKPDDHAGSVIRLHDDGRVPTDNPFVGKAGWKPEK
                     LTLGNRNMQGAALHPATGLLWTHEHGPQGGDEINVIRAGVNYGWPVITYGVNYFTGTQ
                     IGEGTRKPGMAQPLHYWMPSIAPSGMAFYTGDKFPGWRGDLFVGALRDEMLVRLKLDG
                     EKVLKEERMLKNVLGRIRDVRDGPDGYLYLLTDEPNGVLARLEPAG"
     gene            2653..3666
                     /locus_tag="AC16CX_19990"
     CDS             2653..3666
                     /locus_tag="AC16CX_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR3014826.1"
                     /translation="MKYCGIDLHGNNSWVVILDEKDRVLYSRRHANDLSRLLPVLATH
                     RSELCGIAVESTFNWYWLVDGLQEAGYRVLLANTAAIKQYDGLKHRGDESDARQLAHL
                     LRLGLLPEGYIMPKAERAVRDLARKRMQLVQQRTAQILTIESSLSRHSGWKLSSNRIK
                     CLWQADLDEMRLGEMPMLGLKANVAVMQTLQTQIDSLEKVLADYCRAEPGYQLLRTVI
                     GIGEVLATVILLETGSIERFADAGNYASYCRCVGSAHLSNGKKKGQGNTKNGNRYLAW
                     AFVEAANFAVRSCEAARRFYQRKKATRNNTVAIKAVAHKLARACYHMLKTQTPFCVDR
                     CFA"
     gene            complement(4107..4655)
                     /locus_tag="AC16CX_19995"
     CDS             complement(4107..4655)
                     /locus_tag="AC16CX_19995"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3014827.1"
                     /translation="MPARVQCPGRWNLKICIVSDSHDHREPLAAAVAEAKALGAQAVL
                     HCGDLVAPSTLHAIIGLGLPIHLIHGNNTGDIFHLHRFAQEPGNRLHYYGQDGSLALA
                     QRRIFLVHYPHYARAMALTGDYDLVCNGHEHRAVVERVRNVKGEETLRIDPGTVAGVS
                     APATYVFGDLQTLEFGIRPVPL"
     gene            4741..5529
                     /locus_tag="AC16CX_20000"
     CDS             4741..5529
                     /locus_tag="AC16CX_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014828.1"
                     /translation="MMFTSNPFAGISASIPPAVMQTYVVAMIILVAAGTLFDIVHKGS
                     AKYFFDHWRKSKSQGGKQIGGGELVSLAIKTGLVDVLASGEFCNARRRVAHLLTMYGF
                     LIYFITSAILVFGYPTPATPTPAILPLLWWMGGLMVCLGGYWFWFFIRVDVAAEGNSP
                     LRIMRADLFILSLLGSATLGLIWAFLQTRGGSGANVLFGLYLIATTVLFGSVPWSKFA
                     HMFFKPAAAFEKRASYANGTRSNLPAPADKPEVFGSAREQPRHY"
     gene            5572..6036
                     /gene="aprB"
                     /locus_tag="AC16CX_20005"
     CDS             5572..6036
                     /gene="aprB"
                     /locus_tag="AC16CX_20005"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HIR3014829.1"
                     /translation="MPTFVYMTRCDGCGHCVDICPSDIMHIDKTYRRAYNIEPNMCWE
                     CYSCVKACPQHAIDVRGYADFAPLGHSVRVLREEAKATISWRLKFRNGHEKNFVSPIR
                     TTPWGSIKGPAEYDAPRPEDFAKQELAHEPEGLNIKGGLPALRPDQLKQGVV"
     gene            6036..7880
                     /gene="aprA"
                     /locus_tag="AC16CX_20010"
     CDS             6036..7880
                     /gene="aprA"
                     /locus_tag="AC16CX_20010"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019418 - sulfide oxidation [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HIR3014830.1"
                     /translation="MGLFGGRKTVFVDADILVIGGGMAGCGATYESRYWGRDLKIVCV
                     EKANIERSGAVAQGLYAINCYMGMQWGENQPEDHVRYARNDLMGLVREDLGYDIARHV
                     DASVHKFEEWGLPMMRDPKTGRYQREGKWQIMIHGESYKPIVAEAARKAATEVYNRIM
                     VTHLLMDSAKANSVGGAVGFNVRTGDYYVFRAKAVIIAAGGASHIFKPRAVGEGMGRT
                     WYAPWSSASAYGLPIQIGAKMTQMENRIVLTRFKDGYGPVGAYFLHLKTYTQNGYGEE
                     YESKWYEHTKALVGNYIDRHPVPTCLRNHAFLEEMKAGRGPIRMVTKEAFQDPHKEVV
                     GWENFLGMTIGQAVVWASQNIDPKHINPELTTSEPYVMGSHATCSGAWASGPEDCAPA
                     EYQWGYNRMMTVAGLFGAGDTIGGSAHKFSSGSFTEGRIAGKAAVRYVNDMGKAQPQV
                     SEQEYRDLEKAIFQPLENYRVGRNEIVAGTVSPSYLLPIHGLQRLQKIMDEYVGGISA
                     HYMTNDVLLNRGLELLDMLKEDMSRMGAEDLHQLQRVWELHHRLLAAESVTHHTLFRQ
                     ETRWPGYYYRGDFMKLDDQNWHCFTLSRYDRNTGKFEMEKAPVYHIID"
     gene            complement(7964..9181)
                     /gene="sat"
                     /locus_tag="AC16CX_20015"
     CDS             complement(7964..9181)
                     /gene="sat"
                     /locus_tag="AC16CX_20015"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003166.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HIR3014831.1"
                     /translation="MVGLVNPHGKGKLKPLLLDGAALADERKRAQSLPRVAVSSREKG
                     DLIMLGIGGFTPLAGFMTRADWEGVCDGYRMANGVFWPIPITLSTDEVIADAIKTGGE
                     VALADPDGGGILAVLKVTEKYRIDKAHECMSVFKTTDLEHPGVRMVMQQGDVNLAGPV
                     RVLSDGGFRARYRELFLTPAETRAKFESLGWSRVAAFQTRNPMHRSHEYLAKVAIESC
                     DGVLVHSLLGNLKPGDIPAEVRTRAISVLIEKYFVPKTVVQAGYPLDMRYAGPREALL
                     HAVFRQNYGCAYQILGRDHAGVGNYYGPFDAHRIFDEIPKDALEIQPMKIDWTFWCYA
                     CGGMASARTCPHKDGDRLLVSGTKLRKWLSEGSPVPPEFSRPEVLEILREYYAGLDAH
                     EKVEVRLSGHSAR"
     gene            9487..10902
                     /locus_tag="AC16CX_20020"
     CDS             9487..10902
                     /locus_tag="AC16CX_20020"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR3014832.1"
                     /translation="MLLVGLAGLSFTATSAPAAETSDPFDTDRYVPTAPGVRWQSPFE
                     LPRVPEAPSAALPVTDRPLTLPELTEFALQHNPKSRQAWFAARAAAAAIGIERSDDFP
                     QISALISGQRNESGGQTGNQNPWLTRYGPSVTLSYLLFDFGVGDSRVRAAEYQALAAS
                     LAQNRVLQDVVFQVEQAYYRLLGTEQLVRANELFLKSVSTSLEATQRRREGGLATVAD
                     VFRAETQVAQGQLNLTRSLGELEKARGALASAVGLPVNATVKVMTIESPPRIQEITTS
                     LTEILNRAKANRPDLVAAEAQARSARALADATARSTLPSISVNATTGRTFYNNRAYTD
                     TNSILLNLSIPIFTGFNYTYSVRQAEARAEQAEASRDVLYRQTEMDVWQSYYDVQTAV
                     GGVSTTEVQLRAAQQAAEATLARYQAGFGSLLDLITAQVEESNARVLRIQSYLDWFTA
                     VARLNYSIGASDKSTYVVQTR"
     gene            10899..12122
                     /locus_tag="AC16CX_20025"
     CDS             10899..12122
                     /locus_tag="AC16CX_20025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR3014833.1"
                     /translation="MKRLWMAVMFIAIAAGSYGAWWWWQQSGASAAEGRSAKAKGKGK
                     GAPLFVKTTRAVAKPMPVLIEAVGTVESEHSVQIRAQVSGVLEKVLFKEGDKVKAGQL
                     LFQIDPRTFEAAYRQSQAQLARDMAQLENAKVQQDRLEPLLKREFITRQEFDVAVTST
                     KSLEATVAADQALIEQARIQLEFSRIHAPITGRTGTLAIKPGNLVPTAGGGVPLVTIN
                     STDPILVSFSIPERQLEEIRRYQNDKDMRIEILPDRNGPSVADGKLVFIDNTVTPQTG
                     TVLLKTRVQNKEELLWPGQFVNVRTVLKIELEAVVVPEVAVQPGQQGNFVYMVEDGRA
                     KIQPVRVSRQIGGEVVIAEGIKTGDIVLTEIPQAIAPGAQVQISDAEGAGKGRDGKGK
                     GRGGKGRSDKGEAKS"
     gene            12146..15238
                     /locus_tag="AC16CX_20030"
     CDS             12146..15238
                     /locus_tag="AC16CX_20030"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3014834.1"
                     /translation="MNTNLSRIFIERPVATSVLVMAVVFFGMIAFRSLPVNDLPNVDF
                     PTIQVTANLRGANPEVMASTVATPLERQFSRIAGLDTMSSNSTNGVTRVTLQFSLDRS
                     IDSAAQDVQTAITQAMRFLPADADPPYLRKVNPADAPILFIAVTAQTLPLTKLDEFAD
                     NHIAQRFSMVSGVAQVSVFGSAKYAVRVYVNPESLAKRGLGLDKVVSAIQNANSNLPS
                     GVMSGAARNYTVKSSGKLDRAASFSDLIVAYSNGMPVRLSDIGRAEDSVENVKNASWV
                     NGERAIYFAVFRQPGSNTVEVVRNIRALFPEIEREAPPGVKMHVLNDRSVFISASIHD
                     VNFTLVLSMILVVLVILVFLRNATSTLITALVLPTSVAGTFAAMYLVGFSLNNLSLMA
                     IILAVGFVVDDAIVVLENITRHMEMGKDRMRAALDGSKEIAFTVLSMTISLAAVFLPI
                     LFMGGMLGRLFREFVVTVGAAVLISGAISLSMTPMLCSLMLRPTHEHGRVYRWLERIF
                     DIARDAYGASLCWTMRHRGLMLAGSGIVLVLIFVVYKMVPQGFIPRQDTGLFFGNIRA
                     PEGVTFSELVRRQKLVADIIQSNPNVEAVLSTAGQGVGGVVGDNVGRVVVRLKPRNER
                     KLSADEVIQELRRAFAGSPQGLRVFLNNPPAINIGGLISTADYQLVIQATELETLYQP
                     AQELEGKLRQLGVVTDVSSNLELRNPEIQVSILRDRAAALGVNPQQLETTLYNAYGGR
                     QISTLYGATDQYYVILELDPRFQRNIDALRSLYIQSNSGQMVPMHAVADIKSGVGPVS
                     VTHYGQLPSVILSFNLALGASIGQAVDSVQEVTRVLPSGITSLFVGSAKAFEDAFRTL
                     PILLLITIIVIYMILAILYEHYGHPITILTALPFAGFGALLMLLLFNMELNIFSFVGI
                     ILLVGLVKKNGIMMVDFALQLQRDKGLSAQDAIVEACLIRFRPIMMTTMSAIFATLPI
                     ALGYGAGAETRRPLGVAVVGGLLFSQFLTLYVTPTFYVSMERLIGYFRRHRAVEAVGR
                     "
     gene            15380..15456
                     /locus_tag="AC16CX_20035"
     tRNA            15380..15456
                     /locus_tag="AC16CX_20035"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15414..15416,aa:Met,seq:cat)
     gene            15678..17212
                     /locus_tag="AC16CX_20040"
     rRNA            15678..17212
                     /locus_tag="AC16CX_20040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            17359..17435
                     /locus_tag="AC16CX_20045"
     tRNA            17359..17435
                     /locus_tag="AC16CX_20045"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17393..17395,aa:Ile,seq:gat)
     gene            17445..17519
                     /locus_tag="AC16CX_20050"
     tRNA            17445..17519
                     /locus_tag="AC16CX_20050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17477..17479,aa:Ala,seq:tgc)
BASE COUNT         3474 a         5175 c         5567 g         3304 t
ORIGIN      
        1 accgcccagt atctcgagta cgtcgaaatg atgcgcgccc gctcgctcga gctcgcggcg
       61 gagtacctgc tgatggcggc gatgctgatc gagatcaagt cgcggatgct gctgccgcgt
      121 cctgctgcgg gtgaggttga ggaggacccg cgcgcggagc tggcgcggcg cctgctggaa
      181 tacgagagaa tgaagctcgc agcgcggcgc ctggacgagc tgccgcaggc cgggcgcgat
      241 ttttcgctgg tgcaggtgct gttcgagcag accgtcgccg agcggctgcc ggaagtgaat
      301 cccgaagacc tgcgcatggc ctggctctcg ctgctcacgc gcgcggcggc gaaccgccat
      361 caccgcatca cgcgcgagca gctctcggtg cgcgactaca tgacccgcat cctgcgccgc
      421 ctccaggaaa cacgctacgt cgagttccag gagctgttcg acccggcgca gggcatcgcc
      481 gcgctggtgg tgaccttcct cgccgtgctc gagctggcac gcgagagcct gatcgaagtc
      541 agccagcagg cggcctacgc cccgatctac gtgaaactga ggagcggcca tctcgcggcc
      601 gtggaatgac ggaaacccca tgatggaaac gaactctccc gacctgggac aggtgaaaac
      661 ggtgctggaa accgtgctgt tggtgagcca ggagccgctc ccgctcccgg agctgaagaa
      721 gctcttcgac gaggagctat ccagcgatac gctgcgccgc gtgctcgacg acctgcgccg
      781 ggactggaag gacagaggtg tcgagctggt gcacgtcgcc agcgggtggc gcttccgcgc
      841 caagcccgag taccagcgct acgttgaccg cctcaacccc cagaagccgc cacgctactc
      901 gcgtgcggtg ctggaaacgc ttgcgatcat cacctaccgg cagccggtca cgcgcggcga
      961 catcgaggag atccgcggag tggtggtttc ccccaacatc atcaggacgc ttgaagcgcg
     1021 cgggtggatc gaggtggtgg gacacaagga agtgcccggc cgccccgaac tgctggcgac
     1081 caccaggaca ttcctggatg atctgaacgt gagctcgctt gacgagctgc ccccgctgga
     1141 tgacttggga tcgctcgttg aacgggcagg cgtgcagccg cagccgccgc tcgtggaaga
     1201 gccggagccg gaagcggcgg cggtcaagga gccgcaacaa gcggccgcct gaaccccgcg
     1261 ccgacagaca acggaccccg aagtatcttg ccgaaccctt tggatcatta tcccgcgggc
     1321 tcgagacgcg ccagcacgcc gttcggctca tcggtgagga ggtacaggta accgtccggt
     1381 ccgtcgcgga cgtcgcggat gcgtcccagg acgttcttga gcatccgctc ctcttttagg
     1441 accttttcgc cgtcgagctt gagacggacc agcatctcat cgcgtaacgc gccgacgaag
     1501 agatcgccgc gccagcccgg aaatttgtcc ccggtgtaga acgccatccc ggaaggcgcg
     1561 atcgagggca tccagtagtg gagcggctgc gccatgccgg gcttgcgcgt gccctcgccg
     1621 atctgggtgc cggtgaagta attgacgccg taggtgatga ccggccagcc gtagttgacg
     1681 ccggcccgga tcacgttgat ttcgtctccg ccctgcgggc cgtgctcatg cgtccacaac
     1741 aagccggtcg cggggtgcag cgccgcgccc tgcatgttgc gattgccgag cgtgagcttc
     1801 tccggcttcc agccggcctt gcccacgaag gggttgtccg tgggaacgcg cccgtcgtcg
     1861 tgcaggcgga tcaccgatcc cgcgtggtcg tcgggctttt gcgcgcgctc ctgctctccc
     1921 cggtcgccga gcgtgatgta gagatagccc tcccggtcaa acaccagtct cgagccgaaa
     1981 tgccggccgc tgctgccctt gggctgctgg cggaagatca cctgaacgtt ctcaaggcgg
     2041 tcaccggcga gcctgccgcg ggcgacctcg gtgccgacgc cgccttgtcc gcgcgcggtg
     2101 taggaaaggt agacgagata gttctcggca aaacggggat gcaatgcgac gtcgagcagc
     2161 ccgccttggc cgtagtctgc gatctgaggc agcccggaaa cggcccgggg atcgagcttg
     2221 tgatccttgc cgaccatccg cagccttccg gggcgttccg tgaccagcat cctgccgtcg
     2281 ggcatgaaag cgagacccca cggatgctcc agcccctcga cgagcctcac cacacggaac
     2341 gagtgctcct gggtcttgaa cgtctgcccc aggccaggga gggggaagaa agcgagaact
     2401 gatatagccg ctgccgccag gaatacggac ggtacgaaga gcctattcac agagcgattc
     2461 atacaaccgg tcttgggcgt cggcgagctc gctggctgtt gcacttgttg tttgaggcca
     2521 agttgtttct cgctttttcc tctgcggttc aatacatggc gtggtctgat aatcatctag
     2581 ccgccctccc tccggcgcga gccggagtga ataaagtaaa ggtgcgaacc gactttatcc
     2641 ttggagggcg acatgaaata ttgtggcata gacctgcacg gcaataacag ttgggtagtg
     2701 attcttgatg agaaggatcg cgtgctctac tcgagacgac atgcgaacga tctatcacgg
     2761 ttactcccgg tgctggcgac acatcggtct gagctgtgcg ggattgctgt ggaatcgact
     2821 ttcaattggt attggctggt cgatgggctg caggaagcgg gttaccgggt gctgttagcg
     2881 aacaccgcgg cgatcaagca gtatgacggg ctgaagcacc ggggggatga atcagatgct
     2941 cgccagctcg cgcacctgct gcgcttgggg ctgctgccag agggatacat catgcccaag
     3001 gcagagcgtg cggttcgcga ccttgcgaga aagcgtatgc aattggtgca gcagcgcacg
     3061 gcccagattc tgaccattga gagcagcctg tcgcgccaca gcgggtggaa gctgagcagc
     3121 aatcgcatca aatgcctgtg gcaggccgat ctggacgaga tgcggctggg tgagatgccg
     3181 atgctcgggc tcaaggccaa tgtcgccgtg atgcagacgc tgcaaacgca aatcgacagc
     3241 ctggagaagg tgcttgccga ctactgccgc gccgagcccg gctaccagct gctgaggacc
     3301 gtaatcggca tcggcgaggt gctggccacg gttatcctgt tggagaccgg cagcatcgag
     3361 cgctttgcgg acgccggcaa ctatgcgtcg tactgccgct gcgtgggcag cgcgcacctg
     3421 tccaacggca agaagaaggg ccagggcaac accaagaacg gcaaccgcta tctggcgtgg
     3481 gccttcgtcg aggcggcgaa cttcgcggtg cgttcctgcg aagccgcgcg gcggttctac
     3541 cagaggaaga aagctacgcg caacaacacg gtggccatca aggcggtggc gcacaagttg
     3601 gcgcgggctt gttatcacat gctcaagacg cagacaccgt tctgcgtcga ccgatgcttc
     3661 gcgtagcgta ggctacggcc ggcgagcccg ttacgggtct gggcatgtaa ccacctgatc
     3721 tgattggacg ccgtgtcgta tgccaacctc agatacctgc aagagatgaa tcgcctggat
     3781 tgcgagccac atgggtctgg agccgcctgg cggcagccac tgatctgccc gcacgacaat
     3841 tggacgcaag acagcaccga gatgcttctg gggcagcgtt gctgccaggg cggagcaagc
     3901 agtcacgctc ccgcttgatc ctgatgggtg actggcgcga ttcaagccac cacacgacga
     3961 agcaatggat gcgataccgg attgcaaggc cctgatgttg gcagcgtcaa cctggtgcga
     4021 taccgctgtc tcgggtcatc gcagggactt cattcgcgct ctttacgcgc ggcggctaat
     4081 gggcgacccg aatcagcgac cgcgcttcaa aggggaaccg gccggatccc gaattccagg
     4141 gtctgaagat cgccgaacac gtaggtcgca ggcgcgctca cgcccgcgac cgtcccggga
     4201 tcgattcgca gcgtctcttc ccctttgacg ttccggacgc gctcgacaac ggcgcggtgc
     4261 tcatggccgt tgcataccag gtcgtagtcg cccgtgagcg ccatggccct ggcgtaatgg
     4321 gggtaatgca ccaggaatat ccgcctttgc gccagggcca gggatccgtc ctggccgtag
     4381 taatgcaggc ggttcccggg ctcctgtgcg aacctgtgca ggtgaaatat atcgcccgtg
     4441 ttgttgccgt gaatcaggtg gatcggcagc cccaatccga tgatcgcgtg cagtgtagaa
     4501 ggcgccacca ggtcgccgca gtgcagtacc gcctgagcgc cgagtgcttt cgcctccgcg
     4561 acggccgcgg cgagcggctc gcgatggtcg tgactgtcgg aaacgatgca gattttcaga
     4621 ttccacctcc ccgggcattg tacccgagcc ggcactaagt attttctatg gcggtataac
     4681 ctttttttcg cgtgcgccac tccctgcgcg tttagaatca gcgcagtaca gaagggggtc
     4741 atgatgttca ccagcaatcc gtttgccggg atttcggcgt ccataccacc cgccgtcatg
     4801 cagacctatg tcgtcgccat gatcatcctg gttgccgcag ggacactttt tgacatcgta
     4861 cataagggga gcgccaagta ttttttcgat cactggcgga agtcgaaaag ccaggggggg
     4921 aagcagatcg gtggcggcga gttggtgtcg cttgccatca aaaccggcct tgtagacgtc
     4981 ctggcatccg gggaattctg caacgcgcgt cgccgggtcg cccatctgct gaccatgtac
     5041 gggttcctga tctacttcat caccagcgcc atcctggtgt tcggctaccc gacgcccgcg
     5101 actccgacgc ccgccattct gccgcttctt tggtggatgg gcggcttgat ggtttgcctt
     5161 ggcggatact ggttctggtt ctttattcgg gtcgatgtcg ccgccgaggg caattcgccg
     5221 ctccgcatta tgcgcgcgga tctgttcatc ctctcgctac tgggcagcgc aacactgggc
     5281 ttgatctggg ccttcctgca aacgagaggc ggttccggcg ccaacgtact cttcggcttg
     5341 tacctcattg ccacgacggt gctgttcgga tcggtgccgt ggtccaagtt cgctcacatg
     5401 tttttcaagc ccgcagcggc cttcgaaaaa agggcgtcct acgcgaacgg cactaggagc
     5461 aacctgcccg cgcccgccga caaacccgag gtattcggca gcgctcgcga acagccccgg
     5521 cactactgat tagcccatca tcgatttcaa atacgggaga tagcgaaagc catgccaacg
     5581 ttcgtttaca tgacccggtg cgacggttgc gggcattgtg tcgatatctg cccgtccgac
     5641 atcatgcaca tcgataaaac ctatcgccgt gcctacaaca tcgagccgaa catgtgctgg
     5701 gagtgctact cctgcgtgaa ggcctgtccg caacacgcca tcgatgtccg cggttatgcc
     5761 gacttcgccc ctctgggcca cagtgtccgg gtgcttcggg aagaggcgaa ggcgacgatt
     5821 tcgtggcggc tcaagtttcg gaacggccac gagaagaact tcgtgtcccc gatccggacg
     5881 acgccctggg gatcgatcaa ggggccggcc gagtacgatg cgcctcgtcc cgaggatttc
     5941 gccaagcagg aactcgcaca cgaaccggaa gggctcaata tcaaaggggg attaccagcg
     6001 ttgcgcccgg atcaactcaa gcagggagtc gtctgatggg actgttcgga ggcaggaaaa
     6061 cggttttcgt tgacgcggac atcctcgtca ttggcggcgg catggctggc tgcggggcaa
     6121 cctacgagtc ccggtactgg ggccgcgacc tgaagatcgt ctgcgtcgag aaagccaaca
     6181 tcgagcgcag cggcgccgtc gcgcagggtc tttacgccat caactgctac atgggcatgc
     6241 aatggggcga gaaccagccc gaggaccatg tccgctacgc ccgcaacgac ctcatgggcc
     6301 tggtgcgcga ggacctgggc tacgacattg cccggcatgt ggacgcctcg gtgcacaagt
     6361 tcgaggaatg gggccttccg atgatgcggg accccaagac cggacgctac cagcgcgaag
     6421 gcaagtggca gatcatgatc cacggcgaga gctacaagcc gatcgtcgcg gaagccgcgc
     6481 gtaaggccgc aacggaggtc tacaaccgga tcatggtcac gcatctgctg atggacagcg
     6541 ccaaggccaa cagcgtcggc ggcgccgttg ggttcaacgt ccgcacgggc gactactacg
     6601 tgttccgcgc caaggccgtg atcatcgccg cgggtggtgc gtcccacatc ttcaagccgc
     6661 gcgccgtggg cgagggcatg gggcggacct ggtatgcgcc ctggagcagc gcgtccgcgt
     6721 acgggctccc gatccaaatc ggcgccaaga tgacgcagat ggagaaccgc atcgtcctga
     6781 cccgcttcaa ggacggctat ggccccgtcg gcgcctactt cctccacctg aagacgtaca
     6841 cgcagaacgg ctatggcgag gagtacgagt ccaagtggta cgagcacacc aaggctctgg
     6901 tgggcaacta catcgaccgg cacccggtgc cgacctgctt gaggaaccac gccttcctcg
     6961 aggaaatgaa ggcgggccgg ggcccgattc gtatggtaac caaggaggca ttccaggatc
     7021 cccacaagga ggtggtcggc tgggagaact tcctgggcat gacgatcggg caggcggtcg
     7081 tctgggcgtc gcagaacatc gaccccaagc acatcaatcc cgagctgacc acgtccgagc
     7141 cttatgtcat gggctctcac gccacctgct ccggcgcgtg ggcgagcgga ccggaagatt
     7201 gtgctcccgc ggaatatcag tggggataca accgaatgat gacggtcgcc gggcttttcg
     7261 gcgccggcga caccatcggc ggcagcgccc acaagttctc gtcggggtcc ttcacggaag
     7321 gccggatcgc cggcaaggca gcggtcaggt acgtgaacga catggggaaa gcccagcctc
     7381 aggtcagcga gcaggagtat cgggacctcg aaaaggcgat cttccagcca ctcgagaact
     7441 accgggtcgg ccgcaacgag attgtcgccg gaaccgtatc gccgagctac ctgctgccga
     7501 tccacggtct gcagcgcctc cagaagatca tggacgagta cgtcggtggc atcagcgccc
     7561 actacatgac gaacgacgtg ctgctcaacc gcgggctcga gttgctggac atgctgaagg
     7621 aagacatgag ccgcatgggt gccgaagacc ttcaccagct ccagcgtgtg tgggagctac
     7681 accaccggct cctcgccgcg gagtcggtga cccatcacac gctgttccgc caggaaacgc
     7741 gatggccggg gtattactac cggggcgatt tcatgaagct ggacgatcag aactggcatt
     7801 gcttcacctt gtcccgctac gaccggaaca ccggcaagtt tgaaatggag aaggcgccgg
     7861 tctatcacat catcgactga catcgtggac caggagctcg caagaaaagc cgccctcaca
     7921 ggcggctttt tcatgggacg ggtcgtccgg aacaggagtg ctgtcaccgc gcggaatggc
     7981 cggacagcct gacctccact ttttcgtgcg cgtccagccc ggcgtagtac tcccgcagta
     8041 tctcgagtac ctctggccgg gagaactccg gcggcaccgg actgccttcc gataaccact
     8101 tgcgcagctt ggtgccggat accagcagcc ggtccccgtc cttgtgcggg caggtgcgcg
     8161 ccgaagccat gccgccgcag gcatagcacc agaacgtcca gtcgatcttc atcggctgga
     8221 tctcgagcgc gtccttgggg atctcgtcga agatcctgtg cgcgtcgaac ggcccatagt
     8281 agttgccgac gcccgcatgg tcgcggccca ggatctgata tgcgcagccg tagttctggc
     8341 ggaacaccgc gtgcagcagc gcctcgcgcg ggccggcata gcgcatgtcg agcggatagc
     8401 ccgcctgcac caccgtcttc ggcacgaaat acttctcgat gaggacggag atcgcccggg
     8461 tgcgcacctc ggccggaatg tccccgggtt tgagattacc gagcagtgaa tgcaccagca
     8521 cgccgtcgca gctctcgatc gccaccttgg cgaggtactc atgcgagcgg tgcatcgggt
     8581 tgcgcgtctg aaacgccgcc accctcgacc agccgagcga ctcgaacttg gcacgcgttt
     8641 cggccggagt caggaaaagc tcgcggtaac gggccctgaa tccgccgtcg gagagcaccc
     8701 tcaccggccc ggcaaggttt acgtcaccct gctgcatcac catcctgacg cccggatgct
     8761 cgagatcggt ggtcttgaac acggacatgc attcgtgtgc cttgtcgatg cggtacttct
     8821 cggtgacctt gagcacggcg agaatgccac cgccgtccgg gtccgcgagc gcgacctcgc
     8881 cgccggtctt gattgcgtcg gcgatcacct cgtcggtcga gagcgtgatc ggaatcggcc
     8941 agaacactcc gtttgccatg cgatagccgt cgcacacgcc ttcccagtcg gcgcgcgtca
     9001 tgaagcccgc cagcggcgtg aatccgccga tgccgagcat gatgagatcg cctttctcgc
     9061 gggagctgac ggcgacgcgg ggcagggatt gcgcccgctt cctttcgtcg gcgagcgcgg
     9121 cgccgtccag caacagcggt tttagctttc cctttccgtg aggattgaca agcccaacca
     9181 tgacaggtgc cggagacaaa tcagggtttc tgtttggtgg caagaggtta gcacgcaaac
     9241 ggtggctgaa aaatggatta tttttattac cggataaggt ccttcgattc gcgcctcgac
     9301 caggccggaa ttggagccta actcgcgaga cgggaaagag aaaatccatg tgatatgatt
     9361 tcccaattat cgattcccgc ttgaggtgcg cgccggtgta agaaccgctc ggaggacccg
     9421 gcagcagcgg taatttacgg gttcttggag gggtgtattc tggtcctgta tacggcacgt
     9481 cggaggatgc tcctggtggg attagcgggg ttgtcgttta ccgcgacgtc cgcgccggcc
     9541 gcagagacca gtgatccctt cgatacggac cgttacgtgc cgactgctcc cggcgttcga
     9601 tggcaatccc ccttcgaact gccgcgggtg cccgaagcgc caagcgccgc gctgccggtg
     9661 actgatcggc cgctgacgct cccggagttg accgagttcg cgctgcaaca caatccgaaa
     9721 agccgccagg catggttcgc cgcccgtgct gcagccgcgg cgataggtat tgagcggtcc
     9781 gacgactttc cccagatctc agcgctgatc agcggtcaac gcaacgaatc gggtggtcag
     9841 accggtaatc agaacccatg gctgacgcgc tacgggccat cggtgacctt gagctacctc
     9901 ctgtttgact tcggtgtggg cgacagccgt gtcagggcgg cggaatacca ggcgttggcg
     9961 gcgtcgctgg cccagaatcg tgtcctgcag gatgtggtat tccaggtgga gcaggcctac
    10021 taccggctgc tgggcaccga gcaactcgta cgggccaatg aattgttcct gaaaagcgtg
    10081 agtacctcgt tggaggcgac ccagcgccgg cgagagggtg ggctggcgac ggtggccgat
    10141 gtgtttcgcg ccgaaaccca ggtggcgcag ggacaactca atctgactcg cagcctggga
    10201 gagctggaaa aggcgcgcgg tgcgctcgcc tcagcggtcg gcttgccagt taatgcaacg
    10261 gtcaaagtca tgaccataga aagtccaccg cgcattcagg agatcaccac gtcgctcacg
    10321 gaaattctga atcgcgccaa ggcaaatcgt ccggacctgg ttgcggccga ggcgcaggcg
    10381 cgttccgcac gcgcgctggc ggatgcgact gctaggagca ccttgccttc aatctcggtc
    10441 aatgcgacga ccggacgaac cttttacaat aaccgtgcct atacggacac caacagcatt
    10501 ttgctcaatc tgagtatccc gatcttcacc gggttcaact atacctactc cgtgcgccag
    10561 gccgaggcgc gggcagaaca ggccgaggca tctcgcgacg tgctctaccg gcagaccgag
    10621 atggatgtct ggcagtcgta ctatgatgtg cagacggcgg tcgggggggt cagcacgacg
    10681 gaagtccagc tccgggcggc gcagcaggcg gcagaggcga ccctggcgcg ctaccaggcg
    10741 ggttttggat cgttgctgga cctcatcacg gcgcaggtgg aggaatcgaa cgcgcgcgtg
    10801 ctccggatcc agtcctatct cgactggttc accgcggttg cccggctgaa ttactcgatt
    10861 ggcgccagcg acaagagtac ttacgtggtg cagacaagat gaaacgactc tggatggcag
    10921 tgatgttcat cgctatcgcc gcgggcagct atggcgcctg gtggtggtgg cagcagagcg
    10981 gcgcgtctgc agccgagggc aggagcgcga aagccaaggg caaaggcaag ggcgctcccc
    11041 tcttcgtgaa gacgaccagg gcggtggcga agccgatgcc ggtattgatc gaggcggtcg
    11101 gcacggtcga gtcggagcat agcgtgcaga tcagggcgca ggtgtccggt gtgctggaga
    11161 aagtactgtt caaggagggc gacaaggtca aggccgggca gctgctgttc cagatcgatc
    11221 cgcgcacatt cgaggcggct taccggcagt cccaggcgca gctggcgcgc gacatggcgc
    11281 agctcgagaa cgccaaggtg caacaggacc gtctcgagcc gctgctcaag cgtgagttca
    11341 tcacgcgcca ggaatttgat gtcgcggtga cttcgaccaa gtcgctcgag gccaccgtcg
    11401 ccgcggacca ggcactgatc gagcaggcgc ggattcagct ggaattttcg cggattcacg
    11461 cgccgatcac cgggcgcacc ggaacgctgg cgatcaagcc gggcaacctg gtgcccaccg
    11521 cgggcggcgg agtgccgctc gtgaccatca acagtaccga ccccatcctc gtgtcgttca
    11581 gcatcccgga gcgtcagctg gaggaaatcc ggcgttatca aaacgacaag gacatgagga
    11641 tcgaaatact gcccgaccgc aatggtccgt cagtggcgga tgggaaactg gtgttcatcg
    11701 acaataccgt gacgccgcag accggaaccg tgctgctcaa gacccgcgtt cagaacaagg
    11761 aggagctgct gtggccagga caattcgtca acgtccggac cgtgctcaag atcgagctgg
    11821 aggcagtggt ggtgcccgaa gtggcggttc agcccggcca gcagggcaac tttgtctaca
    11881 tggtcgagga cggcagggcc aagatccagc cggtgagggt ttcgcgacaa atcggcgggg
    11941 aagtggtcat cgccgaggga atcaagaccg gtgatatcgt gttgaccgag ataccgcagg
    12001 caatcgcgcc cggtgcccag gtgcagatct cagacgcgga aggggcgggc aaggggcgtg
    12061 acggaaaagg caaaggaagg ggcggcaaag gcaggtcgga taaaggcgaa gccaaaagct
    12121 gacgttaccc cgagagcatc ctgacatgaa caccaacctc tcgcgcatct tcatcgagcg
    12181 acccgtcgcc acctcggtgt tggtgatggc ggttgtcttt ttcggaatga tcgcgttccg
    12241 cagcctgccg gtgaacgatc tgcccaacgt cgatttcccg accatccagg ttacggccaa
    12301 tttgcgcggc gccaacccgg aagtgatggc ctctacggtc gcaacgccac tcgagcggca
    12361 gttcagccgg atcgccggtc tcgatacgat gagctcgaac agtaccaacg gcgtcacgcg
    12421 cgtcacgctc cagttcagtc tggatcggag catcgattcc gcggcgcaag acgttcagac
    12481 ggcaatcacg caggcgatgc gattcctgcc ggccgatgct gatccgccct atctgcgcaa
    12541 ggtaaatccg gccgatgcgc caatcctgtt cattgcggtc acggcgcaga cactgcccct
    12601 taccaagctc gatgagttcg ccgacaacca cattgcgcag cggttttcca tggtgagcgg
    12661 cgtggcgcag gtttccgtat tcggatctgc gaagtacgcg gtacgcgtct atgtcaatcc
    12721 ggaatcgctg gcgaagcgcg ggcttgggct cgacaaggtg gtgagcgcga tccagaacgc
    12781 caacagcaat cttccgtccg gcgtgatgtc tggcgccgcg cgcaattaca ccgtgaagtc
    12841 atccggcaag ctggatcgcg ctgcgagctt cagtgatctc atcgtcgcct attccaacgg
    12901 gatgccggta cggttgtccg acatcgggcg tgccgaagac agcgtggaga acgtcaagaa
    12961 cgcgagctgg gtaaacggtg agcgagcgat ctacttcgcg gtcttccgcc agccgggctc
    13021 gaatacggtt gaagtcgtac gcaatatccg cgcattgttc ccggagatcg agcgcgaagc
    13081 gccgccggga gtcaagatgc acgtgctcaa tgatcgctcg gtgttcatca gcgcctcgat
    13141 tcatgatgtg aacttcacgc tcgtgttatc gatgattctc gttgtgctgg tgatcctggt
    13201 gttcctgcgc aacgcgactt cgaccctgat caccgcactt gttctcccga cctcggtggc
    13261 cggcactttt gccgcgatgt acctggtcgg gttcagcctc aacaacctgt ccttgatggc
    13321 tatcattctc gcggtcggtt tcgtggtgga tgacgcgatc gtggtgctgg agaacatcac
    13381 gcgccacatg gagatgggca aggacaggat gcgcgccgcc ctggacggct ccaaggaaat
    13441 cgcgttcacc gtgctgtcca tgacgatttc gctcgcagcg gtatttctcc cgatcctgtt
    13501 catgggcggc atgctcgggc gactgttccg cgagttcgtg gtgactgtgg gggccgcggt
    13561 tctgatatca ggcgcaattt ctctgtcgat gacgccgatg ttgtgcagtc tgatgttgcg
    13621 gcccacgcat gagcacggcc gcgtctaccg gtggctggag cgcatattcg acatagcgcg
    13681 cgatgcctac ggcgcaagcc tgtgctggac catgcgccat cgcgggctga tgctggccgg
    13741 atcggggatc gtgcttgtcc tgatcttcgt cgtctacaag atggtgccac aaggcttcat
    13801 cccgcgtcag gataccggct tgttcttcgg caacatccgt gcgcccgagg gcgtgacttt
    13861 cagcgagctc gtccggcgtc agaagttggt ggccgacata atacagagca acccgaatgt
    13921 ggaagcagtg ctttccaccg ccggccaggg agtcggggga gtcgtcggag acaacgttgg
    13981 tcgcgtggtg gtccggctca agccgcgcaa cgagcgcaag ctcagtgcgg acgaggtgat
    14041 tcaggaactg aggcgcgcct ttgccgggag cccgcaaggc ctgcgtgtct tcctgaacaa
    14101 cccgccggca atcaatatcg gcgggctgat cagcaccgct gactaccagc tggtgatcca
    14161 ggccacggag ttggaaacgc tgtatcaacc ggcgcaggag ctggaaggga aactgcgcca
    14221 gctcggagtg gtcacggatg tcagctccaa tcttgagctg cgtaatcccg agatccaagt
    14281 gtcgatcctt cgtgaccggg cggcggcgct aggcgtgaat ccgcagcaac tcgaaacgac
    14341 tctctacaac gcctatggcg gtcgccagat cagcacgctc tatggggcga ccgatcagta
    14401 ttacgtgatt ctcgaactcg atccgcgatt ccaacgcaac atcgacgcgc tgcgctcgct
    14461 ctacattcag agcaactcgg gacagatggt cccgatgcat gccgtggccg acatcaagag
    14521 cggagtcggc ccggtatcgg tcacccatta cggccagctg ccatcggtga tcctgtcgtt
    14581 caaccttgcc ctaggcgcct caatcggcca agcggtggat agcgttcagg aagtcacgcg
    14641 cgtactgccg agcggcatca catcgctgtt cgtgggcagc gccaaggctt tcgaggatgc
    14701 gttccgcacg ttgcccatcc tgctgctgat caccatcatc gtgatctaca tgatactggc
    14761 catcctctat gagcattatg gtcatccgat cacgatcttg acggcgctgc cattcgccgg
    14821 cttcggggcg ctgttgatgc ttctcctgtt caatatggag ctgaacatat tcagcttcgt
    14881 cggcatcatt ctgttggtcg gcctggtgaa gaagaacggc atcatgatgg tggacttcgc
    14941 gctgcagctt caacgcgata aggggctgtc agcacaggac gcgatcgtgg aagcctgcct
    15001 catacgcttc cgcccgatca tgatgaccac gatgtcggcg atattcgcga cgcttccgat
    15061 tgcgctgggt tacggcgcag gcgccgagac ccgccgtccg ttgggtgtgg cggtggtggg
    15121 tggcttgctg ttttcgcagt ttctgaccct ttacgtgacg cccaccttct acgtcagcat
    15181 ggaacgcttg atcgggtact ttcggcgcca tcgtgcggtg gaagccgttg gacgctgaac
    15241 gtgccatacc gacgccgtgt tgacgcaagg tggcgattcg ggcgcgccgt tcgcgtcaag
    15301 gcacaggtta ttgaaaaaag gtggaaaacg agttgactta gtcaccctca acccgctaca
    15361 atcccaagtc tgattcgagc gcggggtgga gcagtctggc agctcgtcgg gctcataacc
    15421 cgaaggtcat aggttcaaat cctatccccg ctaccagatc ccggaacccg gtggatgctt
    15481 cccccggttt cccctaagtc aggccgcaga gattctgcgg taccccttag aaacgttctt
    15541 taacaatcta cagccgataa gtgtgggcac ttggtttccg ggggcccgcg gcgctttcgc
    15601 gtacgcggaa tctcgatgca accgaagtgc tcgcacgacg taacatacgt caagcgagtt
    15661 aatactttgc agtgattaaa ctgaagagtt tgatcctggc tcagattgaa cgctggcggc
    15721 atgccttaca catgcaagtc gagcggcagc gcgggggcaa ccctggcggc gagcggcgaa
    15781 cgggtgagta atacatcgga acgtgtccag tagcggggga taacctgtcg aaagataggc
    15841 taataccgca tacgacccac gggtgaaagc gggggaccgc aaggcctcgc actattggag
    15901 cggccgatgt cggattagct agttggcggg gtaaaagccc accaaggcga cgatccgtag
    15961 ctggtctgag aggacgacca gccacactgg aactgagaca cggtccagac tcctacggga
    16021 ggcagcagtg gggaattttg gacaatgggc gcaagcctga tccagccatg ccgcgtgagt
    16081 gaagaaggcc ttcgggttgt aaagctcttt cggcggggac gaaacggtcc gtgccaacac
    16141 cacggactaa tgacggtact cgcagaagaa gcaccggcta actacgtgcc agcagccgcg
    16201 gtaatacgta gggtgcaggc gttaatcgga attactgggc gtaaagcgtg cgcaggcggc
    16261 tccttaagtc agatgtgaaa tccccgggct taacctggga actgcgtttg aaactggggg
    16321 gctggagtgt ggcagagggg ggtggaattc caggtgtagc agtgaaatgc gtagagatct
    16381 ggaggaacac cgatggcgaa ggcagccccc tgggctgaca ctgacgctca tgcacgaaag
    16441 cgtggggagc aaacaggatt agataccctg gtagtccacg ccctaaacga tgtcaactgg
    16501 ttgtcgggga agtaattcct tggtaacgaa gctaacgcgt gaagttgacc gcctggggag
    16561 tacggccgca aggttaaaac tcaaaggaat tgacggggac ccgcacaagc ggtggattat
    16621 gtggattaat tcgatgcaac gcgaaaaacc ttacctaccc ttgacatgtg cggaatgggg
    16681 tagagatacc ttggtgcccg aaagggaacc gcaacacagg tgctgcatgg ctgtcgtcag
    16741 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttgt cattagttgc
    16801 cacattcagt tgggcactct agtgagactg ccggtgacaa accggaggaa ggtggggatg
    16861 acgtcaagtc ctcatggccc ttatgggtag ggcttcacac gtaatacaat ggccggtaca
    16921 gagggctgcc aacccgcgag ggggcgctaa tcccagaaag ccggtcgtag tccggattgt
    16981 tctctgcaac tcgagagcat gaagtcggaa tcgctagtaa tcgcggatca gcatgtcgcg
    17041 gtgaatacgt tcccgggtct tgtacacacc gcccgtcaca ccatgggagt gggtttcacc
    17101 agaagtagtt agcctaaccg caaggagggc gactaccacg gtgagattca tgactggggt
    17161 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctagagat
    17221 ccgcctccgg cgccaggtgt ccactcttat cggttgtatt tcggacgctg tcagctctca
    17281 gctttcagca atcagcgtga aaatgcggtt tgtggaggtt tggctgacgg ctgggtgctg
    17341 agtgctgacc gctgaaaggg gtctgtagct cagctggtca gagcacacgc ttgataagcg
    17401 tggggtcgtt ggttcaaatc caaccagacc caccaagcct tatcgggggt gtagctcagt
    17461 gggagagcac ctgctttgca agcagggggt cgtcggttcg actccgatca cctccaccaa
//
LOCUS       DBNGWZ010000638         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1747223, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000638 DBNGWZ010000000
VERSION     DBNGWZ010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1747223"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16CX_20055"
     CDS             complement(<1..184)
                     /locus_tag="AC16CX_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3014835.1"
                     /translation="MPAGVNVVILAAGQGKRMRSRLPKVLHPLAGRPLIAHVVETARA
                     LKPARICVIYGHGGEQV"
     gene            complement(206..1699)
                     /gene="glpD"
                     /locus_tag="AC16CX_20060"
     CDS             complement(206..1699)
                     /gene="glpD"
                     /locus_tag="AC16CX_20060"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR3014836.1"
                     /translation="MATVPNSVDLLVIGGGINGAGIARDAAGRGLSVLLVDKDDLGAH
                     TSSSSSKLIHGGLRYLEHFEFRLVAEALEEREVLLRVARHLVWPARFVMPHVPQLRPR
                     WMIRTGLFLYDHLARRASLPGSHAVRLDAPPYNAGLRPQFKHGFIYSDCRVDDARLVI
                     INAMDARRRGATILTRTECLGARRQGDGWHATLSGGREIRARAIVNAAGPWVKRVLNE
                     RLGQPSRDGVKLVKGSHIVLPQLYPGDHAFILQNDDRRVVFMIPYGDLHTLVGTTDVP
                     VEGDPGAALASDAEAAYLCRAVGRYLEKPVEPAQVAWRFAGVRPLYDDGTSDPSSITR
                     DYTLRVDDASGTAPALSVYGGKITTYRRLAEHAMQKLAQYFPGLKGNWTAGEPLLGGD
                     FAGASREQARDELFARYDGLPQEMLRGLFRRHGMLAYEVLGDAASVTDLGEHFGADLY
                     AREIDYLVEHEWARTAEDVLWRRTKAGLHLAPEQREVVAQYLARCVS"
BASE COUNT          303 a          630 c          587 g          285 t
ORIGIN      
        1 gcacctgctc gccgccatgc ccgtaaatca cgcagatgcg cgcgggcttc aacgcgcgcg
       61 cggtctcgac cacgtgcgcg atgagcgggc gtcccgccaa ggggtgcagc accttgggca
      121 ggcgcgagcg catgcgcttg ccctggccgg cggcgagaat aacgacgttg actccggctg
      181 gcatggggaa attatcgcac aaacatcaac ttacgcaccg agccagatac tgcgcgacca
      241 cttcgcgctg ttccggggcc aggtgcagtc cagccttggt gcgacgccac agcacgtctt
      301 ctgcagtgcg cgcccattca tgctcgacca gatagtcgat ctcccgcgcg taaaggtcgg
      361 ccccgaaatg ctcgccgagg tccgtgaccg aagcggcgtc gcccagcacc tcgtaggcga
      421 gcatgccgtg ccgcctgaac aggccgcgca gcatctcctg cggcaggccg tcataacgcg
      481 cgaacaactc atcgcgcgcc tgctcgcggc ttgcgcccgc gaaatcgccg cccagcagcg
      541 gctcgcccgc agtccaattc cccttcagcc ccgggaaata ctgcgcgagc ttctgcatgg
      601 cgtgctccgc aaggcgccgg taggtcgtga tcttaccgcc atacaccgac aacgccgggg
      661 ctgttcccga tgcgtcgtct acgcgcaagg tgtagtcgcg cgtgatcgac gagggatcgc
      721 tcgtgccgtc gtcgtagagc ggccgcacac cggcaaagcg ccaggcgacc tgcgcgggtt
      781 ccaccggctt ctccaggtag cgccccacgg cgcggcacag gtacgcggct tcggcatcgc
      841 tcgccagcgc cgcgcccgga tctccttcca ccgggacatc ggtcgtgccg acgagcgtgt
      901 gcagatcgcc gtagggaatc atgaacacga cgcgccggtc gtcgttctgc aggataaacg
      961 catgatcgcc cgggtagagt tgcggcagga cgatatggct gcccttgacg agcttgaccc
     1021 cgtcacggct cggctggccg aggcgctcgt tcagcacgcg cttcacccac gggccggccg
     1081 cgttcacgat cgcgcgggcg cggatctcac gacctccgga aagcgtcgcg tgccagccat
     1141 ctccctggcg ccgcgcgccc aggcactcgg tccgggtcag aatcgtggcg ccgcggcggc
     1201 gcgcgtccat cgcgttgatg atgaccagcc gcgcgtcgtc cacgcggcaa tcggaataga
     1261 tgaagccatg tttgaactgg ggccgcagcc cggcgttgta gggtggggca tctaggcgta
     1321 cggcgtgcga gccgggcagg ctcgcgcgcc gcgccaggtg atcgtagagg aagagcccgg
     1381 tgcggatcat ccagcgcggc cgcaactgcg gcacgtgcgg catgacgaag cgcgccggcc
     1441 acaccagatg ccgggcgacc ctgagcagca cctcgcgctc ctcgagcgcc tccgcgacca
     1501 accggaactc gaaatgctcc aggtagcgca ggcccccgtg gatgagcttg ctgctggatg
     1561 agctggtatg cgcgccgagg tcgtccttat ccaccagcag cacggacaga ccccgtccgg
     1621 cagcgtcgcg cgcgatgccg gcgccgttga tgccgccacc gatgaccagc aagtcgacgc
     1681 tattgggtac tgtcgccata aatgaaaagg gcagcccttg cgctgcccta tattttgctc
     1741 tcccgcgagt tgaacggccc tagggttctg gagccgcggc tcccgtgccc taaggcgtcc
     1801 gttcg
//
LOCUS       DBNGWZ010000639         4444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_360940, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000639 DBNGWZ010000000
VERSION     DBNGWZ010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_360940"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1660)
                     /locus_tag="AC16CX_20065"
     CDS             complement(284..1660)
                     /locus_tag="AC16CX_20065"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3014837.1"
                     /translation="MDKEQVQERRDFLKQFSATLGAAGLGAAGIGTAHAAVKPKGNIP
                     NRPVKVGHITLLTGPAELLGGPGQRGHVLAQEEINAEGGLLGKRKIETIFADEAAGTD
                     ANVKELRRMKLSEKIELFTGVISSGNTPALGPVAEELKVLTLFNDGCTDFLFDKAVPN
                     PKYAFRVTNIQSADGVTAAIGAATHWPKVRRIASLQPDYSYGRNVFEHFVLALGKLLP
                     GIEVVSESWPKLGTTDFTSHITKILSTKPDLLIVGVWGGDYVAFYKQALRYGLFDKMK
                     VATTLAFGGAPQAIGKDHPEGVLAGVHANYHFLYPSAERWPVNKGFVERYHKRWKEYP
                     SFESDGAYTALYLYKQAVEKANQLAGGWPNDEAIIAMLEGAAIDSPAGYLHIRADNHQ
                     GYKDAVTGLSKNLPGYDFPVWEPASVVSIPIRSITAPPNWPKPGQGHNESTAAADWIK
                     KTWPQAKA"
     gene            complement(1822..3999)
                     /locus_tag="AC16CX_20070"
     CDS             complement(1822..3999)
                     /locus_tag="AC16CX_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014838.1"
                     /translation="MNYFILGAGLALLGVAHAGHEFPFYPSFYPQEITVEALDAHAAA
                     RRIAKGTLHAYAGGELAVKSDPAKTGTVASLGGYVVVTFDRGAPQFADSSARCAAARG
                     LKHTLTEGQVWHPYPVTPFHADYLYHADRAEAARAAVAAQKIAKPPGRLEIIDMTVLA
                     AQAGARYNGWPGPPWLRQGWFHAYLLLAPAVSDTAARARIEDAARRLMRGEHRSLLER
                     IERERELVALLQSGCERIVLGYTVRRERYSTEYSQGVENVGYDALDGLASAIFPRTVK
                     LRDFPWNGWLNVAVPALPSSAWNPVAGGFGDPFGRLVWWALGDPAFLPSPHGEGWIEN
                     RLSASDAKRGTPLAVPEDALLPEPGSGRLAPVGRGRSATTRIVYRVRNSAFHDGTPMS
                     VADLFYAYAFAAAWGAGDAADPGVAQATALARERLKGVRLLRVETESLAFGEDKLSYE
                     VPVIEVYLDAGTAGDAATVAPPWTIVPWHLLALFEEGVRRGYFAFSSAGAGRRGLPEL
                     DPVRNAAIVRRLAELARELEERAYVPAALAPYVGAGEARQRYSRLREFHAAHGHWLVT
                     NGPYVLNRWDTTKAVLGVFRDPSYPKGLGSFNAYAAPLKAHVTRVERRSYGAEVHTEI
                     EWLERLGRDVRIVRGSFAQRLAERLTTAGPPPAPVCRYLLVGADGAVAAAGTARADAT
                     GTCRLEFARPGRRASGGSDRLALAAVLEDNSANALIKFVPWEH"
     gene            4030..4404
                     /locus_tag="AC16CX_20075"
     CDS             4030..4404
                     /locus_tag="AC16CX_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014839.1"
                     /translation="MAARIVASVAVLGACLALAVMAADEGAQGTTRVFELRIAQRLVP
                     LAQRVLRATEGERVELRWTAEEALVLHLHGYDIETRVEPGKPAITAFVARLTGRFPVA
                     IHAVGGSTHRHSALLHVEVHPR"
BASE COUNT          697 a         1511 c         1481 g          755 t
ORIGIN      
        1 cattcgacct cgtcggccag gttttttgct gaatgatcag ccggttttgc tgttagcagc
       61 ctgtgcgggc tgttaagccc gacaggctgc tagcgtagtc atccccacaa tccgtgcggc
      121 gccgcacagc ggcgaattcg cgacgcaagg cgctcggcgg cgcacaccac gatcgatgtg
      181 ttcgcggtcg aggcggcggt tctgttttcc ccgtgcgcac cgccggaacg gctcggcctt
      241 gcgaggccgg gaccgccccg gatgcgcgcc gaaggctagc gcgtcacgcc ttcgcttgcg
      301 gccaggtctt cttgatccag tcggccgcgg ccgtggattc gttatgcccc tggccgggtt
      361 tcggccagtt gggcggcgcg gtgatgctgc ggatcgggat cgagacgacg ctcgccggtt
      421 cccacaccgg aaaatcgtat cccggcaggt tcttgctcag gcccgtcacc gcgtccttgt
      481 agccctggtg gttgtcggcg cggatatgga ggtagcccgc tggcgaatcg atcgccgcac
      541 cctcgagcat ggcgatgatc gcctcgtcgt tgggccagcc gccggcgagc tggttcgcct
      601 tttccaccgc ctgcttgtag aggtagagcg cagtgtaggc gccgtcggac tcgaagctcg
      661 ggtactcctt ccagcgcttg tggtagcgct cgacgaagcc cttgttgacc ggccagcgct
      721 cagcagaagg atagaggaag tgatagttcg cgtgcacgcc ggcgagcacg ccctcggggt
      781 gatccttgcc gatcgcctgc ggggcgccgc cgaaggcgag cgtggtggcg accttcatct
      841 tgtcgaacag cccgtagcgc agcgcctgct tgtagaacgc gacgtaatcg ccgccccaca
      901 cgccgacaat cagcagatcg ggcttggtcg aaaggatctt cgtgatgtgc gacgtgaaat
      961 ccgtggtgcc gagcttcggc caggactccg aaacgacctc gattcccggc agcagcttgc
     1021 cgagcgcgag cacgaagtgc tcgaagacgt tgcgcccata gctgtagtcg ggctggagcg
     1081 aggcgatacg ccgcaccttg ggccagtgcg tcgcggcgcc gatggcggct gtgacgccgt
     1141 ccgcggactg gatgttggtc acccggaacg cgtacttcgg attcggcacc gccttgtcga
     1201 agaggaagtc ggtgcagccg tcgttgaaca gcgtgagcac cttcagctcc tccgcgaccg
     1261 gccccagcgc cggagtgttg ccgctcgaga tcacgcctgt gaagagctcg atcttctccg
     1321 agagcttcat gcgccgcagc tccttcacgt tggcgtcggt gccggcagct tcgtcggcga
     1381 agatcgtctc gatcttgcgc ttgccgagca gcccgccctc ggcgttgatt tcctcctgcg
     1441 cgaggacatg ccctcgctgg cccgggccgc ccagaagctc ggccgggccg gtgagaaggg
     1501 tgatgtgacc gaccttgacc ggcctgttgg ggatattgcc cttcggtttc accgcggcat
     1561 gcgcggtacc gattcccgcg gccccgaggc ccgcagcgcc cagcgttgca ctgaactgtt
     1621 tcagaaaatc gcgacgttcc tgtacctgtt ccttgtccat ggctctcctc cttttaacaa
     1681 gcgaacgaaa tttcacgttt gctccagcga gcccgttcgg acagccgggc tgtcaggccc
     1741 caccgtccac gggttcgcac aacgcggtgc ccgggatcaa gtcgcgcgac gtgcaccgca
     1801 cgacggtacg cgcgaaatcg attagtgttc ccacggcacg aacttgatca gcgcgttggc
     1861 ggaattgtcc tctagcaccg cagcgagcgc aagccggtcg ctgcctccgc tcgcccgcct
     1921 ccccgggcgt gcgaattcca ggcggcacgt gccggtggcg tccgcgcgcg ctgtccccgc
     1981 ggccgcgacc gcgccgtccg caccgacgag caggtagcgg caaacgggtg ccggcggcgg
     2041 gcccgcggtc gtgagccgct cggcgagtcg ctgcgcgaac gagccgcgca cgatcctcac
     2101 atcgcgcccg aggcgctcga gccactcgat ttcggtatga acctccgcac cgtaactgcg
     2161 ccgctcgacc cgcgtcacat gcgccttgag cggcgccgcg taggcgttaa agctgcccaa
     2221 tcccttcgga tagctcggat cgcggaagac acccagcacc gcctttgtcg tgtcccagcg
     2281 gttcagcacg taagggccgt tggtcacgag ccaatgtccg tgcgccgcgt gaaactcgcg
     2341 caatctgcta tagcgctggc gcgcttcgcc ggcgccgacg tacggcgcga gcgcggccgg
     2401 aacgtaggcg cgctcttcga gttcgcgggc aagctctgcg agccggcgca cgatcgccgc
     2461 gttgcgcacc ggatcgagct ccggaagacc gcgacgcccg gcgcctgccg aggagaaagc
     2521 gaagtacccc cggcgaacgc cttcctcgaa cagcgccagc aggtgccagg gaactatcgt
     2581 ccacggcggc gcaaccgtcg ccgcgtcgcc cgcggttccg gcgtcgaggt agacctcgat
     2641 caccgggacc tcataactca gcttgtcttc gccgaacgcg aggctctcgg tctccacgcg
     2701 caggagccgc acgcccttga ggcgctcacg cgcaagggcg gtcgcttgtg ccacgcctgg
     2761 atccgctgca tcgcccgcgc cccacgcggc cgcaaaagca taggcataga acaggtccgc
     2821 gacgctcatc ggcgtgccgt cgtggaacgc cgagttgcga acgcggtaga cgatgcgcgt
     2881 cgtggccgac ctgccgcggc ccacaggcgc gagccgcccg ctgcccggct caggcagcaa
     2941 ggcatcttcg ggcaccgcga gcggcgtccc cctcttcgca tccgacgcgc tcagccggtt
     3001 ctcgatccag ccctctccgt gcggcgatgg caagaacgcc ggatcgccaa gcgcccacca
     3061 aacgagtcgc ccgaaggggt cgccgaaacc gccggcgacg gggttccatg cgctggaagg
     3121 cagtgccggc accgcgacat tgagccagcc gttccaggga aagtcccgca gcttgacggt
     3181 ccggggaaag attgccgaag ccagaccgtc gagcgcgtcg tagccgacgt tctccacccc
     3241 ttgcgagtac tcggtgctgt agcgctcgcg gcgcacggtg tagccgagca cgatccgctc
     3301 acagccgctt tgcagaagag caacgagctc gcgctcgcgt tcgatccttt ccaggaggct
     3361 gcgatgttcg ccgcgcatga gacggcgcgc ggcatcctcg atccgcgcgc gggcggccgt
     3421 atcgctcacc gcgggcgcga gcaggaggta agcgtgaaac cagccttgtc tcaaccacgg
     3481 cggtccgggc cagccgttgt aacgggcgcc cgcctgggcc gcgagcacgg tcatatcgat
     3541 gatctcaagc ctgccgggcg gcttcgcgat cttctgcgcc gcgaccgcag cccgcgcagc
     3601 ttcggcgcga tcggcgtggt acaggtagtc ggcgtgaaag ggcgtaacgg ggtacggatg
     3661 ccacacctgg ccctcagtca gggtgtgctt gaggccgcgc gcggccgcgc agcgcgcgga
     3721 gctgtcagcg aactgcggcg cgccgcggtc gaaagtcacg acgacgtagc cgccgagtga
     3781 agcaacggtg cccgtctttg ccggatcgga cttgacggcg agttcgccac ctgcataggc
     3841 gtgcagcgtc cccttggcga tccgccgcgc ggccgcgtgc gcatcgagcg cttcgaccgt
     3901 gatctcctgc gggtagaacg acggataaaa cgggaactcg tgaccggcgt gcgcgacgcc
     3961 gagcagcgcg aggcccgctc cgagaatgaa gtaattcatc cgcgagcctg atgctacaat
     4021 cacgcggtgg tggcagcaag gattgttgcg tctgttgccg tgctcggcgc gtgccttgca
     4081 cttgctgtca tggccgccga cgaaggcgcg caaggcacca cccgcgtgtt cgagcttcgc
     4141 attgcgcagc gtctcgtgcc gctcgcgcaa cgcgtgttgc gcgccactga gggcgagcgc
     4201 gtcgagctgc gctggactgc tgaagaggcg ctcgtcctgc accttcacgg ttacgatatc
     4261 gagacgcgcg tcgagccggg caagcccgcg atcacggcgt tcgtggcgcg gctgacaggc
     4321 cgctttcccg tggcgatcca tgccgtgggt ggctcgacgc accgccatag cgcgcttctg
     4381 catgtcgagg ttcatccgcg ttagcgcagt ggcgttcgcg gctgcgcccg ccgaggcggc
     4441 ggcg
//
LOCUS       DBNGWZ010000640         6069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_614471, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000640 DBNGWZ010000000
VERSION     DBNGWZ010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_614471"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1530
                     /gene="glnA"
                     /locus_tag="AC16CX_20080"
     CDS             118..1530
                     /gene="glnA"
                     /locus_tag="AC16CX_20080"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR3014840.1"
                     /translation="MSTAADVLKMIKDNEVKFVDLRFTDTRGKEQHVSVPVSAFGEHK
                     FSDGHYFDGSSIAGWKGIEASDMVLVPDPDTARMDPFTDEPTLLLTCDVIEPSDGKGY
                     DRDPRSIAKRGEAYLKSTGVADTAYFGPEPEFFVFDSVTWKVEMGQAFYKIKSEEAPW
                     SSGEEFEGGNMGHRAPIKGGYFPVPPVDSLQDIRSAMVLAMQEMGVECEVHHHEVGAM
                     GQNEIGTKFASLVKRADWMMIYKYAVHNVAHSYGKTATFMPKPVVGDNGSGMHVHQSL
                     AKDGKNLFTGNGYAGLSELALHYIGGILKHAKALNAITNPGTNSYKRLVPGFEAPINL
                     AYSSRNRSAAVRVPLVSNPKSRRIEVRFPDPTTNPYLAFTAMMLAGLDGIQNKIKPGD
                     PIDKNLYDLPAEIALRVPKVCHSLEQALEELEKDHEFLLRGGVFSKDMLNAYIALKEE
                     ELTAFRMQTHPLEFQLYYSL"
     gene            1864..2328
                     /locus_tag="AC16CX_20085"
     CDS             1864..2328
                     /locus_tag="AC16CX_20085"
                     /inference="COORDINATES: protein motif:HMM:NF024901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HIR3014841.1"
                     /translation="MRNHWILAVLLVLPAPPVAADIWECVDESGNKRFTNIKSEAKGC
                     KLLNISAPNTVPATKPQAKPADAPRNFPKVDGETQKQRDSDRRRILEQELASEQKLLD
                     QARKELSEQESMRLGSERNYQRVLERLEPYKKKVKLHEDNVVNLRRELASTK"
     gene            2479..3564
                     /gene="glnL"
                     /locus_tag="AC16CX_20090"
     CDS             2479..3564
                     /gene="glnL"
                     /locus_tag="AC16CX_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HIR3014842.1"
                     /translation="MTPAALSGLDLLATAVVVVDAGLAVRYMNPAAENLFEMSSKNVA
                     GHPLNELFAGTAVLEAAIEYARTNNCSYTEHDLELGMNGRARLHLSCTVTPVEFARDD
                     ARGEDLLLEFRHIEQQLRIAREERMLDQSQANRELIRNLAHEIKNPLGGIRGAAQLLD
                     HELERPNLHEYTQVIMKEADRLQSLMDRLLTPHRLPQPARLNMHEVLERVRSLILAEY
                     PQGILIRRDYDISLPLLKGDKEQLIQAVLNVVRNAAQAVMGGGGNGEVRLRTRAVRQV
                     TLARRRYRLALAVEITDSGPGIPPELQERVFHPLVSGREGGSGLGLTLAQNFITQHHG
                     VISFESVPGETTFTILLPVEEAENSKS"
     gene            3623..5020
                     /gene="ntrC"
                     /locus_tag="AC16CX_20095"
     CDS             3623..5020
                     /gene="ntrC"
                     /locus_tag="AC16CX_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR3014843.1"
                     /translation="MKPVWIIDDDRSIRWVFEKALARENIAFKTFSSAQEALAALNTE
                     TPQVVVSDIRMPGESGLELLQRAKALHANLPVIIMTAYSDLESAVTAFQGGAYEYLPK
                     PFDVDHAVELIRRAMEESMRQESVAEETEATPEILGQAPAMQEVFRAIGRLSQSHATV
                     LITGESGTGKELVARALHRHSPRSGRPFIAINTAAIPRELLESELFGHERGAFTGAQA
                     QRRGRFEQAEGGTFFLDEIGDMPQELQTRLLRVLSDGHFYRVGGHQPIKANVRVIAAT
                     HQDLEARVKQGLFRDDLFHRLNVIRLRLPSLRERREDIPLLARHFLARSARELGVEAK
                     RLSGAALKYLVAQDFPGNVRQLENLCHWLTVMAPSVTIEVKDLPPELRTEAAPAAASW
                     MAALEKEVETLLNRGETGVMDELARQFEKTLIVKALAHTGGRRIEASQLLGLGRNTLT
                     RKIQELGIDAEKKNG"
     gene            complement(5105..>6069)
                     /locus_tag="AC16CX_20100"
     CDS             complement(5105..>6069)
                     /locus_tag="AC16CX_20100"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR3014844.1"
                     /translation="PERADTRGIVEPRLECGGMPPVGHPVGAFGHPWRLQFTGTRGII
                     SGVTARYRTELLQTDAPINQGNSGGPLISLETGRIVGINTAGIRGAQNTNFAVAIKYA
                     CRVLQLLQEGLDPSPPDLKLVYFRDVDNRKQLKVARSYAGDGTLKLLPGDIIRQVIGE
                     EGAIENETQLLHALRGRLAGSSLKVLRAGQEVAVSGAKVPMRRVLETRGVYASGILFG
                     PITIRDALEIGARGLMVHHVEPGSIGESREIEKADLLEAVDGAPVLEVDELYNRLSEA
                     RRDSRRVTLTLKKLAGGDTLFSYSQRNLVIADLRIIGPQKSVTE"
BASE COUNT         1179 a         1967 c         1927 g          996 t
ORIGIN      
        1 caaacccctt gtggcacaat gcgtttcgca caaaaaccct tggcacaatt cctgctcaac
       61 aggaagaccc gattcaccgt attttcgttt cacaccaatc aacttcagga gagctagatg
      121 tcaaccgccg ctgacgtatt gaaaatgatc aaggacaacg aggtgaaatt cgtcgacctg
      181 cgcttcaccg acacccgcgg caaggaacag cacgtgtcgg tcccggtatc cgcgttcggc
      241 gagcataaat tcagcgacgg ccattatttc gacggctctt ccatcgccgg ctggaagggc
      301 atcgaggctt ccgacatggt gctggtgccc gatcccgaca ctgcgcgcat ggacccgttc
      361 accgacgagc cgacgctgct gctcacctgc gatgtgatcg agccttccga cggcaagggc
      421 tacgaccgcg atccgcgctc gatcgccaag cgcggcgagg cctacttgaa atccaccggc
      481 gtcgccgaca ccgcatactt cggccccgag cccgaattct tcgtcttcga ctcggtgacg
      541 tggaaggtcg agatggggca ggcgttctac aagatcaagt ccgaagaggc gccatggtcc
      601 tcgggagagg aattcgaggg cggcaacatg gggcaccgcg ccccgatcaa gggcggctat
      661 ttcccggtac cgccggtgga ctcgctgcag gacatccgct cggcgatggt gctggcgatg
      721 caggagatgg gcgtggagtg cgaagtgcat caccacgaag tgggcgccat ggggcagaac
      781 gagatcggca ccaagttcgc gagcctggtg aagcgcgccg actggatgat gatctacaag
      841 tacgcggtgc acaacgtcgc gcattcctac ggcaagaccg cgactttcat gccgaaaccg
      901 gtggtgggcg acaacggctc gggcatgcac gtgcaccagt cgctcgcgaa ggacggcaag
      961 aacctcttta ccggcaatgg ctacgcgggg ctgtccgagc tggcgctgca ctacatcggc
     1021 ggcattctca aacacgcgaa ggcgttgaac gccatcacca acccgggtac caattcgtac
     1081 aagcgcctgg tgcccggatt cgaagcgccg atcaatctcg cgtactcctc gcgcaaccgc
     1141 tcggcggcgg tgcgcgtgcc gctggtgtcg aacccgaagt cgcgccgcat cgaggtgcgt
     1201 ttccccgacc cgacgaccaa cccctatctc gcgttcaccg cgatgatgtt ggcggggctc
     1261 gacggcatcc agaacaagat caagccgggc gacccgatcg acaagaacct ctacgacctg
     1321 ccggcggaaa tcgccttgcg cgtgcccaag gtgtgtcatt cactcgagca ggctctggaa
     1381 gaactcgaga aggaccacga gttcctgttg cggggcgggg tattctccaa ggatatgttg
     1441 aacgcctata tcgcgctcaa ggaggaggag ctcacggctt ttcgcatgca gacccatccg
     1501 ctcgagtttc agttgtatta cagcctttag gaatttgccg gaccgaggtc cggcaaattc
     1561 ctcaggcaga ttccctccga aaaccggggg aggcgggcgg ctcctcagcg gtgcctgtcg
     1621 gaagcagcgg tccggcgggc gccttggagc accaccgctc aagtgttcta atccggtttc
     1681 atatgagttt ccgttcagga atttgtcggg acagcacccc gacaaattcc tccttgcacc
     1741 gtttaggtgc gttgcaaaat ctgttttatt gacatagact taggttagct atcgagaccg
     1801 accggtcaac atcaggtgcc cgcgaaacgt tatcggtcag taacaacgag gttccgacgg
     1861 atgatgcgca accactggat tctggccgtg ctgctcgtgc ttcccgctcc cccggtggca
     1921 gcggacatct gggagtgcgt ggacgagagc ggcaacaagc gcttcaccaa catcaagtcc
     1981 gaagcgaaag gctgcaagct gctgaacatc tcggcgccca acaccgtgcc ggccacgaaa
     2041 ccccaggcca aaccagccga cgccccgagg aatttcccca aggtggacgg cgaaacgcaa
     2101 aagcagcggg atagcgaccg ccgcaggata ctcgagcagg agctcgccag cgagcagaag
     2161 ctcctggacc aggcgaggaa agagctctcg gagcaggaat cgatgcggct gggcagcgag
     2221 cgcaattacc agcgcgtgct cgaaagactc gagccctaca agaagaaggt caagctgcac
     2281 gaggataacg tcgtcaacct gaggcgcgag ctggccagca ccaagtaagg ttgcggcccg
     2341 gcgcccggtg acaccgatgt atgcggctga cggggaattt atcccctggg tgcgcagcat
     2401 tcctgccacg actttatggt caggcgcctg ccgtccttgg cgcggttctt gctccaatcc
     2461 cgtacccgga cctgtgacat gacaccagcg gctctctccg gtctcgatct cctggcgacc
     2521 gcggtggtgg tggtggatgc ggggcttgcc gtgcgctaca tgaatccggc ggccgagaac
     2581 ctattcgaaa tgagcagcaa gaacgtggcg ggccatccgc tcaacgaact gtttgccggg
     2641 accgcggtgc tcgaagccgc gatcgagtac gcacgcacca acaattgcag ctacaccgaa
     2701 cacgatctcg agctcggcat gaacgggcgc gcgcggctgc acctgtcatg caccgtcacg
     2761 ccggtcgaat tcgcccggga cgacgcgcgc ggcgaggatc tgttgctcga attccgccac
     2821 atcgagcagc agctgaggat tgcccgcgag gagcgcatgc tcgatcagag ccaggccaat
     2881 cgcgagttga tccgcaatct cgcccacgag atcaagaacc cgctcggcgg catccgcggg
     2941 gcggcgcagc tcctcgacca cgagctggaa cgtcccaacc tgcacgaata cacgcaggtg
     3001 atcatgaagg aggccgaccg cctgcagtcg ctgatggacc ggctgctcac gccgcaccgg
     3061 ctgccgcagc cggcccggct caacatgcac gaggtgctgg agcgggtgag gagcctcatc
     3121 cttgccgagt atccgcaggg catcctcatc cgccgcgatt acgacatcag cctgccgctg
     3181 ctgaaaggcg acaaggaaca gctcatccag gcggtgctca acgtcgttcg caacgcggcg
     3241 caggccgtca tgggcggcgg cgggaacggg gaagtccggc tgcgcacccg cgccgtgcgc
     3301 caggtgacgc tggcgcgccg ccgctatcgc ctcgcgctcg cggtggagat taccgacagc
     3361 ggccccggca tcccgccgga gctgcaggaa cgcgtgtttc accccttggt gtcgggccgc
     3421 gagggcggca gcggactggg gttgacgctg gcgcagaact tcatcaccca gcaccacggc
     3481 gtcatttcct tcgagagcgt gcccggggag acgacgttca cgatcctgct ccccgtcgaa
     3541 gaagcagaga acagcaaatc gtgaatggag ggaacatgac ggtttactcg aatcacgaat
     3601 cacgactcac gactcacgcc tcatgaaacc ggtgtggatc atcgacgacg accgctcgat
     3661 ccgctgggtg ttcgagaagg cgctcgcgcg cgagaacatc gcgttcaaga cgttttcctc
     3721 ggcgcaggag gcgctcgccg cgctcaacac cgagacgccg caggtggtgg tgagcgacat
     3781 ccgcatgccg ggggaatcgg gcttggagct cttgcagcgg gcgaaagcgc tgcacgccaa
     3841 cctgccggtg atcatcatga ccgcctattc cgacctggag agcgcggtta ccgcgttcca
     3901 gggcggcgcc tacgaatacc tgcccaagcc gttcgacgtg gaccacgcgg tggagctgat
     3961 ccggcgcgcg atggaagaaa gcatgcgcca ggaaagcgtg gccgaggaaa cggaagccac
     4021 gcccgagata ctcggacagg cgccggcgat gcaggaagtg ttccgcgcca tcgggcggct
     4081 ctcacagtcg cacgcgacgg tgctgatcac cggcgaatcg ggcaccggca aggaactggt
     4141 ggcgcgcgcc ctgcaccgcc acagcccgcg ctcgggcagg cctttcatcg ccatcaacac
     4201 cgcggcgatc ccgcgcgagc tgctggagtc ggagctgttc ggacacgagc gcggggcctt
     4261 caccggcgcc caggcgcagc ggcgcgggcg cttcgagcag gcggagggcg gcaccttctt
     4321 cctcgacgaa atcggcgaca tgccgcagga gctgcagacg cggctgttgc gggtgctctc
     4381 cgacgggcac ttctaccgcg tcggcggcca ccagccgatc aaggccaacg tgcgcgtgat
     4441 cgcggcgacg catcaggacc tcgaggcgcg cgtcaagcag ggcctgttcc gcgacgacct
     4501 gttccaccgg ctcaacgtca tccggctgcg gctgccctcg ctgcgggagc ggcgcgagga
     4561 catcccgctg ctcgcgcgcc atttcctcgc ccgcagcgcg cgcgagctgg gggtcgaagc
     4621 gaaacggctg tccggggcgg cgctgaaata tctcgtcgcg caggactttc ccggcaacgt
     4681 gcgccagctc gagaacctgt gccactggct caccgtgatg gcgccctcgg tcaccatcga
     4741 agtgaaggac ctgccgcccg agctgcggac cgaggcggcg ccggcggcag cgagctggat
     4801 ggcggcgctg gagaaggagg tcgagacatt gctcaatcgc ggcgagaccg gcgtcatgga
     4861 cgagctcgcc cgccagttcg agaagacgct catcgtgaaa gcgctcgccc acaccggcgg
     4921 ccgccgcatc gaggcctcgc agctgctcgg cctcgggcgc aataccctta ctcgcaagat
     4981 ccaggaattg ggaatcgacg ccgaaaagaa gaatgggtga ctaagtgact aggtgattaa
     5041 gtgattgagc agcagcactc attaccgttc attcagttga ccactcaatc acctagtcac
     5101 ccaatcactc agtcactgat ttctgcggcc caatgattct caggtctgcg atgaccaggt
     5161 tgcgctggga gtaggagaat aacgtgtcac cgcccgcgag cttcttcaac gtgagcgtca
     5221 ccctgcggct gtccctccgg gcctcgctca gacggttgta aagctcgtcc acctcgagca
     5281 ccggcgcgcc gtccaccgcc tccagcaggt cggccttctc gatttcccgg gattccccga
     5341 tcgagcccgg ctcgacgtgg tgcaccatca ggccgcgcgc gccgatctcc agcgcgtcgc
     5401 ggatcgtgat cggcccgaac aggatgcctg aagcgtacac gccgcgggtc tccagcacgc
     5461 ggcgcatcgg caccttggcg ccgctgacgg cgacctcctg cccggcgcgc agcaccttga
     5521 gactcgaacc ggcgagccgg ccgcgaagcg cgtgcagcag ctgggtctcg ttctcgatcg
     5581 cgccctcctc gccgatcacc tgccggatga tgtcgccggg cagcagcttc aacgtcccgt
     5641 cgcccgcgta gctgcgcgcg accttgagct gcttgcggtt gtccacgtcc cggaaataga
     5701 cgagcttgag atcgggcggc gaagggtcca gcccttcctg cagcaactgc agcacccggc
     5761 acgcgtactt gatcgccacc gcgaaattgg tgttctgcgc gccgcgaatg ccggcggtgt
     5821 tgatgccgac gatgcggccg gtctcgaggc tgatcagtgg gccgccggaa ttgccctggt
     5881 tgatcggcgc gtcggtctgc aggagctccg tgcggtagcg ggcggtgacg ccggagatga
     5941 tgccgcgcgt gccggtgaac tgcagccgcc acggatggcc gaacgcgccc accggatgcc
     6001 cgaccggcgg cattccgccg cactcgagcc gcggctccac gatgccccgg gtgtcggcgc
     6061 gctcgggaa
//
LOCUS       DBNGWZ010000641         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2251632, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000641 DBNGWZ010000000
VERSION     DBNGWZ010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2251632"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16CX_20105"
     CDS             <1..315
                     /locus_tag="AC16CX_20105"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR3014845.1"
                     /translation="LVEARNNADSLAYAAEKALRELGDKVPGDDRKNIEKKIADLREA
                     IKGDDVARIRSLTEEVQQASYALSQQVYAGQQSAQSGGASRPSGNGKDEGEVVEGEFR
                     GT"
     gene            507..1082
                     /locus_tag="AC16CX_20110"
     CDS             507..1082
                     /locus_tag="AC16CX_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014846.1"
                     /translation="MFGKKPATPPPAQPKPQAPPAPQPPRPTGWPVQILTPDYVASGY
                     IAPVMDTPLVGFLNNPALPAIVLANAQLQALGPQAVIASATPAQVTIPKASLIAFIPR
                     DEAGMRSAAMQMPNRAERAIIYAGPYLLRAAFMLAGEMPLRNLFGTGGGSLVAVTEAE
                     VACQTPGAKFAPLKAPILVLNKAQVQLYHPA"
     gene            1086..1766
                     /locus_tag="AC16CX_20115"
     CDS             1086..1766
                     /locus_tag="AC16CX_20115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3014847.1"
                     /translation="MPFDPSKVRAVLFDLDGTLADTDDEYIRRAARWLGPLRLFFPWR
                     DPAPFLRWAVMTAESPLNTLMTIPDWMGIDDKLVAVTNWYSDLWPRQHVRRFVIMAGV
                     EALLERLSQRYPLALVTARPARSTTDFLEQFGLGWYFRAVASAQTAPHTKPYPDPVYW
                     CARALGVSVENCVMAGDTTADILTGKRAGAQTIGLLCGFGQRAELERVGADLVLEHTT
                     RLADVLLQ"
     gene            1913..2293
                     /locus_tag="AC16CX_20120"
     CDS             1913..2293
                     /locus_tag="AC16CX_20120"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR3014848.1"
                     /translation="MNENSHDRIRDVLQSLLDISPYMTATALVRVSGLTVMSFMPDGI
                     EQERVAAMSAVMLLLGERITGSLRSGALDKVYIKGEEGHIVLMAVGTEAVLTVMAQDH
                     APLGLLFVEMRRAAERLKRLVKEI"
     gene            complement(2334..>2833)
                     /locus_tag="AC16CX_20125"
     CDS             complement(2334..>2833)
                     /locus_tag="AC16CX_20125"
                     /inference="COORDINATES: protein motif:HMM:NF018816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR3014849.1"
                     /translation="AGALIYAVLYWVFKAFPMPLPFNQEIFIRPGVLVPLFMGLMFGP
                     LVGGATGVVGHLVGDLLAAAGFNLFWILGSGLMGFIPGIALYFIREYDTLRDYALAEL
                     FVALGVWGGSAVGALVGDALILRQGDFAAGMSKFVSEGLTGSINGLLLMPVLILIWKA
                     YRTRS"
BASE COUNT          536 a          917 c          915 g          465 t
ORIGIN      
        1 ctggtcgagg cgcgcaacaa cgccgacagc ctggcttacg ccgccgagaa ggcgctacgc
       61 gagttgggcg acaaggtccc gggcgacgac cggaagaaca tcgagaagaa gatcgccgac
      121 ctgcgcgaag ccatcaaggg cgacgacgtc gcgcgcattc gctcgctcac ggaggaggtc
      181 cagcaggctt cctacgcgct cagtcagcaa gtgtacgcag gccaacagtc cgcccaatcc
      241 ggtggggcca gccgcccttc gggtaacggc aaggatgaag gcgaagtcgt ggaaggcgag
      301 ttccgcggga cgtaaactgg ctgattgcag atggctgata gaagcaggtc acgcttcgag
      361 cgtgacctgc tttgtttaac caccagggcg tagcgcggcc tggccgcaat caggcacaac
      421 aacgaatcgg aacaacgcgg ctggtgaatc gggtacaatt gcgcgaactc gacagggaag
      481 cagagccgtt tcattcggag gattcgatgt tcggcaaaaa acccgccaca ccgcccccgg
      541 cgcagcctaa accgcaagcg ccgcccgcgc cccagccgcc gcgccccacg ggctggccgg
      601 tgcagatcct gacccctgat tacgtcgcga gcggctatat cgctccggtc atggacacgc
      661 cgctcgtcgg ctttctcaac aaccccgcgc tgcccgccat cgtgctcgcc aacgcgcagc
      721 tgcaagccct cggaccgcag gcggtgatcg cgagcgcgac gcccgcgcag gtcaccatcc
      781 ccaaagcgtc gctcatcgct ttcatcccgc gcgacgaggc cgggatgcgg tcggcggcga
      841 tgcagatgcc caaccgcgcc gagcgcgcca tcatctatgc cgggccgtat ctcctccgcg
      901 ccgcgttcat gctcgccggc gaaatgccgt tgcgcaatct cttcgggaca ggcggcggca
      961 gcctggtcgc cgtcaccgag gccgaggtgg cctgccagac ccccggggcg aagttcgctc
     1021 ccctgaaagc gcccatcctc gtgttgaaca aggcgcaggt tcaactctat catcctgcct
     1081 gacccgtgcc tttcgatcct tccaaagtcc gcgccgtcct cttcgatctc gacgggacac
     1141 tggccgacac cgacgacgaa tatatccggc gggcggcgcg gtggcttggc cctctacgcc
     1201 tcttcttccc ctggcgcgac ccggcgccct tcctgcgctg ggcggtgatg accgccgagt
     1261 cgccgctgaa cacgctcatg acgatccccg actggatggg gatagacgac aaactcgtcg
     1321 ccgtgacgaa ctggtactcc gacctgtggc cgcgacagca cgtgaggcgg ttcgtgatca
     1381 tggccggggt cgaggcgctg ttggagcgtc tgtcgcagcg gtacccgctc gcgctggtca
     1441 cggcccggcc cgcgcgcagc acgaccgatt ttctggagca gttcgggttg ggctggtatt
     1501 tccgcgcggt ggcctcggcc cagaccgcgc cgcacaccaa gccgtatccg gacccggtgt
     1561 attggtgcgc gcgggcgttg ggcgtgtccg tcgaaaactg cgtgatggcg ggcgacacaa
     1621 ccgcggacat cctcaccggg aagcgcgcgg gcgcgcagac gattgggctg ttgtgcggct
     1681 tcgggcagcg cgccgaattg gagcgcgttg gtgcggattt agtactggag catacgaccc
     1741 ggttggcgga tgtgctgtta caatgagaac aatgaggcag catccttcga cccccgctcc
     1801 gcttcgcttc gggggctata gtgcacggcg cgcactctga gcgaagtcga agagcgtgcc
     1861 gctcccctga gcgaagccga agggtcagga cgctaccgcc gccgaggccg ttgtgaacga
     1921 aaattcccac gaccgcatcc gagacgttct ccaatccctg ctggatatct cgccctacat
     1981 gacggcgacg gcgctcgtcc gcgtgagcgg gctgaccgtg atgtcgttca tgccggacgg
     2041 catcgagcag gagcgggtag cggcgatgtc cgccgtgatg ctgctgctgg gagagcgcat
     2101 caccggctca ctgcgcagcg gcgcgctgga caaggtgtat atcaaaggcg aagaggggca
     2161 tatcgtgctg atggcagtcg gtacggaagc ggtgctgacg gtgatggccc aggatcacgc
     2221 gccgctcggc ctgttgtttg tggagatgcg ccgcgcggcg gagaggttga agaggctggt
     2281 gaaggagata taggttcaga aacccggttt ctttgcgaaa ccgggtttct gttttagctt
     2341 ctagtgcggt acgccttcca gatcaggatg agcactggca tcagcagcaa accgttgatt
     2401 gatccggtca gtccttccga cacgaacttg ctcatcccgg cggcgaagtc cccctgccgc
     2461 aggatgagcg catcccccac gagcgcaccc accgcgctgc ccccccacac gcccaaggcc
     2521 acgaacaact ctgcgagcgc gtaatcgcgg agggtgtcat attcccggat gaaatagagc
     2581 gcaatgccgg ggatgaagcc catgagcccg ctgccgagaa tccaaaagag gttgaacccc
     2641 gccgccgcga gcaggtcgcc gacgaggtgg ccgacgactc cggttgcgcc tcccaccagc
     2701 gggccaaaca tcaaccccat gaagagcggc acaagcacgc cagggcggat gaagatttcc
     2761 tgattgaacg gcaagggcat ggggaacgct ttgaagaccc agtacagcac ggcgtagatg
     2821 agcgcgcccg ctg
//
LOCUS       DBNGWZ010000642         3816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_362314, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000642 DBNGWZ010000000
VERSION     DBNGWZ010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_362314"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /gene="dnaE"
                     /locus_tag="AC16CX_20130"
     CDS             complement(<1..1712)
                     /gene="dnaE"
                     /locus_tag="AC16CX_20130"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3014850.1"
                     /translation="MPAPSFVHLRLHSEYSIVDGIVRLDEAVEVAAADGMPALALTDL
                     GNVFGLVKFYQAARAAGVKPVIGCDLWLENEADRDKPYRMLLLCQSRAGYLRLSELLT
                     RAYRTHQHRGRPELKKSWFAEAGTEGLIALSGAHHGDVGQALVVDNPRQAQKLAREWH
                     EFFPGRFYLELQRLGKNAAVLGAAAIPVETYVQRALGLASAMKLPVVATHPVQFVKRD
                     DFRAHEARVCIAQGYTLSDQRRPRLYGPEQYFKTQTEMAQAFRDVPQALANSVEIAVR
                     CNLAIELGKTRLPAFRTPGNVSLDDYLRSRADEGLERRLPQLWPDAAERARHAPRYRE
                     RLEFEIRTIQQMGFSGYFLIVADFINWAKGNDVPVGPGRGSGAGSLVAYSLGITDLDP
                     LRYDLLFERFLNPERVSMPDFDIDFCQDGRDRVIEYVKGKYGADSVSQIVTFGTMAAR
                     AVVRDVGRVLDLPYTFCDQLAKLIPFQPGRHITLADARREEPQLKEREQKEEEVRELL
                     ALAEQLEGLTRNVGMHAGGVLIAPGKLTDFCPLYAADGSDSVVSQFDMKDVEAVGLVK
                     FDFLG"
     gene            complement(2091..2819)
                     /locus_tag="AC16CX_20135"
     CDS             complement(2091..2819)
                     /locus_tag="AC16CX_20135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR3014851.1"
                     /translation="MIPEISVRTEGAVYRVELNRPDCGNLVTMEMVAALTEAVQRVPG
                     DAKLVVLTGKGADFCKGRDYQAAPEAARGGRGPSALEMRERMAGPIISLYAALKELPV
                     PTLAIVQGAAYGFGCALAGGCDIVLAGEGTRFRLPEMGKGLPPTLAMAALDKVGAKGL
                     GYLVYSTAEIDARAALAMGLASAVCPDGELDAQAGKLMQAISSQPLDAVRAVKEYLKF
                     APLMEPRGRADFAASLIAGVLSSR"
     gene            2979..>3816
                     /locus_tag="AC16CX_20140"
     CDS             2979..>3816
                     /locus_tag="AC16CX_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3014852.1"
                     /translation="MKPSQSHFHDIRSLRYHVRTWGNPDAPRMFLLHGWMDVSASFQF
                     LVDAFSREWRVIAPDWRGFGLTEWAREGYWFPDYYADLEALLELHQPGEPARLVGHSM
                     GGVIASVYAGIRPDRVARLVSMEGLGLARHTADQAPVRYAQWLDQLMDPPGFRPYRAF
                     DAVAARLRKNNPRLSGERAAFLARHWAKQLDTGEVVLVSDPRHKIFNPYLFRIEEAIA
                     CWRRITAPVLLVSGKLSEMPARMKDTPEQLAERKGAFRDRREVELEDAGHMMHHDQPE
                     RLA"
BASE COUNT          639 a         1280 c         1228 g          669 t
ORIGIN      
        1 ccgaggaaat cgaacttgac cagcccgacc gcctcgacgt ccttcatgtc gaactgggac
       61 accacgctgt cggagccgtc cgcggcgtag agcgggcaga aatcggtgag cttgcccggc
      121 gcaatcagca ccccgcccgc gtgcatgccg acgttgcgcg tgagtccctc gagctgctcg
      181 gcgagcgcga gcagctcccg cacctcctcc tccttctgct cgcgctcctt caattgcggc
      241 tcctccctgc gcgcatcggc gagcgtgatg tgccggccgg gctggaacgg gatcagcttt
      301 gcgagctggt cgcagaaggt gtacggcagg tcgagcacgc gccccacgtc gcgcaccacc
      361 gcacgcgccg ccatggtgcc gaaggtgacg atttgcgaca cgctgtcggc gccgtacttg
      421 cccttcacgt actcgatcac gcggtcgcgc ccgtcctggc agaaatcgat gtcgaagtcg
      481 ggcatcgaga cgcgctcggg attgaggaag cgctcgaaca ggagatcgta acgcagggga
      541 tcgagatcgg tgatcccgag cgagtaggca accagcgatc ccgcccccga gccgcgcccg
      601 ggccccaccg gaacgtcgtt gcccttcgcc cagttgatga agtcggcgac gatcaggaaa
      661 tagcccgaga accccatctg ctggatggtc cggatttcga attcgagccg ctcccggtag
      721 cggggcgcgt gccgggcccg ctccgcggca tcgggccaga gctgcggcag gcggcgctcc
      781 agcccttcgt cggcccggct gcgcaggtaa tcgtcgaggc tgacgttccc cggcgtccgg
      841 aacgccggca ggcgagtctt gcccagctcg atcgcgaggt tgcagcgcac ggcgatctcg
      901 acgctgttgg cgagcgcctg cggcacgtcg cgaaacgctt gcgccatctc ggtctgcgtc
      961 ttgaaatact gctccgggcc gtagagacgc ggccggcgct ggtcggaaag cgtgtatccc
     1021 tgggcgatgc aaacgcgcgc ctcgtgggcg cggaagtcgt cgcgcttcac gaactgcacc
     1081 gggtgcgtcg ccaccaccgg cagcttcatc gcggaagcga gcccgagcgc gcgctgcacg
     1141 taggtttcga cggggatcgc cgccgcgccg agcacggcgg cgtttttccc cagccgctgc
     1201 agctcgaggt agaagcggcc ggggaagaac tcgtgccatt cgcgggccag cttctgcgcc
     1261 tgcctggggt tgtccaccac cagcgcctgc ccgacgtcgc cgtggtgcgc gccggaaagc
     1321 gcgatcaggc cttcggttcc ggcttccgcg aaccagctct ttttcaactc gggccgcccc
     1381 cggtgctggt gggtccggta ggcgcgggtg agcagctcgg agagccgcag gtagccagcc
     1441 cgcgactggc aaagcagcaa catgcggtag ggcttgtcgc ggtcggcctc gttctcgagc
     1501 cagagatcgc agccgatgac gggcttgaca cccgctgcac gggccgcctg gtaaaacttg
     1561 accaggccga agacgttgcc gaggtcggtc agcgccaacg ccggcatgcc gtcggccgcc
     1621 gccacctcga ccgcttcgtc gaggcggacg attccatcca cgatcgaata ttcactgtgc
     1681 aatcgcagat ggacgaacga tggcgcgggc atagggagcc tatctcacgg cccatcgcgt
     1741 gtgcgacgga agcgagttgg gatgcagttc taggagggcg cgacgacgca tagactctat
     1801 gctaggagcg atccgacaac gaaatgcgcc caaatcgctc cgccccttcg ggttgcgacc
     1861 agaacgggcg cctgctttgt cgcgcattca tcccgttctg atcgcaacgc atcacgtgag
     1921 gggccgtgag atagactcct tattttacgc cgtgctaatt ccttttttaa tggattttcg
     1981 gtggtcgacg gaaaagacac gagcccgcgc cataaaggat gaaggatgaa gtaaacgact
     2041 gttagacagc gctctttccg ccttctgcct tctgccttct gccttctgcc tcagcgggag
     2101 gacagcactc ccgcgatgag actcgccgcg aagtccgccc ggccacgtgg ctccattaac
     2161 ggtgcgaact tgaggtactc cttcaccgcc ctcacggcgt cgagcggctg ggacgagatg
     2221 gcctgcatga gcttgcctgc ctgcgcatcc agttcgccgt cgggacagac cgcgctcgcg
     2281 agccccatgg ccagcgccgc ccgggcatcg atttccgcgg tcgaatagac gaggtagcca
     2341 agacctttcg ctccgacctt gtcgagggcc gccatcgcga gcgtgggcgg cagccccttt
     2401 cccatctccg ggagcctgaa gcgggtgcct tcaccggcca gcacgatgtc acaaccaccc
     2461 gccagcgcgc agccgaagcc gtaggcggcg ccctgcacga tcgcaagcgt cggcaccggc
     2521 agctccttga gcgctgcata gaggctgatg atgggacccg ccatgcgttc acgcatctcg
     2581 agcgcgcttg gcccccgccc gccgcgggca gcctccggcg ccgcctggta atcccgcccc
     2641 ttgcagaaat ccgctccctt tccggtgagc accaccagct tggcgtcgcc cggcacgcgc
     2701 tggaccgcct cggtcagcgc cgcgaccatc tccatagtga cgagattgcc gcagtcgggg
     2761 cggttgagct ccacgcggta aacggcgcct tcggtgcgga cggaaatttc cgggatcata
     2821 aatacgtggg tcgtggatcg tgggtcgaga ctcgatgcag atcgccggat tctaacgctg
     2881 aatcgcggat cacgaatctt tctctagcgc ttccctgttc atcatttacc attcactatt
     2941 caccattcac tattcaccat tcactattca ccgcctttat gaagccgagt caatcgcatt
     3001 tccatgacat ccgcagcctc cgctaccacg tgcgcacgtg gggcaatccg gatgcgccgc
     3061 ggatgtttct gctgcacggg tggatggacg tgtcggcgtc gttccagttc ctggtggatg
     3121 cgttctcgcg cgaatggcgc gtcatcgcgc cggactggcg cggcttcggg ctcaccgaat
     3181 gggcgcgcga aggctactgg tttcccgatt actatgccga tctggaagcg ctgctcgaac
     3241 ttcatcagcc gggcgagccg gcccgtctcg tcggccacag catgggcggc gtcatcgctt
     3301 ccgtctatgc cggcatccgc ccggatcgcg ttgcccgact ggtgtcgatg gaagggttgg
     3361 gactggcgcg ccatactgcc gaccaggcgc ccgtgcgtta cgcgcagtgg ctcgaccagc
     3421 tcatggaccc gccgggattc aggccgtacc gcgcgttcga cgccgtcgcc gctcgcctcc
     3481 ggaagaacaa tccgcggctg tcgggcgagc gggcggcgtt tctcgcccgg cactgggcga
     3541 agcagctcga cacgggcgag gtcgtgctgg tgagcgaccc gcgccacaaa atcttcaacc
     3601 cgtacctgtt ccggatcgag gaggcgattg cgtgctggcg gcggatcacg gcgccggtgc
     3661 tgctcgtgtc aggcaaactg tcggagatgc cggcacgcat gaaggacacg ccggagcagc
     3721 tcgccgaacg caagggcgcg ttccgcgacc gccgcgaggt cgagctcgag gatgcggggc
     3781 acatgatgca ccacgaccag cccgagcggc tggcgc
//
LOCUS       DBNGWZ010000643         5349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_236643, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000643 DBNGWZ010000000
VERSION     DBNGWZ010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_236643"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            640..1383
                     /locus_tag="AC16CX_20145"
     CDS             640..1383
                     /locus_tag="AC16CX_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014853.1"
                     /translation="MDRALWISWYDLPDNGRDAYLKWAHDTYIPKLIKHPGFLWGAHF
                     KSESEPIQSGVPRGAPGRLRHTDDPAVPTGSVYILIFGAENAHAFGNPTPRKLHAALS
                     KEDQKMLAMRIGERVNIFTDENQIGGPEAGKRDPKAALSPCIQLGSFNAGSADEDEIL
                     AWYTQWRLPSMNKLPGCVGIRKLVSVSGWAKHGVLYEFVSLDARNKHFPDHEKANPEM
                     DAWTDRLVRQLVHAPGSPCVAQRLFSVVK"
     gene            1443..1844
                     /locus_tag="AC16CX_20150"
     CDS             1443..1844
                     /locus_tag="AC16CX_20150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014854.1"
                     /translation="MAMSEFERKRWDRGMEILREMGREQTMMNQKALYPDLYNLSVGY
                     LFGEIWSRPHLSIRDRELITLAANIALARPAGTHSHYRSAQHIGITKEQIMELIIHVG
                     HYAGWPAIAHAIIQFEQVLKEDAEKAGAQKK"
     gene            1986..2939
                     /locus_tag="AC16CX_20155"
     CDS             1986..2939
                     /locus_tag="AC16CX_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014855.1"
                     /translation="MIRIFMMAMMLALAVPAWAQGYPAKPVRFLVSFSAGSGSDTIAR
                     IVAGGLGQAFGQQVIVENRAGAAGNIGAELAAKSPPDGYTLFLVNMGHAANATLYEKL
                     PYNLVRDFAPVTQLASSPSIVVVHPSLPVKSIGELVRLAKAKPGAIHFGSGGIGTPTF
                     VAGELFKRQAGVDLLHVPYKSGGEAIIGVVTGEVPLYFAPLATALPHVQQGKLRALAV
                     TTPRRLPLLPDYPTVAETGYPGYQAGNWYGLLVPARTPKEAITAIRAAAVTALNYPAV
                     AKRMTDLGYVIIGDQPEEFAAHIRSEIASLAKVLKGLRAGG"
     gene            3264..3566
                     /locus_tag="AC16CX_20160"
     CDS             3264..3566
                     /locus_tag="AC16CX_20160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3014856.1"
                     /translation="MVLEVAILHIKPEEIKNFEAVFPKVEPIFRSVKGYISHELQRCV
                     ETKGRYHFLIRWETIESHTVNFRQSPKFQEFRSLVGGFFDKPPVAEHFERVTESHI"
     gene            complement(3682..4116)
                     /locus_tag="AC16CX_20165"
     CDS             complement(3682..4116)
                     /locus_tag="AC16CX_20165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3014857.1"
                     /translation="MKKAKSRDYDDRFGRGMRVLKRMGRENLMLNQKALYPDMYEMSV
                     GHLFGDVWARPGLSLRDRQLVTLAANIALARPTGNHSHYLSALHLGITKREICEVMIQ
                     VGHYAGWPALSNAVRQFTDILEEQRKMEQAKKLGRPFKSRLL"
     gene            4224..5216
                     /locus_tag="AC16CX_20170"
     CDS             4224..5216
                     /locus_tag="AC16CX_20170"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014858.1"
                     /translation="MKPIVPLLFASCIAAAPQALAQKAQTAGAAGYPSKSIRLVVPSA
                     PGGGTDIIARMIAQGLSDAWGQTVVVDNRGGAGGIAGVSIVAKQSAPDGYSMLLGSVG
                     HLSFAPAVRPNLGFDPLKDLTPISLAAVQPFVVAASNSLPVKSIKELVALAKAKPGSI
                     SYGSGGSGTASHLGTELLMINGGFKMLHVPYKGSNPAITALMGGELQVALAGLATVLP
                     HAKSGRLKALAVTGAKRAKIAPEVPTVGESGVPGYVFDVWYGLVFPGGTPTAIVKKTN
                     AEVVKQLQSPEVSSRFAGAGVEPQTNTPEQFADLIRREVVTWTKVVKAANIKVE"
     gene            5216..>5349
                     /locus_tag="AC16CX_20175"
     CDS             5216..>5349
                     /locus_tag="AC16CX_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HIR3014859.1"
                     /translation="MAAQLEGKVALVTGAVRRNGRAIAHALAREGAGIVINTRRSRDE
                     A"
BASE COUNT         1052 a         1615 c         1694 g          988 t
ORIGIN      
        1 acgggtaacc gctacggcga tcctccgggg cacgcctgca tcgcggtgat cggcggcgcc
       61 gagcgcacga tcaccctgga caccggaagc aaagaccgca tcgccaacat gtacgcgttc
      121 gccaccgcgg cgctgaagct actggcggag agcctcgaaa gccggtgact cgtgactggt
      181 gtttgagtcc attcactatt cactagagca tgtctcaaaa atacccagca accccggaaa
      241 ccgccgatga gcgccgatac acgcaaatat atcaagagca agccaaacga ttggttctgc
      301 tttacgtcgg cgttaatcgg cgattatccg cggatcacct gtttaggatt ctcgaggcaa
      361 gtcacggatt tcaaagacaa gaaaagcagg cttgcggtat atggctgcgg ataaacgccg
      421 aagagcatcg ctgctgacga cttcgccgga atttgaacta tccgtgttca tctgcgttca
      481 tctgcggttt catcaggcgt ttcgagatac cttctactca cctctcacgt tgtcccggct
      541 tggtcgaccc acggactctg aagaccatat tgtgggaaag cgggtgcgaa aggacgcggc
      601 ttcctgcaat atcccgatct cgattaggga ggacatatag tggaccgagc gctttggatc
      661 tcctggtacg accttcccga taacgggcgc gacgcctacc tgaaatgggc acacgacact
      721 tacatcccga agctgatcaa gcatccgggc ttcctgtggg gcgcgcactt caaatcggaa
      781 tcggaaccga tccagtcggg cgtgccacgc ggcgcgccgg ggcggcttcg ccacaccgat
      841 gatccggcgg ttccgaccgg cagcgtctat atcctgatct tcggcgcgga aaacgcgcac
      901 gccttcggca acccaacccc gcgcaagctg cacgcggcgc tatcgaagga ggaccaaaag
      961 atgctcgcga tgcggatcgg ggaacgcgtg aacatcttca ccgatgagaa ccagatcggc
     1021 ggaccagaag cgggcaagcg cgacccgaag gcggcgctga gcccgtgcat ccagctcggc
     1081 agcttcaacg cgggctccgc ggacgaggac gagatcctgg cgtggtatac gcagtggcgc
     1141 ctgccctcga tgaacaagtt gcccggctgt gtcgggatcc gcaagctggt ttcggtttcc
     1201 ggctgggcga agcacggcgt cctctatgag ttcgtgtcgc tggatgcgcg caacaagcac
     1261 ttccccgacc acgagaaagc gaatcctgaa atggacgcct ggaccgaccg gctggtgcgc
     1321 cagcttgtgc acgcgcccgg ttctccgtgc gtcgcgcagc gcctgttctc ggtcgtgaag
     1381 tgatcgcgtg atccgcagcg gcacgcgttc gaagcatcaa ttgactgaaa agggacaaaa
     1441 ccatggcgat gagcgaattc gagcggaagc ggtgggacag ggggatggag atcctgaggg
     1501 agatgggccg cgagcagact atgatgaacc agaaggcgct ctaccccgac ctctacaacc
     1561 tgtcggtcgg ctacctgttc ggcgagatct ggtcgcggcc gcatctttcc atccgcgatc
     1621 gggagctgat cacgctcgcg gcgaacatcg cgctggcgcg gccggccggc acgcactcgc
     1681 attaccgcag cgcccagcac atcggcatca ccaaggagca gatcatggaa ttgatcatcc
     1741 acgtggggca ctacgccggc tggccggcga tcgcccacgc gatcatccag ttcgagcagg
     1801 ttctgaagga agacgctgaa aaggccgggg cgcagaaaaa gtagggcgcg ggcggcggcg
     1861 gaagggttcg ggagaaccgg tgatgaggtg acgaggtgac gaggtgacga ggtgacgaag
     1921 aaaacggtat ggctcgtcac agcgtcactg catcactgcg tcaccttttt atcgcggagg
     1981 gagtggtgat cagaatattc atgatggcta tgatgctggc gctcgccgtt ccggcatggg
     2041 cgcaaggtta tcccgcgaag ccggtccgtt tcctggtgtc gttctcggcc ggcagcggct
     2101 ccgataccat cgcccgcatc gtcgccggcg ggctcggcca ggcgttcggg cagcaggtca
     2161 tcgtggaaaa ccgcgccggg gccgcgggca acattggcgc cgagctcgcc gcgaaatcgc
     2221 cgcccgacgg ctacacgctg ttcctggtca acatgggtca cgccgcgaac gcgaccctct
     2281 acgagaagct gccgtacaac ctcgtgcgcg atttcgcgcc ggtcacccag ctcgcctcct
     2341 cgccctcgat cgtcgtcgtg cacccgtcgt taccggtgaa gtcgatcggc gagctcgtca
     2401 ggctggcgaa ggcgaagccc ggcgccatcc atttcggatc cggcggcatc ggcacgccca
     2461 cgttcgtcgc cggggaattg ttcaagcggc aggccggcgt ggacctgctg cacgtgccgt
     2521 acaagagcgg cggcgaggcg atcatcgggg ttgtgaccgg cgaagtcccg ctgtacttcg
     2581 cgccgctcgc gaccgcgttg ccgcacgttc agcagggcaa gctgcgcgcg ctcgcggtga
     2641 cgacgcccag gcgcctgccg ctgttgcccg actacccgac ggtggccgag accggctacc
     2701 ccggctacca ggccggcaac tggtacggcc tgctggtgcc ggcgaggacg ccgaaggaag
     2761 cgattacggc gatccgcgcc gcggcggtca cggcgctgaa ctatccagcc gtggcgaagc
     2821 gcatgacgga cctgggctac gtcatcatcg gcgaccagcc ggaggaattc gcggcgcaca
     2881 tccgctcgga gatcgcgagc ctggcgaagg tcctgaaagg cctgcgcgcg ggcggttgaa
     2941 gccgatcagg aaccgccgat gaacgccgat acacgcagac aagttgatag caatcccaat
     3001 gattggatct gctttacatc ggcgttaatc aacggattat ctgtggttca atcctgtttt
     3061 gattattgag acaaatcact ggcaccattc aaaggcaaaa gaggtggacg aaatctagcg
     3121 ggttcggatg aacgcgggtg caagcccctg ctgatgccat cttcgggatt cgaatatctg
     3181 tgttcatctg cgttcatctg cggtttcatt cggatcttct cgacagcttc tagacgtcat
     3241 tattaactga taggagaaca atcatggttc tggaagtcgc catcctgcac atcaagcctg
     3301 aggaaataaa gaatttcgag gccgtttttc ccaaggtcga gcccatcttc cgcagcgtca
     3361 agggctacat ctcgcacgag ctgcagcgct gcgtggagac taagggccgc taccacttcc
     3421 tgatccgctg ggagaccatc gaatcccata cggtcaactt ccggcagtcg ccgaagtttc
     3481 aggaattccg cagcctggtc ggcgggttct tcgacaagcc gccggtcgcc gagcatttcg
     3541 agcgggtcac cgagagtcac atttgactct caggtccaag ttacaagtaa caagctacaa
     3601 gttgcaagcc ttgaaggttg tatcttgaac cttgaaactt ggaacttgaa ccttgaaact
     3661 tggaacttgg aacttgagac tttatagaag tctggacttg aacggcctgc ccagcttctt
     3721 cgcctgttcc atcttcctct gctcttcgag tatgtcggtg aactggcgca cggcgttcga
     3781 cagcgccggc cagccggcat agtgcccgac ttgtatcatt acctcgcaga tttcccgctt
     3841 ggtgatgccg aggtgcagcg cgctcaggta atgcgaatgg ttgccggtcg ggcgcgccag
     3901 ggcgatgttc gccgcgagcg tcacgagctg ccggtcgcgc aggctcaacc cggggcgtgc
     3961 ccagacgtcg ccgaacaggt gcccgaccga catctcgtac atgtcgggat agagcgcctt
     4021 ctggttgagc atcaaatttt cccggcccat cctcttcagc acgcgcatgc cgcgcccgaa
     4081 acggtcgtcg tagtcccgtg atttcgcttt tttcatggtg tggggtctca cagtatggag
     4141 tgtgataaat tttgcgtgat catgtcatcc cggcccgtgc cgggcaagcg cccgctgccg
     4201 gcgtgcatcg aagagggctc acaatgaaac cgatcgtacc tttgctgttc gcttcgtgca
     4261 tcgcggctgc gccgcaggcg ctcgcgcaga aggcgcagac cgcgggcgcc gccggctacc
     4321 cgagcaagtc gatccggctg gtggtaccga gcgcgccggg tggcggcacc gacattatcg
     4381 cgcgcatgat cgcacaggga ctcagcgacg cctggggcca gaccgtggtg gtggacaacc
     4441 gcggtggcgc cggcggaatt gcgggtgttt ccatcgttgc caagcagtcc gcgcctgacg
     4501 gctattccat gctgcttggc agcgtgggcc atctctcgtt cgcgcccgcg gtccgtccca
     4561 atctgggatt cgatccgctg aaggatctca cgcccatatc gcttgctgcg gtccagccgt
     4621 ttgtcgtagc ggcgtcgaac tcgttgccgg tcaagtcgat caaggagctg gtcgcgctgg
     4681 cgaaagcgaa gcccggctcg atcagctacg ggtccggcgg tagcggcacc gcctcgcacc
     4741 tcggcaccga gctgttgatg atcaacggcg gcttcaagat gctgcatgtc ccgtacaagg
     4801 gaagcaaccc ggccattacc gcgctgatgg gcggagaact ccaggtcgcg ttggcggggc
     4861 tcgcaacagt gctcccgcac gccaagagcg gcaggttgaa agcgctcgcc gtgactggtg
     4921 cgaaacgcgc gaagatcgcg cccgaggtgc caacggtcgg agagtccggc gtgccgggct
     4981 acgtctttga cgtgtggtac gggctggtct ttccgggcgg gacgccgacc gcgatcgtga
     5041 aaaagacgaa cgcggaggtc gtgaagcagc tgcaatcgcc ggaagtcagc agccggttcg
     5101 cgggcgccgg cgtcgagccg cagaccaaca cgccggagca attcgcggac ctgatccggc
     5161 gcgaggtcgt gacctggacc aaggtggtga aggccgcgaa tatcaaggtt gagtaatggc
     5221 tgcacaactc gagggcaagg ttgcgctggt gaccggcgcg gtgcggcgca acgggcgcgc
     5281 catcgcacac gcgctcgcgc gcgagggcgc ggggatcgtc atcaacacgc gccgctcgcg
     5341 cgacgaggc
//
LOCUS       DBNGWZ010000644         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1118968, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000644 DBNGWZ010000000
VERSION     DBNGWZ010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1118968"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC16CX_20180"
     CDS             complement(<1..341)
                     /locus_tag="AC16CX_20180"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363073.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3014860.1"
                     /translation="MNARVELANAIRALAMDAVQQANSGHPGMPMGMAEIAEVLWNHH
                     LRHNPANPAWANRDRFVLSNGHGSMLLYSVLHLTGYDLPLEELKRFRQLHSKTPGHPE
                     YGCAPGVETTTG"
     gene            complement(431..619)
                     /locus_tag="AC16CX_20185"
                     /pseudo
     CDS             complement(431..619)
                     /locus_tag="AC16CX_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(713..829)
                     /locus_tag="AC16CX_20190"
                     /pseudo
     CDS             complement(713..829)
                     /locus_tag="AC16CX_20190"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
     gene            complement(941..1693)
                     /locus_tag="AC16CX_20195"
                     /pseudo
     CDS             complement(941..1693)
                     /locus_tag="AC16CX_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743627.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
     gene            complement(1836..>2023)
                     /locus_tag="AC16CX_20200"
     CDS             complement(1836..>2023)
                     /locus_tag="AC16CX_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3014861.1"
                     /translation="PYVTTKPKGTGLGLAIVKKIVEEHGGTAAIANIAPRGARVTIHL
                     PAAAQDVSRRSAARAQV"
BASE COUNT          312 a          661 c          687 g          363 t
ORIGIN      
        1 ccggtagtgg tctcgacgcc gggcgcgcaa ccgtattccg ggtggccggg cgtcttcgag
       61 tgcagctgcc ggaagcgctt gagctcttcg agcggcagat cgtagccggt caggtgcagc
      121 acggaataga gcagcatcga gccgtggccg ttggatagca cgaagcggtc gcggtttgcc
      181 caggcggggt tggcgggatt gtgccgcagg tggtggttcc acagcacctc cgcgatctcc
      241 gccatgccca tcggcatacc gggatgcccg gaattcgcct gctgtacggc gtccatcgcc
      301 agcgcgcgga tcgcgttggc cagctcgaca cgggcgttca ttcggatctt ccttgcgagg
      361 aggggcgaga agtgcgccaa ttataaactg gcgaggcgcg tgacggagcg cgcgcgcctg
      421 aagaaaacgg ctaatcgccg ttttcggttt tccgggcgag gcggatgccg agctgcttca
      481 acttgcggta gaggtgggtg cgctcgagcc cgacgctttc cgccacgcgg ctgatattgc
      541 cgccctcgcg cgcgatgtgg tactcgaaat aggtgcgctc gaaggcgtcg cgcgcgtcgc
      601 gcagcggcat gtcgaaagcc aggctgtgcg cgacgggcac caccgcggcc ggctggggca
      661 gggcgcggct gacctcctcc agcgggatct gctcggaagg gcaggtcagc gcaagcgtgc
      721 gcaccacgct ctcgagctgc acgagattac ccggccagtc gtagattctc agcgcgttga
      781 gggccccggt ggtgaactga cgcggcggcg cttccttcgc ttccaccatg cgcgagagcg
      841 tcaggttggc gatgtcgggc acgtcctcgc ggtgctcgcg caggctcgga acccggatgg
      901 cggctgccgc cagcacgccg tagagccggc tgtcgaaggc gccctgcgcc acccgctccg
      961 cgaggtcgcg gctggcgccg ctcaccagcc gcacgttgta gcgctcgagt ttggaggcgg
     1021 cgagcagcag ccctttctgc tcgacccggg acaggtcaca aacctcgtgc aggaacagcg
     1081 ttccctcgcg cgcctggttg aggagatcaa gcggcgcctc ggcgagcgcg gaatggtttt
     1141 ccggcgcgac ccaaggcgtg ttgcgctggt gcaggaaccg cgccgccagc tcgatgccgc
     1201 agcccggctc ccccgtgagc agcacgggcg tgcgcaggct catcacccgc tcgaggcgct
     1261 gcttcagctc gatcaccagc agcgagcggc ccaggctcgc cagcgacagc gccgggtgcg
     1321 cctgttcgcc gccgtgcttg agcgcgcgcc cgacggtggc gagcagcttc tgcagcgcga
     1381 tcggtttctc gaggaaatcg taggcgccga tgcgggtcgc ctggaccgcg gtgtcgatgg
     1441 tgccgtgccc cgacatcatc accaccggca tggtgagcag tcccgccgcc gcccattcct
     1501 tgagcagcgt gatgccgtcg gtgtccggca tccagatatc gagcagcacc aggtcggggc
     1561 gcccctggtt acggagcgag cgcgccgcgc tcgcgttttc cgcgagccgc acctgatagc
     1621 cttcatcgct caggatttcg gacagcagct cgcgaatgcc gatttcgtcg tctacgacca
     1681 ggatttgttg cataaccaat tttcagcagg ctgttgaaaa tatcccgtgg gttttctcag
     1741 gatacagcca ttgcttccat ttgactctca acagcctctg acttggggga tacaaaaggg
     1801 ggcatcgccc cctttttcaa taccccgtta gtttattaca cctgcgcccg cgcggccgac
     1861 cggcgcgaga cgtcctgcgc cgccgccggc aggtggatgg tgacgcgcgc gccgcgcggc
     1921 gcgatattcg cgatggctgc cgtgccgccg tgctcctcga cgatcttctt gacgatcgcc
     1981 agccccaacc cggtgccctt gggcttggtg gtgacataag gct
//
LOCUS       DBNGWZ010000645         3119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1623146, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000645 DBNGWZ010000000
VERSION     DBNGWZ010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1623146"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..237
                     /gene="lptM"
                     /locus_tag="AC16CX_20205"
                     /gene_synonym="yifL"
     CDS             82..237
                     /gene="lptM"
                     /locus_tag="AC16CX_20205"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR3014862.1"
                     /translation="MRALVPLIVLSLLLAACGYKGPLYLPKPKPEAQKPATPPDSQET
                     EKKPAAQ"
     gene            234..1475
                     /gene="lysA"
                     /locus_tag="AC16CX_20210"
     CDS             234..1475
                     /gene="lysA"
                     /locus_tag="AC16CX_20210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3014863.1"
                     /translation="MSHFEYKNNELHAESVPLARIAERYGTPCYVYSRAALTEAYRAF
                     DAAFAGRDHLVCYAVKANSTLAVLNVLARLGSGFDIVSGGELARVLAAGGDPNRVVFS
                     GVGKTAGEMRQALEAGILCFNVESEGELERLEAVAAQTGKTAAVSLRVNPDVDPRTHP
                     YIATGLKQNKFGIAYEDALGLYRRARTMRHLAVTGIGCHIGSQLTEVAPFVAALERLL
                     ELADRLHAEGFSLTHVDIGGGLGIRYRDESPPAIADYAHALTGRLRSRALKLLLEPGR
                     ALAGHAGLLLTRVEYLKHGRERNFAVVDAAMNDLLRPALYDAWHEVLPVAAAGGGKTY
                     EIVGPVCESGDFLARSRSLALAEGSLLAIMAAGAYGMSMSSNYNSRPRAAEVMVDGGE
                     AHLIRERETVSELMAREIMLP"
     gene            complement(1557..2315)
                     /locus_tag="AC16CX_20215"
     CDS             complement(1557..2315)
                     /locus_tag="AC16CX_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3014864.1"
                     /translation="MSRLPVTESDLHAYIDGVLPAERASEVEAYLAAHPEDAARVAAY
                     CEQTAALHRAFDPVLDEPLPRKLEVPRPLWIRPVLRYAAVVAWVAFGGVIGWQLHAYT
                     STSIRMEPAAWARRAAIAHAVYSPEVRHPVEVGADQEAHLVAWLSKRLGAQITAPHLG
                     GLGYTLVGGRLLPGERGPVAQFMYQDGRGQRLTLYVRVNPDDSRETAFRFAQERGVGV
                     FYWIDRKLGYALSGEVDKSELLRVATAVHRQLNP"
     gene            complement(2312..2824)
                     /locus_tag="AC16CX_20220"
     CDS             complement(2312..2824)
                     /locus_tag="AC16CX_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3014865.1"
                     /translation="MAEYDQLIEQQIPRLRRYARALTGDRAPADDLVQDTLERALSRF
                     HLWRRGSDLRAWLFTIMHNIYVNQTRARLRHLHESLESEPAAETVHAREPDWLELRDL
                     DGALARLPHEQRAVLLMVGLEQFTYEEAARMLDIPIGTVMSRLSRGRERLRAILGGEA
                     PSITPFKVVK"
     gene            complement(2827..>3119)
                     /locus_tag="AC16CX_20225"
     CDS             complement(2827..>3119)
                     /locus_tag="AC16CX_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COG4315 family predicted lipoprotein"
                     /protein_id="HIR3014866.1"
                     /translation="PAGMTLYTFDKDPAGKSVCNDKCAANWPPLKASAGARPGGEFGI
                     VTRDDGSKQWAYKGMPLYLWIKDLKPGDMSGDGVNNVWHVVKEDLHPVHSGD"
BASE COUNT          497 a         1084 c         1030 g          508 t
ORIGIN      
        1 ccgttcactg cttaccgttt accgcttact gctcaccgca tgctgtttac tgcttaccgc
       61 ttaccgctta ccatctgatc catgcgcgcc ctggttccgc tgatcgttct ttccctgctg
      121 ctcgcggcgt gcggctacaa aggcccgctg tacctgccga agccgaagcc cgaagcacaa
      181 aagcccgcga cgccgccgga ctcgcaagag accgagaaga aaccggccgc tcagtgagtc
      241 acttcgaata caagaacaac gagctccacg ccgagtcggt gccgctcgcc cggatcgcgg
      301 agcgctacgg cacgccgtgc tacgtttatt cgcgcgccgc gctgacagag gcctatcgcg
      361 cgttcgacgc cgcgttcgcc ggccgcgatc acctcgtctg ctacgcggtg aaggcgaatt
      421 ccacgctcgc cgtgctcaat gtgctcgccc gcctcggcag cggcttcgac atcgtgtccg
      481 gcggcgagct cgcgcgggtg cttgccgccg gcggcgatcc gaaccgggtg gtgttctccg
      541 gcgtcggcaa gacggccggg gagatgcgcc aggcgctgga ggcgggcatc ctgtgcttca
      601 acgtcgagtc ggaaggtgag ctcgagcgcc tggaggccgt ggcggcgcaa accggcaaga
      661 ccgcggcggt gagcctgcgc gtcaaccccg acgtggatcc ccgcacccat ccgtacatcg
      721 cgaccgggct gaagcagaac aagttcggca ttgcctacga ggacgcgctc gggctgtacc
      781 ggagagcgcg cacgatgcgc cacctcgccg tcaccgggat cggctgtcat atcggctcgc
      841 aactcacgga ggtggcgcca ttcgtggccg cgctcgagcg cctgctggaa cttgccgacc
      901 ggctgcacgc cgagggcttc tccctcacgc atgtggacat cggcggcggc cttggaatcc
      961 gttatcggga cgaatcgccg ccggcgatcg cggactatgc ccatgcactg acgggccgcc
     1021 tgcgcagccg cgcgttgaag ctgctgctcg agcccgggcg ggcgctggcc ggacacgccg
     1081 ggctgctgct cacccgcgtc gaatacctca agcacggccg cgaacggaac ttcgcagtgg
     1141 tggacgcggc gatgaacgac ctcctgcggc cggcgctcta cgacgcctgg cacgaagtgc
     1201 tgccggtcgc cgccgccggc gggggcaaga cctacgagat cgtcggcccg gtgtgcgaga
     1261 gcggcgattt cctcgcccgc tcgcgctcgc tcgcgctcgc cgaaggcagc ctcctcgcga
     1321 tcatggcggc cggcgcctac ggcatgagca tgagctccaa ctacaactcc cgcccgcgcg
     1381 ccgcggaggt gatggtggac ggcggcgagg cgcatctcat ccgcgagcgc gaaacggtgt
     1441 ccgagctgat ggcgcgggag ataatgcttc cgtgagggcg tgagagcgtg agggcggaag
     1501 atggaaaaca ctccatctca cgacctcccg atctcccgat ctcccggtac tgcggctcag
     1561 gggttgagct ggcggtggac ggccgtcgcc acccgcagca gctcggactt atccacctct
     1621 cccgagagcg cgtaaccgag cttgcggtcg atccagtaga acacgccgac gccgcgctcc
     1681 tgcgcgaagc ggaacgcggt ttcgcggctg tcgtccgggt tgacgcggac gtagagcgtc
     1741 agccgctggc ctctgccgtc ctggtacatg aactgcgcga ccggtccgcg ctcgccgggc
     1801 agcagccgac cgccgaccag cgtgtatccc aggccgccaa gatgcggcgc cgtgatctgc
     1861 gcgcccaggc gcttcgagag ccacgccacg agatgcgctt cctggtccgc ccccacctcg
     1921 accggatggc gcacctcggg gctgtagacc gcatgcgcga tcgccgcccg acgcgcccat
     1981 gcggcgggct ccatgcggat cgacgtgctg gtgtaggcgt ggagctgcca gccgatcacc
     2041 ccgccgaacg cgacccacgc caccaccgcg gcatagcgca acaccgggcg tatccagagc
     2101 gggcgcggga cctcgagctt gcgcggcaac ggctcgtcga gcaccgggtc gaatgcgcga
     2161 tgcagcgcgg cggtctgctc gcagtaggcc gcgacccgcg ccgcgtcttc cggatgggcc
     2221 gcaaggtacg cttccacttc ggaggcacgc tcggccggga gcacgccgtc gatataagca
     2281 tgcagatccg attcggttac aggcagcctg ctcatttcac gaccttgaac ggggttatgc
     2341 tgggagcttc gccgcccagg atcgcgcgca gccgctcgcg cccgcgcgac aggcgcgaca
     2401 tcacggtgcc gatcgggata tcgagcatac gtgcggcttc ctcataggtg aattgctcca
     2461 ggccgaccat cagcaacacc gcgcgctgct cgtggggtag ccgcgccagc gcgccatcga
     2521 ggtcgcgcag ctcgagccaa tccggctcgc gggcgtgcac tgtttcggct gcaggctcgc
     2581 tctcgagcga ctcgtgaaga tgccgcagcc gggcgcgggt ctggttgacg taaatgttgt
     2641 gcatgatggt aaagagccac gccctgaggt cgctgccacg gcgccataga tggaagcggg
     2701 aaagcgcccg ttccagggtg tcctgcacga gatcgtcggc cggggcgcgg tccccggtca
     2761 gggcgcgcgc gtagcgccgc aggcgcggta tctgctgctc aatcaactga tcgtattcgg
     2821 ccacggctag tcgccggaat gcaccgggtg caagtcttcc ttgaccacgt gccagacgtt
     2881 gttgacaccg tcgccgctca tgtccccggg tttcaagtcc ttgatccaca ggtaaagcgg
     2941 catgcccttg taagcccact gcttcgaccc gtcgtcgcgc gtgacgatgc cgaattcgcc
     3001 gccgggcctc gcccccgcgc tggccttcag cggcggccaa ttggcggcgc acttgtcgtt
     3061 gcacacgctc ttgcccgcgg ggtccttgtc gaaggtatac aacgtcatgc ccgccggat
//
LOCUS       DBNGWZ010000646         7272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_364165, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000646 DBNGWZ010000000
VERSION     DBNGWZ010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_364165"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16CX_20230"
     CDS             complement(<1..593)
                     /locus_tag="AC16CX_20230"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014867.1"
                     /translation="MKLRYVQLAIGGIGIALVFHAVSGQSAAPANGRYPARPIRAIVG
                     FVPGGATDIIARQLGQKLTESLGQQVIVDNRPGGSGVIAAVMARDAPPDGHTLFFGTI
                     STLATSVATNRKLPYDPLRDFAPITLTLSNPYFLVVHPGVPAASVKEFIALARTRPGQ
                     FNYSSSGTGGGAHLAVELFRTMAGFDMVHIPYKGSAQA"
     gene            940..1920
                     /locus_tag="AC16CX_20235"
     CDS             940..1920
                     /locus_tag="AC16CX_20235"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014868.1"
                     /translation="MLHYRKPLIVALLALLLLSSVAVWAQVYPARPVRVVVGFGAGGP
                     DTIARVVGQQLSAQTGQPFVVDNRPGANGIIGADLVAKATPDGHTLLVTSASFVVNPS
                     VQRRLPFDPVKDFMPISQLTEGEAHILVVNPSVPARNVKDLIALARQPGAKLAYGSPG
                     VGNTIHLVSEVFNSRAGTKIVHVPYKGAGPAITALLAGEIQVMFATAPLSLPHIKAGK
                     LRALAYNHPTKRASFLPDVPTMIESGLSGTEMPPSWHGMLAPAKTPGTVLAKLSAEVQ
                     KALKDAQVRERIVSLGLEPVGSTPDAFRQLVVSNIKRFREVARLAGVKPE"
     gene            complement(2016..3194)
                     /locus_tag="AC16CX_20240"
     CDS             complement(2016..3194)
                     /locus_tag="AC16CX_20240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3014869.1"
                     /translation="MDRTRSGELFLNIGHALDHLFLLIYPTVVLAMAPEFGRPYSEML
                     PLALGGFVAFGAGSLPAGWIADRWSRRGMMIVFFTGVGAASILTGLTRSPWQIAVAIT
                     LMGLFGAIYHPVGIAMLVSGRDKVGRVLGVNGVYGNLGVAFAALVAGALAYWINWRAA
                     FIIPGIVSIAIGIAFAALVPDARISSKRASSGGEGGFPRGLLVRVFAVLTVTTIFGGV
                     IFHSTTVAMPKLFDERLTALVSTTLGIGILVSLVYTFAAMAQLVVGHFLDRGAIKPVL
                     VSVVGFQVPLLVAAAFFDNYVMLVVALAMMFFVFGQIPINDAMVAAYTDEAWRSRVYA
                     VRYVVSFGASACALPLVALTHRYSSDFKYLFFTLAAMAVAMFTAAVLMPARVQTKSTV
                     "
     gene            complement(3419..4411)
                     /locus_tag="AC16CX_20245"
     CDS             complement(3419..4411)
                     /locus_tag="AC16CX_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3014870.1"
                     /translation="MEPFKAYRIFSESDGRIAGRVVNARIDELSAGEVVFRTAYSSVN
                     YKDALAATGMGGRIIRSYPRIGGIDATGTVVSSGDSRFKAGDEVICTSYDFGVGHDGG
                     YSQVCRAPAGWIVPLPMGLTLYEAAAFGVAGYTAGLAVELLELNGMAPGNGNVLVNGA
                     TGGVATLAIDMLARLGYRVVAVTGKDAEHDFLKKLGAAEVLSRQKLEMGTRPLERAQW
                     AAAFDSAGGEQLAWLTRTMQPEGLIASFGNAGGIEFTTTVLPFILRGVRLIGVNSGDT
                     PMPLRRKIWNRLATDLKPRHLDDIAQVIALEDLPGYFDKMLKGKIRGRAVVKLI"
     gene            complement(4424..5587)
                     /gene="zapE"
                     /locus_tag="AC16CX_20250"
     CDS             complement(4424..5587)
                     /gene="zapE"
                     /locus_tag="AC16CX_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR3014871.1"
                     /translation="MAESAFDSAEYTEPARALPVTVLELYQEALRKRGFSPDDSQYAA
                     VLRLQRFYEEWTDYKRRRRTALHRLVVRPPLPRGVYLWGAVGRGKTFLMDSFYRCLPL
                     VRRRRVHFHHFMRDVHRELDELKGTVDPLEALAARIAKRYRLICFDEIALSDVADAMI
                     LGRLLTRTLDRGVVYCMTSNYHPDELYKDGLKREDFLPTIELIKARLDVIRVDSGVDY
                     RRRALQRLRVYHTPLAPETDRALMEAFRQIAEVEEEFHKLDVEGRVIPYRHRAGGVVW
                     FDFKVICGWVRSQNDYIDLAKRFHTLIVSGVPRMGLDRADEARRFTLLVDVLYDQRVK
                     LVVSAEAPPEGLLARDEGTGDARLQAMMFQFDRTASRLTEMQTREYLDQPRGG"
     gene            complement(5594..7024)
                     /gene="lpdA"
                     /locus_tag="AC16CX_20255"
     CDS             complement(5594..7024)
                     /gene="lpdA"
                     /locus_tag="AC16CX_20255"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3014872.1"
                     /translation="MAKTFDVVVIGGGPGGYVAAIRAAQLGLQTACIDEWATPEGKPA
                     LGGTCTNVGCIPSKALLQSSENYEHASHAFAEHGIQVKGLALDLAQMLKRKDKVVRQN
                     NDGIVYLFKKNKVSFFHGRGSFTGGGDGAWQLKATGKSEETITAKHVIVATGSNPRAL
                     PGAAFDNKLVLDNAGALALPEVPRKLGVVGAGVIGLEMGSVWRRLGSEVTILEMLDTF
                     LPAADEAVAKEAWKVFTKEQGLDIVLGANIARAAADKKGVTVEYSTQGGGERKLECDR
                     LIVSIGRVPNTQGLGAEAVRLKLDQRGFIEVDGDCRTNLPGVWAIGDVVRGPMLAHKA
                     EEEGVAVAERIAGHHGHVDFNTIPWVIYTAPEIAWVGKTEQQLKSEEVQYRAGQFPFM
                     ANGRARALGDTRGFVKVLADAVTDRILGVHIIGPMASEMIAEAVVAMEFHAAAEDIAL
                     ICHAHPSLSEAMKEAALGVAKRSLNL"
     gene            complement(7133..>7272)
                     /locus_tag="AC16CX_20260"
     CDS             complement(7133..>7272)
                     /locus_tag="AC16CX_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR3014873.1"
                     /translation="QVVIRPINYLALSYDHRIIDGREAVLSLIAIKEALEDPARMLLD
                     I"
BASE COUNT         1321 a         2420 c         2265 g         1266 t
ORIGIN      
        1 gcctgagcag agcccttgta tggaatgtgc accatgtcga atccggccat ggtccggaac
       61 agctccaccg ccaagtgcgc cccgcctccc gttcctgaag aagaataatt gaactgtccc
      121 ggccgcgtcc tggcgagggc gatgaattcc ttcaccgacg cggcaggcac gcccggatgc
      181 accaccagga aatacggatt ggaaagcgtc aacgtgatgg gggcgaagtc ccgcagcggg
      241 tcatagggga gcttccggtt cgtcgcgaca ctggttgcga gcgtgctgat ggtgccaaag
      301 aaaagcgtgt gcccgtcggg cggcgcgtcc ctggccatga ccgcagcaat gacaccgctg
      361 ccgccgggac gattatccac gatgacctgt tggccgagac tttcggtcag cttctgcccc
      421 agctgtcggg cgatgatatc ggtggcgccg ccgggaacga aacccacgat cgcgcgtatt
      481 ggcctcgcgg gataacgccc gttggcgggg gcggccgatt gccccgaaac cgcgtggaac
      541 acaagcgcga tgccgattcc tccgatggca agttgcacgt atcgcagctt catgcgcgag
      601 cttccctcga gcccccgaca tgatacccgc aggatgagac agtttgacag ccgttgacgc
      661 tcaaactacc ctcccctagc agccagccag acttgagtgt ccggctggct gctaacagca
      721 aaaccgcctc ccagttttca ggacgaacct gcaggattaa tgaccgtatt cagtctgcga
      781 tgcgacatca gaatcagaat gaaatgaatt tgtgaatgtt ttcttacggg cggttttgca
      841 ttaggagttt gtcggagtta agtccggcaa actcctagat cgaaatgatc tgcgcggcga
      901 caggcaggcg cgctgcgcca ctggcatcga gggggaaaca tgctgcacta tcgcaagccg
      961 ctgatcgtgg ctttgcttgc gcttctactc ctgtcatctg tggccgtgtg ggcccaggtc
     1021 tatcccgctc gtcccgtgcg cgtcgtggtc ggttttggag ccggggggcc cgacacgatc
     1081 gcgagagtcg tcggccagca gctttccgcg cagacggggc agccgttcgt ggtggacaac
     1141 cgacccggcg ccaacggcat catcggcgcc gacctcgtcg cgaaagccac acccgacggc
     1201 cacacgttgc tcgtgacctc ggcttcgttc gtcgtcaatc ccagcgtcca gcgcaggctt
     1261 ccgttcgacc cggtcaagga cttcatgccg atctcgcagc ttaccgaggg cgaggcccac
     1321 atactcgtcg tcaatccgtc ggtgcccgcg cgcaacgtca aggatctgat cgcgcttgcc
     1381 agacagcctg gcgccaagct cgcctacggc tcgccgggcg tcggcaatac catccatctc
     1441 gtcagcgagg tgtttaacag ccgggccggg acgaagatcg tccatgtgcc ttacaagggg
     1501 gccgggcccg cgatcacggc tttgctggct ggagaaatcc aggtcatgtt cgcgaccgcc
     1561 ccgctgtctt tgccgcacat caaggcgggg aagctccgcg cgctcgcata caaccatcca
     1621 accaagcgcg catcgttcct gcccgatgtt ccgacgatga tcgaatcggg tctctcgggc
     1681 accgagatgc cgcccagctg gcacggcatg ctggcgcctg cgaagacacc cggtacggtc
     1741 ctggcgaagc tgtcggccga ggtgcaaaag gccctcaagg atgcccaggt gcgggaacgc
     1801 atcgtatcgc ttggactcga gcccgtcggc agcacgcccg atgccttccg gcagctggtc
     1861 gtatccaata tcaagcgctt cagggaggtc gcgcgccttg cgggcgtcaa gccggaataa
     1921 ggacagcttt cagccctcaa cgatcagctt tcagccttga accgataacc atgcatcgca
     1981 gctgatcgct gacagctggc cgctgaaggc tgcaatcaga cggtgctctt tgtctgaacc
     2041 cgggcgggca tcagcacggc ggcggtgaac atggccaccg ccatcgccgc gagcgtgaag
     2101 aacaggtact tgaagtcgct gctgtagcgg tgcgtgaggg ccaccagcgg caacgcgcag
     2161 gcgctcgctc cgaacgagac cacgtagcgc accgcgtaca cgcgcgagcg ccacgcctcg
     2221 tcggtgtaag ccgccaccat ggcatcgttg atcgggatct gcccgaatac gaagaacatc
     2281 atggcaagcg ccaccaccag catgacgtag ttgtcgaaga aagccgccgc gaccagcaac
     2341 ggtacctgaa aaccgactac cgacaccagc accggcttga tcgcaccgcg gtcgagaaaa
     2401 tggccgacca cgagctgcgc catggctgcg aaagtataga ccagcgacac caggatgccg
     2461 atgccgagcg tggtgctgac gagcgcggtg agacgctcat cgaagagctt gggcatggcg
     2521 acggtcgtgg aatggaaaat gacgccgccg aagatggtgg tgacggtcag gaccgcaaag
     2581 acccgcacca gcaggccgcg cggaaaacca ccctcgccac cggaagacgc tcgcttgctg
     2641 ctgatgcggg catctggcac cagggcggcg aacgcgatgc cgatcgcgat cgacacgatc
     2701 cccgggatga tgaacgccgc ccgccagttg atccagtaag caagcgcgcc ggccacgagc
     2761 gcggcaaaag ccacgccgag gttgccgtag acgccgttca cgccgagcac cctgcccacc
     2821 ttgtcgcgtc cgctcaccaa catcgcgatg ccgaccgggt ggtaaatggc cccaaacagg
     2881 cccatcaggg tgatcgccac ggcgatctgc cagggcgagc gggtgagccc cgtcagtatg
     2941 gaggctgcgc cgacgccggt gaaaaacacg atcatcatgc cgcgccggct ccagcggtcg
     3001 gcgatccagc cggcggggag tgatccggcg ccgaacgcga cgaatccgcc cagcgcgagc
     3061 ggcagcatct cgctgtaggg ccggccgaac tccggcgcca tggcgagcac taccgtcgga
     3121 tagatcagca ggaacaggtg gtcgagcgcg tgcccgatgt tgaggaacag ctcgccgctg
     3181 cgggtacggt ccaactcttg tccccctttc cagccccgat tatccggcct gaatggctgt
     3241 cgcgagtata acccgctcga cttcaacgcg acgccatggc tgcttgaatc tgcggccacg
     3301 gaaatgctga tacgcgcccg cttctccaac tgcgtgagta gtgagtggtg aatggaccga
     3361 acgccgccgg aatcgaattt gtccactcac tattcacaac tcaccattca cggctttctc
     3421 atataagctt gaccactgcc cggcctcgga tcttcccctt cagcatcttg tcgaagtatc
     3481 cgggcaaatc ctcgagcgcg atcacttgcg cgatgtcgtc gaggtgccgg ggcttcaaat
     3541 cggtggcgag ccggttccag atctttcgtc gcagcggcat cggcgtgtcg ccggaattga
     3601 ccccgatcag gcgcacgccg cgcaggatga agggcagcac ggtggtcgtg aactcgatgc
     3661 cgccggcgtt gccgaaactc gcgatcagcc cctccggttg catcgtgcgg gtgagccagg
     3721 ccagttgctc gccgcccgcc gaatcgaacg ccgccgccca ctgcgccctt tccagcggcc
     3781 gcgttcccat ctccagcttc tgccgcgaca gcacctcggc tgcaccgagc ttcttgagaa
     3841 agtcatgctc ggcatccttt cccgtgactg caacaacgcg atatcccagc ctcgccagca
     3901 tgtcgatggc gagcgtcgcc acgccaccgg tcgcgccgtt gaccagcacg tttccgttgc
     3961 cgggcgccat gccgttcaac tccagcagct ccaccgcgag tccggccgta tagccggcga
     4021 cgccgaaagc cgccgcttcg tagagcgtca gccccatggg cagcggcacg atccagccgg
     4081 cgggcgcgcg gcagacctgc gaataaccgc cgtcgtgacc gacgccgaaa tcgtagctgg
     4141 tgcagatcac ctcgtcgccg gccttgaagc gggaatcgcc ggacgaaacc accgtgccgg
     4201 tcgcgtcgat gccgccgatg cgggggtagc tccgtatgat cctgccgccc atgccggtgg
     4261 ccgccagcgc gtccttgtag ttgacgctcg agtacgcggt gcggaacacc acctcgcccg
     4321 ccgacaactc gtcgatccgg gcgttcacga cccgtccggc gatgcggccg tcactctcgc
     4381 tgaagatgcg gtaggccttg aagggctcca tggttgcgtt tccctatcct ccgcgcggct
     4441 gatccaggta ttcccgcgtc tgcatctcgg tcaggcggct cgcggtgcgg tcgaactgga
     4501 acatcatggc ctgcagacgg gcatcgccgg taccctcatc ccgcgcgagc aacccctccg
     4561 gcggcgcctc ggccgatacg acaagcttca cgcgctggtc gtagagcacg tcgacgagca
     4621 gcgtgaagcg gcgcgcctcg tcggcgcggt cgagtcccat gcgcggcacc cccgagacga
     4681 tgagggtgtg gaagcgcttg gcgaggtcga tgtaatcatt ctgcgaacgg acccagccgc
     4741 agatgacctt gaagtcgaac cacaccacgc ctccggcccg gtggcggtag ggaatgacgc
     4801 gcccttcgac gtcgagcttg tggaactcct cctcgacctc ggcgatctgc ctgaacgcct
     4861 ccatcagcgc ccggtcggtc tccggcgcga gcggcgtgtg gtagacccgg agcctctgca
     4921 atgctcgcct gcggtagtcg acgccgctgt ccacgcggat cacgtcgagc cgcgccttga
     4981 tcaattcgat cgtcggcagg aaatcctcgc gcttcagccc gtccttgtaa agctcgtccg
     5041 ggtggtagtt cgacgtcatg cagtagacca cgcctcggtc aagagtgcgc gtcagcagcc
     5101 gtcccaggat catcgcgtcc gcgacgtccg acagcgcgat ctcgtcgaag cagatcaggc
     5161 ggtagcgctt ggcgatgcgt gcggcgagcg cttccagcgg atccaccgta cccttcagct
     5221 cgtcgagctc gcggtgcacg tcgcgcatga aatgatgaaa atggacgcgg cgccggcgca
     5281 cgagcggcag gcagcggtag aagctgtcca tgagaaacgt cttgccgcgg ccgaccgcac
     5341 cccacaggta gaccccgcgg ggcagcggcg ggcgcaccac caggcggtgc agcgcggtcc
     5401 ggcgcctccg cttgtaatcg gtccattcct cgtagaaccg ctgcaggcgc agcacggcgg
     5461 catactgcga gtcgtccggc gagaagcccc tttttctgag cgcttcctgg tagagctcga
     5521 ggacggtgac gggaagcgct ctggccggct cggtgtactc ggccgagtca aaggcgcttt
     5581 cagccatgag cgatcagaga ttcaagctgc gcttggcaac gccaagcgcg gcttccttca
     5641 tcgcctcgga gagcgaggga tgcgcatggc agatgagcgc aatgtcctcc gcagcagcat
     5701 ggaattccat cgcgaccacc gcttcggcga tcatctcgga tgccatcggc ccgatgatgt
     5761 gaacgccgag gatgcggtcg gtcaccgcgt ccgcgaggac cttgacgaag ccgcgggtgt
     5821 cgcccagcgc gcgcgcccgc ccgttcgcca tgaacgggaa ctggccggcc cgatactgca
     5881 cctcttcgct cttcagttgc tgctcggtct tgccgaccca ggcgatctcg ggcgcggtgt
     5941 agatcaccca cggaatggtg ttgaaatcca cgtgcccgtg gtgtccggcg atacgctcgg
     6001 ccaccgcgac gccctcttcc tccgccttgt gcgcgagcat cgggccgcgc acgacgtcgc
     6061 cgatcgccca cacgccaggg aggttggtgc ggcaatcgcc atccacctcg atgaagccgc
     6121 gctggtcgag cttgagcctg accgcttctg cgcccagccc ctgcgtgttc ggcacgcggc
     6181 cgatcgagac gatcaggcgg tcgcattcga gcttccgctc cccgcccccc tgggtcgagt
     6241 actcgacggt gacgcctttc ttgtcggctg ctgcccgggc aatgttcgcg ccgagcacga
     6301 tgtcgagacc ctgttccttg gtgaacacct tccaagcttc cttggccacc gcttcgtccg
     6361 ctgccggcag aaaggtgtcc agcatctcga gtatcgtcac ctcggagccg agccggcgcc
     6421 acacgctgcc catctcgagg ccgatcacgc cggcgccgac tacgccgagc ttcctcggga
     6481 cctcgggtag cgcgagcgcg ccggcgttgt cgaggacgag cttgttgtcg aacgcggcgc
     6541 ccggcagcgc ccgcgggttg gagccggtcg cgacgatgac gtgcttcgcc gtgatcgtct
     6601 cttcgctctt cccggtcgcc ttgagctgcc acgcgccgtc gccgccgccg gtaaagctgc
     6661 cgcgcccgtg aaagaagctc accttgttct tcttgaagag atagacgatc ccgtcgttgt
     6721 tctggcgcac caccttgtcc ttgcgcttga gcatctgcgc gagatcgagc gcgagcccct
     6781 tcacctggat gccgtgctcg gcgaaggcgt gggaggcgtg ctcgtagttc tcggacgact
     6841 gcagcagcgc cttcgaagga atgcagccga cgttggtgca ggtgccgccg agcgccggct
     6901 tgccctcggg ggtggcccac tcgtcgatgc aggcggtctg cagcccgagc tgcgcggcgc
     6961 ggatcgcggc gacgtagccc cccggcccgc cgccgatcac cactacgtca aacgtcttcg
     7021 ccataactca caaaagcata gacgcaaagg cacaaagaac gcaaagaagg gctgtacgaa
     7081 taatgatctt ctcgtcgcaa tctttgcggc ttagcggctt tgcgtcgaat atttagatgt
     7141 cgagcagcat gcgggcggga tcctccagcg cttccttgat cgcgatcagg gagagcaccg
     7201 cctcgcgccc gtcgatgatg cggtggtcgt aggacagcgc gaggtagttg atcggccgga
     7261 tcaccacctg gc
//
LOCUS       DBNGWZ010000647         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2254005, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000647 DBNGWZ010000000
VERSION     DBNGWZ010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2254005"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="AC16CX_20265"
     CDS             <1..969
                     /locus_tag="AC16CX_20265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HIR3014874.1"
                     /translation="GPLENLKLETFPDPRPGPDEVLVEVHAASVNFPDLLVIGGTYQI
                     LPATPFVPGKDLAGTVAATGANVSSVKPGDRVMAQIEYGAFAERVAVPSHSCYTMPDS
                     MSFAEGAAMGLVYLTAHFALVERGGLKAGETVLVTGAAGGVGLATVQLAKALGAIVIA
                     TVSSEQKAAVARAGGADHVVRTDAPGLRERLRLEVFAAVGNRGVDLIVDSVGGDVFDA
                     CLRAIAWCGRLVTVGFASGRIPEVKVGLILVKNISLIGLHVSDYRDRVADKARPAQAE
                     LFRLYEAGKLKPRVMAAYPFEAYREALAAVRDRRVLGKAVLAIRQE"
     gene            1006..>2420
                     /locus_tag="AC16CX_20270"
     CDS             1006..>2420
                     /locus_tag="AC16CX_20270"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014875.1"
                     /translation="MASVLPLKPESPPPMLEEGASLRLCETVSRWRYEDLPPDVVRVL
                     KSFLIDTLGVIGGAARAPGIAELYARLTRWERDGAATALIGKRRLSPPSAALANGTAA
                     HALDFDDQHDPARVHTNCVTLPALLATAEDLGGVSGREFLLAYAVSAELHARLGLACY
                     NSLCKGWHPTMLFGVLAASLGAGRLLKLGAGGLNNALGLAFHQACGSAQSMRDGVLSK
                     RLGPGFAARAAVLGAFLAADGISGTRRTLEGNAGLAALYERNEVRPELLFEGLGREWR
                     VLEYSFKPYPCCRCNHTAIDLGIRLHAEGVRPDAVTSVEIGLGNVNWLTVGEPYDVKR
                     DSVVHAQFNAAYSFARALADGRVDLRSYQRPQIADSAIAALAGRIRVVSDPAIEPTAI
                     EPARVKVTLRDGRATELTATVMKGSPQQPLTDPELLAKFHDCLEFGLGASRAEAERLA
                     QAVAGLERSTDVARDIVDAFPL"
BASE COUNT          366 a          826 c          844 g          384 t
ORIGIN      
        1 ggaccgctgg aaaacctgaa gcttgaaacg tttcccgacc cccggccggg gccggatgag
       61 gtgctggtcg aggtccacgc cgccagcgtc aacttccccg atctcctcgt gatcggcggc
      121 acctaccaga tcctgccggc gacgcccttc gtgccgggca aggatctcgc cgggacggtt
      181 gccgcgaccg gcgcgaacgt ctcctccgtg aaacccggcg accgcgtgat ggcgcagatc
      241 gagtacggag cctttgcgga acgggtggcc gtcccgtctc acagctgcta caccatgccc
      301 gacagcatga gttttgccga aggtgcggca atggggctcg tctatctcac cgcgcacttc
      361 gcgttggtcg agcgcggggg gctgaaggcc ggcgagaccg tgctggtgac cggcgcggcc
      421 ggcggcgtcg ggctcgccac cgtgcagctc gccaaggcgc tcggcgcgat cgtcatcgcc
      481 accgtcagca gcgagcaaaa ggccgcggtt gcgcgcgcgg gcggcgctga tcacgtggta
      541 cggacggatg cgcccggcct gcgcgagcgc ttgcgcctcg aggtattcgc cgcggtgggc
      601 aaccgcggcg tggacctgat cgtggattcc gtcggcggcg acgtgttcga cgcgtgcctg
      661 cgcgccatcg cctggtgcgg gcgccttgtc accgtgggct tcgcctccgg ccgcataccg
      721 gaagtgaagg tcgggctcat cctggtgaag aacatctcgc tgatcgggct gcacgtgagc
      781 gactatcgcg accgcgtggc ggacaaggcg cgcccggccc aagcggaact gttccggctg
      841 tacgaggcgg gcaagctcaa gccgcgcgtg atggcggcct atccgttcga ggcttaccgc
      901 gaagcgctgg cggcggtgcg cgaccgccgg gtgctcggca aagcggtgct ggcaatccgg
      961 caggaatgat ccgacgctgc ggcacgcgct tataatgggc cggccatggc aagcgtgctg
     1021 ccgctgaaac ccgaatcacc ccctcccatg ctggaggaag gcgcaagcct gcgactgtgc
     1081 gagacggtct cgcgctggcg ctacgaggac ctgccccccg atgtcgtgcg cgtcctcaag
     1141 tcgttcctga tcgacacgct gggcgtgatc gggggtgcgg cgcgcgcgcc gggcatagcg
     1201 gagttgtacg cgcgcctgac gcgctgggag cgcgatgggg cggccaccgc gctgatcggc
     1261 aagcgccgct tgtccccgcc gagcgccgcc ctcgccaatg gcaccgcggc gcatgcgctt
     1321 gatttcgacg accagcacga tccggcgcgc gtgcacacca actgcgtgac gctgcccgcg
     1381 ctgctcgcga cggcggaaga cctgggcggg gtttccggac gcgagttcct gctcgcctac
     1441 gccgtgagcg ccgaactaca cgcgcgactc ggtctcgcct gctacaacag cctctgcaag
     1501 ggctggcacc cgacaatgct attcggcgtg ctcgccgcga gcctcggtgc cggccggctg
     1561 ctcaagctcg gcgccggagg cctcaacaac gccctggggc ttgcgttcca ccaggcgtgc
     1621 ggttcggcgc agagcatgcg cgacggggtg ctctccaagc gcctgggccc cggtttcgcc
     1681 gcgcgcgccg cggtgctcgg cgcctttctc gcagcagacg gcatcagcgg cacccggcgc
     1741 acgctggaag ggaacgcggg ccttgccgcg ctctacgagc gaaacgaagt gcgccccgaa
     1801 ttgctgttcg agggcctggg gcgggaatgg cgcgtcctgg aatacagctt taaaccctac
     1861 ccttgctgcc gctgcaacca tacggcgatc gatctcggca tccggctgca cgcggagggc
     1921 gtgcgtccgg acgcggtgac atccgtcgag atcgggttgg gcaacgtcaa ctggctcacg
     1981 gtcggcgagc cctacgacgt caagcgtgat tcggtggtac acgcccagtt caacgcggcg
     2041 tacagtttcg cccgcgctct tgccgatgga cgcgtggacc tgcgcagcta ccagcggccg
     2101 cagatcgccg attccgcgat cgctgcgctc gccgggcgca tccgggtcgt ttcggatccg
     2161 gcaatcgagc ccaccgcgat cgaacctgcg cgcgtcaaag tgacgctcag ggatggacgc
     2221 gccaccgagc tcaccgcaac cgtgatgaaa ggcagtccgc agcaaccgct gacggatccc
     2281 gagctcctcg ccaagttcca cgattgcctg gaattcgggc tgggggcgtc gcgggcggag
     2341 gcggagcgcc tggcacaggc cgtggccggc cttgagcgca gcaccgatgt tgcccgcgat
     2401 atcgttgatg ctttcccgct
//
LOCUS       DBNGWZ010000648        14799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2885201, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000648 DBNGWZ010000000
VERSION     DBNGWZ010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 14799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14799
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2885201"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /gene="rplI"
                     /locus_tag="AC16CX_20275"
     CDS             <1..369
                     /gene="rplI"
                     /locus_tag="AC16CX_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3014876.1"
                     /translation="NYLIPHGKAKRATPANVAEFEQRRAELEQAQADALTKAQEKAAK
                     LDGLMIQITQKAGVDGKLFGSVTNVDISDALKAQGLEVPKAAIRMPQGPLKQVGDYPI
                     KIALYADVVVTVTVSVLGEQ"
     gene            518..3172
                     /locus_tag="AC16CX_20280"
     CDS             518..3172
                     /locus_tag="AC16CX_20280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF005852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3014877.1"
                     /translation="MAQAQVVTSLVKDVQVEAARVPPQSIEAEQSVLGGLLLDNTAWD
                     RVADLVSEQDFYRADHRLIFQHIGELIEHGKPADALTVAESLERSSKLAEIGGHAYLG
                     SLALNTPSAANIRRYAEIVRERSIMRNLAAVGTEIADTAYNPMGRDAKALVDEAEAKV
                     FQIAEAGAKSRQGFVKIDPLLTETVERIDMLYNRENKDEVIGLATGFVDLDRMTSGLH
                     GGELVIIAARPSMGKTALAMNIVEHATLRLKKAVAVFSMEMSGTQLAMRMIGSVGEFD
                     QHELRTGTFKDEDWSRMVDAVGKLNEVQIYIDDSAGLNALEVRSRARRLHRQCGGLSL
                     IVVDYLQLMSASGGEENRATEIAEISRSLKGLAKELNIPVIALSQLNRSVESRQDKRP
                     MMSDLRECVTGDTLVMLASGHRVPIHELVGQTPEVLAVSEDGRIVTAKSDCVWSKGVK
                     PVFKVSLASGRTVRATAEHRLYGPRGWVRVGELQPGSRLGLARRLPEPASGRRWTEAQ
                     LVLLGHLVGDGSYLNHQPLRYATASEENSAAVRTAVEALGSEVKRYAGRGQWHQLLIS
                     GNGDRWHSAGVGKWLKNLGIFGQRSHEKRLPQEVFELPDHQVALLLRHLWATDGSINV
                     RQTSKGAPRVFFSTSSSGLASDMAALLLRFGIVVRIRKTLHSVYRPCYAVDVSGAEHQ
                     RVFVERIGAFGPRAVPALRLKAWLDAREPNTNVDTLPMEAFADVRASMRAHGVTTRGM
                     AALRGTSYGGTSHFRFAPSRAMIADYAEKLGDEDLRRWADTDLFWDRVVAVEPDGEEE
                     VFDLTVPGPSSWLADGIVSHNSGAIEQDADLILFIYRDEVYNPDTQKRGIAEIIVAKQ
                     RNGPTGVFNLTFLGKHILFKNYADPGRF"
     gene            complement(3243..4679)
                     /locus_tag="AC16CX_20285"
     CDS             complement(3243..4679)
                     /locus_tag="AC16CX_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3014878.1"
                     /translation="MIQRKFASSSSSVVARFPAATKLFVLDTNVLMHDPTSLFRFEEH
                     DLYVPMSTLEELDNNKKGMSEVSRNARQASRYLEEMITRCDHSISEGIALEVHGDKTA
                     TGRLFLQTEAINGELPGRLASGKSDNQILAVVKHLQELHPRRQVILVSKDINMRIKAR
                     ALGLAAEDYFNDKVLEDTDLLYTGARELPLDFWESHGREMESWQQHGHTYYRVRGPLC
                     PKLLVNEFVCLEADRPFHAQVKEVSGKTAILMMLRDYAGQRHNVWGVTARNREQNFAL
                     NILMNPQIDFVTLLGQAGTGKTLLTLAAGLMQTLEHKIYTEIIMTRVTVPVGEDIGFL
                     PGTEEEKMNPWMGALEDNLDVLNKTDDEAGEWGRAATRDLIRSRIKVKSLNFMRGRTF
                     INKYLIIDEAQNLTPKQMKTLVTRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDR
                     MKGWSHSGHVTLTRGERSRLADYAAEAL"
     gene            complement(4773..5231)
                     /locus_tag="AC16CX_20290"
     CDS             complement(4773..5231)
                     /locus_tag="AC16CX_20290"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004538223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3014879.1"
                     /translation="MLDQPAPDFELPATGGKSFRLSRAKGSPLVLYFYPKDNTPGCTT
                     EGQQFRDLYPEFQKLGCPIYGASRDSMKSHESFKAKMRFPFELLSDAEETVCKLYDVI
                     KMKSMYGRKVRGIERSTFVIDAKGIVRREWRGVKVPGHVQEVLESVKTLK"
     gene            complement(5413..6291)
                     /locus_tag="AC16CX_20295"
     CDS             complement(5413..6291)
                     /locus_tag="AC16CX_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3014880.1"
                     /translation="MKLALRIEVATLRGTREGVPQLMSLLKEHRAGATFLFSLGPDHT
                     GRALGSLPRVPKLKCYGLASLLSGTLLPGADIGSRCTEVMRAIRAEGFEAGILAYDRA
                     DWVRHAARAGESWTVAAMRRARERYEEIFGTPALTHGAPGWQMNPYAYRYTQRLGFRH
                     CSDTRGSGPFIPIVNGEIIACPQLPTTLPTLDELLAGGLNPDEAVRRLLELTRKPPPD
                     GHVYTLRAEVEGTLFAPVLRALLADWRGLGHDPVSLHEYAAGLDLAHLPRRVVVEQPV
                     PGAHGMVAAEGKEFLA"
     gene            complement(6406..7455)
                     /gene="mnmH"
                     /locus_tag="AC16CX_20300"
     CDS             complement(6406..7455)
                     /gene="mnmH"
                     /locus_tag="AC16CX_20300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HIR3014881.1"
                     /translation="MAVTVAQLPEFDEIIDVRSGGEFAEDHVPGAINCPVLNDTERAR
                     IGTIYKQRSSFEAKKIGAALISANIAQHLNERFLDRPPGWRPLVYCWRGGSRSNAFAT
                     VLHQIGWRVGRLDGGYQAYRRAVVSDLERSPHRLRWRVVCGLTGTGKSRLLQAIHDAG
                     GQVLDLEALASHRGSVLGGLPDVPQPTQKMFESRLWKALGGFDPARPVYVEAESRKIG
                     ALRLPGALVEAMWAADCLVLESPLGTRVELLKQEYAHFLDQVEALTAKLERLVPLHGH
                     AVVGKWKALALERNWDELTADLLVRHYDPAYTRAIAKHYTALGRARRFELRDTSDASL
                     EALAQLCLDSETVTE"
     gene            7567..8625
                     /gene="selD"
                     /locus_tag="AC16CX_20305"
     CDS             7567..8625
                     /gene="selD"
                     /locus_tag="AC16CX_20305"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7627..7629,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR3014882.1"
                     /translation="MNKPRETTPIRLTQFSHGGGUGCKLPPAVLGEILKQSPLASAFP
                     DLLVGTETSDDAAVYRLNDRQAIVATTDFFVPIVDDPYDFGRIAATNAISDVYAMGAR
                     PIFALALLGVPMKNFPREAMQSIVAGGHAVCDAAGIPIAGGHSIDSPEPFYGLVALGL
                     IQPDLVKRNDQARAGDVLILGKGLGIGIMGAALKKGELKDKAYRQMLETATQLNTPGI
                     RLSEMPDVHALTDVTGFGLLGHLLEICRGSRLRGDIEFDRLPILSAARHLAEQGYATG
                     ASERNWESCARDVTLPKGMADWKRKLLCDPQTSGGLLVACTAESAGQVLSIFQAQGFK
                     YAREIGQLVAGPVGVTVR"
     gene            complement(8639..9292)
                     /locus_tag="AC16CX_20310"
     CDS             complement(8639..9292)
                     /locus_tag="AC16CX_20310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3014883.1"
                     /translation="MDAATYDAWYRTPRGSWIGDVEYCLLQHMLAPSPGENLLDVGCG
                     TGYFTRRFARDAGQRVTGLDPDRDSLDYARAHGRSNEVYIAGDARELPFPDASFDFVV
                     SVTALCFIQDQRQALQEIVRVTRKRFALGLLNRNSVLYRQKGRNGGTGAYRGAHWHTI
                     SEIDDLLRGLAVRDVLVRTAVFVPSAGAIARVIERLAPNVLPWGAFIAVAGSPSAGS"
     gene            complement(9424..10713)
                     /locus_tag="AC16CX_20315"
     CDS             complement(9424..10713)
                     /locus_tag="AC16CX_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014884.1"
                     /translation="MTGTGIQEKMRDHDSPPRAAARLTAALLVVAWPFSAQAEDIAWD
                     GFVQSHVALRTSNVPCPTTTECDYPAAELRGQLKAEGKSKSGNAAFLGRFDLIRDIAL
                     NETRLETREIYGDWRAEKTAARLGRQVITWGVGDLLFINDTFPKDQVALFTGQPLQYL
                     KLGSDALKLNAYPGSANGELVIASFRSDNIPTSRRFILADPLPIGLPRRTVEPGNGPG
                     ELEISGRVSGYLDNWELAGYASRTHFHSPAFRVTGTEIVGTYPRLNTLGASLTGTLGK
                     GVLSLEAGYYDSPQDRDGRNPSIENSQFRGLIGYSRQLWEDATLGLQLYGEWMRDYAA
                     YRESLPAGFPAKDRVRKVTTVRFTQLFAHQTVTFNLFAFLGLSEEDRYLIPSLRYAFS
                     DNLWAEVGADLFGGKRTGMFGSMQDNSNIYLTVRYAF"
     gene            complement(10710..11504)
                     /locus_tag="AC16CX_20320"
     CDS             complement(10710..11504)
                     /locus_tag="AC16CX_20320"
                     /inference="COORDINATES: protein motif:HMM:NF028441.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HIR3014885.1"
                     /translation="MKSLPQFTLFLLAAISLPAHAADSSTDEIVKRSLEAFYSAGTDM
                     RAKVQMKLINPQGKIRSREMTLLRLNLGQSGDQRYYIYFHGPADVKSTSFMAWKYPAK
                     DSDRWIYVPALKLVKRIAADDKRSSFVGSDFTYEDVSGRNVQDETHSVVRQEDLNGRP
                     TYVVESKPKAQVDYSRRVSWIDRERWLPLKEEYFDARNEPLRTFTADKVEQTGKYWTI
                     TARSMKNLQSGHRTEVVFQEVRYDVGLKPDIFTERYLRDAPVQWVQ"
     gene            complement(11501..13825)
                     /locus_tag="AC16CX_20325"
     CDS             complement(11501..13825)
                     /locus_tag="AC16CX_20325"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3014886.1"
                     /translation="MQKNTLVEFSIAHPKVIFGVALLLTLVFAAQLPRIQLDTNPKNM
                     LPPTSDVRMWNDNVDRTFALYEDTIVVGIVHPKSVLNQGTLEKVRDVTAEILKIKGVA
                     GRDVSSFTTIDNVTAAADGLRVAPLVTRVPATEAEMQALRKTLFDNPLLLDRLISRDE
                     KTTVIYVPLEKGANGKEVADGIREILAKYPGEERYHIAGDPVARDTFGAEMFKLMGIF
                     SPIAGMIMFVAIYAMFRSLALAMTMMMVAMIAIVWTMGLGIGLGFPIHIMNSMTPVFL
                     MAIATDSIHIFNEFYFRYREKQDKRTAIIETMEAVGRPVRYTALATAAGFAVLLFMNV
                     VPVKVFGGMVVFGTVVLRLFSFSLIPAVLTVVREEKMDRAATGEDIAAGRTGRELRRL
                     GAWGADRPKATVAVGLALVVLAILGASRIVVNNNMVTWFKPGSEIRIADAVINRAMGG
                     TSLGYLVAAADQADAIKRPETLRYIEGLQRHLEKLPVVGKTTSVVDYVKRINRVLHDD
                     DPKFDAVPDTAEMVAQYLFLFSMSAKPSDLDNVVDYPFQRANIWVQLKTWDAAAMQSV
                     IRAAEEYQRFNPVALEIKPAGIAYFNLVWSHEVLWDMVKGFLLALAVVFFILAFDFGS
                     LKWALVGSVPLLFTILLIYGVVGYAGKDFDMPISVLSCLSLGMAVDFSIHFVSRLRQR
                     LAEIGADASRDLSALKEALLWTAARPGKGIMRNALLFAAAFSVMLFAPLTPYITVGAF
                     IVSMMLLSALLTLFYLPALIVLTRGRLFKTGDTK"
     gene            complement(13832..>14799)
                     /locus_tag="AC16CX_20330"
     CDS             complement(13832..>14799)
                     /locus_tag="AC16CX_20330"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3014887.1"
                     /translation="AALAKRGIEVVRGNIEKIDPARRAVFVNARELTGDYMIVALGAE
                     LAPESVPGLSAAGHNFYTLAGAESLRDARHKLSKGRVVVLVSAMPFKCPGAPNEAAML
                     LEYDCRRRGIRNEVKIDLYTAEPGPMPVAGAAVSSALRQMIESKGIGYHPNHSVTGVD
                     AALRRITFANGASAEFDLLAYIPPHRAPKVVRDAGMCGESGWVPVDRETMATKFPDVF
                     AIGDVAGIMLTSIGKPLPKAGVFAHYEAEVVANNIVHAITGKGETQRFNGDGECFLEA
                     GDGRAGFGSGNFYAEPAPQITLKPPSIMMHLGKVFYEKFWLYSRF"
BASE COUNT         2712 a         4602 c         4741 g         2744 t
ORIGIN      
        1 aactatctga ttccgcacgg caaagccaag cgcgccacac ccgcgaacgt ggccgagttc
       61 gaacagcgcc gggccgagct ggagcaggcg caggcggatg cgctcaccaa ggcccaggag
      121 aaggcggcga aactcgatgg gttgatgatc cagatcacgc agaaagcggg cgtggacggc
      181 aagctctttg gctccgtcac caacgtggac atctcggatg cgctgaaagc gcaaggtctg
      241 gaagtgccga aagcagcgat ccgcatgccg cagggcccgc tgaagcaggt gggagactac
      301 ccgatcaaga tcgcgctgta tgccgacgtc gtggtgaccg tgacggtttc ggtgttgggg
      361 gaacagtaag gccgcgtaac acaacaaaat tctttagcgc aaaaaaaagg gctggcgaca
      421 gccctttttt cgtttacact tcaccagcaa cgcttccgac gcgcgccgca gccagcggca
      481 ggaaattcac actaacaagc aaccggcaca gcgcatcatg gctcaggctc aggtcgtcac
      541 ctcgctcgtc aaggacgtcc aagtcgaagc tgcccgggtg ccgccccagt cgatcgaagc
      601 ggagcagtcg gtcctgggcg gcttgctgct cgacaatacg gcgtgggaca gggtcgcgga
      661 tctcgtcagc gagcaggact tctaccgcgc ggatcatcgt ctcatcttcc agcacatcgg
      721 ggagctcatc gagcacggca agcccgccga tgcgctgacc gtcgccgaga gcctcgagcg
      781 cagcagcaag ctcgctgaaa tcggcgggca cgcctacctc ggttcgctcg cgctgaatac
      841 cccgagcgcc gccaacatcc ggcgttacgc cgagatcgtg cgcgagcgct ccatcatgcg
      901 caatcttgcc gccgtgggta ccgagattgc ggacaccgcc tacaatccga tggggcggga
      961 tgccaaggcg ctggtggacg aggccgaggc caaggtgttc cagatcgcgg aggcgggggc
     1021 caaatcacgc caggggttcg tcaaaatcga cccgctcctc accgagaccg tcgagcggat
     1081 cgacatgctc tacaaccgcg agaacaagga cgaggtcatc gggctggcga cgggctttgt
     1141 cgacctcgac cggatgactt ccggtctgca cggcggcgag ctggtgatca tcgcggcgag
     1201 gccctcgatg ggaaagaccg cgcttgccat gaacatcgtc gagcacgcga cgctgaggct
     1261 caagaaggcg gtcgcggtgt tcagcatgga aatgtcgggc acccagctcg cgatgcgcat
     1321 gatcggctcc gtcggcgagt tcgaccagca cgagctgagg accggcacct tcaaggacga
     1381 ggactggtcg cgcatggtgg acgcggtcgg gaagctcaac gaggtccaga tctacatcga
     1441 cgacagcgcg ggactcaacg cactggaagt acgctcgcgc gcgcggcggc tgcaccggca
     1501 gtgcggcggc ctgtcgctga tcgtggtgga ctacctgcag ctcatgtcgg cgagcggcgg
     1561 ggaggaaaac cgggccaccg agatcgcgga aatttcgcgc tcactgaagg ggctcgccaa
     1621 ggagctcaac attcccgtca tcgcgctgtc gcaattgaac cgcagcgtcg agtcacgcca
     1681 ggacaagcgg ccgatgatgt cggacctgcg ggaatgcgtg actggggaca cgctggtgat
     1741 gctcgcgagc ggtcatcgcg tccccattca cgagctcgtc gggcagacgc cggaggttct
     1801 cgccgtctcc gaagacgggc gcatcgtaac agcgaagtcc gattgcgtat ggtccaaggg
     1861 tgtgaagccg gtgttcaagg tcagcctcgc cagcgggcga acggtgcgag ccactgcgga
     1921 gcaccggctc tatggtcccc gcggatgggt gcgggtgggt gagctgcagc ccgggagccg
     1981 acttgggctg gcgagacggc tgccggagcc ggcatcgggc cggcgctgga cggaagcgca
     2041 actcgttctg ctcggccact tggtgggcga tggcagctac ctcaatcatc agcccctgcg
     2101 ctacgccacg gcctcggagg aaaacagcgc agcggtgcgc actgcagtgg aggcgctagg
     2161 ctcggaagtg aagcgctatg cgggacgggg ccagtggcac cagctcctga tcagcggcaa
     2221 cggcgatcgc tggcactctg cgggagttgg caagtggctt aagaacctcg gcatcttcgg
     2281 acagcgcagc cacgagaaac gcctgccgca ggaggtcttc gagcttcccg accaccaggt
     2341 cgcgctcctg ctccgccacc tttgggccac cgacggcagc atcaacgtgc gccagacttc
     2401 caagggagcg ccgcgcgtct ttttttccac cagcagctcg ggactggcct ccgacatggc
     2461 ggcgctgctc ctgcgcttcg gtatcgtggt gcgaatccgc aagacccttc attccgtcta
     2521 tcggccgtgt tacgccgtgg acgtttccgg ggcggagcac cagcgggtgt tcgtcgagcg
     2581 catcggggcc ttcgggccac gggccgtacc agcgcttcgg ctcaaggcct ggctcgatgc
     2641 gcgggaaccg aataccaacg tggataccct gccgatggaa gccttcgcgg acgtgcgcgc
     2701 ctcgatgcgc gcgcatggcg tcaccactcg cggcatggcg gccctccggg gcacctccta
     2761 cggcggcacc tcgcacttca ggtttgcacc gtcccgggcg atgatcgccg actacgccga
     2821 gaagctgggc gacgaggacc ttcggcgctg ggcagatacg gatctgtttt gggaccgcgt
     2881 agtagccgtc gagcccgacg gcgaggagga ggtgttcgac cttaccgtgc ctggcccgtc
     2941 ttcttggctc gccgacggta tcgtcagcca caactcgggc gcgatcgagc aggacgcgga
     3001 cctgatcctc ttcatctacc gcgacgaggt ttacaacccg gatacccaga agcggggcat
     3061 cgcggaaatc atcgtcgcca agcagcgcaa cggcccgacc ggggtgttca acctgacctt
     3121 tctcggcaag cacatcctct tcaagaacta cgcggacccc ggccgcttct aatttgccgt
     3181 tagccgtcag cgttcagctg atggccaacc cagtcaccag tcacgccccg cgaatcaccg
     3241 gatcaaagcg cctcggcggc gtagtccgcc aggcgcgagc gctcgccgcg cgtgagcgtg
     3301 acgtgcccgc tgtgcgacca tcccttcatc cggtccacga cgtaggtgag ccccgagctg
     3361 ccttcggtga gatagggcgt gtcgatctgc gcgatgttgc cgaggcacac caccttggtg
     3421 ccgggcccgg cgcgggtgac cagcgtcttc atctgcttcg gcgtcaggtt ctgcgcctcg
     3481 tcgatgatca ggtacttgtt gatgaaggtg cggccgcgca tgaagttgag cgacttgacc
     3541 ttgatgcgcg agcggatcag gtcgcgggtc gccgcgcggc cccactcgcc ggcctcgtcg
     3601 tcggtcttgt tgagcacgtc gaggttatcc tcgagcgctc ccatccacgg attcatcttc
     3661 tcttcctcgg ttccgggcag gaagccgatg tcctctccca ccggcaccgt gacccgcgtc
     3721 atgatgatct ccgtgtagat cttgtgctcg agcgtctgca tgagcccggc ggcaagcgtg
     3781 agcagcgtct tgccggtgcc ggcctggccg agcagggtca cgaagtcgat ctgcggattc
     3841 atcaggatgt tgagcgcgaa attctgctcg cggttgcgcg ccgtgacccc ccagacgttg
     3901 tgacgctggc cggcgtagtc gcgcaacatc atcaggatgg cggtcttgcc gctcacttcc
     3961 ttgacctgcg cgtggaacgg acggtccgcc tcaaggcaga cgaattcgtt caccagcagc
     4021 ttcgggcaca gcggcccgcg cacccggtaa taggtgtgcc cgtgctgctg ccatgattcc
     4081 atctcccgcc cgtggctttc ccagaagtcc aggggcagct cgcgcgcgcc ggtgtagagg
     4141 agatcggtgt cctccagcac cttgtcgttg aaataatcct cggccgccag ccccagtgcg
     4201 cgcgccttga tgcgcatgtt gatgtccttc gacaccagga tcacctggcg cctgggatgc
     4261 agctcctgca ggtgcttcac caccgcgagg atctggttgt ccgatttgcc gctggcgagc
     4321 cggcccggaa gctccccgtt gatcgcctcg gtttgcagga acaggcgtcc cgtcgcggtc
     4381 ttgtcgccgt ggacctcgag cgcgatgccc tccgagatgc tgtggtcaca gcgggtgatc
     4441 atctcctcga ggtaacggct cgcctggcgc gcgttgcgcg acacttccga catgcctttc
     4501 ttgttgttgt cgagttcctc gagagtggac atcgggacgt agaggtcgtg ctcttcgaag
     4561 cggaacaggc tcgtggggtc gtgcatcagc acgttggtgt cgagcacgaa gagcttggtg
     4621 gcggcgggga agcgggcgac tacggaagag gacgaggatg cgaacttgcg ctggatcatg
     4681 tttctcccaa tataagtttc ggtttcatag ttccaagttc aaggtcggaa ccctcggttc
     4741 gcgatccgct ccaaccttga acctgttcgc tgttacttca gggtcttgac ggattcgagc
     4801 acctcctgga catggcccgg caccttgacg ccgcgccatt cgcgtcgaac gattcccttc
     4861 gcgtcgatga cgaatgtgct gcgttcgatg ccgcgcacct tcctgccgta catgcttttc
     4921 atcttgatga cgtcgtagag cttgcacacg gtttcctcgg cgtcggacag cagctcgaac
     4981 gggaagcgca tcttggcctt gaagctttcg tgcgacttca tgctgtcgcg cgatgccccg
     5041 tagatcgggc agccgagctt ctggaattcc gggtaaaggt cgcggaactg ctggccttct
     5101 gtggtgcagc cgggtgtgtt gtctttggga tagaaataca gtacgagagg agagcctttg
     5161 gcgcgggata ggcggaagga cttgccgccg gtggcgggca gctcgaagtc gggtgctggc
     5221 tgatctagca ttcggcgggt cggagcctcg tcgcgtaatg gcctggaaat aatagtgacc
     5281 gaaaccggct tcccgtgcaa gcgtgagaag gcaacagacg ggatttacag gattatttcg
     5341 agaaaatgat gttgctcagt cacgctagtc ctgcaaatcc cgtccgttta tcgttataaa
     5401 gaattcctcg cgtcacgcga ggaattcttt gccctcggcc gcgaccatgc catgcgcccc
     5461 cggaaccggt tgttcgacga cgacccgccg cggcagatgg gcgaggtcga ggcctgccgc
     5521 atactcgtgc aaggagacag gatcatgccc gagcccgcgc cagtcggcga gcagggcgcg
     5581 caagaccggc gcaaacaatg tgccctcgac ttcggcgcgc agcgtgtaaa cgtggccatc
     5641 gggtggcggt ttgcgggtga gctcgagcag ccgccgcacg gcttcgtccg gattgaggcc
     5701 gcctgcaagc aactcgtcga gcgtgggcag cgtggtaggc agttgcggac aggcgatgat
     5761 ctcgccattc actatgggga tgaaagggcc gctgccgcgg gtgtcggagc agtgccggaa
     5821 tcccagccgc tgggtgtatc gatacgcata cggattcatc tgccagccgg gcgcgccatg
     5881 cgtgagagcg ggcgttccga atatctcctc gtatcgctcg cgggcacggc gcatcgccgc
     5941 cacggtccat gattcgcctg cccgggctgc gtgtcgcacc caatccgcgc gatcgtaggc
     6001 caggattccc gcctcgaatc cctccgccct gatggcgcgc atcacttccg tgcagcgcga
     6061 accgatgtcg gcgcccggca gcagtgtgcc cgacaacagc gaggcaagcc cgtagcattt
     6121 caatttcggc acgcgcggaa ggctccccag ggcgcgaccg gtgtggtcgg gcccgagact
     6181 gaacaggaag gtagcgccgg cgcggtgctc cttgaggagg gacatcagtt gcggcacgcc
     6241 ttcgcgtgtg ccacgcaatg tcgcaacctc gatgcgcaaa gcgagcttca taagggcagt
     6301 gattaagtga ctcggtgact aagtgattag gtggctaagt gaccgctgat gacgcggaac
     6361 gcgctcatgt tgtttgggta agcgctcaat cgcttaatca cccaatcact cagttactgt
     6421 ttctgagtcg aggcaaagtt gcgcgagcgc ttcgagagat gcatcgctcg tatcccgcaa
     6481 ctcgaacctc cgcgcgcggc caagcgccgt atagtgcttg gcgatagcgc gggtgtaagc
     6541 cgggtcgtaa tgcctcacga gaaggtcggc ggtaagctca tcccagttcc gttcgagcgc
     6601 gagcgccttc cactttccga cgaccgcgtg gccgtgcaac ggaaccaacc gctccagttt
     6661 tgcggtcagg gcttcgacct gatcgagaaa atgggcgtac tcctgtttga gcaactcgac
     6721 ccgcgtcccg aggggggatt cgagcaccag gcaatcggcc gcccacattg cctcgaccaa
     6781 cgccccgggc aggcgcaacg cgccgatctt ccggctttcc gcctcgacgt aaacggggcg
     6841 cgcgggatcg aatccgccga gcgccttcca gagacggctt tcgaacattt tctgcgtcgg
     6901 ttgcggcacg tccggaaggc cgccgaggac ggatccgcga tgggaagcaa gcgcttccag
     6961 atcgagtact tggccgcctg cgtcatgaat ggcttgcagc aagcggcttt tccccgtgcc
     7021 ggtgagtccg cacacgaccc gccagcgcag ccggtgcggc gaccgttcga ggtccgagac
     7081 caccgcacgg cggtaagcct ggtagccgcc gtcgagccgg ccgaccctcc acccgatctg
     7141 gtgcagcacc gtcgcaaacg cgttgctgcg gctgccgcca cgccagcagt agacgagcgg
     7201 tcgccagccg ggcggccggt cgaggaagcg ctcgttcagg tgctgtgcga tgttggcgct
     7261 gatgagtgcc gctccgattt tcttcgcctc gaacgatgag cgctgcttgt agatcgtccc
     7321 gatccttgca cgctcggtat cgttcagcac cgggcagttg atcgcgccgg gcacgtgatc
     7381 ttcagcgaac tcgcccccgg agcggacgtc gataatctcg tcaaactcag gcagttgtgc
     7441 tacggttaca gccagcggtc ctttcgcggc cgtgggctgc gaagccggat gcgccctcgg
     7501 tgagccggct ttagcagagg cgggcaaatc caccctgatc gtcatcgcgc atagtttacg
     7561 ggaaaaatga acaaacctcg ggaaacgacc ccaatcaggc tcacccagtt ctcgcacggc
     7621 ggcggctgag gctgcaaact accgccagcg gtgctggggg aaattctcaa gcaatcgccg
     7681 ctcgcctcgg cctttcccga tctgctggtc gggaccgaga ccagcgatga tgcggccgtc
     7741 tatcgtttga acgaccggca ggcgatcgtc gcgaccaccg actttttcgt gccgatcgtg
     7801 gacgatccgt acgatttcgg acgcattgcc gccaccaacg cgatttccga cgtctatgcg
     7861 atgggcgcca ggccgatttt cgcgcttgcg ctgctcggcg tgccgatgaa aaactttccg
     7921 cgcgaagcaa tgcagagcat cgtcgccggc ggacatgccg tctgcgatgc cgccggcatc
     7981 ccgatcgcgg gcggccactc gatcgattcg ccggagcctt tttacggctt ggttgcgctt
     8041 ggcctgatcc aacctgatct cgtcaagcgc aacgaccagg cgcgcgccgg cgatgtcctg
     8101 atcctcggca aggggctcgg catcggcatc atgggcgccg cgctcaagaa gggcgagctc
     8161 aaggacaagg cttatcgcca gatgctggag accgcgaccc agctcaatac gcccggcatc
     8221 cgcctctccg agatgccgga cgtgcacgcg ctgaccgatg tcaccggctt cggcctgctc
     8281 gggcacttgc tcgagatctg ccgcggctcg cggctgcgcg gcgacatcga gttcgatcgc
     8341 ctgccgatcc tgtccgccgc gcggcatttg gcggaacagg gctacgccac cggcgcatcg
     8401 gaacgcaact gggagagctg cgcgcgggac gtcacgctgc caaagggcat ggcggactgg
     8461 aagcggaagc tgctgtgcga tccgcagacc agcggcgggc tgctcgtggc gtgcaccgcc
     8521 gaaagcgccg gccaggtgct ttccatcttc caggcgcagg gcttcaaata cgcgcgtgag
     8581 atcggccagc tcgtggccgg gccggtcggc gtcacggtgc gctgatcgca ggagcgcgtc
     8641 agctgccggc agatgggctg cccgcgaccg cgatgaaggc gccccatggc agaacgttcg
     8701 gcgcaagccg ctcgatgacc ctggcgatcg cgcccgccga agggacgaac accgcagtgc
     8761 ggacgagcac gtcgcgcacc gccagaccgc gcagcaagtc atcaatttcg cttatggtgt
     8821 gccagtgcgc gccccggtac gcgccggtgc cgccgttgcg tcctttttgg cgatagagca
     8881 cgctgttgcg attgagcagc cccagcgcga accgcttgcg ggtgacgcgc acgatttcct
     8941 gcagcgcttg tcgctggtcc tgaatgaagc agagcgcggt gaccgatacg acaaagtcga
     9001 aactcgcatc gggaaacggc agttcacgcg cgtctccggc aatgtaaacc tcgttcgagc
     9061 ggccatgcgc ccgggcgtaa tcgagcgaat cacggtcggg atcgaggccg gttacgcgct
     9121 ggccggcgtc ccgtgcaaaa cggcgcgtga aataacccgt tccgcagccg acgtcgagga
     9181 ggttttcacc gggcgacggc gcgagcatgt gctgtaacaa gcaatactcg acatcgccta
     9241 tccagctgcc gcgtggtgtg cggtaccagg catcgtacgt ggcggcgtcc atggcgaggg
     9301 ttcactcaag gtgggcggac aggccagtat ttggtcgcaa gttttcagtt aatgcccggc
     9361 gaggacattc atctcaaccg gtcgcgcgga ttcccaccta taggtgcctg catctgcaag
     9421 cccctagaaa gcatatcgaa cggtgaggta aatgttgctg ttatcctgca tcgacccgaa
     9481 catgccggta cgcttgcctc cgaaaagatc ggccccgacc tcggcccaga gattgtcgct
     9541 gaaggcgtaa cgcagcgaag ggattagata gcgatcttcc tcgctcaggc caaggaaggc
     9601 gaagaggttg aaggtgacgg tctggtgggc aaaaagctgg gtgaagcgaa ccgttgtcac
     9661 cttgcgcacg cggtccttcg cgggaaaacc ggctgggagg ctctcccggt aggccgcgta
     9721 gtcgcgcatc cattccccgt agagctgcaa gccgagagtg gcatcctccc acagttggcg
     9781 cgagtacccg atgagtcccc tgaactggct gttttcgatg gaagggttgc ggccgtctcg
     9841 atcctgcggt gagtcgtagt accccgcctc gaggctgagc acacccttgc ccaaggtccc
     9901 ggtaaggctg gcaccgagag tattaagccg cgggtaggtg ccaacgattt cggtgcctgt
     9961 cacgcgaaag gccgggctgt ggaaatgcgt gcgcgacgcg tagccggcca gctcccagtt
    10021 gtcgagatag ccggagaccc ggcctgagat ttccagctcc ccgggaccgt taccgggctc
    10081 caccgtgcga cgcggcagcc caataggcaa gggatcggcg agaatgaagc ggcggctggt
    10141 cgggatgttg tccgagcgga agctggcgat caccagttcc ccattggccg aacctggata
    10201 ggcgttcagc ttgagcgcgt cgctgccgag cttgaggtat tgcaggggct gaccggtgaa
    10261 cagcgccacc tgatccttcg ggaaggtatc gttgatgaac agcaggtcgc ccacgcccca
    10321 ggtgatcacc tgccgtccca gccgggcggc cgttttctcc gcccgccagt cgccgtaaat
    10381 ctcccgcgtc tcgagccgtg tttcgttcag cgcgatgtcc cggattaaat cgaaacggcc
    10441 gaggaacgcg gcgttccccg acttgctctt gccctcggcc ttgagctgcc cgcgcagctc
    10501 ggccgcggga taatcgcact cggtcgtcgt cgggcagggc acgttgctgg tgcgtagggc
    10561 gacgtgcgac tgcacgaatc cgtcccacgc gatgtcttct gcttgcgcgc tgaagggcca
    10621 cgcgaccaca agcagcgcgg ctgtcagtct cgccgcggca cgcggcggac tgtcgtggtc
    10681 acgcatcttt tcctgaatgc ctgttcccgt cattgcaccc actgcacggg tgcatcccgc
    10741 aggtaacgct cggtgaagat gtcaggcttc aggcctacgt cgtatcgcac ttcctggaac
    10801 acgacctccg tgcggtggcc gctctgcagg ttcttcatac tgcgggcggt gatcgtccag
    10861 tatttgcccg tctgttccac cttgtcggct gtgaaggtgc gcagcggttc gttgcgcgcg
    10921 tcgaagtact cttccttgag cggtagccag cgctcgcgat cgatccagga tacgcgccgc
    10981 gagtagtcca cctgcgcctt gggcttgctt tcgacgacgt aagtcgggcg cccgttcagg
    11041 tcttcctggc gcacgacgct atgggtttca tcctgtacat tgcgtcccga aacgtcctcg
    11101 taggtgaagt cggagccgac gaaagacgag cgcttgtcgt ccgccgcgat gcgcttcacg
    11161 agcttcagcg ctggcacgta aatccagcgg tcgctgtcct tggccggata tttccacgcc
    11221 atgaacgacg tgctcttcac gtcggctgga ccgtggaagt agatgtagta acgctggtcg
    11281 cccgattggc cgagattgag ccgcaacagg gtcatctcgc ggctgcgaat cttgccttga
    11341 ggattgatga gcttcatctg caccttggcg cgcatgtcgg tgccggccga atagaacgcc
    11401 tccagcgagc gtttcacgat ctcgtcggtg gatgaatcgg cggcgtgcgc gggcagcgaa
    11461 attgcggcga gaaggaacag cgtgaattgc ggtaacgatt tcatttcgtg tctcctgtct
    11521 taaacagccg tcctctcgtc agcacgatca gggccgggag gtaaaacagg gtgagcaggg
    11581 ccgagagcag catcatgctg acgatgaagg cgcccacggt gatgtagggg gtaagcggcg
    11641 cgaacagcat gaccgagaaa gccgccgcga agagtaatgc gttgcgcatg attcccttgc
    11701 cgggacgggc cgccgtccac agcagcgcct ccttgagcgc ggaaaggtcg cggctggcgt
    11761 cggcgccgat ttccgcgagc cgctgcctca accggctgac gaagtggatc gagaaatcca
    11821 ccgccatgcc gagcgacagg cacgacaaca cggagatagg catgtcgaag tccttgccag
    11881 catagccgac gaccccgtaa atcagcagga tggtgaagag cagcggcacg gaaccgacga
    11941 gcgcccactt gagcgagccg aaatcgaagg cgaggatgaa gaacaccact gccagggcga
    12001 gcagaaagcc cttcaccata tcccacagga cctcgtggct ccataccagg ttgaagtagg
    12061 cgattccggc gggtttgatc tccagcgcca cgggattaaa ccgctggtat tcctccgcgg
    12121 cccggatcac gctttgcatt gccgcggcgt cccaggtctt gagttggacc cagatgttgg
    12181 cgcgctggaa gggataatcc accacattgt ccaggtccga gggcttggcc gacatgctga
    12241 acaggaacaa gtattgggcg accatctcgg cggtatcggg cactgcgtcg aacttcgggt
    12301 cgtcatcgtg cagcacgcgg ttgatgcgct tgacgtagtc gaccacggac gtggtcttgc
    12361 cgacgacggg gagcttctcc aggtgccgct gcaggccctc gatgtagcgc agggtctccg
    12421 gccgcttgat ggcatcggcc tggtcggcgg cggcgaccag atagccgagc gaggtgccac
    12481 ccatcgcccg gttgatcacg gcgtcggcga tgcggatctc gctgcccggc ttgaaccagg
    12541 tcaccatgtt attgttcacc acgatgcgac tggcgccgag aatggcgagc acgaccagcg
    12601 ctaggccgac tgcgaccgtc gcctttgggc ggtcagcccc ccaggcgcca agccgcctca
    12661 gctcccgccc tgtacggccc gcggcaatgt cctcgcctgt ggcggcgcgg tccatcttct
    12721 cctcccgcac gacggtgagg acagcgggga tcaggctgaa gctgaagagc cgaagcacga
    12781 cggtgccgaa aacgaccatt ccgccgaaca ccttgaccgg cacgacgttc ataaacagca
    12841 gcacggcgaa gccggccgcg gtggcgagcg ccgtataacg caccgggcgt cccaccgctt
    12901 ccatcgtttc gatgatggcg gtccgcttgt cctgcttttc acgataacgg aagtagaact
    12961 cgttgaagat gtggatgctg tcggtggcaa tggccatcag gaataccggc gtcatcgagt
    13021 tcatgatgtg aatcgggaat ccgaggccga tgcccaggcc catggtccac acgatcgcga
    13081 tcatggcgac catcatcatg gtcatggcca gcgccaggct ccggaacatg gcatatatag
    13141 cgacgaacat gatcattccc gcgatcggtg agaaaatgcc catcagcttg aacatctcgg
    13201 ccccgaaggt gtcgcgcgct acaggatcgc cggcgatgtg gtagcgctcc tcgcccggat
    13261 acttggcaag aatctcgcgg atgccgtcgg cgacctcctt gccgttggcg cctttttcca
    13321 gcgggacgta gatgacggtg gtcttttcgt cccgcgaaat gaggcgatcc agcaagagcg
    13381 gattgtcgaa cagcgtcttg cgcagcgcct gcatctcggc ttcggtcgcc ggcaccctgg
    13441 tcaccagcgg cgccaccctg agcccatcgg ctgcggccgt gacgttgtca atggtggtaa
    13501 agctggacac gtcacgcccg gccacgccct tgatcttcag aatctcggcc gtcacgtcac
    13561 gcactttttc aagcgtacct tgattcagca cactcttggg gtggacgatg ccgacgacga
    13621 tagtgtcctc gtagagggca aaggtccggt cgacgttgtc attccacatc cggacgtcgg
    13681 acgttggcgg caacatgttc ttggggttag tgtcgagctg aattcttggc agctgggcgg
    13741 caaaaaccag agtaagcagc aacgcaaccc cgaagatgac cttcgggtgg gcaatggaaa
    13801 attctacgag ggtgttcttc tgcatcagca gtcaaaaacg gctgtaaagc cagaacttct
    13861 cgtaaaacac cttgccgagg tgcatcatga tgctgggcgg tttcagcgta atttgcggcg
    13921 caggctcggc gtagaaattc ccgctgccga agccggcgcg gccgtcacct gcctcgagaa
    13981 agcattcgcc atcgccgttg aaccgctgcg tctcgccctt gccggtgatg gcgtggacga
    14041 tgttattggc gacgacctcg gcctcgtagt gggcgaaaac cccggccttg ggcagcggct
    14101 tgccgatcga ggtgagcatg atcccggcga catcgccgat cgcgaatacg tcgggaaatt
    14161 tcgtcgccat ggtttcgcgg tcgaccggga cccagccgct ttcaccacac attcccgcgt
    14221 cgcgtaccac cttgggcgcg cgatgcggcg gaatgtacgc gagcaggtcg aattcagcgc
    14281 tggcgccgtt ggcgaacgtg atccggcgta gcgccgcatc cacgccggtc acggaatgat
    14341 tcggatggta gccgatgccc ttgctttcga tcatctgacg cagcgcggat gaaacagccg
    14401 ccccggcgac cggcatcggg cccggttccg ccgtgtaaag atcgatcttg acctcgtttc
    14461 ggataccgcg ccgccggcag tcgtattcca gcaacatcgc ggcctcgttc ggcgcgcccg
    14521 ggcatttgaa cggcatcgcg ctcaccagca cgacgacccg gcctttgctg agcttgtgcc
    14581 tggcatcccg cagcgattcc gctccggcca gcgtgtagaa attgtggcct gctgcagaaa
    14641 gccccggcac cgattccgga gccagttctg cgccgagcgc aacgatcatg taatcgccgg
    14701 ttagctcacg ggcattgacg aataccgcgc gccgcgccgg atcgatcttt tcgatattgc
    14761 ctcgtacgac ttcgattccg cgtttggcga gagcggcga
//
LOCUS       DBNGWZ010000649         6482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3387654, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000649 DBNGWZ010000000
VERSION     DBNGWZ010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3387654"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1031
                     /locus_tag="AC16CX_20335"
     CDS             258..1031
                     /locus_tag="AC16CX_20335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3014888.1"
                     /translation="MGQLTGKVAVVTGASTEKGIGSAIARRYAQEGASVFLVAEGTRE
                     QLEKTQQECRGYPDAGRVEYGIFDLSQRGVAEKMIEEAAGRFGRVDILVNNAGIRAAH
                     DFGEYTRDVFDRVIAVNLAAPFFASQAVLPIMRKQGGGRIINIASQLARVTYAKRALY
                     GLTKAALVHLTKSMAYELARAGIMVNSISPGPVRTQFVIDRDAADPEAAQRRLKTYVP
                     VGRLGEPWEIAEVALFLASTPATFLQGEDICIDGGYTAH"
     gene            complement(1059..2033)
                     /locus_tag="AC16CX_20340"
     CDS             complement(1059..2033)
                     /locus_tag="AC16CX_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014889.1"
                     /translation="MNRFLIIFACRAVALLAAFVPVLAAAQDYPQKSIRFIVPFPPGG
                     ATDGLARILGEKMTDAWKQQVVIDNRGGAGGNIGAEIAAKSPPDGHTIIVVGLSHAAN
                     LSLYSRLAYHPVRDFAPVTQAVAIHTFLVVHPSVPVKSVKELLALARAKPGSLNYASG
                     GNGSSPHMAMELFKSLAKVEFVHVPYKGTESVIGLLRGEAALLFENLISVGAHVKSGK
                     MRVLAIGASKRWSGMPNVPTVAEAGVPGYDMVLWFGVLAPAGTPKPVVARLHREMAGI
                     LKLPDVRERLAGQGADPIGSTPEEFDAFIKAEIAKWAKVVKAAGLKVE"
     gene            complement(2121..3152)
                     /locus_tag="AC16CX_20345"
     CDS             complement(2121..3152)
                     /locus_tag="AC16CX_20345"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014890.1"
                     /translation="MTHALNARPGARSSLVARRERLPGTVAALALFLAVCPAEAADRP
                     TYPSKPIRVIVGFAPGGSADITARTVGQKLSELLGQPVVVDNRSGASGIIGGELAARA
                     APDGYTLLEATMTTHGIGPNLYQKLPYDPIKSFEPIALMVSIPLVMFVHTSVPGTSLK
                     EVVTLLKASPGKYRYATAGNGSPPHLAAELFKLKAGVDLLHVPYKGTGAAVPDVVGGQ
                     VHFMIDGPPPFLGYVKAGRVRALAAANAKRLAQLPDVPTFAESGYSGMEAGLWYGMLA
                     PRGTPRDIVNRLNAAINKVLQQPDVRERFAASSVEIVGGTPQEFGNYIAGEIRRWGEV
                     ARAAKIRIE"
     gene            complement(3221..4111)
                     /locus_tag="AC16CX_20350"
     CDS             complement(3221..4111)
                     /locus_tag="AC16CX_20350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006163788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3014891.1"
                     /translation="MLKTHGRYDYSPISKRADYSWPGGRRLALHFSLNVEHFEFGQGL
                     GNDYAAPHPPPNTRSYAWRDFGNRVGAWRLLELAERYDLPYALLVNTELYDYCPELIA
                     AFREHGDEIVGHGRTDAERQADMNFEQERACILEATSLIEKHEGKKPAGWLAPYISQT
                     YDSLDLLKEAGYRYMMDWPLDDQPVWFRTRHGPILSIPYAHDLNDSLECVTRRTPAQL
                     HCGNLIDQFDAMLEESARRPLVMSIVLHSFVLGQAYRARQFSRVVEHILRHRDRIWIT
                     RPGDICAHIESLPKGIVPGS"
     gene            complement(4130..5560)
                     /locus_tag="AC16CX_20355"
     CDS             complement(4130..5560)
                     /locus_tag="AC16CX_20355"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014892.1"
                     /translation="MAWNSSRRLTRYNERAEIDCQMPSRIPPPYLETISRFIHGVTLP
                     DVPAEAKDRALWVIADCVPVIAAGMQQPEMKAFVAKHLANAASGNAWVVGAGRRAAAL
                     DASLLNGTAGTWLELDEGNLFAKGHPGIQVVPAAIALAQEARVSGADLLLAVALGYEI
                     SARISRAAQMRLAVHPHGTYGVIGAAIAAARLKGFDARQARELLNIAATMGMATSRQT
                     LLDGATVRNIFTGHSGYMGITAARLVECGFTGEIDGVGNVYGKVLSDTFKREEVTSGL
                     GTDWLITRNYFKLHPTGRYVHSAIDALENLLRKVPGGRLDVDRIKEIRVRTYMLGAML
                     AEKNAVSSFGARFSLPFALASILYHGRSGLASFDEHAVANPKIREIANLVDLREDEQH
                     TARYPEEQPCDLRITLTDGAVWEGHCTVMKGEPANPHRPEELEAKFFDLGAPVWGEAV
                     TKRLFEGLRNLENIPDFGAFADQFSL"
     gene            complement(5589..>6482)
                     /locus_tag="AC16CX_20360"
     CDS             complement(5589..>6482)
                     /locus_tag="AC16CX_20360"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014893.1"
                     /translation="YPAKPVRLIVAQGTGSSVDNMGRVLAMRLTEEWGKQVIVDNRGG
                     AGGTIGAEIASRAAPDGYTLLLSSTSMQVISPQIYKNLTYHPTKDFKQISLIANTQNV
                     LVVNSNMPFKTVKELIAYARANPGKLNMANAGSGFQSHLAAVLFAHMADIDVHHVPYK
                     GGTSTTMVAAGESHLTIVPLPSVIAHLRAGRLRPVGAGGEKRSPQLPDVPTISESGVP
                     GYVSTGWAGIVVPKGASRPLFDRIYASLVKVMNDPTTRELFERQGAEPMTSTPEQMLN
                     HINAEYGRFAQAIKLAGLKMQ"
BASE COUNT         1131 a         2069 c         2083 g         1199 t
ORIGIN      
        1 aggtcaaagt ctgccgcacg ggcgatgcag tcgcggagct caatcgcctg cagcgcgagg
       61 ccgcgcgcgt cgtggccgcg ttgcacctca cttgctgagc gcgcggcgga cggaccggaa
      121 ccggatctga cccgcgcaac gcatggaatc ggcgatctag gaacgcaatg tagtggtgcc
      181 ccgcgcgctt agaggcgctt tcttggggta aaagggacag cttgctcggg ccccgttttt
      241 cgcatggaag gaacgacatg ggacagttga ccggaaaagt ggccgtagtc accggcgcct
      301 ccaccgagaa gggcatcggc agcgcaatcg cgcggcgcta cgcgcaggag ggtgcgtcgg
      361 tgtttcttgt ggcggaaggc acccgggagc agctcgagaa aacacagcag gagtgccggg
      421 gctatccgga tgcgggacgg gtggagtacg ggatctttga cctctcgcag cgcggtgtgg
      481 cggagaagat gatcgaggaa gcggccgggc gcttcgggcg cgtggacatc ctcgtcaaca
      541 acgccgggat acgcgccgcg cacgacttcg gcgagtacac ccgcgacgtg ttcgacaggg
      601 tgatcgcggt caatctcgcc gcgccgttct tcgcgagcca ggcggtgttg cccatcatgc
      661 gcaagcaggg cggcggccgc atcatcaaca tcgcgagcca gctggcgcgt gtcacgtacg
      721 ccaagcgcgc cctctacggc cttaccaagg cagcgctggt ccacctcacc aagtcgatgg
      781 cctacgagct cgcgcgcgcc gggatcatgg tgaattccat cagccccggg ccggtcagga
      841 cgcagttcgt catcgatcgt gacgcggcgg atccggaggc ggcgcagcgt cgcctgaaga
      901 cctacgttcc cgtcggccga ctgggcgagc cgtgggagat cgccgaggtc gcgctctttc
      961 tcgcctcgac gcccgcaact tttctgcagg gtgaggacat ctgcatcgac ggaggctaca
     1021 ccgcgcacta gcgcgccgat ccccgggctg cgcgagccct actcgacctt gagaccggct
     1081 gctttcacca ctttcgccca cttggcgatc tcggccttga tgaaagcgtc aaactcctcg
     1141 ggcgtgctgc cgatcgggtc ggcgccctgg ccggcgagcc gctccctcac gtcgggcagc
     1201 ttcagtatgc cggccatctc ccgatggagc ctggcgacga cgggcttcgg cgttccggcg
     1261 ggggcaagca ccccgaacca cagcaccatg tcgtagccgg ggactccggc ctccgccacc
     1321 gtcgggacat ttggcatccc gctccagcgc tttgatgcgc cgatggcgag cacccgcatc
     1381 ttgccggatt tgacatgggc accgactgaa ataagattct cgaacagcag cgccgcctcc
     1441 ccgcgcagca gcccgatcac cgattccgtg cccttgtacg gcacgtgcac gaattcgacc
     1501 ttcgccaggc tcttgaaaag ctccatcgcc atgtgcgggg agctgccatt gccgcccgag
     1561 gcgtagttga gcgacccggg cttcgctctc gcgagcgcca gcagctcctt caccgacttc
     1621 accggcaccg aggggtgcac cacgaggaag gtgtggatcg cgacggcctg cgtgaccggc
     1681 gcaaagtccc tcaccggatg gtaggcgagc ctggaataga ggctcagatt cgccgcgtgc
     1741 gacagcccga cgacgatgat ggtgtggccg tctggcggcg acttcgcggc gatctccgcg
     1801 ccgatgttgc cgcccgcgcc accgcgattg tcgatcacca cctgctgctt ccacgcgtcg
     1861 gtcattttct cgccgaggat ccgcgcgagc ccgtcggtcg cgccgccggg cgggaacggc
     1921 acgatgaagc ggatggactt ctgcgggtag tcctgcgcgg ccgcgagtac cggaacaaac
     1981 gcggcgagga gggcgacggc acggcaagca aagatgatca agaacctgtt catggagctt
     2041 tcctccccgt taatcagatg cgaatacgag ccgcgtcatt attacgctcg cagtagagag
     2101 cgggaacagc tccccaaaga ctattcgatg cggatcttgg ccgcacgggc gacctcgccc
     2161 cagcgcctga tctcgccggc gatgtaattg ccaaattcct gcggtgtccc gccgacgatc
     2221 tcgactgaag aggccgcgaa gcgctcgcgc acgtccggct gctgcagcac cttgttgatt
     2281 gccgcgttca ggcggttgac gatgtcccgc ggcgtgcccc tgggcgcgag catgccgtac
     2341 cacaggcccg cttccatgcc gctgtagccg gactcggcga acgtgggcac gtcgggcaac
     2401 tgtgccagcc gcttcgcatt cgcggcggcg agcgcccgca ccctgcctgc ctttacgtac
     2461 ccgagaaatg gcggggggcc gtcgatcatg aaatggactt gtccgccgac cacgtccggc
     2521 acggccgcgc ccgtgccttt ataaggcacg tgcagcagat ccacgcccgc cttcagcttg
     2581 aacagctcgg cggcgagatg tggcgggctg ccgttgccgg cggtggcgta gcggtacttg
     2641 cccgggctgg ccttgagcaa cgtaacgacc tccttgaggc tggttccggg caccgacgtg
     2701 tggacgaaca tgaccagcgg gatgctcacc atcaatgcga tcggctcgaa gctcttgatg
     2761 gggtcgtacg gcagcttctg atagagattg ggcccgatac cgtgcgtcgt catggtcgct
     2821 tccagcagcg tatagccgtc gggcgctgcc cgtgctgcca gctcgccgcc gatgatgccc
     2881 gaggctccgc tgcggttgtc cactacgacc ggctgtccga gcagttcgga aagtttctgt
     2941 ccgacggtgc gcgcggtgat gtcggcggag ccacccggcg caaatcccac gatcacacgg
     3001 atcggcttgg acgggtaggt cgggcgatca gctgcctctg ccgggcacac tgcaaggaac
     3061 agagcaagtg cggcaacggt gcccggcagc cgctcgcggc gcgccacgag ggatgatcgg
     3121 gcgccaggac gcgcattcaa agcgtgggtc acgggttttt ctcctcggat tttgattcgg
     3181 agttgggtga tacgaccgga aaattatagc ttgcgccggc tcaactgccg ggcacgattc
     3241 ccttcggcag ggattcgatg tgcgcacaaa tgtcgccggg ccgtgtaatc cagatcctgt
     3301 cgcggtgccg cagaatgtgc tcgacgacac ggctgaactg gcgcgcccgg taggcctggc
     3361 ccagcacaaa gctgtgcaag acaatggaca tcacgagcgg gcgccgcgcc gactcttcca
     3421 gcatcgcatc gaactgatca atgagattcc cgcagtgcaa ttgcgcgggc gtgcggcgcg
     3481 taacgcattc gagcgaatcg ttgagatcgt gcgcataggg gatggacaga atggggccgt
     3541 gcctggttct gaaccatacg ggttggtcgt ccagcggcca atccatcata tagcgatatc
     3601 ccgcctcctt cagcagatcg agggaatcat atgtctgtga gatgtaaggc gccagccacc
     3661 cggccggttt cttgccctcg tgtttctcga tcagggaggt cgcttcaagg atgcacgcgc
     3721 gctcctgctc gaaattcata tcggcctggc gttcggcgtc ggtccggccg tggcctacga
     3781 tttcgtcgcc gtgctcgcgg aatgccgcga tcagttccgg gcagtagtca taaagctcgg
     3841 tattgacgag gagcgcgtag ggaagatcgt aacgctcggc gagctcgagc aagcgccacg
     3901 caccgactcg attcccgaaa tcgcgccacg cgtaactgcg tgtgttgggc ggcggatgcg
     3961 gcgcggcata atcgttgccc agtccctggc cgaactcgaa gtgctcgaca ttgaggctga
     4021 aatggagcgc caggcgcctg cccccgggcc agctgtagtc ggcgcgcttg ctgatcggtg
     4081 aataatcgta acgtccatgc gtcttgagca tcgtgtggtc cccgtgtgcc taaagcgaaa
     4141 attgatcggc aaatgcgccg aaatcaggta tgttctccag attcctcaac ccctcgaaca
     4201 gccgcttcgt gacagcctcg ccccacaccg gcgcgcccag atcgaaaaat ttcgcctcga
     4261 gctcttccgg ccggtgagga ttggcgggct cccctttcat cacggtgcag tgcccttccc
     4321 ataccgcacc gtcggtgagt gtaatcctga gatcacaggg ttgttcctcg ggataccgcg
     4381 ccgtgtgctg ctcgtcttcc cgcaggtcga caaggttcgc gatttcccgt attttcggat
     4441 tggcgacggc gtgctcatca aaactcgcca atcccgagcg gccgtggtac aggatgctgg
     4501 ccagggcgaa gggcaatgaa aaacgcgcgc cgaagctcga gaccgcgttt ttttccgcga
     4561 gcatggcgcc cagcatgtag gtgcgcaccc tgatctcctt gatgcggtca acgtcaaggc
     4621 gcccgccggg aaccttcctg agcaggttct cgagcgcatc gattgccgaa tgcacatagc
     4681 ggccggtcgg gtgcaacttg aagtaattgc gcgtgatcag ccagtcggtg ccgagtcctg
     4741 aagtcacttc ctcgcgcttg aaggtatcgg acaggacctt cccatatacg ttgccgacgc
     4801 cgtcgatctc gccggtaaag ccgcactcga ccagccgcgc cgccgtgatc cccatgtacc
     4861 cggaatgtcc ggtgaaaatg ttacggactg tcgcgccgtc gagcagcgtc tgacggctgg
     4921 tggccatacc catggtcgcc gcgatattga gaagttcgcg cgcctgccgc gcatcgaagc
     4981 ctttcagccg cgccgcggct attgcagcgc cgatgactcc gtaagtgcca tgcggatgta
     5041 ccgcgagccg catctgtgcc gcgcggctga ttcgtgccga tatctcatag ccgagcgcga
     5101 ccgcgagcag cagatctgcg cctgaaaccc ttgcttcctg cgccaatgca attgctgccg
     5161 gtactacctg tatgccggga tgacccttgg cgaaaagatt gccctcgtcc agctcgagcc
     5221 aggtgccagc cgtaccattg agcagcgagg catcgagcgc ggcggcacgc ctgcccgctc
     5281 caaccaccca ggcgttgcct gaagctgcgt tggccaaatg ctttgcaacg aacgccttca
     5341 tctccggctg ttgcatgccg gcggcaatca ccggaacgca gtcggcaatc acccaaagtg
     5401 cgcgatcttt cgcctctgcc ggcacatccg gcaatgtaac gccatggata aatcgactaa
     5461 tcgtttcgag atagggcggc gggattcgag atggcatctg gcaatctatc tcggctcttt
     5521 cattatagcg agtcaggcgc cgtgaactgt tccatgccat tctcgggccg catagggcgg
     5581 ccggcgctct actgcatctt gaggccggcg agcttgatcg cctgggcgaa gcggccgtac
     5641 tccgcgttga tgtggttcag catctgctcc ggggtgctcg tcatcggctc cgcgccctgg
     5701 cgctcgaaca gctcgcgcgt ggtcggatcg ttcatgacct tgacgagcga ggcgtagatc
     5761 cggtcgaaga gcggcctgga ggcgcccttg ggtaccacga tgcccgccca gcccgtggat
     5821 acgtagcccg ggacgccgga ctcggaaatg gtcggcacgt ccggcagctg cggcgagcgc
     5881 ttctctccgc cggcacccac cgggcgcagg cgccccgccc gcaggtgcgc gatcaccgac
     5941 ggcagcggca cgatcgtcag gtgggactcg ccggcggcca ccatggtggt cgaggtgccg
     6001 cccttgtagg gaacgtggtg cacgtcgatg tcggccatgt gcgcgaacag cacggcggcg
     6061 agatggctct ggaagcccga tcccgcgttc gccatgttga gcttgcccgg gttcgctctt
     6121 gcgtaggcga tgagctcctt cacggtcttg aacggcatgt tggaattcac caccagcacg
     6181 ttctgcgtgt tggcgatcag cgagatctgc ttgaagtcct tggtcgggtg gtaggtgagg
     6241 ttcttgtaga tctgcggcga gatcacctgc atcgaggtcg atgacaggag cagcgtgtag
     6301 ccgtcgggcg cggcgcgcga ggcgatctcc gcgcctatcg tgccgccggc gccgccccgg
     6361 ttgtccacga tcacctgctt gccccattcc tccgtgagcc gcatggcgag gacgcggccc
     6421 atgttgtcga ccgagcttcc cgtcccctgc gcgacgatca ggcgcaccgg cttggcggga
     6481 ta
//
LOCUS       DBNGWZ010000650         4516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2631280, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000650 DBNGWZ010000000
VERSION     DBNGWZ010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2631280"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="AC16CX_20365"
     CDS             complement(<1..1066)
                     /locus_tag="AC16CX_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR3014894.1"
                     /translation="MKTTSKWYLGTAAAGMAAILSACAAQIGSQPVADPAIHIGDHDL
                     GGVVTSANGPEAGVWVIAETTNLPTKFAKIVVTDDRGRYVMPDLPKASYSVWVRGYGL
                     VDSPKVRTEPGRLLNLTAVIAPGAAAAAEYYPAIYWYSMLKIPAKNEFPLGPMKSQSE
                     WLNQIKTTGCMSCHALGTKGMRTMPKDFAHMKPTEAWVRRISSGQAMTQMINVLNRFD
                     RERVLAEWADWTDRVAAGELPFDRPSRPQGIERNIVLTLWDWSTPTGYMHDLISTDRR
                     KPTVNANGKLYGATEESTDFFPVLDPNTHTAGQIKHPVRDPKTPSSKSAPMAPSPYWG
                     PNPIWDSQTSIHNQMMDEKGR"
     gene            complement(1104..3254)
                     /locus_tag="AC16CX_20370"
     CDS             complement(1104..3254)
                     /locus_tag="AC16CX_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014895.1"
                     /translation="MKTKKAWYAGAAFGFAALLVACAAQQYGPPGSAEGVNIGENDLG
                     GVVIGPKGPEAGVWVIAETTDLPTRYIKSVVTDDQGRYVIPDLPLANYQVFVRGYGLV
                     DSPRVRAKPGQIVNHTAVPAPNERAAAQYYPAIYWYTMMKIPPAKDFGGSTAIPKNIT
                     QEIWRQRMNNVDCVGCHQLGQESTRTIPAQLGDYKSHEEAWMRRIASGQSGEMMVNRI
                     AGQLAGVPYKYFADWTARIEKGELPKNKPQRPQGIERNLVVTAWEWSTPGKYLHDLIS
                     SDRRKPTVNAYGKLYGSPEYSTDVMPILDPKTHQVTYFKMPVADPNMPVSLGPGHAGA
                     VKPTQPSPYWGDAILWDTRANNHNAMFDDKGRLWIAATVRGMDNPAFCKKGSDHPSAK
                     VFPLERSPRQVAILDPKTMKYSFIDTCFGTHHPQFGYDADNTLWLSGTGPVAGWVNTK
                     VWDETGNAARAQGWFPFILDTNGNGKVDQWTEPGKPAEAGKDMRIAGSGPYAVMPHPT
                     DGSVWYAFNVFAGPPGFLRFDPKTRLSEFYAIPKEGIGVRGGDIDKNGVLWGSGSNGT
                     LISFDRRKCKTPLNGPNATGNHCPEGFAYHKYPGPGFEGFENTSAEASYYTWVDHHNT
                     VGLGENVPISTANLQDGFVGYKDGQMILMRVPYPMGFYAKGMDGRIDDPNAGWKGRGL
                     WSASGDRTPWLNEFGKGARPLAVHIQVRPDPLAR"
     gene            complement(3363..4502)
                     /locus_tag="AC16CX_20375"
     CDS             complement(3363..4502)
                     /locus_tag="AC16CX_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3014896.1"
                     /translation="MPSIQSVSVCVVRVPLEHVTSFATRTVSARDYCLVKVRSTEGVE
                     GIGFCYVGSTAGNICKVAVEELLAKKLIGQESLRVEGLWQEMYQESLLQGRSGSVMRG
                     ISILDTALWDLNARAAKLPLHLYLGANVIDKVPAYASGGYYLAGKTPKKLGQEMAGYV
                     KAGFKAVKMKVGRLSPKEEEARVRAAREAIGPDIVLTLDANNAWSDVPTALQYVRRWE
                     QYDPYWIEEPFGPDDIDSHARLARLTPIVVATGEIAYGRWYHKELLDKRGAEILQTDA
                     CVCGGITEWRRIAAMAAGYGVTLCPHWFHDVHAPLVAATPNARYVEFFPDDQVLNFRR
                     LIDKQLEHKNGDLILHQTPGLGLRFDEKAVRKYALEPARPWTVIS"
BASE COUNT          705 a         1460 c         1436 g          915 t
ORIGIN      
        1 cccggccctt ctcgtccatc atctggttgt ggatgctggt ctggctgtcc cagatcgggt
       61 tcggccccca atagggcgac ggcgccatcg gcgcgctctt cgaggacggc gtcttcgggt
      121 cgcgcacggg gtgctttatc tgtcctgctg tatgcgtgtt cggatcgagc acggggaaga
      181 aatccgtgct ctcctccgtc gcaccgtaaa gcttgccgtt ggcgttgacc gtgggcttgc
      241 gccggtcagt cgaaatgaga tcgtgcatgt aaccggtggg cgtgctccag tcccacagcg
      301 tcaacacgat gttgcgctcg ataccctggg ggcgcgacgg ccggtcaaac ggcagctcgc
      361 cggcggcgac gcgatcggtc cagtcggccc attccgcgag cacccgttca cggtcgaagc
      421 ggttgaggac gttgatcatc tgggtcatgg cctggccgga ggaaatacgc cgcacccacg
      481 cctcggtggg tttcatgtgc gcgaaatcct ttggcatcgt gcgcatgccc ttggtgccca
      541 gcgcgtggca cgacatgcag ccagtggtct tgatctggtt cagccactcg gactggcttt
      601 tcatcggccc gagggggaat tcgttcttcg cggggatctt gagcatcgaa taccagtaga
      661 tcgccggata atattccgcc gccgcggccg caccgggcgc gatgaccgcg gtcaggttga
      721 gtagccggcc cggctcggtc cggaccttgg gcgaatccac caggccgtag ccgcgcaccc
      781 agacgctgta actcgccttc ggcagatcgg gcatgacgta gcgcccgcga tcgtcggtga
      841 ccacgatctt ggcgaacttg gtgggaagat tggtcgtctc cgcgatcacc cagacgccag
      901 cctccggtcc gttcgcgctc gtaacgacgc cgccgaggtc gtgatcgccg atgtggattg
      961 ccgggtcggc aaccggctgc gagccaatct gcgccgcgca cgcggacaga atcgcggcca
     1021 tgcccgctgc tgcggtgccc aggtaccatt tgctcgtggt tttcattttg ctgctcctcc
     1081 agaacaacgc aattgcctca gcctcagcgc gcgagcggat cggggcgaac ctggatgtgc
     1141 actgcgagcg gacgcgcgcc cttgccgaac tcgttgagcc agggcgtgcg atctccgctg
     1201 gcgctccaca ggccccggcc cttccagccg gcattcggat cgtcgatgcg cccgtccatg
     1261 ccctttgcgt agaaccccat cgggtacggc acgcgcatca ggatcatctg cccgtccttg
     1321 taacctacga agccgtcctg cagattggcg gtcgagatcg gaacgttctc gccgaggccg
     1381 accgtgttgt ggtggtcgac ccaggtgtag tagctcgctt ccgcgctcgt gttttcgaag
     1441 ccctcgaatc ccggacctgg atacttgtga tatgcgaagc cctcggggca gtgattgccg
     1501 gtggcgttag gtccgttgag cggggtcttg cacttgcgcc gatcgaagct gattagcgtg
     1561 ccgttcgagc ccgagcccca gagcacgccg ttcttgtcga tgtcgccgcc gcgtacgcca
     1621 atgccttcct tcggaatggc gtagaactcg ctgagccggg tcttgggatc gaagcgcagg
     1681 aatccggggg gaccggcgaa cacgttgaag gcgtaccaga ccgagccgtc ggtcgggtgc
     1741 ggcatcacgg cgtagggacc ggatcccgcg atccgcatgt ccttgcccgc ttccgccggc
     1801 ttgcccggct cggtccattg gtcgaccttg ccgttgccgt tggtgtcgag gatgaacggg
     1861 aaccagccct gcgccctggc cgcatttccg gtttcatccc acaccttggt gttgacccac
     1921 ccggcgaccg gacctgtgcc cgagagccac agcgtgttgt ccgcgtcgta gccgaactgc
     1981 gggtgatggg tgccgaaaca ggtgtcgatg aaggagtact tcatcgtctt cggatcgagt
     2041 atcgcaacct ggcgcggcga gcgctcgagc ggaaacactt tggcggacgg gtgatccgag
     2101 cccttcttgc agaaggctgg attgtccatg ccgcgcacgg tcgctgcgat ccacagccgg
     2161 cccttgtcgt cgaacatagc gttgtggttg ttggccctgg tgtcccacag gatcgcgtca
     2221 ccccagtagg gcgacggttg cgtcggcttg accgcgccgg cgtggcccgg tccgagcgaa
     2281 accggcatgt tcggatcggc cactggcatc ttgaaatacg tcacctggtg cgtcttggga
     2341 tcgaggatcg gcatcacgtc ggtactgtat tcgggtgagc cgtagagctt gccgtaggcg
     2401 ttcaccgtcg gcttgcgccg gtccgaggaa atcaggtcgt gcaggtactt gccgggggtg
     2461 gaccattccc acgccgtgac gacgagattg cgctcgatgc cctgcggccg ctgcggcttg
     2521 ttcttcggca actcgccctt ctcgatccgc gcggtccagt cggcgaaata cttgtatggc
     2581 acgccggcga gctgcccggc gatccggttg accatcatct cgccggactg gcccgaggcg
     2641 atacggcgca tccaggcctc ttcgtgcgac ttgtaatcgc cgagttgcgc tggaatcgtg
     2701 cgcgtcgatt cctgcccgag ctgatggcag ccgacgcagt cgacgttgtt catgcgttgg
     2761 cgccaaatct cctgggtgat gttcttcgga atcgcggtcg aaccgccgaa gtccttggcc
     2821 ggcggaatct tcatcatggt gtaccagtag attgccggat aatactgcgc cgccgcccgc
     2881 tcattcggcg ccggcaccgc ggtgtggttg acgatttggc cgggtttcgc gcgcaccctc
     2941 ggcgaatcca cgagaccgta gccgcgcacg aagacctgat aatttgcgag cggcaggtcg
     3001 gggatgacgt aacggccctg gtcatcggtg acgacactct tgatgtagcg ggtcggcaga
     3061 tcggtcgtct cggcgattac ccacacgccc gcttcgggcc ctttcgggcc gatgaccacc
     3121 ccgccgagat cgttttcacc gatgtttacg ccttcagcgc tacctggcgg gccgtattgt
     3181 tgagccgcgc acgcgacgag cagcgcggcg aaaccaaacg cggcgcccgc ataccatgct
     3241 tttttcgtct tcatttgctc ctccttcggg tctgagtgct gattcgagca gtcatttccc
     3301 gccgaataaa gtctagccag tccccgattt tccgtcaccc ttatctcgtc tcccttgcct
     3361 cgtcacgaga tgacggtcca gggcctcgca ggttcaagcg cgtacttcct caccgctttc
     3421 tcgtcgaacc gtaagcccag cccgggcgtc tggtgcagga tcaggtcgcc gttcttgtgc
     3481 tcaagctgct tgtcgatcaa gcgccggaag ttgagcacct ggtcgtcggg gaagaactcg
     3541 acgtagcgcg cgttgggcgt cgcggccacc agcggtgcgt gcacgtcgtg gaaccagtgc
     3601 ggacagaggg tgacgccgta gcccgccgcc atcgccgcga tgcgccgcca ttccgtgatg
     3661 ccgccgcaca cgcaggcgtc ggtctgcaag atctccgctc cgcgcttgtc gagcagctcc
     3721 ttgtggtacc agcgcccgta ggcgatctcg ccggtcgcca ccacgatcgg cgtaagccgc
     3781 gcaagcctgg cgtggctgtc gatgtcgtcg ggcccgaacg gctcctcgat ccagtacggg
     3841 tcgtactgct cccagcgccg gacgtattgc agcgccgtcg gcacgtcgct ccaggcattg
     3901 ttcgcgtcca gcgtcagcac gatgtcggga ccgatcgcct cgcgcgccgc gcgcacgcgc
     3961 gcctcctcct ccttcggcga cagccggccc actttcatct tgaccgcttt gaagcccgcc
     4021 ttcacgtagc ctgccatctc ctggcccagc tttttcgggg tcttgccggc gaggtagtag
     4081 ccgccgctcg cgtacgccgg aaccttgtcg atgacattgg cgccgagata gaggtgcaga
     4141 gggagcttcg ccgcgcgcgc gttgaggtcc cacagcgcgg tatcgaggat ggaaatgccg
     4201 cgcatcacgc tgcccgagcg gccctgcagg agcgactcct gatacatctc ttgccacaac
     4261 ccctcgacgc gcagcgattc ctggccgatg agcttcttcg cgagcaactc ctcgaccgcg
     4321 accttgcaga tattgccggc ggtgctgccg acgtagcaga agccgatgcc ttccacgccc
     4381 tcagttgagc gcaccttcac caggcagtag tcgcgcgcgg agaccgtgcg cgtggcgaag
     4441 gaggtgacat gttcgagcgg cacgcgcacg acgcagacgg aaacggactg gatgctgggc
     4501 atggtcttga tttcct
//
LOCUS       DBNGWZ010000651         1778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_115147, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000651 DBNGWZ010000000
VERSION     DBNGWZ010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_115147"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16CX_20380"
     CDS             complement(<1..307)
                     /locus_tag="AC16CX_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014897.1"
                     /translation="MNQADLQALRTPLIVLLVVLLAAAAAIYYSGRLTAAALQQLSQQ
                     QAQLREARIRLQKSGDEKDIIVRYLDAFRQLERAGFVGEEQRINWLDGLRVTNQQADL
                     "
     gene            complement(304..>1778)
                     /locus_tag="AC16CX_20385"
     CDS             complement(304..>1778)
                     /locus_tag="AC16CX_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014898.1"
                     /translation="FKEFLAPFIQVPVHIMVDAVEEDYRFETLPHASGSDRADMVGRK
                     LKQHYRNTPFMAASLLGRDIGKRRDDRYLFSALTNPEVVNDWLQAVAARGLPVAGIYL
                     LPMVSAGLLERLDAKAANLLLVAQHANGLRLTFFHERQFRLSRLTRGDGGRAENRVRY
                     FAEEISNTRLYLHALRTMTLDEQLMVLLVDRGDELAEVAAGVARENPSFECVRLGRQE
                     LVGRLGIADSVLAASPYAIYLHLLGLKEPPSNLAPASVTVGYKRHQARRGIYAACGAL
                     ATGAVIWTGANFYQTMMLRGETEDAVRQTALLSAQYQQVTRQFPQAPASAENLKKTVE
                     IAQKLRETAHTPQRLLALVSRALEASPTIVVKEFGWKYGTSEIEIQGPGTRSTTESAA
                     AAPATPTSAKQAVARRESALIEGEIRPFRGDYRSAIATINGLAGRLAKAPEVSEVRVV
                     KLPLDVNPTLSLSGNTLENPEQSATGTAVFKLLLVLKPNA"
BASE COUNT          271 a          589 c          609 g          309 t
ORIGIN      
        1 acagatcggc ttgctggttg gtcacgcgca gcccgtcgag ccagttgatg cgctgctcct
       61 cgccgacaaa gcccgcgcgc tccagctgcc ggaaagcgtc gaggtaacgc acgatgatgt
      121 ccttctcgtc gcctgatttc tgtaaccgga ttctcgcctc cctgagctgg gcctgctgct
      181 gggaaagctg ctggagcgcc gccgcagtca accggcctga ataatagatg gccgcggcgg
      241 cggcgagcag caccaccagg aggacgatca gcggggttct cagcgcctgg aggtcggcct
      301 ggttcatgcg ttgggcttga gcaccagcag cagcttgaac accgccgtgc cggttgcgct
      361 ctgctcgggg ttctcgagcg tgttgccgga gagggacagc gtgggattga cgtcgagcgg
      421 cagtttcacc acccgcactt cggacacctc cggcgccttg gcgaggcggc cggcaagacc
      481 gttgatggtg gcgatcgcgc tgcggtagtc gccgcggaac ggccggattt cgccctcgat
      541 cagcgcgctc tcccggcgcg ccaccgcctg cttggcgctg gtgggcgtgg ccggcgccgc
      601 tgccgcagat tcagtggtgg agcgtgttcc cggcccctgg atttcgattt cgcttgtgcc
      661 gtacttccac ccgaattcct tgacgacgat ggtggggctc gcctccagcg cccggctgac
      721 gagggccagc agccgctgcg gagtatgcgc cgtttcgcgc agcttctgcg cgatctcaac
      781 cgtcttcttc aggttctccg cggaagccgg ggcctgcgga aactggcgcg tgacctgctg
      841 atactgggcg gacagcaaag ccgtctggcg caccgcgtcc tcggtttctc cgcgcagcat
      901 catggtttga tagaagttgg cgccggtcca gatcacggcg ccggtcgcga gcgcgccaca
      961 agcggcgtaa atgccccgcc gggcctggtg gcgcttgtag cccactgtaa cgctcgccgg
     1021 cgcgagattg ctcggcggct ccttgaggcc gagcaggtgc agatagatcg cgtacggcga
     1081 ggcggcgagc accgaatcgg cgatgcccaa acgcccgacc agctcctggc ggccgaggcg
     1141 cacgcactcg aagctcgggt tttcgcgcgc gacgccggcg gccacctcgg ccagctcgtc
     1201 gccgcggtcc acgagcagca ccatgagctg ctcgtcgagc gtcatggtgc gcagcgcgtg
     1261 gagatagagg cgggtattgg agatttcctc cgcgaagtaa cgcacgcggt tctccgccct
     1321 gccgccgtcg ccgcgcgtga gccggctcag ccggaactgg cgctcgtgga agaacgtgag
     1381 ccgcaggccg ttggcgtgct gcgccaccag caacaggttg gccgccttgg cgtcgagccg
     1441 ttccagcaat cccgcgctca ccatcggcag caggtagatc cccgccaccg gcaggccacg
     1501 ggccgccacg gcctgcagcc agtcgttgac cacttcggga ttggtcagcg cggagaagag
     1561 atagcggtcg tcacgccgct tgccgatgtc gcgtcccagc agcgaggcgg ccatgaacgg
     1621 cgtgttgcgg taatgctgct tgagcttgcg gccgaccatg tcggcgcggt cgctccccga
     1681 cgcgtgcggc agggtctcga aacggtagtc ctcctcgacc gcgtccacca tgatgtggac
     1741 cggcacctgg atgaacgggg cgaggaactc cttgaagc
//
LOCUS       DBNGWZ010000652         7453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1875593, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000652 DBNGWZ010000000
VERSION     DBNGWZ010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1875593"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC16CX_20390"
     CDS             complement(<1..449)
                     /locus_tag="AC16CX_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014899.1"
                     /translation="MTKGSSAKTIGIVVVGLVVAAGLFYWGYSVHKQREQNKAAALRL
                     VSDTSARLRDALNVETAPPTTDRTRFVKKLDDHAATAERNLQVFRRIDLARHRALTDA
                     ADDYLLTAQEILKRQAESHRYRLLLAQSSQALLDHMRADNRTGAWVQ"
     gene            complement(519..2354)
                     /locus_tag="AC16CX_20395"
     CDS             complement(519..2354)
                     /locus_tag="AC16CX_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR3014900.1"
                     /translation="MSESTAARPKKAAPQLPSASNFIRAAIEADLASGKYAARGWGGR
                     PGPANVHAGAPQDPARIRTRFPPEPNGYLHIGHAKSICLNFGLARDYDGACHLRFDDT
                     NPEREEQEYVDSIVAAVKWLGFDWGPHLYYASDHFDFMYHAAECLIEEGHAYVDSLSA
                     EQMRALRGTLIEPGKNSPYRDRSADENLKLFRQMRDGRFAEGEHVLRAKIDMASPNIN
                     MRDPAIYRIRKVAHHRTGDKWCVYPMYTYAHPIEDALERITHSLCTLEFEDQRPFYEW
                     LLECLADSGLLARPLPQQIEFARLNLTYVVLSKRRLIRLVEEKYVDGWDDPRMPTLAG
                     ARRRGYTPEGIRLFAERIGVSKADSWIEYSMLEDCMREHLNEVAPRRIAVLDPVKLVI
                     DNYQAGKEEVCHLPNHPQHPALGTRAVPFSRELWIEREDVTEHPPKGYFRLFPGNEVR
                     LRYAFLVKCVDIEKDTAGNITTVHCTYDPETKSGTPGAEKRKVKGNVHWLSVKHAARG
                     EVRLYDRLFRAPYPGKKSSPPPHEEGQGEGGSGEGDWLADLNPDSVKIVTAQLEPALK
                     EARLEDCFQFERHGYFVADLKDSRPGIPVFNRAVTLRDSWAKNGK"
     gene            complement(2507..3469)
                     /locus_tag="AC16CX_20400"
     CDS             complement(2507..3469)
                     /locus_tag="AC16CX_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014901.1"
                     /translation="MNAVTLVLVALFSATLAVAPARAQSYPAKPIRIVVPFPPGGTSD
                     ILARLIGPKLTEKWGQSVVVESRPGAAGAIGHEVVAKSPPDGYTLVLSDVGPLVIVPV
                     LYPKTSYGLQDFAAITIISYSPHLLCVHPAVPVFTVPQLIALAKKQPGGLNYASSPAG
                     APYLAGLQFAYRTGIKWEYITGRGGAQSIMDVMTGQADVLFNGMLATLPPVKSGKLKL
                     LAVSSEKRHPSLPDTPAVAEFLPGFLTGSWQAVLAPARTPPDVVAALSTEVARVLFSP
                     EMKERLQAQGADPIGTSSADSMKFLRDERERFVKLVRETGYKPQ"
     gene            3516..4496
                     /locus_tag="AC16CX_20405"
     CDS             3516..4496
                     /locus_tag="AC16CX_20405"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR3014902.1"
                     /translation="MKALTSSVLKRLADGDFHSGEALARALDVSRASVWHAVRDVEAM
                     GLEVYKVRGRGYRLPQVITLLDRAAVEHHLGAHAGRFTIEIRDRVDSTNTALLERAAA
                     GARSGTMLAAEWQPGGRGRLGRTWHAGVGEALTFSLLWRFARGAGTLSGLSLAVGVAL
                     ARALRAAGAPDVALKWPNDVLWRGRKLSGILTELAGDALGPTAAVIGIGINVRLSEAT
                     LARIGQPATDLETACGAAPDRNQLLARLLTELDHVLEAFAREGFAPLRAEWQRAHAHP
                     GRRVALLLPDGTRHTGRARGVAEDGAFLLETRAGVRRYHSGEISLRPQKP"
     gene            4650..5384
                     /locus_tag="AC16CX_20410"
     CDS             4650..5384
                     /locus_tag="AC16CX_20410"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3014903.1"
                     /translation="MRILAIDAGNTRIKWGVHEDGAWRVQGWISTARPARLAREWAGL
                     ETPEAVIATNVAGERVARSIAAAVRRLGRRARFAKSVSSQCGVKNAYAEPAQLGPDRW
                     AALIGARHLHRGPCVVVNAGTTMTVDALTADGVFLGGMIVAGAELMREALARNTARLR
                     PRGGKFTFFPARTADAIESGAVNALAGAIERMRCFMRETGQDAPLTLLSGGAAHLIAP
                     RLNGAVEVVDNLVLEGLVRIALEREP"
     gene            5449..6150
                     /locus_tag="AC16CX_20415"
     CDS             5449..6150
                     /locus_tag="AC16CX_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR3014904.1"
                     /translation="MRAFFLLLVLANLVFLAYAHVAREEAGMQSRIPQLQVTPERIKL
                     LKAAGQAPPDKPKAPGKVIPPAPPKTASAVPAACLEWGVFAGPGVAKAEAALARLELP
                     PEKIERTVTDAGGYWVYIPPLKTKADADRKIGELKALGVTEFFVVQDPSQWRNAISLG
                     IFRNNEGAQAFLAKMKERGVRSAIAARRENFLKQVAFQVREPNEATVARLTALQQEFP
                     GSEIKTGPCPPASKG"
     gene            complement(6318..7352)
                     /locus_tag="AC16CX_20420"
     CDS             complement(6318..7352)
                     /locus_tag="AC16CX_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR3014905.1"
                     /translation="MPGRPRLLDQVHDAIRRRYFSRRTEEAYVHWIKRFIYFTGKRHP
                     LSVGEPEVTSFLNHLASERRVAASTQNQALSALLFLYKEVLGQELGWLDGLQRATRPP
                     RLPTVLTPAEAERLLAQLEGTRWLLASLLYGAGLRVIECLRLRVKDIDLSYRQILVRD
                     GKGEKDRVTMLPELTIEPLRAHLERVRLLHARDLREGYGEVHLPYALARKYPRAGREW
                     CWQYVFPSKNRSADPDDGVIRRHHLDEAVPQRAVKEAARAAGIPKHVNCHTLRHSFAT
                     HLLEGGYDIRTVQELLGHSDVSTTMIYTHVLNKGGRGVKSPLDSGGAGQPPAVRDVTA
                     EYRVEVSRVT"
BASE COUNT         1250 a         2410 c         2528 g         1265 t
ORIGIN      
        1 tcctggaccc aggcaccggt gcggttgtcg gcgcgcatgt gatcgagcaa cgcctgcgag
       61 ctttgtgcca gcaacaggcg gtagcggtgg ctctcggcct gcctcttcag gatttcctgg
      121 gcggtcagga ggtagtcgtc cgccgcgtcg gtcaatgccc ggtgccgcgc caggtcgata
      181 cgcctgaata cctgcaggtt gcgctccgcc gttgccgcgt gatcgtcgag cttcttgacg
      241 aaccgcgtgc ggtccgtggt gggcggcgcc gtttcgacgt tcaatgcatc gcgcagccgc
      301 gcgctcgtgt ccgagaccag cctcaaagcc gccgccttgt tctgctcgcg ctgcttgtgc
      361 acgctgtagc cccagtagaa gagccccgcg gccacgacga gcccgacgac gacgatacca
      421 atggtctttg cgctcgatcc cttagtcatt cgggcctgct cctcggcaag aaccttcata
      481 ttagggcagc gacacgccgg cagggaaggc catagttgtt atttgccgtt cttcgcccag
      541 gagtcgcgca gcgtcaccgc ccggttgaac acgggtatcc ccggtctcga atccttgagg
      601 tcagccacga agtacccatg ccgctcgaac tggaagcagt cctccagccg ggcctccttg
      661 agcgcgggct cgagctgagc ggtgacgatc ttcaccgagt cgggattcag gtcggcgagc
      721 cagtcccctt caccagaccc cccctcaccc tggccctcct catgaggggg aggggaggat
      781 tttttgccgg ggtatggcgc gcggaacagg cggtcgtaga gccgaacttc accgcgagcg
      841 gcgtgcttga cggacagcca atggacgttg cctttcacct tgcgcttttc cgctccgggc
      901 gtgcccgact tggtttcggg gtcgtaggtg cagtgcacgg tcgtgatatt acccgccgtg
      961 tccttctcga tgtccacgca cttcaccagg aacgcgtagc gcagccgcac ctcgttgccg
     1021 gggaagagac ggaagtagcc cttgggcgga tgttccgtca cgtcctcgcg ctcgatccag
     1081 agctccctcg agaacggcac tgcgcgtgtc ccaagcgctg ggtgttgcgg atggttgggc
     1141 agatgacaca cttcttcctt gccggcctga tagttgtcga tgacgagctt caccgggtcg
     1201 agcacggcga tccggcgcgg cgcgacctcg ttcaggtgct cgcgcatgca gtcctcgagc
     1261 atcgagtatt cgatccacga atccgccttc gacacgccga tgcgctcggc gaacaggcgg
     1321 atgccctcgg gtgtgtagcc gcggcggcgc gctccggcga gggtgggcat gcgcgggtcg
     1381 tcccagccat ccacgtattt ctcttcgacg agccgaatga gtctgcgctt ggagagcacg
     1441 acgtaggtga ggttgaggcg agcgaactcg atctgctgcg gcaacggccg cgcgagcagg
     1501 ccgctgtccg caaggcactc gagcaaccac tcgtagaatg ggcgctggtc ctcgaactcc
     1561 agcgtgcaca gcgagtgcgt gatccgctcc agcgcgtcct cgatcgggtg cgcgtaggtg
     1621 tacatcgggt agacgcacca cttgtcgccg gtgcggtgat gcgcgacctt cctgatgcgg
     1681 tagatcgcgg gatcgcgcat attgatgttg ggcgaggcca tgtcgatctt cgcgcgcaac
     1741 acgtgctcgc cctcggcaaa ccggccgtcg cgcatctggc ggaagagctt caggttctcg
     1801 tcggcgctgc ggtcgcggta cggactgttc ttgccgggct cgatgagcgt gccgcgaagc
     1861 gcccgcatct gctcggcgga caggctgtcc acgtaggcat ggccttcctc gatcaggcac
     1921 tcggccgcgt ggtacatgaa atcgaaatgg tcgctggcgt aatagagatg cggcccccaa
     1981 tcgaagccca gccacttcac tgcggcgacg atcgaatcca cgtactcctg ctcctcccgc
     2041 tccgggttgg tgtcgtcgaa gcgcaggtgg cacgcaccgt catagtcgcg cgcgagcccg
     2101 aagttgaggc agatggattt cgcgtggccg atgtgcaggt agccgttggg ctcgggcgga
     2161 aagcgcgtgc ggatcctcgc cgggtcctgc ggcgcgcccg catgcacgtt cgccgggccc
     2221 ggccggccgc cccagccgcg cgcggcgtat ttgccgctcg cgaggtcggc ctcgatcgcg
     2281 gcgcggatga aattcgacgc ggaaggcagc tgcggcgcgg cttttttcgg cctggcggcg
     2341 gtggactcgg acatgggcgc tattctagcc gagcaccgcg gcgggatcag ggacgaagga
     2401 aatgcgtgat aggtgatagg tgataggtga taagtgatag gtgtaaaagc cggcgctgcc
     2461 ggcgactctt atcacttatc actcatcact tatctcgcct tccaggctat tgcggcttgt
     2521 agccggtttc ccggacgagc ttcacgaagc gctcgcgctc gtcgcgcagg aatttcatcg
     2581 aatccgcgga agacgtgccg atcgggtctg cgccctgcgc ctggaggcgc tccttcattt
     2641 cgggcgagaa cagcacgcgc gccacctcgg tgctcagtgc ggcgacgaca tccggcggcg
     2701 tgcgcgcggg ggcgagcacg gcttgccagg aacccgtgag gaaacccggc aggaattcgg
     2761 cgacagccgg tgtgtcgggc aggctcggat ggcgcttctc gctggagacc gcgagcagct
     2821 tgagcttgcc cgacttgacg ggcggcagcg tcgcgagcat gccgttgaac agcacgtcgg
     2881 cctggccggt catcacgtcc atgatggatt gcgcaccgcc gcggccggta atgtattccc
     2941 acttgatgcc ggtgcgatag gcgaactgga gccccgcgag gtacggcgca cccgcgggac
     3001 tggacgcgta attgagcccg cccggctgtt tcttagcgag cgcgatcaat tgcggcaccg
     3061 tgaacaccgg cacggccggg tgcacgcaca ggaggtgcgg agagtaggag atgatggtga
     3121 tcgcggcgaa atcctgaagg ccgtagctcg tcttggggta gaggacgggg acgatcacca
     3181 gcggaccgac atcgctgaga accaaggtat agccgtcggg cggggacttc gcgactacct
     3241 cgtggccgat cgcgcccgcc gcgccgggtc ggctctcgac cacgaccgat tgcccccatt
     3301 tctccgtgag cttagggccg atcagccgcg ccaggatgtc ggaggtgccg cccggcggga
     3361 acgggacgac aatgcggatc ggcttggcgg gataagattg cgcgcgggcc ggtgcgacgg
     3421 cgagagtagc cgagaataac gcgacgagca cgagggttac ggcattcatc gcctggtttc
     3481 tcccgatatg cattgggctt attctatacc cgcacatgaa ggccctgacg tcgagtgtat
     3541 tgaagcggct tgcggacggt gatttccatt ccggagaagc gcttgcccgg gcgcttgatg
     3601 tctcgcgcgc gagcgtgtgg cacgcggtcc gggacgtgga ggcgatggga ctcgaggtct
     3661 acaaggtgcg cggccgcggc tatcgcctgc cgcaagtgat caccctgctc gatcgcgccg
     3721 ccgtcgaaca ccacttgggt gcgcacgccg ggcgcttcac gatcgagatc cgcgacaggg
     3781 tggactcgac caatacggca ctgctcgaac gcgctgcggc cggcgcgcgg agcggaacca
     3841 tgctcgccgc cgaatggcag ccgggcggac gcgggcgcct ggggcggacg tggcacgccg
     3901 gcgtgggcga ggccctgact ttttcgctgc tgtggcgctt cgcgcgcggc gccggcacgc
     3961 tttccgggct ctcgctcgcg gtcggcgtgg cgctcgcccg cgcgctgcgg gcagccggcg
     4021 cgccggacgt ggcgctaaag tggccgaacg atgtcctgtg gcgcggccgc aagctctccg
     4081 gcattctcac cgaactggcg ggcgatgcgc tgggaccgac cgcggcggtg atcggcatcg
     4141 gcatcaacgt gcgcctgtct gaagcgacgc tcgcgcgcat cgggcagcct gcaaccgacc
     4201 tcgagacggc atgcggcgcg gcgcccgacc gcaatcagct cctcgcgcgc ctgctgaccg
     4261 agctcgacca cgtgctggag gcgttcgcgc gcgagggctt cgcgcccttg cgcgccgaat
     4321 ggcagcgcgc ccacgcgcac ccgggcaggc gggttgcgct cttgctcccc gatggaacgc
     4381 gccacacggg acgcgcgcgc ggcgtggcgg aggacggcgc attcctgctc gagacgcgcg
     4441 cgggcgtgag gcgctatcac agcggggaaa tttccctgcg ccctcagaag ccgtgacaag
     4501 gctcgggaaa agagtgtcga ggattgagtg tcgaggggag aaacgaggga cgaggatgga
     4561 gggtcgaggg gggccctcgt cactcctcac tcgacccccg acactcgaca ctccgccatc
     4621 caccatcccg tacgcattac cattagcgca tgcgtatcct ggccatagac gctggaaaca
     4681 cccgcatcaa gtggggcgtg cacgaggacg gcgcctggcg ggtgcaaggc tggatctcca
     4741 ccgcgcggcc ggcgcggctc gcgcgcgagt gggccgggct cgagacgccg gaagcggtca
     4801 tcgcgacgaa tgtcgccggg gagcgcgtcg cgcgctcgat cgccgccgcc gtgcgccgcc
     4861 tcggccggcg cgcgcgcttc gcgaagagcg tgtcaagcca gtgcggcgtg aagaacgcct
     4921 acgccgagcc cgcgcagctc gggcccgacc gctgggcggc gctgatcggc gcccggcacc
     4981 tccaccgcgg cccgtgcgtg gtggtcaacg ccggcacgac gatgacggtg gacgcgctta
     5041 ccgcggacgg ggtgttcctc gggggaatga tcgtcgcggg agcggagctc atgcgcgagg
     5101 cgcttgcgcg caacaccgcc cgcctccgcc cgcggggggg caagttcacg ttctttccgg
     5161 cgcgcaccgc ggatgcgatc gaaagcggcg cggtcaacgc gctggcaggc gccatcgagc
     5221 gcatgcggtg cttcatgcgg gagacgggcc aggacgcgcc gctgacgctg ctctcgggcg
     5281 gcgccgcgca tctgatcgcg ccgcgcctta acggcgccgt cgaggtcgtg gataatcttg
     5341 tcctcgaagg gctcgtccgc atcgccctcg agcgtgagcc gtaagcagtg agcagtaagc
     5401 ggtaaattcc gctcgccgct caccgcttac cgcttaccaa gcgagcgcat gcgagcgttc
     5461 ttccttctgc tggtgctggc caacctggtg ttcctcgcct acgcgcacgt cgcccgcgag
     5521 gaggccggca tgcagagccg gatcccgcag ctgcaggtta ccccggagag aatcaagctg
     5581 ctcaaggcag cgggacaggc gccgcccgac aagccgaagg cgccgggcaa ggtgataccg
     5641 cctgcgccgc cgaagaccgc ttcggccgta ccagcggcct gtctcgagtg gggcgtcttc
     5701 gccggtcccg gcgtggccaa agcggaagcc gcgctggcgc ggctcgagct gccgccggag
     5761 aagatcgagc gcacggtgac cgatgccggc ggctactggg tctatattcc accgctcaag
     5821 accaaggccg acgccgaccg caaaatcggg gagttgaagg cgctcggtgt caccgagttc
     5881 ttcgtggtgc aggaccccag tcagtggcgc aacgcgatct cgctcggcat cttccgcaac
     5941 aacgaggggg cgcaggcgtt cctggcgaag atgaaggagc gcggcgtgcg ctcggcgata
     6001 gcggcgcggc gcgaaaactt cctgaagcag gtcgcgttcc aagtgcgaga gccgaatgag
     6061 gcgaccgtcg cacggctcac ggcgctgcag caggaatttc cgggcagcga gatcaagacc
     6121 gggccgtgcc cgcccgcgtc gaagggctag aacggcttac cgccaagaaa gatcaagatc
     6181 aatatttgaa ccgccaagac gccaagagaa acaagtcatc aatcgaaccg cagagacacg
     6241 gagagaacca gactcaggat tgaaccggcg agaattacgg gaacaagaat ctaaccgcat
     6301 gaatcacgac atccggctca agttactctg ctgacctcga ctctgtactc cgccgttacg
     6361 tcccgaacgg ccggcggctg gccggcaccg ccggaatcga gcgggctttt cacgccgcgc
     6421 ccgcccttgt tcagcacgtg ggtgtagatc atcgtggtgg acacgtcgga atggccgagc
     6481 aactcctgaa ccgtccgaat gtcatatccg ccctccaaca ggtgcgtcgc aaaggagtgg
     6541 cgcaacgtgt ggcagttcac gtgtttggga atacccgccg cgcgtgcggc ttccttgacc
     6601 gcacgctggg gcacggcttc gtcaaggtga tgtcgccgga tgaccccgtc atcggggtcg
     6661 gccgagcggt tcttcgaggg aaatacatac tgccagcacc actcgcgacc ggcgcgcgga
     6721 tatttccgcg caagggcgta aggcagatgc acttctccat agccctcgcg cagatcgcgc
     6781 gcgtgcaaca gacgcacccg ctccaggtgc gcgcgcagcg gctcgatcgt caactcgggc
     6841 agcatcgtga cccggtcctt ctcccccttg ccgtcccgaa ccaggatttg acgataggag
     6901 aggtcgatat ccttcacccg caaccgcagg cattcgatca cccgcagccc ggcgccatag
     6961 agcagactcg caagcagcca gcgcgtgcct tcgagctgcg cgagcagccg ctcggcttcg
     7021 gccggtgtaa gcacggtcgg caaccgcggc ggccgcgtcg cacgctgcaa accgtcaagc
     7081 cagccgagct cctggccgag cacctccttg tagaggaaca gcagcgccga cagggcttga
     7141 ttctgcgtcg aggccgccac gcggcgctcg cttgccaagt ggttcaggaa cgaggtgact
     7201 tcgggctcgc cgaccgagag cggatggcgt ttgcccgtaa agtagatgaa gcgcttgatc
     7261 caatgaacgt acgcttcctc ggtccggcgg ctgaagtagc ggcggcgaat cgcgtcgtgg
     7321 acctgatcga gcaggcgcgg gcgcccgggc atcgccgtcg tccctcctcc ggaacccttt
     7381 gattcttccg tcatccgttc ccccgagtcc gtctattctg gatatttctg actccactcc
     7441 ggctatcttg gct
//
LOCUS       DBNGWZ010000653         6312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_367641, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000653 DBNGWZ010000000
VERSION     DBNGWZ010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_367641"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="AC16CX_20425"
     CDS             <1..131
                     /locus_tag="AC16CX_20425"
                     /inference="COORDINATES: protein motif:HMM:NF023513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3567 family protein"
                     /protein_id="HIR3014906.1"
                     /translation="GDVAERFAHSLEAAVAEDASVERVDEFLGGFDVLLNQPLVIH"
     gene            complement(460..1422)
                     /locus_tag="AC16CX_20430"
     CDS             complement(460..1422)
                     /locus_tag="AC16CX_20430"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014907.1"
                     /translation="MKIRCLGMLACAVLLPSLAAAQAYPAKPVRMIIPFPPGGPTDLM
                     GRMAAERLTRAWGVQVVADNRAGAGGNIGTEQCAKSPPDGYTICMMTVAQSISPSIYS
                     KLGFDPLKDFSHVTLMAILPSMLTVHPTLPVKNVKELVALAKRQPGQLIYASTGNGTS
                     PHMLMEMFKFMAGVNMVHVPYKGQAPAVVDQMAGQVQLAFNTAVTVLPHVKAGKLKPI
                     AISTKERFPPMPDLQTVDEGGVKGFDGSSWQAVVMPAGTPKEIVARVYQELSVMLRAP
                     ETRERFLAQGALASGITPDEFLAFVKRELEKWARVAKAANVKLG"
     gene            complement(1513..2232)
                     /locus_tag="AC16CX_20435"
     CDS             complement(1513..2232)
                     /locus_tag="AC16CX_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3014908.1"
                     /translation="MMNLLGALSRHATPVNLKQLATETRLHPSTAHRILSVMVENRLV
                     DRIEPGTYRLGIRLLELGSLVKSRISVRQEALPHMQSLHQELGETVNLSVRHDDEVVY
                     IERTTGNSSMMRVVQIIGARAPLHITAVGKIFLADDGPDKCIEYARRTGLPKYTDNTL
                     IDADSLARELEKIRRQGYAFDNEEAEKGVSCIGAGIYNDEGRLVAGLSVSAPSDRLDK
                     SWAVQVRQTAEKISHAIGHQA"
     gene            2450..3037
                     /gene="greB"
                     /locus_tag="AC16CX_20440"
     CDS             2450..3037
                     /gene="greB"
                     /locus_tag="AC16CX_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HIR3014909.1"
                     /translation="MAKTAVLARNRNRNGNGRNGNGRNGTAAIRAEQEVKNYITPQGY
                     VRLKSELSKLLDVERPELVKTIAWAASNGDRSENGDYIYGKKRLREIDRRIHYLSKRL
                     DSAEVVDPARRGGSDQIFFGASVTYAYVRGGRDERTITIVGVDEVDVKRGQVSWLSPI
                     ARALLKKRSGDVVILATPSGEAEIEIIGVRYRQPA"
     gene            3292..4320
                     /locus_tag="AC16CX_20445"
     CDS             3292..4320
                     /locus_tag="AC16CX_20445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3014910.1"
                     /translation="MRATARSAAGGVIGRTPIVTVSFFTAALSAWRAAHLAVPDLRAD
                     DAPLEAVVRLSIKAVENARTAENLGAEREGTGVVFGDKGLILTIGYLILEAGSILVIA
                     GDGRVYPASVLGYDHATGFGLLRAGMDCRPLELGNSGSVRELATVLVAAHGTAGGASR
                     ACVVSRRRFTGWWEYMIEGGIFTAPPRFEHSGAGLLDADGKLVGIGSLWVSDALEIGA
                     AFPGNMFVPVDLLKPLLGDLLATGRRREPARPWLGVYSEEIQGHVVVTRVLPESPAEQ
                     AGLSRGDIILGVGGEAVGRQSEFYQRLWSSGEAGSPVVLHVLHKRTVRQLTVRSMDRM
                     AFLRPWPI"
     gene            4368..5396
                     /locus_tag="AC16CX_20450"
     CDS             4368..5396
                     /locus_tag="AC16CX_20450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3014911.1"
                     /translation="MTRRRLLFAMFAIGTLAGCAQAPIALKEMGSFHVGGREVVISGK
                     PVKEIVFTPGGVPAKVDPNGVYQVEQMYVQYFIPQNQRGALPLLMWHGGGLTGVTYET
                     TPDGREGWLNYFIKKGWAVYNSDAVERGRSGWAMYPDIFKGEPVFLTKENPFERFRIG
                     AGPGSYSKEPAKMKLLPGSQFPAEGYDNFTKQGVPRWTTTDPAIIAAYTELVDKVCPC
                     VVLIHSQSGQFGLRVAQARPDKVKALVLVEPAGVGDRKQVDRLKNVPILAVYGDYIDS
                     DARWPKIKANGVQFLEEVRKAGGRYEIVDLPKIGIRGNSHMMMMDRNSDQIAGVIQGW
                     LEKQGLYR"
     gene            complement(5501..>6312)
                     /locus_tag="AC16CX_20455"
     CDS             complement(5501..>6312)
                     /locus_tag="AC16CX_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3014912.1"
                     /translation="AAVGGAIAQTPLERGSYLVNAVMACDGCHTPRDKSGFVMEKRFS
                     GGSQTWDTPAYTVKGSNITPDRETGIGTWSENDIKRSLTEGVRPTGVPLAPQMPFAFY
                     KILTPRDLDAVVAYLRSLAPVRNEVQPPVYKAAMHTELVPSAVKSFAEGDLRDPIKRG
                     FYLATIAHCMECHGRRPDGVQDYKNGWGKGGYVFKGPWGSAVVPNITSHPKSGIGAWT
                     DEEIKRVLTHGVSRDGRAFKQPMARQDYFSRMTEADLDALVAWIRTIPPLE"
BASE COUNT         1081 a         1987 c         2084 g         1160 t
ORIGIN      
        1 agggtgatgt cgcggagcgg ttcgcgcatt cgctggaggc cgccgtcgcc gaagacgctt
       61 cagtcgagcg cgtggacgag ttcctcggcg gcttcgacgt acttctgaac cagccgctcg
      121 tcatccattg accggcgcgg ccgcgccggg gaaaagcgtg agtagtgagt agtgagtggt
      181 gagtggaacg gctgtgtacg gccatccgcc cgcagcagca gactctactc actactcacc
      241 aatcacgcat tatcagtcaa ggcgcaacta gccgggttcg tcgcggtcca gcatgcgcct
      301 gagcgcggcg aagtgcagat gccttcgccg aagcccaggc ggttcgccca gcgcagcgct
      361 gccgccaggt cgatacgcgc ctgcaattcc ggtttggggt ttcgggttcc gggtttcgaa
      421 ttacgcctca cccctctcgc ctctcccctc acggcctggt cagccgagct tgacgtttgc
      481 ggccttggcg accctggccc acttttccag ctcgcgcttg acgaaggcca ggaattcgtc
      541 cggcgtgatg cccgaggcga gcgcgccctg cgcgagaaac cgctcccggg tttccggggc
      601 cctgagcatg accgagagtt cctgatagac cctggcgacg atttccttcg gcgtgcccgc
      661 cggcatcacc accgcctgcc acgagctgcc gtcgaacccc ttcacgccgc cttcgtccac
      721 cgtctgcaga tccggcatgg gtggaaaacg ctccttggtg gagatggcaa tgggcttgag
      781 cttgcctgcc ttgacgtgcg gcagaacggt gaccgcggta ttgaaagcga gctgaacctg
      841 gcccgccatc tggtccacca ccgctggcgc ctggcccttg tagggaacgt ggaccatgtt
      901 gacgcccgcc atgaacttga acatttccat cagcatgtgc gggctggtgc cgttgccggt
      961 ggaagcgtag atgagctgtc ccggctggcg cttggcgagc gcgacgagct ccttcacgtt
     1021 cttcacgggc aacgtcgggt gcaccgtgag catgctgggc aggatcgcca tcagcgtgac
     1081 gtgcgagaag tccttcaacg ggtcgaaacc gagcttgctg tagatgctgg gcgagatgct
     1141 ctgcgccacc gtcatcatgc agatggtgta gccatcgggc ggcgacttcg cgcactgctc
     1201 ggtgccgata ttgccgccgg cgccggcccg gttgtcggcc accacctgca cgccccacgc
     1261 cctggtcagg cgctcggccg ccatacggcc catgaggtcg gtcggtcccc cgggcgggaa
     1321 cgggatgatc atgcgcaccg gcttggcggg ataggcctgc gcagccgcaa gtgacggcaa
     1381 cagaacggcg cacgcaagca tcccgaggca acggatcttc atggtgaaag ctcttcctgg
     1441 aatgagcgaa agcatgaatc gtggccggtc aacgagtagg aaaagccgat tcacgactca
     1501 cgattcacgc cattacgcct ggtgcccgat ggcgtgcgag attttctcgg cggtttgccg
     1561 cacctggacg gcccagctct tgtcaagccg gtcggagggg gcggaaacgg agaggccggc
     1621 gacgagcctg ccttcatcgt tataaatgcc ggcgccgatg caggagacgc ctttctcggc
     1681 ctcctcgttg tcgaacgcat agccctggcg gcggatcttc tccagttcgc gcgcgaggct
     1741 gtctgcatcg atcagggtgt tgtcggtgta tttcggcaag ccggtgcgcc gcgcatattc
     1801 gatgcacttg tcgggcccgt cgtcggccag gaatatcttg cccaccgcgg tgatgtgcag
     1861 gggggcgcgt gcgccaatga tctgcacgac tcgcatcatc gagctgttgc cggtggtgcg
     1921 ctcgatgtaa accacctcat cgtcgtggcg caccgacagg ttgacggtct cgcccagctc
     1981 ctggtgcagg ctctgcatat gggggagggc ctcctggcgc acgctgatac gcgatttgac
     2041 caggctaccc agctccagca ggcggatgcc gagccgatag gtgccgggct cgatccggtc
     2101 caccaagcgg ttctccacca tgaccgagag gatgcggtgc gcggtggacg ggtggagccg
     2161 ggtttcggtg gcgagctgct tcaagttgac cggtgtggcg tgccgggaaa gcgcgccgag
     2221 caggttcatc atgcgttcga tgacctggat cgaggacttg gctgcttttt gtgccacagc
     2281 agagggttgt tccgactttg gcgttcgatt ttatattctg aaatccgcag ctacaaccaa
     2341 aaacgccgat ttcggggctg catttcgagc cctaccacag gtatagccac taatacattg
     2401 tttttgctat aatatgaggc tcctccgcct gattcgcatc acacttacca tggctaagac
     2461 agcggtactc gcacgtaatc gtaatcgcaa tggcaacggc cgcaacggca acggccgcaa
     2521 cgggacggcg gcaatacggg cggagcagga agtcaaaaac tacatcacac cgcagggtta
     2581 cgtgcggctc aagtccgaac tttccaagct gctcgacgtc gagcgtcccg agctggtgaa
     2641 gaccatcgcg tgggctgctt ccaacggcga ccgctccgag aacggcgact atatctacgg
     2701 caagaagcgg ttgcgcgaga tcgaccgccg cattcactat ctgagcaagc gcctcgacag
     2761 cgccgaagtg gtggacccgg cgcggcgcgg cggatccgac cagatcttct tcggtgcgtc
     2821 cgtcacttat gcctacgtgc ggggcgggcg cgatgagcgc accatcacca tcgtgggcgt
     2881 ggacgaggtg gacgtgaagc gcggacaagt gagctggctc tcgcccattg cccgggcgct
     2941 gctcaaaaag cgctccggtg acgtggtgat actggccacc ccctctggcg aagccgaaat
     3001 cgagatcatc ggcgtccgtt accggcagcc cgcctgaggg cggccggtct ccctttacgg
     3061 tgttataggc ggccggcctg cggcacccgt tgccgtgcgg catgcgcacc gctaacattg
     3121 gttaaacccg gacgcactgc gccggtttaa cttcgcgtgt tcgaacggac aggattgctg
     3181 ccgttcaaca cgcggctgaa tccggacgcc aagcgccggc ttaactccgc gcgttcgaat
     3241 ggacgggatg gccgccgttc aacacgcggg ttgttgccgc cagctgacgc cgtgcgcgca
     3301 accgcgcgca gcgcagccgg tggtgttatc gggcgcacac ccatcgtgac cgtttccttt
     3361 ttcaccgccg ccctgtccgc ctggcgcgcc gcccatctcg ccgttcccga cctgcgcgcc
     3421 gacgatgccc cgctcgaggc ggtggtgagg ttgagcatca aggcggtgga aaacgcgcgc
     3481 accgcagaga acctgggcgc ggagcgcgaa ggcaccgggg tggtgttcgg cgacaaaggg
     3541 ctgatcctca ccatcggtta cctcattctc gaggcgggtt cgattctggt catcgccggc
     3601 gacgggcgcg tttacccggc gagcgtgctc ggctacgacc acgccacggg attcggcctg
     3661 ttgcgcgccg gtatggactg ccggcccctt gagctgggca actcgggaag cgtgcgcgag
     3721 cttgccaccg tactggtcgc cgcccacggc accgccgggg gtgcctcgcg cgcctgcgtg
     3781 gtatcgcggc ggcgcttcac cgggtggtgg gagtacatga tcgagggcgg gatcttcacc
     3841 gcgccaccgc gcttcgagca cagcggggcg gggctgctcg atgccgacgg caagctggtg
     3901 ggcatcggct cgctgtgggt gagcgacgcg ctcgagatcg gggccgcctt tcccggcaac
     3961 atgttcgttc ccgtagactt gctgaagccg ctgctcggcg acctgctcgc gaccggccgc
     4021 cgccgcgagc ccgcacggcc ctggctcggc gtctattccg aggagatcca gggacacgtc
     4081 gtggtgacgc gggtgttgcc cgagtcgccg gcggagcagg cggggctttc ccggggtgac
     4141 atcatcctcg gggtgggagg cgaggcggtc gggcgccaga gcgagtttta tcaacggcta
     4201 tggtcgagcg gggaggccgg ttccccggtc gtgctgcacg tgctgcacaa gcggacggtg
     4261 cggcagctca cggtccgctc gatggaccgg atggcgttcc tgaggccctg gcccatctaa
     4321 gccgtcccaa tacccgtcac acttttcttt cgaatcggag gcgttagatg actcgacgca
     4381 gactgttatt cgcgatgttc gctatcggaa cgctggcggg ttgcgcgcag gcgccgatcg
     4441 cgctgaagga gatgggctcg ttccacgtcg gcgggcgcga ggtcgtgatc tccggcaagc
     4501 cggtgaagga gatcgtgttc acaccgggcg gcgtgccggc caaggtggac cccaatggcg
     4561 tttaccaggt cgagcagatg tacgtgcagt acttcatccc gcagaaccag cgcggcgcgc
     4621 tgccactgct gatgtggcac ggcggcggac ttaccggcgt cacctatgag acgacgcccg
     4681 acggccgcga gggctggctc aactacttca tcaagaaggg ctgggcggtc tacaactccg
     4741 acgcggtcga gcgcgggcgt tccggctggg ccatgtatcc cgacatcttc aagggcgaac
     4801 cggtgttcct gaccaaggag aacccgttcg agcgcttccg catcggcgct ggccccggct
     4861 cctatagcaa ggagccggcg aagatgaagc tgttgcccgg cagccagttt ccggccgagg
     4921 gctacgacaa cttcaccaaa cagggcgtgc cgcgctggac cacgaccgat ccggcgatca
     4981 tcgccgcgta caccgaactg gtggacaagg tgtgcccgtg cgtcgtgctg atccactcgc
     5041 aatcggggca gtttggactc agggtcgcgc aggcgcgtcc cgacaaggtg aaggcgctgg
     5101 tgctggtcga acccgccggc gtaggcgacc gcaagcaggt ggaccgcctg aagaacgtgc
     5161 cgatcctcgc ggtttatggc gactacatcg actccgacgc gcgctggccc aagatcaagg
     5221 caaacggcgt gcaattcctc gaggaagtgc gcaaggccgg cggccgctac gagatcgtgg
     5281 acctgcccaa gatcggcatc cgcggcaact cgcacatgat gatgatggac cggaacagcg
     5341 accagatcgc cggcgtaatc cagggctggc tggagaagca aggcctgtac cggtaagccg
     5401 cgccgccgca gtcagcagag tctgttttcg aaccgagttc gacgacaaac tcgttacctc
     5461 gctcgggatt cgccagtaag tcccgacttc acgccaccgc tcattcaagc ggcgggatcg
     5521 tgcgaatcca ggcgacaagg gcgtcaaggt cggcttccgt catgcggctg aaatagtcct
     5581 ggcgggccat cggctgcttg aacgcccggc cgtcgcgcga tacgccgtgc gtgaggacgc
     5641 gcttgatctc ctcgtcggtc catgcgccga tgcccgactt cgggtgcgac gtaatattgg
     5701 ggactaccgc agagccccac ggacctttga atacgtagcc gcccttgccc cagccattct
     5761 tgtagtcctg gacgccgtcc ggccgccgcc catggcattc catgcaatgg gcgatcgtgg
     5821 cgagatagaa gccgcgcttg atcgggtcgc gtagatctcc ctcggcaaac gatttcaccg
     5881 cgctcggaac gagctcggtg tgcattgccg ccttgtagac cggcggctgg acctcgttgc
     5941 ggaccggcgc gagggaacgc aggtaggcca ccaccgcgtc cagatcgcgc ggcgtcagta
     6001 tcttgtagaa ggcgaagggc atttgcggcg cgagcggcac gccggtcggc cggacgccct
     6061 ccgtgaggga gcgcttgatg tcgttttcgc tccacgtgcc gatcccggtc tcgcgatcgg
     6121 gtgtgatgtt ggagcccttg acggtgtagg cgggcgtgtc ccaggtctgt gatccacccg
     6181 agaaacgctt ttccatcacg aaaccgctct tgtctctcgg cgtgtggcag ccgtcgcagg
     6241 ccatgacggc gttgacgaga tagctgccac gctcaagcgg tgtctgggcg attgcaccgc
     6301 caaccgctgc ga
//
LOCUS       DBNGWZ010000654         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2001924, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000654 DBNGWZ010000000
VERSION     DBNGWZ010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2001924"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..151
                     /locus_tag="AC16CX_20460"
     CDS             35..151
                     /locus_tag="AC16CX_20460"
                     /inference="COORDINATES: protein motif:HMM:NF021772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HIR3014913.1"
                     /translation="MLWNAHPRVFLPIEKTGEALCPYCGTRYTLKGGAPAGH"
     gene            273..1286
                     /gene="waaF"
                     /locus_tag="AC16CX_20465"
     CDS             273..1286
                     /gene="waaF"
                     /locus_tag="AC16CX_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR3014914.1"
                     /translation="MRRILVVGPSWIGDTVLAQPLFKLLHARHAGLALDVLAPQWTLP
                     LLERMPEVRRVIASPFVHGEFRLGERRRLGRDLAQEDYDQAIVLPNTFKSALAPFFAG
                     IRARTGYVGELRYGLLNDARRLDKKRLPQIAQRYAALALPRGEVPRLPLPALALRVEE
                     TARRAALDRLGLDRSRPAAVLCPGAEFGPAKRWPARYFAELARGFAARGCAVWLIGSA
                     NDRTVGDEIAQAAGGICRNLCGDTTLTEAIDVLASSALVVSNDSGLMHVAAALGRPLI
                     ALYGSSSPAFTPPLSPTAQILKLDLPCSPCFQRVCPLGHFNCMVQLTPDRVLAAVDFD
                     RIP"
     gene            1324..1764
                     /locus_tag="AC16CX_20470"
     CDS             1324..1764
                     /locus_tag="AC16CX_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR3014915.1"
                     /translation="MKKALFATPQDAEAAFYEALTKSDLEAMMAVWADDDDVYCVHPN
                     GARISGVEQVRESWRKIFASGQTLRFQLREQQYVQGMMLSVHSVYEHITVAGEPRARG
                     PVIATNVYLRTERGWRMVAHHASPAPATAVAEPEPRRATPKTLH"
     gene            1834..>2113
                     /locus_tag="AC16CX_20475"
     CDS             1834..>2113
                     /locus_tag="AC16CX_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014916.1"
                     /translation="MSNTDFGKPDVIVVGAGNAACCAALAAREGGASVIILEAAPIED
                     CGGNSRYTAGLMRVVYNGVDDLAQLIPDLTDEEKKTHDFGSYTAEHYYD"
BASE COUNT          338 a          741 c          703 g          331 t
ORIGIN      
        1 ggacctgccg ctgcactgcc ccatgccgtc catgatgttg tggaacgcgc acccgcgcgt
       61 gttcctgccg atcgagaaga ccggggaggc gctgtgcccc tactgcggca ctcgctatac
      121 gctcaaaggc ggtgccccgg cgggacacta gtcccgccaa aggcggaagg atgaaggcgg
      181 aaggcggaag gaaagcgaca accttgcgcc ggcggcgccc aacctcttcc gactttcgcc
      241 tttcgccttt cgccttgtag ggccgacaac gtatgcgacg catactcgtt gtcggcccct
      301 cctggatcgg cgacacggtg ctggcgcagc cgctgttcaa gctgctgcat gcgcgccacg
      361 cgggtctcgc gctcgacgtg ctcgccccgc aatggacgct gcccctgctc gaacgcatgc
      421 ccgaagtgcg gcgggtgatc gcaagcccct tcgtccacgg cgaattcagg ctcggggagc
      481 gccgccgcct gggacgcgac ctcgcgcaag aggactacga ccaggcgatc gtgctgccca
      541 acaccttcaa gtccgcgctg gctccgtttt ttgccggcat ccgggcgcgc accggttacg
      601 tcggcgagct gcgctacggc ctgttgaacg acgcgcgccg cctggacaag aagcgcctgc
      661 cgcagatcgc gcagcgctac gcggcgctcg ccctgccccg cggcgaggtg cccaggttgc
      721 cgctgcccgc cctcgccctg cgggtggagg agacggcacg gcgcgcggcg ctcgaccggc
      781 tcggccttga ccggagccgg ccggcggcgg tgttgtgccc gggggcggag ttcggcccgg
      841 ccaagcgctg gccggcgcgc tatttcgcgg agctggcccg gggattcgcc gcgcgcggct
      901 gcgcggtctg gctgatcggc tcggccaacg acaggacagt gggagacgag atcgcgcagg
      961 ccgccggcgg catctgccgc aatctgtgcg gcgacaccac gctcaccgag gcgatcgacg
     1021 tgcttgcctc gagcgcgctg gtggtgagca atgattcggg tctgatgcac gtcgcggccg
     1081 cgctgggcag gccgctcatc gcgctctacg gctccagcag cccggcgttc accccgccgc
     1141 tttcgccaac cgcgcagata ctcaagctcg atcttccctg cagcccgtgc tttcagcgcg
     1201 tatgccccct cgggcacttc aactgcatgg tgcagctcac cccggaccgg gtcttggccg
     1261 ccgtcgattt tgatagaatt ccgtgacccc tcgcctcgca gccaacaccg cggtcccgcc
     1321 ggcatgaaga aagccctgtt cgccactccg caggacgccg aggccgcgtt ctacgaggcc
     1381 ctcaccaagt ccgacctgga ggcgatgatg gcggtgtggg ccgacgacga cgacgtctat
     1441 tgcgtgcatc ccaatggagc gcgcatttcc ggcgtcgagc aggtgcgcga atcgtggcgg
     1501 aagatcttcg ccagcgggca gacgctgcgt ttccagttgc gcgagcagca gtacgtccag
     1561 ggcatgatgc tgtcggtgca cagcgtctac gagcacatta cggtcgcggg cgaaccgcgc
     1621 gcgcgcggcc cggtgatcgc gaccaacgtg tacctgcgga ccgagcgcgg ctggcgcatg
     1681 gtcgcgcacc acgcttcgcc ggcgcccgcc acggcggtgg ccgagcccga gccgcggcgc
     1741 gcgacgccaa agacgctgca ctagaacccg tggttcgtga atcgtgagtc gtgactcgat
     1801 tcaaaaccca ttctgagacc tgaaaggaat gcaatgagca acaccgattt cggcaagccc
     1861 gatgtgatcg tcgtcggcgc cggcaacgcc gcctgctgcg cggcgctcgc cgcgcgcgag
     1921 ggcggcgcaa gcgtcatcat cctcgaagcc gcgccgatcg aggactgcgg gggcaacagc
     1981 cgctatacgg cgggcctcat gcgcgtggtc tataacggcg tggacgacct cgcgcagctc
     2041 attcccgacc tcaccgacga ggagaagaag acccacgact tcgggtccta caccgccgag
     2101 cattactacg acg
//
LOCUS       DBNGWZ010000655         5059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_489816, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000655 DBNGWZ010000000
VERSION     DBNGWZ010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_489816"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2064)
                     /locus_tag="AC16CX_20480"
     CDS             complement(<1..2064)
                     /locus_tag="AC16CX_20480"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3014917.1"
                     /translation="MNRAAPISVGQILTGSLFNEPMRVVTVQDQGTNSWVAGLVGVRT
                     ERFRSVALSPADLKTLTITSTDYTFDGDGQLLRLGLQAYALGIAWEFDPYFGLSISRV
                     DPLPHQLAAVYDHLLKLARVRFLLADDAGAGKTIMSGLLIRELKLRGLAERILIVCPA
                     NLAFQWQRELNEKFDEEFVVLKGNDIRDQFGVNQWMKQRQVITSLDLAKREDVLPGLH
                     QVHWELVIVDEAHRMSWTPPARKTARYALGELLRESADHFLLLTATPHKGNPENFTLF
                     LQLLDRDVYADVRSIEQAMDRRAAPFYLRRTKEAMVYFPERQSDGSWVARKIFTKRIP
                     RTVEFQIDGAEFELYRDVTRFVKRQSARAALEGDDPRARAVGFLMALYQRRLASSTRA
                     MRQSLENRARRLDDGLKRAKEIALTAPPDLPDEEELEEMEEGEREHLERILEAITLAG
                     NANQVREEISELLALAKQAKDIEDKSVEAKLSRLKDLLKTEGFFDKPEQRLLIFTEFK
                     DTLDYLVEKLKAWGFRVGFIHGGMKAGSREEPASRLYAEQQFKDGAIQVLVATEAAGE
                     GINLQCCHILFNYDIPWNPNRLEQRMGRIHRYGQVHDCLIFNFVATNTIEGHVLRRLL
                     EKLQEIRDALDDDAVFNVVGEVLPAAHVERVLRDYYAGKLGDSDLEDRLLREVDEGQF
                     RAICQN"
     gene            2591..3556
                     /locus_tag="AC16CX_20485"
     CDS             2591..3556
                     /locus_tag="AC16CX_20485"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR3014918.1"
                     /translation="MRTYKQLTLEQRYQIDALLRAGRSCPHIARTLGVHRCTVYREVK
                     RNRSRKGYVSHRAHGRAVRRRKSKARRRLQEHHWATVRRLLGQDWSPEQISRRLALEG
                     GFRISHEWIYQFLLIDKRTGGTLYRYLRCQKQHRKRYGKHHRMRGGIVGRVGIAQRPS
                     VVEQRRRLGDWEADTLAGRRWSTRILSLVERKSRYTVLGHLANKSAPHTTQCLLSCLR
                     AQPHPVQTITVDNGQEFAAHRHITQALQARVYFSDFHAPWQRGTVENINGLLRQYFPK
                     GHDFKRLRRQDLLNAQTRLNSRPRKCLGFRTPYEIFFATTVALTS"
     gene            complement(3620..>5059)
                     /locus_tag="AC16CX_20490"
     CDS             complement(3620..>5059)
                     /locus_tag="AC16CX_20490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3014919.1"
                     /translation="SANMALCLCPMLTSGVLEALKIHASPEQRKAWMPKLTSGEWTGT
                     MNLTEPQAGSDLSAVRTRALREGGYYRIQGTKIFITWGEHDMAENIVHLVLARTPDAP
                     EGVKGISLFVVPKFLPKPDGTPGERNDVRCVSIEHKLGIHASPTCVLAYGDQKGAIGY
                     LVGEENRGLEYMFTMMNHARLAVGVEGVAISERAYQHALDYARTRVQGREIGQRGGDR
                     VTIIYHPDVRRMLMSMRAQTEAMRALAYFAAATLDKARRHPDPAEKRRSQALLDLMTP
                     IVKGWCTEQGVEVASTGIQVHGGMGFVEETGAAQYLRDARITTIYEGTTGIQANDLVG
                     RKVGFEKGATALAVIEAMRGIDARLAARGEMLAAHRVNMKEALDGLDGATRWILETFP
                     KSPKAVAAVSVPYLKLFGTVAGGWMMARAALTAQDRLEQPDADRDFLQGKLATARFYG
                     EHELPKALSLAREVTRGADSVLALDPAKF"
BASE COUNT          917 a         1615 c         1545 g          982 t
ORIGIN      
        1 gttctggcag atggcgcgaa actgtccctc gtccacttcg cgcaggagac ggtcctcaag
       61 atcagaatcg ccgagcttcc cagcatagta gtcgcgcagt acccgctcga catgcgcggc
      121 cggcagcacc tcgcccacga cattaaatac cgcgtcgtcg tcgagcgcat cgcgaatctc
      181 ctgcaacttc tccagcaacc gccgcagcac gtgaccttcg atggtattgg tcgcgacaaa
      241 gttgaagatc agacaatcgt gtacctgtcc gtagcggtgg atgcggccca tgcgctgctc
      301 gagccggttc gggttccagg gaatgtcgta attgaacagg atgtggcaac actgaagatt
      361 gatgccctcg cccgctgctt cggtggccac gagtacctgg attgccccgt ctttgaattg
      421 ctgctcggcg tagaggcgtg aggccggctc ttcgcgcgac ccggccttca tgccgccatg
      481 gatgaagccg acgcggaatc cccatgcttt gagcttctcg accaagtagt ccagcgtgtc
      541 cttgaactcg gtgaagatga gcaaccgctg ctcaggctta tcaaagaaac cctccgtttt
      601 cagaaggtcc ttcaggcggg aaagtttggc ctcgacactc ttgtcctcga tatccttggc
      661 ctgtttggcg agcgcgagca gttctgaaat ctcctcgcgt acctggttcg cattacccgc
      721 cagcgtaatc gcctccagaa tgcgctcgag gtgctcgcgc tcaccctctt ccatttcctc
      781 gagttcttcc tcatccggga ggtcaggagg cgcggtcagt gcaatttcct tggcgcgctt
      841 caggccatcg tctagccggc gtgcacggtt ctcaagtgac tggcgcatgg cgcgcgtgct
      901 ggaagcgagc cttctttggt acagcgccat gaggaagccg accgcacgtg cccggggatc
      961 gtcaccttcc aacgcagcgc gagcgctctg gcgcttcacg aaccgtgtta cgtctcggta
     1021 aagctcgaac tccgcgccat ctatctgaaa ctccacggtg cggggtatgc gcttggtgaa
     1081 tatcttccgc gcaacccacg aaccatccga ctggcgctca gggaaataga ccatcgcctc
     1141 cttggtgcgg cgcaggtaga acggcgccgc tcgccgatcc atcgcctgtt cgatcgaccg
     1201 cacgtctgcg tagacatcac gatcaagtag ttgcaggaat agcgtgaaat tctccggatt
     1261 acccttatgc ggcgtcgcgg tcaggagtag aaagtggtcg gcggactcgc gcaacagttc
     1321 gccaagcgca tagcgcgcgg tcttgcgcgc cggaggcgtc cacgacatac ggtgcgcttc
     1381 gtcgacgatc accagttccc aatgcacctg atgtaggccc ggtagcacat cttcacgctt
     1441 ggccagatcg agcgaggtga tgacctgcct ctgtttcatc cactgattga cgccgaactg
     1501 gtcgcggata tcgttgccct tgaggacaac gaattcttca tcgaattttt cattcagctc
     1561 gcgttgccac tggaatgcga gattggcggg gcagacgatc aaaatccgct cggcaagacc
     1621 ccggagcttc aattcacgga tgagcaaccc ggacatgatc gtcttgcccg ccccggcgtc
     1681 atcggcgagc aggaatcgta cgcgcgcaag cttgagcaaa tggtcgtaca ccgctgcgag
     1741 ctggtggggc agtgggtcga ctcgcgaaat ggacagcccg aagtaggggt caaattccca
     1801 cgcaatgccg agtgcgtagg cctgaaggcc gaggcgaagt agttgtccgt ccccgtcgaa
     1861 tgtgtagtcg gtgctcgtga tcgttagcgt cttgagatca gcagggctca gcgccacgct
     1921 cctgaagcgc tccgtccgga cgccgaccag tcccgcgacc caagagttgg taccctggtc
     1981 ttgcacggtc acgacgcgca tcggctcgtt gaaaagcgaa ccggtgagga tctgaccaac
     2041 gctgatcgga gcggctcgat tcatgcgctg gttcgcccaa cgagaccgct accggagtag
     2101 cgggcgcctt cggccatgcg ccagagttcg gcctcatagc caacgtttgc accgttactc
     2161 ttggattttt cggagctttt gcgagccatc tcccctcccc gccgaagaat gatacggtgc
     2221 ccagtgactg gtgaccagtc agggcaacga gctacggatt ttgatcttgg ggcccctgag
     2281 agtcgccgag cagcgcagac cggcccgggg tttcggtcga gcactgggcg agcctcccgt
     2341 acccatgcgt tcccgcattg atttcccgcc tgatgcgctt gaaaaaacat gagcggtgcg
     2401 cgagttgcgc agaccgccgg gctggacgag cagcacaggg gaccgcacga gcttgcgagg
     2461 tagcggcggc gaaccgggga tcccctttct ttggtgactg ccggattcaa ctctcaagtg
     2521 caactttgaa gcgaggcggg tgagctgtgt agcatcgcag ccgcttgatc gccaaatcaa
     2581 aggagccttg atgagaacct acaagcagct caccctggaa caacgatatc agattgatgc
     2641 gttattgcga gccggacgtt catgccctca tatagctcgc acgctgggcg ttcatcgttg
     2701 cactgtctat cgggaggtta agcgcaaccg ttcccgcaag ggatatgtat cgcaccgagc
     2761 gcacgggcgg gccgtgcggc gcaggaagag caaggccagg cgcaggctgc aggaacatca
     2821 ttgggcgacg gtacgacgcc tcttgggcca agactggagt cccgagcaga tttcccgccg
     2881 gctggccctg gagggtggtt ttcgcatcag ccacgaatgg atttatcagt tcctgttgat
     2941 cgacaagcgc accgggggca ccctctaccg ctatctgcgc tgccagaagc agcaccgcaa
     3001 acgctacggc aagcaccatc gcatgcgtgg cggtatcgtc gggcgggtcg ggatcgcgca
     3061 gcgcccaagc gtcgttgagc aacgccgtcg attgggcgac tgggaggccg atacgctggc
     3121 cggccgtcgc tggtccacgc gcatcctgag cctggtggag cgcaagtccc gctatacggt
     3181 gctgggacac ctggctaaca agtcagcgcc ccataccacc caatgcctgc tctcttgcct
     3241 aagagctcaa ccccatcccg tgcagaccat caccgtcgac aacggtcagg agttcgcggc
     3301 ccaccggcac atcacgcaag cgctgcaagc ccgggtgtac ttctctgatt tccacgcccc
     3361 ttggcaacga ggcaccgttg agaacatcaa cgggctgctg cgacagtatt tccccaaggg
     3421 acatgatttc aagcggcttc gtcgccagga tctactgaac gctcagactc ggctcaatag
     3481 ccggccgcgt aagtgtttgg gattccgaac cccgtatgag atattctttg ccaccaccgt
     3541 tgcactaacg agttgaatcc ggcactttct tttatgggcg agcaaaaaga aagtcaccgg
     3601 caccggggcc gccccccggc tagaacttcg cgggatccag cgccaagacg ctgtcggccc
     3661 cccgcgtcac ctcgcgcgcg agtgacaggg ccttgggcag ctcatgctcg ccgtagaagc
     3721 gcgccgtggc gagcttgcct tgcaggaaat cgcggtcggc atccggctgc tcgagccggt
     3781 cctgcgcggt gagtgccgcg cgcgccatca tccagcctcc cgccaccgtg ccgaagagct
     3841 tcaggtacgg caccgacacc gccgccaccg ccttggggct cttcgggaat gtctccagga
     3901 tccagcgcgt ggcgccgtcg agaccatcaa gtgcctcctt catattgacg cggtgggcgg
     3961 ccagcatctc acccctcgcg gcgagccgtg cgtcgatgcc gcgcatcgcc tcgatcacgg
     4021 ccagcgcggt cgcgcctttc tcgaagccca ccttgcggcc gacgagatcg ttcgcctgga
     4081 tgccggtggt gccctcgtag atggtggtga tgcgcgcgtc gcgcaggtac tgcgccgcgc
     4141 cggtttcctc gacgaaaccc atgccgccgt ggacctgtat gccggtggag gcgacctcca
     4201 cgccctgctc cgtgcaccag cccttgacga tcggtgtcat caggtcgagc agcgcctggc
     4261 tgcgccgctt ttccgcggga tcgggatggc gccgcgcctt gtcgagcgtc gcggccgcga
     4321 aataggcaag cgcacgcatc gcctcggtct gcgcccgcat gctcatcagc atccggcgca
     4381 cgtcgggatg gtagatgatg gtgacgcggt cgccgccccg ctggccgatc tcgcggccct
     4441 gtacgcgcgt cctcgcatag tcgagcgcgt gctggtacgc ccgctcgctg atcgccacgc
     4501 cctcaacgcc gaccgcgagc cgggcgtggt tcatcatggt gaacatgtac tcgagtccgc
     4561 ggttctcctc gcccacaagg tagccgatcg cgcccttctg gtcgccgtac gccagcacgc
     4621 aggtcgggct cgcgtggatg ccgagcttgt gctcgatcga aacgcagcgg acgtcattgc
     4681 gctcgcccgg cgtgccgtcc ggtttcggca ggaacttcgg cacgacgaag agcgagatgc
     4741 ccttcacgcc ttccggcgcg tcgggcgtgc gcgcgagcac gaggtgcacg atgttctccg
     4801 ccatgtcgtg ctcgccccag gtgatgaaga tcttggtgcc ctggatgcgg tagtagccgc
     4861 cttctcgcag cgcgcgcgtg cgcacggcgg agaggtccga gcccgcctgc ggctcggtga
     4921 ggttcatggt gccggtccat tcgcccgagg tgagcttggg catccaggcc ttccgctgtt
     4981 ccggggaagc gtgaatcttg agcgcctcga gcacgcccga ggtcagcatg gggcacaggc
     5041 acagcgccat gttcgcgct
//
LOCUS       DBNGWZ010000656         4093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_489891, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000656 DBNGWZ010000000
VERSION     DBNGWZ010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_489891"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="AC16CX_20495"
     CDS             <1..1115
                     /locus_tag="AC16CX_20495"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR3014920.1"
                     /translation="SAQGREAQPDTHHAGFSTRTVRLAWILAVAYLLVIVYASLLPFH
                     GWRMPPEEILRFLIASWPRHITPQDVAVNVAAYVPLGFLLSVGFGARYGPGRSVVAAV
                     FAGALLSLAMETTQMFLSSRTASNVDLLTNGLGALLGAMAAPLLAPTHVLGGKLHSLR
                     RHLFLEGMVADVGLVIIGLWLATQFHPTAQLFGTGSLRATFDLSAHFTHTPWLAFSSE
                     AVVAMFNVLGAGLMLSALMRDGARPMLVIGVMIGTAFAIKIFTAVALVQVPAPLAWLT
                     PGVLVGLAAAWALLYGTAHLPRRAQLAVSAACIVMATAAINFAPDNPYHNVPPRLLAR
                     GASHFLSFSGIVRALSELWPLLAIGFLLCALAARKE"
     gene            1189..1497
                     /locus_tag="AC16CX_20500"
     CDS             1189..1497
                     /locus_tag="AC16CX_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR3014921.1"
                     /translation="MTYYSRHVFFCCNQRENGEQCCNNNGSRKLREYAKDRVKSLKLS
                     GPGKIRINQSGCLDRCKEGPIMVVYPEAVWYTYVDQADIDEIIDEHLVNGRIVQRLRI
                     "
     gene            1627..2262
                     /locus_tag="AC16CX_20505"
     CDS             1627..2262
                     /locus_tag="AC16CX_20505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3014922.1"
                     /translation="MPPIEVRPLPAASLTRVMIEGPAGKIETDINDPGGAQRGIALIA
                     HPNPVQGGTKDNKVVTTLAKTFFALGYVTARPNFRGVGASEGKHDEGRGETEDLLAVA
                     GYLKRQYGGLPLVLAGFSFGSFVQTRVAKRLKPERMVLVGAAVNRFHAETVPHDTLVI
                     HGERDDVVPLQTVLDWARPQGLPVVVVPGGEHFFHGRLNLLADAVVRFFRG"
     gene            2295..3221
                     /locus_tag="AC16CX_20510"
     CDS             2295..3221
                     /locus_tag="AC16CX_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3014923.1"
                     /translation="MSLENAFYLRVSGLRKSYAGQEVVAGIDLELKPGECFGLLGPNG
                     AGKTTTLRLCLGLIDPDGGNIEVLGLPVPARAREARRRIGVVPQADNLDPDFTVEENL
                     LVYGRYFGLDEAEARGRIPALLDFAGLSARASSRIQTLSGGMKRRLTLARALINDPDL
                     IFLDEPTTGLDPQARHLIWERLRHLLARGKTIFLTTHFMDEAERLAHRLAIMDRGRII
                     ALGAPRELIVRHIEPQVVEVYGDGAERWAREFGRARSERCEQAGETVFCYARDAGPLV
                     QNLESQEELRYLHRHANLEDVFLKLTGRELRD"
     gene            3357..>4093
                     /locus_tag="AC16CX_20515"
     CDS             3357..>4093
                     /locus_tag="AC16CX_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3014924.1"
                     /translation="MITKTLALPAFSLRHLHVWRRNSLVWRKLAIPSMLGNLADPLIY
                     MLGLGYGLGAMLSDVGGMSYIAFLATGIVCSSTMMSASFEAMYSGFSRMHVQKTWDAI
                     MNAPVTLDDVVLGEAVWAASKSLLSGMAVLLVAWAIGLVHSGSALWAIPVVFLTGLAF
                     ASLGLVMTALSPSYDFFMYYFTLAITPMMLVSGVFFPVSQLPGPLQAVTAFLPLTHAV
                     ELVRPLMSGVTPAAIPLHLSVLAAYAVA"
BASE COUNT          640 a         1395 c         1299 g          759 t
ORIGIN      
        1 ggtcagccca ggggcgggaa gcgcaacccg acacgcatca cgccggattt tccacgcgca
       61 ccgtgcggct ggcgtggata ctcgccgtag cttacctgct cgtcatcgtg tatgccagcc
      121 tgctgccgtt ccatggctgg cgcatgccgc ccgaggaaat cctgcgcttc ctcatcgcgt
      181 cgtggccgcg ccacatcacg ccgcaggatg tggcggtgaa cgtggccgcc tacgtcccgc
      241 tcggcttcct gctctcggtc ggcttcggcg cgcgttacgg cccggggcgc agcgtggttg
      301 cggccgtctt cgccggggcg ctgttgagcc tggcgatgga aactacgcaa atgttcctgt
      361 cctcgcgtac cgcctccaac gttgacctgc tcaccaacgg cctcggcgca ttgctcggcg
      421 cgatggccgc gccgttgctc gcgccaacgc acgtcctcgg cggcaagctc cattccctgc
      481 gtcgccacct gttcctcgag ggcatggtgg ccgacgtcgg gctggtgatc atcggcctgt
      541 ggctcgccac gcaattccat cccaccgcgc agctgttcgg caccggaagc ctgcgcgcca
      601 ctttcgatct gtccgcgcac ttcactcaca cgccctggct cgcgttctcc agcgaggcgg
      661 tcgttgcgat gttcaacgtg ttgggcgcag gactcatgct gtccgcgctc atgcgcgacg
      721 gcgcgcgacc catgctcgtg atcggcgtga tgatcggcac ggcgttcgcc atcaagattt
      781 tcaccgccgt cgcgctggtc caggtgcctg cgccgctggc ctggctcaca ccgggcgtcc
      841 tggtcgggct cgccgccgct tgggcgctgc tctatgggac ggcgcacctg ccgcggcggg
      901 cgcagctcgc cgtttcggcg gcgtgcatcg tgatggcgac ggcggcgatc aacttcgctc
      961 ccgacaatcc ctaccacaac gtcccgccgc ggctgctggc gaggggagcc agccactttc
     1021 tgagcttctc cggcatcgtg cgggcgctct ccgaattgtg gccgttgctc gcgatcggct
     1081 tcctgctctg cgcgctggct gcccgtaagg aatgagcagg ccgggagcca agcgccgcgc
     1141 cggctgcggg tctgccgtat aattgcgcct cctcacatcc tggtacccat gacctactac
     1201 tccaggcacg tctttttctg ttgcaaccag agagaaaacg gcgagcagtg ctgcaacaac
     1261 aacggctcgc ggaagctgcg cgagtacgcc aaggaccgcg tgaagagcct caagctttcc
     1321 ggccccggca aaatccgcat caaccagtcg ggctgtctcg accgctgcaa ggagggtccc
     1381 ataatggtgg tctatcccga ggccgtgtgg tacacctacg tcgaccaggc cgacatcgac
     1441 gagatcatcg acgagcatct ggtcaacggc cgcatcgtcc agcggctgcg catctaggcc
     1501 ggagcgatat atctcgttgc tcgtcgctcg ttgctcgtga ctgctgaacg gtgttgactc
     1561 cgacgactcg cgagaccggg tttgacgagt aacgagaaac gagagacgag tcactctttc
     1621 aggctaatgc cgccgatcga ggtccgcccg cttcccgccg cctcgctcac gcgggtgatg
     1681 atcgaggggc cggcggggaa aatcgagacc gacatcaacg acccgggcgg cgcgcagcgc
     1741 ggcatcgcgc tgatcgcgca ccccaacccg gtgcagggcg gcaccaagga caacaaggtc
     1801 gtcaccacgc tcgccaagac cttcttcgcc ctgggttacg tgacggcgcg gcccaacttc
     1861 cgcggcgtag gggcttccga aggcaagcac gacgagggcc gcggcgagac cgaggacctg
     1921 ctcgccgtcg ccggctacct gaagcggcag tacggcggtc tccccctcgt gctcgccggt
     1981 ttctctttcg gcagcttcgt gcagacacgc gttgccaagc gcctgaaacc ggagcgcatg
     2041 gtcctggtgg gcgccgcggt caaccgtttt cacgcggaaa ccgtgccgca tgacaccctg
     2101 gtgatccacg gcgagcgcga cgacgtcgtg ccgttgcaga cggtgctcga ctgggcgcgt
     2161 ccccagggcc tgccggtcgt cgtggtgccg ggcggcgagc atttcttcca cggccgtctc
     2221 aacctgctcg ccgacgccgt cgtgcgtttt ttccgcggct gacaccgctg aaaaaacgaa
     2281 tggataaacc ctgaatgagc ctcgaaaacg ctttctacct gcgcgtgagc ggattgcgca
     2341 agagctatgc cggccaggaa gtcgtggccg gcatagacct cgagctcaag cccggtgaat
     2401 gtttcggcct gctcggcccg aacggcgcgg gcaagaccac gacgctgcgc ctgtgcctcg
     2461 ggctgatcga tcccgacggc gggaacatcg aggtgctggg gctgccggtt ccggcgcggg
     2521 cgcgcgaagc acgccgccgc atcggcgtcg tgccgcaggc ggacaatctc gaccccgatt
     2581 tcaccgtgga ggagaacctg ctggtgtacg gccgttattt cggcctggac gaagccgaag
     2641 cccgcggccg catccccgcc ctgctcgatt tcgcgggctt gagcgcgcgc gcgagctcgc
     2701 gcatccagac gctctccggc ggcatgaaac gacggctcac gctcgcccgc gccctcatca
     2761 acgatcccga cctgatcttc ctggacgagc ccaccaccgg cctcgacccg caggcgcgtc
     2821 acttgatctg ggaacggctg cgccatctgc tcgcccgggg caagacgatc ttcctcacca
     2881 cccatttcat ggacgaggcg gagcggctcg cccaccggct cgccatcatg gaccgcggcc
     2941 gcatcatcgc gctcggggcg ccgcgcgagc tgatcgtccg ccacatcgaa ccgcaggtag
     3001 tggaggtgta cggggacggc gccgagcgct gggcccgtga attcggccgg gcgcggtccg
     3061 agcgctgcga gcaggcgggc gagaccgtgt tctgttacgc gcgcgacgcc gggccgctgg
     3121 tccagaacct ggagagccag gaggagctgc gttacctgca ccgccacgcc aatcttgagg
     3181 acgtctttct caagctcacc gggcgcgaac tgagggacta gtgattgagt gactaggtga
     3241 ttaagtaact gagtcattca acctccgatc accgcaatcg gctcttcctg atcacttaat
     3301 cacctaatca cttagtcact aagtcacttg catcctttgc gatggagtat gcaaccatga
     3361 tcacgaaaac gctggcacta cctgctttca gtttgcggca cctgcacgtc tggcgccgca
     3421 attcgctggt ctggcgcaag cttgccattc cctcgatgct cggcaacctc gccgaccccc
     3481 tgatctacat gctggggctg ggctacgggc tcggcgcgat gctctccgac gtcggcggca
     3541 tgtcctacat cgcgttcctc gccaccggca tcgtgtgctc gagcaccatg atgagcgcct
     3601 cgttcgaggc gatgtactcg ggcttctcgc gcatgcacgt gcagaaaacc tgggacgcga
     3661 tcatgaacgc gccggtgacg ctggacgacg tggtgctggg cgaagcggta tgggcggcga
     3721 gcaagagcct gctttccgga atggcggtgc tgctggtggc gtgggcgatc gggctcgtgc
     3781 actcgggcag cgccttgtgg gcgatacccg tcgtgttcct gacggggctg gccttcgcca
     3841 gcctgggcct cgtcatgacg gcgctctccc ccagctacga ctttttcatg tactacttca
     3901 cgctcgcgat cacgcccatg atgctggtgt ccggggtgtt cttccccgtg tcgcaactgc
     3961 ccgggccgtt gcaggcggtg acggcgttcc tgcccctgac ccatgccgtg gagctggtgc
     4021 ggccgctgat gagcggtgta acgccggcgg cgatccccct gcatctttcc gtgctcgccg
     4081 cctatgccgt ggc
//
LOCUS       DBNGWZ010000657         2213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3139257, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000657 DBNGWZ010000000
VERSION     DBNGWZ010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3139257"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16CX_20520"
     CDS             complement(<1..458)
                     /locus_tag="AC16CX_20520"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR3014925.1"
                     /translation="MTPDSSNLEQKRGGLRSDQLSGLLLLALALYVGWENRAYPIGSL
                     HEPGPGYMPLALAIFLGAMGLLTVIFGGGSTQLSRVRWPELGRAIVVLVTCGVGAFAL
                     EHLGYRLTILAMLIFFVGVVERRHWLAAAAVSAGFSFISYFVFATWLRVPL"
     gene            complement(555..1535)
                     /locus_tag="AC16CX_20525"
     CDS             complement(555..1535)
                     /locus_tag="AC16CX_20525"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014926.1"
                     /translation="MKTRGWIAVSLWALAVAPVFAQEAFPGRGIAMIVAFPPGGVADI
                     TARPTAAAMEKVLKQPVSVVNRPGAAGAVGNATVANAKPDGYTILMALSSISVIPAAD
                     ELFNRKPAYSLDQFAPIALISADPTILVVHPSLPVKSLKELVALARSKPGQLSFSSSG
                     IYGALHMPMEMFLHATKLKMRHLPTSGGGPAITALLGGHVELTAGGPAAISGHVKAGK
                     MRTIVGWGAERHPSFPDVPTFKELGYKDVEYYIWAGMFAPKGTPEPVMKVLRDSARRA
                     VEDPDFKNAMAKVSSLVQYKDAPEFQKYWLADAKRLAALVKIVGKVEDKK"
     gene            complement(1613..>2213)
                     /locus_tag="AC16CX_20530"
     CDS             complement(1613..>2213)
                     /locus_tag="AC16CX_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3014927.1"
                     /translation="SSGVDATGKEVVPEVPILFTKWPNSVIGPGEPIPSANDYTDTTD
                     YEGELAVVMGEGGRNIAGKDAYDHVYGYTIVNDVSARTIQNRHRQWFLGKSPDGYCPM
                     GPCILTADEVKEIGHLQLITKVNGELRQNAFVSQLIFDIPTLIETLSRVMTLEPGDLI
                     ATGTCAGVGIGFDPPRYLKKGDVVSITIEPIGTLENPVG"
BASE COUNT          386 a          727 c          691 g          409 t
ORIGIN      
        1 agcggcaccc tgagccaggt cgcgaacacg aaataggaaa tgaaggagaa cccggccgag
       61 accgccgccg cggcgagcca gtgcctgcgc tcgacgaccc ccacgaaaaa gatgagcatc
      121 gcgagtatcg tcagacggta acccagatgc tcgagcgcaa acgcgccgac accgcaggtg
      181 acgagaacga cgattgcgcg acccagctcc ggccagcgca cacgcgagag ctgcgtgctc
      241 ccgccgccga agatcacggt cagcagcccc atcgcgccga ggaaaattgc gagcgccagc
      301 ggcatatagc cggggcccgg ttcgtgcagc gagccgatcg ggtaggcccg gttctcccag
      361 cccacgtaga gggcgagcgc gagcagcaat aggccggaga gctggtcgct ccgcaatccg
      421 cctcgcttct gttcaaggtt cgatgagtca ggagtcatct cgattgctcg ccggagcatt
      481 cagcggtcag ctatcagcag ccggcttgag acgttttttg ccttggctga gagctgatcg
      541 ctgatggctg atcgctactt cttgtcctcc accttgccca cgattttcac cagcgcggcg
      601 aggcgcttgg cgtcggccag ccagtacttc tggaactccg gcgcgtcctt gtactgcacg
      661 agcgagctta ccttggccat ggcgttcttg aagtccgggt cctccaccgc cctgcgcgcc
      721 gagtcgcgca gcactttcat caccggctcg ggggtgccct tcggcgcgaa catgccggcc
      781 cagatgtagt actcgacgtc cttgtatccg agctccttga acgtcggcac gtcgggaaag
      841 gagggatgcc gctcggcgcc ccagccgacg atcgtgcgca tcttgccggc cttcacgtgg
      901 ccgctgatcg ccgccggacc gcccgcggtc agctcgacgt gcccgcccag cagcgcggtg
      961 atggcgggac cgccgccaga agtcggcaga tgccgcatct tcagtttggt cgcatgcaag
     1021 aacatctcca ttggcatgtg caacgcgccg tagatgccag aagacgagaa cgatagctgc
     1081 cctggcttcg acctcgcgag cgcaaccagc tccttgaggg atttcaccgg cagcgacgga
     1141 tgcaccacga ggatggtcgg atccgcggag atcaatgcga tcggcgcgaa ctgatcgagc
     1201 gaatacgccg gcttgcggtt gaacagctcg tcggccgccg gtatcacgga gatgctcgac
     1261 agcgccatca ggatcgtgta gccgtcgggc ttggcgttcg ccacggttgc gttgccgacc
     1321 gcgccggcgg cgcccggccg gttcacgacc gagaccggct gcttgagcac tttctccatc
     1381 gcggccgcgg tcggcctggc cgtgatgtcg gccaccccgc ccggcgggaa cgccacgatc
     1441 atcgcgatac cgcggcccgg aaacgcttcc tgagcgaaaa ccggcgcaac cgccagcgcc
     1501 cacagcgata ctgcgatcca tcctctggtt ttcatggtcc ctcctccttg tttttcatca
     1561 ggtggattct acaggcgaac ccgcttccta tccgcttttc ctagcggctc attcaaccga
     1621 cagggttttc cagcgtgccg atgggctcga tggtgatgga aacgacatcg cctttcttca
     1681 ggtatctcgg cggatcgaag ccgatgccga cgccggcgca ggtgccggtg gcgatgagat
     1741 cgccgggctc gagcgtcatc acgcgcgaca gcgtctcgat cagcgtcggg atatcgaaga
     1801 tcagctggct cacgaacgcg ttctggcgca gctcgccgtt gaccttggtg atgagttgca
     1861 gatggccgat ttccttcact tcatcagcgg tgaggatgca cggtcccatc gggcagtacc
     1921 catctgggct cttgccgagg aaccactgcc ggtgccggtt ctggatcgtg cgcgcggaga
     1981 cgtcgttgac tatggtatag ccgtagacgt gatcgtaggc gtccttcccc gcgatgttgc
     2041 ggccgccctc gcccatcacc accgccagct cgccttcgta gtcggtggta tcggtgtagt
     2101 cgttggcgct ggggatgggc tcgcccgggc cgatcaccga gttcggccac ttggtgaaga
     2161 ggatcggcac ctccggcacc acctctttgc cggtcgcgtc cactccgctc gaa
//
LOCUS       DBNGWZ010000658         4106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3390353, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000658 DBNGWZ010000000
VERSION     DBNGWZ010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3390353"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="AC16CX_20535"
     CDS             <1..1435
                     /locus_tag="AC16CX_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014928.1"
                     /translation="FPWRSMPWVARRTAIARFCMSRFIRVSAVAFAAAPAEAAAHAFG
                     ARYDLPLPLSLWLTAAGLTVALSFLVFALALRRGVSSAAYPRLALLRLPPLAVEALRA
                     VSVCVLFVLIATGFAGTQDPYRNLAPAFIWIAWWIGFTYLSALVGDFWAVLNPWKALY
                     PAGHFGLRRELPAAVGVWPATALLLAFAWIEIVWEGNAVPANIAWLAIGYSALTWTGM
                     FVFGREAWLASGEVFAVYFNWFAKLAPSELRTKNRAACEWNLRPPAAGLLDPRPVPPS
                     AAFFVIVMLATVTFDGLRETPLWAVSDSLWAPAATVGLLALPCVFALVIVGTCTLMAW
                     ITRADARPPSAGELARLFVPTLLPIAFAYHVAHYLSYLLVAGQVVIPLASDPLGRGWD
                     LFGTAHYKIAVGVVGARFAWYTSVVVIVAGHVLAMYVAHRLALGRFSERRTARRSQYP
                     LAVLMVAYTMVSLWILAQPIVETGPGR"
     gene            1471..2145
                     /locus_tag="AC16CX_20540"
     CDS             1471..2145
                     /locus_tag="AC16CX_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014929.1"
                     /translation="MRWAGEVLRGMATALAAHPGVFASVTLAVAALNVLAPVAILSAV
                     RKPLDFFTFNPWLKRLPEYLGSDDAAIGEKLQKLPDLALFWFSAGGTYGGTEWGFAVD
                     VADLGRILLVSALFGLYFALWRRHRELVPRGVRADAALRRGGVAGAALTVFGISTGAC
                     SVTGCGAPVLPVIGLAFVGLESGTLHFLAQSSRVGTIALFAVLFLAVGYLGWKTGRAQ
                     DRLPRR"
     gene            complement(2208..3584)
                     /gene="radA"
                     /locus_tag="AC16CX_20545"
     CDS             complement(2208..3584)
                     /gene="radA"
                     /locus_tag="AC16CX_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3014930.1"
                     /translation="MAKAKSVFSCTECGGQALKWQGQCPHCQAWNTLVETVAEAAPTA
                     NRFGLAAEAGKLQRLSEVEAREESRIPTGIEEFDRVLGGGLVPGGVVLLGGDPGIGKS
                     TLLLQSLAGIGGARKVLYVTGEESTEQVALRARRLALDARGVHVLPEINLEKIQAALT
                     AEKPEIAVIDSIQTLYSGQLQSAPGSVAQVRECAAQLTRVAKSGGTAIVLIGHVTKEG
                     ALAGPRVLEHMVDTVLYFEGDTHSSFRLIRAFKNRYGAVNELGVFAMTEKGLKGVSNP
                     SALFLSQHGSEVAGSCVMVTQEGTRPMLVEIQALVDEAHAPNPRRLSVGLEQNRLALL
                     LAVLHRHAGIACFDQDVFVNAVGGVRISEPAADLAILMAVVSSLKNKPLPARLIVFGE
                     VGLAGEVRPVQRGQERLREAAKLGFTQALIPHANRPRQPIAGIEVMPVRRIEEAVIRV
                     RDLVSKSP"
     gene            complement(3740..>4106)
                     /locus_tag="AC16CX_20550"
     CDS             complement(3740..>4106)
                     /locus_tag="AC16CX_20550"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR3014931.1"
                     /translation="QRYAVAIGAVVLAFALRYGIYGTLDNRLPFTFFTTATVVAAWYG
                     GLGPGLLAALSGLLLGDYFFLPPHRAWGPLGEPERTAIGSYALTNALIVVLFWRLHMR
                     LGDLKDKLRKLTGGPDPSS"
BASE COUNT          562 a         1426 c         1352 g          766 t
ORIGIN      
        1 tttcccgtgg cgatccatgc cgtgggtggc tcgacgcacc gccatagcgc gcttctgcat
       61 gtcgaggttc atccgcgtta gcgcagtggc gttcgcggct gcgcccgccg aggcggcggc
      121 gcatgccttc ggcgcgcgct acgacctgcc gcttccgctt tccctgtggc tcaccgccgc
      181 ggggctcacc gtcgcgcttt ccttcctggt ctttgcgctc gcgctgcggc gcggcgtcag
      241 cagcgctgcc tatccgcggc tggctctgct gcgcctgcct ccgcttgcgg tcgaggcgct
      301 acgcgccgta tcggtgtgcg ttcttttcgt tcttatcgcg accgggttcg cgggtactca
      361 ggatccctac cgcaatctcg cgcccgcttt catctggatc gcgtggtgga tcgggttcac
      421 ctatctcagc gcgctggtgg gcgatttctg ggcagtgctc aacccctgga aggcgctcta
      481 ccctgcgggg catttcgggc tgcgccgcga gcttccagcc gcagtcggtg tatggccggc
      541 gacggcgctc ctgcttgcct tcgcctggat cgaaatcgtg tgggaaggca acgcggttcc
      601 agccaatatc gcctggctcg cgatcggcta ttcggctctc acctggacag gaatgttcgt
      661 tttcggccgc gaagcgtggc tagcgagcgg cgaagtcttc gcggtgtact tcaactggtt
      721 cgccaagctc gcgccgagcg aattgcgaac gaagaaccgc gccgcctgcg agtggaacct
      781 gcgcccgccc gcggcggggc tgctcgatcc gcggccggtc ccgccttccg ccgcgttctt
      841 cgtaatcgtc atgctcgcca ccgtcacctt cgacggcctg cgcgagacac cgctctgggc
      901 ggtcagcgat tcgctttggg cgccggcggc gacggtcgga ttgctggcgc ttccttgcgt
      961 gttcgcactg gtcatcgtag gtacctgcac cttgatggcg tggatcacgc gtgcggatgc
     1021 gcgtccccct tcggcggggg agctcgcgcg cctgttcgtg cctaccctgc tgcccatcgc
     1081 cttcgcctac cacgtcgcgc attacctttc ctatctgctg gtggcgggcc aggtggtgat
     1141 cccgctcgcc tcggacccgc tcggccgcgg ctgggacctg ttcggcaccg cccactacaa
     1201 gattgcggtg ggcgtcgtcg gcgcacgatt tgcctggtac acatcggtcg tcgtcattgt
     1261 ggccggccac gtgctcgcca tgtatgtcgc acaccgccta gcactcgggc gcttttccga
     1321 gcggcgcacc gcgcggcgca gccaatatcc gctcgccgtg ctgatggttg cctacaccat
     1381 ggtgagcctc tggatcctcg cgcagccgat cgtcgagacc ggcccgggcc ggtaaaatga
     1441 cagcccgttc cagcttggaa gtcggcacct atgcgctggg caggcgaggt actgcgggga
     1501 atggccacgg cgttggccgc gcatcccggc gtgttcgcgt cggtgacgct cgccgtcgcc
     1561 gcgctcaacg tgctcgcgcc ggtcgcgatc ctgtcggcgg tgcgcaagcc gctcgatttc
     1621 ttcaccttca acccgtggct gaaacgcctc cctgaatacc tcgggtccga tgacgcggca
     1681 atcggggaga agttgcagaa gcttcccgat cttgcgctct tctggttctc cgcgggcggc
     1741 acttacggtg gcaccgagtg gggtttcgcc gtggacgttg ccgacctcgg gcgcattctc
     1801 ctcgtctccg cgctgttcgg gctgtacttc gccctctggc gccgccatcg cgagctggtt
     1861 ccccgtggcg tgcgggccga cgcggcgctg cgccgtggcg gtgtcgccgg cgccgccctg
     1921 accgtgtttg gcatctccac cggcgcgtgc agcgtcacgg gctgcggggc gccggtgctt
     1981 cccgtgatcg gcctggcatt cgttgggctc gagagcggca ccctgcactt tctcgcccag
     2041 tcgtcgcgcg tcgggacgat cgcgcttttc gccgtgcttt tcctcgccgt cggctatctc
     2101 gggtggaaaa cgggccgcgc gcaggatcgc ctaccgaggc ggtagcggct tgacgcgcca
     2161 attcgaatcc agacgataca cgcgtcgcgc gtcatagcaa ccgtccgtca aggactcttg
     2221 gataccaggt cacgcacgcg gatcaccgcc tcctcgatgc gccgcaccgg catcacctcg
     2281 atgcctgcaa tcggctgtct cgggcggttg gcgtgcggga tcagcgcttg cgtgaatccg
     2341 agcttggccg cctcgcgcag ccgctcctgt ccgcgctgca ccggccgcac ctcgccggcg
     2401 agccccactt ccccgaacac gatgagcctg gcgggcagtg gtttgttttt cagcgatgac
     2461 accaccgcca tcaggatcgc gagatcggcg gcgggctcgg agatcctcac gccgccgacg
     2521 gcattcacga acacatcctg gtcgaagcag gcaatgccgg cgtgccggtg cagcacggcg
     2581 agcagcagcg cgaggcgatt ctgctcgagc ccgaccgaga gccggcgcgg attgggggcg
     2641 tgcgcctcat ccaccagcgc ctggatctcg accagcatcg ggcgcgtgcc ttcctgcgtc
     2701 accatcacgc acgagcccgc gacctccgac ccgtgctgcg acaggaacag cgccgagggg
     2761 ttggacacgc ccttcaggcc cttctccgtc atggcgaaca cgcccagctc gttgaccgcg
     2821 ccgtagcggt tcttgaaggc gcggatgagg cgaaagctcg aatgcgtgtc gccctcgaaa
     2881 tagagcaccg tgtccaccat gtgctcgagc acgcgcgggc cggcgagcgc tccttccttg
     2941 gtgacgtggc cgatcagaac gatcgcggtg cccccgcttt tcgcgacgcg ggtgagctgc
     3001 gccgcgcact cgcgcacctg cgcgaccgag ccgggcgccg actgcaactg tcccgagtaa
     3061 agcgtctgga tcgagtcgat aaccgcgatt tccggtttct ccgccgtcag cgccgcctgg
     3121 atcttctcga ggttgatctc gggcagcaca tgcacgccgc gcgcatcgag tgcgagccgg
     3181 cgcgcgcgca gtgcgacctg ctcggtggac tcttctccgg tcacatacag gaccttgcgc
     3241 gcgcctccga tcccggcgag cgactgcagc agcaaagtgg acttgccgat gccgggatcg
     3301 ccgccgagga gcaccacccc gcccggcacg agcccgccgc ccagcacccg gtcgaactcc
     3361 tcgatgccgg tggggatgcg ggattcctcg cgcgcctcga cctcggaaag acgctgcagc
     3421 ttccctgctt ccgcggccag accgaagcgg ttggcggtgg gcgcggcttc cgcgacggtc
     3481 tcgaccagcg tgttccaggc ctggcagtgc gggcactggc cctgccactt gagcgcctgg
     3541 ccgccgcact ccgtgcagga aaaaactgac ttggccttgg ccatttgcgg cccgcggttc
     3601 gtggttcgtg gttcgtggct cgtggctcgt ggctcgtggc ccgatgcagg gtatcaacgc
     3661 gcgggccgcc tgtcacgcgt acgcagccgt tgaagacacg ccgcgttacg aatcccggcg
     3721 ggcgccgggg cacccggtct taactggacg ggtccgggcc gcccgtgagc ttccgcagct
     3781 tgtccttgag gtcgcccagg cgcatatgca atcgccagaa cagcaccacg atcagcgcat
     3841 tggtcaaggc atagcttccg atcgcggtgc gttccggttc gccgagcggt ccccaggccc
     3901 gatgcggcgg aaggaagaaa taatcgccga gcagcagacc ggacagcgcg gccagcaatc
     3961 cggggccgag tcctccgtac caagcggcga ccacggtcgc tgtggtgaag aaggtgaacg
     4021 ggagccggtt gtcgagcgtc ccgtagatgc cgtagcgcag cgcgaacgcg agcacgaccg
     4081 cgccgatggc aacggcatag cgctgg
//
LOCUS       DBNGWZ010000659         3704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_117855, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000659 DBNGWZ010000000
VERSION     DBNGWZ010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_117855"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC16CX_20555"
     CDS             <1..368
                     /locus_tag="AC16CX_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HIR3014932.1"
                     /translation="RGCRLRPCWFEPTFHKHAGKPCAGLQIHVEDAAYDHGVFQPWRL
                     LALAFKALRTLAPEYPLWRDFPYEYEYDRRAIDLINGSGLLREWVDDRAAVASDLEAL
                     AVPDERAWREERESLLLYR"
     gene            complement(467..1738)
                     /locus_tag="AC16CX_20560"
     CDS             complement(467..1738)
                     /locus_tag="AC16CX_20560"
                     /inference="COORDINATES: protein motif:HMM:NF039309.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR3014933.1"
                     /translation="MADSRLWRLAAALLLPFIGVVAAFGIAPDTVTDPIARTQVVQDI
                     ALPPTRPTGDLEEGYWREERIQRGDTFAALLARLGAEDPGAAEFLRNTRSARTLHQLI
                     PGRTVRALTTAEGRLLALRYQNGGKVLNVRRQDGTFRLTEEPVELERRVLMKSGEITS
                     SLFAATDAAGLSDAVAIQIADVFSTDIDFHRDLRRGDHFSVVYEMFYDQGEPVRSGRV
                     LAAEFINVGRLYRAVYFQYAEGQGGYYTPDGRNIRKAFLRSPLEFSRISSGFANARFH
                     PILRQWRAHKGIDYAAPTGTRVKATGDGVVEFAGRHPGGYGNLVVLRHQSKFTTWYGH
                     LSGFAKGMRKGARVAQGETVGYVGATGLATGPHLHYEFRINDVHQNPLRVVLPSAPPI
                     GPEEKPAFDAAAAPLAQRLQLLRVTNLARLD"
     gene            1908..3110
                     /gene="tyrS"
                     /locus_tag="AC16CX_20565"
     CDS             1908..3110
                     /gene="tyrS"
                     /locus_tag="AC16CX_20565"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR3014934.1"
                     /translation="MKPAVEQQLEIIKRGCDELLVEAELRERLKSGRPLRVKAGFDPT
                     APDLHLGHTVLINKLRQLQDLGHHILFLIGDFTGMIGDPAGRNATRPPLSREEVQRNA
                     ATYTDQVFGILDRERTEVVFNSAWMDRVNAADIIRLAAKHTVARMLERDDFAKRYKGK
                     QPIAIHEFLYPLVQGYDSVQIKADLELGGTDQKFNLLMGREVQKEHGQPPQVILTLPL
                     LEGLDGVSKMSKSLGNYVGITEPPGEIFGKLMSISDDLMWRYIELLSFESPQKIKEKK
                     KAVEQGGNPRDVKFDFAKEIVARFHGTPAAEAAAAEFEARFKRDEIPAGIPEVSIAAK
                     GDALSISQVLKQAGLTASTSEALRLIEQGGVKLNGERVNDKAQKLARGTRSVLQVGKR
                     KFVRVTIE"
     gene            complement(3156..>3704)
                     /locus_tag="AC16CX_20570"
     CDS             complement(3156..>3704)
                     /locus_tag="AC16CX_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014935.1"
                     /translation="PRFRQGDYDGGVTQGAGAIIAQLEGRPGQAASDAPQQAQKSEFI
                     KAPEMALHEKVLMGAFVFGIIGLFTFIGIVTPGFGWFLYFFLIPFWAMFPAVIFGSNI
                     AFYILIAYLTGFPIAKRLMSRSEMYRKAMEDIKKKGHASIGGFTIGSSSGGSSWSSSS
                     SSSGGFSGGGGSSGGGGASGSW"
BASE COUNT          692 a         1194 c         1214 g          604 t
ORIGIN      
        1 tgcgcggatg ccgcttgcga ccgtgctggt tcgagcccac gttccacaag cacgccggga
       61 agccatgcgc cgggctgcaa atccatgtcg aagatgcggc ctacgatcac ggcgtcttcc
      121 agccctggcg cctgctcgcc cttgctttca aggcactgcg gacgctcgct cccgagtatc
      181 cactgtggcg cgatttcccg tacgaatacg agtacgatcg gcgcgccatc gatttgatca
      241 acggcagcgg cttgctgcgt gaatgggtgg atgatcgcgc agcggtcgcg tccgatctgg
      301 aagcgctcgc cgttcccgac gagcgggcct ggcgcgagga gcgcgaatcc ctgctgctct
      361 accgctgaat cggaggaaag tgagcggtga gccgtaagcg gtaagccgtg ggatcccgta
      421 caggcgtgtt ttaccgatta ccgcttaccg cttactgctt actgtattaa tccagccggg
      481 cgagattggt gacgcgcagg agctgcaggc gctgcgcgag cggcgccgcg gcagcgtcga
      541 acgccggctt ctcctcggga ccgatgggcg gggcggaggg cagcaccacc cgcagcgggt
      601 tctggtgcac gtcgttgatg cggaattcgt agtgcaggtg cggcccggtg gcaagccccg
      661 tcgccccgac gtaaccgacc gtttcgccct gggcgacgcg ggcgcccttg cgcatgccct
      721 tggcgaagcc ggagagatgg ccgtaccagg tggtgaattt cgactgatgg cgcagcacca
      781 ccaggttgcc gtagccgccc gggtggcgtc ccgcgaattc gaccacgccg tcgccggtgg
      841 ccttcacgcg ggtgccggtg ggggcggcgt agtctatgcc tttgtgcgcg cgccactgcc
      901 tgagtatcgg atgaaagcgc gcgttggcga agccggagga gatgcgcgag aactcgagcg
      961 gcgagcgcag gaacgccttg cggatgttcc tgccgtccgg cgtgtaatag ccgccctggc
     1021 cctcggcgta ttggaaataa accgcccggt acagcctgcc gacgttgatg aactccgcgg
     1081 cgagcacccg tcccgaccgg accggctcgc cctggtcgta gaacatctcg tagaccaccg
     1141 agaagtggtc tccgcgccgc aggtcgcggt ggaaatcgat gtcggtggag aacacatcgg
     1201 cgatctggat ggcgactgcg tcggagagac cggcggcgtc ggtcgcggcg aagagcgagc
     1261 tggtgatctc gccggacttc atcagcacgc gccgctccag ctcgaccggc tcctcggtca
     1321 agcggaaggt gccgtcctgg cgccgcacgt tgagcacctt gccgccgttc tggtagcgca
     1381 gtgcgagcag cctgccttct gcggtggtga gcgcgcgcac cgtccggccg ggaatcagct
     1441 gatgaagcgt cctggccgaa cgcgtgttgc gcaggaattc ggccgcgccg ggatcctcgg
     1501 cgccgagccg cgcgagcagc gccgcgaacg tgtccccgcg ctgaatgcgc tcctcgcgcc
     1561 agtagccttc ctccaggtcg ccggtgggtc gtgtcggcgg cagcgcgatg tcctgtacta
     1621 cctgggttct tgcgatcgga tcggtgacgg tgtcgggcgc gatgccgaaa gcggccacca
     1681 cgccgatgaa gggaagcagc agcgccgcgg ccagccgcca gagcctggaa tccgccatga
     1741 gccgcgcgag ctttagacgc caggtccgac acactgctaa aatcacgacg tatccctgcc
     1801 gcatgatttc cctgatattt tcgggagcgg caagcttagc agaattgccc atgccggcaa
     1861 acccgatttt tgcatcgtga aatagtgcac accactgcct ggacagcgtg aagcccgccg
     1921 tcgaacaaca actcgagatc atcaagcgcg gctgcgacga actgctggtc gaggcggaat
     1981 tgagggagcg gttgaaatcg ggccgcccgc tgcgcgtcaa ggccggcttc gatcccaccg
     2041 cccccgacct gcacctcggc cacacggtgc tcatcaacaa attgcggcag ctgcaggacc
     2101 tcgggcacca catcctgttc ctgatcggtg atttcaccgg catgatcggc gacccggccg
     2161 gcaggaacgc gacccgcccc ccgctctcgc gcgaggaggt gcagcggaac gccgcaacct
     2221 acaccgacca ggtgttcggg atactcgacc gggagcgcac ggaggtcgta ttcaactcgg
     2281 catggatgga ccgcgtcaac gcggcggaca tcatccgcct cgcggcgaaa cacaccgtcg
     2341 cgcgcatgct cgagcgcgac gacttcgcca agcgctacaa gggcaagcag ccgatcgcga
     2401 tccacgagtt cctctatcca ctggtgcagg gctacgactc ggtgcagatc aaggccgacc
     2461 ttgagctcgg gggcaccgat cagaaattca acctgctgat ggggcgggaa gtccagaagg
     2521 agcacgggca gccgccgcag gtgatcctca ccctgccgct gctcgaaggg ctcgacggcg
     2581 tcagcaagat gtcgaaatct ctcggaaact acgtcggcat caccgagccg ccgggcgaga
     2641 tcttcggcaa gctgatgtcg atctcggacg acctgatgtg gcgctacatc gagctgctat
     2701 cttttgagtc gcctcaaaag ataaaggaaa agaagaaggc tgttgaacag ggcggaaacc
     2761 cgcgcgacgt gaaattcgat tttgccaagg aaatcgtcgc gcgcttccac gggaccccgg
     2821 cggcggaagc ggctgccgcc gagttcgagg cccgcttcaa gcgcgacgaa atccccgccg
     2881 gcattcccga ggtcagcatc gcagcgaaag gtgacgcgct ttccatctcc caggtgctca
     2941 agcaggcggg actcacggcc agcacctcgg aagcgctgcg cctgatcgag caagggggcg
     3001 tgaagctgaa cggcgagagg gtgaacgaca aggcgcaaaa gctcgccagg ggaacgcggt
     3061 cggtgcttca ggtcggcaag cgcaagttcg tccgcgtcac cattgagtga accccggcac
     3121 gcggcaggcc gccacgaaac ggcggcgggc gtgtattacc agctgccgga tgcgccgccg
     3181 cccccggagc tgcccccgcc cccggaaaac cccccgctgg agctgctact tgagctccac
     3241 gacgagccgc cgctggagga gccgatggtg aacccgccaa tcgacgcatg gcccttcttc
     3301 ttgatgtcct ccatcgcctt tcgatacatc tccgaacgcg acatcaggcg cttggcgatg
     3361 gggaagcctg tcaagtaagc gatcaggatg tagaaagcga tattgctgcc gaagattacg
     3421 gccgggaaca tcgcccagaa cgggatcagg aaaaagtaca ggaaccagcc aaagcccggc
     3481 gtgacgatgc cgatgaaggt gaaaagcccg atgatgccga agacgaaggc gcccatcagc
     3541 accttctcgt gcagcgccat ctcgggggcc ttgatgaact ccgatttctg cgcctgctgc
     3601 ggggcgtcgg atgcggcctg ccccggccgc ccctccagct gggcgatgat cgcgcctgcg
     3661 ccctgcgtca cgcccccgtc gtagtcgccc tgccggaagc gcgg
//
LOCUS       DBNGWZ010000660        12906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2760734, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000660 DBNGWZ010000000
VERSION     DBNGWZ010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2760734"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="AC16CX_20575"
     CDS             <1..539
                     /locus_tag="AC16CX_20575"
                     /inference="COORDINATES: protein motif:HMM:NF027650.7"
                     /note="Uup; in Escherichia coli this cytoplasmic protein
                     was shown to contain ATPase activity; mutations in this
                     gene affect RecA-independent excision of transposons and
                     affects Mu bacteriophage growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3014936.1"
                     /translation="LVMDEPTNDLDVETLELLEEVLLDYQGTLLLVSHDRAFLDHVVT
                     STLVCEGGGRIGEYVGGYTDWLRSQQNRLASTPEPSPRVSTSAPAAMPSTEKLGAKSK
                     KLSFKEQRELNDLPKQIESLETEQAQLHARISEAGFYQQPSEAVTTTLARLESVSGEL
                     AACYARWEALEAPATATE"
     gene            683..925
                     /locus_tag="AC16CX_20580"
     CDS             683..925
                     /locus_tag="AC16CX_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HIR3014937.1"
                     /translation="MERAARDARFRRRLLIEAINQLLTGDLAAGKAILRDYINATISF
                     ERLARQTNKPSKSLYRMPGPHGNSTAENLFGIIKLF"
     gene            1230..2765
                     /locus_tag="AC16CX_20585"
     CDS             1230..2765
                     /locus_tag="AC16CX_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HIR3014938.1"
                     /translation="MTIAAESTDRARAELFRHVTVDKAALYRAIMKAFAAAKRQFRLH
                     LRPDEVRIEAQWPGAPPVLEEIQQALAQLVEWSNLQSQPDTARVSTLEDFYRARFLYS
                     LTSGGEAVETGLAAFAQALARRGELQSVALEDIVGRVSALRALADASPIDAAKVHETL
                     RDLVTVFNGLAENAQAFMAGLARSIELQRADLQAVMAYKARLIDYLERFIGDLVTRSA
                     RIGRELQDLAPDAERLLRSVAEREARDAAPDDETVEAKIQEERLQAWRERWNGLKGWF
                     VGDGRLPAQSELLRSRARAAIPRLLAAVAALNERRSGLSDRSADFRVLARWFAETESD
                     AEAHRLWRAAFAINPARHLSLASPDEDVPATTPWAVAPAIEIAPRLRERGQFAPRGAP
                     PRVRDRSRERELLAQRLAEENAQVHAARARLANGKAARLSELGRLDQHEFRLFLALLG
                     EALSVQGRPDRTVERVTADGLLRIRLEPLSVHTRAQIDTELGRFSGRDHVVTITALDR
                     AAS"
     gene            2762..3979
                     /locus_tag="AC16CX_20590"
     CDS             2762..3979
                     /locus_tag="AC16CX_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HIR3014939.1"
                     /translation="MSADRLADALTRHREYERERALRALLTRPLMTAADSDFGAVRRH
                     AEWLRDWLARETGWVLRVERDCARLFKLAADLKDETRGAPGFDRRRCMLLCLVCAALE
                     RAEAQITLRTLGERLIDLAADPELASRGFRFTLEPMHERRELVHVCRYLLGLGVLHRV
                     AGEEEGYVQHAGDALYDVQRRALAALLACTRGPSTFAPGSEPSSLVGRLAALTDEYVP
                     DSPEGRRTAVRHRLARRLLDDPVVYFDELSDEERDYLANQRGAMAARLRAASALRPEL
                     RAEGLALVDPDGELTDAALPAEGTVAHATLLVAQFLAEQGRAEPGRRTPEPEIAAFLR
                     RAADEYGRYWRKAERDPGAEIELAEEALARLAALKLVRRSHAGVEARPALARFALGQP
                     QFSASVQSGLPLS"
     gene            3976..8091
                     /locus_tag="AC16CX_20595"
     CDS             3976..8091
                     /locus_tag="AC16CX_20595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HIR3014940.1"
                     /translation="MTAPLPLSPLPEPGLTRWQPLRTGLIELYYYDCEEFWFRDGHLL
                     LRGNNGTGKSKVLSLTLPFLLDAQLGSARVEPDGDRGKRMDWNLLMGRHERRTGYAWI
                     EFGRIGEDGTPRYLTLGCGLSAVAGRARVDAWHFFTEQRIGRDLWLITPERVVLGRER
                     LAERLADRGQVFDTAQDYRRAVDERLFRLGEERYRALMDTLITLRQPQLSKRPDEENL
                     SNALTEALAPLPQATLEDVAEAMTQLDEFRDALAEHEALRDAVAQFDRRYRAYARVQA
                     RRQARVLRQAQTEFDNASRALGEARGELAAAEQTVGELKARCAELDRQLALDRAALDE
                     LRQDPVMRDARRLHETVHLAEERSRDAEEAGKRRDEAEGRHSEERAAAASRREESQQS
                     LARLTRDAGSAERLASEVGLASDHALIAAVLIPFADLGQRTPTQLEGLTSASREMVER
                     RRAQVAQIRHRLRETDDALRARDRAAEARADRAETFEAAQARARDADLRLKAAAEEYL
                     RQWGGFLDRLTTLALPDAQTAMETLSAWVESLAGENPMVALLARAQHEAARGFAEREA
                     ALKHRQAELRREADELVAERARLARGEDRAPPAPHTRAADVRRDRAGSPLWQLLEFRE
                     SVQESARAGLEAALEAAGLLDAWVTPEGAILDPDTQDVLLIPRAARASSLCDWLLPVE
                     GGTVPASRIAGILAGISCTLQDPGDAEAWVSPDGRFRLGPAQGAWRKPVAQYIGYAAR
                     VAARVRRLDEIAAELSALASELERVAADLRALDGSRRALNQECAHAPGDGALRNAHAK
                     FSADESQRREAHARLAEAEARCGDAERAWSRARETLQRDAEDLRLPADADGLSEVEGG
                     LLDYDRAALTVVQAAREHRRAVIETLEQERRERETQAQADTAREECARRVRAAEEAVA
                     RRDLLRETVGAAVAELEHRLAQKEKAVQSGEQILKGENKCLVEARERRARSGQKEEDA
                     QAFLRERQQRRQEAVEYLRAFAQTGLLSVALPELEAPDPAAAWTIDPALSAARRIEHL
                     LAEVPAEDTDWTRIQNDVSRDFTALTQAMSAQGQQAQAETSDYGLIVRIVYQNRPERP
                     DVLERRLGTEIRERREILTAREREVLENHLQAEVADGLQRLLQDAERRVQTINAELSR
                     RPTSTGVRFKLEWQPLPEGEDGAPAGLAEARKRLLNTAADAWSAEDRRLVGEFLQNRI
                     ASERSRDDGGALLDHLTRALDYRRWHRFRVKRFQDGAWRPLSGPASSGERALGLTVPL
                     FAAASSHYASAECPYAPRLVLLDEAFAGIDDEARAHCMALIREFDLDFVMTSEREWGC
                     YAELPGLAICHLVRREGVDAVFVSRWSWDGRARREEPDPSRRFPETAIASS"
     gene            8088..9290
                     /locus_tag="AC16CX_20600"
     CDS             8088..9290
                     /locus_tag="AC16CX_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 family protein"
                     /protein_id="HIR3014941.1"
                     /translation="MSTARERLERLLGGPALAGLRERLRRRYELGRNNDAFTLAGISP
                     QERRALEGLLGRRGRRSGSMRLTVSALDEALARAGLASSLRDALEQIDGPIRDLTAER
                     ADRLAQWNAVFAVCRNEPLAAFVAQSAACGLVKRLAASDPAQGRMLLDAASQVLARLP
                     AAGVPLSQLAAEVLCDAHALDEGRPVATLVLATLKSGDEEERSREVWANRGVLVNELA
                     APALVLNLPASADTAGGRLVAQARELGEPLHLSLRTLLRAPPQWEVTGRTVFVCENPA
                     VVAMAADRLSTFCAPLVCTDGMPAAAQRALLVQLAAAGVTLRYHGDFDWPGLTIGNFV
                     MRTFGAKPWRFGSEDYVATYGRRLQGAAVPAAWDAALGPRMAQHGYALDEEAVVEALL
                     HDLTFRQA"
     gene            9538..10008
                     /locus_tag="AC16CX_20605"
     CDS             9538..10008
                     /locus_tag="AC16CX_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014942.1"
                     /translation="MKTVSWKVAFAGLLGGFIGNGVLGALFSSPPIRHILYDPTIQSL
                     LFIEVTSQRNIPISVAGLVVLSVIHAWLFAVLFPALPGRTWVRKGAFWGLVIWLMYWV
                     FQEWFIYHTLLGEPWLLNLLELAVLLLGSVVEGVVIAFALARDARHVEPASVGR"
     gene            10258..11214
                     /locus_tag="AC16CX_20610"
     CDS             10258..11214
                     /locus_tag="AC16CX_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3014943.1"
                     /translation="MKNILITGATGQIGSELTPALRKRYGGGHVVAAGHTRHPDQDIA
                     ESGPYCSLDVRDLAALQTTVATHRIDTIVHLASVLSAAAEEKPQLAWDINVHGLMNVL
                     ETARLHHCAVFFPSSIAAFGPLTPAENTPQDTIQRPTTMYGITKVTGELLCDYYHARY
                     GVDTRGLRFPGLISSKTLPGGGTTDYAVHIFHAALKARQYECFLKPDTRLDMMYMPDA
                     INAAVQLMEADGRRLTHRNAYNVTAMSVAPRDIAEAIKQFIPDFTISYAIDPARQAIA
                     DSWPRHLDDRAAREEWGWRPAFDLTATVKDMLDTLSSRLKSA"
     gene            11313..12575
                     /locus_tag="AC16CX_20615"
     CDS             11313..12575
                     /locus_tag="AC16CX_20615"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3014944.1"
                     /translation="MSLTRFDALVTGKLEELEARGTLKGREHVITGVSAGEGGGSPRY
                     FLAGCGDRAFLRMNANSYLGLAFHRRVIEAHALATERFGAGPGAVRFISGTYLPHVEL
                     ERRLAAFHGRDAAMIMSAAYATVIGVLPQFVGEDTVVISDALNHNCIINAIRLSRPAK
                     KAVYAHRDMAALKHLLETHKGQVRRAVVVTDGVFSMRGDHAPLDDIAAVCARHEPGYA
                     EGIITVVDDSHGVGAFGATGRGTEEYTGTRADILIGTLGKAFGVNGGYVVSSAPVINY
                     LRETAPCYVYSNPITPAEAAAAVTALDILDSPEGRTLLEHMRRLAARLRAGLTDLGLE
                     TVRSEHPIVPILIRATRRTSALARYLFEKNILVTGLNYPVVPKGEEEIRLQVSANHTE
                     QDIESVLDAVKRFQEERGAESVNEYGSR"
BASE COUNT         2170 a         4144 c         4456 g         2136 t
ORIGIN      
        1 tgctggtgat ggatgagccc accaacgatc tcgatgtcga aacactggag ctgctggagg
       61 aagtgctgct cgattaccag ggcaccttgc tgctggtcag tcacgaccgc gcgtttctgg
      121 atcacgtagt cacgagcacg ctggtatgtg aaggcggggg gcggatcggg gaatacgtcg
      181 gcgggtatac cgactggctg cggtctcaac agaaccggct cgcatcaacg cccgaaccgt
      241 caccacgagt ctctacatca gccccggccg ccatgccatc cactgagaag ctgggcgcaa
      301 aatcaaagaa gctttccttt aaggaacagc gtgaactcaa tgatctaccg aagcaaatcg
      361 agtcgctaga gaccgagcag gcgcaactcc acgccaggat cagcgaggcg ggattctatc
      421 agcagccgag tgaggccgtg accacgacgt tggcgcgtct ggagtcggtg agcggggagc
      481 ttgcggcctg ctatgcccgt tgggaggcgc tggaggcgcc agcgacggcg acggagtgat
      541 caacgcgttc gtgctgggga agaagtaggt agttgggttc gtcaaacggc tgtggtaacg
      601 tacacgtcac ttgaataaga tcgccccaca tgaaacgggg atgacactga tgtcagtcac
      661 acgaacattt cgtatcacga tcatggagcg ggccgcccgc gacgcgcggt ttcgccggcg
      721 cctattgatc gaggcgatca accaattgct aacgggcgat ctggccgcgg gaaaagccat
      781 actgcgcgac tacatcaacg cgacgatctc ctttgagcgg ttggcgcgcc agaccaacaa
      841 gcccagcaag agcctctacc gcatgccggg gccgcatggc aattcgaccg cggaaaacct
      901 gttcggcatc atcaaacttt tttaggcgca tgagtgcgta cggctgcaag tcaaatagaa
      961 ccgatccgcc gcgtaataat ggcgcggcaa aagccggggc agtgctccgg agtccaacgc
     1021 gggcaagtgc tggaacagtt tggcgcgaac taatatagat tgcccaatgt tcttgctgag
     1081 gcgggggtgt cgctccgagt cgcaggggcc taagcaaata cctaaatcgt agagctggcc
     1141 cctcgaactt ggagggtgat ggcgccatgc tgattaagcg ggcatggcgg atccgagggg
     1201 tatatttcag gtgccgaaaa gggggatgtt tgacaatcgc tgccgaatcc acagacaggg
     1261 cgcgcgctga gctttttcga catgtcaccg tcgacaaggc ggcgctgtac cgcgccatta
     1321 tgaaggcgtt tgccgccgcc aagcggcagt ttcgtttgca cttgcggccg gacgaggtgc
     1381 gaattgaggc gcagtggccc ggcgcgccgc ccgtgctgga ggagatccag caggcactcg
     1441 cccagctcgt ggaatggagc aacctgcaat cgcagcccga cacggcgcgg gtttccacgc
     1501 tggaggactt ctaccgcgcg cgctttctct atagcttgac ctcgggcggc gaggccgtgg
     1561 agacgggact cgccgcgttc gctcaggcgc tcgcgcgccg cggcgagctg caatcggtgg
     1621 cactggagga catcgtcggt cgggtgtccg cgctgcgcgc gctcgccgat gccagcccga
     1681 tcgatgcggc gaaggtgcac gagacgctgc gcgacctcgt gacggttttc aacgggctgg
     1741 cggagaacgc gcaggctttc atggccgggc tggcacgcag catcgagctg cagcgcgccg
     1801 acttgcaggc tgtgatggcc tacaaggcgc gactcatcga ttaccttgag cgcttcatcg
     1861 gcgacctggt gacccgctcg gcgcgcatcg ggcgcgagct ccaggatctc gcgcccgatg
     1921 ccgagcgcct gctgcgctcg gtcgccgagc gcgaggcgcg cgatgcggcg cctgacgacg
     1981 aaaccgtcga ggcgaagata caggaggaac ggctgcaagc ctggcgcgag cgatggaacg
     2041 gtctcaaagg ctggttcgtc ggcgacggcc ggctgcccgc ccaatccgag ctgcttcgca
     2101 gccgggcgcg cgcggccatc ccgcggctgc tcgccgcagt cgcggcgctc aacgagcgcc
     2161 gcagcggtct cagcgaccgc tcggctgact tccgcgtgct ggcgcgctgg ttcgcagaga
     2221 ccgaatccga cgccgaggcg cacaggctgt ggcgcgcggc cttcgccatc aaccccgccc
     2281 gccacctgag ccttgcctcg ccggacgaag acgtgccggc gacgacgcct tgggccgtcg
     2341 ccccggcgat cgaaatcgcc ccgcggctgc gcgagcgcgg ccagtttgcg ccgcgcggcg
     2401 cgccgccgcg cgtgcgggac cgatcgcgtg agcgggaact gctcgcgcag cggcttgcgg
     2461 aggaaaacgc gcaggtacat gccgcccgtg cccggctagc gaacggcaaa gccgcacggc
     2521 tatccgagtt ggggcggctc gaccaacacg aatttcggct tttccttgcg ttgctcggcg
     2581 aagcgctttc cgtgcagggt cgcccggatc ggacggtcga acgcgtgacc gcggatggtc
     2641 tgctgcgcat ccggctcgag ccgctgtcgg tgcatacgcg ggcgcagatt gataccgagc
     2701 tcggccgctt ttccgggcga gaccacgtgg tgaccatcac tgccctggat cgagctgcct
     2761 catgagcgcc gatcggcttg ccgacgcatt gacccggcat cgcgagtacg agcgcgaacg
     2821 agcgctgcgt gcactcctca cgcggcctct catgaccgcg gcagactccg atttcggcgc
     2881 cgtgcgtcgg cacgcggagt ggctgcgcga ctggctcgcg cgcgagaccg gatgggtgct
     2941 gcgggtcgag cgcgactgcg cacgcctgtt caagcttgcc gccgatctca aagacgagac
     3001 gcgcggcgcg cccggcttcg accgccggcg ctgtatgctt ctgtgtctgg tgtgcgcggc
     3061 gctcgagcgc gccgaagcgc agatcacgtt gaggacgctc ggcgagcgcc tgatcgatct
     3121 tgccgccgat ccggagctgg cgtcgcgcgg ctttcgcttc acgctggagc cgatgcacga
     3181 gcggcgcgag ctggtccacg tctgccgcta tctcctcggg ctgggcgtgc tgcaccgcgt
     3241 ggcgggcgaa gaggaaggtt acgtgcagca cgcaggcgat gcgctctacg acgtgcagcg
     3301 gcgggccctg gccgcgttgc ttgcctgcac gcgcggacct tccaccttcg cgccgggctc
     3361 ggagccctcg agcctcgttg gtcggctcgc cgcgctgacc gacgagtacg tgccggacag
     3421 tccggagggc agacgcacgg ccgtgcgtca ccgcctggca aggcgcctgc tcgacgaccc
     3481 cgtggtctat ttcgacgagt tgtccgacga ggagcgcgac tacctcgcca atcaacgcgg
     3541 cgccatggcg gcgcgcttgc gcgcagcgag cgcactcagg ccggaactgc gtgcggaagg
     3601 tctcgcgctg gtcgatccgg acggcgagct aaccgacgcg gcgttgccgg ccgaaggcac
     3661 cgtagcccac gccactttgc tcgtggcgca gtttctggcc gagcagggca gggccgaacc
     3721 cgggcggcgg acgcccgagc cggagattgc cgccttcctg cggcgcgccg cggacgagta
     3781 tgggcgctac tggcgcaagg ccgagcgcga tcccggcgca gagatcgaac tcgccgagga
     3841 agcgctggcg cgccttgccg ccctcaagct cgtgcggcgt tcgcatgcgg gcgttgaggc
     3901 gcgccctgcg ctcgcacgct tcgccctggg ccagccgcag ttcagtgcat ccgtgcaatc
     3961 gggtttgccg ctctcatgac cgcgccgctg ccgttatctc ccttgcccga gcccgggctt
     4021 actcgatggc agccgctgcg cacggggtta atcgagctgt attactacga ctgcgaggag
     4081 ttctggtttc gcgacggcca tctgctgctg cgcgggaaca acggcacggg caagtccaag
     4141 gtgctgtcgc tcacgcttcc gttccttctc gacgctcagc tgggctccgc gcgcgtcgag
     4201 cccgacggcg accgcggcaa gcgcatggac tggaacctcc tgatggggcg gcacgagcgg
     4261 cgcaccggct acgcgtggat cgagttcgga cggatcggcg aggacggtac gccgcgttat
     4321 ctgactctgg gctgcggcct ttccgcggtc gcgggtcgcg cgcgggtcga cgcgtggcat
     4381 ttcttcacgg aacagcgcat cggccgggac ctatggctga tcacgccgga gcgtgtcgtg
     4441 ctcggccgcg aacggctcgc ggagcggctt gcggaccggg gccaagtctt cgacaccgcg
     4501 caggactacc ggcgcgcggt tgatgagcgc ctgtttcgcc tgggcgagga gcgctaccgc
     4561 gccctcatgg acacgctgat cacgttgcgc cagccgcagc tctcgaagcg gcccgacgag
     4621 gagaacctgt ccaacgccct gaccgaagcg ctcgcgccgc tgcctcaggc cacgctcgaa
     4681 gacgtcgccg aggcgatgac ccagctggac gaattccgcg acgcgcttgc cgagcacgaa
     4741 gccttgcgcg acgccgtggc ccagttcgac cggcgctacc gcgcctacgc ccgcgtccag
     4801 gctcgccggc aggcgcgcgt gctgcgccag gcgcagaccg agttcgacaa cgcgagtcgc
     4861 gcgctgggag aggcgcgcgg cgagctggcc gccgcggaac agacggtcgg ggagcttaag
     4921 gcgcgctgcg ccgagctcga ccggcagctc gcactcgatc gcgccgcgct cgacgagctg
     4981 cgccaggatc ccgtaatgcg cgatgctcgg cgcctccacg agactgtgca cctcgccgag
     5041 gagcgcagtc gcgacgccga ggaagcggga aagcggcgag acgaggcgga gggacgccat
     5101 agcgaagaac gggcggcggc cgccagccgg cgcgaggagt cgcagcagtc gctggcgaga
     5161 ctgacgcgag acgcggggag cgcggagcgc ctcgcgtcgg aggtggggct cgccagtgac
     5221 cacgccctga tcgccgctgt ccttattcct ttcgcggatc tgggtcagcg cacgcccacc
     5281 caactggaag gtctgacctc ggcgtctcgc gagatggttg agcgccggcg cgcccaagtt
     5341 gcgcagatcc ggcaccgttt gcgcgaaacg gacgacgctc tcagggcccg ggatcgcgcc
     5401 gccgaggcac gcgccgaccg cgccgaaacg tttgaggccg cgcaagcgcg cgctcgcgac
     5461 gccgatctcc ggctcaaagc cgccgccgag gagtacctgc ggcagtgggg cggctttctc
     5521 gatcgcctta cgacgcttgc gctgcccgat gcgcagaccg cgatggagac cttgtctgca
     5581 tgggtggagt ctctcgccgg cgaaaacccc atggtggctt tgctcgcgcg ggcgcagcat
     5641 gaggccgcac gcggcttcgc cgagcgcgag gccgcgctca agcaccgaca agccgagttg
     5701 cgccgtgagg cggacgaact cgttgcggaa cgcgcgcgcc tcgcgagggg cgaggaccgc
     5761 gcgccccccg cgccgcatac ccgcgcggcc gacgttcgcc gggatcgggc cggctcgccg
     5821 ctgtggcagc tgctggaatt ccgcgaatcc gtgcaggaat cggcgcgcgc cggcctggaa
     5881 gccgcgcttg aggcggcggg gctgctggac gcgtgggtga cccccgaagg cgcgatcctc
     5941 gacccggata cccaagacgt gctcctgatt ccgcgcgcgg cgcgcgcctc gtcgttgtgc
     6001 gattggctcc ttccggtcga aggcggcacc gttccggcat cccggatcgc gggcattctt
     6061 gccggcatct cctgcacgct acaggatccg ggcgatgccg aagcctgggt ttcgcccgac
     6121 gggcgctttc gcctgggacc ggcgcaaggc gcgtggcgca agcccgtagc gcaatacatc
     6181 ggctacgccg cgcgtgtggc ggctcgcgtg cggcggctcg atgaaattgc cgcagagctt
     6241 tccgcgctcg cgtccgagct agagcgggtc gccgcggact tgcgggcgct ggacgggagc
     6301 cggcgcgcgc tgaaccaaga atgcgctcat gcgccgggcg acggcgcgtt gcgcaacgcg
     6361 cacgcgaagt tttccgccga cgaatcgcaa aggcgcgagg cgcatgcgcg gctggcggaa
     6421 gccgaggcgc gatgcggcga cgccgagcgg gcctggagcc gggcgcgcga gaccctgcaa
     6481 cgcgatgccg aggacctgcg cctgcccgcg gatgcggatg ggttgtcgga agtcgaaggc
     6541 gggctcttgg actacgatcg cgccgccctg accgtcgtgc aggcggcgcg cgagcaccgt
     6601 cgcgcggtga tcgagacgct cgaacaggag cgccgcgagc gcgagacgca ggcgcaggcc
     6661 gacaccgctc gcgaggagtg cgcgcggcgt gtacgcgctg ccgaggaggc cgttgcgcgc
     6721 cgcgatctgc tgcgcgagac ggttggcgcg gccgtcgccg agttggaaca tcggctcgcg
     6781 cagaaagaaa aggcggtgca gtccggcgag cagatcctga agggagagaa caagtgtctg
     6841 gtcgaagcgc gcgagcggcg cgcacggagc gggcaaaagg aggaggacgc ccaggcgttc
     6901 ctgagggagc gccagcagcg ccgccaggaa gcggtcgagt atctgcgcgc cttcgcacag
     6961 accgggctcc tgtcggtcgc gctgcccgag ctggaggcac ccgatccggc cgccgcctgg
     7021 acgatcgacc ccgcgctgag cgccgcccgc cggatcgaac atctgctcgc ggaggttccg
     7081 gccgaggata ccgattggac gcgcattcag aacgatgttt cgcgcgactt caccgcgctt
     7141 acccaggcga tgtccgcgca gggacaacag gctcaggcgg agaccagcga ctacggtctg
     7201 attgtgcgca tcgtgtacca gaatcgcccg gaacggccgg atgtgctgga gcgtcggctg
     7261 gggacggaga tcagggagcg ccgcgagatc ctcactgccc gcgagcgcga agtgctggaa
     7321 aaccacctgc aggcggaggt cgcggacggg ctgcagcgcc tgctgcaaga cgccgagcgc
     7381 cgggtgcaga cgatcaacgc ggagctgagc cggcgcccca cctccaccgg cgtgcgtttc
     7441 aagctggaat ggcagccgct gccggaaggc gaggacggcg ctccggcagg cctcgccgag
     7501 gcgcgcaagc gtctgctcaa caccgccgcg gacgcgtggt cggccgagga ccggcgactg
     7561 gttggcgagt tcctgcagaa ccgcatcgca agcgagcgct cccgtgacga cggcggcgcc
     7621 ttgctcgatc acctcacgcg cgcgctcgac taccggcgct ggcatcgctt ccgcgtcaag
     7681 cggtttcagg acggcgcctg gcggccgctg tccggcccgg cttcgagtgg cgagcgcgcg
     7741 ctcggtctta ccgtgccttt gttcgccgcg gcgagcagcc actacgcgag cgccgaatgc
     7801 ccctatgcgc cgcggctcgt gttgctcgac gaggccttcg ccgggatcga cgacgaggcg
     7861 cgcgcccact gcatggcgct gatccgcgag ttcgatctcg atttcgttat gaccagcgaa
     7921 cgcgagtggg gctgctatgc cgagttgccg ggactcgcca tctgccacct cgtgcgccga
     7981 gaaggcgtgg acgcggtctt cgtctcgcgc tggagctggg acgggcgcgc gcgccgcgaa
     8041 gagcccgatc cctcgcgccg ctttccggaa accgccatcg catcatcgtg agcaccgcgc
     8101 gcgagaggct ggaacgtctg ctgggcggac cggctcttgc cgggttgcgc gagcgcctgc
     8161 gccgacgcta cgagcttggc cgtaacaacg acgcgttcac gctcgccgga atttcgccgc
     8221 aggagcgccg cgctctcgag ggtttgctcg ggcgccgcgg ccggcgctcg ggttccatgc
     8281 ggctcaccgt ctcggcactc gacgaagcgc tcgcccgcgc cgggctagcg agctcgctgc
     8341 gcgatgcgct cgaacagatc gacgggccga tccgggacct tacggccgag cgcgcggacc
     8401 gcttggcgca atggaacgct gtctttgcgg tttgtcggaa cgagcccctg gccgcgttcg
     8461 tcgcgcaaag cgcggcctgt ggactcgtca agcgtctggc agcctcggac cccgcgcaag
     8521 gccggatgct gctcgacgcg gcctcgcaag tgctcgcgcg cctgccggcg gcgggcgtgc
     8581 cgctttcgca gcttgcggcg gaagtcctgt gcgatgccca tgcgctggat gaagggcgtc
     8641 ccgtcgcgac gctcgtgttg gccaccctga aaagcggtga tgaagaggag cggtcccgtg
     8701 aggtctgggc gaaccggggg gtgctggtta atgaactcgc cgcacctgcg ctcgtgctga
     8761 atctgcctgc gagcgccgat actgccggcg ggcggctcgt ggcgcaagcg cgcgaactgg
     8821 gtgaaccgtt gcacctgtct ctccgtacgc tcctgcgcgc gccgccgcag tgggaagtaa
     8881 ccggccgtac cgtgttcgtc tgcgagaacc cggcggtggt cgcgatggcg gcggatcgat
     8941 tgtccacttt ttgtgcgccg ttggtctgca ccgatggaat gcccgccgcc gcccagcgtg
     9001 cgcttctcgt tcaacttgcc gccgcggggg taacgctgcg ttatcacgga gacttcgatt
     9061 ggcccgggct caccattggc aatttcgtga tgcgaacttt tggagcgaag ccgtggcgct
     9121 tcggttccga agactacgtc gcaacgtacg gccgccgact gcaaggtgcg gccgttccag
     9181 ccgcctggga tgcggctttg gggccgagga tggcgcagca cggctatgca ctggacgagg
     9241 aagccgtagt cgaggcactg ctgcatgacc ttacctttcg acaagcatga agcgggttgg
     9301 atagggttgg agagggtcaa atctttgcgg gctgttgccc aaatcgttga tcctttctcg
     9361 gaatcatgat agagcggaaa aattggggtc agagaacaat taagtgggaa gcctagttag
     9421 cgccgcgtcc tcgtgatgcc gtcctggggc gttcctgaaa attaagtttc gtcaggagtt
     9481 ggggagtcgg ggtgtatgac cttggcccgc tcccgaggtc aggctttgtc gactcgtatg
     9541 aaaacggtct catggaaagt cgctttcgcc gggctgctgg gtgggttcat cggcaacggc
     9601 gtgctgggcg cgctgttttc gagtccgccg atccgtcaca tcctctacga cccgaccatc
     9661 cagagcctgc tgtttatcga ggtcacgtct cagcgcaaca ttcccatctc ggtggcgggc
     9721 ttggtcgtgt tgagcgtcat ccacgcatgg ttgttcgccg tcttgtttcc cgctcttccg
     9781 gggaggacgt gggtccgcaa gggagcgttc tggggactcg tgatctggct gatgtactgg
     9841 gtgtttcagg agtggttcat ttaccatacg ctgcttggcg agccgtggct gttgaacctc
     9901 ctcgaattgg ccgtcctctt gctcggttcg gtcgtggaag gcgtggtgat cgcgttcgcg
     9961 ttggcgcggg acgcacggca cgtcgaaccc gcttcggtcg gtcggtgagg cgctcgtccg
    10021 gtcgcgggga gttacgatcg tttctcgcga gggctgggat gaatggaagg ctaagagggt
    10081 agcacgcgct gaacagtgtt cgcatcgaag atgacttaga cgccaaacct tttttcgacc
    10141 gcgcgcgggg tattgatcaa ttgataaaag ggggaatcga atggacctca cagggatgag
    10201 agggggcaca gaccgggaaa ggtgctctga gcccgatgtg ctccgtcggc gccgtcgatg
    10261 aagaacattt tgattaccgg cgccaccgga cagatcgggt ctgaattgac gccggccttg
    10321 cgcaaacggt acggtggcgg acacgtcgtg gccgcgggcc acacacgtca cccggatcaa
    10381 gacatcgccg aatcaggtcc atactgctcg ttggacgtca gggacctggc cgccctccaa
    10441 accaccgtcg ccacacaccg tatcgacacg attgttcacc tggcctccgt gctctccgcg
    10501 gcggctgaag agaagccgca actcgcctgg gatatcaacg tccacggctt gatgaacgtg
    10561 ctggaaaccg cccgtctgca ccactgtgcg gtgttcttcc ccagctcgat cgccgcgttc
    10621 ggtccgctca cgccggccga gaacacgccg caggatacga ttcaacgccc cacgacgatg
    10681 tacgggatta cgaaagtcac cggcgaattg ttgtgcgact attatcacgc ccggtatggt
    10741 gtggacacgc gcggcctcag gttcccgggg ctcatctcct cgaagacgtt gcccggcggc
    10801 gggaccaccg attatgcggt gcacatcttc catgccgcgc tgaaggcaag gcagtacgag
    10861 tgctttctca aaccggacac gcggctcgac atgatgtaca tgcccgatgc catcaacgcg
    10921 gcggttcaat tgatggaggc ggacggtcgc cggctgacgc atcggaatgc ctacaacgtc
    10981 accgccatga gcgtggcgcc acgcgatatt gcggaagcaa tcaaacagtt cattcccgac
    11041 ttcacgatca gctacgccat cgatccggcc cgccaggcca tcgccgactc gtggccccga
    11101 cacctggacg atcgcgccgc tcgcgaggag tggggctggc ggccggcgtt cgatttgacc
    11161 gccacggtca aggatatgct ggacacactg tcgtccaggc tcaagtccgc gtgagttgcc
    11221 atgaacctcg gttcagccgc gcatgatgaa atcccccctc accccccttt gacaaagggg
    11281 ggatgggggg attttccgag caggggaggg gcatgtcgct gacacgattc gacgcgctgg
    11341 tcacgggcaa gctcgaagaa ctggaggccc gcggcacgct gaagggccga gaacacgtca
    11401 tcaccggcgt gagcgccggt gaaggcggtg gcagcccacg gtatttcctg gcgggctgcg
    11461 gggatcgcgc gtttctccga atgaacgcca actcgtattt gggcctggcc ttccatcggc
    11521 gcgtcatcga agcccacgcc ctggcgacgg aacgctttgg cgcggggccg ggcgcggtgc
    11581 ggtttatcag cggcacgtat ctccctcacg tcgagttgga gcgcaggctg gcggcgtttc
    11641 atggcagaga cgccgcgatg atcatgagcg cggcgtacgc gaccgtcatc ggcgtgctcc
    11701 cccagttcgt cggcgaagac acggtggtca tcagcgatgc gctcaatcac aactgcatca
    11761 tcaacgcgat ccgcctctcc cgcccggcca agaaagcggt ctatgcgcat cgggacatgg
    11821 ccgccctgaa gcacctcctc gaaacccaca aggggcaggt caggcgcgcc gtggtggtga
    11881 ccgacggggt attcagcatg cgcggggatc atgccccgct ggacgacatc gcggcggtgt
    11941 gcgcgcggca cgaaccgggg tacgccgagg gaatcatcac cgtggtggac gattcccacg
    12001 gcgtcggcgc gttcggcgcc accgggcgag gcacggaaga atacaccggg acgcgcgccg
    12061 atatcctgat tggcaccttg ggcaaggcct tcggggtcaa tggcgggtac gtggtctcaa
    12121 gcgcgccggt gatcaactat ctgagagaaa ccgctccgtg ttatgtctac tccaacccga
    12181 tcacgccggc ggaggccgcc gcggcggtca ccgcactgga catcctggac agcccggaag
    12241 gccggaccct gttggagcac atgcgacggc ttgccgcgcg gctcagggcg ggactcactg
    12301 atctggggct ggaaaccgtc aggagcgagc acccgatcgt ccccatcctg atccgagcca
    12361 ctcgccgaac gtccgcgttg gctcggtatc tcttcgagaa gaatattctc gtcaccgggc
    12421 tgaactatcc ggtggtgccg aaaggcgaag aagaaatccg gctccaggtg tcggcgaacc
    12481 ataccgaaca agacatcgag tccgtgttgg acgcggtgaa acgcttccag gaagaacgcg
    12541 gggcggaatc ggtgaatgaa tacggctcgc gctgacggga ggacccgcgg ataacgccgc
    12601 gagcggatcg atcacctccc gccgtttcct tgacctccac ggcctcctgg gtctatgctg
    12661 gccgcaccat tcagcccgcc ggggggacct gcctgtgcgg agttcttttc cacgtgggcg
    12721 cccaggcggc ggataagcgc cgcaagcgta tcacaatgga tgccaggaat agatgtttca
    12781 atgcaggaca tgaccccgcg gccgtgcgag tggcgtgagc gagaacatca aaaattcaag
    12841 gccagacccc gcatgcgttc aggagatcac tcccgttttc cttctgcaaa cttgctggcg
    12901 tgcagc
//
LOCUS       DBNGWZ010000661         4465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2510956, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000661 DBNGWZ010000000
VERSION     DBNGWZ010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2510956"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="AC16CX_20620"
     CDS             <1..1249
                     /locus_tag="AC16CX_20620"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR3014945.1"
                     /translation="FTNHLDYRQYYLTVDETEAAGRVRRLVAQPNASFLPGNSNPVAR
                     HVAYGGAPRRLHVIVLLEESLGAEFVGAYGDRRGLTPNLDRIARESLIFTRTYATGTR
                     TVRGMEAVTASFPPVPAESIVKRRYNEGIFNWSTVMRENGYSPTFIYGGYGTFDNMNH
                     FFGTNGYRAIDRTNMDEPKFSNIWGVSDEDLFRNAFRVYDEQIARGERIFSVVMTTSN
                     HKPFTFPEGIPGVAPRGGGRLAGVRYADYAIGRFFEQLLTRPWSGDTLVVIVADHGAR
                     VYGREDIPLKSYEIPFIVHSPRHVAAGRVDVLTSQMDVAPTVLGLLKISYDSAFFGKD
                     TLAVASGERLAALNHNRDIALLRSGMLAELRFQKHYAEFRYDPAHNTQQPVAADPEAI
                     RDTASLFQLAYDVFSSGHYRVLQ"
     gene            1246..3687
                     /locus_tag="AC16CX_20625"
     CDS             1246..3687
                     /locus_tag="AC16CX_20625"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HIR3014946.1"
                     /translation="MSATASFATNPSASLPGFALTHAVLPLCAAALALIVFETTRVDY
                     TISGWFFDAATGRFPLRYSPGLEIIMHQWAKYVIVLIAGGAIATWLFSFSNESLRERR
                     HLLLFLSLALVAAPATVSLVKTGGAMHCPYDLVEYGGSATRLGLFERAGPSTAPGRCF
                     LSGHASAGFCLMAFYFVGLAMGNRRLAYGGLLGGVGVGLIFGFARIAQGAHFLSQVVW
                     AGFVSWYVILVIYLALYWRTLGPQHPAADVIESRGESMVRNAPDHPVSASRPARPVIV
                     AALVALLAVLWFGQLESRGLIEPDEGRYAEIAREMVATGDWITPRLNGLLYFEKPPLQ
                     YWATAAAYTVFGPHNWTARLWSALTGLAGILLLMYAGSRLFSARAGCYAALVLASTLL
                     YFGTGQTNSLDMGLTFFLGLVVCSMALGLRAEASSAGTRLWIHVAWLSAALAVLSKGL
                     VGIVLPAGALVAYCIAWPDRSIWKKLHFPTGAPLFLLVAAPWFLAVSNANPDFARFFF
                     IHEHFERFLTTVHDRYEPWWFFIAVLCLGALPWTLPAIAGTWRAIGRRGAGPFRPDAF
                     LAMWIIVVLVFFSLSSSKLIGYIVPVVPAMALLCGRYISESAPRTLAFQFLITAVTAL
                     IGVVLAATLTHVAHRGYPHELVEMKAMWLTAAAVVWLAGAAYAYLAARRARILHAVTA
                     LAIGSLIAYQLVLAGYDVLASHKSSETLASRIKPYVGADTPVYTVGTYPQSLPFYLGR
                     TVTLVNYTGELEFGLEREPGKGIRDLSGFIARWNAEPGAVAVMHVHTRQLLAAAGVPM
                     SVIAENASIVAVRRP"
     gene            3684..4067
                     /locus_tag="AC16CX_20630"
     CDS             3684..4067
                     /locus_tag="AC16CX_20630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HIR3014947.1"
                     /translation="MNLNSFALIITGVLLNAAAQLMLKAGADRVGPIALDGPSLLGAA
                     RALAISLPIAGGILCYAVSVVVWIVALSRVEVSVAYPMLSIGYVVNALGAWILFGEAL
                     TVTRLAGILVIMLGIWILAGSARVS"
     gene            4057..>4465
                     /locus_tag="AC16CX_20635"
     CDS             4057..>4465
                     /locus_tag="AC16CX_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3014948.1"
                     /translation="MSPDFSRNGALPAAGEAEAFLPFARPTIDEETVAAVAEVLRSGW
                     LASGPKEAAFEAGLSRHCGNRPVRTFTSATAALEVALRVAGVGAGDEVITTAMSFAAS
                     ANVILRVGARPVFVDIGLGSRNIDPLEVEAAITP"
BASE COUNT          675 a         1502 c         1435 g          853 t
ORIGIN      
        1 cttcacgaac cacctcgact acaggcagta ttacctcacg gtggacgaaa cggaagcggc
       61 cgggcgagtg cggcgtcttg tcgcccagcc caatgcctcg tttctgcctg gaaactccaa
      121 tccggtcgcg cgtcacgtcg cctatggcgg agcgcccagg cggctgcatg tcatcgtgct
      181 gctcgaggaa tcgctcggag cggagttcgt cggggcctac ggagaccggc gcgggcttac
      241 accgaacctg gaccggattg cccgcgaaag cctgatattt acccgcacct atgccaccgg
      301 tacgcgcacg gtgcgcggga tggaagccgt gaccgcctcc ttcccgcccg tacccgccga
      361 atccatcgtc aagcgccgct acaacgaggg gatcttcaac tggtccacgg tcatgcgcga
      421 aaatggctac agcccgacct ttatttacgg cggctacggc actttcgaca acatgaatca
      481 tttcttcggc acgaacggct accgggcgat tgaccgcacc aatatggatg aaccgaagtt
      541 cagcaacatc tggggcgtga gcgacgagga cctgttccgc aacgcgtttc gggtgtacga
      601 cgaacagatc gcgcgcggcg agcgcatctt ttccgtagtg atgacgacct ccaaccacaa
      661 gccgttcacc ttcccggagg gcattccggg cgtggcgccg cgcggcggcg ggcggctggc
      721 cggcgtgcgc tacgccgact acgcgatcgg gcgcttcttt gagcagctgc tcacccgccc
      781 ctggtccggc gacacgctgg tggtcatcgt cgccgatcac ggcgcacgcg tctacgggcg
      841 cgaggatatc ccgctcaagt cctacgagat accattcatt gtccacagcc cccggcacgt
      901 ggcggcgggc cgcgtggacg ttctgaccag ccagatggac gtggcgccga ctgtcctcgg
      961 actgctgaag atttcctacg acagcgcctt ctttggcaag gacacgctcg ccgttgcgtc
     1021 cggcgaacgg ctggccgcgc tcaaccacaa ccgggacatc gcgctgctca gatccggcat
     1081 gctggcggag ctccgcttcc agaagcacta cgccgaattc cgctacgacc cggcgcacaa
     1141 cacccagcag ccggttgccg ctgatccgga agccatacgc gacacggcga gcctgttcca
     1201 gctcgcctac gatgttttca gcagcggcca ttaccgggtg ctgcaatgag cgccactgcg
     1261 tcattcgcta ctaacccctc ggcttcacta cccggattcg cattgaccca cgcggtgctg
     1321 ccgctgtgcg ccgccgcact ggcgctcatc gtcttcgaga cgacgcgcgt cgattacacg
     1381 atcagcggct ggttcttcga cgcggccacg ggcaggttcc ccctgcgcta cagccccggg
     1441 ctggaaatca tcatgcatca gtgggccaag tacgtcatcg tgctgatcgc cggcggcgcg
     1501 atcgcgacct ggctgttctc gttcagcaac gagagcctgc gggaacggcg gcacctgctt
     1561 ctgttcctga gccttgcact ggtggccgcc ccggccaccg tcagcctcgt caagaccggg
     1621 ggcgccatgc actgccccta cgatctcgtc gaatacggcg gctcggcgac gcgcctcggc
     1681 ctgttcgagc gcgccggccc gtccactgca ccgggccgat gtttcctgag cgggcacgcg
     1741 tcggcgggat tctgtctcat ggccttctat ttcgtgggcc tggcaatggg gaatcgccgt
     1801 cttgcgtacg gcggcctgct cggcggggtg ggcgtgggcc tgatattcgg attcgcgcgt
     1861 atcgctcaag gcgcccattt cctgtcacaa gtcgtttggg ccggattcgt ttcatggtac
     1921 gttatcctcg tgatctacct ggcactgtac tggcggacgc tcggcccgca gcatccggca
     1981 gccgacgtga tcgaatcgcg cggtgaatcc atggtccgga acgcgcccga tcatccggtc
     2041 tccgccagcc gccccgcccg acccgtcatc gttgcggccc tggtggcgct cctggcggta
     2101 ctctggttcg gacagctgga atcgcgcggg ctgatcgagc cggacgaggg ccgctatgcg
     2161 gagatagcgc gcgagatggt cgccaccggc gactggatca cgccgcgcct gaacggcctg
     2221 ctctacttcg agaagccgcc actgcagtac tgggccacgg ccgccgctta cacggtattc
     2281 gggccgcata actggaccgc gcggctgtgg agcgcgctga caggtctggc cggaattctg
     2341 cttttgatgt acgccggcag ccggctcttt tcagcccgcg ccggctgcta cgcggctctc
     2401 gtcctggcaa gcaccctgct ctacttcggg accggtcaga ccaattcgct tgatatgggg
     2461 ctgacgttct tcctcgggct cgtcgtctgc tcgatggcgc tggggctcag ggcggaggcg
     2521 tcgagcgccg gcacgcgatt gtggatccac gtcgcatggc tgtccgcggc gcttgccgtc
     2581 ctgagcaagg gactggtcgg catcgtcctt cccgcaggcg cgctggtggc ctactgcatc
     2641 gcgtggccgg atcgctcgat ctggaaaaag ctccacttcc cgaccggagc gccgctgttc
     2701 cttctggtgg ccgcgccgtg gtttctcgcg gtgagcaacg cgaaccctga tttcgcccgc
     2761 ttcttcttca tccatgaaca tttcgaacgg tttctgacga ccgtgcacga ccgctacgag
     2821 ccgtggtggt tcttcatcgc tgtactgtgt ctcggcgcat tgccatggac cctgcccgcg
     2881 atcgcgggca cgtggcgggc gatcggacgg cgcggggccg gtcctttccg gccggacgcg
     2941 tttctcgcca tgtggatcat cgtggtgctc gtgtttttca gcctgtcgag ctccaagctg
     3001 atcggataca tcgtcccggt cgtgcctgcg atggccctcc tgtgcggccg ctacatcagc
     3061 gagagcgccc cgcggaccct cgcgttccag ttcctgatca ccgccgtgac cgctctgatc
     3121 ggcgtggtcc tcgccgcgac cctgacgcac gtcgcgcacc gcggctatcc gcacgagctg
     3181 gttgagatga aggcgatgtg gctgacggcg gcggccgtgg tctggctcgc aggcgctgca
     3241 tatgcctatc ttgcggcacg gcgcgcccgg atcttgcacg ccgtcacggc ccttgcgatc
     3301 ggcagcttga ttgcatacca gctggtgctg gccggatacg atgtgcttgc ttcacacaag
     3361 tccagcgaga cgctggccag ccggatcaag ccctacgtgg gtgcggacac gccggtatac
     3421 actgtcggca cgtatcccca gagcctgccc ttctatctcg ggcgcaccgt aacgctcgtc
     3481 aactacacgg gcgagcttga attcgggctc gagcgcgaac ccggcaaggg tatacgcgac
     3541 ctgagcggct tcatcgcgcg ctggaacgcc gagccgggcg ccgtcgccgt gatgcatgtg
     3601 catacccgtc agttgctggc ggcggcgggc gtgcccatgt cggtcatcgc cgaaaacgcc
     3661 agcattgtgg ccgtgaggag accatgaacc tgaactcatt tgcgctgatc atcaccggtg
     3721 tgctgctgaa cgcggccgcc cagctgatgc tgaaagcggg cgcggatcgc gtcggcccga
     3781 ttgcgctgga cgggcccagc ctgctcgggg ccgcccgggc actcgcgatc tcgctgccga
     3841 tcgctggagg gattctctgc tacgcggtaa gcgtcgtggt ctggatcgtg gcgttgtcgc
     3901 gagtcgaggt cagcgtggcc tatcccatgc tttccatcgg atacgtcgtc aacgcgctcg
     3961 gcgcctggat cctgttcggc gaggcgctca ccgtcacccg gttggcgggg atcctggtca
     4021 tcatgctggg gatctggatc ctggcgggga gcgcgcgtgt ctcctgattt ttcccgcaac
     4081 ggcgcgctgc ccgccgccgg cgaagcagag gcgttcctgc cctttgcgcg cccgacgata
     4141 gacgaggaaa cggtggcggc ggtcgccgag gtgcttcgct ccggatggct cgcaagcggg
     4201 cccaaggagg ccgctttcga ggccgggctg tcccggcact gcgggaatcg cccggttcgc
     4261 actttcacca gcgcgaccgc cgccctggag gtcgcgctgc gggtggcggg cgtgggcgcg
     4321 ggagacgaag tgatcacgac agcgatgagc ttcgccgcgt ccgccaacgt catcctgcgg
     4381 gtgggcgcgc gccccgtgtt cgtcgatatc ggcctcggct cccgcaatat cgacccgctc
     4441 gaggtggagg ccgccatcac gccgc
//
LOCUS       DBNGWZ010000662         4982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_370727, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000662 DBNGWZ010000000
VERSION     DBNGWZ010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_370727"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..864
                     /locus_tag="AC16CX_20640"
     CDS             598..864
                     /locus_tag="AC16CX_20640"
                     /inference="COORDINATES: protein motif:HMM:NF024886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HIR3014949.1"
                     /translation="MEQSHESKGGFPAQPQGPHLLDRVREAIRRRHYSRCTEETYIHR
                     IKRFIYFSGKRHTMPRYDPGSSFSWRPGGSIILLLLSPSRRFKY"
     gene            complement(884..1711)
                     /locus_tag="AC16CX_20645"
     CDS             complement(884..1711)
                     /locus_tag="AC16CX_20645"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3014950.1"
                     /translation="MKNRLALHTWTLDTTPLAGVLRAAKAAGWNAVELRRVDFTRCFE
                     AGMSNAQVLDLVRASGIKVACLGTEYGLIFAKGEERERLLKVLDETCANAQALGCDLV
                     MIATGQNSSTNVRDAAANFKAGGEVAKRHGVRFALEFNSQHDLINCLDRGREVVEFAN
                     HPNCGLLLDAYHLERSGAGGRGFESYPAEKIFAFQFSDVPAGPPAAVRRPTDRLPPGK
                     GIVRWREVFQLLQEKGYEGYLSYEAPNPEYWSRPAVEVAREAVAATRRLLAEVEYKT"
     gene            complement(1708..2784)
                     /locus_tag="AC16CX_20650"
     CDS             complement(1708..2784)
                     /locus_tag="AC16CX_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014951.1"
                     /translation="MRLVVPFAQGGRTGSRRGGWCWGGDGALPPGLWHCILLRIAIAL
                     VAATTFSAGTSFAQPYPSKPVRLIVPFAPGGNVDINARALSPGLAQLLGQSFVVENRA
                     GAGGTIATDLVAKAPPDGYTLLVASSSVMTNGPAVYPKLPYDIVRDFAPVGRIAVVPL
                     AIVVHPSLPARNARELIALAKAQGGKLLMANAGVGTTNHLIAELFMIRTGARMTLIPY
                     KGSGPALIDLVAGHVYGHVDQISSALPYIRADRIRAIAVTTRTRAAQLPEVPTLAESG
                     VPGFDASTVTGVLAPAKTPRDIVQKLNAALGQVLAQAAVKERFATLGAEVHPSTSDEL
                     GAWIREDLAVWVKVVKQAGIKLEL"
     gene            3064..4377
                     /locus_tag="AC16CX_20655"
     CDS             3064..4377
                     /locus_tag="AC16CX_20655"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3014952.1"
                     /translation="MTESHRIVIVGGGAGGLELATRLGDTLGRKGRARVTLVDRSRTH
                     LWKPLLHEFAAGSMDLDHHALSYLAQARWHHFRFQRGSMDGLDRGRRVILVAPTFDED
                     GRELIPRREIPYDTLVIAVGSNTNDFGTPGAREHAISLDMPEQAELFHRRMINACIRA
                     NAQVAPLRPEQLKVAIIGAGATGVELAAELHKTTRELVAYGLDRINPEKDIKLTVIEA
                     APRILPAVPERLSKAAVELLRGLDVQVLTGERVTGVAADGVTTAAGRKIPAELTVWAA
                     GIKAPDFLKDISGLETNRLNQLVVRETLDTTRDPDVFAMGDCASCPWPGHDRTVPPTA
                     QAAHQQASHLARMMKRRLAGKPLEPWRYRDFGSLVSLGEYSTVGSLMGALLGGTIFIE
                     GLFARFMYVSLYRMHVYALHGLAKTLFDTLAKLITRRTEPRVKLH"
     gene            4464..4838
                     /gene="trxA"
                     /locus_tag="AC16CX_20660"
     CDS             4464..4838
                     /gene="trxA"
                     /locus_tag="AC16CX_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3014953.1"
                     /translation="MATVELSKENFEEVITKNDMVIVDFWAPWCGPCRGFAPVFEAAS
                     EKHGGIVFGKVNADDEQELAAAFNIRSIPFLMFFREKVILFAQAGALPAEGLESVIRQ
                     VRALDMAKVHKEVAEQEAQQQQ"
BASE COUNT          939 a         1555 c         1567 g          921 t
ORIGIN      
        1 ccgctgaagc tcggccgcct caacgagcgc cacatgcgca tcgaagatca atatgggaga
       61 ctaaagcgca aaactgtgta acccatgtcc cccgacactt tcgttaccta tctccccggc
      121 tgctcaaacc aacatgggac cctattgcca tgcgtacgcc agaatactga agggcaaata
      181 atccggcgaa tatcacgcca actatggtga agtgtttcgc atttgaccac gctgcggtcc
      241 ggggcacgac tatcggaatg gtctcgccaa taatcgtggc acatccaaac gcgttcaggc
      301 cgacaaaaat ggcggtctca gcagctgacc agcgcggtac ggagccacca gagaaattgt
      361 ttagggttgc ccataggccg aagaagaaag cgattccgcc caggatgagc acaaatagac
      421 gcacgcccct cgatcgataa tcatgcgggg aattggccat aagcgctcta actttgtaat
      481 ggttagacga aattctactt aacacggtaa ttccgtcccc tgcctacgtc aacgcgccct
      541 cgaatcaagg cgttatgacc ctgtaatggg tatgaggtcc gtgttaggta gcagaaaatg
      601 gaacaatcac atgaatccaa aggagggttc cccgcgcaac cgcaagggcc acacctgcta
      661 gatcgcgtgc gcgaagccat acgccgcagg cactacagcc ggtgcacgga agagacctac
      721 atccaccgga tcaaacgctt catctacttt tccggcaaac gccataccat gccgcgatat
      781 gatcctggtt ccagtttctc ttggcgtccc ggcggttcaa ttattctttt gcttctctcc
      841 ccatctcggc ggttcaagta ttgattttct ttgcggttca gttttacgtc ttgtattcga
      901 cttccgcgag caggcgccgc gtcgccgcca cggcttcgcg cgcgacttcc acggcgggcc
      961 gggaccagta ctcgggattg ggcgcctcgt agctgagata accctcgtag cccttctcct
     1021 gcaggagctg gaagacctcg cgccagcgca cgataccctt gcccggcggc aggcgatcgg
     1081 tcggcctccg gaccgcggcc ggagggcctg ccgggacgtc gctgaactgg aacgcaaaga
     1141 ttttctccgc tggatatgat tcgaagccgc ggccgccggc gccactgcgc tccagatggt
     1201 aggcatccag cagcaggccg caattcggat ggtttgcgaa ttccacgact tcgcggcctc
     1261 ggtcgaggca gttgatgagg tcgtgctgcg agttgaattc gagtgcgaac cggacgccgt
     1321 gcctcttcgc gacttcgccg cccgccttga agttggccgc ggcgtcgcgc acgttggtgg
     1381 aagaattttg ccccgtggcg atcatcacga gatcgcagcc cagggcctga gcgttggcgc
     1441 aggtctcgtc gagcactttc agcaatcgtt cccgctcctc gcccttcgcg aagatcagcc
     1501 cgtactcggt accaaggcag gcgaccttga ttccgcttgc gcgcaccaga tcgagaacct
     1561 gggcgttgct catgccggcc tcgaagcagc gcgtgaaatc cacgcgccgc agctcgaccg
     1621 cgttccagcc ggccgctttc gccgcgcgca gcaccccggc gagcggcgtc gtgtcgagcg
     1681 tccacgtgtg gagcgccagc cgattcttca tagttcaagt ttgatgccgg cctgcttcac
     1741 caccttgacc caaacggcga gatcctcccg gatccacgcg ccgagctcgt cgctcgtgct
     1801 cggatgaact tcagccccaa gtgtggcgaa gcgctccttc acggcggcct gcgccaagac
     1861 ctggccgagg gcggcattga gtttttgcac gatgtcgcgc ggcgtcttgg ccggggcgag
     1921 tacgccggtg accgtactgg catcgaatcc cggcacgcct gattcggcca gcgtcggcac
     1981 ttcgggcagt tgcgccgcgc gcgtccgggt ggtgaccgcg atcgcccgga tacggtcggc
     2041 gcgtatatac ggcagagcgg aggaaatctg gtccacgtgc ccgtagacgt gtccggccac
     2101 gagatcgatc aaggcggggc cgctgccttt gtagggaatg agcgtcatgc gcgcgccggt
     2161 ccggatcatg aacagctcgg cgatcagatg gttggtcgtt cccacgccgg cattcgccat
     2221 gaggagcttt ccgccctggg ccttggcaag cgcgatcagt tccctcgcgt tccgcgcggg
     2281 cagggaaggg tgcaccacga tggcgagcgg cacgacggcg atgcggccca ccggcgcgaa
     2341 gtcgcgcacg atgtcgtagg gcagcttggg atacaccgcg gggccgttgg tcatgacgct
     2401 gctcgaggcg acgagcaggg tgtacccgtc gggcggcgct ttcgcgacca ggtccgtcgc
     2461 gatggtccca ccggcgcccg cgcggttttc caccacgaag gactgcccca gcaattgggc
     2521 gagaccgggc gagagtgcgc gtgcgttgat gtccacgttg ccgcccggcg cgaacggcac
     2581 gatcagccgc accggcttcg atggataggg ttgcgcgaac gatgttcccg ctgagaatgt
     2641 cgtcgcggcg accagcgcga tggcaatccg cagcagaatg caatgccata aaccgggcgg
     2701 cagcgccccg tctccgcccc aacaccaacc ccctcgccga gatccggtgc gccccccttg
     2761 cgcaaacggc acgacgaggc gcactgcgcg cgttggataa ttctgtgcga cggcgtttga
     2821 aactgcgcat gccattgcga taacgcaaac cgctgcggcg cgggtatttg tcatatcaga
     2881 ttcctcccat cgttcagact gaacagtatc gcgcgttgag cggcgctgat atcgtccgct
     2941 ccaacgcgtg tggtcaaacc ggcgcgctgc gtccggtttg acgcttattc tacggtcatt
     3001 tccgtcaacg cggctttagt gccccgccta taatggatgt ggaatgcaat ttgaccgttt
     3061 gcaatgacgg aatcgcaccg cattgtcatc gtcggaggtg gcgcgggcgg gctcgagctc
     3121 gccactcggc tcggcgacac gctcggccgc aagggcaggg cacgggtcac gctcgttgat
     3181 cgctcgcgca ctcacctgtg gaagccgctg ttgcacgagt ttgcggcggg cagcatggat
     3241 ctcgaccatc acgcgctgtc ctatctggca caggcgcggt ggcaccattt ccggttccag
     3301 cgcggcagca tggacgggct cgaccgcggg cgccgcgtga tcctggtggc gccgacgttc
     3361 gacgaggacg gccgcgaatt gattccgcgc cgcgagattc cctacgacac gctggtgatc
     3421 gccgtcggca gcaatacgaa cgactttggc acgccggggg cgcgcgagca tgcgatcagc
     3481 ctcgatatgc cggagcaggc ggagctgttt catcggcgga tgataaatgc ctgtatccgc
     3541 gccaacgctc aggtggcgcc gctgcgcccg gagcagctga aagtggccat catcggcgcg
     3601 ggcgcgaccg gcgtcgagct tgcggcggag ctgcacaaga ccacgcgcga gctggtcgcc
     3661 tacgggctcg accggatcaa tcccgagaag gacatcaagc tgacggtcat tgaggccgcg
     3721 ccgcgcatcc tcccggcagt gccggagcgc ctgtcgaagg cggcggtgga gctgttacgc
     3781 gggctcgacg tgcaggtgct caccggcgag cgcgtgacgg gagttgccgc cgacggggtc
     3841 acgacggcgg cgggcaggaa gattcccgcc gagctgaccg tgtgggccgc cggcatcaag
     3901 gcacccgatt tcctcaagga catcagtggc ctcgaaacca accgcctgaa ccagctggtg
     3961 gtccgggaaa cgctcgacac cacgcgtgac ccggatgtct tcgccatggg cgactgcgcg
     4021 agctgcccgt ggccggggca cgatcgaacc gttccgccga ccgcccaggc cgcacatcag
     4081 caggcctcgc atcttgcgcg gatgatgaaa cgccgtcttg caggcaagcc gctcgagccg
     4141 tggcgctatc gcgatttcgg ctcgctggtt tcgctcggag agtactccac cgtcggcagc
     4201 ctgatgggcg cgctgctcgg agggaccatc ttcatcgaag gactgttcgc gagattcatg
     4261 tacgtctcgc tctaccgcat gcacgtctac gcgctgcacg gtttagccaa gaccctgttc
     4321 gacacgctcg cgaagctgat tacgcggcgt accgagccgc gagtcaagct gcactgacgc
     4381 gcagcttgac taagggatga gggcggaggg atgagggatg aggggcatgt taagatgcgc
     4441 gacctttccg atcggagtac gcgatggcca ccgtcgaact gagcaaggaa aactttgaag
     4501 aagtcattac caagaacgac atggtgatcg tggacttctg ggcgccgtgg tgcggtccct
     4561 gccgcggttt cgcgccggtg ttcgaggcgg cctccgagaa gcacggcggc atcgtgttcg
     4621 gcaaggtgaa cgcggacgac gagcaggaac tggcggcggc gttcaacatc cgctccattc
     4681 cgttcctgat gtttttccgc gagaaggtaa tcctcttcgc ccaggccggc gcgctgccgg
     4741 cggaaggcct ggaaagcgtc atccgccagg tacgcgcgct cgacatggcg aaggtccaca
     4801 aggaagttgc cgaacaggaa gcgcagcaac aacagtgagg cgtgataagt gatgagtgat
     4861 aagtgatgag tgataagtga tgagtgttta ggaccggcgc tgccggtgat tcttattacc
     4921 cgtcacccat cacccatcac ccatcaccga gcaacagctg ctcgagtgct ttgggctgct
     4981 gc
//
LOCUS       DBNGWZ010000663         7992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2383869, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000663 DBNGWZ010000000
VERSION     DBNGWZ010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2383869"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="AC16CX_20665"
     CDS             <1..736
                     /locus_tag="AC16CX_20665"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR3014954.1"
                     /translation="AQIDPSSVRTIMMGSSPSSPQLLRELRMHFLNAEPLVVYGVTEG
                     GPVPLGPHPEGKPRPIGSLGVPYPGTEAKLIGGSSPDEGELVLKNPGNLLGYHNLPEE
                     TAKRLRDGWYYTGDVCHRDADGFYFFVGRTDDMFVSGGENVYPGEVEALLQRHPAVHQ
                     AIVMPFDHELKGQVPYAFVVRRAGAVVTEDEIKRFALANGPAYQHPRRVFFLGQFPLA
                     GTNKIDRDRLREWVADGVLKPVTDAA"
     gene            804..1223
                     /locus_tag="AC16CX_20670"
     CDS             804..1223
                     /locus_tag="AC16CX_20670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3014955.1"
                     /translation="MSEQQISAEKLQELITRGPFNRWLNFTVVKADAEGIEVRAGWRE
                     EWVVNPERRYTHGGILAAIVDTVADYAIAAKLGRPVPTIDVRVDFHKAAMPGDLTAKA
                     RVVRMGSQYSTAEAYLYDKDGALVASGRGTYFTAPPK"
     gene            1351..1989
                     /locus_tag="AC16CX_20675"
     CDS             1351..1989
                     /locus_tag="AC16CX_20675"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3014956.1"
                     /translation="MKKTQKSSGSRKKRLIVAMTGATGSIYGVRILEMLRAIGGWETH
                     LIVSDAGALNAWYDLKLARKDLAKFADVVHNVRDVAASISSGSFVTHGMVIAPCSMKT
                     LAAVAHGFSDNLISRAADVILKERRRLVLIVREAPLNLAHLRNMVQVTEMGAIVYPPV
                     PAFYSHAKTVDELVNHTVGRVLDLFGVEHSRIKRWQGMKGAAPANEEAGKLR"
     gene            1986..3620
                     /locus_tag="AC16CX_20680"
     CDS             1986..3620
                     /locus_tag="AC16CX_20680"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR3014957.1"
                     /translation="MKMEKLDVKHESFRQFLDRLRAEKELTDIRQPVDIRHIATLVDQ
                     AETALLFHEVIGYDLPVVSGIIRSRNRAILSMGCSDYPEIEHRLQHGIDHPIAPKHVK
                     TTRNKEVIEIDDAVDLYRLPIPMSSIYDGGPMITAGVVIAKNPEYGFNSGMYRFMVKE
                     KNLTGIDIVTPNNMRLFAQRAFEAGRPCPISISIGTHPFEIMGSGFRAPLGVDEMAIA
                     GGIRGAPVELAMCETIDMPCLADAEIVLEAEILPTGWTHPEGRFGEFTRLMGGLHWNP
                     LVEVKAITRRKDAIYYALHMPWENTWLAAPTRYTAIRRALKNAGVQVKDINVTLGGCG
                     FWHAVVSIRKQAGEGKNALLAALTAQDIKHVVVVDDDVDVFDPTDVEWAIATRVQGDK
                     DVIIIPGARAKPLDPSLPVTPPGVVPTGAKVGIDATIGEGIPRERFERIAYAYADRAK
                     VADYIKGKADAAPKAAGHDERIAADLAGKILKLIEAEPKYYQEIAEHFGEYEFQAVAR
                     AMGRLHAEEKLWQDARGRMCVRGSAFAAKPPAKPEQ"
     gene            3746..4741
                     /locus_tag="AC16CX_20685"
     CDS             3746..4741
                     /locus_tag="AC16CX_20685"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3014958.1"
                     /translation="MPSRKFRVHIENLRARPELYHVTAERWSAACGRHRVLARYIEAS
                     IGWDGEVLDEALQETDFLIGIRTRLERLAERAPRLKWVHVTLAGVDRFLPLDWLPPGV
                     VLTNNHGAHGVKAEQYMRMAYTLLHTRMPEILANQRSHRWQQLFSPSIRGKTALIVGL
                     GDLGQGAARAAEQLGLKVVGVSRTGKKIRHADAVYKTAALDRLLPQADYVVLAVPLTP
                     ETRHLLDRRRLSRMKPRAAVINIARAPVLDNDALVEKLRRGELAGAVLDVVEPEPLPP
                     ESPLWDAPNLILTPHISCDDGEHYVDISLDLWFANFARFLRRKPLRNRVDPRRGY"
     gene            4905..5777
                     /locus_tag="AC16CX_20690"
     CDS             4905..5777
                     /locus_tag="AC16CX_20690"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3014959.1"
                     /translation="MLDHVAHFVAHIDAASSALETLGFTLTPFSAHSHRSEPGGPLAP
                     AGAGNRCVVLERGYLEIVTPTGDTQHAGQLRAAIKRYTGAHLIAFGTAAPEADHARIH
                     RAGFAPLPPVALQRQIGTESGEGTVRFTVVRVPPGTMPEGRIQFCQHHTPELVWQERW
                     LEHPNCASALDGVVLCVADPGEAAQRYARYTGLLAQVSGGACRLATARGYLLFIDAAT
                     LERRLGVNPPSLPWIAGCVLESGDIAATGDVLRNAGVHVHVLGNRRLMVKLPASVGGF
                     IIFEPKGSGILNFD"
     gene            5792..6031
                     /locus_tag="AC16CX_20695"
     CDS             5792..6031
                     /locus_tag="AC16CX_20695"
                     /inference="COORDINATES: protein motif:HMM:NF044585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6812 domain-containing protein"
                     /protein_id="HIR3014960.1"
                     /translation="MDKDENRTKVTILTSSYRIRGYIDVLPGSRVTDFIVQSKDFIAV
                     TDAEVFEPELGGRQVLTASFIDVHRDHIQILAPAP"
     gene            6062..7624
                     /locus_tag="AC16CX_20700"
     CDS             6062..7624
                     /locus_tag="AC16CX_20700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3014961.1"
                     /translation="MQFDEGFDVVVAGFGLAGGIAAITAADAGAKVLLIEKSEVPGGL
                     SICSYGAVRSAREPEAAFAYLKATNDGRTPDDVLQVLAEGMCGIEAYVRELARINNAV
                     ISTSVAEAASAPDDPHRRRVGANYPFPGNEAFYHTTVVDVPGFEAAKHYPWANGAPGG
                     PKLFKIIHDHLRKLGVEVRLATPALRLIADPGTREVRGLRVAANGAERAIRARRGVIL
                     ACGGFEGNAEMKAQFLEGKPIFNAAARTNSGDGIRMAQDFGAALWHMWHIHGAYGFRH
                     PDPAYPYAIRLKRFPDWFPGEEKRARVKMAWILLDQDGRRFMSEYQPYTQDTGVRPSQ
                     YYDPVRQRLPRNPAFMICDEEGRKLYPLGKATSNDKGIRYYWSDDNLKEVESGILKRA
                     DTLEGLATKLGLEPETMVASVSRWNEQCARGRDEDFGRPGGTMTPICTPPFYGAPVWA
                     TVSNTQGGPVHDAQSRIIDVYGKPISRLYAAGELGSSFGHLYLSGGNIAECFVTGRIA
                     GRNAATLAPWNT"
BASE COUNT         1517 a         2609 c         2564 g         1302 t
ORIGIN      
        1 cgcgcaaatt gatccgtcgt cggtgcgcac catcatgatg ggctcgtcgc cgtcctcgcc
       61 ccagctctta agggaattgc ggatgcattt cctgaatgcc gagccgctgg tggtttacgg
      121 cgtaaccgaa ggcgggccgg tcccgctcgg tccccacccg gagggcaaac cacgtccgat
      181 cggatcgctc ggcgtgccct accccggcac cgaggcaaag ctcatcggtg gttcgagccc
      241 ggatgaaggc gaactcgtgc tcaagaatcc cggaaacctg ctcggctatc acaatcttcc
      301 ggaggagacc gcgaagcggc tacgcgacgg ctggtactac accggcgacg tctgccatcg
      361 cgacgccgac ggcttctatt ttttcgtcgg gcgcaccgac gacatgttcg tctccggcgg
      421 cgagaacgtc tacccgggag aagtcgaggc gctgctccaa cgccatcccg ccgtgcacca
      481 ggccatcgtc atgccgttcg atcacgagct gaaaggccaa gtgccctacg cctttgtcgt
      541 gcggcgggcc ggcgccgtcg tgaccgagga cgagatcaag cggttcgcgc tggcaaacgg
      601 gcccgcttac cagcacccgc ggcgcgtctt tttcctcggc cagtttccgc tcgccggcac
      661 caacaagatc gaccgggacc ggctgcggga atgggtggcc gatggcgtgc ttaaacccgt
      721 gactgatgcg gcgtgactga tgaagcgtga ctgatactgc ggaagtcttg attctggggc
      781 ccctgagaaa ttgggatcat gaaatgagcg aacagcagat aagcgcggag aagctgcaag
      841 aattaattac tcgtggaccg ttcaaccgct ggctcaactt caccgtggtg aaggcggatg
      901 cggaggggat cgaggtgagg gccgggtggc gcgaggaatg ggtagtcaat cccgaacgcc
      961 gctacactca cggaggcatc ctcgccgcga tcgtggacac ggtggccgac tacgctatcg
     1021 cggcgaagct cgggcgcccg gtccccacga tagacgtgcg ggtggacttc cacaaagcag
     1081 cgatgcccgg cgacctcacc gcgaaggcgc gcgtggtacg tatgggcagc cagtactcga
     1141 ccgcggaagc gtacctgtac gacaaggacg gcgcgctggt ggcgagcggg cgcggcacgt
     1201 atttcaccgc tccgccgaag tgaagaggcg tgataggtga caagtgataa gtctcccgcg
     1261 ctccgcgcgg atgtcgagtc ctatcaccca tcacatatca cttatcactt atcacgcatt
     1321 taggactcag tgtaggatag agccttgatc atgaagaaga cacaaaaatc ctccggatcg
     1381 cgcaaaaagc gccttatcgt ggcgatgact ggcgcgaccg gctcgatcta cggcgtgcgc
     1441 atcctcgaaa tgctgcgcgc aatcggcggc tgggaaacgc acctcatcgt gtccgatgcc
     1501 ggcgcgctca acgcttggta cgacctcaag ctcgcgcgca aggacctcgc gaagttcgcc
     1561 gacgtggtgc acaacgtgcg cgacgtcgcc gcatcgattt caagcggctc attcgtcacc
     1621 cacggcatgg tgatcgctcc atgctcgatg aaaacgctcg ccgcggtggc gcacggcttc
     1681 tcggacaatc tgatctcgcg cgcggcggac gtgatactga aggagcggcg gcggctcgtg
     1741 ctcatcgtgc gggaggcgcc gctcaacctc gcgcatctgc gcaacatggt gcaggtaact
     1801 gagatgggcg ccatcgtcta tccgccggtg ccggccttct acagccatgc gaagaccgtg
     1861 gacgagctgg tgaaccatac ggtcggccgc gtgctcgacc tctttggcgt cgagcacagc
     1921 cgcatcaagc gctggcaggg gatgaaaggc gcggcgcccg ccaacgaaga agccgggaag
     1981 ctccgatgaa gatggaaaag ctcgacgtga agcacgagag cttccggcag ttcctggacc
     2041 ggctgcgcgc cgaaaaggaa ctgacggaca tccggcagcc ggtggacatc cgtcatatcg
     2101 ccacgctggt ggaccaggcg gaaaccgccc tgctctttca cgaggtcatc ggctacgacc
     2161 tgccggtcgt ctccggcatc atccgctcgc gcaaccgcgc catcctctcg atgggctgca
     2221 gcgattaccc ggagatcgag caccgcctgc agcacggcat agaccatccg atcgcgccga
     2281 agcacgtaaa gacgacgcgc aacaaggagg tgatagagat cgacgacgcc gtggacctct
     2341 accgcctgcc gatcccgatg tcctcgatct acgacggcgg cccgatgatc accgccggcg
     2401 tggtgatcgc caagaacccg gaatacggct tcaattcggg catgtaccgc ttcatggtca
     2461 aggagaagaa cctgaccggc atcgacatcg tgacgccgaa caacatgcga ctgttcgcac
     2521 agcgcgcctt cgaggctggg cgcccctgcc cgatctcgat ttccatcggc actcatccgt
     2581 tcgagatcat gggctccggt ttccgcgcgc cgctgggcgt ggacgagatg gcgatcgccg
     2641 gcggcatccg cggcgcgccg gtagagctcg cgatgtgcga gaccatagac atgccgtgtc
     2701 tcgctgacgc cgagatcgtg ctcgaggccg aaatcctgcc gaccgggtgg acccatcccg
     2761 aggggcgctt cggcgagttc acgcgcctga tgggagggct gcactggaac ccgctggtgg
     2821 aggtgaaggc gatcacccgg cgcaaggacg cgatctacta cgcgctgcac atgccgtggg
     2881 aaaacacctg gctcgccgcg cccacgcgct acaccgcgat tcgcagggcg ctcaagaacg
     2941 ccggggtgca ggtgaaggat atcaacgtga cactcggcgg ctgcggcttc tggcacgccg
     3001 tcgtctcgat ccgcaagcag gcgggcgaag gcaagaatgc gctgctcgct gcgctgaccg
     3061 cgcaggatat caagcacgtg gtggtggtgg acgacgatgt cgacgtcttc gatccgaccg
     3121 acgtcgagtg ggcgatcgcc acacgcgtgc agggcgacaa ggatgtcatc atcattcccg
     3181 gcgcgcgcgc gaagccgctc gaccccagcc tccccgtcac gccgccgggg gtggtgccga
     3241 ccggcgccaa ggtcggcatc gacgcgacga tcggcgaagg catcccacgc gagcgcttcg
     3301 agcgcatcgc ctacgcttac gccgaccgcg cgaaggttgc cgactacatc aagggcaagg
     3361 ccgacgccgc tccgaaagcg gcaggccacg acgagaggat cgcggcggat ctcgcgggca
     3421 agatcctcaa gctgatcgag gccgagccga agtactacca ggagatcgcc gagcacttcg
     3481 gcgaatacga attccaggca gtggcgcgcg ccatgggcag gctgcacgcc gaggaaaagc
     3541 tgtggcagga cgcgcgcggg cgcatgtgcg tgcgcggctc ggccttcgcg gcgaagccgc
     3601 ccgcgaagcc cgagcagtaa gcggtaagcc gtaagcgggg agcggaaaac gccgaagcaa
     3661 caagcgcctg ccgctcaaac ccgcatcagc cgggttccac tgcataccgc tcacggctaa
     3721 ccgctcaccg ttcaccgcca tcaacatgcc ctcccgcaaa ttccgcgtgc acatcgaaaa
     3781 cctgcgcgca cggcccgagc tttaccacgt cacggctgag cgctggtccg cggcctgcgg
     3841 ccggcaccgc gtgctggcgc gatatatcga ggccagcatc ggctgggacg gagaagtcct
     3901 ggacgaggct ctccaagaga ccgactttct gatcggcatc cgcacccgcc tcgagcggct
     3961 tgccgaacgt gcgccacggc tcaagtgggt gcacgtcacg ctggccgggg tggaccggtt
     4021 cctcccgctc gactggctcc cacccggggt cgtgctcacc aataatcatg gcgcgcacgg
     4081 cgtgaaggcc gagcagtaca tgcgcatggc ctacaccctg ctccacacgc gcatgcccga
     4141 aatcctcgcc aaccagcgct cgcaccgctg gcaacagctg ttctcgccga gcatccgggg
     4201 caagaccgcg ctcatcgtcg ggttgggcga tctcggccag ggtgccgcgc gcgcggcgga
     4261 gcagcttggt ctcaaggtcg tcggcgtcag ccgcaccgga aaaaaaatcc gccatgccga
     4321 cgcggtgtac aagaccgccg cgctcgatcg gctgttgccg caagcggatt acgtcgtgct
     4381 ggccgtgccg ctcacgccgg aaacccggca tctcctcgat cgccggcgcc tcagccggat
     4441 gaagccgaga gctgcggtga tcaacatcgc gcgcgcgccg gtattggata atgacgcgct
     4501 cgtggaaaag ctccggcgcg gcgagctcgc cggcgcggtg ctcgatgtcg tagagcccga
     4561 gcccttgccg cccgaatcgc ccctgtggga cgcgcccaac ctgatcctca ccccgcacat
     4621 ctcgtgcgac gacggtgagc actacgtgga tatctcgctc gacctatggt tcgccaactt
     4681 cgcccgcttc ctgcgaagga agccgctcag gaaccgcgtc gatccccgcc gcgggtatta
     4741 gccgccgatg aacgccgatg cacaccgaac atttcaagac gaaaccgcca agaacggcac
     4801 aaatttatcg aaccaccaag acgccaagag aaacaacaat tttctactct atgaactccg
     4861 tgtcctctgt ggctgataac caggttccgg ctgcgggaga gctggtgctg gaccacgtcg
     4921 cgcatttcgt ggcgcacatc gacgccgcga gcagcgcgct ggagacgctc ggtttcaccc
     4981 tgacgccctt ctccgcgcac tcgcaccgtt ccgagccggg cgggccgctc gcgcccgccg
     5041 gcgcgggaaa ccgctgcgtg gtgctggagc gcgggtatct ggaaatcgtc acgccgaccg
     5101 gcgacacaca gcacgccggc cagctccgcg ccgcgatcaa gcggtatacg ggagcgcacc
     5161 tcattgcgtt cggcaccgcg gcacccgaag ccgatcacgc caggatccat agggccggtt
     5221 tcgcaccgct gccgcccgtg gcgctccagc gccagatcgg caccgagtcc ggagaaggca
     5281 cggtgcgctt cacggtggtg cgcgtgcctc ccggaacgat gcccgagggc cgcatccagt
     5341 tctgccagca ccatacgccg gaactggtct ggcaggagcg ctggctggaa caccccaact
     5401 gcgcgagcgc actcgacggc gtggtgctgt gcgtcgccga tcccggcgag gcagcgcagc
     5461 gctacgcccg ctataccggg ctgctcgcac aggtaagcgg cggcgcctgt cgcctggcca
     5521 ccgcgcgcgg ctacctgctg ttcatcgacg cggcgacgct ggagcgccgg ctcggcgtaa
     5581 atccgccttc gctgccgtgg atcgccggct gcgtgctgga aagcggggac atcgcggcga
     5641 ccggcgatgt tctgcgcaat gcgggcgttc acgtgcacgt gctcggcaac cggcggctga
     5701 tggtcaagct ccccgcgagc gtcggcgggt ttatcatctt cgagccgaaa ggttcgggca
     5761 tcctcaactt tgactagaag gagtgcgatt tatggataaa gatgaaaaca ggaccaaggt
     5821 gaccatcctc accagcagtt accgcatcag gggttatatc gacgttttgc ccggctcccg
     5881 ggtgacggac ttcatcgtcc agtccaagga cttcatcgcc gtgaccgacg cggaggtctt
     5941 cgaaccggaa ctgggaggcc gccaggtgct caccgcgtcg ttcatcgacg ttcatcgcga
     6001 ccacattcag atcctggcgc cggcgccctg atcttcgacc acgctcatcg gcggtttcga
     6061 aatgcagttt gacgagggct tcgacgtcgt cgtcgccggc ttcggcctgg cgggcggcat
     6121 tgccgcgatc accgccgcgg atgccggcgc caaggtcctg ctcatcgaga aatcggaggt
     6181 gccgggcggg ctctcgatct gctcctacgg cgcggtgaga agcgcgcgcg agcccgaggc
     6241 ggcgtttgcc tacctcaagg cgaccaacga cgggcgcacg ccggacgacg tgctgcaggt
     6301 gcttgccgag ggcatgtgcg ggatcgaggc ttacgtgcgc gagctcgcgc gcatcaacaa
     6361 cgccgtcatc agcacgtcgg tcgcggaagc cgccagcgcg ccggacgatc cccaccggcg
     6421 ccgcgtcggg gccaactatc ccttccccgg caacgaggcg ttctaccata ccaccgtcgt
     6481 ggacgtgccg gggttcgagg cggcgaagca ttatccgtgg gccaacggcg cgccgggcgg
     6541 gcccaagctc ttcaagatca tccatgacca tttgcgcaag ctcggcgtcg aggtccggct
     6601 cgccaccccg gcgctgcgtc tcatcgccga tcccgggaca cgcgaagtgc gcgggttgag
     6661 ggttgcggcg aacggcgccg agcgcgcgat ccgggcgcgg cgaggggtga tcctcgcgtg
     6721 cggcggcttc gagggcaacg cggagatgaa ggcgcagttc ctcgaaggca aacccatttt
     6781 caacgcggcg gcgcgtacca atagcggcga cggcatccgc atggcgcagg atttcggcgc
     6841 agcgctgtgg cacatgtggc acattcacgg cgcctacggc ttccgccatc ccgatcccgc
     6901 ctatccctac gcgatccggc tcaagcgctt ccccgactgg ttccccggcg aggagaagcg
     6961 ggcgcgggtg aagatggcgt ggatactgct cgaccaggat ggccggcgct tcatgtcgga
     7021 gtaccagccg tacacccagg acacaggagt caggccgtcg caatactacg acccggtgcg
     7081 gcagcgctta ccgcgcaatc ccgcgttcat gatctgcgac gaggagggac ggaagctgta
     7141 cccgctcggc aaggcgacct ccaacgacaa gggcatccgt tactactgga gcgacgacaa
     7201 cctgaaggag gtcgagtcgg ggatattgaa gcgcgccgac acgctcgaag ggctcgcgac
     7261 aaagcttgga ctcgagccgg aaaccatggt ggcaagcgtc tctcgctgga acgagcagtg
     7321 cgcgcgtggg cgcgacgagg acttcgggcg ccccggcgga accatgacgc ccatatgcac
     7381 gccgcctttc tacggcgcgc cggtgtgggc gacggtttcc aacacccagg gcggcccggt
     7441 gcacgacgcg cagagtcgta tcatcgacgt ctacggcaag ccgatctccc gcctctatgc
     7501 cgccggcgag ctcggcagct ccttcggcca cctctatctt tcggggggca acatcgccga
     7561 gtgcttcgtc accggccgca tcgccggaag gaacgccgcg acactcgcgc cgtggaatac
     7621 gtgagatcgg gagagcggga ggtcgggagg tcgggaggtc gggaggtcga ctttcaccct
     7681 gattttttat acaacttttt cgcctgacgc attgaaattc ttgagggatg acaacgtcac
     7741 gcgcacaatg ccgatttgca aaccggcttt taggccaact acttcacgtt cggtggcaca
     7801 ttgggaacgc catcagtatc aacctcccga cggcgcatga ttccggtgat tcgcggtaag
     7861 ccagtgccga tggccgctta cgacctattc tgttgaaaaa ctctttttcc accgacgatg
     7921 aaaaaattct agccgttata ggaagcgagg cgcgcttcag gctaggggga tacatgaaag
     7981 agttgatgtc gc
//
LOCUS       DBNGWZ010000664         2553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2890080, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000664 DBNGWZ010000000
VERSION     DBNGWZ010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2890080"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="AC16CX_20705"
     CDS             complement(<1..1213)
                     /locus_tag="AC16CX_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3014962.1"
                     /translation="MKFVLGLWYRVRVEGDAAPLRDGRVLIVANHDSMLDGALLSLFL
                     PAEVTVAIAGDDLRRPLIRLLMRGIPHVILDPRRPLALKQLIRLVKRGRPVVVFPQGP
                     ATPTGSLTKIYDSAAVIAARCNARIVPVRITGTLYTRFSAVGGNFPKRLFPPVRIRIL
                     PGQQPPAPSPLPGKKRRRLLADSLLKIMQHMMFASRPPTTLFEALLDAARLHGRSTRI
                     LEDTQREHSYGDLIKASLALGRLGSKLADEGEIVGVMVPNLATTVALLLGLSAMRRVP
                     AMINYTSGPDALRSSCIAAGIKTLITSRRFVEVAKLGGSVAALSTVRIVYLEDLRARF
                     GLADKLWLAAWALWRPRAVIRARDPEETAVVLFTSGSEARPKGVALSHRALLANIAQL
                     GAVIDFGPNDKF"
     gene            1519..2445
                     /locus_tag="AC16CX_20710"
     CDS             1519..2445
                     /locus_tag="AC16CX_20710"
                     /inference="COORDINATES: protein motif:HMM:NF006452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR3014963.1"
                     /translation="MEMHVPSAVLEVRHVPASQPLATGVALSFSPQLRVHYDKETRAI
                     WSRWAPEPRPCFNTALLADIRAYYDFLGSSKARIDCFGEEHAIEYVVLASATPGVFNL
                     GGDLDLFKQLIDTRDRQGLLRYGRACIDVLYRNYIGHELPVTTVSVVQGECLGGGFEA
                     ALSSDVIIAEKSVRFGFPEILFNLFPGMGAYSFLERRIGQRGAEELIASGKIYSAEEM
                     AAFGVIDIVVEDGSGESEVAALVRRRARSRNGLSALAAVRRRVHRIEFSELLDIVQTW
                     VEAALRLSPRDLKLMQRLVSRQNVLNEPHQIH"
BASE COUNT          473 a          816 c          812 g          452 t
ORIGIN      
        1 ggaacttgtc gttggggccg aaatcaatga ccgcgcccag ttgcgcgatg ttggcgagca
       61 aggcgcgatg gctcagcgca acccccttgg gtcgtgcctc cgatccggac gtgaagagca
      121 ccaccgcggt ttcctcgggg tcgcgcgcgc ggatcacggc gcgcggccgc cacagcgccc
      181 atgccgcgag ccacagcttg tcagccagac cgaatcgggc gcgtaagtcc tccaagtaaa
      241 cgatgcgcac ggtgctgagc gcggcgacgg agccgcccag cttcgcgact tcgacaaagc
      301 gccgggacgt gatcagcgtt ttgatgccgg cggcgatgca ggagctgcgc aacgcatcag
      361 ggcccgaagt gtagttgatc atggccggaa cgcgccgcat cgcggagagt cccagcagca
      421 gcgcgacggt cgtcgcgagg ttgggcacca tcacgccgac gatttccccc tcgtccgcga
      481 gcttgctgcc cagccgtccc agcgcgaggc tggccttgat cagatcgccg taggaatgct
      541 cgcgctgcgt gtcctcgagg atgcgggtgg agcggccgtg cagccgggcg gcgtcgagca
      601 gggcctcgaa cagcgtggtc ggcggcctcg aggcaaacat catgtgctgc atgattttca
      661 gcaacgagtc cgccagcagg cgccgcctct tcttgcccgg caacggcgag ggcgccggcg
      721 gttgctggcc cggcagtatg cggatcctta ccggcgggaa caggcgcttg ggaaagtttc
      781 cgccaaccgc ggaaaaacgc gtgtacagag tgcccgtgat gcgcaccggc acgatgcgcg
      841 cgttgcagcg cgcggcgatg acggcggccg agtcgtaaat cttggtgagg ctgccggtcg
      901 gagtcgccgg cccctgcggg aacaccacga ccggcctgcc gcgcttcacg agccggatca
      961 gctgtttcag cgcgagcgga cgccgcggat ccaggatgac gtgcggaata ccgcgcatca
     1021 gcagccggat cagcggacga cgcagatcgt cgccggcgat ggcgaccgtg acttccgcgg
     1081 gcaagaacag gctgagcaac gcgccatcca gcatcgaatc gtgattggcg acgatcagca
     1141 ctctcccgtc gcgcaggggg gccgcatccc cctcgacccg tacgcggtac cagagcccga
     1201 ggacaaattt caaaaaccgg atgatcacaa agacttactt ggtatagtta atccagatta
     1261 ttagctgggg cccggtgagc gtcaaacttt ccacttggta gcaagccctg ctacgtgttg
     1321 cgcgatacgc cacacggcgc ttcattatca ctcactccgg cccacggcgg tcaagttctt
     1381 accctctgtt tctaaaccga aaagttaaaa tctatcaaaa tgttagattt gctttctcat
     1441 gactgattgg catttaatca aagtgtgacg tagatcacac acatgatctt cactcgtttt
     1501 tatcagggga gaatcattat ggaaatgcac gtcccatccg cggtactgga agtccgtcat
     1561 gttcccgcca gccagccctt ggccaccggc gtcgcgctca gcttctcgcc ccagctgcgc
     1621 gtgcattacg acaaggaaac gcgggccatc tggtcacgct gggcgccgga gccgcgcccc
     1681 tgcttcaaca ccgccctgct cgccgacatc cgcgcctatt atgactttct gggctcgagc
     1741 aaggcgcgga tcgattgctt cggcgaggag cacgccatcg agtatgtcgt gctcgcctcg
     1801 gccacgcccg gcgtgttcaa cctgggcgga gatctcgacc tgttcaagca gttgatcgat
     1861 acccgcgacc gccagggcct gttgcgctac gggcgcgcct gcatcgacgt cctctaccgc
     1921 aactacatcg ggcacgagct gcccgtcacc accgtttcgg tggtgcaggg cgagtgcctg
     1981 ggcggtggat tcgaggccgc gctctcgagc gacgtcatca tcgccgagaa gagcgtcagg
     2041 ttcggttttc ccgagatcct gttcaacctg ttcccgggca tgggcgccta ttccttcctg
     2101 gagcgccgca tcggccagcg cggcgccgaa gagctgatcg ccagcggcaa gatctacagc
     2161 gcggaggaaa tggccgcgtt cggagtgatc gacatcgtgg tggaagacgg cagcggcgag
     2221 tcagaggtgg ccgccctggt ccggcgccgg gcccgcagcc gcaatggtct ctcggcgctg
     2281 gcggcggtgc gccggcgcgt gcaccggatc gagttctcgg agctgctcga catcgtgcag
     2341 acctgggtgg aagcggcttt gcggctcagt ccgcgcgact tgaagctcat gcagcgcctg
     2401 gtttcccgcc agaacgtgct gaacgagccg caccagatcc actaggagtc tgtcgaatca
     2461 gggtccggca aactcctcgg ccattggcca tcgcatgacg gccctgatag actaatgcgg
     2521 cacacaatgc acaagagcca gcggcctgcg gcc
//
LOCUS       DBNGWZ010000665         3749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3018199, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000665 DBNGWZ010000000
VERSION     DBNGWZ010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3018199"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="AC16CX_20715"
     CDS             <1..179
                     /locus_tag="AC16CX_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR3014964.1"
                     /translation="KLLQAARMLAQQPQAMQLRYLQTLGSIAGDKSNTIVFPVPMDLL
                     SSFLDFARGNKPSA"
     gene            288..767
                     /locus_tag="AC16CX_20720"
     CDS             288..767
                     /locus_tag="AC16CX_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR3014965.1"
                     /translation="MTRYVFVAALALLLTGAAGITQSAGALATKGTGGVAYVSGGIGE
                     TEQQRLAAREQEFNLKLVFTLVEGNYVADVGVALKSATAKMVLEHVAEGPIFLARLPA
                     GTYTAAVSYGGRSQTRSIRVGEKLRTEYFRWPSNPDTDLAVSRWLEPAAGAKSEARQ"
     gene            851..2224
                     /locus_tag="AC16CX_20725"
     CDS             851..2224
                     /locus_tag="AC16CX_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HIR3014966.1"
                     /translation="MILTSVARRVPAQWLASGALLTVSLPALAAQPAVVPEGPALFGI
                     PVDFILFALTLIGVALFHHRTLEVALTGLAAITAYKLAFTGFKTGPGFAGLAAHMQHE
                     WVILTNLLCLLMGFALLSNHFEKSHVPEVLPRFLPDDWKGAFVLLVMIFVLSSFLDNI
                     AAALIGGTIAATVFRHKVHIGYLAAIVAASNAGGAGSVVGDTTTTMMWLEGVDPLDVL
                     HAYVAAGTAIAIFGIPASLQQQRYSPIMKDEPRHARVDWARVGIVAFILVAAIATNVV
                     VNLRFKHLSDAFPFIGAAVWVAILACVPLRKPQWDVLPEAFKGSIFLLSLVTCASLMP
                     VEKLPAASWQTAMGLGFVSAVFDNIPLTALALKQGGYDWGFLAYAVGFGGSMIWFGSS
                     AGVALSNMYPEAKSVGSWLRHGWHVAIAYVLGFFVLLWTLGWEPHEPHKPSSATPVPV
                     SAPAQAC"
     gene            2547..3716
                     /locus_tag="AC16CX_20730"
     CDS             2547..3716
                     /locus_tag="AC16CX_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR3014967.1"
                     /translation="MLKKFLKKKTQSPEKSSESRRKFLTGAAAATTGAAITAFPMIAK
                     AQGPISMRWQSTWPTKDIFHEYALDFAKKVNDMTGGDLRIEVLPAGSVVPAFGLLDAV
                     SKGTLDGGHGVLVYHYGKQNALALWGSSPAFSMDANQLLAWHKYGGGKELLDKVYKSI
                     GANVVSFPYGPMWTQPLGWFKKPITKPDDFKGLKFRTVGISIDLFQGLGAAVNALPGG
                     EIVPAMDRGLLDAAEFNNASSDRLLGFPDVSKVYMLQSYHQNGEQFEITFNKTKFDAL
                     PAKIKAIIENAVEAASQDLMWKSIHRYSQDHIELQVKDKVKMYKTPDSVLLRQLQIFD
                     QVLEKRSKDNPLFKEVAESQRKFAERAVRWYLDVQVSPRMAYNYYFGKKPAAKKA"
BASE COUNT          691 a         1156 c         1201 g          701 t
ORIGIN      
        1 aaaagctcct gcaggcggcg cgcatgctcg cccagcagcc gcaggcgatg cagctgagat
       61 atttgcagac cctcggcagc atcgccggcg acaagagcaa caccatcgtg ttcccggtgc
      121 cgatggatct gctttcgtcg ttcctggact tcgcgcgcgg gaacaagcca agcgcctgat
      181 tgtcccatta gtcagcacaa ttcgtatctg ctgccggtgc gacgttgact ggcggcgcgc
      241 ggtattcgcg ccgtatcggc cgtgaccagc gagcgaagga gcgtgtgatg acacgatacg
      301 tattcgtggc ggcgttggct ttgctcctga cgggggctgc gggaatcaca cagagcgcgg
      361 gcgccctcgc gaccaagggt acgggcggag tcgcctacgt ttccggcggc ataggagaga
      421 ccgagcagca gcgactcgcg gcgcgcgagc aggaattcaa cctcaagctc gtcttcaccc
      481 tggtggaagg gaactacgtc gcggacgtcg gtgttgctct caaaagcgcc acggccaaga
      541 tggtcctcga gcacgtggcc gagggaccaa ttttcctcgc gcggcttccc gccgggacat
      601 atacggcggc cgtaagttac ggcggcaggt cgcaaacgcg ctcgatcagg gtgggcgaaa
      661 agctgcgcac ggaatacttc cgctggccga gcaaccccga caccgatctg gcggtctcgc
      721 gctggctcga gcccgcggcc ggggccaaat ccgaggcgcg gcaataagaa gtttcccgcg
      781 ctcccgcgcg atgtgagcgc gcggctggtg atagaatcgc ccgtcttctc accggctgat
      841 ctctggaata atgattttga cttccgtggc gcgccgcgta ccggcgcagt ggttggcgtc
      901 cggcgcgttg ctcaccgttt cgctgccggc cctggcggcg cagcctgcag tcgtccccga
      961 ggggccggcg ctgttcggaa ttcccgtcga cttcatccta ttcgcgctga cgctgatcgg
     1021 cgtcgcgttg tttcatcacc gcacgctgga agtcgctctg acggggcttg cggccatcac
     1081 ggcttacaag ctcgccttca ccggcttcaa gaccgggccg ggtttcgccg gcctcgccgc
     1141 ccacatgcag cacgaatggg tgattctcac caacctgctg tgcctgctca tgggatttgc
     1201 gctgctgtcg aaccacttcg aaaaaagcca tgtcccggag gtgttgccgc gtttcctgcc
     1261 cgacgactgg aagggcgcat tcgtgttgct cgtcatgatc ttcgtgctct ccagcttcct
     1321 cgacaacatc gccgcggcac tgatcggcgg caccatcgcc gccactgtat tccggcacaa
     1381 agtacacatc ggctatctcg cagcgatcgt ggcggcctcc aacgccggcg gcgcgggcag
     1441 cgtcgtgggc gacaccacca ccaccatgat gtggctcgaa ggcgtggatc cgctcgatgt
     1501 gttgcacgcc tacgtagcgg cgggcacggc gatcgcgatc ttcggcatac ccgcctcgct
     1561 gcagcagcag cgctactccc cgatcatgaa ggatgagccc cgccatgcgc gggtggactg
     1621 ggcccgggtg gggatcgtcg ctttcatcct ggtcgctgcg atcgccacca acgtggtggt
     1681 caacctccgc ttcaagcacc tgtcggacgc ctttcccttc atcggcgcgg cggtgtgggt
     1741 agcgatactc gcgtgcgtgc cactgcgcaa gccgcaatgg gacgtgctgc ccgaggcgtt
     1801 caagggcagc atcttcctgc tgtcgctggt gacctgcgct tcgctcatgc ctgtggaaaa
     1861 actgccggct gcttcgtggc agaccgcaat ggggctggga ttcgtgtccg cggtgttcga
     1921 caacatcccg ctcacggcgc tggcgctgaa acagggcggc tacgactggg gattcctcgc
     1981 ctatgcggtg ggcttcggcg gctccatgat ctggttcggc tcgagtgccg gcgtggcgct
     2041 gtccaacatg tacccggagg ccaagtcggt cggatcgtgg ctgagacatg gctggcacgt
     2101 ggcgattgcc tacgtgctcg gcttcttcgt gctgctgtgg acgctgggat gggagccgca
     2161 cgaaccgcat aagccatcgt ctgcgactcc cgtgccggtc agcgctccag ctcaggcgtg
     2221 ctgaacgggg ccggctggta gcctggaacg caggcgataa tggcggtcgt gaccacgctt
     2281 gccaacccgc ccgcgcccgt gcggttgatt cggcgggggc gggaataatc gccgtcagtc
     2341 ttatgtaagc gtcaagtgct aggatgttcc actgacgggg tccgctgata gcttgctttt
     2401 ggaggtgtcg gagtatacga ttgccgcgga gtagtaaagc gtcccgccta ttaaaaatcc
     2461 gcttatccac cggtgtcttg ggtgctttgc tgcctgtata cccgtatcat gggtgcccct
     2521 gattcataac taggacggag gacgaaatgc tcaagaaatt cctgaagaag aaaacgcagt
     2581 caccggaaaa atcgagcgaa tccagacgca agttcctaac cggcgcggct gcggcgacga
     2641 ccggcgcggc gatcaccgcg ttcccgatga tcgccaaggc gcaaggtccg ataagcatgc
     2701 gctggcagag cacctggcct actaaggaca tcttccacga gtacgccctc gacttcgcga
     2761 agaaggtaaa cgacatgacg ggcggcgatc tcaggatcga ggtgctgccc gcaggctcgg
     2821 tggtgccggc cttcggcctg ctcgacgcgg tatcgaaagg aacgctggac ggcgggcacg
     2881 gcgtgctggt ctatcactac ggcaagcaga acgccctggc gctgtgggga tcgagccccg
     2941 cgttctccat ggacgccaac caactgctcg cttggcacaa gtacggcggc ggcaaggagc
     3001 tgctcgacaa ggtctacaag tccatcggcg ccaacgtggt gtccttcccg tacgggccga
     3061 tgtggacgca gccgctcggc tggttcaaga agccgatcac caagccggac gacttcaagg
     3121 gcctcaagtt ccgcaccgtg ggcatctcga ttgacctgtt ccaaggcctc ggtgcggcgg
     3181 tgaacgcgct gcccggcggc gagatcgtgc cggcgatgga ccgcgggctg ctcgacgccg
     3241 cggagttcaa caacgccagc tccgaccgcc tgctcggctt cccggacgtg tccaaggtgt
     3301 acatgctgca gagctaccac cagaacgggg agcagttcga gatcacgttc aacaagacga
     3361 agttcgacgc gctaccagcg aagatcaagg cgatcatcga gaacgcggtg gaagcggcat
     3421 cgcaggactt gatgtggaaa tctatccacc gctactcgca ggaccatatc gagctgcagg
     3481 tgaaggacaa ggtgaagatg tacaagaccc cggattcggt gctgctgcgg cagctgcaaa
     3541 ttttcgatca ggtcttggaa aagaggtcga aggacaaccc gctgttcaag gaggtcgccg
     3601 aatcgcagcg caaattcgcc gagcgcgcgg tgcggtggta tctcgatgtc caggtcagcc
     3661 cgcgcatggc ctataactat tacttcggga agaagccggc cgcgaagaaa gcctgacgct
     3721 gaacgtgctc gcgggaaaca ccatccggc
//
LOCUS       DBNGWZ010000666         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3268136, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000666 DBNGWZ010000000
VERSION     DBNGWZ010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3268136"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /gene="atpD"
                     /locus_tag="AC16CX_20735"
     CDS             complement(<1..1349)
                     /gene="atpD"
                     /locus_tag="AC16CX_20735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3014968.1"
                     /translation="MAQAQANGTIVQCIGAVIDIEFPRDAMPNIYDALVLEEDANNPL
                     AEKGLTFEVEQQLGDGVVRTIALGSSDGLRRGMKVRSTGEPISVPVGKGTLGRIMDVL
                     GRPIDEAGPVKTETRRSIHQAAPKFDELSPSVELLETGIKVIDLICPFAKGGKIGLFG
                     GAGVGKTVNMLELINNIATKHSGLSVFAGVGERTREGNDFYHEMSDAGVIKLDNLAES
                     KVAMVFGQMNEPPGNRLRVGLTGLTMAESFRDEGRDILFFVDNIYRFTLAGVEVSALL
                     GRMPSAVGYQPTLAEEMGRLQERITSTKTGSITSVQAIYVPADDLTDPSPATTFGHLD
                     ATVVLSRDIASLGIYPAVDPLDSTSRQVDPNTIGEEHYNTTRQVQATLQRYKELRDII
                     AILGMDELSPEDKLAVARARKIQRFLSQPFTVAQNFTGTPGKYVPLKDTIKGFKMIV"
     gene            complement(1414..>1880)
                     /locus_tag="AC16CX_20740"
     CDS             complement(1414..>1880)
                     /locus_tag="AC16CX_20740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR3014969.1"
                     /translation="IVSQVTQLGDRPQMEKLIGTVKVMLDGYIQDRFDRLMIFYTRFI
                     NTMKQEPVMEQLLPLAGEQLGVPESVWDYIYEPEAKVVLDQVLIRYVETLIFQAVAEN
                     MSSEQSARMVAMKAASDNAGNVIDELTLIYNKSRQAAITKEISEIVGGAAAV"
BASE COUNT          309 a          602 c          584 g          385 t
ORIGIN      
        1 ttcacgatca tcttgaagcc cttgatggtg tccttcaacg gcacgtattt gcccggcgtg
       61 ccggtgaagt tctgcgccac ggtgaagggc tgcgacagga acctctggat cttgcgcgcg
      121 cgcgccaccg ccagcttgtc ctccggtgag agctcgtcca tgccgaggat cgcgatgatg
      181 tcgcgcagct ccttgtagcg ctgcaaggtg gcctgcactt ggcgcgtggt gttgtagtgc
      241 tcctcgccga tcgtattcgg atcaacctgg cgcgaggtcg aatcgagcgg gtccaccgcc
      301 gggtagatgc cgagcgaggc gatgtcgcgc gaaagcacca ccgtggcgtc caggtggccg
      361 aaggtggtgg cgggcgaggg atcggtcaag tcgtccgcgg gcacgtagat cgcctgcacc
      421 gaggtgatcg acccggtctt ggtcgaagtg atgcgctcct gcaggcgccc catctcctct
      481 gcgagcgttg gctggtagcc caccgcggaa ggcatccgcc cgagcagcgc cgagacctcc
      541 acgcccgcga gcgtgaagcg gtagatgtta tccacgaaga acaggatgtc gcgtccctcg
      601 tcgcggaagc tctcggccat ggtgaggccg gtcaacccca cgcgcagccg gttgcccggc
      661 ggctcgttca tctggccgaa caccatcgcc accttggact cggccaggtt gtcgagcttg
      721 atcacgcccg cgtccgacat ctcgtggtag aagtcgttgc cctcgcgcgt gcgctcccca
      781 acgcccgcga acaccgacaa tccgctgtgc ttggtggcga tgttgttgat gagctccagc
      841 atgttgacgg tcttgccgac accggcgccg ccgaagaggc cgatcttgcc gcccttcgcg
      901 aacgggcaga tcaagtcgat caccttgatc ccggtctcga gcagctcgac cgacggcgag
      961 agctcgtcga acttgggcgc ggcctggtgg atcgagcgcc gggtctcggt cttgacaggg
     1021 ccggcttcgt cgatcggacg gccgaggacg tccatgatgc gcccgagcgt gcccttgccg
     1081 acgggcaccg agatcggctc gccggtgctc ctcaccttca tgccgcggcg caggccgtcg
     1141 gaggatccga gcgcgatggt acgcaccacg ccgtcgccga gctgctgttc gacctcgaac
     1201 gtgaggccct tctcggcgag cgggttgttg gcgtcttcct cgaggaccag cgcgtcgtag
     1261 atattgggca tggcatcgcg cgggaactcg atgtcgatga ccgcgccgat gcactggacg
     1321 atcgttccat tagcttgtgc ttgtgccata gtcgttccta acaaaactat ttcgttctaa
     1381 accttggcgc cttggcggtt cgcatcgcgg ttttcacacc gccgccgcac cgccgacgat
     1441 ctccgagatc tccttggtaa tcgccgcttg gcgcgacttg ttgtagatca gcgtcagctc
     1501 gtcgattacg ttgccggcat tatcggaagc cgctttcatc gccaccattc gcgcagattg
     1561 ctcggatgac atgttctccg ccaccgcctg gaagatgagg gtctcaacgt atcgtataag
     1621 cacttgatct aacacgactt tcgcctcggg ctcgtaaatg tagtcccaca cgctctccgg
     1681 tacgccgagc tgctcgcccg caagcggcag cagctgctcc atcaccggtt cctgcttcat
     1741 ggtgttgatg aagcgggtgt agaaaatcat cagccggtcg aagcggtcct ggatgtagcc
     1801 gtcgagcatc accttcaccg tgccgatcag cttttccatc tgcggccggt cgccgagctg
     1861 cgtcacctgc gacacgatat
//
LOCUS       DBNGWZ010000667         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_245537, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000667 DBNGWZ010000000
VERSION     DBNGWZ010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_245537"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /gene="arsS"
                     /locus_tag="AC16CX_20745"
     CDS             complement(<1..712)
                     /gene="arsS"
                     /locus_tag="AC16CX_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732795.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HIR3014970.1"
                     /translation="MHATLPRLEVSDFPAIRRRSLETLQVNLGYKCNQTCVHCHVNAG
                     PTRTEMMDWKTVSEVVSFLRASGVNTLDITGGAPELSPYFRSLVLAARNLAVHVIDRC
                     NLTVLEQPGQEDTAQFLAGNRVEVVASLPCYLEENVNAQRGKGVFEASIRALKTLNAL
                     GYGKEGSGLALNLVYNPQGPSLPPAQDKLEADYHRHLGERYGVVFNRLYVLTNMPIQR
                     FGSMLVSKGRFEEYMALLK"
     gene            971..1216
                     /locus_tag="AC16CX_20750"
     CDS             971..1216
                     /locus_tag="AC16CX_20750"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3014971.1"
                     /translation="MSKPSKSQPPQSLEAALAELEKIVSSMEDGQLPLERSLAAYKRG
                     AELLKYCQSALQDAQQQVKVLENGILRDFKGDDNDND"
     gene            1194..2099
                     /locus_tag="AC16CX_20755"
     CDS             1194..2099
                     /locus_tag="AC16CX_20755"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3014972.1"
                     /translation="MTTTTTTDFSAWSHAHLERVEAALQRALPAPEIAPQRLHQAMRY
                     AVLGGGKRVRPLLAFAAGELANADPERVAIAGAAVELIHAYSLVHDDLPCMDDDMLRR
                     GRPTCHVEYDEATALLVGDALQSLAFQLLGEYRLAEDMKTQLEMVKLLAAATGSRGMA
                     GGQAIDLESVGKHLTVPELEFMHIHKTGALIRASALLGARCGNGLAQEEFGQLDLFAK
                     LIGLAFQVVDDVLDAESSTATLGKTAGKDAKDGKPTYVSAIGASRARELAQELRRNAL
                     DALAGFGDRALRLRQLADFIVLRKF"
BASE COUNT          395 a          719 c          794 g          399 t
ORIGIN      
        1 ccttgagcag cgccatgtac tcctcgaacc ggcctttcga gaccagcatg ctgccgaagc
       61 gctggatcgg catgttggtg agcacgtaga gccggttgaa aaccaccccg taccgttctc
      121 cgaggtggcg atggtaatcg gcttcgagct tgtcctgcgc cggcggcagg ctcggccctt
      181 gcgggttgta gaccaggttc aaggcgagcc ccgatccttc cttgccgtag ccgagcgcgt
      241 tgagcgtctt gagcgcgcgg atgctcgcct cgaacacgcc cttgccgcgc tgggcgttca
      301 cgttttcctc gaggtagcag ggcagcgaag cgacgacctc gacccggttg ccggcgagga
      361 actgcgccgt gtcctcctgg cccggctgct ccagcacggt gaggttgcag cggtcgatga
      421 cgtggacagc gaggttgcgc gcggccagca cgagcgaacg gaagtagggg ctgagttccg
      481 gcgcaccacc cgtgatatcc agcgtattga cacccgacgc cctgaggaat gagacaacct
      541 cggagacggt cttccagtcc atcatctccg tgcgcgtggg tcccgcgttg acgtggcagt
      601 gcacgcaggt ctggttgcat ttgtagccca ggttgacctg cagtgtttcc agtgaccggc
      661 ggcggatcgc cgggaagtcg cttacttcaa gccgtggcag ggtggcgtgc attcatcgct
      721 ccggggtgac ggcaggccgg tttcaaggca tggatggtga atcggattgt atcgtttgcc
      781 gctgcactgt atcgtgcagg ctctccagtt gcggcagcgc agggcgacat ggaggttgat
      841 acgagccggc agtgtattca gatgccagtg aagaagccgt gaaatcgcca tccgggccgc
      901 tgtagctcgt tgaccgcaac cctttgactg cgctactctt tcgccttcgt ccgaaagggg
      961 cggccaaccc gtgtccaagc cgtcgaaatc ccagccaccg caatcgctcg aggccgcact
     1021 ggcggaactc gagaagatcg tttcgagcat ggaggacggc cagctgccgc tcgagcgctc
     1081 gctcgcggct tacaagcgcg gcgccgagct gctcaagtac tgccagtccg ccctgcagga
     1141 tgcgcagcag caggtgaaag tgctggagaa cggcatcctg cgggacttca agggtgacga
     1201 caacgacaac gactgacttc tccgcctggt cgcacgccca tctggagcgt gtcgaggcgg
     1261 cgctgcagcg cgcgttgcca gcgccggaga tcgcgccgca gcgcctgcac caggccatgc
     1321 gctacgcggt gctgggaggg ggcaagcgcg tgcggccgct gctcgcattc gcggcgggcg
     1381 agcttgcgaa tgcggacccc gagcgcgtgg cgatagccgg cgcggccgtg gagctgatcc
     1441 acgcctattc cctggtgcac gacgatctgc cgtgcatgga cgacgacatg ctccgccgcg
     1501 gacggccgac ctgtcacgtc gaatacgacg aggcgaccgc gctgctggtg ggtgatgcgc
     1561 tgcaaagcct cgcgttccag ttgctcggcg aataccggct ggcggaagac atgaaaacgc
     1621 agctcgagat ggtgaagctg ctcgccgcgg ccacgggctc gcgcggcatg gcgggcgggc
     1681 aggcgatcga cctggagtcc gtcggcaagc acctcaccgt gcccgagctc gagttcatgc
     1741 acatccacaa gaccggcgcg ttgatccgcg cctcggccct gctcggcgcg cgctgcggca
     1801 atggcctcgc gcaggaggag ttcggccagc tcgatttgtt cgccaagctg atcgggctcg
     1861 cgttccaggt ggtggacgac gtgctcgacg ccgagtcctc caccgcgacc ctcggcaaga
     1921 ccgcgggcaa ggacgccaag gacgggaagc ccacctacgt cagcgcgatc ggcgcctcgc
     1981 gcgcgaggga gctcgcgcag gagctgcggc ggaatgccct cgacgcgctc gcgggcttcg
     2041 gggatcgcgc gctgcggctg cgccagctcg cggattttat tgtgctgcgg aagttctaac
     2101 tcctgttaaa gccggacgct tcgcgccggt ttaactccgc gcaatcgagc gggcgggtgc
     2161 ccccgccgct cattgcgcgg ataattggca atcggctaga gcagtggcaa cgggtatgta
     2221 gatgaccgag aacgtgatca attcgggaat gggcggcaag caggcggcgg ggacggccgg
     2281 gtacgagctg ctgaagagcg tgaacta
//
LOCUS       DBNGWZ010000668        25885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3143011, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000668 DBNGWZ010000000
VERSION     DBNGWZ010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 25885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 25885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25885
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3143011"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="AC16CX_20760"
     CDS             <1..980
                     /locus_tag="AC16CX_20760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR3014973.1"
                     /translation="DAARALPWPRLFDVRRSIRVNVSAIKSPVKSLDFVTLRVKDAVC
                     DIFRAERGKRPDVNTQSPDVRIHAFLTRDEVTFYLDTSDEALFKRGWRTAGGEAPLRE
                     NLAAGILKLSGWAAPTVLYDPMCGSGTFLVEAAMMALDVAPGEGRSFGFEKLENFDEK
                     MWRNLRDKARAQRKPARLLPIHGSDKSGTVLGLARENLAAAGLADAVQLKQMDILDGS
                     PPAESGIVVMNPPYGERQAEKCNLAAFYPKLGDVLKQRYAGWTAYIFSADMQLPRLIG
                     LKASKRTPLYNGALECRLFEYRLVAGSLRKKKPLTAGDAEDAEENKGKN"
     gene            complement(1017..1139)
                     /locus_tag="AC16CX_20765"
     CDS             complement(1017..1139)
                     /locus_tag="AC16CX_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014974.1"
                     /translation="MLDPNLIKAPPLELWPVIVRRVNVPEDVEAWLIRPIESVM"
     gene            complement(1356..2336)
                     /locus_tag="AC16CX_20770"
     CDS             complement(1356..2336)
                     /locus_tag="AC16CX_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR3014975.1"
                     /translation="MRASTARRRFLHLAGAAVATAAFPRLASAQGWPRRPIRAIVPFS
                     VGSSIDIISRIVLEPLAAQLGQPIVVENRGGAGGTIGAHLAAQAEPDGHTILIHASAH
                     TLTPAVYPKAPYDTEKAFAAVASFGSVPNVIMISPKKGIRTLQELAAAGRKGSVSYSS
                     AGVGSASHWAAERFRLSAGFDAVHVPFKGGPEAIIEVVTGRIDFVAPGLSVSLPFVRD
                     GRLLALAVSTPKRSTALPEVPTTLEAGFRDSDYTFWNGMFVPAKTPRAIVERLHREVQ
                     KALALPGVKEKFATQGIEPMPLRPAEFDALIRREIASNLAVAKAAKLKFD"
     gene            complement(2443..3384)
                     /locus_tag="AC16CX_20775"
     CDS             complement(2443..3384)
                     /locus_tag="AC16CX_20775"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3014976.1"
                     /translation="MKSSYAGIVGAPVTPFKADNSVDLETFARQVNFLIESGVRILAH
                     PMHIGESVNLTDGERGELARVLVSAAAGRVPTFVHVSHAGTDLAADLACRSMKAGATG
                     VVLMAPYHWRSGPEAILDHFLSVTGACGGKLIAYNNPSATGVELTPPVLEEMLDRIPG
                     FVAIKDATFHMETFTDICALIARRGKTVAAYTGIEYLLTSVPVGGSGCFSACSEIAPR
                     LVQDLYQACVRADLEKARELQYKVRRLLKLAMHNYPATIKYAMELMGRPVGVTRKPIQ
                     PLTEDEKGRVKAELTLLGVIETEPRGWNVAGANQAAA"
     gene            3652..4104
                     /locus_tag="AC16CX_20780"
     CDS             3652..4104
                     /locus_tag="AC16CX_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3014977.1"
                     /translation="MINDEEEVAMEREQTLRILNALANGVHPATGEKFAADSPYQHPD
                     TVRALFEAMRVVEGGAGPAPAPVPERKPAPPPQSGSGSRWSSEEEQRLVAAFDSGKTV
                     DELARAHSRSRAAIEARLVRLGKMDASAVTTPLRYPPKPAAPQARQGT"
     gene            complement(4616..5752)
                     /locus_tag="AC16CX_20785"
     CDS             complement(4616..5752)
                     /locus_tag="AC16CX_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HIR3014978.1"
                     /translation="MERDQAVKFMHDLLKAMVAKKASDLFITVGFPPAIKLDGKMTPV
                     ANQQLTPQHTSDLVRAIMNDKQASEFEASKECNFAISPGGIGRFRVNAFVQQGRIGMV
                     LRTITTTIPKFEDLNLPPVLKDIVMTKRGLMIMVGGTGSGKSTSLAAMVGYRNENSYG
                     HIITIEDPVEYTHDHKNCVITQREVGVDTESWHAALKNTLRQAPDVILIGEIRDRDTM
                     DYAIAFAETGHLCMATLHANSANQALDRVINFFPEERRHQLLMDLSLNIRAIVSQRLI
                     PKKDGKGRVAAVEVMLNSPLISDLIFKGEVHEIKEIMKKSRELGMQTFDQHLFDLYEA
                     GQISYEDALRNADSLNELRLMVKLHGKEAKEKDLTTGIEHLNIV"
     gene            complement(5791..6831)
                     /locus_tag="AC16CX_20790"
     CDS             complement(5791..6831)
                     /locus_tag="AC16CX_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3014979.1"
                     /translation="MDISELLAFVVKNKASDLHLSSGLPPMIRVHGDIRRINLPAMEH
                     KDVHSMVYDIMNDGQRKFYEENLECDFSFAIPNLARFRVNAFVQNRGAAAVMRTIPSK
                     ILSLEDLKAPKIFADLADQPRGVVLVTGPTGSGKSTTLAAMINHKNENEHGHILTVED
                     PIEFVHESKKCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMMRAMLSESLRAVISQALLKTKDGSG
                     RVAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGQQYGMQTLDQNLQDLVKRNVISTEE
                     ARTKAANKDNFK"
     gene            6938..7735
                     /locus_tag="AC16CX_20795"
     CDS             6938..7735
                     /locus_tag="AC16CX_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR3014980.1"
                     /translation="MATIAANLQAVRARIARAAQAAGRRADDIVLVAVSKTFPPERIV
                     EAHAAGQRVFGESHAQEAVQKITELARLPLEWCVAAAHREDDRIRSDAPVPSAGVASL
                     EGPLLLPLEWHFIGPIQSNKTGVIAAHFSWVHSVDRGKIAQRLNAARAEGMAPIDVCI
                     QINVSGETGKSGVRPGEEESLAAAIARLPRLRLRGLMAIPEPTSDTRLQRRRFALLRE
                     LKDKLVARGYALDTLSMGMSDDFEAAIAEGATMVRIGTAIFGPRKNL"
     gene            7878..8690
                     /gene="proC"
                     /locus_tag="AC16CX_20800"
     CDS             7878..8690
                     /gene="proC"
                     /locus_tag="AC16CX_20800"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3014981.1"
                     /translation="MNITFIGGGNMASAIIGGLIRKRWPRKAIRVVEIAPAARERLER
                     EFKVKTHAALDGAAARSDCIVLAVKPQQMREVAAALKPRLASQLVVTIAAGIRVKDLS
                     RWLGGYPRIVRVMPNTPALVHAGISGLYAPVAVGASDRAAAGRILGAVGRILWLEREE
                     GLDAVTAASGSGPAYVFYFIEALERAARELGLTAEAAHQLAIETFSGAVRLAAQSSEP
                     PETLRSRVTSKGGTTERALKSMEADRVKDAIVRAVRAAAERSRELGDELGKD"
     gene            8808..9383
                     /locus_tag="AC16CX_20805"
     CDS             8808..9383
                     /locus_tag="AC16CX_20805"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3014982.1"
                     /translation="MVTGALIFLVETGFGLFVVALLLRFYLQWVRAPYRNPLSEFLQA
                     LTDFMVRPARRLVPGLWGLDLATLALAWLIQLLELFIVMQLRGYQAGPDAGIAFAALF
                     VLALVALVRIGLYIVMAAVIVQVVLSWVGPYSPVLPLLNSMTRPFLRVFQRRIPPVGN
                     VDLSPLFVLVVIQLLLILLIAWVEAALTRLL"
     gene            9410..9733
                     /locus_tag="AC16CX_20810"
     CDS             9410..9733
                     /locus_tag="AC16CX_20810"
                     /inference="COORDINATES: protein motif:HMM:NF014633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3014983.1"
                     /translation="MTGDSSWYRYDAAARRFSLTLHVQPNARKTAFAGLHGNALKVKI
                     AAPAVDNKANAELVGFLSEALGVPKSTIAIRHGVTGRRKIVEIAAGPEIAARLEALAG
                     GRAEH"
     gene            complement(9806..11266)
                     /gene="rng"
                     /locus_tag="AC16CX_20815"
     CDS             complement(9806..11266)
                     /gene="rng"
                     /locus_tag="AC16CX_20815"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HIR3014984.1"
                     /translation="MAVTEEILVNVTPQETRVAVIEQGVTQEVHVERASSRGLVGNIY
                     MGRVIRVLPGMQSVFIDVGGERAAFLHVADMRGHRRNGGSAAPIEKLLGEGQNLMVQV
                     VKDPIGTKGARLSTQVSIAGRFLVYLPQDSHIGISQRIEDEEERTHLREKLQLLLPPE
                     EKGGFIIRTVAETASDRELKSDIDYLRRLWGGIQDKAAAAAPLTPLYQDLNLSLRVLR
                     DFVHEDTARVLVDSRETCRSMLEFGRDFTPNVAERIEHYSGERPLFDLYGVEEEIEKA
                     LARRVELKSGGYLIIDQTEALTTIDVNTGGFVSGRSFDDTIFKTNLEAAQVIARQLRL
                     RNLGGIVIIDFIDMDTEEHRNAVLNEFRKTLARDRTHMTVNGFTQLGLVEMTRKRTRE
                     SLAQVLCEPCPVCEGRARLKTAQTVCYTILRELTREARQFNAREFRILASQPVIDLFL
                     DEESQSLAMLSDFIGKPVSLQVETAYNQEQYDIILM"
     gene            complement(11280..11900)
                     /locus_tag="AC16CX_20820"
     CDS             complement(11280..11900)
                     /locus_tag="AC16CX_20820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR3014985.1"
                     /translation="MALSDQRIYLASRSPRRRDLLKQIGVPFELLLLREDLRRGADVD
                     ETPLADESPGVYVLRIARAKVEMAVRQIAYRGLPQKPVLAADTTVVFDGQIVGKPEDA
                     EHAARILRALCGREHQVLSAVAIALREQVETQISVSSVWFREIPDADIRRYCASGEPL
                     DKAGAYAIQGRAGSFVTRISGSYSGIMGLPLAETVELLQKFNISVF"
     gene            complement(12006..12470)
                     /gene="rlmH"
                     /locus_tag="AC16CX_20825"
     CDS             complement(12006..12470)
                     /gene="rlmH"
                     /locus_tag="AC16CX_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR3014986.1"
                     /translation="MKFLVVAVGHRMPAWVDAGFAEYAGRMPREARIGLVALKPAARG
                     GPVKRALETEGERILAALPRGCVRVALDERGTLIGTMDLARRIARWRETGRDVAFIVG
                     GADGLAESVKESSDFVWSLSPLTLPHGLARVVLAEQLYRAVSILHNHPYHRE"
     gene            complement(12745..13101)
                     /gene="rsfS"
                     /locus_tag="AC16CX_20830"
     CDS             complement(12745..13101)
                     /gene="rsfS"
                     /locus_tag="AC16CX_20830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR3014987.1"
                     /translation="MTLDKLAKAAVAALEDIKGRDIVVLDARKMTSLFDKIIIASADS
                     TRQSKALSNNLQEKMKALGARIHGVEGERAGEWILVDLGAIVVHIMQPAIRKYYNLEE
                     LWAPPRSRRRRAAGAR"
     gene            complement(13109..13765)
                     /gene="nadD"
                     /locus_tag="AC16CX_20835"
     CDS             complement(13109..13765)
                     /gene="nadD"
                     /locus_tag="AC16CX_20835"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR3014988.1"
                     /translation="MSTAPIGVLGGTFDPIHYGHLRLGEELGETLRLGEVRFVPSGIP
                     PHRSAPQVAAEHRLAMTRLATADNPRFRVDEREVRRAGPGFTFDTLTELRAEAGASRP
                     LVLLLGADAFLEFATWHRWHEIFGLAHVAVAHRPGFPVERWAERMPQPLAREYTARLM
                     QQPLATHLQPAGGIVVVPFTALDLSATAIRDMVRSGASPRYLLPAEVLDYIRANRLYS
                     "
     gene            complement(13882..15192)
                     /gene="aceF"
                     /locus_tag="AC16CX_20840"
     CDS             complement(13882..15192)
                     /gene="aceF"
                     /locus_tag="AC16CX_20840"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HIR3014989.1"
                     /translation="MANVIEVKIPDIGGFKDVPVIEVFVKPGDAVKPEDSLVTLESDK
                     ATMDVPSPAAGVVKELKVKIGDKVSEGSLVLTLESPGDGASAKSPAQKPAPVPAQQPA
                     PRAAPAPSPAPAAAPQPPPSPPLGKGGVDEAGFARAHASPSIRRFARELGVDLARVRG
                     TGPKERVLKEDIQSYVKGELSRPRAAEGGLALSLPPLPQVDFAKFGPVTNQPLSRIKK
                     LSGANLHRNWVTIPHITQHDEADITELEEFRKIQVEEAKKQGIRFTILCFLLKAVVVA
                     LKQHPEFNASLSADGESLVLKQYFHIGVAVDTPNGLVVPVIRDVDKKGLMELAKELGE
                     VSARMRAGKISPADLQGGCFSISSLGGLGGSNFTPIINAPEVAILGVGKSVTRPMWNG
                     SAFMPRLMLPLSVSYDHRVIDGAQGARFIAFLSSVLSDIRRLVL"
     gene            complement(15344..18019)
                     /gene="aceE"
                     /locus_tag="AC16CX_20845"
     CDS             complement(15344..18019)
                     /gene="aceE"
                     /locus_tag="AC16CX_20845"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR3014990.1"
                     /translation="MDLRDIQPDFDPQETREWLEALDSVLEREGPARANFLLDRLVEK
                     ARSHGAYIPYNANTAYMNTIPMSQEERSPGDAELENKIRSLVRWNAMAMVLRANKESS
                     ELGGHIASFASAATLYDVGFNHFFRAWNDHFGGDLVYFQGHSAPGIYARAYLEERVTE
                     EQLKHFRQEVGGKGLSSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFMRYLKDRGIV
                     EPQGRKVWCFCGDGEMDEPESLGAISLAGREQLDNLIFVINCNLQRLDGPVRGNGKII
                     QELESAFRGAGWNVIKVIWGSYWDPLFMRDKTGILLRRMEECVDGEYQAFKSQDGAFV
                     RKHFFGKYPELLEMVAHMSDDDIWRLNRGGHDPHKVYAAYAAAMKHKDQPTVILAKTI
                     KGYGMGEAGEGQNITHQQKKMGTSSIKAFRDRFNVPIPDDKLEEVPFYRPPEDSPEMK
                     YLRERIQAMGGSVPARRRKSQPLEVPPLSAFEAQLKGTDGREISTTMAFVRILTTIIR
                     DKGIGKLVVPIVPDESRTFGMEGMFRQLSIYSHVGQLYTPQDADQLMYYKEDRNGQIL
                     QEGICEAGAMCSWIAAATSYSTHNQPMIPFYIFYSMFGFQRVGDLAWAAGDMRARGFL
                     LGGTSGRTTLNGEGLQHEDGHSHLLASTIPNCVSYDPTFAYELAVIIQDGLRRMYREQ
                     EDVYYYVTVMNENYAHPAMPEGVEKGILRGMYPLSRGKAAKNKLRVQLMGCGTILREV
                     IAAAEMLEKDFGVSADIWSVTSFNELRRDGVETARWNLLHPEAEPRRSYVEQCLRDHP
                     GPVVAATDYMKVFADQIRAYLPQPAHYSVLGTDGFGRSDTRRALRGFFEVDRRYVAVA
                     ALKALADQEALPQKKVVEAIKKYGVDPEKPNPAKV"
     gene            complement(18200..18985)
                     /locus_tag="AC16CX_20850"
     CDS             complement(18200..18985)
                     /locus_tag="AC16CX_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3014991.1"
                     /translation="MAFQNILLEQPEAGIYLLTVNRPKALNALNAATLDEIASAVKKV
                     AADDKARVLLVTGAGEKAFVAGADIAEMQNATIEQAREFSEKGMRVMHALEALPVPVI
                     ALVNGYALGGGCELALACDWILASEAAVFGQPEVNLGIPPGFGGTQRLPRRIGPARAL
                     ELLTTARQVKAEEALAIGLANHVCPASGLRAKGLEMAHAIASKGPIAVRLAKQAVQRG
                     ANLDLFSACYLETDLFVQAFTTQDRMEGMNAFLEKRPAKFQGH"
     gene            complement(19277..20044)
                     /locus_tag="AC16CX_20855"
     CDS             complement(19277..20044)
                     /locus_tag="AC16CX_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011657744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HIR3014992.1"
                     /translation="MQIAGHTFLVTGGASGLGAATARRLTHAGGNVALCDLDAGQGKQ
                     LALEIGAHALFVPMDVTDEARVTQAIAAARGKFGALHGAVSCAGIAPGERVLGKTGPH
                     SLDTFRRAIEINLIGTFNVLRLAAQAMAENQPDAEGERGIIINTASIAAYDGQVGQAA
                     YSASKAGVAGLTLPAARELARFGIRVMTIAPGIFETPMVAAFAPELKQSLAAQVPFPS
                     RLGKPEEYAMLVEHIIVNPMLNGEVIRLDGALRMPAK"
     gene            complement(20141..21109)
                     /locus_tag="AC16CX_20860"
     CDS             complement(20141..21109)
                     /locus_tag="AC16CX_20860"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014993.1"
                     /translation="MKSKCLLTAAILVAVVPATLQAQSFPAKPVRIIIPFAPGGNVDL
                     VVRGIAPRLSEALGQQVIPDNRPGSSGLLGTHLVAKAAPDGYTLLAMANTFAVVPSIM
                     SNPGYDPLKDFVGVSQTCLVPQVLVVNTSLPVRSVKQLVALAKARPGEMSYASAGVGG
                     TGHMAAELFNRHAGVKMLHVPYKGNAQAIVDVISGQVTMMFDQVSTSTPHIKAGKLRG
                     LAVTSLKRSPLLPDLPTIDESGMRGFEDITFNGLVAPAGTPREILARLNADVAKAVAA
                     PEPRQRFIQMGVELTASASPEAFMEYIRAEFVKKAKLAREAGIKIE"
     gene            complement(21147..22823)
                     /locus_tag="AC16CX_20865"
     CDS             complement(21147..22823)
                     /locus_tag="AC16CX_20865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR3014994.1"
                     /translation="MRGMGDELGIAQAFLQAWARLLSDPFKLAEAQLRYLQDTLSLWQ
                     SSMLKLMGQDAKPVAEPRQGDRRFQHEDWQQNFLYDYIKQSYLIAARHLHQTLGKVEG
                     LDEQTARKVEFYTRQYIDALSPSNFLHTNPEVLRETVASGGHNLVKGLNNLLEDLSRG
                     GGEQIQLRMTDENAFKLGVNIATTPGKVVFQNDLIQLIQYFPASGPVHRRPLLIIPPW
                     INKYYILDLREANSFVKWAVEQGHTVFAISWVNPDKRLAHKRFDDYLAEGTLAALDAI
                     EKATGESEVNAIGYCLGGTLLACTLGYLAAKKDERIVSGMFFASMIDFEEAGELEVFI
                     DEKQVEALERRMLERGYLEGSEMATTFNLLRANDLIWSFVVNNYLLGRDPFPFDLLYW
                     NSDSTRMPATMHSYYLRNLYLRNLLAKPGGITLGGVPIDLTRVKAPLYFVSTLEDHIA
                     PWRSTYAGAKLFTAPVRFVLGGSGHIAGIINPPAAKKYSYWTNPKLPGQPDQWLKGAS
                     QHPGSWWPDWAGWVAQHGGDKVPACIPGEGRLKAIEDAPGAYVKMRADKG"
     gene            23038..23442
                     /locus_tag="AC16CX_20870"
     CDS             23038..23442
                     /locus_tag="AC16CX_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3014995.1"
                     /translation="MSTTYTISDLAREFDVTTRTIRFYEDQGLLSPKREGTSRIFSNR
                     DRVRLKLALRGKRLGFSLAEIRELFELYDLSRDEREQLEEFLGKLERRRAHLEQQRED
                     IEVMLNEINFFANQCKRLLKDKPASEDTKPTT"
     gene            23690..24850
                     /locus_tag="AC16CX_20875"
     CDS             23690..24850
                     /locus_tag="AC16CX_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HIR3014996.1"
                     /translation="MLQGLNFELGETVDILRETVSSFAAREIAPRAAETDRSNEFPRD
                     LWPKLGKLGLLGITVEEEYGGAGMGYLAHVVAMEEISRGSASVGLSYGAHSNLCVNQI
                     RRNGSAEQKRRYLPKLVAGEHVGALAMSESGAGSDVVGMRLRAQKRGDRYVLNGTKMW
                     ITNGPDADVLVVYAKTDPAAGARGITAFVIEKSFKGFSTAQKLDKLGMRGSNTCELVF
                     SDCEVPEENVLGAAGKGVEVLMSGLDYERVVLAAGPLGIMQACFDVVLPYLHERRQFD
                     QPIGEFQLMQGKLADMYVTLNACRAYVYAVGRACDRGEVTRKDAAGAILFASEKATWM
                     TLEAIQCLGGMGYINDTPTGRLLRDAKLYEIGAGTSEIRRWLIGRELYDETR"
     gene            25458..>25885
                     /locus_tag="AC16CX_20880"
     CDS             25458..>25885
                     /locus_tag="AC16CX_20880"
                     /inference="COORDINATES: protein motif:HMM:NF015047.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR3014997.1"
                     /translation="MAKLHTPLCDRLGIRLPIIQAPMAGGASSTELVAAVSRAGALGS
                     FGFAYTQPESMQRDAEAVRAHTHLPFNLNLFASQQPGAIDPAAQRGAIDAVAGYYAEL
                     GLPPPEPARPPYAPDLEAQFAAAEEIRPPVLTFHLGVMQH"
BASE COUNT         4504 a         8248 c         8307 g         4826 t
ORIGIN      
        1 tcgacgccgc gcgcgcgctt ccctggccgc ggctcttcga cgtgcgccgc tcgatccgcg
       61 tcaacgtctc cgcgatcaag tctcccgtaa agagcctgga tttcgtcacg ctgcgcgtga
      121 aggacgcggt gtgcgacatt ttccgcgcgg agcgcggaaa acgtcccgac gtgaacacgc
      181 aatcaccgga tgttcgtatc catgctttcc tgacgcgcga cgaggtcacc ttctacctcg
      241 acacctcgga cgaggcgctg ttcaagcgcg gatggcgcac ggccggcggc gaggcgccgc
      301 tacgcgagaa cctcgccgca ggaatcctga aactttcggg ctgggcggcg ccgacggtgc
      361 tctacgaccc catgtgcggc agcggaacct ttctcgtcga agcggcgatg atggcgctcg
      421 atgtcgcgcc cggggaaggc agatcattcg gtttcgaaaa gctggagaat ttcgacgaga
      481 aaatgtggcg caacctgcgc gacaaggccc gcgcgcagcg caagcccgcg cggctgctcc
      541 cgatccacgg gagcgacaaa tctgggacgg tcctcgggct ggcgcgcgag aacctagcgg
      601 cggcggggtt ggcggacgcc gtgcagctca agcaaatgga catcctagac ggcagcccgc
      661 ccgcggagtc tggtatcgta gtgatgaatc ccccatacgg cgagcggcag gcggaaaagt
      721 gcaacctcgc cgcgttctac ccaaagctgg gcgacgtcct gaagcagcgt tacgcgggct
      781 ggaccgccta catcttctcc gcggacatgc agctacccag gttgatcggc ctgaaagcct
      841 cgaaacgaac gccgctctac aacggcgcgc tcgagtgccg gctgttcgag taccggctgg
      901 tggcggggag cctgcggaaa aagaagcctc ttaccgcagg ggacgccgag gacgcagagg
      961 aaaataaggg caagaattga accgcggaga cgccgagaga aacagggaaa cgatatttac
     1021 atcaccgatt cgatcgggcg gatgagccac gcctcgacgt cctccggaac attgacgcgg
     1081 cgcacgatca ccggccacag ctcgagcggc ggcgctttga tcaaattggg atcgagcatt
     1141 acaacgtcaa gatcgtcgtg caggtagggc ctgcgcccgc aatggcgtgc gtcgcaagac
     1201 acccggatcc ccgggcggca ttccctccag tggcaggtcc ggccgatgta attgccggat
     1261 aagtcggcga ataaataggg tcagtgtaac ttttctctgt cacttcggcc cactctgcac
     1321 gaaaagttac actgacccta tttatcgact tgacgtcaat cgaatttgag tttggctgcc
     1381 ttggcgaccg cgaggttgct tgcgatttcc ctgcggatga gcgcgtcgaa ttcggcgggc
     1441 ctgagcggca tcggctcgat tccctgcgtg gcgaacttct ccttcacgcc gggcaaggcc
     1501 agcgctttct gcacttcccg atgcagccgc tccacgatcg cgcgcggcgt cttcgcgggc
     1561 acaaacatcc cattccagaa cgtgtagtcg gagtcgcgaa agcctgcctc cagtgtcgtt
     1621 ggcacttcgg gaagcgcggt cgagcgtttg ggcgtgctca ccgccagcgc gagcaatctg
     1681 ccgtcgcgaa cgaacggcag cgagaccgac aggcccggtg ccacgaaatc gatgcgcccg
     1741 gtgaccacct cgatgatggc ttccgggcca cccttgaacg gcacgtgaac ggcatcgaat
     1801 cccgcgctca gccgaaaacg ttccgccgcc cagtggctcg cgctgccgac gcccgcggag
     1861 gaatagctga ccgatccctt tctgccggcg gccgccagtt cctggagcgt ccggatgccc
     1921 ttcttgggcg agatcatgat gacgttgggg acgctgccaa acgacgcgac cgcggcaaaa
     1981 gccttctccg tgtcgtaggg cgctttcgga tagaccgccg gggtcagggt atgcgcggag
     2041 gcgtgaatca ggatcgtgtg accatccggc tccgcttggg cagccagatg ggcgccaatc
     2101 gtgccaccgg cgccgccccg gttctccacc acgatcggct ggccgagttg ggccgcgagc
     2161 ggctcgagca cgatgcgcga gatgatgtca atcgagctgc caaccgaaaa cggaacgatg
     2221 gcgcggatgg gccttctcgg ccacccctgt gccgaggcga ggcgcgggaa agccgccgtc
     2281 gcaacggcgg ccccagccag gtgcaggaag cggcggcgtg cagtcgaggc tctcataccg
     2341 ggctccttca aatgcgacgt cgctttggtg acgagcgaga atgccgtctg cggcacggtc
     2401 tggattgcgc ggattatgcg aggcgatcgt gccccggtca attcaggctg ccgcctgatt
     2461 cgcgcccgcg acattccagc cgcgcggttc agtctcgatc acgcccagta gcgtgagctc
     2521 cgcctttact cgcccctttt cgtcttccgt cagcggctgg attggcttgc gggtcacccc
     2581 caccggccgc cccatcagct ccatggcgta tttaatggtc gccggataat tgtgcatcgc
     2641 gagcttgagc agacggcgca ctttgtactg gagctcgcgc gccttctcca gatcggcgcg
     2701 cacgcatgcc tgatagaggt cctggacgag ccgcggggcg atttcgctgc acgccgagaa
     2761 gcagccgctg ccgccgaccg ggacggaagt caggagatat tcgatgccgg tgtacgcggc
     2821 aacagtcttg cccctgcggg cgatgagcgc gcagatgtcc gtgaaggtct ccatgtgaaa
     2881 cgtggcgtcc ttgatggcga cgaagcccgg gatccggtcg agcatttcct cgagaacggg
     2941 tggcgtcagc tcgacgccgg tggccgaggg attgttgtag gcgatgagct tacctccgca
     3001 ggcgcccgtg accgacagaa agtgatccag gatcgcctcg ggccccgaac gccagtggta
     3061 cggcgccatc agcacgacgc cggtcgcgcc ggccttcatg ctgcggcacg ccagatcggc
     3121 cgcgaggtcg gtgcctgcat gggacacgtg gacaaaagtc ggaacacgtc cggccgctgc
     3181 ggaaacgagc acgcgcgcaa gctccccgcg ttcgccatcc gtcagattga cggattcccc
     3241 gatgtgcatc ggatgcgcga gaatccgcac gccgctctcg atcaggaagt tgacctgcct
     3301 tgcgaaggtc tcgagatcga ccgagttgtc ggccttgaac ggggtgaccg gcgcgccgac
     3361 gatgccggca tagctggatt tcatggttcc gccccttgcg attgcaaacg atgccctgat
     3421 tagacatcgc gcgggcattg ctgtccaacg ccagcgttgg ctgggccaca cccggaatct
     3481 caagcgttag tgctccacaa cacgcggccc atcaattcat ggggtgctct tttcctgtag
     3541 tccagaaatt actgctcggc gttcttggcg ccttggcggc taatttcaaa cctcgtcaac
     3601 cggcgcgtga gctgccgcgt tgagcagtca cgcgggccgg caggcggtcc gatgatcaac
     3661 gacgaggagg aagtagccat ggagagggaa cagacactca ggatactgaa cgccctggcg
     3721 aacggcgttc acccggcgac gggcgagaag ttcgcagccg acagcccgta ccagcacccc
     3781 gacaccgtgc gggccttgtt cgaagccatg cgcgtggtcg agggaggcgc tggcccggct
     3841 ccggcgccgg tccccgagcg aaaacccgcg ccgccgcccc agagcggctc cggctcgcgc
     3901 tggagcagcg aggaggagca acgacttgta gccgcattcg attcgggcaa gacggtggat
     3961 gagctcgcgc gcgcccacag ccgcagccgc gcggcgatcg aggcgaggct ggtgcggctc
     4021 ggcaagatgg atgcttcggc ggtcaccacg ccgctgcgct atccgccgaa acccgcggcg
     4081 ccccaggccc ggcagggcac ctgaccacta aacccgtgta accctcgacg caaaggcgca
     4141 aagccgcaaa gggaagactc gacttccatt ttcagaaagg actttgcgtc tttctttttt
     4201 tctttgcggc ctttgcgttg agtttttttg aagcggggct agccgcccgc cagcgcgcgt
     4261 cgcgcggaac gcgagcttgt cctgcaggcg gtgcgacagg cagatcgcat acggcggcgc
     4321 gccgacaacg agcgcgcgag atcgaacggg acggtcatgg gcagaaaaaa ggcgccggca
     4381 ccatgccgac gccctttcca tttcccacgc cagttgaacg gctgcttgca gtccgctcga
     4441 accggcgtat ctgacccggc gcgcagcata cggtagaggc attgaggcga taagtcatat
     4501 gaaaaaagag cgtcggacct gccaccgacg ctcttctgtg aattgcgccg aatgaacggc
     4561 gagctacttc gtccgttcga ttcggcgcat ctgacccggc gcgcagcgtc cggtgtcaga
     4621 cgatgttgag atgctcgatg cccgtcgtga gatccttctc cttcgcctcc ttgccgtgga
     4681 gcttgaccat caggcgcagc tcgttgagcg agtcggcgtt gcggagcgcg tcctcgtagc
     4741 tgatctgccc cgcctcgtag agatcgaaca ggtgctggtc aaaggtctgc atgccgagtt
     4801 cgcgcgattt cttcatgatc tccttgatct cgtgcacctc gcccttgaag atgaggtcgg
     4861 agatcagcgg ggagttcagc atgacctcga ccgccgccac ccgccccttc ccgtccttct
     4921 tcgggatcag gcgctgcgag acgatggcgc ggatgttgag cgagaggtcc atcagcagct
     4981 ggtgcctgcg ctcctccggg aagaagttga tgacgcggtc gagtgcctgg ttggcgctgt
     5041 tggcgtgcag cgtcgccatg cacaggtggc cggtctcggc gaaggcgatg gcgtagtcca
     5101 tggtgtcgcg gtcgcggatc tcgccgatca ggatcacatc cggcgcctgg cgcagggtgt
     5161 tcttgagcgc ggcgtgccag gactcggtgt ccaccccgac ctcgcgctgc gtgatgacgc
     5221 agttcttgtg gtcgtgcgtg tactcgaccg ggtcctcgat ggtgatgatg tggccgtagc
     5281 tgttctcgtt gcggtagccg accatcgccg cgagcgaggt ggacttcccc gagccggtgc
     5341 cgccgaccat gatcatgagg ccgcgcttgg tcatcacgat gtccttcagc accggcggca
     5401 ggttcaagtc ctcgaacttc gggatcgtgg tggtgatggt ccgcagcacc atgccgatcc
     5461 gcccctgctg cacgaaggcg ttcacccgga aacgcccgat gccgccgggg ctgatcgcga
     5521 agttgcactc cttgctcgcc tcgaactcgc tcgcctgctt gtcgttcatt atggcgcgga
     5581 caaggtcgga cgtgtgctgc ggcgtgagct gctgattggc gaccggcgtc atcttgccgt
     5641 cgagcttgat cgccggcggg aatcccaccg tgatgaagag gtccgacgcc ttcttcgcga
     5701 ccatcgcctt cagcaggtcg tgcatgaatt tcactgcctg gtctcgttcc atacgctgct
     5761 cctacgttga attccggatt gtgccgcggc ctatttgaaa ttgtccttgt tggccgcctt
     5821 ggtgcgcgcc tcttccgtcg agatgacgtt gcgcttcacc aggtcctgca ggttctggtc
     5881 cagcgtctgc atcccgtact gctggccggt ctggatcgcg gaatacatct gcgccacctt
     5941 ggcctcgcgg atcaggttgc ggatcgccgg cgtgccgacc atgatctcgt gcgccgcgac
     6001 gcgcccgctg ccgtccttgg tcttgagcaa cgcctgtgaa atcaccgcac gcagcgattc
     6061 cgacagcatc gcgcgcatca tttccttctc ctcggccggg aacacgtcga tgatccggtc
     6121 gatggtcttg gccgcggaac tcgtgtgcag cgtgccgaac accaggtggc cggtctccgc
     6181 ggcggtcatc gcgaggcgga tcgtctcgag gtcgcgcatc tcgcccacca ggatcacgtc
     6241 cgggtcctcc cgcagtgcgc tcctcagcgc gttagcgaag gacagggtgt gcgggcccac
     6301 ctcgcgctgg ttgatcaggc actttttcga ttcgtgcacg aattcgatcg ggtcctccac
     6361 cgtcaggatg tgcccgtgct cgttttcgtt cttgtggttg atcatcgccg ccagcgtcgt
     6421 cgacttgccc gatcccgtcg ggccggtcac cagcaccacg ccgcggggct ggtcggcgag
     6481 gtcggcgaaa atcttgggcg ccttgaggtc ctccagcgac aggatcttgg aggggatggt
     6541 ccgcatcacc gccgcggcgc cgcggttctg cacgaaggca ttcacccgga agcgcgcgag
     6601 gttggggatc gcgaacgaga agtcgcactc gaggttttcc tcgtagaact tgcgctgccc
     6661 gtcgttcatg atgtcgtaca ccatggagtg cacgtccttg tgctccatgg cgggcaggtt
     6721 gatgcgccgt atgtcgccgt gcacgcgaat catcggggga aggcccgagg acaggtgcag
     6781 gtcggaggcc ttgttcttga ccacgaatgc cagcagttcg gagatgtcca tttataattc
     6841 tccaaataac gtgatgagtg cggcgtgagg ggtggggagt aaatgataag gttctgattt
     6901 tacgcctgac acttcacgcc ccacacctcg caaaattatg gccacaatcg ccgccaactt
     6961 gcaagccgtg cgcgcgcgca tcgcgcgcgc ggcgcaggcc gccggcaggc gcgccgatga
     7021 catcgtgctg gtggcggtga gcaaaacctt cccccccgag cgcatcgtgg aggcgcatgc
     7081 cgccggccag agggtgttcg gggaaagcca cgcccaggaa gccgtgcaaa aaatcacgga
     7141 acttgcccgg ctgccgctcg aatggtgcgt cgcggcggcg catcgagaag atgatcgcat
     7201 tcgaagcgac gcgcctgtcc cctccgccgg ggtcgcatcc ctggaggggc ccctgctcct
     7261 ccctctggag tggcacttca tcggaccgat acaaagcaac aagaccggcg tgatcgcggc
     7321 tcatttttcg tgggttcaca gcgtggatcg tgggaaaatc gcgcaacgtc tcaacgccgc
     7381 gcgggcggag ggaatggccc cgatcgacgt ctgtatccag atcaacgtca gcggcgagac
     7441 cggcaaaagc ggggtcaggc ccggggagga ggagagcctg gctgcggcga tcgcacggct
     7501 gccgcggctg aggctgcggg gattgatggc gataccggaa cctacgtccg acacgaggct
     7561 ccagcgccgg cgcttcgcgc tgctgcgcga gctgaaggac aagcttgtcg cgcgtggcta
     7621 tgcgctggat acgctctcga tgggcatgtc ggatgacttc gaggcggcga tcgccgaggg
     7681 cgcgaccatg gtgcgcatcg gcaccgccat tttcggaccc cggaaaaacc tctgacgcaa
     7741 agtcgctaag ccgcaaaggt gtcaaaatat aatctcatag tggtcgcaca gcacctgata
     7801 ggcgattcgg gcggcaattt caccgcaaat tattcgtttt tctttgcgtc tttgcgtctt
     7861 tgcgtcaaat ggccaacatg aacataacct tcatcggcgg cggcaacatg gcgagcgcga
     7921 tcatcggcgg gctcatccgc aagcgctggc cgcgcaaggc gatccgcgtg gttgaaatcg
     7981 cgccggctgc ccgcgagcgg ctggagcgcg agttcaaggt gaagacccat gccgcgctcg
     8041 acggggcggc ggcaaggagc gattgcatcg tgctcgcagt gaagccgcag cagatgcgcg
     8101 aggttgcggc tgcgctcaag ccccgcctcg cctcgcagct cgtcgtcacc atcgcggccg
     8161 gcatccgggt gaaggacctc tcgcgctggc tgggcggcta cccgcgcatc gtgcgcgtta
     8221 tgcccaacac gccggcgctc gtgcacgccg gcatctccgg actctacgcg ccggtggcag
     8281 tgggcgcctc cgaccgtgct gcggccggga ggatcctggg ggcggtgggg agaatcctgt
     8341 ggctcgagcg cgaggagggc ctcgatgccg ttaccgccgc ctcgggtagc ggccccgcct
     8401 acgtctttta cttcatcgag gcgctggaac gggccgcgcg cgagctggga ctgactgcgg
     8461 aggccgcgca ccagctcgcg atcgagacct tctccggcgc ggtcaggctt gcggcgcagt
     8521 cgagcgagcc gccggagacg ttgcgcagcc gcgtcacctc caaaggcggg acgacggagc
     8581 gcgcattgaa gtccatggaa gcggaccggg tgaaggatgc gatcgtgcgc gcggtgcgtg
     8641 ccgcggccga gcgctcgcgc gaactgggcg acgagcttgg gaaggattga gtccgtaagg
     8701 cgtgaggagt gaggcgtgag gaggatcact acgaatcccc gttttttttc tttacacctc
     8761 acgcctgacg cctcccgcct cacggcgatg cgcgtccggg agggcgcatg gtgaccggcg
     8821 cgctgatctt cctggtcgaa accggcttcg ggctgttcgt cgtcgccttg ctgctgcgct
     8881 tctacctgca atgggtgcgc gcgccgtacc gcaacccgct ttccgagttc ctgcaggcgc
     8941 tgaccgattt catggtgcgg cccgcccgcc gcctcgttcc cggcctgtgg ggcctcgatc
     9001 ttgcgacgct cgcgctggcg tggctcattc agctgctgga gctgttcatc gtgatgcagc
     9061 tccgcggcta ccaggccggg ccggacgccg gcattgcgtt cgccgcgctg ttcgtgctgg
     9121 cgctggtcgc gctcgtcagg atcggcctct atatcgtgat ggccgcggtg atcgtgcagg
     9181 tggtgctgtc ctgggtaggc ccctacagtc cggtgctgcc gctgttgaac agcatgacgc
     9241 ggccttttct gcgcgtgttc cagcggcgga ttccgccggt cggcaacgtc gatctgtctc
     9301 cgctgttcgt gctcgtggtc atccagctgc tgctgatact tctgatcgcg tgggtggagg
     9361 ccgccctcac gcggctgctt tgattcctga tacgagtgaa tcgtgactcg tgactggtga
     9421 ctcaagctgg taccggtatg acgccgctgc gcgtcgcttc tcgctcacgc tgcacgtcca
     9481 gccgaacgcg agaaaaaccg cgttcgcagg attgcacggc aacgccttga aggtaaaaat
     9541 cgccgcgccg gccgttgaca acaaagccaa tgctgagctg gttgggtttc tgagcgaagc
     9601 gctcggggtc ccgaagtcca cgatcgccat ccgccacggc gtgaccggtc gccgcaagat
     9661 tgtcgaaatc gccgccggcc ccgaaatagc agcgcgccta gaggcgttgg cggggggtcg
     9721 agcagagcat tagagactca gaaatccgaa tgtcacgacc gtgacattcg ggtgaccagt
     9781 cacgagttac cagtcacgag tcacgctaca tcaagataat gtcgtactgc tcctggttgt
     9841 acgcggtctc gacctgcagc gacaccggct tgccgatgaa gtccgagagc atggcgagcg
     9901 attgcgattc ctcgtcgagg aagagatcga tcaccggctg ggaggcgagg atgcggaact
     9961 cccgcgcgtt gaactggcgc gcctcgcgcg tgagctccct caggatcgta tagcacaccg
    10021 tctgcgcggt cttgagccgg gcgcggccct cgcataccgg gcacggctcg cataatacct
    10081 gggcgagcga ctcgcgggtg cgcttgcgcg tcatctccac cagccccagc tgggtgaagc
    10141 cgttcaccgt catgtgggtg cggtcgcgcg cgagcgtctt gcggaactcg ttcagcaccg
    10201 cgttcctgtg ctcctcggta tccatgtcga tgaaatcgat gatgacgata ccgccgaggt
    10261 tcctcagccg cagctggcgc gcgatcacct gggccgcctc gaggttggtc ttgaagatgg
    10321 tgtcgtcgaa gctgcgcccg ctgacgaagc cgccggtgtt gacgtcgatg gtggtgagcg
    10381 cctcggtctg gtcgatgatg aggtagccgc cggacttcag ttccacgcgc cgcgcgagcg
    10441 ccttctcgat ctcctcctcg acgccgtaga ggtcgaacag cggccgctcg ccgctgtagt
    10501 gctcgatgcg ctcggcgacg ttgggcgtga agtcccggcc gaactcgagc atactccggc
    10561 aggtctcgcg cgagtccacc aggacgcgcg cggtgtcctc gtgcacgaag tcgcgcagca
    10621 cccgcaggct caggttgagg tcctggtaga ggggcgtcaa cggcgccgcg gcggcggcct
    10681 tgtcctggat gccgccccac agccgccgca ggtaatcgat atcggacttg agctcgcggt
    10741 cggaagcggt ttccgccacg gtgcggatga tgaagccgcc cttctcctcg ggcggcagca
    10801 gcagctgcag cttctcgcgc aggtgcgtcc gctcctcctc gtcctcgatg cgctgggaaa
    10861 tgccgatatg cgaatcctgc ggcaggtaga cgaggaagcg ccccgcgatc gagacctggg
    10921 tcgagagccg cgcccctttc gtgccgatcg ggtccttcac gacctgcacc atgaggttct
    10981 ggccctctcc gagcagcttt tcgatcggcg ccgcggaccc gccgttgcgg cgatgacccc
    11041 gcatgtcggc gacgtgcaga aaggccgcgc gctcgccgcc cacgtcgatg aacaccgatt
    11101 gcatgcccgg cagcacccgg atcacgcgcc ccatgtagat gttgccgacc agcccgcggg
    11161 aggaggcgcg ctcgacgtgc acctcctgcg tgacaccctg ctcgatcacg gccacccgcg
    11221 tctcttgcgg ggtgacgttg accaggattt cctcggtaac ggccattgtg tcggaggctc
    11281 tagaagaccg aaatgttgaa tttctgcagc aactccaccg tctccgcaag cgggagtccc
    11341 atgataccgg aatagctccc ggagatccgc gtcacaaacg accccgcccg cccctggatg
    11401 gcgtaggcgc cggccttgtc gagcggctcg ccactcgcgc agtagcgccg gatgtcggca
    11461 tccgggattt cgcggaacca cacgctggag accgagatct gcgtctccac ctgctccctc
    11521 agcgcgatcg ccaccgccga gagcacttgg tgctcgcggc cgcaaagcgc gcgcaggatg
    11581 cgcgccgcgt gctcggcgtc ctccggcttg ccgacgatct ggccgtcgaa cacgacggtg
    11641 gtgtcggcgg cgagcaccgg cttctgcggc agcccccggt acgcgatctg ccgcaccgcc
    11701 atctccacct tggcccgcgc gatgcgcagc acatacacgc cgggcgactc gtctgcaagc
    11761 ggcgtctcgt ccacgtccgc gccccgccgc aggtcctcgc gcagcagcag cagctcgaac
    11821 ggcacgccga tctgcttgag cagatcgcgc cgccgcgggc tacgggaagc aaggtagatg
    11881 cgttggtcgc tgagagccat tactatcgcg tggtgagtga gggatgaggg gtgaggagtg
    11941 aggagacgaa cccgtacaac ccgtctcaat gacgtgccga ctccttactc ctcactcctt
    12001 actcctcact cacggtggta gggatgattg tgcaatatcg aaacggcccg ataaagttgt
    12061 tcggccagaa ccacgcgcgc cagaccatga gggagcgtca gcggagacaa tgaccacacg
    12121 aaatccgagg attccttcac actctcggcc aagccgtccg cgccgcccac gatgaaggcg
    12181 acgtcccgtc ccgtctcccg ccagcgcgcg atgcgccgcg ccagatccat cgtgccgatg
    12241 agggtcccgc gctcatccag cgcgaccctg acacagcccc tgggcagcgc ggcgaggatg
    12301 cgctcccctt ccgtctcgag cgcgcgcttg accgggccgc cacgcgccgc cggcttgagc
    12361 gccacgagcc cgatgcgcgc ctcgcgcggc atgcgtcccg catactcggc gaaaccggcg
    12421 tccacccagg cgggcatgcg gtggccgacc gccaccacga ggaatttcat gaacgacgct
    12481 cgatccgagc ctagtgcaag cgcccaaccg ggcgtttgca cctcgaatgc gcccgcggag
    12541 ggcggagcag gatttccgtg cctatggcgt ggatgcgacc ccgcagcgga atgcagcgcc
    12601 gaattatccg cgtgctatcg tgcgcctccg cggtcgcatc cccataaacg acatggggcc
    12661 cctgctccgc gcttcggcgc accttcgcct ctcgggagaa gggcggggat gagggggtga
    12721 gcgtcgccat gcgtctcgcg acgctcagcg ggcgccggcc gcgcggcggc gccgggaccg
    12781 cggcggcgcc cacagctctt cgagattgta gtacttgcgg atggcgggct gcatgatgtg
    12841 aacgacgatc gcgcccaggt ccaccagtat ccactcgccc gcccgctcgc cctcgacgcc
    12901 gtggatgcgc gcgccgagcg ccttcatttt ttcctgtaga ttgttggaaa gcgccttgct
    12961 ctggcgggtc gagtcggcgc tcgcgatgat gatcttgtcg aacagcgagg tcattttcct
    13021 cgcgtccagc accacgatat cgcgcccctt gatgtcttca agcgccgcga ccgcggcctt
    13081 ggcaagcttg tccagtgtca tccactccct aggaataaag ccggttcgcc cgaatatagt
    13141 cgagaacctc ggcgggtagc aaataacggg ggctggcgcc tgaccgcacc atgtcgcgga
    13201 tcgccgtcgc cgagaggtcg agcgcggtga acgggacgac gacgatgccg ccggcgggct
    13261 gaagatgggt cgcgagcggc tgctgcatga ggcgcgccgt gtactcgcgc gcgagcggct
    13321 gcggcatgcg ctcggcccag cgctcgaccg ggaagccggg acggtgggcg acggcgacgt
    13381 gcgccagccc gaagatttcg tgccagcggt gccacgtcgc gaactcgaga aacgcgtccg
    13441 caccgaggag cagcacgagc ggacgcgagg cgccggcttc cgcgcgcagc tcggtcagcg
    13501 tgtcaaaggt aaagccggga cccgcgcggc gcacttcgcg ttcgtccacc ctgaagcgcg
    13561 gattgtccgc cgtcgcgagc cgcgtcatcg ccagacggtg ctcggccgca acctgcggcg
    13621 cgctgcgatg cggcgggatg ccgctgggga cgaagcgcac ctcgccgagc ctgagcgtct
    13681 cgcccagctc ttcgccgagg cgcaggtgcc cgtaatggat cgggtcgaag gtgccgccaa
    13741 ggacaccgat gggcgcggtt gacatggctc aaccgtcccg gtgagaagtg aggagtgagg
    13801 agtgaggagt aaaagccgcg aaaaccggct gatgtgtctt ccgcctcaca cctctcccct
    13861 cacacctcac gcataatgtc gtcaaaggac gagccgccgg atatcggaga gtacgctgct
    13921 caggaacgcg atgaagcgcg cgccctgcgc gccgtcgatc acccggtgat cgtaggagac
    13981 cgacagcggc agcatcagcc gcggcatgaa cgcgctaccg ttccacatcg ggcgcgtcac
    14041 cgacttgccc acgccgagga tcgcgacctc cggggcgttg atgatgggcg tgaagttgga
    14101 gccgccgagg ccgcccaggc tcgaaatgga gaagcagccg ccctgcaggt cggccggcga
    14161 aatcttgccc gcgcgcatgc gcgcgctcac ttcgcccagc tccttcgcca gctccatcag
    14221 ccctttcttg tccacgtcgc ggatcaccgg caccaccaag ccgttgggcg tgtccaccgc
    14281 gaccccgatg tggaagtact gcttgagcac gaggctctcg ccgtcggccg agagcgaggc
    14341 gttgaactcc gggtgctgct tgagcgccac caccacggcc ttgaggagga agcacaggat
    14401 ggtgaagcgg atgccctgct tcttcgcctc ctcgacctgg atcttgcgga attcctcgag
    14461 ctcggtgatg tcggcctcgt cgtgttgcgt gatgtgcggg atggtgaccc agttgcggtg
    14521 caggttggcg cccgagagct tcttgatgcg ggagagcggc tggttggtga cgggcccgaa
    14581 cttggcgaaa tccacctgcg gcagcggtgg cagactcagc gcgagcccgc cctcggccgc
    14641 gcgcgggcgc gagagctcgc ccttgacgta gctctggatg tcctccttga gcacacgctc
    14701 cttgggcccc gtgcccctca cgcgcgcgag gtccacgccc agctcgcgcg cgaagcgccg
    14761 tatcgagggg cttgcgtgcg cgcgcgcgaa acccgcctcg tccacccccc ccttcccaag
    14821 ggggggagat ggggggggtt ggggcgctgc agccggcgcc ggtgaaggag cgggcgccgc
    14881 gcgaggcgcg ggttgctgcg cgggaaccgg ggccggcttc tgcgccgggg attttgcgga
    14941 cgcaccgtcg cccggggatt cgagcgtgag gacgagcgag ccttcggaaa ccttgtcgcc
    15001 gatcttgacc ttgagctcct tcaccacgcc ggcggcgggc gaaggcacgt ccatcgtcgc
    15061 cttgtcggac tcgagcgtca ccagcgagtc ctcgggcttg accgcgtcgc ccggcttgac
    15121 gaagacctcg atcaccggca cgtccttgaa gccgccgatg tcggggattt tcacttcaat
    15181 cacattggcc atgcgcgctc aagatagaac gttgtctcag gtaaaaagtg aggcgttagg
    15241 cgttaggcgt taggcgttag gcgttaggcg ttaggcgtta ggcgttaggc gaaagaatct
    15301 gaaggccgat aggagttccg cctcactcct cacgccttac gcctcacact ttggcgggat
    15361 tcggtttctc cggatctaca ccgtacttct ttattgcttc aaccactttt ttctgcggca
    15421 gcgcctcctg gtccgcgagc gccttcagcg ccgccaccgc gacgtaacgc cggtccacct
    15481 cgaaaaagcc gcgcagcgcc cgccgcgtat cggaacgccc gaagccgtcg gtgccgagca
    15541 cgctatagtg cgccggctgc ggcaggtagg cgcggatctg atcggcgaac accttcatgt
    15601 aatcggtggc cgcgaccacc ggccccggat ggtctctcag gcactgctcg acataggagc
    15661 gcctgggctc ggcttccgga tggagcaggt tccagcgtgc ggtctcgacg ccgtcgcgcc
    15721 gcagctcgtt gaagctcgtg acgctccaga tgtcggccga gacgccgaaa tccttctcga
    15781 gcatctcggc ggcggcgatc acctcgcgca ggatcgtgcc gcagcccatg agctggaccc
    15841 tgagcttgtt cttcgccgcc ttacccctgg aaagcggata catgccgcgg aggatgccct
    15901 tctcaacccc ttccggcatc gcgggatgcg cgtagttctc gttcatcacc gtaacgtagt
    15961 aatagacgtc ctcctgctcc cggtacatgc ggcgcagccc gtcctggatg atcaccgcca
    16021 gctcataggc gaaagtcgga tcgtacgaca cgcagttcgg tatcgtggaa gccagcagat
    16081 ggctgtggcc gtcctcgtgc tgcaaccctt cgccgttcag cgtggtgcgg ccggaggtgc
    16141 cgccgagcag gaaaccgcgc gcgcgcatgt cgcccgccgc ccacgcgagg tcgcccacgc
    16201 gctggaagcc gaacatggag tagaagatgt agaacgggat catcggctgg ttgtgggtgc
    16261 tgtaggaggt cgcggccgcg atccaggagc acatcgcgcc cgcctcgcag atgccttcct
    16321 ggaggatctg gccgttcctg tcctccttgt agtacatcag ctggtcggcg tcctgcggcg
    16381 tgtagagctg gccgacgtgg gaatagatgc tgagctggcg aaacatgcct tccatgccga
    16441 aggtccgcga ctcatccggc acgatcggca ccacgagctt gccgatgccc ttgtcgcgga
    16501 tgatcgtggt cagtatccgc acgaacgcca tcgtggtcga gatctcgcgg ccatccgtcc
    16561 ccttcagctg ggcctcgaac gccgagagcg gcggcacttc gagcggctgc gacttacgcc
    16621 gccgggcggg cacgcttccg cccatcgcct ggatgcgttc cctgagatac ttcatctcgg
    16681 gactgtcttc cggcggcctg tagaacggca cttcctcgag cttgtcgtcg gggatcggca
    16741 cattgaagcg gtcgcggaac gccttgatcg aggaggttcc catcttcttc tgctgatggg
    16801 tgatgttctg gccttcgccc gcctcgccca tgccgtagcc cttgatggtc ttggcgagga
    16861 tcaccgtcgg ctggtccttg tgcttcatcg ccgcggcgta cgccgcgtag accttgtggg
    16921 gatcgtgccc tccgcggttc agccgccaga tgtcgtcgtc ggacatgtgc gcgaccatct
    16981 cgagcagctc cgggtacttg ccgaagaagt gcttgcggac gaacgcgccg tcttgcgatt
    17041 tgaaggcttg gtactcgccg tccacgcact cttccatgcg ccgcagcagg atgccggtct
    17101 tgtcgcgcat gaacagcgga tcccagtacg agccccagat caccttgatc acgttccagc
    17161 cggcgccgcg gaaggcggac tccagctcct ggatgatctt gccgttgccg cgcaccggcc
    17221 cgtcgagccg ctgcaggtta cagttgatga cgaagatcag gttgtcgagc tgctcgcgtc
    17281 ccgcgagcga gatcgcgccg agcgattccg gctcgtccat ctcgccgtcg ccgcagaagc
    17341 accatacctt gcgcccctgc ggctcgacga tgccgcggtc cttgaggtag cgcatgaagc
    17401 gcgcctggta gatcgccatg atggggccga gccccatgga caccgtcggg aactgccaga
    17461 agtccggcat caaccaggga tgcggatagg aggacaaccc cttgcctccg acttcctggc
    17521 ggaaatgctt caactgctcc tcggtgacgc gctcttcgag gtaggcgcgc gcgtagatgc
    17581 ccggcgcgga atggccctgg aagtacacga gatcgccgcc gaagtgatcg ttccaggcgc
    17641 ggaagaagtg attgaagccg acgtcgtaga gcgtggcggc ggaagcgaag ctcgcgatgt
    17701 ggccgcccag ttccgaggat tccttgttgg cgcgcagcac catcgccatc gcgttccagc
    17761 gcaccagcga gcggatcttg ttctccagct cggcgtcccc cggcgagcgc tcctcctgcg
    17821 acatcgggat cgtgttcatg tacgccgtgt tggcgttgta cgggatgtag gcgccgtggc
    17881 tccgcgcctt ctcgacgagc ctgtcgagca ggaagttggc gcgcgccggc ccttcacgct
    17941 ccagtaccga gtcgagcgcc tcgagccatt cccgtgtttc ctgcgggtcg aagtcgggtt
    18001 ggatgtctct cagatccata acggccaccg tttccaagtc caggagcttt tacagcgggc
    18061 aggcgacgct cgctgcgctt gcgccgggga aggcattata gcatcgggaa attgccgctt
    18121 ttgcgcggca atttaatgag atagctttac tgctgaccgg cgtgcgtccg ccgccttcaa
    18181 gcctggttga tcgactgatt taatggccct gaaacttggc agggcgcttt tccagaaagg
    18241 cgttcatgcc ttccatccgg tcctgcgtgg tgaaggcctg cacgaacaag tcggtctcca
    18301 ggtagcaggc ggagaagaga tcgagattgg ccccgcgctg gaccgcctgt ttggcgagcc
    18361 gcactgcgat cgggcctttg gacgcaatcg cgtgcgccat ttccagtccc tttgcccgca
    18421 gtccggacgc ggggcagaca tgattggcga gaccgatcgc gagcgcttcc tccgctttga
    18481 cctgccgcgc ggtcgtcagc agctcgagcg cgcgcgccgg cccgatccgg cgcggcaggc
    18541 gctgcgtgcc gccgaaaccg ggcgggatgc cgaggttgac ctccggctgg ccgaacacgg
    18601 cggcctcgga ggcgaggatc cagtcgcagg cgagcgcgag ctcgcagcct ccgcccaggg
    18661 cgtagccgtt caccagcgcg atcaccggca ccggcagcgc ttccagcgca tgcatcacgc
    18721 gcatgccctt ctcggaaaat tcgcgcgctt gctcgatcgt cgcgttctgc atctcggcga
    18781 tgtcagcgcc ggcgacgaac gctttttctc cggcaccagt gacgagcagc actcgtgctt
    18841 tgtcatccgc cgcgactttt ttgaccgcgg acgcaatctc gtccagcgtc gcggcgttga
    18901 gcgcgttcag cgccttcggg cggtttaccg tgaggaggta aatccccgcc tcgggctgtt
    18961 cgagcagaat gttctggaaa gccatcaaca cctcaaatca accaccaaga agaacacatc
    19021 aaaacaacaa tcgaaccgcc aaggctgcca agaacgccaa gatgaaaata ataaaaaatc
    19081 agaaactctt aaaggcgagt atatgccgga taagctacac tggcttttcc gcgcggtagt
    19141 tttcttgatg ctcgagattt tcgtttcttc ttggcgatcc tggcgtctcg actgttcaat
    19201 tattgtttgt ttttcttggc gttcttggcg gccttggcgg ttcattattt gattcgtgtc
    19261 tttgcggttc agcttttcac tttgcgggca tgcgcagcgc gccgtcgagc cggatcacct
    19321 ccccgttcag catcgggttc acgatgatgt gctcgaccag catcgcgtat tcctccggct
    19381 taccgagccg cgacgggaac ggcacctgcg ccgcgagcga ctgcttgagc tccggggcga
    19441 acgccgcgac catcggcgtc tcgaagatgc cgggcgcgat ggtcatcacg cggatgccga
    19501 agcgcgcgag ctcgcgtgcg gcgggtaacg tcaatcccgc gaccccggcc ttggacgcgc
    19561 tgtatgcagc ctgccctacc tgtccgtcat aggccgcgat ggaggcggtg ttgatgatga
    19621 tgccgcgctc gccttcggcg tccggctgat tttccgccat cgcctgcgcc gcgagccgca
    19681 gcacgttgaa ggtgccgatg agattgattt caatggcgcg gcggaaggtg tcgagactgt
    19741 gcggcccggt tttccccagc acgcgctcgc ccggagcaat tcccgcgcaa ctcaccgctc
    19801 cgtgcagcgc gccgaatttg ccgcgtgcag cggcgatcgc ctgcgtgacg cgcgcttcat
    19861 cggtaacgtc catcgggaca aacagcgcat gcgcgccgat ttccagcgca agctgttttc
    19921 cctggccagc gtcgagatca cagagcgcga cgttgccgcc ggcgtgggtg agccggcgcg
    19981 ccgtcgccgc gccgaggccg gaggcgccgc cggtgacgag gaaggtatgg cctgcgatct
    20041 gcatcgcaag tggtgattaa gtgattcagt gactgagtga ttgagaaacc cggccggttt
    20101 tcctcttaat cacttaatca cgtaatcact caatcaccgc ctactcgatc ttgatgccag
    20161 cctcgcgcgc gagcttcgcc tttttcacga actcggcccg gatgtactcc atgaatgctt
    20221 ccggcgaggc gctggcggtg agctcgacgc ccatctggat gaagcgctgg cgcggctcgg
    20281 gcgcggcgac ggccttagcg acgtccgcgt tcaggcgcgc gaggatttcg cggggagtcc
    20341 cggcgggagc gacgagaccg ttgaaagtga tgtcctcgaa gccccgcatg cccgattcgt
    20401 cgatggtcgg caggtcgggc aggagcggcg agcgcttgag gctcgtgacc gcgagccctc
    20461 tcagcttgcc ggccttgata tgaggcgtcg acgtgctcac ctggtcgaac atcatcgtga
    20521 cttgtccact gatgacgtcg acgatggcct gggcgttccc cttgtacggt acgtgcagca
    20581 ttttcacgcc ggcgtggcgg ttgaagagct cggccgccat gtggccggtg ccgccgacgc
    20641 ccgccgacgc ataggacatc tcgcccggtc gcgccttcgc cagcgcaacc agctgtttca
    20701 ccgaacgcac cggcagcgag gtattcacca ccagcacctg cggcaccagg caggtctgcg
    20761 agacgccgac aaagtctttg aggggatcgt agcccgggtt cgacatgatc gaagggacca
    20821 cggcaaacgt attcgccatc gcaagcaacg tatagccgtc gggcgctgct tttgccacca
    20881 gatgggtacc cagcaggccg ctcgagccgg ggcggttgtc ggggatcacc tgctgtccaa
    20941 gcgcctccga aagccgcggc gcaatgccgc gcacgacgag gtccacgttc ccaccgggcg
    21001 cgaacgggat gatgatgcgc accggcttgg ccggaaagct ctgggcctgc agggtggcgg
    21061 gcacgactgc aaccagaatg gctgcggtga gtaggcactt acttttcatt actcctccta
    21121 tacgaaagcg caccggcgcg gcggggtcag cccttgtccg cacgcatttt aacgtaggcc
    21181 cccggcgcgt cctcgatcgc cttgagcctg ccttcgccgg gaatgcatgc aggcaccttg
    21241 tcgcctccat gctgcgcgac ccagcccgcc cagtcgggcc accatgagcc cgggtgctgt
    21301 gacgcgccct tcaaccattg atccggctgc ccgggcagct tagggttggt ccagtacgag
    21361 tacttcttcg ccgccggtgg attgatgatg ccggcgatat gtccggagcc gccgagcacg
    21421 aagcgcaccg gagcggtgaa gagtttcgcc cccgcgtagg tcgagcgcca cggggcgatg
    21481 tggtcctcca gggttgaaac gaagtagagc ggtgccttta cccgggtcag gtcgatcggc
    21541 acgccgccga gcgtgatgcc cccaggcttt gcgagcaggt tcctgaggta caggttgcgc
    21601 aggtagtagc tgtgcatggt agccggcatg cgcgtcgaat ccgagttcca gtagaggaga
    21661 tcaaaaggga acggatcgcg cccgagaagg tagttgttga cgacgaacga ccagatgagg
    21721 tcgttcgccc gcagcaggtt gaacgtggtc gccatctcgg aaccttcgag atagccgcgc
    21781 tccagcatgc gcctctcgag cgcctcgacc tgcttctcgt cgatgaacac ctcgagctcg
    21841 cccgcctcct cgaaatcgat catggaggcg aagaacatcc ccgacacgat gcgctcgtcc
    21901 ttctttgccg cgagatagcc cagggtgcat gcgagcagcg tgccgcccag gcagtagccg
    21961 atcgcattca cttcgctctc gcccgtcgcc ttctcgatcg catccagcgc ggcgagcgtg
    22021 ccttcggcca ggtagtcgtc gaagcgcttg tgcgcgagcc tcttatcggg attgacccag
    22081 gagatggcga acacggtgtg cccctgctcg acggcccact tcacgaagga attggcttcg
    22141 cgcaggtcga ggatgtagta cttgtttatc cacggcggta tgatcagcag cggcctgcgg
    22201 tgaaccgggc cgctcgccgg gaagtactga atcagctgga tgaggtcgtt ctgaaacacg
    22261 accttgccgg gtgtcgtcgc gatgtttacg ccgagcttga acgcattctc gtcggtcatg
    22321 cgcagctgga tctgctctcc gccgccgcgc gagaggtcct ccagaaggtt gttcagtccc
    22381 ttcaccagat tgtgcccccc gctcgccacg gtctcgcgca acacctcggg gttggtgtgg
    22441 aggaaattgc tcggcgagag cgcatcgatg tattgccgcg tgtagaactc caccttcctt
    22501 gcggtctgct cgtccagccc ctcgaccttg cccagcgtct ggtgcaggtg ccgggcggcg
    22561 atcaggtagg actgcttgat gtagtcgtag agaaaattct gctgccaatc ctcgtgctgg
    22621 aagcgacggt cgccctggcg cggctcggcg accggcttcg cgtcctgccc catcagcttc
    22681 agcatcgagc tctgccacag cgacagagtg tcctgcaaat atctcagttg cgcctcggcg
    22741 agcttgaacg gatccgatag cagcctggcc cacgcctgga gaaacgcttg cgcgatgccg
    22801 agctcgtcgc ccattccgcg catcccgccg ttctggctgc gttccagaaa ttgcgttatg
    22861 agtcggccgc tcttctgtgc gatatcagcg tagagccggg ccagctcaga gggatcgtag
    22921 cctctctgat cctgtcgtcg ttcctcggac attttgtttt gccactttct tgacgtttac
    22981 gtaaacgtgc tataggctgg atattgctta tcttagccgg cattgctgca tagcacaatg
    23041 agtacgactt acacaatttc tgacctcgcc agggaattcg atgtcacgac tcgcaccatc
    23101 cgcttttacg aggaccaggg gctgctgagc cccaagcgcg agggcaccag ccggattttc
    23161 agcaaccgcg atcgggtgcg gctgaaactg gcgctgcggg gcaaacgcct cggtttctct
    23221 ctcgcggaga tccgcgagct attcgagttg tatgacctct cgcgcgacga gcgcgagcag
    23281 ctcgaagaat ttctggggaa actggagcgg cggcgggcac acctcgagca gcaacgggaa
    23341 gacatcgagg ttatgctgaa cgagatcaac ttctttgcca accaatgcaa gcggcttttg
    23401 aaagataaac cggctagtga ggataccaag ccgaccactt gagtcggatt tttgcagtgc
    23461 aagctatatt tacgttaacg taaacgttat atgaaaaacc gcctgaaaac gataggttat
    23521 cttattttcg caagcgttcc agggtgggaa ccttcttcct ttgaaacgcg agcagttaga
    23581 cctgcgttca ggcatccagc tctaacggtg atggagtggc gcaagagcat ccattcatgc
    23641 tcgcgcggtt attctggagc ctggcgcccc gagtgacctg gaggatcgcg tgttgcaagg
    23701 tctcaatttc gagctgggcg aaactgtcga catcctgcgc gagaccgtct cctcgttcgc
    23761 ggcgcgggaa atcgcgccgc gcgccgccga gaccgaccga tccaatgaat tcccgcgcga
    23821 tctctggccc aagctcggca agctcgggct gcttggcatc acagtcgagg aagaatacgg
    23881 cggcgccggc atgggttatc tcgcgcatgt cgtcgcgatg gaggaaatca gccgcggttc
    23941 agcctcggtc ggtctgtcct acggcgcgca ctccaatctg tgcgtcaacc agatccgccg
    24001 caacggcagc gcggagcaga aacggcgcta cctgccgaag ctggtcgcgg gggaacacgt
    24061 cggcgcgctg gcaatgagcg agagcggcgc cggttccgac gtcgtgggca tgcggctgcg
    24121 cgcgcaaaag cgtggcgacc gttacgtgct caacggcacc aagatgtgga tcaccaacgg
    24181 cccggacgcc gacgtcctgg tcgtttacgc caagaccgat cccgccgccg gtgcgcgcgg
    24241 catcaccgcg ttcgtcatcg aaaaaagctt caagggcttt tcgaccgcgc aaaagctcga
    24301 caagctcggc atgcgcggct ccaacacctg cgaactcgta ttcagcgatt gcgaggtacc
    24361 ggaagagaac gtcctgggcg cggccggcaa gggcgtcgag gtgctgatga gcggtctcga
    24421 ttacgagcgc gtggtgctcg ccgccgggcc gctcggcatc atgcaagcct gcttcgacgt
    24481 cgtgcttccg tacctgcacg agcgcaggca gtttgaccag ccgatcggtg aattccagtt
    24541 gatgcagggc aagctcgccg acatgtacgt gaccctcaac gcctgtcgcg cgtacgttta
    24601 tgcggtgggc cgcgcgtgcg atcggggcga ggtcacgcgc aaggacgcgg ccggcgcgat
    24661 cctgttcgcc tccgaaaaag cgacgtggat gacgcttgaa gccatccagt gcctcggcgg
    24721 catgggctac attaacgata cgcccaccgg ccgtctgctg cgcgacgcca agctttatga
    24781 aattggggct ggaacgtctg aaataaggcg atggctgatc ggaagagagt tgtacgacga
    24841 aacgcgataa cgtatgccgt gtcccaattc ggtgaaggct aacatgagcg cagagaatgt
    24901 taagcgcgcg gcgtcatccg cttgcttcag caagcacccg gacgcgcgcg gccgtaacca
    24961 aatcggcgcg ggcacttccg aaattaggcg ttggttgata ggtcgtgaac tctacgatga
    25021 gacccgctaa tacgtcattg ccgccgattc ggcgaaggct aacatgagct tgaatcggac
    25081 gcgcagcgcc ggttcaagtc cgccggttgc aggcggacag ggggctgccg ctaaaccggc
    25141 ggatagcaag tgacaatcat ccggccacag ccgatatgac gcaggcgttt ccgagatcag
    25201 gtggtggttc atcggccgga aactgttaga ggaaacgaag tagggatcag cgtgctccgg
    25261 tgaaggagcc tgatgctacg agactgatgg agcgagaccg aagcagcgag actggaaaaa
    25321 cgggcttagc ctgcttatca ccctttactc atcacatatc attcctttgg ccatgatcga
    25381 tccacgatct actacccgcg aatcgtgtat tttctgctac gaagtgacca ctaactaacg
    25441 ctgaaatcac tcgataaatg gccaaattgc atacgcccct ctgcgaccgg ttgggtatcc
    25501 gcctgccgat catccaggcg cccatggcgg gcggggcatc ctctaccgaa ttggtggccg
    25561 cagtgagccg tgccggtgcg ctcggttcct tcggcttcgc ctatacccag cccgagagca
    25621 tgcagcggga cgcggaggca gtgcgagcac acactcacct gccgttcaac ctcaatcttt
    25681 tcgcctcgca gcagcccggc gccattgatc ccgcagcaca gcgcggcgcc atcgatgccg
    25741 tcgccggcta ctacgccgag ctgggtctgc ccccgcccga gccggcgcgc ccgccctatg
    25801 cgccggacct cgaggcgcag tttgccgcgg cggaggaaat acgcccgccg gtgctcacct
    25861 tccacctcgg cgtcatgcag cacga
//
LOCUS       DBNGWZ010000669         3022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1127976, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000669 DBNGWZ010000000
VERSION     DBNGWZ010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1127976"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="AC16CX_20885"
     CDS             complement(<1..875)
                     /locus_tag="AC16CX_20885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR3014998.1"
                     /translation="MPVKTHSFLAAAVMLGSVALVTEAAEKSYPERPVRVLVGLAPGG
                     GTDTISRVMTQKLSEMLGQPFIVDNRPSSGGNVAGELAARAAPDGYTLITVTPTHVVN
                     PSLFRDVRYDAVRDFAPIVLMVYTNYVLSVGALVPVTSVKELIALAKSRKQRLTYAST
                     GIGSANHLSAELFKSMAGIDMVHVPYKGGAPALSALLSGEVQVMFSSSGGLFPHVKAG
                     RVRALAVTGAKRSLSAPDIPTIAESGVPGYDVTGWYGLATTAGTPKVVIERLNATVNR
                     LLPELRERYANLGTEV"
     gene            complement(1017..2381)
                     /locus_tag="AC16CX_20890"
     CDS             complement(1017..2381)
                     /locus_tag="AC16CX_20890"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3014999.1"
                     /translation="MTTSTTTITPDLVRYMAEARTSPIPSLVVAKAKHHILDTLAAMV
                     SGTTIKPGLFAIRYIGQQGGNPEAQVVGSQIVTSAINAAMANGMLAHSDETDDSNGSA
                     GIHPGCAVIPAALAMGERENASGEDFLRAVILGYDIGSRTTKALGRDALRARNHLPFS
                     IGGTLGASAAAGSVAHLSEEQFRHLLSCAAQQASGVMTYARDTEHVEKAFIFGGMPAR
                     NGVTAATLVQIGYTGVYDVFDGHGNFLAAYAESPKPQELTAELGSRYEVMLTNIKKYC
                     VGFPIQSALEGLINLMAEHQLRADQVERITARLPESGAHTVNDREMPDINLQYVFAVT
                     LLDGRLTFEAAHSFERMKDPKVLELRKKVTLIGDPELTRARPEGQSIVQVITRDGRQL
                     EKRTTTFKGRSENPLTTGEVEEKARELLEPVLGQPRARRLMDAVDKIESLASVRDLRP
                     LLRA"
     gene            complement(2378..2593)
                     /locus_tag="AC16CX_20895"
     CDS             complement(2378..2593)
                     /locus_tag="AC16CX_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015000.1"
                     /translation="MQATMMRFPLTLTHIFERAGRLFPESEIVSRMPDKSLHRYQYAN
                     FYRRIEFALAPQERLSKNQPTPGAEKR"
     gene            complement(2742..>3022)
                     /locus_tag="AC16CX_20900"
     CDS             complement(2742..>3022)
                     /locus_tag="AC16CX_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015001.1"
                     /translation="VKERFMKKASRKPVDELRPEYKRSDFGTLVRGKYAARVSESSNV
                     VVLQPQVARAFPNDRAVNEALLKLLRGRKSSASAGERSAPTRQKRRAG"
BASE COUNT          563 a          944 c          905 g          610 t
ORIGIN      
        1 acctccgtgc cgagattggc atagcgttcc cgcagttcgg gcagcaggcg gttcacggtg
       61 gcgttgagac gctcgatcac cactttcggc gtcccggccg tggtcgccaa gccataccag
      121 ccggtcacgt cgtaacccgg cacgcctgac tccgcgatcg tcggaatgtc cggcgctgac
      181 agtgaccgtt ttgctcccgt cacggcaagg gccctgactc tgcccgcctt cacatgaggg
      241 aagagtccgc cactgctgct gaacatcacc tggacctcgc ccgaaagcag cgcactcagg
      301 gccggagccc cacctttgta aggcacgtgg accatgtcga ttcccgccat ggacttgaac
      361 aactccgccg aaaggtgatt cgcgctgccg attccggtcg atgcataagt aagtcgctgc
      421 ttgcggcttt tggccagggc aataagctct ttgaccgacg tgaccggtac caaggcgccc
      481 acggacagaa catagttggt gtacaccatc agcacgatcg gcgcgaaatc cctcaccgcg
      541 tcgtagcgga catcgcgaaa caggctcggg ttcaccacat gcgtcggcgt aactgtgatc
      601 aacgtatagc catccggcgc ggcgcgcgct gccagctcac cggctacgtt gcctccggag
      661 ctcggtcggt tgtcgacaat gaacggttgc cccagcatct cggaaagttt ctgcgtcatc
      721 acccgcgaga tggtatccgt ccctccgcca ggagccagcc cgaccagcac ccttaccggc
      781 ctctccggat aagacttctc cgcagcttcg gtcaccagcg cgacgctgcc gagcatcacg
      841 gccgccgcga gaaaagaatg agttttcacc ggcattctcc tggaactttg gaaattaatc
      901 ggatacttca accaccgagc gcgccgatca atcgcttccg atgttgagct gagggtgatc
      961 ttcctccttc atggcgccgg cgccgacgag cggcgcccat gggcattcga tatcgcctac
     1021 gccctcagca atgggcgcag gtcccgcacg ctggctaggc tttcgatctt atcaacagcg
     1081 tccatcaacc gccgggcgcg tggttgcccc aggaccggct ccagcagttc ccgggctttc
     1141 tcctcgactt cgccagtggt cagagggttc tccgaccggc ccttaaaggt ggtcgtccgc
     1201 ttttccaact ggcgaccgtc tcgagtgatt acctggacga tggactggcc ttccggtcgg
     1261 gcccgggtca actccgggtc gccgatgagc gtgaccttct tcctgagctc caggaccttg
     1321 ggatccttca tccgctcaaa ggaatgggcc gcctcgaagg taagcctgcc gtccagcagg
     1381 gtaaccgcga acacgtattg caggttgatg tcgggcattt cccggtcatt tacggtgtga
     1441 gcgccgcttt cgggaagacg ggcggtgata cgctcgacct ggtctgccct gagctggtgc
     1501 tccgccatca ggttgatcag cccttccagg gccgattgga tggggaagcc gacgcagtat
     1561 ttcttgatgt tggtcagcat gacttcatag cggctaccca actccgccgt cagttcctgc
     1621 ggcttggggg actcggcata ggcggccagg aaattgccgt gcccgtcaaa gacatcgtaa
     1681 acgccggtgt agccgatctg taccagagtg gcggcagtca cgccattccg ggcgggcatt
     1741 ccgccaaaga tgaaggcttt ttcgacatgc tccgtatccc gcgcgtaggt catcacgccg
     1801 gacgcctgct gggcagcgca cgagagcaga tgccggaact gctcttcgct gaggtgagcc
     1861 acggagcccg ccgccgcgct tgcgcccaag gtgccgccga tgctgaacgg cagatggttc
     1921 ctcgcgcgca gcgcgtcccg acccagggcc ttggtcgtgc gggagccgat gtcgtaaccc
     1981 agaatcaccg cccgcaggaa gtcctccccg ctcgcgtttt cccgctcccc catggccagg
     2041 gcggcgggga tcacggcgca gcccggatgg atcccggcgg aaccattcga gtcgtcggtc
     2101 tcgtcagaat gggccagcat gccgttggcc atggcggcgt tgatggcgga agtgacaatc
     2161 tgactgccca ccacctgggc ttccgggttc cctccttgct gcccgatgta gcgtatcgca
     2221 aagaggccgg gtttgatggt agtcccggag accatcgccg caagagtgtc cagtatgtgg
     2281 tgtttggctt tcgccacaac cagggacgga atgggcgatg ttctcgcttc tgccatgtag
     2341 cggaccaggt cgggagtgat ggtagtggtg ctggttgtca tcgcttctct gctccgggtg
     2401 tgggctggtt cttgcttaat cgctcctgag gagcgagagc aaattcaatt cggcggtaga
     2461 aattggcgta ttggtaccga tgcagcgact tgtccggcat gcgcgacacg atctcggact
     2521 cggggaagag cctgcccgca cgctcgaaga tgtgcgtgag agtgagggga aaccgcatca
     2581 tcgtggcttg catgacggta gtttacctct caccgcggag gacgccgagg acgcggaaga
     2641 gaagcaagaa acagagagga tttacaggat taacaaaggc cgaaccgcgg cctttgaatc
     2701 accgataacc cagcgggatc gaaccagctt ccaatacctt cttagccggc tcgtcgcttc
     2761 tggcgcgttg gcgccgaccg ttcgcctgcg gaagcggaac tttttcggcc gcgcagcaac
     2821 ttaaggagag cctcgttcac ggcgcgatcg ttcggaaagg cccgggcgac ctgaggttgc
     2881 agcaccacaa catttgatga ttcggatacc cgtgccgcgt actttccccg cacaagagta
     2941 ccgaagtcag agcgcttata ctccggccgg agttcgtcca caggtttccg actagccttc
     3001 ttcataaatc gttctttcac cc
//
LOCUS       DBNGWZ010000670         4027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3269953, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000670 DBNGWZ010000000
VERSION     DBNGWZ010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3269953"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16CX_20905"
     CDS             complement(<1..222)
                     /locus_tag="AC16CX_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3015002.1"
                     /translation="MRTWFWHHLDALRTTLARLLRTPVATLLNVSVIGVALALPVWLY
                     VAVVNLEGLARSLGSDPQLSVFLALDAGPA"
     gene            complement(656..1147)
                     /gene="ahbB"
                     /locus_tag="AC16CX_20910"
                     /pseudo
     CDS             complement(656..1147)
                     /gene="ahbB"
                     /locus_tag="AC16CX_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858439.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
     gene            complement(1144..1587)
                     /locus_tag="AC16CX_20915"
     CDS             complement(1144..1587)
                     /locus_tag="AC16CX_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HIR3015003.1"
                     /translation="MDELDRAIVNALQGGFPVSERPYRDAAAQLGIGEDELIERLRKL
                     LEQQVLTRFGPLWQIERIGGAYGLAAMKVPPAELDAVAGKVNALTEVAHNYERDHPFN
                     LWFVLATATPDAIVAAAARIERETGHRVYLMPKVEEYFVRLELSA"
     gene            complement(1690..2679)
                     /gene="ahbB"
                     /locus_tag="AC16CX_20920"
     CDS             complement(1690..2679)
                     /gene="ahbB"
                     /locus_tag="AC16CX_20920"
                     /EC_number="4.1.1.111"
                     /inference="COORDINATES: protein motif:HMM:NF040707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme decarboxylase subunit beta"
                     /protein_id="HIR3015004.1"
                     /translation="MDRLDLRLIDGFQRGFPLASRPFRAIAERLGSSEEEIIARLKAL
                     SAGCAVSRVGAVFAPGRVGAGTLAALSVPSARLAEVAALVSAQHGVNHNYEREHDWNL
                     WFVVTAPDEARVRQTLDVIESRAGCGKLLRLPMLEGYHIDLGFPLGEAAPRAAVPTGR
                     GQAQLGAKGRALASALQDGLDLVPAPYAALAARAGITESEVIAALGEWLESGIVKRLG
                     LIVRHHELGYRANAMVVWDVPDDRVSECGRRIAAVPYVTLCYRRARCLPQWRYNLYCM
                     IHGQSRTRVLAQIAKLDVRCHLVEYPYEVLFSRRRFKQAAARYIEAENVEVAA"
     gene            complement(2695..3867)
                     /locus_tag="AC16CX_20925"
     CDS             complement(2695..3867)
                     /locus_tag="AC16CX_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HIR3015005.1"
                     /translation="MRRRLAALALVAPLVAGCAAPAPRGTGDLGVVVERAAGRVQVVD
                     TTRRAAIASVSGLGDLSHASVVYSRDGRYAFVFGRDGGLTKIDLLRARVERRVVQSGN
                     SIGGAISQDGSLIAAANYTPGGVKVFSADTLELLADIPTVYDGGQRAKVVGIEDAPGN
                     RFVFSLFEAGEIWLADLTEPRHPKIQKFRGIGREPYDALVTPDGRYYLAGLFGEDGLA
                     LLDLWHPERGVKRVLPGYGRGEEKLPVYKMPHLEGWAIAGRFAFVPAVGRHEVLVVDT
                     EDWREAGRIAVAGQPVFVIARPDGRQVWVNFALPHNDRVQVIDTESRRVIETLRPGRG
                     VLHMEFTPRGEMAWVSSRDDDRVVVYDTASLRVVTEIPSEKPSGIFFTSRAFRTGF"
BASE COUNT          611 a         1458 c         1373 g          585 t
ORIGIN      
        1 ggcgggtccg gcgtccagcg ccaggaatac cgagagttgg ggatcggagc cgagcgagcg
       61 cgcgagcccc tccaggttga cgaccgcgac atagagccag accggcagcg cgagggcgac
      121 gccgatcacg ctcacgttga gcagcgtggc aactggcgtg cgcaggagcc gcgcaagcgt
      181 cgtgcgcagc gcatccaagt gatgccagaa ccaggttctc atgccgccgc ctcttctagg
      241 ggcatcatga cgcgaacgcc tcgcgtcggc ggcatgcgtc atggcccgcg gatggcggag
      301 cagtgcctgc gcccgtgggc gtaggtgcga cccgggagcg ggccaagcgc gccggttcca
      361 atccgcgtgc ctctgcgcgc ctctgcggtc gcatccccgg aaaacggggc ccctgctccg
      421 cgctccggcg cgccggtcgc atccccgtga acggggcgcg tcgacgggcg gaagaggggc
      481 ccctctgggg atctgacccc ggagacgcgc atgggatcgg cattggaacg acgcgccctt
      541 gatcggctcc acggtcgcat cccgcaagcg ggcccctgct ccacgttgcg ccgatccctc
      601 ttccgcccct cgacgcgctc ccgtcactcg tcactcgaca ctcgtcactc gtattcagcc
      661 gcacgccggt cttcttgagg atgcgcgtgc tgtagagcac cgcgtggccc cggtcggcat
      721 cgccgacaat cgtcgcgatc tcgcgcactt tcgactcgac ttcgccgcgg gtcctgccat
      781 gcaccatcgc gaacaggttg taaggccact cgccgggatg ccggacccgc tggtagcagt
      841 gactgacaaa cgacagcgcc ccgatgcggc gcccgagttc gccagcgcgc tcgtccggaa
      901 cgtcccacac gctcatgccg ttcgcccgga agccgagcgc gtagtggttg ggcacggcgc
      961 cgatgcggcg gatcgccccg gcctcgagca tgcgccggat gcggaccatc acctccccgg
     1021 cgggcacgcc gatgcgctcg gcaaccgcgt ggtaggggcg cggcgtgagc ggcagcccgg
     1081 cctgcgtcgc ggcgacgatg cggcggtcga tttcgtcggg aaggaaggga gcgcgcggcg
     1141 cgttcatgcg gaaagctcga gcctgacgaa atactcttcc accttgggca tgagatagac
     1201 gcggtgcccg gtctcgcgct cgatgcgggc ggcggcggct acgatcgcat ccggcgtcgc
     1261 ggtggccagg acgaaccaca ggttgaacgg gtggtcgcgc tcgtagttgt gcgccacctc
     1321 ggtgagcgcg ttgactttcc cggccacggc gtccagctcg gccggcggca ctttcatggc
     1381 ggcgaggcca taagccccgc cgatgcgctc gatctgccac aggggcccga agcgcgtgag
     1441 cacctgctgc tcgagcagtt tccgcaggcg ctcgatcagc tcgtcctcgc cgatgccgag
     1501 ctgcgccgcg gcgtcacggt atggccgctc gctgaccggg aagccgccct gaagcgcatt
     1561 gacgatcgcg cggtcgagct catccatggg cggctgcctc cgaacagata tctggatcac
     1621 attcacatcg cgtcggcagc cctgcgtcct tcgcgggtcg catccccgga ggggcccctg
     1681 ctccgccgct catgccgcta cctccacgtt ttccgcctcg atgtagcggg ccgccgcctg
     1741 cttgaagcgg cggcggctga acagcacctc gtaggggtac tccacgagat ggcagcgtac
     1801 gtcgagtttc gcgatctgcg cgagcacgcg cgtgcggctt tgcccatgga tcatgcaata
     1861 gaggttgtag cgccattgcg gcaggcagcg tgcgcgccgg tagcacagcg tcacgtaggg
     1921 aaccgccgcg atgcgccggc cgcactccga taccctgtcg tcgggaacgt cccacacgac
     1981 catcgcgttg gcccggtagc cgagttcgtg gtgccgcacg atgagcccga ggcgtttgac
     2041 gatccccgac tcgagccact cgcccagtgc ggcgatgacc tcgctttcgg tgatgccggc
     2101 acgggcggca agcgcggcgt acggcgcggg cacgagatcg aggccatcct gcagcgccga
     2161 tgcaagggcg cgccccttgg cgccgagctg cgcctgcccg cgtccggtcg gcaccgcggc
     2221 ccgcggcgcg gcctcgccga gcgggaaacc gaggtcgatg tggtagccct cgagcatcgg
     2281 gagacggagc agcttgccgc agccggcgcg cgactcgatg acatccagcg tctgccgcac
     2341 gcgggcctca tccggggccg tgacgacgaa ccacaggttc cagtcgtgct cgcgctcgta
     2401 attgtgattg accccgtgct gggcactcac cagcgccgcg acttcggcaa gccgcgcgga
     2461 gggcaccgac agcgccgcca gcgttcccgc tccgacccgc cccggcgcga acaccgcgcc
     2521 gacgcggctc accgcgcagc cggccgaaag cgccttgaga cgcgcgatga tttcctcctc
     2581 gctgctgccg agtcgctcgg cgatggcgcg aaacggccgc gacgcaagcg gaaagccgcg
     2641 ctggaagccg tcgatcaatc gcaggtcgag tcggtccacg ctgcagcccg atggtcagaa
     2701 gccggtgcgg aaggcgcgcg aggtgaagaa gatgccgctc ggcttctcgg acgggatctc
     2761 cgtcaccacc cgcaggctcg ccgtgtcgta gaccacgacg cggtcgtcgt cgcgtgacga
     2821 gacccacgcc atttcgccgc gcggcgtgaa ctccatgtgc agcacccccc gccccggccg
     2881 cagcgtttcg atgacgcgcc gcgactcggt gtcgatcacc tgcacccggt cgttgtgcgg
     2941 cagcgcgaag ttcacccaca cctggcggcc gtccgggcgc gcgatgacga acaccggttg
     3001 ccccgcgacc gcgatgcgcc ccgcttcgcg ccagtcttcc gtgtccacca ccagcacctc
     3061 gtggcgtccg accgccggca cgaacgcgaa gcggcccgcg atcgcccagc cctcgaggtg
     3121 cggcattttg tataccggca gcttctcttc gccccgtccg tagcccggga gaacgcgctt
     3181 cacgccccgc tcggggtgcc acaggtcgag cagcgccagc ccgtcctcgc cgaacaggcc
     3241 ggcgaggtag tagcgcccgt cgggcgtcac cagcgcgtca tagggctcgc gcccgatgcc
     3301 gcgaaacttc tggatcttcg ggtggcgcgg ctcggtgagg tcggcgagcc agatctcgcc
     3361 cgcctcgaac aggctgaaca cgaaacggtt gcccggcgcg tcctcgatgc ccaccacttt
     3421 cgcgcgctgg ccgccgtcgt aaacggtggg aatgtccgcc agcagctcga gcgtatccgc
     3481 cgagaacacc ttgaccccgc ccggcgtgta attggcggcc gcgatgaggc tcccgtcctg
     3541 cgagattgcg cctccgatgc tgttgcccga ctgcaccacc cggcgctcga cgcgcgcgcg
     3601 cagcaggtcg atcttggtga gccccccgtc gcgcccgaac acgaacgcgt agcgcccgtc
     3661 gcgcgaatag acgaccgagg cgtgcgacag atcgccgagg ccgctcaccg atgcgatggc
     3721 ggcgcgccgc gtggtgtcca cgacttgcac ccgcccggcc gcccgctcca ccaccacgcc
     3781 gaggtcgccg gtgccgcgcg gtgcgggcgc ggcacacccc gcaacaagcg gcgcgaccag
     3841 cgcgagggcg gcgagcctac ggcgcatcgg gaaagccggc cagaagctgc cgcgcgatcc
     3901 acgcgacgtc ggcgtcggac aggatcggac gccagccggg catcgcggtg ccgtgtcggc
     3961 cgttgaggat gaggattgca agcgcgtccg gcgatatcgc gccgagcgcg gccggcgtca
     4021 atgccgg
//
LOCUS       DBNGWZ010000671         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_873603, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000671 DBNGWZ010000000
VERSION     DBNGWZ010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_873603"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="AC16CX_20930"
     CDS             complement(<1..436)
                     /locus_tag="AC16CX_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3015006.1"
                     /translation="MTITIHVNGKRHAVAAEPETPLLYILRNDLKLKGARFGCGLGQC
                     GACTVLVEGKPVQSCDFPLSAAAGKAVTTVEGLAGDGKLHPLQQAFITEQAAQCGYCV
                     TGIIIAAKALLDANPRPSETDIRAALKGNLCRCGTHQRILRAI"
     gene            616..825
                     /locus_tag="AC16CX_20935"
     CDS             616..825
                     /locus_tag="AC16CX_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR3015007.1"
                     /translation="MIHATDVLALKQKINQLQTEHRDLDDVISRLSQSPAQDQLQLQR
                     LKKRKLFLKDQIAMLERQLEPDIPA"
     gene            complement(996..2156)
                     /locus_tag="AC16CX_20940"
     CDS             complement(996..2156)
                     /locus_tag="AC16CX_20940"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3015008.1"
                     /translation="MIIDCDTHYLPPDAYDHMGPEWEDRRPRFRWDDKGLLTGVDFPG
                     APPRVPGATPLEPPGTGARYRGMYDMEERLRDYQKLGIDRQFLFPQLTATVFSYLVEP
                     RLANAMAHSYNLSVLRLVQRHPEQLIGGALVALQDVEGAIRELEWARENGFRAVILDK
                     VFPVHEHCFSDPYGSHRELWPFFRRAEALGMPLFLHNIQHGHRITNLLNYQFNGLDVL
                     APQEGQMSLVSLITSGLLDDFPGLDFVFTESGVSFLKPLAAHLDAAHGDALVDYESED
                     AAARFNYRKLTGGKRIVSEEDYKAKNRRPPSYYFRNNLYFTIETEEAELGEAVGLFGA
                     TRFLFATDYPHDDPGGRMKFRDVELLEQRSDISAQDKELILSGNALRLLGHA"
     gene            complement(2153..2698)
                     /locus_tag="AC16CX_20945"
     CDS             complement(2153..2698)
                     /locus_tag="AC16CX_20945"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR3015009.1"
                     /translation="MRTIAGLPLMPRLGYALGFLTCAGLIGFALYLQYYEYQDPCPLC
                     LLQRLVYMALMAVFLVAALHGPGRLGAIVYGGLLFTTAAIGAAIAARHVWLQHLPRHQ
                     VPECGPGLEYMLRKFPLWQAIEKVLGGSGECAETGWTFLGLSIAGWSLAWFVLLGIFS
                     VYLALIGMRRPSGADRARAGS"
     gene            2777..>3103
                     /locus_tag="AC16CX_20950"
     CDS             2777..>3103
                     /locus_tag="AC16CX_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015010.1"
                     /translation="MNTDISNAEEVISKGSRSRLSVPICRQFALLYLEIQDLAHESKK
                     RFELRIIRDKRRCMERQIIGLFSIDLRSPAFIGGSWVLDIIETGSSCAGRKRAVHSTH
                     DQSGARH"
BASE COUNT          618 a          985 c          925 g          575 t
ORIGIN      
        1 ggatggcgcg caggatcctc tggtgcgtgc cacagcggca caagttgcct ttcagcgcgg
       61 cgcggatgtc cgtctccgac gggcgcggat tggcgtcgag cagcgccttg gcggctatga
      121 tgatgccggt gacgcagtag ccgcattgcg ccgcctgctc ggtgatgaat gcctgctgca
      181 acggatgcag cttgccgtcg ccggcgagcc cttcgacggt ggtgacggcc ttgcccgccg
      241 ccgcggacag cggaaaatcg caggactgca ccggtttgcc ctccaccagc acggtgcacg
      301 ctccgcactg gcccagcccg cagccgaagc gggcgccctt caacttgaga tcgttgcgca
      361 ggatgtagag cagcggcgtt tcaggctccg ccgcgacagc atgtctcttg ccgttgacat
      421 ggatcgttat ggtcacaatc acttccttta cccggcgcgc acgccaggct tgtcaagaaa
      481 aaacagtggc ttattccctc gccatcgaaa cccggctgat cccggtcgga cgcgccggct
      541 agcgggctcg ttttttgaaa ttattgggtc tatactggaa aaacgcgtgt aacgttggta
      601 aggaggatgc cgcccatgat acatgccacc gacgtcctgg ctctcaagca gaagatcaac
      661 cagctgcaaa cagagcaccg agacctcgat gacgtcattt cccgtttgtc gcaaagcccc
      721 gcccaagatc agttgcagtt gcaacgcctc aagaaacgca agctcttcct gaaggaccag
      781 atcgccatgc tcgagcgcca gctcgagccc gacatacccg cctagccgcg ggcaaattcc
      841 aagctttaaa catcaagctc caagtttcaa gatgcaacgc tctgacgttg cttcttgttc
      901 cttgtgcctt gtcatttggt gcttgtcgct tgtttggtgc ttgcgaattg gtgcttgttg
      961 tttgatatcg ggtacttgcg cacccccggt gcgtgtcagg cgtgcccgag caagcgcagc
     1021 gcgttcccgc tgagaatcag ctccttgtcc tgcgcgctga tgtcggagcg ctgctccagc
     1081 agctccacgt cccggaattt catgcgcccg cccgggtcgt cgtgcgggta atcggtcgcg
     1141 aacaggaacc gcgtcgcgcc gaacaggccg accgcctccc ccagctccgc ttcctcggtc
     1201 tcgatcgtga aatagaggtt gttccggaaa taataagaag gcggccgccg gttcttggcc
     1261 ttgtaatcct cttccgaaac gatgcgcttg ccgcccgtaa gcttccggta gttgaagcgc
     1321 gccgcggcat cctccgattc gtaatccacc agcgcgtcgc catgcgcggc gtccagatgc
     1381 gcggcgagcg gcttgagaaa cgacacgccc gactcggtga acacgaagtc gagccccggg
     1441 aagtcgtcga gcagcccgct ggtgatcagc gacaccaggc tcatctggcc ttcctgcggc
     1501 gccagcacgt cgagcccgtt gaactggtag ttgagcaggt tggtgatgcg gtggccgtgc
     1561 tggatattgt gcaggaacag cggcatcccg agcgcctccg cccgccggaa aaacggccac
     1621 agctcgcggt gcgagccgta cggatcggaa aagcagtgct cgtgcaccgg gaacaccttg
     1681 tcgaggatca ctgcgcgaaa gccgttctcc ctcgcccact ccagttcacg gattgctcct
     1741 tccacgtcct gcagcgccac cagcgctccc ccgatcaatt gttccggatg gcgctgcacg
     1801 agcctgagga cggacaggtt gtaggaatgc gccatcgcgt tcgccaggcg cggctcgacc
     1861 aggtacgaga acaccgtggc ggtcaactgc gggaacagga actgccggtc gatgcccagc
     1921 ttctggtaat cccgcaggcg ctcttccata tcgtacatgc cgcggtagcg cgcgccggtg
     1981 ccaggcggct cgagcggggt cgcgcccggc acccgcggcg gcgcgccggg aaaatccacg
     2041 cccgtgagca gccccttgtc gtcccagcgg aagcgcgggc gcctgtcctc ccattccggg
     2101 cccatgtggt cataggcgtc cggcggcagg taatgggtat cgcaatcgat gatcatgatc
     2161 ctgcacgcgc gcggtcagcc cccgacggcc ggcgcatacc gattaacgca aggtaaacgg
     2221 aaaaaattcc cagcaacacg aaccatgcca gcgaccagcc ggcaatcgac aacccgagga
     2281 acgtccagcc ggtctcagcg cattcgccgc tgccacccag aactttctcg atcgcctgcc
     2341 acagcggaaa tttcctgagc atgtattcca acccggggcc gcactccggc acctggtgcc
     2401 gcgggagatg ctgcagccag acgtgacggg cggcgatcgc cgcgccgatt gccgccgtcg
     2461 tgaacagcag cccgccatag acgatcgcgc caagacggcc tggtccgtgc agcgccgcga
     2521 ccagaaacac cgccatcagc gccatgtaca ccaggcgctg caggagacac agggggcacg
     2581 gatcctggta ctcatagtac tggaggtaca gcgcgaaccc gatcagcccg gcgcacgtga
     2641 ggaaaccgag cgcgtaaccg agccgcggca tcaaaggcag gccggcaatc gtccgcatgc
     2701 gacaagttta ccgaaatatt tgacgaccga gtggaaggta tctcgaaaag ccgaacggaa
     2761 ccgcagatga acgcagatga acacggatat ttcgaatgcc gaggaagtca tcagcaaggg
     2821 ctcgcgttcg cgtttatccg tgccaatctg tcgccagttc gccctgcttt accttgaaat
     2881 ccaggatttg gctcacgaat caaaaaaacg gttcgagctg cggataatcc gtgataaacg
     2941 ccgatgcatg gaacgacaaa tcatcgggtt gttttcaata gatttgcgtt caccggcgtt
     3001 catcggcggt tcctgggttt tggacatcat tgagacgggc tctagttgtg cgggccggaa
     3061 gcgcgccgta cactcgactc atgatcagag tggcgcccgg cat
//
LOCUS       DBNGWZ010000672         6617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_626233, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000672 DBNGWZ010000000
VERSION     DBNGWZ010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_626233"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16CX_20955"
     CDS             complement(<1..572)
                     /locus_tag="AC16CX_20955"
                     /inference="COORDINATES: protein motif:HMM:NF013440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR3015011.1"
                     /translation="MHRTPYATDAGESGDFPLHINLGVPMSRAFTKESTDDLVAGELP
                     ERPVSARPNYVTPHGGEQLQARVKALQEQHEKLQAQSEEDTAAKHKLREIERDQRYFN
                     AQLERAIVVDPAGQPRDEVHFGATVKIIDEDGKKHLFVIVGDDEADVGSGKISWASPL
                     AEAMIGARVGDTVKWRRPAGDAEVEIVEIA"
     gene            complement(715..1860)
                     /locus_tag="AC16CX_20960"
     CDS             complement(715..1860)
                     /locus_tag="AC16CX_20960"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR3015012.1"
                     /translation="MEQRNQPRTGLIMTGGGARAAYQVGVLRALSELLPQDVRTPFPI
                     ICGTSAGAINAAVIAADAGDFRRGVRRLMTVWKNFRVPHVYRADPWGALSNSLRWIFA
                     VLTGGVFSKRPVSLLDNTPLAALLGRYLDFSAIQRAIDAGDLTAFSVTCSGYTSGQSV
                     TFFQGVPGLQPWQRARRIGVPMPVTLEHLLASSALPFIFPPVHINREYFGDGSMRQIA
                     PVSAALHLGADRLFVIGVGRQLQQRAERVKVETHPSLAQIAGHALNSIFLDSLEVDLE
                     RMQRINRTIEMIPADALERTNYPLHHVDFRLISPSEELERIAVAHAGELPFTIRSLLY
                     PVGGMRRSGANLLSYLLFERSYCRALIRLGYQDTMERKDDLVAFFGY"
     gene            complement(1982..2626)
                     /locus_tag="AC16CX_20965"
     CDS             complement(1982..2626)
                     /locus_tag="AC16CX_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HIR3015013.1"
                     /translation="MAHRQLTQTQDRLFPLTRERIAEALARPAPPPEPHDRHVIALKE
                     GMRVTEAAVLVPIVNRAGRPQLLLTQRTLHLDDHAGQISFPGGRVEAGDTSREETALR
                     ETEEEIGLARAMVALLGKLPDYEIPSGFRITPVVGWIEPPFTLKPDPFEVAEVFEAPL
                     AHFLDAANYQRREYRFRGRHRHYLAIPFEGRYIWGATAGMLYHLCRVLRGDREP"
     gene            2781..3026
                     /locus_tag="AC16CX_20970"
     CDS             2781..3026
                     /locus_tag="AC16CX_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015014.1"
                     /translation="MKIRSIVVAVSLALASSAAFAFHCPKDMKAIDAALPNAKLSDAQ
                     MSEVKKYRAEGEALHKAGKHQESVDTLAKAMKILNIQ"
     gene            complement(3213..4676)
                     /locus_tag="AC16CX_20975"
     CDS             complement(3213..4676)
                     /locus_tag="AC16CX_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR3015015.1"
                     /translation="MNQKLVVRALVAAGLVTAVAVGGMKIYEPLSAQARAEASAVQIA
                     APAPLIAQALPDFTTLVERYGPAVVNVSVTHTVKTGPDAPQYPGLDPDDPFYEFFRRF
                     QIPIPRGGVPAQGLGSGFIVSPDGLILTNAHVVRDASEVTVKLTDKREFKAKVIGSDP
                     QTDVAVLRIDAKGLPAVALGGSDSVRVGEWVVAIGSPYGFENSVSAGIVSAKGRALPD
                     GTYVPFIQTDVAVNPGNSGGPLFNLKGEVIGINSQIYSRTGGYQGLSFAIPIDLAVKV
                     KDQLVQHGKVTRGRIGVTIQEVNQPLADSFGLKKPAGALVSSVEKGGPAEKAGIEPGD
                     VILRVDGKEIGRSIDLASRIADLRPGTRAKLEVWRKGSPREITVTVGEMKGPKVASGK
                     AGGQDHGRLGLAVRPLTPEERKEAGVSGGLLVEEVNADSAAARAGIQPGDIILALNST
                     PVNSVEQLRGIVVKSGKRAALLVQREDSKIFVPVELG"
     gene            complement(4984..5712)
                     /locus_tag="AC16CX_20980"
     CDS             complement(4984..5712)
                     /locus_tag="AC16CX_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3015016.1"
                     /translation="MSRESKAVMQRVAVVTGANRGIGFEICRQLAKHGGIRVVLTARD
                     EAKGKAAARKLRGEGLDIDSHQLDIASEKSVKEFAGWLGGACKRCDILVNNAGVMLDP
                     RGSRVLDSKVETWRDTLETNLVGPLRLSQALAPLMKKNGYGRIVNVSSGQGQLSDMGV
                     GTPAYRVSKTALNALTCALAADLKGSGILVNSMCPGWVRTDMGGSSAPRTVEQGADTA
                     VWLATLPDDGPTGGFFRDRKPIPW"
     gene            complement(5733..>6617)
                     /locus_tag="AC16CX_20985"
     CDS             complement(5733..>6617)
                     /locus_tag="AC16CX_20985"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3015017.1"
                     /translation="TLDRKAREFARIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLA
                     HVDAALPHLTELALGGTAVGTGLNAHPQFAEKVAAELARLTRLPFVTAPNKFEALASC
                     DAVVHAHGALKTLAASLMKIANDVRWLASGPRSGIGELLIPENEPGSSIMPGKVNPTQ
                     SESMTMVCCQVFGNDVAINLGGASGNFELNVFRPLVIRNFLHSAKLLAHACRNFNEHC
                     AAGIRPNRARIEQLMRDSLMLVTALNPHIGYEKAAAIAKKAHKEGTTLKEAALKLGYL
                     TAKQFDEWVRPEKMVGKI"
BASE COUNT         1099 a         2256 c         2129 g         1133 t
ORIGIN      
        1 taggcgatct cgacgatctc gacctcggca tctccggccg ggcgacgcca cttgaccgtg
       61 tcgccgacgc gcgccccgat cattgcctcg gcaagcggcg aggcccagct gatcttgccg
      121 ctcccgacat cggcctcatc gtcgccgacg atcacgaaca gatgcttctt gccgtcctcg
      181 tcgattatct tcaccgtggc gccgaaatgc acctcgtcgc gcggctggcc ggcggggtcc
      241 acgacgatgg cgcgctcgag ctgggcgttg aaatagcgct ggtcgcgctc gatctcgcgc
      301 agcttgtgct tggccgccgt atcttcctcc gattgcgcct gcagtttctc gtgctgctcc
      361 tgcagcgctt tcacccgcgc ctgcagctgc tcgcccccgt ggggtgtcac gtagttgggg
      421 cgcgcggaaa ccgggcgctc gggcagttcg cccgccacca ggtcgtcggt gctttctttc
      481 gtaaaagccc ggctcatggg caccccgagg ttgatgtgca ggggaaaatc gccggattca
      541 ccggcgtcgg tcgcatacgg cgtccggtgc aatccggcgg attaaccggg cgcatcgcgt
      601 cccggtactt ataccggcag acagggcgct aggcaacagc cgacgcgccg gccgagcggg
      661 gggcgggtag accatgaccg ccgccaaaag ttctaccggt tcatgcagtt gcggtcagta
      721 gccgaagaac gccaccagat cgtccttgcg ctccatcgtg tcctgatagc cgagccggat
      781 cagggcgcgg cagtaggatc tctcgaacag caggtacgag agcaggttgg cgccgctgcg
      841 ccgcatgccc cccacggggt agagcagcga gcggatggtg aacggcagct cgcccgcgtg
      901 cgccacggca atccgctcga gctcctcgct cggagatatc aggcggaagt ccacgtggtg
      961 cagggggtag ttggttctct cgagggcgtc cgccgggatc atctcgatgg tgcggttgat
     1021 gcgctgcatc cgctcgagat ccacctccag gctgtccagg aagatgctgt tcagcgcgtg
     1081 gcccgcgatc tgcgcgagcg aaggatgggt ctcgaccttg acgcgctccg ccctctgctg
     1141 gagctggcgt cccaccccga tcacgaacag gcgatcggcc cccaggtgca gggcggcgct
     1201 caccggggcg atctgccgca tcgagccgtc gccgaaatac tcgcggttga tgtgcaccgg
     1261 cgggaagatg aacggcagcg cgctggaagc gagcaggtgc tcgagcgtga ccggcatcgg
     1321 cacgccgatg cggcgcgcgc gctgccacgg ctgcagcccc ggcacgccct ggaagaaggt
     1381 caccgactgg cccgaggtat agcccgagca ggtcacgctg aaggcggtga gatcgccggc
     1441 gtcgatcgcg cgctggatcg cggaaaagtc gagatagcgc ccgagcaggg cggcgagcgg
     1501 cgtgttgtcg agcagcgaca ccggccgctt cgaaaagacg ccgccggtca gcaccgcgaa
     1561 aatccagcgc aaactgttcg agagcgcgcc ccacggatcc gcgcgataga cgtgaggcac
     1621 gcggaagttc ttccaaaccg tcatcagccg ccgcacgccg cgacggaagt cgccggcgtc
     1681 cgccgcaatc actgcggcgt tgatggcgcc ggcggaagtg ccgcagatga tggggaaggg
     1741 cgtgcgtacg tcctgcggca ggagctccga gagcgcccgc aacacgccga cctggtaggc
     1801 ggcgcgcgcg ccgccgccgg tcatgatcag ccccgtccgg ggctgatttc tttgctccac
     1861 tgtccctccc ctgccgtacc cgctctttat agtaagcggt aagcggtaag cggtgaacgg
     1921 taaacaacca agacccccct accgggggcg gctcgatctt ttcgccctca cggcttactg
     1981 ctcacggctc acggtccccg cgcagcacgc ggcaaaggtg gtagagcatg cccgccgtcg
     2041 cgccccagat gtagcggcct tcgaacggga tcgccaggta atggcgatga cggccgcgga
     2101 agcggtattc gcggcgctgg tagttggcgg cgtcgaggaa atgcgcgagc ggcgcctcga
     2161 acacctcggc cacctcgaac gggtcgggct tcagcgtgaa cggcggctcg atccagccca
     2221 ccaccggcgt gatgcggaag cccgacggga tctcgtaatc ggggagcttt cccagcagcg
     2281 ccaccattgc gcgcgcaagc ccgatctcct cctcggtctc gcgcagcgcc gtctcctccc
     2341 ggctcgtgtc gcccgcctcc acccgcccgc cgggaaaact gatctgcccc gcgtgatcgt
     2401 cgaggtgcag cgtgcgctgc gtgaggagca gctgcggccg gccggcgcga ttgacgatcg
     2461 gcaccagcac cgccgcttcg gtgacgcgca tcccctcctt cagcgcgatg acgtgccggt
     2521 cgtgcggctc cggcggtggc gccggccgcg cgagcgcctc ggcgatgcgt tcccgtgtca
     2581 gcgggaataa ccggtcctgc gtttgtgtta actgtcggtg agccacgtcg tattcgctaa
     2641 cgccctggaa tggaaaggaa ttctactcgc gggcggcagg cagcgcatcc ggcggcatcc
     2701 gacgcgcgta acagagcgtg gcgccggtaa ccggcttacg gtcgacaaaa ccgcttttca
     2761 acccgaaaga ggagaatcgc atgaaaatcc gcagcatcgt ggtcgcagtc agcctcgccc
     2821 tggcgagcag cgcggcgttc gctttccatt gcccgaaaga catgaaggca atcgacgcgg
     2881 cgttaccgaa tgccaagctc tcggacgcgc agatgtcgga agtgaagaaa taccgcgcgg
     2941 aaggcgaagc gctgcacaag gcgggcaagc atcaggagtc ggtggacacg ctcgcgaagg
     3001 cgatgaaaat ccttaatatc cagtaagcgc cagcattggg gaccgcgact tgctggctgc
     3061 actgatgtcg cgggatttca gcgtagggta acctaactat tcttctggtt agccggaact
     3121 aaaatcctga acatccagta aacgcgcacg ccctcccaaa aatccccgcc ctcgcggcgg
     3181 ggattttttt gcccataaac cgtgccgcgg cgtcagccga gttcaaccgg cacgaagatc
     3241 ttcgagtcct cgcgctgcac cagcagggcc gcgcgcttgc ctgacttcac gacgatgccg
     3301 cgcagttgct cgacgctatt caccggcgtg ctgttcagcg cgagtatgat gtcgcccggc
     3361 tgtatgcccg cccgcgccgc ggcgctgtcc gcgttcacct cctcgaccag cagcccgccg
     3421 ctcacgccag cctctttccg ctcctccggc gtgagcgggc gcacggcgag tccgaggcgg
     3481 ccgtggtcct gcccgccggc cttgcccgaa gcgaccttgg ggcccttcat ctcgcccacg
     3541 gtgacggtga tctcgcgcgg tgagcccttg cgccacactt cgagcttcgc ccgggtcccg
     3601 ggcctcaggt ccgcgatgcg gctcgccagg tcgatcgagc ggccgatttc cttgccgtcg
     3661 accctgagga tcacgtcgcc gggctcgatg cccgccttct cggccgggcc acccttttcc
     3721 accgagctca cgagcgcccc cgccggcttt ttgagaccga aggaatcggc aagcggctgg
     3781 ttcacttcct ggatggtgac gccgatgcgg ccacgggtca ccttgccgtg ctggaccagc
     3841 tggtccttca ccttcacggc aaggtcgatc gggatcgcga acgacaggcc ctggtagcct
     3901 ccggtgcggc tgtagatctg cgagttgatg ccgatcacct cgcccttcag gttgaacagc
     3961 ggcccccccg agttgccggg attgacggca acatcggtct ggatgaacgg cacataggtg
     4021 ccgtcgggca gcgcccgccc cttggcgctc acgatacccg cgctcaccga attctcgaag
     4081 ccgtagggcg agccgatcgc caccacccat tccccgacgc gcacgctgtc cgagccgccg
     4141 agcgcaaccg ccggcaggcc cttggcatcg atcctcagca ctgcaacgtc agtctgagga
     4201 tcggaaccga tgactttcgc cttgaattcg cgcttgtcgg tcagcttgac ggtaacctcg
     4261 gaggcatccc gcaccacgtg cgcgttggtg agaatcagcc cgtcggggct cacgatgaag
     4321 ccggagccaa gtccctgcgc cggcacgccg ccgcgcggaa tcgggatctg gaaacgcctg
     4381 aagaactcgt aaaacgggtc gtcgggatcg agtccagggt actgcggcgc atcagggccc
     4441 gtcttcaccg tgtgcgtcac gctgacgttg accacggcgg gcccgtagcg ctcgaccagc
     4501 gtcgtgaaat cgggcaatgc ctgcgcgatg agcggcgccg gcgcggcgat ctgcacagcg
     4561 ctcgcctcgg cgcgcgcctg agccgacaac ggctcgtata tcttcatgcc tccgaccgcg
     4621 acggcggtga cgaggccagc ggcgaccaaa gctcgaacaa cgagtttctg attcatgctc
     4681 ctctccatag aattgaaccg gcctgaacgg gtcgtacggg accgcgtggc cgcaagcgcg
     4741 ctccgccccg gcaggcgcag cagcgcgccg ggtgtccggc ccgaaaaaac gactacgccg
     4801 cttttgaccg cgaagcccgc gccgggtttc gtcgggtagc ttggtcgcgc gccgtatctg
     4861 tcagggaact tttttcaggg gagccgctcg ccgtgccgcg agtacggccc ggacatcagg
     4921 agaaaccgcc acacttgccg gtcacgggtg accagtcacg agtcaccagt cacaagtcac
     4981 gcttcaccaa ggaatgggct tgcggtcgcg gaagaagccg cccgtgggcc cgtcatccgg
     5041 cagcgtcgcc agccacaccg ccgtatcagc gccctgctcc accgtgcgcg gggccgaaga
     5101 tccgcccatg tcggtcctca cccaccccgg gcacatcgag ttcaccagta tcccgctgcc
     5161 cttgagatcc gcagcgagcg cgcaggtgag cgcattgagc gccgtcttcg atacgcggta
     5221 ggccggcgtg ccaacgccca tgtccgagag ctggccttgg ccgctggaaa cgttgacgat
     5281 gcgcccgtag ccgtttttct tcatgagcgg cgcgagcgcc tggctcagcc tgagcggccc
     5341 gacgagattg gtctcgagcg tgtcgcgcca ggtctcgacc ttcgaatcga gcacgcgcga
     5401 gccgcgcggg tcgagcatca cgccggcgtt attgacgagg atgtcgcaac gtttgcaggc
     5461 gccccccagc cagcctgcga actctttgac gctcttttcg cttgcgatat cgagctgatg
     5521 gctatcaatg tcgagccctt cgccgcgcag cttccgtgca gccgccttac ctttcgcctc
     5581 atcgcgggcg gtcaggacga cacggatgcc gccgtgcttc gcaagctgcc ggcagatttc
     5641 aaacccgatg ccccggttcg cgccagtgac aaccgccacc cgctgcatca cggctttcga
     5701 ctcacgactc accactcacg actcacgggt ttctagatct tgccgaccat cttctccggc
     5761 cgcacccact cgtcgaactg tttcgcggtg aggtaaccga gcttcagcgc cgcttccttc
     5821 agcgtcgtgc cttccttgtg cgccttcttg gcgatggcgg cggccttttc gtagccgata
     5881 tggggattca gcgccgtcac cagcatcagc gaatcgcgca tgagctgctc gatgcgggcg
     5941 cggttcgggc ggatgccggc ggcgcagtgc tcgttgaagt tccggcaggc gtgcgcgagc
     6001 agcttcgcgc tgtgcaggaa attgcggatc acgagcggcc ggaacacgtt cagctcgaaa
     6061 ttgcccgagg cgccgcccag gttgatcgcg acgtcgttgc cgaacacctg gcagcacacc
     6121 atggtcatgg actcggactg ggtggggttg accttgcccg gcatgatcga gctgcccggc
     6181 tcgttctccg ggatcaagag ctcgccgatg ccgctcctcg ggcccgaagc gagccagcgc
     6241 acgtcgttcg cgatcttcat gaggctcgcc gcaagcgtct tcagcgcgcc gtgcgcgtgc
     6301 accaccgcgt cgcaggaggc gagcgcctcg aacttgttcg gcgcggtgac aaacggcagg
     6361 cgcgtcagcc gcgcgagctc cgccgccacc ttctccgcga actgcggatg cgcgttcagc
     6421 ccggtcccga ccgcggtgcc gcccaaagca agctcggtga gatgcggcag cgccgcgtcc
     6481 acgtgggcga ggccgtggtc gagctgcgag acgtagcccg agaattcctg tcccagcgtg
     6541 agcggcgtcg cgtcctgcag gtgggtgcgg ccgatcttca cgatcctcgc gaactcccgc
     6601 gccttccggt cgagcgt
//
LOCUS       DBNGWZ010000673         7231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3145472, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000673 DBNGWZ010000000
VERSION     DBNGWZ010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3145472"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="AC16CX_20990"
     CDS             <1..1572
                     /locus_tag="AC16CX_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR3015018.1"
                     /translation="FERAGRLFPESEIVSRMPDKSLHRYKYADFYRRAQALISALRKL
                     GLKRGERVATLMWNHHVHLEAYFAIPCVGGVLHTLNLRLSPDDIAFIANHAQDRFLIV
                     DDVLLPLYDKIRASTNFEKVIVVPLTGSSVPAGLENYETLISRPRPVRLPRLKEEDPA
                     GMCYSSGTTGRPKGVVYTHRALVLHSLASALPDVMGMGNHDTVTPVVPMFHVNAWGIP
                     FTAVMVGAKLAFPGPHLDAVSLLDLYQSEQVTLTAGVPTIWAGILQALEKEPSRWKLP
                     AGMRMVVGGAAAPESMIRAFDRHNLKVIHGWGMTETTPVAAVNYLKREIADTNSDEQY
                     ALRAKQGVPPPFVEVRAMTGQGEAPWNGMTMGELEVRGPWVAAGYHDLDSESDKWSED
                     GWFRTGDVVTIDAHGYIKITDRIKDLVKSGGEWISSVDLENALVGHPKVAEAAVIAVP
                     DPKWGERPLAVVVLKPGQSATQDELREHLAPKFAKFWLPEAFEFVPEIPRTSTGKMLK
                     AKLREQFKDWKPGGR"
     gene            1781..2383
                     /locus_tag="AC16CX_20995"
     CDS             1781..2383
                     /locus_tag="AC16CX_20995"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR3015019.1"
                     /translation="MGKRKLEKAIGLYRERHPGGPEPAVRWLPFQLNPDLPESGIPRE
                     EYVARKFGARGKAVSDRVTMAGRQVGIPFAYEKMKVQPNTLNAHRLMLYAERAGKQDE
                     LAEELFRAHFIEGASVADREMLADIAARAGLDRKAVAGYLAGDEDRARIAQADVEARS
                     AGIGGVPFFIFNRKVGISGAQDPETLLNAMEEALRDSEES"
     gene            complement(2464..3090)
                     /locus_tag="AC16CX_21000"
     CDS             complement(2464..3090)
                     /locus_tag="AC16CX_21000"
                     /inference="COORDINATES: protein motif:HMM:NF012342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HIR3015020.1"
                     /translation="MSFFHRLFREHWGFTLIFGFLWFVVIAFSMIGPEDQRHPNPGPL
                     LMLLTASTILVSFAWMNSKHFQQAEGAFKRHALTWGTALLDFAVFAFLFALPAAILAP
                     AYGNYAIRARMSEMISSVSALRTSIADVAQSKGTLSGAAAGLQAGTHKHADYALLSPD
                     GTIVAYSERYGTLVVLTPTLSGGSVSWRCQGYPERLFPVACRRGAKQG"
     gene            3284..4993
                     /locus_tag="AC16CX_21005"
     CDS             3284..4993
                     /locus_tag="AC16CX_21005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3015021.1"
                     /translation="MPETTTYRNALQLQSMLLEYRLESVLGAGGFGMTYLGWDTNLEK
                     HVAIKEYLPTDLAVRALDGSIVPITTELKYNYQWGLERFILEARTLAKFSHPHIVRVN
                     RYFEANSTGYMVMDYEKGESLNQMLKRGQPPDEAKLKSILMPLLDGLQAVHATGFLHR
                     DIKPSNIFIRESGSPVLIDFGASRQAVGGATKSLTSVLTPGYAPLEQYSSDGHQGPWS
                     DIYAMAGVLYRSLAGDNPPDAVTRLKSDTVPAKLTTLRGRVSEPFLRGMEWALTLDEK
                     QRPQSVEDWKRALEGKAALPAAAAARTASIVVATRPATAAPTQTAPTQLLPDSQPQRP
                     LSRPPRPLPEEEPRSGWRWLGIGALALVAVFAYSAWNKHRQAGQLAAEQRAAEQRAAK
                     QQEAERLAEERRAAEQSRFELQEAREREAQQQRLAERDARMSDAEQQRLAEEREARLR
                     EAERRAAEVEAALRRVEGERARERAAAAGSPSEAGRERPSREREERPGGPDPEEMRRR
                     AEQDFRAADSNADGYLTRDEVQGRMPFVAKEFHRIDADGDGRISREEFMRFRKEMFQR
                     FKK"
     gene            complement(5070..5456)
                     /locus_tag="AC16CX_21010"
     CDS             complement(5070..5456)
                     /locus_tag="AC16CX_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR3015022.1"
                     /translation="MSMLDTIAERRIREAQARGEFDDLPGAGAPLELDDDALVPEELR
                     VAYRMLKNAGFVPPEIEAHRELREIEQLLQAAEDEVERARLFSLINFLLARSGAGRRR
                     GNLRVEQAYLEQIAERLGRTRGRRDE"
     gene            complement(5558..6415)
                     /locus_tag="AC16CX_21015"
     CDS             complement(5558..6415)
                     /locus_tag="AC16CX_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3015023.1"
                     /translation="MKSDSVLGLSSKGFHRIHYTDWGDPDAERIVICVHGLSRNCRDF
                     DFLAQALAPDFRVVCPDVVGRGKSDWLYAKEDYGYPQYCADMAALIARVTAGDKPRKI
                     YWVGTSMGGIIGMLLASRPRTPIEKLVLNDVGTVIPKAALGRIAMYIGKDPRFKTYQE
                     LEAMVRMVSAPFGPLTDAQWRHLTVTGAKQYEDGSWGVCYDPGIGIPFQKGPLADVDL
                     WSFWDTIACPTLLLRGAQSDLLLKEVAMQMTQRGPKPRLVEFEGIGHAPMLMAEDQIK
                     VVRDFLLSG"
     gene            complement(6426..>7231)
                     /locus_tag="AC16CX_21020"
     CDS             complement(6426..>7231)
                     /locus_tag="AC16CX_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015024.1"
                     /translation="EVWPFALIAMSVVSFFAWAANYRRYRQIQDLPTSKVVSAAQGYV
                     ELFGKSDMLPGVPVQSCLSKSSCCWYAYEVEEKDSKDKWHTVDSGKSTEQFLLVDDTG
                     QCVISPDGAEVLTHDHKSWTEGDHRYNEWLLRPQCALYALGEFSTTTAAAVAARDERA
                     DTGALLVEWKQDQKRLLGRFDLNRDGKIDMKEWELARLQAQREVRKRQAEMQSRSVDG
                     VNLLHKPSDGRLFLLANEVPDKLGSRYRFWSWVHLVIFIGAGSAGLFML"
BASE COUNT         1392 a         2270 c         2339 g         1230 t
ORIGIN      
        1 ttcgagcgcg cgggcaggct cttccccgag tccgagatcg tctcgcgcat gccggacaag
       61 tcgctgcatc gctacaagta cgcggacttc taccgtcgcg cgcaggcgct catctccgcg
      121 ctgcgcaagc tgggcttgaa gcgcggcgag cgcgtcgcga cgctcatgtg gaaccatcac
      181 gttcacctcg aagcctactt cgcgattccg tgcgtcggcg gggtgctgca cacgcttaat
      241 ctccgcctca gtcccgatga cattgcattc atcgcgaacc acgcgcagga ccgcttcctg
      301 atcgtggacg acgtgctgct gccgctctac gacaaaattc gtgcgagtac gaattttgag
      361 aaggtcattg tggtgccact tacaggatcc tccgttccgg caggattgga gaactatgaa
      421 accctgatat cgcgtccccg gcctgtgagg cttcccagac tgaaggagga ggatcctgct
      481 ggcatgtgtt acagctccgg gacgaccggc cggccgaagg gtgtcgttta cacccaccgc
      541 gcgctggtgc tgcactcgct cgcctcggcg cttcccgacg tgatgggcat gggcaaccac
      601 gatacggtga cgccggtggt accgatgttc catgtcaacg cctggggcat tccgttcacc
      661 gcagtgatgg tcggcgcgaa gctcgctttt cccgggcctc atctcgacgc ggtaagcctg
      721 ctcgatctgt accagtccga gcaagtgacg ctcaccgcgg gcgtgcccac gatctgggcc
      781 ggtatcctcc aggcgttgga gaaggaaccg tcacgctgga agcttcccgc cggcatgcgc
      841 atggtggtgg gcggcgcggc ggctcccgaa tccatgatcc gcgccttcga ccgccacaac
      901 ctgaaagtca tccacggctg gggcatgacc gagaccacgc cggttgcagc cgtgaactac
      961 ctgaagcggg aaatcgccga tacgaacagc gacgagcaat atgccctcag ggcgaagcag
     1021 ggcgtgccgc ccccattcgt ggaagtgcgc gccatgaccg gccagggcga ggcgccgtgg
     1081 aacggcatga ccatgggcga gctcgaagtg cgcggtccgt gggtcgcagc gggctatcac
     1141 gatctcgatt cggagtccga caagtggagc gaggacggat ggttccgcac cggcgacgtc
     1201 gtcaccatag acgcgcacgg ctacatcaag atcaccgacc gcatcaagga cctggtcaag
     1261 tcgggcggcg aatggatcag ctcggtggac ctcgaaaatg cgctggtcgg gcaccccaag
     1321 gtagcggagg ccgccgtgat tgcggtgccg gacccgaagt ggggcgagcg gccgctggcg
     1381 gtggtggtgc tgaagccggg gcagtcggcc acccaggacg agctgcgcga gcacctcgcg
     1441 ccgaagttcg cgaagttctg gctgccggag gcgttcgagt tcgtgccgga aatcccgcgc
     1501 acctcgacgg ggaagatgct gaaggctaaa ctgcgggaac aattcaaaga ttggaaaccg
     1561 ggagggcggt agagcgcgag ggcgggaggg cgacaaaagc cgtgagggcg ggagagcgtg
     1621 agagcgagag agagacaatc catctcccga tctcccgatt tcacgatttc ccggtttttc
     1681 gatctcccga tctcacgacc tcccgatctg ggatgagcat atgagcgaaa agacgctgca
     1741 gatagacata gtctcggatg tagtttgacc ctggtgcttc atcgggaagc gcaagctcga
     1801 aaaggcgatc ggcctttacc gcgagcgcca tcccggtgga cccgagccgg ccgtgcgctg
     1861 gctgcccttt cagctcaacc cggaccttcc cgagagcggc attccgcgcg aggaatacgt
     1921 tgcgcgcaag ttcggcgcgc gcggcaaggc cgtctccgac cgcgtgacga tggcgggccg
     1981 gcaggtcggc atcccgttcg cttacgagaa gatgaaggtc cagcccaaca cgctcaacgc
     2041 gcaccggctg atgctctacg cggagcgcgc tggaaagcag gatgagctgg cagaggagct
     2101 gttccgcgcc cacttcatcg agggcgccag cgtcgcggac cgggaaatgc tggccgacat
     2161 tgccgcgcgc gcgggcctcg accgcaaggc ggtggccggc tacctggctg gcgatgaaga
     2221 ccgcgcgcgg atcgcccagg ctgacgtcga ggcccggagc gcaggcatcg gcggcgtgcc
     2281 gttcttcatc ttcaaccgca aagtcggcat ttcgggcgcg caggacccgg agacgctgct
     2341 gaacgcgatg gaggaggcgt tgcgggacag cgaggagtct tgaaggcaga aggcagaagg
     2401 cagaaggcag aaggaagtga tgggctacta cacctaactg ttttttcctt tatccttctt
     2461 ccttcatcct tgttttgctc cgcggcggca ggcgactgga aacagtctct ccggatagcc
     2521 ctggcagcgc cagctgacgc tgccgccgct cagcgtagga gtcagcacaa ctaaagtgcc
     2581 gtagcgctcg ctgtaggcca cgatggtgcc gtcagggctg agcagagcgt agtcggcatg
     2641 cttgtgcgtt cccgcctgga gtcccgctgc ggctccgctc aacgtgccct tcgactgcgc
     2701 cacgtcggct atcgaggtcc tgagcgcgga gacggaagaa atcatctcgc tcatgcgagc
     2761 gcgaatcgcg tagtttccat atgcaggcgc taggatcgcg gcgggaagcg cgaacaggaa
     2821 agcaaacacc gcaaaatcga ggagcgcggt tccccacgta agcgcgtgtc ttttgaatgc
     2881 accctccgcc tgctggaaat gcttcgaatt catccaggca aagctcacca gaatcgtcga
     2941 cgccgtcaac agcatcaaca gcggcccggg attcggatga cgctgatcct cgggcccgat
     3001 catcgagaaa gcgatgacca cgaaccacaa gaagccgaaa atcaaggtga agccccaatg
     3061 ttcccggaac agcctgtgaa aaaacgacat ggctcgcgct cctgtgtgag accccaacgc
     3121 ggtatcttag cgttctatcc cgctgtcatc tatcatatgc ggagcacggg aggggtgagc
     3181 gaggaggaaa aggcgtgata agtgacaagt gataagaaga accagcttct tcggtcttca
     3241 gacctatcac ctatcactta tcacctatca cgcctcttca agcatgccgg aaaccacgac
     3301 ctaccgcaac gcgctgcaac tgcagtcgat gctgctcgag taccggctgg agtcggtgct
     3361 cggcgcgggc ggtttcggca tgacctatct cggctgggac accaacctcg agaagcacgt
     3421 cgcgatcaag gagtacctgc cgaccgatct cgccgtgcgc gcgctcgacg gcagcatcgt
     3481 ccccatcacc accgagctta agtacaacta ccagtggggc ctcgagcgct tcatcctgga
     3541 ggcgcgcacg ctcgcgaagt tcagccaccc ccatatcgtc cgggtgaacc gctacttcga
     3601 ggcgaacagc accggctaca tggtgatgga ctacgagaag ggcgaatcgc tcaaccagat
     3661 gctgaagcgc gggcagcccc cggacgaagc gaagctgaag agcatcctga tgccgctgct
     3721 cgacggcttg caggcggtgc acgccacggg ttttctacat cgcgacatca agccctctaa
     3781 catcttcatc cgcgagagcg gcagcccggt gctgatcgac ttcggcgcgt cccggcaagc
     3841 agtcggcggc gccaccaaga gcctcacctc ggtgctcacg ccgggctacg cgccgctcga
     3901 gcagtattcg agcgacggcc accagggtcc ctggtccgac atctacgcga tggccggggt
     3961 gctctaccgc tcgctcgccg gcgacaatcc gccggacgcc gtgacccggc tgaagagcga
     4021 caccgtgccc gcaaagctca cgacgttacg cgggcgggtg agtgaaccgt tcctgcgcgg
     4081 aatggaatgg gcgctcacgc tcgacgagaa gcagcgcccg caaagcgtgg aggattggaa
     4141 gcgcgcgctg gaaggaaagg ccgcgctccc agcagcagca gcagcacgta cagcgtccat
     4201 tgtggtggcg acgcggcccg ctacggcggc gcctacccag acggccccca cgcaactgct
     4261 gccggacagc cagcctcagc ggccgttatc gcgcccgccg cggccgctcc cggaggagga
     4321 gcccaggtcc ggctggcggt ggttggggat cggcgcgctc gcgctggtcg ccgtctttgc
     4381 ctacagcgcg tggaacaagc accggcaggc ggggcagctg gcagccgagc agcgggccgc
     4441 ggaacagcgc gctgcgaaac agcaggaagc cgagcgtctg gcggaggaaa ggcgcgcggc
     4501 ggaacagagc aggttcgagc tgcaggaggc acgggagcgc gaggcacaac agcagagact
     4561 ggcggaacgc gacgcgcgca tgagtgacgc ggaacagcag cggcttgccg aggaacgcga
     4621 ggcacggctt cgtgaagcgg aacggcgcgc cgccgaggtc gaggccgcgc tcaggcgtgt
     4681 cgaaggggag cgcgcgcggg aaagggccgc cgcggccggg tcaccatccg aagccgggcg
     4741 cgaacgcccc tcgcgcgagc gcgaggagcg gccgggaggg ccggatcccg aggaaatgag
     4801 acgccgcgcc gagcaggact ttcgtgcagc ggacagtaat gctgatggct atctcacccg
     4861 cgatgaagtc cagggacgca tgccgttcgt tgcgaaggaa ttccaccgca tcgacgcgga
     4921 cggcgacgga cgcatctcgc gggaagaatt catgcgcttc cgcaaggaga tgttccagcg
     4981 cttcaaaaaa tagccgaaaa gcgtgatagg tgataagtga taggagctga ctcccgcgca
     5041 agcgcgggtg acccatcact cattacacat cactcatcac gccttccgcg cgttcgcccg
     5101 aggcgctcgg cgatctgctc caagtacgct tgttcaacgc ggagattccc ccgtcgtctg
     5161 ccggcgccgg agcgggcgag cagaaagttg atgagggaga acaggcgagc gcgctcgact
     5221 tcgtcttcag ccgcttgcaa cagttgctcg atttcccgta attcgcgatg cgcttcgatc
     5281 tcgggcggca caaaacccgc gttcttgagc atgcgatagg cgacgcgcaa ctcctccgga
     5341 accagcgcat catcgtcgag ttcgagcggg gcgccggcgc cgggcaaatc gtcgaactcg
     5401 ccgcgcgcct gggcttcgcg tatgcgccgc tcggcaatag tgtccagcat cgacattgtg
     5461 gtttgccttt ttcgatgaac gtggtgtcct gtcggactca gtgttaccaa tcctcatcca
     5521 cacttgaggt ccggccttga tttccttctg gcaggcgtta gcctgacaga aggaaatcgc
     5581 gcacgacctt gatctgatcc tcggccatca gcatcggcgc gtggccgatc ccttcgaatt
     5641 cgactagcct gggtttcggg ccgcgctgtg tcatctgcat tgcgacctct ttgagcagca
     5701 gatcggactg cgcgccgcgc aggagcaaag tggggcaggc gatcgtgtcc cagaagctcc
     5761 acaggtccac gtcggcgagc ggccctttct ggaacgggat gccgattccc gggtcgtagc
     5821 acacgcccca ggaaccgtcc tcatactgtt tcgcgccggt cacggtgagg tgccgccact
     5881 gcgcgtcggt gagcgggccg aacggcgccg acaccatgcg caccatggcc tccagctcct
     5941 ggtaggtctt gaagcgcggg tccttgccga tgtacatcgc gatgcgcccg agcgcggcct
     6001 tgggaatgac cgtcccgacg tcgttcagca ccagcttttc gatcggcgtc ctggggcgcg
     6061 aggcgagcag catgccgatg atgccgccca tcgaggtgcc gacccagtag atctttctcg
     6121 gcttgtcgcc ggccgtgacg cgcgcaatca gtgccgccat gtcggcgcag tactggggat
     6181 agccgtagtc ctccttggcg tagagccagt cgctttttcc gcgcccgacg acgtcggggc
     6241 acaccacgcg aaaatcgggc gcgagcgcct gcgcgagaaa gtcgaagtcg cggcagttgc
     6301 gcgagagtcc gtgcacgcag atgacaatgc gctccgcgtc cggatcgccc cagtcggtgt
     6361 agtggatgcg gtgaaaaccc ttgctgctga ggccgaggac ggaatcgctt ttcatcgatg
     6421 ctagtttaaa gcatgaacag cccggcgctg cccgcgccga tgaaaatgac gaggtgcacc
     6481 cagctccaga accggtagcg cgaccccaac ttgtccggca cttcgttggc gagcaggaac
     6541 agccggccgt cgcttggctt gtgcaggaga ttgacgccat ccaccgagcg cgactgcatc
     6601 tccgcctggc gtttgcgaac ctcgcgctgc gcctgcagcc gcgcgagctc ccattccttc
     6661 atgtcgatct tgccatcgcg attgaggtcg aagcgcccga ggagccgctt ctggtcctgc
     6721 ttccattcga cgagcagcgc gcctgtgtcc gcgcgctcat cgcgggcggc aaccgccgcg
     6781 gccgtggtgg tgctgaattc gcctagggcg taaagcgcgc actggggccg cagcagccat
     6841 tcgttatatc ggtggtcgcc ttctgtccat gatttgtggt catgggtgag cacttccgca
     6901 ccgtccgggg aaatgacgca ctggccggtg tcgtccacca gcaggaactg ctccgtgctc
     6961 ttgccgctgt ccacggtgtg ccacttgtcc ttcgagtcct tttcctcgac ctcgtaggca
     7021 taccagcaac aggacgactt gctcaggcag ctctggaccg gcacgccggg cagcatatcc
     7081 gatttgccga acagctcgac atagccttgc gctgcggata cgaccttgga agtgggcagg
     7141 tcctggatct gccggtagcg gcggtagttc gcggcccagg cgaaaaagct caccaccgac
     7201 atggcgatca gcgcgaaggg ccagacttcg g
//
LOCUS       DBNGWZ010000674         3109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_749065, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000674 DBNGWZ010000000
VERSION     DBNGWZ010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_749065"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="AC16CX_21025"
     CDS             <1..811
                     /locus_tag="AC16CX_21025"
                     /inference="COORDINATES: protein motif:HMM:NF014542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3015025.1"
                     /translation="ADLDGAQCTLEEREEYEPHIAAGNVVFAGIDYARIIERAQPEAR
                     LILWDGGNNDFPFLRPDLHIVLVDPLRPGHETTHHPGEAVLRMADIVVVAKTDAAPAS
                     DVQRVADTARRINPRAPLVYAASPVRLDDPLAVRGRRVLVVEDGPTTTHGGMPYGAGY
                     VAAMRAQAAAIVDPRPYATAEILAVYRDYPHIGPVLPATGYTPEQLDGLRRTIDAAPV
                     DVVIAATPIDLGAALAVDKPIVRARYEFEEVAGAGLSAHVERFLQRAGLPA"
     gene            926..2158
                     /locus_tag="AC16CX_21030"
     CDS             926..2158
                     /locus_tag="AC16CX_21030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3015026.1"
                     /translation="MASAFVRALAQFTAPVALLAAAFAIVFYSAALPPSLSGLKVYGP
                     YIVFALGAALAMAFNRGRALLSLIVLAGAYGAQQAWLQQGLEALTARAVYAGLTVFVP
                     LNLAAFAVLPERGTFSRQGALRLGVIGLQAVLVVWAATAGEVGVVDWAYERFLDPAPV
                     SAGRIPQLGIALISLGLLLTMAAALVTRSATPAGLAGAIVAFAIAAHVPTASLTFSVF
                     TAMAELMVTIAVLQDTFRMAFRDELTGLPSRRALNERLDTLGKSYAIAMVDVDHFKKF
                     NDTYGHDLGDQVLKMVAAHISGVGGGGKAFRYGGEEFTILFPGKETAEAIPYLEALRG
                     EIESYRLALRGSDRPKKTKGDKRQRGGWRGKDSVSVTVSVGVAERNDRLVTPQAVIEA
                     ADRALYRAKEKGRNRVSR"
     gene            2244..>3109
                     /locus_tag="AC16CX_21035"
     CDS             2244..>3109
                     /locus_tag="AC16CX_21035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3015027.1"
                     /translation="MNVPLSKQPWFDAALAWYFVTVWGSGFIATKIGLQYAPPFTFLT
                     LRFAFGLACLVPILLIMRPRLPRSRAEFGHVVIAGLLMHAVHLGGSHYTQYLGMSAGI
                     TALMLSAQPLITALIAARWLGERPAARQWAGVMIGLAGVALIVWHKVDVREATLGSLV
                     AVTVSLTGVTAGTLYQRIYCPLVDLRSAAFIQFIATIAALAPLAWLAEGFTVTWAWPM
                     LGSIAFLVIGASILAVSALHTLMRRGQATRVTSLLYLTPIFAVVLELAMFGVVPSALS
                     VAGIAVTCCGVAL"
BASE COUNT          419 a         1119 c         1046 g          525 t
ORIGIN      
        1 ggccgatctc gacggcgcgc agtgcacgct cgaggagcgg gaagagtacg aaccgcacat
       61 tgcggcggga aacgtggtat tcgcgggcat cgactacgcg cgcatcatcg agcgtgccca
      121 gccggaggcg cgcctgatcc tgtgggacgg cggcaacaac gacttcccgt tcctccggcc
      181 cgacctgcac atcgtgctgg tggacccgct gcgcccggga cacgagacca cccatcatcc
      241 cggcgaggcc gtgctgcgca tggcggacat cgtggtcgtc gccaagacgg atgcggcgcc
      301 cgcctccgac gtgcaacgcg tcgcggatac ggcgcgccgc atcaacccgc gcgcgcccct
      361 ggtgtacgcc gcctcgccgg tccgcctcga cgaccccctc gccgtccgcg ggcgccgcgt
      421 gctggtggtg gaggacgggc ccaccaccac tcacggcggg atgccttacg gcgccggcta
      481 tgtcgcggcc atgcgcgcgc aggccgcggc gatcgtggat ccacggccct atgccacggc
      541 ggaaatcctc gccgtgtacc gggactatcc ccacatcggg ccggtgctgc cggcgaccgg
      601 ctacaccccg gagcagctcg acgggctgcg acgcaccatt gatgccgcgc ccgtggacgt
      661 cgtaatcgcg gccacaccca tcgatctcgg cgcggcgctc gcggtcgaca agccgatcgt
      721 gcgcgcgcgt tacgaattcg aggaagttgc cggcgccggc ctgtcggccc acgtggagcg
      781 ctttctgcag cgcgccggat tgcccgcctg acgctcgcat cctctagacg cggggggagg
      841 gaatcaatcc cggaccggcg ctctcgttat aatcccgtta tgctcgggca ccgccacgtt
      901 cccgccccgc cgggccgcgc cgcctttggc tagcgcgttc gtccgggcgc tcgcccaatt
      961 caccgccccc gtcgcgctgc tcgcggcggc tttcgccatc gtcttctaca gcgcggcgct
     1021 gccgccttcc ctctccgggc tcaaggttta cggcccgtac attgtgttcg cgctgggcgc
     1081 cgcgctggca atggcgttca accgcggccg cgcgctgctc tctttgatcg tgctggcggg
     1141 cgcgtatggc gcgcagcagg cctggctgca acaagggctc gaggcgctca cggcacgagc
     1201 cgtgtacgcc gggctcacgg tattcgtgcc gctgaatctc gcggctttcg cggttctgcc
     1261 ggagcgcggc acgttcagcc gccaaggcgc gctgcggctg ggagtgatcg gattacaggc
     1321 cgttctcgtc gtctgggccg cgacggcggg cgaggtcggg gttgtcgact gggcttacga
     1381 gcggtttctc gatcccgcgc cggtcagcgc cggacgcatc ccgcagctcg gcatcgcgct
     1441 gatcagcctg ggcctgttgc tcacgatggc tgcggcgctg gtgacgcgct ccgctactcc
     1501 cgcggggctg gcgggcgcga tcgtcgcctt cgcgatcgcc gcgcacgtcc ccacggcctc
     1561 cctcaccttc tcggtcttca ccgccatggc ggagctgatg gtgacgatcg ccgtgctgca
     1621 ggacaccttc cgcatggcgt tccgcgacga gttgacgggg ctgcccagcc ggcgcgcgct
     1681 caacgagcgg ctggacacgc tcggcaagag ctacgccatc gcgatggtcg acgtggacca
     1741 cttcaagaag ttcaacgaca cctacggcca cgacctcggc gaccaggtgc tgaagatggt
     1801 ggcggcgcac atctccggcg tgggcggcgg cgggaaggcc tttcgatacg gcggggagga
     1861 gttcacgatc ctgttccccg gcaaggagac agccgaggcg atcccgtacc tggaggcact
     1921 gcgcggggag atcgaatcct accgcctcgc gctgcgcggc tcggaccgtc cgaagaagac
     1981 gaagggcgac aagcgccagc gcggcgggtg gcgcggcaag gactcggtat ccgtaaccgt
     2041 gagcgtcggc gtggcggagc gcaacgaccg cctcgtcacc ccgcaggcgg tgatcgaggc
     2101 ggcggatcgt gccctctacc gcgccaagga aaaggggcgc aaccgggtga gccgatagac
     2161 ggccgccgca cgcggccgtt tataatccgc gtgccggttg aagcgcggcg cgccggaatt
     2221 cccgaaaagc ctgttcgctg cctgtgaacg tcccactgtc gaagcaacct tggttcgatg
     2281 ccgcgcttgc ctggtacttc gtcaccgtct ggggctcggg cttcatcgcc accaagatcg
     2341 gattgcaata cgccccgccc ttcacctttc tcacgctgcg tttcgcgttc ggcttggcgt
     2401 gcctcgtgcc gatcctgctc atcatgcggc cgcgcctccc ccgttcgcgc gccgagtttg
     2461 gccacgtcgt catcgcggga ctgctgatgc acgccgtcca tctcggcggc agccactaca
     2521 cgcagtatct cggcatgtcg gccggcatca ccgcgttgat gctttcggcg caaccgctca
     2581 tcacggcgct catcgccgcg cgctggctgg gcgagcggcc cgcggcccgc cagtgggcgg
     2641 gcgtgatgat cggcctcgcg ggcgtcgcgc tcatcgtgtg gcacaaggtg gacgtgcgcg
     2701 aagccaccct cggcagcctc gtcgccgtca cggtctcgct cacgggcgtc acggccggca
     2761 cgctctacca gcgcatctac tgcccgctgg tcgacctgcg cagcgcggcc ttcatccagt
     2821 tcatcgcgac catcgccgcg ctcgccccgc tcgcgtggct cgccgagggc ttcaccgtga
     2881 cgtgggcctg gccgatgctg ggctccattg ctttcctcgt gatcggggcc tcgatcctcg
     2941 cggtgagcgc gctgcatacg ctgatgcggc gcggccaggc cacccgcgtc accagcctcc
     3001 tctatctcac gccgatcttc gcggtggtgc tggagctggc gatgttcgga gtcgtgccga
     3061 gcgcgctttc ggtcgccggc atcgccgtca cctgctgcgg cgtcgcgct
//
LOCUS       DBNGWZ010000675         4005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1762600, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000675 DBNGWZ010000000
VERSION     DBNGWZ010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1762600"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC16CX_21040"
     CDS             complement(<1..234)
                     /locus_tag="AC16CX_21040"
                     /inference="COORDINATES: protein motif:HMM:NF025046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HIR3015028.1"
                     /translation="MRSIADALQSIAVTLWVGGLWAIGFIVAPLLFARVLDRALAGLV
                     AGKLFTLIAFIGIACALYLILFRLARFGAGCLKQ"
     gene            complement(320..796)
                     /gene="greA"
                     /locus_tag="AC16CX_21045"
     CDS             complement(320..796)
                     /gene="greA"
                     /locus_tag="AC16CX_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR3015029.1"
                     /translation="MGKIPITIAGAEKLRAELQELKTVQRPVIVNAIAEARSHGDIAE
                     NAEFHAAKERQGFIEGRIAELEAKLANAQIINPALLDADGRCVFGATVDLEDMASGEV
                     VTYQIVGDDEADLKLGMISISSPIARALIGKYPGDVAEVQAPGGVREYEILDVKYV"
     gene            complement(801..>4005)
                     /gene="carB"
                     /locus_tag="AC16CX_21050"
     CDS             complement(801..>4005)
                     /gene="carB"
                     /locus_tag="AC16CX_21050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3015030.1"
                     /translation="DIKSILIIGAGPIVIGQACEFDYSGAQACKALKEEGYRVILVNS
                     NPATIMTDPEMADATYIEPINWQMLEKIIAAERPDALLPTMGGQTGLNCALDLARNGV
                     LEKYGVEMIGAKREAIDKAEDREKFKKAMERIGLACPRAALAHSMEEALQVQAAVGFP
                     TIIRPSFTLGGTGGGIAYHKDEFLAIVERGLDASPTREVLIEESVIGWKEFETEVVRD
                     RKDNCIIVCSIENVDPMGVHTGDSITVAPAQTLTDKEWQIMRDASIACLREIGVDTGG
                     SNVQFAVNPGDGRMLIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELRNEI
                     TGGATPASFEPTIDYVVTKVPRFAFEKFPQADDRLTTQMKSVGEVMAIGRTFQESMQK
                     ALRGLETGSDGFDEKTTERDAIENELGDPGPDRIYYVADAFRCGMTVDEVHDLTRIDP
                     WFLAQIEDIVHQEKALEGGTLESLDADALRRLKRAGFSDRRLAKILGAEQAAVRAQRH
                     ALGIRPVFKRVDTCAAEFATYTAYLYSTYEEECEARPSDRRKIMVLGGGPNRIGQGIE
                     FDYCCVHAAFALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLEIVHVEQP
                     HGVIVQFGGQTPLKLARDLEANGVPIIGTSPDSIDIAEDRERFQKLLNRLKLRQPPNR
                     TARSTQKALAVAEEIGYPLVVRPSYVLGGRAMEIVYQQSDLERYMREAVKVSNDSPVL
                     LDHFLNDATEVDVDAVCDGKQAVIGGVMEHIEQAGVHSGDSACSLPPYSLSREIQEEL
                     KRQTIALAHALKVVGLINVQFAIQDGVVYVLEVNPRASRTVPFVSKATGVPLAKIAAR
                     CMAGKLLADQGVVGEIVPPYYSVKEAVFPFIKFPGADTILGPEMKSTGEVMGVGETFA
                     EAFVKSQLASGEDLPASGKVFVSVRDEDKARTVEIARKLASLGFALVATRGTAAALAA
                     GGLSVTPVNKVQEGRPHIVDMIKNGEISLIVNTVEEKRVAVRDSYAIRRAALQERVPL
                     YTTLDGARAACTGMEHAKELRAYVMQALHASLAETAH"
BASE COUNT          618 a         1382 c         1269 g          736 t
ORIGIN      
        1 ctgtttgaga caccccgctc cgaatcgcgc gaggcgaaag aggatgaggt agagcgcgca
       61 ggcgataccg atgaaggcga tgagggtgaa gagctttccg gccacgaggc cggcgagcgc
      121 ccggtccagc acccgcgcga acagcagcgg cgcgacgatg aagccgatcg cccaaaggcc
      181 acccacccaa agcgtgaccg cgatcgattg cagggcgtca gcgatgcttc gcatcgggta
      241 acgggttatg agtaatgggt aatgggctgc gaaaagtgac ccggatttct ctcatcaccc
      301 atcactcatc acccatcacc tagacgtact tgacgtcgag gatctcgtac tcccgcaccc
      361 ccccgggggc ctgcacctcg gcgacgtcgc ccgggtactt gccgatcagc gcgcgcgcga
      421 tcggcgagct gatcgagatc attcccagct tgaggtctgc ctcgtcgtcg ccgacgatct
      481 ggtaggtcac gacctccccg ctcgccatgt cctcgaggtc cacggtagcg ccgaacacgc
      541 agcgcccgtc ggcgtcgagc agggccgggt tgatgatctg ggcattggcg agcttcgcct
      601 ccagctcggc gatgcggccc tcgatgaaac cctggcgctc cttcgccgcg tggaattccg
      661 cgttctcggc gatgtcgccg tgcgaacgcg cctcggcgat cgcattgacg atcacaggcc
      721 gttgcacggt cttcagttcc tgcagctcgg cgcgcagctt ctccgcgccg gcgatcgtga
      781 tcgggatctt gcccatggtt tcagtgtgcc gtctcggcca ggctcgcgtg cagggcctgc
      841 atcacgtacg cgcgcagttc cttggcgtgt tccattccgg tgcacgcggc gcgcgcgccg
      901 tcgagcgtcg tgtacagcgg aacgcgttcc tgcagcgcgg cgcggcggat cgcgtaggag
      961 tcgcgcaccg ccacgcgctt ctcctccacc gtgttgacga tgagcgagat ctcgccgttc
     1021 ttgatcatgt ccacgatgtg cggccggcct tcctgaacct tgttcaccgg cgtgacgctg
     1081 agaccccccg ccgcgagggc cgccgcggtg ccgcgggtgg cgaccagcgc gaatccgagg
     1141 ctcgccagct tgcgcgcgat ctccacggtc ctcgccttgt cctcgtcgcg cacgctgacg
     1201 aacaccttgc cgctcgccgg caggtcctca cccgaggcga gttgcgactt gacgaaggcc
     1261 tctgcgaaag tctcgcccac gcccatgacc tcgccggtgg atttcatctc cggacccagt
     1321 atggtatcgg cgccggggaa cttgatgaac ggaaacaccg cttccttcac cgagtagtac
     1381 ggcggcacga tttctcccac cacgccctgg tcggcgagga gtttccccgc catgcagcgc
     1441 gccgcgatct tggcaagcgg cacgcccgtc gccttggaaa cgaacggcac cgtgcgcgag
     1501 gcgcgtggat tgacctccag cacgtacacc accccgtcct ggatcgcgaa ctgcacgttg
     1561 atcagtccca cgaccttgag cgcatgggcc aacgcgatgg tctggcgctt gagctcttcc
     1621 tggatctccc gggacagcga atacggcggc agcgagcagg cggaatcgcc ggaatgcacg
     1681 ccggcctgct cgatgtgctc catcacgccg ccgatcaccg cctgtttgcc gtcgcacacc
     1741 gcgtccacgt ccacttcggt ggcgtcgttg aggaagtggt cgagcagcac cggcgagtcg
     1801 ttcgacacct tgaccgcctc gcgcatgtag cgttcgaggt cgctctgctg atagacgatc
     1861 tccatcgcgc ggccgcccag gacgtaggaa ggccgcacca ccagcgggta gccgatctcc
     1921 tccgccaccg cgagcgcttt ctgcgtgctg cgcgcggtcc ggttgggcgg ctgcctgagc
     1981 ttgagcctgt tcagcagctt ctggaaacgc tcgcggtctt ccgcaatatc gatcgagtcg
     2041 ggcgaagtgc cgatgatggg cacgccgttc gcttcgagat cgcgcgcgag cttgagcggt
     2101 gtctgcccgc cgaactgaac gatcacgcca tgcggctgct cgacgtggac gatttccagc
     2161 acgtcctcga gtgtgagcgg ctcgaaataa agccggtcag aggtgtcgta gtcggtcgag
     2221 acagtctccg ggttgcagtt gaccatgatg gtctcgaacc cgtcctcgcg cagcgcgaac
     2281 gccgcgtgca cgcagcagta gtcgaactcg atgccctggc cgatgcggtt gggtccaccg
     2341 cccagcacca tgatcttcct gcggtcgctc ggccgcgcct cgcactcctc ctcgtaggtc
     2401 gagtagaggt aggcggtata ggtcgcgaac tccgcggcgc acgtgtccac ccgcttgaaa
     2461 accgggcgga tgccgagcgc atggcgctgc gcgcgcaccg cggcctgttc ggcgccgagg
     2521 atcttcgcaa gccgccggtc ggagaatccc gcgcgcttga gccgccgcag cgcgtccgca
     2581 tcgaggctct cgagcgtccc gccctcgagc gccttctcct ggtgcacgat gtcctcgatc
     2641 tgcgcgagga accagggatc gatgcgcgtc aggtcgtgca cctcgtccac cgtcatgccg
     2701 cagcggaagg cgtcggcgac gtagtagatg cggtcggggc cggggtcgcc cagctcgttc
     2761 tcgatcgcgt cgcgctcggt ggtcttctcg tcgaatccgt ccgatccggt ctcgagcccc
     2821 cgcaatgcct tctgcatgga ctcctggaag gtgcggccga tcgccatcac ctcgcccacc
     2881 gacttcatct gcgtcgtcag gcggtcgtcc gcctgcggaa atttctcgaa cgcgaagcgc
     2941 ggcaccttgg tgacgacgta gtcgatggtc ggctcgaacg aggcgggcgt cgccccgccg
     3001 gtgatttcgt tcctcagctc atcgagcgta tagcccaccg cgagcttggc cgcaaccttg
     3061 gcgatcggaa aaccggtcgc cttcgatgcc agggcggagg agcgcgacac ccgtggattc
     3121 atctcgatga tcagcatgcg cccgtccccg ggattcaccg cgaactgcac gttggagccg
     3181 ccggtgtcca cgccgatctc gcgcaggcac gcgatcgacg cgtcgcgcat gatctgccac
     3241 tccttgtcgg tgagggtctg cgcgggcgcg accgtgatcg agtcgccggt gtgcacgccc
     3301 atcgggtcca cgttctcgat cgagcacacg atgatgcagt tgtccttccg gtcgcgcacc
     3361 acctccgtct cgaattcctt ccagccgatc accgactcct cgatcagcac ctcgcgcgtg
     3421 gggctggcgt cgagcccgcg ctcgacgatg gcgaggaact cgtccttgtg gtaggcgatg
     3481 ccgccgccgg tgccgccgag cgtgaacgaa ggccggatga tggtcgggaa gccgaccgcc
     3541 gcctgcacct gcagcgcctc ctccatgctg tgcgcgagcg ccgcgcgcgg gcaggcgagc
     3601 ccgatccgct ccatcgcctt cttgaacttc tcgcggtcct cggccttgtc gatcgcctcg
     3661 cgcttcgcgc cgatcatctc gacgccgtac ttctcgagca ccccgttgcg cgcgaggtcg
     3721 agcgcgcagt tcaagcccgt ctgcccgccc atcgtcggca gcagcgcgtc cgggcgctcg
     3781 gctgcgatga tcttttccag catctgccag ttgatcggct cgatgtaggt cgcgtccgcc
     3841 atctccgggt cggtcatgat ggtggcgggg ttggaattca ccaggatgac gcgatagccc
     3901 tcctccttga gcgccttgca cgcctgcgcc ccggaatagt cgaactcgca cgcctggccg
     3961 atcacgatcg gcccggcgcc gatgatcagg atgctcttga tgtcg
//
LOCUS       DBNGWZ010000676         3737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_3273695, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000676 DBNGWZ010000000
VERSION     DBNGWZ010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_3273695"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16CX_21055"
     CDS             complement(<1..199)
                     /locus_tag="AC16CX_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3015031.1"
                     /translation="MPSAAATNEATPTLASVIVLKIQEFARRPVAEQSGLKAQLEALV
                     ALAIRPLPAANRVVLDTSDGVT"
     gene            complement(256..1920)
                     /locus_tag="AC16CX_21060"
     CDS             complement(256..1920)
                     /locus_tag="AC16CX_21060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR3015032.1"
                     /translation="MTKLENISARPVASDVLPRGFALLHDPTLNKGTAFTERERDALG
                     VRGLLPPHVLSQDEQAARVLKYIRQLPNDLEKYIALIALHDRNETLFFRIVCDHIDEI
                     QPIIYTPTVGLACQKYGHIFQRPRGLFIGSNDRGRIAEVLQNWPYPAGLIVVTDGERI
                     LGLGDLGANGMGIPVGKLSLYVACAGIHPSLCLPVVLDFGTNNDELLSDPYYVGLRQR
                     RVRGAAYDELVEEFVTAARQVFPGVVIQFEDFATQNAFRLLARYRDRTPAFNDDIQGT
                     AAVALAGMFSALRVTGGELTGQKILFLGAGEAATGIADLVVSAMTAQGLTEQGARHRC
                     WLVDSRGLVVKSRAALAEHKLPYAHDHAPIGDFLAAIKALKPTAIVGVAAVGGTFTRE
                     VLEEMARINERPIVFALSNPTSKSECTAEEAYRHTGGRALFACGSPFDPVTIDGTTFV
                     PRQGNNSYIFPGVGLGAIASRARKITDEMFMGAAHALAHCVTESDLKQGSLYPALPRI
                     REVSASIAAKVAQIAYRRGLAARPVPADIEADVKSKMYEAVYQSCV"
     gene            complement(2128..3120)
                     /locus_tag="AC16CX_21065"
     CDS             complement(2128..3120)
                     /locus_tag="AC16CX_21065"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HIR3015033.1"
                     /translation="MNLQLSIGMSSNPRTWPIFDGRVKPDGIDLIPSRVHPSELFWRQ
                     LKFVDFDVSEMSFSSLMMATSRGDDRWVGVPVFTTRKFYHAAILVRRKAGIASPADLK
                     GKRVGVPEYQQTAALWARGVLQDEFGVEPRHMEFWMERGASHSHAGAVGFTAPPGVTI
                     HTVPPEKNLGAMMLAGELDALLHYIVDANLVDRSTADLWNHPDVGPLFPDPWAEGIRY
                     YRKTGIFPINHGMVIRREIAEKHPWAVLNLLKAFDRANDIANRERVEHAEYHVAAGLL
                     PSEAGEALRTPLVRHGIHANRKTLETAARYSREQGLTPRVIKLEEVFAQSAMEQ"
     gene            3502..>3737
                     /locus_tag="AC16CX_21070"
     CDS             3502..>3737
                     /locus_tag="AC16CX_21070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3015034.1"
                     /translation="MPNRSIRAIIQEQELVTAAENTTVSEAARLMRDKQVGAVMVVEG
                     DKLAGIFTERDALFRIVAEGRDPRATRLADVMTR"
BASE COUNT          626 a         1190 c         1281 g          640 t
ORIGIN      
        1 ccgtgacccc gtccgaggta tcgaggacga cgcggttggc cgcgggcagg gggcgaatcg
       61 cgagcgcaac gagcgcctcg agctgcgcct tgagcccgct ctgctccgcc accggccggc
      121 gggcgaactc ctggatcttc aggacgatca cgctcgcgag cgtgggcgtg gcttcgttcg
      181 ttgctgcggc ggacggcatg gtgccgtcat tttaaccgcg ccgccggggg aacgtgtgac
      241 cgatgaccgg cggcgtcaga cgcagctctg atagaccgcc tcgtacatct tggatttgac
      301 gtccgcctca atatccgccg gcaccggtct cgcggcgagg ccgcgccggt aggcgatctg
      361 cgccaccttg gccgcgatgc tggccgagac ctcgcggatg cgcggcagcg cggggtagag
      421 gcttccctgc ttgaggtccg actccgtgac gcaatgcgcg agcgcgtggg ctgcgcccat
      481 gaacatctcg tccgtgatct tgcgcgcgcg gctggcgatc gcgcccagcc ccacgcccgg
      541 gaagatgtag gagttgttgc cctgccgcgg cacgaaggtc gtgccgtcga tcgtgaccgg
      601 gtcgaacggg ctgccgcagg cgaagagcgc ccgtccgccg gtgtggcgat aggcctcctc
      661 ggcggtgcac tcggacttgg acgtcgggtt ggaaagcgca aatacgatcg gccgctcgtt
      721 gatccgggcc atttcctcga gcacttcgcg cgtaaaagta ccgccgaccg cagcgacgcc
      781 gacgatggcc gtcggcttga gcgccttgat cgcggcgaga aagtccccga tcggcgcgtg
      841 atcgtgcgcg tacggcagct tgtgttctgc aagggcggcg cggctcttca cgacgagccc
      901 gcgggaatcc accagccagc agcggtggcg cgccccctgc tcggtgagcc cctgcgccgt
      961 catcgccgag accaccaggt cggcgatgcc ggtcgccgcc tcgcccgcgc ccaggaacag
     1021 gattttctgg ccggtcaatt cgccgccggt gacgcggagc gccgagaaca tgccggcgag
     1081 cgcgaccgcc gccgtgccct ggatgtcgtc gttgaaggct ggggtgcggt cgcggtagcg
     1141 ggcgagcagc cggaacgcgt tctgagtggc gaaatcctcg aactggatca ccaccccggg
     1201 gaatacctga cgggcggcgg tgacgaactc ttccaccagc tcgtcgtagg cggccccgcg
     1261 cacgcggcgc tggcgcagcc cgacgtagta ggggtcggag agcagctcgt cgttgttggt
     1321 gccgaagtcg agcaccaccg gcaggcacag gctgggatga atcccggcgc aggcgacata
     1381 gagcgagagc ttgcccacgg ggatgcccat gccgttcgcg ccgaggtcgc cgagtccgag
     1441 tatgcgctcg ccatcggtca ccacgatgag gccggcgggg tacggccagt tctgcagcac
     1501 ctcggcgatg cgcccgcggt cgttggaccc gatgaacagc cccctcggcc gctggaagat
     1561 atgcccgtac ttctggcagg cgagccccac cgtcggcgtg tagatgatcg gctggatctc
     1621 gtcgatgtgg tcgcacacga tgcggaagaa cagcgtctcg ttgcggtcgt gcagcgcgat
     1681 cagcgcgatg tacttctcga gatcgttggg gagctgccgg atgtacttga gcacgcgcgc
     1741 ggcctgctcg tcctgggaaa gcacgtgcgg cggcagcaga ccgcgcacgc ccagggcgtc
     1801 gcgctcgcgc tcggtgaacg cggtgccctt gttgagcgtc gggtcgtgca ggagcgcgaa
     1861 cccccgcggc aggacatcgc ttgcaaccgg acgcgcgctg atgttctcca gcttcgtcat
     1921 ggcggtgctc ctccttcgcg ccgcggcgcg ttccggtcat tctccgccgc ccggggaggg
     1981 gggtattgac ggagatcaag tcgcgcgggg gtggcgatcc gggaaaggag atcgacgaaa
     2041 ccgcgagcgg gaaaccgaac cgtcggggaa cacaatgaaa acaaagctga atcagccgcc
     2101 ggcgtaaagc aatttctgcg ggatttctta ttgctccatc gcgctttgcg cgaacacttc
     2161 ctcgagcttg atcacccgcg gcgtcagtcc ctgctcgcgg gagtagcgcg cggcggtttc
     2221 cagcgtcttg cggttcgcgt ggatgccgtg ccgcaccagc ggcgtgcgca gcgcttcccc
     2281 ggcctcgctc ggcagcaggc cagcggcgac gtgatattcc gcgtgctcca cgcgctcgcg
     2341 gttcgcgatg tcgtttgcgc gatcgaacgc cttgaggagg ttcaggaccg cccagggatg
     2401 cttctcggcg atttcccgcc tgatcaccat gccgtgattg atcgggaaga tgccggtctt
     2461 gcggtagtag cggatgcctt cggcccacgg atccgggaaa agcggaccga cgtcaggatg
     2521 gttccacagg tcggcggtgc tgcggtcaac caggttggca tccacgatgt aatggagcag
     2581 cgcatcgagc tcgcccgcga gcatcatggc accgaggttc ttctcgggcg ggaccgtgtg
     2641 gatcgtcact ccgggtgggg cggtgaagcc cacggcgcct gcatggctgt gcgacgcgcc
     2701 tcgctccatc cagaattcca tgtgccgcgg ctcgacgccg aattcgtcct gaagcacgcc
     2761 gcgcgcccat aacgccgcgg tctgctggta ttccggcacg ccgacccgct tgcccttgag
     2821 gtcggcgggg gacgcgattc ccgccttgcg ccgcaccagg atcgcggcat ggtagaactt
     2881 cctggtggtg aaaaccggca cgccgaccca gcggtcgtcg ccacgggatg tcgccatcat
     2941 cagtgaggaa aacgacattt ccgagacgtc gaaatccacg aacttgagct ggcgccagaa
     3001 caactccgaa ggatgcaccc ggctcggaat caggtcgatg ccgtcgggct tcacccggcc
     3061 gtcgaagatc ggccaggtcc tcggattcga ggacatgccg atgctgagct ggaggttcat
     3121 gacgaggagt ttgtcgggat tggatcgaac aaactcctga tgcaaaacca cctccacgaa
     3181 aaagcgtagt aagggcaata cgagcctgat ttcggtgcca cttgcatggc ccgtgttagt
     3241 ctgtcagcaa gcagcactct cagtttcatt ccaaatcggg aggtggtttt gctctcagca
     3301 gcctgtcgga agcaaaggcc gacaggctgc tggagagtat ggcaaacggt ggcaggatca
     3361 acaagccatc ctcgctggtt tttgaccgct ccgcgcggca ggcgtagcat gttctcaagc
     3421 cgcgacgcgc ggcttcgatt tgggtccgcg agccgccggc aacccggcgc cggagtccgt
     3481 atgaacgatc gggggtgtgc catgccgaac cgttccatca gggcgatcat ccaggaacag
     3541 gagctggtca cggctgccga gaacaccacg gtcagcgagg ccgcccgcct catgcgcgat
     3601 aagcaggtcg gagccgtgat ggtggtggag ggggacaagc tggccgggat tttcaccgag
     3661 cgcgacgcgc tgtttcgcat cgtcgcggag ggccgcgatc cgcgcgccac ccggcttgcc
     3721 gacgtcatga cccgcaa
//
LOCUS       DBNGWZ010000677         3037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1254890, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000677 DBNGWZ010000000
VERSION     DBNGWZ010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1254890"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16CX_21075"
     CDS             complement(<1..205)
                     /locus_tag="AC16CX_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR3015035.1"
                     /translation="MSAAKPTMNSESNRDYPLSGITVIDLSHVYNGPYATFLMAMAGA
                     NVIKVEPLDGEHLRSRGDLGGAAL"
     gene            complement(262..1128)
                     /locus_tag="AC16CX_21080"
     CDS             complement(262..1128)
                     /locus_tag="AC16CX_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3015036.1"
                     /translation="MRSLLFVPGDDERKIAKGLGSAADALILDLEDAVAPARKGAARE
                     ICAKALQSANTTKKLFVRVNALDTGQTLADLAAVVCGRPYGIVLPKCRGGDDARLVGH
                     YLSVLEAREGIESGATRILPIATENGASMFGLGSYAGVERLCGIFWGGEDLAADLGAV
                     ANRDTHGRYTAPYELARSLTLFAAAAAQVDAVDAVYTNFRDLEGLRAEAAEALRDGFS
                     AKAAIHPDQIEPINAAFTPSAADVAWAKRIVAAFDESPGAGVASLDGKMLDRPHYRSA
                     QRVLARAEQAQS"
     gene            complement(1148..2311)
                     /locus_tag="AC16CX_21085"
     CDS             complement(1148..2311)
                     /locus_tag="AC16CX_21085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3015037.1"
                     /translation="MNFELTQEQRSMVNTVRELTQEKFKPNALKYMDGTFPWDNIRDL
                     AKIGVLGMAVPEEYGGLELPVLDTALVIEEISKGCYVTAMAVMGEVGVQARIISTYAP
                     EHIKRAILPKVCTGEALLAVCMTEPHAGTDVANYRTNADVVGDKIVLNGVKTLISRVD
                     EAQAFVVFSRVNKQPGREGIGCVLIERGTPGFTCTARYHTMGGENLAEIQFQNCELPL
                     ENLIIREDGFRKLLSAFNTQRCLNPSVSLGLAEGAFEEAVKYARERTAFGRPIGEFQA
                     IRHKLAEMYRDIEASRSLLYRACATANPFPDPYMAAIAKITVNEMSLRVTSDAIQVHG
                     GYGFTDEFPVSRFYRGARYGTLGGGTTETLKELVGKMILSDFGPDGFLDVGGF"
     gene            complement(2328..>3037)
                     /locus_tag="AC16CX_21090"
     CDS             complement(2328..>3037)
                     /locus_tag="AC16CX_21090"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3015038.1"
                     /translation="LLERIIAAKLKPGSSFATEAELLSQFNVSRPTLRESLKLLESQG
                     LLDRRPGPGGGIIVREPSTDLLAHGLSVFLRLHEVPFVTVLKAREVIEPALAYEAAVN
                     GSEEDFGELEASIVRMKALDAQSDQEAFLEENRVFHSVIARASGNKVLEVFWETISIL
                     ATGEHHGIRYSVGNQAHVVKAHQRILDACRRRDGNAAAAAMESHVTALENLVRARYQH
                     LLKHPTSVVERPGRSIA"
BASE COUNT          486 a          996 c          986 g          569 t
ORIGIN      
        1 gaagggcagc ccctccgaga tcgccgcggc tgcgcagatg ctcgccatcg agcggctcga
       61 ccttgatcac gttcgcgccc gccatcgcca tgagaaacgt ggcgtagggc ccgttgtaga
      121 cgtgcgaaag atcgatgacc gtgatacccg aaagcgggta atcgcgattc gactcgctgt
      181 tcattgtcgg ttttgctgcg gacatgctgc ctccggaatt caaatcgtga ctggttgttc
      241 gtgatgcggc gcgtttcgcg atcagctctg cgcctgctcc gctcgcgcga gcacgcgctg
      301 cgcgctgcgg taatgcgggc ggtcgagcat tttgccgtcg agcgacgcga cgcccgcacc
      361 gggcgattcg tcgaacgccg cgacgatgcg cttcgcccat gcgacatcgg cggcggatgg
      421 cgtgaaggcc gcgttgatcg gctcgatctg atcgggatgg atcgcggcct tcgccgagaa
      481 gccgtcgcgg agcgcttccg ccgcctcggc cctgaggcct tcgagatcgc ggaagttcgt
      541 atagaccgca tcgacggcat cgacctgcgc cgccgctgcg gcgaacagcg tcagcgaacg
      601 cgcgagctcg tacggcgcgg tatagcggcc atgcgtgtcg cggttcgcga cggcgcccag
      661 atcggctgcg aggtcctcgc cgccccagaa aatgccgcag agccgctcga ccccggcgta
      721 gctacccaga ccgaacatgg acgcaccgtt ctcggtcgcg atcggcagga tgcgcgtggc
      781 gccgctctcg atgccctcac gtgcttcaag gacggaaagg tagtggccga cgagcctcgc
      841 gtcgtcgccc ccgcggcact tcggcagcac gatcccgtac ggccgcccgc acaccaccgc
      901 cgcgagatcc gcaagtgtct gacccgtgtc gagcgcgttc acgcgcacaa agagtttttt
      961 cgtcgtgttc gccgattgca gcgctttcgc gcagatctcg cgcgcagcgc ctttgcgcgc
     1021 cggcgcaacc gcatcttcga gatcgaggat cagcgcgtcg gccgcggagc cgaggccttt
     1081 ggcgatcttg cgttcgtcgt cgcctggaac gaaaagcagg gagcgcatag ggctgatcga
     1141 gcgcggctca gaagcccccc acgtcaagaa aaccgtcggg gccgaagtcg gacaggatca
     1201 tcttgccgac cagctccttc agcgtttcgg tcgtgccgcc gcccagcgtg ccgtagcgcg
     1261 caccgcgata gaagcgcgag accggaaact cgtcggtgaa gccgtaaccg ccgtgcacct
     1321 ggatcgcatc gctggtcacg cgcagcgaca tctcgttcac ggtgatcttc gcgatcgccg
     1381 ccatgtaagg atccgggaac ggattcgccg tcgcgcacgc gcgatagaga agcgagcggc
     1441 tcgcttcgat gtcgcgatac atctcggcaa gcttgtgccg gatcgcctgg aactcgccga
     1501 tcgggcggcc gaacgcggta cgctcgcgcg cgtacttcac cgcctcctcg aatgcgcctt
     1561 ccgcgaggcc gagcgagacg ctcggattga gacaccgttg cgtattgaac gcgctcagga
     1621 gcttgcggaa accgtcttca cggatgatca ggttctccag cggcagctcg cagttctgga
     1681 actggatctc ggcgaggttc tcgccgccca tcgtgtgata gcgcgcggtg caggtgaatc
     1741 ctggagtgcc gcgctcgatc aggacgcagc cgatcccttc gcggcctggc tgtttgttca
     1801 cgcggctgaa gacgacgaac gcctgcgcct cgtccacccg gctgatcagg gtcttcaccc
     1861 cgttcagcac gatcttgtcg ccgaccacgt ccgcgttcgt gcggtagttc gcgacgtcgg
     1921 tgccggcgtg aggctcggtc atgcacaccg cgaggagcgc ttcgcccgtg cacaccttgg
     1981 gcaggatcgc gcgctttatg tgctctggcg cgtacgtgga tatgatgcgc gcctgcacac
     2041 ccacttcgcc catcaccgcc atcgcggtta cgtagcagcc tttcgagatc tcttcgatta
     2101 cgagcgccgt gtccagcacc ggaagctcga gcccgccata ctcctcgggc accgccatgc
     2161 cgaggacgcc gattttggcg agatcgcgga tattgtccca cgggaacgtg ccgtccatgt
     2221 acttcagggc gttcggcttg aatttttcct gcgtgagctc gcgcacggtg ttcaccatgc
     2281 tgcgttgttc ttgcgtcagt tcgaagttca tagaggctcc tgtcgggtta cgcgatcgag
     2341 cggccgggcc gctcgacgac gctggtcggg tgcttcaaga gatgctggta gcgcgcgcga
     2401 acgagatttt cgagggcggt cacgtggctt tccatcgcgg cggcggcggc gttgccgtcg
     2461 cgccggcggc atgcgtcgag gatgcgctga tgcgccttca cgacgtgcgc ctggttgccg
     2521 accgaatagc ggatgccgtg atgctctccg gtcgcgagga tgctgatcgt ctcccagaaa
     2581 acctcgagca ccttgttgcc gctcgcgcgc gcgatgacgc tgtggaagac gcggttttct
     2641 tcgaggaacg cctcctggtc gctctgcgcg tcgagcgctt tcatgcgcac gatcgacgct
     2701 tcgagctccc cgaagtcctc ttcgcttccg ttcacggccg cctcgtatgc gagcgcgggc
     2761 tcgatcactt cgcgcgcctt gagcaccgtc acgaacggca cttcgtgcag gcgcaggaac
     2821 accgagagcc cgtgcgccaa cagatcggtg ctcggctcgc gcacgatgat gccgccgccg
     2881 gggccggggc gacgatcgag caggccctgg gattcgagaa gcttgaggct ctcgcgcagc
     2941 gtcggccggc tcacattgaa ctgcgagagg agctcggctt cggtcgcgaa gctgctgccg
     3001 ggcttcaact tcgcggcaat gatgcgctcg agcagct
//
LOCUS       DBNGWZ010000678         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1133452, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000678 DBNGWZ010000000
VERSION     DBNGWZ010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1133452"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="tcuA"
                     /locus_tag="AC16CX_21095"
     CDS             <1..1353
                     /gene="tcuA"
                     /locus_tag="AC16CX_21095"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR3015039.1"
                     /translation="TAGLMRVVYNGVDDLAQLIPDLTDEEKKTHDFGSYTAEHYYDDM
                     GRITQYRTDPDLCEILISRSFETLVWLRKKGVRFQASYGRQSYKVEGNRYKFWGGLAA
                     ETWGGGPGLIDNEHKACERAGIRIFYETPAIGLVTNEDGVVLGVRAKHKGKTVEIRSK
                     AVVLACGGFESNAEMRARYLGPGWDLAKVRGTRYNTGLGIRMALEAGAMPYGHWSGCH
                     AVGWDVNAPPFGDLAVGDQFQKHSYPWGIMVNARGERFVDEGADFRNYTYAKYGRVIL
                     EQPGMFAWQIFDAKIIPMLRDEYRIKQVTKARADTLEELVKKLEGVDAANCLREIREY
                     NKAVRIDVPFNMAVKDGRCTVGLRINKSNWANTIDQPPFEAYGVTCGVTFSFGGVKIG
                     KQGEVEDTAGKPIPGLYAAGELVGGIFYHNYPGGTGLTSGAVFGRLAGTGSAAYVKRK
                     "
     gene            1424..>1787
                     /locus_tag="AC16CX_21100"
     CDS             1424..>1787
                     /locus_tag="AC16CX_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3015040.1"
                     /translation="MGPYRAPRWLPGGHAQTVYAALLAPRPRIAYRRERWDTPDGDFI
                     DVDWTVEPSAACRQRSAAADGAPLVVLFHGLEGSSRSHYALALMAEVALRGWRGAVVH
                     FRGCSGELNRLPRAYHSGD"
BASE COUNT          331 a          575 c          596 g          285 t
ORIGIN      
        1 acggcgggcc tcatgcgcgt ggtctataac ggcgtggacg acctcgcgca gctcattccc
       61 gacctcaccg acgaggagaa gaagacccac gacttcgggt cctacaccgc cgagcattac
      121 tacgacgaca tgggccgcat tacccagtac cgcaccgatc ccgacctgtg cgagatcctg
      181 atctcgcgca gcttcgaaac cctcgtatgg ctgcgcaaga agggcgtccg gttccaggca
      241 agctacgggc gccagtcgta caaggtcgag ggcaaccgct acaagttctg gggcgggctc
      301 gcggccgaga cctggggcgg cgggccggga ctgatcgaca acgagcacaa ggcgtgcgag
      361 cgcgcaggca tccggatctt ctacgagacg ccggcgatcg gcctcgttac caacgaggac
      421 ggcgtggtgc tcggggtgcg tgcaaagcac aagggaaaga ccgtcgaaat ccgctcgaaa
      481 gcggtggtgc tggcatgcgg cggcttcgag tccaacgccg agatgcgcgc gcgctacctc
      541 ggcccgggat gggatctcgc caaggtgcgc ggcacccgct acaacaccgg actcggcatc
      601 cgcatggcgc tggaagcggg cgccatgccc tacggccact ggtccggctg ccacgcggtg
      661 ggctgggacg tcaatgcgcc cccattcggc gatctcgcgg tcggcgacca gttccagaag
      721 cacagctatc cgtggggcat catggtgaac gcgcgcggcg agcgcttcgt ggacgaggga
      781 gcggatttcc gcaactatac ctacgccaag tacggccggg tcatcctcga gcagccgggc
      841 atgtttgcat ggcagatctt cgacgcgaag atcatcccca tgctgcgcga cgagtaccgc
      901 atcaagcagg tcaccaaggc gagggccgac acgctggagg agcttgtgaa gaagctcgaa
      961 ggggtggacg cggccaactg cctgcgcgag atccgcgagt acaacaaggc ggtgcgcatc
     1021 gacgtgccct tcaacatggc ggtcaaggac gggcgctgca ccgtcggctt gaggatcaac
     1081 aaatcgaact gggcgaacac catcgatcag ccgccgttcg aagcctacgg cgtcacctgc
     1141 ggcgtcacgt tcagcttcgg cggcgtgaag atcggcaaac agggcgaggt cgaagatacc
     1201 gccggcaagc ccatcccggg gctctacgct gcgggagaac tggtgggcgg catcttctac
     1261 cacaactacc cgggcggcac cggtctcacc tcaggcgccg tgttcggcag gcttgcggga
     1321 acgggttccg ccgcgtatgt gaagcggaaa taggaaataa tggggaatag cgcagcgcgc
     1381 ttaatagtcc attcaccatt cactattcat cattcacgcc gttgtgggtc cataccgcgc
     1441 gccacgctgg ctccccggcg gacacgcgca gaccgtttac gccgccctgc tcgctccgcg
     1501 cccgcgcatc gcctaccgcc gtgagcgctg ggacacgccg gacggcgatt tcatcgatgt
     1561 cgattggacc gtcgagccgt cagccgcctg ccgtcagcgg tctgcggcag ccgatggcgc
     1621 gccgctcgtg gtgctgtttc acgggctcga gggttcgtcc cggagccatt acgcgctcgc
     1681 gctcatggcg gaggtcgcgc tgcggggttg gcgcggggcg gtcgttcact ttcgcggctg
     1741 cagcggcgag ttgaaccgcc tgccgcgcgc ttaccactcg ggggaca
//
LOCUS       DBNGWZ010000679         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_2391504, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000679 DBNGWZ010000000
VERSION     DBNGWZ010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_2391504"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="AC16CX_21105"
     CDS             <1..1317
                     /locus_tag="AC16CX_21105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR3015041.1"
                     /translation="LIKNADTAMYRAKDVGRNSYQFYTPEMNARALEMLGMENSLRHA
                     LDRNEFLLYFQPKAGMADGDIVGVEALLRWQHPGRGLVSPGEFMPVLEETGLIVPVGE
                     WVLKAVCAQIKEWEPAGIKPVPVAVNLSARQFTERNLGPTIRRILEEYRVDPSLIELE
                     ITESSLMANTEEAVRTLEYLEELGVGLSIDDFGTGYSSFGYLKRFPLTALKIDRSFVQ
                     DITTDSDDATITRAVISMAHSLGLKVIAEGVETESQLAFLAEYGCDEIQGYYFSRPLA
                     VDDCGTWLRERRRLQRAPQALETAPVILLVDDDDDALTLLKRSLAKDGYRMLTARNAH
                     DALQHLGNERVDVVISDQNMPGIPGSEFLQRVKSLSPRTMRIMTSGFNDFQAVADAVN
                     KGEIFRFLPKNLGEEKLRSGVREALQARAESVASSGGYATLGVPGR"
     gene            complement(1340..1708)
                     /locus_tag="AC16CX_21110"
     CDS             complement(1340..1708)
                     /locus_tag="AC16CX_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003266231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR3015042.1"
                     /translation="MKLHLTANEDLQLFSGYGSGYFAVNKVRYEKCVVVTPQAVTEWA
                     VGGFEALTASDFGFIADLKPEVVIFGTGATQRFPRPELARALAATGVGLEVMDSRAAC
                     RTYNILASEGRKVVAAVLLD"
BASE COUNT          323 a          574 c          573 g          294 t
ORIGIN      
        1 ctgatcaaga acgcggacac ggcgatgtac cgcgccaagg atgtggggcg caacagctac
       61 cagttctaca cgcccgaaat gaacgcgcgg gcgctcgaga tgctgggcat ggaaaacagc
      121 ctgcgccacg cgctggaccg caacgagttc ctgctctatt tccagcccaa agccggcatg
      181 gccgacggcg acatcgtcgg agtggaagcc ctgttgcgct ggcagcatcc ggggcgcggc
      241 ctggtttcac ccggcgaatt catgccggtg ctggaggaga ccggcctgat cgtgccggtc
      301 ggcgagtggg tgctcaaggc cgtttgcgcc cagatcaagg aatgggagcc cgccggcatc
      361 aagccggtgc cggtcgccgt caacctgtcc gcgcggcaat tcacggaacg caacctcggg
      421 ccgaccatcc gccgcatcct ggaggagtat cgggtggatc cgtcgctgat cgagctcgag
      481 atcacggaaa gctccctcat ggccaacacc gaggaagccg tgcgcacgct cgagtacctc
      541 gaggagctcg gcgtgggcct gtcgatcgac gacttcggca cgggctattc gagtttcggc
      601 tatctcaagc ggttcccgct caccgccctg aagatagacc gctccttcgt gcaggacatc
      661 accaccgatt ccgatgacgc caccatcacc cgggcggtga tctcgatggc gcacagcctg
      721 ggcctgaagg tgatcgccga aggcgtggag accgagtcgc aactcgcctt tctcgccgag
      781 tacggctgcg acgagatcca gggctattat ttctcgcgcc cgctggcggt ggacgactgc
      841 ggcacctggc tgagggaaag gcggcgcctg cagcgcgcgc cgcaggccct cgagacggcg
      901 ccggtgatct tgctggtgga cgacgacgac gacgccctga ccctgctcaa gcgctcgctc
      961 gccaaggacg gctaccggat gctcaccgcc cgcaacgcgc acgacgcgct gcagcatctc
     1021 ggcaacgagc gcgtcgacgt ggtgatttcc gaccagaaca tgccgggaat cccgggcagc
     1081 gagttcctgc aacgtgtgaa atcgctgtcg ccgcggacga tgcgcatcat gaccagcggc
     1141 ttcaacgact ttcaggccgt ggccgacgcg gtcaacaagg gcgagatctt ccgctttttg
     1201 ccgaaaaacc tgggcgagga gaaactgcgc tccggcgtgc gggaggcatt gcaagcccgc
     1261 gcggagtcgg tggcgtcgag cggcggctac gctaccctgg gcgtgccggg caggtaatcc
     1321 ggcgggcgcc gcgcgccggt cagtcgagca gcacggccgc gacgaccttg cggccttcgg
     1381 acgcaaggat gttgtaggtg cggcaggcgg ccctgctgtc catcacttcc aggcccaccc
     1441 cggtggcggc gagcgcgcgc gcgagctccg gccgcgggaa gcgttgcgtt gcccccgtgc
     1501 cgaagatcac gacctcgggc ttcaagtccg caatgaatcc gaaatcagaa gcggtgagcg
     1561 cctcgaaacc gccgaccgcc cactccgtga ccgcctgcgg cgtcaccacc acgcatttct
     1621 catagcgcac tttgttgacg gcgaagtagc ctgagccgta cccggagaac aactgcaggt
     1681 cttcgttggc ggtcaagtgc agtttcataa cctactgatt tgcttcgggt tagttgctcc
     1741 gatagaatta tacgcttttg cgcc
//
LOCUS       DBNGWZ010000680         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate MAG05439
            SRR21617309_k127_1888724, whole genome shotgun sequence.
ACCESSION   DBNGWZ010000680 DBNGWZ010000000
VERSION     DBNGWZ010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414847
            Sequence Read Archive: SRR21617309
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
            Burkholderiales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 22:33:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,198
            CDSs (total)                      :: 4,155
            Genes (coding)                    :: 4,117
            CDSs (with protein)               :: 4,117
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 4 of 38
            Pseudo Genes (incomplete)         :: 31 of 38
            Pseudo Genes (internal stop)      :: 5 of 38
            Pseudo Genes (multiple problems)  :: 2 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617309_k127_1888724"
                     /isolate="MAG05439"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.68 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="AC16CX_21115"
     CDS             <1..1336
                     /locus_tag="AC16CX_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HIR3015043.1"
                     /translation="VILTGEALRRQNAEAIAGLLAENGGEFVCAAAGHHLEAMLAAYG
                     SGAARASHDEGKRILNVDIGGGTTKLAVVEGGRIVGMAAVHIGGRLQVVDAEDRIVRL
                     DPAGRHHAAVAGFAWKVGDCAPRADMARVADGMADALIAAIRERPLPQAIEHLYLTDP
                     IAEPGRIDGVMFSGGVGEYVYGRESRDFGDMGKLLGTAIARRLSDGALPWPLLPPHEC
                     IRATAIGASEYSVQLSGNTIYISSPGDLLPRKNLQVLLPPYACGEEIVAEELARTIRS
                     HFTAFDLEEGASEVVLAFRWSGAPSYPRIAAFARGIVQGLPRTIVSGKPIFLVLDGDI
                     AQTLGAILREELKLANEILVIDGVTLWDLDYIDLGRIRLPSHTVPVTIKSLVFSEDPR
                     HPNDPGHHHGHAHHHHDHDHDHGHHHDHNHHHHDGDHHPHHHVKPGKKTWKR"
     gene            1324..>2030
                     /locus_tag="AC16CX_21120"
     CDS             1324..>2030
                     /locus_tag="AC16CX_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3015044.1"
                     /translation="MEAVAGRHGRPGTTAAPFRIDLKWVVLGACVVAVAYLAVIPLGF
                     LLWQSFFTPQTAAKPAVFTLQNYATAYTSLETAKLFLNSLLFAIGTATFSLVVGTALA
                     WMNERTNTPFKRLFFGLALVPLIIPGILFTISWIMLASPKIGIFNLLLQGWFGTDAVF
                     FNVYTLSGMIWVDGLHYSPMAFLLMTAAFRSMDPALEESASMSGANLAQVVRRITLAL
                     SWPAIFATLLILFVRAI"
BASE COUNT          332 a          690 c          654 g          354 t
ORIGIN      
        1 ggtgattctc accggcgagg cgttgcgccg gcagaacgcg gaggcgatcg cggggctcct
       61 cgccgaaaac ggcggggagt tcgtgtgcgc ggccgccggc catcacctcg aggcgatgct
      121 cgccgcctac ggctcgggcg cggcgcgcgc atcacacgac gagggcaagc gcatcctcaa
      181 cgtggacatc ggcggcggca cgaccaagct ggcggtggtg gaaggcggca ggatcgtcgg
      241 catggcggcg gtgcacatcg gcgggcggct gcaggtggtg gatgccgagg accgcatcgt
      301 gcgcctcgat cccgctggac gccatcacgc cgcggtggca ggcttcgcgt ggaaggtcgg
      361 cgactgtgcg ccgcgcgcgg acatggcgcg cgtcgcggac gggatggcgg atgcgctgat
      421 cgcggcgatc cgcgagcggc ccctgccgca agcgatcgag cacctctacc tcaccgaccc
      481 gatcgcggag cccggccgca tcgacggcgt gatgttctcc ggcggcgtcg gcgaatacgt
      541 gtacggccgg gaatcccgcg attttggcga catgggcaag ctcctcggca ctgcgatcgc
      601 gcgccgcctc tcggacgggg cgctgccgtg gccgctgctg ccaccgcacg aatgcatacg
      661 cgcgaccgcc atcggcgcct cggaatacag cgtgcagctc tccggcaaca cgatctacat
      721 ctcgagcccg ggtgatctgc tgccccgcaa gaacctgcag gtgctgctgc cgccgtatgc
      781 gtgcggggag gagatcgtcg cggaagagct cgcacgcacc atacgcagcc acttcaccgc
      841 gttcgacctc gaggagggcg catccgaggt cgtgctcgcg ttccgctgga gcggcgcgcc
      901 ttcctacccg cgcatcgccg ccttcgcgcg tggcatcgtc cagggtctgc cgcggaccat
      961 cgtgagcggc aagccgatct tcctggtact cgacggcgac atcgcgcaga ccctgggcgc
     1021 gatactgcgc gaggagctga agctcgcaaa cgagatcctg gtgatcgacg gcgtgacctt
     1081 gtgggacctc gactacattg accttggccg catccggctc ccctcgcaca cggtgccggt
     1141 cacgatcaag tcgctggtgt tcagcgagga tccccgccat ccgaacgacc cgggccacca
     1201 tcatggtcat gcgcatcatc accatgatca tgaccacgat cacggtcatc atcatgacca
     1261 taatcatcac catcatgacg gcgatcatca ccctcatcac cacgtaaagc ccggaaagaa
     1321 aacttggaag cggtagctgg ccggcacggc agaccgggga cgacagccgc ccccttcaga
     1381 atagatctga aatgggtggt gctcggcgcg tgcgtagtcg ccgtcgccta tctcgcggtg
     1441 atccccctgg gattcctgct gtggcagagc ttcttcacgc cgcagacggc ggcgaagccg
     1501 gccgtgttca cgctgcagaa ttacgccacg gcgtacacca gcctggagac ggcgaagctt
     1561 ttcctcaact cgctgctgtt cgccatcgga acggccacgt tctcgcttgt cgtcggcacg
     1621 gcgctcgcgt ggatgaacga gcgcaccaac acgccgttca agcggctgtt cttcgggctc
     1681 gccctcgtgc ccctgatcat ccccgggatc cttttcacca tctcctggat catgctcgcc
     1741 agccccaaga tcgggatctt caatctcctg ctgcaaggct ggttcggcac cgacgccgtg
     1801 ttcttcaacg tctacaccct gtcgggcatg atttgggtgg acgggctgca ctactcgccg
     1861 atggcgtttt tgctcatgac cgcggcgttc cgctcaatgg accccgcgct cgaggaatcg
     1921 gcttcgatga gcggggcgaa cctcgcccag gtggtgcggc gcatcacgct ggcgctttcc
     1981 tggcccgcga tcttcgcgac gctgctgatt ctcttcgtgc gcgcgatcga
//
